Cytoplasmic beta-actin promoter produces germ cell and preimplantation embryonic transgene expression.
The cytoplasmic beta-actin promoter, commonly used as strong promoter in many gene regulation studies, produces a pattern of male germ cell and preimplantation, embryonic gene expression in transgenic mice. In seven of ten expressing transgenic lines, a chicken beta-actin-lacZ fusion gene was expressed in adult testes. In addition, five of the ten lines demonstrated transgene expression in the preimplantation mouse embryo. This is the first example of transgene expression at the stages of both gamete and early embryo. Overall, the site or transgene integration appeared to influence transgene expression in adult tissues.
Sands
A T
AT
Department of Cell Biology, Baylor College of Medicine, Houston, Texas 77030.
Hansen
T N
TN
Demayo
F J
FJ
Stanley
L A
LA
Xin
L
L
Schwartz
R J
RJ
D000199:Actins / Q000235:genetics*; D000818:Animals; D001755:Blastocyst / Q000378:metabolism*; D002478:Cells, Cultured; D002645:Chickens / Q000235:genetics; D005347:Fibroblasts; D005786:Gene Expression Regulation; D005813:Genes, Synthetic; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D008822:Mice, Transgenic; D009928:Organ Specificity; D011401:Promoter Regions, Genetic; D013094:Spermatozoa / Q000378:metabolism*; D013737:Testis / Q000378:metabolism; D001616:beta-Galactosidase / Q000235:genetics
D000199:Actins; D001616:beta-Galactosidase