Antiproliferative effect of pineal indoles on cultured tumor cell lines.
The in vitro antiproliferative action of pineal indoles on several tumor cell lines including melanoma (B16), sarcoma (S180), macrophage-like cell line (PU5), fibroblasts (3T3), and choriocarcinoma (JAr) was examined by measuring the incorporation of 3H-thymidine by the tumor cells, and, in the case of melanoma cells, by also measuring the incorporation of 3H-leucine and 3H-uridine. Uptake of crystal violet was used to assess the viability of the tumor cells. The order of inhibitory potency of the indoles was found to be methoxytryptamine > melatonin, methoxytryptophol, hydroxytryptophol, and methoxyindoleacetic acid > serotonin and hydroxyindoleacetic acid. The possibility of an adverse effect of the indoles on the viability of normal cells was also investigated by employing a primary culture of rat hepatocytes. The release of glutamate-oxaloacetate transaminase by hepatocytes was not affected by the indoles, although the release of glutamate-pyruvate transaminase was increased to a small extent and the uptake of crystal violet was slightly inhibited.
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Department of Biochemistry, Faculty of Medicine, Chinese University of Hong Kong, Shatin, N.T.
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Liu
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D016475:3T3 Cells; D000410:Alanine Transaminase / Q000378:metabolism; D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival; D002478:Cells, Cultured; D004261:DNA Replication / Q000187:drug effects; D005840:Gentian Violet / Q000378:metabolism; D007211:Indoles / Q000302:isolation & purification / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D008264:Macrophages / Q000187:drug effects*; D008297:Male; D051379:Mice; D010870:Pineal Gland / Q000378:metabolism; D051381:Rats; D011917:Rats, Inbred Lew; D014407:Tumor Cells, Cultured
D007211:Indoles; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D005840:Gentian Violet