Increasing DNase I activity after exposure of isolated DNA to halothane.
DNA was exposed to halothane (CAS 151-67-7) in a cell-free system. After exposure the DNA was used as substrate for DNase I from bovine pancreas. The DNase I activity increased after halothane exposure of the substrate depending on time and doses. Drugs are able to influence the DNA conformation. Conformational changes in the DNA can enhance the DNase I cleavage rate. Therefore, it is possible that halothane exposure induces changes in DNA conformation demonstrable by an increased DNase I activity. The results suggest a mechanism by which halothane may contribute to chromosomal defects and disturbances of DNA metabolism in cells.
Reitz
M
M
Department of Physiological Chemistry, University of Mainz, Fed. Rep. of Germany.
Knitza
R
R
Lanz
E
E
D000818:Animals; D002417:Cattle; D002875:Chromosomes / Q000187:drug effects; D004247:DNA / Q000187:drug effects*; D004260:DNA Repair / Q000187:drug effects; D003850:Deoxyribonuclease I / Q000378:metabolism*; D006221:Halothane / Q000494:pharmacology*; D009690:Nucleic Acid Conformation / Q000187:drug effects; D010179:Pancreas / Q000201:enzymology; D012616:Sea Cucumbers / Q000502:physiology*
D004247:DNA; D003850:Deoxyribonuclease I; D006221:Halothane