Role of glutathione in the maturation and fertilization of pig oocytes in vitro.
The present study examined, by treatment of buthionine sulfoximine (BSO), which is a specific inhibitor of glutathione (GSH) synthesis, the role of GSH in the maturation and fertilization of pig oocytes in vitro. Follicular oocytes collected from prepubertal gilts at a local slaughterhouse were cultured for 36 h in Waymouth MB 752/1 with or without BSO (1 mM), fertilized in vitro, and assessed for GSH concentration (before insemination), maturation, and fertilization. The addition of BSO to maturation medium immediately after culture (Group I), 12 h after culture (Group II), or 24 h after culture (Group III) significantly decreased the GSH concentration in pig oocytes compared with the control (P < 0.01), whereas the rate of cumulus mass expansion at 36 h of culture and the rates of nuclear maturation and sperm penetration following in vitro insemination did not differ. However, the rate of pig oocytes having condensed sperm heads was significantly lower and the rate of male pronucleus formation of pig oocytes was significantly higher in oocytes matured in the control and Group III than in oocytes matured in Groups I and II (P < 0.01). In experiment 2, when BSO was added to maturation media 15, 18, 21, or 24 h after culture, the rate of pig oocytes having condensed sperm heads was significantly lower and the rate of male pronucleus formation of pig oocytes was significantly higher in oocytes matured in the medium supplemented with BSO at 21 or 24 h of culture than in oocytes matured in other groups (P < 0.05 or P < 0.01).(ABSTRACT TRUNCATED AT 250 WORDS)
Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University, Japan.
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Yoshida
D000818:Animals; D019328:Buthionine Sulfoximine; D003593:Cytoplasm / Q000378:metabolism; D005260:Female; D005307:Fertilization in Vitro; D005978:Glutathione / Q000096:biosynthesis*; D066298:In Vitro Techniques; D008297:Male; D008717:Methionine Sulfoximine / Q000031:analogs & derivatives / Q000494:pharmacology; D009865:Oocytes / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D013084:Sperm-Ovum Interactions / Q000502:physiology; D013552:Swine
D008717:Methionine Sulfoximine; D019328:Buthionine Sulfoximine; D005978:Glutathione