Radiation-enhanced expression of major histocompatibility complex class I antigen H-2Db in B16 melanoma cells.
Exposure of eukaryotic cells to ionizing radiation induces several cellular responses including DNA repair, arrest of DNA synthesis, and increased synthesis of specific cellular proteins. We derived from the murine melanoma cell line B16-F10 a clonal isolate (M1) that was exposed to a total dose of 5000 cGy in 25 fractions, according to a protocol that reflects the standard for current radiotherapeutic regimens. We measured, by flow cytometry of fluorescence-stained cells, the surface expression of the two major histocompatibility complex class I antigens H-2Db and H-2Kb in irradiated M1 cells and untreated M1 controls. We found that after 2000 cGy, expression of H-2Db antigen was enhanced in irradiated cells versus controls. Radiation-induced expression of H-2Db antigen appeared to be selective, since no up-regulation of the H-2Kb antigen was detectable, and persisted for at least 5 weeks following the last irradiation. Enhanced H-2Db antigen expression correlated with increased steady-state levels of H-2Db mRNA in irradiated cells. These results are consistent with the notion that enhanced expression of major histocompatibility complex class I antigens is part of a long-lasting stress response elicited in cells by radiation.
Hauser
S H
SH
Department of Radiation Oncology, New England Medical Center, Boston, Massachusetts 02111.
Calorini
L
L
Wazer
D E
DE
Gattoni-Celli
S
S
D000818:Animals; D016259:Genes, myc; D006183:H-2 Antigens / Q000096:biosynthesis / Q000235:genetics / Q000528:radiation effects*; D064532:Histocompatibility Antigen H-2D; D008546:Melanoma, Experimental / Q000276:immunology* / Q000532:radiotherapy; D051379:Mice; D012333:RNA, Messenger / Q000032:analysis; D014407:Tumor Cells, Cultured / Q000528:radiation effects; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D006183:H-2 Antigens; C032876:H-2Kb protein, mouse; D064532:Histocompatibility Antigen H-2D; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha