A multi-centre evaluation of the measurement of high density lipoprotein cholesterol by the Reflotron assay.
A multi-centre study was undertaken to evaluate the performance of the Reflotron high density lipoprotein cholesterol method in twelve different clinical chemistry hospital laboratories. Results were compared with phosphotungistic acid/Mg2+ and polyethylene glycol/dextran sulphate/Mg2+ precipitation methods, routinely performed in these laboratories. Precision was found to be excellent, for both the day-to-day results (SD = 0.03 mmol/l) and for the overall between-laboratory precision (SD = 0.02 mmol/l), measured with control sera. HDL cholesterol concentrations measured with the Reflotron system were about 10% lower than those obtained with the two routine precipitation methods, using different instruments with different cholesterol standardization procedures.
Bijster
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Clinical Laboratory, Martini Ziekenhuis, Groningen, The Netherlands.
D002624:Chemistry, Clinical / Q000379:methods*; D008076:Cholesterol, HDL / Q000097:blood*; D006801:Humans; D008274:Magnesium / Q000737:chemistry; D010772:Phosphotungstic Acid; D011092:Polyethylene Glycols; D015203:Reproducibility of Results
D008076:Cholesterol, HDL; D010772:Phosphotungstic Acid; D011092:Polyethylene Glycols; D008274:Magnesium