Effects of transforming growth factor-beta on collagen gene expression and collagen synthesis level in mineralizing cultures of osteoblast-like cell line, MC3T3-E1.
1. High levels of type I collagen mRNA and [3H]proline incorporation into collagenase digestable protein by MC3T3-E1 cells were detected during the first 7 days of culture, after which they declined. 2. Type I collagen gene expression was stimulated by TGF-beta in the early culture stage when the collagen gene expression was fully functioning. 3. However, these stimulatory effects disappeared at the differentiation stages. Although collagen gene expression was stimulated by TGF-beta (2.0 ng/ml) in early culture, collagen synthesis in medium was not. 4. This study shows that collagen synthesis and collagen gene expression were affected by the state of differentiation in MC3T3-E1 cells and that the rate of stimulation by TGF-beta in collagen gene expression decreased over time in culture.
Shibata
Y
Y
Department of Biochemistry, Nihon University School of Dentistry, Matsudo, Chiba, Japan.
Abiko
Y
Y
Moriya
Y
Y
Yoshida
W
W
Takiguchi
H
H
D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D003094:Collagen / Q000096:biosynthesis* / Q000235:genetics*; D015870:Gene Expression; D051379:Mice; D008903:Minerals / Q000378:metabolism*; D010006:Osteoblasts / Q000378:metabolism*; D011392:Proline / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000494:pharmacology*
D008903:Minerals; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta; D003094:Collagen; D011392:Proline