Cloning of the mouse interleukin 2 receptor gamma chain: demonstration of functional differences between the mouse and human receptors.
We isolated a cDNA clone for the gamma chain of the mouse interleukin 2 receptor. Introduction of the mouse gamma chain cDNA clone into a mouse fibroblast cell line, L929, expressing the mouse alpha beta heterodimer IL-2 receptor converted pseudo-high affinity of the IL-2 receptor into functional high, resulting in internalization of IL-2 and induction of the c-myc, c-fos and c-jun genes. The mouse beta gamma heterodimer, however, failed to bind IL-2 unlike the human beta gamma heterodimer intermediate-affinity receptor. These results indicate that the mouse functional IL-2 receptor is a complex comprising three distinct subunits, alpha, beta and gamma chains, but the beta gamma heterodimer is not functional and different from the human heterodimer.
Kumaki
S
S
Department of Microbiology, Tohoku University School of Medicine, Sendai, Japan.
Kondo
M
M
Takeshita
T
T
Asao
H
H
Nakamura
M
M
Sugamura
K
K
D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D003001:Cloning, Molecular; D004247:DNA; D006801:Humans; D007376:Interleukin-2 / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D015375:Receptors, Interleukin-2 / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
D007376:Interleukin-2; D015375:Receptors, Interleukin-2; D004247:DNA