title: Crystallization and preliminary X-ray analysis of a truncated tissue metalloproteinase inhibitor delta 128-194 TIMP-2.
abstract: Crystals of an active truncated form of a human tissue metallproteinase inhibitor (delta 128-194 TIMP-2), a protein implicated in a number of diseases of the connective tissues, have been grown in a form suitable for study by X-ray diffraction analysis. Crystals grow using polyethylene glycol 600 as a precipitant and are in space group P2(1)2(1)2, with cell dimensions a = 75.28 A, b = 100.77 A, c = 31.45 A. The asymmetric unit appears to contain two molecules of delta TIMP-2 with a Vm value of 2.3 A3/dalton. The crystals diffract to Bragg-spacings beyond 2.5 A. Native data to 3.2 A have been collected and a search for heavy-metal derivatives is in progress.
authors.affiliation: Department of Chemistry, University of York, Heslington, U.K.
authors.forename: S
authors.initials: S
authors.lastname: Tolley
authors.forename: G
authors.initials: G
authors.lastname: Murphy
authors.forename: M
authors.initials: M
authors.lastname: O'Shea
authors.forename: R
authors.initials: R
authors.lastname: Ward
authors.forename: A
authors.initials: A
authors.lastname: Docherty
authors.forename: M
authors.initials: M
authors.lastname: Cockett
authors.forename: A
authors.initials: A
authors.lastname: Rawas
authors.forename: G
authors.initials: G
authors.lastname: Davies
mesh_terms: D003460:Crystallization; D006801:Humans; D008666:Metalloendopeptidases / Q000737:chemistry*; D009363:Neoplasm Proteins / Q000737:chemistry*; D010446:Peptide Fragments / Q000737:chemistry; D019716:Tissue Inhibitor of Metalloproteinase-2; D014961:X-Ray Diffraction
chemical_list: D009363:Neoplasm Proteins; D010446:Peptide Fragments; D019716:Tissue Inhibitor of Metalloproteinase-2; D008666:Metalloendopeptidases