Intracellular levels and extracellular release of lysosomal enzymes from peripheral blood monocytes in pulmonary tuberculosis patients.
The intracellular activity and extracellular release (basal and latex-stimulated) of B-glucuronidase (BG) and N-acetylglucosaminidase (NAG), measured fluorimetrically, were observed to be significantly (P < 0.05) higher in blood monocytes (BM) of untreated pulmonary tuberculosis (TB) patients compared to those of age- and sex-matched controls and Mantoux-positive subjects without any evidence of active disease. After completion of antituberculous therapy, BG and NAG activities declined appreciably (P < 0.05) and their levels became comparable to those in control subjects. The present results suggest the potentiation of the oxygen-independent defense mechanism of BM in pulmonary TB.
Jaswal
S
S
Department of Internal Medicine, Experimental Medicine and Biochemistry, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
Dhand
R
R
Sethi
A K
AK
Kohli
K K
KK
Ganguly
N K
NK
D000118:Acetylglucosaminidase / Q000097:blood*; D000293:Adolescent; D004359:Drug Therapy, Combination; D004977:Ethambutol / Q000627:therapeutic use; D005260:Female; D005966:Glucuronidase / Q000097:blood*; D006801:Humans; D007538:Isoniazid / Q000627:therapeutic use; D007840:Latex; D008247:Lysosomes / Q000201:enzymology*; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000201:enzymology*; D012016:Reference Values; D012293:Rifampin / Q000627:therapeutic use; D014397:Tuberculosis, Pulmonary / Q000097:blood* / Q000188:drug therapy / Q000201:enzymology
D007840:Latex; D004977:Ethambutol; D005966:Glucuronidase; D000118:Acetylglucosaminidase; D007538:Isoniazid; D012293:Rifampin