In vitro and ex vivo responses of bovine lymphocytes to hypodermin C, an enzyme secreted by Hypoderma lineatum (insect oestridae).
The aim of this work was to determine if Hypodermin C (HC), an enzyme secreted by Hypoderma spp. larvae during their migration, is able to depress the lymphocyte response to mitogens and antigens. HC, when added to the lymphocyte culture, was able to influence the response of naive-calf lymphocytes to phytohemagglutinin. Secondly, injections of HC to uninfested and previously-infested cattle did not modify the proliferative response of the lymphocytes of these animals to mitogens. Thirdly, injections of HC to infested cattle did not negatively influence the specific humoral and cellular responses.
Chabaudie
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N
Laboratoire d'Immunopathologie des Maladies Parasitaires, INRA, Nouzilly, France.
Boulard
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C
D000818:Animals; D000917:Antibody Formation / Q000187:drug effects; D000941:Antigens / Q000276:immunology; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology*; D002478:Cells, Cultured; D003208:Concanavalin A; D004305:Dose-Response Relationship, Drug; D005260:Female; D007000:Hypodermyiasis / Q000276:immunology / Q000662:veterinary*; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000187:drug effects*; D010835:Phytohemagglutinins; D011043:Pokeweed Mitogens; D012697:Serine Endopeptidases / Q000494:pharmacology*; D013997:Time Factors
D000941:Antigens; D010835:Phytohemagglutinins; D011043:Pokeweed Mitogens; D003208:Concanavalin A; D012697:Serine Endopeptidases; C070019:hypodermin C