title: Developmental expression of the gastrin and cholecystokinin genes in rat colon.
abstract: BACKGROUND
  To elucidate the hypothesis that gastrin and cholecystokinin (CCK) are local growth factors for colorectal mucosa, we have examined the peptide gene expression in rat colon during development.
  
  
  METHODS
  Northern analysis, reverse transcription PCR, and sequence-specific radioimmunoassays were the essential methods.
  
  
  RESULTS
  High concentrations of gastrin and CCK messenger RNA were found in the fetal colon. At birth, gastrin and CCK mRNA's were both undetectable but increased subsequently towards adult life. The fetal colon contained 5.5 and 4.2 pmol/g tissue gastrin and CCK, respectively. After birth, carboxyamidated gastrin disappeared from the colon, whereas the concentration of CCK remained at 1 pmol/g. Glycine-extended gastrin and CCK were also present in the fetal colon, but towards adult life they decreased below 0.2 pmol/g. In contrast, progastrin and proCCK were detectable at all ages.
  
  
  CONCLUSIONS
  Rat colon expresses the gastrin and CCK genes throughout life. The posttranslational maturation of progastrin, however, ceases shortly after birth, indicating that gastrin may play a role in the developing colon. Whether CCK influences the development remains to be shown.
authors: Department of Clinical Biochemistry, Rigshospitalet, University of Copenhagen, Denmark.
authors: H R
authors: HR
authors: Lüttichau
authors: W W
authors: WW
authors: Van Solinge
authors: F C
authors: FC
authors: Nielsen
authors: J F
authors: JF
authors: Rehfeld
mesh_terms: D000375:Aging / Q000235:genetics*; D000818:Animals; D000831:Animals, Newborn; D015152:Blotting, Northern; D002766:Cholecystokinin / Q000235:genetics* / Q000378:metabolism; D003106:Colon / Q000254:growth & development / Q000502:physiology*; D005260:Female; D005333:Fetus; D005755:Gastrins / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation; D009838:Oligodeoxyribonucleotides; D016133:Polymerase Chain Reaction / Q000379:methods; D011247:Pregnancy; D011498:Protein Precursors / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D015347:RNA Probes; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D014158:Transcription, Genetic
chemical_list: D005755:Gastrins; D009838:Oligodeoxyribonucleotides; D011498:Protein Precursors; D015347:RNA Probes; D012333:RNA, Messenger; C013706:big gastrin; C060249:procholecystokinin; D002766:Cholecystokinin