[Changes in the structure of the respiratory chain of the mitochondria from different tissues in relation to the conditions of mammalian development].
The content and stoichiometric relations of respiratory chain cytochromes were studied in mitochondria from femoral muscles of Wistar rats developing either under the conditions of hypokinesia or in near-natural ones. It was shown that, during early postnatal ontogenesis (until puberty), the maximum level of cytochrome aa3 was achieved already within the playing period (by the 30th day) in animals developing under near-natural conditions while in the group with locomotory deprivation, it was approached only in the midst of puberty (by the 45th day). By the age of sexual maturity (the 60th day), the oxidative potential was higher in the group with natural development. Stoichiometric changes occurred in the relation of muscular cytochromes within the considered period. The cytochrome b562/aa3 ratio in 30-day-old rat pups from the group with "normal" development was twice as high as in adults. Similar alteration of this ratio was observed also in the group of animals developing under the conditions of hypokinesia though 5 days later. The interorganic analysis showed that the cytochrome ratio similar to that observed in muscles of rat pups within the playing period was characteristic of the liver and similar to that of adults-of the brain. Functional peculiarities of mitochondrial activity was studied on mitochondria isolated from brain and liver of adult animals. It was shown that the difference in respiratory chain structure, mainly in the b562/aa3 ratio, was connected with different substrate specificity. Brain mitochondria characterized with the b562/aa3 ratio below 1 possessed a higher affinity to NADH-dependent substrates while hepatic mitochondria with the ratio above 1-to FADH-dependent substrates.
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D000818:Animals; D003580:Cytochromes / Q000378:metabolism; D004734:Energy Metabolism; D007103:Immobilization / Q000502:physiology; D008928:Mitochondria / Q000378:metabolism*; D010101:Oxygen Consumption; D051381:Rats; D017208:Rats, Wistar / Q000254:growth & development*; D012741:Sexual Maturation
D003580:Cytochromes
Izmenenie struktury dykhatel'noĭ tsepi mitokhondriĭ iz razlichnykh tkaneĭ v zavisimosti ot usloviĭ razvitiia mlekopitaiushchikh.