Application of kinetic models to estimate transit time through cell cycle compartments.
Models of the carcinogenesis process emphasize the importance of understanding cell cycle-specific effects of a chemical exposure. Development of mathematical models describing the kinetics of individual cell movements within the growth cycle are applied to a cultured cell system. Treatment with 100 micrograms/ml trichloroacetic acid is shown to retard transit through the synthesis phase of the cycle. The models are compared with standard relative movement calculations and are found to be more sensitive. In addition, the DNA compartments are modeled over time to detect possible development of aneuploidy during treatment.
Armstrong Laboratory, Toxic Hazards Division, Wright-Patterson AFB, OH 45433-6573.
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Hancock
D000818:Animals; D002451:Cell Compartmentation / Q000187:drug effects; D002453:Cell Cycle / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002461:Cell Line, Transformed / Q000502:physiology*; D002465:Cell Movement / Q000187:drug effects; D004247:DNA / Q000032:analysis; D007700:Kinetics; D008099:Liver / Q000166:cytology; D008297:Male; D008962:Models, Theoretical; D051381:Rats; D011916:Rats, Inbred F344; D014238:Trichloroacetic Acid / Q000633:toxicity
D014238:Trichloroacetic Acid; D004247:DNA