A progress report on the crystallographic studies on the B800-850 antenna complex from Rhodopseudomonas acidophila strain 10050.
Large single crystals (up to 1 mm in each dimension) of the B800-850 antenna complex from Rhodopseudomonas acidophila strain 10050 have been grown in the presence of beta-octyl-glucoside. These crystals have the space group R32 and unit cell dimensions of a = b = 119.9 A and c = 297.0 A. Recently we have improved our crystallization procedures so that all crystals now diffract reliably to beyond 3.5 A, with some diffracting to below 3 A. A range of isomorphous heavy atom derivatives have been prepared and we are now engaged in locating the heavy atom sites within the unit cell.
Cogdell
R J
RJ
Department of Botany, University of Glasgow.
Halloren
E
E
Hawthornthwaite
A M
AM
Thunnissen
M M
MM
Freer
A A
AA
McDermott
G
G
Guthrie
N
N
Isaacs
N
N
Lindsay
J G
JG
Papiz
M
M
D001059:Apoproteins / Q000737:chemistry; D001426:Bacterial Proteins / Q000737:chemistry* / Q000302:isolation & purification; D001429:Bacteriochlorophylls / Q000737:chemistry; D003460:Crystallization; D012241:Rhodopseudomonas / Q000737:chemistry*; D014961:X-Ray Diffraction
D001059:Apoproteins; D001426:Bacterial Proteins; D001429:Bacteriochlorophylls