title: Adhesion proteins on human microglial cells and modulation of their expression by IL1 alpha and TNF alpha.
abstract: Expression of adhesion proteins on human microglial cells was studied by immunocytochemistry. Both microglial cells and peripheral blood monocytes expressed beta 2 integrins and molecules of the immunoglobulin superfamily at similar levels whereas the expression of the beta 1 integrins (alpha 2-VLA (very late antigen), alpha 4-VLA, alpha 5-VLA, alpha 6-VLA) was higher on microglial cells than on monocytes. Stimulation of microglial cells with interleukin-1 alpha and tumour necrosis factor-alpha, the main cytokines detected in HIV1-infected central nervous system (CNS), increased the microglial expression of alpha 1-VLA, intercellular adhesion molecule-1, vascular cell adhesion molecule-1 and beta 2-LFA-1 (leukocyte-function-associated molecule-1) but not of alpha L-LFA-1. Such an induction of adhesion molecules could facilitate penetration of HIV1-infected monocytes into brain parenchyma and their adhesion to CNS cells, and could maintain a chronic inflammation during human immunodeficiency virus-1 (HIV1) encephalopathy.
authors.affiliation: Laboratoire de Neurovirologie et Neuroimmunologie, Université Paris XI, UFR Kremlin-Bicêtre.
authors.forename: G
authors.initials: G
authors.lastname: Sébire
authors.forename: C
authors.initials: C
authors.lastname: Héry
authors.forename: S
authors.initials: S
authors.lastname: Peudenier
authors.forename: M
authors.initials: M
authors.lastname: Tardieu
mesh_terms: D001921:Brain / Q000166:cytology*; D002478:Cells, Cultured; D006801:Humans; D007136:Immunoglobulins / Q000378:metabolism*; D016023:Integrins / Q000378:metabolism*; D007375:Interleukin-1 / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
chemical_list: D007136:Immunoglobulins; D016023:Integrins; D007375:Interleukin-1; D014409:Tumor Necrosis Factor-alpha