Differential requirement for humoral factors for IL-2R expression of murine T cell subsets, Th1, Th2, and CD8Th clones.
Three types of alloreactive T helper (Th) cell clones, Th1, Th2, and CD8Th, were examined for the requirement of costimulatory factors for IL-2 receptor (IL-2R) expression and proliferation. When stimulated with allogeneic spleen adherent cells, these Th cell clones all expressed IL-2R strongly, whereas they expressed the receptor only weakly when subjected to allogeneic B cell stimulation. The addition of partially purified IL-2RIF to Th1, Th2, or CD8Th cell clones stimulated with B cell antigen-presenting cells increased the IL-2R expression of Th1 and CD8Th cell clones but not of the Th2 cell clone. Conversely, IL-1 up-regulated IL-2R expression of Th2 cell clones stimulated with B cell APC but not of Th1 and CD8Th cell clones. Neither IL-2RIF nor IL-1 induced IL-2R expression of unstimulated Th cell clones. IL-2R expressed in the presence of these costimulators contributed to IL-2-dependent proliferation of these cells. Thus, the expression of IL-2R was indicated to be regulated differently in three types of Th cell subsets through different cytokines.
Taira
S
S
Department of Allergology, Institute of Medical Science, University of Tokyo, Japan.
Kato
T
T
Yamamoto
K
K
Inoue
T
T
Nariuchi
H
H
D000818:Animals; D002999:Clone Cells; D016207:Cytokines / Q000276:immunology; D010450:Endopeptidases; D005786:Gene Expression Regulation; D006863:Hydrogen-Ion Concentration; D007375:Interleukin-1 / Q000276:immunology; D008213:Lymphocyte Activation; D008222:Lymphokines / Q000276:immunology*; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D015375:Receptors, Interleukin-2 / Q000096:biosynthesis*; D011994:Recombinant Proteins; D015398:Signal Transduction / Q000276:immunology; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*
D016207:Cytokines; D007375:Interleukin-1; D008222:Lymphokines; D015375:Receptors, Interleukin-2; D011994:Recombinant Proteins; D010450:Endopeptidases