5-Lipoxygenase gene expression in HL60 cells during differentiation with DMSO.
The model of promyelocytic leukemia cells HL60 was utilized to study the accumulation of 5-lipoxygenase mRNA during the cellular differentiation induced by DMSO. In the present study a strict correlation between the increase in state of differentiation of HL60 and accumulation of 5-lipoxygenase mRNA is demonstrated, indicating that HL60 cells are an interesting model for the study of drugs which could interfere with the synthesis of the enzyme.
Piechele
G
G
Institute of Pharmacological Sciences, University of Milan, Italy.
Colli
S
S
Tremoli
E
E
Maggi
A
A
D001094:Arachidonate 5-Lipoxygenase / Q000096:biosynthesis* / Q000187:drug effects / Q000235:genetics; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D006801:Humans; D015473:Leukemia, Promyelocytic, Acute; D009504:Neutrophils / Q000378:metabolism*; D012333:RNA, Messenger / Q000187:drug effects; D014407:Tumor Cells, Cultured
D012333:RNA, Messenger; D001094:Arachidonate 5-Lipoxygenase; D004121:Dimethyl Sulfoxide