The reductive pentose phosphate cycle for photosynthetic CO2 assimilation: enzyme modulation.
The reductive pentose phosphate cycle (Benson-Calvin cycle) is the main biochemical pathway for the conversion of atmospheric CO2 to organic compounds. Two unique systems that link light-triggered events in thylakoid membranes with enzyme regulation are located in the soluble portion of chloroplasts (stroma): the ferredoxin-thioredoxin system and ribulose 1,5-bisphosphate carboxylase/oxygenase-Activase (Rubisco-Activase). The ferredoxin-thioredoxin system (ferredoxin, ferredoxin-thioredoxin reductase, and thioredoxin) transforms native (inactive) glyceraldehyde-3-P dehydrogenase, fructose-1,6-bisphosphatase, sedoheptulose-1,7-bisphosphatase, and phosphoribulokinase to catalytically competent forms. However, the comparison of enzymes reveals the absence of common amino acid sequences for the action of reduced thioredoxin. Thiol/disulfide exchanges appear as the underlying mechanism, but chloroplast metabolites and target domains make the activation process peculiar for each enzyme. On the other hand, Rubisco-Activase facilitates the combination of CO2 with a specific epsilon-amino group of ribulose 1,5-bisphosphate carboxylase/oxygenase and the subsequent stabilization of the carbamylated enzyme by Mg2+, in a reaction that depends on ATP and ribulose 1,5-bisphosphate. Most of these studies were carried out in homogeneous solutions; nevertheless, a growing body of evidence indicates that several enzymes of the cycle associate either with thylakoid membranes or with other proteins yielding supra-molecular complexes in the chloroplast.
Wolosiuk
R A
RA
Instituto de Investigaciones Bioquímicas, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina.
Ballicora
M A
MA
Hagelin
K
K
D000595:Amino Acid Sequence; D002245:Carbon Dioxide / Q000378:metabolism*; D002736:Chloroplasts / Q000201:enzymology*; D008969:Molecular Sequence Data; D010427:Pentose Phosphate Pathway; D010788:Photosynthesis; D013879:Thioredoxins / Q000378:metabolism
D002245:Carbon Dioxide; D013879:Thioredoxins