Isolation and ordering of bacteriophage genomic clones corresponding to two YACs from 19q13.3.
We describe a method for rapidly isolating overlapping bacteriophage clones corresponding to the genomic region cloned in a yeast artificial chromosome (YAC) that does not require sub-cloning or lambda DNA preparation. Purified YACs from 19q13.3 were used to screen a flow-sorted chromosome 19 library, and the resulting positive clones were characterized using inter-Alu PCR. In addition, aliquots of the lambda stocks were gridded out, and hybridized with probes known to be present in the YACs, thereby avoiding having to perform DNA preparations. The application of this technique in the identification of lambda clones which span the myotonic dystrophy (DM) locus on 19q13.3 is presented, and its general advantages are discussed.
Buxton
J
J
Department of Anatomy, Charing Cross and Westminster Medical School, London, UK.
Davies
J
J
Shelbourne
P
P
Yokobata
K
K
Williamson
R
R
Johnson
K
K
D010582:Bacteriophage lambda / Q000235:genetics*; D001483:Base Sequence; D002874:Chromosome Mapping; D015825:Chromosomes, Fungal; D002888:Chromosomes, Human, Pair 19; D015723:Gene Library; D005814:Genes, Viral; D015894:Genome, Human; D006801:Humans; D008969:Molecular Sequence Data; D009223:Myotonic Dystrophy / Q000235:genetics; D016133:Polymerase Chain Reaction; D012091:Repetitive Sequences, Nucleic Acid; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D015678:Viral Structural Proteins / Q000235:genetics
D015678:Viral Structural Proteins