[Amidation of proteins and peptides with carboxypeptidase Y].
The native and modified carboxypeptidase Y-catalyzed reaction of acyl transfer of acylamino acid and peptide residues from the corresponding esters to ammonia was studied. Use of the modified carboxypeptidase Y increased the yield of the resulting product. Calcitonin-Leu was transformed into human calcitonin.
Amosova
T N
TN
Popov
A A
AA
Kudriavtseva
N E
NE
Kapitannikov
Iu V
IuV
Rumi
L D
LD
Antonov
V K
VK
D000577:Amides / Q000737:chemistry*; D000595:Amino Acid Sequence; D002116:Calcitonin / Q000737:chemistry; D002268:Carboxypeptidases / Q000737:chemistry*; D006801:Humans; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*
D000577:Amides; D010455:Peptides; D011506:Proteins; D002116:Calcitonin; D002268:Carboxypeptidases; C022633:serine carboxypeptidase
Amidirovanie belkov i peptidov s pomoshch'iu karboksipeptidazy Y.