title: Sulfite inhibits oxalate production from glycolate and glyoxylate in vitro and from dichloroacetate infused i.v. into male rats.
abstract: The effect of sulfite on oxalate production from glycolate and glyoxylate was investigated in an in vitro assay system using rabbit muscle LDH and rat liver 35-60% ammonium sulfate fraction as enzymes. A > or = 50% inhibition in oxalate production was observed at sulfite to glyoxylate ratio of 0.4. LDH activity (change in A340 nm) was inhibited by > or = 40% at sulfite to glyoxylate ratio of 0.5. Male Sprague-Dawley rats fasted for 24 h and infused with dichloroacetate (150 mg/rat/h, iv, n = 6), without and with sodium sulfite (150 mg/rat/h, n = 6) excreted oxalate in urine, respectively, at the rate of 1.13 +/- 0.29 and 0.61 +/- 0.09 mumol/h/100 g body wt, showing a nearly 50% reduction due to sulfite. The present report demands further experimentation to define the role of sulfite as an intracellular modulator of oxalate production.
authors: Division of Experimental Surgery, University of Erlangen, Germany.
authors: V
authors: V
authors: Sharma
authors: P O
authors: PO
authors: Schwille
mesh_terms: D000429:Alcohol Oxidoreductases / Q000378:metabolism; D000818:Animals; D003999:Dichloroacetic Acid / Q000494:pharmacology*; D006016:Glycolates / Q000378:metabolism*; D006038:Glyoxylates / Q000378:metabolism*; D007770:L-Lactate Dehydrogenase / Q000037:antagonists & inhibitors / Q000378:metabolism; D008099:Liver / Q000201:enzymology; D008297:Male; D009132:Muscles / Q000201:enzymology; D010070:Oxalates / Q000378:metabolism*; D019815:Oxalic Acid; D011817:Rabbits; D051381:Rats; D017207:Rats, Sprague-Dawley; D013447:Sulfites / Q000494:pharmacology*
chemical_list: D006016:Glycolates; D006038:Glyoxylates; D010070:Oxalates; D013447:Sulfites; C031149:glycolic acid; D019815:Oxalic Acid; D003999:Dichloroacetic Acid; D000429:Alcohol Oxidoreductases; D007770:L-Lactate Dehydrogenase; C020821:glycollate oxidase; C031150:glyoxylic acid; C025026:sodium sulfite