title: The shuttling of dolichol between VLDL and HDL: involvement of a protein factor from lipoprotein-deficient human serum.
abstract: The occurrence of a dolichol transfer factor in LPDS has been demonstrated using three different transfer assays. Applying a three step purification procedure, the transfer factor could be enriched 4000-5000-fold with a recovery of 1-2%. SDS-gel electrophoresis revealed a molecular weight of 64 kDa. Kinetics as well as the influence of a series of effectors were studied. Transfer was not accompanied by a concurrent esterification and the HDL3 subpopulation showed the highest acceptor capacity. The transfer factor also affected liposomal stability based on calcein fluorescence dequenching upon release. The characteristics of this dol-TP are discussed in view of these other plasma LTPs. Dol-TP might play a role in dolichol transfer from VLDL to HDL, observed in vivo.
authors.affiliation: Department of Medicine, University of Antwerp, Belgium.
authors.forename: G
authors.initials: G
authors.lastname: Van Dessel
authors.forename: A
authors.initials: A
authors.lastname: Lagrou
authors.forename: H J
authors.initials: HJ
authors.lastname: Hilderson
authors.forename: W
authors.initials: W
authors.lastname: Dierick
mesh_terms: D002352:Carrier Proteins / Q000302:isolation & purification*; D004286:Dolichols / Q000378:metabolism*; D006801:Humans; D008074:Lipoproteins / Q000097:blood / Q000172:deficiency* / Q000302:isolation & purification; D008075:Lipoproteins, HDL / Q000378:metabolism*; D008079:Lipoproteins, VLDL / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; D004286:Dolichols; D008074:Lipoproteins; D008075:Lipoproteins, HDL; D008079:Lipoproteins, VLDL; C030363:lipid transfer protein