Development of an ocular irritation test in the mouse: image analysis of corneal lesions.
An ocular irritation test in the mouse has been developed, in which corneal lesions consecutive to instillation of ophthalmic preparations are assessed by digital image processing. The high sensitivity of this method makes it possible to test very low (i.e. therapeutic) concentrations, which do not produce macroscopic damage to the eye. The relatively low variability of the results allows the determination of irritation potential with a small number of animals. The test permits the establishment of the relationship between instilled concentrations and corneal lesions. The rapidity and simplicity of this approach make the proposed test suitable for the screening of ophthalmic preparations.
Magada
M
M
Ecole de Pharmacie, Institut de Pharmacie galénique et de Biopharmacie, Université de Lausanne, Switzerland.
Mayer
J M
JM
Etter
J C
JC
D000818:Animals; D001548:Benzalkonium Compounds / Q000633:toxicity*; D003316:Corneal Diseases / Q000139:chemically induced* / Q000473:pathology; D007091:Image Processing, Computer-Assisted; D051379:Mice; D009883:Ophthalmic Solutions / Q000633:toxicity*; D012967:Sodium Dodecyl Sulfate / Q000633:toxicity*; D013748:Tetracaine / Q000633:toxicity*; D014741:Video Recording
D001548:Benzalkonium Compounds; D009883:Ophthalmic Solutions; D013748:Tetracaine; D012967:Sodium Dodecyl Sulfate