Impairment of V(D)J recombination in double-strand break repair mutants.
Cells maintain the integrity of their genome through an intricate network of repair systems that recognize and remove lesions from DNA. The only known site-directed recombination process in vertebrates is the V(D)J recombination of lymphocyte antigen receptor genes. A large panel of cell lines deficient in DNA repair were tested for the ability to perform V(D)J recombination after introduction of the RAG-1 and RAG-2 genes. Two mutants failed to generate normal V(D)J recombination, and further analysis provided evidence for two distinct nonlymphoid-specific genes that encode factors involved in both DNA repair and V(D)J recombination.
Taccioli
G E
GE
Howard Hughes Medical Institute, Children's Hospital, Boston, MA.
Rathbun
G
G
Oltz
E
E
Stamato
T
T
Jeggo
P A
PA
Alt
F W
FW
D000818:Animals; D001483:Base Sequence; D016466:CHO Cells; D002460:Cell Line; D006224:Cricetinae; D004254:DNA Nucleotidyltransferases / Q000235:genetics / Q000378:metabolism; D004260:DNA Repair; D015329:Gene Rearrangement, T-Lymphocyte; D016654:Genes, RAG-1; D007143:Immunoglobulin Heavy Chains; D008969:Molecular Sequence Data; D009154:Mutation; D011948:Receptors, Antigen, T-Cell / Q000235:genetics*; D011995:Recombination, Genetic; D045567:VDJ Recombinases
D007143:Immunoglobulin Heavy Chains; D011948:Receptors, Antigen, T-Cell; D004254:DNA Nucleotidyltransferases; D045567:VDJ Recombinases