Expression of an ABA-responsive osmotin-like gene during the induction of freezing tolerance in Solanum commersonii.
We have isolated a cDNA (pA13) of an ABA-responsive gene from suspension cultures of Solanum cultures of Solanum commersonii. The deduced amino acid sequence of pA13 cDNA revealed 89 and 91% identity with tobacco osmotin and tomato NP24 protein, respectively. The accumulation of the transcript corresponding to pA13 cDNA was regulated by ABA, cold temperature, and low water potential treatments. Cold-induced accumulation of the pA13 transcript was partially suppressed by fluridone, an ABA synthesis inhibitor, and the suppression was restored by exogenous ABA application. The transcript corresponding to pA13 also accumulated in an organ-specific manner in response to ABA or cold treatment.
Zhu
B
B
Department of Horticultural Sciences, University of Minnesota, St. Paul 55108.
Chen
T H
TH
Li
P H
PH
D000040:Abscisic Acid / Q000494:pharmacology*; D000595:Amino Acid Sequence; D001483:Base Sequence; D002478:Cells, Cultured; D003001:Cloning, Molecular; D005615:Freezing; D005786:Gene Expression Regulation; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D016415:Sequence Alignment; D014882:Water-Electrolyte Balance
D010940:Plant Proteins; D012333:RNA, Messenger; C073139:osmotin protein, Nicotiana tabacum; D000040:Abscisic Acid