Physicochemical property of bovine brain 73-kDa stress protein.
1. An approximately 70-kDa protein was purified from bovine brain using an ATP-Sepharose column. 2. The protein sample was found to contain two proteins (major 73 kDa and minor 72 kDa) on two-dimensional gel electrophoresis. 3. Antibodies raised against the 73- and 72-kDa proteins cross-reacted with stress-induced HSP73 and HSP72 from HeLa cells, respectively. 4. Heparin-binding peptides were obtained from trypsin digests of HSP73.
Itoh
H
H
Department of Biochemistry, Akita University, School of Medicine, Japan.
Tashima
Y
Y
D000595:Amino Acid Sequence; D000818:Animals; D000917:Antibody Formation; D001923:Brain Chemistry; D002417:Cattle; D002845:Chromatography; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D006367:HeLa Cells; D006360:Heat-Shock Proteins / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D006493:Heparin / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D011485:Protein Binding; D011817:Rabbits
D006360:Heat-Shock Proteins; D006493:Heparin