title: Jun family members are controlled by a calcium-regulated, cyclosporin A-sensitive signaling pathway in activated T lymphocytes.
abstract: The octamer-binding transcription factor Oct-1 is involved in a wide variety of cellular processes but appears to lack a strong transcriptional activation domain, suggesting that it functions in the context of other proteins. We demonstrated previously that Oct-1, in association with a 40-kD protein, OAP40, contributes to the induction of interleukin-2 (IL-2), an early activation gene and major growth factor for T lymphocytes. Here we report that amino acid sequences obtained from purified OAP40 are identical to regions within JunD and c-Jun. We demonstrate that each of these Jun family members can participate in a complex that includes Oct-1 and a regulatory element in the IL-2 enhancer. In transient transfections, both JunD and c-Jun can contribute to activation-specific transcription mediated by this antigen receptor response element. These studies reveal a role, distinct from AP-1 activity, for Jun family members that is controlled by a calcium-triggered, cyclosporin A-sensitive mechanism.
authors.affiliation: Howard Hughes Medical Institute, Department of Pathology, Beckman Center, Stanford University, California 94305.
authors.forename: K S
authors.initials: KS
authors.lastname: Ullman
authors.forename: J P
authors.initials: JP
authors.lastname: Northrop
authors.forename: A
authors.initials: A
authors.lastname: Admon
authors.forename: G R
authors.initials: GR
authors.lastname: Crabtree
mesh_terms: D000595:Amino Acid Sequence; D002118:Calcium / Q000494:pharmacology*; D016572:Cyclosporine / Q000494:pharmacology*; D004268:DNA-Binding Proteins / Q000235:genetics; D004742:Enhancer Elements, Genetic; D005786:Gene Expression Regulation; D051863:Host Cell Factor C1; D007376:Interleukin-2 / Q000235:genetics*; D008213:Lymphocyte Activation / Q000187:drug effects; D008969:Molecular Sequence Data; D050811:Octamer Transcription Factor-1; D010703:Phorbol Esters / Q000494:pharmacology; D016755:Proto-Oncogene Proteins c-jun / Q000235:genetics* / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects; D013601:T-Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics; D014158:Transcription, Genetic
chemical_list: D004268:DNA-Binding Proteins; D051863:Host Cell Factor C1; D007376:Interleukin-2; C079743:Oct-1-associated protein 40; D050811:Octamer Transcription Factor-1; D010703:Phorbol Esters; D016755:Proto-Oncogene Proteins c-jun; D014157:Transcription Factors; D016572:Cyclosporine; D002118:Calcium