Detection of Aeromonas hydrophila in food with an enzyme-linked immunosorbent assay.
A microtitration plate, antibody capture, enzyme-linked immunosorbent assay was developed for the detection of Aeromonas hydrophila serotype O: 11 (highly virulent strains). The assay utilizes a detector antibody which shows no cross-reactions with Aeromonas strains other than serotype O: 11 or non-Aeromonas competing organisms. The detector antibody is mixed with the sample and incubated for 1 h, microcentrifuged and the supernatant fluid (unadsorbed antibody) titred in a microtitre plate coated with A. hydrophila cells from serotype O: 11. All the A. hydrophila strains from serotype O: 11 tested reacted strongly with the detector antibody. Also by culturing and performing the immunoassay with the detector antibody we established and quantified the presence of A. hydrophila O: 11 in different foods.
Merino
S
S
Departamento de Microbiologia, Universidad de Barcelona, Spain.
Camprubí
S
S
Tomás
J M
JM
D016980:Aeromonas hydrophila / Q000276:immunology / Q000302:isolation & purification*; D000907:Antibodies, Bacterial / Q000276:immunology; D000918:Antibody Specificity; D001425:Bacterial Outer Membrane Proteins / Q000032:analysis / Q000276:immunology; D001426:Bacterial Proteins; D015153:Blotting, Western; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005516:Food Microbiology; D008562:Membrane Glycoproteins / Q000032:analysis / Q000276:immunology; D012680:Sensitivity and Specificity
D000907:Antibodies, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D008562:Membrane Glycoproteins; C055764:S-layer proteins