Detection of DNA in Southern blots with chemiluminescence.
The chemiluminescent detection methods described in this chapter have been successfully applied to the detection of plasmid DNA and genomic DNA in Southern and sequencing protocols. The high sensitivity and the simplicity of AMPPD are instrumental in making the chemiluminescent detection of DNA successful in hybridization assays. This detection technique has also been used to detect DNA in dot blots and in situ hybridization experiments as well as proteins in enzyme-linked immunosorbent assays (ELISAs) and Western blots.
Bronstein
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Tropix, Inc., Bedford, Massachusetts 01730.
Voyta
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Murphy
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Tizard
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Ehrenfels
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D000218:Adamantane / Q000031:analogs & derivatives; D000469:Alkaline Phosphatase; D001345:Autoradiography / Q000379:methods; D001483:Base Sequence; D015139:Blotting, Southern / Q000379:methods; D004247:DNA / Q000032:analysis* / Q000235:genetics; D004274:DNA, Recombinant / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D017403:In Situ Hybridization; D007202:Indicators and Reagents; D008163:Luminescent Measurements; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes; D010761:Phosphorus Radioisotopes; D010957:Plasmids; D011506:Proteins / Q000032:analysis
D004274:DNA, Recombinant; D007202:Indicators and Reagents; D015345:Oligonucleotide Probes; D010761:Phosphorus Radioisotopes; D011506:Proteins; C061523:3-(2'-spiroadamantane)-4-methoxy-4-(3''-phosphoryloxy)phenyl-1,2-dioxetane; D004247:DNA; D000469:Alkaline Phosphatase; D000218:Adamantane