Modified polyvinyl alcohol-benzoporphyrin derivative conjugates as phototoxic agents.
Photosensitizing and biodistribution characteristics of a photosensitizer (benzoporphyrin derivative, monoacid ring A; BPD) conjugated to a macromolecule (modified polyvinyl alcohol; M-PVA, molecular weight = 10,000) were tested in vitro and in vivo. Modified PVA was loaded with BPD at molar ratios 1:12, 1:25, 1:50, 1:75 and 1:100. Most of the work was carried out with a conjugate having a 1:25 molar ratio. In vitro photosensitization was tested using A549 (human lung carcinoma), A432 (human epidermoid carcinoma) and P815 (mastocytoma of DBA/2 mice) cell lines. Photosensitization of M1 (rhabdomyosarcoma of DBA/2 mice) tumors was tested in an in vivo/in vitro assay, in which tumor-bearing mice were injected intravenously with free or conjugated 3H-BPD and 3 h later light activation of tumor cells was carried out in vitro. Biodistribution studies were carried out using M1 tumor-bearing DBA/2 mice and 3H-BPD either free or conjugated to M-PVA. The results of these studies showed that the conjugation of BPD to M-PVA resulted in the formation of a macromolecular photosensitizer that retained full photosensitizing activity of the photosensitizer molecules and at the same time gained new characteristics, advantageous for photodynamic treatment, especially in vivo. In vitro M-PVA-BPD conjugates were at least as efficient in photosensitization of tumor cells as an equivalent number of free BPD molecules, both in the presence and in the absence of serum. Although the biodistribution was in general comparable to free BPD, the conjugate (1:25) reached slightly higher levels in the blood, kidney, lung and spleen, and lower levels in the liver, brain, skin and muscle in comparison with free BPD.(ABSTRACT TRUNCATED AT 250 WORDS)
Department of Microbiology, University of British Columbia, Vancouver, Canada.
N
N
Davis
D
D
Liu
A K
AK
Jain
S Y
SY
Jiang
F
F
Jiang
A
A
Richter
J G
JG
Levy
D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008027:Light; D008175:Lung Neoplasms; D008297:Male; D012515:Mast-Cell Sarcoma; D051379:Mice; D008811:Mice, Inbred DBA; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000493:pharmacokinetics / Q000627:therapeutic use* / Q000633:toxicity*; D011142:Polyvinyl Alcohol / Q000493:pharmacokinetics / Q000627:therapeutic use / Q000633:toxicity; D011166:Porphyrins / Q000493:pharmacokinetics / Q000627:therapeutic use* / Q000633:toxicity*; D012208:Rhabdomyosarcoma / Q000188:drug therapy*; D013329:Structure-Activity Relationship; D014018:Tissue Distribution; D014407:Tumor Cells, Cultured; D014466:Ultraviolet Rays
D017319:Photosensitizing Agents; D011166:Porphyrins; C504224:benzoporphyrin D; D011142:Polyvinyl Alcohol