Binding sites for short-term glycated albumin on peritoneal cells of the rat.
The interaction of in vitro short-term glycated rat serum albumin with rat peritoneal cells (40% macrophages) was investigated. Using 125I-labeled albumins the following results were obtained. Glycated albumins showed a binding reaction at 4 degrees C, which appeared to reach equilibrium within 2 h. The concentration-dependent binding of glycated albumin showed saturation. Binding data evaluated for glycated albumin using the Sips equation are: average association constant Ko = 3.15 x 10(7) M-1 with a heterogeneity index of a = 0.8 and 1.12 x 10(4) binding sites per cell. Such binding sites were identified in 40% of the peritoneal cell preparations studied. Native albumins, maleylated albumin, chondroitinsulfates, polylysine, lysine, fructose, glucose and hexitol-lysine could not compete with radio-labeled glycated rat albumin for its binding site on peritoneal cells. Effective competitors were glycated human serum albumin, glycated polylysine and fructose-lysine. Although the contamination with minute amounts of advanced glycosylation end products (AGE) could not be excluded, short-term glycated albumin was found to be bound to membranes of peritoneal phagocytotic cells by fructose-lysine specific proteins, whose approximately defined molecular masses of 290 kDa are distinct from hitherto described binding proteins for AGE- and aldehyde-modified proteins or for the scavenger receptors.
Krantz
S
S
Institut für Biochemie, Ernst-Moritz-Arndt-Universität, Greifswald, Germany.
Brandt
R
R
Gromoll
B
B
D000818:Animals; D001665:Binding Sites; D001667:Binding, Competitive; D002462:Cell Membrane / Q000378:metabolism; D017127:Glycation End Products, Advanced; D006801:Humans; D007457:Iodine Radioisotopes; D008264:Macrophages / Q000378:metabolism*; D010529:Peritoneal Cavity; D051381:Rats; D012709:Serum Albumin / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D000093423:Glycated Serum Albumin
D017127:Glycation End Products, Advanced; D007457:Iodine Radioisotopes; D012709:Serum Albumin; D000093423:Glycated Serum Albumin