Participation of the overproduced elongation factor Tu from Thermus thermophilus in protein biosynthesis of Escherichia coli.
The influence of the overproduced elongation factor Tu (EF-Tu) from Thermus thermophilus on the protein biosynthesis in Escherichia coli was investigated both in vivo and in vitro. A kirromycin-resistant E. coli strain became sensitive to this antibiotic upon the expression of the tuf A-gene of T. thermophilus present on a plasmid. In in vitro translation with components of the kirromycin-resistant E. coli strain the poly(Phe) synthesis stopped when minute amounts of the EF-Tu from T. thermophilus were added. Both results indicate the sensitivity of the T. thermophilus EF-Tu to kirromycin and its participation in the polypeptide synthesis of E. coli.
Zeidler
W
W
Laboratorium für Biochemie, Universität Bayreuth, Germany.
Kreutzer
R
R
Sprinzl
M
M
D001426:Bacterial Proteins / Q000096:biosynthesis*; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D010452:Peptide Biosynthesis; D010444:Peptide Elongation Factor Tu / Q000235:genetics / Q000378:metabolism*; D010455:Peptides; D010957:Plasmids; D014176:Protein Biosynthesis; D011728:Pyridones / Q000494:pharmacology; D012270:Ribosomes / Q000378:metabolism; D016963:Thermus thermophilus / Q000737:chemistry* / Q000235:genetics; D014169:Transformation, Bacterial
D001426:Bacterial Proteins; D010455:Peptides; D011728:Pyridones; C012001:polyphenylalanine; D010444:Peptide Elongation Factor Tu; C008536:mocimycin