MK-801 decreases striatal and cortical GAD65 mRNA levels.
Levels of mRNAs encoding for the two isoforms of glutamate decarboxylase, GAD67 and GAD65, were quantified in the rat striatum and cerebral cortex following single injections of the non-competitive NMDA receptor antagonist MK-801. Acute MK-801 induced a dose-dependent decrease in GAD65 mRNA levels in ventral sectors of the dorsal striatum. No statistically significant changes in GAD67 mRNA levels were measured in the same conditions. In the frontal and cingulate cortex, GAD65 but not GAD67 mRNA levels were significantly decreased after a single high dose of MK-801 (0.1 mg kg-1). These results suggest that NMDA receptors are involved in the transcriptional regulation of the GABA synthesizing enzyme, GAD65.
Centre de Recherche en Neurobiologie, Hôpital de l'Enfant-Jésus, Québec, Canada.
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Laprade
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Soghomonian
D000704:Analysis of Variance; D000818:Animals; D002540:Cerebral Cortex / Q000187:drug effects* / Q000378:metabolism; D003342:Corpus Striatum / Q000187:drug effects* / Q000378:metabolism; D016291:Dizocilpine Maleate / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology*; D005968:Glutamate Decarboxylase / Q000235:genetics*; D007527:Isoenzymes / Q000235:genetics*; D008297:Male; D008845:Microinjections; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D016194:Receptors, N-Methyl-D-Aspartate / Q000037:antagonists & inhibitors
D018691:Excitatory Amino Acid Antagonists; D007527:Isoenzymes; D016760:Proto-Oncogene Proteins c-fos; D012333:RNA, Messenger; D016194:Receptors, N-Methyl-D-Aspartate; D016291:Dizocilpine Maleate; D005968:Glutamate Decarboxylase