Crystallization and preliminary X-ray analysis of the periplasmic fragment of CyoA-a subunit of the Escherichia coli cytochrome o complex.
CyoA, an integral membrane protein, is a subunit of the Escherichia coli cytochrome o quinol oxidase complex. The C-terminal periplasmic domain of CyoA has been expressed in E. coli, purified and crystallized. Crystals were grown using ammonium sulphate as a precipitant. They have space group I222 or I2(1)2(1)2(1) and diffract X-rays to 2.3 A resolution.
van der Oost
J
J
European Molecular Biology Laboratory, Heidelberg, Germany.
Musacchio
A
A
Pauptit
R A
RA
Ceska
T A
TA
Wierenga
R K
RK
Saraste
M
M
D003460:Crystallization; D003573:Cytochrome b Group; D003580:Cytochromes / Q000737:chemistry*; D004926:Escherichia coli / Q000201:enzymology*; D029968:Escherichia coli Proteins; D014961:X-Ray Diffraction
D003573:Cytochrome b Group; D003580:Cytochromes; D029968:Escherichia coli Proteins; C017168:cytochrome bo, E coli