Preservation of small bowel grafts--a comparison with two standard solutions.
This study investigates the use of EuroCollins (EC) and University of Wisconsin (UW) solution, two major preservation fluids, for small bowel preservation. After in situ flushing, grafts were cold-stored at 4 degrees C with either EC for 30 min (group 1a), 6 h (group 1b) and 12 h (group 1c) or with UW for 30 min (group 2a), 6 h (group 2b) and 12 h (group 2c). Using UW, cold ischemia was extended to up to 18 h (group 2d). As a control, small intestines were flushed and stored for the same time periods in cold saline (group 3a-c). Survival in group 1b was 66% versus 100% in group 2b. After 12 h 80% survived in group 2c, but there were not survivors in group 1c. After 18 h of cold storage, survival was only observed in group 2d (25%). Saline was ineffective after 6 h of preservation. Histology at the end of preservation revealed characteristic changes for EC (intracellular vacuoles) and UW (amorphic granules). We conclude that with UW small bowel can be preserved for up to 12 h.
Department of Transplant Surgery University Hospital, Innsbruck, Austria.
W
W
Thaler
G
G
Oberhuber
G
G
Klima
R
R
Margreiter
T
T
Schmid
D000241:Adenosine; D000493:Allopurinol; D000818:Animals; D003080:Cold Temperature; D005069:Evaluation Studies as Topic; D005978:Glutathione; D006085:Graft Survival; D006982:Hypertonic Solutions / Q000494:pharmacology; D007328:Insulin; D007408:Intestinal Absorption; D007421:Intestine, Small / Q000378:metabolism / Q000473:pathology / Q000637:transplantation*; D008297:Male; D008320:Maltose / Q000493:pharmacokinetics; D019188:Organ Preservation Solutions; D011887:Raffinose; D051381:Rats; D011917:Rats, Inbred Lew; D015427:Reperfusion Injury / Q000473:pathology; D012996:Solutions; D014021:Tissue Preservation / Q000379:methods*
C037096:Euro-Collins' solution; D006982:Hypertonic Solutions; D007328:Insulin; D019188:Organ Preservation Solutions; D012996:Solutions; C055206:University of Wisconsin-lactobionate solution; D000493:Allopurinol; D008320:Maltose; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose