[Molecular-genetic analysis of expression features of the evolutionary-conservative neurogene Nc73EF of Drosophila melanogaster].
Molecular-genetic analysis of the Nc (neural conserved) genome sequence of Drosophila melanogaster located at the position 73EF of Drosophila melanogaster was performed. The Nc73EF sequence was shown to be expressed in the nervous system of Drosophila. We constructed the restriction map of this sequence and revealed the main RNA-coding fragment in the 5'-3' orientation. The RNA-dot analysis data demonstrated that expression of the Nc73EF transcripts took place mainly in the nervous system. Hybridization with the human brain poly(A)+RNA confirmed the basic RNA-coding fragment to be evolutionary conservative. Southern blot analysis showed this fragment to be unique in the Drosophila genome. Northern blots detected three transcripts of this DNA fragment.
Gruntenko
N E
NE
Perelygina
L M
LM
Khlebodarova
T M
TM
Kokoza
V A
VA
D000818:Animals; D001483:Base Sequence; D005075:Biological Evolution; D017124:Conserved Sequence; D004247:DNA / Q000235:genetics; D004331:Drosophila melanogaster / Q000235:genetics*; D015870:Gene Expression; D006801:Humans; D009420:Nervous System / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000235:genetics; D015183:Restriction Mapping
D012333:RNA, Messenger; D004247:DNA
Molekuliarno-geneticheskiĭ analiz osobennosteĭ ékspressii évoliutsionno-konservativnogo neĭrogena Nc73EF Drosophila melanogaster.