Equine protozoal myeloencephalitis: antigen analysis of cultured Sarcocystis neurona merozoites.
Antigens of cultured Sarcocystis neurona merozoites were examined using immunoblot analysis. Blotted proteins were probed with S. cruzi, S. muris, and S. neurona antisera produced in rabbits, S. fayeri (pre- and post-infection) and S. neurona (pre- and post-inoculation) sera produced in horses, immune sera from 7 histologically confirmed cases of equine protozoal myeloencephalitis (EPM), and pre-suckle serum from a newborn foal. Eight proteins, 70, 24, 23.5, 22.5, 13, 11, 10.5, and 10 Kd, were detected only by S. neurona antiserum and/or immune serum from EPM-affected horses. Equine sera were titered by the indirect immunofluorescent antibody (IFA) method using air-dried, cultured S. neurona merozoites. Anti-Sarcocystis IFA titers were found in horses with or without EPM. Serum titers did not correspond to the number of specific bands recognized on immunoblots.
Granstrom
D E
DE
Department of Veterinary Science, University of Kentucky, Lexington 40546-0099.
Dubey
J P
JP
Davis
S W
SW
Fayer
R
R
Fox
J C
JC
Poonacha
K B
KB
Giles
R C
RC
Comer
P F
PF
D000818:Animals; D000953:Antigens, Protozoan / Q000032:analysis* / Q000302:isolation & purification; D002417:Cattle; D002478:Cells, Cultured; D004591:Electrophoresis, Polyacrylamide Gel; D006734:Horse Diseases; D006736:Horses; D015151:Immunoblotting; D008970:Molecular Weight; D012522:Sarcocystis / Q000276:immunology* / Q000302:isolation & purification; D012523:Sarcocystosis / Q000662:veterinary*
D000953:Antigens, Protozoan