[Differential expression of recombinant hybrid proteins containing amino- and carboxy-terminal regions of the exterior large glycoprotein (gp46) of the human T-cell leukemia virus (HTLV-I) in Escherichia coli cells].
Plasmid clones capable of expressing a recombinant fusion proteins containing the anthranilate synthase of E. coli (TrpE) and different regions of gp46 HTLV-I were constructed on the basis of pATH-vectors. A high extent of TrpE-gp46 proteolytic degradation took place independently of the bacterial La-protease. Fusion proteins containing an N-terminal part of gp46 were more stable and could be purified in preparative quantities but were less antigenic. On the contrary, a TrpE-gp46 protein encoded by the TaqI-TaqI DNA fragment and containing only 35 C-terminal amino acids was still susceptible to degradation but possessed good serologic reactivity. Some of the recombinant proteins obtained can be useful for diagnostics and for preparing monoclonal or polyclonal antibodies.
Pavlish
O A
OA
Syrtsev
A V
AV
Shcherbak
L N
LN
Gurtsevich
V E
VE
D000878:Anthranilate Synthase / Q000737:chemistry; D004274:DNA, Recombinant; D004926:Escherichia coli; D015870:Gene Expression; D015686:Gene Products, env / Q000737:chemistry*; D015368:Human T-lymphotropic virus 1 / Q000737:chemistry*; D006868:Hydrolysis; D010957:Plasmids; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D015682:Retroviridae Proteins, Oncogenic / Q000737:chemistry*
D004274:DNA, Recombinant; D015686:Gene Products, env; D011993:Recombinant Fusion Proteins; D015682:Retroviridae Proteins, Oncogenic; C055266:gp46 protein, Human T-cell leukemia virus type I; D000878:Anthranilate Synthase
Differentsial'naia ékspressiia rekombinantnykh gibridnykh belkov, soderzhashchikh amino- i karboksiterminal'nuiu oblasti naruzhnogo bol'shogo glikoproteida (gp46) virusa T-kletochnogo leĭkoza cheloveka (HTLV-I) b kletkakh Escherichia coli.