Expression of the gene encoding secreted placental alkaline phosphatase (SEAP) by a nondefective adenovirus vector.
A nondefective recombinant human adenovirus 5 (Ad5) carrying the SEAP gene, encoding human secreted placental alkaline phosphatase, in the E3 region of the Ad5 genome was obtained. The expression of SEAP at the early and late stages of Ad5 infection was demonstrated in permissive and semi-permissive cell cultures. The amount of SEAP in the culture medium of the 293 cells was 13.6% of the total protein.
Doronin
K K
KK
Institute of Agricultural Biotechnology, Moscow, Russian Federation.
Zakharchuk
A N
AN
Grinenko
N F
NF
Yurov
G K
GK
Krougliak
V A
VA
Naroditsky
B S
BS
D000260:Adenoviruses, Human / Q000201:enzymology / Q000235:genetics*; D000469:Alkaline Phosphatase / Q000235:genetics* / Q000378:metabolism; D002478:Cells, Cultured; D003001:Cloning, Molecular; D005822:Genetic Vectors; D006801:Humans; D007700:Kinetics; D010920:Placenta / Q000378:metabolism*; D010957:Plasmids
D000469:Alkaline Phosphatase