Albumin grafting on to polypropylene by thermal activation.
4-azido-2-nitrophenyl albumin (ANP-albumin) was prepared by reacting 4-fluoro-3-nitrophenyl azide with albumin. The thermal decomposition kinetics of phenyl azide of ANP-albumin was studied at various temperatures by Fourier-Transform Infrared (FTIR) spectroscopy. The decomposition rate of the phenyl azide increased with temperature. The activation energy for the first-order decomposition of the phenyl azide was 128.0 kJ/mol. Albumin was grafted on to polypropylene (PP) films by thermolysis of the azido groups of ANP-albumin with no premodification of the PP surface. The albumin-grafted surface was characterized by electron spectroscopy for chemical analysis (ESCA) and by quantitative determination of platelet adhesion and activation. The bulk concentration of ANP-albumin used for adsorption varied from 0.001 to 30 mg ml-1, and the albumin-adsorbed PP films were incubated at 100 degrees C for up to 7 h. The carbon and nitrogen peaks resulting from the grafted albumin were used to compare the surface albumin concentrations as a function of the concentration of ANP-albumin in the adsorption solution. When the PP film was adsorbed with ANP-albumin at the concentration of 5 mg ml-1 or higher and incubated at 100 degrees C for longer than 5 h, the surface became resistant to platelet adhesion. The ANP-albumin can be grafted on to chemically inert surfaces such as PP surface through simple thermolysis of azido groups to prevent platelet adhesion and activation.
School of Pharmacy, Purdue University, West Lafayette, IN 47907.
Y C
YC
Tseng
W M
WM
Mullins
K
K
Park
D000327:Adsorption; D001386:Azides / Q000138:chemical synthesis / Q000737:chemistry*; D002448:Cell Adhesion; D005583:Fourier Analysis; D006358:Hot Temperature; D006801:Humans; D007700:Kinetics; D008855:Microscopy, Electron, Scanning; D015539:Platelet Activation; D010973:Platelet Adhesiveness; D011126:Polypropylenes / Q000737:chemistry*; D012709:Serum Albumin / Q000138:chemical synthesis / Q000737:chemistry*
C081659:4-azido-2-nitrophenylalbumin; D001386:Azides; D011126:Polypropylenes; D012709:Serum Albumin; C007753:4-fluoro-3-nitrophenyl azide