Determination of 5,10,15,20-tetra-(m-hydroxyphenyl)chlorin in human plasma by high performance liquid chromatography.
A high performance liquid chromatographic method for the determination of the photodynamic chemotherapeutic agent 5,10,15,20-tetra(m-hydroxyphenyl)chlorin (m-THPC) in human plasma following intravenous infusion is described. The procedure involves extraction of the drug in plasma with methanol/dimethyl sulphoxide (4:1 v/v) containing 5,10,15,20-tetra(p-hydroxyphenyl)chlorin as the internal standard and separation on a C18 reversed phase column with acetonitrile:0.1% trifluoroacetic acid (77:23 v/v) as the mobile phase. The drug was detected specifically and sensitively at its absorption maximum of 423 nm with a detection limit of 15 ng/mL (signal-to-noise ratio of 5). The intra- and inter-assay coefficients of variation (CV) on analysis of a plasma spiked with m-THPC (1 micrograms/mL) were 2.3 and 3.4% (n = 6), respectively.
Wang
Q
Q
MRC Toxicology Unit, Medical Research Council Laboratory, Carshalton, Surrey, UK.
Ris
H B
HB
Altermatt
H J
HJ
Reynolds
B
B
Stewart
J C
JC
Bonnett
R
R
Lim
C K
CK
D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007202:Indicators and Reagents; D007262:Infusions, Intravenous; D008652:Mesoporphyrins / Q000008:administration & dosage / Q000097:blood*; D008654:Mesothelioma / Q000097:blood; D012015:Reference Standards; D012997:Solvents
D000970:Antineoplastic Agents; D007202:Indicators and Reagents; D008652:Mesoporphyrins; D012997:Solvents; C072269:temoporfin