title: The reaction with polytene chromosomes of antibodies raised against Drosophila E75A protein.
abstract: The steroid insect molting hormone ecdysone rapidly induces a small number of polytene chromosome puffs in Drosophila. The Ashburner model proposes that the corresponding early genes encode proteins involved in both the induction of the late genes and the repression of the early genes. The Drosophila E75 early gene has been isolated and two of its products, E75A and E75B, have been shown to be members of the steroid receptor superfamily. We have now prepared antisera directed against A- and B-specific regions of the E75 proteins. Antisera and a monoclonal antibody raised against E75A, the major larval protein product of the E75 gene, bind to discrete sites in native salivary gland chromosomes. These sites are closely correlated with early and late ecdysone responsive loci.
authors: CSIRO Division of Biomolecular Engineering, Laboratory for Molecular Biology, North Ryde, NSW, Australia.
authors: R J
authors: RJ
authors: Hill
authors: W A
authors: WA
authors: Segraves
authors: D
authors: D
authors: Choi
authors: P A
authors: PA
authors: Underwood
authors: E
authors: E
authors: Macavoy
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D001665:Binding Sites; D004268:DNA-Binding Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000235:genetics / Q000378:metabolism*; D004440:Ecdysone / Q000378:metabolism; D007150:Immunohistochemistry
chemical_list: D000911:Antibodies, Monoclonal; D004268:DNA-Binding Proteins; D004440:Ecdysone