Tumor cell growth arrest caused by subchromosomal transferable DNA fragments from chromosome 11.
A fundamental problem in the identification and isolation of tumor suppressor and other growth-inhibiting genes is the loss of power of genetic complementation at the subchromosomal level. A direct genetic strategy was developed to isolate subchromosomal transferable fragments (STFs) from any chromosome, each containing a selectable marker within the human DNA, that could be transferred to any mammalian cell. As a test of the method, several overlapping STFs from 11p15 were shown to cause in vitro growth arrest of rhabdomyosarcoma cells. This activity mapped between the beta-globin and insulin genes.
Koi
M
M
Howard Hughes Medical Institute, Department of Internal Medicine, University of Michigan Medical School, Ann Arbor 48109.
Johnson
L A
LA
Kalikin
L M
LM
Little
P F
PF
Nakamura
Y
Y
Feinberg
A P
AP
D000818:Animals; D001483:Base Sequence; D016466:CHO Cells; D002455:Cell Division; D002460:Cell Line; D002880:Chromosomes, Human, Pair 11; D006224:Cricetinae; D004247:DNA / Q000235:genetics*; D016147:Genes, Tumor Suppressor; D005819:Genetic Markers; D005821:Genetic Techniques; D005914:Globins / Q000235:genetics; D006801:Humans; D007328:Insulin / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D012208:Rhabdomyosarcoma / Q000473:pathology*; D014407:Tumor Cells, Cultured
D005819:Genetic Markers; D007328:Insulin; D005914:Globins; D004247:DNA