Functional and morphological effects of defibrotide on renal ischemia.
Defibrotide (Df) has been reported to protect various organs from ischemic damage. The aim of this study was to evaluate the effect of Df on renal function and morphology after warm kidney ischemia. Divided into two groups, 14 pigs underwent bilateral renal clamping for 90 min. One group (7 pigs) was treated with Df (32 mg/kg per h) for 6 h, whereas the control group received 5000 IU of heparin. Serum levels of blood urea nitrogen (BUN) and creatinine measured for 6 days were significantly higher in the control group (peak 26.8 +/- 16.7 vs 11.0 +/- 2.9 mmol/l and 501.2 +/- 351.4 vs 230.2 +/- 68.0 mumol/l P < 0.05). Renal biopsy evidenced a lesser extent of tubular and endothelial damage in Df-treated animals. In conclusion, Df provided relevant protection against renal ischemic injury.
Istituto di Semeiotica Chirurgica, University of Padua, Padova, Italy.
M G
MG
Comandella
P
P
Rigotti
M
M
Valente
G
G
Pittoni
N
N
Baldan
E
E
Ganz
P
P
Amodio
E
E
Ancona
D000818:Animals; D005343:Fibrinolytic Agents / Q000494:pharmacology*; D007511:Ischemia / Q000473:pathology* / Q000503:physiopathology*; D007668:Kidney / Q000098:blood supply* / Q000187:drug effects / Q000648:ultrastructure*; D007677:Kidney Function Tests; D011089:Polydeoxyribonucleotides / Q000494:pharmacology*; D012079:Renal Circulation / Q000187:drug effects; D013552:Swine
D005343:Fibrinolytic Agents; D011089:Polydeoxyribonucleotides; C036901:defibrotide