Isolation of <Glu-Asn-Phe-His-Leu-Arg-Pro-NH2 (Antho-RPamide II), a novel, biologically active neuropeptide from sea anemones.
Using a radioimmunoassay against the C-terminal sequence Arg-Pro-NH2 (RPamide) we have isolated the neuropeptide <Glu-Asn-Phe-His-Leu-Arg-Pro-NH2 (Antho-RPamide II) from extracts of the sea anemone Anthopleura elegantissima. Addition of low concentrations of Antho-RPamide II to a tentacle preparation of sea anemones inhibited the spontaneous, rhythmic contractions, suggesting that the peptide is a neurotransmitter or neuromodulator.
Carstensen
K
K
Center for Molecular Neurobiology (ZMNH), University of Hamburg, Germany.
McFarlane
I D
ID
Rinehart
K L
KL
Hudman
D
D
Sun
F
F
Grimmelikhuijzen
C J
CJ
D000595:Amino Acid Sequence; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009068:Movement / Q000187:drug effects; D009479:Neuropeptides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D011863:Radioimmunoassay; D012615:Sea Anemones / Q000737:chemistry*
D009479:Neuropeptides; C080964:Antho-RPamide II