Cytogenetic sexing of mouse embryos.
Cytogenetic sexing by means of chromosome C-banding was carried out in 1/2 and 2/4 biopsied mouse embryos. Chromosome spreads were obtained in 85.0% of 1/2-embryos and in 77.5% of 2/4-embryos. Sexing was accomplished in 30.0% of 1/2-embryos and 52.5% of 2/4-embryos, with a loss due to poor technique of 12.5 and 15.0% respectively. Transfer of sexed embryos resulted in a low implantation rate. The two live fetuses obtained were of the predicted sex.
Giménez
C
C
Dept. de Biologia Cellular i Fisiologia, Universitat Autònoma de Barcelona, Bellaterra, Spain.
Egozcue
J
J
Vidal
F
F
D000818:Animals; D001757:Blastomeres; D002871:Chromosome Banding; D004624:Embryo Transfer; D047108:Embryonic Development; D005260:Female; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D011247:Pregnancy; D012732:Sex Determination Analysis / Q000379:methods*