Degradation of 2-chloroallylalcohol by a Pseudomonas sp.
Three Pseudomonas strains capable of utilizing 2-chloroallylalcohol (2-chloropropenol) as the sole carbon source for growth were isolated from soil. The fastest growth was observed with strain JD2, with a generation time of 3.6 h. Degradation of 2-chloroallylalcohol was accompanied by complete dehalogenation. Chloroallylalcohols that did not support growth were dechlorinated by resting cells; the dechlorination level was highest if an alpha-chlorine substituent was present. Crude extracts of strain JD2 contained inducible alcohol dehydrogenase activity that oxidized mono- and dichloroallylalcohols but not trichloroallylalcohol. The enzyme used phenazine methosulfate as an artificial electron acceptor. Further oxidation yielded 2-chloroacrylic acid. The organism also produced hydrolytic dehalogenases converting 2-chloroacetic acid and 2-chloropropionic acid.
van der Waarde
J J
JJ
Department of Biochemistry, University of Groningen, The Netherlands.
Kok
R
R
Janssen
D B
DB
D000426:Alcohol Dehydrogenase / Q000378:metabolism*; D000498:Allyl Compounds / Q000378:metabolism*; D001673:Biodegradation, Environmental; D010084:Oxidation-Reduction; D011549:Pseudomonas / Q000145:classification / Q000201:enzymology / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*; D012988:Soil Microbiology
D000498:Allyl Compounds; C079708:2-chloroallyl alcohol; D000426:Alcohol Dehydrogenase