Memory T lymphocytes' adherence to interferon gamma-activated human dermal microvascular endothelial cells via E-selectin.
The regulation of leukocytes-endothelial cells binding by biological response modifiers have an important role in determining the progression of acute and chronic inflammatory responses. In order to define the influence of E-selectin on the binding of T lymphocytes to human dermal microvascular endothelial cells (HDMEC), we examined the cell surface expression of E-selectin on HDMEC and the regulation of the binding of T lymphocytes to HDMEC by IFN-gamma. We have demonstrated that stimulation of HDMEC with IL-1 alpha or TNF alpha leads to transient E-selectin induction which disappears after 48 h, but stimulation of HDMEC with IFN-gamma resulted in delayed E-selectin induction which was seen at 48 h of incubation and persisted until 72 h after stimulation. However, stimulation with IFN-gamma failed to induce E-selectin expression on human umbilical vein endothelial cells. The delayed E-selectin expression on HDMEC by IFN-gamma coincided with the increases in T lymphocyte binding to IFN-gamma-activated HDMEC. The binding of memory T lymphocytes to IFN-gamma-activated HDMEC was greater than that of naive T lymphocytes. Anti-E-selectin antibody partially inhibited memory T lymphocyte binding to HDMEC after 48 h of stimulation with IFN-gamma. These data show that E-selectin expressions by IFN-gamma on endothelial cells are regulated in a tissue-specific fashion and that E-selectin may be important in vivo in the preferential migration of memory T lymphocytes into inflammatory sites in the skin.
Department of Dermatology, Yonsei University College of Medicine, Seoul, Korea.
K H
KH
Lee
K Y
KY
Chung
Y J
YJ
Koh
D000911:Antibodies, Monoclonal / Q000276:immunology; D002448:Cell Adhesion / Q000187:drug effects; D015815:Cell Adhesion Molecules / Q000276:immunology; D002478:Cells, Cultured; D019040:E-Selectin / Q000502:physiology*; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D007155:Immunologic Factors / Q000494:pharmacology; D007156:Immunologic Memory / Q000502:physiology*; D007371:Interferon-gamma / Q000494:pharmacology*; D008833:Microcirculation / Q000187:drug effects; D012867:Skin / Q000098:blood supply*; D013601:T-Lymphocytes / Q000187:drug effects / Q000502:physiology*
D000911:Antibodies, Monoclonal; D015815:Cell Adhesion Molecules; D019040:E-Selectin; D007155:Immunologic Factors; D007371:Interferon-gamma