Assay of artelinic acid in serum by high-performance liquid chromatography.
A simple and rapid high-performance liquid chromatographic method with UV detection for the determination in serum of the antimalarial artelinic acid is described. Artelinic acid is a water-soluble semi-synthetic derivative of artemisinin (or Qinghaosu), which is the parent compound of a completely new class of antimalarials with great potential value. Calibration curves of artelinic acid in serum from 0.5 to 50 micrograms/ml were found to be linear, and the extraction recovery was ca. 70% from rabbit serum and 45% from bovine and human serum. The detection limit of the method was 50 ng/ml when 250 microliters of serum were processed. Spiked samples (rabbit serum) proved to be stable for at least 43 days on storage at -20 degrees C. The method has been used for the analysis of ca. 400 samples in a pharmacokinetic study. About 50 samples can be processed in half a day.
Titulaer
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Department of Pharmaceutics, Faculty of Pharmacy, Utrecht University, Netherlands.
Vink-Blijleven
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N
D000818:Animals; D000962:Antimalarials / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D037621:Artemisinins; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007275:Injections, Intravenous; D011817:Rabbits; D012044:Regression Analysis; D012717:Sesquiterpenes / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics
D000962:Antimalarials; D037621:Artemisinins; D012717:Sesquiterpenes; C062768:artelinic acid