title: Analysis of sequence variation in grapevine yellow speckle viroid 1 reveals two distinct alternative structures for the pathogenic domain.
abstract: The nucleotide sequences of 24 full-length cDNA clones prepared from a field isolate of grapevine yellow speckle viroid 1 (GYSV 1) have been determined and compared with that of the previously published GYSV 1 sequence. A large number of sequence variations were observed, the majority of which occurred in the pathogenicity domain (P domain) of the viroid. The GYSV 1 variants could be divided into two types each containing a distinct secondary structure within the P domain. Monomeric exact-length RNA transcripts produced from individual clones belonging to both types were infectious in viroid-free grapevines and produced homogenous populations of progeny viroid in vivo.
authors.affiliation: CSIRO Division of Horticulture, Adelaide, Australia.
authors.forename: J E
authors.initials: JE
authors.lastname: Rigden
authors.forename: M A
authors.initials: MA
authors.lastname: Rezaian
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular; D005638:Fruit / Q000382:microbiology; D014644:Genetic Variation / Q000235:genetics*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010942:Plant Viruses / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics; D014772:Viroids / Q000235:genetics*
chemical_list: D012367:RNA, Viral