title: Humeral skeletal development and plasma constituent changes in fetuses of ewes maintained on a low calcium diet from 60 days of gestation.
abstract: The objective of this study was to evaluate the effects of a long-term, low-calcium diet on fetal calcium metabolism and fetal skeleton development in ewes. Eleven pregnant sheep were assigned to two groups, fed either a diet low in calcium (0.26% total dry matter) or normal in calcium (0.8% total dry matter) for 2 months, starting at 60 days gestational age. The ewes fed the low calcium diet showed lower plasma levels of calcium and higher plasma levels of hydroxyproline, parathyroid hormone, and 1,25(OH)2D compared with the ewes fed the normal calcium diet. There were no differences in these variables between the two groups of fetuses. These observations suggest that the plasma components of calcium homeostasis measured in the fetal lamb in the present study are independent of the ewe and are not significantly affected by the presence of lowered maternal calcium for many weeks during pregnancy. Despite the ability of the fetus of the ewe on the low calcium diet to maintain relatively normal circulating plasma components of calcium homeostasis, long-term maternal hypocalcemia delayed fetal skeletal ossification as shown by histological examination of the fetal humerus. The fetal humerus from low calcium-fed ewes showed a lower proportion of bone versus cartilage (45.6 +/- 5.9 versus 57.4 +/- 4.6%, mean +/- SD) lower ash content (15.4 +/- 1.5 versus 17.4 +/- 1.0%), and lower specific gravity (1.19 +/- 0.2 versus 1.22 +/- 0.02) (P < 0.05) than the humerus from fetuses of normal calcium-fed ewes. This study shows that the long-term calcium intake of the ewe does affect fetal skeletal development, despite a lack of observable effects on fetal plasma concentrations of calcium or known calcium regulating hormones such as 1,25(OH)2D or parathyroid hormone.
authors: Department of Pathology, College of Veterinary Medicine, Cornell University, Ithaca, New York 14853.
authors: M S
authors: MS
authors: Lima
authors: F
authors: F
authors: Kallfelz
authors: L
authors: L
authors: Krook
authors: P W
authors: PW
authors: Nathanielsz
mesh_terms: D000818:Animals; D015519:Bone Density; D001842:Bone and Bones / Q000196:embryology*; D002117:Calcitriol / Q000097:blood; D002118:Calcium / Q000097:blood*; D002136:Calcium, Dietary / Q000008:administration & dosage*; D005314:Embryonic and Fetal Development; D004838:Epiphyses / Q000166:cytology / Q000196:embryology; D005260:Female; D005312:Fetal Blood / Q000737:chemistry*; D005333:Fetus / Q000378:metabolism; D005755:Gastrins / Q000097:blood; D006909:Hydroxyproline / Q000097:blood; D010280:Parathyroid Glands / Q000166:cytology / Q000196:embryology; D010281:Parathyroid Hormone / Q000097:blood; D011247:Pregnancy; D012756:Sheep
chemical_list: D002136:Calcium, Dietary; D005755:Gastrins; D010281:Parathyroid Hormone; D002117:Calcitriol; D006909:Hydroxyproline; D002118:Calcium