title: Nonradioactive assay for adenosine 5'-phosphosulfate sulfotransferase using reversed-phase ion-pair high-performance liquid chromatography.
abstract: A nonradioactive and simple method for the assay of adenosine 5'-phosphosulfate sulfotransferase (APSSTase) is described. The method is based upon the quantitative measurement of the substrate adenosine 5'-phosphosulfate and/or product AMP by ion-pair reversed-phase high-performance liquid chromatography on a C18 column. Isocratic elution renders the method simple and fast. This method is standardized for partially purified APSSTase from the marine red macroalga Porphyra yezoensis.
authors: School of Fisheries Sciences, Kitasato University, Iwate, Japan.
authors: N
authors: N
authors: Kanno
authors: E
authors: E
authors: Nagahisa
authors: M
authors: M
authors: Sato
authors: Y
authors: Y
authors: Sato
mesh_terms: D002851:Chromatography, High Pressure Liquid; D000461:Rhodophyta / Q000201:enzymology*; D015238:Sulfotransferases / Q000032:analysis*
chemical_list: D015238:Sulfotransferases; C081239:adenosine 5'-phosphosulfate sulfotransferase