CD and NMR studies on the aggregation of amphotericin-B in solution.
We report in this paper the aggregation properties of amphotericin-B (amp-B) in solution using CD and 1H-NMR techniques. Our results indicate that the preferred structure of amp-B in dimethylsulfoxide is a monomer at low concentrations (10(-4) M and below) and a stable dimer at higher concentrations (range 5.10(-3) M to 10(-2) M). In a DMSO/ethanol mixture (1:1 (v/v)), the antibiotic is monomeric, irrespective of the concentration within the range studied. We propose a head-to-tail model based on NMR data. An understanding of the head-to-tail dimer, is, we believe important, particularly in view of the recent report wherein it is proposed that the drug inserts into bilayers as head-to-tail oligomers.
Balakrishnan
A R
AR
Molecular Biophysics Unit, Indian Institute of Science, Bangalore.
Easwaran
K R
KR
D000666:Amphotericin B / Q000737:chemistry*; D002942:Circular Dichroism; D003460:Crystallization; D009682:Magnetic Resonance Spectroscopy; D008968:Molecular Conformation
D000666:Amphotericin B