title: Regulatory relevance of lipoproteins in interleukin-2 stimulated lymphocytes in vitro.
abstract: The regulatory role of very low density lipoproteins (VLDL) in de novo protein synthesis and in natural killer (NK) mediated cytotoxicity against cancer cells was evaluated in IL-2 activated peripheral blood mononuclear cells (PBL). Supplementing of VLDL to the incubation medium induced a potent dose-related inhibitory effect on the synthesis and secretion of (35S) labelled proteins. The maximal inhibitory response was observed at 50 micrograms/ml cholesterol VLDL. The effect of VLDL on the IL-2 induced ability of lymphocytes to kill cancer cells and to interfere with target cell proliferation was then evaluated. Maximal cytotoxicity was observed at 50 micrograms VLDL/ml as cholesterol. This VLDL-dependent stimulatory effect was associated with a significant decrease in the proliferative effect of the medium conditioned by PBL. The possibility that VLDL mediate the regulation of immune functions by interacting with metabolic patterns expressed by IL-2 stimulated lymphocytes is discussed in light of these results.
authors.affiliation: Institute of Endocrinology, University of Milan, Italy.
authors.forename: T
authors.initials: T
authors.lastname: Brevini
authors.forename: M
authors.initials: M
authors.lastname: Mantovani
authors.forename: A
authors.initials: A
authors.lastname: Corsini
authors.forename: A
authors.initials: A
authors.lastname: Gambetta
authors.forename: F
authors.initials: F
authors.lastname: Gandolfi
authors.forename: F
authors.initials: F
authors.lastname: Bozzetti
authors.forename: E
authors.initials: E
authors.lastname: Agradi
mesh_terms: D000328:Adult; D002478:Cells, Cultured; D003602:Cytotoxicity, Immunologic / Q000187:drug effects / Q000276:immunology; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology*; D007694:Killer Cells, Natural / Q000187:drug effects / Q000276:immunology; D008079:Lipoproteins, VLDL / Q000502:physiology*; D008214:Lymphocytes / Q000187:drug effects* / Q000276:immunology; D014176:Protein Biosynthesis; D011506:Proteins / Q000378:metabolism; D014407:Tumor Cells, Cultured
chemical_list: D007376:Interleukin-2; D008079:Lipoproteins, VLDL; D011506:Proteins