Identification of amplified restriction fragment polymorphism (AFLP) markers tightly linked to the tomato Cf-9 gene for resistance to Cladosporium fulvum.
Using the technique of amplified restriction fragment polymorphism (AFLP) analysis, and bulked segregant pools from F2 progeny of the cross Lycopersicon esculentum (Cf9) x L. pennellii, approximately 42,000 AFLP loci for tight linkage to the tomato Cf-9 gene for resistance to Cladosporium fulvum have been screened. Analysis of F2 recombinants identified three markers which co-segregated with Cf-9. The Cf-9 gene has recently been isolated by transposon tagging using the maize transposon Dissociation (Ds). Analysis of plasmid clones containing Cf-9 shows that two of these markers are located on opposite sides of the gene separated by 15.5 kbp of intervening DNA. AFLP analysis provides a rapid and efficient technique for detecting large numbers of DNA markers and should expedite plant gene isolation by positional cloning and the construction of high-density molecular linkage maps of plant genomes.
Sainsbury Laboratory, John Innes Centre for Plant Science Research, Norwich, UK.
C M
CM
Thomas
P
P
Vos
M
M
Zabeau
D A
DA
Jones
K A
KA
Norcott
B P
BP
Chadwick
J D
JD
Jones
D001483:Base Sequence; D002874:Chromosome Mapping; D002963:Cladosporium / Q000472:pathogenicity*; D003433:Crosses, Genetic; D005784:Gene Amplification; D017343:Genes, Plant; D008040:Genetic Linkage; D005819:Genetic Markers; D015698:Genomic Library; D007113:Immunity, Innate / Q000235:genetics; D018551:Solanum lycopersicum / Q000235:genetics* / Q000382:microbiology; D008540:Meiosis; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D011995:Recombination, Genetic; D013045:Species Specificity
D005819:Genetic Markers