Promoter region of the mouse cyclin-dependent kinase 5-encoding gene.
While cyclin-dependent kinases, such as CDC2 and CDK2, are key regulators of cell-cycle progression, cyclin-dependent kinase 5 (CDK5) is highly expressed in mature neurons with no evident cell-cycle regulation. The 5'-region of the mouse CDK5 gene was isolated and sequenced. The isolated clone included exons 1 through 7. The 5'-flanking region has a high G+C content. There is no TATA box around the transcriptional start points (tsp), as determined by primer extension analysis. One CCAAT box, one AP-1-binding site, two AP-2-binding sites, and one cAMP-responsive element are located upstream from the tsp. Promoter/cat fusion assays showed that the 5.8-kb fragment of this 5'-flanking sequence possessed the promoter activities expressing cat in rat PC12 pheochromocytoma cells. The effect of deletions of the promoter suggested the presence of two negative control elements located from -2.9 kb to -546 bp, and from -212 to -155 upstream from the 5' end of the tsp. Two positive elements from bp -300 to -212 and from -155 to -41 were also detected. In the element from bp -300 to -212, there was a putative NF-IL6-binding sequence. Thus, the CDK5 promoter region contains multiple positive and negative cis-acting regulatory elements, an arrangement which suggests that the regulation of transcription of CDK5 is under complex control.
Department of Biochemistry, Chiba University, School of Medicine, Japan.
T
T
Ishizuka
H
H
Ino
K
K
Sawa
N
N
Suzuki
M
M
Tatibana
D000818:Animals; D001483:Base Sequence; D051360:Cyclin-Dependent Kinase 5; D018844:Cyclin-Dependent Kinases; D005091:Exons; D015971:Gene Expression Regulation, Enzymologic; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D009474:Neurons / Q000201:enzymology; D011401:Promoter Regions, Genetic; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D015183:Restriction Mapping; D014158:Transcription, Genetic
D012333:RNA, Messenger; D051360:Cyclin-Dependent Kinase 5; D017346:Protein Serine-Threonine Kinases; C495906:Cdk5 protein, mouse; D018844:Cyclin-Dependent Kinases