Growth factor modulation of surface ganglioside expression in cloned neoplastic glia.
Cloned neoplastic astrocytes from a human glioma-derived cell line (IPSB-18) were grown in fetal calf serum (FCS)-supplemented culture medium in the presence of three growth factors. Basic fibroblast growth factor (bFGF), epidermal growth factor (EGF) but not platelet-derived growth factor (PDGF) induced an increase in the number of cells positive for the ganglioside-recognizing monoclonal antibody, A2B5. No such growth factor-mediated induction could be detected in cells maintained in plasma-derived serum (PDS)-supplemented medium. Small molecules, removed from PDS during dialysis, may, therefore, act synergistically with growth factors in the control of ganglioside synthesis.
Pilkington
G J
GJ
Department of Neuropathology, Institute of Psychiatry, London, UK.
Dunan
J R
JR
Rogers
J P
JP
Clarke
T M
TM
Knott
J C
JC
D001253:Astrocytes / Q000187:drug effects / Q000378:metabolism*; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D005455:Fluorescent Antibody Technique; D005732:Gangliosides / Q000378:metabolism*; D005910:Glioma / Q000378:metabolism* / Q000473:pathology; D006651:Histocytochemistry; D006801:Humans; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D005732:Gangliosides; D010982:Platelet-Derived Growth Factor; D016222:Fibroblast Growth Factor 2; D004815:Epidermal Growth Factor