[Biosynthesis of bovine growth hormone in Escherichia coli DH1 cells and its purification on a pilot scale].
The biosynthesis of the bovine growth hormone (BGH) in E. coli DH1 cells containing the plasmid pbGH(1-119)ptrp was studied. It was found that BGH accumulated in the cytoplasm of bacterial cells as optically dense granules that can be isolated by low-speed centrifugation. A fraction of purified protein granules was used for further purification of BGH. A purified BGH preparation contained two polypeptides with molecular weights of 18,000 and 22,000 D. The state of plasmid was investigated during cultivation and storage of the culture.
Nikolaeva
V M
VM
Bykovskaia
S V
SV
Kozhin
V A
VA
Maĭorov
S M
SM
Usankina
T G
TG
Rukhlova
L G
LG
Luzina
I E
IE
Ezhov
V A
VA
D000818:Animals; D002417:Cattle; D004926:Escherichia coli / Q000648:ultrastructure; D013006:Growth Hormone / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification; D008854:Microscopy, Electron; D010865:Pilot Projects; D010957:Plasmids
D013006:Growth Hormone
Biosintez bych'ego gormona rosta v kletkakh Escherichia coli DH1 i ego vydelenie na pilotnom urovne.