Increased clearance of Candida albicans from the peritoneal cavity of mice pretreated with concanavalin A or jacalin.
We have studied the role of polymorphonuclear leukocytes and macrophages in the clearance of Candida albicans from the peritoneal cavity of Swiss mice after treatment with jacalin or concanavalin A (Con-A). Mice (25-30 g, N = 7 per group) received jacalin or Con-A (500 micrograms/0.5 ml PBS) intraperitoneally 96 h before intraperitoneal inoculation of 5 x 10(7) yeast cells. The clearance of Candida from the peritoneal cavity was complete 24 h after inoculation for animals pretreated with jacalin and 48 h after inoculation for animals pretreated with Con-A, whereas a reduction to 4 x 10(4) yeast cells/cavity occurred in control animals 48 h after inoculation. Pretreatment with jacalin or Con-A reduced the recovery of C. albicans from spleen, kidney and liver 10- to 80-fold compared to control animals. Pretreatment with the lectins increased the number of phagocytic cells in the peritoneal exudate 5- to 10-fold and their candidacidal activity was increased 6-fold compared to controls. These data explain the increased rate of clearance and reduced yeast dissemination to the viscera of lectin treated mice.
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Departamento de Patologia Geral, Universidade Estadual de Londrina, Brasil.
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D000818:Animals; D002176:Candida albicans / Q000187:drug effects* / Q000276:immunology; D003208:Concanavalin A / Q000494:pharmacology*; D007369:Interferon Inducers / Q000494:pharmacology*; D037102:Lectins / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects / Q000502:physiology; D051379:Mice; D009504:Neutrophils / Q000187:drug effects / Q000502:physiology; D010587:Phagocytosis / Q000187:drug effects; D037121:Plant Lectins; D013997:Time Factors
D007369:Interferon Inducers; D037102:Lectins; D037121:Plant Lectins; C043696:jacalin; D003208:Concanavalin A