A 19F NMR study of the interaction of 3-fluoro-4-demethoxydaunomycin with the hexanucleotide d(TCCGGA)2.
19F NMR spectroscopy has been used to study the binding of the fluorinated anthracycline 3-fluoro-4-demethoxydaunomycin with the hexanucleotide d(TCCGGA)2. In the spectrum of the 1:1 anthracycline-d(TCCGGA)2 complex four resonances of approximately equal intensity were observed. This indicated that 4-demethoxydaunomycin intercalated at all possible sites with similar affinity. This suggests that the specific high affinity binding sites that are observed in anthracycline-DNA footprinting experiments are strongly regulated by the local DNA conformation.
Barr
K E
KE
Department of Chemistry, University College, University of New South Wales, Australia.
Russell
R A
RA
Warrener
R N
RN
Collins
J G
JG
D001483:Base Sequence; D003630:Daunorubicin / Q000031:analogs & derivatives* / Q000378:metabolism; D005461:Fluorine; D007364:Intercalating Agents / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D009838:Oligodeoxyribonucleotides / Q000378:metabolism*
D007364:Intercalating Agents; D009838:Oligodeoxyribonucleotides; C065327:3-fluoro-4-demethoxydaunomycin; D005461:Fluorine; D003630:Daunorubicin