Effects of testosterone buciclate on testicular and epididymal sperm functions in bonnet monkeys (Macaca radiata)
The effects of testosterone buciclate (TB), a long-acting androgen ester given i.m. at four sites (20 mg/site) on days 1 and 91 of the study period (360 days), on reproductive and hormonal parameters were evaluated in five adult male bonnet monkeys; untreated animals (n = 5) acted as controls to monitor seasonal changes in these parameters. In control animals, testicular volume remained unchanged throughout the study; sperm count, motility and gel penetrability decreased while the percentage of spermatozoa showing retention of cytoplasmic droplet and coiled tail increased in June-July (days 210-240), preceded by reduction in serum testosterone (T) levels on days 120-150 (March-April). The TB-treated animals showed reduced testicular volume (days 90-270), suppressed sperm motility and gel penetrability (days 45-240 except on day 120), decreased sperm count (days 75-270), and an increased percentage of spermatozoa showing retention of cytoplasmic droplet and coiled tail (days 45-240 except on day 120). Even though serum T levels remained elevated until day 300, these levels were within the physiological range. The changes induced by TB were reversible. The suppression of testicular and epididymal functions by TB indicates that this long-acting androgen may have the potentiality to induce and maintain reversible sterility, but further evaluation needs to be carried out to develop an appropriate dosage regimen that would prevent return to normal functions in order to develop this long-acting androgen as a hormonal male contraceptive.(ABSTRACT TRUNCATED AT 250 WORDS)
Kinger
S
S
Department of Reproductive Biology, All India Institute of Medical Sciences, New Delhi.
Pal
P C
PC
Rajalakshmi
M
M
Kumar
P K
PK
Sharma
D N
DN
Bajaj
J S
JS
D000818:Animals; D001835:Body Weight / Q000187:drug effects / Q000502:physiology; D003272:Contraceptive Agents, Male / Q000494:pharmacology* / Q000592:standards; D004822:Epididymis / Q000033:anatomy & histology / Q000166:cytology* / Q000502:physiology; D008255:Macaca radiata; D008297:Male; D011897:Random Allocation; D012661:Semen / Q000502:physiology; D013076:Sperm Count / Q000187:drug effects; D013081:Sperm Motility / Q000187:drug effects / Q000502:physiology; D013094:Spermatozoa / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D013737:Testis / Q000033:anatomy & histology / Q000166:cytology* / Q000502:physiology; D013739:Testosterone / Q000031:analogs & derivatives* / Q000097:blood / Q000494:pharmacology / Q000502:physiology / Q000592:standards
D003272:Contraceptive Agents, Male; C050320:testosterone 4-n-butylcyclohexylcarboxylic acid; D013739:Testosterone