Propagation of Mycobacterium lepraemurium on supplemented minimal medium and its experimental pathogenesis.
The splenic tissue of a mouse experimentally infected with M. lepraemurium (Hawaiian strain, M-65) and developing 'rat leprosy', yielded a pure culture of an acid - fast bacterium having all the characteristics of M. lepraemurium on mineral salt minimal medium supplemented with simple sources of C and N, e.g., NH4 -salts, liquid paraffin, urea, gelatin etc. This could be maintained, by serial passages in vitro with good growth. Its indefinite propagation with tissue - free washed, small inoculum on complex media including Ogawa medium was difficult, and its serial sub-culture was practically impossible. The in vitro isolate from supplemented minimal medium could produce pathological lesions in mice typical of rat leprosy.
Banerjee
P
P
Department of Medical Microbiology, Calcutta University College of Medicine.
Chakrabarty
A N
AN
Dastidar
S G
SG
D000818:Animals; D003470:Culture Media; D051379:Mice; D008903:Minerals / Q000494:pharmacology; D009164:Mycobacterium Infections / Q000503:physiopathology*; D009167:Mycobacterium lepraemurium / Q000187:drug effects / Q000254:growth & development* / Q000472:pathogenicity; D051381:Rats
D003470:Culture Media; D008903:Minerals