title: [Drosophila beta-heterochromatin: molecular organization and function. Cloning and molecular biological analysis of the lambda 20 DNA fragment from Drosophila melanogaster beta-heterochromatin].
abstract: To isolate the DNA sequences specific for the pericentric heterochromatin of Drosophila we used two CREST-autoimmune sera which bind in the Western-blot analysis the nuclear antigens of 30 kDa, 43 kDa and 45 kDa molecular weight. Cloning of the DNA fragments associated with these CREST-specific proteins of Drosophila resulted in obtaining 8 clones. One of them, lambda 20, hybridized mainly to the chromomcenter of polytene chromosomes. The further analysis indicated that the lambda 20 DNA might belong to the proximal beta-heterochromatin of the polytene chromosomes of D. melanogaster.
authors: S I
authors: SI
authors: Baĭborodin
authors: E M
authors: EM
authors: Baricheva
authors: S S
authors: SS
authors: Bogachev
authors: I V
authors: IV
authors: Borisevich
authors: O V
authors: OV
authors: Strots
authors: M A
authors: MA
authors: Filippova
authors: I V
authors: IV
authors: Sharakhov
authors: A G
authors: AG
authors: Shilov
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology; D002875:Chromosomes; D003001:Cloning, Molecular; D003429:Cross Reactions; D004247:DNA / Q000737:chemistry / Q000235:genetics*; D004331:Drosophila melanogaster / Q000235:genetics*; D006570:Heterochromatin; D006801:Humans; D007106:Immune Sera; D017403:In Situ Hybridization; D008854:Microscopy, Electron; D008938:Mitosis; D009687:Nuclear Proteins / Q000276:immunology / Q000648:ultrastructure
chemical_list: D000906:Antibodies; D006570:Heterochromatin; D007106:Immune Sera; D009687:Nuclear Proteins; D004247:DNA
vernacular_title: beta-Geterokhromatin Drosophila: molekuliarnaia organizatsiia i funktsionirovanie. Klonirovanie i molekuliarno-biologicheskiĭ analiz lamda 20 fragmenta DNK iz beta-geterokhromatina Drosophila melanogaster.