title: Effects of chronic total sleep deprivation on central noradrenergic receptors in rat brain.
abstract: The effect of chronic total sleep deprivation (TSD) on the regulation of central noradrenergic receptors was evaluated. Rats were subjected to 10 days of TSD by the disk-over-water method. As in previous TSD studies, these rats showed greater increases in food intake and energy expenditure and greater eventual declines in waking body temperature than their yoked-control (TSC) rats. After sacrifice, alpha 1-, alpha 2-, and beta-adrenoceptors were determined in 11 brain regions using radioligand binding assays with [3H]prazosin, [3H]rauwolscine, and 125I-iodocyanopindolol, respectively. Adrenoceptor density and affinity values were significantly different among TSD, TSC, and normally caged control rat groups only for the cerebellum, which showed higher alpha 2-binding density concomitant with lower affinity and lower beta-binding density than cage control rats. Such changes are attributable to apparatus or stimulus effects common to TSD and TSC rats. Given the absence of firm evidence for a TSD-induced downregulation of central noradrenergic receptors, the present results offer no support for the hypothesis of Siegel and Rogawski that a major function of paradoxical sleep is to upregulate these receptors.
authors.affiliation: Department of Psychiatry, University of Chicago, IL.
authors.forename: L L
authors.initials: LL
authors.lastname: Tsai
authors.forename: B M
authors.initials: BM
authors.lastname: Bergmann
authors.forename: B D
authors.initials: BD
authors.lastname: Perry
authors.forename: A
authors.initials: A
authors.lastname: Rechtschaffen
mesh_terms: D000818:Animals; D001923:Brain Chemistry / Q000502:physiology*; D004567:Electrodes, Implanted; D004569:Electroencephalography; D004576:Electromyography; D007700:Kinetics; D008297:Male; D009638:Norepinephrine / Q000502:physiology*; D011869:Radioligand Assay; D051381:Rats; D017207:Rats, Sprague-Dawley; D011941:Receptors, Adrenergic / Q000502:physiology*; D012892:Sleep Deprivation / Q000502:physiology*; D012895:Sleep, REM / Q000502:physiology
chemical_list: D011941:Receptors, Adrenergic; D009638:Norepinephrine