Differential regulation of group A streptococcal peptidoglycan-polysaccharide (PG-APS)-stimulated macrophage production of IL-1 by rat strains susceptible and resistant to PG-APS-induced arthritis.
The magnitude of the response of rat macrophages to group A streptococcal cell wall peptidoglycan-polysaccharide (PG-APS) stimulation is strain-dependent. A comparison of IL-1 expression between macrophages from a PG-APS-resistant (BUF) and PG-APS-sensitive (LEW) rat strain revealed that while mRNA levels for IL-1 alpha and IL-1 beta were equivalent for the two strains, supernatants from BUF macrophages were less potent than LEW supernatants at stimulating thymocyte proliferation and contained less immunoreactive IL-1 alpha and IL-1 beta protein. BUF and LEW macrophages expressed nearly equivalent levels of IL-1 receptor antagonist (IL-1ra) mRNA and secreted IL-1ra protein in supernatants. The ratio of IL-1/IL-1ra produced by macrophages may influence susceptibility versus resistance to PG-APS and is strongly suggestive of a regulatory role for macrophages in the pathogenesis of PG-APS-induced arthritis.
Laboratory of Physiologic and Pharmacologic Studies, National Institute on Alcoholism and Alcohol Abuse, ADAMHA, National Institutes of Health, Rockville, Maryland 20852.
L A
LA
Bristol
S K
SK
Durum
S P
SP
Eisenberg
D000818:Animals; D001169:Arthritis, Experimental / Q000276:immunology*; D005260:Female; D015870:Gene Expression; D066298:In Vitro Techniques; D053590:Interleukin 1 Receptor Antagonist Protein; D007375:Interleukin-1 / Q000096:biosynthesis*; D008213:Lymphocyte Activation; D008262:Macrophage Activation; D008264:Macrophages / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D008809:Mice, Inbred C3H; D010457:Peptidoglycan / Q000276:immunology*; D011135:Polysaccharides, Bacterial / Q000276:immunology*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D011915:Rats, Inbred BUF; D011917:Rats, Inbred Lew; D012795:Sialoglycoproteins / Q000378:metabolism; D013297:Streptococcus pyogenes / Q000276:immunology*
C507764:Il1rn protein, mouse; D053590:Interleukin 1 Receptor Antagonist Protein; D007375:Interleukin-1; D010457:Peptidoglycan; D011135:Polysaccharides, Bacterial; D012333:RNA, Messenger; D012795:Sialoglycoproteins