The GenePrint Light method for Southern transfer and chemiluminescent detection of human genomic DNA.
A simple, fast and sensitive method to perform Southern transfer and hybridization with nonradioactive detection of genetic loci is described. With a 10-ng sample of human genomic DNA, alleles of the D2S44 locus can be detected within 7 h of completing gel electrophoresis.
Sprecher
C J
CJ
R&D Department, Promega Corporation, Madison, WI 53711.
Liss
L R
LR
Schumm
J W
JW
D015139:Blotting, Southern / Q000379:methods*; D002460:Cell Line; D004247:DNA / Q000032:analysis*; D015252:Deoxyribonucleases, Type II Site-Specific; D006801:Humans; D008163:Luminescent Measurements; D009691:Nucleic Acid Denaturation; D009693:Nucleic Acid Hybridization / Q000379:methods; D014466:Ultraviolet Rays
D004247:DNA; D015252:Deoxyribonucleases, Type II Site-Specific; C024403:GGCC-specific type II deoxyribonucleases