Effect of cooked meat on micronucleus frequency.
The possibility that inclusion of cooked meat in the diet could elevate chromosome damage risk was investigated in mice fed either a cereal diet only or a cereal diet supplemented with mutton cooked by microwaving, pan-frying or charcoal-barbecueing. The meat was provided as a supplement to the standard cereal-based diet on an approximately one-to-one basis. Chromosome damage indices included the cytokinesis-block micronucleus assay in spleen lymphocytes, the bone marrow micronucleus assay in erythrocytes and two versions of the colonic micronucleus assay. These indices were assessed after 3 or 6 months of feeding, and under each circumstance no increment in the micronucleus frequency in any of the tissues could be detected in the groups fed cooked meat when compared with each other or with the group fed the cereal diet only. There was also no correlation between the micronucleus index in lymphocytes and body weight. These data suggest that, over the time-frame studied, inclusion of meat in the diet and the extent to which meat is cooked are unlikely to be important variables affecting the micronucleus index.
CSIRO Division of Human Nutrition, Adelaide, South Australia.
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Fenech
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Neville
D000818:Animals; D001835:Body Weight / Q000187:drug effects; D001853:Bone Marrow / Q000187:drug effects*; D003106:Colon / Q000187:drug effects; D003296:Cooking / Q000379:methods*; D004044:Dietary Proteins / Q000494:pharmacology*; D005260:Female; D008214:Lymphocytes / Q000187:drug effects; D008460:Meat; D051379:Mice; D015162:Micronucleus Tests; D013154:Spleen / Q000187:drug effects*
D004044:Dietary Proteins