Newly acquired peri-telomeric heterochromatin in a transgenic mouse lineage.
Karyotypes of an embryonic stem cell line and normal bone marrow and peripheral blood cells from a transgenic mouse lineage, strain 83, exhibit a novel region of constitutive heterochromatin in the peri-telomeric region of chromosome 3. This heterochromatic region co-localizes with the integration site of the transgene, an 11-Mb tandem insertion of a plasmid containing human beta-globin gene sequences, and is inherited in Mendelian fashion. The significance of this finding is discussed, and a nomenclature for such newly acquired heterochromatic regions is suggested.
Simon
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Wistar Institute of Anatomy and Biology, Philadelphia, PA 19104.
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D000818:Animals; D002460:Cell Line; D002871:Chromosome Banding; D002875:Chromosomes / Q000737:chemistry / Q000648:ultrastructure*; D005260:Female; D006570:Heterochromatin / Q000648:ultrastructure*; D007621:Karyotyping; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D010375:Pedigree; D013234:Stem Cells / Q000648:ultrastructure; D016615:Telomere / Q000648:ultrastructure*
D006570:Heterochromatin