A simple method to detect HIV-1 protein specificity of IgG intrathecal synthesis in HIV-1 infection.
There is currently no simple method to detect the antigen specificity of anti-HIV-1 IgG intrathecal synthesis (IS). Fifty-seven pairs of serum and corresponding CSF from 29 HIV-1 seropositive patients were adjusted to an identical concentration of total IgG and tested by a commercial HIV-1 Western Blot (WB) assay. IgG IS to a given HIV-1 protein was demonstrated when the corresponding band was present in CSF but absent or significantly less represented in serum. A total anti-HIV-1 IS was defined as the presence of an IS to one or more HIV-1 antigens. Our WB analysis of CSF and serum, compared with conventional mathematical formulas, showed a higher sensitivity in demonstrating anti-HIV-1 IgG IS. Moreover, the method disclosed which HIV-1 proteins represent the target of IgG IS. This procedure is easy to perform and therefore may represent a valuable tool to study central nervous system (CNS) involvement by HIV-1 during different stages of infection.
Carbonara
S
S
Clinic of Infectious Diseases, University of Bari, Italy.
Angarano
G
G
Quarto
M
M
Germinario
C
C
Chironna
M
M
Fiore
J R
JR
Monno
L
L
Barbuti
S
S
Pastore
G
G
D000918:Antibody Specificity; D015153:Blotting, Western / Q000379:methods*; D015483:HIV Antibodies / Q000097:blood / Q000134:cerebrospinal fluid*; D015488:HIV Antigens / Q000276:immunology; D006679:HIV Seropositivity / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000134:cerebrospinal fluid; D012680:Sensitivity and Specificity
D015483:HIV Antibodies; D015488:HIV Antigens; D007074:Immunoglobulin G