Phi X174 E complements lambda S and R dysfunction for host cell lysis.
Hybrid lambda phages which have the E lysis gene of the bacteriophage phi X174 in cis to defective nonsense and deletion alleles of the normal lambda lysis genes S and R have been constructed and shown to be fully competent for plaque-forming ability, which demonstrates that the single-gene, lysozyme-independent lysis system of phi X174 and related phages can serve the lytic function for large complex phages. These hybrid phages are unable to form plaques on a slyD host. Moreover, plaque morphology indicates that in E-mediated lysis the soluble lambda R endolysin can participate in lysis, indicating that the protein E-mediated lesions are not completely sealed off from the periplasm.
Department of Biochemistry and Biophysics, Texas A&M University, College Station 77843-2128.
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D010582:Bacteriophage lambda / Q000235:genetics* / Q000254:growth & development; D010584:Bacteriophage phi X 174 / Q000235:genetics* / Q000254:growth & development; D010450:Endopeptidases / Q000378:metabolism; D005814:Genes, Viral; D005816:Genetic Complementation Test; D016695:Glycosyltransferases / Q000378:metabolism*; D014764:Viral Proteins / Q000378:metabolism*; D015678:Viral Structural Proteins / Q000235:genetics; D014779:Virus Replication
C029555:E protein, bacteriophage X174; D014764:Viral Proteins; D015678:Viral Structural Proteins; D016695:Glycosyltransferases; D010450:Endopeptidases; C001143:endolysin