Enzymatic modification of plasma low density lipoproteins in rabbits: a potential treatment for hypercholesterolemia.
Phospholipase A2 (EC 3.1.1.4) hydrolyzes certain phospholipids of low density lipoprotein (LDL). Plasma clearance of phospholipase A2-modified human LDL is up to 17 times faster than that of native human LDL in hypercholesterolemic rabbits. Modification of blood lipoproteins of hypercholesterolemic rabbits was performed by using an extracorporeal circuit containing immobilized phospholipase A2. After 90-min treatments, nearly 30% decreases in plasma cholesterol concentrations were observed. Erythrocyte, leukocyte, and platelet counts showed no net change after treatment. This technique does not require any fluid replacement or sorbent regeneration and offers a potential approach for lowering serum cholesterol and LDL levels.
Labeque
R
R
Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge 02139.
Mullon
C J
CJ
Ferreira
J P
JP
Lees
R S
RS
Langer
R
R
D000818:Animals; D001011:Aorta / Q000473:pathology; D001161:Arteriosclerosis / Q000473:pathology / Q000503:physiopathology; D001646:Bile / Q000378:metabolism; D005112:Extracorporeal Circulation; D006801:Humans; D006937:Hypercholesterolemia / Q000097:blood / Q000378:metabolism / Q000628:therapy*; D008077:Lipoproteins, LDL / Q000097:blood / Q000493:pharmacokinetics*; D008657:Metabolic Clearance Rate; D010741:Phospholipases A / Q000378:metabolism* / Q000627:therapeutic use*; D054467:Phospholipases A2; D010743:Phospholipids / Q000032:analysis / Q000378:metabolism; D011817:Rabbits; D014018:Tissue Distribution
D008077:Lipoproteins, LDL; D010743:Phospholipids; D010741:Phospholipases A; D054467:Phospholipases A2