Distribution of lectin binding sites in the oviducts of cycling and hormone-treated pigs.
Gamete transport, fertilization, and early embryonic development take place in different regions of the oviduct and under different hormonal conditions. The objective of this study was to use lectins to detect variation in the distribution of glycosylated molecules on the surface of the epithelia that influence these events. Oviducts were collected from gilts on day 1 (estrus) and day 16 (diestrus) of the estrous cycle, and from gilts that were bilaterally ovariectomized on day 4 of the estrous cycle, and were subsequently treated with estradiol valerate (100 micrograms/day), progesterone (200 mg/day), or corn oil vehicle for 11 days. Six biotinylated lectins, Triticum vulgaris (WGA), Arachis hypogaea (PNA), Ulex europeus (UEA-I), Dolichos biflorus (DBA), Ricinus communis (RCA-I), and Canavalia ensiformis (Con A), were used to probe tissue from the isthmus and ampulla using peroxidase-conjugated avidin as the marker. In cyclic gilts, WGA and DBA stained more strongly in the isthmus than the ampulla on both cycle days. Staining with PNA was patchy, but greater on the apical surface of isthmic epithelium on day 16 than day 1. With UEA-I, staining was more intense in the ampulla on day 16 than day 1. Staining by PNA and UEA-I was suppressed in both the ampulla and isthmus with estradiol treatment. DBA staining was suppressed in the isthmus with both estradiol and progesterone treatments. Cilia were labelled by all lectins except WGA and PNA. Thus glycosylation patterns vary in the porcine oviduct with region and hormonal state, reflecting the variety of events that may be influenced.
Raychoudhury
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SS
Department of Physiological Sciences, College of Veterinary Medicine, University of Florida, Gainesville 32610.
Suarez
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SS
Buhi
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WC
D000818:Animals; D001665:Binding Sites; D004958:Estradiol / Q000502:physiology; D004971:Estrus; D005187:Fallopian Tubes / Q000378:metabolism*; D005260:Female; D006001:Glycoconjugates / Q000378:metabolism; D037102:Lectins / Q000378:metabolism*; D010052:Ovariectomy; D011374:Progesterone / Q000502:physiology; D013552:Swine
D006001:Glycoconjugates; D037102:Lectins; D011374:Progesterone; D004958:Estradiol