[Characteristics of phospholipid hydrolysis kinetics by phospholipase C from Bacillus cereus. Hydrolysis of phosphatidylinositol in various aggregated states].
The process of phospholipase C hydrolysis of phosphatidylethanolamine (PE) in the form of mixed micelles phospholipid-detergent or in the form of vesicules in the mixture with phosphatidylcholine (PC) was studied. The size of the micelles was measured by dynamic light-scattering and their structure was determined by 31P-NMR spectroscopy. It was found that the kinetics of PE hydrolysis in the micelles by phospholipase C from Bacillus cereus do not follow Michaelis-Menten equation, but at all concentrations studied PE was hydrolyzed significantly slower than PC. The rate of PC hydrolysis was measured in the previous work. The hydrolysis of PE in PC-vesicules was followed with use of Victoria blue R dye method. It was shown that the rate of PE hydrolysis in the vesicules is similar to that of PC.
Selishcheva
A A
AA
Miroshnikova
T A
TA
Voronin
M V
MV
Vasilenko
I A
IA
D001409:Bacillus cereus / Q000201:enzymology*; D006868:Hydrolysis; D007700:Kinetics; D008081:Liposomes; D009682:Magnetic Resonance Spectroscopy; D008823:Micelles; D010713:Phosphatidylcholines / Q000378:metabolism*; D010714:Phosphatidylethanolamines / Q000378:metabolism*; D010738:Type C Phospholipases / Q000378:metabolism*; D014867:Water / Q000737:chemistry
D008081:Liposomes; D008823:Micelles; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D014867:Water; D010738:Type C Phospholipases
Osobennosti kinetiki gidroliza fosfolipidov fosfolipazoĭ C iz Bacilluscereus. Gidroliz fosfatidilétanolamina v razlichnykh agregatnykh sostoianiiakh.