Cardiovascular responses of conscious rats to carotid body chemoreceptor stimulation by intravenous KCN.
In the present study we analyzed the cardiovascular, respiratory and behavioral responses evoked in rats by KCN. Hypertension, hyperpnea and alerting behavior were characteristic responses in unanesthetized animals. Selective denervation of aortic and carotid bifurcation areas confirmed the carotid body chemoreceptor (CBC) origin of these responses. Sino-aortic denervation, as well as selective carotid sinus denervation abolished the responses, while, after selective aortic denervation, the responses were not different from those of the control rats. The administration of prazosin abolished the hypertensive response but did not change the bradycardic response, whereas the administration of atropine eliminated the bradycardic response and potentiated the hypertensive response to KCN. Increasing doses of KCN (10-80 micrograms/rat) produced proportionally larger bradycardia, hypertension and hyperventilation. Slight attenuation of bradycardic responses to KCN were observed in rats under chloralose anesthesia, while marked depression of bradycardic responses were observed under pentobarbitone anesthesia. Both anesthetics changed the hypertensive responses to hypotensive responses. In conclusion, the cardiovascular, respiratory and behavioral responses to intravenous KCN are a good functional test to CBC stimulation in unanesthetized rats, producing simultaneously intense bradycardia with hyperventilation and behaviour responses.
Franchini
K G
KG
Hypertension Unit, University Hospital, Faculty of Medicine, University of Sao Paulo, Brazil.
Krieger
E M
EM
D000818:Animals; D001794:Blood Pressure / Q000187:drug effects*; D002344:Carotid Body / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D006339:Heart Rate / Q000187:drug effects*; D007275:Injections, Intravenous; D008297:Male; D011190:Potassium Cyanide / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D012119:Respiration / Q000187:drug effects*
D011190:Potassium Cyanide