High-level production of biologically active human cytosolic phospholipase A2 in baculovirus-infected insect cells.
Infection of Spodoptera frugiperda insect cells with a recombinant baculovirus expressing human cytosolic phospholipase A2 (cPLA2) resulted in the production of biologically active protein. The level of recombinant human cPLA2 production in infected insect cells was at least 50-fold higher than that observed in human monoblast U937 cells.
Department of Gene Expression Sciences, SmithKline Beecham Pharmaceuticals, King of Prussia, PA 19406-0939.
B Y
BY
Amegadzie
D
D
Jiampetti
R J
RJ
Craig
E
E
Appelbaum
A R
AR
Shatzman
R J
RJ
Mayer
A G
AG
DiLella
D000818:Animals; D016367:Baculoviridae; D003600:Cytosol / Q000201:enzymology; D004274:DNA, Recombinant; D006801:Humans; D009036:Moths; D010741:Phospholipases A / Q000096:biosynthesis / Q000235:genetics*; D054467:Phospholipases A2; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics
D004274:DNA, Recombinant; D011993:Recombinant Fusion Proteins; D010741:Phospholipases A; D054467:Phospholipases A2