Prevention of singlet oxygen-induced DNA damage by lipoate.
Among the several biologically and pharmacologically active sulfur compounds examined, only lipoic acid and dihydrolipoic acid provided protection to plasmid DNA against singlet molecular oxygen (1O2). 1O2 was generated in phosphate buffer by the thermal dissociation of the endoperoxide of 3,3'-(1,4-naphthylidene) dipropionate (NDPO2). The protecting effect of lipoic acid was time- and pH-dependent and significant protection was seen even at 50 microM. The antioxidant effect was adversely affected by temperatures above 45 degrees C. Superoxide dismutase and catalase marginally enhanced this effect. Metal chelation with EDTA decreased the protection by lipoate, indicating that metal ions may be involved. The protective effect was diminished when the disulfide was added after single-strand breaks were induced by 1O2. The formation of 8-oxoguanosine from guanosine upon exposure to NDPO2 was not altered by lipoate.
Devasagayam
T P
TP
Institut für Physiologische Chemie I, Universität Düsseldorf, Germany.
Subramanian
M
M
Pradhan
D S
DS
Sies
H
H
D002374:Catalase / Q000494:pharmacology; D004247:DNA / Q000187:drug effects; D004249:DNA Damage / Q000187:drug effects*; D004492:Edetic Acid / Q000494:pharmacology; D005978:Glutathione / Q000494:pharmacology; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D009284:Naphthols / Q000378:metabolism; D010100:Oxygen / Q000494:pharmacology*; D010957:Plasmids; D026082:Singlet Oxygen; D013482:Superoxide Dismutase / Q000494:pharmacology; D008063:Thioctic Acid / Q000494:pharmacology*
D009284:Naphthols; D026082:Singlet Oxygen; D008063:Thioctic Acid; D004247:DNA; C058008:3,3'-(1,4-naphthylidene)diproprionate; D004492:Edetic Acid; D002374:Catalase; D013482:Superoxide Dismutase; D005978:Glutathione; D010100:Oxygen