PCR-ribotyping of type isolates of currently accepted Exophiala and Phaeococcomyces species.
Portion of the ribosomal repeat of the type strains of the genera Exophiala and Phaeococcomyces were subjected to RFLP analysis. The amplicon length of the small subunit rRNA, the fragment NS1-NS24, was found to vary between 1800 to 3200 nucleotides. In contrast, the length of the fragment ITS1-ITS4 comprising the internal transcribed spacers (ITS1 and ITS2) was found to be constant at 600 nucleotides. Analysis of restriction profiles confirmed the synonymy of Exophiala dermatitidis and Mycotorula schawaii. Torula bergeri and Sporotrichum gougerotii were found to be identical to Phaeoannellomyces elegans, but different from their alleged synonym E. castellanii. A phenogram is presented.
Centraalbureau voor Schimmelcultures, Baarn, The Netherlands.
J M
JM
Uijthof
G S
GS
de Hoog
D004275:DNA, Ribosomal / Q000235:genetics; D005093:Exophiala / Q000145:classification / Q000235:genetics*; D003904:Mitosporic Fungi / Q000145:classification / Q000235:genetics*; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D012331:RNA, Fungal / Q000235:genetics*; D012335:RNA, Ribosomal / Q000235:genetics*; D017422:Sequence Analysis, DNA
D004275:DNA, Ribosomal; D012331:RNA, Fungal; D012335:RNA, Ribosomal