title: Cloning and sequencing of baboon and cynomolgus monkey intra-acrosomal protein SP-10: homology with human SP-10 and a mouse sperm antigen (MSA-63).
abstract: In this study, cDNAs encoding the intraacrosomal protein SP-10 were cloned and sequenced from baboon (Papio papio) and macaque (Macaca fascicularis) testis libraries and the sequence compared to that of human SP-10. Two alternatively spliced SP-10 cDNAs were obtained from both baboon and macaque testis libraries. The two cDNAs in each species contained open reading frames encoding proteins of exactly 285 and 251 amino acids. A 98% homology between baboon and macaque SP-10 was found at the protein and DNA levels. An 85% and 89% homology between baboon and macaque SP-10 and human SP-10 was present at the protein and DNA level, respectively. A mouse intraacrosomal protein, MSA-63, considered to be an SP-10 homologue, exhibited an overall 53% homology to nonhuman primate SP-10 and a 60% homology to human SP-10 at the protein level. Polymerase chain reaction analysis of testis mRNA confirmed the existence of two alternatively spliced SP-10 mRNAs in both nonhuman primates. Primer extension analysis indicated a common major transcriptional start site in baboon, macaque, and human SP-10 67 nucleotides 5' to the ATG codon. The amino acid sequence data for nonhuman primate SP-10s suggest that antibodies generated by vaccinating baboons and macaques with human SP-10 will likely recognize nonhuman primate SP-10, supporting the testing of an SP-10 contraceptive vaccine based on human SP-10 in these nonhuman primate models.
authors.affiliation: Department of Anatomy and Cell Biology, University of Virginia, Charlottesville 22908.
authors.forename: A J
authors.initials: AJ
authors.lastname: Freemerman
authors.forename: R M
authors.initials: RM
authors.lastname: Wright
authors.forename: C J
authors.initials: CJ
authors.lastname: Flickinger
authors.forename: J C
authors.initials: JC
authors.lastname: Herr
mesh_terms: D000177:Acrosome; D000595:Amino Acid Sequence; D000818:Animals; D000941:Antigens / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D003269:Contraception, Immunologic; D004247:DNA / Q000235:genetics; D012739:Gonadal Steroid Hormones / Q000235:genetics*; D006801:Humans; D008252:Macaca fascicularis / Q000235:genetics*; D008565:Membrane Proteins; D051379:Mice / Q000235:genetics*; D008969:Molecular Sequence Data; D010215:Papio / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011506:Proteins / Q000235:genetics*; D012326:RNA Splicing; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
chemical_list: C512729:ACRV1 protein, human; C080034:Acrv1 protein, mouse; D000941:Antigens; D012739:Gonadal Steroid Hormones; D008565:Membrane Proteins; D011506:Proteins; D004247:DNA