Pleiotrophic effects of point mutations in yeast tRNA(Asp) on the base modification pattern.
The base-modification pattern has been studied in several synthetic variants of yeast tRNA(Asp) injected into Xenopus laevis oocytes. Certain point mutations in the D-stem and the variable loop of the tRNA led to considerably decreased levels of m1G37, psi 40 and Q34/manQ34 in the anticodon stem or loop and an increased rate of synthesis for m5C49 in the T-stem. The formation of m2G6 in the aminoacyl-stem was not affected in any of the tRNA-variants. Thus, mutations in one part of the tRNA-molecule can have long-range effects on the interactions between another part of the tRNA and the tRNA modifying enzymes.
Edqvist
J
J
Department of Microbiology, University of Umeå, Sweden.
Stråby
K B
KB
Grosjean
H
H
D000818:Animals; D000926:Anticodon; D001482:Base Composition; D001483:Base Sequence; D002478:Cells, Cultured; D007700:Kinetics; D008845:Microinjections; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009865:Oocytes; D017354:Point Mutation; D012323:RNA Processing, Post-Transcriptional; D012349:RNA, Transfer, Asp / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics; D014982:Xenopus laevis
D000926:Anticodon; D012349:RNA, Transfer, Asp