Sequence-selective binding of amiloride to DNA.
Nuclease footprinting techniques have been employed to investigate the interaction between the diuretic drug amiloride, a sodium channel blocker with potential therapeutic use in the treatment of cystic fibrosis, and three DNA fragments of defined sequence. Using either DNAse I or micrococcal nuclease as probes, an unusual pattern of sequence-selective recognition of DNA has been detected. Amiloride binds selectively to sites rich in adenine and thymine residues, frequently with an apparent preference for 5'-TpX-3' steps, and discriminates strongly against GC-rich sequences which are sometimes cut more readily in the presence of the drug compared to the control. A detailed comparison with the actions of known selective DNA-binding antibiotics and drugs reveals a unique pattern of binding sites, different from those of typical intercalators on the one hand and those of minor groove-binders on the other. Amiloride is believed to adopt a pH-dependent tricyclic hydrogen-bonded conformation in solution which allows it to intercalate into DNA; consistent with this belief, we find that the footprinting pattern largely disappears at pH values above the pKa. Preliminary studies with three amiloride analogues have indicated the importance of two functional groups in the recognition of DNA. The possible relevance of selective DNA binding to activity in vivo is considered.
Bailly
C
C
Department of Pharmacology, University of Cambridge, U.K.
Cuthbert
A W
AW
Gentle
D
D
Knowles
M R
MR
Waring
M J
MJ
D000584:Amiloride / Q000737:chemistry*; D001483:Base Sequence; D001665:Binding Sites; D004247:DNA / Q000737:chemistry*; D004269:DNA, Bacterial / Q000737:chemistry*; D003850:Deoxyribonuclease I; D004926:Escherichia coli / Q000235:genetics; D007364:Intercalating Agents; D007700:Kinetics; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D010957:Plasmids; D011401:Promoter Regions, Genetic; D012365:RNA, Transfer, Tyr / Q000235:genetics
D004269:DNA, Bacterial; D007364:Intercalating Agents; D009838:Oligodeoxyribonucleotides; D012365:RNA, Transfer, Tyr; C036483:T-DNA; D000584:Amiloride; D004247:DNA; D003850:Deoxyribonuclease I