title: Expression of the dystrophin gene in cultured fibroblasts.
abstract: The dystrophin whose defect is responsible for Duchenne and Becker muscular dystrophies is present in muscle, brain and cerebellum. We describe here the detection of dystrophin in human cultured skin fibroblasts, L809 cells and murine 3T6 cell line. Dystrophin transcripts initiated at the muscle specific first exon can also be amplified by cDNA-PCR from various fibroblastic cells. The expression of the dystrophin gene in fibroblasts could account for some abnormalities observed in patient's fibroblast cultures.
authors: ICGM, U129, INSERM, Paris, France.
authors: J P
authors: JP
authors: Hugnot
authors: H
authors: H
authors: Gilgenkrantz
authors: P
authors: P
authors: Chafey
authors: M
authors: M
authors: Lambert
authors: E
authors: E
authors: Eveno
authors: J C
authors: JC
authors: Kaplan
authors: A
authors: A
authors: Kahn
mesh_terms: D000818:Animals; D001483:Base Sequence; D001923:Brain Chemistry; D002460:Cell Line; D002478:Cells, Cultured; D004247:DNA; D016189:Dystrophin / Q000235:genetics*; D005347:Fibroblasts / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009132:Muscles / Q000737:chemistry; D016133:Polymerase Chain Reaction; D012326:RNA Splicing; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D016189:Dystrophin; D012333:RNA, Messenger; D004247:DNA