title: The effect of intrinsic curvature on supercoiling: predictions of elasticity theory.
abstract: Elasticity theory of naturally curved rods is employed to study the effects of intrinsic curvature on the properties of the equilibrium conformations of supercoiled DNA. The results stand in sharp contrast to those obtained when the molecule is viewed as being straight in its relaxed form. Starting from very fundamental principles of the theory, we show that the torsion of an open segment with a curved duplex axis can vary when the temperature, and along with it, the intrinsic twist is changed. Conversely, an imposed helicity, such as might be associated with binding to a histone, can change the intrinsic twist. It is also shown that another consequence of the presence of naturally curved sequences is that the twist density will, in general, vary with position along the chain in all equilibrium states. Then portions of the molecule will be more or less susceptible to interaction with other agents sensitive to such a variation. Finally, some closed equilibrium global structures uniquely associated with intrinsic curvature are discussed.
authors: Department of Chemistry, Rutgers, State University of New Jersey, New Brunswick 08903.
authors: I
authors: I
authors: Tobias
authors: W K
authors: WK
authors: Olson
mesh_terms: D004278:DNA, Superhelical / Q000737:chemistry*; D004548:Elasticity; D006657:Histones / Q000737:chemistry; D008433:Mathematics; D008956:Models, Chemical; D009690:Nucleic Acid Conformation; D013816:Thermodynamics
chemical_list: D004278:DNA, Superhelical; D006657:Histones