O,O,S-Trimethyl phosphorothioate induces hypothermia in Fischer 344 rats in a manner dependent on both doses and housing temperatures.
We explored the effects of O,O,S-trimethyl phosphorothioate (OOS-TMP) on body temperatures in Fischer 344 female rats. The 7-day LD50 p.o. for Fischer 344 female rats was found to be 11.8 mg/kg. OOS-TMP induced long-lasting (more than 48 h) and extensive hypothermia at doses > or = 14 mg/kg at a typical laboratory temperature (22 degrees C) while it produced typical symptoms at 10 mg/kg without hypothermia. In contrast, pair-fed (to 20 mg/kg rats) rats (n = 4) did not become hypothermic, negating any role of hypophagia in OOS-TMP associated hypothermia. We next investigated the effects of housing temperatures on toxicities at a LD50 dose (12 mg/kg). At 30 degrees C (n = 11) and 22 degrees C (n = 13), rats did not have hypothermic bouts but at 15 degrees C, eight out of ten rats had. Evidence that changes of housing temperatures neither modified clinical symptoms nor changed mortality rates discards a possibility of hypothermia being involved in delayed toxicity. A novel result of the present study suggests that thermoregulation may be heavily impaired by a special class of organophosphorus compounds.
Hamade
N
N
Department of Hygiene, Akita University School of Medicine, Japan.
Jin
Y
Y
Tsukada
M
M
Wada
Y
Y
Koizumi
A
A
D000818:Animals; D001831:Body Temperature / Q000187:drug effects; D005260:Female; D007035:Hypothermia / Q000139:chemically induced* / Q000503:physiopathology; D007928:Lethal Dose 50; D063086:Organothiophosphates / Q000633:toxicity*; D051381:Rats; D011916:Rats, Inbred F344; D013696:Temperature
D063086:Organothiophosphates; C019123:O,O,S-trimethyl phosphorothioate