Development of immortalized human articular cartilage cell lines.
Human articular cartilage cells were transfected with the t.-sensitive polyomavirus large T antigen of SV40. Several immortalized chondrocyte cell lines were obtained. The types of acidic polysaccharides and of collagen synthesized suggest dedifferentiation in the in vitro culture system used afterwards to obtain large numbers of cells.
Verbruggen
G
G
Dept. of Rheumatology, Ghent University Hospital, Belgium.
Malfait
A M
AM
Almqvist
K F
KF
Veys
E M
EM
Thenet
S
S
Benoit
B
B
Demignot
S
S
Tachet des Combes
A
A
Adolphe
M
M
D000952:Antigens, Polyomavirus Transforming / Q000276:immunology; D002358:Cartilage, Articular / Q000166:cytology* / Q000276:immunology; D002454:Cell Differentiation; D002460:Cell Line; D002852:Chromatography, Ion Exchange; D003094:Collagen / Q000378:metabolism; D005270:Femur Head / Q000166:cytology; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry / Q000378:metabolism; D010641:Phenotype; D011509:Proteoglycans / Q000737:chemistry / Q000378:metabolism; D014162:Transfection
D000952:Antigens, Polyomavirus Transforming; D011509:Proteoglycans; D006820:Hyaluronic Acid; D003094:Collagen