Morphology and somatotopy of the central arborizations of rapidly adapting glabrous skin afferents in the rat lumbar spinal cord.
The central arborizations in the dorsal horn of the spinal cord of 23 rapidly adapting (RA) A-beta primary afferent neurons innervating different regions of the glabrous skin of the hindpaw were studied by the intra-axonal injection of horseradish peroxidase in adult rats. A total of 284 arbors of the complex, simple, and blind-ending variety were recovered. The arbors of RA afferents innervating the toes, paw pads, and non-pad hindpaw differed from each other in branch pattern and dimensions. The simple and complex arbors, which are both bouton-containing, were distributed mainly in laminae III-V, although some complex arbors projected dorsally into lamina IIi. The hindpaw glabrous skin afferent terminals were located in the lumbar enlargement from caudal L3 to rostral L6. A crude somatotopic organization was observed such that toes 1-5 were represented successively in more caudal positions from mid-L4 to caudal L5. The paw pads were organized in a rostrocaudal sequence moving from the paw pads proximal to toe 1 across the foot to the paw pads proximal to toe 5, from caudal L3 to mid-L5. Non-pad hindpaw afferents were located in caudal L5. Overlap between toe, paw pad and non-pad afferent central fields was present, however, and the central terminals of afferents with non-adjacent peripheral receptive fields were shown to occupy the same region of the dorsal horn.
Shortland
P
P
Department of Anatomy and Developmental Biology, University College London, England.
Woolf
C J
CJ
D000222:Adaptation, Physiological; D000818:Animals; D001931:Brain Mapping / Q000379:methods*; D005260:Female; D005528:Foot / Q000294:innervation; D006614:Hindlimb; D008161:Lumbosacral Region; D008297:Male; D009411:Nerve Endings / Q000648:ultrastructure*; D009475:Neurons, Afferent / Q000648:ultrastructure*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000294:innervation*; D013116:Spinal Cord / Q000502:physiology* / Q000648:ultrastructure; D013997:Time Factors; D014034:Toes / Q000294:innervation