Growth factor for cardiac hypertrophy.
Cardiac size can be regulated by the balance in activity between cardiac growth factors and inhibiting factors, chalones. This study was undertaken to verify the role of the cardiac growth factor and its purification from hypertrophied hearts. For this propose the hypertrophied hearts of renovascular hypertensive rats were used. The purification was made by using an isoelectric focusing chromatography and the HPLC method. We examined the cardiac growth effect of the isolated fractions with cultured chicken embryonic cardiac myocytes. Simultaneously, the influence of these fractions on the cardiac cell cycle was examined by DNA analysis with the flow cytometric method. If the hearts were overloaded due to hypertension, the growth of the cardiac size could be induced by increased the level of five proteins with different molecular weight and with an isoelectric point of 8.3. The significant growth activities were observed at these five proteins compared to the absence of the fractions. For the appearance of these growth effect, it is necessary that the structure of the protein is there fundamentally as a form with a molecular weight of 27 k dalton. After addition of these isolated fractions, BrdU content is S and G2 phases by flow cytometry was increased. This change indicates that the cardiac myocytes are stimulated in form DNA synthesis.
Nagano
M
M
Department of Internal Medicine, Jikei University School of Medicine, Tokyo, Japan.
Ohkubo
T
T
Arino
T
T
Tsuchiya
M
M
Nagai
M
M
D000818:Animals; D001973:Bromodeoxyuridine / Q000378:metabolism; D002312:Cardiomyopathy, Hypertrophic / Q000378:metabolism*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured / Q000187:drug effects; D002642:Chick Embryo; D004195:Disease Models, Animal; D016195:G2 Phase; D006133:Growth Substances / Q000302:isolation & purification* / Q000494:pharmacology; D006321:Heart / Q000187:drug effects / Q000196:embryology; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D016196:S Phase; D014020:Tissue Extracts / Q000494:pharmacology
D006133:Growth Substances; D014020:Tissue Extracts; D001973:Bromodeoxyuridine