Concentrations of intracellular sex steroids in human spermatozoa.
In an attempt to determine whether human spermatozoa contain steroid hormones, the immunoreactive levels of progesterone, testosterone, and estradiol-17 beta were measured in the extracts of sonicated specimens of sperm obtained as ejaculates from husbands of infertile couples (n = 67, age 26-44 years). In the range of sperm concentration between 3.0 and 869.6 million per ejaculate (143.2 +/- 13.6 mean +/- SEM), the concentrations were as follows: progesterone 127.3-6685.4 fmol/ml (1434.5 +/- 178.6), testosterone 98.3-2219.1 fmol/ml (611.6 +/- 49.0), and estradiol-17 beta 33.0-678.1 fmol/ml (206.9 +/- 19.7). Using Pearson's correlation coefficient, the steroid hormone levels correlated significantly with the total sperm number (p < .02). The spermatozoa contained small but measurable amounts of the sex steroids.
Chew
P C
PC
Department of Obstetrics and Gynaecology, National University Hospital, Singapore.
Loganath
A
A
Peh
K L
KL
Chow
W P
WP
Gunasegaram
R
R
Ratnam
S S
SS
D000328:Adult; D004958:Estradiol / Q000032:analysis*; D006801:Humans; D008297:Male; D011374:Progesterone / Q000032:analysis*; D011863:Radioimmunoassay; D013076:Sperm Count; D013094:Spermatozoa / Q000737:chemistry*; D013739:Testosterone / Q000032:analysis*
D013739:Testosterone; D011374:Progesterone; D004958:Estradiol