title: The structure of the 5' terminal cap of the respiratory syncytial virus mRNA.
abstract: The 5' terminal cap structure of the mRNAs of human respiratory syncytial virus synthesized in vitro in the presence of S-adenosyl-L-methionine consists of a 7-methyl guanosine linked to an unmethylated guanosine through a 5'-5' pyrophosphate linkage formed by using the alpha and beta phosphates of GTP. The complete cap structure is m7G (5')ppp(5')Gp ... which is devoid of ribose 2'-O-methylation. Capping, including methylation, is coupled to transcription. These results constitute the first report of a pneumoviral mRNA cap structure.
authors.affiliation: Department of Molecular Biology, Cleveland Clinic Foundation, Ohio 44195.
authors.forename: S
authors.initials: S
authors.lastname: Barik
mesh_terms: D001483:Base Sequence; D008969:Molecular Sequence Data; D012315:RNA Caps / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics*; D012136:Respiratory Syncytial Viruses / Q000235:genetics*
chemical_list: D012315:RNA Caps; D012333:RNA, Messenger; D012367:RNA, Viral