title: [Acute toxoplasmosis. Laboratory diagnosis].
abstract: BACKGROUND
  The microbiological diagnosis of acute toxoplasmosis in immunosuppressed patients, cannot, usually, be done through serological techniques. The methods for isolation or direct detection of parasite are the only ones that might be helpful to determine an earlier diagnosis, on these patients.
  
  
  RESULTS
  The use of urine in 3 patients with acute toxoplasmosis, allowed us to isolate the parasite after inoculation of the sample in human embryonic fibroblast cell line (MRC-5).
  
  
  CONCLUSIONS
  In our opinion urine could be an adequate sample for isolation or detection of Toxoplasma gondii in immunosuppressed patients, and an alternative to serological methods, as well.
authors: Servicio de Análisis Clínicos-Microbiología, Hospital Arnau de Vilanova, Lérida.
authors: A
authors: A
authors: Nogués
authors: M
authors: M
authors: García
authors: E
authors: E
authors: Ribelles
authors: C
authors: C
authors: Rivas
mesh_terms: D017088:AIDS-Related Opportunistic Infections / Q000175:diagnosis / Q000469:parasitology / Q000652:urine; D000208:Acute Disease; D000328:Adult; D000818:Animals; D000953:Antigens, Protozoan / Q000652:urine; D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005347:Fibroblasts / Q000469:parasitology; D006801:Humans; D016867:Immunocompromised Host; D008297:Male; D014122:Toxoplasma / Q000276:immunology / Q000302:isolation & purification*; D014123:Toxoplasmosis / Q000175:diagnosis* / Q000469:parasitology / Q000652:urine; D014556:Urine / Q000469:parasitology
chemical_list: D000953:Antigens, Protozoan
vernacular_title: Toxoplasmosis aguda. Diagnóstico de laboratorio.