Syrian hamster embryo cell lines useful for detecting transforming genes in mouse tumours: detection of transforming genes in X-ray-related mouse tumours.
The Syrian hamster embryo cell lines, SHOK and MC-1, were used as recipient cells for DNA transfection assay to detect transforming genes in experimental mouse tumours. A mouse repeat sequence was utilised to check whether each transformed focus included mouse genomic DNA in the Hamster background. We investigated five mouse tumours that are related to X-ray radiation, and detected activated c-K-ras, c-mos, and c-cot oncogenes which induced foci of hamster cells. These results show that SHOK and MC-1 cells have unique properties for detecting transforming genes in experimental mouse tumours.
Sasai
H
H
Department of Oncogene Research, Osaka University, Japan.
Higashi
T
T
Nakamori
S
S
Miyoshi
J
J
Suzuki
F
F
Nomura
T
T
Kakunaga
T
T
D016475:3T3 Cells / Q000502:physiology; D000818:Animals; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D006224:Cricetinae; D004273:DNA, Neoplasm / Q000235:genetics; D004622:Embryo, Mammalian; D005347:Fibroblasts / Q000502:physiology; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D008647:Mesocricetus; D051379:Mice; D009374:Neoplasms, Experimental / Q000235:genetics*; D009381:Neoplasms, Radiation-Induced / Q000235:genetics*; D009857:Oncogenes / Q000235:genetics*; D014162:Transfection
D004273:DNA, Neoplasm