Effects of 17 beta-estradiol, progesterone and tamoxifen on in vitro proliferation of human pituitary adenomas: correlation with specific cellular receptors.
Six human pituitary adenoma cultures, characterized for estrogen and progesterone (Pg) receptors, were treated with 17 beta-estradiol (17 beta-E2), Pg and tamoxifen (TAM) at different concentrations, alone and in combination, for 2, 4 and 8 days. Cell proliferation data showed in most cases a stimulating effect of 17 beta-E2 and an inhibitory effect of Pg on cell growth, which appeared to be correlated with specific receptor expression, but independent of pituitary cell hormone content. A marked inhibitory effect of TAM on cell proliferation was present in all cases, but, on the contrary, was independent of estrogen receptor expression.
Caronti
B
B
Department of Neurological Sciences, University La Sapienza, Rome, Italy.
Palladini
G
G
Bevilacqua
M G
MG
Petrangeli
E
E
Fraioli
B
B
Cantore
G
G
Tamburrano
G
G
Carapella
C M
CM
Jaffrain-Rea
M L
ML
D000236:Adenoma / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D000704:Analysis of Variance; D002455:Cell Division / Q000187:drug effects; D004958:Estradiol / Q000494:pharmacology / Q000627:therapeutic use*; D013006:Growth Hormone / Q000096:biosynthesis; D006801:Humans; D010911:Pituitary Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D011374:Progesterone / Q000494:pharmacology / Q000627:therapeutic use*; D011388:Prolactin / Q000096:biosynthesis / Q000097:blood; D015175:Prolactinoma / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D011960:Receptors, Estrogen / Q000096:biosynthesis; D011980:Receptors, Progesterone / Q000096:biosynthesis; D013629:Tamoxifen / Q000494:pharmacology / Q000627:therapeutic use*; D014407:Tumor Cells, Cultured
D011960:Receptors, Estrogen; D011980:Receptors, Progesterone; D013629:Tamoxifen; D011374:Progesterone; D004958:Estradiol; D011388:Prolactin; D013006:Growth Hormone