Expression of cell adhesion molecules in jejunum biopsies of children with coeliac disease.
The composition of inflammatory cells as well as the expression of cell adhesion molecules (LFA-1/CD11a, ICAM-1/CD54, CD36) in jejunal biopsies from children with active, untreated coeliac disease (CD) has been analyzed and compared with control biopsies. In CD the number of intraepithelial and lamina propria T cells was greater than in controls. ICAM-1 was found on most cells in the lamina propria; many of them expressed LFA-1 as well. In contrast, no ICAM-1+ and a few LFA-1+ cells were noticed in the epithelium. The brush border from control biopsies reacted with HLADR and anti-CD36. In CD, de novo expression of HLADR was found in the cytoplasm of villous and cryptal enterocytes in contrast, CD36 disappeared from the brush border and no expression of ICAM-1 on the inflamed epithelium was noticed. The results indicate that adhesion molecules other than LFA-1, ICAM-1 and CD36 may be involved in the cellular interactions occurring in the intraepithelial compartment in CD; the lack of ICAM-1 and the reduced expression of LFA-1 on intraepithelial lymphocytes might reflect a defective activation of these cells. Several macrophages were found in the lamina propria in cases of CD; some of them were located beneath the surface epithelium, showed a spindle/dendritic morphology, and expressed the HLADR+, CD36+, CD11a- phenotype. These cells might be stimulated by luminal antigens and might play an important role in subsequent activation of T cells in the lamina propria.
Villanacci
V
V
Istituto di Anatomia Patologica, Spedali Civili, Università di Brescia, Italy.
Facchetti
F
F
Pillan
N
N
Meini
A
A
Donzelli
C
C
Ugazio
A G
AG
D000293:Adolescent; D001706:Biopsy; D002446:Celiac Disease / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D015815:Cell Adhesion Molecules / Q000032:analysis*; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D016130:Immunophenotyping; D007223:Infant; D007413:Intestinal Mucosa / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D007583:Jejunum / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D016131:Lymphocyte Subsets; D008214:Lymphocytes / Q000473:pathology; D008297:Male
D015815:Cell Adhesion Molecules