Different TRE-related elements are distinguished by sets of DNA-binding proteins with overlapping sequence specificity.
Several promoter elements with sequence similarity to the prototype TPA-responsive element (TRE) were compared by mobility-shift analyses. Activities within whole cell extracts were identified that bind to the TRE-like elements in the collagenase, the somatostatin, and the c-jun promoters. The corresponding factors appeared to differ in their degree of selectivity for these TRE-like sequences. One protein species bound equally well to all TREs. In addition, a subset of specific activities recognised only the somatostatin and the c-jun-derived element and one DNA-protein complex had exclusive specificity for the TRE present in the c-jun promoter. By antibody 'supershift' assays some of the protein components of the specific complexes were identified as CREB- and ATF-related products. Based on these data we postulate that bZip protein dimers differ in their ability to tolerate variations from the canonical TRE sequence. We propose that TRE-like promoter elements are distinguished by this ability to bind to different subsets of a family of related transcription factors.
Smith
S E
SE
European Molecular Biology Laboratory, Heidelberg, Germany.
Papavassiliou
A G
AG
Bohmann
D
D
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D017364:Collagenases / Q000235:genetics; D016384:Consensus Sequence; D017362:Cyclic AMP Response Element-Binding Protein / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D010450:Endopeptidases; D016758:Genes, jun; D008907:Mink; D008969:Molecular Sequence Data; D015513:Oncogene Proteins; D015514:Oncogene Proteins, Fusion / Q000378:metabolism*; D011401:Promoter Regions, Genetic / Q000235:genetics; D011485:Protein Binding; D011518:Proto-Oncogene Proteins; D012045:Regulatory Sequences, Nucleic Acid / Q000502:physiology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D043222:Ubiquitin Thiolesterase
D017362:Cyclic AMP Response Element-Binding Protein; D004268:DNA-Binding Proteins; D015513:Oncogene Proteins; D015514:Oncogene Proteins, Fusion; D011518:Proto-Oncogene Proteins; D010450:Endopeptidases; C473330:USP6 protein, human; D043222:Ubiquitin Thiolesterase; D017364:Collagenases; D013755:Tetradecanoylphorbol Acetate