Morphogenic capabilities of human immunodeficiency virus type 1 gag and gag-pol proteins in insect cells.
To determine the interaction between the gag precursor and the viral protease and to confirm the role of gag precursor in formation of human immunodeficiency virus type 1 particles, the gag and protease encoding regions of a proviral genome with mutations at the site between p17 and p24 or p24 and p15 were expressed by recombinant baculoviruses under the transcriptional control of the strong polyhedrin promoter. Western blot analyses of the expressed products of p17-p24 mutated viruses revealed that both 41- and 55-kDa proteins were synthesized. However, free p24, p17, and the other smaller cleavage products (p9, p6) could not be detected in infected insect cells. The second recombinant virus (p24-p15) synthesized not only a 55k-Da protein, but also a number of smaller products including a 40k-Da protein, p24, and p17. Examination of the insect cells infected by either of these two recombinant viruses by electron microscopy failed to detect any gag particle formation, although some irregular membrane protrusions and profound distortions of the cell surface were clearly visible in the cells infected with recombinant mutant p17-p24 virus, but not with recombinant p24-p15 mutants. To investigate the morphogenic capability of the gag-pol fusion protein, a mutant gag-pol gene containing an inactive protease as well as a modified gag-pol gene lacking the frameshifting activity were expressed in insect cells. While the inactive protease mutant was capable of forming immature particles that were secreted, the frameshifting mutant synthesized only an aberrant form of gag particles with a large radius of curvature in lieu of spherical particles. However, when this mutant was expressed in insect cells in the presence of a truncated gag protein with M(r) of 46 kDa (lacking only the p6 domain), normal immature particles containing both antigens were formed.
Hughes
B P
BP
Laboratory of Molecular Biophysics, University of Oxford, United Kingdom.
Booth
T F
TF
Belyaev
A S
AS
McIlroy
D
D
Jowett
J
J
Roy
P
P
D000595:Amino Acid Sequence; D000818:Animals; D015685:Fusion Proteins, gag-pol / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D015683:Gene Products, gag / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D015497:HIV-1 / Q000235:genetics / Q000502:physiology* / Q000648:ultrastructure; D007915:Lepidoptera / Q000166:cytology / Q000382:microbiology; D008969:Molecular Sequence Data; D009024:Morphogenesis / Q000502:physiology; D009154:Mutation; D011994:Recombinant Proteins / Q000096:biosynthesis
D015685:Fusion Proteins, gag-pol; D015683:Gene Products, gag; D011994:Recombinant Proteins