Detection of immunoglobulin A in urine specimens from children with Campylobacter-associated diarrhea by a chemiluminescent indicator-based western immunoblot assay.
A Western blot (immunoblot) assay was used to detect Campylobacter-specific immunoglobulin A in urine. Acute-phase urine samples from six children with Campylobacter diarrhea had titers ranging from 2 to 8. The highest titer was detected 4 days postonset. Campylobacter-specific immunoglobulin A was undetectable in the paired convalescent-phase specimens and urine samples from three control children.
Naval Medical Research Institute, Bethesda, Maryland 20889-5055.
S J
SJ
Wu
G
G
Pazzaglia
R L
RL
Haberberger
J J
JJ
Oprandy
D G
DG
Sieckmann
D M
DM
Watts
C
C
Hayes
D000293:Adolescent; D000328:Adult; D015153:Blotting, Western / Q000379:methods* / Q000706:statistics & numerical data; D002167:Campylobacter / Q000145:classification / Q000302:isolation & purification; D002169:Campylobacter Infections / Q000276:immunology* / Q000382:microbiology; D002648:Child; D002675:Child, Preschool; D003967:Diarrhea / Q000276:immunology* / Q000382:microbiology; D005069:Evaluation Studies as Topic; D005260:Female; D006801:Humans; D007070:Immunoglobulin A / Q000652:urine*; D008163:Luminescent Measurements; D008297:Male; D012680:Sensitivity and Specificity; D012703:Serotyping
D007070:Immunoglobulin A