Fluorographic study on the oxidative stress in the process of gastric mucosal injury: attenuating effect of vitamin E.
In vivo oxidative change was visualized in the gastric mucosa of rats and the alteration was analysed by using a fluorescence microscope equipped with a digital imaging processor during the development of mucosal damage. Dichlorofluorescein (DCF)-associated fluorescence increased after the repeated electrical stimulation on the gastric artery (irritation), suggesting the occurrence of lipid peroxidation. The increase was enhanced in the mid-zone of two adjacent collecting venules. Allopurinol attenuated the oxidative stress in mucosa, showing the involvement of xanthine oxidase. Luminol-dependent chemiluminescence value in the blood taken from gastric vein was elevated by the irritation, suggesting that leucocyte-generated oxygen radicals also participate in this oxidative process. alpha-Tocopherol attenuated both the DCF activation and the increase in chemiluminescence value and prevented gastric mucosal injury. The present results suggest that alpha-tocopherol may be useful for the prevention of oxidative alteration in gastric mucosa.
Department of Internal Medicine, School of Medicine, Keio University, Tokyo, Japan.
I
I
Kurose
D
D
Fukumura
S
S
Miura
M
M
Suematsu
M
M
Suzuki
E
E
Sekizuka
H
H
Nagata
T
T
Morishita
M
M
Tsuchiya
D000493:Allopurinol / Q000494:pharmacology; D000818:Animals; D001783:Blood Flow Velocity; D004558:Electric Stimulation; D005453:Fluorescence; D005609:Free Radicals; D005753:Gastric Mucosa / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology*; D007962:Leukocytes / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008163:Luminescent Measurements; D008297:Male; D010084:Oxidation-Reduction; D051381:Rats; D017208:Rats, Wistar; D014810:Vitamin E / Q000494:pharmacology*; D014969:Xanthine Oxidase / Q000378:metabolism
D005609:Free Radicals; D014810:Vitamin E; D000493:Allopurinol; D014969:Xanthine Oxidase