title: Serial quantitation of hyaluronan and sulfated glycosaminoglycans in fetal sheep skin.
abstract: Fetal wounds are abundant in hyaluronic acid (HA), but little is known as to the total HA content of fetal tissues as a function of gestational age. Previous studies demonstrated scarless healing prior to approximately 130 days gestation, after which disorganized collagen deposition became prevalent. Glycosaminoglycans (GAGs) have been shown to play an important role in wound healing, cytodifferentiation, and morphogenesis. In this study, we examined the HA and GAG content of fetal sheep skin of increasing gestational age. We found that the total amount of HA and GAGs declined from a high at 80 days gestation (528 +/- 9 micrograms/gm) to a low at 130 days (174 +/- 11). Analysis of the various GAG species revealed that HA comprised the largest fraction (75-96%). The sulfated GAGs, Heparan Sulfate (HS) and Dermatan Sulfate (DS), were not present in the extracellular matrix until 120 days gestation. Both the trough of HA content and the appearance of sulfated GAGs in the extracellular fraction correspond to the appearance of scarring in fetal sheep wound repair.
authors: Institute of Reconstructive Plastic Surgery, New York University Medical Center, NY 10016.
authors: R M
authors: RM
authors: Freund
authors: J W
authors: JW
authors: Siebert
authors: R C
authors: RC
authors: Cabrera
authors: M T
authors: MT
authors: Longaker
authors: Y
authors: Y
authors: Eidelman
authors: N S
authors: NS
authors: Adzick
authors: H G
authors: HG
authors: Garg
mesh_terms: D000818:Animals; D004586:Electrophoresis; D005333:Fetus / Q000737:chemistry*; D005865:Gestational Age; D006025:Glycosaminoglycans / Q000032:analysis*; D006820:Hyaluronic Acid / Q000032:analysis*; D012756:Sheep; D012867:Skin / Q000737:chemistry / Q000196:embryology*; D013431:Sulfates
chemical_list: D006025:Glycosaminoglycans; D013431:Sulfates; D006820:Hyaluronic Acid