Tetanus toxin inhibits depolarization-induced [3H]serotonin release from rat brain cortex synaptosomes.
The effects of tetanus toxin on depolarization-induced [3H]serotonin release from superfused rat brain cortex synaptosomes was investigated. Two hours' preincubation of the synaptosomes with tetanus toxin resulted in a concentration-dependent decrease of K(+)-stimulated release, with an IC50 of about 30 nM (4.5 micrograms/ml); this inhibitory effect was blocked by a previous incubation of the tetanus toxin with antitoxin serum. Tetanus toxin had no effect on reserpine-induced release, a model of Ca(2+)-independent release. These results indicate that tetanus toxin is able to alter the exocytotic machinery of serotoninergic terminals, in agreement with results obtained with other neurotransmitters. They also indicate that serotoninergic terminals possess the receptor for tetanus toxin. These findings are in line with in vivo observations suggesting a role for serotoninergic system in tetanus intoxication.
Istituto di Ricerche Farmacologiche, Mario Negri, Milan, Italy.
M
M
Gobbi
F
F
Facchiano
E
E
Frittoli
A
A
Luini
T
T
Mennini
D000818:Animals; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism*; D005089:Exocytosis / Q000187:drug effects; D066298:In Vitro Techniques; D008297:Male; D051381:Rats; D012110:Reserpine / Q000494:pharmacology; D012701:Serotonin / Q000378:metabolism*; D013574:Synaptosomes / Q000187:drug effects / Q000378:metabolism*; D013744:Tetanus Toxin / Q000494:pharmacology*
D013744:Tetanus Toxin; D012701:Serotonin; D012110:Reserpine