Comparison of Freund's adjuvant and TiterMax in inducing anti-idiotype to idiotypic antibodies against pseudorabies virus antigens.
Freund's adjuvant (FA) and TiterMax (TM) were compared for their effectiveness in the induction of the anti-idiotypic antibodies (anti-Id or Ab2) in pigs, goats and mice against swine polyclonal anti-pseudorabies virus (PRV) antibodies (Ab1) by sequential immunization procedures. Both adjuvants had similar effects on inducing anti-swine immunoglobulin antibodies in the animals. However, high levels of the anti-Id were only generated in animals that received FA. Serological characterization of the anti-Id antisera indicated that internal image anti-Id (Ab2 beta) was generated that recognized shared idiotype (IdX) on the antibodies to PRV. The internal image Ab2 was capable of blocking the anti-PRV antibodies from binding to the PRV antigens, and their interaction with anti-PRV antibodies could be inhibited by PRV antigens.
Virology Section, Animal Diseases Research Institute, Nepean, Ont., Canada.
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Zhou
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Afshar
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000096:biosynthesis* / Q000276:immunology*; D000914:Antibodies, Viral / Q000096:biosynthesis; D000956:Antigens, Viral / Q000276:immunology*; D001666:Binding Sites, Antibody; D001667:Binding, Competitive; D005620:Freund's Adjuvant / Q000494:pharmacology*; D006041:Goats; D011558:Herpesvirus 1, Suid / Q000276:immunology*; D051379:Mice; D011060:Poloxalene / Q000494:pharmacology*; D013552:Swine
D000888:Antibodies, Anti-Idiotypic; D000914:Antibodies, Viral; D000956:Antigens, Viral; C086752:TiterMax; D011060:Poloxalene; D005620:Freund's Adjuvant