Identification and characterization of tox21A: a mite cDNA encoding a paralytic neurotoxin related to TxP-I.
A cDNA library established from polyadenylated RNA isolated from the predatory mite Pyemotes tritici was screened for cDNAs homologous to tox34, a cDNA encoding an insect-selective paralytic neurotoxin known as TxP-I. Most of the cDNA inserts of homologous clones were shorter or of equal length to tox34 but a few were longer. Further investigation into the nature of these longer clones led to the identification, sequencing and expression of a distinct cDNA referred to as tox21A. This cDNA is predicted to encode a polypeptide which shares approximately 83% amino acid identity with TxP-I. Larvae infected with a recombinant baculovirus expressing tox21A are paralyzed during infection in a manner similar to larvae infected with tox34-expressing recombinants. The tox21A cDNA may represent a duplicated and diverged copy of the TxP-I gene.
Tomalski
M D
MD
Insect Biochemistry Group, Rhone-Poulenc Ag. Co., Research Triangle Park, NC 27709.
Hutchinson
K
K
Todd
J
J
Miller
L K
LK
D000595:Amino Acid Sequence; D000818:Animals; D016367:Baculoviridae / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D007928:Lethal Dose 50; D008925:Mites / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009036:Moths; D009498:Neurotoxins / Q000096:biosynthesis* / Q000633:toxicity; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D011995:Recombination, Genetic
D009498:Neurotoxins; D004247:DNA