Effects of adenosine and its analogues on actin polymerization in human polymorphonuclear leucocytes.
1. The effects of adenosine and its analogues on actin polymerization in human polymorphonuclear leucocytes (PMN) induced by three different chemotactic stimulants, platelet-activating factor (PAF), N-formyl-methionyl-leucyl-phenylalanine (FMLP) and an activated fragment of C5 (C5a) were investigated. 2. Adenosine and its analogues inhibited the actin polymerization induced by these three agents in a concentration-dependent manner and theophylline, a competitive antagonist at adenosine receptors, abolished these inhibitory effects. 3. The adenosine analogue 5'-N-ethylcarboxamideadenosine (NECA) was a more potent inhibitor of actin polymerization than either L-N6-phenylisopropyladenosine (PIA) or adenosine itself; the rank order of potency of these agonists was characteristic of adenosine A2 receptors. 4. Adenosine deaminase (ADA) abolished the inhibitory effect of adenosine and augmented PAF-induced actin polymerization. 5. It was concluded that, at physiological concentrations, adenosine inhibits actin polymerization in PMN via activation of PMN surface membrane adenosine A2 receptors and thus modulates chemotactic stimulus-induced PMN motility.
Tsuruta
S
S
Department of Pediatrics, Kyoto University Hospital, Japan.
Ito
S
S
Mikawa
H
H
D000199:Actins / Q000097:blood* / Q000187:drug effects*; D000241:Adenosine / Q000031:analogs & derivatives* / Q000494:pharmacology*; D000243:Adenosine Deaminase / Q000097:blood; D001704:Biopolymers; D015936:Complement C5a / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism; D015649:Pentostatin / Q000494:pharmacology; D010972:Platelet Activating Factor / Q000494:pharmacology; D013806:Theophylline / Q000494:pharmacology
D000199:Actins; D001704:Biopolymers; D010972:Platelet Activating Factor; D015649:Pentostatin; D009240:N-Formylmethionine Leucyl-Phenylalanine; D015936:Complement C5a; D013806:Theophylline; D000243:Adenosine Deaminase; D000241:Adenosine