B-->Z transition in native calf thymus DNA depends on its microenvironment.
Z-DNA forming potentiality of native calf thymus DNA was investigated under varying experimental conditions. Ethidium intercalation and Scatchard analysis data showed decreased binding of ethidium to nDNA at 4.27 M NaCl. A comparison of ethidium binding to DNA in low and high salt indicated the formation of Z-DNA. The binding of monoclonal anti-Z-DNA antibody to nDNA fragments brominated in high salt reiterated the transition of B-->Z conformation.
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Department of Biochemistry, Faculty of Medicine, A.M.U. Aligarh, India.
Ali
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D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism; D001966:Bromine / Q000378:metabolism; D002417:Cattle; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D004996:Ethidium / Q000378:metabolism; D007700:Kinetics; D009690:Nucleic Acid Conformation
D000911:Antibodies, Monoclonal; D004247:DNA; D004996:Ethidium; D001966:Bromine