A functional map of the fruit-specific promoter of the tomato 2A11 gene.
The 5' region of the fruit-specific tomato gene, 2A11, contains both positive and negative regulatory elements. We divided the 5' promoter region of the 2A11 gene into small fragments, ranging in size from 211 to 634 bp and used these short DNA fragments in in vitro protein-binding studies. These studies revealed the presence of at least four fruit-specific and one leaf- and fruit-active protein-binding domains. These promoter fragments, as well as other overlapping fragments, were tested for their ability to enhance expression from a truncated heterologous promoter in transgenic plants. This analysis showed the presence of four fruit-specific and three general or leaf-active positive regulatory elements. Comparison of the results obtained with these two approaches allowed us to draw a functional map of the 2A11 promoter.
Van Haaren
M J
MJ
Calgene Inc., Davis, CA 95616.
Houck
C M
CM
D001665:Binding Sites; D003001:Cloning, Molecular; D015870:Gene Expression; D005786:Gene Expression Regulation; D017343:Genes, Plant; D066298:In Vitro Techniques; D009687:Nuclear Proteins / Q000235:genetics; D030821:Plants, Genetically Modified / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics; D012045:Regulatory Sequences, Nucleic Acid; D015183:Restriction Mapping
D009687:Nuclear Proteins; D012333:RNA, Messenger