title: Influence of detergent aerosol on lung microvascular permeability.
abstract: Noncardiogenic edema fluid often contains high levels of plasma proteins, which may inhibit the function of the lung surfactant complex and thus decrease interstitial hydrostatic pressures. We questioned whether, in the awake and standing animal, displacement of the alveolar surface lining would alter the permeability of the thin and sparsely supported pulmonary capillaries. Sheep prepared with lung lymph fistulae were given an aerosolized detergent (Det) to displace the lung surfactant complex. After the sheep were given Det, protein flux was significantly increased (P < 0.05). To validate the suggested permeability increase, pulmonary vein occluders were surgically implanted and experiments repeated with pulmonary arterial pressures elevated 10 mmHg above baseline. After 2 h of elevated pulmonary arterial pressure, lung lymph increased fivefold. At this time, lymph-to-plasma total protein concentration ratios for air and saline-plus-ethanol vehicle were significantly lower (P < 0.01) than baseline ratios (0.26 +/- 0.06 and 0.34 +/- 0.07, respectively). No significant difference could be shown in lymph-to-plasma ratios after the sheep were given Det. We conclude that disruption of the alveolar lining can cause a detectable increase in protein flux due, in part, to an increase in microvascular permeability.
authors: Department of Surgery, University of Texas Medical Branch, Galveston 77550.
authors: C Z
authors: CZ
authors: Wang
authors: R E
authors: RE
authors: Barrow
authors: C S
authors: CS
authors: Cox
authors: S F
authors: SF
authors: Yang
authors: D N
authors: DN
authors: Herndon
mesh_terms: D000336:Aerosols; D000818:Animals; D001784:Blood Gas Analysis; D001794:Blood Pressure / Q000502:physiology; D001798:Blood Proteins / Q000378:metabolism; D002199:Capillary Permeability / Q000187:drug effects*; D003902:Detergents / Q000008:administration & dosage / Q000494:pharmacology*; D015633:Extravascular Lung Water / Q000378:metabolism; D005260:Female; D008168:Lung / Q000378:metabolism*; D008196:Lymph / Q000187:drug effects; D009929:Organ Size / Q000502:physiology; D011652:Pulmonary Circulation / Q000187:drug effects; D011654:Pulmonary Edema / Q000503:physiopathology; D011667:Pulmonary Veins / Q000187:drug effects; D012756:Sheep; D013500:Surface Tension
chemical_list: D000336:Aerosols; D001798:Blood Proteins; D003902:Detergents