Identification of binding domains on the superantigen, toxic shock syndrome-1, for class II MHC molecules.
Toxic shock syndrome toxin-1 (TSST-1) is a member of the staphylococcal enterotoxin superantigen family. In order to determine the regions on the TSST-1 molecule involved in binding to class II MHC, seven overlapping peptides of the entire TSST-1 molecule were synthesized and tested for their ability to compete with 125I-TSST-1 for binding to class II MHC on murine A20 cells and HLA on Raji cells. Peptides corresponding to N-terminal amino acid residues 39 through 68 and C-terminal residues 155 through 194 competed with 125I-TSST-1 for binding to class II MHC. Also, binding studies with class II MHC beta-chain peptides indicate that regions encompassed by I-A beta b(30-60) and I-A beta b(60-90) are binding regions for TSST-1. Thus, we have identified binding domains on the TSST-1 molecule for class II MHC molecule receptors on antigen presenting cells.
Soos
J M
JM
Department of Microbiology and Cell Science, University of Florida, Gainesville 32611.
Russell
J K
JK
Jarpe
M A
MA
Pontzer
C H
CH
Johnson
H M
HM
D000595:Amino Acid Sequence; D000818:Animals; D000942:Antigens, Bacterial / Q000737:chemistry / Q000378:metabolism*; D001427:Bacterial Toxins; D001665:Binding Sites; D001667:Binding, Competitive; D002478:Cells, Cultured; D004768:Enterotoxins / Q000737:chemistry / Q000378:metabolism*; D000949:Histocompatibility Antigens Class II / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D051379:Mice; D008969:Molecular Sequence Data; D010455:Peptides / Q000378:metabolism; D017433:Protein Structure, Secondary; D013211:Staphylococcus aureus / Q000276:immunology; D018089:Superantigens
D000942:Antigens, Bacterial; D001427:Bacterial Toxins; D004768:Enterotoxins; D000949:Histocompatibility Antigens Class II; D010455:Peptides; D018089:Superantigens; C030107:enterotoxin F, Staphylococcal