[Characterization of autologous-tumor specific cytotoxic T cells induced by means of butanol-extracted soluble antigen from T lymphocytes in colon cancer patients].
Tumor-specific T lymphocytes (CTL) are proliferated in vitro to induce by a stimulation with butanol-extracted soluble antigen (CBE) and human recombinant interleukin-2 (IL-2) from peripheral lymphocytes of cancer patients. Peripheral blood T lymphocytes (PBL-T), tumor infiltrating lymphocytes (TIL) and lymph node lymphocytes (LNC) from patients with colon cancer were stimulated in vitro with CBE and IL-2. Single stimulation with CBE (1 x 10(-3) micrograms/ml) also activated the proliferative response of the PBL-T (p < 0.01). On the other hand, high-dose (0.25-2 micrograms/ml) significantly inhibited the proliferation of the PBL-T that had been stimulated with 20U/ml IL-2 (p < 0.001). The IL-2 receptor expression of PBL-T, TIL and LNC was also activated with low-dose CBE and IL-2. The surface markers of stimulated lymphocytes responded anti-CD3 and CD8 monoclonal antibodies, but some TIL and LNC displayed CD3 and CD4 phenotype. The lymphocytes responding anti-CD8 monoclonal antibody possessed the cytotoxic activity against autologous tumor cells, but not the lymphocytes responding anti-CD4 monoclonal antibody. The results suggested that low-dose CBE and IL-2 augment the IL-2 receptor expression of T lymphocytes, thereby the proliferation of the antigen specific cytotoxic T cells.
Hamagashira
K
K
Second Department of Surgery, Kyoto Prefectural University of Medicine, Japan.
D000951:Antigens, Neoplasm / Q000276:immunology* / Q000302:isolation & purification; D000954:Antigens, Surface / Q000032:analysis / Q000276:immunology; D000440:Butanols; D002455:Cell Division; D002478:Cells, Cultured; D003110:Colonic Neoplasms / Q000276:immunology*; D003601:Cytotoxicity Tests, Immunologic; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology; D015375:Receptors, Interleukin-2 / Q000032:analysis; D011994:Recombinant Proteins / Q000494:pharmacology; D013601:T-Lymphocytes / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014407:Tumor Cells, Cultured
D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D000440:Butanols; D007376:Interleukin-2; D015375:Receptors, Interleukin-2; D011994:Recombinant Proteins