Linked functions and the analysis of individual-site binding data.
Linked ligand functions for analyzing experimental data evaluated to yield macroscopic apparent binding constants were well developed by Adair and by Wyman. In this paper, expressions for the linked function (derivative) delta ln(K)/delta ln(c) are applied to both macroscopic association constants and individual-site association constants derived from individual-site binding data. A detailed treatment of a simple interacting system is presented. We demonstrate that data from individual-site isotherms can be interpreted only after explicit assumptions specifying the nature of the ligand-dependent interactions are made.
Saroff
H A
HA
Laboratory of Biochemical Pharmacology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892.
D001665:Binding Sites; D007700:Kinetics; D008024:Ligands; D008956:Models, Chemical; D013816:Thermodynamics
D008024:Ligands