UmuC product contributes to the inhibition of dimer excision produced by thymine-less-amino acid-less pretreatment in UV-irradiated Escherichia coli.
In UV-irradiated Escherichia coli, predamaged by thymine-amino acid starvation or a UV predose, a large amount of dimers may remain unexcised and may be tolerated by an error-free mechanism, which requires the function of uvr, recA and lexA genes. A possible role of the umuC gene in both the inhibition of dimer excision and the toleration of unexcised dimers is investigated. Data suggest that the UmuC gene product is not absolutely necessary for the inhibition of dimer excision in UV-irradiated thymine-less-amino acid-less pretreated cells, but that it contributes to it. However, the UmuC product does not seem to be involved in the toleration of unexcised dimers which is dependent on uvr, recA and lexA.
Masek
F
F
Slovak Academy of Sciences, Department of Molecular Genetics, Bratislava.
Sedliaková
M
M
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D004259:DNA-Directed DNA Polymerase; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects*; D029968:Escherichia coli Proteins; D005798:Genes, Bacterial; D007700:Kinetics; D011740:Pyrimidine Dimers / Q000378:metabolism*; D011938:Rec A Recombinases / Q000235:genetics / Q000378:metabolism; D012697:Serine Endopeptidases; D013941:Thymine / Q000378:metabolism*; D014466:Ultraviolet Rays; D000099160:LexA Repressor Protein
D001426:Bacterial Proteins; D004259:DNA-Directed DNA Polymerase; D029968:Escherichia coli Proteins; D011740:Pyrimidine Dimers; D011938:Rec A Recombinases; D012697:Serine Endopeptidases; D013941:Thymine; D000099160:LexA Repressor Protein; C078202:UmuC protein, E coli