Analysis of the distribution of protein binding DNA motives in the vicinity of the 3'-side chicken alpha-globin enhancer.
DNA-protein interactions in the vicinity of the 3'-side chicken alpha-globin enhancer were analyzed in vitro. Downstream of the three GATA-1 motifs which constitute the core enhancer, a 4th homology box, containing two further DNA binding motifs, was found by sequence alignment between duck and chicken. One was identified as being a fourth GATA-1 motif in opposite polarity, while the other contains a putative AP-1/NF-E2 binding site. In addition, it was found that several GGTGG motifs interacting with nuclear factors are interspersed between the different GATA-1 sites. When nuclear extracts from chicken erythroblasts transformed by an AEV ts-mutant (clone HD3) or from adult chicken erythrocytes were compared, different protein factors were found to interact with these DNA motifs in DNase I footprinting and electrophoretic retardation experiments. These results are discussed in terms of a general distribution of protein-binding DNA sequences and the presence of nuclear factors in the majority of globin and also some non-globin gene control regions.
Targa
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Institut Jacques Monod CNRS, Université de Paris VII, France.
de Moura Gallo
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Scherrer
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D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002478:Cells, Cultured; D002645:Chickens; D004268:DNA-Binding Proteins / Q000378:metabolism*; D004742:Enhancer Elements, Genetic; D005796:Genes; D005914:Globins / Q000235:genetics*; D066298:In Vitro Techniques; D008745:Methylation; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D012045:Regulatory Sequences, Nucleic Acid
D004268:DNA-Binding Proteins; D009838:Oligodeoxyribonucleotides; D005914:Globins