Rapid fluorometric measurement of the intra-cellular concentration of ciprofloxacin in mouse peritoneal macrophages.
A simple fluorometric assay requiring only a single sample of cells to determine the number of cells, from the DNA linked to the fluorochrome 4',6-diamidino-2-phenylindol (DAPI), and the uptake of ciprofloxacin, a natural fluorescent quinolone is described. Mouse peritoneal macrophages were found to concentrate ciprofloxacin up to 12.7 (+/- 1.5)-fold. Combined fluorometry provides a precise, sensitive method for determining the intracellular concentration of fluoroquinolones, as well as that of naturally fluorescent or fluorochrome-linked drugs.
Château
M T
MT
INSERM U65, Université Montpellier II, France.
Caravano
R
R
D000818:Animals; D002939:Ciprofloxacin / Q000032:analysis* / Q000493:pharmacokinetics; D004247:DNA / Q000032:analysis / Q000302:isolation & purification; D066298:In Vitro Techniques; D008264:Macrophages / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure; D051379:Mice; D010529:Peritoneal Cavity / Q000166:cytology; D010587:Phagocytosis / Q000187:drug effects; D013050:Spectrometry, Fluorescence
D002939:Ciprofloxacin; D004247:DNA