A novel Euglena gracilis chloroplast operon encoding four ATP synthase subunits and two ribosomal proteins contains 17 introns.
The structure of a Euglena gracilis chloroplast operon encoding four subunits of the chloroplast ATP synthase complex and two ribosomal proteins has been determined. These six genes contain 17 introns. This operon is transcribed as a hexacistronic primary transcript which is subsequently processed to monocistronic mRNAs. The linear order of these genes, 5'-rps2-atpI-atpH-atpF-atpA-rps18-3' , encoding ribosomal protein S2, chloroplast ATP synthase subunits CF0IV, CF0III, CF0I, CF1 alpha and ribosomal protein S18, respectively, is similar to the equivalent operons of prokaryotes, cyanelles and land-plant chloroplasts. This operon differs from those of these other organisms in the co-transcription of rps18 and in intron content.
Drager
R G
RG
Department of Molecular and Cellular Biology, University of Arizona, Tucson 85721.
Hallick
R B
RB
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002736:Chloroplasts; D003001:Cloning, Molecular; D005056:Euglena gracilis / Q000235:genetics*; D015870:Gene Expression; D017343:Genes, Plant; D006860:Hydrogen Bonding; D007438:Introns; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009876:Operon; D016133:Polymerase Chain Reaction; D006180:Proton-Translocating ATPases / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D015183:Restriction Mapping; D012269:Ribosomal Proteins / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic
D012333:RNA, Messenger; D012269:Ribosomal Proteins; D006180:Proton-Translocating ATPases