Characterization of the alternative oxidase protein in the yeast Hansenula anomala.
The cyanide-resistant respiratory pathway is induced by respiratory inhibitors in the yeast Hansenula anomala. A monoclonal antibody against the alternative oxidase in the higher plant Sauromatum guttatum cross-reacted with a 36-kDa mitochondrial protein induced by antimycin A in H. anomala and with a protein encoded by a cDNA which was previously cloned for an antimycin A-inducible mRNA in the yeast. There was a similarity in the amino acid sequence between the cDNA-encoded protein and the plant alternative oxidase protein. We propose that the 36-kDa mitochondrial protein encoded by the cDNA is a component of alternative oxidase in H. anomala.
Sakajo
S
S
Department of Biochemistry, Niigata College of Pharmacy, Japan.
Minagawa
N
N
Yoshimoto
A
A
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal; D000968:Antimycin A / Q000494:pharmacology; D015153:Blotting, Western; D004247:DNA / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D010843:Pichia / Q000201:enzymology*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000032:analysis; D011994:Recombinant Proteins / Q000032:analysis; D017386:Sequence Homology, Amino Acid; D001616:beta-Galactosidase / Q000235:genetics
D000911:Antibodies, Monoclonal; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; D000968:Antimycin A; D004247:DNA; D010088:Oxidoreductases; C079923:alternative oxidase, Hansenula anomala; D001616:beta-Galactosidase