Comparison of nuclear protein SH/DNA ratio in species differing in 2C DNA content and dynamics of DNA endoreplication.
Double fluorescence staining with o-phthalaldehyde (OPT) and propidium diiodide (PI) was applied to measure the nuclear protein SH/DNA ratio in species differing in 2C DNA content. It has been shown that protein SH/DNA ratio decreases along with the increase in 2C DNA content. In endosperm, this ratio is higher than in meristematic nuclei of the same species.
Marciniak
K
K
Department of Plant Cytology and Cytochemistry, University of Lódź, Poland.
D001937:Brassica; D002843:Chromatin / Q000378:metabolism; D004247:DNA / Q000378:metabolism*; D004261:DNA Replication; D005456:Fluorescent Dyes; D009687:Nuclear Proteins / Q000378:metabolism*; D010940:Plant Proteins / Q000378:metabolism*; D010944:Plants / Q000235:genetics / Q000378:metabolism*; D011419:Propidium; D013438:Sulfhydryl Compounds / Q000378:metabolism*; D009764:o-Phthalaldehyde
D002843:Chromatin; D005456:Fluorescent Dyes; D009687:Nuclear Proteins; D010940:Plant Proteins; D013438:Sulfhydryl Compounds; D011419:Propidium; D009764:o-Phthalaldehyde; D004247:DNA