A novel lectin in rabbit serum binds H type 1, H type 2 and N-acetyl lactosamine structures.
A novel lectin (RSL) which recognizes blood group H type 1 and type 2 (Fuc alpha 1-->2Gal beta 1-->3/4GlcNAc beta-R), and N-acetyllactosamine (Gal beta 1-->4GlcNAc beta-R) was purified from rabbit serum using affinity chromatography on Synsorb H type 2 beads, gel filtration and preparative polyacrylamide gel electrophoresis. The lectin agglutinated human O type red cells, and the hemagglutination reaction was inhibited by H type 1 and type 2 haptens, N-acetyllactosamine and human salivas from secretor individuals. The molecular weight of the lectin was estimated to be approximate 650,000 and 65,000 on Sephacryl S-400 gel filtration and SDS-polyacrylamide gel electrophoresis, respectively.
Hosomi
O
O
Department of Legal Medicine, School of Medicine, Gunma University, Maebashi, Japan.
Takeya
A
A
Yazawa
S
S
D000606:Amino Sugars / Q000378:metabolism*; D000818:Animals; D002240:Carbohydrate Sequence; D002478:Cells, Cultured; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D006241:Haptens / Q000145:classification / Q000378:metabolism*; D006385:Hemagglutination Inhibition Tests; D006386:Hemagglutination Tests; D006801:Humans; D037102:Lectins / Q000097:blood* / Q000302:isolation & purification / Q000378:metabolism; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011817:Rabbits
D000606:Amino Sugars; D006241:Haptens; D037102:Lectins; C000458:N-acetyllactosamine