Molecular characterization of Limulus polyphemus C-reactive protein. II. Asparagine-linked oligosaccharides.
The N-linked oligosaccharides of C-reactive protein (CRP) from the arachnid Limulus polyphemus, the horseshoe crab, were characterized after their release by hydrazinolysis, re-N-acetylation, and reduction with NaB3H4. High-voltage paper electrophoresis of the reduced oligosaccharides revealed only neutral species. Gel-permeation chromatography on Bio-Gel P4 yielded five fractions. The oligosaccharide fractions were further fractionated using high-voltage borate paper electrophoresis and Dionex BioLC ion-exchange chromatography. The oligosaccharides were structurally characterized by sequential exoglycosidase digestion, fragmentation by acetolysis and methylation analysis. Three major structures were found, of which two were the biantennary oligomannose type with compositions Man5GlcNAc2 (B-1), Man4GlcNAc2 (C-3) and one was the monoantennary structure Man3GlcNAc2 (D-1). The biantennary oligomannose structures B-1 and C-3 contained the structural unit Man alpha 6Man alpha 6R. This unusual arrangement of mannose linkages suggests a biosynthetic pathway in Limulus which differs from that reported in mammals, plants and the parasitic protozoa.
Department of Biochemistry, University of Oxford, England.
S
S
Amatayakul-Chantler
R A
RA
Dwek
G A
GA
Tennent
M B
MB
Pepys
T W
TW
Rademacher
D000818:Animals; D001216:Asparagine / Q000737:chemistry*; D001665:Binding Sites; D002097:C-Reactive Protein / Q000737:chemistry* / Q000302:isolation & purification; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004586:Electrophoresis; D006737:Horseshoe Crabs / Q000737:chemistry*; D008361:Mannosidases / Q000378:metabolism; D008745:Methylation; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry* / Q000302:isolation & purification
D009844:Oligosaccharides; D001216:Asparagine; D002097:C-Reactive Protein; D008361:Mannosidases