title: Carbonic anhydrase isoenzymes II and I are present in the zona glomerulosa cells of the human adrenal gland.
abstract: Human carbonic anhydrase isoenzymes I and II (HCA I and II) were purified from human erythrocytes by inhibitor affinity chromatography and ion-exchange chromatography. These isoenzymes were then located in the human adrenal gland using specific polyclonal antisera raised in rabbits and specific detection by immunohistochemical techniques. Both HCA II and I were located in the zona glomerulosa cells, although the staining for HCA I was faint. The cells of the zona fasciculata and the zona reticularis failed to stain with either antiserum. Control stainings with preimmune or anti-HCA VI sera were negative. The presence of HCA II and I in the zona glomerulosa cells may be linked to regulation of the biosynthesis or secretion of mineralocorticoids.
authors: University of Oulu, Department of Anatomy, Finland.
authors: A K
authors: AK
authors: Parkkila
authors: S
authors: S
authors: Parkkila
authors: T
authors: T
authors: Juvonen
authors: H
authors: H
authors: Rajaniemi
mesh_terms: D000918:Antibody Specificity; D015153:Blotting, Western; D002256:Carbonic Anhydrases / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D002852:Chromatography, Ion Exchange; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000201:enzymology; D006801:Humans; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D015384:Zona Glomerulosa / Q000166:cytology / Q000201:enzymology* / Q000276:immunology
chemical_list: D007527:Isoenzymes; D002256:Carbonic Anhydrases