Inhibition of cytokine production and endothelial expression of adhesion antigens by 5'-methylthioadenosine.
We investigated the activity of endogenous nucleoside 5'-deoxy-5'-methylthioadenosine (MTA) on both the production of inflammatory cytokines and the cytokine-dependent endothelial expression of adhesion molecules. The compound inhibited the production of tumor necrosis factor (but not interleukin-1) in lipopolysaccharide-activated macrophages. In addition, MTA selectively inhibited the expression of intercellular adhesion molecule-1 in endothelial cells activated with interleukin-1. This effect was paralleled by a reduction in endothelial adhesiveness for polymorphonuclear leukocytes. These data suggest that MTA might have anti-inflammatory activity.
Cerri
M A
MA
BioResearch SpA, Liscate, Milano, Italy.
Beltrán-Nuñez
A
A
Bernasconi
S
S
Dejana
E
E
Bassi
L
L
Bazzoni
G
G
D000818:Animals; D002448:Cell Adhesion / Q000276:immunology; D015815:Cell Adhesion Molecules / Q000096:biosynthesis* / Q000276:immunology; D003583:Cytokinins / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D003839:Deoxyadenosines / Q000494:pharmacology*; D004730:Endothelium, Vascular / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D066298:In Vitro Techniques; D008264:Macrophages / Q000187:drug effects / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D009504:Neutrophils / Q000187:drug effects / Q000276:immunology; D013872:Thionucleosides / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D015815:Cell Adhesion Molecules; D003583:Cytokinins; D003839:Deoxyadenosines; D013872:Thionucleosides; D014409:Tumor Necrosis Factor-alpha; C008500:5'-methylthioadenosine