title: Genetic susceptibility to the induction of murine experimental autoimmune orchitis (EAO) without adjuvant. I. Comparison of pathology, delayed type hypersensitivity, and antibody.
abstract: In the present study, it was demonstrated that there were marked strain differences in susceptibility to the induction of our new murine model of experimental autoimmune orchitis (EAO; definite orchitis with hypospermatogenesis) induced by two or three sc injections with viable syngeneic testicular germ cells (TC) without any adjuvants. Among 12 inbred strains of mice examined, the A/J (H-2a), C3H/He (H-2k), and C3H/HeN (H-2k) strains were highly susceptible, whereas the C57BL/6N (H-2b), C57BL/10Sn (H-2b), BALB/cAnN (H-2d), AKR/N (H-2k), CBA/JN (H-2k), C3H/HeJ (H-2k), and MRL/lpr (H-2k) strains were low susceptible, and the DBA/2N (H-2d) as well as C3H/BiKi (H-2k) strains were resistant. In particular, mice of the H-2k haplotype demonstrated varying degrees of susceptibility, from highly to totally resistant, to the induction of EAO. Disease susceptibility to this type of EAO does not seem to be associated with a particular H-2 haplotype. All mice of the highly susceptible strains that received two injections of TC (TC x 2) developed a significant increase in both levels of delayed footpad reaction (DFR) to TC and anti-TC antibodies measured by ELISA. In the low susceptible and the resistant strains receiving TC x 2 or TC x 3, there was no correlation between the immune responses and the susceptibility to disease in these strains, with the exception of the BALB/cAnN mice receiving TC x 3. The low susceptible and the resistant mice that received TC x 2 were classified into four groups based on the DFR and antibody response: the C57BL/6N, BALB/cAnN, CBA/JN, and C3H/HeJ strains were both positive, and the C57BL/10Sn and AKR/N strains were both negative or very low; the DBA/2N and MRL/lpr strains showed negative DFR and positive antibody response, and the C3H/BiKi strain showed quite the opposite. Almost all mice of the 12 inbred strains that received TC x 3 showed positive antibody response, although its level varied. There seems to be no linkage between the cell-mediated and humoral immune responses and the H-2 locus in our new EAO model.
authors.affiliation: Department of Immunology and Immunopathology, Kagawa Medical School, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Tokunaga
authors.forename: C
authors.initials: C
authors.lastname: Hiramine
authors.forename: M
authors.initials: M
authors.lastname: Itoh
authors.forename: A
authors.initials: A
authors.lastname: Mukasa
authors.forename: K
authors.initials: K
authors.lastname: Hojo
mesh_terms: D000818:Animals; D000917:Antibody Formation; D001327:Autoimmune Diseases / Q000209:etiology*; D004195:Disease Models, Animal; D020022:Genetic Predisposition to Disease; D006968:Hypersensitivity, Delayed / Q000276:immunology*; D007114:Immunization; D016219:Immunotherapy, Adoptive; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains / Q000235:genetics*; D009920:Orchitis / Q000276:immunology* / Q000473:pathology; D013094:Spermatozoa / Q000166:cytology / Q000276:immunology