title: Investigations of the gallbladder pathology associated with dietary exposure to disodium arsenate heptahydrate in juvenile rainbow trout (Oncorhynchus mykiss).
abstract: Juvenile rainbow trout were fed semi-purified diets with (58 micrograms As/g diet) or without arsenic, added as disodium arsenate heptahydrate (DSA), under standard laboratory conditions for up to 12 weeks, to determine the time-course of development of gallbladder pathology in response to dietary DSA exposure, to correlate this pathology with levels of total arsenic and specific arsenic metabolites in the hepatobiliary system and thereby to attempt to gain some insight regarding the mechanism(s) by which the pathological changes develop. Gallbladder lesions associated with this level of dietary arsenic exposure to juvenile rainbow trout include acute inflammation with oedema of the submucosal tissues and sloughing of the epithelium within the first day of exposure, developing to chronic inflammation with fibrosis of the gallbladder wall. These changes may result from the toxic influence of arsenite absorbed into the epithelial cells. The arsenic content of washed gallbladder tissue is a sensitive indicator of recent dietary DSA exposure, while the chronic inflammatory lesion with extensive fibrosis of the gallbladder wall may provide a longer-term indicator of exposure to toxic levels of DSA in the diet of rainbow trout.
authors.affiliation: Department of Nutritional Sciences, University of Guelph, Ontario, Canada.
authors.forename: K A
authors.initials: KA
authors.lastname: Cockell
authors.forename: W J
authors.initials: WJ
authors.lastname: Bettger
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001149:Arsenates / Q000378:metabolism / Q000493:pharmacokinetics / Q000633:toxicity*; D001151:Arsenic / Q000378:metabolism / Q000493:pharmacokinetics / Q000633:toxicity*; D001646:Bile / Q000737:chemistry; D001835:Body Weight / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005704:Gallbladder / Q000187:drug effects* / Q000473:pathology*; D014018:Tissue Distribution; D014337:Trout
chemical_list: D001149:Arsenates; C009277:sodium arsenate; D001151:Arsenic