Mapping cis-acting defects in promoters of transcriptionally silent DQA2, DQB2, and DOB genes.
Defects in promoters of the nonexpressed DQA2, DQB2, and DOB genes from the class II major histo-compatibility complex were mapped by placing Z and X boxes of these silent genes into a synthetic DRA promoter. These conserved upstream sequences confer B-cell-specific and gamma-interferon-inducible expression to the DRA gene. Since DRA promoters containing the X box from the DQA2 gene and Z boxes from DQA2, DQB2, and DOB genes were neither expressed constitutively in B cells nor inducible by gamma interferon in fibroblastic cells, these conserved upstream sequences are implicated in the transcriptional defects of these silent genes.
Voliva
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CF
Howard Hughes Medical Institute, University of California, San Francisco 94143-0724.
Tsang
S
S
Peterlin
B M
BM
D001402:B-Lymphocytes / Q000276:immunology; D001483:Base Sequence; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D005802:Genes, MHC Class II / Q000187:drug effects; D006681:HLA-D Antigens / Q000235:genetics*; D006683:HLA-DQ Antigens / Q000235:genetics*; D006367:HeLa Cells; D000949:Histocompatibility Antigens Class II; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins; D015183:Restriction Mapping; D014158:Transcription, Genetic; D014162:Transfection
C416186:H-2O antigen; D006681:HLA-D Antigens; C046148:HLA-DO antigens; D006683:HLA-DQ Antigens; C040626:HLA-Dx antigen; D000949:Histocompatibility Antigens Class II; D009838:Oligodeoxyribonucleotides; D011994:Recombinant Proteins; D007371:Interferon-gamma; D015500:Chloramphenicol O-Acetyltransferase