Different expression of procoagulant activity in human cancer cells cultured "in vitro" or in cells isolated from human tumor tissues.
We studied in a homologous system the procoagulant activity of human tumor cells cultured "in vitro" (1402 primary melanoma, Me 7110/2 metastatic melanoma, Hep G2 hepatoma and GLC1 small cell lung carcinoma) or of cells freshly isolated from different human tumor tissues. Tumor cells cultured "in vitro" possessed and released a factor VII dependent procoagulant activity, which was inhibited by concanavalin A and unaffected by iodoacetamide or HgCl2. The activity released by the cells of metastatic melanoma was higher than that released by the cells of the primary tumor. On the contrary, cancer cells isolated from tumor tissues possessed and released a factor VII independent activity which was inhibited by iodoacetamide of HgCl2 and was not modified by concanavalin A. Therefore, different methods for the preparation of tumor cell suspensions have to be used for the study of tumor procoagulants, since their expression depends very largely on the source of tumor cells. Furthermore, cultured human tumor cells are not an appropriate model for the "in vivo" procoagulant effect of tumor cells.
Department of Internal Medicine, University of Pavia, Italy.
M
M
Zucchella
L
L
Pacchiarini
F
F
Tacconi
A
A
Saporiti
G
G
Grignani
D001779:Blood Coagulation Factors / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000378:metabolism; D003208:Concanavalin A / Q000494:pharmacology; D003546:Cysteine Endopeptidases / Q000096:biosynthesis* / Q000378:metabolism; D005167:Factor VII / Q000494:pharmacology; D005170:Factor X / Q000494:pharmacology; D006801:Humans; D007460:Iodoacetamide / Q000494:pharmacology; D008545:Melanoma / Q000378:metabolism / Q000473:pathology; D008627:Mercuric Chloride / Q000494:pharmacology; D009363:Neoplasm Proteins; D009369:Neoplasms / Q000473:pathology*; D014411:Neoplastic Stem Cells / Q000378:metabolism*; D014407:Tumor Cells, Cultured / Q000378:metabolism*
D001779:Blood Coagulation Factors; D009363:Neoplasm Proteins; D003208:Concanavalin A; D008627:Mercuric Chloride; D005167:Factor VII; D005170:Factor X; D003546:Cysteine Endopeptidases; C030910:cancer procoagulant; D007460:Iodoacetamide