Recombinant f1 phage particles can transfect monkey COS-7 cells by DEAE dextran method.
Recombinant f1 phage particles containing cDNA of the human urokinase type-plasminogen activator (u-PA) under the control of the simian virus 40 (SV40) early promoter were used for transfection of monkey COS-7 cells using a DEAE dextran method. The fibrinolytic activity of u-PA was detected in the culture medium of the 10(5) cells transfected with the phage particles containing single-stranded (ss) DNA of more than 0.2 ng. This finding will lead us to develop a simple and efficient method for expression cloning using mammalian cells.
Yokoyama-Kobayashi
M
M
Kanagawa Academy of Science and Technology, Japan.
Kato
S
S
D000818:Animals; D001435:Bacteriophages / Q000235:genetics*; D001483:Base Sequence; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D003637:DEAE-Dextran; D004274:DNA, Recombinant; D006801:Humans; D008969:Molecular Sequence Data; D010957:Plasmids; D011995:Recombination, Genetic; D014162:Transfection / Q000379:methods*; D014568:Urokinase-Type Plasminogen Activator / Q000235:genetics
D004274:DNA, Recombinant; D003637:DEAE-Dextran; D014568:Urokinase-Type Plasminogen Activator