Acrylamide induces immediate-early gene expression in rat brain.
Northern blot analysis was used to study the effects of acrylamide, a potent neurotoxin, on the induction of c-fos and c-jun mRNA in rat brain. Male Sprague-Dawley rats (10-12 weeks old) treated with acrylamide as a single dose (100 mg/kg, i.p.) or via drinking water (0.03% w/v) for 4 weeks, were used to study acute and chronic effects on immediate-early gene expression, respectively. Acute administration of acrylamide caused a statistically significant increase in the expression of c-fos (approx. 37%) and c-jun (approx. 17%) mRNA in rat brain. By contrast, the level of c-fos mRNA in chronic acrylamide treatment was not altered significantly, but the expression of c-jun mRNA was increased almost 100% as compared to control. These data show that the neurotoxin acrylamide induces immediate-early gene expression in the brain. The effects appear to be related to the route of administration, dose and duration of acrylamide treatment.
Gerontology Research Center, Laboratory of Biological Chemistry, Baltimore, MD.
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Pekala
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D020106:Acrylamide; D000178:Acrylamides / Q000494:pharmacology*; D000818:Animals; D001345:Autoradiography; D015152:Blotting, Northern; D001923:Brain Chemistry / Q000187:drug effects*; D015870:Gene Expression / Q000187:drug effects*; D016762:Genes, fos / Q000187:drug effects*; D016758:Genes, jun / Q000187:drug effects*; D008297:Male; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000302:isolation & purification / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
D000178:Acrylamides; D012333:RNA, Messenger; D020106:Acrylamide