Isolation and purification of proteoglycans.
Purification of a protein typically involves development of a quantitative assay to track protein integrity (e.g. enzyme activity) during subsequent isolation steps. The generalized procedure involves choosing the source of the protein, defining extraction conditions, developing bulk purification methods followed by refined, more selective methods. The purification of proteoglycans is often complicated by a) limited source quantities, b) necessity of chaotropic solvents for efficient extraction, c) their large molecular size and d) lack of defined functions to enable purity (i.e. activity, conformation) to be assessed. Because the usual goal of proteoglycan purification is physical characterization (intact molecular weight, core protein and glycosaminoglycan class and size), the problems of a suitable assay and/or native conformation are avoided. The 'assay' for tracking proteoglycan isolation typically utilizes uronic acid content or radiolabel incorporation as a marker. Once extracted from their cellular/extracellular environment, proteoglycans can be isolated by density gradient centrifugation and/or column chromatography techniques. Recent advances in the composition of chromatographic supports have enabled the application of ion-exchange, gel permeation, hydrophobic interaction and affinity chromatography resins using efficient high-pressure liquid chromatography to proteoglycan purification.
Fedarko
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Bone Research Branch, National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland 20892.
D002845:Chromatography / Q000379:methods; D004586:Electrophoresis; D006025:Glycosaminoglycans / Q000302:isolation & purification; D006595:Hexosamines / Q000032:analysis; D006801:Humans; D011509:Proteoglycans / Q000302:isolation & purification*; D013056:Spectrophotometry, Ultraviolet; D014574:Uronic Acids / Q000032:analysis
D006025:Glycosaminoglycans; D006595:Hexosamines; D011509:Proteoglycans; D014574:Uronic Acids