Effect of beta propiolactone on specific antibody measurements by ELISA.
Serum samples from 30 HIV seronegative patients were treated with beta propiolactone (BPL) to determine whether BPL interfaces with ELISA for specific antibodies against protein and carbohydrate antigens. BPL had no discernible effect on specific antibody measurements by ELISA. With the measuring need for specific antibody measurements in the management of HIV seropositive patients, it is reassuring that this laboratory safety measure does not impair the reliability of results.
Department of Immunology, John Radcliffe Hospital, Oxford.
A E
AE
Agbarakwe
S A
SA
Misbah
H
H
Griffiths
H M
HM
Chapel
D000907:Antibodies, Bacterial / Q000032:analysis / Q000187:drug effects*; D016667:Bacterial Capsules; D001428:Bacterial Vaccines / Q000276:immunology; D004168:Diphtheria Toxoid / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D015658:HIV Infections / Q000276:immunology*; D018073:Haemophilus Vaccines; D006801:Humans; D022242:Pneumococcal Vaccines; D011135:Polysaccharides, Bacterial / Q000276:immunology; D011420:Propiolactone / Q000494:pharmacology*; D013745:Tetanus Toxoid / Q000276:immunology
D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; D004168:Diphtheria Toxoid; D018073:Haemophilus Vaccines; C044237:Haemophilus influenzae type b polysaccharide vaccine; D022242:Pneumococcal Vaccines; D011135:Polysaccharides, Bacterial; D013745:Tetanus Toxoid; D011420:Propiolactone