Regulation of c-fos proto-oncogene expression by growth hormone: protein synthesis is not required for down-regulation.
Growth hormone (GH) has been previously shown in Ob1771 adipose cells to transiently stimulate the expression of the c-fos gene by a protein kinase C (PKC)-dependent pathway. This regulation takes place at a transcriptional level. In the presence of cycloheximide (CHX), stimulation by PKC activators or by serum leads to a "superinduction" of the c-fos gene. In contrast, upon GH stimulation in the presence of CHX, no superinduction takes place and neither prolonged transcription nor mRNA stabilization are observed.
Barcellini-Couget
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S
Centre de Biochimie (UMR 134 CNRS), Université de Nice-Sophia Antipolis, UFR Sciences, France.
Doglio
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A
Dani
C
C
D000818:Animals; D002460:Cell Line; D003513:Cycloheximide / Q000494:pharmacology; D005786:Gene Expression Regulation; D016762:Genes, fos; D013006:Growth Hormone / Q000494:pharmacology / Q000502:physiology*; D006207:Half-Life; D014176:Protein Biosynthesis; D011506:Proteins / Q000037:antagonists & inhibitors; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D014158:Transcription, Genetic / Q000187:drug effects
D011506:Proteins; D016760:Proto-Oncogene Proteins c-fos; D012333:RNA, Messenger; D013006:Growth Hormone; D003513:Cycloheximide