Protein synthesis in eukaryotic organisms: new insights into the function of translation initiation factor eIF-3.
The pathway for initiation of protein synthesis in eukaryotic cells has been defined and refined over the last 25 years using purified components and in vitro reconstituted systems. More recently, powerful genetic analysis in yeast has proved useful in unraveling aspects of translation inherently more difficult to address by strictly biochemical approaches. One area in particular is the functional analysis of multi-subunit protein factors, termed eukaryotic initiation factors (eIFs), that play an essential role in translation initiation. eIF-3, the most structurally complex of the eIFs, has until recently eluded this approach. The identification of the yeast GCD10 gene as the structural gene for the zeta subunit of yeast eIF-3(1) and the analysis of mutant phenotypes has opened the door to the genetic dissection of the eIF-3 protein complex.
Department of Molecular and Cell Biology, University of Texas at Dallas, Richardson 75083-0688, USA.
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D000818:Animals; D004268:DNA-Binding Proteins; D005057:Eukaryotic Cells / Q000378:metabolism*; D039621:Eukaryotic Initiation Factor-3; D005656:Fungal Proteins / Q000235:genetics; D005800:Genes, Fungal; D006801:Humans; D008954:Models, Biological; D010448:Peptide Initiation Factors / Q000378:metabolism*; D014176:Protein Biosynthesis; D011494:Protein Kinases / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins
D004268:DNA-Binding Proteins; D039621:Eukaryotic Initiation Factor-3; D005656:Fungal Proteins; D010448:Peptide Initiation Factors; D029701:Saccharomyces cerevisiae Proteins; D011494:Protein Kinases