Efficient random mutagenesis method with adjustable mutation frequency by use of PCR and dITP.
Spee
J H
JH
Department of Biophysical Chemistry, Netherlands Institute for Dairy Research (NIZO), Ede.
de Vos
W M
WM
Kuipers
O P
OP
D015544:Inositol 1,4,5-Trisphosphate / Q000031:analogs & derivatives*; D016296:Mutagenesis; D016133:Polymerase Chain Reaction / Q000379:methods*
C067150:6-deoxyinositol 1,4,5-triphosphate; D015544:Inositol 1,4,5-Trisphosphate