The effect of alpha-interferon on thymidine, uridine, and leucine uptake and ultrastructure of peripheral blood mononuclear cells from multiple myeloma patients.
The effect of IFN alpha-2b on thymidine, uridine, and leucine uptake was examined on peripheral blood mononuclear cells (PBMC) of healthy donors and 15 patients with multiple myeloma (MM). In addition, the surface ultrastructure of the cells incubated without or with IFN alpha-2b was examined with a scanning electron microscope. The results showed that IFN had no effect on thymidine, uridine, or leucine uptake of unstimulated MM and control PBMC. On the other hand IFN inhibited thymidine, and uridine uptake of PWM-stimulated MM PBMC, but had no effect on healthy donor stimulated PBMC. IFN inhibited also thymidine and uridine uptake in PHA-stimulated healthy donors and MM patients' PBMC. The cellular surface ultrastructure of MM lymphocytes incubated with 100 u/ml IFN showed disappearance of the microvilli and formation of cellular pits, whereas in healthy donor lymphocytes IFN caused flattening of microvilli.
Winder
A
A
Department of Medicine B, Golda Medical Center, Petah-Tikva, Sackler School of Medicine, Tel-Aviv University, Israel.
Fishman
P
P
Djaldetti
M
M
Cohen
A M
AM
D000368:Aged; D005260:Female; D006801:Humans; D000077190:Interferon alpha-2; D016898:Interferon-alpha / Q000494:pharmacology*; D007930:Leucine / Q000097:blood*; D007963:Leukocytes, Mononuclear / Q000378:metabolism / Q000648:ultrastructure*; D008214:Lymphocytes / Q000648:ultrastructure; D008297:Male; D008855:Microscopy, Electron, Scanning; D009101:Multiple Myeloma / Q000097:blood*; D011994:Recombinant Proteins; D013936:Thymidine / Q000097:blood*; D014529:Uridine / Q000097:blood*
D000077190:Interferon alpha-2; D016898:Interferon-alpha; D011994:Recombinant Proteins; D007930:Leucine; D013936:Thymidine; D014529:Uridine