Binding of dog immunoglobulins G, A, M, and E to concanavalin A.
The binding of dog immunoglobulins G, A, M and E to concanavalin A (Con A) has been investigated. A passive cutaneous anaphylaxis test was used for measurement of dog IgE, and enzyme-linked immunosorbent assay was used for measurement of dog IgG, IgA and IgM. After the dog serum fraction was applied to a Con A-Sepharose column, sequential elution with different buffers was performed; 100% of IgE and IgM, 60% of IgG and 58% of IgA bound to the Con A-Sepharose. IgE was eluted by mannose, methylglucose, and methylmannoside. IgG was eluted by glucose, mannose, methylglucose, and methylmannoside. IgA and IgM were eluted by methylmannoside only. This provides a useful technique in the purification of dog immunoglobulins, especially dog IgE.
Peng
Z
Z
Department of Paediatrics, University of Manitoba, Winnipeg, Canada.
Arthur
G
G
Simons
F E
FE
Becker
A B
AB
D000818:Animals; D002846:Chromatography, Affinity / Q000662:veterinary; D002850:Chromatography, Gel; D003208:Concanavalin A / Q000378:metabolism; D004283:Dog Diseases / Q000276:immunology*; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary; D007136:Immunoglobulins / Q000032:analysis / Q000302:isolation & purification* / Q000378:metabolism; D010323:Passive Cutaneous Anaphylaxis; D006255:Rhinitis, Allergic, Seasonal / Q000276:immunology / Q000662:veterinary*; D012680:Sensitivity and Specificity
D007136:Immunoglobulins; D003208:Concanavalin A