Rapid and sensitive pre-column extraction high-performance liquid chromatographic assay for propofol in biological fluids.
A completely automated high-performance liquid chromatographic system is described for the determination of the phenolic anaesthetic propofol. The method is based on pre-column extraction in a closed system allowing direct injection of biological samples without any sample pretreatment. The assay is sensitive (limit of quantification is 5 ng/ml serum), reliable (the variability within a series is 2%) and rapid (results are available after 6 min).
Altmayer
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P
Klinik für Anaesthesiologie und Intensivmedizin, Universität des Saarlandes, Homburg/Saar, Germany.
Büch
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U
Büch
H P
HP
Larsen
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R
D002851:Chromatography, High Pressure Liquid; D006801:Humans; D015742:Propofol / Q000032:analysis* / Q000097:blood / Q000652:urine; D011485:Protein Binding; D013056:Spectrophotometry, Ultraviolet
D015742:Propofol