title: Cytogenetic effects of promutagens in genetically engineered V79 Chinese hamster cells expressing cytochromes P450.
abstract: V79 Chinese hamster cell lines genetically engineered to express rat CYP2B1, CYP1A1, CYP1A2, and their parental cell lines V79-MZ, without acetyltransferase, and V79-NH, with acetyltransferase, were studied for chromosome aberrations and sister chromatid exchange induced by aflatoxin B1, cyclophosphamide, benzo[a]pyrene, 7,12-dimethylbenz[a]anthracene and dimethylnitrosamine. The parental V79 cell lines did not show clastogenic effects. Significant clastogenic effects were observed after an 18 h exposure to aflatoxin B1 and cyclophosphamide in CYP2B1 expressing cells, to benzo[a]pyrene in CYP1A1 and CYP1A2 expressing cells, to 7,12-dimethylbenz[a]anthracene and dimethylnitrosamine in cells, expressing CYP1A2 with or without acetyltransferase, and to cyclophosphamide in cells expressing both CYP1A2 and acetyltransferase. A significant sister chromatid exchange inducing effect was found after a 24 h exposure in each of the genetically engineered cell lines, except for benzo[a]pyrene and 7,12-dimethylbenz[a]anthracene in CYP2B1 expressing cells, and for benzo[a]pyrene in cells expressing both CYP1A2 and acetyltransferase. Thus, a battery of cell lines genetically engineered for metabolic competence may serve as a tool for investigating chromosomal changes induced by activated xenobiotics.
authors: GSF-Forschungszentrum für Umwelt und Gesundheit GmbH, Institut für Strahlenbiologie, Neuherberg, Germany.
authors: U
authors: U
authors: Kulka
authors: J
authors: J
authors: Doehmer
authors: H R
authors: HR
authors: Glatt
authors: M
authors: M
authors: Bauchinger
mesh_terms: D015127:9,10-Dimethyl-1,2-benzanthracene / Q000633:toxicity; D016604:Aflatoxin B1 / Q000633:toxicity; D000818:Animals; D001564:Benzo(a)pyrene / Q000633:toxicity; D001711:Biotransformation; D002460:Cell Line; D002869:Chromosome Aberrations / Q000235:genetics*; D006224:Cricetinae; D003412:Cricetulus; D003520:Cyclophosphamide / Q000633:toxicity; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis*; D004128:Dimethylnitrosamine / Q000633:toxicity; D005818:Genetic Engineering; D009153:Mutagens / Q000378:metabolism* / Q000633:toxicity*; D051381:Rats; D012854:Sister Chromatid Exchange / Q000187:drug effects*
chemical_list: D009153:Mutagens; D001564:Benzo(a)pyrene; D015127:9,10-Dimethyl-1,2-benzanthracene; D003520:Cyclophosphamide; D003577:Cytochrome P-450 Enzyme System; D016604:Aflatoxin B1; D004128:Dimethylnitrosamine