Interleukin 3 promotes histamine synthesis in hematopoietic progenitors by increasing histidine decarboxylase mRNA expression.
Interleukin 3 (IL-3) is a potent stimulator of histamine production by cells from murine hematopoietic organs. We demonstrate herein that this phenomenon results from increased histidine decarboxylase (HDC: EC 4.1.1.22) activity in progenitor-enriched bone marrow cells (around 5% of the total bone marrow) isolated from the low density layers of a discontinuous Ficoll gradient. HDC levels are markedly enhanced after a 24 h incubation with IL-3 while a 4 h exposure results only in a slight activation. It results from increased expression of the mRNA coding for HDC, as assessed by Northern blot analysis after a 24 h incubation with IL-3. At the same time point and after a 4 h stimulation, we have evaluated the percentage of cells in this population which express HDC mRNA in response to IL-3, using in situ hybridization with the antisense riboprobe. We have thus established that enhanced HDC mRNA expression occurs in a small immature subset representing from 5 to 8% of the progenitor-enriched bone marrow cells.
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CNRS URA 1461, Hôpital Necker, Paris, France.
Machavoine
F
F
Lebel
B
B
Ichikawa
A
A
Gastinel
L N
LN
Schneider
E
E
D000818:Animals; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D006412:Hematopoietic Stem Cells / Q000187:drug effects* / Q000378:metabolism; D006632:Histamine / Q000096:biosynthesis*; D006640:Histidine Decarboxylase / Q000096:biosynthesis* / Q000235:genetics; D017403:In Situ Hybridization; D007377:Interleukin-3 / Q000494:pharmacology*; D051379:Mice; D008810:Mice, Inbred C57BL; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics
D007377:Interleukin-3; D012333:RNA, Messenger; D006632:Histamine; D006640:Histidine Decarboxylase