Rapid polymerase chain reaction method for detection of Vibrio cholerae in foods.
The polymerase chain reaction was used to selectively amplify sequences within the cholera toxin operon from Vibrio cholerae O1. Oysters, crabmeat, shrimp, and lettuce were seeded with V. cholerae and then homogenized or washed with alkaline peptone water, followed by short-term (6- to 8-h) enrichment. A detection limit of as few as 1 V. cholerae CFU per 10 g of food was obtained with amplification reactions from crude bacterial lysates. The method is extremely rapid and obviates the need for DNA isolation from a variety of complex food matrices.
Koch
W H
WH
Division of Microbiology, Food and Drug Administration, Washington, D.C. 20204.
Payne
W L
WL
Wentz
B A
BA
Cebula
T A
TA
D000818:Animals; D001483:Base Sequence; D002772:Cholera Toxin / Q000032:analysis*; D005516:Food Microbiology; D005638:Fruit; D005798:Genes, Bacterial / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D012758:Shellfish; D014675:Vegetables; D014734:Vibrio cholerae / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*
D002772:Cholera Toxin