Cytogenetic analysis of uncleaved oocytes after intracytoplasmic sperm injection.
OBJECTIVE
This work analyzes the causes of cleavage failure after intracytoplasmic sperm injection (ICSI) and the effect of the procedure on the chromosomes of the oocytes.


METHODS
Ninety-seven uncleaved oocytes from 39 patients with severe male infertility or repeated IVF failure were fixed; 79 were analyzable. We checked the decondensation stage of spermatozoa nucleus and the chromosomal abnormalities of the oocytes.


RESULTS
Among the fixed oocytes, the spermatozoa nucleus was present in 97% of the cases, and it was undecondensed in 89% of the cases, showing no evolution at all. A low rate (2.6%) of premature chromosome condensation (PCC) of the spermatozoa and a low rate (2.5%) of female diploïdy were observed. Among the oocytes that could be karyotyped, we observed a high rate (45%) of chromosome breakage.


CONCLUSIONS
ICSI fertilization failure was due mostly to the complete lack of evolution of the spermatozoa nucleus. Oocyte selection before ICSI seemed to lower the PCC rate. The high rate of oocyte chromosomal breakage rate has to be confirmed.
Gyneco-obstetric Department, Hôpital Antoine Béclère, Clamart, France.
M
M
Bergère
J
J
Selva
M
M
Volante
M
M
Dumont
A
A
Hazout
F
F
Olivennes
R
R
Frydman
D002869:Chromosome Aberrations / Q000235:genetics*; D025063:Chromosome Disorders; D005260:Female; D006801:Humans; D015994:Incidence; D007315:Insemination, Artificial; D007621:Karyotyping; D008297:Male; D009865:Oocytes / Q000648:ultrastructure; D013094:Spermatozoa / Q000648:ultrastructure