Laser-light induced chromosome aberrations in Chinese hamster cells.
Wild-type Chinese hamster cells CHO K1 and their radiosensitive mutant xrs5 were irradiated at 308 nm, using light pulses of a XeCl excimer laser with total energy fluences of 0.1 kJ/m2 to 4.08 kJ/m2. Chromosome-type and chromatid-type chromosome aberrations have been observed at pulse irradiances of 2.5 x 10(7) W/m2 and 1.7 x 10(8) W/m2, indicating that in mammalian cells DNA double-strand breaks occur already in this irradiance range. The results obtained with laser irradiation are compared with X-ray irradiation.
Virsik-Peuckert
P
P
Institute of Medical Physics and Biophysics, University of Göttingen, Germany.
Hillrichs
G
G
Neu
W
W
Harder
D
D
D000818:Animals; D016466:CHO Cells; D002869:Chromosome Aberrations; D002875:Chromosomes / Q000528:radiation effects*; D006224:Cricetinae; D004307:Dose-Response Relationship, Radiation; D007834:Lasers; D008027:Light