Automated spectrophotometric method for determining oxidized and reduced glutathione in liver.
An enzymatic recycling method has been applied to the measurement of total and oxidized glutathione with a centrifugal analyzer. When the reduced form of glutathione (GSH) was masked with 2-vinylpyridine to measure the oxidized glutathione (GSSG), the time to ensure full derivatization was three times longer than has been reported. The method is quick, simple, accurate, and precise (1.27% for GSH, 3.3% for GSSG intraassay CV; 2.15% for GSH, 5% for GSSG interassay CV), and the automation allows large numbers of samples to be conveniently assayed.
Teare
J P
JP
Gastrointestional Laboratory, Rayne Institute, London, UK.
Punchard
N A
NA
Powell
J J
JJ
Lumb
P J
PJ
Mitchell
W D
WD
Thompson
R P
RP
D001322:Autoanalysis / Q000706:statistics & numerical data; D005978:Glutathione / Q000032:analysis*; D006801:Humans; D008099:Liver / Q000737:chemistry*; D010084:Oxidation-Reduction; D011786:Quality Control; D013053:Spectrophotometry
D005978:Glutathione