Development and validation of a solvent extraction technique for determination of Cu-PTSM in blood.
The partitioning of [67Cu]Cu-PTSM between plasma and red blood cells (RBC) was investigated in vitro with human, rat, pig and dog blood. Significant inter-species variability is observed in the plasma/RBC partitioning of tracer, ranging from c. 75% association with plasma in human blood to only c. 35% association with plasma in dog blood. This inter-species difference results from selective association of the [67Cu]Cu-PTSM tracer with human albumin. When [67Cu]Cu-PTSM is mixed with human blood in vitro at 37 degrees C the fraction of 67Cu-radioactivity that remains plasma-associated decreases with time, apparently due to the expected intracellular decomposition of the Cu-PTSM complex by RBC; however, this process is sufficiently slow that it should have limited influence on [62Cu]CU-PTSM biodistribution following intravenous injection. Octanol extraction of blood was found to be an effective technique for quantitating the amount of intact [67Cu]Cu-PTSM complex in blood samples. When imaging with [62Cu]Cu-PTSM, octanol extraction may be useful for determining the [62Cu]Cu-PTSM content of arterial blood samples to establish a true radiotracer input function.
Mathias
C J
CJ
Department of Medicinal Chemistry, School of Pharmacy, Purdue University, West Lafayette, IN 47907-1333.
Bergmann
S R
SR
Green
M A
MA
D000818:Animals; D003301:Copper Radioisotopes; D004285:Dogs; D004912:Erythrocytes / Q000378:metabolism; D006801:Humans; D008297:Male; D009942:Organometallic Compounds / Q000097:blood* / Q000493:pharmacokinetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D012997:Solvents; D013552:Swine; D013882:Thiosemicarbazones / Q000097:blood* / Q000493:pharmacokinetics; D014018:Tissue Distribution
D003301:Copper Radioisotopes; D009942:Organometallic Compounds; D012997:Solvents; D013882:Thiosemicarbazones; C063293:copper (II) pyruvaldehyde bis(N(4)-dimethylthiosemicarbazone)