Isolation of Antho-RFamide related peptides from the eyestalks of blue crab.
1. Two carboxyl-terminally amidated peptides (CP1 and CP2) were isolated from the blue crab (Callinectes sapidus) eyestalks by a method of carboxyl-terminal analysis. 2. The peptides were sequenced as pGlu-Gly-Arg-Phe-amide (CP1) and pGlu-Leu-Gly-Arg-Phe-amide (CP2). 3. Each carboxyl-terminus of the peptides was precisely determined by amino acid analysis utilizing phenylisothiocyanate derivatives. 4. CP1 was identical to the sea anemone neuropeptide, Antho-RFamide.
Yasuda
A
A
Suntory Institute for Bioorganic Research, Osaka, Japan.
Naya
Y
Y
Nakanishi
K
K
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D003386:Brachyura; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D005123:Eye / Q000737:chemistry*; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000737:chemistry / Q000302:isolation & purification*; D011761:Pyrrolidonecarboxylic Acid / Q000031:analogs & derivatives; D017386:Sequence Homology, Amino Acid
D000596:Amino Acids; D009479:Neuropeptides; C060288:4-pyroglutamyl-glycyl-arginyl-phenylalaninamide; C080537:pyroglutamyl-leucyl-glycyl-arginyl-phenylalaninamide; D011761:Pyrrolidonecarboxylic Acid