Chromosomal DNA probes for the identification of Bacteroides tectum and Bacteroides fragilis from the oral cavity of cats.
A dot-blot hybridisation assay using high molecular weight DNA as whole chromosomal probes was used to differentiate Bacteroides tectum from Bacteroides fragilis. 32P-labelled probes were compared with digoxigenin (DIG)-labelled probes. The whole chromosomal probes were specific--differentiating B. tectum from B. fragilis and both from a variety of other species (including other members of the genera Bacteroides, Fusobacterium, Eubacterium, and Prevotella) found in normal and abnormal mouths of cats and horses. However, even at very high stringencies, B. tectum homology groups I, II and III were not distinguishable from one another using either 32P-labelled or DIG-labelled probes. Thus, DIG-labelled whole chromosome probes directed against cellular DNA released directly onto nitrocellulose membranes is considered a useful method for diagnostic veterinary laboratories wishing to identify B. tectum and distinguish it from B. fragilis and other oral anaerobic flora of cats.
Love
D N
DN
Department of Veterinary Pathology, University of Sydney, NSW Australia.
Bailey
G D
GD
D000818:Animals; D001439:Bacteroides / Q000145:classification / Q000302:isolation & purification*; D001441:Bacteroides fragilis / Q000302:isolation & purification; D002371:Cat Diseases / Q000382:microbiology*; D002415:Cats; D015342:DNA Probes; D004269:DNA, Bacterial / Q000302:isolation & purification*; D005882:Gingival Diseases / Q000382:microbiology / Q000662:veterinary*; D013045:Species Specificity
D015342:DNA Probes; D004269:DNA, Bacterial