Provirus-anchored long-range (PAL) mapping of mammalian genomes.
To design a strategy for more efficient long-range physical mapping of large mammalian genomes, we have developed a method for directional mapping from anchor points of randomly integrated retroviral proviruses containing sequences that provide unique cleavage sites in the genome. Using this method, we have determined a physical map of approximately 1.6 Mb flanking an Mo MLV-proviral integration site in mouse NIH/3T3 cells. Our results indicate that this provirus-anchored long-range (PAL) mapping strategy, which uses only two proviral sequences as universal hybridization probes, can potentially allow rapid construction of physical maps for chromosomal regions devoid of other genetic markers.
Kurdi-Haidar
B
B
Center for Molecular Genetics, UCSD School of Medicine, La Jolla 92093-0634.
Levine
F
F
Roemer
K
K
LaPorte
P
P
Friedmann
T
T
D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D002874:Chromosome Mapping / Q000379:methods*; D015698:Genomic Library; D008322:Mammals; D051379:Mice; D008969:Molecular Sequence Data; D011533:Proviruses / Q000235:genetics* / Q000502:physiology; D051381:Rats; D015183:Restriction Mapping; D012190:Retroviridae / Q000235:genetics; D016662:Virus Integration