Immunoelectron microscopic detection of estrogen target cells in the bone marrow of estrogen-treated male Japanese quail.
The localization of estrogen receptors (ERs) in bone marrow cells was studied immunoelectron microscopically in the femurs of estrogen-treated male Japanese quail. The electron-dense reaction product showing ER localization was not detected in the bone marrow cells of the male quail. After 48 h of estrogen treatment, nuclear reaction product was observed in many mononuclear cells in the bone marrow. Most were located in the marrow stroma, and they were divided into undifferentiated and differentiated type cells, characterized by poorly and well-developed cytoplasm, respectively. Some ER-positive cells were situated in the hematopoietic tissue and had moderately developed cytoplasm. Thus, estrogen target cells may be present in the stroma and hematopoietic tissue of bone marrow.
Ohashi
T
T
Graduate School of Science and Technology, Niigata University, Japan.
Kusuhara
S
S
D000818:Animals; D001853:Bone Marrow / Q000187:drug effects / Q000378:metabolism*; D001854:Bone Marrow Cells; D002467:Cell Nucleus / Q000378:metabolism; D003370:Coturnix; D004958:Estradiol / Q000031:analogs & derivatives / Q000494:pharmacology; D008297:Male; D016253:Microscopy, Immunoelectron; D011960:Receptors, Estrogen / Q000187:drug effects / Q000378:metabolism*
D011960:Receptors, Estrogen; D004958:Estradiol