Covalently immobilized DNA plate for luminometric DNA-DNA hybridization to identify viridans streptococci in under 2 hours.
Single-stranded chromosomal DNA was covalently bound to a microdilution plate and used for quantitative DNA-DNA hybridization. After 30 min, hybridized DNA was quantitatively detected by alkaline phosphatase and a chemiluminescent substrate. This method was successfully used for the rapid identification of viridans streptococci.
Adnan
S
S
Department of Microbiology, Gifu University, School of Medicine, Japan.
Li
N
N
Miura
H
H
Hashimoto
Y
Y
Yamamoto
H
H
Ezaki
T
T
D004269:DNA, Bacterial / Q000032:analysis*; D008163:Luminescent Measurements; D009693:Nucleic Acid Hybridization / Q000379:methods*; D013291:Streptococcus / Q000235:genetics* / Q000302:isolation & purification
D004269:DNA, Bacterial