A proposed nonpathogenic biological indicator for thermal inactivation of Listeria monocytogenes.
Listeria innocua M1 was developed as a thermal processing indicator organism for L. monocytogenes by selection of a rifampin- and streptomycin-resistant mutant. zetaD values were 5.6 and 5.8 degrees C, and D (68 degrees C) values were 3.8 and 4.9 s for L. monocytogenes and L. innocua, respectively, in skim milk. The advantages of easy selection, similar heat resistance, and nonpathogenicity make L. innocua M1 appropriate for challenge studies designed to evaluate process lethality with respect to L. monocytogenes.
Fairchild
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Department of Food Science, North Carolina State University, Box 7624, Raleigh 27695-7624.
Foegeding
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D000818:Animals; D003470:Culture Media; D004352:Drug Resistance, Microbial / Q000235:genetics; D005260:Female; D005516:Food Microbiology; D005519:Food Preservation; D006358:Hot Temperature; D008087:Listeria / Q000235:genetics / Q000472:pathogenicity / Q000502:physiology*; D008089:Listeria monocytogenes / Q000472:pathogenicity / Q000502:physiology*; D051379:Mice; D008892:Milk / Q000382:microbiology; D009154:Mutation; D010957:Plasmids
D003470:Culture Media