Differential growth control of normal, preneoplastic and neoplastic Syrian hamster embryo cells in serum-free media.
The objective of this work was to develop a serum-free medium that supported the growth of secondary Syrian hamster embryo (SHE) cells suitable for use in the morphological transformation assay (MTA). The current assay employs high levels of fetal bovine serum, each lot of which must be selected for its efficacy in the assay. To circumvent the problems associated with the use of serum, two serum-free media (CGM-4 and CGM-9) were developed. The approach used was to replace serum with more defined hormones and growth factors. DME/F12 with low NaHCO3 (pH 6.7) was chosen as a basal nutrient medium since this pH has been reported to give enhanced transformation. Both serum-free media supported the clonal high density growth of normal secondary SHE cells. These media were also found to support the growth of several established SHE cell lines exhibiting successive stages of neoplastic progression. However, significant differential responses of the individual cell lines to CGM-4 and CGM-9 were observed, whereas, in serum-supplemented medium all three lines responded similarly. These results raise the hope that CGM-4 and CGM-9 will be useful for studies of altered autocrine function during transformation and progression as well as for use in the transformation assay itself.
Kaighn
M E
ME
Biological Research Faculty and Facility, Inc., Ijamsville, MD 21754.
Kumari
H L
HL
Spalding
J W
JW
Barrett
J C
JC
Iype
P T
PT
D000818:Animals; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D006224:Cricetinae; D016895:Culture Media, Serum-Free; D006133:Growth Substances / Q000494:pharmacology*; D008647:Mesocricetus / Q000196:embryology*; D011230:Precancerous Conditions / Q000473:pathology; D014407:Tumor Cells, Cultured
D016895:Culture Media, Serum-Free; D006133:Growth Substances