In situ quantitative estimates of the age-related and diabetes-related changes in cerebral endothelial barrier antigen.
To determine whether ageing or diabetes, conditions known to alter the blood-brain barrier (BBB) is associated with changes in endothelial barrier antigen (EBA), a rat BBB-specific protein, in situ quantitation of forty vibrotome sections of cerebral tissue of 5 young (4 months old), 5 aged (26 months old) and 5 streptozotocin-induced diabetic rats after 4 weeks of diabetes were studied using immunohistologic techniques. The anti-EBA-stained microvessels were normalized against the total microvessels identified with either anti-EBA binding or anti-Glut-1 binding. The results indicate that the EBA-stained microvessels of the hippocampus, but not of other cerebral cortical areas or white matter, are reduced in aged rats (88.02 +/- 2.19% vs. 80.65 +/- 2.44%, P < 0.01). There were no significant changes in the diabetic rats. These results support the notion that changes in the BBB correlate with the aging of the central nervous system.
St. Louis V.A. Medical Center, MO.
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Mooradian
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Grabau
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Uko-eninn
D000375:Aging / Q000276:immunology / Q000502:physiology*; D000818:Animals; D000954:Antigens, Surface / Q000032:analysis*; D001812:Blood-Brain Barrier / Q000502:physiology*; D002540:Cerebral Cortex / Q000098:blood supply / Q000502:physiology; D002560:Cerebrovascular Circulation / Q000502:physiology; D003921:Diabetes Mellitus, Experimental / Q000276:immunology / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000276:immunology; D051272:Glucose Transporter Type 1; D006624:Hippocampus / Q000098:blood supply / Q000502:physiology; D007150:Immunohistochemistry; D008297:Male; D008833:Microcirculation / Q000502:physiology; D009004:Monosaccharide Transport Proteins / Q000276:immunology / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000032:analysis*; D051381:Rats; D011916:Rats, Inbred F344
D000954:Antigens, Surface; D051272:Glucose Transporter Type 1; D009004:Monosaccharide Transport Proteins; D009419:Nerve Tissue Proteins