Crystallization and preliminary X-ray crystallographic analysis of arylesterase from Pseudomonas fluorescens.
Large crystals of arylesterase from Pseudomonas fluorescens have been grown at room temperature using ammonium sulfate as a precipitant. They grow to dimensions of 0.7 x 0.7 x 0.6 mm3 within a month. The crystals belong to the trigonal space group P3(1) (or P3(2)), with unit cell dimensions of a = 147.12 A and c = 131.08 A. The asymmetric unit seems to contain six molecules of dimeric arylesterase, with corresponding crystal volume per protein mass (VM) of 2.53 A3/Da and solvent fraction of 51.5% by volume. The crystals diffract to at least 2.2 A Bragg spacing when exposed to X-rays from a rotating-anode source. X-ray data have been collected to 2.9 A Bragg spacing from native crystals.
Kim
K K
KK
Department of Chemistry, College of Natural Sciences, Seoul National University, Korea.
Hwang
K Y
KY
Choi
K D
KD
Kang
J H
JH
Yoo
O J
OJ
Suh
S W
SW
D002265:Carboxylic Ester Hydrolases / Q000737:chemistry*; D003460:Crystallization; D011551:Pseudomonas fluorescens / Q000201:enzymology*; D014961:X-Ray Diffraction
D002265:Carboxylic Ester Hydrolases; C019734:arylesterase