A distal region of the CALC-1 gene is necessary for regulated alternative splicing.
The CALC-1 gene exhibits tissue specific alternative splicing with exons 1-4 being spliced to produce the calcitonin mRNA in thyroid C cells and exons 1-3 and 5-6 being joined to produce the CGRP mRNA in neuronal cells. Previous studies have identified an element in intron 3 within the alternatively spliced region which is critical for this effect to occur. We show here that deletion of sequences downstream of the alternatively spliced region also disrupts the tissue specific pattern of alternative splicing. The manner in which these sequences act is discussed.
Division of Molecular Pathology, University College London Medical School, UK.
D A
DA
Horn
D S
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Latchman
D017398:Alternative Splicing; D001483:Base Sequence; D002116:Calcitonin / Q000235:genetics*; D002478:Cells, Cultured; D005091:Exons / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics; D014162:Transfection
D002116:Calcitonin