Expression and assembly of the potato virus Y (PVY) coat protein (CP) in Escherichia coli cells.
A clone harboring the full-length cDNA of potato virus Y in a lambda-DASH vector under the control of a T7 promoter was introduced into Escherichia coli carrying the T7-RNA-polymerase gene on a plasmid. The viral coat protein was expressed and the product was of the same size as the corresponding mature protein in infected plants. Immunoelectronmicroscopy of transfected cell extracts revealed virus-like particles, indicating that the proteins involved in its processing and the viral coat protein retained their native activity.
Virus Laboratory, Hebrew University of Jerusalem, Faculty of Agriculture, Rehovot, Israel.
Y
Y
Stram
I
I
Sela
O
O
Edelbaum
E
E
Tanne
M
M
Karchi
H
H
Karchi
D002213:Capsid / Q000737:chemistry / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D015967:Gene Expression Regulation, Viral; D016253:Microscopy, Immunoelectron; D010942:Plant Viruses / Q000235:genetics*; D011198:Solanum tuberosum / Q000382:microbiology; D014162:Transfection