title: Inhibition of the vitamin B12 binding capacity of proteins by the hydrolysis product of cyclophosphamide.
abstract: The inhibitory effect of cyclophosphamide hydrolysis product (CPHP) on vitamin B12 binding ability to proteins has been established. The ester N-(2-chloroethyl)-N'-(3-phosphopropyl)-ethylenediamine hydrochloride is probably responsible, in vitro, for blocking the protein binding sites. Preincubation of proteins with vitamin B12 prevents the inhibitory effect of CPHP.
authors.affiliation: Department of Biochemistry, K. Marcinkowski University School of Medicine, Poznań, Poland.
authors.forename: W
authors.initials: W
authors.lastname: Fenrych
authors.forename: E
authors.initials: E
authors.lastname: Szczodrowska
authors.forename: E
authors.initials: E
authors.lastname: Ignatowicz
mesh_terms: D001798:Blood Proteins / Q000378:metabolism*; D003520:Cyclophosphamide / Q000737:chemistry / Q000494:pharmacology*; D005029:Ethylenediamines / Q000494:pharmacology*; D006801:Humans; D006868:Hydrolysis; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D010755:Organophosphates / Q000494:pharmacology*; D011485:Protein Binding / Q000187:drug effects; D014805:Vitamin B 12 / Q000378:metabolism*
chemical_list: D001798:Blood Proteins; D005029:Ethylenediamines; D010755:Organophosphates; C079884:N-(2-chloroethyl)-N'-(3-phosphopropyl)ethylenediamine; D003520:Cyclophosphamide; D014805:Vitamin B 12