title: Structure and expression of novel protein-tyrosine kinases, Emb and Emt, in hematopoietic cells.
abstract: Two novel tyrosine kinase cDNAs were obtained from murine mast cells. These kinases, Emb and Emt, constitute a novel tyrosine kinase subfamily which may also include Tec, a kinase preferentially expressed in liver, and Dsrc28, a fruit fly kinase. Both lack hydrophobic stretches characteristic of the transmembrane domains found in growth factor receptor tyrosine kinases and carboxyl-terminal, negative regulatory tyrosine residue found in Src family kinases. In addition to the Src homology region 2 (SH2) and SH3 domains characteristic of the Src family kinases and other signaling molecules, Emb and Emt share a similar amino-terminal domain comprised mainly of two repeat segments. The emb 2.7-kb transcript was expressed in mast cells, myeloid cells and B lymphocytes while the emt 4.6-kb mRNA in mast cells, myeloid cells and T lymphocytes. The evidence for in vitro tyrosine kinase activity of Emb and Emt proteins is also provided.
authors: Division of Immunobiology, La Jolla Institute for Allergy and Immunology, California 92037.
authors: N
authors: N
authors: Yamada
authors: Y
authors: Y
authors: Kawakami
authors: H
authors: H
authors: Kimura
authors: H
authors: H
authors: Fukamachi
authors: G
authors: G
authors: Baier
authors: A
authors: A
authors: Altman
authors: T
authors: T
authors: Kato
authors: Y
authors: Y
authors: Inagaki
authors: T
authors: T
authors: Kawakami
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D004277:DNA, Single-Stranded; D008407:Mast Cells / Q000201:enzymology*; D051379:Mice; D008969:Molecular Sequence Data; D010766:Phosphorylation; D011505:Protein-Tyrosine Kinases / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D014443:Tyrosine / Q000378:metabolism
chemical_list: D004277:DNA, Single-Stranded; D012333:RNA, Messenger; D014443:Tyrosine; C080812:emb protein-tyrosine kinase; D011505:Protein-Tyrosine Kinases; C080813:emt protein-tyrosine kinase