Synthesis and secretion of vascular permeability factor/vascular endothelial growth factor by human retinal pigment epithelial cells.
The presence of the secreted angiogenic molecule, vascular permeability factor/vascular endothelial growth factor, was investigated in human retinal pigment epithelial cells. Northern and in situ hybridization analysis of cultured cells identified vascular permeability factor/vascular endothelial growth factor mRNA. Western analysis of cell lysates identified a 42 kD protein that comigrated with human recombinant vascular permeability factor/vascular endothelial growth factor. Immunoassay measurements detected vascular permeability factor/vascular endothelial growth factor protein in cell lysates and conditioned media in vitro and in cell lysates isolated directly from post-mortem eyes. These data demonstrate that human retinal pigment epithelial cells can synthesize the secreted angiogenic peptide vascular permeability factor/vascular endothelial growth factor in vitro and in situ. The production and secretion of this factor by human retinal pigment epithelial cells may be important in the pathogenesis of ocular neovascularization.
Department of Surgery, Childrens Hospital, Boston, Massachusetts 02115.
A P
AP
Adamis
D T
DT
Shima
K T
KT
Yeo
T K
TK
Yeo
L F
LF
Brown
B
B
Berse
P A
PA
D'Amore
J
J
Folkman
D016375:Antisense Elements (Genetics); D015152:Blotting, Northern; D015153:Blotting, Western; D002478:Cells, Cultured; D004591:Electrophoresis, Polyacrylamide Gel; D016228:Endothelial Growth Factors / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D006801:Humans; D017403:In Situ Hybridization; D008222:Lymphokines / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism*; D010857:Pigment Epithelium of Eye / Q000378:metabolism*; D015347:RNA Probes; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors
D016375:Antisense Elements (Genetics); D016228:Endothelial Growth Factors; D008222:Lymphokines; D015347:RNA Probes; D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors