A simple and rapid method for purification of oligodeoxyribonucleoside methylphosphonates.
An alternative dimethoxytrityl-on (dmt-on) method is described to purify hydrophobic oligodeoxyribonucleoside methyl-phosphonates (OM) with a phosphodiester linkage at the 5' end, instead of the conventional dmt-off method using a DEAE ion-exchange column. This method is modified from the reverse-phase method for purification of normal oligonucleotides.
Lin
S B
SB
Department of Clinical Pathology, College of Medicine, National Taiwan University, Republic of China.
Chang
G W
GW
Teh
G W
GW
Lin
K I
KI
Au
L C
LC
D001483:Base Sequence; D001709:Biotechnology; D002848:Chromatography, DEAE-Cellulose; D016373:DNA, Antisense / Q000737:chemistry / Q000302:isolation & purification; D005029:Ethylenediamines; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000737:chemistry / Q000302:isolation & purification*; D063065:Organophosphonates / Q000302:isolation & purification
D016373:DNA, Antisense; D005029:Ethylenediamines; D009838:Oligodeoxyribonucleotides; D063065:Organophosphonates; C031234:ethylenediamine