Substitution of lysine for arginine in the N-terminal 217th amino acid residue of the H gamma II of Staphylococcal gamma-hemolysin lowers the activity of the toxin.
The staphylococcal toxin gamma-hemolysin consists of two protein components, LukF and H gamma II. Staphylococcus aureus P83 was found to have five components, LukF, LukF-PV, LukM, LukS, and H gamma II for leukocidin or gamma-hemolysin. H gamma II of S. aureus P83 was demonstrated to be a naturally-occurring analogous molecule of H gamma II [H gamma II(P83)], in which the 217th arginine residue was replaced by lysine. The H gamma II(P83) showed about 50% of the hemolytic activity of normal H gamma II in the presence of LukF.
Department of Applied Biological Chemistry, Faculty of Agriculture, Tohoku University, Sendai, Japan.
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Sudo
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Choorit
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Asami
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Kaneko
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Muramoto
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Kamio
D000595:Amino Acid Sequence; D001120:Arginine / Q000737:chemistry*; D001426:Bacterial Proteins; D001427:Bacterial Toxins / Q000737:chemistry* / Q000302:isolation & purification / Q000633:toxicity; D001483:Base Sequence; D002845:Chromatography / Q000379:methods; D004912:Erythrocytes / Q000187:drug effects; D006460:Hemolysin Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000633:toxicity; D006461:Hemolysis; D006801:Humans; D007956:Leukocidins / Q000737:chemistry / Q000302:isolation & purification / Q000633:toxicity; D008239:Lysine / Q000737:chemistry*; D008969:Molecular Sequence Data; D015202:Protein Engineering; D013329:Structure-Activity Relationship
D001426:Bacterial Proteins; D001427:Bacterial Toxins; D006460:Hemolysin Proteins; D007956:Leukocidins; C037956:gamma-hemolysin, Staphylococcus aureus; D001120:Arginine; D008239:Lysine