The role of penicillin-binding protein 3 (PBP 3) in cefotaxime resistance in Streptococcus pneumoniae.
A pneumococcal strain, with a reduced amount of penicillin-binding protein 3 (PBP 3), permitted an analysis of the role of this protein in cefotaxime resistance. We observed that reduced amounts of PBP 3 sensitize the bacteria to high temperature, to excess glycine and to some D-amino acids. These phenotypes suggest that the amount of PBP 3 may influence the membrane properties of the bacteria. The strain with reduced PBP 3 was transformed to cefotaxime resistance. We show that the PBP 3 mutation, in certain genetic backgrounds, decreases the level of resistance to cefotaxime by a factor of 2. Models are presented to explain this result.
Laboratoire de Microbiologie et Génétique Moléculaire du CNRS, Toulouse, France.
L
L
Selakovitch-Chenu
L
L
Seroude
A M
AM
Sicard
D001426:Bacterial Proteins; D002352:Carrier Proteins; D002439:Cefotaxime / Q000494:pharmacology*; D004352:Drug Resistance, Microbial / Q000235:genetics; D006602:Hexosyltransferases / Q000378:metabolism*; D009097:Multienzyme Complexes / Q000378:metabolism*; D002267:Muramoylpentapeptide Carboxypeptidase; D046915:Penicillin-Binding Proteins; D010458:Peptidyl Transferases / Q000378:metabolism*; D013296:Streptococcus pneumoniae / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D014169:Transformation, Bacterial
D001426:Bacterial Proteins; D002352:Carrier Proteins; D009097:Multienzyme Complexes; D046915:Penicillin-Binding Proteins; D010458:Peptidyl Transferases; D006602:Hexosyltransferases; D002267:Muramoylpentapeptide Carboxypeptidase; D002439:Cefotaxime