Comparison of two kits for measuring ferritin in blood.
Ferritin concentrations in blood are a good indicator of iron stores and can be measured in plasma or serum with commercial kits. We have measured plasma ferritin content in infants ranging from 3 to 15 months of age. During the first three years of a four-year study, plasma samples drawn from these infants were shipped to Abbott Laboratories in Chicago and assayed using the Ferrizyme immunoassay technique (Abbott Laboratories, Chicago, Illinois). For the last year of the study, we analyzed the remaining samples in St. John's using the radioimmunoassay GammaDab125 I Kit (Baxter Travenol Diagnostics, Inc., Cambridge, Massachusetts). Both kit manufacturers report inter- and intra-assay variability of < or = 5%. Results for samples analyzed by the second method were higher than earlier results from infants of the same age with the same intakes of iron; thus, we decided to analyze subsequent samples with both kits.
Matthew
D
D
Long
D
D
Andrews
W
W
Friel
J
J
D005293:Ferritins / Q000097:blood*; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation* / Q000379:methods; D007223:Infant; D011863:Radioimmunoassay / Q000295:instrumentation*; D015203:Reproducibility of Results
D005293:Ferritins