Radioimmunodetection of colorectal cancer metastases with 131I-labeled monoclonal antibody B72.3: a pilot study to determine efficacy of detection and pharmacokinetics.
We performed radioimmunoscintigraphy (RIS) and/or pharmacokinetic (PCK) studies in 12 patients with primary or metastatic colorectal carcinoma, utilizing an intravenous administration of 1-4 mCi (1 mg) of 131I-B72.3 monoclonal antibody. Metastatic lesions were correctly identified in 4/8 patients by RIS. Two patients with small lesions (> 2 cm diameter) had a false-negative RIS scan. Two patients had a true-negative RIS scan. Optimal images were obtained at 1 week postinjection. PCK studies showed that the plasma clearance of 131I-B72.3 was biexponential with an alpha-phase half-life ranging from 0.5 to 7.1 hr and a beta-phase half-life ranging from 47.5 to 85.3 hr. Systemic and renal clearance data indicated that 131I-B72.3 was cleared very slowly and almost entirely by deiodination. This pilot study was conducted to gain an understanding of the pharmacokinetics of this radiolabeled antibody. On the basis of these data, we are now studying second-generation antibodies as part of our long-range objectives to incorporate them in early detection and treatment protocols.
Stern
H
H
Department of Surgery, Mount Sinai Hospital, Toronto, Ontario, Canada.
Reilly
R
R
Gallinger
S
S
Kirsh
J
J
DeAngelis
C
C
Papa
M
M
Hay
K
K
Polihronis
J
J
Schmoker
B
B
D000328:Adult; D000368:Aged; D000951:Antigens, Neoplasm / Q000032:analysis*; D003110:Colonic Neoplasms / Q000276:immunology / Q000378:metabolism; D005188:False Negative Reactions; D005260:Female; D006023:Glycoproteins / Q000032:analysis*; D006801:Humans; D007457:Iodine Radioisotopes; D008113:Liver Neoplasms / Q000000981:diagnostic imaging / Q000378:metabolism / Q000556:secondary; D008297:Male; D008875:Middle Aged; D009362:Neoplasm Metastasis; D010599:Pharmacokinetics; D010865:Pilot Projects; D016719:Radioimmunodetection / Q000379:methods*; D012004:Rectal Neoplasms / Q000276:immunology / Q000378:metabolism
D000951:Antigens, Neoplasm; D006023:Glycoproteins; D007457:Iodine Radioisotopes; C047052:tumor-associated antigen 72