title: Conversion of a trans-spliced C. elegans gene into a conventional gene by introduction of a splice donor site.
abstract: In Caenorhabditis elegans, pre-mRNAs that are trans-spliced are distinguished by the presence of an 'outron', intron-like RNA at the 5' end followed by a splice acceptor. We report that trans-splicing of the rol-6 gene can be completely suppressed simply by introducing a donor site into its 173 nt outron, at a site 50 nt upstream of the trans-splice site, thereby converting rol-6 into a conventional gene with a spliced intron near its 5' end. When the consensus donor site was inserted at sites further upstream it was less effective in replacing transplicing with cis-splicing. Surprisingly, the length of the intron was not the important variable, since lengthening of the 50 nt intron to 250 nt did not restore trans-splicing. Apparently the context into which the splice site was introduced determined the efficiency of its use. These results support the conclusion that the sole signal for trans-splicing is the presence of an outron. Clearly, cis- and trans-splice acceptor sites are interchangeable, allowing the possibility of competition between the two types of splicing.
authors.affiliation: Department of Biology, Indiana University, Bloomington 47405.
authors.forename: R
authors.initials: R
authors.lastname: Conrad
authors.forename: R F
authors.initials: RF
authors.lastname: Liou
authors.forename: T
authors.initials: T
authors.lastname: Blumenthal
mesh_terms: D000818:Animals; D001483:Base Sequence; D017173:Caenorhabditis elegans / Q000235:genetics*; D003094:Collagen / Q000235:genetics; D004247:DNA; D005091:Exons; D017238:Genes, Helminth; D007438:Introns; D008969:Molecular Sequence Data; D012326:RNA Splicing; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D003094:Collagen; D004247:DNA