The ERE-like sequence from the promoter region of the testis specific hsp70-related gene is not estrogen responsive.
The 5'flanking region of the hsp70-related gene which is specifically expressed in rat and mouse spermatocytes contains the GGTCAnnnCGACC sequence closely resembling the palindromic consensus ERE (estrogen responsive element, GGTCAnnnTGACC). We used a transient expression assay to study whether this ERE-like sequence can transfer estrogen responsiveness either to heterologous or homologous promoters linked to a reporter CAT gene. We found that the GGTCAnnnCGACC sequence either as an isolated element or in its natural sequence context does not respond to hormone stimulation in MCF-7 and Fe33 cells. This observation points to the importance of the T-nucleotide in a right half-palindrome of ERE for ligand-dependent activation of transcription.
Department of Tumor Biology, Institute of Oncology, Gliwice, Poland.
Z
Z
Krawczyk
W
W
Schmid
P
P
Härkönen
P
P
Wolniczek
D000818:Animals; D001483:Base Sequence; D016384:Consensus Sequence; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D006360:Heat-Shock Proteins / Q000235:genetics*; D006801:Humans; D008024:Ligands; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000187:drug effects*; D051381:Rats; D013737:Testis; D014158:Transcription, Genetic; D014162:Transfection; D014407:Tumor Cells, Cultured / Q000187:drug effects
D006360:Heat-Shock Proteins; D008024:Ligands; D004958:Estradiol