Identification of Aeromonas schubertii and Aeromonas jandaei by using a polymerase chain reaction-probe test.
Two oligonucleotide primers were used in a polymerase chain reaction-protocol to amplify a region (approx. 850 bp) of the 16S rRNA gene of Aeromonas schubertii and Aeromonas jandaei. Hybridization of the polymerase chain reaction products to specific internal probes provided a highly specific method for the identification of these two species.
Ash
C
C
Department of Microbiology, Institute of Food Research, Reading, UK.
Martinez-Murcia
A J
AJ
Collins
M D
MD
D000333:Aeromonas / Q000145:classification* / Q000235:genetics; D001483:Base Sequence; D015139:Blotting, Southern; D004275:DNA, Ribosomal / Q000235:genetics*; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D013045:Species Specificity
D004275:DNA, Ribosomal; D009838:Oligodeoxyribonucleotides; D012336:RNA, Ribosomal, 16S