Tissue-specific expression of a plant turgor-responsive gene with amino acid sequence homology to transport-facilitating proteins.
We report the isolation of a turgor-responsive gene of pea, trg-31, whose transcription induced within 30 min after the loss of leaf turgor. Structure of the coding region and 1.4 kb of 5' untranslated region was determined by DNA sequencing. A 3 kb promoter fragment from trg-31 was fused to a beta-glucuronidase (GUS) reporter gene in pBI101, tobacco leaf disks and mature plants analyzed for turgor-responsive induction of GUS mRNA. Significant amino acid sequence homology exists between trg-31 and putative transport proteins of bovine, Phaseolus, soybean and Escherichia coli membranes.
Guerrero
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CIBA-GEIGY Agricultural Biotechnology Research Unit, Research Triangle Park, NC 27709.
Crossland
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D000595:Amino Acid Sequence; D020346:Aquaporins; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D005786:Gene Expression Regulation; D017343:Genes, Plant / Q000235:genetics*; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010940:Plant Proteins / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
D020346:Aquaporins; D002352:Carrier Proteins; D010940:Plant Proteins; C080617:Trg-31 protein, Pisum sativum