title: Zinc finger-like motifs in rat ribosomal proteins S27 and S29.
abstract: The primary structures of the rat 40S ribosomal subunit proteins S27 and S29 were deduced from the sequences of nucleotides in recombinant cDNAs and confirmed by determination of amino acid sequences in the proteins. Ribosomal protein S27 has 83 amino acids and the molecular weight is 9,339. Hybridization of cDNA to digests of nuclear DNA suggests that there are 4-6 copies of the S27 gene; the mRNA for the protein is about 620 nucleotides in length. Ribosomal protein S29 has 55 amino acids and the molecular weight is 6,541. There are 14-17 copies of the S29 gene and its mRNA is about 500 nucleotides in length. Rat ribosomal protein S29 is related to several members of the archaebacterial and eubacterial S14 family of ribosomal proteins. S27 and S29 have zinc finger-like motifs as do other proteins from eukaryotic, archaebacterial, eubacterial, and mitochondrial ribosomes. Moreover, ribosomes and ribosomal subunits appear to contain zinc and iron as well.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Chicago, IL 60637.
authors.forename: Y L
authors.initials: YL
authors.lastname: Chan
authors.forename: K
authors.initials: K
authors.lastname: Suzuki
authors.forename: J
authors.initials: J
authors.lastname: Olvera
authors.forename: I G
authors.initials: IG
authors.lastname: Wool
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001483:Base Sequence; D004247:DNA; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008667:Metalloproteins; D008969:Molecular Sequence Data; D009687:Nuclear Proteins; D011485:Protein Binding; D012335:RNA, Ribosomal / Q000378:metabolism; D016601:RNA-Binding Proteins; D051381:Rats; D012269:Ribosomal Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D015032:Zinc / Q000032:analysis; D016335:Zinc Fingers
chemical_list: D000596:Amino Acids; D008667:Metalloproteins; D009687:Nuclear Proteins; D012335:RNA, Ribosomal; D016601:RNA-Binding Proteins; C083686:RPS27 protein, human; D012269:Ribosomal Proteins; C489993:Rps27 protein, rat; C079855:ribosomal protein S29; C041308:ribosomal protein S40; D004247:DNA; D015032:Zinc