Molecular genetics in Saccharomyces kluyveri: the HIS3 homolog and its use as a selectable marker gene in S. kluyveri and Saccharomyces cerevisiae.
We cloned the Saccharomyces kluyveri HIS3 homolog, k-HIS3, and made a partial deletion of the gene. The k-HIS3 gene complemented a HIS3 deletion in S. cerevisiae. The DNA sequences of the open reading frames (ORFs) of the HIS3 homologs are 70% identical at the DNA level and 83% identical at the deduced amino acid level. The ORF upstream of the k-HIS3 gene is related to the PET56 gene of S. cerevisiae found upstream of the HIS3 gene of S. cerevisiae. The ORF downstream from the k-HIS3 gene is not related to the DED1 gene found downstream of the HIS3 gene in S. cerevisiae.
Laboratory of Eukaryotic Gene Expression, ABL-Basic Research Program, NCI-Frederick Cancer Research and Development Center, MD 21702-1201.
K G
KG
Weinstock
J N
JN
Strathern
D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D005800:Genes, Fungal / Q000235:genetics*; D005819:Genetic Markers; D006836:Hydro-Lyases / Q000235:genetics*; D008969:Molecular Sequence Data; D016296:Mutagenesis; D012440:Saccharomyces / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics; D012641:Selection, Genetic; D017422:Sequence Analysis, DNA; D014170:Transformation, Genetic; D014498:Uracil / Q000378:metabolism
D005819:Genetic Markers; D014498:Uracil; D006836:Hydro-Lyases; C021434:imidazoleglycerolphosphate dehydratase