title: [The isolation and purification of a nonspecific DNAse from Mycoplasma fermentans PG-18].
abstract: Nonspecific endogenic DNAase has been isolated from biomass of Mycoplasma fermentans PG-18 cells and purified to the homogeneous state. The scheme of isolation consists of purification stages on columns with phosphocellulose, DNA-cepharose CL-8B and phenylcepharose. DNAase was not bound to phosphocellulose, its volume was equal to zero. Then this DNAase was passed through column with DEA-cepharose CL-6B (elution by gradient KCl from 0.1 to 1.8M): enzyme was eluted at KCl concentration in the eluting buffer from 0.1 to 1.2 M. The enzyme was purified to the homogeneous state on column with phenylcepharose (elution by linear gradient of ethylene glycol from 30 to 80%): enzyme was eluted at the concentration of ethylene glycol in the eluting buffer from 43 to 80%. According to data obtained using gel-electrophoresis, under the denaturing conditions molecular mass of enzyme in acrylamide gel was 34 kDa.
authors.forename: S Z
authors.initials: SZ
authors.lastname: Sitaĭlo
authors.forename: V V
authors.initials: VV
authors.lastname: Babichev
authors.forename: I G
authors.initials: IG
authors.lastname: Skripal'
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000382:microbiology; D003851:Deoxyribonucleases / Q000032:analysis / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005835:Genitalia / Q000382:microbiology; D006801:Humans; D008970:Molecular Weight; D016858:Mycoplasma fermentans / Q000201:enzymology* / Q000302:isolation & purification
chemical_list: D003851:Deoxyribonucleases