Effects of midazolam on insulin secretion from isolated rat pancreatic islets of Langerhans.
Isolated, cultured rat pancreatic islets of Langerhans were incubated in medium containing glucose 2 or 20 mmol litre-1 and with glucose supplemented with midazolam 10, 100 or 1000 ng ml-1. Glucose 20 mmol litre-1 with the addition of noradrenaline 10 mumol litre-1 was used as a control. In contrast with noradrenaline, midazolam did not inhibit glucose-induced insulin secretion. Noradrenaline 10 mumol litre-1 inhibited secretion to basal values in response to glucose 20 mmol litre-1 (P < 0.001).
Desborough
J P
JP
Department of Anaesthetics, Royal Postgraduate Medical School, Hammersmith Hospital, London.
Jones
P M
PM
Persaud
S J
SJ
Howell
S L
SL
D000818:Animals; D002478:Cells, Cultured; D005947:Glucose / Q000037:antagonists & inhibitors / Q000494:pharmacology; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000187:drug effects / Q000378:metabolism*; D008874:Midazolam / Q000494:pharmacology*; D009638:Norepinephrine / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar
D007328:Insulin; D005947:Glucose; D008874:Midazolam; D009638:Norepinephrine