Isolation and biochemical characterization of heparin-binding proteins from boar seminal plasma: a dual role for spermadhesins in fertilization.
Sperm surface-coated heparin-binding proteins originating from secretions of the male sexual accessory glands, are known to play a pivotal role as extrinsic regulatory factors during sperm capacitation in many mammalian species. They interact with glycosaminoglycans present in the female genital tract and enhance the subsequent zona pellucida-induced acrosome reaction. We have isolated heparin-binding proteins from boar seminal plasma by affinity chromatography on heparin-Sepharose and reverse-phase HPLC. N-Terminal sequence analysis of these proteins identified a boar counterpart of the bovine capacitation factors BSP-A1/2 (also called PDC-109) and BSP-A3. Several carbohydrate- and zona pellucida-binding proteins, which belong to the newly described spermadhesin family, were also identified as heparin-binding proteins. Our results imply that, besides other capacitation factors, members of the spermadhesin family may play a dual role in sperm capacitation and fertilization in the pig.
Sanz
L
L
Dermatologische Klinik und Poliklinik, UNiversität München, Federal Republic of Germany.
Calvete
J J
JJ
Mann
K
K
Gabius
H J
HJ
Töpfer-Petersen
E
E
D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D002352:Carrier Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000502:physiology; D005260:Female; D005306:Fertilization / Q000502:physiology; D006493:Heparin / Q000378:metabolism* / Q000494:pharmacology; D066298:In Vitro Techniques; D008297:Male; D008969:Molecular Sequence Data; D011134:Polysaccharides / Q000494:pharmacology; D012661:Semen / Q000737:chemistry* / Q000502:physiology; D029589:Seminal Plasma Proteins; D013094:Spermatozoa / Q000378:metabolism; D013552:Swine; D015044:Zona Pellucida / Q000187:drug effects / Q000378:metabolism
D002352:Carrier Proteins; D011134:Polysaccharides; D029589:Seminal Plasma Proteins; C425042:spermadhesin; D006493:Heparin; C007789:fucoidan