[Phencyclidine receptors in porcine coronary artery].
By using radioligand assay, there was a phencyclidine (Phe) binding site in porcine coronary artery. This binding was specific, reversible, saturable, and stereoselective. The Scatchard analysis showed that the binding site was a single class, with dissociation constant (Kd) and maximum binding (Bmax) of 27.7 +/- 6.9 nmol.L-1 and 0.82 +/- 0.15 pmol/mg protein, respectively. The displacement experiments revealed that the [3H]Phe binding was displaced by nonradioactive Phe, TCP (Phe receptor agonists), and dextrophan (its antagonist). d-INN, a ligand of sigma receptor, had a weaker activity of displacement. These showed a dose-dependent manner. Both etorphine, an agonist of opioid receptor, and N-methyl-D-aspartic acid (NMDA), an excitatory amino acid, failed to displace the binding. These results suggest that the Phe receptors exist in the porcine coronary artery.
Shi
G G
GG
Department of Neurobiology, Shanghai Medical University, China.
Xu
S F
SF
D000818:Animals; D001158:Arteries / Q000378:metabolism; D001667:Binding, Competitive; D003331:Coronary Vessels / Q000378:metabolism*; D011869:Radioligand Assay; D017478:Receptors, Phencyclidine / Q000378:metabolism*; D013552:Swine
D017478:Receptors, Phencyclidine