The order and orientation of a cluster of metalloproteinase genes, stromelysin 2, collagenase, and stromelysin, together with D11S385, on chromosome 11q22-q23.
A cluster of metalloproteinase genes, stromelysin, fibroblast collagenase, and stromelysin 2 together with the anonymous DNA marker D11S385, was mapped using pulsed-field gel electrophoresis to a 135-kb region of chromosome 11q22-q23. The physical proximity of these markers was subsequently confirmed using two YAC clones, and their relative order was established as stromelysin 2-collagenase-stromelysin-D11S385. The pattern of marker representation in a panel of radiation-reduced chromosome 11 hybrids suggests that the metalloproteinase gene/D11S385 cluster is orientated with STMY2 closest to the centromere.
Formstone
C J
CJ
Department of Cancer Studies, Medical School, University of Birmingham, United Kingdom.
Byrd
P J
PJ
Ambrose
H J
HJ
Riley
J H
JH
Hernandez
D
D
McConville
C M
CM
Taylor
A M
AM
D000818:Animals; D001483:Base Sequence; D002880:Chromosomes, Human, Pair 11; D017364:Collagenases / Q000235:genetics; D006224:Cricetinae; D004247:DNA / Q000235:genetics; D015342:DNA Probes; D005819:Genetic Markers; D006023:Glycoproteins / Q000235:genetics; D006801:Humans; D006822:Hybrid Cells; D053506:Matrix Metalloproteinase 10; D019278:Matrix Metalloproteinase 3; D008666:Metalloendopeptidases / Q000235:genetics*; D008969:Molecular Sequence Data; D005810:Multigene Family
D015342:DNA Probes; D005819:Genetic Markers; D006023:Glycoproteins; D004247:DNA; D017364:Collagenases; D008666:Metalloendopeptidases; D019278:Matrix Metalloproteinase 3; D053506:Matrix Metalloproteinase 10