Using fusions with luxAB from Vibrio harveyi MAV to quantify induction and catabolite repression of the xyl operon in Staphylococcus carnosus TM300.
The luxA,B genes from the Gram-negative marine bacterium Vibrio harveyi MAV were used in Staphylococcus carnosus TM300 as a reporter system for regulated expression of xylose utilization. The luciferase genes were fused to the xyl operon from Staphylococcus xylosus C2a. Expression of bioluminescence was induced through addition of xylose and repressed in the presence of glucose. A method to quantitate bioluminescence directly from the culture is described.
Sizemore
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Lehrstuhl für Mikrobiologie, Institut für Mikrobiologie und Biochemie der Friedrich-Alexander Universität Erlangen-Nürnberg, FRG.
Geissdörfer
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Hillen
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D001426:Bacterial Proteins / Q000235:genetics; D003001:Cloning, Molecular; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D008156:Luciferases / Q000235:genetics*; D009876:Operon; D013210:Staphylococcus / Q000235:genetics* / Q000378:metabolism; D014733:Vibrio / Q000235:genetics* / Q000378:metabolism; D014994:Xylose / Q000378:metabolism
D001426:Bacterial Proteins; D014994:Xylose; D008156:Luciferases