Resistance to cymbidium ringspot tombusvirus infection in transgenic Nicotiana benthamiana plants expressing the virus coat protein gene.
Transgenic Nicotiana benthamiana plants expressing the coat protein gene of cymbidium ringspot virus (CyRSV) were tested for resistance against infection with CyRSV. Transgenic plants showed resistance to infection only when the purified virions concentration in the inoculum was as low as 0.05 micrograms/ml. No protection was observed in transgenic plants inoculated with virion concentrations of 0.5 and 5.0 micrograms/ml or when the inoculum was in vitro synthesized genomic RNA.
Rubino
L
L
Dipartimento di Protezione delle Piante, Università degli Studi, Bari, Italy.
Capriotti
G
G
Lupo
R
R
Russo
M
M
D001483:Base Sequence; D015153:Blotting, Western; D002213:Capsid / Q000235:genetics* / Q000276:immunology; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000032:analysis; D015870:Gene Expression; D005814:Genes, Viral; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D010935:Plant Diseases / Q000235:genetics*; D010942:Plant Viruses / Q000472:pathogenicity*; D030821:Plants, Genetically Modified / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics; D015678:Viral Structural Proteins / Q000235:genetics
D004279:DNA, Viral; D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger; D015678:Viral Structural Proteins