Endothelin-1 is produced and secreted by neonatal rat cardiac myocytes in vitro.
Production of endothelin-1 in the cultured neonatal rat cardiac myocytes was investigated. Studies on the cultured cardiac myocytes with reverse transcription-polymerase chain reaction followed by Southern hybridization indicated that mRNA of ET-1(preproET-1 mRNA) was expressed by cardiac myocytes. ET-1 polypeptide was also detected in the cultured cardiac myocytes. Moreover, ET-1 was secreted into the culture medium by them. These results suggest that the cardiac myocytes, which have been regarded as target cells of ET-1, are able to synthesize and secrete ET-1. Furthermore, there may be a possible autocrine mechanism of ET-1 in the myocytes of a heart.
Suzuki
T
T
National Institute of Biosciences and Human-Technology, Agency of Industrial Science and Technology, Ibaraki, Japan.
Kumazaki
T
T
Mitsui
Y
Y
D000818:Animals; D000831:Animals, Newborn; D001483:Base Sequence; D016232:Endothelins / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D009206:Myocardium / Q000378:metabolism*; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar
D016232:Endothelins; D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger