title: Preliminary X-ray study of naproxen esterase from Bacillus subtilis.
abstract: Single crystals of naproxen esterase from Bacillus subtilis have been obtained from PEG6000 solutions at pH 8.0 by liquid-liquid diffusion while applying a temperature gradient from 4 degrees C to room temperature over a period of four weeks. The crystals belong to the trigonal space group P3(1)21 or P3(2)21 with a = b = 47.59 A and c = 212.91 A. The asymmetric part of the unit cell contains one protein molecule with M(r) = 33,771. The crystals diffract to at least 3.0 A resolution and are suitable for an X-ray structure analysis.
authors: Royal Gist-brocades N.V. Research and Development, Delft, The Netherlands.
authors: J M
authors: JM
authors: van der Laan
authors: A V
authors: AV
authors: Teplyakov
authors: A A
authors: AA
authors: Lammers
authors: B W
authors: BW
authors: Dijkstra
mesh_terms: D001412:Bacillus subtilis / Q000201:enzymology*; D043182:Carboxylesterase; D002265:Carboxylic Ester Hydrolases / Q000737:chemistry* / Q000302:isolation & purification; D003460:Crystallization; D011487:Protein Conformation; D014961:X-Ray Diffraction / Q000379:methods
chemical_list: D002265:Carboxylic Ester Hydrolases; D043182:Carboxylesterase