A role for phosphorylation in the proteolytic processing of the human NF-kappa B1 precursor.
A precursor, p105, for one of the subunits (p50) of the NF-kappa B transcription factor, plays an important role in inducible expression of diverse cellular genes. p105 also functions as a cytoplasmic inhibitor for NF-kappa B, and the proteolytic processing of its inhibitory C-terminal region is required for generation of active NF-kappa B. Here, it is reported that the human p105 C-terminal region is phosphorylated in vivo on Ser894 and Ser908, which are potential phosphorylation sites in vitro for proline-directed serine/threonine kinases such as cyclin-dependent kinase. Furthermore, the mutation of these in vivo phosphorylation sites retards p105 processing in vivo, suggesting that p105 processing is regulated in a phosphorylation-dependent manner.
Fujimoto
K
K
Department of Molecular Pathology, Kanazawa University, Ishikawa, Japan.
Yasuda
H
H
Sato
Y
Y
Yamamoto
K
K
D000595:Amino Acid Sequence; D000818:Animals; D017089:Ankyrin Repeat; D001483:Base Sequence; D003546:Cysteine Endopeptidases / Q000378:metabolism; D006801:Humans; D007739:L Cells; D051379:Mice; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000378:metabolism; D016297:Mutagenesis, Site-Directed; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism*; D052002:NF-kappa B p50 Subunit; D010449:Peptide Mapping; D010766:Phosphorylation; D046988:Proteasome Endopeptidase Complex; D011498:Protein Precursors / Q000235:genetics / Q000378:metabolism*; D011499:Protein Processing, Post-Translational / Q000502:physiology*; D012694:Serine / Q000378:metabolism*
D009097:Multienzyme Complexes; D016328:NF-kappa B; D052002:NF-kappa B p50 Subunit; D011498:Protein Precursors; D012694:Serine; D003546:Cysteine Endopeptidases; D046988:Proteasome Endopeptidase Complex