[Change in adenine nucleotide pool in E. coli 1257 bacterial cells under the action of a low intensity He-Ne laser].
Evidence is presented of co-immobilized bioluminescent reagents which include firefly luciferase, pyruvate kinase and adenylate kinase used for an adenylate assay in bacterial cells. The changes in the ATP, ADP and AMP content induced by irradiation with a low-power He-Ne-laser depend on the laser power and irradiation dose. The data obtained suggest that laser irradiation causes the activation of adenine nucleotide synthesis de novo.
Romanova
N A
NA
Brovko
L Iu
LIu
Sepul'veda-Bessera
M A
MA
Ugarova
N N
NN
D000227:Adenine Nucleotides / Q000378:metabolism*; D000262:Adenylyl Cyclases; D000818:Animals; D001517:Coleoptera / Q000201:enzymology; D004800:Enzymes, Immobilized; D004926:Escherichia coli / Q000254:growth & development / Q000378:metabolism* / Q000528:radiation effects; D006371:Helium; D007834:Lasers; D008156:Luciferases; D009356:Neon; D010100:Oxygen / Q000378:metabolism; D011770:Pyruvate Kinase
D000227:Adenine Nucleotides; D004800:Enzymes, Immobilized; D006371:Helium; D009356:Neon; D008156:Luciferases; D011770:Pyruvate Kinase; D000262:Adenylyl Cyclases; D010100:Oxygen
Izmenenie pula adeninovykh nukleotidov v kletkakh bakterii E. coli 1257 pri vozdeĭstvii nizkointensivnogo He-Ne lazera.