A 5 kDa protein (SCS23) from the 30 S subunit of the spinach chloroplast ribosome.
The proteins of the 30 S ribosomal subunits from spinach chloroplasts were investigated using a radical-free and highly reducing (RFHR) method of two-dimensional polyacrylamide gel electrophoresis (PAGE). Twenty-three proteins were resolved on the gel down to the smallest protein of 5 kDa. The N-terminal amino acid sequence of the 5 kDa protein showed no homology with that of any other protein stored in databases, and the copy numbers were estimated to be 0.88 +/- 0.16 and 0.72 +/- 0.04 in the 30 S subunits and the 70 S ribosomes, respectively. The results suggest that the 5 kDa protein, which we have called SCS23, may be an essential ribosomal protein specific to spinach chloroplasts.
Wada
A
A
Department of Physics, Faculty of Science, Kyoto University, Japan.
Koyama
K
K
Maki
Y
Y
Shimoi
Y
Y
Tanaka
A
A
Tsuji
H
H
D000595:Amino Acid Sequence; D002736:Chloroplasts / Q000737:chemistry* / Q000648:ultrastructure; D004591:Electrophoresis, Polyacrylamide Gel; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010940:Plant Proteins / Q000737:chemistry* / Q000302:isolation & purification; D010944:Plants / Q000648:ultrastructure*; D012269:Ribosomal Proteins / Q000737:chemistry* / Q000302:isolation & purification; D012270:Ribosomes / Q000737:chemistry*; D017386:Sequence Homology, Amino Acid
D010940:Plant Proteins; D012269:Ribosomal Proteins; C040469:ribosomal protein S30