Comparative study of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and tritiated thymidine in a chemosensitivity test using collagen gel matrix.
An organ culture system using collagen gel matrix (CGM) was modified and established successfully as a rapid and convenient method for determination of anti-cancer drug sensitivities. The cell viability of a tumor fragment was measured by the reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) to a colored formazan product, which allowed for quantitative and simple analysis. The sensitivities of KK-47 bladder tumor from nude mice to various anti-cancer drugs tested corresponded closely to those determined in the original CGM assay system using tritiated thymidine, which has a high clinical correlation. Our modified method can be used as a practical and highly reproducible chemosensitivity test in vitro.
Department of Urology, School of Medicine, Kanazawa University, Japan.
M
M
Egawa
H
H
Hisazumi
T
T
Uchibayashi
M
M
Tanaka
T
T
Sasaki
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D002295:Carcinoma, Transitional Cell / Q000188:drug therapy* / Q000473:pathology; D003094:Collagen; D004396:Coloring Agents; D004353:Drug Evaluation, Preclinical / Q000379:methods* / Q000706:statistics & numerical data; D005782:Gels; D006801:Humans; D051379:Mice; D009924:Organ Culture Techniques; D012680:Sensitivity and Specificity; D013778:Tetrazolium Salts; D013844:Thiazoles; D013936:Thymidine; D014316:Tritium; D001749:Urinary Bladder Neoplasms / Q000188:drug therapy* / Q000473:pathology
D000970:Antineoplastic Agents; D004396:Coloring Agents; D005782:Gels; D013778:Tetrazolium Salts; D013844:Thiazoles; D014316:Tritium; D003094:Collagen; C022616:thiazolyl blue; D013936:Thymidine