Effects of all-trans-retinoic acid on melanocyte adhesion and motility.
Human epidermal melanocytes were treated with all-trans-retinoic acid (RA) and examined for adhesion to bovine serum albumin-, fibronectin- and laminin-coated culture dishes. Control and treated cells were also examined for motility into micropore filters coated with the same proteins. Treatment of the cells with 3 x 10(-6) M RA for 3-4 days resulted in inhibition of attachment to all three substrates. Decreased attachment was observed within 1.5 h. Inhibition of attachment was not due to toxicity because differences between control and treated cells disappeared by 18 h, when most of the cells (approximately 75%) were attached and spread on all three substrates. The same treatment that inhibited adhesion also reduced migration into the interstices of micropore filters coated with the same three proteins. In additional experiments, human and mouse melanoma cell lines were examined in place of normal melanocytes. RA treatment also blocked adhesion and motility of these cells. The malignant melanoma cells were less sensitive to RA than normal melanocytes in the adhesion assay but were equally sensitive in the motility assay. The ability of RA to inhibit melanocyte adhesion and motility as well as melanocyte growth could explain, in part, the capacity of retinoids to modulate melanocyte function in hyperpigmented skin lesions.
Situ
R
R
Department of Pathology, University of Michigan Medical School, Ann Arbor 48109.
Inman
D R
DR
Fligiel
S E
SE
Varani
J
J
D000818:Animals; D002417:Cattle; D002448:Cell Adhesion / Q000187:drug effects; D002461:Cell Line, Transformed; D002465:Cell Movement / Q000187:drug effects; D005353:Fibronectins; D006801:Humans; D007231:Infant, Newborn; D007797:Laminin; D008544:Melanocytes / Q000187:drug effects* / Q000502:physiology; D008545:Melanoma / Q000473:pathology / Q000503:physiopathology; D012709:Serum Albumin; D012878:Skin Neoplasms / Q000473:pathology / Q000503:physiopathology; D014212:Tretinoin / Q000494:pharmacology*
D005353:Fibronectins; D007797:Laminin; D012709:Serum Albumin; D014212:Tretinoin