Ultrastructural localization of prostaglandin endoperoxide synthase (cyclooxygenase) to isolated, purified fractions of guinea pig peritoneal macrophage and line 10 hepatocarcinoma cell lipid bodies.
Subcellular fractions of purified cytoplasmic, nonmembrane-bound lipid bodies were prepared from [3H]-arachidonic acid-labeled guinea pig peritoneal macrophages and line 10 hepatocarcinoma cells. These fractions, which contained [3H]-arachidonyl lipids, were shown to be devoid of contaminating cellular membranes by electron microscopy, and to contain prostaglandin endoperoxide (PGH) synthase by postembedding immunogold electron microscopy. These findings support a proposed role for these lipid-rich organelles in the generation of eicosanoids by oxidative metabolism of arachidonate in the cyclooxygenase pathway of inflammatory and neoplastic cells.
Department of Pathology, Beth Israel Hospital, Boston, MA 02215.
A M
AM
Dvorak
P F
PF
Weller
V S
VS
Harvey
E S
ES
Morgan
H F
HF
Dvorak
D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism; D002469:Cell Separation; D016861:Cyclooxygenase Inhibitors / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism; D006168:Guinea Pigs; D007150:Immunohistochemistry; D008114:Liver Neoplasms, Experimental / Q000201:enzymology* / Q000648:ultrastructure; D008264:Macrophages / Q000201:enzymology* / Q000648:ultrastructure; D016253:Microscopy, Immunoelectron; D015388:Organelles / Q000201:enzymology* / Q000648:ultrastructure; D010529:Peritoneal Cavity / Q000166:cytology; D013347:Subcellular Fractions; D014407:Tumor Cells, Cultured
D016861:Cyclooxygenase Inhibitors; D016718:Arachidonic Acid