Determination of argininosuccinate lyase in human serum by ion-pair reversed phase liquid chromatography.
A new assay for argininosuccinate lyase based on the separation of the enzymatic reaction mixture components by an ion-pair reversed phase mechanism is reported. The determination of enzyme activity is performed after direct injection by UV detection of the fumarate formed. The chromatographic analysis time is 8 min and a detection limit of 0.4 U/L is achieved. The HPLC method is highly accurate, sensitive and precise. The simple procedure makes this method suitable for the routine determination of ASAL activity in human serum samples.
Department of Chemistry, University of Rome La Sapienza, Italy.
S
S
Canepari
V
V
Carunchio
A M
AM
Girelli
A
A
Messina
D001120:Arginine / Q000378:metabolism; D001123:Argininosuccinate Lyase / Q000097:blood*; D001125:Argininosuccinic Acid / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000706:statistics & numerical data; D005650:Fumarates / Q000378:metabolism; D006801:Humans; D012680:Sensitivity and Specificity
D005650:Fumarates; D001125:Argininosuccinic Acid; C032005:fumaric acid; D001120:Arginine; D001123:Argininosuccinate Lyase