Determination of 2,2-dimethyl-N-(2,4,6-trimethoxyphenyl)-dodecanamide, a lipid regulator, in rat plasma and mesenteric lymph by reversed-phase high-performance liquid chromatography.
2,2-Dimethyl-N-(2,4,6-trimethoxyphenyl)dodecanamide (I, CI-976) has been determined in rat mesenteric lymph and plasma using a rapid and sensitive high-performance liquid chromatographic method. The samples prepared from plasma and lymph by liquid-liquid extraction were analysed on a reversed-phase C18 column isocratic conditions and ultraviolet detection. The method was applied to the determination of levels of I in Wistar rats after intraduodenal administration of 110 mg/kg of I as a lipid emulsion.
Hauss
D J
DJ
Department of Pharmaceutics, Parke-Davis Pharmaceutical Research Division, Warner Lambert Company, Morris Plains, NJ 07950.
Martin
P J
PJ
Mehta
S C
SC
Radebaugh
G W
GW
D000813:Anilides / Q000032:analysis* / Q000097:blood; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D000960:Hypolipidemic Agents / Q000032:analysis* / Q000097:blood; D008196:Lymph / Q000737:chemistry*; D008297:Male; D008643:Mesentery / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D013056:Spectrophotometry, Ultraviolet; D002785:Sterol O-Acyltransferase / Q000037:antagonists & inhibitors*
D000813:Anilides; D000960:Hypolipidemic Agents; C069304:PD 128042; D002785:Sterol O-Acyltransferase