Interleukin-1 and naproxen down-regulate the expression of IL-1 receptors in cultured human rheumatoid synovial cells (HRSC).
Using affinity binding of [125I]-IL-1 alpha and Scatchard analysis, we demonstrate here that exposure of cultured synovial cells (HRSC) to NPX (10(-4) M) for 96 h decreased the binding of IL-1 by 20 to 35%. This effect results from a down-regulation of the IL-1 receptors without change in the apparent binding affinity (kD: 770 pM). Pretreatment of cultures with IL-1 alpha (500 pg/ml) reduced the total binding of [125I]-IL-1 alpha on HRSC by 65%, indicating that IL-1 decreases the expression of its own receptors.
Pronost
S
S
Laboratoire de Biochimie du Tissu Conjonctif, C.H.U. Niveau 3, Caen, France.
Delecoeuillerie
G
G
Rédini
F
F
Paolozzi
L
L
Vivien
D
D
Galéra
P
P
Loyau
G
G
Pujol
J P
JP
D001172:Arthritis, Rheumatoid / Q000473:pathology*; D015536:Down-Regulation / Q000187:drug effects*; D006801:Humans; D007375:Interleukin-1 / Q000378:metabolism / Q000494:pharmacology*; D007457:Iodine Radioisotopes; D007700:Kinetics; D009288:Naproxen / Q000494:pharmacology*; D017472:Receptors, Interleukin-1 / Q000187:drug effects* / Q000378:metabolism; D011994:Recombinant Proteins / Q000494:pharmacology; D013583:Synovial Membrane / Q000473:pathology*
D007375:Interleukin-1; D007457:Iodine Radioisotopes; D017472:Receptors, Interleukin-1; D011994:Recombinant Proteins; D009288:Naproxen