title: Uptake and retention of estramustine and the presence of estramustine binding protein in malignant brain tumours in humans.
abstract: Estraumustine phosphate (EMP), a cytotoxic drug used in the treatment of prostatic carcinoma, has been shown to exert cytotoxic effects on glioma cells in vitro. The drug uptake is assumed to depend on a specific estramustine binding protein (EMBP). One of the main difficulties in achieving cytotoxic effect in malignant brain tumours is believed to be due to the poor penetration of cytotoxic drugs into tumour tissue. In patients with malignant supratentorial brain tumours we have analysed the uptake of EMP metabolites in tumour tissue after oral administration and demonstrated EMBP in the same tissue specimens. Sixteen patients were given 280 mg EMP orally 14 h prior to surgery. Specimens from brain tumour tissue, cystic fluid, and serum were collected during surgery. Using gas chromatography the metabolites of EMP, estramustine (EaM) and estromustine (EoM), were quantified, EMBP was demonstrated by immunohistochemistry. The mean concentrations of EaM and EoM, expressed in ng g-1, were 60.3 and 38.4 in tumour tissue and 3.5 and 56.3 in serum, respectively. An accumulation of EaM in tumour tissue was found with a mean concentration gradient of 16.1 versus serum, while the gradient for EoM was 0.76. EMBP was demonstrated with a high degree of staining in all but one tumour. The high concentrations of EaM and EoM found in malignant brain tumour tissue correspond to potentially cytotoxic levels. The present results as well as the earlier in vitro demonstrated cytotoxic effects on glioma cells strengthen the possibility of a therapeutic effect of EMP in the treatment of malignant brain tumours.
authors.affiliation: Department of Oncology, University Hospital, Umeå, Sweden.
authors.forename: A T
authors.initials: AT
authors.lastname: Bergenheim
authors.forename: P O
authors.initials: PO
authors.lastname: Gunnarsson
authors.forename: K
authors.initials: K
authors.lastname: Edman
authors.forename: E
authors.initials: E
authors.lastname: von Schoultz
authors.forename: M I
authors.initials: MI
authors.lastname: Hariz
authors.forename: R
authors.initials: R
authors.lastname: Henriksson
mesh_terms: D000328:Adult; D000368:Aged; D001254:Astrocytoma / Q000097:blood / Q000378:metabolism; D001932:Brain Neoplasms / Q000097:blood / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism*; D004806:Ependymoma / Q000097:blood / Q000378:metabolism; D004961:Estramustine / Q000097:blood / Q000378:metabolism / Q000493:pharmacokinetics*; D004970:Estrone / Q000031:analogs & derivatives / Q000097:blood / Q000378:metabolism; D005260:Female; D005910:Glioma / Q000097:blood / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009588:Nitrogen Mustard Compounds / Q000097:blood / Q000378:metabolism; D029584:Prostatic Secretory Proteins
chemical_list: D002352:Carrier Proteins; D009588:Nitrogen Mustard Compounds; D029584:Prostatic Secretory Proteins; C028321:estramustine-binding protein; D004970:Estrone; D004961:Estramustine; C012115:Leo 271 f