Determination of ephedrines in urine by high-performance liquid chromatography.
An improved high-performance liquid chromatographic method with ultraviolet detection for the simultaneous determination of norephedrine, norpseudoephedrine, ephedrine, pseudoephedrine, methylephedrine and ethylephedrine in urine is described. The six substances were separated on a reversed-phase column with phosphate buffer-triethylamine (pH 5.5) as the mobile phase. The linearity and reproducibility were satisfactory for the levels usually found in urine (1-30 micrograms/ml).
Imaz
C
C
Laboratorio de Control del Dopaje, ICEF y D, Consejo Superior de Deportes, Madrid, Spain.
Carreras
D
D
Navajas
R
R
Rodriguez
C
C
Rodriguez
A F
AF
Maynar
J
J
Cortes
R
R
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004809:Ephedrine / Q000031:analogs & derivatives / Q000652:urine*; D006801:Humans; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet
D004809:Ephedrine