A keratan sulfate proteoglycan marks the boundaries in the cortical barrel fields of the adult rat.
The present study uses the monoclonal antibody TED 15 to examine the distribution of a keratan sulfate proteoglycan, ABAKAN, in the barrel field of the rat somatosensory cortex. At birth there is very little ABAKAN present in the somatosensory cortex. The levels of this proteoglycan increase during development until the highest levels are reached in the adult rat. Immunohistochemistry reveals that the TED 15 immunoreaction product marks the boundaries between cortical barrels. At postnatal day 7 (P7) the proteoglycan is localized specifically to the boundary regions. As the brain matures, the levels of ABAKAN increase in the barrel hollows and in the surrounding cortex; however the boundary regions maintain a higher level of expression even in the adult animal. The high levels of ABAKAN observed in the adult barrel fields indicate that unlike other boundary molecules this proteoglycan may be involved in maintaining the structure of the adult somatosensory cortex.
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Department of Anatomy and Neurobiology, University of Tennessee, Memphis 38163, USA.
Geisert
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D000818:Animals; D000831:Animals, Newborn; D011508:Chondroitin Sulfate Proteoglycans / Q000378:metabolism*; D015151:Immunoblotting; D007150:Immunohistochemistry; D007632:Keratan Sulfate / Q000378:metabolism*; D000071234:Lumican; D009416:Nerve Regeneration / Q000502:physiology; D009475:Neurons, Afferent / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013003:Somatosensory Cortex / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*
D011508:Chondroitin Sulfate Proteoglycans; C000604308:Lum protein, rat; D000071234:Lumican; D007632:Keratan Sulfate