title: The distribution and properties of some hydrolytic enzymes from Porphyromonas gingivalis W50.
abstract: The production, distribution (as cell-bound, extracellular or associated with vesicles) and properties of four hydrolytic enzymes from Porphyromonas gingivalis were studied. During batch culture, enzymes appeared as cell bound until 72 h when extracellular trypsin-like protease and glycylprolyl dipeptidase were found. Alkaline phosphatase and N-acetyl-beta-glucosaminidase continued to be mainly cell-bound until 96 and 144 h post-inoculation, respectively. The pH optima for trypsin-like protease and dipeptidase activities were 7.5 and 8.0, respectively. Activity was most stable between pH 6.5 and 9.0 for the trypsin-like protease and pH 8.0 for dipeptidase. For phosphatase and N-acetyl-beta-glucosaminidase, double peaks of activity occurred at pH 8.0, 10.5 and 6.5, 7.5, respectively. Phosphatase was most stable at pH 7.0, whilst N-acetyl-beta-glucosaminidase was stable over a wide range of pH from 5.0-10.0. Molecular weight estimation by gel filtration gave 170 and 65 kD for trypsin-like protease, 200 and 24 kD for glycylprolyl dipeptidase, 125 and 24 kD for phosphatase, and 22 kD for N-acetyl-beta-glucosaminidase.
authors: Department of Basic Dental Science, University of Wales, Dental Hospital, Heath Park, Cardiff, Great Britain.
authors: A J
authors: AJ
authors: Smith
authors: T
authors: T
authors: Minhas
authors: J
authors: J
authors: Greenman
authors: G
authors: G
authors: Embery
mesh_terms: D000118:Acetylglucosaminidase / Q000096:biosynthesis* / Q000737:chemistry; D000469:Alkaline Phosphatase / Q000096:biosynthesis* / Q000737:chemistry; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases / Q000096:biosynthesis* / Q000737:chemistry; D010450:Endopeptidases / Q000096:biosynthesis* / Q000737:chemistry; D006089:Gram-Negative Anaerobic Bacteria / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration
chemical_list: D000469:Alkaline Phosphatase; D000118:Acetylglucosaminidase; D010450:Endopeptidases; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases; C059607:glycylprolyl dipeptidase