Binding of lead to a metallothionein-like protein in human erythrocytes.
We have studied the erythrocytes from 24 workers occupationally exposed to inorganic lead, one asymptomatic lead worker showing exceptionally high exposure, and eight control subjects (blood lead 300-750, 1800, and < 100 micrograms/L, respectively). High performance protein chromatography, electrophoresis, and trace metal analysis have identified a low M.Wt., copper, and zinc-containing protein in all cases. This protein (designated protein M) bound lead on in vitro incubation with buffered lead nitrate. Purified samples of protein M were found to show characteristics consistent with metallothionein (M.Wt. approximately 6500, low pI, and greater UV absorbance at 254 nm). Amino acid analysis found a composition of 33% cysteine but no aromatic amino acids. The highly exposed subject showed endogenous lead binding to protein M, which on further purification by ion exchange was found to be associated with one particular constituent (protein M5). Protein M5 was present in much lower quantities in control subjects. These findings suggest the existence of a metallothionein-like protein in erythrocytes which binds lead, sequestering it into a nonbioavailable form and hence protects against lead toxicity.
Church
H J
HJ
Department of Chemistry, University of Manchester, U.K.
Day
J P
JP
Braithwaite
R A
RA
Brown
S S
SS
D000328:Adult; D002352:Carrier Proteins / Q000097:blood / Q000302:isolation & purification; D004912:Erythrocytes / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D007526:Isoelectric Point; D007854:Lead / Q000097:blood*; D007855:Lead Poisoning / Q000097:blood; D008297:Male; D008668:Metallothionein / Q000097:blood* / Q000302:isolation & purification; D008970:Molecular Weight; D009784:Occupational Diseases / Q000097:blood; D016273:Occupational Exposure; D011485:Protein Binding
D002352:Carrier Proteins; C055217:lead-binding proteins; D007854:Lead; D008668:Metallothionein