Expression of protein kinase C isozymes in rat glial cell line.
To study the presence and distribution of various isozymes of PKC in glial cells, immunofluorescent studies were performed on a cortical glial cell line cultured derived from rat cerebral cortex and analyzed by a highly sensitive automated laser cytometric device, ACAS-570 (Adherent Cell Analysis and Sorting). The antibodies tested were against alpha, beta, gamma and epsilon isozymes of PKC, and the catalytic domain (cd), i.e. antibody against the cd to all isozymes of PKC. The data indicate that rat cortical glial cell line expresses at least three isozymes of PKC, epsilon, beta, and alpha, the degree of intensity of immunofluorescence being epsilon > beta > alpha. The immunofluorescence for PKC-cd was the most intense.
Department of Pathology, Michigan State University, East Lansing 48824.
K
K
Kumar
B
B
Kim
H
H
Rupp
B
B
Madhukar
D000818:Animals; D000911:Antibodies, Monoclonal; D002460:Cell Line; D002540:Cerebral Cortex / Q000166:cytology / Q000201:enzymology*; D005455:Fluorescent Antibody Technique; D007527:Isoenzymes / Q000032:analysis*; D007834:Lasers; D009457:Neuroglia / Q000201:enzymology*; D011493:Protein Kinase C / Q000032:analysis*; D051381:Rats; D011916:Rats, Inbred F344
D000911:Antibodies, Monoclonal; D007527:Isoenzymes; D011493:Protein Kinase C