B-cell chronic lymphocytic leukaemia populations respond stochastically to combinations of growth signals in vitro.
The ability of B-CLL lymphocyte populations to respond to cytokine stimulation of mononuclear cultures was examined using 3-colour immunofluorescence and multiparameter flow cytometry. The simultaneous detection of the membrane antigens CD5 and CD19, with the proliferation antigen Ki-67, allowed examination of the cell-cycle entry of the B-CLL lymphocyte population. Upon stimulation with IFN-gamma, IFN-gamma+IL-2, TPA+IL-4, between 10 and 44% of B-CLL lymphocytes from 10 patients were stimulated to enter into cell-cycle. This B-CLL response was notably more homogenous than the non-clonal lymphocyte response. Except in the case of IFN-gamma alone, T-cell response did not correlate with B-CLL response, suggesting B-CLL lymphocytes respond directly to combinations of growth signals. Considering the B-CLL population as homogenous, B-CLL lymphocytes respond in a stochastic manner.
Department of Haematology, Queens University of Belfast, Royal Victoria Hospital, Northern Ireland.
A P
AP
McManus
Z R
ZR
Desai
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T
Lavabre-Bertrand
D000368:Aged; D001402:B-Lymphocytes / Q000276:immunology; D002478:Cells, Cultured; D002999:Clone Cells; D005260:Female; D006801:Humans; D016130:Immunophenotyping; D007371:Interferon-gamma / Q000494:pharmacology; D007376:Interleukin-2 / Q000494:pharmacology; D015847:Interleukin-4 / Q000494:pharmacology; D019394:Ki-67 Antigen; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000097:blood* / Q000276:immunology* / Q000473:pathology; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000276:immunology* / Q000473:pathology; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000097:blood; D009367:Neoplasm Staging; D009687:Nuclear Proteins / Q000097:blood; D013601:T-Lymphocytes / Q000276:immunology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D007376:Interleukin-2; D019394:Ki-67 Antigen; D009363:Neoplasm Proteins; D009687:Nuclear Proteins; D015847:Interleukin-4; D007371:Interferon-gamma; D013755:Tetradecanoylphorbol Acetate