Purification and crystallization of human cyclin-dependent kinase 2.
The major transitions of the eukaryotic cell cycle are triggered by cyclin-dependent protein kinases. We report the purification and crystallization of the catalytic subunit of human cyclin-dependent kinase 2 (CDK2), which has been implicated in the control of the G1/S transition. CDK2 was purified in large quantities from insect cells infected with a recombinant baculovirus, and crystals of the protein were prepared and subjected to preliminary X-ray diffraction analysis.
Rosenblatt
J
J
Department of Physiology, University of California, San Francisco 94143-0444.
De Bondt
H
H
Jancarik
J
J
Morgan
D O
DO
Kim
S H
SH
D000818:Animals; D016367:Baculoviridae / Q000235:genetics; D001483:Base Sequence; D042846:CDC2-CDC28 Kinases; D002478:Cells, Cultured; D003460:Crystallization; D051357:Cyclin-Dependent Kinase 2; D018844:Cyclin-Dependent Kinases; D006801:Humans; D008969:Molecular Sequence Data; D009036:Moths; D011494:Protein Kinases / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification*; D017346:Protein Serine-Threonine Kinases; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000302:isolation & purification
D011994:Recombinant Proteins; D011494:Protein Kinases; D017346:Protein Serine-Threonine Kinases; D042846:CDC2-CDC28 Kinases; C495894:CDK2 protein, human; D051357:Cyclin-Dependent Kinase 2; D018844:Cyclin-Dependent Kinases