The protein core of the NCA-related pancreatic adenocarcinoma-associated antigen (DD9-Ag) is NCA-50.
Earlier studies with monoclonal antibody (mcAb) DD9E7 have identified a family of nonspecific cross-reacting antigen (NCA)-related antigens that are highly expressed in human pancreatic adenocarcinoma. To analyse the molecular nature of these glycoproteins further, a lambda gt11 expression library has been constructed from the GER pancreatic adenocarcinoma cell line and screened with an NCA sequence-specific oligonucleotide probe. Sequence analysis and restriction enzyme mapping of the clones isolated have shown that they are all homologous with the NCA-50 protein core sequence rather than other related members of the carcinoembryonic antigen/NCA gene family. With use of the same probe, a 2.5-kb mRNA transcript, characteristic of NCA-50, was found in pancreatic adenocarcinoma tumour samples. This study suggests that the 80- to 115-kDa glycoproteins, which we had previously identified in pancreatic adenocarcinomas with NCA-specific mcAb DD9E7, are the result of aberrant glycosylation of the NCA-50 protein core following cell transformation.
Flomen
R
R
Department of Surgery, St. George's Hospital Medical School, London, England.
Moss
M
M
Grant
D
D
Grant
A
A
D000230:Adenocarcinoma / Q000235:genetics / Q000276:immunology*; D000595:Amino Acid Sequence; D000951:Antigens, Neoplasm / Q000032:analysis* / Q000235:genetics; D001483:Base Sequence; D015815:Cell Adhesion Molecules; D006031:Glycosylation; D006801:Humans; D008562:Membrane Glycoproteins / Q000032:analysis*; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000032:analysis*; D010190:Pancreatic Neoplasms / Q000235:genetics / Q000276:immunology*; D012689:Sequence Homology, Nucleic Acid
D000951:Antigens, Neoplasm; D015815:Cell Adhesion Molecules; D008562:Membrane Glycoproteins; D009363:Neoplasm Proteins