Molecular cloning and functional expression of the human gallbladder cholecystokinin A receptor.
Through binding to cholecystokinin (CCK) A receptors, CCK is an important physiologic regulator of both gallbladder contraction and pancreatic enzyme secretion. In this work, we have used a combination of hybridization screening of a cDNA library and polymerase chain reaction to clone a 2.1 kb cDNA which encodes the human gallbladder CCKA receptor. Nucleotide sequence analysis revealed an open reading frame encoding a 428 amino acid protein, with seven putative transmembrane domains and a high degree of homology with the rat CCKA receptor. COS cells transfected with this cDNA clone bound CCK-8 and L-364,718 with high affinities appropriate for the CCKA receptor, and exhibited a transient increase in intracellular calcium in response to CCK. This should provide an important resource for the analysis of the role of this receptor in human physiology and pathophysiology.
Ulrich
C D
CD
Center for Basic Research in Digestive Diseases, Mayo Clinic, Rochester, MN 55905.
Ferber
I
I
Holicky
E
E
Hadac
E
E
Buell
G
G
Miller
L J
LJ
D000595:Amino Acid Sequence; D000818:Animals; D001667:Binding, Competitive; D002478:Cells, Cultured; D003001:Cloning, Molecular; D004247:DNA; D005704:Gallbladder / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D011949:Receptors, Cholecystokinin / Q000235:genetics* / Q000378:metabolism; D015290:Second Messenger Systems; D017386:Sequence Homology, Amino Acid; D014162:Transfection
D011949:Receptors, Cholecystokinin; D004247:DNA