Immunization of rabbits with proteins reacted with malonic dialdehyde (MDA): kinetics and specificity of the immune response.
MDA-modified casein, lysozyme or polylysine (MC, ML and MP respectively), was intradermically injected to rabbits in the presence of complete Freund's adjuvant (cFA). Two other animal sets received either cFA alone, or MDA alone. MDA, cFA and MP did not induce any antibody response. Both ML and MC produced an increase of antibody reactivity towards ML, but reactivity towards native lysozyme (L) was increased only by ML and not by MC. According to these results, it was concluded that the epitopes recognized by antibodies reacting with ML and not with L are AIP bridges and possibly the two surrounding aminoacyl (especially lysyl) residues.
Chancerelle
Y
Y
Centre de Recherches du Service de Santé des Armées, La Tronche, France.
Mathieu
J
J
Viret-Soropogui
R
R
Tosetti
F
F
Alban
C
C
Kergonou
J F
JF
D000818:Animals; D000917:Antibody Formation; D000918:Antibody Specificity; D002364:Caseins / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005620:Freund's Adjuvant / Q000276:immunology; D007700:Kinetics; D008297:Male; D008315:Malondialdehyde / Q000276:immunology*; D009113:Muramidase / Q000276:immunology; D011107:Polylysine / Q000276:immunology; D011506:Proteins / Q000276:immunology*; D011817:Rabbits; D014611:Vaccination
D002364:Caseins; D011506:Proteins; D011107:Polylysine; D008315:Malondialdehyde; D005620:Freund's Adjuvant; D009113:Muramidase