Treatment responses to surfactants containing natural surfactant proteins in preterm rabbits.
The in vivo function of surfactants reconstituted using natural surfactant lipid and protein constituents was evaluated in 27-day-gestation preterm rabbits. The animals were treated with protein-free surfactant lipids (LH-20), LH-20 + 5% SP-A, LH-20 + 1% SP-B, LH-20 + 1% SP-C, LH-20 + 5% SP-A + 1% SP-B + 1% SP-C (SP-ABC), natural sheep surfactant, or 4 ml/kg 0.45% NaCl (control) and then ventilated with tidal volumes of 8 ml/kg and 3 cm H2O positive end-expiratory pressure (PEEP). Ventilatory pressures (peak pressures minus PEEP) and dynamic compliances of the LH-20 + SP-C rabbits were greater (p < 0.01) than those of control, LH-20, and LH-20 + SP-A groups but lower (p < 0.05) than in the LH-20 + SP-B, LH-20 + SP-ABC, and sheep surfactant groups. Recoveries of intravascular labeled albumin in the lungs were comparable in the LH-20 + SP-B, LH-20 + SP-C, LH-20 + SP-ABC, and sheep surfactant groups and less (p < 0.01) than in LH-20 + SP-A rabbits, which had lower (p < 0.05) recoveries than did the control and LH-20 groups. The postventilation pressure-volume curves for LH-20 + SP-B and LH-20 + SP-ABC rabbits had significantly lower opening pressures, larger maximal lung volumes, and larger retained volumes on deflation relative to the LH-20 + SP-C, LH-20, and control groups.(ABSTRACT TRUNCATED AT 250 WORDS)
Rider
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Harbor-UCLA Medical Center, Department of Pediatrics, Torrance 90502.
Ikegami
M
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Whitsett
J A
JA
Hull
W
W
Absolom
D
D
Jobe
A H
AH
D000818:Animals; D002585:Cesarean Section; D005260:Female; D005333:Fetus / Q000000981:diagnostic imaging / Q000187:drug effects* / Q000502:physiology; D005865:Gestational Age; D008168:Lung / Q000000981:diagnostic imaging / Q000187:drug effects / Q000502:physiology; D008170:Lung Compliance / Q000187:drug effects; D008176:Lung Volume Measurements; D011175:Positive-Pressure Respiration / Q000379:methods; D011247:Pregnancy; D011663:Pulmonary Surfactants / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D011817:Rabbits; D011877:Radionuclide Imaging; D012711:Serum Albumin, Radio-Iodinated; D012756:Sheep
D011663:Pulmonary Surfactants; D012711:Serum Albumin, Radio-Iodinated