Confocal microscopy reveals local SR calcium release in voltage-clamped cardiac cells.
Confocal microscopy during voltage-clamp depolarization of mammalian heart cells revealed distinct local [Ca2+]i-transients that resulted from Ca2+ released through sarcoplasmic reticulum (SR) release channel(s). When voltage was varied, the latency to occurrence and the relative probability of local [Ca2+]i-transients varied as predicted if SR Ca(2+)-release is linked tightly to Ca2+ flux through co-associated L-type Ca(2+)-channels.
Department of Physiology, University of Maryland at Baltimore 21201, USA.
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D000818:Animals; D002118:Calcium / Q000502:physiology*; D015220:Calcium Channels / Q000502:physiology; D004594:Electrophysiology; D006321:Heart / Q000502:physiology*; D017136:Ion Transport; D018613:Microscopy, Confocal; D051381:Rats; D017208:Rats, Wistar; D012519:Sarcoplasmic Reticulum / Q000502:physiology*
D015220:Calcium Channels; D002118:Calcium