title: Cloning of genes for proline and leucine biosynthesis from Brucella abortus by functional complementation in Escherichia coli.
abstract: By selecting for growth of Escherichia coli mutant strains in the absence of the required amino acid, clones were found in a Brucella abortus library carrying genes for glutamyl phosphate reductase (proA) and beta-isopropylmalate dehydrogenase (leuB). These clones hybridized to unique fragments in a genomic digest of B. abortus DNA. The proA-complementing DNA was found in a region of 1.3 kb, which directed the synthesis of a protein of 48,000 Da with a pI of 6.3 in maxicells. The leuB-complementing activity was in a region of 1.4 kb and directed synthesis of a protein of 46,000 Da with a pI of 5.9.
authors.affiliation: Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater 74078.
authors.forename: R C
authors.initials: RC
authors.lastname: Essenberg
authors.forename: Y K
authors.initials: YK
authors.lastname: Sharma
mesh_terms: D050539:3-Isopropylmalate Dehydrogenase; D000429:Alcohol Oxidoreductases / Q000032:analysis / Q000235:genetics*; D000445:Aldehyde Oxidoreductases / Q000032:analysis / Q000235:genetics*; D002003:Brucella abortus / Q000235:genetics*; D004252:DNA Mutational Analysis; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial / Q000235:genetics*; D005816:Genetic Complementation Test; D050698:Glutamate-5-Semialdehyde Dehydrogenase; D007930:Leucine / Q000096:biosynthesis*; D011392:Proline / Q000096:biosynthesis*; D015183:Restriction Mapping
chemical_list: D011392:Proline; D000429:Alcohol Oxidoreductases; D050539:3-Isopropylmalate Dehydrogenase; D000445:Aldehyde Oxidoreductases; D050698:Glutamate-5-Semialdehyde Dehydrogenase; D007930:Leucine