title: Evaluation of an immunoblot assay for serological confirmation and differentiation of human T-cell lymphotropic virus types I and II.
abstract: The confirmation of infection with human T-cell lymphotropic virus type I (HTLV-I) and type II (HTLV-II) currently involves multiple assays. These include Western blot (immunoblot) (WB) and/or radioimmunoprecipitation assay for detection of antibodies to HTLV-specific viral proteins and polymerase chain reaction and/or peptide-based enzyme immunoassays for differentiating between the two viruses. We undertook an evaluation of a modified WB assay that includes native HTLV-I viral proteins from MT-2 cells spiked with an HTLV-I transmembrane glycoprotein (recombinant p21e) and the HTLV-I- and HTLV-II-specific recombinant proteins MTA-1 and K55. The test panel consisted of well-characterized sera from U.S. blood donors, American Indians, intravenous drug users, and patients seen in sexually transmitted disease clinics. Of 158 HTLV-I/II-seropositive serum specimens tested, 156 (98.7%) were confirmed and typed as HTLV-I or HTLV-II. Of 82 HTLV-I/II-seroindeterminate or -seronegative serum specimens, only 1 was classified as HTLV-II positive: the sample had weak gag p19 and strong gag p24 and env p21e reactivity and was radioimmunoprecipitation assay negative for env gp61/68 but polymerase chain reaction positive for HTLV-II. The specificity of the modified WB for confirming and typing serum samples was therefore 100%. We conclude that this WB assay is useful for confirming and typing HTLV infection and can help simplify HTLV-I/II testing algorithms.
authors.affiliation: Division of Viral and Rickettsial Diseases, Centers for Disease Control, Atlanta, Georgia 30333.
authors.forename: B D
authors.initials: BD
authors.lastname: Roberts
authors.forename: S K
authors.initials: SK
authors.lastname: Foung
authors.forename: J J
authors.initials: JJ
authors.lastname: Lipka
authors.forename: J E
authors.initials: JE
authors.lastname: Kaplan
authors.forename: K G
authors.initials: KG
authors.lastname: Hadlock
authors.forename: G R
authors.initials: GR
authors.lastname: Reyes
authors.forename: L
authors.initials: L
authors.lastname: Chan
authors.forename: W
authors.initials: W
authors.lastname: Heneine
authors.forename: R F
authors.initials: RF
authors.lastname: Khabbaz
mesh_terms: D015153:Blotting, Western / Q000379:methods* / Q000706:statistics & numerical data; D005069:Evaluation Studies as Topic; D015481:HTLV-I Antibodies / Q000097:blood; D015490:HTLV-I Infections / Q000175:diagnosis; D015482:HTLV-II Antibodies / Q000097:blood; D015491:HTLV-II Infections / Q000175:diagnosis; D015368:Human T-lymphotropic virus 1 / Q000145:classification / Q000276:immunology* / Q000302:isolation & purification; D015367:Human T-lymphotropic virus 2 / Q000145:classification / Q000276:immunology* / Q000302:isolation & purification; D006801:Humans; D012680:Sensitivity and Specificity; D012703:Serotyping
chemical_list: D015481:HTLV-I Antibodies; D015482:HTLV-II Antibodies