Anti-T-cell receptor V beta 6 breaks tolerance in Mlsa mice and induces production of anti-Mlsa antibodies.
Tolerance to the minor lymphocyte stimulating (Mls) self-antigens has been shown to be due to the intrathymic deletion of T-cell clones bearing certain T-cell receptor (TcR) V beta regions. T cells bearing these V beta regions (V beta 6, V beta 7, V beta 8.1, V beta 9) are deleted in Mlsa-positive mice. This report demonstrates that hyperimmunization of AKR mice with anti-V beta 6 breaks tolerance to the self-antigen Mlsa and induces the development of anti-Mlsa antibodies. These antibodies had the capacity to block Mlsa-induced mixed lymphocyte responses between H-2 identical strain combinations and also between Mlsa-congeneic BALB.D2MA and parental BALB/c strains. Furthermore, when the responder lymphocytes were Mlsa-positive their response to allogeneic major or minor histocompatibility antigens was enhanced in the presence of this antiserum. This indicates that Mlsa may be present on T cells and that occupancy of this ligand leads to enhanced signal transduction.
Matossian-Rogers
A
A
Department of Immunology, Royal London Hospital Medical College, U.K.
de Giorgi
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L
D000818:Animals; D001323:Autoantibodies / Q000096:biosynthesis; D001402:B-Lymphocytes / Q000276:immunology; D004306:Dose-Response Relationship, Immunologic; D006183:H-2 Antigens / Q000276:immunology; D007108:Immune Tolerance / Q000276:immunology*; D007959:Lymphocyte Culture Test, Mixed; D051379:Mice; D008815:Mice, Inbred Strains; D016747:Minor Lymphocyte Stimulatory Antigens / Q000276:immunology*; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology
D001323:Autoantibodies; D006183:H-2 Antigens; D016747:Minor Lymphocyte Stimulatory Antigens; D016693:Receptors, Antigen, T-Cell, alpha-beta