Cloning and functional expression of rat CLC-5, a chloride channel related to kidney disease.
We have cloned a novel member of the CLC chloride channel family from rat brain, rCLC-5. The cDNA predicts a 83-kDa protein belonging to the branch including CLC-3 and CLC-4, with which it shares approximately 80% identity. Expression of rCLC-5 in Xenopus oocytes elicits novel anion currents. They are strongly outwardly rectifying and have a conductivity sequence of NO3- > Cl- > Br- > I- >> glutamate-. Although CLC-5 has consensus sites for phosphorylation by protein kinase A, raising the intracellular cAMP concentration had no effect on these currents. Currents were also unchanged when rCLC-5 was coexpressed with rCLC-3 and rCLC-4, either singly or in combination. rCLC-5 is expressed predominantly in kidney and also in brain, lung, and liver. Along the nephron, rCLC-5 message is detectable in all tubule segments investigated, but expression in the glomerulus and the S2 segment of the proximal tubule is low.
Steinmeyer
K
K
Center for Molecular Neurobiology, Hamburg University, Federal Republic of Germany.
Schwappach
B
B
Bens
M
M
Vandewalle
A
A
Jentsch
T J
TJ
D000818:Animals; D001483:Base Sequence; D018118:Chloride Channels / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D000242:Cyclic AMP / Q000378:metabolism; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism; D017931:DNA Primers; D018076:DNA, Complementary; D007674:Kidney Diseases / Q000235:genetics*; D008969:Molecular Sequence Data; D010766:Phosphorylation; D051381:Rats; D017386:Sequence Homology, Amino Acid; D014981:Xenopus
C097503:CLC-5 chloride channel; D018118:Chloride Channels; D017931:DNA Primers; D018076:DNA, Complementary; D000242:Cyclic AMP; D017868:Cyclic AMP-Dependent Protein Kinases