Analysis of the neurotoxin beta-ODAP and its alpha-isomer by precolumn derivatization with phenylisothiocyanate.
A method is presented for determining 3-N-oxalyl-2,3-diaminopropanoic acid (beta-ODAP), the most potent neurotoxic substance of the seeds and seedlings of Lathyrus sativus, and its much less toxic 2-isomer (alpha-ODAP). The separation of the two forms is achieved after derivatization with phenylisothiocyanate employing a HPLC system. This method was used to monitor the isomerization of beta-ODAP to alpha-ODAP at different time intervals and to quantify the toxin level in seed extracts.
Khan
J K
JK
Laboratory of Physiological Chemistry, Faculty of Medicine, University of Ghent, Belgium.
Kebede
N
N
Kuo
Y H
YH
Lambein
F
F
De Bruyn
A
A
D000599:Amino Acids, Diamino / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D007887:Fabaceae / Q000737:chemistry; D007536:Isomerism; D017879:Isothiocyanates; D009498:Neurotoxins / Q000032:analysis* / Q000737:chemistry; D010946:Plants, Medicinal; D010947:Plants, Toxic; D013861:Thiocyanates
D000599:Amino Acids, Diamino; D017879:Isothiocyanates; D009498:Neurotoxins; D013861:Thiocyanates; C005441:phenylisothiocyanate; C100126:oxalyldiaminopropionic acid