Isolation of sympathonectin; a substrate-bound protein which induces preferential growth of sympathetic fibers in vitro.
A protein named 'sympathonectin', was purified from chick heart cell-conditioned medium (HCM), on the basis of its ability to direct the neurite outgrowth of cultured sympathetic neurons. The most purified fraction showed a doublet band on SDS-PAGE with an apparent molecular weight of 370 kDa. The biological activity of sympathonectin was over 100 times higher than that of laminin. Immunoblotting with anti-sympathonectin of the 100,000 x g pellet (particulate) fraction of HCM identified a distinct 370 kDa band; this molecule did not react to the anti-laminin serum. The immunohistochemical analysis showed that the antibody against sympathonectin stained heart tissue but not skeletal muscle tissue, whereas anti-laminin serum stained both tissues. These results suggest that sympathonectin may play a role during sympathetic innervation of target organs.
Kuromi
H
H
Department of Neuropharmacology, School of Medicine, Chiba University, Japan.
D000818:Animals; D002642:Chick Embryo; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D017077:Culture Media, Conditioned; D046508:Culture Techniques; D004305:Dose-Response Relationship, Drug; D005455:Fluorescent Antibody Technique; D015151:Immunoblotting; D007797:Laminin / Q000378:metabolism; D009412:Nerve Fibers / Q000502:physiology*; D009479:Neuropeptides / Q000302:isolation & purification* / Q000494:pharmacology*; D013564:Sympathetic Nervous System / Q000254:growth & development*; D014018:Tissue Distribution
D017077:Culture Media, Conditioned; D007797:Laminin; D009479:Neuropeptides