Occurrence of a 29 kDa polysaccharide in the slime layer of both smooth and rough strains of Pseudomonas aeruginosa.
1. The lipopolysaccharide (LPS) and the extracellular products (slime) of a smooth, nonmucoid Pseudomonas aeruginosa strain (PAC IR) and its rough mutant (PAC 605) were subjected to a comparative biochemical analysis. 2. Chemical and electrophoretic analyses suggested that the slime preparation of both strains are composed mainly of similar carbohydrate components which are different from those of the respective lipopolysaccharides. 3. Chromatographic analysis of the two slime preparations on gel permeation HPLC columns revealed the presence of a major polysaccharide in both strains with an apparent molecular weight 29 kDa and a minor high molecular weight polysaccharide in the PAC IR strain.
Christofidou
M
M
Department of Microbiology, School of Medicine, University of Patras, Greece.
Karamanos
N K
NK
Mintzas
A C
AC
Dimitracopoulos
G
G
Anastassiou
E D
ED
D002241:Carbohydrates / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D005690:Galactose / Q000032:analysis; D005947:Glucose / Q000032:analysis; D008070:Lipopolysaccharides / Q000032:analysis*; D008358:Mannose / Q000032:analysis; D008970:Molecular Weight; D009154:Mutation; D011550:Pseudomonas aeruginosa / Q000737:chemistry* / Q000235:genetics; D012210:Rhamnose / Q000032:analysis
D002241:Carbohydrates; D008070:Lipopolysaccharides; D005947:Glucose; D008358:Mannose; D012210:Rhamnose; D005690:Galactose