A sequential staining technique for karyotypic analysis of interspecific somatic cell hybrids.
We describe a sequential staining technique for the karyotypic analysis of interspecific mouse x human somatic cell hybrids. Fluorescence in situ hybridization of samples, previously stained using standard trypsin/Giemsa protocols, was instrumental in the identification of human chromosomes present in hybrid lines. This procedure not only provided a simple distinction between human and mouse chromosomes, but it also allowed the visualization and monitoring of human sequences present in interspecific translocations and subchromosomal fragments.
Keherly
M J
MJ
Department of Human Biological Chemistry and Genetics, University of Texas, Medical Branch, Galveston 77555-0359.
Papaconstantinou
J
J
McCombs
J L
JL
D000818:Animals; D001399:Azure Stains; D002871:Chromosome Banding / Q000379:methods*; D005404:Fixatives; D006801:Humans; D006822:Hybrid Cells / Q000648:ultrastructure*; D017403:In Situ Hybridization / Q000379:methods*; D007621:Karyotyping / Q000379:methods*; D051379:Mice
D001399:Azure Stains; D005404:Fixatives