Phospholipase-resistant phosphatidylcholine reduces intra-abdominal adhesions induced by bacterial peritonitis.
The majority of intra-abdominal adhesions develop postoperatively or following peritonitis. We have previously shown that L-phosphatidylcholine reduces postoperative peritoneal adhesions in rats. In the present study, we examined whether adhesion formation after bacterial peritonitis is also reduced by L-phosphatidylcholine or by DL-alpha-phosphatidylcholine, which is degraded only 50% by phospholipase A2. Peritonitis was induced in the rat by caecal ligation and double puncture; cecotomy was performed 12, 15, or 18 h later. Adhesions were assessed blindly by a scoring system 7 days after cecotomy. When cecotomy was scheduled for 18 h after caecal ligation and puncture, the 7-day mortality was 90% (n = 20). When cecotomy was performed at 12 h, no mortality was seen; however, the adhesion score was low (2.3 +/- 0.7). When cecotomy was performed 15 h after caecal ligation and puncture, the mortality was 25% and the adhesion score was 4.3 +/- 0.9. This figure was reduced significantly by intraperitoneal instillation of L-phosphatidylcholine or DL-alpha-phosphatidylcholine for 3 subsequent days. However, the mortality increased by L-phosphatidylcholine (P < 0.01), whereas mortality after DL-alpha-phosphatidylcholine remained at 30%. We conclude that administration of both L-phosphatidylcholine and DL-alpha-phosphatidylcholine decrease adhesion formation after bacterial peritonitis.
Department of Surgery, Lund University, Sweden.
M
M
Snoj
A
A
Ar'Rajab
B
B
Ahrén
K
K
Larsson
S
S
Bengmark
D000818:Animals; D001424:Bacterial Infections / Q000150:complications; D002431:Cecostomy; D007410:Intestinal Diseases / Q000209:etiology / Q000517:prevention & control*; D008297:Male; D010538:Peritonitis / Q000150:complications*; D010713:Phosphatidylcholines / Q000008:administration & dosage / Q000627:therapeutic use*; D010741:Phospholipases A; D054467:Phospholipases A2; D051381:Rats; D017207:Rats, Sprague-Dawley; D000267:Tissue Adhesions / Q000209:etiology / Q000517:prevention & control
D010713:Phosphatidylcholines; D010741:Phospholipases A; D054467:Phospholipases A2