Exoerythrocytic development of Plasmodium gallinaceum sporozoites in a chicken fibroblast cell line and inhibition of the cell invasion by specific anti-sporozoite monoclonal antibodies.
Cultivation of the Plasmodium gallinaceum exoerythrocytic forms from sporozoites was attempted in three different cell lines: HEPG2-A16 (from a human hepatoma), VERO (monkey kidney epithelial cells) and SL-29 (chicken embryo fibroblast cells). The sporozoites invaded all three cells types but their development into exoerythrocytic forms occurred only in the SL-29 cells. In the presence of specific monoclonal antibodies against the major circumsporozoite protein, there were varying degrees of inhibition of parasite invasion of the SL-29 cells. Of seven monoclonal antibodies tested, two completely inhibited cell invasion at high concentrations and caused intense inhibition at concentrations as low as 2.5 micrograms/ml, four caused intense inhibition at these various concentrations, and one had no effect on sporozoite invasion.
Rocha
E M
EM
Department of Parasitology, ICB, Federal University of Minas Gerais, Brazil.
Hollingdale
M R
MR
Gwadz
R
R
Krettli
A U
AU
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000913:Antibodies, Protozoan / Q000276:immunology*; D002460:Cell Line; D002478:Cells, Cultured; D002645:Chickens; D002522:Chlorocebus aethiops; D005347:Fibroblasts / Q000469:parasitology; D006801:Humans; D010964:Plasmodium gallinaceum / Q000254:growth & development* / Q000502:physiology; D015800:Protozoan Proteins / Q000276:immunology; D014407:Tumor Cells, Cultured; D014709:Vero Cells
D000911:Antibodies, Monoclonal; D000913:Antibodies, Protozoan; D015800:Protozoan Proteins; C041595:circumsporozoite protein, Protozoan