Halothane anesthesia is neuroprotective in experimental spinal cord injury: early hemodynamic mechanisms of action.
The neuroprotective potential of halothane anesthesia was explored in a weight-drop model of spinal trauma in the rat (N = 252). In initial experiments, animals were subjected to 25, 50 or 100 g cm impact injuries at T10 during pentobarbital or halothane anesthesia and their outcomes determined using somatosensory-evoked potentials, blinded neurologic evaluations for two weeks, and post-mortem analysis of spinal serotonin levels. Subsequently, halothane anesthesia was combined with either pentobarbital or nitrous oxide or given as a late treatment to pentobarbital anesthetized rats subjected to 50 g cm injuries. A series of acute studies were then performed in order to assess the hemodynamic and respiratory concomitants of halothane vs. pentobarbital, as well as the effect of mechanical ventilation and bicarbonate treatment upon halothane neuroprotection. Finally, the effect of a 50 g cm impact upon local white matter spinal cord blood flow was measured during halothane or pentobarbital anesthesia using laser-Doppler flowmetry. Results demonstrate an active neuroprotective action for halothane anesthesia that is not altered by the presence of other anesthetics and is most prominent at severe injury levels. The data suggest the importance of immediate injury responses in this action. Late halothane treatment was ineffective when given as early as 10 minutes postinjury while both the electrophysiological and hemodynamic effects of halothane vs. pentobarbital were apparent during this 10 minute period. Thus, halothane was associated with the prevention of spinal ischemia during the first 10 minutes after trauma in comparison to pentobarbital.(ABSTRACT TRUNCATED AT 250 WORDS)
Salzman
S K
SK
Alfred I. duPont Institute, Spinal Trauma Research Program, Wilmington, Delaware 19899.
Lee
W A
WA
Sabato
S
S
Mendez
A A
AA
Agresta
C A
CA
Kelly
G
G
D000769:Anesthesia, Inhalation; D000818:Animals; D004347:Drug Interactions; D005073:Evoked Potentials, Somatosensory / Q000187:drug effects; D006221:Halothane / Q000494:pharmacology*; D006439:Hemodynamics / Q000187:drug effects*; D008297:Male; D009043:Motor Activity / Q000187:drug effects; D009609:Nitrous Oxide / Q000494:pharmacology; D010424:Pentobarbital / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000098:blood supply; D013119:Spinal Cord Injuries / Q000503:physiopathology* / Q000517:prevention & control
D010424:Pentobarbital; D009609:Nitrous Oxide; D006221:Halothane