Actions of a monoclonal antibody Tor 23 on rat brain presynaptic cholinergic processes.
Tor 23 is a monoclonal antibody, generated against cholinergic terminals of the Torpedo californica, that has been found to bind to the extracellular surface of cholinergic neurons in a variety of tissues. This study shows that Tor 23 inhibits: 1) high affinity [3H]hemicholinium-3 binding to detergent-solubilized membranes prepared from rat neocortices; 2) high affinity [3H]choline uptake in rat neocortical and striatal P2 preparations; and 3) [3H]acetylcholine synthesis in isolated nerve terminals. Tor 23 does not appear to affect low affinity [3H]choline uptake or [3H]acetylcholine release. These results are consistent with the hypothesis that Tor 23 may bind to nerve terminal high affinity choline transporters in the rat brain.
Evans
S M
SM
Dept. of Pharmacology and Therapeutics, University of Florida College of Medicine, Gainesville 32610-0267.
Kushner
P D
PD
Meyer
E M
EM
D000109:Acetylcholine / Q000096:biosynthesis; D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism*; D001921:Brain / Q000276:immunology / Q000378:metabolism*; D002352:Carrier Proteins / Q000276:immunology; D002462:Cell Membrane / Q000378:metabolism; D002540:Cerebral Cortex / Q000378:metabolism; D002794:Choline / Q000378:metabolism*; D003342:Corpus Striatum / Q000378:metabolism; D006426:Hemicholinium 3 / Q000378:metabolism; D008297:Male; D009411:Nerve Endings / Q000276:immunology / Q000378:metabolism; D009474:Neurons / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
D000911:Antibodies, Monoclonal; D002352:Carrier Proteins; D006426:Hemicholinium 3; D002794:Choline; D000109:Acetylcholine