Effects of metals on elastase from Pseudomonas aeruginosa SES-938-1.
Among the numerous virulance factors produced by Pseudomonas aeruginosa, elastase is the one most often associated with pathogenesis. In this study, effects of various metal ions on elastase from a new isolate of P. aeruginosa (Strain SES-938-1) was investigated. Crude elastase was prepared from culture supernatant via salting out by ammonium sulfate, and then desalting and concentrating the sample using a centricon microconcentrator. Activities were measured at 450 nm using N-succinyl-L-(ala)3-p-nitroanilide as the substrate. The metal chelating agents EDTA and EGTA inhibited the Pseudomonas elastase, which shows that the enzyme is a typical metalloproteinase. At a 10-mM concentration, Mn2+, Ni2+, and Zn2+ strongly inhibited the elastase, whereas Mg2+ effect was negligable. There was a gradual decrease in the enzyme activity in accordance with an increase in the concentration of metal ions.
Department of Biology, Middle East Technical University, Ankara, Turkey.
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S
Kocabiyik
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E
Ergin
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S
Turkoglu
D002614:Chelating Agents / Q000494:pharmacology; D004492:Edetic Acid / Q000494:pharmacology; D004533:Egtazic Acid / Q000494:pharmacology; D008670:Metals / Q000494:pharmacology*; D010196:Pancreatic Elastase / Q000037:antagonists & inhibitors*; D011550:Pseudomonas aeruginosa / Q000201:enzymology*; D013054:Spectrophotometry, Atomic
D002614:Chelating Agents; D008670:Metals; D004533:Egtazic Acid; D004492:Edetic Acid; D010196:Pancreatic Elastase