Alpha 1-trypsin immunoreactivity in Alzheimer disease.
This study is the initial demonstration of trypsin-like immunoreactivity within the human CNS. Using two monoclonal antibodies to alpha 1-trypsin, we found trypsin-like immunoreactivity associated with the senile plaques of Alzheimer disease. Immunoblots prepared from homogenized Alzheimer and control cortex showed increased amounts of the 24,000Da alpha 1-trypsin species in diseased compared to control tissue. We suggest an alteration in alpha 1-trypsin protease regulation could be important in the formation of senile plaques and the pathogenesis of Alzheimer disease.
Department of Pathology, Case Western Reserve University, Cleveland, OH 44106-4901.
M A
MA
Smith
R N
RN
Kalaria
G
G
Perry
D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000201:enzymology* / Q000473:pathology; D000911:Antibodies, Monoclonal; D002540:Cerebral Cortex / Q000201:enzymology* / Q000473:pathology; D006624:Hippocampus / Q000201:enzymology* / Q000473:pathology; D006801:Humans; D015151:Immunoblotting; D007150:Immunohistochemistry; D008875:Middle Aged; D008970:Molecular Weight; D012016:Reference Values; D014357:Trypsin / Q000032:analysis*
D000911:Antibodies, Monoclonal; D014357:Trypsin