Modification of cell surfaces by enzymatic introduction of special sialic acid analogues.
Taking advantage of the defined acceptor specificity of rat liver alpha 2,6-sialyltransferase, N-linked Gal beta 1,4GlcNAc-sequences of glycoconjugates at the plasma membrane of viable human erythrocytes and lymphoblastic IM-9 cells were modified to terminate in three NeuAc analogues endowed with special biochemical properties: sialidase-resistant 9-amino-NeuAc, O-acetylesterase-resistant 9-acetamido-NeuAc and photolabile 9-azido-NeuAc. Incorporation of the latter analogues and of parent NeuAc proceeded to similar values, whereas 9-amino-NeuAc yielded slightly reduced values.
Kosa
R E
RE
Institut für Biochemie II, Universität Heidelberg, Germany.
Brossmer
R
R
Gross
H J
HJ
D002478:Cells, Cultured; D004910:Erythrocyte Membrane / Q000378:metabolism; D006001:Glycoconjugates / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D015394:Molecular Structure; D012794:Sialic Acids / Q000378:metabolism*; D012799:Sialyltransferases / Q000378:metabolism*
D006001:Glycoconjugates; D012794:Sialic Acids; D012799:Sialyltransferases