Molecular cloning of rat cytokine synthesis inhibitory factor (IL-10) cDNA and expression in spleen and macrophages.
Rat cytokine synthesis inhibitory factor (IL-10) was cloned. Like mouse and human IL-10 (mIL-10, hIL-10), rat IL-10 exhibits strong DNA and amino acid sequence homology to the open reading frame in the Epstein-Barr virus, BCRFI. The supernatant of COS-7 transfectants with rat IL-10 was found to inhibit the production of IFN-gamma by murine Th1 cells. A probe from the rat IL-10 was used in an RNase protection assay to demonstrate that IL-10 was expressed in LPS-stimulated rat spleen and FACS-purified OX-42-reactive peritoneal macrophages. The production of IL-10 by macrophages, as shown in the current study, suggests that IL-10 may have an autocrine function in inflammation.
Feng
L
L
Department of Immunology, Scripps Research Institute, La Jolla, CA 92037.
Tang
W W
WW
Chang
J C
JC
Wilson
C B
CB
D000595:Amino Acid Sequence; D000818:Animals; D002478:Cells, Cultured; D003001:Cloning, Molecular; D004247:DNA; D015951:Factor Xa / Q000378:metabolism; D006801:Humans; D016753:Interleukin-10 / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008070:Lipopolysaccharides; D008264:Macrophages / Q000378:metabolism*; D008969:Molecular Sequence Data; D010529:Peritoneal Cavity / Q000166:cytology; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013154:Spleen / Q000378:metabolism*
D008070:Lipopolysaccharides; D011993:Recombinant Fusion Proteins; D016753:Interleukin-10; D004247:DNA; D015951:Factor Xa