Second branchial arch anomalies induced by fluconazole, a bis-triazole antifungal agent, in cultured mouse embryos.
The teratogenic potential of the novel bis-triazole antifungal agent fluconazole (F) was investigated on mouse embryos during early organogenesis using the whole-embryo culture system. Early somite stage embryos (4-5 somites) were continuously cultured for 48 hours in rat serum added with 0, 25, 50 or 75 micrograms/ml of F. Doses of 50 and 75 micrograms/ml of F significantly (p < 0.001) impaired morphogenesis, resulting in a 83.3% and 91.6% of embryos showing second branchial arch anomalies, respectively. These findings demonstrate that F induces second branchial arch anomalies in cultured mouse conceptuses. Furthermore, these results may suggest an useful model for the study of arches-derived malformations.
Tiboni
G M
GM
Clinica Ostetrica e Ginecologica, Università G.D'Annunzio, Chieti, Italy.
D000014:Abnormalities, Drug-Induced / Q000209:etiology*; D000818:Animals; D001934:Branchial Region / Q000002:abnormalities*; D046508:Culture Techniques; D004622:Embryo, Mammalian / Q000187:drug effects; D005260:Female; D015725:Fluconazole / Q000633:toxicity*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR
D015725:Fluconazole