Antibody-magnetite method for selective concentration of Giardia lamblia cysts from water samples.
An antibody-magnetite method was developed in order to selectively concentrate Giardia cysts from water samples. The indirect technique employed a mouse immunoglobulin G anti-Giardia antibody as a primary antibody and an anti-mouse immunoglobulin G antibody-coated magnetite particle as a secondary labeling reagent. The magnetically labeled cysts were then concentrated by high-gradient magnetic separation. Ninety percent of the seeded cysts were recovered from buffer when this method was employed. An average of 82% of the seeded cysts were recovered from water samples with various turbidities. Significantly higher cyst recoveries were obtained from water samples with turbidities below 600 nephelometric turbidity units.
Bifulco
J M
JM
Environmental Monitoring Systems Laboratory, U.S. Environmental Protection Agency, Cincinnati, Ohio 45268.
Schaefer
F W
FW
D000372:Agglutination Tests; D000818:Animals; D000911:Antibodies, Monoclonal; D000913:Antibodies, Protozoan / Q000276:immunology*; D004305:Dose-Response Relationship, Drug; D052203:Ferrosoferric Oxide; D016829:Giardia lamblia / Q000302:isolation & purification*; D007501:Iron; D008280:Magnetics; D008863:Microspheres; D010087:Oxides; D013205:Staphylococcal Protein A; D014867:Water
D000911:Antibodies, Monoclonal; D000913:Antibodies, Protozoan; D010087:Oxides; D013205:Staphylococcal Protein A; D014867:Water; D007501:Iron; D052203:Ferrosoferric Oxide