Fibroblast stimulation of breast cancer cell growth in a serum-free system.
Conditioned media from 14 short term fibroblast cell lines were mitogenic for human breast cancer cells with different steroid receptor profiles in serum-free culture. Fibroblast-conditioned medium stimulated tritiated thymidine incorporation in short term culture and growth in a longer proliferation study as measured by the MTT colorimetric assay. Conditioned media from benign and malignant epithelial cells were non-stimulatory for breast cancer cells but that derived from endothelial cells showed similar stimulation to fibroblasts. Partial purification of fibroblast-conditioned medium identified a peptide with a molecular weight of approximately 8 kDa that showed no affinity for heparin and was mitogenic for MCF-7 breast cancer cells.
Department of Surgery, University of Wales College of Medicine, Heath Park, Cardiff, UK.
M C
MC
Ryan
D J
DJ
Orr
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Horgan
D001940:Breast / Q000166:cytology / Q000502:physiology; D001943:Breast Neoplasms / Q000473:pathology*; D002455:Cell Division / Q000187:drug effects / Q000502:physiology; D016895:Culture Media, Serum-Free / Q000494:pharmacology*; D004727:Endothelium / Q000166:cytology / Q000502:physiology; D004815:Epidermal Growth Factor / Q000502:physiology; D004847:Epithelial Cells; D004848:Epithelium / Q000502:physiology; D005260:Female; D005347:Fibroblasts / Q000502:physiology*; D006133:Growth Substances / Q000494:pharmacology; D006801:Humans; D008934:Mitogens / Q000494:pharmacology; D010455:Peptides / Q000494:pharmacology; D013268:Stimulation, Chemical; D014407:Tumor Cells, Cultured / Q000187:drug effects
D016895:Culture Media, Serum-Free; D006133:Growth Substances; D008934:Mitogens; D010455:Peptides; D004815:Epidermal Growth Factor