Effect of varying levels of glucose on oxygen deprivation-induced delayed neuronal cell death in primary cerebrocortical cultures.
Calcium accumulation and neuronal injury of rat cortical cell cultures in vitro were examined following oxygen deprivation under conditions of normal and low glucose. 45Ca2+ uptake and lactate dehydrogenase (LDH) release, measured at 12 and 24 h after oxygen deprivation, were significantly elevated in cultures exposed to combined oxygen deprivation and low glucose (1.7 or 0.6 mM). Although those cultures deprived of oxygen combined with no glucose displayed delayed increases in 45Ca2+ influx, no significant elevation in LDH release at 24 h was observed.
Birrell
G J
GJ
Parke-Davis Pharmaceutical Research Division, Warner-Lambert Company, Ann Arbor, MI 48106.
Marcoux
F W
FW
D000818:Animals; D002118:Calcium / Q000378:metabolism; D002132:Calcium Radioisotopes; D016923:Cell Death / Q000502:physiology; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology; D003470:Culture Media; D005260:Female; D005947:Glucose / Q000494:pharmacology*; D002534:Hypoxia, Brain / Q000503:physiopathology*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D009457:Neuroglia / Q000187:drug effects / Q000502:physiology; D009474:Neurons / Q000502:physiology*; D011247:Pregnancy; D051381:Rats
D002132:Calcium Radioisotopes; D003470:Culture Media; D007770:L-Lactate Dehydrogenase; D005947:Glucose; D002118:Calcium