Cloning of a DNA probe and its application in the detection of Plasmodium falciparum. A preliminary report.
The clones containing parasite DNA fragments were screened from a genomic DNA library of Plasmodium falciparum FCC1/HN isolate. A DNA probe derived from clone pBF4 consisting of 3 kilobase pairs hybridizes specifically with P. falciparum DNA but not with human DNA, P. cynomolgi DNA or P. berghei DNA. The nick translated radiolabelled probe can detect 10 pg purified P. falciparum DNA and a 0.001% parasitemia after 24 hours of film exposure. The probe reacts with all microscopically diagnosed P. falciparum samples and 3 of 41 P. vivax samples as well but not with any of 10 human DNA samples.
Shandong Institute of Parasitic Diseases, Jining.
K Y
KY
Liu
B C
BC
Huang
H H
HH
Zhang
Y B
YB
Liu
R Q
RQ
Kong
Y L
YL
Cheng
D000328:Adult; D000368:Aged; D000818:Animals; D015342:DNA Probes; D016054:DNA, Protozoan / Q000032:analysis*; D015698:Genomic Library; D006801:Humans; D008288:Malaria / Q000469:parasitology; D008875:Middle Aged; D009693:Nucleic Acid Hybridization; D010963:Plasmodium falciparum / Q000302:isolation & purification*; D013045:Species Specificity
D015342:DNA Probes; D016054:DNA, Protozoan