Precise large deletions by the PCR-based overlap extension method.
The authors describe an efficient method for generating large deletions (> 200 nts) of precise length using the PCR-based method of gene splicing by overlap extension (1). This method is technically simple and less time consuming than conventional loop-out mutagenesis techniques requiring preparation of a single-stranded DNA template.
Senanayake
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SD
Department of Microbiology, University of Tennessee, Knoxville 37996-0845, USA.
Brian
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DA
D001483:Base Sequence; D003001:Cloning, Molecular; D017934:Coronavirus / Q000235:genetics; D017931:DNA Primers; D004274:DNA, Recombinant; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D016133:Polymerase Chain Reaction / Q000379:methods*; D012313:RNA / Q000737:chemistry; D012367:RNA, Viral; D017384:Sequence Deletion
D017931:DNA Primers; D004274:DNA, Recombinant; D012367:RNA, Viral; C039919:RNA, recombinant; D012313:RNA