Induction of flavin-containing monooxygenase (FMO B) in rabbit lung and kidney by sex steroids and glucocorticoids.
Modulation of lung and kidney levels of flavin-containing monooxygenase (FMO) isoform B following sc administration of dexamethasone, progesterone, beta-estradiol, aldosterone, or beta-estradiol plus progesterone was examined in male rabbits. Both dexamethasone and progesterone increased FMO B protein levels in lung. N-Oxygenation of N,N'-dimethylaniline (DMA) was significantly induced in lung microsomes from rabbits treated with progesterone. In kidney, only dexamethasone was effective in inducing FMO B protein levels and DMA N-oxygenation. Surgical implantation of osmotic pumps containing beta-estradiol or progesterone into non-pregnant female rabbits resulted in elevated plasma levels of these steroids after 4-5 days at which point the lung levels of FMO B were induced by 35 and 82% for beta-estradiol and progesterone, respectively. The responsiveness of FMO B in lung to both progesterone and glucocorticoids and in kidney to only the latter is consistent with the developmental pattern of both maternal FMO B and peak plasma levels of these steroids during pregnancy.
Lee
M Y
MY
Toxicology Program, Oregon State University, Corvallis, Oregon 97331-6602.
Clark
J E
JE
Williams
D E
DE
D000450:Aldosterone / Q000494:pharmacology; D000814:Aniline Compounds / Q000378:metabolism; D000818:Animals; D003907:Dexamethasone / Q000494:pharmacology; D004790:Enzyme Induction / Q000187:drug effects; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D005938:Glucocorticoids / Q000494:pharmacology*; D007263:Infusions, Parenteral; D007668:Kidney / Q000187:drug effects / Q000201:enzymology*; D008168:Lung / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D008861:Microsomes / Q000201:enzymology; D010105:Oxygenases / Q000096:biosynthesis*; D011374:Progesterone / Q000494:pharmacology; D011817:Rabbits; D012727:Sex Characteristics; D013256:Steroids / Q000494:pharmacology*
D000814:Aniline Compounds; D005938:Glucocorticoids; D013256:Steroids; D000450:Aldosterone; D011374:Progesterone; D004958:Estradiol; C015157:N,N-dimethylaniline; D003907:Dexamethasone; D010105:Oxygenases; C020066:dimethylaniline monooxygenase (N-oxide forming)