UV irradiation-induced interleukin-1 and basic fibroblast growth factor synthesis and release mediate part of the UV response.
UV irradiated cells release into the culture medium factors that induce, when given to nonirradiated cells, the transcription of several UV-inducible genes (collagenase I, human immunodeficiency virus type 1, metallothionein IIA). We identify here the active factors released from UV-treated HeLa cells, as interleukin 1 alpha and basic fibroblast growth factor. UV irradiation leads to increased mRNA levels for both factors and to their enhanced synthesis. Experiments with the drug suramin, which inhibits growth factor-growth factor receptor interactions and with antibodies directed against interleukin 1 alpha and basic fibroblast growth factor, suggest that growth factors do not only transduce the UV-induced signal to nonirradiated cells but act on the producer cell thus establishing an obligatory growth factor loop for at least part of the UV response.
Krämer
M
M
Kernforschungszentrum Karlsruhe, Institut für Genetik und Toxikologie, Germany.
Sachsenmaier
C
C
Herrlich
P
P
Rahmsdorf
H J
HJ
D000906:Antibodies / Q000276:immunology; D015152:Blotting, Northern; D017364:Collagenases / Q000235:genetics / Q000378:metabolism; D003470:Culture Media; D016222:Fibroblast Growth Factor 2 / Q000096:biosynthesis* / Q000276:immunology; D005786:Gene Expression Regulation / Q000528:radiation effects; D006367:HeLa Cells; D006801:Humans; D007375:Interleukin-1 / Q000096:biosynthesis* / Q000276:immunology; D013498:Suramin / Q000494:pharmacology; D014158:Transcription, Genetic / Q000187:drug effects / Q000528:radiation effects; D014466:Ultraviolet Rays
D000906:Antibodies; D003470:Culture Media; D007375:Interleukin-1; D016222:Fibroblast Growth Factor 2; D013498:Suramin; D017364:Collagenases