title: Radiolysis in aqueous solution of dinucleoside monophosphates by high-energy electrons and fission neutrons.
abstract: The radiation chemistry in aqueous solution of the dinucleoside monophosphate d-[CpT] and its sequence isomer d-[TpC] in air or nitrogen was examined using different qualities and quantities of radiations. High-performance liquid chromatography and gas chromatography-mass spectrometry were used to analyze the high-energy electron (13.2 MeV) exposure products or fission-neutron exposure products of d-[CpT] and d-[TpC]. A comparison of product profiles obtained from irradiated d-[CpT] and d-[TpC] suggests that, at relatively low radiation doses (50-250 Gy), products are formed by N-glycosidic or phosphodiester bond-cleavage, while at higher doses (500-1000 Gy) additional products were detected as a consequence of ring-modification mechanisms. The plots of radiation dose-yield and corresponding calculated G values of the released undamaged bases and nucleosides from d-[CpT] and d-[TpC] suggest a base-sequence dependence and a quality- and quantity-dependent response to ionizing radiation. Although the product quantities formed from sequence isomers were slightly different, we found no qualitative differences in the product formed at the lowest doses examined.
authors.affiliation: Radiation Biochemistry Department, Armed Forces Radiobiology Research Institute, Bethesda, Maryland 20889-5145.
authors.forename: Y N
authors.initials: YN
authors.lastname: Vaishnav
authors.forename: C E
authors.initials: CE
authors.lastname: Swenberg
mesh_terms: D002851:Chromatography, High Pressure Liquid; D015226:Dinucleoside Phosphates / Q000528:radiation effects*; D004583:Electrons; D008401:Gas Chromatography-Mass Spectrometry; D009502:Neutrons; D012996:Solutions; D014867:Water
chemical_list: D015226:Dinucleoside Phosphates; D012996:Solutions; D014867:Water