Cell content and albumin concentration in nasal lavage from patients with rhinitis.
Influx of inflammatory cells in the nose was evaluated in 14 patients with rhinitis and 11 healthy subjects. Nasal secretions were obtained by nasal lavages. Total cell count and differential count were carried out with a Neubauer chamber and in cytospin slides, respectively. Cells were classified according to their morphology as epithelial cells without nuclei (degenerated), epithelial nucleated cells, neutrophils, eosinophils, and other. Total protein and albumin concentration were measured by the Lowry method and a nephelometric assay respectively. A statistically significant difference was found in total nucleated epithelial cells (P < .01), total neutrophils (P < .01), and total eosinophils (P < .05) between patients and healthy subjects. A significant correlation was found between albumin/total protein concentration with eosinophil numbers (r = .83, P < .001) in patients with rhinitis. These findings suggest that eosinophils participate in the inflammatory process taking place in rhinitis. Since the cellularity present in the nose of rhinitic patients is similar to that usually found in the bronchial secretions of asthmatic patients obtained by bronchoalveolar lavage, our results also suggest that nasal lavage may be used in the study of the inflammatory cells involved in airway diseases.
Prat
J
J
Servei de Pneumologia, Hospital Clinic, Facultat de Medicina, Barcelona, Spain.
Xaubet
A
A
Mullol
J
J
Plaza
V
V
Picado
C
C
D000328:Adult; D000418:Albumins / Q000032:analysis; D002469:Cell Separation; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009297:Nasal Mucosa / Q000737:chemistry / Q000378:metabolism* / Q000473:pathology; D012220:Rhinitis / Q000378:metabolism* / Q000473:pathology*; D007507:Therapeutic Irrigation
D000418:Albumins