The hyaluronate synthase from a eukaryotic cell line.
The hyaluronate synthase complex was identified in plasma membranes from B6 cells. It contained two subunits of molecular masses 52 kDa and 60 kDa which bound the precursor UDP-GlcA in digitonin solution and partitioned into the aqueous phase, together with nascent hyaluronate upon Triton X-114 phase separation. The 52 kDa protein cross-reacted with poly- and monoclonal antibodies raised against the streptococcal hyaluronate synthase and the 60 kDa protein was recognized by monoclonal antibodies raised against a hyaluronate receptor. The 52 kDa protein was purified to homogeneity by affinity chromatography with monoclonal anti-hyaluronate synthase.
Klewes
L
L
Institute für Physiologische Chemie und Pathobiochemie, Münster, Germany.
Turley
E A
EA
Prehm
P
P
D000818:Animals; D000911:Antibodies, Monoclonal; D015153:Blotting, Western; D002462:Cell Membrane / Q000201:enzymology*; D002846:Chromatography, Affinity; D006224:Cricetinae; D004797:Enzyme-Linked Immunosorbent Assay; D005944:Glucosamine / Q000031:analogs & derivatives / Q000378:metabolism; D014453:Glucuronosyltransferase / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D016695:Glycosyltransferases; D000076002:Hyaluronan Synthases; D006822:Hybrid Cells; D008168:Lung; D008325:Mammary Neoplasms, Experimental; D008565:Membrane Proteins; D051379:Mice; D008970:Molecular Weight; D014166:Transferases; D014407:Tumor Cells, Cultured; D014537:Uridine Diphosphate N-Acetylglucosamine / Q000378:metabolism; D029867:Xenopus Proteins
D000911:Antibodies, Monoclonal; D008565:Membrane Proteins; D029867:Xenopus Proteins; C026712:UDP-glucosamine; D014537:Uridine Diphosphate N-Acetylglucosamine; D014166:Transferases; D016695:Glycosyltransferases; D014453:Glucuronosyltransferase; C056944:HAS1 protein, Xenopus; D000076002:Hyaluronan Synthases; D005944:Glucosamine