PS2 integrin requirements in Drosophila embryo and wing morphogenesis.
The Drosophila inflated (if) gene encodes the alpha PS2 subunit of the PS integrins. We describe the generation of new if mutations, their lethal embryonic phenotype, and experiments that examine the spatial and temporal requirements for integrins in adult wing morphogenesis. Embryos hemizygous for either new allele, ifA7 or ifB2, make reduced amounts of alpha PS2. In a variety of genetic tests, these alleles behave similarly to ifk27e, which makes no detectable alpha PS2, and all three alleles display the same embryonic phenotype. We therefore conclude that all of the lethal alleles retain little or no wild-type alpha PS2 function. As seen for strong mutations at the myospheroid (mys) locus, which encodes the beta PS integrin subunit, if mutants show extreme defects in somatic muscle attachments and in midgut morphogenesis. Unlike mys, however, there is no dorsal herniation of the if mutant embryos. With respect to wing morphogenesis, clonal analysis experiments demonstrate that if+ function is required only in cells of the ventral wing surface. We have rescued the wing blister phenotype of double mutants for the hypomorphic mysnj42 and if3 alleles using a heat shock-inducible mys+ transgene. By varying times of transgene induction, we find that integrin function is required from very early in metamorphosis until at least the last 24-48 hr of wing development.
Brabant
M C
MC
Department of Biochemistry, University of Arizona, Tucson 85721.
Brower
D L
DL
D000483:Alleles; D000818:Animals; D004330:Drosophila / Q000196:embryology* / Q000235:genetics; D029721:Drosophila Proteins; D004625:Embryo, Nonmammalian / Q000502:physiology; D005786:Gene Expression Regulation; D005804:Genes, Lethal; D039001:Integrin alpha Chains; D016023:Integrins / Q000235:genetics*; D007814:Larva; D009024:Morphogenesis; D010641:Phenotype; D014921:Wings, Animal / Q000166:cytology / Q000196:embryology
D029721:Drosophila Proteins; D039001:Integrin alpha Chains; D016023:Integrins; C082188:if protein, Drosophila