Cloning of the rat cyclin-dependent kinase 4 cDNA: implication in proliferation-dependent expression in rat tissues.
To identify protein kinases that may regulate fetal growth and differentiation, we used an oligonucleotide probe encoding the conserved sequence of serine/threonine kinases to screen a fetal lung cDNA library. Several clones were isolated and sequenced, one of which encodes the rat homolog of the 34 kilodalton cyclin-dependent kinase 4 (p34cdk4). Northern blot analyses of pre- and postnatal rat tissues show that rat cdk4 is expressed in a developmentally regulated pattern in all tissues examined. The mRNA is also significantly decreased in cells that are arrested in the G1 phase of cell cycle. The regulated expression of rat cdk4 is consistent with a proliferation-, rather than a differentiation-, dependent pattern.
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University of Cincinnati College of Medicine, Department of Pediatrics, Ohio 45229.
Phillips
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Khan
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Weaver
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D000367:Age Factors; D000818:Animals; D001483:Base Sequence; D002453:Cell Cycle; D003001:Cloning, Molecular; D051358:Cyclin-Dependent Kinase 4; D018844:Cyclin-Dependent Kinases; D016213:Cyclins / Q000502:physiology; D015870:Gene Expression; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D011494:Protein Kinases / Q000235:genetics*; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D016415:Sequence Alignment
D016213:Cyclins; D009838:Oligodeoxyribonucleotides; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D011494:Protein Kinases; C495900:CDK4 protein, human; C495902:Cdk4 protein, mouse; C495903:Cdk4 protein, rat; D051358:Cyclin-Dependent Kinase 4; D018844:Cyclin-Dependent Kinases