Carbohydrate structures of the cell adhesion molecule, contact site A, from Dictyostelium discoideum.
We determined the carbohydrate structures of contact site A from Dictyostelium discoideum. The carbohydrate moieties of contact site A were released by hydrazinolysis. Fractionation of the deacidified oligosaccharide mixture by Bio-Gel P-4 column chromatography revealed that it was composed of four major oligosaccharides. Their respective structures were determined by sequential exoglycosidase digestion. It is known that contact site A consists of two kinds of carbohydrates, I and II. Taking together the previous and the present results, it was deduced that carbohydrate I comprises N-linked oligosaccharides and carbohydrate II O-linked ones. Furthermore, the relative molar contents of GalNAc and GlcNAc in reducing terminal suggested that contact site A contains 67% of N-linked and 33% of O-linked oligosaccharides.
Yoshida
M
M
Research Institute of Food Science, Kinki University, Higashi-Osaka, Japan.
Matsui
T
T
Fuse
G
G
Ouchi
S
S
D000818:Animals; D001665:Binding Sites; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000737:chemistry*; D015815:Cell Adhesion Molecules / Q000737:chemistry*; D002845:Chromatography; D002851:Chromatography, High Pressure Liquid; D004023:Dictyostelium / Q000737:chemistry*; D006031:Glycosylation; D006834:Hydrazines / Q000378:metabolism; D008361:Mannosidases / Q000378:metabolism; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry / Q000302:isolation & purification; D001616:beta-Galactosidase / Q000378:metabolism; D001619:beta-N-Acetylhexosaminidases / Q000378:metabolism
D002241:Carbohydrates; D015815:Cell Adhesion Molecules; D006834:Hydrazines; D009844:Oligosaccharides; D008361:Mannosidases; D001616:beta-Galactosidase; D001619:beta-N-Acetylhexosaminidases