Chromatographic techniques for the isolation and purification of lipoproteins.
Various modes of chromatography are available for lipoprotein separation. Gel permeation and affinity chromatography are used for preparative purposes and to separate lipoproteins according to size and apolipoprotein content, respectively. Development of rigid supports for gel permeation has led to large improvements in speed and resolution. Reversed-phase high-performance liquid chromatography (HPLC) of apolipoproteins offers the best performance in terms of speed and resolution of structural variants. Due to its high speed and superior resolving power, the recently developed technique of capillary electrophoresis should emerge as an important method for lipoprotein analysis.
Department of Chemistry, McGill University, Montreal, PQ, Canada.
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D002853:Chromatography, Liquid / Q000379:methods; D004586:Electrophoresis / Q000379:methods; D006801:Humans; D008074:Lipoproteins / Q000097:blood / Q000302:isolation & purification*
D008074:Lipoproteins