Influenza A modification of endotoxin-induced otitis media with effusion in the guinea pig.
A total of 120 Hartley strain guinea pigs were used to investigate the possible role of influenza A in endotoxin-induced otitis media with effusion. Intratympanic inoculation of 0.2 ml physiologic saline solution containing 10(4) plaque-forming units (PFU)/ml of influenza A suspension or 100 ng/ml lipopolysaccharide failed to induce either middle ear effusions or mucociliary pathologies in the tubotympanum. In contrast, intratympanic inoculation 100 ng/ml endotoxin resulted in prolonged mucociliary dysfunction and middle ear effusions when 0.2 ml 10(4) PFU/ml of influenza A was inoculated in the tympanic cavity. The inference is drawn that an influenza A infection might predispose the middle ear to endotoxin-induced otitis media with effusion.
Ohashi
Y
Y
Department of Otolaryngology, Osaka City University Medical School, Japan.
Nakai
Y
Y
Ohno
Y
Y
Sugiura
Y
Y
Okamoto
H
H
Sakamoto
H
H
Hayashi
M
M
D000818:Animals; D002923:Cilia / Q000502:physiology / Q000648:ultrastructure; D003593:Cytoplasm / Q000648:ultrastructure; D004432:Ear, Middle / Q000503:physiopathology / Q000648:ultrastructure; D004731:Endotoxins / Q000008:administration & dosage / Q000009:adverse effects*; D005064:Eustachian Tube / Q000503:physiopathology / Q000648:ultrastructure; D006168:Guinea Pigs; D009980:Influenza A virus / Q000502:physiology*; D007267:Injections; D008070:Lipopolysaccharides / Q000008:administration & dosage / Q000009:adverse effects*; D008854:Microscopy, Electron; D009079:Mucociliary Clearance / Q000502:physiology; D010034:Otitis Media with Effusion / Q000209:etiology* / Q000382:microbiology / Q000473:pathology / Q000503:physiopathology
D004731:Endotoxins; D008070:Lipopolysaccharides