High-performance liquid chromatography of phosphatidic acid.
This paper reviews existing high-performance liquid chromatographic (HPLC) methods for the analysis of phosphatidic acid (PA) in various sample matrices. In addition to the introductory background discussion on important aspects of PA in lipid biochemistry, the review provides comprehensive coverage in the areas of derivatization techniques, detection methods, and HPLC separation techniques. Conversions of PA to suitable derivatives enhance the detection sensitivity and improve the chromatographic behavior of the analytes. Detection methods include the use of state-of-the-art detectors and are discussed in terms of sensitivity, specificity, and compatibility with analytical systems. Pertinent normal-phase and reversed-phase HPLC data for PA are compiled from published methods.
Abidi
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U.S. Department of Agriculture, National Center for Agricultural Utilization Research, Peoria, IL 61604, USA.
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D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D010712:Phosphatidic Acids / Q000032:analysis*; D013057:Spectrum Analysis
D010712:Phosphatidic Acids