Comparison of the Seradyn Color Vue passive agglutination test and complement fixation for detection of Mycoplasma pneumoniae antibodies.
We compared traditional complement fixation (CF) with a new passive agglutination method, the Seradyn Color Vue (SCV) test (Seradyn, Indianapolis, Ind.), for detection of Mycoplasma pneumoniae antibodies in 170 stored serum samples. The SCV test was 90% sensitive in identifying as positive 27 of 30 CF high-titer (> or = 1:64) serum samples and 100% specific in identifying as negative 134 of 134 CF low-titer (< or = 1:32) or negative (< 1:8) serum samples. The SCV test was technically undemanding, and it required no expensive equipment.
Leland
D S
DS
Department of Pathology, Indiana University Medical Center, Indianapolis 46202-5200.
Barth
K A
KA
Cunningham
E B
EB
D000372:Agglutination Tests; D000907:Antibodies, Bacterial / Q000097:blood*; D003168:Complement Fixation Tests; D006801:Humans; D009177:Mycoplasma pneumoniae / Q000276:immunology*; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity
D000907:Antibodies, Bacterial; D011933:Reagent Kits, Diagnostic