Purification and properties of a 155 kDa bone marrow-derived glycoprotein enhancing the activity of granulocyte-macrophage colony stimulating factor (GM-CSF).
A protein has been purified from pig bone marrow which enhances the activity of exogenously added granulocyte-macrophage colony stimulating factor (GM-CSF) on mouse myelopoietic colony formation in vitro. The substance alone did not exhibit any colony stimulating activity. The active fraction was isolated from the medium of pig bone marrow suspension cultures during 20-24 h. Purification to homogeneity was then performed by subsequent HPLC and SDS-PAGE. The GM-CSF enhancing protein was identified as a 155 kDa glycoprotein.
Katlinsky
A V
AV
Gade Institute, Department of Pathology, University of Bergen, Haukeland Hospital, Norway.
Petrov
R V
RV
Mikhailova
A A
AA
Laerum
O D
OD
D000818:Animals; D015153:Blotting, Western; D001853:Bone Marrow / Q000737:chemistry* / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D017077:Culture Media, Conditioned; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000302:isolation & purification* / Q000378:metabolism; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000378:metabolism*; D008970:Molecular Weight; D013552:Swine
D017077:Culture Media, Conditioned; D006023:Glycoproteins; D016178:Granulocyte-Macrophage Colony-Stimulating Factor