title: The mouse low density lipoprotein receptor gene: cDNA sequence and exon-intron structure.
abstract: The low density lipoprotein (LDL) receptor plays a central role in the cholesterol metabolism. The cDNA sequence of the mouse low density lipoprotein receptor (Ldlr) gene has been determined and shows 76% homology with the human gene. The exon-intron structure has been determined for the 129/J mouse strain. The gene is composed of 18 exons and spans a region of 28 kb. In addition, the promoter regions of the mouse and human genes are homologous. Northern blot analysis revealed an mRNA of approximately 5 kb. The cloning of the Ldlr gene will enhance the usefulness of the mouse for the study of cholesterol metabolism and, in particular, for carrying out gene targeting experiments.
authors: MGC-Department of Human Genetics, Leiden University, The Netherlands.
authors: M J
authors: MJ
authors: Hoffer
authors: M M
authors: MM
authors: van Eck
authors: F
authors: F
authors: Petrij
authors: A
authors: A
authors: van der Zee
authors: E
authors: E
authors: de Wit
authors: D
authors: D
authors: Meijer
authors: G
authors: G
authors: Grosveld
authors: L M
authors: LM
authors: Havekes
authors: M H
authors: MH
authors: Hofker
authors: R R
authors: RR
authors: Frants
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D015870:Gene Expression; D005796:Genes; D006801:Humans; D007438:Introns; D051379:Mice; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics; D011973:Receptors, LDL / Q000235:genetics*; D015183:Restriction Mapping; D016415:Sequence Alignment
chemical_list: D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger; D011973:Receptors, LDL; D004247:DNA