Alteration in rat apolipoprotein C-III gene expression and lipoprotein composition during inflammation.
A cloned rat apolipoprotein (apo) C-III cDNA was used as a hybridization probe to measure apo C-III mRNA levels in rats after induction of inflammation. Hepatic apo C-III mRNA levels decrease to a minimum value of 46% of normal 72 h after the induction of inflammation. The changes observed are very similar to the changes in mRNA levels of rat apo A-IV, while the hepatic mRNA levels for apo A-I and apo C-I during inflammation remain relatively constant. Alterations in apo C-III gene expression during inflammation were associated with changes in lipoprotein particle size and composition. A decrease of approximately twofold in the amount of apo C-III-containing HDL particles was found in rats 24 h after the induction of inflammation. The average size of apo C-III-containing HDL particles in rats during inflammation is significantly larger than that of the control group. A decrease in the concentration of apo A-I-containing HDL particles was also observed in these rats. The results indicate altered apolipoprotein gene expression associated with alterations in the size and composition of HDL particles.
Shen
P
P
Russell Grimwade School of Biochemistry, University of Melbourne, Parkville, Victoria, Australia.
Howlett
G J
GJ
D000818:Animals; D053302:Apolipoprotein C-I; D053305:Apolipoprotein C-III; D001056:Apolipoproteins C / Q000097:blood / Q000737:chemistry* / Q000235:genetics*; D002850:Chromatography, Gel; D015870:Gene Expression; D007249:Inflammation / Q000097:blood / Q000378:metabolism*; D008055:Lipids / Q000097:blood; D008074:Lipoproteins / Q000097:blood / Q000737:chemistry*; D008075:Lipoproteins, HDL / Q000737:chemistry; D008297:Male; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011915:Rats, Inbred BUF
D053302:Apolipoprotein C-I; D053305:Apolipoprotein C-III; D001056:Apolipoproteins C; D008055:Lipids; D008074:Lipoproteins; D008075:Lipoproteins, HDL; D012333:RNA, Messenger