Negative selection of thymus-dependent CD4+8+ intestinal intraepithelial lymphocytes by internal superantigens.
CD4 and CD8 double-positive (DP) T cells were transiently found in the Peyer's patch of mice 2 to 3 weeks after birth and preceded the appearance of DP intestinal intraepithelial lymphocytes (iIEL). DP iIEL were detected in euthymic-specific pathogen-free mice, but not in athymic and germ-free mice. The pattern of V beta genes expressed on these DP iIEL was similar to that of CD4 single-positive peripheral T cells, which reflected the negative selection by internal superantigens, M1s, and I-E. CD8 SP T cells did not receive such a strict negative selection with these antigens. DP iIEL expressed a high density of T cell receptor alpha beta and CD3 and responded to immobilized anti-CD3 to undergo blastogenesis and de novo IL2 receptor expression. These results indicate that DP iIEL are mature thymus-dependent T cells immigrated to intraepithelial space via the Peyer's patch in response to intestinal antigenic stimulation.
Nagasawa
T
T
Department of Periodontology, School of Dentistry, Tokyo Medical and Dental University, Japan.
Sano
K
K
Ike
F
F
Kishimoto
H
H
Nakayama
T
T
Asano
Y
Y
Ishikawa
I
I
Tada
T
T
D000818:Animals; D015704:CD4 Antigens / Q000032:analysis / Q000276:immunology*; D016827:CD8 Antigens / Q000032:analysis / Q000276:immunology*; D004848:Epithelium / Q000276:immunology; D005260:Female; D005856:Germ-Free Life; D007150:Immunohistochemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D010581:Peyer's Patches / Q000254:growth & development / Q000276:immunology*; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000032:analysis / Q000276:immunology*; D013047:Specific Pathogen-Free Organisms; D013601:T-Lymphocytes / Q000276:immunology*
D015704:CD4 Antigens; D016827:CD8 Antigens; D016693:Receptors, Antigen, T-Cell, alpha-beta