The barley alpha-thionin promoter is rich in negative regulatory motifs and directs tissue-specific expression of a reporter gene in tobacco.
The promoter of the barley alpha-thionin gene (1.6 kb) fused to the beta-glucuronidase (GUS) gene directs temporally-controlled, tissue-specific expression in the endosperm of transgenic tobacco. The nucleotide sequence of this promoter shows negative regulatory motifs which have been functionally analyzed in other genes.
Fernández
J A
JA
Laboratorio de Bioquímica y Biología Molecular, E.T.S. Ingenieros Agrónomos-UPM, Madrid, Spain.
Moreno
M
M
Carmona
M J
MJ
Castagnaro
A
A
García-Olmedo
F
F
D023181:Antimicrobial Cationic Peptides; D001483:Base Sequence; D005966:Glucuronidase / Q000235:genetics; D001467:Hordeum / Q000235:genetics*; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010940:Plant Proteins / Q000235:genetics*; D030821:Plants, Genetically Modified; D010947:Plants, Toxic; D011401:Promoter Regions, Genetic; D012045:Regulatory Sequences, Nucleic Acid; D014026:Nicotiana / Q000235:genetics
D023181:Antimicrobial Cationic Peptides; D010940:Plant Proteins; D005966:Glucuronidase