title: Physical mapping and YAC contig analysis of the region surrounding Xist on the mouse X chromosome.
abstract: The Xist sequence has been proposed as a potential candidate for the X-inactivation center based both on its localization within the candidate region for the X-inactivation center in man and mouse and on its unique pattern of expression from the inactive X chromosome. We have cloned 550 kb of DNA surrounding the mouse Xist sequence in contiguously overlapping YAC clones and have developed a long-range restriction map that spans almost 1 Mb of this region and includes this YAC contig. The detailed restriction map we have established provides a framework for the identification of expressed sequences other than Xist that may equally exhibit unusual expression characteristics associated with X inactivation. The presence of possible structural or methylation differences within this region between the active and inactive X chromosomes has been investigated through comparative analysis of male and female genomic DNA, and we report here the identification of certain CpG-containing restriction sites around Xist that have an interesting differential methylation status on the inactive and active X chromosomes.
authors: Unité de Génétique Moléculaire Murine, Institut Pasteur, Paris, France.
authors: E
authors: E
authors: Heard
authors: M C
authors: MC
authors: Simmler
authors: Z
authors: Z
authors: Larin
authors: C
authors: C
authors: Rougeulle
authors: B
authors: B
authors: Courtier
authors: H
authors: H
authors: Lehrach
authors: P
authors: P
authors: Avner
mesh_terms: D000818:Animals; D015825:Chromosomes, Fungal; D004247:DNA; D015226:Dinucleoside Phosphates / Q000378:metabolism; D004303:Dosage Compensation, Genetic; D005260:Female; D017353:Gene Deletion; D015321:Gene Rearrangement; D015698:Genomic Library; D006801:Humans; D006822:Hybrid Cells; D008297:Male; D008745:Methylation; D051379:Mice; D015183:Restriction Mapping; D014960:X Chromosome
chemical_list: D004247:DNA; D015226:Dinucleoside Phosphates; C015772:CpG dinucleotide