[Early changes of sugar chains in bladder luminal cell membrane of rat treated with N-butyl-N-(4-hydroxybutyl) nitrosamine detected by immunohistochemical transmission electron microscopy].
Early changes of sugar chain detected by binding with lectins on luminal cell membrane of rat bladder epithelium treated with N-butyl-N-(4-hydroxybutyl) nitrosamine (BBN) were immunohistochemically examined with a transmission electron microscopy using ferritin-labeling lectins. BBN was given to male Wistar rats at the concentration of 0.05 W/V% in drinking water, and the rats were sacrificed at 4, 8, 16, 20 weeks, respectively, after the beginning of the BBN treatment. The bladder was treated with Half Karnovsky's solution via intra aortic cannulation for 20 minutes, and then removed. A part of bladder was examined grossly and by a light microscopy. The other part was cut into 3 x 3 mm squares and fixed with 2.5% glutaraldehyde solution and washed with 0.1 M cacogyl buffer with 10% sucrose. The tissue was sectioned and then incubated with ferritin-labeling lectins; concanavalin A (Con A), ulex europeaus agglutinin (UEA-1), peanut agglutinin (PNA) and dolichos biflorus agglutinin (OBA), respectively. These specimens were examined under a transmission electron microscopy. In cell membrane of the normal bladder, binding of Con A was found occasionally and binding of PNA was found focally, but binding of UEA-1 and DBA occurred incompletely on the cell membrane. The plaque and ridge on cell surface of bladder epithelium found to be obscured at 8 weeks after the treatment with BBN, and microvilli were barely observed under an electron microscope, but they increased gradually and could be seen by the microscope at the time of 16 weeks after the BBN treatment.(ABSTRACT TRUNCATED AT 250 WORDS)
Matsuoka
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Department of Urology, Kagawa Medical School, Japan.
D000818:Animals; D002085:Butylhydroxybutylnitrosamine; D002295:Carcinoma, Transitional Cell / Q000139:chemically induced / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism / Q000648:ultrastructure; D004848:Epithelium / Q000378:metabolism; D006023:Glycoproteins / Q000378:metabolism*; D007150:Immunohistochemistry; D037102:Lectins / Q000378:metabolism*; D008297:Male; D008854:Microscopy, Electron; D051381:Rats; D017208:Rats, Wistar; D001743:Urinary Bladder / Q000378:metabolism* / Q000648:ultrastructure; D001749:Urinary Bladder Neoplasms / Q000139:chemically induced / Q000378:metabolism*
D006023:Glycoproteins; D037102:Lectins; D002085:Butylhydroxybutylnitrosamine