Humoral stimulating activities in post-cyclophosphamide rat sera and their purified fractions.
Enhanced humoral, stimulating activities (HSAs) of post-cyclophosphamide (CY) sera and their purified fractions, acting on mitogen activated T-lymphocytes, were detected in Wistar rats after treatment by high single doses of the aplasia producing alkylating cytostatic drug CY. These activities, monitored in vitro, were partially purified from post-CY sera, collected 2, 4, and 7 days after treatment, yielding fractions with higher humoral stimulating activity. The preliminary purification included molecular cutting by Amicon-Diaflo filters (1-30 kDa pore size range) and gel-filtration on Sephadex G-50 and G-75. Results show that post-CY sera partially purified fraction (10-20 kDa), enhances the proliferation of hydrocortisone resistant (HCR) T-lymphocytes up to threefold under microculture conditions (P << 0.001); partially purified post-CY sera fraction (10-20 kDa) increases the proliferative response of T-cells in microcultures to the mitogenic 145-2C11 monoclonal antibody (mAb) up to tenfold (P << 0.001). These results show that the activity of stimulating factors is localized in the molecular weight range of 10-20 kDa.
Pujic
N
N
Laboratory for Radiology and Radiation Medicine, Institute of Nuclear Sciences, Vinca, Belgrade, Yugoslavia.
Hegedis
L
L
Pavelic
K
K
Casl
T
T
Marusic
S
S
Savovski
K
K
Dujic
A
A
Dimitrijevic
B
B
D000818:Animals; D001798:Blood Proteins / Q000302:isolation & purification* / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects*; D002478:Cells, Cultured / Q000187:drug effects; D003520:Cyclophosphamide / Q000097:blood / Q000494:pharmacology*; D004351:Drug Resistance; D006854:Hydrocortisone; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008970:Molecular Weight; D051381:Rats; D017208:Rats, Wistar; D013154:Spleen / Q000187:drug effects; D013601:T-Lymphocytes / Q000187:drug effects*; D013936:Thymidine / Q000378:metabolism*; D013950:Thymus Gland / Q000187:drug effects
D001798:Blood Proteins; D003520:Cyclophosphamide; D013936:Thymidine; D006854:Hydrocortisone