A method for sampling representative muscular venous blood during exercise in rats.
A technique for chronic cannulation of the muscular branch of the femoral vein in the rat is described. The method was validated by the application of vascular corrosion casts and comparative analysis of lactate concentration with mixed venous blood and arterial samples taken through the cannulas during lower hindlimb muscle contraction in anaesthetized rats.
Departamento de Bioquímica y Fisiología, Universidad de Barcelona, Spain.
T
T
Pagés
J A
JA
Fernández
C
C
Adán
A
A
Gámez
G
G
Viscor
L
L
Palacios
D000818:Animals; D000830:Animals, Laboratory; D001800:Blood Specimen Collection / Q000662:veterinary*; D002404:Catheterization / Q000662:veterinary*; D005268:Femoral Vein; D007773:Lactates / Q000097:blood; D008297:Male; D010805:Physical Conditioning, Animal; D051381:Rats; D017208:Rats, Wistar
D007773:Lactates