Effect of an aromatase inhibitor in human follicular fluid on DNA synthesis of granulosa cells, theca cells, and follicles.
An aromatase inhibitor was identified in human follicular fluid. We sought to examine the effects of this inhibitor on granulosa cells, theca cells, a mixture of granulosa and theca cells, and follicles. Granulosa cells, theca cells, the mixture of granulosa and theca cells, and follicles were incubated with [3H]-thymidine and aromatase inhibitor at 37 degrees C for 40 h in Ham F-10 medium. The radioactivities of acid-soluble fractions were counted. Aromatase inhibitor markedly inhibited the DNA synthesis of granulosa cells and follicles, but it did not inhibit the mixture of granulosa and theca cells. This suggested that aromatase inhibitor was a factor in the follicle paracrine system and acted mainly on granulosa cells.
Department of Obstetrics and Gynecology, Yamagata University School of Medicine, Japan.
T
T
Ono
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Y
Komiya
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SN
Kawagoe
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M
Hiroi
D000818:Animals; D001141:Aromatase / Q000502:physiology; D047072:Aromatase Inhibitors; D004247:DNA / Q000096:biosynthesis*; D005260:Female; D015571:Follicular Fluid / Q000737:chemistry*; D006107:Granulosa Cells / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D006080:Ovarian Follicle / Q000378:metabolism*; D051381:Rats; D013799:Theca Cells / Q000378:metabolism; D013997:Time Factors
D047072:Aromatase Inhibitors; D004247:DNA; D001141:Aromatase