Error analysis in manual and automated DNA sequencing.
The frequency, position, and type of errors were analyzed for sequence data which were generated with the help of (I) a manual digitizer, (II) an off-line automated film reader, and (III) an on-line automated gel reader. About 400 film/gel readings were analyzed and the results were used to generate error profiles for each method. The profiles identify several method- and project-specific problem areas and provide useful guidance for the assessment of DNA sequence data in general.
Khurshid
F
F
Imperial Cancer Research Fund, London.
Beck
S
S
D005069:Evaluation Studies as Topic; D006801:Humans; D015203:Reproducibility of Results; D017422:Sequence Analysis, DNA / Q000379:methods* / Q000706:statistics & numerical data