Protein and peptide mobility in capillary zone electrophoresis. A comparison of existing models and further analysis.
Capillary zone electrophoresis of peptide fragments from the tryptic digest of human recombinant insulin-like growth factor I (rhIGF-I) has been carried out and the observed mobilities used to compare the relative applicability of existing mobility models. In addition, the physical forces affecting electromigration have been systematically analyzed in order to more accurately describe the physical chemistry involved. Such an approach should further improve the ability to predict electrophoretic mobility in capillary zone electrophoresis.
Hilser
V J
VJ
Physical and Analytical Chemistry Department, Ciba-Geigy Corporation, Suffern, NY 10901.
Worosila
G D
GD
Rudnick
S E
SE
D000595:Amino Acid Sequence; D004586:Electrophoresis / Q000379:methods*; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000737:chemistry; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D011994:Recombinant Proteins / Q000737:chemistry
D010455:Peptides; D011506:Proteins; D011994:Recombinant Proteins; D007334:Insulin-Like Growth Factor I