Rabies virus selectively alters 5-HT1 receptor subtypes in rat brain.
Rabies virus infection in man induces a series of clinical symptoms, some suggesting involvement of the central serotonergic system. The results of the present study show that, 5 days after rabies virus infection in rat, the total reversible high-affinity binding of [3H]5-HT in the hippocampus is not affected, suggesting that 5-HT1A binding is not altered. 5-HT1B sites identified by [125I]cyanopindolol binding are not affected in the cortex 3 and 5 days after the infection. Accordingly, the cellular inhibitory effect of trifluoromethylphenylpiperazine (TFMPP) on the [3H]acetylcholine-evoked release, presumably related to 5-HT1B receptor activity, is not modified 3 days after infection. In contrast, [3H]5-HT binding determined in the presence of drugs masking 5-HT1A, 5-HT1B and 5-HT1C receptors, is markedly (50%) reduced 3 days after the viral infection. These results suggest that 5-HT1D-like receptor subtypes may be affected specifically and at an early stage after rabies viral infection.
Unité Rage, Institut Pasteur, Paris, France.
P E
PE
Ceccaldi
M P
MP
Fillion
A
A
Ermine
H
H
Tsiang
G
G
Fillion
D000109:Acetylcholine / Q000378:metabolism; D000818:Animals; D015152:Blotting, Northern; D001923:Brain Chemistry / Q000502:physiology*; D002540:Cerebral Cortex / Q000378:metabolism; D003342:Corpus Striatum / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism; D066298:In Vitro Techniques; D020120:Iodocyanopindolol; D008297:Male; D009693:Nucleic Acid Hybridization; D010869:Pindolol / Q000031:analogs & derivatives / Q000378:metabolism; D010879:Piperazines / Q000378:metabolism; D011188:Potassium / Q000494:pharmacology; D011818:Rabies / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D011985:Receptors, Serotonin / Q000378:metabolism*; D013569:Synapses / Q000378:metabolism; D013574:Synaptosomes / Q000187:drug effects / Q000378:metabolism
D010879:Piperazines; D011985:Receptors, Serotonin; C018406:1-(3-trifluoromethylphenyl)piperazine; D020120:Iodocyanopindolol; D010869:Pindolol; D000109:Acetylcholine; D011188:Potassium