Purification, crystallization, and preliminary X-ray studies on the rhizome lectin from stinging nettle and its complex with NN'N"-triacetylchitotriose.
Single crystals were grown from affinity-purified stinging nettle lectin and from its complex with the specific trisaccharide NN'N"-triacetylchitotriose by vapor diffusion at room temperature. The lectin crystallizes in space group P2(1)2(1)2(1) with unit cell dimensions a = 54.3 (1) A, b = 62.2 (1) A, and c = 92.4 (2) A, and diffracts to 3.0 A resolution. The asymmetric unit contains three lectin monomers. The crystals of the lectin-trisaccharide complex have space group P2(1)2(1)2(1) with cell constants a = 37.69 (4) A, b = 48.97 (6) A, and c = 57.32 (4) A. These crystals diffract to at least 2.0 A resolution and the asymmetric unit contains one lectin monomer. A three-dimensional X-ray structure determination is on its way.
Loris
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Vrije Universiteit Brussel, Instituut voor Moleculaire Biologie, Sint-Genesius-Rode, Belgium.
Thi
M H
MH
Lisgarten
J
J
Wyns
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L
D001665:Binding Sites; D003460:Crystallization; D037102:Lectins / Q000737:chemistry* / Q000302:isolation & purification; D015394:Molecular Structure; D037121:Plant Lectins; D010944:Plants / Q000737:chemistry; D014312:Trisaccharides / Q000737:chemistry; D014961:X-Ray Diffraction
D037102:Lectins; D037121:Plant Lectins; D014312:Trisaccharides; C019117:N,N',N''-triacetylchitotriose