Application of DNA filter hybridization and PCR to distinguish between human and non-human tissues of poor quality.
The present report describes a novel approach for the identification of human or non-human specimens after long-term storage in a badly preserved state. The application of the PCR-technique (polymerase chain reaction) using human-specific primers as well as Southern blot (filter) hybridization of the sample DNA to a primate-specific DNA probe enabled us to extend the positive identification beyond the limits of conventional methods such as serological or morphological examinations.
Institut für Humangenetik, Universität des Saarlandes, Homburg/Saar, Germany.
R
R
Müllenbach
D
D
Makuch
H J
HJ
Wagner
N
N
Blin
D000818:Animals; D015139:Blotting, Southern; D015342:DNA Probes; D004285:Dogs; D006708:Homicide / Q000331:legislation & jurisprudence*; D006801:Humans; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction / Q000379:methods*; D011180:Postmortem Changes; D013045:Species Specificity; D013552:Swine
D015342:DNA Probes