title: Confirmation of the airborne occurrence of micron-size airborne pollen antigen carrying particles by immunoblotting.
abstract: We confirmed the existence of micron-size airborne particles carrying major pollen antigen of Cryptomeria japonica (Cry j l) and Lolium perenne (Lol p l) by means of an immunoblotting technique and examination by light microscopy. The size of the spots on a nitrocellulose membrane, marking the presence of antigen on the sampler's tape, varied considerably under light microscopic examination. Spots smaller than pollen grains and micron-size spots were observed together with large spots which seem to have been derived from intact pollen grains. The number of micron-size spots counted under a light microscope did not correspond with the number of spots from pollen grain observable with the naked eyes. This suggests other sources of airborne Cry j l particles than intact pollen grains alone.
authors: Yamagata Prefectural Institute of Public Health, Japan.
authors: Y
authors: Y
authors: Takahashi
authors: M
authors: M
authors: Sakaguchi
authors: S
authors: S
authors: Inouye
authors: T
authors: T
authors: Nagoya
authors: M
authors: M
authors: Watanabe
authors: H
authors: H
authors: Yasueda
mesh_terms: D000388:Air; D000941:Antigens / Q000302:isolation & purification*; D015151:Immunoblotting; D008129:Lolium; D010316:Particle Size; D011058:Pollen
chemical_list: D000941:Antigens