A simple G-banding technique following nonradioactive in situ hybridization.
A simple G-banding protocol has been found to be compatible with a nonisotopic in situ hybridization technique utilizing a biotin-streptavidin-polyalkaline phosphatase complex. The chromosomes are banded following detection of the hybridization signal, the procedure requires no pretreatment of cultures or special optical equipment, and it produces permanent preparations.
Duncan
L M
LM
Department of Medicine, University of Melbourne, Victoria, Australia.
Dietzsch
E
E
Rudduck
C
C
Garson
O M
OM
D002871:Chromosome Banding; D006801:Humans; D017403:In Situ Hybridization; D007621:Karyotyping; D014407:Tumor Cells, Cultured