Purification of recombinant hepatitis delta antigen expressed in E. coli cells.
Recombinant DNA technology enables the massive production of recombinant hepatitis delta antigen (recHDAg) retaining immunological properties and transport functions. However, purification procedures of the recombinant delta antigen have, to date, not been described in the literature. We present a purification procedure allowing one to obtain highly purified recHDAg from bacterial cells expressing the hepatitis delta antigen.
Calogero
R
R
Dipartimento di Genetica, Biologia Generale e Molecolare, Università di Napoli FedericoII, Italy.
Barbieri
U
U
Borla
M
M
Osborne
S
S
Poisson
F
F
Bonelli
F
F
D000956:Antigens, Viral / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism*; D003698:Hepatitis Delta Virus / Q000235:genetics / Q000276:immunology*; D012367:RNA, Viral / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D001616:beta-Galactosidase
D000956:Antigens, Viral; D012367:RNA, Viral; D011993:Recombinant Fusion Proteins; D001616:beta-Galactosidase