Circadian expression and induction by wounding of tobacco genes for cysteine proteinase.
Two sets of clones were isolated from a tobacco cDNA library, utilizing as a probe a PCR fragment obtained from tomato cDNA using a degenerate primer based on the sequence of tomato systemin. Contrary to expectation, the clones did not correspond to tobacco homologues of tomato pro-systemin. However, the cDNAs encoded two highly similar proteins with extensive structural homology to cysteine proteinases from a wide range of plant and animal species. Northern blot analyses showed that in unstressed tobacco leaf the genes for the putative proteinases are expressed according to a circadian rhythm. Furthermore, incision wounding enhances the expression approximately six-fold. Other forms of stress, such as infection with tobacco mosaic virus, treatment with ethephon or UV light do not result in induced expression of the tobacco cysteine proteinase genes.
Linthorst
H J
HJ
Department of Biochemistry, Leiden University, Netherlands.
van der Does
C
C
Brederode
F T
FT
Bol
J F
JF
D000595:Amino Acid Sequence; D001483:Base Sequence; D002940:Circadian Rhythm; D003001:Cloning, Molecular; D003546:Cysteine Endopeptidases / Q000235:genetics*; D004247:DNA / Q000235:genetics; D005786:Gene Expression Regulation; D017343:Genes, Plant; D006360:Heat-Shock Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics; D010947:Plants, Toxic; D012333:RNA, Messenger / Q000235:genetics; D016415:Sequence Alignment; D014026:Nicotiana / Q000235:genetics*; D014945:Wound Healing
D006360:Heat-Shock Proteins; D010940:Plant Proteins; D012333:RNA, Messenger; D004247:DNA; D003546:Cysteine Endopeptidases