title: Molecular characterization of an operon required for pertussis toxin secretion.
abstract: Mutants of Bordetella pertussis which are defective in secretion of pertussis toxin were isolated and characterized. The region of the B. pertussis chromosome identified by mutagenesis as playing a role in transport of pertussis toxin was sequenced. Analysis of this region revealed eight open reading frames, seven of which predict a protein exhibiting homology with one of the VirB proteins of Agrobacterium tumefaciens, which are involved in the transport of the T-DNA molecule across bacterial and plant membranes. Thus a set of accessory proteins are most likely involved in the secretion of pertussis toxin, and these proteins appear to be members of a family of proteins involved in the secretion of macromolecules from bacteria.
authors: Department of Microbiology and Immunology, Virginia Commonwealth University, Richmond 23298.
authors: A A
authors: AA
authors: Weiss
authors: F D
authors: FD
authors: Johnson
authors: D L
authors: DL
authors: Burns
mesh_terms: D016960:Agrobacterium tumefaciens / Q000235:genetics; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D001886:Bordetella pertussis / Q000235:genetics*; D002876:Chromosomes, Bacterial; D004269:DNA, Bacterial / Q000378:metabolism; D007700:Kinetics; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D016296:Mutagenesis; D016366:Open Reading Frames; D009876:Operon; D037342:Pertussis Toxin; D010641:Phenotype; D017386:Sequence Homology, Amino Acid; D037521:Virulence Factors; D010566:Virulence Factors, Bordetella / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D046911:Macromolecular Substances; C036483:T-DNA; D037521:Virulence Factors; D010566:Virulence Factors, Bordetella; D037342:Pertussis Toxin