[The effect of the conditions of the controlled cultivation of Neisseria meningitidis on the lipopolysaccharide yield].
The preparations of lipopolysaccharide (LPS) were obtained from N.meningitidis cells grown in synthetic nutrient medium in a fermenter. Different N.meningitidis strains, groups A, B, C, were found to give different yield of the preparation of LPS: the maximum yield was obtained from N.meningitidis cells C 133 and the minimum yield, from N.meningitidis cells B 125. The comparison of group B strains 125 and 150 showed that the former gave a higher yield of LPS. The influence of the phase of growth on the yield of the preparation of LPS obtained from N.meningitidis cells 125 was shown to be manifested under the conditions of oxygen excess; in the exponential phase the highest yield of the preparation was observed. Oxygen dissolved in the medium was found to influence the yield of the preparation of LPS: with the same content of glucose in the medium the yield of LPS was higher under the conditions of the limited concentration of oxygen; with the same concentration of oxygen in the medium the yield of LPS increased with the increase of the concentration of glucose.
Stukalova
N V
NV
Aleksakhina
N N
NN
Basnak'ian
I A
IA
D001431:Bacteriological Techniques; D003470:Culture Media; D005947:Glucose / Q000378:metabolism; D008070:Lipopolysaccharides / Q000032:analysis / Q000096:biosynthesis*; D009345:Neisseria meningitidis / Q000145:classification / Q000254:growth & development* / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism; D012703:Serotyping
D003470:Culture Media; D008070:Lipopolysaccharides; D005947:Glucose; D010100:Oxygen
Vliianie usloviĭ upravliaemogo kul'tivirovaniia Neisseria meningitidis na vykhod lipopolisakharida.