title: HSP86 and HSP84 exhibit cellular specificity of expression and co-precipitate with an HSP70 family member in the murine testis.
abstract: This study extends to the protein level our previous observations, which had established the stage and cellular specificity of expression of hsp86 and hsp84 in the murine testis in the absence of exogenous stress. Immunoblot analysis was used to demonstrate that HSP86 protein was present throughout testicular development and that its levels increased with the appearance of differentiating germ cells. HSP86 was most abundant in the germ cell population and was present at significantly lower levels in the somatic cells. By contrast, the HSP84 protein was detected in the somatic cells of the testis rather than in germ cells. The steady-state levels of HSP86 and HSP84 paralleled the pattern of the expression of their respective mRNAs, suggesting that regulation at the level of translation was not a major mechanism controlling hsp90 gene expression in testicular cells. Immunoprecipitation analysis revealed that a 70-kDa protein coprecipitated with the HSP86/HSP84 proteins in testicular homogenates. This protein was identified as an HSP70 family member by immunoblot analysis, suggesting that HSP70 and HSP90 family members interact in testicular cells.
authors: Department of Genetics, Columbia University College of Physicians and Surgeons, New York, NY 10032.
authors: C M
authors: CM
authors: Gruppi
authors: D J
authors: DJ
authors: Wolgemuth
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D005786:Gene Expression Regulation; D006360:Heat-Shock Proteins / Q000032:analysis* / Q000235:genetics / Q000502:physiology; D015151:Immunoblotting; D008297:Male; D051379:Mice; D008970:Molecular Weight; D009928:Organ Specificity; D011233:Precipitin Tests; D014176:Protein Biosynthesis; D013090:Spermatocytes / Q000737:chemistry*; D013091:Spermatogenesis; D013737:Testis / Q000737:chemistry* / Q000166:cytology / Q000254:growth & development
chemical_list: D006360:Heat-Shock Proteins