A novel X-linked member of the human zinc finger protein gene family: isolation, mapping, and expression.
We report the partial characterization of a novel putative zinc finger gene of the Krüppel-type (ZNF81), isolated from an X Chromosome (Chr) specific library. The pattern of segregation in human-hamster somatic cell hybrids of sequences homologous to the ZNF81 finger domain has established that it resides within the Xp22.1-Xp11 region. ZNF81 represents yet another example, together with ZFX, ZNF41, and ZNF21, of members of the zinc finger gene family residing within the short arm of the human X Chr. Sequence analysis showed that ZNF81 may encode a polypeptide(s) containing tandem arrays of 12 canonical C2H2 zinc fingers of the Krüppel-type at the C-terminus. Northern analysis indicated that probes from the ZNF81 finger domain hybridize to polyadenylated transcripts present in several cell lines, a result that supports the hypothesis that it is an expressed, functional member of this multigene family.
International Institute of Genetics and Biophysics, CNR, Naples, Italy.
M
M
Marino
N
N
Archidiacono
A
A
Franzé
M
M
Rosati
M
M
Rocchi
A
A
Ballabio
G
G
Grimaldi
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D006224:Cricetinae; D004247:DNA / Q000235:genetics; D015870:Gene Expression; D015723:Gene Library; D008040:Genetic Linkage; D006801:Humans; D006822:Hybrid Cells; D051379:Mice; D008969:Molecular Sequence Data; D005810:Multigene Family; D014960:X Chromosome; D016335:Zinc Fingers / Q000235:genetics*
D004247:DNA