Direct high-performance liquid chromatographic determination in urine of the enantiomers of propranolol and its major basic metabolite 4-hydroxypropranolol.
A method is described for quantitation of underivatized enantiomers of propranolol and its major basic metabolite, 4-hydroxypropranolol, in urine samples by high-performance liquid chromatography with fluorescence detection, using a cellulose tris(3,5-dimethylphenylcarbamate) chiral stationary phase. This method was found to be precise and accurate for the measurement of propranolol and 4-hydroxypropranolol concentrations in urine from pharmacokinetic investigations. This method represents the first assay for direct determination of 4-hydroxypropranolol enantiomers. The ability to easily measure 4-hydroxypropranolol enantiomers is valuable because the stereoselective disposition of propranolol is primarily due to stereoselective metabolism in the pathway responsible for generation of 4-hydroxypropranolol.
Herring
V L
VL
Department of Clinical Pharmacy, College of Pharmacy, University of Tennessee, Memphis 38163.
Johnson
J A
JA
D002851:Chromatography, High Pressure Liquid; D005965:Glucuronates / Q000652:urine; D006801:Humans; D008297:Male; D010084:Oxidation-Reduction; D011433:Propranolol / Q000031:analogs & derivatives* / Q000652:urine; D013050:Spectrometry, Fluorescence; D013237:Stereoisomerism
D005965:Glucuronates; C001422:4-hydroxypropranolol; D011433:Propranolol