111Indium-F(ab)'2-NCA 102 monoclonal antibody: in vitro study of a specific agent for the detection of inflammatory foci.
A new antigranulocyte antibody was evaluated in vitro for the detection of inflammatory foci in man. The specificity for polymorphonuclear cells (PMN) of NCA 102, an anti-NCA 95 monoclonal IgG1, was determined with immunohistochemical and cytofluorometrical tests. Its affinity, assessed by Scatchard analysis, was 1.1 x 10(9) L/mol and the number of epitopes per granulocyte reached about 10(5). The biological properties of PMNs incubated with NCA 102 were not inhibited even when coupled with DTPA. A F(ab)'2 fragment was radiolabelled with 111Indium and incubated in the presence of whole blood. More than 65% radioactivity was selectively taken up by the PMN population. These findings indicated that NCA 102 antibody is suitable for sepsis detection.
Collet
B
B
Centre Régional de Lutte contre le Cancer, Rennes, France.
Maros
S
S
Moisan
A
A
Le Cloirec
J
J
Moinereau
M
M
Aumaitre
E
E
Toujas
L
L
Bourguet
P
P
D000818:Animals; D000911:Antibodies, Monoclonal; D001769:Blood / Q000000981:diagnostic imaging; D001853:Bone Marrow / Q000648:ultrastructure; D006098:Granulocytes / Q000378:metabolism; D006801:Humans; D007205:Indium Radioisotopes; D007249:Inflammation / Q000000981:diagnostic imaging*; D008099:Liver / Q000648:ultrastructure; D051379:Mice; D008807:Mice, Inbred BALB C; D011877:Radionuclide Imaging
D000911:Antibodies, Monoclonal; D007205:Indium Radioisotopes