Reduction of tobacco mosaic virus accumulation in transgenic plants producing non-functional viral transport proteins.
Transgenic plants producing the 30K temperature-sensitive transport protein (TP) of tobacco mosaic virus (TMV) mutant Ni2519 (affecting cell-to-cell transport) were found to: (i) be susceptible to wild-type TMV U1 at 24 degrees C (a permissive temperature for Ni2519 TP), (ii) acquire a certain level of resistance to TMV U1 accumulation when maintained at 33 degrees C (a non-permissive temperature for Ni2519 TP) and (iii) lose the resistance to wild-type TMV after their transfer from 33 degrees C to 24 degrees C. It is suggested that reversible temperature-dependent conformational changes in Ni2519 TP are responsible for these phenomena and that production of a TP which is only partially functional in transgenic plants confers on these plants a resistance to the virus owing to reduction of the level of cell-to-cell transport. Transgenic tobacco plants producing the 32K TP of brome mosaic virus (BMV) acquired resistance to TMV U1 suggesting that BMV TP is partially functional in tobacco plants.
Malyshenko
S I
SI
Department of Virology, Moscow State University, Russia.
Kondakova
O A
OA
Nazarova JuV
Kaplan
I B
IB
Taliansky
M E
ME
Atabekov
J G
JG
D001692:Biological Transport; D002478:Cells, Cultured; D003001:Cloning, Molecular; D006358:Hot Temperature; D009029:Mosaic Viruses / Q000254:growth & development; D009154:Mutation; D053830:Plant Viral Movement Proteins; D030821:Plants, Genetically Modified / Q000382:microbiology*; D010947:Plants, Toxic; D011523:Protoplasts / Q000382:microbiology; D014026:Nicotiana / Q000235:genetics / Q000382:microbiology*; D014027:Tobacco Mosaic Virus / Q000254:growth & development*; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism
D053830:Plant Viral Movement Proteins; D014764:Viral Proteins