High level expression of the large subunit of mouse ribonucleotide reductase in a baculovirus system.
The large subunit of ribonucleotide reductase from mouse has been overexpressed in Spodoptera frugiperda cells infected with recombinant baculovirus. The expressed protein was purified by affinity chromatography to apparent homogeneity as determined by SDS-PAGE. The homogeneous protein is recognized in Western blot analysis by a monoclonal antibody raised to the large subunit of ribonucleotide reductase from calf thymus, has the correct N-terminal sequence, and, in the presence of the small subunit of mouse ribonucleotide reductase and nucleoside triphosphate effectors, catalyzes the reduction of both purine and pyrimidine nucleoside diphosphates.
Salem
J S
JS
Department of Medicine, University of Pennsylvania, Philadelphia 19104.
Scott
C P
CP
Li
L S
LS
Cooperman
B S
BS
Rubin
H
H
D000818:Animals; D016367:Baculoviridae; D001483:Base Sequence; D015153:Blotting, Western; D002478:Cells, Cultured; D003001:Cloning, Molecular; D004247:DNA; D051379:Mice; D008969:Molecular Sequence Data; D009036:Moths; D011994:Recombinant Proteins; D012264:Ribonucleotide Reductases / Q000235:genetics* / Q000378:metabolism
D011994:Recombinant Proteins; D004247:DNA; D012264:Ribonucleotide Reductases