DNA strand breaks in cells with DNA repair deficiency after halothane exposure in vitro.
Halothane (CAS 151-67-7) induced DNA strand breaks in isolated lymphocytes of two patients with a deficient DNA repair (xeroderma pigmentosum). In lymphocytes (resting cells) of healthy human donors and in L 5178 Y cells (proliferating cells) of mouse lymphoma, halothane did not induce demonstrable DNA strand breaks. The cells were exposed to 1.0 vol/% halothane for 60 min, and the DNA strand breaks were demonstrated by alkaline elution. The results suggest a possible genotoxic side effect of halothane in patients with deficiency in DNA repair.
Department of Physiological Chemistry, University of Mainz (Fed. Rep. of Germany.
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M
Reitz
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Lanz
D000328:Adult; D000818:Animals; D004249:DNA Damage; D004260:DNA Repair / Q000187:drug effects*; D005260:Female; D006221:Halothane / Q000633:toxicity*; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects; D008223:Lymphoma / Q000378:metabolism; D008297:Male; D051379:Mice; D014407:Tumor Cells, Cultured; D014983:Xeroderma Pigmentosum / Q000378:metabolism
D006221:Halothane