A new Danish Listeria monocytogenes phage typing system.
A phage typing system was developed for Listeria monocytogenes. Phages were released from clinical and from food strains of L. monocytogenes by mitomycin C induction. The system consists of two subsystems, one of which is used to type L. monocytogenes serotype 1 strains, containing 12 phages, and a second which is used to type serotype 4 strains, containing 14 other phages. For the serotype 1 subsystem, the reproducibility was > or = 90%, the typability 92%, and the discriminatory power, as judged by the discriminatory index, 80%. The corresponding figures for the serotype 4 subsystem were: reproducibility > or = 94%, typability 87%, and discriminatory index 87%. The performance of the whole typing system is sufficient for it to be used for screening purposes.
Gerner-Smidt
P
P
Department of Clinical Microbiology, Statens Seruminstitut, Copenhagen, Denmark.
Rosdahl
V T
VT
Frederiksen
W
W
D001434:Bacteriophage Typing / Q000379:methods* / Q000592:standards; D005069:Evaluation Studies as Topic; D005516:Food Microbiology; D006801:Humans; D008089:Listeria monocytogenes / Q000145:classification* / Q000187:drug effects; D008937:Mitomycins / Q000494:pharmacology
D008937:Mitomycins