Effects of inorganic phosphate analogues on stiffness and unloaded shortening of skinned muscle fibres from rabbit.
1. We examined the effects of aluminofluoride (AlFx) and orthovanadate (Vi), tightly binding analogues of orthophosphate (Pi), on the mechanical properties of glycerinated fibres from rabbit psoas muscle. Maximum Ca(2+)-activated force, stiffness, and unloaded shortening velocity (Vus) were measured under conditions of steady-state inhibition (up to 1 mM of inhibitor) and during the recovery from inhibition. 2. Stiffness was measured using either step or sinusoidal (1 kHz) changes in fibre length. Sarcomere length was monitored continuously by helium-neon laser diffraction during maximum Ca2+ activation. Stiffness was determined from the changes in sarcomere length and the corresponding changes in force. Vus was measured using the slack test method. 3. AlF chi and Vi each reversibly inhibited force, stiffness and Vus. Actively cycling cross-bridges were required for reversal of these inhibitory effects. Recovery from inhibition by AlF chi was 3- to 4-fold slower than that following removal of V1. 4. At various degrees of inhibition, AlF chi and Vi both inhibited steady-state isometric force more than either Vus or stiffness. For both AlF chi and Vi, the relatively greater inhibition of force over stiffness persisted during recovery from steady-state inhibition. We interpret these results to indicate that the cross-bridges with AlF chi or Vi bound are analogous to those which occur early in the cross-bridge cycle.
Chase
P B
PB
Department of Radiology, University of Washington, Seattle 98195.
Martyn
D A
DA
Kushmerick
M J
MJ
Gordon
A M
AM
D000535:Aluminum / Q000494:pharmacology; D017607:Aluminum Compounds; D000818:Animals; D002118:Calcium / Q000378:metabolism; D003864:Depression, Chemical; D005459:Fluorides / Q000494:pharmacology; D009119:Muscle Contraction / Q000187:drug effects*; D009132:Muscles / Q000187:drug effects*; D010710:Phosphates / Q000494:pharmacology*; D011817:Rabbits; D012518:Sarcomeres / Q000648:ultrastructure; D014638:Vanadates / Q000494:pharmacology
D017607:Aluminum Compounds; D010710:Phosphates; D014638:Vanadates; D000535:Aluminum; D005459:Fluorides; D002118:Calcium; C032311:aluminum fluoride