Transactivation of mouse insulin-like growth factor II (IGF-II) gene promoters by the AP-1 complex.
The mouse insulin-like growth factor II gene (Igf2) is transcribed from three promoters (P1, P2 and P3), and is expressed in a tissue-specific and developmentally regulated fashion; however, little information is available on the transcription factors controlling Igf2 expression. The AP-1 complex is a transcription factor involved in the regulation of a variety of genes, including those encoding certain growth factors. We show that Igf2 P3 is transactivated by AP-1 in a transient expression assay, and that this effect is mediated through two non-consensus AP-1 binding sites characterised by DNA-protein interaction studies. Mutational analysis indicates these sites are required for AP-1 responsiveness and full promoter activity.
Caricasole
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Cancer Research Campaign Growth Factors (Zoology) Oxford, Department of Zoology, University of Oxford, UK.
Ward
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D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D015152:Blotting, Northern; D002454:Cell Differentiation / Q000235:genetics; D002460:Cell Line; D004247:DNA; D007335:Insulin-Like Growth Factor II / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D016755:Proto-Oncogene Proteins c-jun / Q000378:metabolism*; D051381:Rats; D013234:Stem Cells / Q000166:cytology; D015533:Transcriptional Activation
D016755:Proto-Oncogene Proteins c-jun; D007335:Insulin-Like Growth Factor II; D004247:DNA