Glycerophosphocholine release in human erythrocytes. 1H spin-echo and 31P-NMR evidence for lysophospholipase.
The direct techniques of 1H spin-echo and 31P-NMR spectroscopy made it possible to monitor the release of glycerophosphocholine from lysophosphatidylcholine in lysates from human red blood cells. Thus, the existence of a lysophospholipase in human erythrocytes was confirmed using a new more direct method. No evidence for a phospholipase A2 activity in the haemolysates was found with the same approach; since this enzyme is present in leukocytes, the absence of activity helped verify the purity of the erythrocyte preparation. The lysophospholipase may constitute, with the earlier described glycerophosphocholine phosphodiesterase activity, a metabolic unit for the removal of haemolytic lysophosphatidylcholine which is formed in the erythrocyte membranes as well as taken up from the plasma.
Selle
H
H
Department of Biochemistry, University of Sydney, Australia.
Chapman
B E
BE
Kuchel
P W
PW
D004912:Erythrocytes / Q000201:enzymology / Q000378:metabolism*; D005997:Glycerylphosphorylcholine / Q000378:metabolism*; D006801:Humans; D008244:Lysophosphatidylcholines / Q000378:metabolism; D008245:Lysophospholipase / Q000097:blood* / Q000502:physiology; D009682:Magnetic Resonance Spectroscopy; D010741:Phospholipases A / Q000378:metabolism; D054467:Phospholipases A2
D008244:Lysophosphatidylcholines; D005997:Glycerylphosphorylcholine; D010741:Phospholipases A; D054467:Phospholipases A2; D008245:Lysophospholipase