title: Determination of clozapine and desmethylclozapine in human plasma by high-performance liquid chromatography with ultraviolet detection.
abstract: A method using reversed-phase high-performance liquid chromatography for the simultaneous determination of clozapine and its desmethyl metabolite in human plasma has been established. Clozapine and N-desmethylclozapine were extracted with n-hexane-isoamyl alcohol (98.5:1.5, v/v). Protriptyline served as the internal standard. The limits of detection for clozapine and desmethylclozapine are 2 and 1 ng/ml, respectively. The sensitivity and precision of this method can be utilized for pharmacokinetic studies and therapeutic drug monitoring regimens.
authors.affiliation: Taipei City Psychiatric Centre, Taiwan.
authors.forename: M C
authors.initials: MC
authors.lastname: Chung
authors.forename: S K
authors.initials: SK
authors.lastname: Lin
authors.forename: W H
authors.initials: WH
authors.lastname: Chang
authors.forename: M W
authors.initials: MW
authors.lastname: Jann
mesh_terms: D000328:Adult; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003024:Clozapine / Q000031:analogs & derivatives* / Q000097:blood*; D006801:Humans; D008297:Male; D012559:Schizophrenia / Q000097:blood; D013056:Spectrophotometry, Ultraviolet
chemical_list: C058272:norclozapine; D003024:Clozapine