[Heat inactivation kinetics of solubilized and immobilized immunoglobulins G].
The kinetics of thermoinactivation of rabbit antibodies against cortisol (Anti-COR, 60-82 degrees), sheep antibodies against rabbit immunoglobulins (AB, 65-77 degrees) in water solution and of AB covalently linked to activated polystyrene beads (PSB-AB, 60-77 degrees) was studied by indirect and direct ELISA methods using peroxidase-cortisol or peroxidase-rabbit immunoglobulin conjugates to detect immunocomplexes for PSB-AB. High stability of ABs and their stabilization after immobilization on PSB are shown. The Eact values and thermodynamic activation parameters of immunoglobulins thermolysis delta H*, delta S* and delta G* were calculated from Arrhenius plots of the effective inactivation rate constants versus temperature and compared with the corresponding characteristics for other proteins.
Markina
V L
VL
Eremin
A N
AN
Metelitsa
D I
DI
D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay; D006358:Hot Temperature; D006854:Hydrocortisone / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology*; D007700:Kinetics; D011817:Rabbits; D013816:Thermodynamics
D007074:Immunoglobulin G; D006854:Hydrocortisone
Kinetika termoinaktivatsii rastvorennykh i immobilizovannykh immunoglobulinov G.