title: Human fetal ovaries and uteri: developmental expression of genes encoding the insulin, insulin-like growth factor I, and insulin-like growth factor II receptors.
abstract: OBJECTIVE
  To study the messenger ribonucleic acid (mRNA) expression of receptors for insulin, insulin-like growth factor I (IGF-I), and insulin-like growth factor II (IGF-II) in human fetal ovaries and uteri at 10, 15, 19, and 22 weeks' gestation. Insulin, IGF-I, and IGF-II are postulated to have paracrine and autocrine functions in the development of fetal tissues, but receptors for these growth factors have never been studied in reproductive organs of the human female fetus.
  
  
  METHODS
  Ribonucleic acid extracted from human fetal reproductive tissues underwent reverse transcription and polymerase chain reaction (PCR) amplification. The amplified complementary deoxyribonucleic acid (cDNA) fragments were analyzed by gel electrophoresis and restriction enzyme mapping.
  
  
  RESULTS
  Messenger RNA expression consistent with both known forms of the insulin receptor, the IGF-I receptor, and the IGF-II receptor was observed in human fetal ovaries and uteri at 10, 15, 19, and 22 weeks' gestation. The identity of the PCR amplified ovarian and uterine DNA fragments were confirmed by digestion with the restriction endonucleases AvaII, DdeI, and BamHI, respectively.
  
  
  CONCLUSIONS
  This study demonstrates that mRNA for receptors for insulin, IGF-I, and IGF-II are expressed in the fetal human female genital tract. These receptors may have roles in fetal reproductive tract development.
authors: Department of Obstetrics and Gynecology, Brigham and Women's Hospital, Boston, Massachusetts 02115.
authors: J L
authors: JL
authors: Shifren
authors: R
authors: R
authors: Osathanondh
authors: J
authors: J
authors: Yeh
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D005314:Embryonic and Fetal Development; D005260:Female; D005786:Gene Expression Regulation; D005865:Gestational Age; D006801:Humans; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D010053:Ovary / Q000196:embryology / Q000502:physiology*; D016133:Polymerase Chain Reaction / Q000379:methods; D011247:Pregnancy; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification; D017526:Receptor, IGF Type 1 / Q000235:genetics*; D017527:Receptor, IGF Type 2 / Q000235:genetics*; D011972:Receptor, Insulin / Q000235:genetics*; D015183:Restriction Mapping; D014599:Uterus / Q000196:embryology / Q000502:physiology*
chemical_list: D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger; D017527:Receptor, IGF Type 2; D004247:DNA; D017526:Receptor, IGF Type 1; D011972:Receptor, Insulin