Analysis of phenolic and flavonoid compounds in juice beverages using high-performance liquid chromatography with coulometric array detection.
Analysis of phenolics and flavonoids in juice beverages using reversed-phase HPLC with coulometric array detection is described. Sixteen serial coulometric detectors were used for on-line resolution of co-eluting compounds and generation of voltammetric data. Within each class of compounds, oxidation potential corresponded to specific substitution patterns where: catechol < methoxycatechol < monohydroxyl < methoxyl. Twenty-seven standard compounds were resolved in a 45-min run. The limits of detection were in the low ng/ml range with a linear response range of at least three orders of magnitude. Intra-run retention time variation was < 1% (R.S.D.) and adjacent sensor response ratios varied by < 5% (R.S.D.). The utility of this technique in generating multivariate data for differentiation of juices and juice mixtures is shown.
Gamache
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ESA Inc, Bedford, MA 01730.
Ryan
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Acworth
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IN
D000704:Analysis of Variance; D001628:Beverages / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D002957:Citrus / Q000737:chemistry; D004563:Electrochemistry; D005419:Flavonoids / Q000032:analysis*; D005638:Fruit / Q000737:chemistry*; D010636:Phenols / Q000032:analysis*; D012015:Reference Standards
D005419:Flavonoids; D010636:Phenols