Gastric microcirculatory disturbance and behaviour of leucocytes after thermal injury: intravital observation of arteriovenous shunting channels in the gastric submucosal layer.
In order to investigate the pathogenesis of acute gastric mucosal lesion after thermal injury, microcirculatory disturbance was assessed and observation of the behaviour of leucocytes was performed. Gastric blood flow decreased at 15 min post-thermal injury, and partially improved at 2 h; however, it decreased again at 5 h post-thermal injury. Mucosal microcirculatory disturbance was observed by using vascular labelling with monastral blue B. Deposits of monastral blue B were observed centring mainly on collecting venules but were also observed in the capillaries. Submucosal microcirculatory disturbance was observed through an intravital microscope. The irregularity of the wall and segmental constriction in the venules and presence of an arteriovenous shunting channel was observed in the submucosal layer at 5 h post-thermal injury. The percentage of rolling or sticking leucocytes that passed the confluence of a prevenule and a venule were significantly increased at 5 h after thermal injury. The present study revealed depression of gastric blood flow, mucosal and submucosal microcirculatory disturbance, and a significant increase of rolling and sticking leucocytes in the peripheral part of venules after thermal injury. Leucocyte-endothelial interactions may occur under such conditions and this interaction may play an important role in inducing the microcirculatory disturbance that results in an acute gastric mucosal lesion after thermal injury. The present study also demonstrated the possibility of intravital study of gastric submucosal arteriovenous shunting channels.
Department of Surgery, Keio University School of Medicine, Tokyo, Japan.
M
M
Yoshida
D
D
Fukumura
G
G
Wakabayashi
Y
Y
Otani
A
A
Oshima
M
M
Shimazu
T
T
Kubota
K
K
Kumai
I
I
Kurose
S
S
Miura
D000818:Animals; D001163:Arteriovenous Anastomosis / Q000503:physiopathology*; D001783:Blood Flow Velocity; D005753:Gastric Mucosa / Q000098:blood supply / Q000293:injuries*; D006358:Hot Temperature / Q000009:adverse effects*; D007962:Leukocytes / Q000502:physiology*; D008297:Male; D051381:Rats; D017208:Rats, Wistar