Effects of GABAergic drugs on the recovery of reflex potentials after spinal cord ischemia in cats.
Effects of GABAergic drugs on the recovery of reflex potentials after spinal cord ischemia were examined in anesthethized spinal cats. Monosynaptic reflex (MSR) and polysynaptic reflex (PSR) potentials, elicited by electrical stimulation of the tibial nerve in spinal cats, were recorded from the lumbo-sacral ventral root. The spinal reflex potentials were immediately depressed by occlusion of the thoracic aorta and the bilateral mammary arteries for 10 min. The potentials recovered gradually to the control level within 90 min after reperfusion. Pretreatment with bicuculline (0.3 mg/kg, i.v.), a GABA antagonist, or semicarbazide (200 mg/kg, i.v.), an inhibitor of GABA synthesis, accelerated the recovery of PSR potentials after the removal of the arterial occlusion. In contrast, pretreatment with aminooxyacetic acid (10 mg/kg, i.v.), an inhibitor of GABA degradation, retarded the recovery of PSR potentials, and this effect was overcome by the addition of the opioid antagonist naloxone (10 mg/kg, i.v.). These results suggest that the GABAergic system retards the recovery of PSR potentials after a brief spinal cord ischemia, which can be antagonized by naloxone.
Suzuki
T
T
Department of Pharmacology, School of Medicine, Chiba University, Japan.
Okamoto
Y
Y
Sekikawa
T
T
Nemoto
T
T
Moriya
H
H
Murayama
S
S
Nakaya
H
H
D000818:Animals; D001640:Bicuculline / Q000494:pharmacology; D002415:Cats; D005260:Female; D007511:Ischemia; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects*; D009270:Naloxone / Q000494:pharmacology; D018079:Receptors, GABA / Q000187:drug effects*; D012018:Reflex / Q000187:drug effects*; D013119:Spinal Cord Injuries; D013997:Time Factors
D018079:Receptors, GABA; D009270:Naloxone; D001640:Bicuculline