Replication timing and Xenopus 5S RNA gene transcription in vitro.
The influence of DNA replication in a chromosomal environment on the transcription of Xenopus laevis 5S RNA genes has been examined using low-speed extracts of Xenopus eggs and Xenopus sperm nuclei. The major oocyte- and somatic-type 5S RNA multigene families of X. laevis are differentially expressed in these extracts dependent on limiting transcription factors and replicate in different compartments of S-phase. Under these particular experimental conditions the timing of replication of the somatic 5S RNA genes appears to be independent of their transcriptional activity. Moreover, differential transcription of the oocyte- and somatic-type 5S RNA genes is not influenced by the replication process.
Wolffe
A P
AP
Laboratory of Molecular Embryology, National Institute of Child Health and Human Development NIH, Bethesda, Maryland 20892.
D000818:Animals; D002467:Cell Nucleus / Q000502:physiology; D004261:DNA Replication; D004275:DNA, Ribosomal / Q000235:genetics*; D005260:Female; D007700:Kinetics; D008297:Male; D005810:Multigene Family; D009865:Oocytes / Q000502:physiology*; D012341:RNA, Ribosomal, 5S / Q000235:genetics*; D013084:Sperm-Ovum Interactions; D013094:Spermatozoa / Q000502:physiology*; D013997:Time Factors; D035582:Transcription Factor TFIIIA; D014157:Transcription Factors / Q000378:metabolism; D014158:Transcription, Genetic; D014982:Xenopus laevis / Q000235:genetics*
D004275:DNA, Ribosomal; D012341:RNA, Ribosomal, 5S; D035582:Transcription Factor TFIIIA; D014157:Transcription Factors