Determination of acidic saponins in crude drugs by high-performance liquid chromatography on octadecylsilyl porous glass.
High-performance liquid chromatographic (HPLC) analysis on octadecylsilyl porous glass was investigated for acidic saponins in ginseng, bupleurum root and senega. The acidic saponins, malonyl-ginsenosides, malonyl-saikosaponins and senegins, as well as neutral saponins in the crude drugs were separated rapidly by HPLC on this column with aqueous acetonitrile containing KH2PO4 as the mobile phase at room temperature.
Kanazawa
H
H
Kyoritsu College of Pharmacy, Tokyo, Japan.
Nagata
Y
Y
Matsushima
Y
Y
Tomoda
M
M
Takai
N
N
D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005898:Glass; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D015394:Molecular Structure; D005894:Panax / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D012503:Saponins / Q000032:analysis* / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet
D012503:Saponins