[Diagnosis of mycoplasma infections using directed amplification].
Test systems for rapid detection of mycoplasmas in biological samples have been elaborated on the base of the polymerase chain reaction (PCR). Amplification of the conservative rDNA sequences was used for testing of cell cultures for mycoplasmal contamination. Mycoplasma pneumoniae detection system has been developed based on amplification of the species-specific DNA sequences. Inversions of some repeated sequences in the Mycoplasma pneumoniae genome make it possible to run the PCR with a single primer. The revealed spacer length polymorphism for 16S-23S rDNA operons can be used in the mycoplasmas identification.
E Iu
EIu
Zhulanova
M S
MS
Vonskiĭ
V V
VV
Lisin
T N
TN
Efremova
S N
SN
Borkhsenius
D000818:Animals; D001483:Base Sequence; D001717:Birds; D002478:Cells, Cultured; D004269:DNA, Bacterial / Q000235:genetics; D005784:Gene Amplification; D005798:Genes, Bacterial; D006801:Humans; D008969:Molecular Sequence Data; D009175:Mycoplasma Infections / Q000175:diagnosis* / Q000382:microbiology; D009177:Mycoplasma pneumoniae / Q000235:genetics*; D016133:Polymerase Chain Reaction
D004269:DNA, Bacterial
Diagnostika mikoplazmennykh zarazheniĭ s pomoshch'iu napravlennoĭ amplifikatsii.