A rapid spectrophotometric estimation of individual phosphates and phosphonates: its application to chromatography of sugar phosphates and their phosphonate analogues.
A compound containing the -PO3H2 group (phosphoric acid, one of its monoesters, or an alkylphosphonic acid) may be rapidly assayed by the decrease it produces in the absorbance at 450 nm of a buffered acidic solution of Fe3+ and N(-3) [corrected]. The method has been used to follow chromatograms of sugar phosphates and their phosphonomethyl analogues.
Ben-Yoseph
O
O
Department of Biochemistry, Cambridge, United Kingdom.
Sparkes
M J
MJ
Dixon
H B
HB
D002852:Chromatography, Ion Exchange; D005069:Evaluation Studies as Topic; D019298:Glucose-6-Phosphate; D005958:Glucosephosphates / Q000032:analysis; D063065:Organophosphonates / Q000032:analysis*; D009943:Organophosphorus Compounds / Q000032:analysis; D013053:Spectrophotometry / Q000379:methods*; D013403:Sugar Phosphates / Q000032:analysis*
D005958:Glucosephosphates; D063065:Organophosphonates; D009943:Organophosphorus Compounds; D013403:Sugar Phosphates; C032627:methylphosphonic acid; D019298:Glucose-6-Phosphate