title: Organophosphate sensitizes the human pancreas to acinar cell injury: an ultrastructural study.
abstract: Viable pancreas fragments from five human donors were incubated in oxygenated buffered Eagle Medium. The preparation and incubation conditions were based on the method of Scheele and Palade. In Group 1 there was 1-h preincubation with echothiophate (10(-4) M); then, acetylcholine (10(-5) M) was added. After 2 h tissues were prepared for electron microscopy. Acinar injury with vacuole formation was apparent. Many of these changes were observed in fragments incubated only with acetylcholine (10(-5) M) (Group 2) and in incubates with echothiophate only (10(-4) M) (Group 3); only minor changes were seen in controls with Eagle's Medium (Group 4). Large vacuoles were significantly more numerous in Group 1 than in Control Group 4 (p < 0.05). Zymogen granules were depleted in Groups 1, 2, and 3. This depletion was significant in Group 1 when compared with Group 4 (p < 0.02). These results extend previous in vitro results that showed increased amylase release after echothiophate treatment in human pancreas and a left shift in response to acetylcholine.
authors.affiliation: Department of Surgery, University of Minnesota School of Medicine, Minneapolis.
authors.forename: R L
authors.initials: RL
authors.lastname: Goodale
authors.forename: J C
authors.initials: JC
authors.lastname: Manivel
authors.forename: J W
authors.initials: JW
authors.lastname: Borner
authors.forename: S
authors.initials: S
authors.lastname: Liu
authors.forename: J
authors.initials: J
authors.lastname: Judge
authors.forename: C
authors.initials: C
authors.lastname: Li
authors.forename: T
authors.initials: T
authors.lastname: Tanaka
mesh_terms: D000109:Acetylcholine / Q000633:toxicity; D000208:Acute Disease; D004456:Echothiophate Iodide / Q000633:toxicity*; D006801:Humans; D066298:In Vitro Techniques; D008854:Microscopy, Electron; D010179:Pancreas / Q000648:ultrastructure; D010195:Pancreatitis / Q000139:chemically induced* / Q000473:pathology
chemical_list: D004456:Echothiophate Iodide; D000109:Acetylcholine