Direct sequencing of double-stranded PCR products incorporating a chemiluminescent detection procedure.
A simple and reliable method is described for direct sequencing of material generated by the polymerase chain reaction (PCR). Sequencing reactions can be performed directly on PCR products without the need for purification of the template by removal of residual deoxyribonucleoside triphosphates or primers. The coupling of a chemiluminescent detection system with the use of the same primers in the initial and sequencing PCR's allows for sequencing of a number of PCR products on the one gel.
Victorian Institute of Forensic Pathology, Monash University, South Melbourne, Australia.
A M
AM
Douglas
A M
AM
Georgalis
B A
BA
Atchison
D001709:Biotechnology; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D008163:Luminescent Measurements; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA / Q000379:methods*
D004247:DNA