Interactions between oestradiol and danazol on the growth of gastrointestinal tumour cells.
The gastrointestinal tumour cell lines, MKN45G and C146 possessed oestrogen receptors (ER) of affinities 1.8 x 10(-8) and 5.2 x 10(-9) M respectively. C146 and MKN45G had enhanced proliferation in the presence of oestradiol (10(-8) and 10(-10) M). Fifty-six percent (5/9) of primary gastric and colorectal tumours had their proliferation enhanced by oestradiol. The anti-steroidal drug, danazol, inhibited the basal growth of MKN45G (10 micrograms ml-1 45% of the untreated control), C146 (10 and 5 micrograms ml-1, 20 and 48% of control, respectively) and 3/9 GI primary tumours (10 micrograms ml-1). Danazol competed with 3 [H]-Oestradiol for binding to ER on MKN45G and C146, at concentrations from 10 to 1 micrograms ml-1.
Watson
S A
SA
Cancer Research Campaign Laboratories, University of Nottingham, U.K.
Crosbee
D M
DM
Dilks
K L
KL
Robertson
J F
JF
Hardcastle
J D
JD
D000971:Antineoplastic Combined Chemotherapy Protocols / Q000494:pharmacology; D001943:Breast Neoplasms / Q000188:drug therapy / Q000473:pathology / Q000648:ultrastructure; D002455:Cell Division / Q000187:drug effects; D003613:Danazol / Q000378:metabolism / Q000494:pharmacology*; D004347:Drug Interactions; D004958:Estradiol / Q000378:metabolism / Q000494:pharmacology*; D005770:Gastrointestinal Neoplasms / Q000188:drug therapy* / Q000473:pathology / Q000648:ultrastructure; D006801:Humans; D007700:Kinetics; D009376:Neoplasms, Hormone-Dependent / Q000188:drug therapy / Q000473:pathology / Q000648:ultrastructure; D011960:Receptors, Estrogen / Q000378:metabolism; D014316:Tritium
D011960:Receptors, Estrogen; D014316:Tritium; D004958:Estradiol; D003613:Danazol