Response to repeated inoculations with Ascaris suum eggs in pigs during the fattening period. II. Specific IgA, IgG, and IgM antibodies determined by enzyme-linked immunosorbent assay.
Experimental trickle inoculations of pigs with low doses (2 x 25 eggs/week) and high doses (2 x 500 eggs/week) of Ascaris suum were followed by enzyme-linked immunosorbent assay (ELISA) during a 14-week period. Three antigens were employed for coating: hatching fluid of embryonated eggs, excretory/secretory antigens from in vitro-cultivated infective larvae (L2/L3-ES), and adult body fluid. Seroconversion times (week 2 for the high-dose group and week 4 for the low-dose group) were essentially identical for the three antigens. The assay employing L2/L3-ES, however, produced significantly higher readings. Specific serum antibody in the IgA and IgG classes showed very similar time courses in both groups. A weak and transient, specific IgM response was recorded in the high-dose group. Mean ELISA responses to L2/L3-ES did not differ significantly between the groups at the termination of the experiment. Specific antibody correlated weakly with the number of liver milk spots recorded at slaughter.
Lind
P
P
Department of Veterinary Microbiology, Royal Veterinary and Agricultural University, Frederiksberg, Denmark.
Eriksen
L
L
Nansen
P
P
Nilsson
O
O
Roepstorff
A
A
D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood / Q000276:immunology*; D000918:Antibody Specificity; D001196:Ascariasis / Q000517:prevention & control / Q000662:veterinary*; D017165:Ascaris suum / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D007070:Immunoglobulin A / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D007075:Immunoglobulin M / Q000276:immunology; D013552:Swine; D013553:Swine Diseases / Q000469:parasitology / Q000517:prevention & control*; D013997:Time Factors; D014612:Vaccines / Q000276:immunology*
D000909:Antibodies, Helminth; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D014612:Vaccines