Surface localization, regulation, and biologic properties of the 96-kDa alcohol/aldehyde dehydrogenase (EhADH2) of pathogenic Entamoeba histolytica.
The 96-kDa surface antigen of Entamoeba histolytica was demonstrated through extensive immunologic evaluation with monoclonal and monospecific antibodies to be identical to or an isoform of the amebic alcohol/aldehyde dehydrogenase (EhADH2). EhADH2 was secreted, excreted, or shed into the culture medium in quantities commensurate with amebic growth when studied in a novel culture system. Of importance, using RNase protection assays, specific mRNA coding for the EhADH2 gene product(s) was up-regulated by treatment of viable trophozoites with the enzyme substrate ethanol. These data provide insight into the biology of this enzyme and its regulation by appropriate stressors.
Flores
B M
BM
College of Pharmacy, Department of Pharmaceutical Sciences, Idaho State University, Pocatello 83209-8334, USA.
Stanley
S L
SL
Yong
T S
TS
Ali
M
M
Yang
W
W
Diedrich
D L
DL
Torian
B E
BE
D000426:Alcohol Dehydrogenase / Q000276:immunology / Q000378:metabolism*; D000444:Aldehyde Dehydrogenase / Q000276:immunology / Q000378:metabolism*; D000818:Animals; D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan / Q000276:immunology / Q000378:metabolism*; D000954:Antigens, Surface / Q000276:immunology / Q000378:metabolism; D003429:Cross Reactions; D003470:Culture Media; D004591:Electrophoresis, Polyacrylamide Gel; D004748:Entamoeba histolytica / Q000187:drug effects / Q000201:enzymology* / Q000276:immunology / Q000472:pathogenicity; D000431:Ethanol / Q000494:pharmacology; D015151:Immunoblotting; D007527:Isoenzymes / Q000378:metabolism*; D008970:Molecular Weight; D012333:RNA, Messenger / Q000378:metabolism; D015531:Radioimmunoprecipitation Assay; D015854:Up-Regulation
D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan; D000954:Antigens, Surface; D003470:Culture Media; D007527:Isoenzymes; D012333:RNA, Messenger; D000431:Ethanol; D000426:Alcohol Dehydrogenase; D000444:Aldehyde Dehydrogenase