[Artificial proteins with a given spatial structure and biological activity].
Biologically active fragment 131-138 of human interferon alpha 2 carrying blast-transforming activity of the protein was attached to the N-terminus of the de novo protein albebetin with predetermined tertiary structure by means of genetic engineering. The chimeric protein was expressed in a wheat germ cell-free translation system and tested for compactness, stability and biological activity. According to the tests used albebetin with interferon fragment has a compact and relatively stable structure. It binds murine thymocyte receptor with high affinity and activates efficiently thymocyte blast transformation at a concentration of 10(-11) M.
Dolgikh
D A
DA
Gabriélian
A E
AE
Navolotskaia
E V
EV
Chemeris
V V
VV
Kirpichnikov
M P
MP
D000595:Amino Acid Sequence; D001483:Base Sequence; D016898:Interferon-alpha / Q000737:chemistry*; D008969:Molecular Sequence Data; D009841:Oligonucleotides; D010446:Peptide Fragments / Q000737:chemistry*; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry; D011993:Recombinant Fusion Proteins / Q000737:chemistry; D013950:Thymus Gland / Q000737:chemistry / Q000166:cytology
D016898:Interferon-alpha; D009841:Oligonucleotides; D010446:Peptide Fragments; D011506:Proteins; D011993:Recombinant Fusion Proteins; C074146:albebetin
Iskusstvennyĭ belok s zadannoĭ prostranstvennoĭ strukturoĭ i biologicheskoĭ aktivnost'iu.