title: Detection of antigenemia by enzyme-linked immunosorbent assay in horses with experimental Ehrlichia risticii infection.
abstract: Four horses were inoculated with Ehrlichia risticii contained in either infected murine P388 D1 cells or heparinized blood from an infected horse. All 4 horses produced serum antibody, plasma antigen, and clinical signs of the disease. An enzyme-linked immunosorbent assay was used to detect antibody in the serum and was also used in conjunction with an anti-E. risticii monoclonal antibody to detect antigenemia. These laboratory and clinical findings were correlated to determine the efficiency of the antigen detection method for discerning E. risticii infection.
authors: Department of Veterinary Science, LSU Agricultural Center, Baton Rouge.
authors: R E
authors: RE
authors: Corstvet
authors: S D
authors: SD
authors: Gaunt
authors: P A
authors: PA
authors: Karns
authors: D
authors: D
authors: Burgermeister
authors: J W
authors: JW
authors: McBride
authors: S M
authors: SM
authors: Nicholson
authors: R A
authors: RA
authors: Battistini
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral / Q000097:blood*; D000956:Antigens, Viral / Q000097:blood*; D004536:Ehrlichia / Q000254:growth & development; D016873:Ehrlichiosis / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006734:Horse Diseases; D006736:Horses; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D051379:Mice; D014407:Tumor Cells, Cultured
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M