Carrier-mediated residual K+ and Na+ transport of human red blood cells.
Residual, i.e., (ouabain, bumetanide, and EGTA)-insensitive K+ and Na+ influxes as well as effluxes of human red blood cells are enhanced in isotonic solutions of low (NaCl + KCl) concentration using sucrose to maintain constant osmolarity. Various carrier models were tested to fit the experimental data of these fluxes simultaneously. The residual K+ and Na+ fluxes can be described on the basis of a carrier mechanism of competing substrates with modifier sites.
Denner
K
K
Department of Biology, Humboldt University Berlin, Germany.
Heinrich
R
R
Bernhardt
I
I
D001692:Biological Transport / Q000502:physiology; D002478:Cells, Cultured; D004910:Erythrocyte Membrane / Q000502:physiology / Q000648:ultrastructure; D004912:Erythrocytes / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D006801:Humans; D007552:Isotonic Solutions; D008433:Mathematics; D008564:Membrane Potentials / Q000502:physiology; D010783:Photometry; D011188:Potassium / Q000493:pharmacokinetics*; D011189:Potassium Chloride / Q000494:pharmacology; D012964:Sodium / Q000493:pharmacokinetics*; D012965:Sodium Chloride / Q000494:pharmacology
D007552:Isotonic Solutions; D012965:Sodium Chloride; D011189:Potassium Chloride; D012964:Sodium; D011188:Potassium