Growth hormone involvement in the regulation of tartrate-resistant acid phosphatase-positive cells that are active in cartilage and bone resorption.
Young male Sprague-Dawley rats (5-7 weeks old, 80-120 g) were hypophysectomized (HX) and maintained on thyroxin and dexamethasone replacement therapies. Ten days after surgery, some HX rats received a single injection of human growth hormone (hGH), and others five daily injections of hGH. Tartrate-resistant acid phosphatase (TRAP) histochemistry was employed in order to evaluate the number of cells of resorptive potential in the metaphyseal bone of the proximal tibiae of HX rats and was compared with normal rats and HX rats that further received hGH replacement therapy. In normal rats, two populations of TRAP-positive cells were identified: multinuclear cells, which showed histological characteristics of osteoclasts, and small mononuclear cells, the number of which was overwhelming when compared with the number of TRAP-positive multinuclear cells. Both populations were reduced in the HX rat, but more so the mononuclear cells, which were assumed to represent the precursor pool of mature osteoclasts and chondroclasts (P < 0.005). Five daily injections of hGH to HX rats brought about a significant increase in the number of TRAP-positive multinuclear cells, the number of nuclei of these cells, and the number of mononuclear TRAP-positive cells, throughout the metaphyseal bone (P < 0.05). A single injection of hGH increased only the number of TRAP-positive multinuclear cells in the trabecula/bone marrow interface (P < 0.05), indicating a very rapid fusion of precursor cells into mature osteoclasts in that particular location.(ABSTRACT TRUNCATED AT 250 WORDS)
Lewinson
D
D
Laboratory for Musculoskeletal Research, Bruce Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, Haifa.
Shenzer
P
P
Hochberg
Z
Z
D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D001862:Bone Resorption; D002356:Cartilage / Q000166:cytology / Q000187:drug effects*; D003907:Dexamethasone / Q000494:pharmacology; D013006:Growth Hormone / Q000494:pharmacology* / Q000502:physiology; D006132:Growth Plate / Q000166:cytology / Q000187:drug effects; D006651:Histocytochemistry; D007016:Hypophysectomy; D008297:Male; D010010:Osteoclasts / Q000187:drug effects* / Q000201:enzymology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013974:Thyroxine / Q000494:pharmacology; D013977:Tibia
D003907:Dexamethasone; D013006:Growth Hormone; D000135:Acid Phosphatase; D013974:Thyroxine