The primary structure of rabbit serum amyloid A protein isolated from acute phase serum.
Serum amyloid A (SAA) protein, a sensitive acute phase protein and the precursor of protein AA in secondary amyloid, was purified from pooled acute phase rabbit serum using two different methods: isolation of protein SAA directly by octyl-Sepharose chromatography of total serum, and dissociation and isolation of apoSAA from acute phase high density lipoprotein (HDL). The protein SAA fraction obtained was further purified using gel filtration and ion exchange chromatography. Rabbit protein SAA has 104 amino acid residues, like human SAA, and has a partially blocked N terminus. The highly conserved region from position 33 to position 63 found in SAA from all species studied was confirmed also in rabbit SAA. No microheterogeneities were observed. The amino acid sequence showed extensive N-terminal homology with the rabbit amyloid A protein, except for the microheterogeneity in position 12 in protein AA. It also showed identical amino acid sequence with that deduced from the rabbit cDNA clone pSAA 55. Complete homologies were found with clone SAA 2, except for positions 22 and 78, clone SA8-1, except for positions 22 and 79 and clone SA7-3, except for position 22. This pSAA 55/SA7-3/SA8-1/SAA2-like protein was the only SAA isotype found both in total serum and in the HDL fraction. Isotypes corresponding to other SAA-like genes could not be found in this pool of acute phase rabbit sera.
Syversen
P V
PV
Department of Biochemistry/Biotechnology Centre of Oslo, Oslo University, Norway.
Juul
J
J
Rygg
M
M
Sletten
K
K
Husby
G
G
Marhaug
G
G
D000210:Acute-Phase Reaction / Q000097:blood*; D000595:Amino Acid Sequence; D000818:Animals; D017124:Conserved Sequence; D003488:Cyanogen Bromide; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry; D011817:Rabbits; D017386:Sequence Homology, Amino Acid; D000685:Serum Amyloid A Protein / Q000737:chemistry* / Q000302:isolation & purification
D010455:Peptides; D000685:Serum Amyloid A Protein; D003488:Cyanogen Bromide