Propofol decreases random and chemotactic stimulated locomotion of human neutrophils in vitro.
We have studied the influence of clinical concentrations of propofol (2,6-diisopropyl phenol), emulsified propofol (Diprivan) and the emulsifier of propofol (Intralipid 10%) on random and chemotactic locomotion of human polymorphonuclear leucocytes in an agarose assay. Random locomotion was decreased (P < 0.001) to a similar extent by the three drugs. Concentrations of propofol 2.5 micrograms ml-1 and greater, and of Diprivan 3.33 micrograms ml-1 and greater, also reduced chemotaxis (P < 0.05) against both zymosan-activated human serum (C5a) and N-formyl-methionyl-leucylphenylalanine (FMLP), used as chemoattractants. Intralipid reduced chemotaxis towards C5a but not towards FMLP. We conclude that propofol in clinically relevant concentrations may adversely affect leucocyte locomotion in vitro.
Jensen
A G
AG
Department of Anaesthesiology, Faculty of Health Sciences, Linköping University Hospital, Sweden.
Dahlgren
C
C
Eintrei
C
C
D000328:Adult; D002465:Cell Movement / Q000187:drug effects; D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D015936:Complement C5a / Q000494:pharmacology; D003864:Depression, Chemical; D004305:Dose-Response Relationship, Drug; D005079:Excipients; D006801:Humans; D066298:In Vitro Techniques; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects; D015742:Propofol / Q000494:pharmacology*
D005079:Excipients; D009240:N-Formylmethionine Leucyl-Phenylalanine; D015936:Complement C5a; D015742:Propofol