Pentoxifylline inhibits the expression of tissue factor mRNA in endotoxin-activated human monocytes.
Tissue factor (TF) is a transmembrane glycoprotein which, in association with factor VII(a), is the main activator of coagulation. In illnesses such as Gram-negative endotoxemia, circulating monocytes synthesize and express substantial TF activity, resulting in extensive disseminated intravascular coagulation. We investigated the way in which TF is suppressed by pentoxifylline (PTX), and found that PTX down-regulates immunologic TF expression and specific mRNA production in response to LPS. Since TF mRNA stability is not altered, this effect appears to take place at the transcriptional level.
Ollivier
V
V
INSERM U294, Paris, France.
Ternisien
C
C
Vu
T
T
Hakim
J
J
de Prost
D
D
D004731:Endotoxins / Q000494:pharmacology*; D005434:Flow Cytometry; D006801:Humans; D007700:Kinetics; D009000:Monocytes / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D010431:Pentoxifylline / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism; D013925:Thromboplastin / Q000235:genetics / Q000378:metabolism*
D004731:Endotoxins; D012333:RNA, Messenger; D013925:Thromboplastin; D010431:Pentoxifylline