title: Incorporation of D3H glucosamine to the adult and developing cochlear tectorial membrane of normal and hypothyroid rats.
abstract: The uptake of D-3H-glucosamine by the developing cochlea of normal and hypothyroid rats was examined using light microscopic radioautography. During postnatal development, normal and hypothyroid rat cochleas exhibited a layer of radiolabelling in the tectorial membrane (TM). This layer first appeared in the TM region which covers the spiral limbus and the Kölliker's organ (KO), then progressively reached the apical part of the TM covering the organ of Corti. Radiolabelling was significantly greater in hypothyroid than in normal cochleas. These findings suggests that the enormous size reached by the TM in the congenital hypothyroidism could be related to an increase of epithelial secretion, at least for carbohydrates. It also suggests that TM, in normal and hypothyroid cochleas, could be formed during development by the addition of successive layers. Older layers could be displaced upwards by the new ones. Cochleas of normal young adult rats, treated with D-3H-glucosamine, showed a very scarce and diffuse radiolabelling. Cochleas of hypothyroid young adult rats exhibited a thickened and distorted TM, which incorporated a significant amount of carbohydrates. These results suggest that TM secretion is highly reduced in young adult normal animals, while in young adult hypothyroid ones it is still active. During cochlear maturation, thyroxine seems to be necessary, not only for the synthesis of normal glycoproteins (as suggested by previous reports), but also for the control of glycoprotein secretion.
authors: Departamento de Ciencias Morfológicas, Facultad de Medicina, Universidad Complutense, Madrid, Spain.
authors: M
authors: M
authors: Remezal
authors: P
authors: P
authors: Gil-Loyzaga
mesh_terms: D000818:Animals; D001345:Autoradiography; D003051:Cochlea / Q000166:cytology / Q000254:growth & development / Q000378:metabolism* / Q000473:pathology; D005944:Glucosamine / Q000378:metabolism* / Q000494:pharmacology; D007037:Hypothyroidism / Q000378:metabolism* / Q000473:pathology; D009925:Organ of Corti / Q000378:metabolism; D051381:Rats; D013680:Tectorial Membrane / Q000166:cytology / Q000254:growth & development / Q000378:metabolism* / Q000473:pathology
chemical_list: D005944:Glucosamine