Construction of an infectious DNA clone of the Y1 strain of canine parvovirus and characterization of the virus derived from the clone.
We have cloned genome fragments of canine parvovirus strain Y1 from replicative-form DNA and double-stranded DNA synthesized from virion DNA in vitro, and constructed a recombinant plasmid containing a full-length Y1 genome (pCPVY 1). When this recombinant plasmid was transfected into cell cultures, an infectious virus could be recovered. To characterize this pCPVY 1-derived virus, its biological properties were compared with those of the parental strain. No difference was observed between them in antigen expression, viral DNA replication, hemagglutination ability, and virus multiplication, indicating that the virus derived from the infectious plasmid inherited the biological properties of the authentic Y1 strain. Therefore, this recombinant plasmid appears to be useful for reverse genetics of canine parvovirus.
Department of Veterinary Public Health, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan.
M
M
Horiuchi
M
M
Shinagawa
D000818:Animals; D002415:Cats; D002478:Cells, Cultured; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000235:genetics*; D004285:Dogs / Q000382:microbiology; D016679:Genome, Viral; D006386:Hemagglutination Tests; D015151:Immunoblotting; D010321:Parvoviridae / Q000235:genetics* / Q000502:physiology; D010957:Plasmids; D010948:Viral Plaque Assay
D004279:DNA, Viral