Cloning and expression of cDNA encoding human betacellulin, a new member of the EGF family.
Betacellulin (BTC) is a novel growth factor purified from the conditioned media of mouse pancreatic beta tumor cells and has been found to be a new member of the epidermal growth factor (EGF) family. The cDNA encoding human BTC has been cloned from a cDNA library prepared from human breast adenocarcinoma cell line MCF-7. The nucleotide sequence encodes a polypeptide which consists of 178 amino acid residues including a putative signal sequence, indicating that the structural organization of human BTC is similar to that of mouse BTC. The amino acid sequence of the human BTC precursor protein exhibits 79% similarity with that of the mouse precursor protein. The BTC gene was found to be expressed in several mouse tissues including kidney and liver as well as in a mouse beta tumor cell line and MCF-7 cells, suggesting that BTC might play a physiological role in normal tissues.
Sasada
R
R
Discovery Research Laboratories II, Takeda Chemical Industries, Ltd., Osaka, Japan.
Ono
Y
Y
Taniyama
Y
Y
Shing
Y
Y
Folkman
J
J
Igarashi
K
K
D000595:Amino Acid Sequence; D001483:Base Sequence; D066259:Betacellulin; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D015870:Gene Expression; D006133:Growth Substances / Q000235:genetics*; D006801:Humans; D066298:In Vitro Techniques; D036341:Intercellular Signaling Peptides and Proteins; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics; D016415:Sequence Alignment; D014162:Transfection; D014407:Tumor Cells, Cultured
C587851:BTC protein, human; D066259:Betacellulin; C587852:Btc protein, mouse; D006133:Growth Substances; D036341:Intercellular Signaling Peptides and Proteins; D012333:RNA, Messenger; D004247:DNA