title: A robust, high-sensitivity algorithm for automated detection of proteins in two-dimensional electrophoresis gels.
abstract: The automated interpretation of two-dimensional gel electrophoresis images used in protein separation and analysis presents a formidable problem in the detection and characterization of ill-defined spatial objects. We describe in this paper a hierarchical algorithm that provides a robust, high-sensitivity solution to this problem, which can be easily adapted to a variety of experimental situations. The software implementation of this algorithm functions as part of a complete package designed for general protein gel analysis applications.
authors: Division of Biology, California Institute of Technology, Pasadena 91125.
authors: J E
authors: JE
authors: Solomon
authors: M G
authors: MG
authors: Harrington
mesh_terms: D000465:Algorithms; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D007091:Image Processing, Computer-Assisted; D011506:Proteins / Q000032:analysis*; D012984:Software
chemical_list: D011506:Proteins