Analysis of the petunia nitrate reductase apoenzyme-encoding gene: a first step for sequence modification analysis.
In this paper, we describe the gene (nia) coding for the apoenzyme of the nitrate reductase (NR) of petunia. A full-size genomic clone was isolated from a genomic library, using the tobacco nia2 cDNA as a probe, and sequenced. The open reading frame is interrupted by three introns and encodes a protein of 909 amino acids which reveals between 92% and 68% identity to the NADH NR apoenzyme from other higher plants. Southern analyses indicated that the NR apoenzyme is encoded by a single-copy gene, although another region homologous to part of nia was also identified. The analysis of the steady-state level of nia mRNA showed that the petunia nia is regulated by the nitrogen source and is under the control of the circadian rhythm.
Salanoubat
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Laboratoire de Génétique Végétale, Université Paris-Sud, CNRS, Orsay, France.
Bui Dang Ha
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D000595:Amino Acid Sequence; D001051:Apoenzymes / Q000235:genetics*; D001483:Base Sequence; D002940:Circadian Rhythm; D003001:Cloning, Molecular; D004252:DNA Mutational Analysis; D015971:Gene Expression Regulation, Enzymologic; D017343:Genes, Plant; D008969:Molecular Sequence Data; D050901:Nitrate Reductase; D009565:Nitrate Reductases / Q000235:genetics*; D009584:Nitrogen / Q000378:metabolism; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000032:analysis; D012689:Sequence Homology, Nucleic Acid
D001051:Apoenzymes; D012333:RNA, Messenger; D009565:Nitrate Reductases; D050901:Nitrate Reductase; D009584:Nitrogen