Determination of patulin by reversed-phase high-performance liquid chromatography with extraction by diphasic dialysis.
A simple and economical method has been developed for the determination of patulin in apple juice. The sample is extracted with ethyl acetate in a diphasic dialysis system, and the extract is cleaned up by elution from a Sep-Pak cartridge. Patulin is detected and determined by reversed-phase high-performance liquid chromatography using a Novapak C18 column and an ultraviolet detector. The lower detection limit is 1 microgram l-1 and the recovery is 85% at the 20 micrograms l-1 level.
Prieta
J
J
Departamento Patología Animal I, Facultad de Veterinaria, Universidad Complutense, Madrid, Spain.
Moreno
M A
MA
Bayo
J
J
Díaz
S
S
Suárez
G
G
Domínguez
L
L
Canela
R
R
Sanchis
V
V
D000085:Acetates; D001628:Beverages / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003956:Dialysis; D005638:Fruit / Q000737:chemistry*; D008832:Microchemistry; D010365:Patulin / Q000032:analysis*
D000085:Acetates; C007650:ethyl acetate; D010365:Patulin