CD4dull+ hematopoietic progenitor cells in murine bone marrow.
CD4+ cells comprise approximately 3% to 6% of murine bone marrow (BM) cells. The majority are CD4dull+, but there are two distinct sub populations: CD4 brightly positive Gr-1- cells (CD4hiGr-1-) and CD4+ Gr-1+ cells (CD4loGr-1lo). CD4hiGr-1- cells are considered to be mature T cells by cell surface antigen expression and morphology. CD4loGr-1lo cells, which comprise approximately 0.6% of the BM cells, express small amount of B220 and Thy1 antigens. Interestingly, colony-forming units (CFU)-spleen and CFU-C are not enriched in this population. However, when injected into lethally irradiated mice, CD4loGr-1lo cells were shown to differentiate into T-cell, B-cell, and myelo-monocyte lineages when assayed 26 weeks after transplantation. Furthermore, donor-derived CD4loGr-1lo cells were present in the recipients' BM at least 16 weeks after transplantation. These observations suggest that murine CD4loGr-1lo cells in BM have self-renewal capability and retain the ability to differentiate into at least three lineages in long-term hematopoiesis.
Onishi
M
M
Third Department of Internal Medicine, University of Tokyo, Japan.
Nagayoshi
K
K
Kitamura
K
K
Hirai
H
H
Takaku
F
F
Nakauchi
H
H
D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology; D001854:Bone Marrow Cells; D015704:CD4 Antigens / Q000032:analysis*; D002454:Cell Differentiation; D005434:Flow Cytometry; D005472:Fluorouracil / Q000494:pharmacology; D006098:Granulocytes / Q000166:cytology / Q000276:immunology; D006410:Hematopoiesis; D006412:Hematopoietic Stem Cells / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008819:Mice, Nude; D009000:Monocytes / Q000166:cytology / Q000276:immunology; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology
D015704:CD4 Antigens; D005472:Fluorouracil