title: Hydraulic conductivity of ischemic pulmonary venules.
abstract: We report the first determination of lung endothelial barrier properties in ischemic, nonreperfused microvessels. We quantified the endothelial barrier in terms of hydraulic conductivity (Lp) in single pulmonary venules (diameter 20-50 microns) of isolated blood perfused lungs (dog, rat), held at constant inflation pressure (5 cmH2O) with a gas mixture containing 21% oxygen. Lp were determined by our split-drop technique in which an oil drop is first microinjected into a venule and then split by microinjection of a protein solution. Lp was interpreted from measurements of the rate of oil drop movement. Baseline Lp recorded in the first 30 min of perfusion averaged 3.4 +/- 0.9 x 10(-7) ml/(cm2.s.cmH2O). Then, in two separate groups of venules in which we established 1.3 +/- 0.1 h and 3.4 +/- 0.8 h of ischemia, we determined Lp which were, respectively, 145 +/- 6.5 and 308 +/- 13% above baseline (P < 0.05). We conclude that ischemia alone, in the absence of reperfusion, significantly deteriorates the lung endothelial barrier.
authors: Department of Medicine, College of Physicians and Surgeons, Columbia University, St. Luke's-Roosevelt Hospital Center, New York, New York 10019.
authors: R L
authors: RL
authors: Qiao
authors: R
authors: R
authors: Sadurski
authors: J
authors: J
authors: Bhattacharya
mesh_terms: D000818:Animals; D004285:Dogs; D004730:Endothelium, Vascular / Q000503:physiopathology*; D007511:Ischemia / Q000503:physiopathology*; D008168:Lung / Q000098:blood supply*; D009131:Muscle, Smooth, Vascular / Q000503:physiopathology*; D010539:Permeability; D011652:Pulmonary Circulation; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000503:physiopathology; D014699:Venules / Q000503:physiopathology*