Effect of the composition of vitrification media on survival of rabbit embryos.
A total of 448 rabbit morula stage embryos were used to examine the effect of modified vitrification procedure and vitrification media containing 10% (1.36 M) glycerol, 20% (2.72 M) 1,2-propanediol, and 1 M sucrose. The best result, 89.5% embryos developed in vitro, was obtained after equilibration, vitrification, and dilution of embryos using media in which the salt concentration was maintained constant on a molar basis. Media prepared on a molal basis were more toxic and/or less efficient.
Papis
K
K
National Institute of Animal Industry, Tsukuba Norindanchi, Ibaraki, Japan.
Fujikawa
S
S
Kojima
T
T
Oguri
N
N
D000818:Animals; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents; D004622:Embryo, Mammalian; D005069:Evaluation Studies as Topic; D005990:Glycerol; D009028:Morula; D019946:Propylene Glycol; D011409:Propylene Glycols; D011817:Rabbits; D012996:Solutions; D013395:Sucrose
D003451:Cryoprotective Agents; D011409:Propylene Glycols; D012996:Solutions; D013395:Sucrose; D019946:Propylene Glycol; D005990:Glycerol