Analyses of volatile C2 haloethanes and haloethenes in tissues: sample preparation and extraction.
A tissue extraction procedure was developed which minimized loss of readily volatilizable compounds for subsequent quantification by headspace gas chromatography, and evaluated for perchloroethylene (PER), 1,1,1-trichloroethane, 1,1,2,2-tetrachloroethane, and 1,1,2-trichloroethylene. Of the procedures evaluated, joint isooctane and saline tissue homogenization had the most efficient recovery, ranging from 73 to 104% for the four halocarbons from seven different rat tissues. PER concentrations were also determined in tissues of rats following in vivo halocarbon administration. Recovery did not appear to be tissue-dependent, but did vary somewhat with test chemical, with the least volatile, most lipophilic compounds exhibiting the highest recovery.
Chen
X M
XM
Department of Pharmacology and Toxicology, College of Pharmacy, University of Georgia, Athens 30602-2356.
Dallas
C E
CE
Muralidhara
S
S
Srivatsan
V
V
Bruckner
J V
JV
D000818:Animals; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002849:Chromatography, Gas; D004980:Ethane / Q000031:analogs & derivatives / Q000032:analysis; D008401:Gas Chromatography-Mass Spectrometry; D006843:Hydrocarbons, Chlorinated / Q000032:analysis; D006846:Hydrocarbons, Halogenated / Q000032:analysis*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013750:Tetrachloroethylene / Q000032:analysis; D014018:Tissue Distribution; D014240:Trichloroethanes / Q000032:analysis; D014241:Trichloroethylene / Q000032:analysis
D006843:Hydrocarbons, Chlorinated; D006846:Hydrocarbons, Halogenated; D014240:Trichloroethanes; C024566:1,1,1-trichloroethane; C015530:1,1,2,2-tetrachloroethane; D014241:Trichloroethylene; D004980:Ethane; D013750:Tetrachloroethylene