Significance of in vitro attachment of human colon cancers to extracellular matrix proteins in experimental and clinical liver metastases.
The attachment of 7 human colon cancer lines transplantable into nude mice, and primary tumors and liver metastases from 30 patients with colon cancer to 4 extracellular matrix proteins (EMPs)--Matrigel, laminin, fibronectin, and type IV collagen--was evaluated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H (MTT) assay. Cancer cells from the 4 established tumor lines which produced experimental liver metastases in vivo showed significantly greater attachment to each EMP than those from the other 3 tumor lines which did not. Although there were no significant differences between attachment to EMPs of cancer cells from 15 clinical primary tumors with liver metastases and those without, attachment to each EMP of cells derived from liver metastases was significantly greater than that of the cells from the corresponding primary tumors in 8 cases for which liver metastases and primary tumors were examined simultaneously. Attachment to EMPs, which could be determined simply and rapidly using the MTT assay, is thus considered a significant factor in experimental and clinical liver metastases of human colon cancers.
Furukawa
T
T
Department of Surgery, School of Medicine, Keio University, Japan.
Watanabe
M
M
Kubota
T
T
Kase
S
S
Fujita
S
S
Yamamoto
T
T
Saikawa
Y
Y
Kuo
T H
TH
Tanino
H
H
Kurihara
N
N
D000818:Animals; D002448:Cell Adhesion / Q000502:physiology; D002460:Cell Line; D003110:Colonic Neoplasms / Q000378:metabolism / Q000473:pathology*; D004396:Coloring Agents; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D006801:Humans; D008113:Liver Neoplasms / Q000556:secondary*; D008297:Male; D051379:Mice; D016513:Mice, SCID; D009368:Neoplasm Transplantation; D013778:Tetrazolium Salts; D013844:Thiazoles; D014407:Tumor Cells, Cultured
D004396:Coloring Agents; D016326:Extracellular Matrix Proteins; D013778:Tetrazolium Salts; D013844:Thiazoles; C022616:thiazolyl blue