title: The effect of copper ion on arachidonic acid metabolism in the porcine corneal epithelium.
abstract: The effect of copper ion on arachidonic acid metabolism in the corneal epithelium was studied. Our results showed that when 3H-arachidonic acid (1 microCi/microgram) was incubated with homogenate of corneal epithelium at 37 degrees C for 45 min., three metabolites were formed. Metabolite A, the major metabolite, was identified as 12-HETE by HPLC and GC. An analysis with chiral phase HPLC revealed that metabolite A was a (R)-enantiomer. The production of 12(R)-HETE was stimulated by low concentrations of CuCl2 (< 0.1 mM) and inhibited by higher concentrations of CuCl2. Study on the biological activity of 12(R)-HETE showed that it could stimulate migration of endothelial cells in a concentration as low as 10 ng/ml. These results suggest that one of the effects of copper ion on the corneal neovascularization may be to regulate the level of 12(R)-HETE.
authors.affiliation: Department of Biochemistry, National Cheng Kung University Medical College Tainan, Taiwan, Republic of China.
authors.forename: M T
authors.initials: MT
authors.lastname: Lin
authors.forename: Y H
authors.initials: YH
authors.lastname: Wang
authors.forename: Y L
authors.initials: YL
authors.lastname: Chen
authors.forename: W C
authors.initials: WC
authors.lastname: Chang
mesh_terms: D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism*; D002465:Cell Movement / Q000187:drug effects; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D003300:Copper / Q000494:pharmacology*; D003315:Cornea / Q000166:cytology / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000166:cytology; D004848:Epithelium / Q000378:metabolism; D006893:Hydroxyeicosatetraenoic Acids / Q000378:metabolism; D013552:Swine
chemical_list: D006893:Hydroxyeicosatetraenoic Acids; D016718:Arachidonic Acid; D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; D003300:Copper