title: Presence of growth hormone receptors in carp liver and intestine.
abstract: Membranes were prepared from tissues of the carp Ctenopharynogodon idellus including liver, kidney, intestine, adipose tissue, ovary, gill, heart, muscle and spleen. The carp liver and intestine membranes bound 125I-labeled bovine growth hormone and the binding could be displaced by cold bovine growth hormone. No changes in the ability to bind 125I-labeled bovine growth hormone occurred after treatment of the carp liver membrane with DNase, RNase, alpha-amylase and beta-glucosidase, suggesting that neither nucleic acids nor carbohydrates played an important part in the hepatic binding of growth hormone. Treatment of carp liver membranes with either chymotrypsin or trypsin produced a decrease in the growth hormone binding activity, indicating that the growth hormone receptor on carp liver membrane is a protein. Treatment of carp liver membranes with p-chloromercuribenzoate brought about a reduction in 125I-bGH binding. The inhibition could be reversed by dithioerythritol, suggesting the involvement of essential sulfhydryl group in bGH binding.
authors: Department of Biochemistry, Chinese University of Hong Kong, N.T.
authors: T B
authors: TB
authors: Ng
authors: L
authors: L
authors: Kwan
authors: C H
authors: CH
authors: Cheng
mesh_terms: D000818:Animals; D001665:Binding Sites; D002347:Carps / Q000378:metabolism*; D002729:Chloromercuribenzoates / Q000494:pharmacology; D004798:Enzymes / Q000378:metabolism; D005260:Female; D013006:Growth Hormone / Q000378:metabolism; D007422:Intestines / Q000737:chemistry*; D008099:Liver / Q000737:chemistry*; D011986:Receptors, Somatotropin / Q000032:analysis* / Q000378:metabolism; D014018:Tissue Distribution
chemical_list: D002729:Chloromercuribenzoates; D004798:Enzymes; D011986:Receptors, Somatotropin; D013006:Growth Hormone