Thickness determination of biological thin sections by multiple-least-squares fitting of the carbon K-edge in the electron energy-loss spectrum.
We show that the local thickness of biological thin sections can be measured by fitting the electron energy-loss spectrum of the plurally scattered carbon K-edge, and that such measurements are consistent with the results obtained by a conventional method based on the relationship t/lambda alpha ln(It/I0). The new method allows the simultaneous measurement and/or mapping of both local thickness and core-level excitations (e.g. Ca L-edge) in this energy range (280-400 eV) without requiring the measurement of the zero-loss and the valence/plasmon excitations.
Zhao
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Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville 22908.
Wang
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Ho
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Shao
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Somlyo
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Somlyo
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AP
D000818:Animals; D002244:Carbon; D004577:Electron Probe Microanalysis / Q000379:methods*; D016018:Least-Squares Analysis; D051381:Rats
D002244:Carbon