Epidermal growth factor and isoproterenol stimulation of the Ras-guanine nucleotide exchange factor in proliferating rat parotid gland acinar cells.
Chronic treatment of rat parotid gland acinar cells with either epidermal growth factor (EGF) or the beta-adrenergic receptor agonist isoproterenol leads to cell proliferation through activation of the tyrosine kinase second messenger signalling pathway. Activation of p21ras activity in acinar cells was evaluated by measuring the levels of protein bound GTP and GDP. Both EGF and isoproterenol increased the amount of p21ras-GTP complex during active proliferation. The increase in bound GTP appears to be the result of an increased activity for the Ras-guanine nucleotide exchange factor.
Wang
P L
PL
Department of Oral Biology, University of Florida, Gainesville 32610.
Nakagawa
Y
Y
Humphreys-Beher
M G
MG
D000818:Animals; D002455:Cell Division; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D015852:Eukaryotic Initiation Factor-2 / Q000378:metabolism; D005260:Female; D020662:Guanine Nucleotide Exchange Factors; D006153:Guanosine Diphosphate / Q000378:metabolism; D006160:Guanosine Triphosphate / Q000378:metabolism; D007545:Isoproterenol / Q000494:pharmacology*; D015689:Oncogene Protein p21(ras) / Q000378:metabolism*; D010306:Parotid Gland / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D011506:Proteins / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction; D020663:ras Guanine Nucleotide Exchange Factors
D015852:Eukaryotic Initiation Factor-2; D020662:Guanine Nucleotide Exchange Factors; D011506:Proteins; D020663:ras Guanine Nucleotide Exchange Factors; D006153:Guanosine Diphosphate; D004815:Epidermal Growth Factor; D006160:Guanosine Triphosphate; D015689:Oncogene Protein p21(ras); D007545:Isoproterenol