Modulation of the heat shock response by one-carbon metabolism in Escherichia coli.
A genetic screen designed to isolate mutants of Escherichia coli W3110 altered in the ability to induce the heat shock response identified a strain unable to induce the heat shock proteins in a rich, defined medium lacking methionine after exposure to 2,4-dinitrophenol. This strain also grew slowly at 28 degrees C and linearly at 42 degrees C in this medium. The abnormal induction of the heat shock proteins and abnormal growth at both high and low temperatures were reversed when methionine was included in the growth medium. The mutation responsible for these phenotypes mapped to the glyA gene, a biosynthetic gene encoding the enzyme that converts serine and tetrahydrofolate to glycine and 5,10-methylenetetrahydrofolate. This reaction is the major source of glycine and one-carbon units in the cell. Because fixed one-carbon units, in the form of methionine, allowed mutant cells to induce the heat shock response after exposure to 2,4-dinitrophenol, a one-carbon restriction may be responsible for the phenotypes described above.
Gage
D J
DJ
Program in Cellular and Molecular Biology, University of Michigan Medical School, Ann Arbor 48109-0620.
Neidhardt
F C
FC
D019297:2,4-Dinitrophenol; D000220:Adaptation, Biological; D000483:Alleles; D003001:Cloning, Molecular; D004140:Dinitrophenols / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D005798:Genes, Bacterial / Q000235:genetics; D005816:Genetic Complementation Test; D005998:Glycine / Q000378:metabolism; D012696:Glycine Hydroxymethyltransferase / Q000235:genetics*; D006360:Heat-Shock Proteins / Q000096:biosynthesis*; D006358:Hot Temperature; D008715:Methionine / Q000378:metabolism; D016296:Mutagenesis; D010641:Phenotype; D012694:Serine / Q000378:metabolism; D013763:Tetrahydrofolates / Q000378:metabolism
D004140:Dinitrophenols; D006360:Heat-Shock Proteins; D013763:Tetrahydrofolates; C013123:5,10-methylenetetrahydrofolic acid; C030371:5,6,7,8-tetrahydrofolic acid; D012694:Serine; D008715:Methionine; D012696:Glycine Hydroxymethyltransferase; D019297:2,4-Dinitrophenol; D005998:Glycine