Skeletal muscle mitochondrial beta-oxidation of dicarboxylates.
(1) The oxidation of [U-14C]hexadecanedionoyl-mono-CoA by rat skeletal muscle mitochondrial fractions is carnitine dependent and is inhibited by cyanide. (2) [U-14C]hexadecanedionoyl-mono-CoA was oxidised at a rate 8% of that of [U-14C]hexadecanoyl-CoA. (3) Oxidations were saturable and no substrate inhibition was observed. (4) We demonstrate the formation of dicarboxylyl-mono-CoA esters and the corresponding carnitine derivatives. (5) We conclude that, although skeletal muscle mitochondria are capable of the beta-oxidation of dicarboxylic acids, this is unlikely to be of great physiological significance.
Pourfarzam
M
M
Department of Child Health, Medical School, University of Newcastle upon Tyne, UK.
Bartlett
K
K
D000214:Acyl Coenzyme A / Q000037:antagonists & inhibitors / Q000378:metabolism*; D000818:Animals; D002250:Carbon Radioisotopes; D002331:Carnitine / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D003486:Cyanides / Q000494:pharmacology; D008297:Male; D008929:Mitochondria, Heart / Q000378:metabolism; D008931:Mitochondria, Muscle / Q000378:metabolism*; D010084:Oxidation-Reduction; D010168:Palmitates / Q000378:metabolism; D010171:Palmitoyl Coenzyme A / Q000037:antagonists & inhibitors / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
D000214:Acyl Coenzyme A; D002250:Carbon Radioisotopes; D003486:Cyanides; D010168:Palmitates; C066987:hexadecanedionyl-mono-coenzyme A; D010171:Palmitoyl Coenzyme A; D002331:Carnitine