title: Determination of total polyamines in tumor cells by high-performance capillary zone electrophoresis with indirect photometric detection.
abstract: Tumor cells contain much higher levels of common polyamines such as putrescine, spermidine, and spermine than normal cells. Our previous work demonstrated that high-performance capillary zone electrophoresis with indirect photometric detection was suitable for the determination of underivatized free polyamines in tumor cells. We now report improved sensitivity (7-fold), greater quantitative reproducibility, and quantitation of both bound and free, underivatized polyamines in PC 12 tumor cells. 1,7-Diaminoheptane was used as an internal standard for quantification. Femtomoles of three polyamines and simple cations such as K+ and Na+ were easily separated in less than 10 min. The quantitations for total spermidine and spermine (bound and free) were found to be 2-fold higher than the quantitation for free, unbound polyamines using our previous method. The signal response was linear over 2 orders of magnitude. The method described here provides a fast and simple technique for quantitation of bound, unbound, and total polyamines for use in the study of the biological role of polyamines.
authors: Division of Science, Northeast Missouri State University, Kirksville 63501.
authors: R
authors: R
authors: Zhang
authors: C L
authors: CL
authors: Cooper
authors: Y
authors: Y
authors: Ma
mesh_terms: D000310:Adrenal Gland Neoplasms / Q000737:chemistry*; D000818:Animals; D003959:Diamines / Q000737:chemistry; D004586:Electrophoresis / Q000379:methods*; D016716:PC12 Cells; D010673:Pheochromocytoma / Q000737:chemistry*; D011073:Polyamines / Q000032:analysis*; D011700:Putrescine / Q000032:analysis; D051381:Rats; D013095:Spermidine / Q000032:analysis; D013096:Spermine / Q000032:analysis
chemical_list: D003959:Diamines; D011073:Polyamines; D013096:Spermine; C015127:1,7-diaminoheptane; D013095:Spermidine; D011700:Putrescine