Cryo-SEM evaluation of desiccation-sensitive dental specimens.
As a result of desiccation, conventionally prepared fresh dental specimens, examined with the scanning electron microscope (SEM), exhibit obvious artifactual effects that may include one or more of the following: distortion, fracture, separation of structures, and chemical interactions. Cryo-SEM techniques reduce or eliminate these artifacts and present reliable images while requiring less time to perform than do conventional methods.
Driessen
C H
CH
Department of Restorative Dentistry, University of Pretoria, South Africa.
Coetzee
J
J
D000293:Adolescent; D000328:Adult; D016477:Artifacts; D015925:Cryopreservation / Q000379:methods*; D003804:Dentin / Q000648:ultrastructure*; D005260:Female; D005899:Glass Ionomer Cements; D006801:Humans; D008297:Male; D008855:Microscopy, Electron, Scanning / Q000379:methods*; D008964:Molar, Third / Q000648:ultrastructure; D016628:Replica Techniques; D015203:Reproducibility of Results; D012107:Research Design
D005899:Glass Ionomer Cements