Heinz body hemolytic anemia induced by DQ-2511, a new anti-ulcer drug, in dogs.
DQ-2511, a new anti-ulcer drug, was administered to beagle dogs for 4 weeks to investigate the mechanism whereby this drug induced hemolytic anemia and its reversibility in comparison with beta-acetylphenylhydrazine. Hemolytic anemia accompanied by an increase in the number of cells containing Heinz bodies that was preceded by a marked decrease in blood-reduced glutathione concentration was observed in dogs receiving 600 mg/kg of DQ-2511, but only a slight increase in the methemoglobin level was noted. beta-Acetylphenylhydrazine, however, caused hemolytic anemia accompanied by marked increases in both Heinz body-containing cells and methemoglobin concentration, but the blood-reduced glutathione concentration was not decreased consistently with the formation of Heinz bodies. Hemolytic anemia disappeared after a 4-week recovery period in the dogs that received DQ-2511. These results suggest that decreases in reduced glutathione in erythrocytes play an important role in the anemia and Heinz body formation induced by DQ-2511, but not by beta-acetylphenylhydrazine.
Ohno
H
H
Drug Safety Research Center, Developmental Research Laboratories, Daiichi Pharmaceutical Company Ltd., Tokyo R & D Center, Japan.
Tojo
H
H
Kakihata
K
K
Nomura
M
M
Takayama
S
S
D000284:Administration, Oral; D000743:Anemia, Hemolytic / Q000097:blood / Q000139:chemically induced*; D000818:Animals; D000897:Anti-Ulcer Agents / Q000633:toxicity*; D001549:Benzamides / Q000633:toxicity*; D004285:Dogs; D004305:Dose-Response Relationship, Drug; D004906:Erythrocyte Count / Q000187:drug effects; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism; D005978:Glutathione / Q000097:blood; D006366:Heinz Bodies / Q000187:drug effects*; D006400:Hematocrit; D006454:Hemoglobins / Q000032:analysis / Q000187:drug effects; D008297:Male; D010659:Phenylhydrazines / Q000633:toxicity
D000897:Anti-Ulcer Agents; D001549:Benzamides; D006454:Hemoglobins; D010659:Phenylhydrazines; C058308:ecabapide; C006384:N(1)-acetylphenylhydrazine; D005978:Glutathione