Fluorescent estimation on the effect of Ca2+ antagonists on the oxidative metabolism in dissociated mammalian brain neurons.
Effect of organic Ca2+ antagonists on the oxidative metabolism or the formation of reactive oxygen species was estimated on dissociated mammalian neurons using a flow cytometer and 2',7'-dichlorofluorescin which is oxidized to be fluorescent by cellular oxidants. Of the organic Ca2+ antagonists used, flunarizine and nifedipine decreased the intensity of fluorescence at 1 microM or more while it was not the case for verapamil and diltiazem, suggesting one of the favourable actions of flunarizine and nifedipine on ischemic brain damage.
Oyama
Y
Y
Division of Pharmacological Science, Faculty of Integrated Arts and Sciences, University of Tokushima, Japan.
Hayashi
A
A
Ueha
T
T
Chikahisa
L
L
Furukawa
K
K
D000818:Animals; D001921:Brain / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D002121:Calcium Channel Blockers / Q000494:pharmacology*; D005434:Flow Cytometry; D005452:Fluoresceins; D066298:In Vitro Techniques; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D010084:Oxidation-Reduction; D051381:Rats; D017208:Rats, Wistar; D013050:Spectrometry, Fluorescence
D002121:Calcium Channel Blockers; D005452:Fluoresceins; C037631:2',7'-dichlorofluorescein