Influence of lactobacilli on the adhesion of Staphylococcus aureus and Candida albicans to fibers and epithelial cells.
The ability of organisms to adhere to and form biofilms on fibrous materials is believed to be an important initiating step in the induction of several diseases, such as toxic shock syndrome. Using an in vitro assay, a moderately hydrophobic strain of Staphylococcus aureus (water contact angle 35 degrees) and a hydrophilic Candida albicans (shown by a hexadecane test) were highly adherent to commercial diaper fibers. The lumen side of the diaper was porous and the fibers were very hydrophobic (> 140 degrees), but the internal section was very hydrophilic (0 degrees), presumably for lus strains was present. Surfaces precoated with lactobacilli inhibited staphylococcal adhesion by 26-97%, and candida by 0-67%. When the lactobacilli were used to challenge adherent pathogens, there was 99% displacement of the S. aureus and up to 91% displacement of C. albicans. Hydrophobic L. acidophilus 76 (54 degrees) and T-13 (80 degrees) were the most effective of five Lactobacillus isolates tested at interference by precoating. The moderately hydrophilic L. casei var rhamnosus GR-1 (33 degrees) was the most effective at displacing the yeast. Experiments with uroepithelial cells also showed that the lactobacilli could significantly interfere with the adhesion of both pathogens to the cells. The results demonstrate the rapidity with which two pathogens adhered to fibers and epithelial cells, and raised the possibility that members of the normal female urogenital flora might interfere with infections caused by these organisms.
Department of Microbiology and Immunology, University of Western Ontario, London, Canada.
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D000328:Adult; D001422:Bacterial Adhesion / Q000502:physiology*; D018441:Biofilms / Q000254:growth & development*; D002176:Candida albicans / Q000254:growth & development*; D002448:Cell Adhesion / Q000502:physiology; D003963:Diaper Rash / Q000209:etiology / Q000517:prevention & control; D004848:Epithelium / Q000382:microbiology; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D016496:Incontinence Pads / Q000009:adverse effects; D007223:Infant; D007224:Infant Care; D007778:Lactobacillus / Q000502:physiology*; D008855:Microscopy, Electron, Scanning; D012772:Shock, Septic / Q000209:etiology / Q000517:prevention & control; D013211:Staphylococcus aureus / Q000254:growth & development*; D013630:Tampons, Surgical / Q000009:adverse effects; D014566:Urogenital System / Q000382:microbiology