title: Some properties of restriction endonuclease ApaBI from Acetobacter pasteurianus.
abstract: A new site-specific endonuclease has been isolated from Acetobacter pasteurianus and has been named ApaBI. The enzyme recognizes 35 cleavage sites on bacteriophage lambda DNA, 20 sites on adenovirus-2 DNA and 2 sites on plasmid pBR322. The recognition sequence for this enzyme is 3'-CGT/NNNNNACG-5' 5'-GCANNNNN/TGC-3'.
authors.affiliation: Department of Biochemistry, Faculty of Natural Science, Comenius University, Bratislava Slovak Republic.
authors.forename: J
authors.initials: J
authors.lastname: Grones
authors.forename: J
authors.initials: J
authors.lastname: Turna
mesh_terms: D000091:Acetobacter / Q000201:enzymology*; D001483:Base Sequence; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D004279:DNA, Viral / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000302:isolation & purification / Q000378:metabolism*; D008969:Molecular Sequence Data; D010957:Plasmids; D013379:Substrate Specificity
chemical_list: D004279:DNA, Viral; D015252:Deoxyribonucleases, Type II Site-Specific; C080472:GCANNNNNTGC -specific type II deoxyribonucleases