Serological survey of canine distemper virus infection using enzyme-linked immunosorbent assay.
For detection of antibodies against canine distemper virus (CDV), an enzyme-linked immunosorbent assay (ELISA) was developed using a crude extract from cells infected with the Onderstepoort strain of CDV as antigen. Twenty-six sera from dogs experimentally vaccinated with the Snyder-Hill strain of CDV were compared by ELISA and a standard virus neutralization (NV) test. Since a good correlation between the titers obtained by both tests was observed, ELISA was considered to be a rapid and reliable method for a serological survey of CDV infection. When a total of 167 sera from dogs suspected of canine distemper under natural conditions were examined by the ELISA, 29 of the sera (17%) were found to have low VN antibody titers and high ELISA titers. The reason for the discrepancy in the titers was discussed.
Department of Veterinary Microbiology, Faculty of Agriculture, University of Tokyo, Japan.
T
T
Gemma
N
N
Miyashita
Y S
YS
Shin
M
M
Okita
T
T
Mori
K
K
Iwatsuki
T
T
Mikami
C
C
Kai
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D002522:Chlorocebus aethiops; D004216:Distemper / Q000097:blood / Q000276:immunology* / Q000503:physiopathology; D004217:Distemper Virus, Canine / Q000276:immunology*; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary; D012044:Regression Analysis; D012680:Sensitivity and Specificity; D014709:Vero Cells
D000914:Antibodies, Viral