Neurotrophin-3 upregulates NGF receptors in a central nervous system glial cell line.
The low-affinity nerve growth factor (NGF) receptor (LNGFR) binds the neurotrophins NGF, brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3) with similar affinities. Here we report on the ability of NT-3 to regulate the expression of the LNGFR in C6 glioma cells. LNGFR-like immunoreactivity (LNGFR-IR) was examined in C6 cells treated for 16 h with NT-3 and exposed to the antibody 192-IgG followed by immunoglobulins conjugated with colloidal gold by means of ultrastructural morphometric analysis. Untreated C6 cells exhibited some positive LNGFR-IR, while C6 cells treated with NT-3 displayed significantly increased (2.3 fold) LNGFR-IR. The increase in LNGFR protein was accompanied by a greater quantity of LNGFR mRNA in NT-3-treated cells. Thus, LNGFR can be upregulated by the structurally related neurotrophin NT-3.
Spoerri
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PE
Fidia Research Laboratories, Fidia S.p.A., Abano Terme, Italy.
Romanello
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S
Petrelli
L
L
Negro
A
A
Guidolin
D
D
Skaper
S D
SD
D000818:Animals; D015152:Blotting, Northern; D001923:Brain Chemistry; D002460:Cell Line; D005910:Glioma / Q000378:metabolism; D016253:Microscopy, Immunoelectron; D009414:Nerve Growth Factors / Q000494:pharmacology*; D009423:Nervous System Neoplasms / Q000378:metabolism; D009457:Neuroglia / Q000187:drug effects / Q000378:metabolism*; D020933:Neurotrophin 3; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017475:Receptors, Nerve Growth Factor / Q000187:drug effects*; D011994:Recombinant Proteins / Q000494:pharmacology; D014407:Tumor Cells, Cultured; D015854:Up-Regulation / Q000187:drug effects*
D009414:Nerve Growth Factors; D020933:Neurotrophin 3; D012333:RNA, Messenger; D017475:Receptors, Nerve Growth Factor; D011994:Recombinant Proteins