Oligopeptides of three to five residues derived from uveitopathogenic sites of retinal S-antigen induce experimental autoimmune uveitis (EAU) in Lewis rats.
We have previously reported that microbial peptides having 3 to 6 amino acid sequence homology with uveitopathogenic peptide of retinal S-antigen (S-Ag) are capable of inducing experimental autoimmune uveitis (EAU) in Lewis rats and subhuman primates. T-cells obtained from animals immunized with these microbial peptides proliferate when stimulated with S-Ag peptide in culture and vice versa. We were interested to know whether these 3 to 6 identical amino acid residues by themselves were sufficient to induce EAU in susceptible animals under optimal conditions. For this purpose we have determined the response of synthetic oligopeptides of 3 to 18 amino acid residues for their ability to indice EAU. Here, we report that small synthetic oligopeptides of 3 to 5 amino acid residues of the two uveitopathogenic peptides of S-Ag induced EAU in Lewis rats, although with a higher dose (2000 micrograms/rat) than that of the larger peptides (50 micrograms/rat). These results define the core sequences in the uveitopathogenic peptides of retinal S-Ag.
Molecular Biology Section, National Eye Institute, National Institutes of Health, Bethesda, Maryland 20892.
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Sunil
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Eto
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Singh
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Shinohara
D000595:Amino Acid Sequence; D000818:Animals; D000941:Antigens / Q000276:immunology*; D019393:Arrestin; D001327:Autoimmune Diseases / Q000276:immunology* / Q000473:pathology; D004195:Disease Models, Animal; D005136:Eye Proteins / Q000276:immunology*; D005260:Female; D008198:Lymph Nodes / Q000473:pathology; D008213:Lymphocyte Activation / Q000276:immunology; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000276:immunology; D010446:Peptide Fragments / Q000276:immunology; D051381:Rats; D011917:Rats, Inbred Lew; D014605:Uveitis / Q000276:immunology* / Q000473:pathology
D000941:Antigens; D019393:Arrestin; D005136:Eye Proteins; D009842:Oligopeptides; D010446:Peptide Fragments