Transport of prepro-albumin into inverted vesicles prepared from the inner membrane of rat liver mitochondria.
When inverted vesicles prepared from the inner membrane of rat liver mitochondria were incubated with prepro-rat serum albumin, considerable amounts of prepro-albumin and pro-albumin were recovered with the inverted vesicles re-isolated by centrifugation. Pro-albumin was resistant to trypsin, but prepro-albumin was completely digested by trypsin, indicating that prepro-albumin was transported into the vesicles and concomitantly converted to pro-albumin. This transport process required ATP, but not a membrane potential. These results suggest that some export machinery for a protein having an amino acid sequence in its N-terminal portion similar to the signal sequence of secretory protein exists in the inner mitochondrial membrane.
Ono
H
H
Department of Biochemistry, Yamagata University School of Medicine, Japan.
Yoshida
T
T
Tuboi
S
S
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D002458:Cell Fractionation; D003902:Detergents / Q000494:pharmacology; D007425:Intracellular Membranes / Q000378:metabolism*; D008081:Liposomes / Q000378:metabolism*; D008564:Membrane Potentials; D008862:Microsomes, Liver / Q000378:metabolism; D008930:Mitochondria, Liver / Q000648:ultrastructure*; D011228:Prealbumin / Q000378:metabolism*; D011498:Protein Precursors / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D051381:Rats; D012995:Solubility; D014357:Trypsin / Q000378:metabolism
D003902:Detergents; D008081:Liposomes; D011228:Prealbumin; D011498:Protein Precursors; C014771:preproalbumin; D000255:Adenosine Triphosphate; D014357:Trypsin