title: Thickness determination of biological thin sections by multiple-least-squares fitting of the carbon K-edge in the electron energy-loss spectrum.
abstract: We show that the local thickness of biological thin sections can be measured by fitting the electron energy-loss spectrum of the plurally scattered carbon K-edge, and that such measurements are consistent with the results obtained by a conventional method based on the relationship t/lambda alpha ln(It/I0). The new method allows the simultaneous measurement and/or mapping of both local thickness and core-level excitations (e.g. Ca L-edge) in this energy range (280-400 eV) without requiring the measurement of the zero-loss and the valence/plasmon excitations.
authors: Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville 22908.
authors: L
authors: L
authors: Zhao
authors: Y Y
authors: YY
authors: Wang
authors: R
authors: R
authors: Ho
authors: Z
authors: Z
authors: Shao
authors: A V
authors: AV
authors: Somlyo
authors: A P
authors: AP
authors: Somlyo
mesh_terms: D000818:Animals; D002244:Carbon; D004577:Electron Probe Microanalysis / Q000379:methods*; D016018:Least-Squares Analysis; D051381:Rats
chemical_list: D002244:Carbon