Cloning and sequencing of goldfish activin subunit genes: strong structural conservation during vertebrate evolution.
Inhibin and activin are structurally related dimeric peptides sharing two distinct but related subunits, beta A and beta B. By using polymerase chain reaction (PCR), we have cloned and sequenced activin beta A and beta B genes encoding the mature region of the peptides from the goldfish genomic DNA. A single form of beta A and two forms of beta B subunits were found. There is high identity with mammalian counterparts; the goldfish beta A subunit has 78% protein sequence identity with human beta A subunit, and the two beta B subunits have more than 94% sequence identity with human beta B subunit. This is the first demonstration of the activin family of peptides in the fishes.
Department of Zoology, University of Alberta, Edmonton, Canada.
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Gallin
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Strobeck
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Peter
D028341:Activins; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005075:Biological Evolution; D015139:Blotting, Southern; D003001:Cloning, Molecular; D017124:Conserved Sequence; D004247:DNA; D006054:Goldfish / Q000235:genetics*; D006133:Growth Substances / Q000235:genetics; D015186:Hominidae / Q000235:genetics; D006801:Humans; D007265:Inhibins / Q000235:genetics*; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D016133:Polymerase Chain Reaction; D051381:Rats / Q000235:genetics; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D013552:Swine / Q000235:genetics; D014714:Vertebrates / Q000235:genetics*; D014981:Xenopus / Q000235:genetics
D006133:Growth Substances; D046911:Macromolecular Substances; D009838:Oligodeoxyribonucleotides; D028341:Activins; D007265:Inhibins; D004247:DNA