title: Antibody development against northern fowl mites (Acari: Macronyssidae) in chickens.
abstract: This study reports the development of an antibody against protein(s) from the tissue of the northern fowl mite, Ornithonyssus sylviarum (Canestrini & Fanzago). Northern fowl mite proteins were obtained by affinity chromatography and used for immunization. Western blot analysis identified proteins that were reactive with sera from birds immunized with the antigen; this indicated that serum antibodies against the northern fowl mite had been produced. Chickens that had been immunized or infested, or both, with the northern fowl mite produced sera that were reactive with a 100 kilodalton (kD) protein. The response was greater if the chicken had been immunized with the antigen and infested with the northern fowl mite. Experimentally immunized and infested chickens experienced limited decreases in the levels of northern fowl mite infestation. Survival of bloodfed mites after ingestion of the immune chicken blood was assessed in an in vitro feeding study using blood-filled parafilm sacs; minor differences in northern fowl mite feeding tendencies were noted. The chickens developed antibodies to the northern fowl mite proteins, but this immunity did not decrease the infestation level or in vitro feeding.
authors.affiliation: USDA-ARS, Livestock Poultry Sciences Institute, Livestock Insects Laboratory, Beltsville, MD 20705-2350.
authors.forename: N M
authors.initials: NM
authors.lastname: Minnifield
authors.forename: J
authors.initials: J
authors.lastname: Carroll
authors.forename: K
authors.initials: K
authors.lastname: Young
authors.forename: D K
authors.initials: DK
authors.lastname: Hayes
mesh_terms: D000818:Animals; D000917:Antibody Formation; D000941:Antigens / Q000276:immunology / Q000302:isolation & purification; D015153:Blotting, Western; D002645:Chickens / Q000276:immunology*; D007114:Immunization / Q000662:veterinary*; D008297:Male; D008924:Mite Infestations / Q000276:immunology / Q000662:veterinary*; D008925:Mites / Q000276:immunology*; D011201:Poultry Diseases / Q000276:immunology*
chemical_list: D000941:Antigens