Arbitrary primer polymerase chain reaction, a powerful method to identify Bacillus thuringiensis serovars and strains.
Arbitrary primer polymerase chain reaction technology has been applied to the identification of commercial strains of Bacillus thuringiensis by using total DNAs extracted from single bacterial colonies as templates. Characteristic DNA banding patterns can be readily and reproducibly obtained by agarose gel electrophoresis. This method has been used to distinguish commercial products containing B. thuringiensis serovar kurstaki (3a3b). When a single primer was used this method was capable of producing discriminating DNA fingerprints for 33 known serovars. Differentiation from the closely related species Bacillus cereus is also readily achieved. This technique should prove to be a powerful tool for identification and discrimination of individual B. thuringiensis strains.
Brousseau
R
R
Biotechnology Research Institute, National Research Council of Canada, Montreal.
Saint-Onge
A
A
Préfontaine
G
G
Masson
L
L
Cabana
J
J
D001409:Bacillus cereus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D001413:Bacillus thuringiensis / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D001483:Base Sequence; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000737:chemistry; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D017384:Sequence Deletion; D012703:Serotyping; D013045:Species Specificity
D004269:DNA, Bacterial