The 5' flanking sequences of the mouse P-cadherin gene. Homologies to 5' sequences of the E-cadherin gene and identification of a first 215 base-pair intron.
A genomic clone containing the 5' region of the mouse P-cadherin gene has been isolated from Balb/c mice. A major feature of this genomic sequence is the presence of a first intron (Il), 215 bp long, located 48 bp downstream of the translation start ATG codon. The presence of Il has been detected both in Balb/c and C57BL/6 mouse strains, being located at the same position with respect to coding sequences as in the mouse E-cadherin and chicken L-CAM genes. The transcription initiation site of the mouse P-cadherin gene has been located at about 68 nt from the ATG start codon, giving an estimation for the size of the first exon of the mouse P-cadherin gene of 116 bp. The sequence of the 5' upstream region of the P-cadherin gene presents structural similarities with the recently described 5' region of the mouse E-cadherin gene: absence of a TATA box, presence of a CAAT box at -65, two putative AP2-binding motifs, at -101 and +31, and a GC-rich region containing a potential SP1-binding element at -88. However, no sequence homologous to the palindromic sequence, E-pal, found on the E-cadherin promoter has been found in the 5' region of the P-cadherin gene. These results indicate that, in contrast to a previous report, the mouse E and P-cadherin genes exhibit a similar genomic organization both containing 15 introns and a similar size for the first two exons.
CSIC, Departamento de Bioquímica, Facultad de Medicina UAM, Madrid, Spain.
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Faraldo
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Cano
D000595:Amino Acid Sequence; D000818:Animals; D001482:Base Composition; D001483:Base Sequence; D015820:Cadherins / Q000235:genetics*; D004247:DNA; D007438:Introns; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid
D015820:Cadherins; D004247:DNA