Biochemical and immunological characterization of calcium-dependent and -independent PKC isoenzymes in renal ischemia.
The PKC alpha, beta, delta, epsilon and zeta isoenzyme changes in the rat kidney cortex have been investigated after temporary occlusion of the renal artery, followed by reperfusion for up to 4 h. Increased PKC alpha, delta and epsilon immunoreactivity and PKC alpha activity occurred within 60 min of postischemic reflow, while PKC beta and zeta were unmodified. Increased PKC beta activity was present at later times of reflow (120 min), suggesting that the initial events of the regenerative repair response after an ischemic insult implicate the activation of specific PKC isoenzymes (alpha, delta, epsilon), playing a role in the control of cell proliferation.
La Porta
C A
CA
Department of General Physiology and Biochemistry, University of Milan, Italy.
Comolli
R
R
D000818:Animals; D015153:Blotting, Western; D002118:Calcium / Q000502:physiology; D007511:Ischemia / Q000201:enzymology*; D007527:Isoenzymes / Q000378:metabolism; D007672:Kidney Cortex / Q000201:enzymology*; D007674:Kidney Diseases / Q000201:enzymology*; D008297:Male; D011493:Protein Kinase C / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000201:enzymology*
D007527:Isoenzymes; D011493:Protein Kinase C; D002118:Calcium