Chromosome analysis of human spermatozoa stored in vitro.
Two-hundred-sixty-five chromosome spreads from control human sperm samples capacitated in TEST-yolk buffer at 4 degrees C and 232 chromosome spreads from sperm samples incubated in vitro in Biggers-Whitten-Whittingham (BWW) for 24 h at room temperature prior to capacitation, were studied after fusion of sperm cells with zona-free hamster eggs. Sperm cells were provided by two volunteer donors. The results indicate an increase in chromosome structural abnormalities after in-vitro incubation of the spermatozoa from 1.8 to 7.7% in donor no. 1 and from 4.5 to 12.5% in donor no. 7. Overall, structural abnormalities increased 3.3-fold. The number of aneuploid spermatozoa and the sex ratio did not change significantly. The implications of the use of different media for storing spermatozoa are discussed.
Munné
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Department of Medical Genetics, West Penn Hospital, Pittsburgh, PA 15224.
Estop
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D000328:Adult; D002869:Chromosome Aberrations / Q000209:etiology*; D025063:Chromosome Disorders; D005260:Female; D006801:Humans; D007621:Karyotyping; D008297:Male; D012662:Semen Preservation / Q000379:methods*; D013094:Spermatozoa / Q000502:physiology*