title: Oxidation of low-density lipoproteins: intraindividual variability and the effect of dietary linoleate supplementation.
abstract: Low-density lipoprotein (LDL) oxidation was measured in vitro to determine intraindividual variability and to relate oxidation to linoleic acid enrichment. Intraindividual variability was determined for eight subjects on 3 consecutive days after 14 d on a fixed diet. Coefficients of variation were 7.49 +/- 1.50%, 6.58 +/- 1.16%, and 4.58 +/- 0.77% for oxidation rate, lag time, and diene concentration, respectively. In the second study 12 normolipidemic men consumed a daily diet supplement containing 35 g linoleate-rich oil in one period and 35 g oleate-rich oil in the other period (2 x 3 wk crossover). LDL oxidized faster after the linoleate diet than after the oleate diet (mean +/- SE: 16.42 +/- 0.85 and 13.16 +/- 0.68 nmol diene.mg LDL protein-1.min-1, respectively, P < 0.02) and produced more conjugated diene (416 +/- 12.60 and 379.29 +/- 11.06 nmol/mg protein, respectively, P < 0.05) in proportion to the increase in LDL linoleate (r = 0.698, P < 0.001 and r = 0.618, P < 0.01 for rate and diene concentration, respectively). Lag time before onset of oxidation was not significantly altered by the linoleate-rich diet.
authors.affiliation: Commonwealth Scientific and Industrial Research Organisation (CSIRO), Division of Human Nutrition, Adelaide, Australia.
authors.forename: M
authors.initials: M
authors.lastname: Abbey
authors.forename: G B
authors.initials: GB
authors.lastname: Belling
authors.forename: M
authors.initials: M
authors.lastname: Noakes
authors.forename: F
authors.initials: F
authors.lastname: Hirata
authors.forename: P J
authors.initials: PJ
authors.lastname: Nestel
mesh_terms: D002338:Carotenoids / Q000008:administration & dosage; D003300:Copper / Q000494:pharmacology; D019327:Copper Sulfate; D004042:Dietary Fats, Unsaturated / Q000008:administration & dosage / Q000494:pharmacology*; D002149:Energy Intake; D005227:Fatty Acids / Q000097:blood; D005229:Fatty Acids, Monounsaturated / Q000008:administration & dosage / Q000494:pharmacology; D005231:Fatty Acids, Unsaturated / Q000008:administration & dosage / Q000494:pharmacology; D005260:Female; D006801:Humans; D007700:Kinetics; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000008:administration & dosage / Q000494:pharmacology*; D008077:Lipoproteins, LDL / Q000097:blood*; D008297:Male; D019301:Oleic Acid; D009829:Oleic Acids / Q000008:administration & dosage / Q000494:pharmacology; D010084:Oxidation-Reduction; D014810:Vitamin E / Q000008:administration & dosage; D019207:beta Carotene
chemical_list: D004042:Dietary Fats, Unsaturated; D005227:Fatty Acids; D005229:Fatty Acids, Monounsaturated; D005231:Fatty Acids, Unsaturated; D008041:Linoleic Acids; D008077:Lipoproteins, LDL; D009829:Oleic Acids; D019207:beta Carotene; D014810:Vitamin E; D019301:Oleic Acid; D002338:Carotenoids; D003300:Copper; D019787:Linoleic Acid; D019327:Copper Sulfate