[The hepatotrophic action of cyclosporin A in a model of hepatic ischemia in the rat].
Liver ischemia is a common surgical situation. In this study the effect of 15 minutes of warm ischemia on liver regeneration in the rat is assessed. From hepatocytic DNA content (cytophotometrically measured), the percentage of regenerating hepatocytes (PRH) and the regenerative gradient (RG) are calculated in normal livers and following different regenerative stimuli: partial hepatectomy (70%) and cyclosporine (20 mg/kg i.p.). In resting livers (both controls and CsA-treated) the PRH and the RG were not modified by ischemia. However, liver regeneration following partial hepatectomy was significantly decreased by ischemia. In the other hand, CsA has increased the PRH and the RG in the four situations analyzed: resting and regenerating liver, both normoperfused or following 15 minutes liver ischemia. It is concluded that the hepatotrophic effect of CsA is not nullified by warm liver ischemia.
Laboratorio de Cirugía Experimental, Universidad del País Vasco.
V
V
Portugal
I
I
García-Alonso
J
J
Bilbao
P
P
Barceló
P
P
Múgica
J
J
Méndez
D000818:Animals; D016572:Cyclosporine / Q000494:pharmacology*; D004247:DNA / Q000032:analysis / Q000187:drug effects; D004195:Disease Models, Animal; D006498:Hepatectomy; D007511:Ischemia / Q000188:drug therapy* / Q000503:physiopathology; D008099:Liver / Q000098:blood supply* / Q000737:chemistry / Q000187:drug effects*; D008115:Liver Regeneration / Q000187:drug effects; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
D016572:Cyclosporine; D004247:DNA
Estudio de la acción hepatotrófica de la ciclosporina A en un modelo de isquemia hepática en la rata.