Signal processing in slit-scan flow cytometry of cell conjugates.
The design and implementation of a real-time signal processing system for slit-scan flow cytometry is described. The system is used to measure the separate scatter and fluorescence peak heights of 2 adherent cells. Preliminary measurements of changes in the membrane potential induced by interactions between natural killer (NK) cells and their target cells are presented.
Department of Applied Physics, University of Twente, Enschede, The Netherlands.
T C
TC
Bakker Schut
A
A
Florians
K
K
Radosević
B G
BG
de Grooth
J
J
Greve
D001463:Barbiturates; D002448:Cell Adhesion; D003602:Cytotoxicity, Immunologic; D004581:Electronics / Q000295:instrumentation; D005434:Flow Cytometry / Q000295:instrumentation*; D005453:Fluorescence; D006801:Humans; D007555:Isoxazoles; D007694:Killer Cells, Natural / Q000166:cytology*; D004915:Leukemia, Erythroblastic, Acute; D008564:Membrane Potentials; D012542:Scattering, Radiation; D014407:Tumor Cells, Cultured
D001463:Barbiturates; D007555:Isoxazoles; C052795:bis(1,3-dibutylbarbiturate)trimethine oxonol