Polarized distribution of gamma interferon-stimulated MHC antigens and transferrin receptors in a clonal cell line isolated from Fisher rat thyroid (FRT cells).
The characteristics of a polarized epithelial cell line and dynamics of an endogenous polarized plasma membrane constituent were studied by use of an subclone, FRT-9, from the Fisher rat thyroid cell line, FRT. Transmission electron microscopy (conventional, freeze-fracture), determination of transepithelial electrical parameters and immuno-fluorescence study, were used to establish polarity and demonstrated the basolateral distribution of transferrin receptors and the major histocompatibility complex antigens (constitutive Class I or gamma interferon-induced Class II).
Boudier
J A
JA
INSERM U270, Faculté de Médecine Nord, Marseille, France.
Fantini
J
J
Gerard
C
C
Verrier
B
B
D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000737:chemistry / Q000648:ultrastructure; D002999:Clone Cells; D005455:Fluorescent Antibody Technique; D005614:Freeze Fracturing; D015395:Histocompatibility Antigens Class I / Q000032:analysis*; D000949:Histocompatibility Antigens Class II / Q000032:analysis*; D007371:Interferon-gamma / Q000494:pharmacology*; D008854:Microscopy, Electron; D051381:Rats; D011916:Rats, Inbred F344; D011990:Receptors, Transferrin / Q000032:analysis*; D013961:Thyroid Gland / Q000737:chemistry* / Q000166:cytology* / Q000648:ultrastructure
D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; D011990:Receptors, Transferrin; D007371:Interferon-gamma