Properties of trichosporin-B-VIa-induced catecholamine secretion from bovine adrenal chromaffin cells.
Trichosporin (TS)-B-VIa, a peptide consisting of 19 amino acid residues and an amino alcohol, causes Ca(2+)-dependent secretion of catecholamines from bovine adrenal chromaffin cells. The TS-B-VIa-induced secretion was greater under alkaline conditions and at a temperature of 37 degrees C compared with that at 21 degrees or 30 degrees C. It was not observed when the peptide was eliminated from the incubation medium. These results strongly suggest that the stimulatory effect of TS-B-VIa on the secretion is reversible and dependent on the temperature and pH of the incubation medium.
Department of Pharmacology, School of Medicine, Iwate Medical University, Morioka, Japan.
E
E
Tachikawa
S
S
Takahashi
K
K
Mizuma
T
T
Kashimoto
Y
Y
Nagaoka
A
A
Iida
T
T
Fujita
D000311:Adrenal Glands / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D023181:Antimicrobial Cationic Peptides; D002118:Calcium / Q000378:metabolism; D002395:Catecholamines / Q000378:metabolism*; D002417:Cattle; D002478:Cells, Cultured; D007473:Ion Channels / Q000187:drug effects / Q000378:metabolism; D008969:Molecular Sequence Data; D010455:Peptides; D013696:Temperature
D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides; D002395:Catecholamines; D007473:Ion Channels; D010455:Peptides; C089061:trichosporin B-VIa; D002118:Calcium