title: Primary structure of the major isomorph of the crustacean hyperglycemic hormone (CHH-I) from the sinus gland of the Mexican crayfish Procambarus bouvieri (Ortmann): interspecies comparison.
abstract: The amino acid sequence of this neuropeptide was elucidated by means of a combined approach of enzymatic digestions, manual and automatic Edman degradations, and mass spectrometry. It is a 72 residue peptide (molecular mass 8388 Da), with six cysteines forming three disulfide bridges connecting residues 7-43, 23-39, and 26-52, with blocked N- and C-termini, and lacking the amino acids histidine, methionine, and tryptophan. The CHH-I of Procambarus bouvieri is compared with the other known CHHs from Orconectes limosus (98.6% identity), Homarus americanus isomorph A (83.3% identity), Homarus americanus isomorph B (79.2% identity), and Carcinus maenas (61.1% identity).
authors.affiliation: Department of Biochemistry, Instituto Nacional de la Nutrición S. Zubirán, Tlalpan, Mexico, D.F.
authors.forename: A
authors.initials: A
authors.lastname: Huberman
authors.forename: M B
authors.initials: MB
authors.lastname: Aguilar
authors.forename: K
authors.initials: K
authors.lastname: Brew
authors.forename: J
authors.initials: J
authors.lastname: Shabanowitz
authors.forename: D F
authors.initials: DF
authors.lastname: Hunt
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D060829:Arthropod Proteins; D003400:Astacoidea / Q000737:chemistry*; D003386:Brachyura; D002851:Chromatography, High Pressure Liquid; D007447:Invertebrate Hormones / Q000737:chemistry* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D008121:Nephropidae; D009419:Nerve Tissue Proteins / Q000737:chemistry* / Q000302:isolation & purification; D009490:Neurosecretory Systems / Q000737:chemistry; D010449:Peptide Mapping; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
chemical_list: D060829:Arthropod Proteins; D007447:Invertebrate Hormones; D009419:Nerve Tissue Proteins; C015947:hyperglycemic hormone, crustacean