Two-site comparison of broth microdilution and semisolid agar dilution methods for susceptibility testing of Cryptococcus neoformans in three media.
This study evaluated the inter- and intralaboratory agreement between results of the semisolid agar dilution and broth microdilution methods of antifungal susceptibility testing of Cryptococcus neoformans. Three media were tested in two laboratories. The drugs tested were amphotericin B, flucytosine, itraconazole, fluconazole, and Schering 39304. Analysis by kappa statistics revealed good agreement between the laboratories for the two methods. The highest level of inter- and intralaboratory agreement was observed in RPMI 1640 with L-glutamine followed by Eagle's minimum essential medium and yeast nitrogen broth. The broth microdilution method appears more suitable than the semisolid agar dilution method for testing cryptococci because of its ease in performance, cost, and simplicity.
Anaissie
E
E
Department of Infectious Diseases, University of Texas M.D. Anderson Cancer Center, Houston 77030.
Shawar
R
R
Paetznick
V
V
Ensign
L G
LG
Witte
Z
Z
LaRocco
M
M
D000362:Agar; D000935:Antifungal Agents / Q000494:pharmacology; D003453:Cryptococcosis / Q000188:drug therapy; D003455:Cryptococcus neoformans / Q000187:drug effects* / Q000302:isolation & purification; D003470:Culture Media; D005069:Evaluation Studies as Topic; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods* / Q000706:statistics & numerical data; D015203:Reproducibility of Results
D000935:Antifungal Agents; D003470:Culture Media; D000362:Agar