Differences between natural and recombinant interleukin-2 revealed by gel electrophoresis and capillary electrophoresis.
High-performance capillary electrophoresis (HPCE) is shown to be useful for analysis of interleukin-2 (IL-2) in its native state under non-reducing conditions. The results obtained were compared with those from analysis of IL-2 by protein blotting after sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE) under denaturing and reducing conditions. In addition, resolution of the different glycosylated and non-glycosylated natural IL-2 species was achieved by HPCE. The HPCE electropherogram of native IL-2 could easily generate quantitative amounts of the different naturally occurring IL-2 species. For HPCE of IL-2 run times of less than 10 min are sufficient, and only extremely small amounts of IL-2 are needed. In this report, human IL-2 expressed in bacteria has been analysed by HPCE and the existence of two recombinant IL-2 forms was demonstrated.
Knüver-Hopf
J
J
German Red Cross Blood Transfusion Center of Lower Saxony, Springe, Germany.
Mohr
H
H
D019075:Electrophoresis, Capillary / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D006801:Humans; D007376:Interleukin-2 / Q000737:chemistry*; D011994:Recombinant Proteins / Q000737:chemistry
D007376:Interleukin-2; D011994:Recombinant Proteins