Effects of season and estradiol on concentrations of histamine within discrete brain regions of ovariectomized ewes.
Histamine has been implicated as a neuromodulator of secretion of gonadotropins in several species. Concentrations of histamine were analyzed within discrete brain regions and endocrine tissues to help determine whether this amine has the potential to exert a similar function in ewes expected to have dramatically different serum concentrations of LH. Following collection of blood samples at 12-min intervals for 4-hr, ovariectomized (OVX) and OVX-estradiol treated (OVX-E) ewes were slaughtered during the breeding and anestrous seasons (five animals/group). Concentrations of LH were depressed by treatment with estradiol (E; P < .01), but to a greater extent (P < .05) during the anestrous season compared to the breeding season. Concentrations of histamine in tissues (ng/mg) differed (P < .01) between the breeding and anestrous seasons, in the medial thalamus (39.2 +/- 14.1 vs 109.9 +/- 13.0), posterior pituitary gland (247.6 +/- 50.7 vs 23.0 +/- 9.1) and midbrain tegmentum (10.4 +/- 5.6 vs 50.7 +/- 3.9). Estradiol containing implants decreased (P < .05) concentrations of histamine in the midbrain tegmentum (20.3 +/- 7.1 vs 37.7 +/- 7.8) and posterior pituitary gland (87.3 +/- 24.0 vs 258.2 +2- 67.5) compared to non-estradiol treated controls. Histamine concentrations in the pineal and anterior pituitary glands and brain regions; stalk-median eminence, medial basal hypothalamus, preoptic area, cerebellum, parietal neocortex, were not (P > .05) affected solely by either season or E. An interaction between effects of season and estradiol on concentrations of histamine occurred (P < .05) in the posterior pituitary gland and the preoptic area.(ABSTRACT TRUNCATED AT 250 WORDS)
Alexander
B M
BM
Department of Animal Science, University of Wyoming, Laramie 82071.
Van Kirk
E A
EA
Rose
J D
JD
Murdoch
W J
WJ
Moss
G E
GE
D000818:Animals; D001923:Brain Chemistry / Q000187:drug effects; D004958:Estradiol / Q000494:pharmacology*; D004971:Estrus / Q000378:metabolism*; D005260:Female; D006632:Histamine / Q000032:analysis* / Q000502:physiology; D007031:Hypothalamus / Q000737:chemistry; D007986:Luteinizing Hormone / Q000097:blood; D008473:Median Eminence / Q000737:chemistry; D010052:Ovariectomy / Q000662:veterinary; D010870:Pineal Gland / Q000737:chemistry; D010903:Pituitary Gland, Anterior / Q000737:chemistry; D010904:Pituitary Gland, Posterior / Q000737:chemistry; D011301:Preoptic Area / Q000737:chemistry; D012621:Seasons; D012756:Sheep / Q000378:metabolism*; D013788:Thalamus / Q000737:chemistry
D004958:Estradiol; D006632:Histamine; D007986:Luteinizing Hormone