Bisbenzylisoquinoline alkaloids inhibit tumor promotion by 12-O-tetradecanoylphorbol-13-acetate in two-stage carcinogenesis in mouse skin.
Bisbenzylisoquinoline alkaloids, cepharanthine, berbamine and isotetrandrine, were isolated from Stephania cepharantha Hayata. These compounds inhibit arachidonic acid-induced inflammation in mice. We have found that cepharanthine inhibits tumor promotion after topical application and oral administration in two-stage carcinogenesis in mouse skin. Furthermore, topical application of berbamine (2 mumol/mouse) and isotetrandrine (2 mumol/mouse) markedly suppressed the tumor-promoting effect of 12-O-tetradecanoylphorbol-13-acetate (1 microgram) in mouse skin initiated with 7,12-dimethylbenz[a]anthracene (50 micrograms), at a grade corresponding to that of cepharanthine.
Yasukawa
K
K
College of Pharmacy, Nihon University, Chiba, Japan.
Akasu
M
M
Takeuchi
M
M
Takido
M
M
D015127:9,10-Dimethyl-1,2-benzanthracene; D000287:Administration, Topical; D000470:Alkaloids / Q000627:therapeutic use*; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000627:therapeutic use*; D016588:Anticarcinogenic Agents / Q000627:therapeutic use*; D000970:Antineoplastic Agents / Q000627:therapeutic use; D016718:Arachidonic Acid; D044182:Benzylisoquinolines; D005260:Female; D007249:Inflammation / Q000139:chemically induced / Q000517:prevention & control; D007546:Isoquinolines / Q000627:therapeutic use*; D051379:Mice; D008813:Mice, Inbred ICR; D012878:Skin Neoplasms / Q000139:chemically induced / Q000517:prevention & control*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D052117:Benzodioxoles
D015127:9,10-Dimethyl-1,2-benzanthracene; D000470:Alkaloids; D000894:Anti-Inflammatory Agents, Non-Steroidal; D016588:Anticarcinogenic Agents; D000970:Antineoplastic Agents; D016718:Arachidonic Acid; D044182:Benzylisoquinolines; D007546:Isoquinolines; D013755:Tetradecanoylphorbol Acetate; C009438:tetrandrine; C006947:cepharanthine; D052117:Benzodioxoles; C027870:berbamine