Activated microglia inhibit multiplication of Toxoplasma gondii via a nitric oxide mechanism.
The role of microglia in host defense against Toxoplasma gondii is unknown. In the present study, we investigated the multiplication of T. gondii tachyzoites in murine microglial cell cultures. T. gondii multiplied readily in these cells; multiplication was prevented when microglia were activated with interferon-gamma plus lipopolysaccharide, a treatment that also upregulates nitric oxide (NO) synthase activity. Simultaneous treatment of microglial cell cultures with activation signals and the NO synthase inhibitor NG-monomethyl-L-arginine (NGMA) prevented the antitoxoplasmic activity. Transmission electron microscopic analysis demonstrated degenerative tachyzoites in activated microglia but not in control or NGMA groups. These findings support the view that the host defense function of activated microglia against T. gondii involves generation of the free radical NO.
Chao
C C
CC
Neuroimmunobiology and Host Defense Laboratory, Minneapolis Medical Research Foundation, Minnesota 55404.
Anderson
W R
WR
Hu
S
S
Gekker
G
G
Martella
A
A
Peterson
P K
PK
D000818:Animals; D002478:Cells, Cultured; D007371:Interferon-gamma / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008262:Macrophage Activation; D008264:Macrophages / Q000276:immunology* / Q000378:metabolism / Q000469:parasitology / Q000648:ultrastructure; D051379:Mice; D008807:Mice, Inbred BALB C; D008854:Microscopy, Electron; D009457:Neuroglia / Q000276:immunology* / Q000378:metabolism / Q000469:parasitology / Q000648:ultrastructure; D009569:Nitric Oxide / Q000378:metabolism*; D014122:Toxoplasma / Q000254:growth & development* / Q000276:immunology
D008070:Lipopolysaccharides; D009569:Nitric Oxide; D007371:Interferon-gamma