Immunoblot analysis of the placental form of glutathione S-transferase in protein extracted from paraffin-embedded human glioma tissue.
Protein extracted from conventional formalin-fixed and paraffin-embedded tissue sections of human gliomas was examined for immunoblot analysis using antibody against the placental form of glutathione S-transferase (GST-pi). Four benign astrocytomas, five anaplastic astrocytomas and four glioblastomas were used in this study. The preliminary study demonstrated that immunoreactivity of GST-pi was well preserved in normal brain tissue and normal term placenta fixed in acetone, formalin or buffered formalin (pH 7.4). GST-pi in gliomas fixed in formalin also had a good immunoreactivity and showed clear bands on nitrocellulose membranes processed by the method of Western blotting using anti-GST-pi antibody. The results of immunoblot analysis for GST-pi indicate that the intensity of immunoreactivity of benign astrocytoma, anaplastic astrocytoma and glioblastoma increases with the advance of malignancy of these neoplasms. Western blot analysis for GST-pi can be performed using protein extracted from formalin-fixed and paraffin-embedded tissue sections, and the immunoreactive bands can be analyzed quantitatively by densitometric scanning.
Hara
A
A
Department of Neurosurgery, Gifu University School of Medicine, Japan.
Sakai
N
N
Yamada
H
H
Yoshimi
N
N
Tanaka
T
T
Mori
H
H
D015153:Blotting, Western; D001921:Brain / Q000201:enzymology; D001932:Brain Neoplasms / Q000201:enzymology; D005260:Female; D005910:Glioma / Q000201:enzymology*; D005982:Glutathione Transferase / Q000032:analysis*; D006801:Humans; D009363:Neoplasm Proteins / Q000737:chemistry*; D016612:Paraffin Embedding; D010920:Placenta / Q000201:enzymology; D011247:Pregnancy
D009363:Neoplasm Proteins; D005982:Glutathione Transferase