Effects of stonefish (Synanceia trachynis) venom on murine and frog neuromuscular junctions.
The neuromuscular toxicity of stonefish (Synanceia trachynis) venom was characterized by electrophysiological and electron microscopic examination of isolated murine and frog nerve-skeletal muscle preparations exposed to various concentrations of venom. Low concentrations of venom (2.5-10 micrograms/ml) acted presynaptically by causing release and depletion of neurotransmitter from the nerve terminal. The response was Na+ channel-independent (resistant to tetrodotoxin), required the presence of either Ca2+ or Mg2+, and was observed with botulinum neurotoxin-paralyzed nerve-muscle preparations. Higher concentrations of venom (100-300 micrograms/ml) acted postsynaptically and presynaptically. They caused irreversible depolarization of muscle cells and microscopically observable muscle and nerve damage. We conclude that the previously observed neuromuscular toxicity of stonefish venom is a consequence of the venom's dose-dependent, presynaptic and postsynaptic actions at the myoneural junction.
Kreger
A S
AS
Medical Sciences Research Institute, Herndon, Virginia 22071.
Molgó
J
J
Comella
J X
JX
Hansson
B
B
Thesleff
S
S
D000109:Acetylcholine / Q000378:metabolism; D000818:Animals; D001905:Botulinum Toxins / Q000494:pharmacology; D002462:Cell Membrane / Q000187:drug effects / Q000648:ultrastructure; D004594:Electrophysiology; D005397:Fish Venoms / Q000494:pharmacology*; D005399:Fishes / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D008854:Microscopy, Electron; D009045:Motor Endplate / Q000187:drug effects / Q000648:ultrastructure; D009132:Muscles / Q000473:pathology; D009411:Nerve Endings / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D009467:Neuromuscular Depolarizing Agents / Q000494:pharmacology; D009469:Neuromuscular Junction / Q000187:drug effects* / Q000648:ultrastructure; D011893:Rana esculenta; D051381:Rats; D017207:Rats, Sprague-Dawley; D013569:Synapses / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure
D005397:Fish Venoms; D009467:Neuromuscular Depolarizing Agents; D001905:Botulinum Toxins; D000109:Acetylcholine