title: Cyclic ADP-ribose-induced Ca2+ release from rat brain microsomes.
abstract: Cyclic ADP-ribose (cADPR), an endogenous NAD+ metabolite in many mammalian and invertebrate tissues, is a potent mediator of calcium mobilization in sea urchin eggs. Our results show that cADPR also stimulates calcium release from rat brain microsomes, marked release occurring over the concentration range 10-250 nM. This is not inhibited by concentrations of heparin which completely abolish inositol 1,4,5-trisphosphate (IP3)-induced Ca2+ release. Ryanodine (100 microM) inhibits the cADPR response. Our results are consistent with cADPR being an endogenous messenger mediating Ca2+ release from ryanodine-sensitive pools in brain.
authors.affiliation: Department of Pharmacology, University of Oxford, UK.
authors.forename: A M
authors.initials: AM
authors.lastname: White
authors.forename: S P
authors.initials: SP
authors.lastname: Watson
authors.forename: A
authors.initials: A
authors.lastname: Galione
mesh_terms: D000246:Adenosine Diphosphate Ribose / Q000031:analogs & derivatives* / Q000494:pharmacology; D000818:Animals; D001921:Brain / Q000187:drug effects / Q000648:ultrastructure*; D002118:Calcium / Q000378:metabolism*; D036563:Cyclic ADP-Ribose; D006493:Heparin / Q000494:pharmacology; D006358:Hot Temperature; D015544:Inositol 1,4,5-Trisphosphate / Q000378:metabolism; D008297:Male; D008861:Microsomes / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D012433:Ryanodine / Q000494:pharmacology
chemical_list: D036563:Cyclic ADP-Ribose; D012433:Ryanodine; D000246:Adenosine Diphosphate Ribose; D015544:Inositol 1,4,5-Trisphosphate; D006493:Heparin; D002118:Calcium