Conformational recognition of RA-XII by 80S ribosomes: a differential line broadening study in 1H NMR spectroscopy.
1H NMR spectroscopy has been used to demonstrate specific binding of rat 80S ribosomes to the major conformer of an antitumor bicyclic hexapeptidic glucoside, RA-XII, isolated from Rubia cordifolia, in a fast exchange process.
Morita
H
H
Tokyo College of Pharmacy, Japan.
Yamamiya
T
T
Takeya
K
K
Itokawa
H
H
Sakuma
C
C
Yamada
J
J
Suga
T
T
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000378:metabolism*; D066298:In Vitro Techniques; D009682:Magnetic Resonance Spectroscopy; D008968:Molecular Conformation; D010456:Peptides, Cyclic / Q000378:metabolism*; D051381:Rats; D012270:Ribosomes / Q000378:metabolism*
D000972:Antineoplastic Agents, Phytogenic; D010456:Peptides, Cyclic; C081563:RA XII