title: Near-lifetime exposure of the rat to a simulated urban profile of nitrogen dioxide: pulmonary function evaluation.
abstract: To investigate the potential for up to a near-lifetime exposure to high-ambient levels of nitrogen dioxide (NO2) to induce functional lung damage, groups of rats were exposed to air or a simulated urban profile of NO2 (0.5 ppm background, 1.5 ppm peak) for 1, 3, 13, 52, or 78 weeks. The dynamic, static, and diffusional characteristics of the lung were evaluated postexposure in anesthetized rats. Furthermore, for the 13-, 52-, and 78-week groups, additional animals were tested after a 6-, 26-, or 17-week period in filtered air, respectively. No significant NO2 differences between exposed and control animals were found for the nitrogen washout, compliance, lung volume, or diffusion capacity of carbon monoxide measurements. At 78 weeks, however, a reduction in delta FEF25%, an estimate of convexity in the later portion of the forced expiratory flow volume curve, was observed. Breathing patterns and mechanisms were also assessed postexposure in a parallel group of similarly exposed unanesthetized rats. These rats were examined during a filtered air, 4 and 8% carbon dioxide (CO2) challenge. In the unanesthetized rat, frequency of breathing was significantly decreased and tidal volume, expiratory resistance, and inspiratory and expiratory times tended to increase. For several of these variables, the largest response also occurred at 78 weeks and seemed to be exacerbated by CO2 challenge.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: ManTech Environmental, Inc., Research Triangle Park, North Carolina 27709.
authors.forename: J S
authors.initials: JS
authors.lastname: Tepper
authors.forename: D L
authors.initials: DL
authors.lastname: Costa
authors.forename: D W
authors.initials: DW
authors.lastname: Winsett
authors.forename: M A
authors.initials: MA
authors.lastname: Stevens
authors.forename: D L
authors.initials: DL
authors.lastname: Doerfler
authors.forename: W P
authors.initials: WP
authors.lastname: Watkinson
mesh_terms: D000393:Air Pollutants / Q000633:toxicity*; D000818:Animals; D004562:Electrocardiography / Q000187:drug effects; D008171:Lung Diseases / Q000139:chemically induced* / Q000503:physiopathology; D008297:Male; D009585:Nitrogen Dioxide / Q000633:toxicity*; D051381:Rats; D011916:Rats, Inbred F344; D012129:Respiratory Function Tests; D015656:Respiratory Mechanics / Q000187:drug effects
chemical_list: D000393:Air Pollutants; D009585:Nitrogen Dioxide