Autoradiographic localization of binding sites for neuropeptide Y and bradykinin on astrocytes.
The cellular localization of binding sites for the vasoactive peptides 125I-neuropeptide Y (NPY) and 3H-bradykinin (BK) was studied in explant cultures of rat cerebellum, brain stem and spinal cord by means of autoradiography. The majority of astrocytes in these cultures expressed binding of 125I-NPY and 3H-BK over their cell bodies and processes. Simultaneous staining of the cultures with anti-glial fibrillary acidic protein (GFAP) has shown that the labelled cells were GFAP-positive and could therefore be identified as astrocytes. In addition to glial cells, a great number of neurones also revealed binding sites for the neuropeptides. Our autoradiographic findings together with recent electrophysiological studies provide good evidence for the existence of NPY- and BK-receptors on astrocytes.
Hösli
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E
Department of Physiology, University of Basel, Switzerland.
Hösli
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L
D000818:Animals; D000831:Animals, Newborn; D001253:Astrocytes / Q000166:cytology / Q000378:metabolism*; D001345:Autoradiography / Q000379:methods; D001933:Brain Stem / Q000378:metabolism*; D002531:Cerebellum / Q000378:metabolism*; D005333:Fetus; D007457:Iodine Radioisotopes; D009478:Neuropeptide Y / Q000378:metabolism; D009924:Organ Culture Techniques; D011689:Purkinje Cells / Q000166:cytology / Q000378:metabolism; D051381:Rats; D017476:Receptors, Neuropeptide Y / Q000032:analysis / Q000378:metabolism*; D013116:Spinal Cord / Q000378:metabolism*; D014316:Tritium
D007457:Iodine Radioisotopes; D009478:Neuropeptide Y; D017476:Receptors, Neuropeptide Y; D014316:Tritium