Incorporation of arachidonic acid in a human cancer gastric tumor cell line (HGT) at various stages of cell proliferation.
Human cancer gastric cells (HGT) in culture were labeled with [14C]arachidonic acid. The kinetic of acylation of arachidonic acid was similar using cells after 3, 5 and 7 days of culture. However, the duration of the proliferation enhanced the acyltransfer to triglycerides and inhibited the transfer to phospholipids. After 8 h of incubation with [14C]arachidonic acid the radioactivity was predominantly recovered in the phosphatidylcholine and phosphatidylethanolamine species. Prelabeling-chase experiments indicated a preferential incorporation of arachidonic acid into phosphatidylcholine followed by a transfer of arachidonate to phosphatidylethanolamine.
Denizot
Y
Y
CNRS URA 1485, Faculté de Médecine, Limoges, France.
Najid
A
A
Rigaud
M
M
D000215:Acylation; D016718:Arachidonic Acid / Q000378:metabolism*; D002455:Cell Division; D006801:Humans; D008563:Membrane Lipids / Q000378:metabolism; D010713:Phosphatidylcholines / Q000378:metabolism; D010714:Phosphatidylethanolamines / Q000378:metabolism; D013274:Stomach Neoplasms / Q000378:metabolism*; D013997:Time Factors; D014280:Triglycerides / Q000378:metabolism; D014407:Tumor Cells, Cultured
D008563:Membrane Lipids; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D014280:Triglycerides; D016718:Arachidonic Acid