Persistent changes in protein synthesis by vagina of ovariectomized mice exposed neonatally to diethylstilbestrol.
Neonatal treatment of female mice with a synthetic estrogen, diethylstilbestrol (DES), results in adenosis, ovary-independent epithelial proliferation and cornification, and downgrowths in the vagina. Protein synthesis was examined in the vagina of 45-day-old, ovariectomized C57BL/Tw mice which had been given 5 daily injections of 2 micrograms DES or the oil vehicle alone from the day of birth, and in those of age-matched, ovariectomized mice given 3 daily injections of 0.1 microgram DES from 42 days of age. [35S]Methionine-labeled proteins of the vagina were analyzed by two-dimensional polyacrylamide gel electrophoresis. Major changes were observed in 11 proteins of the vagina in both neonatally and postnatally DES-exposed groups. Six of the 11 proteins were increased in expression, but one of the remaining proteins was decreased. In the group of neonatally DES-exposed mice alone, expressions of 4 proteins (MW. 128, 90, 46 and 44 kDa) were markedly increased. These results indicate that neonatal exposure of mice to DES induced ovary-independent, persistent alteration in the protein synthesis of the vagina.
Takamatsu
Y
Y
Graduate School of Integrated Science, Yokohama City University, Japan.
Sato
T
T
Hayashi
Y
Y
Iguchi
T
T
Takasugi
N
N
D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism*; D004054:Diethylstilbestrol / Q000633:toxicity*; D005260:Female; D051379:Mice; D008810:Mice, Inbred C57BL; D010052:Ovariectomy; D010053:Ovary / Q000502:physiology*; D014176:Protein Biosynthesis; D012741:Sexual Maturation / Q000502:physiology; D013462:Sulfur Radioisotopes; D014621:Vagina / Q000187:drug effects* / Q000378:metabolism
D013462:Sulfur Radioisotopes; D004054:Diethylstilbestrol