Glycyl-histidyl-lysine interacts with the angiotensin II AT1 receptor.
Gly-His-Lys, a tripeptide isolated from human plasma that increases the growth rate of many cells, stimulated in dose-dependent fashion the activity of phosphorylase a in isolated rat hepatocytes. Such effect was associated to increases in both IP3 production and [Ca++]i. Interestingly, these effects of Gly-His-Lys were antagonized by losartan, a nonpeptide angiotensin II receptor antagonist (AT1 selective), which suggested that these receptors were involved in its effect. Binding competition experiments using the radioligand [125I][Sar1-Ile8]angiotensin II clearly indicated that Gly-His-Lys interacts with AT1 receptors. It was also observed that other histidine-containing tripeptides were also capable of interacting with these receptors.
Departamento de Bioenergética, Universidad Nacional Autónoma de México, México D. F.
J A
JA
García-Sáinz
J A
JA
Olivares-Reyes
D015059:1-Sarcosine-8-Isoleucine Angiotensin II / Q000378:metabolism; D000595:Amino Acid Sequence; D057911:Angiotensin Receptor Antagonists; D000818:Animals; D001667:Binding, Competitive; D001713:Biphenyl Compounds / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005260:Female; D006133:Growth Substances / Q000378:metabolism*; D007093:Imidazoles / Q000494:pharmacology; D007295:Inositol Phosphates / Q000378:metabolism; D008099:Liver / Q000166:cytology / Q000201:enzymology / Q000378:metabolism*; D019808:Losartan; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000378:metabolism*; D010762:Phosphorylase a / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D011945:Receptors, Angiotensin / Q000378:metabolism*; D015398:Signal Transduction; D013777:Tetrazoles / Q000494:pharmacology; D014667:Vasopressins / Q000494:pharmacology
D057911:Angiotensin Receptor Antagonists; D001713:Biphenyl Compounds; D006133:Growth Substances; D007093:Imidazoles; D007295:Inositol Phosphates; D009842:Oligopeptides; D011945:Receptors, Angiotensin; D013777:Tetrazoles; D014667:Vasopressins; C020905:glycyl-histidyl-lysine; D015059:1-Sarcosine-8-Isoleucine Angiotensin II; D010762:Phosphorylase a; D019808:Losartan; D002118:Calcium