Synthesis of [35S]thiophosphoryl adenylic acid, utilizing a general procedure for [35S]thiophosphoryl chloride production.
Elemental [35S]sulfur was shown to equilibrate with the sulfur of thiophosphoryl chloride when these materials are heated together. This isotopic exchange reaction is the basis of a convenient, microscale synthesis of high specific activity [35S]PSCl3. [35S]Thiophosphoryl chloride is otherwise not commercially available except through custom synthesis. The labeled thiophosphoryl chloride was used in a novel procedure for the preparation of [35S]adenosine 5'-phosphorothioate. This isotopic exchange method should find wide application in the synthesis of many radiolabeled thiophosphoryl esters which utilize PSCl3 as the source of the thiophosphoryl group.
Slama
J T
JT
Department of Biochemistry, University of Texas Health Science Center, San Antonio 78284-7760.
Simmons
A M
AM
Hernandez
T M
TM
Keenan
R W
RW
D000249:Adenosine Monophosphate / Q000031:analogs & derivatives* / Q000138:chemical synthesis; D002712:Chlorides / Q000737:chemistry*; D007553:Isotope Labeling / Q000379:methods*; D007700:Kinetics; D010758:Phosphorus / Q000737:chemistry*; D017553:Phosphorus Compounds; D013455:Sulfur / Q000737:chemistry; D013462:Sulfur Radioisotopes / Q000737:chemistry*; D013873:Thionucleotides / Q000138:chemical synthesis*
D002712:Chlorides; D017553:Phosphorus Compounds; D013462:Sulfur Radioisotopes; D013873:Thionucleotides; C014308:adenosine 5'-phosphorothioate; D010758:Phosphorus; D000249:Adenosine Monophosphate; D013455:Sulfur; C043261:thiophosphoryl chloride