Construction of an aroA mutant of Salmonella serotype Gallinarum: its effectiveness in immunization against experimental fowl typhoid.
An aroA mutant was produced from a virulent strain of Salmonella Gallinarum, the causative agent of fowl typhoid in poultry, by Tn10 insertional inactivation and deletion. The mutant was highly attenuated for chickens. A single intramuscular immunization of 2-week-old chickens with 10(7) mutant organisms reduced mortality following oral challenge with 10(8) organisms of the parent strain from 63 to 30%. In a second experiment multiple immunizations with 10(8) mutant organisms reduced mortality after challenge with 10(7) organisms of the virulent strain from 77 to 0%. By oral inoculation the mutant was not able to immunize chickens against oral challenge.
Griffin
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HG
AFRC Institute for Animal Health, Houghton Laboratory, Huntingdon, Cambs, UK.
Barrow
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PA
D000818:Animals; D001428:Bacterial Vaccines / Q000302:isolation & purification / Q000494:pharmacology*; D002645:Chickens; D005798:Genes, Bacterial; D007114:Immunization; D009154:Mutation; D012475:Salmonella / Q000145:classification / Q000235:genetics* / Q000276:immunology*; D012481:Salmonella Infections, Animal / Q000517:prevention & control*; D012703:Serotyping; D014613:Vaccines, Attenuated / Q000302:isolation & purification / Q000494:pharmacology
D001428:Bacterial Vaccines; D014613:Vaccines, Attenuated