title: Histone H1 kinase activity in bovine oocytes following calcium stimulation.
abstract: The influence of number of Ca2+ stimulations on the profile of histone H1 kinase activity in bovine oocytes was investigated. A CA2+ stimulation consisted of transferring oocytes directly from culture medium to mannitol containing 100 microM Ca2+ and pulsing oocytes with a 0.2 kVcm-1, 20 microseconds discharge. One, three, or six Ca2+ stimulations were given, each 22 min apart. Oocytes were frozen from 0 to 8 hr after the first stimulation at indicated time points and assayed for histone H1 kinase activity. H1 kinase activity was quantified using a densitometer and expressed as a percent of activity in nonpulsed metaphase II oocytes. Stimulating oocytes in the absence of Ca2+ in the pulsing medium did not inactivate H1 kinase. In the presence of Ca2+, however, H1 kinase was rapidly inactivated after stimulation. A single stimulation decreased H1 kinase activity to 44% +/- 11% of its initial level in 1 hr. However, H1 kinase was dramatically reactivated at 2 hr after the stimulation and reached 122% +/- 22% of the initial activity at 6 hr. With three stimulations, basal H1 kinase activity was 21% +/- 3% and was obtained in 30 min. H1 kinase reactivation started at 4 hr after the first stimulation and level of activity reached 38% +/- 15% at 8 hr. Six stimulations also led to rapid H1 kinase inactivation and to a basal activity of 14% +/- 0.4%. With six stimulations, however, basal H1 kinase activity was maintained over at least 8 hr, and no reactivation occurred during this period. Basal H1 kinase activity obtained after six stimulations was similar to that of fertilized oocytes.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: GenMark, Inc., Salt Lake City, Utah 84108.
authors.forename: P
authors.initials: P
authors.lastname: Collas
authors.forename: E J
authors.initials: EJ
authors.lastname: Sullivan
authors.forename: F L
authors.initials: FL
authors.lastname: Barnes
mesh_terms: D000818:Animals; D002118:Calcium / Q000494:pharmacology*; D002417:Cattle; D002478:Cells, Cultured; D004790:Enzyme Induction / Q000187:drug effects; D005307:Fertilization in Vitro; D016200:Maturation-Promoting Factor / Q000096:biosynthesis*; D009865:Oocytes / Q000187:drug effects / Q000201:enzymology*; D015053:Zygote / Q000187:drug effects / Q000201:enzymology*
chemical_list: D016200:Maturation-Promoting Factor; D002118:Calcium