1H-detected in vivo 13C NMR spectroscopy and imaging at 2T magnetic field: efficient monitoring of 13C-labeled metabolites in the rat brain derived from 1-13C-glucose.
Gradient-select hetero-nuclear multiple quantum coherence in nuclear magnetic resonance (NMR) an indirect method of detecting nuclei in low sensitivity by 1H NMR, was used to observe 13C-enriched metabolites in the rat brain in vivo, with a 2 T NMR system. We detected the accumulation of 13C-labeled glucose and its metabolites, glutamine and/or glutamate, in less than 5 min. The extension of this technique to chemical shift imaging permitted the localization of these chemicals in the rat brain. This approach may have various applications for non-invasive monitoring of 13C-labeled compounds in humans and other animals.
Inubushi
T
T
Molecular Neurobiology Research Center, Shiga University of Medical Science, Japan.
Morikawa
S
S
Kito
K
K
Arai
T
T
D000818:Animals; D001921:Brain / Q000378:metabolism*; D004734:Energy Metabolism; D005947:Glucose / Q000378:metabolism*; D005971:Glutamates / Q000378:metabolism; D005973:Glutamine / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D051381:Rats; D017208:Rats, Wistar
D005971:Glutamates; D005973:Glutamine; D005947:Glucose