Proteins isolated from the venom of the common tiger snake (Notechis scutatus scutatus) promote hypotension and hemorrhage.
Notechis scutatus scutatus venom contains several toxic acidic proteins called HTa-i which promote hypotension and hemorrhage in mice. They have apparent mol. wts in the 18,000-21,000 range, i.v. LD50 values between 0.5 and 1.5 micrograms/g, and no detectable phospholipase, arginine esterase, proteolytic or hemolytic activities. A polyclonal antibody raised against HTg binds to other purified proteins, suggesting that they are isoforms of the same protein. Many other elapid crude venoms contain proteins which recognize the polyclonal antibody raised against HTg. Crotalid and viperid crude venoms do not recognize this antibody, although some of their component proteins are known to exhibit hypotensive and hemorrhagic activities. A combination of gel-filtration on Sephacryl S-200, cation-exchange and anion-exchange chromatography allows isolation of the N. s. scutatus proteins in high purity. They are the first hypotension-inducing proteins to be purified from an Australian elapid.
Francis
B
B
Department of Molecular Biology, University of Wyoming, Laramie 82071.
Williams
E S
ES
Seebart
C
C
Kaiser
I I
II
D000818:Animals; D000918:Antibody Specificity; D001794:Blood Pressure / Q000187:drug effects*; D015153:Blotting, Western; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003435:Crotalid Venoms / Q000276:immunology; D003864:Depression, Chemical; D004546:Elapid Venoms / Q000032:analysis / Q000276:immunology / Q000633:toxicity*; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006470:Hemorrhage / Q000139:chemically induced* / Q000473:pathology; D007274:Injections, Intraperitoneal; D007275:Injections, Intravenous; D051379:Mice; D011506:Proteins / Q000302:isolation & purification / Q000633:toxicity*; D014757:Viper Venoms / Q000276:immunology
D003435:Crotalid Venoms; D004546:Elapid Venoms; D011506:Proteins; D014757:Viper Venoms