A new luciferase promoter insertion vector for the analysis of weak transcriptional activities.
A new luciferase-encoding expression vector was generated by inserting the strong transcription termination signal from the mouse c-mos oncogene upstream from a multiple cloning site. This construct significantly reduced background transcription in NIH3T3 cells and has proven useful in the study of a weak promoter from the murine growth-arrest-specific gene gas-1.
de Martin
R
R
European Molecular Biology Laboratory, Heidelberg, Germany.
Strasswimmer
J
J
Philipson
L
L
D016475:3T3 Cells; D000818:Animals; D002455:Cell Division / Q000235:genetics*; D016258:Genes, mos; D005822:Genetic Vectors; D008156:Luciferases / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D010957:Plasmids; D011401:Promoter Regions, Genetic; D015183:Restriction Mapping; D013728:Terminator Regions, Genetic; D014158:Transcription, Genetic; D014162:Transfection / Q000379:methods*
D008156:Luciferases