An efficient 1-tube PCR method for internal site-directed mutagenesis of large amplified molecules.
Marini
F
F
Department of Molecular Pathology, University of Texas M.D. Anderson Cancer Center, Houston 77030.
Naeem
A
A
Lapeyre
J N
JN
D001483:Base Sequence; D004277:DNA, Single-Stranded; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D016133:Polymerase Chain Reaction / Q000379:methods*
D004277:DNA, Single-Stranded