title: Development of recombinant viral insecticides by expression of an insect-specific toxin and insect-specific enzyme in nuclear polyhedrosis viruses.
abstract: As supplements to classical chemical insecticides, two approaches to develop recombinant baculovirus insecticides are described. In one approach an insect-specific toxin is expressed leading to a dramatic reduction in time to death. In the second approach an insect juvenile hormone esterase is expressed which leads to a reduction in feeding. Modifications of the wildtype esterase led to viruses which reduced the time to death as effectively as did the toxin-expressing virus. In both cases existing recombinant viruses are viewed as leads, and approaches to further improvement in the engineered viruses are suggested. Many of these approaches are based on analogy with the development of classical synthetic insecticides. Using these viruses as examples, the potential utility and limitations of recombinant viruses and other biological insecticides are discussed.
authors: Department of Entomology, University of California, Davis 95616.
authors: B D
authors: BD
authors: Hammock
authors: B F
authors: BF
authors: McCutchen
authors: J
authors: J
authors: Beetham
authors: P V
authors: PV
authors: Choudary
authors: E
authors: E
authors: Fowler
authors: R
authors: R
authors: Ichinose
authors: V K
authors: VK
authors: Ward
authors: J M
authors: JM
authors: Vickers
authors: B C
authors: BC
authors: Bonning
authors: L G
authors: LG
authors: Harshman
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D016367:Baculoviridae / Q000235:genetics; D002265:Carboxylic Ester Hydrolases / Q000235:genetics*; D015870:Gene Expression; D005822:Genetic Vectors; D007301:Insect Hormones / Q000378:metabolism; D007306:Insecticides / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D009498:Neurotoxins / Q000235:genetics*; D010572:Pest Control, Biological; D011994:Recombinant Proteins / Q000235:genetics
chemical_list: D007301:Insect Hormones; D007306:Insecticides; D009498:Neurotoxins; D011994:Recombinant Proteins; D002265:Carboxylic Ester Hydrolases; C038003:juvenile hormone esterase