Single injection of pentobarbital induces long-lasting effects on ANP synthesis and gene expression in the rat atria.
To define the long-term effects of pentobarbital sodium on the plasma and atrial atrial natriuretic peptide (ANP) system, experiments were performed in female Sprague-Dawley rats. The plasma levels of immunoreactive (ir) ANP showed chronic as well as acute response to pentobarbital sodium administration. A single dose (30 mg/kg, i.p.) of pentobarbital sodium resulted in a suppression in the plasma levels of irANP up to 1 week of administration. The suppressive effect on plasma irANP concentrations was dose-dependent. Right but not left atrial contents of irANP increased by an administration of pentobarbital sodium up to 4 weeks. ANP mRNA contents of the atrial exposed to pentobarbital sodium began to increase after 2 days, reached to the peak after 2 weeks, and began to return to control values after 6 weeks. Surgical stress accentuated these patterns of plasma and atrial ANP responses to pentobarbital sodium treatment. The present data, therefore, suggest that even a single anesthetic dose of pentobarbital could elicit long-lasting profound changes in ANP system, i.e., changes in gene expression, synthesis and the secretion of ANP.
Seul
K H
KH
Department of Physiology, Medical School, College of Natural Science, Jeonbug National University, Republic of Korea.
Cho
K W
KW
Kim
S H
SH
Hwang
Y H
YH
Park
C U
CU
Koh
G Y
GY
D000818:Animals; D009320:Atrial Natriuretic Factor / Q000096:biosynthesis / Q000187:drug effects* / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D006325:Heart Atria / Q000187:drug effects* / Q000378:metabolism; D007274:Injections, Intraperitoneal; D010424:Pentobarbital / Q000008:administration & dosage / Q000494:pharmacology*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley
D012333:RNA, Messenger; D009320:Atrial Natriuretic Factor; D010424:Pentobarbital