The hydrophilic spin trap, 5,5-dimethyl-1-pyrroline-1-oxide, does not protect the rat heart from reperfusion injury.
The role and site of free radical generation in myocardial ischemia/reperfusion injury were investigated using the hydrophilic spin trapping agent, 5,5-dimethyl-1-pyrroline-1-oxide (DMPO). DMPO (40 mM) proved ineffective in preserving the contractile performance and energy metabolism of Langendorff-perfused rat hearts following ischemia and reperfusion. This result, which is in contrast with the cardioprotection observed with hydrophobic spin traps, suggests that free radicals are generated intracellularly under these conditions.
Bradamante
S
S
CNR, Centro Sintesi e Stereochimica Speciali Sistemi Organici, Milano, Italy.
Jotti
A
A
Paracchini
L
L
Monti
E
E
Morti E [corrected to Monti
E ]
E]
D000818:Animals; D003497:Cyclic N-Oxides / Q000494:pharmacology*; D004734:Energy Metabolism / Q000187:drug effects; D005609:Free Radicals / Q000378:metabolism; D066298:In Vitro Techniques; D008297:Male; D009200:Myocardial Contraction / Q000187:drug effects; D015428:Myocardial Reperfusion Injury / Q000378:metabolism / Q000517:prevention & control*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013113:Spin Labels
D003497:Cyclic N-Oxides; D005609:Free Radicals; D013113:Spin Labels; C017245:5,5-dimethyl-1-pyrroline-1-oxide