Anti-inflammatory actions of an N-terminal peptide from human lipocortin 1.
An acetylated polypeptide corresponding to residues 2-26 of human lipocortin 1 was synthesized and the anti-inflammatory activity assessed in three models of acute inflammation in rat and mouse. In the carrageenin rat paw oedema test, the peptide produced a maximal inhibition of approximately 41% at the 3 h time point with a 10 micrograms dose. When rat paw oedema was induced by the injection of venom phospholipase A2, the peptide produced a significant inhibition (31%) at the top dose of 20 micrograms per paw. In the mouse air-pouch model, systemic treatment with the peptide produced a dramatic reduction in cytokine-induced leukocyte migration with an ID50 of approximately 40 micrograms per mouse. The N-terminal peptide 2-26 shares the actions of lipocortin 1 in these acute models of inflammation.
Cirino
G
G
Department of Experimental Pharmacology, University of Naples-Federico II, Italy.
Cicala
C
C
Sorrentino
L
L
Ciliberto
G
G
Arpaia
G
G
Perretti
M
M
Flower
R J
RJ
D000595:Amino Acid Sequence; D000818:Animals; D017305:Annexin A1 / Q000494:pharmacology*; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D002351:Carrageenan; D004487:Edema / Q000139:chemically induced / Q000517:prevention & control; D007375:Interleukin-1 / Q000494:pharmacology; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D009504:Neutrophils / Q000187:drug effects; D010455:Peptides / Q000494:pharmacology*; D010740:Phospholipases / Q000037:antagonists & inhibitors*; D010741:Phospholipases A; D054467:Phospholipases A2; D051381:Rats; D017208:Rats, Wistar
D017305:Annexin A1; D000894:Anti-Inflammatory Agents, Non-Steroidal; D007375:Interleukin-1; D010455:Peptides; D002351:Carrageenan; D010740:Phospholipases; D010741:Phospholipases A; D054467:Phospholipases A2