Calvasculin, as a factor affecting the microfilament assemblies in rat fibroblasts transfected by src gene.
Cell transformations accompany alterations in cell morphology and microfilament patterns. Calvasculin encodes mRNA termed pEL-98, 18A2, 42A, p9Ka, or mts1, found to be elevated in several metastatic cell lines. We report the elevation of calvasculin expression in SR-3Y1 cells, which show disappearance of ordered microfilaments, compared to that in 3Y1 cells and that the similar distribution of calvasculin to that of actin filaments. Interestingly, calvasculin co-sediments with F-actin and bundles actin filaments in a Ca(2+)-dependent manner. This activity, along with the elevation of calvasculin following transformation, suggests that the disorganization of filaments in SR-3Y1 cell is due to the cross-linking activity of calvasculin.
Department of Pharmacology, Nagoya University School of Medicine, Japan.
Y
Y
Watanabe
N
N
Usada
H
H
Minami
T
T
Morita
S
S
Tsugane
R
R
Ishikawa
K
K
Kohama
Y
Y
Tomida
H
H
Hidaka
D008841:Actin Cytoskeleton / Q000378:metabolism / Q000648:ultrastructure*; D000199:Actins / Q000302:isolation & purification / Q000378:metabolism* / Q000648:ultrastructure; D000818:Animals; D002135:Calcium-Binding Proteins / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002645:Chickens; D005347:Fibroblasts / Q000378:metabolism / Q000648:ultrastructure; D016391:Genes, src; D008854:Microscopy, Electron; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D000071999:S100 Calcium-Binding Protein A4; D009418:S100 Proteins; D014162:Transfection
D000199:Actins; D002135:Calcium-Binding Proteins; D012333:RNA, Messenger; D000071999:S100 Calcium-Binding Protein A4; D009418:S100 Proteins; C499412:S100a4 protein, rat