Endothelin-1 stimulated phospholipase D in A10 vascular smooth muscle derived cells is dependent on tyrosine kinase. Evidence for involvement in stimulation of mitogenesis.
The mechanism whereby endothelin stimulates mitogenesis of vascular smooth muscle cells is not understood. Here we show that endothelin-1 stimulates phospholipase D by a protein kinase C and tyrosine kinase dependent mechanism, and present evidence that implicate the phosphatidic acid formed by phospholipase D in the mitogenic response.
Wilkes
L C
LC
Department of Pharmacology and Therapeutics, University of Leicester, UK.
Patel
V
V
Purkiss
J R
JR
Boarder
M R
MR
D000818:Animals; D002455:Cell Division / Q000502:physiology; D002478:Cells, Cultured; D016232:Endothelins / Q000502:physiology*; D009131:Muscle, Smooth, Vascular / Q000201:enzymology*; D010739:Phospholipase D / Q000378:metabolism*; D011493:Protein Kinase C / Q000502:physiology; D011505:Protein-Tyrosine Kinases / Q000502:physiology*; D051381:Rats; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
D016232:Endothelins; D014316:Tritium; D011505:Protein-Tyrosine Kinases; D011493:Protein Kinase C; D010739:Phospholipase D; D013936:Thymidine