Partial structure of the gene encoding the 78 kDa gastrin binding protein excludes a close relationship with the peroxisomal trifunctional enzyme.
A 10.9 kb porcine genomic clone encoding nucleotides 124-732 of the cDNA for the porcine 78 kDa gastrin-binding protein has been isolated and characterized. The coding sequence is interrupted by 7 introns, which vary in length from 93 to 3000bp. The positions of the intron/exon junctions are different from the junctions in the gene encoding the rat peroxisomal trifunctional enzyme. Despite 33% amino acid sequence identity between the two proteins it is concluded that the porcine gastrin binding protein is not closely related to the rat trifunctional enzyme.
Ludwig Institute for Cancer Research, Royal Melbourne Hospital, Victoria, Australia.
G S
GS
Baldwin
A
A
Casey
J
J
Weinstock
D015094:3-Hydroxyacyl CoA Dehydrogenases / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D004247:DNA; D004746:Enoyl-CoA Hydratase / Q000235:genetics*; D005091:Exons; D005755:Gastrins / Q000378:metabolism*; D015698:Genomic Library; D007438:Introns; D007535:Isomerases / Q000235:genetics*; D063991:Mitochondrial Trifunctional Protein; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009097:Multienzyme Complexes / Q000235:genetics*; D063994:Peroxisomal Bifunctional Enzyme; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D013552:Swine
D002352:Carrier Proteins; D005755:Gastrins; D009097:Multienzyme Complexes; D004247:DNA; D015094:3-Hydroxyacyl CoA Dehydrogenases; D063991:Mitochondrial Trifunctional Protein; D004746:Enoyl-CoA Hydratase; D063994:Peroxisomal Bifunctional Enzyme; D007535:Isomerases