Organ specificity of the dopamine1 receptor/adenylyl cyclase coupling defect in spontaneously hypertensive rats.
The coupling between the dopamine1 (DA1) receptor and the G protein/adenylyl cyclase (AC) enzyme complex is defective in the proximal convoluted tubule (PCT) of 20-wk-old spontaneously hypertensive rats (SHRs). Because this coupling defect could have been due to desensitization secondary to elevated renal dopamine levels in the adult animal, we studied the interaction between DA1 receptors and AC in PCT of rats as early as 3 wk of age, a time when renal dopamine levels are similar in SHRs and their normotensive controls (Wistar-Kyoto rats, WKYs). Maximum receptor density did not change with age and was similar in WKYs and SHRs in all the age groups studied (3, 8, and 20 wk). Basal-, forskolin-, and guanyl nucleotide-stimulated AC activities were also similar in WKYs and SHRs and did not change with age. However, the DA1 agonist-stimulated AC activity was greater in WKYs than in SHRs and increased with age in WKYs but not in SHRs. Moreover, the ability of a nonhydrolyzable analogue of GTP, Gpp(NH)p, to enhance DA1 agonist (SND-919-C12, 1 microM)-stimulated AC activity increased with age in WKY but not in SHRs. To determine if the defect noted in the PCT of SHRs is due to a defective D1A receptor gene, parallel studies were performed in the striatum, since this receptor is expressed predominantly in the latter tissue. In contrast to the results in PCT, radioligand binding and AC studies in striatum revealed no differences between WKYs and SHRs.(ABSTRACT TRUNCATED AT 250 WORDS)
Felder
R A
RA
Department of Pathology, University of Virginia, Charlottesville 22908.
Kinoshita
S
S
Ohbu
K
K
Mouradian
M M
MM
Sibley
D R
DR
Monsma
F J
FJ
Minowa
T
T
Minowa
M T
MT
Canessa
L M
LM
Jose
P A
PA
D000262:Adenylyl Cyclases / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D001345:Autoradiography; D005576:Colforsin / Q000494:pharmacology; D003342:Corpus Striatum / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D006973:Hypertension / Q000235:genetics / Q000378:metabolism*; D007687:Kidney Tubules, Proximal / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D008297:Male; D009928:Organ Specificity; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D051381:Rats; D011918:Rats, Inbred SHR / Q000235:genetics*; D011921:Rats, Inbred WKY / Q000235:genetics*; D011954:Receptors, Dopamine / Q000032:analysis / Q000235:genetics / Q000378:metabolism*
D012333:RNA, Messenger; D011954:Receptors, Dopamine; D005576:Colforsin; D000262:Adenylyl Cyclases