title: A 19F NMR study of the interaction of 3-fluoro-4-demethoxydaunomycin with the hexanucleotide d(TCCGGA)2.
abstract: 19F NMR spectroscopy has been used to study the binding of the fluorinated anthracycline 3-fluoro-4-demethoxydaunomycin with the hexanucleotide d(TCCGGA)2. In the spectrum of the 1:1 anthracycline-d(TCCGGA)2 complex four resonances of approximately equal intensity were observed. This indicated that 4-demethoxydaunomycin intercalated at all possible sites with similar affinity. This suggests that the specific high affinity binding sites that are observed in anthracycline-DNA footprinting experiments are strongly regulated by the local DNA conformation.
authors: Department of Chemistry, University College, University of New South Wales, Australia.
authors: K E
authors: KE
authors: Barr
authors: R A
authors: RA
authors: Russell
authors: R N
authors: RN
authors: Warrener
authors: J G
authors: JG
authors: Collins
mesh_terms: D001483:Base Sequence; D003630:Daunorubicin / Q000031:analogs & derivatives* / Q000378:metabolism; D005461:Fluorine; D007364:Intercalating Agents / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D009838:Oligodeoxyribonucleotides / Q000378:metabolism*
chemical_list: D007364:Intercalating Agents; D009838:Oligodeoxyribonucleotides; C065327:3-fluoro-4-demethoxydaunomycin; D005461:Fluorine; D003630:Daunorubicin