Distribution of calcium binding protein mRNAs in rat cerebellar cortex.
The distribution of three calcium binding protein mRNAs in the rat cerebellar cortex was investigated using alkaline phosphatase labelled specific antisense oligodeoxynucleotide probes. Calbindin D28k mRNA was detected in the Purkinje cells, parvalbumin mRNA was located in the Purkinje cells and also in basket/stellate cells of the molecular layer. Calretinin in contrast was found only in the granule cell layer. Use of multiple alkaline phosphatase (AP)-labelled oligodeoxynucleotides resulted in an increase in signal strength and reduced detection time with no increase in background staining indicating the utility of these enzyme labelled probes for non-isotopic in situ.
Department of Neurobiology AFRC, Institute of Animal Physiology and Genetics Research, Babraham, Cambridge, UK.
K
K
Kadowaki
E
E
McGowan
G
G
Mock
S
S
Chandler
P C
PC
Emson
D000818:Animals; D064092:Calbindin 1; D064032:Calbindin 2; D064026:Calbindins; D002525:Cerebellar Cortex / Q000166:cytology / Q000378:metabolism*; D017403:In Situ Hybridization; D008297:Male; D015345:Oligonucleotide Probes; D011689:Purkinje Cells / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D064030:S100 Calcium Binding Protein G / Q000235:genetics*; D014018:Tissue Distribution
C576500:Calb1 protein, rat; C576512:Calb2 protein, rat; D064092:Calbindin 1; D064032:Calbindin 2; D064026:Calbindins; D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D064030:S100 Calcium Binding Protein G