Structure of the gene encoding rat neutrophil chemo-attractant Gro.
A cloned rat gro gene encoding the neutrophil chemo-attractant Gro was isolated from a lambda Charon4A rat genomic library, and the nucleotide (nt) sequence of a 2500-bp fragment encompassing the coding region and 3'- and 5'-flanking regions was determined. The gene consisted of four exons separated by three introns. The transcription start point was determined by primer-extension analysis and found to be a G located 72 nt upstream from the start codon. The 5'-flanking region of the gene contained a 'TATA'-like structure and an NF-kappa B-binding sequence.
Konishi
K
K
Department of Biochemistry, Toyama Medical and Pharmaceutical University Faculty of Medicine, Japan.
Takata
Y
Y
Yamamoto
M
M
Yomogida
K
K
Watanabe
K
K
Tsurufuji
S
S
Fujioka
M
M
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D054360:Chemokine CXCL1; D019743:Chemokines, CXC; D002630:Chemotactic Factors / Q000235:genetics*; D004247:DNA; D005091:Exons; D006133:Growth Substances / Q000235:genetics*; D036341:Intercellular Signaling Peptides and Proteins; D007438:Introns; D008969:Molecular Sequence Data; D009504:Neutrophils / Q000378:metabolism*; D051381:Rats; D014158:Transcription, Genetic
D054360:Chemokine CXCL1; D019743:Chemokines, CXC; D002630:Chemotactic Factors; C513297:Cxcl1 protein, rat; D006133:Growth Substances; D036341:Intercellular Signaling Peptides and Proteins; D004247:DNA