Sex identification by polymerase chain reaction of alpha-satellite in aged tissue samples.
In vitro amplification of the alphoid repeated sequences clustered in the centromeric regions of the human X and Y chromosomes was carried out. A modification of the amplification conditions reported by Witt and Erickson gave clear amplification bands from 10 pg of genomic DNA template. Results were obtained from the following aged tissue samples: 1 microL three-year-old blood stain, one-year-old single hair roots, six-month-old saliva stains and twelve-year-old bone fragments. Good results were obtained even when the template was artificially fragmented into fragments of less than 1 kbp.
Fattorini
P
P
Dipartimento di Biologia, Università di Trieste, Italy.
Cacció
S
S
Gustincih
S
S
Florian
F
F
Altamura
B M
BM
Graziosi
G
G
D001483:Base Sequence; D001842:Bone and Bones / Q000737:chemistry; D004247:DNA / Q000737:chemistry / Q000302:isolation & purification; D005260:Female; D006197:Hair / Q000737:chemistry; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012091:Repetitive Sequences, Nucleic Acid; D012463:Saliva / Q000737:chemistry; D012732:Sex Determination Analysis / Q000379:methods*; D013997:Time Factors; D014960:X Chromosome; D014998:Y Chromosome
D004247:DNA