Fc gamma R-expressing monocytes are necessary for PWM-induced proliferation of Saimiri sciureus peripheral blood mononuclear cells.
When used as a polyclonal activator of blood immunocompetent cells in the squirrel monkey, pokeweed mitogen (PWM) failed to induce lymphoproliferation unless either autologous or human serum was added to the culture medium, instead of a serum assumed to be deprived of Ig such as foetal bovine serum. We thus attempted to delineate activation processes initiated by PWM and which required both monocytes and native Ig molecules. For instance, the physical presence of live monocytes within lymphocyte suspensions vs. paraformaldehyde-fixed monocytes or products of monocytic origin was an absolute prerequisite for inducing squirrel monkey lymphocytes to proliferate in the presence of PWM. In addition, a combination of monocytes and native Ig were further shown to induce lymphocytes to proliferate in the presence of PWM. We therefore questioned whether Fc gamma R on the surface of monocytes vs. Fc gamma R expressed by other cells were involved in the initiation of PWM-driven lymphoproliferation. PWM may represent a useful tool for studies of Fc gamma R-mediated immunological processes.
Laboratoire d'Immunologie Parasitaire, Institut Pasteur de Guyane, Cayenne, French Guiana.
O
O
Garraud
D
D
Blanchard
R
R
Perraut
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D005260:Female; D007074:Immunoglobulin G / Q000096:biosynthesis; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology; D008297:Male; D009000:Monocytes / Q000276:immunology*; D011043:Pokeweed Mitogens; D017452:Receptors, IgG / Q000276:immunology*; D012453:Saimiri
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D011043:Pokeweed Mitogens; D017452:Receptors, IgG