Multiple column peptide synthesis, Part 2 (1, 2).
A manually operated apparatus for parallel multiple column soli-phase peptide synthesis is described. It employs Fmoc-amino acid-O-Dhbt or -Pfp esters in the continuous flow version of the polyamide method on small packed columns of kieselguhr supported resin in a reaction block of Teflon. The solvents and deprotecting reagents are dispensed from two washers in a parallel fashion and reagent consumption is low. Activated and protected amino acids are transferred from a dispenser tray as solutions, eight at a time. The use of the method is demonstrated by the synthesis of overlapping peptides from a protein structure and of analogous protease substrates. The products have been characterized by HPLC, FAB mass spectroscopy and amino acid analysis.
Meldal
M
M
Department of Chemistry, Carlsberg Laboratory, Copenhagen, Denmark.
Holm
C B
CB
Bojesen
G
G
Jakobsen
M H
MH
Holm
A
A
D000595:Amino Acid Sequence; D000942:Antigens, Bacterial / Q000737:chemistry; D001671:Biochemistry / Q000295:instrumentation*; D002851:Chromatography, High Pressure Liquid; D010450:Endopeptidases; D005069:Evaluation Studies as Topic; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000138:chemical synthesis* / Q000737:chemistry / Q000276:immunology; D012116:Resins, Plant; D016339:Spectrometry, Mass, Fast Atom Bombardment; D010566:Virulence Factors, Bordetella / Q000138:chemical synthesis / Q000737:chemistry / Q000276:immunology
D000942:Antigens, Bacterial; D009842:Oligopeptides; D012116:Resins, Plant; D010566:Virulence Factors, Bordetella; D010450:Endopeptidases