A gene encoding a small, acid-soluble spore protein from alkaliphilic Bacillus firmus OF4.
An 1100-bp DNA fragment cloned from alkaliphilic Bacillus firmus OF4 contained an open reading frame deduced to encode a 54-amino-acid, glutamine-rich protein with 35.6% identity to Bacillus subtilis small, acid-soluble spore protein-gamma (SASP-gamma) in a 45-aa overlap. This ORF, designated sspA, lacks the lengthy sequence repeat characteristic of previously cloned SASP-gamma-encoding genes. Southern analysis under conditions of moderate stringency revealed six bands, suggesting the presence of several related genes in the alkaliphile.
Quirk
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Department of Biochemistry, Mount Sinai School of Medicine, CUNY, NY 10029.
D000595:Amino Acid Sequence; D001407:Bacillus / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D012808:Sigma Factor; D013171:Spores, Bacterial / Q000235:genetics; D014157:Transcription Factors
D001426:Bacterial Proteins; D012808:Sigma Factor; D014157:Transcription Factors; C092468:spoIIR protein, Bacillus subtilis; C030069:spore-specific proteins, Bacillus