Differential expression of serotonin and [Met]enkephalin-Arg6-Gly7-Leu8 in neurons of the rat brain stem.
The hypothesis that serotonin (5-HT) and [Met]enkephalin-Arg6-Gly7-Leu8 (MEAGL) coexist in the rat brain stem raphe nuclei was tested by a technique combining histofluorescence with immunocytochemistry, after treatment with colchicine and nialamide. In midbrain and pons serotonergic cell groups (B5-B9), no coexistence of 5-HT and MEAGL was detected. In serotonergic cell groups of the medulla oblongata (B1-B3), only 0.3-1.5% of 5-HT-fluorescent cells were MEAGL-immunoreactive. These findings suggest that putative 5-HT and MEAGL are mostly expressed in different populations of neurons in the rat raphe nuclei.
Tanaka
M
M
Department of Anesthesiology, Kyoto Prefectural University of Medicine, Japan.
Okamura
H
H
Yanaihara
N
N
Tanaka
Y
Y
Ibata
Y
Y
D000818:Animals; D001933:Brain Stem / Q000166:cytology / Q000378:metabolism*; D003078:Colchicine / Q000008:administration & dosage / Q000494:pharmacology; D004744:Enkephalin, Methionine / Q000031:analogs & derivatives* / Q000096:biosynthesis; D005455:Fluorescent Antibody Technique; D007150:Immunohistochemistry; D007276:Injections, Intraventricular; D008297:Male; D009474:Neurons / Q000378:metabolism*; D009526:Nialamide / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D012701:Serotonin / Q000096:biosynthesis* / Q000378:metabolism
D012701:Serotonin; D004744:Enkephalin, Methionine; C033453:enkephalin-Met, Arg(6)-Gly(7)-Leu(8)-; D003078:Colchicine; D009526:Nialamide