Rainbow trout ovarian cholesterol side-chain cleavage cytochrome P450 (P450scc). cDNA cloning and mRNA expression during oogenesis.
A cDNA clone encoding cholesterol side-chain cleavage cytochrome P450 (P450scc) was isolated from a rainbow trout ovarian follicle cDNA library. The cDNA contains an open reading frame of 1,542 nucleotides encoding a protein of 514 amino acids. The predicted amino acid sequence of trout P450scc shows 48% homology with that of human, and 46% homology with that of rat, bovine and pig. P450scc activity was confirmed by transfected COS-1 monkey kidney tumour cells with an expression vector for trout P450scc cDNA and subsequent detection of conversion from 25-hydroxycholesterol to pregnenolone by radioimmunoassay. The cDNA only hybridized to a single 1.8 kb RNA transcript. The transcript was not found in early vitellogenic follicles, barely detected in postvitellogenic follicles, and abundant in postovulatory follicles.
Takahashi
M
M
Department of Molecular Biomechanics, Graduate University for Advanced Studies, Okazaki, Japan.
Tanaka
M
M
Sakai
N
N
Adachi
S
S
Miller
W L
WL
Nagahama
Y
Y
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002786:Cholesterol Side-Chain Cleavage Enzyme / Q000235:genetics*; D003001:Cloning, Molecular; D003062:Codon; D004247:DNA / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D005260:Female; D015870:Gene Expression; D006801:Humans; D008969:Molecular Sequence Data; D009866:Oogenesis / Q000235:genetics*; D010053:Ovary / Q000201:enzymology*; D012333:RNA, Messenger / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D014162:Transfection; D014337:Trout / Q000235:genetics*
D003062:Codon; D012333:RNA, Messenger; D004247:DNA; D002786:Cholesterol Side-Chain Cleavage Enzyme