Expression of members of the heat-shock protein 70 family in the exoerythrocytic stages of Plasmodium berghei and Plasmodium falciparum.
Exoerythrocytic stages of Plasmodium berghei cultured in HepG2-A16 hepatoma cells and those of P. falciparum in human hepatocytes transplanted under the kidney capsule of CB-17/ICr scid/scid mice were used to evaluate expression of heat-shock-related stress proteins. Although undetectable in the sporozoites, the expression of proteins similar in sequence of a heat-shock protein of 70 kDa and a glucose-regulated protein of 78 kDa was markedly induced in the hepatic stages of malaria parasites. Expression of these proteins in the exoerythrocytic stages of the malaria parasite warrants a systematic evaluation of their potential role in eliciting cellular immune responses directed against infected hepatocytes.
Kumar
N
N
Department of Immunology and Infectious Diseases, School of Hygiene and Public Health, Johns Hopkins University, Baltimore, MD 21205.
Nagasawa
H
H
Sacci
J B
JB
Sina
B J
BJ
Aikawa
M
M
Atkinson
C
C
Uparanukraw
P
P
Kubiak
L B
LB
Azad
A F
AF
Hollingdale
M R
MR
D000818:Animals; D000852:Anopheles / Q000469:parasitology; D004912:Erythrocytes; D005455:Fluorescent Antibody Technique; D006360:Heat-Shock Proteins / Q000032:analysis / Q000096:biosynthesis* / Q000302:isolation & purification; D006801:Humans; D015151:Immunoblotting; D008099:Liver / Q000469:parasitology*; D016031:Liver Transplantation; D008288:Malaria / Q000503:physiopathology*; D051379:Mice; D016513:Mice, SCID; D008970:Molecular Weight; D010962:Plasmodium berghei / Q000472:pathogenicity / Q000502:physiology*; D010963:Plasmodium falciparum / Q000472:pathogenicity / Q000502:physiology*; D012469:Salivary Glands / Q000469:parasitology; D014183:Transplantation, Heterologous; D016042:Transplantation, Heterotopic; D014407:Tumor Cells, Cultured
D006360:Heat-Shock Proteins