Blood donations indeterminate in HIV-1 western blot analysed by IgM immunoblot and polymerase chain reaction.
The presence of IgM antibodies to human immunodeficiency virus 1 (HIV-1) was investigated in blood donor sera which were indeterminate in anti-HIV-1 IgG Western blot testing. In 7 of 173 instances out of approximately 1,000,000 blood donation sera with an isolated anti-p24 IgG produced an anti-gp41-45 IgM immunoblot reaction. Applying polymerase chain reaction (PCR) to 29 indeterminate samples out of approximately 125,000 blood donations it was found that 2 of them were IgM-positive and also contained HIV-1-specific DNA sequences. Eleven months later 1 of these 2 donors was retested and found IgM and PCR negative.
Knüver-Hopf
J
J
German Red Cross Blood Transfusion, Springe.
Heinze
H
H
Lambrecht
B
B
Mohr
H
H
Beyer
J
J
Schmitt
H
H
D001782:Blood Donors; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D015683:Gene Products, gag / Q000276:immunology; D005858:Germany / Q000453:epidemiology; D015483:HIV Antibodies / Q000097:blood*; D016655:HIV Core Protein p24 / Q000276:immunology; D015528:HIV Seroprevalence; D015497:HIV-1 / Q000302:isolation & purification*; D015498:HIV-2 / Q000276:immunology; D006801:Humans; D015151:Immunoblotting; D007074:Immunoglobulin G / Q000276:immunology; D007075:Immunoglobulin M / Q000097:blood*; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D054301:gag Gene Products, Human Immunodeficiency Virus
D015683:Gene Products, gag; D015483:HIV Antibodies; D016655:HIV Core Protein p24; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D054301:gag Gene Products, Human Immunodeficiency Virus; C061169:p18 gag protein, Human immunodeficiency virus 1