Porphyrin synthesis by murine epidermal cells.
Incubation of murine epidermal cells with delta-aminolevulinic acid (ALA) resulted in a dose- and time-dependent accumulation of porphyrins, predominantly of protoporphyrin. Porphyrin accumulation decreased in the presence of iron, and the iron-mediated decrease was partially reversed by CaMg EDTA (1.25-10.0 mM), suggesting that there is functionally active ferrochelatase in these cells. This study suggests that these cells may be a useful model for the study of cutaneous porphyrin metabolism involving ferrochelatase activity.
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Dermatology Service, Department of Veterans Affairs Medical Center, New York, NY 10010.
Karas
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Sassa
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D000622:Aminolevulinic Acid / Q000494:pharmacology; D000818:Animals; D002470:Cell Survival; D002478:Cells, Cultured; D004492:Edetic Acid / Q000494:pharmacology; D000078404:Epidermal Cells; D004817:Epidermis / Q000378:metabolism; D005260:Female; D005294:Ferrochelatase / Q000502:physiology; D007501:Iron / Q000494:pharmacology; D007700:Kinetics; D051379:Mice; D008812:Mice, Hairless; D008954:Models, Biological; D011166:Porphyrins / Q000096:biosynthesis*; D012867:Skin / Q000166:cytology / Q000378:metabolism*
D011166:Porphyrins; D000622:Aminolevulinic Acid; D004492:Edetic Acid; D007501:Iron; D005294:Ferrochelatase