[The cooperative effect of hydrocortisone, adrenaline, and high density lipoproteins in regulating the activity of multiple forms of liver hexokinase].
High density lipoproteins (HDL) were shown to modulate the effect of hydrocortisone and adrenaline on activity of hexokinase in surviving liver tissue slices. The hormones inhibited the enzymatic activity while in presence of HDL the hormones exhibited an opposite effect. The phenomenon observed was termed a "cooperative effect"; it could not be reproduced by any single components of the system studied. Mechanism of the effect involved induction of all the multiple hexokinase forms which was abolished by actinomycin D. A specific function could be ascribed only to HDL3; HDL2, low and very low density lipoproteins did not exhibit any effects. The cooperative effect of HDL and of the adaptive hormones was also inhibited in the surviving liver tissue slices by vinblastine, gordox and methylamine thus demonstrating the sophisticated nature of the mechanism.
Panin
L E
LE
Russkikh
G S
GS
Atuchina
N V
NV
Tret'iakova
T A
TA
D000818:Animals; D004837:Epinephrine / Q000502:physiology*; D005260:Female; D006593:Hexokinase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D006854:Hydrocortisone / Q000502:physiology*; D066298:In Vitro Techniques; D007527:Isoenzymes / Q000037:antagonists & inhibitors / Q000378:metabolism*; D008075:Lipoproteins, HDL / Q000502:physiology*; D008099:Liver / Q000201:enzymology*; D051381:Rats; D017208:Rats, Wistar
D007527:Isoenzymes; D008075:Lipoproteins, HDL; D006593:Hexokinase; D006854:Hydrocortisone; D004837:Epinephrine
O kooperativnom éffekte gidrokortizona, adrenalina i lipoproteidov vysokoĭ plotnosti v reguliatsii aktivnosti mnozhestvennykh form geksokinazy v pecheni.