Molecular cloning and sequence of the cynomolgus monkey apolipoprotein A-II gene.
A clone containing the coding region for cynomolgus monkey (Macaca fascicularis) apolipoprotein A-II has been isolated from a cynomolgus genomic DNA library. The gene spans 1.4 kilobases (kb). The complete nucleic acid sequence of the apolipoprotein A-II gene has been determined, establishing that the gene is interrupted by three intervening sequences of 170, 273 and 394 bp, respectively. The open reading frame encodes a protein of 100 amino acids, and shows 94% sequence similarity with its human equivalent. Both apolipoproteins have identical signal peptide. A noticeable feature is the substitution of mature human Cys-6 for Ser. This change explains the existence of cynomolgus apolipoprotein A-II as a monomer and may have important consequences in the kinetics of this apolipoprotein.
Osada
J
J
Departmento de Fisiologia, Facultad de Medicina, Universidad de Valencia, Spain.
Garcés
C
C
Sastre
J
J
Schaefer
E J
EJ
Ordovás
J M
JM
D000818:Animals; D016633:Apolipoprotein A-II / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D006801:Humans; D007438:Introns; D008252:Macaca fascicularis / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D012689:Sequence Homology, Nucleic Acid
D016633:Apolipoprotein A-II