Biodegradable microparticles for oral immunization.
Ovalbumin (OVA) was entrapped in poly(lactide-co-glycolide) microparticles and administered to mice. Following intraperitoneal immunization, the microparticles induced both proliferative T-cell responses and cytotoxic T-cell responses in spleen cells. Following oral immunization, the mean salivary IgA antibody response to microparticles was significantly greater than the response to soluble OVA (p < 0.0001). Serum IgG antibody levels were also significantly greater in the group administered microparticles (p < 0.001). Cholera toxin B subunit was also entrapped in microparticles. Following oral immunization in mice, specific antibody-secreting cells were detected both in the spleens and in the mesenteric lymph nodes.
O'Hagan
D T
DT
Department of Pharmaceutical Sciences, University of Nottingham, University Park, UK.
McGee
J P
JP
Holmgren
J
J
Mowat
A M
AM
Donachie
A M
AM
Mills
K H
KH
Gaisford
W
W
Rahman
D
D
Challacombe
S J
SJ
D000284:Administration, Oral; D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D001673:Biodegradation, Environmental; D002772:Cholera Toxin / Q000008:administration & dosage / Q000276:immunology*; D003692:Delayed-Action Preparations; D004339:Drug Compounding; D004655:Emulsions; D007071:Immunoglobulin A, Secretory / Q000096:biosynthesis* / Q000276:immunology; D007074:Immunoglobulin G / Q000096:biosynthesis* / Q000097:blood / Q000276:immunology; D007274:Injections, Intraperitoneal; D008198:Lymph Nodes / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology; D008863:Microspheres; D010047:Ovalbumin / Q000008:administration & dosage / Q000276:immunology*; D011098:Polyglactin 910 / Q000493:pharmacokinetics; D013154:Spleen / Q000276:immunology; D016176:T-Lymphocyte Subsets / Q000276:immunology*
D000914:Antibodies, Viral; D003692:Delayed-Action Preparations; D004655:Emulsions; D007071:Immunoglobulin A, Secretory; D007074:Immunoglobulin G; D011098:Polyglactin 910; D010047:Ovalbumin; D002772:Cholera Toxin