Behaviour of leukocyte membrane fluidity in presence of anaesthetic drugs. Comparison between allergic patients and control subjects.
1. In this study we compared the effects of two anaesthetic drugs on the leukocyte membrane fluidity in allergic patients versus control subjects. 2. Fluidity was assessed by means of the fluorescence polarization technique. 3. We report that the treatment of the whole leukocytes with thiopental or pancuronium enhanced membrane fluidity in the allergic group as well as in the control one, but the effect was more pronounced in allergics. 4. This finding suggests a different biophysical behaviour of the leukocyte membrane towards anaesthetic drugs in allergic diseases. 5. This agrees with the hypothesis of the existence of an intrinsic abnormality in allergic cells, expressed as an initial hyperreactive state.
Aloui
R
R
Laboratoire d'Immuno-allergologie, Service de Pneumologie bât., Pierre Bénite, France.
Gallet
H
H
Biot
N
N
Perrin-Fayolle
M
M
Lagarde
M
M
Pacheco
Y
Y
D000328:Adult; D005260:Female; D005454:Fluorescence Polarization; D006801:Humans; D006967:Hypersensitivity / Q000097:blood*; D007962:Leukocytes / Q000187:drug effects* / Q000502:physiology; D008297:Male; D008560:Membrane Fluidity / Q000187:drug effects*; D008875:Middle Aged; D010197:Pancuronium / Q000494:pharmacology*; D013874:Thiopental / Q000494:pharmacology*
D010197:Pancuronium; D013874:Thiopental