Differential expression of the insulin-like growth factor II and transthyretin genes in the developing rat choroid plexus.
Choroid plexus (CP) development may depend on an inductive interaction between primordial CP epithelium and the overlying mesenchyme. Expression of the two CP epithelial-expressed genes, transthyretin (TTR) and insulin-like growth factor II (IGF-II), were studied by in situ hybridization in the developing rat. Transthyretin mRNA is expressed in abundance in the primordial CP epithelium prior to CP morphogenesis (e10-11) but IGF-II mRNA expression begins later (e13) and increases gradually as morphogenesis proceeds. In the CP stroma (mesenchyme), IGF-II mRNA is abundant prior to CP morphogenesis but decreases as embryogenesis proceeds and is absent in the adult. Our findings suggest that IGF-II may play an early paracrine and later autocrine role in CP development. A model is proposed in which IGF-II synthesized by mesenchyme serves as an inducing principle for CP epithelial differentiation.
Cavallaro
T
T
Department of Neurology, Columbia University, College of Physicians and Surgeons, New York, NY.
Martone
R L
RL
Stylianopoulou
F
F
Herbert
J
J
D000818:Animals; D002454:Cell Differentiation; D002831:Choroid Plexus / Q000166:cytology / Q000196:embryology* / Q000502:physiology; D005314:Embryonic and Fetal Development; D005260:Female; D015870:Gene Expression; D005865:Gestational Age; D017403:In Situ Hybridization; D007335:Insulin-Like Growth Factor II / Q000235:genetics*; D009024:Morphogenesis; D011228:Prealbumin / Q000235:genetics*; D011247:Pregnancy; D015347:RNA Probes; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley
D011228:Prealbumin; D015347:RNA Probes; D012333:RNA, Messenger; D007335:Insulin-Like Growth Factor II