Abdominal position of the rat testis is associated with high level of lipid peroxidation.
Experimental cryptorchidism in the adult rat induces lipid peroxidation as a sign of oxidative stress. To further elucidate the role of free radicals and antioxidant enzymes in the degeneration of testis in cryptorchidism, we first studied testes of untreated rats before and after the normal testicular descent. In the second experiment, primary unilateral cryptorchidism was induced by surgically attaching one testis of each rat to the abdomen before testicular descent. The level of lipid peroxidation was detected by formation of fluorescent chromolipids and diene conjugates. At the age of testicular descent (18-21 days), the level of fluorescent chromolipids dropped to one third (p < 0.05). Correspondingly, the level of diene conjugates was 69% (p < 0.05) higher at 18 than at 30 days of age. The antioxidant enzyme activities did not change at the time of testicular descent. Primary unilateral cryptorchidism was induced at the age of 13 days. At 25 or 35 days of age, the level of diene conjugates was higher in the cryptorchid testes than in the contralateral scrotal testes (+39%, p < 0.01, and +51%, p < 0.001, respectively). In the abdominal testes, the mRNA of CuZn superoxide dismutase (SOD) was increased by 69% (p < 0.05) at 25 days, whereas by 35 days of age enzymatic CuZn SOD activity was slightly decreased and catalase activity increased. The present results show that the abdominal position of the testis, either before normal testicular descent or in experimental cryptorchidism, is associated with a high level of lipid peroxidation. The data provide evidence that increased production of reactive oxygen species could contribute to degeneration of the cryptorchid testis. The oxidative testis. The oxidative stress in the cryptorchid testis is not explained by inactivation of antioxidant enzymes.
Department of Physiology, University of Turku, Finland.
V
V
Peltola
I
I
Huhtaniemi
M
M
Ahotupa
D000375:Aging; D000818:Animals; D015152:Blotting, Northern; D002374:Catalase / Q000378:metabolism; D003456:Cryptorchidism / Q000378:metabolism / Q000473:pathology*; D015342:DNA Probes; D015870:Gene Expression; D015227:Lipid Peroxidation; D008297:Male; D008345:Manganese; D009693:Nucleic Acid Hybridization; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism; D013737:Testis / Q000473:pathology*
D015342:DNA Probes; D008345:Manganese; D002374:Catalase; D013482:Superoxide Dismutase