title: UV irradiation-induced interleukin-1 and basic fibroblast growth factor synthesis and release mediate part of the UV response.
abstract: UV irradiated cells release into the culture medium factors that induce, when given to nonirradiated cells, the transcription of several UV-inducible genes (collagenase I, human immunodeficiency virus type 1, metallothionein IIA). We identify here the active factors released from UV-treated HeLa cells, as interleukin 1 alpha and basic fibroblast growth factor. UV irradiation leads to increased mRNA levels for both factors and to their enhanced synthesis. Experiments with the drug suramin, which inhibits growth factor-growth factor receptor interactions and with antibodies directed against interleukin 1 alpha and basic fibroblast growth factor, suggest that growth factors do not only transduce the UV-induced signal to nonirradiated cells but act on the producer cell thus establishing an obligatory growth factor loop for at least part of the UV response.
authors.affiliation: Kernforschungszentrum Karlsruhe, Institut für Genetik und Toxikologie, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Krämer
authors.forename: C
authors.initials: C
authors.lastname: Sachsenmaier
authors.forename: P
authors.initials: P
authors.lastname: Herrlich
authors.forename: H J
authors.initials: HJ
authors.lastname: Rahmsdorf
mesh_terms: D000906:Antibodies / Q000276:immunology; D015152:Blotting, Northern; D017364:Collagenases / Q000235:genetics / Q000378:metabolism; D003470:Culture Media; D016222:Fibroblast Growth Factor 2 / Q000096:biosynthesis* / Q000276:immunology; D005786:Gene Expression Regulation / Q000528:radiation effects; D006367:HeLa Cells; D006801:Humans; D007375:Interleukin-1 / Q000096:biosynthesis* / Q000276:immunology; D013498:Suramin / Q000494:pharmacology; D014158:Transcription, Genetic / Q000187:drug effects / Q000528:radiation effects; D014466:Ultraviolet Rays
chemical_list: D000906:Antibodies; D003470:Culture Media; D007375:Interleukin-1; D016222:Fibroblast Growth Factor 2; D013498:Suramin; D017364:Collagenases