Cloning and sequence determination of the valS gene, encoding valyl-tRNA synthetase in Lactobacillus casei.
The DNA sequence of the valS gene from Lactobacillus casei and the predicted amino acid sequence of its valyl-tRNA synthetase product have been determined. An open reading frame coding for a protein of 901 amino acids was found. A clone containing the intact L. casei valS gene functionally complemented the temperature-sensitive growth of the valS mutant strain 236c of Escherichia coli. The valS gene and the downstream folylpolyglutamate synthetase gene are transcribed in the same direction but are separated by a putative transcription terminator.
Taylor
B V
BV
Department of Microbiology, University of Toronto, Ontario, Canada.
Toy
J
J
Sit
T L
TL
Bognar
A L
AL
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D007780:Lacticaseibacillus casei / Q000201:enzymology / Q000235:genetics*; D008969:Molecular Sequence Data; D010957:Plasmids; D014637:Valine-tRNA Ligase / Q000737:chemistry / Q000235:genetics*
D014637:Valine-tRNA Ligase