Mesangial cell proliferation is directly enhanced by sera from patients with renal insufficiency.
We undertook the present study to determine whether there is a direct effect of sera from patients with renal insufficiency on mesangial cell proliferation. Growth-arrested mesangial cells were incubated with sera from patients with varying degrees of renal insufficiency and then pulsed with [3H]thymidine. Thymidine uptake was progressively greater with more advanced renal failure and was linearly correlated with (creatinine)-1. This data suggests that serum from patients with loss of renal function contains mitogens capable of directly enhancing mesangial cell proliferation.
Mattana
J
J
Department of Medicine, Long Island Jewish Medical Center, New Hyde Park, NY 11042.
Abramovici
M
M
Singhal
P C
PC
D000818:Animals; D002455:Cell Division / Q000187:drug effects*; D002478:Cells, Cultured; D003404:Creatinine / Q000008:administration & dosage / Q000097:blood; D004305:Dose-Response Relationship, Drug; D005920:Glomerular Mesangium / Q000166:cytology* / Q000378:metabolism*; D006801:Humans; D051381:Rats; D017207:Rats, Sprague-Dawley; D051437:Renal Insufficiency / Q000097:blood*; D013936:Thymidine / Q000008:administration & dosage / Q000378:metabolism; D014508:Urea / Q000008:administration & dosage / Q000097:blood*
D014508:Urea; D003404:Creatinine; D013936:Thymidine