Enzyme-linked immunosorbent assay for detection of antibodies to the unmodified beta-lactam ring.
Dose- and pH- dependent carbodiimide-mediated coupling of Penicillin-G to polystyrene microtiter-plates that leaves the beta-lactam ring unchanged is described. A new ELISA method was developed using Penicillin-G coated plates. The binding of 3 different monoclonal antibodies as well as human IgG antibodies of the IgG1 and IgG3 subclasses is demonstrated, whereas IgG2, IgG4 and IgE antibodies did not bind. Thus, covalently coupled Penicillin-G can be used to study the immune-response to the unchanged beta-lactam ring in patients receiving penicillin therapy. The new method is complementary to hitherto described techniques, which generally only allow detection of antibodies binding to penicilloyl-groups.
Department of Dermatology, University of Innsbruck, Austria.
G F
GF
Klein
U
U
Stanzl
P O
PO
Fritsch
J M
JM
Varga
D000906:Antibodies / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010400:Penicillin G / Q000737:chemistry / Q000276:immunology*
D000906:Antibodies; D010400:Penicillin G