title: New algorithm for analysis of data obtained by means of circular dichroism titration method.
abstract: A new method for analysis of circular dichroism titration data used for drug-protein binding investigations is proposed. A square equation between molar ellipticity change and total drug concentration is obtained which is examined analytically. A new minimization algorithm for determining the binding sites and association constants of each type of drug-protein complexes is applied.
authors.affiliation: Department of Physics and Biophysics, Medical Academy, Sofia, Bulgaria.
authors.forename: N
authors.initials: N
authors.lastname: Stavreva
authors.forename: V
authors.initials: V
authors.lastname: Ruseva
authors.forename: D
authors.initials: D
authors.lastname: Michailova
authors.forename: P
authors.initials: P
authors.lastname: Stavrev
mesh_terms: D000465:Algorithms; D002942:Circular Dichroism; D004364:Pharmaceutical Preparations / Q000737:chemistry / Q000378:metabolism; D011485:Protein Binding; D011955:Receptors, Drug / Q000378:metabolism
chemical_list: D004364:Pharmaceutical Preparations; D011955:Receptors, Drug