Characterization of the 5' flanking region of the gene encoding rat liver glycogen phosphorylase.
A genomic region encompassing 800 bp of the promoter-regulatory region and exon 1 of the gene (LGP) encoding rat liver glycogen phosphorylase has been isolated and characterized. Transcripts of the LGP gene initiate predominantly within an 8-bp region 48-bp upstream from the start codon. Additional transcripts were detected that initiate as far as 95 bp upstream from the start codon. To identify cis-acting sequences involved in regulating transcription, HepG2 cells were transfected with vectors containing serial deletions of the promoter-regulatory region of LGP ligated to the cat reporter gene. Two upstream regions were found to enhance transcription. One of these regions contains an alternating purine-pyrimidine sequence. LGP, which lacks a consensus TATA sequence, is like TATA-less and CAAT-less housekeeping genes in that it contains G + C-rich domains upstream from multiple transcription start points. Nuclear proteins from adult rat tissues bound in a tissue-specific fashion to one of these G + C-rich regions.
Herrick
K R
KR
Department of Neurology, University of California, Davis 95616-8647.
Gorin
F A
FA
Park
E A
EA
Tait
R C
RC
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics / Q000378:metabolism; D003001:Cloning, Molecular; D004247:DNA; D004268:DNA-Binding Proteins / Q000378:metabolism; D005091:Exons; D006801:Humans; D007438:Introns; D008099:Liver / Q000201:enzymology*; D008969:Molecular Sequence Data; D009928:Organ Specificity / Q000235:genetics; D006005:Phosphorylases / Q000235:genetics* / Q000378:metabolism; D051381:Rats; D015183:Restriction Mapping; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
D004268:DNA-Binding Proteins; D004247:DNA; D015500:Chloramphenicol O-Acetyltransferase; D006005:Phosphorylases