Detection of minimal residual disease by polymerase chain reaction in patients with different hematologic diseases treated by bone marrow transplantation.
Thirteen male patients affected by different hematologic diseases who underwent bone marrow transplantation (BMT) with female donors were investigated by cytogenetic analysis and polymerase chain reaction (PCR) amplification of a DNA sequence specific for the Y chromosome. In six of these patients, PCR showed the presence of the Y chromosome-related sequence; in only three of these did cytogenetic analysis confirm the presence of mixed chimerism. In the remaining three patients, the results of the PCR were confirmed by in situ hybridization on cell nuclei with a probe for the alpha-satellite of the Y chromosome. We compare results obtained with the two methods and discuss the meaning of the minimal residual disease detected by PCR in patients submitted to BMT.
Stuppia
L
L
Istituto di Citomorfologia Umana Normale e Patologica C. N. R. Chieti, Italy.
Calabrese
G
G
Guanciali Franchi
P
P
Di Bartolomeo
P
P
Antonucci
A
A
Peila
R
R
Torlontano
G
G
Palka
G
G
D000293:Adolescent; D000328:Adult; D001483:Base Sequence; D016026:Bone Marrow Transplantation; D002648:Child; D002678:Chimera / Q000235:genetics*; D005260:Female; D006402:Hematologic Diseases / Q000235:genetics* / Q000601:surgery; D006801:Humans; D017403:In Situ Hybridization; D007621:Karyotyping; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA / Q000379:methods; D014960:X Chromosome; D014998:Y Chromosome