Purification and properties of 2'-hydroxybenzalpyruvate aldolase from a bacterium that degrades naphthalenesulfonates.
2'-Hydroxybenzalpyruvate aldolase catalyzes the cleavage of 2'-hydroxybenzalpyruvate to salicylaldehyde and pyruvate. This reaction is part of the degradative pathways for naphthalene and naphthalenesulfonates by bacteria. 2'-Hydroxybenzalpyruvate aldolase has been purified to homogeneity from a bacterium that degrades naphthalenesulfonates (strain BN6). The enzyme has a molecular weight of about 120,000 and is composed of identical subunits with a molecular weight of about 38,500. Thus the enzyme appears to exist as a trimeric oligomer. The NH2-terminal amino acid sequence did not show significant homology to other published amino acid sequences. Extensive loss of enzyme activity occurred when the enzyme was incubated with 2'-hydroxybenzalpyruvate in the presence of sodium borhydride. This suggested the intermediate formation of a stable Schiff base between enzyme and substrate. 2'-Hydroxybenzalpyruvate aldolase was inhibited by p-chloromercuribenzoate and by the reaction product salicylaldehyde. The enzyme converted 2'-hydroxybenzalpyruvate, 2',4'- and 2',6'-dihydroxybenzalpyruvate.
Kuhm
A E
AE
Institut für Mikrobiologie, Universität Stuttgart, Germany.
Knackmuss
H J
HJ
Stolz
A
A
D000446:Aldehyde-Lyases / Q000037:antagonists & inhibitors / Q000302:isolation & purification* / Q000378:metabolism*; D000447:Aldehydes / Q000494:pharmacology; D000595:Amino Acid Sequence; D001711:Biotransformation; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D007700:Kinetics; D008969:Molecular Sequence Data; D009281:Naphthalenes / Q000378:metabolism; D009282:Naphthalenesulfonates / Q000378:metabolism*; D011549:Pseudomonas / Q000201:enzymology*; D013053:Spectrophotometry; D013379:Substrate Specificity
D000447:Aldehydes; D009281:Naphthalenes; D009282:Naphthalenesulfonates; C013243:salicylaldehyde; C031721:naphthalene; C024247:2'-hydroxybenzalpyruvate aldolase; D000446:Aldehyde-Lyases