Immunonephelometry and radial immunodiffusion compared for measuring serum retinol-binding protein.
We compared a nephelometric method and a radial immunodiffusion (RID) assay for the measurement of retinol-binding protein in samples of serum from children with malignancies. The mean (+/- standard deviation) retinol-binding protein concentration as measured by the Behring Nephelometer was 31.0 +/- 15.6 mg/l; the mean by RID was 31.2 +/- 15.7 mg/l. This difference was not statistically significant by Student's t test (P = 0.6), and the correlation coefficient (r) was 0.87. Thus, the Behring Nephelometer method measures retinol-binding protein rapidly and as accurately as radial immunodiffusion.
Malvy
D J
DJ
INSERM U056, Hôpital de Bicêtre, France.
Povéda
J D
JD
Debruyne
M
M
Burtschy
B
B
Dostalova
L
L
Amédée-Manesme
O
O
D016543:Central Nervous System Neoplasms / Q000097:blood; D002648:Child; D003627:Data Interpretation, Statistical; D006801:Humans; D005779:Immunodiffusion; D007938:Leukemia / Q000097:blood; D008223:Lymphoma / Q000097:blood / Q000503:physiopathology; D009369:Neoplasms / Q000097:blood*; D009391:Nephelometry and Turbidimetry; D012177:Retinol-Binding Proteins / Q000032:analysis*
D012177:Retinol-Binding Proteins