title: Polymerase chain reaction approaches to Culicoides (Diptera: Ceratopogonidae) identification.
abstract: Molecular genetic approaches such as polymerase chain amplification (PCR) of target genomic sequences are finding wide application in systematic and taxonomic studies of arthropods. PCR-based techniques that preclude the need for target DNA sequence information of the species of interest facilitate molecular taxonomic studies. Two such techniques, tDNA-PCR (DNA encoding tRNAs is the analyte) and RAPD-PCR (randomly amplified polymorphic DNA) were investigated for their ability to differentiate certain North American Culicoides spp. larvae and adults.
authors.affiliation: Department of Microbiology, Colorado State University, Fort Collins 80523.
authors.forename: T J
authors.initials: TJ
authors.lastname: Raich
authors.forename: J L
authors.initials: JL
authors.lastname: Archer
authors.forename: M A
authors.initials: MA
authors.lastname: Robertson
authors.forename: W J
authors.initials: WJ
authors.lastname: Tabachnick
authors.forename: B J
authors.initials: BJ
authors.lastname: Beaty
mesh_terms: D000818:Animals; D001483:Base Sequence; D002519:Ceratopogonidae / Q000145:classification* / Q000235:genetics; D004277:DNA, Single-Stranded / Q000737:chemistry; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012343:RNA, Transfer / Q000032:analysis*
chemical_list: D004277:DNA, Single-Stranded; D012343:RNA, Transfer