title: Changing patterns of the IL-2/IL-2R pathway of lymphocyte activation following exposure to Plasmodium falciparum products: a study with squirrel monkey peripheral blood mononuclear cells.
abstract: Squirrel monkeys are experimental hosts useful for studies on human malaria. In the present work, in vitro lymphocyte reactivity was measured by proliferation in the presence of Plasmodium falciparum-derived products, and found to depend on the previous malarial status of the animals and upon the source and/or the nature of the P. falciparum-derived material. Special attention was given to the IL-2/IL-2R pathway of lymphocyte activation. Culture supernatant from P. falciparum-parasitized erythrocytes exerted an inhibitory effect towards T lymphocytes obtained from P. falciparum-non-immune squirrel monkeys, when activated, for instance, by PHA. These lymphocytes did not incorporate tritiated thymidine (neither they did proliferate) although they expressed IL-2 alpha and beta binding chains and secreted IL-2 (or at least TCGF). This inhibitory effect could not be rescued by the addition of rhIL-2, although the assayed lymphocytes could retain the ability to continue their cell cycle progression and divide after removal of the P. falciparum-derived inhibitory product(s). The incidence of anti-mitogenic molecules which impair the IL-2/IL-2R pathway of lymphocyte activation in malaria related processes is discussed.
authors: Laboratoire d'Immunologie Parasitaire, Institut Pasteur de Guyane, Cayenne, French Guiana.
authors: O
authors: O
authors: Garraud
mesh_terms: D000818:Animals; D000953:Antigens, Protozoan / Q000276:immunology*; D002478:Cells, Cultured; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D005260:Female; D005434:Flow Cytometry; D007375:Interleukin-1 / Q000494:pharmacology; D007376:Interleukin-2 / Q000096:biosynthesis* / Q000276:immunology; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D008213:Lymphocyte Activation; D016778:Malaria, Falciparum / Q000276:immunology; D008297:Male; D010835:Phytohemagglutinins; D010963:Plasmodium falciparum / Q000276:immunology*; D015375:Receptors, Interleukin-2 / Q000096:biosynthesis*; D012453:Saimiri
chemical_list: D000953:Antigens, Protozoan; D007375:Interleukin-1; D007376:Interleukin-2; D010835:Phytohemagglutinins; D015375:Receptors, Interleukin-2