title: Decreased responsiveness to progesterone of spermatozoa in oligozoospermic patients.
abstract: Spermatozoa from oligozoospermic subjects are characterized by a reduced in vitro ability to penetrate hamster oocytes and by a decreased responsiveness to physiological stimuli that trigger the acrosome reaction. One of the first steps in the induction of the acrosome reaction is an increase of intracellular free calcium concentrations ([Ca2+]i). It has been recently shown that progesterone (P) is able to increase [Ca2+]i in capacitated human sperm at concentrations similar to those found in follicular fluid. We evaluated sperm [Ca2+]i increase in response to P (0.1 micrograms/ml) in 19 normo- and 17 oligozoospermic subjects. The average percentage of [Ca2+]i increase over the basal level was significantly lower in spermatozoa from oligozoospermic subjects when compared to normozoospermic subjects (138.7 +/- 8.22% increase in oligo- versus 263.3 +/- 39.7% increase in normozoospermic subjects; P < 0.001). Progesterone-stimulated [Ca2+]i increase was significantly correlated with sperm motility (r = 0.54), sperm concentration (r = 0.96), and sperm morphology (% of normal forms) (r = 0.49). In addition P induced a significant increase of acrosome-reacted spermatozoa in normospermic patients (n = 10), whereas no significant effect was observed in spermatozoa from oligozoospermic men (n = 7). Taken together, these results indicate that spermatozoa from oligozoospermic men have a reduced ability to initiate the cascade of events that lead to the acrosome reaction in response to a physiological stimulus, such as P, and might contribute to explaining the reduced fertilizing capacity of these patients.
authors: Department of Clinical Pathophysiology, University of Florence, Italy.
authors: C
authors: C
authors: Falsetti
authors: E
authors: E
authors: Baldi
authors: C
authors: C
authors: Krausz
authors: R
authors: R
authors: Casano
authors: P
authors: P
authors: Failli
authors: G
authors: G
authors: Forti
mesh_terms: D000177:Acrosome / Q000187:drug effects / Q000502:physiology; D002118:Calcium / Q000032:analysis / Q000378:metabolism; D006801:Humans; D008297:Male; D009845:Oligospermia / Q000503:physiopathology*; D011374:Progesterone / Q000494:pharmacology*; D013081:Sperm Motility / Q000187:drug effects / Q000502:physiology; D013094:Spermatozoa / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology
chemical_list: D011374:Progesterone; D002118:Calcium