title: T cell receptor selection by and recognition of two class I major histocompatibility complex-restricted antigenic peptides that differ at a single position.
abstract: Peptides derived from HLA-Cw3 and HLA-A24 within region 170-179 differ by a single substitution, at position 173, and are both presented by the class I major histocompatibility complex molecule H-2Kd for recognition by murine cytolytic T lymphocytes (CTLs). As a first approach to understand the way T cell receptors (TCRs) intact with the HLA peptides, we have analyzed the TCR selection by, and recognition of, the two HLA antigenic sites. First, we have compared the TCR repertoires selected by HLA-Cw3 and HLA-A24, not only by sequencing the TCRs carried by CTL clones isolated and grown in vitro, but also by analyzing the TCRs expressed in vivo by peritoneal exudate lymphocytes from immune animals. Second, we have compared the TCR crossrecognition of HLA-A24 by CTLs selected by HLA-Cw3 with that of HLA-Cw3 by CTLs selected by HLA-A24. The combined analysis of TCR selection by and recognition of these two related HLA antigenic sites provides evidence that the TCR beta junctional regions interact with the amino-terminal part of the HLA peptides.
authors.affiliation: Ludwig Institute for Cancer Research, Lausanne Branch, Epalinges, Switzerland.
authors.forename: J L
authors.initials: JL
authors.lastname: Casanova
authors.forename: F
authors.initials: F
authors.lastname: Martinon
authors.forename: H
authors.initials: H
authors.lastname: Gournier
authors.forename: C
authors.initials: C
authors.lastname: Barra
authors.forename: C
authors.initials: C
authors.lastname: Pannetier
authors.forename: A
authors.initials: A
authors.lastname: Regnault
authors.forename: P
authors.initials: P
authors.lastname: Kourilsky
authors.forename: J C
authors.initials: JC
authors.lastname: Cerottini
authors.forename: J L
authors.initials: JL
authors.lastname: Maryanski
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D004247:DNA / Q000032:analysis / Q000235:genetics; D015234:HLA-A Antigens / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D059871:HLA-A24 Antigen; D015236:HLA-C Antigens / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D015395:Histocompatibility Antigens Class I / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D008285:Major Histocompatibility Complex / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008969:Molecular Sequence Data; D010537:Peritoneum / Q000166:cytology; D016133:Polymerase Chain Reaction; D011948:Receptors, Antigen, T-Cell / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000032:analysis / Q000235:genetics / Q000276:immunology; D013601:T-Lymphocytes / Q000737:chemistry / Q000276:immunology / Q000648:ultrastructure
chemical_list: D015234:HLA-A Antigens; D059871:HLA-A24 Antigen; D015236:HLA-C Antigens; C038653:HLA-C*03 antigen; D015395:Histocompatibility Antigens Class I; D011948:Receptors, Antigen, T-Cell; D016693:Receptors, Antigen, T-Cell, alpha-beta; D004247:DNA