Chromosome analysis of human spermatozoa stored in vitro.
Two-hundred-sixty-five chromosome spreads from control human sperm samples capacitated in TEST-yolk buffer at 4 degrees C and 232 chromosome spreads from sperm samples incubated in vitro in Biggers-Whitten-Whittingham (BWW) for 24 h at room temperature prior to capacitation, were studied after fusion of sperm cells with zona-free hamster eggs. Sperm cells were provided by two volunteer donors. The results indicate an increase in chromosome structural abnormalities after in-vitro incubation of the spermatozoa from 1.8 to 7.7% in donor no. 1 and from 4.5 to 12.5% in donor no. 7. Overall, structural abnormalities increased 3.3-fold. The number of aneuploid spermatozoa and the sex ratio did not change significantly. The implications of the use of different media for storing spermatozoa are discussed.
Department of Medical Genetics, West Penn Hospital, Pittsburgh, PA 15224.
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D000328:Adult; D002869:Chromosome Aberrations / Q000209:etiology*; D025063:Chromosome Disorders; D005260:Female; D006801:Humans; D007621:Karyotyping; D008297:Male; D012662:Semen Preservation / Q000379:methods*; D013094:Spermatozoa / Q000502:physiology*