Mobilization of the penicillinase gene in Enterococcus faecalis.
Enterococcus faecalis SF4855 is a beta-lactamase-producing isolate resistant to high levels of gentamicin, with determinants for these resistances on the chromosome. SF4855 transferred both determinants into E. faecalis FA2-2 and UV202 at a frequency of 10(-9) in the presence of the MLS plasmid pYN120. beta-Lactamase and gentamicin resistance probes hybridized to three locations on the chromosome of FA2-2 transconjugants on contour-clamped homogeneous electric field electrophoresis. The study results suggest mobilization of the beta-lactamase determinant.
Department of Medicine, Wayne State University School of Medicine, Detroit, Michigan 48201.
J W
JW
Chow
M B
MB
Perri
L A
LA
Thal
M J
MJ
Zervos
D002876:Chromosomes, Bacterial; D004269:DNA, Bacterial / Q000032:analysis; D013293:Enterococcus faecalis / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D005798:Genes, Bacterial; D005839:Gentamicins / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D010405:Penicillinase / Q000235:genetics*; D010957:Plasmids
D004269:DNA, Bacterial; D005839:Gentamicins; D010405:Penicillinase