Quantitative analysis of cefradine by liquid chromatography on poly(styrene-divinylbenzene).
A method is described for isocratic analysis of cefradine by liquid chromatography on a poly(styrene-divinylbenzene) column (PLRP-S, 250 x 4.6 mm i.d.) at 50 degrees C. Cefradine is separated from its related substances using a mobile phase of acetonitrile-0.02 M sodium 1-octanesulphonate-0.2 M phosphoric acid-water (14.5:10:5:up to 100, v/v/v/v). The flow rate was 1.0 ml min-1 and UV-detection was performed at 254 nm. The method was employed for the quantitative analysis of reference substances, bulk samples and pharmaceutical dosage forms.
Katholieke Universiteit Leuven, Laboratorium voor Farmaceutische Chemie, Belgium.
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C
Hendrix
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Pijcke
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LM
Yun
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E
Roets
J
J
Hoogmartens
D000097:Acetonitriles; D002515:Cephradine / Q000032:analysis*; D002853:Chromatography, Liquid; D000432:Methanol; D011137:Polystyrenes / Q000737:chemistry*; D012015:Reference Standards; D013056:Spectrophotometry, Ultraviolet
D000097:Acetonitriles; D011137:Polystyrenes; C003771:Amberlite XAD-2 resin; D002515:Cephradine; D000432:Methanol; C032159:acetonitrile