Delineation of translocation t(15; 17) in acute promyelocytic leukemia by chromosomal in situ suppression hybridization.
In this study we demonstrate the feasibility of chromosomal in situ suppression (CISS) hybridization to detect the translocation t(15; 17) in metaphase spreads of patients with acute promyelocytic leukemia. Using DNA libraries from sorted human chromosomes 15 and 17 the translocation t(15; 17) can be unequivocally identified even if the spread and the morphology of the chromosomes are poor. The sensitivity of CISS hybridization is compared with the sensitivity of conventional G-banded karyotypes.
Speicher
M R
MR
University of Heidelberg, Institute for Human Genetics and Anthropology, Germany.
Jauch
A
A
Parr
A
A
Becher
R
R
D000328:Adult; D002871:Chromosome Banding; D002884:Chromosomes, Human, Pair 15 / Q000502:physiology*; D002886:Chromosomes, Human, Pair 17 / Q000502:physiology*; D004273:DNA, Neoplasm / Q000235:genetics; D005260:Female; D015723:Gene Library; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods*; D007621:Karyotyping; D015473:Leukemia, Promyelocytic, Acute / Q000235:genetics*; D008297:Male; D008677:Metaphase / Q000502:physiology; D008875:Middle Aged; D012680:Sensitivity and Specificity; D014178:Translocation, Genetic
D004273:DNA, Neoplasm