title: Isolation and characterization of simian immunodeficiency virus from African white-crowned mangabey monkeys (Cercocebus torquatus lunulatus).
abstract: Forty-eight of 236 sera from seven species of African non-human primates in Kenya, including those of white-crowned mangabey monkeys (Cercocebus torquatus lunulatus) had antibodies to simian immunodeficiency viruses (SIVs). Isolates of simian lentivirus were obtained from seropositive white-crowned mangabey monkeys which are indigenous in West Africa. This virus, designated as SIVWCM, appeared morphologically similar to HIV by electron microscopy, showed Mg(2+)-dependent reverse transcriptase activity, and induced cytopathic effects in human CD4-positive cells. Western blotting analysis revealed that env products of SIVWCM cross-reacted with those of SIVAGM more strongly than with those of HIV-1 and SIVMAC, and clear hybridization bands were detected with an SIVAGM probe. For comparison of the virus sequence with those of other primate lentiviruses, part of the pol gene and the long terminal repeats (LTRs) were amplified and cloned. Sequencing showed that SIVWCM isolates were closely related to SIVAGM isolates. This study suggested that SIVAGM from the Cercopithecus genus and SIVWCM from the Cercocebus genus may be members of an SIV group that is genetically distinct from the SIV from a sooty mangabey monkey (SIVSMM) of the genus Cercocebus, to which the white-crowned mangabey monkey also belongs.
authors: Research Center for Immunodeficiency Virus, Kyoto University, Japan.
authors: K
authors: K
authors: Tomonaga
authors: J
authors: J
authors: Katahira
authors: M
authors: M
authors: Fukasawa
authors: M A
authors: MA
authors: Hassan
authors: M
authors: M
authors: Kawamura
authors: H
authors: H
authors: Akari
authors: T
authors: T
authors: Miura
authors: T
authors: T
authors: Goto
authors: M
authors: M
authors: Nakai
authors: M
authors: M
authors: Suleman
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D001483:Base Sequence; D015139:Blotting, Southern; D015153:Blotting, Western; D002460:Cell Line; D016664:Cercocebus / Q000382:microbiology*; D004279:DNA, Viral; D005260:Female; D015751:Genes, pol; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D012091:Repetitive Sequences, Nucleic Acid; D015302:Simian Immunodeficiency Virus / Q000145:classification / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*
chemical_list: D000914:Antibodies, Viral; D004279:DNA, Viral