The Vin-1 gene, identified by provirus insertional mutagenesis, is the cyclin D2.
The Vin-1 gene was initially identified as a gene whose expression is altered by the integration of proviruses in the Vin-1 common site of integration in retrovirus-induced rodent T-cell leukemias. We have now isolated the Vin-1 cDNA. Sequencing of the Vin-1 cDNA and Vin-1 exons revealed that the proviruses are integrated at the 5' end of the Vin-1 gene in an inverse transcriptional orientation. The sequence of the Vin-1 gene is identical to that of the recently identified G1-phase cyclin D2 gene. The human homolog of the Vin-1/cyclin D2 gene (CCND2) was mapped to chromosome 12, band p13.3, by in situ hybridization, confirming previous mapping data. Our results strongly support a role of the cyclin D2 gene in oncogenesis and thereby implicate altered cell cycle regulation in transformation.
Laboratory of Molecular Biology, Clinical Research Institute of Montreal, Quebec, Canada.
Z
Z
Hanna
M
M
Jankowski
P
P
Tremblay
X
X
Jiang
A
A
Milatovich
U
U
Francke
P
P
Jolicoeur
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000378:metabolism; D002471:Cell Transformation, Neoplastic; D002871:Chromosome Banding; D002874:Chromosome Mapping; D002881:Chromosomes, Human, Pair 12; D003001:Cloning, Molecular; D056742:Cyclin D2; D016213:Cyclins / Q000235:genetics*; D004273:DNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification; D005091:Exons; D015723:Gene Library; D006801:Humans; D015458:Leukemia, T-Cell / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D005810:Multigene Family; D016254:Mutagenesis, Insertional; D009857:Oncogenes; D011533:Proviruses / Q000235:genetics*; D051381:Rats; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D013154:Spleen / Q000378:metabolism; D014158:Transcription, Genetic; D016662:Virus Integration
C080336:CCND2 protein, human; C486196:Ccnd2 protein, mouse; C486197:Ccnd2 protein, rat; D056742:Cyclin D2; D016213:Cyclins; D004273:DNA, Neoplasm