Sequence analysis of monkey acrosin-trypsin inhibitor transcripts and their abundant expression in the epididymis.
Seven independent cross-hybridizing clones have been isolated from a Macaca fascicularis epididymal cDNA library and their inserts shown to correspond to a secreted inhibitor of the sperm serine proteinase, acrosin. Hybridizing transcripts of approx. 0.6 kb have been shown by Northern blot analysis to be considerably more abundant in the epididymis than the testis. This finding is in surprising contrast to a previous report that human acrosin-trypsin inhibitor mRNA predominates in the testis.
Perry
A C
AC
Department of Biochemistry, University of Bristol, UK.
Jones
R
R
Hall
L
L
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D004822:Epididymis / Q000502:physiology*; D015870:Gene Expression; D006801:Humans; D008252:Macaca fascicularis; D008297:Male; D008969:Molecular Sequence Data; D011506:Proteins; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic; D014361:Trypsin Inhibitors / Q000235:genetics*
D011506:Proteins; D014361:Trypsin Inhibitors; C079955:acrosin-trypsin inhibitor, Macaca fascicularis; D004247:DNA