Chromatographic separation of glycated nucleotides.
A high-performance liquid chromatographic method for the separation of nucleotide glycation reaction product(s) has been developed. The product(s) arising during in vitro glycation of a nucleotide (AMP, GMP or CMP) with a reducing sugar (ribose or glucose) were clearly resolved from the non-glycated constituents of the reaction mixture. The peak(s) of the glycated product(s) increased when an amino acid (lysine, arginine, beta-alanine or N epsilon-acetyllysine) was added to the reaction mixture. This increase probably corresponds to the formation of a new product with a different absorption maximum (250 versus 260 nm). Conversely, formation of this product(s) was inhibited by the presence of the metal-chelating agent diethylenetriamine pentaacetic acid and by aminoguanidine.
Miksík
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I
Institute of Physiology, Czechoslovak Academy of Sciences, Prague.
Hodný
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Z
Deyl
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D000596:Amino Acids / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D005947:Glucose / Q000737:chemistry; D005985:Glyceraldehyde / Q000737:chemistry; D006146:Guanidines; D007202:Indicators and Reagents; D009711:Nucleotides / Q000737:chemistry / Q000302:isolation & purification*; D004369:Pentetic Acid; D012266:Ribose / Q000737:chemistry
D000596:Amino Acids; D006146:Guanidines; D007202:Indicators and Reagents; D009711:Nucleotides; D005985:Glyceraldehyde; D012266:Ribose; D004369:Pentetic Acid; D005947:Glucose; C004479:pimagedine