Production of stable human-mouse hybridomas secreting monoclonal antibodies against Rh D and c antigens.
Peripheral blood lymphocytes from donors immunized against Rh antigens were fused with mouse myelomas and heteromyelomas in order to obtain human-mouse hybridomas secreting antibodies specific for these antigens. Three cell lines secreting anti-D IgG and two secreting anti-c IgM were stabilized and produced immunoglobulins for several months. These human monoclonal antibodies were evaluated as reagents for Rh phenotyping. Their complementary activity towards weak D and partial D antigens is examined.
Rapaille
A
A
Centre de Transfusion Sanguine, Croix Rouge de Belgique.
François-Gérard
C
C
Donnay
D
D
Sondag-Thull
D
D
D000818:Animals; D000911:Antibodies, Monoclonal; D000941:Antigens / Q000097:blood*; D001402:B-Lymphocytes / Q000276:immunology; D002459:Cell Fusion; D006801:Humans; D006825:Hybridomas / Q000276:immunology*; D007074:Immunoglobulin G / Q000378:metabolism; D007075:Immunoglobulin M / Q000378:metabolism; D007518:Isoantibodies / Q000097:blood*; D051379:Mice; D009101:Multiple Myeloma / Q000276:immunology; D012204:Rh-Hr Blood-Group System / Q000276:immunology*; D012698:Serologic Tests
D000911:Antibodies, Monoclonal; D000941:Antigens; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007518:Isoantibodies; C033242:RH-antibodies; D012204:Rh-Hr Blood-Group System