title: Crystallization and preliminary X-ray crystallographic analysis of arylesterase from Pseudomonas fluorescens.
abstract: Large crystals of arylesterase from Pseudomonas fluorescens have been grown at room temperature using ammonium sulfate as a precipitant. They grow to dimensions of 0.7 x 0.7 x 0.6 mm3 within a month. The crystals belong to the trigonal space group P3(1) (or P3(2)), with unit cell dimensions of a = 147.12 A and c = 131.08 A. The asymmetric unit seems to contain six molecules of dimeric arylesterase, with corresponding crystal volume per protein mass (VM) of 2.53 A3/Da and solvent fraction of 51.5% by volume. The crystals diffract to at least 2.2 A Bragg spacing when exposed to X-rays from a rotating-anode source. X-ray data have been collected to 2.9 A Bragg spacing from native crystals.
authors.affiliation: Department of Chemistry, College of Natural Sciences, Seoul National University, Korea.
authors.forename: K K
authors.initials: KK
authors.lastname: Kim
authors.forename: K Y
authors.initials: KY
authors.lastname: Hwang
authors.forename: K D
authors.initials: KD
authors.lastname: Choi
authors.forename: J H
authors.initials: JH
authors.lastname: Kang
authors.forename: O J
authors.initials: OJ
authors.lastname: Yoo
authors.forename: S W
authors.initials: SW
authors.lastname: Suh
mesh_terms: D002265:Carboxylic Ester Hydrolases / Q000737:chemistry*; D003460:Crystallization; D011551:Pseudomonas fluorescens / Q000201:enzymology*; D014961:X-Ray Diffraction
chemical_list: D002265:Carboxylic Ester Hydrolases; C019734:arylesterase