Quantitation of benzoylnorecgonine and other cocaine metabolites in meconium by high-performance liquid chromatography.
A method for simultaneous extraction of cocaine and metabolites benzoylnorecgonine, benzoylecgonine and norcocaine from meconium was developed. The procedure uses solid-phase extraction columns with both cation-exchange and hydrophobic properties after vortex-mixing meconium with methanol. Chromatography utilizes reversed-phase high-performance liquid chromatography with a C18 column and phosphate buffer-acetonitrile as mobile phase. The method is specific and sensitive to 50 ng/g meconium for all compounds. Standard curves are linear from 0.05 to 5.0 micrograms/g (r2 > or = 0.989). Intra-assay coefficients of variation were < or = 6.9%. Meconium from infants exposed to cocaine in utero contained varying combinations of the four drugs.
Department of Pharmacology, School of Medicine, Oregon Health Sciences University, Portland 97201-3098.
L J
LJ
Murphey
G D
GD
Olsen
R J
RJ
Konkol
D002851:Chromatography, High Pressure Liquid; D003042:Cocaine / Q000031:analogs & derivatives* / Q000032:analysis; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D008470:Meconium / Q000737:chemistry*; D011247:Pregnancy; D019966:Substance-Related Disorders / Q000378:metabolism
C008671:norcocaine; C005618:benzoylecgonine; D003042:Cocaine; C012209:benzoylnorecgonine