Production of monoclonal antibodies against Clostridium difficile cytotoxin using immunosorbent binding bioassay procedure.
In the following study, a novel screening approach was used to develop monoclonal antibodies specific for toxin B of Clostridium difficile. The approach, which consisted of an immunosorbent binding bioassay (ISBBA), is based on antigen immunocapture by monoclonal antibodies and detection of biological activity. Our results showed ISBBA, which uses unpurified antigen, to be more sensitive than the neutralization assay and ELISA for the detection of toxin B antibody.
Depitre
C
C
Unité d'Ecologie et de Physiologie du Système Digestif, Centre de Recherches de Jouy, Jouy-en-Josas, France.
L'Haridon
R M
RM
Lyerly
D M
DM
Barroso
L A
LA
Wilkins
T D
TD
Andremont
A
A
Corthier
G
G
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000096:biosynthesis*; D001201:Ascites / Q000276:immunology; D001426:Bacterial Proteins; D001427:Bacterial Toxins / Q000276:immunology*; D015152:Blotting, Northern; D016360:Clostridioides difficile / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006825:Hybridomas / Q000276:immunology; D007163:Immunosorbent Techniques; D051379:Mice; D009500:Neutralization Tests; D051381:Rats; D013154:Spleen / Q000166:cytology
D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins; D001427:Bacterial Toxins; C031815:toxB protein, Clostridium difficile