title: Effect of group B streptococcal sepsis on diaphragmatic function in young piglets.
abstract: Recent studies indicate that diaphragmatic pressure generation (Pdi) is impaired by bacterial infection. However, group B streptococcus (GBS) had no effect on Pdi when infused into 4-wk-old piglets. As responsiveness to GBS is age-dependent, we therefore studied the acute effect of GBS infusion on Pdi, using a younger, 2-wk-old piglet model. Using trans-Pdi with phrenic nerve stimulation, we studied the effect of continuous GBS infusion in seven anesthetized, spontaneously breathing 2-wk-old piglets. Pdi was measured under baseline conditions (50% O2/50% N2) and at 1, 2, and 4 h of GBS infusion. GBS was infused at a rate which caused a doubling of the pulmonary artery pressure but which avoided hypotension or acidosis--both of which can decrease Pdi. In addition, the piglets were kept hyperoxic [PaO2 > 13.3 kPa (100 torr)], and no piglet with hypercapnia [PaCO2 > 8.7 kPa (65 torr)] was included, as hypoxia and hypercapnia can also cause respiratory muscle dysfunction. For the GBS group, diaphragmatic contractility declined significantly by 1 h at 30-, 50-, and 100-Hz stimulation frequency and, by 2 h, was significantly decreased at all frequencies. We conclude that 2-wk-old piglets, in contrast to 4-wk-old piglets, demonstrate a decline in Pdi during GBS infusion. These data demonstrate an age-related response to GBS in the piglet.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Pediatrics, University of Washington School of Medicine, Seattle 98195.
authors.forename: T D
authors.initials: TD
authors.lastname: Murphy
authors.forename: R L
authors.initials: RL
authors.lastname: Gibson
authors.forename: T A
authors.initials: TA
authors.lastname: Standaert
authors.forename: D E
authors.initials: DE
authors.lastname: Mayock
authors.forename: D E
authors.initials: DE
authors.lastname: Woodrum
mesh_terms: D000818:Animals; D003964:Diaphragm / Q000098:blood supply / Q000503:physiopathology*; D006439:Hemodynamics / Q000502:physiology; D009119:Muscle Contraction / Q000502:physiology; D011312:Pressure; D011659:Pulmonary Gas Exchange / Q000502:physiology; D015656:Respiratory Mechanics / Q000502:physiology; D013290:Streptococcal Infections / Q000503:physiopathology*; D013292:Streptococcus agalactiae; D013552:Swine