Alteration in rat apolipoprotein C-III gene expression and lipoprotein composition during inflammation.
A cloned rat apolipoprotein (apo) C-III cDNA was used as a hybridization probe to measure apo C-III mRNA levels in rats after induction of inflammation. Hepatic apo C-III mRNA levels decrease to a minimum value of 46% of normal 72 h after the induction of inflammation. The changes observed are very similar to the changes in mRNA levels of rat apo A-IV, while the hepatic mRNA levels for apo A-I and apo C-I during inflammation remain relatively constant. Alterations in apo C-III gene expression during inflammation were associated with changes in lipoprotein particle size and composition. A decrease of approximately twofold in the amount of apo C-III-containing HDL particles was found in rats 24 h after the induction of inflammation. The average size of apo C-III-containing HDL particles in rats during inflammation is significantly larger than that of the control group. A decrease in the concentration of apo A-I-containing HDL particles was also observed in these rats. The results indicate altered apolipoprotein gene expression associated with alterations in the size and composition of HDL particles.
Russell Grimwade School of Biochemistry, University of Melbourne, Parkville, Victoria, Australia.
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Shen
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Howlett
D000818:Animals; D053302:Apolipoprotein C-I; D053305:Apolipoprotein C-III; D001056:Apolipoproteins C / Q000097:blood / Q000737:chemistry* / Q000235:genetics*; D002850:Chromatography, Gel; D015870:Gene Expression; D007249:Inflammation / Q000097:blood / Q000378:metabolism*; D008055:Lipids / Q000097:blood; D008074:Lipoproteins / Q000097:blood / Q000737:chemistry*; D008075:Lipoproteins, HDL / Q000737:chemistry; D008297:Male; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011915:Rats, Inbred BUF
D053302:Apolipoprotein C-I; D053305:Apolipoprotein C-III; D001056:Apolipoproteins C; D008055:Lipids; D008074:Lipoproteins; D008075:Lipoproteins, HDL; D012333:RNA, Messenger