Detection of T and B cells in many animal species using cross-reactive anti-peptide antibodies.
A wide range of lineage-specific Ag are detectable in the human lymphoid system using mAb, but only a few such markers are detectable in animal species. In this paper, we have investigated the interspecies reactivity of antibodies raised against intracytoplasmic peptide sequences from two T cell Ag (CD3 and CD5) and two B cell markers (the Ig-associated polypeptides encoded by the mb-1 and B29 genes). Immunocytochemical labeling of tissue sections showed that these antibodies cross-react widely between different species (including ungulates, rodents, and marsupials), staining B or T cell areas selectively in lymphoid tissue. The specificity of these antibodies for the animal homologues of the human T and B cell markers was confirmed for the rat by Western blotting analysis. The broad cross-reactivity of these antibodies appears to be due to the fact that they were raised against intracytoplasmic peptide sequences that are highly conserved between humans and rodents, i.e., 80% for mb-1, 85% for CD5, and 100% for CD3 and B29. This strategy should, in the future, widen the range of lineage-associated markers detectable in experimental animals.
Leukaemia Research Fund Immunodiagnostics Unit, John Radcliffe Hospital, Oxford, United Kingdom.
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M
Jones
J L
JL
Cordell
A D
AD
Beyers
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Tse
D Y
DY
Mason
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000276:immunology*; D000954:Antigens, Surface / Q000032:analysis*; D001402:B-Lymphocytes / Q000276:immunology*; D002417:Cattle; D003429:Cross Reactions; D004285:Dogs; D006801:Humans; D008969:Molecular Sequence Data; D010455:Peptides / Q000276:immunology*; D051381:Rats; D012756:Sheep; D013552:Swine; D013601:T-Lymphocytes / Q000276:immunology*
D000906:Antibodies; D000954:Antigens, Surface; D010455:Peptides