title: [In vivo and in vitro study of plasmid fragments from Ca2+-dependent Yersinia pestis (Lehmann, Neumann)].
abstract: The bank of the HindIII, EcoRI and PstI fragments of Yersinia pestis Ca(2+)-dependence plasmid (pCaD) was constructed and used as molecular probes, combined with the restriction analysis to map pCaD. Experiments on laboratory animals showed two subfragments of the fifth HindIII fragment to impart virulence, invasiveness to the plasmidless avirulent strains of Y. pestis, Y. pseudotuberculosis and Escherichia coli, and the protective activity to Y. pestis clones, while the original fifth HindIII fragment possessed no such property.
authors: O T
authors: OT
authors: Mozharov
authors: E P
authors: EP
authors: Savostina
authors: P I
authors: PI
authors: Anisimov
authors: G P
authors: GP
authors: Shvedun
authors: I N
authors: IN
authors: Ezhov
authors: I V
authors: IV
authors: Tuchkov
authors: T I
authors: TI
authors: Shiriaeva
mesh_terms: D002118:Calcium / Q000378:metabolism*; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics / Q000472:pathogenicity; D005798:Genes, Bacterial; D010957:Plasmids; D015183:Restriction Mapping; D014774:Virulence / Q000235:genetics; D015010:Yersinia pestis / Q000235:genetics* / Q000472:pathogenicity; D015011:Yersinia pseudotuberculosis / Q000235:genetics / Q000472:pathogenicity
chemical_list: D002118:Calcium
vernacular_title: Izuchenie in vivo i in vitro fragmentov plazmidy Ca2+-zavisimosti Yersinia pestis (Lehmann, Neumann).