Isolation of cDNA encoding a binding protein specific to 5'-phosphorylated single-stranded DNA with G-rich sequences.
We have isolated the cDNA encoding a binding protein to the sequence motif of the immunoglobulin S mu region by the southwestern method. The binding protein designated S mu bp-2 specifically binds to 5'-phosphorylated single-stranded DNA containing 5'-G and GGGG stretches. The amino acid sequence deduced from the cDNA sequence showed that the S mu bp-2 belongs to the putative helicase superfamily which is involved in replication, recombination and repair. Expression of S mu bp-2 mRNA is ubiquitous and augmented in spleen cells stimulated with lipopolysaccharide and interleukin 4 which also induce class switching. The S mu bp-2 gene is conserved among vertebrates. Possible involvement of S mu bp-2 in class switching is discussed.
Center for Molecular Biology and Genetics, Kyoto University, Japan.
T R
TR
Mizuta
Y
Y
Fukita
T
T
Miyoshi
A
A
Shimizu
T
T
Honjo
D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D004277:DNA, Single-Stranded / Q000737:chemistry / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000302:isolation & purification; D006147:Guanine / Q000032:analysis; D006801:Humans; D007134:Immunoglobulin Switch Region; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D010766:Phosphorylation; D011995:Recombination, Genetic; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors
D004277:DNA, Single-Stranded; D004268:DNA-Binding Proteins; C489892:IGHMBP2 protein, human; C081225:Ighmbp2 protein, mouse; D014157:Transcription Factors; D006147:Guanine