Determination of colchicine in biological fluids by reverse-phase HPLC. Variation of colchicine levels in rats.
A reverse-phase HPLC method for the determination of colchicine in biological fluids is proposed. Blood, liver and kidney colchicine concentrations were determined in rats at different times following intraperitoneal (i.p.) injection of 10 mg/kg of the drug. Colchicine was extracted from the samples studied using dichloromethane at pH 8. The dry extract was redissolved in the mobile phase and analyzed with simultaneous detection at 254 and 350 nm.
Fernández
P
P
Department of Legal Medicine, Faculty of Medicine, University of Santiago de Compostela, Spain.
Bermejo
A M
AM
Tabernero
M J
MJ
López-Rivadulla
M
M
Cruz
A
A
D000818:Animals; D001826:Body Fluids / Q000737:chemistry*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003078:Colchicine / Q000032:analysis*; D005554:Forensic Medicine / Q000379:methods*; D007274:Injections, Intraperitoneal; D007668:Kidney / Q000737:chemistry; D008099:Liver / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D003078:Colchicine