title: The pRSET family of T7 promoter expression vectors for Escherichia coli.
abstract: A family of eight T7 promoter-based expression plasmids is presented. These are high-copy-number vectors featuring translational start and stop elements and a multiple cloning site (polylinker) with eleven unique restriction sites in all six reading frames. Depending on the cloning strategy used, recombinant proteins may contain either short vector-encoded fusion fragments or no fusion fragments at all. Following promoter induction, proteins are usually produced at a high level.
authors.affiliation: Salk Institute, San Diego, CA 92186-5800.
authors.forename: R
authors.initials: R
authors.lastname: Schoepfer
mesh_terms: D000595:Amino Acid Sequence; D017123:Bacteriophage T7 / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods*; D004926:Escherichia coli / Q000235:genetics*; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D010957:Plasmids; D011401:Promoter Regions, Genetic; D016364:Reading Frames; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis*; D015183:Restriction Mapping; D013728:Terminator Regions, Genetic
chemical_list: D009838:Oligodeoxyribonucleotides; D011993:Recombinant Fusion Proteins