Determination of tumor aggressiveness in colorectal cancer by K-ras-2 analysis.
Markers that predict tumor aggressiveness on a case-by-case basis would enable individualization and optimization of oncologic therapy. To achieve this goal, the presence and specific type of K-ras-2 point mutation was determined from formalin-fixed, paraffin-embedded tissue sites in 247 primary and 166 metastatic-recurrent colorectal adenocarcinomas, using a novel approach consisting of topographic tissue selection, DNA amplification, and direct sequencing applicable to large and needle-biopsy-sized specimens. The results provide the basis for a genotypic classification of colorectal cancer capable of predicting individual tumor aggressiveness, including the pattern and extent of metastasis.
Finkelstein
S D
SD
Department of Pathology, Rhode Island Hospital, Brown University, Providence 02903.
Sayegh
R
R
Bakker
A
A
Swalsky
P
P
D000230:Adenocarcinoma / Q000235:genetics / Q000473:pathology* / Q000556:secondary; D001224:Aspartic Acid / Q000235:genetics; D014408:Biomarkers, Tumor / Q000235:genetics; D003062:Codon / Q000235:genetics; D003110:Colonic Neoplasms / Q000235:genetics* / Q000473:pathology*; D004273:DNA, Neoplasm / Q000235:genetics; D005091:Exons / Q000235:genetics; D005500:Follow-Up Studies; D011905:Genes, ras / Q000235:genetics*; D005973:Glutamine / Q000235:genetics; D006639:Histidine / Q000235:genetics; D006801:Humans; D009154:Mutation / Q000235:genetics*; D009361:Neoplasm Invasiveness / Q000235:genetics; D009364:Neoplasm Recurrence, Local; D009367:Neoplasm Staging; D012004:Rectal Neoplasms / Q000235:genetics* / Q000473:pathology*; D012189:Retrospective Studies; D015996:Survival Rate; D014633:Valine / Q000235:genetics
D014408:Biomarkers, Tumor; D003062:Codon; D004273:DNA, Neoplasm; D005973:Glutamine; D001224:Aspartic Acid; D006639:Histidine; D014633:Valine