title: Analysis and bioequivalency study on two tablet formulations of co-trimoxazole.
abstract: This paper describes an analytical procedure for simultaneous quantification of trimethoprim (TMP) and sulphamethoxazole (SMZ) in serum. Serum samples were rendered alkaline with glycine-sodium hydroxide buffer (pH 9.1) and extracted with ethyl acetate. The reconstituted sample was analysed using reversed-phase C-18 column high-performance chromatography. The mobile phase consisted of 75% triethyl ammonium acetate buffer, 20% methanol and 5% acetonitrile. The analytes were monitored at 289 nm and tetroxoprim was used as an internal standard. The maximum values for intra-day coefficients of variation (CV) for TMP-and SMZ were 5.7 and 2.1%, respectively. Interday CV values were 6.4 and 2.2%, respectively. The method was used to compare the bioavailability of two tablet formulations in terms of their pharmacokinetic parameters following oral administration of the tablets to 18 volunteers.
authors.affiliation: Faculty of Pharmacy, Jordan University of Science and Technology, Irbid.
authors.forename: M
authors.initials: M
authors.lastname: Sheikh Salem
authors.forename: H N
authors.initials: HN
authors.lastname: Alkaysi
authors.forename: A M
authors.initials: AM
authors.lastname: Gharaibeh
authors.forename: F F
authors.initials: FF
authors.lastname: Amari
authors.forename: E
authors.initials: E
authors.lastname: Sallam
authors.forename: M S
authors.initials: MS
authors.lastname: Shubair
mesh_terms: D001682:Biological Availability; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D008297:Male; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013607:Tablets; D013810:Therapeutic Equivalency; D015662:Trimethoprim, Sulfamethoxazole Drug Combination / Q000097:blood* / Q000493:pharmacokinetics*
chemical_list: D013607:Tablets; D015662:Trimethoprim, Sulfamethoxazole Drug Combination