Polymerase chain reaction-amplified nonradioactive probes for identification of Fusobacterium nucleatum.
A polymerase chain reaction probe with 100% sequence identity to 120 deoxyribonucleotides of Fusobacterium nucleatum Fev1, coding for a part of the 40-kDa major outer membrane protein, was labeled with the steroid hapten digoxigenin. The probe was compared with various degenerate oligonucleotide probes and found to tolerate much more stringent washing conditions. It was therefore superior in distinguishing, by means of Southern blots and slot blots, F. nucleatum from other oral gram-negative bacteria in the periodontal pocket and from other fusobacterial species and in distinguishing among different strains of F. nucleatum. F. periodonticum was found to be more similar to F. nucleatum than the other fusobacterial species tested.
Bolstad
A I
AI
Department of Biochemistry, School of Dentistry, University of Bergen, Norway.
Jensen
H B
HB
D015139:Blotting, Southern / Q000379:methods; D015342:DNA Probes; D004076:Digoxigenin; D005673:Fusobacterium / Q000145:classification / Q000302:isolation & purification; D016967:Fusobacterium nucleatum / Q000235:genetics / Q000302:isolation & purification*; D009693:Nucleic Acid Hybridization / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods*
D015342:DNA Probes; D004076:Digoxigenin