title: Automated spectrophotometric method for determining oxidized and reduced glutathione in liver.
abstract: An enzymatic recycling method has been applied to the measurement of total and oxidized glutathione with a centrifugal analyzer. When the reduced form of glutathione (GSH) was masked with 2-vinylpyridine to measure the oxidized glutathione (GSSG), the time to ensure full derivatization was three times longer than has been reported. The method is quick, simple, accurate, and precise (1.27% for GSH, 3.3% for GSSG intraassay CV; 2.15% for GSH, 5% for GSSG interassay CV), and the automation allows large numbers of samples to be conveniently assayed.
authors: Gastrointestional Laboratory, Rayne Institute, London, UK.
authors: J P
authors: JP
authors: Teare
authors: N A
authors: NA
authors: Punchard
authors: J J
authors: JJ
authors: Powell
authors: P J
authors: PJ
authors: Lumb
authors: W D
authors: WD
authors: Mitchell
authors: R P
authors: RP
authors: Thompson
mesh_terms: D001322:Autoanalysis / Q000706:statistics & numerical data; D005978:Glutathione / Q000032:analysis*; D006801:Humans; D008099:Liver / Q000737:chemistry*; D010084:Oxidation-Reduction; D011786:Quality Control; D013053:Spectrophotometry
chemical_list: D005978:Glutathione