title: Radioimmunoimaging of xenograft pancreatic cancer with 131I-monoclonal antibody P2.
abstract: Monoclonal antibodies (McAbs) to pancreatic cancer were developed by fusing SP2/0 cells and splenocytes from Balb/c mice immunized with CH-2 cells. The specific binding rates of McAb P1 and P2 were 40.1 and 43.8%, respectively, shown by binding radioreactivity assay in vitro, which were in sharp contrast with those of control groups (p < 0.05). The biodistribution of radioiodinated McAb P2 was studied by measuring parameters of tumor-specific radioreactivity in nude mice bearing CH-2 tumors. The ratios of tumors to nontumors were all > 2 at 48 h. The localization index of cancer and the ratio of tumor to pancreas were 4.05 and 4.16, respectively, at 72 h. Therefore, 131I-McAbs may be useful for radioimmunoimaging (RII) of pancreatic cancer. After intraperitoneal injection of 131I-McAb P2 into tumor-bearing nude mice, imaging of xenograft pancreatic cancer became increasingly distinct with the nonspecific background fading, especially in the period of 72-96 h. Examination of pancreatic cancer tissues by immunohistochemical methods revealed that McAb P2 was strongly positive (86%) in comparison with other tumors and normal tissues. The results demonstrated that clinical RII of pancreatic cancer was feasible with McAb P2.
authors: Department of Surgery, University of Texas Medical Branch, Galveston 77550.
authors: C Z
authors: CZ
authors: Yao
authors: G J
authors: GJ
authors: Poston
authors: J
authors: J
authors: Ishizuka
authors: C M
authors: CM
authors: Townsend
authors: J C
authors: JC
authors: Thompson
mesh_terms: D000818:Animals; D006801:Humans; D007150:Immunohistochemistry; D007457:Iodine Radioisotopes; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D010190:Pancreatic Neoplasms / Q000000981:diagnostic imaging*; D016719:Radioimmunodetection; D014018:Tissue Distribution; D014183:Transplantation, Heterologous
chemical_list: D007457:Iodine Radioisotopes