Development of a sperm zona pellucida binding assay for bull semen.
Assays based on sperm zona pellucida binding have been developed as diagnostic tests to predict the fertilisation potential of human spermatozoa. The aim of this study was to establish a similar assay for bull sperm. The results showed differences between established fertile bulls in the relative numbers of sperm cells bound to the zona pellucida of a batch of oocytes. These differences suggest that there may be a relationship between the sperm zona pellucida binding capacity and the fertility of bulls.
Fazeli
A R
AR
Department of Herd Health and Reproduction, Faculty of Veterinary Medicine, University of Utrecht, The Netherlands.
Steenweg
W
W
Bevers
M M
MM
de Loos
F A
FA
van den Broek
J
J
Colenbrander
B
B
D000818:Animals; D002417:Cattle / Q000502:physiology*; D005260:Female; D005298:Fertility / Q000502:physiology*; D066298:In Vitro Techniques; D008297:Male; D009865:Oocytes / Q000502:physiology; D012661:Semen / Q000166:cytology; D013084:Sperm-Ovum Interactions / Q000502:physiology*; D015044:Zona Pellucida / Q000502:physiology*