title: Hypoproteinemia slows lung liquid clearance in young lambs.
abstract: To determine whether hypoproteinemia slows the rate at which liquid is cleared from the lung lumen, we studied 36 lambs, 18 of which underwent repeated plasmapheresis, reducing plasma protein concentration by 37% and plasma protein osmotic pressure by 39%. We killed 29 lambs (14 hypoproteinemic and 15 normoproteinemic) and removed their lungs 1, 2, or 6 h after intratracheal instillation of isotonic saline (6 ml/kg body wt). We measured extravascular lung water and determined the percentage of tracheally instilled liquid that was cleared from the lungs by comparison with control lambs that did not receive saline into their airways. The percent liquid cleared from the lungs after 1 and 2 h was significantly less in hypoproteinemic than in normoproteinemic lambs (37 vs. 65% at 1 h, 58 vs. 75% at 2 h, respectively). By 6 h nearly all the liquid (> 92%) was cleared from the lungs of all lambs. Thus hypoproteinemia slows the initial rate of clearance of liquid from the lungs of lambs. To determine whether reduced plasma protein osmotic pressure might redirect this liquid into lung lymphatics, we measured lung lymph flow (Q1) in five lambs (7.7 +/- 1.4 kg, 19 +/- 4 days old) for > or = 2 h before and 6 h after tracheal instillation of saline. In each lamb, paired studies were done 3-6 days apart; between studies the lambs underwent plasmapheresis.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Cardiovascular Research Institute, University of California, San Francisco 94143.
authors.forename: J J
authors.initials: JJ
authors.lastname: Cummings
authors.forename: D P
authors.initials: DP
authors.lastname: Carlton
authors.forename: F R
authors.initials: FR
authors.lastname: Poulain
authors.forename: J U
authors.initials: JU
authors.lastname: Raj
authors.forename: R D
authors.initials: RD
authors.lastname: Bland
mesh_terms: D000818:Animals; D000831:Animals, Newborn / Q000502:physiology*; D001794:Blood Pressure / Q000502:physiology; D001798:Blood Proteins / Q000378:metabolism; D002199:Capillary Permeability; D015633:Extravascular Lung Water / Q000502:physiology*; D007019:Hypoproteinemia / Q000503:physiopathology*; D008196:Lymph / Q000502:physiology; D008208:Lymphatic System / Q000502:physiology; D010100:Oxygen / Q000097:blood; D011650:Pulmonary Alveoli / Q000503:physiopathology; D011652:Pulmonary Circulation / Q000502:physiology; D012129:Respiratory Function Tests; D012756:Sheep
chemical_list: D001798:Blood Proteins; D010100:Oxygen