Localization of the human poly(A)-binding protein gene (PAB1) to chromosomal regions 3q22-q25, 12q13-q14, and 13q12-q13 by in situ hybridization.
A cDNA clone encoding the human polyadenylate-binding protein (PABP) was isolated and mapped to the genome by in situ hybridization. The cDNA was found to bind preferentially to chromosomal regions 3q22-q25, 12q13-q14, and 13q12-q13. In addition, Southern blot analysis of genomic DNA using a PABP cDNA probe revealed a complex pattern of bands. The results indicate that PABP belongs to a multigene family of related sequences.
Morris
C M
CM
Department of Pathology, University of Otago, Christchurch Hospital, New Zealand.
Bodger
M P
MP
D015139:Blotting, Southern; D002352:Carrier Proteins / Q000235:genetics*; D002874:Chromosome Mapping; D002881:Chromosomes, Human, Pair 12; D002882:Chromosomes, Human, Pair 13; D002893:Chromosomes, Human, Pair 3; D004247:DNA; D005260:Female; D006801:Humans; D017403:In Situ Hybridization; D008297:Male; D039102:Poly(A)-Binding Proteins
D002352:Carrier Proteins; D039102:Poly(A)-Binding Proteins; D004247:DNA