Preparation of a monoclonal antibody directed against the receptor for murine colony-stimulating factor-1.
Studies were undertaken to produce monoclonal antibodies directed against the murine receptor for macrophage colony-stimulating factor (M-CSF or CSF-1). Sprague-Dawley rats were injected with lysates prepared from a murine myelomonocytic cell line (RAW cell line) that has high levels of M-CSF receptors. Spleen cells from immunized animals were fused with murine plasmacytoma cells and expanded. Supernatants from these cells caused inhibition of 125I-CSF binding to either RAW cells or normal murine marrow cells. Antibody-producing cells were cloned by limiting dilution and by colony growth in agar. The antireceptor antibodies appear specific as they neutralize colony formation by M-CSF but have little or no effect on colony growth in response to the other hemopoietic growth factors granulocyte CSF (G-CSF), granulocyte-macrophage CSF (GM-CSF), interleukin-3 (IL-3) or erythropoietin. These antibodies should aid in defining the role of M-CSF in hemopoiesis in vivo.
Shadduck
R K
RK
Western Pennsylvania Cancer Institute, West Penn Hospital, Pittsburgh 15224.
Waheed
A
A
Mangan
K F
KF
Rosenfeld
C S
CS
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D001667:Binding, Competitive; D002478:Cells, Cultured; D006410:Hematopoiesis; D006801:Humans; D016173:Macrophage Colony-Stimulating Factor / Q000378:metabolism; D051379:Mice; D016186:Receptor, Macrophage Colony-Stimulating Factor / Q000276:immunology*; D013045:Species Specificity
D000911:Antibodies, Monoclonal; D016173:Macrophage Colony-Stimulating Factor; D016186:Receptor, Macrophage Colony-Stimulating Factor