Visualization of ultraviolet light-induced thymine dimers in DNA by immunoelectron microscopy.
To see the damage of DNA due to ultraviolet-B more distinctly, immunoelectron microscopic studies using a monoclonal antibody against cyclobutane-type thymine dimers were performed. As a result, we could detect the existence of thymine dimers on human genomic DNA and pUC18 plasmid DNA visually. This technique can be useful to locate the photoproducts formed on DNA.
Udono
M U
MU
Department of Dermatology, Nagasaki University School of Medicine, Japan.
Hori
M
M
Hirayama
M
M
Yoshida
H
H
Suematsu
T
T
Suematsu
T
T
Mori
T
T
Matsunaga
T
T
Mizuno
T
T
Nikaido
O
O
D004247:DNA / Q000097:blood / Q000528:radiation effects* / Q000648:ultrastructure; D006801:Humans; D007962:Leukocytes; D016253:Microscopy, Immunoelectron; D010957:Plasmids; D011740:Pyrimidine Dimers / Q000032:analysis*; D014466:Ultraviolet Rays
D011740:Pyrimidine Dimers; D004247:DNA