Characterization of neutrophil activation by repeated injection of endotoxin in rabbits. Role of neutrophils in the generalized Shwartzman reaction.
The relationship between activated neutrophils and end-organ injury in endotoxemia was studied. The function of peripheral blood neutrophils (PMNs) in rabbits with the generalized Shwartzman reaction (GSR) was compared to that of PMNs rabbits receiving a single injection of endotoxin. The following results were obtained: (1) PMNs from rabbits with the GSR demonstrated enhanced adherence to endothelial cells and increased mitochondrial ATP production; (2) the GSR did not enhance chemotaxis and oxygen radical production of PMNs; (3) a single injection of endotoxin did not cause necrosis of visceral organs; (4) in vitro detachment of endothelial cells by PMNs was increased in rabbits with the GSR; (5) in vivo administration of monoclonal antibody (mAb) against CD11b/CD18 (Mac-1) suppressed the increase in PMN adherence; and (6) hemorrhagic necrosis did not occur when mAb to Mac-1 was injected. Thus, enhanced adherence of PMNs to endothelial cells appears to play a key role in endotoxin-induced end-organ injuries in this animal model.
Toh
H
H
First Department of Surgery, Kyushu University School of Medicine, Fukuoka, Japan.
Miyata
T
T
Torisu
M
M
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D002448:Cell Adhesion; D002634:Chemotaxis, Leukocyte; D004727:Endothelium / Q000166:cytology; D004731:Endotoxins / Q000494:pharmacology*; D007275:Injections, Intravenous; D008099:Liver / Q000473:pathology; D008163:Luminescent Measurements; D008168:Lung / Q000473:pathology; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D011817:Rabbits; D012790:Shwartzman Phenomenon / Q000473:pathology / Q000503:physiopathology*; D013778:Tetrazolium Salts; D013844:Thiazoles
D000911:Antibodies, Monoclonal; D004731:Endotoxins; D013778:Tetrazolium Salts; D013844:Thiazoles; C022616:thiazolyl blue