title: C-terminal truncation of the transcriptional activator encoded by areA in Aspergillus nidulans results in both loss-of-function and gain-of-function phenotypes.
abstract: Mutations truncating as many as 143 C-terminal residues from the transcriptional activator encoded by the areA gene, mediating nitrogen metabolite repression in Aspergillus nidulans, do not significantly reduce the ability of the areA product to activate expression of most genes under areA control. Such mutations can even have a gain-of-function, derepressed phenotype, consistent with a critical role for this region in modulating the activity of the areA protein. However, expression of a few genes under areA control is substantially impaired by such C-terminal truncations, indicating that regions of an activator protein can play differing roles in the control of different structural genes. This underlines the advantages of being able to monitor effects of areA mutations on expression of large numbers of structural genes. Additionally, it is shown that truncation of as many as 153 C-terminal residues, virtually all amino acids C-terminal to the DNA-binding region, is compatible with retention of some areA function.
authors.affiliation: Department of Chemistry, University of San Diego, California 92110-2492.
authors.forename: M
authors.initials: M
authors.lastname: Stankovich
authors.forename: A
authors.initials: A
authors.lastname: Platt
authors.forename: M X
authors.initials: MX
authors.lastname: Caddick
authors.forename: T
authors.initials: T
authors.lastname: Langdon
authors.forename: P M
authors.initials: PM
authors.lastname: Shaffer
authors.forename: H N
authors.initials: HN
authors.lastname: Arst
mesh_terms: D000595:Amino Acid Sequence; D001233:Aspergillus nidulans / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D010641:Phenotype; D017384:Sequence Deletion; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic; D015533:Transcriptional Activation
chemical_list: C079728:AreA protein, Aspergillus nidulans; D005656:Fungal Proteins; D014157:Transcription Factors