Isolation of a serotype G6P11 bovine rotavirus showing two-way cross-neutralization with the serotype G10P11 virus.
Four stains designated as OB94-1 to OB94-4 of group A bovine rotavirus (BRV) were isolated from 35 fecal samples of calves with diarrhea in sporadic outbreaks. In VP7 (G) and VP4 (P) serotyping of these isolates, OB94-1 to OB94-3 were determined as G6P5, G6P5 and G10P5, respectively, by cross neutralization (NT) test and the G- and P- serotyping polymerase chain reaction (PCR) analysis. OB94-4 showed a one-way antigenic relation with the Lincoln stain (G6P1) and a weak antigenic relationship with the KK3 strain (G10P11), and was determined as G6P11 by the PCR method. Thus, OB94-4 was shown to be a new G6 BRV with different antigenic properties from the others in the NT test.
Fukutomi
T
T
Okayama Prefectural Veterinary Diagnostic Laboratory, Japan.
Fujiwara
M
M
Sanekata
T
T
Akashi
H
H
D000818:Animals; D000914:Antibodies, Viral; D000956:Antigens, Viral / Q000032:analysis; D002417:Cattle; D002418:Cattle Diseases; D003967:Diarrhea / Q000662:veterinary* / Q000821:virology; D004196:Disease Outbreaks / Q000662:veterinary; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005243:Feces / Q000821:virology; D009500:Neutralization Tests; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary; D012401:Rotavirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D012400:Rotavirus Infections / Q000662:veterinary*; D012703:Serotyping; D013045:Species Specificity
D000914:Antibodies, Viral; D000956:Antigens, Viral