Cellular sensitivity to native type IV collagen in primary biliary cirrhosis.
We examined 60 patients with various chronic liver diseases for cellular sensitivity to native human type I and IV collagens using an in vitro leucocyte migration inhibition test. Mononuclear cells from 7 (33%) of 21 patients with chronic active hepatitis, 14 (52%) of 27 patients with liver cirrhosis and 11 (92%) of 12 patients with primary biliary cirrhosis exhibited cellular sensitivity to type IV collagen, although cells from almost all patients responded to type I collagen. None except one for type I collagen of 25 normal controls showed positive response to both collagens. In chronic active hepatitis and liver cirrhosis, cellular sensitivity to type IV collagen was significantly lower than to type I collagen (p < 0.01). Patients with primary biliary cirrhosis showed significantly higher cellular sensitivity to type IV collagen when compared to patients with other chronic liver diseases (p < 0.01). Cellular sensitivity to type IV collagen was significantly correlated with serum levels of the 7S domain of type IV collagen in all 85 subjects (r = +0.462, p < 0.001). These findings suggest that cellular sensitivity to type IV collagen as well as to type I collagen exists in chronic liver disease, especially in primary biliary cirrhosis, and may reflect the accelerated metabolism of the perisinusoidal and peribiliary basement membranes.
Suou
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Second Department of Internal Medicine, Tottori University Faculty of Medicine, Yonago, Japan.
Ago
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Sawada
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Hirayama
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D000328:Adult; D000368:Aged; D002464:Cell Migration Inhibition; D003094:Collagen / Q000494:pharmacology*; D005260:Female; D006521:Hepatitis, Chronic / Q000097:blood / Q000188:drug therapy; D006801:Humans; D007961:Leukocyte Migration-Inhibitory Factors / Q000494:pharmacology; D008103:Liver Cirrhosis / Q000097:blood / Q000188:drug therapy; D008105:Liver Cirrhosis, Biliary / Q000097:blood / Q000188:drug therapy* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D012680:Sensitivity and Specificity
D007961:Leukocyte Migration-Inhibitory Factors; D003094:Collagen