bcl-2 inhibits death of central neural cells induced by multiple agents.
The protooncogene bcl-2, which has been implicated in B-cell lymphoma development, inhibits apoptosis due to growth factor withdrawal in some, but not all, hematopoietic cells. Recently we found that bcl-2 also inhibits apoptosis in PC12 pheochromocytoma cells. We now report that bcl-2 inhibits the death of a central neural cell line due to serum and growth factor withdrawal, the calcium ionophore A23187, glucose withdrawal, membrane peroxidation, and, in some cases, free radical-induced damage. This broad range of protective effects of BCL-2 protein suggests that BCL-2 may interact with a central step in neural cell death. Measurements of intracellular free calcium suggest that BCL-2 alters the transduction of neural death signals at a point distal to the rise in intracellular free calcium.
Zhong
L T
LT
Department of Neurology, University of California School of Medicine, Los Angeles 90024-1769.
Sarafian
T
T
Kane
D J
DJ
Charles
A C
AC
Mah
S P
SP
Edwards
R H
RH
Bredesen
D E
DE
D000818:Animals; D017209:Apoptosis; D002118:Calcium / Q000502:physiology; D016923:Cell Death; D015870:Gene Expression; D066298:In Vitro Techniques; D009474:Neurons / Q000166:cytology*; D016716:PC12 Cells; D011518:Proto-Oncogene Proteins / Q000502:physiology*; D019253:Proto-Oncogene Proteins c-bcl-2; D011994:Recombinant Proteins / Q000378:metabolism; D014162:Transfection
D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D011994:Recombinant Proteins; D002118:Calcium