Growth properties of familial Alzheimer skin fibroblasts during in vitro aging.
Human diploid fibroblasts undergo replicative senescence in vitro, which is strongly correlated with biological aging in vivo. In order to examine whether features compatible with a systemic premature aging are present in familial Alzheimer's disease (FAD) patients, we investigated the growth characteristics of three skin fibroblast lines from FAD patients and from three sex/age-matched controls at different passages until senescence was reached. A kinetic study of the replicative capacity was performed at different culture times by [3H]-thymidine incorporation and crystal violet staining. Data showed no significant difference between the two groups at any studied passage. The life span of the two types of cultures was also comparable. These results suggest that in familial Alzheimer patients there are not systemic signs of accelerated aging.
Tesco
G
G
Department of Neurology and Psychiatry, University of Florence, Italy.
Vergelli
M
M
Amaducci
L
L
Sorbi
S
S
D000328:Adult; D000544:Alzheimer Disease / Q000235:genetics / Q000503:physiopathology*; D002455:Cell Division; D002460:Cell Line; D016922:Cellular Senescence / Q000502:physiology*; D005260:Female; D005347:Fibroblasts / Q000502:physiology; D006801:Humans; D007700:Kinetics; D008875:Middle Aged; D012867:Skin / Q000473:pathology; D013997:Time Factors