[Generation of a monoclonal antibody specific to human T cell receptor V beta 22, and analysis of peripheral blood T cell repertoire utilizing this monoclonal antibody].
A murine monoclonal antibody, R1-21, against a HLA-DR4 restricted CD4+ T cell line specific to streptococcal cell wall (SCW) Ag was generated. R1-21 reacted with about 50% of the HLA-DR4 restricted CD4+ T cell line. R1-21 distinguished a diversity of TCR because it reacted with a small population of peripheral T lymphocytes, induced proliferative response in peripheral blood lymphocytes, immunoprecipitated a cell surface molecule of 80 kDa in non-reduced condition and 45 and 40 kDa in reduced condition, and the immunoprecipitates with R1-21 was precleared with a known anti-TCR mAb. Sequence analysis of cDNA clones corresponding to TCR alpha and beta genes derived from R1-21 positive and negative T cell lines revealed that R1-21 recognized a V beta 22 gene product. By utilizing R1-21, it was found that V beta 22 positive cells existed 3.80 +/- 0.94% and 3.56 +/- 0.59% in healthy adult and neonatal cord blood T lymphocytes, respectively, and a V beta 22 gene product was skewed expressed on CD4+ T cells. (CD 4/CD 8 ratio: 1.69 +/- 0.57).
Department of genetics, Kyushu University 69, Fukuoka.
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Okamoto
D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D015704:CD4 Antigens; D016516:CD4-CD8 Ratio; D002478:Cells, Cultured; D015804:HLA-DR4 Antigen; D006801:Humans; D007231:Infant, Newborn; D007958:Leukocyte Count; D051379:Mice; D016133:Polymerase Chain Reaction; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology
D000911:Antibodies, Monoclonal; D015704:CD4 Antigens; D015804:HLA-DR4 Antigen; D016693:Receptors, Antigen, T-Cell, alpha-beta