title: A comparative study of the solution structures of tachyplesin I and a novel anti-HIV synthetic peptide, T22 ([Tyr5,12, Lys7]-polyphemusin II), determined by nuclear magnetic resonance.
abstract: The solution structure of tachyplesin I, which was isolated from membrane acid extracts of the hemocytes from the Japanese horseshoe crab (Tachypleus tridentatus), was determined by nuclear magnetic resonance (NMR) and distance geometry calculation. Tachyplesin I takes an antiparallel beta-sheet structure with a type-II beta-turn. Recently, among more than 20 synthetic peptides associated with tachyplesin and its isopeptide (polyphemusin), we found that a novel compound, which we designated as T22 ([Tyr5,12, Lys7]-polyphemusin II), strongly inhibited the human immunodeficiency virus (HIV)-1-induced cytopathic effect and viral antigen expression. The solution structure of T22 was investigated using NMR, and its secondary structure was confirmed to be similar to that of tachyplesin I. The anti-parallel beta-sheet structure and the several amino-acid side chains on the plane of the beta-sheet of T22 are thought to be associated with the expression of anti-HIV activity.
authors: Faculty of Pharmaceutical Sciences, Kyoto University, Japan.
authors: H
authors: H
authors: Tamamura
authors: M
authors: M
authors: Kuroda
authors: M
authors: M
authors: Masuda
authors: A
authors: A
authors: Otaka
authors: S
authors: S
authors: Funakoshi
authors: H
authors: H
authors: Nakashima
authors: N
authors: N
authors: Yamamoto
authors: M
authors: M
authors: Waki
authors: A
authors: A
authors: Matsumoto
authors: J M
authors: JM
authors: Lancelin
mesh_terms: D000595:Amino Acid Sequence; D023181:Antimicrobial Cationic Peptides; D000998:Antiviral Agents / Q000737:chemistry*; D004268:DNA-Binding Proteins / Q000737:chemistry*; D015497:HIV-1 / Q000187:drug effects*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008958:Models, Molecular; D008969:Molecular Sequence Data; D015394:Molecular Structure; D010455:Peptides / Q000737:chemistry*; D010456:Peptides, Cyclic; D011487:Protein Conformation; D012996:Solutions; D013329:Structure-Activity Relationship
chemical_list: D023181:Antimicrobial Cationic Peptides; D000998:Antiviral Agents; D004268:DNA-Binding Proteins; D010455:Peptides; D010456:Peptides, Cyclic; D012996:Solutions; C057636:tachyplesin peptide, Tachypleus tridentatus; C062385:polyphemusin I; C076963:T22 protein, synthetic