Action of phenolic antioxidants on various active oxygen species.
The action of phenolic antioxidants, such as probucol, on various active oxygen species was investigated using luminol chemiluminescence and spin trapping with 5,5-dimethyl-1-pyrroline-N-oxide (DMPO). The various active oxygen species, including hydroxyl radicals (Fenton reaction), superoxide anions, singlet oxygen and hypochlorite ions were examined with phenolic antioxidants under aqueous and nonaqueous conditions. Probucol showed a quenching effect on both superoxide anions and hypochlorite ions in nonaqueous solution. However, it had no effect on hydroxyl radicals. alpha-Tocopherol, a natural phenolic antioxidant, showed a stronger quenching effect on superoxide anions and hypochlorite ions than probucol, and quenched hydroxyl radicals in nonaqueous solution. Furthermore, Trolox showed a quenching effect on all active oxygen species in both aqueous and nonaqueous solution. The antioxidants were studied under comparable conditions in a series of test systems and the reactivity profiles depicted as 'radar charts' which are helpful for characterizing antioxidant action.
Cynshi
O
O
Fujigotemba Research Laboratories Chugai Pharmaceutical Co., Ltd., Shizuoka, Japan.
Takashima
Y
Y
Katoh
Y
Y
Tamura
K
K
Sato
M
M
Fujita
Y
Y
D000975:Antioxidants / Q000737:chemistry; D002084:Butylated Hydroxytoluene / Q000737:chemistry; D002839:Chromans / Q000737:chemistry; D003497:Cyclic N-Oxides; D004578:Electron Spin Resonance Spectroscopy; D005609:Free Radicals; D017665:Hydroxyl Radical / Q000737:chemistry; D006997:Hypochlorous Acid / Q000737:chemistry; D008163:Luminescent Measurements; D008165:Luminol; D015394:Molecular Structure; D010100:Oxygen / Q000737:chemistry; D010636:Phenols / Q000737:chemistry*; D011341:Probucol / Q000737:chemistry; D017382:Reactive Oxygen Species; D026082:Singlet Oxygen; D012996:Solutions; D013113:Spin Labels; D014810:Vitamin E / Q000737:chemistry
D000975:Antioxidants; D002839:Chromans; D003497:Cyclic N-Oxides; D005609:Free Radicals; D010636:Phenols; D017382:Reactive Oxygen Species; D012996:Solutions; D013113:Spin Labels; D014810:Vitamin E; D026082:Singlet Oxygen; D002084:Butylated Hydroxytoluene; D017665:Hydroxyl Radical; D008165:Luminol; D006997:Hypochlorous Acid; C017245:5,5-dimethyl-1-pyrroline-1-oxide; D011341:Probucol; C010643:6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid; D010100:Oxygen