Purification and characterization of an N-acetyllactosamine-specific lectin from larvae of a moth, Phalera flavescens.
A lectin (Phalera flavescens agglutinin, PFA) of a moth (P. flavescens) has been isolated from hemolymph by DEAE-Toyopearl followed by Cellulofine GCL-1000 chromatography. On size-exclusion chromatography, PFA showed a molecular mass of about 74 kDa, and on sodium dodecyl sulfate-polyacrylamide gel electrophoresis it was separated into two different subunits, glycosylated 18-kDa and unglycosylated 17-kDa subunits, suggesting that PFA occurs as a heterotetrameric protein. N-Acetyllactosamine and laminin inhibited the hemagglutinating activity of PFA. Studies involving Synsorbs showed that PFA interacts with the Gal beta 1-->4GlcNAc group and that nonsubstituted hydroxyl groups at the C-2 and C-4 positions of the galactose residue were essential for interaction with PFA, whereas substitution at the C-3 position of the galactose did not inhibit the interaction between PFA and sugar chains associated with N-acetyllactosamine. PFA is not mitogenic for native or desialylated mouse thymocytes or splenocytes.
Umetsu
K
K
Department of Forensic Medicine, Yamagata University School of Medicine, Japan.
Yamashita
K
K
Suzuki
J
J
Yamashita
T
T
Suzuki
T
T
D000373:Agglutinins / Q000097:blood* / Q000737:chemistry; D000595:Amino Acid Sequence; D000606:Amino Sugars / Q000378:metabolism* / Q000494:pharmacology; D000818:Animals; D002240:Carbohydrate Sequence; D002845:Chromatography; D004591:Electrophoresis, Polyacrylamide Gel; D006031:Glycosylation; D006384:Hemagglutination; D006458:Hemolymph / Q000737:chemistry*; D006801:Humans; D007797:Laminin / Q000494:pharmacology; D007814:Larva / Q000737:chemistry; D037102:Lectins; D046911:Macromolecular Substances; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009036:Moths; D011817:Rabbits
D000373:Agglutinins; D000606:Amino Sugars; D007797:Laminin; D037102:Lectins; D046911:Macromolecular Substances; C079920:Phalera flavescens agglutinin; C000458:N-acetyllactosamine