title: Sequence analysis suggests a recent duplication of the growth hormone-encoding gene in Tilapia nilotica.
abstract: The sequence of two growth hormone(GH)-encoding genes from tilapia fish (Tilapia nilotica) is reported. Our data indicate that the presence of two GH in the tilapia genome is a consequence of a relatively recent duplication event. The two genes are highly homologous, having a similar intron (five)/exon (six) arrangement, and both encode an identical polypeptide. Sequence similarity extends up to bp -628 upstream to the transcription start point, after which the sequences of the two genes are not related to each other. The presence of two GH in the tilapia genome is supported both by the nucleotide sequence and by genomic DNA blot hybridization analysis. Tilapias, like salmonids, contain an extra intron compared with the mammalian GH structure. We suggest that within the superorder Teleostei, the insertion of intron 5 into GH took place after the evolutionary separation of Cyprinoidea, but before Isospondyli (salmonids) and Acanthopterygii (tilapias) were separated. Thus, the additional intron which is probably present in many teleost fish GH may provide an excellent natural marker for evolution and classification studies.
authors: Department of Biochemistry, Weizmann Institute of Science, Rehovot, Israel.
authors: R
authors: R
authors: Ber
authors: V
authors: V
authors: Daniel
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D017124:Conserved Sequence; D004247:DNA; D013006:Growth Hormone / Q000235:genetics*; D008969:Molecular Sequence Data; D005810:Multigene Family; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D017210:Tilapia / Q000235:genetics*
chemical_list: D013006:Growth Hormone; D004247:DNA