Rat retinal ganglion cells express Ca(2+)-permeable non-NMDA glutamate receptors during the period of histogenetic cell death.
Local application of glutamate agonists to retinal ganglion cells (RGNs) was performed in retinae isolated from pigmented rats aged between 3 and 8 days postnatally. A vast majority of RGNs displayed current responses to glutamate (Glu), N-methyl-D-aspartate (NMDA), quisqualate (QA), alpha-amino-2,3-dihydro-5-methyl-3-oxo-4-isoazolepropanoic acid (AMPA), kainate (KA) and domoate (DA). In Na(+)-free extracellular solution with elevated Ca2+, non-NMDA agonists elicited large (up to 200 pA) inward currents that were completely blocked by 6,7-dinitroquinoxaline-2,3-dione (DNQX) and Cd2+. MK-801 also induced a partial block of cationic currents in Na(+)-free saline. In standard salt solutions, current-voltage relationships of Glu-R-mediated currents were often inwardly rectifying in the presence of D-aminophosphonovalerat (D-APV), as is typical of Ca(2+)-permeable non-NMDA receptors. The presence of inward rectification in the current voltage relationship was always associated with a high value of the cationic permeability ratio PCa2+/PCs+ (> 0.8). However, in about half of the investigated RGNs no inward rectification was observed under standard recording conditions. Our results lead to the suggestion that expression of Ca(2+)-permeable Glu receptor subunits may contribute to regulation of cell numbers in the postnatal retina.
Rörig
B
B
Max-Planck-Institute for Psychiatry, Developmental Neurobiology/BMFT, Martinsried, FRG.
Grantyn
R
R
D000596:Amino Acids / Q000494:pharmacology; D000818:Animals; D002104:Cadmium / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D016923:Cell Death; D016291:Dizocilpine Maleate / Q000494:pharmacology; D066298:In Vitro Techniques; D010539:Permeability; D011810:Quinoxalines / Q000494:pharmacology; D051381:Rats; D017470:Receptors, Glutamate / Q000378:metabolism*; D012165:Retinal Ganglion Cells / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology
D000596:Amino Acids; D011810:Quinoxalines; D017470:Receptors, Glutamate; D002104:Cadmium; C056723:FG 9041; D016291:Dizocilpine Maleate; D002118:Calcium