Immunological Identification of the Shaker-Related Kv1.3 Potassium Channel Protein in T and B Lymphocytes, and Detection of Related Proteins in Flies and Yeast
Shaker-related potassium (K+) channel proteins contain sequences which exhibit remarkable conservation across species. We have generated polyclonal anti-peptide antibodies (Abs) which cross-react with peptide epitopes of several Shaker-related channels (Kv1.1, 1.2 and 1.3), in addition to a Kv1.3-specific Ab. The Kv1.3-specific Abs react with a protein expressed in human T-cells (Jurkat and PBLs), as well as in mouse T-cells (EL-4) and pre-B cells (230.37). The cross-reactive Abs detect the Shaker protein in Drosophila melanogaster, in addition to an immunologically related protein in the yeast Saccharomyces cerevisiae. Abs which recognize these shared epitopes could serve for the identification and biochemical characterization of Shaker-related proteins in diverse organisms.
Spencer
R H
RH
Department of Microbiology and Molecular Genetics, University of California, Irvine 92717.
Chandy
K G
KG
Gutman
G A
GA
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000302:isolation & purification; D001402:B-Lymphocytes / Q000502:physiology*; D015153:Blotting, Western; D002460:Cell Line; D003429:Cross Reactions; D004331:Drosophila melanogaster / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008565:Membrane Proteins / Q000032:analysis* / Q000235:genetics / Q000302:isolation & purification; D051379:Mice; D008818:Mice, Neurologic Mutants / Q000235:genetics; D008969:Molecular Sequence Data; D008970:Molecular Weight; D005810:Multigene Family; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology; D015221:Potassium Channels / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D013601:T-Lymphocytes / Q000502:physiology*
D000906:Antibodies; D008565:Membrane Proteins; D010455:Peptides; D015221:Potassium Channels; D012333:RNA, Messenger