Protective immunity and mast cell and eosinophil responses in mice infested with larval Haemaphysalis longicornis ticks.
WBB6F1-+/+ mice were infested with larval Haemaphysalis longicornis ticks twice at an interval of 14 days: apparent resistance against ticks was expressed in the second infestation. The first infestation induced degranulation of a small number of mast cells at the feeding sites within 6 days, and resulted in two-fold increases of mast cell numbers on day 14 with a significant elevation of total immunoglobulin E (IgE) levels in sera and high proportion of IgE-bound mast cells. The second infestation resulted in the intensive degranulation of the increased mast cells at the feeding sites. Eosinophils infiltrated into the feeding sites of ticks: the second infestation led to a greater maximal level of the infiltrating eosinophils. These data suggest that the resistance against larval H. longicornis ticks in mice may be expressed as a result of immediate hypersensitivity and many eosinophils infiltrating from the blood to the feeding sites might contribute to the tick rejection.
Ushio
H
H
Department of Veterinary Surgery, College of Agriculture, University of Osaka Prefecture, Japan.
Watanabe
N
N
Kiso
Y
Y
Higuchi
S
S
Matsuda
H
H
D000818:Animals; D002452:Cell Count; D015550:Cell Degranulation; D004804:Eosinophils / Q000276:immunology*; D006969:Hypersensitivity, Immediate / Q000276:immunology; D007110:Immunity, Active; D007073:Immunoglobulin E / Q000096:biosynthesis; D007814:Larva / Q000276:immunology; D007958:Leukocyte Count; D008297:Male; D008407:Mast Cells / Q000276:immunology* / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D010323:Passive Cutaneous Anaphylaxis / Q000276:immunology; D012867:Skin / Q000276:immunology / Q000473:pathology; D013047:Specific Pathogen-Free Organisms; D013984:Tick Infestations / Q000276:immunology* / Q000473:pathology; D013987:Ticks / Q000276:immunology*; D013997:Time Factors
D007073:Immunoglobulin E