Correlative light and electron microscopy of the same sections embedded in HPMA, Quetol 523 and MMA.
Semithin sections, cut from tissues stained with acid and basic dyes after embedding in 2-hydroxypropyl methacrylate, Quetol 523 and methyl methacrylate, showed cytoplasmic components at a high resolution by light microscopy. These same sections could then be viewed, after osmium tetroxide, uranyl and lead staining, by the electron microscope. These sections had a number of inherent advantages: they could be observed with a light microscope; they facilitated analysis of cellular structures in the identical sites, and they were frequently the optimum thickness to provide three-dimensional information. We clearly established the structural detail of this same-section correlative light-electron microscopy approach by showing that the coloured materials observed in such sections of cells followed the distribution of fine structures within the same sections as determined by electron microscopy. In some instances the fidelity of the correlation between the distribution of the coloured area and cytoplasmic components in identical cells of the same section revealed significant details which could not visualized in thin sections. This technique, therefore, provided a simple and useful solution to many problems that require the localization of cellular components in identical cells selected previously by light microscopy.
Kushida
T
T
Department of Anatomy, Tokyo Women's Medical College.
Nagato
Y
Y
Iijima
H
H
Kushida
H
H
D000818:Animals; D006652:Histological Techniques; D008689:Methacrylates; D051379:Mice; D008853:Microscopy; D008854:Microscopy, Electron; D016610:Tissue Embedding / Q000379:methods*
D008689:Methacrylates