L-gulono-gamma-lactone oxidase is the enzyme responsible for the production of methylguanidine in the rat liver.
A methylguanidine-synthesizing enzyme localized in rat liver microsomes produces methylguanidine via the intermediates creatone A and creatone B from the substrate, creatol, a substance produced from creatinine mainly by reaction with hydroxyl radicals. This enzyme has been identified as L-gulono-gamma-lactone oxidase (EC 1.1.3.8). However, no corresponding activity was found in extracts from human livers.
Fujitsuka
N
N
Research Institute for Wakan-Yaku, Toyama Medical and Pharmaceutical University, Sugitani, Japan.
Yokozawa
T
T
Oura
H
H
Akao
T
T
Kobashi
K
K
Ienaga
K
K
Nakamura
K
K
D000595:Amino Acid Sequence; D000818:Animals; D006801:Humans; D066298:In Vitro Techniques; D050544:L-Gulonolactone Oxidase; D008099:Liver / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D008760:Methylguanidine / Q000378:metabolism*; D008862:Microsomes, Liver / Q000201:enzymology; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010101:Oxygen Consumption; D051381:Rats; D017208:Rats, Wistar; D013045:Species Specificity; D013347:Subcellular Fractions / Q000201:enzymology; D013401:Sugar Alcohol Dehydrogenases / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*
D008760:Methylguanidine; D013401:Sugar Alcohol Dehydrogenases; D050544:L-Gulonolactone Oxidase