Titration of replication activity by increasing ARS dosage in yeast plasmids.
The rep1 region of the yeast mitochondrial genome, a putative replication origin, contains a weak autonomously replicating sequence (ARS). Nucleotide-sequence and deletion analyses have identified two 11-base pair ARS consensus sequences, numerous near matches to the ARS core, and a region of curvature that may contribute to ARS function. Based on the amplified nature of petite-derivative mitochondrial DNA encompassing this locus, we have constructed plasmids containing an increasing dosage of ARS elements. The rep1 ARS element can have an additive effect on plasmid stability when present either as a tandem dimer or as an unlinked pair. However, the presence of a third ARS copy does not further enhance plasmid stability. These results indicate that measurable dosage effects can be defined only in circumstances where weak ARS elements are employed, and that plasmid maintenance within yeast cells is saturable and varies among the different sequences promoting replication.
Hyman
B C
BC
Department of Biology, University of California, Riverside 92521.
Garcia-Garcia
F
F
D001483:Base Sequence; D003001:Cloning, Molecular; D004261:DNA Replication; D004271:DNA, Fungal / Q000096:biosynthesis / Q000235:genetics; D004272:DNA, Mitochondrial / Q000096:biosynthesis / Q000235:genetics; D008969:Molecular Sequence Data; D010957:Plasmids; D012091:Repetitive Sequences, Nucleic Acid; D012093:Replicon; D012441:Saccharomyces cerevisiae / Q000235:genetics
D004271:DNA, Fungal; D004272:DNA, Mitochondrial