Separation of rooster spermatogenic nuclei by means of centrifugal elutriation.
By application of minor variations in the usual method of centrifugal elutriation, we have been able to separate a nuclear suspension from rooster testes. We have obtained a population of elongated or late spermatid nuclei (98% purity), a population of round or early spermatid nuclei (90% purity), and, finally, a mixed population of meiotic and pre-meiotic nuclei.
Department of Physiological Sciences, University of Barcelona School of Medicine, Spain.
J
J
Boix
J
J
Roca
D000818:Animals; D002458:Cell Fractionation / Q000379:methods*; D002467:Cell Nucleus; D002499:Centrifugation, Density Gradient / Q000379:methods*; D002645:Chickens; D004247:DNA / Q000032:analysis; D008297:Male; D008540:Meiosis; D015203:Reproducibility of Results; D013087:Spermatids / Q000648:ultrastructure*; D013091:Spermatogenesis
D004247:DNA