Phosphatidylcholine-specific phospholipase C from Achromobacter xylosoxidans.
A new phosphatidylcholine-specific phospholipase C (EC 3.1.4.3.) has been isolated from the culture broth of Achromobacter xylosoxidans. Growth conditions were optimized for maximum production of the enzyme. A temperature-sensitive synthesis was established. Chromatography on CM Sephadex and polyacrylamide gel electrophoresis of the native enzyme revealed a number of isozymes. The water soluble substrate for phospholipase p-nitrophenylphosphorylcholine was not hydrolysed by the enzyme.
Department of Biochemistry and Microbiology, University of Plovdiv.
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Ivanov
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Kostadinova
D000421:Alcaligenes / Q000201:enzymology* / Q000254:growth & development; D003470:Culture Media; D006863:Hydrogen-Ion Concentration; D010713:Phosphatidylcholines / Q000378:metabolism*; D013379:Substrate Specificity; D010738:Type C Phospholipases / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism*
D003470:Culture Media; D010713:Phosphatidylcholines; D010738:Type C Phospholipases