Dose-responses for the slowing of gastric emptying in a rodent model by glucagon-like peptide (7-36) NH2, amylin, cholecystokinin, and other possible regulators of nutrient uptake.
Several peptides have been proposed as regulators of nutrient release from the stomach and subsequent uptake from the gut. Using a phenol red gavage method, we compared the potencies of subcutaneously preinjected amylin, glucagon-like peptide-1 (7-36)amide (GLP-1), cholecystokinin octapeptide (CCK-8), gastric inhibitory peptide (GIP), glucagon, and pancreatic peptide on slowing the release of an acaloric gel from rat stomach. The latter three peptides did not fully inhibit gastric emptying at subcutaneous doses up to 100 micrograms. Amylin, GLP-1, and CCK-8 fully inhibited gastric emptying, with ED50s of 0.42 +/- 0.07, 6.1 +/- 0.12, and 8.5 +/- 0.20 nmol/kg +/- SE of log, respectively.
Amylin Pharmaceuticals, San Diego CA 92121, USA.
A A
AA
Young
B R
BR
Gedulin
T J
TJ
Rink
D000596:Amino Acids / Q000493:pharmacokinetics*; D000682:Amyloid / Q000494:pharmacology*; D000818:Animals; D002766:Cholecystokinin / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005746:Gastric Emptying / Q000187:drug effects* / Q000502:physiology; D005749:Gastric Inhibitory Polypeptide / Q000494:pharmacology; D005768:Gastrointestinal Hormones / Q000494:pharmacology*; D005782:Gels; D005934:Glucagon / Q000494:pharmacology; D052216:Glucagon-Like Peptide 1; D004763:Glucagon-Like Peptides; D005947:Glucose / Q000493:pharmacokinetics*; D058228:Islet Amyloid Polypeptide; D008297:Male; D010191:Pancreatic Polypeptide / Q000494:pharmacology; D010446:Peptide Fragments / Q000494:pharmacology*; D010637:Phenolsulfonphthalein; D051381:Rats; D017207:Rats, Sprague-Dawley
D000596:Amino Acids; D000682:Amyloid; D005768:Gastrointestinal Hormones; D005782:Gels; D058228:Islet Amyloid Polypeptide; D010446:Peptide Fragments; C055453:glucagon-like peptide 1 (7-36)amide; D005749:Gastric Inhibitory Polypeptide; D010191:Pancreatic Polypeptide; D004763:Glucagon-Like Peptides; D052216:Glucagon-Like Peptide 1; D005934:Glucagon; D002766:Cholecystokinin; D010637:Phenolsulfonphthalein; D005947:Glucose