Cell-dependent requirement of human immunodeficiency virus type 1 Vif protein for maturation of virus particles.
A highly sensitive single-round infection assay using a bacterial chloramphenicol acetyltransferase was developed to analyze an early stage of human immunodeficiency virus type 1 replication. By a combination of transfection and single-round infection assay, a virus with a vif mutation, depending on host cells from which the virus was derived, was demonstrated to be defective at the early phase of infection cycle. Analysis of viral proteins synthesized in cells indicated that incorporation of the Env surface protein into virions of the vif mutant, again in a cell-dependent way, was greatly restricted. Taken together, it is concluded that the Vif protein acts through modulation of the Env protein in the virions, directly or indirectly, to enhance viral infectivity in a certain cell type.
Sakai
H
H
Institute for Virus Research, Kyoto University, Japan.
Shibata
R
R
Sakuragi
J
J
Sakuragi
S
S
Kawamura
M
M
Adachi
A
A
D000163:Acquired Immunodeficiency Syndrome / Q000378:metabolism*; D000818:Animals; D002415:Cats; D002459:Cell Fusion; D002478:Cells, Cultured; D003673:Defective Viruses / Q000254:growth & development; D015686:Gene Products, env / Q000096:biosynthesis; D015683:Gene Products, gag / Q000096:biosynthesis; D016346:Gene Products, vif / Q000235:genetics*; D005819:Genetic Markers; D015497:HIV-1 / Q000254:growth & development*; D014162:Transfection; D014771:Virion / Q000254:growth & development; D014779:Virus Replication; D054320:vif Gene Products, Human Immunodeficiency Virus
D015686:Gene Products, env; D015683:Gene Products, gag; D016346:Gene Products, vif; D005819:Genetic Markers; D054320:vif Gene Products, Human Immunodeficiency Virus