Achiral and chiral high-performance liquid chromatographic determination of tramadol and its major metabolites in urine after oral administration of racemic tramadol.
A reversed-phase high-performance liquid chromatographic method for the simultaneous determination of tramadol and its major metabolites O-demethyltramadol and N-demethyltramadol in urine has been developed. The determination of the enantiomeric ratios of the three compounds was achieved using a Chiralpak AD column and a Chiralcel OD column, respectively. After oral administration of racemic tramadol to five healthy volunteers, inter-individual differences of the excreted amounts and the enantiomeric ratios of the compounds were observed.
Elsing
B
B
Department of Pharmaceutical Chemistry, University of Münster, Germany.
Blaschke
G
G
D000284:Administration, Oral; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D013237:Stereoisomerism; D014147:Tramadol / Q000031:analogs & derivatives / Q000493:pharmacokinetics / Q000652:urine*
C080580:O-demethyltramadol; D014147:Tramadol; C080579:N-demethyltramadol