The multiplicity of enzymatic DNA reduction: a new purification procedure for a DHA reducing protein from potato tubers.
The multiplicity of DHA reducing proteins has been observed by means of a native-PAGE technique in several plant species, thus demonstrating, in accordance with recent literature, that several differently evolved proteins are likely to perform DHA reduction. Moreover, a research strategy coupling the use of native-PAGE with chromatographic separation procedure, tentatively performed in Solanum tuberosum, proved to be a useful tool for the separation and partial identification of the proteins involved in DHA reduction.
Istituto di Botanica, Università di Bari.
M C
MC
De Tullio
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Paciolla
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de Pinto
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L
De Gara
D002852:Chromatography, Ion Exchange; D003683:Dehydroascorbic Acid / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000302:isolation & purification*; D010940:Plant Proteins / Q000302:isolation & purification*; D010945:Plants, Edible / Q000201:enzymology; D011198:Solanum tuberosum / Q000201:enzymology*
D010940:Plant Proteins; D010088:Oxidoreductases; C020666:glutathione dehydrogenase (ascorbate); D003683:Dehydroascorbic Acid