N.m.r. lipid profiles of cells, tissues and body fluids. Neutral, non-acidic and acidic phospholipid analysis of Bond Elut chromatographic fractions.
Although the advantages of in vitro n.m.r. analysis of cellular lipids have been documented, this purely n.m.r. approach also has drawbacks. Rapid and quantitative separation of total lipids into neutral lipids, non-esterified fatty acids, non-acidic phospholipids and acidic phospholipids using Bond Elut ion-exchange columns as demonstrated here permitted a more quantitative and complete n.m.r. analysis of glycerides, cholesterol, saturated and unsaturated sphingolipids and ether lipids, as well as of diacylcholine and ethanolamine lipids. Acidic lipids were also analysed. The fatty acid compositions of the intact lipids in each of the four Bond Elut fractions were determined from the n.m.r. spectrum of each fraction.
Choi
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Department of Pharmaceutical Chemistry, School of Pharmacy, University of London, U.K.
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Gibbons
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D000818:Animals; D001826:Body Fluids / Q000737:chemistry*; D002477:Cells / Q000737:chemistry*; D002784:Cholesterol / Q000032:analysis; D002794:Choline / Q000032:analysis; D002852:Chromatography, Ion Exchange; D019856:Ethanolamine; D004983:Ethanolamines / Q000032:analysis; D005230:Fatty Acids, Nonesterified / Q000032:analysis; D005231:Fatty Acids, Unsaturated / Q000032:analysis; D005989:Glycerides / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy; D010743:Phospholipids / Q000032:analysis*; D051381:Rats; D013107:Sphingolipids / Q000032:analysis
D004983:Ethanolamines; D005230:Fatty Acids, Nonesterified; D005231:Fatty Acids, Unsaturated; D005989:Glycerides; D010743:Phospholipids; D013107:Sphingolipids; D019856:Ethanolamine; D002784:Cholesterol; D002794:Choline