Effect of encapsulation on the anti-inflammatory properties of superoxide dismutase after oral administration.
Anti-inflammatory properties of free superoxide dismutase and superoxide dismutase encapsulated into liposomes, with or without ceramides, have been investigated. Two models were investigated: carrageenan paw oedema and pleurisy. Animals were fed by repeated doses, twice daily from day 1 until day 4. Evaluation consisted of measurement of paw oedema volume with determination of prostaglandin E2, thromboxane B2 and 6-keto-prostaglandin F1 alpha levels. Polymorphonuclear oxidative metabolism was evaluated by measurement of superoxide anion production. Levels of superoxide dismutase were determined in cells and pleural exudates. Higher anti-inflammatory effects were obtained after eight administrations of encapsulated forms (0.5 mg/kg) whereas free superoxide dismutase have shown no effects. Ceramides enhanced the results obtained.
Faculté de Pharmacie Paris XI, Service de Pharmacie Clinique, Chátenay Malabry, Paris, France.
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Regnault
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Roch-Arveiller
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Tissot
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Sarfati
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JP
Giroud
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Postaire
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G
Hazebroucq
D000284:Administration, Oral; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000494:pharmacology*; D002351:Carrageenan; D002417:Cattle; D002518:Ceramides / Q000494:pharmacology; D015232:Dinoprostone / Q000378:metabolism; D004337:Drug Carriers; D004339:Drug Compounding; D004487:Edema / Q000188:drug therapy / Q000473:pathology; D005122:Exudates and Transudates / Q000166:cytology / Q000378:metabolism; D007958:Leukocyte Count / Q000187:drug effects; D008081:Liposomes; D008297:Male; D009504:Neutrophils / Q000187:drug effects / Q000201:enzymology; D010998:Pleurisy / Q000188:drug therapy / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000494:pharmacology*; D013481:Superoxides / Q000378:metabolism; D013931:Thromboxanes / Q000378:metabolism
D000894:Anti-Inflammatory Agents, Non-Steroidal; D002518:Ceramides; D004337:Drug Carriers; D008081:Liposomes; D013931:Thromboxanes; D013481:Superoxides; D002351:Carrageenan; D013482:Superoxide Dismutase; D015232:Dinoprostone