The effect of synthetic thymulin on cell surface marker expression by avian T-cell precursors.
The effects of the in vitro exposure of avian bone marrow (BM) cells and thymocytes to synthetic thymulin were studied. Two T-cell differentiation markers, PNA binding site and CT-1a expression, were used to examine cell maturation. Enhanced PNA binding to both BM cells and thymocytes resulted following an in vitro thymulin exposure but cell proliferation was not affected. Scatchard analysis supported the conclusion that PNA binding affinity was significantly increased and thus responsible for the observed increase in PNA binding. Flow cytometric analysis suggested that the induced PNA+ thymocyte population may be a different population from the one exhibiting enhanced CT-1a expression following thymulin exposure. Taken together, the observations suggest that cells can express their further differentiation states without undergoing proliferation following in vitro thymulin stimulation.
Chang
W P
WP
Department of Microbiology, Immunology, and Parasitology, Cornell University, Ithaca, NY 14853.
Marsh
J A
JA
D000818:Animals; D000954:Antigens, Surface / Q000096:biosynthesis*; D015415:Biomarkers; D001853:Bone Marrow / Q000187:drug effects*; D002454:Cell Differentiation; D002478:Cells, Cultured; D002645:Chickens; D005260:Female; D005434:Flow Cytometry; D005786:Gene Expression Regulation / Q000187:drug effects; D008297:Male; D011975:Receptors, Mitogen / Q000096:biosynthesis*; D013601:T-Lymphocytes / Q000187:drug effects*; D013935:Thymic Factor, Circulating / Q000138:chemical synthesis / Q000494:pharmacology*
D000954:Antigens, Surface; D015415:Biomarkers; D011975:Receptors, Mitogen; C038223:peanut agglutinin receptor; D013935:Thymic Factor, Circulating