title: Organization of Lrp-binding sites upstream of ilvIH in Salmonella typhimurium.
abstract: Lrp, a major regulatory protein in Escherichia coli, controls the expression of numerous operons, including ilvIH. Lrp binds to six sites upstream of ilvIH, and Lrp binding is required for ilvIH expression. We show here that an Lrp-like protein is also present in Salmonella typhimurium. This protein can bind both E. coli and S. typhimurium ilvIH DNA, as can E. coli Lrp. Methidiumpropyl-EDTA footprinting studies were performed with purified E. coli Lrp and S. typhimurium ilvIH DNA. Six binding sites were defined, three of them being similar to corresponding sites in E. coli, and three being organized differently. A consensus derived from six S. typhimurium sites is compatible with that derived from a similar analysis of E. coli sequences.
authors: Section of Genetics and Development, Cornell University, Ithaca, New York 14853.
authors: Q
authors: Q
authors: Wang
authors: M
authors: M
authors: Sacco
authors: E
authors: E
authors: Ricca
authors: C T
authors: CT
authors: Lago
authors: M
authors: M
authors: De Felice
authors: J M
authors: JM
authors: Calvo
mesh_terms: D000095:Acetolactate Synthase; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D001483:Base Sequence; D001665:Binding Sites; D016384:Consensus Sequence; D004269:DNA, Bacterial / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics; D029968:Escherichia coli Proteins; D005798:Genes, Bacterial; D051756:Leucine-Responsive Regulatory Protein; D008969:Molecular Sequence Data; D009876:Operon; D011401:Promoter Regions, Genetic; D012486:Salmonella typhimurium / Q000235:genetics*; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; C497278:Lrp protein, E coli; D014157:Transcription Factors; D051756:Leucine-Responsive Regulatory Protein; C080946:IlvI protein, Bacteria; D000095:Acetolactate Synthase; C068838:ilvH protein, E coli