title: Characterization of T-cell clones generated from skin of patients with psoriasis.
abstract: BACKGROUND
  The infiltrating cells in psoriasis include a subpopulation of autoreactive T cells.
  
  
  OBJECTIVE
  The aim of this study was to further characterize skin-infiltrating T lymphocytes in patients with psoriasis.
  
  
  METHODS
  Forty-five T-cell clones were generated from skin biopsy specimens from two patients.
  
  
  RESULTS
  Phenotypic studies on 25 of 45 clones revealed that 19 (76%) of the clones were CD4+, 5 (20%) were CD8+, and 1 (4%) clone was CD4- and CD8-. Twenty-three clones were stained for identification of T-cell receptors. Twenty-two clones expressed alpha/beta T-cell receptors and one clone (CD4-/CD8-) expressed no T-cell receptor. Nineteen clones (42%) were autoreactive with no restriction to class I or class II HLA antigens. By contrast, proliferation of two of the seven clones was inhibited by class I monoclonal antibody, whereas proliferation of four of seven clones was inhibited by class II monoclonal antibody.
  
  
  CONCLUSIONS
  These data suggest that skin-infiltrating lymphocytes in patients with psoriasis may recognize HLA-associated molecules, perhaps the peptide of the HLA groove. The recognition of the peptide is presumably inhibited when monoclonal antibody is bound to the HLA molecule.
authors: Department of Pathology, Baylor University Medical Center, Dallas, TX 75246.
authors: A
authors: A
authors: Nikaein
authors: L
authors: L
authors: Morris
authors: C
authors: C
authors: Phillips
authors: M
authors: M
authors: Soliman
authors: G
authors: G
authors: Ordonez
authors: A
authors: A
authors: Silverman
authors: M J
authors: MJ
authors: Stone
authors: A
authors: A
authors: Menter
mesh_terms: D015704:CD4 Antigens / Q000032:analysis; D016827:CD8 Antigens / Q000032:analysis; D002999:Clone Cells / Q000276:immunology / Q000473:pathology; D006680:HLA Antigens / Q000032:analysis; D006682:HLA-DP Antigens / Q000032:analysis; D006801:Humans; D016130:Immunophenotyping; D007694:Killer Cells, Natural / Q000473:pathology; D011565:Psoriasis / Q000473:pathology*; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000032:analysis; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000032:analysis; D012867:Skin / Q000473:pathology*; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000473:pathology*
chemical_list: D015704:CD4 Antigens; D016827:CD8 Antigens; D006680:HLA Antigens; D006682:HLA-DP Antigens; D016693:Receptors, Antigen, T-Cell, alpha-beta; D016692:Receptors, Antigen, T-Cell, gamma-delta