title: Bound lipids liberated by alkaline hydrolysis after exhaustive extraction of pulverized clavus.
abstract: In the present study, covalently bound lipids were found in clavus material and their lipid classes were determined by high-performance thin-layer chromatography (HPTLC). Clavus material, pulverized completely in a Mikro-Dismembrator II, was exhaustively extracted three times with chloroform/methanol (2:1, 1:1 and 1:2 v/v) at 80 degrees C for 1 h each time and this sequence of extractions was repeated to obtain the unbound lipid-free residue which was saponified and then extracted with chloroform. The extract proved to comprise several bands of lipids covalently bound through an ester-like linkage. These were identified as free fatty acids, cholesterol, ceramides and glucocerebrosides by HPTLC. However, omega-hydroxy fatty acids were not detected in the lipids. To analyse the fatty acids amide-linked to the bound ceramides, the latter were isolated by preparative HPTLC and subjected to mild acid hydrolysis. Since the bound ceramides constituted neither omega-hydroxy fatty acids nor alpha-hydroxy fatty acids, they were not identified as hydroxyl-acylsphingosines.
authors: Department of Dermatology, Niigata University School of Medicine, Japan.
authors: S
authors: S
authors: Serizawa
authors: M
authors: M
authors: Ito
authors: S
authors: S
authors: Hamanaka
authors: F
authors: F
authors: Otsuka
mesh_terms: D002855:Chromatography, Thin Layer; D004817:Epidermis / Q000737:chemistry*; D005227:Fatty Acids / Q000032:analysis; D006801:Humans; D006868:Hydrolysis; D008055:Lipids / Q000032:analysis*
chemical_list: D005227:Fatty Acids; D008055:Lipids