Two developmentally regulated mRNAs encoding actin-binding proteins in Physarum polycephalum.
A cDNA library from amoebae of Physarum polycephalum was screened by differential hybridization. Two clones contained inserts for mRNAs present in amoebae and absent in plasmodia. The LAV3-4 cDNA encodes a 402 aa protein (ABP-46) that shows sequence similarity to the actin binding site in the N-terminal region of the alpha-actinin family. The LAV3-5 cDNA is 76% identical to the Dictyostelium actin bundling protein, which cross-links and stabilizes actin filaments in amoebal filopodia.
St-Pierre
B
B
Départment de Biologie, Faculté des Sciences et Génie, Université Laval, Québec, Canada.
Couture
C
C
Laroche
A
A
Pallotta
D
D
D000185:Actinin / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004023:Dictyostelium / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D016839:Physarum polycephalum / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis*
D005656:Fungal Proteins; D012333:RNA, Messenger; D000185:Actinin