One-step competitive immunochromatographic assay for semiquantitative determination of lipoprotein(a) in plasma.
Numerous studies have associated high concentrations of lipoprotein(a) [Lp(a)] with atherosclerosis. We developed a rapid, one-step competitive immunochromatographic assay to measure Lp(a) in plasma. The assay is performed on a nitrocellulose membrane strip and the result is determined by a visual readout of rust-colored colloidal selenium. The assay is based on the principle that Lp(a) in the sample will compete with Lp(a)-coated colloidal selenium for binding to the anti-Lp(a) monoclonal antibody immobilized on the assay strip in the format of four ladder bars. The number of capture bars that appear as a result of the formation of colloidal selenium color is proportional to the concentration of the Lp(a) protein in the samples. The strip assay semiquantitatively measures Lp(a) concentrations ranging from 0 to 180 mg/L of Lp(a) protein in serum, plasma, or fingerstick whole-blood samples. This assay appears very useful for quick identification of individuals with above-normal concentrations of plasma Lp(a) protein (> 70 mg/L), and has potential for monitoring a patient's response to treatment with Lp(a)-lowering drugs.
Lou
S C
SC
Abbott Diagnostics Division, Abbott Laboratories, North Chicago, IL 60064-3500.
Patel
C
C
Ching
S
S
Gordon
J
J
D000818:Animals; D000911:Antibodies, Monoclonal; D001667:Binding, Competitive; D002845:Chromatography / Q000379:methods*; D003101:Collodion; D003102:Colloids; D005260:Female; D006801:Humans; D007118:Immunoassay; D017270:Lipoprotein(a) / Q000097:blood*; D051379:Mice; D008807:Mice, Inbred BALB C; D011786:Quality Control; D011934:Reagent Strips; D012643:Selenium
D000911:Antibodies, Monoclonal; D003102:Colloids; D017270:Lipoprotein(a); D011934:Reagent Strips; D003101:Collodion; D012643:Selenium