Functional analysis of the human neurofilament light chain gene promoter.
We have carried out a structural and functional analysis on the human NF-L (H-NF-L) gene. It contains a methylation-free island, spanning the 5' flanking sequences and the first exon and a number of neuronal-specific DNase I hypersensitive sites have been identified in the upstream region as well as within the body of the gene. Analysis in cell lines and transgenic mice using a combination of these sites has revealed the presence of a conserved element(s) between -300bp and -190bp which is required for neuronal-specific expression.
Yazdanbakhsh
K
K
National Institute for Medical Research, Mill Hill, London, UK.
Fraser
P
P
Kioussis
D
D
Vidal
M
M
Grosveld
F
F
Lindenbaum
M
M
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002843:Chromatin / Q000648:ultrastructure; D004247:DNA; D003850:Deoxyribonuclease I; D015870:Gene Expression; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D016900:Neurofilament Proteins / Q000235:genetics* / Q000378:metabolism; D011401:Promoter Regions, Genetic; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D014162:Transfection
D002843:Chromatin; D016900:Neurofilament Proteins; C044882:neurofilament protein L; D004247:DNA; D003850:Deoxyribonuclease I