title: Mineralization of dentinal collagen sheets complexed with alkaline phosphatase and integration with newly formed bone following subperiosteal implantation over osseous defects in rat calvaria.
abstract: We addressed the question to what extent alkaline phosphatase (ALP) can induce mineralization of a collagenous matrix implanted subperiosteally, and how the graft interacts with the underlying bone. Bovine intestinal ALP was bound to sheets of guanidine-extracted, demineralized bovine dentin by using the crosslinking agent 1-ethyl-3(3-dimethylaminopropyl)carbodiimide.HCl. The complexes (with active enzyme) and control grafts (no enzyme) were implanted over osseous defects in opposite halves of rat calvaria. After time intervals varying from 3-12 weeks, the calvaria were processed for light and electron microscopic examination and histomorphometric analysis. The ALP-containing sheets (but not their controls) rapidly accumulated mineral crystals. As the complexes mineralized, osteoblasts appeared and formed a layer of bone in direct contact with the grafted material. The results indicate that ALP induced the deposition of mineral crystals, and strongly suggest that it is this mineral component which influenced the formation of bone.
authors.affiliation: Department of Periodontology, Academic Center for Dentistry, Amsterdam, ACTA, Netherlands.
authors.forename: W
authors.initials: W
authors.lastname: Beertsen
authors.forename: T
authors.initials: T
authors.lastname: van den Bos
authors.forename: J
authors.initials: J
authors.lastname: Niehof
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism*; D000818:Animals; D015519:Bone Density / Q000502:physiology; D016025:Bone Transplantation / Q000473:pathology / Q000502:physiology*; D002113:Calcification, Physiologic / Q000502:physiology*; D003094:Collagen / Q000378:metabolism*; D003804:Dentin / Q000378:metabolism; D005260:Female; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
chemical_list: D003094:Collagen; D000469:Alkaline Phosphatase