[Development of an enzyme immunoassay for the detection of antibodies from hens and mice against Newcastle disease virus].
The development of an enzyme-immuno-assay (EIA) for detection of avian and mouse antibodies against the Newcastle-Disease Virus is shown. The antigen preparation was the centre of the experiments. The basic assay programme and selected test steps were optimized. The established assay is part of the EIA-control system for a SPF (specific-pathogen-free)-flock and also the essential screening method for the production of anti-NDV-monoclonal antibodies.
Institut für Virologie und Geflügelkrankheiten, Veterinärmedizinischen Fakultät, Humboldt-Universität zu Berlin.
P
P
Minning
A
A
Hlinak
K
K
Klaczinski
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D000956:Antigens, Viral / Q000276:immunology; D001667:Binding, Competitive; D002642:Chick Embryo; D002645:Chickens; D015925:Cryopreservation; D005260:Female; D005612:Freeze Drying; D007124:Immunoenzyme Techniques / Q000662:veterinary*; D051379:Mice; D009521:Newcastle Disease / Q000276:immunology*; D009522:Newcastle disease virus / Q000276:immunology*
D000914:Antibodies, Viral; D000956:Antigens, Viral
Entwicklung eines Enzym-Immuno-Assay zum Nachweis von Antikörpern des Huhnes und der Maus gegen das Virus der Newcastle-Disease.