title: Stable expression and regulation of a rat brain K+ channel.
abstract: The Shaw-type K+ channel Kv3.1 was stably transfected in human embryonic kidney cells. Voltage dependence of activation, K+ permeability, sensitivity to external tetraethylammonium, and unitary conductance were similar to Kv3.1 channels expressed transiently in Xenopus oocytes. Kv3.1 channels appear to be regulated because the protein kinase C activator phorbol 12,13-dibutyrate decreased Kv3.1 currents. Based on these results, we find that the stable expression of voltage-gated K+ channels in human embryonic kidney cells appears to be well suited for analysis of both biophysical and biochemical regulatory processes.
authors.affiliation: Department of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, Texas 77030.
authors.forename: S D
authors.initials: SD
authors.lastname: Critz
authors.forename: B A
authors.initials: BA
authors.lastname: Wible
authors.forename: H S
authors.initials: HS
authors.lastname: Lopez
authors.forename: A M
authors.initials: AM
authors.lastname: Brown
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D002461:Cell Line, Transformed; D004594:Electrophysiology; D004789:Enzyme Activation; D006801:Humans; D007668:Kidney / Q000166:cytology / Q000378:metabolism / Q000502:physiology; D015240:Phorbol 12,13-Dibutyrate / Q000494:pharmacology; D015221:Potassium Channels / Q000378:metabolism* / Q000502:physiology; D011493:Protein Kinase C / Q000378:metabolism; D051381:Rats; D014162:Transfection
chemical_list: D015221:Potassium Channels; D015240:Phorbol 12,13-Dibutyrate; D011493:Protein Kinase C