title: Mixed chimerism after sex-mismatched allogeneic BMT: evaluation of two molecular techniques.
abstract: Two different molecular techniques were used to monitor chimerism following 17 non-T cell-depleted BMTs from female donors to male recipients: pHY10, a Y chromosome-specific probe (Southern or slot blots), and a set of primers for Y chromosome sequence-specific amplification by the polymerase chain reaction (PCR). On Southern blots, male DNA was detectable at a level less than 1% of 10 micrograms DNA while cross-reactivity with autosomal sequences was avoided. On slot blots, male DNA was reliably detectable at levels less than 0.5%, even in small sample (0.5 microgram DNA). With the PCR technique, male DNA was detectable at levels of 1:10(6) to 1:10(7) of 0.5 microgram DNA. Slot blot and PCR results were concordant in 19 of 23 samples. Both techniques demonstrated a constant small mixed chimerism during the first year after BMT and in four of nine patients, this chimerism persisted even longer (up to 29 months after BMT).
authors.affiliation: Laboratoire d'Hématologie Université de Bordeaux II, France.
authors.forename: F
authors.initials: F
authors.lastname: Viard
authors.forename: P
authors.initials: P
authors.lastname: Merel
authors.forename: C
authors.initials: C
authors.lastname: Bilhou-Nabera
authors.forename: G
authors.initials: G
authors.lastname: Marit
authors.forename: F
authors.initials: F
authors.lastname: Comeau
authors.forename: M J
authors.initials: MJ
authors.lastname: Gharbi
authors.forename: F
authors.initials: F
authors.lastname: Febrer
authors.forename: F
authors.initials: F
authors.lastname: Belloc
authors.forename: F
authors.initials: F
authors.lastname: Lacombe
authors.forename: A
authors.initials: A
authors.lastname: Broustet
mesh_terms: D000293:Adolescent; D000328:Adult; D015139:Blotting, Southern; D016026:Bone Marrow Transplantation / Q000473:pathology*; D002648:Child; D002675:Child, Preschool; D002678:Chimera / Q000235:genetics*; D005069:Evaluation Studies as Topic; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D015336:Molecular Probe Techniques / Q000706:statistics & numerical data; D016133:Polymerase Chain Reaction / Q000379:methods / Q000706:statistics & numerical data; D012680:Sensitivity and Specificity; D012723:Sex; D014019:Tissue Donors; D014998:Y Chromosome