The Cdk-associated protein Cks1 functions both in G1 and G2 in Saccharomyces cerevisiae.
The CKS1 gene of Saccharomyces cerevisiae encodes a small essential protein shown to interact genetically and physically with the Cdc28 protein kinase. To investigate the specific functions of the CKS1 gene product, conditional temperature-sensitive mutant alleles were generated. The mutations were found to impair the ability of cells to undergo both the G1/S-phase and G2/M-phase transitions of the cell cycle, as well as the ability to bud. Mutants were not defective, however, in their ability to activate Cdc28 kinase as assayed in vitro on the substrate histone H1. It is likely, therefore, that Cks1 mediates a more specialized function of the Cdc28 kinase such as its ability to form specific multimeric complexes or to localize properly in cellular compartments.
Tang
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Department of Molecular Biology, Scripps Research Institute, La Jolla, California 92037.
Reed
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D048868:Adaptor Proteins, Signal Transducing; D000483:Alleles; D000595:Amino Acid Sequence; D001483:Base Sequence; D017489:CDC28 Protein Kinase, S cerevisiae / Q000378:metabolism* / Q000502:physiology; D018797:Cell Cycle Proteins; D002455:Cell Division / Q000502:physiology; D004252:DNA Mutational Analysis; D004926:Escherichia coli / Q000235:genetics; D005434:Flow Cytometry; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics; D016193:G1 Phase / Q000502:physiology*; D016195:G2 Phase / Q000502:physiology*; D015966:Gene Expression Regulation, Fungal / Q000502:physiology*; D005800:Genes, Fungal; D006358:Hot Temperature; D016200:Maturation-Promoting Factor / Q000096:biosynthesis; D008969:Molecular Sequence Data; D009024:Morphogenesis / Q000235:genetics; D016297:Mutagenesis, Site-Directed; D016133:Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013997:Time Factors
D048868:Adaptor Proteins, Signal Transducing; C081185:CKS1 protein, S cerevisiae; D018797:Cell Cycle Proteins; D005656:Fungal Proteins; D029701:Saccharomyces cerevisiae Proteins; D017489:CDC28 Protein Kinase, S cerevisiae; D016200:Maturation-Promoting Factor