A rapid molecular diagnosis of posttransfusion graft-versus-host disease by polymerase chain reaction.
A woman with recurrent Paget's disease of the vulva developed acute graft-versus-host disease (GVHD) 12 days after radical surgery and massive blood transfusion. Molecular diagnosis of lymphocyte chimerism in the peripheral blood was made by polymerase chain reaction (PCR) directed against a Y chromosome-specific sex-determining region Y (SRY) gene. PCR with skin biopsy after onset of GVHD also revealed infiltration of SRY-positive donor lymphocytes. The diagnosis was confirmed by HLA-DNA typing with PCR-sequence-specific oligonucleotide that revealed the presence of complex HLA-DR chimerism in the peripheral lymphocytes collected after onset of GVHD. The use of SRY-directed PCR is a rapid technique for the early diagnosis of acute posttransfusion GVHD in female patients.
Hayakawa
S
S
Department of Obstetrics and Gynecology, Nihon University School of Medicine, Tokyo, Japan.
Chishima
F
F
Sakata
H
H
Fujii
K
K
Ohtani
K
K
Kurashina
K
K
Hayakawa
J
J
Suzuki
K
K
Nakabayashi
H
H
Esumi
M
M
D001483:Base Sequence; D002678:Chimera; D004247:DNA / Q000032:analysis; D005260:Female; D006086:Graft vs Host Disease / Q000175:diagnosis* / Q000209:etiology; D006680:HLA Antigens / Q000032:analysis / Q000235:genetics; D006650:Histocompatibility Testing; D006801:Humans; D008214:Lymphocytes / Q000276:immunology / Q000502:physiology; D008875:Middle Aged; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012867:Skin / Q000737:chemistry; D065227:Transfusion Reaction; D014998:Y Chromosome
D006680:HLA Antigens; D004247:DNA