Biochemical remodeling of collagen in the heart of spontaneously hypertensive rats--prominent increase in type V collagen.
To determine how collagen is remodeled in the heart during the development of cardiac hypertrophy, the collagen concentration and the proportions of types I, III, and V collagen were analyzed in the hearts of 10-, 20-, 32-, and 40-week-old spontaneously hypertensive (SHR) and Wistar-Kyoto (WKY) rats. The collagen concentration was calculated after the hydroxyproline content was measured and the proportions of types I, III, and V collagen were determined by non-interrupted SDS-polyacrylamide gel electrophoresis (SDS-PAGE). There was no significant difference between the collagen concentrations in 10-week-old SHR and WKY. At 20 weeks, the collagen concentration in the hearts of the SHR had decreased significantly (p < 0.01) when compared to that in the WKY, at 40 weeks, this concentration in SHR had increased significantly (p < 0.05) when compared to that in the WKY. Type V collagen in WKY increased with age and type I collagen in the 20- and 40-week-old WKY decreased significantly (p < 0.05) when compared to that in the 10-week-old WKY. However, the proportion of type I collagen in the 20-week-old WKY did not differ from that of the 40-week-old WKY. There was no significant difference between the proportions of the various types of collagen in 10-week-old SHR and those in age- and sex-matched WKY. However, the proportion of type V collagen in the 20- and 40-week-old SHR was significantly (p < 0.01) higher than that in age- and sex-matched WKY.(ABSTRACT TRUNCATED AT 250 WORDS)
Honda
M
M
4th Department of Internal Medicine, Shimane Medical University, Izumo, Japan.
Goto
Y
Y
Kuzuo
H
H
Ishikawa
S
S
Morioka
S
S
Yamori
Y
Y
Moriyama
K
K
D000818:Animals; D003094:Collagen / Q000032:analysis* / Q000502:physiology; D003488:Cyanogen Bromide / Q000378:metabolism; D005260:Female; D006321:Heart / Q000502:physiology*; D017379:Hypertrophy, Left Ventricular / Q000503:physiopathology; D008297:Male; D009206:Myocardium / Q000737:chemistry*; D051381:Rats; D011918:Rats, Inbred SHR / Q000502:physiology*; D011921:Rats, Inbred WKY
D003094:Collagen; D003488:Cyanogen Bromide