Identification of host-protective antigens of Taenia ovis oncospheres.
Sheep were fully protected against challenge infection following immunization with a homogenate of T. ovis oncospheres. Ultracentrifugation of sonicated oncospheres either alone or in the presence of a range of detergents did not reduce the immunogenicity of the extracts. Solubilization of oncosphere extracts in non-ionic detergents or sodium dodecyl sulphate (SDS) enabled analysis of host-protective antigens by isoelectric focusing (IEF) and electrophoresis in polyacrylamide gels (SDS-PAGE), respectively. Immunoblotting analysis of oncosphere antigens with immune sheep sera identified predominantly two groups of antigens with relative mobilities of 31-34 kDa and 47-52 kDa with a common isoelectric point of 5.8. The immunogenicity of these antigens was confirmed in vaccination trials using appropriate fractions cut from SDS-PAGE gels and agarose IEF gels. Affinity-purified antibodies prepared against the candidate antigens were used to select the corresponding recombinant DNA-derived polypeptides, one of which was subsequently found to be host-protective.
Harrison
G B
GB
Pitman-Moore New Zealand Limited, Research and Development Department, Upper Hutt, New Zealand.
Heath
D D
DD
Dempster
R P
RP
Lawrence
S B
SB
Lightowlers
M W
MW
Rickard
M D
MD
D000818:Animals; D000909:Antibodies, Helminth / Q000276:immunology; D000917:Antibody Formation; D000947:Antigens, Helminth / Q000276:immunology* / Q000302:isolation & purification; D007116:Immunization, Passive; D012756:Sheep; D012757:Sheep Diseases / Q000276:immunology* / Q000517:prevention & control; D013621:Taenia / Q000276:immunology*; D013622:Taeniasis / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D014611:Vaccination
D000909:Antibodies, Helminth; D000947:Antigens, Helminth