Biosynthesis of corticotropin-releasing hormone in human T-lymphocytes.
Corticotropin-releasing hormone (CRH) is a 41-amino acid neuropeptide which increases the transcription of the proopiomelanocortin (POMC) gene, as well as the biosynthesis and secretion of POMC-derived peptides. Using a specific human CRH radioimmunoassay we have shown that human T-lymphocytes contain immunoreactive CRH. We studied the effects of phytohemagglutinin (PHA) and 12-O-tetradecanoylphorbol-13-acetate (TPA) on the biosynthesis of CRH in human T-lymphocyte cell cultures. A significant increase in CRH mRNA levels was observed in human lymphocytes after 12 h of PHA/TPA treatment, while the levels decreased after 22 h. These findings could imply an immunomodulatory role for CRH that could be due to autocrine and/or paracrine interactions.
Department of Psychiatry and Neurochemistry, University of Lund, Sweden.
R
R
Ekman
B
B
Servenius
M G
MG
Castro
P J
PJ
Lowry
A S
AS
Cederlund
O
O
Bergman
H O
HO
Sjögren
D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D003346:Corticotropin-Releasing Hormone / Q000096:biosynthesis*; D006801:Humans; D009693:Nucleic Acid Hybridization; D010835:Phytohemagglutinins / Q000494:pharmacology; D011863:Radioimmunoassay; D013601:T-Lymphocytes / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D010835:Phytohemagglutinins; D003346:Corticotropin-Releasing Hormone; D013755:Tetradecanoylphorbol Acetate