Effect of CDP-choline treatment on mitochondrial and synaptosomal protein composition in different brain regions during aging.
Several age-dependent modifications of inner mitochondrial membrane and synaptosomal plasma membrane proteins from different brain regions of 4-, 12-, 18- and 24-month-old male Wistar rats, were observed. Some proteins, identified by immunoblotting assay as various subunits of mitochondrial respiratory chain complexes and calmodulin, were particularly impaired. Chronic treatment with CDP-choline at a dose of 20 mg/kg body weight per day for 28 days caused significant changes in the amounts of several of the above mentioned proteins. Most of the proteins, which decreased during aging, showed a significant increase after CDP-choline treatment compared with the corresponding control values at the same age. The effect of CDP-choline might be due to: the increased availability of cytidylic nucleotides, which in the brain are present in limited amounts compared to the other nucleotides; the increased content of total adenine nucleotides; the improvement of brain energy metabolism.
Villa
R F
RF
Institute of Pharmacology, Faculty of Science, University of Pavia, Italy.
Ingrao
F
F
Magri
G
G
Gorini
A
A
Reale
S
S
Costa
A
A
Ragusa
N
N
Avola
R
R
Giuffrida-Stella
A M
AM
D000375:Aging / Q000378:metabolism*; D000818:Animals; D001923:Brain Chemistry / Q000187:drug effects*; D002462:Cell Membrane / Q000378:metabolism; D003566:Cytidine Diphosphate Choline / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D002534:Hypoxia, Brain / Q000378:metabolism; D015151:Immunoblotting; D008297:Male; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013574:Synaptosomes / Q000187:drug effects / Q000378:metabolism*
D009419:Nerve Tissue Proteins; D003566:Cytidine Diphosphate Choline