Experimental cancer chemotherapy using a liver metastatic model of human colon cancer transplanted into the spleen of severe combined immunodeficient mice.
We have developed a liver metastatic model of human colon cancer using severe combined immunodeficient (SCID) mice. Liver metastases were observed in all the SCID mice on day 28 after intrasplenic injection with 5 x 10(6) dissociated tumor cells of COL-2-JCK, a human colon cancer strain serially transplanted in nude mice. When this model was applied for chemotherapeutic experiments, 5-fluorouracil (5-FU) demonstrated significant antitumor effects in preventing liver metastases, whereas the efficacy of 5-FU was limited in the currently used sc-ip chemosensitivity assay in nude mice. When the human LDH-5 isozyme was evaluated in the homogenized metastatic liver tissue of SCID mice, a good correlation was obtained between the liver tumor weights and LDH-5 isozyme, suggesting that it could be a promising quantitative indicator for metastases. This model would be useful for further studies on the treatment of liver metastases of colon cancer.
Kuo
T H
TH
Department of Surgery, School of Medicine, Keio University, Tokyo, Japan.
Kubota
T
T
Nishibori
H
H
Watanabe
M
M
Furukawa
T
T
Kase
S
S
Tanino
H
H
Saikawa
Y
Y
Teramoto
T
T
Kitajima
M
M
D000818:Animals; D003110:Colonic Neoplasms / Q000473:pathology*; D005472:Fluorouracil / Q000627:therapeutic use*; D006801:Humans; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008099:Liver / Q000201:enzymology; D008113:Liver Neoplasms / Q000188:drug therapy* / Q000201:enzymology / Q000556:secondary*; D008297:Male; D051379:Mice; D016513:Mice, SCID; D009368:Neoplasm Transplantation; D013154:Spleen
D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase; D005472:Fluorouracil