Structure of an exocellular polysaccharide of Lactobacillus helveticus TN-4, a spontaneous mutant strain of Lactobacillus helveticus TY1-2.
Lactobacillus helveticus strain TN-4, a spontaneous mutant strain of Lactobacillus helveticus TY1-2, produced an exocellular polysaccharide from reconstituted skim milk. On the basis of the results of methylation analysis, enzymatic digestion, mild Smith degradation, mild acid hydrolysis, acetolysis, and 1D and 2D 1H-NMR spectroscopy, it was concluded that the polysaccharide has a D-galactofuranose containing hexasaccharide repeating unit with the following structure: [formula see text]
Yamamoto
Y
Y
United Graduate School of Agricultural Science, Gifu University, Japan.
Nunome
T
T
Yamauchi
R
R
Kato
K
K
Sone
Y
Y
D000818:Animals; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D006868:Hydrolysis; D007778:Lactobacillus / Q000737:chemistry* / Q000235:genetics; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D008892:Milk / Q000378:metabolism; D008969:Molecular Sequence Data; D015394:Molecular Structure; D011135:Polysaccharides, Bacterial / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification; D001616:beta-Galactosidase / Q000378:metabolism
D011135:Polysaccharides, Bacterial; D001616:beta-Galactosidase