Ultrasonication of lamb skeletal muscle fibres enhances postmortem proteolysis.
The effect of ultrasound upon the postmortem proteolytic activity of muscle fibres was investigated. As a preliminary result it was demonstrated that ultrasonication released lysosomal enzymes from liver cells while cell membranes suffered little damage. Proteolysis brought about by endogenous proteinases after 2 days of fibre storage at 4 degrees C was assessed by means of sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). Ultrasonic treatment of fibres enhanced proteolytic activity, as shown by the increased intensity of 30-kDa region degradation bands. A distinct change featuring sonicated fibres was the degradation of an 87-kDa protein and the appearance of an 83-kDa peptide. Cell damage was not very extensive, although it depended upon ultrasonication and fibre conditions.
Roncalés
P
P
Departamento de Producción Animal y Ciencia de los Alimentos, Facultad de Veterinaria, Universidad de Zaragoza, Spain.
Ceña
P
P
Beltrán
J A
JA
Jaime
I
I
D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D010450:Endopeptidases / Q000378:metabolism*; D005519:Food Preservation; D008099:Liver / Q000000981:diagnostic imaging / Q000201:enzymology; D008247:Lysosomes / Q000000981:diagnostic imaging / Q000201:enzymology; D008460:Meat / Q000592:standards*; D009124:Muscle Proteins / Q000378:metabolism; D009132:Muscles / Q000000981:diagnostic imaging* / Q000201:enzymology; D011180:Postmortem Changes; D012756:Sheep; D013010:Sonication; D014463:Ultrasonography
D009124:Muscle Proteins; D000135:Acid Phosphatase; D010450:Endopeptidases