Improved detection limits for screening of diuretics by coupled liquid chromatography and ultraviolet-visible spectrophotometry.
Experimental conditions have been studied in order to improve the sensitivity for the analysis of diuretics and probenecid in urine samples by high-performance liquid chromatography with ultraviolet detection. Sample clean-up and chromatographic parameters have been optimized to obtain a suitable sensitivity for the detection or quantification of each diuretic using an HP-Hypersil ODS-C18 column (5 microns, 250 mm x 4 mm I.D.), taking into account the pharmacological properties of each compound. The reliability of this method was tested by analysing urine samples after a minimum single-dose administration of chlorthalidone and probenecid.
Campíns-Falcó
P
P
Departamento de Química Analítica, Facultad de Química, Universidad de Valencia, Burjassot, Spain.
Herráez-Hernández
R
R
Sevillano-Cabeza
A
A
D002752:Chlorthalidone / Q000652:urine; D002851:Chromatography, High Pressure Liquid; D004232:Diuretics / Q000493:pharmacokinetics / Q000652:urine*; D006801:Humans; D007202:Indicators and Reagents; D011339:Probenecid / Q000652:urine; D012015:Reference Standards; D013056:Spectrophotometry, Ultraviolet
D004232:Diuretics; D007202:Indicators and Reagents; D011339:Probenecid; D002752:Chlorthalidone