[Phencyclidine receptors in porcine coronary artery].
By using radioligand assay, there was a phencyclidine (Phe) binding site in porcine coronary artery. This binding was specific, reversible, saturable, and stereoselective. The Scatchard analysis showed that the binding site was a single class, with dissociation constant (Kd) and maximum binding (Bmax) of 27.7 +/- 6.9 nmol.L-1 and 0.82 +/- 0.15 pmol/mg protein, respectively. The displacement experiments revealed that the [3H]Phe binding was displaced by nonradioactive Phe, TCP (Phe receptor agonists), and dextrophan (its antagonist). d-INN, a ligand of sigma receptor, had a weaker activity of displacement. These showed a dose-dependent manner. Both etorphine, an agonist of opioid receptor, and N-methyl-D-aspartic acid (NMDA), an excitatory amino acid, failed to displace the binding. These results suggest that the Phe receptors exist in the porcine coronary artery.
Department of Neurobiology, Shanghai Medical University, China.
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D000818:Animals; D001158:Arteries / Q000378:metabolism; D001667:Binding, Competitive; D003331:Coronary Vessels / Q000378:metabolism*; D011869:Radioligand Assay; D017478:Receptors, Phencyclidine / Q000378:metabolism*; D013552:Swine
D017478:Receptors, Phencyclidine