The 0.7 to 3.3 megabase chromosomes from Candida, Kluyveromyces, and Pichia provide accurate size standards for pulsed field gel electrophoresis.
Pulsed field gel electrophoresis was used to size intact chromosomal DNAs from Candida albicans, Kluyveromyces lactis, Pichia scolyti, and Pichia mississippiensis by optimization methods using, as size standards, concatenated bacteriophage lambda DNA, and intact and NotI digestion products of Schizosaccharomyces pombe chromosomal DNAs. These newly sized fungal DNAs can now serve as convenient and accurate size standards for DNA molecules between 0.7 and 3.3 megabases (Mb). These size standards are valid over a wide range of different electrophoretic conditions.
Division of Structural Biology, Lawrence Berkeley Laboratory, Berkeley, CA.
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Zhang
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Fan
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Ringquist
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Smith
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Cantor
D002175:Candida / Q000235:genetics*; D004271:DNA, Fungal / Q000737:chemistry* / Q000302:isolation & purification; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods*; D005800:Genes, Fungal; D007716:Kluyveromyces / Q000235:genetics*; D010316:Particle Size; D010843:Pichia / Q000235:genetics*; D015183:Restriction Mapping
D004271:DNA, Fungal