Ethanol exposure increases total protein kinase C activity in human lymphocytes.
Human circulating lymphocytes were isolated and incubated with ethanol. Cytosolic, membrane-bound and total detergent extractable protein kinase C (PKC) activities were measured. Exposure to ethanol (100 mm) resulted in an increase in PKC activity, with membrane-associated PKC activity increasing with respect to cytosolic activity at 5 min of exposure. Higher concentrations of ethanol up to 200 mm were associated with increases in total detergent extractable PKC activity. Ethanol was the most potent of a series of straight chain alcohols studied for their effects on detergent-extractable PKC activity.
DePetrillo
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PB
Division of Clinical Pharmacology, Brown University School of Medicine, Providence, Rhode Island.
Liou
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CS
D020001:1-Butanol; D000433:1-Propanol / Q000494:pharmacology; D000440:Butanols / Q000494:pharmacology; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D000431:Ethanol / Q000494:pharmacology*; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects* / Q000201:enzymology; D000432:Methanol / Q000494:pharmacology; D011493:Protein Kinase C / Q000378:metabolism*
D000440:Butanols; D000431:Ethanol; D020001:1-Butanol; D000433:1-Propanol; D011493:Protein Kinase C; D000432:Methanol