The role of ALA-S and ALA-D in regulating porphyrin biosynthesis in a normal and a HEM R+ mutant strain of Saccharomyces cerevisiae.
Catabolite repression and derepression on delta-aminolevulinate synthase (ALA-S) and delta-aminolevulinate dehydratase (ALA-D) in a normal yeast strain, D27, and its derived D27/C6 (HEM R+) were investigated. ALA-S and ALA-D activities and intracellular ALA (I-ALA) at different physiological states of the cells were measured. In YPD medium, under conditions of repression and when glucose was exhausted, both strains behaved identically as if the mutation was not expressed. In YPEt medium, however, both ALA-S and ALA-D activities were higher than in YPD, but the I-ALA content and the enzymic activity profiles shown by the two strains were quite different. It appears, therefore, that the mutation causes a deregulation of ALA-S, so that its activity is kept at a high level throughout the cell cycle. This would explain the increased levels of cytochromes present in the mutant. This mutation may affect some regulatory aspect of ALA formation and renders an ALA-S of high activity; moreover, this enzyme species seems to be more stable than in the normal strain.
Correa García
S
S
Centro de Investigaciones sobre Porfirinas y Porfirias, CIPYP (CONICET, F.C.E. y N., UBA), Ciudad Universitaria, Argentine.
Bermúdez Moretti
M
M
Cardalda
C
C
Rossetti
M V
MV
Batlle
A M
AM
D000624:5-Aminolevulinate Synthetase / Q000378:metabolism*; D003580:Cytochromes / Q000096:biosynthesis; D004794:Enzyme Repression; D011163:Hydroxymethylbilane Synthase / Q000032:analysis; D009154:Mutation; D000623:Porphobilinogen Synthase / Q000378:metabolism*; D011166:Porphyrins / Q000096:biosynthesis*; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D013053:Spectrophotometry; D014575:Uroporphyrinogen Decarboxylase / Q000032:analysis
D003580:Cytochromes; D011166:Porphyrins; D000624:5-Aminolevulinate Synthetase; D011163:Hydroxymethylbilane Synthase; D014575:Uroporphyrinogen Decarboxylase; D000623:Porphobilinogen Synthase