The thymus hypocholesterolemic factor (TphF): a bovine thymic superoxide dismutase active on HMG-CoA reductase.
1. This work describes the further biochemical characterization of a new calf thymus protein (TphF) and its primary structure. 2. The amino acid sequences, obtained after sequence analysis of peptides derived from the endoproteinase Lys-C digestion, were subjected to a "Protein Data Bank Search" and were found to be identical with regions of bovine superoxide-dismutase (SOD). 3. These data together with those showing the identical electrophoretic migration of SOD and TphF, their same isoelectric point and their immunoreactivity with anti-SOD antibodies, confirm the similarity of these two proteins.
Mondola
P
P
Istituto di Scienze Fisiologiche Umane II Facoltà di Medicina e Chirurgia di Napoli, Italy.
Santillo
M
M
Belfiore
A
A
Camardella
L
L
Santangelo
F
F
D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors; D007122:Immunoelectrophoresis; D007525:Isoelectric Focusing; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D013482:Superoxide Dismutase / Q000737:chemistry* / Q000494:pharmacology; D013950:Thymus Gland / Q000737:chemistry*; D013951:Thymus Hormones / Q000737:chemistry* / Q000494:pharmacology
D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors; D013951:Thymus Hormones; D013482:Superoxide Dismutase