Drug monitoring of clomipramine and desmethylclomipramine in depressed patients using a new liquid chromatographic assay.
A method has been developed for the quantitative analysis of clomipramine and its major metabolite desmethylclomipramine in plasma, using normal phase chromatography with UV detection at 254 nm. This rapid (6 min) and highly sensitive methodology (detection limits 0.5 ng/mL and 2 ng/mL for clomipramine and desmethylclomipramine, respectively; S/N = 3, 0.001 aufs) allows pharmacokinetic studies and drug monitoring of the two compounds. Using the described methodology we report an application which involved 10 depressed inpatients.
Diquet
B
B
Département de Pharmacologie Clinique, Groupe Hospitalier Pitié-Salpêtrière, Paris, France.
Thomaré
P
P
Bocquentin
M
M
Diviné
C
C
D000368:Aged; D000369:Aged, 80 and over; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002997:Clomipramine / Q000031:analogs & derivatives* / Q000097:blood*; D003863:Depression / Q000097:blood*; D016903:Drug Monitoring / Q000379:methods*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet
C012273:desmethylclomipramine; D002997:Clomipramine