Effect of gamma irradiation on reactivity of rinderpest virus antigen with bovine immune serum in enzyme-linked immunosorbent assays and virus neutralization and indirect fluorescent-antibody tests.
Gamma irradiation effectively inactivated gradient-purified rinderpest virus. Irradiated antigen and sera remained functional in enzyme-linked immunosorbent assays, virus neutralization tests, and indirect fluorescent-antibody tests. Irradiation, however, led to a dose-dependent decrease in reactivity, particularly significant (P < 0.05) when both reagents were irradiated. To avoid false-positive reactions, only one reagent (serum or antigen) may be irradiated.
Saliki
J T
JT
Foreign Animal Disease Diagnostic Laboratory, USDA/APHIS/VS/NVSI, Greenport, New York 11944.
Berninger
M L
ML
Torres
A
A
House
J A
JA
Mebus
C A
CA
Dubovi
E J
EJ
D000818:Animals; D000914:Antibodies, Viral / Q000528:radiation effects; D000956:Antigens, Viral / Q000528:radiation effects*; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D005720:Gamma Rays; D009500:Neutralization Tests; D012301:Rinderpest / Q000175:diagnosis; D012302:Rinderpest virus / Q000276:immunology* / Q000528:radiation effects; D014773:Virology / Q000379:methods
D000914:Antibodies, Viral; D000956:Antigens, Viral