1H NMR spectroscopic studies of lipid extracts from human fatty liver.
Lipid extracts of biopsy samples from normal and non-alcohol-induced fatty human liver were studied by 1H-NMR at 200 MHz. Spectra of the lipid extracts from 10 mg samples were obtained in 6 min with routine acquisition parameters and allowed the calculation of the phosphatidylcholine to total fatty acyl chain ratio, the cholesterol to total fatty acyl chain ratio, the average fatty acyl chain length, the unsaturation ratio and the acylated glycerol to total fatty acyl chain ratio. The data suggest that lipids with a higher ratio of de novo synthesized fatty acyl chains are stored in non-alcohol-induced fatty liver. NMR lipid analysis appears to be a reliable method for the rapid assessment of hepatic lipid composition on bioptic specimens.
Department of Biochemistry, Biophysics and Macromolecular Chemistry, University of Trieste, Italy.
P
P
Pollesello
F
F
Masutti
L S
LS
Crocè
R
R
Toffanin
O
O
Eriksson
S
S
Paoletti
K
K
Höckerstedt
C
C
Tiribelli
D001706:Biopsy; D005227:Fatty Acids / Q000032:analysis; D005234:Fatty Liver / Q000378:metabolism* / Q000473:pathology; D005260:Female; D005989:Glycerides / Q000032:analysis; D006801:Humans; D006859:Hydrogen; D008055:Lipids / Q000032:analysis*; D008099:Liver / Q000737:chemistry / Q000378:metabolism* / Q000473:pathology; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008297:Male; D010743:Phospholipids / Q000032:analysis; D012016:Reference Values
D005227:Fatty Acids; D005989:Glycerides; D008055:Lipids; D010743:Phospholipids; D006859:Hydrogen