Banding patterns of Fasciola hepatica and Fasciola gigantica (Trematoda) by isoelectric focusing.
Liver flukes Fasciola hepatica and Fasciola gigantica are polymorphic and vary morphologically depending upon the host being parasitized. It is known also that mixed infection occurs where both species are present. A technique involving protein separation was used to distinguish the 2 species. Isoelectric focusing of soluble proteins was performed on polyacrylamide gels using whole-body proteins from adult flukes. Although many bands appeared common to both species and some were shared with host tissues, the banding patterns could be used to distinguish 1 species from the other. Soluble protein isoelectric focusing is simple, reproducible, and has very good resolution. It seems well suited to the differentiation of the 2 fluke species.
Lee
C G
CG
College of Veterinary Medicine, Oregon State University, Corvallis 97331.
Zimmerman
G L
GL
D000818:Animals; D002417:Cattle; D005209:Fasciola / Q000737:chemistry*; D005210:Fasciola hepatica / Q000737:chemistry*; D015801:Helminth Proteins / Q000032:analysis*; D007525:Isoelectric Focusing; D015203:Reproducibility of Results
D015801:Helminth Proteins