Interleukin-5 mRNA in three T-cell lymphomas with eosinophilia.
The objective of this controlled pilot study was to determine if mRNA coding for interleukin-5 (IL-5), a cytokine that promotes eosinophil differentiation, growth, and migration, could be detected in three T-cell lymphomas that were infiltrated extensively by eosinophils. To detect mRNA coding for IL-5, we performed an RNA polymerase chain reaction on mRNA extracted from three T-cell lymphomas with eosinophilia and from 29 positive and negative validation controls. Using this procedure, we detected a 293-base pair, IL-5-specific amplification product in the three cases of T-cell lymphoma with eosinophilia and in 11 of 12 positive validation controls, including 10 cases of Hodgkin's disease with eosinophilia. IL-5 mRNA was not detectable in the 17 negative validation controls. This preliminary study suggests that IL-5 mRNA is detectable by polymerase chain reaction in three cases of T-cell lymphoma with eosinophilia.
Pathology Department, University of California, Irvine 92717.
M
M
Samoszuk
E
E
Ramzi
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DL
Cooper
D000199:Actins / Q000235:genetics; D002461:Cell Line, Transformed; D004802:Eosinophilia; D006689:Hodgkin Disease / Q000235:genetics / Q000378:metabolism; D006801:Humans; D015848:Interleukin-5 / Q000235:genetics*; D016399:Lymphoma, T-Cell / Q000235:genetics* / Q000378:metabolism; D010865:Pilot Projects; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism*
D000199:Actins; D015848:Interleukin-5; D012333:RNA, Messenger