Determination of clozapine and desmethylclozapine in human plasma by high-performance liquid chromatography with ultraviolet detection.
A method using reversed-phase high-performance liquid chromatography for the simultaneous determination of clozapine and its desmethyl metabolite in human plasma has been established. Clozapine and N-desmethylclozapine were extracted with n-hexane-isoamyl alcohol (98.5:1.5, v/v). Protriptyline served as the internal standard. The limits of detection for clozapine and desmethylclozapine are 2 and 1 ng/ml, respectively. The sensitivity and precision of this method can be utilized for pharmacokinetic studies and therapeutic drug monitoring regimens.
Chung
M C
MC
Taipei City Psychiatric Centre, Taiwan.
Lin
S K
SK
Chang
W H
WH
Jann
M W
MW
D000328:Adult; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003024:Clozapine / Q000031:analogs & derivatives* / Q000097:blood*; D006801:Humans; D008297:Male; D012559:Schizophrenia / Q000097:blood; D013056:Spectrophotometry, Ultraviolet
C058272:norclozapine; D003024:Clozapine