Preliminary histoenzymatic muscular evaluations after ischemia-reperfusion damage.
The aim of this study was to evaluate the muscular regenerative properties after an induced ischemia/reperfusion injury of 4 hours and 30 min. of the left hind limb of the rat and to evaluate and compare the efficacy of native and modified polyethylene glycol superoxide dismutase (SOD and mPEG-SOD). In particular, we try to find a suitable method to study the histological and histoenzymatic properties of muscular fibres, focusing also the technical procedure employed to evaluate the new activated motor end-plates. Biopsies from flexor digitorum superficialis were collected immediately after the ischemia/reperfusion damage and also at 4th, 6th and 10th days. The adopted protocol showed a good reliability both for the necrosis and the regenerative process evaluations. However the assessment of regenerative and reinnervative processes will be completed by histomorphometric analysis.
Capelli
S
S
Servizio di Chirurgia Sperimentale, Istituto di Ricerca Codivilla-Putti, Istituti Ortopedici Rizzoli, Bologna.
Rocca
M
M
Orienti
L
L
Giavaresi
G
G
Giardino
R
R
D000818:Animals; D003131:Combined Modality Therapy; D005263:Femoral Artery / Q000601:surgery; D006614:Hindlimb / Q000098:blood supply / Q000601:surgery; D007511:Ischemia / Q000188:drug therapy* / Q000201:enzymology / Q000473:pathology; D009045:Motor Endplate / Q000502:physiology / Q000648:ultrastructure; D018482:Muscle, Skeletal / Q000201:enzymology / Q000473:pathology*; D009336:Necrosis; D011092:Polyethylene Glycols / Q000008:administration & dosage; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species; D012038:Regeneration; D015427:Reperfusion Injury / Q000201:enzymology / Q000473:pathology / Q000517:prevention & control*; D013482:Superoxide Dismutase / Q000008:administration & dosage / Q000627:therapeutic use*
D017382:Reactive Oxygen Species; D011092:Polyethylene Glycols; D013482:Superoxide Dismutase