Expression and functional characterization of a rat sulfotransferase (ST1A1) cDNA for sulfations of phenolic substrates in COS-1 cells.
A cDNA (PST-1) isolated from a rat liver cDNA library was expressed in COS-1 cells and found to encode a form of arylsulfotransferase (termed ST1A1), which sulfated p-nitrophenol, alpha-naphthol, minoxidil, beta-estradiol and dopamine, but not dehydroepiandrosterone and cortisol. ST1A1 showed a mobility identical with a major immunodetectable sulfotransferase contained in the livers of male and female rats in Western blots. In addition, ST1A1 mRNA was detected in the liver and extrahepatic tissues by Northern blots.
Ozawa
S
S
Department of Pharmacology, School of Medicine, Keio University, Tokyo, Japan.
Nagata
K
K
Gong
D W
DW
Yamazoe
Y
Y
Kato
R
R
D000818:Animals; D015152:Blotting, Northern; D015153:Blotting, Western; D002460:Cell Line; D004247:DNA / Q000032:analysis / Q000096:biosynthesis*; D005260:Female; D015723:Gene Library; D066298:In Vitro Techniques; D008099:Liver / Q000201:enzymology; D008297:Male; D010636:Phenols / Q000378:metabolism*; D051381:Rats; D013431:Sulfates / Q000378:metabolism; D015238:Sulfotransferases / Q000096:biosynthesis*
D010636:Phenols; D013431:Sulfates; D004247:DNA; D015238:Sulfotransferases