Isolation and mapping of human chromosome 21 cosmids using a probe for RTVL-H retrovirus-like elements.
We have explored the usefulness of a cloned low-repetitive DNA element, termed RTVL-H, for the region-specific isolation of DNA cosmids from chromosome 21. Hybridization of the RTVL-H element as a probe to genomic Southern blots of DNAs from a chromosome 21-specific somatic cell hybrid panel demonstrated the presence of at least seven RTVL-H-related sequences in defined regions dispersed over the chromosome. Some of the RTVL-H sequences detected by using the chromosome 21-specific somatic cell hybrid panel were subsequently isolated from a chromosome 21-specific cosmid library and mapped using somatic cell hybrids. In general, close correspondence was found between mapping results obtained using the consensus RTVL-H probe on the chromosome 21-specific somatic cell hybrid panel and the more precise mapping obtained using cosmids. The results demonstrate that sequences such as the RTVL-H element can be used for isolating region-specific sequences.
Meulenbelt
I
I
INGENY B.V., Leiden, The Netherlands.
Wapenaar
M C
MC
Patterson
D
D
Vijg
J
J
Uitterlinden
A G
AG
D000818:Animals; D002874:Chromosome Mapping / Q000379:methods*; D002891:Chromosomes, Human, Pair 21; D003360:Cosmids; D006224:Cricetinae; D015342:DNA Probes; D006801:Humans; D006822:Hybrid Cells; D009693:Nucleic Acid Hybridization; D012091:Repetitive Sequences, Nucleic Acid; D012190:Retroviridae / Q000235:genetics*
D015342:DNA Probes