Calbindin D-28K and NADPH-diaphorase activity are localized in different populations of periglomerular cells in the rat olfactory bulb.
Calbindin D-28k (CaBP) immunocytochemistry and NADPH-diaphorase (ND) histochemistry have been combined in the rat olfactory bulb by successive incubations of the same sections. The outer strata showed a similar neuronal staining pattern for both markers with positive periglomerular neurons (although the CaBP-stained periglomerular cells were six-fold more abundant than the ND-active ones) and larger neurons scattered in the glomerular and external plexiform layers. Both populations of periglomerular cells were distinct but they did not show specific morphological characteristics nor a predominant distribution around ND-positive and negative glomeruli. The colocalization study demonstrates that the larger ND and CaBP-stained juxtaglomerular cells, identified according to their size, location and processes branching patterns as two types of short axon cells (superficial short-axon and Van Gehuchten Cells) were also independent populations.
Alonso
J R
JR
Dpto. Biología Celular y Patología, Universidad de Salamanca, Spain.
Arévalo
R
R
Porteros
A
A
Briñón
J G
JG
Lara
J
J
Aijón
J
J
D000818:Animals; D001369:Axons / Q000648:ultrastructure; D064026:Calbindins; D005260:Female; D007124:Immunoenzyme Techniques; D008970:Molecular Weight; D009252:NADPH Dehydrogenase / Q000032:analysis*; D009474:Neurons / Q000737:chemistry* / Q000201:enzymology / Q000648:ultrastructure; D009830:Olfactory Bulb / Q000737:chemistry* / Q000201:enzymology; D051381:Rats; D017208:Rats, Wistar; D064030:S100 Calcium Binding Protein G / Q000378:metabolism*
D064026:Calbindins; D064030:S100 Calcium Binding Protein G; D009252:NADPH Dehydrogenase