Induction and suppression of cytokine release (tumour necrosis factor-alpha; interleukin-6, interleukin-1 beta) by Escherichia coli pathogenicity factors (adhesions, alpha-haemolysin).
Escherichia coli bacteria expressing mannose-resistant fimbriae/haemagglutination induced the production of substantial amounts of tumour necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6) from a peripheral human lymphocyte, monocyte, basophil (LMB) cell suspension. In this regard, E. coli bacteria with S-mannose-resistant fimbriae/haemagglutination were the most potent inducers of IL-6 and TNF-alpha secretion, followed by the E. coli strain with P-mannose-resistant fimbriae/haemagglutination. The E. coli alpha-haemolysin did not stimulate cytokine release from human LMB. In fact, this toxin, at non-toxic concentrations, depressed the spontaneous as well as the E. coli-induced production of TNF-alpha, IL-6, IL-1 beta. Our results indicate that two mechanisms may contribute to the severity of E. coli infection: (a) stimulation of cytokine release by type-specific fimbriae/haemagglutination properties and (b) depression of immune response by the E. coli alpha-haemolysin.
Lehrstuhl für Med. Mikrobiologie und Immunologie, Ruhr-Universität Bochum, Germany.
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D001422:Bacterial Adhesion / Q000276:immunology; D001426:Bacterial Proteins / Q000276:immunology; D002478:Cells, Cultured; D016207:Cytokines / Q000235:genetics; D004306:Dose-Response Relationship, Immunologic; D004926:Escherichia coli; D004927:Escherichia coli Infections / Q000276:immunology*; D029968:Escherichia coli Proteins; D006460:Hemolysin Proteins / Q000276:immunology; D006801:Humans; D007375:Interleukin-1 / Q000096:biosynthesis*; D015850:Interleukin-6 / Q000096:biosynthesis*; D007962:Leukocytes / Q000276:immunology; D012333:RNA, Messenger / Q000032:analysis; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*
D001426:Bacterial Proteins; D016207:Cytokines; D029968:Escherichia coli Proteins; D006460:Hemolysin Proteins; C036507:Hlya protein, E coli; D007375:Interleukin-1; D015850:Interleukin-6; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha