Increased release of NO during ischemia reduces myocardial contractility and improves metabolic dysfunction.
BACKGROUND
We have reported that myocardial ischemia increases nitric oxide (NO) production. Several lines of evidence suggest that NO reduces myocardial contraction. Therefore, we tested whether endogenous NO decreases the inotropic response of the ischemic myocardium and whether endogenous NO is beneficial in the metabolic function of ischemic myocardium.


RESULTS
The left anterior descending coronary artery was perfused with blood from the left carotid artery in 72 dogs. An infusion of NG-nitro-L-arginine methyl ester (L-NAME), an inhibitor of NO synthase, did not affect fractional shortening (FS) under nonischemic conditions. After reduction of perfusion pressure so that coronary blood flow decreased to 60% of the control value, FS of the perfused area decreased, and intravenous infusion of isoproterenol increased FS. Before and during intravenous infusion of isoproterenol under conditions of coronary hypoperfusion, FS was significantly increased in the L-NAME group compared with the untreated group. Both lactate extraction ratio and the pH in coronary venous blood were significantly lower in the L-NAME-treated group than in the untreated group during coronary hypoperfusion. Infusion of L-arginine prevented the effects of L-NAME in the ischemic myocardium.


CONCLUSIONS
These results indicate that endogenous NO reduces myocardial contractile function and improves myocardial metabolic function in the ischemic heart. The myocardial energy-sparing effect as well as coronary vasodilation due to NO may be beneficial to the ischemic myocardium.
Node
K
K
First Department of Medicine, Osaka University School of Medicine, Japan.
Kitakaze
M
M
Kosaka
H
H
Komamura
K
K
Minamino
T
T
Inoue
M
M
Tada
M
M
Hori
M
M
Kamada
T
T
D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives / Q000494:pharmacology; D002122:Calcium Chloride / Q000494:pharmacology; D004285:Dogs; D006863:Hydrogen-Ion Concentration; D007545:Isoproterenol / Q000494:pharmacology; D007773:Lactates / Q000097:blood; D019344:Lactic Acid; D009200:Myocardial Contraction; D017202:Myocardial Ischemia / Q000503:physiopathology*; D009206:Myocardium / Q000378:metabolism*; D019331:NG-Nitroarginine Methyl Ester; D009569:Nitric Oxide / Q000502:physiology*
D007773:Lactates; D009569:Nitric Oxide; D019344:Lactic Acid; D001120:Arginine; D007545:Isoproterenol; D002122:Calcium Chloride; D019331:NG-Nitroarginine Methyl Ester