Reactivity of anti-erythrocyte antibody induced by Babesia gibsoni infection against aged erythrocytes.
Effects of ageing treatment of erythrocytes to binding activity of the anti-erythrocyte antibody(s) in the serum of dogs infected artificially with Babesia gibsoni were examined by enzyme-linked immunosorbent assay (ELISA) using fixed cell antigen. When erythrocytes made senescent by two artificial ageing treatments, one of which was storing erythrocytes at 37 degrees C in medium for some days and the other was oxidation of erythrocytes with phenylhydrazine, reactivity of anti-erythrocyte antibody(s) in the infected serum to both types of treated erythrocytes was increased. These results suggest that such antibody(s) could play a role as one of the causes of anemia in canine babesiosis.
Department of Parasitology, Nippon Veterinary and Animal Science University, Tokyo, Japan.
T
T
Morita
H
H
Saeki
S
S
Imai
T
T
Ishii
D000740:Anemia / Q000150:complications / Q000276:immunology*; D000818:Animals; D001323:Autoantibodies / Q000276:immunology*; D001403:Babesia / Q000276:immunology; D001404:Babesiosis / Q000150:complications / Q000276:immunology*; D016922:Cellular Senescence; D004283:Dog Diseases / Q000276:immunology* / Q000469:parasitology; D004285:Dogs; D004912:Erythrocytes / Q000276:immunology*
D001323:Autoantibodies