title: Establishment and characterization of 7 ovarian carcinoma cell lines and one granulosa tumor cell line: growth features and cytogenetics.
abstract: The characteristics of 7 newly established ovarian carcinoma cell lines and one granulosa tumor cell line obtained from tumor samples of 7 patients with varying histology of the primary tumor are reported. The cell lines were isolated from 5 serous carcinomas, a mucinous carcinoma, an endometrioid carcinoma and a granulosa cell tumor. All cell lines were passaged at least 25 times and showed stable growth rates. Colony-forming efficiency varied on plastic from 2 to 57% and in agar from 0.01 to 9.30%. The DNA index of the granulosa tumor cell line was diploid, while the ovarian carcinoma cell lines were all aneuploid. In 2 cell lines polyploidisation occurred during culturing. A thorough cytogenetic analysis of 7 cell lines revealed that the granulosa tumor cell line has only minor cytogenetic abnormalities (+5, 22q+). In contrast, the epithelial ovarian-cancer cell lines gave very complex karyotypes with numerous markers and structurally rearranged chromosomes. The chromosomes most often in excess were 15 and 20. Structural rearrangements of chromosomes 1, 3, 7 and 11 were prominent in all ovarian cell lines. In addition, we found changes in chromosomes X, 5, 8 and 13 that have rarely been described before.
authors.affiliation: Department of Clinical Oncology, University Hospital, Leiden, The Netherlands.
authors.forename: C A
authors.initials: CA
authors.lastname: van den Berg-Bakker
authors.forename: A
authors.initials: A
authors.lastname: Hagemeijer
authors.forename: E M
authors.initials: EM
authors.lastname: Franken-Postma
authors.forename: V T
authors.initials: VT
authors.lastname: Smit
authors.forename: P J
authors.initials: PJ
authors.lastname: Kuppen
authors.forename: H H
authors.initials: HH
authors.lastname: van Ravenswaay Claasen
authors.forename: C J
authors.initials: CJ
authors.lastname: Cornelisse
authors.forename: P I
authors.initials: PI
authors.lastname: Schrier
mesh_terms: D002277:Carcinoma / Q000473:pathology*; D002869:Chromosome Aberrations / Q000473:pathology; D002871:Chromosome Banding; D025063:Chromosome Disorders; D004273:DNA, Neoplasm / Q000032:analysis; D005260:Female; D005434:Flow Cytometry; D006106:Granulosa Cell Tumor / Q000473:pathology*; D006801:Humans; D010051:Ovarian Neoplasms / Q000473:pathology*; D014407:Tumor Cells, Cultured
chemical_list: D004273:DNA, Neoplasm