title: A monozygotic twin pregnancy after application of zona rubbing on a frozen-thawed blastocyst.
abstract: Assisted hatching techniques enhance the success rate of implantation of in-vitro fertilized human embryos. We report here the successful transfer of a frozen-thawed human blastocyst on which we applied a non-invasive zona rubbing technique (reduction of the thickness of the zona pellucida by gentle rubbing with a microneedle). The implantation in the uterus led to the delivery of healthy monozygotic twins.
authors.affiliation: IVF Centre, Van Helmont Ziekenhuis, Vilvoorde, Belgium.
authors.forename: M
authors.initials: M
authors.lastname: Nijs
authors.forename: P
authors.initials: P
authors.lastname: Vanderzwalmen
authors.forename: G
authors.initials: G
authors.lastname: Segal-Bertin
authors.forename: L
authors.initials: L
authors.lastname: Geerts
authors.forename: E
authors.initials: E
authors.lastname: Van Roosendaal
authors.forename: L
authors.initials: L
authors.lastname: Segal
authors.forename: A
authors.initials: A
authors.lastname: Schoysman-Deboeck
authors.forename: R
authors.initials: R
authors.lastname: Schoysman
mesh_terms: D001755:Blastocyst; D015925:Cryopreservation; D004624:Embryo Transfer / Q000379:methods*; D005260:Female; D006358:Hot Temperature; D006801:Humans; D008297:Male; D008846:Micromanipulation / Q000379:methods*; D011247:Pregnancy; D011272:Pregnancy, Multiple; D014430:Twins, Monozygotic; D015044:Zona Pellucida / Q000502:physiology*