Enhancement of production of IgM and interferon-beta in human cell lines by poly-lysine.
The promotive effects of poly-cations on immunoglobulin production was investigated using human-human hybridoma cells. Among poly-cations tested, epsilon-poly-L-lysine with hydrochloride (approximately 4 kDa), which has been used as an antibacterial food additive, had the greatest activity in enhancing IgM production of human-human hybridoma HB4C5 cells without stimulating cell proliferation. Immunoglobulin production stimulatory (IPS) activity of epsilon-poly-lysine was not affected by trypsin digestion. It was stable below 60 degrees C but completely inactivated with heating at 100 degrees C for 30 min. epsilon-Poly-lysine also enhanced interferon-beta (IFN-beta) production of human osteosarcoma MG-63 cells, but this stimulatory effect was reduced by the trypsin digestion.
National Research Institute of Vegetables, Shizuoka, Japan.
M M
MM
Yamamoto
Y
Y
Mori
K
K
Osada
H
H
Murakami
D000595:Amino Acid Sequence; D006358:Hot Temperature; D006801:Humans; D006825:Hybridomas; D007075:Immunoglobulin M / Q000096:biosynthesis*; D016899:Interferon-beta / Q000096:biosynthesis*; D008969:Molecular Sequence Data; D011107:Polylysine / Q000031:analogs & derivatives / Q000378:metabolism / Q000494:pharmacology*; D014357:Trypsin / Q000378:metabolism
D007075:Immunoglobulin M; D011107:Polylysine; D016899:Interferon-beta; D014357:Trypsin