[Morphological examination: immunohistochemical study--study for eosinophils in an autopsy case of asthma using monoclonal antibodies (EG1, EG2)].
In order to investigate the activation of eosinophils in asthma, we performed an immunohistochemical study of the lung and bone marrow obtained from an autopsy case of asthma, using an eosinophil granule mAb (EG2) as a marker of activated secreting eosinophils. We found a large number of EG2-positive eosinophils in and around the small bronchioles and numerous EG2-positive pro-eosinophils in the bone marrow. In this paper, a method of immunohistochemical study using monoclonal antibodies (EG1, EG2) and the usefulness for the estimation of activated eosinophils in the inflammatory tissues is discussed.
Department of Medicine and Physical Therapy, Faculty of Medicine, University of Tokyo.
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Koshino
D000911:Antibodies, Monoclonal; D001249:Asthma / Q000473:pathology*; D001854:Bone Marrow Cells; D004804:Eosinophils / Q000276:immunology; D006801:Humans; D007150:Immunohistochemistry; D008168:Lung / Q000166:cytology*; D008297:Male; D008875:Middle Aged
D000911:Antibodies, Monoclonal