The Ti plasmid from the wide host range Agrobacterium vitis strain Tm4: map and homology with other Ti plasmids.
The octopine/cucumopine (o/c) strains of Agrobacterium vitis are divided into limited (LHR) and wide host range (WHR) strains. We present here the first map of a Ti plasmid from a WHR o/c strain, Tm4. pTiTm4 (252 kb) has homology with the octopine Ti plasmid pTiAch5, the nopaline plasmid pTiC58, and the Ri plasmid pRiHRI. Approximately 155 kb of pTiTm4 is homologous with the LHR o/c Ti plasmids pTiAB3 and pTiAg57, the remaining part is pTiTm4-specific. Our data suggest that the evolution of the o/c Ti plasmids was accompanied by large-scale changes in plasmid structure. The origin of replication of pTiTm4 was localized by a functional assay.
Otten
L
L
C.N.R.S. Institute of Plant Molecular Biology, Strasbourg, France.
Gérard
J C
JC
De Ruffray
P
P
D005075:Biological Evolution; D002874:Chromosome Mapping; D010957:Plasmids / Q000235:genetics*; D015183:Restriction Mapping; D012231:Rhizobium / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid