Release of the outer membrane vesicles from Vibrio cholerae and Vibrio parahaemolyticus.
We found numerous small vesicles released from the cell by thin sectioning of the plate culture of Vibrio cholerae and V. parahaemolyticus fixed with the freeze-substitution technique. From the broth media of exponentially growing bacteria we could collect the vesicles by the centrifugation but not enough without fixation. The vesicles are encompassed with a membrane structure similar to the outer membrane of these bacteria. The anti-O (Inaba) serum reacted with the surface of the vesicles and the inside of the vesicle are generally filled with an electron-dense mass.
Department of Bacteriology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
K
K
Kondo
A
A
Takade
K
K
Amako
D001426:Bacterial Proteins / Q000032:analysis; D002462:Cell Membrane / Q000648:ultrastructure; D004591:Electrophoresis, Polyacrylamide Gel; D008854:Microscopy, Electron; D015388:Organelles / Q000737:chemistry / Q000648:ultrastructure; D014734:Vibrio cholerae / Q000648:ultrastructure*; D014736:Vibrio parahaemolyticus / Q000648:ultrastructure*
D001426:Bacterial Proteins