Effects of ultraviolet-B and PUVA on ornithine decarboxylase activity, DNA synthesis, and protein kinase C activity in mouse skin.
Ultraviolet-B and PUVA share several biological events with phorbol ester tumor promoters. The effects of ultraviolet-B irradiation and topical PUVA treatment on ornithine decarboxylase activity, DNA synthesis, and protein kinase C activity, which are known to be induced or activated by phorbol ester tumor promoter, were investigated in hairless mouse skin. Ornithine decarboxylase activity was remarkably enhanced by ultraviolet-B and PUVA. Although PUVA did not affect DNA synthesis significantly, ultraviolet-B stimulated epidermal DNA synthesis approximately 5-fold over control values at 48 h. However, unexpectedly, neither cytosolic nor membrane-bound protein kinase C activity showed any change during the 2 h after either treatment. These results suggest that the protein kinase C system is not involved in the initial signal transduction system of ultraviolet-B or PUVA, unlike the case with phorbol ester tumor promoter.
Mizuno
N
N
Department of Dermatology, Osaka City University Medical School, Japan.
Kono
T
T
Taniguchi
S
S
Fukuda
M
M
Maekawa
N
N
Hisa
T
T
Otani
S
S
Hamada
T
T
D000818:Animals; D001973:Bromodeoxyuridine; D002462:Cell Membrane / Q000201:enzymology / Q000528:radiation effects; D003600:Cytosol / Q000201:enzymology / Q000528:radiation effects; D004247:DNA / Q000096:biosynthesis / Q000528:radiation effects*; D005260:Female; D051379:Mice; D008812:Mice, Hairless; D009955:Ornithine Decarboxylase / Q000378:metabolism / Q000528:radiation effects*; D011701:PUVA Therapy; D011493:Protein Kinase C / Q000378:metabolism / Q000528:radiation effects*; D016196:S Phase / Q000528:radiation effects; D012867:Skin / Q000166:cytology / Q000201:enzymology / Q000378:metabolism / Q000528:radiation effects*; D013997:Time Factors; D014466:Ultraviolet Rays
D004247:DNA; D011493:Protein Kinase C; D009955:Ornithine Decarboxylase; D001973:Bromodeoxyuridine