Effects of guanidine hydrochloride on catalytic efficiency of glycine amidinotransferase of rat.
Guanidine-induced alterations in substrate-dependent kinetics of glycine amidinotransferase (GAT) have been investigated in homogenates of rat kidney. Guanidine hydrochloride (GuHCl) induced a mixed type of inhibition by decreasing the maximal velocity (Vmax) and increasing the Michaelis-Menten constant (Km). The finding of a value of Ki smaller than that of Ki' denoted that the inhibition of GAT may be due to decreased E to S affinity rather than to reduction in the active site density of the enzyme.
Rao
S V
SV
Department of Zoology, Sri Venkateswara University, Tirupati, India.
Indira
K
K
D000579:Amidinotransferases / Q000378:metabolism*; D000818:Animals; D019791:Guanidine; D006146:Guanidines / Q000494:pharmacology*; D007668:Kidney / Q000201:enzymology; D007700:Kinetics; D008297:Male; D051381:Rats; D017208:Rats, Wistar
D006146:Guanidines; D000579:Amidinotransferases; C020681:glycine amidinotransferase; D019791:Guanidine