Chloramphenicol, an inhibitor of mitochondrial protein synthesis, inhibits myoblast fusion and myotube differentiation.
Chloramphenicol (100 micrograms/ml), an inhibitor of mitochondrial protein synthesis, inhibits the fusion of myoblasts isolated from chick embryo pectoral muscle in both, serum supplemented and serum-free culture conditions. This inhibition of myotube formation appeared reversible only in the defined media. Tryptose phosphate broth and nucleosides, when added in the presence of chloramphenicol, were able to restore the cell capacity to proliferate but not to fuse and differentiate.
Department of Cell Biology, Jan Zurzycki Institute of Molecular Biology, Jagiellonian University, Kraków, Poland.
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Korohoda
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Pietrzkowski
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Reiss
D000818:Animals; D001769:Blood; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002459:Cell Fusion / Q000187:drug effects; D002478:Cells, Cultured; D002642:Chick Embryo; D002701:Chloramphenicol / Q000494:pharmacology*; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism*; D009132:Muscles / Q000166:cytology* / Q000196:embryology; D014176:Protein Biosynthesis; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology*
D011500:Protein Synthesis Inhibitors; D002701:Chloramphenicol