Variability of 11q23 rearrangements in hematopoietic cell lines identified with fluorescence in situ hybridization.
We mapped and ordered 17 cosmid, phage, and plasmid clones to chromosome 11, bands q22-q24, using fluorescence in situ hybridization (FISH). We then analyzed four hematopoietic cell lines with 11q23 rearrangements, Karpas 45, SUP-T13, RC-K8, and Karpas 422, using these probes. The studies showed that the translocation breakpoints of the Karpas 45 and SUP-T13 cell lines, which were derived from T-cell malignancies, were located in the same breakpoint cluster region of the MLL gene as the RS4; 11 cell line and patients with the t(9;11), t(11;19), and t(6;11) described previously. We confirmed that the translocation breakpoint of the RC-K8 cell line was located telomeric to the MLL gene, and found that the derivative 11 chromosome of the Karpas 422 cell line, which had been thought to contain a t(4;11) (q21;q23), was in fact formed through a deletion and an inverted tandem repeat of part of 11q.
Department of Medicine, University of Chicago, IL 60637.
H
H
Kobayashi
R
R
Espinosa
M J
MJ
Thirman
E M
EM
Davis
M O
MO
Diaz
M M
MM
Le Beau
J D
JD
Rowley
D002460:Cell Line; D002874:Chromosome Mapping; D002880:Chromosomes, Human, Pair 11; D015342:DNA Probes; D015321:Gene Rearrangement; D006412:Hematopoietic Stem Cells / Q000648:ultrastructure*; D006801:Humans; D017403:In Situ Hybridization; D014178:Translocation, Genetic
D015342:DNA Probes