Rapid radiochemical and chemical quality control of [11C]putrescine.
A short (4.6 x 50 mm) cation exchange column was used in conjunction with conductivity and radioactivity detectors to determine the radiochemical purity (> 99%) and specific radioactivity (0.5-1.0 Ci/mu mol) of [1-11C]putrescine prepared via Michael addition of [11C]cyanide to acrylonitrile. The absence of acrylonitrile, a rodent carcinogen, from the final preparation was verified at the 50 ng level capillary vapor-phase chromatography (VPC) using a nitrogen-phosphorus detector. Routine VPC analysis using a Poropak Q column and flame ionization detection showed that preparations contained no more than 1 microgram of acrylonitrile.
Gatley
S J
SJ
Chemistry Department, Brookhaven National Laboratory, Upton, NY 11973.
Ferrieri
R A
RA
Fowler
J S
JS
MacGregor
R R
RR
Wolf
A P
AP
D000181:Acrylonitrile / Q000302:isolation & purification*; D002250:Carbon Radioisotopes; D002273:Carcinogens / Q000302:isolation & purification*; D011700:Putrescine / Q000592:standards; D011786:Quality Control; D013997:Time Factors
D002250:Carbon Radioisotopes; D002273:Carcinogens; D000181:Acrylonitrile; D011700:Putrescine