Thioredoxin from activated macrophages as a trophic factor for central cholinergic neurons in vitro.
The conditioned medium from gamma-interferon-stimulated macrophages elevated the choline acetyltransferase activity in mouse septal neurons as well as in cholinergic hybrid cell lines SN6.10.2.2 in vitro. After purification and sequencing, the active fraction was identical to thioredoxin (TRX), and the neurotrophic activity of recombinant TRX was confirmed.
Endoh
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Division of Demyelinating Disease and Aging, National Institute of Neuroscience, NCNP, Tokyo, Japan.
Kunishita
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T
Tabira
T
T
D000595:Amino Acid Sequence; D000818:Animals; D002478:Cells, Cultured; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D006822:Hybrid Cells; D007371:Interferon-gamma / Q000494:pharmacology; D008262:Macrophage Activation; D008264:Macrophages / Q000187:drug effects / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009474:Neurons / Q000166:cytology* / Q000378:metabolism; D011950:Receptors, Cholinergic / Q000378:metabolism*; D013879:Thioredoxins / Q000737:chemistry
D011950:Receptors, Cholinergic; D013879:Thioredoxins; D007371:Interferon-gamma; D002795:Choline O-Acetyltransferase