title: Clinical detection of BCR-abl fusion by in situ hybridization in chronic myelogenous leukemia.
abstract: We describe the use of the fluorescence in situ hybridization (FISH) technique to detect residual Philadelphia chromosome-positive (Ph+) cells in a patient with blastic phase chronic myelogenous leukemia (CML) after aggressive cytoreductive treatment. The analysis was made in interphase nuclei because of the very small number of recognizable metaphases in leukemic patients. FISH was a reliable tool for the detection of chromosome translocations in interphase nuclei as compared with conventional cytogenetic and polymerase chain reaction (PCR) techniques.
authors.affiliation: Genetics Unit, Meir General Hospital, Kfar-Saba, Israel.
authors.forename: A
authors.initials: A
authors.lastname: Amiel
authors.forename: S
authors.initials: S
authors.lastname: Yarkoni
authors.forename: M
authors.initials: M
authors.lastname: Fejgin
authors.forename: E
authors.initials: E
authors.lastname: Gaber
authors.forename: A
authors.initials: A
authors.lastname: Nagler
authors.forename: Y
authors.initials: Y
authors.lastname: Manor
authors.forename: M
authors.initials: M
authors.lastname: Lishner
mesh_terms: D000328:Adult; D002892:Chromosomes, Human, Pair 22; D002899:Chromosomes, Human, Pair 9; D016313:Genes, abl; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007399:Interphase; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000188:drug therapy / Q000235:genetics*; D008297:Male; D010677:Philadelphia Chromosome; D016133:Polymerase Chain Reaction; D012074:Remission Induction; D014178:Translocation, Genetic