Effect of asoka on the intracellular glutathione levels and skin tumour promotion in mice.
The bark of Saraka asoca (asoka) is commonly used to treat various diseases by the Indian system of medicine and in Sri Lanka. Further purification and chemical analysis of the active compound from the bark extract of asoka showed that (-)-epicatechin was responsible for the observed antitumour/anticarcinogenic activity. Papilloma formation in mice initiated with 7,12-dimethylbenz[a]anthracene (DMBA) and promoted using croton oil was inhibited by the topical application of 100 mg/kg body weight (b.w.) of (-)-epicatechin isolated from asoka bark extract. Oral administration of the same dose restricted the growth of s.c. injected 20 methylcholanthrene (MCA) induced soil tissue fibrosarcomas significantly in mice. Elevations of almost 2-4-fold in the intracellular reduced glutathione and related enzymes viz., glutathione reductase and glutathione S-transferase of sarcoma-180 tumour cells were noted in the presence of 1 microgram/ml of (-)-epicatechin, further highlighting its antiproliferative effect.
Varghese
C D
CD
Amala Cancer Research Center and Hospital, Trichur, Kerala, India.
Nair
S C
SC
Panikkar
B
B
Panikkar
K R
KR
D015127:9,10-Dimethyl-1,2-benzanthracene; D000818:Animals; D002392:Catechin / Q000494:pharmacology*; D003436:Croton Oil; D004354:Drug Screening Assays, Antitumor; D005354:Fibrosarcoma / Q000139:chemically induced / Q000517:prevention & control*; D005980:Glutathione Reductase / Q000032:analysis*; D005982:Glutathione Transferase / Q000032:analysis*; D008297:Male; D008748:Methylcholanthrene; D051379:Mice; D010212:Papilloma / Q000139:chemically induced / Q000517:prevention & control*; D012510:Sarcoma 180 / Q000737:chemistry*; D012878:Skin Neoplasms / Q000139:chemically induced / Q000517:prevention & control*
D008748:Methylcholanthrene; D015127:9,10-Dimethyl-1,2-benzanthracene; D003436:Croton Oil; D002392:Catechin; D005980:Glutathione Reductase; D005982:Glutathione Transferase