Fast DNA isolation from Histoplasma capsulatum: methodology for arbitrary primer polymerase chain reaction-based epidemiological and clinical studies.
The arbitrary primer polymerase chain reaction (also called random amplified polymorphic DNA, or RAPD) is a DNA fingerprinting method that provides an efficient, sensitive way of discriminating between independent isolates of Histoplasma capsulatum, but its widespread application has been hampered by the arduous 2-day procedure traditionally used to extract DNA from H. capsulatum. We present here a quick (approximately 2-h) extraction method and show that the resultant DNA is suitable for sensitive and reproducible identification of individual strains of this pathogenic fungus.
Woods
J P
JP
Department of Molecular Microbiology, Washington University Medical School, St. Louis, Missouri 63110.
Kersulyte
D
D
Goldman
W E
WE
Berg
D E
DE
D001483:Base Sequence; D016172:DNA Fingerprinting / Q000379:methods / Q000706:statistics & numerical data; D004271:DNA, Fungal / Q000235:genetics* / Q000302:isolation & purification*; D005069:Evaluation Studies as Topic; D006658:Histoplasma / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008969:Molecular Sequence Data; D009172:Mycology / Q000379:methods / Q000706:statistics & numerical data; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000706:statistics & numerical data; D012680:Sensitivity and Specificity
D004271:DNA, Fungal