Developmental control elements in the promoter of a Drosophila vitelline membrane gene.
DNA fragments from the 5' flanking region of Drosophila melanogaster vitelline membrane gene VM26A1 were fused to hsp/lacZ reporter genes, reintroduced via P-element-mediated germ line transformation, and functionally assayed by histological staining for beta-galactosidase activity. A developmental control element capable of promoting the basic vitelline membrane (VM) expression pattern was found within a 176-bp region. Additional multiple genetic control elements were found to be involved in modifying the level and spatial pattern of reporter gene expression but these required the 176-bp element in order to function. Unexpectedly, in isolation this 176-bp core element also promotes abnormal expression of the reporter gene in border cells of stage 8 and 9 egg chambers. This abnormal border cell expression is apparently suppressed in the native gene by an adjacent negative control element.
Jin
J
J
Department of Biology, Boston College, Chestnut Hill, Massachusetts 02167.
Petri
W H
WH
D000818:Animals; D004330:Drosophila / Q000235:genetics*; D015870:Gene Expression / Q000235:genetics; D005809:Genes, Regulator; D011401:Promoter Regions, Genetic; D014817:Vitelline Membrane; D014818:Vitellogenesis / Q000235:genetics*; D001616:beta-Galactosidase
D001616:beta-Galactosidase