Effects of surface electrostimulation on the structure and metabolic properties in monkey skeletal muscle.
Adaptative changes in skeletal muscle following surface electrical stimulation (SES) were investigated in rhesus monkeys. SES was performed on the triceps brachialis muscle (TB) according to an intermittent pattern. The procedure was carried out for 3 wk, using a current with a medium frequency of 60 Hz normally observed in fast motor axons. The histochemical assays performed on biopsies taken from proximal and distal parts of the TB muscle, before and after the SES program, showed that the distribution of fibers typed by ATPase was unaffected. On the other hand, SES led to an overall increase in the mean fiber cross-sectional area (FCSA); P < 0.01 (+13.7%, NS, in proximal portion, vs +31%, P < 0.01 in distal portion). This increase in size occurred in all fiber types. SES was shown to induce an overall increase in capillary to fiber ratio (C/F; +11.06%, NS, in proximal portion, vs +25.93%, P < 0.05 in distal portion). The number of capillaries surrounding fiber Type II (CAFII) was significantly increased by SES (P < 0.05): +3.21%, NS, in proximal portion, versus +21.47%, P < 0.05 in distal portion. Moreover, the number of capillaries surrounding fiber Type I (CAFI) was statistically unaffected by SES. These results suggest that a stimulation of capillary growth may occur following SES-training. Citrate synthase activity was significantly increased after SES. This enhancement in oxidative potential was shown to occur in all fiber types (NADH-diaphorase staining).(ABSTRACT TRUNCATED AT 250 WORDS)
Bigard
A X
AX
Division de Physiologie Métabolique et Hormonale, Centre d'Etudes et de Recherches de Médecine Aérospatiale, CEV, Brétigny/Orge, France.
Lienhard
F
F
Merino
D
D
Serrurier
B
B
Guezennec
C Y
CY
D000222:Adaptation, Physiological; D000704:Analysis of Variance; D000818:Animals; D016009:Chi-Square Distribution; D002950:Citrate (si)-Synthase / Q000378:metabolism; D008058:Dihydrolipoamide Dehydrogenase / Q000378:metabolism; D006651:Histocytochemistry; D008253:Macaca mulatta; D008297:Male; D009119:Muscle Contraction / Q000502:physiology*; D009132:Muscles / Q000033:anatomy & histology* / Q000098:blood supply / Q000201:enzymology*; D010805:Physical Conditioning, Animal; D004561:Transcutaneous Electric Nerve Stimulation
D008058:Dihydrolipoamide Dehydrogenase; D002950:Citrate (si)-Synthase