title: Non-enzymatic glycation of histones.
abstract: 2-(2-Furoyl)-5-(2-furanyl)-1H-imidazole (FFI) is detected in total histones and the lysine-rich histone 1 fraction isolated from calf thymus after in vitro glycation and hydrolysis with HCl, using gas chromatographic/mass spectrometric analysis. The finding suggests that glucose is bound to the epsilon-amino groups of the lysine residues of the histones. No FFI is monitored in native histone samples.
authors.affiliation: Medizinische Universitätsklinik, Tübingen, Germany.
authors.forename: H M
authors.initials: HM
authors.lastname: Liebich
authors.forename: E
authors.initials: E
authors.lastname: Gesele
authors.forename: C
authors.initials: C
authors.lastname: Wirth
authors.forename: J
authors.initials: J
authors.lastname: Wöll
authors.forename: K
authors.initials: K
authors.lastname: Jobst
authors.forename: A
authors.initials: A
authors.lastname: Lakatos
mesh_terms: D000818:Animals; D002417:Cattle; D008401:Gas Chromatography-Mass Spectrometry; D005947:Glucose / Q000737:chemistry*; D006657:Histones / Q000737:chemistry* / Q000302:isolation & purification; D006868:Hydrolysis; D007093:Imidazoles; D007202:Indicators and Reagents; D010084:Oxidation-Reduction; D013950:Thymus Gland / Q000737:chemistry
chemical_list: D006657:Histones; D007093:Imidazoles; D007202:Indicators and Reagents; C041306:2-(2-furoyl)-4(5)-(2-furanyl)-1H-imidazole; D005947:Glucose