Potentiation of interferon-alpha in vitro antiviral activity by interferon-gamma is not abrogated by antibody to interferon-gamma.
The addition of minute amounts of recombinant interferon-gamma (IFN-gamma) to recombinant IFN-alpha 2 significantly enhances its antiviral activity. Although this potentiating action of IFN-gamma is destroyed by treatment with acid, it is not significantly affected by treatment with antibodies to IFN-gamma, suggesting that IFN-gamma molecules can interact with cells to potentiate IFN-alpha activity, even in the presence of neutralizing antibodies. Implications of this phenomenon are multifaceted, including the possibility that other IFN activities can be altered synergistically by combinations of different IFN types, even in the presence of specific antibodies, which have been described to occur in vivo in several pathological conditions.
Capobianchi
M R
MR
Istituto di Virologia, Università La Sapienza, Rome, Italy.
Mattana
P
P
Dianzani
F
F
D000906:Antibodies / Q000494:pharmacology; D002460:Cell Line; D004357:Drug Synergism; D000077190:Interferon alpha-2; D016898:Interferon-alpha / Q000494:pharmacology*; D007371:Interferon-gamma / Q000276:immunology / Q000494:pharmacology*; D011994:Recombinant Proteins; D012845:Sindbis Virus / Q000187:drug effects
D000906:Antibodies; D000077190:Interferon alpha-2; D016898:Interferon-alpha; D011994:Recombinant Proteins; D007371:Interferon-gamma