title: Acute effects of ethanol on pattern reversal and flash-evoked potentials in rats and the relationship to body temperature.
abstract: The effects of acute ethanol treatment on flash and pattern reversal visual evoked potentials (FEPs and PREPs, respectively) were examined in three experiments using Long-Evans rats. The relationships of evoked potential parameters with blood ethanol concentration and body temperature were examined. In Experiment 1, rats were treated i.p. with vehicle or 0.5, 1.0 or 2.0 g ethanol/kg body weight, and tested 30 min later. The 2.0 g/kg group had prolonged latencies of PREP peaks, no changes in PREP peak-to-peak amplitudes, and lower body temperatures than saline-treated controls. The peak latency shifts were significantly correlated with both blood ethanol concentration and body temperature, and were of a magnitude to be expected from similar changes in body temperature alone. Experiment 2 measured both PREPs and paired-flash FEPs in rats 30 min after injection of either 0, 0.5 or 2.0 g/kg ethanol. PREP changes were found following treatment with the high dose which were similar to those of Experiment 1. Some FEP peak latencies were prolonged and peak-to-peak amplitudes were reduced by both doses of ethanol, despite the fact that body temperatures were reduced at only the high dose. At 2.0 g/kg ethanol, the FEP changes in latency, but not amplitude, were in accordance with what would be expected from body temperature changes alone. The third study attempted to investigate the role of reduced body temperature in producing the visual evoked potential changes by testing at room temperatures of 22 or 30 degrees C. Contrary to expectations, the rats receiving 2 g/kg ethanol were approx. 1 degree C cooler than controls at both room temperatures. Evoked potential latencies were greater in ethanol-treated rats than controls at both room temperatures. There were no significant effects of ethanol on FEP amplitudes. Overall, the effects of low doses of ethanol were independent of temperature changes, but the effects of higher doses of ethanol (2.0 g/kg) could not be distinguished from those produced by differences in body temperature alone.
authors.affiliation: Neurotoxicology Division, U.S. Environmental Protection Agency, Research Triangle Park, NC 27711.
authors.forename: W K
authors.initials: WK
authors.lastname: Boyes
authors.forename: B E
authors.initials: BE
authors.lastname: Hetzler
authors.forename: R S
authors.initials: RS
authors.lastname: Dyer
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001831:Body Temperature / Q000187:drug effects* / Q000502:physiology; D000431:Ethanol / Q000494:pharmacology*; D005074:Evoked Potentials, Visual / Q000187:drug effects* / Q000502:physiology; D008297:Male; D010364:Pattern Recognition, Visual / Q000502:physiology*; D010775:Photic Stimulation; D051381:Rats; D011930:Reaction Time / Q000187:drug effects / Q000502:physiology
chemical_list: D000431:Ethanol