Tissue typing the HLA-A locus from genomic DNA by sequence-specific PCR: comparison of HLA genotype and surface expression on colorectal tumor cell lines.
A system devised for tissue typing the HLA-A locus by PCR from genomic DNA has been used to investigate abnormalities of HLA expression in a panel of 30 colorectal tumor cell lines, by comparing the HLA-A locus genotype with surface expression of HLA. Three cell lines showed complete lack of HLA expression associated with failure to express beta 2-microglobulin. In two other cell lines, loss of expression of HLA-A2 was observed, in spite of the presence of the gene in genomic DNA. Eleven cell lines gave a single HLA-A locus specificity on PCR typing. In one of these cell lines we have demonstrated the loss of an HLA-A locus gene in the tumor cell by comparison with DNA from a lymphoblastoid B-cell line derived from the same patient. These data indicate that at least three independent mechanisms were involved in the loss of HLA expression on the colorectal tumor cell lines.
Browning
M J
MJ
Imperial Cancer Research Fund, John Radcliffe Hospital, Oxford, United Kingdom.
Krausa
P
P
Rowan
A
A
Bicknell
D C
DC
Bodmer
J G
JG
Bodmer
W F
WF
D001483:Base Sequence; D003110:Colonic Neoplasms / Q000235:genetics / Q000276:immunology*; D015179:Colorectal Neoplasms / Q000235:genetics / Q000276:immunology*; D004273:DNA, Neoplasm / Q000235:genetics*; D015870:Gene Expression; D005838:Genotype; D015234:HLA-A Antigens / Q000235:genetics*; D006650:Histocompatibility Testing; D006801:Humans; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D016133:Polymerase Chain Reaction / Q000379:methods; D014407:Tumor Cells, Cultured; D001613:beta 2-Microglobulin / Q000235:genetics
D004273:DNA, Neoplasm; D015234:HLA-A Antigens; D009838:Oligodeoxyribonucleotides; D001613:beta 2-Microglobulin