A method for the estimation of traces of chlorhexidine in water.
A simple method of estimating traces of chlorhexidine in water using bromothymol blue is described. The procedure involves the extraction of the chlorhexidine/bromothymol blue complex into carbon tetrachloride from a buffered neutral solution and measurement of the absorbances of the extracts in a spectrophotometer. The extracted complex has good colour stability, and the method is accurate, reproducible, and very sensitive, with concentrations of chlorhexidine down to 0.05 micrograms ml-1 measurable.
Wilson
S J
SJ
Department of Restorative Dentistry, Dental School, University of Birmingham, U.K.
Wilson
H J
HJ
D001979:Bromthymol Blue; D002251:Carbon Tetrachloride / Q000032:analysis; D002710:Chlorhexidine / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013053:Spectrophotometry / Q000379:methods; D014867:Water / Q000737:chemistry*
D014867:Water; D002251:Carbon Tetrachloride; D002710:Chlorhexidine; D001979:Bromthymol Blue