Studies on the replication of Mayaro virus grown in interferon treated cells.
Mayaro virus grown in interferon treated infected cells has been characterized with regard to its ability to replicate in vertebrate (TC7) and invertebrate (Aedes albopictus) cells. Virus purified from interferon treated TC7 cells adsorbs and penetrates to the same extent as the control virus. During infection, these virus particles caused inhibition of host protein synthesis and synthesized the same spectrum of viral proteins as normal virus. This population however, was apparently more sensitive to interferon treatment. Electron microscopy of TC7 cells showed the presence of numerous aberrant virus particles budding from the plasma membrane.
Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Centro de Ciéncias de Saúde, Brasil.
M C
MC
Rebello
M E
ME
Fonseca
J O
JO
Marinho
M A
MA
Rebello
D000330:Aedes / Q000648:ultrastructure / Q000821:virology; D000524:Alphavirus / Q000302:isolation & purification / Q000502:physiology*; D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D000077190:Interferon alpha-2; D016898:Interferon-alpha / Q000235:genetics / Q000494:pharmacology*; D011994:Recombinant Proteins; D014764:Viral Proteins / Q000096:biosynthesis; D014779:Virus Replication / Q000187:drug effects*
D000077190:Interferon alpha-2; D016898:Interferon-alpha; D011994:Recombinant Proteins; D014764:Viral Proteins