Abnormal peptidoglycan produced in a methicillin-resistant strain of Staphylococcus aureus grown in the presence of methicillin: functional role for penicillin-binding protein 2A in cell wall synthesis.
Upon the addition of methicillin even at relatively low concentrations (5 mug/ml or 0.3% of the MIC) to the medium, methicillin-resistant staphylococci shift to the production of a new peptidoglycan with an abnormal muropeptide composition which may be the synthetic product of penicillin-binding protein 2A.
de Jonge
B L
BL
Rockefeller University, New York, New York 10021.
Tomasz
A
A
D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D002352:Carrier Proteins / Q000502:physiology*; D002473:Cell Wall / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D002851:Chromatography, High Pressure Liquid; D006602:Hexosyltransferases; D008712:Methicillin / Q000494:pharmacology*; D016106:Methicillin Resistance / Q000502:physiology*; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D002267:Muramoylpentapeptide Carboxypeptidase / Q000502:physiology*; D046915:Penicillin-Binding Proteins; D010457:Peptidoglycan / Q000032:analysis / Q000096:biosynthesis*; D010458:Peptidyl Transferases; D013211:Staphylococcus aureus / Q000378:metabolism* / Q000648:ultrastructure; D013379:Substrate Specificity
D001426:Bacterial Proteins; D002352:Carrier Proteins; D046915:Penicillin-Binding Proteins; D010457:Peptidoglycan; D010458:Peptidyl Transferases; D006602:Hexosyltransferases; D002267:Muramoylpentapeptide Carboxypeptidase; D008712:Methicillin