Antibodies to recombinant fragment 212-276 of protein C specifically recognize the intact human molecule.
The peptide fragment Pro212-Ile276 of human protein C was produced as a part of a fusion protein in Escherichia coli. The identity of the peptide was confirmed by immunoblotting experiments using specific antibodies to intact protein C. The peptide Pro212-Ile276 was isolated from the fusion protein after mild hydrolysis with formic acid by gel filtration and reverse-phase HPLC. This peptide fragment was used to produce antibodies specific for the heavy chain of protein C which recognized native protein C present in blood plasma. Antibodies to intact protein C reacted also with the Pro212-Ile276 peptide fragment, indicating that this region is immunogenic in intact protein C and may represent a native epitope.
Fijalkowska
I
I
Microbiology and Virusology Center, Lodz, Poland.
Babinska
A
A
Ligocka
A
A
Plucienniczak
G
G
Plucienniczak
A
A
Stec
W
W
Okruszek
A
A
Wilk
A
A
Cierniewski
C S
CS
D000595:Amino Acid Sequence; D000918:Antibody Specificity; D001483:Base Sequence; D001667:Binding, Competitive; D015153:Blotting, Western; D002851:Chromatography, High Pressure Liquid; D004274:DNA, Recombinant; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli; D006801:Humans; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000276:immunology* / Q000378:metabolism; D011486:Protein C / Q000276:immunology* / Q000378:metabolism; D011994:Recombinant Proteins / Q000276:immunology / Q000378:metabolism
D004274:DNA, Recombinant; D010446:Peptide Fragments; D011486:Protein C; D011994:Recombinant Proteins