Effect of streptozotocin-diabetes on the level of VIP mRNA in myenteric neurones.
The effect of streptozotocin-induced diabetes on the production of vasoactive intestinal polypeptide messenger RNA in the myenteric neurones of 8 weeks diabetic rat intestine was examined using in situ hybridization. Total ganglion cell number per length (mm) of tissue section was measured using NADH diaphorase histochemistry. Although ganglion cells were more numerous in the control preparations compared with diabetic samples, a significantly greater number of vasoactive intestinal polypeptide messenger RNA-containing cells was detected in the diabetic tissues. These observations suggest that there is either a decrease in the breakdown of vasoactive intestinal polypeptide messenger RNA or an increase in its synthesis in myenteric neurones of diabetic rat intestine.
Belai
A
A
Department of Anatomy and Developmental Biology and Centre for Neuroscience, University College London.
Facer
P
P
Bishop
A
A
Polak
J M
JM
Burnstock
G
G
D000818:Animals; D001835:Body Weight / Q000502:physiology; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D005724:Ganglia / Q000201:enzymology; D006651:Histocytochemistry; D017403:In Situ Hybridization; D008297:Male; D009197:Myenteric Plexus / Q000166:cytology / Q000378:metabolism*; D009252:NADPH Dehydrogenase / Q000032:analysis; D009474:Neurons / Q000378:metabolism*; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D017208:Rats, Wistar; D014660:Vasoactive Intestinal Peptide / Q000096:biosynthesis*
D012333:RNA, Messenger; D014660:Vasoactive Intestinal Peptide; D009252:NADPH Dehydrogenase