Applicability of flow cytometry in breast cancer.
The cumulative evidence indicates that flow cytometry can provide important information about the behavior of breast tumors. A particularly appealing aspect of this technology is the ability to determine flow cytometric parameters on fresh surgical samples, frozen biopsy specimens, and archival paraffin-embedded blocks. However, several issues regarding the standardization of tissue preparation and the cell cycle analyses must be addressed before flow cytometry can be routinely applied on a wide scale. Quality control standards are currently being discussed that will help to reduce interlaboratory variability, particularly with respect to absolute values of S-phase fraction.
Department of Medicine/Oncology, University of Texas Health Science Center, San Antonio 78284-7884.
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Clark
D001943:Breast Neoplasms / Q000235:genetics / Q000473:pathology*; D002453:Cell Cycle; D002455:Cell Division; D004273:DNA, Neoplasm / Q000032:analysis; D005260:Female; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D009362:Neoplasm Metastasis; D011003:Ploidies; D011379:Prognosis; D012008:Recurrence; D016196:S Phase
D004273:DNA, Neoplasm