Xylitol-induced increase in purine degradation: a role of erythrocytes.
We administered xylitol intravenously to normal subjects to investigate the mechanism of xylitol-induced increase in the purine degradation in humans. Xylitol increased the plasma concentrations of hypoxanthine, xanthine and uric acid but decreased the blood concentration of pyruvic acid. The erythrocyte concentrations of IMP, AMP, ADP, glyceraldehyde 3-phosphate and fructose 1,6-diphosphate as well as the urinary excretion of hypoxanthine and xanthine were increased, while the erythrocyte concentration of ATP was decreased. In addition, the in vitro incubation studies using erythrocytes demonstrated that both xylitol-induced purine degradation and xylitol-induced inhibition of the conversion of glyceraldehyde 3-phosphate to 1,3-bisphosphoglycerate were protected by pyruvic acid. These results indicate that xylitol-induced impairment of glycolysis in erythrocytes contributes to the observed xylitol-induced increase in the purine degradation in the body.
Yamamoto
T
T
Third Department of Internal Medicine, Hyogo College of Medicine, Japan.
Moriwaki
Y
Y
Suda
M
M
Agbedana
E O
EO
Takahashi
S
S
Nasako
Y
Y
Higashino
K
K
Nakamo
T
T
Hiroishi
K
K
D000328:Adult; D004912:Erythrocytes / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D006019:Glycolysis / Q000187:drug effects; D006801:Humans; D007042:Hypoxanthines / Q000097:blood; D007275:Injections, Intravenous; D008297:Male; D011687:Purines / Q000097:blood* / Q000652:urine; D011773:Pyruvates / Q000097:blood; D012709:Serum Albumin / Q000187:drug effects / Q000378:metabolism; D014993:Xylitol / Q000008:administration & dosage / Q000494:pharmacology*
D007042:Hypoxanthines; D011687:Purines; D011773:Pyruvates; D012709:Serum Albumin; D014993:Xylitol