Isolation of a myotoxin from the venom of Agkistrodon contortrix laticinctus (broad-banded copperhead) and pathogenesis of myonecrosis induced by it in mice.
A myotoxin was isolated from the venom of the broad-banded copperhead (Agkistrodon contortrix laticinctus) by HPLC using anion and cation exchange chromatography. The toxin has a mol. wt of approximately 14,000 and has a pI greater than 9. It does not have phospholipase A activity, but does induce myonecrosis of skeletal muscle cells characterized by a hypercontraction of myofilaments. Electron microscopic analysis showed that the myotoxin appears to disrupt the sarcolemma of skeletal muscle cells. ACL myotoxin is very similar in mol. wt, amino acid composition, and myotoxic activity to myotoxins isolated from the venoms of Bothrops asper and Bothrops nummifer from Central America, suggesting that homologs of this toxin may be found in other crotaline snake venoms.
Johnson
E K
EK
Department of Physiological Sciences, Oklahoma State University, Stillwater 74078.
Ownby
C L
CL
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002848:Chromatography, DEAE-Cellulose; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D003435:Crotalid Venoms / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D007525:Isoelectric Focusing; D051379:Mice; D008854:Microscopy, Electron; D008970:Molecular Weight; D009132:Muscles / Q000473:pathology; D009135:Muscular Diseases / Q000139:chemically induced* / Q000473:pathology; D010740:Phospholipases / Q000378:metabolism; D014118:Toxins, Biological / Q000302:isolation & purification* / Q000378:metabolism / Q000633:toxicity
C031442:Agkistrodon venoms; D000596:Amino Acids; D003435:Crotalid Venoms; D014118:Toxins, Biological; D010740:Phospholipases