Effect of cytokines on 5-fluorouracil-mediated immunosuppression.
The effect of cytokines on 5-fluorouracil-mediated suppression of antibody response to arsanilic acid-bovine gammaglobulin (ARS-BGG) in high- and low-responding strains of mice was determined. Single i.v. injections of 5-fluorouracil strongly inhibited both primary and secondary antibody responses. The high-responding strain was found to be more sensitive to 5-fluorouracil. Restoration of suppressed IgG antibody responses to ARS-BGG was achieved in vitro by addition of either rIL-4, or rIFN gamma to the culture medium. Similarly, IgA titers recovered following the addition of exogenous rIL-5. The inhibition of IgM production, however, was not influenced by any of the cytokines tested. No significant differences were observed between experimental groups injected with antigen before or after 5-fluorouracil application. Our results suggest that the immunosuppression caused by 5-fluorouracil treatment can be abrogated by the addition of cytokines.
Department of Pathology, University of Louisville School of Medicine, KY 40292, USA.
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Vĕtvicka
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Vĕtvicková
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Sonnenfeld
D000818:Animals; D001147:Arsanilic Acid / Q000276:immunology; D002478:Cells, Cultured; D016207:Cytokines / Q000494:pharmacology*; D005260:Female; D005472:Fluorouracil / Q000009:adverse effects / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D007108:Immune Tolerance / Q000187:drug effects*; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000187:drug effects; D007166:Immunosuppressive Agents / Q000009:adverse effects / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D051379:Mice; D008805:Mice, Inbred A; D008810:Mice, Inbred C57BL; D005719:gamma-Globulins / Q000276:immunology
D016207:Cytokines; D007074:Immunoglobulin G; D007166:Immunosuppressive Agents; C088471:arsanilic acid hapten-bovine gamma globulin; D005719:gamma-Globulins; D005472:Fluorouracil; D001147:Arsanilic Acid