An evaluation of microwave heating for the rapid hydrolysis of peptide samples for chiral amino acid analysis.
The hydrolysis of peptide samples in 6 N deuterium chloride by microwave heating has been investigated. The influence of the power and time of heating on the recovery of the amino acids and on their racemization was studied. A high recovery of sensitive amino acids and minimal racemization, as determined by the amount of deuterium incorporation, is obtained by using an intermediate irradiation power and 30 min reaction time.
Péter
A
A
A. József University, Szeged, Hungary.
Laus
G
G
Tourwé
D
D
Gerlo
E
E
Van Binst
G
G
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry; D006358:Hot Temperature; D006868:Hydrolysis; D008872:Microwaves; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry*; D013237:Stereoisomerism
D000596:Amino Acids; D010455:Peptides