title: Cytogenetic changes in primary, immortalized and malignant mammalian cells.
abstract: Some chromosomes in transformed rat cells and somatic cell hybrids fail to display the presence of kinetochore proteins as detected by antikinetochore antibodies. Such chromosomes (K- chromosomes) may constitute a novel mechanism for the genesis of aneuploidy. We have analyzed primary, immortalized and malignant mammalian cells for the presence of kinetochore proteins and micronuclei. Our results suggest a correlation of the K- chromosome and micronucleus frequency with the variability in chromosome number. Upon in situ hybridization with the minor satellite and alpha satellite sequences some K- chromosomes showed a signal. This indicates that the observed lack of kinetochores is not necessarily due to a lack of centromeric DNA. We conclude that dislocated K- chromosomes may become incorporated into micronuclei which are prone to loss. Such events would be associated with the generation of aneuploidy.
authors.affiliation: Institut für Toxikologie, Universität Würzburg, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Kirchner
authors.forename: H
authors.initials: H
authors.lastname: Stopper
authors.forename: T
authors.initials: T
authors.lastname: Papp
authors.forename: I
authors.initials: I
authors.lastname: Eckert
authors.forename: H J
authors.initials: HJ
authors.lastname: Yoo
authors.forename: B K
authors.initials: BK
authors.lastname: Vig
authors.forename: D
authors.initials: D
authors.lastname: Schiffmann
mesh_terms: D016475:3T3 Cells; D000818:Animals; D002460:Cell Line; D002461:Cell Line, Transformed; D002503:Centromere / Q000502:physiology*; D002869:Chromosome Aberrations; D006224:Cricetinae; D006367:HeLa Cells; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007739:L Cells; D008647:Mesocricetus; D008677:Metaphase / Q000235:genetics; D051379:Mice; D048629:Micronuclei, Chromosome-Defective; D014407:Tumor Cells, Cultured