B16-G4F mouse melanoma cells: an MSH receptor-deficient cell clone.
The two mouse melanoma cell lines B16-F1 and B16-G4F retain their melanogenic capacity when cultured in vitro. Melanotropic peptides such as alpha-melanocyte-stimulating hormone (alpha-MSH) induce formation and release of melanin pigment in B16-F1 cells. In contrast, B16-G4F cells do not respond to alpha-MSH. Using receptor-binding analysis and photoaffinity crosslinking we demonstrate that the lack of response of B16-G4F cells to alpha-MSH is due to the absence of functional MSH receptors from the cell surface. Northern blot analysis of receptor mRNA revealed that MSH receptor mRNA is not expressed in B16-G4F cells. These cells represent a new tool for the study of signal pathways related to the control of melanogenesis in melanoma cells.
Solca
F F
FF
Department of Research (ZLF), University Hospital, Basel, Switzerland.
Chluba-de Tapia
J
J
Iwata
K
K
Eberle
A N
AN
D000818:Animals; D001483:Base Sequence; D002999:Clone Cells; D009074:Melanocyte-Stimulating Hormones / Q000378:metabolism*; D008546:Melanoma, Experimental / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D011979:Receptors, Pituitary Hormone / Q000378:metabolism*; D014407:Tumor Cells, Cultured / Q000378:metabolism*
D011979:Receptors, Pituitary Hormone; C029446:MSH receptor; D009074:Melanocyte-Stimulating Hormones