title: A new 'solid phase' procedure to synthesize immunotoxins (antibody-ribosome inactivating protein conjugates).
abstract: A method to produce immunotoxins (conjugates comprising of a monoclonal antibody and toxin) using ribosome inactivating protein anchored on an affinity gel derivatized with triazinic dye is described. The adsorbed toxins were activated with 2-imino-thiolane and then conjugated to monoclonal antibody activated by SPDP. The "heterogeneous phase" system offered several advantages, reducing the usually required purification steps and opening a way to automatize the conjugation procedure.
authors.affiliation: Istituto di Chimica Farmaceutica Applicata, Torino, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Dosio
authors.forename: P
authors.initials: P
authors.lastname: Brusa
authors.forename: L
authors.initials: L
authors.lastname: Delprino
authors.forename: M
authors.initials: M
authors.lastname: Ceruti
authors.forename: G
authors.initials: G
authors.lastname: Grosa
authors.forename: L
authors.initials: L
authors.lastname: Cattel
authors.forename: A
authors.initials: A
authors.lastname: Bolognesi
authors.forename: L
authors.initials: L
authors.lastname: Barbieri
mesh_terms: D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000276:immunology; D002474:Cell-Free System; D002850:Chromatography, Gel; D006801:Humans; D000922:Immunotoxins / Q000737:chemistry* / Q000633:toxicity; D010940:Plant Proteins / Q000737:chemistry / Q000276:immunology; D014176:Protein Biosynthesis; D011500:Protein Synthesis Inhibitors / Q000737:chemistry / Q000276:immunology; D054789:Ribosome Inactivating Proteins, Type 1; D014407:Tumor Cells, Cultured / Q000187:drug effects
chemical_list: D000911:Antibodies, Monoclonal; D000922:Immunotoxins; D010940:Plant Proteins; D011500:Protein Synthesis Inhibitors; D054789:Ribosome Inactivating Proteins, Type 1; C025674:GEL protein, Gelonium multiflorum