title: Post-transcriptional regulation of RNA polymerase II levels in Caenorhabditis elegans.
abstract: To investigate the regulation of RNA polymerase II levels in Caenorhabditis elegans, we have constructed nematode strains having one, two, or three copies of ama-1, the gene for the largest subunit of RNA polymerase II. Steady-state levels of RNA polymerase II polypeptides and solubilized enzyme activity are invariant with gene dosage, indicating regulatory compensation. However, steady-state levels of ama-1 mRNA are directly proportional to gene dosage. These results imply that RNA polymerase II levels in C. elegans are regulated post-transcriptionally.
authors.affiliation: Division of Biological Sciences University of Missouri-Columbia 65211.
authors.forename: B K
authors.initials: BK
authors.lastname: Dalley
authors.forename: T M
authors.initials: TM
authors.lastname: Rogalski
authors.forename: G E
authors.initials: GE
authors.lastname: Tullis
authors.forename: D L
authors.initials: DL
authors.lastname: Riddle
authors.forename: M
authors.initials: M
authors.lastname: Golomb
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000201:enzymology / Q000235:genetics*; D015971:Gene Expression Regulation, Enzymologic; D005796:Genes; D015801:Helminth Proteins / Q000096:biosynthesis* / Q000235:genetics; D011499:Protein Processing, Post-Translational; D012319:RNA Polymerase II / Q000096:biosynthesis* / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid
chemical_list: D015801:Helminth Proteins; D012319:RNA Polymerase II