A method for obtaining high-quality sequences from the non-biotinylated, free ssDNA remaining after solid-phase sequencing.
Lee
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YH
Marine Biology Research Division, Scripps Institution of Oceanography, University of California, San Diego, La Jolla 92093.
Vacquier
V D
VD
D001426:Bacterial Proteins; D001710:Biotin; D004277:DNA, Single-Stranded / Q000737:chemistry*; D004121:Dimethyl Sulfoxide; D004229:Dithiothreitol; D008280:Magnetics; D009691:Nucleic Acid Denaturation; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA / Q000379:methods*; D012972:Sodium Hydroxide; D019809:Streptavidin
D001426:Bacterial Proteins; D004277:DNA, Single-Stranded; D012972:Sodium Hydroxide; D001710:Biotin; D019809:Streptavidin; D004229:Dithiothreitol; D004121:Dimethyl Sulfoxide