Monoclonal antibodies against methionyl recombinant human prolactin.
Hybridoma cell lines producing monoclonal antibody (Mab) against recombinant human prolactin (rhPrl) were established from fusion between X63-Ag8 myeloma cells and Balb/c mice splenocytes. Four Mabs numbered I to IV were selected by ELISA, purified and characterized. All these Mabs were of the Ig1 kappa isotype and able to recognize oxidized as well as reduced rhPrl. As shown by a competitive inhibition assay, Mab IV did not compete with any of the three others. Moreover, both rhPrl and hPrl extracted from human pituitaries, were recognized equally by this Mab. Properties displayed by Mab IV make it very attractive for the evaluation of prolactin levels by sandwich immunoassays.
Paris
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Laboratoire de Biochimie Générale, UFR Sciences de l'Environnement, Université d'Angers, France.
Robert
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Mercier
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D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001667:Binding, Competitive; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007118:Immunoassay; D007074:Immunoglobulin G / Q000276:immunology; D007145:Immunoglobulin kappa-Chains / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology; D011388:Prolactin / Q000031:analogs & derivatives* / Q000276:immunology; D011993:Recombinant Fusion Proteins / Q000276:immunology*; D011994:Recombinant Proteins
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D007145:Immunoglobulin kappa-Chains; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; C060530:methionylprolactin; D011388:Prolactin