Sequence and evolution of cattle MHC class I cDNAs: concerted evolution has not taken place in cattle.
To explore genetic mechanisms responsible for major histocompatibility complex (MHC) class I evolution in the artiodactyls, we cloned and sequenced MHC class I cDNAs from a Bos taurus bull heterozygous for cattle MHC (BoLA) class I serological specificities w2 and w30. Four unique cDNAs were found, indicating the presence of at least two MHC class I loci. Analysis of these four cDNAs and all previously published BoLA cDNA sequences suggested that there may be three cattle MHC class I loci. Additionally, comparison of all of the BoLA class I cDNAs to MHC class I cDNAs of other artiodactyls showed that some of the BoLA class I cDNAs were more similar to certain sheep cDNAs than they were to other cattle cDNAs. These data indicate that each BoLA class I locus has evolved independently after an ancestral gene duplication event and that inter-locus segmental exchange or concerted evolution has not occurred rapidly enough to cause extensive divergence between the orthologous MHC class I loci of sheep and cattle.
Garber
T L
TL
Texas A & M University, Department of Veterinary Pathobiology, College Station 77843.
Hughes
A L
AL
Letvin
N L
NL
Templeton
J W
JW
Watkins
D I
DI
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D005075:Biological Evolution; D002417:Cattle / Q000235:genetics*; D004247:DNA / Q000235:genetics; D005805:Genes, MHC Class I; D006239:Haplotypes; D015395:Histocompatibility Antigens Class I / Q000235:genetics*; D008214:Lymphocytes / Q000502:physiology; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D010375:Pedigree; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid
D015395:Histocompatibility Antigens Class I; D009838:Oligodeoxyribonucleotides; D004247:DNA