Plasma protein leakage and local secretion of proteins assessed in sputum in asthma and COPD. The effect of inhaled corticosteroids.
Asthma and chronic obstructive pulmonary disease (COPD) are characterized by chronic airway inflammation with cell infiltration, increased plasma exudation and abnormal local secretion of proteins. We have analysed whether sputum differs in this respect between asthma (n = 9) and COPD (n = 9), and whether inflammatory markers in sputum are affected by treatment. In non-smoking asthma patients there was more plasma protein leakage, based on the relative coefficient of excretion Q alpha 2macroglobulin/QIgG (P = 0.03). There was less local secretion of sIgA and lactoferrin than in COPD (P < 0.05). Tryptase was slightly higher in sputum from asthma than from COPD (P < 0.05), whereas eosinophil cationic protein and myeloperoxidase were similar. After treatment with glucocorticosteroids, there was a reduction in the Q alpha 2macroglobulin/Qalbumin (P < 0.015), but no effect was seen on the levels of products from local cells. We conclude that sputum analysis is useful to study the local inflammatory process in asthma and COPD.
Department of Pulmonology, University of Amsterdam, The Netherlands.
D F
DF
Schoonbrood
T A
TA
Out
R
R
Lutter
C M
CM
Reimert
F J
FJ
van Overveld
H M
HM
Jansen
D000280:Administration, Inhalation; D000305:Adrenal Cortex Hormones / Q000008:administration & dosage / Q000494:pharmacology*; D000328:Adult; D000368:Aged; D001249:Asthma / Q000188:drug therapy / Q000201:enzymology / Q000378:metabolism*; D015415:Biomarkers; D001798:Blood Proteins / Q000378:metabolism*; D053818:Chymases; D004804:Eosinophils / Q000737:chemistry; D005260:Female; D005541:Forced Expiratory Volume; D006801:Humans; D008173:Lung Diseases, Obstructive / Q000188:drug therapy / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000201:enzymology; D009195:Peroxidase / Q000032:analysis; D012129:Respiratory Function Tests; D012697:Serine Endopeptidases / Q000032:analysis; D012907:Smoking / Q000378:metabolism; D013183:Sputum / Q000737:chemistry* / Q000166:cytology / Q000378:metabolism; D053802:Tryptases
D000305:Adrenal Cortex Hormones; D015415:Biomarkers; D001798:Blood Proteins; D009195:Peroxidase; D012697:Serine Endopeptidases; C083294:chymase 2; D053818:Chymases; D053802:Tryptases