title: Proliferation and differentiation of tumour cells from B-cell lymphoma of mucosa-associated lymphoid tissue in vitro.
abstract: Several characteristics of lymphomas of mucosa-associated lymphoid tissue (MALT type) suggest that they are antigen-dependent and that their growth parodies a normal immune response. We have previously shown that three cases of low-grade B-cell MALT-type lymphoma recognize autoantigens. In this study, we investigated the response of three low-grade and one high-grade case of MALT-type lymphoma to anti-idiotypic antibody as a model of antigen binding either alone or as a co-stimulus with B-cell mitogens. We also studied the response of tumour cells to interleukin-6 (IL-6), which induces differentiation to immunoglobulin-producing cells in many systems. Of the four cases studied, one low-grade case showed markedly enhanced proliferation in response to anti-idiotype alone. This could not be increased by the addition of mitogens. In the remaining two low-grade cases, mitogen responsiveness was observed which was affected by anti-idiotype either by an enhancement or by a reduction in the proliferative response. The high-grade case failed to respond to the stimuli studied. No response to IL-6 was observed. This study supports the suggestion that antigen may affect the pathogenesis of low-grade tumours of MALT type.
authors.affiliation: Department of Histopathology, University College and Middlesex School of Medicine, London, U.K.
authors.forename: T
authors.initials: T
authors.lastname: Hussell
authors.forename: P G
authors.initials: PG
authors.lastname: Isaacson
authors.forename: J
authors.initials: J
authors.lastname: Spencer
mesh_terms: D000888:Antibodies, Anti-Idiotypic / Q000378:metabolism*; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000276:immunology; D006801:Humans; D007136:Immunoglobulins / Q000096:biosynthesis; D015850:Interleukin-6 / Q000494:pharmacology; D008221:Lymphoid Tissue / Q000473:pathology; D016393:Lymphoma, B-Cell / Q000276:immunology / Q000473:pathology*; D009092:Mucous Membrane / Q000276:immunology; D014407:Tumor Cells, Cultured / Q000187:drug effects
chemical_list: D000888:Antibodies, Anti-Idiotypic; D007136:Immunoglobulins; D015850:Interleukin-6