Albumin grafting on to polypropylene by thermal activation.
4-azido-2-nitrophenyl albumin (ANP-albumin) was prepared by reacting 4-fluoro-3-nitrophenyl azide with albumin. The thermal decomposition kinetics of phenyl azide of ANP-albumin was studied at various temperatures by Fourier-Transform Infrared (FTIR) spectroscopy. The decomposition rate of the phenyl azide increased with temperature. The activation energy for the first-order decomposition of the phenyl azide was 128.0 kJ/mol. Albumin was grafted on to polypropylene (PP) films by thermolysis of the azido groups of ANP-albumin with no premodification of the PP surface. The albumin-grafted surface was characterized by electron spectroscopy for chemical analysis (ESCA) and by quantitative determination of platelet adhesion and activation. The bulk concentration of ANP-albumin used for adsorption varied from 0.001 to 30 mg ml-1, and the albumin-adsorbed PP films were incubated at 100 degrees C for up to 7 h. The carbon and nitrogen peaks resulting from the grafted albumin were used to compare the surface albumin concentrations as a function of the concentration of ANP-albumin in the adsorption solution. When the PP film was adsorbed with ANP-albumin at the concentration of 5 mg ml-1 or higher and incubated at 100 degrees C for longer than 5 h, the surface became resistant to platelet adhesion. The ANP-albumin can be grafted on to chemically inert surfaces such as PP surface through simple thermolysis of azido groups to prevent platelet adhesion and activation.
Tseng
Y C
YC
School of Pharmacy, Purdue University, West Lafayette, IN 47907.
Mullins
W M
WM
Park
K
K
D000327:Adsorption; D001386:Azides / Q000138:chemical synthesis / Q000737:chemistry*; D002448:Cell Adhesion; D005583:Fourier Analysis; D006358:Hot Temperature; D006801:Humans; D007700:Kinetics; D008855:Microscopy, Electron, Scanning; D015539:Platelet Activation; D010973:Platelet Adhesiveness; D011126:Polypropylenes / Q000737:chemistry*; D012709:Serum Albumin / Q000138:chemical synthesis / Q000737:chemistry*
C081659:4-azido-2-nitrophenylalbumin; D001386:Azides; D011126:Polypropylenes; D012709:Serum Albumin; C007753:4-fluoro-3-nitrophenyl azide