A hamster-human subchromosomal hybrid cell panel for chromosome 2.
We have constructed hamster-human hybrid cell lines containing fragments of human chromosome 2 as their only source of human DNA. Microcell-mediated chromosome transfer was used to transfer human chromosome 2 from a monochromosomal mouse-human hybrid line to a radiation-sensitive hamster mutant (XR-V15B) defective in double-strand break rejoining. The human chromosome 2 carried the Ecogpt gene and hybrids were selected using this marker. The transferred human chromosome was frequently broken, and the resulting microcell hybrids contained different sized segments of the q arm of chromosome 2. Two microcell hybrids were irradiated and fused to XR-V15B to generate additional hybrids bearing reduced amounts of human DNA. All hybrids were analyzed by PCR using primers specific for 27 human genes located on chromosome 2. From these data we have localized the integrated gpt gene on the human chromosome 2 to the region q36-37 and present a gene order for chromosome 2 markers.
Jeggo
P A
PA
MRC Cell Mutation Unit, Sussex University, Falmer, Brighton, U.K.
Hafezparast
M
M
Thompson
A F
AF
Kaur
G P
GP
Sandhu
A K
AK
Athwal
R S
RS
D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping / Q000379:methods; D002889:Chromosomes, Human, Pair 2; D006224:Cricetinae; D016172:DNA Fingerprinting; D004277:DNA, Single-Stranded; D006801:Humans; D006822:Hybrid Cells / Q000528:radiation effects; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012091:Repetitive Sequences, Nucleic Acid
D004277:DNA, Single-Stranded