Selective alteration in B-50/GAP-43 phosphorylation in brain areas of animals characterized by cognitive impairment.
When methylazoxymethanol acetate is administered to pregnant rats at gestational day 19, the offspring are greatly impaired in the learning of a two-way active avoidance task and these behavioral changes are paralleled by a change in the phosphorylation of the protein B-50/GAP-43 in hippocampus but not in cortex. The expression of the protein is not altered, indicating that the phosphorylation of B-50 is a sensitive marker of alterations in synaptic plasticity associated with impairments of learning abilities in rats.
Di Luca
M
M
Institute of Pharmacological Sciences, University of Milan, Italy.
Merazzi
F
F
De Graan
P N
PN
Cimino
M
M
Balduini
W
W
Gispen
W H
WH
Cattabeni
F
F
D000818:Animals; D001362:Avoidance Learning / Q000187:drug effects; D001522:Behavior, Animal / Q000187:drug effects; D001923:Brain Chemistry / Q000502:physiology*; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism; D003072:Cognition Disorders / Q000139:chemically induced / Q000503:physiopathology*; D005260:Female; D019922:GAP-43 Protein; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism; D017403:In Situ Hybridization; D008562:Membrane Glycoproteins / Q000378:metabolism*; D008746:Methylazoxymethanol Acetate / Q000633:toxicity; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009473:Neuronal Plasticity / Q000187:drug effects; D010766:Phosphorylation; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley
D019922:GAP-43 Protein; D008562:Membrane Glycoproteins; D009419:Nerve Tissue Proteins; D008746:Methylazoxymethanol Acetate