Control of intracellular growth of Mycobacterium fortuitum by human monocytes in vitro.
The ability of human monocytes to phagocytize and respond to infection by Mycobacterium fortuitum was tested using the method of Crowle and May. The monocytes were obtained from heparinized donor blood samples and separated from lymphocytes by adherence to plastic surfaces. M. fortuitum infection was performed immediately. Intracellular viability was indicated by colony forming units (CFU) done at 2 hour intervals. Monocytes were found to cause a rapid reduction in CFU during the first 2 hours of incubation. The rate of killing of M. fortuitum decreased thereafter but continued for the entire 8-hour study period. In parallel tests, ciprofloxacin and ofloxacin were added to the culture medium. Increased intracellular killing was observed with drug concentrations above 2 x MIC in both cases. Ofloxacin showed better antimycobacterial effects than ciprofloxacin.
Infektiologie, Zentrum der Innere Medizin, Klinikum der J.W. Goethe Universitaet, Frankfurt am Main, Germany.
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Nziramasanga
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Shah
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Stille
D002939:Ciprofloxacin / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D008826:Microbial Sensitivity Tests; D009000:Monocytes / Q000187:drug effects / Q000382:microbiology* / Q000502:physiology; D009161:Mycobacterium / Q000187:drug effects / Q000254:growth & development*; D015242:Ofloxacin / Q000494:pharmacology; D010587:Phagocytosis / Q000187:drug effects
D002939:Ciprofloxacin; D015242:Ofloxacin