[Effect of 5H-alpha-cholest-8(14)-en-3-beta-ol-15-one on cholesterol metabolism in cultured rabbit hepatocytes].
The effects of 15-ketosterol on cholesterol metabolism in cultured rabbit hepatocytes were characterized by the following parameters: a) cholesterol synthesis, b) apo B and apo E secretion, c) bile acid synthesis. 15-Ketosterol used at therapeutic concentration (0.25 microM) reduced cholesterol synthesis (by 50%). Marked inhibition (by 70%) of apo B and apo E secretion was observed for this agent. Synthesis and secretion of the total [14C]-labeled protein remaining unchanged. 15-Ketosterol did not influence the bile acid synthesis in primary culture of rabbit hepatocytes. These results are suggestive of a new putative mechanism of hyperlipidemic action of 15-ketosterol by a simultaneous decrease of hepatic cholesterol synthesis and secretion of apo B-containing particles.
Mambetisaeva
E T
ET
Kosykh
V A
VA
Misharin
A Iu
AIu
Maliugin
A V
AV
Kosenkov
E I
EI
Podrez
E A
EA
Repin
V S
VS
D000818:Animals; D001055:Apolipoproteins B / Q000378:metabolism; D001057:Apolipoproteins E / Q000378:metabolism; D001647:Bile Acids and Salts / Q000096:biosynthesis; D002478:Cells, Cultured; D002783:Cholestenones / Q000494:pharmacology*; D002784:Cholesterol / Q000096:biosynthesis / Q000378:metabolism*; D008099:Liver / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D011817:Rabbits
D001055:Apolipoproteins B; D001057:Apolipoproteins E; D001647:Bile Acids and Salts; D002783:Cholestenones; C015073:cholest-8(14)-en-3-ol-15-one; D002784:Cholesterol
Vliianie 5-H-alpha-kholest-8(14)-en-3-beta-ol-15-ona na metabolizm kholesterina v kul'tiviruemykh gepatotsitakh krolika.