title: Isolation of feline eosinophils via peritoneal lavage.
abstract: Fourteen cats were inoculated orally with 1 of 2 infective doses of Toxocara canis to induce eosinophilia. Cats were subsequently challenge exposed twice via intraperitoneal injection with 1 of 2 T canis antigen preparations. Peritoneal lavage was performed 2 days after antigenic challenge exposure, and eosinophils in the peritoneal lavage fluid were quantified. None of the cats developed clinical signs of disease after infection. All cats developed peripheral eosinophilia after infection. Significant (P < 0.05) difference in mean eosinophil count from the lavage fluid was observed between lavage 1 (prechallenge exposure) and lavages 2 and 3 (postchallenge exposure) in both groups of cats. Significant difference in eosinophil count was not found between cats given different doses of eggs. After initial challenge exposure, significantly (P < 0.05) more eosinophils were obtained from cats given antigen preparation 2 (prep-2) than from those given antigen prep-1. This difference was no longer observed after the second challenge exposure with higher doses of either antigen prep-1 or prep-2. In cats given antigen prep-2, significant difference was not found between lavages 2 and 3. However, in cats given antigen prep-1, eosinophil count was significantly (P = 0.005) greater in fluid obtained from lavage 3, compared with eosinophil count from lavage 2. Mean +/- SEM percentage of eosinophils in the fluid from lavage 3 in all cats was 70.8 +/- 2.2%. Other cell types included macrophages, neutrophils, lymphocytes, and mast cells. Gross postmortem findings were mild. One- to 3-mm nodular white foci of inflammation were observed on the serosal surfaces of the liver, spleen, kidneys, and omentum.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Medical Sciences, University of Wisconsin, School of Veterinary Medicine, Madison 53706.
authors.forename: K A
authors.initials: KA
authors.lastname: Moriello
authors.forename: K M
authors.initials: KM
authors.lastname: Young
authors.forename: A J
authors.initials: AJ
authors.lastname: Cooley
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology; D002371:Cat Diseases / Q000097:blood / Q000276:immunology*; D002415:Cats; D002469:Cell Separation / Q000379:methods / Q000662:veterinary*; D004802:Eosinophilia / Q000097:blood / Q000276:immunology / Q000662:veterinary*; D004804:Eosinophils; D005260:Female; D007958:Leukocyte Count / Q000662:veterinary; D008297:Male; D010533:Peritoneal Lavage / Q000662:veterinary*; D017166:Toxocara canis / Q000276:immunology; D014120:Toxocariasis / Q000097:blood / Q000276:immunology
chemical_list: D000947:Antigens, Helminth