Spatial analysis of intranuclear human repetitive DNA regions by in situ hybridization and digital fluorescence microscopy.
Non-isotopic (fluorescent) in situ hybridization has established itself as a useful technique for the localization of DNA sequences in both metaphase and interphase cells. The rapid development of digital fluorescence microscopy, especially confocal microscopy, has become a powerful aid for the evaluation of the hybridization results in cytogenetic and cell biological applications. In this review we will demonstrate the utility of these methodologies for the three-dimensional visualization and analysis of chromosome-specific (peri)centromeric repetitive DNA sequences within the intranuclear structure of human cells and cell lines.
van Dekken
H
H
Department of Pathology, Erasmus University Rotterdam, The Netherlands.
Hulspas
R
R
D002467:Cell Nucleus / Q000737:chemistry; D004247:DNA / Q000032:analysis*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D017404:In Situ Hybridization, Fluorescence; D012091:Repetitive Sequences, Nucleic Acid
D004247:DNA