Expression of beta-galactosidase from a hybrid promoter:operator element in Escherichia coli.
A hybrid trpPO:lacO regulatory sequence was cloned upstream of a promoterless lacZ gene and recombined onto a lambda bacteriophage. Escherichia coli lysogens representing the four possible phenotypes for lacI and trpR were constructed and the synthesis of beta-galactosidase was assayed under various growth conditions. The results illustrated that both control elements could be efficiently and independently regulated by the addition or omission of appropriate accessory molecules.
Dixon
K E
KE
Department of Microbiology, Southern Illinois University, Carbondale 62901.
Fix
D F
DF
D003001:Cloning, Molecular; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005798:Genes, Bacterial; D007211:Indoles / Q000494:pharmacology; D007763:Lac Operon; D009875:Operator Regions, Genetic; D011401:Promoter Regions, Genetic; D014364:Tryptophan / Q000494:pharmacology; D001616:beta-Galactosidase / Q000096:biosynthesis*
D007211:Indoles; C001446:indoleacrylic acid; D014364:Tryptophan; D001616:beta-Galactosidase