Relationships between age-dependent changes in the effect of almitrine on H(+)-ATPase/ATPsynthase and the pattern of membrane fatty acid composition.
The effects of almitrine on ATPase/ATPsynthase previously described in beef heart mitochondria (Rigoulet et al. (1990) Biochim. Biophys. Acta 1018, 91-97) are also observed in liver mitochondria isolated from rats older than 7 weeks. In contrast, in rats younger than 5 weeks, almitrine at the same concentration has no effect on the ATPase/ATPsynthase complex. This age-dependent action of almitrine is well correlated with age-dependent modifications of two fatty acids: linoleic and docosahexaenoic acids. The possibility of a change in H+/ATP stoichiometry of the ATPase/ATPsynthase induced by almitrine seems related to more general modifications of membrane properties during growth of the rat.
Jumelle-Laclau
M
M
Institut de Biochimie cellulaire et Neurochimie du CNRS, Université de Bordeaux II, France.
Rigoulet
M
M
Averet
N
N
Leverve
X
X
Dubourg
L
L
Carbonneau
A
A
Clerc
M
M
Guerin
B
B
D000375:Aging; D015765:Almitrine / Q000494:pharmacology*; D000818:Animals; D002784:Cholesterol / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis*; D007425:Intracellular Membranes / Q000187:drug effects; D050356:Lipid Metabolism; D008297:Male; D008930:Mitochondria, Liver / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D010758:Phosphorus / Q000032:analysis; D006180:Proton-Translocating ATPases / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
D005227:Fatty Acids; D010758:Phosphorus; D002784:Cholesterol; D015765:Almitrine; D006180:Proton-Translocating ATPases