Time-resolved immunofluorometric assay of complement C3: application to cerebrospinal fluid.
Complement components have a role in various neurological disorders. Complement C3 can be measured by immunochemical methods, but only radioimmunoassays and electroimmunodiffusion assays (EIDs) are sufficiently sensitive to be applied to biological fluids in which the C3 concentration is low, especially cerebrospinal fluid (CSF). We report a sandwich-type time-resolved immunofluorometric assay (TR-IFMA) for C3 in CSF. The linearity (0.7-3650 micrograms/L) and intra- (CV < 4.8%) and inter-assay (CV < 10.9%) precision were satisfactory and the results agreed with those of EID. The assay is extremely sensitive (< 1 microgram/L) and its analytical range is large and well suited to clinical applications. This simple TR-IFMA is thus a nonisotopic alternative to radioimmunoassay for the quantification of complement C3 in CSF.
Gaillard
O
O
Laboratoire d'Immunochimie, Hôpital de la Salpêtrière, Paris, France.
Meillet
D
D
Diemert
M C
MC
Musset
L
L
Delattre
J
J
Schuller
E
E
Galli
J
J
D000328:Adult; D003176:Complement C3 / Q000134:cerebrospinal fluid*; D005260:Female; D015200:Fluoroimmunoassay / Q000379:methods* / Q000592:standards / Q000706:statistics & numerical data; D015658:HIV Infections / Q000134:cerebrospinal fluid; D006801:Humans; D008297:Male; D008832:Microchemistry; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000134:cerebrospinal fluid; D009422:Nervous System Diseases / Q000134:cerebrospinal fluid; D012016:Reference Values
D003176:Complement C3