title: Induction of GTP cyclohydrolase I by bacterial lipopolysaccharide in the rat.
abstract: A 2- to 3-fold increase of GTP cyclohydrolase I (E.C. 3.5.4.16), the key enzyme of tetrahydrobiopterin biosynthesis from GTP, was observed in cerebellum, remaining brain, liver, spleen, and adrenal gland of rats treated with a single dose of lipopolysaccharide (LPS). This led to increased biopterin levels in tissues but not in plasma. Parallel induction of nitric oxide (NO) synthase was indicated by a 10- to 100-fold increase of plasma nitrate levels 6 and 12 hours after injection of LPS. Furthermore, systolic blood pressure was reduced significantly by 23%. Our results demonstrate induction of tetrahydrobiopterin biosynthesis after LPS treatment in vivo.
authors: Institut für Medizinische Chemie und Biochemie, Universität Innsbruck, Austria.
authors: G
authors: G
authors: Werner-Felmayer
authors: H
authors: H
authors: Prast
authors: E R
authors: ER
authors: Werner
authors: A
authors: A
authors: Philippu
authors: H
authors: H
authors: Wachter
mesh_terms: D000818:Animals; D001708:Biopterins / Q000031:analogs & derivatives / Q000378:metabolism; D001794:Blood Pressure; D004790:Enzyme Induction; D006136:GTP Cyclohydrolase / Q000096:biosynthesis*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008297:Male; D009566:Nitrates / Q000097:blood; D009928:Organ Specificity; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D008070:Lipopolysaccharides; D009566:Nitrates; D001708:Biopterins; D006136:GTP Cyclohydrolase; C003402:sapropterin