title: Expression of histone and alkaline phosphatase genes in UMR 106-01 rat osteoblast-like cells exposed to the Hoechst dye H33342.
abstract: The fluorescent Dye H33342 (H342) is a bis-benzimidazole used for intravital fluorescent staining. In this report, we found that H342 completely abolished histone 2a mRNA but had no effect on alkaline phosphatase gene expression and protein synthesis in UMR 106-01 rat osteoblast-like cells. The complete loss of histone 2a mRNA occurred after only 20 min of treatment with H342. This effect is unlikely to be a result of inhibition of DNA synthesis, which was only partly suppressed. The mechanism of the action of H342 on histone 2a mRNA is presently unknown.
authors.affiliation: Department of Orthopaedic Surgery, University of Western Australia, Nedlands.
authors.forename: M H
authors.initials: MH
authors.lastname: Zheng
authors.forename: S
authors.initials: S
authors.lastname: Wysocki
authors.forename: J M
authors.initials: JM
authors.lastname: Papadimitriou
authors.forename: D
authors.initials: D
authors.lastname: Wood
mesh_terms: D000469:Alkaline Phosphatase / Q000187:drug effects* / Q000235:genetics; D000818:Animals; D001562:Benzimidazoles / Q000494:pharmacology*; D004247:DNA / Q000096:biosynthesis / Q000187:drug effects; D005456:Fluorescent Dyes / Q000494:pharmacology*; D015870:Gene Expression / Q000187:drug effects; D006657:Histones / Q000187:drug effects* / Q000235:genetics; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects*; D012516:Osteosarcoma; D014176:Protein Biosynthesis; D011506:Proteins / Q000187:drug effects; D012333:RNA, Messenger / Q000187:drug effects; D051381:Rats; D014407:Tumor Cells, Cultured
chemical_list: D001562:Benzimidazoles; D005456:Fluorescent Dyes; D006657:Histones; D011506:Proteins; D012333:RNA, Messenger; D004247:DNA; D000469:Alkaline Phosphatase; C017807:bisbenzimide ethoxide trihydrochloride