title: Uterine cellular changes in 2',5'-oligoadenylate synthetase during the bovine estrous cycle and early pregnancy.
abstract: The signal for maternal recognition of pregnancy in cattle is bovine trophoblast protein-1 (bTP-1), a Type I trophoblast interferon. One of the many functions of interferons is the induction of the 2',5'-oligoadenylate (2-5[A]) system, which is involved in cell division and selective degradation of mRNA. The present study focused on the cellular changes of 2-5(A) synthetase in bovine endometrium during the estrous cycle and early pregnancy. Ability of recombinant bTP-1 to stimulate activity of the enzyme in uterine cells throughout the estrous cycle was also evaluated in vitro. Surface epithelium, glandular epithelium, and stroma were enzymatically separated in cows on Day 5, 10, 15, or 18 postestrus and on Day 15 or 18 of pregnancy. Cell samples were lysed and frozen for determination of the endogenous cellular content of 2-5(A) synthetase. Additional cells obtained during the estrous cycle were cultured and treated with increasing doses of recombinant bovine interferon alpha (rbIFN-alpha) or recombinant bTP-1 (rbTP-1). During the estrous cycle, 2-5(A) synthetase was greatest on Day 5 and declined approximately 10-fold by Day 15 (p < 0.01). Cellular content of 2-5(A) synthetase was similar among all three endometrial cell types. In the gravid horn of pregnant animals, presence of a conceptus significantly increased (p < 0.01) 2-5(A) synthetase in all endometrial cell types compared to levels on Days 15 and 18 of the estrous cycle. On Day 18, levels of 2-5(A) synthetase were 30-fold greater (p < 0.01) in epithelium (surface and glandular) from pregnant cows compared to that from cyclic cows.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Animal Science, Oklahoma State University, Stillwater 74078.
authors.forename: R A
authors.initials: RA
authors.lastname: Schmitt
authors.forename: R D
authors.initials: RD
authors.lastname: Geisert
authors.forename: M T
authors.initials: MT
authors.lastname: Zavy
authors.forename: E C
authors.initials: EC
authors.lastname: Short
authors.forename: R M
authors.initials: RM
authors.lastname: Blair
mesh_terms: D015088:2',5'-Oligoadenylate Synthetase / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D004717:Endometrium / Q000166:cytology / Q000201:enzymology*; D004971:Estrus / Q000502:physiology*; D005260:Female; D007370:Interferon Type I / Q000494:pharmacology; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000494:pharmacology; D011270:Pregnancy, Animal / Q000378:metabolism*; D011994:Recombinant Proteins / Q000494:pharmacology
chemical_list: D007370:Interferon Type I; D011257:Pregnancy Proteins; D011994:Recombinant Proteins; C020947:interferon tau; D015088:2',5'-Oligoadenylate Synthetase