title: Therapeutic effects of simultaneous use of glucose and insulin in ketotic dairy cows.
abstract: A glucose tolerance test was carried out on 8 ketotic dairy cows and 5 healthy dairy cows after injection of 500 ml of 50% glucose solution into the jugular vein. The blood insulin concentration increased from 6.3 microU/ml before glucose injection to 62.5 microU/ml after injection in the healthy group and from 3.0 to 22.9 microU/ml in the ketotic group. Insulin concentration before glucose injection was lower, and its increase after glucose injection was smaller, in the ketotic group. Five hundred milliliters of 50% glucose solution were administered to 15 ketotic cows by intravenous infusion once daily from d 1 to 6, and 200 U of insulin were injected subcutaneously in 8 of these cows from d 2 to 6. In the group administered insulin with glucose, the blood glucose concentration, insulin concentration, and insulin to glucagon ratio (50.5 mg/dl, 6.2 microU/ml, and .09, respectively) on d 6 were significantly higher than those for cows administered glucose only (36.3 mg/dl, 3.0 microU/ml, and .04, respectively). Also, the blood ketone bodies and free fatty acid concentrations on d 6 were significantly lower in the group receiving glucose plus insulin (22.1 microU/ml and .79 meq/L, respectively) than in the group receiving glucose alone (63.8 microU/ml and .98 meq/L, respectively). Thus, the simultaneous use of glucose and insulin is considered to be useful for the treatment of ketosis in dairy cows.
authors.affiliation: Department of Veterinary Preventive Medicine, Nihon University, Fujisawa, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Sakai
authors.forename: T
authors.initials: T
authors.lastname: Hayakawa
authors.forename: M
authors.initials: M
authors.lastname: Hamakawa
authors.forename: K
authors.initials: K
authors.lastname: Ogura
authors.forename: S
authors.initials: S
authors.lastname: Kubo
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D002417:Cattle; D002418:Cattle Diseases / Q000188:drug therapy*; D005230:Fatty Acids, Nonesterified / Q000097:blood; D005260:Female; D005947:Glucose / Q000008:administration & dosage* / Q000627:therapeutic use; D005951:Glucose Tolerance Test / Q000662:veterinary; D007328:Insulin / Q000008:administration & dosage* / Q000097:blood / Q000627:therapeutic use; D007657:Ketone Bodies / Q000097:blood; D007662:Ketosis / Q000188:drug therapy / Q000662:veterinary*; D011247:Pregnancy
chemical_list: D001786:Blood Glucose; D005230:Fatty Acids, Nonesterified; D007328:Insulin; D007657:Ketone Bodies; D005947:Glucose