title: Use of Laurdan fluorescence in studying plasma membrane organization of polymorphonuclear leukocytes during the respiratory burst.
abstract: The changes in plasma membrane polarity of polymorphonuclear leukocytes (PMN) during the activation of the respiratory burst were investigated by measuring the steady-state fluorescence emission spectra of 2-dimethylamino(6-lauroyl) naphthalene (Laurdan), which is known to be incorporated at the hydrophobic-hydrophilic interface of the bilayer, displaying spectral sensitivity to the polarity of its surroundings. Laurdan shows a marked steady-state emission blue shift in nonpolar solvents, with respect to polar solvents. Our results show a blue shift of the fluorescence emission spectra of Laurdan during activation of PMN with phorbol myristate acetate or N-formyl-methionyl-leucyl-phenylalanine. These results suggest that the activation of the respiratory burst of PMN is accompanied by a decrease in polarity in the hydrophobic-hydrophilic interface of the plasma membrane.
authors: Institute of Biochemistry, University of Ancona, Italy.
authors: R
authors: R
authors: Fiorini
authors: G
authors: G
authors: Curatola
authors: A
authors: A
authors: Kantar
authors: P L
authors: PL
authors: Giorgi
authors: E
authors: E
authors: Gratton
mesh_terms: D015081:2-Naphthylamine / Q000031:analogs & derivatives; D002462:Cell Membrane / Q000502:physiology* / Q000648:ultrastructure; D005456:Fluorescent Dyes; D006801:Humans; D007848:Laurates; D008163:Luminescent Measurements; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D010101:Oxygen Consumption / Q000187:drug effects; D013050:Spectrometry, Fluorescence / Q000379:methods; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: D005456:Fluorescent Dyes; D007848:Laurates; D009240:N-Formylmethionine Leucyl-Phenylalanine; D015081:2-Naphthylamine; D013755:Tetradecanoylphorbol Acetate; C065580:laurdan