Molecular cloning and expression of cDNA for mammalian translation initiation factor 5.
Eukaryotic translation initiation factor 5 (eIF-5) catalyzes the hydrolysis of GTP bound to the 40S ribosomal initiation complex (40S.AUG.Met-tRNAf-eIF-2.GTP) with the subsequent joining of a 60S ribosomal subunit resulting in the formation of a functional 80S initiation complex. A rat cDNA that encodes eIF-5 has been isolated and expressed in Escherichia coli to yield a catalytically active eIF-5 protein. The 3.55-kb cDNA encodes a protein of 429 amino acids (calculated M(r) 48,926) with properties that are similar to eIF-5 isolated from rabbit reticulocyte lysates. The deduced amino acid sequence of eIF-5 contains sequence motifs characteristic of proteins of the GTPase superfamily.
Das
K
K
Department of Developmental Biology and Cancer, Albert Einstein College of Medicine, Bronx, NY 10461.
Chevesich
J
J
Maitra
U
U
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods; D004247:DNA / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D040061:Eukaryotic Initiation Factor-5; D020558:GTP Phosphohydrolases / Q000235:genetics; D006160:Guanosine Triphosphate / Q000378:metabolism; D008099:Liver / Q000502:physiology; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D005810:Multigene Family; D009838:Oligodeoxyribonucleotides; D010448:Peptide Initiation Factors / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D014176:Protein Biosynthesis; D012346:RNA, Transfer, Amino Acyl / Q000378:metabolism; D012358:RNA, Transfer, Met; D011817:Rabbits; D015183:Restriction Mapping; D012156:Reticulocytes / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
D040061:Eukaryotic Initiation Factor-5; D046911:Macromolecular Substances; D009838:Oligodeoxyribonucleotides; D010448:Peptide Initiation Factors; D012346:RNA, Transfer, Amino Acyl; D012358:RNA, Transfer, Met; C024988:tRNA, formylmethionine-; D006160:Guanosine Triphosphate; D004247:DNA; D020558:GTP Phosphohydrolases