title: 1,3,5-Trihydroxybenzene biodegradation by Rhodococcus sp. BPG-8.
abstract: The metabolic pathway used by Rhodococcus sp. BPG-8 isolated from oil-rich soil in Newfoundland for the degradation of 1,3,5-trihydroxybenzene (phloroglucinol) as a sole source of carbon and energy was determined. Culture filtrates of cells grown on phloroglucinol detected 1,2,3,5-tetrahydroxybenzene when extracted and analyzed using gas chromatography--mass spectrometry, thin-layer chromatography, and ultraviolet spectrophotometry. Nonaromatics were either derivatized with 2,4-dinitrophenylhydrazine and compared against authentic standards by the above methods, or detected by chemical and enzymatic methods. Extracts of cells grown on phloroglucinol contained phloroglucinol hydroxylase activity, and a dioxygenase that carried out ortho-cleavage of 1,2,3,5-tetrahydroxybenzene. The extract also showed inducible activity for further metabolism of acetopyruvate leading to accumulation of formate in the supernatant. A tentative degradative pathway for phloroglucinol by Rhodococcus sp. BPG--8 is proposed.
authors: Department of Biology and Biochemistry, Memorial University, St. John's Nfld., Canada.
authors: S
authors: S
authors: Armstrong
authors: T R
authors: TR
authors: Patel
mesh_terms: D001673:Biodegradation, Environmental; D005561:Formates / Q000378:metabolism; D006899:Mixed Function Oxygenases / Q000378:metabolism; D010101:Oxygen Consumption; D010696:Phloroglucinol / Q000737:chemistry / Q000378:metabolism*; D011773:Pyruvates / Q000378:metabolism; D012240:Rhodococcus / Q000302:isolation & purification / Q000378:metabolism*; D012988:Soil Microbiology; D013056:Spectrophotometry, Ultraviolet
chemical_list: D005561:Formates; D011773:Pyruvates; C030544:formic acid; C007691:acetylpyruvic acid; D010696:Phloroglucinol; D006899:Mixed Function Oxygenases