Physical mapping and YAC contig analysis of the region surrounding Xist on the mouse X chromosome.
The Xist sequence has been proposed as a potential candidate for the X-inactivation center based both on its localization within the candidate region for the X-inactivation center in man and mouse and on its unique pattern of expression from the inactive X chromosome. We have cloned 550 kb of DNA surrounding the mouse Xist sequence in contiguously overlapping YAC clones and have developed a long-range restriction map that spans almost 1 Mb of this region and includes this YAC contig. The detailed restriction map we have established provides a framework for the identification of expressed sequences other than Xist that may equally exhibit unusual expression characteristics associated with X inactivation. The presence of possible structural or methylation differences within this region between the active and inactive X chromosomes has been investigated through comparative analysis of male and female genomic DNA, and we report here the identification of certain CpG-containing restriction sites around Xist that have an interesting differential methylation status on the inactive and active X chromosomes.
Heard
E
E
Unité de Génétique Moléculaire Murine, Institut Pasteur, Paris, France.
Simmler
M C
MC
Larin
Z
Z
Rougeulle
C
C
Courtier
B
B
Lehrach
H
H
Avner
P
P
D000818:Animals; D015825:Chromosomes, Fungal; D004247:DNA; D015226:Dinucleoside Phosphates / Q000378:metabolism; D004303:Dosage Compensation, Genetic; D005260:Female; D017353:Gene Deletion; D015321:Gene Rearrangement; D015698:Genomic Library; D006801:Humans; D006822:Hybrid Cells; D008297:Male; D008745:Methylation; D051379:Mice; D015183:Restriction Mapping; D014960:X Chromosome
D004247:DNA; D015226:Dinucleoside Phosphates; C015772:CpG dinucleotide