Regulation of vasopressin gene expression: changes in the level, but not the size, of vasopressin mRNA following endocrine manipulations.
1. Regulatory interactions between the hypothalamoneurohypophyseal vasopressin (VP) axis and the endocrine systems of the anterior pituitary have been investigated in the rat by observing changes in VP mRNA expression following endocrine manipulations. 2. An increase in the level, but not size, of VP mRNA was found in the supraoptic (SON) and paraventricular nuclei (PVN) of the hypothalamus and in the neurointermediate lobe (NIL) of the pituitary following hypothyroidism (induced by drinking 6-n-propyl-2-thiouracil; PTU) and adrenalectomy. Hypothyroidism induced by alternative procedures (surgical thyroidectomy or PTU injections) did not exert similar effects. 3. Treatment with the dopamine agonist bromocriptine to reduce prolactin secretion raised levels of VP mRNA in the NIL only. Castration did not up-regulate VP mRNA levels. 4. Since the observed effects on VP mRNA levels occur in the absence of changes in plasma osmolality, these results provide evidence of nonosmotic regulation of VP gene expression, an effect which is observed most clearly in the NIL pool of VP mRNA. Furthermore, the effects are distinct from changes in VP mRNA levels associated with raised plasma osmolality since the VP mRNA size was not increased.
Carter
D A
DA
Neuropeptide Laboratory, National University of Singapore.
Pardy
K
K
Murphy
D
D
D000315:Adrenalectomy; D000818:Animals; D001971:Bromocriptine / Q000494:pharmacology; D005786:Gene Expression Regulation; D007030:Hypothalamo-Hypophyseal System / Q000187:drug effects* / Q000378:metabolism; D007037:Hypothyroidism / Q000139:chemically induced / Q000378:metabolism*; D008297:Male; D009919:Orchiectomy; D009994:Osmolar Concentration; D011441:Propylthiouracil / Q000633:toxicity; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013965:Thyroidectomy; D014667:Vasopressins / Q000096:biosynthesis* / Q000235:genetics
D012333:RNA, Messenger; D014667:Vasopressins; D001971:Bromocriptine; D011441:Propylthiouracil