Purification and properties of endo-beta-1,4-D-galactanase from Aspergillus niger.
An endo-beta-1,4-D-galactanase was purified 1735-fold from a commercial enzyme preparation of Aspergillus niger. The endogalactanase was homogeneous by SDS-PAGE, having an apparent molecular weight of 32,000. It specifically hydrolyzed beta-1,4-D-galactan, and is shown to be capable of releasing galactosyl oligomers and galactose from the soybean pectic polysaccharides.
Food R & D Center, Japan Tobacco Inc., Kanagawa, Japan.
F
F
Yamaguchi
S
S
Inoue
C
C
Hatanaka
D001234:Aspergillus niger / Q000201:enzymology*; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D006026:Glycoside Hydrolases; D006863:Hydrogen-Ion Concentration; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism; D013379:Substrate Specificity; D013696:Temperature; D001616:beta-Galactosidase / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*
D011134:Polysaccharides; D006026:Glycoside Hydrolases; D001616:beta-Galactosidase; C094192:arabinogalactan endo-1,4-beta-galactosidase