Site-directed mutagenesis of a disulfide bridge in cathepsin D: expression, activation, purification, and characterization.
Beyer
B M
BM
Department of Biochemistry and Molecular Biology, University of Florida, JHMHC, Gainesville 32610-0245, USA.
Dunn
B M
BM
D000595:Amino Acid Sequence; D000818:Animals; D002402:Cathepsin D / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism*; D003001:Cloning, Molecular; D003545:Cysteine; D004220:Disulfides; D004789:Enzyme Activation; D004926:Escherichia coli; D015870:Gene Expression; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009842:Oligopeptides / Q000378:metabolism; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism; D013379:Substrate Specificity
D004220:Disulfides; D009842:Oligopeptides; D011994:Recombinant Proteins; D002402:Cathepsin D; D003545:Cysteine