title: Annexin I interactions with human neutrophil specific granules: fusogenicity and coaggregation with plasma membrane vesicles.
abstract: The interactions of annexin I with specific granules isolated from human neutrophils were investigated. Unfractionated cytosol induced Ca(2+)-dependent granule self-aggregation and fusion of granules with model phospholipid vesicles. High Ca2+ concentrations were required for these processes (500-600 microM for the half-maximal rate of granule self-aggregation; 100-200 microM for the half-maximal rate of fusion with phospholipid vesicles). These activities were inhibited by a monoclonal antibody specific for annexin I and immunodepletion of cytosol by this antibody greatly reduced activity, implicating annexin I as the major mediator of these processes in neutrophil cytosol. The fact that the Ca2+ concentration dependences differed for different membranes suggests that specificity may be controlled by the type of intracellular membrane involved and the local Ca2+ concentration. Trypsin treatment of granules enhanced the rate of fusion of phospholipid vesicles with granules, suggesting that access to phospholipids in the granule membrane may be modulated by granule proteins or that a fusogenic protein factor in the granule membrane is activated by trypsin treatment. Coaggregation of specific granules with plasma membrane vesicles mediated by Ca2+ and annexin I was suggested by the fact that granules preincubated with Ca2+, cytosol and plasma membrane vesicles blocked the fusion of subsequently added phospholipid vesicles with the plasma membrane vesicles. These data suggest a role for annexin I as part of a multiprotein system involved in membrane-membrane contact necessary for exocytosis of specific granules in human neutrophils.
authors: Department of Pathology, Boston University School of Medicine, MA 02118.
authors: P
authors: P
authors: Meers
authors: T
authors: T
authors: Mealy
authors: A I
authors: AI
authors: Tauber
mesh_terms: D017305:Annexin A1 / Q000494:pharmacology*; D002118:Calcium / Q000494:pharmacology; D002462:Cell Membrane / Q000187:drug effects; D003594:Cytoplasmic Granules / Q000187:drug effects*; D003600:Cytosol / Q000187:drug effects; D006801:Humans; D008081:Liposomes / Q000737:chemistry; D046911:Macromolecular Substances; D009504:Neutrophils / Q000187:drug effects* / Q000648:ultrastructure; D010743:Phospholipids / Q000737:chemistry; D014357:Trypsin
chemical_list: D017305:Annexin A1; D008081:Liposomes; D046911:Macromolecular Substances; D010743:Phospholipids; D014357:Trypsin; D002118:Calcium