title: Yeasts have a four-fold variation in ribosomal DNA copy number.
abstract: By employing pulsed-field gel electrophoresis we have determined the size of the rDNA cluster in wild-type yeast strains representing genera of Candida, Kluyveromyces, Pachysolen, Schizosaccharomyces and Torulaspora. Although the genome size of the examined species is similar (12.3-13.9 Mb), at least a four-fold variation has been observed between the lowest amount of rDNA repeats in P. tannophilus (28) and the highest in C. glabrata and S. poombe (> 115). In two species the rDNA cluster is represented by two loci, residing either in one (S. pombe) or two chromosomes (C. glabrata).
authors.affiliation: Molecular & Population Genetics Group, Research School of Biological Sciences, Australian National University, Canberra.
authors.forename: R
authors.initials: R
authors.lastname: Maleszka
authors.forename: G D
authors.initials: GD
authors.lastname: Clark-Walker
mesh_terms: D002175:Candida / Q000235:genetics; D015342:DNA Probes; D004271:DNA, Fungal / Q000235:genetics*; D004275:DNA, Ribosomal / Q000235:genetics*; D016521:Electrophoresis, Gel, Pulsed-Field; D016681:Genome, Fungal; D007716:Kluyveromyces / Q000235:genetics; D012568:Schizosaccharomyces / Q000235:genetics; D013045:Species Specificity; D015003:Yeasts / Q000235:genetics*
chemical_list: D015342:DNA Probes; D004271:DNA, Fungal; D004275:DNA, Ribosomal