The product of Zfp59 (Mfg2), a mouse gene expressed at the spermatid stage of spermatogenesis, accumulates in spermatozoa nuclei.
We have described previously three mouse multifinger coding genes (C. Passananti et al., Proc. Natl. Acad. Sci. USA, 86: 9417-9421, 1989). We have analyzed the expression of one of them, termed Mfg2 and renamed Zfp59, and demonstrated that Zfp59 mRNA and its translation product are present in specific stages of mouse spermatogenesis. The predicted amino acid sequence of the Zfp59 protein has been found to consist of 16 zinc-finger motifs clustered at the COOH terminus and subdivided into two groups by a degenerate finger motif. At its NH2 terminus, Zfp59 shares with other members of the zinc finger gene family two additional conserved amino acid modules A and B, described as either FAX, KRAB, or FPB domains. By means of Northern blot, Western blot analysis, and immunohistochemical localization, Zfp59 mRNA and its translation product were shown to be synthesized specifically during the postmeiotic phase of male germ line differentiation. By immunoelectron microscopy, the Zfp59 protein has been shown to accumulate in the nuclei of mature sperms in association with the nuclear matrix.
Dipartimento di Biopatologia Umana, Sezione di Biologia Cellulare, Università di Roma La Sapienza, Italy.
C
C
Passananti
N
N
Corbi
M G
MG
Paggi
M A
MA
Russo
M
M
Perez
F
F
Cotelli
M
M
Stefanini
P
P
Amati
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002467:Cell Nucleus / Q000378:metabolism*; D004268:DNA-Binding Proteins; D015870:Gene Expression; D015151:Immunoblotting; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D016253:Microscopy, Immunoelectron; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism*; D013087:Spermatids / Q000378:metabolism*; D013091:Spermatogenesis / Q000235:genetics*; D013094:Spermatozoa / Q000378:metabolism* / Q000648:ultrastructure; D016335:Zinc Fingers / Q000235:genetics*
D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D012333:RNA, Messenger; C097678:Zfp59 protein, mouse