title: Detailed characterization of a human 8q24.1 microdissection library and generation of "sequence-tagged sites".
abstract: A total of 533 clones from a human 8q24.1 microdissection library was analyzed by automatic DNA sequencing. Three hundred and thirty-seven different insert sequences were found. The insert size ranged from 45 to 376 bp (mean, 170 bp). Eighty-six percent (291/337) of these sequences were free of repetitive DNA. Each of 19 clones tested was successfully translated into a sequence-tagged site. We conclude that the microdissection library is a rich source of DNA markers for 8q24.1.
authors: Institut für Humangenetik, Universitätsklinikum Essen, FRG.
authors: B
authors: B
authors: La Pillo
authors: S
authors: S
authors: Karpinski
authors: H J
authors: HJ
authors: Lüdecke
authors: B
authors: B
authors: Horsthemke
mesh_terms: D001483:Base Sequence; D002874:Chromosome Mapping; D002898:Chromosomes, Human, Pair 8 / Q000648:ultrastructure*; D005819:Genetic Markers; D015698:Genomic Library; D006801:Humans; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D016324:Sequence Tagged Sites
chemical_list: D005819:Genetic Markers; D009838:Oligodeoxyribonucleotides