Polychlorinated biphenyl residues in food and human milk: determination of co-planar and mono-ortho substituted congeners.
An analytical method is described for determining the residues of coplanar as well as mono-ortho substituted PCB congeners both exhibiting toxicological relevance, in foods of animal origin and in human milk. The unsophisticated procedure, convenient for routine analyses, includes the extraction of lipids, clean-up steps using liquid/liquid partition and column chromatography, fractionation of the congeners using HPLC on a special carbon column with an optimal gradient elution, and capillary column gas chromatography with electron capture or mass spectrometric detection. As preliminary results indicate, the low-chlorinated PCB technical products contribute more to the actual contamination of environment and foods than has been estimated so far. Obviously the co-planar congeners are accumulated during the food chain in a portion different from that found for the other congeners.
Institut für Lebensmittelchemie, Universität Münster, Germany.
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D000818:Animals; D002417:Cattle; D005591:Chemical Fractionation; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D004350:Drug Residues / Q000032:analysis*; D005223:Fats / Q000737:chemistry; D005399:Fishes; D005504:Food Analysis / Q000379:methods; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D008892:Milk / Q000737:chemistry*; D008895:Milk, Human / Q000737:chemistry*; D011078:Polychlorinated Biphenyls / Q000032:analysis*; D046048:Seals, Earless
D005223:Fats; D011078:Polychlorinated Biphenyls