Alterations in lipid characteristics of lysosomes are involved in liver necrosis induced by thioacetamide.
To determine whether lysosomal lipid composition is altered in hepatic necrosis, we studied this parameter in thioacetamide-induced necrosis and in its regenerating stage as well as in the recovery of thioacetamide-induced injury. Results showed that in liver necrosis there is an increase in the protein and phospholipid lysosomal contents. This effect may be related to an increased number of lysosomes. These organelles also suffered a decrease in cholesterol content. When liver necrosis was recovered either pharmacologically or spontaneously all three parameters recovered their normal values. These results suggest that lysosomes and their lipid composition play a role in progression of hepatic necrosis.
Osada
J
J
Instituto de Bioquímica, Facultad de Farmacia, Universidad Complutense, Madrid, Spain.
Aylagas
H
H
Miró-Obradors
M J
MJ
Palacios-Alaiz
E
E
D000135:Acid Phosphatase / Q000378:metabolism; D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D056486:Chemical and Drug Induced Liver Injury / Q000378:metabolism*; D002784:Cholesterol / Q000378:metabolism; D050356:Lipid Metabolism; D008247:Lysosomes / Q000378:metabolism*; D008297:Male; D008566:Membranes / Q000187:drug effects / Q000378:metabolism; D009336:Necrosis / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012436:S-Adenosylmethionine / Q000494:pharmacology; D013853:Thioacetamide / Q000633:toxicity*
D013853:Thioacetamide; D012436:S-Adenosylmethionine; D002784:Cholesterol; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D000135:Acid Phosphatase