Selenium supplementation enhances the expression of interleukin 2 receptor subunits and internalization of interleukin 2.
Selenium (Se) is an essential nutritional factor that was shown previously by us to alter the kinetics of expression of high affinity (p55/p75) interleukin 2 receptors (IL-2R). This study shows that dietary (2 ppm for 8 weeks) or in vitro (1 x 10(-7) M) supplementation with Se (as sodium selenite) results in a significant upregulation of the expression of both the p55 and p70/75 IL-2 binding sites on the surface of concanavalin A-stimulated lymphocytes from C57BL/6J mice. This resulted in the formation of significantly higher numbers of high affinity IL-2R/cell with preservation of the normal ratio of high affinity to total IL-2 binding sites/cell. The high affinity IL-2R on cells from Se-supplemented animals functioned normally in terms of ligand binding and kinetics of IL-2 internalization, but their greater numbers/cell resulted in the internalization of significantly larger amounts of IL-2/cell. As Se supplementation results in an earlier expression of greater numbers of high affinity IL-2R, the presence of Se in the cell environment can result in an accelerated clonal expansion of activated lymphocytes.
Roy
M
M
Department of Histology, New York University Dental Center, New York 10010.
Kiremidjian-Schumacher
L
L
Wishe
H I
HI
Cohen
M W
MW
Stotzky
G
G
D000818:Animals; D001665:Binding Sites / Q000187:drug effects; D001835:Body Weight / Q000187:drug effects; D002478:Cells, Cultured; D003208:Concanavalin A; D004032:Diet; D007376:Interleukin-2 / Q000378:metabolism*; D007700:Kinetics; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D015375:Receptors, Interleukin-2 / Q000096:biosynthesis* / Q000187:drug effects; D012643:Selenium / Q000008:administration & dosage / Q000494:pharmacology*; D013997:Time Factors; D015854:Up-Regulation
D007376:Interleukin-2; D015375:Receptors, Interleukin-2; D003208:Concanavalin A; D012643:Selenium