Inhibition of central angiotensin responses by angiotensin type-1 receptor antibody.
Angiotensin type-1 receptor subtypes (AT1) are implicated in the physiological actions of angiotensin II in the brain. In the present study we used an AT1 receptor antibody and a polymerase chain reaction--synthesized AT1 receptor complementary DNA to show that the hypothalamus expresses significantly higher levels of AT1 receptor messenger RNA and protein compared with the brain stem. Intracerebroventricular injections of AT1-specific antibody blocks the dipsogenic and blood pressure responses induced by centrally injected angiotensin II. These results demonstrate the expression of AT1 receptor gene in the brain and that the AT1 receptor antibody is able to inhibit the physiological responses of angiotensin II mediated by the brain.
Department of Physiology, College of Medicine, University of Florida, Gainesville.
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EM
Richards
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D
Lu
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B
Zelezna
M I
MI
Phillips
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M
Trolliet
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Sumners
M K
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Raizada
D000804:Angiotensin II / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D057911:Angiotensin Receptor Antagonists; D000818:Animals; D000906:Antibodies / Q000276:immunology*; D001794:Blood Pressure / Q000502:physiology; D001921:Brain / Q000378:metabolism*; D004326:Drinking / Q000502:physiology; D007276:Injections, Intraventricular; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011921:Rats, Inbred WKY; D017207:Rats, Sprague-Dawley; D011945:Receptors, Angiotensin / Q000235:genetics / Q000276:immunology* / Q000378:metabolism
D057911:Angiotensin Receptor Antagonists; D000906:Antibodies; D012333:RNA, Messenger; D011945:Receptors, Angiotensin; D000804:Angiotensin II