The 3' untranslated region of satellite tobacco necrosis virus RNA stimulates translation in vitro.
The RNA of satellite tobacco necrosis virus (STNV) is a monocistronic messenger that lacks both a 5' cap structure and a 3' poly(A) tail. We show that in a cell-free translation system derived from wheat germ, STNV RNA lacking the 600-nucleotide trailer is translated an order of magnitude less efficiently than full-size RNA. Deletion analyses positioned the translational enhancer domain (TED) within a conserved hairpin structure immediately downstream from the coat protein cistron. TED enhances translation when fused to a heterologous mRNA, but the level of enhancement depends on the nature of the 5' untranslated sequence and is maximal in combination with the STNV leader. The STNV leader and TED have two regions of complementarity. One of the complementary regions in TED resembles picornavirus box A, which is involved in cap-independent translation but which is located upstream of the coding region.
Danthinne
X
X
Plant Genetic Systems N.V., Ghent, Belgium.
Seurinck
J
J
Meulewaeter
F
F
Van Montagu
M
M
Cornelissen
M
M
D001483:Base Sequence; D002213:Capsid / Q000096:biosynthesis / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D005814:Genes, Viral; D007700:Kinetics; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides; D010942:Plant Viruses / Q000235:genetics*; D010947:Plants, Toxic; D014176:Protein Biosynthesis; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D015183:Restriction Mapping; D017384:Sequence Deletion; D014026:Nicotiana / Q000382:microbiology; D014158:Transcription, Genetic; D015678:Viral Structural Proteins / Q000235:genetics
D004279:DNA, Viral; D009838:Oligodeoxyribonucleotides; D012367:RNA, Viral; D015678:Viral Structural Proteins