title: Drift of the sialyl-linkage specific recognition of the sialidase of influenza B virus isolates.
abstract: Sialyl-linkage specificity of the sialidase of influenza B viruses isolated in different years from 1940 through 1990 (B/Lee/40,B/Setagaya/3/56,B/Tokyo/7/66,B/Kagoshima/1/68, B/Gifu/2/73, B/Kanagawa/3/76, B/Ibaraki/2/85, B/Yamagata/16/88, and B/Bangkok/163/90) was studied with N-acetylneuraminyl (alpha 2-3)- and (alpha 2-6)-lactoses, GM3 gangliosides containing the same sialyl-oligosaccharide sequences as sialyllactose, and also with type I and type II lacto-series gangliosides carrying Neu5Ac alpha 2-3Gal and NeuAc alpha 2-6Gal linkages as substrates. From an examination of up to nine strains, the sialidases of all viruses preferentially hydrolyze substrates with Neu5Ac alpha 2-3Gal linkage rather than the Neu5Ac alpha 2-6Gal linkage. It was found that the sialidase activity toward Neu5Ac alpha 2-6Gal linkage relative to Neu5Ac alpha 2-3Gal linkage is increased in later strains, whether sialyllactose or ganglioside is used as the substrate. These results suggested that the sialidase of influenza B virus isolates has shown a drift in linkage specificity which correlates with the year of isolation.
authors: Department of Biochemistry, University of Shizuoka School of Pharmaceutical Science.
authors: G
authors: G
authors: Xu
authors: T
authors: T
authors: Suzuki
authors: G
authors: G
authors: Hanagata
authors: E
authors: E
authors: Deya
authors: M
authors: M
authors: Kiso
authors: A
authors: A
authors: Hasegawa
authors: Y
authors: Y
authors: Suzuki
mesh_terms: D000818:Animals; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002642:Chick Embryo; D005732:Gangliosides / Q000737:chemistry / Q000378:metabolism*; D009981:Influenza B virus / Q000201:enzymology*; D007785:Lactose / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D008969:Molecular Sequence Data; D009439:Neuraminidase / Q000378:metabolism*; D012794:Sialic Acids / Q000737:chemistry / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D005732:Gangliosides; D012794:Sialic Acids; C000020:N-acetylneuraminoyllactose; D009439:Neuraminidase; D007785:Lactose