title: Cloning and sequence determination of the valS gene, encoding valyl-tRNA synthetase in Lactobacillus casei.
abstract: The DNA sequence of the valS gene from Lactobacillus casei and the predicted amino acid sequence of its valyl-tRNA synthetase product have been determined. An open reading frame coding for a protein of 901 amino acids was found. A clone containing the intact L. casei valS gene functionally complemented the temperature-sensitive growth of the valS mutant strain 236c of Escherichia coli. The valS gene and the downstream folylpolyglutamate synthetase gene are transcribed in the same direction but are separated by a putative transcription terminator.
authors: Department of Microbiology, University of Toronto, Ontario, Canada.
authors: B V
authors: BV
authors: Taylor
authors: J
authors: J
authors: Toy
authors: T L
authors: TL
authors: Sit
authors: A L
authors: AL
authors: Bognar
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D007780:Lacticaseibacillus casei / Q000201:enzymology / Q000235:genetics*; D008969:Molecular Sequence Data; D010957:Plasmids; D014637:Valine-tRNA Ligase / Q000737:chemistry / Q000235:genetics*
chemical_list: D014637:Valine-tRNA Ligase