Comparative study of different methods for extraction of Rubella haemagglutinin from infected cells BHK-21.
The results from a comparative study of some methods for extraction of Rubella haemagglutinin from infected BHK-21 cells are presented. The best haemagglutinin extraction was achieved after the cells were treated with Tween 80-ether. The combined application of alkaline extraction and Tween 80-ether treatment also gave good results.
Research Institute of Infectious and Parasitic Diseases, Medical Academy, Sofia.
A
A
Mihailov
L
L
Kavaklova
D
D
Praskov
V
V
Jukovetz
T
T
Zaikova
D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured / Q000382:microbiology; D006224:Cricetinae; D005069:Evaluation Studies as Topic; D006386:Hemagglutination Tests; D006389:Hemagglutinins, Viral / Q000302:isolation & purification*; D012409:Rubella / Q000382:microbiology*; D012412:Rubella virus / Q000276:immunology*; D012692:Serial Passage; D014773:Virology / Q000379:methods
D006389:Hemagglutinins, Viral