Virologic markers of human immunodeficiency virus type 1 in cerebrospinal fluid. The HIV Neurobehavioral Research Center Group.
As part of a longitudinal study, 265 cerebrospinal fluid (CSF) specimens from 204 human immunodeficiency virus type 1 (HIV-1)-seropositive subjects and 43 seronegative controls were evaluated. Of the 204 seropositive persons, 78 (38%) had > or = 1 CSF culture positive for HIV-1; the probability of being culture positive increased as the number of CSF samples obtained increased (P = .0018). Significantly correlated with culture positivity were elevations in CSF protein level (P = .014) and CSF white blood cell count (P = .001). Virus was more readily cultured from clarified CSF (89%, 42/47) than from the cellular fraction (30%, 14/47; P < .00001). Amplification of HIV-1 DNA by polymerase chain reaction (PCR) from 25 seropositive persons was positive in 9 (82%) of 11 culture-positive and in 4 (29%) of 14 culture-negative specimens, while amplification of viral RNA detected all 11 culture-positive and 9 (64%) of the 14 culture-negative CSF specimens. These data support the hypothesis that the development of HIV-1-associated neurocognitive disorders are not dependent solely on the presence of HIV-1 within the central nervous system.
Department of Pediatrics, University of California, San Diego, La Jolla 92093.
S A
SA
Spector
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Hsia
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D
Pratt
J
J
Lathey
J A
JA
McCutchan
J E
JE
Alcaraz
J H
JH
Atkinson
S
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Gulevich
M
M
Wallace
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I
Grant
D000328:Adult; D001483:Base Sequence; D015415:Biomarkers; D002474:Cell-Free System; D002478:Cells, Cultured; D004279:DNA, Viral / Q000134:cerebrospinal fluid; D015658:HIV Infections / Q000134:cerebrospinal fluid* / Q000188:drug therapy; D015497:HIV-1 / Q000302:isolation & purification*; D006801:Humans; D008137:Longitudinal Studies; D008969:Molecular Sequence Data; D009010:Monte Carlo Method; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000134:cerebrospinal fluid; D012044:Regression Analysis; D015215:Zidovudine / Q000627:therapeutic use
D015415:Biomarkers; D004279:DNA, Viral; D012367:RNA, Viral; D015215:Zidovudine