The effects of orthodontic tooth movement on the glycosaminoglycan components of gingival crevicular fluid.
In this study, gingival crevicular fluid (GCF) was collected from around a canine tooth, in children, before and during orthodontic tooth movement. The aim was to identify and quantify the glycosaminoglycan (GAG) components of GCF and relate them to tooth movement, gingival inflammation, plaque accumulation, pocket probing depth and GCF volume recorded at the site of sampling. GAG in GCF samples, collected for a 15-min period into microcapillary tubes, were separated electrophoretically, stained with Alcian blue and quantified using a laser densitometer. 2 GAG components of hyaluronic acid (HA) and chondroitin sulphate (CS) were identified. The increase in GCF volume during orthodontic tooth movement was only partly due to increased gingival inflammation. GAG levels varied with different types of orthodontic tooth movement. In GCF, levels of CS, in particular, may reflect the changes in the deeper periodontal tissues which could be monitored during orthodontic tooth movements.
Department of Clinical Dental Sciences, School of Dentistry, University of Liverpool, UK.
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D000293:Adolescent; D000328:Adult; D016009:Chi-Square Distribution; D002648:Child; D002809:Chondroitin Sulfates / Q000032:analysis; D003481:Cuspid; D003774:Dental Plaque Index; D003799:Dental Stress Analysis; D005260:Female; D005883:Gingival Crevicular Fluid / Q000737:chemistry*; D005891:Gingivitis / Q000209:etiology; D006025:Glycosaminoglycans / Q000032:analysis*; D006801:Humans; D006820:Hyaluronic Acid / Q000032:analysis; D008137:Longitudinal Studies; D008297:Male; D009967:Orthodontic Appliances / Q000009:adverse effects; D009971:Orthodontics, Corrective / Q000009:adverse effects*; D010512:Periodontal Index; D010519:Periodontium / Q000378:metabolism*
D006025:Glycosaminoglycans; D006820:Hyaluronic Acid; D002809:Chondroitin Sulfates