Identification and characterization of an early estrogen-regulated RNA in cultured guinea-pig endometrial cells.
A cDNA library was prepared from quiescent guinea-pig endometrial glandular epithelial cells stimulated for 2 h with estradiol-17 beta (E2) in the presence of cycloheximide. It was screened by differential hybridization for estrogen-regulated sequences. Six recombinants containing E2-regulated sequences were identified. One of them, called gec1 was then characterized by Northern blot hybridization. The gec1 mRNA was 1,800 bases in size. A 2-fold increase in the gec1 mRNA level was achieved at 120 min after E2 treatment. The E2 action on gec1 gene required the presence of cycloheximide. The cloned gec1 cDNA was 1 kb in size. The sequence so far determined did not show similarity with well characterized genes. This is the first report on a cloned cDNA probe of early estrogen-induced mRNA in a primary culture of endometrial epithelial cells.
INSERM U 198, Besançon, France.
I
I
Pellerin
C
C
Vuillermoz
M
M
Jouvenot
C
C
Ordener
M
M
Royez
G L
GL
Adessi
D000818:Animals; D015152:Blotting, Northern; D002478:Cells, Cultured; D003001:Cloning, Molecular; D003513:Cycloheximide / Q000494:pharmacology; D004717:Endometrium / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D004958:Estradiol / Q000502:physiology*; D005260:Female; D005786:Gene Expression Regulation; D015723:Gene Library; D006168:Guinea Pigs; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis* / Q000302:isolation & purification; D013997:Time Factors
D012333:RNA, Messenger; D004958:Estradiol; D003513:Cycloheximide