A hyperthermophilic sulfur-reducing archaebacterium, Thermococcus sp. DT1331, isolated from a deep-sea hydrothermal vent.
A hyperthermophilic archaebacterium was isolated from a deep-sea black smoker chimney (depth, 760 m) at the Minami-ensei Knoll (28 degrees 23'N, 127 degrees 38'E). The strain, designated DT1331, was a coccoid shaped bacterium about 0.5 to 1.0 microns in diameter. The cells were surrounded by a cell envelope. The temperature for growth was between 55 degrees C and 93 degrees C with an optimum 80 degrees C. The growth occurred from pH 4.5 to 8.5 and the optimum pH was 6.0. DT1331 required 1% to 5% NaCl for growth and cell lysis was observed below 1% NaCl concentration. The strain was an anaerobic chemoorganotroph requiring elemental sulfur obligately. Organic substrates used included tryptone, peptone, soytone, casein, gelatin, and yeast extract. Under the optimal conditions, DT1331 had a generation time of 50 min and could reach densities of about 1.5 x 10(8) cells/ml. DT1331 was resistant to ampicillin, chloramphenicol, erythromycin, kanamycin, streptomycin, and tetracycline, which was one of the common characteristics of archaebacteria. The G+C content of DT1331 was 52.3 mol%. Analysis of the 16S rRNA gene by restriction enzymes coincided with those of Thermococcus celer, indicating that this strain belonged to the genus Thermococcus.
Korea Ginseng and Tobacco Research Institute, Taejon.
Y S
YS
Kwak
T
T
Kobayashi
T
T
Akiba
K
K
Horikoshi
Y B
YB
Kim
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001105:Archaea / Q000166:cytology / Q000302:isolation & purification / Q000502:physiology*; D001483:Base Sequence; D002455:Cell Division; D003470:Culture Media; D004247:DNA / Q000737:chemistry; D004352:Drug Resistance, Microbial; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D009792:Oceans and Seas; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D015183:Restriction Mapping; D012623:Seawater; D012965:Sodium Chloride / Q000032:analysis; D013696:Temperature
D000900:Anti-Bacterial Agents; D003470:Culture Media; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride; D004247:DNA