Distribution of TaqI sites along human chromosomes revealed by in situ enzyme-nick translation.
We have studied the relative richness of TaqI sites along human chromosomes by means of a nonradioactive in situ enzyme-nick translation procedure. Regions with a higher content of these sequences are shown to be the noncentromeric heterochromatin blocks, whereas within euchromatin, terminal R-bands are the domains more enriched in these sites. Results obtained suggest that the method of performing enzyme digestions using time as a variable, and then in situ nick translation, provides much more complete information about the distribution of enzyme sequences along chromosomes than standard enzyme digestions.
Tagarro
I
I
Departamento de Biología, Unidad de Genética, Facultad de Ciencias, Universidad Autónoma de Madrid, Spain.
Gonzalez-Aguilera
J J
JJ
Fernandez-Peralta
A M
AM
de Stefano
G F
GF
Ferrucci
L
L
D001665:Binding Sites; D002871:Chromosome Banding / Q000379:methods*; D002877:Chromosomes, Human; D015252:Deoxyribonucleases, Type II Site-Specific; D006801:Humans
D015252:Deoxyribonucleases, Type II Site-Specific; C027046:TCGA-specific type II deoxyribonucleases