A 54-kDa protein overexpressed by chloroquine-resistant Plasmodium berghei ANKA strain.
Using an insoluble chloroquine-adsorbent, a 54-kDa protein (with a range of 50-60 kDa) was extracted from serum of mice infected with chloroquine-resistant (CR) Plasmodium berghei ANKA strain. Immunoblotting assay with antiserum against the 54-kDa protein showed that the content of the protein was higher in serum of mice infected with the CR parasites than that of mice infected with chloroquine-sensitive (CS) P berghei ANKA strain, and that instead of the 54-kDa protein, a set of 15-, 16-, and 23-kDa proteins was found to be highly overexpressed in lysate of purified CR parasites in comparison with that of purified CS parasites, suggesting the 54-kDa protein probably to be composed of 3 subunits. These findings may bear great importance in probing mechanism of chloroquine resistance in malaria parasites.
Department of Parasitology, Second Military Medical University, Shanghai, China.
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D000818:Animals; D002738:Chloroquine / Q000494:pharmacology; D004351:Drug Resistance; D007106:Immune Sera; D015151:Immunoblotting; D008288:Malaria / Q000097:blood*; D008297:Male; D051379:Mice; D010962:Plasmodium berghei / Q000737:chemistry* / Q000145:classification / Q000187:drug effects; D015800:Protozoan Proteins / Q000032:analysis* / Q000276:immunology
D007106:Immune Sera; D015800:Protozoan Proteins; D002738:Chloroquine