Occurrence and purification of the photoactive yellow protein of Ectothiorhodospira halophila (PYP) and of immunologically related proteins of Rhodospirillum salexigens and Chromatium salexigens and intracellular localization of PYP.
The photoactive yellow protein of Ectothiohodospira halophila (PYP) was purified to homogeneity by an advanced method and applied as an affinity ligand for the isolation of an anti-PYP IgG fraction which was used for immunoscreening. The distribution of proteins immunologically related to PYP was investigated in protein fractions of 51 strains from 38 species of non-halophilic and halophilic phototrophic and chemotrophic eubacteria and archaeobacteria. Strong immunoreactive bands indicating the presence of authentic PYP on Western blots (apparent mass 17.8 kDa) was only found in the strains of E. halophila. Additionally, two soluble proteins of Chromatium salexigens and Rhodospirillum salexigens (apparent molecular masses 16.4 and 19 kDa, respectively) cross-reacted to approx. 6% and 4%. Analyses of cell fractions of E. halophila revealed that PYP is a cytoplasmic protein.
Thiemann
B
B
Institut für Mikrobiologie und Biotechnologie der Rheinischen Friedrich-Wilhelms-Universität, Bonn, Germany.
Imhoff
J F
JF
D000907:Antibodies, Bacterial; D001426:Bacterial Proteins / Q000276:immunology / Q000302:isolation & purification*; D015153:Blotting, Western; D002451:Cell Compartmentation; D002841:Chromatiaceae / Q000737:chemistry* / Q000276:immunology; D002844:Chromatium / Q000737:chemistry; D002845:Chromatography / Q000379:methods; D003429:Cross Reactions; D020283:Photoreceptors, Microbial; D012246:Rhodospirillum / Q000737:chemistry* / Q000276:immunology; D013045:Species Specificity; D013347:Subcellular Fractions / Q000737:chemistry
D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D020283:Photoreceptors, Microbial; C050162:photoactive yellow protein, Bacteria