Cholesterol feeding induces hypertriglyceridaemia in hamsters and increases the activity of the Mg(2+)-dependent phosphatidate phosphohydrolase in the liver.
(1) Feeding increased cholesterol to hamsters resulted in a dose-dependent increase in cholesterol and triacylglycerol in very-low-density lipoprotein (VLDL), in serum non-esterified fatty acids and in the activity of the Mg(2+)-dependent phosphatidate phosphohydrolase in liver. (2) The effects of increasing dietary cholesterol by 0.12% (w/w) in addition to feeding fat (14%, w/w) were dependent upon the nature of the fat. Lard in the presence of 0.12% (w/w) cholesterol increased serum triacylglycerols as did olive oil. By contrast, sunflower oil did not cause a significant change in serum triacylglycerol concentrations. (3) There was a highly positive correlation between VLDL triacylglycerol and VLDL cholesterol concentrations suggesting that, at least in this model, there is a close relationship between hypertriglyceridaemia and hypercholesterolaemia.
Sessions
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VA
Department of Applied Biochemistry and Food Science, University of Nottingham, Faculty of Agricultural and Food Sciences, Sutton Bonington, Loughborough, UK.
Martin
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A
Gomez-Muñoz
A
A
Brindley
D N
DN
Salter
A M
AM
D000818:Animals; D017304:Annexin A5 / Q000378:metabolism; D002791:Cholesterol, Dietary; D006224:Cricetinae; D004041:Dietary Fats / Q000008:administration & dosage* / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D015228:Hypertriglyceridemia / Q000139:chemically induced* / Q000201:enzymology; D008079:Lipoproteins, VLDL / Q000378:metabolism; D008099:Liver / Q000201:enzymology* / Q000378:metabolism; D008274:Magnesium / Q000378:metabolism*; D008297:Male; D008647:Mesocricetus; D008954:Models, Biological; D010711:Phosphatidate Phosphatase / Q000378:metabolism*; D013045:Species Specificity; D014280:Triglycerides / Q000097:blood / Q000378:metabolism
D017304:Annexin A5; D002791:Cholesterol, Dietary; D004041:Dietary Fats; D005227:Fatty Acids; D008079:Lipoproteins, VLDL; D014280:Triglycerides; D010711:Phosphatidate Phosphatase; D008274:Magnesium