title: The use of thermostable Vero cell-adapted rinderpest vaccine as a heterologous vaccine against peste des petits ruminants.
abstract: The thermostable Vero cell-adapted rinderpest vaccine was evaluated in terms of immunogenicity as a heterologous vaccine against peste des petits ruminants. A titration to establish the minimum immunising dose was performed in American mixed breed goats by vaccinating test subjects with dilutions of Vero cell-adapted rinderpest vaccine and then challenging 26 days later with virulent peste des petits ruminants virus. All animals were followed for virus neutralising antibodies against both rinderpest and peste des petits ruminants virus after vaccination and challenge. The antibody response to vaccination was primarily against rinderpest virus with very low levels of cross-reactivity to peste des petits ruminants virus. Following challenge, animals which possessed anti-rinderpest neutralising antibodies remained clinically normal but mounted strong anti-peste des petits ruminants virus neutralising antibody responses indicating that replication of challenge virus took place without the induction of illness. The 50 per cent minimum goat immunising dose was 3 tissue culture infectious doses 50 per cent (TCID50) as established by serological response and protection against challenge. The thermostable Vero cell-adapted rinderpest vaccine is a suitable immunogen for the protection of goats against peste des petits ruminants.
authors.affiliation: Tufts University School of Veterinary Medicine, Section of International Veterinary Medicine, North Grafton, Massachusetts 01536.
authors.forename: J C
authors.initials: JC
authors.lastname: Mariner
authors.forename: J A
authors.initials: JA
authors.lastname: House
authors.forename: C A
authors.initials: CA
authors.lastname: Mebus
authors.forename: M C
authors.initials: MC
authors.lastname: van den Ende
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D002478:Cells, Cultured; D003429:Cross Reactions / Q000276:immunology; D015511:Goat Diseases / Q000382:microbiology / Q000517:prevention & control*; D006041:Goats; D006358:Hot Temperature; D012301:Rinderpest / Q000276:immunology / Q000517:prevention & control*; D012302:Rinderpest virus / Q000276:immunology*; D014611:Vaccination / Q000662:veterinary*; D014709:Vero Cells; D014765:Viral Vaccines / Q000008:administration & dosage* / Q000276:immunology; D014779:Virus Replication
chemical_list: D000914:Antibodies, Viral; D014765:Viral Vaccines