Monoclonal antibodies prepared against heparin lyase I and their reactivity toward heparin lyase I, II and III.
1. Six different monoclonal IgG mouse antibodies to heparin lyase I from Flavobacterium heparinum were prepared. 2. The monoclonal antibodies were used to detect heparin lyases I, II and III by dot-blotting immunoassay and by Western blotting. 3. Individual antibodies showed different reactivity toward the three heparin lyases. 4. The reactivity of two of the monoclonal antibodies was destroyed by exposing heparin lyases to sodium dodecyl sulfate. 5. The antibodies can be used to rapidly distinguish between the three heparin lyases.
Gu
K
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Division of Medicinal and Natural Products Chemistry, College of Pharmacy, University of Iowa, Iowa City 52242.
Edens
R E
RE
Jandik
K A
KA
Linhardt
R J
RJ
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D015153:Blotting, Western; D005417:Flavobacterium / Q000201:enzymology; D019763:Heparin Lyase; D007118:Immunoassay; D007074:Immunoglobulin G / Q000276:immunology*; D051379:Mice; D011133:Polysaccharide-Lyases / Q000276:immunology*; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D012967:Sodium Dodecyl Sulfate; D011133:Polysaccharide-Lyases; D019763:Heparin Lyase