mdr 1 gene-expression and villin synthesis in a colon cancer cell line differentiated by sodium butyrate.
Sodium butyrate (NaBu) but not dimethylsulfoxide (DMSO) induced the synthesis of villin, a protein of the brush border microvilli cytoskeleton, in a rat colon cancer cell line. Neither NaBu nor DMSO altered mdr 1-mRNA expression or multidrug resistance (MDR)--associated cellular transport of doxorubicin. These results show that mdr 1 gene expression and activity are independent of other brush border proteins induced by differentiating agents at the apical pole of the epithelial cell.
Research Group on Digestive Cancers, INSERM 252, Faculty of Medicine, Dijon, France.
J M
JM
Petit
B
B
Chauffert
M T
MT
Dimanche-Boitrel
P
P
Genne
O
O
Duchamp
F
F
Martin
D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D002087:Butyrates / Q000494:pharmacology*; D020148:Butyric Acid; D002352:Carrier Proteins / Q000096:biosynthesis*; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D002462:Cell Membrane / Q000378:metabolism; D003110:Colonic Neoplasms / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D004317:Doxorubicin / Q000493:pharmacokinetics; D004351:Drug Resistance / Q000235:genetics*; D015870:Gene Expression / Q000235:genetics*; D008562:Membrane Glycoproteins / Q000378:metabolism / Q000502:physiology; D008840:Microfilament Proteins / Q000096:biosynthesis*; D051381:Rats; D011919:Rats, Inbred Strains; D014407:Tumor Cells, Cultured / Q000187:drug effects
D002087:Butyrates; D002352:Carrier Proteins; D008562:Membrane Glycoproteins; D008840:Microfilament Proteins; C026615:villin; D020148:Butyric Acid; D004317:Doxorubicin; D004121:Dimethyl Sulfoxide