Isolation of 353 NotI-linking clones and 62 DNA markers (DXS607-DXS668) from human chromosome Xq24-->qter.
Through restriction enzyme digestion of 1,784 cosmid DNAs derived from human chromosome region Xq24-->qter, we identified 353 NotI-linking cosmids (11 individual groups of overlapping NotI-linking cosmids at Xq24-->q25.6 groups at Xq26-->q27, and 19 groups at Xq28). Sixty-two of the 353 clones (DXS607-DXS668) contained multiple rare-cutter sites, including multiple SacII sites within a 2-kb region, as well as one to six sites for BssHII, MluI, NotI, or NruI.
Department of Neurology, Tokyo Medical and Dental University, Japan.
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Kaneko
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Warren
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Miyatake
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Tsuji
D003360:Cosmids; D015252:Deoxyribonucleases, Type II Site-Specific; D005819:Genetic Markers; D006801:Humans; D015183:Restriction Mapping; D014960:X Chromosome
D005819:Genetic Markers; D015252:Deoxyribonucleases, Type II Site-Specific; C038565:GCGGCCGC-specific type II deoxyribonucleases