Differential effects of fluoride and a non-hydrolysable GTP analogue on adenylate cyclase and G-proteins in Ceratitis capitata neural tissue.
We have examined the effects of fluoride on guanine nucleotide-binding regulatory proteins (G-proteins) in neural membranes from the dipterous Ceratitis capitata. Fluoride effects on the Gs-protein were monitored by determining adenylate cyclase activity and cholera toxin-catalysed ADP-ribosylation whereas those on the G(o)-protein were studied by measuring ADP-ribosylation with pertussis toxin. Data are discussed in relation to the effects of a non-hydrolysable GTP analogue. G-protein activation carried out by fluoride seems not to mimic, at least in insects, activation by non-hydrolysable GTP analogues, in opposition to that proposed for transducin, the G-protein of the mammalian visual system, and other G-proteins.
Guillén
A
A
Departamento de Bioquímica y Biología Molecular I, Facultad de Ciencias Químicas, Universidad Complutense, Madrid, Spain.
Homburger
V
V
Pérez-Baun
J C
JC
Haro
A
A
D000246:Adenosine Diphosphate Ribose / Q000378:metabolism; D037361:Adenylate Cyclase Toxin; D000262:Adenylyl Cyclases / Q000187:drug effects* / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D002772:Cholera Toxin / Q000378:metabolism; D004175:Diptera / Q000378:metabolism*; D019204:GTP-Binding Proteins / Q000187:drug effects* / Q000378:metabolism; D006165:Guanylyl Imidodiphosphate / Q000494:pharmacology*; D006868:Hydrolysis; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000187:drug effects* / Q000378:metabolism; D010206:Papain / Q000494:pharmacology; D037342:Pertussis Toxin; D010741:Phospholipases A / Q000494:pharmacology; D011402:Pronase / Q000494:pharmacology; D015398:Signal Transduction / Q000187:drug effects; D012969:Sodium Fluoride / Q000494:pharmacology*; D013381:Subtilisins / Q000494:pharmacology; D010566:Virulence Factors, Bordetella / Q000378:metabolism
D037361:Adenylate Cyclase Toxin; D009419:Nerve Tissue Proteins; D010566:Virulence Factors, Bordetella; D000246:Adenosine Diphosphate Ribose; D006165:Guanylyl Imidodiphosphate; D012969:Sodium Fluoride; D002772:Cholera Toxin; D037342:Pertussis Toxin; D010741:Phospholipases A; D013381:Subtilisins; D010206:Papain; D011402:Pronase; D019204:GTP-Binding Proteins; D000262:Adenylyl Cyclases