title: Overproduction of proteins using expression-cassette polymerase chain reaction.
authors: Department of Chemistry, Harvard University, Cambridge, Massachusetts 02138.
authors: K D
authors: KD
authors: MacFerrin
authors: L
authors: L
authors: Chen
authors: M P
authors: MP
authors: Terranova
authors: S L
authors: SL
authors: Schreiber
authors: G L
authors: GL
authors: Verdine
mesh_terms: D001483:Base Sequence; D015704:CD4 Antigens / Q000096:biosynthesis* / Q000235:genetics*; D002460:Cell Line; D003062:Codon / Q000235:genetics; D004274:DNA, Recombinant / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics*; D015870:Gene Expression; D006801:Humans; D007202:Indicators and Reagents; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011401:Promoter Regions, Genetic; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics*; D015183:Restriction Mapping; D013698:Templates, Genetic; D013728:Terminator Regions, Genetic; D014158:Transcription, Genetic
chemical_list: D015704:CD4 Antigens; D003062:Codon; D004274:DNA, Recombinant; D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides; D011506:Proteins