Stability of the sperm plasma membrane of hibernating bats (Myotis velifer) compared with other mammals.
Previous experiments have established that the long-lived spermatozoa of hibernating bats are resistant to the acrosome reaction and fertilization in vitro conventional techniques. We tested the hypothesis that the membranes of these spermatozoa are more resistant to perturbation than those of other mammals. We exposed them to non-specific bilayer destabilizing agents and abrupt changes in incubation temperature and tested their response by observing their status (motility and viability) after a time interval compared with other mammals (golden hamster, rabbit, human). The results did not support the hypothesis. The inherent longevity of bat spermatozoa may thus be a function of some component other than unique resilience of their plasma membrane.
Crichton
E G
EG
Department of Molecular and Cell Biology, Penn State University, University Park 16802.
Krutzsch
P H
PH
Yanagimachi
R
R
D000818:Animals; D002462:Cell Membrane / Q000187:drug effects / Q000502:physiology; D002463:Cell Membrane Permeability / Q000187:drug effects; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D002685:Chiroptera / Q000502:physiology*; D003080:Cold Temperature; D006224:Cricetinae; D006605:Hibernation / Q000502:physiology*; D006358:Hot Temperature; D006801:Humans; D008051:Lipid Bilayers / Q000037:antagonists & inhibitors; D008297:Male; D008563:Membrane Lipids / Q000378:metabolism; D008647:Mesocricetus; D011817:Rabbits; D013045:Species Specificity; D013081:Sperm Motility / Q000187:drug effects / Q000502:physiology; D013094:Spermatozoa / Q000502:physiology*
D008051:Lipid Bilayers; D008563:Membrane Lipids