[Experimental studies on the development of an antigen-capture test (Capture-ELIFA) for the detection of Coxiella burnetii in cow's milk as a possible alternative to animal studies].
A sensitive and specific Capture-ELIFA (Enzyme-linked-Immunofluorescent Assay) for the detection of Coxiella burnetii was developed using a monoclonal antibody and a rabbit immune serum, both directed against Coxiella burnetii, strain Nine Mile, phase I. A combination of centrifugation, Pronase-K-digestion and heat treatment proved to be advantageous for concentrating, exposure and inactivating of Coxiella antigens in milk samples. Using this method of sample preparation 4 x 10(3) coxiella particles/ml could be detected in experimentally contaminated milk samples by the optimized Capture-ELIFA. Preliminary results of comparative investigations indicate that the Capture-ELIFA is more sensitive than the inoculation of guinea pigs and subsequent detection of seroconversion.
Dackau
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D000826:Animal Testing Alternatives; D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis*; D016997:Coxiella burnetii / Q000302:isolation & purification*; D005260:Female; D005455:Fluorescent Antibody Technique; D006168:Guinea Pigs; D008892:Milk / Q000382:microbiology*; D011778:Q Fever / Q000175:diagnosis / Q000662:veterinary*; D012680:Sensitivity and Specificity
Experimentelle Untersuchungen zur Entwicklung eines Antigen-Fangtestes (Capture-Elifa) zum Nachweis von Coxiella burnetii in Kuhmilch als mögliche Alternative zum Tierversuch.