title: NUP2, a novel yeast nucleoporin, has functional overlap with other proteins of the nuclear pore complex.
abstract: We have isolated a new gene, NUP2, that encodes a constituent of the yeast-nuclear pore complex (NPC). The NUP2 protein sequence shares a central repetitive domain with NSP1 and NUP1, the two previously characterized yeast nucleoporins. Like NUP1 and NSP1, NUP2 localizes to discrete spots in the nuclear envelope, as determined by indirect immunofluorescence. Although the sequence similarity among these three nucleoporins suggests that they have a similar role in the nuclear pore complex, NUP2, in contrast to NSP1 and NUP1, is not required for growth. Some combinations of mutant alleles of NUP1, NSP1, and NUP2 display "synthetic lethal" relationships that provide evidence for functional interaction between these NPC components. This genetic evidence of overlapping function suggests that the nucleoporins act in concert, perhaps participating in the same step of the recognition or transit of macromolecules through the NPC.
authors.affiliation: Whitehead Institute for Biomedical Research, Cambridge, Massachusetts.
authors.forename: J D
authors.initials: JD
authors.lastname: Loeb
authors.forename: L I
authors.initials: LI
authors.lastname: Davis
authors.forename: G R
authors.initials: GR
authors.lastname: Fink
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002451:Cell Compartmentation; D002470:Cell Survival; D003001:Cloning, Molecular; D005656:Fungal Proteins / Q000235:genetics; D005800:Genes, Fungal; D008040:Genetic Linkage; D008562:Membrane Glycoproteins / Q000502:physiology*; D008969:Molecular Sequence Data; D005810:Multigene Family; D016297:Mutagenesis, Site-Directed; D009685:Nuclear Envelope / Q000648:ultrastructure; D028861:Nuclear Pore Complex Proteins; D009687:Nuclear Proteins / Q000502:physiology; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D018272:Porins / Q000235:genetics; D015183:Restriction Mapping; D029701:Saccharomyces cerevisiae Proteins; D017384:Sequence Deletion; D017386:Sequence Homology, Amino Acid; D013329:Structure-Activity Relationship
chemical_list: D005656:Fungal Proteins; D008562:Membrane Glycoproteins; C400753:NUP2 protein, S cerevisiae; D028861:Nuclear Pore Complex Proteins; D009687:Nuclear Proteins; D009838:Oligodeoxyribonucleotides; D018272:Porins; D029701:Saccharomyces cerevisiae Proteins; C049312:nuclear pore protein p62