title: Frequent c-Ki-ras oncogene activation in mucous cell hyperplasias of pancreas suffering from chronic inflammation.
abstract: In order to scrutinize the possible significance of (nonatypical) mucous cell hyperplasia of the pancreas to neoplasia, we analyzed these lesions in terms of c-Ki-ras activation, which is known to be very frequent in pancreatic carcinomas. A total of 16 such mucous cell hyperplasias were collected from 10 pancreases resected for chronic pancreatitis. Tiny tissue fragments were taken from hematoxylin-stained sections by microdissection, and DNA analysis was carried out by the polymerase chain reaction amplification and oligonucleotide hybridization methods. Activating mutations of c-Ki-ras oncogene at codon 12 were detected in 10 of the 16 lesions (62.5%), a high rate as seen in carcinomas. The results indicated a clonal origin of cells comprising the mucous cell hyperplasia suggesting a neoplastic and/or precancerous nature.
authors.affiliation: Department of Pathology, Cancer Institute, Tokyo, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Yanagisawa
authors.forename: K
authors.initials: K
authors.lastname: Ohtake
authors.forename: K
authors.initials: K
authors.lastname: Ohashi
authors.forename: M
authors.initials: M
authors.lastname: Hori
authors.forename: T
authors.initials: T
authors.lastname: Kitagawa
authors.forename: H
authors.initials: H
authors.lastname: Sugano
authors.forename: Y
authors.initials: Y
authors.lastname: Kato
mesh_terms: D000328:Adult; D000368:Aged; D001483:Base Sequence; D002908:Chronic Disease; D005260:Female; D005786:Gene Expression Regulation; D011905:Genes, ras; D006801:Humans; D006965:Hyperplasia; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D010179:Pancreas / Q000378:metabolism / Q000473:pathology*; D010195:Pancreatitis / Q000235:genetics* / Q000473:pathology; D017354:Point Mutation