title: Tumor cell growth arrest caused by subchromosomal transferable DNA fragments from chromosome 11.
abstract: A fundamental problem in the identification and isolation of tumor suppressor and other growth-inhibiting genes is the loss of power of genetic complementation at the subchromosomal level. A direct genetic strategy was developed to isolate subchromosomal transferable fragments (STFs) from any chromosome, each containing a selectable marker within the human DNA, that could be transferred to any mammalian cell. As a test of the method, several overlapping STFs from 11p15 were shown to cause in vitro growth arrest of rhabdomyosarcoma cells. This activity mapped between the beta-globin and insulin genes.
authors: Howard Hughes Medical Institute, Department of Internal Medicine, University of Michigan Medical School, Ann Arbor 48109.
authors: M
authors: M
authors: Koi
authors: L A
authors: LA
authors: Johnson
authors: L M
authors: LM
authors: Kalikin
authors: P F
authors: PF
authors: Little
authors: Y
authors: Y
authors: Nakamura
authors: A P
authors: AP
authors: Feinberg
mesh_terms: D000818:Animals; D001483:Base Sequence; D016466:CHO Cells; D002455:Cell Division; D002460:Cell Line; D002880:Chromosomes, Human, Pair 11; D006224:Cricetinae; D004247:DNA / Q000235:genetics*; D016147:Genes, Tumor Suppressor; D005819:Genetic Markers; D005821:Genetic Techniques; D005914:Globins / Q000235:genetics; D006801:Humans; D007328:Insulin / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D012208:Rhabdomyosarcoma / Q000473:pathology*; D014407:Tumor Cells, Cultured
chemical_list: D005819:Genetic Markers; D007328:Insulin; D005914:Globins; D004247:DNA