Interaction between tumor necrosis factor-alpha and HSP 70 in human leukemia cells.
We confirm here by immunoblotting that a cell line resistant to clonal inhibition from tumor necrosis factor (K562) expresses high levels of HSP 70, whereas two sensitive cell lines (HL60 blast and EM3) express low levels. When sensitive cell lines are exposed to tumor necrosis factor, HSP 70 is not generally induced; when tumor necrosis factor is removed, however, HSP 70 is transiently induced. In five bone marrow samples from patients, generally low levels of HSP 70 were detected.
GSF Institute für Klinische Hämatologie, München, F.R.G.
E
E
Müller
R
R
Munker
R
R
Issels
W
W
Wilmanns
D015153:Blotting, Western; D002460:Cell Line; D006360:Heat-Shock Proteins / Q000302:isolation & purification / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D011994:Recombinant Proteins / Q000494:pharmacology; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
D006360:Heat-Shock Proteins; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha