Interleukin-1 bioactivity and DNA synthesis in X-irradiated rat testes.
Stage-specific DNA synthesis and interleukin-1 (IL-1) bioactivity were measured in the rat testis at 2, 6, 12 and 25 days after local irradiation with 3 Gy to investigate whether there was any correlation between germ cell DNA synthesis during repopulation and IL-1-like bioactivity in the seminiferous epithelium. DNA synthesis by intermediate and type-B spermatogonia was reduced significantly at 2, 6 and 12 days after irradiation in seminiferous tubules at stages II-III and IV-V. IL-1 bioactivity was increased significantly at 6 and 25 days after irradiation at stages II-VI during repopulation of spermatogonia. At 2, 6 and 25 days after irradiation at stages VIIa-c a significant increase in IL-1 bioactivity was observed that correlated with repair synthesis of DNA. Increased IL-1 bioactivity was also observed at stages IX-XII at 6 days post-irradiation. These observations support the concept that IL-1 is a spermatogonial growth factor which might also stimulate repair synthesis of DNA.
Department of Anatomy, University of Turku, Finland.
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Hakovirta
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Penttilä
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Pöllänen
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Fröysa
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Söder
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Parvinen
D000818:Animals; D046508:Culture Techniques; D004247:DNA / Q000096:biosynthesis*; D004260:DNA Repair; D006133:Growth Substances / Q000502:physiology; D007375:Interleukin-1 / Q000096:biosynthesis* / Q000502:physiology; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013091:Spermatogenesis / Q000502:physiology*; D013737:Testis / Q000166:cytology / Q000378:metabolism* / Q000528:radiation effects; D013936:Thymidine / Q000378:metabolism
D006133:Growth Substances; D007375:Interleukin-1; D004247:DNA; D013936:Thymidine