title: Retinoic acid decreases attachment of JAR choriocarcinoma spheroids to a human endometrial cell monolayer in vitro.
abstract: The ability of retinoic acid (RA) to modulate attachment of JAR choriocarcinoma multicellular spheroids to monolayers of a human uterine epithelial cell line (RL95-2) was examined using a centrifugal force-based adhesion assay. Exposure of choriocarcinoma spheroids to RA (10(-7) to 10(-5) M) over a 3-day culture period resulted in a dose-dependent decrease of attachment. Significant decreases in attachment were detected after 30 min (75 per cent versus 25 per cent) and 1 h (92 per cent versus 26 per cent) of confrontation-culture between choriocarcinoma and uterine cells for control versus 10(-5) M RA; by 5 h 100 per cent spheroid attachment was detected in all treatment groups. RA had no effect on cell proliferation in JAR spheroids, but 10(-5) M RA treatment induced a fivefold increase in secretion of human chorionic gonadotrophin (hCG), a known marker of conversion of cytotrophoblast to syncytiotrophoblast-like cells. These findings demonstrate that RA modulates cellular attachment and differentiation in choriocarcinoma spheroids in vitro.
authors.affiliation: Institut für Anatomie, Universitätsklinikum, Essen, Federal Republic of Germany.
authors.forename: N J
authors.initials: NJ
authors.lastname: John
authors.forename: M
authors.initials: M
authors.lastname: Linke
authors.forename: H W
authors.initials: HW
authors.lastname: Denker
mesh_terms: D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002822:Choriocarcinoma / Q000378:metabolism / Q000473:pathology*; D006063:Chorionic Gonadotropin / Q000378:metabolism; D004717:Endometrium / Q000166:cytology / Q000187:drug effects*; D005260:Female; D006801:Humans; D014212:Tretinoin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
chemical_list: D006063:Chorionic Gonadotropin; D014212:Tretinoin