p-Bromophenacyl bromide potentiates hydrogen peroxide formation in human platelets challenged by thrombin and inhibits aggregation.
p-Bromophenacyl bromide, a potent alkylating agent inhibitor of phospholipase A2 and adenylate cyclase, potentiates hydrogen peroxide production in human platelets activated with thrombin. The maximal cooperative effect, specific for thrombin as inducer and potentiated by exogenous calcium, was observed at 2 microM BPB. Since it was shown that BPB is also a strong inhibitor of platelet aggregation (IC 50 = 3 +/- 1 microM) it is likely to suppose that the abnormal accumulation of hydrogen peroxide can influence platelet function.
Istituto Policattedra di Chimica Biologica, Università di Genova, Italy.
G
G
Leoncini
B
B
Aprile
M
M
Maresca
D000098:Acetophenones / Q000494:pharmacology*; D001792:Blood Platelets / Q000187:drug effects* / Q000378:metabolism; D004357:Drug Synergism; D006801:Humans; D006861:Hydrogen Peroxide / Q000097:blood*; D010741:Phospholipases A / Q000037:antagonists & inhibitors*; D054467:Phospholipases A2; D015539:Platelet Activation; D010974:Platelet Aggregation / Q000187:drug effects; D013050:Spectrometry, Fluorescence; D013917:Thrombin / Q000494:pharmacology
D000098:Acetophenones; D006861:Hydrogen Peroxide; D010741:Phospholipases A; D054467:Phospholipases A2; D013917:Thrombin; C006821:4-bromophenacyl bromide