High-performance liquid chromatography of imipramine and six metabolites in human plasma and urine.
A method for the simultaneous quantitation of imipramine and six metabolites (2- and 10-hydroxyimipramine, 2- and 10-hydroxydesipramine, didesmethylimipramine and desipramine) in human plasma and urine has been developed. The method is based on a three-step liquid-liquid extraction followed by isocratic, reversed-phase high-performance liquid chromatography with ultraviolet absorbance detection (detection wavelength: 220 nm). The chromatographic eluent consisted of 30% acetonitrile and 70% aqueous sodium perchlorate solution pH 2.5. Glucuronide conjugates in urine were deconjugated with beta-glucuronidase/arylsulphatase prior to extraction.
Nielsen
K K
KK
Department of Clinical Pharmacology, Institute of Medical Biology, Odense University, Denmark.
Brøsen
K
K
D001192:Arylsulfatases; D002851:Chromatography, High Pressure Liquid; D003891:Desipramine / Q000032:analysis / Q000097:blood / Q000652:urine; D005966:Glucuronidase; D006801:Humans; D007099:Imipramine / Q000032:analysis* / Q000097:blood / Q000652:urine; D012015:Reference Standards
D001192:Arylsulfatases; D005966:Glucuronidase; D007099:Imipramine; D003891:Desipramine