Evidence for alpha and Mu class glutathione S-transferases in a number of fungal species.
1. The glutathione S-transferases of the following fungal species have been affinity purified on glutathione-Sepharose; Yarrowia lipolytica (NCYC825), Sporotrichum thermophile (ATCC16479), Talaromyces emersonii (ATCC26914) and Issatchenkia orientalis (CBS5147 and an isolate provided by Prof. Kumagai, Kyoto University). 2. SDS-PAGE analysis of affinity purified fungal extracts, followed by Western blots with polyclonal antisera to rat GSTs, was performed and indicated the presence of Alpha and Mu class glutathione S-transferases in the fungi immunologically and electrophoretically similar to the rat enzymes. 3. Each extract gave a characteristic affinity-gradient elution pattern on glutathione-agarose.
Sheehan
D
D
Department of Biochemistry, University College Cork, Ireland.
Casey
J P
JP
D000818:Animals; D015153:Blotting, Western; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D005658:Fungi / Q000201:enzymology*; D005982:Glutathione Transferase / Q000302:isolation & purification*; D007527:Isoenzymes / Q000302:isolation & purification*; D008970:Molecular Weight; D051381:Rats; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D013175:Sporothrix / Q000201:enzymology
D007527:Isoenzymes; D005982:Glutathione Transferase