Segment substitutions in alpha satellite DNA. Unusual structure of human chromosome 3-specific alpha satellite repeat unit.
We have sequenced the full-length copy of the alpha satellite higher-order repeated unit characteristic of human chromosome 3. Its internal structure, the regular alterations of J1 and J2 type monomers, is typical of the alphoid suprachromosomal family 1. This dimeric order is disrupted by the substitution of one J1 unit by an alien dimer which is not clearly related to any of the established monomeric types. We have also observed some other similar cases of segment substitutions in alpha satellite DNA. They probably represent a special type of molecular event which could be generated by gene conversion. Segment substitutions may be one of the important factors responsible for the extreme variability of localization patterns and actual sequences of alpha satellite DNA that should be taken into account in reconstructions of alpha satellite evolution.
Alexandrov
I A
IA
Laboratory of Cytogenetics, National Research Center of Mental Health, Moscow, Russia.
Mashkova
T D
TD
Romanova
L Y
LY
Yurov
Y B
YB
Kisselev
L L
LL
D005075:Biological Evolution; D002893:Chromosomes, Human, Pair 3; D004276:DNA, Satellite / Q000235:genetics*; D005785:Gene Conversion; D006801:Humans; D011995:Recombination, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
D004276:DNA, Satellite