Human catalase is imported and assembled in peroxisomes of Saccharomyces cerevisiae.
To study the conservation of peroxisomal targeting signals, we have determined the intracellular localization of human peroxisomal catalase when expressed in yeast. Using immunofluorescence, differential centrifugation and immunoelectron microscopy, we show that the protein is targeted to the peroxisomes of the heterologous cell and assembled in its active tetrameric form. These data show the conservation of the catalase targeting signal and import specificity between human and yeast peroxisomes.
de Hoop
M J
MJ
Laboratory of Biochemistry, Groningen University, The Netherlands.
Holtman
W L
WL
Ab
G
G
D000595:Amino Acid Sequence; D002374:Catalase / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D006801:Humans; D008830:Microbodies / Q000201:enzymology*; D016253:Microscopy, Immunoelectron; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics / Q000648:ultrastructure; D014162:Transfection
D011994:Recombinant Proteins; D002374:Catalase