Transferred T lymphocytes are compromised when the donor is pretreated with beta-endorphin.
A single intracerebroventricular (i.c.v.) injection of 14 pmol of beta-endorphin into 6-7-week-old BALB/c (+/+) donor mice, 24 h prior to isolation of their T lymphocytes for use for reconstitution of athymic BALB/c (nu/nu) nude mice, altered the immuno-protective effect of adoptive transfer against an intracerebral (i.c.) infection with a temperature-sensitive mutant of vesicular stomatitis virus (tsG31 KS5 VSV). Simultaneous injection of beta-endorphin and naloxone into donor animals negated the opiate effects on splenic lymphocytes. T lymphocytes, isolated from beta-endorphin-treated donors, and then depleted with anti-asialo GM1 antiserum and complement failed to demonstrate the detrimental effects of beta-endorphin.
Center for Basic Cancer Research and BioServe Space Technologies, Kansas State University, Manhattan.
W J
WJ
Coons
R W
RW
Vorhies
T C
TC
Johnson
D000818:Animals; D005677:G(M1) Ganglioside / Q000276:immunology; D007276:Injections, Intraventricular; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009270:Naloxone / Q000494:pharmacology; D013601:T-Lymphocytes / Q000276:immunology / Q000637:transplantation*; D001615:beta-Endorphin / Q000008:administration & dosage / Q000494:pharmacology*
D009270:Naloxone; D005677:G(M1) Ganglioside; D001615:beta-Endorphin; C018835:asialo GM1 ganglioside