Sequence and expression of a xylanase gene from the hyperthermophile Thermotoga sp. strain FjSS3-B.1 and characterization of the recombinant enzyme and its activity on kraft pulp.
A gene expressing xylanase activity was isolated from a genomic library of Thermotoga sp. strain FjSS3-B.1. The sequence of the gene shows that it encodes a single domain, family 10 xylanase. The recombinant enzyme has extremely high thermal stability, activity over a relatively broad pH range, and activity on Pinus radiata kraft pulp.
Saul
D J
DJ
Centre for Gene Technology, University of Auckland, New Zealand.
Williams
L C
LC
Reeves
R A
RA
Gibbs
M D
MD
Bergquist
P L
PL
D000595:Amino Acid Sequence; D001483:Base Sequence; D001709:Biotechnology; D004269:DNA, Bacterial / Q000235:genetics; D004795:Enzyme Stability; D015870:Gene Expression; D005798:Genes, Bacterial; D006089:Gram-Negative Anaerobic Bacteria / Q000201:enzymology* / Q000235:genetics*; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D010209:Paper; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D013696:Temperature; D043325:Xylan Endo-1,3-beta-Xylosidase; D014995:Xylosidases / Q000235:genetics* / Q000378:metabolism*
D004269:DNA, Bacterial; D011994:Recombinant Proteins; D014995:Xylosidases; D043325:Xylan Endo-1,3-beta-Xylosidase