A comparison of the leakage of a monoclonal antibody from various immunoaffinity chromatography matrices.
Antibody leakage from immunoaffinity chromatography (IAC) matrices could reduce the working life of the IAC matrix and/or contaminate parenteral products, purified by IAC. There is therefore a need to measure the leakage of antibody from IAC matrices and to reduce such leakage. Using sensitive ELISAs it was found that the type of activated matrix, the buffer, the presence of proteases in the feedstock and the storage of IAC matrices between runs could all effect antibody leakage.
Beer
D J
DJ
Production Division, Centre for Applied Microbiology and Research, Porton Down, Salisbury, Wiltshire, UK.
Yates
A M
AM
Randles
S C
SC
Jack
G W
GW
D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification*; D001710:Biotin; D002846:Chromatography, Affinity / Q000379:methods*; D003488:Cyanogen Bromide; D004797:Enzyme-Linked Immunosorbent Assay; D005069:Evaluation Studies as Topic; D005640:Follicle Stimulating Hormone / Q000276:immunology / Q000302:isolation & purification; D037201:Follicle Stimulating Hormone, beta Subunit; D006801:Humans; D007093:Imidazoles; D007163:Immunosorbent Techniques; D051379:Mice; D051381:Rats; D012685:Sepharose / Q000031:analogs & derivatives*; D013972:Thyrotropin / Q000276:immunology / Q000302:isolation & purification
D000911:Antibodies, Monoclonal; D037201:Follicle Stimulating Hormone, beta Subunit; D007093:Imidazoles; C032392:Affi-Gel 10; C006900:N,N-carbonyldiimidazole; D001710:Biotin; D005640:Follicle Stimulating Hormone; D013972:Thyrotropin; D012685:Sepharose; D003488:Cyanogen Bromide