A cancer-reactive human monoclonal antibody derived from a colonic cancer patient treated with local immunotherapy.
A human monoclonal antibody, YJ-37 (IgM) was generated through the fusion of human B lymphoblastoid cell line HO-323 with the regional lymph node lymphocytes from a colonic cancer patient who was treated with a local immunotherapy. This antibody was purified and conjugated with biotin, after which direct immunohistochemical staining was performed. The results revealed that YJ-37 selectively reacted with colonic cancer (7/19), gastric cancer (3/6), endometrial cancer (1/2) and colonic adenoma (7/13), but not with normal epithelia. Membrane immunofluorescence and FACS analysis also showed that YJ-37 bound to tumor cell surfaces. Furthermore, the chemical structure of the antigen defined by YJ-37 was analyzed by means of thin-layer chromatography immunostaining and ELISA. The results indicated that YJ-37 reacted with sialylated lacto-series carbohydrate chains, which have been reported to accumulate in cancer cells.
Yagyu
T
T
Second Department of Surgery, Osaka University Medical School.
Monden
T
T
Baba
M
M
Tamaki
Y
Y
Takeda
T
T
Kobayashi
T
T
Shimano
T
T
Tsuji
Y
Y
Matsushita
H
H
Osawa
H
H
D000236:Adenoma / Q000276:immunology / Q000473:pathology; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000302:isolation & purification*; D001402:B-Lymphocytes; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002459:Cell Fusion; D002460:Cell Line; D003110:Colonic Neoplasms / Q000276:immunology* / Q000473:pathology / Q000628:therapy; D016889:Endometrial Neoplasms / Q000276:immunology / Q000473:pathology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005340:Fibrinogen / Q000627:therapeutic use; D006017:Glycolipids / Q000032:analysis / Q000737:chemistry / Q000276:immunology; D006801:Humans; D007167:Immunotherapy; D008198:Lymph Nodes / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology*; D008969:Molecular Sequence Data; D010844:Picibanil / Q000627:therapeutic use*; D013274:Stomach Neoplasms / Q000276:immunology / Q000473:pathology
D000911:Antibodies, Monoclonal; D006017:Glycolipids; D010844:Picibanil; D005340:Fibrinogen