Ovariectomy decreases plasma triglyceride levels in analbuminaemic rats by lowering hepatic triglyceride secretion.
In mutant analbuminaemic rats (NAR), females demonstrate a more marked hypertriglyceridaemia than males. Ovariectomy decreases triglyceride levels in female NAR. We measured triglyceride secretion rates in vivo as well as the activity of acetyl CoA carboxylase (ACC) and fatty acid synthase (FAS) in hepatic cytosol obtained from female control Sprague-Dawley (SD) rats and NAR with or without ovariectomy. NAR were severely hyperlipidaemic, and triglyceride, cholesterol, apolipoprotein A-I and plasma protein concentrations levels were decreased (all P < 0.01) by ovariectomy. Only triglyceride levels were decreased by ovariectomy in the SD rats (P < 0.05). Oestradiol treatment in ovariectomized NAR restored plasma protein and triglyceride concentrations to levels similar to those observed in intact female NAR and caused a marked increase in plasma cholesterol. Ovariectomy in NAR reduced lipoprotein triglycerides and cholesterol in VLDL, IDL and LDL1, but had little effect on the triglyceride-cholesterol ratio of these particles. Both ACC and FAS activities were markedly increased in NAR vs. SD rats (P < 0.01). This increase was partially corrected by ovariectomy. There was no significant effect of ovariectomy on ACC or FAS activity in the SD rats. Triglyceride secretion rates were significantly increased in NAR vs. SD rats (135 +/- 10 vs. 103 +/- 12 nmol/min per 100 g body weight; P < 0.05). Ovariectomy markedly decreased triglyceride secretion rate in NAR to 69 +/- 6 (P < 0.01), but not in SD rats (92 +/- 8 nmol/min per 100 g body weight, NS). Oestradiol treatment in ovariectomized SD rats restored triglyceride levels but had no significant effect on triglyceride secretion rate (106 +/- 23 nmol/min per 100 g).(ABSTRACT TRUNCATED AT 250 WORDS)
Department of Nephrology and Hypertension, Faculty of Medicine, Utrecht University, The Netherlands.
J A
JA
Joles
C
C
Bijleveld
A
A
van Tol
M J
MJ
Geelen
H A
HA
Koomans
D000103:Acetyl-CoA Carboxylase / Q000378:metabolism; D000818:Animals; D002784:Cholesterol / Q000097:blood; D004958:Estradiol / Q000494:pharmacology; D004967:Estrogens / Q000502:physiology*; D064429:Fatty Acid Synthases / Q000378:metabolism; D005260:Female; D006949:Hyperlipidemias / Q000097:blood / Q000378:metabolism*; D008074:Lipoproteins / Q000097:blood; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D009929:Organ Size; D010052:Ovariectomy; D051381:Rats; D011922:Rats, Mutant Strains; D017207:Rats, Sprague-Dawley; D014280:Triglycerides / Q000097:blood*
D004967:Estrogens; D008074:Lipoproteins; D014280:Triglycerides; D004958:Estradiol; D002784:Cholesterol; D064429:Fatty Acid Synthases; D000103:Acetyl-CoA Carboxylase