Fractionation of erythroblasts with affinity-mediated modifications of their electrical properties using counter-current distribution.
We have previously reported the possibility of modifying the electrical properties of cells by means of their interaction with a specific ligand carrying a polyelectrolyte (Anal Biochem 200: 280-285). This selective modification of receptor-containing cells changed their partition in a charge-sensitive aqueous two-phase system. We here present the fractionation of electrically modified erythroblasts by the use of an automatic multiple-partition procedure, counter-current distribution. The cells were fractionated according to the degree of differentiation of erythroblasts as evaluated from the hemoglobin content as well as the relative activities of the two enzymes, 3-phosphoglycerate kinase and bisphospho-glycerate mutase.
Department of Biochemistry, University of Lund, Sweden.
J
J
Mendieta
G
G
Johansson
D000818:Animals; D001731:Bisphosphoglycerate Mutase / Q000032:analysis; D002454:Cell Differentiation; D002469:Cell Separation / Q000379:methods*; D003377:Countercurrent Distribution; D004900:Erythroblasts / Q000737:chemistry* / Q000187:drug effects; D008297:Male; D010735:Phosphoglycerate Kinase / Q000032:analysis; D011107:Polylysine / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D013499:Surface Properties / Q000187:drug effects; D014168:Transferrin / Q000494:pharmacology
D014168:Transferrin; D011107:Polylysine; D010735:Phosphoglycerate Kinase; D001731:Bisphosphoglycerate Mutase