The in vivo antibody response in rat gut-associated lymphoid tissue (GALT) after immunization with bacterial polysaccharide antigen.
Gut mucosal immune responses to bacterial polysaccharide antigen in rats were investigated in vivo. Rats were immunized with pneumococcal polysaccharide type 3 (PPS-3) via different routes, i.e. in the Peyer's patch (iPP), in the colon (ic), in the peritoneal cavity (ip), and intravenously (iv). The development of specific antibody-forming cells (AFC) and their isotypes in the intestinal mucosa, gut-associated lymphoid tissue (GALT), mesenteric lymph nodes (MLN) and spleen were studied by immunohistochemistry. Furthermore, the serum antibody levels were measured by enzyme-linked immunosorbent assay (ELISA). The results showed that iPP immunization evoked high numbers of anti-PPS-3 AFC of the IgA isotype in the mucosa of the small intestine and in the PP. On the contrary, the ic route did not elicit a mucosal response, though a few AFC were found in the MLN and spleen. Following ip priming, a specific IgA response was found, especially in MLN and spleen, and a low response was detected in the villi. A high response was found in the parathymic lymph nodes (PTLN). Iv immunization gave rise to the development of AFC in the spleen, particularly of the IgM isotype. We failed to induce mucosal responses to PPS-3 antigen in the colon, irrespective of the route of immunization.
Department of Cell Biology, Medical Faculty Vrije Universiteit, Amsterdam.
M
M
Soesatyo
G P
GP
Van den Dobbelsteen
E P
EP
Van Rees
J
J
Biewenga
T
T
Sminia
D000818:Animals; D000942:Antigens, Bacterial / Q000096:biosynthesis*; D007132:Immunoglobulin Isotypes / Q000276:immunology; D007136:Immunoglobulins / Q000276:immunology; D007413:Intestinal Mucosa / Q000276:immunology*; D008221:Lymphoid Tissue / Q000276:immunology*; D008297:Male; D011135:Polysaccharides, Bacterial / Q000276:immunology*; D051381:Rats; D017208:Rats, Wistar; D013296:Streptococcus pneumoniae / Q000276:immunology*
D000942:Antigens, Bacterial; D007132:Immunoglobulin Isotypes; D007136:Immunoglobulins; D011135:Polysaccharides, Bacterial; C047620:pneumococcal polysaccharide, type II