Identification of proteins encoded by avian reoviruses and evidence for post-translational modification.
Twelve avian reovirus strain 176 proteins, 10 structural (lambda 1, lambda 2, lambda 3, mu 1, mu 2/mu 2C, sigma 1, sigma 2, sigma 3, and sigma 4), and 2 nonstructural (mu NS and sigma NS) were identified and characterized by electrophoretic analysis of purified virions and infected cell lysates. Three of the identified proteins (mu 1, mu NS, and sigma 4) have not been previously described. In pulse-chase experiments, 10 of the proteins (lambda 1, lambda 2, lambda 3, mu 1, mu 2, mu NS, sigma 1, sigma 2, sigma NS, sigma 3) were shown to be primary translation products, whereas mu 2C was shown to be a post-translational cleavage product of mu 2.
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Department of Veterinary Pathobiology, College of Veterinary Medicine, Texas A&M University, College Station 77843-4467.
Ramig
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Kemp
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D000818:Animals; D002642:Chick Embryo; D011499:Protein Processing, Post-Translational / Q000502:physiology*; D012087:Reoviridae / Q000737:chemistry*; D017361:Viral Nonstructural Proteins / Q000032:analysis; D014764:Viral Proteins / Q000032:analysis*; D015678:Viral Structural Proteins / Q000032:analysis
D017361:Viral Nonstructural Proteins; D014764:Viral Proteins; D015678:Viral Structural Proteins