New algorithm for analysis of data obtained by means of circular dichroism titration method.
A new method for analysis of circular dichroism titration data used for drug-protein binding investigations is proposed. A square equation between molar ellipticity change and total drug concentration is obtained which is examined analytically. A new minimization algorithm for determining the binding sites and association constants of each type of drug-protein complexes is applied.
Stavreva
N
N
Department of Physics and Biophysics, Medical Academy, Sofia, Bulgaria.
Ruseva
V
V
Michailova
D
D
Stavrev
P
P
D000465:Algorithms; D002942:Circular Dichroism; D004364:Pharmaceutical Preparations / Q000737:chemistry / Q000378:metabolism; D011485:Protein Binding; D011955:Receptors, Drug / Q000378:metabolism
D004364:Pharmaceutical Preparations; D011955:Receptors, Drug