title: Histone H1 deposition and histone-DNA interactions in replicating chromatin.
abstract: An immunochemical method for analyzing protein interactions with BrdUrd-substituted DNA was used to study binding of histones to nascent DNA in nuclei. The results indicate that in Ehrlich ascites tumor (EAT) cells, histone H1 deposits on newly replicated DNA simultaneously with or immediately after core histone deposition so that in chromatin replicated for 3 min, the stoichiometry of the histones is the same as in bulk chromatin. All histones, and especially histone H1, interact with nascent DNA more weakly than with bulk chromatin, although the efficiency of interaction via the globular domains seems to be the same for both types of chromatin.
authors: Institute of Molecular Biology, Russian Academy of Sciences, Moscow.
authors: S
authors: S
authors: Bavykin
authors: L
authors: L
authors: Srebreva
authors: T
authors: T
authors: Banchev
authors: R
authors: R
authors: Tsanev
authors: J
authors: J
authors: Zlatanova
authors: A
authors: A
authors: Mirzabekov
mesh_terms: D000818:Animals; D001973:Bromodeoxyuridine; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism*; D002458:Cell Fractionation; D002467:Cell Nucleus / Q000378:metabolism / Q000648:ultrastructure; D002843:Chromatin / Q000378:metabolism* / Q000502:physiology; D003432:Cross-Linking Reagents; D004261:DNA Replication; D004273:DNA, Neoplasm / Q000302:isolation & purification / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D006657:Histones / Q000302:isolation & purification / Q000378:metabolism*; D051379:Mice; D013463:Sulfuric Acid Esters; D014407:Tumor Cells, Cultured
chemical_list: D002843:Chromatin; D003432:Cross-Linking Reagents; D004273:DNA, Neoplasm; D006657:Histones; D013463:Sulfuric Acid Esters; D001973:Bromodeoxyuridine; C007482:dimethyl sulfate