Detection of humoral antigen and antibody by enzyme-linked immunosorbent assay in horses with experimentally induced Ehrlichia equi infection.
An enzyme-linked immunosorbent assay (ELISA) was used to detect antigen in plasma and antibody in serum of 3 horses inoculated with Ehrlichia equi. Clinical signs, including rectal temperature, were correlated with the antigen and antibody detection. ELISA was very efficient in detection of serum antibody. Antigen detection using monoclonal antibodies to E. equi and ELISA should be considered as a diagnostic method.
Corstvet
R E
RE
Department of Veterinary Science, LSU Agricultural Center, Baton Rouge.
Gaunt
S D
SD
Karns
P A
PA
McBride
J W
JW
Battistini
R A
RA
Mauterer
L A
LA
Austin
F W
FW
D000818:Animals; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral / Q000097:blood*; D000956:Antigens, Viral / Q000097:blood*; D004536:Ehrlichia / Q000276:immunology; D016873:Ehrlichiosis / Q000097:blood / Q000175:diagnosis / Q000662:veterinary*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006734:Horse Diseases / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D006736:Horses; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M