title: Amplification of Mycoplasma iowae using polymerase chain reaction.
abstract: Based on sequence data of Mycoplasma iowae recombinant DNA probe, pMI-12, two 25-base primers were synthesized for use in the polymerase chain reaction (PCR). An M. iowae species-specific 299-base pair product was amplified by the primers. An annealing temperature of 58.5 C was critical for detecting all members of this heterogeneous species while maintaining specificity of the M. iowae PCR. The minimum amount of target DNA detected by M. iowae PCR was 1 pg, which was 1000 times more sensitive than the dot-blot assay using M. iowae recombinant DNA probes.
authors.affiliation: Department of Epidemiology and Preventive Medicine, School of Veterinary Medicine, University of California, Davis 95616.
authors.forename: S
authors.initials: S
authors.lastname: Zhao
authors.forename: R
authors.initials: R
authors.lastname: Yamamoto
mesh_terms: D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics*; D008969:Molecular Sequence Data; D009174:Mycoplasma / Q000235:genetics* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D004269:DNA, Bacterial