Collagen-linked fluorescence in human atherosclerotic plaques.
Advanced glycosylation endproducts (AGE) are intraprotein crosslinks which form in the late stages of the Maillard (browning) reaction. It is unknown whether local changes in AGE-modified collagen occur within arteries. We measured AGE-modified collagen as collagen-linked fluorescence (CLF) in human arterial tissue and in various forms of atherosclerotic plaque. All tissues showed single fluorescence peak at excitation wavelength 340 nm and emission wavelength 420-440 nm. CLF in the aorta was 27.9 +/- 8.5 units/mg, in the coronary arteries 25.9 +/- 6.3 units/mg and in the tendon 47.8 +/- 11.5 units/mg. CLF in the skin correlated with CLF in the aorta (r = 0.467, P = 0.025) but not with CLF in coronary arteries (P = 0.935). In areas of aorta covered by superficial plaque, CLF was decreased compared with adjacent, atheroma-free segments (22.2 +/- 5.2 units/mg vs. 27.9 +/- 8.5 units/mg; P = 0.01). The CLF of collagenous plaques correlated with CLF of the atheroma-free regions. Individuals with low to moderate atheroma had lower (20.0 units/mg) CLF in superficial atherosclerotic plaques than patients with severe atheroma (22.5 units/mg; P = 0.0466). Our results indicate that local changes in vascular AGE-collagen concentration occur in atherosclerosis. This finding may have pathogenetic significance in atherosclerosis.
Lee
W K
WK
Department of Pathological Biochemistry, Western Infirmary, Glasgow, Scotland, UK.
Bell
J
J
Kilpatrick
E
E
Hayes
M
M
Lindop
G B
GB
Dominiczak
M H
MH
D000368:Aged; D000369:Aged, 80 and over; D001012:Aorta, Abdominal / Q000378:metabolism / Q000473:pathology; D001161:Arteriosclerosis / Q000378:metabolism* / Q000473:pathology; D003094:Collagen / Q000378:metabolism*; D003331:Coronary Vessels / Q000378:metabolism / Q000473:pathology; D005260:Female; D019298:Glucose-6-Phosphate; D005958:Glucosephosphates; D006031:Glycosylation; D006801:Humans; D066298:In Vitro Techniques; D015416:Maillard Reaction; D008297:Male; D008875:Middle Aged; D012867:Skin / Q000378:metabolism / Q000473:pathology; D013050:Spectrometry, Fluorescence
D005958:Glucosephosphates; D019298:Glucose-6-Phosphate; D003094:Collagen