[Affinity modification of 40S ribosomal subparticles from human placenta by derivatives of oligoribonucleotides, containing an AUG codon. Ribosomal proteins, forming a codon-anticodon interaction region].
Using 2',3'-O-[4-N-(2-chloroethyl)-N-methylamino]benzylydene derivatives of AUGUn [32P]pC and 4-[N-(2-chloroethyl)-N-methylamino]benzylmethylphosphoamide derivatives of [32P]pAUGUn, the affinity labelling of human placental 40S ribosomal subunits was studied within 40S initiation complexes obtained in the presence of a ternary complex eIF-2.GTP.Met-tRNA(fMet). Analysis of the ribosomal proteins labelled by these mRNA analogues revealed that proteins S3/S3a, S6, S7, and S14/S15 play a key role in the interaction of the template with the 40S subunit in the presence of the ternary complex eIF-2.GTP.Met-tRNA(fMet). Proteins S2, S4, S5, S8, S9, and S17 are also involved in this interaction.
Mindus
D A
DA
Bulygin
K N
KN
Ven'iaminova
A G
AG
Vladimirov
S N
SN
Vratsikikh
L V
LV
Repkova
M N
MN
Iamkovoĭ
V I
VI
Karpova
G G
GG
D000345:Affinity Labels; D003062:Codon; D005260:Female; D006801:Humans; D009843:Oligoribonucleotides / Q000737:chemistry*; D010920:Placenta / Q000648:ultrastructure*; D011247:Pregnancy; D012358:RNA, Transfer, Met / Q000378:metabolism; D012269:Ribosomal Proteins / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism*
D000345:Affinity Labels; D003062:Codon; D009843:Oligoribonucleotides; D012358:RNA, Transfer, Met; D012269:Ribosomal Proteins
Affinnaia modifikatsiia 40S ribosomnykh subchastits iz platsenty cheloveka proizvodnymi oligoribonukleotidov, soderzhashchikh kodon AUG. Ribosomnye belki, formiruiushchie oblast' kodon-antikodonovykh vzaimodeĭstviĭ.