Alteration of superhelical state of DNA by aluminium (Al).
The effect of aluminium (Al) on the supercoiled state of pUC18 DNA was studied by ethidium bromide fluorescence and agarose gel electrophoresis. Al at physiologically relevant concentrations relaxed the intact supercoiled DNA as well as the topoisomers induced by chloroquine. EDTA prevented the unwinding effect of Al on supercoiled DNA. Al did not alter the mobility of linear DNA in agarose gels. The implications of this finding in neurological disorders are discussed.
Rao
K S
KS
Nutrition and Food Safety Unit, Central Food Technological Research Institute (C.F.T.R.I.), Mysore, India.
Rao
B S
BS
Vishnuvardhan
D
D
Prasad
K V
KV
D000535:Aluminum / Q000494:pharmacology*; D010582:Bacteriophage lambda; D002738:Chloroquine / Q000494:pharmacology; D004278:DNA, Superhelical / Q000737:chemistry* / Q000187:drug effects; D004279:DNA, Viral / Q000737:chemistry / Q000187:drug effects; D015247:Deoxyribonuclease HindIII; D004587:Electrophoresis, Agar Gel; D004996:Ethidium / Q000494:pharmacology; D007700:Kinetics; D009690:Nucleic Acid Conformation / Q000187:drug effects*; D010957:Plasmids; D013050:Spectrometry, Fluorescence
D004278:DNA, Superhelical; D004279:DNA, Viral; D002738:Chloroquine; D000535:Aluminum; D015247:Deoxyribonuclease HindIII; D004996:Ethidium