Ultraviolet light-induced pathology in the eye: associated changes in ocular aldehyde dehydrogenase and alcohol dehydrogenase activities.
Adult male C57BL/6J inbred mice were subjected to ultraviolet radiation (UVR) exposure (302-nm peak wavelength; average intensity 282 microW/cm2) for 1 h and monitored for ocular aldehyde dehydrogenase (ALDH) and alcohol dehydrogenase (ADH) activity changes over a period of 25 days. Dramatic reductions in activities were observed by 4-6 days postexposure, resulting in enzyme levels of 15-16% of control animals. Major decreases in corneal enzyme levels were predominantly responsible for these changes. Ocular morphology was observed throughout using a photoslit-lamp biomicroscope, with maximum corneal clouding occurring at days 4-6. These data support earlier proposals for major roles for these corneal enzymes in assisting the cornea in protecting the eye against UVR-induced tissue damage.
Downes
J E
JE
Division of Science and Technology, Griffith University, Nathan, Australia.
Swann
P G
PG
Holmes
R S
RS
D000426:Alcohol Dehydrogenase / Q000378:metabolism*; D000444:Aldehyde Dehydrogenase / Q000378:metabolism*; D000818:Animals; D002386:Cataract / Q000473:pathology; D003315:Cornea / Q000201:enzymology / Q000528:radiation effects; D003318:Corneal Opacity / Q000473:pathology; D005123:Eye / Q000201:enzymology* / Q000528:radiation effects*; D007525:Isoelectric Focusing; D007908:Lens, Crystalline / Q000201:enzymology / Q000528:radiation effects; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D012160:Retina / Q000201:enzymology / Q000528:radiation effects; D014466:Ultraviolet Rays / Q000009:adverse effects*
D000426:Alcohol Dehydrogenase; D000444:Aldehyde Dehydrogenase