Determination of oestrogen responsiveness of breast cancer by competitive reverse transcription-polymerase chain reaction.
Competitive polymerase chain reaction assays have been developed for the quantitation of oestrogen receptor mRNA and two oestrogen-regulated mRNAs (progesterone receptor and pNR-2/pS2) in breast cancer cells. These assays are more sensitive than traditional hybridisation techniques, do not require the use of radioisotopes, measure absolute amounts of messenger RNAs and can be used to measure the expression of mRNAs in small numbers of tumour cells obtained by fine-needle aspiration (FNA). These assays should prove useful for predicting the hormone responsiveness of breast cancer from tumour cells obtained by FNA at diagnosis and could be particularly useful in the management of elderly/frail patients who receive primary tamoxifen, or in other patients for whom tumour tissue for standard biochemical measurements is not available.
Department of Pathology, Royal Victoria Infirmary, Newcastle upon Tyne, UK.
M
M
Carr
F E
FE
May
T W
TW
Lennard
B R
BR
Westley
D001483:Base Sequence; D001707:Biopsy, Needle; D001943:Breast Neoplasms / Q000235:genetics / Q000473:pathology / Q000648:ultrastructure*; D004967:Estrogens; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation; D009363:Neoplasm Proteins / Q000032:analysis / Q000235:genetics; D009376:Neoplasms, Hormone-Dependent / Q000235:genetics / Q000473:pathology / Q000648:ultrastructure*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011506:Proteins; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D012334:RNA, Neoplasm / Q000235:genetics / Q000378:metabolism; D011960:Receptors, Estrogen / Q000032:analysis*; D011980:Receptors, Progesterone / Q000032:analysis / Q000235:genetics; D014158:Transcription, Genetic; D000071163:Trefoil Factor-1; D014407:Tumor Cells, Cultured; D025521:Tumor Suppressor Proteins
D004967:Estrogens; D009363:Neoplasm Proteins; D011506:Proteins; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone; C051838:TFF1 protein, human; D000071163:Trefoil Factor-1; D025521:Tumor Suppressor Proteins