[Immunobiological characteristics of Brucella outer membrane proteins, genetic determinants of which are expressed in Escherichia coli cells].
The influence of the brucella OMP preparations obtained by the genetic engineering technique (18, 38 and 18 + 38 kD) on the formation of the specific protection and progress of infectious process in brucellosis in the in vivo experiments has been studied. The OMPs synthesized in Escherichia coli cells GSE579 and having mol mass 18 and 38 kD are common antigens for a number of brucella species. The 18 kD OMP was found to protect 66.7% of experimental animals against brucellosis, while the protection by the commercial live vaccine was 78.0%. The 38 kD OMP did not possess the protective activity with the index of infectivity in the experimental group of animals being higher than the one in the control group (77.9% and 53.4% respectively). The indexes of colony forming units in the mice spleen were also higher in the experimental group of animals. The obtained results suggest that the 38 kD OMP may be a factor of brucella virulence.
D F
DF
Seliutina
V E
VE
Malikov
V A
VA
Chibisova
V N
VN
Gorelov
A G
AG
Skavronskaia
D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000276:immunology*; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000276:immunology; D002003:Brucella abortus / Q000276:immunology* / Q000472:pathogenicity; D017347:Brucella melitensis / Q000276:immunology* / Q000472:pathogenicity; D002006:Brucellosis / Q000276:immunology / Q000382:microbiology; D004926:Escherichia coli; D015870:Gene Expression; D006168:Guinea Pigs; D051379:Mice; D008807:Mice, Inbred BALB C; D014774:Virulence / Q000235:genetics
D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines
Immunobiologicheskaia kharakteristika belkov naruzhnoĭ membrany brutsell, geneticheskie determinanty kotorykh ékspressiruiutsia v kletkakh Escherichia coli.