Visualization of ultraviolet light-induced thymine dimers in DNA by immunoelectron microscopy.
To see the damage of DNA due to ultraviolet-B more distinctly, immunoelectron microscopic studies using a monoclonal antibody against cyclobutane-type thymine dimers were performed. As a result, we could detect the existence of thymine dimers on human genomic DNA and pUC18 plasmid DNA visually. This technique can be useful to locate the photoproducts formed on DNA.
Department of Dermatology, Nagasaki University School of Medicine, Japan.
M U
MU
Udono
M
M
Hori
M
M
Hirayama
H
H
Yoshida
T
T
Suematsu
T
T
Suematsu
T
T
Mori
T
T
Matsunaga
T
T
Mizuno
O
O
Nikaido
D004247:DNA / Q000097:blood / Q000528:radiation effects* / Q000648:ultrastructure; D006801:Humans; D007962:Leukocytes; D016253:Microscopy, Immunoelectron; D010957:Plasmids; D011740:Pyrimidine Dimers / Q000032:analysis*; D014466:Ultraviolet Rays
D011740:Pyrimidine Dimers; D004247:DNA