Typing of Clostridium difficile by western immunoblotting with 10 different antisera.
Western blotting (immunoblotting) with antisera against each of 10 reference serogroups was evaluated as a means of typing Clostridium difficile. A total of 164 clinical isolates of C. difficile were tested. Variations in band profiles in each serogroup were used to type isolates into subserogroups. This technique was useful for an epidemiological investigation.
Kato
H
H
Nosocomial Pathogens Laboratory Branch, Centers for Disease Control, Atlanta, Georgia 30333.
Cavallaro
J J
JJ
Kato
N
N
Bartley
S L
SL
Killgore
G E
GE
Watanabe
K
K
Ueno
K
K
D000907:Antibodies, Bacterial; D015373:Bacterial Typing Techniques / Q000706:statistics & numerical data; D015153:Blotting, Western / Q000379:methods* / Q000706:statistics & numerical data; D016360:Clostridioides difficile / Q000145:classification* / Q000276:immunology / Q000302:isolation & purification; D003015:Clostridium Infections / Q000453:epidemiology / Q000382:microbiology; D004812:Epidemiologic Methods; D005069:Evaluation Studies as Topic; D006801:Humans; D012680:Sensitivity and Specificity; D012703:Serotyping
D000907:Antibodies, Bacterial