Proliferative response of Tax1-transduced primary human T cells to anti-CD3 antibody stimulation by an interleukin-2-independent pathway.
The growth properties of human T-cell leukemia virus Tax1-transduced primary human T cells derived from peripheral blood lymphocytes were compared with those of the same subset of T cells transduced with a control vector. Tax1-transduced T cells exhibited slightly elevated responsiveness to externally added interleukin-2 (IL-2) and a markedly higher proliferative response to stimulation with anti-CD3 antibody. The proliferation after anti-CD3 antibody stimulation was mainly via an IL-2-independent pathway. Therefore, some other mechanism than the previously proposed IL-2 autocrine model seems to be involved in the process of deregulation of T-cell proliferation by Tax1. Moreover, Tax1-transduced T cells have continued to proliferate in medium containing IL-2 long after control T cells ceased to grow, and so they are considered to be immortalized.
Akagi
T
T
Virology Division, National Cancer Center Research Institute, Tokyo, Japan.
Shimotohno
K
K
D017252:CD3 Complex / Q000276:immunology*; D002478:Cells, Cultured; D016356:Gene Products, tax / Q000235:genetics / Q000276:immunology*; D005822:Genetic Vectors; D015368:Human T-lymphotropic virus 1 / Q000276:immunology*; D006801:Humans; D007376:Interleukin-2 / Q000378:metabolism; D007377:Interleukin-3 / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D015759:Ionomycin / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects / Q000276:immunology*; D015375:Receptors, Interleukin-2 / Q000276:immunology; D015398:Signal Transduction / Q000187:drug effects / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014161:Transduction, Genetic
D017252:CD3 Complex; D016356:Gene Products, tax; D007376:Interleukin-2; D007377:Interleukin-3; D015375:Receptors, Interleukin-2; D015847:Interleukin-4; D015759:Ionomycin; D013755:Tetradecanoylphorbol Acetate