Lovastatin potentiates the photocytotoxic effect of photofrin II delivered to HT29 human colonic adenocarcinoma cells by low density lipoprotein.
A 24 h preculture of HT29-18 human colonic adenocarcinoma cells with the sterol synthesis inhibitor lovastatin at concentrations of 0.1-0.5 microM markedly increased the photocytotoxic effect of photofrin II delivered to cells by low density lipoproteins. Under the same conditions, LDL binding and photofrin II (PII) uptake by HT29 cells increased about 1.8-fold and 1.5-fold, respectively. These results suggest that hydroxymethylglutaryl-coenzyme A reductase inhibitors could be useful for potentiating the photodynamic therapy of tumors by PII.
Biade
S
S
Laboratoire de Physico-Chimie de l'Adaptation Biologique, INSERM U312, Muséum National d'Histoire Naturelle de Paris, France.
Mazière
J C
JC
Mora
L
L
Santus
R
R
Mazière
C
C
Auclair
M
M
Morlière
P
P
Dubertret
L
L
D000230:Adenocarcinoma; D001692:Biological Transport; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D003110:Colonic Neoplasms; D017323:Dihematoporphyrin Ether / Q000008:administration & dosage / Q000378:metabolism / Q000494:pharmacology*; D004357:Drug Synergism; D006801:Humans; D008027:Light; D008077:Lipoproteins, LDL / Q000378:metabolism*; D008148:Lovastatin / Q000494:pharmacology*; D011973:Receptors, LDL / Q000187:drug effects / Q000378:metabolism; D013261:Sterols / Q000096:biosynthesis; D014407:Tumor Cells, Cultured
D008077:Lipoproteins, LDL; D011973:Receptors, LDL; D013261:Sterols; D017323:Dihematoporphyrin Ether; D008148:Lovastatin