Differential gene expression in the murine thymus assayed by quantitative hybridization of arrayed cDNA clones.
High-throughput measurement of hybridization signatures obtained using complex probes prepared from poly(A)+ RNA and high-density cDNA colony filters is described. The performance of the system, elimination of artifacts, and verification of the validity of the data are discussed. cDNAs corresponding to sequences present at levels of approximately 0.01% in the complex probe can be detected. Good correlation is observed between expression profiles determined by this method and by Northern blotting. The method is applied to a preliminary investigation of differential expression in three cell types present in the murine thymus.
Centre d'Immunologie INSERM/CNRS de Marseille-Luminy, (CIML), France.
C
C
Nguyen
D
D
Rocha
S
S
Granjeaud
M
M
Baldit
K
K
Bernard
P
P
Naquet
B R
BR
Jordan
D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D015342:DNA Probes; D018076:DNA, Complementary / Q000032:analysis; D004848:Epithelium / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism; D005786:Gene Expression Regulation; D015723:Gene Library; D007739:L Cells; D051379:Mice; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics; D012339:RNA, Ribosomal, 28S / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid; D013601:T-Lymphocytes / Q000378:metabolism; D013950:Thymus Gland / Q000378:metabolism*
D015342:DNA Probes; D018076:DNA, Complementary; D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D012339:RNA, Ribosomal, 28S