Partial structure of the gene encoding the 78 kDa gastrin binding protein excludes a close relationship with the peroxisomal trifunctional enzyme.
A 10.9 kb porcine genomic clone encoding nucleotides 124-732 of the cDNA for the porcine 78 kDa gastrin-binding protein has been isolated and characterized. The coding sequence is interrupted by 7 introns, which vary in length from 93 to 3000bp. The positions of the intron/exon junctions are different from the junctions in the gene encoding the rat peroxisomal trifunctional enzyme. Despite 33% amino acid sequence identity between the two proteins it is concluded that the porcine gastrin binding protein is not closely related to the rat trifunctional enzyme.
Baldwin
G S
GS
Ludwig Institute for Cancer Research, Royal Melbourne Hospital, Victoria, Australia.
Casey
A
A
Weinstock
J
J
D015094:3-Hydroxyacyl CoA Dehydrogenases / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D004247:DNA; D004746:Enoyl-CoA Hydratase / Q000235:genetics*; D005091:Exons; D005755:Gastrins / Q000378:metabolism*; D015698:Genomic Library; D007438:Introns; D007535:Isomerases / Q000235:genetics*; D063991:Mitochondrial Trifunctional Protein; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009097:Multienzyme Complexes / Q000235:genetics*; D063994:Peroxisomal Bifunctional Enzyme; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D013552:Swine
D002352:Carrier Proteins; D005755:Gastrins; D009097:Multienzyme Complexes; D004247:DNA; D015094:3-Hydroxyacyl CoA Dehydrogenases; D063991:Mitochondrial Trifunctional Protein; D004746:Enoyl-CoA Hydratase; D063994:Peroxisomal Bifunctional Enzyme; D007535:Isomerases