title: Thyroid hormone and growth hormone regulation of broiler adipocyte lipolysis.
abstract: Broiler adipocytes in culture were utilized 1) to determine acute and chronic effects of triiodothyronine (T3) and growth hormone (GH) on lipolysis; and 2) to determine whether T3 and GH act synergistically to increase lipolysis. Short-term effects of T3 and GH on lipolysis were determined by measuring glycerol release from adipocytes incubated for 1 h with T3 or GH and glucagon (GLU). To investigate long-term effects, adipocytes were cultured for 24 h with T3, GH, or a combination of T3 and GH before removal of hormones by washing and assessment of lipolysis. Basal and GLU-stimulated lipolysis were not altered by short-term (1 h) incubation of adipocytes with T3 or GH. Pretreatment of adipocytes with T3 for 24 h increased (P < .05) basal lipolysis and lipolysis in the presence of low (.3 to 1.5 ng/mL) doses of GLU. Preincubation of adipocytes with GH for 24 h decreased (P < .05) glycerol release in response to maximal stimulatory doses (3 to 10 ng/mL) of GLU and increased (P < .05) glycerol release in response to .3 ng GLU/mL. Long-term pretreatment of adipocytes with a combination of T3 and GH produced a similar increase in lipolysis with .3 ng GLU/mL as pretreatment with either T3 or GH alone. Thus, T3 and GH did not act synergistically to increase lipolysis from broiler adipocytes.
authors: Division of Animal and Veterinary Sciences, West Virginia University, Morgantown 26506-6108.
authors: R L
authors: RL
authors: Harden
authors: T P
authors: TP
authors: Oscar
mesh_terms: D000273:Adipose Tissue / Q000187:drug effects*; D000818:Animals; D002478:Cells, Cultured; D002645:Chickens / Q000378:metabolism*; D004338:Drug Combinations; D004357:Drug Synergism; D005260:Female; D005934:Glucagon / Q000494:pharmacology; D005990:Glycerol / Q000378:metabolism; D013006:Growth Hormone / Q000494:pharmacology*; D008066:Lipolysis / Q000187:drug effects*; D014284:Triiodothyronine / Q000494:pharmacology*
chemical_list: D004338:Drug Combinations; D014284:Triiodothyronine; D013006:Growth Hormone; D005934:Glucagon; D005990:Glycerol