Induction of hepatic polyamines by di(2-ethylhexyl)phthalate in rats.
Wistar male albino rats, weighting 125-150 g, were administered di(2-ethylhexyl)phthalate (DEHP) orally at 250, 500, 1000 and 2000 mg/kg body weight diluted in groundnut oil consecutively for 14 days. Spermidine and spermine of the livers were separated by thin-layer chromatography and quantitatively estimated by spectrofluorometry. DEHP exposure led to a significant increase in the absolute and relative weights of the liver at the 1000 and 2000 mg/kg dose levels. Spermidine and spermine levels were induced significantly in a dose-dependent manner. The increase in spermidine was greater as compared to that of spermine. Our results manifest that DEHP significantly induces the levels of hepatic polyamines.
Khaliq
M A
MA
Industrial Toxicology Research Centre, Lucknow, India.
Srivastava
S P
SP
D000284:Administration, Oral; D000818:Animals; D002855:Chromatography, Thin Layer; D004051:Diethylhexyl Phthalate / Q000008:administration & dosage / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013095:Spermidine / Q000096:biosynthesis* / Q000302:isolation & purification; D013096:Spermine / Q000096:biosynthesis* / Q000302:isolation & purification
D013096:Spermine; D004051:Diethylhexyl Phthalate; D013095:Spermidine