An amino-terminal polypeptide fragment of the influenza virus NS1 protein possesses specific RNA-binding activity and largely helical backbone structure.
The NS1 protein of influenza A virus has the unique property of binding to three apparently different RNAs: poly A; a stem-bulge in U6 small nuclear RNA; and double-stranded RNA. One of our major goals is to determine how the NS1 protein recognizes and binds to its several RNA targets. As the first step for conducting structural studies, we have succeeded in identifying a fragment of the NS1 protein that possesses all the RNA-binding activities of the full-length protein. The RNA-binding fragment consists of the 73 amino-terminal amino acids of the protein. We have developed procedures for obtaining large amounts of the polypeptide in pure form. This has enabled us to establish the RNA-binding properties of this polypeptide and to demonstrate that it retains the ability to dimerize exhibited by the full-length protein. In addition, far-UV CD spectroscopy indicates that this RNA-binding polypeptide is largely (approximately 80%) helical, suggesting that the mode of dimerization of the NS1 protein and of its interaction with RNA is mediated, at least in part, by helices.
Department of Molecular Biology and Biochemistry, Rutgers, State University of New Jersey, Piscataway 08854, USA.
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Qian
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Chien
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Montelione
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D000595:Amino Acid Sequence; D000818:Animals; D002457:Cell Extracts / Q000737:chemistry; D002850:Chromatography, Gel; D002942:Circular Dichroism; D005982:Glutathione Transferase / Q000235:genetics; D009980:Influenza A virus / Q000737:chemistry*; D008156:Luciferases / Q000235:genetics; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D014176:Protein Biosynthesis; D011487:Protein Conformation; D012313:RNA / Q000378:metabolism*; D012330:RNA, Double-Stranded; D012333:RNA, Messenger; D012367:RNA, Viral; D011817:Rabbits; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D012156:Reticulocytes / Q000737:chemistry; D017414:Ribonucleoprotein, U4-U6 Small Nuclear / Q000235:genetics / Q000378:metabolism; D017384:Sequence Deletion; D017361:Viral Nonstructural Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*
D002457:Cell Extracts; C089209:NS1 protein, African horse sickness virus; D010446:Peptide Fragments; D012330:RNA, Double-Stranded; D012333:RNA, Messenger; D012367:RNA, Viral; D011993:Recombinant Fusion Proteins; D017414:Ribonucleoprotein, U4-U6 Small Nuclear; D017361:Viral Nonstructural Proteins; D012313:RNA; D008156:Luciferases; D005982:Glutathione Transferase