title: Oxidative modification of glucose-6-phosphate dehydrogenase from Leuconostoc mesenteroides by an iron(II)-citrate complex.
abstract: Incubation of glucose-6-phosphate dehydrogenase from Leuconostoc mesenteroides with Fe2+ and citrate results in rapid O2-dependent inactivation of the enzyme. Maximal rate of inactivation occurred at equimolar concentrations of Fe2+ and citrate. Loss of enzyme activity appears to be the result of selective oxidative modification, as evidenced by a corresponding increase in protein carbonyl content. Partially inactivated enzyme remained predominantly in the dimeric form with no change in the apparent affinity of the remaining active subunits for substrate. Modified Glu-6-PDH was, however, more susceptible to heat denaturation. Our results suggest that the Fe(2+)-citrate complex binds to the glucose 6-phosphate binding site and then undergoes reaction with H2O2 formed in solution leading to the oxidative modification of amino acids essential for enzyme activity.
authors: Laboratory of Biochemistry, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892.
authors: L I
authors: LI
authors: Szweda
authors: E R
authors: ER
authors: Stadtman
mesh_terms: D001665:Binding Sites; D002951:Citrates / Q000494:pharmacology; D019343:Citric Acid; D004795:Enzyme Stability; D005296:Ferrous Compounds / Q000494:pharmacology*; D005954:Glucosephosphate Dehydrogenase / Q000187:drug effects / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000378:metabolism; D007501:Iron / Q000494:pharmacology; D007934:Leuconostoc / Q000201:enzymology*; D010084:Oxidation-Reduction; D010100:Oxygen / Q000494:pharmacology
chemical_list: D002951:Citrates; D005296:Ferrous Compounds; D019343:Citric Acid; C016600:ferrous citrate; D006861:Hydrogen Peroxide; D007501:Iron; D005954:Glucosephosphate Dehydrogenase; D010100:Oxygen