Porphyromonas-like gram-negative rods in naturally occurring periodontitis in dogs.
A total of 259 Gram-negative Porphyromonas-like rods isolated from subgingival plaque samples of 16 family-owned dogs with naturally occurring periodontitis were characterized phenotypically by biochemical reactions, metabolic end products and enzymatic activities (API-ZYMTM, RoscoTM). Four distinct groups were found. Group A isolates (63) were asaccharolytic, lipase negative, trypsin positive and produced phenylacetic acid (PAA) from peptone-yeast extract glucose broth. Unlike P. gingivalis strains they were catalase positive. Group B isolates (42) differed from those of group A by a positive lipase reaction and from those of group D by failing to ferment sugars. Group C isolates (88) were asaccharolytic and did not produce PAA. They were alpha-fucosidase, N-acetyl-beta-glucosaminidase (beta-NAG) and trypsin negative, resembling P. endodontalis, but unlike human isolates, they were catalase positive. Subgroup C.1 isolates (6) differed from those of parent group C by producing minor amounts of PAA, and subgroup C.2 isolates (12) were beta-NAG positive. Group D isolates (46) were weakly fermentative, lipase, catalase and trypsin positive, and produced PAA. They resembled the B (P.) salivosus type strain which, in our hands, fermented weakly glucose, lactose and mannose. Two isolates could not be assigned to any of the previous groups.
National Public Health Institute, Anaerobe Reference Laboratory, Helsinki, Finland.
J
J
Karjalainen
A
A
Kanervo
M L
ML
Väisänen
B
B
Forsblom
E
E
Sarkiala
H
H
Jousimies-Somer
D000818:Animals; D001426:Bacterial Proteins / Q000032:analysis; D015373:Bacterial Typing Techniques; D001438:Bacteroidaceae / Q000145:classification / Q000302:isolation & purification* / Q000378:metabolism; D050260:Carbohydrate Metabolism; D002374:Catalase / Q000032:analysis; D003773:Dental Plaque / Q000382:microbiology; D004283:Dog Diseases / Q000382:microbiology*; D004285:Dogs / Q000382:microbiology; D010518:Periodontitis / Q000382:microbiology / Q000662:veterinary*; D010641:Phenotype; D010648:Phenylacetates / Q000378:metabolism; D013045:Species Specificity
D001426:Bacterial Proteins; D010648:Phenylacetates; D002374:Catalase; C025136:phenylacetic acid