title: Sequences of genes encoding naphthalene dioxygenase in Pseudomonas putida strains G7 and NCIB 9816-4.
abstract: The multicomponent enzyme, naphthalene dioxygenase, initiates the metabolism of naphthalene by Pseudomonas putida strains G7 (PpG7) and NCIB 9816-4 (Pp9816-4). The genes involved (nahAaAbAcAd) are encoded by the NAH7 and pDTG1 plasmids, respectively, and form part of the nah operon. The locations of the structural genes were determined on previously cloned fragments of DNA. The nucleotide (nt) sequences were determined for nahAaAb from Pp9816-4 and for nahAaAbAcAd from PpG7. The appropriate open reading frames were identified using N-terminal amino acid sequences determined from the purified proteins. The two nt sequences showed 93% homology, with the least homology seen upstream from the promoter region.
authors: Amgen Inc., Amgen Center, Thousand Oaks, CA 91320-1789.
authors: M J
authors: MJ
authors: Simon
authors: T D
authors: TD
authors: Osslund
authors: R
authors: R
authors: Saunders
authors: B D
authors: BD
authors: Ensley
authors: S
authors: S
authors: Suggs
authors: A
authors: A
authors: Harcourt
authors: W C
authors: WC
authors: Suen
authors: D L
authors: DL
authors: Cruden
authors: D T
authors: DT
authors: Gibson
authors: G J
authors: GJ
authors: Zylstra
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial; D049308:Dioxygenases; D005091:Exons; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000235:genetics* / Q000378:metabolism; D010105:Oxygenases / Q000235:genetics* / Q000378:metabolism; D016958:Pseudomonas putida / Q000201:enzymology / Q000235:genetics*; D015183:Restriction Mapping
chemical_list: D004269:DNA, Bacterial; D009097:Multienzyme Complexes; D010105:Oxygenases; D049308:Dioxygenases; C038703:naphthalene dioxygenase