Microsomal alkaline phosphatase activity in retinol deficiency induced albino rats.
The investigations showed a significant decrease in the alkaline phosphatase activity of retinol deficient liver (48.6%), kidney (65.8%) and spleen (61.9%), as compared to the controls (100%). An increase in Vmax and Km by 12 to 51.5% and 90.4 to 189%, respectively, was observed in all the tissues in the retinol deficient group, as compared to the controls. Subsequent freezing and thawing reduced the activity of alkaline phosphatase by 22.5 to 35.8% in the experimental group; whereas the reduction in the control group ranged from 8.8 to 21.5%. In the presence of lectins and detergents the activity of alkaline phosphatase decreased in both the groups to different levels.
Kaul
S
S
Department of Nutrition and Food Safety, Central Food Technological Research Institute (CFTRI), Mysore, India.
Krishnakantha
T P
TP
D000469:Alkaline Phosphatase / Q000378:metabolism*; D000818:Animals; D003208:Concanavalin A / Q000494:pharmacology; D003902:Detergents; D005615:Freezing; D006023:Glycoproteins / Q000378:metabolism; D007668:Kidney / Q000201:enzymology; D008099:Liver / Q000201:enzymology; D008297:Male; D008861:Microsomes / Q000187:drug effects / Q000201:enzymology*; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology; D010835:Phytohemagglutinins / Q000494:pharmacology; D051381:Rats; D013154:Spleen / Q000201:enzymology; D014802:Vitamin A Deficiency / Q000201:enzymology*
D003902:Detergents; D006023:Glycoproteins; D010835:Phytohemagglutinins; D003208:Concanavalin A; D000469:Alkaline Phosphatase