Evidence for a membrane skeleton in higher plants. A spectrin-like polypeptide co-isolates with rice root plasma membranes.
A fraction enriched in plasma membranes was isolated from rice roots by differential centrifugation and aqueous polymer two-phase partitioning. Analysis of the fraction by SDS-PAGE showed the presence of several low mobility polypeptides (M(r) > 100 kDa). One of these polypeptides (M(r) approximately 230 kDa) was specifically recognized by polyclonal antibodies to human erythrocyte spectrin. This finding suggests that a higher plant spectrin-based membrane skeleton may be preserved and studied using high-purity plasma membrane fractions obtained by aqueous polymer two-phase partitioning.
Faraday
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CD
Section of Plant Biology, Cornell University, Ithaca, NY 14853.
Spanswick
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RM
D000251:Adenosine Triphosphatases / Q000037:antagonists & inhibitors / Q000378:metabolism; D001386:Azides / Q000494:pharmacology; D002458:Cell Fractionation; D002462:Cell Membrane / Q000737:chemistry / Q000648:ultrastructure*; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D015151:Immunoblotting; D008861:Microsomes / Q000201:enzymology; D008928:Mitochondria / Q000201:enzymology; D008982:Molybdenum / Q000494:pharmacology; D009566:Nitrates / Q000494:pharmacology; D012275:Oryza / Q000648:ultrastructure; D010944:Plants / Q000648:ultrastructure*; D013049:Spectrin / Q000276:immunology / Q000302:isolation & purification*; D014638:Vanadates / Q000494:pharmacology
D001386:Azides; D009566:Nitrates; D013049:Spectrin; C044659:molybdate; D014638:Vanadates; D008982:Molybdenum; D000251:Adenosine Triphosphatases