The effects of adriamycin on dental proteins formation and mineralization in vitro.
Second maxillary molars of 4-5 days old golden hamsters were exposed for 2 h in vitro to 1 mg/L adriamycin, rinsed and subsequently cultured up to 7 days without the drug. At days 3, 5 or 7 of culture the synthesis of extracellular tooth matrices and their mineralization were examined by measuring the incorporation of 3H-proline and the uptake of 45Ca and 32PO4 by the explants during a 24 h pulse labeling. Compared with unexposed control explants, exposure to adriamycin for the first 2 h of culture had no effect on total biosynthesis of proline-containing matrix proteins. However, at days 3 and 5 of culture it increased the quantity of water-soluble enamel matrix proteins (amelogenins). Adriamycin also strongly reduced the amount of organically-bound 32P-activity in a fraction extractable with guanidine-HC1-EDTA only, allegedly containing a mixture of mineral-associated proteins from both enamel and dentin. Since this decrease of 32P-activity coincided with the formation of osteodentin in the pulp as shown previously in histological and electron microscopical studies, it was speculated that osteodentin matrix may not contain the highly phosphorylated, dentin-specific phosphoproteins (DPP). Adriamycin also affected the uptake of 45Ca and 32PO4. At day 3 these values were slightly higher than control values but lower at days 5 and 7. It therefore appears that a 2 h exposure to adriamycin in concentrations as low as 1 mg/L causes an acceleration of secretory amelogenesis by tooth germs in vitro. It also induces pulp cells to form osteodentin.
Karim
A C
AC
Department of Anatomy, University of Manitoba, Winnipeg, Canada.
Bervoets
T J
TJ
Lyaruu
D M
DM
Wöltgens
J H
JH
Bronckers
A L
AL
D000818:Animals; D002118:Calcium / Q000493:pharmacokinetics*; D006224:Cricetinae; D004317:Doxorubicin / Q000494:pharmacology*; D008647:Mesocricetus; D008854:Microscopy, Electron; D008903:Minerals / Q000378:metabolism*; D010710:Phosphates / Q000493:pharmacokinetics*; D011392:Proline / Q000378:metabolism; D014176:Protein Biosynthesis; D014070:Tooth / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure
D008903:Minerals; D010710:Phosphates; D004317:Doxorubicin; D011392:Proline; D002118:Calcium