Pharmacological characterization and autoradiographic localization of dopamine D1 receptors in the human umbilical artery.
Combined in vitro radioreceptor binding and autoradiographic techniques with [3H]SCH 23390 as a ligand were used to analyze the pharmacological profile and anatomical localization of dopamine D1 receptor sites in sections of human umbilical artery. The ligand was bound to sections of the artery in a manner consistent with the labelling of D1 receptors. These receptors, which show a fetal-to-maternal gradient with the highest concentration near the placenta, are located within the smooth muscle layer of the umbilical artery. The above findings suggest that endogenous dopamine may be involved in the control of the tone of the umbilical artery through the interaction with D1 receptors.
Ferreira-de-Almeida
J A
JA
Clinica Obstetrica, Faculdade de Medicina do Porto, Portugal.
Pereira-Leite
L
L
Cavallotti
C
C
Ricci
A
A
Amenta
F
F
D000328:Adult; D001345:Autoradiography; D001552:Benzazepines; D001667:Binding, Competitive / Q000187:drug effects; D005260:Female; D006801:Humans; D009129:Muscle Tonus / Q000187:drug effects; D009131:Muscle, Smooth, Vascular / Q000033:anatomy & histology / Q000187:drug effects / Q000378:metabolism; D011247:Pregnancy; D011869:Radioligand Assay; D017447:Receptors, Dopamine D1 / Q000187:drug effects*; D014469:Umbilical Arteries / Q000033:anatomy & histology* / Q000187:drug effects* / Q000378:metabolism
D001552:Benzazepines; D017447:Receptors, Dopamine D1