Study on embryo-foetotoxicity of beta-myrcene in the rat.
beta-Myrcene is a constituent of many essential oils that have been used extensively in cosmetic fragrances and as flavouring additives in the food industry. Recently, this monoterpene was reported to be an analgesic substance. Notwithstanding the widespread use of myrcene and essential oils containing myrcene in perfume and in food additives, experimental studies on the toxicity of this substance are still scarce. This study aimed to provide data on the embryo-foetotoxic potential of beta-myrcene in the rat. beta-Myrcene (0.25, 0.5 and 1.2 g/kg) in corn oil was given orally to Wistar rats from day 6 to 15 of pregnancy. Caesarean sections were performed on day 20 of pregnancy, and the number of resorptions and implantation sites were recorded. Foetuses were weighed, examined for external malformations, and fixed for visceral examination, or cleared and stained with Alizarin Red S for skeleton evaluation. No adverse effects were seen with the two lowest doses tested. Decreased weight gain during the first days of treatment and the death of one of 29 treated dams indicated that the highest dose tested (1.2 g/kg) induced maternal toxicity. A higher incidence of signs of retardation and of anomalies in the foetal skeleton indicated that 1.2 g/kg was also toxic to the rat embryo. From the data presented in this paper the no-observed-adverse-effect level for embryo-foetotoxicity could be set at 0.5 g beta-myrcene/kg body weight.
Delgado
I F
IF
Department of Pharmacology and Toxicology, National Institute of Health Quality Control, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil.
Carvalho
R R
RR
Nogueira
A C
AC
Mattos
A P
AP
Figueiredo
L H
LH
Oliveira
S H
SH
Chahoud
I
I
Paumgartten
F J
FJ
D000014:Abnormalities, Drug-Induced / Q000209:etiology*; D000080462:Acyclic Monoterpenes; D000818:Animals; D001842:Bone and Bones / Q000002:abnormalities / Q000196:embryology; D005314:Embryonic and Fetal Development / Q000187:drug effects; D005260:Female; D005503:Food Additives / Q000008:administration & dosage / Q000494:pharmacology / Q000633:toxicity*; D005865:Gestational Age; D008297:Male; D008431:Maternal-Fetal Exchange; D039821:Monoterpenes; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar; D013729:Terpenes / Q000008:administration & dosage / Q000494:pharmacology / Q000633:toxicity*
D000080462:Acyclic Monoterpenes; D005503:Food Additives; D039821:Monoterpenes; D013729:Terpenes; C008574:beta-myrcene