Identification of Streptococcus mutans antigen D as the HPr component of the sugar-phosphotransferase transport system.
Extraction of Streptococcus mutans with the detergents HECAMEG and lauroyl sarcosinate preferentially extracted antigen D, previously identified as a low molecular mass wall-associated protein. Western blotting with specific antisera was used to demonstrate that this antigen is the HPr component of the sugar-phosphotransferase transport system. Non-denaturing electrophoresis indicated that HECAMEG selectively extracted only one of the two forms of HPr. It is suggested that this form of HPr may have a specific cell surface location.
Sutcliffe
I C
IC
Department of Oral Biology, Dental School, University of Newcastle upon Tyne, UK.
Hogg
S D
SD
Russell
R R
RR
D000942:Antigens, Bacterial / Q000302:isolation & purification*; D001426:Bacterial Proteins / Q000276:immunology / Q000302:isolation & purification*; D015153:Blotting, Western; D003902:Detergents; D004591:Electrophoresis, Polyacrylamide Gel; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000276:immunology / Q000302:isolation & purification*; D013295:Streptococcus mutans / Q000737:chemistry / Q000276:immunology*
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D003902:Detergents; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System; C030182:phosphocarrier protein HPr