Simple microscale high-performance liquid chromatographic method for determination of furosemide in neonatal plasma.
A simple microscale high-performance liquid chromatographic method using fluorescence detection for the quantitation of furosemide in neonatal plasma is described. Sample pre-treatment involved protein precipitation of 25 microliters of plasma with 100 microliters of acetonitrile. The mobile phase consisted of acetonitrile (460 ml) and 0.08 M orthophosphoric acid (540 ml) and was delivered at 1.1 ml/min. Calibration curves were linear from 0.1 to 25 micrograms/ml. Within-day and between-day imprecision (coefficient of variation) was 3.9-6.1, and 6.1-12.2%, respectively. Furosemide was eluted after 6.5 min and naproxen (internal standard) after 11.5 min. The assay was validated for application in neonatal plasma containing a wide range of albumin concentrations.
Sidhu
J S
JS
Department of Pharmacy, University of Queensland, Brisbane, Australia.
Charles
B G
BG
D002851:Chromatography, High Pressure Liquid; D005665:Furosemide / Q000097:blood*; D006801:Humans; D007231:Infant, Newborn; D009288:Naproxen / Q000097:blood; D012015:Reference Standards; D012709:Serum Albumin / Q000032:analysis; D013050:Spectrometry, Fluorescence
D012709:Serum Albumin; D009288:Naproxen; D005665:Furosemide