title: Telomere directed fragmentation of mammalian chromosomes.
abstract: Cloned human telomeric DNA can integrate into mammalian chromosomes and seed the formation of new telomeres. This process occurs efficiently in three established human cell lines and in a mouse embryonic stem cell line. The newly seeded telomeres appear to be healed by telomerase. The seeding of new telomeres by cloned telomeric DNA is either undetectable or very inefficient in non-tumourigenic mouse or human somatic cell lines. The cytogenetic consequences of the seeding of new telomeres include large chromosome truncations but most of the telomere seeding events occur close to the pre-existing ends of natural chromosomes.
authors.affiliation: Biochemistry Department, Oxford University, UK.
authors.forename: M A
authors.initials: MA
authors.lastname: Barnett
authors.forename: V J
authors.initials: VJ
authors.lastname: Buckle
authors.forename: E P
authors.initials: EP
authors.lastname: Evans
authors.forename: A C
authors.initials: AC
authors.lastname: Porter
authors.forename: D
authors.initials: D
authors.lastname: Rout
authors.forename: A G
authors.initials: AG
authors.lastname: Smith
authors.forename: W R
authors.initials: WR
authors.lastname: Brown
mesh_terms: D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002875:Chromosomes / Q000378:metabolism* / Q000648:ultrastructure; D002877:Chromosomes, Human / Q000378:metabolism* / Q000648:ultrastructure; D003001:Cloning, Molecular; D004247:DNA; D006367:HeLa Cells; D006801:Humans; D017403:In Situ Hybridization; D007621:Karyotyping; D051379:Mice; D008969:Molecular Sequence Data; D016615:Telomere
chemical_list: D004247:DNA