Occurrence of heterogeneity of N-linked oligosaccharides attached to sycamore (Acer pseudoplatanus L.) laccase after excretion.
The N-linked oligosaccharide moieties of sycamore (Acer pseudoplatanus L.) laccase are known to be highly heterogeneous. We confirmed that this oligosaccharide heterogeneity was caused not only during the oligosaccharide biosynthesis in Golgi apparatus, but also after the excretion of laccase protein into a culture medium. The culture medium for the sycamore cells (Acer pseudoplatanus L.) contained beta-galactosidase, alpha-L-fucosidase, beta-N-acetylglucosaminidase, alpha-mannosidase and beta-xylosidase activities. We showed that the largest sugar chain in laccase, oligosaccharide F, [formula: see text] was degraded to [formula: see text] by a crude exoglycosidase mixture in the culture medium.
Tezuka
K
K
Department of Hygienic Chemistry, Faculty of Pharmaceutical Sciences, Nagoya City University, Japan.
Hayashi
M
M
Ishihara
H
H
Onozaki
K
K
Nishimura
M
M
Takahashi
N
N
D000118:Acetylglucosaminidase / Q000378:metabolism; D002240:Carbohydrate Sequence; D002478:Cells, Cultured; D003470:Culture Media; D042845:Laccase; D008361:Mannosidases / Q000378:metabolism; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry / Q000378:metabolism*; D010088:Oxidoreductases / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D010940:Plant Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D010944:Plants / Q000201:enzymology*; D014995:Xylosidases / Q000378:metabolism; D005644:alpha-L-Fucosidase / Q000378:metabolism; D043323:alpha-Mannosidase; D001616:beta-Galactosidase / Q000378:metabolism; D001619:beta-N-Acetylhexosaminidases / Q000378:metabolism
D003470:Culture Media; D009844:Oligosaccharides; D010940:Plant Proteins; D010088:Oxidoreductases; D042845:Laccase; D008361:Mannosidases; D014995:Xylosidases; D001616:beta-Galactosidase; D043323:alpha-Mannosidase; C026579:exo-1,4-beta-D-xylosidase; D005644:alpha-L-Fucosidase; D000118:Acetylglucosaminidase; D001619:beta-N-Acetylhexosaminidases