title: U1 small nuclear ribonucleoprotein particle-protein interactions are revealed in Saccharomyces cerevisiae by in vivo competition assays.
abstract: Two highly conserved regions of the 586-nucleotide yeast (Saccharomyces cerevisiae) U1 small nuclear RNA (snRNA) can be mutated or deleted with little or no effect on growth rate: the universally conserved loop II (corresponding to the metazoan A loop) and the yeast core region (X. Liao, L. Kretzner, B. Séraphin, and M. Rosbash, Genes Dev. 4:1766-1774, 1990). To examine the contribution of these regions to U1 small nuclear ribonucleoprotein particle (snRNP) activity, a competitor U1 gene, encoding a nonfunctional U1 snRNA molecule, was introduced into a number of strains carrying a U1 snRNA gene with loop II or yeast core mutations. The presence of the nonfunctional U1 gene lowered the growth rate of these mutant strains but not wild-type strains, consistent with the notion that mutant U1 RNAs are less active than wild-type U1 snRNAs. A detailed analysis of the U1 snRNA levels and half-lives in a number of merodiploid strains suggests that these mutant U1 snRNAs interact with U1 snRNP proteins less well than do their wild-type counterparts. Competition for protein factors during snRNP assembly could account for a number of previous observations in both yeast and mammalian cells.
authors.affiliation: Howard Hughes Medical Institute, Department of Biology, Brandeis University, Waltham, Massachusetts 02254.
authors.forename: F
authors.initials: F
authors.lastname: Stutz
authors.forename: X C
authors.initials: XC
authors.lastname: Liao
authors.forename: M
authors.initials: M
authors.lastname: Rosbash
mesh_terms: D001483:Base Sequence; D006860:Hydrogen Bonding; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D012331:RNA, Fungal / Q000737:chemistry; D012342:RNA, Small Nuclear / Q000737:chemistry / Q000502:physiology*; D017412:Ribonucleoprotein, U1 Small Nuclear / Q000737:chemistry / Q000502:physiology*; D012441:Saccharomyces cerevisiae / Q000235:genetics*
chemical_list: D046911:Macromolecular Substances; D012331:RNA, Fungal; D012342:RNA, Small Nuclear; D017412:Ribonucleoprotein, U1 Small Nuclear