Phosphorylation of NF-I in vitro by cdc2 kinase.
Nuclear factor I (NF-I) was purified to near homogeneity from Ehrlich ascites tumor cells. Mouse NF-I consisted of two peptides with relative molecular masses of 53 and 55 kDa and bound to the authentic NF-I site of the adenovirus DNA with no significant affinity with the CCAAT box. The purified protein was efficiently phosphorylated in a reaction containing immunoprecipitates with an anti-cdc2 kinase antibody. This phosphorylation was abolished when a synthetic peptide containing the consensus phosphorylation site by cdc2 kinase was added in excess to the reaction. These findings indicate that mouse NF-I is phosphorylated in vitro by the cdc2 protein kinase.
Faculty of Pharmaceutical Sciences, Kanazawa University, Ishikawa, Japan.
H
H
Kawamura
K
K
Nagata
Y
Y
Masamune
Y
Y
Nakanishi
D000818:Animals; D001483:Base Sequence; D022762:CCAAT-Enhancer-Binding Proteins; D016203:CDC2 Protein Kinase / Q000378:metabolism*; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D004268:DNA-Binding Proteins / Q000302:isolation & purification / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D007700:Kinetics; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D008970:Molecular Weight; D051839:NFI Transcription Factors; D009687:Nuclear Proteins; D009838:Oligodeoxyribonucleotides; D010766:Phosphorylation; D011401:Promoter Regions, Genetic; D013379:Substrate Specificity; D014157:Transcription Factors / Q000378:metabolism*; D051840:Y-Box-Binding Protein 1
D022762:CCAAT-Enhancer-Binding Proteins; D004268:DNA-Binding Proteins; D051839:NFI Transcription Factors; D009687:Nuclear Proteins; D009838:Oligodeoxyribonucleotides; D014157:Transcription Factors; D051840:Y-Box-Binding Protein 1; C070856:YBX1 protein, human; D016203:CDC2 Protein Kinase