Human insulin receptor substrate-1 gene (IRS1): chromosomal localization to 2q35-q36.1 and identification of a simple tandem repeat DNA polymorphism.
The protein designated as insulin receptor substrate-1 (IRS-1) is a major substrate for the insulin receptor tyrosine kinase. Since post-receptor defects in the insulin signalling pathway are a common feature of Type 2 (non-insulin-dependent) diabetes mellitus, we have cloned the human IRS-1 gene in order to study the role of genetic variation in this gene in the pathogenesis of diabetes mellitus. As a first step in these studies, we have mapped the IRS-1 gene to chromosome 2, bands q35-q36.1 and identified a simple tandem repeat DNA polymorphism in this gene that will be useful for genetic studies.
Stoffel
M
M
Howard Hughes Medical Institute, Department of Biochemistry, Chicago, Illinois.
Espinosa
R
R
Keller
S R
SR
Lienhard
G E
GE
Le Beau
M M
MM
Bell
G I
GI
D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D002889:Chromosomes, Human, Pair 2; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D015698:Genomic Library; D006801:Humans; D006822:Hybrid Cells; D017403:In Situ Hybridization; D055504:Insulin Receptor Substrate Proteins; D007621:Karyotyping; D008214:Lymphocytes / Q000502:physiology; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D010750:Phosphoproteins / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D012091:Repetitive Sequences, Nucleic Acid
C526292:IRS1 protein, human; D055504:Insulin Receptor Substrate Proteins; C526295:Irs1 protein, mouse; D009838:Oligodeoxyribonucleotides; D010750:Phosphoproteins; D004247:DNA