Measurement of terbutaline and salbutamol in plasma by high performance liquid chromatography with fluorescence detection.
A method is described for the determination of terbutaline and salbutamol in plasma from patients given maximal therapy for brittle asthma. The analytes were isolated by solid phase extraction on alkali-treated Bond-Elut, unmodified, silica columns and measured by high performance liquid chromatography with fluorescence detection (excitation wavelength 200 nm). The limits of detection for a 1 mL sample containing salbutamol and terbutaline were 1 microgram/L and 2.5 micrograms/L, respectively. The intra-assay precision (CV) for samples containing 25 micrograms/L was 3.6 and 5.0% respectively. This method was applied to the measurement of terbutaline in samples from patients given continuous infusions of the drug to assess whether this treatment might result in toxicity.
McCarthy
P T
PT
National Poisons Unit, Guys and Lewisham NHS Trust, London, UK.
Atwal
S
S
Sykes
A P
AP
Ayres
J G
JG
D000420:Albuterol / Q000097:blood* / Q000493:pharmacokinetics / Q000627:therapeutic use; D001249:Asthma / Q000097:blood / Q000188:drug therapy; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007202:Indicators and Reagents; D007279:Injections, Subcutaneous; D013050:Spectrometry, Fluorescence; D013726:Terbutaline / Q000097:blood* / Q000493:pharmacokinetics / Q000627:therapeutic use
D007202:Indicators and Reagents; D013726:Terbutaline; D000420:Albuterol