[In vitro suppression of translation of tick-borne encephalitis virus RNA using antisense nucleotides].
Effect of antisense oligodeoxyribonucleotides on in vitro translation of RNAs corresponding to fragments of the tick-borne encephalitis virus genome has been investigated. Sequences optimal for oligonucleotide binding and translation arrest have been identified. The most efficient oligonucleotide (17-mer) at a concentration of 2.5 microM completely arrests translation of the RNA coding for the NS3 protein.
Nomokonova
N Iu
NIu
Gorn
V V
VV
Vlasov
V V
VV
D001483:Base Sequence; D004669:Encephalitis Viruses, Tick-Borne / Q000235:genetics*; D008969:Molecular Sequence Data; D016376:Oligonucleotides, Antisense / Q000494:pharmacology*; D014176:Protein Biosynthesis; D020365:RNA Helicases; D012333:RNA, Messenger / Q000235:genetics; D012367:RNA, Viral / Q000187:drug effects / Q000235:genetics*; D012697:Serine Endopeptidases; D017361:Viral Nonstructural Proteins / Q000235:genetics; D043583:Nucleoside-Triphosphatase; D053487:DEAD-box RNA Helicases; D000085242:Viral Proteases
D016376:Oligonucleotides, Antisense; D020365:RNA Helicases; D012333:RNA, Messenger; D012367:RNA, Viral; D012697:Serine Endopeptidases; D017361:Viral Nonstructural Proteins; C060028:NS3 protein, flavivirus; D043583:Nucleoside-Triphosphatase; D053487:DEAD-box RNA Helicases; D000085242:Viral Proteases
Podavlenie transliatsii in vitro fragmentov RNK virusa kleshchevogo éntsefalita s pomoshch'iu antismyslovykh nukleotidov.