Development of an enzyme-linked immunosorbent assay method for typing and quantitation of Klebsiella pneumoniae lipopolysaccharide: application to serotype O1.
We describe a method for the typing and quantitation of Klebsiella pneumoniae serotype O1 lipopolysaccharide (LPS) based on inhibition in an enzyme-linked immunosorbent assay of a reaction of known O1 LPS antigen and anti-O1 antibody by unknown LPS extracts. Serotype O1 was found in 32% of the 124 K. pneumoniae clinical isolates tested, showing that this serotype is frequent among the eight O serotypes which have been described previously.
Albertí
S
S
Universidad de las Islas Baleares, Palma de Mallorca, Spain.
Hernández-Allés
S
S
Gil
J
J
Reina
J
J
Martínez-Beltrán
J
J
Camprubí
S
S
Tomás
J M
JM
Benedí
V J
VJ
D015373:Bacterial Typing Techniques / Q000706:statistics & numerical data; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods* / Q000706:statistics & numerical data; D005069:Evaluation Studies as Topic; D006801:Humans; D007711:Klebsiella pneumoniae / Q000737:chemistry / Q000145:classification* / Q000302:isolation & purification; D008070:Lipopolysaccharides / Q000032:analysis / Q000145:classification*; D012680:Sensitivity and Specificity; D012703:Serotyping
D008070:Lipopolysaccharides