Binding of low mobility group proteins with DNA examined in homologous and heterologous systems by gel retardation assay.
The interaction of low mobility group proteins (LMG), isolated from chromatin of pancreatic carcinoma cells (CAPAN-2), with fragments of 5'-flanking region of the antigen 17-1A gene was studied by gel retardation assay. The LMG proteins, which formed complexes with DNA were extracted from the gels and identified by polyacrylamide gel electrophoresis under denaturing conditions. The proteins of Mw about 100, 60, 55 and 48 kDa, which formed specific complexes with fragments of 5'-flanking region of the antigen 17-1A gene, were identified.
Institute of Human Genetics, Polish Academy of Sciences, Poznan.
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Wiland
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Siemieniako
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Trzeciak
D000818:Animals; D000951:Antigens, Neoplasm / Q000235:genetics; D001483:Base Sequence; D002843:Chromatin / Q000737:chemistry; D004273:DNA, Neoplasm / Q000032:analysis / Q000235:genetics* / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009363:Neoplasm Proteins / Q000032:analysis / Q000235:genetics* / Q000378:metabolism*; D009841:Oligonucleotides; D010190:Pancreatic Neoplasms / Q000737:chemistry / Q000276:immunology / Q000473:pathology; D011485:Protein Binding; D051381:Rats; D014407:Tumor Cells, Cultured
D000951:Antigens, Neoplasm; D002843:Chromatin; D004273:DNA, Neoplasm; D009363:Neoplasm Proteins; D009841:Oligonucleotides