Reduction of tobacco mosaic virus accumulation in transgenic plants producing non-functional viral transport proteins.
Transgenic plants producing the 30K temperature-sensitive transport protein (TP) of tobacco mosaic virus (TMV) mutant Ni2519 (affecting cell-to-cell transport) were found to: (i) be susceptible to wild-type TMV U1 at 24 degrees C (a permissive temperature for Ni2519 TP), (ii) acquire a certain level of resistance to TMV U1 accumulation when maintained at 33 degrees C (a non-permissive temperature for Ni2519 TP) and (iii) lose the resistance to wild-type TMV after their transfer from 33 degrees C to 24 degrees C. It is suggested that reversible temperature-dependent conformational changes in Ni2519 TP are responsible for these phenomena and that production of a TP which is only partially functional in transgenic plants confers on these plants a resistance to the virus owing to reduction of the level of cell-to-cell transport. Transgenic tobacco plants producing the 32K TP of brome mosaic virus (BMV) acquired resistance to TMV U1 suggesting that BMV TP is partially functional in tobacco plants.
Department of Virology, Moscow State University, Russia.
S I
SI
Malyshenko
O A
OA
Kondakova
Nazarova JuV
I B
IB
Kaplan
M E
ME
Taliansky
J G
JG
Atabekov
D001692:Biological Transport; D002478:Cells, Cultured; D003001:Cloning, Molecular; D006358:Hot Temperature; D009029:Mosaic Viruses / Q000254:growth & development; D009154:Mutation; D053830:Plant Viral Movement Proteins; D030821:Plants, Genetically Modified / Q000382:microbiology*; D010947:Plants, Toxic; D011523:Protoplasts / Q000382:microbiology; D014026:Nicotiana / Q000235:genetics / Q000382:microbiology*; D014027:Tobacco Mosaic Virus / Q000254:growth & development*; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism
D053830:Plant Viral Movement Proteins; D014764:Viral Proteins