Purification and some properties of peroxidase isozymes from pineapple stem.
The enzyme peroxidase is widely distributed among the higher plants. Isozymes of peroxidase are known to occur in a variety of tissues in a large number of plant species. In this study, peroxidase isozymes were purified from the extract of pineapple stem through successive steps of ammonium sulfate fractionation, CM-Sepharose CL-6B chromatographies and DEAE-Sepharose CL-6B chromatographies. By these steps, twelve isozymes of peroxidase were obtained. Some properties of the isozymes were studied and compared.
Sung
H Y
HY
Department of Agricultural Chemistry, National Taiwan University, Taipei, R.O.C.
Yu
R H
RH
Chang
C T
CT
D000645:Ammonium Sulfate / Q000378:metabolism; D005638:Fruit / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D007527:Isoenzymes / Q000302:isolation & purification*; D007700:Kinetics; D010544:Peroxidases / Q000302:isolation & purification* / Q000378:metabolism
D007527:Isoenzymes; D010544:Peroxidases; D000645:Ammonium Sulfate