Temporal and spatial patterns of expression of c-fos, zif/268, c-jun and jun-B mRNAs in rat brain following seizures evoked focally from the deep prepiriform cortex.
Using in situ hybridization, we previously investigated (17) the regional pattern of c-fos mRNA increases in the brain following convulsive seizures elicited from a highly circumscribed epileptogenic site located in the deep prepiriform cortex. In this paper, we focus on the hippocampus and examine mRNAs encoding other immediate early genes (IEGs), namely c-jun, jun-B, and zif/268, for changes following the focally evoked seizures. Although the anatomic distribution of increases in each IEG mRNA was qualitatively comparable, the temporal analysis indicated that increases in zif/268 mRNA appeared prior to the other genes studied. Each of the mRNAs reached a maximum increase by 30 min and declined to basal levels within 3 h following seizure initiation. The data indicate that these four IEGs respond in a coordinated fashion to propagated seizure activity with increases in mRNA and, furthermore, that increased expression of all four genes appears to occur in the same cell types in the hippocampus.
Lanaud
P
P
Fidia-Georgetown Institute of the Neurosciences, Washington, DC.
Maggio
R
R
Gale
K
K
Grayson
D R
DR
D000818:Animals; D015152:Blotting, Northern; D001921:Brain / Q000378:metabolism* / Q000473:pathology; D004268:DNA-Binding Proteins / Q000235:genetics*; D051766:Early Growth Response Protein 1; D017874:Immediate-Early Proteins; D017403:In Situ Hybridization; D008297:Male; D009833:Olfactory Pathways / Q000502:physiology*; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics*; D016755:Proto-Oncogene Proteins c-jun / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011930:Reaction Time; D012640:Seizures / Q000209:etiology / Q000378:metabolism* / Q000473:pathology; D014018:Tissue Distribution; D014157:Transcription Factors / Q000235:genetics*
D004268:DNA-Binding Proteins; D051766:Early Growth Response Protein 1; C486110:Egr1 protein, rat; D017874:Immediate-Early Proteins; D016760:Proto-Oncogene Proteins c-fos; D016755:Proto-Oncogene Proteins c-jun; D012333:RNA, Messenger; D014157:Transcription Factors