title: Requirement for Cdk2 in cytostatic factor-mediated metaphase II arrest.
abstract: The unfertilized eggs of vertebrates are arrested in metaphase of meiosis II because of the activity of cytostatic factor (CSF). Xenopus CSF is thought to contain the product of the Mos proto-oncogene, but other proteins synthesized during meiosis II are also required for arrest induced by CSF. In Xenopus oocytes, ablation of synthesis of cyclin-dependent kinase 2 (Cdk2) during meiosis resulted in absence of the metaphase II block, even though the Mosxe protein kinase was fully active at metaphase. Introduction of purified Cdk2 restored metaphase II arrest, and increasing the amount of Cdk2 during meiosis I (when Mosxe is present) led to metaphase arrest at meiosis I. These data indicate that metaphase arrest is a result of cooperation between a proto-oncogene kinase and a cyclin-dependent kinase and illustrate the interaction of a cell growth regulator with a cell cycle control element.
authors.affiliation: Department of Pharmacology, University of Colorado School of Medicine, Denver 80262.
authors.forename: B G
authors.initials: BG
authors.lastname: Gabrielli
authors.forename: L M
authors.initials: LM
authors.lastname: Roy
authors.forename: J L
authors.initials: JL
authors.lastname: Maller
mesh_terms: D000818:Animals; D001483:Base Sequence; D042846:CDC2-CDC28 Kinases; D051357:Cyclin-Dependent Kinase 2; D018844:Cyclin-Dependent Kinases; D005260:Female; D008540:Meiosis / Q000502:physiology*; D008677:Metaphase / Q000502:physiology*; D008969:Molecular Sequence Data; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D009865:Oocytes / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D010766:Phosphorylation; D011061:Poly A / Q000378:metabolism; D011374:Progesterone / Q000494:pharmacology; D011494:Protein Kinases / Q000235:genetics / Q000502:physiology*; D017346:Protein Serine-Threonine Kinases; D017231:Proto-Oncogene Proteins c-mos / Q000378:metabolism / Q000502:physiology*; D012333:RNA, Messenger / Q000378:metabolism; D014981:Xenopus; D029867:Xenopus Proteins
chemical_list: D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger; D029867:Xenopus Proteins; D011061:Poly A; D011374:Progesterone; D011494:Protein Kinases; D017346:Protein Serine-Threonine Kinases; D017231:Proto-Oncogene Proteins c-mos; D042846:CDC2-CDC28 Kinases; C495891:Cdk2 protein, Xenopus; D051357:Cyclin-Dependent Kinase 2; D018844:Cyclin-Dependent Kinases