Cloning of the mouse hsp25 gene and an extremely conserved hsp25 pseudogene.
A genomic clone of the murine gene encoding the small heat-shock protein, Hsp25, was isolated. The coding region is interrupted by two introns of 128 bp and approximately 600 bp at identical positions as the human hsp27 gene. The 5' flanking regions of the mouse and human genes are very strongly conserved and contain several sequence motives for the transcription factors, HSF and Sp1. In the same screen we also isolated a hsp25 pseudogene. The sequence conservation between this pseudogene and hsp25 cDNA is very high (99%) indicating that this pseudogene emerged very recently.
Department of Pathology, University Hospital, Zürich, Switzerland.
E
E
Fröhli
A
A
Aoyama
R
R
Klemenz
D000595:Amino Acid Sequence; D000818:Animals; D010582:Bacteriophage lambda / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular; D017124:Conserved Sequence; D004268:DNA-Binding Proteins / Q000235:genetics; D000076249:Heat Shock Transcription Factors; D006360:Heat-Shock Proteins / Q000235:genetics*; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D005810:Multigene Family; D011544:Pseudogenes; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D016329:Sp1 Transcription Factor / Q000235:genetics; D016385:TATA Box; D014157:Transcription Factors / Q000235:genetics*
D004268:DNA-Binding Proteins; D000076249:Heat Shock Transcription Factors; D006360:Heat-Shock Proteins; D016329:Sp1 Transcription Factor; D014157:Transcription Factors