Stereoselective analysis of ketorolac in human plasma by high-performance liquid chromatography.
A high-performance liquid chromatographic (HPLC) analytical method is described for the quantification of the (R)- and (S)-enantiomers of ketorolac when present together in human plasma. The method involves derivatization with thionyl chloride/(S)-1-phenylethylamine and subsequent reversed-phase chromatography of the diastereomeric (S)-1-phenylethylamides of (R)- and (S)-ketorolac. The method is suitable for the analysis of large numbers of plasma samples and has been applied in this report to a pharmacokinetic study of ketorolac enantiomers upon intramuscular administration of racemic drug to a human subject. The limit of quantification for each enantiomer of ketorolac is 50 ng/ml (signal-to-noise ratio > 10).
Hayball
P J
PJ
Pharmacy Department, Repatriation General Hospital, Australia.
Tamblyn
J G
JG
Holden
Y
Y
Wrobel
J
J
D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D020910:Ketorolac; D008968:Molecular Conformation; D015394:Molecular Structure; D012044:Regression Analysis; D013237:Stereoisomerism; D014046:Tolmetin / Q000031:analogs & derivatives* / Q000097:blood
D000894:Anti-Inflammatory Agents, Non-Steroidal; D014046:Tolmetin; D020910:Ketorolac