Co-expression of Fos immunoreactivity in protein kinase (PKC gamma)-positive neurones: quantitative analysis of a brain region involved in learning.
The expression of the gamma protein kinase C isoenzyme (PKC gamma) and of the c-fos immediate early gene protein product Fos in the intermediate and medial hyperstriatum ventrale (IMHV) of day-old chicks was determined immunocytochemically. Previous research has shown that (a) there is a learning-related increase in the expression of Fos in the IMHV of the chick after imprinting; (b) PKC gamma is expressed in neurones in most regions of the chick forebrain, including the IMHV. In the present study it was found that in imprinted chicks, 96.5% of neurones in the IMHV that expressed Fos also stained positively for PKC gamma. These results raise the possibility of a functional connection between PKC gamma activation and c-fos expression in neurones in general, and in particular in neurones in the IMHV that are involved in learning.
Ambalavanar
R
R
University of Cambridge, Department of Zoology, UK.
Van der Zee
E A
EA
Bolhuis
J J
JJ
McCabe
B J
BJ
Horn
G
G
D000818:Animals; D000911:Antibodies, Monoclonal; D002467:Cell Nucleus / Q000378:metabolism; D002645:Chickens; D007527:Isoenzymes / Q000378:metabolism*; D007858:Learning / Q000502:physiology*; D009474:Neurons / Q000378:metabolism*; D011493:Protein Kinase C / Q000378:metabolism*; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D007527:Isoenzymes; D016760:Proto-Oncogene Proteins c-fos; D011493:Protein Kinase C