Localization of the CYP2D gene locus to human chromosome 22q13.1 by polymerase chain reaction, in situ hybridization, and linkage analysis.
Using a combination of somatic cell hybrids, in situ hybridization, and linkage mapping, we have been able to localize the cytochrome P450 CYP2D6 gene to chromosome 22 in the region q13.1. Linkage analysis, using locus-specific primers, showed a maximum sex-average lod score of 8.12 (theta = 0.00) between the marker pH130 (D22S64) and CYP2D6, of 6.92 (theta = 0.00) between the marker KI839 (D22S95) and CYP2D6, and of 4.80 (theta = 0.036) between the platelet-derived growth factor beta subunit gene (PDGFB) and CYP2D6.
Gough
A C
AC
Imperial Cancer Research Fund, Human Genetic Resources, Clare Hall Laboratories, Hertfordshire, United Kingdom.
Smith
C A
CA
Howell
S M
SM
Wolf
C R
CR
Bryant
S P
SP
Spurr
N K
NK
D002874:Chromosome Mapping; D002892:Chromosomes, Human, Pair 22; D019389:Cytochrome P-450 CYP2D6; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D005260:Female; D008040:Genetic Linkage; D006801:Humans; D017403:In Situ Hybridization; D008297:Male; D006899:Mixed Function Oxygenases / Q000235:genetics*; D016133:Polymerase Chain Reaction
D003577:Cytochrome P-450 Enzyme System; D006899:Mixed Function Oxygenases; D019389:Cytochrome P-450 CYP2D6