Cell-free expression of large collections of cDNA clones using positive-selection lambda phage vectors.
Coleclough
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Department of Immunology, St. Jude Children's Research Hospital, Memphis, Tennessee 38101.
D000818:Animals; D010582:Bacteriophage lambda / Q000235:genetics*; D001483:Base Sequence; D002474:Cell-Free System; D003001:Cloning, Molecular / Q000379:methods*; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D004274:DNA, Recombinant / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D015723:Gene Library; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D009838:Oligodeoxyribonucleotides; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger / Q000235:genetics* / Q000302:isolation & purification; D011817:Rabbits; D015183:Restriction Mapping; D012156:Reticulocytes / Q000378:metabolism; D012641:Selection, Genetic; D014158:Transcription, Genetic
D004274:DNA, Recombinant; D009838:Oligodeoxyribonucleotides; D011506:Proteins; D012333:RNA, Messenger; D004247:DNA