title: Pharmacokinetics of cytotoxin from Chinese cobra (Naja naja atra) venom.
abstract: The pharmacokinetics of cytotoxin from Chinese cobra (Naja naja atra) venom was studied in rabbits after i.v. and i.m. injection of 0.2 and 0.5 mg.kg-1, respectively. The plasma levels of the cytotoxin were analysed by a biotin-avidin enzyme-linked immunosorbent assay. The plasma concentration-time course after i.v. administration fitted a two-compartment open model. The half-life (mean +/- S.D.) of the alpha-phase was 5.8 +/- 0.6 min and that of the beta-phase 3.5 +/- 0.2 hr. Apparent volume of distribution was 1.7 +/- 0.3 litres.kg-1, and clearance was 5.6 +/- 1.4 ml.min-1. A rapid absorption was observed after i.m. injection with peak plasma level of 260 +/- 90 ng.ml-1 reached within 13.6 +/- 2.4 min. The absorption rate constant was 0.16 +/- 0.03 ml-1. The area under the plasma concentration-time curve was 33 +/- 15 micrograms.min.ml-1.
authors: Laboratory of Snake Venom Research, Fujian Medical College, Fuzhou, P.R. China.
authors: M P
authors: MP
authors: Guo
authors: Q C
authors: QC
authors: Wang
authors: G F
authors: GF
authors: Liu
mesh_terms: D000818:Animals; D001360:Avidin; D001710:Biotin; D003603:Cytotoxins / Q000008:administration & dosage / Q000493:pharmacokinetics* / Q000633:toxicity; D004546:Elapid Venoms / Q000493:pharmacokinetics* / Q000633:toxicity; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006207:Half-Life; D007273:Injections, Intramuscular; D007275:Injections, Intravenous; D007457:Iodine Radioisotopes; D007928:Lethal Dose 50; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011817:Rabbits
chemical_list: D003603:Cytotoxins; D004546:Elapid Venoms; D007457:Iodine Radioisotopes; D001360:Avidin; D001710:Biotin