Evaluation of the Vitek Systems Gram-Positive Identification card for species identification of coagulase-negative staphylococci.
Vitek Systems' Gram-Positive Identification test (GPI) card was evaluated for the ability to identify 12 coagulase-negative Staphylococcus species and subspecies. The bionumber generated from the GPI card was examined for its potential use in epidemiological studies. Results indicated that the GPI card had a high degree of correlation with the conventional methods of identification. The species identified with the greatest accuracy were Staphylococcus epidermidis (92%), S. haemolyticus (95%), S. capitis subsp. capitis (88%), and S. saprophyticus (100%). S. hominis (63%) was identified with the least accuracy. The bionumber was found to have limited epidemiological value because of the frequent occurrence of a few major bionumbers.
Department of Microbiology, North Carolina State University, Raleigh 27695-7614.
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Bannerman
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Kleeman
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Kloos
D015373:Bacterial Typing Techniques / Q000706:statistics & numerical data; D003030:Coagulase / Q000378:metabolism; D005069:Evaluation Studies as Topic; D006801:Humans; D012680:Sensitivity and Specificity; D013045:Species Specificity; D013210:Staphylococcus / Q000145:classification* / Q000201:enzymology / Q000302:isolation & purification
D003030:Coagulase