title: Characterization of the promoter from the single-copy gene encoding ferredoxin-NADP(+)-oxidoreductase from spinach.
abstract: We describe a genomic DNA segment from spinach that bears part of the single-copy gene for ferredoxin-NADP(+)-oxidoreductase (FNR) including a 3.4 kb promoter sequence. Dissection of this DNA segment and its analysis in GUS (beta-glucuronidase) gene fusions in transgenic tobacco demonstrated that the promoter differs in structure from all other promoters for thylakoid protein genes studied to date. Two regions with light-responsive elements were identified. One is located within the first 118 bp upstream of the transcription initiation site. A second fragment covering nucleotide positions -220 to -119 is capable of conferring light-dependent GUS gene expression on two different minimal promoters. The latter fragment binds a transacting factor in gel-shift assays. None of the fragments carries cis elements known from other genes to be involved in light-controlled expression. Comparison of the light responsiveness of GUS gene fusions controlled by the -753/+231 and -118/+231 regions indicates that they respond differentially to phytochrome-dependent signals and that their expression in tobacco is not restricted to tissue with functional chloroplasts.
authors.affiliation: Botanisches Institut, Ludwig-Maximilians-Universität, München, FRG.
authors.forename: R
authors.initials: R
authors.lastname: Oelmüller
authors.forename: C
authors.initials: C
authors.lastname: Bolle
authors.forename: A K
authors.initials: AK
authors.lastname: Tyagi
authors.forename: N
authors.initials: N
authors.lastname: Niekrawietz
authors.forename: S
authors.initials: S
authors.lastname: Breit
authors.forename: R G
authors.initials: RG
authors.lastname: Herrmann
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004252:DNA Mutational Analysis; D005287:Ferredoxin-NADP Reductase / Q000235:genetics*; D005786:Gene Expression Regulation / Q000528:radiation effects; D017343:Genes, Plant / Q000235:genetics*; D005966:Glucuronidase / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification; D006651:Histocytochemistry; D008027:Light; D008969:Molecular Sequence Data; D045322:Photosynthetic Reaction Center Complex Proteins / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics; D010945:Plants, Edible / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000235:genetics; D010947:Plants, Toxic; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D011994:Recombinant Proteins / Q000096:biosynthesis; D012689:Sequence Homology, Nucleic Acid; D014026:Nicotiana / Q000235:genetics
chemical_list: D045322:Photosynthetic Reaction Center Complex Proteins; D010940:Plant Proteins; D012333:RNA, Messenger; D011994:Recombinant Proteins; D005287:Ferredoxin-NADP Reductase; D005966:Glucuronidase