Lipoprotein lipase activity in muscle tissue influenced by fatness, fat distribution and insulin in obese females.
Obesity is associated with dyslipidaemia and increased morbidity and mortality from premature atherosclerosis and diabetes mellitus. Particularly, hypertriglyceridaemia is a characteristic finding in patients with obesity. In addition, the elevated levels of triglycerides may be an important risk factor for development of the obesity-related complications. Lipoprotein lipase activity in skeletal muscle tissue (mLPL) has previously been found to be an important factor regulating the concentration of serum triglycerides. To describe the relationship between mLPL, triglycerides and fatness/fat distribution in more detail we have investigated these parameters under basal conditions and during insulin stimulation in 20 obese females. During hyperinsulinaemia (204 microU ml-1) for 4 h the mLPL activity decreased from 528 +/- 52 nmol FFA g-1 to 412 +/- 44 (P < 0.001). Basal mLPL was negatively correlated with serum triglycerides (r = -0.48, P < 0.05) and positively correlated with HDL-cholesterol (r = 0.58, P < 0.01). Employing multiple variance analysis it was found that both BMI and WHR were negatively correlated to mLPL, however, the impaired lipid profile (high triglyceride, low HDL-cholesterol, high FFA) could only be related to BMI and not to WHR in these obese females. However, reduced insulin-action (insulin resistance) was closely related to abdominal fatness determined by WHR both in relation to the insulin-effect on mLPL as well as for the insulin-effect on whole-body glucose metabolism (clamp-study).(ABSTRACT TRUNCATED AT 250 WORDS)
University Clinic for Internal Medicine and Endocrinology, Aarhus Amtssygehus, Denmark.
B
B
Richelsen
S B
SB
Pedersen
T
T
Møller-Pedersen
O
O
Schmitz
N
N
Møller
J D
JD
Børglum
D000273:Adipose Tissue / Q000473:pathology; D000293:Adolescent; D000328:Adult; D001786:Blood Glucose / Q000378:metabolism; D005260:Female; D006801:Humans; D007328:Insulin / Q000494:pharmacology; D007333:Insulin Resistance; D015227:Lipid Peroxidation; D008055:Lipids / Q000097:blood; D008071:Lipoprotein Lipase / Q000378:metabolism*; D008875:Middle Aged; D009132:Muscles / Q000187:drug effects / Q000201:enzymology; D009765:Obesity / Q000097:blood / Q000201:enzymology* / Q000473:pathology
D001786:Blood Glucose; D007328:Insulin; D008055:Lipids; D008071:Lipoprotein Lipase