title: A designed metal-binding protein with a novel fold.
abstract: A major challenge in protein design is to create stable scaffolds into which tailored functions can be introduced. Here we present the design, synthesis and characterization of a 61-residue all-beta protein: the minibody. We used a portion of the heavy chain variable domain of an immunoglobulin as a template, obtaining a molecule with a novel beta-sheet scaffold and two regions corresponding to the hypervariable loops H1 and H2. To exploit the potential for creating functional centres in the minibody, we engineered a metal-binding site into it. This site is formed by one histidine in H1 and two in H2. The protein is folded, compact and able to bind metal, thus representing the first designed beta-protein with a novel fold and a tailored function. By randomizing the sequence of the hypervariable loops, we are using the minibody scaffold to construct a conformationally constrained peptide library displayed on phage.
authors.affiliation: Department of Biochemistry, Istituto di Ricerche di Biologia Molecolare P. Angeletti IRBM, Roma, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Pessi
authors.forename: E
authors.initials: E
authors.lastname: Bianchi
authors.forename: A
authors.initials: A
authors.lastname: Crameri
authors.forename: S
authors.initials: S
authors.lastname: Venturini
authors.forename: A
authors.initials: A
authors.lastname: Tramontano
authors.forename: M
authors.initials: M
authors.lastname: Sollazzo
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D006639:Histidine; D007143:Immunoglobulin Heavy Chains / Q000737:chemistry; D007135:Immunoglobulin Variable Region / Q000737:chemistry; D008670:Metals / Q000378:metabolism*; D008969:Molecular Sequence Data; D016296:Mutagenesis; D015202:Protein Engineering; D017510:Protein Folding
chemical_list: D007143:Immunoglobulin Heavy Chains; D007135:Immunoglobulin Variable Region; D008670:Metals; D006639:Histidine