Type XIV collagen is encoded by alternative transcripts with distinct 5' regions and is a multidomain protein with homologies to von Willebrand's factor, fibronectin, and other matrix proteins.
The combined nucleotide sequences of several overlapping cDNAs provide the first complete amino acid sequence of type XIV collagen. Independent confirmation of the deduced sequence is provided by amino acid sequencing of several tryptic peptides isolated from purified chicken skin type XIV collagen. Comparative analyses show that the amino-terminal non-triple-helical region of alpha 1(XIV) chains contains sequence motifs that are similar to alpha 1(IX) collagen, fibronectin type III repeats, and von Willebrand's factor A-domains. The results also strongly suggest that the alpha 1(XIV) collagen gene is identical to the gene encoding the matrix component previously named undulin. cDNAs covering the 5' region of alpha 1(XIV) mRNA fall into two classes with distinct sequences in their 5'-untranslated regions. We believe the two alternative sequences result from differential splicing of the primary transcript. Interestingly, one of the untranslated sequences shows a high degree of identity with the cis-regulatory translational control sequence in the 5'-untranslated region of a Drosophila ribosomal protein mRNA. We hypothesize therefore that the sequence in alpha 1(XIV) collagen may play a role in the control of alpha 1(XIV) protein synthesis.
Gerecke
D R
DR
Department of Anatomy and Cellular Biology, Harvard Medical School, Boston, Massachusetts 02115.
Foley
J W
JW
Castagnola
P
P
Gennari
M
M
Dublet
B
B
Cancedda
R
R
Linsenmayer
T F
TF
van der Rest
M
M
Olsen
B R
BR
Gordon
M K
MK
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002645:Chickens; D003094:Collagen / Q000235:genetics*; D003238:Connective Tissue / Q000378:metabolism; D004247:DNA / Q000235:genetics; D004330:Drosophila / Q000235:genetics; D005353:Fibronectins / Q000235:genetics*; D015723:Gene Library; D006023:Glycoproteins / Q000235:genetics*; D006801:Humans; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D014176:Protein Biosynthesis; D017433:Protein Structure, Secondary; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D012269:Ribosomal Proteins / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D012867:Skin / Q000378:metabolism; D014158:Transcription, Genetic; D014841:von Willebrand Factor / Q000235:genetics*
C054447:COL14A1 protein, human; D005353:Fibronectins; D006023:Glycoproteins; D046911:Macromolecular Substances; D012333:RNA, Messenger; D012269:Ribosomal Proteins; D014841:von Willebrand Factor; D003094:Collagen; D004247:DNA