Proto-oncogene of genomic DNA, related to the human epidermal growth factor receptor (EGFR) gene, from clinically normal domestic animals.
Genomic DNAs of cattle, horses, pigs, dogs, cats and chickens were surveyed using Southern blot hybridization analysis, with a human EGFR cDNA fragment. Several bands with different numbers and molecular weights were observed under the condition of low stringency in the individual animal species. The bands showing DNA polymorphism were observed among bovine genomic PstI-digested DNAs from 4 individuals and EcoRI-digested genomic DNAs from 4 chickens. These results may provide basic data which are useful for analysis of tumorigenetic mechanisms in domestic animals.
Department of Veterinary Pathology, Faculty of Agriculture, Miyazaki University, Japan.
K
K
Kai
S
S
Tateyama
N
N
Miyoshi
R
R
Yamaguchi
K
K
Uchida
M
M
Rostami
D000818:Animals; D000829:Animals, Domestic; D001773:Blood Cells / Q000502:physiology*; D015139:Blotting, Southern; D002415:Cats; D002417:Cattle; D002645:Chickens; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D015342:DNA Probes; D004285:Dogs; D066246:ErbB Receptors / Q000235:genetics*; D005260:Female; D006736:Horses; D006801:Humans; D008297:Male; D010920:Placenta / Q000502:physiology; D011247:Pregnancy; D000090063:Proto-Oncogene Mas; D011519:Proto-Oncogenes; D012016:Reference Values; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D013552:Swine; D013737:Testis / Q000502:physiology*
D015342:DNA Probes; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D004247:DNA; D066246:ErbB Receptors