Transfer of CD8+ T cells into SCID mice and activation of memory virus-specific cytotoxic T cells.
The requirements for the activation of naive and memory CD8+ cytotoxic T (Tc) cells into effector virus-specific Tc cells after transferring them into SCID mice were investigated. SCID mice reconstituted with splenocytes or purified CD8+ T cells from naive or influenza-immune syngeneic mice and immunized with influenza virus generated effector Tc cells specific for influenza virus-infected target cells in vitro. The kinetics of the response varied between those two populations. The generation of effector Tc cells after transfer of memory CD8+ T cells indicates that there exists no absolute requirement for 'help' in the activation of memory virus-immune T cells. However, under the conditions described here the in vitro immunogenic peptide NPP derived from influenza nucleoprotein is not sufficient to elicit a response in vivo.
Kos
F J
FJ
Division of Cell Biology, John Curtin School of Medical Research, Australian National University, Camberra.
Müllbacher
A
A
D000818:Animals; D016827:CD8 Antigens / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D016513:Mice, SCID; D009975:Orthomyxoviridae / Q000276:immunology*; D010455:Peptides / Q000494:pharmacology; D013601:T-Lymphocytes / Q000187:drug effects / Q000637:transplantation*; D013602:T-Lymphocytes, Cytotoxic / Q000187:drug effects / Q000276:immunology*
D016827:CD8 Antigens; D010455:Peptides