title: Inhibitory effect of oolong tea polyphenols on glycosyltransferases of mutans Streptococci.
abstract: Oolong tea extract (OTE) was found to inhibit the water-insoluble glucan-synthesizing enzyme, glucosyltransferase I (GTase-I), of Streptococcus sobrinus 6715. The GTase-inhibitory substance in the OTE was purified successive adsorption chromatography on Diaion HP-21 and HP-20 columns; this was followed by further purification by Sephadex LH-20 column chromatography. A major fraction that inhibited GTase activity (fraction OTF10) was obtained, and the chemical analysis of OTF10 indicated that it was a novel polymeric polyphenol compound that had a molecular weight of approximately 2,000 and differed from other tea polyphenols. Catechins and all other low-molecular-weight polyphenols except theaflavin derived from balck tea did not show significant GTase-inhibitory activities. It was found that OTE amd PTF10 markedly inhibit GTase-I and yeast alpha-glucosidase, but not salivary alpha-amylase. Various GTases purified from S. sobrinus and Streptococcus mutans were examined for inhibition by OTE and OTF10. It was determined that S. sobrinus GTase-I and S. mutans cell-free GTase synthesizing water-soluble glucan were most susceptible to the inhibitory action of OTF10, while S. sobrinus GTase-Sa and S. mutans cell-associated GTase were moderately inhibited; no inhibition of S. sobrinus GTase-Sb was observed. Inhibition of a specific GTase or specific GTases of mutants streptococci resulted in decreased adherence of the growing cells of these organisms. The inhibitory effect of OTF10 on cellular adherence was significantly stronger than that of OTE.
authors: Institute for Biomedical, Suntory Ltd., Shimamotocho, Osaka 618, Japan.
authors: K
authors: K
authors: Nakahara
authors: S
authors: S
authors: Kawabata
authors: H
authors: H
authors: Ono
authors: K
authors: K
authors: Ogura
authors: T
authors: T
authors: Tanaka
authors: T
authors: T
authors: Ooshima
authors: S
authors: S
authors: Hamada
mesh_terms: D001422:Bacterial Adhesion / Q000187:drug effects; D002392:Catechin / Q000494:pharmacology; D005419:Flavonoids; D005936:Glucans / Q000096:biosynthesis; D005964:Glucosyltransferases; D010636:Phenols / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D011108:Polymers / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D013295:Streptococcus mutans / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism; D013662:Tea / Q000737:chemistry*
chemical_list: D005419:Flavonoids; D005936:Glucans; D010636:Phenols; D011108:Polymers; D013662:Tea; D002392:Catechin; D005964:Glucosyltransferases