Expression of adenosine A1 receptors in cultured neurons from fetal rat brain.
The expression of adenosine A1 receptors was investigated using [3H]2-chloro-N6-cyclopentyladenosine (CCPA) in 8-day-old cultured neurons from fetal rat forebrain grown in serum-free medium. [3H]CCPA bound specifically and with high affinity (Kd = 2.9 nM) to a homogeneous population of sites. Displacement of CCPA binding by various adenosine derivatives indicated that A1 receptors were selectively labeled. The presence of Gpp(NH)p, a GTP analogue, reduced significantly the binding affinity (Kd = 12.2 nM), suggesting that A1 receptors detected in intact cultured cells are linked to associated G proteins.
Nicolas
F
F
INSERM U.272, Nancy, France.
Daval
J L
JL
D000241:Adenosine / Q000031:analogs & derivatives / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000196:embryology / Q000378:metabolism*; D002478:Cells, Cultured; D005333:Fetus / Q000378:metabolism; D009474:Neurons / Q000378:metabolism*; D051381:Rats; D011983:Receptors, Purinergic / Q000378:metabolism*; D012044:Regression Analysis
D011983:Receptors, Purinergic; C058394:2-chloro-N(6)cyclopentyladenosine; D000241:Adenosine