title: Evidence for an indirect effect of nerve growth factor (NGF) on the ADP-ribosylation of a 22 kDa rho-like protein in PC12 cells.
abstract: EDIN catalyzes the ADP-ribosylation of a 22 kDa protein, probably related to rho, in permeabilized PC12 cells in a time- and dose-dependent manner. Pre-treatment of the cells with nerve growth factor inhibits this EDIN-catalyzed ADP-ribosylation, both in vitro and in vivo. This inhibition is largely prevented by the addition of K-252a. Nerve growth factor itself causes neither ADP-ribosylation nor phosphorylation of this 22 kDa protein. These results suggest that the ADP-ribosylation of the rho protein is inhibited, albeit indirectly, by nerve growth factor treatment.
authors.affiliation: Section on Growth Factors, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892.
authors.forename: H
authors.initials: H
authors.lastname: Takahashi
authors.forename: G
authors.initials: G
authors.lastname: Guroff
mesh_terms: D000246:Adenosine Diphosphate Ribose / Q000378:metabolism*; D000818:Animals; D001426:Bacterial Proteins / Q000494:pharmacology; D019204:GTP-Binding Proteins / Q000737:chemistry / Q000378:metabolism*; D008970:Molecular Weight; D009414:Nerve Growth Factors / Q000494:pharmacology*; D016716:PC12 Cells
chemical_list: D001426:Bacterial Proteins; D009414:Nerve Growth Factors; C067120:epidermal cell differentiation inhibitor, Staphylococcus aureus; D000246:Adenosine Diphosphate Ribose; D019204:GTP-Binding Proteins