Excystment in vitro of Cotylurus cornutus (Trematoda: Strigeidae) metacercariae.
An acid-medium pretreatment appeared to be essential for in vitro excystation of Cotylurus cornutus metacercarial cysts. Both acid-pepsin or acid-saline were effective but optimum excystation occurred after 15 min acid-pepsin pretreatment and subsequent 2 hr treatment at 41.5 C in an alkaline bile salt-trypsin medium. The rate of excystation was rapid, reaching 78.1% by 1 hr and increasing to 84.3% after 2 hr. Storage of metacercarial cysts up to 16 wk at 4 C in sterile Locke's 1:1 solution did not alter cyst viability.
Johns Hopkins University School of Hygiene and Public Health, Department of Immunology and Infectious Diseases, Baltimore, Maryland 21205.
T K
TK
Graczyk
C J
CJ
Shiff
D000818:Animals; D003470:Culture Media; D004372:Ducks / Q000469:parasitology; D006863:Hydrogen-Ion Concentration; D012908:Snails / Q000469:parasitology; D013696:Temperature; D014200:Trematoda / Q000502:physiology*
D003470:Culture Media