SA gene and blood pressure cosegregation using Dahl salt-sensitive rats.
Based on the published cDNA base sequence for the (anonymous) SA gene, a polymerase-chain-reaction (PCR) product from the SA gene was obtained from a Dahl salt-sensitive rat kidney cDNA library. Compared to the published sequence, this product had a 102 base pair insert in the 3' end of the cDNA, probably as the result of alternate mRNA splicing. A StuI restriction fragment length polymorphism detected three alleles among various rat strains using this PCR SA gene probe. Alleles at the SA locus strongly cosegregated with blood pressure (P = .0012) in a salt-fed F2 population derived from crossing Dahl S and Lewis rats. In contrast, a cosegregation analysis of blood pressure and SA alleles in salt-fed F2 rats derived from a Dahl S x Wistar-Kyoto cross gave a negative cosegregation result. It is concluded that in certain genetic backgrounds the SA gene (or a closely linked gene) can contribute a significant component of genetic hypertension.
Department of Medicine, Medical College of Ohio, Toledo 43699.
E L
EL
Harris
H
H
Dene
J P
JP
Rapp
D000818:Animals; D001483:Base Sequence; D001794:Blood Pressure; D015139:Blotting, Southern; D004247:DNA / Q000235:genetics*; D004351:Drug Resistance / Q000235:genetics; D005796:Genes; D016678:Genome; D008297:Male; D015335:Molecular Probes / Q000235:genetics; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D051381:Rats; D011922:Rats, Mutant Strains / Q000235:genetics* / Q000502:physiology; D012965:Sodium Chloride / Q000494:pharmacology*
D015335:Molecular Probes; D012965:Sodium Chloride; D004247:DNA