Modified crush-avulsion anastomosis model on the rat femoral vein.
A crush-avulsion anastomosis model was established on the rat femoral vein. Saline or heparin was used as a luminal topical agent and was allowed to contact the damaged endothelium for 10 min. Patency and coagulation parameters were investigated for 1 week. The heparin treated group had a patency rate of 93% at 1 hr vs. 13% for the saline treated group (P < 0.001). At 7 days, the heparin treated group had an 87% patency vs. 7% for the saline-treated group (P < 0.001). Scanning electron micrography (SEM) provided evidence of the deposition of the components of early thrombosis in the crushed venous wall. In contrast, the SEM of the heparin treated group shows a paucity of any evidence of thrombus. These results indicate that the rat vein crush-avulsion model is a reliable and reproducible thrombosis model with low patency. The methods used with the topical agent may improve the patency rate in crush avulsion injuries.
Fu
K
K
Division of Plastic Surgery, Loyola University Chicago, USA.
Izquierdo
R
R
Hubbard
T
T
Fareed
J
J
D000714:Anastomosis, Surgical / Q000379:methods; D000818:Animals; D005268:Femoral Vein / Q000293:injuries* / Q000601:surgery* / Q000648:ultrastructure; D005343:Fibrinolytic Agents / Q000627:therapeutic use*; D006493:Heparin / Q000627:therapeutic use*; D008855:Microscopy, Electron, Scanning; D051381:Rats; D017207:Rats, Sprague-Dawley; D012965:Sodium Chloride / Q000627:therapeutic use; D013536:Suture Techniques; D013927:Thrombosis / Q000517:prevention & control*; D014654:Vascular Patency / Q000187:drug effects
D005343:Fibrinolytic Agents; D012965:Sodium Chloride; D006493:Heparin