Estimation of protein fractional synthetic rate from tracer data.
The fractional synthetic rate (FSR) is a key parameter characterizing protein turnover that is estimated from tracer kinetic data. Formulas to estimate this parameter usually assume a precursor-product model. Assuming this model is correct, we discuss these formulas to estimate the FSR in the steady and non-steady state both for the radioactive and stable isotope tracer. Then we deal with the non-steady-state case where the FSR becomes time varying and derive formulas for its estimation. A non-steady-state case study on the flooding-dose technique for measuring protein turnover is presented.
Toffolo
G
G
Department of Electronics and Informatics, University of Padua, Italy.
Foster
D M
DM
Cobelli
C
C
D006706:Homeostasis; D007554:Isotopes; D008954:Models, Biological; D014176:Protein Biosynthesis
D007554:Isotopes