title: High-performance liquid chromatography of human glycoprotein hormones.
abstract: The chromatographic behavior of the glycoprotein hormones from human pituitary glands and of placental origin [thyroid-stimulating hormone, luteinizing hormone and chorionic gonadotropin (CG)] was studied. It was shown that hydrophobic interaction chromatography on a microparticulate packing and anion-exchange HPLC can be applied for the purification of these hormones. Reversed-phase HPLC on wide-pore C4-bonded silica at neutral pH can be applied for the determination of the above hormones and for the isolation of pure CG and its subunits.
authors.affiliation: NPO "Biotechnologia", Moscow, Russian Federation.
authors.forename: M A
authors.initials: MA
authors.lastname: Chlenov
authors.forename: E I
authors.initials: EI
authors.lastname: Kandyba
authors.forename: L V
authors.initials: LV
authors.lastname: Nagornaya
authors.forename: I L
authors.initials: IL
authors.lastname: Orlova
authors.forename: Y V
authors.initials: YV
authors.lastname: Volgin
mesh_terms: D006063:Chorionic Gonadotropin / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002852:Chromatography, Ion Exchange; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007986:Luteinizing Hormone / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet; D013972:Thyrotropin / Q000032:analysis*
chemical_list: D006063:Chorionic Gonadotropin; D007986:Luteinizing Hormone; D013972:Thyrotropin