Surface plasmon resonance for detection and measurement of antibody-antigen affinity and kinetics.
Kinetic and affinity information on biospecific interaction can be generated by analysis of adsorption of analyte to immobilized ligand on a sensor based analytical system. The analysis can be performed free of label and directly using culture supernatants. It was found to be particularly valuable for analysis of antibody-antigen interactions.
Malmqvist
M
M
Pharmacia Biosensor AB, Uppsala, Sweden.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000915:Antibody Affinity; D015374:Biosensing Techniques; D015686:Gene Products, env / Q000032:analysis / Q000276:immunology; D015483:HIV Antibodies / Q000276:immunology; D016655:HIV Core Protein p24 / Q000032:analysis / Q000276:immunology; D019302:HIV Envelope Protein gp160; D006801:Humans; D007163:Immunosorbent Techniques / Q000295:instrumentation; D007700:Kinetics; D008024:Ligands; D051379:Mice; D015202:Protein Engineering; D011498:Protein Precursors / Q000032:analysis / Q000276:immunology; D011994:Recombinant Proteins / Q000276:immunology; D013499:Surface Properties
D000911:Antibodies, Monoclonal; D015686:Gene Products, env; D015483:HIV Antibodies; D016655:HIV Core Protein p24; D019302:HIV Envelope Protein gp160; D008024:Ligands; D011498:Protein Precursors; D011994:Recombinant Proteins