Three small ribooligonucleotides with specific arginine sites.
Arginine-binding RNA motifs are important to protein-RNA interaction and perhaps also for Archean biochemistry. Selection-amplification was used to isolate three RNAs that are eluted by free arginine from an L-arginine affinity column (Kd approximately 0.2-0.4 mM). The binding sites contain specific internal and bulge loops, whose sequences can include arginine coding triplets. Binding is highly specific for arginine, but all three motifs, like the self-splicing group I intron, also bind guanosine 5'-monophosphate. One site is stereoselective, somewhat preferring D-arginine.
Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder 80309-0347.
G J
GJ
Connell
M
M
Illangesekare
M
M
Yarus
D001120:Arginine / Q000031:analogs & derivatives / Q000378:metabolism*; D001483:Base Sequence; D001665:Binding Sites; D007700:Kinetics; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009843:Oligoribonucleotides / Q000737:chemistry / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D013329:Structure-Activity Relationship
D009843:Oligoribonucleotides; D001120:Arginine