PLA2 activity in rat liver nuclei.
1. Subcellular fractions of rat liver were assayed for PLA2 activity. 2. The PLA2 assay measures the release of [3H]oleic acid from phospholipids, using labeled E. coli as substrate. 3. Nuclear fractions contained PLA2 activity, which was Ca2+ dependent and could not be explained from mitochondria, microsomal or plasma membrane contamination. 4. The Vmax value of nuclear PLA2 is 0.30 +/- 0.04 pmol oleic acid/min/mg protein; its Km value is 0.86 +/- 0.12 microM, similar to that of mitochondrial PLA2. 5. We conclude that rat liver nuclei contain PLA2 activity.
van der Klis
F R
FR
Department of Endocrinology, Academish Medisch Centrum, Amsterdam, The Netherlands.
Wiersinga
W M
WM
D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002458:Cell Fractionation; D002467:Cell Nucleus / Q000201:enzymology*; D004492:Edetic Acid / Q000494:pharmacology; D004533:Egtazic Acid / Q000494:pharmacology; D004546:Elapid Venoms / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism; D008099:Liver / Q000201:enzymology* / Q000648:ultrastructure; D008297:Male; D019301:Oleic Acid; D009829:Oleic Acids / Q000378:metabolism; D010741:Phospholipases A / Q000378:metabolism*; D054467:Phospholipases A2; D051381:Rats; D017208:Rats, Wistar
D004546:Elapid Venoms; D009829:Oleic Acids; D019301:Oleic Acid; D004533:Egtazic Acid; D004492:Edetic Acid; D010741:Phospholipases A; D054467:Phospholipases A2; D002118:Calcium