Nucleotide sequence of cDNA encoding the fire ant venom protein Sol i II.
For the first time the cDNA encoding a fire ant venom protein has been sequenced. Oligonucleotides were designed according to the amino acid sequence. The cDNA sequence was obtained by hybridizing these primers to mRNA and enhancement by the PCR technique. Comparison to the amino acid sequence of the venom protein shows a leader sequence 19 amino acids long.
Schmidt
M
M
Department of Pathology, East Carolina University, School of Medicine, Greenville, NC 27834.
Walker
R B
RB
Hoffman
D R
DR
McConnell
T J
TJ
D000595:Amino Acid Sequence; D000862:Ant Venoms / Q000737:chemistry* / Q000235:genetics*; D001483:Base Sequence; D004247:DNA / Q000737:chemistry* / Q000235:genetics; D019476:Insect Proteins; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D014169:Transformation, Bacterial
D000862:Ant Venoms; D019476:Insect Proteins; C080289:Sol i II protein, Solenopsis invicta; D004247:DNA