title: Sensitive high-performance liquid chromatographic assay for praziquantel in plasma, urine and liver homogenates.
abstract: A high-performance liquid chromatographic method for the determination of praziquantel in plasma, urine and rat liver homogenates has been developed. It requires 2 ml of biological fluid, an extraction using Sep-Pak cartridges, a 0.05 M phosphate buffer solution (pH 5.0) for equilibrating and washing and ethyl acetate-diisopropyl ether for drug elution. The analysis was performed on an Ultrasphere ODS C18 column with a mobile phase of acetonitrile-water with ultraviolet detection at 217 nm. The results showed that the assay is sensitive (31.2 ng/ml), linear between 0.125 and 4.0 micrograms/ml, precise (coefficient of variation = 10%) and selective with other drugs currently administered with praziquantel.
authors.affiliation: Laboratory of Neuropharmacology, National Institute of Neurology and Neurosurgery of Mexico, Mexico City.
authors.forename: D F
authors.initials: DF
authors.lastname: González-Esquivel
authors.forename: C M
authors.initials: CM
authors.lastname: Okuno
authors.forename: M
authors.initials: M
authors.lastname: Sánchez Rodríguez
authors.forename: J
authors.initials: J
authors.lastname: Solelo Morales
authors.forename: H J
authors.initials: HJ
authors.lastname: Cook
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D008099:Liver / Q000737:chemistry*; D011223:Praziquantel / Q000032:analysis* / Q000097:blood / Q000652:urine; D051381:Rats; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet
chemical_list: D011223:Praziquantel