A rapid method for measuring serum amyloid A protein by latex agglutination nephelometric immunoassay.
Serum amyloid A protein (SAA) is a sensitive acute phase reactant. We developed a method for the rapid measurement of human SAA in serum by kinetic nephelometry of anti-SAA antibody-coated latex agglutination. Measurement takes less than 6 min using an automated analyser. Standardization of the assay employs SAA-enriched high density lipoprotein as the primary standard. The values determined by our new method and by conventional enzyme-immunoassay showed good agreement (r = 0.988). The normal range was 0.17-10.0 mg/L [mean(SD)]. This rapid method should prove useful in clinical laboratories.
Yamada
T
T
Department of Laboratory Medicine, Niigata University School of Medicine, Niigata, Japan.
Nomata
Y
Y
Sugita
O
O
Okada
M
M
D000911:Antibodies, Monoclonal; D002138:Calibration; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007124:Immunoenzyme Techniques; D007841:Latex Fixation Tests / Q000592:standards; D008075:Lipoproteins, HDL / Q000737:chemistry; D009391:Nephelometry and Turbidimetry / Q000592:standards; D012015:Reference Standards; D012016:Reference Values; D000685:Serum Amyloid A Protein / Q000032:analysis*
D000911:Antibodies, Monoclonal; D008075:Lipoproteins, HDL; D000685:Serum Amyloid A Protein