title: Humoral immune response of humans to lipooligosaccharide and outer membrane proteins of Haemophilus ducreyi.
abstract: The humoral immune response to purified lipooligosaccharide (LOS) and outer membrane proteins (OMP) of Haemophilus ducreyi was evaluated. Sera from chancroid-endemic (Uganda, Kenya) and -nonendemic (Canada) countries were tested by an ELISA. The response to OMPs was cross-reactive with other Haemophilus species, and elevated levels of antibody were detected in patients that did not have chancroid. The LOS component stimulated an H. ducreyi-specific immune response that was detected only in patients with chancroid. The sensitivity of the LOS ELISA was 96% (95% confidence interval, 89.9%-100%) and the specificity was 97% (95% confidence interval, 95.8%-98.2%). Thus, the anti-H. ducreyi LOS immune response is a significant diagnostic and epidemiologic indicator.
authors: Department of Medical Microbiology and Infectious Diseases, University of Manitoba, Winnipeg, Canada.
authors: M J
authors: MJ
authors: Alfa
authors: N
authors: N
authors: Olson
authors: P
authors: P
authors: Degagne
authors: F
authors: F
authors: Plummer
authors: W
authors: W
authors: Namaara
authors: I
authors: I
authors: Maclean
authors: A R
authors: AR
authors: Ronald
mesh_terms: D000293:Adolescent; D000328:Adult; D000907:Antibodies, Bacterial / Q000276:immunology*; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D002602:Chancroid / Q000276:immunology*; D002648:Child; D002675:Child, Preschool; D005260:Female; D006191:Haemophilus ducreyi / Q000276:immunology*; D006801:Humans; D007223:Infant; D008070:Lipopolysaccharides / Q000276:immunology*; D008297:Male
chemical_list: D000907:Antibodies, Bacterial; D001425:Bacterial Outer Membrane Proteins; D008070:Lipopolysaccharides