Rapid and sensitive determination of piroxicam in rat plasma, muscle and skin by high-performance liquid chromatography.
A rapid and precise high-performance liquid chromatographic method for the determination of piroxicam in a variety of biological samples has been developed. A reversed-phase column, isocratic elution and ultraviolet detection were employed. Calibration curves were reproducible and highly linear, with correlation coefficients typically averaging over 0.992. The detection limit of the assay was 100 ng/ml for all biological samples examined (at a signal-to-noise ratio of 3:1). Validation of the method demonstrated a good sensitivity, accuracy and precision. The method has been adopted for a pharmacokinetic study in rats.
Institute of Pharmacology, University of Siena, Italy.
D
D
Cerretani
L
L
Micheli
A I
AI
Fiaschi
G
G
Giorgi
D000818:Animals; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008297:Male; D009132:Muscles / Q000737:chemistry*; D010894:Piroxicam / Q000032:analysis* / Q000097:blood; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D012867:Skin / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
D010894:Piroxicam