title: Identification of host-protective antigens of Taenia ovis oncospheres.
abstract: Sheep were fully protected against challenge infection following immunization with a homogenate of T. ovis oncospheres. Ultracentrifugation of sonicated oncospheres either alone or in the presence of a range of detergents did not reduce the immunogenicity of the extracts. Solubilization of oncosphere extracts in non-ionic detergents or sodium dodecyl sulphate (SDS) enabled analysis of host-protective antigens by isoelectric focusing (IEF) and electrophoresis in polyacrylamide gels (SDS-PAGE), respectively. Immunoblotting analysis of oncosphere antigens with immune sheep sera identified predominantly two groups of antigens with relative mobilities of 31-34 kDa and 47-52 kDa with a common isoelectric point of 5.8. The immunogenicity of these antigens was confirmed in vaccination trials using appropriate fractions cut from SDS-PAGE gels and agarose IEF gels. Affinity-purified antibodies prepared against the candidate antigens were used to select the corresponding recombinant DNA-derived polypeptides, one of which was subsequently found to be host-protective.
authors: Pitman-Moore New Zealand Limited, Research and Development Department, Upper Hutt, New Zealand.
authors: G B
authors: GB
authors: Harrison
authors: D D
authors: DD
authors: Heath
authors: R P
authors: RP
authors: Dempster
authors: S B
authors: SB
authors: Lawrence
authors: M W
authors: MW
authors: Lightowlers
authors: M D
authors: MD
authors: Rickard
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000276:immunology; D000917:Antibody Formation; D000947:Antigens, Helminth / Q000276:immunology* / Q000302:isolation & purification; D007116:Immunization, Passive; D012756:Sheep; D012757:Sheep Diseases / Q000276:immunology* / Q000517:prevention & control; D013621:Taenia / Q000276:immunology*; D013622:Taeniasis / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D014611:Vaccination
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth