title: Altered platelet function associated with the bronchial hyperresponsiveness accompanying nocturnal asthma.
abstract: BACKGROUND
  Nocturnal awakening is a common feature of bronchial asthma, and yet the mechanisms underlying this phenomenon are poorly understood. We investigated whether nocturnal awakening is associated with changes in platelet function with the use of a variety of markers of platelet activation.
  
  
  METHODS
  Ten patients with a history of nocturnal asthma and 10 age- and sex-matched healthy control subjects were studied at 10:00 PM, 4:00 AM, and 10:00 AM on 2 consecutive days. The following parameters were tested: forced expiratory volume in 1 second (FEV1), log dose of methacholine inducing a 20% fall in FEV1, platelet count and volume, platelet aggregation induced by collagen or activating factor, and plasma and intraplatelet levels of beta-thromboglobulin and platelet factor 4.
  
  
  RESULTS
  We have demonstrated that altered platelet function and platelet activation occurs at 4:00 AM in patients with nocturnal asthma and is associated with the maximum increases in bronchial reactivity. Such changes were not observed in 10 control subjects. Platelet dysfunction has been detected as a reduced aggregatory response of platelets to collagen and platelet activating factor such that up to 5 times more platelet activating factor and 1.5 times more collagen were required to elicit a threshold aggregatory response in asthmatic subjects when compared with control subjects; this difference was evident at all time points tested. Furthermore, at 4:00 AM there were significantly lower levels of intraplatelet beta-thromboglobulin corresponding to the maximum reduction in peak expiratory flow and to the maximal increase in bronchial responses to inhaled methacholine.
  
  
  CONCLUSIONS
  These results suggest that platelet activation accompanies nocturnal asthma and further suggest that platelets may play a role in this common clinical condition.
authors: International Inter-University Centre on Thrombosis and Haemostasis, Italy.
authors: P
authors: P
authors: Gresele
authors: M
authors: M
authors: Dottorini
authors: M L
authors: ML
authors: Selli
authors: L
authors: L
authors: Iannacci
authors: S
authors: S
authors: Canino
authors: T
authors: T
authors: Todisco
authors: S
authors: S
authors: Romano
authors: P
authors: P
authors: Crook
authors: C P
authors: CP
authors: Page
authors: G G
authors: GG
authors: Nenci
mesh_terms: D000328:Adult; D001249:Asthma / Q000097:blood* / Q000503:physiopathology; D001980:Bronchi / Q000187:drug effects / Q000503:physiopathology*; D002940:Circadian Rhythm; D005260:Female; D006801:Humans; D008297:Male; D016210:Methacholine Chloride / Q000494:pharmacology; D015539:Platelet Activation; D010976:Platelet Count; D010978:Platelet Factor 4 / Q000032:analysis; D001620:beta-Thromboglobulin / Q000032:analysis
chemical_list: D001620:beta-Thromboglobulin; D016210:Methacholine Chloride; D010978:Platelet Factor 4