Immunological studies using monoclonal antibodies for detection of low dalton marine toxins.
The general methodology employed in the development of an immunological assay system is initially described. These include: (a) the methods and reagents used for coupling low dalton epitopes; (b) selection of appropriate immunogenic carriers; (c) mode of administration and choice of animal; and (d) the method of assessing the antibody produced. The background and methodology, using ciguatoxin as a model, are presented. The stick enzyme immunoassay (S-EIA) has proven of value to evaluate implicated fish for verification of ciguatera poisoning and for screening of fish associated with ciguatera prior to consumption. No false negatives have occurred with the stick enzyme immunoassay system to date.
Hokama
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Department of Pathology, John A. Burns School of Medicine, University of Hawaii, Honolulu 96822.
D000818:Animals; D036841:Ciguatera Poisoning; D002922:Ciguatoxins / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005399:Fishes; D005506:Food Contamination; D005517:Foodborne Diseases / Q000209:etiology*; D006801:Humans; D008387:Marine Toxins / Q000032:analysis* / Q000506:poisoning; D011863:Radioimmunoassay / Q000379:methods
D008387:Marine Toxins; D002922:Ciguatoxins