[Induction of gene (mRNA) expression of CD23, CD25 and CD4 on eosinophils by eosinophilopoietic growth lymphokines].
The effects of IL-3, GM-CSF and IL-5 on the expression of CD23 (Fc(E)RII), CD25 (IL-2R/p55) and CD4 on an eosinophilic cell line (EoL-3) were investigated by flow cytometry. A separate incubation with IL-3, GM-CSF or IL-5, did not induce the expression of CD23, CD25, or CD4. However, a sequential incubation with IL-3, then with IL-3 and GM-CSF, induced a significant expression of CD23 and CD25. After a further incubation with IL-3, GM-CSF and IL-5, CD4 was then expressed, while CD23 and CD25 expression still increased. The kinetics of expression of CD11b were parallel to that of CD23, but the expression of the transferrin receptor (CD71) remained negative. Northern blot analysis revealed the presence of mRNA encoding CD23, CD25 and CD4 in EoL-3 stimulated by IL-3, GM-CSF and IL-5. Culture with GM-CSF induced the binding of radiolabeled IL-5 to EoL-3 cells, with an increased affinity after a sequential incubation with IL-3, GM-CSF and IL-5. These data indicate that IL-3, GM-CSF and IL-5, might be involved in the expression of functional markers on eosinophil membrane.
Fourth Department of Internal Medicine, Kinki University School of Medicine.
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J
Chihara
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S
Nakajima
D015704:CD4 Antigens / Q000235:genetics*; D002455:Cell Division; D002478:Cells, Cultured; D016207:Cytokines / Q000502:physiology*; D004804:Eosinophils / Q000166:cytology / Q000276:immunology*; D015870:Gene Expression; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000502:physiology; D006801:Humans; D007377:Interleukin-3 / Q000502:physiology; D015848:Interleukin-5 / Q000502:physiology; D012333:RNA, Messenger / Q000032:analysis*; D017455:Receptors, IgE / Q000235:genetics*; D015375:Receptors, Interleukin-2 / Q000235:genetics*
D015704:CD4 Antigens; D016207:Cytokines; D007377:Interleukin-3; D015848:Interleukin-5; D012333:RNA, Messenger; D017455:Receptors, IgE; D015375:Receptors, Interleukin-2; D016178:Granulocyte-Macrophage Colony-Stimulating Factor