Purification of growth hormones by reversed-phase high-performance liquid chromatography.
Reversed-phase high-performance liquid chromatography (HPLC) on a column of Radial-Pak C18 cartridge was utilized for the purification of a variety of growth hormone (GH) proteins from mammalian, avian, amphibian and fish pituitary glands. Recovery of GH from pituitary glands of up to 0.43% of total protein was obtained with a high degree of homogeneity as revealed by sodium dodecyl sulphate polyacrylamide gel electrophoresis. The HPLC-purified GHs show reactions of identity or near identity by immuno-diffusion studies on agar gel. This method offers a convenient and rapid purification of vertebrate GH on an analytical or preparative scale.
Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan.
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D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D006041:Goats; D013006:Growth Hormone / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D005779:Immunodiffusion; D010902:Pituitary Gland / Q000737:chemistry*; D011892:Rana catesbeiana; D051381:Rats; D012756:Sheep; D013552:Swine
D000596:Amino Acids; D013006:Growth Hormone