A pathogen-induced gene of barley encodes a HSP90 homologue showing striking similarity to vertebrate forms resident in the endoplasmic reticulum.
The full-length nucleotide sequence of a barley (Hordeum vulgare L.) leaf mRNA, found to increase rapidly in amount during infection attempts by the powdery mildew fungus (Erysiphe graminis DC. ex Mérat), is reported. The mRNA encodes a polypeptide of 809 amino acid residues which, by sequence comparison, was identified as a member of the 90 kDa heat shock protein (HSP90) family. The encoded protein most resembles the endoplasmic reticulum (ER) resident HSP90 protein, the 94 kDa glucose-regulated protein (GRP94) of vertebrates, as it possesses both the characteristic N-terminal domain including a signal peptide sequence and the C-terminal ER retention signal (Lys-Asp-Glu-Leu). A transcript cross-hybridizing at high stringency accumulated rapidly in leaves upon heat shock treatment. Genomic DNA blot analysis indicated the presence of a family of related genes in the barley genome.
Walther-Larsen
H
H
Department of Plant Biology, Royal Veterinary and Agricultural University, Frederiksberg, Copenhagen, Denmark.
Brandt
J
J
Collinge
D B
DB
Thordal-Christensen
H
H
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D004247:DNA; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D017343:Genes, Plant; D006360:Heat-Shock Proteins / Q000235:genetics*; D001467:Hordeum / Q000235:genetics* / Q000382:microbiology; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics*; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic; D014714:Vertebrates / Q000235:genetics
D006360:Heat-Shock Proteins; D010940:Plant Proteins; D004247:DNA