Synthetic carotenoids as internal standards for plasma micronutrient analyses by high-performance liquid chromatography.
A method is described for the synthesis of new carotenoids by transesterification of ethyl-beta-apo-8'-carotenoate. The rate of transesterification was temperature-dependent with highest yields using primary alcohols. Reaction conditions were found avoiding Z isomerization. The structures of the new products were confirmed by ultraviolet, mass, 1H and 13C nuclear magnetic resonance spectroscopy. The synthetic carotenoids were used as internal standards for reversed-phase high-performance liquid chromatographic (HPLC) analyses of lipid phase plasma micronutrients. Retention times for the trans-esterified products increased as a function of alcohol chain length allowing a choice of synthetic carotenoids for use as internal standards for carotenoid quantitation without interference with the detection of analytes during HPLC analysis.
Cancer Research Center of Hawaii, Honolulu 96813.
A A
AA
Franke
L J
LJ
Custer
R V
RV
Cooney
D000328:Adult; D002338:Carotenoids / Q000097:blood* / Q000138:chemical synthesis; D002851:Chromatography, High Pressure Liquid; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008875:Middle Aged; D015394:Molecular Structure; D012015:Reference Standards; D013056:Spectrophotometry, Ultraviolet; D014801:Vitamin A / Q000097:blood*; D014810:Vitamin E / Q000097:blood*
D014801:Vitamin A; D014810:Vitamin E; D002338:Carotenoids