Identification of a phosphoenolpyruvate:fructose phosphotransferase system (fructose-1-phosphate forming) in Listeria monocytogenes.
Listeria monocytogenes is a gram-positive bacterium whose carbohydrate metabolic pathways are poorly understood. We provide evidence for an inducible phosphoenolpyruvate (PEP):fructose phosphotransferase system (PTS) in this pathogen. The system consists of enzyme I, HPr, and a fructose-specific enzyme II complex which generates fructose-1-phosphate as the cytoplasmic product of the PTS-catalyzed vectorial phosphorylation reaction. Fructose-1-phosphate kinase then converts the product of the PTS reaction to fructose-1,6-bisphosphate. HPr was shown to be phosphorylated by [32P]PEP and enzyme I as well as by [32P]ATP and a fructose-1,6-bisphosphate-activated HPr kinase like those found in other gram-positive bacteria. Enzyme I, HPr, and the enzyme II complex of the Listeria PTS exhibit enzymatic cross-reactivity with PTS enzyme constituents from Bacillus subtilis and Staphylococcus aureus.
Mitchell
W J
WJ
Department of Biology, University of California, San Diego, La Jolla 92093-0116.
Reizer
J
J
Herring
C
C
Hoischen
C
C
Saier
M H
MH
D001426:Bacterial Proteins / Q000378:metabolism*; D004790:Enzyme Induction; D005631:Fructokinases / Q000378:metabolism; D005632:Fructose / Q000378:metabolism; D005636:Fructosephosphates / Q000378:metabolism; D008089:Listeria monocytogenes / Q000201:enzymology*; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000378:metabolism*; D010766:Phosphorylation; D017852:Phosphotransferases (Nitrogenous Group Acceptor)
D001426:Bacterial Proteins; D005636:Fructosephosphates; C032284:fructose-1-phosphate; D005632:Fructose; D005631:Fructokinases; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System; C030182:phosphocarrier protein HPr; D017852:Phosphotransferases (Nitrogenous Group Acceptor); C022360:phosphoenolpyruvate-protein phosphotransferase