Fe2+ ions and reduced glutathione--chemical activators of psoralen-sensitized photohaemolysis.
The influence of Fe2+ ions and reduced glutathione (GSH) on the haemolysis of erythrocytes photosensitized (366 nm) by psoralen (PUVA haemolysis) was investigated. PUVA haemolysis was induced by the low fluence rate (24 W m-2) and high fluence (greater than 150 W m-2) of UV-A irradiation. It has been shown that Fe2+ ions and GSH activated PUVA haemolysis at both fluence rates of irradiation. PUVA haemolysis activation by Fe2+ ions was more pronounced than that by GSH. It is supposed that activation caused by Fe2+ ions and GSH is connected with their ability to reduce lipid peroxides or psoralen peroxides with the subsequent formation of free radicals. The regeneration of endogenous Fe2+ by reduced glutathione is also possible.
Potapenko AYa
Department of Medical and Biological Physics, Russian State Medical University, Moscow.
Saparov
S M
SM
Agamalieva
M A
MA
Lysenko
E P
EP
Bezdetnaya
L N
LN
Sukhorukov
V L
VL
D004307:Dose-Response Relationship, Radiation; D004912:Erythrocytes / Q000187:drug effects* / Q000502:physiology; D005296:Ferrous Compounds / Q000494:pharmacology*; D011564:Furocoumarins / Q000494:pharmacology*; D005978:Glutathione / Q000494:pharmacology*; D006461:Hemolysis / Q000187:drug effects* / Q000528:radiation effects; D006801:Humans; D007700:Kinetics; D010777:Photochemistry; D013997:Time Factors; D014466:Ultraviolet Rays
D005296:Ferrous Compounds; D011564:Furocoumarins; C020748:ferrous sulfate; D005978:Glutathione