The effects of Ca++ on the preservation of myocardial energy and function with University of Wisconsin solution. A 31P nuclear magnetic resonance study of isolated blood perfused Langendorff pig hearts.
We have studied the effects of adding 0.5 mmol/L CaCl2 to University of Wisconsin solution (0.08 mmol/L free Ca++) on hypothermic heart preservation. Isolated pig hearts were subjected to 8 hours of preservation at 12 degrees C; eight hearts were arrested with Ca++ free University of Wisconsin solution, and seven hearts were arrested with Ca(++)-containing University of Wisconsin solution. The recovery of contractile function was evaluated by measuring isovolumic ventricular pressure development. 31P nuclear magnetic resonance spectroscopy was used to monitor the changes in high-energy phosphates. Compared to the hearts arrested with the Ca(++)-free University of Wisconsin solution, the heart arrested with the Ca(++)-containing University of Wisconsin solution showed significantly improved (p < 0.001) contractile functional recovery. No "stone heart" or loss of high-energy phosphates was observed on reperfusion. The hearts showed an increase in diastolic pressure during infusion of the Ca(++)-containing University of Wisconsin solution, however, to show the relationship between the addition of calcium and the increase in diastolic pressure, a second protocol was performed. A 30-minute period of ischemia was induced in thirteen hearts that were arrested at 12 degrees C with either Ca(++)-containing University of Wisconsin solution (n = 8) or Ca(++)-free University of Wisconsin solution (n = 5). Diastolic pressure was monitored during ischemia while ventricular volume was maintained constant with a balloon. The hearts arrested with the Ca(++)-containing University of Wisconsin solution showed a mean rise of 5 mm Hg in diastolic pressure and a rapid decline of phosphocreatine (p < 0.001). Our results suggest that, although 0.08 mmol/L free Ca++ improves functional recovery after 8 hours of heart preservation with University of Wisconsin solution at 12 degrees C, it can increase diastolic pressure during ischemia and accelerate breakdown of the high-energy phosphate stores in the myocardium, suggesting that use of University of Wisconsin solution containing 0.5 mmol/L CaCl2 may result in a significant increase in the intracellular calcium level.
Tian
G
G
Department of Physiology, Faculty of Medicine, University of Ottawa, Ontario, Canada.
Biro
G P
GP
Butler
K W
KW
Xiang
B
B
Vu
C
C
Deslauriers
R
R
D000241:Adenosine; D000493:Allopurinol; D000818:Animals; D002118:Calcium / Q000008:administration & dosage*; D002314:Cardioplegic Solutions; D004734:Energy Metabolism; D005978:Glutathione; D006321:Heart / Q000502:physiology*; D006324:Heart Arrest, Induced; D007328:Insulin; D009682:Magnetic Resonance Spectroscopy; D009200:Myocardial Contraction / Q000187:drug effects; D009206:Myocardium / Q000378:metabolism; D019188:Organ Preservation Solutions; D010710:Phosphates / Q000378:metabolism; D011887:Raffinose; D012996:Solutions; D013552:Swine; D016277:Ventricular Function, Left / Q000187:drug effects
D002314:Cardioplegic Solutions; D007328:Insulin; D019188:Organ Preservation Solutions; D010710:Phosphates; D012996:Solutions; C055206:University of Wisconsin-lactobionate solution; D000493:Allopurinol; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose; D002118:Calcium