Glycan structure of blood group-A antigen in hamster normal tissues and pancreatic cancers.
Pancreatic cancers induced by N-nitrosobis(2-oxopropyl)amine in Syrian hamsters produce blood group A antigen. The glycan structure of the blood group A antigen-bearing glycoproteins purified from pancreatic cancer cells has been shown previously to be bound to Asn-linked complex oligosaccharides. Because blood group A antigen has usually been described as being on Ser/Thr-linked glycans, the distribution and glycan-protein linkage of the antigen were examined in normal hamster tissues in comparison with the findings on pancreatic cancer cells. The gastrointestinal tract, excluding the small intestine, expressed blood group A antigen. The liver, pancreas, and gallbladder did not show blood group A reactivity. Blood group A antigen in the proximal gastrointestinal tract was resistant to peptide N-glycosidase F digestion, which cleaves Asn-linked glycans from core proteins. Blood group A antigen was peptide N-glycosidase F sensitive in membrane preparations from pancreatic cancers. In the colon, this antigen was only partially removed by peptide-N-glycosidase F. These results demonstrate a difference in the structure of blood group A antigen-associated glycan between pancreatic cancers and normal hamster gastrointestinal tissues.
Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha 68198-4525.
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D000017:ABO Blood-Group System / Q000032:analysis* / Q000737:chemistry; D000818:Animals; D015153:Blotting, Western; D002273:Carcinogens; D002458:Cell Fractionation; D002462:Cell Membrane / Q000737:chemistry / Q000648:ultrastructure; D006224:Cricetinae; D004064:Digestive System / Q000737:chemistry / Q000166:cytology / Q000473:pathology; D004591:Electrophoresis, Polyacrylamide Gel; D007150:Immunohistochemistry; D007425:Intracellular Membranes / Q000737:chemistry / Q000648:ultrastructure; D008647:Mesocricetus; D009602:Nitrosamines; D009928:Organ Specificity; D010179:Pancreas / Q000737:chemistry / Q000166:cytology / Q000473:pathology; D010190:Pancreatic Neoplasms / Q000139:chemically induced / Q000276:immunology / Q000473:pathology*; D011134:Polysaccharides / Q000032:analysis* / Q000737:chemistry
D000017:ABO Blood-Group System; D002273:Carcinogens; D009602:Nitrosamines; D011134:Polysaccharides; C013622:nitrosobis(2-oxopropyl)amine