title: The induction of IL-1 by freeze-dried ethylene oxide-treated bone-patellar tendon-bone allograft wear particles: an in vitro study.
abstract: There have been recent reports of adverse clinical results with freeze-dried ethylene oxide-treated bone-patellar tendon-bone (FD-ETO-BPTB) allografts used in anterior cruciate ligament (ACL) reconstruction. Ethylene oxide and its residues were implicated as the cause of many of the failures. Wear particles generated from both freeze-dried ethylene oxide-treated and deep frozen bone-patellar tendon-bone (DF-BPTB) allografts were placed in culture with lapine synoviocytes. The resulting synovial-conditioned media were then assayed for interleukin-1 (IL-1) content. IL-1 is a potent mediator of tissue inflammation. FD-ETO-BPTB wear particles generated statistically significant levels of IL-1 when compared with both a negative control and DF-BPTB wear particles.
authors.affiliation: Ferguson Laboratory for Orthopaedic Research, University of Pittsburgh School of Medicine, Pennsylvania 15261.
authors.forename: V J
authors.initials: VJ
authors.lastname: Silvaggio
authors.forename: F H
authors.initials: FH
authors.lastname: Fu
authors.forename: H I
authors.initials: HI
authors.lastname: Georgescu
authors.forename: C H
authors.initials: CH
authors.lastname: Evans
mesh_terms: D016118:Anterior Cruciate Ligament / Q000276:immunology*; D016025:Bone Transplantation / Q000276:immunology*; D002460:Cell Line; D005027:Ethylene Oxide; D005612:Freeze Drying; D006801:Humans; D007375:Interleukin-1 / Q000096:biosynthesis*; D010329:Patella / Q000276:immunology*; D013582:Synovial Fluid / Q000166:cytology; D013710:Tendons / Q000276:immunology* / Q000637:transplantation
chemical_list: D007375:Interleukin-1; D005027:Ethylene Oxide