Cloning and characterization of EagI YACs from human chromosome 21.
Yeast artificial chromosomes (YACs) were made from a total EagI digest of DNA from a mouse-human chromosome 21 hybrid cell line. Approximately 3750 YACs, corresponding to 75-125 human YACs, with an average size of approximately 100 kb were recovered. Southern hybridization indicates that the chimera frequency in this library may be less than 3%. Thirty-four of the human EagI YACs were regionally assigned by a number of methods. Some YACs were regionally assigned to one of six chromosome regions by hybridization of Alu-PCR products from the YAC against Alu-PCR-amplified DNA from a panel of hybrid cell lines that contain various parts of chromosome 21. Additional YACs were regionally assigned by fluorescence in situ hybridization using either biotinylated Alu-PCR products or yeast genomic DNA from the YAC-containing strains as probes. The regionally assigned EagI YACs are located preferentially in two regions of the chromosome: near the q telomere and in the p-arm ribosomal gene region.
Gingrich
J C
JC
Human Genome Center, Lawrence Berkeley Laboratory, Berkeley, California 94720.
Lowry
S R
SR
Kuo
W L
WL
Gray
J
J
Smith
C L
CL
Cantor
C R
CR
D000818:Animals; D001483:Base Sequence; D015825:Chromosomes, Fungal; D002891:Chromosomes, Human, Pair 21; D003001:Cloning, Molecular; D004277:DNA, Single-Stranded; D015252:Deoxyribonucleases, Type II Site-Specific; D015894:Genome, Human; D015698:Genomic Library; D006801:Humans; D006822:Hybrid Cells; D051379:Mice; D008969:Molecular Sequence Data
D004277:DNA, Single-Stranded; C046751:endodeoxyribonuclease XmaIII; D015252:Deoxyribonucleases, Type II Site-Specific