Assessment of the mutagenicity of extracts of TMV-coat-protein-gene induced transgenic tomato by the umu-test.
We examined the mutagenicity of extracts (juice and ethanol extract) from a transgenic tomato that was established by transfection of a gene encoding the coat protein of tobacco mosaic virus (TMV) to the F1 hybrid between Lycopersicon esculentum LA1000 and L. peruvianum PI128650, by the umu-test with Salmonella typhimurium TA1535/pSK1002 as the test organism. The extracts showed no detectable mutagenicity. The extracts from the above-mentioned F1 hybrids and wild tomatoes and cultivars (L. peruvianum PI128650, L. peruvianum PI126944, L. pimpinellifolium LS1524, L. pimpinellifolium LA722, L. hirsutum LS503, Mini-carol, Sun-cherry, Momotaro, Odoriko, Kagome77, and Ponderosa) also showed no detectable mutagenicity.
National Food Research Institute, Ibaraki, Japan.
H
H
Shinmoto
A
A
Tomizawa
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M
Kobori
T
T
Tsushida
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K
Shinohara
D002213:Capsid / Q000235:genetics*; D018551:Solanum lycopersicum / Q000235:genetics*; D009152:Mutagenicity Tests; D010936:Plant Extracts / Q000633:toxicity*; D030821:Plants, Genetically Modified
D010936:Plant Extracts