Human T cell lines and clones respond to IL-9.
The activity of IL-9 on human T cells was investigated. Human T cell lines and clones, derived from PBMC by stimulation with PHA, IL-2, and irradiated allogeneic PBMC, were found to express a strong IL-9R message at the RNA level and to proliferate in the presence of IL-9, irrespectively of their CD4+ or CD8+ phenotype. Moreover, tumor-specific CTL clones also responded to IL-9. Contrasting with other T cell growth factors, such as IL-2, IL-4, or IL-7, IL-9 did not induce the proliferation of freshly isolated T cells. However, a significant proliferative response to IL-9 could be observed after a 10-day activation of PBMC with PHA, IL-2, and feeders. Taken together, our results indicate 1) that the activity of IL-9 on human T cells is wider than initially anticipated on the basis of the data obtained so far with murine cells; and 2) that proliferative responses to IL-9 require previous activation.
Houssiau
F A
FA
Experimental Medicine Unit, University of Louvain, Belgium.
Renauld
J C
JC
Stevens
M
M
Lehmann
F
F
Lethe
B
B
Coulie
P G
PG
Van Snick
J
J
D002460:Cell Line; D002999:Clone Cells; D006801:Humans; D016906:Interleukin-9 / Q000494:pharmacology*; D008213:Lymphocyte Activation / Q000187:drug effects*; D010641:Phenotype; D011971:Receptors, Immunologic / Q000378:metabolism; D018123:Receptors, Interleukin; D053651:Receptors, Interleukin-9; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000187:drug effects / Q000276:immunology
C508045:IL9R protein, human; D016906:Interleukin-9; D011971:Receptors, Immunologic; D018123:Receptors, Interleukin; D053651:Receptors, Interleukin-9