The effect of basic fibroblast growth factor on the growth and function of human thyrocytes.
Basic fibroblast growth factor (bFGF) was quantitated in human primary thyrocyte cultures and thyroid cell lines produced by transfection with pSV3neo. Immunoreactive-bFGF (ir-bFGF) bound to heparin-Sepharose affinity columns eluted with 1.8-2.0 mol NaCl/l and had a molecular weight of approximately 17,000. Recombinant human bFGF in the presence of 5% serum increased the growth of transfected human thyrocytes but not the growth of primary human thyrocytes. Preincubation of cells with up to 100 micrograms bFGF/l potentiated TSH-stimulated cAMP release from the transfected cells but inhibited release from primary human thyroid cultures. bFGF may be an important modulator of thyroid cell function and growth.
Taylor
A H
AH
Department of Cellular and Molecular Sciences, St George's Hospital Medical School, London, U.K.
Millatt
L J
LJ
Whitley
G S
GS
Johnstone
A P
AP
Nussey
S S
SS
D002478:Cells, Cultured; D002846:Chromatography, Affinity; D016222:Fibroblast Growth Factor 2 / Q000032:analysis / Q000378:metabolism*; D006801:Humans; D011863:Radioimmunoassay; D013961:Thyroid Gland / Q000737:chemistry / Q000166:cytology / Q000254:growth & development / Q000502:physiology*
D016222:Fibroblast Growth Factor 2