title: The influence of cell co-operation, nutrients and surface area on cell division.
abstract: Two opposite views have been proposed to explain the decline of the growth potential in cell populations with a limited life span: 1 variations in the probability of cycling and in cycling times or 2 a progressive increase in the nondividing cell fraction. Human brain-derived cells were studied with respect to their proliferative potential under the influence of different growth conditions, using haptotactic palladium islands on agarose. The results emphasize the need for cell co-operation, surface area and nutrients for cell division. These parameters also influence the final cell density. The results illustrate the multiple factors that can vary the probability of initiating the division cycle and stress the uncertainty of defining the irreversible non-dividing state.
authors.affiliation: Department of Oncology, Uppsala University, Sweden.
authors.forename: E
authors.initials: E
authors.lastname: Blomquist
authors.forename: U
authors.initials: U
authors.lastname: Brunk
authors.forename: A
authors.initials: A
authors.lastname: Macieira-Coelho
mesh_terms: D002450:Cell Communication; D002452:Cell Count / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line / Q000187:drug effects; D003470:Culture Media / Q000494:pharmacology*; D005290:Ferric Compounds / Q000494:pharmacology; D006801:Humans; D010165:Palladium
chemical_list: D003470:Culture Media; D005290:Ferric Compounds; D010165:Palladium