Selection of single-stranded deoxyribonucleic acid spermatozoa via the SpermPrep filtration column.
Eighteen semen samples were collected from 18 normospermic men. Two aliquots (1 mL) were prepared from each ejaculate, washed with Ham's F-10, and each washed sperm pellet was reconstituted in 2 mL volume of Ham's F-10 medium. Each aliquot one was stained using the AO-staining method. Each aliquot two was filtered via the SpermPrep II method, and the recovered spermatozoa were stained similarly. The proportion of single-stranded DNA (red) spermatozoa to double-stranded (green) spermatozoa was significantly higher in aliquot one than in the postfiltered sample (aliquot two), suggesting that the SpermPrep filtration procedure selectively entrapped the spermatozoa with abnormal DNA.
Sofikitis
N
N
Tottori University School of Medicine, Yonago, Japan.
Miyagawa
I
I
Zavos
P M
PM
D004277:DNA, Single-Stranded / Q000032:analysis*; D005374:Filtration; D006801:Humans; D008297:Male; D013081:Sperm Motility; D013094:Spermatozoa / Q000737:chemistry*
D004277:DNA, Single-Stranded