title: Magnetic bead capture of expressed sequences encoded within large genomic segments.
abstract: Magnetic bead capture utilizes biotin-streptavidin magnetic bead technology to isolate cDNAs rapidly from large genomic intervals, giving several thousand-fold enrichment of the selected cDNAs. The technique can allow parallel analysis of several large genomic segments of varying complexities and can be applied to the isolation of expressed sequences from various tissue sources.
authors.affiliation: Department of Human Genetics, University of Michigan Medical Center, Ann Arbor 48109-0650.
authors.forename: D A
authors.initials: DA
authors.lastname: Tagle
authors.forename: M
authors.initials: M
authors.lastname: Swaroop
authors.forename: M
authors.initials: M
authors.lastname: Lovett
authors.forename: F S
authors.initials: FS
authors.lastname: Collins
mesh_terms: D001426:Bacterial Proteins; D001710:Biotin; D015825:Chromosomes, Fungal; D003001:Cloning, Molecular / Q000379:methods; D003360:Cosmids; D004247:DNA / Q000235:genetics / Q000302:isolation & purification*; D015342:DNA Probes; D015723:Gene Library; D005821:Genetic Techniques; D015894:Genome, Human; D006801:Humans; D007202:Indicators and Reagents; D008280:Magnetics; D012441:Saccharomyces cerevisiae / Q000235:genetics; D019809:Streptavidin
chemical_list: D001426:Bacterial Proteins; D015342:DNA Probes; D007202:Indicators and Reagents; D001710:Biotin; D004247:DNA; D019809:Streptavidin