c-fos oncogene underexpression in salivary gland tumors as measured by in situ hybridization.
Tissue from 35 salivary gland tumors and 14 normal salivary glands was analyzed by in situ hybridization and computer-assisted morphometry for the expression of the c-fos oncogene. The normal salivary gland tissues were found to express c-fos focally, mainly in the acinar secretory cells. The majority of the cells in the normal tissues showed a high level of expression (47.74 +/- 5.31% of cells had 46 to 60 grains per cell and another 45.79 +/- 2.18% showed > 60 grains per cell). All the tumors examined exhibited a relatively low, uniform distribution of c-fos expression. For example, in the poorly differentiated adenocarcinomas, 96.83 +/- 04% of the cells were found to have < 15 grains per cell. A general linear model for multivariate analysis showed a significant difference between the various tumor types and the normal salivary gland tissues (P = 0.0001). These data support the hypothesis that salivary gland tumors belong to a group of epithelial neoplasias in which the loss of cellular differentiation is linked with underexpression of the c-fos oncogene.
Birek
C
C
Department of Oral Pathology, Faculty of Dentistry, University of Toronto, Ontario, Canada.
Lui
E
E
Dardick
I
I
D001345:Autoradiography; D015870:Gene Expression; D016762:Genes, fos; D006801:Humans; D007091:Image Processing, Computer-Assisted; D017403:In Situ Hybridization; D015999:Multivariate Analysis; D012016:Reference Values; D012468:Salivary Gland Neoplasms / Q000235:genetics* / Q000473:pathology; D012469:Salivary Glands / Q000503:physiopathology