N-3-hydroxypropionyl-alpha-D-perosamine homopolymer constituting the O-chain of lipopolysaccharides from Vibrio bioserogroup 1875 possessing antigenic factor(s) in common with O1 Vibrio cholerae.
A structural study was performed by 13C-n.m.r. spectroscopy and methylation analysis of the O-chain of lipopolysaccharide (LPS) from Vibrio bioserogroup 1875 possessing antigenic factor(s) in common with O1 Vibrio cholerae. It was demonstrated to contain a linear homopolymer of (1-->2)-linked N-3-hydroxypropionyl-alpha-D-perosamine [4-(3-hydroxypropanamido)-4,6-dideoxy-alpha-D-mannopyranose], which is very similar to, but not identical with, both (1-->2)-linked linear N-3-deoxy-L-glycero-tetronyl(S-2,4-dihydroxybutyryl)-alpha-D - perosamine homopolymer and (1-->2)-linked linear N-acetyl-alpha-D-perosamine homopolymer which constitute the O-chains of O1 V. cholerae and non-O1 V. cholerae bioserogroup Hakata LPS respectively.
Department of Microbiology, School of Pharmaceutical Sciences, Josai University, Saitama, Japan.
S
S
Kondo
K
K
Ishida
Y
Y
Isshiki
Y
Y
Haishima
T
T
Iguchi
K
K
Hisatsune
D000107:Acetylation; D000606:Amino Sugars / Q000032:analysis*; D000942:Antigens, Bacterial / Q000737:chemistry*; D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000032:analysis; D008401:Gas Chromatography-Mass Spectrometry; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000276:immunology; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D011108:Polymers; D011134:Polysaccharides / Q000032:analysis*; D014734:Vibrio cholerae / Q000737:chemistry*
D000606:Amino Sugars; D000942:Antigens, Bacterial; D002241:Carbohydrates; D008070:Lipopolysaccharides; D011108:Polymers; D011134:Polysaccharides; C069468:N-acetylperosamine