Expression and secretion of Bacillus amyloliquefaciens alpha-amylase by using the yeast pheromone alpha-factor promoter and leader sequence in Saccharomyces cerevisiae.
Replacement of the regulatory and secretory signals of the alpha-amylase gene (AMY) from Bacillus amylolique-faciens with the complete yeast pheromone alpha-factor prepro region (MF alpha 1p) resulted in increased levels of extracellular alpha-amylase production in Saccharomyces cerevisiae. However, the removal of the (Glu-Ala)2 peptide from the MF alpha 1 spacer region (Lys-Arg-Glu-Ala-Glu-Ala) yielded decreased levels of extracellular alpha-amylase.
Southgate
V J
VJ
Department of Microbiology, University of Stellenbosch, South Africa.
Steyn
A J
AJ
Pretorius
I S
IS
Van Vuuren
H J
HJ
D001407:Bacillus / Q000201:enzymology* / Q000235:genetics; D001483:Base Sequence; D015971:Gene Expression Regulation, Enzymologic; D000072235:Mating Factor; D008969:Molecular Sequence Data; D010455:Peptides / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics; D014158:Transcription, Genetic; D014162:Transfection; D000516:alpha-Amylases / Q000235:genetics / Q000378:metabolism*
D010455:Peptides; D000072235:Mating Factor; D000516:alpha-Amylases