Aromatase activity in human osteoblast-like osteosarcoma cell.
Aromatase activity was studied in cultured human osteosarcoma cell (HOS). HOS was incubated from 12 to 72 hours with 10(-10) M-10(-5) M dexamethasone. Aromatase activity was determined by measuring [3H]H2O released upon the conversion of [1 beta-3H]androstenedione to estrone. HOS showed aromatase activity, and apparent km for [1 beta-3H]androstenedione was 4.46 +/- 0.98 nm (mean +/- SD). The aromatase activity was significantly increased by 10(-9) M-10(-5) M dexamethasone in a dose-dependent manner. Dexamethasone increased Vmax of aromatase activity but did not change its km value. These results suggest that osteoblastic cells have aromatase activity which is regulated by glucocorticoid, and directly convert androgen to estrogen in itself.
Tanaka
S
S
Third Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Haji
M
M
Nishi
Y
Y
Yanase
T
T
Takayanagi
R
R
Nawata
H
H
D000735:Androstenedione / Q000378:metabolism; D001141:Aromatase / Q000032:analysis*; D003907:Dexamethasone / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004970:Estrone / Q000096:biosynthesis; D006801:Humans; D012516:Osteosarcoma / Q000201:enzymology*; D014407:Tumor Cells, Cultured / Q000201:enzymology
D004970:Estrone; D000735:Androstenedione; D003907:Dexamethasone; D001141:Aromatase