The NF-kappa B binding site is necessary for efficient replication of simian immunodeficiency virus of macaques in primary macrophages but not in T cells in vitro.
We demonstrate here that the nuclear factor-kappa B (NF-kappa B) binding site in the simian immunodeficiency virus (SIVmac) long terminal repeat is essential for efficient virus replication in primary alveolar macrophages but dispensable for efficient replication in primary T cells. Mutation of the NF-kappa B site does not seriously impair replication of a T-cell-tropic SIVmac239 or a macrophagetropic SIVmacEm* in peripheral blood lymphocytes or established CD4+ cell lines; however, mutation of the NF-kappa B site prevents efficient SIVmacEm* replication in primary alveolar macrophages. These data suggest that efficient replication in primary macrophages requires both envelope and long terminal repeat determinants.
Bellas
R E
RE
Center for Cancer Research, Massachusetts Institute of Technology, Cambridge 02139.
Hopkins
N
N
Li
Y
Y
D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D003588:Cytopathogenic Effect, Viral; D008251:Macaca; D008264:Macrophages / Q000166:cytology / Q000382:microbiology*; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D016328:NF-kappa B / Q000378:metabolism*; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics*; D015302:Simian Immunodeficiency Virus / Q000254:growth & development*; D013601:T-Lymphocytes / Q000166:cytology / Q000382:microbiology*; D014779:Virus Replication
D016328:NF-kappa B