Participation of tyrosine kinase in capping, internalization, and antigen presentation through membrane immunoglobulin in BAL17 B lymphoma cells.
BAL17 cells pulsed with goat anti-IgM or anti-IgD as antigens stimulated a goat IgG specific T cell clone in terms of inositol phosphate production. The antigen-presenting capacity of BAL17 cells was inhibited by pretreatment with the tyrosine kinase inhibitors herbimycin A or genistein. Furthermore, ligand-induced capping and endocytosis of membrane immunoglobulin, monitored at the single cell level, was also blocked by herbimycin A. These results indicate that tyrosine phosphorylation plays an important role in receptor-mediated antigen presentation by B cells.
Department of Immunology and Serology, Tokyo Medical College, Japan.
K
K
Shimo
Y
Y
Gyotoku
Y
Y
Arimitsu
T
T
Kakiuchi
J
J
Mizuguchi
D000903:Antibiotics, Antineoplastic / Q000494:pharmacology; D000938:Antigen-Presenting Cells / Q000201:enzymology / Q000378:metabolism*; D016227:Benzoquinones; D004705:Endocytosis; D004789:Enzyme Activation; D007152:Immunologic Capping; D047029:Lactams, Macrocyclic; D016393:Lymphoma, B-Cell; D011505:Protein-Tyrosine Kinases / Q000378:metabolism*; D011809:Quinones / Q000494:pharmacology; D011947:Receptors, Antigen, B-Cell / Q000378:metabolism*; D017828:Rifabutin / Q000031:analogs & derivatives; D014407:Tumor Cells, Cultured
D000903:Antibiotics, Antineoplastic; D016227:Benzoquinones; D047029:Lactams, Macrocyclic; D011809:Quinones; D011947:Receptors, Antigen, B-Cell; D017828:Rifabutin; C020754:herbimycin; D011505:Protein-Tyrosine Kinases