Modified procedure for labelling target cells in a europium release assay of natural killer cell activity.
Lanthanide europium chelated to diethylenetriaminopentaacetate (EuDTPA) can be used to label target cells such as tumor cells and lymphocytes (Blomberg et al., 1986a,b; Granberg et al., 1988). This procedure has permitted the development of new non-radioactive methods for the detection of target cell cytolysis by natural killer (NK) cells (Blomberg et al., 1986a,b), cytotoxic T lymphocytes (CTL) (Granberg et al., 1988) or complement-mediated cytolysis (Cui et al., 1992). However, we had no success with this method because of a lack of comparability between human NK cell activity simultaneously measured by a classical 51Cr release assay (Seaman et al., 1981) and EuDTPA release assay (Blomberg et al., 1986a). Furthermore, cell division and cell viability were significantly impaired by the suggested concentrations of EuCl3. In this paper, we present a modified non-cytotoxic method for target cell labelling with EuDTPA while cells are growing in culture medium.
Pacifici
R
R
Clinical Biochemistry Department, Istituto Superiore di Sanità, Rome, Italy.
Di Carlo
S
S
Bacosi
A
A
Altieri
I
I
Pichini
S
S
Zuccaro
P
P
D003601:Cytotoxicity Tests, Immunologic / Q000379:methods*; D005063:Europium; D006801:Humans; D066298:In Vitro Techniques; D007694:Killer Cells, Natural / Q000276:immunology*; D004915:Leukemia, Erythroblastic, Acute / Q000473:pathology; D014407:Tumor Cells, Cultured
D005063:Europium