Mitogenicity of the earthworm's (Eisenia foetida) insulin-like proteins.
1. Biologically active glycolipoprotein complex (G-90), isolated from whole earthworm tissue extract (Eisenia foetida), was separated into seven fractions by gel-filtration. 2. It has been shown by radioimmunoassay that each of the fractions, except the lightest one, is cross-reactive with porcine anti-insulin antibodies. Molecules that possess such activity were detected by immunoblotting. 3. All fractions, except the heaviest and the lightest one, stimulate mammalian normal and transformed cell proliferation in serum-free conditions in vitro. The intensity of stimulation depends on cell type. Stimulation is completely abolished if the medium is supplemented with fetal calf serum.
Hrzenjak
M
M
Department of Molecular Oncology, Ruder Boscović Institute, Zagreb, Croatia.
Kobrehel
D
D
Levanat
S
S
Jurin
M
M
Hrzenjak
T
T
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002850:Chromatography, Gel; D006224:Cricetinae; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005354:Fibrosarcoma; D006023:Glycoproteins / Q000302:isolation & purification; D006801:Humans; D015151:Immunoblotting; D007328:Insulin / Q000302:isolation & purification / Q000494:pharmacology*; D008074:Lipoproteins / Q000302:isolation & purification; D008297:Male; D008546:Melanoma, Experimental; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008934:Mitogens; D009835:Oligochaeta; D011863:Radioimmunoassay; D014407:Tumor Cells, Cultured; D002583:Uterine Cervical Neoplasms
D006023:Glycoproteins; D007328:Insulin; D008074:Lipoproteins; D008934:Mitogens