Identification and quantitation of intact diastereoisomeric benzodiazepine glucuronides in biological samples by high-performance liquid chromatography.
A rapid and simple high-performance liquid chromatographic method for the simultaneous detection of intact glucuronides of different benzodiazepines is described. Separation of the diastereomers of the following benzodiazepine glucuronides can be achieved on a reversed-phase column (octadecyl or select B): oxazepam, nordiazepam, temazepam, lorazepam and 3-hydroxyprazepam. If the sample contains both S-lorazepam and R-temazepam glucuronides, or both S-temazepam and nordiazepam glucuronides, further separation on a beta-cyclodextrin column is required. The detection limit ranges between 5 and 10 ng of glucuronides per ml of plasma or urine, respectively.
Franzelius
C
C
Institute of Forensic Medicine, University of Tübingen, Germany.
Besserer
K
K
D001569:Benzodiazepines / Q000032:analysis* / Q000097:blood / Q000652:urine; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005965:Glucuronates / Q000032:analysis* / Q000097:blood / Q000652:urine; D006801:Humans; D013057:Spectrum Analysis; D013237:Stereoisomerism
D005965:Glucuronates; D001569:Benzodiazepines