Genetic transformation in Helicobacter pylori.
Genetic transformation in Helicobacter pylori was investigated by using its chromosomal and plasmid DNAs. Six out of the eight strains exhibited the natural competence for incorporation of H. pylori chromosomal DNA, and all the strains incorporated the donor DNA efficiently by washing and concentrating the cells with a glycerol solution. The much higher frequency of transformation was obtained in each strain by means of electroporation. Electroporation experiments were also conducted by use of the recombinant DNAs consisting of the H. pylori and Escherichia coli plasmids as the donors, and the occurrence of the homologous recombination was demonstrated between the incoming H. pylori plasmid-derived region and the corresponding region of the originally residing plasmid in H. pylori.
Tsuda
M
M
Department of Microbiology, Yamaguchi University School of Medicine, Japan.
Karita
M
M
Nakazawa
T
T
D002876:Chromosomes, Bacterial; D004269:DNA, Bacterial / Q000235:genetics; D004560:Electricity; D004926:Escherichia coli / Q000235:genetics; D005821:Genetic Techniques; D016480:Helicobacter pylori / Q000235:genetics*; D010957:Plasmids; D015183:Restriction Mapping; D014170:Transformation, Genetic
D004269:DNA, Bacterial