Developmental regulation of protein kinase-A and -C activities in the baboon fetal adrenal.
We have previously demonstrated that the estrogen-regulated change in transuteroplacental metabolism of cortisol (F) and cortisone (E) from preferential reduction (E to F) at midgestation to oxidation (F to E) near term results in activation of the hypothalamic-pituitary-adrenal axis of the baboon and the ontogenesis of rate-limiting steroidogenic enzymes, culminating in de novo F secretion. It is well established that transcription of messages activated by peptide-mediated binding to membrane receptors can occur via cAMP-dependent protein kinase-A (PKA) and/or phospholipid/calcium-dependent protein kinase-C (PKC). The present study was designed to determine whether basal levels of PKA and PKC in the fetal adrenal are developmentally regulated during baboon gestation and, thus, could provide the mechanism(s) by which activation of the fetal adrenal near term is mediated. Fetal adrenal glands were obtained on day 100 (n = 8) and day 165 (n = 6) of gestation (term = day 184) from untreated baboons and on day 100 after treatment of the mother with estradiol benzoate, injected sc between days 70-100 to increase estrogen production. PKA activity (picomoles of 32P incorporated into kemptide per min/mg protein) was determined by incubation of adrenal cytosol (12,000 x g; 0.3-30 micrograms protein) in reaction mixtures containing 0.25 mM ATP, 1 x 10(6) dpm [lambda-32P]ATP, and 3 micrograms kemptide in the presence or absence of 0.02 mM cAMP. PKC activity (picomoles of 32P incorporated into histone IIIS per min/mg protein) was determined in cytosol (105,000 x g) and detergent-solubilized membrane fractions after incubation with 0.02 mM ATP, 50 micrograms histone IIIS, and 1 x 10(6) dpm [lambda-32P]ATP in the presence or absence of calcium and phospholipids. Mean (+/- SE) maternal serum estradiol concentrations (nanograms per ml) were 3-fold greater (P < 0.05) at term (1.9 +/- 0.3) than at midgestation and increased (P < 0.05) after treatment with estradiol. PKA activity was greater at term (3965 +/- 546) than at midgestation (2130 +/- 240) and increased (P < 0.05) 2-fold after treatment with estrogen (3525 +/- 416) at midgestation. PKC activity was always 3- to 4-fold lower than that of PKA and was similar in the cytosol and membrane fractions of the cell. In contrast to PKA, cytosolic PKC activity was similar at mid (265 +/- 98)-and late (353 +/- 99) gestation and was not altered by treatment with estradiol (223 +/- 27).(ABSTRACT TRUNCATED AT 400 WORDS)
Department of Physiology, Eastern Virginia Medical School, Norfolk, Virginia 23501.
W A
WA
Davies
K A
KA
Berghorn
E D
ED
Albrecht
G J
GJ
Pepe
D000311:Adrenal Glands / Q000196:embryology*; D000735:Androstenedione / Q000494:pharmacology; D000818:Animals; D002848:Chromatography, DEAE-Cellulose; D004305:Dose-Response Relationship, Drug; D005314:Embryonic and Fetal Development; D004958:Estradiol / Q000097:blood / Q000494:pharmacology; D005260:Female; D005333:Fetus / Q000378:metabolism*; D007527:Isoenzymes / Q000378:metabolism; D010215:Papio / Q000196:embryology*; D011247:Pregnancy; D011493:Protein Kinase C / Q000378:metabolism*; D011494:Protein Kinases / Q000737:chemistry / Q000378:metabolism*
D007527:Isoenzymes; D000735:Androstenedione; D004958:Estradiol; D011494:Protein Kinases; D011493:Protein Kinase C