Polymerase chain reaction-based detection of hepatitis D virus genome in patients infected with human immunodeficiency virus.
The polymerase chain reaction (PCR) was used to detect hepatitis D (HD) viremia in patients infected with the human immunodeficiency virus (HIV). Nineteen (9%) of 206 such patients, unselected for liver disease or HBV infection, were found prospectively to be infected by HDV. Thirty-one anti-HIV-positive patients were studied by means of PCR, and the results were analyzed according to HDV and hepatitis B virus (HBV) serological status. HDV-PCR was positive in 5 patients. Two had detectable serum HDV antigen. Four patients had anti-HD IgM and IgG antibodies. All these patients were HBs antigen-positive, and 3 were HBV-DNA-positive. All the other patients were HDV-PCR-negative. Statistical analysis suggested more extensive liver damage and immunological impairment in HDV-PCR-positive patients. In this unselected HIV-infected population, HDV-RNA detection by PCR was restricted to HDV infected patients in whom 5/19 were positive. This test permitted direct diagnosis of HDV viremia and will be useful for monitoring HDV infection.
Dény
P
P
Service de Bactériologie-Virologie, CHU Avicenne, Bobigny, France.
Lecot
C
C
Jeantils
V
V
Ovaguimian
L
L
Krivitzky
A
A
Bréchot
C
C
D017088:AIDS-Related Opportunistic Infections / Q000175:diagnosis / Q000382:microbiology*; D001483:Base Sequence; D004279:DNA, Viral / Q000097:blood / Q000235:genetics; D016679:Genome, Viral; D015658:HIV Infections / Q000382:microbiology*; D003699:Hepatitis D / Q000150:complications* / Q000175:diagnosis / Q000382:microbiology; D003698:Hepatitis Delta Virus / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D012367:RNA, Viral / Q000097:blood / Q000235:genetics; D014766:Viremia / Q000175:diagnosis / Q000382:microbiology
D004279:DNA, Viral; D012367:RNA, Viral