Factors affecting fertility in the postpartum cow: role of the oocyte and follicle in conception rate.
Four experiments were designed to examine the contribution of the oocyte or the follicular, oviductal, or early uterine environments to low fertility associated with the first ovulation postpartum. At 17-25 days postpartum in experiments 1, 2, and 3, suckled beef cows were assigned at random to receive 6 mg norgestomet, via ear-implant, for 9 days (NOR) or to serve as controls (CON). Calves were weaned from all cows 7 days after assignment to treatment in order to induce estrus, an LH surge, ovulation, and subsequent formation of CL. As cows were detected to be in estrus, they were bred first by natural service and 12 h later by artificial insemination. In experiment 1, on Day 3 after estrus, the oviduct ipsilateral to the side of ovulation was removed and flushed for recovery of an embryo or oocyte. Rates of recovery (86%), fertilization (68%), and development of fertilized oocytes to the 4- to 8-cell stage (100%) did not differ between CON and NOR cows. In experiment 2, uteri were flushed nonsurgically on Day 6 after estrus. Rates of recovery of embryos from the uterus were similar between CON (86%) and NOR (71%) cows. In experiment 3, one half of the cows in each group (CON and NOR) were fed melengestrol acetate (MGA) beginning on Day 4 after estrus and continuing until Day 35. The remaining cows in each group served as controls. Treatment with NOR increased (p < 0.05) the proportion of cows that maintained pregnancy until Day 35 (9/22) as compared to controls (0/18).(ABSTRACT TRUNCATED AT 250 WORDS)
Breuel
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Division of Animal and Veterinary Science, West Virginia University, Morgantown 26506.
Lewis
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Schrick
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Lishman
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Inskeep
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Butcher
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D000818:Animals; D001755:Blastocyst / Q000166:cytology / Q000187:drug effects; D002417:Cattle; D005260:Female; D005298:Fertility / Q000187:drug effects / Q000502:physiology*; D008183:Luteal Phase / Q000187:drug effects / Q000502:physiology; D008552:Melengestrol Acetate / Q000494:pharmacology; D009865:Oocytes / Q000254:growth & development / Q000502:physiology*; D006080:Ovarian Follicle / Q000502:physiology*; D049590:Postpartum Period / Q000502:physiology*; D011247:Pregnancy; D011255:Pregnancy Maintenance / Q000187:drug effects / Q000502:physiology; D011282:Pregnenediones / Q000494:pharmacology; D045167:Progesterone Congeners / Q000494:pharmacology
D011282:Pregnenediones; D045167:Progesterone Congeners; C100284:norgestomet; D008552:Melengestrol Acetate