Natural cycloheximide resistance in yeast. The role of ribosomal protein L41.
The yeast Kluyveromyces lactis is resistant to high concentrations (1 mg/ml) of the antibiotic cycloheximide. Using in vitro translation studies it was confirmed that this extreme resistance is a property of ribosomes. The resistance determinant from K. lactis was cloned into Saccharomyces cerevisiae. Nucleotide sequence analysis of the determinant demonstrated that resistance was conferred by the K. lactis ribosomal protein L41. K. lactis was shown to contain only one copy of the gene that encodes this protein and the gene was located to chromosome III. In contrast, S. cerevisiae was found to contain multiple copies of the gene for the corresponding ribosomal protein L41 which mapped to two of the three chromosomes V, XIV and VIII. Since the cycloheximide-resistance gene of K. lactis causes essentially complete protection against inhibition by the drug, it is likely to be particularly useful as a selective marker in eukaryotic gene transfer studies.
Dehoux
P
P
Unité de Génie Microbiologique, Institut Pasteur, Paris, France.
Davies
J
J
Cannon
M
M
D000595:Amino Acid Sequence; D000818:Animals; D002874:Chromosome Mapping; D015825:Chromosomes, Fungal; D003001:Cloning, Molecular; D003513:Cycloheximide / Q000494:pharmacology*; D004271:DNA, Fungal / Q000235:genetics / Q000378:metabolism; D004352:Drug Resistance, Microbial / Q000235:genetics*; D005800:Genes, Fungal; D006801:Humans; D007716:Kluyveromyces / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010452:Peptide Biosynthesis; D010455:Peptides; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D015183:Restriction Mapping; D012269:Ribosomal Proteins / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D017386:Sequence Homology, Amino Acid
D004271:DNA, Fungal; D010455:Peptides; D012333:RNA, Messenger; D012269:Ribosomal Proteins; C012001:polyphenylalanine; D003513:Cycloheximide