Effects of annexin V on the activity of the anticoagulant proteins C and S.
The effects of annexin V on the anticoagulant activity of activated protein C (APC) and protein S were examined. Although annexin V did not influence the amidolytic potential of APC, it inhibited both APC and protein S function in a factor Va inactivation assay. Competition experiments demonstrated that annexin V inhibits protein S binding to phospholipid vesicles in a dose-dependent manner. These results demonstrate that annexin V effectively interferes with the anticoagulant arm of the hemostatic system.
Sun
J
J
Department of Medicine, Monash Medical School, Box Hill Hospital, Australia.
Bird
P
P
Salem
H H
HH
D017304:Annexin A5 / Q000378:metabolism / Q000494:pharmacology*; D001777:Blood Coagulation / Q000187:drug effects*; D015943:Factor Va / Q000037:antagonists & inhibitors*; D006801:Humans; D008055:Lipids / Q000097:blood*; D008567:Membranes, Artificial; D010446:Peptide Fragments / Q000378:metabolism / Q000494:pharmacology; D010743:Phospholipids / Q000378:metabolism; D011485:Protein Binding; D011486:Protein C / Q000037:antagonists & inhibitors*; D017293:Protein S / Q000037:antagonists & inhibitors* / Q000378:metabolism; D013329:Structure-Activity Relationship
D017304:Annexin A5; D008055:Lipids; D008567:Membranes, Artificial; D010446:Peptide Fragments; D010743:Phospholipids; D011486:Protein C; D017293:Protein S; D015943:Factor Va