Mass spectrometric investigation of the presence of 7-methyl ring-opened guanine derivatives in urine.
The involvement of chemical alkylating agents in tumorigenesis and chemotherapy is well established and it was shown that one of the main sites of alkylation is the N-7 of guanine in DNA. Though excision of damaged bases is regarded as one of the repair mechanisms in damaged DNA there is a scarcity of information concerning the excised final metabolites in body fluids. This study attempts to demonstrate the usefulness of CAD MS/MS for the detection of the final metabolite-deformylated ring-opened 7 alkylguanine in urine. Such mass spectrometric methods can be used in biomedical studies.
Barak
R
R
Department of Analytical Chemistry, Israel Institute for Biological Research, Ness-Ziona.
Vincze
A
A
Bel
P
P
Dutta
S P
SP
Chedda
G B
GB
D000478:Alkylation; D004249:DNA Damage; D004260:DNA Repair; D008401:Gas Chromatography-Mass Spectrometry; D006147:Guanine / Q000031:analogs & derivatives* / Q000652:urine; D006801:Humans; D013058:Mass Spectrometry; D008745:Methylation
D006147:Guanine; C008450:7-methylguanine