Methods for measurement of antibody/antigen affinity based on ELISA and RIA.
Various enzyme-linked immunosorbent assay or radioimmunoassay methods are currently used to quantify the antibody-antigen interaction. Only those based on indirect competition--enzyme-linked immunosorbent assay or radioimmunoassay--can provide the real thermodynamic affinity of the antibody for its antigen. They can be applied to a variety of experimental situations, some of which are reviewed here.
Unité de Biochimie Cellulaire, Institut Pasteur, Paris, France.
M E
ME
Goldberg
L
L
Djavadi-Ohaniance
D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000378:metabolism; D000915:Antibody Affinity; D000937:Antigen-Antibody Reactions; D001667:Binding, Competitive; D002863:Chromogenic Compounds; D004797:Enzyme-Linked Immunosorbent Assay; D011863:Radioimmunoassay; D013816:Thermodynamics
D000911:Antibodies, Monoclonal; D002863:Chromogenic Compounds