An alphoid DNA sequence conserved in all human and great ape chromosomes: evidence for ancient centromeric sequences at human chromosomal regions 2q21 and 9q13.
Using vector-CENP-B box polymerase chain reaction (PCR) we isolated and cloned from a human chromosome 21-specific plasmid library, a 1 kb DNA sequence, named p alpha H21. In in situ hybridization experiments, p alpha H21 hybridized, under high stringency conditions, to the centromeric region of all the human, chimpanzee, gorilla and orangutan chromosomes. On human chromosomes p alpha H21 also identified non-centromeric sequences at 2q21 (locus D2F33S1) and 9q13 (locus D9F33S2). The possible derivation of these sequences from ancestral centromeres is discussed. Sequence analysis confirmed the alphoid nature of the whole p alpha H21 insert.
Baldini
A
A
Department of Human Genetics, Yale University School of Medicine, New Haven, CT 06510.
Ried
T
T
Shridhar
V
V
Ogura
K
K
D'Aiuto
L
L
Rocchi
M
M
Ward
D C
DC
D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002503:Centromere; D002889:Chromosomes, Human, Pair 2; D002899:Chromosomes, Human, Pair 9; D017124:Conserved Sequence; D004276:DNA, Satellite / Q000032:analysis; D005260:Female; D015723:Gene Library; D006071:Gorilla gorilla / Q000235:genetics; D015186:Hominidae / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008969:Molecular Sequence Data; D002679:Pan troglodytes / Q000235:genetics; D016133:Polymerase Chain Reaction; D011148:Pongo pygmaeus / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid; D017422:Sequence Analysis, DNA
D004276:DNA, Satellite