Functional recovery in rabbit heart after preservation with a blood cardioplegic solution and perfusion.
Thirty-five isolated rabbit hearts were subjected to 60 minutes of storage after cardiac arrest with high K+ crystalloid cardioplegic solution at 5 degrees C. They were divided into five groups (n = 7 per group) according to the preservative protocol: group I (simple immersion in 0 degrees C normal saline solution), group II (simple immersion in 30 degrees C blood cardioplegic solution), group III (immersion and perfusion in 30 degrees C blood cardioplegic solution), group IV (simple immersion in 10 degrees C blood cardioplegic solution), and group V (immersion and perfusion in 10 degrees C blood cardioplegic solution). After storage for 1 hour, cardiac function, tissue water content, and the number of hearts capable of ejecting against a 100 cm H2O column afterload were recorded and compared among the five groups. Cardiac function in group II was not different from group I. Aortic pulse pressure and left ventricular developed pressure were lower in group II than in group III, IV, or V. Aortic developed pressure was higher in groups III and V (p < 0.05). Tissue water content in group I was significantly higher than in groups II, III, and V (p < 0.05). All hearts in groups III and V could eject against a 100 cm H2O afterload after preservation (p < 0.05 versus groups I, II, and IV). Factorial analysis among the groups using blood cardioplegic solution showed that either hypothermia or perfusion alone or the combination was a protective factor in providing functional recovery of stored hearts.(ABSTRACT TRUNCATED AT 250 WORDS)
Li
G
G
Department of Cardiovascular Surgery, Dalhousie University, Victoria General Hospital, Halifax, Nova Scotia, Canada.
Sullivan
J A
JA
Hall
R I
RI
D000818:Animals; D006321:Heart / Q000503:physiopathology*; D016027:Heart Transplantation; D006439:Hemodynamics; D009926:Organ Preservation / Q000379:methods; D010477:Perfusion; D011188:Potassium; D017680:Potassium Compounds; D011817:Rabbits
D017680:Potassium Compounds; C018369:potassium cardioplegic solution; D011188:Potassium