Immunodetection of the plum pox virus helper component in infected plants and expression of its gene in transgenic plants.
Tobacco plants (Nicotiana tabacum cv. Xanthi) have been transformed via Agrobacterium tumefaciens vectors, with cDNAs corresponding to the plum pox virus (PPV) cistron 2 encoding helper component (HC-Pro) and with the first two and half cistrons of the PPV genome. Presence of the HC-Pro in PPV-infected plants and transgenic plants transformed with the gene coding for this protein was investigated using specific polyclonal antibodies produced against the PPV HC-Pro. The results suggest that two proteases are involved in the processing of the PPV N-terminal polyprotein to yield a protein of 48 k (HC-Pro). HC-Pro autolytically cleaves at its carboxyl-terminus and a proteolytic activity, probably associated with the protein (P1) encoded by the cistron 1, is required for the cleavage in planta between the proteins derived from cistrons 1 and 2.
Ravelonandro
M
M
INRA, Centre de Recherches de Bordeaux, Villenave d'Ornon, France.
Peyruchaud
O
O
Garrigue
L
L
de Marcillac
G
G
Dunez
J
J
D016960:Agrobacterium tumefaciens; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D010450:Endopeptidases / Q000096:biosynthesis* / Q000235:genetics; D005822:Genetic Vectors; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000382:microbiology*; D010942:Plant Viruses / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification*; D030821:Plants, Genetically Modified; D010947:Plants, Toxic; D010957:Plasmids; D011233:Precipitin Tests; D014176:Protein Biosynthesis; D011499:Protein Processing, Post-Translational; D011817:Rabbits; D014026:Nicotiana; D014158:Transcription, Genetic
D010450:Endopeptidases