Sensitive and specific polymerase chain reaction detection of Toxoplasma gondii for veterinary and medical diagnosis.
A polymerase chain reaction (PCR) method was developed for the detection of Toxoplasma gondii. A universal- and a T. gondii-specific primer was used to amplify a region of the small subunit ribosomal RNA gene. This approach allows for a theoretical detection limit of 0.01 zoite of T. gondii per sample assayed. Experiments showed that this PCR method could detect 0.1 pg of T. gondii DNA, which represents about one organism. Polymerase chain reaction tests using DNAs of cat, dog, swine, cattle, human, Sarcocystis cruzi, Eimeria ahsata, E. vermiformis, and Escherichia coli indicated no cross-reaction with nucleic acids of hosts, related coccidia, or bacteria. Data on the sensitivity and specificity suggest that this PCR assay could be extremely useful for the diagnosis of toxoplasmosis in human and veterinary medicine, as well as for food safety surveys.
MacPherson
J M
JM
Agriculture Canada, Health of Animals Laboratory, Saskatoon, Saskatchewan.
Gajadhar
A A
AA
D000818:Animals; D001483:Base Sequence; D002415:Cats; D002417:Cattle; D016054:DNA, Protozoan / Q000032:analysis*; D004285:Dogs; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000662:veterinary*; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D012680:Sensitivity and Specificity; D013552:Swine; D014122:Toxoplasma / Q000235:genetics / Q000302:isolation & purification*; D014124:Toxoplasmosis, Animal / Q000175:diagnosis*
D016054:DNA, Protozoan; D012337:RNA, Ribosomal, 18S