Evaluation of Gen-Probe's Histoplasma capsulatum and Cryptococcus neoformans AccuProbes.
Gen-Probe's DNA probes were evaluated for use in the identification of clinical isolates of Histoplasma capsulatum var. capsulatum and Cryptococcus neoformans. Ninety-five mould-phase fungi were probed, including 41 isolates of H. capsulatum var. capsulatum. Similarly, 98 yeasts, including 42 C. neoformans isolates, were examined by using the C. neoformans DNA probe. In the study, both probes demonstrated 100% specificity and 100% sensitivity. Their use in the clinical laboratory may significantly reduce the time required for definitive identification of fungi.
Huffnagle
K E
KE
Department of Pathology, University of Texas Southwestern Medical Center, Dallas 75235-9072.
Gander
R M
RM
D003453:Cryptococcosis / Q000175:diagnosis; D003455:Cryptococcus neoformans / Q000235:genetics* / Q000302:isolation & purification; D015342:DNA Probes; D004271:DNA, Fungal / Q000235:genetics; D005069:Evaluation Studies as Topic; D006658:Histoplasma / Q000235:genetics* / Q000302:isolation & purification; D006660:Histoplasmosis / Q000175:diagnosis; D006801:Humans; D015336:Molecular Probe Techniques / Q000706:statistics & numerical data; D012680:Sensitivity and Specificity
D015342:DNA Probes; D004271:DNA, Fungal