Simultaneous determination of trimipramine and desmethyl- and hydroxytrimipramine in plasma and red blood cells by capillary gas chromatography with nitrogen-selective detection.
A simple procedure is described that permits the simultaneous determination of trimipramine and its two major metabolites, desmethyl- and hydroxytrimipramine, in human plasma or red blood cells (RBCs) at therapeutic concentrations. The extracted biological fluids are injected into a capillary gas chromatograph with an OV-1 fused-silica column coupled to a nitrogen-phosphorus-selective detector. The limit of determination for trimipramine is 3 ng/ml and for that desmethyl- and hydroxytrimipramine is 4 ng/ml. The method permits the RBC/plasma ratios to be determined and to be correlated with the clinical response.
Jourdil
N
N
Laboratoire de Pharmacologie, Centre Hospitalier Universitaire de Grenoble, France.
Pinteur
B
B
Vincent
F
F
Marka
C
C
Bessard
G
G
D002849:Chromatography, Gas / Q000379:methods*; D004912:Erythrocytes / Q000737:chemistry*; D006801:Humans; D009584:Nitrogen / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014299:Trimipramine / Q000031:analogs & derivatives / Q000097:blood*
C070661:2-hydroxytrimipramine; D014299:Trimipramine; C040606:desmethyltrimipramine; D009584:Nitrogen