Developing a radioimmunoassay for anordrin: the synthesis of propionyl and hemisuccinyl esters of anordiol.
We describe the chemical synthesis of the 2 beta-propionate-17 beta- hemisuccinate and 2 beta-hemisuccinate-17 beta-propionate diesters of anordiol (2 alpha,17 alpha-diethynyl-A-nor-5 alpha-androstane-2 beta,17 beta-diol) and the method for coupling them to bovine serum albumin and Affi-gel 102, in order to prepare antibodies for radioimmunoassay of anordrin. In addition, we describe the chemical synthesis of the following derivatives: 2 beta-ol-17 beta-propionate, 2 beta-propionate-17 beta-ol, 2 beta-hemisuccinate-17 beta-ol, and 2 beta-ol-17 beta-hemisuccinate.
Vincze
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Population Council, Center for Biomedical Research, New York, NY 10021.
Monder
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D000906:Antibodies; D003281:Contraceptives, Postcoital / Q000032:analysis* / Q000737:chemistry* / Q000276:immunology; D004951:Esterification; D004952:Esters; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D009635:Norandrostanes / Q000032:analysis* / Q000737:chemistry* / Q000276:immunology; D011863:Radioimmunoassay / Q000379:methods*
D000906:Antibodies; D003281:Contraceptives, Postcoital; D004952:Esters; D009635:Norandrostanes; C023615:anordiol; C010855:anordrin