Chronic sabeluzole treatment of cultured rat cerebellar granule cells reduces N-methyl-D-aspartate-induced inward current.
The effects of sabeluzole, a drug that protects rat hippocampal neurones from glutamate- and N-methyl-D-aspartate (NMDA)-induced toxicity, were investigated in rat cerebellar granule cells in vitro with the whole-cell voltage clamp technique. Acute exposure of 0.1 microM sabeluzole for 20 min prior to experiments did not significantly affect glutamate receptor-mediated inward currents. Conversely, exposure of cultured granule cells to sabeluzole for 7 days reduced the NMDA-induced inward current and did not affect the non-NMDA responses evoked by kainic acid. The results suggest that chronic treatment with sabeluzole selectively reduces the functional NMDA response.
Van der Valk
J B
JB
Research Institute of Toxicology, Utrecht University, Netherlands.
Vijverberg
H P
HP
D000818:Animals; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology / Q000187:drug effects*; D008564:Membrane Potentials / Q000187:drug effects; D016202:N-Methylaspartate / Q000037:antagonists & inhibitors*; D010880:Piperidines / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D017470:Receptors, Glutamate / Q000187:drug effects; D016194:Receptors, N-Methyl-D-Aspartate / Q000187:drug effects; D013844:Thiazoles / Q000494:pharmacology*
D010880:Piperidines; D017470:Receptors, Glutamate; D016194:Receptors, N-Methyl-D-Aspartate; D013844:Thiazoles; D016202:N-Methylaspartate; C055163:sabeluzole