Morphofunctional characterization of lymphokine activated killer (LAK) cells in vitro.
In order to characterize the cellular subsets of peripheral blood lymphocytes cultured with interleukin-2 (IL-2), a study in vitro was developed. Both conditioned medium from PHA-stimulated lymphocytes and recombinant IL-2 (rIL-2) were employed. The following aspects were examined: the morphological pattern of IL-2 stimulated lymphocytes; the ability of these cells to recognize, bind, attack and destroy tumoral lines; their cytochemical and immunological (CD) feature. The results suggest that, in our experimental conditions, these cells showed a characteristic "hand mirror shape", cytotoxic antitumor activity, lack of hydrolytic enzymes and CD3+, CD4+ phenotype.
Department of Animal Biology, University of Pavia.
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R
Nano
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Capelli
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Gerzeli
D002478:Cells, Cultured; D017077:Culture Media, Conditioned / Q000494:pharmacology; D003602:Cytotoxicity, Immunologic; D006801:Humans; D016130:Immunophenotyping; D007376:Interleukin-2 / Q000494:pharmacology; D015979:Killer Cells, Lymphokine-Activated / Q000187:drug effects / Q000276:immunology / Q000648:ultrastructure*; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000378:metabolism; D011994:Recombinant Proteins / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D017077:Culture Media, Conditioned; D007376:Interleukin-2; D011994:Recombinant Proteins