Sulphate conjugation of serotonin and N-acetylserotonin in the mosquito, Aedes togoi.
The sulphate conjugation of serotonin and N-acetylserotonin (NAS) was studied in the mosquito, Aedes togoi, using a high-pressure liquid chromatography-radioisotopic procedure. This involved the transfer of the sulphate group from 3'-phosphoadenosine-5'-phosphosulphate (PAP35S) to serotonin or NAS by phenolsulphotransferase (PST). NAS, the acetylated product, is less polar than serotonin but it is a better substrate of PST. Its removal would conceivably be facilitated by sulphate conjugation which renders it more water-soluble. This sequential two-step reaction, comprising N-acetylation of serotonin and sulphate conjugation of the acetylated product (NAS) was also demonstrated in vitro. The developmental profile of PST activity using NAS as substrate showed that peak activity occurred at pupation and increased progressively for a few days after emergence of the adult. Based on the selective inhibition of the "P" form of PST by 2,6-dichloro-4-nitrophenol (DCNP), the sulphate conjugation of NAS appeared to be catalysed by the "M" form of PST.
Department of Biochemistry, Faculty of Medicine, National University of Singapore.
H G
HG
Khoo
K P
KP
Wong
D000330:Aedes / Q000201:enzymology / Q000378:metabolism*; D000818:Animals; D015239:Arylsulfotransferase / Q000187:drug effects / Q000378:metabolism; D006868:Hydrolysis; D009596:Nitrophenols / Q000494:pharmacology; D010724:Phosphoadenosine Phosphosulfate / Q000378:metabolism; D012701:Serotonin / Q000031:analogs & derivatives* / Q000378:metabolism*; D013431:Sulfates / Q000378:metabolism*
D009596:Nitrophenols; D013431:Sulfates; D012701:Serotonin; D010724:Phosphoadenosine Phosphosulfate; C015802:2,6-dichloro-4-nitrophenol; D015239:Arylsulfotransferase; C006389:N-acetylserotonin