Antimetastatic activity induced by Clostridium butyricum and characterization of effector cells.
The effects of a bacterial vaccine, heat-killed Clostridium butyricum MII 588 cells, on the metastasis of B16-F10 melanoma in BDF1 mice was investigated. The vaccine stimulated natural killer (NK) cell cytotoxic activity against YAC-1 target cells, which peaked at 72 hr after the pretreatment, whereas maximum macrophage cytotoxic activity was obtained on days 9 to 11. These stimulated cytotoxic activities were also observed in B16-F10 tumor-bearing BDF1 mice. The important role of stimulated NK cells and/or macrophage in the antimetastatic effect was confirmed using Anti-asialo GM1 antibody, whole body x-ray irradiation and carrageenan treatment. In addition, the vaccine could induce a high titer of interferon-gamma (IFN-gamma), an important lymphokine which may account for a significant portion of its antimetastatic activity.
Chen
H Y
HY
National Institute of Preventive Medicine, Department of Health, Taipei, Taiwan, ROC.
Wu
S L
SL
Yeh
M Y
MY
Chen
C F
CF
Mikami
Y
Y
Wu
J S
JS
D000276:Adjuvants, Immunologic / Q000627:therapeutic use*; D000818:Animals; D001428:Bacterial Vaccines / Q000627:therapeutic use*; D002453:Cell Cycle / Q000502:physiology; D003013:Clostridium / Q000276:immunology*; D003602:Cytotoxicity, Immunologic / Q000276:immunology; D007155:Immunologic Factors / Q000627:therapeutic use*; D007167:Immunotherapy; D007371:Interferon-gamma / Q000096:biosynthesis; D007694:Killer Cells, Natural / Q000187:drug effects / Q000276:immunology*; D008262:Macrophage Activation / Q000276:immunology; D008264:Macrophages / Q000187:drug effects / Q000276:immunology; D008546:Melanoma, Experimental / Q000276:immunology / Q000556:secondary* / Q000628:therapy*; D051379:Mice; D008815:Mice, Inbred Strains; D010529:Peritoneal Cavity / Q000166:cytology; D013154:Spleen / Q000166:cytology / Q000276:immunology
D000276:Adjuvants, Immunologic; D001428:Bacterial Vaccines; D007155:Immunologic Factors; D007371:Interferon-gamma