Chromosomal reassignment: YACs containing both YES1 and thymidylate synthase map to the short arm of chromosome 18.
The YES1 proto-oncogene was mapped previously to human chromosome band 18q21.3 by using isotopic in situ hybridization. Using yeast artificial chromosomes (YACs) as probes and fluorescence in situ hybridization, a strong signal was detected in the region corresponding to 18p11.3. Restriction digests confirmed that the YACs contained the YES1 gene and not other cross-hybridizing, protein-tyrosine kinases. In addition, these YACs were found to contain another 18p11.32 gene, thymidylate synthase. These genes were less than 50 kb apart. Collectively, these data suggest that YES1 maps to 18p11.32 rather than to 18q21.3.
Silverman
G A
GA
Department of Pediatrics, Joint Program in Neonatology, Harvard Medical School, Boston, Massachusetts 02115.
Kuo
W L
WL
Taillon-Miller
P
P
Gray
J W
JW
D001483:Base Sequence; D002874:Chromosome Mapping; D015825:Chromosomes, Fungal; D002887:Chromosomes, Human, Pair 18; D004247:DNA; D015894:Genome, Human; D015698:Genomic Library; D006801:Humans; D008969:Molecular Sequence Data; D011505:Protein-Tyrosine Kinases / Q000235:genetics*; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D051575:Proto-Oncogene Proteins c-yes; D015183:Restriction Mapping; D013940:Thymidylate Synthase / Q000235:genetics*; D019061:src-Family Kinases
C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins; D004247:DNA; D013940:Thymidylate Synthase; D011505:Protein-Tyrosine Kinases; D051575:Proto-Oncogene Proteins c-yes; D019061:src-Family Kinases