[Microwave stimulated cell marker analysis. Possibilities for more rapid immune diagnosis].
We describe a successful rapid APAAP-complex technique using innovative application of microwave irradiation (MIWI) on Ficoll separated peripheral blood mononuclear cell smears of healthy donors. The typing with several monoclonal antibodies (MoAbs) against different cell surface antigens is compared with the conventional APAAP procedure. The commercial domestic microwave oven was operated at 2.45 GHz. Fifteen second irradiation at 350 W during all incubation steps, e.g. primary antibody, bridging antibody and APAAP-complexes produced excellent color reactions with Fast Red TR, Fast Blue BB, New Fuchsin or NBT similar with the conventional immunoenzyme procedure. The routinely usage of a Silicon-Chamber-System developed by us is applicable without limitation under microwave conditions. The results till now have shown that the application of microwave-technique (MIWI) eliminated the need for much longer incubation periods without lost of sensitivity. All immunological markers could be detected in the same degree as observed with the conventional method. We could demonstrate that an immunological diagnosis is possible within 30 minutes using air dried smears in an microwave oven.
Ebener
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U
J. W. Goethe-Universität, Abteilung für Pädiatrische Hämatologie und Onkologie, Frankfurt/Main.
Wehner
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S
D015703:Antigens, CD / Q000032:analysis; D000943:Antigens, Differentiation / Q000032:analysis*; D002648:Child; D006801:Humans; D007124:Immunoenzyme Techniques / Q000295:instrumentation*; D016130:Immunophenotyping / Q000295:instrumentation; D008872:Microwaves; D009000:Monocytes / Q000276:immunology*; D012016:Reference Values
D015703:Antigens, CD; D000943:Antigens, Differentiation