[Comparative study of 2 culture methods by seeding, in hemoculture bottles, the dialysis fluid from patients in continuous ambulatory peritoneal dialysis].
BACKGROUND
Peritonitis remains a major complication of continuous ambulatory peritoneal dialysis (CAPD). The accurate diagnosis of peritonitis is a requirement for a successful CAPD program. A prospective study was performed to evaluate two culture methods.


METHODS
1. Culture of 10 ml of uncentrifuged peritoneal fluid and 2. Culture of the sediment of 50 ml centrifuged and resuspended in 20 ml of distilled water, into aerobe/anaerobe hemoculture bottles (Hemoline, BioMérieux). We processed 162 PF from 138 CAPD peritonitis episodes, 33 of whom were in antimicrobial drug therapy.


RESULTS
The sensibility/specificity of both methods (87.0/70.8 method 1 and 81.9/79.2) were similar. Both methods were more sensitive (p = 0.001) when the patient was no in antimicrobial drug therapy (60.6/88.6 method 1 and 69.7/92.4 method 2). We isolated 132 microorganisms, 64.4% Gram positive, 25.0% Gram negative, 4.6% anaerobes and 6.1% levures.


CONCLUSIONS
Both methods were similar and the more important factor to increase the yield of culture is to remove the antibiotic presents in centrifuged fluid.
Seccione de Microbiología, Hospital de Galdakao, Vizcaya.
M J
MJ
Echeverría
R
R
Ayarza
M J
MJ
López de Goicoechea
M
M
Gómez-Corral
A
A
Barbier
A B
AB
Ibarretxebea
Y
Y
Vecino
J
J
Montenegro
D001424:Bacterial Infections / Q000175:diagnosis* / Q000382:microbiology; D001431:Bacteriological Techniques; D002177:Candidiasis / Q000175:diagnosis* / Q000382:microbiology; D006801:Humans; D009172:Mycology / Q000379:methods; D010531:Peritoneal Dialysis, Continuous Ambulatory / Q000009:adverse effects*; D010538:Peritonitis / Q000382:microbiology*; D012680:Sensitivity and Specificity
Estudio comparativo de dos métodos de cultivo, por siembra en frascos de hemocultivo, de líquidos de diálisis en pacientes en diálisis peritoneal continua ambulatoria.