The S-100 protein causes an increase of intracellular calcium and death of PC12 cells.
The S-100 protein-PC12 cell interaction has been studied as a model system of the possible physiological role played by S-100 protein in the nervous system. The data reported demonstrate that S-100 exerts a cytotoxic action which eventually leads to PC12 cell death, regardless of the cell cycle phase. The effect is specific for the S-100 isoforms, which are made up of two identical subunits and is abolished by a monoclonal antibody directed against the same isoforms. Other isoforms and/or calcium-binding proteins, such as troponin or calmodulin, do not induce the same effects. The action of S-100 on cell viability is not detectable in other cell lines of different embryological origin, such as 3T3, L1210, GH3. S-100 causes a rapid and considerable increase (two- to three-fold) of intracellular Ca2+ concentration in PC12 cells accompanied by cytostatic and cytotoxic action. It is postulated that this action also occurs in vivo, as part of the physiological action of this protein.
Fanò
G
G
Istituto di Biologia Cellulare, Università di Perugia, Italy.
Mariggiò
M A
MA
Angelella
P
P
Nicoletti
I
I
Antonica
A
A
Fulle
S
S
Calissano
P
P
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002118:Calcium / Q000378:metabolism*; D002132:Calcium Radioisotopes; D002135:Calcium-Binding Proteins / Q000378:metabolism; D016923:Cell Death / Q000187:drug effects; D009414:Nerve Growth Factors / Q000494:pharmacology; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D016716:PC12 Cells; D011485:Protein Binding; D051381:Rats; D009418:S100 Proteins / Q000633:toxicity*
D000911:Antibodies, Monoclonal; D002132:Calcium Radioisotopes; D002135:Calcium-Binding Proteins; D009414:Nerve Growth Factors; D009418:S100 Proteins; D002118:Calcium