Expression of recombinant human glutathione reductase in eukaryotic cells after DNA-mediated gene transfer.
Glutathione reductase (EC 1.6.4.2) protects tissues from oxidant stress by catalyzing the NADPH-mediated reduction of glutathione disulfide to glutathione. We describe construction of a vector for DNA-mediated gene transfer and successful transient overexpression of human glutathione reductase cDNA in CHO cells. No expression was observed when the same vector was transiently transfected into NIH3T3 or LA4 cells or stably integrated in CHO cells. These results demonstrate the ability to constitute recombinant glutathione reductase expression in eukaryotic cells but suggest that this expression may be toxic.
Cholin
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Department of Cell Biology, Baylor College of Medicine, Houston, Texas 77030.
Tonoki
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Hansen
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Ledley
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D016475:3T3 Cells; D000818:Animals; D001483:Base Sequence; D016466:CHO Cells; D003001:Cloning, Molecular; D006224:Cricetinae; D005980:Glutathione Reductase / Q000096:biosynthesis*; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000096:biosynthesis*; D014162:Transfection
D011994:Recombinant Proteins; D005980:Glutathione Reductase