title: Preferential stimulation by D-glucose of oxidative glycolysis in pancreatic islets: comparison between B and non-B cells.
abstract: In purified pancreatic islet B cells, a rise in D-glucose concentration from 2.8 to 16.7 mM increased the production of both 14CO2 from D-[3,4-14C]glucose and 3HOH from D-[5-3H]glucose to a much greater relative extent than in purified non-B islet cells. Moreover, the paired ratio between D-[3,4-14C]glucose oxidation and D-[5-3H]glucose utilization was significantly increased, as a result of the rise in hexose concentration, in purified B cells, but not so in purified non-B cells. It is proposed, therefore, that a preferential stimulation by D-glucose of oxidative relative to total glycolysis represents an intrinsic attribute of insulin-producing cells, as distinct from other endocrine islet cells.
authors: Laboratory of Experimental Medicine, Brussels Free University, Belgium.
authors: D
authors: D
authors: Mercan
authors: J P
authors: JP
authors: Delville
authors: V
authors: V
authors: Leclercq-Meyer
authors: W J
authors: WJ
authors: Malaisse
mesh_terms: D000818:Animals; D002250:Carbon Radioisotopes; D005260:Female; D005947:Glucose / Q000378:metabolism / Q000494:pharmacology*; D006019:Glycolysis; D007515:Islets of Langerhans / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D010084:Oxidation-Reduction; D051381:Rats; D013268:Stimulation, Chemical; D014316:Tritium
chemical_list: D002250:Carbon Radioisotopes; D014316:Tritium; D005947:Glucose