Nucleotide sequences of the Macaca fascicularis apolipoprotein C-III and A-IV genes.
The cynomolgus monkey (Macaca fascicularis) apolipoprotein C-III and apolipoprotein A-IV genes have been isolated from a cynomolgus genomic DNA library and completely sequenced. These genes span 3.1 and 2.8 kilobases (kb), respectively. Apolipoprotein C-III gene is interrupted by three intervening sequences of 613, 135 and 1699 bp, respectively. The open reading frame encodes a protein of 99 amino acids which is 87% similar to the human. The cynomolgus mature protein is 79 residues long. Thr-74 is also present what might allow the formation of O-glycosidic linkage observed in the human protein. Apolipoprotein A-IV gene consist of two intervening sequences of 352 and 774 bp, respectively. The open reading frame encodes a protein of 429 amino acids which is 87% similar to the human. The cynomolgus mature protein is 409 residues long, 33 amino acids longer than the human, due to an insertion of 33 residues in its COOH-terminal region. This insertion is mainly composed of glutamine and glutamic acid, which confers cynomolgus apolipoprotein a higher hydrophilicity.
Osada
J
J
Department of Clinical Sciences, University of Lowell, MA.
Pocoví
M
M
Nicolosi
R J
RJ
Schaefer
E J
EJ
Ordovás
J M
JM
D000595:Amino Acid Sequence; D000818:Animals; D053305:Apolipoprotein C-III; D001054:Apolipoproteins A / Q000235:genetics*; D001056:Apolipoproteins C / Q000235:genetics*; D001483:Base Sequence; D006801:Humans; D007438:Introns; D008252:Macaca fascicularis / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D017386:Sequence Homology, Amino Acid
D053305:Apolipoprotein C-III; D001054:Apolipoproteins A; D001056:Apolipoproteins C; C022205:apolipoprotein A-IV