Imaging frozen, hydrated acrosomal bundle from Limulus sperm at 7 A resolution with a 400 kV electron cryomicroscope.
Actin and scruin are present in a highly ordered array in the acrosomal bundle from Limulus sperm. Frozen, hydrated acrosomal bundle can be imaged in a 400 kV electron cryomicroscope using the spot-scan technique. Quantitative analysis of the h0l projection images shows diffraction data beyond 7 A.
Schmid
M F
MF
Verna and Marrs McLean Department of Biochemistry, Baylor College of Medicine, Houston, TX 77030.
Jakana
J
J
Matsudaira
P
P
Chiu
W
W
D000177:Acrosome / Q000648:ultrastructure*; D000818:Animals; D005615:Freezing; D006737:Horseshoe Crabs; D008297:Male; D008854:Microscopy, Electron / Q000379:methods; D013094:Spermatozoa / Q000648:ultrastructure*