title: Heterogeneity in early and advanced gastric carcinomas by flow cytometric DNA analysis.
abstract: We investigated 230 systematically sampled fresh specimens from 12 early and 26 advanced gastric cancer patients by DNA flow cytometry for heterogeneity in DNA content. Fifty-eight percent of the 12 early gastric cancers were uniformly diploid and 42% were uniformly aneuploid. Fifty-four percent of advanced cancers were uniformly diploid in superficial layers and 42% were uniformly diploid in deep layers, whereas 46% were uniformly aneuploid in superficial layers, and 50% were uniformly aneuploid and 8% were heterogeneously aneuploid and diploid in deep layers. Both diploid and aneuploid samples were obtained from 15% for advanced cancers, but ploidy heterogeneity did not occur in early cancers. Heterogeneity for DNA index (more than one aneuploid DNA index) occurred in 46% of whole thickness of advanced cancers, in 19% of superficial layers of advanced cancers, and in 8% of early cancers. We concluded that DNA ploidy determination using superficial layer specimens may be reliable in early gastric cancer but must be interpreted with care in advanced cancer.
authors: First Department of Surgery, Hamamatsu University School of Medicine, Japan.
authors: Y
authors: Y
authors: Umehara
authors: T
authors: T
authors: Kimura
authors: M
authors: M
authors: Yoshida
authors: N
authors: N
authors: Oba
authors: Y
authors: Y
authors: Harada
mesh_terms: D000782:Aneuploidy; D002277:Carcinoma / Q000453:epidemiology / Q000235:genetics*; D004273:DNA, Neoplasm / Q000032:analysis*; D004171:Diploidy; D005260:Female; D005434:Flow Cytometry; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D013274:Stomach Neoplasms / Q000453:epidemiology / Q000235:genetics*
chemical_list: D004273:DNA, Neoplasm