A monoclonal antibody to Shigella dysenteriae serotype 13 cross-reacting with Shiga toxin.
A monoclonal antibody (mAb ICT6) was produced against the newly described Shigella dysenteriae serotype type 13. The mAb was of IgM isotype and recognized purified Shiga toxin in ELISA and immunoblot. It also recognized periplasmic extract S. dysenteriae type 13 in immunoblot as did an affinity-purified polyclonal rabbit antiserum and a previously described monoclonal antibody to the B subunit of Shiga toxin. The mAb ICT6 did not neutralize the cytotoxic effects or S. dysenteriae type 13, Shiga toxin or periplasmic extracts of S. dysenteriae type 1 for HeLa cells.
Qadri
F
F
International Centre for Diarrhoeal Disease Research, Bangladesh, (ICDDR,B), Dhaka.
Azim
T
T
Hossain
A
A
Islam
D
D
Mondol
G
G
Faruque
S M
SM
Albert
M J
MJ
D000818:Animals; D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D001427:Bacterial Toxins / Q000276:immunology; D003429:Cross Reactions; D006367:HeLa Cells; D006801:Humans; D009500:Neutralization Tests; D012703:Serotyping; D022603:Shiga Toxins; D012762:Shigella dysenteriae / Q000145:classification / Q000276:immunology*
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D001427:Bacterial Toxins; D022603:Shiga Toxins