Murine IgG subclass antibodies to antigens incorporated in liposomes containing lipid A.
The IgG subclass responses to antigens incorporated in liposomes containing lipid A were investigated using a synthetic malarial antigen (SPf66) and cholera toxin (CT). The antigen-specific IgG subclass response was determined in BALB/c mice immunized with either: (a) SPf66 encapsulated in liposomes containing lipid A, (b) CT bound to the surface of liposomes containing lipid A, or (c) both encapsulated SPf66 and surface-bound CT in the same liposomes. In each case the antibodies to SPf66, CT and lipid A demonstrated an IgG2a predominance. Liposomes containing lipid A not only increased the magnitude of the antibody response to liposomal antigens but elicited predominantly IgG2a subclass antibodies as well.
Glenn
G M
GM
Department of Membrane Biochemistry, Walter Reed Army Institute of Research, Washington, DC 20307-5100, USA.
Rao
M
M
Richards
R L
RL
Matyas
G R
GR
Alving
C R
CR
D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis; D000913:Antibodies, Protozoan / Q000096:biosynthesis; D002772:Cholera Toxin / Q000276:immunology; D003429:Cross Reactions; D007074:Immunoglobulin G / Q000096:biosynthesis* / Q000145:classification*; D008050:Lipid A / Q000276:immunology*; D008081:Liposomes / Q000276:immunology*; D017780:Malaria Vaccines / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D015800:Protozoan Proteins / Q000276:immunology; D011994:Recombinant Proteins
D000907:Antibodies, Bacterial; D000913:Antibodies, Protozoan; D007074:Immunoglobulin G; D008050:Lipid A; D008081:Liposomes; D017780:Malaria Vaccines; D015800:Protozoan Proteins; D011994:Recombinant Proteins; C066269:SPf66 protein, Plasmodium; D002772:Cholera Toxin