Acute ethanol intake produces lipid peroxidation in rat red blood cells membranes.
The effect of acute ethanol intake on lipid peroxidation (LP) and proteins in red blood cells (RBC) was explored. The amount of malondialdehyde (MDA; an indicator of LP) was elevated transiently when the maximal ethanol level in whole blood was observed. In contrast, erythrocyte membrane proteins were not affected. In in vitro experiments both ethanol and acetaldehyde did not alter the MDA levels. These results indicate that the metabolism of ethanol or acetaldehyde beyond the RBCs is required in order to detect LP on these cells.
Departamento de Bioquímica, Facultad de Medicina, Universidad Nacional Autónoma de México, D.F.
J
J
Gutiérrez-Salinas
M
M
Zentella de Piña
E
E
Piña
D000079:Acetaldehyde / Q000633:toxicity; D000818:Animals; D001798:Blood Proteins / Q000187:drug effects; D004591:Electrophoresis, Polyacrylamide Gel; D004910:Erythrocyte Membrane / Q000187:drug effects* / Q000378:metabolism; D000431:Ethanol / Q000008:administration & dosage / Q000633:toxicity*; D066298:In Vitro Techniques; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D008565:Membrane Proteins / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar
D001798:Blood Proteins; D008565:Membrane Proteins; D000431:Ethanol; D008315:Malondialdehyde; D000079:Acetaldehyde