title: Crystallization and preliminary X-ray analysis of an NAD(+)-dependent alcohol dehydrogenase from the extreme thermophilic archaebacterium Sulfolobus solfataricus.
abstract: An NAD(+)-dependent alcohol dehydrogenase from the extreme thermophilic archaebacterium Sulfolobus solfataricus has been crystallized in the holo-enzyme and apo-enzyme forms. Crystals of the holo-enzyme grow from 2-methyl-2,4-pentanediol at pH 8.4 with the addition of NADH and at pH 7.0 with the addition of NADH and dimethyl sulphoxide. Crystals of the apo-enzyme grow at pH 6.3 from a mixture of polyethylene glycol 4000 and propan-2-ol. The holo-enzyme crystallizes in C2 with a dimer in the asymmetric unit, however the crystals are twinned and unsuitable for data collection. The apo-enzyme crystallizes in I4(1)22 (a = 126.82 A, b = 118.95 A) with a monomer in the asymmetric unit, and the single crystals diffract to 2.8 A.
authors.affiliation: Department of Biochemistry and Molecular Biology, University College London, U.K.
authors.forename: L H
authors.initials: LH
authors.lastname: Pearl
authors.forename: D
authors.initials: D
authors.lastname: Demasi
authors.forename: A M
authors.initials: AM
authors.lastname: Hemmings
authors.forename: F
authors.initials: F
authors.lastname: Sica
authors.forename: L
authors.initials: L
authors.lastname: Mazzarella
authors.forename: C A
authors.initials: CA
authors.lastname: Raia
authors.forename: S
authors.initials: S
authors.lastname: D'Auria
authors.forename: M
authors.initials: M
authors.lastname: Rossi
mesh_terms: D000426:Alcohol Dehydrogenase / Q000737:chemistry* / Q000378:metabolism; D009243:NAD / Q000378:metabolism*; D016944:Sulfolobus / Q000201:enzymology*; D014961:X-Ray Diffraction
chemical_list: D009243:NAD; D000426:Alcohol Dehydrogenase