Cadmium-induced arteriolar constriction in skeletal muscle microcirculation.
Cadmium, an environmental pollutant, is known to induce hypertension in animal models, in part via an increase in peripheral vascular resistance. Since prior studies have investigated the vascular effects of cadmium using large, nonresistance arteries, we directly assessed cadmium's action on resistance size arterioles in skeletal muscle using the intact rat cremaster muscle preparation. Cadmium evoked a concentration-dependent constriction of the large arterioles (120 to 50 microns in diameter) but elicited no change in the diameter of smaller arterioles (30 to 15 microns). Blockade of alpha-adrenergic receptors did not diminish the constrictor response of the larger arterioles to cadmium, but bathing the cremaster muscles with a solution containing low calcium attenuated the arteriolar constriction to cadmium. Calcium repletion caused the arterioles to constrict further. These observations provide the first direct evidence that cadmium constricts resistance arterioles in skeletal muscle. The cadmium constriction: (1) is selective for the large arterioles, (2) is not mediated by alpha-adrenergic receptors, and (3) is influenced by the extracellular level of calcium. We conclude that arteriolar constriction in skeletal muscle tissue may play a role in the hypertensive actions of cadmium.
Department of Pharmacology and Toxicology, University of Louisville, Kentucky 40292.
C
C
Zhang
G S
GS
Thind
I G
IG
Joshua
J T
JT
Fleming
D000818:Animals; D001160:Arterioles / Q000187:drug effects; D002104:Cadmium / Q000494:pharmacology*; D002118:Calcium / Q000494:pharmacology; D008297:Male; D008833:Microcirculation; D009132:Muscles / Q000098:blood supply*; D051381:Rats; D011921:Rats, Inbred WKY; D013565:Sympatholytics / Q000494:pharmacology; D014661:Vasoconstriction / Q000187:drug effects*
D013565:Sympatholytics; D002104:Cadmium; D002118:Calcium