Precise sex selected births of mice following single cell embryo biopsy and Y-linked testis-specific gene analysis.
We report on the birth of 39 mice following single cell embryo biopsy and precise sex determination following in-vitro fertilization. Polymerase chain reaction was used to amplify fragments of the mouse testis-specific gene sequence (pYMT2/B) on the Y chromosome and the ovary-specific gene (ZP3) sequence on chromosome 5 from the single biopsied cell. Embryo biopsy was not associated with any deleterious effects.
Cui
K H
KH
Department of Obstetrics and Gynaecology, Queen Elizabeth Hospital, Woodville, S.A., Australia.
Putland
R A
RA
Seamark
R F
RF
Matthews
C D
CD
D000818:Animals; D001483:Base Sequence; D001706:Biopsy; D008040:Genetic Linkage; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D015203:Reproducibility of Results; D012743:Sex Preselection / Q000379:methods*; D013737:Testis / Q000502:physiology*; D014998:Y Chromosome; D015053:Zygote / Q000502:physiology*