title: Platelet-activating factor stimulates protein kinase C translocation in cerebral microvessels.
abstract: The effects of platelet-activating factor (PAF) on distribution of protein kinase C activity in brain microvessels have been examined. Our results indicate that PAF caused an increase of protein kinase C activity in membrane, accompanied by a loss of activity in the cytosol. This effect resulted to be dose-dependent and the translocation was evident after 30 min of PAF treatment. These results suggest that PAF could play a role in transport processes in the blood-brain barrier, involving protein phosphorylation by activation of protein kinase C.
authors: Departamento de Biología Molecular, Centro de Biología Molecular Severo Ochoa (CSIC-UAM), Universidad Autónoma de Madrid, Spain.
authors: R E
authors: RE
authors: Catalán
authors: A M
authors: AM
authors: Martínez
authors: M D
authors: MD
authors: Aragonés
authors: E
authors: E
authors: Garde
authors: G
authors: G
authors: Díaz
mesh_terms: D000818:Animals; D001692:Biological Transport; D001921:Brain / Q000098:blood supply* / Q000201:enzymology; D002196:Capillaries / Q000201:enzymology*; D004789:Enzyme Activation; D066298:In Vitro Techniques; D008297:Male; D010972:Platelet Activating Factor / Q000494:pharmacology*; D011493:Protein Kinase C / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D010972:Platelet Activating Factor; D011493:Protein Kinase C