Constitutive expression and production of tumor necrosis factor-beta in T-cell lines infected with HTLV-I and HTLV-II.
A highly sensitive enzyme-linked immunoassay was developed to detect soluble levels of TNF-beta. Analysis of TNF-beta in culture supernatants from long-term T-cell lines infected with human T-cell lymphotropic virus (HTLV) types I and II demonstrated elevated levels of TNF-beta when compared with uninfected T-cell lines. Presence of interleukin-2 in the culture medium showed a synergistic effect upon TNF-beta production. Further, constitutive expression of TNF-beta mRNA was observed in most cell lines infected with HTLV-I or HTLV-II. These results demonstrate that infections with HTLV-I/II can alter production of TNF-beta, presumably via the transactivation of TNF-beta promoter by the tax protein of HTLV-I and HTLV-II.
Nakajima
T
T
University of Montreal, Research Center Notre-Dame Hospital, Quebec, Canada.
Kitamura
K
K
Yamashita
N
N
Sakane
T
T
Mizushima
Y
Y
Delespesse
G
G
Lal
R B
RB
D002460:Cell Line; D003429:Cross Reactions; D016356:Gene Products, tax / Q000235:genetics; D015368:Human T-lymphotropic virus 1 / Q000502:physiology*; D015367:Human T-lymphotropic virus 2 / Q000502:physiology*; D006801:Humans; D009500:Neutralization Tests; D011401:Promoter Regions, Genetic; D013601:T-Lymphocytes / Q000378:metabolism / Q000382:microbiology*; D014158:Transcription, Genetic; D015533:Transcriptional Activation; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis* / Q000235:genetics
D016356:Gene Products, tax; D014409:Tumor Necrosis Factor-alpha