Cross-reactivity between Borrelia burgdorferi flagellin and a human axonal 64,000 molecular weight protein.
The serum of patients with Lyme neurologic disease contain antibodies that bind to human axonal antigens that cross-react with Borrelia burgdorferi. The sera also bind to SK-N-SH neuroblastoma cells, especially the neuritic processes of these cells. H9724, a murine IgG monoclonal antibody to B. burgdorferi flagellin, binds to an SK-N-SH cell protein of approximately 64,000 apparent molecular weight (M(r)). H9724 immunoprecipitates a protein of the same M(r) (p64) from the cells and from a delipidated preparation of human peripheral nerve. The Lyme disease patient sera that bind to human axons and SK-N-SH cells also bind to the immunoprecipitated p64. Immunologic cross-reactivity between borrelial and human axonal proteins may be involved in the immunopathogenesis of Lyme neurologic disease.
Department of Medicine, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, New Brunswick 08903-0019.
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D000818:Animals; D001369:Axons / Q000276:immunology*; D015153:Blotting, Western; D015748:Borrelia burgdorferi Group / Q000276:immunology*; D003429:Cross Reactions; D005408:Flagellin / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D006801:Humans; D008193:Lyme Disease / Q000276:immunology*; D051379:Mice; D009419:Nerve Tissue Proteins / Q000276:immunology*; D051381:Rats; D014407:Tumor Cells, Cultured
D009419:Nerve Tissue Proteins; D005408:Flagellin