title: [Diagnosis of anaerobic infection of the pleural fluid using gas-liquid chromatography].
abstract: BACKGROUND
  An assessment of the efficacy of the detection of volatile and non-volatile fatty acids that are produced by microorganisms aimed at the possible diagnosis of pleural empyema.
  
  
  METHODS
  106 pleural fluids were examined by gas-liquid chromatography in contrast to conventional aerobic and anaerobic cultures. The sample previously prepared (1 microliter) was injected in an Hewlett-Packard 5890 chromatograph, that was equipped with a fused silica capillary column and a flame ionization detector.
  
  
  RESULTS
  Growth of aerobic or anaerobic microorganisms was obtained in 13 (12.3%) of the samples, and volatile or non-volatile fatty acids were detected in all of them (sensitivity = 1). No growth was obtained in the 93 samples, but volatile or non-volatile fatty acids were detected in 64 (68.8%) of them (specificity = 0.31). A positive predictive value of 0.17 and a negative predictive value of 1 were obtained. Growth of anaerobic microorganisms was obtained in 9 samples (8.5%) and volatile fatty acids were detected in all of them (sensitivity = 1). Anaerobic microorganisms did not grow in 97 samples, however volatile fatty acids were detected in 2 (2.1%) of them. A positive predictive value of 0.82 and a negative of 1 were obtained.
  
  
  CONCLUSIONS
  Referring to pleural fluid samples, the detection of volatile and non-volatile fatty acids by gas-liquid chromatography is a highly sensitive method, that may be used to quickly reject negative samples in about 30 mins. Furthermore, when anaerobic microorganisms exist, the high specificity of gas-liquid chromatography will make it possible to confirm their presence in the sample, mainly if there is a clinical suspect of anaerobic infection. Thus, the utilization of this sensitive method may also change the therapeutic behaviour.
authors: Servicio de Microbiología, Hospital Civil de Bilbao.
authors: M
authors: M
authors: Alvarez
authors: P
authors: P
authors: Rojo
authors: M
authors: M
authors: Latorre
authors: O
authors: O
authors: Herrero
authors: I
authors: I
authors: Arriaga
authors: J M
authors: JM
authors: Merino
authors: R
authors: R
authors: Cisterna
mesh_terms: D001420:Bacteria, Aerobic / Q000302:isolation & purification; D001421:Bacteria, Anaerobic / Q000737:chemistry / Q000302:isolation & purification*; D001424:Bacterial Infections / Q000175:diagnosis* / Q000382:microbiology; D002849:Chromatography, Gas; D016724:Empyema, Pleural / Q000175:diagnosis* / Q000382:microbiology; D005232:Fatty Acids, Volatile / Q000032:analysis*; D006801:Humans; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity
chemical_list: D005232:Fatty Acids, Volatile
vernacular_title: Diagnóstico de la infección anaerobia del líquido pleural mediante cromatografía de gas-líquido.