Artificial immunization of rabbits with Hyalomma dromedarii tick-derived midgut antigen.
New Zealand white rabbits were immunized with partly fed Hyalomma dromedarii tick-derived midgut concealed antigens (supernate and pellet fractions) and Freund's complete adjuvant (FCA). The rabbits received three inoculations subcutaneously on days 0, 14 and 21 at a dose rate of 1 mg antigen per animal. The effects of the immunity induced was determined by infesting the rabbits with adult H. dromedarii ticks. In immunized rabbits a significant reduction in tick yield, engorgement weight, oviposition period, egg mass weight and percentage of egg hatchability was found. The gut supernatant antigen fraction induced the best protection in terms of reduced feeding and reproductive performance of the ticks.
Department of Veterinary Parasitology, College of Veterinary Sciences, CCS Haryana Agricultural University, Hisar, India.
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Kumar
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Kumar
D000818:Animals; D004064:Digestive System / Q000276:immunology; D005260:Female; D008297:Male; D010058:Oviposition; D011817:Rabbits; D013984:Tick Infestations / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D013987:Ticks / Q000276:immunology*; D014611:Vaccination / Q000662:veterinary