Cloning and sequencing an ovine interleukin-4-encoding cDNA.
We have cloned a cDNA containing the complete coding sequence of ovine(ov) interleukin 4 (IL4) by the polymerase chain reaction using primers based on the 5' and 3' untranslated regions of the human IL4 gene. RNA was isolated from phorbol myristate acetate- and calcium ionphore A23187-stimulated mesenteric lymph node cells. The ovIL4 cDNA is 535 bp in length and contains an open reading frame of 408 nucleotides (nt) coding for a 15.1-kDa IL4 precursor of 135 amino acids (aa). Cleavage of the putative signal peptide of 22 aa yields the mature form of 13.2 kDa. Analysis of the mature aa sequence shows two potential N-linked glycosylation sites and six Cys residues. Ovine and bovine IL4 are shorter than human, mouse and rat IL4, because of a 51-nt deletion in the coding region. Comparison of the predicted aa sequence shows that ovIL4 shares 92, 57, 37 and 42% identity with the bovine, human, mouse and rat IL4s, respectively.
Seow
H F
HF
CSIRO Division of Animal Health, Animal Health Research Laboratory, Parkville, Victoria, Australia.
Rothel
J S
JS
Wood
P R
PR
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA; D006801:Humans; D015847:Interleukin-4 / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D012756:Sheep / Q000235:genetics*; D013045:Species Specificity
D015847:Interleukin-4; D004247:DNA