Study of intracellular deposition of the anti-leprosy drug clofazimine in mouse spleen using laser microprobe mass analysis.
Laser microprobe mass analysis (LAMMA) was used to study the composition of the brick-red crystalline material which had accumulated in the spleen of mice that had received the anti-leprosy drug Clofazimine in their diet for several months. The crystalline deposits light-microscopically resembled pure Clofazimine crystals. The presence of the drug in the crystals was indicated by LAMMA by the appearance of the chloride mass peaks in the negative mass spectra. More specific information was obtained from the positive mass spectra. A mass signal for the protonated molecule was present.
Vandeputte
D
D
Department of Chemistry, University of Antwerp (UIA), Antwerp-Wilrijk, Belgium.
Jacob
W
W
Van Grieken
R
R
Boddingius
J
J
D000818:Animals; D002991:Clofazimine / Q000032:analysis / Q000493:pharmacokinetics*; D003460:Crystallization; D004032:Diet; D005260:Female; D007834:Lasers; D013058:Mass Spectrometry; D051379:Mice; D008808:Mice, Inbred CBA; D013154:Spleen / Q000737:chemistry / Q000166:cytology / Q000378:metabolism*
D002991:Clofazimine