Transfer of a foreign gene into the brain using adenovirus vectors.
The ability of a replication-deficient adenovirus vector to transfer a foreign gene into neural cells of adult rats in vivo has been analysed. A large number of neural cells (including neurons, astrocytes and ependymal cells) expressed an E. coli lacZ transgene for at least 45 days after inoculation of various brain areas. Injecting up to 3 x 10(5) pfu in 10 microliters did not result in any detectable cytopathic effects--these were only observed for very high titres of infection (> 10(7) pfu 10 microliters-1). Adenovirus vectors therefore appear to be a promising means for in vivo transfer of therapeutic genes into the central nervous system.
Akli
S
S
U 129 INSERM, Institut Cochin de Génétique Moléculaire, Paris, France.
Caillaud
C
C
Vigne
E
E
Stratford-Perricaudet
L D
LD
Poenaru
L
L
Perricaudet
M
M
Kahn
A
A
Peschanski
M R
MR
D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D001921:Brain / Q000166:cytology* / Q000201:enzymology; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D005798:Genes, Bacterial; D015316:Genetic Therapy / Q000379:methods; D005822:Genetic Vectors; D006056:Golgi Apparatus / Q000201:enzymology / Q000648:ultrastructure; D009474:Neurons / Q000166:cytology* / Q000201:enzymology; D051381:Rats; D017207:Rats, Sprague-Dawley; D011995:Recombination, Genetic; D014162:Transfection / Q000379:methods*; D001616:beta-Galactosidase / Q000032:analysis / Q000235:genetics / Q000378:metabolism*
D001616:beta-Galactosidase