Size-exclusion chromatographic determination of beta-glucan with postcolumn reaction detection.
Using the specific interaction of beta-glucan with calcofluor dye as a detection method for size-exclusion chromatographic analysis, very simple direct extractions can be used for sample preparation. Either fluorescence or UV detection is employed. The calibration graphs are linear between 200 and 20 mg/l, although much more sensitive analyses are possible. The response remained independent of relative molecular mass in the range studied (1.7 x 10(6)-1.85 x 10(5)). Results for barley and malt show good correlation with those obtained by flow-injection analysis using calcofluor.
Suortti
T
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Technical Research Centre of Finland, Food Research Laboratory, Espoo.
D002850:Chromatography, Gel / Q000379:methods*; D002523:Edible Grain / Q000737:chemistry; D005936:Glucans / Q000032:analysis*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
D005936:Glucans