Activity of membrane, cytosol and lysosome enzymes in organs and blood serum during declamping shock.
The aim of the study was to evaluate the enzyme activity of cellular membranes (GGT), cytosol (ALT, AST) and lysosome (AP, AcP) in the cytosol, whole homogenate and blood serum during declamping shock, following release of abdominal aorta cross-clamping. The aorta was clamped for 60 minutes. An increase in GGT, AP and AcP activities in the cytosol and whole homogenate of the renal cortex, renal medulla, liver, lung, heart and the skeletal muscle occurs after declamping. Rise in the enzymatic activity, especially of acid phosphatase is higher when the aorta above renal arteries was clamped. However, its activity in the blood serum remains unchanged. Alterations in the distribution and the activity of the studied enzymes may indicate that aortic clamping damages the endoplasmic reticulum and lysosomal membranes. Yet, cellular membranes preserve their structural and functional integrity.
Department of Vascular Surgery and Transplantology, Medical Academy of Bialystok.
M
M
Gacko
S
S
Głowiński
A
A
Worowska
J
J
Głowiński
M
M
Zakrzewski
K
K
Worowski
D000135:Acid Phosphatase / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002462:Cell Membrane / Q000201:enzymology*; D003250:Constriction; D003600:Cytosol / Q000201:enzymology*; D004285:Dogs; D007668:Kidney / Q000201:enzymology; D008099:Liver / Q000201:enzymology; D008168:Lung / Q000201:enzymology; D008247:Lysosomes / Q000201:enzymology*; D018482:Muscle, Skeletal / Q000201:enzymology; D009206:Myocardium / Q000201:enzymology; D015427:Reperfusion Injury / Q000201:enzymology*; D012773:Shock, Surgical / Q000201:enzymology*; D000637:Transaminases / Q000378:metabolism; D005723:gamma-Glutamyltransferase / Q000378:metabolism
D005723:gamma-Glutamyltransferase; D000637:Transaminases; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase