Rapid production of a panel of blood group A-active oligosaccharides using chemically synthesized di- and tri-saccharide primers and an easily prepared porcine (1-->3)-alpha-N-acetyl-D-galactosaminyltransferase.
A porcine (1-->3)-alpha-N-acetyl-D-galactosaminyltransferase was obtained in a state suitable for preparative-scale (mg-scale) synthesis using simple procedures requiring only three days of effort. The enzyme thus prepared transferred GalNAc efficiently from UDP-GalNAc to six different chemically synthesized di- and tri-saccharide H-active structures to yield blood-group A-active oligosaccharides that were characterized by 1H NMR spectroscopy and mass spectrometry. This work further demonstrates the efficiency and attractiveness of using glycosyltransferases in a combined chemoenzymatic approach for the rapid production of biologically active oligosaccharides.
Compston
C A
CA
Chembiomed Ltd., Edmonton, Alberta, Canada.
Condon
C
C
Hanna
H R
HR
Mazid
M A
MA
D000017:ABO Blood-Group System / Q000737:chemistry*; D000818:Animals; D002240:Carbohydrate Sequence; D004187:Disaccharides / Q000737:chemistry; D005753:Gastric Mucosa / Q000201:enzymology*; D006031:Glycosylation; D008969:Molecular Sequence Data; D017350:N-Acetylgalactosaminyltransferases / Q000302:isolation & purification / Q000378:metabolism*; D009844:Oligosaccharides / Q000737:chemistry*; D013552:Swine; D014312:Trisaccharides / Q000737:chemistry
D000017:ABO Blood-Group System; D004187:Disaccharides; D009844:Oligosaccharides; D014312:Trisaccharides; D017350:N-Acetylgalactosaminyltransferases; C003742:UDPgalactosamine-galactose acetylgalactosaminyltransferase