Direct polymerase chain reaction for detection of human immunodeficiency virus in blood spot residues on filter paper after elution of antibodies: an adjunct to serological surveys for estimating vertical transmission rates among human immunodeficiency virus antibody-positive newborns.
Blood spot residues on filter paper circles from which antibodies have been eluted remain suitable for follow-up polymerase chain reaction to detect human immunodeficiency virus (HIV) provirus. A method has been developed for specimen processing and nested polymerase chain reaction amplification with a single tube. HIV-positive specimens can be detected by simple, direct electrophoresis of amplified material to a single copy of provirus. The procedure is particularly suited for survey purposes to estimate rates of vertical transmission of virus among HIV antibody-positive newborns, in whom virus loads may be low.
Virology Laboratory, New York State Department of Health, Wadsworth Center for Laboratories and Research, Albany 12201-0509.
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D004279:DNA, Viral / Q000097:blood; D005069:Evaluation Studies as Topic; D005260:Female; D006678:HIV / Q000235:genetics* / Q000302:isolation & purification*; D015483:HIV Antibodies / Q000097:blood; D015658:HIV Infections / Q000276:immunology / Q000382:microbiology / Q000635:transmission*; D006801:Humans; D007231:Infant, Newborn; D008431:Maternal-Fetal Exchange; D016133:Polymerase Chain Reaction / Q000379:methods*; D011247:Pregnancy; D011533:Proviruses / Q000235:genetics / Q000302:isolation & purification; D014766:Viremia / Q000382:microbiology
D004279:DNA, Viral; D015483:HIV Antibodies