Construction of a Chlamydomonas reinhardtii mutant with an intronless psbA gene.
Efficient chloroplast transformation systems now available allow the manipulation of the evolutionarily highly conserved psbA gene in the eucaryotic organism Chlamydomonas reinhardtii. Two copies of this gene in the inverted repeat region of the chloroplast genome contain four large group I introns. To analyse possible functions of these introns and to generate a mutant for simplified psbA gene manipulations, a psbA cDNA fragment was introduced into a psbA deletion mutant using the biolistic transformation method. A transformant with no introns in the psbA gene has been obtained and represents the first example of the removal of a complete set of introns from a chloroplast gene. The newly generated strain is photosynthetically competent and contains no detectable recipient genome copies. The loss of all four introns appears to be phenotypically silent.
Ruhr-Universität Bochum, Lehrstuhl für Biochemie der Pflanzen, FRG.
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Johanningmeier
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Heiss
D000818:Animals; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics*; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D015153:Blotting, Western; D016825:Chlamydomonas reinhardtii / Q000235:genetics*; D004579:Electron Transport; D007438:Introns / Q000235:genetics*; D008969:Molecular Sequence Data; D010788:Photosynthesis / Q000235:genetics; D045332:Photosystem II Protein Complex; D010940:Plant Proteins / Q000096:biosynthesis* / Q000235:genetics*; D016133:Polymerase Chain Reaction; D014170:Transformation, Genetic
D001426:Bacterial Proteins; D045332:Photosystem II Protein Complex; D010940:Plant Proteins