CGRP-immunoreactivity in Merkel cells and nonmyelinated nerve plexuses of dog skin.
In dog skin Merkel cells, calcitonin gene-related peptide (CGRP) immunoreactivity was consistently encountered at the subcellular dense-core granule region. The immunogold particles used as a marker for CGRP, were located specifically in and around the dense-core granules. They were also found in the surrounding cytoplasm close to the dense-core granules. Nerve terminals supplying the Merkel cell were CGRP-negative. CGRP-immunoreactive nonmyelinated nerve fibres were also found in the same specimen. These nonmyelinated nerve fibres were ensheathed by Schwann cell cytoplasm. The gold particles were only located in the dense-core granules of the axis cylinders. It is concluded that a CGRP-like substance predominantly accumulates in the dense-core granules of dog Merkel cells as well as in cutaneous nonmyelinated fibres.
Cheng Chew
S B
SB
Department of Physiology, Faculty of Medicine, Chinese University of Hong Kong, Shatin, N.T.
Leung
P Y
PY
D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000032:analysis*; D004285:Dogs; D000078404:Epidermal Cells; D004817:Epidermis / Q000737:chemistry* / Q000294:innervation; D007150:Immunohistochemistry; D009412:Nerve Fibers / Q000737:chemistry*
D015740:Calcitonin Gene-Related Peptide