A method for spatio-temporal (4-D) data representation in confocal microscopy: application to neuroanatomical plasticity.
This paper describes a new method for data representation and visualization in four dimensions (three dimensions plus time). Sequential volumes, exhibiting morphological activity, are acquired non-invasively with a confocal scanning laser microscope, where each data set corresponds to a time sample. A pipelined processing includes packing of volumes and specific volume rendering techniques. Subsequent processing in HIS (hue, intensity, saturation)l colour space combines functional, packed images with shaded three-dimensional views. As a result, even subtle changes in morphology become visible and computational time is saved. Experimental findings obtained from investigations of synaptic plasticity in cultured retinal tissue are reported.
Kriete
A
A
Institut für Anatomie und Zytobiologie, Justus-Liebig-Universität Giessen, Germany.
Wagner
H J
HJ
D000465:Algorithms; D000818:Animals; D003118:Color Perception; D046508:Culture Techniques; D003623:Dark Adaptation; D005399:Fishes; D007091:Image Processing, Computer-Assisted; D008027:Light; D008856:Microscopy, Fluorescence / Q000379:methods*; D010786:Photoreceptor Cells / Q000502:physiology; D012160:Retina / Q000033:anatomy & histology* / Q000502:physiology; D013569:Synapses / Q000502:physiology / Q000648:ultrastructure*