[An alternative to immunofluorescence on frozen tissue for the diagnosis of sub-epidermal auto-immune bullous dermatosis].
The diagnosis of subepidermal auto-immune bullous dermatosis is usually based on immunofluorescence which demonstrates IgA, IgG, IgM or C3 on dermo-epidermal junction. However in some instance, this technique could not be performed. Here we report an alternative to immunofluorescence. It is a preembedding technique of immunolabelling using horseradish peroxidase, on fresh tissue without any freezing, before an inclusion in wax. So the observation of the labelling is possible with a standard microscope. This technique has been applied to 7 patients with various sub-epidermal auto-immune bullous dermatosis whose diagnosis was confirmed elsewhere by immunoelectron microscopy. It was as specific and sensitive than immunofluorescence whatever the bullous dermatosis.
Heller
M
M
Département d'Histologie, Embryologie et Cytogénétique de la faculté de Médecine Lariboisère Saint-Louis, Paris.
Dachez
R
R
Delestaing
G
G
Prost
C
C
D001327:Autoimmune Diseases / Q000175:diagnosis* / Q000378:metabolism; D015925:Cryopreservation; D019085:Fluorescent Antibody Technique, Direct; D006801:Humans; D007124:Immunoenzyme Techniques; D016253:Microscopy, Immunoelectron; D016612:Paraffin Embedding; D012872:Skin Diseases, Vesiculobullous / Q000175:diagnosis* / Q000378:metabolism
Une alternative à l'immunofluorescence sur tissu congelé pour le diagnostic des dermatoses bulleuses auto-immunes sous-épidermiques.