An autosampler for fermentation.
The operation of an autosampler, constructed from a peristatic pump, an interval timer and a fraction collector, for the removal of whole broth fermentation samples is described. The autosampler repetitively removes samples of user-determined size at repetitive time intervals. The sampler is intended for use with low containment fermentations. A comparison of the autosampler and the manual sampler of a fermenter showed good correlation of substrate consumption rates when changes were sizeable and superior results were obtained with the autosampler when the changes were subtle during a typical diauxic fermentation of Azotobacter vinelandii.
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W J
WJ
Department of Microbiology, University of Alberta, Edmonton, Canada.
Rudy
B
B
D000085:Acetates / Q000378:metabolism; D000641:Ammonia / Q000378:metabolism; D001322:Autoanalysis / Q000295:instrumentation; D016948:Azotobacter vinelandii / Q000254:growth & development / Q000378:metabolism*; D001431:Bacteriological Techniques / Q000295:instrumentation*; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D006863:Hydrogen-Ion Concentration
D000085:Acetates; D000641:Ammonia; D005947:Glucose