Inhibitory effect of 8-(N,N-diethylamino)octyl-3,4,5-trimethoxybenzoate hydrochloride (TMB-8) on germination of Bacillus cereus T spores.
Effect of 8-(N,N-diethylamino)octyl-3,4,5-trimethoxybenzoate hydrochloride (TMB-8), a calcium antagonist, on germination of Bacillus cereus T spores induced by L-alanine and inosine was investigated. TMB-8 had no effect on the germination of heat-activated spores, whereas it inhibited that of nonactivated spores. The TMB-8 inhibitory effect was antagonized competitively by inosine, but not by L-alanine. Addition of Ca2+ reversed the inhibitory effect of TMB-8 in a dose-related fashion. Based on the results, a role of inosine and a site(s) for inhibitory action of TMB-8 in the process leading to the germination of nonactivated spores were discussed.
Shibata
H
H
Department of Microbial Chemistry, Faculty of Pharmaceutical Sciences, University of Tokushima, Japan.
Mimura
A
A
Mitani
H
H
Tani
I
I
Hashimoto
T
T
D000409:Alanine / Q000494:pharmacology; D001409:Bacillus cereus / Q000187:drug effects* / Q000502:physiology; D002118:Calcium / Q000378:metabolism / Q000494:pharmacology; D002121:Calcium Channel Blockers / Q000037:antagonists & inhibitors / Q000494:pharmacology; D005707:Gallic Acid / Q000031:analogs & derivatives* / Q000037:antagonists & inhibitors / Q000494:pharmacology; D006358:Hot Temperature; D007288:Inosine / Q000494:pharmacology; D013171:Spores, Bacterial / Q000187:drug effects / Q000502:physiology
D002121:Calcium Channel Blockers; C006014:8-(N,N-diethylamino)octyl-3,4,5-trimethoxybenzoate; D007288:Inosine; D005707:Gallic Acid; D000409:Alanine; D002118:Calcium