Expression of messenger RNA encoding a paraneoplastic cerebellar degeneration-associated antigen in the rat hippocampus.
The PCD17 protein is a paraneoplastic cerebellar degeneration (PCD)-associated neural autoantigen which is recognized by autoantibodies in sera of PCD patients. The localization of the PCD17 mRNA was examined by in situ hybridization histochemistry using a PCD17 cRNA probe. In the cerebellum, the localization of the transcript was in agreement with the distribution of PCD17-immunoreactivity. On the other hand, intense hybridization signals were detected in most of cells in the hippocampus in which notable PCD17-immunoreactivity was undetected. These observations suggest that some negative translational or post-transcriptional mechanisms of the PCD17 mRNA may be involved in neurons of the hippocampus.
Department of Neurology, Kanazawa Medical University, Ishikawa, Japan.
K
K
Sakai
Y
Y
Kitagawa
T
T
Ogasawara
G
G
Hirose
D000818:Animals; D001324:Autoantigens / Q000096:biosynthesis*; D002526:Cerebellar Diseases / Q000378:metabolism* / Q000473:pathology; D004076:Digoxigenin / Q000378:metabolism; D005260:Female; D006624:Hippocampus / Q000378:metabolism*; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D009410:Nerve Degeneration; D009419:Nerve Tissue Proteins / Q000096:biosynthesis*; D010257:Paraneoplastic Syndromes / Q000378:metabolism* / Q000473:pathology; D015347:RNA Probes; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D011917:Rats, Inbred Lew; D011994:Recombinant Proteins / Q000096:biosynthesis
D001324:Autoantigens; C095184:CDR2 protein, human; D009419:Nerve Tissue Proteins; D015347:RNA Probes; D012333:RNA, Messenger; D011994:Recombinant Proteins; D004076:Digoxigenin