title: Primary contribution of the injector to carryover of a trace analyte in high-performance liquid chromatography.
abstract: When a low-nanogram amount of N1,N3-bis-(pentafluorobenzyl)-N7-(2- [pentafluorobenzyloxy]ethyl)xanthine was subjected to HPLC, low-picogram amounts of the compound could be detected subsequently (off-line by gas chromatography-electron-capture negative-ion mass spectrometry) after injection of pure mobile phase. This was in spite of significant, intermediate washing of the injector and column. It was determined that essentially 99.9% of this analyte contamination came from the injector. Use of two injectors is a practical remedy for this problem.
authors.affiliation: Department of Pharmaceutical Sciences, College of Pharmacy and Allied Health Professions, Boston, MA.
authors.forename: M
authors.initials: M
authors.lastname: Saha
authors.forename: R W
authors.initials: RW
authors.lastname: Giese
mesh_terms: D016477:Artifacts; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000592:standards; D005464:Fluorobenzenes / Q000032:analysis*; D014970:Xanthines / Q000032:analysis*; D005466:Fluorocarbons
chemical_list: D005464:Fluorobenzenes; D014970:Xanthines; C079538:N1,N3-bis(pentafluorobenzyl)-N7-(2-(pentafluorobenzyloxy)ethyl)xanthine; D005466:Fluorocarbons