Differential expression of pyrimidine dimer-binding proteins in normal and UV light-treated vertebrate cells.
The expression of UV damage-specific DNA-binding proteins was examined in various phylogenetically distant species with differing DNA repair phenotypes. Two distinct constitutive DNA-binding activities, one specific for cyclobutane pyrimidine dimers and the other for non-cyclobutane dimer photoproducts, were detected. The expression of these binding activities was found to be variable throughout the animal kingdom: cold-blooded vertebrates show a constitutive cyclobutane dimer-binding activity exclusively, and primates reveal only non-cyclobutane binding activity. In contrast, birds and marsupials appear to express both types of binding activities. The kinetics of expression (rather than the constitutive presence) of these UV damage-specific DNA-binding activities after UV treatment correlate with the cell's capacity for DNA repair. In addition, cyclobutane pyrimidine dimer-binding activities could be detected only in cells with established photoreactivating activity.
McLenigan
M
M
Section on Viruses and Cellular Biology, National Institute of Child Health and Human Development, Bethesda, MD 20892.
Levine
A S
AS
Protić
M
M
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D004249:DNA Damage; D004260:DNA Repair / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D010641:Phenotype; D011740:Pyrimidine Dimers / Q000378:metabolism*; D013045:Species Specificity; D014466:Ultraviolet Rays; D014714:Vertebrates
D004268:DNA-Binding Proteins; D009838:Oligodeoxyribonucleotides; D011740:Pyrimidine Dimers