Studies of the protein synthesis system in the brain cortex during global ischemia and reperfusion.
Previous studies have demonstrated that brain protein synthesis declines after global ischemia and reperfusion. To investigate the role of the translation system in this phenomenon, we examined the ability of partially purified ribosomes, ribosome-bound mRNA and translation cofactors derived from the transiently ischemic cerebral cortex to synthesize protein in vitro. Samples were prepared from canines subjected to 20-min cardiac arrest and after 2 or 8 h of post-resuscitation intensive care. There was no significant decrease in the rate of in vitro protein synthesis as a consequence of either ischemia or reperfusion. Northern hybridization of ribosome-bound RNA revealed a discrete band of mRNA for brain-specific creatine kinase (ck-bb) that was consistent in presence and intensity in all groups. However, mRNA for heat shock 70 protein (hsp-70) was observed only during reperfusion and markedly increased between 2 and 8 h reperfusion. Thus, we conclude that (1) the transcription system is intact during reperfusion and hsp-70 mRNA is made and translocated to the ribosomes during reperfusion, (2) mRNA for ck-bb is not displaced from ribosomes by the appearance of hsp-70 during reperfusion and (3) isolated ribosomes maintain their ability to translate in vitro during the first 8 h of reperfusion after global brain ischemia. Therefore, the early reduction in protein synthesis observed in vivo during post-ischemic brain reperfusion is not due to an intrinsic dysfunction of the ribosomes.
Department of Emergency Medicine, Wayne State University School of Medicine, Detroit, MI 48201.
D J
DJ
DeGracia
B J
BJ
O'Neil
C
C
Frisch
G S
GS
Krause
J M
JM
Skjaerlund
B C
BC
White
L I
LI
Grossman
D000818:Animals; D015152:Blotting, Northern; D002545:Brain Ischemia / Q000235:genetics / Q000378:metabolism*; D002540:Cerebral Cortex / Q000378:metabolism*; D003402:Creatine Kinase / Q000096:biosynthesis* / Q000235:genetics; D004285:Dogs; D006323:Heart Arrest / Q000628:therapy; D006360:Heat-Shock Proteins / Q000096:biosynthesis* / Q000235:genetics; D007527:Isoenzymes; D014176:Protein Biosynthesis / Q000502:physiology; D012333:RNA, Messenger / Q000096:biosynthesis*; D015427:Reperfusion Injury / Q000235:genetics / Q000378:metabolism*; D012151:Resuscitation; D012270:Ribosomes / Q000378:metabolism; D014158:Transcription, Genetic / Q000502:physiology
D006360:Heat-Shock Proteins; D007527:Isoenzymes; D012333:RNA, Messenger; D003402:Creatine Kinase