Organization of a 117-kb circular mitochondrial chromosome in IR36 rice.
The organization of the mitochondrial genome in the IR36 indica variety of rice was analyzed using constant-field and CHEF gel electrophoresis. The minimum complexity of the genome is estimated to be 300 kb. There was no evidence for a master circular molecule of this size. The genome exists in vivo in multiple subgenomic circular molecules or mitochondrial chromosomes. Using several rare-cutting restriction enzymes a restriction map was constructed for a 117-kb circular chromosome. Six genetic loci have been placed on this chromosome; cox1 and atp1 have been assigned precise positions while four additional genes (rrn26, cob2, atp6 and rrn18) were mapped to restriction fragments.
Narayanan
K K
KK
Department of Biological Sciences, Stanford University, CA 94305-5020.
André
C P
CP
Yang
J
J
Walbot
V
V
D015139:Blotting, Southern; D002874:Chromosome Mapping; D004272:DNA, Mitochondrial / Q000235:genetics*; D016521:Electrophoresis, Gel, Pulsed-Field; D017343:Genes, Plant; D012275:Oryza / Q000235:genetics*; D015183:Restriction Mapping
D004272:DNA, Mitochondrial