Pro-major basic protein has three types of sugar chains at the pro-portion.
The amino-acid sequence of purified recombinant pro-major basic protein from Chinese hamster kidney cells was determined to verify the primary structure and glycosylation sites. Reduced and S-carboxamidemethylated protein was first digested with Achromobacter proteinase I. Each peptide was characterized by amino-acid analysis and amino-acid sequence analysis. We could identify all the peptides which were expected from the pro-major basic protein cDNA sequence. Sequence analysis and deglycosylation study revealed that Ser-8, Thr-9, Ser-46 and Asn-70 were glycosylated. The results indicated that proMBP has three types of sugar chains, O-glycoside, N-glycoside and glycosaminoglycan, in the pro-portion.
Tsukuba Research Laboratories, Eisai Company Ltd., Ibaraki, Japan.
Y
Y
Shikata
Y
Y
Hayashi
K
K
Yoshimatsu
Y
Y
Ohya
T
T
Seto
K
K
Fukushima
Y
Y
Yoshida
D000595:Amino Acid Sequence; D000818:Animals; D001216:Asparagine / Q000737:chemistry; D001798:Blood Proteins / Q000737:chemistry* / Q000378:metabolism; D006224:Cricetinae; D047091:Eosinophil Granule Proteins; D006020:Glycopeptides / Q000737:chemistry; D006023:Glycoproteins / Q000737:chemistry* / Q000378:metabolism; D006031:Glycosylation; D008969:Molecular Sequence Data; D009005:Monosaccharides / Q000032:analysis*; D010449:Peptide Mapping; D011498:Protein Precursors / Q000737:chemistry* / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D021382:Protein Sorting Signals / Q000737:chemistry / Q000378:metabolism; D012260:Ribonucleases; D017421:Sequence Analysis; D012694:Serine / Q000737:chemistry; D012697:Serine Endopeptidases / Q000378:metabolism; D013912:Threonine / Q000737:chemistry
D001798:Blood Proteins; D047091:Eosinophil Granule Proteins; D006020:Glycopeptides; D006023:Glycoproteins; D009005:Monosaccharides; D011498:Protein Precursors; D021382:Protein Sorting Signals; D013912:Threonine; D012694:Serine; D001216:Asparagine; D012260:Ribonucleases; C025097:Escherichia coli periplasmic proteinase; D012697:Serine Endopeptidases