title: Role of granulocyte-macrophage colony-stimulating factor, interleukin-3 and interleukin-5 in the eosinophilia associated with T cell lymphoma.
abstract: We studied two patients with a leukaemic T cell lymphoma who presented with a marked increase in blood eosinophilia. To investigate the mechanism of the eosinophilia, supernatants of peripheral blood cells containing more than 80% lymphoma cells were tested by biological assays for the presence of colony stimulating factors (CSF). In one case supernatants stimulated the growth of granulocyte-macrophage (GM), erythroid and eosinophil colonies. These effects were neutralized by anti-GM-CSF antibodies; anti-IL5 antibodies slightly decreased eosinophil colony formation. Supernatants derived from the second patient cells stimulated the same lineages. Neutralizing experiments demonstrated that in addition to GM-CSF it contained interleukin 3 (IL-3) and interleukin 5 (IL-5). In agreement with the biological data, RNA studies using the polymerase chain reaction showed that cells from the first patient expressed GM-CSF transcripts; IL-5 transcripts were also detected in very low amounts. GM-CSF, IL-3 and IL-5 transcripts were detected in cells from the second patient. Thus eosinophilia associated with some T cell lymphoma is likely due to secretion of different combinations of cytokines by malignant cells.
authors: Service d'Immuno-Hématologie, Hôpital Saint Louis, Paris, France.
authors: J P
authors: JP
authors: Fermand
authors: M T
authors: MT
authors: Mitjavila
authors: J P
authors: JP
authors: Le Couedic
authors: A
authors: A
authors: Tsapis
authors: R
authors: R
authors: Berger
authors: R
authors: R
authors: Modigliani
authors: M
authors: M
authors: Seligmann
authors: J C
authors: JC
authors: Brouet
authors: W
authors: W
authors: Vainchenker
mesh_terms: D000328:Adult; D001483:Base Sequence; D004273:DNA, Neoplasm / Q000032:analysis; D004802:Eosinophilia / Q000235:genetics / Q000276:immunology*; D005260:Female; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000097:blood*; D006801:Humans; D007377:Interleukin-3 / Q000097:blood*; D015848:Interleukin-5 / Q000097:blood*; D007414:Intestinal Neoplasms / Q000235:genetics / Q000276:immunology; D016399:Lymphoma, T-Cell / Q000235:genetics / Q000276:immunology*; D016410:Lymphoma, T-Cell, Cutaneous / Q000276:immunology; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012334:RNA, Neoplasm / Q000032:analysis; D012878:Skin Neoplasms / Q000235:genetics / Q000276:immunology
chemical_list: D004273:DNA, Neoplasm; D007377:Interleukin-3; D015848:Interleukin-5; D012334:RNA, Neoplasm; D016178:Granulocyte-Macrophage Colony-Stimulating Factor