title: Crystallization and preliminary crystallographic data for formyltetrahydrofolate synthetase from Clostridium thermoaceticum.
abstract: Formyltetrahydrofolate synthetase from Clostridium thermoaceticum has been crystallized using the hanging-drop method from ammonium sulphate and PEG solutions. Crystals are trigonal, the space group is R32, a = 163 A, c = 259 A. A 4.2 A resolution data set has been collected. Analysis of the data using the self-rotation function shows that tetramers have approximate 222 symmetry and are positioned on a crystallographic 2-fold axis.
authors.affiliation: Department of Chemistry and Biochemistry, University of South Carolina, Columbia 29208.
authors.forename: K
authors.initials: K
authors.lastname: Lewinski
authors.forename: Y
authors.initials: Y
authors.lastname: Hui
authors.forename: C G
authors.initials: CG
authors.lastname: Jakob
authors.forename: C R
authors.initials: CR
authors.lastname: Lovell
authors.forename: L
authors.initials: L
authors.lastname: Lebioda
mesh_terms: D003013:Clostridium / Q000201:enzymology*; D003460:Crystallization; D005574:Formate-Tetrahydrofolate Ligase / Q000737:chemistry*; D014961:X-Ray Diffraction
chemical_list: D005574:Formate-Tetrahydrofolate Ligase