Acorn barnacle Megabalanus rosa lectin (BRA-3): cDNA cloning, gene structure and seasonal changes of mRNA and protein levels.
We have isolated cDNA clones coding for a lectin (BRA-3) from the acorn barnacle, Megabalanus rosa. Sequence comparison of the cDNA clones has revealed polymorphism in the BRA-3 mRNA, which results from single-nucleotide (nt) differences at three positions. All three differences are within the coding region and cause conservative amino acid (aa) changes. The BRA-3 gene is composed of four exons, and the three single-nt differences are located on different exons. In addition, the BRA-3 mRNA and BRA-3 protein levels increased during early summer in a similar fashion, indicating that BRA-3 production is regulated mainly at the level of transcription.
Laboratory of Molecular Biology, School of Hygienic Sciences, Kitasato University, Kanagawa, Japan.
N
N
Takamatsu
T
T
Takeda
M
M
Kojima
M
M
Heishi
K
K
Muramoto
H
H
Kamiya
T
T
Shiba
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D005786:Gene Expression Regulation; D006023:Glycoproteins / Q000235:genetics; D037102:Lectins / Q000235:genetics*; D008969:Molecular Sequence Data; D037121:Plant Lectins; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012333:RNA, Messenger / Q000032:analysis; D011975:Receptors, Mitogen / Q000235:genetics; D015183:Restriction Mapping; D012621:Seasons; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D001468:Thoracica / Q000235:genetics*; D014158:Transcription, Genetic
D006023:Glycoproteins; D037102:Lectins; C064593:Megabalanus rosa lectin; D037121:Plant Lectins; D012333:RNA, Messenger; D011975:Receptors, Mitogen