[DNAse-I inhibition by the chromatin proteins of the brain cells in rats].
The effect of protein extracted from nuclei of brain cells on the reaction of DNA-ethidium bromide complex interaction with DNAase I and Bac. subtilis nuclease was studied. The nuclei were isolated from the brain of Wistar male rats weighting 180-200 g. The fluorescence was recorded on a Hitachi MPF-44B spectrofluorimeter. Proteins extracted from the nuclei with 0.6 NaCl substantially inhibited the DNAase I but not Bac. subtilis nuclease activity. Other studied fractions of nuclei lacked the capacity for inhibiting DNAase I.
Smirnova
G N
GN
Tret'iak
T M
TM
Terpilovskaia
O N
ON
D000818:Animals; D001412:Bacillus subtilis / Q000187:drug effects / Q000201:enzymology; D001921:Brain / Q000502:physiology*; D003850:Deoxyribonuclease I / Q000037:antagonists & inhibitors* / Q000187:drug effects; D004996:Ethidium / Q000378:metabolism; D008297:Male; D009419:Nerve Tissue Proteins / Q000302:isolation & purification / Q000494:pharmacology*; D009562:Nissl Bodies / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D013050:Spectrometry, Fluorescence; D013379:Substrate Specificity
D009419:Nerve Tissue Proteins; D003850:Deoxyribonuclease I; D004996:Ethidium
Ingibirovanie DNKazy-I belkami khromatina kletok golovnogo mozga krys.