Facilitation of [3H]-ACh release by forskolin depends on A2-adenosine receptor activation.
The effect of forskolin (FSK) on [3H]-acetylcholine release ([3H]-ACh) from the phrenic motor nerve terminals, and its modification by adenosine deaminase (ADA), by the A2-adenosine receptor agonist 2-[p-(2-carboxyethyl)phenethylamino]-5'-N-ethylcarboxamide adenosine (CGS 21680C), by the A1-adenosine receptor agonist R-N6-phenylisopropyl adenosine (R-PIA), by the A2-antagonist N-(2-(dimethylamino)-ethyl)-N-methyl-4-(2,3,6,7-tetrahydro-2,6-dioxo-1,3 -dipropyl-1H-purine-8-yl)-benzene sulphonamide (PD 115,199), and by the A1-antagonist 1,3-dipropyl-8-cyclopentylxanthine (DPCPX) were studied on the rat phrenic-hemidiaphragm preparation. It is concluded that the excitatory effect of FSK on evoked [3H]-ACh release depends on tonic A2-adenosine receptor activation.
Correia-de-Sá
P
P
Laboratory of Pharmacology, ICBAS, University of Oporto, Portugal.
Ribeiro
J A
JA
D000109:Acetylcholine / Q000378:metabolism*; D000241:Adenosine / Q000031:analogs & derivatives / Q000494:pharmacology; D000243:Adenosine Deaminase / Q000378:metabolism; D000262:Adenylyl Cyclases / Q000378:metabolism; D000818:Animals; D005576:Colforsin / Q000494:pharmacology*; D003964:Diaphragm / Q000166:cytology / Q000294:innervation; D005260:Female; D066298:In Vitro Techniques; D008297:Male; D009411:Nerve Endings / Q000187:drug effects / Q000378:metabolism; D010627:Phenethylamines / Q000494:pharmacology; D010660:Phenylisopropyladenosine / Q000494:pharmacology; D010791:Phrenic Nerve / Q000166:cytology; D058914:Purinergic Antagonists; D011687:Purines / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D011983:Receptors, Purinergic / Q000187:drug effects*; D013449:Sulfonamides / Q000494:pharmacology; D014970:Xanthines / Q000494:pharmacology; D000096926:Benzenesulfonamides
D000109:Acetylcholine; D000241:Adenosine; D000243:Adenosine Deaminase; D000262:Adenylyl Cyclases; D005576:Colforsin; D010627:Phenethylamines; D010660:Phenylisopropyladenosine; D058914:Purinergic Antagonists; D011687:Purines; D011983:Receptors, Purinergic; D013449:Sulfonamides; D014970:Xanthines; C052023:N-(2-(dimethylamino)ethyl)-N-methyl-4-(2,3,6,7-tetrahydro-2,6-dioxo-1,3-dipropyl-1H-purin-8-yl)benzenesulfonamide; D000096926:Benzenesulfonamides; C061282:2-(4-(2-carboxyethyl)phenethylamino)-5'-N-ethylcarboxamidoadenosine; C051360:1,3-dipropyl-8-cyclopentylxanthine