title: Gene A32 product of vaccinia virus may be an ATPase involved in viral DNA packaging as indicated by sequence comparisons with other putative viral ATPases.
abstract: Statistically significant sequence similarity was revealed between the gene A32 product of vaccinia virus (VV), gene I products (gpI) of filamentous single-stranded DNA bacteriophages, and IVa2 gene products of adenoviruses. Four conserved sequence motifs were delineated, the two N-proximal of which correspond to the A and B motifs of the purine NTP-binding pattern. Based on the role of gpI and IVa2 proteins in virion morphogenesis, and on the conservation of the NTP-binding pattern in these proteins, we hypothesize that the A32 gene product might be involved in an ATP-consuming function in VV virion formation, e.g., packaging of the DNA in the virus particle.
authors: Institute of Microbiology, Academy of Medical Sciences, Moscow, Russia.
authors: E V
authors: EV
authors: Koonin
authors: T G
authors: TG
authors: Senkevich
authors: V I
authors: VI
authors: Chernos
mesh_terms: D000251:Adenosine Triphosphatases / Q000235:genetics*; D000256:Adenoviridae / Q000235:genetics; D000595:Amino Acid Sequence; D001435:Bacteriophages / Q000235:genetics; D004279:DNA, Viral / Q000378:metabolism; D005814:Genes, Viral; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D014616:Vaccinia virus / Q000201:enzymology* / Q000235:genetics* / Q000254:growth & development; D014764:Viral Proteins / Q000235:genetics
chemical_list: D004279:DNA, Viral; D014764:Viral Proteins; D000251:Adenosine Triphosphatases