Time-resolved and equilibrium measurements of the effects of poly(ethylene glycol) on small unilamellar phospholipid vesicles.
The effects of poly(ethylene glycol) (PEG) on sonicated unilamellar vesicles made of phosphatidylcholine have been examined. Stopped-flow and equilibrium data are presented for vesicle aggregation, vesicle leakage, lipid mixing, and aqueous contents mixing. Vesicle aggregation is detected as a monoexponential increase in light scattering, for PEG concentrations between 5 and 10%. In the region between 10-15% and 23-27% PEG, under our experimental conditions, the increase in light scattering follows a more complex biexponential kinetics, and, under these conditions, vesicle aggregation is accompanied by lipid mixing, a combination of events denoted as "close apposition". Above 23-27% PEG, the increase in light scattering is accompanied by fast lipid mixing, and also mixing of aqueous contents, all this being indicative of vesicle fusion; in addition, leakage occurs under these circumstances. Fusion takes place at high PEG concentrations, as indicated above, without any dilution step. From a methodological point of view, the Tb/DPA assay is shown to be more appropriate than the ANTS/DPX method for leakage and fusion studies in the presence of PEG.
Viguera
A R
AR
Department of Biochemistry, University of the Basque Country, Bilbao, Spain.
Mencía
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M
Goñi
F M
FM
D004735:Energy Transfer; D007700:Kinetics; D008027:Light; D008081:Liposomes / Q000737:chemistry* / Q000378:metabolism; D008561:Membrane Fusion; D009281:Naphthalenes; D010713:Phosphatidylcholines / Q000737:chemistry; D010848:Picolinic Acids; D011092:Polyethylene Glycols / Q000494:pharmacology*; D011726:Pyridinium Compounds; D012542:Scattering, Radiation; D013050:Spectrometry, Fluorescence; D013725:Terbium; D014992:Xylenes
D008081:Liposomes; D009281:Naphthalenes; D010713:Phosphatidylcholines; D010848:Picolinic Acids; D011726:Pyridinium Compounds; D014992:Xylenes; D013725:Terbium; D011092:Polyethylene Glycols; C046465:8-amino-1,3,6-naphthalenetrisulfonic acid; C004860:dipicolinic acid