Morphological transformation induced by glass fibers in BALB/c-3T3 cells.
Studies were conducted to determine whether 1) glass fibers can induce morphological transformation in BALB/c-3T3 cells, 2) the transforming activity of glass fibers is related to fiber size, and 3) transformed cells induced by glass fibers possess neoplastic properties. In the transformation assay, BALB/c-3T3 cells were treated with three different types of glass fibers: Manville code 100 (JM-100, Manville Corp., Denver, CO), Owens-Corning AAA-10 (AAA-10, Owens-Corning Corp., Toledo, OH), and Owens-Corning general building insulation (ISL, Owens-Corning Corp.) fibers. The neoplastic properties were investigated using the soft agar cloning and gene transfection methods. All three different glass fibers were cytotoxic at high concentrations and induced dose-related increases in morphological transformation. The transforming activity was inversely related to fiber size, with AAA-10 showing higher activity than JM-100 and JM-100 showing higher activity than ISL fiber. Transformed cells induced by glass fibers exerted anchorage-independent growth (90%) and DNA transfection-mediated transformation (100%). These results indicate that glass fibers are capable of transforming mammalian (BALB/c-3T3) cells in vitro as a function of their physical properties and that glass fiber-induced transformed cells possess preneoplastic characteristics.
Division of Respiratory Disease Studies, National Institute for Occupational Safety and Health, Morgantown, West Virginia 26505-2888, USA.
H G
HG
Gao
W Z
WZ
Whong
W G
WG
Jones
W E
WE
Wallace
T
T
Ong
D016475:3T3 Cells; D000818:Animals; D002273:Carcinogens; D002448:Cell Adhesion; D002471:Cell Transformation, Neoplastic; D004305:Dose-Response Relationship, Drug; D005898:Glass; D051379:Mice; D008807:Mice, Inbred BALB C; D014162:Transfection
D002273:Carcinogens