Sensitive high-performance liquid chromatographic assay for praziquantel in plasma, urine and liver homogenates.
A high-performance liquid chromatographic method for the determination of praziquantel in plasma, urine and rat liver homogenates has been developed. It requires 2 ml of biological fluid, an extraction using Sep-Pak cartridges, a 0.05 M phosphate buffer solution (pH 5.0) for equilibrating and washing and ethyl acetate-diisopropyl ether for drug elution. The analysis was performed on an Ultrasphere ODS C18 column with a mobile phase of acetonitrile-water with ultraviolet detection at 217 nm. The results showed that the assay is sensitive (31.2 ng/ml), linear between 0.125 and 4.0 micrograms/ml, precise (coefficient of variation = 10%) and selective with other drugs currently administered with praziquantel.
González-Esquivel
D F
DF
Laboratory of Neuropharmacology, National Institute of Neurology and Neurosurgery of Mexico, Mexico City.
Okuno
C M
CM
Sánchez Rodríguez
M
M
Solelo Morales
J
J
Cook
H J
HJ
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D008099:Liver / Q000737:chemistry*; D011223:Praziquantel / Q000032:analysis* / Q000097:blood / Q000652:urine; D051381:Rats; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet
D011223:Praziquantel