Coexpression of the myc gene family members in human neuroblastoma cell lines.
Members of the myc oncogene family such as c, N-, and L-myc are expressed in many malignant tumors. Expression of c-, N-, and L-myc oncogenes in 7 human neuroblastoma cell lines (GOTO, IMR-32, TGW, SCCH-26, TNB 9, NBL-S, and SK-N-SH), a human small cell lung carcinoma SBC-5 cell line, and a human monocytic leukemia THP-1-S cell line at mRNA and protein levels was studied to know the specificity of a newly developed antibody against homologous region at C-terminus of N-Myc, designated as anti pan-Myc antibody. By RT-PCR and immunoblot analysis, coexpression of three myc genes was detected in all neuroblastoma cell lines tested. c-and L-myc expression were observed that anti pan-Myc antibody recognizes c-Myc and N-Myc proteins but not L-Myc. These results indicate that neuroblastoma cells may acquire an aberrant transcriptional control system in myc family gene expression.
Department of Molecular Biology, Toho University School of Medicine, Tokyo, Japan.
H
H
Hemmi
K
K
Yamada
U H
UH
Yoon
M
M
Kato
F
F
Taniguchi
Y
Y
Tsuchida
H
H
Shimatake
D001483:Base Sequence; D018288:Carcinoma, Small Cell; D002460:Cell Line; D017931:DNA Primers; D016259:Genes, myc; D006801:Humans; D007951:Leukemia, Myeloid; D008175:Lung Neoplasms; D008969:Molecular Sequence Data; D005810:Multigene Family; D009447:Neuroblastoma; D016133:Polymerase Chain Reaction; D016271:Proto-Oncogene Proteins c-myc / Q000032:analysis / Q000096:biosynthesis; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D014407:Tumor Cells, Cultured
D017931:DNA Primers; D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger