Response to repeated inoculations with Ascaris suum eggs in pigs during the fattening period. II. Specific IgA, IgG, and IgM antibodies determined by enzyme-linked immunosorbent assay.
Experimental trickle inoculations of pigs with low doses (2 x 25 eggs/week) and high doses (2 x 500 eggs/week) of Ascaris suum were followed by enzyme-linked immunosorbent assay (ELISA) during a 14-week period. Three antigens were employed for coating: hatching fluid of embryonated eggs, excretory/secretory antigens from in vitro-cultivated infective larvae (L2/L3-ES), and adult body fluid. Seroconversion times (week 2 for the high-dose group and week 4 for the low-dose group) were essentially identical for the three antigens. The assay employing L2/L3-ES, however, produced significantly higher readings. Specific serum antibody in the IgA and IgG classes showed very similar time courses in both groups. A weak and transient, specific IgM response was recorded in the high-dose group. Mean ELISA responses to L2/L3-ES did not differ significantly between the groups at the termination of the experiment. Specific antibody correlated weakly with the number of liver milk spots recorded at slaughter.
Department of Veterinary Microbiology, Royal Veterinary and Agricultural University, Frederiksberg, Denmark.
P
P
Lind
L
L
Eriksen
P
P
Nansen
O
O
Nilsson
A
A
Roepstorff
D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood / Q000276:immunology*; D000918:Antibody Specificity; D001196:Ascariasis / Q000517:prevention & control / Q000662:veterinary*; D017165:Ascaris suum / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D007070:Immunoglobulin A / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D007075:Immunoglobulin M / Q000276:immunology; D013552:Swine; D013553:Swine Diseases / Q000469:parasitology / Q000517:prevention & control*; D013997:Time Factors; D014612:Vaccines / Q000276:immunology*
D000909:Antibodies, Helminth; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D014612:Vaccines