Generation of bidirectional deletions using DNase I.
As a result of the sporadic success we have had with established deletion-generating methods, a simpler and more reliable method was developed. This method that allows efficient and reliable recovery of deletion subclones is a simple modification of earlier DNase I protocols. We have found that the choice of linear double-stranded DNA substrates eliminates many of the problems associated with the earlier protocols.
Eberle
J R
JR
Department of Biology, Syracuse University, NY 13244.
D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D003850:Deoxyribonuclease I; D004926:Escherichia coli / Q000235:genetics; D017353:Gene Deletion; D005798:Genes, Bacterial; D005821:Genetic Techniques; D005822:Genetic Vectors; D014170:Transformation, Genetic
D004269:DNA, Bacterial; D003850:Deoxyribonuclease I