title: Magnetic resonance imaging and morphometric quantitation of cartilage histology after chronic infusion of interleukin 1 in rabbit knees.
abstract: Cartilage pathology in rabbit knees was monitored by noninvasive magnetic resonance imaging (MRI) and evaluated using morphometric histologic measurements. Infusion of rabbit knees with the cytokine interleukin 1 induces cartilage degradation and inflammation. A miniosmotic pump was implanted subcutaneously to deliver interleukin 1 through a polyethylene catheter inserted into the rabbit knee. Rabbit knees were imaged using MRI and prepared for histologic examination at 5 and 12 days after chronic infusion of interleukin 1. MRI obtained 0.7-mm sections for three-dimensional reconstruction of cartilage image. Cartilage deterioration near the site of infusion was visible on MRI. MRI measurements indicated a reduction in cartilage thickness. Histology revealed a loss of staining of cartilage matrix proteoglycan, synovial hypertrophy, and perichondral bone resorption. Morphometric analysis of cartilage histology indicated a reduction in both cellularity (chondrocytes/m mu 2 area) and cell to matrix area ratio. These observations suggest that a loss of proteoglycan, an early event in cartilage degeneration, can be detected by MRI.
authors: Research Department, Ciba-Geigy Corp., Summit, New Jersey 07901.
authors: D
authors: D
authors: Wilson
authors: P K
authors: PK
authors: Paul
authors: E D
authors: ED
authors: Roberts
authors: V
authors: V
authors: Blancuzzi
authors: J
authors: J
authors: Gronlund-Jacob
authors: K
authors: K
authors: Vosbeck
authors: G
authors: G
authors: DiPasquale
authors: E M
authors: EM
authors: O'Byrne
mesh_terms: D000818:Animals; D002358:Cartilage, Articular / Q000187:drug effects / Q000473:pathology*; D006801:Humans; D007263:Infusions, Parenteral; D007375:Interleukin-1 / Q000008:administration & dosage / Q000633:toxicity*; D008279:Magnetic Resonance Imaging / Q000379:methods; D008297:Male; D011817:Rabbits; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000633:toxicity; D013583:Synovial Membrane / Q000187:drug effects / Q000473:pathology*
chemical_list: D007375:Interleukin-1; D011994:Recombinant Proteins