title: High-performance liquid chromatographic determination of cardenolides in Digitalis leaves after solid-phase extraction.
abstract: For HPLC analysis of cardenolide glycosides of Digitalis lanata the separation of the main compounds from other constituents is useful. An improved method for doing this, based on solid-phase extraction on a C18 modified poly(styrene-divinylbenzene) polymer, is described. The presented assay permits quantitative estimation of more than 50 cardenolides in about 2 mg of dried leaf powder of Digitalis lanata with high speed and accuracy.
authors.affiliation: Institut für Pharmazeutische Biologie, Philipps-Universität, Marburg/Lahn, Germany.
authors.forename: H
authors.initials: H
authors.lastname: Wiegrebe
authors.forename: M
authors.initials: M
authors.lastname: Wichtl
mesh_terms: D002240:Carbohydrate Sequence; D002298:Cardenolides / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004070:Digitalis / Q000737:chemistry*; D008969:Molecular Sequence Data; D010946:Plants, Medicinal; D010947:Plants, Toxic
chemical_list: D002298:Cardenolides