title: Alcohol intake increases human immunodeficiency virus type 1 replication in human peripheral blood mononuclear cells.
abstract: The effects of alcohol consumption on various T lymphocyte subset functions and on the degree of susceptibility of peripheral blood mononuclear cells (PBMC) to human immunodeficiency virus (HIV) type 1 infection and replication in vitro were investigated. PBMC from 60 HIV-1-seronegative healthy volunteers were studied before and after ingestion of alcohol beverages. After alcohol consumption, there was significantly increased HIV-1 replication (P < .001) in PBMC, as determined by HIV-1 p24 antigen levels in the culture supernatants, than in cultures obtained before alcohol ingestion. There was a decreased ability of lymphocytes, obtained after alcohol consumption, to produce interleukin-2 and soluble immune response suppressor activity after stimulation with concanavalin A. The data show that alcohol ingestion increases HIV-1 replication in human PBMC infected with HIV-1 in cell culture. This may be due to alcohol-induced functional impairment of various subsets of lymphocytes in the peripheral blood. Thus, HIV-1 replication may be augmented by alcohol in HIV-1-infected individuals, and alcohol intake may increase an individual's risk for acquiring HIV-1 infection.
authors.affiliation: Dorrance H. Hamilton Laboratories, Department of Medicine, Jefferson Medical College, Thomas Jefferson University, Philadelphia, PA 19107.
authors.forename: O
authors.initials: O
authors.lastname: Bagasra
authors.forename: A
authors.initials: A
authors.lastname: Kajdacsy-Balla
authors.forename: H W
authors.initials: HW
authors.lastname: Lischner
authors.forename: R J
authors.initials: RJ
authors.lastname: Pomerantz
mesh_terms: D000293:Adolescent; D000328:Adult; D000428:Alcohol Drinking; D000434:Alcoholic Beverages; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D005260:Female; D016655:HIV Core Protein p24 / Q000032:analysis; D015497:HIV-1 / Q000502:physiology*; D006801:Humans; D017403:In Situ Hybridization; D007376:Interleukin-2 / Q000096:biosynthesis; D008297:Male; D008875:Middle Aged; D016176:T-Lymphocyte Subsets / Q000382:microbiology; D013601:T-Lymphocytes / Q000187:drug effects / Q000378:metabolism / Q000382:microbiology* / Q000502:physiology; D013997:Time Factors; D014779:Virus Replication
chemical_list: D016655:HIV Core Protein p24; D007376:Interleukin-2; D003208:Concanavalin A