Transformation of Helicobacter pylori by electroporation.
A highly efficient method of introducing DNA into an important human pathogen is reported here. Electroporation-mediated transformation of a laboratory-passaged isolate of Helicobacter pylori was successfully used to establish genetic mutants at a transformation frequency of > 10(5)/micrograms DNA. This method should be widely applicable to all isolates of H. pylori and may eliminate the variability reported when natural transformation was used on fresh clinical isolates.
Segal
E D
ED
Department of Microbiology and Immunology, Stanford University, CA 94305.
Tompkins
L S
LS
D004269:DNA, Bacterial / Q000235:genetics; D004352:Drug Resistance, Microbial / Q000235:genetics; D004560:Electricity; D016480:Helicobacter pylori / Q000235:genetics*; D009154:Mutation; D013307:Streptomycin; D014169:Transformation, Bacterial
D004269:DNA, Bacterial; D013307:Streptomycin