Biosynthesis of 12(S)-hydroxyeicosatetraenoic acid by bovine corneal epithelium.
Microsomes of bovine corneal epithelium metabolized [14C]arachidonic acid extensively to [14C]12-hydroxyeicosatetraenoic acid. The biosynthesis was not stimulated by exogenous NADPH and it could be inhibited by over 90% with two lipoxygenase inhibitors, esculetin and eicosatetraynoic acid. Chiral phase high performance liquid chromatography showed that 12-HETE consisted of the 12S enantiomer. The results indicate biosynthesis by 12S-lipoxygenase of bovine corneal epithelium rather than by cytochrome P450.
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Department of Pharmaceutical Pharmacology, Uppsala University Biomedical Centre, Sweden.
D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; D015117:5,8,11,14-Eicosatetraynoic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D004728:Endothelium, Corneal / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D006893:Hydroxyeicosatetraenoic Acids / Q000096:biosynthesis*; D066298:In Vitro Techniques; D008084:Lipoxygenase / Q000378:metabolism; D016859:Lipoxygenase Inhibitors / Q000494:pharmacology; D008861:Microsomes / Q000378:metabolism; D008968:Molecular Conformation; D009249:NADP / Q000494:pharmacology; D014468:Umbelliferones / Q000494:pharmacology
D006893:Hydroxyeicosatetraenoic Acids; D016859:Lipoxygenase Inhibitors; D014468:Umbelliferones; D015117:5,8,11,14-Eicosatetraynoic Acid; D009249:NADP; D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; C029510:4,7,10,13-eicosatetraynoic acid; D003577:Cytochrome P-450 Enzyme System; D008084:Lipoxygenase; C007628:esculetin