Hydrolysis of fish oils containing polymers of triacylglycerols by pancreatic lipase in vitro.
Fish oils containing different levels of polymers of triacylglycerols formed during autoxidation were incubated with pancreatic lipase to establish whether these polymers are substrates for lipase hydrolysis. With oils containing low amounts (less than 4%) of triacylglycerol polymers as substrates, both triacylglycerols and polymers of triacylglycerols were almost completely hydrolyzed, and fatty acid monomers and monoacylglycerols were the major lipid products. Under the same incubation conditions, some triacylglycerols remained intact when highly oxidized oils containing 20 or 30% triacylglycerol polymers were the substrate. The fatty acid composition of these residual triacylglycerols was almost identical to that of triacylglycerols present at the start of the assay. When fish oil containing 30% triacylglycerol polymers was incubated with the lipase, the component triacylglycerols and polymers of triacylglycerols were hydrolyzed at similar rates, and fatty acid dimers were detected as a product. It is concluded that the high molecular weight polymers of triacylglycerols present in oxidized fish oils can be hydrolyzed by pancreatic lipase in vitro.
Henderson
R J
RJ
N.E.R.C. Unit of Aquatic Biochemistry, Department of Biological and Molecular Science, University of Stirling, Scotland, United Kingdom.
Burkow
I C
IC
Millar
R M
RM
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D003060:Cod Liver Oil / Q000378:metabolism*; D005227:Fatty Acids / Q000032:analysis; D006868:Hydrolysis; D008049:Lipase / Q000378:metabolism*; D010084:Oxidation-Reduction; D010179:Pancreas / Q000201:enzymology; D011108:Polymers / Q000378:metabolism*; D013379:Substrate Specificity; D013552:Swine; D014001:Tin; D017971:Tin Compounds; D014280:Triglycerides / Q000737:chemistry / Q000378:metabolism*
D005227:Fatty Acids; D011108:Polymers; D017971:Tin Compounds; D014280:Triglycerides; C023599:stannous chloride; D014001:Tin; D003060:Cod Liver Oil; D008049:Lipase