Responses of neurons in and near the thalamic ventrobasal complex of the rat to stimulation of uterus, cervix, vagina, colon, and skin.
1. Previous studies in the rat and other species have shown that neurons in and near the ventrobasal complex (VB) can be activated by various visceral as well as somatic stimuli. 2. This study examined the responses of 84 single neurons in and near the rostral 2/3 of VB in 19 adult female rats in estrus to mechanical stimulation of the skin (brush, pressure, noxious pinch) and 4 different visceral stimuli, as follows: distension of both uterine horns, mechanical probing of the vagina, gentle pressure against the cervix, and distension of the colon. The rats were studied while under moderate gaseous anesthesia (33% O2-67% N2O + 0.5% halothane) and paralyzed (pancuronium bromide). 3. Of 77 neurons tested with both somatic and visceral stimuli, 70 were responsive to one type and/or the other. Responses to somatic stimuli were immediate with brief afterdischarges to the pinch stimuli. In contrast, responses to visceral stimuli were delayed an average of 9 s with long afterdischarges averaging 2 min. Most viscerally responsive neurons (74%) had somatic receptive fields, often (44%) to noxious pinch. 4. Of the 70 responsive neurons, 43 (61%) responded to 1 or more of the 4 visceral stimuli, primarily with excitation. Most of these 43 neurons (71%) were responsive to uterine distension, whereas fewer responded to stimulation of the cervix (45%), vagina (29%), or colon (34%). 5. Viscerally responsive neurons were preferentially located in regions bordering or near VB. Only 6 of 22 neurons within the core of VB (27%) responded to visceral stimuli, in contrast with 37 of 48 neurons bordering or near VB (77%). 6. The six viscerally responsive neurons within VB all had somatic receptive fields located primarily on the caudal part of the body and were responsive to only one or two of the four visceral stimuli, usually the uterus. The 37 viscerally responsive neurons bordering or near VB were of 3 types. Neurons of the first type (n = 15) were scattered throughout the areas bordering VB and responded to both somatic and visceral stimuli much like VB neurons, except that they showed more visceral convergence. Neurons of the second type (n = 11) were concentrated at the rostral and dorsal borders of VB and responded only to visceral stimuli, mainly the uterus. Neurons of the third type (n = 11) were concentrated ventrally and had very complex, long-lasting and history-dependent response characteristics to both visceral and somatic stimuli.(ABSTRACT TRUNCATED AT 400 WORDS)
Berkley
K J
KJ
Institute National de la Santé et de la Recherche Médicale U. 161, Laboratoire de Physiopharmacologie de la Douleur, Ecole Pratique des Hautes Etudes, Paris, France.
Guilbaud
G
G
Benoist
J M
JM
Gautron
M
M
D000818:Animals; D002584:Cervix Uteri / Q000294:innervation / Q000502:physiology*; D003106:Colon / Q000294:innervation / Q000502:physiology*; D004558:Electric Stimulation; D004566:Electrodes; D004569:Electroencephalography; D004971:Estrus / Q000502:physiology; D005260:Female; D009474:Neurons / Q000502:physiology*; D010146:Pain / Q000503:physiopathology; D010812:Physical Stimulation; D051381:Rats; D012867:Skin / Q000294:innervation; D012879:Skin Physiological Phenomena; D013238:Stereotaxic Techniques; D013787:Thalamic Nuclei / Q000033:anatomy & histology / Q000166:cytology / Q000502:physiology*; D014599:Uterus / Q000294:innervation / Q000502:physiology*; D014621:Vagina / Q000294:innervation / Q000502:physiology*