Synergistic effect of TEST--yolk buffer treatment and glass wool filtration of spermatozoa on the outcome of the hamster oocyte penetration assay.
Enhanced penetration of zona-free hamster oocytes occurs after spermatozoa have either been treated with TEST--yolk buffer or processed by glass wool filtration. The present study was conducted to determine if a synergistic effect on fertilizing ability could be achieved by combining these two treatments. Ejaculates (n = 16) were treated with TEST--yolk buffer (TYB) and divided into two aliquots. One aliquot was kept as control and the other aliquot was filtered through glass wool prior to the sperm penetration assay. The TYB--glass wool filtered spermatozoa penetrated a significantly higher percentage of oocytes than the TYB treated spermatozoa.
Holmgren
W J
WJ
Department of Obstetrics and Gynecology, Northwestern University Medical School, Prentice Women's Hospital, Chicago, IL 60611.
Jeyendran
R S
RS
D000818:Animals; D002021:Buffers; D002469:Cell Separation; D006224:Cricetinae; D005260:Female; D005307:Fertilization in Vitro; D005374:Filtration; D005898:Glass; D005947:Glucose; D006801:Humans; D007248:Infertility, Male / Q000503:physiopathology*; D008297:Male; D013084:Sperm-Ovum Interactions; D013094:Spermatozoa / Q000502:physiology*; D014325:Tromethamine
D002021:Buffers; C077847:TES-Tris yolk buffer; D014325:Tromethamine; D005947:Glucose