[The prospects for the development and use in ecological research of a cytogenetic method of analysing micronuclei in hepatocytes].
The article deals with peculiarities of hepatocyte proliferation which determine the fields of uses for a micronuclear test in hepatocytes and first of all for ecological biomonitoring of genotoxic pollution. Theoretical problems are considered that substantiate applied practices for performing the micronuclear test. Among them there are methods of stimulating the mitotic activity (partial resection of liver, necrogenic hepatotoxins, chemical inductors of adaptive growth); effects of cellular kinetics (volume of proliferative pool, blockade of premitotic period) on quantitative criteria of the method. The capacity of hepatocyte population to preserve and accumulate injuries of genetic material is discussed.
Uryvaeva
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D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002869:Chromosome Aberrations; D004784:Environmental Monitoring / Q000379:methods*; D004785:Environmental Pollutants / Q000633:toxicity; D008099:Liver / Q000187:drug effects / Q000648:ultrastructure*; D008115:Liver Regeneration / Q000187:drug effects; D051379:Mice; D015162:Micronucleus Tests / Q000379:methods*; D008938:Mitosis / Q000187:drug effects; D051381:Rats
D004785:Environmental Pollutants
Perspektivy razrabotki i primeneniia v ékologicheskikh issledovaniiakh tsitogeneticheskogo metoda analiza mikroiader v gepatotsitakh.