title: The involvement of inositol phosphoglycan mediators in the modulation of steroidogenesis by insulin and insulin-like growth factor-I.
abstract: We have investigated the mechanisms by which insulin and insulin-like growth factor-I (IGF-I) regulate the synthesis of progesterone by swine ovary granulosa cells. Analysis of the cell density dependence of the effects of insulin and IGF-I showed that the induction of progesterone synthesis by these growth factors is consistent with an autocrine or paracrine model of action, which involves the insulin- and IGF-I-stimulated release of a soluble factor(s) into the culture medium. We have tested the hypothesis that this soluble factor(s) may be structurally related to inositol phosphoglycans, a class of putative second messengers of the action of insulin. Consistent with this hypothesis, we isolated an activator of pyruvate dehydrogenase (PDH) phosphatase from culture medium obtained from cells treated with insulin or IGF-I. Further analysis showed that specific antibodies raised against the inositol phosphoglycan anchor of the variant surface glycoprotein of Trypanosoma brucei blocked the activation of PDH phosphatase by the material isolated from the culture medium, suggesting a close structural relationship between this putative PDH phosphatase activator and inositol phosphaglycans. Pertussis toxin treatment, shown to inhibit the generation of inositol phosphoglycans in other systems, was found to inhibit the effects of insulin on progesterone synthesis in granulosa cells. Finally, the stimulatory effects of insulin and IGF-I on progesterone synthesis by intact granulosa cells were markedly inhibited by the addition of antiinositol phosphoglycan antibodies to the culture medium. Based on these observations, we propose that the release of inositol phosphoglycans into the extracellular medium plays an important role in the signaling mechanisms by which insulin and IGF-I regulate the synthesis of progesterone in swine ovary granulosa cells.
authors: Department of Pharmacology, University of Pittsburgh School of Medicine, Pennsylvania 15261.
authors: G
authors: G
authors: Romero
authors: J C
authors: JC
authors: Garmey
authors: J D
authors: JD
authors: Veldhuis
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology; D005260:Female; D006107:Granulosa Cells / Q000378:metabolism; D007295:Inositol Phosphates / Q000276:immunology / Q000378:metabolism*; D007328:Insulin / Q000494:pharmacology*; D007329:Insulin Antagonists / Q000494:pharmacology; D007334:Insulin-Like Growth Factor I / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D010053:Ovary / Q000166:cytology / Q000378:metabolism; D037342:Pertussis Toxin; D011134:Polysaccharides / Q000276:immunology / Q000378:metabolism*; D011374:Progesterone / Q000096:biosynthesis; D013256:Steroids / Q000096:biosynthesis*; D010566:Virulence Factors, Bordetella / Q000494:pharmacology
chemical_list: D000906:Antibodies; D007295:Inositol Phosphates; D007328:Insulin; D007329:Insulin Antagonists; D011134:Polysaccharides; D013256:Steroids; D010566:Virulence Factors, Bordetella; C028039:inositol phosphate glycan; D011374:Progesterone; D007334:Insulin-Like Growth Factor I; D037342:Pertussis Toxin