Identification of an AP-1 transcription factor binding site within the human cholecystokinin (CCK) promoter.
Neuropeptide gene expression, i.e. proenkephalin A, may be modulated by activating protein (AP-1) transcription factor complexes which associate with specific DNA sequence motifs, known as the AP-1 binding sites. Gel mobility shift assays revealed that nuclear extracts prepared from a human neuroblastoma cell-line, SK-N-MC, bind to the 5'-CTGCGTCAGCG-3' motif, present within the human cholecystokinin (CCK) promoter. In contrast, the mutated 5'-CTGCAACAGCG-3' motif did not bind any specific protein complex. In vitro run-off transcription and Western blot analysis revealed the expression of the protooncogenes Fos and Jun. This suggests that Fos/Jun homo- or heterodimeric complexes may interact with the 5'-CTGCGTCAGCG-3' motif, present within the human CCK promoter.
Monstein
H J
HJ
Department of Clinical Biochemistry, Rigshospitalet, Copenhagen, Denmark.
D000199:Actins / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002766:Cholecystokinin / Q000235:genetics*; D004745:Enkephalins / Q000235:genetics; D015870:Gene Expression; D016762:Genes, fos; D016758:Genes, jun; D006801:Humans; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009447:Neuroblastoma; D015345:Oligonucleotide Probes; D011401:Promoter Regions, Genetic; D011498:Protein Precursors / Q000235:genetics; D016755:Proto-Oncogene Proteins c-jun / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D014407:Tumor Cells, Cultured
D000199:Actins; D004745:Enkephalins; D015345:Oligonucleotide Probes; D011498:Protein Precursors; D016755:Proto-Oncogene Proteins c-jun; C029992:proenkephalin; D002766:Cholecystokinin