Sequence analysis of cloned human satellite DNAs reveals nonrandom variations.
We have cloned, from the genome of HeLa cells, related 1.8-kb highly repetitive EcoRI satellite II (and III) DNA family members that displayed a high degree of TaqI and HinfI variations. Comparative sequence analysis of these cloned DNAs suggests that highly repetitive satellite DNAs may have evolved and diversified from the rapid amplification of a pentameric unit (5' TTCCA 3'), by processes including random and non-random mutations. Base conservation, as well as mutational hotspots, were found associated with these related satellite II and III DNAs. The accumulation of C to G transversions within the pentamers, generating TaqI and HinfI sites, as well as C to T transitions at CpN dinucleotides, appear to be responsible for much of the microheterogeneity observed between related satellite II and III DNA family members.
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Department of Microbiology and Immunology, McGill University, Montréal, Que., Canada.
DuBow
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MS
D001483:Base Sequence; D003001:Cloning, Molecular; D004276:DNA, Satellite; D015252:Deoxyribonucleases, Type II Site-Specific; D014644:Genetic Variation; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D017421:Sequence Analysis
D004276:DNA, Satellite; D015252:Deoxyribonucleases, Type II Site-Specific; C032229:GANTC-specific type II deoxyribonucleases; C027046:TCGA-specific type II deoxyribonucleases