title: Expression of the FSH receptor in the testis.
abstract: FSH has multiple and changing roles in the regulation of spermatogenesis. The first function of FSH is to increase the number of Sertoli cells by stimulation of their mitotic activity. During the prepubertal phase of development, FSH is important for the maturation of the Sertoli cells. Hormonal stimulation of tight junction formation and specific protein secretion are essential. In the adult rat, some of the functions carried out by FSH in prepubertal animals are assumed by testosterone. However, there is evidence that even in the adult rat, FSH is important for quantitatively normal spermatogenesis. The gene for the FSH receptor is large (greater than 85 kb) and complex (10 introns) and is structurally similar to the genes for the LH and TSH receptor. The promoter region of the FSHR gene has been identified and is active in the expression of transgenes in transfected Sertoli cells. We have shown that the FSH receptor mRNA is present in the testes of the adult rat and that the levels of this mRNA are changing during the cycle of the seminiferous epithelium. The presence of relatively high levels of FSHR mRNA in stages XIV-II of the cycle and the relatively low levels in stages VII-VIII suggest that the FSH receptor is carefully regulated in adult rats and presumably has an important function in spermatogenesis. The levels of FSHR mRNA in cultured Sertoli cells are immediately reduced in the presence of FSH or phorbol esters, but the levels soon return to normal.
authors.affiliation: Department of Biochemistry and Biophysics, Washington State University, Pullman 99164.
authors.forename: L
authors.initials: L
authors.lastname: Heckert
authors.forename: M D
authors.initials: MD
authors.lastname: Griswold
mesh_terms: D000818:Animals; D005640:Follicle Stimulating Hormone / Q000502:physiology; D015870:Gene Expression; D008297:Male; D011962:Receptors, FSH / Q000235:genetics* / Q000502:physiology; D013737:Testis / Q000378:metabolism* / Q000502:physiology
chemical_list: D011962:Receptors, FSH; D005640:Follicle Stimulating Hormone