Polymerase chain reaction for detection of Mycobacterium leprae in nasal swab specimens.
The polymerase chain reaction based on the selective amplification of a 531-bp fragment of the gene encoding the proline-rich antigen of Mycobacterium leprae was applied to nasal swab specimens from leprosy patients, occupational contacts, and endemic and nonendemic controls. To prevent false-positive amplification, we used dUTP and uracil-DNA-glycosylase in all polymerase chain reactions. False-negative reactions were detected by using a 531-bp modified template as an internal control. Amplification products were found in 55% of untreated patients, in 19% of the occupational contacts, in 12% of endemic controls, and in none of the nonendemic controls. This study strongly suggests that not only leprosy patients but also healthy persons may carry M. leprae. We concluded that polymerase chain reaction is a reliable method to detect M. leprae in nasal specimens. The method holds promise for studying the spread and transmission of M. leprae within a population.
de Wit
M Y
MY
N. H. Swellengrebel Laboratory of Tropical Hygiene, Royal Tropical Institute, Amsterdam, The Netherlands.
Douglas
J T
JT
McFadden
J
J
Klatser
P R
PR
D016358:Contact Tracing; D004797:Enzyme-Linked Immunosorbent Assay; D005189:False Positive Reactions; D006801:Humans; D007918:Leprosy / Q000453:epidemiology / Q000382:microbiology / Q000635:transmission; D009166:Mycobacterium leprae / Q000302:isolation & purification*; D009297:Nasal Mucosa / Q000382:microbiology*; D009426:Netherlands; D010679:Philippines; D016133:Polymerase Chain Reaction / Q000379:methods*; D013048:Specimen Handling