KatF (sigma S) synthesis in Escherichia coli is subject to posttranscriptional regulation.
A transcriptional fusion of katF to the lacZ gene was expressed at increasingly higher levels throughout the exponential phase, but a translational fusion was expressed at low levels during exponential-phase growth and was induced 160-fold during the transition to stationary phase, implicating a posttranscriptional mechanism in the regulation of KatF synthesis. Mutational analyses suggested that the initiation codon of katF is the second ATG in the previously identified open reading frame.
Loewen
P C
PC
Department of Microbiology, University of Manitoba, Winnipeg, Canada.
von Ossowski
I
I
Switala
J
J
Mulvey
M R
MR
D001426:Bacterial Proteins / Q000235:genetics*; D010582:Bacteriophage lambda / Q000235:genetics; D001483:Base Sequence; D003062:Codon; D004252:DNA Mutational Analysis; D004926:Escherichia coli / Q000235:genetics* / Q000254:growth & development; D015964:Gene Expression Regulation, Bacterial; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics; D014176:Protein Biosynthesis; D012323:RNA Processing, Post-Transcriptional; D011994:Recombinant Proteins / Q000096:biosynthesis; D012808:Sigma Factor / Q000235:genetics*; D014158:Transcription, Genetic; D001616:beta-Galactosidase / Q000096:biosynthesis / Q000235:genetics
D001426:Bacterial Proteins; D003062:Codon; D011994:Recombinant Proteins; D012808:Sigma Factor; C069567:sigma factor KatF protein, Bacteria; D001616:beta-Galactosidase