title: Outer-membrane protein and immunoblot analysis of Australian isolates of Haemophilus influenzae.
abstract: Strains of Haemophilus influenzae isolated from children in South Australia and the Northern Territory with systemic infections (mostly meningitis or epiglottitis) were subjected to serotyping, biotyping, outer-membrane protein (OMP) analysis and immunoblot subtyping. All 65 isolates examined were from blood or cerebrospinal fluid; 59 (91%) of the strains were identified as type b and the remainder as either type a (two strains) or non-typable (four strains). Of the 59 type b strains, 45 (76%) belonged to a single OMP subtype (equivalent to subtype 3L in the Barenkamp scheme); the remaining type b strains belonged to five other OMP subtypes. No correlation was apparent between OMP subtype and geographical region, clinical diagnosis or antimicrobial drug susceptibility pattern. Immunoblot subtyping enabled nine (18%) of 41 strains belonging to the principal OMP subtype to be distinguished from the remainder.
authors: Department of Microbiology, Adelaide Children's Hospital Campus, North Adelaide, South Australia.
authors: D
authors: D
authors: Hansman
authors: A
authors: A
authors: Lawrence
mesh_terms: D001315:Australia; D001425:Bacterial Outer Membrane Proteins / Q000032:analysis*; D015373:Bacterial Typing Techniques; D015153:Blotting, Western; D002648:Child; D004591:Electrophoresis, Polyacrylamide Gel; D006192:Haemophilus Infections / Q000382:microbiology*; D006193:Haemophilus influenzae / Q000737:chemistry / Q000145:classification* / Q000302:isolation & purification; D006801:Humans
chemical_list: D001425:Bacterial Outer Membrane Proteins