Cell-attachment activities of surface immobilized oligopeptides RGD, RGDS, RGDV, RGDT, and YIGSR toward five cell lines.
Tetrapeptides, Arg-Gly-Asp-Ser (RGDS), Arg-Gly-Asp-Val (RGDV), and Arg-Gly-Asp-Thr (RGDT), respectively, appearing in the cell-attachment domains of fibronectin, vitronectin, and collagen, and pentapeptide Tyr-Ile-Gly-Ser-Arg (YIGSR) appearing in B1 chain of laminin, were synthesized by liquid-phase procedure. Bioactivities of RGD, RGDX (X = S, V and T), YIGSR, and YIGSR-NH2 as cell recognition determinants were investigated by cell-attachment test using these oligopeptides immobilized to ethylene-acrylic acid copolymer (PEA) film. The cell lines used were A431, NRK, CHO-K1, HeLa.S3, and RLC-16 cells. It was found that the residue X in RGDX plays an important role for cell-attachment activity of RGDX, and, regarding YIGSR, introduction of NH2 residue at the C-terminal of the pentapeptide enhances the cell-attachment activity.
Hirano
Y
Y
Department of Applied Chemistry, Osaka Institute of Technology, Japan.
Okuno
M
M
Hayashi
T
T
Goto
K
K
Nakajima
A
A
D000180:Acrylic Resins; D000595:Amino Acid Sequence; D000818:Animals; D001672:Biocompatible Materials; D002448:Cell Adhesion / Q000187:drug effects*; D002460:Cell Line; D006224:Cricetinae; D006801:Humans; D008422:Materials Testing; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000737:chemistry / Q000494:pharmacology*; D011095:Polyethylenes; D051381:Rats; D013329:Structure-Activity Relationship; D013499:Surface Properties
D000180:Acrylic Resins; D001672:Biocompatible Materials; D009842:Oligopeptides; D011095:Polyethylenes; C006903:carbopol 940; C047981:arginyl-glycyl-aspartic acid; C046617:arginyl-glycyl-aspartyl-serine