title: Application of chemical cytochrome P-450 model systems to studies on drug metabolism. VI. N,N-coupling reaction of N-methylaniline catalyzed by polypeptide-bound porphyrinatoiron(III) and cytochrome P-450.
abstract: Polypeptide-bound porphyrinatoiron(III) effectively catalyzed the N,N-coupling reaction of N-methylaniline in the presence of NaBH4/O2/tetramethyl-ammonium hydroxide or cumenehydroperoxide, whereas the corresponding non-bound monomer porphyrinatoiron(III) had little catalytic activity. Rat liver microsomes also catalyzed the N,N-coupling which was inhibited by cytochrome P-450 specific inhibitors, SKF-525A and metyrapone. The polypeptide-bound porphinatoiron(III) appears to be an excellent cytochrome P-450 model for drug metabolism studies.
authors: Faculty of Pharmaceutical Sciences, University of Tokyo, Japan.
authors: T
authors: T
authors: Doi
authors: T
authors: T
authors: Mori
authors: T
authors: T
authors: Mashino
authors: M
authors: M
authors: Hirobe
mesh_terms: D000814:Aniline Compounds / Q000737:chemistry / Q000378:metabolism*; D000818:Animals; D002384:Catalysis; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D005290:Ferric Compounds / Q000378:metabolism*; D008665:Metalloporphyrins / Q000378:metabolism*; D008862:Microsomes, Liver / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism; D010455:Peptides / Q000378:metabolism*; D004364:Pharmaceutical Preparations / Q000378:metabolism*; D051381:Rats
chemical_list: D000814:Aniline Compounds; D005290:Ferric Compounds; D008665:Metalloporphyrins; D010455:Peptides; D004364:Pharmaceutical Preparations; D009249:NADP; D003577:Cytochrome P-450 Enzyme System; D010100:Oxygen; C021313:methylaniline