Isolation and characterization of the IS3-like element from Thermus aquaticus.
We have cloned and characterized a genetic element (1187 bp) that is responsible for the induction of thermotolerance as well as ompC expression in E. coli. This element (ISLtaq1) was isolated from Thermus aquaticus. DNA and protein data bases were searched with this element (ISLtaq1), which suggested it to be very similar to IS150 belonging to the IS3 family. ORF1, found on ISLtaq1, which encodes 100 amino acids (aa), had a DNA-binding motif: a helix-turn-helix and a leucine zipper. In fact, when the ORF1 protein was overproduced in E. coli, thermotolerance as well as ompC expression was induced.
Department of Agricultural Chemistry, Kinki University, Nara, Japan.
R
R
Utsumi
M
M
Ikeda
T
T
Horie
M
M
Yamamoto
A
A
Ichihara
Y
Y
Taniguchi
R
R
Hashimoto
H
H
Tanabe
K
K
Obata
M
M
Noda
D000220:Adaptation, Biological; D000595:Amino Acid Sequence; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods; D004251:DNA Transposable Elements / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics / Q000502:physiology; D029968:Escherichia coli Proteins; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D016153:Genes, Suppressor; D006358:Hot Temperature; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes; D009154:Mutation; D013824:Thermus / Q000737:chemistry / Q000235:genetics* / Q000502:physiology
D001425:Bacterial Outer Membrane Proteins; D004251:DNA Transposable Elements; D029968:Escherichia coli Proteins; D009097:Multienzyme Complexes; C057843:envZ protein, E coli