West Nile virus infection induces susceptibility of in vitro outgrown murine blastocysts to specific lysis by paternally directed allo-immune and virus-immune cytotoxic T cells.
Day 3 post-coitum BALB/c and (BALB/c x CBA/H)F1 blastocysts were isolated and hatched in replicate wells. Some were treated with interferon-gamma (IFN-gamma). Whilst others were infected with West Nile Virus (WNV) at 100 plaque-forming units per cell, for 18 h. Controls were mock-treated. Gamma-irradiated (2000 rads) CBA/H, (paternal) WNV-specific and allo(CBA/H)-specific cytotoxic T (Tc) cells were then added to replicates of infected, mock-infected or IFN-gamma-treated cultures for 20 h. [3H]Thymidine was then added for a further 8 h. [3H]Thymidine incorporation was inhibited by 40-50% in WNV-infected cultures exposed to WNV-paternal-specific Tc cells and by 30-40% in WNV-infected cultures exposed to allo-paternal-specific Tc cells compared to similarly exposed, uninfected, or unexposed, WNV-infected, or unexposed, uninfected cultures. No significant differences in [3H]thymidine incorporation were found between these controls and IFN-gamma-treated cultures exposed to allo-paternal-specific Tc cells or IFN-gamma-treated cultures not exposed to Tc cells. Parallel exposure of L929 fibroblasts to the same Tc cells irradiated with 500-8000 rads in doubling doses, showed that irradiation did not alter the efficacy or specificity of the Tc cells. Relevance to maternal anti-viral immune responses during implantation is discussed.
Department of Pathology, University of Sydney, N.S.W., Australia.
N J
NJ
King
A
A
Mullbacher
L
L
Tian
J C
JC
Rodger
B
B
Lidbury
R T
RT
Hla
D000818:Animals; D000956:Antigens, Viral / Q000276:immunology; D001755:Blastocyst / Q000276:immunology* / Q000382:microbiology; D002460:Cell Line; D002478:Cells, Cultured; D003601:Cytotoxicity Tests, Immunologic; D003602:Cytotoxicity, Immunologic / Q000187:drug effects / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D005260:Female; D005347:Fibroblasts / Q000276:immunology; D005720:Gamma Rays; D015395:Histocompatibility Antigens Class I / Q000276:immunology; D007111:Immunity, Cellular / Q000528:radiation effects; D007371:Interferon-gamma / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008808:Mice, Inbred CBA; D011247:Pregnancy; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology* / Q000528:radiation effects; D014901:West Nile Fever / Q000276:immunology; D014902:West Nile virus / Q000276:immunology*
D000956:Antigens, Viral; D015395:Histocompatibility Antigens Class I; D007371:Interferon-gamma