Purification of methylguanidine synthase from the rat kidney.
Methylguanidine (MG)-synthesizing enzyme was purified from rat kidney lysosomes and peroxisomes. The enzyme was a flavoprotein with a molecular weight of about 37,000 and oxidized creatol to produce MG. The present results suggest that the reaction mechanism of this enzyme is different from that of L-gulono-gamma-lactone oxidase (EC 1.1.3.8) isolated from rat liver microsomes.
Yokozawa
T
T
Research Institute for Wakan-Yaku, Toyama Medical and Pharmaceutical University, Sugitani, Japan.
Fujitsuka
N
N
Oura
H
H
Akao
T
T
Kobashi
K
K
Ienaga
K
K
Nakamura
K
K
Hattori
M
M
D000595:Amino Acid Sequence; D000818:Animals; D003404:Creatinine / Q000031:analogs & derivatives / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007668:Kidney / Q000201:enzymology*; D050544:L-Gulonolactone Oxidase; D008247:Lysosomes / Q000201:enzymology; D008297:Male; D008760:Methylguanidine / Q000378:metabolism; D008830:Microbodies / Q000201:enzymology; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010101:Oxygen Consumption; D010105:Oxygenases / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013401:Sugar Alcohol Dehydrogenases / Q000737:chemistry
C064402:creatol; D008760:Methylguanidine; D003404:Creatinine; D013401:Sugar Alcohol Dehydrogenases; D050544:L-Gulonolactone Oxidase; D010105:Oxygenases; C080451:methylguanidine synthase