Immunologic specificity of IgG against trimellityl-human serum albumin in serum samples of workers exposed to trimellitic anhydride.
The specificity of immunoglobulin G (IgG) against trimellityl-human serum albumin (TM-HSA) in serum samples from 11 workers exposed to trimellitic anhydride (TMA) was characterized in this study. Levels of IgG against TM-HSA and HSA-conjugates of other acid anhydrides, phthalic anhydride (PA), maleic anhydride (MA), hexahydrophthalic anhydride (HHPA) and tetrachlorophthalic anhydride (TCPA) were estimated by enzyme-linked immunosorbent assay index. Inhibition studies using each of 5 HSA acid anhydride conjugates were performed on all 11 serum samples with IgG against TM-HSA, and on the three serum samples with highest IgG binding to P-HSA and M-HSA. The only conjugate capable of inhibiting TM-HSA was TM-HSA. Both P-HSA and TM-HSA were able to inhibit IgG bound to P-HSA, and all other anhydride conjugates were able to inhibit IgG bound to M-HSA to some degree. When using TM-HSA and the other anhydride-HSA conjugates to inhibit IgG against TM-HSA, cross-reactivity was not apparent. However, when using those same conjugates to inhibit IgG against P-HSA or M-HSA, cross-reactivity could be demonstrated in some serum samples. Thus TMA workers may have antibody that has some affinity for other anhydride-HSA conjugates, but this antibody cannot be demonstrated by inhibition studies of IgG against TM-HSA when using other acid anhydride-HSA conjugates. Further studies are needed to define the biologic relevance of these immunologic observations.
Department of Occupational and Environmental Medicine, Lund University, University Hospital, Sweden.
L
L
Gerhardsson
L C
LC
Grammer
M A
MA
Shaughnessy
R
R
Patterson
D000328:Adult; D000918:Antibody Specificity / Q000276:immunology*; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D004853:Epoxy Resins / Q000032:analysis / Q000378:metabolism; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology* / Q000378:metabolism; D008297:Male; D008299:Maleic Anhydrides / Q000032:analysis / Q000378:metabolism; D008875:Middle Aged; D009784:Occupational Diseases / Q000097:blood / Q000276:immunology; D016273:Occupational Exposure; D010796:Phthalic Anhydrides / Q000032:analysis / Q000378:metabolism / Q000494:pharmacology*; D012709:Serum Albumin / Q000032:analysis / Q000276:immunology* / Q000378:metabolism
D004853:Epoxy Resins; D007074:Immunoglobulin G; D008299:Maleic Anhydrides; D010796:Phthalic Anhydrides; D012709:Serum Albumin; C005752:araldite; C009409:tetrachlorophthalic anhydride; C015559:trimellitic anhydride