title: Effect of tracheal insufflation of deferoxamine on acute ozone toxicity in rats.
abstract: To test the hypothesis that deferoxamine (DFO) may protect the lung against the acute toxicity of ozone (O3), male Sprague-Dawley rats (250 to 300 gm) were tracheally insufflated before exposure to O3 (2 ppm for 4 hours) with DFO (25 mg/kg), ferric-DFO, or sterile water. Measurements of bronchoalveolar lavage fluid (BALF) protein, ascorbate, hydrogen peroxide, and lipid hydroperoxides were made immediately after O3 exposure and 12 and 24 hours later. DFO (25 mg/kg) decreased BALF protein concentration (p < 0.05) and was associated with higher BALF ascorbate concentrations (p < 0.01) in O3-exposed animals. Ferric-DFO did not show these protective effects. No peroxides were found in BALF from any control or O3-exposed animals (detection limit: H2O2, 1 pmol; lipid hydroperoxides, 0.3 pmol). Higher DFO doses (50 mg/kg and 100 mg/kg) did not decrease BALF protein concentration immediately after O3 exposure or 12 hours later. Indeed, the highest dose was toxic to O3-exposed animals. Although DFO is able to protect against O3-induced lung damage in rats, apparently by chelating iron, its effect dose range appears to be narrow.
authors.affiliation: Department of Internal Medicine, School of Medicine, University of California, Davis 95616-8542.
authors.forename: S
authors.initials: S
authors.lastname: Louie
authors.forename: M A
authors.initials: MA
authors.lastname: Arata
authors.forename: S D
authors.initials: SD
authors.lastname: Offerdahl
authors.forename: B
authors.initials: B
authors.lastname: Halliwell
mesh_terms: D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry*; D003676:Deferoxamine / Q000008:administration & dosage / Q000378:metabolism / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D007327:Insufflation; D015227:Lipid Peroxidation; D008168:Lung / Q000187:drug effects*; D008297:Male; D010126:Ozone / Q000378:metabolism / Q000633:toxicity*; D011506:Proteins / Q000032:analysis*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D011506:Proteins; D010126:Ozone; D003676:Deferoxamine