title: Calcium pyrophosphate dihydrate crystal deposition in synovium. Relationship to collagen fibers and chondrometaplasia.
abstract: OBJECTIVE
  Reasons for apparent primary deposition of calcium pyrophosphate dihydrate (CPPD) crystals in some synovial membranes have not been systematically examined. We undertook the present study to investigate for and compare possible cellular and matrix factors related to the presence of these crystals in synovium and cartilage.
  
  
  METHODS
  Ten synovial membrane specimens and 6 cartilage specimens were obtained at the time of joint surgery from 10 patients with CPPD crystal deposition disease, for light microscopic (LM) and electron microscopic (EM) studies.
  
  
  RESULTS
  In all synovial and cartilage specimens, we found many of the small CPPD crystals aligned on or in parallel to collagen fibers, as seen by EM. In 9 of the 10 crystal-containing synovia, we found foci of chondrometaplasia adjacent to CPPD, by LM. In 7 of the synovia, including the one without LM evidence of chondrometaplasia, we observed the presence of chondrocyte-like cells by EM. We did not note any predictable relationship between the crystals and matrix vesicles, either in synovium or in cartilage.
  
  
  CONCLUSIONS
  Our EM findings provide evidence of the relationship of small CPPD-like crystals, presumably early forms, to collagen fibers both in synovium and in cartilage. By LM and EM, we also demonstrate evidence of a close association between chondrometaplasia and CPPD deposits in synovium. We suggest that chondrometaplasia might be responsible for synovial CPPD formation in predisposed patients. Both the collagen fibers and chondrocyte-like cells seem to be involved in the primary formation of CPPD deposits in the synovium as well as in the cartilage.
authors: Division of Rheumatology, University of Pennsylvania School of Medicine, Philadelphia.
authors: A
authors: A
authors: Beutler
authors: S
authors: S
authors: Rothfuss
authors: G
authors: G
authors: Clayburne
authors: M
authors: M
authors: Sieck
authors: H R
authors: HR
authors: Schumacher
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D002128:Calcium Metabolism Disorders / Q000378:metabolism* / Q000473:pathology; D002131:Calcium Pyrophosphate / Q000032:analysis*; D002356:Cartilage / Q000473:pathology* / Q000648:ultrastructure; D003094:Collagen / Q000648:ultrastructure; D005260:Female; D006801:Humans; D008297:Male; D008679:Metaplasia; D008854:Microscopy, Electron; D008875:Middle Aged; D013583:Synovial Membrane / Q000737:chemistry* / Q000473:pathology / Q000648:ultrastructure
chemical_list: D003094:Collagen; D002131:Calcium Pyrophosphate