Acetaldehyde-collagen adducts in N-nitrosodimethylamine-induced liver cirrhosis in rats.
Increased acetaldehyde levels have been found in non-alcoholic liver diseases and an acetaldehyde-collagen adduct has been reported in rats with CCl4-induced cirrhosis. In cytosol and microsomes of rats with cirrhosis produced by N-nitrosodimethylamine, a similar acetaldehyde-protein adduct of approximately 200 kD was recognized by rabbit IgG raised against either an in vitro produced hemocyanin-acetaldehyde adduct or an in vivo occurring P4502E1-acetaldehyde adduct isolated from alcohol-fed rats, as well as by anti-rat collagen (I) IgG. Its immune complexes contained 3 proteins that reacted with the anti-collagen IgG and were digested by collagenase: 2 proteins with molecular weights similar to procollagens alpha 1 and alpha 2, and a beta 1,2(I)-like protein which was readily produced by in vitro modification of cytosol with acetaldehyde.
Baraona
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Alcohol Research and Treatment Center, Veterans Affairs Medical Center, Bronx, New York.
Liu
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W
Ma
X L
XL
Svegliati-Baroni
G
G
Lieber
C S
CS
D000079:Acetaldehyde / Q000378:metabolism*; D000818:Animals; D003094:Collagen / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism; D004128:Dimethylnitrosamine / Q000008:administration & dosage / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D008099:Liver / Q000187:drug effects*; D008106:Liver Cirrhosis, Experimental / Q000139:chemically induced* / Q000378:metabolism*; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
D003094:Collagen; D000079:Acetaldehyde; D004128:Dimethylnitrosamine