Evidence for a new tumour suppressor locus (DBM) in human B-cell neoplasia telomeric to the retinoblastoma gene.
Roughly 25% of human B-cell chronic lymphocytic leukaemias (CLL) are characterized by a chromosomal lesion involving 13q14. This region contains the retinoblastoma gene (RB1). We have used a variety of techniques to determine whether RB1 or some other locus is the critical region in 11 cases of low grade B-cell malignancy (mainly CLL), all with deletions or translocations involving 13q14. In all cases, except the one with minimal disease, there was deletion or a structural lesion in the region of D13S25, with at least 4 cases showing homozygous disruption. We conclude that D13S25 lies close to a tumour suppressor locus whose inactivation contributes to the initiation or progression of low grade B-cell malignancy. This locus is located at least 530 kilobases telomeric to RB1.
Brown
A G
AG
MRC Human Genetics Unit, Western General Hospital, Edinburgh, Scotland, UK.
Ross
F M
FM
Dunne
E M
EM
Steel
C M
CM
Weir-Thompson
E M
EM
D000483:Alleles; D000818:Animals; D015139:Blotting, Southern; D002882:Chromosomes, Human, Pair 13; D016521:Electrophoresis, Gel, Pulsed-Field; D016161:Genes, Retinoblastoma; D016147:Genes, Tumor Suppressor; D006801:Humans; D006822:Hybrid Cells; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000235:genetics*; D051379:Mice; D016615:Telomere; D014407:Tumor Cells, Cultured