Changes of growth inhibitory factor after stab wounds in rat brain.
The growth inhibitory factor (GIF) is a new metallothionein (MT)-like protein that is downregulated in Alzheimer's disease (AD) brain. The biological function of GIF has not been fully clarified yet. We have raised an antibody to the synthetic polypeptide that is specific for rat GIF. The purified antibody reacted to recombinant GIF and native rat GIF but not to MT or maltose-binding protein. Using the antibody and GIF cDNA probe, we investigated changes of GIF and GIF mRNA by Western and Northern blotting techniques in rat brains after stab wounds. The levels of GIF and GIF mRNA began to increase 4 days postoperation, reached a maximum at 14-21 days and sustained the increased level at least through 28 days. While both glial fibrillary acidic protein (GFAP) and GIF were recognized in astrocytes, the increases of these 2 proteins after stab wounds showed different patterns. The results indicated that GIF could play an important role in the repair after brain damage and also produce new insights into the mechanism of gliosis investigated mainly from the viewpoint of GFAP.
Department of Neurology, Niigata University, Japan.
I
I
Hozumi
T
T
Inuzuka
M
M
Hiraiwa
Y
Y
Uchida
T
T
Anezaki
H
H
Ishiguro
H
H
Kobayashi
Y
Y
Uda
T
T
Miyatake
S
S
Tsuji
D000544:Alzheimer Disease / Q000378:metabolism*; D000595:Amino Acid Sequence; D000704:Analysis of Variance; D000818:Animals; D001483:Base Sequence; D001930:Brain Injuries / Q000378:metabolism*; D004926:Escherichia coli; D005260:Female; D006131:Growth Inhibitors / Q000235:genetics / Q000378:metabolism*; D000090023:Metallothionein 3; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins / Q000096:biosynthesis; D014951:Wounds, Stab / Q000378:metabolism*
D006131:Growth Inhibitors; D000090023:Metallothionein 3; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D011994:Recombinant Proteins