Metabolism of tetralin (1,2,3,4-tetrahydronaphthalene) in Corynebacterium sp. strain C125.
Corynebacterium sp. strain C125, originally isolated on o-xylene, was selected for its ability to grow on tetralin (1,2,3,4-tetrahydronaphthalene) as the sole source of carbon and energy. The catabolism of tetralin in Corynebacterium sp. strain C125 was shown to proceed via initial hydroxylation of the benzene nucleus at positions C-5 and C-6, resulting in the formation of the corresponding cis-dihydro diol. Subsequently, the dihydro diol was dehydrogenated by a NAD-dependent dehydrogenase to 5,6,7,8-tetrahydro-1,2-naphthalene diol. The aromatic ring was cleaved in the extradiol position by a catechol-2,3-dioxygenase. The ring fission product was subject to a hydrolytic attack, resulting in the formation of a carboxylic acid-substituted cyclohexanone. This is the first report of the catabolism of tetralin via degradation of the aromatic moiety.
Sikkema
J
J
Department of Food Science, Wageningen Agricultural University, The Netherlands.
de Bont
J A
JA
D003352:Corynebacterium / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D003512:Cyclohexanones / Q000378:metabolism*; D010101:Oxygen Consumption; D010105:Oxygenases / Q000378:metabolism*; D013764:Tetrahydronaphthalenes / Q000378:metabolism*
D003512:Cyclohexanones; D013764:Tetrahydronaphthalenes; D010105:Oxygenases