title: Changes in the expression of Ig-associated proteins on B lymphocytes activated by anti-IgM antibodies.
abstract: Binding of antigen to receptor complexes on B cells elicits a cascade of intracellular signalling events leading to proliferation and, together with T-cell help, Ig secretion. Components of the antigen receptor (AgR) complex have been demonstrated to be either covalently bound or associated with surface Ig (sIg) molecules. The function of these proteins is still unknown. In order to address this question, we have stimulated B cells with anti-mu antibodies and have studied possible changes in the expression of AgR complexes. After anti-mu stimulation, the IgM molecules disappeared rapidly from the cell surface together with the covalently bound proteins. The IgM molecules were internalized and probably degraded. The IgM-associated heterodimer Ig-alpha/Ig-beta was also removed from the cells, leaving the IgD-associated heterodimer unaffected. Two proteins showed an enhanced association with sIg after 15 min and then were gradually removed from the cell surface. Two other proteins became increasingly attached to sIg. This association remained stable for the rest of the culture period (up to 4 h). Further studies are underway to characterize these proteins more closely and to examine possible interactions with downstream members of the signalling cascade.
authors.affiliation: Paul-Ehrlich-Institut, Langen, Germany.
authors.forename: L
authors.initials: L
authors.lastname: Vogel
authors.forename: C
authors.initials: C
authors.lastname: Persin
authors.forename: D
authors.initials: D
authors.lastname: Haustein
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D008213:Lymphocyte Activation; D008222:Lymphokines / Q000378:metabolism*; D051379:Mice; D008815:Mice, Inbred Strains; D029584:Prostatic Secretory Proteins; D011947:Receptors, Antigen, B-Cell / Q000378:metabolism*
chemical_list: D008222:Lymphokines; D029584:Prostatic Secretory Proteins; D011947:Receptors, Antigen, B-Cell; C043076:beta-microseminoprotein; C013059:immunoglobulin-binding factors