Monoclonal anti-biotin antibodies simulate avidin in the recognition of biotin.
The sequence of the VH gene of a monoclonal anti-biotin antibody was determined. Biotin-binding motifs, similar to those in avidin and streptavidin, were identified in complementary determining regions 2 and 3, suggesting that natural selection of functional motifs may occur in unrelated protein types.
Bagçi
H
H
Department of Hormone Research, Weizmann Institute of Science, Rehovot, Israel.
Kohen
F
F
Kusçuoglu
U
U
Bayer
E A
EA
Wilchek
M
M
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000235:genetics / Q000276:immunology*; D000915:Antibody Affinity; D001360:Avidin / Q000378:metabolism*; D001426:Bacterial Proteins / Q000737:chemistry; D001483:Base Sequence; D001665:Binding Sites; D001710:Biotin / Q000276:immunology* / Q000378:metabolism; D004247:DNA; D005260:Female; D007143:Immunoglobulin Heavy Chains / Q000737:chemistry / Q000235:genetics*; D007135:Immunoglobulin Variable Region / Q000737:chemistry / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D019809:Streptavidin
D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins; D007143:Immunoglobulin Heavy Chains; D007135:Immunoglobulin Variable Region; D001360:Avidin; D001710:Biotin; D004247:DNA; D019809:Streptavidin