Isolation and purification of AAL-toxin from Alternaria alternata grown on rice.
An isolation and purification procedure is described for AAL-toxin, a secondary metabolite produced by Alternaria alternata. The method involves growth of the fungus on rice media, extraction with chloroform followed by methanol: water from fungus-infested rice, and purification of the aqueous layer that contains AAL-toxin by using chromatography methods. The AAL-toxin was of type A, and white in color, and its purity was > or = 95% with mol. wt 522 (M + 1).
Abbas
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HK
USDA/ARS/Southern Weed Science Laboratory, Stoneville, MS 38776.
Vesonder
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RF
D000528:Alternaria / Q000737:chemistry*; D002855:Chromatography, Thin Layer; D003470:Culture Media; D009183:Mycotoxins / Q000302:isolation & purification*; D012275:Oryza; D013110:Sphingosine
C047996:Alternaria alternata pathotoxin TA; D003470:Culture Media; D009183:Mycotoxins; D013110:Sphingosine