[Effect of mao-bushi-saishin-to (MBST), a formula of Chinese medicines, on 48 hr homologous passive cutaneous anaphylaxis in rats].
We studied the effect of oral administration of the extracts from MBST and its component chinese plants, and 1-ephedrine on 48 hr homologous passive cutaneous anaphylaxis (PCA), and histamine- or serotonin-induced skin reaction in rats. Administration (100-1,000 mg/kg) of MBST 1 hr before antigen challenge dose-dependently inhibited PCA. Skin reaction induced by histamine was also inhibited by this formula at 1,000 mg/kg 1 hr prior to the provocation in some degree. The inhibitory component of MBST of PCA was found to be Mao. Further examinations revealed that 1-ephedrine, which is contained in Mao in a large amount, did not substantially inhibit histamine- or serotonin-induced skin reaction. These results indicate that MBST has a significantly inhibitory activity on PCA, to which 1-ephedrine from Mao in the formula almost totally contributes, through the inhibition of chemical mediator release. Since MBST showed some inhibition of histamine-induced skin reaction, on which 1-ephedrine did not affect, it is suggested that some ingredient of MBST responsible for inhibition of PCA may be involved in addition to 1-ephedrine.
Central Research Laboratory, Kotaro Pharmaceutical Co., Ltd.
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Shibata
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Kohno
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Ohata
D000818:Animals; D018926:Anti-Allergic Agents / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004809:Ephedrine / Q000494:pharmacology; D006632:Histamine / Q000494:pharmacology; D008297:Male; D010323:Passive Cutaneous Anaphylaxis / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D012701:Serotonin / Q000494:pharmacology; D013997:Time Factors
D018926:Anti-Allergic Agents; D004365:Drugs, Chinese Herbal; D012701:Serotonin; D006632:Histamine; D004809:Ephedrine