Acute effects of inhalation exposure to carbon monoxide on schedule-controlled operant behavior and blood carboxyhemoglobin levels in rats.
The acute effects of carbon monoxide (CO) exposure on a steady-state operant behavior (bar-pressing under a VI 60-sec schedule of food reinforcement) were repeatedly measured in (a) rats exposed to various concentrations of CO (500, 1,000, 1,500 and 2,000 ppm) for 1 h and (b) rats exposed to 1,500 ppm for different periods (1, 2 and 4 h). Measurements were made continuously before, during and after the exposure period. Abrupt cessation of the response was produced by exposure to 1,000 ppm or higher concentrations of CO. Recovery from the effects of CO exposure was observed as sudden resumption of responding during the post-exposure period. The duration of exposure required to produce response inhibition was closely correlated with the exposure concentration. The post-exposure interval required for response recovery was also correlated with the exposure concentration. This post-exposure response recovery interval, however, was constant and independent of the duration of exposure when the concentration was fixed at 1,500 ppm. In order to correlate these behavioral changes with an internal index of CO exposure, blood carboxyhemoglobin (HbCO) levels were determined under several exposure conditions corresponding to those of the behavioral observations. It was found that HbCO levels were within a certain range (33-43%) when response recovery occurred, suggesting the existence of a critical HbCO level (threshold) associated with the drastic behavioral change. Hence, these results support the view that blood HbCO is an important determinant of the acute behavioral effects of CO.
National Institute of Industrial Health, Kawasaki, Japan.
M
M
Miyagawa
T
T
Honma
M
M
Sato
H
H
Hasegawa
D000280:Administration, Inhalation; D000704:Analysis of Variance; D000818:Animals; D002248:Carbon Monoxide / Q000008:administration & dosage / Q000633:toxicity*; D002263:Carboxyhemoglobin / Q000187:drug effects* / Q000378:metabolism; D003216:Conditioning, Operant / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D011930:Reaction Time / Q000187:drug effects; D013997:Time Factors
D002248:Carbon Monoxide; D002263:Carboxyhemoglobin