Molecular cloning of the defense factor in the albumen gland of the sea hare Aplysia kurodai.
Aplysianin-A, an antibacterial glycoprotein in the albumen gland of the sea hare Aplysia kurodai, inhibited the growth of both Gram-positive and Gram-negative bacteria. Aplysianin-A cDNA clones were isolated from an albumen gland cDNA library. Sequence analysis reveals that aplysianin-A is produced as a precursor protein of 556 amino acid residues with a signal peptide of 19 amino acid residues and contains 6 potential N-glycosylation sites. Aplysianin-A mRNA was expressed tissue-specifically in the albumen gland. Homology search reveals that aplysianin-A has a 50% overall amino acid sequence homology to achacin, an antibacterial glycoprotein of the giant African snail Achatina fulica.
Department of Biosciences, School of Science, Kitasato University, Kanagawa, Japan.
N
N
Takamatsu
T
T
Shiba
K
K
Muramoto
H
H
Kamiya
D000595:Amino Acid Sequence; D000818:Animals; D001048:Aplysia / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D006023:Glycoproteins / Q000737:chemistry / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000235:genetics; D011506:Proteins / Q000235:genetics* / Q000494:pharmacology; D017421:Sequence Analysis; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D006023:Glycoproteins; D009479:Neuropeptides; D011506:Proteins; C049060:aplysianin A; C077200:achacin