Diverse T cell receptor V beta gene usage in the central nervous system in experimental allergic encephalomyelitis.
The repertoire of TCR V beta genes transcribed and expressed within the central nervous system was determined in mice with experimental allergic encephalomyelitis. Disease was induced in (PL/J x SJL/)F1 mice by immunizing with myelin basic protein-acetylated peptide 1-11, and mice were sacrificed at intervals from day 3 postimmunization to 3 wk after recovery from disease. Transcription of V beta genes was determined by reverse transcriptase polymerase chain reaction on RNA extracted from spinal cord, and expression of the V beta gene products was detected by immunohistochemistry with mAb specific for various V beta proteins. Multiple V beta genes were found to be transcribed and expressed in the central nervous system starting 7 days after immunization, and continuing up to 3 wk after clinical recovery. Preferential utilization of a single TCR V beta gene was not detected in the central nervous system at any time in the course of disease.
Bell
R B
RB
Department of Neurology, Stanford University, CA 94305.
Lindsey
J W
JW
Sobel
R A
RA
Hodgkinson
S
S
Steinman
L
L
D000818:Animals; D015703:Antigens, CD / Q000032:analysis; D001483:Base Sequence; D002490:Central Nervous System / Q000378:metabolism*; D006224:Cricetinae; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000235:genetics / Q000276:immunology*; D005260:Female; D005796:Genes; D007150:Immunohistochemistry; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D051381:Rats; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics*; D014158:Transcription, Genetic
D015703:Antigens, CD; D016693:Receptors, Antigen, T-Cell, alpha-beta