Isolation and characterization of a trisialyllactosylceramide, GT3, containing an O-acetylated sialic acid in cod fish brain.
An O-acetylated ganglioside that generated a trisialyllactosylceramide or GT3 by base treatment was found for the first time in cod fish brain. This ganglioside was isolated by high-performance liquid chromatography, and characterized by fast atom bombardment mass spectrometry, and proton nuclear magnetic resonance spectroscopy in addition to chemical analysis. The structure was identified as a modified GT3 in which the external sialic acid is O-acetylated at the C-9 position. The chemical structure is as follows: II3(9-O-Ac-NeuAc2-8NeuAc2-8NeuAc2-3)Lac Cer.
Waki
H
H
Department of Membrane Biochemistry, Tokyo Metropolitan Institute of Gerontology.
Murata
A
A
Kon
K
K
Maruyama
K
K
Kimura
S
S
Ogura
H
H
Ando
S
S
D000107:Acetylation; D000818:Animals; D001923:Brain Chemistry; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D005399:Fishes; D005732:Gangliosides / Q000737:chemistry / Q000302:isolation & purification*; D007790:Lactosylceramides / Q000737:chemistry / Q000302:isolation & purification*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D015394:Molecular Structure; D019158:N-Acetylneuraminic Acid; D012794:Sialic Acids / Q000032:analysis
D005732:Gangliosides; D007790:Lactosylceramides; D012794:Sialic Acids; C047989:trisialosyllactosylceramide; D019158:N-Acetylneuraminic Acid