title: Comparison of fiberglass-based histamine assay with a conventional automated fluorometric histamine assay, case history, skin prick test, and specific serum IgE in patients with milk and egg allergic reactions.
abstract: A microfiberglass-based histamine assay (HRM) was compared with an automated fluorometric histamine assay (HRA). Twenty-four subjects with and 24 without a case history (CH) of milk and/or egg allergy were tested by HRM and HRA, skin prick test (SPT), and specific serum IgE (RAST). Six different concentrations of milk, egg, and anti-IgE were used to stimulate washed leukocytes (250 microliters for HRA) and whole blood samples (25 microliters for HRM) in parallel. When we compared scores representing basophil sensitivity, correlation coefficients (rs) were positive (r(anti-IgE) = 0.88, r(egg) = 0.95, r(milk) = 0.88, P < 0.001), but no significant correlations were found after exclusion of the negatives in both tests. In some individual dose-response curves, the scores obtained by HRM were shifted to higher allergen and anti-IgE concentrations. A high degree of concordance was found in positive and negative responses between the two tests: anti-IgE 91%, egg 92%, milk 86%. Finally, we found a good concordance between, on one side, HRM and, on the other, CH, SPT, and RAST (HRM vs. CH/SPT/RAST): egg 92/82/82%; milk 89/74/67%. We conclude that HRM is in good qualitative, but poor quantitative, agreement with the autoanalyzer-based fluorometric histamine assay.
authors.affiliation: Department of Clinical Immunology and Asthma OPD, University Clinic Rudolf Virchow, Freie Universität Berlin, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Kleine-Tebbe
authors.forename: S
authors.initials: S
authors.lastname: Werfel
authors.forename: D
authors.initials: D
authors.lastname: Roedsgaard
authors.forename: H
authors.initials: H
authors.lastname: Nolte
authors.forename: P S
authors.initials: PS
authors.lastname: Skov
authors.forename: U
authors.initials: U
authors.lastname: Wahn
authors.forename: G
authors.initials: G
authors.lastname: Kunkel
mesh_terms: D000293:Adolescent; D000328:Adult; D001491:Basophils / Q000276:immunology / Q000378:metabolism; D002648:Child; D002675:Child, Preschool; D004306:Dose-Response Relationship, Immunologic; D004531:Eggs / Q000009:adverse effects; D005470:Fluorometry / Q000379:methods*; D005512:Food Hypersensitivity / Q000175:diagnosis* / Q000276:immunology; D005898:Glass; D006632:Histamine / Q000032:analysis*; D006801:Humans; D007073:Immunoglobulin E / Q000032:analysis*; D008487:Medical History Taking; D008875:Middle Aged; D016269:Milk Hypersensitivity / Q000175:diagnosis* / Q000276:immunology; D011852:Radioallergosorbent Test; D012882:Skin Tests
chemical_list: C009753:fiberglass; D007073:Immunoglobulin E; D006632:Histamine