title: Chronic catheterization of external iliac vessels in growing cattle.
abstract: Bovine preparations that allow for in vivo measurement of metabolic fluxes across the hindlimb often suffer from limited durability, usually because of failure of the venous catheter. A catheterization procedure that virtually eliminates this occurrence is presented. A silicone rubber catheter is implanted permanently in the femoral vein. It accommodates the repeated insertion and removal at each sampling session of a temporary sampling catheter. A simple and reliable method ensures the positioning of this catheter in the external iliac vein of a conscious, normally standing animal. The application of this approach allowed the study of hindlimb metabolism of cattle for up to 4 mo without a single planned sampling session postponed or missed. This preparation is particularly well suited for studies that require repeated measurements of hindlimb metabolism on the same animals over a period of many months.
authors.affiliation: Department of Animal Science, Cornell University, Ithaca, New York 14853.
authors.forename: Y R
authors.initials: YR
authors.lastname: Boisclair
authors.forename: A W
authors.initials: AW
authors.lastname: Bell
authors.forename: D E
authors.initials: DE
authors.lastname: Bauman
mesh_terms: D000818:Animals; D001800:Blood Specimen Collection; D002406:Catheterization, Peripheral; D002408:Catheters, Indwelling; D002417:Cattle; D005268:Femoral Vein / Q000502:physiology; D006614:Hindlimb / Q000098:blood supply / Q000502:physiology; D007084:Iliac Vein / Q000502:physiology*; D008297:Male; D012039:Regional Blood Flow / Q000502:physiology; D012501:Saphenous Vein / Q000502:physiology