Characterization of a novel dentin matrix acidic phosphoprotein. Implications for induction of biomineralization.
Acidic phosphorylated proteins have been shown to be prominent constituents of the extracellular matrix of bone and dentin. The acidic phosphoproteins of bone contain more glutamic acid than aspartic acid and a lower serine content than either. On the other hand, the major dentin acidic phosphoproteins, phosphophoryns, have been defined as aspartic acid- and serine-rich proteins, with a lesser content of glutamic acid. Both sets of phosphoproteins have been implicated as key participants in regulating mineralization, but it has been difficult to unify their mechanisms of action. We have now identified, by cDNA cloning, a new serine-rich acidic protein of the dentin matrix, AG1, with a composition intermediate between the bone acidic proteins and dentin phosphophoryns. AG1 has numerous acidic consensus sites for phosphorylation by both casein kinases I and II. Immunochemical and organ culture biosynthetic studies show that AG1 is present in phosphorylated form at low levels in the dentin matrix. If fully phosphorylated, AG1 would bear a net charge of -175/molecule of 473 residues. AG1 contains single RGD integrin binding and N-glycosylation sequences. The overall picture that emerges is that of a matrix-associated acidic phosphoprotein, with a potentially high calcium ion binding capacity, present at levels compatible with a regulatory role in dentin mineralization.
Division of Oral Biology, Northwestern University, Chicago, Illinois 60611.
A
A
George
B
B
Sabsay
P A
PA
Simonian
A
A
Veis
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002113:Calcification, Physiologic; D003001:Cloning, Molecular; D004247:DNA; D003782:Dental Pulp / Q000378:metabolism*; D016326:Extracellular Matrix Proteins; D015723:Gene Library; D007180:Incisor; D008297:Male; D008969:Molecular Sequence Data; D009804:Odontoblasts / Q000378:metabolism*; D009838:Oligodeoxyribonucleotides; D009924:Organ Culture Techniques; D009928:Organ Specificity; D010750:Phosphoproteins / Q000096:biosynthesis* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D011487:Protein Conformation; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D015183:Restriction Mapping
C081635:Dmp1 protein, rat; D016326:Extracellular Matrix Proteins; D009838:Oligodeoxyribonucleotides; D010750:Phosphoproteins; D012333:RNA, Messenger; D004247:DNA