Abscess scintigraphy with 99mTc-human immunoglobulin (IgG) using a one-step labeling method.
It was shown earlier that non-specific human gamma globulin (IgG) labeled with 111In can be used as an agent for abscess localization. We describe experimental results with 99mTc-IgG in animals bearing abscesses and tumors using a one-step labeling method with 99mTc. We studied this compound in several animal models: mice bearing turpentine abscesses and subcutaneously transplanted sarcomas, in rats with turpentine or E. coli abscesses and intracerebrally implanted gliomas and in rabbits with E. coli or turpentine abscesses. Blood clearance was studied in dogs. It was found that the absolute concentration of 111In-IgG in abscess and tumor was higher than that of 99mTc-IgG. However, the abscess-to-tumor ratio was higher for 99mTc-IgG. The 99mTc-IgG images were of high quality and abscesses could be detected as early as 30 min post-injection (p.i.). It appears that 99mTc-IgG has many potential advantages over 111In-IgG because of better physical properties of 99mTc, simpler preparation, lower cost and greater availability and the possibility of using higher 99mTc doses.
Oster
Z H
ZH
Brookhaven National Laboratory, Medical Department, Upton, New York 11973.
Som
P
P
Rhodes
B A
BA
Wong
C T
CT
Cabahug
C
C
Sacker
D F
DF
Wang
G J
GJ
Meinken
G E
GE
D000038:Abscess / Q000139:chemically induced / Q000000981:diagnostic imaging*; D000818:Animals; D004285:Dogs; D004927:Escherichia coli Infections / Q000000981:diagnostic imaging* / Q000378:metabolism; D005260:Female; D005910:Glioma / Q000000981:diagnostic imaging* / Q000378:metabolism; D007074:Immunoglobulin G; D007136:Immunoglobulins / Q000378:metabolism*; D007205:Indium Radioisotopes / Q000493:pharmacokinetics*; D007668:Kidney / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D008657:Metabolic Clearance Rate; D051379:Mice; D008808:Mice, Inbred CBA; D009374:Neoplasms, Experimental / Q000000981:diagnostic imaging* / Q000378:metabolism; D011817:Rabbits; D011877:Radionuclide Imaging; D051381:Rats; D011916:Rats, Inbred F344; D012513:Sarcoma, Experimental / Q000000981:diagnostic imaging* / Q000378:metabolism; D013667:Technetium / Q000493:pharmacokinetics*; D014018:Tissue Distribution; D014425:Turpentine / Q000633:toxicity
D007074:Immunoglobulin G; D007136:Immunoglobulins; D007205:Indium Radioisotopes; C065376:technetium Tc 99m immunoglobulin; D013667:Technetium; D014425:Turpentine