In vitro vomitoxin exposure alters IgA and IgM secretion by CH12LX B cells. Relationship to proliferation and macromolecular synthesis.
The CH12LX cell line was used as a clonal model to assess the direct effects of vomitoxin on IgM and IgA secretion in B cells. When vomitoxin was included in LPS-driven CH12LX B cell cultures, it had multiple effects on Ig secretion. Whereas vomitoxin doses of 115 and 120 ng/ml caused 50% inhibition (ID50) of IgA and IgM production, respectively, toxin concentrations in the 5 to 50 ng/ml range slightly stimulated IgA production. However, low vomitoxin doses did not induce switching of membrane IgM+ CH12LX B cells to membrane IgA+. Total cell number was unaffected at vomitoxin concentrations up to 100 ng/ml but dropped markedly at 200 ng/ml (ID50 = 170 ng/ml). Using the MTT reduction assay as another measure of viability and cell function, vomitoxin was also inhibitory (ID50 = 130 ng/ml). Both thymidine incorporation and leucine incorporation were also inhibited by the toxin with estimated ID50s being 120 and 110 ng/ml, respectively. The results indicate that although at high doses, vomitoxin inhibits proliferation, Ig secretion and DNA/protein synthesis in the clonal B cell model, the toxin marginally stimulated IgA secretion at lower doses.
Minervini
F
F
Department of Food Science & Human Nutrition, Michigan State University, East Lansing.
Dong
W
W
Pestka
J
J
D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D002455:Cell Division / Q000276:immunology; D007071:Immunoglobulin A, Secretory / Q000187:drug effects*; D007075:Immunoglobulin M / Q000187:drug effects* / Q000378:metabolism; D046911:Macromolecular Substances; D051379:Mice; D014255:Trichothecenes / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D007071:Immunoglobulin A, Secretory; D007075:Immunoglobulin M; D046911:Macromolecular Substances; D014255:Trichothecenes; C007262:deoxynivalenol