Lactate oxidation for the detection of mitochondrial dysfunction in human skin fibroblasts.
To screen fibroblasts for defects in lactate/pyruvate oxidation, cells were grown to confluence in 25-cm2 flasks, rinsed, and incubated in glucose-free media containing 25 microM L-lactate and 0.1 microCi [D,L-1-14C]lactate. Lactate oxidation was measured as the amount of lactate oxidized in nmol of 14CO2 generated/mg protein/min. Fibroblasts from patients with mitochondrial or peroxisomal disorders had decreased lactate oxidation compared to the control (CON): CON, 1.9 +/- 0.13 nmol/mg/min; neonatal adrenoleukodystrophy (NALD), 0.45 +/- 0.01 (P < 0.001); rhizomelic chondrodysplasia punctata (RCDP), 0.13 +/- 0.002 (P < 0.001); mitochondrial defect of unknown etiology (MIT), 0.77 +/- 0.003 (P < 0.001); pyruvate dehydrogenase (PDH) deficiency, 0.98 +/- 0.02 (P < 0.001). This method is useful for screening fibroblasts for defects in lactate oxidation in patients with mitochondrial or peroxisomal disorders. Confirmation of the site of the defect may then be investigated with specific assays, e.g., PDH, in cellular homogenates: CON, 0.93 +/- 0.02 nmol/mg/min; NALD, 0.55 +/- 0.02; RCDP, 0.44 +/- 0.02; MIT, 0.53 +/- 0.03; PDH deficiency, 0.19 +/- 0.02.
William Beaumont Hospital, Royal Oak, Michigan 48073.
J P
JP
Ofenstein
F L
FL
Kiechle
D M
DM
Dandurand
W M
WM
Belknap
K H
KH
Moore
R D
RD
Holmes
D000140:Acidosis, Lactic / Q000201:enzymology / Q000378:metabolism / Q000503:physiopathology; D002452:Cell Count; D003470:Culture Media; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D007773:Lactates / Q000378:metabolism*; D008830:Microbodies / Q000201:enzymology / Q000378:metabolism / Q000502:physiology; D008928:Mitochondria / Q000378:metabolism / Q000502:physiology*; D017240:Mitochondrial Myopathies / Q000175:diagnosis / Q000378:metabolism*; D010084:Oxidation-Reduction; D011768:Pyruvate Dehydrogenase Complex / Q000378:metabolism; D011773:Pyruvates / Q000378:metabolism; D012867:Skin / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure
D003470:Culture Media; D007773:Lactates; D011768:Pyruvate Dehydrogenase Complex; D011773:Pyruvates; D007770:L-Lactate Dehydrogenase