title: Chromosome organization of Streptococcus mutans GS-5.
abstract: Twenty-eight genetic loci have been physically mapped to specific large restriction fragments of the Streptococcus mutans GS-5 chromosome by hybridization with probes of cloned genes or, for transposon-generated amino acid auxotrophs, with probes for Tn916. In addition, restriction fragments generated by one low-frequency-cleavage enzyme were used as probes to identify overlapping fragments generated by other restriction enzymes. The approach allowed construction of a low resolution physical map of the S. mutans GS-5 genome using restriction enzymes ApaI (5'-GGGCC/C), SmaI (5'-CCC/GGG), and NotI (5'-GC/GGCCGC).
authors.affiliation: Department of Microbiology and Immunology, Temple University School of Medicine, Philadelphia, Pennsylvania 19140.
authors.forename: M J
authors.initials: MJ
authors.lastname: Hantman
authors.forename: S
authors.initials: S
authors.lastname: Sun
authors.forename: P J
authors.initials: PJ
authors.lastname: Piggot
authors.forename: L
authors.initials: L
authors.lastname: Daneo-Moore
mesh_terms: D015139:Blotting, Southern; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D016521:Electrophoresis, Gel, Pulsed-Field; D016680:Genome, Bacterial; D016254:Mutagenesis, Insertional; D009693:Nucleic Acid Hybridization; D015183:Restriction Mapping; D013295:Streptococcus mutans / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial; C025651:CCCGGG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific; C038565:GCGGCCGC-specific type II deoxyribonucleases; C038485:GGGCCC-specific type II deoxyribonucleases