Polymerase chain reaction-based detection of hepatitis D virus RNA in patients infected with human immunodeficiency virus.
We used the polymerase chain reaction (PCR) to detect hepatitis D (HD) viremia in patients infected with the human immunodeficiency virus (HIV). Nineteen (9%) of 206 such patients were prospectively found to be infected by HDV. Thirty-one anti-HIV-positive patients were studied by means of PCR and the results were analysed according to HDV and hepatitis B virus (HBV) serological status. HDV-PCR was positive in five patients. Two had detectable serum HDV antigen. Four patients had anti-HD IgM and IgG antibodies. All these patients were HBs antigen-positive, and three were HBV-DNA positive. All the other patients were HDV-PCR-negative. Statistical analysis suggested more extensive liver damage and immunological impairement in HDV-PCR positive patients. In this unselected HIV-infected population, HDV-RNA detection by PCR was only evidenced in HDV infected patients in whom 5/19 were positive. This test allowed direct diagnosis of HDV viremia and will be useful for the monitoring of HDV infection.
CHU Avicenne, Paris, France.
C
C
Lecot
V
V
Jeantils
L
L
Ovaguimian
A
A
Krivitzky
C
C
Bréchot
P
P
Dény
D017088:AIDS-Related Opportunistic Infections / Q000150:complications / Q000175:diagnosis; D000956:Antigens, Viral / Q000097:blood; D001483:Base Sequence; D015658:HIV Infections / Q000150:complications / Q000382:microbiology*; D006508:Hepatitis Antibodies / Q000097:blood; D003699:Hepatitis D / Q000150:complications / Q000175:diagnosis; D003698:Hepatitis Delta Virus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D035923:Hepatitis delta Antigens; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000097:blood* / Q000235:genetics; D014766:Viremia / Q000175:diagnosis
D000956:Antigens, Viral; D006508:Hepatitis Antibodies; D035923:Hepatitis delta Antigens; D012367:RNA, Viral; C442244:hepatitis delta virus large antigen