Factors which affect the activity of purified rat liver acyl-CoA oxidase.
The activity of the enzyme acyl-CoA oxidase (EC 1.3.99.3) is influenced by detergents. At concentrations above the critical micellar concentration, Triton X-100, Triton X-114 and Thesit stimulate oxidase activity. Lower concentrations of Triton X-100 and Triton X-114 render the acyl-CoA oxidase less sensitive towards substrate inhibition by palmitoyl-CoA or dec-4-cis-enoyl-CoA. Other detergents inhibited the enzyme activity. CoA was found to be a relatively powerful competitive inhibitor of the enzyme, with a Ki,slope value of 63 +/- 3 microM. This inhibition is dependent on an intact CoA molecule, as dephospho-CoA, dethio-CoA and acetyl-CoA are less potent inhibitors of the enzyme. Dec-2-trans-enoyl-CoA is a product-inhibitor of acyl-CoA oxidase, with a Ki,slope value of 7 +/- 1 microM.
Hovik
R
R
Department of Physiology and Biochemistry, Dental School, University of Oslo, Norway.
Osmundsen
H
H
D042962:Acyl-CoA Oxidase; D000818:Animals; D001667:Binding, Competitive; D003065:Coenzyme A / Q000494:pharmacology; D003902:Detergents / Q000494:pharmacology; D005227:Fatty Acids / Q000494:pharmacology; D005944:Glucosamine / Q000031:analogs & derivatives / Q000494:pharmacology; D005960:Glucosides / Q000494:pharmacology; D008099:Liver / Q000201:enzymology*; D008297:Male; D009243:NAD / Q000378:metabolism; D017830:Octoxynol; D010088:Oxidoreductases / Q000037:antagonists & inhibitors / Q000302:isolation & purification / Q000378:metabolism*; D010171:Palmitoyl Coenzyme A / Q000494:pharmacology; D000077423:Polidocanol; D011092:Polyethylene Glycols / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar
D003902:Detergents; D005227:Fatty Acids; D005960:Glucosides; D000077423:Polidocanol; D009243:NAD; D010171:Palmitoyl Coenzyme A; D011092:Polyethylene Glycols; C042221:decanoyl-N-methylglucamide; D017830:Octoxynol; C010615:Nonidet P-40; D010088:Oxidoreductases; D042962:Acyl-CoA Oxidase; D005944:Glucosamine; D003065:Coenzyme A