Monoclonal antibodies against human hemopexin and their application.
Six monoclonal antibodies raised against human serum hemopexin have been characterized. The antibodies reacted with serum hemopexin as well as with the isolated protein in apo-form and with heme-protein complexes on immunoblots obtained following both PAGE and SDS-PAGE. In the case of PAGE blots 1 ng of hemopexin could be detected using a streptavidin-AP detection system. ELISA procedures employing two different pairs of monoclonal antibodies gave working ranges of 0.3-3 mg/l and 10-100 micrograms/l respectively.
Institute of Hematology and Blood Transfusion, Prague, Czech Republic.
Z
Z
Hrkal
H
H
Cajthamlová
I
I
Kalousek
J
J
Bartosová
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006466:Hemopexin / Q000032:analysis* / Q000276:immunology; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C
D000911:Antibodies, Monoclonal; D006466:Hemopexin