title: Alteration of calcium mobilization in endothelial cells by volatile anesthetics.
abstract: Halothane and isoflurane have different effects on the peripheral vasculature. Halothane decreases blood pressure primarily by decreasing cardiac contractility, whereas isoflurane acts primarily as a peripheral vasodilator. These peripheral vascular actions may result from different effects of the anesthetics on endothelial cell function and the release of endothelium-derived vasoactive mediators. The ability of these agents at clinically relevant concentrations to alter agonist-induced calcium mobilization in single cultured bovine aortic endothelial cells was tested using the fluorescent indicator fura-2. Neither halothane (0.3, 0.5, and 2 mM) or isoflurane (0.5 and 2 mM) altered basal calcium ([Ca]i = 49 +/- 5 nM); however, the calcium transient normally elicited by 10 nM bradykinin (peak [Ca]i = 307 +/- 22 nM) was inhibited significantly by halothane but not isoflurane. Neither anesthetic altered the calcium response to ATP (10 microM). These findings suggest that anesthetics may have specific effects on receptor-mediated endothelial cell functions that could influence hemodynamics.
authors: Department of Anesthesia, University of Pennsylvania, Philadelphia 19104.
authors: A L
authors: AL
authors: Loeb
authors: D E
authors: DE
authors: Longnecker
authors: J R
authors: JR
authors: Williamson
mesh_terms: D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D001920:Bradykinin / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D002417:Cattle; D002478:Cells, Cultured; D004336:Drug Antagonism; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D006221:Halothane / Q000494:pharmacology*; D007530:Isoflurane / Q000494:pharmacology*
chemical_list: D000255:Adenosine Triphosphate; D007530:Isoflurane; D001920:Bradykinin; D002118:Calcium; D006221:Halothane