Monoclonal antibody enhancement of FSH-induced uterine growth in snell dwarf mice.
The enhancing effect of bovine FSH monoclonal antibody (bFSH-MAb) on the gonadotrophic activity of FSH was investigated in dwarf mice using a uterine weight bioassay. Increasing doses of bovine FSH (NIH-FSH-B1; 3.3, 10 or 30 micrograms/day) were administered for 5 days to dwarf mice (groups of five) with or without administration of a bFSH-MAb preparation (USDA-bFSH-MC28; 100 micrograms protein/day) which at a dilution of 1:15,000 bound 50% of 125I-labelled bFSH (USDA-bFSH-BP3). The bFSH, at the doses given, gave no increases in uterine weight; when, however, these doses were given with bFSH-MAb, significant (three- to four fold) increases in uterine weight resulted.
Glencross
R G
RG
Department of Biochemistry and Physiology, University of Reading, Whiteknights, U.K.
Lovell
R D
RD
Holder
A T
AT
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000494:pharmacology*; D002417:Cattle; D004393:Dwarfism, Pituitary / Q000503:physiopathology*; D005260:Female; D005640:Follicle Stimulating Hormone / Q000276:immunology* / Q000494:pharmacology; D051379:Mice; D008817:Mice, Mutant Strains; D009929:Organ Size / Q000187:drug effects; D014599:Uterus / Q000033:anatomy & histology / Q000187:drug effects / Q000254:growth & development*
D000911:Antibodies, Monoclonal; D005640:Follicle Stimulating Hormone