Measurement of non-enzymic glycosylation with a radiochemical assay.
1. A radiochemical assay has been developed to measure non-enzymic glycosylation (glycation), which is free from interference from enzymic glycosylation and which can be used in batch analysis. 2. Glycated residues were isolated by affinity chromatography on mini-columns, after reduction with Na[3H]BH4 and hydrolysis. 3. The specific activity of the labelled borohydride was measured by a novel procedure. 4. The method was used to measure glycation of albumin, immunoglobulin G and fibrinogen isolated from diabetic and non-diabetic subjects.
Sheikh
M A
MA
Department of Bioscience and Biotechnology, University of Strathclyde, Glasgow, U.K.
Robb
D A
DA
D001894:Borohydrides; D002846:Chromatography, Affinity; D003920:Diabetes Mellitus / Q000097:blood; D004591:Electrophoresis, Polyacrylamide Gel; D005340:Fibrinogen / Q000378:metabolism; D006023:Glycoproteins / Q000097:blood*; D006031:Glycosylation; D006801:Humans; D006868:Hydrolysis; D007074:Immunoglobulin G / Q000097:blood; D009243:NAD / Q000378:metabolism; D010084:Oxidation-Reduction; D011854:Radiochemistry; D012709:Serum Albumin / Q000378:metabolism
D001894:Borohydrides; D006023:Glycoproteins; D007074:Immunoglobulin G; D012709:Serum Albumin; D009243:NAD; C025364:sodium borohydride; D005340:Fibrinogen