Genetic analysis of the bchC and bchA genes of Rhodobacter sphaeroides.
This study has identified by sequence analysis a single gene in the bchC locus of Rhodobacter sphaeroides and three genes, designated bchX, Y and Z, in the bchA locus, which was previously thought to contain only a single gene. All four genes may reside within the same operon and are transcribed in the order bchC-X-Y-Z. Complementation analysis of eight transposon insertion mutants within these genes suggests that bchX, Y and Z are essential for the reduction of 2-devinyl-2-hydroxyethyl chlorophyllide a and that bchC encodes the 2-desacetyl-2-hydroxyethyl bacteriochlorophyllide a dehydrogenase. Similarity between the putative BchX protein and dinitrogenase reductase proteins suggests that BchX may also be a reductase, supplying electrons for reduction of 2-devinyl-2-hydroxyethyl chlorophyllide a.
McGlynn
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Krebs Institute for Biomolecular Research, University of Sheffield, UK.
Hunter
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D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D001429:Bacteriochlorophylls / Q000096:biosynthesis*; D001483:Base Sequence; D005798:Genes, Bacterial / Q000235:genetics*; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000235:genetics*; D012322:RNA Precursors / Q000235:genetics; D012242:Rhodobacter sphaeroides / Q000235:genetics*; D017422:Sequence Analysis, DNA
D001426:Bacterial Proteins; D001429:Bacteriochlorophylls; D012322:RNA Precursors; C026083:bacteriochlorophyll A proteins, Bacteria; D010088:Oxidoreductases; C061969:2-desacetyl-2-hydroxyethyl bacteriochlorophyllide a dehydrogenase