Multiple mRNA species of choline acetyltransferase from rat spinal cord.
A cDNA library directed by a specific primer was constructed from the rat spinal cord and screened with 32P-labeled rat choline acetyltransferase cDNA which was recently isolated in this laboratory. Sequence analysis of 29 clones indicated that there are four types of cDNA (R1-, R2-, N1- and M-types). The nucleotide sequences in these cDNAs were identical in the coding region and the first 38 bp of the 5'-noncoding region, but differed in the 5'-noncoding region upstream of -38 bp. The R1-type was identical to the cDNA previously cloned from the rat spinal cord. The M and N1-type cDNAs both had sequences homologous to that of the cDNA previously obtained from the mouse spinal cord. Polymerase chain reaction analysis confirmed the presence of these 4 types of mRNA and found another type (N2-type) of transcript. The numbers of cDNA clones isolated and the relative amounts of polymerase chain reaction products for each type of mRNA suggested that the most abundant transcript was M-type. Sequencing of the genomic clone containing the 5'-region of choline acetyltransferase mRNA revealed that these five types of mRNA species were transcribed from three different promoter regions and produced by differential splicing of the 5'-noncoding exons.
Kengaku
M
M
Department of Molecular Neurobiology, Tokyo Metropolitan Institute for Neuroscience, Japan.
Misawa
H
H
Deguchi
T
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002454:Cell Differentiation; D002795:Choline O-Acetyltransferase / Q000235:genetics*; D004247:DNA / Q000235:genetics; D005091:Exons; D015971:Gene Expression Regulation, Enzymologic; D009419:Nerve Tissue Proteins / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D012323:RNA Processing, Post-Transcriptional; D012333:RNA, Messenger / Q000235:genetics*; D051381:Rats; D012689:Sequence Homology, Nucleic Acid; D013116:Spinal Cord / Q000201:enzymology*; D014158:Transcription, Genetic
D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D004247:DNA; D002795:Choline O-Acetyltransferase