Cloning and functional expression of a brain G-protein-coupled ATP receptor.
A cDNA encoding a novel member of the G-protein-coupled receptor (GCR) superfamily, an ATP receptor, has been isolated from an embryonic chick whole brain cDNA library by hybridization screening. The encoded protein has a sequence of 362 amino acids (41 kDa) and shares no more than 27% amino acid identity with any known GCR. When expressed as a complementary RNA (cRNA) in Xenopus oocytes a slowly-developing inward current was observed in response to application of ATP. The pharmacology of this expressed protein defines it as a P2Y purinoceptor.
Molecular Neurobiology Unit, Royal Free Hospital School of Medicine, London, UK.
T E
TE
Webb
J
J
Simon
B J
BJ
Krishek
A N
AN
Bateson
T G
TG
Smart
B F
BF
King
G
G
Burnstock
E A
EA
Barnard
D000255:Adenosine Triphosphate / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001923:Brain Chemistry; D002642:Chick Embryo; D003001:Cloning, Molecular; D004553:Electric Conductivity; D019204:GTP-Binding Proteins / Q000378:metabolism*; D008969:Molecular Sequence Data; D009865:Oocytes; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011983:Receptors, Purinergic / Q000096:biosynthesis / Q000145:classification / Q000235:genetics*; D011994:Recombinant Proteins / Q000096:biosynthesis; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution; D014158:Transcription, Genetic; D014981:Xenopus
D012333:RNA, Messenger; D011983:Receptors, Purinergic; D011994:Recombinant Proteins; D000255:Adenosine Triphosphate; D019204:GTP-Binding Proteins