title: The recombinant catalytic domain of human neutrophil collagenase lacks type I collagen substrate specificity.
abstract: The coding region for human neutrophil short form procollagenase lacking the hemopexin like domain coding region was amplified by polymerase chain reaction. Recombinant short form procollagenase was expressed in E. coli and purified in a three step procedure. Renaturation of this proenzyme was carried out by an effective new method using Q-Sepharose chromatography. Treatment of short form procollagenase with mercurials resulted in active short form collagenase M(r) 21,000 and an intermediate product of M(r) 23,000. These two products were separated by hydroxamate affinity chromatography. The active, short form collagenase M(r) 21,000 is stable. Despite full proteolytic activity, it lacks type I collagen substrate specificity and forms the basis for crystallisation experiments.
authors: Department of Biochemistry, Faculty of Chemistry, University of Bielefeld, Germany.
authors: S
authors: S
authors: Schnierer
authors: T
authors: T
authors: Kleine
authors: T
authors: T
authors: Gote
authors: A
authors: A
authors: Hillemann
authors: V
authors: V
authors: Knäuper
authors: H
authors: H
authors: Tschesche
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003094:Collagen / Q000378:metabolism*; D017364:Collagenases / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D004247:DNA; D004792:Enzyme Precursors / Q000235:genetics / Q000302:isolation & purification; D004926:Escherichia coli; D006801:Humans; D008969:Molecular Sequence Data; D009504:Neutrophils / Q000201:enzymology*; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D013379:Substrate Specificity
chemical_list: D004792:Enzyme Precursors; D011994:Recombinant Proteins; D003094:Collagen; D004247:DNA; D017364:Collagenases; C022440:procollagenase