Tricistronic operon expression of the genes gcaD (tms), which encodes N-acetylglucosamine 1-phosphate uridyltransferase, prs, which encodes phosphoribosyl diphosphate synthetase, and ctc in vegetative cells of Bacillus subtilis.
The gcaD, prs, and ctc genes were shown to be organized as a tricistronic operon. The transcription of the prs gene, measured as phosphoribosyl diphosphate synthetase activity, and of the ctc gene, measured as beta-galactosidase activity specified by a ctc-lacZ protein fusion, were dependent on the promoter in front of the gcaD gene. Analysis of cDNA molecules prepared with gcaD-prs-ctc-specified mRNA as the template revealed an RNA transcript that encompassed all three cistrons.
Department of Biological Chemistry, University of Copenhagen, Denmark.
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Hilden
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Krath
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Hove-Jensen
D001412:Bacillus subtilis / Q000201:enzymology / Q000235:genetics*; D001483:Base Sequence; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009713:Nucleotidyltransferases / Q000235:genetics*; D009876:Operon / Q000235:genetics*; D012268:Ribose-Phosphate Pyrophosphokinase / Q000235:genetics*; D014158:Transcription, Genetic
D012268:Ribose-Phosphate Pyrophosphokinase; D009713:Nucleotidyltransferases; C023226:UDPacetylglucosamine pyrophosphorylase