In vitro granuloma formation by spleen cells treated with psoralen plus long-wave ultraviolet radiation.
In vitro granulomas are induced by culturing murine spleen cells with artificial microparticles, dextran beads. In the presence of 0.5 microgram/mL 8-methoxypsoralen, UVA radiation (0.2-2.0 J/cm2) suppressed granuloma formation in a UVA dose-dependent manner. The doses of PUVA did not affect the cell viability as assessed by trypan blue exclusion. The time course of granuloma formation in 0.5 J/cm2 PUVA-treated cells was similar to that of normal spleen cells, with a maximum granuloma index at day 3 of culture, although a 49-63% suppression of granuloma formation was observed. PUVA-treated, nonadherent cells produced the same granuloma index when cultured with normal adherent cells. In contrast, a smaller granuloma index was observed in PUVA-treated adherent cells even when they were cultured with normal nonadherent cells. These data suggest that PUVA alters macrophages, resulting in the suppression of granuloma formation in vitro.
Department of Dermatology, Faculty of Medicine, Kyoto University, Japan.
H
H
Okamoto
Z
Z
Guo
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S
Imamura
D000818:Animals; D002448:Cell Adhesion; D002478:Cells, Cultured; D005260:Female; D006099:Granuloma / Q000209:etiology* / Q000473:pathology; D008730:Methoxsalen / Q000633:toxicity*; D051379:Mice; D008809:Mice, Inbred C3H; D011701:PUVA Therapy; D011833:Radiation Injuries, Experimental; D013154:Spleen / Q000187:drug effects* / Q000473:pathology / Q000528:radiation effects*; D013158:Splenic Diseases / Q000209:etiology / Q000473:pathology; D014466:Ultraviolet Rays
D008730:Methoxsalen