Influences of chronic tobacco smoke inhalation on aging and oxidant-antioxidant balance in the senescence-accelerated mouse (SAM)-P/2.
We studied the influences of chronic tobacco exposure on aging and oxidant-antioxidant balance in two different strains of mice, hitherto called SAM (senescence-accelerated mice). One is a senescence-prone strain, "SAM-P/2," and another is a senescence-resistant strain, "SAM-R/1." We used 100 male mice--20 young (12 weeks of age) mice and 30 mature (24 weeks of age) mice from each strain. Half of each series were housed in a Hamburg II machine and exposed to tobacco smoke inhalation for five weeks. The result was that fewer of the mature SAM-P/2 survived compared with the mature SAM-R/1 after chronic tobacco inhalation. The grading of senility in the mature SAM-P/2 was also significantly higher than that in the mature SAM-R/1. The reduction of glutathione contents of blood and liver after tobacco exposure in the mature SAM-P/2 was greater than that in the young SAM-P/2 and the mature SAM-R/1. Moreover, oxygen radical generation of total blood cells stimulated by phorbol-myristate-acetate or opsonized zymosan showed a greater increase in the mature SAM-P/2 compared to the young SAM-P/2 and the mature SAM-R/1. These results indicate that the senescence-prone strain (SAM-P/2) was more susceptible to tobacco smoke exposure than the resistant strain (SAM-R/1). The impaired oxidant-antioxidant balance in the SAM-P/2 may therefore contribute to the process of senescence acceleration.
Teramoto
S
S
Department of Geriatrics, Faculty of Medicine, University of Tokyo, Japan.
Fukuchi
Y
Y
Uejima
Y
Y
Teramoto
K
K
Orimo
H
H
D000375:Aging; D000818:Animals; D001835:Body Weight; D005123:Eye / Q000378:metabolism; D005609:Free Radicals / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism*; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D010084:Oxidation-Reduction; D010100:Oxygen / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate; D014028:Tobacco Smoke Pollution / Q000009:adverse effects*; D015054:Zymosan
D005609:Free Radicals; D014028:Tobacco Smoke Pollution; D015054:Zymosan; D005978:Glutathione; D013755:Tetradecanoylphorbol Acetate; D010100:Oxygen