[Rec41--a new gene, participating in the control of recombination in Saccharomyces cerevisiae yeast].
We isolated a collection of rec- mutants. The mutant rec41 from this collection was studied in detail. The mutant demonstrated a reduced level of interplasmid recombination, did not grow at the elevated temperature (36 degrees C) and was sensitive to gamma-rays but not to ultraviolet irradiation. The rec41-1 mutation behaved as a semidominant nuclear mutation. Our study on inter- and intraplasmid recombination suggested that the rec41-1 mutation decreased the level of crossing over events significantly and the frequency of gene conversion events at a lower degree. We postulate that rec41 mutation reduces the efficiency of double-stranded break repair and this results in high sensitivity of mutant cells to methylenmethan-sulfonate and ionizing radiation. Using gamma-sensitivity of the mutant cells we isolated a rec41-1 complementing plasmid from a yeast genomic DNA library. The mapping of plasmid integration site allowed us to localize the REC41 gene on the left arm of the VII chromosome nearby LYS4 and CDC43 genes.
Chepurnaia
O V
OV
Kozhina
T N
TN
Peshekhonov
V T
VT
Korolev
V G
VG
D003001:Cloning, Molecular; D004249:DNA Damage; D004271:DNA, Fungal / Q000528:radiation effects; D005656:Fungal Proteins / Q000235:genetics*; D005800:Genes, Fungal; D016296:Mutagenesis; D009154:Mutation; D010957:Plasmids; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000528:radiation effects
D004271:DNA, Fungal; D005656:Fungal Proteins
Rec41--novyĭ gen, uchastvuiushchiĭ v kontrole rekombinatsii u drozhzheĭ Saccharomyces cerevisiae.