Pre-irradiation of tissue culture flasks leads to diminished stem and progenitor cell production in long-term bone marrow cultures.
Empty plastic tissue culture flasks were exposed to X-irradiation doses of 0.3-10.0 Gy, prior to the establishment of long-term bone marrow cultures. During the course of a 10 week culture period, all irradiated plastic flasks exhibited a dramatic decrease in the number of both haemopoietic stem cells and myeloid progenitor cells, in the non-adherent layer, when compared with controls. This decrease was not due to a decrease in the number of non-adherent cells produced. Histological examination of non-adherent cells showed an increase in mature granulocytic cells with few blast cells. Morphologically, the adherent layers of irradiated flasks demonstrated a delay in appearance or absence of fat cell production. X-irradiation of glass tissue culture flasks had no deleterious effect.
Rooney
P
P
Department of Biology and Preclinical Medicine, University of St Andrews, Fife, UK.
Wright
E G
EG
D000273:Adipose Tissue / Q000166:cytology; D000818:Animals; D001854:Bone Marrow Cells; D002448:Cell Adhesion / Q000528:radiation effects; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D004307:Dose-Response Relationship, Radiation; D005898:Glass / Q000528:radiation effects*; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D007700:Kinetics; D051379:Mice; D010969:Plastics / Q000528:radiation effects*; D013234:Stem Cells / Q000166:cytology*; D017154:Stromal Cells / Q000166:cytology
D010969:Plastics