title: A Ca(2+)-independent protein kinase C from fission yeast.
abstract: A protein kinase C homologue of Schizosaccharomyces pombe, pkc1+, was isolated from a genomic library by screening with the Saccharomyces cerevisiae PKC1 probe. From its primary sequence and biochemical properties, we conclude that S. pombe pkc1+ encodes a phospholipid-activated Ca(2+)-independent protein kinase, homologous to the delta/epsilon classes of mammalian protein kinase C. Gene disruption experiments show that pkc1+ is not essential for cell viability; however, overexpression of the protein leads to an abnormal cell morphology and a block in cell separation following mitosis suggestive of a role in cell division. In vitro phosphorylation experiments reveal several potential pkc1+ substrates.
authors: Glaxo Institute for Molecular Biology, Geneva, Switzerland.
authors: G J
authors: GJ
authors: Mazzei
authors: E M
authors: EM
authors: Schmid
authors: J K
authors: JK
authors: Knowles
authors: M A
authors: MA
authors: Payton
authors: K G
authors: KG
authors: Maundrell
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D002118:Calcium / Q000378:metabolism*; D004271:DNA, Fungal; D008969:Molecular Sequence Data; D010641:Phenotype; D011493:Protein Kinase C / Q000235:genetics / Q000378:metabolism*; D015183:Restriction Mapping; D012568:Schizosaccharomyces / Q000201:enzymology* / Q000235:genetics
chemical_list: D004271:DNA, Fungal; D011493:Protein Kinase C; D002118:Calcium