Effect of serotonin axon injury on the somatostatinergic system in rat frontoparietal cortex.
In order to investigate the possibility that, in the rat, some cerebral cortex somatostatin (SS) receptors may be localized presynaptically on the terminals of serotonergic neurons, serotonin [5-hydroxytryptamine, (5-HT)] neurons in the central nervous system were damaged with a local intracerebral injection of the serotonergic neurotoxin, 5,7-dihydroxytryptamine(5,7-DHT). The injection of 5,7-DHT (11 micrograms free base dissolved in 10 microliters of isotonic saline containing 0.01% ascorbic acid) in rats produced an reduction by about 74% in frontoparietal cortical 5-HT content at 1 and 3 weeks after injection. These changes were associated with a significant decrease by about 30% in the total number of specific SS receptors in the frontoparietal cortex at both times studied without influencing the apparent affinity of the receptors. Together, these results suggest that a portion of the frontoparietal cortex SS receptors may be localized presynaptically on the serotonergic nerve terminals. The 5,7-DHT did not affect SS-like immunoreactivity (SSLI) levels suggesting that SS and 5-HT are not colocalized within the same neuronal elements in the rat frontoparietal cortex.
Muñoz-Acedo
G
G
Department of Biochemistry and Molecular Biology, Medical School, University of Alcalá, Madrid, Spain.
López-Sañudo
S
S
Arilla
E
E
D015116:5,7-Dihydroxytryptamine / Q000494:pharmacology; D000818:Animals; D001369:Axons / Q000187:drug effects / Q000378:metabolism*; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism*; D005625:Frontal Lobe / Q000378:metabolism; D009410:Nerve Degeneration / Q000187:drug effects / Q000502:physiology; D010296:Parietal Lobe / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D017481:Receptors, Somatostatin / Q000187:drug effects / Q000378:metabolism*; D012044:Regression Analysis; D012701:Serotonin / Q000378:metabolism*
D017481:Receptors, Somatostatin; D015116:5,7-Dihydroxytryptamine; D012701:Serotonin