Local and systemic immunity against Streptococcus pneumoniae: humoral responses against a non-capsulated temperature-sensitive mutant.
Temperature-sensitive mutants of Streptococcus pneumoniae were isolated after chemical mutagenesis. Intranasal immunization with temperature-sensitive mutant J/3 induced higher levels of circulating antibody than those obtained after immunization with the heat-killed parental wild type. Moreover, local immunization with mutant J/3 induced high levels of anti-S. pneumoniae IgG and IgA in the lower respiratory tract, whereas only moderate IgG (and no IgA) antibodies were detected in lung lavage fluids from mice immunized intranasally with the heat-killed strain.
García
V E
VE
Department of Microbiology, Parasitology and Immunology, School of Medicine, University of Buenos Aires, Argentina.
Gherardi
M M
MM
Iglesias
M F
MF
Cerquetti
M C
MC
Sordelli
D O
DO
D000281:Administration, Intranasal; D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis* / Q000097:blood; D000917:Antibody Formation; D016667:Bacterial Capsules / Q000276:immunology*; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology; D007110:Immunity, Active; D007070:Immunoglobulin A / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis; D008168:Lung / Q000276:immunology; D051379:Mice; D008815:Mice, Inbred Strains; D009092:Mucous Membrane / Q000276:immunology; D016296:Mutagenesis; D011008:Pneumococcal Infections / Q000235:genetics / Q000276:immunology*
D000907:Antibodies, Bacterial; D007070:Immunoglobulin A; D007074:Immunoglobulin G