title: Direct demonstration of membrane IL-1 alpha on the surface of circulating B lymphocytes and monocytes.
abstract: A mAb against IL-1 alpha has been used in a high sensitivity immunofluorescence procedure to demonstrate directly the presence of surface IL-1 alpha on human monocytes and B cells from blood and tonsil. IL-1 alpha could be detected on monocytes and a subset (around 30%) of B cells without any in vitro activation. Although the subset of B cells expressing IL-1 alpha was distinct (i.e., the distribution of fluorescence intensity was bimodal), no correlation was seen with any other B cell subset marker in blood. In tonsil, IL-1 alpha was expressed by cells with a phenotype that suggested that they represented a subset of mantle zone B cells. Activation of tonsil B cells with anti-IgM, IL-4, IL-2, and mixtures of these reagents led to small increases in IL-1 alpha expression, as did activation of unfractionated tonsil cells with PHA.
authors.affiliation: Department of Clinical Immunology, Flinders Medical Centre, Adelaide, South Australia.
authors.forename: H
authors.initials: H
authors.lastname: Zola
authors.forename: L
authors.initials: L
authors.lastname: Flego
authors.forename: Y T
authors.initials: YT
authors.lastname: Wong
authors.forename: P J
authors.initials: PJ
authors.lastname: Macardle
authors.forename: J S
authors.initials: JS
authors.lastname: Kenney
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000032:analysis; D016175:B-Lymphocyte Subsets / Q000737:chemistry / Q000276:immunology; D001402:B-Lymphocytes / Q000737:chemistry*; D002462:Cell Membrane / Q000737:chemistry; D006801:Humans; D007375:Interleukin-1 / Q000032:analysis* / Q000097:blood; D007938:Leukemia / Q000378:metabolism; D008213:Lymphocyte Activation; D051379:Mice; D009000:Monocytes / Q000737:chemistry*; D014066:Palatine Tonsil / Q000737:chemistry / Q000166:cytology
chemical_list: D015703:Antigens, CD; D007375:Interleukin-1