Comparative study of the properties of plasmid mediated staphylococcal beta-lactamase as expressed in Escherichia coli.
An unmodified natural plasmid of Staphylococcal origin having beta-lactamase determinant was introduced by transformation into Gram-negative Escherichia coli HB101 recA-SmR (plasmidless). Beta-lactamase of Staphylococcus aureus is extracellular. In recipient transformed E. coli HB101, the enzyme shows periplasmic location. The enzyme is expressed well in E. coli, and the basal level of enzyme production in E. coli, is approximately 3-4 fold higher than that of S. aureus. Beta-lactamase is inducible both in S. aureus and E. coli. Enzymatic properties as indicated by substrate profile and Km values are found to be similar.
Saha
D
D
Department of Physiology, University College of Science and Technology, Calcutta, India.
Bal
M
M
D003001:Cloning, Molecular; D003470:Culture Media; D004790:Enzyme Induction; D004926:Escherichia coli; D007700:Kinetics; D010957:Plasmids; D013211:Staphylococcus aureus / Q000201:enzymology*; D014169:Transformation, Bacterial; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism*
D003470:Culture Media; D001618:beta-Lactamases