Purification of a 28 kD non-aggregating tryptic peptide of the Duffy blood group protein.
Duffy blood group antigenic epitopes are located on a 35-43 kD integral membrane protein of the erythrocyte membrane. This protein functions as a receptor for the human malaria parasite, Plasmodium vivax. The Duffy protein has been difficult to purify because of its tendency to form aggregates. Here we describe purification of a 28 kD tryptic fragment of the Duffy protein and purification of an 18 kD de-glycosylated form of the Duffy tryptic peptide using Thiopropyl Sepharose 6B chromatography and preparative SDS-PAGE. These Duffy-reactive peptides do not form aggregates and may prove amendable to protein sequencing.
Wasniowska
K
K
University of Louisville School of Medicine, KY.
Eichenberger
P
P
Kugele
F
F
Hadley
T J
TJ
D002478:Cells, Cultured; D002850:Chromatography, Gel; D004375:Duffy Blood-Group System / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D006031:Glycosylation; D006801:Humans; D010446:Peptide Fragments / Q000302:isolation & purification* / Q000378:metabolism; D014357:Trypsin
D004375:Duffy Blood-Group System; D010446:Peptide Fragments; D014357:Trypsin