title: Localization of the virulence-associated genes pilA, pilR, rpoN, fliA, fliC, ent, and fbp on the physical map of Pseudomonas aeruginosa PAO1 by pulsed-field electrophoresis.
abstract: Seven virulence-associated genes have been placed on a genomic map of Pseudomonas aeruginosa PAO1, using pulsed-field electrophoresis, on the basis of the previous physical maps of Romling et al. (U. Romling, M. Duchene, D. Essar, D. Galloway, C. Guidi-Rontani, D. Hill, A. Lazdunski, R. Miller, K. Schleifer, D. Smith, H. Toschka, and B. Tummler, J. Bacteriol. 174:327-330, 1992; U. Romling, D. Grothues, W. Bautsch, and B. Tummler, EMBO J. 8:4081-4089, 1989) and Ratnaningsih et al. (E. Ratnaningsih, S. Dharmsthiti, V. Krishnapillai, A. Morgan, M. Sinclair, and B. W. Holloway, J. Gen. Microbiol. 136:2351-2357, 1990). The new locations for the outer membrane enterobactin iron-siderophore receptor ent gene (41 to 42 min) and the fliA gene (59 to 61 min), which encodes a minor sigma factor of RNA polymerase, are given. The pilA (the pilin structural gene), pilR (a pilin regulatory gene), and rpoN (encoding another minor sigma factor of RNA polymerase) genes map together at 71 to 75 min, locations correcting the previously reported values (V. Shortridge, M. Pato, A. Vasil, and M. Vasil, Infect. Immun. 59:3596-3603, 1990). The fbp gene (28 to 29 min), which encodes an outer membrane ferripyochelin-binding protein of low molecular weight, and the fliC gene (64 to 66 min), the flagellin structural gene, were determined to lie in the previously reported locations.
authors.affiliation: Department of Microbiology, University of Alberta, Edmonton, Canada.
authors.forename: M A
authors.initials: MA
authors.lastname: Farinha
authors.forename: S L
authors.initials: SL
authors.lastname: Ronald
authors.forename: A M
authors.initials: AM
authors.lastname: Kropinski
authors.forename: W
authors.initials: W
authors.lastname: Paranchych
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D036781:Fimbriae Proteins; D005408:Flagellin / Q000235:genetics*; D005798:Genes, Bacterial; D016680:Genome, Bacterial; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D011550:Pseudomonas aeruginosa / Q000235:genetics* / Q000472:pathogenicity; D011956:Receptors, Cell Surface; D015183:Restriction Mapping; D012808:Sigma Factor / Q000235:genetics
chemical_list: D001425:Bacterial Outer Membrane Proteins; D002352:Carrier Proteins; D004269:DNA, Bacterial; D009838:Oligodeoxyribonucleotides; D011956:Receptors, Cell Surface; D012808:Sigma Factor; C029489:enterobactin receptor; D005408:Flagellin; D036781:Fimbriae Proteins