title: Effect of lentinan on lymphocyte subsets of peripheral blood, lymph nodes, and tumor tissues in patients with gastric cancer.
abstract: The effect of lentinan on lymphocyte subsets of peripheral blood, lymph nodes, and tumor tissues in gastric cancer patients was investigated. A 2-mg dose of lentinan was administered to 12 patients intravenously twice, the first at 3-9 days before and the second the day before surgery. The results were then compared with a control group without lentinan administration comprising 12 patients. Regarding peripheral blood and lymph nodes without metastasis, lymphocyte subsets defined with anti-CD3, anti-CD4, anti-CD8, anti-Leu7, and anti-Leu11 were analyzed by flow-cytometry. As for tumor tissues, lymphocyte subsets defined with anti-CD3, anti-CD4, anti-CD8, anti-Leu11, and anti-M3 were analyzed after immunohistochemical staining. There were no significant changes in the lymphocyte subsets of peripheral blood between the two groups. In the lymph nodes, the CD4 cell ratios increased; otherwise in regard to tumor-infiltrating lymphocytes (TILs), the number of CD4, Leu11 and LeuM3 cells showed a prominent increase. Therefore, lentinan was observed to produce different effects in accordance with the subjective organs.
authors: First Department of Surgery, Tokyo Medical and Dental University, School of Medicine, Japan.
authors: K
authors: K
authors: Takeshita
authors: S
authors: S
authors: Watanuki
authors: M
authors: M
authors: Iida
authors: N
authors: N
authors: Saito
authors: M
authors: M
authors: Maruyama
authors: M
authors: M
authors: Sunagawa
authors: H
authors: H
authors: Habu
authors: M
authors: M
authors: Endo
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D007912:Lentinan / Q000494:pharmacology*; D008198:Lymph Nodes / Q000378:metabolism*; D016131:Lymphocyte Subsets / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D013274:Stomach Neoplasms / Q000097:blood / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology
chemical_list: D007912:Lentinan