Transcriptional analysis of the Streptomyces glaucescens tetracenomycin C biosynthesis gene cluster.
A 12.6-kb DNA fragment from Streptomyces glaucescens GLA.0 containing the 12 genes for tetracenomycin (TCM) C biosynthesis and resistance enabled Streptomyces lividans to produce TCM C. Transcriptional analysis of the tcmPG intergenic region in this cluster established the presence of two divergent promoters. The tcmIc mutation, a T-to-G transversion in the -10 region of the tcmG promoter, decreased promoter activity drastically at the stationary growth stage and time of maximum TCM C accumulation. This promoter may direct the transcription of a tcmGHIJKLMNO operon, while the other promoter is for tcmP.
School of Pharmacy, University of Wisconsin, Madison 53706.
H
H
Decker
C R
CR
Hutchinson
D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D001483:Base Sequence; D016384:Consensus Sequence; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D005810:Multigene Family; D009279:Naphthacenes / Q000378:metabolism; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D009876:Operon; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics; D015183:Restriction Mapping; D013302:Streptomyces / Q000235:genetics*; D014158:Transcription, Genetic
D000900:Anti-Bacterial Agents; D009279:Naphthacenes; D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger; C020938:tetracenomycin C