Cauliflower mosaic virus 35S promoter-controlled DNA copies of cowpea mosaic virus RNAs are infectious on plants.
Clones have been constructed that contain full-length cDNA copies of cowpea mosaic virus RNA1 and RNA2, downstream of the cauliflower mosaic virus 35S promoter. The clones, when linearized downstream of the viral sequences, give rise to cowpea mosaic virus-like symptoms when inoculated onto cowpea plants. Viral RNA and virions can be detected in the inoculated plants, demonstrating that the clones are directly infectious.
Department of Virus Research, John Innes Institute, John Innes Centre, Norwich, U.K.
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Dessens
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Lomonossoff
D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D004279:DNA, Viral; D008969:Molecular Sequence Data; D009029:Mosaic Viruses / Q000235:genetics* / Q000472:pathogenicity; D010944:Plants / Q000382:microbiology*; D011401:Promoter Regions, Genetic; D012367:RNA, Viral / Q000235:genetics*; D015183:Restriction Mapping
D004279:DNA, Viral; D012367:RNA, Viral