Determination of amphetamine, methamphetamine and desmethyldeprenyl in human plasma by gas chromatography/negative ion chemical ionization mass spectrometry.
A sensitive and specific assay for the quantitative determination of amphetamine, methamphetamine and desmethyldeprenyl in human plasma specimens is described. Electron capture/negative ion chemical ionization gas chromatography/mass spectrometry is used to determine the extracted plasma concentrations of the three target compounds as their N-heptafluorobutyryl derivatives. Quantitation is performed by stable isotope dilution using d6-amphetamine and d6-methamphetamine as internal standards. Selected ion monitoring of the [M-HF]- ions of both the analytes and internal standards results in minimum quantifiable limits of 0.10 ng ml-1 for both amphetamine and methamphetamine and 0.25 ng ml-1 for desmethyldeprenyl. Excellent linearity (r = 0.998) up to at least 5.00 ng ml-1 is demonstrated.
Reimer
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ML
Biomedical Mass Spectrometry Unit, McGill University, Montreal, Quebec, Canada.
Mamer
O A
OA
Zavitsanos
A P
AP
Siddiqui
A W
AW
Dadgar
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D
D000661:Amphetamine / Q000097:blood*; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D008694:Methamphetamine / Q000097:blood*; D011786:Quality Control; D012044:Regression Analysis; D012642:Selegiline / Q000031:analogs & derivatives / Q000097:blood*
D012642:Selegiline; D008694:Methamphetamine; D000661:Amphetamine