Preliminary histoenzymatic muscular evaluations after ischemia-reperfusion damage.
The aim of this study was to evaluate the muscular regenerative properties after an induced ischemia/reperfusion injury of 4 hours and 30 min. of the left hind limb of the rat and to evaluate and compare the efficacy of native and modified polyethylene glycol superoxide dismutase (SOD and mPEG-SOD). In particular, we try to find a suitable method to study the histological and histoenzymatic properties of muscular fibres, focusing also the technical procedure employed to evaluate the new activated motor end-plates. Biopsies from flexor digitorum superficialis were collected immediately after the ischemia/reperfusion damage and also at 4th, 6th and 10th days. The adopted protocol showed a good reliability both for the necrosis and the regenerative process evaluations. However the assessment of regenerative and reinnervative processes will be completed by histomorphometric analysis.
Servizio di Chirurgia Sperimentale, Istituto di Ricerca Codivilla-Putti, Istituti Ortopedici Rizzoli, Bologna.
S
S
Capelli
M
M
Rocca
L
L
Orienti
G
G
Giavaresi
R
R
Giardino
D000818:Animals; D003131:Combined Modality Therapy; D005263:Femoral Artery / Q000601:surgery; D006614:Hindlimb / Q000098:blood supply / Q000601:surgery; D007511:Ischemia / Q000188:drug therapy* / Q000201:enzymology / Q000473:pathology; D009045:Motor Endplate / Q000502:physiology / Q000648:ultrastructure; D018482:Muscle, Skeletal / Q000201:enzymology / Q000473:pathology*; D009336:Necrosis; D011092:Polyethylene Glycols / Q000008:administration & dosage; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species; D012038:Regeneration; D015427:Reperfusion Injury / Q000201:enzymology / Q000473:pathology / Q000517:prevention & control*; D013482:Superoxide Dismutase / Q000008:administration & dosage / Q000627:therapeutic use*
D017382:Reactive Oxygen Species; D011092:Polyethylene Glycols; D013482:Superoxide Dismutase