Overexpression of wild-type p53 and c-Myc in human fetal cells transformed with adenovirus early region 1.
The expression of p53 in a large panel of adenovirus (Ad) 2/5- and 12-transformed human, rat, and mouse cells has been examined. In all cases, in the absence of the larger Ad E1B protein, the level of p53 is very low. In human and rat cells when the Ad 12 E1B 54K polypeptide is expressed, p53 is much more abundant, although this is not the case in Ad 12 E1-transformed mouse cells. We conclude that expression of p53 is determined by virus serotype, host cell type, and viral proteins expressed. p53 in Ad 12 E1-transformed human cells is wild type but has an extended half-life. Stabilization is not through protein-protein interaction with the Ad E1B protein. The level of expression of c-Myc is also elevated in Ad-transformed human cells but this does not correlate with the presence of the E1B protein or with p53. However, Northern blot analysis indicates a direct correlation between mRNA and protein levels. We conclude that c-Myc is regulated at the transcriptional level, whereas p53 is regulated at the post-translational level in adenovirus transformants.
Grand
R J
RJ
Department of Cancer Studies, Medical School, University of Birmingham, United Kingdom.
Lecane
P S
PS
Roberts
S
S
Grant
M L
ML
Lane
D P
DP
Young
L S
LS
Dawson
C W
CW
Gallimore
P H
PH
D017501:Adenovirus E1B Proteins / Q000235:genetics / Q000378:metabolism*; D000260:Adenoviruses, Human / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral; D004279:DNA, Viral / Q000235:genetics; D005333:Fetus; D015870:Gene Expression; D016259:Genes, myc; D016158:Genes, p53; D006801:Humans; D007700:Kinetics; D051379:Mice; D008969:Molecular Sequence Data; D016271:Proto-Oncogene Proteins c-myc / Q000096:biosynthesis / Q000235:genetics*; D051381:Rats; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis / Q000235:genetics*
D017501:Adenovirus E1B Proteins; D004279:DNA, Viral; D016271:Proto-Oncogene Proteins c-myc; D016159:Tumor Suppressor Protein p53