title: Transformation of Helicobacter pylori by electroporation.
abstract: A highly efficient method of introducing DNA into an important human pathogen is reported here. Electroporation-mediated transformation of a laboratory-passaged isolate of Helicobacter pylori was successfully used to establish genetic mutants at a transformation frequency of > 10(5)/micrograms DNA. This method should be widely applicable to all isolates of H. pylori and may eliminate the variability reported when natural transformation was used on fresh clinical isolates.
authors.affiliation: Department of Microbiology and Immunology, Stanford University, CA 94305.
authors.forename: E D
authors.initials: ED
authors.lastname: Segal
authors.forename: L S
authors.initials: LS
authors.lastname: Tompkins
mesh_terms: D004269:DNA, Bacterial / Q000235:genetics; D004352:Drug Resistance, Microbial / Q000235:genetics; D004560:Electricity; D016480:Helicobacter pylori / Q000235:genetics*; D009154:Mutation; D013307:Streptomycin; D014169:Transformation, Bacterial
chemical_list: D004269:DNA, Bacterial; D013307:Streptomycin