Klebsiella pneumoniae infection on a rehabilitation unit: comparison of epidemiologic typing methods.
OBJECTIVE
To identify factors associated with an increased occurrence of Klebsiella pneumoniae isolation in urine cultures and infected wounds on a rehabilitation unit and to compare typing methods for K pneumoniae isolates.


METHODS
Retrospective review of laboratory reports and patient records with case-control study. Analysis of K pneumoniae isolates using capsular serotyping, enzyme electrophoretic typing, ribotyping, and DNA typing.


METHODS
48-bed rehabilitation unit in an 1,100-bed tertiary care teaching hospital in Winnipeg, Manitoba.


RESULTS
In 1988, 20 (19%) of 106 patients admitted to the rehabilitation unit had K pneumoniae isolated from urine or wound, and in 1989 31 (28%) of 111 patients had Klebsiella isolated. Review of ward practices revealed appropriate written policies but evidence of failure in execution leading to multiple opportunities for transmission among patients. Substantial environmental contamination was not identified, although a common urine graduate may have contributed to some transmission. Individuals with K pneumoniae isolated had a significantly longer duration of stay. Many of these were spinal cord-injured patients and were maintained on intermittent catheterization. One outbreak strain was identified in epidemiologic typing. Other strains were generally identified in individuals with non-nosocomial acquisition of infection. Comparison of epidemiologic typing methods suggests ribotyping may be the optimal method for typing K pneumoniae strains.


CONCLUSIONS
K pneumoniae was acquired frequently by spinal cord-injured patients with extended admissions, re-emphasizing the importance of both patients and staff following appropriate infection control practices on rehabilitation wards. Ribotyping was the optimal method for typing K pneumoniae isolates.
Thompson
W
W
Department of Medicine, University of Manitoba, Winnipeg, Canada.
Romance
L
L
Bialkowska-Hobrazanska
H
H
Rennie
R P
RP
Ashton
F
F
Nicolle
L E
LE
D000328:Adult; D000368:Aged; D015373:Bacterial Typing Techniques / Q000145:classification; D016022:Case-Control Studies; D003428:Cross Infection / Q000150:complications / Q000453:epidemiology*; D004196:Disease Outbreaks / Q000145:classification; D005260:Female; D006746:Hospital Bed Capacity, 500 and over; D006757:Hospital Units / Q000706:statistics & numerical data*; D006784:Hospitals, Teaching / Q000706:statistics & numerical data; D006801:Humans; D007710:Klebsiella Infections / Q000453:epidemiology* / Q000382:microbiology / Q000652:urine; D007711:Klebsiella pneumoniae / Q000145:classification / Q000302:isolation & purification*; D007902:Length of Stay; D008297:Male; D008875:Middle Aged; D012047:Rehabilitation Centers / Q000706:statistics & numerical data*; D012189:Retrospective Studies; D013119:Spinal Cord Injuries / Q000150:complications / Q000534:rehabilitation