A new method for isolation of the intraerythrocytic stages of Plasmodium and Babesia from their host cells.
A new method for the isolation of intraerythrocytic stages of Plasmodium berghei and Babesia divergens from red blood cells is described. The technique is based on hydrodynamic forces occurring in a flow channel containing a turbulent liquid current, which are capable of rupturing infected erythrocytes and removing their plasma membrane from the parasites' surface. The temperature and the concentration of cells were revealed as factors influencing the hydrodynamic forces. About 90% of the intact and apparently infectious parasites of both species were isolated from the lysed erythrocytes.
Sobolewski
B
B
Lehrstuhl für Spezielle Zoologie und Parasitologie, Ruhr-Universität Bochum, Germany.
Mackenstedt
U
U
Mehlhorn
H
H
D000818:Animals; D001403:Babesia / Q000302:isolation & purification* / Q000648:ultrastructure; D002458:Cell Fractionation / Q000379:methods*; D004912:Erythrocytes / Q000166:cytology / Q000469:parasitology*; D005849:Gerbillinae / Q000469:parasitology; D008722:Methods; D051379:Mice; D008807:Mice, Inbred BALB C / Q000469:parasitology; D008854:Microscopy, Electron; D010962:Plasmodium berghei / Q000302:isolation & purification* / Q000648:ultrastructure