title: Cholesterol increase in mitochondria: its effect on inner-membrane functions, submitochondrial localization and ultrastructural morphology.
abstract: The effect of cholesterol incorporation on some functions of the mitochondrial inner membrane and on the morphology of rat liver mitochondria was studied. Basal ATPase and succinate dehydrogenase activities remained unchanged after cholesterol was incorporated into the mitochondria; however, uncoupled ATPase activity was partially inhibited. The presence of several substrates and inhibitors did not change the amount of cholesterol incorporated, which was localized mostly in the outer membrane. Electron-microscope observations revealed the presence of vesicles between the outer and inner membranes; these vesicles increased in number with the amount of cholesterol incorporated. The data suggest that cholesterol induces the formation of vesicles from the outer membrane, and modifies the activity of stimulated ATPase.
authors.affiliation: Departamento de Bioquímica, Facultad de Medicina, Universidad Nacional Autónoma de México, D.F.
authors.forename: S
authors.initials: S
authors.lastname: Echegoyen
authors.forename: E B
authors.initials: EB
authors.lastname: Oliva
authors.forename: J
authors.initials: J
authors.lastname: Sepulveda
authors.forename: J C
authors.initials: JC
authors.lastname: Díaz-Zagoya
authors.forename: M T
authors.initials: MT
authors.lastname: Espinosa-García
authors.forename: J P
authors.initials: JP
authors.lastname: Pardo
authors.forename: F
authors.initials: F
authors.lastname: Martínez
mesh_terms: D000251:Adenosine Triphosphatases / Q000032:analysis* / Q000187:drug effects; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002784:Cholesterol / Q000378:metabolism* / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D007425:Intracellular Membranes / Q000187:drug effects / Q000201:enzymology*; D008928:Mitochondria / Q000187:drug effects / Q000201:enzymology* / Q000648:ultrastructure; D051381:Rats; D012710:Serum Albumin, Bovine / Q000494:pharmacology; D013385:Succinate Dehydrogenase / Q000032:analysis* / Q000187:drug effects; D013997:Time Factors
chemical_list: D012710:Serum Albumin, Bovine; D000255:Adenosine Triphosphate; D002784:Cholesterol; D013385:Succinate Dehydrogenase; D000251:Adenosine Triphosphatases