Determination of vitamin K1 in serum using catalytic-reduction liquid chromatography with fluorescence detection.
A new method for the liquid chromatographic-fluorescence determination of serum vitamin K1 is described using reduction of the K-quinone to the fluorescent K-hydroquinone. The reduction reaction occurs "on-line" in the LC system using a catalytic reducer column and an alcohol mobile phase as reductant. A procedure for serum determination utilizes a liquid-liquid serum lipid extraction followed by normal-phase fractionation on a solid-phase extraction cartridge. The final measurement uses a reversed-phase (C18) separation with a ethanol-methanol mobile phase and provides a detection limit of approximately 20 pg/ml.
Analytical Chemistry Division, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA.
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MacCrehan
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Schönberger
D002384:Catalysis; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D012015:Reference Standards; D013050:Spectrometry, Fluorescence; D010837:Vitamin K 1 / Q000097:blood*
D010837:Vitamin K 1