A constant flux system using the 'magnetic cell separator' (MACS) for highly efficient T-cell depletion.
A method for highly efficient T-cell depletion using a 'Magnetic Cell Separator' (MACS) is described. T-lymphocytes were depleted from peripheral blood samples of 37 patients with chronic lymphocytic leukemia (CLL). The flow speed in the MACS column was adjusted by a syringe-driver allowing a constant flow speed of about 100 microliters/min. The mean content of T-cells after depletion was 0.17% (S.D. +/- 0.27). The effectiveness of depletion was calculated to a mean 93.7%. There was no correlation between the proportion of CD3-positive cells before and after depletion.
Kleine
H D
HD
Department of Hematology and Oncology, Hannover Medical School, FRG.
Scheinichen
D
D
Lux
E
E
Poliwoda
H
H
Freund
M
M
D017252:CD3 Complex; D002469:Cell Separation / Q000379:methods*; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000473:pathology*; D008280:Magnetics; D013601:T-Lymphocytes / Q000276:immunology / Q000473:pathology
D017252:CD3 Complex