title: Role of histocompatibility antigen gene and protooncogene expressions in intracerebral tumorigenicity of mouse neuroblastoma.
abstract: The role of N-myc, c-src, and major histocompatibility complex (MHC, H-2 in the mouse) class I antigen gene expressions in dimethyl sulfoxide (DMSO)-induced differentiation and intracerebral tumorigenicity was examined using a mouse MNB85 neuroblastoma cell line. A fluorescence-activated cell sorter disclosed cell-surface MHC enhancement by DMSO, causing an increase in cytotoxic T-lymphocyte sensitivity. Southern blot analysis verified a single copy of the proto-oncogenes and MHC deoxyribonucleic acids in both untreated and DMSO-treated MNB85 cells. Northern blot analysis indicated that DMSO treatment induced a decrease in N-myc and an increase in c-src and MHC messenger ribonucleic acids. Nuclear run-off transcription assay revealed down-regulation of N-myc at a posttranscriptional level, contrasted with primary up-regulation of c-src at a transcriptional level. Immunoprecipitation after treatment with enzyme endo-beta-N-acetyl-glycoseamidase H proved that the terminal glycosylation of MHC heavy-chain gene products normally occurs in the Golgi apparatus of MNB85 cells. Intracerebral tumorigenicity assay showed that cells highly MHC-expressed by DMSO were less tumorigenic than untreated cells in association with DMSO-augmented cytotoxic T-lymphocyte susceptibility. These results suggest that proto-oncogenes may be linked to cellular differentiation, while cell-surface MHC gene expression influences intracerebral immunosurveillance.
authors.affiliation: Department of Neurosurgery, Shimane Medical University, Izumo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Yamasaki
authors.forename: G
authors.initials: G
authors.lastname: Klein
authors.forename: H G
authors.initials: HG
authors.lastname: Ljunggren
authors.forename: K
authors.initials: K
authors.lastname: Kärre
authors.forename: K
authors.initials: K
authors.lastname: Moritake
authors.forename: J T
authors.initials: JT
authors.lastname: Paine
authors.forename: H
authors.initials: H
authors.lastname: Kikuchi
mesh_terms: D000818:Animals; D001932:Brain Neoplasms / Q000235:genetics*; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D005805:Genes, MHC Class I / Q000235:genetics*; D016259:Genes, myc / Q000235:genetics; D016391:Genes, src / Q000235:genetics; D051379:Mice; D008815:Mice, Inbred Strains; D009447:Neuroblastoma / Q000235:genetics*; D011519:Proto-Oncogenes / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D012334:RNA, Neoplasm / Q000032:analysis; D014407:Tumor Cells, Cultured
chemical_list: D012333:RNA, Messenger; D012334:RNA, Neoplasm; D004121:Dimethyl Sulfoxide