title: Complex patterns of sequence variation and multiple 5' and 3' ends are found among transcripts of the erythroid ankyrin gene.
abstract: The structural protein ankyrin functions in red blood cells to link the spectrin-based membrane skeleton to the plasma membrane. Ankyrin proteins are now known to occur in most cell types, and two distinct ankyrin genes have been identified (erythroid (Ank-1) and brain (Ank-2)). We have characterized transcripts of the mouse erythroid ankyrin gene by cDNA cloning and DNA sequencing. Ank-1 transcripts of 7.5 and 9.0 kilobases are found in erythroid tissues, and a 9.0-kilobase transcript is found in cerebellum. RNA hybridization blot analysis of 13 additional mouse tissues has detected four novel Ank-1 transcripts (5.0, 3.5, 2.0, and 1.6 kilobases in size). Sequencing of Ank-1 cDNA clones isolated from mouse reticulocyte, spleen, and cerebellar libraries has identified (i) multiple 5' ends that indicate possible multiple promoters; (ii) alternative polyadenylation sites that probably account for the 7.5- and 9.0-kilobase size difference; (iii) a variety of small insertions and deletions that could produce transcripts (and ultimately proteins) of nearly identical size, but different functions; and (iv) clones with large deletions of coding sequence that account for the smaller transcripts seen in spleen, skeletal muscle, and heart.
authors: Jackson Laboratory, Bar Harbor, Maine 04609.
authors: C S
authors: CS
authors: Birkenmeier
authors: R A
authors: RA
authors: White
authors: L L
authors: LL
authors: Peters
authors: E J
authors: EJ
authors: Hall
authors: S E
authors: SE
authors: Lux
authors: J E
authors: JE
authors: Barker
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017487:Ankyrins / Q000235:genetics*; D001483:Base Sequence; D002531:Cerebellum / Q000502:physiology; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D015342:DNA Probes; D005091:Exons; D015723:Gene Library; D014644:Genetic Variation; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D009928:Organ Specificity; D016133:Polymerase Chain Reaction / Q000379:methods; D015183:Restriction Mapping; D012156:Reticulocytes / Q000502:physiology*; D017386:Sequence Homology, Amino Acid; D013154:Spleen / Q000502:physiology*; D014158:Transcription, Genetic
chemical_list: D017487:Ankyrins; D015342:DNA Probes; D004247:DNA