Determination of enantiomers of bupivacaine in serum using an on-line coupled three column liquid chromatographic system.
An on-line coupled HPLC system is described for the determination of the enantiomers of bupivacaine in serum. The method involves three steps: (i) pre-concentration and clean-up; (ii) the determination of the racemates on a reversed-phase column; and (iii) the separation of the racemates, heart-cut from the reversed-phase column, on an alpha-glycoprotein column. The method is suitable to determine the enantiomers in serum down to 0.1 micrograms ml-1 and can be fully automated. The bupivacaine time course of patients will be shown.
Laboratory for Analytical Chemistry, University of Amsterdam, The Netherlands.
J J
JJ
Butter
J C
JC
Kraak
H
H
Poppe
D002045:Bupivacaine / Q000097:blood* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D013237:Stereoisomerism
D002045:Bupivacaine