Alteration of the product specificities of prenyltransferases by metal ions.
Effect of several kinds of metal ions on the chain-length distribution of reaction products of polyprenyl diphosphate synthases was investigated. In the presence of Co2+ or Mn2+ octaprenyl-, solanesyl- and decaprenyl diphosphate synthases gave a variety of polyprenyl products with the longest chains being shifted by one or two isoprene units longer than those of the products formed in the presence of Mg2+. Thus octaprenyl diphosphate synthase became to give solanesyl (C45) and decaprenyl (C50) diphosphates when Mg2+ is replaced with Co2+ or Mn2+. Similarly solanesyl diphosphate synthase produced C50- and C55- diphosphates in the presence of Co2+ or Mn2+, and decaprenyl diphosphate synthase gave C55- diphosphate as the longest product in the presence of Mn2+. More remarkable effects were observed with Mn2+ than with Co2+.
Ohnuma
S
S
Institute for Chemical Reaction Science, Tohoku University, Miyagi, Japan.
Koyama
T
T
Ogura
K
K
D002413:Cations, Divalent; D002855:Chromatography, Thin Layer; D004122:Dimethylallyltranstransferase / Q000737:chemistry / Q000378:metabolism*; D008670:Metals / Q000494:pharmacology*; D013379:Substrate Specificity
D002413:Cations, Divalent; D008670:Metals; D004122:Dimethylallyltranstransferase