Hepatic and lipoprotein lipases selectively assayed in postheparin plasma.
Sensitive, reliable procedures are reported for the selective assay of lipoprotein lipase (LPL) and hepatic lipase (HL) in postheparin plasma samples. LPL is inhibited in the HL assay by inclusion of 0.76 mol/L sodium chloride in the substrate. In the LPL assay, specificity is attained by pretreating the sample with sodium dodecyl sulfate, which selectively denatures HL. This LPL method was validated by direct comparison with a procedure in which HL is inactivated by an antiserum to human HL. We used the described assays to quantify LPL and HL activity in 32 normal adults, demonstrating a clear sex difference for both enzymes. On average, the men displayed higher HL and lower LPL activities than did the women.
Henderson
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AD
Unit of Metabolic Medicine, St. Mary's Hospital Medical School, Imperial College of Science, Technology and Medicine, London, UK.
Richmond
W
W
Elkeles
R S
RS
D000328:Adult; D053304:Apolipoprotein C-II; D001056:Apolipoproteins C / Q000494:pharmacology; D002250:Carbon Radioisotopes; D004655:Emulsions; D005260:Female; D006170:Gum Arabic; D006493:Heparin; D006801:Humans; D008049:Lipase / Q000037:antagonists & inhibitors / Q000097:blood*; D008071:Lipoprotein Lipase / Q000037:antagonists & inhibitors / Q000097:blood*; D008099:Liver / Q000201:enzymology*; D008297:Male; D010713:Phosphatidylcholines; D011489:Protein Denaturation; D012016:Reference Values; D012588:Scintillation Counting; D012709:Serum Albumin / Q000494:pharmacology; D012965:Sodium Chloride / Q000494:pharmacology; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D014304:Triolein
D053304:Apolipoprotein C-II; D001056:Apolipoproteins C; D002250:Carbon Radioisotopes; D004655:Emulsions; D010713:Phosphatidylcholines; D012709:Serum Albumin; D014304:Triolein; D012967:Sodium Dodecyl Sulfate; D012965:Sodium Chloride; D006170:Gum Arabic; D006493:Heparin; D008049:Lipase; D008071:Lipoprotein Lipase