Commercial kits for 1,25-dihydroxyvitamin D compared with a liquid-chromatographic assay.
Three commercially available kits for 1,25-dihydroxyvitamin D [1,25(OH)2D] determination were compared with a liquid-chromatographic method. Serum samples were analyzed for 1,25(OH)2D from 70 healthy subjects (age 2.2-17.5 years; 35 males, 35 females), some (n = 5) given orally high vitamin D3 doses. In addition, 1,25(OH)2D was measured in 28 patients with untreated diseases associated with low (n = 16) or high (n = 12) serum concentrations of the hormone. The results showed that the commercial kits were sufficiently accurate with respect to the comparison method and suitable for routine clinical work.
Department of Pediatrics, University of Pisa, Italy.
S
S
Bertelloni
G I
GI
Baroncelli
U
U
Benedetti
G
G
Franchi
G
G
Saggese
D000293:Adolescent; D002117:Calcitriol / Q000097:blood*; D002648:Child; D002675:Child, Preschool; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D011933:Reagent Kits, Diagnostic / Q000592:standards* / Q000706:statistics & numerical data
D011933:Reagent Kits, Diagnostic; D002117:Calcitriol