Association of erythrocyte aldose reductase activity with diabetic complications in type 1 diabetes mellitus.
Erythrocyte aldose reductase was isolated and its activity measured in 72 Type 1 (insulin dependent) diabetic patients and 21 age and sex matched non-diabetic subjects. The diabetic patients were categorized into two groups in terms of presence (n = 29) or absence (n = 43) of severe diabetic complications. Age, sex, duration of diabetes and HbA1c levels were matched between the diabetic groups. Erythrocyte aldose reductase (mean +/- SEM) was increased in patients with Type 1 diabetes compared to the non-diabetic subjects (7.22 +/- 0.24 vs 5.66 +/- 0.19 Ul-erythrocytes-1, < 0.0001). There was a four-fold variation in its activity among the diabetic patients (3.38-12.23 Ul-erythrocytes-1). The enzyme activity was significantly higher in patients with complications than those without (8.17 +/- 0.39 vs 6.58 +/- 0.26 Ul-erythrocytes-1, p < 0.002). When the patients were stratified by duration of the disease, the enzyme activity was highest in patients who had developed complications with a duration of less than 20 years and lowest in those without complications for 20 years or longer (8.54 +/- 0.48 vs 6.46 +/- p +/- 0.33 Ul-erythrocytes-1, p < 0.002). Patients who had an aldose reductase activity greater than the mean +/- 2SD of that seen in non-diabetic controls were four times more likely to have diabetic complications than those whose enzyme activity fell within 2SD of non-diabetic individuals (p < 0.0005).(ABSTRACT TRUNCATED AT 250 WORDS)
Hamada
Y
Y
Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas 75235-8858.
Kitoh
R
R
Raskin
P
P
D000328:Adult; D000449:Aldehyde Reductase / Q000097:blood* / Q000302:isolation & purification; D015415:Biomarkers / Q000097:blood; D003922:Diabetes Mellitus, Type 1 / Q000097:blood / Q000201:enzymology*; D003928:Diabetic Nephropathies / Q000097:blood / Q000201:enzymology* / Q000453:epidemiology; D003929:Diabetic Neuropathies / Q000097:blood / Q000201:enzymology* / Q000453:epidemiology; D003930:Diabetic Retinopathy / Q000097:blood / Q000201:enzymology* / Q000453:epidemiology; D004912:Erythrocytes / Q000201:enzymology*; D005260:Female; D006442:Glycated Hemoglobin / Q000032:analysis; D006801:Humans; D008297:Male; D015995:Prevalence; D012016:Reference Values
D000449:Aldehyde Reductase; D015415:Biomarkers; D006442:Glycated Hemoglobin