title: Caprine plasma proteinase inhibitors--I. Partial characterization.
abstract: 1. Using two-dimensional electrophoresis (IEF, pH 3.5-6.0 and PAGE, 11.5% T, pH 7.9) the caprine plasma proteinase inhibitors were classified into six distinct classes, designated PIA, PIB, PIC, PID, PIE and PIF. Differentiation of the six inhibitors was based on electrophoretic criteria, their abilities to inhibit bovine trypsin and chymotrypsin and their crossreactions with antisera to human alpha 1-antitrypsin and alpha 1-antichymotrypsin. 2. Polymorphic variants were identified for five of the protein systems (PIA, PIB, PIC, PID and PIE) and the electrophoretic data indicated that the variants were controlled by allelic genes. PIF proteins were poorly resolved and invariant. 3. Treatment of selected plasmas with neuraminidase demonstrated that the microheterogeneity observed in the PIA, PIB, PIC and PID proteins was attributable to sialic acid additions. 4. The inhibitory activities of all six caprine proteinase inhibitors were unaffected by chemical oxidation with chloramine-T.
authors.affiliation: Department of Physiology and Pharmacology, University of Queensland, St Lucia, Brisbane, Australia.
authors.forename: D M
authors.initials: DM
authors.lastname: Vankan
authors.forename: K
authors.initials: K
authors.lastname: Bell
mesh_terms: D000818:Animals; D002700:Chloramines; D002918:Chymotrypsin / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D014644:Genetic Variation; D006041:Goats / Q000097:blood*; D007525:Isoelectric Focusing; D009439:Neuraminidase / Q000494:pharmacology; D010084:Oxidation-Reduction; D011480:Protease Inhibitors / Q000097:blood*; D014105:Tosyl Compounds; D014357:Trypsin / Q000378:metabolism
chemical_list: D002700:Chloramines; D011480:Protease Inhibitors; D014105:Tosyl Compounds; C016300:chloramine-T; D009439:Neuraminidase; D002918:Chymotrypsin; D014357:Trypsin