The natural history of congenital diaphragmatic hernia and pulmonary hypoplasia in the embryo.
Up to now, descriptions of the natural history of congenital diaphragmatic hernia (CDH) associated with pulmonary hypoplasia (PH) are based exclusively on observations made in the fetal period. However, nothing is known about the events that take place in an embryo with CDH. Recently, an animal model of CDH and PH has been established in rat embryos to study the embryology and natural history of this lesion. We exposed 36 pregnant Sprague-Dawley rats to a single dose of 100 mg nitrofen on day 11 of pregnancy. A total of 356 staged embryos and fetuses from day 13 to day 21 were studied by light and scanning electronmicroscopy. The litters of 9 untreated rats (124 normal age-matched embryos and fetuses) served as controls. The abnormal development of the diaphragmatic anlage was first seen in embryos aged 13 to 14 days. A defect appeared in the dorsal part of the diaphragm, normally on the right side. The liver grew through this defect early on. Gut was found in an intrathoracic position only in the very late stages (day 21/22) and newborns. Compared to controls, lungs of nitrofen-embryos with CDH were smaller, depending on the size of liver found in the thoracic cavity. Histologically, compression of lung was absent at these stages. Most authors speculate that CDH results because the pleuroperitoneal canals fail to close at the end of the embryonic period (ie, week 8 to 10 in human development) leading to a defect in the dorsolateral region of the diaphragm. However, contradictory to this assumption, our findings indicate that diaphragmatic defects develop in early embryonic life.(ABSTRACT TRUNCATED AT 250 WORDS)
Kluth
D
D
Department of Pediatric Surgery, University Hospital, Hamburg, Germany.
Tenbrinck
R
R
von Ekesparre
M
M
Kangah
R
R
Reich
P
P
Brandsma
A
A
Tibboel
D
D
Lambrecht
W
W
D000818:Animals; D005314:Embryonic and Fetal Development; D005260:Female; D005333:Fetus / Q000002:abnormalities / Q000473:pathology; D006548:Hernia, Diaphragmatic / Q000196:embryology* / Q000473:pathology; D065630:Hernias, Diaphragmatic, Congenital; D008168:Lung / Q000002:abnormalities* / Q000196:embryology / Q000473:pathology / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D008954:Models, Biological; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley