Determination of malonaldehyde in human plasma: elimination of spectral interferences in the 2-thiobarbituric acid reaction.
A selective, derivative spectrophotometric method has been developed for the determination of malonaldehyde (MLD), based on a reaction with 2-thiobarbituric acid (TBA). The proposed method has been applied to the determination of MLD in human plasma. A study to eliminate several spectral interferences is described. A comparative study of the results obtained using the proposed derivative method and a conventional TBA method applied to human plasma is presented and the advantages of the proposed method over the conventional method for the determination of MLD in human plasma are evaluated.
Espinosa-Mansilla
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Department of Analytical Chemistry, University of Extremadura, Badajoz, Spain.
Salinas
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F
Leal
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AR
D006801:Humans; D008315:Malondialdehyde / Q000097:blood*; D013053:Spectrophotometry / Q000379:methods; D013858:Thiobarbiturates
D013858:Thiobarbiturates; D008315:Malondialdehyde; C029684:thiobarbituric acid