Application of in vivo bioluminescence to the study of ionophoretic action.
Ionophores (carbonyl cyanide m-chlorophenylhydrazone; valinomycin; and the hop-derived compounds colupulone, trans-isohumulone and trans-humulinic acid) reduced the rate of dodecanal-dependent light emission from IuxA/B-transformed cells of Lactococcus lactis subsp. diacetylactis F712 when the cells were suspended in a buffered medium (pH 6.4) containing glucose. This allowed an assay for ionophores to be devised and permitted measurement of the effects of such compounds on the test organism by the use of a non-destructive technique in real time.
Simpson
W J
WJ
BRF International, Nutfield, Surrey, UK.
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000494:pharmacology; D003512:Cyclohexanones / Q000494:pharmacology; D003517:Cyclopentanes / Q000494:pharmacology; D005798:Genes, Bacterial; D006863:Hydrogen-Ion Concentration; D007476:Ionophores / Q000032:analysis* / Q000494:pharmacology*; D013294:Lactococcus lactis / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D008027:Light; D008156:Luciferases / Q000235:genetics / Q000378:metabolism*; D008163:Luminescent Measurements; D014169:Transformation, Bacterial; D014634:Valinomycin / Q000494:pharmacology; D014733:Vibrio / Q000201:enzymology / Q000235:genetics
D000900:Anti-Bacterial Agents; D003512:Cyclohexanones; D003517:Cyclopentanes; D007476:Ionophores; D014634:Valinomycin; C075356:colupulone; C081210:humulinic acid; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone; C081209:isohumulone; D008156:Luciferases