Analysis of genetic diversity at the arom locus in isolates of Pneumocystis carinii.
The DNA sequences of a portion of the 5-enolpyruvyl shikimate phosphate synthase domain of the arom gene, encoding the pentafunctional AROM protein, were determined from isolates of Pneumocystis carinii from five mammalian host species (rat, human, ferret, rabbit and mouse). High levels of genetic divergence were found among P. carinii derived from different host species, 7-22% at the DNA sequence level, and 7-26% at the derived amino acid sequence level. Two separate and distinct sequences were isolated from infected ferret lungs. Low levels of divergence were seen in human-derived organisms.
Department of Paediatrics, John Radcliffe Hospital, United Kingdom.
S
S
Banerji
E B
EB
Lugli
R F
RF
Miller
A E
AE
Wakefield
D051229:3-Phosphoshikimate 1-Carboxyvinyltransferase; D017088:AIDS-Related Opportunistic Infections / Q000382:microbiology; D019883:Alkyl and Aryl Transferases; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001992:Bronchoalveolar Lavage Fluid / Q000382:microbiology; D017931:DNA Primers; D005289:Ferrets; D005800:Genes, Fungal; D014644:Genetic Variation; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D011010:Pneumocystis / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification*; D011020:Pneumonia, Pneumocystis / Q000150:complications / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D011817:Rabbits; D051381:Rats; D017207:Rats, Sprague-Dawley; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D014166:Transferases / Q000096:biosynthesis / Q000235:genetics*
D017931:DNA Primers; D014166:Transferases; D019883:Alkyl and Aryl Transferases; D051229:3-Phosphoshikimate 1-Carboxyvinyltransferase