title: NO- and NO2-carrying molecules potentiate photorelaxation in rat trachea and aorta.
abstract: Photorelaxation elicited by ultraviolet light (366 nm) was investigated on isolated rat thoracic aorta and trachealis. Rat tracheal smooth muscle but not aorta did not show UV-induced photorelaxation. Both streptozotocin, NO-carrying molecule and N omega-nitro-L-arginine, NO2-carrying molecule significantly enhanced photorelaxation, concentration-dependently, in rat trachealis and aorta. Methylene blue (10 microM) inhibited the potentiation action of streptozotocin and N omega-nitro-L-arginine in both tissues. Superoxide dismutase (300 U/ml) enhanced streptozotocin- and N omega-nitro-L-arginine-potentiated photorelaxation in rat trachealis, while pyrogallol (0.1 mM), a potent O2- generating agent, inhibited streptozotocin-potentiated photorelaxation in trachealis. Streptozotocin was much more effective than N omega-nitro-L-Arginine in potentiating of photorelaxation elicited by UV light in both tissues. From these findings, we conclude that streptozotocin and N omega-nitro-L-arginine produce EDRF like labile substance(s) by UV irradiation.
authors: Department of Pharmacology, Gyeongsang National University, Chinju, Korea.
authors: K C
authors: KC
authors: Chang
authors: W S
authors: WS
authors: Chong
authors: B W
authors: BW
authors: Park
authors: B W
authors: BW
authors: Seung
authors: G W
authors: GW
authors: Chun
authors: I J
authors: IJ
authors: Lee
authors: P S
authors: PS
authors: Park
mesh_terms: D000818:Animals; D001011:Aorta; D001120:Arginine / Q000031:analogs & derivatives / Q000494:pharmacology; D005260:Female; D066298:In Vitro Techniques; D008297:Male; D008751:Methylene Blue / Q000494:pharmacology; D009126:Muscle Relaxation / Q000187:drug effects* / Q000528:radiation effects; D009130:Muscle, Smooth / Q000187:drug effects* / Q000502:physiology / Q000528:radiation effects; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects; D009569:Nitric Oxide / Q000494:pharmacology*; D019335:Nitroarginine; D009609:Nitrous Oxide / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013311:Streptozocin / Q000494:pharmacology; D013482:Superoxide Dismutase / Q000378:metabolism; D014132:Trachea; D014466:Ultraviolet Rays
chemical_list: D019335:Nitroarginine; D009569:Nitric Oxide; D013311:Streptozocin; D001120:Arginine; D013482:Superoxide Dismutase; D009609:Nitrous Oxide; D008751:Methylene Blue