Preliminary X-ray study of naproxen esterase from Bacillus subtilis.
Single crystals of naproxen esterase from Bacillus subtilis have been obtained from PEG6000 solutions at pH 8.0 by liquid-liquid diffusion while applying a temperature gradient from 4 degrees C to room temperature over a period of four weeks. The crystals belong to the trigonal space group P3(1)21 or P3(2)21 with a = b = 47.59 A and c = 212.91 A. The asymmetric part of the unit cell contains one protein molecule with M(r) = 33,771. The crystals diffract to at least 3.0 A resolution and are suitable for an X-ray structure analysis.
van der Laan
J M
JM
Royal Gist-brocades N.V. Research and Development, Delft, The Netherlands.
Teplyakov
A V
AV
Lammers
A A
AA
Dijkstra
B W
BW
D001412:Bacillus subtilis / Q000201:enzymology*; D043182:Carboxylesterase; D002265:Carboxylic Ester Hydrolases / Q000737:chemistry* / Q000302:isolation & purification; D003460:Crystallization; D011487:Protein Conformation; D014961:X-Ray Diffraction / Q000379:methods
D002265:Carboxylic Ester Hydrolases; D043182:Carboxylesterase