Species difference in hydroperoxide-scavenging enzymes with special reference to glutathione peroxidase in guinea-pigs.
1. As guinea-pigs have been reported to have a markedly low activity of glutathione peroxidase (GSH-Px), the activity of other hydroperoxide-scavenging enzymes was investigated. 2. Catalase activity in guinea-pig tissues was 2-3 times higher than that of mice or rats. 3. Approximately 90% of catalase activity was found in the soluble fraction of guinea-pig liver, suggesting a compensatory role of catalase in removing H2O2 in the cytosol of guinea-pig tissues. 4. In erythrocytes, GSH-Px activity does not differ among rodents. This may reflect the fact that GSH-Px is the sole enzyme in the removal of organic hydroperoxides in erythrocytes where glutathione S-transferase activity is barely detectable.
Himeno
S
S
Kitasato University, School of Pharmaceutical Sciences, Tokyo, Japan.
Takekawa
A
A
Imura
N
N
D000818:Animals; D002374:Catalase / Q000378:metabolism; D003600:Cytosol / Q000201:enzymology; D004912:Erythrocytes / Q000201:enzymology; D016166:Free Radical Scavengers; D005979:Glutathione Peroxidase / Q000097:blood / Q000378:metabolism*; D006168:Guinea Pigs; D006861:Hydrogen Peroxide / Q000378:metabolism*; D007668:Kidney / Q000201:enzymology; D008099:Liver / Q000201:enzymology / Q000648:ultrastructure; D008168:Lung / Q000201:enzymology; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009206:Myocardium / Q000201:enzymology; D051381:Rats; D017208:Rats, Wistar; D013045:Species Specificity
D016166:Free Radical Scavengers; D006861:Hydrogen Peroxide; D002374:Catalase; D005979:Glutathione Peroxidase