Prenatal developmental expression of rat brain 5-HT1A receptor gene followed by PCR.
Expression of the gene encoding the serotonin 5-HT1A receptor was investigated in the brain of rats from embryonic day (ED) 10 to postnatal day 18 using PCR. The 5-HT1A receptor transcripts were first detected as early as ED 12. Their concentration increased to a maximum at ED 15 and decreased progressively to very low levels just before birth (ED 20). In 18-day-old rats, the brain stem levels of 5-HT1A receptor mRNA were higher than at ED 20 but still markedly lower than at ED 15. The high rate of expression of the 5-HT1A receptor gene for a limited period during the fetal life further supports that this receptor might be involved in the trophic action of serotonin during brain maturation.
Hillion
J
J
Laboratoire de Biochimie Cellulaire, CNRS-URA, Collège de France, Paris.
Milne-Edwards
J B
JB
Catelon
J
J
de Vitry
F
F
Gros
F
F
Hamon
M
M
D000367:Age Factors; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000196:embryology* / Q000254:growth & development; D005260:Female; D015870:Gene Expression; D008297:Male; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D011985:Receptors, Serotonin / Q000235:genetics*
D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger; D011985:Receptors, Serotonin