Biodistribution and radioimmunopharmacokinetics of 131I-Ama monoclonal antibody in atherosclerotic rabbits.
Monoclonal antibodies have been raised against Ama isolated from human and experimental atherosclerotic plaque. 131I-Ama-MoAb in the whole antibody form was injected into normal NZW rabbits and Watanabe hyperlipidemic rabbits. Biodistribution studies showed that atheromatous aortas had a significantly higher (5-7X) uptake of 131I-Ama-MoAb than that of normal aortas. However, 131I-Ama-MoAb was cleared very slowly from atherosclerotic rabbits. As a result, atheromas could not be identified by imaging because of the low target to non-target ratios.
Chakrabarti
M
M
Department of Hospital Pharmacy, Medical University of South Carolina, Charleston 29425, USA.
Cheng
K T
KT
Spicer
K M
KM
Kirsch
W M
WM
Fowler
S D
SD
Kelln
W
W
Griende
S
S
Nehlsen-Cannarella
S
S
Willerson
R
R
Spicer
S S
SS
D000818:Animals; D000911:Antibodies, Monoclonal / Q000493:pharmacokinetics*; D001161:Arteriosclerosis / Q000000981:diagnostic imaging / Q000378:metabolism*; D006801:Humans; D007457:Iodine Radioisotopes / Q000493:pharmacokinetics*; D008314:Malonates / Q000276:immunology*; D008657:Metabolic Clearance Rate; D011817:Rabbits; D011877:Radionuclide Imaging; D014018:Tissue Distribution
D000911:Antibodies, Monoclonal; D007457:Iodine Radioisotopes; D008314:Malonates; C019249:aminomalonic acid