title: [Estimation of proliferative activity of experimental tongue papillomas and carcinomas in rats--comparison with DNA cytofluorometric analysis and silver-stained nucleolar organizer regions].
abstract: Eleven tongue papillomas and 25 tongue carcinomas were produced in rats by the oral administration of 0.001% 4-nitroquinoline 1-oxide (4 NQO) in drinking water, and tumor kinetics were investigated by both DNA cytofluorometry and Ag-nucleolar organizer regions (Ag-NORs) staining. The histopathologic characteristics, the mean Ag-NOR number per cell and DNA ploidy patterns were compared. The mean Ag-NOR number was least in normal epithelium, more numerous in papillomas and highest in squamous cell carcinomas. The differences were statistically significant. All cases of normal epithelium and papilloma showed a diploid pattern. Eighteen cases of the squamous cell carcinomas (72%) showed a diploid pattern, and 7 cases (28%) exhibited a diploid plus tetraploid pattern. There was a significant difference in the mean Ag-NOR number between the two groups of squamous cell carcinomas. These results suggest that the mean Ag-NOR number may reflect the cell kinetics in the process of carcinogenesis and polyploidization of carcinomas.
authors: Dept. of Otolaryngology, Osaka Medical College.
authors: T
authors: T
authors: Itoh
authors: Y
authors: Y
authors: Yamamoto
authors: T
authors: T
authors: Saka
authors: H
authors: H
authors: Takahashi
mesh_terms: D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000473:pathology*; D002455:Cell Division; D004273:DNA, Neoplasm / Q000032:analysis* / Q000235:genetics; D005434:Flow Cytometry; D008297:Male; D009697:Nucleolus Organizer Region / Q000473:pathology*; D010212:Papilloma / Q000235:genetics / Q000473:pathology*; D011003:Ploidies; D051381:Rats; D017207:Rats, Sprague-Dawley; D016622:Silver Staining; D014062:Tongue Neoplasms / Q000235:genetics / Q000473:pathology*
chemical_list: D004273:DNA, Neoplasm