Uptake of phosphatidylserine liposomes by rat Peyer's patches following intraluminal administration.
Uptake of the nonabsorbable marker 6-carboxyfluorescein was investigated both free and encapsulated in liposomes as a function of their surface charge and hydrodynamic diameter in rat Peyer's patch and nonpatch tissue. Significant uptake of the marker occurred only when encapsulated in liposomes consisting of at least 25 mol% phosphatidylserine and was highest in Peyer's patches. 6-Carboxyfluorescein encapsulated in liposomes equal to or greater than 374 nm was preferentially taken up by Peyer's patches. There was a trend to higher uptake in lower intestinal segments. These findings were supported by fluorescence microscopic observations. Uptake by Peyer's patches was specific for negatively charged liposomes as judged from competition studies.
Tomizawa
H
H
Tokyo College of Pharmacy, Japan.
Aramaki
Y
Y
Fujii
Y
Y
Hara
T
T
Suzuki
N
N
Yachi
K
K
Kikuchi
H
H
Tsuchiya
S
S
D000704:Analysis of Variance; D000818:Animals; D005452:Fluoresceins / Q000493:pharmacokinetics*; D008081:Liposomes / Q000493:pharmacokinetics*; D008297:Male; D008351:Mannans / Q000737:chemistry; D008856:Microscopy, Fluorescence; D010581:Peyer's Patches / Q000378:metabolism*; D010718:Phosphatidylserines; D051381:Rats; D017208:Rats, Wistar
D005452:Fluoresceins; D008081:Liposomes; D008351:Mannans; D010718:Phosphatidylserines; C024098:6-carboxyfluorescein