Two non-histone proteins are associated with the promoter region and histone HI with the transcribed region of active hsp-70 genes as revealed by UV-induced DNA-protein crosslinking in vivo.
We described here an approach for mapping proteins on any sequence of genomic DNA. UV-induced DNA-protein crosslinking within whole cells and the 'protein image' hybridization technique (1) were applied to test the proteins bound to different regions of the D. melanogaster hsp-70 gene. The histone H1-DNA association with the coding region is shown to be maintained, even during very intensive transcription, but is absent in the promoter. Two non-histone proteins with apparent molecular masses of 50 kD (p50) and 100 kD (p100) are crosslinked only to the active hsp-70 gene regulatory region and preferentially bind to its complementary and coding DNA strands, respectively.
Belikov
S V
SV
W.A. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow.
Belgovsky
A I
AI
Preobrazhenskaya
O V
OV
Karpov
V L
VL
Mirzabekov
A D
AD
D000818:Animals; D001665:Binding Sites; D002478:Cells, Cultured; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism* / Q000528:radiation effects; D004331:Drosophila melanogaster; D006360:Heat-Shock Proteins / Q000235:genetics*; D006657:Histones / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic / Q000502:physiology; D014466:Ultraviolet Rays
D002868:Chromosomal Proteins, Non-Histone; D004268:DNA-Binding Proteins; D006360:Heat-Shock Proteins; D006657:Histones