Detection of minor adducts in cisplatin-modified DNA by transcription footprinting.
Two DNA restriction fragments containing either a d(GC)5 or a d(TTGCTTGATTAGTTGTGTT) insert were subjected to reaction with cis-diamminedichloroplatinum(II) and were then used as templates for RNA synthesis by T7 RNA polymerase. Within the d(GC)5 insert, interstrand cross-links are preferentially formed. Within the second insert, the reactivity order of the potential binding sites is d(ApG) > d(GpC/GpC) = d(GpA) > d(GpTpG). In the presence of cyanide ions, the adducts are much less stable at the d(GpA) sites than at the d(GpCpG) sites, in double-stranded DNA.
Centre de Biophysique Moléculaire, CNRS, Orléans, France.
M
M
Decoville
A
A
Schwartz
D
D
Locker
M
M
Leng
D001483:Base Sequence; D002945:Cisplatin / Q000032:analysis*; D003486:Cyanides / Q000494:pharmacology; D004247:DNA / Q000032:analysis*; D018736:DNA Adducts; D004249:DNA Damage; D004591:Electrophoresis, Polyacrylamide Gel; D008969:Molecular Sequence Data; D014158:Transcription, Genetic
D003486:Cyanides; D018736:DNA Adducts; C057270:cisplatin-DNA adduct; D004247:DNA; D002945:Cisplatin