title: An Alu sequence interrupts a human 5-hydroxytryptamine1D receptor pseudogene.
abstract: Molecular cloning studies have now identified six HTR genes encoding the biosynthesis of the structurally homologous human serotonin (5-hydroxytryptamine; 5-HT) receptors, namely 5-HTR1A, 5-HTR1B, 5-HTR1C, 5-HTR1D, 5-HTR2 and 5-HTRS31. Several of these receptors are encoded by intronless genes, and we now report the cloning of another intronless serotonergic HTR gene. This gene was cloned by a method using the polymerase chain reaction. The nucleotide sequence of this gene is most closely homologous to the 5-HTR1D gene; however, several stop codons, frame shifts and deletions are present in the coding region suggesting that this is a pseudogene which could not encode a functional receptor. Sequence analysis also revealed that the coding sequence of this pseudogene is disrupted by insertion of a 283-bp Alu repeat sequence.
authors.affiliation: Addiction Research Foundation, Toronto, Ontario, Canada.
authors.forename: T
authors.initials: T
authors.lastname: Nguyen
authors.forename: A
authors.initials: A
authors.lastname: Marchese
authors.forename: J L
authors.initials: JL
authors.lastname: Kennedy
authors.forename: A
authors.initials: A
authors.lastname: Petronis
authors.forename: S J
authors.initials: SJ
authors.lastname: Peroutka
authors.forename: P H
authors.initials: PH
authors.lastname: Wu
authors.forename: B F
authors.initials: BF
authors.lastname: O'Dowd
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D003001:Cloning, Molecular; D006801:Humans; D008969:Molecular Sequence Data; D002679:Pan troglodytes; D016133:Polymerase Chain Reaction; D011544:Pseudogenes; D051381:Rats; D011985:Receptors, Serotonin / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
chemical_list: D011985:Receptors, Serotonin