Liposome capillary electrophoresis for analysis of interactions between lipid bilayers and solutes.
Liposomes, which mimic biomembranes, were used as a pseudostationary phase in capillary zone electrophoresis. The decrease in the mobility of an analyte owing to the presence of liposomes reflected interaction between the analyte and the liposomes. Equations were derived to calculate the specific capacity factor Ks (the capacity factor, k', normalized to the liposome concentration 1 M) from the migration times and to estimate the difference in free energy, delta(delta G0), of the weak analyte/liposome interactions. The order of Ks values for the drugs tested was aspirin < salicylic acid < warfarin << sulfasalazine. The peptide TyrGlySerThrProGlyCysCys interacted more strongly with the liposomes (Ks = 10.1 M-1) than did TyrGlySerThrProGlySerSer (Ks = 9.1 M-1). These results were similar to those obtained earlier by immobilized liposome chromatography.
Department of Biochemistry, Uppsala University, Sweden.
Y
Y
Zhang
R
R
Zhang
S
S
Hjertén
P
P
Lundahl
D000595:Amino Acid Sequence; D001241:Aspirin / Q000032:analysis / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000379:methods*; D008051:Lipid Bilayers / Q000737:chemistry*; D008081:Liposomes; D008969:Molecular Sequence Data; D015394:Molecular Structure; D010455:Peptides / Q000032:analysis / Q000737:chemistry; D004364:Pharmaceutical Preparations / Q000032:analysis* / Q000737:chemistry*; D012459:Salicylates / Q000032:analysis / Q000737:chemistry; D020156:Salicylic Acid; D012460:Sulfasalazine / Q000032:analysis / Q000737:chemistry; D013816:Thermodynamics; D014859:Warfarin / Q000032:analysis / Q000737:chemistry
D008051:Lipid Bilayers; D008081:Liposomes; D010455:Peptides; D004364:Pharmaceutical Preparations; D012459:Salicylates; D012460:Sulfasalazine; D014859:Warfarin; D020156:Salicylic Acid; D001241:Aspirin