[Gametogenesis and zygote and ookinete formation in some haemoproteid species (Haemosporidia: Haemoproteidae) of birds in vitro].
Observations were made through examination of thin blood films which were prepared at appropriate intervals from citrated fresh blood of infected birds. The comparative description of gametogenesis, zygote and ookinete formation of Haemoproteus tartakovskyi, H. belopolskyi, H. fringillae, H. pallidus under the light microscope is given for the first time. This process has a general pattern for all investigated haemoproteid species. It is shown that macrogametocyte has been transformed directly to macrogamete without forming a residual body, as it was noted by Desser (1972) for H. (= Parahaemoproteus) velans. The most informative signs, which can be used for haemoproteid systematics, were determined. Among them microgamete length, zygote structure, morphological features of developing ookinete as well as the rate of ookinete formation can be pointed out first of all. H. pallidus microgametes are twice shorter than H. tartakovskyi, H. belopolskyi, H. fringillae ones. A large clear vacuole is formed in zygote of H. fringillae only. The majority of diagnostic features has the developing ookinetes. On the basis of ookinetes morphology the investigated species of haemoproteids can be determined easily. There were clear differences between haemoproteid species on the rate of ookinete formation. The ookinetes of H. belopolskyi and H. fringillae are formed for about 12 times more slowly than H. pallidus ones, and for about 6 times more rapidly than H. tartakovskyi ones. The peculiarities of gametogenesis, zygote and ookinete formation under the stable conditions in vitro can be used as usefull additional signs for determination of haemoproteid species.
Val'kiunas
G A
GA
Ezhova
T A
TA
D000818:Animals; D001717:Birds / Q000469:parasitology*; D005718:Gametogenesis; D016787:Haemosporida / Q000145:classification / Q000254:growth & development*; D066298:In Vitro Techniques; D013045:Species Specificity; D013997:Time Factors; D015053:Zygote / Q000254:growth & development*
Gametogenez, formirovanie zigoty i ookinety u nekotorykh vidov gemoproteid (Haemosporidia: Haemoproteidae) ptits in vitro.