title: Actin-binding protein is a component of bovine erythrocytes.
abstract: Actin-binding protein (ABP) is a well-characterized polypeptide capable of crosslinking filamentous actin. To date, this polypeptide has been shown to exist in a number of tissues and cultured cell lines. This report shows that by using a panel of three monoclonal antibodies for immunoblotting and immunofluorescence analysis, that ABP is present in bovine erythrocytes. Moreover, the data obtained suggest that this protein is a component of the erythrocyte membrane skeleton. Additionally, bovine erythrocyte ABP is shown to possess both an apparent molecular weight and an isoelectric point identical to that of bovine smooth muscle filamin, implying that these two polypeptides are identical.
authors.affiliation: Department of Cell Biology, School of Medicine and Dentistry, University of Alabama, Birmingham.
authors.forename: K D
authors.initials: KD
authors.lastname: Brown
authors.forename: R P
authors.initials: RP
authors.lastname: Zinkowski
authors.forename: S E
authors.initials: SE
authors.lastname: Hays
authors.forename: L I
authors.initials: LI
authors.lastname: Binder
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001011:Aorta; D002417:Cattle / Q000097:blood*; D003285:Contractile Proteins / Q000097:blood*; D004910:Erythrocyte Membrane / Q000737:chemistry*; D064448:Filamins; D015151:Immunoblotting; D007526:Isoelectric Point; D008838:Microcomputers; D008840:Microfilament Proteins / Q000097:blood*; D008856:Microscopy, Fluorescence; D008970:Molecular Weight; D009124:Muscle Proteins / Q000032:analysis; D009131:Muscle, Smooth, Vascular / Q000737:chemistry; D014741:Video Recording
chemical_list: D000911:Antibodies, Monoclonal; D003285:Contractile Proteins; D064448:Filamins; D008840:Microfilament Proteins; D009124:Muscle Proteins