A scale associated protein of Apedinella radians (Pedinellophyceae) and its possible role in the adhesion of surface components.
Monoclonal antibodies have been generated against cell surface components of the unicellular phytoflagellate Apedinella radians (Pedinellophyceae). One monoclonal antibody, designated Arg 1E5/1B1, labels a scale associated protein (SAP) of 145 kDa. Immunofluorescence microscopy of whole cells as well as immunoelectron microscopy of whole cell mounts and thin sections using Arg 1E5/1B1 have shown that the SAP is located on the proximal surface of body scales and spine-scales. Its specific location suggests that the SAP may play a role in the adhesion of these surface components to the cell membrane and/or to one another. The potential of monoclonal antibody Arg 1E5/1B1 as a tool to study cell surface morphogenesis and the role of the endomembrane system in A. radians is discussed.
Koutoulis
A
A
School of Botany, University of Melbourne, Parkville, Australia.
Ludwig
M
M
Wetherbee
R
R
D000818:Animals; D000911:Antibodies, Monoclonal; D015815:Cell Adhesion Molecules / Q000276:immunology / Q000302:isolation & purification*; D056890:Eukaryota; D009024:Morphogenesis; D015800:Protozoan Proteins / Q000276:immunology / Q000302:isolation & purification*; D013499:Surface Properties
D000911:Antibodies, Monoclonal; D015815:Cell Adhesion Molecules; D015800:Protozoan Proteins