Identification of a novel group of Serpulina hyodysenteriae isolates by using a lipopolysaccharide-specific monoclonal antibody.
A monoclonal antibody to Serpulina hyodysenteriae 8930 was produced and was used to probe pronase-treated cell lysates of S. hyodysenteriae isolates in immunblots. The results showed that the monoclonal antibody was specific for only five closely related S. hyodysenteriae isolates: 8930, 5380, 70A, RMIT 88, and RMIT 97.
Department of Applied Biology, Royal Melbourne Institute of Technology, Victoria, Australia.
M R
MR
Alderton
S C
SC
Smith
P J
PJ
Coloe
D000818:Animals; D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D015373:Bacterial Typing Techniques; D017401:Brachyspira hyodysenteriae / Q000145:classification* / Q000276:immunology / Q000302:isolation & purification; D005069:Evaluation Studies as Topic; D015151:Immunoblotting; D008070:Lipopolysaccharides / Q000276:immunology*; D013145:Spirochaetales Infections / Q000382:microbiology / Q000662:veterinary; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D008070:Lipopolysaccharides