Intertypic differentiation and detection of intratypic variants among canine and phocid morbillivirus isolates by kinetic neutralization using a novel immunoplaque assay.
Intertypic antigenic differences and the intratypic variability of the closely related canine (CVD) and phocid distemper viruses (PDV) were examined using a molecular (monoclonal antibodies specific for the H- and F-glycoproteins) and a functional (kinetic neutralization, KN) approach. KN studies were carried out using a novel immunoplaque technique which combined conventional plaque assay and antigen-specific enzyme-immunostaining techniques. Morbillivirus isolates of canine and phocid origin clearly formed two separate groups. Minor antigenic differences were also evident within each cluster. A distemper isolate of mustelid origin was distinguishable from both CDV- and PDV-like prototype viruses by kinetic neutralization.
Harder
T C
TC
Institute of Virology, Hannover Veterinary School, Germany.
Klusmeyer
K
K
Frey
H R
HR
Orvell
C
C
Liess
B
B
D000818:Animals; D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D004217:Distemper Virus, Canine / Q000276:immunology* / Q000302:isolation & purification; D004285:Dogs; D005069:Evaluation Studies as Topic; D008459:Measles virus / Q000276:immunology / Q000302:isolation & purification; D009500:Neutralization Tests / Q000379:methods; D010252:Paramyxoviridae / Q000145:classification / Q000276:immunology* / Q000302:isolation & purification; D046048:Seals, Earless / Q000382:microbiology; D010948:Viral Plaque Assay / Q000379:methods*; D014773:Virology / Q000379:methods*
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral