Separation of high-density lipoproteins into apolipoprotein E-poor and apolipoprotein E-rich subfractions by fast protein liquid chromatography using a heparin affinity column.
The aim of this paper is to describe a new methodology for the separation of human high-density lipoproteins (HDL) into apolipoprotein (apo) E-poor and apo E-rich subfractions by fast protein liquid chromatography (FPLC) using a heparin affinity column. Recoveries for apolipoproteins AI, AII, CI, CII, CIII, and E were 68.9, 74.7, 71.9, 73.5, 40.0, and 55.8%, respectively. We provide suggestive evidence that apo E-rich HDL is produced from apo E-poor HDL by the displacement of apo AI by apo E. Apo E-poor HDL was the predominant fraction. The molar ratio of apo E to apo AI in apo E-poor HDL was 0.02 and 0.01 for the subjects studied while in apo E-rich HDL it was 1.86 and 1.25. The molar ratios of the C apolipoproteins to apo AI are markedly different between the subfractions.
O'Brien
T
T
Atherosclerosis Research Unit, Mayo Clinic and Foundation, Rochester, MN 55905.
Buithieu
J
J
Nguyen
T T
TT
Klein
L
L
Bren
N
N
Wentworth
M
M
Hallaway
B J
BJ
Kottke
B A
BA
D001053:Apolipoproteins / Q000302:isolation & purification; D001057:Apolipoproteins E / Q000302:isolation & purification*; D002853:Chromatography, Liquid; D006493:Heparin; D006801:Humans; D008074:Lipoproteins / Q000302:isolation & purification*; D008075:Lipoproteins, HDL / Q000302:isolation & purification
D001053:Apolipoproteins; D001057:Apolipoproteins E; D008074:Lipoproteins; D008075:Lipoproteins, HDL; D006493:Heparin