[Insertion mutants of the vaccinia virus. The effect of inactivating E7R and D8L genes on the biological properties of the virus].
The integrative plasmids with the expressive marker gene for beta-galactosidase were constructed for insertional inactivation of nonessential genes E7R and D8L of vaccinia virus located in the central region of the viral genome. Inactivation of the D8L gene in the strains WR and LIVP results in smaller viral plaques in the culture of chicken embryo cells, decreases the viral ability to propagate in mouse brain and has no effect on the size and character of damage in intracutaneous infection of rabbits. Inactivation of E7R gene did not affect the known biological properties of the virus. The existence of the nonessential genes in the central region of the viral genome, inactivation of which does not result in viral attenuation, can be used for increase of antigenic activity of the live attenuated vaccines.
Chernos
V I
VI
Vovk
T S
TS
Ivanova
O N
ON
Antonova
T P
TP
Loparev
V N
VN
D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D051379:Mice; D016254:Mutagenesis, Insertional; D010957:Plasmids; D011817:Rabbits; D011995:Recombination, Genetic; D014616:Vaccinia virus / Q000235:genetics* / Q000472:pathogenicity / Q000502:physiology; D014774:Virulence / Q000235:genetics; D014779:Virus Replication / Q000235:genetics; D001616:beta-Galactosidase / Q000235:genetics
D001616:beta-Galactosidase
Insertsionnye mutanty virusa ospovaktsiny. Vliianie inaktivatsii genov E7R i D8L na biologicheskie svoĭstva virusa.