Molecular cloning and expression of yellowfin porgy (Acanthopagrus latus houttuyn) growth hormone cDNA.
1. The growth hormone cDNA of yellowfin porgy (ypGH cDNA) consisted of 915 base pairs. 2. The deduced amino acid (aa) sequence showed that the pre-GH comprised 204 residues, of which the first 17 residues formed a signal peptide. 3. Comparison of aa sequence of ypGH to seabream, tuna, rainbow trout and chum salmon showed that ypGH shared 95.1, 94.1, 65.3 and 62.4% homology with these species, respectively. 4. By expressing the ypGH cDNA in E. coli, a polypeptide around 23 kilodaltons (kDa) was found which was immunoreactive to GH antibody.
Tsai
H J
HJ
Institute of Fisheries Science, National Taiwan University, Taipei, Republic of China.
Lin
K L
KL
Chen
T T
TT
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D003062:Codon; D004247:DNA / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D005399:Fishes / Q000235:genetics*; D015870:Gene Expression; D013006:Growth Hormone / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D010957:Plasmids; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins; D012091:Repetitive Sequences, Nucleic Acid; D012474:Salmon; D017386:Sequence Homology, Amino Acid; D014169:Transformation, Bacterial; D014337:Trout; D014413:Tuna
D003062:Codon; D011994:Recombinant Proteins; D013006:Growth Hormone; D004247:DNA