Cloning of two isoforms of the rat brain Na(+)-Ca2+ exchanger gene and their functional expression in HeLa cells.
Two functional isoforms of the rat brain Na(+)-Ca2+ exchanger were isolated from a lambda ZAP hippocampus cDNA library. The open reading frame of clone RBE-1 codes for a protein 935 amino acids long, and that of clone RBE-2 codes for a protein 958 amino acids long. Expression HeLa cells of Na+ gradient dependent Ca2+ transport activity was determined following transfection of the cells with either RBE-1 or RBE-2. Both clones expressed proteins that exchange Na+ with Ca2+ in an electrogenic manner and none of them exhibited a dependency of the antiport on K, since they transported Ca2+ in an Na+ gradient dependent manner in external choline chloride as well.
Furman
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I
Department of Biochemistry, Hebrew University-Hadassah Medical School, Jerusalem, Israel.
Cook
O
O
Kasir
J
J
Rahamimoff
H
H
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001923:Brain Chemistry; D002118:Calcium / Q000378:metabolism; D002352:Carrier Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D015870:Gene Expression; D006367:HeLa Cells / Q000378:metabolism*; D006624:Hippocampus / Q000737:chemistry; D006801:Humans; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D051381:Rats; D012964:Sodium / Q000378:metabolism; D019831:Sodium-Calcium Exchanger; D014162:Transfection
D002352:Carrier Proteins; D019831:Sodium-Calcium Exchanger; D004247:DNA; D012964:Sodium; D002118:Calcium