Degradation of 2,4-dihydroxybenzoate by Pseudomonas sp. BN9.
The aerobic degradation of 2,4-dihydroxybenzoate by Pseudomonas sp. BN9 was studied. Intact cells of Pseudomonas sp. BN9 grown with 2,4-dihydroxybenzoate oxidized 2,4-dihydroxybenzoate but not salicylate. Cell-free extracts of Pseudomonas sp. BN9 converted 2,4-dihydroxybenzoate after the addition of NAD(P)H. A partially purified protein fraction converted 2,4-dihydroxybenzoate with NADH to 1,2,4-trihydroxybenzene. 1,2,4-Trihydroxybenzene was converted by a 1,2-dioxygenase to maleylpyruvate, which was reduced by a NADH-dependent enzyme to 3-oxoadipate. 2,4-Dihydroxybenzoate 1-monooxygenase, 1,2,4-trihydroxybenzene 1,2-dioxygenase and maleylpyruvate reductase were induced in Pseudomonas sp. BN9 after growth with 2,4-dihydroxybenzoate.
Stolz
A
A
Institut für Mikrobiologie, Universität Stuttgart, FRG.
Knackmuss
H J
HJ
D000332:Aerobiosis; D001673:Biodegradation, Environmental; D002474:Cell-Free System; D006873:Hydroquinones / Q000378:metabolism; D062385:Hydroxybenzoates / Q000378:metabolism*; D006899:Mixed Function Oxygenases / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D010084:Oxidation-Reduction; D010101:Oxygen Consumption; D011549:Pseudomonas / Q000378:metabolism*; D013053:Spectrophotometry
D006873:Hydroquinones; D062385:Hydroxybenzoates; C013038:hydroxyhydroquinone; D009249:NADP; D006899:Mixed Function Oxygenases; C017704:beta-resorcylic acid