Reversed-phase high-performance liquid chromatography of ketorolac and its application to bioequivalence studies in human serum.
A reversed-phase high-performance liquid chromatographic assay was used to study the bioequivalence of the anti-inflammatory drug (+/-)-ketorolac in human volunteers. Following deproteinization of human serum with 5% zinc sulphate solution, ketorolac was chromatographed on a 10-microns octadecylsilica column using acetonitrile-water as mobile phase and ultraviolet detection at 313 nm. Under these conditions the method was reproducible with a coefficient of variation of less than 5%. The assay procedure was linear in the range 0.25-1.5 micrograms/ml, with a sensitivity of 0.01 micrograms/ml ketorolac. The recovery of ketorolac from serum ranged from 90 to 95%.
Lupin Laboratories Limited, MIDC, Chikalthana, Aurangabad, India.
R S
RS
Chaudhary
S S
SS
Gangwal
K C
KC
Jindal
S
S
Khanna
D000700:Analgesics / Q000097:blood*; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D020910:Ketorolac; D015203:Reproducibility of Results; D014046:Tolmetin / Q000031:analogs & derivatives* / Q000097:blood
D000700:Analgesics; D000894:Anti-Inflammatory Agents, Non-Steroidal; D014046:Tolmetin; D020910:Ketorolac