Comparison of serum copper determination by colorimetric and atomic absorption spectrometric methods in seven different laboratories. The S.F.B.C. (Société Française de Biologie Clinique) Trace Element Group.
An interlaboratory collaborative trial was conducted on the determination of serum copper using two different methods, based on colorimetry (test combination Copper, Boehringer Mannheim, Mannheim, Germany) and flame atomic absorption spectrometry (FAAS). The general performance of the colorimetric method was below that of FAAS, except for sensitivity and linear range, as assessed by detection limit (0.44 versus 1.32 mumol/L) and upper limit of linearity (150 versus 50 mumol/L). The range of the between-run CVs and the recovery of standard additions were, respectively, 2.3-11.9% and 92-127% for the colorimetric method and 1.1-6.0% and 93-101% for the FAAS method. Interferences were minimal with both methods. The two techniques correlated satisfactorily (the correlation coefficients ranged from 0.945-0.970 among laboratories) but the colorimetric assay exhibited slightly higher results than the FAAS method. Each method was transferable among laboratories.
Arnaud
J
J
Laboratoire de Biochimie C, Centre Hospitalier Régional, Grenoble, France.
Chappuis
P
P
Zawislak
R
R
Houot
O
O
Jaudon
M C
MC
Bienvenu
F
F
Bureau
F
F
D000704:Analysis of Variance; D002138:Calibration; D003124:Colorimetry; D003300:Copper / Q000097:blood*; D006801:Humans; D007362:Intensive Care Units; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D013054:Spectrophotometry, Atomic
D011933:Reagent Kits, Diagnostic; D003300:Copper