Regulatory relevance of lipoproteins in interleukin-2 stimulated lymphocytes in vitro.
The regulatory role of very low density lipoproteins (VLDL) in de novo protein synthesis and in natural killer (NK) mediated cytotoxicity against cancer cells was evaluated in IL-2 activated peripheral blood mononuclear cells (PBL). Supplementing of VLDL to the incubation medium induced a potent dose-related inhibitory effect on the synthesis and secretion of (35S) labelled proteins. The maximal inhibitory response was observed at 50 micrograms/ml cholesterol VLDL. The effect of VLDL on the IL-2 induced ability of lymphocytes to kill cancer cells and to interfere with target cell proliferation was then evaluated. Maximal cytotoxicity was observed at 50 micrograms VLDL/ml as cholesterol. This VLDL-dependent stimulatory effect was associated with a significant decrease in the proliferative effect of the medium conditioned by PBL. The possibility that VLDL mediate the regulation of immune functions by interacting with metabolic patterns expressed by IL-2 stimulated lymphocytes is discussed in light of these results.
Brevini
T
T
Institute of Endocrinology, University of Milan, Italy.
Mantovani
M
M
Corsini
A
A
Gambetta
A
A
Gandolfi
F
F
Bozzetti
F
F
Agradi
E
E
D000328:Adult; D002478:Cells, Cultured; D003602:Cytotoxicity, Immunologic / Q000187:drug effects / Q000276:immunology; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology*; D007694:Killer Cells, Natural / Q000187:drug effects / Q000276:immunology; D008079:Lipoproteins, VLDL / Q000502:physiology*; D008214:Lymphocytes / Q000187:drug effects* / Q000276:immunology; D014176:Protein Biosynthesis; D011506:Proteins / Q000378:metabolism; D014407:Tumor Cells, Cultured
D007376:Interleukin-2; D008079:Lipoproteins, VLDL; D011506:Proteins