title: Mass spectrometric investigation of the presence of 7-methyl ring-opened guanine derivatives in urine.
abstract: The involvement of chemical alkylating agents in tumorigenesis and chemotherapy is well established and it was shown that one of the main sites of alkylation is the N-7 of guanine in DNA. Though excision of damaged bases is regarded as one of the repair mechanisms in damaged DNA there is a scarcity of information concerning the excised final metabolites in body fluids. This study attempts to demonstrate the usefulness of CAD MS/MS for the detection of the final metabolite-deformylated ring-opened 7 alkylguanine in urine. Such mass spectrometric methods can be used in biomedical studies.
authors.affiliation: Department of Analytical Chemistry, Israel Institute for Biological Research, Ness-Ziona.
authors.forename: R
authors.initials: R
authors.lastname: Barak
authors.forename: A
authors.initials: A
authors.lastname: Vincze
authors.forename: P
authors.initials: P
authors.lastname: Bel
authors.forename: S P
authors.initials: SP
authors.lastname: Dutta
authors.forename: G B
authors.initials: GB
authors.lastname: Chedda
mesh_terms: D000478:Alkylation; D004249:DNA Damage; D004260:DNA Repair; D008401:Gas Chromatography-Mass Spectrometry; D006147:Guanine / Q000031:analogs & derivatives* / Q000652:urine; D006801:Humans; D013058:Mass Spectrometry; D008745:Methylation
chemical_list: D006147:Guanine; C008450:7-methylguanine