Influence of blood storage after in vitro exposure on the yield of micronuclei observed in human lymphocytes.
In a cooperative study, four laboratories evaluated the micronucleus test in irradiated and non-irradiated lymphocytes with respect to time of storage, the difference between X-ray and gamma exposure and inter-observer variance. The results were compared with parallel studies on dicentric aberrations. No significant differences between laboratories, with respect to storage time or between gamma and X-irradiation were observed for micronuclei and dicentrics using analysis of variance. However, micronuclei are not suitable for an assessment of exposure inhomogeneity because of a significant overdispersion already in controls and homogeneously irradiated samples.
Lopez
I
I
Laboratorio Dosimetria Biologica, CEN La Reina, Santiago, Chile.
Hilali
A
A
Verschaeve
L
L
Laurent
C
C
Bugueno
C
C
Léonard
E D
ED
Maes
A
A
Jadot
P
P
Goens
G
G
Sanhueza
S
S
D001530:Belgium; D001793:Blood Preservation / Q000706:statistics & numerical data; D002677:Chile; D004307:Dose-Response Relationship, Radiation; D006801:Humans; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000528:radiation effects* / Q000648:ultrastructure; D008297:Male; D048629:Micronuclei, Chromosome-Defective / Q000528:radiation effects*; D015162:Micronucleus Tests / Q000706:statistics & numerical data; D015588:Observer Variation; D013997:Time Factors