Principles of selective inactivation of viral genome. VIII. The influence of beta-propiolactone on immunogenic and protective activities of influenza virus.
The influence of beta-propiolactone action on the immunogenic and protective activity of the influenza virus A/WSN/33 (H1N1) has been studied. The production of antibodies against virion surface antigens in mice immunized intramuscularly by the modified virus was enhanced with the increase of inoculating dose from 6 x 10(7) to 1.5 x 10(8) viral particles per animal. The immunizing dose of 6 x 10(7) produced complete protection of immunized animals against a lethal challenge of A/WSN/33 virus. The inhibition of virus reproduction in animal lungs was increased with the increase of the virus immunizing dose up to 6 x 10(8). At a constant dose the inhibition of virus reproduction decreases with the increase of the virus modification extent.
Budowsky
E I
EI
N.D. Zelinsky Institute of Organic Chemistry, USSR Academy of Sciences, Moscow.
Smirnov YuA
Shenderovich
S F
SF
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D004306:Dose-Response Relationship, Immunologic; D016679:Genome, Viral; D009980:Influenza A virus / Q000187:drug effects / Q000235:genetics / Q000276:immunology*; D007252:Influenza Vaccines / Q000008:administration & dosage / Q000276:immunology / Q000302:isolation & purification*; D051379:Mice; D008807:Mice, Inbred BALB C; D009976:Orthomyxoviridae Infections / Q000517:prevention & control; D011420:Propiolactone / Q000494:pharmacology*; D015164:Vaccines, Inactivated / Q000008:administration & dosage / Q000276:immunology / Q000302:isolation & purification; D014779:Virus Replication
D000914:Antibodies, Viral; D007252:Influenza Vaccines; D015164:Vaccines, Inactivated; D011420:Propiolactone