A novel mutation within the kinase domain of v-abl gene responsible for temperature-sensitive colony-forming ability in soft agar.
Mutation sites of unique temperature-sensitive (ts) mutants of Abelson murine leukemia virus(A-MuLV) that exhibited the ts phenotype in colony-forming ability in soft agar, but not in morphological transformation, were determined. Cloning and sequencing analysis of the full viral genomes of five independent ts mutants revealed a total of 10 mutation sites: 3 mutations (bp714, 742, and 817) located in the 5' untranslated region between LTR and gag gene; 4 mutations (bp1227, 1229, 1512, and 1634) in the gag gene; 3 mutations (point mutations at bp2764 and 3265, and 2-base deletion of bp3448 and 3449) in the abl gene. To determine the mutation sites critical for the ts phenotype in colony-forming ability in soft agar, hybrid viruses were constructed by exchanging the corresponding restriction fragments between wild-type and ts A-MuLV. The hybrid virus containing only the point mutation (T-->G) at bp2764 that exchanged leucine with arginine exhibited the ts phenotype in colony-forming ability in soft agar. Thus, a novel mutation within the kinase domain of the abl gene critical for the ts phenotype in colony-forming ability in soft agar was determined here.
Soma
T
T
Osaka University Medical School, Department of Medicine III, Japan.
Tsukada
S
S
Oka
Y
Y
Kishimoto
T
T
Sugiyama
H
H
D016475:3T3 Cells; D000011:Abelson murine leukemia virus / Q000235:genetics* / Q000502:physiology*; D000362:Agar; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D016313:Genes, abl; D015750:Genes, gag; D016679:Genome, Viral; D051379:Mice; D009154:Mutation; D016374:Oncogene Proteins v-abl / Q000235:genetics*; D010641:Phenotype; D017354:Point Mutation; D011505:Protein-Tyrosine Kinases / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid; D017384:Sequence Deletion; D013696:Temperature
D016374:Oncogene Proteins v-abl; D000362:Agar; D011505:Protein-Tyrosine Kinases