Physical linkage of the SLG and SRK genes at the self-incompatibility locus of Brassica oleracea.
In Brassica oleracea, the pollen-stigma interaction of self-incompatibility is controlled by a single genetically defined locus designated S. Molecular studies have identified two genes that are tightly linked to the classically defined S locus: The S-Locus Glycoprotein (SLG) gene and the S-Receptor Kinase (SRK) gene. In previous RFLP linkage analyses with probes specific for SLG and SRK, we were unable to identify any recombination events between SLG, SRK, and self-incompatibility phenotype. In this paper, we use pulsed-field gel electrophoresis (PFGE) in conjunction with DNA blot analysis to characterize the S-locus region from two highly divergent self-incompatibility genotypes, S2 and S6. We establish the physical linkage of SLG and SRK in each genotype, and demonstrate that the two genes are separated by a maximum distance of 220 kb in the S6 genotype and 350 kb in the S2 genotype. Furthermore, a comparison of the data from the two genotypes reveals that a high level of polymorphism exists across the entire S-locus region.
Boyes
D C
DC
Division of Biological Sciences, Cornell University, Ithaca, New York 14853.
Nasrallah
J B
JB
D001937:Brassica / Q000235:genetics*; D003433:Crosses, Genetic; D015342:DNA Probes; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D016521:Electrophoresis, Gel, Pulsed-Field; D017343:Genes, Plant / Q000235:genetics*; D008040:Genetic Linkage; D006023:Glycoproteins / Q000235:genetics; D006239:Haplotypes; D006720:Homozygote; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010940:Plant Proteins / Q000235:genetics; D011058:Pollen / Q000502:physiology; D011494:Protein Kinases / Q000235:genetics
D015342:DNA Probes; D006023:Glycoproteins; D010940:Plant Proteins; C064156:self-incompatibility locus specific glycoprotein, Brassica; D011494:Protein Kinases; C079832:S-receptor kinase; D015252:Deoxyribonucleases, Type II Site-Specific