[Simultaneous measurement of kinetochore protein and DNA by flow cytometry].
We were successful in obtaining simultaneous measurements of kinetochore protein and DNA with flow cytometry using HeLa cells and KM cells; the latter originated with a squamous cell carcinoma of the oral cavity. Kinetochore protein was stained with the serum obtained from a patient with CREST syndrome and FITC-conjugated goat antihuman IgG antibody; the DNA was stained with propidium iodide. The FITC fluorescence of cells with a large DNA index was stronger than in cells with a small DNA index. The FITC intensity increased from G1 phase to G2/M phase. The rate of FITC intensity increase representing the kinetochore protein amount was greater in the early and late S phases than in the other phases. The results suggest that kinetochore protein increases in the interphase as the cell cycle unfolds.
Oga
A
A
Dept. of Pathology, Yamaguchi University School of Medicine.
Murakami
T
T
Kimura
Y
Y
Ida
M
M
Takahashi
M
M
D002294:Carcinoma, Squamous Cell / Q000473:pathology; D002453:Cell Cycle; D002503:Centromere / Q000737:chemistry*; D002868:Chromosomal Proteins, Non-Histone / Q000032:analysis*; D004273:DNA, Neoplasm / Q000032:analysis*; D005434:Flow Cytometry; D006367:HeLa Cells; D006801:Humans; D009062:Mouth Neoplasms / Q000473:pathology
D002868:Chromosomal Proteins, Non-Histone; D004273:DNA, Neoplasm