CD and 1H-NMR studies on the conformational properties of peptide fragments from the C-terminal domain of thermolysin.
The propensity of the peptide fragments 233-248, 245-260, 258-276, 279-298 and 299-316 from the thermolysin C-terminal domain to form non-random structures has been examined by CD and two-dimensional NMR spectroscopy. The conformational properties of these fragments have been studied in aqueous solution and in the mixed solvent trifluoroethanol/H2O (3:7 by vol.). Small but detectable populations of helical structures (up to 10-20%) in aqueous solution have been found for the fragments 233-248, 279-298 and 299-316. These populations are remarkably enhanced (50-70%) in the more hydrophobic mixed solvent, where the fragment 258-276 also forms a comparable helical population. These four fragments are helical in the native crystal structure and the spanning of the corresponding helices in the isolated peptides in solution matches very closely the ones in the native structure. In contrast, the fragment 245-260, an omega-loop in the crystal, remains unstructured in both solvents. Medium-range NOE between protons in sidechains indicate the adoption of preferred sidechain conformations accompanying helix formation. Results are in agreement with the framework model of folding, in which native elements of secondary structure are formed first and folding follows from the collapse of these structural elements.
Jimenez
M A
MA
Instituto de Estructura de la Materia, Madrid, Spain.
Bruix
M
M
Gonzalez
C
C
Blanco
F J
FJ
Nieto
J L
JL
Herranz
J
J
Rico
M
M
D000595:Amino Acid Sequence; D002942:Circular Dichroism; D003460:Crystallization; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D008969:Molecular Sequence Data; D015394:Molecular Structure; D010446:Peptide Fragments / Q000737:chemistry*; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D012996:Solutions; D013820:Thermolysin / Q000737:chemistry*; D014270:Trifluoroethanol; D014867:Water
D010446:Peptide Fragments; D012996:Solutions; D014867:Water; D014270:Trifluoroethanol; D013820:Thermolysin