title: Equine protozoal myeloencephalitis: antigen analysis of cultured Sarcocystis neurona merozoites.
abstract: Antigens of cultured Sarcocystis neurona merozoites were examined using immunoblot analysis. Blotted proteins were probed with S. cruzi, S. muris, and S. neurona antisera produced in rabbits, S. fayeri (pre- and post-infection) and S. neurona (pre- and post-inoculation) sera produced in horses, immune sera from 7 histologically confirmed cases of equine protozoal myeloencephalitis (EPM), and pre-suckle serum from a newborn foal. Eight proteins, 70, 24, 23.5, 22.5, 13, 11, 10.5, and 10 Kd, were detected only by S. neurona antiserum and/or immune serum from EPM-affected horses. Equine sera were titered by the indirect immunofluorescent antibody (IFA) method using air-dried, cultured S. neurona merozoites. Anti-Sarcocystis IFA titers were found in horses with or without EPM. Serum titers did not correspond to the number of specific bands recognized on immunoblots.
authors: Department of Veterinary Science, University of Kentucky, Lexington 40546-0099.
authors: D E
authors: DE
authors: Granstrom
authors: J P
authors: JP
authors: Dubey
authors: S W
authors: SW
authors: Davis
authors: R
authors: R
authors: Fayer
authors: J C
authors: JC
authors: Fox
authors: K B
authors: KB
authors: Poonacha
authors: R C
authors: RC
authors: Giles
authors: P F
authors: PF
authors: Comer
mesh_terms: D000818:Animals; D000953:Antigens, Protozoan / Q000032:analysis* / Q000302:isolation & purification; D002417:Cattle; D002478:Cells, Cultured; D004591:Electrophoresis, Polyacrylamide Gel; D006734:Horse Diseases; D006736:Horses; D015151:Immunoblotting; D008970:Molecular Weight; D012522:Sarcocystis / Q000276:immunology* / Q000302:isolation & purification; D012523:Sarcocystosis / Q000662:veterinary*
chemical_list: D000953:Antigens, Protozoan