Differentiation of seven Eimeria species by random amplified polymorphic DNA.
Eimeria species were differentiated by the polymerase chain reaction using random amplified polymorphic DNA. Seven arbitrary primers ranging in length from ten to 20 nucleotides were used with DNA of seven species of eimerian oocysts to generate unique DNA fingerprints. DNA fragments ranging from 200 to 2200 base pairs (bp) were synthesized in the different reactions. Species-specific DNA fragment mobility patterns were observed in most cases. In several assays, multiple DNA fragments were synthesized and, in the majority of assays conducted, the Eimeria species could be easily differentiated. Only six of the 49 assays performed failed to generate DNA fragments.
MacPherson
J M
JM
Health of Animals Laboratory, Agriculture Canada, Saskatoon, Sask.
Gajadhar
A A
AA
D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002645:Chickens; D003048:Coccidiosis / Q000175:diagnosis / Q000662:veterinary*; D016172:DNA Fingerprinting; D016054:DNA, Protozoan / Q000032:analysis* / Q000737:chemistry; D004539:Eimeria / Q000145:classification* / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D051381:Rats; D012756:Sheep; D013045:Species Specificity
D016054:DNA, Protozoan