Control of G1 to S cell cycle progression of Trypanosoma brucei S427cl1 organisms under axenic conditions.
Trypanosoma brucei S427cl1 organisms made 6 divisions in modified minimal essential medium (BMEM) supplemented with fetal bovine serum (FBS)-low or high density lipoprotein (LDL, HDL) and fatty acid-free bovine serum albumin (FAF-BSA). Omission of lipoproteins or FAF-BSA from the medium caused the parasites to accumulate in G1 of the cell cycle and to lose the ability to replicate at 37 degrees C. Proteinase K-treated LDL or HDL, which did not have detectable apolipoprotein, supported the G1 to S cell cycle transition of T. brucei S427cl1 organisms in BMEM supplemented with FAF-BSA. Addition of C6:0, C7:0 or fatty C8:0 fatty acid (1 mol fatty acid mol-1 FAF-BSA in the incubation mixture) to serum-free medium supplemented with LDL or HDL and FAF-BSA prevented T. brucei S427cl1 organisms from progressing through G1 into S of the cell cycle. T. brucei S427cl1 organisms became stumpy-like forms during plateau phase growth under axenic conditions. Stumpy-like T. brucei S427cl1 organisms were mainly in G1 of the cell cycle, expressed raised levels of NAD diaphorase activity, were unable to replicate at 37 degrees C, but were able to differentiate to replicating procyclic organisms. Medium collected from plateau phase cultures of T. brucei S427cl1 did not support the G1 to S cell cycle transition of exponentially growing T. brucei organisms. The capacity of plateau phase medium to support G1 to S transition of T. brucei S427cl1 organisms was restored by addition of FAF-BSA and its capacity to support 4 cycles of replication of the parasites was restored by addition of FAF-BSA and LDL or HDL.
Morgan
G A
GA
Department of Microbiology, Ohio State University, Columbus 43210-1292.
Laufman
H B
HB
Otieno-Omondi
F P
FP
Black
S J
SJ
D000818:Animals; D002453:Cell Cycle; D002455:Cell Division; D003470:Culture Media; D016054:DNA, Protozoan / Q000032:analysis; D007399:Interphase; D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D016196:S Phase; D012710:Serum Albumin, Bovine; D014346:Trypanosoma brucei brucei / Q000737:chemistry / Q000166:cytology* / Q000254:growth & development
D003470:Culture Media; D016054:DNA, Protozoan; D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D012710:Serum Albumin, Bovine