title: A recombination-based assay demonstrates that the fragile X sequence is transcribed widely during development.
abstract: To identify transcribed sequences rapidly and efficiently, we have developed a recombination-based assay to screen bacteriophage lambda libraries for sequences that share homology with a given probe. This strategy determines analytically whether a given probe is transcribed in a given tissue at a given time of development, and may also be used to isolate preparatively the transcribed sequence free of the screening probe. We illustrate this technology for the fragile X sequence, demonstrating that it is transcribed ubiquitously in an 11 week fetus, in a variety of 20 week human fetal tissues, including brain, spinal cord, eye, liver, kidney and skeletal muscle, and in adult jejunum.
authors: University of Michigan Medical School, Howard Hughes Medical Institute, Ann Arbor 48109-0650.
authors: A J
authors: AJ
authors: Hanzlik
authors: M M
authors: MM
authors: Osemlak-Hanzlik
authors: M A
authors: MA
authors: Hauser
authors: D M
authors: DM
authors: Kurnit
mesh_terms: D000328:Adult; D010582:Bacteriophage lambda / Q000235:genetics; D001483:Base Sequence; D004247:DNA; D015342:DNA Probes; D005333:Fetus / Q000378:metabolism*; D051860:Fragile X Messenger Ribonucleoprotein 1; D005600:Fragile X Syndrome / Q000196:embryology / Q000235:genetics* / Q000378:metabolism; D005821:Genetic Techniques; D006801:Humans; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000032:analysis* / Q000235:genetics; D009928:Organ Specificity / Q000235:genetics; D016133:Polymerase Chain Reaction; D016601:RNA-Binding Proteins; D011995:Recombination, Genetic; D014158:Transcription, Genetic
chemical_list: D004247:DNA; D015342:DNA Probes; D051860:Fragile X Messenger Ribonucleoprotein 1; D009419:Nerve Tissue Proteins; D016601:RNA-Binding Proteins; C497683:FMR1 protein, human