Separation of beta-carotene and lycopene geometrical isomers in biological samples.
The analysis of beta-carotene and lycopene, the two predominant carotenoids in human serum and tissues, was extended to the level of geometrical (cis-trans) isomers by using an improved reversed-phase HPLC methodology. We separated five geometrical isomers of beta-carotene and seven of lycopene in human serum and tissues. 13-cis-beta-Carotene was identified as the predominant cisisomer in human serum, contributing about 5% to total beta-carotene. In tissue, however, considerable amounts of 9-cis- and traces of 15-cis-beta-carotene were also detected. In contrast to beta-carotene, the lycopene isomer patterns in human serum and tissues are quite similar.
Stahl
W
W
Institut für Physiologische Chemie I, Universität Düsseldorf, Germany.
Sundquist
A R
AR
Hanusch
M
M
Schwarz
W
W
Sies
H
H
D000311:Adrenal Glands / Q000737:chemistry; D000328:Adult; D002338:Carotenoids / Q000097:blood / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005260:Female; D006801:Humans; D008099:Liver / Q000737:chemistry; D000077276:Lycopene; D008297:Male; D008875:Middle Aged; D013237:Stereoisomerism; D019207:beta Carotene
D019207:beta Carotene; D002338:Carotenoids; D000077276:Lycopene