The salvage of deoxycytidine into dCDP-diacylglycerol by macrophages and lymphocytes.
Extracellular deoxycytidine (CdR) was previously shown to be salvaged into water soluble [1] and also into lipidic [2] precursors of phospholipids in stimulated lymphocytes and in lymphoma cells [3]. In this paper we have described that non-dividing murine macrophages salvaged not only 5-3H-CdR but also tritiated thymidine (3H-TdR) mainly into the pools as nucleotides. Chlorpromazine shifted the CdR salvage into a lipidic compound of the cells which was identified as 3H-dCDP-diacylglycerol (dCDP-DAG). After 5-3H-CdR labeling the lipid/DNA ratio was eleven times higher in macrophages than in tonsillar lymphocytes. Thin layer chromatography (TLC) on borate impregnated silica gel plates gave clear separation of CDP-DAG from dCDP-DAG supporting that the extracellular precursor for it is exclusively deoxycytidine and not ribocytidine. No interconversion between deoxy- and and ribocytidine could be observed neither in lymphocytes nor in macrophages.
Department of Biochemistry I., Semmelweis University Medical School, Budapest, Hungary.
A
A
Hrabák
T
T
Spasokukotskaja
A
A
Temesi
M
M
Staub
D000818:Animals; D002648:Child; D002675:Child, Preschool; D002746:Chlorpromazine / Q000494:pharmacology; D003567:Cytidine Diphosphate Diglycerides / Q000378:metabolism*; D004247:DNA / Q000378:metabolism; D003841:Deoxycytidine / Q000378:metabolism*; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D014066:Palatine Tonsil / Q000166:cytology; D010529:Peritoneal Cavity / Q000166:cytology; D011743:Pyrimidines / Q000378:metabolism; D013936:Thymidine / Q000378:metabolism
D003567:Cytidine Diphosphate Diglycerides; D011743:Pyrimidines; D003841:Deoxycytidine; D004247:DNA; D002746:Chlorpromazine; D013936:Thymidine