Establishment of bone marrow-derived macrophage cell lines by soft-agar cloning: regulation of macrophage heterogeneity at bone marrow level.
To study the regulation of macrophage (m phi) heterogeneity at bone marrow level, we developed a liquid culture system in which bone marrow-derived soft-agar colonies were expanded in the presence of colony-stimulating factor-1 (CSF-1). Several cloned m phi precursor cells were established as CSF-1-dependent cell lines, and were analyzed for morphological, phenotypic and functional characteristics. The continuously proliferating cell lines expressed both immature and mature m phi markers. Only minor differences between the established cell lines were detected. Thus, our results show that during long-term culture CSF-1-responsive cloned m phi precursor cells develop along identical pathways, giving rise to progeny with comparable phenotype and function in vitro.
Wijffels
J F
JF
Department of Cell Biology, Vrije Universiteit, Amsterdam, The Netherlands.
de Rover
Z
Z
Kraal
G
G
Beelen
R H
RH
D000362:Agar; D000818:Animals; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D002999:Clone Cells; D005260:Female; D016130:Immunophenotyping; D008264:Macrophages / Q000166:cytology*; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008854:Microscopy, Electron
D000362:Agar