A monozygotic twin pregnancy after application of zona rubbing on a frozen-thawed blastocyst.
Assisted hatching techniques enhance the success rate of implantation of in-vitro fertilized human embryos. We report here the successful transfer of a frozen-thawed human blastocyst on which we applied a non-invasive zona rubbing technique (reduction of the thickness of the zona pellucida by gentle rubbing with a microneedle). The implantation in the uterus led to the delivery of healthy monozygotic twins.
Nijs
M
M
IVF Centre, Van Helmont Ziekenhuis, Vilvoorde, Belgium.
Vanderzwalmen
P
P
Segal-Bertin
G
G
Geerts
L
L
Van Roosendaal
E
E
Segal
L
L
Schoysman-Deboeck
A
A
Schoysman
R
R
D001755:Blastocyst; D015925:Cryopreservation; D004624:Embryo Transfer / Q000379:methods*; D005260:Female; D006358:Hot Temperature; D006801:Humans; D008297:Male; D008846:Micromanipulation / Q000379:methods*; D011247:Pregnancy; D011272:Pregnancy, Multiple; D014430:Twins, Monozygotic; D015044:Zona Pellucida / Q000502:physiology*