Experimental studies on in vitro maturation of porcine oocytes. II. Effects of estradiol-17 beta and progesterone.
The simple compounded semi-defined Whitten's medium was supplemented with estradiol-17 beta and progesterone to examine whether they influence the nuclear maturation of porcine cumulus-oocyte complexes. Addition of estradiol-17 beta at concentrations of 1.0 microgram/ml and 10.0 micrograms/ml significantly inhibited the nuclear maturation in comparison to the control group. No significant inhibition of estradiol-17 beta was observed at concentration of 0.1 microgram/ml with respect to the control. After addition of synchronous or subsequent (12 h after the incubation with estradiol-17 beta) supplementation of progesterone at concentration of 1 microgram/ml, maturation rates were as high as control. So progesterone overcame the inhibitory effect of estradiol-17 beta.
Eroglu
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Uludag Universitesi, Veteriner Fakültesi Klinikleri, Bursa, Turkey.
D000818:Animals; D003470:Culture Media; D004958:Estradiol / Q000494:pharmacology*; D008540:Meiosis / Q000187:drug effects; D009865:Oocytes / Q000166:cytology* / Q000187:drug effects; D011374:Progesterone / Q000494:pharmacology*; D013552:Swine / Q000502:physiology*
D003470:Culture Media; D011374:Progesterone; D004958:Estradiol