Involvement of an activation protein in the methanol:2-mercaptoethanesulfonic acid methyltransferase reaction in Methanosarcina barkeri.
Methanol:5-hydroxybenzimidazolylcobamide methyltransferase (MT1) is the first of two enzymes required for transfer of the methyl group of methanol to 2-mercaptoethanesulfonic acid in Methanosarcina barkeri. MT1 binds the methyl group of methanol to its corrinoid prosthetic group only when the central cobalt atom of the corrinoid is present in the highly reduced Co(I) state. However, upon manipulation of MT1 and even during catalysis, the enzyme becomes inactivated as the result of Co(I) oxidation. Reactivation requires H2, hydrogenase, and ATP. Ferredoxin stimulated the apparent reaction rate of methyl group transfer. Here we report that one more protein fraction was found essential for the overall reaction and, more specifically, for formation of the methylated MT1 intermediate. The more of the protein that was present, the shorter the delay of the start of methyl group transfer. The maximum velocity of methyl transfer was not substantially affected by these varying amounts of protein. This demonstrated that the protein was involved in the activation of MT1. Therefore, it was called methyltransferase activation protein.
Daas
P J
PJ
Department of Microbiology, Faculty of Science, University of Nijmegen, The Netherlands.
Gerrits
K A
KA
Keltjens
J T
JT
van der Drift
C
C
Vogels
G D
GD
D000255:Adenosine Triphosphate / Q000378:metabolism; D001426:Bacterial Proteins / Q000378:metabolism*; D002852:Chromatography, Ion Exchange; D004789:Enzyme Activation; D007700:Kinetics; D015080:Mesna / Q000378:metabolism; D000432:Methanol / Q000378:metabolism; D017021:Methanosarcina barkeri / Q000201:enzymology*; D008745:Methylation; D008780:Methyltransferases / Q000378:metabolism*
D001426:Bacterial Proteins; D000255:Adenosine Triphosphate; D008780:Methyltransferases; C036890:methanol-2-mercaptoethanesulfonic acid methyltransferase; C040528:methanol-5-hydroxybenzimidazolylcobamide methyltransferase; D015080:Mesna; D000432:Methanol