Hyaluronic acid and purine nucleoside phosphorylase in vascular and luminal effluents of small bowel grafts as parameters of preservation injury.
Reliable parameters reflecting the degree of graft injury after small bowel preservation are currently not established. We investigated hyaluronic acid (HA) and purine nucleoside phosphorylase (PNP) as indicators of preservation injury before small bowel transplantation. In the first part of the study, intestinal grafts were harvested, perfused with saline, and flushed either immediately or after 1, 6, 12, 24, and 48 hr of cold storage (n = 6/group). HA and PNP were assayed in vascular and luminal effluents. In the second part of the study, 24 grafts were transplanted after preservation periods of 1, 6, 9, and 12 hr (n = 6/group) to assess if HA and PNP are predictors of postoperative graft survival. HA levels in vascular effluents and PNP activities in luminal effluents correlated with duration of preservation time and predicted graft survival. Utilizing both parameters significantly increased the predictive accuracy.
Department of Surgery, University of Pittsburgh, Pennsylvania 15261.
A R
AR
Mueller
P N
PN
Rao
J T
JT
Snyder
R A
RA
Hoffman
W H
WH
Schraut
D000818:Animals; D002463:Cell Membrane Permeability; D006820:Hyaluronic Acid / Q000032:analysis*; D007421:Intestine, Small / Q000737:chemistry / Q000637:transplantation*; D008297:Male; D011683:Purine-Nucleoside Phosphorylase / Q000032:analysis*; D051381:Rats; D011917:Rats, Inbred Lew; D014021:Tissue Preservation
D006820:Hyaluronic Acid; D011683:Purine-Nucleoside Phosphorylase