Interleukin-1-dependent mitogenic responses induced by protoscoleces of Echinococcus multilocularis in murine lymphocytes.
Mitogenic effects of protoscoleces (PSCs) of Echinococcus multilocularis on murine lymphocytes were studied. Spleen cells from normal BALB/c mice showed significant proliferative responses when cocultured with PSCs. Proliferative responses were observed in both the T and B cell populations. The PSCs also stimulated cells of the macrophage/monocyte lineage to secrete interleukin-1 (IL-1). Depletion of plastic- and Sephadex G-10-adherent cells from the spleen cell population significantly reduced the proliferative responses to PSCs and the low responsiveness was restored by addition of plastic-adherent cells to these cultures. Furthermore, addition of anti-IL-1 serum to the spleen cell cultures stimulated with PSCs completely suppressed the proliferative responses. These findings demonstrate that the mitogenic effect of PSC on lymphocytes depends on IL-1 secreted by cells of macrophage/monocyte lineage.
Kizaki
T
T
Hokkaido Institute of Public Health, Japan.
Ishige
M
M
Bingyan
W
W
Kumagai
M
M
Day
N K
NK
Good
R A
RA
Onoé
K
K
D000818:Animals; D001402:B-Lymphocytes / Q000469:parasitology; D002448:Cell Adhesion; D004443:Echinococcosis / Q000469:parasitology*; D004446:Echinococcus / Q000187:drug effects / Q000276:immunology*; D005260:Female; D007106:Immune Sera / Q000494:pharmacology; D007375:Interleukin-1 / Q000276:immunology / Q000494:pharmacology*; D008213:Lymphocyte Activation / Q000276:immunology; D016131:Lymphocyte Subsets / Q000469:parasitology; D008214:Lymphocytes / Q000469:parasitology*; D008264:Macrophages / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008934:Mitogens / Q000276:immunology*; D009000:Monocytes / Q000378:metabolism; D013154:Spleen / Q000166:cytology / Q000378:metabolism / Q000469:parasitology; D013601:T-Lymphocytes / Q000469:parasitology
D007106:Immune Sera; D007375:Interleukin-1; D008934:Mitogens