Activation of prophenoloxidase with 2-propanol and other organic compounds in Drosophila melanogaster.
Activation with 2-propanol and other organic compounds of prophenoloxidase purified from pupae of Drosophila melanogaster was analyzed. A1, one of the two isozymes of the prophenoloxidase, could be activated with both an endogenous activating system and artificial organic compounds including alcohols. A1 was activated within 2 min after addition of 2-propanol. The phenoloxidase activity of A1, which had been activated with 2-propanol, decreased gradually by lowering the concentration of 2-propanol taking c 60 min to attain a low level, and the activity could be re-elevated at the re-introduction of 2-propanol. Thus the reversibility of the activation of A1 in response to the change of the concentration of 2-propanol in the activating mixture could be observed. Optimum concentration of 2-propanol for the rate of activation was 50%, optimum temperature was 30 degrees C and optimum pH was 7.5. The final level of the phenoloxidase activity, which had been activated with 2-propanol, was higher than that activated with the endogenous activating system. The activated state of A1 showed properties of a tyrosinase-type phenoloxidase. The results suggested that the activation of A1 with 2-propanol is caused by the reversible conformational change of the prophenoloxidase molecule.
N
N
Asada
T
T
Fukumitsu
K
K
Fujimoto
K
K
Masuda
D000433:1-Propanol / Q000494:pharmacology*; D000438:Alcohols / Q000494:pharmacology; D000818:Animals; D004156:Catechol Oxidase / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004331:Drosophila melanogaster / Q000201:enzymology*; D004789:Enzyme Activation / Q000187:drug effects; D004792:Enzyme Precursors / Q000187:drug effects*; D011679:Pupa / Q000201:enzymology; D013379:Substrate Specificity; D013997:Time Factors
D000438:Alcohols; D004792:Enzyme Precursors; D000433:1-Propanol; C038451:pro-phenoloxidase; D004156:Catechol Oxidase