Platelet concentrates in an additive solution prepared from pooled buffy coats. In vivo studies.
Leukocyte-depleted platelet concentrates were prepared from pools of 4 buffy coats on the day after blood collection (BC-PC). The storage medium was composed of citrate phosphate dextrose plasma and a platelet-additive solution. Autologous transfusions of 111In-labelled platelets in 9 healthy volunteers were performed on the day of preparation (day 1) and on day 5. The recovery was 54.6 +/- 8.7 (day 1) and 51.9 +/- 10.4% (day 5), T1/2 was 101 +/- 28 and 61 +/- 9 h, respectively. The survival was 8.3 +/- 1.7 and 5.7 +/- 1.0 days, respectively, using linear plot, and 7.8 +/- 2.0 and 5.8 +/- 0.5 days using the multiple hit method. In a prospective clinical study a comparison of the corrected posttransfusion increments was made between BC-PCs and apheresis-PC, and between fresh (1-2 days) and stored (3-5 days) preparations. No difference was found between BC-PCs and apheresis PCs. However, fresh BC-PCs gave higher increments than stored BC-PCs. A slight numerical difference between fresh and stored apheresis-PCs was not statistically significant. It is concluded that the BC-PC method results in platelets of equal quality to apheresis-PC.
Eriksson
L
L
Department of Clinical Immunology and Transfusion Medicine, University Hospital, Uppsala, Sweden.
Shanwell
A
A
Gulliksson
H
H
Högman
C F
CF
Svensson
L A
LA
Kristensen
J
J
Berg
B
B
D000328:Adult; D016913:Blood Component Transfusion; D001792:Blood Platelets; D001793:Blood Preservation / Q000379:methods*; D002470:Cell Survival / Q000502:physiology; D005260:Female; D006801:Humans; D007958:Leukocyte Count; D008297:Male; D008875:Middle Aged; D010976:Platelet Count; D012016:Reference Values; D012996:Solutions; D013921:Thrombocytopenia / Q000628:therapy
D012996:Solutions