[Pregnancy with testicular spermatozoa].
In a patient with a diagnosis of congenital vas deferens agenesis we decided to carry out a microsurgical epididymal sperm aspiration in order to attempt an in vitro fertilization (IVF). Since during scrotal exploration no epididymus was found (probably as a consequence of a previous surgical biopsy) a testicle biopsy was performed. The latter yielded a few hundred sperms which were injected within the cytoplasm of his wife's oocytes (ICSI technique). Of the 18 oocytes, 3 were harmed during the procedure and 16 hours later signs of normal fertilization were detected in 6 of them, followed by the development of 4 embryos. These were transcervically transferred to the uterus, 3 in 8 cell stage and 1 in 6 cell stage; previously a 20 micron hole had been made with acid Tyrode solution in the pelucida zone of each embryo. Fifteen days later, the result of the HCG beta subunit assay was positive and a month later a single 19 mm normal embryo was detected by ultrasound echography.
Halitus Instituto Médico, Buenos Aires, Argentina.
R
R
Sergio Pasqualini
C
C
Quintans
G R
GR
Valzacchi
N
N
Matarasso
M
M
Martínez
D000328:Adult; D004624:Embryo Transfer; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D006801:Humans; D008297:Male; D008845:Microinjections; D008846:Micromanipulation; D009845:Oligospermia; D011247:Pregnancy; D013084:Sperm-Ovum Interactions; D013094:Spermatozoa; D013737:Testis / Q000166:cytology*
Embarazo con espermatozoides testiculares.