DNA content measurements and an improved idiogram for the Indian muntjac.
The Indian muntjac, an asiatic deer, has the lowest diploid chromosome number among mammals (female 2N = 6; male 2N = 7). Using flow cytometric quantification of propidium iodide-stained cells, we determined the DNA content of muntjac cells to be 94% that of human. This suggests that the muntjac may serve as a model for investigation of karyotypic evolution and rearrangement. In order to facilitate future comparative gene mapping studies, computer-aided analysis of digitized metaphase chromosomes allowed development of a detailed Indian muntjac G-banded idiogram incorporating both ISCN-type nomenclature and quantitative estimates of the size of each band and position.
Department of Obstetrics and Gynecology, University of Maryland, Baltimore 21201.
H P
HP
Levy
R A
RA
Schultz
J V
JV
Ordonez
M M
MM
Cohen
D000818:Animals; D005075:Biological Evolution; D002460:Cell Line; D002871:Chromosome Banding; D004247:DNA / Q000032:analysis*; D003670:Deer / Q000235:genetics*; D005260:Female; D005347:Fibroblasts; D005434:Flow Cytometry; D006801:Humans; D007621:Karyotyping; D008297:Male; D013045:Species Specificity
D004247:DNA