Dual Alu polymerase chain reaction primers and conditions for isolation of human chromosome painting probes from hybrid cells.
A method for rapid and efficient production of chromosome- and chromosome-region specific probes for fluorescent in situ hybridization (FISH) detectable by simple fluorescent microscopy is described. The procedure is based on simultaneous use of two inter-Alu-polymerase chain reaction (PCR) primers for extraction of highly heterogeneous human DNA from interspecific somatic cell hybrids containing the chromosome regions of interest. Probes so produced do not hybridize to centromeric sequences and simultaneously band the target chromosomes, making them useful for unambiguous identification of chromosomal elements and breakpoints associated with cancer.
Liu
P
P
Department of Molecular Genetics, University of Texas M. D. Anderson Cancer Center, Houston 77030.
Siciliano
J
J
Seong
D
D
Craig
J
J
Zhao
Y
Y
de Jong
P J
PJ
Siciliano
M J
MJ
D000818:Animals; D001483:Base Sequence; D016466:CHO Cells; D002871:Chromosome Banding; D017124:Conserved Sequence / Q000235:genetics; D006224:Cricetinae; D015342:DNA Probes / Q000737:chemistry / Q000302:isolation & purification*; D006801:Humans; D006822:Hybrid Cells; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008969:Molecular Sequence Data; D021141:Nucleic Acid Amplification Techniques; D016133:Polymerase Chain Reaction / Q000379:methods*; D012091:Repetitive Sequences, Nucleic Acid
D015342:DNA Probes