Platelet-activating factor stimulates protein kinase C translocation in cerebral microvessels.
The effects of platelet-activating factor (PAF) on distribution of protein kinase C activity in brain microvessels have been examined. Our results indicate that PAF caused an increase of protein kinase C activity in membrane, accompanied by a loss of activity in the cytosol. This effect resulted to be dose-dependent and the translocation was evident after 30 min of PAF treatment. These results suggest that PAF could play a role in transport processes in the blood-brain barrier, involving protein phosphorylation by activation of protein kinase C.
Catalán
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RE
Departamento de Biología Molecular, Centro de Biología Molecular Severo Ochoa (CSIC-UAM), Universidad Autónoma de Madrid, Spain.
Martínez
A M
AM
Aragonés
M D
MD
Garde
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E
Díaz
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G
D000818:Animals; D001692:Biological Transport; D001921:Brain / Q000098:blood supply* / Q000201:enzymology; D002196:Capillaries / Q000201:enzymology*; D004789:Enzyme Activation; D066298:In Vitro Techniques; D008297:Male; D010972:Platelet Activating Factor / Q000494:pharmacology*; D011493:Protein Kinase C / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
D010972:Platelet Activating Factor; D011493:Protein Kinase C