[DNA extraction from hard dental tissues].
Two different standard ways of DNA extraction (salting out and phenol-chloroform methods) were assayed in order to recovery nucleic acids from dental tissues. The DNA extracted was tested for purity by means of transverse alternating field electrophoresis (TAFE) using Saccharomyces cerevisiae chromosomes as markers. Both extraction methods give similar qualitative and quantitative results being a DNA yield from hard dental tissues approximately 30% of those extracted from the whole tooth. Our results indicate salting out as a preferable method due to its rapidity and usefulness.
Laboratorio Centralizzato per la Ricerca Scientifica, Facoltà di Medicina e Chirurgia, Università degli Studi di Catania.
M
M
Avitabile
G
G
Dell'Osso
R
R
Rasá
F
F
Tripi
N E
NE
Campagna
G A
GA
Magrí
G
G
Sciacca
A
A
Rasá
D004247:DNA / Q000032:analysis / Q000302:isolation & purification*; D004586:Electrophoresis / Q000295:instrumentation / Q000379:methods; D006801:Humans; D013056:Spectrophotometry, Ultraviolet; D014070:Tooth / Q000737:chemistry*
D004247:DNA
Estrazione di DNA dai tessuti duri del dente.