The gene encoding the human corticostatin HP-4 precursor contains a recent 86-base duplication and is located on chromosome 8.
The human neutrophil-derived cationic peptide HP-4 exhibits corticostatic activity on adrenal cells and is an L-type calcium channel agonist at nanomolar concentrations. Complementary DNA clones encoding the HP-4 precursor have been isolated from a human bone marrow cDNA library by screening with oligonucleotide probes. The nucleotide sequence shares about 72% identity with the cDNA encoding defensin HP-1, but differs from it, and from other genes of this family characterized to date, by an extra 83-base segment. This extra segment is not adjacent to an intron and is apparently the result of a recent duplication within the coding region corresponding to most of the mature HP-4 peptide. The predicted amino acid sequence shows the HP-4 precursor structure to be typical of this family of molecules. By analysis of DNA from a pannel of hamster/human hybrid cell lines, the HP-4 gene was found to be on chromosome 8, as is the gene for human peptide HP-1. Comparison with the few sequences of other corticostatin/defensin genes available does not indicate distinct lineages of corticostatic and noncorticostatic peptides, since HP-1 and HP-4 cDNA sequences share more identity with each other than either shares with cDNAs encoding rabbit MCP-1 or MCP-2, or guinea pig GNCP-1.
Palfree
R G
RG
Department of Biochemistry, McGill University Montreal, Quebec, Canada.
Sadro
L C
LC
Solomon
S
S
D000595:Amino Acid Sequence; D000818:Animals; D023181:Antimicrobial Cationic Peptides; D001483:Base Sequence; D001798:Blood Proteins / Q000235:genetics*; D000090266:Chromobox Protein Homolog 5; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics; D002874:Chromosome Mapping; D002898:Chromosomes, Human, Pair 8; D023082:Defensins; D006168:Guinea Pigs; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins; D051379:Mice; D008969:Molecular Sequence Data; D005810:Multigene Family; D010455:Peptides / Q000235:genetics*; D011498:Protein Precursors / Q000235:genetics*; D011506:Proteins / Q000235:genetics; D011817:Rabbits; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D023084:alpha-Defensins
D023181:Antimicrobial Cationic Peptides; D001798:Blood Proteins; D002868:Chromosomal Proteins, Non-Histone; D023082:Defensins; D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides; D011498:Protein Precursors; D011506:Proteins; D023084:alpha-Defensins; C406413:human neutrophil peptide 1; C406428:human neutrophil peptide 4; D000090266:Chromobox Protein Homolog 5; C054598:corticostatin