B cell development is perturbed in bone marrow from c-fos/v-jun doubly transgenic mice.
c-fos and c-jun gene products form a heterodimeric complex (AP-1) that regulates target gene expression by binding to a specific DNA sequence motif. In order to study a role of AP-1 (Fos/Jun) in growth and differentiation of immature B lineage cells, we have established and mated two independent transgenic mice carrying the mouse c-fos gene or the viral v-jun gene fused to the H-2K promoter. IL-7 dependent bone marrow cell culture from doubly transgenic (H2-fos/jun) mice demonstrated severe delay of early B cell development. Proliferation of pre-B cells in the fresh bone marrow from H2-fos/jun mice to IL-7 stimulation was very low. These results suggest that the deregulated production of AP-1 perturbs IL-7 mediated proliferation and differentiation of immature B cells.
Fujita
K
K
Department of Immunology, ICMR, Kobe University School of Medicine, Japan.
Miki
N
N
Mojica
M P
MP
Takao
S
S
Phuchareon
J
J
Nishikawa
S
S
Sudo
T
T
Tokuhisa
T
T
D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology*; D001853:Bone Marrow / Q000473:pathology*; D002454:Cell Differentiation; D002455:Cell Division; D005813:Genes, Synthetic; D016762:Genes, fos; D016758:Genes, jun; D006183:H-2 Antigens / Q000235:genetics; D006410:Hematopoiesis; D006412:Hematopoietic Stem Cells / Q000187:drug effects / Q000276:immunology; D015851:Interleukin-7 / Q000494:pharmacology; D051379:Mice; D008822:Mice, Transgenic / Q000235:genetics / Q000276:immunology*; D011401:Promoter Regions, Genetic; D016755:Proto-Oncogene Proteins c-jun / Q000235:genetics / Q000502:physiology*; D011993:Recombinant Fusion Proteins
D006183:H-2 Antigens; C024755:H-2K(K) antigen; D015851:Interleukin-7; D016755:Proto-Oncogene Proteins c-jun; D011993:Recombinant Fusion Proteins