A protein histidine kinase induced in rat liver by peroxisome proliferators. In vitro activation by Ras protein and guanine nucleotides.
A novel protein kinase is induced in rat liver plasma membrane by the administration of peroxisome proliferators. A 36 kDa protein (P36) on the membrane was rapidly phosphorylated in vitro by the kinase and the phosphorylated amino acid was identified as phosphohistidine. Histidine phosphorylation of P36 was activated in vitro by recombinant Ras protein and GTP; both decreased Michaelis constant (Km) for ATP from 1.25 to 0.25 microM. The novel histidine kinase, products of which have been overlooked due to their acid lability, may participate in cellular signaling and peroxisome proliferators may perturb the pathway.
Motojima
K
K
Department of Biochemistry, School of Pharmaceutical Sciences, Toho University, Chiba, Japan.
Goto
S
S
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002994:Clofibrate / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D006150:Guanine Nucleotides / Q000494:pharmacology*; D006160:Guanosine Triphosphate / Q000494:pharmacology; D006639:Histidine / Q000378:metabolism; D000071677:Histidine Kinase; D008099:Liver / Q000201:enzymology* / Q000648:ultrastructure; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism; D008830:Microbodies / Q000201:enzymology*; D010766:Phosphorylation; D011494:Protein Kinases / Q000096:biosynthesis*; D016283:Proto-Oncogene Proteins p21(ras) / Q000494:pharmacology*; D051381:Rats; D011916:Rats, Inbred F344; D011994:Recombinant Proteins / Q000494:pharmacology
D006150:Guanine Nucleotides; D008565:Membrane Proteins; D011994:Recombinant Proteins; D006639:Histidine; D006160:Guanosine Triphosphate; D000255:Adenosine Triphosphate; D011494:Protein Kinases; D000071677:Histidine Kinase; D016283:Proto-Oncogene Proteins p21(ras); D002994:Clofibrate