Molecular cloning of the b subunit of mouse coagulation factor XIII and assignment of the gene to chromosome 1: close evolutionary relationship to complement factor H.
The b subunit of human coagulation factor XIII (FXIII-b) is composed of 10 short consensus repeats (SCRs) characteristic of the regulatory proteins of complement activation system. A full-length cDNA clone of mouse FXIII-b was isolated and the entire sequence was determined. The predicted amino acid sequence showed 77.5% homology with human FXIII-b, although mouse FXIII-b contained seven extra amino acid residues at the carboxyl terminal. The strong reactivity of the translation product of this clone with rabbit anti-human FXIII-b antiserum confirmed that it encodes a mouse counterpart of the human FXIII-b. By in situ hybridization and mapping studies using 66 interspecific backcross mice, the mouse FXIII-b gene (designated F13b) was shown to be located on distal chromosome 1 closely linked to Cfh, extending a conserved linkage group between human and mouse chromosome 1. In addition, a significant structural similarity between FXIII-b and complement factor H is described.
Nonaka
M
M
Department of Immunobiology, Kanazawa University, Japan.
Matsuda
Y
Y
Shiroishi
T
T
Moriwaki
K
K
Nonaka
M
M
Natsuume-Sakai
S
S
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005075:Biological Evolution; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D017242:Complement Factor H / Q000235:genetics*; D004247:DNA; D005176:Factor XIII / Q000235:genetics*; D008040:Genetic Linkage; D017403:In Situ Hybridization; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008969:Molecular Sequence Data; D016415:Sequence Alignment
C493495:CFH protein, human; D017242:Complement Factor H; D004247:DNA; D005176:Factor XIII