Characterization of the renal handling of p-aminohippurate (PAH) in the beagle dog (Canis familiaris).
1. The renal clearance of p-aminohippurate (PAH) is the most commonly used method for estimation of renal blood flow in laboratory animals and in humans. 2. This study characterizes the renal handling of PAH in 11 beagle dogs and describes for this species critical parameters for the valid use of the renal PAH clearance technique to estimate renal blood flow. 3. PAH was given as a priming dose plus infusion rates of 0.01-6 mg/kg per min. 4. Steady-state plasma concentrations were achieved within 60 min. 5. Urinary excretion of PAH accounted for > or = 90% of the PAH infused. 6. Plasma PAH concentrations increased linearly with dose. 7. Renal secretion of PAH exceeded filtered PAH at plasma PAH concentrations less than approx. 6 mg%. 8. The TmPAH for renal PAH secretion was approx. 0.4 mg/kg per min. 9. At PAH infusion rates above TmPAH the clearance of PAH decreased and asymptotically approached the value of the GFR.
Mann
W A
WA
Department of Toxicology, SmithKline Beecham Pharmaceuticals, King of Prussia, PA 10406-0939.
Kinter
L B
LB
D000818:Animals; D004285:Dogs; D004573:Electrolytes / Q000652:urine; D005260:Female; D005919:Glomerular Filtration Rate; D006439:Hemodynamics / Q000187:drug effects; D007668:Kidney / Q000378:metabolism*; D008297:Male; D008657:Metabolic Clearance Rate; D012079:Renal Circulation; D010130:p-Aminohippuric Acid / Q000493:pharmacokinetics* / Q000494:pharmacology
D004573:Electrolytes; D010130:p-Aminohippuric Acid