Protein-synthesis inhibition induces perichromatin granule accumulation and intranuclear rodlet formation in osmotically stimulated supraoptic neurons.
The distribution of perichromatin granules (PGs), a storage form of pre-mRNAs, was studied in supraoptic neurons of control and osmotically stimulated rats, and also after treatment with cycloheximide, a protein-synthesis inhibitor. In non-cycloheximide-treated rats, neuronal activation by dehydration significantly decreased the number of PGs. Conversely, PGs were drastically increased in the supraoptic neurons of dehydrated rats treated with cycloheximide for 4 h. This suggests that cycloheximide does not interfere with the transcriptional activation induced by dehydration, but it affects the processing of newly synthesized pre-mRNAs. Moreover, protein-synthesis inhibition was associated with the formation of intranuclear bundles of tubular filaments.
Department of Anatomy and Cell Biology, Faculty of Medicine, Santander, Spain.
M
M
Lafarga
M T
MT
Berciano
M A
MA
Andres
D000818:Animals; D002467:Cell Nucleus / Q000187:drug effects / Q000648:ultrastructure*; D002843:Chromatin / Q000187:drug effects / Q000648:ultrastructure*; D003513:Cycloheximide / Q000494:pharmacology; D008297:Male; D008854:Microscopy, Electron; D009474:Neurons / Q000187:drug effects / Q000648:ultrastructure*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012965:Sodium Chloride / Q000494:pharmacology; D013495:Supraoptic Nucleus / Q000166:cytology / Q000187:drug effects / Q000648:ultrastructure*
D002843:Chromatin; D012965:Sodium Chloride; D003513:Cycloheximide