Localization of a corneal basement membrane glycoconjugate in bovine eye.
Lectin histochemistry was used to analyze the ocular surface basement membrane in order to identify novel, tissue-specific glycoconjugates. Soybean agglutinin (SBA), a lectin marker for N-acetylgalactosamine residues, recognized a 130 kDa glycoconjugate in corneal but not conjunctival basement membrane. Following corneal epithelial cell wounding in vitro, the glycoconjugate was not expressed at the epithelial cell-stromal interface until 72-96 h in culture, much later than the expression of other basement membrane molecules. In epithelial cells maintained on various extracellular matrix substrates (laminin, collagen type IV, or a mixture of laminin, collagen type IV and heparan sulfate proteoglycan), the glycoconjugate localization patterns changed with time in culture from a diffuse cellular or perinuclear ring to an extensive extracellular filament network. The presence of SBA in the culture medium did not affect migration or adhesion in either the wound healing model or in culture cells. However, the presence of SBA did delay the deposition of laminin at the zone of basement membrane reformation in the wound healing model. The 130 kDa glycoconjugate is a candidate for molecules which impart a tissue-specificity to the corneal basement membrane, and which may be involved in cell-matrix interaction.
Department of Anatomy and Cell Biology, Wayne State University School of Medicine, Detroit, MI 48201, USA.
M W
MW
Smithson
M A
MA
Kurpakus
D000818:Animals; D001485:Basement Membrane / Q000737:chemistry*; D002417:Cattle; D002478:Cells, Cultured; D003228:Conjunctiva / Q000737:chemistry; D003315:Cornea / Q000737:chemistry* / Q000166:cytology; D004591:Electrophoresis, Polyacrylamide Gel; D004848:Epithelium / Q000737:chemistry; D005123:Eye / Q000737:chemistry; D016650:Fluorescein-5-isothiocyanate; D005456:Fluorescent Dyes; D006001:Glycoconjugates / Q000032:analysis*; D015151:Immunoblotting; D037102:Lectins; D008970:Molecular Weight; D014945:Wound Healing
D005456:Fluorescent Dyes; D006001:Glycoconjugates; D037102:Lectins; D016650:Fluorescein-5-isothiocyanate