Neutralizing antibodies against the peptide antibiotic AS-48: immunocytological studies.
Antisera against the broad-spectrum peptide antibiotic AS-48 produced by Enterococcus faecalis were obtained from immunized rabbits. Appreciable antibody titers were obtained only after repeated immunization, suggesting a feeble antigenicity for AS-48. Upon incubation with AS-48, the antisera neutralized its bacteriolytic action on E. faecalis S-47, although the simultaneous addition of AS-48 and serum did not prevent lysis. Crude serum cross-reacted with outer envelope components of enterococci, although specific anti-AS-48 antibodies, purified by affinity chromatography, reacted only with AS-48-treated cells. Labelling with immunofluorescence and colloidal gold particles was carried out on sensitive and resistant bacterial species to determine the interaction of AS-48 with cell structures.
Maqueda
M
M
Departamento de Microbiología, Facultad de Ciencias, Universidad de Granada, Spain.
Gálvez
A
A
Martínez-Bueno
M
M
Guerra
I
I
Valdivia
E
E
D000818:Animals; D000900:Anti-Bacterial Agents / Q000276:immunology* / Q000494:pharmacology; D001412:Bacillus subtilis / Q000187:drug effects; D001426:Bacterial Proteins; D003101:Collodion; D013293:Enterococcus faecalis / Q000187:drug effects / Q000276:immunology*; D004926:Escherichia coli / Q000187:drug effects; D007150:Immunohistochemistry; D008567:Membranes, Artificial; D008826:Microbial Sensitivity Tests; D009500:Neutralization Tests; D010455:Peptides / Q000276:immunology / Q000494:pharmacology; D011817:Rabbits
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D008567:Membranes, Artificial; D010455:Peptides; C051034:BacA protein, Enterococcus faecalis; D003101:Collodion