title: Domain structure, functional activity, and polymerization of trout complement protein C9.
abstract: The 3' region of trout C9 has been resequenced and found to differ from the previously published sequence (Stanley and Herz, EMBO J. 6:1951; 1987). In contrast to other sequenced C9 molecules, but in common with the other terminal complement components, trout C9 was found to contain an additional carboxy terminal thrombospondin domain. This domain does not restrict polymerization, as has been previously suggested (Stanley and Luzio, Nature 334:475; 1988), since alternative pathway activation of trout complement by rabbit erythrocytes lead to the formation of circular membrane attack complement lesions on the erythrocyte membrane. Although the trout C9 molecule is larger than human C9, the diameters of circular trout membrane attack complexes were approximately 30% smaller than their human counterparts. No lysis of erythrocytes bearing human C5b-7 or C5b-8 complexes was detected following incubation with trout serum containing EDTA, which suggests that trout C8 and C9 are unable to bind to human C7 and C8, respectively. Finally, trout and human serum were equally effective at killing the human serum-sensitive strain Salmonella minnesota Re595.
authors.affiliation: Department of Comparative and Experimental Pathology, University of Florida, Gainesville 32610.
authors.forename: S
authors.initials: S
authors.lastname: Tomlinson
authors.forename: K K
authors.initials: KK
authors.lastname: Stanley
authors.forename: A F
authors.initials: AF
authors.lastname: Esser
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003186:Complement C9 / Q000737:chemistry*; D006461:Hemolysis; D006801:Humans; D008322:Mammals / Q000097:blood; D008969:Molecular Sequence Data; D010980:Platelet Membrane Glycoproteins; D011487:Protein Conformation; D012475:Salmonella; D019699:Thrombospondins; D014337:Trout / Q000378:metabolism*
chemical_list: D003186:Complement C9; D010980:Platelet Membrane Glycoproteins; D019699:Thrombospondins