A time-resolved fluoroimmunoassay for recombinant human interleukin-3.
We describe a time-resolved fluoroimmunoassay (TRFIA) for recombinant human interleukin-3 (IL-3). The assay design uses two different monoclonal anti-IL-3 antibodies, giving a two-site immunometric assay. The TRFIA for IL-3 is a direct adaptation from our existing ELISA for IL-3. By using the same assay configuration and exchanging a europium-streptavidin conjugate for alkaline phosphatase-streptavidin, we were able to minimize the time required to develop a TRFIA, but still increase the working range of the TRFIA over the ELISA by 20-fold. The limit of detection is 5 ng/L, with the limits of quantification set at 5 and 30,000 ng/L human plasma.
Knopf
H P
HP
Department of Biopharmaceutics, Sandoz Pharma Ltd., Basel, Switzerland.
Duffy
F
F
Papoian
R
R
D004797:Enzyme-Linked Immunosorbent Assay; D015200:Fluoroimmunoassay; D006801:Humans; D007377:Interleukin-3 / Q000097:blood*; D011994:Recombinant Proteins / Q000097:blood
D007377:Interleukin-3; D011994:Recombinant Proteins