title: Crystallization and preliminary crystallographic analysis of a tetrameric isolectin from Vicia villosa, specific for the Tn antigen.
abstract: Isolectin B4 isolated from Vicia villosa seeds is specific for the Tn antigen, a carcinoma-associated molecular marker. Crystals of the isolectin grown in the presence of carbohydrate are tetragonal, space group P4(1) (or P4(3), with a = 91.3 A, c = 151.7 A and one tetramer in the asymmetric unit. The crystals diffract X-rays to 2.8 A resolution and are suitable for high-resolution structural analysis.
authors: Unité de Neurobiologie Moléculaire URA 1284 CNRS, Institut Pasteur, Paris, France.
authors: J L
authors: JL
authors: Eiselé
authors: D
authors: D
authors: Tello
authors: E
authors: E
authors: Osinaga
authors: A
authors: A
authors: Roseto
authors: P M
authors: PM
authors: Alzari
mesh_terms: D000116:Acetylgalactosamine; D015295:Antigens, Tumor-Associated, Carbohydrate; D003460:Crystallization; D006801:Humans; D037102:Lectins / Q000737:chemistry* / Q000302:isolation & purification; D046911:Macromolecular Substances; D014961:X-Ray Diffraction / Q000379:methods
chemical_list: D015295:Antigens, Tumor-Associated, Carbohydrate; D037102:Lectins; D046911:Macromolecular Substances; C040286:Tn antigen; D000116:Acetylgalactosamine