[Genetic engineering of human insulin. II. Exclusion HPLC of biotechnological precursors. Factors affecting resolution and selectivity].
Mechanisms of the exclusion-sorption interaction of insulin-containing proteins with the column support in accordance with the chemical and three-dimensional structure were considered. The weak adsorption of the linear proinsulin and fusion protein in non-denaturing conditions, and dynamics of the SDS-protein complex's formation in denaturing conditions were studied. The obtained results are used for SE HPLC analysis of the main products and intermediates at essentially all steps of the recombinant human insulin production.
Kliushnichenko
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Vul'son
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D016477:Artifacts; D001709:Biotechnology; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D005818:Genetic Engineering; D006801:Humans; D007328:Insulin / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics*; D011384:Proinsulin / Q000302:isolation & purification; D011498:Protein Precursors / Q000302:isolation & purification*; D011993:Recombinant Fusion Proteins / Q000302:isolation & purification; D011994:Recombinant Proteins / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics
D007328:Insulin; D011498:Protein Precursors; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; D011384:Proinsulin
Genno-inzhenernyĭ insulin cheloveka. II. Ekskliuzionnaia VEZhKh biotekhnologicheskikh predshestvennikov. Faktory, vliiaiushchie na razreshenie i selektivnost'.