Differentiation of the rat myelomonocytic leukemia cell line c-WRT-7 by in vitro culture with the rat bone marrow preadipocyte cell line REC A16.
Differentiation of the rat myelomonocytic leukemia cell line (c-WRT-7) was investigated, by co-culture with a rat embryonic bone marrow preadipose cell line (REC A16). Co-cultivation with REC A16, or with conditioned medium from REC A16 cultures (REC-CM), induced differentiation of c-WRT-7 cells to macrophages. A soluble factor(s) produced by REC A16 appeared to be responsible for the differentiation of c-WRT-7. Because REC-CM was associated with colony-stimulating activity on murine marrow progenitors, c-WRT-7 cells were cultured with various colony-stimulating factors (CSF) and it was found that macrophage CSF (M-CSF) significantly induced differentiation of c-WRT-7. We further demonstrated that both the colony-stimulating and differentiation-inducing activities of REC-CM were significantly blocked by anti-M-CSF antiserum. These results suggest that the differentiation of c-WRT-7 is due to M-CSF produced by REC A16. Co-culture of these two cell lines should provide a useful model to study the mechanisms of interaction between leukemia cells and marrow stroma.
Tanaka
T
T
Cancer Center, Kyushu University Hospital, Japan.
Okamura
S
S
Yasumoto
S
S
Takeichi
N
N
Kobayashi
H
H
Niho
Y
Y
D000818:Animals; D001854:Bone Marrow Cells; D002454:Cell Differentiation; D002455:Cell Division; D002461:Cell Line, Transformed; D002471:Cell Transformation, Neoplastic; D003115:Colony-Stimulating Factors / Q000276:immunology / Q000494:pharmacology; D017077:Culture Media, Conditioned; D006412:Hematopoietic Stem Cells / Q000166:cytology; D007375:Interleukin-1 / Q000494:pharmacology; D015479:Leukemia, Myelomonocytic, Acute / Q000473:pathology*; D051379:Mice; D008810:Mice, Inbred C57BL; D051381:Rats; D011994:Recombinant Proteins / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D003115:Colony-Stimulating Factors; D017077:Culture Media, Conditioned; D007375:Interleukin-1; D011994:Recombinant Proteins