Inward potassium rectifier current in type I vestibular hair cells isolated from guinea pig.
Large inward current activated by hyperpolarization was studied using whole cell patch clamp technique in type I vestibular hair cells of guinea pig. Near the resting membrane potential, at an holding potential of -60 mV (HP -60), this current increased with hyperpolarizing steps and showed time-dependent decay for steps below -80 mV. This current was progressively inactivated at more negative holding potential and was totally abolished at HP -90 mV. The underlying conductance was a K+ conductance as indicated by: (i) its dependence on the external potassium concentration; (ii) its tail currents, which reversed at about -90 mV in solutions with a normal gradient for K+ ions. Pharmacological studies revealed that external application of 4-aminopyridine (5 mM) reversibly blocked (95%) the total inward current, while external application of tetraethylammonium (10 mM) or cesium (2 mM) did not significantly affect the amplitude of this current. This potassium inward rectifier current could contribute to restoration of the resting membrane potential during negative stimulations.
Griguer
C
C
Laboratoires de Neurophysiologie cellulaire et sensorielle, INSERM U-254, Université Montpellier II, France.
Sans
A
A
Valmier
J
J
Lehouelleur
J
J
D015761:4-Aminopyridine / Q000494:pharmacology; D000818:Animals; D002469:Cell Separation; D004594:Electrophysiology; D006168:Guinea Pigs; D006198:Hair Cells, Auditory / Q000187:drug effects / Q000502:physiology*; D011188:Potassium / Q000037:antagonists & inhibitors / Q000502:physiology*
D015761:4-Aminopyridine; D011188:Potassium