Functional epidermal growth factor receptor localizes to the postacrosomal region of human spermatozoa.
The human epidermal growth factor receptor was detected immunohistochemically in the postacrosomal region of ejaculated human sperm and, when immunopurified and exposed to [gamma-32P]ATP, was found to be autophosphorylated. Localization of an apparently enzymatically active epidermal growth factor receptor to spermatozoa suggests that epidermal growth factor receptor-ligand interactions may play a role in gamete physiology or in fertilization.
Damjanov
I
I
Department of Pathology, Thomas Jefferson University, Philadelphia, PA 19107.
Solter
D
D
Knowles
B B
BB
D000911:Antibodies, Monoclonal; D002451:Cell Compartmentation; D066246:ErbB Receptors / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D006801:Humans; D008297:Male; D010766:Phosphorylation; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D013094:Spermatozoa / Q000378:metabolism* / Q000648:ultrastructure
D000911:Antibodies, Monoclonal; D066246:ErbB Receptors; D011505:Protein-Tyrosine Kinases