Characterization and chromosomal assignment of a human cDNA encoding a protein related to the murine 102-kDa cadherin-associated protein (alpha-catenin).
We report the characterization of a human cDNA encompassing the complete coding region of a 945-residue putative protein (CAP-R) 80% identical to the recently described murine 102-kDa alpha-catenin (CAP102). The CAP-R protein mostly differs from CAP102 by the presence of a 48-residue insert. This insert exhibits similarity with a segment of the type 1 neurofibromatosis gene product. The analysis of a publicly available human "expressed sequence tag" collection revealed the existence of another human cDNA more closely related (89% identical) to CAP102. This strongly suggests that CAP-R is not the human homologue of the murine 102-kDa alpha-catenin but a new closely related gene of the vinculin family. This is further supported by the computed mutation rates falling outside the range observed for mammalian orthologous genes. Using in situ hybridization, the CAP-R gene could be mapped to the p11.1-p12 region of human chromosome 2 and to the homologous B3-D region of mouse chromosome 6.
Claverie
J M
JM
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, Maryland 20894.
Hardelin
J P
JP
Legouis
R
R
Levilliers
J
J
Bougueleret
L
L
Mattei
M G
MG
Petit
C
C
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D002889:Chromosomes, Human, Pair 2; D003598:Cytoskeletal Proteins / Q000235:genetics*; D004247:DNA; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009154:Mutation; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D016324:Sequence Tagged Sites; D051178:alpha Catenin
C495273:CTNNA1 protein, human; C079671:CTNNA2 protein, human; C495272:Ctnna1 protein, mouse; D003598:Cytoskeletal Proteins; D051178:alpha Catenin; D004247:DNA