Persistence of increased levels of ribosomal gene activity in CHO-K1 cells treated in vitro with demethylating agents.
The rate of ribosomal gene activity was evaluated by silver staining of the Nucleolus Organisers (NOs) in cultured CHO-K1 cells after a 12 h pulse with two demethylating agents (L-ethionine and 5-azacytidine). Silver staining of the NOs was measured every 24 h, from 24 up to 110 h after seeding. The purpose was to test the hypothesis that drug-induced demethylation is associated to heritable modifications of rDNA activity. Ribosomal gene activity was shown to be significantly increased by both agents. The increase persisted throughout the experiments, thereby suggesting the heritability of this epigenetic modification. The analysis of heritable DNA damage or modification is an important task in studying the risk of cancer onset and the mechanisms of cancer induction. In these studies two main results were obtained: (i) heritable DNA variations can be induced by both mutational and epigenetic changes; (ii) the modified end-point was not negatively selected.
Dipartimento di Genetica e Biologia Molecolare, Università La Sapienza, Rome, Italy.
P
P
Giancotti
C
C
Grappelli
I
I
Poggesi
M
M
Abatecola
A
A
de Capoa
R
R
Cozzi
P
P
Perticone
D000818:Animals; D000963:Antimetabolites / Q000633:toxicity*; D001374:Azacitidine / Q000378:metabolism / Q000633:toxicity; D016466:CHO Cells; D002872:Chromosome Deletion; D002999:Clone Cells; D006224:Cricetinae; D004275:DNA, Ribosomal / Q000187:drug effects* / Q000378:metabolism; D005001:Ethionine / Q000378:metabolism / Q000633:toxicity; D015972:Gene Expression Regulation, Neoplastic; D018095:Germ-Line Mutation; D008745:Methylation / Q000187:drug effects; D008940:Mitotic Index / Q000187:drug effects; D009153:Mutagens / Q000378:metabolism / Q000633:toxicity*; D009697:Nucleolus Organizer Region / Q000187:drug effects / Q000378:metabolism; D016622:Silver Staining
D000963:Antimetabolites; D004275:DNA, Ribosomal; D009153:Mutagens; D001374:Azacitidine; D005001:Ethionine