DIF-resistant expression of a prespore-specific gene in Dictyostelium in vitro development.
In submerged culture, the prespore-specific gene, D19, of Dictyostelium discoideum was found to be expressed in the early stages of development, even in the presence of 1 nM of DIF-1 (stalk differentiation inducing factor). This concentration of DIF-1 later caused the degradation of the previously accumulated D19 mRNA concomitant with the induction of the prestalk-specific genes ecmA/ecmB and eventually 85% of cells differentiated into stalk cells. These results suggest that prespore differentiation occurs at least transiently even in the presence of DIF-1.
Kubohara
Y
Y
Department of Botany, Faculty of Science, Kyoto University, Japan.
Okamoto
K
K
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics; D004023:Dictyostelium / Q000235:genetics*; D006588:Hexanones / Q000494:pharmacology*; D015398:Signal Transduction
D006588:Hexanones; C053433:1-((3,5-dichloro)-2,6-dihydroxy-4-methoxyphenyl)-1-hexanone