Approaches to the identification and classification of chemical allergens in mice.
During the last 10 years understanding of the immunobiological mechanisms whereby chemicals induce allergy has increased significantly. In parallel there have emerged opportunities to develop alternative methods for the prospective identification and classification of chemical contact and respiratory allergens. Attention has focused largely on the mouse, and several novel approaches to the identification of contact allergens have been described, among them the local lymph node assay, the mouse ear swelling test and, most recently, the noninvasive mouse ear swelling assay (MESA). Progress has been made also in defining methods for measuring respiratory sensitization potential in mice. Recent evidence indicates that respiratory and contact allergens provoke qualitatively different immune responses characteristic of selective T helper (Th) cell activation. Evaluation of the nature of immune responses induced in mice by chemicals may permit not only assessment of allergenic potential, but also prediction of the form allergic reactions will take.
Kimber
I
I
ICI Central Toxicology Laboratory, Cheshire, England, U.K.
Dearman
R J
RJ
D000485:Allergens / Q000032:analysis* / Q000145:classification; D000818:Animals; D003877:Dermatitis, Contact / Q000175:diagnosis*; D004342:Drug Hypersensitivity / Q000175:diagnosis*; D051379:Mice; D011237:Predictive Value of Tests; D012130:Respiratory Hypersensitivity / Q000139:chemically induced* / Q000175:diagnosis
D000485:Allergens