[Trigonelline in coffee. I. Comparison of thin layer chromatography with high-performance liquid chromatography. Simultaneous determination of caffeine].
Determination of trigonelline in several green and roasted coffees by thin-layer (TLC) and high-performance liquid chromatography (HPLC) were compared. Using TLC, two methods of detection were also compared. In addition, caffeine could be determined using HPLC. The determinations were each made with several variations of extraction and clean-up. Recoveries, variation coefficients and detection limits are listed. The most suitable method was HPLC in connection with hot water extraction. Only water is required as the mobile phase. The values for precision are in most cases better than (trigonelline) or similar to (caffeine) those reported in the literature. The determination of trigonelline by TLC, followed by extraction and photometry, is possible with a similar precision, but the simultaneous determination of caffeine was not tested.
Institut für Lebensmittelchemie, Technischen Universität Braunschweig, Germany.
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Stennert
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Maier
D000470:Alkaloids / Q000032:analysis*; D002110:Caffeine / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D003069:Coffee / Q000737:chemistry*; D015203:Reproducibility of Results
D000470:Alkaloids; D003069:Coffee; D002110:Caffeine; C009560:trigonelline
Trigonellin in Bohnenkaffee. I. Vergleich der Dünnschicht- mit der Hochleistungsflüssigchromatographie. Gleichzeitige Bestimmung von Coffein.