title: Enhancement of intracellular uncoating of adenovirus in HeLa cells in the presence of benzyl alcohol as a membrane fluidizer.
abstract: The early extra- and intra-cellular interaction between adenovirus type 2 and HeLa cells was studied in the presence of benzyl alcohol as a fluidizing agent. The process of virus attachment and internalization were not affected at 5-15 mM of benzyl alcohol at 25 degrees C. Under the same conditions an enhancement by 45% at the most was demonstrated for the cell-mediated virion uncoating. By completely blocking virion internalization with 50 mM azide the uncoating was reduced to 20% of the normal level. The remaining surface-located uncoating was not affected by benzyl alcohol. It was demonstrated that an enhancement of the intracellular virion uncoating was followed by a raised production of the hexon antigen, which was interpreted as an increase in the specific infectivity of the virus.
authors: Department of Microbiology, University of Lund, Sweden.
authors: Y
authors: Y
authors: Blixt
authors: M J
authors: MJ
authors: Varga
authors: E
authors: E
authors: Everitt
mesh_terms: D000256:Adenoviridae / Q000502:physiology*; D000956:Antigens, Viral / Q000096:biosynthesis; D019905:Benzyl Alcohol; D001592:Benzyl Alcohols / Q000494:pharmacology*; D002448:Cell Adhesion; D002462:Cell Membrane / Q000187:drug effects / Q000382:microbiology*; D004705:Endocytosis; D006367:HeLa Cells; D006801:Humans; D008560:Membrane Fluidity / Q000187:drug effects; D014168:Transferrin / Q000493:pharmacokinetics
chemical_list: D000956:Antigens, Viral; D001592:Benzyl Alcohols; D014168:Transferrin; D019905:Benzyl Alcohol