Co-localization of two calcium binding proteins in GABA cells of rat piriform cortex.
In the rat piriform cortex, double-fluorescence immunocytochemistry was used to study the coexistence of two calcium binding proteins, parvalbumin and calbindinD28k, and GABA. Layer I cells were immunoreactive only for GABA. In layer II a small number of cells were immunoreactive for all three substances. A few other cells contained only parvalbumin and GABA immunoreactivity. In layer III a subpopulation of calbindinD28k immunoreactive cells (about 60%) were also immunoreactive for both parvalbumin and GABA. The remainder showed only calbindinD28k and GABA immunoreactivity. Many immunoreactive terminals, which surrounded non-immunoreactive cell somata to form basket endings in layers II and III, were immunoreactive for all three substances. Their major source may be the non-pyramidal neurons in layer III, which contained the three chemical substances.
Kubota
Y
Y
Laboratory for Neural Systems, RIKEN, Saitama, Japan.
Jones
E G
EG
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D064026:Calbindins; D002135:Calcium-Binding Proteins / Q000378:metabolism*; D002540:Cerebral Cortex / Q000033:anatomy & histology / Q000166:cytology / Q000378:metabolism*; D003429:Cross Reactions; D007150:Immunohistochemistry; D008297:Male; D009412:Nerve Fibers / Q000276:immunology / Q000378:metabolism; D009474:Neurons / Q000378:metabolism / Q000502:physiology*; D010320:Parvalbumins / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D064030:S100 Calcium Binding Protein G / Q000378:metabolism; D005680:gamma-Aminobutyric Acid / Q000502:physiology*
D000911:Antibodies, Monoclonal; D064026:Calbindins; D002135:Calcium-Binding Proteins; D010320:Parvalbumins; D064030:S100 Calcium Binding Protein G; D005680:gamma-Aminobutyric Acid