title: Immunosuppressive effects of Pseudomonas aeruginosa exotoxin A on human B-lymphocytes.
abstract: In this study we investigated the effects of exotoxin A on proliferation and differentiation of human B-cells in vitro. Exotoxin A at a concentration of 1 microgram/ml inhibited the proliferation of B-cells preactivated by insolubilized anti-IgM antibody or by formalinized Staphylococcus aureus particles, plus IL-2 or IL-4. B-cell blasts obtained after preactivation of tonsillar B-cells produce IgG and IgM in culture supernatants, and this Ig production is enhanced by IL-2 or IL-4. Exotoxin A inhibited the production of IgG and IgM by the B-blasts at the concentration of 1 microgram/ml.
authors.affiliation: Centre de Recherches du Service de Santé des Armées, Unité de Microbiologie, La Tronche, France.
authors.forename: D R
authors.initials: DR
authors.lastname: Vidal
authors.forename: P
authors.initials: P
authors.lastname: Garrone
authors.forename: J
authors.initials: J
authors.lastname: Banchereau
mesh_terms: D036002:ADP Ribose Transferases; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology*; D001427:Bacterial Toxins / Q000633:toxicity*; D002454:Cell Differentiation; D005098:Exotoxins / Q000633:toxicity*; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000096:biosynthesis; D007166:Immunosuppressive Agents / Q000633:toxicity*; D007376:Interleukin-2 / Q000494:pharmacology; D008213:Lymphocyte Activation; D011550:Pseudomonas aeruginosa; D037521:Virulence Factors; D000097668:Pseudomonas aeruginosa Exotoxin A
chemical_list: D036002:ADP Ribose Transferases; D001427:Bacterial Toxins; D005098:Exotoxins; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007166:Immunosuppressive Agents; D007376:Interleukin-2; D037521:Virulence Factors; D000097668:Pseudomonas aeruginosa Exotoxin A