Study of the susceptibility of human colorectal tumor explants to LAK-cell lysis: comparison of various cytotoxicity tests.
The susceptibility of tumor cells from cultures of 8 human colorectal tumor explants to a standard LAK preparation originating from a single healthy donor was assessed in 5 various cytotoxic tests. Target cells were suspended or attached (in three different 51Cr-release 4 hr tests), grown as a confluent monolayer (in a colorimetric 72 hr test) and grown as three-dimensional spheroids (in a 51Cr-release 24 hr assay). The susceptibility to lysis observed for one tumor in the 5 tests varied from 4 to 15 times and was a tumor attribute independent of the susceptibility to lymphocyte attack. There was no correlation of results between the 4 hr tests and a 72 hr test performed on monolayer, or the spheroid test taking into account the resistance of the three-dimensional structure to lymphocyte invasion. Our study excludes the possibility of quantitative interpolation of assessments of tumor cell susceptibility to lymphocytes received from various tests. It also refers to simple reductionist tests which may be compared, however, after ranking the qualitative categories.
Center of Oncology, Maria Sklodowska-Curie Memorial Institute, Krakow, Poland.
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Ochalek
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von Kleist
D015179:Colorectal Neoplasms / Q000276:immunology*; D003601:Cytotoxicity Tests, Immunologic / Q000379:methods*; D003602:Cytotoxicity, Immunologic; D005069:Evaluation Studies as Topic; D006801:Humans; D066298:In Vitro Techniques; D015979:Killer Cells, Lymphokine-Activated / Q000276:immunology*; D014407:Tumor Cells, Cultured