title: Sensitive high-performance liquid chromatographic assay for the determination of chlorhexidine in saliva.
abstract: A high-performance liquid chromatographic assay was developed to determine salivary chlorhexidine concentration. Saliva sample (200 microliters) was extracted into methylene chloride. Chromatographic separation was achieved with a C18 column using a mobile phase of acetonitrile-0.05 M sodium acetate and 0.005 M heptanesulfonic acid (40:60, v/v). The standard curve was linear from 0.051 to 20.48 micrograms/ml (r2 > or = 0.997). Intra-day and inter-day coefficients of variation were < 5.5 and < or = 9%, respectively. The assay is rapid, sensitive, simple, and successfully used for quantitating salivary chlorhexidine content released from a chlorhexidine-impregnated resin worn by patients.
authors.affiliation: Department of Pharmacology, University of Texas Health Science Center, San Antonio 78284.
authors.forename: Y W
authors.initials: YW
authors.lastname: Lam
authors.forename: D C
authors.initials: DC
authors.lastname: Chan
authors.forename: S Y
authors.initials: SY
authors.lastname: Rodriguez
authors.forename: J H
authors.initials: JH
authors.lastname: Lintakoon
authors.forename: T H
authors.initials: TH
authors.lastname: Lam
mesh_terms: D002710:Chlorhexidine / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007202:Indicators and Reagents; D009967:Orthodontic Appliances; D012463:Saliva / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D007202:Indicators and Reagents; D002710:Chlorhexidine