Cytogenetic abnormalities in colon cancer patients: a comparison of T- and B-lymphocytes.
Cytogenetic analysis of peripheral blood lymphocytes of 19 colorectal cancer patients was carried out in short term blood cultures (T-cells) as well as in Epstein-Barr virus transformed B-cell lymphoblastoid cell lines. One hundred Giemsa-banded metaphases from the T lymphocytes and 50 metaphases from the B lymphocytes of each patient were evaluated for cytogenetic abnormalities. Clonality was not observed in every paired sample. Structural and/or numerical aberrations were most frequent in chromosomes #1, #2, #5, #7, #9, #12, #14, #17, #18 and #21. Aberrations among these chromosomes could be observed individually in either of the cultures, which proves that the analysis of both cultures (T and B cells) is complementary to each other. In some cases involving multiple primary cancers it was interesting that the specific chromosomal change, crucial for a particular malignancy, was identified only in the lymphoblastoid cell line analysis. This supports the notion that B-cell analysis can serve as a useful adjunct to the study of short-term blood cultures and also poses a question as to whether the specific chromosomal changes observed in the analysis are confined to the B-cell lineage.
Department of Cell Biology, University of Texas M.D. Anderson Cancer Center, Houston 77030.
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Dave
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Hopwood
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Hughes
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Jackson
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Melillo
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Pathak
D000230:Adenocarcinoma / Q000097:blood / Q000235:genetics*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001402:B-Lymphocytes / Q000502:physiology*; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D002872:Chromosome Deletion; D003110:Colonic Neoplasms / Q000097:blood / Q000235:genetics*; D015179:Colorectal Neoplasms / Q000097:blood / Q000235:genetics; D005260:Female; D006801:Humans; D007621:Karyotyping; D008297:Male; D008875:Middle Aged; D013601:T-Lymphocytes / Q000502:physiology*