title: Molecular cloning of a mouse myosin I expressed in brain.
abstract: We have isolated two cDNAs that encode putative myosin I heavy chains by polymerase chain reaction amplification of brain cDNA with degenerate oligodeoxynucleotide primers representing myosin I-specific conserved amino acid sequences. We report the complete deduced amino acid sequence of one of these cDNAs. The sequences is most similar to those of the avian and bovine brush border myosin Is, with five putative calmodulin-binding repeats at the head-tail junction. Northern analysis demonstrates that this myosin heavy chain, unlike the brush border myosins, is expressed in many tissues.
authors.affiliation: Department of Physiology, University of Texas Southwestern Medical Center, Dallas 75235-9040.
authors.forename: J S
authors.initials: JS
authors.lastname: Koslovsky
authors.forename: C
authors.initials: C
authors.lastname: Qian
authors.forename: X
authors.initials: X
authors.lastname: Jiang
authors.forename: J A
authors.initials: JA
authors.lastname: Mercer
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D001921:Brain / Q000378:metabolism*; D003001:Cloning, Molecular; D017124:Conserved Sequence; D004247:DNA; D015870:Gene Expression; D051379:Mice; D008969:Molecular Sequence Data; D009218:Myosins / Q000737:chemistry / Q000235:genetics*; D009838:Oligodeoxyribonucleotides; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment
chemical_list: D009838:Oligodeoxyribonucleotides; D004247:DNA; D009218:Myosins