Ektachem slides overestimate serum albumin in patients with nephrotic syndrome.
In a non-selected patient population (n = 140), serum albumin concentrations obtained with Ektachem slides were very significantly correlated with results determined with serum protein electrophoresis on Olympus Hite 200. In 7 patients with nephrotic syndrome, however, no such correlation existed, concentrations on Ektachem being 20 to 200% higher (up to 16 g/l difference). In other samples, albumin concentrations on Ektachem were 10 g/l lower. Between-method differences appeared most closely correlated with the sum of alpha 1- and alpha 2-globulins as determined by serum protein electrophoresis. Immunonephelometric determination of various serum proteins demonstrated that haptoglobin concentrations most closely paralleled the observed between-method differences.
Gorus
F K
FK
Laboratorium Klinische Chemie, Vrije Universiteit Brussel, Belgium.
Van Blerk
M
M
D000704:Analysis of Variance; D001774:Blood Chemical Analysis / Q000295:instrumentation / Q000379:methods*; D001797:Blood Protein Electrophoresis; D001798:Blood Proteins / Q000032:analysis; D003124:Colorimetry; D006801:Humans; D009404:Nephrotic Syndrome / Q000097:blood*; D011897:Random Allocation; D012044:Regression Analysis; D012709:Serum Albumin / Q000032:analysis*
D001798:Blood Proteins; D012709:Serum Albumin