title: Damage to the rainbow trout (Oncorhyncus mykiss) lens following an acute dose of UVB.
abstract: Rainbow trout (Oncorhynchus mykiss) were used to model the solar radiation-induced cataract that occurs in hatchery-reared lake trout (Salvelinus namaycush). Rainbow trout eyes were exposed to wideband predominantly UVB radiant energy from a filtered 1000W xenon source. Lenticular damage was assessed and classified and comeal damage noted. Lenticular threshold radiant exposure was 0.48 J.cm-2 which is in the same range as the mammalian lens. Permanent lenticular damage occurred at radiant exposure levels approximately twice the threshold for lenticular radiant exposure (also similar to the mammalian response). Slit-lamp and scanning electron microscopical results support the theory of post-exposure anterior subcapsular epithelial cell damage, lens fibre swelling, and eventual rupture, leading to areas of lower refractive index in the anterior subcapsular region of the lens.
authors.affiliation: Optical Radiation Laboratory, School of Optometry, University of Waterloo, Ontario, Canada.
authors.forename: A P
authors.initials: AP
authors.lastname: Cullen
authors.forename: C A
authors.initials: CA
authors.lastname: Monteith-McMaster
mesh_terms: D000818:Animals; D002386:Cataract / Q000209:etiology* / Q000473:pathology; D004195:Disease Models, Animal; D007908:Lens, Crystalline / Q000528:radiation effects* / Q000648:ultrastructure; D008027:Light; D008452:Maximum Allowable Concentration; D008855:Microscopy, Electron, Scanning; D011829:Radiation Dosage; D014337:Trout; D014466:Ultraviolet Rays / Q000009:adverse effects*