Involvement of superoxide dismutase and glutathione peroxidase in attenuation of radiation-induced hyperthermia by interleukin-1 alpha in rats.
Pretreatment with recombinant human interleukin-1 alpha (rhIL-1 alpha) 20 h before irradiation attenuates radiation-induced hyperthermia. Experiments were conducted to determine the role of antioxidant enzymes such as superoxide dismutase (SOD) and glutathione peroxidase (GSHPx) in rhIL-1 alpha-induced attenuation of radiation-induced hyperthermia. Radiation exposure increased SOD and decreased GSHPx levels in the hypothalamus, while treatment with rhIL-1 alpha increased GSHPx levels and had no effect on SOD levels. However, rhIL-1 alpha and irradiation together increased hypothalamic SOD level but prevented the fall in GSHPx level. Our results suggest that attenuation of radiation-induced hyperthermia by rhIL-1 alpha may involve stimulation of SOD and GSHPx because rhIL-1 alpha treatment and irradiation together increased hypothalamic GSHPx and SOD levels, and intracerebroventricular administration of SOD and GSHPx attenuated the radiation-induced hyperthermia.
Kandasamy
S B
SB
Behavioral Sciences Department, Armed Forces Radiobiology Research Institute, Bethesda, MD 20889-5145.
Kumar
K S
KS
Harris
A H
AH
D000818:Animals; D005334:Fever / Q000628:therapy*; D005979:Glutathione Peroxidase / Q000378:metabolism*; D007031:Hypothalamus / Q000201:enzymology; D007276:Injections, Intraventricular; D007375:Interleukin-1 / Q000627:therapeutic use*; D008297:Male; D011833:Radiation Injuries, Experimental / Q000628:therapy*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins; D012643:Selenium / Q000494:pharmacology; D013482:Superoxide Dismutase / Q000378:metabolism*
D007375:Interleukin-1; D011994:Recombinant Proteins; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D012643:Selenium