Ultrastructural and immunocytochemical study of P1 protamine localization in human testis.
Two monoclonal antibodies (MAbs) directed against human protamine P1 were realized. Anti-P1 specificity was assessed by western-blot and confirmed by ELISA. Monoclonal antibody 97-3 was selected. Protamine P1 was specifically demonstrated in human testis by immunoelectron microscopy, using 97-3 MAb and an indirect post-embedding immunogold technique. Our results clearly demonstrated the precise time of appearance of P1 protamine in the nuclei of human spermatids. P1 first appeared in the nucleus of step 5 spermatids and its concentration was increased in steps 6-8 spermatids, cytoplasm was not labelled.
Lescoat
D
D
Laboratoire d'Histologie et d'Embryologie, Rennes, France.
Blanchard
Y
Y
Lavault
M T
MT
Quernée
D
D
Le Lannou
D
D
D000911:Antibodies, Monoclonal; D015153:Blotting, Western; D002467:Cell Nucleus / Q000737:chemistry; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D016253:Microscopy, Immunoelectron; D011479:Protamines / Q000032:analysis*; D013087:Spermatids / Q000737:chemistry / Q000648:ultrastructure; D013737:Testis / Q000737:chemistry* / Q000648:ultrastructure*
D000911:Antibodies, Monoclonal; D011479:Protamines; C046533:protamine P1