Ki-67 expression and BrdUrd incorporation as markers of proliferative activity in human prostate tumour models.
The validity of the use of the monoclonal antibodies Ki-67 and anti-BrdUrd to evaluate proliferative activity of human prostate tumour models was studied. Growth of the transplantable PC-82 and PC-EW prostate tumours, as assessed by tumour volume measurements, was significantly correlated with the proliferative activity as reflected by BrdUrd incorporation into DNA (r = 0.64 and r = 0.78, respectively). The proliferative activity of PC-82 tumours detected by Ki-67 antigen expression paralleled the pattern observed with BrdUrd (r = 0.51) and a significant correlation (r = 0.60) between the results obtained with both markers was found. In growing PC-82 and PC-EW tumours only small variations in the Ki-67 and BrdUrd indices were observed. In contrast, Ki-67 expression in regressing PC-82 tumours varied considerably (2.7 +/- 2.2%). The BrdUrd index in regressing PC-82 tumours showed less variation (1.3 +/- 0.2%), but part of the BrdUrd-positive cells were found in the stromal (murine) part of the regressing tissue. It is concluded that the Ki-67 and BrdUrd proliferation markers are reliable parameters to monitor changes in growth of prostate tumour lines, but that in slow growing or regressing tumours Ki-67 and BrdUrd data should be interpreted with caution.
van Weerden
W M
WM
Department of Urology, Erasmus University, Rotterdam, The Netherlands.
Moerings
E P
EP
van Kreuningen
A
A
de Jong
F H
FH
van Steenbrugge
G J
GJ
Schröder
F H
FH
D000818:Animals; D014408:Biomarkers, Tumor / Q000032:analysis*; D001973:Bromodeoxyuridine / Q000378:metabolism*; D002455:Cell Division; D013196:Dihydrotestosterone / Q000032:analysis; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D019394:Ki-67 Antigen; D008297:Male; D051379:Mice; D008954:Models, Biological; D009363:Neoplasm Proteins / Q000096:biosynthesis*; D009687:Nuclear Proteins / Q000096:biosynthesis*; D011471:Prostatic Neoplasms / Q000737:chemistry / Q000378:metabolism / Q000473:pathology*; D013739:Testosterone / Q000032:analysis / Q000097:blood; D014407:Tumor Cells, Cultured / Q000637:transplantation
D014408:Biomarkers, Tumor; D019394:Ki-67 Antigen; D009363:Neoplasm Proteins; D009687:Nuclear Proteins; D013196:Dihydrotestosterone; D013739:Testosterone; D001973:Bromodeoxyuridine