Retinoic acid modulation of alpha(1-->2) fucosyltransferase activity and sensitivity of tumor cells to LAK-mediated cytotoxicity.
We examined the effects of all-trans retinoic acid (RA) on alpha(1-->2) fucosyltransferase activity and sensitivity to LAK-mediated cytotoxicity in two rat colon carcinoma cell lines differing by their glycosylation state and their tumorigenic potential. RA induced a decrease in alpha(1-->2) fucosyltransferase activity in the more tumorigenic variant PROb. Fucosyltransferase mRNA levels were not affected by RA treatment in PROb cells, suggesting a posttranscriptional control. This inhibition was accompanied by a decreased expression of fucosylated membrane glycoconjugates and by a significant increase in the sensitivity to LAK-mediated cytotoxicity. REGb cells, which exhibited a very low enzymatic activity and very few fucosylated glycoconjugates, were more sensitive to LAK-lysis than PROb cells and were not affected by RA treatment.
Labarrière
N
N
Department of Medical Biochemistry, INSERM CJF-90.11, Nantes, France.
Piau
J P
JP
Zennadi
R
R
Blanchardie
P
P
Denis
M
M
Lustenberger
P
P
D000230:Adenocarcinoma; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002999:Clone Cells; D003110:Colonic Neoplasms; D003602:Cytotoxicity, Immunologic / Q000187:drug effects*; D005647:Fucosyltransferases / Q000235:genetics / Q000378:metabolism*; D015979:Killer Cells, Lymphokine-Activated / Q000276:immunology*; D007700:Kinetics; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000187:drug effects / Q000378:metabolism; D012334:RNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification; D051381:Rats; D013154:Spleen / Q000276:immunology; D014212:Tretinoin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured; D000097631:Galactoside 2-alpha-L-fucosyltransferase
D005647:Fucosyltransferases; D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D014212:Tretinoin; D000097631:Galactoside 2-alpha-L-fucosyltransferase