Correlation between phospholipase A2 activity and intra-Golgi protein transport reconstituted in a cell-free system.
A wide variety of phospholipase A2 inhibitors blocks intra-Golgi protein transport reconstituted in a cell-free system. Phospholipase A2 activity detectable under the protein transport assay conditions is actually inhibited by the inhibitors. There is a good correlation between the inhibition of protein transport and that of phospholipase A2 activity. Prolactin secretion from GH3 cells is also blocked by a membrane-permeable phospholipase A2 inhibitor, suggesting the physiological relevance to inhibition of protein transport in vitro by phospholipase A2 inhibitors.
Institute of Scientific and Industrial Research, Osaka University, Japan.
M
M
Tagaya
N
N
Henomatsu
T
T
Yoshimori
A
A
Yamamoto
Y
Y
Tashiro
T
T
Fukui
D000117:Acetylglucosamine / Q000378:metabolism; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D020126:Brefeldin A; D002474:Cell-Free System; D002478:Cells, Cultured; D003517:Cyclopentanes / Q000494:pharmacology; D005227:Fatty Acids / Q000494:pharmacology; D006056:Golgi Apparatus / Q000187:drug effects / Q000378:metabolism*; D009637:Masoprocol / Q000494:pharmacology; D008562:Membrane Glycoproteins; D010741:Phospholipases A / Q000032:analysis* / Q000037:antagonists & inhibitors; D054467:Phospholipases A2; D011388:Prolactin / Q000378:metabolism; D014759:Viral Envelope Proteins / Q000378:metabolism*; D062367:ortho-Aminobenzoates / Q000494:pharmacology
D003517:Cyclopentanes; D005227:Fatty Acids; C028391:G protein, vesicular stomatitis virus; D008562:Membrane Glycoproteins; D014759:Viral Envelope Proteins; D062367:ortho-Aminobenzoates; D020126:Brefeldin A; D009637:Masoprocol; D011388:Prolactin; D010741:Phospholipases A; D054467:Phospholipases A2; D000117:Acetylglucosamine