Semiquantitative immunohistochemical studies of blood group antigen A, B, H, Le(a), Le(b) structures and Ii backbone chains in the normal human cervix and in cervical adenocarcinoma.
Epithelia frequently express blood group antigens and these are often perturbed in neoplasia. This study has characterized the range of expression of ABH and Lewis terminal structures and the Ii backbone chains in the normal human cervix by semiquantitative immunohistochemistry. Effects of the secretor gene were defined by determination of salivary secretor status. Modifications of blood group antigen expression in cervical adenocarcinoma were also addressed. Normal cervical squamous and glandular epithelia showed a range of expression of the antigens studied. Lewis-gene-negative cases showed no expression of Lewis antigens. Secretor status had no effect on ABH expression in squamous epithelium, but it did have a marked effect on ABH expression in glands and on Le(b) expression in both squamous and glandular epithelia. Patterns of expression of i chains in squamous epithelium suggest that these may be the carriers of ABH and Lewis antigens in a proportion of cases. Distinct patterns of expression were seen in glandular tubal metaplasia and in endothelium. Adenocarcinomas showed topographical rather than quantitative changes in blood group antigen expression with more extensive luminal expression of ABH, Lewis and Ii structures than that seen in normal glands. This change is distinct from those usually associated with malignancy.
Griffin
N R
NR
Department of Pathology, University of Leeds, UK.
Wells
M
M
D000017:ABO Blood-Group System / Q000276:immunology; D000230:Adenocarcinoma / Q000276:immunology*; D001789:Blood Group Antigens / Q000276:immunology*; D002240:Carbohydrate Sequence; D002584:Cervix Uteri / Q000276:immunology*; D005260:Female; D006801:Humans; D007048:I Blood-Group System / Q000276:immunology; D007124:Immunoenzyme Techniques; D007519:Isoantigens / Q000032:analysis*; D007983:Lewis Blood Group Antigens / Q000276:immunology; D008969:Molecular Sequence Data; D002583:Uterine Cervical Neoplasms / Q000276:immunology*
D000017:ABO Blood-Group System; D001789:Blood Group Antigens; D007048:I Blood-Group System; D007519:Isoantigens; D007983:Lewis Blood Group Antigens