Comparative multiple synthesis of fifty linear peptides: evaluation of cotton carrier vs. T bag-benzhydrylamine resin.
Parallel simultaneous synthesis of fifty linear peptides has been carried out in order to compare in detail two promising methodologies of simultaneous multiple peptide synthesis (SMPS): the "T bag" method, utilizing 4-methyl-benzhydrylamine resin (MeBHA), and synthesis on derivatized Fmoc-Gly-O-cotton fabric strips. The basic set of experiments, which utilizes identical Fmoc/Bu(t) strategy for both approaches, shows that the peptides synthesized on cotton are superior in purity to those synthesized using T bags. In experiments utilizing Boc/Bzl strategy in T bags, the purities of peptides were higher than in the case of peptides synthesized in T bags by Fmoc/Bu(t) strategy, and comparable with the purities achieved in synthesis performed on cotton. The lower yields on cotton are caused by mechanical losses in the given experimental arrangement.
Rinnová
M
M
Institute of Organic Chemistry and Biochemistry, Czechoslovak Academy of Sciences.
Jezek
J
J
Malon
P
P
Lebl
M
M
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000737:chemistry; D001559:Benzhydryl Compounds / Q000737:chemistry; D002116:Calcitonin / Q000737:chemistry; D005449:Fluorenes / Q000737:chemistry; D005563:Formic Acid Esters / Q000737:chemistry; D005755:Gastrins / Q000737:chemistry; D003368:Gossypium / Q000737:chemistry; D007301:Insect Hormones / Q000737:chemistry; D009074:Melanocyte-Stimulating Hormones / Q000737:chemistry; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000737:chemistry; D009842:Oligopeptides / Q000138:chemical synthesis* / Q000737:chemistry / Q000302:isolation & purification; D010455:Peptides / Q000737:chemistry; D011761:Pyrrolidonecarboxylic Acid / Q000031:analogs & derivatives
C030545:4-methylbenzhydrylamine resin; D000596:Amino Acids; D001559:Benzhydryl Compounds; D005449:Fluorenes; D005563:Formic Acid Esters; D005755:Gastrins; D007301:Insect Hormones; C016456:N(alpha)-fluorenylmethyloxycarbonylamino acids; D009479:Neuropeptides; D009842:Oligopeptides; D010455:Peptides; C044833:adipokinetic hormone; C000345:t-butyloxycarbonyl group; C076523:allatostatin; C000058:alytesin; D009074:Melanocyte-Stimulating Hormones; D002116:Calcitonin; D011761:Pyrrolidonecarboxylic Acid