Expression of constitutive heat shock protein-70 in normal (non-stressed) rabbit urinary bladder tissue.
The expression of constitutive HSP-70 in the urinary bladder was determined by SDS-PAGE and western blotting using a mouse monoclonal antibody against HSP-70. The western blot analysis showed that the mouse anti-HSP-70 cross-reacted with a 70 kDa protein present in the extracts of the urinary bladder muscle and mucosa. Densitometric scanning of the western blots allowed us to specifically quantitate the relative amounts of the HSP-70. The quantitation of the HSP-70 by combining immunoblotting and densitometry using a laser scanner is reproducible and this technique requires only a small amount of tissue. The amounts of HSP-70 can be estimated from a standard curve of nanogram(ng) of HSP-70 vs absorption from the immunoblots. The amounts of HSP-70 in the muscular and mucosal layers in the body of the urinary bladder are more than those in the base of the bladder. The presence of HSP-70 in the muscle and mucosal epithelium of the bladder was demonstrated by immunohistochemical analysis of freshly removed tissue from the base and the body of bladder from normal animals.
Division of Urology, University of Pennsylvania 19104.
Y
Y
Zhao
R M
RM
Levin
F C
FC
Monson
S
S
Chacko
D000818:Animals; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D004848:Epithelium / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000276:immunology / Q000378:metabolism*; D066298:In Vitro Techniques; D009132:Muscles / Q000378:metabolism; D011817:Rabbits; D001743:Urinary Bladder / Q000378:metabolism*
D006360:Heat-Shock Proteins