Electron microscopic localization and expression on cultured cells of a novel surface-associated molecule in the rat central nervous system.
Subcellular localization and in vitro expression of a newly raised monoclonal antibody 374 antigen, which outlines a subset of neurons in various regions of the rat CNS, were studied. Electron microscopy revealed that the immunoreactivity is associated with the outer surfaces of neuronal subsets, the surfaces of glial cell processes facing such neurons and less frequently, with the Golgi apparatus and inner cell surfaces. The monoclonal antibody also stained part of the cytoplasm of a population of dendrites. In order to test whether the antigen originates from neurons, we examined the expression of the antigen in cultured cortical cells. Double immunofluorescence staining with neuron-specific enolase and glial fibrillary acidic protein revealed that the antigen is expressed on a subset of neuronal cells and to a lesser extent on glial cells. The antigen is considered to be expressed on the outer surface because the cells were stained by the monoclonal antibody irrespective of whether they were alive or fixed. The monoclonal antibody 374 antigen may be a novel neuronal surface molecule which plays a role in neuron-neuron or glial-neuron interactions in the CNS.
Murakami
F
F
Department of Biophysical Engineering, Faculty of Engineering Science, Osaka University, Japan.
Tada
Y
Y
Katsumaru
H
H
Kudo
J
J
Fujita
S C
SC
D000818:Animals; D000831:Animals, Newborn; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000032:analysis*; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology* / Q000648:ultrastructure; D005260:Female; D005455:Fluorescent Antibody Technique; D015151:Immunoblotting; D007150:Immunohistochemistry; D008297:Male; D008565:Membrane Proteins / Q000032:analysis*; D016253:Microscopy, Immunoelectron; D009474:Neurons / Q000166:cytology / Q000648:ultrastructure*; D051381:Rats; D017208:Rats, Wistar
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D008565:Membrane Proteins