Enhancing effect of interleukin-2 on production of parathyroid hormone-related protein by adult T-cell leukemia cells.
Leukemic cells from patients with adult T-cell leukemia (ATL) can produce a calcium-regulating protein, parathyroid hormone-related protein (PTHrP). Moreover, it has been reported that ATL cells produce some cytokines besides PTHrP and that these cells respond to the T-cell growth factors, interleukin-2 (IL-2) and interleukin-4 (IL-4). To elucidate whether PTHrP produced by ATL cells is regulated by IL-2 or IL-4, we investigated the in vitro effects of IL-2 and IL-4 on the release of PTHrP. IL-2 increased the release of PTHrP into the conditioned medium from leukemic cells in some, but not all, ATL patients; however, IL-4 did not affect the PTHrP release. PTHrP messenger RNA (mRNA) levels were increased in ATL cells cultured in the presence of IL-2. These data suggest that IL-2 plays a role in the regulation of hypercalcemia by enhancing the production of PTHrP in ATL patients.
First Department of Internal Medicine, University of Occupational and Environmental Health, School of Medicine, Kitakyushu.
N
N
Mori
K
K
Ohsumi
S
S
Murakami
A
A
Wake
F
F
Shirakawa
I
I
Morimoto
S
S
Oda
S
S
Eto
D000368:Aged; D000369:Aged, 80 and over; D002455:Cell Division / Q000187:drug effects; D003470:Culture Media; D005260:Female; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology*; D015847:Interleukin-4 / Q000494:pharmacology; D007700:Kinetics; D015458:Leukemia, T-Cell / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000378:metabolism; D044162:Parathyroid Hormone-Related Protein; D014176:Protein Biosynthesis; D011506:Proteins / Q000378:metabolism; D013268:Stimulation, Chemical; D014407:Tumor Cells, Cultured
D003470:Culture Media; D007376:Interleukin-2; D009363:Neoplasm Proteins; C470121:PTHLH protein, human; D044162:Parathyroid Hormone-Related Protein; D011506:Proteins; D015847:Interleukin-4