title: Localization of 14 functional J regions in the human T-cell receptor alpha-chain locus.
abstract: Nearly 60 unique J alpha regions have been identified in human T-cell receptor (TCR) alpha chains to date, yet fewer than one-third of these have been localized within the alpha-chain locus. We report a rapid method for the mapping of productively rearranged J alpha regions using oligonucleotide probes and overlapping phage clones spanning 80 kb of the TCR-alpha locus. Fourteen J alpha regions were mapped using this technique; the positions of five of these have been verified by genomic cloning.
authors: VA Medical Center, San Francisco, California 94121.
authors: R C
authors: RC
authors: Harvey
authors: L C
authors: LC
authors: Showe
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D015342:DNA Probes; D006801:Humans; D008969:Molecular Sequence Data; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics*; D015183:Restriction Mapping
chemical_list: D015342:DNA Probes; D016693:Receptors, Antigen, T-Cell, alpha-beta; D004247:DNA