title: Calbindin-D28k immunoreactivity and selective vulnerability to ischemia in the dentate gyrus of the developing rat.
abstract: Hippocampal dentate granule cells normally express the calcium-binding protein calbindin-D28k and, in the adult, are the hippocampal neurons least vulnerable to an ischemic insult. We evaluated hippocampal structure 2-3 days after hypoxic/ischemic insult at postnatal day 7-10, and discovered that, unlike adult granule cells, developing granule cells were irreversibly injured. Localization of calbindin-D28k-like immunoreactivity (LI) revealed that the vulnerable cells were the immature granule cells at the base of the cell layer that were not yet calbindin-immunoreactive. Adjacent granule cells that did not die in response to the hypoxic/ischemic insult were calbindin-immunoreactive. Whether the lack of calbindin-LI in immature granule cells is causally related to their vulnerability, or is a coincidental reflection of cellular immaturity, remains to be determined.
authors: Neurology Research Center, Helen Hayes Hospital, New York State Department of Health, West Haverstraw 10993.
authors: J H
authors: JH
authors: Goodman
authors: C G
authors: CG
authors: Wasterlain
authors: W F
authors: WF
authors: Massarweh
authors: E
authors: E
authors: Dean
authors: A L
authors: AL
authors: Sollas
authors: R S
authors: RS
authors: Sloviter
mesh_terms: D000818:Animals; D000831:Animals, Newborn / Q000254:growth & development / Q000378:metabolism; D002545:Brain Ischemia / Q000378:metabolism* / Q000473:pathology; D064092:Calbindin 1; D064026:Calbindins; D006624:Hippocampus / Q000378:metabolism* / Q000648:ultrastructure; D000860:Hypoxia / Q000378:metabolism / Q000473:pathology; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D008970:Molecular Weight; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D064030:S100 Calcium Binding Protein G / Q000737:chemistry / Q000378:metabolism*
chemical_list: C576500:Calb1 protein, rat; D064092:Calbindin 1; D064026:Calbindins; D064030:S100 Calcium Binding Protein G