Immunofluorescence of endomyocardial biopsy specimens: methods and interpretation.
On the basis of our experience with prospective observation of immunofluorescence microscopy in 5775 biopsy specimens from 335 consecutively followed heart transplant cases, we describe the immunofluorescent method in detail and the potential artifacts and method of interpretation of the findings. A strategy for use of the method on the basis of the experience of the authors is proposed.
Hammond
E H
EH
Department of Pathology, LDS Hospital, Salt Lake City, Utah 84143.
Hansen
J K
JK
Spencer
L S
LS
Jensen
A
A
Yowell
R L
RL
D003165:Complement System Proteins / Q000032:analysis; D003331:Coronary Vessels / Q000276:immunology; D004699:Endocardium / Q000378:metabolism / Q000473:pathology; D005337:Fibrin / Q000032:analysis; D005340:Fibrinogen / Q000032:analysis; D005455:Fluorescent Antibody Technique; D006084:Graft Rejection / Q000175:diagnosis*; D006684:HLA-DR Antigens / Q000032:analysis; D016027:Heart Transplantation; D006801:Humans; D007136:Immunoglobulins / Q000032:analysis; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology; D011446:Prospective Studies
D006684:HLA-DR Antigens; D007136:Immunoglobulins; D005337:Fibrin; D005340:Fibrinogen; D003165:Complement System Proteins