Interleukin-1 binding sites on astrocytes.
Interleukin-1 has been shown to have regulatory effects on glial cell functions. In this study, we examined the capacity of astroglial cells to specifically bind recombinant iodinated human interleukin-1 alpha. This was performed in mouse brain by both in situ and in vitro autoradiography, on areas of gliosis and on astrocytes and microglia primary and secondary cultures respectively. Specific binding was shown in the brain sections over areas of glial proliferation, and in addition, quantitative autoradiography was performed. Analysis of competition experiments by autoradiography led to EC50 values of 5 x 10(-11) M for human interleukin-1 alpha and approximately 10(-9) M for the interleukin-1 receptor antagonist. In cultures, iodinated human interleukin-1 alpha bound specifically to astrocytes but was unable to bind to microglial cells. Competition binding experiments in astrocyte cultures led to EC50 values of 8 x 10(-11) M and 1 x 10(-10) M for human interleukin-1 alpha and mouse interleukin-1 beta respectively, and an EC50 higher than 10(-9) M for the antagonist. The presence of interleukin-1 receptors on astroglial cells provides biochemical support for the various effects of interleukin-1 in the central nervous system, particularly those concerning the formation of scar tissue, possibly by astroglia proliferation after brain injury.
Ban
E M
EM
Unité de Pharmacologie Neuro-Immuno-Endocrinienne, Institut Pasteur, Paris, France.
Sarliève
L L
LL
Haour
F G
FG
D000818:Animals; D001253:Astrocytes / Q000276:immunology*; D001345:Autoradiography; D001667:Binding, Competitive; D001921:Brain / Q000276:immunology*; D002478:Cells, Cultured; D005260:Female; D007375:Interleukin-1 / Q000378:metabolism*; D007457:Iodine Radioisotopes; D007700:Kinetics; D051379:Mice; D008809:Mice, Inbred C3H; D017472:Receptors, Interleukin-1 / Q000378:metabolism*
D007375:Interleukin-1; D007457:Iodine Radioisotopes; D017472:Receptors, Interleukin-1