Mapping of multiple mouse loci related to the farnesyl pyrophosphate synthetase gene.
The prenyltransferases are a class of enzymes involved in the synthesis of sterol and nonsterol isoprene compounds. We report here the chromosomal mapping of nine loci in the mouse that hybridize to the cDNA for the enzyme farnesyl pyrophosphate synthetase (FPS), a prenyltransferase that catalyzes the synthesis of an intermediate common to both the sterol and nonsterol branches of the isoprene biosynthetic pathway. Mapping was performed with genomic DNA from a mouse-hamster somatic cell hybrid panel, and by linkage analysis with recombinant inbred strains and the progeny of an interspecific backcross. The mapped loci have been designated farnesyl pyrophosphate synthetase-like-1 (Fpsl-1) on mouse Chromosome (Chr) 3; Fpsl-2 on Chr 4; Fpsl-3, Fpsl-4, and Fpsl-5, dispersed on Chr 10; Fpsl-6 on Chr 12; Fpsl-7 on Chr 13; Fpsl-8 on Chr 17; and Fpsl-9 on Chr X. It is presently unclear which of these loci encode active prenyltransferases and which may correspond to pseudogenes. The strongly hybridizing loci provide convenient genetic markers for seven mouse chromosomes.
Department of Medicine, University of California, Los Angeles 90024.
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Andalibi
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Diep
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Quon
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Mohandas
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Taylor
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Lusis
D000818:Animals; D002874:Chromosome Mapping; D006224:Cricetinae; D003433:Crosses, Genetic; D004122:Dimethylallyltranstransferase / Q000235:genetics*; D008040:Genetic Linkage; D005819:Genetic Markers; D006822:Hybrid Cells; D006824:Hybridization, Genetic; D051379:Mice; D008815:Mice, Inbred Strains / Q000235:genetics*; D005810:Multigene Family; D009115:Muridae / Q000235:genetics*; D011110:Polymorphism, Genetic; D011544:Pseudogenes; D011995:Recombination, Genetic
D005819:Genetic Markers; D004122:Dimethylallyltranstransferase