title: Levels of five mycotoxins in grains harvested in Atlantic Canada as measured by high performance liquid chromatography.
abstract: An analytical protocol using high performance liquid chromatography (HPLC) was used to analyze samples of spring wheat, winter wheat, 2-row barley, and 6-row barley over a period of three years for the presence of five mycotoxins. These included deoxynivalenol, zearalenone, T-2 toxin, HT-2 toxin, and diacetoxyscirpenol. The protocol employed a single extraction step using acetonitrile-water and two cleanup procedures. One utilized a solid-phase extraction column and the other a charcoal-alumina column. Detection limits ranged from 0.02 to 0.15 micrograms of mycotoxin g-1 grain. Little T-2 toxin, HT-2 toxin, or diacetoxyscirpenol was found in the samples. Deoxynivalenol was detected in 53 to 62% of the samples tested and zearalenone in 25-29% of the samples. Several enzyme-linked immunosorbent assays were used for comparison purposes with deoxynivalenol, zearalenone, and T-2 toxin. These kits provided reliable qualitative, but not quantitative, data.
authors: Department of Biology, Nova Scotia Agricultural College, Truro, Canada.
authors: G W
authors: GW
authors: Stratton
authors: A R
authors: AR
authors: Robinson
authors: H C
authors: HC
authors: Smith
authors: L
authors: L
authors: Kittilsen
authors: M
authors: M
authors: Barbour
mesh_terms: D002170:Canada; D002851:Chromatography, High Pressure Liquid; D002523:Edible Grain / Q000737:chemistry*; D005506:Food Contamination / Q000032:analysis*; D009183:Mycotoxins / Q000032:analysis*
chemical_list: D009183:Mycotoxins