Determination of ochratoxin A in urine and faeces of swine by high-performance liquid chromatography.
Sensitive methods for the determination of ochratoxin A in urine and faeces of swine are described. The samples were extracted with chloroform at pH < 2, and the extracts were cleaned up by a combination of solid-phase extraction and liquid-liquid partition. High-performance liquid chromatography with fluorescence detection was used for detection and determination. The detection limits were 0.3 ng/ml for urine and 1.5 ng/g for faeces. Recoveries of ochratoxin A from spiked samples of urine and faeces were 93% and 60%, respectively. Because of the low detection limit and the fast and relatively easy performance, the method for the determination of ochratoxin A in urine proved suitable for the estimation of possible contamination of live animals.
Institut für Tierernährung, Bundesforschungsanstalt für Landwirtschaft, Braunschweig, Germany.
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Valenta
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Kühn
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Rohr
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000737:chemistry*; D007202:Indicators and Reagents; D009793:Ochratoxins / Q000032:analysis* / Q000652:urine; D013050:Spectrometry, Fluorescence; D013552:Swine
D007202:Indicators and Reagents; D009793:Ochratoxins; C025589:ochratoxin A