title: Differential expression of the insulin-like growth factor II and transthyretin genes in the developing rat choroid plexus.
abstract: Choroid plexus (CP) development may depend on an inductive interaction between primordial CP epithelium and the overlying mesenchyme. Expression of the two CP epithelial-expressed genes, transthyretin (TTR) and insulin-like growth factor II (IGF-II), were studied by in situ hybridization in the developing rat. Transthyretin mRNA is expressed in abundance in the primordial CP epithelium prior to CP morphogenesis (e10-11) but IGF-II mRNA expression begins later (e13) and increases gradually as morphogenesis proceeds. In the CP stroma (mesenchyme), IGF-II mRNA is abundant prior to CP morphogenesis but decreases as embryogenesis proceeds and is absent in the adult. Our findings suggest that IGF-II may play an early paracrine and later autocrine role in CP development. A model is proposed in which IGF-II synthesized by mesenchyme serves as an inducing principle for CP epithelial differentiation.
authors.affiliation: Department of Neurology, Columbia University, College of Physicians and Surgeons, New York, NY.
authors.forename: T
authors.initials: T
authors.lastname: Cavallaro
authors.forename: R L
authors.initials: RL
authors.lastname: Martone
authors.forename: F
authors.initials: F
authors.lastname: Stylianopoulou
authors.forename: J
authors.initials: J
authors.lastname: Herbert
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002831:Choroid Plexus / Q000166:cytology / Q000196:embryology* / Q000502:physiology; D005314:Embryonic and Fetal Development; D005260:Female; D015870:Gene Expression; D005865:Gestational Age; D017403:In Situ Hybridization; D007335:Insulin-Like Growth Factor II / Q000235:genetics*; D009024:Morphogenesis; D011228:Prealbumin / Q000235:genetics*; D011247:Pregnancy; D015347:RNA Probes; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D011228:Prealbumin; D015347:RNA Probes; D012333:RNA, Messenger; D007335:Insulin-Like Growth Factor II