2',5'-Oligoadenylate-dependent RNase L is a dimer of regulatory and catalytic subunits.
The subunit composition of RNase L, a key enzyme in the interferon system, has been characterized. RNase L was purified from human Daudi cells on a column of 2-5A-Sepharose and used to immunize Balb/c mice. A specific monoclonal antibody which recognizes a protein of 80 kDa has been isolated. This protein has been characterized and shown to be an RNA-binding protein with nuclease activity which is associated with, but distinct from, the 80-kDa 2-5A-binding protein known previously as RNase L. It is therefore proposed that the 2-5A-dependent RNase L is a complex of two distinct subunits: an 80-kDa RNA-binding protein (i.e. the catalytic subunit) and an 80-kDa 2-5A-binding protein (i.e. the regulatory subunit).
Salehzada
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Laboratoire de Biochimie des Protéines, URA Centre National de la Recherche Scientifique 1191, Université de Montpellier II Sciences et Techniques du Languedoc, France.
Silhol
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M
Steff
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AM
Lebleu
B
B
Bisbal
C
C
D000227:Adenine Nucleotides / Q000378:metabolism / Q000494:pharmacology*; D000818:Animals; D000911:Antibodies, Monoclonal; D002846:Chromatography, Affinity; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D004722:Endoribonucleases / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D004789:Enzyme Activation; D006801:Humans; D007700:Kinetics; D046911:Macromolecular Substances; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology; D008954:Models, Biological; D008970:Molecular Weight; D009843:Oligoribonucleotides / Q000378:metabolism / Q000494:pharmacology*; D013347:Subcellular Fractions / Q000201:enzymology; D014407:Tumor Cells, Cultured
D000227:Adenine Nucleotides; D000911:Antibodies, Monoclonal; D046911:Macromolecular Substances; D009843:Oligoribonucleotides; C023505:2',5'-oligoadenylate; D004722:Endoribonucleases; C031956:2-5A-dependent ribonuclease