Histochemical evidence for induction of arachidonate 15-lipoxygenase in airway disease.
We have previously described the distribution of the arachidonate 15-lipoxygenase in lung tissue obtained from healthy human subjects. In the present study, we have utilized the same immunohistochemical methodology to examine the expression of 15-lipoxygenase in bronchial biopsy tissue from subjects with airway disease. Immunohistochemistry of bronchial tissue using two antibodies against distinct epitopes of the 15-lipoxygenase and indirect biotin-avidin-peroxidase detection demonstrated that, in contrast to airway tissue from normal subjects (n = 10) in which 15-lipoxygenase antigen was confined to the uppermost airways (nose and trachea) and was almost undetectable in bronchi, the bronchial tissue obtained from subjects with asthma (n = 7) or chronic bronchitis (n = 7) exhibited markedly positive immunostaining of mucosal epithelial cells with both anti-15-lipoxygenase antibodies. Specificity of 15-lipoxygenase immunostaining was verified by antigen competition experiments and by the lack of immunostaining with preimmune serum or control anti-5-lipoxygenase antibodies. The increased levels of 15-lipoxygenase antigen in the bronchial epithelial cells of asthmatic and bronchitic subjects compared with the same cell population in normal subjects coupled with the previous findings of increased 15-lipoxygenase activity in asthmatic airways suggest that epithelial 15-lipoxygenase is induced by airway inflammatory disease.
Shannon
V R
VR
Department of Medicine, Washington University School of Medicine, St. Louis, Missouri.
Chanez
P
P
Bousquet
J
J
Holtzman
M J
MJ
D000293:Adolescent; D000328:Adult; D001093:Arachidonate 15-Lipoxygenase / Q000032:analysis / Q000096:biosynthesis*; D001249:Asthma / Q000201:enzymology*; D001980:Bronchi / Q000201:enzymology*; D001991:Bronchitis / Q000201:enzymology*; D002908:Chronic Disease; D004790:Enzyme Induction; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged
D001093:Arachidonate 15-Lipoxygenase