[Suppression of replication of swine parvoviral antisense RNA against the NS PPV gene in swine thyroid gland cells].
The possibility of suppression of porcine parvovirus (PPV) reproduction in the culture of thyroid gland cells of a swine that contain the integrated genes for asRNA against the nonstructural proteins of the virus has been studied. 10 cell lines with the asRNA genes have been obtained. The line with the maximal number of integrated gene copies was used to inflict with the parvovirus. The expression of asRNA in this cell line was shown to lead to 95% suppression of PPV replication as compared with the control cell line.
Voskresenskaia
E P
EP
Miroshnichenko
O I
OI
Ponamareva
T I
TI
Savich
O M
OM
Tikhonenko
T I
TI
D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D005822:Genetic Vectors; D010321:Parvoviridae / Q000502:physiology*; D016372:RNA, Antisense / Q000494:pharmacology*; D013552:Swine; D013961:Thyroid Gland / Q000166:cytology / Q000382:microbiology*; D014779:Virus Replication / Q000187:drug effects / Q000235:genetics*
D016372:RNA, Antisense
Podavlenie replikatsii parvovirusa svineĭ antismyslovymi RNK protiv NS gena PPV v kletkakh shchitovidnoĭ zhelezy svin'i.