Fluorescent in situ hybridization assessment of chromosome 7 copy number in uncultured lung and kidney cells.
Trisomy 7 is common in cells cultured from nonneoplastic lung and kidney tissues, but the frequency of trisomy 7 in uncultured lung and kidney has not been determined. In this study, we used fluorescent in situ hybridization (FISH) to assess chromosome 7 copy number in uncultured interphase nuclei from lung and kidney specimens. All specimens had low level mosaicism for trisomy 7(1.5-5.25%), but control experiments indicated a potential wide margin of error in quantifying these events.
Naeem
R
R
Department of Pathology, Brigham and Women's Hospital, Boston, MA 02115.
Donovan
K
K
Corson
J M
JM
Fletcher
J A
JA
D000368:Aged; D002897:Chromosomes, Human, Pair 7; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007223:Infant; D007668:Kidney / Q000473:pathology*; D008168:Lung / Q000473:pathology*; D008875:Middle Aged; D014314:Trisomy