Production of tissue inhibitor of metalloproteinases-1 (TIMP-1) by human astrocytic tumors.
Production of tissue inhibitor of metalloproteinases-1 (TIMP-1), a specific inhibitor of matrix metalloproteinases, was investigated in human astrocytic tumors (n = 15) and normal brain tissues (n = 3). Enzyme immunoassay indicated that TIMP-1 levels in the culture media of tumor explants were significantly higher in glioblastomas as compared with anaplastic astrocytomas, astrocytomas, and normal brain tissues. Immunohistochemistry using a monoclonal antibody against TIMP-1 demonstrated that TIMP-1 was detectable mainly in tumor cells, especially in glioblastoma. These results suggest that increased expression of TIMP-1 is associated with the malignant progression of astrocytic tumors.
Department of Neurosurgery, Fukui Medical School.
T
T
Nakagawa
T
T
Kubota
M
M
Kabuto
K
K
Sato
Y
Y
Arai
T
T
Kodera
D000328:Adult; D000368:Aged; D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D001254:Astrocytoma / Q000473:pathology* / Q000648:ultrastructure; D001921:Brain / Q000473:pathology* / Q000648:ultrastructure; D001932:Brain Neoplasms / Q000473:pathology* / Q000648:ultrastructure; D002465:Cell Movement / Q000187:drug effects*; D046508:Culture Techniques; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D008297:Male; D008666:Metalloendopeptidases / Q000378:metabolism*; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D012795:Sialoglycoproteins / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D012795:Sialoglycoproteins; D008666:Metalloendopeptidases