Sulfite inhibits oxalate production from glycolate and glyoxylate in vitro and from dichloroacetate infused i.v. into male rats.
The effect of sulfite on oxalate production from glycolate and glyoxylate was investigated in an in vitro assay system using rabbit muscle LDH and rat liver 35-60% ammonium sulfate fraction as enzymes. A > or = 50% inhibition in oxalate production was observed at sulfite to glyoxylate ratio of 0.4. LDH activity (change in A340 nm) was inhibited by > or = 40% at sulfite to glyoxylate ratio of 0.5. Male Sprague-Dawley rats fasted for 24 h and infused with dichloroacetate (150 mg/rat/h, iv, n = 6), without and with sodium sulfite (150 mg/rat/h, n = 6) excreted oxalate in urine, respectively, at the rate of 1.13 +/- 0.29 and 0.61 +/- 0.09 mumol/h/100 g body wt, showing a nearly 50% reduction due to sulfite. The present report demands further experimentation to define the role of sulfite as an intracellular modulator of oxalate production.
Sharma
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Division of Experimental Surgery, University of Erlangen, Germany.
Schwille
P O
PO
D000429:Alcohol Oxidoreductases / Q000378:metabolism; D000818:Animals; D003999:Dichloroacetic Acid / Q000494:pharmacology*; D006016:Glycolates / Q000378:metabolism*; D006038:Glyoxylates / Q000378:metabolism*; D007770:L-Lactate Dehydrogenase / Q000037:antagonists & inhibitors / Q000378:metabolism; D008099:Liver / Q000201:enzymology; D008297:Male; D009132:Muscles / Q000201:enzymology; D010070:Oxalates / Q000378:metabolism*; D019815:Oxalic Acid; D011817:Rabbits; D051381:Rats; D017207:Rats, Sprague-Dawley; D013447:Sulfites / Q000494:pharmacology*
D006016:Glycolates; D006038:Glyoxylates; D010070:Oxalates; D013447:Sulfites; C031149:glycolic acid; D019815:Oxalic Acid; D003999:Dichloroacetic Acid; D000429:Alcohol Oxidoreductases; D007770:L-Lactate Dehydrogenase; C020821:glycollate oxidase; C031150:glyoxylic acid; C025026:sodium sulfite