Tissue renin: focus on vascular angiotensin formation.
Local vascular formation of angiotensin (Ang) peptides was investigated in isolated, plasma-free perfused rat hindquarters. Analysis of perfusate by high performance liquid chromatography and radioimmunoassay demonstrated spontaneous releases of Ang I and II from hindlimb vasculature. Captopril suppressed Ang II and increased Ang I levels. Formation of Ang peptides was abolished by bilateral nephrectomy but unaltered by subtotal 5/6 nephrectomy. Infused renin was taken up by hindlimb vasculature and led to substantial increases of local Ang formation and perfusion pressure. Both effects were sensitive to captopril (CAS 62571-86-2) and to the renin inhibitor H-142 (H-Pro-His-Pro-Phe-His-Leu-Val-Ile-His-OH). Conversion of Ang I to Ang II in hindquarter vasculature was approximately 75% and completely suppressed by captopril. Evidence for and against local vascular synthesis of renin is briefly discussed. In conclusion, the data indicate substantial local vascular formation of Ang I and II which, however, appears to be mainly due to plasma-derived renin.
Hilgers
K F
KF
Department of Medicine-Nephrology, University of Erlangen-Nuremberg, Fed. Rep. of Germany.
Mann
J F
JF
D000595:Amino Acid Sequence; D000803:Angiotensin I / Q000378:metabolism; D000804:Angiotensin II / Q000096:biosynthesis*; D000818:Animals; D001808:Blood Vessels / Q000378:metabolism*; D008297:Male; D008969:Molecular Sequence Data; D051381:Rats; D017207:Rats, Sprague-Dawley; D012083:Renin / Q000378:metabolism* / Q000494:pharmacology
D000804:Angiotensin II; D000803:Angiotensin I; D012083:Renin