A cloned replicon of Saccharopolyspora phages JHJ-1 and JHJ-3 is stably maintained as a plasmid in various actinomycetes.
A replicon of phage JHJ-1 (and JHJ-3) was cloned. The autonomously replicating phage element was maintained as a medium-copy-number shuttle plasmid in many actinomycetes, and was efficiently transmitted to spores without antibiotic selection. One gene was shown to be expressed in a vector containing the JHJ-3 replicon.
Gaudreau
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LR
Département de Biologie, Université de Sherbrooke, Québec, Canada.
Déry
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CV
D000192:Actinomycetales / Q000235:genetics; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001435:Bacteriophages / Q000235:genetics*; D003001:Cloning, Molecular; D005798:Genes, Bacterial; D005822:Genetic Vectors; D006026:Glycoside Hydrolases / Q000235:genetics; D010957:Plasmids; D012093:Replicon; D015183:Restriction Mapping; D016930:Saccharopolyspora / Q000235:genetics*; D013171:Spores, Bacterial
D000900:Anti-Bacterial Agents; D006026:Glycoside Hydrolases; C019868:chitosanase