Deficiency of the gene B impairs differentiation of melanophores in the medaka fish, Oryzias latipes: fine structure studies.
In an orange-colored variant of the medaka fish, Oryzias latipes, which is homozygous for b allele, the melanophores represent a tissue-specific differentiation, manifesting an amelanotic appearance in the skin, an incomplete melanogenesis in the choroid and the peritoneum, and mosaic phenotype-like melano-iridophores in the peritoneum. In a wild-type strain of this species carrying the B gene, all melanophores are terminally differentiated irrespective of the tissues in which they are located. This indicates that the deficiency of B gene impairs the differentiation of melanophores in the medaka. Electron microscopy disclosed that the deficiency of B gene causes deterioration of melanogenesis to occur inside the melanosomes and that the manner of deterioration in the melanophores in the skin, the choroid and the peritoneum is different. The ubiquitous occurrence of reflecting platelet-laden melanophores in the peritoneum of this variant and the total absence of a mosaicism in pigment cells of the wild-type strain indicate that the deficiency of B gene predestines melanoblasts distributed in this tissue to an ambiguous state with regard to their differentiation. Little difference is observed between melanosomes maturation in pigment epithelial cells of the orange-colored variant and the wild-type strain, indicating an innocent role of the B gene in their differentiation.
Department of Biology, Keio University, Yokohama, Japan.
E
E
Hirose
J
J
Matsumoto
D000818:Animals; D002454:Cell Differentiation / Q000235:genetics; D006801:Humans; D007231:Infant, Newborn; D008544:Melanocytes / Q000648:ultrastructure*; D008547:Melanophores / Q000166:cytology* / Q000648:ultrastructure; D014442:Monophenol Monooxygenase / Q000032:analysis; D009990:Oryzias / Q000235:genetics*
D014442:Monophenol Monooxygenase