Nerve growth factor antibodies recognize neurotrophin-3.
The immunological properties of the neurotrophins NGF, BDNF, and NT-3 were compared using polyclonal and monoclonal antibodies against the beta subunit of mouse NGF. Affinity-purified anti-NGF IgG consistently recognized NGF and NT-3 on Western blots, and inhibited the trophic activity of NGF and NT-3 but not BDNF. In contrast, anti-NGF monoclonal antibodies did not block the trophic activity of either NT-3 or BDNF. These results are consistent with the greater structural overlap between NGF and NT-3 than between NGF and BDNF.
Advanced Technology Division, Fidia S.P.A., Abano Terme, Italy.
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A
Negro
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V
Corsa
S D
SD
Skaper
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L
Callegaro
D000818:Animals; D000906:Antibodies / Q000276:immunology*; D000911:Antibodies, Monoclonal; D001483:Base Sequence; D015153:Blotting, Western; D051379:Mice; D008969:Molecular Sequence Data; D009414:Nerve Growth Factors / Q000276:immunology*; D009419:Nerve Tissue Proteins / Q000276:immunology*; D020933:Neurotrophin 3
D000906:Antibodies; D000911:Antibodies, Monoclonal; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins; D020933:Neurotrophin 3