Purification and characterization of (S)-1,3-butanediol dehydrogenase from Candida parapsilosis.
(S)-1,3-Butanediol (BDO) oxidizing enzyme was purified from Candida parapsilosis IFO 1396, which could produce (R)-1,3-BDO from the racemate. The purified enzyme was an NAD(+)-dependent secondary alcohol dehydrogenase that oxidized (S)-1,3-BDO to 4-hydroxy-2-butanone stereo-specifically.
Tsukuba Research Center, Daicel Chemical Industries, Ltd., Ibaraki, Japan.
H
H
Yamamoto
A
A
Matsuyama
Y
Y
Kobayashi
N
N
Kawada
D000429:Alcohol Oxidoreductases / Q000737:chemistry* / Q000187:drug effects / Q000302:isolation & purification*; D002072:Butylene Glycols / Q000378:metabolism; D002175:Candida / Q000201:enzymology*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D008970:Molecular Weight; D009243:NAD / Q000378:metabolism; D010084:Oxidation-Reduction; D010618:Phenanthrolines / Q000494:pharmacology; D013053:Spectrophotometry; D013379:Substrate Specificity
D002072:Butylene Glycols; D010618:Phenanthrolines; D009243:NAD; C028491:1,3-butylene glycol; D000429:Alcohol Oxidoreductases; C019801:butanediol dehydrogenase; C025205:1,10-phenanthroline