title: Effects of different enzymic treatments on the release of titin fragments from rabbit skeletal myofibrils. Purification of an 800 kDa titin polypeptide.
abstract: In myofibrils, titin (also called connectin) molecules span from Z line to M line and constitute a third filament system containing an elastic domain in the I band. This giant protein is particularly sensitive to proteolysis in situ. Treatment of rabbit skeletal myofibrils with exogenous proteinases induces a release of titin fragments, which are detected in the soluble myofibrillar fraction. The cleavage of titin occurs at specific points localized at the proximity of Z line and could lead to a concomitant release of alpha-actinin.
authors.affiliation: Centre de Recherches de Biochimie Macromoléculaire LP 9008 (C.N.R.S.), Université Montpellier I, France.
authors.forename: C
authors.initials: C
authors.lastname: Astier
authors.forename: J P
authors.initials: JP
authors.lastname: Labbé
authors.forename: C
authors.initials: C
authors.lastname: Roustan
authors.forename: Y
authors.initials: Y
authors.lastname: Benyamin
mesh_terms: D000818:Animals; D015153:Blotting, Western; D064211:Connectin; D010450:Endopeptidases / Q000378:metabolism*; D015151:Immunoblotting; D008970:Molecular Weight; D009124:Muscle Proteins / Q000302:isolation & purification / Q000378:metabolism*; D009210:Myofibrils / Q000378:metabolism*; D010446:Peptide Fragments / Q000302:isolation & purification / Q000378:metabolism*; D011494:Protein Kinases; D011817:Rabbits; D012697:Serine Endopeptidases / Q000378:metabolism; D014108:Tosylphenylalanyl Chloromethyl Ketone / Q000494:pharmacology; D014357:Trypsin / Q000378:metabolism
chemical_list: D064211:Connectin; D009124:Muscle Proteins; D010446:Peptide Fragments; D014108:Tosylphenylalanyl Chloromethyl Ketone; D011494:Protein Kinases; D010450:Endopeptidases; C044499:submandibular proteinase A; D012697:Serine Endopeptidases; C028344:glutamyl endopeptidase; D014357:Trypsin