The large cytoplasmic domain is not required for concentration of N-CAM at cell-cell contacts in transfected mouse neuroblastoma cells.
We examined the localization of the 140- and 180-kDa transmembrane isoforms of chicken N-CAM following transfection into mouse N2A neuroblastoma cells. Both isoforms were expressed at the cell surface and became partially or completely localized at areas of cell-cell contact after several days of culture or of in vitro differentiation. These results indicate that the presence of the large cytoplasmic domain of the 180-kDa N-CAM isoform is not necessary to bring about the localization of N-CAM to points of cell-cell contact.
Woo
M K
MK
Department of Developmental and Cell Biology, University of California Irvine 92717.
Kil
S H
SH
Adams
L D
LD
Nguyen
T N
TN
Murray
B A
BA
D000818:Animals; D002448:Cell Adhesion; D015816:Cell Adhesion Molecules, Neuronal / Q000737:chemistry* / Q000378:metabolism; D002645:Chickens; D003593:Cytoplasm / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D066298:In Vitro Techniques; D051379:Mice; D009447:Neuroblastoma; D013329:Structure-Activity Relationship; D014162:Transfection; D014407:Tumor Cells, Cultured
D015816:Cell Adhesion Molecules, Neuronal