title: In vitro binding of [3H]bilirubin to neurons in rat brain sections.
abstract: Slide-mounted 12-microns cryostat sections of rat brain were incubated with [3H]bilirubin, washed, dried, and apposed to emulsion-coated coverslips for autoradiography. The binding appeared to be nonsaturable. Within the gray matter, binding was concentrated over neuronal cell bodies (particularly evident over hippocampal pyramidal and granular cells, and over cerebellar Purkinje cells), suggesting that the preferential neuronal toxicity of bilirubin may be related to neuronal binding. Regional differences in bilirubin binding were not demonstrated.
authors.forename: N C
authors.initials: NC
authors.lastname: Danbolt
authors.affiliation: Institute of Anatomy, National Hospital, University of Oslo, Norway.
authors.forename: C
authors.initials: C
authors.lastname: Danbolt
authors.forename: T W
authors.initials: TW
authors.lastname: Hansen
authors.forename: S
authors.initials: S
authors.lastname: Oyasoeter
authors.forename: J
authors.initials: J
authors.lastname: Storm-Mathisen
authors.forename: D
authors.initials: D
authors.lastname: Bratlid
mesh_terms: D000818:Animals; D001345:Autoradiography; D001663:Bilirubin / Q000378:metabolism*; D001921:Brain / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D066298:In Vitro Techniques; D008297:Male; D009474:Neurons / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D001663:Bilirubin