GAP-43 mRNA expression in facial motoneurons during regeneration: in situ hybridization histochemistry study using an alkaline phosphatase-labelled probe.
By means of in situ hybridization histochemistry using an alkaline phosphatase-labelled probe, we found an increase of mRNA for the growth-associated protein GAP-43 in rat facial motoneurons following axotomy of the facial nerve. After nerve resection, the increased level of GAP-43 mRNA was maintained for at least 8 weeks, while it returned to almost undetectable levels by 8 weeks after nerve crush injury. Thus, expression of GAP-43 mRNA in motor neurons paralleled the process of axonal regeneration. However, the increase of GAP-43 mRNA after resection was more pronounced than after crushing, in this way being different from the pattern of low-affinity nerve growth factor receptor mRNA expression.
Saika
T
T
Department of Otolaryngology, Osaka University Medical School, Japan.
Kiyama
H
H
Tohyama
M
M
Matsunaga
T
T
D000469:Alkaline Phosphatase; D000818:Animals; D005154:Facial Nerve / Q000737:chemistry* / Q000601:surgery / Q000648:ultrastructure; D020220:Facial Nerve Injuries; D019922:GAP-43 Protein; D015870:Gene Expression; D006133:Growth Substances / Q000032:analysis* / Q000235:genetics; D008297:Male; D008562:Membrane Glycoproteins / Q000032:analysis* / Q000235:genetics; D009046:Motor Neurons / Q000737:chemistry* / Q000648:ultrastructure; D009416:Nerve Regeneration / Q000235:genetics; D009419:Nerve Tissue Proteins / Q000032:analysis* / Q000235:genetics; D016900:Neurofilament Proteins / Q000032:analysis* / Q000235:genetics; D009693:Nucleic Acid Hybridization; D010750:Phosphoproteins / Q000032:analysis* / Q000235:genetics; D015347:RNA Probes; D012333:RNA, Messenger / Q000032:analysis* / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar
D019922:GAP-43 Protein; D006133:Growth Substances; D008562:Membrane Glycoproteins; D009419:Nerve Tissue Proteins; D016900:Neurofilament Proteins; D010750:Phosphoproteins; D015347:RNA Probes; D012333:RNA, Messenger; D000469:Alkaline Phosphatase