Liquid chromatographic determination of beta-cyclodextrin derivatives based on fluorescence enhancement after inclusion complexation.
A liquid chromatographic method using fluorescence detection for the determination of beta-cyclodextrin (beta CD) and its derivatives is presented. The chromatographic system is based on size-exclusion chromatography with the addition of the fluorophoric compound 1-naphthol to the mobile phase. Detection is based on fluorescence enhancement caused by the formation of inclusion complexes. By incorporating 10(-4) M 1-naphthol in the mobile phase, detection limits of 90, 27, 370 and 37 pmol were obtained for beta CD, hydroxypropyl-beta CD, trimethyl-beta CD and dimethyl-beta CD, respectively. The method was applied to the determination of dimethyl-beta CD in urine: the minimum detectable concentration was 0.2 microgram/ml after preconcentration of 10 ml of urine.
Reeuwijk
H J
HJ
Division of Analytical Chemistry, Leiden/Amsterdam Center for Drug Research, Gorlaeus Laboratories, Netherlands.
Irth
H
H
Tjaden
U R
UR
Merkus
F W
FW
van der Greef
J
J
D002853:Chromatography, Liquid / Q000379:methods*; D003505:Cyclodextrins / Q000737:chemistry / Q000652:urine*; D005260:Female; D005456:Fluorescent Dyes; D006801:Humans; D008297:Male; D010865:Pilot Projects; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence; D047392:beta-Cyclodextrins
D003505:Cyclodextrins; D005456:Fluorescent Dyes; D047392:beta-Cyclodextrins; C031215:betadex