Reversible pressure dissociation of R17 bacteriophage. The physical individuality of virus particles.
In the absence of urea, pressures up to 2.5 kbar promote only 10% dissociation of the whole particles of R17 bacteriophage. In the presence of concentrations of urea between 1.0 and 5.0 M, pressure promotes complete, reversible dissociation of the virus particles. At the lower urea concentrations reversible dissociation of R17 virus particles shows no dependence on protein concentration indicating a high degree of heterogeneity of the particles, but higher urea concentrations, 2.5 to 5.0 M, result in progressive restoration of the protein concentration dependence of the pressure dissociation. At still higher urea concentrations, 5.0 to 8.0 M, irreversible dissociation of virus takes place at atmospheric pressure. In contrast, the dissociation of the isolated dimers of the capsid protein was dependent on protein concentration to the extent predicted for a stochastic equilibrium, and dimers were much less stable than the whole virus both to dissociation by pressure or urea. In contradistinction, the reversible whole-virus dissociation observed at urea concentrations below 2.5 M appears to be a typical deterministic equilibrium, without appreciable dynamic exchange between whole particle and subunits during the lengthy experiments. The experiments demonstrate that the "thermodynamic individuality" of the virus particles arises in conformational differences in the assembled viruses, and that there is a direct relation between the stability of the particles and their heterogeneity.
Departamento de Bioquimica, Universidade Federal do Rio de Janeiro, Brazil.
A T
AT
Da Poian
A C
AC
Oliveira
L P
LP
Gaspar
J L
JL
Silva
G
G
Weber
D002213:Capsid / Q000502:physiology; D003090:Coliphages / Q000472:pathogenicity / Q000502:physiology* / Q000648:ultrastructure; D006874:Hydrostatic Pressure; D008854:Microscopy, Electron; D012317:RNA Phages / Q000472:pathogenicity / Q000502:physiology* / Q000648:ultrastructure; D013050:Spectrometry, Fluorescence; D013816:Thermodynamics; D014508:Urea; D014771:Virion / Q000502:physiology
D014508:Urea