A quantitative ELISA for dystrophin.
A novel approach to the quantitation of the muscular dystrophy protein, dystrophin, in muscle extracts is described. The two-site ELISA uses two monoclonal antibodies against dystrophin epitopes which lie close together in the rod domain of the dystrophin molecule in order to minimize the effects of dystrophin degradation. Dystrophin is assayed in its native form by extracting with non-ionic detergents and avoiding the use of SDS.
Morris
G E
GE
Research Division, N.E. Wales Institute, Deeside, Clwyd, UK.
Ellis
J M
JM
Nguyen
T M
TM
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D016189:Dystrophin / Q000032:analysis* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D066298:In Vitro Techniques; D051379:Mice; D009132:Muscles / Q000737:chemistry*; D009136:Muscular Dystrophies / Q000175:diagnosis; D009137:Muscular Dystrophy, Animal / Q000175:diagnosis
D000911:Antibodies, Monoclonal; D016189:Dystrophin