[Use of buserelin in a fertilization in vitro-embryo transfer program and determination of steroid concentrations of preovulatory follicular fluid].
Eleven infertility patients, stimulated by Buserelin/hMG/hCG protocol (BHh group) for superovulation, were compared with thirteen patients using CC/hMG/hCG protocol (CHh group) in an IVF-ET program. The rates of oocyte retrieval, fertilization and cleavage in BHh group were significantly, higher than those in CHh group. Furthermore, the incidence of premature LH surge in BHh group was 0% and 23.1% in CHh group. Eventually there were four pregnancies in BHh group and only one in CHh group. The mean concentrations of estradiol (E2), progesterone (P) and P/E2 ratio in follicular fluid (FF) in BHh group were significantly lower than that in CHh group. The values of FF P/E2 ratio in the range of 10-50 were positively correlated with oocyte fertilization rates. These data suggested that the addition of Buserelin in the superovulation protocol improved the outcome of IVF-ET treatment.
First Affiliated Hospital, Sun Yat-Sen University of Medical Sciences, Guangzhou.
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Zhuang
D000328:Adult; D002064:Buserelin / Q000494:pharmacology*; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D004624:Embryo Transfer; D004958:Estradiol / Q000378:metabolism; D005260:Female; D005307:Fertilization in Vitro; D015571:Follicular Fluid / Q000378:metabolism*; D006801:Humans; D007247:Infertility, Female / Q000628:therapy; D010062:Ovulation Induction / Q000379:methods*; D011374:Progesterone / Q000378:metabolism; D013480:Superovulation / Q000187:drug effects
D006063:Chorionic Gonadotropin; D011374:Progesterone; D004958:Estradiol; D002064:Buserelin