The ability of murine leukocytes to bind lipocortin 1 is lost during acute inflammation.
Lipocortin 1, a member of the annexin superfamily of calcium and phospholipid binding proteins, mediates some of the anti-inflammatory actions of the glucocorticoid hormones. Lipocortin 1 binds to the surface of murine peripheral blood monocytes and polymorphonuclear leukocytes (Kd estimate 2 x 10(-8) M) but not lymphocytes. Resident peritoneal macrophages exhibit binding (Kd estimate 1.3 x 10(-8) M) but lymphocytes do not. A 95-98% reduction in lipocortin 1 binding was observed to leukocytes obtained from air pouch or peritonitis models of inflammation. When given intravenously, lipocortin 1 binds rapidly to murine leukocytes within 5 min but disappears before 10 min, leaving the binding capacity of the cells unaltered. Modulation of lipocortin 1 binding sites could be an important step in regulating the function of inflammatory cells.
Perretti
M
M
Department of Biochemical Pharmacology, William Harvey Research Institute, London, UK.
Flower
R J
RJ
Goulding
N J
NJ
D000818:Animals; D017305:Annexin A1 / Q000378:metabolism*; D006801:Humans; D007249:Inflammation / Q000276:immunology*; D007962:Leukocytes / Q000378:metabolism*; D008297:Male; D051379:Mice; D010538:Peritonitis / Q000276:immunology
D017305:Annexin A1