Intracellular changes of HeLa cells after single or repeated treatment with cytostatics.
HeLa cells were treated once or repeatedly using the cytostatics doxorubicin (adriamycin, Ad, CAS 23214-92-8), cisplatin (Pt, CAS 15663-27-1) and fluorouracil (FU, CAS 51-21-8). Intracellular GSH (reduced glutathione) contents, activities of protein kinase C, cytotoxicity and membrane fluidity were investigated. During single treatment protein kinase C activities as well as membrane fluidity increased, whereas intracellular GSH decreased. With repeated treatments protein kinase C activities increased further. Membrane fluidity as well as intracellular GSH contents increased. The investigated parameters may be correlated with sensitivity of cells against cytostatics.
Busse
E
E
Abteilung für Hämatologie und Onkologie, Universität Frankfurt/Main, Fed. Rep. of Germany.
Zimmer
G
G
Kornhuber
B
B
D000970:Antineoplastic Agents / Q000494:pharmacology*; D001973:Bromodeoxyuridine / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D002462:Cell Membrane / Q000187:drug effects; D002945:Cisplatin / Q000494:pharmacology; D003545:Cysteine / Q000378:metabolism; D004317:Doxorubicin / Q000494:pharmacology; D005472:Fluorouracil / Q000494:pharmacology; D005978:Glutathione / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D008560:Membrane Fluidity / Q000187:drug effects*; D008566:Membranes / Q000187:drug effects; D011493:Protein Kinase C / Q000378:metabolism*
D000970:Antineoplastic Agents; D004317:Doxorubicin; D011493:Protein Kinase C; D001973:Bromodeoxyuridine; D005978:Glutathione; D003545:Cysteine; D002945:Cisplatin; D005472:Fluorouracil