[Metabolism of nuclear neutral lipids and rat thymocyte chromatin under normal conditions and after gamma-irradiation].
The levels and specific radioactivities of cholesterol and free fatty acids in nuclei and chromatin of thymocytes from normal and gamma-irradiated (10 Gy) rats have been studied. The radioactivity of the total lipid fraction of gamma-irradiated cells was decreased significantly in the absence of inhibition of [2-14C]acetate incorporation into the total protein and lipid reactions and the [3H]uracil incorporation into the acid-insoluble RNA. The concentration of free fatty acids in the nuclei increased significantly after irradiation. The specific radioactivity of cholesterol in chromatin was higher than in the nuclei. The differences in specific radioactivities of free fatty acids were less pronounced. After irradiation the ratio of specific radioactivities of free fatty acids in chromatin to that in the nuclei showed a tendency to increase.
Kulagina
T P
TP
Shuruta
S A
SA
Kolomiĭtseva
I K
IK
D000818:Animals; D002250:Carbon Radioisotopes; D002784:Cholesterol / Q000378:metabolism*; D002843:Chromatin / Q000378:metabolism*; D005230:Fatty Acids, Nonesterified / Q000378:metabolism*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013950:Thymus Gland / Q000166:cytology / Q000378:metabolism* / Q000528:radiation effects
D002250:Carbon Radioisotopes; D002843:Chromatin; D005230:Fatty Acids, Nonesterified; D002784:Cholesterol
Metabolizm neĭtral'nykh lipidov iader i khromatina timotsitov krys v norme i posle gamma-oblucheniia.