[The point mutation of pre-C region HBV-DNA in sera from chronic hepatitis B].
To clarify the relationship between a point mutation of HBV-DNA Pre-C region and the serological feature of chronic hepatitis B, we determined the Pre-C region sequence of HBV-DNA obtained sera of 39 patients with chronic hepatitis B. The screening of the gene arrangement of Pre-C region of HBV-DNA was performed by a direct sequence method amplifying HBV-DNA by polymerase chain reaction methods. In 22 of HBeAg positive cases, a mutant type (the 28th codon changed from TGA to TAG: stop codon) was found in only one case. The other hand, In 17 of HBeAg negative cases, it was found in 6 cases with fluctuating ALT and DNA-polymerase levels. We concluded that mutant viral infections could be the main cause of HBeAg negative cases with fluctuating ALT and DNA-polymerase levels.
Miyakawa
H
H
4th Department of Internal Medicine, Teikyo University, School of Medicine.
Abe
K
K
Nakamura
H
H
Nagai
K
K
Kako
M
M
Furue
H
H
D000328:Adult; D002908:Chronic Disease; D003062:Codon; D004279:DNA, Viral / Q000235:genetics*; D005260:Female; D005814:Genes, Viral; D006509:Hepatitis B / Q000235:genetics* / Q000276:immunology / Q000517:prevention & control; D017325:Hepatitis B Vaccines; D006513:Hepatitis B e Antigens; D006515:Hepatitis B virus / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D017354:Point Mutation; D016133:Polymerase Chain Reaction
D003062:Codon; D004279:DNA, Viral; D017325:Hepatitis B Vaccines; D006513:Hepatitis B e Antigens