title: Cross-linking of surface IgM, but not surface IgD receptors, by soluble monoclonal antibodies primes murine B cells to secrete immunoglobulin in response to lymphokines.
abstract: We have previously reported the development of a two-step culture system in which soluble anti-mu monoclonal antibodies prime small resting murine B cells to secrete immunoglobulin (Ig) in response to restimulation with a mixture of interleukin-4 (IL-4) and IL-5. Here we have extended these studies to investigate the effects of engaging surface IgD (sIgD). We find that, unlike anti-mu, three different anti-delta monoclonal antibodies did not prime B cells to secrete Ig. In addition, these anti-delta antibodies inhibited anti-mu-stimulated priming for Ig secretion, while enhancing DNA synthesis in response to anti-mu. Furthermore, anti-delta antibodies still inhibited anti-mu-induced priming when added 24-48 h after anti-mu. These results therefore suggest that triggering of sIgD on B cells induces a dominant inhibitory signal which is not necessarily dependent upon co-ligation of sIgM and sIgD receptors. In addition, these findings raise the possibility that ligating sIgM or sIgD receptors on mature B cells in the absence of T cell help, may produce different downstream effects.
authors.affiliation: National Institute for Medical Research, Mill Hill, London, GB.
authors.forename: C
authors.initials: C
authors.lastname: Phillips
authors.forename: G G
authors.initials: GG
authors.lastname: Klaus
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000954:Antigens, Surface / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology*; D002478:Cells, Cultured; D004261:DNA Replication; D007138:Immunoglobulin delta-Chains / Q000276:immunology; D007148:Immunoglobulin mu-Chains / Q000276:immunology; D007136:Immunoglobulins / Q000096:biosynthesis*; D008213:Lymphocyte Activation / Q000276:immunology; D008222:Lymphokines / Q000276:immunology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D011961:Receptors, Fc / Q000276:immunology*; D011971:Receptors, Immunologic / Q000276:immunology*; D012995:Solubility
chemical_list: D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D007138:Immunoglobulin delta-Chains; D007148:Immunoglobulin mu-Chains; D007136:Immunoglobulins; D008222:Lymphokines; D011961:Receptors, Fc; D011971:Receptors, Immunologic; C050855:immunoglobulin D receptor; C035311:immunoglobulin M receptor