Amino acid sequence of alkaliphilic serine protease from silkworm, Bombyx mori, larval digestive juice.
Alkaliphilic protease, P-IIc, from silkworm, Bombyx mori, larval midgut digestive juice consists of 232 amino acids. It has a catalytic triad, Asp-His-Ser, invariably found in a serine protease. A shift of optimal pH value towards the alkaline side diminished at mu = 1.0. This suggests the existence of an electrostatic interaction that affects the proteolytic activity. The higher Arg content may be responsible for this phenomenon. Two cysteine residues probably exist unpaired in a novel position among serine proteases.
Sasaki
T
T
Department of Food Science and Technology, School of Agriculture, Nagoya University, Aichi, Japan.
Hishida
T
T
Ichikawa
K
K
Asari
S
S
D000595:Amino Acid Sequence; D000818:Animals; D012831:Bombyx; D005750:Gastric Juice / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D007814:Larva; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D012697:Serine Endopeptidases / Q000737:chemistry*
D012697:Serine Endopeptidases