Aldose reductase is involved in long-term adaptation of EUE cells to hyperosmotic stress.
Aldose reductase has been shown to be expressed in large amount by human embryonic epithelial cells (EUE) in response to osmotic stress. This conclusion is the result of studies undertaken following the purification to homogeneity of two forms of a 35-kDa protein overexpressed in EUE cells grown in hypertonic saline culture medium as compared to EUE cells grown in isoosmotic medium. Amino-acid composition, molecular weight and partial internal amino-acid sequence showed that the above proteins are two different forms of aldose reductase. These findings were confirmed by the observation that aldose reductase activity increased about 150-fold in adapted cells and returned to basal levels in de-adapted cells.
Ferraretto
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A
Istituto di Fisiologia Veterinaria e Biochimica, Università di Milano, Italy.
Negri
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Giuliani
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De Grada
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Fuhrman Conti
A M
AM
Ronchi
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S
D000222:Adaptation, Physiological; D000449:Aldehyde Reductase / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D002460:Cell Line / Q000201:enzymology; D006801:Humans; D008969:Molecular Sequence Data; D009995:Osmosis; D012462:Saline Solution, Hypertonic
D000596:Amino Acids; D012462:Saline Solution, Hypertonic; D000449:Aldehyde Reductase