Expression of the La Crosse M segment proteins in a recombinant vaccinia expression system mediates pH-dependent cellular fusion.
To study the expression of La Crosse virus (LAC) glycoproteins, G1 and G2, we constructed a cDNA copy of the open reading frame (ORF) of the middle RNA segment and expressed it in a recombinant vaccinia virus (VV.ORF). Cells infected with VV.ORF expressed G1 and G2 at the cell surface and formed syncytia with a pH profile similar to that of LAC. These experiments provide a system of studying the biological functions of the LAC glycoproteins, including processing, targeting, fusion, receptor binding, and antigenicity.
Jacoby
D R
DR
Department of Neurology, University of Pennsylvania Medical Center, Philadelphia 19104-6146.
Cooke
C
C
Prabakaran
I
I
Boland
J
J
Nathanson
N
N
Gonzalez-Scarano
F
F
D000818:Animals; D002459:Cell Fusion; D002460:Cell Line; D002462:Cell Membrane / Q000502:physiology; D003062:Codon / Q000235:genetics; D006224:Cricetinae; D002141:Encephalitis Virus, California / Q000235:genetics*; D005822:Genetic Vectors; D006863:Hydrogen-Ion Concentration; D007668:Kidney; D007700:Kinetics; D016366:Open Reading Frames; D012367:RNA, Viral / Q000235:genetics; D011995:Recombination, Genetic; D014616:Vaccinia virus / Q000235:genetics; D014764:Viral Proteins / Q000096:biosynthesis / Q000235:genetics*
D003062:Codon; D012367:RNA, Viral; D014764:Viral Proteins