Changes in Hox1.6, c-jun, and Oct-3 gene expressions are associated with teratocarcinoma F9 cell differentiation in three different ways of induction.
To obtain information on the mechanism of teratocarcinoma cell differentiation, the expression of genes having a specific role in gene regulation was examined using three different methods of inducing differentiation: drug treatments with retinoic acid (RA) and sodium butyrate, and a genetic approach using ts mutants. The RAR-beta and MK genes were induced specifically by RA treatment, which indicates that these two genes are solely RA responsive. By contrast, a change in c-jun, Hox1.6, and Oct-3 gene expression was observed in all three methods of F9 cell differentiation. These findings suggest that these three genes, whether down-or up-regulated, play an important and key role during teratocarcinoma cell differentiation.
Iwai
S A
SA
2nd Department of Oral Surgery, Dental Faculty, Osaka University, Japan.
Kosaka
M
M
Nishina
Y
Y
Sumi
T
T
Sakuda
M
M
Nishimune
Y
Y
D000818:Animals; D002087:Butyrates / Q000494:pharmacology; D020148:Butyric Acid; D002454:Cell Differentiation / Q000235:genetics*; D054278:Embryonal Carcinoma Stem Cells; D015870:Gene Expression / Q000187:drug effects; D016758:Genes, jun / Q000187:drug effects / Q000502:physiology*; D009154:Mutation; D014411:Neoplastic Stem Cells / Q000187:drug effects; D014212:Tretinoin / Q000494:pharmacology
D002087:Butyrates; D020148:Butyric Acid; D014212:Tretinoin