Oestradiol measurement in women on oral hormone replacement therapy: the validity of commercial test kits.
Four commercially available methods for the measurement of oestradiol were used to assay oestradiol concentrations in samples from women receiving oral hormone replacement therapy. Significant differences in results were found between samples that were measured direct and those subjected to an extraction procedure. There was considerable variability in the extraction: direct ratio between methods, indicating that with some methods an extraction procedure should be performed before analysis on samples from women receiving oral hormone replacement therapy. Factors that account for discrepant results between methods probably include increased concentration of sex hormone binding globulin together with cross-reacting metabolites and conjugates.
Steroid Assay Service Laboratory, University Hospital of Wales, Heath Park, Cardiff, UK.
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D000284:Administration, Oral; D004958:Estradiol / Q000032:analysis* / Q000627:therapeutic use; D015914:Estrogen Replacement Therapy; D005260:Female; D006801:Humans; D011933:Reagent Kits, Diagnostic / Q000592:standards*
D011933:Reagent Kits, Diagnostic; D004958:Estradiol