title: Precise area determination of skin-prick tests: validation of a scanning device and software for a personal computer.
abstract: When skin-prick tests (SPTs) are used quantitatively, the circumference of the weal and/or the flare is outlined using a felt tip pen, and transferred to paper by adhesive tape. The aim of the study was to develop and validate a procedure, objectively and precisely determining these areas after transfer to paper. A system was developed enabling the drawing of the area of weal or flare to be read by a hand-held scanner and calculated on a personal computer. Areas in the 5-500 mm2 range could be determined with day-to-day and interoperator coefficients of variation (CVs) of 3.1% and 1.8%, respectively. Accuracy was determined in two ways: by correlation to cutting/weighing of four times enlarged SPT areas (r2 = 0.999, P < 0.001) and by measuring standardized areas (deviations less than intra-assay CV, i.e. 1-2%). For comparison, CV of alternative methods were also determined: eight different areas (9-76 mm2) were evaluated in quadruplicate using the SPT-scanner (CV = 1.4%), by cutting/weighing of paper (CV = 2.3%), by digitizing (CV = 4.4%) or by measuring longest and orthogonal diameters (CV = 13.6%). In conclusion, the scanning device and software provides an objective and reproducible procedure for rapid determination of SPT areas. When areas are determined by scanning, digitizing or cutting/weighing the variations in area determination becomes negligible compared to the variations of the entire skin test procedure.
authors.affiliation: Laboratory of Medical Allergology, National University Hospital, Copenhagen, Denmark.
authors.forename: L K
authors.initials: LK
authors.lastname: Poulsen
authors.forename: C
authors.initials: C
authors.lastname: Liisberg
authors.forename: C
authors.initials: C
authors.lastname: Bindslev-Jensen
authors.forename: H J
authors.initials: HJ
authors.lastname: Malling
mesh_terms: D003936:Diagnosis, Computer-Assisted / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D008838:Microcomputers; D015203:Reproducibility of Results; D012882:Skin Tests / Q000379:methods*; D012984:Software