title: Covalently immobilized DNA plate for luminometric DNA-DNA hybridization to identify viridans streptococci in under 2 hours.
abstract: Single-stranded chromosomal DNA was covalently bound to a microdilution plate and used for quantitative DNA-DNA hybridization. After 30 min, hybridized DNA was quantitatively detected by alkaline phosphatase and a chemiluminescent substrate. This method was successfully used for the rapid identification of viridans streptococci.
authors.affiliation: Department of Microbiology, Gifu University, School of Medicine, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Adnan
authors.forename: N
authors.initials: N
authors.lastname: Li
authors.forename: H
authors.initials: H
authors.lastname: Miura
authors.forename: Y
authors.initials: Y
authors.lastname: Hashimoto
authors.forename: H
authors.initials: H
authors.lastname: Yamamoto
authors.forename: T
authors.initials: T
authors.lastname: Ezaki
mesh_terms: D004269:DNA, Bacterial / Q000032:analysis*; D008163:Luminescent Measurements; D009693:Nucleic Acid Hybridization / Q000379:methods*; D013291:Streptococcus / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D004269:DNA, Bacterial