title: Acute effects of administration of porcine growth hormone on circulating levels of hormones and metabolites in 20-, 40-, and 60-kilogram gilts.
abstract: Three groups of eight gilts weighing 20, 40, or 60 kg were fitted with indwelling venous catheters to determine daily integrated circulating levels (DICL, serum concentration above baseline x time) of insulin, growth hormone (GH), glucagon, glucose, urea, and nonesterified fatty acids (NEFA) in response to acute challenge with porcine pituitary GH (pGH). Pigs were fed a common diet containing 18% CP and 3.5 Mcal of DE/kg between 0800 and 1200 (85% of ad libitum). Blood and urine were collected at 2- or 4-h intervals for 4 d. On d 2, 3, and 4, four pigs in each group were injected i.m. at 0800 with pGH (.1 mg/kg) and four pigs (controls) were injected with buffer. In control pigs DICL of GH was 20.4, 14.1, and 10.2 ng pGH.h.mL-1 in 20-, 40-, and 60-kg pigs, respectively. The DICL of GH in pGH-treated pigs was 4.2-, 7.0-, and 10.7-fold greater in 20-, 40-, and 60-kg pigs, respectively, than in controls. The DICL of insulin in control pigs was 7.9, 9.7, and 9.4 ng.h.mL-1 and was increased (P < .001) in pGH-treated pigs by 118, 213, and 276% in 20-, 40-, and 60-kg pigs, respectively. Although serum levels of glucose were increased (P < .001) by pGH treatment, the acute elevation observed in 60-kg pigs was more consistent relative to 20- and 40-kg pigs. In contrast, the acute reduction in blood urea upon pGH injection was more apparent in 20-kg gilts.(ABSTRACT TRUNCATED AT 250 WORDS)
authors: Livestock and Poultry Science Institute, Beltsville Agricultural Research Center, ARS, USDA, MD 20705.
authors: T J
authors: TJ
authors: Caperna
authors: N C
authors: NC
authors: Steele
authors: C M
authors: CM
authors: Evock-Clover
authors: D
authors: D
authors: Brocht
authors: J P
authors: JP
authors: McMurtry
authors: R W
authors: RW
authors: Rosebrough
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D001835:Body Weight; D003404:Creatinine / Q000652:urine; D004435:Eating; D005230:Fatty Acids, Nonesterified / Q000097:blood; D005260:Female; D005934:Glucagon / Q000097:blood; D013006:Growth Hormone / Q000097:blood / Q000494:pharmacology*; D007328:Insulin / Q000097:blood; D013552:Swine / Q000097:blood*; D014508:Urea / Q000097:blood / Q000652:urine
chemical_list: D001786:Blood Glucose; D005230:Fatty Acids, Nonesterified; D007328:Insulin; D014508:Urea; D013006:Growth Hormone; D005934:Glucagon; D003404:Creatinine