title: Aromatic diamidines are reversible inhibitors of porcine kidney diamine oxidase.
abstract: The inhibitory ability of aromatic diamidines has been studied on porcine kidney diamine oxidase. The reversibility of drug-protein interactions has been tested by means of exhaustive dialysis experiments, showing in all cases a reversible binding pattern. Ki values obtained by means of Lineweaver-Burk plots were: stilbamidine 12 microM, 2-OH-stilbamide 8.5 microM, phenamidine 4 microM, propamidine 8 microM, dibromopropamidine 4.9 microM and amicarbalide 12 microM.
authors: Departamento de Fisiología, Farmacología y Toxicología, Facultad de Veterinaria, Universidad de León, Spain.
authors: J C
authors: JC
authors: Cubría
authors: R
authors: R
authors: Balaña Fouce
authors: M L
authors: ML
authors: Alvarez-Bujidos
authors: A
authors: A
authors: Negro
authors: A I
authors: AI
authors: Ortiz
authors: D
authors: D
authors: Ordóñez
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000188:drug therapy; D006631:Amine Oxidase (Copper-Containing) / Q000037:antagonists & inhibitors*; D000818:Animals; D001550:Benzamidines / Q000494:pharmacology; D001665:Binding Sites; D004133:Diminazene / Q000031:analogs & derivatives* / Q000494:pharmacology; D007668:Kidney / Q000187:drug effects* / Q000201:enzymology; D007700:Kinetics; D010419:Pentamidine / Q000494:pharmacology*; D013266:Stilbamidines / Q000494:pharmacology; D013552:Swine
chemical_list: D001550:Benzamidines; D013266:Stilbamidines; C001787:phenamidine; D010419:Pentamidine; D006631:Amine Oxidase (Copper-Containing); C005555:propamidine; C003915:diminazene aceturate; C042560:stilbamidine; D004133:Diminazene