SP6 RNA polymerase containing vaccinia virus for rapid expression of cloned genes in tissue culture.
A hybrid transient expression system, in which tissue culture cells are infected with a recombinant vaccinia virus encoding bacteriophage DNA-dependent RNA polymerase and transfected with a plasmid containing a cloned gene behind the bacteriophage promoter, allows rapid high-level expression in nearly 100% of the cells. In order to extend this system to clones from libraries containing SP6 promoters, a new vaccinia virus was constructed encoding bacteriophage SP6 RNA polymerase.
Usdin
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TB
Natl. Institute of Mental Health, National Institutes of Health, Bethesda, MD 20892.
Brownstein
M J
MJ
Moss
B
B
Isaacs
S N
SN
D001435:Bacteriophages / Q000201:enzymology*; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D015870:Gene Expression; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins; D014162:Transfection; D014616:Vaccinia virus / Q000201:enzymology / Q000235:genetics*; D001616:beta-Galactosidase / Q000235:genetics
D004279:DNA, Viral; D011994:Recombinant Proteins; C070852:RNA polymerase SP6; D012321:DNA-Directed RNA Polymerases; D001616:beta-Galactosidase