title: The 17 beta-oestradiol dehydrogenase of pig endometrial cells is localized in specialized vesicles.
abstract: Two monoclonal antibodies against the 17 beta-oestradiol dehydrogenase of pig endometrial cells have been used in localization studies with immunogold electron microscopy. The antibodies attach both to a fraction of dehydrogenase-rich cytoplasmic vesicles isolated from homogenates and to vesicles of similar appearance in cells. The vesicles are filled with electron-dense material. Their tagging intensity indicates a high degree of specialization. Endometrial cells from mature animals contain a host of dehydrogenase vesicles, and cells from prepubertal animals only a few. Functional aspects of the novel organelle are discussed.
authors.affiliation: Max-Planck-Institut für experimentelle Endokrinologie, Hannover, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Adamski
authors.forename: B
authors.initials: B
authors.lastname: Husen
authors.forename: F
authors.initials: F
authors.lastname: Marks
authors.forename: P W
authors.initials: PW
authors.lastname: Jungblut
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D002458:Cell Fractionation; D003102:Colloids; D004717:Endometrium / Q000201:enzymology / Q000648:ultrastructure*; D004960:Estradiol Dehydrogenases / Q000032:analysis* / Q000378:metabolism; D005260:Female; D006046:Gold; D007140:Immunoglobulin Fab Fragments; D016253:Microscopy, Immunoelectron; D015388:Organelles / Q000201:enzymology*; D013552:Swine
chemical_list: D000911:Antibodies, Monoclonal; D003102:Colloids; D007140:Immunoglobulin Fab Fragments; D006046:Gold; D004960:Estradiol Dehydrogenases