Direct demonstration of membrane IL-1 alpha on the surface of circulating B lymphocytes and monocytes.
A mAb against IL-1 alpha has been used in a high sensitivity immunofluorescence procedure to demonstrate directly the presence of surface IL-1 alpha on human monocytes and B cells from blood and tonsil. IL-1 alpha could be detected on monocytes and a subset (around 30%) of B cells without any in vitro activation. Although the subset of B cells expressing IL-1 alpha was distinct (i.e., the distribution of fluorescence intensity was bimodal), no correlation was seen with any other B cell subset marker in blood. In tonsil, IL-1 alpha was expressed by cells with a phenotype that suggested that they represented a subset of mantle zone B cells. Activation of tonsil B cells with anti-IgM, IL-4, IL-2, and mixtures of these reagents led to small increases in IL-1 alpha expression, as did activation of unfractionated tonsil cells with PHA.
Zola
H
H
Department of Clinical Immunology, Flinders Medical Centre, Adelaide, South Australia.
Flego
L
L
Wong
Y T
YT
Macardle
P J
PJ
Kenney
J S
JS
D000818:Animals; D015703:Antigens, CD / Q000032:analysis; D016175:B-Lymphocyte Subsets / Q000737:chemistry / Q000276:immunology; D001402:B-Lymphocytes / Q000737:chemistry*; D002462:Cell Membrane / Q000737:chemistry; D006801:Humans; D007375:Interleukin-1 / Q000032:analysis* / Q000097:blood; D007938:Leukemia / Q000378:metabolism; D008213:Lymphocyte Activation; D051379:Mice; D009000:Monocytes / Q000737:chemistry*; D014066:Palatine Tonsil / Q000737:chemistry / Q000166:cytology
D015703:Antigens, CD; D007375:Interleukin-1