Decreased atrial natriuretic peptide receptors in the adrenal gland of genetically hypertensive rats.
In view of differential effects of atrial natriuretic peptide (ANP) in different models of hypertension, the present study was undertaken to assess ANP receptors in various target organs of spontaneously hypertensive rats (SHRs) and age-matched normotensive Wistar-Kyoto (WKY) rats. Membranes from kidney, thoracic aorta, and adrenal gland were labeled with [125I]-iodotyrosyl rat atrial peptide (28 amino acid sequence) at 25 degrees C. Each tissue exhibited uniphasic rectangular hyperbolic saturation isotherm indicative of high affinity single species binding sites. While there were no differences in the ANP receptor densities in thoracic aorta and kidney membranes from the two groups, adrenal gland exhibited lower ANP receptors in the SHRs compared to WKY rats. This study suggests that the lower density of ANP receptors in adrenal gland may be one of the underlying contributing factors in genetic hypertension.
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Division of Pharmaceutical Sciences, College of Pharmacy and Health Sciences, Texas Southern University, Houston 77004.
D000311:Adrenal Glands / Q000378:metabolism*; D000818:Animals; D001013:Aorta, Thoracic / Q000378:metabolism; D001665:Binding Sites; D001794:Blood Pressure; D006973:Hypertension / Q000378:metabolism* / Q000503:physiopathology; D007668:Kidney / Q000378:metabolism; D011869:Radioligand Assay; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D017461:Receptors, Atrial Natriuretic Factor / Q000378:metabolism*; D012016:Reference Values
D017461:Receptors, Atrial Natriuretic Factor