title: Urinary excretion of platelet activating factor in patients with immune-mediated glomerulonephritis.
abstract: We have investigated whether human immune-mediated glomerulonephritis is associated with changes in platelet activating factor (PAF) biosynthesis. Urinary PAF, taken as a marker of its renal synthesis, was significantly higher in patients with membranous nephropathy (N = 9) than in healthy controls (N = 8). This was not due to a lower degradation of PAF since urinary acetylhydrolase activity was comparable in patients and controls. A significant positive correlation between urinary excretion of PAF and proteinuria was observed. PAF generation was comparable in polymorphonuclear cells isolated from patients with membranous nephropathy and controls. PAF levels in blood from patients with membranous nephropathy were significantly lower than in controls, suggesting that the excessive generation of PAF is confined to the kidney. The results document that signs of renal disease activity in human membranous nephropathy are associated with an excessive renal synthesis of PAF.
authors.affiliation: Mario Negri Institute for Pharmacological Research, Bergamo, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Noris
authors.forename: A
authors.initials: A
authors.lastname: Benigni
authors.forename: P
authors.initials: P
authors.lastname: Boccardo
authors.forename: E
authors.initials: E
authors.lastname: Gotti
authors.forename: E
authors.initials: E
authors.lastname: Benfenati
authors.forename: S
authors.initials: S
authors.lastname: Aiello
authors.forename: M
authors.initials: M
authors.lastname: Todeschini
authors.forename: G
authors.initials: G
authors.lastname: Remuzzi
mesh_terms: D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase; D000328:Adult; D000368:Aged; D005260:Female; D015433:Glomerulonephritis, Membranous / Q000097:blood / Q000150:complications / Q000652:urine*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000378:metabolism; D010741:Phospholipases A / Q000652:urine; D010972:Platelet Activating Factor / Q000096:biosynthesis / Q000652:urine*; D011507:Proteinuria / Q000209:etiology / Q000652:urine
chemical_list: D010972:Platelet Activating Factor; D010741:Phospholipases A; D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase