The quantitative analysis of inhalational anaesthetics in forensic samples by gas chromatography/mass spectrometry/selected ion monitoring.
The quantitative analysis of volatile anaesthetics for biomedical applications by means of gas chromatograph/mass spectrometry/selected ion monitoring (GC/MS/SIM) was studied. Xenon gas was selected as an internal standard for the assay by adding to a closed system, because of its stability and inactivity. In the assay of inhalational anaesthetics, isoflurane and nitrous oxide (laughing gas), in forensic samples (serum and cerebrospinal fluid), the calibration of the anaesthetic was linear from 0.12 to 12 nmol/mL in isoflurane and from 30 to 300 nmol/mL in nitrous oxide. Our results suggest that this new method is suitable for the quantitative analysis of inhalational anaesthetics in the biomedical field.
Department of Legal Medicine, Jikei University School of Medicine, Tokyo, Japan.
K
K
Maruyama
A
A
Takatsu
T
T
Obata
D018685:Anesthetics, Inhalation / Q000032:analysis* / Q000097:blood / Q000134:cerebrospinal fluid; D001011:Aorta; D005554:Forensic Medicine; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods* / Q000706:statistics & numerical data; D006325:Heart Atria; D006801:Humans; D007530:Isoflurane / Q000032:analysis; D007700:Kinetics; D009609:Nitrous Oxide / Q000032:analysis; D014978:Xenon / Q000032:analysis
D018685:Anesthetics, Inhalation; D014978:Xenon; D007530:Isoflurane; D009609:Nitrous Oxide