title: [Study of the gene of Duchenne muscular dystrophy using PCR analysis].
abstract: We have performed the polymerase chain reaction (PCR) to study 26 patients affected with Duchenne muscular dystrophy as a direct diagnostic method of screening for gene deletions. We have amplified simultaneously 9 sequences which belong to 9 exons located along the two "hot spots" of the gene. We have detected deletions in many of the exons in 11 of the 26 patients (42.3% of the cases).
authors.affiliation: Departamento de Genética, Fundación Jiménez Díaz, Madrid.
authors.forename: M
authors.initials: M
authors.lastname: Villamar López
authors.forename: E
authors.initials: E
authors.lastname: Fernández García
authors.forename: C
authors.initials: C
authors.lastname: Ramos Corrales
authors.forename: J
authors.initials: J
authors.lastname: Benítez Ortiz
mesh_terms: D015139:Blotting, Southern; D002648:Child; D002872:Chromosome Deletion; D004274:DNA, Recombinant; D005260:Female; D017353:Gene Deletion; D006801:Humans; D017403:In Situ Hybridization; D008297:Male; D009136:Muscular Dystrophies / Q000235:genetics*; D016133:Polymerase Chain Reaction
chemical_list: D004274:DNA, Recombinant