Design of a glucose minisensor based on streptavidin-glucose oxidase complex coupling with self-assembled biotinylated phospholipid membrane on solid support.
A simple and fast procedure to prepare a glucose-sensitive minielectrode is presented. It is based on a biotin-modified phospholipid bilayer to which a streptavidin-glucose oxidase complex is coupled. The assay is based on the electrochemical detection of enzymatically generated hydrogen peroxide at the potential +670 mV. In the case of an air-saturated buffering solution the response to glucose was measured up to 50 mmol.L-1, with a linear portion up to 7 mmol.L-1. The influence of oxygen tension, pH, and temperature as well as possibly interfering substances was investigated. The prospect usage for the measurement of blood and urine was tested.
Snejdárková
M
M
Institute of Animal Biochemistry and Genetics, Slovak Academy of Sciences, Ivanka pri Dunaji.
Rehák
M
M
Otto
M
M
D001426:Bacterial Proteins / Q000737:chemistry; D015374:Biosensing Techniques; D001786:Blood Glucose / Q000032:analysis; D004800:Enzymes, Immobilized; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000378:metabolism; D006029:Glycosuria; D006801:Humans; D006861:Hydrogen Peroxide / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D008051:Lipid Bilayers; D008839:Microelectrodes; D010743:Phospholipids / Q000737:chemistry; D019809:Streptavidin; D013696:Temperature
D001426:Bacterial Proteins; D001786:Blood Glucose; D004800:Enzymes, Immobilized; D008051:Lipid Bilayers; D010743:Phospholipids; D019809:Streptavidin; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase; D005947:Glucose