title: [Biosynthesis of bovine growth hormone in Escherichia coli DH1 cells and its purification on a pilot scale].
abstract: The biosynthesis of the bovine growth hormone (BGH) in E. coli DH1 cells containing the plasmid pbGH(1-119)ptrp was studied. It was found that BGH accumulated in the cytoplasm of bacterial cells as optically dense granules that can be isolated by low-speed centrifugation. A fraction of purified protein granules was used for further purification of BGH. A purified BGH preparation contained two polypeptides with molecular weights of 18,000 and 22,000 D. The state of plasmid was investigated during cultivation and storage of the culture.
authors: V M
authors: VM
authors: Nikolaeva
authors: S V
authors: SV
authors: Bykovskaia
authors: V A
authors: VA
authors: Kozhin
authors: S M
authors: SM
authors: Maĭorov
authors: T G
authors: TG
authors: Usankina
authors: L G
authors: LG
authors: Rukhlova
authors: I E
authors: IE
authors: Luzina
authors: V A
authors: VA
authors: Ezhov
mesh_terms: D000818:Animals; D002417:Cattle; D004926:Escherichia coli / Q000648:ultrastructure; D013006:Growth Hormone / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification; D008854:Microscopy, Electron; D010865:Pilot Projects; D010957:Plasmids
chemical_list: D013006:Growth Hormone
vernacular_title: Biosintez bych'ego gormona rosta v kletkakh Escherichia coli DH1 i ego vydelenie na pilotnom urovne.