High-performance liquid chromatography of human glycoprotein hormones.
The chromatographic behavior of the glycoprotein hormones from human pituitary glands and of placental origin [thyroid-stimulating hormone, luteinizing hormone and chorionic gonadotropin (CG)] was studied. It was shown that hydrophobic interaction chromatography on a microparticulate packing and anion-exchange HPLC can be applied for the purification of these hormones. Reversed-phase HPLC on wide-pore C4-bonded silica at neutral pH can be applied for the determination of the above hormones and for the isolation of pure CG and its subunits.
Chlenov
M A
MA
NPO "Biotechnologia", Moscow, Russian Federation.
Kandyba
E I
EI
Nagornaya
L V
LV
Orlova
I L
IL
Volgin
Y V
YV
D006063:Chorionic Gonadotropin / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002852:Chromatography, Ion Exchange; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007986:Luteinizing Hormone / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet; D013972:Thyrotropin / Q000032:analysis*
D006063:Chorionic Gonadotropin; D007986:Luteinizing Hormone; D013972:Thyrotropin