Isolation and characterization of a yeast gene that is homologous with a meiosis-specific cDNA from a plant.
By using as probe a meiosis-specific cDNA clone LIM15 from the monocotyledonous plant, Lilium longiflorum, a clone containing a 2.8 kb DNA fragment was isolated from a genomic library of Saccharomyces cerevisiae. Primary structure analysis revealed that the clone includes two complete open reading frames, designated ISC2 and ISC10, capable of coding for a 36.6 kDa and a 31.6 kDa polypeptide, respectively, with the former frame being interrupted by a 92 bp intron. The predicted amino acid sequence of Isc2 was 56% identical with the putative gene product of lily cDNA clone LIM15, and showed limited sequence similarity with the yeast RAD57 gene product. Transcripts of the two genes begin accumulating 2.5 h and 7.5 h after induction of meiosis, respectively, according to a Northern hybridization analysis. Since disruption of either one of these genes had a drastic effect on the ability to form spores, ISC2 and ISC10 are expected to play significant roles in the formation of reproductive cells.
Kobayashi
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T
Department of Biology, School of Science, Nagoya University, Japan.
Hotta
Y
Y
Tabata
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D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D002453:Cell Cycle / Q000235:genetics; D004252:DNA Mutational Analysis; D015342:DNA Probes / Q000235:genetics; D005800:Genes, Fungal / Q000235:genetics*; D017343:Genes, Plant / Q000235:genetics*; D016681:Genome, Fungal; D007438:Introns / Q000235:genetics; D008540:Meiosis / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics*; D017421:Sequence Analysis; D017386:Sequence Homology, Amino Acid; D013172:Spores, Fungal / Q000235:genetics; D014158:Transcription, Genetic
D015342:DNA Probes; D012333:RNA, Messenger