Sequence specificity of the core-binding factor.
The core-binding factor (CBF) binds the conserved core motif in mammalian type C retrovirus enhancers. We analyzed the phosphate contacts made by CBF on the Moloney murine leukemia virus enhancer by ethylation interference assay. The phosphate contacts span 9 bp centered around the consensus core site. To examine the sequence preferences for CBF binding, we employed the technique of selected and amplified binding sequence footprinting (T. K. Blackwell and H. Weintraub, Science 250:1104-1110, 1990). The consensus binding site for CBF defined by selected and amplified binding sequence footprinting is PyGPyG GTPy.
Melnikova
I N
IN
Department of Biochemistry, Dartmouth Medical School, Hanover, New Hampshire 03755.
Crute
B E
BE
Wang
S
S
Speck
N A
NA
D000818:Animals; D001483:Base Sequence; D002417:Cattle; D016384:Consensus Sequence; D004268:DNA-Binding Proteins / Q000378:metabolism*; D004742:Enhancer Elements, Genetic; D015967:Gene Expression Regulation, Viral; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D008979:Moloney murine leukemia virus / Q000235:genetics*; D009690:Nucleic Acid Conformation; D010710:Phosphates / Q000737:chemistry; D013329:Structure-Activity Relationship; D014157:Transcription Factors / Q000378:metabolism*
D004268:DNA-Binding Proteins; D010710:Phosphates; D014157:Transcription Factors