Cloning and sequencing of a full length cDNA encoding ovine lipoprotein lipase.
A cDNA clone encoding lipoprotein lipase has been isolated from an ovine adipocyte library. Sequencing of this clone has revealed a single open reading frame encoding a 450 amino acid protein. Comparison with known LPL sequences from other species shows a high degree of conservation in the sequence of the protein and in the 5' untranslated region of the DNA sequence.
Edwards
W D
WD
Department of Biochemistry, University of Wales, Cardiff, UK.
Daniels
S E
SE
Page
R A
RA
Volpe
C P
CP
Kille
P
P
Sweeney
G E
GE
Cryer
A
A
D000273:Adipose Tissue / Q000201:enzymology*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods; D004247:DNA / Q000235:genetics*; D015723:Gene Library; D006801:Humans; D008071:Lipoprotein Lipase / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D012756:Sheep
D004247:DNA; D008071:Lipoprotein Lipase