[Damage to calcium ion-loaded mitochondria by fatty acids and the protective effect of carnitine].
The effect of fatty acids and L-carnitine on Ca2+ retention in rat liver mitochondria have been studied. Ca(2+)-retention was estimated as a sum of consecutive Ca2+ additions which leaded to transient stimulation of respiration coupled with influx of Ca2+ L-carnitine increases the Ca(2+)-retention; such an effect requires ATP. The Ca(2+)-retention was increased in the presence of 50 microM ATP or ADP. In all cases carboxyatractylate prevented the increase in Ca(2+)-retention. Palmitate and FCCP added at concentrations producing similar stimulating effect on respiration inhibit Ca(2+)-retention to about the same degree. The effect of palmitate is strongly diminished by L-carnitine. Again, the L-carnitine effect requires ATP. The data obtained suggest that the protonophoric effect of fatty acid plays a crucial role in Ca(2+)-dependent damage of mitochondria.
Dedukhova
V I
VI
Mokhova
E N
EN
Starkov
A A
AA
Batelli
D
D
Belleĭ
M
M
Bobyleva
V A
VA
D000244:Adenosine Diphosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001278:Atractyloside / Q000031:analogs & derivatives / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D002259:Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone / Q000494:pharmacology; D002331:Carnitine / Q000494:pharmacology*; D005227:Fatty Acids / Q000494:pharmacology*; D008930:Mitochondria, Liver / Q000187:drug effects* / Q000378:metabolism; D051381:Rats
D005227:Fatty Acids; D001278:Atractyloside; D002259:Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D002331:Carnitine; C003853:carboxyatractyloside; D002118:Calcium
Povrezhdenie nagruzhennykh ionami kal'tsiia mitokhondriĭ zhirnymi kislotami i zashchitnoe deĭstvie karnitina.