Perfadex is superior to Euro-Collins solution regarding 24-hour preservation of vascular function.
BACKGROUND
The aim of this study was to compare Perfadex with Euro-Collins solution regarding 24-hour preservation of endothelium-dependent relaxation and vascular smooth muscle function.


METHODS
The infrarenal aorta of 72 isogenic rats was studied in organ baths as fresh controls, after 24 hours of cold (4 degrees C) storage, and after 24-hour storage followed by transplantation and examination after 7 or 30 days. The thromboxane A2 analogue U-46619 was used to test contractility. Acetylcholine chloride was used to elicit endothelium-dependent relaxation and papaverine hydrochloride, to elicit endothelium-independent relaxation.


RESULTS
With both solutions, all grafts were patent after 7 and 30 days. Vessels preserved in Euro-Collins solution for 24 hours lost 95% (p < 0.001) of their contractility compared with fresh controls; 7 days after transplantation, they had regained 40% of initial contractility, and after 30 days, there was no significant decrease in contractility. Vessels preserved in Perfadex manifested no significant decrease in contractility at any time. Endothelium-dependent relaxation could not be evaluated in vessels stored for 24 hours in Euro-Collins solution because they had lost almost all contractility; 7 days after transplantation, endothelium-dependent relaxation was reduced by 65% (p < 0.001), but at 30 days after transplantation, there was no significant decrease in endothelium-dependent relaxation. Vessels preserved in Perfadex for 24 hours lost 17% (p < 0.05) of endothelium-dependent relaxation, but 7 and 30 days after transplantation, there was no significant decrease in endothelium-dependent relaxation.


CONCLUSIONS
Perfadex, but not Euro-Collins solution, has the capacity to preserve vascular function after 24 hours of storage followed by in vivo reperfusion.
Ingemansson
R
R
Department of Cardiothoracic Surgery, University Hospital, Lund, Sweden.
Massa
G
G
Pandita
R K
RK
Sjöberg
T
T
Steen
S
S
D000818:Animals; D001011:Aorta / Q000502:physiology / Q000637:transplantation; D002951:Citrates / Q000494:pharmacology*; D004353:Drug Evaluation, Preclinical; D004730:Endothelium, Vascular / Q000187:drug effects; D006982:Hypertonic Solutions / Q000494:pharmacology*; D009119:Muscle Contraction / Q000187:drug effects; D009131:Muscle, Smooth, Vascular / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D012996:Solutions / Q000494:pharmacology; D013997:Time Factors; D014021:Tissue Preservation / Q000379:methods*; D014654:Vascular Patency / Q000187:drug effects; D014664:Vasodilation / Q000187:drug effects
D002951:Citrates; C037096:Euro-Collins' solution; D006982:Hypertonic Solutions; C070005:Perfadex; D012996:Solutions