Effect of beta-endorphin on sympathetic nerve activity to interscapular brown adipose tissue.
beta-Endorphin was injected into the third cerebroventricle to investigate its effects on sympathetic nerve activity to interscapular brown adipose tissue (IBAT) in rats. Multiunit discharges of sympathetic nerves to IBAT were recorded electrophysiologically in anesthetized rats. The intracerebroventricular injection of beta-endorphin (125, 250, and 500 pmol/rat in 10 microliters) suppressed sympathetic nerve activity in a dose-related fashion (-23.9 +/- 20.4, -38.7 +/- 7.1, and -66.7 +/- 7.6% 30 min after injection) compared with preinjection baseline. N-acetyl-beta-endorphin (250 pmol) had no effect on sympathetic nerve activity to IBAT. The intraperitoneal injection of naloxone (5.0 mg/rat) did not affect sympathetic nerve activity, but preinjection of naloxone inhibited the suppressive effect of intracerebroventricular injection of beta-endorphin (250 pmol). We conclude that the intracerebroventricular administration of beta-endorphin suppressed the sympathetic nerve activity to IBAT through opioid receptors. The results of this experiment are consistent with the hypothesis that beta-endorphin has a reciprocal effect on food intake and the sympathetic nervous system.
Egawa
M
M
Department of Medicine, University of Southern California School of Medicine, Los Angeles 90033.
Yoshimatsu
H
H
Bray
G A
GA
D002001:Adipose Tissue, Brown / Q000294:innervation*; D000818:Animals; D001921:Brain / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D005260:Female; D007274:Injections, Intraperitoneal; D007276:Injections, Intraventricular; D009270:Naloxone / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013564:Sympathetic Nervous System / Q000187:drug effects* / Q000502:physiology; D001615:beta-Endorphin / Q000031:analogs & derivatives / Q000037:antagonists & inhibitors / Q000494:pharmacology*
D009270:Naloxone; D001615:beta-Endorphin; C035101:N-acetyl-beta-endorphin