Toxicology of 2,4-dichlorophenoxyacetic acid (2,4-D) and its determination in serum and brain tissue using gas chromatography-electron-capture detection.
A gas-liquid chromatographic method with an electron-capture detector was applied for 2,4-dichlorophenoxyacetic acid (2,4-D) determination in the serum and brain tissue of rats acutely intoxicated with the dimethylamine salt of 2,4-D. After extraction with ethyl ether, 2,4-D derivatization was performed using 2-chloroethanol and BCI3. The average recovery values found for serum and brain tissue were 98.5 +/- 4.8 and 93.3 +/- 7.5, respectively. The sensitivity limit of the method was 250 ng/mL for serum and 300 ng/g for brain tissue. The toxic effects of 2,4-D in rats were observed within one-half hour after its oral administration. Results suggest that the toxic mechanism of 2,4-D is related to an action on the central nervous system.
Department of Natural Active Principles, School of Pharmaceutical Sciences UNESP, Araraquara, Brazil.
G H
GH
Oliveira
J
J
Palermo-Neto
D015084:2,4-Dichlorophenoxyacetic Acid / Q000032:analysis* / Q000097:blood / Q000506:poisoning; D000818:Animals; D001923:Brain Chemistry; D002712:Chlorides / Q000737:chemistry / Q000378:metabolism; D002849:Chromatography, Gas / Q000379:methods*; D000431:Ethanol / Q000737:chemistry / Q000378:metabolism; D004986:Ether / Q000737:chemistry; D008297:Male; D051381:Rats; D017208:Rats, Wistar
D002712:Chlorides; D004986:Ether; D015084:2,4-Dichlorophenoxyacetic Acid; D000431:Ethanol