A complex of the signal sequence binding protein and the SRP RNA promotes translocation of nascent proteins.
Translocation of proteins across the endoplasmic reticulum membrane is initiated by the signal recognition particle (SRP), a cytoplasmic ribonucleoprotein complex consisting of a 7S RNA and six polypeptides. To investigate the functions of the SRP components, we have tested the activities of several SRP subparticles. We show that the SRP GTPase (SRP54) alone binds a signal sequence and discriminates it from a non-signal sequence. Although SRP54 alone is unable to promote translocation, SRP54 in a complex with SRP RNA is both necessary and sufficient to promote translocation of an elongation-arrested nascent protein in a GTP-regulated manner. For co-translational translocation, additional SRP components are required. We discuss the implications of our results for the function of the Escherichia coli SRP which is homologous to the SRP54/SRP-RNA complex.
Hauser
S
S
ZMBH, Zentrum für Molekulare Biologie, Universität Heidelberg, Germany.
Bacher
G
G
Dobberstein
B
B
Lütcke
H
H
D000818:Animals; D001692:Biological Transport; D003600:Cytosol / Q000378:metabolism; D006160:Guanosine Triphosphate / Q000378:metabolism; D006801:Humans; D007425:Intracellular Membranes / Q000378:metabolism; D008861:Microsomes / Q000378:metabolism; D009154:Mutation; D011485:Protein Binding; D021382:Protein Sorting Signals / Q000378:metabolism*; D012342:RNA, Small Nuclear / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D018271:Signal Recognition Particle / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*
D021382:Protein Sorting Signals; D012342:RNA, Small Nuclear; D011994:Recombinant Proteins; D018271:Signal Recognition Particle; D006160:Guanosine Triphosphate