Erythropoietin receptor binds to Friend virus gp55 through other membrane components.
Direct interactions of Friend spleen focus-forming virus glycoprotein gp55 with either erythropoietin receptor (EpoR) or interleukin (IL)2 receptor beta chain (IL2R) (but not with IL3 receptor) have been reported to induce factor-independent prolonged proliferation of erythroid or lymphoid cells. In order to clarify the molecular mechanism by which EpoR-gp55 complex transmits an aberrant growth signal in the absence of erythropoietin, various chimeric receptors constituted with IL2R, EpoR or IL3 receptor were constructed. It was found that coexpression of gp55 and the chimeric receptors containing the cytoplasmic domains of EpoR and the extracellular domains of IL3 (or IL2) receptor in IL3-dependent Ba/F3 cells results in factor-independent growth. Since gp55 in cell membrane has only a two amino acid tail in the cytoplasmic domains and thus cannot interact with EpoR in cytoplasm, our data suggest that gp55 does not bind EpoR directly but interacts with EpoR through third membrane component(s).
Tsukuba Life Science Center, Institute of Physical and Chemical Research (RIKEN), Ibaraki, Japan.
A
A
Kishi
T
T
Chiba
M
M
Sugiyama
M
M
Machide
Y
Y
Nagata
H
H
Amanuma
H
H
Taira
T
T
Katsumata
K
K
Todokoro
D000818:Animals; D001402:B-Lymphocytes; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D004921:Erythropoietin / Q000494:pharmacology*; D005622:Friend murine leukemia virus / Q000378:metabolism*; D015686:Gene Products, env / Q000378:metabolism*; D005822:Genetic Vectors; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology; D007700:Kinetics; D051379:Mice; D010957:Plasmids; D017467:Receptors, Erythropoietin / Q000235:genetics / Q000378:metabolism*; D015375:Receptors, Interleukin-2 / Q000235:genetics / Q000378:metabolism; D016185:Receptors, Interleukin-3 / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D013155:Spleen Focus-Forming Viruses / Q000378:metabolism*; D014162:Transfection; D014759:Viral Envelope Proteins / Q000378:metabolism*
D015686:Gene Products, env; D007376:Interleukin-2; D017467:Receptors, Erythropoietin; D015375:Receptors, Interleukin-2; D016185:Receptors, Interleukin-3; D011993:Recombinant Fusion Proteins; D014759:Viral Envelope Proteins; C078780:glycoprotein gp55, Friend spleen focus-forming virus; D004921:Erythropoietin