title: In situ hybridization analysis of developmental stages of Pneumocystis carinii that are transcriptionally active for a major surface glycoprotein gene.
abstract: An abundant glycoprotein on the surface of Pneumocystis carinii, termed gpA or gp120, is thought to play a role in the interaction of this opportunistic pathogen with its host. Using RNA:RNA hybridization techniques, the in situ expression of gpA mRNA in developmental forms of the organism was investigated in a ferret model of P. carinii pneumonia. The results suggested that the relative abundance of gpA-specific mRNA was variable in different developmental stages of ferret P. carinii. P. carinii localized along the epithelial lining of alveoli were transcriptionally active. Immunocytochemical detection of gpA and Giemsa staining suggested that many of these organisms were trophic forms of P. carinii. While no detectable gpA mRNA signal was found in the majority of P. carinii cysts, a portion of identifiable cysts co-localized with significant levels of gpA mRNA signal. Differential staining of the cyst wall with Gomori's methenamine silver suggested that the transcriptionally active P. carinii cysts were the intermediate or precyst forms of the organism, while the cysts with no detectable mRNA signal were either mature or empty (excysted). Alveolar macrophages were observed surrounded by transcriptionally active organisms; however, no gpA-transcriptional activity was detected within macrophages. Taken together, the results suggest that transcription of gpA occurs in forms of P. carinii that are actively replicating, and in close proximity or contact with, alveolar epithelial cells.
authors: Department of Medicine/Hematology Unit, University of Rochester School of Medicine and Dentistry, New York 14642.
authors: P J
authors: PJ
authors: Haidaris
authors: T W
authors: TW
authors: Wright
authors: F
authors: F
authors: Gigliotti
authors: M A
authors: MA
authors: Fallon
authors: A A
authors: AA
authors: Whitbeck
authors: C G
authors: CG
authors: Haidaris
mesh_terms: D000818:Animals; D005289:Ferrets; D005656:Fungal Proteins / Q000096:biosynthesis* / Q000235:genetics; D015966:Gene Expression Regulation, Fungal; D017403:In Situ Hybridization; D016676:Macrophages, Alveolar / Q000502:physiology; D008297:Male; D011010:Pneumocystis / Q000235:genetics / Q000254:growth & development*; D011020:Pneumonia, Pneumocystis / Q000382:microbiology; D012331:RNA, Fungal / Q000096:biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis; D012098:Reproduction; D014158:Transcription, Genetic
chemical_list: D005656:Fungal Proteins; D012331:RNA, Fungal; D012333:RNA, Messenger