Recombinant enterotoxins as vaccines against Escherichia coli-mediated diarrhoea.
A fusion protein, comprising the B subunit of the heat-labile enterotoxin and a portion of the precursor to the heat-stable enterotoxin of Escherichia coli, has been created by recombinant genetic techniques. It is exported successfully to the bacterial periplasm and assembles into pentamers which retain the ability to bind to GM1 ganglioside. Native toxin epitopes are displayed and the molecule can be easily purified from periplasmic extracts of cells expressing the gene fusion. Although the protein carries the natural sequence of the heat-stable enterotoxin, it is greatly attenuated in toxicity. Systemic immunization of mice or oral administration of the fusion elicits antibody responses against both classes of E. coli enterotoxin.
Aitken
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Department of Microbiology, University of Glasgow, UK.
Hirst
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TR
D000284:Administration, Oral; D000595:Amino Acid Sequence; D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis; D001427:Bacterial Toxins / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology* / Q000378:metabolism / Q000633:toxicity; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000276:immunology / Q000633:toxicity; D001483:Base Sequence; D003967:Diarrhea / Q000382:microbiology / Q000517:prevention & control*; D004768:Enterotoxins / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology* / Q000378:metabolism / Q000633:toxicity; D004927:Escherichia coli Infections / Q000517:prevention & control*; D029968:Escherichia coli Proteins; D022361:Escherichia coli Vaccines; D005260:Female; D005677:G(M1) Ganglioside / Q000378:metabolism; D007279:Injections, Subcutaneous; D051379:Mice; D008969:Molecular Sequence Data; D011498:Protein Precursors / Q000008:administration & dosage / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000276:immunology* / Q000378:metabolism / Q000633:toxicity; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000276:immunology / Q000633:toxicity
D000907:Antibodies, Bacterial; D001427:Bacterial Toxins; D001428:Bacterial Vaccines; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D022361:Escherichia coli Vaccines; D011498:Protein Precursors; D011993:Recombinant Fusion Proteins; D014614:Vaccines, Synthetic; C034151:heat stable toxin (E coli); D005677:G(M1) Ganglioside; C027538:heat-labile enterotoxin, E coli