Direct ABC immunohistochemistry to Encephalitozoon cuniculi.
Among the sera from 9 rabbits spontaneously infected with Encephalitozoon cuniculi, a serum which revealed a high titer for E. cuniculi by indirect protein A-gold (IPAG) immunohistochemistry and reacted with the outer layer of the shell of E. cuniculi spores on immunoelectron microscopical examination, was biotinylated. The biotinylated rabbit anti-E. cuniculi IgG reacted immunohistochemically with E. cuniculi, but not with other protozoa tested, namely Neospora caninum, Toxoplasma gondii and sarcocystis. The direct avidin-biotin-peroxidase complex (ABC) immunohistochemistry using biotinylated rabbit anti-E. cuniculi IgG in this study is a useful tool for the diagnosis and study of encephalitozoonosis.
Department of Comparative Pathology, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
J H
JH
Park
K
K
Ochiai
C
C
Itakura
D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D001921:Brain / Q000469:parasitology* / Q000473:pathology; D002540:Cerebral Cortex / Q000469:parasitology / Q000473:pathology / Q000648:ultrastructure; D009670:Encephalitozoon cuniculi / Q000302:isolation & purification*; D016890:Encephalitozoonosis / Q000097:blood / Q000473:pathology / Q000662:veterinary*; D007074:Immunoglobulin G / Q000097:blood*; D007150:Immunohistochemistry; D007668:Kidney / Q000469:parasitology* / Q000473:pathology; D016253:Microscopy, Immunoelectron; D011817:Rabbits
D000913:Antibodies, Protozoan; D007074:Immunoglobulin G