Reversal of inhibition of reactive oxygen species on respiratory burst of macrophages by polysaccharide from Coriolus versicolor.
Using a luminol-dependent, chemiluminescence assay we found tert-butylhydroperoxide to be a strong inhibitor of the respiratory burst of mouse peritoneal macrophages. However, the inhibition of respiratory burst induced by tert-butylhydroperoxide could be prevented after the interperitoneal injection of polysaccharide from Coriolus versicolor (PSK). Further investigation showed that glutathione peroxidase activity was markedly elevated in PSK-treated macrophages. After incubation with tert-butylhydroperoxide, higher activity of glutathione peroxidase was maintained in PSK-treated macrophages. These results suggest that the immunological function of macrophages is related to the activity of glutathione peroxidase. The non-specific immunopolysaccharide might protect macrophages from the damage induced by reactive oxygen species by enhancing antioxidative capacity.
Department of Biochemistry, First Medical College of PLA, Guang Zhou, P.R.C.
L
L
Jun
Z
Z
Mei
C
C
Yuan
D000818:Animals; D005979:Glutathione Peroxidase / Q000378:metabolism; D007155:Immunologic Factors / Q000494:pharmacology*; D015227:Lipid Peroxidation; D008264:Macrophages / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D008297:Male; D051379:Mice; D010545:Peroxides / Q000633:toxicity; D011509:Proteoglycans / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000633:toxicity*; D016897:Respiratory Burst / Q000187:drug effects*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D020122:tert-Butylhydroperoxide
D007155:Immunologic Factors; D010545:Peroxides; D011509:Proteoglycans; D017382:Reactive Oxygen Species; C010770:polysaccharide-K; D020122:tert-Butylhydroperoxide; D005979:Glutathione Peroxidase; D013755:Tetradecanoylphorbol Acetate