Avian reovirus proteins associated with neutralization of virus infectivity.
Monoclonal antibodies against two virion proteins of the RAM-1 strain of avian reovirus neutralized virus infectivity; antibody against a 124-kDa (lambda B) protein caused broadly specific neutralization and antibody against a 39-kDa (sigma C) protein caused neutralization of greater type-specificity. The neutralizing activity of the monoclonals also exhibited host cell specificity: antibodies against the lambda B protein inhibited virus infectivity in Vero cells and not chicken kidney cells; one monoclonal antibody against the sigma C protein neutralized virus in only chicken kidney cells, whereas two other monoclonals against the sigma C protein neutralized virus in both Vero and chicken kidney cells but had greater neutralizing activity in Vero cells.
School of Veterinary Studies, Murdoch University, Australia.
R
R
Wickramasinghe
J
J
Meanger
C E
CE
Enriquez
G E
GE
Wilcox
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology*; D002478:Cells, Cultured; D002645:Chickens / Q000382:microbiology*; D014644:Genetic Variation; D009500:Neutralization Tests; D012087:Reoviridae / Q000276:immunology* / Q000472:pathogenicity; D012703:Serotyping; D013045:Species Specificity; D014764:Viral Proteins / Q000276:immunology*; D014774:Virulence
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D014764:Viral Proteins