Nucleotide sequence of the gene coding for Clostridium barati type F neurotoxin: comparison with other clostridial neurotoxins.
The neurotoxin gene from Clostridium barati ATCC43756 was cloned as a series of overlapping polymerase chain reaction (PCR) generated fragments using primers designed to conserve toxin sequences previously published. The toxin gene has an open reading frame (ORF) of 1268 amino acids giving a calculated molecular mass of 141,049 Da. The sequence identity between the C. barati ATCC43756 and non-proteolytic C. botulinum 202F neurotoxins is 64.2% for the light chain and 73.6% for the heavy chain. This is much lower than reported identities for the type E neurotoxins from C. botulinum and C. butyricum (96% identity between light chains and 98.8% between the heavy chains). Previously identified conserved regions in other botulinal neurotoxins were also conserved in that of C. barati. An ORF upstream of the toxin coding region was revealed. This shows strong homology to the 3' end of the gene coding for the nontoxic-nonhemagglutinin (NTNH) component of the progenitor toxin from C. botulinum type C neurotoxin.
Thompson
D E
DE
Department of Microbiology, AFRC Institute of Food Research, Reading Laboratory, UK.
Hutson
R A
RA
East
A K
AK
Allaway
D
D
Collins
M D
MD
Richardson
P T
PT
D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D001427:Bacterial Toxins / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D003013:Clostridium / Q000235:genetics*; D017124:Conserved Sequence; D005798:Genes, Bacterial / Q000235:genetics*; D014644:Genetic Variation; D008969:Molecular Sequence Data; D009498:Neurotoxins / Q000235:genetics*; D016133:Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D013744:Tetanus Toxin / Q000145:classification / Q000235:genetics*
D001426:Bacterial Proteins; D001427:Bacterial Toxins; C081095:Clostridium barati F toxin; D009498:Neurotoxins; D013744:Tetanus Toxin