title: Isolation and structure of the marine sponge cell growth inhibitory cyclic peptide phakellistatin 1.
abstract: A new cell growth inhibitory (P-388 murine leukemia ED50 7.5 micrograms/ml) cycloheptapeptide designated phakellistatin 1 was isolated from two Indo-Pacific sponges, Phakellia costata and Stylotella aurantium. Structural elucidation was accomplished utilizing high field nmr, amino acid analyses, and mass spectral techniques (fab, tandem ms/ms), followed by chiral gas chromatographic procedures for absolute configuration assignments (all S-amino acid units). By these methods phakellistatin 1 [1] was found to be cyclo (Pro-Ile-Pro-Ile-Phe-Pro-Tyr), and this assignment was finally confirmed by an X-ray crystal structure determination.
authors: Department of Chemistry, Arizona State University, Tempe 85287-1604.
authors: G R
authors: GR
authors: Pettit
authors: Z
authors: Z
authors: Cichacz
authors: J
authors: J
authors: Barkoczy
authors: A C
authors: AC
authors: Dorsaz
authors: D L
authors: DL
authors: Herald
authors: M D
authors: MD
authors: Williams
authors: D L
authors: DL
authors: Doubek
authors: J M
authors: JM
authors: Schmidt
authors: L P
authors: LP
authors: Tackett
authors: D C
authors: DC
authors: Brune
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D003460:Crystallization; D007941:Leukemia P388 / Q000188:drug therapy; D051379:Mice; D008969:Molecular Sequence Data; D010456:Peptides, Cyclic / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D011161:Porifera / Q000737:chemistry*; D017433:Protein Structure, Secondary; D014961:X-Ray Diffraction
chemical_list: D000970:Antineoplastic Agents; D010456:Peptides, Cyclic; C080583:phakellistatin 1