title: Membrane topology of MalG, an inner membrane protein from the maltose transport system of Escherichia coli.
abstract: In Escherichia coli, the binding protein-dependent transport system for maltose and maltodextrins is composed of five proteins--LamB, MalE, MalF, MalG and MalK--located in the three layers of the bacterial envelope. Proteins MalF and MalG are hydrophobic inner membrane components mediating the energy-dependent translocation of substrates into the cytoplasm. In this paper, we analyse the topology of the MalG protein by using methods based on the properties of fusions between malG and 'phoA, a truncated gene encoding alkaline phosphatase lacking its translation initiation and exportation signals. Fusions were obtained by using either phage lambda TnphoA or by constructing in vitro fusions located randomly within the malG gene. The deduced topological model suggests that MalG spans the membrane six times and has its amino- and carboxy-termini in the cytoplasm. These results will be helpful for the interpretation of the phenotypes of mutants in malG.
authors.affiliation: Unité de Programmation Moléculaire et de Toxicologie Génétique, CNRS UA 1444, Institut Pasteur, Paris, France.
authors.forename: E
authors.initials: E
authors.lastname: Dassa
authors.forename: S
authors.initials: S
authors.lastname: Muir
mesh_terms: D018528:ATP-Binding Cassette Transporters; D000465:Algorithms; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D001693:Biological Transport, Active; D002352:Carrier Proteins / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D029968:Escherichia coli Proteins; D008320:Maltose / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D011487:Protein Conformation; D011993:Recombinant Fusion Proteins / Q000378:metabolism
chemical_list: D018528:ATP-Binding Cassette Transporters; D001426:Bacterial Proteins; D002352:Carrier Proteins; D029968:Escherichia coli Proteins; C079123:MalG protein, E coli; D008565:Membrane Proteins; D011993:Recombinant Fusion Proteins; D008320:Maltose