Detection of antigens in the urine of patients with acute Plasmodium vivax malaria.
Plasmodium antigens were detected by dot-blot assay in the urine of 50 patients infected with Plasmodium vivax. Antigens also were detected in 12/15 patients who no longer had detectable parasitemia, 3 weeks after chemotherapy. Antigenuria was negative 6 weeks after treatment. By Western blotting, four predominant protein antigens were identified in the urine of patients infected with P. vivax: 200, 180, 150, and 110 kDa. The dot-blot technique may prove to be a rapid and inexpensive method for diagnosing malaria in field studies and for clinical evaluation during chemotherapy.
Militão
D N
DN
Department of Parasitology, University of São Paulo, Brazil.
Camargo
L M
LM
Katzin
A M
AM
D000293:Adolescent; D000328:Adult; D000818:Animals; D000953:Antigens, Protozoan / Q000652:urine*; D015153:Blotting, Western; D005260:Female; D006801:Humans; D015151:Immunoblotting; D016780:Malaria, Vivax / Q000175:diagnosis* / Q000652:urine; D008297:Male; D008875:Middle Aged; D010966:Plasmodium vivax / Q000276:immunology*
D000953:Antigens, Protozoan