Interferon-alpha 2b inhibits proliferation of human Tenon's capsule fibroblasts.
To evaluate the potential of interferon-alpha 2b (IFN-alpha) as an antifibrotic treatment after glaucoma filtering surgery, we studied its ability to inhibit mitogen-induced proliferation of human Tenon's capsule fibroblasts in vitro, as shown by [3H]-thymidine incorporation. IFN-alpha inhibited fetal calf serum-induced proliferation in a dose-dependent manner in concentrations ranging from 10 to 10,000 IU/ml (P < 0.001). Inhibition was also found when IFN-alpha was added prior to the mitogen (P = 0.001); this inhibitory effect lasted at least 3 days. We suggest that IFN-alpha deserves further evaluation as a possible agent for the control of ocular fibrosis.
Gillies
M
M
Department of Ophthalmology, Prince of Wales Hospital, Randwick, NSW, Australia.
Su
T
T
Sarossy
M
M
Hollows
F
F
D002455:Cell Division; D002478:Cells, Cultured; D003239:Connective Tissue Cells; D004261:DNA Replication; D005347:Fibroblasts / Q000502:physiology*; D006801:Humans; D000077190:Interferon alpha-2; D016898:Interferon-alpha / Q000494:pharmacology*; D011994:Recombinant Proteins
D000077190:Interferon alpha-2; D016898:Interferon-alpha; D011994:Recombinant Proteins