Increased projection of MHC and tumor antigens in murine B16-BL6 melanoma induced by hydrostatic pressure and chemical crosslinking.
The B16-BL6 melanoma, like most spontaneously arising tumors, is poorly immunogenic and expresses low levels of major histocompatibility complex (MHC) antigens. Treatment of cells of this tumor in vitro by hydrostatic pressure in the presence of adenosine 2',3'-dialdehyde (oxAdo), a membrane-impermeant crosslinker, caused elevated projection of MHC and a specific tumor antigen as demonstrated by flow-cytometric analysis. Maximum projection of both the MHC and the tumor antigens could be reached by application of 1200 atm for 15 min in the presence of 20 mM oxAdo. It is not yet clear whether this passive increase in availability of antigens on the cell surface originated from a dormant pool of antigens in the plasma membrane or from pressure-induced fusion of antigen-rich intracellular organelles (e.g. the endoplasmic reticulum). The immunogenic properties of the antigen-enriched B16-BL6 cells are described in the following paper.
Ramakrishna
V
V
Department of Membrane Research and Biophysics, Weizmann Institute of Science, Rehovot, Israel.
Eisenthal
A
A
Skornick
Y
Y
Shinitzky
M
M
D000818:Animals; D000951:Antigens, Neoplasm / Q000737:chemistry / Q000276:immunology*; D000954:Antigens, Surface / Q000276:immunology; D003432:Cross-Linking Reagents; D005260:Female; D006183:H-2 Antigens / Q000276:immunology*; D006874:Hydrostatic Pressure; D007371:Interferon-gamma / Q000494:pharmacology; D008285:Major Histocompatibility Complex; D008297:Male; D008546:Melanoma, Experimental / Q000276:immunology*; D051379:Mice; D008815:Mice, Inbred Strains; D011994:Recombinant Proteins
D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D003432:Cross-Linking Reagents; D006183:H-2 Antigens; D011994:Recombinant Proteins; D007371:Interferon-gamma