Cloning of cDNA for rat eosinophil major basic protein.
We have determined the complete nucleotide sequence for the cDNA encoding rat eosinophil major basic protein (MBP) using the rapid amplification of cDNA ends (RACE) procedure. The deduced amino acid sequence revealed that the rat prepro-MBP has three functional domains, namely the signal peptide, the acidic peptide that contains numerous acidic amino acids, and the mature MBP, as in human and guinea pig MBP.
Department of Pathophysiological Biochemistry, Faculty of Pharmaceutical Sciences, Tohoku University, Miyagi, Japan.
T
T
Nittoh
M
M
Watanabe
H
H
Okayama
S
S
Misawa
Y
Y
Isobe
H
H
Hayashi
S
S
Mue
K
K
Ohuchi
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001798:Blood Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000096:biosynthesis* / Q000737:chemistry; D047091:Eosinophil Granule Proteins; D008969:Molecular Sequence Data; D011498:Protein Precursors / Q000235:genetics*; D051381:Rats; D012260:Ribonucleases
D001798:Blood Proteins; D018076:DNA, Complementary; D047091:Eosinophil Granule Proteins; D011498:Protein Precursors; D012260:Ribonucleases