Effect of adherence to plastic on peripheral blood monocyte and alveolar macrophage chemiluminescence.
The chemiluminescence of peripheral blood monocytes and alveolar macrophages was determined in the presence of luminol and lucigenin, either before or after the cell adherence to the luminometer curvettes. In the case of monocytes, cell adherence induces an increase of luminol-dependent chemiluminescence and has almost no effect on the lucigenin-dependent chemiluminescence. However, it shows a strong inhibition of the lucigenin-dependent chemiluminescence and almost no effect on luminol-dependent chemiluminescence, in the case of alveolar macrophages. These results show that adhesion to plastic alters the metabolic burst of both monocytes and alveolar macrophages. Although the mechanisms are poorly understood, they seem to be related to the modifications that take place during the differentiation of peripheral monocytes to alveolar macrophages.
Clinical Chemistry, Policlinico Gemelli, Università Cattolica del Sacro Cuore, Roma, Italy.
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P
De Sole
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Fresu
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L
Frigieri
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Pagliari
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De Simone
C
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Guerriero
D000166:Acridines; D001992:Bronchoalveolar Lavage Fluid; D002448:Cell Adhesion; D002478:Cells, Cultured; D006801:Humans; D007700:Kinetics; D008163:Luminescent Measurements; D008165:Luminol; D008171:Lung Diseases / Q000097:blood / Q000503:physiopathology; D016676:Macrophages, Alveolar / Q000502:physiology*; D008875:Middle Aged; D009000:Monocytes / Q000502:physiology*; D010969:Plastics; D012507:Sarcoidosis / Q000097:blood / Q000503:physiopathology; D013997:Time Factors
D000166:Acridines; D010969:Plastics; C033472:10,10'-dimethyl-9,9'-biacridinium; D008165:Luminol