Aluminum inhibits bone nodule formation and calcification in vitro.
Cells isolated from neonatal mouse calvariae can be induced to form mineralized nodules after exposure to ascorbic acid and beta-glycerophosphate. To determine whether aluminum inhibits nodule formation and subsequent mineralization, cells isolated from neonatal mouse calvariae were induced to form nodules and incubated with increasing concentrations of aluminum (10(-7) to 10(-5) M). Compared with control and 10(-7) M aluminum-supplemented cultures, the number of nodules formed and the number of nodules calcified were reduced in cells incubated with 10(-6) and 10(-5) M aluminum. The cumulative net calcium uptake into the nodules and their final calcium content were also decreased with 10(-6) and 10(-5) M aluminum. After 10 and 18 days of incubation, aluminum did not affect DNA synthesis or release of alkaline phosphatase but significantly inhibited collagen production. Thus aluminum induced a dose-dependent inhibition of nodule formation and calcification that may be related to its inhibition of collagen production.
Nephrology Program, University of Chicago, Illinois 60637.
S M
SM
Sprague
N S
NS
Krieger
D A
DA
Bushinsky
D000469:Alkaline Phosphatase / Q000378:metabolism; D000535:Aluminum / Q000494:pharmacology*; D000818:Animals; D001842:Bone and Bones / Q000166:cytology / Q000378:metabolism; D002113:Calcification, Physiologic / Q000187:drug effects*; D002118:Calcium / Q000493:pharmacokinetics; D002478:Cells, Cultured; D004247:DNA / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D009994:Osmolar Concentration; D010012:Osteogenesis / Q000187:drug effects*; D011506:Proteins / Q000378:metabolism
D011506:Proteins; D004247:DNA; D000535:Aluminum; D000469:Alkaline Phosphatase; D002118:Calcium