Differences in regulation of testis specifc lactate dehydrogenase in rat and mouse occur at multiple levels.
The testis specific form of lactate dehydrogenase (LDH-C4) is encoded by a single locus, Ldh-c, and is tightly regulated in a tissue specific manner. Here we show differences in expression of Ldh-c between rat and mouse, and describe the levels at which regulation of this gene differs in the two species. Our results demonstrate that the Ldh-c message level is nearly nine fold greater in mouse testis and remains high post-meiotically. In contrast, rat Ldh-c mRNA is highest in primary spermatocytes and reduced in spermatids. The results of nuclear run-on assays indicate that the transcription rate of Ldh-c is only moderately higher in mouse than rat, and cannot account for a significant portion of the observed differences. Similar decay rates for both rat and mouse Ldh-c mRNA in actinomycin-D clearance assays indicate comparable cytoplasmic stabilities for the two messages. From these results we infer that nuclear prostranscriptional events contribute to the differences in Ldh-c message levels.
Department of Biochemistry, Molecular Biology, and Cell Biology, Northwestern University, Evanston, Illinois 60208.
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Salehi-Ashtiani
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Goldberg
D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic; D007770:L-Lactate Dehydrogenase / Q000235:genetics / Q000378:metabolism*; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013045:Species Specificity; D013737:Testis / Q000201:enzymology*; D014158:Transcription, Genetic
D012333:RNA, Messenger; D004247:DNA; D007770:L-Lactate Dehydrogenase