Ascorbic acid and proliferation of cultured rabbit keratocytes.
The effect of ascorbate and L-ascorbic acid 2-phosphate (P-Asc), a stable derivative of ascorbate, on the growth of cultured rabbit keratocytes was examined. In a 12-day culture, P-Asc (0.05-0.1 mM) and ascorbate (0.05 mM) enhanced the growth of the cells, whereas 0.1-1.0 mM ascorbate was cytotoxic to the cells. An increase in the concentration of P-Asc up to 1.0 mM resulted in no further change in the cell growth. The effect of 0.1 mM P-Asc was observed after the confluency had been achieved in the culture. The lower toxicity of P-Asc may be due to its stability. The presence of both 0.1 mM P-Asc and mouse epidermal growth factor in the medium stimulated cell growth to a higher degree than each factor alone. The results suggested that these two factors enhance the growth of keratocytes in a different manner.
Department of Ophthalmology, Wakayama Medical College, Japan.
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Saika
D000818:Animals; D001205:Ascorbic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology*; D002452:Cell Count; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003238:Connective Tissue / Q000187:drug effects; D003315:Cornea / Q000166:cytology / Q000187:drug effects*; D004247:DNA / Q000096:biosynthesis; D004261:DNA Replication / Q000187:drug effects; D004815:Epidermal Growth Factor / Q000494:pharmacology; D008297:Male; D011817:Rabbits
C011669:ascorbate-2-phosphate; D004815:Epidermal Growth Factor; D004247:DNA; D001205:Ascorbic Acid