Interleukin-4 production by the malignant cell line HUT78.
Interleukin-4 (IL-4) is a cytokine difficult to induce in large quantities in freshly isolated cells. No malignant cell line has been described to date to produce IL-4 either constitutively or following activation. Here we report that HUT78 cells can be induced to produce IL-4 either by CD3 stimulation under cross-linking conditions or by soluble CD3 mAbs in the presence of PMA. IL-4-specific mRNA could be demonstrated readily under such conditions. Using this experimental model we investigated the role of IL-2 for IL-4 production. The addition of IL-2 strongly costimulated CD3-induced IL-4 production, whereas blockade of the IL-2/IL-2R pathway reduced IL-4 production by approximately 50%. IL-2 alone, however, could not induce IL-4 production. These results indicate that IL-2 is a helper of cytokine necessary, but not sufficient, for IL-4 production in HUT78 cells.
Holter
W
W
Institute of Immunology, University of Vienna, Austria.
Kalthoff
F S
FS
Majdic
O
O
Knapp
W
W
D000911:Antibodies, Monoclonal; D017252:CD3 Complex; D006801:Humans; D007376:Interleukin-2 / Q000096:biosynthesis / Q000494:pharmacology; D015847:Interleukin-4 / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000276:immunology*
D000911:Antibodies, Monoclonal; D017252:CD3 Complex; D007376:Interleukin-2; D012333:RNA, Messenger; D015847:Interleukin-4; D013755:Tetradecanoylphorbol Acetate