HT29-18-C1 intestinal cells: a new model for studying the epithelial transport of drugs.
A polarized differentiated subclone of HT29, a human colon carcinoma cell line, was used to measure the passage of drugs across the intestinal epithelium. These cells, HT29-18-C1, when grown on permeable filters, formed tight monolayers of high electrical resistance (> 400 omega cm2). Electron micrographs revealed the presence of numerous apical microvilli and well developed junctional complexes. The transepithelial passage of various drugs was studied: the transepithelial permeability coefficients ranged from 0.8 x 10(-6) cm/s for fluorescein, to 4.8 x 10(-5) cm/s for the most lipophilic molecule, testosterone. For the five compounds tested, there was a coarse correlation between the apparent transepithelial permeability coefficient and the octanol/buffer distribution coefficient. The HT29-18-C1 subclone thus represents a new in-vitro model for studying the intestinal absorption of drugs.
Wils
P
P
UMR 133 CNRS/RPR, Centre de Recherche de Vitry-Alfortville, Vitry-sur-Seine, France.
Legrain
S
S
Frenois
E
E
Scherman
D
D
D020003:1-Octanol; D001692:Biological Transport; D002999:Clone Cells / Q000378:metabolism / Q000648:ultrastructure; D004848:Epithelium / Q000378:metabolism; D019793:Fluorescein; D005452:Fluoresceins / Q000378:metabolism; D006801:Humans; D007408:Intestinal Absorption; D008954:Models, Biological; D000442:Octanols / Q000378:metabolism; D010539:Permeability; D004364:Pharmaceutical Preparations / Q000378:metabolism*; D013739:Testosterone / Q000378:metabolism; D014407:Tumor Cells, Cultured / Q000378:metabolism / Q000648:ultrastructure
D005452:Fluoresceins; D000442:Octanols; D004364:Pharmaceutical Preparations; D013739:Testosterone; D020003:1-Octanol; D019793:Fluorescein