title: Characterization of an aromatic amino acid-dependent Listeria monocytogenes mutant: attenuation, persistence, and ability to induce protective immunity in mice.
abstract: A transposon insertion mutant of Listeria monocytogenes was shown to be deficient in prephenate dehydratase, an enzyme acting late in the pathway for biosynthesis of phenylalanine. This mutant had reduced virulence in mice. The mutant and parent strains persisted to the same extent in the tissues of infected mice and elicited similar degrees of splenomegaly. Mice vaccinated with the mutant were protected significantly from subsequent challenge with virulent L. monocytogenes.
authors: Department of Microbiology, University of Leicester, United Kingdom.
authors: J E
authors: JE
authors: Alexander
authors: P W
authors: PW
authors: Andrew
authors: D
authors: D
authors: Jones
authors: I S
authors: IS
authors: Roberts
mesh_terms: D000818:Animals; D001428:Bacterial Vaccines / Q000276:immunology*; D015139:Blotting, Southern; D004269:DNA, Bacterial / Q000235:genetics; D005260:Female; D008089:Listeria monocytogenes / Q000235:genetics / Q000276:immunology*; D008088:Listeriosis / Q000517:prevention & control; D051379:Mice; D016254:Mutagenesis, Insertional; D014611:Vaccination; D014613:Vaccines, Attenuated / Q000276:immunology*
chemical_list: D001428:Bacterial Vaccines; D004269:DNA, Bacterial; D014613:Vaccines, Attenuated