Transcription rates and transcript stabilities of macronuclear genes in vegetative Euplotes crassus cells.
In hypotrichous ciliates such as Euplotes crassus genes in the transcriptionally active macronucleus are present on individual minichromosomes which occur in gene-specific copy numbers. This different degree of gene amplification can be understood as a means to preset the expression potential of the respective genetic information. In addition, the actual steady state transcript amounts are governed by the transcription rates and transcript stabilities. To establish the relative effects of these three parameters the copy numbers of genes transcribed by the three different polymerases were determined. The transcript levels of growing or starving vegetative cells were then determined, and nuclear run-on assays were performed to determine the transcription rates of the genes in the different nutritional states. A weak correlation between the gene copy numbers and transcription rates was found. The transcripts of genes synthesized by RNA polymerase II exhibited different stabilities upon starvation of the cells, compared to the supposedly stable ribosomal 5S and 26S RNA. Refeeding of the cells after starvation also resulted in a differential response with respect to the accumulation of the transcripts of different genes transcribed by RNA polymerase II, which can be interpreted in the context of the gene functions.
Brandt
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Department of Biology, Phillipps-University, Marburg, Germany.
Klein
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D000818:Animals; D001483:Base Sequence; D005075:Biological Evolution; D003227:Conjugation, Genetic; D017124:Conserved Sequence; D015342:DNA Probes; D016054:DNA, Protozoan / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D016812:Euplotes / Q000235:genetics* / Q000502:physiology*; D015870:Gene Expression; D017125:Genes, Protozoan; D012319:RNA Polymerase II / Q000378:metabolism*; D016053:RNA, Protozoan / Q000096:biosynthesis / Q000235:genetics; D012335:RNA, Ribosomal / Q000096:biosynthesis* / Q000235:genetics; D012341:RNA, Ribosomal, 5S / Q000096:biosynthesis* / Q000235:genetics; D014158:Transcription, Genetic; D014404:Tubulin / Q000096:biosynthesis
D015342:DNA Probes; D016054:DNA, Protozoan; D004275:DNA, Ribosomal; D016053:RNA, Protozoan; D012335:RNA, Ribosomal; D012341:RNA, Ribosomal, 5S; C065096:RNA, ribosomal, 26S; D014404:Tubulin; D012319:RNA Polymerase II