Ribonuclease activity of sialic acid-binding lectin from Rana catesbeiana eggs.
Sialic acid-binding lectin (SBL) isolated from Rana catesbeiana eggs is a basic protein which agglutinates a large variety of tumour cells and has an amino acid sequence homologous to that of human angiogenin and pancreatic ribonuclease (RNase). Although SBL and angiogenin lack the Cys-65-Cys-72 disulphide bond of pancreatic RNase, the locations of the other three disulphide bonds are similar among the three molecules. SBL was found to exhibit RNase activity, as well as catalytic properties resembling those of bovine RNase A in some respects. For example, SBL hydrolyses poly(uridylic acid) and poly(cytidylic acid) as substrates, and prefers the former. RNase A and angiogenin are strongly inhibited by human placental RNase inhibitor, whereas the RNase activity and tumour cell agglutination activity of SBL are not affected by this inhibitor.
Nitta
K
K
Department of Biochemistry, Tohoku College of Pharmaceutical Sciences, Sendai, Japan.
Oyama
F
F
Oyama
R
R
Sekiguchi
K
K
Kawauchi
H
H
Takayanagi
Y
Y
Hakomori
S
S
Titani
K
K
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002417:Cattle; D003488:Cyanogen Bromide; D006801:Humans; D037102:Lectins / Q000737:chemistry / Q000378:metabolism*; D008969:Molecular Sequence Data; D010063:Ovum / Q000737:chemistry*; D010446:Peptide Fragments / Q000302:isolation & purification / Q000378:metabolism; D011506:Proteins / Q000737:chemistry; D012335:RNA, Ribosomal / Q000378:metabolism; D011892:Rana catesbeiana; D012259:Ribonuclease, Pancreatic / Q000737:chemistry; D012260:Ribonucleases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D063265:Sialic Acid Binding Immunoglobulin-like Lectins; D014357:Trypsin / Q000378:metabolism
D000596:Amino Acids; D037102:Lectins; D010446:Peptide Fragments; D011506:Proteins; D012335:RNA, Ribosomal; D063265:Sialic Acid Binding Immunoglobulin-like Lectins; D012260:Ribonucleases; C046366:angiogenin; D012259:Ribonuclease, Pancreatic; D014357:Trypsin; D003488:Cyanogen Bromide