Propagation of Mycobacterium lepraemurium on supplemented minimal medium and its experimental pathogenesis.
The splenic tissue of a mouse experimentally infected with M. lepraemurium (Hawaiian strain, M-65) and developing 'rat leprosy', yielded a pure culture of an acid - fast bacterium having all the characteristics of M. lepraemurium on mineral salt minimal medium supplemented with simple sources of C and N, e.g., NH4 -salts, liquid paraffin, urea, gelatin etc. This could be maintained, by serial passages in vitro with good growth. Its indefinite propagation with tissue - free washed, small inoculum on complex media including Ogawa medium was difficult, and its serial sub-culture was practically impossible. The in vitro isolate from supplemented minimal medium could produce pathological lesions in mice typical of rat leprosy.
Department of Medical Microbiology, Calcutta University College of Medicine.
P
P
Banerjee
A N
AN
Chakrabarty
S G
SG
Dastidar
D000818:Animals; D003470:Culture Media; D051379:Mice; D008903:Minerals / Q000494:pharmacology; D009164:Mycobacterium Infections / Q000503:physiopathology*; D009167:Mycobacterium lepraemurium / Q000187:drug effects / Q000254:growth & development* / Q000472:pathogenicity; D051381:Rats
D003470:Culture Media; D008903:Minerals