[Cloning and sequencing of cDNA of the hepatitis E virus genome--application to diagnosis].
Hepatitis E is endemic, often provoking epidemics in many developing countries. The hepatitis E virus has been molecularly cloned and sequenced and has a single-stranded, positive-sense RNA genome, 7,194 nucleotides, followed by a poly(A) tail. There are three open reading frames. The non-structural gene, approximately 5 kb, is located at the 5' end, while the structural gene, approximately 2 kb, is located at the 3' end of the genome. There is low level nucleotide variation among hepatitis E virus strains isolated from Myanmar and China.
Uchida
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Department of Pathology I, Nihon University School of Medicine.
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004279:DNA, Viral; D005814:Genes, Viral; D014644:Genetic Variation; D016679:Genome, Viral; D016751:Hepatitis E / Q000175:diagnosis* / Q000453:epidemiology; D016752:Hepatitis E virus / Q000235:genetics*; D006801:Humans
D004279:DNA, Viral