Circulating levels of active, total and inactive renin (prorenin), angiotensin I and angiotensinogen in carbon tetrachloride-treated rats.
1. Plasma renin activity (PRA), plasma angiotensin I concentration (ANG I), plasma angiotensinogen concentration (PAC) and the plasma levels of active, total and inactive renin (prorenin) were measured in rats with carbon tetrachloride (CCl4)-induced acute renal failure. Rats were treated with a single oral dose of CCl4 (2.5 mL/kg) and killed 1, 2, 3 and 7 days later. 2. On days 1-3 PRA, ANG I and PAC decreased and increased on day 7. Active renin fell on days 2 and 3, total renin (trypsin treatment) augmented on day 1 and diminished on day 3, prorenin and per cent prorenin increased on days 1 and 2. Angiotensin I concentration paralleled PRA and PAC. The CCl4-induced decrease in PRA was secondary to the fall in active renin and in PAC. Total renin augmented as a consequence of the elevation of prorenin. Renal function, evaluated by serum urea, serum creatinine and creatinine clearance, decreased on days 1 and 2 when PRA was low and plasma prorenin was high. 3. These data do not support the involvement of the circulating active renin-angiotensin system (RAS) in the pathophysiology of acute renal failure induced by CCl4, however, increased prorenin levels were associated with the decrease in renal function.
Cruz
C
C
Department of Nephrology and Mineral Metabolism, Instituto Nacional de la Nutrición Salvador Zubirán, México, D.F.
Ibarra-Rubio
M E
ME
Pedraza-Chaverrí
J
J
D058186:Acute Kidney Injury / Q000097:blood* / Q000139:chemically induced / Q000201:enzymology; D000803:Angiotensin I / Q000097:blood*; D000808:Angiotensinogen / Q000097:blood*; D000818:Animals; D002251:Carbon Tetrachloride; D004792:Enzyme Precursors / Q000097:blood; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012083:Renin / Q000097:blood*
D004792:Enzyme Precursors; D000808:Angiotensinogen; D000803:Angiotensin I; D002251:Carbon Tetrachloride; D012083:Renin