Confirmation of the airborne occurrence of micron-size airborne pollen antigen carrying particles by immunoblotting.
We confirmed the existence of micron-size airborne particles carrying major pollen antigen of Cryptomeria japonica (Cry j l) and Lolium perenne (Lol p l) by means of an immunoblotting technique and examination by light microscopy. The size of the spots on a nitrocellulose membrane, marking the presence of antigen on the sampler's tape, varied considerably under light microscopic examination. Spots smaller than pollen grains and micron-size spots were observed together with large spots which seem to have been derived from intact pollen grains. The number of micron-size spots counted under a light microscope did not correspond with the number of spots from pollen grain observable with the naked eyes. This suggests other sources of airborne Cry j l particles than intact pollen grains alone.
Takahashi
Y
Y
Yamagata Prefectural Institute of Public Health, Japan.
Sakaguchi
M
M
Inouye
S
S
Nagoya
T
T
Watanabe
M
M
Yasueda
H
H
D000388:Air; D000941:Antigens / Q000302:isolation & purification*; D015151:Immunoblotting; D008129:Lolium; D010316:Particle Size; D011058:Pollen
D000941:Antigens