Characterization of N-ethoxycarbonyl ethyl esters of amino acids by mass spectrometry.
Derivatization of amino acids by using ethyl chloroformate-ethanol-pyridine provides volatile products, N-ethoxycarbonyl amino acid ethyl esters (ECEEs), which are easily amenable to GC or GC-MS analysis. MS behavior of these compounds under electron-impact has been studied. The fragments observed in the spectra facilitate recognition of commonly occurring protein amino acids and characterization of unknown analogues.
Huang
Z H
ZH
Department of Biochemistry, Michigan State University, East Lansing 48824-1319.
Wang
J
J
Gage
D A
DA
Watson
J T
JT
Sweeley
C C
CC
Husek
P
P
D000596:Amino Acids / Q000737:chemistry*; D004952:Esters / Q000737:chemistry; D013058:Mass Spectrometry
D000596:Amino Acids; D004952:Esters