[Role of the energy status and putrescine transport in the maintenance of the intracellular pH homeostasis in the course of alkaline and acidic shifts in Escherichia coli].
Alkaline and acidic shifts in exponentially grown Escherichia coli cultures were accompanied by an abatement of the cell energetic status (decline of ATP and energy charge and increase of the ADP and AMP pools). This event led to a suspension of the growth at (the means of) pH 9.0 and 6.0 accordingly. An increment of ornithine decarboxylase activity and an efflux of putrescine from the cells were observed in the course of alkaline shift. Environmental acidification was accompanied by intensification of the cell respiration rate and H+ATPase activity in the absence of the putrescine efflux from the cells. The involvement of the polyamine synthesizing system in the regulation of cell K+ content and the role of the primary proton pumps in the maintenance of pH homeostasis are discussed.
Tkachenko
A G
AG
Chudinov
A A
AA
Nagorskikh
T G
TG
D000136:Acid-Base Equilibrium / Q000502:physiology*; D000222:Adaptation, Physiological; D015317:Biogenic Polyamines / Q000096:biosynthesis*; D001692:Biological Transport / Q000502:physiology; D004734:Energy Metabolism / Q000502:physiology*; D004926:Escherichia coli / Q000378:metabolism*; D006706:Homeostasis / Q000502:physiology; D009955:Ornithine Decarboxylase / Q000378:metabolism; D017494:Proton Pumps / Q000502:physiology*; D011700:Putrescine / Q000378:metabolism*
D015317:Biogenic Polyamines; D017494:Proton Pumps; D009955:Ornithine Decarboxylase; D011700:Putrescine
Rol' énergeticheskogo statusa i transporta putrestsina v obespechenii gomeostaza vnutrikletochnogo pH v protsesse shchelochnykh i kislp6nykhshiftov u Escherichia coli.