Adhesion of Lactobacillus acidophilus to avian intestinal epithelial cells mediated by the crystalline bacterial cell surface layer (S-layer).
Lactobacillus acidophilus was isolated from washed and homogenized walls of the crop and caecum of an adult fowl. A strain that adhered well in the Fuller adhesion test was subcultured until colonies on Lactobacillus Selective agar changed from rough to smooth. This coincided with a change from aggregate to planktonic growth in liquid medium and a marked loss of ability to adhere. ultrastructure of cells from both types of culture was studied by electron microscopy. An S-layer formed the outermost part of the cell wall in the strongly-adherent strain, whereas this layer was covered with polymerized material or was absent in strains that lacked the ability to adhere, or those with reduced adherence.
Schneitz
C
C
Orion Corporation Farmos, Turku, Finland.
Nuotio
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L
Lounatma
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K
D000818:Animals; D001422:Bacterial Adhesion / Q000502:physiology*; D002432:Cecum / Q000382:microbiology*; D002462:Cell Membrane / Q000502:physiology / Q000648:ultrastructure; D002645:Chickens / Q000382:microbiology*; D003426:Crop, Avian / Q000382:microbiology*; D004848:Epithelium / Q000382:microbiology; D007779:Lactobacillus acidophilus / Q000502:physiology* / Q000648:ultrastructure; D008297:Male; D008854:Microscopy, Electron