Occurrence of two nonmethylene-interrupted delta 5 polyunsaturated fatty acids in a delta 6-desaturase-defective mutant of the fungus Mortierella alpina 1S-4.
Two non-methylene-interrupted fatty acids, 5(Z),11(Z),14(Z)-eicosatrienoic acid (20:3 delta 5) and 5(Z),11(Z),14(Z),17(Z)-eicosatetraenoic acid (20: 4 delta 5), were found in a delta 6-desaturase-defective mutant of Mortierella alpina 1S-4. 20: 4 delta 5 was detected only when growth was at a temperature lower than 24 degrees C or in a culture medium supplemented with either alpha-linolenic acid or 11(Z),14(Z),17(Z)-eicosatrienoic acid. 20: 3 delta 5 was found when growth was at 12-28 degrees C. The amount of 20: 3 delta 5 was greatest (27 mg g-1 dry mycelia) when growth was at 20 degrees C, and it accounted for about 7 wt% of the total fatty acids of the mycelia. The optimum temperature for the accumulation of 20:4 delta 5 was 12 degrees C; there was 6.4 mg 20: 4 delta 5/g dry mycelia. Most of these two fatty acids (more than 75 mol%) was in the triacylglycerol fraction.
Jareonkitmongkol
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S
Department of Agricultural Chemistry, Kyoto University, Japan.
Shimizu
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Yamada
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H
D001095:Arachidonic Acids / Q000032:analysis; D044943:Fatty Acid Desaturases / Q000737:chemistry*; D005231:Fatty Acids, Unsaturated / Q000032:analysis*; D005658:Fungi / Q000235:genetics*; D050602:Linoleoyl-CoA Desaturase; D009154:Mutation; D013696:Temperature; D014280:Triglycerides / Q000737:chemistry
D001095:Arachidonic Acids; D005231:Fatty Acids, Unsaturated; D014280:Triglycerides; D044943:Fatty Acid Desaturases; D050602:Linoleoyl-CoA Desaturase