title: Effects of the antioxidant butylated hydroxyanisole on cytosolic free calcium concentration.
abstract: The effect of the antioxidant butylated hydroxyanisole on free intracellular calcium was investigated in rat cardiomyocytes, rat pituitary cells, human umbilical vein endothelial cells, granulocytes and BHK-cells. The effects of incubation time and butylated hydroxyanisole (BHA) concentrations were examined with respect to the extracellular calcium concentration. Butylated hydroxyanisole increased the free cytosolic calcium concentration in a dose- and time-dependent manner irrespective of the presence of extracellular calcium. Differences between all cell types were observed in the sequel of the re-uptake of the calcium ions released by BHA. Dependence on the concentration of extracellular calcium on the extent of sequestration was seen in rat pituitary cells and cardiomyocytes only.
authors.affiliation: 2nd Department of Surgery, University of Vienna, Austria.
authors.forename: M
authors.initials: M
authors.lastname: David
authors.forename: G
authors.initials: G
authors.lastname: Horvath
authors.forename: I
authors.initials: I
authors.lastname: Schimke
authors.forename: M M
authors.initials: MM
authors.lastname: Mueller
authors.forename: I
authors.initials: I
authors.lastname: Nagy
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002083:Butylated Hydroxyanisole / Q000494:pharmacology*; D002118:Calcium / Q000032:analysis*; D006224:Cricetinae; D003600:Cytosol / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D004730:Endothelium, Vascular / Q000737:chemistry / Q000187:drug effects; D006098:Granulocytes / Q000737:chemistry / Q000187:drug effects; D006801:Humans; D007668:Kidney / Q000737:chemistry / Q000166:cytology / Q000187:drug effects; D009206:Myocardium / Q000737:chemistry / Q000166:cytology; D010902:Pituitary Gland / Q000737:chemistry / Q000166:cytology / Q000187:drug effects; D051381:Rats; D014471:Umbilical Veins / Q000166:cytology
chemical_list: D000975:Antioxidants; D002083:Butylated Hydroxyanisole; D002118:Calcium