Expression of gonococcal transferrin-binding protein 1 causes Escherichia coli to bind human transferrin.
The gene for gonococcal transferrin-binding protein 1 (TBP1) was cloned behind an inducible promoter in Escherichia coli. The resultant strain was capable of binding human transferrin with the same specificity as that of the gonococcus. E. coli expressing TBP1 did not internalize transferrin-bound iron or grow on transferrin as a sole iron source.
Cornelissen
C N
CN
Department of Medicine, School of Medicine, University of North Carolina, Chapel Hill 27599.
Biswas
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GD
Sparling
P F
PF
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics*; D002352:Carrier Proteins / Q000235:genetics* / Q000502:physiology; D002417:Cattle; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D006736:Horses; D006801:Humans; D033862:Iron-Binding Proteins; D051379:Mice; D009344:Neisseria gonorrhoeae / Q000235:genetics*; D051381:Rats; D012756:Sheep; D013045:Species Specificity; D014168:Transferrin / Q000378:metabolism*; D033863:Transferrin-Binding Proteins
D001426:Bacterial Proteins; D002352:Carrier Proteins; D033862:Iron-Binding Proteins; D014168:Transferrin; D033863:Transferrin-Binding Proteins