title: [Nuclear proteins, specifically binding the regulatory region of rat tryptophan oxygenase].
abstract: Using electrophoretic mobility shift assay we have analysed the binding of rat liver nuclear proteins to the fragments from -466 to -292 and from -292 to -178 relative to the transcriptional start site of the rat tryptophan oxygenase gene. Studies of the competition with a synthetic consensus sequence for the NF 1 recognition site have shown that the liver nuclear proteins, responsible for the formation of specific complexes with these fragments belong to the family of the nuclear factor 1 (NF 1). We have also found that the trans-acting factors of the NF 1 family form several complexes with each of the two tested fragments of the tryptophan oxygenase gene.
authors: G I
authors: GI
authors: Chikhirzhina
authors: S A
authors: SA
authors: Fedorova
authors: I N
authors: IN
authors: Chespokov
authors: E V
authors: EV
authors: Romanovskaia
mesh_terms: D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D008099:Liver / Q000201:enzymology; D008297:Male; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000378:metabolism*; D051381:Rats; D012045:Regulatory Sequences, Nucleic Acid; D014158:Transcription, Genetic; D014366:Tryptophan Oxygenase / Q000235:genetics*
chemical_list: D009687:Nuclear Proteins; D014366:Tryptophan Oxygenase
vernacular_title: Iadernye belki, spetsificheski sviazyvaiushchiesia s reguliatornoĭ oblast'iu gena triptofanoksigenazy krysy.