title: Identification of a Saccharomyces cerevisiae mutation that allows cells to grow without chitin synthase 1 or 2.
abstract: Chitin is a component of the yeast cell wall which is localized to the septum between mother and daughter cells. Previous work in Saccharomyces cerevisiae has shown that this organism possesses three chitin synthases, 1, 2, and 3. Disruption experiments have shown that loss of chitin synthase 2 has a more profound effect on cell viability than loss of either of the other two and is lethal in complete media. We report here the finding of an S. cerevisiae strain which does not require the chitin synthase 2 structural gene for viability. We present evidence that there is a gene in this strain which suppresses the lethality of disruption of the chitin synthase 2 structural gene and is genetically distinct from the structural genes for chitin synthase 1 and chitin synthase 2. We show that an S. cerevisiae mutant containing the suppressor and lacking both structural genes for chitin synthase 1 and 2 has normal amounts of chitin in its cell wall. We hypothesize that the suppressor gene encodes or controls the expression of chitin synthase 3.
authors.affiliation: Department of Microbial Genetics and Biochemistry, Bristol Meyers Squibb Institute for Pharmaceutical Research, Princeton, NJ 08543.
authors.forename: J
authors.initials: J
authors.lastname: Baymiller
authors.forename: J E
authors.initials: JE
authors.lastname: McCullough
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology; D002687:Chitin Synthase / Q000235:genetics* / Q000378:metabolism; D005800:Genes, Fungal; D005808:Genes, Recessive; D016153:Genes, Suppressor; D007527:Isoenzymes / Q000235:genetics* / Q000378:metabolism; D009154:Mutation; D010455:Peptides / Q000494:pharmacology; D010641:Phenotype; D011741:Pyrimidine Nucleosides / Q000494:pharmacology; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development
chemical_list: D000935:Antifungal Agents; D007527:Isoenzymes; D010455:Peptides; D011741:Pyrimidine Nucleosides; C002457:polyoxin; D002687:Chitin Synthase