Nicotinic acetylcholine receptor in dissociated rat nucleus tractus solitarii neurons.
The ACh-activated response of the acutely dissociated neuron from the rat nucleus tractus solitarii (NTS) was investigated using conventional and perforated-patch techniques. In the present preparation, ACh and nicotine evoked inward transient currents in approximately 30% of NTS neurons tested. The ACh-activated inward current reversed the direction near 0 mV and was inhibited by D-tubocurarine in a dose-dependent manner. In contrast, muscarine resulted in no detectable changes in the NTS neurons. Some populations of the NTS had nicotinic but no muscarinic acetylcholine receptors.
Ueno
S
S
Department of Neurophysiology, Tohoku University School of Medicine, Sendai, Japan.
Kakehata
S
S
Akaike
N
N
D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D004305:Dose-Response Relationship, Drug; D004594:Electrophysiology / Q000379:methods; D066298:In Vitro Techniques; D008526:Medulla Oblongata / Q000166:cytology / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism* / Q000502:physiology; D009538:Nicotine / Q000037:antagonists & inhibitors / Q000494:pharmacology; D051381:Rats; D011978:Receptors, Nicotinic / Q000378:metabolism*; D014403:Tubocurarine / Q000494:pharmacology
D011978:Receptors, Nicotinic; D009538:Nicotine; D000109:Acetylcholine; D014403:Tubocurarine