Identification of Leuconostoc spp. by analysis of soluble whole-cell protein patterns.
Leuconostoc spp. share several physiologic characteristics, which sometimes makes it difficult to identify these organisms to the species level. We developed a system, based on the patterns of soluble whole-cell proteins separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, that was able to discriminate between the six Leuconostoc spp. that are capable of growth at 37 degrees C. Nine unidentified Leuconostoc-like bacterial isolates that were included in the study did not fit any of the protein profiles of the type strains and may represent new Leuconostoc spp.
Elliott
J A
JA
Respiratory Diseases Branch, Centers for Disease Control, Atlanta, Georgia 30333.
Facklam
R R
RR
D001426:Bacterial Proteins / Q000032:analysis*; D015373:Bacterial Typing Techniques; D004591:Electrophoresis, Polyacrylamide Gel; D005069:Evaluation Studies as Topic; D006801:Humans; D007934:Leuconostoc / Q000737:chemistry / Q000145:classification* / Q000302:isolation & purification; D012995:Solubility; D013045:Species Specificity
D001426:Bacterial Proteins