Frequent deletion of the transgene in T cell receptor beta chain transgenic mice.
TCR V beta 8.1 transgenic mice were generated using a genomic TCR V beta gene construct under the control of its promoter and enhancer. Among three lines of transgenic mice, one line expressed the transgenic TCR on only approximately 70% of peripheral T cells, while the other two lines expressed it on almost all mature T cells. T cells which lacked expression of the transgenic TCR beta chain expressed endogenous TCR beta chains. The molecular basis underlying the lack of transgene expression in T cells of this line of transgenic mice was investigated. The transgenic TCR- cells were isolated by two methods. First, Thy-1+ V beta 8.1/8.2- cells were purified from peripheral T cells using cell sorting. Second, transgenic TCR- T cell clones were established. In both cases, Southern blotting indicated that V beta 8.1- T cells had deleted the transgenic TCR gene. Thus, deletion of the transgenic TCR can occur in a high proportion of T cells, which allows rearrangement and expression of endogenous TCR beta chains.
Komori
S
S
Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia 19104-6082.
Katsumata
M
M
Greene
M I
MI
Yui
K
K
D000818:Animals; D001483:Base Sequence; D004252:DNA Mutational Analysis; D004742:Enhancer Elements, Genetic; D017353:Gene Deletion; D005813:Genes, Synthetic; D051379:Mice; D008822:Mice, Transgenic / Q000235:genetics*; D016747:Minor Lymphocyte Stimulatory Antigens / Q000235:genetics; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics*; D016176:T-Lymphocyte Subsets / Q000276:immunology
D016747:Minor Lymphocyte Stimulatory Antigens; D016693:Receptors, Antigen, T-Cell, alpha-beta