[Synthesis of oligodeoxyribothymidylate derivatives containing alkylating groups and residues of biotin, for directed modification of chromatin].
An oligodeoxythymidylate derivative bearing an alkylating group at the 5'-end and biotin at the 3'-end (RCl-(pdT)16-bio) was synthesized. This reagent alkylates polyA-tracts of DNA in HeLa nuclei specifically via complementary complexes with single-stranded segments of DNA and by the preliminary treatment of chromatin with S1-nuclease, since the reaction is inhibited by an excess of the corresponding free oligothymidylate. The reagent will be used to study the distribution of local unwinded parts of polyA-repeats of DNA in human chromatin by the electron microscopy.
Gorozhankin
A V
AV
Ivanova
E M
EM
Kobets
N D
ND
D000477:Alkylating Agents / Q000032:analysis*; D001710:Biotin; D002843:Chromatin / Q000737:chemistry*; D004247:DNA / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D009838:Oligodeoxyribonucleotides / Q000032:analysis / Q000138:chemical synthesis*
D000477:Alkylating Agents; D002843:Chromatin; D009838:Oligodeoxyribonucleotides; C027903:oligo (dT); D001710:Biotin; D004247:DNA
Sintez proizvodnogo oligodezoksiribotimidilata, soderzhashchego alkiliruiushchuiu gruppu i ostatok biotina, dlia napravlennoĭ modifikatsii khromatina.