[The kinetic and functional characteristics of DNA-dependent DNA-polymerases in Acholeplasma laidlawii PG-8].
The kinetic and functional characteristics of I and II forms of DNA-dependent DNA-polymerases of Acholeplasma laidlawii PG-8 have been studied. It is stated that I form of DNA polymerase possesses 5'-3'-exonuclease activity and is a typical replicase; II form of DNA-polymerase possesses both 5'-3'-polymerase and 3'-5'-exonuclease activity and is, evidently, a reparase. Both forms of enzyme give preference to poly(U)- and poly(A)-matrices having extremely high activity on these polymers. The enzymatic reactions realized by both forms of DNA-polymerases are described by the first-order equation. The calculated Michaelis-Menten constants equaled 180 and 250 microM for I and II forms of polymerases, respectively. It indicates that affinity to substrate in II form of polymerase is one-third higher than in I form of enzyme.
S V
SV
Bezuglyĭ
I G
IG
Skripal'
V V
VV
Babichev
D000128:Acholeplasma laidlawii / Q000201:enzymology*; D000818:Animals; D010582:Bacteriophage lambda; D002417:Cattle; D004247:DNA / Q000378:metabolism; D004269:DNA, Bacterial / Q000378:metabolism; D004279:DNA, Viral / Q000378:metabolism; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D013379:Substrate Specificity; D013950:Thymus Gland
D004269:DNA, Bacterial; D004279:DNA, Viral; D004247:DNA; D004259:DNA-Directed DNA Polymerase
Izuchenie kineticheskikh i funktsional'nykh kharakteristik DNK-zavisimykh DNK-polimeraz Acholeplasma laidlawii PG-8.