Microbial synthesis of 3-deoxy-D-erythro-hex-2-ulosonic acid 6-phosphate.
A microbial route was explored for the synthesis of 3-deoxy-D-erythro-hex-2-ulosonic acid 6-phosphate (2-keto-3-deoxy-6-phosphogluconate, KDPG). Two strains of bacteria, Alcaligenes eutrophus H16 F34 (DSM 529) and Escherichia coli DF 71 (CGSC 4880), lacking in KDPG-aldolase activity were tested for excretion of KDPG. Using pyruvate and gluconate as carbon sources, Alcaligenes eutrophus H16 F34 accumulated and excreted 3-deoxy-D-erythro-hexulosonic acid 6-phosphate into the culture broth, while the E. coli strain, using pyruvate and glucuronate, failed. KDPG was isolated from the culture supernatant of Alcaligenes eutrophus H16 F34 in 78% yield and 5 g scale with respect to the consumed gluconate.
Knappmann
B R
BR
Institut für Enzymtechnologie der Heinrich-Heine Universität Düsseldorf, Jülich, FRG.
el-Nawawy
M A
MA
Schlegel
H G
HG
Kula
M R
MR
D000421:Alcaligenes / Q000378:metabolism*; D000446:Aldehyde-Lyases / Q000235:genetics; D004926:Escherichia coli / Q000378:metabolism*; D005942:Gluconates / Q000138:chemical synthesis* / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D011773:Pyruvates / Q000378:metabolism; D019289:Pyruvic Acid
D005942:Gluconates; D011773:Pyruvates; C081217:2-keto-3-deoxy-6-phosphogluconate; D019289:Pyruvic Acid; D000446:Aldehyde-Lyases; C100131:phospho-2-keto-3-deoxy-gluconate aldolase; C030691:gluconic acid