Characterization of the sporulation-related gamma-D-glutamyl-(L)meso-diaminopimelic-acid-hydrolysing peptidase I of Bacillus sphaericus NCTC 9602 as a member of the metallo(zinc) carboxypeptidase A family. Modular design of the protein.
The sporulation-related gamma-D-glutamyl-(L)meso-diaminopimelic-acid-hydrolysing peptidase I of Bacillus sphaericus NCTC 9602 has been analysed by proton-induced X-ray emission. It contains 1 equivalent Zn2+ per mol of protein. As derived from gene cloning and sequencing, the B. sphaericus Zn peptidase I is a two-module protein. A 100-amino-acid-residue N-terminal domain consisting of two tandem segments of similar sequences, is fused to a 296-amino-acid-residue C-terminal catalytic domain. The catalytic domain belongs to the Zn carboxypeptidase A family, the closest match being observed with the Streptomyces griseus carboxypeptidase [Narahashi (1990) J. Biochem. 107, 879-886] and with the family prototype, bovine carboxypeptidase A. The catalytic domain of the B. sphaericus peptidase I possesses, distributed along the amino-acid sequence, peptide segments, a triad His162-Glu165-His307 and a dyad Tyr347-Glu366 that are equivalent to secondary structures, the zinc-binding triad His69-Glu72-His196 and the catalytic dyad Tyr248-Glu270 of bovine carboxypeptidase A respectively. The N-terminal repeats of the B. sphaericus peptidase I have similarity with the C-terminal repeats of the Enterococcus hirae muramidase 2, the Streptococcus (now Enterococcus) faecalis autolysin and the Bacillus phi PZA and phi 29 lysozymes, to which a role in the recognition of a particular moiety of the bacterial cell envelope has been tentatively assigned. Detergents enhance considerably the specific activity of the B. sphaericus peptidase I.
Centre d'Ingénierie des Protéines, Université de Liège, Belgium.
M L
ML
Hourdou
M
M
Guinand
M J
MJ
Vacheron
G
G
Michel
L
L
Denoroy
C
C
Duez
S
S
Englebert
B
B
Joris
G
G
Weber
J M
JM
Ghuysen
D000595:Amino Acid Sequence; D000818:Animals; D001407:Bacillus / Q000201:enzymology* / Q000502:physiology; D001483:Base Sequence; D002268:Carboxypeptidases / Q000145:classification* / Q000235:genetics / Q000378:metabolism; D043422:Carboxypeptidases A; D002417:Cattle; D003001:Cloning, Molecular; D004269:DNA, Bacterial; D003902:Detergents; D010450:Endopeptidases / Q000145:classification* / Q000235:genetics / Q000378:metabolism; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D010449:Peptide Mapping; D017386:Sequence Homology, Amino Acid; D013057:Spectrum Analysis; D013171:Spores, Bacterial; D015032:Zinc / Q000378:metabolism*
D004269:DNA, Bacterial; D003902:Detergents; D002268:Carboxypeptidases; D010450:Endopeptidases; D043422:Carboxypeptidases A; C019404:gamma-D-glutamyl-meso-diaminopimelate peptidase I; D015032:Zinc