The appearance of neutral lipid signals in the 1H NMR spectra of a myeloma cell line correlates with the induced formation of cytoplasmic lipid droplets.
The appearance of high resolution neutral lipid signals in the 1H NMR spectra of myeloma cells grown in the presence of oleate was shown to correlate with the appearance of cytoplasmic lipid droplets observable by electron microscopy. The spin-spin relaxation times of these lipid signals were similar to those measured previously for lipid resonances in other cell types. These data suggest that cytoplasmic lipid droplets could make a significant contribution to the neutral lipid signals observed in the 1H NMR spectra of some cells.
Callies
R
R
Department of Biochemistry and Molecular Biology, University of Manchester, UK.
Sri-Pathmanathan
R M
RM
Ferguson
D Y
DY
Brindle
K M
KM
D000818:Animals; D003593:Cytoplasm / Q000378:metabolism; D066298:In Vitro Techniques; D050356:Lipid Metabolism; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008854:Microscopy, Electron; D009101:Multiple Myeloma / Q000378:metabolism* / Q000473:pathology; D019301:Oleic Acid; D009829:Oleic Acids; D014407:Tumor Cells, Cultured
D009829:Oleic Acids; D019301:Oleic Acid