Aldosterone stimulated differentiation of mouse 3T3-L1 cells into adipocytes.
We find that 1-10 nM aldosterone can induce differentiation of 3T3-L1 cells into adipose cells as evaluated by microscopic accumulation of fat droplets and quantitative measurement of triglycerides and of glycerol-3-phosphate dehydrogenase, an enzyme specific for adipocyte differentiation. Moreover, the aldosterone antagonist ZK91587 inhibits aldosterone-but not glucocorticoid-mediated differentiation of 3T3-L1 cells. Steroid binding assays with 3T3-L1 cells indicate the presence of specific binding sites for aldosterone. We conclude that there is an aldosterone receptor-mediated pathway for terminal differentiation of 3T3-L1 cells into adipose cells. Receptors for aldosterone have also been found in a variety of cells that do not function to regulate sodium and potassium transport. The aldosterone receptor may have a role in regulation expression of genes involved in differentiation of these cells.
Rondinone
C M
CM
Laboratory of Theoretical and Physical Biology, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892.
Rodbard
D
D
Baker
M E
ME
D016475:3T3 Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D000273:Adipose Tissue / Q000166:cytology*; D000450:Aldosterone / Q000378:metabolism / Q000494:pharmacology*; D000732:Androstanols / Q000494:pharmacology; D000818:Animals; D001667:Binding, Competitive; D002454:Cell Differentiation / Q000187:drug effects; D005993:Glycerolphosphate Dehydrogenase / Q000378:metabolism; D006854:Hydrocortisone / Q000494:pharmacology; D051379:Mice; D011965:Receptors, Glucocorticoid / Q000378:metabolism; D013148:Spironolactone / Q000031:analogs & derivatives / Q000494:pharmacology; D014280:Triglycerides / Q000096:biosynthesis
D000732:Androstanols; D011965:Receptors, Glucocorticoid; D014280:Triglycerides; D013148:Spironolactone; D000450:Aldosterone; C049154:11,17-dihydroxy-6-methyl-17-(1-propynyl)androsta-1,4,6-triene-3-one; C054537:ZK 91587; D005993:Glycerolphosphate Dehydrogenase; D006854:Hydrocortisone