Scanning electron microscopy of human sperms after preparation of semen for in-vitro fertilization.
Ultrastructural changes of human sperms after routine preparation for in-vitro fertilization were examined by scanning electron microscopy. Studies were performed with freshly ejaculated semen of 21 normozoospermic patients. Spermatozoa were analysed at 10000-fold (sperm head with acrosome, postacrosomal border and postnuclear cap) and 2500-fold (midpiece and endpiece of sperm tail) magnification. Compared with untreated specimens, slight membrane damage was found after routine washing and centrifugation procedures in swim-up preparations. However, on the basis of a score system for quantification of morphologic data, no statistically significant differences existed between untreated semen and swim-up preparations. We conclude that, with normozoospermic semen, the rate of ultrastructural damage attributable to sperm-washing procedures is too low to be of clinical consequence.
Grab
D
D
Department of Gynecology and Obstetrics, University of Ulm, Germany.
Thierauf
S
S
Rosenbusch
B
B
Sterzik
K
K
D000177:Acrosome / Q000648:ultrastructure; D005307:Fertilization in Vitro; D006801:Humans; D008297:Male; D008855:Microscopy, Electron, Scanning; D013082:Sperm Tail / Q000648:ultrastructure; D013094:Spermatozoa / Q000648:ultrastructure*