title: Characterization of two African swine fever virus 220-kDa proteins: a precursor of the major structural protein p150 and an oligomer of phosphoprotein p32.
abstract: Two kinds of unrelated African swine fever virus proteins of 220 kDa have been identified by means of two-dimensional gel electrophoresis and immunoprecipitation analysis. One species, named pp220 and identified as the precursor of the major structural protein p150, was found to be a moderately acidic protein (pl near 7) expressed after the replication of the viral DNA. The second species, a cluster of 220-kDa proteins with slightly different isoelectric points (pl ranging from 5 to 6), was found to be a homooligomeric complex formed by an early 32-kDa protein. This component was identified as the viral phosphoprotein p32, the most immunogenic early protein of African swine fever virus. A detailed characterization of its oligomeric structure is reported.
authors: Centro de Biología Molecular (C.S.I.C.-U.A.M.), Facultad de Ciencias, Universidad Autónoma, Cantoblancó, Madrid, Spain.
authors: G
authors: G
authors: Andrés
authors: C
authors: C
authors: Simón-Mateo
authors: E
authors: E
authors: Viñuela
mesh_terms: D000358:African Swine Fever Virus / Q000737:chemistry*; D000818:Animals; D003432:Cross-Linking Reagents; D004220:Disulfides; D015180:Electrophoresis, Gel, Two-Dimensional; D008264:Macrophages / Q000166:cytology; D010750:Phosphoproteins / Q000096:biosynthesis; D011487:Protein Conformation; D011498:Protein Precursors / Q000302:isolation & purification*; D013552:Swine; D014709:Vero Cells; D014764:Viral Proteins / Q000096:biosynthesis / Q000302:isolation & purification*; D015678:Viral Structural Proteins / Q000096:biosynthesis
chemical_list: D003432:Cross-Linking Reagents; D004220:Disulfides; D010750:Phosphoproteins; D011498:Protein Precursors; D014764:Viral Proteins; D015678:Viral Structural Proteins; C074960:P30 protein, African Swine Fever Virus