A rapid fluorometric method for the determination of carnitine palmitoyltransferase.
A rapid and sensitive fluorometric assay for the measurement of carnitine palmitoyltransferase (CPT) activity is described. In this assay the coenzyme A (CoA) liberated from palmitoyl-CoA by CPT reacts with N-(9-acridinyl)-maleimide to form a fluorescent product. By comparing the fluorescence intensities of external CoA standards, CPT activity can be determined. The coefficients of variation within-run and between-run of the method were 6.9 and 10%, respectively. The CPT activity of human muscle samples measured with this assay is comparable to those obtained with a commonly used radiochemical assay and CPT deficiency in skeletal muscle from a patient was detected by this new fluorometric assay.
Schäfer
J
J
Department of Neurology, University of Würzburg, Germany.
Turnbull
D M
DM
Reichmann
H
H
D000818:Animals; D002331:Carnitine / Q000378:metabolism; D002334:Carnitine O-Palmitoyltransferase / Q000378:metabolism*; D002417:Cattle; D003065:Coenzyme A / Q000378:metabolism; D005470:Fluorometry / Q000379:methods; D006801:Humans; D008099:Liver / Q000201:enzymology; D008301:Maleimides / Q000378:metabolism; D009132:Muscles / Q000201:enzymology; D010171:Palmitoyl Coenzyme A / Q000378:metabolism; D051381:Rats; D015203:Reproducibility of Results
D008301:Maleimides; D010171:Palmitoyl Coenzyme A; C080490:N-(9-acridinyl)maleimide; D002334:Carnitine O-Palmitoyltransferase; D002331:Carnitine; D003065:Coenzyme A