title: Properties of Azotobacter vinelandii rhodanese.
abstract: Rhodanese (thiosulfate: cyanide sulfurtransferase, E.C. 2.8.1.1) was purified from the nitrogen fixing organism Azotobacter vinelandii, and its amino acid composition was determined. The enzyme is a single polypeptide chain of M(r) 29,000 which showed an apparent pI of 4.7 and no presence of isoenzymes. Steady-state initial velocity measurements indicate that the enzyme catalyzes the transfer of sulfane sulfur of thiosulfate either to cyanide or to dihydrolipoate by way of a double displacement mechanism.
authors: Dipartimento di Scienze Molecolari Agroalimentari, Università di Milano, Italy.
authors: S
authors: S
authors: Pagani
authors: G
authors: G
authors: Sessa
authors: F
authors: F
authors: Sessa
authors: R
authors: R
authors: Colnaghi
mesh_terms: D000596:Amino Acids / Q000032:analysis*; D016948:Azotobacter vinelandii / Q000201:enzymology*; D002851:Chromatography, High Pressure Liquid; D003486:Cyanides / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D007700:Kinetics; D008970:Molecular Weight; D008063:Thioctic Acid / Q000031:analogs & derivatives / Q000378:metabolism; D013884:Thiosulfate Sulfurtransferase / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D013885:Thiosulfates / Q000378:metabolism
chemical_list: D000596:Amino Acids; D003486:Cyanides; D013885:Thiosulfates; D008063:Thioctic Acid; C015144:dihydrolipoic acid; D013884:Thiosulfate Sulfurtransferase