Electrical and mechanical changes during anoxic contractions of the isolated canine basilar artery.
Isometric tension and membrane potential were measured to determine the electrophysiological events occurring during anoxia in the isolated canine basilar artery. Anoxia induced transient contractions which were inhibited by the Ca(2+)-channel inhibitor, diltiazem, and were abolished in Ca(2+)-free solution. Anoxic contractions were accompanied by membrane depolarizations, which were resistant to diltiazem. When matched contractions were obtained with anoxia and high K+, the level of membrane depolarization was smaller during anoxic contractions. These results support the importance of voltage-dependent Ca2+ influx in the generation of anoxic contractions in the canine basilar artery. However, membrane depolarization does not fully account for these anoxic contractions.
Nagao
T
T
Center for Experimental Therapeutics, Baylor College of Medicine, Houston, TX 77030.
Vanhoutte
P M
PM
D000818:Animals; D001488:Basilar Artery / Q000187:drug effects / Q000473:pathology / Q000503:physiopathology*; D002118:Calcium / Q000494:pharmacology; D004110:Diltiazem / Q000494:pharmacology; D004285:Dogs; D004594:Electrophysiology; D005260:Female; D000860:Hypoxia / Q000503:physiopathology*; D066298:In Vitro Techniques; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000473:pathology / Q000503:physiopathology; D011188:Potassium / Q000494:pharmacology; D014661:Vasoconstriction / Q000502:physiology*
D004110:Diltiazem; D011188:Potassium; D002118:Calcium