title: The amino acid sequence of glutathione transferase from Proteus mirabilis, a prototype of a new class of enzymes.
abstract: The complete amino acid sequence of glutathione transferase from Proteus mirabilis was determined. The sequence was reconstructed by analysis of peptides obtained after cleavage by trypsin, Glu-C and Asp-N endoproteinases. The enzyme subunit is composed of 203 amino acid residues corresponding to a molecular mass of 22856 Da. Comparison of this sequence with other known primary structures of the corresponding enzyme from different sources shows a low level of identity (17-26%) with only seven conserved residues in all the sequences considered. This novel glutathione transferase could represent the prototype of a new class, possibly including other bacterial enzymes.
authors.affiliation: Dipartimento di Scienze Biochimiche A. Rossi Fanelli, Università La Sapienza, Roma, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Mignogna
authors.forename: N
authors.initials: N
authors.lastname: Allocati
authors.forename: A
authors.initials: A
authors.lastname: Aceto
authors.forename: R
authors.initials: R
authors.lastname: Piccolomini
authors.forename: C
authors.initials: C
authors.lastname: Di Ilio
authors.forename: D
authors.initials: D
authors.lastname: Barra
authors.forename: F
authors.initials: F
authors.lastname: Martini
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D010450:Endopeptidases / Q000378:metabolism; D005982:Glutathione Transferase / Q000737:chemistry*; D008666:Metalloendopeptidases; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D011513:Proteus mirabilis / Q000201:enzymology*; D017386:Sequence Homology, Amino Acid; D012697:Serine Endopeptidases / Q000378:metabolism; D014357:Trypsin / Q000378:metabolism
chemical_list: D010446:Peptide Fragments; D005982:Glutathione Transferase; D010450:Endopeptidases; D012697:Serine Endopeptidases; C028344:glutamyl endopeptidase; D014357:Trypsin; D008666:Metalloendopeptidases; C058921:endoproteinase Asp-N