title: Characterization of cellular infiltration in choroidal melanoma.
abstract: An immunohistochemical double staining technique was used to examine the characteristics of cellular infiltration in choroidal melanoma. Seven of 16 melanomas examined demonstrated high levels of cellular infiltration, mainly with T-cells and macrophages, and little infiltration with B-cells and NK cells. The majority of T-cells were of the CD8+ type and were activated, as shown by the expression of histocompatibility antigens, HLA-DR and IL2-R. Most of the infiltrating macrophages also expressed HLA-DR antigen. We also detected malignant melanocytes expressing the HLA-DR antigen. This technique could be used to study in detail cellular infiltration in a large number of archival choroidal melanomas with known clinical history, which would enable detection of markers that correlate with the prognosis of the disease.
authors: Department of Clinical Science, Institute of Ophthalmology, London, UK.
authors: K
authors: K
authors: Tobal
authors: K
authors: K
authors: Deuble
authors: A
authors: A
authors: McCartney
authors: S
authors: S
authors: Lightman
mesh_terms: D016175:B-Lymphocyte Subsets / Q000473:pathology*; D017252:CD3 Complex / Q000032:analysis; D015704:CD4 Antigens / Q000032:analysis; D016827:CD8 Antigens / Q000032:analysis; D002830:Choroid Neoplasms / Q000276:immunology / Q000473:pathology*; D006684:HLA-DR Antigens / Q000032:analysis; D006801:Humans; D007694:Killer Cells, Natural / Q000473:pathology; D016246:Lymphocytes, Tumor-Infiltrating / Q000473:pathology*; D008545:Melanoma / Q000276:immunology / Q000473:pathology*; D015375:Receptors, Interleukin-2 / Q000032:analysis; D016176:T-Lymphocyte Subsets / Q000473:pathology*
chemical_list: D017252:CD3 Complex; D015704:CD4 Antigens; D016827:CD8 Antigens; D006684:HLA-DR Antigens; D015375:Receptors, Interleukin-2