A novel human tyrosine kinase gene inducible in T cells by interleukin 2.
We have cloned a novel human protein tyrosine kinase gene specific to T cells by the polymerase chain reaction method. This gene encodes a 620 amino-acid polypeptide including a catalytic domain for tyrosine kinase, an SH2 domain and an SH3 domain, seemingly belonging to the src family. However, characteristics of a long unique N-terminal stretch and lack of a myristylation site at the N-terminus and of a kinase regulatory tyrosine residue in the C-terminus classify this molecule into a new subfamily comprising recently cloned mouse tec, itk/tsk and human atk/bpk genes. This gene was transcriptionally induced in normal T cells by interleukin 2 stimulation. These results suggest the crucial roles of this gene in T cell proliferation and differentiation.
Department of Microbiology, Tohoku University School of Medicine, Sendai, Japan.
N
N
Tanaka
H
H
Asao
K
K
Ohtani
M
M
Nakamura
K
K
Sugamura
D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D004790:Enzyme Induction / Q000187:drug effects; D016391:Genes, src; D015368:Human T-lymphotropic virus 1 / Q000235:genetics; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology*; D008213:Lymphocyte Activation; D051379:Mice; D008969:Molecular Sequence Data; D005810:Multigene Family; D011505:Protein-Tyrosine Kinases / Q000096:biosynthesis / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013601:T-Lymphocytes / Q000187:drug effects / Q000201:enzymology* / Q000276:immunology; D014407:Tumor Cells, Cultured
D007376:Interleukin-2; D012333:RNA, Messenger; D004247:DNA; D011505:Protein-Tyrosine Kinases