Delayed glucocorticoid-dependent development of the insulin response after heat shock in cultured fetal hepatocytes: correlation with a transient defect in glucocorticoid receptor binding property.
The effects of a mild heat shock were investigated on glucocorticoid-induced maturation of the insulin glycogenic response and on glucocorticoid receptor (GR) in cultured 15-day-old fetal rat hepatocytes. In all experiments, cell heating at 42.5 degrees C from 30 to 90 min was applied at Day 1 of culture before exposure to glucocorticoids. In never heated cells, the insulin stimulation of glycogenesis, measured bh [14C]glucose incorporation into glycogen for 3 h, developed to be maximal after 32 h in the presence of 100 nM dexamethasone (2.4-fold). In cells preheated at 42.5 degrees C for times equal to or greater than 60 min before being returned to 37 degrees C in dexamethasone-containing medium, the insulin response was not seen after 32 h (1.3-fold versus 2.4-fold) but was clearly expressed after 48 h. Heat treatment induced a progressive decrease in intact cell GR binding, reaching 40% after 1 h. When cells were returned to 37 degrees C, GR binding following a lag time of 8 h increased up to complete restoration after 24 h. Such heat shock-induced variations affected the number of GR binding sites with little change in GR binding affinity, while no modifications were seen in the 97-kDa GR level from whole cell extracts as revealed by Western immunoblotting using an anti-GR antibody (BuGR2). [35S]Methionine metabolic labeling showed a reversible heat-stimulated synthesis of 70- and 90-kDa heat shock proteins (Hsps). Variations in Hsp90 level revealed by Western immunoblotting using an anti-Hsp90 antibody (AC88) were inversely correlated with time with GR binding. Therefore, a mild heat shock applied to cultured fetal hepatocytes led to a delayed development of the glycogenic response to insulin linked to a defect in GR binding property with no alteration in the GR protein level.
Laboratoire de Biologie-Odontologie, Université Paris 7, France.
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D000818:Animals; D002478:Cells, Cultured; D003907:Dexamethasone / Q000494:pharmacology*; D005938:Glucocorticoids / Q000494:pharmacology*; D018841:HSP90 Heat-Shock Proteins / Q000096:biosynthesis; D006360:Heat-Shock Proteins / Q000096:biosynthesis; D018869:Heat-Shock Response / Q000502:physiology*; D007328:Insulin / Q000494:pharmacology*; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000196:embryology / Q000378:metabolism*; D008112:Liver Glycogen / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D011965:Receptors, Glucocorticoid / Q000378:metabolism*
D005938:Glucocorticoids; D018841:HSP90 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D007328:Insulin; D008112:Liver Glycogen; D011965:Receptors, Glucocorticoid; D003907:Dexamethasone