Polymerase chain reaction approaches to Culicoides (Diptera: Ceratopogonidae) identification.
Molecular genetic approaches such as polymerase chain amplification (PCR) of target genomic sequences are finding wide application in systematic and taxonomic studies of arthropods. PCR-based techniques that preclude the need for target DNA sequence information of the species of interest facilitate molecular taxonomic studies. Two such techniques, tDNA-PCR (DNA encoding tRNAs is the analyte) and RAPD-PCR (randomly amplified polymorphic DNA) were investigated for their ability to differentiate certain North American Culicoides spp. larvae and adults.
Raich
T J
TJ
Department of Microbiology, Colorado State University, Fort Collins 80523.
Archer
J L
JL
Robertson
M A
MA
Tabachnick
W J
WJ
Beaty
B J
BJ
D000818:Animals; D001483:Base Sequence; D002519:Ceratopogonidae / Q000145:classification* / Q000235:genetics; D004277:DNA, Single-Stranded / Q000737:chemistry; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012343:RNA, Transfer / Q000032:analysis*
D004277:DNA, Single-Stranded; D012343:RNA, Transfer