Effect of cyclosporin A and its metabolites and analogs on lipid peroxidation in rabbit renal microsomes.
Rabbit renal microsomes were used to investigate the effect on lipid peroxidation (LPO) of cyclosporin A (CsA) and its first generation metabolites M1, M17 and M21, and of two natural CsA analogs: cyclosporins C and D (CsC & CsD respectively), at concentrations of 1 to 10 micrograms/ml. No induction of lipid peroxidation was observed with these substances except CsA at a high level (10 microgram/ml); but at a concentration of CsA that would be reached in vivo, no inductive effect on LPO was noted. A moderate inhibitory effect upon LPO was observed with CsC and M21, but its biological significance is questionable.
Sadeg
N
N
Laboratoire de Toxicologie, Faculté de Pharmacie, Paris.
Pham Huy
C
C
Martin
C
C
Warnet
J M
JM
Claude
J R
JR
D000818:Animals; D016572:Cyclosporine / Q000378:metabolism / Q000494:pharmacology*; D003524:Cyclosporins / Q000378:metabolism / Q000494:pharmacology*; D005260:Female; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism; D007700:Kinetics; D015227:Lipid Peroxidation / Q000187:drug effects*; D008315:Malondialdehyde / Q000378:metabolism; D008861:Microsomes / Q000187:drug effects* / Q000378:metabolism; D011817:Rabbits
D003524:Cyclosporins; D008315:Malondialdehyde; D016572:Cyclosporine