Expression of dopamine D1A receptor gene in proximal tubule of rat kidneys.
The existence of dopamine receptor subtypes outside the central nervous system has been suggested by pharmacological and biochemical techniques. Whether the renal dopamine receptors are distinct from those cloned from the brain remains to be determined. Indeed, the expression of any of these receptor genes in the kidney has not been demonstrated definitively. In this study, we amplified D1A receptor cDNA from microdissected proximal convoluted tubules of the rat kidney by reverse transcription-polymerase chain reaction; primers were based on the sequence of rat D1A cDNA cloned from the brain and corresponded to the third cytoplasmic loop of the receptor. Specificity of the amplified products was verified by restriction analysis, Southern blots, and sequencing. Furthermore, solution hybridization indicated the presence of a single ribonuclease-protected RNA species corresponding to the D1A receptor mRNA in proximal tubules. These studies report for the first time the presence of D1A receptor message in the kidney.
Yamaguchi
I
I
Department of Pathology, University of Virginia Medical Center, Charlottesville 22908.
Jose
P A
PA
Mouradian
M M
MM
Canessa
L M
LM
Monsma
F J
FJ
Sibley
D R
DR
Takeyasu
K
K
Felder
R A
RA
D000818:Animals; D001483:Base Sequence; D015870:Gene Expression; D007687:Kidney Tubules, Proximal / Q000502:physiology*; D015335:Molecular Probes / Q000235:genetics; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D051381:Rats; D011921:Rats, Inbred WKY; D017447:Receptors, Dopamine D1 / Q000235:genetics*; D012260:Ribonucleases
D015335:Molecular Probes; D017447:Receptors, Dopamine D1; D012260:Ribonucleases