title: A conformation-dependent monoclonal antibody against active chicken acetylcholinesterase.
abstract: We show that the C-131 monoclonal antibody, directed against chicken AChE, recognizes active chicken AChE, but not the SDS-denatured or heat-inactivated protein. Previous results indicated that C-131 only binds to the active enzyme, and not to inactive molecules which also occur in the embryonic chicken brain. In contrast with C-131, other monoclonal antibodies obtained in the same series, such as C-6 and C-54, also recognize denatured or inactive AChE. It is noteworthy that these antibodies all seem to react with a trypsin-sensitive peptide which is present in chicken but not in mammalian or Torpedo AChE, whereas the C-131 antibody binds trypsin-modified as well as intact molecules. These results show that C-131 is highly conformation-dependent, specific for active AChE. They confirm our previous conclusion that active and inactive molecules arise from different folding processes.
authors.affiliation: Laboratoire de Neurobiologie, CNRS URA 295, Ecole Normale Supérieure, Paris, France.
authors.forename: J M
authors.initials: JM
authors.lastname: Chatel
authors.forename: F M
authors.initials: FM
authors.lastname: Vallette
authors.forename: J
authors.initials: J
authors.lastname: Massoulié
authors.forename: J
authors.initials: J
authors.lastname: Grassi
mesh_terms: D000110:Acetylcholinesterase / Q000737:chemistry* / Q000276:immunology*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D015153:Blotting, Western; D002645:Chickens; D006358:Hot Temperature; D011487:Protein Conformation; D011489:Protein Denaturation; D014357:Trypsin / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D000110:Acetylcholinesterase; D014357:Trypsin