[Proteinases activated by gamma irradiation in the nuclei of rat peripheral blood leukocytes].
The nuclei of the peripheral blood leucocytes of rats displayed proteolytic activity (Ca-dependent with pH optimum 8.5 and active at alkaline pH), whose level increases under the influence of gamma-irradiation. These proteases were partially purified and identified. It was shown using gel chromatography, affinity chromatography with casein-sepharose and enzyme-electrophoresis that these proteolytic activities are related to one protein with a molecular mass of 24 kDa or a complex of proteins that do not cleave under the given experimental conditions. The both protease activities are predominantly blocked by inhibitors of cysteine proteases.
G N
GN
Surkenova
L V
LV
Malakhova
A I
AI
Gaziev
D000818:Animals; D002467:Cell Nucleus / Q000201:enzymology / Q000528:radiation effects*; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D010450:Endopeptidases / Q000097:blood / Q000302:isolation & purification / Q000528:radiation effects*; D004789:Enzyme Activation / Q000528:radiation effects; D005720:Gamma Rays; D006863:Hydrogen-Ion Concentration; D007962:Leukocytes / Q000201:enzymology / Q000528:radiation effects*; D008297:Male; D051381:Rats; D017208:Rats, Wistar
D010450:Endopeptidases
Aktiviruemye gamma-oblucheniem proteinazy iader leĭkotsitov perifericheskoĭ krovi krys.