Azospirillum brasilense locus coding for phosphoenolpyruvate:fructose phosphotransferase system and global regulation of carbohydrate metabolism.
Mutants of Azospirillum brasilense unable to grow on fructose include ones affected only on fructose (Fru-) and others impaired on many or all carbohydrates through interference with induction of their specific pathways (Car-). Both types of mutants could be complemented by a cosmid in broad-host-range vector pLAFR1 containing a 27.5-kb genomic insert, Car(-)-complementing activity depending on a 2.2-kb fragment, and Fru(-)-complementing activity depending on an overlapping 9.6-kb fragment.
Chattopadhyay
S
S
Department of Biochemistry, Bose Institute, Calcutta, India.
Mukherjee
A
A
Ghosh
S
S
D015806:Azospirillum brasilense / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D050260:Carbohydrate Metabolism; D004790:Enzyme Induction / Q000187:drug effects; D005632:Fructose / Q000494:pharmacology; D015964:Gene Expression Regulation, Bacterial; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000235:genetics*; D015183:Restriction Mapping
D005632:Fructose; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System