title: Parvovirus B19-specific DNA in bone marrow from B19 arthropathy patients: evidence for B19 virus persistence.
abstract: To determine if B19 infection persists in patients with chronic B19 arthropathy, acute B19 infection in adults was documented by IgM serology. Bone marrow aspirates were obtained 24-42 months after acute infection in 4 patients who developed chronic joint symptoms. DNA from bone marrow aspirates was amplified by polymerase chain reaction by using B19-specific DNA sequences in the viral capsid gene and probing with B19-specific oligonucleotides in Southern analysis. B19-specific DNA sequences were detected in all 4 chronic arthropathy patients compared with 0 of 7 anti-B19 IgM-, IgG- and 0 of 6 anti-B19 IgM-, IgG+ normal bone marrow donors. Three patients with serologically proven acute B19 infection and acute but nonchronic joint symptoms had B19 DNA detected in bone marrow aspirates 2-18 months after infection. These findings suggest that B19 arthropathy is associated with persistence of either B19 virus or select B19 DNA sequences.
authors.affiliation: Department of Internal Medicine, University of Iowa College of Medicine, Iowa City.
authors.forename: F
authors.initials: F
authors.lastname: Foto
authors.forename: K G
authors.initials: KG
authors.lastname: Saag
authors.forename: L L
authors.initials: LL
authors.lastname: Scharosch
authors.forename: E J
authors.initials: EJ
authors.lastname: Howard
authors.forename: S J
authors.initials: SJ
authors.lastname: Naides
mesh_terms: D001170:Arthritis, Infectious / Q000209:etiology / Q000382:microbiology*; D001483:Base Sequence; D015139:Blotting, Southern; D001853:Bone Marrow / Q000382:microbiology*; D002908:Chronic Disease; D004279:DNA, Viral / Q000032:analysis*; D016731:Erythema Infectiosum / Q000150:complications / Q000175:diagnosis*; D006801:Humans; D008969:Molecular Sequence Data; D016732:Parvovirus B19, Human / Q000235:genetics* / Q000502:physiology; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity
chemical_list: D004279:DNA, Viral