A light-scattering method for measuring the sizes of insoluble immune complexes.
A turbidimetric method for measuring the diameters of insoluble immune complexes, based on the wavelength dependence of their ability to scatter light, was developed. The method was validated by demonstrating that it gave experimental values for the diameters of polystyrene microspheres which were in good agreement with independently known values of these. The method was used to measure the diameters of ovalbumin:anti-ovalbumin IgG immune complexes, giving values consistent with literature measurements of the sizes of other IgG-containing immune complexes.
Easterbrook-Smith
S B
SB
Department of Biochemistry, University of Sydney, NSW, Australia.
D000936:Antigen-Antibody Complex / Q000737:chemistry*; D008027:Light; D046911:Macromolecular Substances; D012542:Scattering, Radiation; D012995:Solubility
D000936:Antigen-Antibody Complex; D046911:Macromolecular Substances