Generation of somatic cell hybrids capable of proliferating and secreting human monoclonal antibody without any growth factor supplements.
A novel cell line called Trioma, which can proliferate and secrete a human monoclonal antibody in the basal medium, was established. Trioma was generated by somatic cell hybridization with human x human hybridoma and A431c, which is a cell line able to grow autonomously in the basal medium. A Trioma called TriH8 has been kept growing in the DMEM/F-12 medium for over 1 year and stably producing stomach cancer-reactive human IgM into culture medium at 10 micrograms/ml. TriH8 has a characteristic cytological phenotype, that is, to diminish cell growth in the presence of epidermal growth factor.
Hagiwara
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Hagiwara Institute of Health, Hyogo, Japan.
Yuasa
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D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology; D000912:Antibodies, Neoplasm / Q000096:biosynthesis* / Q000276:immunology; D002455:Cell Division; D002460:Cell Line; D003363:Cost Control; D016895:Culture Media, Serum-Free / Q000494:pharmacology*; D046508:Culture Techniques / Q000191:economics; D004247:DNA / Q000032:analysis; D004815:Epidermal Growth Factor / Q000494:pharmacology; D006801:Humans; D006822:Hybrid Cells / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D006825:Hybridomas / Q000276:immunology; D007075:Immunoglobulin M / Q000096:biosynthesis / Q000276:immunology; D013274:Stomach Neoplasms / Q000276:immunology
D000911:Antibodies, Monoclonal; D000912:Antibodies, Neoplasm; D016895:Culture Media, Serum-Free; D007075:Immunoglobulin M; D004815:Epidermal Growth Factor; D004247:DNA