Nucleation of microtubule assembly by a gamma-tubulin-containing ring complex.
The highly conserved protein gamma-tubulin is required for microtubule nucleation in vivo. When viewed in the electron microscope, a highly purified gamma-tubulin complex from Xenopus consisting of at least seven different proteins is seen to have an open ring structure. This complex acts as an active microtubule-nucleating unit which can cap the minus ends of microtubules in vitro.
Department of Biochemistry and Biophysics, University of California at San Francisco 94143-0448, USA.
Y
Y
Zheng
M L
ML
Wong
B
B
Alberts
T
T
Mitchison
D000595:Amino Acid Sequence; D000818:Animals; D002846:Chromatography, Affinity; D008854:Microscopy, Electron; D008870:Microtubules / Q000378:metabolism* / Q000648:ultrastructure; D008969:Molecular Sequence Data; D009865:Oocytes / Q000737:chemistry; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D014404:Tubulin / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism / Q000648:ultrastructure; D014981:Xenopus
D014404:Tubulin