Serological survey of canine distemper virus infection using enzyme-linked immunosorbent assay.
For detection of antibodies against canine distemper virus (CDV), an enzyme-linked immunosorbent assay (ELISA) was developed using a crude extract from cells infected with the Onderstepoort strain of CDV as antigen. Twenty-six sera from dogs experimentally vaccinated with the Snyder-Hill strain of CDV were compared by ELISA and a standard virus neutralization (NV) test. Since a good correlation between the titers obtained by both tests was observed, ELISA was considered to be a rapid and reliable method for a serological survey of CDV infection. When a total of 167 sera from dogs suspected of canine distemper under natural conditions were examined by the ELISA, 29 of the sera (17%) were found to have low VN antibody titers and high ELISA titers. The reason for the discrepancy in the titers was discussed.
Gemma
T
T
Department of Veterinary Microbiology, Faculty of Agriculture, University of Tokyo, Japan.
Miyashita
N
N
Shin
Y S
YS
Okita
M
M
Mori
T
T
Iwatsuki
K
K
Mikami
T
T
Kai
C
C
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D002522:Chlorocebus aethiops; D004216:Distemper / Q000097:blood / Q000276:immunology* / Q000503:physiopathology; D004217:Distemper Virus, Canine / Q000276:immunology*; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary; D012044:Regression Analysis; D012680:Sensitivity and Specificity; D014709:Vero Cells
D000914:Antibodies, Viral