Characterization of T-cell clones generated from skin of patients with psoriasis.
BACKGROUND
The infiltrating cells in psoriasis include a subpopulation of autoreactive T cells.


OBJECTIVE
The aim of this study was to further characterize skin-infiltrating T lymphocytes in patients with psoriasis.


METHODS
Forty-five T-cell clones were generated from skin biopsy specimens from two patients.


RESULTS
Phenotypic studies on 25 of 45 clones revealed that 19 (76%) of the clones were CD4+, 5 (20%) were CD8+, and 1 (4%) clone was CD4- and CD8-. Twenty-three clones were stained for identification of T-cell receptors. Twenty-two clones expressed alpha/beta T-cell receptors and one clone (CD4-/CD8-) expressed no T-cell receptor. Nineteen clones (42%) were autoreactive with no restriction to class I or class II HLA antigens. By contrast, proliferation of two of the seven clones was inhibited by class I monoclonal antibody, whereas proliferation of four of seven clones was inhibited by class II monoclonal antibody.


CONCLUSIONS
These data suggest that skin-infiltrating lymphocytes in patients with psoriasis may recognize HLA-associated molecules, perhaps the peptide of the HLA groove. The recognition of the peptide is presumably inhibited when monoclonal antibody is bound to the HLA molecule.
Nikaein
A
A
Department of Pathology, Baylor University Medical Center, Dallas, TX 75246.
Morris
L
L
Phillips
C
C
Soliman
M
M
Ordonez
G
G
Silverman
A
A
Stone
M J
MJ
Menter
A
A
D015704:CD4 Antigens / Q000032:analysis; D016827:CD8 Antigens / Q000032:analysis; D002999:Clone Cells / Q000276:immunology / Q000473:pathology; D006680:HLA Antigens / Q000032:analysis; D006682:HLA-DP Antigens / Q000032:analysis; D006801:Humans; D016130:Immunophenotyping; D007694:Killer Cells, Natural / Q000473:pathology; D011565:Psoriasis / Q000473:pathology*; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000032:analysis; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000032:analysis; D012867:Skin / Q000473:pathology*; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000473:pathology*
D015704:CD4 Antigens; D016827:CD8 Antigens; D006680:HLA Antigens; D006682:HLA-DP Antigens; D016693:Receptors, Antigen, T-Cell, alpha-beta; D016692:Receptors, Antigen, T-Cell, gamma-delta