title: Detection and isolation of Mycoplasma fermentans from urine of human immunodeficiency virus type 1-infected patients.
abstract: Mycoplasma fermentans (incognitus strain) has been linked with acquired immunodeficiency syndrome-associated nephropathy. Ten (23%) of 43 urine sediment specimens from 40 human immunodeficiency virus (HIV)-positive patients at different stages of acquired immunodeficiency syndrome disease tested positive in the polymerase chain reaction using a primer pair found in the insertion sequences specific to M fermentans. Mycoplasma fermentans was isolated from two HIV-positive patients' urine sediment specimens and on a repeated basis from one. All three culture-positive urine sediment specimens tested positive in the polymerase chain reaction. Fifty urine sediment specimens from age-matched HIV-negative healthy controls tested negative for M fermentans by polymerase chain reaction. Mycoplasma fermentans was not isolated from any of the control urine sediment specimens. Our results show a high prevalence of M fermentans in urine sediment specimens from HIV-positive patients but not from urine sediment specimens of HIV-negative healthy controls.
authors: Department of Infectious and Parasitic Diseases Pathology, Armed Forces Institute of Pathology, Washington, DC 20306-6000.
authors: M S
authors: MS
authors: Dawson
authors: M M
authors: MM
authors: Hayes
authors: R Y
authors: RY
authors: Wang
authors: D
authors: D
authors: Armstrong
authors: R B
authors: RB
authors: Kundsin
authors: S C
authors: SC
authors: Lo
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000150:complications / Q000652:urine*; D015139:Blotting, Southern; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D006801:Humans; D009175:Mycoplasma Infections / Q000150:complications / Q000652:urine*; D016858:Mycoplasma fermentans / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction
chemical_list: D004269:DNA, Bacterial