[Angiotensin II stimulates endothelin-1 release from human endothelial cells].
Endothelin-1 (ET-1) is an endothelium-derived vasoconstrictor peptide isolated from the culture supernatant of porcine aortic endothelial cells. This 21 amino-acid residue peptide has potent vasoconstrictive properties in vitro and in vivo. ET-1 action involves phosphatidylinositol turnover, calcium mobilization and protein kinase C activation. Endothelial cells have distinct receptors for different operating through hydrosoluble hormones. The aim of this study was to investigate on a possible role of angiotensin II (ANG II) to modulate the release ET-1 from human endothelial cells in vitro. These data revealed a time- and a dose-dependent increase of ET-1 production in response to ANG II. This mechanism may have important pathophysiological implications in vivo. In fact, a double-mechanism of secretion of ET-1 from endothelial cells could exist: one active in a physiological condition and an other in response to a vasoconstrictor stimuli (as well as ANG II). Furthermore, these results may suggest an additional favourable effect of ACE-inhibition in human hypertension therapy.
Ciafré
S A
SA
Dipartimento di Medicina Sperimentale, II Università, Roma.
D'Armiento
F P
FP
Di Gregorio
F
F
Colasanti
P
P
Di Benedetto
A
A
Langella
A
A
Di Ieso
N
N
Liguori
A
A
Colasanti
R
R
Napoli
C
C
D000804:Angiotensin II / Q000493:pharmacokinetics / Q000494:pharmacology*; D002478:Cells, Cultured / Q000187:drug effects / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D016232:Endothelins / Q000187:drug effects* / Q000378:metabolism; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D007457:Iodine Radioisotopes; D011945:Receptors, Angiotensin / Q000378:metabolism; D013268:Stimulation, Chemical; D013997:Time Factors
D016232:Endothelins; D007457:Iodine Radioisotopes; D011945:Receptors, Angiotensin; D000804:Angiotensin II
L'angiotensina II stimola il rilascio di endotelina-1 da cellule endoteliali umane.