Expression of macrophage products after in vitro infection of human monocytes/macrophages with HIV.
We studied the response of monocytes/macrophages (MO/MAC) to lipopolysaccharide (LPS) and interferon-gamma (IFN gamma) stimulation with respect to the expression of macrophage-specific products, i.e. macrophage-colony-stimulating factor (M-CSF), c-fms, c-sis, tissue factors, transforming growth factor-beta (TGF beta) and interleukin-8 (IL8) after in vitro infection with HIV. The expression of IL8 was strongly elevated in HIV-infected cells, peaking at 4 h after stimulation with LPS. At that time, the uninfected control showed only weak expression of IL8. Other products, e.g. tissue factor, c-fms, M-CSF and TGF beta were not modulated after stimulation. In contrast to IL8, the expression of c-cis was significantly lower in infected cells after stimulation with IFN gamma compared to uninfected control cells.
Glienke
W
W
Georg-Speyer-Haus, Chemotherapeutisches Forschungsinstitut, Frankfurt am Main, Germany.
von Briesen
H
H
Esser
R
R
Müller
S
S
Andreesen
R
R
Rübsamen-Waigmann
H
H
D001483:Base Sequence; D002454:Cell Differentiation; D015658:HIV Infections / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology*; D016209:Interleukin-8 / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D016173:Macrophage Colony-Stimulating Factor / Q000378:metabolism*; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism* / Q000382:microbiology; D008969:Molecular Sequence Data; D009000:Monocytes / Q000502:physiology; D012333:RNA, Messenger / Q000187:drug effects / Q000378:metabolism*
D016209:Interleukin-8; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D016173:Macrophage Colony-Stimulating Factor; D007371:Interferon-gamma