Effects of growth factors on cell cycle arrest in dolichyl phosphate-depleted cultures.
Previously we showed that CHO cell growth is arrested in the G1 or G0 phase within 24 h after the biosynthesis of mevalonic acid is blocked. The growth-limiting factor under these conditions appeared to be dolichyl phosphate or one of its glycosylated derivatives with consequent decrease in the synthesis of N-linked glycoproteins (Doyle, J.W., and A.A. Kandutsch, 1988, J. Cell Physiol. 137:133-140; Kabakoff, B., J.W. Doyle, and A.A. Kandutsch, 1990, Arch. Biochem. Biophys. 276:382-389). We show herein that cell surface glycoproteins are depleted in the inhibited cultures and that growth arrest is delayed when supraphysiological concentrations of insulin, insulin-like growth factor-1 (IGF-1) and bFGF are added to the culture medium. Apparently an elevated level of a growth factor increases the length of time during which a threshold level of occupied receptor is maintained as the number of glycosylated receptor molecules declines. The results support the idea that cellular levels of dolichyl phosphate and its derivatives may limit cell division by controlling the numbers of functional receptors for growth factors and of other glycoproteins on the cell surface.
Doyle
J W
JW
Jackson Laboratory, Bar Harbor, Maine 04609-0800.
Ward-Bailey
P F
PF
Kandutsch
A A
AA
D000818:Animals; D001798:Blood Proteins / Q000494:pharmacology; D016466:CHO Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D002417:Cattle / Q000097:blood; D002453:Cell Cycle / Q000187:drug effects; D002478:Cells, Cultured; D006224:Cricetinae; D004247:DNA / Q000096:biosynthesis; D004288:Dolichol Phosphates / Q000378:metabolism*; D005346:Fibroblast Growth Factors / Q000494:pharmacology; D006133:Growth Substances / Q000494:pharmacology*; D008798:Mevalonic Acid / Q000378:metabolism / Q000494:pharmacology; D014415:Tunicamycin / Q000031:analogs & derivatives / Q000494:pharmacology
D001798:Blood Proteins; D004288:Dolichol Phosphates; D006133:Growth Substances; D014415:Tunicamycin; C028306:dolichol monophosphate; D005346:Fibroblast Growth Factors; C046159:tunicamycin B2; D004247:DNA; D008798:Mevalonic Acid