Early-onset autoimmune hepatitis is associated with a C4A gene deletion.
BACKGROUND
Autoimmune hepatitis is an immunologically mediated disorder with some similarities to systemic lupus erythematosus, including an association with HLA-A1, B8, DR3. This haplotype includes a C4A, 21-OHA gene deletion. Low serum levels of complement and C4 null alleles have been reported in autoimmune hepatitis, but studies have been at the protein level only.


METHODS
Twenty-four white patients with autoimmune hepatitis were studied by Southern blots using a C4A gene complementary DNA probe. HLA A, B, and C typing was determined using standard microcytotoxicity assays, and DR and DQ specificities were determined by restriction fragment length polymorphism analysis.


RESULTS
Thirteen of 24 patients had the C4A gene deletion compared with 12 of 90 controls. HLA-A1 and B8 were increased in patients with autoimmune hepatitis, as were HLA-DR3 (DR17), Dw24, DQ2. Patients with a C4A gene deletion presented at a younger age than those without the deletion and had significantly lower serum C3 and C4 levels. The C4A gene deletion was associated with HLA-A1, B8, DR3 in all but 1 patient who was HLA-DR3 negative.


CONCLUSIONS
A C4A gene deletion is found in patients with autoimmune hepatitis, especially those presenting at a young age. This complement gene deletion may be an important factor in the development of this disease.
Scully
L J
LJ
Department of Medicine, Ottawa Civic Hospital, Ontario, Canada.
Toze
C
C
Sengar
D P
DP
Goldstein
R
R
D000293:Adolescent; D000328:Adult; D001327:Autoimmune Diseases / Q000235:genetics* / Q000503:physiopathology; D015139:Blotting, Southern; D015934:Complement C4a / Q000235:genetics*; D017353:Gene Deletion; D006680:HLA Antigens / Q000145:classification; D006505:Hepatitis / Q000235:genetics* / Q000503:physiopathology; D006801:Humans; D008875:Middle Aged; D044465:White People
D006680:HLA Antigens; D015934:Complement C4a