Low oxygen stimulates proliferation of fibroblasts seeded as single cells.
In standard tissue culture conditions (20% oxygen), single human dermal fibroblasts (one cell per well) do not proliferate. We now report that low oxygen tension is a potent stimulus for the proliferation and expansion of human adult and neonatal dermal fibroblasts seeded as single cells. This preferential single-cell proliferation in low oxygen is shown to be also a feature of human lung and dermal rodent fibroblasts, but not of human fibrosarcoma and immortalized 3T3 cells, which proliferate without difficulty in standard oxygen conditions. It is suggested that single-cell proliferation and its dramatic stimulation in low oxygen may represent a fundamental biologic process with an opportunity to better understand mammalian cell growth regulation.
Falanga
V
V
Department of Dermatology and Cutaneous Surgery, University of Miami School of Medicine, Florida 33101.
Kirsner
R S
RS
D016475:3T3 Cells; D000818:Animals; D002452:Cell Count; D002455:Cell Division; D015687:Cell Hypoxia; D002460:Cell Line; D002478:Cells, Cultured; D003470:Culture Media; D005347:Fibroblasts / Q000166:cytology*; D006801:Humans; D051379:Mice; D051381:Rats; D014407:Tumor Cells, Cultured
D003470:Culture Media