title: Increased expression of PDGF A- and B-chain genes in rat lungs with hypoxic pulmonary hypertension.
abstract: To study the molecular basis of vascular remodeling in pulmonary hypertension, we developed an experimental system in which male Sprague-Dawley rats were exposed to hypoxia for up to 3 wk. Both the right ventricular systolic pressure and gravimetric index for right ventricular hypertrophy were higher in rats exposed to hypoxia for 3 wk than those of age-matched control rats (P < 0.01), indicating that pulmonary hypertension was established under conditions used. To examine the possible involvement of platelet-derived growth factor (PDGF) in the pulmonary vascular remodeling caused by hypoxia, we cloned rat PDGF A- and B-chain cDNA and prepared specific cRNA probes. Northern blot analysis revealed that PDGF B-chain mRNA levels in the lungs were increased, reached a maximum of day 1, and were sustained at day 3, whereas PDGF A-chain mRNA levels reached a maximum on day 3. Thus the increase in the PDGF B-chain mRNA level precedes that in the PDGF A-chain mRNA level. These results suggest that the PDGF A- and B-chain products may be coordinately and sequentially involved in hypoxic pulmonary vascular remodeling.
authors.affiliation: First Department of Internal Medicine, Tohoku University School of Medicine, Miyagi, Japan.
authors.forename: D
authors.initials: D
authors.lastname: Katayose
authors.forename: M
authors.initials: M
authors.lastname: Ohe
authors.forename: K
authors.initials: K
authors.lastname: Yamauchi
authors.forename: M
authors.initials: M
authors.lastname: Ogata
authors.forename: K
authors.initials: K
authors.lastname: Shirato
authors.forename: H
authors.initials: H
authors.lastname: Fujita
authors.forename: S
authors.initials: S
authors.lastname: Shibahara
authors.forename: T
authors.initials: T
authors.lastname: Takishima
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D015870:Gene Expression; D006439:Hemodynamics; D006976:Hypertension, Pulmonary / Q000209:etiology* / Q000235:genetics* / Q000503:physiopathology; D000860:Hypoxia / Q000150:complications* / Q000235:genetics; D008168:Lung / Q000502:physiology*; D008297:Male; D015335:Molecular Probes / Q000235:genetics; D008969:Molecular Sequence Data; D010982:Platelet-Derived Growth Factor / Q000235:genetics*; D016133:Polymerase Chain Reaction; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D015335:Molecular Probes; D010982:Platelet-Derived Growth Factor; C075278:platelet-derived growth factor A; D004247:DNA