A localization study of the cytochrome P-450(21)-linked monooxygenase system in adult rat brain.
Immunohistochemical studies were performed to investigate the localization of the cytochrome P-450(21)-linked monooxygenase system in rat brain using a specific antibody against bovine adrenal cytochrome P-450(21) (P-450XXIA1), which was purified electrophoretically as a single protein band and with a heme content of 18.0 nmol/mg protein from adrenocortical microsomes. The cytochrome P-450(21) was found to be mainly localized in the tractus reticulothalamicus and other ascending fibers in adult rat brains. This finding indicated that deoxycorticosterone or its derivatives could be implicated in the regulation of consciousness and the induction of an anesthetic effect.
Iwahashi
K
K
Department of Biochemistry, Kagawa Medical School, Japan.
Kawai
Y
Y
Suwaki
H
H
Hosokawa
K
K
Ichikawa
Y
Y
D000818:Animals; D001921:Brain / Q000201:enzymology*; D005260:Female; D007124:Immunoenzyme Techniques; D008297:Male; D009251:NADPH-Ferrihemoprotein Reductase / Q000378:metabolism; D010784:Photomicrography; D051381:Rats; D017208:Rats, Wistar; D013255:Steroid 21-Hydroxylase / Q000032:analysis*
D013255:Steroid 21-Hydroxylase; D009251:NADPH-Ferrihemoprotein Reductase