Leptospira genomes are modified at 5'-GTAC.
Genomic DNAs of 14 strains from seven species of the spirochete Leptospira were resistant to cleavage by the restriction endonuclease RsaI (5'-GTAC). A modified base comigrating with m4C was detected by chromatography. Genomic DNAs from other spirochetes, Borrelia group VS461, and Serpulina strains were not resistant to RsaI digestion. Modification at 5'-GTAm4C may occur in most or all strains of all species of Leptospira but not in all genera of spirochetes. Genus-wide DNA modification has rarely been observed in bacteria.
Ralph
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California Institute of Biological Research, La Jolla 92037.
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Van Etten
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McClelland
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D044503:5-Methylcytosine; D001483:Base Sequence; D003596:Cytosine / Q000031:analogs & derivatives / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics*; D007919:Leptospira / Q000235:genetics*; D008745:Methylation; D015183:Restriction Mapping
D004269:DNA, Bacterial; D044503:5-Methylcytosine; D003596:Cytosine