Detailed characterization of a human 8q24.1 microdissection library and generation of "sequence-tagged sites".
A total of 533 clones from a human 8q24.1 microdissection library was analyzed by automatic DNA sequencing. Three hundred and thirty-seven different insert sequences were found. The insert size ranged from 45 to 376 bp (mean, 170 bp). Eighty-six percent (291/337) of these sequences were free of repetitive DNA. Each of 19 clones tested was successfully translated into a sequence-tagged site. We conclude that the microdissection library is a rich source of DNA markers for 8q24.1.
La Pillo
B
B
Institut für Humangenetik, Universitätsklinikum Essen, FRG.
Karpinski
S
S
Lüdecke
H J
HJ
Horsthemke
B
B
D001483:Base Sequence; D002874:Chromosome Mapping; D002898:Chromosomes, Human, Pair 8 / Q000648:ultrastructure*; D005819:Genetic Markers; D015698:Genomic Library; D006801:Humans; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D016324:Sequence Tagged Sites
D005819:Genetic Markers; D009838:Oligodeoxyribonucleotides