Rapid and specific identification of Yersinia pestis by using a nested polymerase chain reaction procedure.
We developed a 4-h nested polymerase chain reaction assay that detected a region of the plasminogen activator gene of Yersinia pestis in 100% of 43 Y. pestis strains isolated from humans, rats, and fleas yet was unreactive with the closely related species Yersinia enterocolitica and Yersinia pseudotuberculosis.
Campbell
J
J
Naval Research Laboratory, Washington, D.C. 20375.
Lowe
J
J
Walz
S
S
Ezzell
J
J
D001483:Base Sequence; D005798:Genes, Bacterial / Q000235:genetics*; D008969:Molecular Sequence Data; D010960:Plasminogen Activators / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D013045:Species Specificity; D015010:Yersinia pestis / Q000235:genetics / Q000302:isolation & purification*
D010960:Plasminogen Activators