Remarks on the vitality of the human cornea after organ culture.
The purpose of this study was to obtain further information on the viability of organ-cultured human cornea. We thus used a specific staining method for succinate dehydrogenase (SDH), which is located in the membrane system of vital mitochondria. We examined fresh and long-term-cultured human corneas. After an initial incubation period in dextran-free culture medium, corneas were stored in a medium containing dextran. With respect to different appearances of the SDH staining, minimal essential medium without dextran seems to have a positive effect on the condition of epithelial cells. After renewal of the medium, keratocytes showed a brief improvement followed by a delayed deterioration, while the endothelial cells were severely damaged. However, best results for all three cell types were observed on the fourth day in a medium containing dextran. We therefore conclude that these corneas were best suited for transplantation.
Department of Opthalmology, Klinikum der RWTH Aachen, Germany.
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Salla
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Redbrake
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Becker
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M
Reim
D000293:Adolescent; D000328:Adult; D000368:Aged; D000375:Aging / Q000502:physiology; D002470:Cell Survival / Q000502:physiology; D002648:Child; D002675:Child, Preschool; D003315:Cornea / Q000166:cytology / Q000201:enzymology / Q000502:physiology*; D003319:Corneal Stroma / Q000166:cytology / Q000502:physiology; D003470:Culture Media; D004728:Endothelium, Corneal / Q000166:cytology / Q000502:physiology; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008875:Middle Aged; D008928:Mitochondria / Q000201:enzymology; D009924:Organ Culture Techniques; D009926:Organ Preservation; D013385:Succinate Dehydrogenase / Q000378:metabolism
D003470:Culture Media; D013385:Succinate Dehydrogenase