Analysis of testosterone esters by tandem mass spectrometry.
The electron ionization (EI) and chemical ionization (CI) mass spectra of 12 representative testosterone esters were examined to explore the various analytical options available for identification and confirmation of the esters. Using EI, a number of fragment ions indicated the identification of the testosterone moiety, but structural confirmation of the individual esters often required the observance of the molecular ion at very low relative abundance ratios. The acceptable analytical method involved CI/tandem mass spectrometry based on the production of the 2 generic product ions derived from the protonated molecule ion.
Cairns
T
T
U.S. Food and Drug Administration, Los Angeles District Laboratory, CA 90015.
Siegmund
E G
EG
Rader
B
B
D002626:Chemistry, Pharmaceutical; D004952:Esters / Q000032:analysis; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D013058:Mass Spectrometry / Q000379:methods; D013739:Testosterone / Q000032:analysis*
D004952:Esters; D013739:Testosterone