title: A bromodeoxyuridine immunocytochemical and morphometric study of the influence of growth hormone on cell proliferation in odontogenic mesenchyme of the Lewis dwarf rat.
abstract: Cell proliferation was studied in pre-odontoblasts, and in cells of the dental papilla and lingual dental follicle using bromodeoxyuridine immunocytochemistry and morphometry in Bouin's perfused and paraffin-embedded, undemineralized maxillary incisors. Cells in DNA synthesis, as shown by this technique, or in mitosis, were counted. Significantly fewer labelled nuclei, unlabelled nuclei and total nuclei were found in the tissues of growth hormone-deficient dwarf rats than in normal tissues. However, in dwarf rats treated for 6 days with bovine growth hormone, their numbers were equivalent to, or in some instances greater than those in normal tissues. The bromodeoxyuridine labelling index, the ratio of positive to negative nuclei and the mitotic index of pre-odontoblasts in dwarf rats were consistently lower than in normal rats, and were reversible by growth hormone. Growth hormone thus plays a part in odontogenic mesenchymal proliferation.
authors: Division of Oral Biology and Pathology, Faculty of Dentistry, University of Queensland, Australia.
authors: W G
authors: WG
authors: Young
authors: C Z
authors: CZ
authors: Zhang
authors: H
authors: H
authors: Li
authors: P E
authors: PE
authors: Lobie
authors: M J
authors: MJ
authors: Waters
mesh_terms: D000565:Ameloblasts / Q000187:drug effects / Q000378:metabolism; D000704:Analysis of Variance; D000818:Animals; D001973:Bromodeoxyuridine / Q000378:metabolism; D002455:Cell Division / Q000187:drug effects*; D004247:DNA / Q000096:biosynthesis; D003771:Dental Papilla / Q000187:drug effects / Q000378:metabolism; D003795:Dental Sac / Q000187:drug effects / Q000378:metabolism; D013006:Growth Hormone / Q000494:pharmacology / Q000502:physiology*; D007150:Immunohistochemistry; D008297:Male; D008648:Mesoderm / Q000502:physiology; D008938:Mitosis / Q000187:drug effects; D008940:Mitotic Index; D009804:Odontoblasts / Q000187:drug effects / Q000378:metabolism*; D009805:Odontogenesis; D051381:Rats; D011917:Rats, Inbred Lew; D016196:S Phase / Q000187:drug effects
chemical_list: D013006:Growth Hormone; D004247:DNA; D001973:Bromodeoxyuridine