Immunohistochemical detection of prostaglandin I2 synthase in various calcified tissue-forming cells in rat.
Localization of prostaglandin (PG) I2 synthase immunoreactivity was examined in demineralized sections of rat pulpal, periodontal and skeletal tissues using isn-1, a monoclonal antibody raised against the enzyme. Various calcified tissue-forming cells, i.e. odontoblasts, osteoblasts, osteocytes, cementoblasts, cementocytes and chondrocytes, were similarly immunoreactive for PGI2 synthase, suggesting that they are capable of producing PGI2. In odontoblasts and chondrocytes, the reactivity increased gradually with maturation. Weak immunoreactivity was also observed in endothelial cells and fibroblast-like cells in pulpal and periodontal tissues. However, no reactivity was seen in ameloblasts. These results suggest the possible involvement of PGI2 in the regulation of the metabolism of various calcified tissues. Monoclonal antibodies such as isn-1 may become useful markers of the maturation of calcified tissue-forming cells of mesenchymal origin.
Okiji
T
T
Department of Endodontics, Faculty of Dentistry, Tokyo Medical and Dental University, Japan.
Morita
I
I
Kawashima
N
N
Kosaka
T
T
Suda
H
H
Murota
S
S
D000818:Animals; D001842:Bone and Bones / Q000201:enzymology*; D002356:Cartilage / Q000166:cytology / Q000201:enzymology; D003577:Cytochrome P-450 Enzyme System / Q000032:analysis*; D003739:Dental Cementum / Q000166:cytology / Q000201:enzymology; D003782:Dental Pulp / Q000098:blood supply / Q000166:cytology / Q000201:enzymology; D003804:Dentin / Q000166:cytology / Q000201:enzymology; D004727:Endothelium / Q000201:enzymology; D004730:Endothelium, Vascular / Q000201:enzymology; D005347:Fibroblasts / Q000201:enzymology; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D019746:Intramolecular Oxidoreductases; D007535:Isomerases / Q000032:analysis*; D008297:Male; D009804:Odontoblasts / Q000201:enzymology; D010006:Osteoblasts / Q000201:enzymology; D010011:Osteocytes / Q000201:enzymology; D010513:Periodontal Ligament / Q000201:enzymology; D051381:Rats; D017208:Rats, Wistar; D014070:Tooth / Q000201:enzymology*
D003577:Cytochrome P-450 Enzyme System; D007535:Isomerases; D019746:Intramolecular Oxidoreductases; C022461:prostacyclin synthetase