A new human guanylate cyclase-activating peptide (GCAP-II, uroguanylin): precursor cDNA and colonic expression.
We have amplified, cloned, and sequenced 583 bp GCAP-II/uroguanylin-specific cDNA from human colon cDNA first strand. The cDNA codes for a putative 112 amino-acid precursor protein including the sequence of uroguanylin and GCAP-II. Northern blot hybridization revealed a high level expression of the GCAP-II gene in human colon, but not in the kidney. This expression of GCAP-II indicates a pivotal role in cGMP-mediated functions of the colon.
Lower Saxony Institute for Peptide Research, Hannover, Germany.
O
O
Hill
Y
Y
Cetin
A
A
Cieslak
H J
HJ
Mägert
W G
WG
Forssmann
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D002135:Calcium-Binding Proteins / Q000235:genetics*; D003106:Colon / Q000378:metabolism*; D006152:Cyclic GMP / Q000378:metabolism; D018076:DNA, Complementary; D051604:Guanylate Cyclase-Activating Proteins; D006801:Humans; D008969:Molecular Sequence Data; D045265:Natriuretic Peptides; D010455:Peptides / Q000235:genetics*; D011498:Protein Precursors / Q000235:genetics*; D012322:RNA Precursors / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D017422:Sequence Analysis, DNA; D014018:Tissue Distribution
D002135:Calcium-Binding Proteins; D018076:DNA, Complementary; C496739:GUCA1B protein, human; D051604:Guanylate Cyclase-Activating Proteins; D045265:Natriuretic Peptides; D010455:Peptides; D011498:Protein Precursors; D012322:RNA Precursors; D012333:RNA, Messenger; C084362:uroguanylin; D006152:Cyclic GMP