Effects of kampo (Japanese herbal) medicine "sho-saiko-to" on DNA-synthesizing enzyme activity in 1,2-dimethylhydrazine-induced colonic carcinomas in rats.
Sho-Saiko-To (SST) is a modified Japanese traditional Chinese herbal medicine containing seven medical plants: Bupleuri radix, Pinelliae tuber, Suxtallariae radix, Zizyphi fructus, Ginseng radix, Glycyrrhizae radix, and Zingiberis recens rhizoma. This preparation has been used in the treatment of some inflammatory diseases of the respiratory system and chronic hepatitis. In the present study, the effects of SST were investigated on the activities of DNA-synthesizing enzymes in 1,2-dimethylhydrazine (DMH)-induced colonic carcinomas in rats. Six-week administration of SST prevented nearly 100% of the body weight loss and the final number of the colonic carcinomas compared to those in the rats treated with DMH alone, and suppressed the enhanced activities of thymidylate synthetase (TS) and thymidine kinase (TK) which were involved in the de novo and salvage pathways of pyrimidine synthesis, respectively, in DMH-induced colonic carcinomas. These results indicate that SST may show directly and/or indirectly inhibitory effects on the development of colonic carcinomas.
Sakamoto
S
S
Medical Research Institute, Tokyo Medical and Dental University, Japan.
Mori
T
T
Sawaki
K
K
Kawachi
Y
Y
Kuwa
K
K
Kudo
H
H
Suzuki
S
S
Sugiura
Y
Y
Kasahara
N
N
Nagasawa
H
H
D019813:1,2-Dimethylhydrazine; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D003110:Colonic Neoplasms / Q000139:chemically induced / Q000188:drug therapy* / Q000201:enzymology / Q000235:genetics; D004247:DNA / Q000096:biosynthesis*; D004127:Dimethylhydrazines; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D008297:Male; D051381:Rats; D013937:Thymidine Kinase / Q000037:antagonists & inhibitors*; D013940:Thymidylate Synthase / Q000037:antagonists & inhibitors*
D000972:Antineoplastic Agents, Phytogenic; D004127:Dimethylhydrazines; D004365:Drugs, Chinese Herbal; D004247:DNA; D013940:Thymidylate Synthase; D013937:Thymidine Kinase; D019813:1,2-Dimethylhydrazine