High-performance liquid chromatographic measurement of leucine and alpha-ketoisocaproate in whole blood: application to fetal protein metabolism.
Evaluation of fetal protein metabolism requires measurement of a number of variables including umbilical blood flow, CO2 radioactivity, as well as plasma specific activities, whole blood concentration, and radioactivity of leucine and alpha-ketoisocaproate. This report details methods of analysis for whole blood concentration and radioactivity of leucine and alpha-ketoisocaproate using high-performance liquid chromatography that can be done on minimal blood volumes and are sufficiently accurate to detect the small arteriovenous differences important in measurements of fetal metabolism. Using these methods, the important components of fetal protein metabolism such as protein synthesis can be calculated with sufficient accuracy to detect differences as small as 10% provided appropriate experimental designs are used.
Milley
J R
JR
Department of Pediatrics, University of Utah School of Medicine, Salt Lake City 84132.
Sweeley
J C
JC
D000818:Animals; D000983:Antipyrine / Q000097:blood; D002245:Carbon Dioxide / Q000097:blood; D002851:Chromatography, High Pressure Liquid; D005260:Female; D005326:Fetal Proteins / Q000378:metabolism*; D007651:Keto Acids / Q000097:blood*; D007930:Leucine / Q000097:blood*; D011247:Pregnancy; D011874:Radiometry; D012756:Sheep; D013056:Spectrophotometry, Ultraviolet
D005326:Fetal Proteins; D007651:Keto Acids; D002245:Carbon Dioxide; C013082:alpha-ketoisocaproic acid; D007930:Leucine; D000983:Antipyrine