Observation of living cells using the atomic force microscope.
We used an atomic force microscope (AFM) to image samples immersed in a fluid in order to study the dynamic behavior of the membranes of living cells. AFM images of cultured cells immersed in a buffer were obtained without any preliminary preparation. We observed surface changes and displacements which suggest that the cells were still alive during the measurements. Some membrane details imaged with the AFM have also been observed using a scanning electron microscope and their dynamic behavior has been confirmed by microcinematography. We believe that the AFM will offer new insights into the exploration of dynamic changes affecting cell membranes.
Kasas
S
S
Institute of Histology, University of Fribourg, Switzerland.
Gotzos
V
V
Celio
M R
MR
D055592:Biophysical Phenomena; D001703:Biophysics; D002460:Cell Line; D002477:Cells / Q000648:ultrastructure*; D003584:Cytological Techniques; D005069:Evaluation Studies as Topic; D006801:Humans; D008853:Microscopy / Q000295:instrumentation / Q000379:methods*; D008855:Microscopy, Electron, Scanning