[Effective expression of genes for interleukin-2 and its mutant analogs in E. coli cells].
Recombinant plasmids were constructed for the efficient expression in E. coli cells of the human interleukin-2 (HIL-2) gene and two its mutant analogues obtained by of chemical-enzymic synthesis and polymerase chain reaction (deletion of 14 C-terminal amino acids and a change of the codon for Trp121 to Phe). The recombinant HIL-2 but not the mutant analogues were shown to be biologically active. Both analogues obtained were weak antagonists to HIL-2.
Seregin
S V
SV
Daniliuk
N K
NK
Siniakov
A N
AN
Kamynina
T P
TP
Il'iukova
L V
LV
Sakhno
L V
LV
D001483:Base Sequence; D004926:Escherichia coli; D015870:Gene Expression; D005813:Genes, Synthetic; D006801:Humans; D007376:Interleukin-2 / Q000031:analogs & derivatives / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation; D009841:Oligonucleotides; D010957:Plasmids; D011994:Recombinant Proteins / Q000378:metabolism
D007376:Interleukin-2; D009841:Oligonucleotides; D011994:Recombinant Proteins
Effektivnaia ékspressiia v kletkakh E. coli genov interleĭkina-2 cheloveka i ego mutantnykh analogov.