Comparative immunohistolocalization of carbonic anhydrase isozymes I, II and III in the equine and bovine digestive tract.
Immunohistochemical localizations of carbonic anhydrase isozymes (CA-I, CA-II and CA-III) in equine and bovine digestive tracts were studied. In the horse, epithelial cells in both the oesophagus and non-glandular part of the stomach lacked all three isozymes. In contrast, surface epithelial and parietal cells in the glandular region of the stomach showed reactivity for CA-II. In the small intestine, absorptive columnar cells covering the villi in the duodenum were positive for CA-II. The epithelium of the jejunum and ileum lacked all three isozymes. In the large intestine, CA-II was detected in the columnar cells in the upper part of the crypt. In cattle, epithelial cells of the oesophagus showed reactions for CA-I and CA-III but not for CA-II. Although the absorptive epithelial cells of the small intestine lacked CA-I, CA-II and CA-III, those of the upper part of large intestine crypts were heavily stained for all three isozymes.
Department of Anatomy I, Azabu University School of Veterinary Medicine, Kanagawa, Japan.
K
K
Sasaki
S
S
Igarashi
T
T
Amasaki
H
H
Amasaki
T
T
Nishita
Y
Y
Kano
M
M
Asari
D000818:Animals; D000918:Antibody Specificity; D002256:Carbonic Anhydrases / Q000032:analysis*; D002417:Cattle / Q000378:metabolism*; D004064:Digestive System / Q000201:enzymology*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006736:Horses / Q000378:metabolism*; D007124:Immunoenzyme Techniques; D007527:Isoenzymes / Q000032:analysis*
D007527:Isoenzymes; D002256:Carbonic Anhydrases