title: Quantification of pertussis toxin, filamentous haemagglutinin, 69 kDa outer membrane protein, agglutinogens 2 and 3 and lipopolysaccharide in the Danish whole-cell pertussis vaccine.
abstract: The amounts of pertussis toxin (PT), filamentous haemagglutinin (FHA), 69 kDa outer membrane protein (69 kDa OMP) and agglutinogens (AGG) 2 and 3 in extracts from the Danish whole-cell pertussis vaccine were studied in quantitative capture ELISA. With the exception of PT, the most effective extraction of these antigens was by heating the bacteria at 60 degrees C for 30 min in 2 M urea followed by sonication for 45 s. Extraction by 1 M sodium chloride prior to sonication resulted in higher levels of antigenic and biologically active PT. On average, a single human dose of pertussis vaccine (approximately 16 opacity units) was found to contain 5520 ng FHA, 63 ng PT, 1061 ng 69 kDa OMP, 397 ng AGG 2, 534 ng AGG 3 and 4840 ng lipopolysaccharide (LPS). The antigen content of one dose of the Danish pertussis vaccine appears to be low compared with the amounts found in the acellular vaccines currently in use. These findings may have important implications for the evaluation of the protective substances and the immunogenicity of whole-cell as opposed to acellular pertussis vaccines.
authors.affiliation: Bacterial Vaccine Department, Statens Seruminstitut, Copenhagen, Denmark.
authors.forename: P H
authors.initials: PH
authors.lastname: Ibsen
authors.forename: J W
authors.initials: JW
authors.lastname: Petersen
authors.forename: I
authors.initials: I
authors.lastname: Heron
mesh_terms: D018829:Adhesins, Bacterial; D000373:Agglutinins / Q000032:analysis; D000942:Antigens, Bacterial / Q000032:analysis; D001425:Bacterial Outer Membrane Proteins / Q000032:analysis; D001886:Bordetella pertussis / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D006388:Hemagglutinins / Q000032:analysis; D008070:Lipopolysaccharides / Q000032:analysis; D037342:Pertussis Toxin; D010567:Pertussis Vaccine / Q000737:chemistry* / Q000276:immunology; D010566:Virulence Factors, Bordetella / Q000032:analysis
chemical_list: D018829:Adhesins, Bacterial; D000373:Agglutinins; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D006388:Hemagglutinins; D008070:Lipopolysaccharides; D010567:Pertussis Vaccine; D010566:Virulence Factors, Bordetella; C065273:filamentous hemagglutinin adhesin, Bordetella pertussis; C068929:pertactin; D037342:Pertussis Toxin