Presence of point mutations in the N-ras gene in radiation-transformed rat embryo cells.
We study the transforming ability of X-rays in multistep carcinogenesis by irradiating primary rat cells which contain transfected c-myc oncogene. X-irradiation induces fully transformed phenotypes, including anchorage-independent growth and tumor formation in nude mice. Of seven foci examined, five exhibited an A to G conversion in codon 61 of the N-ras oncogene. Another transformed isolate has a single G to A base substitution in codon 14 in the same oncogene, while no point mutation is detected in the other focus. This is the first in vitro demonstration of the association between point mutation and X-ray-transformed cells.
Chen
C H
CH
Laboratory of Radiation Biophysics, Memorial Sloan Kettering Cancer Center, New York, New York 10021.
Endlich
B
B
Salavati
R
R
Ling
C C
CC
D016475:3T3 Cells; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002461:Cell Line, Transformed; D003062:Codon / Q000235:genetics / Q000528:radiation effects; D004252:DNA Mutational Analysis / Q000379:methods; D004622:Embryo, Mammalian / Q000528:radiation effects; D005091:Exons / Q000235:genetics / Q000528:radiation effects; D016259:Genes, myc / Q000235:genetics*; D011905:Genes, ras / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D017354:Point Mutation / Q000235:genetics*; D051381:Rats; D014162:Transfection
D003062:Codon