Purification and partial characterization of a bacteriocin isolated from Bacteroides ovatus H47.
A new intracellular bacteriocin isolated from a human fecal strain of Bacteroides ovatus was partially purified through ammonium sulfate precipitation, ion exchange chromatography, gel filtration, and preparative polyacrylamide gel electrophoresis (PAGE). The bacteriocin is stable at a pH range of 3-10, at 60 degrees C for 24 h, and at -70 degrees C for 6 months. It is inactivated by proteolytic enzymes. The molecular weight, estimated by sodium dodecyl sulfate--PAGE, is 78 kDa. Fifty strains of the Bacteroides fragilis group were isolated from fecal samples, and 41 of the isolates were shown to produce an antagonistic substance against at least 1 indicator strain. Iso-, auto-, and hetero-antagonisms were observed.
Miranda
C M
CM
Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
Farias
L M
LM
Carvalho
M A
MA
Damasceno
C A
CA
Totola
A H
AH
Tavares
C A
CA
Cisalpino
E O
EO
Vieira
E C
EC
D001430:Bacteriocins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D001439:Bacteroides / Q000737:chemistry* / Q000187:drug effects; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D005243:Feces / Q000382:microbiology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D013291:Streptococcus / Q000187:drug effects
D001430:Bacteriocins