Enhanced expression of TGF-beta 1 by peritoneal macrophages in CAPD patients.
Peritoneal macrophages are the predominant cells in peritoneal dialysate. To clarify the role of the macrophage in fibrosis of the peritoneum in long-term continuous ambulatory peritoneal dialysis (CAPD) patients, we studied the transforming growth factor beta (TGF-beta 1) mRNA expression by peritoneal macrophages from peritoneal dialysis patients. Macrophages were obtained when the catheter was inserted and one month later. TGF-beta 1 mRNA was measured by slot-blot hybridization using a cDNA probe. The result was that TGF-beta 1 expression was significantly higher in macrophages from peritoneal dialysate than that of macrophages obtained during catheter insertion. This suggests that peritoneal macrophages play a role in the pathogenesis of fibrosis of peritoneum in peritoneal dialysis patients. It also suggests that peritoneal macrophages in dialysate have been somewhat activated.
Wang
T
T
National Institute of Kidney, Sun Yat-sen University of Medical Sciences, Guangzhou, People's Republic of China.
Ghen
Y G
YG
Ye
R G
RG
Mai
W Y
WY
Zhen
Z H
ZH
Li
H Q
HQ
D001345:Autoradiography; D005355:Fibrosis / Q000473:pathology / Q000503:physiopathology; D006801:Humans; D017737:Macrophages, Peritoneal / Q000378:metabolism* / Q000502:physiology; D009693:Nucleic Acid Hybridization; D010531:Peritoneal Dialysis, Continuous Ambulatory; D010537:Peritoneum / Q000473:pathology; D012333:RNA, Messenger / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000235:genetics* / Q000378:metabolism
D012333:RNA, Messenger; D016212:Transforming Growth Factor beta