Exchange of the cellular growth medium supplement from fetal bovine serum to Ultroser G increases the affinity of adenovirus for HeLa cells.
A comparative investigation was performed on the process of attachment of adenovirus type 2 to HeLa cells cultivated in the presence of 3.5% fetal bovine serum (FBS-cells) or 2% Ultroser G (USG-cells). The initial rates of virus attachment were markedly higher at temperatures between 10 and 35 degrees C for the virus binding to USG-cells than to FBS-cells. This was not caused by a higher amount of available virus-recognizing cellular receptor sites or cellular receptor units recognizing the viral fiber, but could be explained by a higher affinity of virions for USG-cells as compared to FBS-cells. Studies of virus attachment to cells, pretreated with neuraminidase and/or wheat germ agglutinin, suggested that the cellular receptor sites of FBS-cells were masked to a higher extent by sialic acid than the cellular receptor sites of USG-cells.
Blixt
Y
Y
Department of Microbiology, University of Lund, Sweden.
D000256:Adenoviridae / Q000254:growth & development*; D000818:Animals; D001802:Blood Substitutes / Q000494:pharmacology*; D002417:Cattle; D002448:Cell Adhesion; D002462:Cell Membrane / Q000737:chemistry / Q000382:microbiology; D003470:Culture Media; D005312:Fetal Blood; D006367:HeLa Cells; D006801:Humans; D019158:N-Acetylneuraminic Acid; D009439:Neuraminidase / Q000494:pharmacology; D009930:Organic Chemicals; D012794:Sialic Acids / Q000502:physiology; D013696:Temperature; D014776:Virus Cultivation; D014909:Wheat Germ Agglutinins / Q000494:pharmacology
D001802:Blood Substitutes; D003470:Culture Media; D009930:Organic Chemicals; D012794:Sialic Acids; C044609:Ultroser G; D014909:Wheat Germ Agglutinins; D009439:Neuraminidase; D019158:N-Acetylneuraminic Acid