title: Development of a sperm zona pellucida binding assay for bull semen.
abstract: Assays based on sperm zona pellucida binding have been developed as diagnostic tests to predict the fertilisation potential of human spermatozoa. The aim of this study was to establish a similar assay for bull sperm. The results showed differences between established fertile bulls in the relative numbers of sperm cells bound to the zona pellucida of a batch of oocytes. These differences suggest that there may be a relationship between the sperm zona pellucida binding capacity and the fertility of bulls.
authors.affiliation: Department of Herd Health and Reproduction, Faculty of Veterinary Medicine, University of Utrecht, The Netherlands.
authors.forename: A R
authors.initials: AR
authors.lastname: Fazeli
authors.forename: W
authors.initials: W
authors.lastname: Steenweg
authors.forename: M M
authors.initials: MM
authors.lastname: Bevers
authors.forename: F A
authors.initials: FA
authors.lastname: de Loos
authors.forename: J
authors.initials: J
authors.lastname: van den Broek
authors.forename: B
authors.initials: B
authors.lastname: Colenbrander
mesh_terms: D000818:Animals; D002417:Cattle / Q000502:physiology*; D005260:Female; D005298:Fertility / Q000502:physiology*; D066298:In Vitro Techniques; D008297:Male; D009865:Oocytes / Q000502:physiology; D012661:Semen / Q000166:cytology; D013084:Sperm-Ovum Interactions / Q000502:physiology*; D015044:Zona Pellucida / Q000502:physiology*