Laser-assisted microvascular anastomosis of human adult and placental arteries with expanded polytetrafluoroethylene microconduit.
Laser-assisted microvascular anastomoses can be performed more quickly than sutured anastomoses, yet manifest similar patency rates and tensile strength. This study was undertaken to determine if in vitro laser-assisted microvascular anastomoses could be created between human adult arteries (anterior tibial arteries), human placental arteries, and expanded polytetrafluoroethylene microconduits. A CO2 laser was applied in single or continuous bursts with a matrix of variables encompassing power P = 80 to 160 mW, spot size SS = 150 to 500 microns, and exposure time EXP = 1.0-second continuous exposure (n = 2 each composite setting). The endpoints measured to assess the ability to laser-weld vessels were morphologic appearance by scanning electron microscopy and bursting strength. Scanning electron microscopy revealed apparent fusion of human placental arteries and human adult arteries to expanded polytetrafluoroethylene microconduits at settings of P = 130 mW, SS = 300 microns, and EXP = 1.0 second, though bursting pressure at all settings was less than 10 mmHg. Laser-assisted microvascular anastomoses of human placental artery to human placental artery and human adult artery to human adult artery were successful at this setting, though bursting pressures of anastomoses incorporating placental vessels were significantly weaker than those created with adult tissue. The relative weakness of laser-assisted microvascular anastomoses incorporating placental arteries might be explained by qualitative or quantitative differences in vessel wall collagen, as seen in fetal tissue, and deserves further characterization.
Bentz
M L
ML
Division of Plastic and Reconstructive Surgery, University of Pittsburgh School of Medicine, Pa.
Parva
B
B
Dickson
C S
CS
Futrell
J W
JW
Johnson
P C
PC
D000328:Adult; D000714:Anastomosis, Surgical; D001158:Arteries / Q000601:surgery* / Q000648:ultrastructure; D001696:Biomechanical Phenomena; D001807:Blood Vessel Prosthesis; D006801:Humans; D066298:In Vitro Techniques; D053685:Laser Therapy; D008833:Microcirculation / Q000601:surgery; D008855:Microscopy, Electron, Scanning; D008866:Microsurgery / Q000379:methods*; D010920:Placenta / Q000098:blood supply; D011138:Polytetrafluoroethylene
D011138:Polytetrafluoroethylene