Serum levels of tumor necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6), and interleukin-1 beta (IL-1 beta) in dengue-infected patients.
Sensitive immunoenzymatic assays were used to study the levels of tumor necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6), and IL-1 beta in sera from dengue-infected patients obtained during the 1989-1990 outbreak of dengue-3 in Tahiti, French Polynesia. The patients, both children (n = 47) and adults (n = 18), were clinically classified as having dengue hemorrhagic fever (DHF) and graded according to the severity of illness (grade I = fever, grade II = fever with spontaneous hemorrhagic manifestations, grade III = circulatory failure, grade IV = deep shock). The serum samples were obtained from day 1 to day 10 after the onset of the disease. High levels of TNF-alpha were observed in dengue-infected children of all severity grades. The highest values of TNF-alpha were found before day 6 after the onset of the infection, these values decreased from day 6 to day 10. The highest values were observed in sera from grade III and IV patients. High values of IL-6 were observed in serum samples of grade I and II patients on day 1, which decreased on day 4, and by day 5 were similar to those obtained from 25 control children. In grade III and IV patients, the highest values of IL-6 were observed from day 3 to day 5 after the onset of infection; after day 5, these values were very low.(ABSTRACT TRUNCATED AT 250 WORDS)
Hober
D
D
Service Bacteriologie-Virologie B, CHRU, Lille, France.
Poli
L
L
Roblin
B
B
Gestas
P
P
Chungue
E
E
Granic
G
G
Imbert
P
P
Pecarere
J L
JL
Vergez-Pascal
R
R
Wattre
P
P
D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000368:Aged; D002648:Child; D002675:Child, Preschool; D003715:Dengue / Q000097:blood / Q000453:epidemiology / Q000276:immunology*; D004196:Disease Outbreaks; D006801:Humans; D007223:Infant; D007375:Interleukin-1 / Q000097:blood*; D015850:Interleukin-6 / Q000097:blood*; D008875:Middle Aged; D011114:Polynesia / Q000453:epidemiology; D012769:Shock / Q000097:blood / Q000276:immunology; D013577:Syndrome; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis*
D007375:Interleukin-1; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha