Induction of surface antigen recognized by new monoclonal antibody, YU311, on 1-beta-D-arabinofuranosylcytosine-resistant human leukemic cell line.
A new monoclonal antibody, YU311, against an antigen expressed on 1-beta-D-arabinofuranosylcytosine(ara-C)-resistant human leukemic cell line with decreased deoxycytidine kinase activity was generated. YU311 reacted with ara-C-resistant human leukemic cell line (KY-Ra), but not with its parental cell line (KY-821) which was sensitive to ara-C. YU311 recognized the 92-kDa membrane protein. Furthermore, YU311 inhibited the growth of KY-Ra in suspension medium with and without ara-C. In immunocytochemistry, there was no difference in expression of usual differentiation antigens between KY-Ra and KY-821. These findings indicate that antigenic change could occur in ara-C-resistant human leukemic cells with stable expression of differentiation antigens and that the 92-kDa membrane protein may be one of the membrane proteins which regulate cell growth of KY-Ra.
Kakihara
T
T
Department of Pediatrics, Niigata University School of Medicine, Japan.
Yamada
T
T
Fukuda
T
T
Ohnishi
Y
Y
Kishi
K
K
Uchiyama
M
M
Shibata
A
A
D000818:Animals; D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000032:analysis; D000954:Antigens, Surface / Q000032:analysis / Q000096:biosynthesis*; D002460:Cell Line; D003561:Cytarabine / Q000494:pharmacology*; D004351:Drug Resistance; D005434:Flow Cytometry; D015151:Immunoblotting; D016130:Immunophenotyping; D007938:Leukemia; D008565:Membrane Proteins / Q000032:analysis / Q000096:biosynthesis*; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000954:Antigens, Surface; D008565:Membrane Proteins; D003561:Cytarabine