title: Divergent T cell receptor gamma repertoires in rheumatoid arthritis monozygotic twins.
abstract: OBJECTIVE
  To determine if the expressed T cell receptor (TCR) gamma repertoire is altered in rheumatoid arthritis (RA).
  
  
  METHODS
  Peripheral blood lymphocytes were collected from monozygotic twins who were either concordant or discordant for RA, or from a normal twin pair. TCR gamma-specific complementary DNA libraries were constructed using the anchored polymerase chain reaction. Gene usage was analyzed by plaque hybridization and sequencing.
  
  
  RESULTS
  The expressed TCR V gamma repertoires both in RA patients and normal subjects were extremely diverse. Monozygotic twins who were concordant for RA expressed very different frequencies of TCR V gamma genes.
  
  
  CONCLUSIONS
  RA does not lead to a specific clonal expansion or deletion of TCR V gamma genes in peripheral blood.
authors.affiliation: Sam and Rose Stein Institute for Research on Aging, University of California, San Diego, La Jolla 92093-0663.
authors.forename: H
authors.initials: H
authors.lastname: Kohsaka
authors.forename: P P
authors.initials: PP
authors.lastname: Chen
authors.forename: A
authors.initials: A
authors.lastname: Taniguchi
authors.forename: W E
authors.initials: WE
authors.lastname: Ollier
authors.forename: D A
authors.initials: DA
authors.lastname: Carson
mesh_terms: D000328:Adult; D001172:Arthritis, Rheumatoid / Q000235:genetics*; D001483:Base Sequence; D004200:Diseases in Twins / Q000235:genetics*; D005260:Female; D005784:Gene Amplification; D006801:Humans; D008213:Lymphocyte Activation / Q000235:genetics; D008875:Middle Aged; D008969:Molecular Sequence Data; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000235:genetics*; D017422:Sequence Analysis, DNA; D014158:Transcription, Genetic; D014430:Twins, Monozygotic
chemical_list: D016692:Receptors, Antigen, T-Cell, gamma-delta