[Simulating and inhibiting effect of individual blood serum transport proteins on thyroid hormone binding with human placental cell membrane].
Binding processes in in vitro systems modelling specific interactions between transport and receptor proteins, thyroid hormones of human placental tissue and the washing blood, have been studied. These systems included syncytiotrophoblast villous membranes, thyroxine (T4), triiodothyronine (T3) and iodothyronine-binding proteins: transthyretin, albumin, immunoglobulins (Ig) M and G, apolipoprotein A-I, and the T4-binding globulin purified from human retroplacental serum. All of the transport proteins at concentrations close to the Ka values of their complexes with thyroid hormones produced inhibitory effects on the binding of [125I]T3 or [125I]T4 to the thyroid hormone membrane receptor. The dependence of [125I]T4 membrane binding on IgM concentration in the system was characteristic of all proteins studied. In the case of T3 such dependence was unique for IgM, and included the phase of the IgM stimulatory effect (10(-11)-10(-9) M) and the phase of inhibition (10(-8)-10(-7) M). In the presence of 30 pM IgM, the concentration of the membrane T3-binding sites increased by 75% with a 2.2-fold decrease of the association constant (Ka). It was shown that IgM interacts specifically with two classes of binding sites on the plasma membranes with Ka(1) = 5.0 x 10(9) M-1, Bmax(1) = 34 fmol/mg of membrane protein and Ka(2) = 2.7 x 10(7) M-1, Bmax(2) = 2.0 pmol/mg of membrane protein. It is suggested that the stimulatory effect of IgM is caused by increases in the number of T3-binding sites on the placental villous membranes as a result of complex formation between IgM and its membrane receptor which displays an enhanced T3-binding activity.
Karpyza
E I
EI
Kiklevich
I E
IE
Ermolenko
M N
MN
Sviridov
O V
OV
D001665:Binding Sites; D001798:Blood Proteins / Q000502:physiology*; D002462:Cell Membrane / Q000378:metabolism; D005260:Female; D006801:Humans; D008565:Membrane Proteins / Q000378:metabolism; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D013963:Thyroid Hormones / Q000378:metabolism*
D001798:Blood Proteins; D008565:Membrane Proteins; D013963:Thyroid Hormones
Stimuliruiushchee i ingibiruiushchee deĭstvie individual'nykh transportnykh belkov syvorotki krovi na sviazyvanie tireoidnykh gormonov s plazmaticheskimi membranami platsenty cheloveka.