An ELISA assay for murine interleukin-1 beta.
An ELISA assay was developed for murine IL-1 beta (mIL-1 beta) using a polyclonal antibody generated in rabbits. The antibody was purified by affinity chromatography on protein A coupled to Sepharose followed by chromatography on mIL-1 beta coupled to Sepharose. The protein A and affinity purified populations were compared using radiolabeled mIL-1 beta and the results used to develop the conditions for the ELISA. The assay developed is sensitive to pg/ml concentrations of mIL-1 beta, is comparable in sensitivity to one which uses a hamster monoclonal antibody as the capture antibody, and can be used to detect IL-1 beta in peritoneal washings or tissue lysates from either mouse or rat. There is no cross reaction with any cytokine tested. The use of ELISA enhancement kits can increase the resolution at the lower concentration ranges without affecting assay sensitivity. This assay should prove useful for defining the presence and potential role for IL-1 beta in animal models of disease.
DuPont Merck Pharmaceutical Co., Wilmington, DE 19880-0400.
R C
RC
Newton
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R
Dowling
A J
AJ
Daulerio
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S
Culp
D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay; D007375:Interleukin-1 / Q000032:analysis*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011817:Rabbits; D012680:Sensitivity and Specificity
D007375:Interleukin-1