Evaluation of several affinity chromatographic supports for the purification of maltose-binding protein from Escherichia coli.
To obtain affinity adsorbents with good mechanical resistance, suitable for the purification of maltose-binding protein (MBP) from Escherichia coli and genetically engineered proteins fused to MBP, a series of supports were prepared by grafting amylose on to agarose by different chemistries. Their capacities for MBP and their abilities to be used at relatively high flow-rates were examined. Efficient supports were most conveniently prepared by coupling amylose to epoxy-activated agarose in an aqueous-organic mixture.
Kroviarski
Y
Y
INSERM U 76, Paris, France.
Cochet
S
S
Martineau
P
P
Cartron
J P
JP
Bertrand
O
O
D018528:ATP-Binding Cassette Transporters; D000688:Amylose / Q000737:chemistry; D002352:Carrier Proteins / Q000302:isolation & purification*; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002846:Chromatography, Affinity / Q000379:methods*; D004852:Epoxy Compounds / Q000737:chemistry; D004926:Escherichia coli / Q000737:chemistry*; D029968:Escherichia coli Proteins; D005069:Evaluation Studies as Topic; D007202:Indicators and Reagents; D058619:Maltose-Binding Proteins; D009004:Monosaccharide Transport Proteins; D011993:Recombinant Fusion Proteins / Q000302:isolation & purification; D012685:Sepharose / Q000737:chemistry
D018528:ATP-Binding Cassette Transporters; D002352:Carrier Proteins; D004852:Epoxy Compounds; D029968:Escherichia coli Proteins; D007202:Indicators and Reagents; D058619:Maltose-Binding Proteins; D009004:Monosaccharide Transport Proteins; D011993:Recombinant Fusion Proteins; C461599:maltose transport system, E coli; D000688:Amylose; D012685:Sepharose