[Immunochemical analysis of Escherichia coli expression products of I3 and A2 genes of vaccinia virus strain L-IVL genome].
Genes I3 and A2 of the vaccinia virus strain L-IVP DNA were cloned into bacterial expressing vectors. The monospecific antisera to the expression products of these genes in E. coli were obtained. By means of immunochemical cross-analysis two polypeptides of equal electrophoretic mobility were found in the virion preparations in the band of the major envelope protein p35. The major of them is the product of gene I3, and the minor is encoded by gene A2.
Chikaev
N A
NA
Muravlev
A I
AI
Cheshenko
N V
NV
Gutorov
V V
VV
Malygin
E G
EG
D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli; D015870:Gene Expression; D005814:Genes, Viral; D007120:Immunochemistry; D014616:Vaccinia virus / Q000235:genetics*; D014759:Viral Envelope Proteins / Q000235:genetics / Q000378:metabolism
D014759:Viral Envelope Proteins
Immunokhimicheskiĭ analiz produktov ékspressii v Escherichia coli genov I3 i A2 genoma virusa ospovaktsiny shtamma L-IVL.