Expression of plasminogen activator and plasminogen activator inhibitor by rat mesothelioma induced by asbestos.
We have investigated the expression of plasminogen activators (PAs) and PA inhibitors (PAIs) by an asbestos-induced mesothelioma. Using zymographic, immunological and biochemical techniques it was demonstrated that cell lines derived from the tumor express high levels of PAI and low levels of a UK-like PA. Adherent and partially non-adherent variants of the mesothelioma expressed indistinguishable amounts of PAI and UK. Based on partial biochemical characterization, the PAI secreted by the mesothelioma cells was a set of PAIs which consisted of PAI-1 in addition to other species. These observations indicate that the difference in growth phenotype of the adherent and partially non-adherent lines was not due to detectable differences in PA and PAI expression.
Lenz
S P
SP
Joint Injury and Arthritis Research Group, University of Calgary HSC, Alberta, Canada.
Green
F H
FH
Murphy
P G
PG
Lee
M
M
Hart
D A
DA
D000818:Animals; D001194:Asbestos; D002448:Cell Adhesion; D002455:Cell Division; D002845:Chromatography; D017077:Culture Media, Conditioned; D007525:Isoelectric Focusing; D008654:Mesothelioma / Q000209:etiology / Q000378:metabolism*; D010960:Plasminogen Activators / Q000378:metabolism*; D015849:Plasminogen Inactivators / Q000378:metabolism*; D051381:Rats; D011916:Rats, Inbred F344; D014407:Tumor Cells, Cultured
D017077:Culture Media, Conditioned; D015849:Plasminogen Inactivators; D001194:Asbestos; D010960:Plasminogen Activators