Micromethod for the determination of heparin in plasma after intravenous and subcutaneous administration.
A sensitive method has been developed for extracting and analyzing heparin from plasma after intravenous and subcutaneous administration in humans and rabbits. The glycosaminoglycans are precipitated from the biological fluid as cetylpyridinium salt, and heparin is cleaved with heparinase. The reaction products are analyzed by polyacrylamide gel electrophoresis and visualized by staining with Azure A/ammoniacal silver. With this method 12 ng of heparin can be detected.
Oreste
P
P
ITALFARMACO Research Center, Milan, Italy.
Peruzzi
M
M
Stella
P
P
D000818:Animals; D002594:Cetylpyridinium; D011232:Chemical Precipitation; D004591:Electrophoresis, Polyacrylamide Gel; D006025:Glycosaminoglycans / Q000302:isolation & purification; D006493:Heparin / Q000008:administration & dosage / Q000097:blood*; D019763:Heparin Lyase; D006801:Humans; D007275:Injections, Intravenous; D007279:Injections, Subcutaneous; D008832:Microchemistry / Q000379:methods* / Q000706:statistics & numerical data; D011133:Polysaccharide-Lyases; D011817:Rabbits; D012680:Sensitivity and Specificity
D006025:Glycosaminoglycans; D006493:Heparin; D002594:Cetylpyridinium; D011133:Polysaccharide-Lyases; D019763:Heparin Lyase