Biochemical and immunological properties of two forms of pertactin, the 69,000-molecular-weight outer membrane protein of Bordetella pertussis.
Two apparent isoforms of the virulence-associated 69,000-molecular-weight protein pertactin were purified from Bordetella pertussis. Mass spectrometry showed a difference of 2,060 Da, which may result from differential C-terminal cleavage of a larger precursor. Both forms were protective in a mouse model, eliciting bactericidal antibodies and reducing respiratory tract colonization.
Gotto
J W
JW
Lederle-Praxis Biologicals, American Cyanamid Company, Pearl River, New York 10965.
Eckhardt
T
T
Reilly
P A
PA
Scott
J V
JV
Cowell
J L
JL
Metcalf
T N
TN
Mountzouros
K
K
Gibbons
J J
JJ
Siegel
M
M
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000942:Antigens, Bacterial / Q000737:chemistry; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry* / Q000276:immunology; D001428:Bacterial Vaccines / Q000276:immunology; D015153:Blotting, Western; D001886:Bordetella pertussis / Q000737:chemistry* / Q000276:immunology; D007114:Immunization; D013058:Mass Spectrometry; D051379:Mice; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010566:Virulence Factors, Bordetella
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines; D010566:Virulence Factors, Bordetella; C068929:pertactin