Effectiveness of sodium 4,5-dihydroxybenzene-1,3-disulfonate (Tiron) in protecting against uranium-induced developmental toxicity in mice.
The effect of Tiron (sodium 4,5-dihydroxybenzene-1,3-disulfonate), a chelating agent used in the treatment of experimental poisoning by a number of heavy metals, on uranium-induced developmental toxicity was evaluated in Swiss mice. A series of four Tiron injections was administered intraperitoneally to pregnant mice immediately after a single subcutaneous injection of 4 mg/kg of uranyl acetate dihydrate given on day 10 of gestation and at 24, 48, and 72 h thereafter. Controls received 0.9% saline with or without uranyl acetate. Tiron effectiveness was assessed at 500, 1000 and 1500 mg/kg per day. Amelioration by Tiron of uranium-induced embryolethality was not noted at the two lower doses. The percentage of dead and resorbed fetuses in the Tiron-treated groups was not statistically different from that in the positive control group. However, treatment at 1500 mg/kg per day showed isolated protective effects against uranium fetotoxicity, such as that evidenced by the lack of differences in fetal body weight between this group and the uranium-untreated group, as well as by a decrease in the number of skeletal defects. According to these results, the ability of Tiron to protect the developing mouse fetus against uranium-induced developmental toxicity offers only modest encouragement with regard to its possible therapeutic potential for pregnant women exposed to this metal.
Laboratory of Toxicology and Biochemistry, School of Medicine, Rovira i Virgili University, Reus, Spain.
M A
MA
Bosque
J L
JL
Domingo
J M
JM
Llobet
J
J
Corbella
D014013:1,2-Dihydroxybenzene-3,5-Disulfonic Acid Disodium Salt / Q000627:therapeutic use*; D000014:Abnormalities, Drug-Induced / Q000209:etiology / Q000517:prevention & control*; D000818:Animals; D005260:Female; D005313:Fetal Death / Q000139:chemically induced / Q000517:prevention & control; D005327:Fetal Resorption / Q000139:chemically induced / Q000517:prevention & control; D007279:Injections, Subcutaneous; D008297:Male; D051379:Mice; D009942:Organometallic Compounds / Q000008:administration & dosage / Q000633:toxicity*; D011247:Pregnancy; D014501:Uranium / Q000633:toxicity*
D009942:Organometallic Compounds; C005460:uranyl acetate; D014501:Uranium; D014013:1,2-Dihydroxybenzene-3,5-Disulfonic Acid Disodium Salt