p53 expression and K-ras mutation in colorectal adenomas.
The frequency of p53 overexpression and K-ras codon 12 mutation was investigated in a series of colorectal adenomas. p53 was detected by immunohistochemistry in only 5% of tumours, whereas K-ras mutation was found in eight of 30 adenomas examined. In vitro, mutant p53 and ras genes cooperate to transform primary rat cells into a tumourigenic cell line. The presence of both p53 overexpression and K-ras mutation in a benign tubulovillous polyp in the present series suggests that in vivo this combination of events is insufficient to cause malignant transformation of a large bowel adenoma.
Academic Unit of Pathology, University of Leeds.
N
N
Scott
S M
SM
Bell
P
P
Sagar
G E
GE
Blair
M F
MF
Dixon
P
P
Quirke
D000236:Adenoma / Q000737:chemistry / Q000235:genetics*; D015179:Colorectal Neoplasms / Q000737:chemistry / Q000235:genetics*; D016158:Genes, p53 / Q000235:genetics*; D011905:Genes, ras / Q000235:genetics*; D006801:Humans; D007150:Immunohistochemistry; D007417:Intestinal Polyps / Q000737:chemistry / Q000235:genetics; D009154:Mutation / Q000235:genetics*; D016159:Tumor Suppressor Protein p53 / Q000032:analysis*
D016159:Tumor Suppressor Protein p53