Determination of argininosuccinate lyase in human serum by ion-pair reversed phase liquid chromatography.
A new assay for argininosuccinate lyase based on the separation of the enzymatic reaction mixture components by an ion-pair reversed phase mechanism is reported. The determination of enzyme activity is performed after direct injection by UV detection of the fumarate formed. The chromatographic analysis time is 8 min and a detection limit of 0.4 U/L is achieved. The HPLC method is highly accurate, sensitive and precise. The simple procedure makes this method suitable for the routine determination of ASAL activity in human serum samples.
Canepari
S
S
Department of Chemistry, University of Rome La Sapienza, Italy.
Carunchio
V
V
Girelli
A M
AM
Messina
A
A
D001120:Arginine / Q000378:metabolism; D001123:Argininosuccinate Lyase / Q000097:blood*; D001125:Argininosuccinic Acid / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000706:statistics & numerical data; D005650:Fumarates / Q000378:metabolism; D006801:Humans; D012680:Sensitivity and Specificity
D005650:Fumarates; D001125:Argininosuccinic Acid; C032005:fumaric acid; D001120:Arginine; D001123:Argininosuccinate Lyase