Alpha (p55) and beta (p75) chains of the interleukin-2 receptor are expressed by AML blasts.
In the present study, we have investigated the leukemic cells obtained from 16 patients with acute myeloid leukemia (AML) at diagnosis for the membrane expression of p55 (alpha) and p75 (beta) interleukin-2 receptor (IL-2R) chains using specific monoclonal antibodies (mAbs), as well as for the presence of their transcripts using Northern blot analysis. In addition, immunoprecipitation of the p75 membrane molecule with TU27 and Mik-beta 1 mAbs was carried out in selected cases. The p75 IL-2R beta transcripts were detected in all cases, whereas the membrane p75 molecule was demonstrable by flow cytometry in three cases. However, data from the immunoprecipitation analysis suggest that the lack of the p75 IL-2R detection by flow cytometry might be caused by the low density of molecules per cell rather than the fact that the specific mRNA is not translated into the p75 surface molecule. In addition, a consistent membrane positivity with an anti-p55/CD25 mAb, present on fresh uncultured blasts in 37.5% of the cases, became detectable after short-term culture in 75% of cases. In each individual case, a strict correlation was found between membrane CD25 reactivity and the expression of p55 mRNA. Taken together, our data suggest that the expression of both alpha (p55) and beta (p75) IL-2R molecules is a common feature of leukemic cells in AML, and provide new arguments for reassessing the possible role of IL-2 in leukemic growth.
Pizzolo
G
G
Department of Hematology, Verona University School of Medicine, Italy.
Rigo
A
A
Zanotti
R
R
Vinante
F
F
Vincenzi
C
C
Cassatella
M
M
Carra
G
G
Castaman
G
G
Chilosi
M
M
Semenzato
G
G
D000208:Acute Disease; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D015152:Blotting, Northern; D006801:Humans; D007948:Leukemia, Monocytic, Acute / Q000235:genetics / Q000473:pathology / Q000503:physiopathology; D007951:Leukemia, Myeloid / Q000235:genetics / Q000473:pathology* / Q000503:physiopathology; D015470:Leukemia, Myeloid, Acute / Q000235:genetics / Q000473:pathology / Q000503:physiopathology; D015479:Leukemia, Myelomonocytic, Acute / Q000235:genetics / Q000473:pathology / Q000503:physiopathology; D015473:Leukemia, Promyelocytic, Acute / Q000235:genetics / Q000473:pathology / Q000503:physiopathology; D046911:Macromolecular Substances; D008565:Membrane Proteins / Q000235:genetics; D009363:Neoplasm Proteins / Q000235:genetics; D011233:Precipitin Tests; D012333:RNA, Messenger / Q000235:genetics; D015375:Receptors, Interleukin-2 / Q000032:analysis* / Q000235:genetics / Q000502:physiology; D014158:Transcription, Genetic / Q000235:genetics
D000911:Antibodies, Monoclonal; D046911:Macromolecular Substances; D008565:Membrane Proteins; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D015375:Receptors, Interleukin-2