title: Sequencing two DNA templates in five channels by digital compression.
abstract: By applying algebraic coding methods to the Sanger dideoxynucleotide procedure, DNA sequences of two templates can be determined simultaneously in only five reactions and data channels. A 5:2 data compression is accomplished by instantaneous source coding of nucleotide sequence pairs into one set of 5-bit block codes. A general algebraic expression, 2n-1 > or = 4f, describes conditions under which f DNA templates can be sequenced using n channels. Such compression sequencing is accurate and efficient, as demonstrated by manual 35S autoradiographic detection and automated on-line analysis using fluorescent-labeled primers. Symmetric 5:2 compression is especially useful when comparing two closely related sequences.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Chicago, IL 60637.
authors.forename: M
authors.initials: M
authors.lastname: Nelson
authors.forename: Y
authors.initials: Y
authors.lastname: Zhang
authors.forename: D L
authors.initials: DL
authors.lastname: Steffens
authors.forename: R
authors.initials: R
authors.lastname: Grabherr
authors.forename: J L
authors.initials: JL
authors.lastname: Van Etten
mesh_terms: D001331:Automation; D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D005796:Genes; D011544:Pseudogenes; D013698:Templates, Genetic
chemical_list: D004247:DNA; D004259:DNA-Directed DNA Polymerase