Detection of thyrotrophin releasing hormone in rat brain in vivo using novel antibody microprobes: effects of amphetamine.
Antibody microprobes of novel design were used to monitor thyrotrophin releasing hormone (TRH) in rat brain before and after-parenteral administration of amphetamine. Specific antibodies to TRH were bound to the outside of glass microprobes by adsorption to a surface of activated charcoal embedded in epoxylite resin. In male Wistar rats anaesthetised with chloral hydrate a series of antibody microprobes were implanted in forebrain. Amphetamine (10 mg/kg i.p.) caused a highly significant decrease in the binding of 125I-TRH to microprobes, indicating an increase in extracellular TRH localised in the lateral septum. There was also evidence of TRH release in the septo-hypothalamic nucleus. Neither saline, nor amphetamine at 2 mg/kg were able to evoke changes in the release of TRH at any sites.
Waterfall
A H
AH
Department of Physiology and Pharmacology, Medical School, Queens Medical Centre, Nottingham, UK.
Clarke
R W
RW
Bennett
G W
GW
D000661:Amphetamine / Q000494:pharmacology*; D000818:Animals; D000906:Antibodies / Q000276:immunology; D001923:Brain Chemistry / Q000187:drug effects*; D004567:Electrodes, Implanted; D008297:Male; D008839:Microelectrodes; D009043:Motor Activity / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D016622:Silver Staining; D013239:Stereotyped Behavior / Q000187:drug effects; D013973:Thyrotropin-Releasing Hormone / Q000032:analysis / Q000276:immunology / Q000378:metabolism*
D000906:Antibodies; D013973:Thyrotropin-Releasing Hormone; D000661:Amphetamine