title: Effects of neonatal adrenalectomy on 5-HT and TRH-like immunoreactivity in the rat spinal cord.
abstract: This study investigated the effects of neonatal unilateral adrenalectomy on the serotonin- (5-HT) and thyrotropin-releasing hormone-like immunoreactivities (TRH-LI) in the intermediolateral cell column (IML) of the rat spinal cord where the sympathetic preganglionic neurons innervating the adrenal medulla are located. The density of the innervation was measured by a computer-assisted image analysis. Two weeks after the lesion, only one rat (1/3) showed a 30% decrease of 5-HT-LI in the ipsilateral IML, although no modifications were observed for TRH-LI. At 1 month, we observed a variable decrease of 19% to 30% for either 5-HT- or TRH-LI in the ipsilateral IML. However, one animal (1/3) showed nonsignificant modifications. At 3 months, we showed a 17% mean loss of 5-HT-LI and TRH-LI in the ipsilateral IML. However, the decreases of 5-HT-LI and TRH-LI did not always appear similar. These results appear different from those obtained after neonatal removal of the superior cervical ganglion.
authors.affiliation: INSERM U-336, Montpellier, France.
authors.forename: P
authors.initials: P
authors.lastname: Poulat
authors.forename: L
authors.initials: L
authors.lastname: Marlier
authors.forename: N
authors.initials: N
authors.lastname: Rajaofetra
authors.forename: A
authors.initials: A
authors.lastname: Privat
mesh_terms: D000315:Adrenalectomy; D000818:Animals; D000831:Animals, Newborn / Q000502:physiology*; D005260:Female; D005728:Ganglia, Sympathetic / Q000502:physiology; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012701:Serotonin / Q000276:immunology / Q000378:metabolism*; D013116:Spinal Cord / Q000276:immunology / Q000378:metabolism*; D013973:Thyrotropin-Releasing Hormone / Q000276:immunology / Q000378:metabolism*
chemical_list: D012701:Serotonin; D013973:Thyrotropin-Releasing Hormone