Solution conformation of the tetrasaccharide glycoside Xyl beta 1-(Man alpha 1-3)2Man beta 1-4Glc beta 1R from the mollu-series glycosphingolipids.
The conformation of Xyl beta 1-(Man alpha 1-3)2Man beta 1-4Glc beta 1-OCH2CH(N3)CH(OH) CH = CHC13H27 tetrasaccharide glycoside in Me2SO-d6 was investigated with use of a distance mapping procedure based on rotating-frame NOE and hydrogen bond constraints, and Molecular Mechanics calculations. The Man alpha 1-3Man beta linkage was found to be well defined in a single conformation, whereas some flexibility could be assumed for the remaining two glycosidic linkages. Similar conclusions, though based on a smaller number of constraints, were drawn for the title compound anchored in mixed D2O/dodecylphosphocholine-d38 micelles.
Max-Planck-Institut für Medizinische Forschung, Heidelberg, Germany.
U
U
Dabrowski
J
J
Dabrowski
H
H
Grosskurth
C W
CW
von der Lieth
T
T
Ogawa
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D006028:Glycosphingolipids / Q000737:chemistry*; D006860:Hydrogen Bonding; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009844:Oligosaccharides / Q000737:chemistry* / Q000302:isolation & purification; D012996:Solutions
D006028:Glycosphingolipids; D009844:Oligosaccharides; D012996:Solutions