Distribution of [241Am] in hepato-pancreatic nuclear proteins of the lobster (Homarus gammarus).
Recent studies on the bio-accumulation of transuranic elements in marine invertebrates have demonstrated an intracellular concentration of radionuclides in the digestive gland. The present investigation aims to establish the distribution of [241Am] within the different structures of the cell nucleus. Lobsters were experimentally contaminated through feeding and then killed. The nuclei of hepato-pancreatic cells were isolated, purified and dissociated. The different protein categories were separated in media of increasing ionic strength. The distribution of americium in this material yielded results showing a preferential fixation onto the structural proteins of the nuclear matrix. The radiological consequences of such an affinity are discussed in terms of the life-times of the ligands concerned.
Paquet
F
F
Département de Protection de l'Environnement et des Installations, IPSN/CEA, Beaumont Hague, France.
Durand
J P
JP
Goudard
F
F
Germain
P
P
Milcent
M C
MC
Pieri
J
J
D000576:Americium / Q000032:analysis* / Q000378:metabolism; D000818:Animals; D002458:Cell Fractionation; D004247:DNA / Q000737:chemistry; D005260:Female; D008099:Liver / Q000737:chemistry / Q000166:cytology; D008297:Male; D008121:Nephropidae / Q000737:chemistry* / Q000378:metabolism; D009687:Nuclear Proteins / Q000737:chemistry*; D010179:Pancreas / Q000737:chemistry / Q000166:cytology
D009687:Nuclear Proteins; D004247:DNA; D000576:Americium