Increased production of endothelins in the hippocampus of stroke-prone spontaneously hypertensive rats following transient forebrain ischemia: histochemical evidence.
1. The effect of transient forebrain ischemia on endothelin-1 (ET-1) and endothelin-3 (ET-3) production in the hippocampus of stroke-prone spontaneously hypertensive rats (SHRSPs) was investigated using immunohistochemical techniques. 2. In SHRSPs subjected to 10-min bilateral carotid occlusion, neuronal degeneration in the CA1 pyramidal cell layer of the hippocampus was detectable at 4 days and remarkable at 7 days after reperfusion. 3. Coinciding with neuronal degeneration, ET-1- and ET-3-like immunoreactivities were intense in the CA1 pyramidal-cell layer, the stratum lacunosum moleculare, and the CA4 subfield of the hippocampus. Almost all of the immunostained cells had morphological characteristics of astrocytes. 4. The possibility that ET has a role in the development of neuronal cell death following transient forebrain ischemia warrants further attention.
Yamashita
K
K
Department of Pharmacology 2, Nagasaki University School of Medicine, Japan.
Kataoka
Y
Y
Niwa
M
M
Shigematsu
K
K
Himeno
A
A
Koizumi
S
S
Taniyama
K
K
D000818:Animals; D002545:Brain Ischemia / Q000150:complications / Q000378:metabolism* / Q000473:pathology; D002561:Cerebrovascular Disorders / Q000235:genetics; D016232:Endothelins / Q000096:biosynthesis*; D020022:Genetic Predisposition to Disease; D006624:Hippocampus / Q000378:metabolism* / Q000473:pathology; D006973:Hypertension / Q000150:complications* / Q000235:genetics / Q000378:metabolism; D008297:Male; D009410:Nerve Degeneration; D016548:Prosencephalon / Q000098:blood supply*; D051381:Rats; D011918:Rats, Inbred SHR / Q000378:metabolism*
D016232:Endothelins