Quantitative analysis of serum proteins separated by capillary electrophoresis.
The possibility of open tubular capillary electrophoresis for clinical diagnostic use is examined. Capillary electrophoresis was performed in an untreated 50 microns (i.d.) x 100 cm (65 cm to detector) capillary with detection of absorbance at 200 nm. Conditions for the separation of serum proteins without adsorption to the capillary surface were established. Quantitative analyses of serum samples from 38 patients with liver cirrhosis, nephrotic syndrome, or polyclonal gammopathy by capillary electrophoresis were done and the results were compared with those by conventional agarose gel electrophoresis and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. All samples were analyzed in duplicate. We evaluated linearity of response, within-run CV, and the correlation between capillary electrophoresis and agarose gel electrophoresis.
Kim
J W
JW
Department of Clinical Pathology, Chungnam National University Hospital, Daejonsi, Korea.
Park
J H
JH
Park
J W
JW
Doh
H J
HJ
Heo
G S
GS
Lee
K J
KJ
D001798:Blood Proteins / Q000032:analysis*; D002197:Capillary Action; D004586:Electrophoresis / Q000379:methods*; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D006942:Hypergammaglobulinemia / Q000097:blood; D008103:Liver Cirrhosis / Q000097:blood; D009404:Nephrotic Syndrome / Q000097:blood
D001798:Blood Proteins