Stabilisation of a yeast artificial chromosome containing plant DNA using a recombination-deficient host.
The large genomes of many plant species contain numerous dispersed repeat sequences. The problem of large-scale structural instability due to the presence of such repeats in yeast artificial chromosome (YAC) clones was assessed. The feasibility of stabilising plant sequences prone to rearrangement in YACs was demonstrated using a host yeast strain deficient in recombination.
Dunford
R
R
Cambridge Laboratory, John Innes Centre, Norwich, UK.
Vilageliu
L
L
Moore
G
G
D015825:Chromosomes, Fungal; D015723:Gene Library; D016678:Genome; D001467:Hordeum / Q000235:genetics*; D011995:Recombination, Genetic; D012091:Repetitive Sequences, Nucleic Acid