Neurite outgrowth from N18TG2 neuroblastoma induced by H-7, a protein kinase inhibitor, in the presence of colchicine.
Our previous studies have demonstrated that the protein kinase inhibitor H-7 promotes neurite outgrowth from mouse neuroblastoma N18TG2 cells as well as from primary cerebellar cells, and also that the neurites induced by H-7 were more tolerant of colchicine (COL) than those induced by dibutyryl cAMP (dB-cAMP). In the present study, we tested the effects of H-7 and dB-cAMP on neurite growth from N18TG2 cells in the presence of COL. We found that only H-7 promoted neurite formation in the presence of COL. The percentage of cells with neurites induced by H-7 in the presence of COL (H-7 + COL) was similar to that induced by H-7 alone. The neurites induced by H-7 + COL grew straight. They were very thin (less than 1 micron in diameter) and had round varicosities, as did the neurites induced by H-7 alone. By transmission electron microscopy, the neurites induced by H-7 + COL were found to contain longitudinally arranged intermediate filaments (IF). Microtubules (MT) were not observed within the neurites. We also examined the effect of cytochalasin B (CB) on the neurites induced by H-7 + COL and by H-7 alone. The neurites induced by H-7 + COL were tolerant to CB, but those induced by H-7 were resorbed completely within 24 h after CB was applied. Neurites tolerant to CB contained longitudinally IF. Simultaneous application of CB with H-7 + COL or with H-7 alone did not induce neurite formation.(ABSTRACT TRUNCATED AT 250 WORDS)
Ono
K
K
Third Department of Anatomy, School of Medicine, Okayama University, Japan.
Tokunaga
A
A
Tsuda
M
M
D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D000818:Animals; D003078:Colchicine / Q000494:pharmacology*; D003571:Cytochalasin B / Q000494:pharmacology; D007546:Isoquinolines / Q000494:pharmacology*; D016501:Neurites / Q000187:drug effects* / Q000502:physiology; D009447:Neuroblastoma / Q000473:pathology / Q000503:physiopathology* / Q000648:ultrastructure; D010879:Piperazines / Q000494:pharmacology*; D047428:Protein Kinase Inhibitors; D014407:Tumor Cells, Cultured
D007546:Isoquinolines; D010879:Piperazines; D047428:Protein Kinase Inhibitors; D003571:Cytochalasin B; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D003078:Colchicine