In vivo distribution of radio-labeled tumor infiltrating lymphocytes in cancer patients.
Tumor-infiltrating lymphocytes have proven effective in murine tumor models and preliminary clinical trials. To evaluate the efficacy of these lymphocytes in treatment attempts were made to study the in vivo migration and distribution of these lymphocytes. Tumor-infiltrating lymphocytes isolated from five patients with malignant metastatic breast cancer or melanoma cultured and expanded in vitro with low-dose of recombinant interleukin-2 were labeled with 111Indium-oxine and infused to the patients. A large view gamma camera was used to evaluate the distribution and localization of the infused TIL. Localization of 111In-labeled TIL in the lungs was seen within two hours after infusion and high levels of radioactivity were observed at 24 hours in lungs, liver and spleen. The activity in the lungs diminished after 72 hours. No specific localization of 111In-labeled TIL was observed in the metastatic sites.
Department of Cancer Research, Dr. L. Willems Instituut, Diepenbeek, Belgium.
Y
Y
Chin
J
J
Janssens
J
J
Bleus
J
J
Zhang
J
J
Raus
D000293:Adolescent; D000328:Adult; D001943:Breast Neoplasms / Q000000981:diagnostic imaging / Q000276:immunology*; D002470:Cell Survival / Q000502:physiology; D005260:Female; D006801:Humans; D016130:Immunophenotyping; D007205:Indium Radioisotopes; D016246:Lymphocytes, Tumor-Infiltrating / Q000276:immunology*; D008297:Male; D008545:Melanoma / Q000000981:diagnostic imaging / Q000276:immunology*; D008875:Middle Aged; D011877:Radionuclide Imaging; D014407:Tumor Cells, Cultured
D007205:Indium Radioisotopes