title: Activated natural killer cells suppress myelopoiesis in mice with severe combined immunodeficiency.
abstract: The in vivo effect of natural killer (NK) cell activation on autologous myelopoiesis was studied in an environment deficient of functional T and B cells. Administration of 3,6-bis[2-(Dimethylamino)-ethoxy]-9H-xanthen-9-one dihydrochloride) Tilorone) or recombinant interleukin-2 (rIL-2) to mice with severe combined immunodeficiency (C.B.-17 scid/scid) resulted in an increase in YAC-1 lysis by their splenocytes as well as bone marrow cells. Recombinant IL-2 furthermore led to a fivefold increase in the cellularity of the spleen. When assayed against human NK/lymphokine-activated killer (LAK) target, K562 cell line, the IL-2-activated mouse cells exhibited no cytotoxicity across the species barrier. Both agents induced a profound suppression of myelopoietic progenitor cells as measured in a 7-day granulocyte-macrophage colony forming cell (GM-CFC) assay. We conclude that the presence of neither functional T nor B cells is necessary for NK cells to mediate inhibition of myelopoiesis in the autologous host.
authors: Department of Immunology, Karolinska Institute, Stockholm, Sweden.
authors: P
authors: P
authors: Pisa
authors: E
authors: E
authors: Sitnicka
authors: M
authors: M
authors: Hansson
mesh_terms: D000818:Animals; D001853:Bone Marrow / Q000276:immunology; D015672:Erythroid Precursor Cells / Q000187:drug effects*; D006801:Humans; D007376:Interleukin-2 / Q000276:immunology* / Q000494:pharmacology; D015979:Killer Cells, Lymphokine-Activated / Q000276:immunology*; D051379:Mice; D016513:Mice, SCID; D008938:Mitosis / Q000187:drug effects; D011994:Recombinant Proteins / Q000494:pharmacology; D013154:Spleen / Q000187:drug effects / Q000276:immunology*; D013994:Tilorone / Q000494:pharmacology*
chemical_list: D007376:Interleukin-2; D011994:Recombinant Proteins; D013994:Tilorone