Morphological changes induced by colchicine in the chick optic cup in early stages of development. A stereological study.
In this study chick embryo optic cups at HH stage 13 of development were analyzed under normal conditions and after inoculation with colchicine for 1, 2, 4, and 8 h. Several changes were seen after these periods of treatment: 1) modifications of the structure, with thicker regions in the cup and a general decrease in the total volume according to the duration of exposure to the drug (about 4 times less than normal, 5,035 x 10(3) microns 3 vs 1,334 x 10(3) microns 3 after 8 h of treatment); 2) enlargement of the ventricular cavity and its closure, due to failure of approximation of retinal and pigmentary layers; 3) failure of lens development, with delay and impairment of pit formation and deformation of all structures; lens volume was less than normal (about 4 times less, 2,148 x 10(3) microns 3 vs 658 x 10(3) microns 3 after 8 h of treatment); 4) a general segregation of the cells making up the structure, principally in the more active proliferating zones. The local alterations found are described.
Abadía-Molina
F
F
Department of Cell Biology, Faculty of Sciences, University of Granada, Spain.
Calvente
R
R
Carmona
R
R
Luján
R
R
Abadía-Fenoll
F
F
D000818:Animals; D002642:Chick Embryo; D003078:Colchicine / Q000494:pharmacology*; D005123:Eye / Q000033:anatomy & histology / Q000187:drug effects* / Q000196:embryology; D006651:Histocytochemistry; D007908:Lens, Crystalline / Q000033:anatomy & histology / Q000187:drug effects / Q000196:embryology; D012160:Retina / Q000033:anatomy & histology / Q000187:drug effects / Q000196:embryology; D013997:Time Factors
D003078:Colchicine