An estrogen-dependent glycoprotein is synthesized and released from the oviduct in a temporal- and region-specific manner during early pregnancy in the ewe.
Administration of estradiol-17 beta (E) to ovariectomized (ovx) sheep results in the synthesis and release of an M(r) 90,000-92,000 glycoprotein into the oviductal lumen and into culture medium of ampullar explants (Biol Reprod 1992; 47:889-902). The objective of this study was to determine when and from what region of the oviduct the M(r) 90,000-92,000 glycoprotein is synthesized and released during early pregnancy. Estrous ewes were bred to intact rams of known fertility, and oviducts were obtained at estrus (Day 0) and at Days 1.5, 2, 3, 4, 6, and 16 of pregnancy. Pregnancy was verified by the presence of a fertilized egg or developing conceptus and a functional corpus luteum. Oviductal secretions were collected by flushing oviducts with saline and by explant culture. The oviductal fimbria, ampulla, and isthmus were individually cultured (24 h) in the presence of 3H-leucine (3H-leu) or 3H-glucosamine (3H-glcN). The presence of the M(r) 90,000-92,000 glycoprotein in oviductal flushings and culture medium was determined by fluorography and Western blotting. The M(r) 90,000-92,000 protein was present in SDS gels and blots of oviductal flushings from animals through Days 4-6 of pregnancy, but not in flushings from Day 16 pregnant animals or from ovx, untreated animals. This protein was present in 3H-leu- and 3H-glcN-labeled culture medium of the oviductal ampulla (Days 0, 1.5, 2, 3, 4, 6, and 16) and fimbria (Days 0, 1.5, 2, 3, and 4) during early pregnancy.(ABSTRACT TRUNCATED AT 250 WORDS)
Murray
M K
MK
Department of Anatomy and Cellular Biology, Tufts University School of Veterinary Medicine, Boston, Massachusetts 02111.
D000818:Animals; D004958:Estradiol / Q000494:pharmacology; D005187:Fallopian Tubes / Q000187:drug effects / Q000378:metabolism*; D005260:Female; D006023:Glycoproteins / Q000096:biosynthesis* / Q000737:chemistry / Q000378:metabolism; D066298:In Vitro Techniques; D008970:Molecular Weight; D011247:Pregnancy; D012756:Sheep; D013997:Time Factors; D014018:Tissue Distribution
D006023:Glycoproteins; D004958:Estradiol