Yeasts have a four-fold variation in ribosomal DNA copy number.
By employing pulsed-field gel electrophoresis we have determined the size of the rDNA cluster in wild-type yeast strains representing genera of Candida, Kluyveromyces, Pachysolen, Schizosaccharomyces and Torulaspora. Although the genome size of the examined species is similar (12.3-13.9 Mb), at least a four-fold variation has been observed between the lowest amount of rDNA repeats in P. tannophilus (28) and the highest in C. glabrata and S. poombe (> 115). In two species the rDNA cluster is represented by two loci, residing either in one (S. pombe) or two chromosomes (C. glabrata).
Maleszka
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Molecular & Population Genetics Group, Research School of Biological Sciences, Australian National University, Canberra.
Clark-Walker
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GD
D002175:Candida / Q000235:genetics; D015342:DNA Probes; D004271:DNA, Fungal / Q000235:genetics*; D004275:DNA, Ribosomal / Q000235:genetics*; D016521:Electrophoresis, Gel, Pulsed-Field; D016681:Genome, Fungal; D007716:Kluyveromyces / Q000235:genetics; D012568:Schizosaccharomyces / Q000235:genetics; D013045:Species Specificity; D015003:Yeasts / Q000235:genetics*
D015342:DNA Probes; D004271:DNA, Fungal; D004275:DNA, Ribosomal