title: Increasing the chemical potential of the germ-line antibody repertoire.
abstract: To augment the chemical potential of the immunological repertoire, a metal ion-binding light chain has been introduced into the murine genome. Mice containing the transgene were subsequently immunized with a fluorescein conjugate. The transgenic light chain was found at a high frequency in the anti-fluorescein memory B-cell compartment. This general method should be applicable to other cofactors and small molecules and should lead to generation of antibodies with unique catalytic activities.
authors: Department of Neuropharmacology, Scripps Research Institute, La Jolla, CA 92037.
authors: N
authors: N
authors: Sarvetnick
authors: D
authors: D
authors: Gurushanthaiah
authors: N
authors: N
authors: Han
authors: J
authors: J
authors: Prudent
authors: P
authors: P
authors: Schultz
authors: R
authors: R
authors: Lerner
mesh_terms: D000818:Animals; D000917:Antibody Formation; D001402:B-Lymphocytes / Q000276:immunology*; D001483:Base Sequence; D003433:Crosses, Genetic; D005091:Exons; D006825:Hybridomas / Q000276:immunology; D007145:Immunoglobulin kappa-Chains / Q000235:genetics* / Q000378:metabolism*; D008099:Liver / Q000276:immunology; D051379:Mice; D008815:Mice, Inbred Strains; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009838:Oligodeoxyribonucleotides; D016133:Polymerase Chain Reaction / Q000379:methods; D015183:Restriction Mapping; D013154:Spleen / Q000276:immunology; D014407:Tumor Cells, Cultured
chemical_list: D007145:Immunoglobulin kappa-Chains; D009838:Oligodeoxyribonucleotides