Thin-layer chromatographic procedures for lipid separation.
This review focuses on the thin-layer chromatographic (TLC) separation aspects of lipid analysis. Since the space limitations do not permit, the quantitative aspects of the analyses are not discussed at length although some indications of appropriate methodology and detection reagents will be indicated. Many separations carried out by TLC have the prerequisite of proper sample preparation. Using proper sample clean-up prior to TLC enables one to carry out precise separation as well as sensitive quantitation. Thus, the discussions are divided into the two main topics--sample preparation and TLC. Examples of applications are limited to those which illustrate the capabilities of the technique as well as practicability. Since there are a number of reviews in the literature, the discussions herein are focused mainly on reports after 1985.
School of Medicine, University of Pennsylvania, Philadelphia, USA.
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Touchstone
D002855:Chromatography, Thin Layer / Q000379:methods*; D006801:Humans; D008055:Lipids / Q000302:isolation & purification*
D008055:Lipids