Thyrotropin receptor in non-thyroid tissues.
Thyrotropin receptor (TSH-R), the main target for the autoantibody in Graves' disease, has been thought to be a thyroid-specific protein. However, we successfully obtained the cDNA fragments of TSH-R from rat retro-orbital tissues and adipose tissues by using polymerase chain reaction methods. Sequencing has revealed that the nucleotide sequence of the cDNAs from these non-thyroid tissues was identical to that from the thyroid. TSH-R peptide antibody, which recognizes rat TSH-R, stained a 104 kDa protein from the retro-orbital tissues and the adipose tissues. The band was not detected with the antibody preabsorbed with the peptide. These results suggest that the message is translated to make a TSH-R protein even in these non-thyroid tissues.
Endo
T
T
Third Department of Internal Medicine, University of Yamanashi Medical School, Japan.
Ohno
M
M
Kotani
S
S
Gunji
K
K
Onaya
T
T
D000818:Animals; D001483:Base Sequence; D015870:Gene Expression; D008297:Male; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D011989:Receptors, Thyrotropin / Q000235:genetics / Q000378:metabolism*; D014018:Tissue Distribution
D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger; D011989:Receptors, Thyrotropin