Increased expression of PDGF A- and B-chain genes in rat lungs with hypoxic pulmonary hypertension.
To study the molecular basis of vascular remodeling in pulmonary hypertension, we developed an experimental system in which male Sprague-Dawley rats were exposed to hypoxia for up to 3 wk. Both the right ventricular systolic pressure and gravimetric index for right ventricular hypertrophy were higher in rats exposed to hypoxia for 3 wk than those of age-matched control rats (P < 0.01), indicating that pulmonary hypertension was established under conditions used. To examine the possible involvement of platelet-derived growth factor (PDGF) in the pulmonary vascular remodeling caused by hypoxia, we cloned rat PDGF A- and B-chain cDNA and prepared specific cRNA probes. Northern blot analysis revealed that PDGF B-chain mRNA levels in the lungs were increased, reached a maximum of day 1, and were sustained at day 3, whereas PDGF A-chain mRNA levels reached a maximum on day 3. Thus the increase in the PDGF B-chain mRNA level precedes that in the PDGF A-chain mRNA level. These results suggest that the PDGF A- and B-chain products may be coordinately and sequentially involved in hypoxic pulmonary vascular remodeling.
Katayose
D
D
First Department of Internal Medicine, Tohoku University School of Medicine, Miyagi, Japan.
Ohe
M
M
Yamauchi
K
K
Ogata
M
M
Shirato
K
K
Fujita
H
H
Shibahara
S
S
Takishima
T
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D015870:Gene Expression; D006439:Hemodynamics; D006976:Hypertension, Pulmonary / Q000209:etiology* / Q000235:genetics* / Q000503:physiopathology; D000860:Hypoxia / Q000150:complications* / Q000235:genetics; D008168:Lung / Q000502:physiology*; D008297:Male; D015335:Molecular Probes / Q000235:genetics; D008969:Molecular Sequence Data; D010982:Platelet-Derived Growth Factor / Q000235:genetics*; D016133:Polymerase Chain Reaction; D051381:Rats; D017207:Rats, Sprague-Dawley
D015335:Molecular Probes; D010982:Platelet-Derived Growth Factor; C075278:platelet-derived growth factor A; D004247:DNA