[Cloning and expression of determinants of Bacillus anthracis protective antigen in Escherichia coli, Bacillus subtilis, and Bacillus anthracis cells].
A determinant for a protective antigen (pag) of Bacillus anthracis STI has been cloned. Its expression in Escherichia coli, Bacillus subtilis and Bacillus anthracis cells has been studied. The hybrid plasmids were obtained carrying the different fragments of the gene. The plasmids pPA2 and pPA3 having the 3'-end fragment of pag deleted (the size of 1 kb) still code for a part of protective antigen preserving the immunological and protective properties.
V M
VM
Tedikov
A P
AP
Dobritsa
D000881:Anthrax / Q000276:immunology; D000942:Antigens, Bacterial / Q000235:genetics*; D001408:Bacillus anthracis / Q000235:genetics / Q000276:immunology*; D001412:Bacillus subtilis / Q000235:genetics*; D003001:Cloning, Molecular; D004926:Escherichia coli; D010957:Plasmids
D000942:Antigens, Bacterial
Klonirovanie i ékspressiia determinanty protektivnogo antigena Bacillus anthracis v kletkakh Escherichia coli, Bacillus subtilis i Bacillus anthracis.