Parasympathetic cerebrovascular innervation: an anterograde tracing from the sphenopalatine ganglion in the rat.
To elucidate parasympathetic cerebrovascular innervation of the rat sphenopalatine ganglion (SPG), we injected wheat germ agglutinin-conjugated horseradish peroxidase into the SPG of one side and traced anterogradely labeled nerve fibers. Three days after the injection, the animals were killed and tissues including the SPG, major cerebral arteries, and ethmoidal arteries were reacted by the tetramethylbenzidine method. A number of cells and nerve fibers labeled with wheat germ agglutinin-conjugated horseradish peroxidase were observed in the SPG. The nasal mucous membrane, the periorbital soft tissue, and the lacrimal gland of the injected side contained numerous labeled nerve fibers. In cerebral vessels, anterogradely labeled nerve fibers were observed around the internal ethmoidal, anterior cerebral, middle cerebral, internal carotid, and posterior cerebral arteries of both sides. A few labeled nerve fibers were seen on the wall of the basilar arteries of the distal portion, and the vertebral arteries contained no labeled nerve fiber. In animals of which the ethmoidal nerve and the external ethmoidal artery were cut together with the surrounding periorbital soft tissues just before entering the ethmoidal foramen, no labeled nerve fiber was identified on the wall of the major cerebral arteries. Although labeled gangliocytes were found in the trigeminal and superior cervical ganglia after the tracer injection to the ipsilateral SPG, the chronic maxillary neurotomy and superior cervical ganglionectomy did not alter the distribution of the labeled nerve fibers on the wall of the cerebral arteries.(ABSTRACT TRUNCATED AT 250 WORDS)
Department of Neurosurgery, Shinshu University School of Medicine, Matsumoto, Japan.
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Hara
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Zhang
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Kuroyanagi
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Kobayashi
D000818:Animals; D001921:Brain / Q000098:blood supply*; D005726:Ganglia, Parasympathetic / Q000033:anatomy & histology*; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000294:innervation*; D009412:Nerve Fibers / Q000648:ultrastructure; D009474:Neurons / Q000648:ultrastructure; D010275:Parasympathetic Nervous System / Q000033:anatomy & histology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014664:Vasodilation / Q000502:physiology