title: Human PCK1 encoding phosphoenolpyruvate carboxykinase is located on chromosome 20q13.2.
abstract: Cytoplasmic liver phosphoenolpyruvate carboxykinase (GTP) (PEPCK) catalyzes a rate-limiting step in gluconeogenesis. Primers derived from the rat liver PEPCK sequence were used to amplify a portion of the human liver cDNA and to screen a YAC library of human genomic DNA. The sequences of human and rat PEPCK cDNA differed at 16% of the nucleotides compared (45/291). Analysis of a human/rodent hybrid mapping panel demonstrated concordant segregation of PCK1 with chromosome 20. Fluorescence in situ hybridization with YAC DNA further localized PCK1 to subband 20q13.2.
authors.affiliation: Department of Human Genetics, University of Michigan, Ann Arbor 48109.
authors.forename: H
authors.initials: H
authors.lastname: Yu
authors.forename: R
authors.initials: R
authors.lastname: Thun
authors.forename: S
authors.initials: S
authors.lastname: Chandrasekharappa
authors.forename: J M
authors.initials: JM
authors.lastname: Trent
authors.forename: J
authors.initials: J
authors.lastname: Zhang
authors.forename: M H
authors.initials: MH
authors.lastname: Meisler
mesh_terms: D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D015825:Chromosomes, Fungal; D002890:Chromosomes, Human, Pair 20; D006224:Cricetinae; D004277:DNA, Single-Stranded; D015894:Genome, Human; D015698:Genomic Library; D006801:Humans; D006822:Hybrid Cells; D051379:Mice; D008969:Molecular Sequence Data; D010729:Phosphoenolpyruvate Carboxykinase (GTP) / Q000235:genetics*; D012377:Rodentia
chemical_list: D004277:DNA, Single-Stranded; D010729:Phosphoenolpyruvate Carboxykinase (GTP)