title: Nucleotide sequence of a cDNA for canine beta-spectrin reveals high evolutionary conservation.
abstract: A cDNA coding for the carboxy-terminus of canine beta-spectrin was isolated from a canine tracheal cDNA library. Analysis of the 3267 nucleotide sequence revealed a single open reading frame coding for 707 amino acids. Comparison of the deduced amino acid sequence to that of the recently reported general isoform of human beta-spectrin (beta G) revealed 98% identity. This high degree of conservation of the general isoform of beta-spectrin illustrates a strong evolutionary selection and should help in identification of sites that are candidates to mediate specialized functions of this general isoform. This is the first report of a cDNA encoding canine beta-spectrin.
authors.affiliation: College of Pharmacy, University of Oklahoma Health Sciences Center, Oklahoma City 73190.
authors.forename: S
authors.initials: S
authors.lastname: Tan
authors.forename: V
authors.initials: V
authors.lastname: Shankar
authors.forename: M S
authors.initials: MS
authors.lastname: Gilmore
authors.forename: G P
authors.initials: GP
authors.lastname: Sachdev
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005075:Biological Evolution; D004247:DNA / Q000235:genetics*; D004285:Dogs; D006801:Humans; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D012091:Repetitive Sequences, Nucleic Acid; D017386:Sequence Homology, Amino Acid; D013049:Spectrin / Q000235:genetics*; D014132:Trachea / Q000502:physiology
chemical_list: D013049:Spectrin; D004247:DNA