Cytotoxic effector cell granules recognized by the monoclonal antibody TIA-1 are present in CD8+ lymphocytes in lymph nodes of human immunodeficiency virus-1-infected patients.
A novel monoclonal antibody (mAB) TIA-1, which recognizes a 15-kd granule-associated protein of cytotoxic T lymphocytes and natural killer cells, has been applied to sections of lymph nodes with human immunodeficiency virus (HIV)-induced lymphadenopathy (follicular hyperplasia and lymphocyte depletion). The protein recognized by this mAB induces apoptosis in permeabilized lymphocytes in vitro. While this mAB reacted with approximately 46% of paracortical CD8+ cells in control nodes, it reacted with 75% of such cells in HIV-induced follicular hyperplasia. Germinal centers of the control nodes contained few TIA-1 + cells; in follicular hyperplasia caused by HIV-1, almost all germinal center CD8+ cells were TIA-1 +. Both in the control nodes and in HIV-induced follicular hyperplasia the majority of TIA-1 + cells coexpressed CD45R0. A marked loss of CD8+TIA-1+ cells was seen in lymphocyte-depleted nodes of patients with AIDS. The loss of these cytotoxic T lymphocytes may have a significant impact on the progression of the disease.
Department of Hematology, Allgemeines Krankenhaus St. Georg. Hamburg, Germany.
K
K
Tenner-Racz
P
P
Racz
C
C
Thomé
C G
CG
Meyer
P J
PJ
Anderson
S F
SF
Schlossman
N L
NL
Letvin
D000163:Acquired Immunodeficiency Syndrome / Q000276:immunology / Q000473:pathology*; D000328:Adult; D000911:Antibodies, Monoclonal / Q000032:analysis* / Q000276:immunology; D016827:CD8 Antigens / Q000032:analysis*; D003594:Cytoplasmic Granules / Q000737:chemistry* / Q000276:immunology / Q000648:ultrastructure*; D005260:Female; D006801:Humans; D006965:Hyperplasia / Q000473:pathology; D007150:Immunohistochemistry; D007694:Killer Cells, Natural / Q000276:immunology / Q000473:pathology / Q000648:ultrastructure; D008198:Lymph Nodes / Q000276:immunology* / Q000473:pathology* / Q000648:ultrastructure; D008212:Lymphocyte Depletion; D008297:Male; D010641:Phenotype; D013601:T-Lymphocytes / Q000276:immunology* / Q000473:pathology* / Q000648:ultrastructure; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology / Q000473:pathology / Q000648:ultrastructure*
D000911:Antibodies, Monoclonal; D016827:CD8 Antigens