Mesangial cell proliferation is directly enhanced by sera from patients with renal insufficiency.
We undertook the present study to determine whether there is a direct effect of sera from patients with renal insufficiency on mesangial cell proliferation. Growth-arrested mesangial cells were incubated with sera from patients with varying degrees of renal insufficiency and then pulsed with [3H]thymidine. Thymidine uptake was progressively greater with more advanced renal failure and was linearly correlated with (creatinine)-1. This data suggests that serum from patients with loss of renal function contains mitogens capable of directly enhancing mesangial cell proliferation.
Department of Medicine, Long Island Jewish Medical Center, New Hyde Park, NY 11042.
J
J
Mattana
M
M
Abramovici
P C
PC
Singhal
D000818:Animals; D002455:Cell Division / Q000187:drug effects*; D002478:Cells, Cultured; D003404:Creatinine / Q000008:administration & dosage / Q000097:blood; D004305:Dose-Response Relationship, Drug; D005920:Glomerular Mesangium / Q000166:cytology* / Q000378:metabolism*; D006801:Humans; D051381:Rats; D017207:Rats, Sprague-Dawley; D051437:Renal Insufficiency / Q000097:blood*; D013936:Thymidine / Q000008:administration & dosage / Q000378:metabolism; D014508:Urea / Q000008:administration & dosage / Q000097:blood*
D014508:Urea; D003404:Creatinine; D013936:Thymidine