Reduced shear stress: a major component in the ability of mammalian tissues to form three-dimensional assemblies in simulated microgravity.
BHK-21 cells were cultured under various shear stress conditions in an Integrated Rotating-Wall Vessel (IRWV). Shear ranged from 0.5 dyn/cm2 (simulated microgravity) to 0.92 dyn/cm2. Under simulated microgravity conditions, BHK-21 cells complexed into three-dimensional cellular aggregates attaining 6 x 10(6) cells/ml as compared to growth under 0.92 dyn/cm2 conditions. Glucose utilization in simulated microgravity was reduced significantly, and cellular damage at the microcarrier surface was kept to a minimum. Thus, the integrated rotating wall vessel provides a quiescent environment for the culture of mammalian cells.
Goodwin
T J
TJ
KRUG Life Sciences, Houston, Texas 77058.
Prewett
T L
TL
Wolf
D A
DA
Spaulding
G F
GF
D000818:Animals; D002449:Cell Aggregation / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D002460:Cell Line; D006224:Cricetinae; D006112:Gravitation; D007668:Kidney / Q000166:cytology / Q000201:enzymology / Q000378:metabolism*; D008855:Microscopy, Electron, Scanning; D012399:Rotation; D013314:Stress, Mechanical