title: An enhancer unit of L-type pyruvate kinase gene is responsible for transcriptional stimulation by dietary fructose as well as glucose in transgenic mice.
abstract: We produced three lines of transgenic mice containing the 5' flanking region of the L-type pyruvate kinase gene from nucleotides -189 to +37, which includes an enhancer unit and TATA box as functional elements, linked to the chloramphenicol acetyltransferase gene. Since transgene expression was stimulated by both dietary fructose and glucose in a tissue-dependent manner, we suggest that this unit is responsive to both stimuli.
authors.affiliation: Department of Nutrition and Physiological Chemistry, Osaka University Medical School, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Noguchi
authors.forename: M
authors.initials: M
authors.lastname: Okabe
authors.forename: Z
authors.initials: Z
authors.lastname: Wang
authors.forename: K
authors.initials: K
authors.lastname: Yamada
authors.forename: E
authors.initials: E
authors.lastname: Imai
authors.forename: T
authors.initials: T
authors.lastname: Tanaka
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D004032:Diet; D004742:Enhancer Elements, Genetic; D005632:Fructose / Q000008:administration & dosage / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects; D005947:Glucose / Q000494:pharmacology*; D051379:Mice; D008822:Mice, Transgenic; D011770:Pyruvate Kinase / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D012045:Regulatory Sequences, Nucleic Acid; D016385:TATA Box; D014158:Transcription, Genetic / Q000187:drug effects*
chemical_list: D012333:RNA, Messenger; D005632:Fructose; D015500:Chloramphenicol O-Acetyltransferase; D011770:Pyruvate Kinase; D005947:Glucose