Characterization of an eosinophilic leukemia cell differentiation factor (ELDF) produced by a human T cell leukemia cell line, HIL-3.
An adult T cell leukemia cell line, HIL-3, constitutively secretes a factor which induces the phenotypical and functional eosinophilic differentiation of a human eosinophilic leukemia cell line, EoL-1. Biochemical characteristics of the factor, termed eosinophilic leukemia cell differentiation factor (ELDF), were examined. ELDF was precipitated by 35 to 65% saturated ammonium sulfate from the culture supernatants of HIL-3 cells (HIL-3 sup). ELDF was eluted in a peak corresponding to a molecular weight of 30 to 40 kd by gel filtration. Isoelectric focusing in the Rotofor showed that ELDF had isoelectric points of 5 to 6. ELDF was trypsin-sensitive and stable to heat treatment at 65 degrees C for 30 minutes but labile at 80 degrees C or pH lower than 3. Half of the activity adhered to lentil-lectin but not to Con-A, indicating that a part of ELDF is glycoprotein with an N-linked carbohydrate moiety, which did not seem to be essential for ELDF activity. The biochemical characteristics of ELDF and blocking experiments using cytokine-specific neutralizing antibodies suggest that ELDF is different from gamma-interferon (IFN-gamma), tumor necrosis factor-alpha (TNF-alpha), granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukin-5 (IL-5) and interleukin-2 (IL-2), which may exist in HIL-3 sup, and that ELDF may be a previously unrecognized leukemia differentiation factor.
Department of Pediatrics, Faculty of Medicine, Kyoto University, Japan.
Y
Y
Ohshima
M
M
Morita
M
M
Hirashima
K J
KJ
Mori
H
H
Akutagawa
K
K
Katamura
M
M
Mayumi
H
H
Mikawa
D002454:Cell Differentiation / Q000187:drug effects*; D002847:Chromatography, Agarose; D002850:Chromatography, Gel; D016895:Culture Media, Serum-Free / Q000032:analysis / Q000494:pharmacology; D016207:Cytokines / Q000737:chemistry* / Q000378:metabolism*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000032:analysis / Q000494:pharmacology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007371:Interferon-gamma / Q000032:analysis / Q000494:pharmacology; D015848:Interleukin-5 / Q000032:analysis / Q000494:pharmacology; D007525:Isoelectric Focusing; D015472:Leukemia, Eosinophilic, Acute; D015458:Leukemia, T-Cell / Q000378:metabolism* / Q000473:pathology*; D008970:Molecular Weight; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis / Q000494:pharmacology
D016895:Culture Media, Serum-Free; D016207:Cytokines; D015848:Interleukin-5; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma; D016178:Granulocyte-Macrophage Colony-Stimulating Factor