Expression of MMP-7(PUMP-1) mRNA in human colorectal cancers.
The expression of MMP-7 (pump-1) gene was examined in 10 cases of colorectal cancer by utilizing RT-PCR. In 9 out of 10 cases, MMP-7 mRNA was detected in cancerous tissue, whereas none was detected in adjacent normal colon tissue. However, this message was detected in only 1 out of 6 colon-cancer cell lines. In colonic mucosa from 3 patients with ulcerative colitis it was not detected. The expression of MMP-2 (72-kDa type-IV collagenase) mRNA was also investigated in the same tissue samples, and was detected in all samples, including cancerous and non-cancerous tissue. Our data suggest that MMP-7 is expressed in a tumor-associated manner in colorectal cancers and may play a role in tumor progression.
Department of Internal Medicine (Section 1), School of Medicine, Sapporo Medical University, Japan.
M
M
Yoshimoto
F
F
Itoh
H
H
Yamamoto
Y
Y
Hinoda
K
K
Imai
A
A
Yachi
D000328:Adult; D000368:Aged; D001483:Base Sequence; D003106:Colon / Q000737:chemistry; D015179:Colorectal Neoplasms / Q000737:chemistry*; D005260:Female; D015870:Gene Expression; D006801:Humans; D008297:Male; D020778:Matrix Metalloproteinase 2; D020783:Matrix Metalloproteinase 7; D008666:Metalloendopeptidases / Q000032:analysis* / Q000235:genetics; D008875:Middle Aged; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis*; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
D012333:RNA, Messenger; D008666:Metalloendopeptidases; D020783:Matrix Metalloproteinase 7; D020778:Matrix Metalloproteinase 2