High-resolution chromosomal localization of the human calcitonin/CGRP/IAPP gene family members.
We report the high-resolution localization of the human calcitonin/CGRP genes, CALCA, CALCB, and the pseudogene CALCP, to a 220-kb SacII fragment on chromosome 11p15.2-p15.1, using prometaphase fluorescence in situ hybridization (FISH), two-color interphase FISH, and pulsed-field gel electrophoresis analysis. The related islet amyloid polypeptide (IAPP) gene was assigned to human chromosome 12p12.3-p12.1. The results support an evolutionary relationship between the calcitonin/CGRP genes and the IAPP gene and between parts of human chromosomes 11 and 12.
Hoovers
J M
JM
Institute of Human Genetics, University of Amsterdam, The Netherlands.
Redeker
E
E
Speleman
F
F
Höppener
J W
JW
Bhola
S
S
Bliek
J
J
van Roy
N
N
Leschot
N J
NJ
Westerveld
A
A
Mannens
M
M
D000682:Amyloid / Q000235:genetics*; D002116:Calcitonin / Q000235:genetics*; D015740:Calcitonin Gene-Related Peptide / Q000235:genetics*; D002874:Chromosome Mapping; D002880:Chromosomes, Human, Pair 11; D002881:Chromosomes, Human, Pair 12; D016521:Electrophoresis, Gel, Pulsed-Field; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D058228:Islet Amyloid Polypeptide; D005810:Multigene Family; D014407:Tumor Cells, Cultured
D000682:Amyloid; D058228:Islet Amyloid Polypeptide; D002116:Calcitonin; D015740:Calcitonin Gene-Related Peptide