title: Determination of the secondary structure of epoxide hydrolase by Raman spectroscopy.
abstract: The secondary structure of microsomal epoxide hydrolase was determined by Raman spectroscopy and the effect of the membrane microenvironment studied. The ratios of the four secondary structure contents, alpha-helix: beta-strand:turn:undefined, were found to be 47:24:17:11 and 58:17:15:10 for the solubilized and the membrane-bound epoxide hydrolase, respectively. Based on the spectral analysis in the 2800-2900 cm-1 range, it was concluded that the protein studied produces the disordering effect on the lipid dimyristoylphosphatidylcholine bilayer at 16 degrees C.
authors.affiliation: Institute of Biological and Medical Chemistry, Moscow, Russia.
authors.forename: Y D
authors.initials: YD
authors.lastname: Ivanov
authors.forename: A A
authors.initials: AA
authors.lastname: Izotov
authors.forename: I G
authors.initials: IG
authors.lastname: Rukavishnikov
authors.lastname: Uvarov VYu
mesh_terms: D000818:Animals; D004134:Dimyristoylphosphatidylcholine; D004851:Epoxide Hydrolases / Q000737:chemistry* / Q000494:pharmacology; D008051:Lipid Bilayers / Q000737:chemistry; D008433:Mathematics; D008861:Microsomes / Q000201:enzymology; D015394:Molecular Structure; D017433:Protein Structure, Secondary; D051381:Rats; D013059:Spectrum Analysis, Raman / Q000379:methods
chemical_list: D008051:Lipid Bilayers; D004851:Epoxide Hydrolases; D004134:Dimyristoylphosphatidylcholine