Differential adrenergic regulation of C/EBP alpha and C/EBP beta in brown adipose tissue.
We investigated the regulation of the expression of two members of the C/EBP family of transcriptional activators, C/EBP alpha and C/EBP beta, in brown adipose tissue in mice. Less than one hour of cold exposure led to dramatic changes in the expression of both genes. C/EBP alpha steady-state mRNA and protein levels were drastically and rapidly reduced whereas C/EBP beta mRNA and protein levels were induced severalfold. Also norepinephrine injection affected the expression of the transcription factors. Preconfluent cells in brown fat primary cultures responded to norepinephrine with a decrease in C/EBP alpha and an increase in C/EBP beta mRNA; in confluent cells the expression of both factors was increased. Thus, C/EBP alpha and C/EBP beta gene expression is under adrenergic control both in vivo and in vitro but the type of response is directed by the degree of differentiation of the cells.
Rehnmark
S
S
Wenner-Gren Institute, Arrhenius Laboratories F3, Stockholm University, Sweden.
Antonson
P
P
Xanthopoulos
K G
KG
Jacobsson
A
A
D002001:Adipose Tissue, Brown / Q000378:metabolism*; D000818:Animals; D022762:CCAAT-Enhancer-Binding Proteins; D002454:Cell Differentiation; D002478:Cells, Cultured; D003080:Cold Temperature; D004268:DNA-Binding Proteins / Q000235:genetics*; D005786:Gene Expression Regulation / Q000187:drug effects*; D015151:Immunoblotting; D051379:Mice; D009638:Norepinephrine / Q000494:pharmacology*; D009687:Nuclear Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics
D022762:CCAAT-Enhancer-Binding Proteins; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D012333:RNA, Messenger; D014157:Transcription Factors; D009638:Norepinephrine