title: Restricted canine distemper virus infection of oligodendrocytes.
abstract: BACKGROUND
  Canine distemper virus, a morbillivirus induces multifocal demyelination in the central nervous system. The acute demyelination correlates with virus replication in brain cells, especially astrocytes. Observations in vivo and in vitro demonstrated degeneration of oligodendrocytes, the myelin producing cells. However, the mechanism of oligodendroglial degeneration in distemper remained unexplained. Infection of the myelin producing cells, the most obvious explanation for the phenomenon of demyelination, could not be supported by extensive searches for viral particles or antigens in these cells neither in vivo nor in vitro.
  
  
  METHODS
  In the present study, we combined in situ hybridization to visualize viral nucleic acid sequences with immunofluorescence for oligodendroglial antigens.
  
  
  RESULTS
  The nonradioactive in situ hybridization technique in combination with contrast enhanced video microscopy allowed us to unequivocally demonstrate the presence of canine distemper virus nucleic acid sequences in cultured oligodendrocytes. Many oligodendrocytes close to infected foci in the brain cell cultures were found to contain viral nucleic acid sequences. Only 1% of the viral nucleic acid sequences containing oligodendrocytes also contained viral antigen. Canine distemper virus replication in these cells is clearly restricted.
  
  
  CONCLUSIONS
  Different possibilities why oligodendrocytes do not support a productive virus infection and mechanisms by which such a restricted infection leads to oligodendroglial degeneration and ensuing demyelination are discussed. While our results have advanced our understanding of the pathogenesis of acute demyelination in distemper, they may also offer a possible explanation for the chronic progressive or even relapsing course of the disease. A restricted infection of the oligodendrocytes may be the mechanism by which canine distemper virus persists in the central nervous system. Virus persistence is probably a key event in many chronic viral induced inflammatory demyelinating diseases.
authors.affiliation: Department of Animal Neurology, University of Berne, Switzerland.
authors.forename: A
authors.initials: A
authors.lastname: Zurbriggen
authors.forename: M
authors.initials: M
authors.lastname: Yamawaki
authors.forename: M
authors.initials: M
authors.lastname: Vandevelde
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D001921:Brain / Q000166:cytology / Q000382:microbiology; D002478:Cells, Cultured; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics; D003711:Demyelinating Diseases; D004216:Distemper / Q000473:pathology*; D004217:Distemper Virus, Canine / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D004285:Dogs; D005455:Fluorescent Antibody Technique; D015967:Gene Expression Regulation, Viral / Q000235:genetics; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D009836:Oligodendroglia / Q000382:microbiology* / Q000473:pathology; D012367:RNA, Viral / Q000032:analysis / Q000235:genetics
chemical_list: D000956:Antigens, Viral; D004279:DNA, Viral; D012367:RNA, Viral