A rapid and inexpensive procedure for separation of high molecular weight DNA.
A pulse field gel electrophoresis procedure suitable for a variety of uses is described. The protocol has a run time of four hours. It was developed using field inversion technology and conventional gel boxes and is run at room temperature. This procedure provides a quick and inexpensive alternative for separation of high molecular weight DNA.
Centola
M B
MB
Department of Biological Sciences, University of California, Santa Barbara 93106.
D015825:Chromosomes, Fungal; D004271:DNA, Fungal / Q000302:isolation & purification*; D015246:Deoxyribonuclease EcoRI; D004586:Electrophoresis / Q000379:methods*; D015723:Gene Library; D008970:Molecular Weight; D009492:Neurospora crassa / Q000235:genetics*
D004271:DNA, Fungal; D015246:Deoxyribonuclease EcoRI