Kinetics of serum and ocular antibody responses in susceptible mice that received a secondary corneal infection with Pseudomonas aeruginosa.
When susceptible C57BL/6J mice were infected with Pseudomonas aeruginosa in one eye and then reinfected in the previously uninfected contralateral control eye either 4 or 8 weeks after the primary infection, approximately 20 to 30% of the mice receiving a 4-week reinfection regimen restored corneal clarity within 4 weeks, while almost all of the 8-week-reinfected mice restored corneal clarity within 3 to 6 days postinfection. However, the rate of bacterial clearance was the same in both sets of mice despite the presence of opsonophagocytic antibodies only in the 8-week-reinfected mice. As determined by enzyme-linked immunosorbent assay, immunoglobulin G was the major immunoglobulin in both serum and ocular tissue of both mouse sets, and the immunoglobulin G level was two- to fourfold higher after the 8-week secondary infection than in the 4-week-reinfected mice.
Department of Immunology, Wayne State University School of Medicine, Detroit, Michigan 48201.
M J
MJ
Preston
K
K
Kernack
R S
RS
Berk
D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis* / Q000097:blood; D003315:Cornea / Q000276:immunology / Q000382:microbiology; D003316:Corneal Diseases / Q000276:immunology*; D004198:Disease Susceptibility; D005123:Eye / Q000276:immunology* / Q000382:microbiology; D007074:Immunoglobulin G / Q000096:biosynthesis; D007700:Kinetics; D051379:Mice; D008810:Mice, Inbred C57BL; D009895:Opsonin Proteins / Q000097:blood; D011552:Pseudomonas Infections / Q000276:immunology*; D011550:Pseudomonas aeruginosa / Q000276:immunology / Q000302:isolation & purification; D013666:Tears / Q000276:immunology
D000907:Antibodies, Bacterial; D007074:Immunoglobulin G; D009895:Opsonin Proteins