Occurrence of IgE antibodies against the 39-kDa allergen component of the mite Lepidoglyphus destructor in urban and rural subjects.
Several species of storage mite have been shown to cause IgE-mediated sensitization. Monoclonal antibodies (mabs) have been raised against the storage mite Lepidoglyphus destructor, and one mab (42B6) immunoprecipitated a band of 39 kDa of L. destructor. To search for the frequency of IgE antibodies in patient sera against this allergen component, we modified a radioimmunoassay (RIA), 42B6 being used as capturing antibody. Twenty of 43 sera from farmers who were RAST positive to L. destructor (46.5%) were scored as positive in this assay. There was a moderate degree of correlation between positive RAST results to L. destructor and the 39-kDa allergen. Among 14 sera from Stockholm residents positive in RAST to both Dermatophagoides pteronyssinus and L. destructor, six detected the 39-kDa allergen component. However, three sera from urban subjects lacking IgE antibodies against both mite species also had IgE antibodies against the 39-kDa allergen. The present study demonstrates that the 39-kDa allergen of L. destructor is an important allergen, and that rural as well as urban subjects can become sensitized.
Department of Clinical Immunology, Karolinska Institute, Karolinska Hospital, Stockholm, Sweden.
M
M
van Hage-Hamsten
E
E
Lagging
B
B
Härfast
S G
SG
Johansson
D000293:Adolescent; D000328:Adult; D000485:Allergens / Q000276:immunology*; D000818:Animals; D005260:Female; D006801:Humans; D006967:Hypersensitivity / Q000175:diagnosis / Q000276:immunology; D007073:Immunoglobulin E / Q000032:analysis*; D008297:Male; D008875:Middle Aged; D008925:Mites / Q000276:immunology*; D008970:Molecular Weight; D011852:Radioallergosorbent Test; D012423:Rural Health; D014504:Urban Health
D000485:Allergens; D007073:Immunoglobulin E