Recovery of decreased antibody production by ceruloplasmin in the macular mutant mouse.
The macular mutant mouse is a murine model of Menkes' kinky hair disease, characterized by a serum copper and ceruloplasmin deficiency. The hemizygote (ml/y) had a decreased antibody response to sheep red blood cells (SRBC). 1) In vivo; the administration of ceruloplasmin recovered antibody production in ml/y mice, but, had no effect on that of normal mice (+/y). The administration of heat treated ceruloplasmin did not increase antibody production in ml/y mice, whereas the administration of various concentrations of copper suppressed the anti-SRBC plaque-forming response in both ml/y and +/y mice. 2) in vitro; ceruloplasmin had no effect upon antibody production against SRBC in both ml/y and +/y mouse spleen cells. In contrast to ceruloplasmin, 1 microgram/ml of copper inhibited the antibody production in spleen cells from both +/y and ml/y mice as well as in vivo. These findings suggested that the decreased antibody production in ml/y mice was considered to be due the decreased ceruloplasmin.
Nakagawa
S
S
Faculty of Pharmaceutical Sciences, Kobe Gakuin University, Japan.
Fukata
Y
Y
Miyake
M
M
Hama
T
T
D000818:Animals; D000917:Antibody Formation / Q000187:drug effects* / Q000276:immunology; D002570:Ceruloplasmin / Q000172:deficiency* / Q000276:immunology; D003300:Copper / Q000097:blood / Q000276:immunology / Q000494:pharmacology*; D007275:Injections, Intravenous; D008297:Male; D051379:Mice; D008818:Mice, Neurologic Mutants; D013045:Species Specificity; D013154:Spleen / Q000187:drug effects / Q000276:immunology / Q000378:metabolism
D003300:Copper; D002570:Ceruloplasmin