title: Control of the effective P/O ratio of oxidative phosphorylation in liver mitochondria and hepatocytes.
abstract: The control exerted by substrate oxidation reactions, by ATP turnover and by the proton leak over the oxygen consumption rate, the phosphorylation rate, the proton leak rate and the protonmotive force (delta p) in isolated rat liver mitochondria under a range of conditions between non-phosphorylating (State 4) and maximum phosphorylation (State 3) was investigated by using the top-down approach of metabolic control analysis. The experiments were carried out with saturating concentrations of the substrates succinate, glutamate with malate, or pyruvate with malate. The distribution of control was very similar with each of the three substrates. The effective P/O ratio (i.e. not corrected for leak reactions) was also measured; it varied from zero in State 4 to 80-90% of the maximum theoretical P/O ratio in State 3. Under most conditions control over the effective P/O ratio was shared between proton leak (which had negative control) and the phosphorylating subsystem (which had roughly equal positive control); near State 4, substrate oxidation reactions also acquired some control over this ratio. In resting hepatocytes the effective P/O ratio was only 50% of its maximum theoretical value, corresponding to an effective P/O ratio of only 1.3 for complete oxidation of glucose. The effective P/O ratio for intracellular mitochondrial oxygen consumption was 64% of the maximum value. The control coefficient of the mitochondrial proton leak over the effective P/O ratio in cells was -0.34; the control coefficient of phosphorylation reactions over this ratio was 0.31 and the control coefficient of substrate oxidation reactions over the ratio was 0.03, showing how the coupling efficiency in cells can respond sensitively to agents that change the proton leak or the ATP demand, but not to those that change substrate oxidation.
authors: Department of Biochemistry, University of Cambridge, U.K.
authors: M D
authors: MD
authors: Brand
authors: M E
authors: ME
authors: Harper
authors: H C
authors: HC
authors: Taylor
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D005260:Female; D006863:Hydrogen-Ion Concentration; D008099:Liver / Q000378:metabolism*; D008314:Malonates / Q000494:pharmacology; D008930:Mitochondria, Liver / Q000378:metabolism*; D009243:NAD / Q000378:metabolism; D009550:Nigericin / Q000494:pharmacology; D010085:Oxidative Phosphorylation; D010101:Oxygen Consumption; D010710:Phosphates / Q000378:metabolism*; D010766:Phosphorylation; D011190:Potassium Cyanide / Q000494:pharmacology; D051381:Rats; D013386:Succinates / Q000378:metabolism; D019802:Succinic Acid
chemical_list: D008314:Malonates; D010710:Phosphates; D013386:Succinates; D009243:NAD; D000255:Adenosine Triphosphate; C030290:malonic acid; D019802:Succinic Acid; D011190:Potassium Cyanide; D009550:Nigericin