A new 'solid phase' procedure to synthesize immunotoxins (antibody-ribosome inactivating protein conjugates).
A method to produce immunotoxins (conjugates comprising of a monoclonal antibody and toxin) using ribosome inactivating protein anchored on an affinity gel derivatized with triazinic dye is described. The adsorbed toxins were activated with 2-imino-thiolane and then conjugated to monoclonal antibody activated by SPDP. The "heterogeneous phase" system offered several advantages, reducing the usually required purification steps and opening a way to automatize the conjugation procedure.
Dosio
F
F
Istituto di Chimica Farmaceutica Applicata, Torino, Italy.
Brusa
P
P
Delprino
L
L
Ceruti
M
M
Grosa
G
G
Cattel
L
L
Bolognesi
A
A
Barbieri
L
L
D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000276:immunology; D002474:Cell-Free System; D002850:Chromatography, Gel; D006801:Humans; D000922:Immunotoxins / Q000737:chemistry* / Q000633:toxicity; D010940:Plant Proteins / Q000737:chemistry / Q000276:immunology; D014176:Protein Biosynthesis; D011500:Protein Synthesis Inhibitors / Q000737:chemistry / Q000276:immunology; D054789:Ribosome Inactivating Proteins, Type 1; D014407:Tumor Cells, Cultured / Q000187:drug effects
D000911:Antibodies, Monoclonal; D000922:Immunotoxins; D010940:Plant Proteins; D011500:Protein Synthesis Inhibitors; D054789:Ribosome Inactivating Proteins, Type 1; C025674:GEL protein, Gelonium multiflorum