Structure of the rat aromatic L-amino acid decarboxylase gene: evidence for an alternative promoter usage.
Aromatic L-amino acid decarboxylase catalyzes the biosynthesis of the neurotransmitters dopamine and serotonin. This enzyme is also expressed in nonneuronal tissues. Two reported cDNA sequences show that the pheochromocytoma message differs from the liver message only at the 5' untranslated region. We present the complete exonal organization and promoter sequences of the rat gene encoding this enzyme. The rat aromatic L-amino acid decarboxylase gene is composed of two promoters and 16 exons spanning more than 80 kb in the genome. The first exon carries the majority of the 5' untranslated sequence of the liver cDNA, and the second exon carries that of the pheochromocytoma cDNA. In the third exon, there are two alternatively utilized splicing acceptors specific to the first and second exons. Therefore, both alternative promoter usage and alternative splicing are operative for the differential expression of this gene. The sequence of each promoter region shows putative binding sites for octamer factors and AP-2.
Hahn
S L
SL
Laboratory of Molecular Neurobiology, Burke Medical Research Institute, Cornell University Medical College, White Plains, New York 10605.
Hahn
M
M
Kang
U J
UJ
Joh
T H
TH
D000818:Animals; D001142:Aromatic-L-Amino-Acid Decarboxylases / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D003360:Cosmids; D005091:Exons; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D051381:Rats; D014018:Tissue Distribution
D001142:Aromatic-L-Amino-Acid Decarboxylases