title: C-fos expression and 2-deoxyglucose uptake in the olfactory bulb of odour-stimulated awake rats.
abstract: Using a selective monoclonal Fos antibody, we have studied the expression of the c-fos proto-oncogene in the olfactory bulb of awake rats stimulated with propionic acid vapours. We have perfected a method which allows the comparison of patterns of either cellular c-fos immunoreactivity or glomerular 2-deoxyglucose uptake in the same olfactory bulb. C-fos was expressed in local bulbar interneurones, principally granule cells. We observed that the low level of basal c-fos expression raised significantly under olfactory stimulation in the bulbar column defined by the foci of high 2-deoxyglucose glomerular uptake. This is the first demonstration that c-fos expression can be triggered by afferent olfactory input in the olfactory bulb in normally breathing awake rats. These data support the assumption that the olfactory bulb is a suitable model to elucidate the priming conditions and functional involvement of Fos protein synthesis triggered by physiological sensory stimulation.
authors.affiliation: CNRS, Université Claude Bernard/Lyon, Villeurbanne, France.
authors.forename: M
authors.initials: M
authors.lastname: Sallaz
authors.forename: F
authors.initials: F
authors.lastname: Jourdan
mesh_terms: D000818:Animals; D003847:Deoxyglucose / Q000378:metabolism*; D015870:Gene Expression / Q000187:drug effects*; D016762:Genes, fos / Q000187:drug effects*; D007150:Immunohistochemistry; D008297:Male; D009473:Neuronal Plasticity / Q000187:drug effects; D009812:Odorants; D009830:Olfactory Bulb / Q000187:drug effects / Q000378:metabolism*; D011422:Propionates / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D013268:Stimulation, Chemical
chemical_list: D011422:Propionates; D003847:Deoxyglucose; C029658:propionic acid