Leukemogenicity of v-myb-transformed monoblasts cells can be modulated by normal bone marrow environment.
The avian myeloblastosis virus (AMV) causes monoblastic leukemia in the chick. Two non-producer clones of AMV-transformed monoblasts, BM2/C3A and BM2L/A2B5, have been described (see Bottazzi et al., this issue). They differ in their growth requirements and in their ability to induce leukemia when injected into the chick embryo. We first genetically tagged these clones by retroviral infection with a vector expressing the bacterial lacZ gene. Then, we injected the lacZ-positive cells via the chorioallantoic vein into chick embryos. With BM2L/A2B5 cells, the bone marrow of the injected birds was rapidly invaded by lacZ-positive cells. In addition, these cells rapidly overgrew cultures of bone marrow cells derived from injected animals. Conversely, the growth of BM2/C3A was inhibited in the injected animals and only a few blue cells, with the morphology of macrophages, were detected in cultures of bone marrow cells. We developed an in vitro assay to mimic in vitro the differential growth of BM2/C3A and BM2L/A2B5 observed in vivo. These data strongly suggest that BM2/C3A cells retain their ability to differentiate into macrophages in the normal bone marrow environment and that BM2L/A2B5 cells differ from BMC/C3A in the loss of this capacity.
Bagnis
C
C
Laboratoire de Biologie Moléculaire et Cellulaire, UMR 13 CNRS, Ecole Normale Supérieure de Lyon, France.
Cosset
F L
FL
Samarut
J
J
Moscovici
G
G
Moscovici
C
C
D000818:Animals; D001853:Bone Marrow / Q000502:physiology*; D002450:Cell Communication; D002455:Cell Division; D002461:Cell Line, Transformed; D002471:Cell Transformation, Neoplastic; D002642:Chick Embryo; D007763:Lac Operon; D007948:Leukemia, Monocytic, Acute / Q000209:etiology / Q000235:genetics*; D020626:Oncogene Proteins v-myb; D009857:Oncogenes; D015682:Retroviridae Proteins, Oncogenic / Q000235:genetics*
D020626:Oncogene Proteins v-myb; D015682:Retroviridae Proteins, Oncogenic