Comparative study of the isolation of human immunodeficiency virus from the lung and peripheral blood of AIDS patients.
HIV-1 proviral DNA was detected by the polymerase chain reaction (PCR) in leucocytes from the peripheral blood (PBL) and bronchoalveolar lavage (BAL) of 100% and 72%, respectively, of HIV-1 seropositive patients. Infective virus isolated by cocultivation was recovered from the PBL and BAL of 50% and 59% of individuals, respectively. Isolation of HIV-1 was more readily made from the lung of individuals undergoing Pneumocystis carinii pneumonia (PCP) than from patients with non-PCP lung infections. The concomitant infection of lung cells with cytomegalovirus did not affect the isolation rate of HIV-1 from the lung. HIV-1 was isolated from BAL of 23 out of 36 (64%) individuals receiving no antiviral chemotherapy and from 13 out of 24 (54%) patients who were receiving AZT.
Clarke
J R
JR
Department of Medical Microbiology, St Mary's Hospital Medical School, London, England.
Williamson
J D
JD
Mitchell
D M
DM
D017088:AIDS-Related Opportunistic Infections / Q000382:microbiology; D000163:Acquired Immunodeficiency Syndrome / Q000188:drug therapy / Q000382:microbiology*; D001992:Bronchoalveolar Lavage Fluid / Q000382:microbiology; D004279:DNA, Viral / Q000097:blood / Q000302:isolation & purification; D006679:HIV Seropositivity / Q000188:drug therapy / Q000382:microbiology; D015497:HIV-1 / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008168:Lung / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D014766:Viremia / Q000382:microbiology; D015215:Zidovudine / Q000627:therapeutic use
D004279:DNA, Viral; D015215:Zidovudine