Chromosomal localization of mouse and human genes encoding the splicing factors ASF/SF2 (SFRS1) and SC-35 (SFRS2).
The mammalian SR-type splicing factors ASF/SF2 and SC-35 play crucial roles in pre-mRNA splicing and have been shown to shift splice site choice in vitro. We have mapped the ASF/SF2 gene in mice and humans and the SC-35 gene in mice. Somatic cell hybrid mapping of the human ASF/SF2 gene (SFRS1 locus) reveals that it resides on chromosome 17, and fluorescence in situ hybridization refines this localization to 17q21.3-q22. Recombinant inbred mapping of the mouse ASF/SF2 gene (Sfrs1 locus) and the mouse SC-35 gene (Sfrs2 locus) demonstrates that both genes are located in a part of mouse chromosome 11 that is homologous to human chromosome 17. Mapping of Sfrs1 using F1 hybrid backcross mice between the strains C57BL/6 and DDK places Sfrs1 very near the marker D11Mit38 and indicates that the ASF/SF2 gene is closely linked to the Ovum mutant locus.
Bermingham
J R
JR
University of California at San Diego, La Jolla 92093-0660, USA.
Arden
K C
KC
Naumova
A K
AK
Sapienza
C
C
Viars
C S
CS
Fu
X D
XD
Khotz
J
J
Manley
J L
JL
Rosenfeld
M G
MG
D017398:Alternative Splicing; D000818:Animals; D002874:Chromosome Mapping; D002886:Chromosomes, Human, Pair 17; D006224:Cricetinae / Q000235:genetics; D003433:Crosses, Genetic; D005260:Female; D015186:Hominidae / Q000235:genetics*; D006801:Humans; D006822:Hybrid Cells; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D051379:Mice / Q000235:genetics*; D008815:Mice, Inbred Strains / Q000235:genetics; D009687:Nuclear Proteins / Q000235:genetics*; D012326:RNA Splicing; D016601:RNA-Binding Proteins; D011995:Recombination, Genetic; D012261:Ribonucleoproteins; D000068103:Serine-Arginine Splicing Factors; D013045:Species Specificity
D009687:Nuclear Proteins; D016601:RNA-Binding Proteins; D012261:Ribonucleoproteins; C499372:SRSF2 protein, mouse; C073288:SRSF2 protein, human; D000068103:Serine-Arginine Splicing Factors