Identification of inbred strains of mice and genetic relationships between strains as assessed by DNA fingerprinting.
Strain identification and the assessment of genetic relationships among inbred strains of mice were examined by DNA fingerprinting using M13 phage DNA as a probe. We used 8 inbred strains (NC/Jah, DDD/Jah, DSD/Jah, RR/Jah, SS/Jah, C3H/HeJah, C57BL/6 Jah and C57BL/6CrSlc) and coisogenic (NC-brp) as well as congenic (C57BL/6-bg) strains. DNA fingerprints using M13 phage DNA as a probe revealed identical patterns within strains. Bands due to new mutations in minisatellite regions within each strain were not observed. The banding patterns were strain-specific. Thus, DNA fingerprinting seemed to be suitable for genetic monitoring of mice. A dendrogram constructed with difference values calculated from DNA fingerprint patterns showed that relationships among strains reflected the history of these strains, so DNA fingerprinting could also be effectively used to assess the relationships between inbred strains of mice.
Laboratory of Animal Breeding, Faculty of Agriculture, Kobe University, Hyogo, Japan.
H
H
Mannen
S
S
Tsuji
N
N
Goto
K
K
Fukuta
D000818:Animals; D016172:DNA Fingerprinting; D005260:Female; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains / Q000145:classification / Q000235:genetics*