New latex reagent using monoclonal antibodies to capsular polysaccharide for reliable identification of both oxacillin-susceptible and oxacillin-resistant Staphylococcus aureus.
A new latex agglutination test (Pastorex Staph-Plus, Sanofi Diagnostics Pasteur), consisting of a mixture of latex particles coated with fibrinogen and immunoglobulin G for the detection of clumping factor and protein A and latex particles sensitized with monoclonal antibodies directed to Staphylococcus aureus serotype 5 and 8 capsular polysaccharides, was compared with three commercially available rapid agglutination methods for the identification of 220 isolates of S. aureus (61 oxacillin resistant) and 128 isolates of coagulase-negative staphylococci. The sensitivity for identification of S. aureus was high with the Pastorex Staph-Plus test (98.6%) compared with those of the other tests, which ranged from 91.8 to 84.5%. Test sensitivities for the identification of oxacillin-resistant S. aureus were as follows: Pastorex Staph-Plus, 95.1%; Pastorex Staph, 73.8%; Staphyslide, 72.1%; and StaphAurex, 49.2%.
Unité Choléra et Vibrions, Institut Pasteur, Paris, France.
J M
JM
Fournier
A
A
Bouvet
D
D
Mathieu
F
F
Nato
A
A
Boutonnier
R
R
Gerbal
P
P
Brunengo
C
C
Saulnier
N
N
Sagot
B
B
Slizewicz
D000911:Antibodies, Monoclonal; D016667:Bacterial Capsules; D015373:Bacterial Typing Techniques / Q000706:statistics & numerical data; D003030:Coagulase / Q000378:metabolism; D005069:Evaluation Studies as Topic; D006801:Humans; D007202:Indicators and Reagents; D007841:Latex Fixation Tests / Q000379:methods* / Q000706:statistics & numerical data; D010068:Oxacillin / Q000494:pharmacology; D010403:Penicillin Resistance; D011135:Polysaccharides, Bacterial / Q000276:immunology; D012680:Sensitivity and Specificity; D013211:Staphylococcus aureus / Q000145:classification* / Q000187:drug effects / Q000276:immunology
D000911:Antibodies, Monoclonal; D003030:Coagulase; D007202:Indicators and Reagents; D011135:Polysaccharides, Bacterial; C054106:capsular polysaccharide, type 5, Staphylococcus aureus; C064836:capsular polysaccharide, type 8, Staphylococcus aureus; D010068:Oxacillin