title: Stereoselective analysis of ketorolac in human plasma by high-performance liquid chromatography.
abstract: A high-performance liquid chromatographic (HPLC) analytical method is described for the quantification of the (R)- and (S)-enantiomers of ketorolac when present together in human plasma. The method involves derivatization with thionyl chloride/(S)-1-phenylethylamine and subsequent reversed-phase chromatography of the diastereomeric (S)-1-phenylethylamides of (R)- and (S)-ketorolac. The method is suitable for the analysis of large numbers of plasma samples and has been applied in this report to a pharmacokinetic study of ketorolac enantiomers upon intramuscular administration of racemic drug to a human subject. The limit of quantification for each enantiomer of ketorolac is 50 ng/ml (signal-to-noise ratio > 10).
authors.affiliation: Pharmacy Department, Repatriation General Hospital, Australia.
authors.forename: P J
authors.initials: PJ
authors.lastname: Hayball
authors.forename: J G
authors.initials: JG
authors.lastname: Tamblyn
authors.forename: Y
authors.initials: Y
authors.lastname: Holden
authors.forename: J
authors.initials: J
authors.lastname: Wrobel
mesh_terms: D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D020910:Ketorolac; D008968:Molecular Conformation; D015394:Molecular Structure; D012044:Regression Analysis; D013237:Stereoisomerism; D014046:Tolmetin / Q000031:analogs & derivatives* / Q000097:blood
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D014046:Tolmetin; D020910:Ketorolac