Expression of mRNA for the GATA-binding proteins in human eosinophils and basophils: potential role in gene transcription.
The expression of the hematopoietic transcription factors GATA-1, GATA-2, and GATA-3 was studied in eosinophils and basophils. Eosinophils express mRNA for GATA-1, GATA-2, and GATA-3. Basophils express GATA-2 and GATA-3. Treatment of HL-60 eosinophilic sublines with either interleukin-5 or butyric acid increased the expression of GATA-1 mRNA concomitant with the expression of eosinophil-specific genes, whereas levels of GATA-2 mRNA remained relatively constant. The presence of mRNA for these proteins in eosinophils and basophils suggests that gene transcription in these lineages may be regulated by GATA-binding proteins.
Zon
L I
LI
Division of Hematology, Children's Hospital, Boston, MA 02115.
Yamaguchi
Y
Y
Yee
K
K
Albee
E A
EA
Kimura
A
A
Bennett
J C
JC
Orkin
S H
SH
Ackerman
S J
SJ
D001483:Base Sequence; D001491:Basophils / Q000378:metabolism*; D015152:Blotting, Northern; D002087:Butyrates / Q000494:pharmacology; D020148:Butyric Acid; D002460:Cell Line; D004268:DNA-Binding Proteins / Q000235:genetics*; D004804:Eosinophils / Q000378:metabolism*; D050981:Erythroid-Specific DNA-Binding Factors; D050982:GATA1 Transcription Factor; D050989:GATA2 Transcription Factor; D050990:GATA3 Transcription Factor; D015870:Gene Expression / Q000187:drug effects; D006367:HeLa Cells; D006801:Humans; D015848:Interleukin-5 / Q000494:pharmacology; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000096:biosynthesis*; D015534:Trans-Activators / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*; D014158:Transcription, Genetic
D002087:Butyrates; D004268:DNA-Binding Proteins; D050981:Erythroid-Specific DNA-Binding Factors; D050982:GATA1 Transcription Factor; C494688:GATA1 protein, human; D050989:GATA2 Transcription Factor; C494711:GATA2 protein, human; D050990:GATA3 Transcription Factor; C494749:GATA3 protein, human; D015848:Interleukin-5; D012333:RNA, Messenger; D015534:Trans-Activators; D014157:Transcription Factors; D020148:Butyric Acid