Characterization of a variant of the polysaccharide acetan produced by a mutant of Acetobacter xylinum strain CR1/4.
Acetobacter xylinum NRRL B42 (NCIB 40123) produces both cellulose and a complex anionic branched heteropolysaccharide called acetan. Chemical mutagenesis was used to isolate stable cellulose-minus Acetobacter xylinum mutants. Further chemical mutagenesis of these cellulose-minus A. xylinum bacteria was used to select mutants which secrete polysaccharides which are variants of the acetan structure. Preparation, purification and characterization of these polysaccharides are described. Methylation analysis of the polysaccharide structure CR1/4 suggests that the polysaccharide has an acetan structure with a truncated sidechain terminating in glucuronic acid.
MacCormick
C A
CA
AFRC Institute of Food Research, Norwich Laboratory, Norwich Research Park, Colney, UK.
Harris
J E
JE
Gunning
A P
AP
Morris
V J
VJ
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000032:analysis; D041822:Gluconacetobacter xylinus / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D008745:Methylation; D008769:Methylnitronitrosoguanidine; D008969:Molecular Sequence Data; D016296:Mutagenesis; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification
D002241:Carbohydrates; D011135:Polysaccharides, Bacterial; C068545:acetan; D008769:Methylnitronitrosoguanidine