title: Mutant human cells defective in induction of major histocompatibility complex class II genes by interferon gamma.
abstract: Using immunoselection, we have isolated 11 independent mutant HT1080 fibrosarcoma cell lines defective in the induction by interferon gamma (IFN-gamma) of the expression of the human leukocyte antigen HLA-DRA. The mutations are recessive and fall into five complementation groups. All the mutants are affected mainly in the expression of major histocompatibility complex class II and invariant-chain genes. Type I mutants (three complementation groups) are completely defective in induction of the invariant-chain and class II HLA-DP, -DQ, -DR, and -DM genes, whereas type II mutants (two complementation groups) induce these genes weakly in response to IFN-gamma, in the order DPB > DRA > invariant chain. The induction by IFN-gamma of the mRNAs for class I, TAP1, LMP7, and 9-27 is partially defective and the induction of the proteins IRF-1 and ICAM-1 is normal in both types of mutants. All the mutants respond normally to IFN-alpha. The mutants are stable and thus can be used to clone the affected genes by reversion.
authors: Imperial Cancer Research Fund, London, United Kingdom.
authors: C
authors: C
authors: Mao
authors: D
authors: D
authors: Davies
authors: I M
authors: IM
authors: Kerr
authors: G R
authors: GR
authors: Stark
mesh_terms: D000911:Antibodies, Monoclonal; D001483:Base Sequence; D015152:Blotting, Northern; D002459:Cell Fusion; D005354:Fibrosarcoma; D005434:Flow Cytometry; D005802:Genes, MHC Class II / Q000187:drug effects*; D005808:Genes, Recessive; D005816:Genetic Complementation Test; D005819:Genetic Markers; D006681:HLA-D Antigens / Q000032:analysis / Q000235:genetics*; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology*; D008969:Molecular Sequence Data; D016296:Mutagenesis; D009838:Oligodeoxyribonucleotides; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000096:biosynthesis; D011994:Recombinant Proteins; D014407:Tumor Cells, Cultured
chemical_list: D000911:Antibodies, Monoclonal; D005819:Genetic Markers; D006681:HLA-D Antigens; D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger; D011994:Recombinant Proteins; D007371:Interferon-gamma