Human neuroblastoma cells produce the NF-kappa B-like HIV-1 transcription activator during differentiation.
Human neuroblastoma GOTO and IMR32 cells differentiate into neural cells on treatment with 5-BrdU. Here we report that in association with differentiation induced by 5-BrdU, the transcription of HIV-1 was activated in both neuroblastoma cell lines. An NF-kappa B-like factor was also found to be produced in association with the neural cell differentiation of these cells. These results suggest that, during differentiation of neural cells, an NF-kappa B-like factor was produced, and that when the neural cells are infected with HIV-1 the production of NF-kappa B results in activation of transcription of HIV-1. Thus this NF-kappa B-like factor may be involved in the mechanism causing AIDS dementia.
Kurata
S
S
Medical Research Institute, Tokyo Medical and Dental University, Japan.
Wakabayashi
T
T
Ito
Y
Y
Miwa
N
N
Ueno
R
R
Marunouchi
T
T
Kurata
N
N
D001483:Base Sequence; D001665:Binding Sites; D001973:Bromodeoxyuridine / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology*; D002455:Cell Division / Q000187:drug effects; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics / Q000378:metabolism; D016325:HIV Long Terminal Repeat; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D007700:Kinetics; D008969:Molecular Sequence Data; D016328:NF-kappa B / Q000096:biosynthesis*; D009447:Neuroblastoma; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D009687:Nuclear Proteins / Q000378:metabolism*; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000378:metabolism; D015183:Restriction Mapping; D013379:Substrate Specificity; D016385:TATA Box; D015534:Trans-Activators / Q000096:biosynthesis*; D014158:Transcription, Genetic / Q000187:drug effects; D015533:Transcriptional Activation / Q000187:drug effects; D014162:Transfection; D014407:Tumor Cells, Cultured
D016328:NF-kappa B; D009687:Nuclear Proteins; D009838:Oligodeoxyribonucleotides; D015534:Trans-Activators; D015500:Chloramphenicol O-Acetyltransferase; D001973:Bromodeoxyuridine