title: CBEIA: programs for simulation of ELISA experiments and affinity determination.
abstract: Two computer programs for the analysis of results of enzyme immunoassays are presented. CBEIA-C simulates ELISA experiments based on competition between immobilized and soluble antibody for a soluble antigen (or vice versa). CBEIA-S simulates the formation of a ternary complex of immobilized antibody, antigen and soluble antibody. The programs provide a more reliable basis for affinity determination than linearized plots. Results obtained by the application of these programs are described in the accompanying paper on pp. 129-133).
authors.affiliation: Department of Medical Immunology, Humboldt University Berlin, Medical School (Charité), Germany.
authors.forename: R W
authors.initials: RW
authors.lastname: Glaser
mesh_terms: D000915:Antibody Affinity / Q000276:immunology*; D003198:Computer Simulation; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D012984:Software