Renal apolipoproteins in nephrotic rats.
Recent experimental data suggest a role for lipids in the pathogenesis of glomerulosclerosis. In this study, we examined the main apolipoproteins (apo) of high density lipoproteins (A-I, A-IV, E), low density lipoproteins (B), and very low density lipoproteins (B,E) in plasma and kidney tissue of rats with puromycin aminonucleoside or adriamycin nephrosis. In full-blown nephrosis, plasma concentrations of apo A-I and apo B were significantly elevated, apo A-IV and apo E levels did not change. Immunohistological studies in plastic sections revealed increased apo A-I, apo A-IV, and apo E immunoreactivity in glomerular visceral epithelial cells both in puromycin aminonucleoside and adriamycin nephrosis. This was confirmed by immunoelectronmicroscopy. In addition, apo B and apo E were encountered in increased amounts in the mesangium and colocalized with Oil Red O-positive lipid deposits, particularly in puromycin aminonucleoside nephrosis rats. Double-staining showed a preferential localization of apo B and apo E at sites of increased mesangial matrix in close proximity to ED1-positive foam cells, i.e., the mesangial macrophages. The close topographic association between apo B and apo E, lipid deposits, and macrophages in the mesangium lend further support to the concept of lipid-mediated glomerular injury in nephrosis.
Department of Pathology, University of Groningen, The Netherlands.
H
H
van Goor
M L
ML
van der Horst
J
J
Atmosoerodjo
J A
JA
Joles
A
A
van Tol
J
J
Grond
D000818:Animals; D001053:Apolipoproteins / Q000032:analysis* / Q000097:blood / Q000378:metabolism; D001054:Apolipoproteins A / Q000032:analysis; D001055:Apolipoproteins B / Q000097:blood; D001057:Apolipoproteins E / Q000097:blood; D004317:Doxorubicin; D006651:Histocytochemistry; D007668:Kidney / Q000737:chemistry* / Q000378:metabolism / Q000473:pathology; D007678:Kidney Glomerulus / Q000737:chemistry / Q000473:pathology / Q000648:ultrastructure; D008297:Male; D016253:Microscopy, Immunoelectron; D009404:Nephrotic Syndrome / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D011692:Puromycin Aminonucleoside; D051381:Rats; D017208:Rats, Wistar
D001053:Apolipoproteins; D001054:Apolipoproteins A; D001055:Apolipoproteins B; D001057:Apolipoproteins E; D011692:Puromycin Aminonucleoside; D004317:Doxorubicin