The human gamma interferon receptor accessory factor encoded by chromosome 21 transduces the signal for the induction of 2',5'-oligoadenylate-synthetase, resistance to virus cytopathic effect, and major histocompatibility complex class I antigens.
Mouse fibroblasts, and human-mouse hybrid fibroblasts carrying only human chromosome 21, were transfected with cDNA encoding full-length human gamma interferon (IFN-gamma) receptor or chimeric IFN-gamma receptor (extracellular domain of the human receptor; transmembrane and intracellular domains of mouse origin). These transfected mouse cells were sensitive to human IFN-gamma only when human chromosome 21 was present. These results show that the species-specific accessory protein encoded by human chromosome 21 interacts with the extracellular domain of human IFN-gamma receptor and transduces the IFN-gamma signal not only for up-regulation of mouse major histocompatibility complex class I antigen expression but also for the induction of 2',5'-oligoadenylate-synthetase and resistance to virus cytopathic effect.
Kalina
U
U
Pharmaceutical Research New Technologies, Hoffmann-La Roche Ltd., Basel, Switzerland.
Ozmen
L
L
Di Padova
K
K
Gentz
R
R
Garotta
G
G
D015088:2',5'-Oligoadenylate Synthetase / Q000096:biosynthesis; D000818:Animals; D002891:Chromosomes, Human, Pair 21; D003588:Cytopathogenic Effect, Viral; D004790:Enzyme Induction; D005805:Genes, MHC Class I; D006801:Humans; D006822:Hybrid Cells; D007371:Interferon-gamma / Q000378:metabolism*; D008285:Major Histocompatibility Complex / Q000276:immunology; D051379:Mice; D017471:Receptors, Interferon / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D015854:Up-Regulation
D017471:Receptors, Interferon; D007371:Interferon-gamma; D015088:2',5'-Oligoadenylate Synthetase