The application of polystyrene waveguides to protein adsorption investigations.
The possibility of detecting peroxidase adsorption on the surface of a planar polystyrene waveguide has been studied using light of the wavelength adsorbed by the protein molecules. When a single-mode optical waveguide was employed, maximal protein sensitivity was obtained when the thickness of waveguide layer was about 1.5-times greater than the cutoff thickness. Adsorption kinetics of peroxidase on the surface of polystyrene was studied with single-mode waveguides. A minimum surface concentration of 0.1 mg/m2 was observed experimentally.
Silin
V I
VI
Institute of Biochemistry, Vilnius, Lithuania.
Balchytis
G A
GA
Kulys
J J
JJ
D000327:Adsorption; D006735:Horseradish Peroxidase / Q000737:chemistry*; D007700:Kinetics; D055095:Optics and Photonics; D011137:Polystyrenes / Q000737:chemistry*; D013053:Spectrophotometry; D013056:Spectrophotometry, Ultraviolet
D011137:Polystyrenes; D006735:Horseradish Peroxidase