Differentiation of acute promyelocytic leukemia cell induced by 1,25-dihydroxyvitamin D3 and pentoxifylline (Trental).
Human promyelocytic leukemia cell line HL-60 in tissue culture was treated with various concentrations of 1,25-dihydroxyvitamin D3, (D3) pentoxifylline (Px) or in combinations. Differentiation to normal granulocytes or monocytes was evaluated by nitroblue tetrazolium (NBT) reducing capacity and morphologic criteria. (D3) produced monocytic differentiation, but sub-differentiation inducing doses of (Px) had no effect. In previous studies we found higher doses of (Px) to produce granulocytic differentiation in vitro and in vivo.
Srivastava
M
M
State University of New York, Buffalo Graduate School, Roswell Park Division, Department of Experimental Pathology, NY.
Ambrus
J L
JL
D002117:Calcitriol / Q000494:pharmacology* / Q000633:toxicity; D002454:Cell Differentiation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D006801:Humans; D015473:Leukemia, Promyelocytic, Acute / Q000188:drug therapy* / Q000473:pathology*; D010431:Pentoxifylline / Q000494:pharmacology* / Q000633:toxicity; D014407:Tumor Cells, Cultured / Q000187:drug effects
D002117:Calcitriol; D010431:Pentoxifylline