New patterns of diacylglycerol metabolism in intact cells.
The metabolism of di[1-14C]octanoylglycerol metabolism was examined in four cell lines: NIH 3T3 fibroblasts, BHK cells, Pam 212 keratinocytes and WEHI 3BD+ cells. We found the direct conversion of 1,2-di[1-14C]octanoyl-sn-glycerol ([14C]diC8) into dioctanoylphosphatidylcholine and dioctanoylacylglycerol, but no formation of phosphatidylinositol. The [14C]diC8 also underwent lipolytic breakdown. In contrast, 1-[1-14C]oleoyl-2-acetyl-sn-glycerol was metabolized exclusively by lipolysis. Our findings support a new scheme for the metabolic termination of diacylglycerol signals.
Florin-Christensen
J
J
Department of Internal Medicine, Yale University, New Haven, CT 06510.
Florin-Christensen
M
M
Delfino
J M
JM
Rasmussen
H
H
D016475:3T3 Cells; D000818:Animals; D002463:Cell Membrane Permeability; D003512:Cyclohexanones / Q000494:pharmacology; D004075:Diglycerides / Q000378:metabolism*; D008055:Lipids / Q000302:isolation & purification; D008066:Lipolysis; D008071:Lipoprotein Lipase / Q000037:antagonists & inhibitors; D051379:Mice; D010713:Phosphatidylcholines / Q000378:metabolism; D010716:Phosphatidylinositols / Q000378:metabolism; D014407:Tumor Cells, Cultured
D003512:Cyclohexanones; D004075:Diglycerides; D008055:Lipids; D010713:Phosphatidylcholines; D010716:Phosphatidylinositols; C044295:1,2-dioctanoylglycerol; C036535:1,6-bis(cyclohexyloximinocarbonyl)hexane; C038237:1-oleoyl-2-acetylglycerol; D008071:Lipoprotein Lipase