Co-agglutination (Co-A) test for circulating antigen in hydatid disease.
A co-agglutination (Co-A) test with Staphylococcus aureus (Cowan's strain I) bearing protein A coated with specific hydatid antibodies, was used to demonstrate circulating hydatid antigen in serum for the diagnosis of hydatid disease. The test had a sensitivity of 95% and specificity of 89% in detecting hydatid antigen in serum. A false positive reaction was observed with 18.5% of control sera from patients with various parasitic diseases. However, these all gave negative results when tested after further dilution. The test is reliable and rapid; the result could be obtained within 30-45 min of receipt of a serum sample. Since the test is simple and the reagents are inexpensive and easily available, it can be used in less well equipped laboratories in developing countries.
Shariff
M
M
Department of Paediatrics, AIIMS, New Delhi, India.
Parija
S C
SC
D000372:Agglutination Tests; D000909:Antibodies, Helminth / Q000097:blood / Q000276:immunology; D000947:Antigens, Helminth / Q000097:blood* / Q000276:immunology; D004443:Echinococcosis / Q000175:diagnosis* / Q000276:immunology; D005069:Evaluation Studies as Topic; D005189:False Positive Reactions; D006801:Humans; D007194:India / Q000453:epidemiology; D012680:Sensitivity and Specificity; D013205:Staphylococcal Protein A / Q000276:immunology; D013211:Staphylococcus aureus / Q000276:immunology
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D013205:Staphylococcal Protein A