title: Zinc protection against cadmium-induced destruction of the monolayer of cultured vascular endothelial cells.
abstract: The effect of zinc on the destruction of vascular endothelial cell monolayer caused by cadmium was investigated using bovine aortic endothelial cells in a culture system. It was histologically revealed that cadmium-induced de-endothelialized area formation was markedly prevented by zinc. Zinc prevented cadmium-induced detachment of [3H]thymidine-labeled endothelial cells from the monolayer. Although cadmium increased the activity of lactate dehydrogenase in the medium, a marker of nonspecific cell damage, zinc markedly suppressed it. On the other hand, zinc significantly prevented cadmium-induced decrease in the incorporation of [3H]thymidine into the acid-insoluble fraction of the growing cells. From these results, it was suggested that impairment of the endothelial cell monolayer caused by cadmium is protected by zinc; this protection was postulated to be due to both a reduction of the cell detachment caused by cadmium-induced nonspecific cell damage and a decrease in cadmium inhibition of the cell proliferation which would help the repair of de-endothelialized areas.
authors: Department of Environmental Science, Faculty of Pharmaceutical Sciences, Hokuriku University, Kanazawa, Japan.
authors: T
authors: T
authors: Kaji
authors: A
authors: A
authors: Mishima
authors: C
authors: C
authors: Yamamoto
authors: M
authors: M
authors: Sakamoto
authors: H
authors: H
authors: Kozuka
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001011:Aorta; D002104:Cadmium / Q000633:toxicity*; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D015032:Zinc / Q000494:pharmacology*
chemical_list: D002104:Cadmium; D007770:L-Lactate Dehydrogenase; D015032:Zinc