Influence of different growth factors on a rat choriocarcinoma cell line.
The influence of epidermal growth factor, insulin-like growth factors I and II, insulin, transforming growth factor beta 1 and transferrin on the growth of a postgestational rat choriocarcinoma was examined by the MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] assay. The cell line was cultured in RPMI 1640 medium supplemented with fetal calf serum, beta-mercaptoethanol, glucose, sodium pyruvate and antibiotics. The experiments were done in media supplemented with 10% (optimal) or 3% (suboptimal) fetal calf serum. Among the different growth factors tested, only epidermal growth factor and to a certain extent insulin had a growth-promoting effect by themselves. The other growth factors had either an additive effect in the presence of epidermal growth factor or no effect at all. The cytotrophoblast cells expressed both epidermal growth factor and transferrin receptors whereas the more differentiated giant cells expressed only transferrin receptors.
Rega Institute for Medical Research, University of Leuven, Belgium.
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A
Verstuyf
J
J
Goebels
H
H
Sobis
M
M
Vandeputte
D000818:Animals; D002417:Cattle; D002454:Cell Differentiation / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects; D002822:Choriocarcinoma / Q000378:metabolism* / Q000473:pathology; D003124:Colorimetry / Q000379:methods; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D066246:ErbB Receptors / Q000096:biosynthesis*; D005312:Fetal Blood; D006133:Growth Substances / Q000494:pharmacology*; D007328:Insulin / Q000494:pharmacology*; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology; D007335:Insulin-Like Growth Factor II / Q000494:pharmacology; D051381:Rats; D011990:Receptors, Transferrin / Q000096:biosynthesis*; D013778:Tetrazolium Salts; D013844:Thiazoles; D014168:Transferrin / Q000494:pharmacology; D016212:Transforming Growth Factor beta / Q000494:pharmacology; D014327:Trophoblasts / Q000187:drug effects / Q000378:metabolism; D014407:Tumor Cells, Cultured
D006133:Growth Substances; D007328:Insulin; D011990:Receptors, Transferrin; D013778:Tetrazolium Salts; D013844:Thiazoles; D014168:Transferrin; D016212:Transforming Growth Factor beta; D004815:Epidermal Growth Factor; D007334:Insulin-Like Growth Factor I; D007335:Insulin-Like Growth Factor II; D066246:ErbB Receptors; C022616:thiazolyl blue