Validation of the dual analyte assay of the oestrone:pregnanediol ratio in monitoring ovarian function.
The purpose of this study was to validate the dual analyte system (Boots Celltech Ltd, Slough, UK) as a marker of ovarian function. For this purpose the urinary profile of the ratio of oestrone-3-glucuronide to pregnanediol-3-glucuronide in urine (E13G/PD3G) was compared with plasma concentrations of oestradiol, progesterone, luteinizing hormone (LH) and follicle stimulating hormone from day 8-24 of the menstrual cycle in 23 women. Daily transvaginal ultrasound was also performed from day 8 of the cycle until sonographic evidence of follicular rupture. The ratio of urinary metabolites exhibited a maximum value close to ovulation. In the majority of cases this coincided with the day of LH peak, and was followed by follicular rupture within the next 24 h in 74% of cases. The assay gives a good marker of ovulation, however, it is unlikely to help in detecting the initiation of the fertile period or the quality of the menstrual cycle.
Fabres
C
C
Unit of Reproductive Medicine, Clinica Las Condes Hospital, Santiago, Chile.
Zegers-Hochschild
F
F
Altieri
E
E
Llados
C
C
D000328:Adult; D004797:Enzyme-Linked Immunosorbent Assay; D004958:Estradiol / Q000097:blood; D004970:Estrone / Q000652:urine*; D005260:Female; D005640:Follicle Stimulating Hormone / Q000097:blood; D006728:Hormones / Q000097:blood; D006801:Humans; D007986:Luteinizing Hormone / Q000097:blood; D008991:Monitoring, Physiologic / Q000379:methods; D006080:Ovarian Follicle / Q000000981:diagnostic imaging; D010060:Ovulation / Q000502:physiology*; D011276:Pregnanediol / Q000652:urine*; D011374:Progesterone / Q000097:blood; D015203:Reproducibility of Results; D014463:Ultrasonography
D006728:Hormones; D004970:Estrone; D011374:Progesterone; D004958:Estradiol; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D011276:Pregnanediol