Presence of lipocortins I and IV, but not II and VI, in human platelets.
The present investigation revealed the presence of lipocortins I and IV, but not lipocortins II and VI, in human platelets. Lipocortin I was found in the Triton-soluble fraction of both resting and thrombin-activated platelets and was not covalently bound to skeletal components. Without detergents, when resting platelets were lysed and fractionated in the absence of Ca2+, lipocortin I was found only in the cytosolic fraction, whereas, in the presence of Ca2+, lipocortin I was associated only with the crude particulate and not with the membrane nor the cytosolic fractions.
Eldering
J A
JA
Department of Medicine, Inselspital, Berne, Switzerland.
Kocher
M
M
Clemetson
J M
JM
Clemetson
K J
KJ
Frey
F J
FJ
Frey
B M
BM
D017305:Annexin A1 / Q000097:blood*; D017306:Annexin A2 / Q000097:blood; D017314:Annexin A4 / Q000097:blood*; D017317:Annexin A6 / Q000097:blood; D001792:Blood Platelets / Q000737:chemistry* / Q000187:drug effects; D015153:Blotting, Western; D002118:Calcium / Q000494:pharmacology; D003594:Cytoplasmic Granules / Q000737:chemistry; D003599:Cytoskeleton / Q000737:chemistry; D003600:Cytosol / Q000737:chemistry; D003902:Detergents / Q000494:pharmacology; D004533:Egtazic Acid / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D015539:Platelet Activation; D013917:Thrombin / Q000494:pharmacology
D017305:Annexin A1; D017306:Annexin A2; D017314:Annexin A4; D017317:Annexin A6; D003902:Detergents; D004533:Egtazic Acid; D013917:Thrombin; D002118:Calcium