Characterization of a protein that binds multiple sequences in mammalian type C retrovirus enhancers.
Mammalian type C retrovirus enhancer factor 1 (MCREF-1) is a nuclear protein that binds several directly repeated sequences (CNGGN6CNGG) in the Moloney and Friend murine leukemia virus (MLV) enhancers (N. R. Manley, M. O'Connell, W. Sun, N. A. Speck, and N. Hopkins, J. Virol. 67:1967-1975, 1993). In this paper, we describe the partial purification of MCREF-1 from calf thymus nuclei and further characterize the binding properties of MCREF-1. MCREF-1 binds four sites in the Moloney MLV enhancer and three sites in the Friend MLV enhancer. Ethylation interference analysis suggests that the MCREF-1 binding site spans two adjacent minor grooves of DNA.
Sun
W
W
Department of Biochemistry, Dartmouth Medical School, Hanover, New Hampshire 03755.
O'Connell
M
M
Speck
N A
NA
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002417:Cattle; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D004742:Enhancer Elements, Genetic; D005622:Friend murine leukemia virus / Q000235:genetics*; D008969:Molecular Sequence Data; D008979:Moloney murine leukemia virus / Q000235:genetics*; D009687:Nuclear Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D011489:Protein Denaturation; D013950:Thymus Gland / Q000737:chemistry
D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D009838:Oligodeoxyribonucleotides; C080101:gammaretrovirus enhancer factor 1