Human gamma-interferon expression in the mammary gland of transgenic mice.
Transgenic mice carrying a hybrid gene consisting of ovine beta-lactoglobulin gene sequences and human gamma-interferon (hIFN-g) cDNA were produced. hIFN-g expression in the mammary gland of two lactating transgenic founder females was found. The concentration of active hIFN-g in the milk was estimated as being ca. 1800 IU/ml. The hIFN-g ability to express in the mammary gland was found in the progeny of transgenic founder male.
Dobrovolsky
V N
VN
Shemyakin Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow.
Lagutin
O V
OV
Vinogradova
T V
TV
Frolova
I S
IS
Kuznetsov
V P
VP
Larionov
O A
OA
D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000235:genetics; D005260:Female; D015870:Gene Expression; D006801:Humans; D007371:Interferon-gamma / Q000032:analysis / Q000235:genetics*; D007774:Lactation; D007782:Lactoglobulins / Q000235:genetics; D008321:Mammary Glands, Animal / Q000737:chemistry / Q000378:metabolism*; D051379:Mice; D008822:Mice, Transgenic; D008892:Milk / Q000737:chemistry; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis; D011993:Recombinant Fusion Proteins / Q000235:genetics
D007782:Lactoglobulins; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D007371:Interferon-gamma; D004247:DNA