[The effect of blood cell status on cytochemical reactions].
Using luminescence microspectroscopy, it was shown that the cytochemical reaction between acridine orange and nucleic acids of fixed blood cells in smears depends on the physical state of these cells during the study. On fixation, the cellular membrane transforms into a semipermeable envelope thus causing an increase in size and light scattering of cells under the action of osmotic pressure of solvents used to wash the preparations. As a consequence, the migration of energy from monomers to dimers increases, which should be taken into account when interpreting the data on the cytochemical reaction.
N A
NA
Karnaukhova
L A
LA
Sergievich
D000818:Animals; D001402:B-Lymphocytes / Q000737:chemistry*; D006651:Histocytochemistry; D008027:Light; D049449:Luminescence; D011817:Rabbits; D012542:Scattering, Radiation; D013057:Spectrum Analysis / Q000379:methods; D013601:T-Lymphocytes / Q000737:chemistry*
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