Identification of a putative cellular receptor for HTLV-I by a monoclonal antibody, Mab 34-23.
The ability of HTLV-I to infect cells is presumed to be dependent, in some part, on the attachment of the virus to a target cell via a specific cell surface receptor which is, as yet, unknown. Here we present evidence that a monoclonal antibody, Mab 34-23, inhibits the binding of HTLV-I to IL-2 and phytohemagglutinin-activated peripheral blood mononuclear cells and also inhibits virus entry into these cells. Analysis of a variety of target cells, including a human:mouse somatic hybrid which contains only human chromosome 17q, indicates that the binding of Mab 34-23 correlates with HTLV-I adsorption and entry. SDS-PAGE and Western blot analysis show that Mab 34-23 binds to four major proteins of MW 31, 45, 55, and 70 kDa and this binding can be inhibited by HTLV-I and not HIV proteins. HTLV-I virions bind to proteins of similar molecular weight and virus-binding to these proteins can be inhibited by preincubation with Mab 34-23. These data suggest that Mab 34-23 may identify a specific cell surface receptor(s) for HTLV-I.
Gavalchin
J
J
Department of Medicine, SUNY Health Science Center, New York 13210.
Fan
N
N
Lane
M J
MJ
Papsidero
L
L
Poiesz
B J
BJ
D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D015153:Blotting, Western; D002460:Cell Line; D015870:Gene Expression; D015481:HTLV-I Antibodies / Q000276:immunology*; D015486:HTLV-I Antigens / Q000096:biosynthesis / Q000276:immunology / Q000302:isolation & purification*; D015368:Human T-lymphotropic virus 1 / Q000378:metabolism*; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000166:cytology; D010835:Phytohemagglutinins / Q000494:pharmacology; D011991:Receptors, Virus / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000276:immunology / Q000302:isolation & purification*; D014764:Viral Proteins / Q000096:biosynthesis / Q000276:immunology / Q000302:isolation & purification*
D000911:Antibodies, Monoclonal; D015481:HTLV-I Antibodies; D015486:HTLV-I Antigens; C080823:HTLV-I receptor; D007376:Interleukin-2; D010835:Phytohemagglutinins; D011991:Receptors, Virus; D014764:Viral Proteins