Genetic organization and sequence of the region encoding integrative functions from Lactobacillus gasseri temperate bacteriophage phi adh.
A 2.0-kb fragment from the Lactobacillus gasseri temperate bacteriophage phi adh contained the essential genetic determinants for site-specific integration. The nucleotide sequence of this fragment was determined. An open reading frame (intG), which adjoined the phage attachment site (attP), encoded a deduced protein related to the integrase family. The organization of this region was comparable to other phage site-specific recombination systems.
Fremaux
C
C
Department of Food Science, North Carolina State University, Raleigh 27695-7624.
De Antoni
G L
GL
Raya
R R
RR
Klaenhammer
T R
TR
D000595:Amino Acid Sequence; D001435:Bacteriophages / Q000201:enzymology / Q000235:genetics*; D001483:Base Sequence; D004254:DNA Nucleotidyltransferases / Q000235:genetics / Q000378:metabolism; D004279:DNA, Viral; D019426:Integrases; D007778:Lactobacillus / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010957:Plasmids; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D016662:Virus Integration / Q000235:genetics*
D004279:DNA, Viral; D004254:DNA Nucleotidyltransferases; D019426:Integrases