Differentially transcribed regions of Haloferax volcanii genome depending on the medium salinity.
To identify genomic regions involved in osmoregulation in the extremely halophilic archaeon Haloferax volcanii, we used a technique which involves hybridization of cDNAs obtained at different salinities against a cosmid library of the organism. Both low and high salt concentrations trigger differential expression; however, adaptation to low salinities seems to elicit a wider response. The presence of a large domain within the largest of the megaplasmids with a strong response to low salt concentrations is noteworthy.
Ferrer
C
C
Departamento de Genética y Microbiología, Universidad de Alicante, Spain.
Mojica
F J
FJ
Juez
G
G
Rodríguez-Valera
F
F
D015152:Blotting, Northern; D015139:Blotting, Southern; D002874:Chromosome Mapping; D003470:Culture Media; D018076:DNA, Complementary; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects*; D016680:Genome, Bacterial; D006216:Halobacteriaceae / Q000235:genetics*; D012965:Sodium Chloride / Q000494:pharmacology*; D014158:Transcription, Genetic / Q000187:drug effects*; D014882:Water-Electrolyte Balance
D003470:Culture Media; D018076:DNA, Complementary; D012965:Sodium Chloride