title: Bactericidal activity of gentamicin against S. aureus. In vitro study questions value of prolonged high concentrations.
abstract: Bactericidal activity of gentamicin against 4 strains of Staphylococcus aureus was investigated in vitro using Mueller-Hinton broth (MH) and surgical wound fluid (WF) as test media. 6 concentrations of gentamicin were tested ranging from 1-1024 micrograms/mL. During the first hour of incubation, there was a marked concentration-dependent kill rate, approximately 3 x 10(3) times in WF and 10(2) times in MH. After the first hour of incubation, the kill rate was independent of the concentration in both media. Our study questions the benefit of the prolonged and high wound concentrations of gentamicin obtained after application of gentamicin-containing PMMA.
authors.affiliation: Department of Orthopedics, Frederiksberg Hospital, Denmark.
authors.forename: T S
authors.initials: TS
authors.lastname: Sørensen
authors.forename: A I
authors.initials: AI
authors.lastname: Sørensen
mesh_terms: D005839:Gentamicins / Q000008:administration & dosage / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D013045:Species Specificity; D013211:Staphylococcus aureus / Q000187:drug effects*; D013530:Surgical Wound Infection / Q000188:drug therapy / Q000382:microbiology
chemical_list: D005839:Gentamicins