title: Isolation of two members of the rat MAP kinase kinase gene family.
abstract: Mitogen-activated protein (MAP) kinase kinase (MAPKK) is a recently characterized activator of MAP kinase (MAPK), and is considered to be regulated by a protooncogene product c-Raf-1. It is, however, unclear whether the signals originating from c-Raf-1 utilize this phosphorylation cascade to lead to oncogenesis. To clarify this point, we isolated rat MAPKK cDNAs, and identified two distinct cDNAs encoding MAPKK and a highly related kinase, both with molecular weights of approximately 45 kDa (MEK1 and MEK2). Genomic Southern blot analyses suggested that MAPKK may form a large gene family.
authors: Okayama Cell Switching Project, Research and Development Corporation of Japan (JRDC), Kyoto.
authors: M
authors: M
authors: Otsu
authors: Y
authors: Y
authors: Terada
authors: H
authors: H
authors: Okayama
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D015870:Gene Expression; D005796:Genes; D020929:Mitogen-Activated Protein Kinase Kinases; D008969:Molecular Sequence Data; D005810:Multigene Family; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D016133:Polymerase Chain Reaction; D011494:Protein Kinases / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D015183:Restriction Mapping; D016415:Sequence Alignment
chemical_list: D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger; D004247:DNA; D011494:Protein Kinases; D020929:Mitogen-Activated Protein Kinase Kinases