[Production of hepatitis B virus from peripheral blood lymphocytes stimulated with phytohemagglutinin].
BACKGROUND
It was studied the production of HBV from lymphocytes from peripheral blood induced with a mitogen agent as phytohemagglutinin (PHA), in patients infected by HIV.


METHODS
The methodology developed included the culture of peripheral blood mononuclear cells and its induction to proliferate with PHA, in order to study the viral activity in the supernatants of the cultures by detection of HIV-Ag (ELISA), HBsAg (ELISA) and DNA-HBV (Dot-blot).


RESULTS
Production of HBV in 23 out of the 42 patients included in this study, corresponding to 21 anti-HIV positive and 2 anti-HIV negative.


CONCLUSIONS
Induction to proliferate lymphocytes with a mitogen agent as PHA may represent an alternative way of culture to study interactions between HIV and HBV.
Departamento de Microbiología e Inmunología, Universidad del País Vasco.
L
L
Sarría
L
L
Gallego
B
B
de las Heras
M
M
Basaras
R
R
Cisterna
D002478:Cells, Cultured; D004279:DNA, Viral / Q000032:analysis; D015658:HIV Infections / Q000150:complications; D006509:Hepatitis B / Q000097:blood / Q000150:complications / Q000382:microbiology; D006514:Hepatitis B Surface Antigens / Q000032:analysis; D006515:Hepatitis B virus / Q000254:growth & development* / Q000302:isolation & purification / Q000502:physiology; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects*; D008214:Lymphocytes / Q000187:drug effects / Q000382:microbiology*; D010835:Phytohemagglutinins / Q000494:pharmacology*; D014775:Virus Activation / Q000187:drug effects*; D014779:Virus Replication / Q000187:drug effects
D004279:DNA, Viral; D006514:Hepatitis B Surface Antigens; D010835:Phytohemagglutinins
Producción de virus de la hepatitis B a partir de linfocitos de sangre periférica estimulados con fitohemaglutinina.