Flow-cytometric characterization of cardiac allograft-infiltrating cells following MHC class I antigen pretreatment in the mouse.
Changes in the graft-infiltrating cell population (GIC) induced by Class I MHC pretreatment were characterized using flow cytometric analysis. C3H mice received 10(7) EL4 cells intravenously 14 days prior to transplant with C57BL/10 (H-2b) hearts. Transplanted hearts were removed from six recipients in both groups at 4, 6, 9, 12, 15, and 21 days after transplantation. GICs were harvested and incubated with FITC-conjugated Lyt-1 and Lyt-2 and phycoerythrin-conjugated L3T4 monoclonal antibodies. The proportion of GICs which were Lyt-1, L3T4, or Lyt-2 positive was similar in both control and pretreated groups at all times posttransplant, and all three cell populations exhibited similar changes over the course of the study in both groups. At Post-transplant Day (PTD) 9, there were significantly more double-staining cells (L3T4+, Lyt2+) in the pretreated group than in the control group (8.58 +/- 2.49% vs 4.36 +/- 1.32%, P < 0.05). By PTD 15, the double-staining cells had increased in pretreated mice to 17.46 +/- 4.36% of the total GICs (P < 0.05). The percentage of GICs in EL4-pretreated mice which were Lyt-1- was significantly higher at all time points than the percentage of L3T4- or Lyt-2- cells, whereas in the control, these cell populations were equivalent, implying that in pretreated mice, cells are present which are L3T4+ or Lyt2+ but Lyt-1-.(ABSTRACT TRUNCATED AT 250 WORDS)
Bilik
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R
Department of Surgery, University of Toronto, Hospital for Sick Children, Ontario, Canada.
Superina
R A
RA
Fortner
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P
D000704:Analysis of Variance; D000818:Animals; D005260:Female; D005434:Flow Cytometry; D006085:Graft Survival / Q000276:immunology; D006183:H-2 Antigens / Q000276:immunology*; D016027:Heart Transplantation / Q000276:immunology* / Q000473:pathology; D016219:Immunotherapy, Adoptive; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D016176:T-Lymphocyte Subsets / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology* / Q000473:pathology; D013997:Time Factors; D014184:Transplantation, Homologous / Q000276:immunology; D014407:Tumor Cells, Cultured
D006183:H-2 Antigens