title: Evidence for an increase in the association of cytosolic phospholipase A2 with the cytoskeleton of stimulated rabbit platelets.
abstract: Following stimulation of rabbit platelets with thrombin, phospholipase A2 (PLA2) activity increased in the Triton X-100-insoluble residue. Although the PLA2 activity was dependent on the protein content of the residue from the stimulated cells, the specific activity was higher than that in the case of unstimulated cells. The enzyme activity was inhibited by p-bromophenacyl bromide and increased significantly with 0.5-10 microM Ca2+. The enzyme hydrolyzed phospholipids having an arachidonoyl residue more effectively than ones with a linoleoyl residue. In addition, 70% of the enzyme activity was immunoprecipitated with a monoclonal antibody against cytosolic PLA2 of rabbit platelets, while it was inhibited by only 20% by an antibody that neutralizes the activity of group II PLA2. These results suggest an increase in the association of cytosolic PLA2 with cytoskeleton upon stimulation of rabbit platelets.
authors.affiliation: Department of Biochemistry, Kyoto Pharmaceutical University.
authors.forename: S
authors.initials: S
authors.lastname: Akiba
authors.forename: T
authors.initials: T
authors.lastname: Sato
authors.forename: T
authors.initials: T
authors.lastname: Fujii
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D001792:Blood Platelets / Q000201:enzymology*; D003599:Cytoskeleton / Q000201:enzymology*; D003600:Cytosol / Q000201:enzymology*; D006868:Hydrolysis; D010741:Phospholipases A / Q000378:metabolism*; D054467:Phospholipases A2; D010743:Phospholipids / Q000378:metabolism; D011817:Rabbits; D013379:Substrate Specificity; D013917:Thrombin / Q000494:pharmacology
chemical_list: D000911:Antibodies, Monoclonal; D010743:Phospholipids; D010741:Phospholipases A; D054467:Phospholipases A2; D013917:Thrombin