4-hydroxynonenal is degraded to mercapturic acid conjugate in rat kidney.
The cytotoxic lipid peroxidation product 4-hydroxynonenal (HNE) was infused into rat kidney. During the first 2 min a rapid degradation of a 100 microM HNE solution was demonstrated. After 5 min the consumption rate of 4-HNE reached a steady state of about 75 nmoles/ml. The total HNE consumption rate was about 200 nmoles/g w.w./min. The excretion rate into urine was about 0.1% of total HNE consumption. It could be demonstrated that the HNE-mercapturic acid formation takes place in the kidney. The formation of the HNE-mercapturic acid contributes up to 6% to total HNE consumption. Within 10 min of perfusion 2% of the HNE-mercapturic acid were excreted into urine. The residual 98% flow back into the blood circulation.
Clinics of Physical Therapy and Rehabilitation, Medical Faculty (Charité), Humboldt-University Berlin, Germany.
T
T
Petras
W G
WG
Siems
T
T
Grune
D000111:Acetylcysteine / Q000097:blood / Q000378:metabolism*; D000447:Aldehydes / Q000097:blood / Q000378:metabolism*; D000818:Animals; D008658:Inactivation, Metabolic; D007668:Kidney / Q000378:metabolism*; D007700:Kinetics; D015227:Lipid Peroxidation; D008297:Male; D010477:Perfusion; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
D000447:Aldehydes; C027576:4-hydroxy-2-nonenal; D000111:Acetylcysteine