title: Non-specific hole formation in the Escherichia coli inner membrane by lambda S proteins in independent of cellular secY and secA functions and of the proportion of membrane acidic phospholipids.
abstract: Formation of the lesion in the Escherichia coli inner membrane caused by lambda lysis protein S was examined by electron microscopy. We also show that macromolecules exceeding the size of the lambda R transglycosylase can pass through the S-dependent hole and that assembly of the S-dependent hole is independent of the proportion of acidic phospholipids in the inner membrane and of components of the cellular transport machinery.
authors: Institute of Microbiology and Genetics, University of Vienna, Austria.
authors: A
authors: A
authors: Rietsch
authors: U
authors: U
authors: Bläsi
mesh_terms: D000251:Adenosine Triphosphatases / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D010582:Bacteriophage lambda / Q000235:genetics / Q000502:physiology*; D001692:Biological Transport; D002462:Cell Membrane; D004926:Escherichia coli / Q000737:chemistry / Q000235:genetics*; D029968:Escherichia coli Proteins; D016695:Glycosyltransferases; D008563:Membrane Lipids / Q000032:analysis*; D026901:Membrane Transport Proteins; D010743:Phospholipids / Q000032:analysis*; D000069816:SEC Translocation Channels; D000081416:SecA Proteins; D013104:Spheroplasts / Q000378:metabolism; D014166:Transferases / Q000378:metabolism; D014764:Viral Proteins / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D008563:Membrane Lipids; D026901:Membrane Transport Proteins; D010743:Phospholipids; D000069816:SEC Translocation Channels; C057841:SecY protein, E coli; D014764:Viral Proteins; D014166:Transferases; D016695:Glycosyltransferases; C021815:murein transglycosylase; D000251:Adenosine Triphosphatases; D000081416:SecA Proteins