Crystallization and preliminary X-ray analysis of a truncated tissue metalloproteinase inhibitor delta 128-194 TIMP-2.
Crystals of an active truncated form of a human tissue metallproteinase inhibitor (delta 128-194 TIMP-2), a protein implicated in a number of diseases of the connective tissues, have been grown in a form suitable for study by X-ray diffraction analysis. Crystals grow using polyethylene glycol 600 as a precipitant and are in space group P2(1)2(1)2, with cell dimensions a = 75.28 A, b = 100.77 A, c = 31.45 A. The asymmetric unit appears to contain two molecules of delta TIMP-2 with a Vm value of 2.3 A3/dalton. The crystals diffract to Bragg-spacings beyond 2.5 A. Native data to 3.2 A have been collected and a search for heavy-metal derivatives is in progress.
Tolley
S
S
Department of Chemistry, University of York, Heslington, U.K.
Murphy
G
G
O'Shea
M
M
Ward
R
R
Docherty
A
A
Cockett
M
M
Rawas
A
A
Davies
G
G
D003460:Crystallization; D006801:Humans; D008666:Metalloendopeptidases / Q000737:chemistry*; D009363:Neoplasm Proteins / Q000737:chemistry*; D010446:Peptide Fragments / Q000737:chemistry; D019716:Tissue Inhibitor of Metalloproteinase-2; D014961:X-Ray Diffraction
D009363:Neoplasm Proteins; D010446:Peptide Fragments; D019716:Tissue Inhibitor of Metalloproteinase-2; D008666:Metalloendopeptidases