Lipids in the broodsac of Leucochloridium variae (Digenea, Leucochloridiidae) and its snail host Succinea ovalis.
Thin-layer chromatographic analysis was used to examine lipophilic pigments and neutral lipids in the broodsac of Leucochloridium variae and in the tissues of its snail host, Succinea ovalis. Beta-carotene and lutein were not detected in either the parasite or the host on a C-18 reversed phase layer developed in a solvent system of petroleum ether-acetonitrile-methanol (2:4:4). This chromatographic system was able to detect 10 ng of a beta-carotene standard and 100 ng of a lutein standard. The Mangold solvent system on a silica gel plate showed the presence of triacylglycerols, free sterols, and sterol esters as the major neutral lipids in both snail and parasite tissues. As seen in a previous sporocyst-snail relationship, the qualitative neutral lipid profiles of both host and parasite are similar.
Fried
B
B
Department of Biology, Lafayette College, Easton, PA 18042.
Beers
K
K
Lewis
P D
PD
D000818:Animals; D008055:Lipids / Q000032:analysis*; D010860:Pigments, Biological / Q000032:analysis; D012098:Reproduction; D012908:Snails / Q000737:chemistry* / Q000469:parasitology*; D014200:Trematoda / Q000737:chemistry*
D008055:Lipids; D010860:Pigments, Biological