Expression of human thromboxane synthase using a baculovirus system.
Human thromboxane (TX) synthase (EC 5.3.99.5) was produced by the baculovirus expression system using cDNA encoding human TX synthase [(1991) Biochem. Biophys. Res. Commun. 78, 1479-1484]. A recombinant baculovirus TXS7 was expressed in Spodoptera frugiperda Sf9 insect cells. The expressed protein was recognized by monoclonal antibody, Kon 7 raised against human TX synthase [(1990) Blood 76, 80-85]. The recombinant TX synthase catalyzed the conversion of prostaglandin (PG) H2 to TXA2 and 12-hydroxy-heptadecatrienoic acid (HHT). Both conversions of PGH2 to TXA2 and HHT by the expressed TX synthase were completely inhibited by a specific TX synthase inhibitor, OKY-046 (5 microM).
Yokoyama
C
C
Department of Pharmacology, National Cardiovascular Center Research Institute, Osaka, Japan.
Miyata
A
A
Suzuki
K
K
Nishikawa
Y
Y
Yoshimoto
T
T
Yamamoto
S
S
Nüsing
R
R
Ullrich
V
V
Tanabe
T
T
D000818:Animals; D016367:Baculoviridae; D002384:Catalysis; D002478:Cells, Cultured; D003001:Cloning, Molecular; D004247:DNA; D005231:Fatty Acids, Unsaturated / Q000378:metabolism; D006801:Humans; D009036:Moths; D044262:Prostaglandin H2; D011463:Prostaglandins H / Q000378:metabolism; D013930:Thromboxane-A Synthase / Q000235:genetics* / Q000378:metabolism; D013931:Thromboxanes / Q000378:metabolism
D005231:Fatty Acids, Unsaturated; D011463:Prostaglandins H; D013931:Thromboxanes; D044262:Prostaglandin H2; C008033:12-hydroxy-5,8,10-heptadecatrienoic acid; D004247:DNA; D013930:Thromboxane-A Synthase