A somatic cell hybrid map of the long arm of human chromosome 17, containing the familial breast cancer locus (BRCA1).
We describe a detailed somatic cell hybrid map of human chromosome 17q11.2-q23, containing the familial breast and ovarian cancer locus (BRCA1) and highly informative closely linked markers. An X-irradiation panel of 38 hamster/human and mouse/human hybrids with fragments of chromosome 17 was generated and characterized with 22 STS markers from this chromosome. A detailed map of 61 probes onto chromosome 17q, subdividing the chromosome arm into 25 regions, was done by using a panel of hybrids with well-defined breakpoints and nine chromosome-mediated gene transfectants. Our localization of RARA, TOP2, EDH17B1 and 2, and possibly WNT3, between THRA1 and D17S181, two markers known to flank BRCA1, suggests that any of these is a potential candidate for the BRCA1 locus. The marker D17S579 (Mfd188), which is believed to be very close to BRCA1, maps closest to the EDH17B genes.
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Somatic Cell Genetics Laboratory, Imperial Cancer Research Fund, Lincoln's Inn Fields, London, England.
Nicolai
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Borrow
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Solomon
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D000818:Animals; D015139:Blotting, Southern; D001943:Breast Neoplasms / Q000235:genetics*; D002459:Cell Fusion; D002461:Cell Line, Transformed; D002874:Chromosome Mapping / Q000379:methods*; D002886:Chromosomes, Human, Pair 17; D006224:Cricetinae; D015342:DNA Probes; D004273:DNA, Neoplasm / Q000032:analysis; D005192:Family Health; D005260:Female; D006801:Humans; D006822:Hybrid Cells / Q000528:radiation effects; D051379:Mice; D009386:Neoplastic Syndromes, Hereditary / Q000235:genetics; D010051:Ovarian Neoplasms / Q000235:genetics; D016133:Polymerase Chain Reaction; D011519:Proto-Oncogenes; D016324:Sequence Tagged Sites; D014162:Transfection
D015342:DNA Probes; D004273:DNA, Neoplasm