Kasugamycin inhibition of nonsense suppression by thymine-requiring strains of Escherichia coli K12.
Thymine-requiring strains of Escherichia coli suppress nonsense and frame-shift mutations. This appears to occur during translation, suggesting that the lack of activity of an enzyme thymidylate synthase, required for the synthesis of a DNA precursor, alters the fidelity of translation. The aminoglycoside antibiotic kasugamycin, which enhances translational accuracy in vitro, prevents thymine-requiring cells from suppressing. The inhibition of suppression by kasugamycin is not prevented by the introduction of two different kasugamycin-resistance mutations, although the dose required for inhibition increases. These observations support the conclusion that suppression occurs during translation.
Tiganos
E
E
Biology Department, Concordia University, Montréal, Que., Canada.
Herrington
M B
MB
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D009154:Mutation / Q000187:drug effects / Q000235:genetics; D014176:Protein Biosynthesis; D013489:Suppression, Genetic / Q000187:drug effects*; D013941:Thymine / Q000378:metabolism*
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; C100305:kasugamycin; D013941:Thymine