Cholesteryl ester accumulation in macrophages treated with oxidized low density lipoprotein.
The ability of CuSO4- and hypochlorite-oxidized LDL to promote cholesterol accumulation in macrophages was examined. Both CuSO4- and hypochlorite-oxidized LDL were rapidly metabolized by mouse peritoneal macrophages to a level approximately 10 times that observed for native LDL and both modified lipoproteins increased the accumulation of unesterified cholesterol. However when each modified lipoprotein was incubated with macrophages for 40h, only hypochlorite-oxidized LDL produced significant accumulation of cholesteryl esters, with levels approaching 85 micrograms/mg cell protein. This finding was verified by nile red staining. The cholesteryl ester content of cupric sulfate-modified LDL was found to be significantly decreased when compared to either native or hypochlorite-modified LDL promotes massive cholesteryl ester accumulation because the cholesteryl ester content of the LDL particle is preserved.
University of Georgia, College of Pharmacy, Department of Pharmacology and Toxicology, Athens 30602, USA.
B H
BH
Ryu
F W
FW
Mao
P
P
Lou
R L
RL
Gutman
P
P
Greenspan
D000818:Animals; D002788:Cholesterol Esters / Q000737:chemistry* / Q000378:metabolism*; D003300:Copper / Q000737:chemistry; D019327:Copper Sulfate; D005260:Female; D005487:Foam Cells / Q000502:physiology; D006801:Humans; D006997:Hypochlorous Acid / Q000737:chemistry; D008077:Lipoproteins, LDL / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D017737:Macrophages, Peritoneal / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D010084:Oxidation-Reduction
D002788:Cholesterol Esters; D008077:Lipoproteins, LDL; D006997:Hypochlorous Acid; D003300:Copper; D019327:Copper Sulfate