Fluorescent estimation on the effect of Ca2+ antagonists on the oxidative metabolism in dissociated mammalian brain neurons.
Effect of organic Ca2+ antagonists on the oxidative metabolism or the formation of reactive oxygen species was estimated on dissociated mammalian neurons using a flow cytometer and 2',7'-dichlorofluorescin which is oxidized to be fluorescent by cellular oxidants. Of the organic Ca2+ antagonists used, flunarizine and nifedipine decreased the intensity of fluorescence at 1 microM or more while it was not the case for verapamil and diltiazem, suggesting one of the favourable actions of flunarizine and nifedipine on ischemic brain damage.
Division of Pharmacological Science, Faculty of Integrated Arts and Sciences, University of Tokushima, Japan.
Y
Y
Oyama
A
A
Hayashi
T
T
Ueha
L
L
Chikahisa
K
K
Furukawa
D000818:Animals; D001921:Brain / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D002121:Calcium Channel Blockers / Q000494:pharmacology*; D005434:Flow Cytometry; D005452:Fluoresceins; D066298:In Vitro Techniques; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D010084:Oxidation-Reduction; D051381:Rats; D017208:Rats, Wistar; D013050:Spectrometry, Fluorescence
D002121:Calcium Channel Blockers; D005452:Fluoresceins; C037631:2',7'-dichlorofluorescein