Increased expression of endothelin-1 gene in blood vessels of deoxycorticosterone acetate-salt hypertensive rats.
We have recently shown that the content of immunoreactive endothelin-1 is increased in acid extracts from blood vessels of deoxycorticosterone acetate (DOCA)-salt hypertensive rats compared with uninephrectomized control rats. We have also found by immunohistochemistry a significant increase in immunoreactive endothelin-1 in endothelial cells of aorta and mesenteric arteries of DOCA-salt hypertensive rats. In the present study, we investigated preproendothelin-1 gene expression in blood vessels of DOCA-salt hypertensive rats and uninephrectomized control rats. Northern blot analysis using a specific 32P-labeled complementary RNA probe for rat preproendothelin-1 of 319 base pairs revealed a fourfold to fivefold increase in abundance of preproendothelin-1 messenger RNA transcripts in both aorta and mesenteric arteries from DOCA-salt hypertensive rats. Thus, increased immunoreactive endothelin-1 content in blood vessels of DOCA-salt hypertensive rats is secondary to increased preproendothelin-1 gene expression. Exaggerated expression of the preproendothelin-1 gene in mineralocorticoid hypertension may contribute to the maintenance of elevated blood pressure.
Medical Research Council Multidisciplinary Research Group on Hypertension, University of Montreal Canada.
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Larivière
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Day
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Schiffrin
D000818:Animals; D001483:Base Sequence; D001794:Blood Pressure; D001808:Blood Vessels / Q000503:physiopathology; D015152:Blotting, Northern; D003900:Desoxycorticosterone; D019332:Endothelin-1; D016232:Endothelins / Q000235:genetics*; D015870:Gene Expression; D006973:Hypertension / Q000139:chemically induced* / Q000235:genetics* / Q000503:physiopathology; D008297:Male; D015335:Molecular Probes / Q000235:genetics; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011498:Protein Precursors / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012965:Sodium Chloride
D019332:Endothelin-1; D016232:Endothelins; D015335:Molecular Probes; D011498:Protein Precursors; D012333:RNA, Messenger; D003900:Desoxycorticosterone; D012965:Sodium Chloride