title: The hyaluronate synthase from a eukaryotic cell line.
abstract: The hyaluronate synthase complex was identified in plasma membranes from B6 cells. It contained two subunits of molecular masses 52 kDa and 60 kDa which bound the precursor UDP-GlcA in digitonin solution and partitioned into the aqueous phase, together with nascent hyaluronate upon Triton X-114 phase separation. The 52 kDa protein cross-reacted with poly- and monoclonal antibodies raised against the streptococcal hyaluronate synthase and the 60 kDa protein was recognized by monoclonal antibodies raised against a hyaluronate receptor. The 52 kDa protein was purified to homogeneity by affinity chromatography with monoclonal anti-hyaluronate synthase.
authors.affiliation: Institute für Physiologische Chemie und Pathobiochemie, Münster, Germany.
authors.forename: L
authors.initials: L
authors.lastname: Klewes
authors.forename: E A
authors.initials: EA
authors.lastname: Turley
authors.forename: P
authors.initials: P
authors.lastname: Prehm
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D015153:Blotting, Western; D002462:Cell Membrane / Q000201:enzymology*; D002846:Chromatography, Affinity; D006224:Cricetinae; D004797:Enzyme-Linked Immunosorbent Assay; D005944:Glucosamine / Q000031:analogs & derivatives / Q000378:metabolism; D014453:Glucuronosyltransferase / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D016695:Glycosyltransferases; D000076002:Hyaluronan Synthases; D006822:Hybrid Cells; D008168:Lung; D008325:Mammary Neoplasms, Experimental; D008565:Membrane Proteins; D051379:Mice; D008970:Molecular Weight; D014166:Transferases; D014407:Tumor Cells, Cultured; D014537:Uridine Diphosphate N-Acetylglucosamine / Q000378:metabolism; D029867:Xenopus Proteins
chemical_list: D000911:Antibodies, Monoclonal; D008565:Membrane Proteins; D029867:Xenopus Proteins; C026712:UDP-glucosamine; D014537:Uridine Diphosphate N-Acetylglucosamine; D014166:Transferases; D016695:Glycosyltransferases; D014453:Glucuronosyltransferase; C056944:HAS1 protein, Xenopus; D000076002:Hyaluronan Synthases; D005944:Glucosamine