Epidermal growth factor limits structural alterations in gastrointestinal tissues and decreases bacterial translocation in burned mice.
BACKGROUND
Burn injury produces acute gastrointestinal derangements that may predispose to bacterial translocation (BT). We studied effects of recombinant human epidermal growth factor (r-HuEGF), a gastrointestinal trophic hormone, on gastrointestinal alterations and BT after murine burn injury.


METHODS
r-HuEGF was administered 1 and 12 hours after burn injury in a dose of 4 micrograms per animal subcutaneously after 25% and 32% total body surface area (TBSA) scald burn. Small bowel and gastric weight and histologic factors were studied, and BT was measured by culturing mesenteric lymph nodes.


RESULTS
Mice treated with r-HuEGF maintained gastric and small intestine weight measured 24 hours after burn injury, and ileal mucosal height was preserved, whereas burned-untreated mice lost organ weight and mucosal height. BT was decreased significantly in mice with 32% TBSA burn injury treated with r-HuEGF after injury (burn, 64.2% of animals had BT; burn-r-HuEGF, 34.6% had BT; p < 0.05). After 25% TBSA burn injury, BT was also decreased in r-HuEGF-treated animals (burn, 31.4% of animals had BT; burn-r-HuEGF, 14.3% had BT), but the difference was not statistically significant (p < 0.1).


CONCLUSIONS
r-HuEGF improves intestinal and gastric structure in mice 24 hours after burn injury and decreases BT after 32% TBSA burn injury.
Zapata-Sirvent
R L
RL
Department of Surgery, University of California-San Diego Medical Center 92103.
Hansbrough
J F
JF
Wolf
P
P
Grayson
L S
LS
Nicolson
M
M
D000042:Absorption; D000818:Animals; D001419:Bacteria / Q000187:drug effects; D018407:Bacterial Physiological Phenomena; D002056:Burns / Q000382:microbiology* / Q000473:pathology*; D004064:Digestive System / Q000187:drug effects / Q000473:pathology*; D004815:Epidermal Growth Factor / Q000493:pharmacokinetics / Q000494:pharmacology*; D005260:Female; D007413:Intestinal Mucosa / Q000187:drug effects / Q000473:pathology; D051379:Mice; D009929:Organ Size; D011994:Recombinant Proteins
D011994:Recombinant Proteins; D004815:Epidermal Growth Factor