Oligoclonal development of germinal centre cells as detected by anti-idiotype antibodies.
Experimental models using immunization with known haptens have shown that germinal centres develop from only a limited number of precursor cells which expand in an oligoclonal fashion. Using a panel of antibodies raised against the idiotype of follicle centre cell lymphomas and known to be reactive only with a few normal B cells, we could demonstrate that individual clones can be identified within germinal centres of individual follicles of human lymph nodes and tonsils. Therefore also in humans in a non-experimental, natural situation, germinal centres expand in an oligoclonal fashion.
De Jong
D
D
Laboratory of Pathology, University of Leiden, The Netherlands.
Cramer
G
G
Brand
A
A
Kluin
P M
PM
D000888:Antibodies, Anti-Idiotypic; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology; D002675:Child, Preschool; D006801:Humans; D007124:Immunoenzyme Techniques; D007130:Immunoglobulin Idiotypes / Q000096:biosynthesis; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology; D008213:Lymphocyte Activation / Q000276:immunology; D008221:Lymphoid Tissue / Q000166:cytology* / Q000276:immunology; D008224:Lymphoma, Follicular / Q000276:immunology; D014066:Palatine Tonsil / Q000166:cytology / Q000276:immunology; D013154:Spleen / Q000166:cytology / Q000276:immunology
D000888:Antibodies, Anti-Idiotypic; D007130:Immunoglobulin Idiotypes