In vitro activity of FK037, a new parenteral cephalosporin, against anaerobic bacteria.
The activity of FK037, a new parenteral cephalosporin, was compared with those of cefpirome, ceftazidime, and flomoxef against 322 recent clinical isolates of anaerobic bacteria. A fastidious facultative anaerobe, Gardnerella vaginalis, was also studied. FK037 inhibited 90% of isolates of Peptostreptococcus anaerobius, Peptostreptococcus asaccharolyticus, Clostridium perfringens, Mobiluncus spp., G. vaginalis, and Porphyromonas gingivalis at < or = 0.78 micrograms/ml. The MICs of FK037 for 50 and 90% of Bacteroides fragilis isolates were 25 and > 200 micrograms/ml, respectively; the activity of FK037 was comparable to those of cefpirome and ceftazidime but lower than that of flomoxef. The activity of FK037 against Fusobacterium nucleatum, Fusobacterium varium, and Bilophila wadsworthia decreased when inoculum size was increased from 10(6) to 10(8) CFU/ml. Little influence of inoculum size on the activity of FK037 was observed for other isolates tested. Medium pH affected the activity of FK037 against F. varium (MICs at pHs 5 and 7, 3.13 and 100 micrograms/ml, respectively) and Bacteroides gracilis (MICs at pHs 5 and 7, 12.5 and 1.56 micrograms/ml, respectively) but not against other organisms tested. FK037 was less resistant than flomoxef to hydrolysis by beta-lactamase group 2e derived from B. fragilis GAI 0558 and GAI 10150.
Institute of Anaerobic Bacteriology, Gifu University School of Medicine, Japan.
N
N
Kato
H
H
Kato
Y
Y
Tanaka
K
K
Bando
K
K
Watanabe
K
K
Ueno
D001421:Bacteria, Anaerobic / Q000187:drug effects* / Q000254:growth & development / Q000302:isolation & purification; D001424:Bacterial Infections / Q000188:drug therapy / Q000382:microbiology; D015296:Ceftizoxime / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D002511:Cephalosporins / Q000494:pharmacology*; D015169:Colony Count, Microbial; D003470:Culture Media; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007700:Kinetics; D008826:Microbial Sensitivity Tests; D001618:beta-Lactamases / Q000378:metabolism
D002511:Cephalosporins; D003470:Culture Media; C079769:cefoselis; D015296:Ceftizoxime; D001618:beta-Lactamases