Protective effect of deprenyl against 1-methyl-4-phenylpyridinium neurotoxicity in rat striatum.
Rats were treated with deprenyl for 3 weeks. Afterwards, slices of the corpus striatum were incubated with 1-methyl-4-phenylpyridinium ion (MPP+). Concentrations of dopamine and its metabolite concentrations were assayed. The effect of MPP+ in animals treated with deprenyl was smaller than in controls, indicating that deprenyl protects against MPP+. We also measured superoxide dismutase (SOD) and catalase activities and carbonyl group content of the proteins. Both activities increased in deprenyl-treated rats, and the amount of carbonyl groups was unchanged. These results suggest that the protective effect of deprenyl is independent of the monoamine oxidase-B inhibitor and of the induction of SOD and catalase activities.
Departamento de Bioquimica, Bromatologia y Toxicologia, Facultad de Farmacia, Universidad de Sevilla, Spain.
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ML
Vizuete
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V
Steffen
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Ayala
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Cano
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Machado
D015655:1-Methyl-4-phenylpyridinium / Q000037:antagonists & inhibitors* / Q000633:toxicity; D000818:Animals; D002374:Catalase / Q000378:metabolism; D003342:Corpus Striatum / Q000187:drug effects* / Q000378:metabolism; D004298:Dopamine / Q000378:metabolism; D008297:Male; D008995:Monoamine Oxidase / Q000378:metabolism; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000037:antagonists & inhibitors; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D010084:Oxidation-Reduction; D051381:Rats; D017208:Rats, Wistar; D012642:Selegiline / Q000494:pharmacology / Q000627:therapeutic use*; D013482:Superoxide Dismutase / Q000378:metabolism
D009419:Nerve Tissue Proteins; D012642:Selegiline; D002374:Catalase; D013482:Superoxide Dismutase; D008995:Monoamine Oxidase; D016660:NAD(P)H Dehydrogenase (Quinone); D015655:1-Methyl-4-phenylpyridinium; D004298:Dopamine