The identification of Y chromosome-linked markers with random sequence oligonucleotide primers.
The polymerase chain reaction (PCR)-based technique of random amplification of polymorphic DNA (RAPD) is extremely useful for developing DNA-based markers. We previously identified a linkage group of eight unmapped RAPD markers that distinguish C57BL/6J and DBA/2J mice (Mammalian Genome 3: Woodward et al., 73-78, 1992). In this study, we report that all eight markers are Y Chromosome (Chr)-linked. One additional Y-linked RAPD was discovered serendipitously during the screening of a C3H/HeJ x (C3H/HeJ x SJL/J)F1 BC1 population. The segregation of all nine markers was analyzed with a panel of 14 independent inbred strains of male mice. The nine markers could be divided into three distinct groups: (1) DYByu2, DYByu5, DYByu6, and DYByu8 identify both the M.m. musculus and M.m. domesticus type Y Chr; (2) DYByu1, DYByu3, DYByu4, and DYByu7 are specific for the M.m. musculus type; and (3) DYByu9 is specific for the M.m. domesticus type. The results clearly indicate that the RAPD technique can be used to identify Y Chr-linked, DNA-based markers in mammalian species.
Wardell
B B
BB
Department of Microbiology, Brigham Young University, Provo, Utah 84602.
Sudweeks
J D
JD
Meeker
N D
ND
Estes
S S
SS
Woodward
S R
SR
Teuscher
C
C
D000818:Animals; D001483:Base Sequence; D004277:DNA, Single-Stranded; D005260:Female; D008040:Genetic Linkage; D005819:Genetic Markers; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D013045:Species Specificity; D014998:Y Chromosome
D004277:DNA, Single-Stranded; D005819:Genetic Markers