title: Cell surface carbohydrates modulate neutrophil adherence to alveolar type II cells in vitro.
abstract: We characterized the influence of phosphorylated sugars and cell surface sialic acids on the adherence of human polymorphonuclear leukocyte (PMN) to rat alveolar type II cell (ATII cells) and human-derived A549 cell monolayers in vitro. Percent adherence of radiolabeled polymorphonuclear leukocytes was assessed after incubating cells with the carbohydrates, enzymes, or lectins to be tested. Lactose-1-phosphate (Lact1P) and maltose-1-phosphate (Malt1P) (10 mM) inhibited adherence of PMN to ATII cells and A549 cells. Maximal inhibition followed treatment of both PMN and rat ATII cells and amounted to 85 +/- 7% with Lact1P and 92 +/- 3% with Malt1P. Inhibition was concentration dependent. Incubation of PMN with mannose-6-phosphate reduced adherence to rat ATII cells and A549 cells by 36 +/- 11 and 39 +/- 8%, respectively. Maximal concentrations of sugars did not alter cellular viability. Neuraminidase-induced desialilation of ATII cells increased adherence of PMN by 36 +/- 7% to rat ATII cells and by 86 +/- 18% to A549 cells. Masking of terminal sialic acids on rat ATH cells with Limulus polyphemus agglutinin (100 micrograms/ml) increased adherence by 50 +/- 2%. These results indicate that cell surface carbohydrates are involved in the regulation of the adhesive interaction between PMN and ATII cells in vitro.
authors: Institut National de la Santé et de la Recherche Médicale U. 226, Paris, France.
authors: B
authors: B
authors: Crestani
authors: C
authors: C
authors: Rolland
authors: A
authors: A
authors: Petiet
authors: N
authors: N
authors: Colas-Linhart
authors: M
authors: M
authors: Aubier
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology*; D002462:Cell Membrane / Q000502:physiology / Q000648:ultrastructure; D002478:Cells, Cultured; D004847:Epithelial Cells; D004848:Epithelium / Q000502:physiology / Q000648:ultrastructure; D006801:Humans; D007700:Kinetics; D008175:Lung Neoplasms; D008297:Male; D008360:Mannosephosphates / Q000494:pharmacology; D008854:Microscopy, Electron; D009504:Neutrophils / Q000502:physiology*; D011650:Pulmonary Alveoli / Q000166:cytology / Q000502:physiology* / Q000648:ultrastructure; D051381:Rats; D017207:Rats, Sprague-Dawley; D013403:Sugar Phosphates / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
chemical_list: D008360:Mannosephosphates; D013403:Sugar Phosphates; C080751:maltose 1-phosphate; C080750:lactose 1-phosphate; C027693:mannose-6-phosphate