Analysis of sequence variation in grapevine yellow speckle viroid 1 reveals two distinct alternative structures for the pathogenic domain.
The nucleotide sequences of 24 full-length cDNA clones prepared from a field isolate of grapevine yellow speckle viroid 1 (GYSV 1) have been determined and compared with that of the previously published GYSV 1 sequence. A large number of sequence variations were observed, the majority of which occurred in the pathogenicity domain (P domain) of the viroid. The GYSV 1 variants could be divided into two types each containing a distinct secondary structure within the P domain. Monomeric exact-length RNA transcripts produced from individual clones belonging to both types were infectious in viroid-free grapevines and produced homogenous populations of progeny viroid in vivo.
Rigden
J E
JE
CSIRO Division of Horticulture, Adelaide, Australia.
Rezaian
M A
MA
D001483:Base Sequence; D003001:Cloning, Molecular; D005638:Fruit / Q000382:microbiology; D014644:Genetic Variation / Q000235:genetics*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010942:Plant Viruses / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics; D014772:Viroids / Q000235:genetics*
D012367:RNA, Viral