Autoantibodies directed against different classes of Fc gamma R are found in sera of autoimmune patients.
Serum samples from 147 patients with different systemic autoimmune diseases (SLE, Sjögren's syndrome, and progressive systemic sclerosis) were tested for anti-Fc gamma R activity using mouse rFc gamma RII in an ELISA. High reactivity compared to normal individuals was found for patients with all three diseases. The anti-Fc gamma R antibody was purified from several serum samples by affinity chromatography on a Sepharose column coupled with denatured, murine rFc gamma RII. Both IgM and IgG antibodies were found. To analyze the specificity of the affinity-purified autoantibody, cells (human neutrophils, IFN-gamma-stimulated neutrophils, monocytes, and the THP-1 monocytic cell line) that express different combinations of Fc gamma R (CD64, CD32, CD16) were stained with the affinity purified Ig. Ig directed against all three types of Fc gamma R were found. The results may reflect on the role of Fc gamma R-specific antibodies in the pathology of autoimmune diseases.
Boros
P
P
Department of Biochemistry, Mount Sinai School of Medicine, New York, NY 10029.
Muryoi
T
T
Spiera
H
H
Bona
C
C
Unkeless
J C
JC
D000818:Animals; D001323:Autoantibodies / Q000097:blood* / Q000276:immunology / Q000302:isolation & purification; D001327:Autoimmune Diseases / Q000276:immunology*; D002846:Chromatography, Affinity; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000276:immunology; D051379:Mice; D017452:Receptors, IgG / Q000276:immunology*; D012595:Scleroderma, Systemic / Q000276:immunology; D012859:Sjogren's Syndrome / Q000276:immunology
D001323:Autoantibodies; D017452:Receptors, IgG