title: Lymphocytes bearing the gamma delta T cell receptor in acute Brucella melitensis infection.
abstract: A phenotypical analysis carried out by indirect immunofluorescence and two-color cytofluorometry showed that the number of lymphocytes bearing the gamma delta T cell receptor (TcR) heterodimer was dramatically increased in the blood of six children with Brucella melitensis infection. Most in vivo expanded gamma delta T cells reacted with a monoclonal antibody which identifies V delta 2 gene products and a significant proportion expressed CD25 and HLA-DR activation antigens. In addition, whereas only a few gamma delta T lymphocytes were CD8+, nearly all were CD4-. Highly enriched populations of both alpha beta and gamma delta T cells were obtained by negative immunoselection from three subjects with brucellosis sampled during convalescence. Despite the different form of their TcR, the proliferation of these two major T cell subsets in response to a mitogenic anti-CD3 monoclonal reagent (OKT3) was optimal. In contrast, alpha beta, but not gamma delta, T lymphocytes proliferated vigorously in response to the antigenic stimulus elicited by heat-killed Brucella. Further studies are, therefore, needed to determine whether the selective expansion of the gamma delta T cell subpopulation observed during the clinical course of the infection is driven by antigenic determinant(s) borne by the pathogen in vivo or is due to host-derived stimuli, such as autologous heat-shock proteins expressed on the surface of the infected cells.
authors: Department of Pediatrics, Perugia University Medical School, Italy.
authors: A
authors: A
authors: Bertotto
authors: R
authors: R
authors: Gerli
authors: F
authors: F
authors: Spinozzi
authors: C
authors: C
authors: Muscat
authors: F
authors: F
authors: Scalise
authors: G
authors: G
authors: Castellucci
authors: M
authors: M
authors: Sposito
authors: F
authors: F
authors: Candio
authors: R
authors: R
authors: Vaccaro
mesh_terms: D000208:Acute Disease; D000293:Adolescent; D017347:Brucella melitensis; D002006:Brucellosis / Q000276:immunology*; D002648:Child; D006801:Humans; D008213:Lymphocyte Activation; D010641:Phenotype; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000032:analysis; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000032:analysis*; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D016693:Receptors, Antigen, T-Cell, alpha-beta; D016692:Receptors, Antigen, T-Cell, gamma-delta