Refrigerated storage of lyophilized and rehydrated, lyophilized human red cells.
Human red cells (RBCs) were collected in CPDA-1 and then freeze-dried in lyoprotective solution. The lyophilized RBCs were then stored at -20 degrees C for 7 days. At the end of the storage period, the lyophilized RBCs were rehydrated and washed in dextrose saline. The washed, reconstituted, lyophilized RBCs were resuspended in final wash solutions of ADSOL, CPDA-1, or a special additive solution containing glucose, citrate, phosphate, adenine, and mannitol, and then they were stored at 4 degrees C for an additional 7 days. The main purpose of this study was to determine whether human RBCs can be lyophilized in such a manner that normal metabolic, rheologic, and cellular properties are maintained during rehydration and subsequent storage in standard blood bank preservative solutions. Our results show that reconstituted, lyophilized RBCs maintained levels of ATP, 2,3 DPG, lactate, and cellular properties that are equal to or better than those in control nonlyophilized RBCs stored for a comparable period in CPDA-1. Reconstituted, lyophilized RBCs stored at 4 degrees C after rehydration also show better maintenance of ATP, 2,3 DPG, and lactate than do control RBCs stored in the same preservative solutions for comparable periods.
Sowemimo-Coker
S O
SO
Department of Basic Blood Research, Cryopharm Corporation, Pasadena, California.
Goodrich
R P
RP
Zerez
C R
CR
Tanaka
K R
KR
D019794:2,3-Diphosphoglycerate; D000225:Adenine; D000244:Adenosine Diphosphate / Q000097:blood; D000249:Adenosine Monophosphate / Q000097:blood; D000255:Adenosine Triphosphate / Q000097:blood; D001793:Blood Preservation / Q000379:methods*; D002951:Citrates; D003080:Cold Temperature; D003451:Cryoprotective Agents; D004163:Diphosphoglyceric Acids / Q000097:blood; D004907:Erythrocyte Deformability; D004909:Erythrocyte Indices; D004912:Erythrocytes / Q000737:chemistry / Q000166:cytology*; D005612:Freeze Drying; D005947:Glucose; D006801:Humans; D007773:Lactates / Q000097:blood; D008353:Mannitol; D009996:Osmotic Fragility; D010710:Phosphates; D015532:Rehydration Solutions; D012965:Sodium Chloride; D012996:Solutions
C037839:ADSOL; C034876:CPDA solutions; D002951:Citrates; D003451:Cryoprotective Agents; D004163:Diphosphoglyceric Acids; D007773:Lactates; D010710:Phosphates; D015532:Rehydration Solutions; D012996:Solutions; D019794:2,3-Diphosphoglycerate; D008353:Mannitol; D000249:Adenosine Monophosphate; D012965:Sodium Chloride; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D005947:Glucose; D000225:Adenine