Luminol-chemiluminescence in free peritoneal cells in hemorrhagic shock in rats treated with PAF-receptor-antagonist BN 52021.
The activity of rat peritoneal cells were assessed by the phorbol mirystinian acetate (PMA)-induced luminol chemiluminescence (LCL). Results in control groups (0 - no manipulation, and I - carotid artery cannulation) were compared with those in the untreated hemorrhagic shock (group II), in the shock treated with the standard polyelectrolyte solution (PES) (group III), and in shock treated with PAF receptor-antagonist BN 52021 + PES (group IV). The maximal and the most rapid LCL was observed in the group treated with BN 52021 (group IV), while chemiluminescent response in the the untreated shock (group II) and in shock treated with PES was minimally expressed and late. The findings indicate for a rapid activation of peritoneal cells during ca 1 hour of hemorrhagic shock. This leads to exhausting their ability to the superoxide anion generation 15 minutes later. Peritoneal cells obtained from the group treated with the BN 52021 revealed a preserved ability to the respiratory burst. It can be concluded that BN 52021 effectively inhibits activation of the PC during hemorrhagic shock.
Department of Pediatric Surgery, Medical Academy of Białystok.
W
W
Debek
P
P
Gruca
L
L
Chyczewski
A
A
Gruca
D000704:Analysis of Variance; D000818:Animals; D001202:Ascitic Fluid / Q000378:metabolism*; D004224:Diterpenes; D005260:Female; D046934:Ginkgolides; D007783:Lactones / Q000494:pharmacology* / Q000627:therapeutic use; D008163:Luminescent Measurements; D008165:Luminol / Q000032:analysis; D010972:Platelet Activating Factor / Q000037:antagonists & inhibitors*; D010980:Platelet Membrane Glycoproteins / Q000037:antagonists & inhibitors*; D051381:Rats; D017208:Rats, Wistar; D011956:Receptors, Cell Surface; D043562:Receptors, G-Protein-Coupled; D012771:Shock, Hemorrhagic / Q000188:drug therapy / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate
D004224:Diterpenes; D046934:Ginkgolides; D007783:Lactones; D010972:Platelet Activating Factor; D010980:Platelet Membrane Glycoproteins; D011956:Receptors, Cell Surface; D043562:Receptors, G-Protein-Coupled; C039351:platelet activating factor receptor; D008165:Luminol; C045856:ginkgolide B; D013755:Tetradecanoylphorbol Acetate