Analysis and bioequivalency study on two tablet formulations of co-trimoxazole.
This paper describes an analytical procedure for simultaneous quantification of trimethoprim (TMP) and sulphamethoxazole (SMZ) in serum. Serum samples were rendered alkaline with glycine-sodium hydroxide buffer (pH 9.1) and extracted with ethyl acetate. The reconstituted sample was analysed using reversed-phase C-18 column high-performance chromatography. The mobile phase consisted of 75% triethyl ammonium acetate buffer, 20% methanol and 5% acetonitrile. The analytes were monitored at 289 nm and tetroxoprim was used as an internal standard. The maximum values for intra-day coefficients of variation (CV) for TMP-and SMZ were 5.7 and 2.1%, respectively. Interday CV values were 6.4 and 2.2%, respectively. The method was used to compare the bioavailability of two tablet formulations in terms of their pharmacokinetic parameters following oral administration of the tablets to 18 volunteers.
Sheikh Salem
M
M
Faculty of Pharmacy, Jordan University of Science and Technology, Irbid.
Alkaysi
H N
HN
Gharaibeh
A M
AM
Amari
F F
FF
Sallam
E
E
Shubair
M S
MS
D001682:Biological Availability; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D008297:Male; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013607:Tablets; D013810:Therapeutic Equivalency; D015662:Trimethoprim, Sulfamethoxazole Drug Combination / Q000097:blood* / Q000493:pharmacokinetics*
D013607:Tablets; D015662:Trimethoprim, Sulfamethoxazole Drug Combination