Lipoprotein and apoprotein profile of Japanese quail.
The present study delineated the lipoprotein and apoprotein distribution in Japanese quail. Quail lipoprotein was composed of three fractions: VLDL, d < 1.020; LDL, 1.020 < d < 1.081; HDL, 1.081 < d < 1.210. When animals were fed the cholesterol-free diet, HDL was the predominant form, LDL intermediate, VLDL and chylomicron were smallest in amount. Feeding of cholesterol induced a marked change in the lipoprotein profile: VLDL or chylomicron predominated over HDL and LDL. An apoprotein of 26 kD (molecular weight) was the major protein moiety comprising more than 50% of total apoprotein in the entire density range of lipoprotein class. Amino acid composition of 26 kD protein was similar to hen, rat and human apo A-I. N-Terminal 36 amino acid sequence of 26 kD protein showed 92% homology to chicken apo A-I and 11% homology to human apo A-1. The 26 kD protein did not react with the antibody raised against human apo A-I. These observation showed that the 26 kD protein was partially identical to apo A-I.
Oku
H
H
Laboratory of Applied Biochemistry, Faculty of Agriculture, University of The Ryukyus, Okinawa, Japan.
Ishikawa
M
M
Nagata
J
J
Toda
T
T
Chinen
I
I
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001053:Apolipoproteins / Q000032:analysis*; D001161:Arteriosclerosis / Q000378:metabolism; D002791:Cholesterol, Dietary / Q000378:metabolism; D003370:Coturnix / Q000378:metabolism*; D008074:Lipoproteins / Q000032:analysis* / Q000302:isolation & purification; D008297:Male; D008969:Molecular Sequence Data; D008970:Molecular Weight; D051381:Rats; D017208:Rats, Wistar
D000596:Amino Acids; D001053:Apolipoproteins; D002791:Cholesterol, Dietary; D008074:Lipoproteins