Interactions of anti-DNA antibodies with Z-DNA.
Systemic lupus erythematosus (SLE) sera, two classes of serum lipoproteins, and IgG antibodies from SLE and normal sera were tested for their reactivity with a Z-DNA polymer, Br-poly (dG-dC). In all cases preferential binding to Z-DNA over B-DNA was observed. This interaction, for the most part, could be inhibited by the negatively charged phospholipid, cardiolipin, which suggests that most of the anti-Z-DNA activity associated with sera arises from relatively non-specific ionic interactions between proteins and polyanionic molecules. An assay has been described that can eliminate proteins cross-reactive with negatively charged phospholipids.
Krishna
P
P
Department of Medical Biochemistry, University of Calgary, Alberta, Canada.
Fritzler
M J
MJ
Van de Sande
J H
JH
D000974:Antibodies, Antinuclear / Q000378:metabolism*; D001667:Binding, Competitive; D002308:Cardiolipins / Q000502:physiology; D003429:Cross Reactions; D004247:DNA / Q000378:metabolism*; D004277:DNA, Single-Stranded / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000097:blood; D004305:Dose-Response Relationship, Drug; D005997:Glycerylphosphorylcholine / Q000494:pharmacology; D006801:Humans; D015151:Immunoblotting; D007074:Immunoglobulin G / Q000378:metabolism*; D008074:Lipoproteins / Q000378:metabolism; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*; D010712:Phosphatidic Acids / Q000494:pharmacology; D010713:Phosphatidylcholines / Q000494:pharmacology
D000974:Antibodies, Antinuclear; D002308:Cardiolipins; D004277:DNA, Single-Stranded; D004268:DNA-Binding Proteins; D007074:Immunoglobulin G; D008074:Lipoproteins; D010712:Phosphatidic Acids; D010713:Phosphatidylcholines; D005997:Glycerylphosphorylcholine; D004247:DNA