Bacteriophage PRD1 proteins: cross-linking and scanning transmission electron microscopy analysis.
Bacteriophage PRD1, a double-stranded DNA virus infecting Escherichia coli, has a membrane inside the protein capsid. Chemical cross-linking and scanning transmission electron microscopy showed that the multimeric major coat protein (P3) exists in a trimeric form. Cross-linking revealed, in addition, that protein P11, located between the protein coat and the membrane, exists also as a homotrimer. Minor protein P7 was associated with the major coat protein P3. Under nonreducing conditions the infectivity proteins P16 and P18 formed homomultimeric complexes which were dissociated upon addition of 2-mercaptoethanol.
Department of Genetics, University of Helsinki, Finland.
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Luo
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Hantula
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Tichelaar
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Bamford
D002213:Capsid / Q000737:chemistry* / Q000648:ultrastructure; D003090:Coliphages / Q000737:chemistry* / Q000648:ultrastructure; D003432:Cross-Linking Reagents; D017348:Microscopy, Electron, Scanning Transmission; D008970:Molecular Weight; D011487:Protein Conformation; D013388:Succinimides
D003432:Cross-Linking Reagents; D013388:Succinimides; C011240:dithiobis(succinimidylpropionate)