PDC activity and acetyl group accumulation in skeletal muscle during isometric contraction.
The activity of pyruvate dehydrogenase complex (PDC) was studied in the human quadriceps femoris muscle during isometric contraction induced by intermittent electrical stimulation at 20 Hz. Muscle biopsy samples were obtained at rest and after 10, 20, and 46 contractions. The active form of PDC (PDCa) increased from a mean value of 26% of the total PDC at rest to mean values of 46, 78, and 80%, respectively. Muscle biopsy samples were also obtained at rest, after 46 contractions with limb blood flow intact or occluded, and after 2 min of oxidative recovery. In another experiment, muscle biopsy samples were obtained at rest, after 10 min of resting ischemia, and after 46 contractions with limb blood flow occluded. The transformation of PDC to PDCa was nearly complete, regardless of whether the blood flow was intact or occluded. However, the accumulation of acetyl groups observed during stimulation with intact blood flow was abolished when the blood flow was occluded. The absence of NADH oxidation during anoxia had no effect on the contraction-induced transformation of PDC to PDCa, but it inhibited the flux through the enzyme reaction.
Department of Clinical Chemistry, Huddinge University Hospital, Karolinska Institute, Sweden.
D
D
Constantin-Teodosiu
G
G
Cederblad
E
E
Hultman
D000105:Acetyl Coenzyme A / Q000378:metabolism; D000107:Acetylation; D000108:Acetylcarnitine / Q000378:metabolism; D000328:Adult; D004789:Enzyme Activation; D005260:Female; D006801:Humans; D007537:Isometric Contraction / Q000502:physiology*; D007700:Kinetics; D008297:Male; D009132:Muscles / Q000378:metabolism*; D009243:NAD / Q000378:metabolism; D011768:Pyruvate Dehydrogenase Complex / Q000378:metabolism*
D011768:Pyruvate Dehydrogenase Complex; D009243:NAD; D000108:Acetylcarnitine; D000105:Acetyl Coenzyme A