Processing, purification, and kinetic characterization of the Gag-Pol encoded retroviral proteinase of myeloblastosis associated virus expressed in E. coli.
Brynda
J
J
Department of Gene Manipulation, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
Fábry
M
M
Tichý
P J
PJ
Horejsí
M
M
Sedlácek
J
J
D000595:Amino Acid Sequence; D016282:Aspartic Acid Endopeptidases / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism*; D009189:Avian Myeloblastosis Virus / Q000201:enzymology* / Q000235:genetics; D001358:Avian Sarcoma Viruses / Q000201:enzymology* / Q000235:genetics; D003001:Cloning, Molecular / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel; D010450:Endopeptidases / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism*; D004926:Escherichia coli; D015685:Fusion Proteins, gag-pol / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism*; D015750:Genes, gag; D015751:Genes, pol; D007700:Kinetics; D008969:Molecular Sequence Data; D013728:Terminator Regions, Genetic
D015685:Fusion Proteins, gag-pol; D010450:Endopeptidases; D016282:Aspartic Acid Endopeptidases; C072891:Rous sarcoma virus protease; C036946:protease p15