The Chlamydia trachomatis Mip-like protein is a lipoprotein.
The Mip-like protein of Chlamydia trachomatis is similar to the Mip protein of Legionella pneumophila and may be equally important for the initiation of intracellular infection. This article presents data which identify the chlamydial Mip-like protein as a lipoprotein. The amino acid sequence of the Mip-like protein contains a signal peptidase II recognition sequence, as is seen in procaryotic lipoproteins. Palmitic acid was incorporated into the recombinant chlamydial Mip-like protein. Globomycin, known to inhibit signal peptidase II, inhibited processing of the recombinant Mip-like protein. Labelling of chlamydial organisms with palmitic acid revealed incorporation into the native Mip-like protein.
Microbial Molecular Genetics and Cell Biology Group, School of Biological Sciences, University of Birmingham, United Kingdom.
A G
AG
Lundemose
D A
DA
Rouch
C W
CW
Penn
J H
JH
Pearce
D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000378:metabolism*; D002692:Chlamydia trachomatis / Q000378:metabolism*; D010450:Endopeptidases / Q000187:drug effects; D008074:Lipoproteins / Q000096:biosynthesis*; D008565:Membrane Proteins; D008969:Molecular Sequence Data; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000378:metabolism*; D010455:Peptides / Q000494:pharmacology; D011499:Protein Processing, Post-Translational / Q000187:drug effects; D021382:Protein Sorting Signals; D011994:Recombinant Proteins / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D012697:Serine Endopeptidases
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D008074:Lipoproteins; D008565:Membrane Proteins; D010169:Palmitic Acids; D010455:Peptides; D021382:Protein Sorting Signals; D011994:Recombinant Proteins; C077482:Mip-like protein, Chlamydia trachomatis; D019308:Palmitic Acid; C017339:globomycin; D010450:Endopeptidases; D012697:Serine Endopeptidases; C026515:type I signal peptidase