[Use of hydrazine for rapid deblocking of synthetic oligonucleotides].
A rapid method for the removal of protecting groups from synthetic oligodeoxyribonucleotides, obtained by the phosphoroamidite and H-phosphonate methods, including the use of hydrazine solutions has been developed. The combination of this procedure with the application of isopropoxyacetyl N-protecting group and oxalyl ester linkage for the first nucleoside attachment to the polymer support allows to reduce to several minutes the time needed for the full oligonucleotide deprotection.
Polushin
N N
NN
Pashkova
I N
IN
Chakhmakhcheva
O G
OG
Efimov
V A
VA
D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D006834:Hydrazines / Q000737:chemistry*; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*
D006834:Hydrazines; D009838:Oligodeoxyribonucleotides; C029424:hydrazine
Primenenie gidrazina dlia bystrogo deblokirovaniia sintetitcheskikh oligonukleotidov.