Crystallization and preliminary crystallographic data for formyltetrahydrofolate synthetase from Clostridium thermoaceticum.
Formyltetrahydrofolate synthetase from Clostridium thermoaceticum has been crystallized using the hanging-drop method from ammonium sulphate and PEG solutions. Crystals are trigonal, the space group is R32, a = 163 A, c = 259 A. A 4.2 A resolution data set has been collected. Analysis of the data using the self-rotation function shows that tetramers have approximate 222 symmetry and are positioned on a crystallographic 2-fold axis.
Lewinski
K
K
Department of Chemistry and Biochemistry, University of South Carolina, Columbia 29208.
Hui
Y
Y
Jakob
C G
CG
Lovell
C R
CR
Lebioda
L
L
D003013:Clostridium / Q000201:enzymology*; D003460:Crystallization; D005574:Formate-Tetrahydrofolate Ligase / Q000737:chemistry*; D014961:X-Ray Diffraction
D005574:Formate-Tetrahydrofolate Ligase