Cloning of the mouse interleukin 2 receptor gamma chain: demonstration of functional differences between the mouse and human receptors.
We isolated a cDNA clone for the gamma chain of the mouse interleukin 2 receptor. Introduction of the mouse gamma chain cDNA clone into a mouse fibroblast cell line, L929, expressing the mouse alpha beta heterodimer IL-2 receptor converted pseudo-high affinity of the IL-2 receptor into functional high, resulting in internalization of IL-2 and induction of the c-myc, c-fos and c-jun genes. The mouse beta gamma heterodimer, however, failed to bind IL-2 unlike the human beta gamma heterodimer intermediate-affinity receptor. These results indicate that the mouse functional IL-2 receptor is a complex comprising three distinct subunits, alpha, beta and gamma chains, but the beta gamma heterodimer is not functional and different from the human heterodimer.
Department of Microbiology, Tohoku University School of Medicine, Sendai, Japan.
S
S
Kumaki
M
M
Kondo
T
T
Takeshita
H
H
Asao
M
M
Nakamura
K
K
Sugamura
D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D003001:Cloning, Molecular; D004247:DNA; D006801:Humans; D007376:Interleukin-2 / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D015375:Receptors, Interleukin-2 / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
D007376:Interleukin-2; D015375:Receptors, Interleukin-2; D004247:DNA