Composition of the phospholipids and their fatty acids in the ROC-1 oligodendroglial cell line.
ROC-1 cells are a hybrid of C-6 rat glioma and rat oligodendroglia cells. Biochemically these cells resemble the oligodendroglia parent, but their lipid composition is unknown. The phospholipid composition in mole % was: cardiolipin, 1.0; phosphatidylglycerol, 1.2; ethanolamine glycerophospholipids, 27.6; phosphatidylinositol, 5.8; lysophosphatidylethanolamine, 0.8; phosphatidylserine, 5.6; choline glycerophospholipids, 43.7; sphingomyelin, 13.7; phosphatidylinositol-4-monophosphate, 0.8; and lysophosphatidylcholine, 0.6. The choline and ethanolamine plasmalogens made up 7.2 and 18.4% of the total phospholipids, respectively. The phospholipid composition reflects that of both parental cells. The cells had moderate to high levels of 20:3n-9 indicating n-6 series fatty acid deficiency. The phosphatidylinositol had very high 20:3n-9 levels with a 20:3n-9/20:4n-6 ratio of 2.1 compared to 0.44 and 0.58 for ethanolamine glycerophospholipids (EtnGpl) and choline glycerophospholipids (ChoGpl) respectively. The saturated/polyenoic fatty acid ratios were 0.40 for EtnGpl, 3.38 for ChoGpl and 1.48 for phosphatidylinositol.
Murphy
E J
EJ
Department of Medical Biochemistry, Ohio State University, Columbus 43210.
Horrocks
L A
LA
D000818:Animals; D002460:Cell Line; D005227:Fatty Acids / Q000032:analysis* / Q000737:chemistry; D005231:Fatty Acids, Unsaturated / Q000032:analysis / Q000737:chemistry; D006822:Hybrid Cells / Q000737:chemistry; D009836:Oligodendroglia / Q000737:chemistry*; D010743:Phospholipids / Q000032:analysis* / Q000737:chemistry; D051381:Rats
D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated; D010743:Phospholipids