[The effects of dopa and oxygen on RNA concentrations in cultured chick embryonal retinal pigment epithelial cells].
We measured RNA and DNA concentrations in cultured chick embryonal retinal pigment epithelial cells to investigate the effects of dopa and oxygen on DNA and RNA synthesis. RNA/DNA ratios were decreased by addition of 250 microM dopa. Decrease of RNA/DNA ratios was suppressed when the oxygen concentrations were reduced from 20% to 10%. Incubation with medium containing 100 microM dopa increased RNA/DNA ratios in 10% oxygen. Exposure of retinal pigment epithelial cells to 250 microM dopa caused the decrease of RNA concentrations in the retinal pigment epithelial cells, which was ameliorated by lowering oxygen concentrations. However, the addition of 100 microM dopa in 10% oxygen stimulated retinal pigment epithelial cells and seemed to increase RNA concentrations.
Department of Ophthalmology, National Defense Medical College, Saitama-ken, Japan.
T
T
Kanda
K
K
Akeo
A
A
Murakami
Y
Y
Karasawa
S
S
Okisaka
D000818:Animals; D002642:Chick Embryo; D004247:DNA / Q000096:biosynthesis / Q000187:drug effects*; D004295:Dihydroxyphenylalanine / Q000494:pharmacology*; D015259:Dopamine Agents / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D010100:Oxygen / Q000494:pharmacology*; D010857:Pigment Epithelium of Eye / Q000166:cytology / Q000378:metabolism*; D012313:RNA / Q000096:biosynthesis / Q000187:drug effects*
D015259:Dopamine Agents; D004295:Dihydroxyphenylalanine; D012313:RNA; D004247:DNA; D010100:Oxygen