title: Distribution of TaqI sites along human chromosomes revealed by in situ enzyme-nick translation.
abstract: We have studied the relative richness of TaqI sites along human chromosomes by means of a nonradioactive in situ enzyme-nick translation procedure. Regions with a higher content of these sequences are shown to be the noncentromeric heterochromatin blocks, whereas within euchromatin, terminal R-bands are the domains more enriched in these sites. Results obtained suggest that the method of performing enzyme digestions using time as a variable, and then in situ nick translation, provides much more complete information about the distribution of enzyme sequences along chromosomes than standard enzyme digestions.
authors.affiliation: Departamento de Biología, Unidad de Genética, Facultad de Ciencias, Universidad Autónoma de Madrid, Spain.
authors.forename: I
authors.initials: I
authors.lastname: Tagarro
authors.forename: J J
authors.initials: JJ
authors.lastname: Gonzalez-Aguilera
authors.forename: A M
authors.initials: AM
authors.lastname: Fernandez-Peralta
authors.forename: G F
authors.initials: GF
authors.lastname: de Stefano
authors.forename: L
authors.initials: L
authors.lastname: Ferrucci
mesh_terms: D001665:Binding Sites; D002871:Chromosome Banding / Q000379:methods*; D002877:Chromosomes, Human; D015252:Deoxyribonucleases, Type II Site-Specific; D006801:Humans
chemical_list: D015252:Deoxyribonucleases, Type II Site-Specific; C027046:TCGA-specific type II deoxyribonucleases