title: Structure and expression of hybrid dysgenesis-induced alleles of the ovarian tumor (otu) gene in Drosophila melanogaster.
abstract: Mutations at the ovarian tumor (otu) gene of Drosophila melanogaster cause female sterility and generate a range of ovarian phenotypes. Quiescent (QUI) mutants exhibit reduced germ cell proliferation; in oncogenic (ONC) mutants germ cells undergo uncontrolled proliferation generating excessive numbers of undifferentiated cells; the egg chambers of differentiated (DIF) mutants differentiate to variable degrees but fail to complete oogenesis. We have examined mutations caused by insertion and deletion of P elements at the otu gene. The P element insertion sites are upstream of the major otu transcription start sites. In deletion derivatives, the P element, regulatory regions and/or protein coding sequences have been removed. In both insertion and deletion mutants, the level of otu expression correlates directly with the severity of the phenotype: the absence of otu function produces the most severe QUI phenotype while the ONC mutants express lower levels of otu than those which are DIF. The results of this study demonstrate that the diverse mutant phenotypes of otu are the consequence of different levels of otu function.
authors.affiliation: Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill 27599-3280.
authors.forename: G L
authors.initials: GL
authors.lastname: Sass
authors.forename: J D
authors.initials: JD
authors.lastname: Mohler
authors.forename: R C
authors.initials: RC
authors.lastname: Walsh
authors.forename: L J
authors.initials: LJ
authors.lastname: Kalfayan
authors.forename: L L
authors.initials: LL
authors.lastname: Searles
mesh_terms: D000483:Alleles; D000818:Animals; D001483:Base Sequence; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000235:genetics*; D005260:Female; D005786:Gene Expression Regulation; D005796:Genes; D006824:Hybridization, Genetic / Q000235:genetics*; D007247:Infertility, Female / Q000235:genetics; D007301:Insect Hormones / Q000235:genetics*; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D009857:Oncogenes; D009866:Oogenesis / Q000235:genetics; D010051:Ovarian Neoplasms / Q000235:genetics*; D010641:Phenotype; D017384:Sequence Deletion
chemical_list: D029721:Drosophila Proteins; D007301:Insect Hormones; C061992:otu protein, Drosophila