Ion-pair extraction of histamine from biological fluids and tissues for its determination by high-performance liquid chromatography with fluorescence detection.
A procedure for the selective ion-pair extraction of histamine from various biological fluids and tissues by using the ion-pairing agent bis(2-ethylhexyl)hydrogen-phosphoric acid has been developed. This procedure enabled routine determination of histamine in plasma, urine and tissue samples of humans and rats by reversed-phase high-performance liquid chromatography with fluorescence detection using o-phthaldialdehyde.
Department of Gastroenterology and Hepatology, Hannover Medical School, Germany.
D
D
Tsikas
R
R
Velásquez
C
C
Toledano
G
G
Brunner
D000818:Animals; D001826:Body Fluids / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006632:Histamine / Q000032:analysis* / Q000097:blood / Q000652:urine; D006801:Humans; D007477:Ions; D051381:Rats; D013050:Spectrometry, Fluorescence; D013270:Stomach / Q000737:chemistry
D007477:Ions; D006632:Histamine