Analysis of the petunia nitrate reductase apoenzyme-encoding gene: a first step for sequence modification analysis.
In this paper, we describe the gene (nia) coding for the apoenzyme of the nitrate reductase (NR) of petunia. A full-size genomic clone was isolated from a genomic library, using the tobacco nia2 cDNA as a probe, and sequenced. The open reading frame is interrupted by three introns and encodes a protein of 909 amino acids which reveals between 92% and 68% identity to the NADH NR apoenzyme from other higher plants. Southern analyses indicated that the NR apoenzyme is encoded by a single-copy gene, although another region homologous to part of nia was also identified. The analysis of the steady-state level of nia mRNA showed that the petunia nia is regulated by the nitrogen source and is under the control of the circadian rhythm.
Laboratoire de Génétique Végétale, Université Paris-Sud, CNRS, Orsay, France.
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Salanoubat
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Bui Dang Ha
D000595:Amino Acid Sequence; D001051:Apoenzymes / Q000235:genetics*; D001483:Base Sequence; D002940:Circadian Rhythm; D003001:Cloning, Molecular; D004252:DNA Mutational Analysis; D015971:Gene Expression Regulation, Enzymologic; D017343:Genes, Plant; D008969:Molecular Sequence Data; D050901:Nitrate Reductase; D009565:Nitrate Reductases / Q000235:genetics*; D009584:Nitrogen / Q000378:metabolism; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000032:analysis; D012689:Sequence Homology, Nucleic Acid
D001051:Apoenzymes; D012333:RNA, Messenger; D009565:Nitrate Reductases; D050901:Nitrate Reductase; D009584:Nitrogen