Accumulation of factor F395 in nifNE mutants of Klebsiella pneumoniae.
The nifNE gene products of Klebsiella pneumoniae are required for the in vivo and in vitro synthesis of the iron-molybdenum cofactor (FeMo-co) of nitrogenase. Derepression of nifNE mutants for nitrogenase resulted in the accumulation of a small molecule, factor F395. Factor F395 is protein associated in vivo. We report here initial spectral characterization of this factor.
Downs
D M
DM
Department of Biochemistry, University of Wisconsin, Madison 53706.
Ludden
P W
PW
Shah
V K
VK
D001685:Biological Factors / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D003067:Coenzymes; D004794:Enzyme Repression; D005798:Genes, Bacterial; D007711:Klebsiella pneumoniae / Q000235:genetics / Q000378:metabolism*; D008667:Metalloproteins / Q000378:metabolism*; D000090006:Molybdenum Cofactors; D009154:Mutation; D009586:Nitrogen Fixation / Q000235:genetics; D009591:Nitrogenase / Q000378:metabolism*; D011621:Pteridines / Q000378:metabolism*; D013045:Species Specificity
D001685:Biological Factors; D003067:Coenzymes; D008667:Metalloproteins; D000090006:Molybdenum Cofactors; D009591:Nitrogenase; D011621:Pteridines; C022261:molybdopterin