Non-transferrin-bound-iron in serum and low-molecular-weight-iron in the liver of dietary iron-loaded rats.
1. The feeding of 0.5% (3,5,5-trimethylhexanoyl)ferrocene (TMH-ferrocene) in rats resulted in a severe and progressive liver siderosis (total liver iron, 30 mg/g liver wet weight, after 30 weeks). 2. High concentrations of an iron-rich ferritin (up to 250 mg/l) were detected in serum of heavily iron-loaded rats forming a large fraction of non-transferrin-bound-iron (5000 micrograms/dl in maximum). 3. Ferritin and not haemosiderin was the major iron storage protein in the liver. 4. The total liver iron concentration (from 0.4 to > 30 mg Fe/g wet wt) but not the cytosolic low-molecular-weight-iron fraction (from 0.5 to 2.5 microM) was extremely increased during iron-loading.
Nielsen
P
P
Abtlg Medizinische Biochemie, Physiologisch-Chemisches Institut, Universitätskrankenhaus Eppendorf, Hamburg, Germany.
Düllmann
J
J
Wulfhekel
U
U
Heinrich
H C
HC
D000818:Animals; D003600:Cytosol / Q000378:metabolism; D004032:Diet; D005260:Female; D005293:Ferritins / Q000378:metabolism; D005296:Ferrous Compounds / Q000008:administration & dosage; D006485:Hemosiderin / Q000378:metabolism; D007501:Iron / Q000008:administration & dosage* / Q000097:blood / Q000378:metabolism*; D008099:Liver / Q000378:metabolism* / Q000648:ultrastructure; D000075163:Metallocenes; D008854:Microscopy, Electron; D008970:Molecular Weight; D009942:Organometallic Compounds / Q000008:administration & dosage; D051381:Rats; D017208:Rats, Wistar; D013054:Spectrophotometry, Atomic; D014168:Transferrin / Q000378:metabolism*
D005296:Ferrous Compounds; D000075163:Metallocenes; D009942:Organometallic Compounds; D014168:Transferrin; C050224:3,5,5-trimethylhexanoylferrocene; D005293:Ferritins; D006485:Hemosiderin; D007501:Iron