Rapid determination of a new angiotensin-converting enzyme inhibitor, imidapril, and its active metabolite in human plasma by negative-ion desorption chemical ionization-tandem mass spectrometry (MS/MS).
A very rapid and highly sensitive method using desorption chemical ionization (DCI)-tandem mass spectrometry (MS/MS) with selected reaction monitoring is reported for the simultaneous determination of imidapril and its active metabolite (M1) in human plasma. Imidapril and M1 in plasma were extracted by a C18 solid phase extraction cartridge after deproteinization, and derivatized with pentafluorobenzyl bromide. One microliter of prepared sample was applied to the DCI filament and analyzed by DCI/MS/MS within a few minutes. The limits of determination of imidapril and M1 were 0.2 and 0.5 ng/ml in human plasma, respectively. The features of this method make it appropriate for use in pharmacokinetic studies with human plasma after oral administration of imidapril.
Analytical Chemistry Research Laboratory, Tanabe Seiyaku Co., Ltd., Osaka, Japan.
S
S
Horimoto
M
M
Mabuchi
K
K
Banno
T
T
Sato
D000806:Angiotensin-Converting Enzyme Inhibitors / Q000097:blood*; D006801:Humans; D007093:Imidazoles / Q000097:blood*; D048289:Imidazolidines; D008297:Male; D013058:Mass Spectrometry / Q000379:methods; D015203:Reproducibility of Results
D000806:Angiotensin-Converting Enzyme Inhibitors; D007093:Imidazoles; D048289:Imidazolidines; C065166:imidapril