The 17 beta-oestradiol dehydrogenase of pig endometrial cells is localized in specialized vesicles.
Two monoclonal antibodies against the 17 beta-oestradiol dehydrogenase of pig endometrial cells have been used in localization studies with immunogold electron microscopy. The antibodies attach both to a fraction of dehydrogenase-rich cytoplasmic vesicles isolated from homogenates and to vesicles of similar appearance in cells. The vesicles are filled with electron-dense material. Their tagging intensity indicates a high degree of specialization. Endometrial cells from mature animals contain a host of dehydrogenase vesicles, and cells from prepubertal animals only a few. Functional aspects of the novel organelle are discussed.
Adamski
J
J
Max-Planck-Institut für experimentelle Endokrinologie, Hannover, Germany.
Husen
B
B
Marks
F
F
Jungblut
P W
PW
D000818:Animals; D000911:Antibodies, Monoclonal; D002458:Cell Fractionation; D003102:Colloids; D004717:Endometrium / Q000201:enzymology / Q000648:ultrastructure*; D004960:Estradiol Dehydrogenases / Q000032:analysis* / Q000378:metabolism; D005260:Female; D006046:Gold; D007140:Immunoglobulin Fab Fragments; D016253:Microscopy, Immunoelectron; D015388:Organelles / Q000201:enzymology*; D013552:Swine
D000911:Antibodies, Monoclonal; D003102:Colloids; D007140:Immunoglobulin Fab Fragments; D006046:Gold; D004960:Estradiol Dehydrogenases