Polyclonal antibody-based immunoradiometric assay for quantification of cholesteryl ester transfer protein.
Cholesteryl ester transfer protein (CETP) catalyzes the transfer of neutral lipids among plasma lipoproteins and in this way plays a prominent role in cholesterol metabolic routing and, thus, probably for atherosclerosis. Studies of this important protein in various clinical settings require the ability to accurately quantify CETP in plasma. In order to gain access to such a capability, an immunoradiometric assay (IRMA) for quantification of CETP was developed. CETP was purified from human plasma to apparent homogeneity and used for raising anti-CETP antibodies in rabbits. The specificity of the polyclonal antiserum obtained was demonstrated by inhibition assays and immunoblot analysis. Before use in the CETP-IRMA, the antibodies were affinity-purified by chromatography on CETP-Sepharose. Sensitivity of the CETP-IRMA was 0.1 ng, and intra- and interassay coefficients of variation were 2.9 and 8.0%, respectively. In 30 normolipidemic healthy subjects, the mean (+/- SD) CETP concentration was 1.1 (+/- 0.22) micrograms/ml of plasma; individual values ranged from 0.644 to 1.694 micrograms CETP/ml and agreed well with measurements of CETP activity of the same samples (r = 0.85).
Ritsch
A
A
Department of Medicine, University of Innsbruck, Austria.
Auer
B
B
Föger
B
B
Schwarz
S
S
Patsch
J R
JR
D000906:Antibodies; D002352:Carrier Proteins / Q000097:blood* / Q000302:isolation & purification; D053480:Cholesterol Ester Transfer Proteins; D006023:Glycoproteins; D006801:Humans; D007074:Immunoglobulin G; D015592:Immunoradiometric Assay / Q000379:methods*; D007553:Isotope Labeling; D008075:Lipoproteins, HDL / Q000302:isolation & purification; D008077:Lipoproteins, LDL / Q000302:isolation & purification
D000906:Antibodies; C506642:CETP protein, human; D002352:Carrier Proteins; D053480:Cholesterol Ester Transfer Proteins; D006023:Glycoproteins; D007074:Immunoglobulin G; D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL