[The embryonic brain-derived neuralizing factor (EBDNF): its partial purification by ion-exchange and affinity chromatography].
The data are presented about isolation from the brain of 7.5-day chick embryos of a factor capable of neuralizing effect on the early gastrula ectoderm of the grass frog Rana temporaria L. Earlier this factor was defined as embryonic brain-derived neuralizing factor (EBDNF) (Mikhailov, Gorgoliuk, 1989). The isolation procedure includes (1) extraction with deionized water at pH 9.0; (2) ion exchange chromatography on a column with DEAE-adsorbent at pII 8.0; (3) affinity chromatography on Heparin-Ultragel column. EBDNF-containing fraction is eluted from the Heparin-Ultragel column with 250 mM NaCl as a separate peak. Four bands are observed on SDS-electrophoregrams of this fraction, two more prominent ones having the molecular weight of 43 and 63 kDa. The yield of EBDNF-containing fraction is about 0.01-0.02% of the wet weight of the initial brain tissue.
Mikhaĭlov
A T
AT
Gorgoliuk
N A
NA
Mukhoian
M M
MM
D000818:Animals; D016010:Binomial Distribution; D001921:Brain / Q000196:embryology*; D002642:Chick Embryo; D002846:Chromatography, Affinity / Q000379:methods / Q000706:statistics & numerical data; D002852:Chromatography, Ion Exchange / Q000379:methods / Q000706:statistics & numerical data; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods / Q000706:statistics & numerical data; D005775:Gastrula / Q000187:drug effects; D008970:Molecular Weight; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000302:isolation & purification* / Q000494:pharmacology; D011896:Rana temporaria
D009419:Nerve Tissue Proteins
Neĭralizuiushchiĭ faktor iz émbrional'nogo golovnogo mozga (EBDNF): chastichnaia ochistka s pomoshch'iu ionoobmennoĭ i affinnoĭ khromatografii.