title: Insulin enhances the growth of cartilage in organ and tissue cultures of mouse neonatal mandibular condyle.
abstract: Condylar cartilages were cultured in the form of organ cultures on top of collagen sponges in medium containing 2% fetal calf serum and were treated with 3.5-350 nM insulin for 6 days. Doses of 175 nM of insulin caused a marked increase (+96%) in DNA synthesis and in proteoglycan production (+74%), features that manifested themselves structurally by a 60% increase in overall size of the cultured explants. Using a tissue culture system comprised of cartilage progenitor cells, insulin was found to enhance the differentiation of the progenitor cells so that by 6 days in culture an appreciable nodule of differentiated chondrocytes developed. The latter was surrounded by perichondrial cells whereas the extracellular matrix within the newly formed, insulin-induced, nodule reacted positively for cartilage-specific antigens (type II collagen and bone sialoprotein). It is suggested that insulin induces a direct stimulatory effect on progenitor cell proliferation, cartilage differentiation, and extracellular matrix deposition.
authors: Laboratory for Musculoskeletal Research, Bruce Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, Haifa.
authors: G
authors: G
authors: Maor
authors: M
authors: M
authors: Silbermann
authors: K
authors: K
authors: von der Mark
authors: D
authors: D
authors: Heingard
authors: Z
authors: Z
authors: Laron
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001345:Autoradiography; D002356:Cartilage / Q000166:cytology / Q000187:drug effects* / Q000254:growth & development; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D046508:Culture Techniques; D004247:DNA / Q000096:biosynthesis; D007328:Insulin / Q000494:pharmacology*; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology; D008335:Mandibular Condyle / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D008856:Microscopy, Fluorescence; D009924:Organ Culture Techniques; D011509:Proteoglycans / Q000096:biosynthesis; D017526:Receptor, IGF Type 1 / Q000502:physiology
chemical_list: D007328:Insulin; D011509:Proteoglycans; D007334:Insulin-Like Growth Factor I; D004247:DNA; D017526:Receptor, IGF Type 1