Intracellular calcium mobilization by inositol 1,4,5-trisphosphate: intracellular movements and compartmentalization.
Intracellular calcium ion (Ca2+) changes in NIH-3T3 fibroblasts responding to inositol 1,4,5-trisphosphate (IP3) injections have been monitored using high resolution digital imaging of the calcium indicator Fura-2. Ester loaded and microinjected indicator report radically different patterns of Ca2+ change during the IP3 response. These differences arise from intracellular compartmentalization of the ester loaded indicator which can seriously distort reported Ca2+ levels. Prominent among these aberrant responses is a signal in which Ca2+ levels in the cell nucleus appear to exceed those in the rest of the cell, and an apparent slowing of the Ca2+ recovery time-course throughout the cell when temperature is increased. Similar behavior is observed in other cell types. Judicious use of both loading techniques can provide information on Ca2+ movements into organelles that might otherwise escape detection. The Ca2+ rise normally measured in bulk or integrated single cell measurements is a complex mix of cytosol/nucleus and organellar changes. Much, if not all, of the observable organellar change is an accumulation, not release, of Ca2+ following the IP3 injection. The Golgi apparatus is a conspicuous early site for this accumulation, and mitochondria show a large, temperature sensitive uptake that is capable of limiting the maximal Ca2+ change during the response.
Department of Neuroscience, Roche Institute of Molecular Biology, Nutley, New Jersey.
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Connor
D016475:3T3 Cells / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002451:Cell Compartmentation / Q000187:drug effects*; D016257:Fura-2; D006056:Golgi Apparatus / Q000378:metabolism; D006358:Hot Temperature; D015544:Inositol 1,4,5-Trisphosphate / Q000494:pharmacology*; D051379:Mice; D008928:Mitochondria / Q000378:metabolism; D008954:Models, Biological; D012503:Saponins / Q000494:pharmacology; D013696:Temperature
D012503:Saponins; D015544:Inositol 1,4,5-Trisphosphate; D002118:Calcium; D016257:Fura-2