The effect of culture medium composition on ether lipid cytotoxic activity.
The effect of a serum-free medium (TNB-100), compared to RPMI 1640 containing 10% fetal bovine serum (FBS), on the lipid composition of HL60 and K562 leukemic cells was investigated. The 10% FBS RPMI medium contained approximately three times more phospholipids (PL), about three times more protein and eight times more cholesterol (CHOL) than did the TNB-100 medium. Cells cultured in TNB-100 medium, referred to as HL60-TNB and K562-TNB cells, were significantly lower in PL and CHOL than 10% FBS RPMI cells, with about a threefold higher PL-to-CHOL ratio; however, these cells were significantly higher in protein content. Cells grown in TNB-100 were also significantly more fluid than 10% FBS RPMI cells and were more sensitive to the fluidizing action of the ether lipid 1-O-octadecyl-2-O-methyl-rac-glycero-3-phosphocholine. The 50% inhibitory dose of the drug was about 50% lower in TNB-grown cells than in 10% FBS RPMI cells.
Diomede
L
L
Istituto di Ricerche Farmacologiche Mario Negri, Milan, Italy.
Piovani
B
B
Modest
E J
EJ
Salmona
M
M
D002784:Cholesterol / Q000032:analysis; D016895:Culture Media, Serum-Free; D003603:Cytotoxins / Q000494:pharmacology*; D006801:Humans; D007938:Leukemia / Q000188:drug therapy*; D008560:Membrane Fluidity / Q000187:drug effects; D009363:Neoplasm Proteins / Q000032:analysis; D010742:Phospholipid Ethers / Q000494:pharmacology*; D010743:Phospholipids / Q000032:analysis; D014407:Tumor Cells, Cultured
C076164:3-octadecyl-2-methylglycero-1-phosphocholine; D016895:Culture Media, Serum-Free; D003603:Cytotoxins; D009363:Neoplasm Proteins; D010742:Phospholipid Ethers; D010743:Phospholipids; D002784:Cholesterol