Covalent linking of haptens, proteins and nucleic acids to a modified polystyrene support.
Biological molecules of various molecular weights were successfully linked to polystyrene microtitration plates bearing carbonyl and hydroxyl reactive groups. Cross-linking of proteins, nucleic acids and haptens was carried out with water soluble carbodiimide. The covalent nature of the binding reaction was demonstrated by the immunodetection of a hapten linked to the surface through a disulphide spacer arm and its subsequent release by cleavage of the bridge. The amount of protein fixed per surface unit could be correlated to molecular weight. Nanograms of biotinylated nucleic acids and synthetic polynucleotides could also be retained on the solid support.
Niveleau
A
A
Laboratoire de Génie Enzymatique, Université Claude Bernard, Villeurbanne, France.
Sage
D
D
Reynaud
C
C
Bruno
C
C
Legastelois
S
S
Thomas
V
V
Dante
R
R
D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006241:Haptens / Q000032:analysis; D009696:Nucleic Acids / Q000032:analysis; D011137:Polystyrenes; D011506:Proteins / Q000032:analysis
D006241:Haptens; D009696:Nucleic Acids; D011137:Polystyrenes; D011506:Proteins