Quantitative analysis of cefradine by liquid chromatography on poly(styrene-divinylbenzene).
A method is described for isocratic analysis of cefradine by liquid chromatography on a poly(styrene-divinylbenzene) column (PLRP-S, 250 x 4.6 mm i.d.) at 50 degrees C. Cefradine is separated from its related substances using a mobile phase of acetonitrile-0.02 M sodium 1-octanesulphonate-0.2 M phosphoric acid-water (14.5:10:5:up to 100, v/v/v/v). The flow rate was 1.0 ml min-1 and UV-detection was performed at 254 nm. The method was employed for the quantitative analysis of reference substances, bulk samples and pharmaceutical dosage forms.
Hendrix
C
C
Katholieke Universiteit Leuven, Laboratorium voor Farmaceutische Chemie, Belgium.
Pijcke
M
M
Yun
L M
LM
Roets
E
E
Hoogmartens
J
J
D000097:Acetonitriles; D002515:Cephradine / Q000032:analysis*; D002853:Chromatography, Liquid; D000432:Methanol; D011137:Polystyrenes / Q000737:chemistry*; D012015:Reference Standards; D013056:Spectrophotometry, Ultraviolet
D000097:Acetonitriles; D011137:Polystyrenes; C003771:Amberlite XAD-2 resin; D002515:Cephradine; D000432:Methanol; C032159:acetonitrile