[Drosophila beta-heterochromatin: molecular organization and function. Cloning and molecular biological analysis of the lambda 20 DNA fragment from Drosophila melanogaster beta-heterochromatin].
To isolate the DNA sequences specific for the pericentric heterochromatin of Drosophila we used two CREST-autoimmune sera which bind in the Western-blot analysis the nuclear antigens of 30 kDa, 43 kDa and 45 kDa molecular weight. Cloning of the DNA fragments associated with these CREST-specific proteins of Drosophila resulted in obtaining 8 clones. One of them, lambda 20, hybridized mainly to the chromomcenter of polytene chromosomes. The further analysis indicated that the lambda 20 DNA might belong to the proximal beta-heterochromatin of the polytene chromosomes of D. melanogaster.
Baĭborodin
S I
SI
Baricheva
E M
EM
Bogachev
S S
SS
Borisevich
I V
IV
Strots
O V
OV
Filippova
M A
MA
Sharakhov
I V
IV
Shilov
A G
AG
D000818:Animals; D000906:Antibodies / Q000276:immunology; D002875:Chromosomes; D003001:Cloning, Molecular; D003429:Cross Reactions; D004247:DNA / Q000737:chemistry / Q000235:genetics*; D004331:Drosophila melanogaster / Q000235:genetics*; D006570:Heterochromatin; D006801:Humans; D007106:Immune Sera; D017403:In Situ Hybridization; D008854:Microscopy, Electron; D008938:Mitosis; D009687:Nuclear Proteins / Q000276:immunology / Q000648:ultrastructure
D000906:Antibodies; D006570:Heterochromatin; D007106:Immune Sera; D009687:Nuclear Proteins; D004247:DNA
beta-Geterokhromatin Drosophila: molekuliarnaia organizatsiia i funktsionirovanie. Klonirovanie i molekuliarno-biologicheskiĭ analiz lamda 20 fragmenta DNK iz beta-geterokhromatina Drosophila melanogaster.