A set of STS assays targeting the chromosome 22 physical framework markers.
The widespread use of the sequence-tagged site (STS) as a quick, efficient, and reproducible assay for comparing physical and genetic map information promises to facilitate greatly long-range goals of the mapping of the human genome. We have designed 21 STS assays for loci on human chromosome 22. These assays primarily tag the physical framework markers of the long arm of 22, but additional assays have been designed from known genes and loci in the neurofibromatosis 2 (NF2) region. The availability of these assays will make these loci available to the research community without physical transfer of materials and will serve as start points for further efforts to physically map chromosome 22 with yeast artificial chromosome clones.
MacCollin
M
M
Molecular Neurogenetics Laboratory, Massachusetts General Hospital, Charlestown 02129.
Romano
D
D
Budarf
M
M
Denny
C
C
Trofatter
J
J
Menon
A
A
Rouleau
G
G
Fontaine
B
B
Emanuel
B
B
Gusella
J
J
D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D002892:Chromosomes, Human, Pair 22; D004277:DNA, Single-Stranded; D016515:Genes, Neurofibromatosis 2; D005819:Genetic Markers; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D016324:Sequence Tagged Sites
D004277:DNA, Single-Stranded; D005819:Genetic Markers