Expanding the capabilities of laboratory instruments using built-in microprocessors and serial interfaces: adapting an LKB Ultrospec II UV spectrophotometer for scanning and enzyme kinetic analyses.
An IBM-compatible microcomputer was used to enable an LKB Ultrospec II UV-Visible spectrophotometer to perform two features which are not available on the basic unit: wavelength scanning and enzyme kinetic analyses. The spectrophotometer was controlled using a simple BASIC program, an RS232c interface and the instrument's own built-in microprocessor. This technique required no special analogue to digital conversion hardware or software and only a rudimentary knowledge of programming. User-designed software permits a greater degree of flexibility in routines and output formats than is offered in most pre-programmed instruments, while offering substantial savings.
Department of Oceanography, University of British Columbia, Vancouver, Canada.
J A
JA
Berges
C
C
Virtanen
D003626:Data Display; D004798:Enzymes / Q000032:analysis / Q000737:chemistry; D007770:L-Lactate Dehydrogenase / Q000737:chemistry; D007773:Lactates / Q000032:analysis / Q000737:chemistry; D008838:Microcomputers; D009243:NAD / Q000032:analysis / Q000737:chemistry; D010084:Oxidation-Reduction; D011381:Programming Languages; D011773:Pyruvates / Q000032:analysis / Q000737:chemistry; D012984:Software; D013056:Spectrophotometry, Ultraviolet / Q000295:instrumentation*
D004798:Enzymes; D007773:Lactates; D011773:Pyruvates; D009243:NAD; D007770:L-Lactate Dehydrogenase