title: Localization of neuropeptide Y and atrial natriuretic peptide in the endothelial cells of human umbilical blood vessels.
abstract: The localization of neuropeptide Y (NPY) and atrial natriuretic peptide (ANP) in the endothelial cells of human umbilical blood vessels was studied using the pre-embedding peroxidase-antiperoxidase (PAP) technique for electron microscopy and avidin-biotin-complex (ABC) immunostaining for endothelial cells cultured from umbilical vein. Subpopulations of NPY- and ANP-immunoreactive endothelial cells were present in term umbilical vein and artery. The umbilical vein contained more positive cells than the artery. The percentage of NPY- and ANP-immunoreactive umbilical vein cells in culture was 32% and 44%, respectively, out of a total of 3013 cells examined. The possibility that these potent vasoactive substances located in the endothelial cells of the non-innervated umbilical vessels are involved in the local regulation of blood flow is discussed.
authors: Department of Anatomy and Developmental Biology, University College London, United Kingdom.
authors: W Q
authors: WQ
authors: Cai
authors: P
authors: P
authors: Bodin
authors: A
authors: A
authors: Sexton
authors: A
authors: A
authors: Loesch
authors: G
authors: G
authors: Burnstock
mesh_terms: D009320:Atrial Natriuretic Factor / Q000032:analysis*; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000737:chemistry* / Q000166:cytology*; D006801:Humans; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D009478:Neuropeptide Y / Q000032:analysis*; D014469:Umbilical Arteries / Q000737:chemistry* / Q000166:cytology; D014471:Umbilical Veins / Q000737:chemistry* / Q000166:cytology
chemical_list: D009478:Neuropeptide Y; D009320:Atrial Natriuretic Factor