An evaluation of the inactive mouse X chromosome in somatic cell hybrids.
The expression of mouse Zfx, Rps4, Ube1x, and Xist was evaluated in hamster-mouse somatic cell hybrids containing either an active or an inactive mouse X chromosome using polymerase chain reaction of reverse transcribed RNA (RT-PCR). The results showed that Zfx, Rps4, and Ube1x are expressed exclusively from the active mouse X, while Xist is expressed exclusively from the inactive X. These findings confirm the pattern of X inactivation for these mouse genes reported previously based on expression in somatic tissues of F1 females from interspecific crosses. These results demonstrate the existence of differences between human and mouse X inactivation, as the corresponding human genes, ZFX, RPS4X, and UBE1 escape X inactivation.
Salido
E C
EC
Department of Pediatrics, University of California, San Francisco 94143.
Passage
M B
MB
Yen
P H
PH
Shapiro
L J
LJ
Mohandas
T K
TK
D000818:Animals; D001483:Base Sequence; D006224:Cricetinae; D003433:Crosses, Genetic; D004277:DNA, Single-Stranded; D004303:Dosage Compensation, Genetic; D005260:Female; D006801:Humans; D006822:Hybrid Cells; D008025:Ligases / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012269:Ribosomal Proteins / Q000737:chemistry / Q000235:genetics; D044767:Ubiquitin-Protein Ligases; D014960:X Chromosome; D016335:Zinc Fingers / Q000235:genetics
D004277:DNA, Single-Stranded; D012269:Ribosomal Proteins; C031798:ribosomal protein S4; D044767:Ubiquitin-Protein Ligases; D008025:Ligases