The neuroanatomic binding pattern of [125I]atrial natriuretic factor in the Japanese quail brain.
Application of quantitative autoradiography technique provided a discrete anatomical distribution pattern of the atrial natriuretic factor (ANF) in the Japanese quail, Coturnix coturnix japonica, brain. The highest binding levels of [125I]ANF were shown to occur in telencephalon areas, such as fasciculus diagonalis Brocae (232 fmol/mg protein), septum (194 fmol/mg protein) and olfactory bulb (153 fmol/mg protein), and in posterior sites, such as nucleus interpeduncularis (177 fmol/mg protein), while lower levels (> 51 < 87 fmol/mg protein) were found in the hypothalamic sites of the diencephalon. The similar ANF receptor density levels in some brain areas of the quail as well as both mammalian and non-mammalian species suggest that this peptide might be involved in osmoregulatory activities (at the brain level) and furthermore indicate a probable functional conservation of ANF in vertebrates.
Tavolaro
R
R
Ecology Department, University of Calabria, Arcavacata di Rende, CS, Italy.
Canonaco
M
M
Franzoni
M F
MF
D000818:Animals; D009320:Atrial Natriuretic Factor / Q000378:metabolism*; D001345:Autoradiography; D001921:Brain / Q000033:anatomy & histology*; D001923:Brain Chemistry / Q000502:physiology*; D003370:Coturnix / Q000378:metabolism*; D007031:Hypothalamus / Q000033:anatomy & histology / Q000378:metabolism; D066298:In Vitro Techniques; D007457:Iodine Radioisotopes; D051381:Rats
D007457:Iodine Radioisotopes; D009320:Atrial Natriuretic Factor