title: [Isolation and characteristics of catalytic antibodies to DNA in systemic lupus erythematosis].
abstract: A method for purifying DNA-specific catalytic antibodies based on affinity chromatography on protein A Sepharose and on both modified and non-modified DNA-cellulose as well as HPLC has been developed. The elution conditions with high yields of DNA-hydrolyzing activity of antibodies have been optimized. The biochemical and immunological properties of catalytic antibodies have been examined. The kinetic parameters of the enzyme interaction with an oligonucleotide substrate have been determined. The influence of effectors on DNA hydrolysis by antibodies has been investigated.
authors: G V
authors: GV
authors: Gololobov
authors: A E
authors: AE
authors: Bogomolova
authors: R P
authors: RP
authors: Iadav
authors: M V
authors: MV
authors: Ermolaeva
authors: K M
authors: KM
authors: Belostotskaia
authors: T B
authors: TB
authors: Prokaeva
authors: A M
authors: AM
authors: Shuster
authors: A G
authors: AG
authors: Gabibov
mesh_terms: D000974:Antibodies, Antinuclear / Q000276:immunology / Q000302:isolation & purification*; D017151:Antibodies, Catalytic / Q000276:immunology / Q000302:isolation & purification*; D001483:Base Sequence; D001666:Binding Sites, Antibody; D002846:Chromatography, Affinity; D006801:Humans; D006868:Hydrolysis; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*; D008969:Molecular Sequence Data
chemical_list: D000974:Antibodies, Antinuclear; D017151:Antibodies, Catalytic
vernacular_title: Vydelenie i kharakteristika kataliticheskikh antitel k DNK pri sistemnoĭ krasnoĭ volchanke.