In situ quantitative estimates of the age-related and diabetes-related changes in cerebral endothelial barrier antigen.
To determine whether ageing or diabetes, conditions known to alter the blood-brain barrier (BBB) is associated with changes in endothelial barrier antigen (EBA), a rat BBB-specific protein, in situ quantitation of forty vibrotome sections of cerebral tissue of 5 young (4 months old), 5 aged (26 months old) and 5 streptozotocin-induced diabetic rats after 4 weeks of diabetes were studied using immunohistologic techniques. The anti-EBA-stained microvessels were normalized against the total microvessels identified with either anti-EBA binding or anti-Glut-1 binding. The results indicate that the EBA-stained microvessels of the hippocampus, but not of other cerebral cortical areas or white matter, are reduced in aged rats (88.02 +/- 2.19% vs. 80.65 +/- 2.44%, P < 0.01). There were no significant changes in the diabetic rats. These results support the notion that changes in the BBB correlate with the aging of the central nervous system.
Mooradian
A D
AD
St. Louis V.A. Medical Center, MO.
Grabau
G
G
Uko-eninn
A
A
D000375:Aging / Q000276:immunology / Q000502:physiology*; D000818:Animals; D000954:Antigens, Surface / Q000032:analysis*; D001812:Blood-Brain Barrier / Q000502:physiology*; D002540:Cerebral Cortex / Q000098:blood supply / Q000502:physiology; D002560:Cerebrovascular Circulation / Q000502:physiology; D003921:Diabetes Mellitus, Experimental / Q000276:immunology / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000276:immunology; D051272:Glucose Transporter Type 1; D006624:Hippocampus / Q000098:blood supply / Q000502:physiology; D007150:Immunohistochemistry; D008297:Male; D008833:Microcirculation / Q000502:physiology; D009004:Monosaccharide Transport Proteins / Q000276:immunology / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000032:analysis*; D051381:Rats; D011916:Rats, Inbred F344
D000954:Antigens, Surface; D051272:Glucose Transporter Type 1; D009004:Monosaccharide Transport Proteins; D009419:Nerve Tissue Proteins