Effect of ascorbic acid 2-0-alpha-glucoside on hydrocortisone-induced cataract formation in developing chick embryos: I. Comparison of the preventive effect of ascorbic acid derivatives.
The effect of ascorbic acid 2-O-alpha-glucoside (AA-2G) on hydrocortisone (HC)-induced lens opacity in developing chick embryo was examined and compared with those of ascorbic acid (AsA) and ascorbic acid 2-phosphate (AA-2P). The opacity was dose-dependently inhibited by a single administration of 10 or 20 mumol/egg of AA-2G and by three repeated administrations of 1, 3 or 10 mumol/egg of AA-2G. AA-2G was the most effective among the three compounds. Glucose did not enhance the preventive effect of AsA against HC-induced opacity, and neither dehydro ascorbic acid nor glucose also prevented HC-induced cataract. In the histological study, we observed many small vacuoles in the nuclear region of the opaque lens treated with HC. AA-2G inhibited the formation of such vacuoles, an effect closely correlated with the prevention of cataract formation.
Nagata
M
M
Central Research Laboratories, Santen Pharmaceutical Co., Ltd., Osaka, Japan.
Tanioka
H
H
Mibu
H
H
Hikida
M
M
Akiba
M
M
Yamamoto
I
I
D000818:Animals; D001205:Ascorbic Acid / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000494:pharmacology*; D002386:Cataract / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D002642:Chick Embryo; D004305:Dose-Response Relationship, Drug; D006854:Hydrocortisone; D007908:Lens, Crystalline / Q000187:drug effects* / Q000473:pathology; D014617:Vacuoles / Q000187:drug effects
C011669:ascorbate-2-phosphate; C065378:ascorbic acid 2-O-glucoside; D001205:Ascorbic Acid; D006854:Hydrocortisone