Tetrahydropapaveroline in brain regions of rats after acute ethanol administration.
Tetrahydropapaveroline (THP), the condensation product of dopamine, and its aldehyde, dopaldehyde, have been detected in brain regions of rats after acute ethanol administration. THP levels were determined in eight brain regions of animals that received ethanol (3.0 g/kg) by intraperitoneal injection 100 or 120 minutes before decapitation. The levels of THP in two brain regions, i.e., the midbrain and striatum, were determined at time intervals ranging from 50 to 120 minutes after ethanol administration. THP was not found in brain regions of untreated animals. However, significant levels of THP were found in pooled midbrains (0.50 pmol/g tissue) and pooled hypothalami (0.20 pmol/g tissue) of animals that received ethanol 120 minutes before decapitation. Most brain regions had detectable levels of THP 100 minutes after the animals received ethanol and the striatum contained the highest concentration of the alkaloid. The concentration of THP in striata tissue of rats at 50, 70, 90, or 100 minutes after ethanol administration were 0.33, 0.38, 0.33, and 0.33 pmol/g tissue, respectively. These results demonstrate that THP can be detected in specific brain regions of the rat after acute ethanol administration.
Cashaw
J L
JL
Veterans Affairs Medical Center, Houston, TX 77030.
D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D003342:Corpus Striatum / Q000378:metabolism; D004298:Dopamine / Q000378:metabolism; D000431:Ethanol / Q000008:administration & dosage / Q000494:pharmacology*; D005625:Frontal Lobe / Q000378:metabolism; D007031:Hypothalamus / Q000378:metabolism; D007700:Kinetics; D008636:Mesencephalon / Q000378:metabolism; D008745:Methylation; D009833:Olfactory Pathways / Q000378:metabolism; D051381:Rats; D013765:Tetrahydropapaveroline / Q000378:metabolism*
D000431:Ethanol; D013765:Tetrahydropapaveroline; D004298:Dopamine