Molecular analysis of double isotype expression in IgA switching.
In this and previous studies we have shown that during the course of B cell isotype differentiation occurring in the CH12.LX B cell line, small numbers of cells appear that bear both membrane (m)IgM and membrane IgA. We have cloned relatively stable populations of such dual-bearing cells and have performed studies to determine their molecular status. In the study of one such subclone (CH12.LX.7.10), we show by primer extension that both mu and alpha mRNA contain identical VHDJH gene segments. In complementary studies of another such subclone (CH12.LX.7.13), we show using DNA restriction fragment analysis, that the C mu gene segment, but not the C alpha gene segment, is associated with the JH gene segment. Additionally, Northern blot analysis of productive message of this clone shows that both mu and alpha mRNA cohybridize to the same VH region-specific probe. Taken together, these data suggest that CH12.LX mIgM/mIgA dual-bearing cells occur in the absence of C mu gene segment deletion and that the mu and alpha mRNA transcripts are derived using an alternative mechanism such as transplicing or alternative splicing of a long RNA transcript. On the assumption that these clones are representative of mIgM/mIgA dual-bearing cells appearing during CH12.LX IgM to IgA isotype differentiation, these data support the idea that B cells simultaneously produce mu and alpha mRNA transcripts during one stage of IgA isotype switching.
Kunimoto
D Y
DY
Department of Medical Microbiology and Infectious Diseases, University of Alberta, Edmonton, Canada.
Sneller
M C
MC
Claflin
L
L
Mushinski
J F
JF
Strober
W
W
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D001483:Base Sequence; D015139:Blotting, Southern; D002478:Cells, Cultured; D002999:Clone Cells; D005434:Flow Cytometry; D015870:Gene Expression; D005786:Gene Expression Regulation; D015326:Gene Rearrangement, B-Lymphocyte, Heavy Chain; D007132:Immunoglobulin Isotypes / Q000235:genetics*; D066298:In Vitro Techniques; D051379:Mice; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics
D007132:Immunoglobulin Isotypes; D012333:RNA, Messenger