title: Determination of low-molecular-weight heparin by Heptest on the automated coagulation laboratory system.
abstract: The manual Heptest for measuring low-molecular-weight heparin fractions was applied to a fully automated, coagulation-dedicated analyzer, the Automated Coagulation Laboratory 300 Plus. The clot-based assay mode of the instrument was used, which operates on the principle of light scattering. Undiluted plasmas and the original reagents of the Heptest kit were used. Also, the 2-minute incubation time of the manual procedure was maintained. Automation reduced plasma and reagent volumes by about one half. As a result of the high precision of the automated procedure, single determinations suffice, and 18 plasma samples can be analyzed in about 8 minutes. Coefficients of variation were 1.0% to 3.2% for within-run and 1.9% to 6.0% for inter-run analyses. Analytical recovery was 98% to 104%. Comparisons of 132 samples between the two procedures yielded an R value of 0.974 for activity expression in seconds and 0.945 for U/mL. Several low-molecular-weight heparin fractions were tested.
authors.affiliation: Department of Pathology, Wayne State University School of Medicine, Detroit, Michigan.
authors.forename: T
authors.initials: T
authors.lastname: Ozawa
authors.forename: J
authors.initials: J
authors.lastname: Domagalski
authors.forename: E F
authors.initials: EF
authors.lastname: Mammen
mesh_terms: D001780:Blood Coagulation Tests / Q000295:instrumentation* / Q000592:standards; D003936:Diagnosis, Computer-Assisted; D006493:Heparin / Q000032:analysis* / Q000737:chemistry; D006801:Humans; D008970:Molecular Weight; D012016:Reference Values
chemical_list: D006493:Heparin