Evaluation of a fully mechanised immunoassay--Enzymun-Test System ES 300--and comparison with in-house methods for 8 analytes.
The following analytes were determined with the automated enzyme immunoassay unit, Enzymun-Test System ES 300, and by routine in-house tests, and the results were compared: thyrotropin, free thyroxine, cortisol, immunoglobulin E, digoxin, digitoxin, insulin and carcinoembryonic antigen. The methods used for comparison included two radioimmunoassays, one with enzyme labelling, two with fluorescence enhanced enzyme reactions and 3 luminescence immunoassays. In most cases, the precision of the ES 300 lay between 2 and 5% in all assays in the concentration ranges of clinical interest. The inter-assay variation was almost identical to the intra-assay precision, which reflects the constancy of reagent quality and machine performance. The correlation between in-house and Enzymun-Test was excellent, even though the slope of the regression line was sometimes far from unity, due to the calibration materials used. ES 300 can be used as a stand alone unit and connected to a host computer. As a "walk-away" machine, it is suitable for laboratories with medium length series. It is a multi-batch analyser capable of taking 12 analytes and a maximal series length of 136 tubes excluding standards and controls.
Bauernfeind
M
M
Immunodiagnostic Evaluation Dept., Boehringer Mannheim, Germany.
Wood
W G
WG
D001774:Blood Chemical Analysis / Q000379:methods*; D002272:Carcinoembryonic Antigen / Q000097:blood; D004074:Digitoxin / Q000097:blood; D004077:Digoxin / Q000097:blood; D005069:Evaluation Studies as Topic; D006801:Humans; D006854:Hydrocortisone / Q000097:blood; D007118:Immunoassay; D007124:Immunoenzyme Techniques; D007073:Immunoglobulin E / Q000097:blood; D007328:Insulin / Q000097:blood; D008163:Luminescent Measurements; D011863:Radioimmunoassay; D012044:Regression Analysis; D015203:Reproducibility of Results; D013972:Thyrotropin / Q000097:blood; D013974:Thyroxine / Q000097:blood
D002272:Carcinoembryonic Antigen; D007328:Insulin; D007073:Immunoglobulin E; D004077:Digoxin; D013972:Thyrotropin; D004074:Digitoxin; D013974:Thyroxine; D006854:Hydrocortisone