Inhibition of oxidized low-density lipoprotein metabolism in macrophage J774 by helvolic acid.
The antibiotic helvolic acid inhibited cholesteryl ester accumulation in macrophage J774 treated with oxidized low-density lipoprotein (LDL) at a concentration of 50-350 microM. The agent reduced oxidized 125I-LDL degradation and [14C]oleate incorporation into cholesteryl ester. In a cell-free assay, ATP-dependent acidification of endosomes and lysosomes was significantly inhibited by 35 microM helvolic acid, suggesting that this activity accounts for the inhibition of oxidized LDL metabolism in the macrophages.
Shinohara
C
C
Department of Applied Biological Science, Tokyo Noko University, Japan.
Hasumi
K
K
Endo
A
A
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002250:Carbon Radioisotopes; D002460:Cell Line / Q000187:drug effects; D002474:Cell-Free System / Q000378:metabolism; D002788:Cholesterol Esters / Q000378:metabolism; D005672:Fusidic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007457:Iodine Radioisotopes; D007930:Leucine / Q000378:metabolism; D008077:Lipoproteins, LDL / Q000378:metabolism*; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D019301:Oleic Acid; D009829:Oleic Acids / Q000378:metabolism; D010084:Oxidation-Reduction; D014316:Tritium
D002250:Carbon Radioisotopes; D002788:Cholesterol Esters; D007457:Iodine Radioisotopes; D008077:Lipoproteins, LDL; D009829:Oleic Acids; D014316:Tritium; D019301:Oleic Acid; D005672:Fusidic Acid; D000255:Adenosine Triphosphate; D007930:Leucine; C011267:helvolic acid