Difference in glutathione S-transferase response to oxidative stress between porcine and bovine lens.
Porcine and bovine lens GSTs were compared in the stability against various oxidative stress which is a major factor of cataract formation in order to clarify the role of lens glutathione S-transferase (GST) and its relation to cataractogenesis. Class pi porcine lens GST was inactivated reversibly by biological disulfides, cystine and cystamine, and also inactivated by active oxygen species such as O2- generated through xanthine-xanthine oxidase system and H2O2. On the other hand, class mu bovine lens GST was insensitive to such applied oxidative stress. Furthermore, 1,2-naphthoquinone, which is a metabolite of naphthalene and an actual inducer of naphthalene cataract, strongly inactivated porcine lens GST though it did not affect bovine enzyme. Thus, porcine and bovine lens GSTs had different sensitivity to various oxidative stress which could induce cataract formation. The results suggest that the differential expression of GST isozymes among animals may explain the variation in the cataract formation caused by oxidative stress.
Nishinaka
T
T
Laboratory of Biochemistry, Faculty of Pharmaceutical Sciences, Osaka University, Japan.
Terada
T
T
Nanjo
H
H
Mizoguchi
T
T
Nishihara
T
T
D000818:Animals; D002386:Cataract / Q000209:etiology; D002417:Cattle; D003538:Cystamine / Q000494:pharmacology; D003553:Cystine / Q000494:pharmacology; D005982:Glutathione Transferase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D007908:Lens, Crystalline / Q000187:drug effects / Q000201:enzymology*; D009285:Naphthoquinones / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000494:pharmacology*; D013552:Swine
D009285:Naphthoquinones; D017382:Reactive Oxygen Species; D003553:Cystine; C040756:1,2-naphthoquinone; D005982:Glutathione Transferase; D003538:Cystamine