Effects of restricted feeding on the absorption, metabolism, and accumulation of pentachlorobenzene in rats.
To investigate the effect of restricted feeding (RF) on the absorption, metabolism, and accumulation of pentachlorobenzene (PECB), rats were fed either ad libitum (AD) or a restricted diet (at 25 or 50% of AD) for 15 days. On the 7th day (day 0), PECB (15 mg/rat) was orally administered. Daily fecal weight and the total amount of PECB excreted into feces were decreased by RF, indicating an increase in PECB absorption due to its prolonged retention in the gastrointestinal tract. However, the amount of PECB excreted was only 4.8% of the dose given to the AD group, and 2.1 and 2.4% of that given to the 50 and 25% restricted diet groups, respectively. The concentrations of PECB and pentachlorophenol, and major metabolite, in blood of the restricted diet groups were higher on day 2 and day 4, while lower on day 6 and day 8 than those of the AD group. On day 8, PECB in liver, kidney, brain, and fat tissue were also lower in the restricted diet groups; the PECB levels in such tissue in the 25 and 50% restricted diet groups were 10-40 and 5-11% of that in the AD group, respectively. These results indicate that PECB metabolism was increased by RF. As PECB is lipophilic, it is most likely that the fat tissue mass, which was markedly decreased by RF, contributed to the enhancement of PECB metabolism. The mechanism seemed to be as follows: as fat tissue mass accumulating PECB decreased, the concentration of PECB in the liver increased, thus the amount of PECB metabolites formed was increased.
National Institute of Health and Nutrition, Tokyo, Japan.
K
K
Umegaki
S
S
Ikegami
T
T
Ichikawa
D000042:Absorption; D000273:Adipose Tissue / Q000378:metabolism; D000818:Animals; D001835:Body Weight; D001921:Brain / Q000378:metabolism; D002722:Chlorobenzenes / Q000097:blood / Q000493:pharmacokinetics*; D004064:Digestive System / Q000378:metabolism; D005243:Feces / Q000737:chemistry; D005508:Food Deprivation; D007668:Kidney / Q000378:metabolism; D007700:Kinetics; D008099:Liver / Q000378:metabolism; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D014018:Tissue Distribution
D002722:Chlorobenzenes; C003060:pentachlorobenzene