Quick assay of serum HBs antibody levels using latex agglutination-integrating sphere turbidimetric assay (ISTA).
A method for determining serum HBs antibody applying the principle of integrating sphere turbidimetric assay (ISTA) by latex agglutination was developed. The minimum detectable level of HBs antibody by this method is 12.5 IU/L, indicating that this method is 3 times or more sensitive with better reproducibility and specificity than the passive hemagglutination (PHA) method. With a cut-off level of 25 IU/L, the possible highest simultaneous reading by this method was 1,000 IU/L. Serum HBs antibody can be readily measured in 10 or so minutes by this method if a fully automated EL-1000 analyzer is used. This rapid and simple method for determining serum HBs antibody will be useful not only clinically, but also in preventive medicine.
Department of Laboratory Medicine, Mie University School of Medicine, Tokyo, Japan.
M
M
Yamashiki
Y
Y
Kosaka
A
A
Nishimura
I
I
Kondo
A
A
Tomita
H
H
Kishioka
D004797:Enzyme-Linked Immunosorbent Assay; D006510:Hepatitis B Antibodies / Q000097:blood*; D017325:Hepatitis B Vaccines / Q000276:immunology; D006801:Humans; D007841:Latex Fixation Tests; D009391:Nephelometry and Turbidimetry; D015203:Reproducibility of Results; D014611:Vaccination
D006510:Hepatitis B Antibodies; D017325:Hepatitis B Vaccines