Fimbriated stationary phases for proteins.
This paper describes synthetic procedures for preparing fimbriated stationary phases on poly(styrene-divinylbenzene) (PS-DVB) packing materials. The synthesis consists of a five-step procedure in which the order in which the steps are carried out may be varied. These steps are (i) polymerization of monomers to form an amphiphilic copolymer or oligomer, (ii) adsorption of either monomers, or polymer onto the PS-DVB surface, (iii) solvent induction of functional group orientation at the PS-DVB-polymer interface and polymer-water interface, (iv) a cross-linking reaction that forms a hydrophilic surface layer, and (v) derivatization of the surface layer with stationary phase.
Varady
L
L
PerSeptive Biosystems, Cambridge, MA 02139.
Mu
N
N
Yang
Y B
YB
Cook
S E
SE
Afeyan
N
N
Regnier
F E
FE
D002846:Chromatography, Affinity / Q000295:instrumentation*; D002852:Chromatography, Ion Exchange / Q000295:instrumentation*; D005990:Glycerol / Q000737:chemistry; D007475:Ion Exchange Resins; D008689:Methacrylates / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D011137:Polystyrenes; D011506:Proteins / Q000302:isolation & purification*; D013056:Spectrophotometry, Ultraviolet
D007475:Ion Exchange Resins; D008689:Methacrylates; D011108:Polymers; D011137:Polystyrenes; D011506:Proteins; C043941:polyglycerol; C003771:Amberlite XAD-2 resin; D005990:Glycerol