Levels of androgens and danazol metabolites in serum during danazol therapy.
Thirteen women who had endometriosis were treated with danazol (300 to 400 mg/d). Levels of androgens and danazol metabolites in serum and the influences of danazol metabolites on the assays for serum androgen were investigated during danazol therapy. Serum DHEAS significantly increased (P < 0.05), but serum DHEA slightly decreased. Serum T levels, measured by direct assay, were markedly elevated. However, measured after HPLC separation, the T levels in serum were significantly decreased (P < 0.05). There were considerable cross-reactions between danazol metabolites and androgens (T, DHEA, and A) in RIA. Purification of androgens using column chromatography was necessary to measure serum androgens precisely.
Murakami
K
K
Department of Obstetrics and Gynecology, Kanazawa University School of Medicine, Japan.
Nakagawa
T
T
Yamashiro
G
G
Araki
K
K
Akasofu
K
K
D000328:Adult; D000728:Androgens / Q000097:blood*; D003613:Danazol / Q000097:blood* / Q000627:therapeutic use; D003687:Dehydroepiandrosterone / Q000097:blood; D004715:Endometriosis / Q000097:blood* / Q000188:drug therapy; D005260:Female; D006801:Humans; D008875:Middle Aged; D013739:Testosterone / Q000097:blood
D000728:Androgens; D013739:Testosterone; D003687:Dehydroepiandrosterone; D003613:Danazol