Human retinoblastoma cells express alpha B-crystallin in vivo and in vitro.
alpha B-crystallin is a major lens protein that is a member of the heat-shock family of proteins. Using immunohistochemical and northern blot techniques, we now demonstrate its presence in freshly-fixed retinoblastoma tissue. The protein is also abundantly expressed in cultured human retinoblastoma cells (Y-79 NEI, WERI Rb-1) as well as two subcultured Y-79 lines (ATCC and GM01232C). High expression of alpha B-crystallin may be involved in tumor growth and/or be a marker for general oncogenic "stress" in the tumor tissue.
Pineda
R
R
Laboratory of Retinal Cell Biology, National Eye Institute, National Institutes of Health, Bethesda, MD 20892.
Chan
C C
CC
Ni
M
M
Hayden
B J
BJ
Johnson
M A
MA
Nickerson
J
J
Chader
G J
GJ
D000818:Animals; D015152:Blotting, Northern; D002675:Child, Preschool; D003459:Crystallins / Q000096:biosynthesis* / Q000235:genetics; D005134:Eye Neoplasms / Q000378:metabolism* / Q000473:pathology; D005260:Female; D015870:Gene Expression; D006801:Humans; D007124:Immunoenzyme Techniques; D007223:Infant; D007908:Lens, Crystalline / Q000378:metabolism; D008251:Macaca; D051379:Mice; D012333:RNA, Messenger / Q000096:biosynthesis; D012160:Retina / Q000378:metabolism; D012175:Retinoblastoma / Q000378:metabolism* / Q000473:pathology; D014407:Tumor Cells, Cultured
D003459:Crystallins; D012333:RNA, Messenger