[Effect of medroxyprogesterone acetate on the anticellular activity of 5-fluorouracil against human breast and stomach cancer cells].
As 5-fluorouracil (5-FU) has been known to show clinically antitumor effects against both breast and stomach carcinomas. We compared the combined effects of medroxyprogesterone acetate (MPA) with 5-FU on the growth of cultured human breast and stomach carcinoma cells. MPA inhibited the growth of estrogen-dependent human breast carcinoma MCF-7 cells at the low concentrations and exhibited an additive effect in combination with 5-FU. MPA also inhibited the growth of human stomach carcinoma MKN-45 cells at relatively high concentrations and exhibited an additive effect in combination with 5-FU. Human stomach carcinoma MKN-28 cells were rather insensitive to MPA, but, an additive combination effect of MPA and 5-FU was observed. These three cell lines were found to have MPA-binding proteins which may be distinct from the nuclear progesterone receptor, suggesting the correlation with growth-inhibitory activity of MPA.
Ishida
H
H
Pharmaceutical Research Laboratories, Kyowa Hakko Kogyo Co., Ltd.
Okabe
M
M
Gomi
K
K
Horiuchi
R
R
D001943:Breast Neoplasms / Q000737:chemistry / Q000473:pathology*; D002455:Cell Division / Q000187:drug effects; D004357:Drug Synergism; D004967:Estrogens / Q000502:physiology*; D005260:Female; D005472:Fluorouracil / Q000494:pharmacology*; D006801:Humans; D017258:Medroxyprogesterone Acetate / Q000494:pharmacology*; D009376:Neoplasms, Hormone-Dependent / Q000737:chemistry / Q000473:pathology*; D011960:Receptors, Estrogen / Q000032:analysis; D011980:Receptors, Progesterone / Q000032:analysis; D013274:Stomach Neoplasms / Q000737:chemistry / Q000473:pathology*; D014407:Tumor Cells, Cultured / Q000187:drug effects
D004967:Estrogens; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone; D017258:Medroxyprogesterone Acetate; D005472:Fluorouracil