A hyperthermophilic sulfur-reducing archaebacterium, Thermococcus sp. DT1331, isolated from a deep-sea hydrothermal vent.
A hyperthermophilic archaebacterium was isolated from a deep-sea black smoker chimney (depth, 760 m) at the Minami-ensei Knoll (28 degrees 23'N, 127 degrees 38'E). The strain, designated DT1331, was a coccoid shaped bacterium about 0.5 to 1.0 microns in diameter. The cells were surrounded by a cell envelope. The temperature for growth was between 55 degrees C and 93 degrees C with an optimum 80 degrees C. The growth occurred from pH 4.5 to 8.5 and the optimum pH was 6.0. DT1331 required 1% to 5% NaCl for growth and cell lysis was observed below 1% NaCl concentration. The strain was an anaerobic chemoorganotroph requiring elemental sulfur obligately. Organic substrates used included tryptone, peptone, soytone, casein, gelatin, and yeast extract. Under the optimal conditions, DT1331 had a generation time of 50 min and could reach densities of about 1.5 x 10(8) cells/ml. DT1331 was resistant to ampicillin, chloramphenicol, erythromycin, kanamycin, streptomycin, and tetracycline, which was one of the common characteristics of archaebacteria. The G+C content of DT1331 was 52.3 mol%. Analysis of the 16S rRNA gene by restriction enzymes coincided with those of Thermococcus celer, indicating that this strain belonged to the genus Thermococcus.
Kwak
Y S
YS
Korea Ginseng and Tobacco Research Institute, Taejon.
Kobayashi
T
T
Akiba
T
T
Horikoshi
K
K
Kim
Y B
YB
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001105:Archaea / Q000166:cytology / Q000302:isolation & purification / Q000502:physiology*; D001483:Base Sequence; D002455:Cell Division; D003470:Culture Media; D004247:DNA / Q000737:chemistry; D004352:Drug Resistance, Microbial; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D009792:Oceans and Seas; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D015183:Restriction Mapping; D012623:Seawater; D012965:Sodium Chloride / Q000032:analysis; D013696:Temperature
D000900:Anti-Bacterial Agents; D003470:Culture Media; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride; D004247:DNA