title: Matrix production of smooth muscle cells from rat aorta in vitro.
abstract: Immunofluorescence microscopic methods served to demonstrate the production of the following matrix components in cultures of vascular smooth muscle cells from rat aorta: fibronectin; nidogen; heparan sulphate-proteoglycan (HS-PG); laminin; and collagen types I, III, IV, V, and VI. A time-dependence of synthesis and secretion could be shown for a number of components of the extracellular matrix (ECM), such as laminin. The results revealed the following estimated quantitative differences of the collagen types: type I > type III > types V and VI. A filamentous/fibrillar matrix and also occasionally a typical basal lamina could be demonstrated electron microscopically around the smooth muscle cells.
authors.affiliation: Institute of Anatomy, Free University of Berlin, Germany.
authors.forename: B
authors.initials: B
authors.lastname: Stiemer
authors.forename: G
authors.initials: G
authors.lastname: Springmeier
authors.forename: L
authors.initials: L
authors.lastname: el-Jarad
authors.forename: C
authors.initials: C
authors.lastname: Schröter-Kermani
mesh_terms: D000199:Actins / Q000276:immunology; D000818:Animals; D000917:Antibody Formation; D001011:Aorta / Q000276:immunology / Q000648:ultrastructure*; D003094:Collagen; D005455:Fluorescent Antibody Technique; D007150:Immunohistochemistry; D007797:Laminin / Q000648:ultrastructure; D051379:Mice; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D009131:Muscle, Smooth, Vascular / Q000276:immunology / Q000648:ultrastructure*; D051381:Rats
chemical_list: D000199:Actins; D007797:Laminin; D003094:Collagen