Human lymphocyte-specific pp52 gene is a member of a highly conserved dispersed family.
For a better understanding of genes that potentially function in B lymphocyte cell signaling, we isolated the human genomic counterpart of the murine pp52 or LSP1 gene. We unexpectedly found that the human pp52 gene is one of four closely related loci. Representative cosmids from each of the four family members were isolated and chromosomally localized by fluorescence in situ hybridization. Nucleotide sequence was obtained from an exon common to each locus and demonstrated very close similarity among all four loci. Two of the four loci harbored dysfunctional frameshift mutations or premature translation stop sites. The exon of one locus was flanked by an 80-bp perfect inverted repeat, suggesting that it may have originated through a looped intermediate DNA structure. Through a series of cDNA hybridization studies and nucleotide sequence analyses we were able to unambiguously link the lymphocyte-expressed gene to the locus mapped to chromosome 11p15.5. This same chromosomal band has been involved in tumor-related chromosomal translocations found in chronic lymphocytic leukemia.
May
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Department of Pediatrics, Gwynne Hazen Cherry Memorial Laboratories, Cedars-Sinai Medical Center, University of California, Los Angeles 90024.
Korenberg
J R
JR
Chen
X N
XN
Lunsford
L
L
Wood
W J
WJ
Thompson
A
A
Wall
R
R
Denny
C T
CT
D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology / Q000378:metabolism*; D001483:Base Sequence; D002135:Calcium-Binding Proteins / Q000235:genetics*; D002880:Chromosomes, Human, Pair 11; D017124:Conserved Sequence; D003360:Cosmids; D004247:DNA; D015342:DNA Probes; D015723:Gene Library; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D008840:Microfilament Proteins; D008969:Molecular Sequence Data; D005810:Multigene Family; D009693:Nucleic Acid Hybridization; D015183:Restriction Mapping; D014407:Tumor Cells, Cultured
D002135:Calcium-Binding Proteins; D015342:DNA Probes; C489183:LSP1 protein, human; C057701:Lsp1 protein, mouse; D008840:Microfilament Proteins; D004247:DNA