The effect of the male contraceptive agent gossypol acetic acid on mouse bone marrow cells in vivo: micronuclei and mitotic index.
In this work the genotoxic effect of gossypol acetic acid (gossypol) was evaluated by determining the frequency of micronuclei and mitotic index in male mouse bone marrow cells in vivo. Bone marrow cells were collected at 24th hour after the single intraperitoneal (20, 40, and 80 micrograms/g) administration of gossypol. Polychromatic erythrocytes (PCEs) in the bone marrow were then evaluated with respect to micronuclei frequency. The dose-dependent increase in the micronuclei frequency was observed. However, when compared with the control group, the increase was not found to be significant (P > 0.05). Also the mitotic index values were not found to be different from those control values (P > 0.05). The results suggest that gossypol is not a clastogenic and mutagenic agent in mouse bone marrow cells in vivo.
Department of Medical Biology and Genetics, Faculty of Medicine Gazi University, Ankara, Turkey.
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Orhan
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Ekmekçi
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Menevşe
D000818:Animals; D001853:Bone Marrow / Q000187:drug effects*; D004249:DNA Damage; D004305:Dose-Response Relationship, Drug; D006072:Gossypol / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000494:pharmacology; D006801:Humans; D007274:Injections, Intraperitoneal; D008297:Male; D051379:Mice; D048629:Micronuclei, Chromosome-Defective / Q000187:drug effects*; D015162:Micronucleus Tests; D008938:Mitosis / Q000187:drug effects; D008940:Mitotic Index / Q000187:drug effects*; D013089:Spermatocidal Agents / Q000008:administration & dosage / Q000494:pharmacology*
D013089:Spermatocidal Agents; D006072:Gossypol; C028178:gossypol acetic acid