Human megakaryocytes have a CD4 molecule capable of binding human immunodeficiency virus-1.
Most human megakaryocytes (MGKs) express the CD4 antigen on their surface. Approximately 25% have a CD4 receptor density comparable to that of CD4+ T cells (Basch et al, Proc Natl Acad Sci USA 87:8085, 1990). In these studies, we show: (1) the presence of mRNA for CD4 in human MGKs; (2) the binding of human immunodeficiency virus-1 (HIV-1) to human MGKs; (3) the inhibition of binding by anti-CD4 (Leu3a) antibody or rCD4; (4) the infection of a human MGK line, CHRF-288 with HIV-1; and (5) inhibition of infection with anti-CD4. Human MGKs have mRNA for CD4 as shown by in situ hybridization with an RNA probe synthesized from a 3-kb cDNA sequence of plasmid pSP65.T4.8 containing the full-length CD4 sequence. MGKs (23% +/- 17%) bound HIV-1, as determined by anti-gp120 and anti-CD41 staining. Binding to human MGKs could be inhibited 55% to 75% with anti-CD4 or rCD4, respectively. Infection of a CD4+ MGK line (CHRF-288) could be accomplished with HIV-1, as determined by proviral DNA polymerase chain reaction and p24 production. Preincubation with anti-CD4 inhibited apparent proviral DNA infection by 100% and p24 production by 65% to 70%. Thus, human MGKs have a CD4 receptor capable of binding HIV-1. Using this receptor, HIV-1 can infect cells representative of the MGK lineage.
Kouri
Y H
YH
Department of Medicine, New York University Medical Center, New York 10016.
Borkowsky
W
W
Nardi
M
M
Karpatkin
S
S
Basch
R S
RS
D015703:Antigens, CD / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D001853:Bone Marrow / Q000502:physiology*; D001854:Bone Marrow Cells; D015704:CD4 Antigens / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D004247:DNA / Q000032:analysis / Q000378:metabolism; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics; D005434:Flow Cytometry; D015683:Gene Products, gag / Q000032:analysis; D015750:Genes, gag; D015497:HIV-1 / Q000235:genetics / Q000502:physiology*; D006801:Humans; D017403:In Situ Hybridization; D008533:Megakaryocytes / Q000382:microbiology / Q000502:physiology*; D016133:Polymerase Chain Reaction; D011533:Proviruses / Q000502:physiology; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism
D015703:Antigens, CD; D015704:CD4 Antigens; D004279:DNA, Viral; D015683:Gene Products, gag; D012333:RNA, Messenger; D004247:DNA