Comparative effect of embryonic mouse fibroblasts (Balb/c-3T3) on the proliferation of hormone-dependent (T-47D) and hormone-independent (MDA-MB-231) human breast cancer cell lines.
The effects of cellular extracts (CE) and conditioned medium (CM) of embryonic mouse BALB/c-3T3 (clone A 31) cells on the proliferation and DNA content of hormone-dependent T-47D and hormone-independent MDA-MB-231 breast cancer cell lines were explored. After 6 days of culture, CE and CM provoke an intense proliferative effect in T-47D cells which correlates with DNA content. In contrast, in the MDA-MB-231 cells a significant inhibitory effect was observed. For both CE and CM the action was dose-dependent. In the T-47D cells, the CM can abolish the inhibitory effect provoked by the potent antiestrogen ICI 164,384. It is concluded that mouse embryonic BALB/c-3T3 cells contain factors which can stimulate or inhibit the growth of human mammary cancer cells.
C.N.R.S. Steroid Hormone Research Unit, Foundation for Hormone Research, Paris, France.
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G
Chetrite
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L
Delalonde
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JR
Pasqualini
D000818:Animals; D001943:Breast Neoplasms / Q000188:drug therapy*; D002455:Cell Division / Q000187:drug effects; D002457:Cell Extracts / Q000494:pharmacology*; D017077:Culture Media, Conditioned / Q000494:pharmacology; D004273:DNA, Neoplasm / Q000032:analysis*; D004336:Drug Antagonism; D004958:Estradiol / Q000031:analogs & derivatives / Q000494:pharmacology; D004965:Estrogen Antagonists / Q000494:pharmacology; D005347:Fibroblasts / Q000502:physiology; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C / Q000196:embryology; D009376:Neoplasms, Hormone-Dependent / Q000188:drug therapy*; D053284:Polyunsaturated Alkamides; D011980:Receptors, Progesterone / Q000187:drug effects*; D014407:Tumor Cells, Cultured
D002457:Cell Extracts; D017077:Culture Media, Conditioned; D004273:DNA, Neoplasm; D004965:Estrogen Antagonists; D053284:Polyunsaturated Alkamides; D011980:Receptors, Progesterone; D004958:Estradiol; C051781:ICI 164384