title: Specificity of satellite activation by tobacco necrosis virus correlates with nucleic acid hybridization pattern between helper virus isolates.
abstract: Tobacco necrosis virus (TNV) comprises over 20 different isolates which are usually classified on the basis of serological cross-reactivity of their virus particles or specific activation of satellite virus strains (STNV-1, -2, and -C). We have studied the relationships between five TNV isolates, TNV-A, -G, -CN, -D, and -AC36 which exhibit considerable differences in symptom formation on Phaseolus vulgaris. It is shown that, like TNV-A, TNV-G and -CN support the multiplication of STNV-1 and -2. The ability to activate STNV-1 and -2 is not completely correlated with the virulence of the TNV isolates on Phaseolus as TNV-CN infects Phaseolus very inefficiently. The RNAs of all STNV-1 and -2 supporting TNV isolates were detectable by Northern blot analysis using RNA probes derived from TNV-A, whereas the RNAs of the STNV-C activating isolates (TNV-D and -AC36) were only detected with a TNV-D-derived RNA probe. This indicates that the classification of the TNV isolates on the basis of satellite activation is representative of the evolutionary relationships between the isolates.
authors.affiliation: Laboratorium voor Genetica, Universiteit Gent, Belgium.
authors.forename: F
authors.initials: F
authors.lastname: Meulewaeter
authors.forename: X
authors.initials: X
authors.lastname: Danthinne
authors.forename: R
authors.initials: R
authors.lastname: Coutts
authors.forename: J
authors.initials: J
authors.lastname: van Emmelo
mesh_terms: D001483:Base Sequence; D007887:Fabaceae / Q000382:microbiology; D016679:Genome, Viral; D006378:Helper Viruses / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D009693:Nucleic Acid Hybridization; D010942:Plant Viruses / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D010946:Plants, Medicinal; D010947:Plants, Toxic; D015347:RNA Probes; D012367:RNA, Viral / Q000302:isolation & purification; D014026:Nicotiana / Q000382:microbiology; D014775:Virus Activation
chemical_list: D015347:RNA Probes; D012367:RNA, Viral