Vaccinia virus-specific CD8+ cytotoxic T lymphocytes in humans.
Stimulation of human vaccinia virus immune peripheral blood mononuclear cells in vitro from vaccinia virus-immune donors with live vaccinia virus-infected autologous cells generated vaccinia virus-specific cytotoxic T lymphocytes (CTL) capable of lysing vaccinia virus-infected cells. We generated vaccinia virus-specific CD8+ clones and CD4+ CTL lines by limiting dilution from two donors by using peripheral blood mononuclear cells obtained 2 months or 4 years postrevaccination with vaccinia virus. These results demonstrate that vaccinia virus-specific CTL are generated as a result of immunization of humans with vaccinia virus and that both CD8(+)- and CD4(+)-specific T cells are maintained as memory cells.
Demkowicz
W E
WE
Department of Medicine, University of Massachusetts Medical Center, Worcester 01655.
Ennis
F A
FA
D000911:Antibodies, Monoclonal; D016827:CD8 Antigens / Q000276:immunology*; D002478:Cells, Cultured; D002999:Clone Cells; D003602:Cytotoxicity, Immunologic; D006680:HLA Antigens; D006801:Humans; D007111:Immunity, Cellular; D007156:Immunologic Memory; D008213:Lymphocyte Activation; D010641:Phenotype; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014616:Vaccinia virus / Q000235:genetics / Q000276:immunology*
D000911:Antibodies, Monoclonal; D016827:CD8 Antigens; D006680:HLA Antigens