Localization of seven new genes around the HLA-A locus.
A yeast artificial chromosome (YAC B30) with a 320 kb insert of genomic DNA which includes the HLA-A gene was used to screen a cDNA library of human duodenal mucosa. Seven cDNA clones were isolated which correspond to seven new non-HLA class I structural genes. These new genes are located within a region that may well contain the gene responsible for hemochromatosis and have therefore been named HCG I-VII (Hemochromatosis Candidate Gene). HCG I, III, V and VI are probably single copy genes, situated at 180, 155, 140 and 230 kb centromeric to HLA-A, respectively. HCG II, IV and VII present several copies: one copy of HCG II, one of HCG IV and one of HCG VII are centromeric to HLA-A (at 30, 70 and 100 kb respectively). Another copy of HCG IV is 20 kb telomeric to HLA-A. Each of the genes localized on the YAC B30 is associated with an CpG/HTF island.
el Kahloun
A
A
Laboratoire de Biochimie Médicale B, Faculté de Médecine, Rennes, France.
Chauvel
B
B
Mauvieux
V
V
Dorval
I
I
Jouanolle
A M
AM
Gicquel
I
I
Le Gall
J Y
JY
David
V
V
D015139:Blotting, Southern; D002460:Cell Line; D015825:Chromosomes, Fungal; D004247:DNA / Q000235:genetics*; D004386:Duodenum; D015723:Gene Library; D005796:Genes; D005805:Genes, MHC Class I; D015234:HLA-A Antigens / Q000235:genetics*; D006432:Hemochromatosis / Q000235:genetics; D006801:Humans; D007413:Intestinal Mucosa / Q000276:immunology; D009928:Organ Specificity; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics
D015234:HLA-A Antigens; D004247:DNA