title: Complete development of Cryptosporidium parvum in a human endometrial carcinoma cell line.
abstract: Sporozoites of Cryptosporidium parvum, excysted from oocysts isolated from calves, were applied to monolayers of the human endometrial carcinoma cell line RL95-2. Cells were grown as monolayers in 24-well plates at concentrations ranging from 5 x 10(4) to 1 x 10(5) RL95-2 cells per well. At 1 or 7 days postculturing, C. parvum sporozoites (ranging from 1 x 10(5) to 2 x 10(5) were added to the monolayers of RL95-2 cells. The cells were fixed and stained to estimate the extent of parasite colonization. Light microscopy and electron microscopy confirmed the development and replication of C. parvum within the RL95-2 cells. A standardized and reproducible in vitro culture system for C. parvum is necessary to evaluate therapies against cryptosporidiosis.
authors.affiliation: Department of Animal, Dairy, and Veterinary Sciences, Utah State University, Logan 84322-5600.
authors.forename: K R
authors.initials: KR
authors.lastname: Rasmussen
authors.forename: N C
authors.initials: NC
authors.lastname: Larsen
authors.forename: M C
authors.initials: MC
authors.lastname: Healey
mesh_terms: D000818:Animals; D002277:Carcinoma; D016785:Cryptosporidium parvum / Q000254:growth & development*; D016889:Endometrial Neoplasms; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008855:Microscopy, Electron, Scanning; D014407:Tumor Cells, Cultured