The first specific antibody against cytotoxic polyacetylenic alcohol, panaxynol.
Antitumor polyacetylenic alcohol, panaxynol, was isolated and purified from a powder of the root of Panax ginseng C.A. Meyer. Panaxynol inhibited the growth of various kinds of cultured tumor cell lines in a dose-dependent manner. In this paper we demonstrated the first specific antibody production against panaxynol. Anti-panaxynol antibody was elicited in rabbits by immunization with panaxynol hemisuccinate-bovine serum albumin conjugate (panaxynol hemisuccinate-BSA conjugate). An enzyme immunoassay (EIA) for the determination of panaxynol was established using a double-antibody technique. The EIA was highly specific against panaxynol although the antibody showed a minimal cross-reactivity with other types of polyacetylenic alcohol, i.e. panaxydol (12.0%) and panaxytriol (0.77%). Panaxynol at a concentration as low as 6.4 ng/ml can be detected. Using this assay we reconfirmed the rapid consumption of panaxynol by target tumor cells in an in vitro-culture system. The anti-panaxynol antibody may be a valuable tool for studies of the biological properties of polyacetylenic compounds.
Saita
T
T
Hospital Pharmacy, Department of Surgery, Saga, Japan.
Katano
M
M
Matsunaga
H
H
Yamamoto
H
H
Fujito
H
H
Mori
M
M
D000480:Alkynes; D000818:Animals; D000906:Antibodies / Q000276:immunology*; D000917:Antibody Formation; D000918:Antibody Specificity; D000972:Antineoplastic Agents, Phytogenic / Q000276:immunology* / Q000493:pharmacokinetics / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects*; D003429:Cross Reactions; D053280:Diynes; D004365:Drugs, Chinese Herbal / Q000493:pharmacokinetics / Q000494:pharmacology; D053281:Enediynes; D005233:Fatty Alcohols / Q000276:immunology* / Q000493:pharmacokinetics / Q000494:pharmacology; D006801:Humans; D007114:Immunization; D007124:Immunoenzyme Techniques; D011817:Rabbits; D014407:Tumor Cells, Cultured
D000480:Alkynes; D000906:Antibodies; D000972:Antineoplastic Agents, Phytogenic; D053280:Diynes; D004365:Drugs, Chinese Herbal; D053281:Enediynes; D005233:Fatty Alcohols; C018541:falcarinol; C057682:panaxytriol