Determination of serum oxalate using peroxyoxalate chemiluminescence of free oxalic acid.
We describe a new sensitive and specific method for determination of oxalate in human serum. By using the chemiluminescence decay of monoperoxyoxalic acid very low concentrations of oxalate (200 nmol/L) can be determined. The mean serum oxalate level in apparently healthy controls was 14.5 +/- 8.5 mumol/L. Supplementation of ascorbic acid leads to an increase in serum oxalate level. While serum oxalate concentrations of calcium oxalate stone formers (x = 16.4 +/- 9.8 mumol/L) are not significantly different from the control group, an extreme increase of serum oxalate is evident in haemodialysis patients. The serum oxalate concentration decreased during dialysis treatment from 141.4 +/- 32.1 mumol/L to 36.4 +/- 12.7 mumol/L.
Albrecht
S
S
Institute of Clinical Chemistry and Laboratory Diagnostics, Medical Academy of Dresden, FRG.
Hornak
H
H
Freidt
T
T
Böhm
W D
WD
Weis
K
K
Reinschke
A
A
D001205:Ascorbic Acid / Q000494:pharmacology; D001774:Blood Chemical Analysis / Q000379:methods* / Q000706:statistics & numerical data; D005069:Evaluation Studies as Topic; D006801:Humans; D008163:Luminescent Measurements; D010070:Oxalates / Q000097:blood*; D019815:Oxalic Acid; D012016:Reference Values; D012680:Sensitivity and Specificity
D010070:Oxalates; C044622:peroxyoxalate; D019815:Oxalic Acid; D001205:Ascorbic Acid