Radioimmunoimaging of human breast carcinoma xenografts in nude mouse model with 111In-labeled new monoclonal antibody EBA-1 and F(ab')2 fragments.
Radioimmunoimaging characteristics of a new monoclonal antibody EBA-1 and its F(ab')2 fragments utilizing nu/nu mice bearing human breast carcinoma xenografts are described. 111In-DTPA conjugates of EBA-1 localized with tumor/blood ratios of 0.99 +/- 0.10 (P < 0.3) and 4.65 +/- 1.60 (P < 0.05), and localization indices of 1.25 and 2.61 at 24 and 72 h, respectively. A tumor/blood ratio as high as 28.86 +/- 6.90 (P < 0.025) was obtained with EBA-1 F(ab')2 radioconjugates at 48 h. These results suggest that EBA-1 and its F(ab')2 might be useful reagents in radioimmunoimaging and radioimmunotherapy.
Yemul
S
S
Department of Surgery, Columbia University, New York, N.Y. 10032.
Leon
J A
JA
Pozniakoff
T
T
Esser
P D
PD
Estabrook
A
A
D000818:Animals; D000911:Antibodies, Monoclonal; D000915:Antibody Affinity; D015153:Blotting, Western; D001943:Breast Neoplasms / Q000000981:diagnostic imaging*; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D005260:Female; D006801:Humans; D007140:Immunoglobulin Fab Fragments; D007205:Indium Radioisotopes; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D004369:Pentetic Acid; D016719:Radioimmunodetection / Q000379:methods*; D014018:Tissue Distribution; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D007140:Immunoglobulin Fab Fragments; D007205:Indium Radioisotopes; D004369:Pentetic Acid