title: Formation in vivo, purification and crystallization of a complex of the gamma and epsilon subunits of the F0F1-ATPase of Escherichia coli.
abstract: A complex comprising the epsilon subunit of Escherichia coli F1-ATPase (ECF1-ATPase) and a glutathione-S-transferase gamma subunit (of ECF1-ATPase) fusion protein was formed in vivo and purified from cell extracts by binding to glutathione-agarose beads. The glutathione-S-transferase was released from the complex by digestion with thrombin and the gamma/epsilon complex purified by cation-exchange chromatography. Crystals of the complex were grown by vapour diffusion using PEG8000 as precipitant. The crystals are orthorhombic, space-group P2(1)2(1)2 with a = 161.9 A, b = 44.1 A and c = 63.4 A. The volume of the asymmetric unit is consistent with the presence of a complex of one gamma subunit and one epsilon subunit.
authors.affiliation: Department of Biochemistry and Molecular Biology, John Curtin School of Medical Research, Australian National University, Canberra City, A.C.T.
authors.forename: G B
authors.initials: GB
authors.lastname: Cox
authors.forename: B A
authors.initials: BA
authors.lastname: Cromer
authors.forename: J M
authors.initials: JM
authors.lastname: Guss
authors.forename: I
authors.initials: I
authors.lastname: Harvey
authors.forename: P D
authors.initials: PD
authors.lastname: Jeffrey
authors.forename: R G
authors.initials: RG
authors.lastname: Solomon
authors.forename: D C
authors.initials: DC
authors.lastname: Webb
mesh_terms: D002852:Chromatography, Ion Exchange; D003460:Crystallization; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D005982:Glutathione Transferase / Q000235:genetics; D010957:Plasmids; D006180:Proton-Translocating ATPases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics
chemical_list: D011993:Recombinant Fusion Proteins; D005982:Glutathione Transferase; D006180:Proton-Translocating ATPases