T-cell chemotactic activity of cytokine LD78: a comparative study with interleukin-8, a chemotactic factor for the T-cell CD45RA+ phenotype.
The recombinant LD78 cytokine (rLD78) promoted migration of human peripheral T lymphocytes at concentrations from 10(-11) M to 10(-7) M, with a peak of activity at 10(-8) M. There was no difference in migration promoting activity between LD78 alpha and its variant LD78 beta. Checkerboard analysis indicated that the migration of T cells attracted by rLD78 was chemotaxis but not chemokinesis. The chemotactic activity of rLD78 was apparently ten-fold lower than that of recombinant inteleukin-8 (rIL-8) when peripheral T cells were used as the indicator cells. Subset analysis on T cells responding to rLD78 or rIL-8 indicated that rIL-8 was more chemotactic for the CD45RA+ T cell subset than the CD45RO+ subset, but did not distinguish between CD4+ and CD8+ T cell subsets. In contrast, rLD78 apparently distinguished neither between CD4+ and CD8+ T cell subsets nor between CD45RA+ and CD45RO+ subsets.
Tanaka
J
J
Division of Molecular Pathology, Graduate School of Medical Sciences, Kumamoto University, Japan.
Nomiyama
H
H
Yamamoto
T
T
Hamada
F
F
Kambara
T
T
D002465:Cell Movement / Q000187:drug effects; D054407:Chemokine CCL4; D002634:Chemotaxis, Leukocyte / Q000502:physiology*; D016207:Cytokines / Q000276:immunology*; D006801:Humans; D016209:Interleukin-8 / Q000502:physiology; D017493:Leukocyte Common Antigens / Q000032:analysis / Q000235:genetics; D019402:Macrophage Inflammatory Proteins; D015846:Monokines / Q000276:immunology*; D009363:Neoplasm Proteins / Q000276:immunology*; D010641:Phenotype; D011994:Recombinant Proteins / Q000276:immunology; D016176:T-Lymphocyte Subsets / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology
D054407:Chemokine CCL4; D016207:Cytokines; D016209:Interleukin-8; D019402:Macrophage Inflammatory Proteins; D015846:Monokines; D009363:Neoplasm Proteins; D011994:Recombinant Proteins; D017493:Leukocyte Common Antigens