Focal increases in [3H]forskolin and [3H]phorbol 12,13-dibutyrate binding in the rat brain following lesions of the medial septum.
Quantitative autoradiography of [3H]forskolin binding to GS-adenylate cyclase and [3H]phorbol 12,13-dibutyrate (PDBu) binding to protein kinase C (PKC) was examined 21 days following ibotenate lesion of the rat medial septum. A significant reduction (-19%) in [3H]forskolin binding was observed at the lesion site in the medial septum compared to the sham-treated group. A significant increase in [3H]forskolin binding was demonstrated in the polymorph layer of the detante gyrus (19%) in animals with medial septal lesions whilst in all other brain regions, [3H]forskolin binding remained unaltered post-lesion. [3H]PDBu binding was significantly increased in the superficial layers (I-III) of entorhinal cortex (27%) following lesion of the medial septum, and remained unaltered in all other brain regions post-lesion. The nature and location of the alterations (namely elevations) in ligand binding sites remote from the lesioned area are supportive of plastic modifications of second messenger systems following denervation.
Wellcome Surgical Institute, University of Glasgow, UK.
K
K
Horsburgh
F M
FM
Inglis
J
J
McCulloch
D000262:Adenylyl Cyclases / Q000378:metabolism; D000818:Animals; D001345:Autoradiography; D001923:Brain Chemistry / Q000187:drug effects*; D001930:Brain Injuries / Q000201:enzymology / Q000378:metabolism*; D005576:Colforsin / Q000378:metabolism*; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism; D006651:Histocytochemistry; D007051:Ibotenic Acid / Q000494:pharmacology; D008024:Ligands; D008297:Male; D015240:Phorbol 12,13-Dibutyrate / Q000378:metabolism*; D011485:Protein Binding; D011493:Protein Kinase C / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D015290:Second Messenger Systems / Q000187:drug effects; D013238:Stereotaxic Techniques
D008024:Ligands; D005576:Colforsin; D007051:Ibotenic Acid; D015240:Phorbol 12,13-Dibutyrate; D011493:Protein Kinase C; D000262:Adenylyl Cyclases