title: Efficient random mutagenesis method with adjustable mutation frequency by use of PCR and dITP.
authors.affiliation: Department of Biophysical Chemistry, Netherlands Institute for Dairy Research (NIZO), Ede.
authors.forename: J H
authors.initials: JH
authors.lastname: Spee
authors.forename: W M
authors.initials: WM
authors.lastname: de Vos
authors.forename: O P
authors.initials: OP
authors.lastname: Kuipers
mesh_terms: D015544:Inositol 1,4,5-Trisphosphate / Q000031:analogs & derivatives*; D016296:Mutagenesis; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: C067150:6-deoxyinositol 1,4,5-triphosphate; D015544:Inositol 1,4,5-Trisphosphate