Altered growth and spontaneous transformation of cells cultured from v-jun transgenic mice recapitulate wound-induced multistage tumorigenesis.
H-2K/v-jun transgenic mice develop sarcomas at sites of wounding via a multistep process characterized by discrete pathological stages. To study this progression in vitro, cells from different stages of tumorigenesis were cultured and examined for their growth properties. The results show that whereas transgenic fibroblasts do not manifest enhanced proliferative potential in vivo in the absence of wounding, they do show obvious proliferative advantage relative to nontransgenic fibroblasts in vitro, including the capacity for indefinite growth. In addition, relative to nontransgenic fibroblasts, transgenic cells show altered sensitivity to platelet-derived growth factor and tumor necrosis factor alpha, both of which are known to be mobilized during wounding. No obvious differences in growth potential are observed between transgenic fibroblasts and cells cultured from wound-induced premalignant lesions, and confluent cultures of both cell populations give rise to spontaneous foci of transformed myogenic and nonmyogenic cells that resemble those of late-stage malignant wound sarcomas. Relative to transgenic fibroblast cultures, however, premalignant lesion cultures segregate transformed cells at a greater frequency and after shorter intervals of in vitro growth. The results suggest that wound-induced multistage tumorigenesis can be recapitulated in vitro and that cells cultured from different stages of tumorigenesis retain biological properties that reflect the pathological stage from which they are derived.
Schuh
A C
AC
Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada.
Theodorescu
D
D
Shalaby
F
F
Breitman
M L
ML
D000818:Animals; D002455:Cell Division / Q000502:physiology; D002461:Cell Line, Transformed / Q000502:physiology; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology*; D016758:Genes, jun; D006133:Growth Substances / Q000502:physiology; D051379:Mice; D008822:Mice, Transgenic; D024510:Muscle Development; D009367:Neoplasm Staging; D009374:Neoplasms, Experimental / Q000209:etiology* / Q000473:pathology / Q000503:physiopathology; D014947:Wounds and Injuries / Q000150:complications* / Q000503:physiopathology
D006133:Growth Substances