Cellular localization of angiotensin type 1 receptor and angiotensinogen mRNAs in the subfornical organ of the rat brain.
The cellular localization of angiotensin type 1 receptor (AT 1) and angiotensinogen mRNA expression in the subfornical organ (SFO) of the rat brain has been studied by means of non-radioactive in situ hybridization combined with immunocytochemistry for glial fibrillary acidic protein (GFAP) and Neutral red staining. The AT 1 receptor mRNA expression is shown to be within putative nerve cells without any association with the glial fibrillary acidic protein (GFAP)-immunoreactive (IR) cells. In contrast the angiotensinogen cRNA expression is associated predominantly with GFAP-IR cells. The results demonstrate that a neuronal AT 1 receptor mediates the actions of circulating angiotensin II on the SFO and that the angiotensinogen mRNA is predominantly expressed in the SFO astroglial cells.
Lippoldt
A
A
Max-Delbrück-Center for Molecular Medicine [MDC], Berlin-Buch FRG.
Bunnemann
B
B
Iwai
N
N
Metzger
R
R
Inagami
T
T
Fuxe
K
K
Ganten
D
D
D000803:Angiotensin I / Q000378:metabolism*; D000808:Angiotensinogen / Q000096:biosynthesis*; D000818:Animals; D001253:Astrocytes / Q000378:metabolism; D001921:Brain / Q000378:metabolism*; D002467:Cell Nucleus / Q000648:ultrastructure; D005904:Glial Fibrillary Acidic Protein / Q000276:immunology / Q000378:metabolism; D007124:Immunoenzyme Techniques; D017403:In Situ Hybridization; D008297:Male; D009499:Neutral Red; D015347:RNA Probes; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011945:Receptors, Angiotensin / Q000187:drug effects / Q000378:metabolism*; D013356:Subfornical Organ / Q000378:metabolism*
D005904:Glial Fibrillary Acidic Protein; D015347:RNA Probes; D012333:RNA, Messenger; D011945:Receptors, Angiotensin; D000808:Angiotensinogen; D009499:Neutral Red; D000803:Angiotensin I