Isolation and characterization of a new spore-forming sulfate-reducing bacterium growing by complete oxidation of catechol.
A new mesophilic sulfate-reducing bacterium, strain Groll, was isolated from a benzoate enrichment culture inoculated with black mud from a freshwater ditch. The isolate was a spore-forming, rod-shaped, motile, gram-positive bacterium. This isolate was able of complete oxidation of several aromatic compounds including phenol, catechol, benzoate, p- and m-cresol, benzyl alcohol and vanillate. With hydrogen and carbon dioxide, formate or O-methylated aromatic compounds, autotrophic growth during sulfate reduction or homoacetogenesis was demonstrated. Lactate was not used as a substrate. SO4(2-), SO3(2-), and S2O3(2-) were utilized as electron acceptors. Although strain Groll originated from a freshwater habitat, salt concentrations of up to 30 g.l-1 were tolerated. The optimum temperature for growth was 35-37 degrees C. The G + C content of DNA was 42.1 mol%. This isolate is described as a new species of the genus Desulfotomaculum.
Kuever
J
J
AG Mikrobiologie, Universität Oldenburg, Germany.
Kulmer
J
J
Jannsen
S
S
Fischer
U
U
Blotevogel
K H
KH
D001406:Bacillaceae / Q000254:growth & development / Q000302:isolation & purification / Q000502:physiology*; D001673:Biodegradation, Environmental; D002396:Catechols / Q000378:metabolism*; D006838:Hydrocarbons / Q000378:metabolism; D010084:Oxidation-Reduction; D019800:Phenol; D010636:Phenols / Q000378:metabolism; D012988:Soil Microbiology; D013171:Spores, Bacterial / Q000502:physiology; D013431:Sulfates / Q000378:metabolism
D002396:Catechols; D006838:Hydrocarbons; D010636:Phenols; D013431:Sulfates; D019800:Phenol; C034221:catechol