title: Circulating cellular immune response to oral immunization of humans with cholera toxin B-subunit.
abstract: Peripheral blood mononuclear cells were taken from subjects before and after oral immunization with cholera toxin B-subunit. Cells obtained from naive volunteers before immunization did not proliferate in vitro to B-subunit. Oral immunization induced a proliferative response in all volunteers with a peak stimulation index of 20, and was detected up to 1 year later. The proliferative response kinetics suggest the appearance in the blood of primed T cells from the gut coinciding with the disappearance of primed plasmablasts from the circulation, supporting the concept of a common mucosal immune system in man for T and B cells.
authors.affiliation: Division of Communicable Diseases, St George's Hospital Medical School, London, UK.
authors.forename: D J
authors.initials: DJ
authors.lastname: Lewis
authors.forename: L R
authors.initials: LR
authors.lastname: Castello-Branco
authors.forename: P
authors.initials: P
authors.lastname: Novotny
authors.forename: G
authors.initials: G
authors.lastname: Dougan
authors.forename: T A
authors.initials: TA
authors.lastname: Poulton
authors.forename: G E
authors.initials: GE
authors.lastname: Griffin
mesh_terms: D000284:Administration, Oral; D000328:Adult; D002772:Cholera Toxin / Q000276:immunology*; D022121:Cholera Vaccines / Q000008:administration & dosage / Q000276:immunology*; D005260:Female; D006801:Humans; D007111:Immunity, Cellular; D007413:Intestinal Mucosa / Q000276:immunology; D008213:Lymphocyte Activation; D008297:Male; D013601:T-Lymphocytes / Q000276:immunology*; D014611:Vaccination / Q000379:methods*; D015164:Vaccines, Inactivated / Q000008:administration & dosage / Q000276:immunology; D014734:Vibrio cholerae / Q000276:immunology
chemical_list: D022121:Cholera Vaccines; D015164:Vaccines, Inactivated; D002772:Cholera Toxin