Flow injection fluorescence microscopy: a novel tool for the study of cells through controlled perfusion.
Current methods of microscope stage perfusion do not take full advantage of existing technology for precise fluid control. The concept of flow injection, used extensively by analytical chemists, is described and its application to the fluorescence microscopic study of cultured cells is proposed. Using this technique, cells may be exposed to single or multiple reagent zones of almost any profile, sequence, and duration, with computer-controlled precision. A flow injection system is employed in conjunction with a novel perfusion chamber--the fountain cell. The ability of the flow injection system to perfuse cells with a reagent with a reproducibility of 1% RSD is demonstrated. The system was used to monitor changes in calcium levels in baby hamster kidney cells loaded with FURA-2 as a result of stimulation with a precisely timed concentration of ionomycin. The unique feature of the technique is that it allows a series of responses of a given cell to be directly compared to each other.
Scudder
K M
KM
Department of Chemistry, University of Washington, Seattle 98195.
Christian
G D
GD
Ruzicka
J
J
D000818:Animals; D002118:Calcium / Q000378:metabolism; D002460:Cell Line; D006224:Cricetinae; D005434:Flow Cytometry / Q000295:instrumentation*; D016257:Fura-2; D066298:In Vitro Techniques; D015759:Ionomycin / Q000494:pharmacology; D007668:Kidney / Q000166:cytology; D008856:Microscopy, Fluorescence / Q000295:instrumentation*; D010477:Perfusion; D013997:Time Factors
D015759:Ionomycin; D002118:Calcium; D016257:Fura-2