Reduction of fatty acid hydroperoxides by human parotid saliva.
Arachidonic acid hydroperoxide (15-hydroperoxyeicosatetraenoic acid; 15-HPETE) was introduced into human parotid saliva and incubated at 37 degrees C. Straight phase high-performance liquid chromatography analysis of the reaction mixture showed that 15-HPETE was detoxified to its reduced form, 15-hydroxyeicosatetraenoic acid, in the presence of glutathione. Therefore, it is concluded that human parotid saliva possesses fatty acid hydroperoxide-reducing ability. However, its effectiveness was found to be lower than that of blood plasma.
Terao
J
J
National Food Research Institute, Ministry of Agriculture, Forestry and Fisheries, Ibaraki, Japan.
Nagao
A
A
Yuki
H
H
Itoh
Y
Y
D000328:Adult; D002851:Chromatography, High Pressure Liquid; D005978:Glutathione / Q000494:pharmacology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006893:Hydroxyeicosatetraenoic Acids / Q000378:metabolism; D015289:Leukotrienes / Q000378:metabolism*; D008054:Lipid Peroxides / Q000378:metabolism*; D010084:Oxidation-Reduction; D010306:Parotid Gland / Q000378:metabolism*; D012463:Saliva / Q000378:metabolism*
D006893:Hydroxyeicosatetraenoic Acids; D015289:Leukotrienes; D008054:Lipid Peroxides; C025086:15-hydroperoxy-5,8,11,13-eicosatetraenoic acid; C025984:15-hydroxy-5,8,11,13-eicosatetraenoic acid; D005978:Glutathione