Identification of 2-amino-2,6-dideoxy-D-glucose (D-quinovosamine), isolated from the cell walls of the alkaliphilic Bacillus sp. Y-25, by 500-MHz 1H NMR spectroscopy.
Cell walls of alkaliphilic Bacillus strain Y-25 are composed of gamma-peptidoglycan and two acidic polymers. An amino sugar, which was a min component of one acidic polymer, did not correspond to any of the commercially available hexosamines. The amino sugar was isolated from the hydrolysate of the acidic polymer, purified, and identified as D-quinovosamine (2-amino-2,6- dideoxy-D-glucose) by 500-MHz NMR spectroscopic analysis and polarimetry.
Department of Bioengineering, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Japan.
M
M
Ito
R
R
Aono
K
K
Horikoshi
D001407:Bacillus / Q000737:chemistry*; D002241:Carbohydrates / Q000032:analysis; D002473:Cell Wall / Q000737:chemistry; D002848:Chromatography, DEAE-Cellulose; D002852:Chromatography, Ion Exchange; D005944:Glucosamine / Q000031:analogs & derivatives* / Q000032:analysis / Q000302:isolation & purification; D006859:Hydrogen; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D010457:Peptidoglycan / Q000737:chemistry* / Q000302:isolation & purification
D002241:Carbohydrates; D010457:Peptidoglycan; D006859:Hydrogen; D005944:Glucosamine; C009149:6-desoxy-D-glucosamine