Inhibition of an endogenous growth-related proteinase enhances the recovery of a negative growth regulator from cultured human cells.
The possibility that a growth-related proteinase may act by degrading a negative growth regulatory protein has been investigated. Proteinase inhibitors which inhibit the enzyme also enhance the accumulation of the growth regulator by human fibroblasts. The negative growth regulator shows a similar specificity of inhibition of cellular growth to inhibitors of the growth-related proteinase.
Department of Biochemistry, University of Auckland, New Zealand.
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D000818:Animals; D001665:Binding Sites; D002135:Calcium-Binding Proteins; D002352:Carrier Proteins / Q000032:analysis; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002846:Chromatography, Affinity; D004728:Endothelium, Corneal; D005347:Fibroblasts; D005697:Galactosides / Q000378:metabolism*; D006133:Growth Substances / Q000302:isolation & purification*; D006367:HeLa Cells; D006801:Humans; D009004:Monosaccharide Transport Proteins; D033902:Periplasmic Binding Proteins; D011480:Protease Inhibitors / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D002135:Calcium-Binding Proteins; D002352:Carrier Proteins; D005697:Galactosides; D006133:Growth Substances; D009004:Monosaccharide Transport Proteins; D033902:Periplasmic Binding Proteins; D011480:Protease Inhibitors; C007816:beta-galactoside; C023550:galactose-binding protein