The enhancement by prostaglandin E2 of cumulus cell outgrowth in vitro.
Oocyte-cumulus cell complexes (OCCC) were aspirated from medium-sized (3-5 mm phi) porcine follicles to study quantitatively the process of forming monolayers. A monolayer index (MLI) was therefore developed. This index was evaluated by addition of graded quantities of fetal bovine serum. Furthermore, we observed that addition of prostaglandin E2 accelerated the monolayer formation (P < 0.001 at 20 hours versus 40 hours). The effect of PGE2 regarding both monolayer formation and cumulus cell expansion has been discussed.
Goverde
H J
HJ
Laboratory of Endocrinology and Reproduction, Medical Faculty, Nijmegen, The Netherlands.
D000818:Animals; D001790:Blood Physiological Phenomena; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003470:Culture Media; D015232:Dinoprostone / Q000494:pharmacology*; D005260:Female; D009865:Oocytes / Q000166:cytology / Q000187:drug effects*; D010053:Ovary / Q000166:cytology / Q000187:drug effects*; D013552:Swine
D003470:Culture Media; D015232:Dinoprostone