title: Human and bovine articular cartilage loading measured with a new resistive sensor: effects of protease degradation and osteoarthritis.
abstract: This report describes the use of a new micro-thin resistive-based sensor to measure dynamic vertical loading of normal and osteoarthritic (OA) human knee cartilage repetitively under conditions of simulated knee joint loading. Articular cartilage discs were cultured in 3 DMEM +/- IL-1, lipopolysacharide (LPS) and trypsin over 7 days. Loading force, force distribution and peak pressure were evaluated. The sensor detected early stiffness and dynamic loading changes due to IL-1, LPS and trypsin treatment, and differences between normal and early OA human cartilages showing decreased cartilage stiffness due to enzyme treatment and osteoarthritis.
authors.affiliation: Orthopaedic Research Laboratories, Massachusetts General Hospital, Boston.
authors.forename: B J
authors.initials: BJ
authors.lastname: Awbrey
authors.forename: C A
authors.initials: CA
authors.lastname: Richlen
authors.forename: M
authors.initials: M
authors.lastname: Golpovar
mesh_terms: D000818:Animals; D015374:Biosensing Techniques; D002358:Cartilage, Articular / Q000378:metabolism / Q000502:physiology* / Q000503:physiopathology; D002417:Cattle; D004553:Electric Conductivity; D006801:Humans; D066298:In Vitro Techniques; D007375:Interleukin-1 / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D010003:Osteoarthritis / Q000503:physiopathology*; D011312:Pressure; D014357:Trypsin / Q000494:pharmacology
chemical_list: D007375:Interleukin-1; D008070:Lipopolysaccharides; D014357:Trypsin