In vitro and in vivo evaluations of Chinese traditional (kampo) medicines as anticalculus agents in the rat.
Inhibitory effects of two Chinese traditional (Kampo) medicines, Shigyaku-san and Shikunshi-to, on the in vitro formation of calcium phosphate precipitates and the in vivo deposition of supragingival dental calculus were studied. Both had inhibitory effects on hydroxyapatite transformation and induction; effects of Shigyaku-san and Shikunshi-to were 1/100 and 1/400 of that of 1-hydroxyethylidene-1,1-bisphosphonate (HEBP). When used topically (in drinking water) for 4 weeks, 20-40 mg/ml of Shigyaku-san or Shikunshi-to reduced the formation of dental calculus by 40-60%, while 0.03% (w/v) HEBP decreased it by 70-98%. However, when these drugs were given by gastric intubation for 2 weeks, they were all ineffective, suggesting that the observed anticalculus effects were of topical rather than of systemic origin.
Hidaka
S
S
Department of Oral Biochemistry, Fukuoka Dental College, Japan.
Abe
K
K
Liu
S Y
SY
D000287:Administration, Topical; D000704:Analysis of Variance; D000818:Animals; D002130:Calcium Phosphates / Q000378:metabolism; D002614:Chelating Agents; D003728:Dental Calculus / Q000517:prevention & control*; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000627:therapeutic use*; D004533:Egtazic Acid / Q000627:therapeutic use; D012968:Etidronic Acid / Q000627:therapeutic use; D006882:Hydroxyapatites / Q000378:metabolism; D008297:Male; D051381:Rats; D017208:Rats, Wistar
D002130:Calcium Phosphates; D002614:Chelating Agents; D004365:Drugs, Chinese Herbal; D006882:Hydroxyapatites; D004533:Egtazic Acid; D012968:Etidronic Acid