title: Polymerase chain reaction-based point mutagenesis protocol.
authors: Department of Biochemistry and Molecular Biology, University of Kanasas Medical Center, Kansas City 66103.
authors: L J
authors: LJ
authors: Zhao
authors: Q X
authors: QX
authors: Zhang
authors: R
authors: R
authors: Padmanabhan
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D000595:Amino Acid Sequence; D001483:Base Sequence; D004274:DNA, Recombinant / Q000378:metabolism; D004279:DNA, Viral / Q000235:genetics; D004259:DNA-Directed DNA Polymerase / Q000235:genetics* / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D007202:Indicators and Reagents; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis; D010957:Plasmids; D017354:Point Mutation; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D004274:DNA, Recombinant; D004279:DNA, Viral; D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides; D004259:DNA-Directed DNA Polymerase