Influence of carbicron (O-[(2-butenoic acid)-N,N-dimethylamide-3-yl] O,O-dimethylphosphate) on some biochemical and biophysical parameters of rat liver membranes.
1. Treatment of rats with carbicron induced a reduction of the phospholipids in both microsomal and plasma membranes. 2. A decrease of the structural order parameter (SDPH) and an increase of the pyrene excimer-to-monomer fluorescence ratio (IE/IM) was also observed, indicating membrane fluidization. 3. The specific activity of membrane-bound phospholipase A2 and phospholipase C were decreased in both types of membranes, whereas acyl-CoA:lysophosphatidylcholine acyltransferase activity was augmented due to carbicron treatment.
Markovska
T
T
Central Laboratory of Biophysics, Bulgarian Academy of Sciences, Sofia.
Koshlukova
S
S
Momchilova
A
A
Pankov
R
R
Yanev
E
E
D008243:1-Acylglycerophosphocholine O-Acyltransferase / Q000378:metabolism; D000818:Animals; D002462:Cell Membrane / Q000187:drug effects* / Q000502:physiology; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D007425:Intracellular Membranes / Q000187:drug effects / Q000502:physiology; D008099:Liver / Q000187:drug effects / Q000648:ultrastructure*; D008297:Male; D008560:Membrane Fluidity; D008862:Microsomes, Liver / Q000648:ultrastructure; D009943:Organophosphorus Compounds / Q000494:pharmacology*; D010741:Phospholipases A / Q000378:metabolism; D054467:Phospholipases A2; D010743:Phospholipids / Q000737:chemistry / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D010738:Type C Phospholipases / Q000378:metabolism
D009943:Organophosphorus Compounds; D010743:Phospholipids; C000944:dicrotophos; D008243:1-Acylglycerophosphocholine O-Acyltransferase; D010741:Phospholipases A; D054467:Phospholipases A2; D010738:Type C Phospholipases