An enhancer unit of L-type pyruvate kinase gene is responsible for transcriptional stimulation by dietary fructose as well as glucose in transgenic mice.
We produced three lines of transgenic mice containing the 5' flanking region of the L-type pyruvate kinase gene from nucleotides -189 to +37, which includes an enhancer unit and TATA box as functional elements, linked to the chloramphenicol acetyltransferase gene. Since transgene expression was stimulated by both dietary fructose and glucose in a tissue-dependent manner, we suggest that this unit is responsive to both stimuli.
Noguchi
T
T
Department of Nutrition and Physiological Chemistry, Osaka University Medical School, Japan.
Okabe
M
M
Wang
Z
Z
Yamada
K
K
Imai
E
E
Tanaka
T
T
D000818:Animals; D015152:Blotting, Northern; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D004032:Diet; D004742:Enhancer Elements, Genetic; D005632:Fructose / Q000008:administration & dosage / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects; D005947:Glucose / Q000494:pharmacology*; D051379:Mice; D008822:Mice, Transgenic; D011770:Pyruvate Kinase / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D012045:Regulatory Sequences, Nucleic Acid; D016385:TATA Box; D014158:Transcription, Genetic / Q000187:drug effects*
D012333:RNA, Messenger; D005632:Fructose; D015500:Chloramphenicol O-Acetyltransferase; D011770:Pyruvate Kinase; D005947:Glucose