Release of bicarbonate from damaged and restituted gastric mucosa in the cat.
BACKGROUND
Gastric mucosal damage leads to luminal alkalinization, but its dependence on mucosal blood flow and acid secretory state of the mucosa is not known. This study examined release of bicarbonate to the gastric lumen and mucosal blood flow in cats after mucosal damage caused by 2 mol/L NaCl and during 90 minutes of epithelial restitution.


METHODS
Bicarbonate was calculated from measurements of pH and PCO2 in the luminal perfusate. Mucosal blood flow was measured with microspheres.


RESULTS
Luminal bicarbonate increased more than twofold after damage in pharmacologically nontreated, pentagastrin-treated, and omeprazole-treated animals (P < 0.001). Luminal bicarbonate thereafter decreased completely to pre-damage level in pentagastrin-treated, partly in nontreated, but remained elevated in omeprazole-treated animals. Mucosal blood flow increased about 100% 15 minutes after damage (P < 0.001), irrespective of secretory state. Bicarbonate availability (arterial [HCO(3-)] x mucosal blood flow) was significantly related to luminal release of bicarbonate from the newly damaged (P < 0.01) but not from the restituted mucosa.


CONCLUSIONS
(1) From the newly damaged mucosa, the luminal release of bicarbonate is related to availability of blood-borne bicarbonate. (2) From acid-stimulated restituted mucosa, the bicarbonate produced by the parietal cells is not released to the lumen, but either consumed within the mucosa by back-diffusing H+ or distributed to the systemic circulation.
Department of Surgery, University of Bergen, Norway.
K
K
Guttu
B
B
Røsok
K
K
Svanes
J E
JE
Grønbech
D000818:Animals; D001639:Bicarbonates / Q000378:metabolism*; D002245:Carbon Dioxide / Q000097:blood; D002415:Cats; D005260:Female; D005745:Gastric Acidity Determination; D005753:Gastric Mucosa / Q000098:blood supply / Q000378:metabolism* / Q000473:pathology*; D006439:Hemodynamics; D008297:Male; D012039:Regional Blood Flow; D012462:Saline Solution, Hypertonic / Q000633:toxicity
D001639:Bicarbonates; D012462:Saline Solution, Hypertonic; D002245:Carbon Dioxide