Color selection with a hygromycin-resistance-based Escherichia coli-mycobacterial shuttle vector.
Hygromycin-resistance (HyR)-based Escherichia coli-mycobacterial shuttle plasmids have high efficiencies of transformation and a broad mycobacterial host range. We have introduced a lacZ alpha (encoding the alpha-polypeptide fragment of beta-galactosidase (beta Gal))-multiple cloning site cassette into a HyR-based shuttle vector to generate a plasmid with nine unique cloning sites and the added feature of beta Gal color selection in appropriate E. coli host strains.
Howard
N S
NS
Department of Molecular Microbiology and Immunology, School of Hygiene and Public Health, Johns Hopkins University, Baltimore, MD 21205, USA.
Gomez
J E
JE
Ko
C
C
Bishai
W R
WR
D002934:Cinnamates; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000235:genetics*; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D006921:Hygromycin B / Q000031:analogs & derivatives; D009161:Mycobacterium / Q000235:genetics*; D015183:Restriction Mapping
D002934:Cinnamates; D006921:Hygromycin B; C026273:hygromycin A