title: Determination of patulin by reversed-phase high-performance liquid chromatography with extraction by diphasic dialysis.
abstract: A simple and economical method has been developed for the determination of patulin in apple juice. The sample is extracted with ethyl acetate in a diphasic dialysis system, and the extract is cleaned up by elution from a Sep-Pak cartridge. Patulin is detected and determined by reversed-phase high-performance liquid chromatography using a Novapak C18 column and an ultraviolet detector. The lower detection limit is 1 microgram l-1 and the recovery is 85% at the 20 micrograms l-1 level.
authors.affiliation: Departamento Patología Animal I, Facultad de Veterinaria, Universidad Complutense, Madrid, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Prieta
authors.forename: M A
authors.initials: MA
authors.lastname: Moreno
authors.forename: J
authors.initials: J
authors.lastname: Bayo
authors.forename: S
authors.initials: S
authors.lastname: Díaz
authors.forename: G
authors.initials: G
authors.lastname: Suárez
authors.forename: L
authors.initials: L
authors.lastname: Domínguez
authors.forename: R
authors.initials: R
authors.lastname: Canela
authors.forename: V
authors.initials: V
authors.lastname: Sanchis
mesh_terms: D000085:Acetates; D001628:Beverages / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003956:Dialysis; D005638:Fruit / Q000737:chemistry*; D008832:Microchemistry; D010365:Patulin / Q000032:analysis*
chemical_list: D000085:Acetates; C007650:ethyl acetate; D010365:Patulin