Glyoxalase activities in human tumour cell lines in vitro.
The activities of glyoxalase I (EC 4.4.1.5) and glyoxalase II (EC 3.1.2.6) were measured in 3 human non-malignant and 28 tumour cell lines. The activity of glyoxalase I was in the range 321-8751 mUnits/mg of protein. The glyoxalase I/glyoxalase II activity ratio was in the range 7-147; glyoxalase I activity was always markedly greater than glyoxalase activity. There was no significant difference between glyoxalase activities in non-malignant and tumour cell lines. The activity of glyoxalase I was within and above the range found in normal human tissues and the activity of glyoxalase II was within the range and below that found in normal human tissues.
Ayoub
F
F
Department of Chemistry and Biological Chemistry, University of Essex, Colchester, U.K.
Zaman
M
M
Thornalley
P
P
Masters
J
J
D006801:Humans; D007791:Lactoylglutathione Lyase / Q000378:metabolism*; D013869:Thiolester Hydrolases / Q000378:metabolism*; D014407:Tumor Cells, Cultured; D014571:Urologic Neoplasms / Q000201:enzymology*; D000099301:Hydroxyacylglutathione Hydrolase
D007791:Lactoylglutathione Lyase; D013869:Thiolester Hydrolases; D000099301:Hydroxyacylglutathione Hydrolase