Physical mapping of 2000 kb of the mouse X chromosome in the vicinity of the Xist locus.
A physical map encompassing approximately 2.0 megabases (Mb) in the region of the mouse X-inactivation center has been constructed. The map extends from the Gjb-1 locus to the Xist locus and demonstrates the order of probes inseparable by genetic analysis. The deduced locus order is as follows: Gjb-1, Ccg-1, DXCrc171, Rps4, Phka, DXCrc177, DXCrc318, Xist. Detailed physical mapping in the region between the Phka and Xist loci indicates the position of CpG-rich islands associated with the 5' end of genes. The DXCrc177 and DXCrc318 loci, both defined by probes derived from linking clones, are associated with CpG-rich islands. The map provides a framework for the isolation of underlying sequences in the mouse X-inactivation center region.
Cooper
P
P
Section of Comparative Biology, MRC Clinical Research Centre, Harrow, United Kingdom.
Keer
J T
JT
McCabe
V M
VM
Hamvas
R M
RM
Brown
S D
SD
Rastan
S
S
Brockdorff
N
N
D000818:Animals; D001483:Base Sequence; D004277:DNA, Single-Stranded; D004303:Dosage Compensation, Genetic; D008040:Genetic Linkage; D051379:Mice; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D014960:X Chromosome
D004277:DNA, Single-Stranded