Antigenic behaviors of two axial flagellar proteins detected in Treponema denticola.
Two polypeptide antigens with molecular sizes of 34,000 daltons (34 kDa) and 38 kDa were separated from heated cells of a human clinical treponeme strain G7201 and Treponema denticola ATCC 35404, respectively. The rabbit polyclonal antisera against these antigens were produced and examined for their immunological reactions with the two heated antigens or intact spirochetal cells. Immunoblot analysis showed that the 34-kDa protein was also detected in T. denticola ATCC 35404 and ATCC 33520, and the 38-kDa protein was detected only in the two ATCC strains. Immunoelectron microscopy using the two rabbit antisera and protein A-gold complexes demonstrated that the 38-kDa protein antigen was present on the axial flagella of two T. denticola strains, and that the 34-kDa protein was located in the axial flagella of the G7201 cell, but neither in axial flagella nor on outer envelopes of the two ATCC strains cells, suggesting that the native 34-kDa axial flagellar protein of the G7201 strain may be different from that of T. denticola in terms of immunological reactivity.
Department of Oral Microbiology, School of Dentistry, Asahi University, Gifu, Japan.
T
T
Umemoto
A
A
Wadood
Y
Y
Nakamura
Y
Y
Nakatani
I
I
Namikawa
D000942:Antigens, Bacterial / Q000276:immunology*; D001426:Bacterial Proteins / Q000276:immunology*; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D005407:Flagella / Q000276:immunology* / Q000648:ultrastructure; D006801:Humans; D015151:Immunoblotting; D016253:Microscopy, Immunoelectron; D014208:Treponema / Q000276:immunology* / Q000648:ultrastructure
D000942:Antigens, Bacterial; D001426:Bacterial Proteins