Mechanisms of cartilage degradation in inflammatory arthritis: interaction between chondrocytes and immunoglobulin G.
A possible role for immune complexes in the degradation of cartilage (rheumatoid arthritis and antigen induced arthritis) has been modelled in vitro by studying interactions between cultured bovine chondrocytes and monomeric (M) or heat aggregated (HA) IgG. Concentrations of IgG used were within the range of values reported in the synovial fluids of rheumatoid joints. ELISA and rosetting assays revealed Fc receptor mediated binding of MIgG and HAIgG to chondrocytes that had been cultured, but not to freshly isolated cells. Both forms of IgG stimulated the production of metalloprotease, but only HAIgG boosted generation of superoxide anion and reduced proteoglycan synthesis. HAIgG also stimulated cells to produce immunoreactive interleukin 1 although no biological activity was apparent. It is concluded that the equivalent behavior of chondrocytes in vivo, triggered by immune complexes, could contribute or lead directly to matrix degradation.
Saura
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R
Department of Surgery, Apps Medical Research Center, Queen's University, Kingston, Ontario, Canada.
Uno
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K
Satsuma
S
S
Kurz
E U
EU
Scudamore
R A
RA
Cooke
T D
TD
D000818:Animals; D001168:Arthritis / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D001666:Binding Sites, Antibody; D002358:Cartilage, Articular / Q000276:immunology* / Q000378:metabolism / Q000473:pathology*; D002417:Cattle; D002450:Cell Communication / Q000276:immunology*; D002478:Cells, Cultured; D006358:Hot Temperature; D007074:Immunoglobulin G / Q000737:chemistry / Q000145:classification / Q000378:metabolism*; D007375:Interleukin-1 / Q000032:analysis; D046911:Macromolecular Substances; D012397:Rosette Formation; D013329:Structure-Activity Relationship
D007074:Immunoglobulin G; D007375:Interleukin-1; D046911:Macromolecular Substances