Presence of a nuclear lamina in pachytene spermatocytes of the rat.
The nuclear lamina is a karyoskeletal structure located at the periphery of cell nuclei. The major constituents are the lamins, which belong to the evolutionarily conserved multigene family of intermediate filament proteins. Lamins show a conspicuous cell type-specific expression pattern. The majority of somatic cells of vertebrates express A-type (lamins A and C) as well as B-type (lamins B1 and B2) lamins. Although a lamina structure has been demonstrated to be a ubiquitous component of somatic nuclei its existence in certain meiotic stages during spermatogenesis has been a matter of debate. In this study, we investigated the expression of lamins in rat spermatogenic cells using immunological and protein-chemical methods. We report on the presence of a nuclear lamina structure in rat pachytene spermatocytes. With the aid of a novel broad-reacting lamin antibody we have demonstrated the expression of a protein that is closely related, if not identical, to lamin B1.
Vester
B
B
Department of Cell and Developmental Biology, University of Würzburg, Germany.
Smith
A
A
Krohne
G
G
Benavente
R
R
D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry; D002467:Cell Nucleus / Q000737:chemistry* / Q000648:ultrastructure; D015151:Immunoblotting; D007150:Immunohistochemistry; D034921:Lamin Type B; D034882:Lamins; D008297:Male; D008540:Meiosis; D009687:Nuclear Proteins / Q000032:analysis* / Q000737:chemistry; D010449:Peptide Mapping; D051381:Rats; D017208:Rats, Wistar; D012708:Sertoli Cells / Q000737:chemistry / Q000648:ultrastructure; D013090:Spermatocytes / Q000737:chemistry* / Q000648:ultrastructure; D013091:Spermatogenesis; D014357:Trypsin
D000911:Antibodies, Monoclonal; D034921:Lamin Type B; D034882:Lamins; D009687:Nuclear Proteins; D014357:Trypsin