Evaluation of an ELISA for detection of ovine progressive pneumonia antibodies using a recombinant transmembrane envelope protein.
An enzyme-linked immunosorbent assay (ELISA) was developed using a recombinant protein corresponding to the N'-terminal hydrophilic region of transmembrane glycoprotein (TM) of ovine lentivirus. This assay reproducibly detected antibodies in sera from 207 of 212 ovine progressive pneumonia (OPP) virus-infected sheep, and the recombinant TM ELISA accurately identified 26% (35 vs. 9) more seropositive samples than did the agar gel immunodiffusion test when applied to 100 sera from an infected flock. This assay also yielded no false-positive results in 14 true negative sera. Results of these experiments were further confirmed by the recombinant TM and recombinant p25 Western blot assay. A single recombinant TM antigen, as the coating antigen in ELISA, can be used successfully for the detection of OPP virus-infected animals and can improve the sensitivity and specificity for OPP diagnosis.
Kwang
J
J
USDA, ARS, US Meat Animal Research Center, Clay Center, NE 68933.
Keen
J
J
Cutlip
R C
RC
Littledike
E T
ET
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D005069:Evaluation Studies as Topic; D005982:Glutathione Transferase; D005779:Immunodiffusion / Q000662:veterinary; D011021:Pneumonia, Progressive Interstitial, of Sheep / Q000175:diagnosis*; D011024:Pneumonia, Viral / Q000382:microbiology / Q000662:veterinary*; D011993:Recombinant Fusion Proteins; D012756:Sheep
D000914:Antibodies, Viral; D011993:Recombinant Fusion Proteins; D005982:Glutathione Transferase