Determination of leucovorin and 5-fluorouracil in plasma by high-performance liquid chromatography.
A method was developed for the determination of (6R)- and (6S)-leucovorin and 5-fluorouracil in plasma. As leucovorin diastereoisomers cannot be separated on a classical reversed-phase column, it was necessary to use a chiral stationary phase. The method presented is based on the same principle as the method described by Wainer and Stiffin [J. Chromatogr., 424 (1988) 158], i.e., coupling of a bovine serum albumin phase to an achiral stationary phase. Before the chromatography, the drug was isolated from the plasma matrix by solid-phase extraction. For 5-fluorouracil, chromatography was performed on a classical RP-18 column after extraction from the plasma by liquid-liquid extraction. Both methods were validated and applied to the analysis of patients' samples.
Vandenbosch
C
C
Department of Pharmaceutical and Biomedical Analysis, Vrije Universiteit Brussel, Belgium.
van Belle
S
S
de Smet
M
M
Taton
G
G
Bruynseels
V
V
Vandenhoven
G
G
Massart
D L
DL
D002021:Buffers; D002851:Chromatography, High Pressure Liquid; D005472:Fluorouracil / Q000097:blood* / Q000493:pharmacokinetics; D006801:Humans; D007275:Injections, Intravenous; D002955:Leucovorin / Q000097:blood* / Q000493:pharmacokinetics; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism
D002021:Buffers; D002955:Leucovorin; D005472:Fluorouracil