Use of particulate forms of protein antigens to increase the sensitivity of antigen-specific proliferation assays.
Different forms of recombinant HIV-1 gp160 and tetanus toxoid were adsorbed onto latex microspheres and this particulate form of the proteins was used to measure antigen-specific proliferation in vaccinated rhesus macaques. Proliferative responses to proteins bound to microspheres were significantly greater and allowed for the detection of antigen-specific responses that were not detected using soluble proteins. The responses were antigen-specific and required prior immunization of the animals. Additionally, the presence and magnitude of the proliferative responses was associated with antibody responses to the same proteins suggesting the results were representative of in vivo responses and that the assay format did not induce in vitro artifacts.
Wu
J Y
JY
Cambridge Biotech Corporation, Worcester, MA 01605.
Munroe
K J
KJ
Gardner
B H
BH
Wyand
M S
MS
Newman
M J
MJ
D016915:AIDS Vaccines / Q000276:immunology; D000818:Animals; D000941:Antigens / Q000008:administration & dosage / Q000276:immunology*; D015686:Gene Products, env / Q000276:immunology; D015483:HIV Antibodies / Q000096:biosynthesis; D019302:HIV Envelope Protein gp160; D008213:Lymphocyte Activation; D008253:Macaca mulatta; D008297:Male; D008863:Microspheres; D011498:Protein Precursors / Q000276:immunology; D012680:Sensitivity and Specificity; D013745:Tetanus Toxoid / Q000276:immunology
D016915:AIDS Vaccines; D000941:Antigens; D015686:Gene Products, env; D015483:HIV Antibodies; D019302:HIV Envelope Protein gp160; D011498:Protein Precursors; D013745:Tetanus Toxoid