S-nitrosoglutathione induces formation of nitrosylmyoglobin in isolated hearts during cardioplegic ischemia--an electron spin resonance study.
Previously, it has been shown that *NO donor S-nitrosoglutathione (GSNO) improves the postischemic functional recovery in crystalloid buffer-perfused isolated rat hearts subjected to cardioplegic ischemia. Supplementation of cardioplegic solution with nitronyl nitroxide, a scavenger of *NO, antagonized this protective effect. Using low temperature ESR, we have detected nitrosylmyoglobin (MbNO) in rat hearts subjected to cardioplegic ischemia in the presence of GSNO (20-200 mumol/l). During aerobic reperfusion MbNO signal intensity gradually decreased, but persisted for up to 30 min of aerobic reperfusion. We conclude that MbNO is an endogenous marker of *NO release in myocardial tissues. Implications of MbNO formation are discussed with respect to cardioprotection during ischemia- and reperfusion-induced myocardial injury.
Konorev
E A
EA
Biophysics Research Institute, Medical College of Wisconsin, Milwaukee 53226-0509, USA.
Joseph
J
J
Kalyanaraman
B
B
D000818:Animals; D002314:Cardioplegic Solutions; D004578:Electron Spin Resonance Spectroscopy; D016166:Free Radical Scavengers; D005978:Glutathione / Q000031:analogs & derivatives* / Q000494:pharmacology; D008297:Male; D017202:Myocardial Ischemia / Q000139:chemically induced / Q000378:metabolism*; D015425:Myocardial Reperfusion; D009206:Myocardium / Q000378:metabolism*; D009211:Myoglobin / Q000031:analogs & derivatives* / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D009603:Nitroso Compounds / Q000494:pharmacology*; D010477:Perfusion; D051381:Rats; D017207:Rats, Sprague-Dawley; D026422:S-Nitrosoglutathione
D002314:Cardioplegic Solutions; D016166:Free Radical Scavengers; D009211:Myoglobin; D009603:Nitroso Compounds; C031560:myoglobin nitroxide; D009569:Nitric Oxide; D026422:S-Nitrosoglutathione; D005978:Glutathione