Analysis of four microsatellite markers on the long arm of chromosome 9 by meiotic recombination in flow-sorted single sperm.
Meiotic recombination in flow-sorted single sperm was used to analyze four highly polymorphic microsatellite markers on the long arm of chromosome 9. The microsatellites comprised three tightly linked markers: 9CMP1 (D9S109), 9CMP2 (D9S127), and D9S53, which map to 9q31, and a reference marker, ASS, which is located in 9q34.1. Haplotypes of single sperm were assessed by using PCR in a single-step multiplex reaction to amplify each locus. Recombinant haplotypes were identified by their relative infrequency and were analyzed using THREELOC, a maximum-likelihood-analysis program, and an adaptation of CRI-MAP. The most likely order of these markers was cen-D9S109-D9S127-D9S53-ASS-tel with D9S109, D9S127, and D9S53 being separated by a genetic distance of approximately 3%. The order of the latter three markers did not however achieve statistical significance using the THREELOC program.
Furlong
R A
RA
Department of Pathology, University of Cambridge, England.
Goudie
D R
DR
Carter
N P
NP
Lyall
J E
JE
Affara
N A
NA
Ferguson-Smith
M A
MA
D001483:Base Sequence; D002874:Chromosome Mapping; D002899:Chromosomes, Human, Pair 9; D004276:DNA, Satellite / Q000032:analysis*; D003627:Data Interpretation, Statistical; D005434:Flow Cytometry; D005819:Genetic Markers; D006239:Haplotypes; D006801:Humans; D008297:Male; D008540:Meiosis; D008969:Molecular Sequence Data; D010375:Pedigree; D016133:Polymerase Chain Reaction; D011995:Recombination, Genetic; D013094:Spermatozoa / Q000648:ultrastructure*
D004276:DNA, Satellite; D005819:Genetic Markers