Comparative studies of synthesis, phosphorylation, DNA binding and proteolytic characteristics of a novel protein during phases of the mouse spleen cell cycle.
1. Cultured mouse spleen cells were exposed to the mitogen Concanavalin A followed by isoproterenol, and nuclei were electronically sorted from seven partitions of the cell cycle. 2. Several nuclear proteins, including stress proteins, which were cell-cycle-stage specific, were elicited by isoproterenol as determined by micro-electrophoresis and fluorography. 3. Two novel S-phase proteins (X0 and X') demonstrated differing synthesis and phosphorylation patterns during the cell-cycle phases. 4. X' showed DNA binding characteristics and proteolytic properties (hydrolyzing X0 or beta-galactosidase); both proteins were cell-cycle regulated.
Pipkin
J L
JL
Department of Health and Human Services, Food and Drug Administration, National Center for Toxicological Research, Jefferson, AR 72079.
Anson
J F
JF
Hinson
W G
WG
Lyn-Cook
L E
LE
Schol
H M
HM
Burns
E R
ER
Feuers
R J
RJ
Casciano
D A
DA
Sheehan
D M
DM
D000818:Animals; D002453:Cell Cycle; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D004247:DNA / Q000378:metabolism*; D006868:Hydrolysis; D007545:Isoproterenol / Q000494:pharmacology; D008297:Male; D008715:Methionine / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D009687:Nuclear Proteins / Q000096:biosynthesis* / Q000378:metabolism; D010664:Phenylmethylsulfonyl Fluoride / Q000494:pharmacology; D010766:Phosphorylation; D011485:Protein Binding; D013154:Spleen / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*
D009687:Nuclear Proteins; D003208:Concanavalin A; D010664:Phenylmethylsulfonyl Fluoride; D004247:DNA; D008715:Methionine; D007545:Isoproterenol