Comparison of nuclear protein SH/DNA ratio in species differing in 2C DNA content and dynamics of DNA endoreplication.
Double fluorescence staining with o-phthalaldehyde (OPT) and propidium diiodide (PI) was applied to measure the nuclear protein SH/DNA ratio in species differing in 2C DNA content. It has been shown that protein SH/DNA ratio decreases along with the increase in 2C DNA content. In endosperm, this ratio is higher than in meristematic nuclei of the same species.
Department of Plant Cytology and Cytochemistry, University of Lódź, Poland.
K
K
Marciniak
D001937:Brassica; D002843:Chromatin / Q000378:metabolism; D004247:DNA / Q000378:metabolism*; D004261:DNA Replication; D005456:Fluorescent Dyes; D009687:Nuclear Proteins / Q000378:metabolism*; D010940:Plant Proteins / Q000378:metabolism*; D010944:Plants / Q000235:genetics / Q000378:metabolism*; D011419:Propidium; D013438:Sulfhydryl Compounds / Q000378:metabolism*; D009764:o-Phthalaldehyde
D002843:Chromatin; D005456:Fluorescent Dyes; D009687:Nuclear Proteins; D010940:Plant Proteins; D013438:Sulfhydryl Compounds; D011419:Propidium; D009764:o-Phthalaldehyde; D004247:DNA