Increasing the chemical potential of the germ-line antibody repertoire.
To augment the chemical potential of the immunological repertoire, a metal ion-binding light chain has been introduced into the murine genome. Mice containing the transgene were subsequently immunized with a fluorescein conjugate. The transgenic light chain was found at a high frequency in the anti-fluorescein memory B-cell compartment. This general method should be applicable to other cofactors and small molecules and should lead to generation of antibodies with unique catalytic activities.
Sarvetnick
N
N
Department of Neuropharmacology, Scripps Research Institute, La Jolla, CA 92037.
Gurushanthaiah
D
D
Han
N
N
Prudent
J
J
Schultz
P
P
Lerner
R
R
D000818:Animals; D000917:Antibody Formation; D001402:B-Lymphocytes / Q000276:immunology*; D001483:Base Sequence; D003433:Crosses, Genetic; D005091:Exons; D006825:Hybridomas / Q000276:immunology; D007145:Immunoglobulin kappa-Chains / Q000235:genetics* / Q000378:metabolism*; D008099:Liver / Q000276:immunology; D051379:Mice; D008815:Mice, Inbred Strains; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009838:Oligodeoxyribonucleotides; D016133:Polymerase Chain Reaction / Q000379:methods; D015183:Restriction Mapping; D013154:Spleen / Q000276:immunology; D014407:Tumor Cells, Cultured
D007145:Immunoglobulin kappa-Chains; D009838:Oligodeoxyribonucleotides