Synthesis of tetrapeptide p-nitroanilides catalyzed by pepsin.
Swine pepsin at pH 5 efficiently catalyzes a condensation between Z-Ala-Ala-Phe-OH and p-nitroanilides of Leu, Phe, Val, Ala and Arg that leads to formation of corresponding benzyloxycarbonyl-tetrapeptide p-nitroanilides with yields of 70-90%. These reactions are complicated by co-precipitation of pepsin and the reaction products that necessitates the use of a relatively high concentration of pepsin.
Abdel Malak
C A
CA
Chemistry Department, M.V. Lomonosov Moscow State University, Russia.
Lavrenova
G I
GI
Lysogorskaya
E N
EN
Filippova IYu
Terent'eva EYu
Stepanov
V M
VM
D000595:Amino Acid Sequence; D000813:Anilides / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D009574:Nitro Compounds / Q000737:chemistry / Q000378:metabolism; D009842:Oligopeptides / Q000096:biosynthesis* / Q000737:chemistry; D010434:Pepsin A / Q000378:metabolism*; D013552:Swine
D000813:Anilides; D009574:Nitro Compounds; D009842:Oligopeptides; D010434:Pepsin A