title: The influence of the swine major histocompatibility genes on antibody and cell-mediated immune responses to immunization with an aromatic-dependent mutant of Salmonella typhimurium.
abstract: Eighty-two major histocompatibility complex (MHC) swine leukocyte antigen (SLA) defined miniature pigs from 16 litters were examined for serum agglutinating antibody titer and O-polysaccharide (O-ps) specific peripheral blood lymphocyte blastogenesis following two parenteral vaccinations with 1 x 10(8) aromatic-dependent (aroA) Salmonella typhimurium and following oral challenge with 1 x 10(12) virulent parent S. typhimurium. Least mean squares analysis allowed separate determinations of the effects of MHC genotype, dam, sire and litter. In most cases only litter significantly influenced both lymphocyte blastogenesis and antibody titer before and after vaccination and following challenge. However, pig SLA haplotype significantly influenced the degree of O-ps specific lymphocyte proliferation six days after the second vaccination (p < 0.004). Lymphocyte proliferation and serum agglutinating antibody response six days after primary vaccination were negatively correlated (r2 = -0.68, p < 0.001). In most cases, "dd" and "gg" homozygous and "dg" heterozygous pigs, having the same MHC class II region, behaved immunologically as a group distinct from the other genotypes.
authors.affiliation: Department of Veterinary Microbiology and Immunology, University of Guelph, Ontario.
authors.forename: J S
authors.initials: JS
authors.lastname: Lumsden
authors.forename: B W
authors.initials: BW
authors.lastname: Kennedy
authors.forename: B A
authors.initials: BA
authors.lastname: Mallard
authors.forename: B N
authors.initials: BN
authors.lastname: Wilkie
mesh_terms: D000372:Agglutination Tests / Q000662:veterinary; D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis; D001428:Bacterial Vaccines / Q000276:immunology*; D005838:Genotype; D007111:Immunity, Cellular / Q000235:genetics; D007117:Immunization, Secondary / Q000662:veterinary; D008213:Lymphocyte Activation; D008285:Major Histocompatibility Complex / Q000235:genetics* / Q000276:immunology; D016296:Mutagenesis; D012486:Salmonella typhimurium / Q000235:genetics / Q000276:immunology*; D013552:Swine; D013556:Swine, Miniature / Q000235:genetics* / Q000276:immunology; D014611:Vaccination / Q000662:veterinary*
chemical_list: D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines