Characterization of plasma membrane H(+)-ATPase from salt-tolerant yeast Candida versatilis.
Plasma membrane was isolated from the salt-tolerant yeast Candida versatilis and the ATPase in plasma membrane was characterized. The ATPase was a typical H(+)-ATPase with similar properties to the Saccharomyces cerevisiae and Zygosaccharomyces rouxii enzymes. It was reacted with antibody (IgG) raised against S. cerevisiae plasma membrane H(+)-ATPase. The ATPase activity was not changed by adding NaCl and KCl to the assay solutions, but was increased by NH4+, especially by ammonium sulfate. In vivo stimulation of ATPase activity was observed by the addition of NaCl into the culture medium, as observed in Z. rouxii. No in vivo activation of H(+)-ATPase by glucose metabolism was observed in C. versatilis cells and the activity was independent of the growth phase, like Z. rouxii and unlike S. cerevisiae cells.
Watanabe
Y
Y
Department of Biological Resources, Faculty of Agriculture, Ehime University, Japan.
Yamaguchi
M
M
Sakamoto
J
J
Tamai
Y
Y
D001386:Azides / Q000494:pharmacology; D002175:Candida / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development; D002414:Cations, Monovalent / Q000494:pharmacology; D002462:Cell Membrane / Q000187:drug effects / Q000201:enzymology*; D004024:Dicyclohexylcarbodiimide / Q000494:pharmacology; D005947:Glucose / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D008274:Magnesium / Q000494:pharmacology; D008982:Molybdenum / Q000494:pharmacology; D009566:Nitrates / Q000494:pharmacology; D009994:Osmolar Concentration; D017680:Potassium Compounds; D006180:Proton-Translocating ATPases / Q000187:drug effects / Q000378:metabolism*; D019810:Sodium Azide; D012965:Sodium Chloride / Q000494:pharmacology; D013997:Time Factors; D014638:Vanadates / Q000494:pharmacology
D001386:Azides; D002414:Cations, Monovalent; D009566:Nitrates; D017680:Potassium Compounds; D014638:Vanadates; D012965:Sodium Chloride; D004024:Dicyclohexylcarbodiimide; D008982:Molybdenum; D019810:Sodium Azide; D006180:Proton-Translocating ATPases; D008274:Magnesium; D005947:Glucose; C022175:ammonium molybdate; C023844:potassium nitrate