Determination of fluoxetine and norfluoxetine in human plasma by capillary gas chromatography with electron-capture detection.
A capillary gas chromatographic method with 63Ni electron-capture detection is reported for the determination of fluoxetine (Prozac) and its metabolite norfluoxetine in human plasma. A liquid-liquid extraction is used, followed by derivatization with heptafluorobutyric anhydride to increase the sensitivity of detection. A 30 m x 0.25 mm I.D. DB-17 capillary column resolves the compounds from endogenous matrix interferences. The limit of quantitation by this method is 5 ng/ml for each compound. Stability studies show that fluoxetine and norfluoxetine are stable in human plasma for up to 96 h at room temperature and up to one year at -20 degrees C.
Lantz
R J
RJ
Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, IN 46285.
Farid
K Z
KZ
Koons
J
J
Tenbarge
J B
JB
Bopp
R J
RJ
D002138:Calibration; D002849:Chromatography, Gas / Q000379:methods*; D004355:Drug Stability; D004583:Electrons; D005466:Fluorocarbons; D005473:Fluoxetine / Q000031:analogs & derivatives* / Q000097:blood*; D006801:Humans; D007202:Indicators and Reagents; D015203:Reproducibility of Results; D014050:Toluene
D005466:Fluorocarbons; D007202:Indicators and Reagents; D005473:Fluoxetine; C007924:heptafluorobutyric anhydride; D014050:Toluene; C036139:norfluoxetine