Modulation of interferon-gamma-induced HLA-DR expression on the human keratinocyte cell line SCC-13 by ultraviolet radiation.
Cell surface expression of major histocompatibility determinants on epidermal keratinocytes is a characteristic feature of a number of inflammatory dermatoses and in all likelihood is caused by diffusion of human leukocyte antigen (HLA)-DR-inducing cytokines from cells present in the dermal mononuclear cell infiltrate. Many of these same disorders respond to ultraviolet (UV) radiation phototherapy. Using the human SCC-13 keratinocyte cell line as a model, UV radiation was found to inhibit interferon-gamma-induced HLA-DR expression. Inhibition correlated closely with decreased steady-state levels of HLA-DR mRNA. These findings provide evidence that the therapeutic effect of UV radiation phototherapy may be mediated by its capacity to down-regulate cytokine-induced keratinocyte HLA-DR expression.
Khan
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IU
Department of Dermatology, Case Western Reserve University, Cleveland, OH 44106.
Boehm
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KD
Elmets
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CA
D002460:Cell Line; D015232:Dinoprostone / Q000494:pharmacology; D004307:Dose-Response Relationship, Radiation; D005802:Genes, MHC Class II / Q000528:radiation effects; D006684:HLA-DR Antigens / Q000096:biosynthesis / Q000235:genetics*; D006801:Humans; D007213:Indomethacin / Q000494:pharmacology; D007371:Interferon-gamma / Q000494:pharmacology*; D015603:Keratinocytes / Q000276:immunology / Q000528:radiation effects*; D012333:RNA, Messenger / Q000378:metabolism; D011994:Recombinant Proteins; D014466:Ultraviolet Rays
D006684:HLA-DR Antigens; D012333:RNA, Messenger; D011994:Recombinant Proteins; D007371:Interferon-gamma; D015232:Dinoprostone; D007213:Indomethacin