Stimulation of glucose incorporation into glycogen by E-series prostaglandins in cultured rat hepatocytes.
In primary cultures of rat hepatocytes, 16,16-dimethylprostaglandin E2 (16,16-dimethyl PGE2), a biologically active analogue of prostaglandin E2 (PGE2), stimulated the basal rate of [14C]glucose incorporation into glycogen. 16,16-Dimethyl PGE2 caused concentration-dependent stimulation (ED50: 10(-8) M) with a maximum 2-3 h after its addition. Prostaglandin E1 (PGE1), PGE2 and prostaglandin F2 alpha (PGF2 alpha) stimulated also the incorporation, but less effectively than 16,16-dimethyl PGE2. However, prostaglandin D2 (PGD2) did not show such effect. Cellular glycogen analysis revealed that PGE2 and 16,16-dimethyl PGE2 increased a net glycogen accumulation time-dependently. Pretreatment of the cultured hepatocytes with pertussis toxin blocked the effects of PGE2 and 16,16-dimethyl PGE2 completely and concentration-dependently. These findings indicate that E-series prostaglandins have significant effects on hepatic glycogenesis via pertussis-toxin-sensitive G protein, in addition to their inhibitory effects on hormone-stimulated glycogenolysis reported previously (Okumura, T., Sago, T. and Saito, K. (1988) Prostaglandins 36, 463-475).
Okumura
T
T
Department of Medical Chemistry, Kansai Medical University, Osaka, Japan.
Kanemaki
T
T
Kitade
H
H
D015064:16,16-Dimethylprostaglandin E2 / Q000037:antagonists & inhibitors / Q000494:pharmacology; D000527:Alprostadil / Q000494:pharmacology; D000818:Animals; D002250:Carbon Radioisotopes; D002478:Cells, Cultured / Q000187:drug effects; D015232:Dinoprostone / Q000037:antagonists & inhibitors / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D019204:GTP-Binding Proteins / Q000378:metabolism; D005947:Glucose / Q000378:metabolism*; D006003:Glycogen / Q000096:biosynthesis*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D037342:Pertussis Toxin; D011458:Prostaglandins E / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D010566:Virulence Factors, Bordetella / Q000494:pharmacology
D002250:Carbon Radioisotopes; D011458:Prostaglandins E; D010566:Virulence Factors, Bordetella; D006003:Glycogen; D037342:Pertussis Toxin; D019204:GTP-Binding Proteins; D000527:Alprostadil; D005947:Glucose; D015232:Dinoprostone; D015064:16,16-Dimethylprostaglandin E2