title: Influence of haptic materials on the adherence of staphylococci to intraocular lenses.
abstract: A recent case-control study indicated that the insertion of an intraocular lens with polypropylene (Prolene) haptic materials was a significant risk factor for postoperative endophthalmitis (odds ratio = 4.5, P < .01). In the present study, we used quantitative techniques to evaluate adherence of Staphylococcus epidermidis to two intraocular lens types--lenses with polypropylene haptic materials and all-polymethyl methacrylate optic and three-piece all-polymethyl methacrylate lenses--using a quantitative culture method, a radioisotope technique, and scanning electron microscopy. All three methods demonstrated approximately twice as many bacteria adherent to lenses with polypropylene haptic materials as to all-polymethyl methacrylate lenses. Scanning electron microscopy showed preferential bacterial adherence to the polypropylene haptic materials. These data provide a pathogenic mechanism to explain our epidemiologic findings of an increased risk of postoperative endophthalmitis associated with implantation of intraocular lenses with polypropylene haptic materials.
authors.affiliation: Department of Ophthalmology, New York Eye and Ear Infirmary, NY 10003.
authors.forename: E M
authors.initials: EM
authors.lastname: Raskin
authors.forename: M G
authors.initials: MG
authors.lastname: Speaker
authors.forename: S A
authors.initials: SA
authors.lastname: McCormick
authors.forename: D
authors.initials: D
authors.lastname: Wong
authors.forename: J A
authors.initials: JA
authors.lastname: Menikoff
authors.forename: K
authors.initials: K
authors.lastname: Pelton-Henrion
mesh_terms: D000327:Adsorption; D001422:Bacterial Adhesion; D015169:Colony Count, Microbial; D009877:Endophthalmitis / Q000453:epidemiology / Q000209:etiology / Q000382:microbiology; D004866:Equipment Contamination; D007553:Isotope Labeling; D007910:Lenses, Intraocular / Q000592:standards*; D008422:Materials Testing; D020366:Methylmethacrylate; D008768:Methylmethacrylates / Q000592:standards; D008855:Microscopy, Electron, Scanning; D011126:Polypropylenes / Q000592:standards*; D011183:Postoperative Complications / Q000453:epidemiology / Q000209:etiology / Q000382:microbiology; D012307:Risk Factors; D013010:Sonication; D013212:Staphylococcus epidermidis / Q000254:growth & development / Q000302:isolation & purification
chemical_list: D008768:Methylmethacrylates; D011126:Polypropylenes; D020366:Methylmethacrylate