Cloning and nucleotide sequence of the gene encoding dimethyl sulfoxide reductase from Rhodobacter sphaeroides f. sp. denitrificans.
The gene encoding dimethyl sulfoxide (DMSO) reductase, which contains a molybdenum cofactor, of the phototrophic bacterium Rhodobacter sphaeroides f. sp. denitrificans was isolated using an oligonucleotide probe, which was synthesized based on a internal amino acid sequence of the purified enzyme. The DMSO reductase gene coded for 822 amino acids (2466 base pairs, M(r) = 89,206) as a precursor form having a signal peptide of 42 amino acids. The deduced amino acid sequence had high homology with those of some enzymes containing a molybdenum cofactor: trimethyl amine N-oxide reductase (48%), biotin sulfoxide reductase (44%), and DMSO reductase (29%) of Escherichia coli.
Department of Biological Sciences, Faculty of Science, Hiroshima University, Japan.
I
I
Yamamoto
N
N
Wada
T
T
Ujiiye
M
M
Tachibana
M
M
Matsuzaki
H
H
Kajiwara
Y
Y
Watanabe
H
H
Hirano
A
A
Okubo
T
T
Satoh
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D005798:Genes, Bacterial; D007506:Iron-Sulfur Proteins; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000235:genetics*; D012242:Rhodobacter sphaeroides / Q000201:enzymology* / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D015854:Up-Regulation
D004269:DNA, Bacterial; D007506:Iron-Sulfur Proteins; D010088:Oxidoreductases; C045664:dimethyl sulfoxide reductase