title: Downregulation of Ke 6, a novel gene encoded within the major histocompatibility complex, in murine polycystic kidney disease.
abstract: Polycystic kidney disease (PKD) is characterized by progressive enlargement of the kidneys due to numerous expanding cysts ultimately leading to renal failure. We have identified a gene, Ke 6, located within the H-2K/tw5 region on mouse chromosome 17, which is downregulated in two distinct murine models of heritable PKD. Ke 6 is a member of the short-chain alcohol dehydrogenase family and possess remarkable amino acid sequence conservation with several bacterial proteins with oxidoreductase function. The Ke 6 gene gives rise to two transcripts--a 1-kb Ke 6a mRNA which is abundant in kidney and liver tissue and a 1.4-kb Ke 6b mRNA which is found at a moderate level in spleen tissue. We report here the complete nucleotide sequence of Ke 6a cDNA and the expression of the Ke 6 gene in murine models of PKD. The Ke 6 gene may be intimately involved in the manifestation of these cystic kidney diseases.
authors.affiliation: Department of Medicine, Brigham and Women's Hospital, Boston, Massachusetts.
authors.forename: N
authors.initials: N
authors.lastname: Aziz
authors.forename: M M
authors.initials: MM
authors.lastname: Maxwell
authors.forename: B
authors.initials: B
authors.lastname: St Jacques
authors.forename: B M
authors.initials: BM
authors.lastname: Brenner
mesh_terms: D000426:Alcohol Dehydrogenase / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D002474:Cell-Free System; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D003360:Cosmids / Q000235:genetics; D004195:Disease Models, Animal; D015536:Down-Regulation; D006649:Histocompatibility Antigens; D008285:Major Histocompatibility Complex / Q000235:genetics*; D051379:Mice; D008817:Mice, Mutant Strains / Q000235:genetics*; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000235:genetics; D007690:Polycystic Kidney Diseases / Q000209:etiology / Q000235:genetics*; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D011922:Rats, Mutant Strains / Q000235:genetics; D016415:Sequence Alignment; D014018:Tissue Distribution
chemical_list: D001426:Bacterial Proteins; C079876:H2-Ke6 protein, mouse; D006649:Histocompatibility Antigens; D012333:RNA, Messenger; D010088:Oxidoreductases; D000426:Alcohol Dehydrogenase