Granulocyte aggregation is edetic acid and temperature dependent.
Nineteen cases of leukocyte agglutination were observed in the last 6 years, while performing routine blood cell counts with an autoanalyzer (Technicon H6000). The phenomenon occurred only in edetic acid-anticoagulated samples and only at room temperature. Leukocyte aggregation could be reproduced by incubating patient serum samples or edetic acid-plasma with whole blood from ABO-compatible healthy control subjects. When patient serum samples or plasma samples were first preincubated with anti-IgM serum and then mixed with normal blood, leuko-agglutinates were not observed, suggesting that a plasma factor, probably an IgM, was responsible for the phenomenon. Nonetheless, IgM presence could not be correlated with any particular disease or the use of specific drugs.
Bizzaro
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N
Laboratory of Clinical Pathology, Ospedale Civile, S. Doná di Piave, Venice, Italy.
D000328:Adult; D000368:Aged; D001772:Blood Cell Count; D002449:Cell Aggregation / Q000187:drug effects / Q000502:physiology; D004492:Edetic Acid / Q000494:pharmacology*; D005260:Female; D006098:Granulocytes / Q000166:cytology* / Q000502:physiology; D006801:Humans; D007075:Immunoglobulin M / Q000502:physiology; D008297:Male; D008875:Middle Aged; D013696:Temperature; D013997:Time Factors
D007075:Immunoglobulin M; D004492:Edetic Acid