Determination of serum cholesterol concentration in the presence of ascorbate.
Pretreatment of a serum or plasma sample with ascorbate oxidase removed interfering ascorbate and allowed the determination of cholesterol to be carried out by a current enzymatic cholesterol method available in kit form. The Cobas-Fara was programmed to carry out pretreatment of the sample with ascorbate oxidase before addition of the cholesterol colour reagent.
Lumb
P J
PJ
Department of Endocrinology & Chemical Pathology, St Thomas's Hospital, London.
Slavin
B M
BM
D001204:Ascorbate Oxidase; D001205:Ascorbic Acid / Q000097:blood*; D002784:Cholesterol / Q000097:blood*; D006801:Humans; D011933:Reagent Kits, Diagnostic
D011933:Reagent Kits, Diagnostic; D002784:Cholesterol; D001204:Ascorbate Oxidase; D001205:Ascorbic Acid