A siderophore production mutant of Bordetella bronchiseptica cannot use lactoferrin as an iron source.
Bordetella bronchiseptica secreted a hydroxamate siderophore when grown in Fe-depleted medium. A Tn5lac insertion mutant of B. bronchiseptica, DBB22, did not produce this hydroxamate siderophore and was incapable of using lactoferrin as an Fe source. Our data suggest that B. bronchiseptica uses a siderophore for removal of Fe from lactoferrin and transferrin rather than relying upon a receptor for these host Fe-binding proteins.
Foster
L A
LA
Department of Microbiology, School of Medicine and Biomedical Sciences, State University of New York, Buffalo 14214.
Dyer
D W
DW
D000818:Animals; D015139:Blotting, Southern; D016950:Bordetella bronchiseptica / Q000254:growth & development / Q000378:metabolism*; D002417:Cattle; D006801:Humans; D007501:Iron / Q000378:metabolism*; D007781:Lactoferrin / Q000378:metabolism*; D009154:Mutation; D017262:Siderophores / Q000096:biosynthesis* / Q000235:genetics; D014168:Transferrin / Q000378:metabolism
D017262:Siderophores; D014168:Transferrin; D007501:Iron; D007781:Lactoferrin