Selective removal of beta 2-microglobulin from plasma specimens of long-term hemodialysis patients by high-performance immunoaffinity chromatography.
We examined the suitability of high-performance immunoaffinity chromatography (HPIAC) for removing beta 2-microglobulin (beta 2-MG) from plasma of patients (n = 26) undergoing long-term hemodialysis. We used immobilized antibody to beta 2-MG to prepare an immunoaffinity column with a 0.5-mL bed volume. Plasma samples of 0.5 mL were chromatographed at a flow rate of 0.5 mL/min and pressures < 7 kg/cm2. Although only a small amount of beta 2-MG can be removed with a conventional dialyzer, 92.1% +/- 0.4% of the globulin could be removed by HPIAC. The column could be reused after acid elution. After 50 cycles the efficiency of beta 2-MG trapping was 73% of the original efficiency.
Mogi
M
M
Department of Oral Biochemistry, Matsumoto Dental College, Shiojiri, Japan.
Harada
M
M
Kojima
K
K
Adachi
T
T
Nakamura
S
S
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015153:Blotting, Western; D002846:Chromatography, Affinity / Q000379:methods*; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006801:Humans; D007074:Immunoglobulin G; D007158:Immunologic Techniques; D008297:Male; D008875:Middle Aged; D006435:Renal Dialysis; D001613:beta 2-Microglobulin / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification*
D007074:Immunoglobulin G; D001613:beta 2-Microglobulin