title: Estrogen-inducible and liver-specific expression of the chicken Very Low Density Apolipoprotein II gene locus in transgenic mice.
abstract: We have examined the chicken Very Low Density Apolipoprotein II (apoVLDL II) gene locus in transgenic mice. A DNA fragment composed of the transcribed region, 16 kb of 5' flanking and 400 bp of 3' flanking sequences contained all the information sufficient for estrogen-inducible, liver-specific expression of the apoVLDL II gene. The far-upstream region contains a Negative Regulating Element coinciding with a DNaseI-hypersensitive site at -11 kb. In transgenic mice, the NRE at -11 kb is used for downregulating the expression to a lower maximum level. The NRE might be used for modulating apoVLDL II gene expression, and may be involved in the rapid shut-down of the expression after hormone removal.
authors: Laboratory of Biochemistry, Groningen University, The Netherlands.
authors: J
authors: J
authors: Wijnholds
authors: S
authors: S
authors: Philipsen
authors: S
authors: S
authors: Pruzina
authors: P
authors: P
authors: Fraser
authors: F
authors: F
authors: Grosveld
authors: G
authors: G
authors: Ab
mesh_terms: D000818:Animals; D001053:Apolipoproteins / Q000235:genetics*; D001483:Base Sequence; D002645:Chickens; D003850:Deoxyribonuclease I; D004967:Estrogens / Q000502:physiology; D005786:Gene Expression Regulation; D008079:Lipoproteins, VLDL / Q000235:genetics*; D008099:Liver / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D009928:Organ Specificity; D012045:Regulatory Sequences, Nucleic Acid
chemical_list: D001053:Apolipoproteins; D004967:Estrogens; D008079:Lipoproteins, VLDL; C027610:apolipoprotein VLDL II; D003850:Deoxyribonuclease I