Isolation of a dsRNA virus from Dipodascus (Endomyces) magnusii.
Virus-like particles (VLPs) of 40 nm diameter were isolated from the yeast-like fungus Dipodascus magnusii. These VLPs copurify with several linear double-stranded RNA molecules of different size. We have found some polymorphism in both the length and the number of these dsRNAs among six D. magnusii strains. Analysis of CsCl gradient-purified VLPs on PAGE/SDS electrophoresis showed one major protein component with an apparent molecular weight of 75 kDa.
Nosek
J
J
Department of Genetics, Faculty of Science, Comenius University, Bratislava, Czechoslovakia.
Filipp
D
D
Bederkova
K
K
Griac
P
P
D005658:Fungi / Q000235:genetics*; D008854:Microscopy, Electron; D008970:Molecular Weight; D011110:Polymorphism, Genetic; D012328:RNA Viruses / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification* / Q000648:ultrastructure; D012330:RNA, Double-Stranded / Q000235:genetics; D014764:Viral Proteins / Q000737:chemistry
D012330:RNA, Double-Stranded; D014764:Viral Proteins