Comparative genomic hybridization analysis of human sarcomas: II. Identification of novel amplicons at 6p and 17p in osteosarcomas.
Using comparative genomic hybridization (CGH), we have identified and mapped regions of DNA amplification in primary and metastatic osteosarcomas. Samples were obtained from four patients and ten independent xenografts. Sixty-four percent of the tumors showed increased DNA-sequence copy numbers, affecting 23 different chromosomal sites. Most of these regions were not previously associated with the development and/or progression of these tumors. Amplicons originating from 1q21-q23, 6p, 8q23-qter, and 17p11-p12 were observed most frequently. The 6p and 17p11-p12 amplicons seem to be specific for osteosarcomas, indicating that these regions may harbor genes relevant for the development of these tumors.
Forus
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Department of Human Genetics, University Hospital, Nijmegen, The Netherlands.
Weghuis
D O
DO
Smeets
D
D
Fodstad
O
O
Myklebost
O
O
Geurts van Kessel
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A
D000818:Animals; D002874:Chromosome Mapping; D002886:Chromosomes, Human, Pair 17; D002896:Chromosomes, Human, Pair 6; D005260:Female; D005784:Gene Amplification; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D008819:Mice, Nude; D012516:Osteosarcoma / Q000235:genetics* / Q000556:secondary