Sensitive high-performance liquid chromatographic assay for the determination of chlorhexidine in saliva.
A high-performance liquid chromatographic assay was developed to determine salivary chlorhexidine concentration. Saliva sample (200 microliters) was extracted into methylene chloride. Chromatographic separation was achieved with a C18 column using a mobile phase of acetonitrile-0.05 M sodium acetate and 0.005 M heptanesulfonic acid (40:60, v/v). The standard curve was linear from 0.051 to 20.48 micrograms/ml (r2 > or = 0.997). Intra-day and inter-day coefficients of variation were < 5.5 and < or = 9%, respectively. The assay is rapid, sensitive, simple, and successfully used for quantitating salivary chlorhexidine content released from a chlorhexidine-impregnated resin worn by patients.
Lam
Y W
YW
Department of Pharmacology, University of Texas Health Science Center, San Antonio 78284.
Chan
D C
DC
Rodriguez
S Y
SY
Lintakoon
J H
JH
Lam
T H
TH
D002710:Chlorhexidine / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007202:Indicators and Reagents; D009967:Orthodontic Appliances; D012463:Saliva / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
D007202:Indicators and Reagents; D002710:Chlorhexidine