Quantitative analysis of tylosin by column liquid chromatography.
A column liquid chromatographic method suitable for the quality control of tylosin A is described. The determination can be carried out on different C8 or C18 columns, using a mobile phase containing acetonitrile, 0.2 M tetrabutylammonium hydrogensulphate, 0.2 M phosphoric acid and water. The flow-rate is 1 ml/min and detection is performed at 280 nm. The method shows good selectivity towards the major components tylosin A, B, C and D and demycinosyltylosin. Minor degradation products, mainly observed in solutions, are also separated. The compositions of several standards are compared and results for a number of commercial samples are presented.
Roets
E
E
Katholieke Universiteit Leuven, Laboratorium voor Farmaceutische Chemie, Belgium.
Beirinckx
P
P
Quintens
I
I
Hoogmartens
J
J
D002138:Calibration; D002240:Carbohydrate Sequence; D002853:Chromatography, Liquid / Q000379:methods*; D008969:Molecular Sequence Data; D015203:Reproducibility of Results; D015645:Tylosin / Q000032:analysis*
D015645:Tylosin