Arginine-vasopressin increases the release of ET-1 into perfusate of rat mesenteric artery.
The regulatory systems of endogenous endothelin-1 (ET-1) production from intact tissues and the effects of produced ET-1 on vascular tonus by a closed circuit perfusion system of rat mesenteric artery were investigated. It was demonstrated that ET-1 is released from intact mesenteric arterial beds from Wistar rats both under basal conditions and after stimulation with arginine-vasopressin (AVP) (10(-10)-10(-9) M). Furthermore, AVP (10(-10)-10(-9) M) markedly and dose-dependently induced the expression of prepro ET-1 mRNA in the mesenteric arterial beds. Increased release of ET-1 by AVP may contribute to maintaining vascular tonus. Concomitant addition of actinomycin D inhibited the increased expression of prepro ET-1 mRNA and reduced the amount of immunoreactive ET-1 found during the 6-hour perfusate from AVP (10(-10) M)-stimulated and -unstimulated tissues.
Tomobe
Y
Y
Department of Pharmacology, University of Tsukuba, Ibaraki, Japan.
Yanagisawa
M
M
Fujimori
A
A
Masaki
T
T
Goto
K
K
D000818:Animals; D001127:Arginine Vasopressin / Q000494:pharmacology*; D016232:Endothelins / Q000235:genetics / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D008638:Mesenteric Arteries / Q000187:drug effects* / Q000378:metabolism; D010477:Perfusion; D011498:Protein Precursors / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
D016232:Endothelins; D011498:Protein Precursors; D012333:RNA, Messenger; D001127:Arginine Vasopressin