Determination of nicotine and two major metabolites in serum by solid-phase extraction and high-performance liquid chromatography, and high-performance liquid chromatography-particle beam mass spectrometry.
A rapid and selective assay of nicotine, cotinine and trans-3'-hydroxycotinine in human serum, based on high-performance liquid chromatography with UV detection has been developed. The compounds were subjected to solid-phase extraction, using Extrelut 1 cartridges. Recoveries were ca. 95% for nicotine, 90% for cotinine and 50-55% for trans-3'-hydroxycotinine. The limit of quantitation observed with this method was 10 ng/ml for nicotine and 5 ng/ml for each of the metabolites. The compounds were also identified using high-performance liquid chromatography with particle beam mass spectrometry, to confirm their presence in human serum.
Pacifici
R
R
Ospedale Santo Spirito, Rome, Italy.
Pichini
S
S
Altieri
I
I
Rosa
M
M
Bacosi
A
A
Caronna
A
A
Zuccaro
P
P
D002851:Chromatography, High Pressure Liquid; D003367:Cotinine / Q000031:analogs & derivatives / Q000097:blood; D006801:Humans; D013058:Mass Spectrometry; D009538:Nicotine / Q000097:blood*; D012015:Reference Standards; D012907:Smoking / Q000097:blood; D013056:Spectrophotometry, Ultraviolet
C001381:hydroxycotinine; D009538:Nicotine; D003367:Cotinine