title: [Simultaneous measurement of kinetochore protein and DNA by flow cytometry].
abstract: We were successful in obtaining simultaneous measurements of kinetochore protein and DNA with flow cytometry using HeLa cells and KM cells; the latter originated with a squamous cell carcinoma of the oral cavity. Kinetochore protein was stained with the serum obtained from a patient with CREST syndrome and FITC-conjugated goat antihuman IgG antibody; the DNA was stained with propidium iodide. The FITC fluorescence of cells with a large DNA index was stronger than in cells with a small DNA index. The FITC intensity increased from G1 phase to G2/M phase. The rate of FITC intensity increase representing the kinetochore protein amount was greater in the early and late S phases than in the other phases. The results suggest that kinetochore protein increases in the interphase as the cell cycle unfolds.
authors: Dept. of Pathology, Yamaguchi University School of Medicine.
authors: A
authors: A
authors: Oga
authors: T
authors: T
authors: Murakami
authors: Y
authors: Y
authors: Kimura
authors: M
authors: M
authors: Ida
authors: M
authors: M
authors: Takahashi
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000473:pathology; D002453:Cell Cycle; D002503:Centromere / Q000737:chemistry*; D002868:Chromosomal Proteins, Non-Histone / Q000032:analysis*; D004273:DNA, Neoplasm / Q000032:analysis*; D005434:Flow Cytometry; D006367:HeLa Cells; D006801:Humans; D009062:Mouth Neoplasms / Q000473:pathology
chemical_list: D002868:Chromosomal Proteins, Non-Histone; D004273:DNA, Neoplasm