title: Operator derepressed mutations in the proline utilisation gene cluster of Aspergillus nidulans.
abstract: The proline utilisation gene cluster of Aspergillus nidulans can be repressed efficiently only when both repressing nitrogen and repressing carbon sources are present. We show that two cis-acting mutations in this cluster permit the efficient transcription of the prnB gene under repressing conditions, resulting in direct or indirect derepression of two other transcripts of the pathway. These mutations are transitions that define a 5'GAGACCCC3' sequence. Similar sequences are found upstream of other genes subject to carbon catabolite repression. We propose that this sequence defines the binding site for the negatively-acting CreA protein, which mediates carbon catabolite repression in this fungus.
authors.affiliation: Institut de Génétique et Microbiologie, Unité Associée au CNRS 1354, Université Paris-Sud, Centre d'Orsay, France.
authors.forename: V
authors.initials: V
authors.lastname: Sophianopoulou
authors.forename: T
authors.initials: T
authors.lastname: Suárez
authors.forename: G
authors.initials: G
authors.lastname: Diallinas
authors.forename: C
authors.initials: C
authors.lastname: Scazzocchio
mesh_terms: D000595:Amino Acid Sequence; D001233:Aspergillus nidulans / Q000235:genetics*; D001483:Base Sequence; D002244:Carbon / Q000378:metabolism; D003001:Cloning, Molecular; D004794:Enzyme Repression; D005656:Fungal Proteins / Q000378:metabolism; D005800:Genes, Fungal / Q000235:genetics*; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics; D009154:Mutation; D009584:Nitrogen / Q000378:metabolism; D009875:Operator Regions, Genetic / Q000235:genetics*; D011392:Proline / Q000378:metabolism*; D012322:RNA Precursors / Q000032:analysis; D012097:Repressor Proteins / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D005656:Fungal Proteins; D012322:RNA Precursors; D012097:Repressor Proteins; C071237:CreA protein, Aspergillus nidulans; D002244:Carbon; D011392:Proline; D009584:Nitrogen