[Three mutant forms of ribosomal protein L7/L12].
Three mutant forms of the ribosomal protein L7/L12 with Ser1, Met14, and Met26 replacements with Tyr were constructed for studying the N-terminal domain of the protein. Three point mutations in the gene L7/L12 on the phage M13mp18 were generated. Recombinant plasmids containing the mutant genes were constructed on the base of expression vector pKK223-3 and plasmid pUC19. The mutant proteins were expressed in Escherichia coli cells, and methods of their purification were developed. It was found that the mutant proteins L7/L12 do not differ from the wild-type protein L7/L12 and represent a suitable object for 1H-NMR study of the L7/L12 protein. The three mutant proteins bind with the E. coli ribosome.
Todorova
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D004926:Escherichia coli / Q000648:ultrastructure; D009682:Magnetic Resonance Spectroscopy; D016297:Mutagenesis, Site-Directed; D010957:Plasmids; D017354:Point Mutation; D012269:Ribosomal Proteins / Q000235:genetics* / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism
D012269:Ribosomal Proteins; C032493:ribosomal protein L17; C016700:ribosomal protein L7-L12
Tri mutantny formy ribosomnogo belka L7/L12.