MHC recognition in colonization of the thymus by bone marrow cells.
The role of major histocompatibility complex (MHC) class I and II molecules in the process of colonization of the thymic microenvironment by lymphohemopoietic cells was analyzed in an in vitro experimental model. When lymphoid-depleted fetal thymus (FT) explants were cocultured with a mixture of bone marrow (BM) cells, from donors syngeneic and allogeneic to the FT, the cells syngeneic to the FT showed a developmental preference. Treatment of these cocultures with antibodies to MHC class I (H-2D, H-2K) or class II (I-E, I-A) molecules of the syngeneic cells led to preferential development of the allogeneic donor type cells. Incubation of either the FT or the BM cell inoculum with the antibodies prior to coculture indicated that the effect was exerted on the BM cells rather than on the thymic stroma.
Department of Cell Biology, Weizmann Institute of Science, Rehovot, Israel.
M
M
Fridkis-Hareli
R
R
Eren
A
A
Sharp
L
L
Abel
T
T
Kukulansky
A
A
Globerson
D000818:Animals; D001854:Bone Marrow Cells; D002465:Cell Movement; D002478:Cells, Cultured; D006649:Histocompatibility Antigens / Q000276:immunology*; D008285:Major Histocompatibility Complex; D051379:Mice; D008815:Mice, Inbred Strains; D009924:Organ Culture Techniques; D013950:Thymus Gland / Q000166:cytology*
D006649:Histocompatibility Antigens