Schistosoma mansoni: membranes from adult worms reversibly perturb shape, volume, and membrane organization of intact human red blood cells.
Adult forms of Schistosoma mansoni ingest host (human) red blood cells (RBCs). To elucidate potential mechanisms by which contact with adult parasites perturbs RBC membranes, we studied the effects of the membrane fraction of isolated schistosomes on RBC shape, volume, potassium ion content, and phospholipid and transmembrane protein lateral mobility. S. mansoni-treated RBCs exhibited rapid but spontaneously reversible shape change from discocytes to spheroechinocytes, reversible decrease in cell volume, and rapid loss of intracellular potassium ions. Treated RBCs also showed rapid but spontaneously reversible immobilization of membrane phospholipids and of band 3, the major transmembrane protein. These data suggest that components of adult S. mansoni membranes can perturb host RBC volume and membrane organization. In the absence of RBC lysis, RBC metabolic and repair mechanisms can reverse these effects.
Thatte
H S
HS
Department of Medicine, Harvard Medical School, Boston, Massachusetts.
Kasschau
M R
MR
Furlong
S T
ST
Byam-Smith
M P
MP
Williams
D F
DF
Golan
D E
DE
D000818:Animals; D001457:Anion Exchange Protein 1, Erythrocyte; D001692:Biological Transport; D048429:Cell Size; D006224:Cricetinae; D004912:Erythrocytes / Q000473:pathology*; D006801:Humans; D008560:Membrane Fluidity; D008566:Membranes / Q000502:physiology*; D008647:Mesocricetus; D010743:Phospholipids; D011188:Potassium / Q000378:metabolism; D012550:Schistosoma mansoni / Q000502:physiology*
D001457:Anion Exchange Protein 1, Erythrocyte; D010743:Phospholipids; D011188:Potassium