Use of trinitrobenzensulfonate for affinity labeling of lysine residues at phosphate binding sites of some enzymes.
Trinitrobenzensulfonate, a reagent for lysine residues, inactivates lamb liver 6-phosphogluconate dehydrogenase through affinity labeling. Complete inactivation is due to the binding of only one residue of reagent per enzyme subunit. Other enzymes with a phosphate binding site are also inactivated by affinity labeling. It appears that trinitrobenzensulfonate, when used at low concentrations, first binds to a phosphate binding site, then reacts with a nearby lysine residue. This reagent presents some advantages over pyridoxal phosphate, which has similar characteristics.
Hanau
S
S
Istituto di Chimica Biologica, Università, Ferrara, Italy.
Dallocchio
F
F
Rippa
M
M
D000345:Affinity Labels; D000818:Animals; D001665:Binding Sites; D007700:Kinetics; D008099:Liver / Q000201:enzymology; D008239:Lysine; D010710:Phosphates / Q000378:metabolism*; D010734:Phosphogluconate Dehydrogenase / Q000037:antagonists & inhibitors / Q000737:chemistry; D012756:Sheep; D013053:Spectrophotometry; D014302:Trinitrobenzenesulfonic Acid / Q000494:pharmacology*
D000345:Affinity Labels; D010710:Phosphates; D014302:Trinitrobenzenesulfonic Acid; D010734:Phosphogluconate Dehydrogenase; D008239:Lysine