[Clinical study on the cell kinetics of gastric cancer using bromodeoxyuridine labeling index--its relations with DNA ploidy pattern and epidermal growth factor].
Forty fresh gastric cancer specimens were examined by immunohistological staining using anti-BrdU monoclonal antibody, and simultaneously the specimens were studied by DNA ploidy pattern, EGF with flowcytometer and the immunohistological staining. For EGF study, the same 40 gastric cancer formalin fixed specimens were used. The results of flowcytometric measurement were divided into diploid and aneuploid patterns. BrdU positive stained cancer cells were observed in growing border area than in the center of tumor, and histologically in P(+), n(+), ps(+), INF gamma, and in scirrhous type, deep spread type and advanced stage. This data suggested that the BrdU labeling index seemed to be related to invasion, proliferation, and metastasis of cancer cells. However the positive rates of EGF were higher in ps(+), deep spread type and BrdU positive type but not in P(+), n(+), and EGF was considered to related to invasion but not to be related to metastasis. Although aneuploid pattern cancer showed high BrdU labeling index in ps(+), diploid pattern didn't indicate such tendency. High BrdU positive rate aneuploid cancer were seemed to grow quickly, advance in short period and own worse character. Further investigation would be necessary.
Tsushima
H
H
Department of Surgery, Showa University School of Medicine, Tokyo, Japan.
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000911:Antibodies, Monoclonal; D001973:Bromodeoxyuridine / Q000276:immunology; D002453:Cell Cycle; D004273:DNA, Neoplasm / Q000032:analysis*; D004815:Epidermal Growth Factor / Q000032:analysis; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D011003:Ploidies; D013274:Stomach Neoplasms / Q000235:genetics / Q000473:pathology*
D000911:Antibodies, Monoclonal; D004273:DNA, Neoplasm; D004815:Epidermal Growth Factor; D001973:Bromodeoxyuridine