title: Toxin detection using a tyrosinase-coupled oxygen electrode.
abstract: An enzyme-based "electrochemical canary" is described for the detection of cyanide. The sensing system imitates cyanide's site of toxicity in the mitochondria. The terminal sequence of electron transfer in aerobic respiration is mimicked by mediator coupling of tyrosinase catalysis to an electro-chemical system. An enzyme-coupled oxygen electrode is created which is sensitive to selective poisoning. Biocatalytic reduction of oxygen is promoted by electrochemically supplying tyrosinase with electrons. Thus, ferrocyanide is generated at a cathode and mediates the enzymatic reduction of oxygen to water. An enzyme-dependent reductive current can be monitored which is inhibited by cyanide in a concentration-dependent manner. Oxygen depletion in the reaction layer can be minimized by addressing enzyme activity using a potential pulsing routine. Enzyme activity is electrochemically initiated and terminated and the sensor becomes capable of continuous monitoring. Cyanide poisoning of the biological component is reversible, and it can be reused after rinsing. The resulting sensor detects cyanide based on its biological activity rather than its physical or chemical properties.
authors.affiliation: Hawaii Biosensor Laboratory, Department of Chemistry, University of Hawaii, Honolulu 96822.
authors.forename: M H
authors.initials: MH
authors.lastname: Smit
authors.forename: G A
authors.initials: GA
authors.lastname: Rechnitz
mesh_terms: D015374:Biosensing Techniques; D002384:Catalysis; D003486:Cyanides / Q000032:analysis*; D014442:Monophenol Monooxygenase / Q000737:chemistry*; D010084:Oxidation-Reduction
chemical_list: D003486:Cyanides; D014442:Monophenol Monooxygenase