Chimeric cDNA studies of Theiler's murine encephalomyelitis virus neurovirulence.
Strain GDVII and other members of the GDVII subgroup of Theiler's murine encephalomyelitis virus are highly neurovirulent and rapidly fatal, while strain DA and other members of the TO subgroup produce a chronic, demyelinating disease. GDVII/DA chimeric cDNA studies suggest that a major neurovirulence determinant is within the GDVII 1B through 1D capsid protein coding region, although the additional presence of upstream GDVII sequences, including the 5' untranslated region, contributes to full neurovirulence. Our studies indicate that there are limitations in precisely delineating neurovirulence determinants with chimeric cDNAs between evolutionarily diverged viruses, such as GDVII and DA.
Department of Neurology/MC2030, University of Chicago Medical Center, Illinois 60637.
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Senkowski
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D000818:Animals; D002460:Cell Line; D002678:Chimera; D006224:Cricetinae; D004247:DNA / Q000235:genetics; D004274:DNA, Recombinant; D007739:L Cells; D004680:Maus Elberfeld virus / Q000235:genetics / Q000472:pathogenicity*; D051379:Mice; D008815:Mice, Inbred Strains; D009422:Nervous System Diseases / Q000382:microbiology*; D012367:RNA, Viral / Q000235:genetics; D014779:Virus Replication
D004274:DNA, Recombinant; D012367:RNA, Viral; D004247:DNA