Deregulated c-fos augments cell proliferation of B cells mediated by lipopolysaccharide.
We have examined effects of the deregulated c-fos protein on the lipopolysaccharide (LPS)-mediated B cell responses using splenic B cells from transgenic lines carrying the mouse c-fos gene under the control of the H-2K (H2-c-fos) and the inducible Mx promoter (Mx-c-fosD). High c-fos expression was induced in Mx-c-fosD B cells by LPS stimulation. DNA synthesis of the B cells from both lines was augmented depending on the amount of exogenous c-fos. This augmentation resulted in the increase of IgM and IgG2b productions in the culture. These results suggest a functional role of c-fos protein in cell cycle progression of the activated B cells.
Takada
M
M
Department of Immunology, ICMR, Kobe University School of Medicine, Hyogo, Japan.
Koizumi
T
T
Bachiller
D
D
Rüther
U
U
Tokuhisa
T
T
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D004247:DNA / Q000096:biosynthesis; D005786:Gene Expression Regulation; D007074:Immunoglobulin G / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000096:biosynthesis; D008070:Lipopolysaccharides / Q000494:pharmacology; D008213:Lymphocyte Activation; D051379:Mice; D008822:Mice, Transgenic; D016760:Proto-Oncogene Proteins c-fos; D013154:Spleen / Q000166:cytology
D007074:Immunoglobulin G; D007075:Immunoglobulin M; D008070:Lipopolysaccharides; D016760:Proto-Oncogene Proteins c-fos; D004247:DNA