Mutagenesis of the virion-sense open reading frames of tomato leaf curl geminivirus.
A series of frame shift, deletion, and inversion mutants in the virion-sense open reading frames (ORFs) of the monopartite geminivirus tomato leaf curl virus have been constructed and their ability to replicate, produce single-stranded DNA, spread, and cause symptoms in tomato plants has been investigated. Disruptions in the V1 ORF lead to symptomless, systemic infections with a reduced titer of all viral DNA forms while interruptions in the V2 (coat protein) ORF disrupted spread of the virus. Mutagenesis of the virion-sense ORFs did not affect the replication of viral double-stranded DNA, although both V1 and V2 products appear to play a role in the accumulation of viral single-stranded DNA.
Rigden
J E
JE
CSIRO Division of Horticulture, Adelaide, Australia.
Dry
I B
IB
Mullineaux
P M
PM
Rezaian
M A
MA
D015139:Blotting, Southern; D007446:Chromosome Inversion; D004277:DNA, Single-Stranded / Q000096:biosynthesis; D004279:DNA, Viral / Q000096:biosynthesis; D003673:Defective Viruses / Q000235:genetics / Q000502:physiology; D016368:Frameshift Mutation; D016296:Mutagenesis; D016366:Open Reading Frames; D010942:Plant Viruses / Q000235:genetics* / Q000502:physiology; D015183:Restriction Mapping; D017384:Sequence Deletion; D014771:Virion / Q000235:genetics* / Q000502:physiology; D014779:Virus Replication / Q000235:genetics*
D004277:DNA, Single-Stranded; D004279:DNA, Viral