A yeast antiviral protein, SKI8, shares a repeated amino acid sequence pattern with beta-subunits of G proteins and several other proteins.
SKI8 is a yeast antiviral gene, essential for controlling the propagation of M double-stranded RNA (dsRNA) and thus for preventing virus-induced cytopathology. Our DNA sequence of SKI8 shows that it encodes a 397 amino acid protein containing two copies of a 31 amino acid repeat pattern first identified in mammalian beta-transducin and Cdc4p of yeast. There are also four copies of this repeat in yeast Mak11p, necessary for M dsRNA propagation, and three copies in the putative product of the Dictyostelium AAC3 gene. Analysis of 36 cases of the repeat unit shows they have a consensus predicted structure: N-helix-sheet-turn-sheet-turn-sheet-helix-C.
Matsumoto
Y
Y
Section on Genetics of Simple Eukaryotes, National Institute of Diabetes, Digestive and Kidney Diseases, N.I.H., Bethesda, MD 20892.
Sarkar
G
G
Sommer
S S
SS
Wickner
R B
RB
D000595:Amino Acid Sequence; D001483:Base Sequence; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D005656:Fungal Proteins / Q000737:chemistry* / Q000235:genetics; D019204:GTP-Binding Proteins / Q000737:chemistry; D005800:Genes, Fungal; D016681:Genome, Fungal; D008969:Molecular Sequence Data; D017433:Protein Structure, Secondary; D012091:Repetitive Sequences, Nucleic Acid; D012441:Saccharomyces cerevisiae / Q000737:chemistry* / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins; D017386:Sequence Homology, Amino Acid; D015293:Transducin / Q000737:chemistry*
D004271:DNA, Fungal; D005656:Fungal Proteins; C079882:SKI8 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D019204:GTP-Binding Proteins; D015293:Transducin