title: Purification and some properties of peroxidase isozymes from pineapple stem.
abstract: The enzyme peroxidase is widely distributed among the higher plants. Isozymes of peroxidase are known to occur in a variety of tissues in a large number of plant species. In this study, peroxidase isozymes were purified from the extract of pineapple stem through successive steps of ammonium sulfate fractionation, CM-Sepharose CL-6B chromatographies and DEAE-Sepharose CL-6B chromatographies. By these steps, twelve isozymes of peroxidase were obtained. Some properties of the isozymes were studied and compared.
authors: Department of Agricultural Chemistry, National Taiwan University, Taipei, R.O.C.
authors: H Y
authors: HY
authors: Sung
authors: R H
authors: RH
authors: Yu
authors: C T
authors: CT
authors: Chang
mesh_terms: D000645:Ammonium Sulfate / Q000378:metabolism; D005638:Fruit / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D007527:Isoenzymes / Q000302:isolation & purification*; D007700:Kinetics; D010544:Peroxidases / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D007527:Isoenzymes; D010544:Peroxidases; D000645:Ammonium Sulfate