title: In vitro selection of optimal DNA substrates for T4 RNA ligase.
abstract: We have used in vitro selection techniques to characterize DNA sequences that are ligated efficiently by T4 RNA ligase. We find that the ensemble of selected sequences ligated about 10 times as efficiently as the random mixture of sequences used as the input for selection. Surprisingly, the majority of the selected sequences approximated a well-defined consensus sequence.
authors.affiliation: Salk Institute for Biological Studies, San Diego, CA 92186-5800.
authors.forename: K
authors.initials: K
authors.lastname: Harada
authors.forename: L E
authors.initials: LE
authors.lastname: Orgel
mesh_terms: D017122:Bacteriophage T4 / Q000201:enzymology*; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000737:chemistry / Q000378:metabolism*; D010761:Phosphorus Radioisotopes; D016133:Polymerase Chain Reaction / Q000379:methods; D011130:RNA Ligase (ATP) / Q000378:metabolism*; D013379:Substrate Specificity; D013698:Templates, Genetic
chemical_list: D009838:Oligodeoxyribonucleotides; D010761:Phosphorus Radioisotopes; D004247:DNA; D011130:RNA Ligase (ATP)