Mössbauer spectroscopy of metalloproteins and the use of Fourier transforms.
A method for obtaining accurate, quantitative 57Fe Mössbauer spectra from biological samples is illustrated stepwise in a data reduction procedure. Exact criteria are presented for deciding when it is necessary to account for the effects of the Beer-Lambert law in the Mössbauer spectra from biological samples. This procedure makes extensive use of the fast Fourier transform and other computer techniques in its data reduction and its curve-fitting stages. A method for optimizing sample thickness is presented. The choice of truncation in Fourier space as a means to numerically stabilize the deconvolution procedure is defended. Several advantages for curve fitting in Fourier space are shown. Maximization of information content is discussed for Mossbauer spectral simulation techniques.
Dunham
W R
WR
Biophysics Research Division, University of Michigan, Ann Arbor 48109.
Harding
L J
LJ
Sands
R H
RH
D005583:Fourier Analysis; D008667:Metalloproteins / Q000737:chemistry*; D015204:Spectroscopy, Mossbauer
D008667:Metalloproteins