Cell kinetic analysis after irradiation in L929- and LLC-MK2-cells by a BrdU/DNA assay.
Exponentially growing L929- and LLC-MK2-cells were X-irradiated in vitro. Irradiation-induced cell cycle kinetic effects were examined by the calculation of the cell number doubling time (TD), the duration of the cell cycle phases by the BrdU/DNA assay and the number of micronuclei. The number of cells arrested in G2/M and the duration of the delay are dose-related. The effect of irradiation on the duration of cell cycle phases was estimated by following the BrdU-labeled cells through the cell cycle. With increasing radiation doses the duration of the G2/M-phase increases whereas G1- and S-phase show only slight variations. Due to the problems involved in evaluation of radiation effects on the cell cycle a comparison with other methods proves the BrdU/DNA-assay to be a valuable instrument in those experiments. The micronucleus frequency is dependent on irradiation dose. However, after 7.52 Gy the number of micronuclei increases, whereas no cells in G1-phase and no increase of cell number could be detected, indicating a mechanism of micronucleus formation that is not linked with mitosis.
Gilbertz
K P
KP
Labor für Molekulare Zellbiologie und Allgemeine Pathologie, Universität Ulm, München.
Baaske
C
C
van Beuningen
D
D
D000818:Animals; D001973:Bromodeoxyuridine; D002453:Cell Cycle / Q000528:radiation effects*; D002460:Cell Line; D004247:DNA / Q000235:genetics; D007668:Kidney / Q000166:cytology; D007739:L Cells; D008253:Macaca mulatta; D051379:Mice; D015162:Micronucleus Tests
D004247:DNA; D001973:Bromodeoxyuridine