Up-regulation of IL-6-receptors by IL-3 on a plasma cell leukaemia cell line which proliferates dependently on both IL-3 and IL-6.
In this report we show that IL-3 up-regulates the expression of IL-6-receptors on a plasma cell leukaemia cell line which proliferates in response to both IL-3 and IL-6. Quantitative binding studies and Scatchard analysis revealed that HSM-2.3 has a single class of high-affinity IL-6 binding sites (200/cell, dissociation constant at the equilibrium 1.43 x 10(-11) M). After stimulation with IL-3, HSM-2.3 has 1200 binding sites. IL-6 receptor mRNA expression was detected in HSM-2.3 only after stimulation with IL-3.
Laboratory of Pathology, Cancer Institute, Hokkaido University School of Medicine, Sapporo, Japan.
M
M
Kobayashi
J
J
Tanaka
M
M
Imamura
S
S
Maeda
H
H
Iwasaki
M
M
Tanaka
Y
Y
Tsudu
K
K
Sakurada
T
T
Miyazaki
D001483:Base Sequence; D006801:Humans; D007377:Interleukin-3 / Q000276:immunology*; D015850:Interleukin-6 / Q000235:genetics / Q000276:immunology*; D007952:Leukemia, Plasma Cell / Q000276:immunology*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis; D011971:Receptors, Immunologic / Q000032:analysis* / Q000235:genetics; D019947:Receptors, Interleukin-6; D014407:Tumor Cells, Cultured / Q000276:immunology; D015854:Up-Regulation
D007377:Interleukin-3; D015850:Interleukin-6; D012333:RNA, Messenger; D011971:Receptors, Immunologic; D019947:Receptors, Interleukin-6