Glycosphingolipid patterns in human promyelocytic HL-60 leukemia cells susceptible or resistant to differentiation induction by phorbol 12-myristate 13-acetate.
The patterns of glycosphingolipids (GSLs) were analyzed in an HL-60 cell variant, HL-205, which is susceptible to phorbol 12-myristate 13-acetate (PMA)-induced monocyte/macrophage differentiation, and in an HL-60 cell variant, HL-525, which is resistant to such differentiation. The amounts and types of the GSLs were similar in both the HL-205 and HL-525 cells and they resemble those of granulocytes. Treatment with 3 nM PMA caused the susceptible HL-205 cells (but not the resistant cells) to acquire a new GSL pattern which resembles that of monocytes. This new pattern was characterized by increases in the level of a neutral GSL, Gb3Cer, from trace levels to 0.05 mg/10(9) cells and of an acidic GSL, GM3 ganglioside, from 0.03 to 0.33 mg/10(9) cells. The increases in the level of this ganglioside were found to be due to an increase in CMP-N-acetylneuraminic acid:lactosylceramide sialyltransferase activity. These results indicate an association between PMA-induced terminal differentiation along the monocyte/macrophage cell lineage and PMA-evoked increases in specific GSLs, GM3 in particular, which is due to a rise in the activity of its synthetic enzyme.
Kiguchi
K
K
Biological and Medical Research Division, Argonne National Laboratory, Illinois 60439.
Henning-Chubb
C
C
Huberman
E
E
D002240:Carbohydrate Sequence; D002454:Cell Differentiation / Q000187:drug effects; D004790:Enzyme Induction / Q000187:drug effects; D005679:G(M3) Ganglioside / Q000096:biosynthesis; D006028:Glycosphingolipids / Q000096:biosynthesis* / Q000737:chemistry; D006801:Humans; D015473:Leukemia, Promyelocytic, Acute / Q000235:genetics; D008969:Molecular Sequence Data; D009000:Monocytes / Q000378:metabolism; D012799:Sialyltransferases / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000187:drug effects*; D000094802:beta-D-Galactoside alpha 2-6-Sialyltransferase
D005679:G(M3) Ganglioside; D006028:Glycosphingolipids; D012799:Sialyltransferases; D013755:Tetradecanoylphorbol Acetate; D000094802:beta-D-Galactoside alpha 2-6-Sialyltransferase