[Comparison of EBL cells and ELISA in the culture and serological diagnosis of rhinitis atrophicans in swine].
Pasteurella multocida isolates from 271 nasal swabs of pigs were tested in EBL cell culture for toxin production. Mixed bacteria cultures of the same swabs were examined in the P. multocida toxin ELISA K462 (Dakopatts). In the ELISA 114 swabs reacted positive, whereas toxigenic P. multocida were detected by the EBL cell test in 86 swabs. In a neutralization test (SNT) combined with EBL cell culture and with the ELISA 111 sera were examined for P. multocida antitoxin. The toxin had to be more concentrated for the ELISA than for the cell culture; therefore the SNT with EBL cells was more sensitive. Whereas 101 sera had titres of 1:4 or higher in the cell culture, 68 of these sera were positive in the ELISA.
Alt
M
M
Tiergesundheitsamt Oldenburg der Landwirtschaftskammer Weser-Ems.
Bechmann
G
G
Schöss
P
P
D000818:Animals; D000992:Antitoxins / Q000097:blood*; D001426:Bacterial Proteins; D001427:Bacterial Toxins / Q000032:analysis* / Q000276:immunology; D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D016979:Pasteurella multocida; D012222:Rhinitis, Atrophic / Q000175:diagnosis / Q000662:veterinary*; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis*
D000992:Antitoxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; C062258:Pasteurella multocida toxin
Vergleich von EBL-Zellen und ELISA in der kulturellen und serologischen Diagnostik der Rhinitis atrophicans des Schweines.