Smooth muscle alpha-actin promoter activity is induced by serum stimulation of fibroblast cells.
Transient transfection analysis of a plasmid containing human alpha-actin promoter linked to bacterial chloramphenicol acetyl transferase (CAT) reporter gene, in Rat-2 fibroblast cells, indicates that smooth muscle (sm) alpha-actin promoter activity is induced by serum. The CArG [CC (A/T) 6GG] box sequences within the alpha-actin promoter mediate the serum induction. This induction can be blocked by overexpression of Fos, suggesting that it follows the same regulatory pathway as c-fos.
Kim
J H
JH
Department of Tumor Biology, Schering-Plough Research Institute, Kenilworth, New Jersey 07033.
Bushel
P R
PR
Kumar
C C
CC
D000199:Actins / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D005347:Fibroblasts / Q000502:physiology; D005786:Gene Expression Regulation; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D009130:Muscle, Smooth / Q000502:physiology*; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D011401:Promoter Regions, Genetic; D016755:Proto-Oncogene Proteins c-jun / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats
D000199:Actins; D009838:Oligodeoxyribonucleotides; D016755:Proto-Oncogene Proteins c-jun; D012333:RNA, Messenger