title: Serum chemistry and lipid profiles in neonatal beagle puppies fed homemade milk replacer formulas.
abstract: Milk replacer formulas based on cow's milk and egg yolks are frequently recommended for use in neonatal puppies. These formulas are lower in protein, kilocalories, calcium, and phosphorus than bitch's milk. In addition, the cholesterol content is greater than bitch's milk. The effect of feeding these formulas on serum chemistry profiles, lipid profiles, and alkaline phosphatase isoenzyme profiles of 5-week-old beagle puppies was studied. Three groups of beagle puppies were fed bitch's milk (control) (n = 18), a homemade milk-egg-oil formula (Formula 1) (n = 18), or a homemade milk-egg-oil formula supplemented with additional calcium and phosphorous (Formula 2) (n = 18). Concentrations of serum urea nitrogen, albumin, and total CO2 were lower (P < 0.05), and concentrations of serum phosphorus, globulins, sodium, chloride, and cholesterol were higher (P < 0.05) in formula-fed puppies than bitch-fed puppies. Serum potassium concentration was lower in the puppies fed Formula 1 than in the control puppies (P < 0.05), and serum potassium concentration in the puppies fed Formula 2 was not significantly different from that in puppies fed Formula 1 or the control puppies. Total triglyceride (TG) and high density lipoprotein2 cholesterol (HDL2) concentrations were similar in all three groups of puppies but the combined high density lipoprotein1 (HDL1) plus low density lipoprotein (LDL) cholesterol fraction was higher (P < 0.05) in the formula-fed puppies and accounted for the majority of the increase in cholesterol. There were no differences (P < 0.05) in total serum alkaline phosphatase (ALP) or bone-derived ALP (BALP) concentrations among the groups, however there was a higher (P < 0.05) serum concentration of liver-derived ALP (LALP) in the Formula 1-fed puppies. Feeding homemade egg and cow's milk-based puppy replacement formulas is not recommended for long term use.
authors: Department of Clinical Sciences, College of Veterinary Medicine, Fort Collins 80523.
authors: M L
authors: ML
authors: Chandler
authors: E
authors: E
authors: Miller
authors: P N
authors: PN
authors: Olson
authors: S L
authors: SL
authors: Ralston
mesh_terms: D000469:Alkaline Phosphatase / Q000097:blood*; D000818:Animals; D000831:Animals, Newborn / Q000097:blood*; D001774:Blood Chemical Analysis; D001903:Bottle Feeding / Q000662:veterinary*; D004285:Dogs / Q000097:blood*; D004531:Eggs; D007527:Isoenzymes / Q000097:blood*; D008055:Lipids / Q000097:blood*; D008892:Milk
chemical_list: D007527:Isoenzymes; D008055:Lipids; D000469:Alkaline Phosphatase