Glutathione in whole blood: a novel determination using double quantum coherence transfer proton NMR spectroscopy.
Double quantum selective coherence transfer proton NMR spectroscopy has been used to observe glutathione in whole blood. The efficient water suppression of this technique avoids the need to resuspend the cells in D2O, hence avoiding equilibrium and kinetic isotope effects. Using this method we estimate the concentration of glutathione in fresh whole rabbit blood at approximately 1.7 mM.
Mason
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Department of Radiology, University of Texas Southwestern Medical Center, Dallas 75235-9058.
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Gorrie
G H
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Babcock
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Antich
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D000818:Animals; D002138:Calibration; D005978:Glutathione / Q000097:blood*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D011817:Rabbits; D014867:Water / Q000737:chemistry
D014867:Water; D005978:Glutathione