title: Regulation of intestinal concentration of cholecystokinin by bile and/or pancreatic juice.
abstract: Pancreatic exocrine secretion in conscious rats is regulated by intraluminal bile and/or pancreatic juice. Exclusion of bile and/or pancreatic juice from the intestinal lumen caused cholecystokinin (CCK) release and stimulated pancreatic secretion. CCK in the plasma is mainly derived from endocrine cells in the proximal small intestinal mucosa. We examined the changes in CCK concentrations in the intestinal mucosa and compared them to those of plasma CCK concentrations and the changes of luminal trypsin activities after bile and/or pancreatic juice diversion in conscious rats. Rats with bile and pancreatic fistulae were used. Each treatment of bile, pancreatic juice, and bile-pancreatic juice diversion decreased luminal trypsin activity and increased plasma and intestinal CCK concentrations. The potency of the stimulatory effect on plasma and intestinal CCK concentrations was bile-pancreatic juice diversion > pancreatic juice diversion > or = bile diversion. Neither plasma CCK concentration nor intestinal CCK concentration was in inverse proportion to trypsin activity. The plasma CCK concentration did not parallel intestinal CCK concentration. Intravenous infusion of CCK-8 (300 pmol/kg/hr) did not increase CCK concentration in the intestinal mucosa. It was proposed that bile and/or pancreatic juice in the intestinal lumen regulated CCK concentrations not only in the plasma but also in the intestinal mucosa.
authors: Department of Clinical Physiology, Tokyo Metropolitan Institute of Gerontology, Japan.
authors: K
authors: K
authors: Miyasaka
authors: A
authors: A
authors: Funakoshi
authors: M
authors: M
authors: Matsumoto
authors: K
authors: K
authors: Kitani
mesh_terms: D000818:Animals; D001646:Bile / Q000187:drug effects / Q000502:physiology*; D002766:Cholecystokinin / Q000032:analysis / Q000187:drug effects / Q000378:metabolism*; D004386:Duodenum / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D005260:Female; D007413:Intestinal Mucosa / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D009929:Organ Size / Q000187:drug effects; D010179:Pancreas / Q000187:drug effects / Q000378:metabolism; D010189:Pancreatic Juice / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D012844:Sincalide / Q000494:pharmacology; D013997:Time Factors
chemical_list: D002766:Cholecystokinin; D012844:Sincalide