HIV-1 infection and modulation of cytokine and growth factor expression in Kaposi's sarcoma-derived cells in vitro.
OBJECTIVE
HIV-1 transcripts have been detected in AIDS-related Kaposi's sarcoma (KS) tissues within the factor XIIIa + dermal dendrocytes present in the tumor. Various cytokines and growth factors have been shown to influence the growth of KS-derived cells in vitro. HIV-1 preferentially infects CD4+ cells and has also been found to infect some CD4- cells in vitro. The susceptibility of cultured KS cells in vitro to infection with HIV-1 and the expression of interleukin (IL)-1 beta, IL-6 and basic fibroblast growth factor (bFGF) after exposure to HIV-1 was examined.


METHODS
The susceptibility of two different KS-derived cell cultures to HIV-1 infection was examined by the expression of p24 antigen, detection of proviral sequence and electron microscopy. The expression of IL-1 beta, IL-6 and bFGF was detected by enzyme-linked immunosorbent assay and reverse transcriptase polymerase chain reaction.


RESULTS
KS-derived cells can be infected by HIV-1 in vitro. Both KS-derived cells were found to express CD4 mRNA. The expression of IL-1 beta and IL-6 was increased, whereas the expression of bFGF was not stimulated after exposure of KS cells to HIV-1.


CONCLUSIONS
These experiments describe the in vitro infection of KS-derived cells by HIV-1 and the expression of various cytokines and growth factor following infection. The increased production of cytokines observed following such infection may be involved in the pathogenesis of AIDS-related KS.
Huang
Y Q
YQ
Department of Microbiology, New York University Medical Center, NY 10016.
Li
J J
JJ
Kim
K S
KS
Nicolaides
A
A
Zhang
W G
WG
Le
J
J
Poiesz
B J
BJ
Friedman-Kien
A E
AE
D000163:Acquired Immunodeficiency Syndrome / Q000150:complications; D001483:Base Sequence; D015704:CD4 Antigens / Q000096:biosynthesis*; D016222:Fibroblast Growth Factor 2 / Q000096:biosynthesis; D015972:Gene Expression Regulation, Neoplastic; D016655:HIV Core Protein p24 / Q000096:biosynthesis; D015497:HIV-1 / Q000302:isolation & purification* / Q000502:physiology; D006801:Humans; D007375:Interleukin-1 / Q000096:biosynthesis*; D015850:Interleukin-6 / Q000096:biosynthesis*; D008175:Lung Neoplasms / Q000209:etiology / Q000382:microbiology / Q000473:pathology; D008969:Molecular Sequence Data; D010996:Pleural Effusion / Q000209:etiology / Q000382:microbiology / Q000473:pathology*; D012514:Sarcoma, Kaposi / Q000209:etiology / Q000382:microbiology / Q000473:pathology*; D012878:Skin Neoplasms / Q000209:etiology / Q000382:microbiology / Q000473:pathology*; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000378:metabolism / Q000382:microbiology; D014771:Virion / Q000648:ultrastructure; D014779:Virus Replication
D015704:CD4 Antigens; D016655:HIV Core Protein p24; D007375:Interleukin-1; D015850:Interleukin-6; D016222:Fibroblast Growth Factor 2