[mRNA with an extended Shine-Dalgarno sequence is translated independently of ribosomal protein S1].
The role of the ribosomal protein S1 in translation of a mRNA containing an extended Shine-Dalgarno sequence has been investigated. Using the toe printing technique, the formation of a ternary initiation complex with both S1-depleted 30S subunits and subunits treated with anti-S1 antibodies and mini-mRNA containing an lambda-cro-mRNA translational initiation region with a very long (9 nucleotides) Shine-Dalgarno sequence, has been demonstrated. It is concluded that initiation of translation on mRNA with extended SD-sequence is S1-independent. By means of an E. coli cell-free system of translation (S-100 extract), the translation of mini-cro-mRNA by S1-depleted ribosomes has been shown. In contrast with mini-cro-mRNA, the 30S subunits without protein S1 are inactive both in the ternary initiation complex formation and in cell-free translation with MS2 or fr phage RNAs and RNA protein III of phage fd.
Balakin
A G
AG
Bogdanova
S L
SL
Skripkin
E A
EA
D001483:Base Sequence; D002474:Cell-Free System; D004926:Escherichia coli; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics*; D012269:Ribosomal Proteins / Q000378:metabolism*
D012333:RNA, Messenger; D012269:Ribosomal Proteins; C015494:ribosomal protein S1
mRNK s protiazhennoĭ posledovatel'nost'iu Shaĭn-Dal'garno transliruetsia nezavisimo ot ribosomnogo belka S1.