Effects of chlorpromazine on phosphatidylserine biosynthesis in rat pup brain exposed to ethanol in utero.
Phosphatidylserine biosynthesis in rat pup brain was examined by assaying the serine base-exchange enzyme activity in the microsomal and plasma membrane fractions, and by measuring the incorporation of [3H]serine into phosphatidylserine in brain slices and in the intact brain. Chlorpromazine, either added in vitro into the incubation system or administered to animals via i.p. injection or feeding a liquid diet, gave rise to an increase in the phosphatidylserine biosynthesis activity. Ethanol administered in the form of a liquid diet to pregnant rats (day 11 birth) resulted in a decrease in phosphatidylserine biosynthesis in the newborn and developing brain. The ethanol-induced decrease in phosphatidylserine biosynthetic activity could be reversed by adding chlorpromazine to the ethanol diet. Results demonstrate that phosphatidylserine biosynthesis in the neonatal brain is affected in opposite directions by chlorpromazine and ethanol. This poses the possibility that chlorpromazine administration may be effective in alleviating the deleterious effects caused by the decreased phosphatidylserine biosynthesis in brain due to in utero ethanol exposure.
Rhodes
P G
PG
Departments of Child Health, University of Missouri, Columbia 65212.
Hu
Z Y
ZY
Sun
G Y
GY
D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism*; D001921:Brain / Q000187:drug effects* / Q000196:embryology / Q000378:metabolism; D002462:Cell Membrane / Q000201:enzymology; D002746:Chlorpromazine / Q000494:pharmacology*; D000431:Ethanol / Q000494:pharmacology*; D005260:Female; D007700:Kinetics; D008861:Microsomes / Q000201:enzymology; D019884:Nitrogenous Group Transferases; D010718:Phosphatidylserines / Q000096:biosynthesis*; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D012694:Serine / Q000378:metabolism; D014166:Transferases / Q000378:metabolism
D010718:Phosphatidylserines; D000431:Ethanol; D012694:Serine; D014166:Transferases; D019884:Nitrogenous Group Transferases; C022370:phospholipid serine base exchange enzyme; D002746:Chlorpromazine