Production of human antibodies using bacteriophage.
The immune system produces antibodies by a process of antigen-driven selection. An in vitro process of antigen-driven selection, based on the display of antibody fragments on filamentous bacteriophage, has recently been developed. This enables human antibody fragments of high affinity and specificity to be produced without immunization.
Griffiths
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MRC Centre for Protein Engineering, MRC Centre, Cambridge, UK.
D000818:Animals; D000915:Antibody Affinity; D000917:Antibody Formation; D000918:Antibody Specificity; D001402:B-Lymphocytes / Q000276:immunology; D001435:Bacteriophages / Q000235:genetics*; D002213:Capsid / Q000235:genetics; D015723:Gene Library; D015321:Gene Rearrangement; D005803:Genes, Immunoglobulin; D005813:Genes, Synthetic; D005814:Genes, Viral; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D007135:Immunoglobulin Variable Region / Q000235:genetics; D008213:Lymphocyte Activation; D051379:Mice; D015202:Protein Engineering / Q000379:methods*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D015678:Viral Structural Proteins / Q000235:genetics
D007140:Immunoglobulin Fab Fragments; D007135:Immunoglobulin Variable Region; D011993:Recombinant Fusion Proteins; D015678:Viral Structural Proteins