The vanB gene of vancomycin-resistant Enterococcus faecalis V583 is structurally related to genes encoding D-Ala:D-Ala ligases and glycopeptide-resistance proteins VanA and VanC.
We report the cloning and sequencing of a 632-bp amplified fragment internal to the vanB gene of vancomycin-resistant (VmR) Enterococcus (En.) faecalis V583. The DNA fragment hybridized to VmR strains of En. faecium and En. faecalis, but not to their susceptible derivatives.
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Unité des Agents Antibactériens, Institut Pasteur, Paris, France.
Sahm
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Courvalin
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D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D019729:Carbon-Oxygen Ligases; D004352:Drug Resistance, Microbial / Q000235:genetics*; D013293:Enterococcus faecalis / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D005798:Genes, Bacterial; D006020:Glycopeptides / Q000494:pharmacology; D008969:Molecular Sequence Data; D010453:Peptide Synthases / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D014640:Vancomycin / Q000494:pharmacology*
D001426:Bacterial Proteins; D006020:Glycopeptides; C071437:VanA ligase, Bacteria; C079913:VanB protein, Enterococcus; C079914:VanC protein, Bacteria; D014640:Vancomycin; D019729:Carbon-Oxygen Ligases; D010453:Peptide Synthases; C019638:D-alanylalanine synthetase