Antinociceptive activity of salmon calcitonin: electrophysiological correlates in a rat chronic pain model.
Experimental and clinical evidence testifies to an antinociceptive action of salmon calcitonin (sCT), administered in different ways, on the central nervous system. These studies were performed almost exclusively in acute pain models. The purpose of the present study was to investigate the effects of sCT, injected directly into the lateral cerebral ventriculi, on the firing of single nociceptive thalamic neurons, detected by electrophysiological techniques in an experimental model of prolonged or chronic pain, such as rats rendered arthritic by injection of Freund's adjuvant into the left hindfoot. The noxious test stimuli used were either extension or flexion of the ankle or mild lateral pressure on the heel. With increasing doses of sCT (5, 10, 20, 40 micrograms, 5 microliters/i.c.v.) it was possible to observe correspondingly increasing inhibitory and long-lasting effects on the evoked firing, with a significant dose-effect relationship. In agreement with electrophysiological findings, preliminary data, obtained with a patch clamp technique, on depression of calcium fluxes through neuronal membrane, induced by sCT, oriented the attention to a direct action of sCT on CNS.
Braga
P C
PC
Department of Pharmacology, School of Medicine, University of Milan, Italy.
Dal Sasso
M
M
Bernini
A
A
Bartucci
F
F
Pollo
A
A
Carbone
E
E
D000700:Analgesics / Q000008:administration & dosage / Q000494:pharmacology*; D000818:Animals; D001169:Arthritis, Experimental / Q000503:physiopathology; D002116:Calcitonin / Q000008:administration & dosage / Q000494:pharmacology*; D002908:Chronic Disease; D004195:Disease Models, Animal; D004594:Electrophysiology; D007276:Injections, Intraventricular; D008297:Male; D009068:Movement / Q000502:physiology; D009619:Nociceptors / Q000187:drug effects; D010146:Pain / Q000503:physiopathology*; D010812:Physical Stimulation; D011312:Pressure; D051381:Rats; D017207:Rats, Sprague-Dawley; D013788:Thalamus / Q000166:cytology / Q000502:physiology
D000700:Analgesics; C028815:salmon calcitonin; D002116:Calcitonin