Conversion of 2-chloromaleylacetate in Alcaligenes eutrophus JMP134.
2,4-Dichlorophenoxyacetate (2,4-D) in Alcaligenes eutrophus JMP134 (pJP4) is degraded via 2-chloromaleylacetate as an intermediate. The latter compound was found to be reduced by NADH in a maleylacetate reductase catalyzed reaction. Maleylacetate and chloride were formed as products of 2-chloromaleylacetate reduction, the former being funneled into the 3-oxoadipate pathway by a second reductive step. There was no indication for an involvement of a pJP4-encoded enzyme in either the reduction or the dechlorination reaction.
Vollmer
M D
MD
Institut für Mikrobiologie, Universität Stuttgart, Federal Republic of Germany.
Stadler-Fritzsche
K
K
Schlömann
M
M
D015084:2,4-Dichlorophenoxyacetic Acid / Q000378:metabolism*; D000421:Alcaligenes / Q000201:enzymology / Q000378:metabolism*; D001673:Biodegradation, Environmental; D008298:Maleates / Q000378:metabolism*; D010088:Oxidoreductases / Q000378:metabolism*; D044925:Oxidoreductases Acting on CH-CH Group Donors
D008298:Maleates; C011905:maleoylacetic acid; C079154:2-chloromaleylacetate; D015084:2,4-Dichlorophenoxyacetic Acid; D010088:Oxidoreductases; D044925:Oxidoreductases Acting on CH-CH Group Donors; C021530:maleylacetate reductase