Increased epithelial expression of HLA-DQ and HLA-DP molecules in salivary glands from patients with Sjögren's syndrome compared with obstructive sialadenitis.
Salivary gland specimens from 10 patients with primary Sjögren's syndrome (pSS) were examined by two-colour immunofluorescence with various combinations of monoclonal and polyclonal antibody reagents of the following specificities: human leucocyte antigen (HLA) class I and II (DR, DP and DQ), CD3, CD45 (leucocyte common antigen), various cytokeratins, and factor VIII-related antigen. Tissue specimens from 10 normal glands and 10 glands with obstructive sialadenitis (no known autoimmunity) served as controls. Only some intercalated ducts and scattered acini of the normal major glands expressed HLA class II determinants (< 5% of total epithelial area); the relative proportion of positive elements indicated differential expression (DR > DP > DQ). SS glands contained substantial T cell infiltrates and increased numbers of activated (DR+) T cells; adjacent epithelium showed extensive differential expression of HLA class II determinants (DR > DP > DQ). Glands with obstructive sialadenitis showed similarly increased epithelial expression of HLA-DR but with surprisingly small amounts of concomitant HLA-DP and -DQ expression. Epithelial HLA class II expression probably depends on cytokines as an inductive event, which is not unique for SS but particularly prominent in this disorder. Our results suggest that epithelial expression of HLA-DP or -DQ, rather than -DR, might be a prerequisite for the autoimmune process of SS to develop in genetically susceptible individuals.
Thrane
P S
PS
Laboratory for Immunohistochemistry and Immunopathology (LIIPAT), Rikshospitalet, Oslo, Norway.
Halstensen
T S
TS
Haanaes
H R
HR
Brandtzaeg
P
P
D000328:Adult; D000368:Aged; D000911:Antibodies, Monoclonal; D005260:Female; D006682:HLA-DP Antigens / Q000378:metabolism*; D006683:HLA-DQ Antigens / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D008213:Lymphocyte Activation; D008297:Male; D008875:Middle Aged; D012469:Salivary Glands / Q000276:immunology*; D012793:Sialadenitis / Q000276:immunology*; D012859:Sjogren's Syndrome / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology
D000911:Antibodies, Monoclonal; D006682:HLA-DP Antigens; D006683:HLA-DQ Antigens