Antibody-catalyzed degradation of cocaine.
Immunization with a phosphonate monoester transition-state analog of cocaine provided monoclonal antibodies capable of catalyzing the hydrolysis of the cocaine benzoyl ester group. An assay for the degradation of radiolabeled cocaine identified active enzymes. Benzoyl esterolysis yields ecgonine methyl ester and benzoic acid, fragments devoid of cocaine's stimulant activity. Passive immunization with such an artificial enzyme could provide a treatment for dependence by blunting reinforcement.
Landry
D W
DW
Department of Medicine, Columbia University College of Physicians and Surgeons, New York, NY 10032.
Zhao
K
K
Yang
G X
GX
Glickman
M
M
Georgiadis
T M
TM
D000818:Animals; D017151:Antibodies, Catalytic / Q000378:metabolism* / Q000627:therapeutic use; D000911:Antibodies, Monoclonal / Q000378:metabolism* / Q000627:therapeutic use; D000941:Antigens / Q000276:immunology; D001565:Benzoates / Q000378:metabolism; D019817:Benzoic Acid; D003042:Cocaine / Q000031:analogs & derivatives / Q000276:immunology / Q000378:metabolism*; D004952:Esters / Q000378:metabolism; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D006868:Hydrolysis; D007114:Immunization; D007116:Immunization, Passive; D051379:Mice; D008807:Mice, Inbred BALB C; D019966:Substance-Related Disorders / Q000188:drug therapy
D017151:Antibodies, Catalytic; D000911:Antibodies, Monoclonal; D000941:Antigens; D001565:Benzoates; D004952:Esters; D019817:Benzoic Acid; D003042:Cocaine; C034524:ecgonine methyl ester