Enzyme complex amplification--a signal amplification method for use in enzyme immunoassays.
An amplification system for enzyme immunoassays, in which complexes of streptavidin, biotinylated horseradish peroxidase, antibody are used to detect biotinylated target molecules was developed. In enzyme-linked immunosorbent assay (ELISA) experiments this enzyme complex enhancement (ECA) system gave up to a better than two orders of magnitude increase in sensitivity, compared to a horseradish peroxidase-conjugated streptavidin detection system. The ECA system was shown to be applicable to direct detection of biotinylated antigens, as well as in ELISA employing biotinylated antibodies and in sandwich ELISA. The ECA system was also applicable to immunoblots, giving a sensitivity comparable to that obtained using a radiochemical detection system.
Wilson
M R
MR
Department of Biochemistry, University of Sydney, NSW, Australia.
Easterbrook-Smith
S B
SB
D001426:Bacterial Proteins; D001710:Biotin; D004797:Enzyme-Linked Immunosorbent Assay; D006735:Horseradish Peroxidase; D015151:Immunoblotting; D007124:Immunoenzyme Techniques; D008722:Methods; D012680:Sensitivity and Specificity; D019809:Streptavidin
D001426:Bacterial Proteins; D001710:Biotin; D019809:Streptavidin; D006735:Horseradish Peroxidase