Apolipoprotein E: binding to soluble Alzheimer's beta-amyloid.
Apolipoprotein E (apo E) is associated with Alzheimer's beta-amyloid (A beta) in senile plaques. A beta is now known to be a normal soluble peptide (sA beta) found in the cerebrospinal fluid (CSF) and other biological fluids. We have used synthetic A beta peptides bound to affinity membranes in order to determine whether apo E or any other amyloid associated protein will bind to these membranes, when they are bathed in CSF. Under these conditions apo E, as well as another apolipoprotein, apolipoprotein J (Apo J), bound to the membranes. Using ELISA and ligand binding studies, we found a high avidity binding of A beta peptides to apo E. This suggests that apo E, as well as other related proteins may bind not only amyloid A beta but also sA beta. This interaction may be critical in amyloid formation.
Wisniewski
T
T
Department of Pathology, NY Univ Medical Center, NY 10016.
Golabek
A
A
Matsubara
E
E
Ghiso
J
J
Frangione
B
B
D000544:Alzheimer Disease / Q000378:metabolism*; D016229:Amyloid beta-Peptides / Q000378:metabolism*; D001057:Apolipoproteins E / Q000378:metabolism*; D001665:Binding Sites; D015153:Blotting, Western; D002555:Cerebrospinal Fluid; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D016622:Silver Staining
D016229:Amyloid beta-Peptides; D001057:Apolipoproteins E