Large scale, liquid phase synthesis of oligonucleotides by the phosphoramidite approach.
A new method for the liquid phase synthesis of oligonucleotides is described which makes use of polyethylene glycol (PEG) as soluble support and phosphoramidite derivatives as synthons. The new synthetic protocol was applied to a quite large scale production (about 100 mumoles) of such compounds up to the 20mer level. This solution method, called HELP High Efficiency Liquid Phase) Plus, appears effective in terms of speed and coupling yield and can be evaluated for the production of large amount of oligonucleotides.
Bonora
G M
GM
Department of Organic Chemistry, CNR, University of Padova, Italy.
Biancotto
G
G
Maffini
M
M
Scremin
C L
CL
D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D009682:Magnetic Resonance Spectroscopy; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis*; D009943:Organophosphorus Compounds / Q000737:chemistry*; D011092:Polyethylene Glycols
D009838:Oligodeoxyribonucleotides; D009943:Organophosphorus Compounds; D011092:Polyethylene Glycols