Polypeptide synthesis induced in Nicotiana clevelandii protoplasts by infection with raspberry ringspot nepovirus.
Infection of Nicotiana clevelandii protoplasts by raspberry ringspot nepovirus resulted in the accumulation of about 24 polypeptides that differed in M(r) and pI from polypeptides accumulating in mock-inoculated protoplasts. Similar polypeptides accumulated in protoplasts infected with the S and E strains of RRV but different infection-specific polypeptides were detected in protoplasts infected with tobacco ringspot nepovirus. The M(r) of RRV-specific polypeptides ranged from 210,000 to 18,000 and most are presumed to be derived from others by proteolytic cleavage. No evidence was found for marked changes in polypeptide abundance with time after inoculation or for any virus-specific polypeptide becoming disproportionately abundant in the medium during culture.
Acosta
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Scottish Crop Research Institute, Invergowrie, Dundee, Scotland.
Mayo
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D002213:Capsid / Q000032:analysis; D003470:Culture Media; D015180:Electrophoresis, Gel, Two-Dimensional; D005638:Fruit / Q000382:microbiology*; D008970:Molecular Weight; D010942:Plant Viruses / Q000378:metabolism*; D011523:Protoplasts / Q000378:metabolism; D012367:RNA, Viral / Q000235:genetics; D014764:Viral Proteins / Q000032:analysis / Q000096:biosynthesis*
D003470:Culture Media; D012367:RNA, Viral; D014764:Viral Proteins