title: Purification of a 28 kD non-aggregating tryptic peptide of the Duffy blood group protein.
abstract: Duffy blood group antigenic epitopes are located on a 35-43 kD integral membrane protein of the erythrocyte membrane. This protein functions as a receptor for the human malaria parasite, Plasmodium vivax. The Duffy protein has been difficult to purify because of its tendency to form aggregates. Here we describe purification of a 28 kD tryptic fragment of the Duffy protein and purification of an 18 kD de-glycosylated form of the Duffy tryptic peptide using Thiopropyl Sepharose 6B chromatography and preparative SDS-PAGE. These Duffy-reactive peptides do not form aggregates and may prove amendable to protein sequencing.
authors: University of Louisville School of Medicine, KY.
authors: K
authors: K
authors: Wasniowska
authors: P
authors: P
authors: Eichenberger
authors: F
authors: F
authors: Kugele
authors: T J
authors: TJ
authors: Hadley
mesh_terms: D002478:Cells, Cultured; D002850:Chromatography, Gel; D004375:Duffy Blood-Group System / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D006031:Glycosylation; D006801:Humans; D010446:Peptide Fragments / Q000302:isolation & purification* / Q000378:metabolism; D014357:Trypsin
chemical_list: D004375:Duffy Blood-Group System; D010446:Peptide Fragments; D014357:Trypsin