title: Molecular cloning and expression of yellowfin porgy (Acanthopagrus latus houttuyn) growth hormone cDNA.
abstract: 1. The growth hormone cDNA of yellowfin porgy (ypGH cDNA) consisted of 915 base pairs. 2. The deduced amino acid (aa) sequence showed that the pre-GH comprised 204 residues, of which the first 17 residues formed a signal peptide. 3. Comparison of aa sequence of ypGH to seabream, tuna, rainbow trout and chum salmon showed that ypGH shared 95.1, 94.1, 65.3 and 62.4% homology with these species, respectively. 4. By expressing the ypGH cDNA in E. coli, a polypeptide around 23 kilodaltons (kDa) was found which was immunoreactive to GH antibody.
authors: Institute of Fisheries Science, National Taiwan University, Taipei, Republic of China.
authors: H J
authors: HJ
authors: Tsai
authors: K L
authors: KL
authors: Lin
authors: T T
authors: TT
authors: Chen
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D003062:Codon; D004247:DNA / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D005399:Fishes / Q000235:genetics*; D015870:Gene Expression; D013006:Growth Hormone / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D010957:Plasmids; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins; D012091:Repetitive Sequences, Nucleic Acid; D012474:Salmon; D017386:Sequence Homology, Amino Acid; D014169:Transformation, Bacterial; D014337:Trout; D014413:Tuna
chemical_list: D003062:Codon; D011994:Recombinant Proteins; D013006:Growth Hormone; D004247:DNA