Expression of bacteriophage PhiX174 lysis gene E in Staphylococcus carnosus TM300.
Expression of the cloned PhiX174 gene E causes lysis of the Gram-negative bacterium Escherichia coli, which led to the proposal that a two-membrane system is necessary for the protein E lysis function. Gene E was cloned in an E. coli/Bacillus subtilis shuttle vector and expressed in the Gram-positive bacterium Staphylococcus carnosus TM300. Regulated gene E expression had a lethal effect on S. carnosus; however, no lysis was detected, lending support to the hypothesis.
Halfmann
G
G
Institut für Mikrobiologie und Genetik, Universität Wien, Vienna, Austria.
Götz
F
F
Lubitz
W
W
D010584:Bacteriophage phi X 174 / Q000235:genetics*; D001483:Base Sequence; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development; D005814:Genes, Viral / Q000235:genetics*; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics; D013210:Staphylococcus / Q000235:genetics / Q000254:growth & development; D014764:Viral Proteins / Q000096:biosynthesis / Q000235:genetics*
C029555:E protein, bacteriophage X174; D014764:Viral Proteins