Interferon-gamma promotes proliferation of rat skeletal muscle cells in vitro and alters their AChR distribution.
Recombinant interferon-gamma (IFN-gamma) caused a dose-dependent increase in the proliferation of myoblasts in cultures of rat skeletal muscles as determined by bromodeoxyuridine incorporation. As a result of this proliferation the number of myotubes increased in the cultures, while the fusion index was unchanged. In myotubes exposed to IFN-gamma there was a change in the distribution of nicotinic acetylcholine receptors (nAChR) as detected by binding of FITC-conjugated alpha-bungarotoxin with a significant decrease in the number of linear aggregates and an increase in diffusely distributed receptors.
Kelić
S
S
Clinical Research Center, Karolinska Institutet, Huddinge Hospital, Sweden.
Olsson
T
T
Kristensson
K
K
D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002459:Cell Fusion / Q000187:drug effects; D002467:Cell Nucleus / Q000187:drug effects; D005260:Female; D066298:In Vitro Techniques; D007371:Interferon-gamma / Q000494:pharmacology*; D008870:Microtubules / Q000187:drug effects; D009132:Muscles / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley; D011978:Receptors, Nicotinic / Q000187:drug effects
D011978:Receptors, Nicotinic; D007371:Interferon-gamma; D000110:Acetylcholinesterase