Cell-surface location of Listeria-specific protein p60--detection of Listeria cells by indirect immunofluorescence.
A specific polyclonal antiserum was prepared against a gel-purified 60 kDa extracellular protein of Listeria monocytogenes ATCC 19111 corresponding to protein p60 previously detected in culture broths of L. monocytogenes strains Mackaness and EGD [Kuhn, M. & Goebel, W. (1989), Infection and Immunity 57, 55-61]. Indirect immunogold labelling combined with transmission electron microscopy and high-resolution scanning electron microscopy were used to investigate the location and distribution of p60 on the bacterial cell surface. In bacteria grown to the early stationary phase about 25% of the extracellular protein was estimated to be associated with the cell surface. The anti-p60 antiserum proved to be Listeria-specific. In an indirect immunofluorescence test the antiserum reacted with Listeria strains representing all species and different serotypes, except L. seeligeri, L. welshimeri, L. grayi and L. murrayi. No immunological cross-reactions were observed with 27 strains of bacteria from 16 other genera. The value of the anti-p60 antiserum in developing a diagnostic assay for Listeria cells in environmental samples and foods is discussed.
Ruhland
G J
GJ
Institut für Genetik und Mikrobiologie, Universität München, FRG.
Hellwig
M
M
Wanner
G
G
Fiedler
F
F
D000907:Antibodies, Bacterial; D000918:Antibody Specificity; D001426:Bacterial Proteins / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D003429:Cross Reactions; D005455:Fluorescent Antibody Technique; D015151:Immunoblotting; D008089:Listeria monocytogenes / Q000276:immunology / Q000378:metabolism* / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D016253:Microscopy, Immunoelectron; D008970:Molecular Weight
D000907:Antibodies, Bacterial; D001426:Bacterial Proteins