title: Cloning and sequence analysis of the coat protein gene of barley mild mosaic virus.
abstract: The sequence of the 3' 1462nts of RNA-1 of a UK isolate of the fungal-transmitted virus barley mild mosaic (BaMMV) has been determined. An open reading frame encoding the coat protein gene was identified within this region using amino acid sequence information obtained by cyanogen bromide cleavage of virus particles. The amino acid sequence of the full-length coat protein was deduced from the nucleotide sequence. Amino acid sequence comparisons revealed highest homology to the coat protein of barley yellow mosaic virus. In addition, a significant, but limited, number of the amino acid residues that are conserved between aphid-transmitted potyviruses were also conserved between BaMMV and potyviruses.
authors.affiliation: Plant Breeding International, Trumpington, Cambridge, UK.
authors.forename: I J
authors.initials: IJ
authors.lastname: Foulds
authors.forename: V J
authors.initials: VJ
authors.lastname: Lea
authors.forename: C
authors.initials: C
authors.lastname: Sidebottom
authors.forename: C M
authors.initials: CM
authors.lastname: James
authors.forename: R E
authors.initials: RE
authors.lastname: Boulton
authors.forename: T
authors.initials: T
authors.lastname: Brears
authors.forename: A R
authors.initials: AR
authors.lastname: Slabas
authors.forename: P L
authors.initials: PL
authors.lastname: Jack
authors.forename: R
authors.initials: R
authors.lastname: Stratford
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002213:Capsid / Q000235:genetics; D003001:Cloning, Molecular; D005814:Genes, Viral; D001467:Hordeum / Q000382:microbiology; D008969:Molecular Sequence Data; D009029:Mosaic Viruses / Q000145:classification / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics; D017423:Sequence Analysis, RNA; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
chemical_list: D012367:RNA, Viral