Transforming growth factor-beta 1 modulates calcium metabolism in Sertoli cells.
The mechanism of signal transduction by transforming growth factor-beta 1 (TGF-beta 1) is largely unknown. In this study, TGF-beta 1 was able to stimulate 45Ca2+ uptake in cultured rat Sertoli cells. In contrast to the rapid stimulation of 45Ca2+ uptake previously observed in response to FSH (< 1 min), the response to TGF-beta 1 required several hours and was abolished by actinomycin-D. Although TGF-beta 1 had no effect on basal calcium uptake before 1 h, it completely inhibited the FSH-stimulated calcium uptake in short term incubations (2 and 20 min). These effects of TGF-beta 1 on calcium metabolism may be directly related to the ability of this cytokine to regulate cell growth and differentiation in many cell systems.
Grasso
P
P
Department of Biochemistry and Molecular Biology, Albany Medical College, New York 12208.
Reichert
L E
LE
Sporn
M B
MB
Santa-Coloma
T A
TA
D000818:Animals; D001886:Bordetella pertussis; D002118:Calcium / Q000378:metabolism*; D002121:Calcium Channel Blockers / Q000494:pharmacology; D002478:Cells, Cultured; D003609:Dactinomycin / Q000494:pharmacology; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D008297:Male; D012708:Sertoli Cells / Q000187:drug effects / Q000378:metabolism*; D016212:Transforming Growth Factor beta / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D010566:Virulence Factors, Bordetella / Q000494:pharmacology
D002121:Calcium Channel Blockers; D016212:Transforming Growth Factor beta; D010566:Virulence Factors, Bordetella; D003609:Dactinomycin; D005640:Follicle Stimulating Hormone; D002118:Calcium