title: A conserved region of the MSP-1 surface protein of Plasmodium falciparum contains a recognition sequence for erythrocyte spectrin.
abstract: The major surface protein MSP-1 of Plasmodium falciparum blood-stage malaria parasites contains notably conserved sequence blocks with unknown function. The recombinant protein 190L, which represents such a block, exhibits a high affinity for red blood cell membranes. We demonstrate that both 190L and native MSP-1 protein bind to the inner red blood cell membrane skeleton protein spectrin. By using overlapping peptides covering the 190L molecule, we show that the spectrin contact site of 190L is included in a linear sequence of 30 amino acid residues. Association of 190L with naturally occurring spectrin deficient red blood cells is drastically reduced. In the same cells parasite invasion is normal, but the intracellular parasite development arrests late in the trophozoite stage. A similar situation arises when synthetic peptides covering the spectrin recognition sequence of 190L are added to P.falciparum cultures. These data and the cellular localization of MSP-1 suggest the possibility that MSP-1 associates with spectrin under natural conditions.
authors: Department of Microbiology, School of Health, Universidad del Valle, Cali, Colombia.
authors: S
authors: S
authors: Herrera
authors: W
authors: W
authors: Rudin
authors: M
authors: M
authors: Herrera
authors: P
authors: P
authors: Clavijo
authors: L
authors: L
authors: Mancilla
authors: C
authors: C
authors: de Plata
authors: H
authors: H
authors: Matile
authors: U
authors: U
authors: Certa
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000953:Antigens, Protozoan / Q000378:metabolism*; D001665:Binding Sites; D004910:Erythrocyte Membrane / Q000378:metabolism; D004912:Erythrocytes / Q000469:parasitology*; D006801:Humans; D066298:In Vitro Techniques; D020066:Merozoite Surface Protein 1; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000378:metabolism; D010963:Plasmodium falciparum / Q000378:metabolism*; D011498:Protein Precursors / Q000378:metabolism*; D015800:Protozoan Proteins / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D013049:Spectrin / Q000378:metabolism*
chemical_list: D000953:Antigens, Protozoan; D020066:Merozoite Surface Protein 1; D010446:Peptide Fragments; D011498:Protein Precursors; D015800:Protozoan Proteins; D011994:Recombinant Proteins; D013049:Spectrin