Alanyl glutamine-enriched total parenteral nutrition restores intestinal adaptation after either proximal or distal massive resection in rats.
This study was designed to determine whether alanyl glutamine-containing total parental nutrition (TPN) can restore the impaired adaptive process of the remaining intestine, observed with administration of conventional TPN, after massive small-bowel resection. Seventy-four male Sprague-Dawley rats weighing 250 g were randomly divided into seven groups. Group I rats (n = 10) were killed after overnight fasting. Group II animals (n = 32) underwent massive small bowel resection (85%) with preservation of the first 15 cm of jejunum. Group III animals (n = 32) were also submitted to massive small-bowel resection with preservation of 15 cm of terminal ileum. Three different TPN solutions were prepared. Solution A was a conventional formulation that did not contain glutamine. Solution B contained 1.88 times the amino acid concentration of solution A. Solution C was prepared by adding alanyl glutamine (2 g/100 mL) to solution A. Solutions B and C were isonitrogenous and isocaloric. Each solution was infused to groups II and III, which were subdivided into groups IIA (n = 10), IIB (n = 11), IIC (n = 11), IIIA (n = 10), IIIB (n = 11), and IIIC (n = 11). After 1 week of TPN (270 kcal/kg per day), the experimental animals were killed and the intestine was taken for examination. Final body weight did not differ significantly among the groups, and there was no difference in nitrogen balance among the animals that received solution B or C.(ABSTRACT TRUNCATED AT 250 WORDS)
Department of Pediatric Surgery, Osaka University Medical School, Japan.
H
H
Tamada
R
R
Nezu
Y
Y
Matsuo
I
I
Imamura
Y
Y
Takagi
A
A
Okada
D000222:Adaptation, Physiological / Q000187:drug effects; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D004151:Dipeptides / Q000008:administration & dosage / Q000494:pharmacology*; D007082:Ileum / Q000201:enzymology / Q000502:physiology / Q000601:surgery*; D007413:Intestinal Mucosa / Q000201:enzymology / Q000502:physiology / Q000601:surgery; D007583:Jejunum / Q000502:physiology / Q000601:surgery*; D008297:Male; D009929:Organ Size / Q000187:drug effects; D010289:Parenteral Nutrition, Total; D051381:Rats; D017207:Rats, Sprague-Dawley; D000520:alpha-Glucosidases / Q000378:metabolism
D004151:Dipeptides; D000520:alpha-Glucosidases; C054122:alanylglutamine