Use of specific immunoradiometric assay to determine preterm neonatal insulin-glucose relations.
Highly specific immunoradiometric assays were used to measure plasma concentrations of insulin, proinsulin, and 32-33 split proinsulin in neonates (n = 16). Neonatal plasma insulin concentrations were high relative to blood glucose concentrations and compared with adult insulin-glucose relations. Concentrations of proinsulin and 32-33 split proinsulin together accounted for 34-70% of the total concentration of insulin and pro-peptides. This study confirms the need to use a specific assay and neonatal reference data in the diagnosis of neonatal hyperinsulinism, and shows that neonatal pancreatic beta cell function may differ from that of older subjects.
Institute of Child Health, Liverpool University.
J M
JM
Hawdon
M
M
Hubbard
C N
CN
Hales
P M
PM
Clark
D001786:Blood Glucose; D005865:Gestational Age; D006801:Humans; D015592:Immunoradiometric Assay; D007231:Infant, Newborn; D007234:Infant, Premature / Q000097:blood*; D007328:Insulin / Q000097:blood*; D011384:Proinsulin / Q000097:blood; D011498:Protein Precursors / Q000097:blood
D001786:Blood Glucose; D007328:Insulin; D011498:Protein Precursors; C070703:proinsulin, des(31,32)-; D011384:Proinsulin