Enhancement of intracellular uncoating of adenovirus in HeLa cells in the presence of benzyl alcohol as a membrane fluidizer.
The early extra- and intra-cellular interaction between adenovirus type 2 and HeLa cells was studied in the presence of benzyl alcohol as a fluidizing agent. The process of virus attachment and internalization were not affected at 5-15 mM of benzyl alcohol at 25 degrees C. Under the same conditions an enhancement by 45% at the most was demonstrated for the cell-mediated virion uncoating. By completely blocking virion internalization with 50 mM azide the uncoating was reduced to 20% of the normal level. The remaining surface-located uncoating was not affected by benzyl alcohol. It was demonstrated that an enhancement of the intracellular virion uncoating was followed by a raised production of the hexon antigen, which was interpreted as an increase in the specific infectivity of the virus.
Blixt
Y
Y
Department of Microbiology, University of Lund, Sweden.
Varga
M J
MJ
Everitt
E
E
D000256:Adenoviridae / Q000502:physiology*; D000956:Antigens, Viral / Q000096:biosynthesis; D019905:Benzyl Alcohol; D001592:Benzyl Alcohols / Q000494:pharmacology*; D002448:Cell Adhesion; D002462:Cell Membrane / Q000187:drug effects / Q000382:microbiology*; D004705:Endocytosis; D006367:HeLa Cells; D006801:Humans; D008560:Membrane Fluidity / Q000187:drug effects; D014168:Transferrin / Q000493:pharmacokinetics
D000956:Antigens, Viral; D001592:Benzyl Alcohols; D014168:Transferrin; D019905:Benzyl Alcohol