Signal processing in slit-scan flow cytometry of cell conjugates.
The design and implementation of a real-time signal processing system for slit-scan flow cytometry is described. The system is used to measure the separate scatter and fluorescence peak heights of 2 adherent cells. Preliminary measurements of changes in the membrane potential induced by interactions between natural killer (NK) cells and their target cells are presented.
Bakker Schut
T C
TC
Department of Applied Physics, University of Twente, Enschede, The Netherlands.
Florians
A
A
Radosević
K
K
de Grooth
B G
BG
Greve
J
J
D001463:Barbiturates; D002448:Cell Adhesion; D003602:Cytotoxicity, Immunologic; D004581:Electronics / Q000295:instrumentation; D005434:Flow Cytometry / Q000295:instrumentation*; D005453:Fluorescence; D006801:Humans; D007555:Isoxazoles; D007694:Killer Cells, Natural / Q000166:cytology*; D004915:Leukemia, Erythroblastic, Acute; D008564:Membrane Potentials; D012542:Scattering, Radiation; D014407:Tumor Cells, Cultured
D001463:Barbiturates; D007555:Isoxazoles; C052795:bis(1,3-dibutylbarbiturate)trimethine oxonol