The status of the surface enzyme guanidinobenzoatase on mature epithelial cells in sputum cell monolayers.
Mature epithelial cells derived from the sputum of normal healthy adults lack GB, while the epithelial cells of sputum collected from patients with lung tumours contain a spectrum of epithelial cells with active GB, some of which are clearly tumour cells, based upon their morphology. The presence of abnormal epithelial cells was confirmed by the cytologist whilst observing the fluorescent stained cells and later by independent cytological analysis of these same cells employing conventional stains.
Department of Biochemistry and Molecular Biology, School of Biological Sciences, University of Manchester, U.K.
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Steven
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Payne
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Palcic
D000328:Adult; D002265:Carboxylic Ester Hydrolases / Q000032:analysis*; D010450:Endopeptidases; D004847:Epithelial Cells; D004848:Epithelium / Q000201:enzymology; D005456:Fluorescent Dyes; D006801:Humans; D008168:Lung / Q000166:cytology; D008175:Lung Neoplasms / Q000175:diagnosis / Q000201:enzymology / Q000473:pathology; D008565:Membrane Proteins / Q000032:analysis*; D013183:Sputum / Q000166:cytology / Q000201:enzymology*
D005456:Fluorescent Dyes; D008565:Membrane Proteins; D002265:Carboxylic Ester Hydrolases; C037288:guanidinobenzoate esterase; D010450:Endopeptidases