title: Conversion of xanthine dehydrogenase to xanthine oxidase during ischemia of the rat small intestine and the effect of trifluoperazine on the conversion.
abstract: The conversion from xanthine dehydrogenase (XD) to xanthine oxidase (XO) and the effect of trifluoperazine (TFP), a calmodulin inhibitor, on the conversion were examined during the normothermic ischemia of the rat small intestine. Rat jejunums were stored in lactated Ringer's solution (LR) at 37 degrees C for various hours after intravascular flushing with LR. The extents of the conversion from XD to XO (%XO) constituted 21.1% +/- 3.0%, 36.2% +/- 7.0%, 63.2% +/- 8.1%, and 88.2% +/- 8.6% after 0, 2, 4, and 6 hours of the preservation, respectively (control group). The preservation without the intravascular flushing showed significant increase in the %XO (99.5% +/- 6.0%) only after 6 hours compared with those in the control group (P < .05). When the intestines were stored in LR containing 50 mg/L of TFP at 37 degrees C, or stored in LR at 37 degrees C after the intraperitoneal pretreatment with 10 mg/kg of TFP 1 hour before laparotomy showed significant decrease in the extents of the conversion after 4 hours (P < .005) and 6 hours (P < .025) of the preservation, compared with those in the control group. When the dose of TFP for the pretreatment was increased to 50 mg/kg, the suppressive effect on the conversion was found even after 2 hours (P < .025) as well as after 4 hours (P < .005) and 6 hours (P < .025) of the preservation. These results suggest that TFP could be effective on reducing the XO-mediated postischemic reperfusion injury by means of inhibiting the conversion during ischemia of the rat small intestine.
authors: Department of Pediatric Surgery, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
authors: Y
authors: Y
authors: Hirata
authors: K
authors: K
authors: Ishii
authors: T
authors: T
authors: Taguchi
authors: S
authors: S
authors: Suita
authors: K
authors: K
authors: Takeshige
mesh_terms: D000818:Animals; D066298:In Vitro Techniques; D007511:Ischemia / Q000201:enzymology*; D007583:Jejunum / Q000098:blood supply* / Q000201:enzymology*; D008297:Male; D051381:Rats; D015427:Reperfusion Injury / Q000201:enzymology; D014268:Trifluoperazine / Q000494:pharmacology*; D014968:Xanthine Dehydrogenase / Q000378:metabolism*; D014969:Xanthine Oxidase / Q000378:metabolism*
chemical_list: D014268:Trifluoperazine; D014968:Xanthine Dehydrogenase; D014969:Xanthine Oxidase