Changes in the concentration of ions during senescence of the human erythrocyte.
Flash frozen samples of normal human blood were cryosectioned and cryodried for electron probe x-ray microanalysis of the concentration of ions and elements in individual erythrocytes (RBCs). The data (expressed in mM/kg dry weight) demonstrated a systematic pattern of variation between the concentration of ions and elements in the RBCs. Specifically as K+ and Cl- decreased in concentration, Ca2+ and sulfur increased in concentration. Phosphorous, Na+ and Mg2+ did not demonstrate a significant pattern of change. These findings are related to the dehydration and to the volume decrease that accompanies senescence of the RBC.
Cameron
I L
IL
Department of Cellular and Structural Biology, University of Texas Health Science Center, San Antonio 78284.
Hardman
W E
WE
Smith
N K
NK
Fullerton
G D
GD
Miseta
A
A
D002118:Calcium / Q000032:analysis / Q000378:metabolism; D002712:Chlorides / Q000032:analysis / Q000378:metabolism; D004577:Electron Probe Microanalysis; D004905:Erythrocyte Aging / Q000502:physiology*; D004912:Erythrocytes / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D007477:Ions; D008274:Magnesium / Q000032:analysis / Q000378:metabolism; D010758:Phosphorus / Q000032:analysis / Q000378:metabolism; D011188:Potassium / Q000032:analysis / Q000378:metabolism; D012044:Regression Analysis; D012964:Sodium / Q000032:analysis / Q000378:metabolism; D013455:Sulfur / Q000032:analysis / Q000378:metabolism
D002712:Chlorides; D007477:Ions; D010758:Phosphorus; D013455:Sulfur; D012964:Sodium; D008274:Magnesium; D011188:Potassium; D002118:Calcium