[Detection of Chlamydia trachomatis infection by PCR in first voided urines in men].
The goal of this study was to evaluate whether the new commercially available PCR-based assay Amplicor C. trachomatis (Roche Molecular Systems) could improve the diagnosis of chlamydial urogenital infections in men, compared with cell culture of C. trachomatis considered as the reference method. A total of 466 men attending the STD clinic were tested by the Amplicor test in urine and by cell culture in urethra. The prevalence of C. trachomatis was 13.7% (64/466) by cell culture and 14.4% (67/466) by the Amplicor test. After resolution of the discrepant results, the sensitivity of culture was 91.4% in male urethral specimens. The resolved sensitivity of the PCR assay was 92.7% in male urine and the specificity was 99.5%. We concluded that this rapid PCR-based assay showed an improvement in quality for diagnosing C. trachomatis infections in men.
Laboratoire de bactériologie virologie hyiène, hôpital Saint-Louis, Paris, France.
A
A
Bianchi
N
N
Brunat
S
S
Henry
M
M
Janier
F
F
Lassau
P
P
Morel
C
C
Scieux
D000293:Adolescent; D000328:Adult; D000368:Aged; D002690:Chlamydia Infections / Q000382:microbiology / Q000652:urine*; D002692:Chlamydia trachomatis; D006801:Humans; D008297:Male; D052801:Male Urogenital Diseases / Q000382:microbiology / Q000652:urine*; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods*; D015995:Prevalence; D012680:Sensitivity and Specificity; D013048:Specimen Handling
Détection d'une infection masculine à Chlamydia trachomatis par PCR sur premier jet d'urine.