Non-enzymatic glycation of histones.
2-(2-Furoyl)-5-(2-furanyl)-1H-imidazole (FFI) is detected in total histones and the lysine-rich histone 1 fraction isolated from calf thymus after in vitro glycation and hydrolysis with HCl, using gas chromatographic/mass spectrometric analysis. The finding suggests that glucose is bound to the epsilon-amino groups of the lysine residues of the histones. No FFI is monitored in native histone samples.
Liebich
H M
HM
Medizinische Universitätsklinik, Tübingen, Germany.
Gesele
E
E
Wirth
C
C
Wöll
J
J
Jobst
K
K
Lakatos
A
A
D000818:Animals; D002417:Cattle; D008401:Gas Chromatography-Mass Spectrometry; D005947:Glucose / Q000737:chemistry*; D006657:Histones / Q000737:chemistry* / Q000302:isolation & purification; D006868:Hydrolysis; D007093:Imidazoles; D007202:Indicators and Reagents; D010084:Oxidation-Reduction; D013950:Thymus Gland / Q000737:chemistry
D006657:Histones; D007093:Imidazoles; D007202:Indicators and Reagents; C041306:2-(2-furoyl)-4(5)-(2-furanyl)-1H-imidazole; D005947:Glucose