Regional differences in sulfated oligosaccharides of rat gastrointestinal mucin as detected by two-dimensional chromatography.
Radiolabeled sulfated mucin was obtained from rat gastrointestinal tract tissue (corpus, antrum, duodenum, jejunum, ileum, or colon) incubated with [35S]sulfate in vitro by fractionation of extracts on Superose 6 followed by purification with CsCl density gradient centrifugation. Significant amounts of 35S-labeled mucin were obtained from each tissue and radioactivity differed according to region, in the order colon > duodenum > jejunum > corpus > antrum > ileum. Gel-permeation chromatography of 35S-labeled oligosaccharides liberated from each mucin by alkaline-borohydrate treatment indicated the 35S-labeled oligosaccharides of each mucin differ with respect to elution position. Further examination of fractions eluted from the gel-permeation column by thin-layer chromatography showed region-specific chromatograms for the sulfated oligosaccharides. Thus, not only the degree of sulfate incorporation but sulfated oligosaccharide structure differs according to the region in gastrointestinal mucin.
Goso
Y
Y
Department of Biochemistry, Kitasato University School of Medicine, Sagamihara, Japan.
Hotta
K
K
D000818:Animals; D002499:Centrifugation, Density Gradient; D002850:Chromatography, Gel; D002855:Chromatography, Thin Layer; D003106:Colon / Q000737:chemistry; D004064:Digestive System / Q000737:chemistry* / Q000378:metabolism; D004386:Duodenum / Q000737:chemistry; D007082:Ileum / Q000737:chemistry; D007583:Jejunum / Q000737:chemistry; D008297:Male; D015394:Molecular Structure; D009077:Mucins / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D009844:Oligosaccharides / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013270:Stomach / Q000737:chemistry; D013431:Sulfates / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D013462:Sulfur Radioisotopes; D014018:Tissue Distribution
D009077:Mucins; D009844:Oligosaccharides; D013431:Sulfates; D013462:Sulfur Radioisotopes