[Regulation of cholesterol absorption in human and rat small intestine epithelial cells].
Cholesterol absorption in human small intestine organ culture and rat small intestine epithelial cell culture IRD-98 has been studied using [14C] cholesterol, [3H] cholesterol and [14C] sitosterol. It has been found that cholesterol absorption is a dose- and time-dependent process, while sitosterol absorption is not and makes up to about 25% of the total cholesterol absorption. Cholesterol absorption appeared to be a specific process. The endocytosis inhibitor monensin decreased specific cholesterol absorption by 37%. Cholesterol absorption was examined under different conditions influencing cholesterol metabolism in the cell. Loading of IRD-98 cells with non-lipoprotein cholesterol caused a dose-dependent decrease of cholesterol absorption. The inhibitor of acyl coenzyme A:cholesterol acyl transferase (ACAT), compound Sandoz 58-035, had a similar effect on cholesterol absorption. Lovastatin, an inhibitor of 3-hydroxymethyl-3-glutaryl coenzyme A (HMG-CoA) reductase, stimulated cholesterol absorption in a dose-dependent manner. Loading of cells with cholesterol, lovastatin and Sandoz 58-035 had no effect on sitosterol absorption. The possibility has been demonstrated of using human small intestine organ culture and rat small intestine epithelial cell culture IRD-98 as models for studying cholesterol absorption.
Safonova
I G
IG
Laboratory of Gastroenterology and Nutrition, Faculty of Medicine, Nice University, France.
Sviridov
D D
DD
Rampal'
P
P
Nano
Zh L
ZhL
Pavlov
M Iu
MIu
Repin
V S
VS
D000293:Adolescent; D000818:Animals; D002478:Cells, Cultured; D002648:Child; D002675:Child, Preschool; D002784:Cholesterol / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism / Q000502:physiology; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D007408:Intestinal Absorption / Q000187:drug effects; D007421:Intestine, Small / Q000166:cytology / Q000378:metabolism* / Q000502:physiology; D009924:Organ Culture Techniques; D051381:Rats; D012855:Sitosterols / Q000378:metabolism
D004791:Enzyme Inhibitors; D012855:Sitosterols; C025473:gamma-sitosterol; D002784:Cholesterol
Reguliatsiia vsasyvaniia kholesterina v épitelial'nykh kletkakh tonkogo kishechnika cheloveka i krysy.