The reduction of the inhibitory effect of aluminum on Na+ efflux in barnacle muscles fibers by preinjecting phosphate.
The object of the present study was to test the hypothesis that the pre-enrichment of single muscle fibers from the barnacle Balanus nubilus with inorganic phosphate may protect the basal Na efflux from the inhibitory effect of Al injection. This approach was adopted in the light of evidence that the preinjection of ATP fails to stop the Na efflux in unpoisoned fibers from falling following the injection of Al. The results of the experiments are as follows: (i) Preinjection of K2HPO4 into unpoisoned fibers reduces the magnitude of the inhibitory effect on the basal Na efflux of injected Al in a dose-dependent manner but fails to completely stop it from occurring. (ii) Injection of K2HPO4 following Al into unpoisoned fibers fails to arrest or reverse the inhibitory effect of injected Al. (iii) Injection of K2HPO4 in a concentration as high as 0.5 M is without effect on the course of the basal Na efflux. (iv) Injection of K2HPO4 into ouabain-poisoned fibers fails to stop Al from stimulating the ouabain-insensitive Na efflux. Injection of K2HPO4 following peak stimulation by injecting Al is also without effect. (v) Injection of K2HPO4 in a concentration as high as 0.5 M is without effect on the course of the ouabain-insensitive Na efflux. Collectively, the results obtained with unpoisoned 'hypersensitive' fibers are consistent with the view that a significant fraction of the injected inorganic phosphate binds Al3+, and hence protects the basal Na efflux from the untoward action of Al3+.
Bittar
E E
EE
Department of Physiology, University of Wisconsin, Madison 53706.
Huang
Y P
YP
D000535:Aluminum / Q000494:pharmacology*; D000077410:Aluminum Chloride; D017607:Aluminum Compounds; D000818:Animals; D002712:Chlorides; D010042:Ouabain; D010710:Phosphates / Q000494:pharmacology*; D011188:Potassium; D017680:Potassium Compounds; D012964:Sodium / Q000378:metabolism*; D001468:Thoracica / Q000187:drug effects*
D017607:Aluminum Compounds; D002712:Chlorides; D010710:Phosphates; D017680:Potassium Compounds; D000077410:Aluminum Chloride; D010042:Ouabain; D012964:Sodium; C013216:potassium phosphate; D000535:Aluminum; D011188:Potassium