Pyrimidine moiety of thiamin is biosynthesized from pyridoxine and histidine in Saccharomyces cerevisiae.
The isotopes of [6-13C]- and [5'-2H2]pyridoxine were incorporated efficiently into the pyrimidine moiety of thiamin in S. cerevisiae. The mass fragmentation pattern showed that the C-6 and H-5' atoms of pyridoxine were incorporated into the C-6, and H-5' atoms of the pyrimidine, respectively. These findings, along with out previous results, show that the C-2', C-2, N-1, C-6, C-5, and C-5' unit of the pyrimidine are derived from the C-2', C-2, N-1, C-6, C-5 and C-5' unit of pyridoxine, and that pyrimidine is biosynthesized from pyridoxine and histidine.
Faculty of Pharmaceutical Sciences, Mukogawa Women's University, Hyogo, Japan.
K
K
Tazuya
C
C
Azumi
K
K
Yamada
H
H
Kumaoka
D002247:Carbon Isotopes; D003903:Deuterium; D008401:Gas Chromatography-Mass Spectrometry; D006639:Histidine / Q000378:metabolism*; D007553:Isotope Labeling / Q000379:methods; D011736:Pyridoxine / Q000378:metabolism*; D011743:Pyrimidines / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D013831:Thiamine / Q000096:biosynthesis*
D002247:Carbon Isotopes; D011743:Pyrimidines; D006639:Histidine; D003903:Deuterium; D011736:Pyridoxine; D013831:Thiamine