Porphyromonas-like gram-negative rods in naturally occurring periodontitis in dogs.
A total of 259 Gram-negative Porphyromonas-like rods isolated from subgingival plaque samples of 16 family-owned dogs with naturally occurring periodontitis were characterized phenotypically by biochemical reactions, metabolic end products and enzymatic activities (API-ZYMTM, RoscoTM). Four distinct groups were found. Group A isolates (63) were asaccharolytic, lipase negative, trypsin positive and produced phenylacetic acid (PAA) from peptone-yeast extract glucose broth. Unlike P. gingivalis strains they were catalase positive. Group B isolates (42) differed from those of group A by a positive lipase reaction and from those of group D by failing to ferment sugars. Group C isolates (88) were asaccharolytic and did not produce PAA. They were alpha-fucosidase, N-acetyl-beta-glucosaminidase (beta-NAG) and trypsin negative, resembling P. endodontalis, but unlike human isolates, they were catalase positive. Subgroup C.1 isolates (6) differed from those of parent group C by producing minor amounts of PAA, and subgroup C.2 isolates (12) were beta-NAG positive. Group D isolates (46) were weakly fermentative, lipase, catalase and trypsin positive, and produced PAA. They resembled the B (P.) salivosus type strain which, in our hands, fermented weakly glucose, lactose and mannose. Two isolates could not be assigned to any of the previous groups.
Karjalainen
J
J
National Public Health Institute, Anaerobe Reference Laboratory, Helsinki, Finland.
Kanervo
A
A
Väisänen
M L
ML
Forsblom
B
B
Sarkiala
E
E
Jousimies-Somer
H
H
D000818:Animals; D001426:Bacterial Proteins / Q000032:analysis; D015373:Bacterial Typing Techniques; D001438:Bacteroidaceae / Q000145:classification / Q000302:isolation & purification* / Q000378:metabolism; D050260:Carbohydrate Metabolism; D002374:Catalase / Q000032:analysis; D003773:Dental Plaque / Q000382:microbiology; D004283:Dog Diseases / Q000382:microbiology*; D004285:Dogs / Q000382:microbiology; D010518:Periodontitis / Q000382:microbiology / Q000662:veterinary*; D010641:Phenotype; D010648:Phenylacetates / Q000378:metabolism; D013045:Species Specificity
D001426:Bacterial Proteins; D010648:Phenylacetates; D002374:Catalase; C025136:phenylacetic acid