title: The effect of basic fibroblast growth factor on the growth and function of human thyrocytes.
abstract: Basic fibroblast growth factor (bFGF) was quantitated in human primary thyrocyte cultures and thyroid cell lines produced by transfection with pSV3neo. Immunoreactive-bFGF (ir-bFGF) bound to heparin-Sepharose affinity columns eluted with 1.8-2.0 mol NaCl/l and had a molecular weight of approximately 17,000. Recombinant human bFGF in the presence of 5% serum increased the growth of transfected human thyrocytes but not the growth of primary human thyrocytes. Preincubation of cells with up to 100 micrograms bFGF/l potentiated TSH-stimulated cAMP release from the transfected cells but inhibited release from primary human thyroid cultures. bFGF may be an important modulator of thyroid cell function and growth.
authors.affiliation: Department of Cellular and Molecular Sciences, St George's Hospital Medical School, London, U.K.
authors.forename: A H
authors.initials: AH
authors.lastname: Taylor
authors.forename: L J
authors.initials: LJ
authors.lastname: Millatt
authors.forename: G S
authors.initials: GS
authors.lastname: Whitley
authors.forename: A P
authors.initials: AP
authors.lastname: Johnstone
authors.forename: S S
authors.initials: SS
authors.lastname: Nussey
mesh_terms: D002478:Cells, Cultured; D002846:Chromatography, Affinity; D016222:Fibroblast Growth Factor 2 / Q000032:analysis / Q000378:metabolism*; D006801:Humans; D011863:Radioimmunoassay; D013961:Thyroid Gland / Q000737:chemistry / Q000166:cytology / Q000254:growth & development / Q000502:physiology*
chemical_list: D016222:Fibroblast Growth Factor 2