Alternate interactions of the D-galactose-specific yeast lectin Kb-CWL I with sensitive yeast strains.
In vitro growth assays with a purified D-galactose-specific lectin Kb-CWL I extracted from Kluyveromyces bulgaricus showed marked antifungal effects on 9 of 12 test strains belonging to the genera Kluyveromyces, Saccharomyces, Pichia, Candida, Rhodotorula and Schizosaccharomyces. The inhibition of growth was proportional to the lectin concentration (0.04 and 0.08 mg ml-1) in the culture medium. Under the test conditions, K. bulgaricus, K. lactis and S. bayanus were consistently agglutinated without growth inhibition. D-Galactose, the inhibitory sugar of the cell aggregation activity, did not abolish the antifungal effects of the lectin Kb-CWL I on other yeast strains tested. In C. albicans and C. tropicalis, the lectin influenced the dimorphism of these strains and stimulated germ tube formation.
Viard
B
B
Laboratoire de Biochimie Microbienne, Faculté de Pharmacie, Université de Nancy I, France.
al Mahmood
S
S
Streiblova
E
E
Bonaly
R
R
D000935:Antifungal Agents / Q000378:metabolism / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D010450:Endopeptidases / Q000378:metabolism; D005427:Flocculation; D005656:Fungal Proteins / Q000378:metabolism; D005690:Galactose / Q000378:metabolism* / Q000494:pharmacology; D007716:Kluyveromyces / Q000737:chemistry*; D037102:Lectins / Q000378:metabolism / Q000494:pharmacology*; D008358:Mannose / Q000494:pharmacology; D009024:Morphogenesis / Q000187:drug effects; D013045:Species Specificity; D015003:Yeasts / Q000187:drug effects / Q000254:growth & development
D000935:Antifungal Agents; D005656:Fungal Proteins; D037102:Lectins; D010450:Endopeptidases; D008358:Mannose; D005690:Galactose