Identification of alloantisera reacting with HLA-C blank (Cx52) using a mouse L-cell transfected with the HLA-Cw*1201 allele.
The HLA-C locus frequently has a serologically undefined "blank" (CwBL) specificity. A cDNA clone derived from the HLA-C gene with a blank specificity (Cx52) strongly associated with the most common haplotype in a Japanese population, A24-CwBL-B52-DR2-DQ6-DP9, has been recently cloned and sequenced in our laboratory and officially designated Cw*1201 as an allelic name, indicating that the inability to define the HLA-C antigen serologically in this haplotype is not due to an HLA-C antigen gene deletion or mutation, but to the absence of typing sera. In this paper, a mouse L-cell transfectant expressing this Cw*1201 gene product was constructed and employed for screening of alloantisera recognizing the HLA-C antigen with the Cw*1201 specificity. Two alloantisera against Cw*1201 were thus identified and characterized using HLA homozygous B-cell lines and local panel PBL cells, indicating that a transfectant expressing a single HLA provides an efficient screening system for collection of HLA-typing sera, especially reacting with serologically unclassifiable "blank" antigens.
Ando
H
H
Shonan Red Cross Blood Center, Astugi, Japan.
Miyata
Y
Y
Takata
H
H
Inoko
H
H
D000483:Alleles; D000818:Animals; D000937:Antigen-Antibody Reactions; D001402:B-Lymphocytes / Q000276:immunology; D002460:Cell Line; D003602:Cytotoxicity, Immunologic / Q000276:immunology; D005434:Flow Cytometry; D005805:Genes, MHC Class I / Q000235:genetics; D015236:HLA-C Antigens / Q000235:genetics* / Q000276:immunology*; D006801:Humans; D007518:Isoantibodies / Q000276:immunology* / Q000302:isolation & purification; D007739:L Cells; D051379:Mice; D014162:Transfection / Q000235:genetics*
D015236:HLA-C Antigens; D007518:Isoantibodies