Factor VIII:c concentrate virus inactivated: progress in purification by using classic chromatographic methods.
This study was carried out with the aim of developing a production process for the manufacture of a highly purified factor VIII concentrate which is virus inactivated by pasteurization in liquid phase. Beside standard plasma protein separation techniques, the procedure uses a chromatographic step on anion exchanger, whose selectivity is increased by using high, but not destabilizing salt concentrations. The final product before stabilization has a specific activity higher than 300 IU/mg of protein, namely the highest specific activity reported for human factor VIII concentrates purified without the use of immunoadsorbents.
Arrighi
S
S
Sclavo Biotechnology Center, Siena, Italy.
Pacenti
L
L
Borri
M G
MG
D002852:Chromatography, Ion Exchange / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D005169:Factor VIII / Q000302:isolation & purification*; D006358:Hot Temperature; D006801:Humans; D014775:Virus Activation
D005169:Factor VIII