Immunochemotherapy prevents human colon cancer metastasis after orthotopic onplantation of histologically-intact tumor tissue in nude mice.
A metastatic model of human colon cancer has been previously established using orthotopic onplantation of histologically intact in tissue nude mice. In this study, effects of immunochemotherapy using OK-432, 5-fluorouracil (5-FU) and mitomycin C (MMC) on Col-2-JCK, a human colon cancer xenograft, were evaluated using this model. When 5-FU and MMC were administered without OK-432, liver metastases were not reduced even at maximum tolerated doses of both drugs, although cecal tumor growth was significantly reduced. On the other hand, when combined with OK-432, both 5-FU and MMC reduced liver metastases with synergistic reduction of cecal tumor growth, demonstrating the potential of combining immunotherapy with chemotherapy against metastases.
Department of Surgery, School of Medicine, Keio University, Tokyo, Japan.
T
T
Furukawa
T
T
Kubota
M
M
Watanabe
T H
TH
Kuo
S
S
Kase
Y
Y
Saikawa
H
H
Tanino
T
T
Teramoto
K
K
Ishibiki
M
M
Kitajima
D000818:Animals; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000627:therapeutic use*; D002430:Cecal Neoplasms / Q000188:drug therapy / Q000473:pathology / Q000628:therapy; D003110:Colonic Neoplasms / Q000188:drug therapy / Q000473:pathology* / Q000628:therapy*; D003131:Combined Modality Therapy; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D005472:Fluorouracil / Q000008:administration & dosage; D006801:Humans; D007167:Immunotherapy; D008114:Liver Neoplasms, Experimental / Q000188:drug therapy / Q000517:prevention & control* / Q000556:secondary*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D016685:Mitomycin / Q000008:administration & dosage; D009368:Neoplasm Transplantation; D010844:Picibanil / Q000008:administration & dosage / Q000627:therapeutic use*; D014183:Transplantation, Heterologous
D010844:Picibanil; D016685:Mitomycin; D005472:Fluorouracil