Molecular characterization of the enniatin synthetase gene encoding a multifunctional enzyme catalysing N-methyldepsipeptide formation in Fusarium scirpi.
The gene encoding the multifunctional enzyme enniatin synthetase from Fusarium scirpi (esyn1) was isolated and characterized by transcriptional mapping and expression studies in Escherichia coli. This is the first example of a gene encoding an N-methyl peptide synthetase. The nucleotide sequence revealed an open reading frame of 9393 bp encoding a protein of 3131 amino acids (M(r) 346,900). Two domains designated EA and EB within the protein were identified which share similarity to each other and to microbial peptide synthetase domains. In contrast to the N-terminal domain EA, the carboxyl terminal domain EB is interrupted by a 434-amino-acid portion which shows local similarity to a motif apparently conserved within adenine and cytosine RNA and DNA methyltransferases and therefore seems to harbour the N-methyl-transferase function of the multienzyme.
Haese
A
A
Institut für Biochemie und Molekulare Biologie, Technische Universität Berlin, Germany.
Schubert
M
M
Herrmann
M
M
Zocher
R
R
D000595:Amino Acid Sequence; D016384:Consensus Sequence; D004926:Escherichia coli / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D005670:Fusarium / Q000235:genetics* / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D008745:Methylation; D008780:Methyltransferases / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010452:Peptide Biosynthesis; D010453:Peptide Synthases / Q000235:genetics*; D010455:Peptides; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic
D005656:Fungal Proteins; D010455:Peptides; D011993:Recombinant Fusion Proteins; C080947:N-methyldepsipeptide; D008780:Methyltransferases; D010453:Peptide Synthases; C095368:alpha-aminoadipyl-cysteinyl-valine synthetase; C023122:enniatin B synthetase