title: Determination of the myosin step size from mechanical and kinetic data.
abstract: During muscle contraction, work is generated when a myosin cross-bridge attaches to an actin filament and exerts a force on it through some power-stroke distance, h. At the end of this power stroke, attached myosin heads are carried into regions where they exert a negative force on the actin filament (the drag stroke) and where they are released rapidly from actin by ATP binding. Although the length of the power stroke remains controversial, average distance traversed in the drag-stroke region can be determined when one knows both rate of cross-bridge dissociation and filament-sliding velocity. At maximum contraction velocity, the average force exerted in the drag stroke must balance that exerted in the power stroke. We discuss here a simple model of cross-bridge interaction that allows one to calculate the force exerted in the drag stroke and to relate this to the power-stroke distance h traversed by cross-bridges in the positive-force region. Both the rate at which myosin can be dissociated from actin and the velocity at which an actin filament can be translated have been measured for a series of myosin isozymes and for different substrates, producing a wide range of values for each. Nonetheless, we show here that the rate of myosin dissociation from actin correlates well with the velocity of filament sliding, providing support for the simple model presented and suggesting that the power stroke is approximately 10 nm in length.
authors.affiliation: Department of Pure and Applied Mathematics, Washington State University, Pullman 99164.
authors.forename: E
authors.initials: E
authors.lastname: Pate
authors.forename: H
authors.initials: H
authors.lastname: White
authors.forename: R
authors.initials: R
authors.lastname: Cooke
mesh_terms: D000199:Actins / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D007700:Kinetics; D008433:Mathematics; D008954:Models, Biological; D009132:Muscles / Q000378:metabolism*; D009218:Myosins / Q000737:chemistry* / Q000378:metabolism*; D011817:Rabbits; D011898:Ranidae; D013816:Thermodynamics
chemical_list: D000199:Actins; D000255:Adenosine Triphosphate; D009218:Myosins