Genomic mismatch scanning: a new approach to genetic linkage mapping.
Genomic mismatch scanning (GMS) is a new method of genetic linkage analysis that does not require conventional polymorphic markers or gel electrophoresis. GMS is ideally suited to affected-relative-pair mapping. DNA fragments from all regions of identity-by-descent between two relatives are isolated based on their ability to form extensive mismatch-free hybrid molecules. The genomic origin of this selected pool of DNA fragments is then mapped in a single hybridization step. Here we demonstrate the practicality of GMS in a model organism, Saccharomyces cerevisiae. GMS is likely to be applicable to other organisms, including humans, and may be of particular value in mapping complex genetic traits.
Nelson
S F
SF
Department of Pediatrics, Stanford University Medical Center, California 94305.
McCusker
J H
JH
Sander
M A
MA
Kee
Y
Y
Modrich
P
P
Brown
P O
PO
D002874:Chromosome Mapping / Q000379:methods*; D015825:Chromosomes, Fungal; D002877:Chromosomes, Human; D004271:DNA, Fungal / Q000235:genetics; D005090:Exodeoxyribonucleases; D005240:Feasibility Studies; D008040:Genetic Linkage; D005826:Genetics, Medical / Q000379:methods; D016678:Genome; D016681:Genome, Fungal; D015894:Genome, Human; D006801:Humans; D008745:Methylation; D008780:Methyltransferases; D009693:Nucleic Acid Hybridization; D011110:Polymorphism, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics; D012680:Sensitivity and Specificity; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific)
D004271:DNA, Fungal; D008780:Methyltransferases; C058693:Dam methyltransferase; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific); D005090:Exodeoxyribonucleases; C020133:exodeoxyribonuclease III