An interspecies idiotope associated with the anti-cholera toxin response detected by a monoclonal auto-anti-idiotypic antibody.
We have produced an auto-anti-idiotypic (auto-anti-id) monoclonal antibody (mAb) which reacted with syngenic mouse polyclonal anti-cholera toxin (anti-CT) IgG antibody (Ab) and six/eight different anti-CT IgG mAb, but not with normal mouse BALB/c IgG. The binding of this auto-anti-id mAb on one anti-CT mAb was significantly inhibited by polyclonal anti-CT sera of rats, rabbits and mice. Thus the idiotope on anti-CT Ab recognized by this auto-anti-id mAb was public and expressed in different species. Because of the absence of competition between CT and this auto-anti-id mAb for the binding to the six anti-CT mAb, the anti-id mAb was classified as an Ab2 alpha. The efficiency of this auto-anti-id mAb to induce anti-CT Ab3 was tested with success in rabbits and rats. Auto-anti-id mAb-immunized rats were significantly protected against an intraintestinal CT challenge.
Lucas
G P
GP
International Institute of Cellular and Molecular Pathology, Brussels, Belgium.
Cambiaso
C L
CL
Vaerman
J P
JP
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology; D000907:Antibodies, Bacterial / Q000096:biosynthesis / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology; D002771:Cholera / Q000517:prevention & control; D002772:Cholera Toxin / Q000276:immunology*; D003429:Cross Reactions; D007114:Immunization; D007130:Immunoglobulin Idiotypes / Q000032:analysis*; D007422:Intestines / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D011817:Rabbits; D051381:Rats; D013045:Species Specificity
D000888:Antibodies, Anti-Idiotypic; D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D007130:Immunoglobulin Idiotypes; D002772:Cholera Toxin