title: Determination of 5,10,15,20-tetra-(m-hydroxyphenyl)chlorin in human plasma by high performance liquid chromatography.
abstract: A high performance liquid chromatographic method for the determination of the photodynamic chemotherapeutic agent 5,10,15,20-tetra(m-hydroxyphenyl)chlorin (m-THPC) in human plasma following intravenous infusion is described. The procedure involves extraction of the drug in plasma with methanol/dimethyl sulphoxide (4:1 v/v) containing 5,10,15,20-tetra(p-hydroxyphenyl)chlorin as the internal standard and separation on a C18 reversed phase column with acetonitrile:0.1% trifluoroacetic acid (77:23 v/v) as the mobile phase. The drug was detected specifically and sensitively at its absorption maximum of 423 nm with a detection limit of 15 ng/mL (signal-to-noise ratio of 5). The intra- and inter-assay coefficients of variation (CV) on analysis of a plasma spiked with m-THPC (1 micrograms/mL) were 2.3 and 3.4% (n = 6), respectively.
authors.affiliation: MRC Toxicology Unit, Medical Research Council Laboratory, Carshalton, Surrey, UK.
authors.forename: Q
authors.initials: Q
authors.lastname: Wang
authors.forename: H B
authors.initials: HB
authors.lastname: Ris
authors.forename: H J
authors.initials: HJ
authors.lastname: Altermatt
authors.forename: B
authors.initials: B
authors.lastname: Reynolds
authors.forename: J C
authors.initials: JC
authors.lastname: Stewart
authors.forename: R
authors.initials: R
authors.lastname: Bonnett
authors.forename: C K
authors.initials: CK
authors.lastname: Lim
mesh_terms: D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007202:Indicators and Reagents; D007262:Infusions, Intravenous; D008652:Mesoporphyrins / Q000008:administration & dosage / Q000097:blood*; D008654:Mesothelioma / Q000097:blood; D012015:Reference Standards; D012997:Solvents
chemical_list: D000970:Antineoplastic Agents; D007202:Indicators and Reagents; D008652:Mesoporphyrins; D012997:Solvents; C072269:temoporfin