The ARF1 GTPase-activating protein: zinc finger motif and Golgi complex localization.
Hydrolysis of guanosine triphosphate (GTP) by the small guanosine triphosphatase (GTPase) adenosine diphosphate ribosylation factor-1 (ARF1) depends on a GTPase-activating protein (GAP). A complementary DNA encoding the ARF1 GAP was cloned from rat liver and predicts a protein with a zinc finger motif near the amino terminus. The GAP function required an intact zinc finger and additional amino-terminal residues. The ARF1 GAP was localized to the Golgi complex and was redistributed into a cytosolic pattern when cells were treated with brefeldin A, a drug that prevents ARF1-dependent association of coat proteins with the Golgi. Thus, the GAP is likely to be recruited to the Golgi by an ARF1-dependent mechanism.
Department of Biology, Technion-Israel Institute of Technology, Haifa, Israel.
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E
Cukierman
I
I
Huber
M
M
Rotman
D
D
Cassel
D020823:ADP-Ribosylation Factor 1; D020727:ADP-Ribosylation Factors; D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D020126:Brefeldin A; D003001:Cloning, Molecular; D003517:Cyclopentanes / Q000494:pharmacology; D003600:Cytosol / Q000378:metabolism; D018076:DNA, Complementary; D019204:GTP-Binding Proteins / Q000378:metabolism*; D020690:GTPase-Activating Proteins; D006056:Golgi Apparatus / Q000378:metabolism*; D006160:Guanosine Triphosphate / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008969:Molecular Sequence Data; D011506:Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D051381:Rats; D016335:Zinc Fingers
D003517:Cyclopentanes; D018076:DNA, Complementary; D020690:GTPase-Activating Proteins; D011506:Proteins; D020126:Brefeldin A; D006160:Guanosine Triphosphate; D019204:GTP-Binding Proteins; D020823:ADP-Ribosylation Factor 1; D020727:ADP-Ribosylation Factors