The phospholipase activities present in preheparin mouse plasma are inhibited by antiserum to hepatic lipase.
1. Preheparin plasma from mice, but not rats or man, contains high levels of phospholipase A and lysophospholipase activities which are distinct from lecithin:cholesterol acyltransferase (LCAT). 2. Neither the phospholipase A nor the lysophospholipase activities in preheparin plasma are inhibited by incubation in the presence of protamine sulphate or high salt concentrations. 3. When mouse plasma is incubated in the presence of an antiserum specific for rat hepatic triacylglycerol lipase (HTGL), the phospholipase activities are abolished. 4. These observations suggest that the phospholipase activities are attributable to the action of HTGL, which, in the mouse appears to be a freely circulating enzyme, whereas for other species this enzyme only appears in the blood following administration of heparin.
Gillett
M P
MP
Departamento de Bioquímica, Universidade Federal de Pernambuco, Recife-PE, Brazil.
Vieira
E M
EM
Dimenstein
R
R
D000818:Animals; D006493:Heparin / Q000494:pharmacology*; D006801:Humans; D007106:Immune Sera; D008049:Lipase / Q000276:immunology*; D008099:Liver / Q000201:enzymology*; D008245:Lysophospholipase / Q000037:antagonists & inhibitors* / Q000097:blood; D051379:Mice; D007862:Phosphatidylcholine-Sterol O-Acyltransferase / Q000097:blood; D010741:Phospholipases A / Q000037:antagonists & inhibitors* / Q000097:blood; D011479:Protamines / Q000494:pharmacology; D051381:Rats; D012965:Sodium Chloride / Q000494:pharmacology
D007106:Immune Sera; D011479:Protamines; D012965:Sodium Chloride; D006493:Heparin; D007862:Phosphatidylcholine-Sterol O-Acyltransferase; D008049:Lipase; D010741:Phospholipases A; D008245:Lysophospholipase