title: Studies of the relationship between cell proliferation and cell death. III. AP-1 DNA-binding activity during concanavalin A-induced proliferation or dexamethasone-induced apoptosis of rat thymocytes.
abstract: In previous papers we started to investigate the possible relationship existing between cell proliferation and cell death. We reported that in rat thymocytes, undergoing cell proliferation upon stimulation with Concanavalin A or cell death following dexamethasone treatment, an induction of c-fos and c-jun occurs with different but partially overlapping kinetics (Grassilli et al., BBRC, 1992, 188, 1261-1266). To test the hypothesis that, in both processes, the early induction of these nuclear oncogenes is necessary to allow the induction of later-expressed genes regulated at the transcriptional level by AP-1 complex, AP-1 DNA-binding activity was investigated in the aforementioned model. Indeed, we found that AP-1 is activated not only in proliferating but also in apoptotic cells, with a kinetics in accord with our data about c-fos and c-jun mRNA accumulation in the same experimental model. Moreover, our findings suggest that an interaction between AP-1 and glucocorticoid receptor also occurs in our model of dexamethasone-mediated apoptosis.
authors: Istituto di Patologia Generale, Università di Modena, Italy.
authors: E
authors: E
authors: Sikora
authors: E
authors: E
authors: Grassilli
authors: E
authors: E
authors: Bellesia
authors: L
authors: L
authors: Troiano
authors: C
authors: C
authors: Franceschi
mesh_terms: D000818:Animals; D016923:Cell Death / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D003208:Concanavalin A / Q000494:pharmacology*; D004247:DNA / Q000378:metabolism*; D003907:Dexamethasone / Q000494:pharmacology*; D016755:Proto-Oncogene Proteins c-jun / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction; D013950:Thymus Gland / Q000166:cytology / Q000187:drug effects*
chemical_list: D016755:Proto-Oncogene Proteins c-jun; D003208:Concanavalin A; D003907:Dexamethasone; D004247:DNA