Lack of mutations of the p53 tumor suppressor gene in hepatocellular carcinomas induced in rats by a peroxisome proliferator.
Immunohistochemical, immunoblotting, and DNA-sequencing analyses were performed on hepatocellular carcinomas induced in rats chronically fed BR931, a peroxisome proliferator, to determine whether the tumors carried mutations or other alterations of the p53 gene. None were detected. Inactivation of this tumor suppressor gene does not appear, therefore, to be involved in the carcinogenicity of BR931, a nongenotoxic chemical hepatocarcinogen.
Smith
M L
ML
Department of Pathology, School of Medicine, University of Pittsburgh, PA 15261.
Yeleswarapu
L
L
Lombardi
B
B
Shinozuka
H
H
D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D002273:Carcinogens / Q000633:toxicity*; D004252:DNA Mutational Analysis; D004273:DNA, Neoplasm / Q000032:analysis; D016158:Genes, p53 / Q000187:drug effects*; D008114:Liver Neoplasms, Experimental / Q000139:chemically induced / Q000235:genetics*; D008297:Male; D008830:Microbodies / Q000187:drug effects; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011743:Pyrimidines / Q000633:toxicity*; D051381:Rats; D011916:Rats, Inbred F344
D002273:Carcinogens; D004273:DNA, Neoplasm; D011743:Pyrimidines; C016908:pirinixil