Rapid identification of Renibacterium salmoninarum using an oligonucleotide probe complementary to 16S rRNA.
Bacterial kidney disease in salmonid fish is caused by the slow-growing Gram-positive rod, Renibacterium salmoninarum. The partial sequence of 16S rRNA from R. salmoninarum was determined and compared with published bacterial 16S rRNA sequences. From this sequence information, a 30-bases-long oligonucleotide was designed and used as a specific probe for identification of R. salmoninarum in filter hybridization experiments. Strong specific hybridization signals were observed for all strains of R. salmoninarum tested. Furthermore, no cross-hybridization could be seen against 22 other bacterial species, among them other salmonid fish pathogens. The detection limit for the probe in direct filter hybridization by the dot-blot technique was 2.5 x 10(4) bacteria. It was also possible to detect R. salmoninarum in clinical samples by direct filter hybridization.
Mattsson
J G
JG
National Veterinary Institute, Uppsala, Sweden.
Gersdorf
H
H
Jansson
E
E
Hongslo
T
T
Göbel
U B
UB
Johansson
K E
KE
D000818:Animals; D001419:Bacteria / Q000235:genetics; D001483:Base Sequence; D015342:DNA Probes; D004269:DNA, Bacterial / Q000032:analysis*; D004275:DNA, Ribosomal / Q000032:analysis*; D005393:Fish Diseases / Q000175:diagnosis / Q000382:microbiology*; D016908:Gram-Positive Bacterial Infections / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D016987:Gram-Positive Rods / Q000235:genetics / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D009393:Nephritis / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D009693:Nucleic Acid Hybridization; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D012487:Salmonidae / Q000382:microbiology*; D012680:Sensitivity and Specificity; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
D015342:DNA Probes; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S