A simple chemical method for the oxidation of beta-hydroxybutyrate; application of the method for the estimation of ketone bodies.
A simple one-step method employing potassium persulphate as an oxidising agent in presence of catalyst, Ag2+, for the oxidation of beta-hydroxybutyrate (beta-OHB) to acetoacetate (AcAc) has been developed and standardized. Under the condition of assay, beta-OHB (0.079-0.395 microM) was quantitatively transformed to AcAc. The reaction linearity was observed from 0.079 to 0.634 microM. Optimum conditions were: pH, 6.2; temp., 40 degrees C; persulphate saturation, 40% and catalyst, 1.82 mM. Under the experimental condition, no reversal of inhibition caused by chloride (22.96 mM) was observed at Ag+ concentrations (9.09 and 18.18 mM), while higher conc. of Ag+ (27.27 mM) caused significant reversal of inhibition (about 60%). The maximum reversal of inhibition was achieved at Ag+ (36.36 mM). The level of ketone bodies, when estimated by the present method, was greatly enhanced during starvation period and about 2- and 12-fold higher level of ketone bodies was observed (compared to control) in rats fasted for 24 and 48 hr respectively.
National Institute of Occupational Health (ICMR), Meghani Nagar, Ahmedabad, India.
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Zaidi
D020155:3-Hydroxybutyric Acid; D000818:Animals; D006885:Hydroxybutyrates / Q000737:chemistry*; D007657:Ketone Bodies / Q000097:blood*; D008297:Male; D010084:Oxidation-Reduction; D011188:Potassium; D017680:Potassium Compounds; D051381:Rats; D012834:Silver; D013431:Sulfates
D006885:Hydroxybutyrates; D007657:Ketone Bodies; D017680:Potassium Compounds; D013431:Sulfates; D012834:Silver; C009007:potassium persulfate; D011188:Potassium; D020155:3-Hydroxybutyric Acid