A method for recovery of high-molecular-weight DNA suitable for field-inversion gel electrophoresis from frozen tumor cells.
We describe a simple method for isolation of high-molecular-weight (high-mol-wt, > 800 kilobases) DNA from tumor cell lines frozen without dimethylsulfoxide (DMSO) or other protective agents. This method should be applicable to most frozen tissues and useful for molecular genetic studies such as field-inversion gel electrophoresis (FIGE), or cloning procedures that require access to intact, high-mol-wt DNA.
Reynolds
J E
JE
Department of Human Genetics, Medical College of Virginia, Virginia Commonwealth University, Richmond 23298-0710.
Diehl
S R
SR
D015925:Cryopreservation; D004273:DNA, Neoplasm / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel / Q000379:methods; D005615:Freezing; D006801:Humans; D008654:Mesothelioma / Q000235:genetics*; D014407:Tumor Cells, Cultured
D004273:DNA, Neoplasm