Analysis of synthetic oligodeoxyribonucleotides by capillary gel electrophoresis and anion-exchange HPLC.
Polyacrylamide slab gel electrophoresis has been traditionally used for the analysis of synthetic oligodeoxyribonucleotides. As the demand for oligonucleotides of high purity has increased, a need for additional high resolution analytical techniques has arisen. This report describes the principles and application of capillary gel electrophoresis and high-performance liquid chromatography for the analysis of synthetic DNA varying in base composition and length. From the results of a series of comparative experiments, the relative advantages and disadvantages of each technique are discussed.
Warren
W J
WJ
Millipore Corporation, Waters Chromatography Division, Millford, MA 01757.
Vella
G
G
D001483:Base Sequence; D001709:Biotechnology; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000706:statistics & numerical data; D002852:Chromatography, Ion Exchange / Q000379:methods* / Q000706:statistics & numerical data; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods* / Q000706:statistics & numerical data; D005069:Evaluation Studies as Topic; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000032:analysis* / Q000138:chemical synthesis / Q000737:chemistry / Q000592:standards; D012015:Reference Standards; D015203:Reproducibility of Results
D009838:Oligodeoxyribonucleotides; C027903:oligo (dT); C030570:oligodeoxyadenylic acid