Effects of endothelin-1 on bovine parathyroid cells.
Endothelin-1 (ET-1) is synthesized and released by parathyroid epithelium. The effects of endothelin isopeptides were studied in clonal bovine parathyroid endothelial (BPE) cells. BPE cells did not produce ET-1, but showed ETA receptors (Kd = 0.1 +/- 0.02 nM, mean +/- SE). ET-1 (10(-8)10(-11)M) increased the intracellular calcium ion concentration ([Ca2+]i) in BPE cells, while endothelin-3 (ET-3) was ineffective. The increase in [Ca2+]i was less sustained in the absence of extracellular Ca2+ ions. Moreover ET-1 induced phospholipase C (PLC) activation, as demonstrated by the increase in inositol trisphosphate. Cell growth was not affected by ET-1 in a wide range of concentrations. The present findings demonstrate: 1) BPE cells possess ETA receptors; 2) the peptide activates PLC and increases cytosolic [Ca2+]i via both a release of Ca2+ ions from intracellular calcium pool(s) and an influx of the cation from the extracellular milieu. A possible role of ET-1 as a paracrine factor in parathyroid tissue can be hypothesized.
Department of Clinical Pharmacology, University of Florence, Italy.
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A
Tanini
P
P
Failli
M
M
Maggi
F
F
Franceschelli
U
U
Frediani
L
L
Becherini
A
A
Giotti
C
C
Ruocco
M L
ML
Brandi
D000818:Animals; D002118:Calcium / Q000378:metabolism; D002417:Cattle; D002460:Cell Line; D016232:Endothelins / Q000494:pharmacology*; D004789:Enzyme Activation; D007295:Inositol Phosphates / Q000378:metabolism; D010280:Parathyroid Glands / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D017466:Receptors, Endothelin / Q000378:metabolism; D010738:Type C Phospholipases / Q000378:metabolism
D016232:Endothelins; D007295:Inositol Phosphates; D017466:Receptors, Endothelin; D010738:Type C Phospholipases; D002118:Calcium