[A new method for the isolation of peripheral blood eosinophils using flow cytometry (FCM)].
Human eosinophils are known to exhibit autofluorescence, which allows the preparation of highly enriched eosinophil suspensions from peripheral blood of normal donors and bronchoaveolar lavage fluid, using flow cytometry (FCM). The sorted eosinophils were morphologically normal by light microscopy, the purity was more than 90% and the viability was more than 90%. The function of purified eosinophils was evaluated by chemotactic activity to platelet activating factor (PAF:10(-7) M) and recombinant human interleukin-5 (rhIL-5:10(3) ng/ml). Chemotactic activity was greater in purified eosinophils from patients with asthma than from normal subjects. Flow cytometric separation and analysis of eosinophils should thus, facilitate future studies of eosinophil heterogeneity and function.
Department of Internal Medicine, Kochi Municipal Central Hospital.
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Kawada
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Takahashi
D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D002469:Cell Separation / Q000379:methods*; D002499:Centrifugation, Density Gradient; D002634:Chemotaxis, Leukocyte; D004804:Eosinophils / Q000166:cytology* / Q000502:physiology; D005434:Flow Cytometry; D006801:Humans; D010972:Platelet Activating Factor
D010972:Platelet Activating Factor