In vitro granuloma formation by spleen cells treated with psoralen plus long-wave ultraviolet radiation.
In vitro granulomas are induced by culturing murine spleen cells with artificial microparticles, dextran beads. In the presence of 0.5 microgram/mL 8-methoxypsoralen, UVA radiation (0.2-2.0 J/cm2) suppressed granuloma formation in a UVA dose-dependent manner. The doses of PUVA did not affect the cell viability as assessed by trypan blue exclusion. The time course of granuloma formation in 0.5 J/cm2 PUVA-treated cells was similar to that of normal spleen cells, with a maximum granuloma index at day 3 of culture, although a 49-63% suppression of granuloma formation was observed. PUVA-treated, nonadherent cells produced the same granuloma index when cultured with normal adherent cells. In contrast, a smaller granuloma index was observed in PUVA-treated adherent cells even when they were cultured with normal nonadherent cells. These data suggest that PUVA alters macrophages, resulting in the suppression of granuloma formation in vitro.
Okamoto
H
H
Department of Dermatology, Faculty of Medicine, Kyoto University, Japan.
Guo
Z
Z
Imamura
S
S
D000818:Animals; D002448:Cell Adhesion; D002478:Cells, Cultured; D005260:Female; D006099:Granuloma / Q000209:etiology* / Q000473:pathology; D008730:Methoxsalen / Q000633:toxicity*; D051379:Mice; D008809:Mice, Inbred C3H; D011701:PUVA Therapy; D011833:Radiation Injuries, Experimental; D013154:Spleen / Q000187:drug effects* / Q000473:pathology / Q000528:radiation effects*; D013158:Splenic Diseases / Q000209:etiology / Q000473:pathology; D014466:Ultraviolet Rays
D008730:Methoxsalen