Improvement of cytogenetic results in B-cell chronic lymphocytic leukemia using immuno-purified leukemic cells.
Cytogenetics of purified B lymphocytes and whole blood cultures were studied in 14 patients with B-CLL and fluorescence in situ hybridization (FISH) was performed on interphase cells in 12 of these cases in order to detect trisomy 12. Only one minor abnormal clone was found on whole blood cell cultures, clonal anomalies were found in a large proportion of mitoses of 6/14 cultures of purified B cells. FISH detected a trisomy 12 in a case, not found in whole blood cultures but found in 78% metaphases of cultures of purified B lymphocyte. This method may be as sensitive as FISH for the detection of trisomy 12 and improves the detection of clonal abnormalities in CLL.
Laboratoire d'Hématologie, ER CNRS n.21, Centre Hospitalier Universitaire de Poitiers, France.
A
A
Brizard
F
F
Brizard
M
M
Lessard
B
B
Dreyfus
F
F
Guilhot
J
J
Tanzer
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002469:Cell Separation / Q000379:methods*; D002869:Chromosome Aberrations; D005260:Female; D006801:Humans; D007163:Immunosorbent Techniques; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping / Q000379:methods*; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D014314:Trisomy