Comparison of intermittent injection of nondepolarizing solution with a single flush of UW solution for donor heart preservation.
Isolated canine hearts were preserved for 6 h at 5 degrees C followed by normothermic reperfusion for 2 h. The dogs were divided into two groups of nine hearts each; group 1 received a nondepolarizing preservation solution in multidose, and group 2 received a single flush of University of Wisconsin (UW) solution. Serum MB-CK and mitochondrial aspartate aminotransferase (m-AAT) concentrations and calcium overload during reperfusion were lower in group 1 than in group 2. At the end of reperfusion, myocardial ATP and total adenine nucleotide concentrations were higher and mitochondrial morphology appeared more intact in group 1 than in group 2. Left ventricular diastolic function was preserved better in group 1 than in group 2. These results suggest that in 6-h heart preservation, a nondepolarizing solution applied in multidose fashion protects the myocardium from the deleterious effects of hypothermia and cardioplegia better than a single flush of UW solution.
Miyamoto
H
H
Department of Thoracic-Cardiovascular Surgery, Tokyo Medical and Dental University, School of Medicine, Japan.
Sunamori
M
M
Suzuki
A
A
D000241:Adenosine; D000255:Adenosine Triphosphate / Q000378:metabolism; D000493:Allopurinol; D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism; D001794:Blood Pressure / Q000502:physiology; D002118:Calcium / Q000378:metabolism; D002314:Cardioplegic Solutions / Q000008:administration & dosage*; D003402:Creatine Kinase / Q000097:blood; D004285:Dogs; D005978:Glutathione; D006321:Heart; D006352:Heart Ventricles / Q000648:ultrastructure; D007267:Injections; D007328:Insulin; D008929:Mitochondria, Heart / Q000201:enzymology / Q000648:ultrastructure; D009926:Organ Preservation / Q000379:methods*; D019188:Organ Preservation Solutions; D010477:Perfusion; D011887:Raffinose; D012996:Solutions / Q000008:administration & dosage*; D014019:Tissue Donors; D016277:Ventricular Function, Left / Q000502:physiology
D002314:Cardioplegic Solutions; D007328:Insulin; D019188:Organ Preservation Solutions; D012996:Solutions; C055206:University of Wisconsin-lactobionate solution; D000493:Allopurinol; D000255:Adenosine Triphosphate; D001219:Aspartate Aminotransferases; D003402:Creatine Kinase; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose; D002118:Calcium