Cloning of alternately spliced mRNA transcripts coding for variants of ovine testicular follitropin receptor lacking the G protein coupling domains.
We report the cloning and characterization of two alternately spliced forms of ovine testicular follitropin receptor mRNA. A smaller receptor cDNA (151 A1) of 727 bp codes for a possible soluble receptor protein of 134 amino acids arising from exons 1-4 of the full length receptor. The 1.1 Kb cDNA clone (HK 18) extending up to the 8th exon codes for a mature protein of 259 amino acids with a single membrane spanning domain predicted by hydropathy analysis. As these structures account for 34% and 61% respectively of the extracellular domain of the full length receptor, we suggest that their putative protein products are likely to possess moderate or high affinity binding sites of physiological significance.
Khan
H
H
Reproduction Research Laboratory, Clinical Research Institute of Montreal, Quebec, Canada.
Yarney
T A
TA
Sairam
M R
MR
D017398:Alternative Splicing; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D005796:Genes; D007438:Introns; D008297:Male; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D051381:Rats; D011962:Receptors, FSH / Q000235:genetics*; D015183:Restriction Mapping; D016415:Sequence Alignment; D012756:Sheep; D013737:Testis / Q000502:physiology
D009838:Oligodeoxyribonucleotides; D011962:Receptors, FSH