Expression of sulfated glycoprotein 2 is associated with carcinogenesis induced by N-nitroso-N-methylurea in rat prostate and seminal vesicle.
To understand the molecular mechanism of carcinogenesis in androgen-dependent tumors, we have searched for new markers which are associated with this process. In normal rat prostate and seminal vesicle, sulfated glycoprotein 2 (SGP-2) messenger RNA is barely detectable. However, we have found high levels of SGP-2 expression in the epithelial component of carcinomas of the prostate and seminal vesicle after initiation with N-nitroso-N-methylurea and promotion with testosterone propionate. We have also observed induction of SGP-2 expression in epithelial cells at early stages in carcinogenesis when cytologically malignant cells first begin to appear. SGP-2 has been reported previously to be associated with a variety of models of programmed cell death (apoptosis), including the prostate following castration. Our present findings provide a novel marker for carcinogenesis in the rat prostate and seminal vesicle.
Kadomatsu
K
K
Laboratory of Chemoprevention, National Cancer Institute, Bethesda, Maryland 20892.
Anzano
M A
MA
Slayter
M V
MV
Winokur
T S
TS
Smith
J M
JM
Sporn
M B
MB
D000818:Animals; D014408:Biomarkers, Tumor / Q000378:metabolism*; D015152:Blotting, Northern; D051152:Clusterin; D004848:Epithelium / Q000378:metabolism; D006023:Glycoproteins / Q000378:metabolism*; D008297:Male; D008770:Methylnitrosourea; D018832:Molecular Chaperones; D011471:Prostatic Neoplasms / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D012669:Seminal Vesicles / Q000378:metabolism*
D014408:Biomarkers, Tumor; D051152:Clusterin; D006023:Glycoproteins; D018832:Molecular Chaperones; D008770:Methylnitrosourea