Analysis of autoantibody binding to 52-kd paraneoplastic cerebellar degeneration-associated antigen expressed in recombinant proteins.
A 52-kd neural antigen was reported to be recognized by anti-Purkinje cell antibodies in serum of a patient with paraneoplastic cerebellar degeneration associated with uterine carcinoma. In this study, we demonstrate that this neural antigen is recognized by antibodies known as anti-Purkinje cell antibody type I (PCAb Type I) and anti-YO. The latter's antigen is reported to be specific for the 62- to 64-kd antigen CDR62. Assuming that the 52-kd and 62- to 64-kd antigens share a common epitope(s) recognized by all of these antibodies, we examined the antigenic region on the 52-kd protein by immunoblots with deletion fragment proteins of the recombinant 52-kd protein. A major epitope was localized in the region of amino acid residues 94 to 133 of the 52-kd protein, which is the site of a leucine zipper motif. The potential pathogenicity of PCAb Type I is discussed.
Sakai
K
K
Department of Neurology, Kanazawa Medical University, Ishikawa, Japan.
Ogasawara
T
T
Hirose
G
G
Jaeckle
K A
KA
Greenlee
J E
JE
D000595:Amino Acid Sequence; D000941:Antigens / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D001323:Autoantibodies / Q000276:immunology*; D002526:Cerebellar Diseases / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D015151:Immunoblotting; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010257:Paraneoplastic Syndromes / Q000276:immunology*; D010446:Peptide Fragments / Q000276:immunology; D011994:Recombinant Proteins
D000941:Antigens; D001323:Autoantibodies; D010446:Peptide Fragments; D011994:Recombinant Proteins