The sub-cellular localization of annexin V in cultured chick-embryo fibroblasts.
The Ca(2+)- and phospholipid-binding protein, annexin V, has been shown by an immune assay to represent 0.4% of total cell protein in cultured chick-embryo fibroblasts. Immunofluorescent localization studies indicate that in primary cultures the protein is abundant in the cytoplasm of the cells and also extends into the nucleus. Nuclear staining is no longer detectable, however, in approx. 25% of the cells following sub-culture. Sub-populations of annexin V are associated with cytoskeletal structures and with the inner face of the plasma membrane in a Ca(2+)-independent manner. In addition, we report results indicating the secretion of annexin V from this cell type.
Koster
J J
JJ
Department of Biochemistry and Molecular Biology, University of Leeds, U.K.
Boustead
C M
CM
Middleton
C A
CA
Walker
J H
JH
D000818:Animals; D017304:Annexin A5 / Q000032:analysis* / Q000378:metabolism; D002118:Calcium / Q000494:pharmacology; D002462:Cell Membrane / Q000737:chemistry; D002467:Cell Nucleus / Q000737:chemistry; D002478:Cells, Cultured; D002642:Chick Embryo; D003599:Cytoskeleton / Q000737:chemistry; D005347:Fibroblasts / Q000737:chemistry* / Q000648:ultrastructure*; D005455:Fluorescent Antibody Technique; D015151:Immunoblotting
D017304:Annexin A5; D002118:Calcium