title: Phosphorylation of an acid-soluble nuclear protein in response to catecholamine in cultured rat cardiocytes.
abstract: Effect of norepinephrine on the phosphorylation of nuclear proteins was examined in cultured rat cardiocytes. Differential effects of phorbol esters, endothelin and platelet activating factor on the phosphorylation of a nuclear acid soluble fraction were evaluated. A specific phosphorylated nuclear acid soluble protein was identified in response to norepinephrine. The increase in phosphorylation in the norepinephrine-treated cardiocytes is significantly greater than 8 fold (p < 0.001) as compared to the control group. This nuclear acid soluble protein has a high content of serine (> 12%) as well as acidic amino acids (> 20%) which is distinctively different from the histones as analyzed by amino acid composition. The specific phosphorylation of this nuclear protein (Mr 31 Kda) can be induced by norepinephrine within 5 minutes, indicating of an early gene activation event which modulates nucleosomal structure by phosphorylation.
authors.affiliation: Department of Medicine, National Taiwan University, R.O.C.
authors.forename: J J
authors.initials: JJ
authors.lastname: Chen
authors.forename: C C
authors.initials: CC
authors.lastname: Liew
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002478:Cells, Cultured; D005786:Gene Expression Regulation / Q000187:drug effects; D066298:In Vitro Techniques; D009218:Myosins / Q000235:genetics; D009638:Norepinephrine / Q000494:pharmacology*; D009687:Nuclear Proteins / Q000737:chemistry / Q000378:metabolism*; D010750:Phosphoproteins / Q000737:chemistry / Q000378:metabolism*; D010766:Phosphorylation; D010972:Platelet Activating Factor / Q000494:pharmacology; D051381:Rats; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013997:Time Factors; D014157:Transcription Factors / Q000378:metabolism; D015533:Transcriptional Activation
chemical_list: D000596:Amino Acids; D009687:Nuclear Proteins; D010750:Phosphoproteins; D010972:Platelet Activating Factor; D014157:Transcription Factors; D009218:Myosins; D013755:Tetradecanoylphorbol Acetate; D009638:Norepinephrine