title: Erythroid transcription factor GATA-1 is abundantly transcribed in mouse testis.
abstract: The transcription factor GATA-1 is a fundamental regulator of genes in haematopoietic cell lineages and belongs to a family of factors that bind to the consensus sequence WGATAR. The GATA motif was originally identified in cis-regulatory regions of globin and other erythroid-specific genes, but the range of genes controlled by the GATA factors has since expanded. Members of the GATA transcription factor family share a conserved zinc-finger DNA-binding domain, but the expression profile of each GATA factor is distinct. Here we show that a testis form of murine (m)GATA-1 messenger RNA is transcribed from a promoter located 5' to the erythroid first exon, and the remaining exons (which encode the mGATA-1 protein) are used in common by both testis and erythroid transcripts. We use an anti-mGATA-1 monoclonal antibody to show that the factor expressed in erythroid cells is the same as that found in the seminiferous tubules of murine testis. The GATA-1-expressing cells in 10-week-old testis were found only in contact with the basement membrane of seminiferous tubules, suggesting that GATA-1 regulates genes during the earliest stages of spermatogenesis.
authors: Department of Pediatrics, Hirosaki University School of Medicine, Japan.
authors: E
authors: E
authors: Ito
authors: T
authors: T
authors: Toki
authors: H
authors: H
authors: Ishihara
authors: H
authors: H
authors: Ohtani
authors: L
authors: L
authors: Gu
authors: M
authors: M
authors: Yokoyama
authors: J D
authors: JD
authors: Engel
authors: M
authors: M
authors: Yamamoto
mesh_terms: D000375:Aging / Q000502:physiology; D000818:Animals; D001483:Base Sequence; D004268:DNA-Binding Proteins / Q000235:genetics*; D050981:Erythroid-Specific DNA-Binding Factors; D050982:GATA1 Transcription Factor; D015870:Gene Expression; D015723:Gene Library; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D009928:Organ Specificity; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D012689:Sequence Homology, Nucleic Acid; D013737:Testis / Q000166:cytology / Q000254:growth & development / Q000502:physiology*; D014157:Transcription Factors / Q000235:genetics*; D014158:Transcription, Genetic; D016335:Zinc Fingers / Q000235:genetics
chemical_list: D004268:DNA-Binding Proteins; D050981:Erythroid-Specific DNA-Binding Factors; D050982:GATA1 Transcription Factor; C494689:Gata1 protein, mouse; D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger; D014157:Transcription Factors