The potential value of integral membrane proteins in the vaccination of lambs against Haemonchus contortus.
An extract of adult Haemonchus contortus enriched in the parasite's intestinal microvillar membrane protein H11 and other integral membrane proteins but free of the protein contortin was evaluated as a potential vaccine in two breeds of sheep. The worm burdens of Clun Forest sheep injected with the extract and challenged with 25,000 infective larvae were reduced 89% by weight compared to the average for the controls. The worm burdens of Dorset sheep (challenged with 10,000 infective larvae) were reduced 72%. In both breeds the reduction in the number of female worms, 92 and 71.8%, respectively, was greater than the reduction in the males (86.5 and 46%). Parasite egg output, determined only for the Dorsets, was reduced 92% protection correlated with serum antibody titre. Most of the antibodies were directed against H11.
Immunology Department, AFRC Institute of Animal Physiology and Genetics Research, Babraham, Cambridge, U.K.
E A
EA
Munn
T S
TS
Smith
M
M
Graham
A S
AS
Tavernor
C A
CA
Greenwood
D000818:Animals; D005260:Female; D006188:Haemonchiasis / Q000517:prevention & control / Q000662:veterinary*; D006189:Haemonchus / Q000276:immunology*; D015801:Helminth Proteins / Q000276:immunology; D008297:Male; D008565:Membrane Proteins / Q000276:immunology*; D012756:Sheep; D012757:Sheep Diseases / Q000517:prevention & control*; D014611:Vaccination / Q000662:veterinary*
D015801:Helminth Proteins; D008565:Membrane Proteins