title: Early and long-term effects of colectomy and endorectal pullthrough on bile acid profile.
abstract: OBJECTIVE
  Although total colectomy with mucosal proctectomy and endorectal pullthrough affects two sites critical to the enterohepatic circulation of bile acids, little information is available regarding the manner in which normal digestive physiology is altered by these procedures. This study defines the early and long-term effects of colectomy and endorectal pullthrough on bile acid profile and the long-term effects on biliary lipid metabolism.
  
  
  BACKGROUND
  Specific changes in bile acid absorption have been reported in patients after ileal resection. Recent studies from our laboratory indicate that in the early postoperative period, colectomy with endorectal pullthrough causes a significant decrease in gallbladder bile concentrations of total bile acids, cholesterol, phospholipids, and calcium. The observation by several authors that the pouch undergoes morphologic and perhaps functional adaptation suggest that these changes may be transient and perhaps reversible.
  
  
  METHODS
  These studies were done in an awake, unanesthetized canine model that allows periodic sampling of gallbladder bile without creation of an external biliary fistula and its associated sequelae. Animals were ultimately randomly assigned to either laparotomy and gallbladder cannulation (N = 6), or gallbladder cannulation with total colectomy and ileorectal anastomosis (N = 7), or biliary cannulation, colectomy, mucosal proctectomy and endorectal pullthrough with ileal reservoir (N = 5).
  
  
  RESULTS
  Six weeks after operation, colectomy and ileorectal anastomosis were associated with a significant alteration in the relative composition of bile acids in gallbladder bile. These early changes were manifested by a significant (p < 0.05) increase in taurocholic acid and a concomitant decrease in taurodeoxycholic acid. These changes became even more pronounced in the ileorectal anastomosis group 12 weeks after colectomy and ileorectostomy. Although similar changes in the relative concentrations of individual bile acids occurred in the 6-week endorectal animals, bile acid profile was restored to normal by 12 weeks.
  
  
  CONCLUSIONS
  Colectomy with ileorectal anastomosis leads to early and significant changes in bile acid profile, which persist and become even more pronounced with time. In contrast, the construction of an ileal reservoir after colectomy facilitates restoration of a normal bile acid profile. We propose that these alterations in bile acid metabolism result from adaptation of the ileal reservoir as its mucosa assumes functional characteristics of normal colon.
authors: Research Service, Sepulveda Veterans Administration Medical Center, Los Angeles, California.
authors: R E
authors: RE
authors: Kelly
authors: M Z
authors: MZ
authors: Abedin
authors: E W
authors: EW
authors: Fonkalsrud
authors: J A
authors: JA
authors: Cates
authors: K
authors: K
authors: Saunders-Kirkwood
authors: H
authors: H
authors: Masuda
authors: E J
authors: EJ
authors: Huang
authors: J K
authors: JK
authors: Dhiman
authors: J J
authors: JJ
authors: Roslyn
mesh_terms: D000222:Adaptation, Physiological / Q000502:physiology; D000818:Animals; D001646:Bile / Q000737:chemistry / Q000378:metabolism*; D001647:Bile Acids and Salts / Q000378:metabolism*; D002784:Cholesterol / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D003082:Colectomy; D004285:Dogs; D005260:Female; D005704:Gallbladder / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000502:physiology; D010743:Phospholipids / Q000378:metabolism; D016737:Proctocolectomy, Restorative; D012007:Rectum / Q000601:surgery; D013997:Time Factors
chemical_list: D001647:Bile Acids and Salts; D010743:Phospholipids; D002784:Cholesterol