Characterization of the response of erythrocyte sodium-lithium countertransport to inhibitors.
To clarify the biochemical characteristics of the sodium-lithium countertransporter (SLC) the responses of SLC to two probes of ion transport--4,4'-diisothiocyanostilbene-2,2' disulfonate, and N-ethylmaleimide--were assessed. The results suggest that the SLC transporter is associated with the band 3 protein in the plasma membrane and demonstrate that a sulfhydryl group is essential for transport function.
Rutherford
P A
PA
Department of Medicine (Nephrology) Freeman Hospital, University of Newcastle-upon-Tyne, United Kingdom.
Thomas
T H
TH
Wilkinson
R
R
D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D017920:Antiporters; D001692:Biological Transport / Q000187:drug effects; D002352:Carrier Proteins / Q000097:blood*; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism*; D005033:Ethylmaleimide / Q000494:pharmacology*; D005260:Female; D006801:Humans; D008094:Lithium / Q000097:blood*; D008297:Male; D012964:Sodium / Q000097:blood*
D017920:Antiporters; D002352:Carrier Proteins; C045885:sodium-lithium countertransporter; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid; D008094:Lithium; D012964:Sodium; D005033:Ethylmaleimide; D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid