The c-ets-1 proto-oncogenes in Xenopus laevis: expression during oogenesis and embryogenesis.
We previously reported the cloning and sequencing of two cDNAs derived from the Xenopus laevis ets-1 gene (Stiegler et al., 1990). The Xl-ets-1a cDNA encodes a polypeptide highly homologous to known ets-1 proteins. The 3'-UTR contains two AATAAA polyadenylation signals together with three copies of the TTTTTAT sequence thought to confer a maturation-specific polyadenylation and implicated in the deadenylation of dormant mRNAs. Several transcripts with maternal characteristics were detected in oogenesis and early embryogenesis. A marked augmentation of the major transcript in the poly(A)+ fraction was detected at fertilization. Ets-1 transcripts were observed at constant levels during the cleavage stages but decreased abruptly at gastrulation, to reappear from neurulation to late embryogenesis. The possible contribution of 3'-UTR sequence elements to this behavior is discussed.
Stiegler
P
P
UPR Mécanismes Moléculaires, Division Cellulaire et du Développement, CNRS, Strasbourg, France.
Wolff
C M
CM
Meyer
D
D
Sénan
F
F
Durliat
M
M
Hourdry
J
J
Befort
N
N
Remy
P
P
D000241:Adenosine / Q000235:genetics / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002645:Chickens; D005786:Gene Expression Regulation; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009024:Morphogenesis / Q000235:genetics; D009866:Oogenesis / Q000235:genetics; D011108:Polymers / Q000378:metabolism; D050784:Proto-Oncogene Protein c-ets-1; D011518:Proto-Oncogene Proteins / Q000737:chemistry / Q000235:genetics*; D050783:Proto-Oncogene Proteins c-ets; D011519:Proto-Oncogenes; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors; D014982:Xenopus laevis / Q000196:embryology / Q000235:genetics*
C494041:ETS1 protein, human; C494042:Ets1 protein, mouse; D011108:Polymers; D050784:Proto-Oncogene Protein c-ets-1; D011518:Proto-Oncogene Proteins; D050783:Proto-Oncogene Proteins c-ets; D014157:Transcription Factors; C069424:polyadenosine; D000241:Adenosine