Interactions of chlorhexidine with salivary films adsorbed at solid/liquid and air/liquid interfaces.
Chlorhexidine is a cationic compound which has been shown to bind to salivary proteins and enamel through electrostatic interactions. The aims of this study were to investigate the interaction of chlorhexidine molecules with salivary films adsorbed on solid surfaces with varying physico-chemical characteristics and to investigate the effect of different concentrations of chlorhexidine on the surface tension of saliva. The interactions between 0.2% chlorhexidine digluconate with films adsorbed from whole saliva were monitored by a Rudolph Thin-film ellipsometer equipped with a He-Ne laser (632.8 nm). The films were adsorbed on hydrophilic silica surfaces which were plasma cleaned or on methylated hydrophobic surfaces. Experiments of chlorhexidine adsorption on bare surfaces were also performed. The surface tension of mixtures of whole saliva with various concentrations of (0.1%, 0.2%, 1%) chlorhexidine was monitored with a tensiometer. The results show that chlorhexidine adsorbs on both types of studied substrates. Addition of the substance followed by rinsing caused a partial desorption of the adsorbed pellicles. Furthermore, at all studied concentrations chlorhexidine reduced the interfacial tension. There are indications that the amphiphilic characteristics of the molecule play an important role in the retention of the substance in the oral cavity.
Freitas
L B
LB
Department of Periodontology, Lund University, Sweden.
Vassilakos
N
N
Arnebrant
T
T
D000327:Adsorption; D000388:Air; D001718:Birefringence; D002710:Chlorhexidine / Q000031:analogs & derivatives* / Q000493:pharmacokinetics / Q000494:pharmacology; D006801:Humans; D012463:Saliva / Q000187:drug effects / Q000378:metabolism*; D012471:Salivary Proteins and Peptides / Q000378:metabolism; D012825:Silicon; D013499:Surface Properties; D013500:Surface Tension / Q000187:drug effects; D014867:Water
D012471:Salivary Proteins and Peptides; D014867:Water; C010882:chlorhexidine gluconate; D002710:Chlorhexidine; D012825:Silicon