title: Purification of recombinant hepatitis delta antigen expressed in E. coli cells.
abstract: Recombinant DNA technology enables the massive production of recombinant hepatitis delta antigen (recHDAg) retaining immunological properties and transport functions. However, purification procedures of the recombinant delta antigen have, to date, not been described in the literature. We present a purification procedure allowing one to obtain highly purified recHDAg from bacterial cells expressing the hepatitis delta antigen.
authors.affiliation: Dipartimento di Genetica, Biologia Generale e Molecolare, Università di Napoli FedericoII, Italy.
authors.forename: R
authors.initials: R
authors.lastname: Calogero
authors.forename: U
authors.initials: U
authors.lastname: Barbieri
authors.forename: M
authors.initials: M
authors.lastname: Borla
authors.forename: S
authors.initials: S
authors.lastname: Osborne
authors.forename: F
authors.initials: F
authors.lastname: Poisson
authors.forename: F
authors.initials: F
authors.lastname: Bonelli
mesh_terms: D000956:Antigens, Viral / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism*; D003698:Hepatitis Delta Virus / Q000235:genetics / Q000276:immunology*; D012367:RNA, Viral / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D001616:beta-Galactosidase
chemical_list: D000956:Antigens, Viral; D012367:RNA, Viral; D011993:Recombinant Fusion Proteins; D001616:beta-Galactosidase