Breaking the adhesions of a cell with its substratum.
Investigation of the relative slowness with which trypsin detaches epithelial cells from their adhesions with a substratum suggests that the adherent surface of the cells is normally rather inaccessible to the enzyme. Trypsinisation of cells through a porous filter allowing access of the enzyme to the underside of the cells greatly accelerated detachment: a result consistent with this explanation.
Simpson
K
K
Department of Cell Biology, University of Glasgow, Scotland, UK.
McGrath
M
M
Curtis
A
A
D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects*; D002460:Cell Line / Q000187:drug effects; D004285:Dogs; D004848:Epithelium / Q000187:drug effects; D007365:Intercellular Junctions / Q000187:drug effects; D008567:Membranes, Artificial; D014357:Trypsin / Q000494:pharmacology*
D008567:Membranes, Artificial; D014357:Trypsin