Measles virus haemagglutinin induces down-regulation of gp57/67, a molecule involved in virus binding.
A surface glycoprotein (gp57/67) was previously shown to be involved in measles virus (MV) binding and characterized in our laboratory. Here, we described down-regulation of cell surface gp57/67 after infection with MV. This effect is specific for MV since cells infected with canine distemper virus, closely related to MV, did not down-regulate gp57/67. The decrease in cell surface gp57/67 correlated with expression of MV glycoproteins and more particularly with the expression of MV haemagglutinin (MV-H). Indeed, expression of MV-H after infection with a vaccinia virus recombinant coding for MV-H was necessary and sufficient to induce down-regulation of gp57/67. Kinetics of cell surface expression of MV-H and gp57/67 showed that the degree of down-regulation was correlated with the amount of MV-H expressed by infected cells. Experiments using antibody-prelabelled gp57/67 and indirect immunofluorescence microscopy allowed us to follow the fate of gp57/67 and showed that down-regulation was occurring by rapid internalization of gp57/67 from the cell surface. These results provide additional evidence that the gp57/67 molecule is closely associated with the pathway of MV infection and also reveal a phenomenon which may be related to viral pathogenesis and persistence.
Immunobiologie Moléculaire CNRS-ENS UMR 49, Lyon, France.
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Naniche
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Wild
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Rabourdin-Combe
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Gerlier
D001692:Biological Transport; D002460:Cell Line; D015536:Down-Regulation; D004847:Epithelial Cells; D004848:Epithelium / Q000382:microbiology; D006389:Hemagglutinins, Viral / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008459:Measles virus / Q000254:growth & development*; D008562:Membrane Glycoproteins / Q000096:biosynthesis*; D011991:Receptors, Virus / Q000096:biosynthesis*; D011994:Recombinant Proteins / Q000378:metabolism; D013601:T-Lymphocytes / Q000166:cytology / Q000382:microbiology; D014616:Vaccinia virus / Q000235:genetics
D006389:Hemagglutinins, Viral; D008562:Membrane Glycoproteins; D011991:Receptors, Virus; D011994:Recombinant Proteins; C081691:gp57-67 binding protein