Kinesin is rapidly transported in the optic nerve as a membrane associated protein.
We have investigated the membrane vs. cytosolic distribution of newly synthesized and total kinesin in rabbit retinal ganglion cell axons which comprise the optic nerve. We find that kinesin is rapidly transported into the axon and that this newly synthesized protein is completely membrane-associated while approximately two third of the total kinesin in the optic nerve is membrane associated. Of this membrane associated kinesin about half is resistant to removal by treatment with 100 mM Na2CO3 (pH 11.3) and none can be stripped by 1 M NaCl. The newly synthesized axonal kinesin is completely resistant to removal by Na2CO3 treatment. By these criteria, at least one third of the total and essentially all of the rapidly transported axonal kinesin appears to exist as an integral membrane protein, consistent with it functioning as the anterograde motor for rapid vesicle transport from the cell body through the axon.
Morin
P J
PJ
Department of Biochemistry, Boston University School of Medicine, MA 02118.
Johnson
R J
RJ
Fine
R E
RE
D000818:Animals; D000906:Antibodies; D001369:Axons / Q000378:metabolism; D002254:Carbonates; D002478:Cells, Cultured / Q000737:chemistry; D002642:Chick Embryo; D003600:Cytosol / Q000378:metabolism; D016547:Kinesins / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism*; D009900:Optic Nerve / Q000378:metabolism*; D011817:Rabbits; D014795:Visual Pathways / Q000378:metabolism
D000906:Antibodies; D002254:Carbonates; D008565:Membrane Proteins; C005686:sodium carbonate; D016547:Kinesins