Nonradioactive assay for adenosine 5'-phosphosulfate sulfotransferase using reversed-phase ion-pair high-performance liquid chromatography.
A nonradioactive and simple method for the assay of adenosine 5'-phosphosulfate sulfotransferase (APSSTase) is described. The method is based upon the quantitative measurement of the substrate adenosine 5'-phosphosulfate and/or product AMP by ion-pair reversed-phase high-performance liquid chromatography on a C18 column. Isocratic elution renders the method simple and fast. This method is standardized for partially purified APSSTase from the marine red macroalga Porphyra yezoensis.
Kanno
N
N
School of Fisheries Sciences, Kitasato University, Iwate, Japan.
Nagahisa
E
E
Sato
M
M
Sato
Y
Y
D002851:Chromatography, High Pressure Liquid; D000461:Rhodophyta / Q000201:enzymology*; D015238:Sulfotransferases / Q000032:analysis*
D015238:Sulfotransferases; C081239:adenosine 5'-phosphosulfate sulfotransferase