Analysis of the distal 5' region of the human CYP17 gene.
In order to search for additional regulatory elements in the human CYP17 (steroid 17 alpha-hydroxylase) gene and to compare it with potential regulatory elements in bovine CYP17 genes, 3.5 kb of 5' flanking region of CYP17 was cloned and analyzed. The newly acquired sequence was shown to be a highly defective copy of the human endogenous retrovirus HERV-K family. This retroviral sequence was itself interrupted by a novel element, a low copy number repeat occurring about 20 times in the human genome, including a known copy in the human catechol-O-methyltransferase gene. A reanalysis of the entire 5' flanking region of human CYP17 indicates that only the 300 bp immediately distal to the promoter is of unique sequence; other regulatory sequences, including any that are similar to the upstream region of the bovine genes, are unlikely to occur within 5.5 kb of the promoter.
Department of Biochemistry and Molecular Biology, Medical College of Georgia, Augusta, USA.
S S
SS
Maghsoudlou
T R
TR
Hughes
P J
PJ
Hornsby
D000446:Aldehyde-Lyases / Q000235:genetics*; D000818:Animals; D002417:Cattle; D003001:Cloning, Molecular; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D006801:Humans; D011401:Promoter Regions, Genetic; D012045:Regulatory Sequences, Nucleic Acid; D012190:Retroviridae / Q000235:genetics; D013254:Steroid 17-alpha-Hydroxylase
D003577:Cytochrome P-450 Enzyme System; D013254:Steroid 17-alpha-Hydroxylase; D000446:Aldehyde-Lyases