Frusemide-induced diabetes mellitus in insulin pretreated rabbits as an experimental model.
Single dose of frusemide 0.1 mg/kg orally was administered in 10 rabbits pretreated with soluble insulin 4 IU intravenously. Induction of diabetes after 24 h was confirmed by an increase in blood sugar and glucosylated haemoglobin and a decrease in serum insulin levels. Ten diabetic rabbits which received only frusemide without insulin pretreatment were used as controls, whereas equal number of insulin-pretreated rabbits receiving hydrochlorothiazide 250 mg/kg orally served as the positive control group. All biochemical parameters remained unaltered in the frusemide-treated test group as well as the positive control group after two weeks. The pancreatotoxic effect of frusemide may induce a stable hyperglycaemic state in rabbits which could lead to a useful diabetic model.
Ray
K
K
Department of Pharmacology, Burdwan Medical College.
Sahana
C C
CC
Chaudhuri
S B
SB
Das
D N
DN
Mondal
S
S
Sarkar
D
D
Roy
G
G
Chowdhury
M K
MK
Maity
C R
CR
D000284:Administration, Oral; D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000139:chemically induced*; D005665:Furosemide / Q000008:administration & dosage / Q000633:toxicity*; D007328:Insulin / Q000494:pharmacology*; D008297:Male; D011292:Premedication; D011817:Rabbits
D007328:Insulin; D005665:Furosemide