Detection of a plant protein analogous to the yeast spliceosomal protein, PRP8.
We have investigated whether a spliceosomal protein analogous to the yeast protein, PRP8, was present in higher plants. A protein with a molecular weight > 200 kDa was detected in Western blots of tobacco (Nicotiana tabacum L.) nuclear extracts with affinity-purified antibodies, raised against four different beta-galactosidase-PRP8 fusion proteins. The < 200 kDa protein was also immunoprecipitated by antibodies against the snRNA-specific trimethylguanosine cap structure and was, therefore, snRNP-associated. The presence of this protein in plants, in addition to yeast, Drosophila and humans, and the conservation of large size and epitopes highlights the importance of PRP8 in pre-mRNA splicing.
Kulesza
H
H
Cellular and Molecular Genetics Department, Scottish Crop Research Institute, Invergowrie, Dundee, UK.
Simpson
G G
GG
Waugh
R
R
Beggs
J D
JD
Brown
J W
JW
D015153:Blotting, Western; D003429:Cross Reactions; D005656:Fungal Proteins / Q000032:analysis* / Q000737:chemistry; D010940:Plant Proteins / Q000032:analysis* / Q000737:chemistry; D010947:Plants, Toxic; D011233:Precipitin Tests; D012342:RNA, Small Nuclear / Q000302:isolation & purification; D017414:Ribonucleoprotein, U4-U6 Small Nuclear; D017415:Ribonucleoprotein, U5 Small Nuclear; D012441:Saccharomyces cerevisiae / Q000737:chemistry*; D029701:Saccharomyces cerevisiae Proteins; D017381:Spliceosomes / Q000737:chemistry*; D014026:Nicotiana / Q000737:chemistry*
D005656:Fungal Proteins; C077018:PRP8 protein, S cerevisiae; D010940:Plant Proteins; D012342:RNA, Small Nuclear; D017414:Ribonucleoprotein, U4-U6 Small Nuclear; D017415:Ribonucleoprotein, U5 Small Nuclear; D029701:Saccharomyces cerevisiae Proteins