Multiple cloning sites carrying loxP and FRT recognition sites for the Cre and Flp site-specific recombinases.
Plasmids were constructed carrying the loxP and FRT recognition sites for the Cre and Flp site-specific recombinases, respectively, within multiple cloning sites. Vectors carrying single and tandemly repeated targets are available with various flanking restriction enzyme sites. In addition, a series of plasmids carrying both loxP and FRT sites is available. These vectors facilitate construction of target molecules for these site-specific recombinases which are becoming increasingly important tools for the in vivo manipulation of DNA.
Snaith
M R
MR
Department of Genetics, University of Cambridge, UK.
Murray
J A
JA
Boulter
C A
CA
D001483:Base Sequence; D001665:Binding Sites; D004254:DNA Nucleotidyltransferases / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D005822:Genetic Vectors; D019426:Integrases; D008969:Molecular Sequence Data; D010957:Plasmids; D015183:Restriction Mapping; D014764:Viral Proteins
D014764:Viral Proteins; C045073:Cre recombinase; D004254:DNA Nucleotidyltransferases; C045119:FLP recombinase; D019426:Integrases