Novel instrumentation and biomedical applications of very near infrared fluorescence.
A high wavelength fluorescent probe, Nile Red, was added to four proteins, viz., bovine albumin, alpha 1-acid glycoprotein, beta-lactoglobulin and ovomucoid. Nile Red showed an enhancement in fluorescence and a shift in emission wavelength, suggesting it was bonding hydrophobically to these proteins. Drug displacement of Nile Red from alpha 1-acid glycoprotein was achieved with both D,L-propranolol and flufenamic acid, showing that the binding site is less electrostatic and more hydrophobic in nature. In order to monitor these interactions, a simple spectrofluorimeter was constructed from solid-state components; the sensitivity of this instrument compared well with that of standard laboratory spectrofluorimeters.
Loughborough University of Technology, Leicestershire, UK.
M B
MB
Brown
T E
TE
Edmonds
J N
JN
Miller
D P
DP
Riley
N J
NJ
Seare
D001665:Binding Sites; D001798:Blood Proteins / Q000378:metabolism*; D005439:Flufenamic Acid / Q000378:metabolism; D005456:Fluorescent Dyes; D007782:Lactoglobulins / Q000378:metabolism; D009961:Orosomucoid / Q000378:metabolism; D010059:Ovomucin / Q000378:metabolism; D010078:Oxazines / Q000378:metabolism*; D011433:Propranolol / Q000378:metabolism; D012710:Serum Albumin, Bovine / Q000378:metabolism; D013050:Spectrometry, Fluorescence / Q000295:instrumentation / Q000379:methods
D001798:Blood Proteins; D005456:Fluorescent Dyes; D007782:Lactoglobulins; D009961:Orosomucoid; D010078:Oxazines; D012710:Serum Albumin, Bovine; D010059:Ovomucin; D005439:Flufenamic Acid; D011433:Propranolol; C044808:nile red