In vitro effects of hyaluronan on prostaglandin E2 induction by interleukin-1 in rabbit articular chondrocytes.
Effects of hyaluronan (HA) on the prostaglandin E2 (PGE2) production induced by recombinant human interleukin-1 beta (rhIL-1 beta) in rabbit articular chondrocytes were studied in vitro. The rhIL-1 beta-induced PGE2 production was dose-dependently inhibited by HA. HA with the highest molecular weight (M(r) = 2.0 x 10(6)) exhibited an inhibitory effect that was statistically more significant than the same polymer of lower molecular weights (M(r) = 1.0 x 10(6), 0.5 x 10(6)). This effect was observed in both young and adult rabbit articular chondrocytes. Since PGE2 has been implicated as a mediator of inflammatory joint diseases, our observations suggest that HA may elicit an anti-inflammatory effect by inhibiting PGE2 production.
Akatsuka
M
M
Division of Biochemical Pharmacology, Maruho Co., Ltd., Osaka, Japan.
Yamamoto
Y
Y
Tobetto
K
K
Yasui
T
T
Ando
T
T
D000818:Animals; D002358:Cartilage, Articular / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D002478:Cells, Cultured; D015232:Dinoprostone / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D006820:Hyaluronic Acid / Q000494:pharmacology*; D007375:Interleukin-1 / Q000494:pharmacology*; D007719:Knee Joint; D008297:Male; D008970:Molecular Weight; D011817:Rabbits
D007375:Interleukin-1; D006820:Hyaluronic Acid; D015232:Dinoprostone