Frur mediates catabolite activation of pyruvate kinase (pykF) gene expression in Escherichia coli.
Expression of a pykF-lacZ fusion was studied as a function of the carbon source in wild-type strains and strains lacking or overproducing the FruR protein of Escherichia coli. FruR controls the response to the carbon source by repressing pykF expression more strongly under gluconeogenic than under glycolytic conditions, a phenomenon we term catabolite activation.
Department of Biology, University of California at San Diego, La Jolla 92093-0116, USA.
S A
SA
Bledig
T M
TM
Ramseier
M H
MH
Saier
D001426:Bacterial Proteins / Q000096:biosynthesis / Q000502:physiology*; D001483:Base Sequence; D004789:Enzyme Activation; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D029968:Escherichia coli Proteins; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D005943:Gluconeogenesis; D006019:Glycolysis; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics; D011770:Pyruvate Kinase / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D012097:Repressor Proteins / Q000096:biosynthesis / Q000502:physiology*; D001616:beta-Galactosidase / Q000096:biosynthesis / Q000235:genetics; D056968:Lac Repressors
D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D011770:Pyruvate Kinase; D011993:Recombinant Fusion Proteins; D012097:Repressor Proteins; D001616:beta-Galactosidase; C489416:FruR protein, E coli; C068837:FruR protein, Bacteria; D056968:Lac Repressors