title: In vitro effect of indomethacin on polymorphonuclear leukocyte function in preterm infants.
abstract: Random motility and chemotaxis of polymorphonuclear leukocytes (PMN) was evaluated after in vitro exposure to 0, 300, 600, and 900 ng/mL (0.84, 1.68, and 2.52 mumol/L) of indomethacin. PMN were obtained from cord blood of 22 preterm infants of less than 37 wk gestation. For comparison, PMN were obtained from cord blood of seven healthy full-term infants and from venous blood of 10 normal adults. In preterm infants, a significant decrease of random motility and chemotaxis of PMN was noted at all three drug concentrations; impairment of PMN function was dose dependent in the three groups (p < 0.0001), with the greatest effect seen at 900 ng/mL (2.52 mumol/L). Significant impairment of random motility was noted in full-term infants when compared with adults at all indomethacin concentrations and in chemotaxis at 300 and 600 ng/mL (0.84 and 1.68 mumol/L). The study indicates that indomethacin has an adverse effect on PMN random motility and chemotaxis, which is more pronounced in preterm infants.
authors.affiliation: Department of Pediatrics, North Shore University Hospital-Cornell University Medical College, Manhasset, New York 11030.
authors.forename: S
authors.initials: S
authors.lastname: Kamran
authors.forename: S S
authors.initials: SS
authors.lastname: Usmani
authors.forename: R A
authors.initials: RA
authors.lastname: Wapnir
authors.forename: R
authors.initials: R
authors.lastname: Mehta
authors.forename: R G
authors.initials: RG
authors.lastname: Harper
mesh_terms: D000328:Adult; D002465:Cell Movement / Q000187:drug effects; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D005260:Female; D005312:Fetal Blood / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D066298:In Vitro Techniques; D007213:Indomethacin / Q000009:adverse effects*; D007231:Infant, Newborn; D007234:Infant, Premature; D008297:Male; D009504:Neutrophils / Q000187:drug effects* / Q000502:physiology
chemical_list: D007213:Indomethacin