The secondary structure of the 5'-noncoding region of beet necrotic yellow vein virus RNA 3: evidence for a role in viral RNA replication.
Secondary structure-sensitive chemical and enzymatic probes have been used to produce a model for the folding of the first 312 residues of the long 5'-noncoding region of beet necrotic yellow vein virus RNA 3. The structure consists of two major domains, one of which includes long distance base-pairing interactions between two short sequence elements (Box I and Box II) situated between positions 237 and 292 and complementary elements (Box I' and II') near the 5'-terminus. Previous studies have shown that base pairing between these sequence elements (in either the plus-strand or minus-strand RNA) is important for RNA 3 accumulation during infection. RNA 3 transcripts were produced containing mutations which preferentially disrupted Box II-II' base pairing in either the plus- or minus-strand. In infection experiments, transcripts with mutations which disrupted the Box II-II' interaction in the plus-strand structure replicated less efficiently than mutants in which the Box II-II' interaction was disrupted in the minus-strand. These findings indicate that the complex 5'-proximal plus-strand structure to which the Box II-II' interaction contributes comprises at least part of the promoter for plus-strand RNA synthesis.
Gilmer
D
D
Institut de Biologie Moléculaire des Plantes du CNRS, Strasbourg, France.
Allmang
C
C
Ehresmann
C
C
Guilley
H
H
Richards
K
K
Jonard
G
G
Ehresmann
B
B
D001483:Base Sequence; D015870:Gene Expression; D006860:Hydrogen Bonding; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009690:Nucleic Acid Conformation; D010942:Plant Viruses / Q000235:genetics* / Q000648:ultrastructure; D011401:Promoter Regions, Genetic; D012328:RNA Viruses / Q000235:genetics* / Q000648:ultrastructure; D012333:RNA, Messenger / Q000235:genetics; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics* / Q000648:ultrastructure; D014675:Vegetables; D014779:Virus Replication
D012333:RNA, Messenger; D012367:RNA, Viral