A novel gene product associated with mu chains in immature B cells.
A previously unreported B cell specific gene, which we have named 8HS-20, was isolated from the cDNA library of a pre-B cell clone by subtraction and differential hybridization. This gene is selectively expressed as a 0.75 kb transcript in pre-B and bone marrow-derived B cell lines; a transcript of the same size is also found in bone marrow and, albeit at low levels, in spleen. The deduced amino acid sequence of the 8HS-20 cDNA displayed homology to a B cell specific gene, VpreB-1, and to members of the immunoglobulin supergene family including V lambda, V kappa, VH, TCRV alpha, V beta and CD8. Biochemical analysis using purified antiserum against 8HS-20 oligopeptides indicates that the gene encodes proteins with mol. wts of 13.5, 14, 15.5 and 16 kDa, which associate with mu chains in pre-B cell lines, and that these molecules are expressed concomitantly with VpreB-1 and lambda 5 gene products in the same cell lines.
Department of Immunology, NIH, Tokyo, Japan.
T
T
Shirasawa
K
K
Ohnishi
S
S
Hagiwara
K
K
Shigemoto
Y
Y
Takebe
K
K
Rajewsky
T
T
Takemori
D000595:Amino Acid Sequence; D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology / Q000378:metabolism*; D001483:Base Sequence; D001854:Bone Marrow Cells; D002460:Cell Line; D004247:DNA; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000378:metabolism*; D007148:Immunoglobulin mu-Chains / Q000235:genetics* / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D005810:Multigene Family; D054420:Pre-B Cell Receptors; D017386:Sequence Homology, Amino Acid; D013154:Spleen / Q000166:cytology
D007148:Immunoglobulin mu-Chains; D054420:Pre-B Cell Receptors; C515477:Vpreb3 protein, mouse; D004247:DNA