Tumor imaging with technetium-99m-DTPA encapsulated in RES-avoiding liposomes.
For passive targeting of liposomes to tumor tissues, we earlier developed reticuloendothelial system (RES)-avoiding liposomes modified with a uronic acid derivative, palmityl-D-glucuronide (PGlcUA). In the present study, we encapsulated technetium-99m (99mTc)-diethylenetriaminepentaacetic acid (DTPA) in PGlcUA-liposomes (dipalmitoylphosphatidylcholine:cholesterol:PGlcUA = 40:40:20 as a molar ratio) and studied the biodistribution of the liposomes in tumor-bearing mice. 99mTc-DTPA encapsulated in liposomes effectively accumulated in tumor tissues after intravenous administration. Corresponding to these results, tumor was strongly imaged by a gamma-camera when 99mTc-DTPA-encapsulated PGlcUA-liposomes were used.
Department of Radiobiochemistry, School of Pharmaceutical Sciences, University of Shizuoka, Japan.
N
N
Oku
Y
Y
Namba
A
A
Takeda
S
S
Okada
D015060:1,2-Dipalmitoylphosphatidylcholine; D000818:Animals; D004337:Drug Carriers; D015902:Gamma Cameras; D007275:Injections, Intravenous; D008081:Liposomes; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D012157:Mononuclear Phagocyte System / Q000378:metabolism*; D010715:Phosphatidylglycerols; D011877:Radionuclide Imaging; D012510:Sarcoma 180 / Q000000981:diagnostic imaging* / Q000378:metabolism; D016284:Technetium Tc 99m Pentetate / Q000008:administration & dosage* / Q000493:pharmacokinetics; D014018:Tissue Distribution
D004337:Drug Carriers; D008081:Liposomes; D010715:Phosphatidylglycerols; D015060:1,2-Dipalmitoylphosphatidylcholine; C030345:1,2-dipalmitoylphosphatidylglycerol; D016284:Technetium Tc 99m Pentetate