Mycelial elongation and sporulation of two fungi on amended media in light or dark.
Botrytis allii and Collectotrichum dematium are onion pathogens which can infect in the field and cause decay in storage. Some phenolics can hinder development of these fungi, but the effect of cytokinins is not clear. Cytokinins (kinetin or 6-benzyladenine) or phenolics (caffeic or chlorogenic acids) were added to agar at concentrations of 0 to 10(-3) M. Cultures were continuously irradiated with fluorescent light or maintained in the dark for 6 days. On unamended media, final mycelial elongation was 45 or 17.8 mm and sporulation was 28 or 10.6 x 10(4) spores/ml for Botrytis and Colletotrichum, respectively. For Botrytis, mycelial elongation was slightly (5%) but significantly increased and sporulation increased by 21% by incubation on phenolics as compared to cytokinins. Mycelial extension of Colletotrichum was not affected by amendment. Sporulation of Colletotrichum on kinetin was 16 to 28% greater than on the other amendments. As amendments concentration increased elongation of mycelia of both fungi decreased. Sporulation of Botrytis increased by 60% as amendment concentration increased from 0 to 10(-5) M and then decreased 25% at 10(-3) M. As amendment concentration increased from 0 to 10(-3) M, sporulation of Colletotrichum increased by 45%. Incubation in light increased mycelial extension 3 to 17% for Botrytis and Colletotrichum respectively, and sporulation was increased approximately 78% for both fungi. These compounds do not appear to inhibit development of these Botrytis or Colletotrichum species in culture.
Russo
V M
VM
United States Department of Agriculture, South Central Agricultural Research Laboratory, Lane, OK 74555.
Pappelis
A J
AJ
D003470:Culture Media; D003583:Cytokinins / Q000494:pharmacology; D003624:Darkness; D008027:Light; D003904:Mitosporic Fungi / Q000187:drug effects / Q000254:growth & development* / Q000502:physiology; D010636:Phenols / Q000494:pharmacology; D010944:Plants / Q000382:microbiology; D013045:Species Specificity; D013172:Spores, Fungal / Q000502:physiology
D003470:Culture Media; D003583:Cytokinins; D010636:Phenols