Corticosteroidogenesis in the toad Bufo arenarum H: evidence for a precursor role for an aldosterone 3 beta-hydroxy-5-ene analogue (3 beta, 11 beta, 21-trihydroxy-20-oxo-5-pregnen-18-al).
A material isolated following pregnenolone incubations with toad (Bufo arenarum) inter-renal tissue at 28 degrees C has been identified as a 3 beta-hydroxy-5-ene analogue of aldosterone (3 beta, 11 beta, 21-trihydroxy-20-oxo-5-pregnen-18-al). The initial identification was made by enzymic and m.s. methods, and structural confirmation was achieved through comparison with chemically synthesized authentic material. The relative efficacy of corticosterone, 18-hydroxycorticosterone and the 3 beta-hydroxy-5-ene aldosterone analogue as aldosterone precursors was evaluated. In the in vitro situation studied, the 3 beta-hydroxy-5-ene steroid was by far the best precursor.
PRHOM-CONICET, Buenos Aires, Argentina.
N R
NR
Ceballos
C H
CH
Shackleton
M
M
Harnik
E N
EN
Cozza
E G
EG
Gros
C P
CP
Lantos
D015096:3-Hydroxysteroid Dehydrogenases / Q000378:metabolism; D000450:Aldosterone / Q000031:analogs & derivatives* / Q000096:biosynthesis* / Q000378:metabolism; D000818:Animals; D002022:Bufo arenarum; D008401:Gas Chromatography-Mass Spectrometry; D007668:Kidney / Q000378:metabolism
C081376:3 beta,11 beta, 21-trihydroxy-20-oxo-5-pregnen-18-al; D000450:Aldosterone; D015096:3-Hydroxysteroid Dehydrogenases