title: UVB radiation exposes fibrinogen binding sites on platelets by activating protein kinase C via reactive oxygen species.
abstract: Previous studies have shown that ultraviolet B (UVB) radiation causes platelet aggregation by exposing fibrinogen binding sites via activation of an intracellular mechanism. In the present study we have further investigated the routes of platelet activation following UVB exposure. Evidence is provided that UVB radiation does not activate the platelets via the classical Phospholipase A2 and Phospholipase C routes. Despite this observation, UVB-induced fibrinogen binding was found to be correlated with a 40% increase in phosphorylated 47 kD protein. Both findings could be completely inhibited in the presence of staurosporine, a potent inhibitor of protein kinase C (PK-C). In efforts to explain the mechanism of PK-C activation by UV radiation we found that both UV-induced PK-C activation and platelet aggregation were significantly reduced in the presence of specific scavengers for reactive oxygen species including superoxide dismutase and catalase. We conclude that exposure of platelets to UVB radiation can activate PK-C via oxygen radicals, resulting in exposure of fibrinogen binding sites and subsequent platelet aggregation.
authors.affiliation: Department of Haematology, University Hospital Utrecht, The Netherlands.
authors.forename: M
authors.initials: M
authors.lastname: van Marwijk Kooy
authors.forename: J W
authors.initials: JW
authors.lastname: Akkerman
authors.forename: S
authors.initials: S
authors.lastname: van Asbeck
authors.forename: L
authors.initials: L
authors.lastname: Borghuis
authors.forename: H C
authors.initials: HC
authors.lastname: van Prooijen
mesh_terms: D001665:Binding Sites; D001792:Blood Platelets / Q000201:enzymology; D004789:Enzyme Activation / Q000528:radiation effects; D005340:Fibrinogen / Q000378:metabolism*; D006801:Humans; D015539:Platelet Activation / Q000528:radiation effects*; D010974:Platelet Aggregation; D011493:Protein Kinase C / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism; D014466:Ultraviolet Rays
chemical_list: D017382:Reactive Oxygen Species; D005340:Fibrinogen; D011493:Protein Kinase C