title: Analysis of the Spirochaeta aurantia flaA gene and transcript.
abstract: The flaA gene, which codes for the Spirochaeta aurantia flagellar filament outer layer polypeptide, FlaA, was cloned, sequenced and analysed. The gene appears to be transcribed into a monocistronic mRNA from a sigma 70-like promoter. The translational start is 31 base pairs after the start of the transcript, the open reading frame is 1011 base pairs, and a rho-independent-like transcription terminator sequence begins about 19 base pairs after the translational stop codon. The deduced amino acid sequence of the S. aurantia FlaA showed 40% identity with the Treponema pallidum FlaA, but these polypeptides did not show a significant similarity to other polypeptides for which sequence information was available.
authors.affiliation: Department of Microbiology, University of Iowa, Iowa City 52242.
authors.forename: J
authors.initials: J
authors.lastname: Parales
authors.forename: E P
authors.initials: EP
authors.lastname: Greenberg
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D005407:Flagella / Q000378:metabolism; D005408:Flagellin; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D013142:Spirochaeta / Q000235:genetics*; D014158:Transcription, Genetic; D014210:Treponema pallidum / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D012333:RNA, Messenger; D005408:Flagellin; C067138:flaA protein, bacteria