title: Sequence similarity of a hornet (D. maculata) venom allergen phospholipase A1 with mammalian lipases.
abstract: We have determined the sequence of a venom allergen phospholipase A1 from white-faced hornet (Dolichovespula maculata) by cDNA and protein sequencings. This protein of 300 amino acid residues (Dol m I) has no sequence similarity with other known phospholipases. But it has sequence similarity with mammalian lipases; about 40% identity in overlaps of 123 residues. Tests suggest that hornet phospholipase has weak lipase activity. Hornet venom has 3 major allergens, and another hornet allergen antigen 5 (Dol m V) was previously found to have sequence similarity with a mammalian testis protein and a plant leaf protein.
authors.affiliation: Rockefeller University, New York, NY 10021.
authors.forename: L
authors.initials: L
authors.lastname: Soldatova
authors.forename: L
authors.initials: L
authors.lastname: Kochoumian
authors.forename: T P
authors.initials: TP
authors.lastname: King
mesh_terms: D000485:Allergens / Q000737:chemistry*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000737:chemistry; D008049:Lipase / Q000737:chemistry* / Q000378:metabolism; D008969:Molecular Sequence Data; D010741:Phospholipases A / Q000737:chemistry* / Q000378:metabolism; D054466:Phospholipases A1; D017386:Sequence Homology, Amino Acid; D014862:Wasp Venoms / Q000737:chemistry*; D014863:Wasps / Q000737:chemistry / Q000201:enzymology
chemical_list: D000485:Allergens; D014862:Wasp Venoms; D004247:DNA; D008049:Lipase; D010741:Phospholipases A; D054466:Phospholipases A1; C080423:allergen Dol M I