Cultured adult cardiac myocytes maintain protein synthetic capacity of intact adult hearts.
Previous studies have shown that the rates of protein synthesis observed in embryonic and neonatal heart cells in culture are as much as nine times greater than the rates of synthesis observed in the intact adult heart either in situ or in isolated perfusion studies. This study addressed whether adult cardiomyocytes in long-term culture maintain the protein synthetic capacity of the adult myocardium or, rather, whether the protein synthetic capacity expands or falls as adult cardiac myocytes progress in culture. Protein synthesis was evaluated in isolated adult feline cardiomyocytes maintained in serum and insulin-supplemented medium for up to 53 days in vitro. With the use of both pulse- and saturation-labeling techniques it was determined that the rate of protein synthesis in adult cardiomyocytes was maintained at a level very close to that observed in the intact heart for over 1 mo in culture. Saturation-labeling studies indicate a fractional rate of protein synthesis at 6.1%/day and an absolute synthesis rate of 1,300 nmol leucine incorporated.g protein-1.h-1. Pulse-labeling studies revealed an initial increase in protein synthesis rates during adaptation to culture and a further increase after activation of beating and cellular hypertrophy.
Clark
W A
WA
Department of Medicine, Northwestern University Medical School, Chicago, Illinois 60611.
Rudnick
S J
SJ
Simpson
D G
DG
LaPres
J J
JJ
Decker
R S
RS
D000375:Aging / Q000378:metabolism; D000818:Animals; D002415:Cats; D002452:Cell Count; D002478:Cells, Cultured; D006984:Hypertrophy; D007700:Kinetics; D007930:Leucine / Q000378:metabolism; D009124:Muscle Proteins / Q000096:biosynthesis*; D009200:Myocardial Contraction; D009206:Myocardium / Q000166:cytology / Q000378:metabolism* / Q000473:pathology
D009124:Muscle Proteins; D007930:Leucine