Distribution of non-class-III beta-tubulin isoforms in neuronal and non-neuronal cells.
beta-Tubulin isoforms in brain tissues and in cell lines were analyzed by high-resolution isoelectric focusing in combination with monoclonal antibodies. Post-translational modifications of brain non-class-III beta-tubulin isoforms were found in phylogenetically distant species ranging from pig to carp. Less extensive modifications were also observed in Neuro-2a, HeLa and 3T3 cells, where most acidic isoforms were glutamylated, while the basic, most abundant isoforms were not. The data suggest post-translational modification of non-class-III beta-tubulin isoforms in neuronal as well as in non-neuronal cells. Such modification might modulate interaction of tubulin with microtubule-associated proteins.
Linhartová
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I
Institute of Molecular Genetics, Czech Academy of Sciences, Praha.
Dráberová
E
E
Viklický
V
V
Dráber
P
P
D016475:3T3 Cells; D000818:Animals; D015153:Blotting, Western; D001921:Brain / Q000378:metabolism*; D002478:Cells, Cultured; D004591:Electrophoresis, Polyacrylamide Gel; D005347:Fibroblasts / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D009447:Neuroblastoma; D009474:Neurons / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D013045:Species Specificity; D014404:Tubulin / Q000378:metabolism*
D014404:Tubulin