title: A modified unique site elimination plasmid mutagenesis procedure utilizing standard calcium chloride transformations.
authors.affiliation: Wadsworth Center for Laboratories and Research, New York State Department of Health, Albany 12201-0509.
authors.forename: J T
authors.initials: JT
authors.lastname: Moore
authors.forename: G F
authors.initials: GF
authors.lastname: Maley
authors.forename: F
authors.initials: F
authors.lastname: Maley
mesh_terms: D002122:Calcium Chloride; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D004587:Electrophoresis, Agar Gel; D004926:Escherichia coli / Q000235:genetics; D005069:Evaluation Studies as Topic; D005821:Genetic Techniques; D016297:Mutagenesis, Site-Directed; D010957:Plasmids; D014170:Transformation, Genetic
chemical_list: D004269:DNA, Bacterial; D002122:Calcium Chloride