title: Insulin-like growth factor (IGF)-I stimulates proliferation and migration of mouse ectoplacental cone cells, while IGF-II transforms them into trophoblastic giant cells in vitro.
abstract: We examined the effects of a 10.5-day placental extract and various growth factors (insulin-like growth factor [IGF]-1, IGF-II, epidermal growth factor [EGF], basic fibroblast growth factor [b-FGF], and transforming growth factor-alpha [TGF-alpha]) on proliferation, migration, and transformation of mouse ectoplacental cone (EPC) cells in vitro. Both the 10.5-day placental extract and IGF-I increased the surface area of the EPC cell colony and strongly stimulated the uptake of bromodeoxyuridine (Brd-U) as well as the migratory activity of EPC cells on the plastic culture dish. Such effects of 10.5-day placental extract were partly inhibited by the addiction of anti-IGF-I antibody. On the other hand, IGF-II did not significantly affect proliferation and migration of EPC cells, but increased the number of cells having a large nucleus (trophoblastic giant cells; TGCs) per EPC and enlarged the ploidy levels of EPC cells. Histochemical staining with succinyl wheat germ agglutinin (s-WGA), an in situ marker for secondary TGCs on Day 10.5 post coitum revealed that IGF-II or the placental extract induced the expression of s-WGA-binding glycoproteins in the TGCs in vitro. The effects of IGF-II or placental extracts were also inhibited by anti-IGF-II monoclonal antibody. No appreciable effect was found in the EPCs cultured with TGF-alpha, whereas b-FGF and EGF promoted migratory activity of the cells. The present study indicates that IGFs or IGF-like substances may be present in the mouse placenta, that IGF-I may promote the proliferation and migration of EPC cells, and that IGF-II may induce the transformation of EPC cells into TGCs in vitro.
authors.affiliation: Department of Veterinary Anatomy, Faculty of Agriculture, University of Tokyo, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Kanai-Azuma
authors.forename: Y
authors.initials: Y
authors.lastname: Kanai
authors.forename: M
authors.initials: M
authors.lastname: Kurohmaru
authors.forename: S
authors.initials: S
authors.lastname: Sakai
authors.forename: Y
authors.initials: Y
authors.lastname: Hayashi
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects*; D002465:Cell Movement / Q000187:drug effects*; D002478:Cells, Cultured; D004261:DNA Replication / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004815:Epidermal Growth Factor / Q000494:pharmacology; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D007335:Insulin-Like Growth Factor II / Q000494:pharmacology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008858:Microscopy, Phase-Contrast; D010920:Placenta; D014020:Tissue Extracts / Q000494:pharmacology; D016211:Transforming Growth Factor alpha / Q000494:pharmacology; D014327:Trophoblasts / Q000502:physiology*
chemical_list: D014020:Tissue Extracts; D016211:Transforming Growth Factor alpha; D016222:Fibroblast Growth Factor 2; D004815:Epidermal Growth Factor; D007335:Insulin-Like Growth Factor II