Human monocyte chemotactic protein-3 (MCP-3): molecular cloning of the cDNA and comparison with other chemokines.
When human MG-63 osteosarcoma cells are triggered with IL-1 beta, they produce, among various other cytokines, also monocyte chemotactic proteins (MCPs). Homogeneous MCP-1, MCP-2 and MCP-3 were found to induce production of gelatinase B and chemotaxis of monocytes. Based on the almost complete amino acid sequence of natural MCP-3, two sets of degenerated oligonucleotides were used for the amplification of MCP-3 cDNA. Total RNA from stimulated MG-63 cells was reverse transcribed and PCRs done on the mRNA:cDNA duplex. Using the PCR-product, several cDNAs were isolated from a cDNA library of IL-1-stimulated MG-63 cells. From the cDNA sequence the complete primary structure of the protein was deduced: MCP-3 shows 71% and 58% amino acid homology with MCP-1 and MCP-2, respectively. Our study establishes MCP-3 as an inflammatory cytokine that regulates macrophage functions. Because MCP-3 is often produced by tumor cell lines and regulates protease secretion by macrophages, its production might also contribute to invasion and metastasis of cancer cells.
Opdenakker
G
G
Rega Institute, Laboratory of Molecular Medicine and Immunobiology, University of Leuven, Belgium.
Froyen
G
G
Fiten
P
P
Proost
P
P
Van Damme
J
J
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018932:Chemokine CCL2; D054410:Chemokine CCL7; D054412:Chemokine CCL8; D002630:Chemotactic Factors / Q000235:genetics*; D002634:Chemotaxis, Leukocyte; D003001:Cloning, Molecular; D017364:Collagenases / Q000378:metabolism; D016207:Cytokines / Q000235:genetics*; D004247:DNA; D006801:Humans; D020780:Matrix Metalloproteinase 9; D008969:Molecular Sequence Data; D018945:Monocyte Chemoattractant Proteins; D009000:Monocytes / Q000166:cytology; D012333:RNA, Messenger / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D014407:Tumor Cells, Cultured
C515450:CCL7 protein, human; C515453:CCL8 protein, human; D018932:Chemokine CCL2; D054410:Chemokine CCL7; D054412:Chemokine CCL8; D002630:Chemotactic Factors; D016207:Cytokines; D018945:Monocyte Chemoattractant Proteins; D012333:RNA, Messenger; D004247:DNA; D017364:Collagenases; D020780:Matrix Metalloproteinase 9