Tyrosine kinase activity is involved in the protein kinase C induced expression of interleukin-1 beta gene in monocytic cells.
The role of protein tyrosine kinases in the expression of interleukin-1 beta (IL-1 beta) gene in response to phorbol esters (PMA) in THP-1 cell line was investigated. Genistein, a specific tyrosine kinase inhibitor, inhibited PMA induced IL-1 beta protein and mRNA levels in THP-1 cells. Genistein did not have a significant effect on PMA induced activity in transient transfection assays using reporter gene constructs containing the PMA responsive sequence of the IL-1 beta gene linked to IL-1 beta promoter, or five repeats of PMA responsive sites (AP-1 sites) in front of a thymidine kinase promoter. This indicates that the tyrosine kinase activity required for the PMA induced IL-1 beta expression is coupled downstream from or functions independent of the PMA induced AP-1 activity.
Palkama
T
T
Department of Bacteriology and Immunology, University of Helsinki, Finland.
Matikainen
S
S
Hurme
M
M
D002460:Cell Line; D004742:Enhancer Elements, Genetic; D015870:Gene Expression / Q000187:drug effects; D019833:Genistein; D007375:Interleukin-1 / Q000235:genetics*; D007529:Isoflavones / Q000494:pharmacology; D009000:Monocytes / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D011493:Protein Kinase C / Q000378:metabolism*; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014162:Transfection
D007375:Interleukin-1; D007529:Isoflavones; D012333:RNA, Messenger; D019833:Genistein; D011505:Protein-Tyrosine Kinases; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate