title: Hair cell regeneration in the bullfrog vestibular otolith organs following aminoglycoside toxicity.
abstract: Adult bullfrog were given single intraotic injections of the aminoglycoside antibiotic gentamicin sulfate and sacrificed at postinjection times ranging from 0.5 to 9 days. The saccular and utricular maculae of normal and injected animals were examined in wholemount and cross-section. Intraotic 200 microM gentamicin concentrations resulted in the uniform destruction of the hair bundles and, at later times, the cell bodies of saccular hair cells. In the utriculus, striolar hair cells were selectively damaged while extrastriolar hair cells were relatively unaffected. Regenerating hair cells, identified in sectioned material by their small cell bodies and short, well-formed hair bundles, were seen in the saccular and utricular maculae as early as 24-48 h postinjection. Immature versions of mature hair cell types in both otolith organs were recognized by the presence or absence of a bulbed kinocilia and the relative lengths of their kinocilia and longest stereocilia. Utricular hair cell types with kinocilia longer than their longest stereocilia were observed at earlier than hair cell types with shorter kinocilia. In the sacculus, the hair bundles of gentamicin-treated animals, even at 9 days postinjection, were significantly smaller than those of normal animals. The hair bundles of utricular hair cells, on the other hand, reached full maturity within the same time period.
authors.affiliation: Good Samaritan Hospital and Medical Center, R.S. Dow Neurological Sciences Institute, Portland, OR 97209.
authors.forename: R A
authors.initials: RA
authors.lastname: Baird
authors.forename: M A
authors.initials: MA
authors.lastname: Torres
authors.forename: N R
authors.initials: NR
authors.lastname: Schuff
mesh_terms: D000818:Animals; D002452:Cell Count; D002923:Cilia / Q000187:drug effects / Q000648:ultrastructure; D005260:Female; D005839:Gentamicins / Q000633:toxicity*; D006198:Hair Cells, Auditory / Q000166:cytology / Q000187:drug effects*; D008297:Male; D011892:Rana catesbeiana; D012444:Saccule and Utricle / Q000166:cytology / Q000187:drug effects*
chemical_list: D005839:Gentamicins