A simple method for isolation of DNA from blood clots suited for use in PCR.
We describe a rapid, simple and inexpensive method for the isolation of DNA from blood clots suited for use in PCR. Our method is based on the lysing and nuclease-inactivating properties of guanidine thiocyanate together with the nucleic acid-binding properties of silica particles. Isolated DNA can be used for in vitro amplification as shown for a retinoblastoma gene PCR system.
Zeillinger
R
R
First Department of Obstetrics & Gynecology, University of Vienna, Austria.
Schneeberger
C
C
Speiser
P
P
Kury
F
F
D001483:Base Sequence; D001777:Blood Coagulation; D004247:DNA / Q000097:blood*; D015245:Deoxyribonuclease BamHI; D004587:Electrophoresis, Agar Gel; D016161:Genes, Retinoblastoma; D006146:Guanidines; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D013861:Thiocyanates
D006146:Guanidines; D013861:Thiocyanates; C054436:guanidine thiocyanate; D004247:DNA; D015245:Deoxyribonuclease BamHI