[Nuclear proteins, specifically binding the regulatory region of rat tryptophan oxygenase].
Using electrophoretic mobility shift assay we have analysed the binding of rat liver nuclear proteins to the fragments from -466 to -292 and from -292 to -178 relative to the transcriptional start site of the rat tryptophan oxygenase gene. Studies of the competition with a synthetic consensus sequence for the NF 1 recognition site have shown that the liver nuclear proteins, responsible for the formation of specific complexes with these fragments belong to the family of the nuclear factor 1 (NF 1). We have also found that the trans-acting factors of the NF 1 family form several complexes with each of the two tested fragments of the tryptophan oxygenase gene.
Chikhirzhina
G I
GI
Fedorova
S A
SA
Chespokov
I N
IN
Romanovskaia
E V
EV
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D008099:Liver / Q000201:enzymology; D008297:Male; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000378:metabolism*; D051381:Rats; D012045:Regulatory Sequences, Nucleic Acid; D014158:Transcription, Genetic; D014366:Tryptophan Oxygenase / Q000235:genetics*
D009687:Nuclear Proteins; D014366:Tryptophan Oxygenase
Iadernye belki, spetsificheski sviazyvaiushchiesia s reguliatornoĭ oblast'iu gena triptofanoksigenazy krysy.