Detection of oocysts and IgG antibodies to Cryptosporidium parvum in asymptomatic adult cattle.
Infection by Cryptosporidium was detected in 94 (71.75%) asymptomatic adult cattle from 131 fecal samples examined microscopically. In two cases Cryptosporidium oocysts were observed which were distinctly larger (5.5-6.5 microns x 6.6-7.0 microns) than those we had seen in the majority of feces examined (4.0-4.5 microns x 4.0-4.5 microns) and these specimens were considered to be Cryptosporidium muris; it is possible that the other oocysts should be considered as Cryptosporidium parvum. The seroprevalence of IgG antibodies to Cryptosporidium was 63.35% as detected by indirect fluorescent antibody test (IFAT) and 51.41% by enzyme-linked immunosorbent assay (ELISA). In 27 cases, the presence of IgG antibodies to Cryptosporidium (as tested by IFAT and ELISA) in serum samples was correlated with oocyst excretion.
Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Santiago de Compostela, La Coruña, Spain.
M J
MJ
Lorenzo Lorenzo
E
E
Ares-Mazás
I
I
Villacorta Martínez de Maturana
D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology / Q000469:parasitology*; D003457:Cryptosporidiosis / Q000276:immunology / Q000469:parasitology*; D016785:Cryptosporidium parvum / Q000276:immunology / Q000302:isolation & purification*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005243:Feces / Q000469:parasitology*; D005455:Fluorescent Antibody Technique / Q000662:veterinary; D007074:Immunoglobulin G / Q000097:blood*
D000913:Antibodies, Protozoan; D007074:Immunoglobulin G