Characterization of avian reovirus-induced cell fusion: the role of viral structural proteins.
Cell fusion induced by avian reovirus was analyzed using virus strain FC and Vero cells. One-step growth curves showed that cell fusion was directly associated with viral replication. Cell fusion occurred most efficiently at basic pH (8.0-8.5) and fusion from without could not be demonstrated. Actinomycin D, at low concentrations, increased cell fusion, and cycloheximide prevented cell fusion, indicating that viral protein(s) were responsible for the induction of cell fusion. Immunofluorescence tests indicated that viral proteins were present on the infected cell surface. Radioimmuno-precipitation identified structural proteins mu 2C and sigma 2 as predominant viral protein species present on the infected cell surface. Cell fusion was inhibited by virus-specific antisera, suggesting that mu 2C and/or sigma 2 present on the infected cell surface were involved in the induction of cell fusion. Trypsin and chymotrypsin treatment of purified viruses cleaved both mu 2C and sigma 2 proteins, but generated different cleavage products with each protein. The addition of trypsin to the culture media following infection increased cell fusion, whereas chymotrypsin treatment decreased cell fusion. The opposite effects of trypsin and chymotrypsin on the cell fusion, together with the different specificities of these two proteases in cleavage of mu 2C and sigma 2 proteins, further suggest that the cell surface-associated mu 2C and/or sigma 2 are involved in the syncytium formation.
Ni
Y
Y
Division of Molecular Virology, Baylor College of Medicine, Houston, Texas 77030.
Ramig
R F
RF
D000468:Alkalies; D000818:Animals; D000914:Antibodies, Viral / Q000494:pharmacology; D002459:Cell Fusion / Q000187:drug effects / Q000502:physiology*; D002645:Chickens / Q000382:microbiology; D002918:Chymotrypsin / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D008565:Membrane Proteins / Q000378:metabolism; D012087:Reoviridae / Q000187:drug effects / Q000254:growth & development*; D014357:Trypsin / Q000494:pharmacology; D014709:Vero Cells; D014760:Viral Fusion Proteins / Q000187:drug effects / Q000378:metabolism*; D010948:Viral Plaque Assay; D015678:Viral Structural Proteins / Q000187:drug effects / Q000378:metabolism*; D014771:Virion / Q000502:physiology; D014779:Virus Replication
D000468:Alkalies; D000914:Antibodies, Viral; D008565:Membrane Proteins; D014760:Viral Fusion Proteins; D015678:Viral Structural Proteins; D003609:Dactinomycin; D002918:Chymotrypsin; D014357:Trypsin