The benefit of a single-passage perfusion with a hypertonic citrate solution on the cold ischemia tolerance of epigastric free flaps in Dark-Agouty rats.
In this study the effectiveness of a single-passage perfusion with hypertonic citrate solution on the cold ischemia tolerance in epigastric free flaps in Dark-Agouty rats was investigated. In the control group a significant decline in survival percentage was observed when the storage time was extended from 72 hours to 96 hours. A single (pressure controlled) passage perfusion with hypertonic citrate solution was able to prevent this decline.
Department of Plastic, Reconstructive, and Hand Surgery, University Hospital, Utrecht, The Netherlands.
P M
PM
Werker
C J
CJ
Green
M
M
Kon
M L
ML
Overgoor
R J
RJ
Franken
D000818:Animals; D002951:Citrates / Q000494:pharmacology*; D019343:Citric Acid; D015925:Cryopreservation; D006085:Graft Survival / Q000187:drug effects*; D006982:Hypertonic Solutions; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013524:Surgical Flaps / Q000502:physiology*; D014021:Tissue Preservation
D002951:Citrates; D006982:Hypertonic Solutions; D019343:Citric Acid