Identification of Yersinia pestis with varied plasmid composition using monoclonal and polyclonal fluorescent immunoglobulins.
The efficiency of serological identification of Yersinia pestis strains which contain different plasmids was assessed with polyclonal and monoclonal immunoglobulin preparations in the direct fluorescent antibody method. Plague polyclonal luminescent immunoglobulins recognize only those Y. pestis strains which contain pPst, pFra plasmids or both. Anticapsular plague monoclonal antibodies interact only with capsule-forming plague agent strains (pFra+) grown at 37 degrees C. With plague monoclonal lipopolysaccharide antibodies one can identify all Y. pestis strains irrespective of their plasmid content and cultivation temperature. However, these antibodies cross-react with Yersinia pseudotuberculosis bacteria in 60% of cases. The problem of laboratory diagnosis of the plague organism, whatever its plasmid profile, can be solved through the development of a test kit involving two preparations such as plague lipopolysaccharide monoclonal luminescent antibodies and pseudotuberculosis specific luminescent adsorbed immunoglobulins.
Devdariani
Z L
ZL
Russia State Antiplague Research Institute Microbe, Saratov.
Verenkov
M S
MS
Feodorova
V A
VA
Solodovnicov
N S
NS
Belov
L G
LG
D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D000918:Antibody Specificity; D003429:Cross Reactions; D016650:Fluorescein-5-isothiocyanate; D005455:Fluorescent Antibody Technique; D006736:Horses; D007136:Immunoglobulins / Q000276:immunology; D010957:Plasmids; D015010:Yersinia pestis / Q000145:classification* / Q000235:genetics / Q000276:immunology; D015011:Yersinia pseudotuberculosis / Q000276:immunology
D000907:Antibodies, Bacterial; D007136:Immunoglobulins; D016650:Fluorescein-5-isothiocyanate