title: K(+)-dependent Na+/Ca2+ exchange in human platelets.
abstract: Ca2+ influx via Na+/Ca2+ exchange was examined using fura-2 fluorescence techniques in human platelets loaded internally with Na+ by pretreatment with ouabain. In 140 mM LiCl, Ca2+ influx via the exchanger was completely dependent upon the presence of extracellular K+ (Km approximately 1 mM). In 140 mM N-methyl-D-glucamine (NMDG), Ca2+ influx was stimulated by K+ but was not absolutely dependent upon it; the inclusion of 20 mM NaCl in the NMDG medium restored the K+ dependence of Ca2+ influx. Stimulation of Ca2+ influx by K+ was confirmed by 45Ca2+ flux studies. 86Rb+ fluxes were measured to determine if Ca2+ and Rb+ were co-transported by the exchanger. The presence of extracellular Ca2+ stimulated 86Rb+ influx in ouabain-treated platelets in 160 mM LiCl or NMDG. The Rb+/Ca2+ influx ratio was 0.42 +/- 0.04 (n = 3) at [Rb+] = 0.2 mM and 0.89 at [Rb+] = 1.3 mM. Neither K(+)-dependent Ca2+ influx nor Ca(2+)-dependent Rb+ influx was observed in 160 mM NaCl or in platelets that had not been pretreated with ouabain, indicating that these fluxes resulted from Na+/Ca2+ exchange activity. Na(+)-dependent Ca2+ efflux was also shown to be dependent upon the presence of internal K+. These properties are similar to those of the Na+/Ca(2+)-K+ exchanger found in retinal rods, as distinct from the more widely distributed cardiac-type exchanger.
authors: Hypertension Research Center, New Jersey Medical School, University of Medicine and Dentistry of New Jersey, Newark 07103.
authors: M
authors: M
authors: Kimura
authors: A
authors: A
authors: Aviv
authors: J P
authors: JP
authors: Reeves
mesh_terms: D001792:Blood Platelets / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D010042:Ouabain / Q000494:pharmacology; D011188:Potassium / Q000378:metabolism*; D012964:Sodium / Q000378:metabolism*; D019831:Sodium-Calcium Exchanger
chemical_list: D002352:Carrier Proteins; D019831:Sodium-Calcium Exchanger; D010042:Ouabain; D012964:Sodium; D011188:Potassium; D002118:Calcium