[Determination of the folic acid content of vegetables and fruits using high-performance liquid chromatography (HPLC)].
The folic acid content of vegetables (including potatoes) and fruits was determined by means of high-performance liquid chromatography (HPLC). Free folate (monoglutamate forms) and total folate (monoglutamate+polyglutamate forms) are differentiated. Vegetables contained 27-187 micrograms/100 g and fruits 10-80 micrograms/100 g total folate. The free folate portion ranged from 6.3% to 96.8% (mean 35%) in vegetables and from 11.6% to 89.0% (mean 56%) in fruits. In addition, the results of the quantification of the main folate derivatives tetrahydrofolic acid (THF), 5-methyltetrahydrofolic acid (5-CH3-THF) and 5-formyltetrahydrofolic acid (5-CHO-THF) are presented. The mean values were 70% 5-CH3-THF, 20% 5-CHO-THF and 10% THF. Their total, resulting from the addition of pteroylglutamate equivalences, is comparable with the results of microbiological methods reported in the tables of nutritive values.
Müller
H
H
Institut für Ernährungsphysiologie, Bundesforschungsanstalt für Ernährung, Bundesrepublik Deutschland.
D002851:Chromatography, High Pressure Liquid; D005492:Folic Acid / Q000032:analysis*; D005638:Fruit / Q000737:chemistry*; D009753:Nutritive Value; D014675:Vegetables / Q000737:chemistry*
D005492:Folic Acid