Differential expression of two genes encoding isoforms of the ATPase involved in sodium efflux in Saccharomyces cerevisiae.
The ENA2 gene encoding a P-type ATPase involved in Na+ and Li+ effluxes in Saccharomyces cerevisiae has been isolated. The putative protein encoded by ENA2 differs only in thirteen amino acids from the protein encoded by ENA1/PMR2. However, ENA2 has a very low level of expression and for this reason did not confer significant Li+ tolerance on a Li+ sensitive strain. ENA1 and ENA2 are the first two units of a tandem array of four highly homologous genes with probably homologous functions.
Garciadeblas
B
B
Departamento de Microbiología, Escuela Técnica Superior de Ingenieros Agrónomos, Madrid, Spain.
Rubio
F
F
Quintero
F J
FJ
Bañuelos
M A
MA
Haro
R
R
Rodríguez-Navarro
A
A
D000251:Adenosine Triphosphatases / Q000235:genetics*; D001483:Base Sequence; D004274:DNA, Recombinant; D004352:Drug Resistance, Microbial; D015971:Gene Expression Regulation, Enzymologic; D005800:Genes, Fungal / Q000235:genetics*; D007527:Isoenzymes / Q000235:genetics*; D007763:Lac Operon / Q000235:genetics; D008094:Lithium / Q000378:metabolism / Q000494:pharmacology; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D012964:Sodium / Q000378:metabolism*
D004274:DNA, Recombinant; D007527:Isoenzymes; D008094:Lithium; D012964:Sodium; D000251:Adenosine Triphosphatases