title: The GenePrint Light method for Southern transfer and chemiluminescent detection of human genomic DNA.
abstract: A simple, fast and sensitive method to perform Southern transfer and hybridization with nonradioactive detection of genetic loci is described. With a 10-ng sample of human genomic DNA, alleles of the D2S44 locus can be detected within 7 h of completing gel electrophoresis.
authors.affiliation: R&D Department, Promega Corporation, Madison, WI 53711.
authors.forename: C J
authors.initials: CJ
authors.lastname: Sprecher
authors.forename: L R
authors.initials: LR
authors.lastname: Liss
authors.forename: J W
authors.initials: JW
authors.lastname: Schumm
mesh_terms: D015139:Blotting, Southern / Q000379:methods*; D002460:Cell Line; D004247:DNA / Q000032:analysis*; D015252:Deoxyribonucleases, Type II Site-Specific; D006801:Humans; D008163:Luminescent Measurements; D009691:Nucleic Acid Denaturation; D009693:Nucleic Acid Hybridization / Q000379:methods; D014466:Ultraviolet Rays
chemical_list: D004247:DNA; D015252:Deoxyribonucleases, Type II Site-Specific; C024403:GGCC-specific type II deoxyribonucleases