title: A synthetic opioid peptide increases plasma growth hormone and prolactin in Holstein calves.
abstract: The effect of the synthetic opioid agonist D-Ala2,N-Me-Phe4,Met(O)5-ol enkephalin (DAMME) on plasma growth hormone (GH) and prolactin (PRL) concentrations in Holstein heifer calves was investigated in this study. The possible site of action of DAMME was determined by pretreating calves with an opioid antagonist that crosses the blood-brain barrier poorly if at all (N-methyl levallorphan-methane sulphonate [MLM]) or one that crosses readily (naloxone [NAL]). All calves were assigned to one of three treatment groups: 1) pretreatment with saline, 2) pretreatment with NAL, or 3) pretreatment with MLM. All groups were injected with DAMME 30 min after pretreatments. Plasma PRL increased after injection of DAMME in calves pretreated with saline. Prolactin concentrations were not different before and after injection of DAMME in calves pretreated with either NAL or MLM. Plasma GH increased after injection of DAMME in saline- and MLM-pretreated calves but was unchanged in NAL-pretreated calves. These data show that peripherally administered DAMME increases plasma GH and PRL in Holstein heifer calves and suggest that DAMME mediates GH release through receptors located somewhere inside the blood-brain barrier, but it can induce PRL secretion at a site located outside the barrier.
authors: Department of Dairy Science, Virginia Polytechnic Institute and State University, Blacksburg 24061.
authors: D W
authors: DW
authors: Johnson
authors: M A
authors: MA
authors: Barnes
authors: R M
authors: RM
authors: Akers
authors: R E
authors: RE
authors: Pearson
mesh_terms: D000818:Animals; D001812:Blood-Brain Barrier; D002417:Cattle / Q000378:metabolism*; D005405:D-Ala(2),MePhe(4),Met(0)-ol-enkephalin / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000494:pharmacology*; D005260:Female; D013006:Growth Hormone / Q000097:blood*; D007275:Injections, Intravenous; D016018:Least-Squares Analysis; D007977:Levallorphan / Q000031:analogs & derivatives / Q000494:pharmacology; D009270:Naloxone / Q000493:pharmacokinetics / Q000494:pharmacology; D009292:Narcotic Antagonists / Q000494:pharmacology; D011388:Prolactin / Q000097:blood*; D011897:Random Allocation
chemical_list: D009292:Narcotic Antagonists; D007977:Levallorphan; D009270:Naloxone; C045482:N-methyllevallorphan; D005405:D-Ala(2),MePhe(4),Met(0)-ol-enkephalin; D011388:Prolactin; D013006:Growth Hormone