Characterization of cardiac fatty-acid-binding protein from human placenta. Comparison with placenta hepatic types.
When a 105,000 x g supernatant of human placenta was incubated with [1-14C]oleate and subjected to Sephadex G-75 gel filtration and HPLC, two fatty-acid-binding protein (FABP) peaks were obtained. One of these, when further purified by carboxymethyl-cellulose, gave one 15.3-kDa FABP with pI5.3. The other, when chromatographed on DEAE cellulose, separated into two 14.2-kDa FABP with pI6.9 and 5.4. Purity of the proteins was checked by SDS/PAGE. Molecular mass, pI, immunochemical properties and amino acid compositions all indicated that 15.3-kDa FABP was of the cardiac type, whereas both 14.2-kDa FABP were of the hepatic type. Cardiac FABP did not cross-react with hepatic proteins. When tested for the acceptor/donor properties of these FABP, hepatic types were found to be better candidates than cardiac in uptaking fatty acids from liposomes. Cardiac FABP, on the other hand, acted in a more efficient way as a donor, indicating a distinct role of these proteins in human placenta, which furnishes a multiorgan system for the developing fetus.
Das
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Department of Biochemistry, University of Calcutta, India.
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Mukherjea
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D000596:Amino Acids / Q000032:analysis; D002352:Carrier Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D003600:Cytosol / Q000737:chemistry; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D005260:Female; D006801:Humans; D005779:Immunodiffusion; D007526:Isoelectric Point; D008055:Lipids / Q000032:analysis; D008081:Liposomes / Q000378:metabolism; D008099:Liver / Q000737:chemistry*; D008970:Molecular Weight; D009206:Myocardium / Q000737:chemistry*; D009363:Neoplasm Proteins; D019301:Oleic Acid; D009829:Oleic Acids / Q000378:metabolism; D010920:Placenta / Q000737:chemistry*; D011247:Pregnancy; D013229:Stearic Acids / Q000378:metabolism; D025521:Tumor Suppressor Proteins
D000596:Amino Acids; D002352:Carrier Proteins; C107608:FABP7 protein, human; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D008055:Lipids; D008081:Liposomes; D009363:Neoplasm Proteins; D009829:Oleic Acids; D013229:Stearic Acids; D025521:Tumor Suppressor Proteins; D019301:Oleic Acid; C031183:stearic acid