Arbitrarily primed polymerase chain reaction of individual Trichinella specimens.
Recently, 5 sibling species and 3 other phenotypes were identified in the genus Trichinella. Single primers of arbitrary nucleotide sequence were used to produce random amplified polymorphic DNA starting from decreasing amounts of Trichinella spiralis and Trichinella britovi DNA. Reproducible amplification products from 30 pg of DNA were obtained using 1 of 6 examined primers. These fragments distinguish between 2 European Trichinella species, T. spiralis, showing a 1,350-bp band, and T. britovi, showing 400- and 1,100-bp bands. The developed procedure allows the characterization of crude DNA preparations of single muscle-stage larvae, avoiding time-consuming passages of parasites in laboratory animals.
I.D.V.G.A.-C.N.R., Milan, Italy.
C
C
Bandi
G
G
La Rosa
M G
MG
Bardin
G
G
Damiani
I
I
de Carneri
E
E
Pozio
D000818:Animals; D001483:Base Sequence; D002335:Carnivora; D004247:DNA / Q000032:analysis*; D004277:DNA, Single-Stranded / Q000737:chemistry; D005819:Genetic Markers; D007814:Larva / Q000145:classification / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D051381:Rats; D013552:Swine; D014234:Trichinella / Q000145:classification* / Q000235:genetics; D017160:Trichinella spiralis / Q000145:classification* / Q000235:genetics
D004277:DNA, Single-Stranded; D005819:Genetic Markers; D004247:DNA