Isolation and influenza virus receptor activity of glycophorins B, C and D from human erythrocyte membranes.
(1) Glycophorins (GPs) AM, AN, B, C and D were each isolated into a high state of purity from human erythrocyte membranes by a combination of lithium diiodosalicylate (LIS)-phenol extraction, gel-filtration with Bio-Gel A1.5m and HPLC with LiChrospher 1000 TMAE. (2) GPs-B, -C and -D reacted with influenza A and B viruses as well as GPs-AM and -AN and the order of reactivities against two viruses of the glycophorins was as follows: GP-B > GP-C > GP-AM = GP-AN >> GP-D for the former virus and GP-C > GP-B > GP-AM = GP-AN >> GP-D for the latter virus.
Department of Biochemistry, Tokyo College of Pharmacy, Japan.
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K
Ohyama
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T
Endo
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S
Ohkuma
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T
Yamakawa
D000596:Amino Acids / Q000032:analysis; D002241:Carbohydrates / Q000032:analysis; D004910:Erythrocyte Membrane / Q000378:metabolism*; D006021:Glycophorins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D006384:Hemagglutination / Q000187:drug effects; D006801:Humans; D009975:Orthomyxoviridae / Q000378:metabolism*
D000596:Amino Acids; D002241:Carbohydrates; D006021:Glycophorins