Fatty acid-binding protein in bovine longissimus dorsi muscle.
Experiments were conducted to purify and obtain amino acid sequence data for fatty acid-binding protein from bovine longissimus dorsi muscle. 1. Separation of sarcoplasmic proteins by fractionation on Sephadex and DEAE cellulose columns indicated that a low abundance fatty acid-binding protein exists in bovine l. dorsi muscle. 2. Fatty acid-binding protein in bovine l. dorsi muscle cannot be separated from myoglobin by standard protein purification procedures. However, partial sequencing of a partially purified protein fraction indicated the presence of an NH2-terminally blocked protein. 3. Myoglobin bound palmitate under the in vitro conditions of these experiments.
Moore
K K
KK
Department of Animal Science, Texas A&M University, College Station 77843.
Cameron
P J
PJ
Ekeren
P A
PA
Smith
S B
SB
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002352:Carrier Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D002417:Cattle; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D050556:Fatty Acid-Binding Proteins; D005227:Fatty Acids / Q000378:metabolism*; D005260:Female; D008297:Male; D008969:Molecular Sequence Data; D009132:Muscles / Q000378:metabolism*; D009363:Neoplasm Proteins; D017386:Sequence Homology, Amino Acid
D000596:Amino Acids; D002352:Carrier Proteins; D050556:Fatty Acid-Binding Proteins; D005227:Fatty Acids; D009363:Neoplasm Proteins