A method for rapid exchange of solutions at membrane patches using a 10-microliters microcapsule.
A rapid exchange (less than 2 ms) of the bath solution facing a membrane patch is accomplished by driving the tip of a pipette from the bath through a 100-microns oil layer into a small capsule filled with 10 microliters test solution. The microcapsule method can be applied to both excised patch configurations, inside-out and outside-out patches. On and off reactions of Ca(2+)-activated K+ channel activity have been recorded after changing the intracellular Ca2+ concentration using an inside-out patch. A blockade of these K+ channels by external tetraethylammonium ions is demonstrated with an outside-out patch. The blocking kinetics of delayed-rectifier K+ channels by a purified peptide toxin from snake venom, dendrotoxin, could be measured with our microcapsule method. Using tiny volumes of test solutions this method can be helpful in experiments involving scarce or expensive solutions.
Koh
D S
DS
Physiologisches Institut, Justus-Liebig-Universität, Giessen, Germany.
Vogel
W
W
D000818:Animals; D002118:Calcium / Q000494:pharmacology*; D002214:Capsules; D002462:Cell Membrane / Q000187:drug effects / Q000502:physiology*; D004546:Elapid Venoms / Q000494:pharmacology; D004553:Electric Conductivity; D004594:Electrophysiology; D007700:Kinetics; D015221:Potassium Channels / Q000187:drug effects / Q000502:physiology*; D012996:Solutions; D019789:Tetraethylammonium; D013757:Tetraethylammonium Compounds / Q000494:pharmacology; D014982:Xenopus laevis
D002214:Capsules; D004546:Elapid Venoms; D015221:Potassium Channels; D012996:Solutions; D013757:Tetraethylammonium Compounds; D019789:Tetraethylammonium; C020273:dendrotoxin; D002118:Calcium