Effects of seven months' exposure to a static 0.2 T magnetic field on growth and glycolytic activity of human gingival fibroblasts.
Human gingival fibroblasts in confluent cultures were continuously exposed to a static 0.2 T magnetic field for 6 or 8 months. Culture flasks were not changed during the exposure, but culture medium was renewed. After dilution and mixing of the cultures surviving intact, field-exposed and sham-exposed cultures received further field- or sham-exposure on Sm-Co blocks. Rate of cell proliferation, histogram of the nuclear DNA content, rates of lactate production and glucose consumption and the ATP content were determined and cell morphology was investigated by both light- and electron-microscopy. Results show no marked differences between exposed and control cells.
Yamaguchi
H
H
Department of Physiology, School of Medicine, University of Tokushima, Japan.
Hosokawa
K
K
Soda
A
A
Miyamoto
H
H
Kinouchi
Y
Y
D000255:Adenosine Triphosphate / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D002478:Cells, Cultured; D004247:DNA / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology; D005881:Gingiva / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure; D006019:Glycolysis; D006801:Humans; D008280:Magnetics; D008854:Microscopy, Electron
D000255:Adenosine Triphosphate; D004247:DNA