Changes in the retinal ganglion cell layer and optic nerve of rats exposed neonatally to cocaine.
The effects of neonatal exposure to cocaine upon the structure of the visual system are poorly understood despite the evidence of eye abnormalities in infants exposed in utero to cocaine. We previously demonstrated alterations in the optic nerve of rats exposed neonatally to cocaine, although no changes were detected in the number of its axons. This study was undertaken to investigate the retinal ganglion cell layer and the size distribution of the optic axons, in an attempt to assess further changes in the visual pathways. Groups of rats (Wistar strain) were given subcutaneous injections of cocaine hydrochloride (15 mg kg-1 body weight day-1) divided into two daily doses, from the day following birth until postnatal day 30 Controls were given subcutaneous saline throughout the same experimental period. Per group, five animals from three different litters were evaluated morphometrically. Following perfusion with aldehydes, samples from the median ventral and dorsal parts of the retina and the optic nerves were processed for electron microscopy. Morphometric techniques at light and electron microscopic levels allowed us to determine the following. (a) In the optic nerve: (1) frequency size-distribution of myelinated nerve fibres; and (2) number of myelin sheaths per fibre. (b) In the retina: (1) thickness of the layers; (2) frequency size-distribution of ganglion layer neurons; (3) mean cell nuclear size; and (4) packing density of ganglion cell layer neurons.(ABSTRACT TRUNCATED AT 250 WORDS)
Silva-Araújo
A
A
Department of Ophthalmology, Medical School of Porto, Portugal.
Salgado-Borges
J
J
Cardoso
V
V
Silva
M C
MC
Castro-Correia
J
J
Tavares
M A
MA
D000818:Animals; D000831:Animals, Newborn; D001369:Axons / Q000648:ultrastructure; D001699:Biometry; D003042:Cocaine / Q000633:toxicity*; D008297:Male; D008854:Microscopy, Electron; D009413:Nerve Fibers, Myelinated / Q000648:ultrastructure; D009900:Optic Nerve / Q000187:drug effects* / Q000648:ultrastructure; D051381:Rats; D017208:Rats, Wistar; D012165:Retinal Ganglion Cells / Q000187:drug effects* / Q000648:ultrastructure
D003042:Cocaine