title: Lambda kil-mediated lysis requires the phage context.
abstract: The lambda kil gene has been shown to be responsible for premature lysis effected by addition of chloramphenicol between 15 and 20 min after thermal induction of a lambda prophage. Here, we localized the kil reading frame. The kil gene, represented by lambda orf47, overlaps genes cIII and gam. Expression of the plasmid-borne kil gene resulted in growth arrest, a reduction of colony-forming units and filament formation. However, kil-mediated cell lysis could not be triggered by chloramphenicol when the plasmid borne kil gene was expressed, suggesting that kil-induced cell lysis requires the phage context.
authors.affiliation: Institute of Microbiology and Genetics, University of Vienna, Austria.
authors.forename: G R
authors.initials: GR
authors.lastname: Reisinger
authors.forename: A
authors.initials: A
authors.lastname: Rietsch
authors.forename: W
authors.initials: W
authors.lastname: Lubitz
authors.forename: U
authors.initials: U
authors.lastname: Bläsi
mesh_terms: D010582:Bacteriophage lambda / Q000235:genetics* / Q000502:physiology; D015500:Chloramphenicol O-Acetyltransferase / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development; D005814:Genes, Viral; D007700:Kinetics; D016366:Open Reading Frames; D010957:Plasmids; D015183:Restriction Mapping; D013997:Time Factors
chemical_list: D015500:Chloramphenicol O-Acetyltransferase