title: Function of periplasmic copper-zinc superoxide dismutase in Caulobacter crescentus.
abstract: Caulobacter crescentus is one of a small number of bacterial species that contain a periplasmic copper-zinc superoxide dismutase (CuZnSOD). A C. crescentus mutant, with the CuZnSOD gene interrupted by a promoterless cat gene, was constructed and characterized to analyze CuZnSOD function. Periplasmic SOD does not protect against oxyradical damage in the cytosol or play a major role in maintaining the integrity of the cell envelope. Studies of the effect of sodium citrate on plating efficiency suggest that CuZnSOD protects a periplasmic or membrane function(s) requiring magnesium or calcium.
authors.affiliation: Department of Biochemistry, Albert Einstein College of Medicine, Bronx, New York 10461.
authors.forename: H M
authors.initials: HM
authors.lastname: Steinman
mesh_terms: D002413:Cations, Divalent; D016934:Caulobacter / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D002462:Cell Membrane / Q000502:physiology; D002746:Chlorpromazine / Q000494:pharmacology; D002951:Citrates / Q000494:pharmacology; D005798:Genes, Bacterial; D016254:Mutagenesis, Insertional; D010084:Oxidation-Reduction; D010269:Paraquat / Q000494:pharmacology; D013482:Superoxide Dismutase / Q000378:metabolism*
chemical_list: D002413:Cations, Divalent; D002951:Citrates; D013482:Superoxide Dismutase; D010269:Paraquat; D002746:Chlorpromazine