[Monoclonal antibody mNI-58 inducing morphological changes in a human monocyte-like cell line, U937, stimulated with phorbol myristate acetate (PMA)].
A mouse monoclonal antibody (mAb), designated mNI-58, was produced. The mNI-58 markedly induced spread formation of the phorbol myristate acetate (PMA)-stimulated U937 cells (referred to as PMA-U937 cells) but not that of the resting U937 cells. This spread formation was completely blocked in the presence of cytochalasin D or cycloheximide. It was partially blocked by an mAb against CD18 (lymphocyte function-associated antigen-1 beta). These results suggest that mNI-58 is capable of transducing a signal for spread formation of the PMA-U937 cells.
Department of Infections, Kitasato University School of Nursing, Kanagawa, Japan.
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Ikewaki
D000818:Animals; D000911:Antibodies, Monoclonal; D018968:CD58 Antigens / Q000276:immunology; D003513:Cycloheximide / Q000494:pharmacology; D015638:Cytochalasin D / Q000494:pharmacology; D006801:Humans; D051379:Mice; D009000:Monocytes / Q000166:cytology*; D015398:Signal Transduction; D013755:Tetradecanoylphorbol Acetate / Q000037:antagonists & inhibitors / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D018968:CD58 Antigens; D015638:Cytochalasin D; D003513:Cycloheximide; D013755:Tetradecanoylphorbol Acetate