MK801 induces immediate-early gene proteins and BDNF mRNA in rat cerebrocortical neurones.
Recent studies have shown that MK801, a potent phencyclidine receptor ligand, causes pathomorphological changes in rat cerebrocortical neurones. Here we report that doses of MK801 (1 and 5 mg kg-1) which have been shown to produce pathomorphological changes, induce the expression of immediate-early gene proteins (IEGPs) and brain-derived neurotrophic factor (BDNF) mRNA in rat cerebrocortical neurones. Blockade of central muscarinic receptors which has been shown to prevent MK801-induced pathomorphological changes in cerebrocortical neurones, also prevented MK801-induced expression of IEGPs and BDNF mRNA. The transiently increased expression of BDNF mRNA may be an acute compensatory response of these neurones to MK801-induced injury.
Hughes
P
P
Department of Pharmacology, School of Medicine, University of Auckland, New Zealand.
Dragunow
M
M
Beilharz
E
E
Lawlor
P
P
Gluckman
P
P
D000818:Animals; D019208:Brain-Derived Neurotrophic Factor; D002540:Cerebral Cortex / Q000187:drug effects / Q000502:physiology*; D004268:DNA-Binding Proteins / Q000032:analysis / Q000235:genetics; D016291:Dizocilpine Maleate / Q000494:pharmacology*; D051766:Early Growth Response Protein 1; D016762:Genes, fos / Q000187:drug effects; D016758:Genes, jun / Q000187:drug effects; D017874:Immediate-Early Proteins; D017403:In Situ Hybridization; D008297:Male; D009414:Nerve Growth Factors / Q000235:genetics; D009419:Nerve Tissue Proteins / Q000235:genetics*; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D016760:Proto-Oncogene Proteins c-fos / Q000032:analysis / Q000235:genetics; D016755:Proto-Oncogene Proteins c-jun / Q000032:analysis / Q000235:genetics; D011519:Proto-Oncogenes / Q000187:drug effects*; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D014157:Transcription Factors / Q000032:analysis / Q000235:genetics; D016335:Zinc Fingers / Q000235:genetics
D019208:Brain-Derived Neurotrophic Factor; D004268:DNA-Binding Proteins; D051766:Early Growth Response Protein 1; C486110:Egr1 protein, rat; D017874:Immediate-Early Proteins; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins; D016760:Proto-Oncogene Proteins c-fos; D016755:Proto-Oncogene Proteins c-jun; D012333:RNA, Messenger; D014157:Transcription Factors; D016291:Dizocilpine Maleate