Procollagen binding to sphingomyelin.
The interactions of [3H]procollagen I with various phospholipids were studied by density gradient centrifugation. At physiological conditions of pH, ionic strength, and temperature, there was no evidence for procollagen binding to phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol, or phosphatidylserine liposomes. In contrast, procollagen I bound strongly to sphingomyelin liposomes in a reversible and saturable manner, with an apparent dissociation constant (Kd) of 2.6 nM. Binding occurred over a range of temperatures (4-37 degrees C) and was relatively unaffected by salt concentrations up to 1.2 M NaCl. Binding was observed in phosphate buffers, but not in the presence of high concentrations of Tris or Hepes. Bovine serum albumin had no effect on procollagen binding to sphingomyelin, and neither did unlabeled type I collagen, with or without the nonhelical telopeptides. Procollagen II and denatured procollagen I also bound to sphingomyelin. Procollagen binding to sphingomyelin at 35 degrees C was considerably reduced when small amounts of phosphatidylcholine were present, although binding was partially restored when the temperature was reduced below the corresponding phase transition temperature. Purified unlabeled procollagen COOH-terminal propeptides successfully competed for binding, and 125I-labeled COOH-terminal propeptides bound to sphingomyelin in the absence of procollagen. Weaker binding to sphingomyelin, mediated by the collagen triple helical region, was also observed; but this was dominated by the sphingomyelin-COOH-terminal propeptide interaction. The data suggest a novel mechanism for matrix vesicle-mediated biomineralization.
Choglay
A A
AA
Department of Biochemistry, University of Edinburgh, United Kingdom.
Purdom
I F
IF
Hulmes
D J
DJ
D000818:Animals; D002021:Buffers; D002417:Cattle; D002498:Centrifugation; D002642:Chick Embryo; D003094:Collagen / Q000378:metabolism; D004134:Dimyristoylphosphatidylcholine; D050356:Lipid Metabolism; D008081:Liposomes / Q000737:chemistry / Q000378:metabolism; D011347:Procollagen / Q000378:metabolism*; D011485:Protein Binding; D013109:Sphingomyelins / Q000378:metabolism*; D013696:Temperature
D002021:Buffers; D008081:Liposomes; D011347:Procollagen; D013109:Sphingomyelins; D003094:Collagen; D004134:Dimyristoylphosphatidylcholine