Isolation and purification of a new glycoprotein from human urine inhibiting calcium oxalate crystallization.
A calcium oxalate crystal growth inhibitor was isolated from human urine using DEAE-Sephacel gel followed by Sephacryl S-300 chromatography and FPLC column. The isolated inhibitor was a uronic-acid-rich protein (UAP). It was found to be a glycoprotein with a molecular weight of 35,000 Da as determined by SDS-polyacrylamide gel electrophoresis. Inhibitory activity was demonstrated using a calcium oxalate crystallization system. In addition UAP, nephrocalcin (NC) or nephrocalcin-like (NC-like) activity was an effective inhibitor in this system. However, the inhibitory activity of UAP appeared to be higher than that of NC or NC-like activity. This finding suggests that NC or NC-like activity is not only urinary protein with strong inhibitory activity. UAP and probably other proteins also play a role in the control of urinary crystal growth.
Atmani
F
F
Département de Néphrologie, Hôpital-Necker Enfants Malades, Paris, France.
Lacour
B
B
Drüeke
T
T
Daudon
M
M
D002129:Calcium Oxalate / Q000737:chemistry / Q000652:urine*; D002845:Chromatography / Q000379:methods; D003460:Crystallization; D006023:Glycoproteins / Q000737:chemistry / Q000302:isolation & purification / Q000652:urine*; D006801:Humans; D007669:Kidney Calculi / Q000209:etiology / Q000652:urine; D008297:Male; D008970:Molecular Weight; D014574:Uronic Acids / Q000032:analysis
D006023:Glycoproteins; D014574:Uronic Acids; D002129:Calcium Oxalate