title: Liquid chromatographic analysis of niacin in fortified food products.
abstract: An ion exchange liquid chromatographic (LC) method using an anion exchange resin column was developed for the determination of niacin in fortified foods. Samples were extracted by autoclaving with H2SO4 (1 + 1). Florisil open column chromatography was used to remove interferences from the sample extracts. Niacin levels were quantitated by an LC system using a 250 x 4.1 mm Hamilton PRP-X100 column, a mobile phase of 2% glacial acetic acid in water, and UV detection at 254 nm. The limit of detection was 0.11 micrograms niacin/mL, and the standard curve was linear from 0.24 to 0.80 micrograms niacin/mL. The system reproducibility was evaluated by completing 10 repetitive analyses on an infant formula and a macaroni product, which gave an average CV of 2.7%. Mean recovery (+/- standard deviation) was 99.8 +/- 7.7 (n = 15). The results compared favorably with those by the AOAC microbiological method.
authors: U.S. Food and Drug Administration, Atlanta, GA 30309.
authors: G W
authors: GW
authors: Chase
authors: W O
authors: WO
authors: Landen
authors: A G
authors: AG
authors: Soliman
authors: R R
authors: RR
authors: Eitenmiller
mesh_terms: D002852:Chromatography, Ion Exchange / Q000379:methods / Q000592:standards; D005527:Food, Fortified / Q000032:analysis*; D017633:Magnesium Silicates; D009525:Niacin / Q000032:analysis* / Q000592:standards; D012015:Reference Standards; D012824:Silicic Acid
chemical_list: D017633:Magnesium Silicates; C005013:Florisil; D012824:Silicic Acid; D009525:Niacin