The binding of a complex cobalt ruthenium polyamine by deoxyribonucleic acid and a lipopolysaccharide: a model for a novel class of drugs.
In this paper we discuss the following: 1. Synthesis of [Co(H3CsarNHCH2pyRu(NH3)5)] (PF6)5, (CoRu). 2. Interaction of CoRu with calf thymus DNA and with lipopolysaccharide from Escherichia coli C (LPS) has been estimated using the absorption of the complex at 242 and 420 nm. 3. DNA and LPS increase the rate of fall of absorption at 420 nm due to autooxidation of the complex. 4. The fall in absorption of CoRu(II) at 420 nm can be used to give an approximate measure of binding to DNA and to LPS. 5. Both macromolecules are aggregated by CoRu at high concentrations and the cation and macromolecule complex can be removed by low speed centrifugation. 6. The DNA-CoRu complex can also be removed by high speed centrifugation when the cation concentration is too low to cause aggregation (20 microM CoRu/155 microM DNA-P). Absorption of redissolved complex at 420 nm is restored by reduction with ascorbic acid. 7. At saturation the ratio of mole CoRu bound/mole DNA-P is 0.16.
Hammershøi
A
A
Department of Chemistry, H. C. Orsted Institute, University of Copenhagen, Denmark.
Nord
G
G
Rowatt
E
E
Skov
L K
LK
Williams
R J
RJ
D001205:Ascorbic Acid / Q000494:pharmacology; D003035:Cobalt / Q000737:chemistry / Q000378:metabolism*; D004247:DNA / Q000378:metabolism*; D004563:Electrochemistry; D004926:Escherichia coli; D008027:Light; D008070:Lipopolysaccharides / Q000378:metabolism*; D015394:Molecular Structure; D011073:Polyamines / Q000737:chemistry / Q000378:metabolism*; D012428:Ruthenium / Q000737:chemistry / Q000378:metabolism*; D013053:Spectrophotometry
D008070:Lipopolysaccharides; D011073:Polyamines; D003035:Cobalt; D012428:Ruthenium; D004247:DNA; D001205:Ascorbic Acid