Early postnatal undernutrition impairs protein kinase C-dependent phosphorylation in rat brain synaptosomes.
Undernutrition in rat pups was established by restricting feeding time daily. Protein kinase C-dependent phosphorylation in vitro was studied by incubating the mitochondrial-synaptosomal membrane fractions from adult, 18-day-old control and undernourished rats with gamma-[32P]ATP in presence of Ca2+ and phosphatidylserine. In adult and 18-day-old control rats, an increased phosphorylation of three major proteins (49, 53, 84 kDa) were detected in presence of calcium and phosphatidylserine. However, in 18-day-old undernourished rats, calcium/phosphatidylserine activated phosphorylation was found to be significantly impaired with only a slightly increased labelling detected in the 49 kDa protein.
Chakrabarti
S
S
Department of Biochemistry, Goa Medical College, Bambolim, India.
Sonaye
B
B
Nadkarni
P P
PP
Da Silva
S V
SV
D000255:Adenosine Triphosphate / Q000378:metabolism*; D000375:Aging / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000254:growth & development / Q000378:metabolism*; D008565:Membrane Proteins / Q000302:isolation & purification / Q000378:metabolism*; D009748:Nutrition Disorders / Q000378:metabolism*; D010750:Phosphoproteins / Q000302:isolation & purification / Q000378:metabolism*; D010766:Phosphorylation; D011493:Protein Kinase C / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013574:Synaptosomes / Q000378:metabolism*
D008565:Membrane Proteins; D010750:Phosphoproteins; D000255:Adenosine Triphosphate; D011493:Protein Kinase C