title: Further characterization of the assimilatory nitrate reductase from the yeast Candida nitratophila.
abstract: Nitrate reductase from the yeast Candida nitratophila was found to contain one molecule of cytochrome b557 and one atom of molybdenum per subunit. FAD/haem-dependent diaphorase activity (haem domain) was associated with a 40 kDa tryptic fragment of the subunit. The 50 amino-terminal residues of this fragment were determined, and the sequence did not show significant similarity to deduced sequences of other nitrate reductases previously published. Increasing ionic strength in vitro had a stimulatory effect on enzymic activity via stimulation of the molybdenum-dependent terminal nitrate-reducing activity. Stimulation of activity by exogenous protein (bovine serum albumin or casein) also appeared to be an ionic effect. Stimulation of catalytic activity by phosphate was a separate effect.
authors: Biochemistry Research Group, School of Biological Sciences, University of Wales, Swansea, Singleton Park, UK.
authors: C R
authors: CR
authors: Hipkin
authors: D A
authors: DA
authors: Kau
authors: A C
authors: AC
authors: Cannons
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D002175:Candida / Q000201:enzymology* / Q000235:genetics; D006418:Heme / Q000737:chemistry; D008969:Molecular Sequence Data; D008982:Molybdenum / Q000737:chemistry; D050901:Nitrate Reductase; D009565:Nitrate Reductases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D009994:Osmolar Concentration; D010710:Phosphates / Q000494:pharmacology; D011506:Proteins / Q000494:pharmacology; D014357:Trypsin / Q000494:pharmacology
chemical_list: D000596:Amino Acids; D010710:Phosphates; D011506:Proteins; D006418:Heme; D008982:Molybdenum; D009565:Nitrate Reductases; D050901:Nitrate Reductase; D014357:Trypsin