Confocal imaging of the retrograde tracer fluoro-gold using a non-ultraviolet laser.
Until now, Fluoro-gold confocal imaging has been problematic because it requires an ultraviolet laser. We describe here a practical method to visualize Fluoro-gold in a confocal laser scanning microscope without an ultraviolet laser through an immunofluorescence procedure using antibodies against Fluoro-gold.
Neuroscience Program, University of Iowa, Iowa City 52242.
L J
LJ
Freedman
H
H
Yi
D000818:Animals; D001921:Brain / Q000166:cytology; D005456:Fluorescent Dyes / Q000032:analysis*; D007834:Lasers; D008856:Microscopy, Fluorescence / Q000379:methods*; D051381:Rats; D013266:Stilbamidines
C049774:2-hydroxy-4,4'-diamidinostilbene, methanesulfonate salt; D005456:Fluorescent Dyes; D013266:Stilbamidines