Enhancement of in vitro antibody production of murine splenocytes by ascorbic acid 2-O-alpha-glucoside.
Using an antigen-specific plaque forming cell (PFC) assay, we have studied the effect of ascorbic acid 2-O-alpha-glucoside (AA-2G), a new stable derivative of ascorbic acid (AsA), on antibody production in cultured murine splenocytes in comparison with that of AsA. A single addition of AA-2G (0.0625-1.0 mM) remarkably stimulated both anti-SRBC (sheep red blood cell, TD antigen) and anti-TNP (trinitrophenyl, TI antigen) PFC responses in a dose-dependent manner, although AsA failed to stimulate their responses. However, repeated additions of AsA at 12-h intervals during the cultivation resulted in enhancement of the anti-SRBC PFC response, and the magnitude of stimulation was comparable to that obtained by a single addition of AA-2G. AA-2G's effect was abrogated in the presence of castanospermine (alpha-glucosidase inhibitor) in the medium, indicating that the immunostimulation brought about by AA-2G is attributed to AsA released from the glucoside by alpha-glucosidase of cultured cells. In fact, the cells maintained a certain amount of AsA for a relatively long time after exposure to AA-2G. In contrast, AsA levels in the cells treated with AsA quickly decreased. AA-2G markedly enhanced lipopolysaccharide(LPS)-induced proliferative response, and could affect the concanavalin A (Con A) response weakly. These results suggest that AsA effectively potentiates B-cell functions in the humoral immune system. Thus, we conclude that AA-2G is capable of enhancing antibody production in the cultured splenocytes via a continuous supplementation of AsA and that AA-2G is available for long-term cell cultures as a useful tool for analyzing biological function of AsA.
Department of Immunochemistry, Faculty of Pharmaceutical Sciences, Okayama University, Japan.
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Yamamoto
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Tanaka
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Muto
D000818:Animals; D000917:Antibody Formation / Q000187:drug effects*; D001205:Ascorbic Acid / Q000031:analogs & derivatives* / Q000032:analysis / Q000494:pharmacology; D002478:Cells, Cultured; D004912:Erythrocytes / Q000276:immunology; D005260:Female; D007212:Indolizines / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D013154:Spleen / Q000166:cytology / Q000276:immunology*; D054836:Indolizidines
D001205:Ascorbic Acid; D007212:Indolizines; C065378:ascorbic acid 2-O-glucoside; C037806:castanospermine; D054836:Indolizidines