Strand-specific FISH reveals orientation of chromosome 18 alphoid DNA.
A method is described for making in situ hybridization strand specific. Through the use of a synthetic DNA probe of a repetitive sequence in the centromeric region of chromosome 18, it is shown that the repeats exist in a head-to-tail tandem array. The method should be useful for studies of the molecular organization and mapping of chromosomes.
Goodwin
E
E
Life Sciences Division, Los Alamos National Laboratory, NM 87545.
Meyne
J
J
D001483:Base Sequence; D002460:Cell Line; D002887:Chromosomes, Human, Pair 18; D004247:DNA / Q000235:genetics; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008969:Molecular Sequence Data; D012091:Repetitive Sequences, Nucleic Acid
D004247:DNA