Immunoaffinity chromatography combined with gas chromatography-negative ion chemical ionisation mass spectrometry for the confirmation of flumethasone abuse in the equine.
Immunoaffinity chromatography using a synthesised immunosorbent was used to extract tritiated dexamethasone (with dexamethasone carrier) from equine urine at a recovery of 81.7 +/- 8.4% (mean +/- S.D.). A method utilising this procedure coupled to cool on-column injection gas chromatography-negative ion chemical ionisation mass spectrometry is also described for the confirmation of low levels of flumethasone in equine urine samples.
Laboratory of the Jockey Club of Southern Africa, Johannesburg.
S M
SM
Stanley
B S
BS
Wilhelmi
J P
JP
Rodgers
H
H
Bertschinger
D000818:Animals; D000906:Antibodies; D002846:Chromatography, Affinity / Q000379:methods*; D003429:Cross Reactions; D003907:Dexamethasone; D004300:Doping in Sports; D005443:Flumethasone / Q000652:urine*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006736:Horses; D006854:Hydrocortisone; D015203:Reproducibility of Results
D000906:Antibodies; D003907:Dexamethasone; D005443:Flumethasone; D006854:Hydrocortisone