title: Peripheral T-cell lymphoma with unique immunologic features.
abstract: BACKGROUND
  The autologous mixed lymphocyte reaction (AMLR) is an important immunoregulatory phenomenon in human immune disorders. The authors have determined the phenotype and assessed the response of malignant lymph node T-cells, from histologically and immunologically proven cases of peripheral T-cell lymphoma, in AMLR and allogeneic mixed lymphocyte reaction (MLR) and studied the secretion of lymphokines.
  
  
  METHODS
  The proliferative response, tritiated 3H-thymidine incorporation assay, was used to determine the AMLR and allogeneic MLR of the responding T-cells. An interleukin-2 (IL-2)-dependent T-cell line (CTLL) was used for the production of IL-2 by phytohemagglutinin-stimulated T-cells in a cytotoxic assay. B-cell growth and differentiation factor activity of T-cells was studied by enzyme-linked immunosorbent assay.
  
  
  RESULTS
  The AMLR of malignant lymph node T-cells was increased characteristically in 12 of the 14 lymphoma cases studied; however, that of the blood T-cells was decreased. The allogeneic MLR of the malignant lymph node T-cells and blood-purified T-cells of the eight cases investigated was decreased. Expression or deficiency of CD2 and CD3 antigens on malignant T-cells did not show any difference in the AMLR assay.
  
  
  CONCLUSIONS
  This study demonstrates an important tendency of malignant T-cells from patients with peripheral T-cell lymphoma to proliferate in AMLR. The highly augmented AMLR but deficient allogeneic MLR observed in these malignant T-cells indicate that autologous recognitive events may play an important role in the immunopathogenesis of this human disease.
authors.affiliation: Department of Clinical and Laboratory Hematology, King Saud University Medical College, Abha, Saudi Arabia.
authors.forename: A
authors.initials: A
authors.lastname: Sheikha
authors.forename: M
authors.initials: M
authors.lastname: al-Janadi
authors.forename: A
authors.initials: A
authors.lastname: al-Saigh
authors.forename: T
authors.initials: T
authors.lastname: Malatani
authors.forename: S
authors.initials: S
authors.lastname: Raziuddin
mesh_terms: D000328:Adult; D000368:Aged; D001706:Biopsy; D003208:Concanavalin A / Q000494:pharmacology; D005260:Female; D006801:Humans; D007376:Interleukin-2 / Q000502:physiology; D008198:Lymph Nodes / Q000276:immunology / Q000473:pathology; D008213:Lymphocyte Activation / Q000187:drug effects / Q000276:immunology; D007959:Lymphocyte Culture Test, Mixed; D016411:Lymphoma, T-Cell, Peripheral / Q000097:blood / Q000276:immunology* / Q000503:physiopathology; D008297:Male; D008875:Middle Aged; D010835:Phytohemagglutinins / Q000494:pharmacology; D013601:T-Lymphocytes / Q000276:immunology / Q000502:physiology*; D014376:Tuberculosis / Q000097:blood / Q000276:immunology / Q000473:pathology
chemical_list: D007376:Interleukin-2; D010835:Phytohemagglutinins; D003208:Concanavalin A