Cloning and sequencing of glutamate mutase component E from Clostridium tetanomorphum.
The nucleotide sequence of the large subunit E of glutamate mutase of Clostridium tetanomorphum was determined. The protein consists of 483 amino acids and is not made in a precursor form, thus excluding the possibility of subunit E being a pyruvoyl enzyme. It shows no homology to any other protein in the database, and while binding coenzyme B12, a conspicuous B12 binding motif, shared amongst other proteins, is not detectable at the sequence level.
Brecht
M
M
Genzentrum der Universität München, Germany.
Kellermann
J
J
Plückthun
A
A
D000591:Amino Acid Isomerases / Q000737:chemistry / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D003013:Clostridium / Q000201:enzymology* / Q000235:genetics; D003062:Codon; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D019751:Intramolecular Transferases; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
D003062:Codon; D004269:DNA, Bacterial; D000591:Amino Acid Isomerases; D019751:Intramolecular Transferases; C021570:methylaspartate mutase