Identification of an amino acid that defines the fusogenicity of mumps virus.
Recombinant cDNA clones representing the fusion (F) and hemagglutinin-neuraminidase (HN) proteins of two mumps virus strains different in fusogenicity were constructed. Upon transfection of COS7 cells, extensive cell fusion was observed only when cells expressed the F protein of the fusing strain together with the HN protein derived from either strain. Mutational analyses further showed that the amino acid at position 195 of the F protein plays a critical role in determining the extent of cell fusion induced by mumps virus, since replacement of Ser-195 by Tyr significantly reduced the fusion inducibility of otherwise fusion-competent F protein.
Tanabayashi
K
K
Department of Viral Disease and Vaccine Control, National Institute of Health, Tokyo, Japan.
Takeuchi
K
K
Okazaki
K
K
Hishiyama
M
M
Yamada
A
A
D000818:Animals; D001483:Base Sequence; D002459:Cell Fusion; D002478:Cells, Cultured; D004252:DNA Mutational Analysis; D006389:Hemagglutinins, Viral / Q000096:biosynthesis / Q000235:genetics*; D008969:Molecular Sequence Data; D009109:Mumps virus / Q000235:genetics / Q000254:growth & development*; D011994:Recombinant Proteins; D013045:Species Specificity; D014760:Viral Fusion Proteins / Q000096:biosynthesis / Q000235:genetics*; D014779:Virus Replication
D006389:Hemagglutinins, Viral; D011994:Recombinant Proteins; D014760:Viral Fusion Proteins