A rapid and simple method for determining the DNA sequences of fragments inserted into vaccinia virus.
Recombinant virus vectors such as vaccinia virus, adenovirus and herpesvirus are frequently used to express a variety of foreign products. A rapid method allowing the precise identification of recombinants would be useful to confirm the nature of a newly produced recombinant and, in particular, to discriminate between recombinants bearing near-identical foreign products. Using vaccinia virus, we describe a method that in one day provides sequence analysis of the recombinant viral DNA.
Sheng
N
N
Dept. of Periodontics, University of Michigan, Ann Arbor 48109.
Zhang
J
J
Whitton
J L
JL
McKee
T
T
D001483:Base Sequence; D001709:Biotechnology; D015342:DNA Probes; D004274:DNA, Recombinant / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics*; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA / Q000379:methods*; D014616:Vaccinia virus / Q000235:genetics*
D015342:DNA Probes; D004274:DNA, Recombinant; D004279:DNA, Viral