Turning DNA replication on and off.
DNA replication is coupled to cell cycle progression at a major regulatory point in the G1 phase of the cell cycle. At this point, the catalytic subunit of a protein kinase (encoded by the CDC28 gene in budding yeast or the homologous CDC2 gene in other eukaryotes) is activated by binding to a positively acting regulatory subunit, a cyclin. Recent research has revealed evidence for two pathways that might connect these kinases to the proteins that replicate DNA: activation of an essential replication factor, or removal of the block that limits genome duplication to once per cell cycle.
Roberts
J M
JM
Department of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington 98104.
D002453:Cell Cycle; D016213:Cyclins; D004261:DNA Replication / Q000502:physiology*; D005057:Eukaryotic Cells; D011494:Protein Kinases / Q000502:physiology*
D016213:Cyclins; D011494:Protein Kinases