Ascorbic acid phosphate ester and wound healing in rabbit corneal alkali burns: epithelial basement membrane and stroma.
We examined the effect of L-ascorbic acid 2-phosphate (P-Asc) on the healing of alkali-burned corneas in rabbits. Round filter paper containing 1 N NaOH was applied to the central cornea for 60 or 120 s to produce the alkali burn. Animals were treated with topical saline, 10% ascorbate, or 6.5% P-Asc applied on the cornea. The corneas were then examined histologically. Burned stroma showed no toluidine blue staining, indicating a loss of glycosaminoglycan. In the 60-s burn group, P-Asc reduced the size of the unstained area as compared with the control. Transmission electron microscopy showed basal lamina under new epithelia in the corneas treated with ascorbate or P-Asc, but not in controls. These observations support the theory that P-Asc may have a therapeutic role in the repair of corneal alkali burns.
Saika
S
S
Department of Ophthalmology, Wakayama Medical College, Japan.
Uenoyama
K
K
Hiroi
K
K
Tanioka
H
H
Takase
K
K
Hikita
M
M
D000287:Administration, Topical; D000818:Animals; D001205:Ascorbic Acid / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000494:pharmacology; D001485:Basement Membrane / Q000187:drug effects* / Q000648:ultrastructure; D002057:Burns, Chemical / Q000188:drug therapy / Q000473:pathology; D003315:Cornea / Q000187:drug effects* / Q000648:ultrastructure; D065306:Corneal Injuries; D003319:Corneal Stroma / Q000187:drug effects* / Q000648:ultrastructure; D005126:Eye Burns / Q000139:chemically induced / Q000473:pathology; D005260:Female; D008297:Male; D009883:Ophthalmic Solutions / Q000008:administration & dosage; D011817:Rabbits; D012972:Sodium Hydroxide; D014945:Wound Healing / Q000187:drug effects*
D009883:Ophthalmic Solutions; C011669:ascorbate-2-phosphate; D012972:Sodium Hydroxide; D001205:Ascorbic Acid