Characterization of potyviruses from tulip and lily which cause flower-breaking.
Five viruses causing colour-breaking of tulip flowers were isolated from tulips and lilies. Tulip-breaking virus (TBV), tulip top-breaking virus (TTBV), tulip band-breaking virus, Rembrandt tulip-breaking virus and lily mottle virus were all characterized as potyviruses by serology and potyvirus-specific PCR. Sequence analysis of amplified DNA fragments spanning a conserved area of the coat protein cistron of potyviruses was performed in order to classify the isolates as distinct viruses or strains. It appears that all tulip-breaking viruses are distinct viruses and TTBV was found to be strain-related to turnip mosaic virus.
Department of Biochemistry, Leiden University, Gorlaeus Laboratories, The Netherlands.
E L
EL
Dekker
A F
AF
Derks
C J
CJ
Asjes
M E
ME
Lemmers
J F
JF
Bol
S A
SA
Langeveld
D000595:Amino Acid Sequence; D001483:Base Sequence; D004279:DNA, Viral; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases; D010942:Plant Viruses / Q000145:classification / Q000235:genetics*; D010944:Plants / Q000033:anatomy & histology / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D014764:Viral Proteins / Q000235:genetics
D004279:DNA, Viral; D014764:Viral Proteins