title: Dietary lipid profile is a determinant of tissue phospholipid fatty acid composition and rate of weight gain in rats.
abstract: Modifications in membrane fatty acid composition and insulin action are possible through dietary intervention. We examined the metabolic fate of (n-3) fatty acids in male Wistar rats, using three isocaloric, high fat diets. The ET-L, OL-L and SAF-L diets contained edible tallow, olive oil and safflower oil, respectively, with identical amounts of (n-3) fatty acids as linseed oil. Despite isocaloric feeding, weight gain was lower (P < 0.001) in rats fed the more highly saturated ET-L diet (69 +/- 8 g) than in those fed either the high (n-9) fatty acid OL-L diet (93 +/- 2 g) or the high (n-6) fatty acid SAF-L diet (108 +/- 4 g). Analysis of red quadricep fatty acid composition revealed phospholipid (n-3) fatty acid levels in the ET-L-fed group (21.6 +/- 0.8 g/100 g fatty acids) to be significantly higher than in either the OL-L-fed (17.7 +/- 0.6 g/100 g fatty acids, P < 0.05) or SAF-L-fed (15.3 +/- 0.7 g/100 g fatty acids, P < 0.05) group. A similar pattern was observed in other muscles and white adipose tissue. A follow-up study using 14C-labeled (n-3) fatty acids in the diet showed greater (n-3) fatty acid incorporation in the ET-L-fed group relative to the other two groups and conversely lower 14CO2 production than in the SAF-L-fed group. These results demonstrate that metabolic fate of dietary fatty acids is strongly influenced by the overall fatty acid profile of the diet.(ABSTRACT TRUNCATED AT 250 WORDS)
authors: Garvan Institute of Medical Research, St Vincent's Hospital, Sydney, NSW, Australia.
authors: D A
authors: DA
authors: Pan
authors: L H
authors: LH
authors: Storlien
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism; D000818:Animals; D004041:Dietary Fats / Q000008:administration & dosage*; D002149:Energy Intake; D005223:Fats / Q000008:administration & dosage; D005227:Fatty Acids / Q000378:metabolism*; D015525:Fatty Acids, Omega-3 / Q000378:metabolism; D008043:Linseed Oil / Q000008:administration & dosage; D008297:Male; D009132:Muscles / Q000378:metabolism; D000069463:Olive Oil; D010084:Oxidation-Reduction; D010743:Phospholipids / Q000378:metabolism*; D010938:Plant Oils / Q000008:administration & dosage; D051381:Rats; D017208:Rats, Wistar; D012450:Safflower Oil / Q000008:administration & dosage; D014280:Triglycerides / Q000378:metabolism; D015430:Weight Gain
chemical_list: D004041:Dietary Fats; D005223:Fats; D005227:Fatty Acids; D015525:Fatty Acids, Omega-3; D000069463:Olive Oil; D010743:Phospholipids; D010938:Plant Oils; D014280:Triglycerides; D012450:Safflower Oil; D008043:Linseed Oil; C013698:tallow