title: Neutrophil adhesion to endothelial cells impairs the effects of catalase and glutathione in preventing endothelial injury.
abstract: We studied the effects of the CuZn superoxide dismutase (SOD), catalase (CAT), and glutathione (GSH) on endothelial permeability to 125I-albumin after activation of neutrophils (PMN) with phorbol 12-myristate-13-acetate (PMA; 10(-8) M). PMN were either in direct contact with the endothelial cell monolayer grown on a porous gelatin-coated microporous 10-microns-thick polycarbonate filter (upright system) or separated from the endothelium by a similar filter (inverted system). Transendothelial 125I-albumin clearance rates were measured as an index of endothelial permeability. In the absence of antioxidants, activation of PMN increased transendothelial 125I-albumin clearance rates in both systems from 0.041 +/- 0.006 microliters/min (baseline) to 0.262 +/- 0.18 microliters/min (upright system) and from 0.063 +/- 0.02 microliters/min to 0.244 +/- 0.06 microliters/min (inverted system). PMA induced 80-90% of PMN to adhere to either gelatin-coated filters or to endothelial cells, from the basal PMN adhesion value of 5.3 +/- 2.2% and 4.3 +/- 1.1%, respectively. SOD, which dismutases superoxide anion to hydrogen peroxide (H2O2), did not alter the transendothelial 125I-albumin clearance rates in either system at any concentration from 10-300 U/ml. CAT (100-1,000 U/ml) and GSH (0.5-10 mM), which remove the H2O2 generated during PMN activation, did not alter the increase in transendothelial 125I-clearance rates after PMN activation in the upright system, but both agents prevented the increase in transendothelial 125I-clearance rates in the inverted system. We conclude that PMN activation with PMA causes endothelial injury irrespective of PMN contact to the endothelial monolayer. Moreover, H2O2, a release product of PMN activation, is a critical mediator of PMN-dependent endothelial injury. Finally, the results indicate that CAT and GSH prevent endothelial injury only in the absence of direct PMN contact with endothelial cells, suggesting that antioxidants such as GSH and CAT are excluded from sites of PMN-endothelial contact and thus are ineffective antioxidants.
authors: Department of Physiology and Cell Biology, Albany Medical College, New York 12208.
authors: A
authors: A
authors: Siflinger-Birnboim
authors: A B
authors: AB
authors: Malik
mesh_terms: D000818:Animals; D002374:Catalase / Q000494:pharmacology*; D002448:Cell Adhesion; D004730:Endothelium, Vascular / Q000166:cytology* / Q000187:drug effects / Q000473:pathology; D005978:Glutathione / Q000494:pharmacology*; D009504:Neutrophils / Q000502:physiology*; D013482:Superoxide Dismutase / Q000494:pharmacology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: D002374:Catalase; D013482:Superoxide Dismutase; D005978:Glutathione; D013755:Tetradecanoylphorbol Acetate