title: SNAP family of NSF attachment proteins includes a brain-specific isoform.
abstract: The soluble NSF attachment proteins (SNAPs) enable N-ethyl-maleimide-sensitive fusion protein (NSF) to bind to target membranes. Here we report the cloning and sequencing of complementary DNAs encoding alpha-, beta- and gamma-SNAPs. Two of these proteins, alpha and gamma, are found in a wide range of tissues, and act synergistically in intra-Golgi transport. The third, beta, is a brain-specific isoform of alpha-SNAP. Thus, NSF and SNAPs appear to be general components of the intracellular membrane fusion apparatus, and their action at specific sites of fusion must be controlled by SNAP receptors particular to the membranes being fused, as described in the accompanying article.
authors.affiliation: Rockefeller Research Laboratory, Memorial Sloan-Kettering Cancer Center, New York, New York 10021.
authors.forename: S W
authors.initials: SW
authors.lastname: Whiteheart
authors.forename: I C
authors.initials: IC
authors.lastname: Griff
authors.forename: M
authors.initials: M
authors.lastname: Brunner
authors.forename: D O
authors.initials: DO
authors.lastname: Clary
authors.forename: T
authors.initials: T
authors.lastname: Mayer
authors.forename: S A
authors.initials: SA
authors.lastname: Buhrow
authors.forename: J E
authors.initials: JE
authors.lastname: Rothman
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D001923:Brain Chemistry; D002352:Carrier Proteins / Q000737:chemistry*; D002417:Cattle; D003001:Cloning, Molecular; D007425:Intracellular Membranes / Q000737:chemistry; D008561:Membrane Fusion; D051379:Mice; D008969:Molecular Sequence Data; D052066:N-Ethylmaleimide-Sensitive Proteins; D009928:Organ Specificity; D011994:Recombinant Proteins / Q000737:chemistry; D033921:Vesicular Transport Proteins
chemical_list: D002352:Carrier Proteins; D011994:Recombinant Proteins; D033921:Vesicular Transport Proteins; D052066:N-Ethylmaleimide-Sensitive Proteins; C498733:Nsf protein, mouse