mdr 1 gene-expression and villin synthesis in a colon cancer cell line differentiated by sodium butyrate.
Sodium butyrate (NaBu) but not dimethylsulfoxide (DMSO) induced the synthesis of villin, a protein of the brush border microvilli cytoskeleton, in a rat colon cancer cell line. Neither NaBu nor DMSO altered mdr 1-mRNA expression or multidrug resistance (MDR)--associated cellular transport of doxorubicin. These results show that mdr 1 gene expression and activity are independent of other brush border proteins induced by differentiating agents at the apical pole of the epithelial cell.
Petit
J M
JM
Research Group on Digestive Cancers, INSERM 252, Faculty of Medicine, Dijon, France.
Chauffert
B
B
Dimanche-Boitrel
M T
MT
Genne
P
P
Duchamp
O
O
Martin
F
F
D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D002087:Butyrates / Q000494:pharmacology*; D020148:Butyric Acid; D002352:Carrier Proteins / Q000096:biosynthesis*; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D002462:Cell Membrane / Q000378:metabolism; D003110:Colonic Neoplasms / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D004317:Doxorubicin / Q000493:pharmacokinetics; D004351:Drug Resistance / Q000235:genetics*; D015870:Gene Expression / Q000235:genetics*; D008562:Membrane Glycoproteins / Q000378:metabolism / Q000502:physiology; D008840:Microfilament Proteins / Q000096:biosynthesis*; D051381:Rats; D011919:Rats, Inbred Strains; D014407:Tumor Cells, Cultured / Q000187:drug effects
D002087:Butyrates; D002352:Carrier Proteins; D008562:Membrane Glycoproteins; D008840:Microfilament Proteins; C026615:villin; D020148:Butyric Acid; D004317:Doxorubicin; D004121:Dimethyl Sulfoxide