title: Myocardial antioxidant defenses during cardiopulmonary bypass.
abstract: In 31 male patients undergoing coronary bypass surgery who underwent different periods of cardioplegic hypothermic arrest, the activities of glutathione peroxidase, glutathione reductase, glutathione transferase, copper/zinc-containing and manganese-containing superoxide dismutases, and catalase were studied in the right atrial myocardium, before and 5 minutes after aortic cross-clamping. The levels of thiobarbituric acid reactive substances (TBARS) and nonproteic thiol compounds (NP-SH) were also assessed. Prolonged ischemia followed by reperfusion induced activation of the major myocardial antioxidant enzymes with marked NP-SH depression and TBARS increase, despite cold crystalloid cardioplegic protection. These changes were significantly related to the duration of the ischemic arrest, suggesting: (1) that reperfusion free radical generation is dependent on the severity of the previous ischemic period; and (2) the occurrence of myocardial oxidative stress during cardiopulmonary bypass.
authors: Laboratorio di Fisiopatologia Medica Sperimentale, Cattedra di Patologia Medica, Università di Chieti, Italy.
authors: A
authors: A
authors: Mezzetti
authors: D
authors: D
authors: Lapenna
authors: S D
authors: SD
authors: Pierdomenico
authors: G
authors: G
authors: Di Giammarco
authors: G
authors: G
authors: Bosco
authors: C
authors: C
authors: Di Ilio
authors: P
authors: P
authors: Santarelli
authors: A M
authors: AM
authors: Calafiore
authors: F
authors: F
authors: Cuccurullo
mesh_terms: D000328:Adult; D000975:Antioxidants / Q000378:metabolism; D002315:Cardiopulmonary Bypass; D002374:Catalase / Q000378:metabolism; D003327:Coronary Disease / Q000201:enzymology*; D005979:Glutathione Peroxidase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D006324:Heart Arrest, Induced; D006325:Heart Atria / Q000201:enzymology; D006801:Humans; D008297:Male; D008875:Middle Aged; D009206:Myocardium / Q000201:enzymology*; D013438:Sulfhydryl Compounds / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D013997:Time Factors
chemical_list: D000975:Antioxidants; D013438:Sulfhydryl Compounds; D017392:Thiobarbituric Acid Reactive Substances; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase; D005982:Glutathione Transferase