High resolution polyacrylamide gel electrophoresis of Physarum polycephalum histones: improved resolution of variants and modified forms.
An electrophoresis system providing a good resolution of all basic proteins and their variants or modified forms extracted from Physarum polycephalum nuclei was developed. This two-dimensional polyacrylamide gel electrophoresis system combines the use of an AUT gel containing a preformed gradient of urea in the first dimension and a SDS polyacrylamide gel in the second one.
Albert
P
P
Laboratoire de Biologie Cellulaire, Université de Reims Champagne-Ardenne, France.
Lacorre-Arescaldino
I
I
Toublan
B
B
D000818:Animals; D002499:Centrifugation, Density Gradient; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D005656:Fungal Proteins / Q000032:analysis* / Q000302:isolation & purification; D006657:Histones / Q000032:analysis* / Q000302:isolation & purification; D007536:Isomerism; D016839:Physarum polycephalum / Q000737:chemistry*; D015800:Protozoan Proteins / Q000032:analysis* / Q000302:isolation & purification; D012967:Sodium Dodecyl Sulfate; D014508:Urea
D005656:Fungal Proteins; D006657:Histones; D015800:Protozoan Proteins; D012967:Sodium Dodecyl Sulfate; D014508:Urea