Mitochondrial DNA deletion in human myocardium.
Mutation of myocardial mitochondrial DNA was investigated in human left ventricles obtained at autopsy using the polymerase chain reaction (PCR). Seventeen autopsy cases were examined, including patients with diabetes mellitus, myocardial infarction, cardiomyopathy, cancer, and other diseases. Two cases of diabetes mellitus, 2 of myocardial infarction, and 1 of pulmonary fibrosis showed a 7.4 kb deletion of myocardial mitochondrial DNA. Primer shift PCR confirmed that an amplified DNA fragment had not been obtained by misannealing of the primers. It is unclear how much these findings are related to the severity or prognosis of the various diseases, but they indicate that mutation of myocardial mitochondrial DNA can occur in other diseases besides cardiomyopathy, although the influence of aging could not be excluded.
Takeda
N
N
Department of Internal Medicine, Aoto Hospital, Jikei University School of Medicine, Tokyo, Japan.
Tanamura
A
A
Iwai
T
T
Nakamura
I
I
Kato
M
M
Ohkubo
T
T
Noma
K
K
D000375:Aging; D001483:Base Sequence; D009202:Cardiomyopathies / Q000378:metabolism; D004249:DNA Damage; D004272:DNA, Mitochondrial / Q000737:chemistry*; D003920:Diabetes Mellitus / Q000378:metabolism; D005260:Female; D006352:Heart Ventricles / Q000737:chemistry; D006801:Humans; D008297:Male; D008929:Mitochondria, Heart / Q000378:metabolism*; D008969:Molecular Sequence Data; D009203:Myocardial Infarction / Q000378:metabolism; D016133:Polymerase Chain Reaction
D004272:DNA, Mitochondrial