Temperature-sensitive sporulation caused by a mutation in the Bacillus subtilis secY gene.
A thermosensitive sporulation mutant of Bacillus subtilis containing a mutation in the secY gene was isolated and characterized. No asymmetric septum specific to the sporulation was detected by electron microscopy at the nonpermissive temperature, indicating that the block occurred at a very early stage of sporulation. Furthermore, competence development in the mutant cell was affected even at the sporulation-proficient temperature. It is assumed that the SecY protein of B. subtilis has multiple roles both in the regulation of spore formation and in stationary-phase-associated phenomena.
Institute of Molecular and Cellular Biosciences, University of Tokyo, Japan.
H
H
Yoshikawa
S M
SM
Jeong
A
A
Hirata
F
F
Kawamura
R H
RH
Doi
H
H
Takahashi
D001412:Bacillus subtilis / Q000235:genetics* / Q000648:ultrastructure; D001426:Bacterial Proteins / Q000235:genetics*; D029968:Escherichia coli Proteins; D006358:Hot Temperature; D009024:Morphogenesis / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D000069816:SEC Translocation Channels; D013171:Spores, Bacterial / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D012333:RNA, Messenger; D000069816:SEC Translocation Channels; C057841:SecY protein, E coli; C080950:Spo0A protein, Bacillus subtilis; D014157:Transcription Factors