Short-term culture of exfoliated cells from the urine of patients with bladder tumors.
This report concerns the short-term culture of urothelial cells from the urine sediment of over 100 patients with bladder tumors. Primary cell outgrowth was obtained in approximately 60% of the cultures initiated. Culture outcome was not related to tumor grade, patient age, or volume of the urine sample. Around 85% of the proliferating cultures were successfully transferred into multi-compartment chamber/slides. These results suggest that the culture system may be a useful tool for the study of urothelial cells using patient material obtained by non-invasive means.
Herz
F
F
Department of Pathology, Montefiore Medical Center, Albert Einstein College of Medicine, Bronx, NY 10467.
Deitch
D
D
Adler
S A
SA
Brijlall
D
D
D000368:Aged; D000369:Aged, 80 and over; D002455:Cell Division; D003584:Cytological Techniques; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D014407:Tumor Cells, Cultured / Q000473:pathology*; D001749:Urinary Bladder Neoplasms / Q000473:pathology* / Q000652:urine*; D014556:Urine / Q000166:cytology