title: An efficient method for the recovery of 14C in labeling experiments using wheat.
abstract: This report describes a reliable method of 14CO2 recovery from 14C-labeled sucrose applied to leaves of young wheat plants. After completion of the labeling experiment, sections of the plant are harvested, digested in concentrated sulfuric acid and oxidized by 3% hydrogen peroxide in a closed system containing CO2-absorbing 2 N NaOH. Heating the sulfuric acid containing vial to 85 degrees C is necessary to achieve near total recovery of 14C-label within 30 min. This technique can be applied to other plant or animal systems, to study the translocation of organic 14C-label.
authors.affiliation: Department of Biology, Fisk University, Nashville, TN 37208.
authors.forename: H
authors.initials: H
authors.lastname: Spiller
authors.forename: W
authors.initials: W
authors.lastname: Stallings
mesh_terms: D001709:Biotechnology; D002245:Carbon Dioxide / Q000378:metabolism; D002250:Carbon Radioisotopes; D010944:Plants / Q000378:metabolism*; D013395:Sucrose / Q000378:metabolism; D014908:Triticum / Q000378:metabolism
chemical_list: D002250:Carbon Radioisotopes; D002245:Carbon Dioxide; D013395:Sucrose