Rapid, extensive and reversible vacuolation of Schizosaccharomyces pombe induced by amphotericin B.
The fission yeast Schizosaccharomyces pombe has no large vacuoles under normal growth conditions, although budding yeasts usually have large central vacuoles. The minimum inhibitory concentration of amphotericin B to S. pombe was 0.5 microgram ml-1; treatment with 0.2 microgram ml-1 for 20 min induced rapid and extensive vacuolation in S. pombe exponential phase cells. Growth rate of the cells with 0.2 microgram ml-1 amphotericin B was much reduced for 6 h, showing extensive vacuolation. Vacuolation in itself was not fatal: on removal of the drug, most cells recovered gradually and eventually multiplied.
Department of Fungal Infections, Chiba University, Japan.
K
K
Takeo
K
K
Yarita
S
S
Yoshida
H Q
HQ
Guan
K
K
Nishimura
M
M
Miyaji
D000666:Amphotericin B / Q000494:pharmacology*; D002462:Cell Membrane / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D012568:Schizosaccharomyces / Q000187:drug effects* / Q000254:growth & development / Q000648:ultrastructure; D014617:Vacuoles / Q000187:drug effects*
D000666:Amphotericin B