Identity of the major cysteine proteinase (cruzipain) from Trypanosoma cruzi and an antigen (Ag163B6) isolated with a monoclonal antibody.
Cruzipain, purified by conventional methods, and Ag163B6, isolated by affinity chromatography with a monoclonal antibody raised against a T. cruzi extract, are glycoproteins with a similar electrophoretic mobility, which reacted with sera from most chronic chagasic patients. Their behaviour in SDS-PAGE, Western blotting, isoelectric focusing, two-dimensional electrophoresis (IEF and SDS-PAGE), Ouchterlony's double diffusion, and enzyme activity in SDS-PAGE gels containing 0.1% gelatin suggests that they are identical.
Malchiodi
E L
EL
Instituto de Estudios de la Immunidad Humoral (CONICET/UBA), Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Argentina.
Chiaramonte
M G
MG
Martínez
J A
JA
Zwirner
N W
NW
Margni
R A
RA
Cazzulo
J J
JJ
D000818:Animals; D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan / Q000737:chemistry / Q000302:isolation & purification*; D015153:Blotting, Western; D003546:Cysteine Endopeptidases / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification; D015180:Electrophoresis, Gel, Two-Dimensional; D005779:Immunodiffusion; D007525:Isoelectric Focusing; D015800:Protozoan Proteins; D014349:Trypanosoma cruzi / Q000201:enzymology* / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan; D015800:Protozoan Proteins; D003546:Cysteine Endopeptidases; C063214:cruzipain