[Preparation of a non-defective adenovirus vector based on the Ad5 genome with the E3 region replaced with plasmid DNA with a kanamycin- resistance gene].
The plasmid pH5KV14 containing the right end of the Ad5 viral genome (45-100 map units) with deleted nonessential E3 region (78.5-84 map units) has been constructed. A helper-independent recombinant virus Ad5KV14 has been obtained as a result of in vivo transformation of the line 293 human cells by the pH5KV14 plasmid DNA and an EcoRI-A DNA fragment of the virus Ad5 and subsequent recombination. A nondefective recombinant virus Ad5KV17 containing the Ad5 genome with the E3 region substituted for the plasmid DNA fragment coding for the kanamycin-resistance has been constructed by a similar procedure.
K K
KK
Doronin
V A
VA
Krugliak
N F
NF
Grinenko
E V
EV
Progonova
N A
NA
Grodnitskaia
B S
BS
Naroditskiĭ
T I
TI
Tikhonenko
D000256:Adenoviridae / Q000235:genetics*; D002478:Cells, Cultured; D004274:DNA, Recombinant / Q000235:genetics*; D005822:Genetic Vectors; D016679:Genome, Viral; D006801:Humans; D007613:Kanamycin Resistance / Q000235:genetics*; D010957:Plasmids; D017384:Sequence Deletion
D004274:DNA, Recombinant
Poluchenie nedefektnogo adenovirusnogo vektora na osnove genoma Ad5 s E3-oblast'iu, zameshchennoĭ plazmidnoĭ DNK s genom ustoĭchivosti k kanamitsinu.