title: Retention of antimicrobial activity by human root surfaces after in situ subgingival irrigation with tetracycline HCl or chlorhexidine.
abstract: Substantivity of tetracycline HCl and chlorhexidine digluconate was assessed in extracted teeth. Fifty periodontally compromised teeth scheduled for extraction with probing depths ranging between 6 and 12 mm were root planed and then irrigated in situ with 1 of 4 solutions: tetracycline HCl at concentrations of 10 or 50 mg/ml, 0.12% chlorhexidine digluconate, or 0.9% sterile saline. Each tooth was exposed to 150 ml of the respective irrigation solution. Following extractions, the teeth were transferred to tris buffered saline and incubated at room temperature for 22 days. Incubation solutions were replaced at 24-hour intervals. Removed solutions were examined for desorbed antimicrobial activity using a microtiter assay in which bacterial growth was evaluated by optical density readings. Tetracycline HCl 50 mg/ml exhibited significantly greater antimicrobial activity than chlorhexidine digluconate for 12 days and greater than saline for 16 days. Tetracycline HCl 10 mg/ml exhibited significantly greater antimicrobial activity than chlorhexidine digluconate and saline for 4 days. Chlorhexidine digluconate did not exhibit any significant antimicrobial activity at any time point. Our findings demonstrate long-lasting substantivity of tetracycline HCl, but not chlorhexidine digluconate, by teeth exposed to a single episode of pocket irrigation of their periodontally-exposed roots. The amount of antimicrobial activity retained is proportional to the concentration of tetracycline HCl used for irrigation.
authors.affiliation: Hebew University, Jerusalem, Israel.
authors.forename: A
authors.initials: A
authors.lastname: Stabholz
authors.forename: J
authors.initials: J
authors.lastname: Kettering
authors.forename: R
authors.initials: R
authors.lastname: Aprecio
authors.forename: G
authors.initials: G
authors.lastname: Zimmerman
authors.forename: P J
authors.initials: PJ
authors.lastname: Baker
authors.forename: U M
authors.initials: UM
authors.lastname: Wikesjö
mesh_terms: D000327:Adsorption; D000328:Adult; D000704:Analysis of Variance; D001682:Biological Availability; D002710:Chlorhexidine / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000493:pharmacokinetics / Q000494:pharmacology / Q000627:therapeutic use; D015169:Colony Count, Microbial; D006801:Humans; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D010510:Periodontal Diseases / Q000188:drug therapy* / Q000382:microbiology; D012044:Regression Analysis; D013295:Streptococcus mutans / Q000187:drug effects; D013752:Tetracycline / Q000008:administration & dosage / Q000493:pharmacokinetics* / Q000494:pharmacology / Q000627:therapeutic use; D007507:Therapeutic Irrigation; D013997:Time Factors; D014092:Tooth Root / Q000378:metabolism / Q000382:microbiology*
chemical_list: D013752:Tetracycline; C010882:chlorhexidine gluconate; D002710:Chlorhexidine