Islet amyloid polypeptide-like immunoreactivity in human tissue and endocrine tumors.
We have detected islet amyloid polypeptide (IAPP)-like immunoreactivity (-LI) in human pancreas and in a range of endocrine tumors, including oat cell carcinoma of the lung and pancreatic tumours producing insulin, gastrin, glucagon, and vasoactive intestinal peptide. Gel permeation chromatography of the IAPP-LI revealed that, except in the carcinoid, more than 80% coeluted with synthetic human IAPP. The remaining immunoreactivity consisted of variable amounts of larger and smaller molecular forms. The concentration of IAPP-LI in the circulation of patients with diagnosed pancreatic endocrine tumors was not significantly elevated above normal fasting levels. IAPP is, therefore, produced by a range of endocrine tumors and may relate to the deposition of endocrine amyloid.
Bretherton-Watt
D
D
Department of Medicine, Royal Postgraduate Medical School, London, United Kingdom.
Ghatei
M A
MA
Bloom
S E
SE
Williams
S
S
Bloom
S R
SR
D000328:Adult; D000368:Aged; D000682:Amyloid / Q000097:blood / Q000378:metabolism*; D002850:Chromatography, Gel; D004701:Endocrine Gland Neoplasms / Q000097:blood / Q000378:metabolism*; D015408:Gastrinoma / Q000097:blood / Q000378:metabolism*; D006801:Humans; D007340:Insulinoma / Q000097:blood / Q000378:metabolism*; D058228:Islet Amyloid Polypeptide; D008875:Middle Aged; D010179:Pancreas / Q000378:metabolism*; D011863:Radioimmunoassay
D000682:Amyloid; D058228:Islet Amyloid Polypeptide