Controlled release of proteins from 2-hydroxyethyl methacrylate copolymer gels.
A series of hydrogels with large pores was synthesized by the precipitation polymerization of 2-hydroxyethyl methacrylate (HEMA) with crosslinking agent in aqueous solution. Such gels are potentially useful for the controlled release of large-molecular-weight species such as proteins. In this study, the release behavior of lysozyme and alpha-amylase from hydrogels formed from HEMA or HEMA with a comonomer was studied. It was found that the polymer composition affected the total amount of lysozyme released and its activity. Effects were smaller with alpha-amylase. Charged gels, containing a phosphate moiety, released larger amounts of lysozyme at a reduced rate as a result of charge-charge interactions.
Antonsen
K P
KP
Center for Bioengineering, University of Washington, Seattle 98195.
Bohnert
J L
JL
Nabeshima
Y
Y
Sheu
M S
MS
Wu
X S
XS
Hoffman
A S
AS
D001672:Biocompatible Materials; D003692:Delayed-Action Preparations; D016503:Drug Delivery Systems; D005782:Gels; D008689:Methacrylates; D009113:Muramidase / Q000008:administration & dosage*; D000516:alpha-Amylases / Q000008:administration & dosage
D001672:Biocompatible Materials; D003692:Delayed-Action Preparations; D005782:Gels; D008689:Methacrylates; C005044:hydroxyethyl methacrylate; D000516:alpha-Amylases; D009113:Muramidase