Simultaneous determination of chloroquine and quinine in human biological fluids by high-performance liquid chromatography.
A high-performance liquid chromatographic method with fluorescence detection is described for the simultaneous measurement of quinine, chloroquine and mono- and bidesethylchloroquine in human plasma, erythrocytes and urine. After a liquid-solid extraction on a Bond Elut C8 cartridge, the compounds are separated on an Inertsil silica column by gradient elution; the mobile phase is a mixture of acetonitrile and methanol-25% ammonia solution (92.7:7.5, v/v). The eluent was monitored with a fluorescence detector (excitation wavelength 325 nm and emission wavelength 375 nm). The limit of detection was ca. 5 ng/ml for chloroquine and ca. 23 ng/ml for quinine. No chromatographic interferences could be detected from endogenous compounds or other antimalarial drugs. The method is accurate with inter- and intra-assay coefficients of variation lower than 7%. Hydroxychloroquine is used as an internal standard because of its structural similarity to chloroquine. The procedure requires 30 min and can be used for therapeutic drug monitoring.
Chaulet
J F
JF
Laboratoire de Biochimie du Professeur Lemontey, Hôpital d'Instruction des Armées Desgenettes, Lyon, France.
Robet
Y
Y
Prevosto
J M
JM
Soares
O
O
Brazier
J L
JL
D002738:Chloroquine / Q000031:analogs & derivatives / Q000032:analysis* / Q000097:blood / Q000652:urine; D002851:Chromatography, High Pressure Liquid; D004912:Erythrocytes / Q000737:chemistry; D006801:Humans; D007202:Indicators and Reagents; D010949:Plasma / Q000737:chemistry; D011803:Quinine / Q000032:analysis* / Q000097:blood / Q000652:urine; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
D007202:Indicators and Reagents; D002738:Chloroquine; D011803:Quinine; C033490:N,N-dideethylchloroquine; C007278:desethylchloroquine