Development and validation of a bioanalytical method for the quantification of diltiazem and desacetyldiltiazem in plasma by capillary zone electrophoresis.
A CZE method for the quantification of diltiazem and desacetyldiltiazem in plasma was developed and validated. Separation was accomplished at pH 2.5 in a 0.044 M phosphate buffer. Sample preparation was performed by liquid-liquid extraction and no interferences with plasma compounds were detected. The calibration graph is linear over the range 5-250 ng/ml with verapamil as internal standard. The precision and accuracy are better than 13% at 5 ng/ml, and better than 10% between 10 and 250 ng/ml. The long-term reliability of the CZE system was checked over a 3-month period. The CZE method is a useful alternative to the already established HPLC method.
3M Medica, Analytical Development, Borken, Germany.
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D002121:Calcium Channel Blockers / Q000097:blood*; D004110:Diltiazem / Q000031:analogs & derivatives* / Q000097:blood*; D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D012015:Reference Standards; D015203:Reproducibility of Results; D014700:Verapamil / Q000097:blood
D002121:Calcium Channel Blockers; C037769:deacetyldiltiazem; D014700:Verapamil; D004110:Diltiazem