ATP-MgCl2 pre-treatment in an animal model of asphyxial arrest.
ATP-MgCl2 treatment has been found to be a promising intervention in many models of hypoxic/ischemic injury. In this study, the effect of pre-treatment with ATP-MgCl2 on the hemodynamic response to asphyxia in the rat was examined. Rats were anesthetized with halothane and N2O2. A tracheostomy and femoral artery and vein cutdowns were performed. Rats were infused intravenously with either ATP-MgCl2 (approximately 50 mumol/kg) or normal saline (control group) over 15 min. Animals were then asphyxiated for 8 min by occlusion of the ventilator tubing. Following the asphyxia, 1 min of cardiopulmonary resuscitation (CPR) was attempted. Heart rate and blood pressure were monitored continuously throughout the experiment. A total of 41 animals (21 ATP-MgCl2, 20 control) were studied. Analysis of variance (ANOVA) was used to test for differences between groups. The ATP-MgCl2 group had a lower heart rate (HR) and mean arterial pressure (MAP) during the infusion. During asphyxia the ATP-MgCl2 group had a lower MAP but higher HR when compared to the control group. No significant differences were observed in the rates of successful resuscitation between ATP-MgCl2-treated rats (10 of 21, 48%) and controls (12 of 20, 60%). Possible reasons for the apparent lack of benefit of ATP-MgCl2 therapy are discussed.
Department of Pediatrics, Columbus Children's Hospital, OH 43205-2696.
R W
RW
Hickey
R B
RB
Karasic
N G
NG
Bircher
D000255:Adenosine Triphosphate / Q000627:therapeutic use*; D000818:Animals; D001237:Asphyxia / Q000503:physiopathology*; D016887:Cardiopulmonary Resuscitation; D006323:Heart Arrest / Q000503:physiopathology*; D006439:Hemodynamics / Q000187:drug effects*; D011292:Premedication; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
D000255:Adenosine Triphosphate