Immunogenicity of various beta-lactam antibiotic-protein conjugates and cross-reactivity of the antibodies produced in guinea pig.
Immunogenic properties of bovine serum albumin (BSA) conjugates of various beta-lactam antibiotics (ABs) and cross-reactivity of the produced antibodies were investigated in guinea pigs. Each AB-BSA conjugate was immunized with complete Freund's adjuvant. Following the second injection, AB-specific antibodies were detected with high titers. Produced antibodies showed concentration-dependent inhibition by the corresponding AB. Cross-reactivity between the produced antibodies and ABs was evaluated with two methods, i.e. by testing for reactivity with the various AB-ovalbumin conjugates, and by assaying for inhibition of enzyme-linked immunosorbent assay (ELISA) by the various ABs. The latter method could detect minor cross-reactions more sensitively than the former one. The results suggested that similarity of the acyl side chain of a cephalosporin nucleus or a penicillin nucleus contributes to immunological cross-reactivity.
Katsutani
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Department of Drug Safety Research, Eisai Co., Gifu, Japan.
Shionoya
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H
D000818:Animals; D000900:Anti-Bacterial Agents / Q000276:immunology*; D000917:Antibody Formation; D001667:Binding, Competitive; D002511:Cephalosporins / Q000276:immunology*; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006168:Guinea Pigs; D012710:Serum Albumin, Bovine
D000900:Anti-Bacterial Agents; D002511:Cephalosporins; D012710:Serum Albumin, Bovine