title: Relationship of secretory granule content and proliferative intensity in the secretory compartment of the hamster bronchial epithelium.
abstract: We have previously shown that normal hamster airway epithelial secretory cells have a lower proliferative intensity than basal cells, but because of their high frequency are a major contributor to cell renewal (Am. J. Respir. Cell Mol. Biol. 1990; 2:51-58). In the present experiment, the relation between proliferative intensity and secretory granule content in bronchial epithelial cells is studied. [3H]thymidine (2 microCi/g wt) was given intraperitoneally, 1 h before killing, to 5 hamsters treated 21 days earlier with intratracheal saline and to six hamsters in which secretory cell metaplasia had been induced by intratracheal treatment with 300 micrograms of human neutrophil elastase given 21 days earlier. Light microscopic autoradiograms were prepared from 2-microns-thick Epon sections of left intrapulmonary hilar bronchi. Cells were categorized as basal, ciliated, secretory, or indeterminate. Secretory cells were classified as either: S1, with 0 to 4 granules; S2, with > or = 5 granules with intervening cytoplasm; or S3, with abundant granules and no apparent supranuclear cytoplasm. Proliferative intensity was defined by the categorical labeling index (LIc) at 1 h after [3H]thymidine injection. LIc was determined by the number of labeled cells in a category as the percent of labeled and unlabeled cells of that category. LIc of each of the cell categories were similar in the elastase and saline groups. LIc was highest for basal cells, reflecting their proliferative intensity, and lowest for ciliated cells. In the saline group, LIc of S1 (0.25%) was significantly higher compared with S2 (0.13%); S3 cells were rare (0.2%) and none were labeled.(ABSTRACT TRUNCATED AT 250 WORDS)
authors: Pulmonary Institute, Hebrew University-Hadassah Medical School, Jerusalem, Israel.
authors: R
authors: R
authors: Breuer
authors: T G
authors: TG
authors: Christensen
authors: Y
authors: Y
authors: Wax
authors: G
authors: G
authors: Bolbochan
authors: E C
authors: EC
authors: Lucey
authors: P J
authors: PJ
authors: Stone
authors: G L
authors: GL
authors: Snider
mesh_terms: D000818:Animals; D001980:Bronchi / Q000166:cytology* / Q000648:ultrastructure; D002455:Cell Division; D006224:Cricetinae; D003594:Cytoplasmic Granules; D004847:Epithelial Cells; D004848:Epithelium / Q000648:ultrastructure; D006801:Humans; D008297:Male; D008647:Mesocricetus; D008854:Microscopy, Electron; D010196:Pancreatic Elastase
chemical_list: D010196:Pancreatic Elastase