Dietary vitamin E reverses the effects of ultraviolet light irradiation on rat skin glycosaminoglycans.
Chondroitin 4-sulfate (Ch 4-S), three dermatan sulfates (DS18, DS45a, DS45b) and hyaluronic acid (HA) were the major glycosaminoglycans (GAG) isolated from the skin of 4 groups of albino rats. The yields from Group 1 (control) were: Ch 4-S, 0.015%; HA, 0.028% and DS (total), 0.098% (w/w). Traces of heparin were detected only in rats irradiated with ultraviolet (UV) light (Group II), in the GAG pool isolated with 45% ethanol. Yields increased by at least 28% (w/w) in Group II, but decreased, except HA's, also by at least 28%, below the level of the control, in irradiated rats that also ingested vitamin E (Group III). The sulfate composition of these GAG determined by infrared spectroscopy was as follows: approx. 17% (w/w) for DS18, 21-30% for DS45a, 21-35% for DS45b and 26-44% for Ch 4-S. A 60-70% (mol/mol) N-acetylation of hexosamine in DS45 was estimated by Fourier transform 1H-NMR spectroscopy; the IdUA composition of this DS was 30-46% (mol/mol), and the uronic acid/hexosamine ratio ranged from 2.50:1 to 1.6:1. The data show UV light irradiation of rat skin to result in an abnormally elevated production of the major GAG and oversulfation of Ch 4-S and DS. These effects are reversed, except for the sulfation of DS45b, when the irradiated animals also ingest vitamin E.
Longas
M O
MO
Purdue University Calumet, Department of Chemistry and Physics, Hammond, IN 46323-2094.
Bhuyan
D K
DK
Bhuyan
K C
KC
Gutsch
C M
CM
Breitweiser
K O
KO
D000818:Animals; D002809:Chondroitin Sulfates / Q000378:metabolism / Q000528:radiation effects; D003871:Dermatan Sulfate / Q000378:metabolism / Q000528:radiation effects; D004032:Diet; D006025:Glycosaminoglycans / Q000378:metabolism / Q000528:radiation effects*; D006820:Hyaluronic Acid / Q000378:metabolism / Q000528:radiation effects; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000528:radiation effects*; D014466:Ultraviolet Rays; D014810:Vitamin E / Q000494:pharmacology*
D006025:Glycosaminoglycans; D011837:Radiation-Protective Agents; D014810:Vitamin E; D003871:Dermatan Sulfate; D006820:Hyaluronic Acid; D002809:Chondroitin Sulfates