title: Effects of donor characteristics and platelet in vitro time and temperature on platelet aggregometry.
abstract: OBJECTIVE
  This study was undertaken to determine whether platelet donor characteristics and the duration and temperature of platelet storage affect platelet aggregation.
  
  
  METHODS
  Half of each platelet-rich plasma sample, obtained from 42 healthy volunteers, was maintained at 37 degrees C and the other half at 25 degrees C. Aggregation was stimulated with adenosine diphosphate (ADP), epinephrine, or collagen 1, 2, 4, and 6 hours after platelet donation. The lag time, slope of the aggregation curve, and percent peak aggregation were determined for each aggregation test.
  
  
  RESULTS
  Age, sex, time of donation, smoking status, estrogen replacement, and menstruation did not significantly influence platelet aggregation. Storage time and temperature had a major influence on platelet reactivity, with the reactivity decreasing with prolonged storage and higher storage temperatures. Peak ADP-induced aggregation for platelets stored at 37 degrees C was significantly less (p < 0.0002) than the values obtained for ADP aggregation at 25 degrees C. Collagen-stimulated peak aggregation of platelets maintained at 37 degrees C for 4 and 6 hours was also significantly less than the corresponding values at 25 degrees C. Epinephrine-stimulated aggregation produced similar results, with peak platelet aggregation decreasing with time (50.3% +/- 2.9% at 6 hours compared with 38.5% +/- 3.7% at 2 hours at 25 degrees C) and high storage temperatures (there was less aggregation by platelets stored at 37 degrees C at each time period studied).
  
  
  CONCLUSIONS
  It is recommended that platelet-rich plasma for platelet aggregation testing be maintained at room temperature and be used between 2 and 4 hours after platelet donation.
authors: Department of Surgery, University of Missouri Health Sciences Center, Columbia 65212.
authors: W P
authors: WP
authors: Silver
authors: M P
authors: MP
authors: Keller
authors: R
authors: R
authors: Teel
authors: D
authors: D
authors: Silver
mesh_terms: D000244:Adenosine Diphosphate / Q000494:pharmacology; D000328:Adult; D000704:Analysis of Variance; D001782:Blood Donors; D001792:Blood Platelets / Q000187:drug effects / Q000502:physiology*; D001793:Blood Preservation; D003094:Collagen / Q000494:pharmacology; D004837:Epinephrine / Q000494:pharmacology; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D008875:Middle Aged; D010974:Platelet Aggregation / Q000187:drug effects; D013696:Temperature; D013997:Time Factors
chemical_list: D000244:Adenosine Diphosphate; D003094:Collagen; D004837:Epinephrine