On the toxicity of low doses of tetrasodium-ethylenediamine-tetraacetate (Na-EDTA) in normal rat kidney (NRK) cells in culture.
The toxicity of tetrasodium-ethylenediaminetetraacetate (Na-EDTA) was investigated in normal rat kidney cells (NRK-52E, American Type Culture Collection) in culture. Cell death and reduced colony-forming ability were observed at Na-EDTA concentrations < 100 microM, that is at concentrations lower than that required to chelate all the Ca2+ in the growth medium. No toxicity was observed when cells were exposed to 100 microM Zn- or Fe-EDTA, but the same concentration of Cu-EDTA was as toxic as Na-EDTA. Continuous exposure of the cell cultures to 5 microM Na- or Zn-EDTA for up to 7 weeks yielded no indications of toxicity.
Hugenschmidt
S
S
Kernforschungszentrum Karlsruhe, Institut für Genetik und Toxikologie, Federal Republic of Germany.
Planas-Bohne
F
F
Taylor
D M
DM
D000818:Animals; D002448:Cell Adhesion; D016923:Cell Death / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004247:DNA / Q000378:metabolism; D004492:Edetic Acid / Q000633:toxicity*; D007674:Kidney Diseases / Q000139:chemically induced*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D011506:Proteins / Q000378:metabolism; D051381:Rats; D014343:Trypan Blue
D011506:Proteins; D004247:DNA; D004492:Edetic Acid; D007770:L-Lactate Dehydrogenase; D014343:Trypan Blue