title: Differential effects of anticytoskeletal compounds on the localization and chemical patterns of actin in germinating conidia of Neurospora crassa.
abstract: Anti-actin drugs, cytochalasins A and B, inhibited both normal single, and benomyl-induced multiple, germ tube outgrowth from conidia of Neurospora crassa. Actin was cytochemically found to be concentrated in each of the benomyl-induced germ tube tips. No significant quantitative changes either in total actin or its isoforms were measured in the inhibitor-treated germlings. While intact microtubules are required for normal, monopolar axiation of the germ tube, they appear not to be necessary for benomyl-induced multipolar outgrowth which, in contrast, still requires intact actin microfilaments. Microfilaments and microtubules thus play complementary roles in the normal germination of conidia.
authors: Laboratory of General Microbiology, University of Geneva, Switzerland.
authors: F
authors: F
authors: Barja
authors: M L
authors: ML
authors: Chappuis
authors: G
authors: G
authors: Turian
mesh_terms: D000199:Actins / Q000378:metabolism*; D001542:Benomyl / Q000494:pharmacology; D003571:Cytochalasin B / Q000494:pharmacology; D003572:Cytochalasins / Q000494:pharmacology; D003599:Cytoskeleton / Q000187:drug effects*; D006651:Histocytochemistry; D009492:Neurospora crassa / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism*
chemical_list: D000199:Actins; D003572:Cytochalasins; C031218:cytochalasin A; D003571:Cytochalasin B; D001542:Benomyl