title: Characterization of vasopressin-mediated GSH efflux from Hep G2 cells: significance of protein kinase C.
abstract: Vasopressin stimulated GSH efflux from Hep G2 cells. The maximal effect was observed at 10nM. Pretreatment with pertussis toxin or cholera toxin for 18 hr increased GSH efflux. Vasopressin-mediated GSH efflux was observed even in the cells pretreated with those compounds. Dibutyryl-cAMP or dibutyryl-cGMP enhanced GSH efflux although an additive effect of vasopressin was not observed. Glucagon and a phorbol ester independently increased GSH efflux while both compounds decreased the effect of vasopressin. Staurosporine, an inhibitor of protein kinase C, inhibited vasopressin-mediated GSH efflux. The effect of vasopressin was observed even in the absence of extracellular Ca2+. Vasopressin stimulates GSH efflux from Hep G2 cells and protein kinase C-dependent pathway may play a significant role in vasopressin-mediated GSH efflux.
authors.affiliation: Department of Public Health and Environmental Sciences, Faculty of Medicine, Tokyo Medical and Dental University, Japan.
authors.forename: J H
authors.initials: JH
authors.lastname: Liu
authors.forename: C
authors.initials: C
authors.lastname: Sato
authors.forename: T
authors.initials: T
authors.lastname: Takano
authors.forename: F
authors.initials: F
authors.lastname: Marumo
mesh_terms: D000470:Alkaloids / Q000494:pharmacology; D001127:Arginine Vasopressin / Q000502:physiology*; D005978:Glutathione / Q000378:metabolism*; D006801:Humans; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000378:metabolism*; D019311:Staurosporine; D014407:Tumor Cells, Cultured
chemical_list: D000470:Alkaloids; D001127:Arginine Vasopressin; D011493:Protein Kinase C; D005978:Glutathione; D019311:Staurosporine