Analysis of human sera for filarial IgM antibody using antigen fractions isolated from immune complexes.
Filarial IgM antibody response to various antigenic fractions isolated from immune complexes by SDS-PAGE viz., IC-2, IC-4, IC-7, and IC-9, was studied by ELISA. The IgM antibody response to almost all antigenic fractions was seen more in microfilaraemic group compared to the clinical filariasis group. The antibody response to IC-9 antigen fraction as compared to other fractions was found significantly higher in microfilaraemic patients than in patients with clinical manifestations. Ninety percent of microfilariae carriers and 75% of clinical filarial patients have shown IgM isotype to IC-9 antigen. Studies with various lectins have revealed the Con A binding nature of IC-9 antigen. This study suggests that IC-9 antigen could be another candidate antigen in the immunodiagnosis of lymphatic filariasis.
Prasad
G B
GB
School of Life Sciences, Ravishankar University, Raipur, M.P., India.
Harinath
B C
BC
D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D000936:Antigen-Antibody Complex / Q000276:immunology*; D000947:Antigens, Helminth / Q000276:immunology*; D004605:Elephantiasis, Filarial / Q000175:diagnosis; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007075:Immunoglobulin M / Q000097:blood*; D014958:Wuchereria bancrofti / Q000276:immunology*
D000909:Antibodies, Helminth; D000936:Antigen-Antibody Complex; D000947:Antigens, Helminth; D007075:Immunoglobulin M