Semi-quantitative analysis of cefaclor in human serum by capillary high performance liquid chromatography/fast atom bombardment mass spectrometry.
Capillary high performance liquid chromatography (HPLC) was combined with frit fast atom bombardment (FAB) mass spectrometry (MS) and a detailed procedure has been established for on-line analysis of cefaclor in human serum. The capillary column (0.3 mm i.d.) enabled the introduction of entire effluent to the frit interface for FAB-MS; and a special column switching device for injection and concentration enabled the injection of as large as a 500-microliters volume sample. These conditions gave much higher sensitivity than that of previous HPLC/MS system. Thus, low levels of cefaclor could be successfully identified in sera by its mass spectral measurements 2 h after its single oral administration of a 250-mg capsule in two subjects.
Department of Legal Medicine, Nagoya University School of Medicine, Japan.
K
K
Sato
K
K
Kobayashi
C M
CM
Moore
Y
Y
Mizuno
Y
Y
Katsumata
D000284:Administration, Oral; D000328:Adult; D002433:Cefaclor / Q000008:administration & dosage / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation*; D004867:Equipment Design; D005554:Forensic Medicine / Q000295:instrumentation*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009862:Online Systems; D012016:Reference Values; D012680:Sensitivity and Specificity; D016339:Spectrometry, Mass, Fast Atom Bombardment / Q000295:instrumentation*
D002433:Cefaclor