Fertile asymmetric somatic hybrids between Lycopersicon esculentum Mill. and Lycopersicon peruvianum var. dentatum Dun.
Thirteen nuclear asymmetric hybrids were regenerated under selective conditions following fusion of chlorophyll-deficient protoplasts from cultivated tomato (Lycopersicon esculentum Mill.) and gamma-irradiated protoplasts from the wild species Lycopersicon peruvianum var. dentatum Dun. All hybrid plants were classified as being asymmetric based on morphological traits, chromosome numbers and isozyme patterns. The majority of the hybrids inherited Lycopersicon peruvianum var. dentatum chloroplasts. Mitochondrial DNA analysis revealed mixed mitochondrial populations deriving from both parents in some of the hybrids and rearranged mitochondrial DNA in others. The asymmetric hybrids express some morphological traits that are not found in either of the parental species. Fertile F1 plants were obtained after self-pollination of the asymmetric hybrids in four cases. The results obtained confirm the potential of asymmetric hybridization as a new source of genetic variation, and as a method for transferring of a part of genetic material from donor to recipient, and demonstrate that it is possible to produce fertile somatic hybrids by this technique.
Ratushnyak
Y I
YI
Institute of Cell Biology and Genetic Engineering, Ukrainian Academy of Sciences, Kiev.
Cherep
N N
NN
Zavgorodnyaya
A V
AV
Latypov
S A
SA
Borozenko
I V
IV
Rachkovskaya
R I
RI
Gleba
Y Y
YY
D000135:Acid Phosphatase / Q000378:metabolism; D002459:Cell Fusion; D002736:Chloroplasts / Q000502:physiology; D002875:Chromosomes; D003433:Crosses, Genetic; D004247:DNA / Q000378:metabolism; D004272:DNA, Mitochondrial / Q000235:genetics; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D004950:Esterases / Q000378:metabolism; D005111:Extrachromosomal Inheritance; D005298:Fertility; D005720:Gamma Rays; D006822:Hybrid Cells; D007527:Isoenzymes; D010945:Plants, Edible / Q000196:embryology / Q000235:genetics* / Q000528:radiation effects; D011003:Ploidies; D011058:Pollen; D011523:Protoplasts / Q000528:radiation effects
D004272:DNA, Mitochondrial; D007527:Isoenzymes; D004247:DNA; D004950:Esterases; D015252:Deoxyribonucleases, Type II Site-Specific; D000135:Acid Phosphatase