title: Counterflow in isotachophoresis: computer simulation and experimental studies.
abstract: A computer model has been developed to predict the effects of co- and counter-current flows on electrophoretic transport. Model predictions are compared with experimental data obtained with the large-scale recycling isotachophoresis apparatus, recently developed in our laboratory. There were no significant changes in the steady state properties or the transient development of the Kohlrausch-adjusted profiles with and without flow. The application of counterflow in free flow isotachophoresis in early stages, before a steady state is achieved, can result in selective wash-out of slower sample components. Thus, the magnitude of counterflow can be used as a variable in optimizing separations. The characteristic features of the model render it also suitable for the simulation of electroosmotic flow in capillary electrophoresis in open tubes, where plug-type flow prevails.
authors: Center for Separation Science, University of Arizona, Tucson 85721.
authors: R R
authors: RR
authors: Deshmukh
authors: M
authors: M
authors: Bier
mesh_terms: D000838:Anions / Q000302:isolation & purification; D002412:Cations / Q000302:isolation & purification; D003198:Computer Simulation; D003377:Countercurrent Distribution; D004563:Electrochemistry; D004586:Electrophoresis / Q000295:instrumentation / Q000379:methods*; D005069:Evaluation Studies as Topic; D006801:Humans; D010949:Plasma / Q000737:chemistry
chemical_list: D000838:Anions; D002412:Cations