Protective opsonic activity of antibodies against fibronectin-binding proteins (FnBPs) of Staphylococcus aureus.
In this report, opsonic activity of hyperimmune rabbit IgG against fibronectin-binding proteins (gal-FnBP A and ZZ-FnBP B) of Staphylococcus aureus is described. Moreover, the action of IgG purified from serum of rabbits immunized with 'combined vaccine' (fibronectin-binding protein A+collagen-binding protein+alpha-toxoid) is shown. The opsonic activity has been studied in an in vitro phagocytosis assay as well as in vivo. Mice which had been infected intraperitoneally with S. aureus strain Cowan 1 pretreated (opsonized in vitro) with specific anti-FnBPs IgG were able to eliminate the staphylococci from the peritoneal cavity and liver more rapidly than controls. Also, clearance from the bloodstream of intravenously injected S. aureus Cowan 1 as well as S. aureus U320, opsonized with IgG anti-FnBPs or anti-FnBP+CnBP+alpha-toxoid, was more effective than observed in control groups. In other in vivo experiments it was shown that mice passively immunized with hyperimmune IgG anti-FnBP (one or two doses, intravenously) before challenge with S. aureus Cowan I eliminated the bacteria better than controls injected only with preimmune IgG.
Rozalska
B
B
Department of Medical Microbiology, University of Lund, Sweden.
Wadström
T
T
D018829:Adhesins, Bacterial; D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology*; D000918:Antibody Specificity; D000942:Antigens, Bacterial / Q000276:immunology; D001425:Bacterial Outer Membrane Proteins / Q000096:biosynthesis / Q000276:immunology*; D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D002352:Carrier Proteins; D004195:Disease Models, Animal; D004306:Dose-Response Relationship, Immunologic; D007116:Immunization, Passive; D007140:Immunoglobulin Fab Fragments / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D008099:Liver / Q000382:microbiology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009895:Opsonin Proteins / Q000276:immunology*; D010537:Peritoneum / Q000382:microbiology; D010587:Phagocytosis; D013154:Spleen / Q000382:microbiology; D013203:Staphylococcal Infections / Q000097:blood / Q000276:immunology / Q000517:prevention & control; D013211:Staphylococcus aureus / Q000276:immunology* / Q000378:metabolism; D013997:Time Factors
D018829:Adhesins, Bacterial; D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D002352:Carrier Proteins; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D009895:Opsonin Proteins; C078635:fibronectin-binding proteins, bacterial