Sequence determination of the P gene of simian virus 41: presence of irregular deletions near the RNA-editing sites of paramyxoviruses.
The complete nucleotide sequence of the P gene of simian virus 41 (SV41) was determined. The gene was found to be 1406 nucleotides long and to contain a relatively small open reading frame encoding a cysteine-rich V protein with a calculated M(r) of 24076. We have demonstrated that RNA-editing events occur in SV41 P gene transcripts and that the ratio of edited mRNAs to faithfully copied mRNA (P-mRNA:V-mRNA) is about 1:5 at either 24 or 40 h post-infection. The mRNA with two G insertions was capable of encoding a P protein of 395 amino acids with a predicted M(r) of 41,992. A kinetic study of P and V proteins by Western blot analysis showed that in virus-infected cells the amounts of both proteins were almost equal although the V-mRNA was considerably more abundant than the P-mRNA. Alignment of the SV41 P and V proteins with those of nine other paramyxoviruses demonstrated that irregular gaps were present around the RNA-editing sites.
Department of Microbiology, Mie University School of Medicine, Japan.
M
M
Kawano
M
M
Tsurudome
N
N
Oki
M
M
Nishio
H
H
Komada
H
H
Matsumura
S
S
Kusagawa
H
H
Ohta
Y
Y
Ito
D000595:Amino Acid Sequence; D001483:Base Sequence; D015153:Blotting, Western; D004279:DNA, Viral; D005814:Genes, Viral; D008969:Molecular Sequence Data; D010252:Paramyxoviridae / Q000145:classification / Q000235:genetics*; D010802:Phylogeny; D017393:RNA Editing; D012367:RNA, Viral; D017384:Sequence Deletion; D017386:Sequence Homology, Amino Acid; D014764:Viral Proteins / Q000235:genetics*
D004279:DNA, Viral; C081133:P protein, Simian parainfluenza virus 41; D012367:RNA, Viral; D014764:Viral Proteins