title: a/alpha-control of DNA repair in the yeast Saccharomyces cerevisiae: genetic and physiological aspects.
abstract: It has long been known that diploid strains of yeast are more resistant to gamma-rays than haploid cells, and that this is in part due to heterozygosity at the mating type (MAT) locus. It is shown here that the genetic control exerted by the MAT genes on DNA repair involves the a1 and alpha 2 genes, in a RME1-independent way. In rad18 diploids, affected in the error-prone repair, the a/alpha effects are of a very large amplitude, after both UV and gamma-rays, and also depends on a1 and alpha 2. The coexpression of a and alpha in rad18 haploids suppresses the sensitivity of a subpopulation corresponding to the G2 phase cells. Related to this, the coexpression of a and alpha in RAD+ haploids depresses UV-induced mutagenesis in G2 cells. For srs2 null diploids, also affected in the error-prone repair pathway, we show that their G1 UV sensitivity, likely due to lethal recombination events, is partly suppressed by MAT homozygosity. Taken together, these results led to the proposal that a1-alpha 2 promotes a channeling of some DNA structures from the mutagenic into the recombinational repair process.
authors.affiliation: Institut Curie-Biologie, Centre Universitaire, Orsay, France.
authors.forename: M
authors.initials: M
authors.lastname: Heude
authors.forename: F
authors.initials: F
authors.lastname: Fabre
mesh_terms: D004260:DNA Repair / Q000235:genetics* / Q000502:physiology; D004171:Diploidy; D017353:Gene Deletion; D005800:Genes, Fungal; D049770:Genes, Mating Type, Fungal; D006238:Haploidy; D007399:Interphase; D009154:Mutation; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000502:physiology / Q000528:radiation effects; D014466:Ultraviolet Rays