Levels of androgens and danazol metabolites in serum during danazol therapy.
Thirteen women who had endometriosis were treated with danazol (300 to 400 mg/d). Levels of androgens and danazol metabolites in serum and the influences of danazol metabolites on the assays for serum androgen were investigated during danazol therapy. Serum DHEAS significantly increased (P < 0.05), but serum DHEA slightly decreased. Serum T levels, measured by direct assay, were markedly elevated. However, measured after HPLC separation, the T levels in serum were significantly decreased (P < 0.05). There were considerable cross-reactions between danazol metabolites and androgens (T, DHEA, and A) in RIA. Purification of androgens using column chromatography was necessary to measure serum androgens precisely.
Department of Obstetrics and Gynecology, Kanazawa University School of Medicine, Japan.
K
K
Murakami
T
T
Nakagawa
G
G
Yamashiro
K
K
Araki
K
K
Akasofu
D000328:Adult; D000728:Androgens / Q000097:blood*; D003613:Danazol / Q000097:blood* / Q000627:therapeutic use; D003687:Dehydroepiandrosterone / Q000097:blood; D004715:Endometriosis / Q000097:blood* / Q000188:drug therapy; D005260:Female; D006801:Humans; D008875:Middle Aged; D013739:Testosterone / Q000097:blood
D000728:Androgens; D013739:Testosterone; D003687:Dehydroepiandrosterone; D003613:Danazol