[The immobilization of proteolytic enzymes on carbon materials].
Immobilization of proteolytic enzymes on carbon materials depends on the concentration of the protein to be immobilized, the protein/carrier ratio and pH. The kinetics of the proteolytic enzyme adsorption and combined immobilization of proteases and lytic enzymes are considered, and properties of the resultant preparations (temperature and pH optima) and the effect of gamma-sterilization are discussed. Chymotrypsin and terrilytin immobilized on carbon SKN-2P hydrolysed 0.2% solution of casein during 25-27 days with a maximum yield of 50-70%.
Sevast'ianova
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EV
Davidenko
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TI
D000327:Adsorption; D002364:Caseins / Q000378:metabolism; D002606:Charcoal; D004800:Enzymes, Immobilized / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D009391:Nephelometry and Turbidimetry; D010447:Peptide Hydrolases / Q000378:metabolism*; D013696:Temperature
D002364:Caseins; D004800:Enzymes, Immobilized; D002606:Charcoal; D010447:Peptide Hydrolases
Immobilizatsiia proteoliticheskikh fermentov na ugol'nykh materialakh.