Optimization of microsatellite analysis for genetic mapping.
A method for typing microsatellite polymorphisms is described. It involves amplification using the polymerase chain reaction with one primer 5' end-labeled with 32P. Alleles are separated by denaturing gel electrophoresis and detected by autoradiography. Standardized conditions allow accurate typing of almost all microsatellite polymorphisms, and results are usually obtained within 24 h.
Hughes
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AE
Department of Medical Genetics, Queen's University of Belfast, Belfast City Hospital, United Kingdom.
D001345:Autoradiography; D002874:Chromosome Mapping / Q000379:methods*; D004276:DNA, Satellite; D005260:Female; D006801:Humans; D008297:Male; D010375:Pedigree; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic
D004276:DNA, Satellite