Commercial kits for 1,25-dihydroxyvitamin D compared with a liquid-chromatographic assay.
Three commercially available kits for 1,25-dihydroxyvitamin D [1,25(OH)2D] determination were compared with a liquid-chromatographic method. Serum samples were analyzed for 1,25(OH)2D from 70 healthy subjects (age 2.2-17.5 years; 35 males, 35 females), some (n = 5) given orally high vitamin D3 doses. In addition, 1,25(OH)2D was measured in 28 patients with untreated diseases associated with low (n = 16) or high (n = 12) serum concentrations of the hormone. The results showed that the commercial kits were sufficiently accurate with respect to the comparison method and suitable for routine clinical work.
Bertelloni
S
S
Department of Pediatrics, University of Pisa, Italy.
Baroncelli
G I
GI
Benedetti
U
U
Franchi
G
G
Saggese
G
G
D000293:Adolescent; D002117:Calcitriol / Q000097:blood*; D002648:Child; D002675:Child, Preschool; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D011933:Reagent Kits, Diagnostic / Q000592:standards* / Q000706:statistics & numerical data
D011933:Reagent Kits, Diagnostic; D002117:Calcitriol