Generation of monoclonal antibodies against HILDA/LIF and their use in the quantitative assay of the cytokine.
An adoptive transfer immunization/fusion protocol in mice has been successfully used to raise a series of monoclonal antibodies directed against the Human Interleukin for DA-1a (HILDA)/Leukemia Inhibitory Factor (LIF) cytokine. These antibodies which were raised using recombinant HILDA/LIF purified from conditioned medium of transfected Chinese Hamster Ovary (CHO) cells also react with natural HILDA/LIF from the HSB2 T lymphoma cell line and unglycosylated HILDA/LIF produced in E. coli. They define four separate epitopes, one of which is involved in receptor binding and induction of biological activity. A sensitive sandwich immunoradiometric assay which is linear up to 5 ng/ml HILDA/LIF and can detect as low as 25 pg/ml of the cytokine has been developed.
Godard
A
A
INSERM U.211 (Unité de Recherche sur les Effecteurs Lymphocytaires T), C.H.U. Nantes, France.
Fauchet
F
F
Raher
S
S
Jadoul
M
M
Thuillier
B
B
Dehart
J
J
Soulillou
J P
JP
Baudrihaye
M
M
Jacques
Y
Y
De Groote
D
D
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D016466:CHO Cells; D002455:Cell Division / Q000502:physiology; D006224:Cricetinae; D006131:Growth Inhibitors / Q000032:analysis / Q000276:immunology* / Q000378:metabolism; D007132:Immunoglobulin Isotypes; D015592:Immunoradiometric Assay; D015850:Interleukin-6; D053605:Leukemia Inhibitory Factor; D053680:Leukemia Inhibitory Factor Receptor alpha Subunit; D008222:Lymphokines / Q000032:analysis / Q000276:immunology* / Q000378:metabolism; D011233:Precipitin Tests; D018121:Receptors, Cytokine; D011971:Receptors, Immunologic / Q000378:metabolism; D053606:Receptors, OSM-LIF
D000911:Antibodies, Monoclonal; D006131:Growth Inhibitors; D007132:Immunoglobulin Isotypes; D015850:Interleukin-6; C507837:LIF protein, human; C507840:LIFR protein, human; D053605:Leukemia Inhibitory Factor; D053680:Leukemia Inhibitory Factor Receptor alpha Subunit; D008222:Lymphokines; D018121:Receptors, Cytokine; D011971:Receptors, Immunologic; D053606:Receptors, OSM-LIF