Modelling of three-dimensional fluorescence images of muscle fibres: an application of the three-dimensional optical transfer function.
Striated muscle fibres can be modelled by a simple geometry, which has allowed three-dimensional (3-D) images in conventional and confocal microscopes to be calculated. This model is useful for comparing different imaging methods and represents a simple example of an application of the 3-D optical transfer function (OTF) for the system. The rejection of out-of-focus blur is demonstrated, and the effects of fibre thickness and confocal pinhole size on image contrast are investigated. The effects of using a simple filter for image enhancement are studied, elucidating the characteristics of the OTF.
Sheppard
C J
CJ
Physical Optics Department, School of Physics, University of Sydney, N.S.W., Australia.
Gu
M
M
D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods; D008853:Microscopy / Q000379:methods*; D008954:Models, Biological; D009132:Muscles / Q000033:anatomy & histology*