[Study of genomic polymorphism of Shigella flexneri strains, isolated in various geographic regions].
Strains of Shigella flexneri isolated from patients in 4 geographic regions of the former USSR have been studied by plasmid analysis and fingerprinting techniques. It has been shown that these methods make possible to differentiate phenotypically identical strains for 9 genotypic patterns (7 different plasmid profiles and 3 specific hybridization patterns). Strains of Shigella flexneri isolated from each region possess the similar hybridization patterns independent of serovar and plasmid profiles. Similar hybridization patterns were found among the strains isolated in Erevan and Dushanbe. The strains isolated in Kiev are related to Erevan and Dushanbe strains, but they also possess the specific additional hybridization bands. Tashkent isolates are different from the strains isolated in other geographic regions. They are also of clonal origin in spite of the different serovars and plasmid profiles registered for these strains. Positive correlation has been found between serovar and plasmid profile characteristics of the strains. The clonal origin of the Tashkent isolates is different from the one of Erevan, Dushanbe and Kiev isolates, the latter having a similar clonal origin.
I A
IA
Shaginian
Iu M
IuM
Romanova
V V
VV
Utkin
G E
GE
Rubinov
V M
VM
Bondarenko
A L
AL
Gintsburg
D005798:Genes, Bacterial; D005846:Georgia (Republic); D006801:Humans; D007707:Kyrgyzstan; D009693:Nucleic Acid Hybridization; D011110:Polymorphism, Genetic; D012763:Shigella flexneri / Q000235:genetics* / Q000302:isolation & purification; D013620:Tajikistan; D014455:Ukraine
Izuchenie genomnogo polimorfizma shtammov Shigella flexneri, izolirovannykh v raznykh geograficheskikh regionakh.