title: Available lambda B cell repertoire in the mouse: evidence of positive selection by environmental factors.
abstract: We have recently shown that, from two BALB/c mice treated with rabbit anti-C lambda 2/C lambda 3 antibodies coupled to lipopolysaccharide, variable heavy chain (VH) family repertoires associated with lambda 2 or lambda 3 light chains can differ from one lambda subtype to another and from one individual mouse to another. Indeed, 4 out of 6 lambda 2 (VxJ2) hybridomas from one mouse preferentially expressed the VH10 family while 3 out of 8 lambda 2 (V2J2) and 5 out of 8 lambda 2 (VxJ2) hybridomas from a second mouse preferentially expressed the S107 and VGAM3.8 VH families, respectively. In this report, we describe the structural basis of such preferential pairings by sequence analysis of the 12 lambda 2 hybridomas. The sequence comparison of their VH regions show that each preferential association of a VH family to one V lambda region is restricted to the use of a single member or very closely related members inside a VH family and that a great variability of CDR3 of heavy chain is observed. We, therefore, suggest that environmental factors can modify the available lambda B cell repertoire through a positive selection of particular VH/V lambda pairings. Moreover, our data support that this selection does not require clonal expansion and punctual somatic mutation.
authors.affiliation: Département d'Immunologie, Institut Pasteur, Paris, France.
authors.forename: B
authors.initials: B
authors.lastname: Nadel
authors.forename: A M
authors.initials: AM
authors.lastname: Drapier
authors.forename: P A
authors.initials: PA
authors.lastname: Cazenave
authors.forename: P
authors.initials: P
authors.lastname: Sanchez
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000916:Antibody Diversity / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology*; D001483:Base Sequence; D005803:Genes, Immunoglobulin / Q000276:immunology; D006825:Hybridomas / Q000276:immunology; D007146:Immunoglobulin lambda-Chains / Q000276:immunology*; D008070:Lipopolysaccharides; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction; D012641:Selection, Genetic
chemical_list: D007146:Immunoglobulin lambda-Chains; D008070:Lipopolysaccharides; D015345:Oligonucleotide Probes