Comparison of COS cell transfected AT1A and AT1B angiotensin II receptors and angiotensin II receptor isoforms in rat tissues using isoelectric focusing.
Rat adrenal AT1A and AT1B receptors from transfected COS-7 cells were labelled with 125I-Angiotensin II, solubilised, and run on isoelectric focusing gels. Receptors from rat tissues were treated similarly. COS-7 cell-expressed AT1A and AT1B receptors each produced a single peak of specific radioactivity at pI 6.8. Rat liver and rat ovary tissue preparations gave peaks at pI 6.8 and 6.5, respectively. In contrast, rat adrenal tissue preparations gave four peaks at pI 7.0, 6.8, 6.5, and 6.3. The additional isoforms found in the rat adrenal tissue preparations may represent post-translationally modified or novel receptors.
Barker
S
S
Department of Biochemistry, Faculty of Basic Medical Sciences, Queen Mary and Westfield College, London, U.K.
Marchant
W
W
Clark
A J
AJ
Jimenez
E
E
Marsigliante
S
S
Montiel
M
M
Vinson
G P
GP
D000311:Adrenal Glands / Q000737:chemistry; D000818:Animals; D002460:Cell Line; D005260:Female; D007525:Isoelectric Focusing; D008099:Liver / Q000737:chemistry; D010053:Ovary / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D011945:Receptors, Angiotensin / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D014162:Transfection
D011945:Receptors, Angiotensin