title: Follicle epithelial M cells are a source of interleukin-1 in Peyer's patches.
abstract: The production of interleukin-1 (IL-1) by rabbit Peyer's patch M cells populating the follicle-associated epithelium (FAE) was studied. Sorted 5D9+ phagocytic epithelial M cells synthesized IL-1 after stimulation with lipopolysaccharide (LPS) in vitro, as evidenced by the ability of culture supernatants to induce the proliferation of the T-cell line D10.G4.1. Fixed LPS-stimulated M cells were less effective at mediating T-cell proliferation than supernatants from LPS-activated M cells. The magnitude of T-cell proliferation was M-cell concentration dependent, and proportional to the dose of LPS. The M-cell-mediated D10.G4.1 cell proliferation was inhibited > 75% with anti-IL-1 alpha, but < 50% with similar concentrations of anti-IL-1 beta. The results show that M cells secrete IL-1, and suggest the participation of M cells in the delivery of a localized co-stimulatory signal for T-cell and B-cell proliferation in the microenvironment of gut-associated lymphoid tissue (GALT).
authors: Cell Biology and Aging Section, VA Medical Center, San Francisco, California.
authors: J
authors: J
authors: Pappo
authors: R T
authors: RT
authors: Mahlman
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004848:Epithelium / Q000276:immunology; D007375:Interleukin-1 / Q000096:biosynthesis*; D008070:Lipopolysaccharides / Q000276:immunology; D010581:Peyer's Patches / Q000276:immunology*; D010586:Phagocytes / Q000276:immunology*; D011817:Rabbits
chemical_list: D007375:Interleukin-1; D008070:Lipopolysaccharides