Cadaver lung donors: effect of preharvest ventilation on graft function.
The pulmonary donor pool would increase substantially if lungs could be safely transplanted after cessation of circulation. To determine whether ventilation of cadaver lungs could improve graft function, canine donors were sacrificed and then ventilated with 100% oxygen (n = 6) or 100% nitrogen (n = 6); 6 served as nonventilated controls. Four hours after death, the lungs were flushed with modified Euro-Collins solution and harvested. Controls were ventilated with 100% oxygen only during flush and harvest. Recipients were rendered dependent on the transplanted lung by occlusion of the right pulmonary artery and bronchus 1 hour after transplantation. Ventilation was maintained at a constant inspired oxygen fraction of 0.4. Four controls died of pulmonary edema shortly after occlusion of the native lung. The mean arterial oxygen tensions in the oxygen-ventilated, nitrogen-ventilated, and control groups at the end of 8 hours were 81 mm Hg (n = 4), 88 mm Hg (n = 3), and 55 mm Hg (n = 2), respectively. Postmortem oxygen ventilation improved early recipient survival and gas exchange. Postmortem nitrogen ventilation improved early gas exchange and delayed recipient death compared with non-ventilated controls. The mechanics of ventilation appears to confer a functional advantage independent of a continued supply of oxygen. Transplantation of lungs harvested from cadavers after cessation of circulation might be feasible.
Ulicny
K S
KS
Department of Surgery, University of North Carolina School of Medicine, Chapel Hill 27599-7065.
Egan
T M
TM
Lambert
C J
CJ
Reddick
R L
RL
Wilcox
B R
BR
D000818:Animals; D001794:Blood Pressure / Q000502:physiology; D002102:Cadaver; D002302:Cardiac Output / Q000502:physiology; D004285:Dogs; D015633:Extravascular Lung Water / Q000737:chemistry; D016040:Lung Transplantation / Q000379:methods* / Q000473:pathology / Q000502:physiology*; D009584:Nitrogen / Q000008:administration & dosage; D009926:Organ Preservation; D010100:Oxygen / Q000008:administration & dosage / Q000097:blood; D011175:Positive-Pressure Respiration; D011651:Pulmonary Artery / Q000503:physiopathology; D011659:Pulmonary Gas Exchange / Q000502:physiology; D015996:Survival Rate; D013990:Tidal Volume; D013997:Time Factors; D014655:Vascular Resistance / Q000502:physiology
D009584:Nitrogen; D010100:Oxygen