title: Molecular cloning, expression, and DNA sequence analysis of the gene that encodes the 16-kilodalton outer membrane lipoprotein of Serpulina hyodysenteriae.
abstract: The gene (smpA) that encodes the 16-kDa outer membrane lipoprotein of Serpulina hyodysenteriae was cloned in Escherichia coli, and its primary structure was determined by nucleotide sequencing. The putative open reading frame encodes a prolipoprotein of 16.8 kDa which in its fully acylated and cleaved form is 15.1 kDa. Analysis of the N-terminal amino acid sequence derived from the DNA sequence revealed the presence of a signal sequence and a putative acylation and signal peptidase II cleavage site (Phe-Ala-Val-Ser-Cys). In E. coli, processing of the prolipoprotein was less efficient than that observed in S. hyodysenteriae, and globomycin, an inhibitor of signal peptidase II, inhibited cleavage of the lipoprotein expressed in E. coli but did not inhibit cleavage in S. hyodysenteriae.
authors.affiliation: Agriculture and Food Research Council Institute for Animal Health, Compton, Newbury, Berks, United Kingdom.
authors.forename: W
authors.initials: W
authors.lastname: Thomas
authors.forename: R
authors.initials: R
authors.lastname: Sellwood
mesh_terms: D000595:Amino Acid Sequence; D000942:Antigens, Bacterial / Q000235:genetics; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics* / Q000276:immunology; D001483:Base Sequence; D015153:Blotting, Western; D017400:Brachyspira / Q000235:genetics*; D015870:Gene Expression; D005798:Genes, Bacterial; D008074:Lipoproteins / Q000235:genetics / Q000276:immunology; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000737:chemistry; D015183:Restriction Mapping
chemical_list: D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D008074:Lipoproteins; D009841:Oligonucleotides