Positive patches and negative noodles: linking RNA processing to transcription?
A speculative model is presented that proposes specific mechanisms for effecting co-transcriptional splice site selection in pre-mRNAs. The model envisions that certain splicing factors containing arginine-rich, positively charged regions bind via these positive patches to the hyperphosphorylated, negatively charged tail of elongating RNA polymerase II. Thus tethered to the transcription machinery, these splicing factors gain access to signals in nascent transcripts as they emerge from the polymerase.
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Department of Biochemistry, Duke University Medical Center, Durham, NC 27710.
D000595:Amino Acid Sequence; D000818:Animals; D008957:Models, Genetic; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D012319:RNA Polymerase II / Q000737:chemistry / Q000378:metabolism*; D012322:RNA Precursors / Q000378:metabolism*; D012326:RNA Splicing; D012091:Repetitive Sequences, Nucleic Acid; D014158:Transcription, Genetic
D010446:Peptide Fragments; D012322:RNA Precursors; D012319:RNA Polymerase II