Method for sequential staining of GTL-banded metaphases with fluorescent-labeled chromosome-specific paint probes.
We describe a method for use of fluorescent-labeled whole chromosome-specific paint probes on GTL-banded metaphases to utilize the combined potential of these techniques for defining chromosome abnormalities. The efficacy of this method was tested on 6 cases involving different chromosome abnormalities and various tissues, including blood, amniotic fluid, skin fibroblasts, and bone marrow.
Section of Laboratory Genetics, Mayo Clinic, Rochester, Minnesota 55905.
S M
SM
Jalal
M E
ME
Law
E R
ER
Christensen
J L
JL
Spurbeck
G W
GW
Dewald
D000015:Abnormalities, Multiple / Q000235:genetics; D000022:Abortion, Spontaneous / Q000235:genetics; D000328:Adult; D000649:Amniocentesis; D002871:Chromosome Banding / Q000379:methods*; D005260:Female; D005456:Fluorescent Dyes; D005600:Fragile X Syndrome / Q000235:genetics; D006801:Humans; D006849:Hydrocephalus / Q000235:genetics; D017404:In Situ Hybridization, Fluorescence; D007223:Infant; D007231:Infant, Newborn; D007621:Karyotyping; D015473:Leukemia, Promyelocytic, Acute / Q000235:genetics; D008297:Male; D008677:Metaphase; D008875:Middle Aged; D009030:Mosaicism; D011247:Pregnancy; D014178:Translocation, Genetic
D005456:Fluorescent Dyes