Occurrence and sequence of a DnaJ protein in plant (Allium porrum) epidermal cells.
Antibodies raised against a purified fraction from microsomal membranes of leek epidermal cells were used to screen a lambda zap expression library from epidermal cells of leak plants. A near full-length clone was isolated. This cDNA contains an open reading-frame of 1,191 bp coding for a DnaJ protein (leek DNAJ 1 or LDJ1). Leek DnaJ1 represents the second protein of this type described in a pluricellular organism, the first being that sequenced from human cells.
Plant Research Laboratory, Michigan State University.
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Bessoule
D000490:Allium / Q000737:chemistry* / Q000235:genetics; D000595:Amino Acid Sequence; D001483:Base Sequence; D004247:DNA; D050956:HSP40 Heat-Shock Proteins; D006360:Heat-Shock Proteins / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000032:analysis* / Q000235:genetics; D017386:Sequence Homology, Amino Acid
C081312:DnaJ protein, Allium porrum; D050956:HSP40 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D010940:Plant Proteins; D004247:DNA