A subset of Pr65gag is nucleus associated in murine leukemia virus-infected cells.
Nuclei of cells infected with Moloney murine leukemia virus (MoMuLV) were examined for the presence of gag proteins. This analysis was performed in conjunction with other studies suggesting a possible role for gag proteins in regulating nuclear events relating to processing and/or transport of viral genomic RNA. We detected Pr65gag and a p30-related protein in a nuclear fraction of infected cells. We also found evidence that a highly conserved amino acid sequence, which is shared by p30 and U1 small nuclear ribonucleoprotein 70-kDa protein, is a component of the nuclear targeting sequence for Pr65gag. Immunoelectron microscopy studies with a monoclonal anti-p12 antibody established that approximately 18% of gag-containing proteins of MoMuLV are located in the nucleus. Such gag-containing proteins from a mutant MoMuLV that lacks N-terminal myristic acid had greater affinity for the nucleus, suggesting that fatty acid acylation of Pr65gag plays a role in overcoming the proposed nuclear transport signal. The possible roles that nuclear gag proteins may play in retroviral replication are discussed.
Nash
M A
MA
Department of Molecular Pathology, University of Texas M. D. Anderson Cancer Center, Houston 77030.
Meyer
M K
MK
Decker
G L
GL
Arlinghaus
R B
RB
D016475:3T3 Cells / Q000648:ultrastructure; D000595:Amino Acid Sequence; D000818:Animals; D000970:Antineoplastic Agents; D001692:Biological Transport; D002451:Cell Compartmentation; D002458:Cell Fractionation; D002467:Cell Nucleus / Q000737:chemistry / Q000378:metabolism*; D003593:Cytoplasm / Q000737:chemistry / Q000378:metabolism; D004527:Egg Proteins / Q000235:genetics; D015683:Gene Products, gag / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism* / Q000648:ultrastructure; D051379:Mice; D016253:Microscopy, Immunoelectron; D008969:Molecular Sequence Data; D008979:Moloney murine leukemia virus / Q000378:metabolism*; D021382:Protein Sorting Signals; D012260:Ribonucleases / Q000235:genetics; D017412:Ribonucleoprotein, U1 Small Nuclear / Q000235:genetics
D000970:Antineoplastic Agents; D004527:Egg Proteins; D015683:Gene Products, gag; D021382:Protein Sorting Signals; D017412:Ribonucleoprotein, U1 Small Nuclear; D012260:Ribonucleases; C058703:ranpirnase