Contribution of gastrin to cysteamine-induced gastric acid secretion in rats.
The role of circulating gastrin in cysteamine induced gastric acid secretion was examined in conscious male Wistar rats, provided with a portal vein catheter, a jugular vein catheter and a pyloric drainage tube. Intravenous infusion of 0.3 nmol/kg.30 min of gastrin 17-l resulted in serum gastrin concentrations of 1138 +/- 151 pg/ml and gastric acid secretion of 104 +/- 36 mumol H+/kg.30 min. This acid response was abolished by intravenous injection of 60 microliters of a gastrin-antiserum, indicating the efficacy of immunoneutralization with this antiserum in vivo. Intravenous bolus administration of 125 mg/kg of cysteamine induced increases in serum gastrin concentration (864 +/- 96 pg/ml) and gastric acid outputs (107 +/- 27 mumol H+/kg.30 min) not significantly different from the gastrin 17-l infusion experiments. Gastrin antiserum abolished cysteamine-induced gastric acid secretion, indicating that gastric acid secretion induced by 125 mg/kg of cysteamine is largely mediated by circulating gastrin in rats.
Department of Gastroenterology, University Hospital of Leiden, The Netherlands.
F J
FJ
van de Brug
J B
JB
Jansen
I J
IJ
Kuijpers
C B
CB
Lamers
D000818:Animals; D001665:Binding Sites / Q000187:drug effects; D003543:Cysteamine / Q000494:pharmacology*; D005744:Gastric Acid / Q000378:metabolism*; D005755:Gastrins / Q000008:administration & dosage / Q000097:blood / Q000502:physiology*; D007106:Immune Sera / Q000008:administration & dosage; D007262:Infusions, Intravenous; D008297:Male; D051381:Rats; D017208:Rats, Wistar
D005755:Gastrins; D007106:Immune Sera; D003543:Cysteamine