Are antibodies reactive with chondrocyte proteins enriched in synovial fluids of patients with rheumatoid arthritis?
In this study we examined the reactivity of synovial fluid (SF) antibodies (Abs) from patients with rheumatoid arthritis (RA) against chondrocyte proteins. The levels of IgG, IgM and IgA in the synovial fluids varied from patient to patient, but were similar to the levels found in each individual's serum. Although the Abs in SF and serum of RA patients reacted with several molecular weight classes of chondrocyte proteins, this reactivity was very low. Synovial fluid and serum Abs from the same patients reacted with similar chondrocyte proteins and to a similar extent, suggesting that the level of Abs reactive with chondrocyte proteins is not enriched in the SF. These data demonstrate that SF from RA patients contain high levels of Abs, however, there is very little reactivity with chondrocyte proteins beyond that observed in serum of normal and RA patients.
Hua
F
F
Samuel Lunenfeld Research Institute, Dept. of Clinical Biochemistry, Mount Sinai Hospital, Toronto, Canada.
Sustackova
H
H
Keystone
E
E
Kandel
R
R
Cruz
T
T
D000818:Animals; D000906:Antibodies / Q000276:immunology*; D001172:Arthritis, Rheumatoid / Q000276:immunology*; D015153:Blotting, Western; D002358:Cartilage, Articular / Q000166:cytology / Q000276:immunology*; D002417:Cattle; D006801:Humans; D007070:Immunoglobulin A / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D008970:Molecular Weight; D011506:Proteins / Q000276:immunology; D013582:Synovial Fluid / Q000276:immunology*
D000906:Antibodies; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D011506:Proteins