title: Porphyrin synthesis by murine epidermal cells.
abstract: Incubation of murine epidermal cells with delta-aminolevulinic acid (ALA) resulted in a dose- and time-dependent accumulation of porphyrins, predominantly of protoporphyrin. Porphyrin accumulation decreased in the presence of iron, and the iron-mediated decrease was partially reversed by CaMg EDTA (1.25-10.0 mM), suggesting that there is functionally active ferrochelatase in these cells. This study suggests that these cells may be a useful model for the study of cutaneous porphyrin metabolism involving ferrochelatase activity.
authors: Dermatology Service, Department of Veterans Affairs Medical Center, New York, NY 10010.
authors: D
authors: D
authors: He
authors: E
authors: E
authors: Karas
authors: S
authors: S
authors: Sassa
authors: H W
authors: HW
authors: Lim
mesh_terms: D000622:Aminolevulinic Acid / Q000494:pharmacology; D000818:Animals; D002470:Cell Survival; D002478:Cells, Cultured; D004492:Edetic Acid / Q000494:pharmacology; D000078404:Epidermal Cells; D004817:Epidermis / Q000378:metabolism; D005260:Female; D005294:Ferrochelatase / Q000502:physiology; D007501:Iron / Q000494:pharmacology; D007700:Kinetics; D051379:Mice; D008812:Mice, Hairless; D008954:Models, Biological; D011166:Porphyrins / Q000096:biosynthesis*; D012867:Skin / Q000166:cytology / Q000378:metabolism*
chemical_list: D011166:Porphyrins; D000622:Aminolevulinic Acid; D004492:Edetic Acid; D007501:Iron; D005294:Ferrochelatase