The two Staphylococcal bi-component toxins, leukocidin and gamma-hemolysin, share one component in common.
Staphylococcal bi-component toxins, leukocidin and gamma-hemolysin, consist of two protein components, i.e. F and S for leukocidin and H gamma I and H gamma II for gamma-hemolysin. In this study we purified H gamma I and H gamma II to homogeneity from the culture medium of Staphylococcus aureus RIMD 310925 and compared their properties with those of F and S purified from the same source. The N-terminal 59- and C-terminal 2-residue amino acid sequences, apparent molecular mass, and isoelectric point of purified H gamma I were the same as those of F. In an Ouchterlony double diffusion test a fused line without spur was formed between F and H gamma I using either anti-F or anti-H gamma I antibodies. A synergistic action of F and H gamma II caused hemolysis of human red blood cells, and H gamma I acted synergistically with S to exhibit leukocidin activity. We conclude that the two toxins share one protein component (F = H gamma I) in common and leukocidin- and gamma-hemolysin-specific activities are determined by S and H gamma II, respectively. It is also reported that the N-terminal 58-residue sequence of H gamma II is 72% similar to the corresponding sequence of S.
Kamio
Y
Y
Department of Applied Biological Chemistry, Faculty of Agriculture, Tohoku University, Sendai, Japan.
Rahman
A
A
Nariya
H
H
Ozawa
T
T
Izaki
K
K
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry; D001427:Bacterial Toxins / Q000737:chemistry*; D015153:Blotting, Western; D006460:Hemolysin Proteins / Q000737:chemistry*; D005779:Immunodiffusion; D007956:Leukocidins / Q000737:chemistry*; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D013211:Staphylococcus aureus / Q000737:chemistry*
D001426:Bacterial Proteins; D001427:Bacterial Toxins; D006460:Hemolysin Proteins; D007956:Leukocidins; D046911:Macromolecular Substances; C037956:gamma-hemolysin, Staphylococcus aureus