title: Purification and immunochemical characterization of a male-specific rat liver oestrogen sulphotransferase.
abstract: Sulphation of oestrogens represents an important regulatory mechanism for these biologically active compounds. We have characterized and purified a form of rat liver sulphotransferase (ST), existing as a 32,500 Da monomer, which sulphates oestrogens, and have used this preparation to produce antibodies against oestrogen ST. The enzyme was active against oestrone, oestriol and beta-oestradiol, but not towards androgens. Using the antibody as a probe for immunoblotting, it was determined that the enzyme is expressed solely in male rats, and predominantly in the liver. Of the tissues examined, the only major extrahepatic tissue found to have any oestrogen ST was the brain (although the levels were very low), indicating that there might be a role for the sulphation of oestrogens in the brain. Examination of human liver and platelet cytosols by immunoblotting showed that the antibody recognized two major proteins of 32 and 34 kDa, which were presumed to correspond to the two principal phenol ST isoenzymes present in man.
authors.affiliation: Department of Biochemical Medicine, University of Dundee, Ninewells Hospital and Medical School, Scotland, U.K.
authors.forename: E B
authors.initials: EB
authors.lastname: Borthwick
authors.forename: A
authors.initials: A
authors.lastname: Burchell
authors.forename: M W
authors.initials: MW
authors.lastname: Coughtrie
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology / Q000494:pharmacology; D003600:Cytosol / Q000201:enzymology; D004970:Estrone / Q000378:metabolism*; D005260:Female; D006801:Humans; D008099:Liver / Q000201:enzymology*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D017208:Rats, Wistar; D012727:Sex Characteristics; D013379:Substrate Specificity; D015238:Sulfotransferases; D013466:Sulfurtransferases / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D014018:Tissue Distribution
chemical_list: D000906:Antibodies; D004970:Estrone; D013466:Sulfurtransferases; D015238:Sulfotransferases; C020122:estrone sulfotransferase