Effects of 17 beta-estradiol on calcitonin and calcitonin-gene-related peptide secretions and contents in a murine medullary thyroid carcinoma C-cell line (CA-77).
The effect of 17 beta-estradiol on calcitonin (CT) and calcitonin-gene-related peptide (CGRP) secretions in the murine CA-77 C cell line was studied after 1, 3, 5 and 6 d of treatment. The release of both CT and CGRP significantly increased 1, 3, 5 and 6 d after addition of 0.1 mumol/l estradiol alone to the culture medium. The C cell content of both peptides also increased after d of treatment with the same dose of estrogen. The enhanced CT and CGRP secretions induced by 17 beta-estradiol were not inhibited by the simultaneous addition of 5 mumol/l of all-trans-retinoic acid. Dexamethasone alone increased the release of both peptides within 6 d. However, when cells were treated simultaneously with estradiol and 1 mumol/l dexamethasone, the addition of retinoic acid blunted both the CT and CGRP secretions induced by dexamethasone. These results showed that the positive effects of 17 beta-estradiol on both CT and CGRP secretions were modulated by dexamethasone and retinoic acid.
Endocrinologie moléculaire et métabolique (EA No 1526 DRED), Université Pierre-et-Marie-Curie, Paris, France.
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Y
Lamari
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Ghorbel
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JM
Garel
D000818:Animals; D002116:Calcitonin / Q000378:metabolism*; D015740:Calcitonin Gene-Related Peptide / Q000378:metabolism*; D018276:Carcinoma, Medullary / Q000378:metabolism*; D003907:Dexamethasone / Q000494:pharmacology; D004958:Estradiol / Q000494:pharmacology*; D051379:Mice; D013964:Thyroid Neoplasms / Q000378:metabolism*; D014212:Tretinoin / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D004958:Estradiol; D014212:Tretinoin; D003907:Dexamethasone; D002116:Calcitonin; D015740:Calcitonin Gene-Related Peptide