Nitroprusside inhibition of platelet function is transient and reversible by catecholamine priming.
BACKGROUND
The time course and reversibility of sodium nitroprusside's in vivo inhibition of platelet function are unclear.


METHODS
Platelet aggregation and P-selectin expression as measures of platelet dense and alpha-granule release, respectively, were examined before and after administration of sodium nitroprusside (18 mg) to human volunteers and in in vitro studies. Hypotension occurring with sodium nitroprusside administration was treated with intravenous crystalloid and/or phenylephrine.


RESULTS
Compared with preinfusion studies, platelet aggregation to epinephrine was significantly inhibited immediately and 4 min after discontinuation of the sodium nitroprusside infusion but returned to baseline at 8 and 12 min after discontinuing sodium nitroprusside. However, both dense and alpha-granule release to adenosine diphosphate after in vivo sodium nitroprusside were never significantly inhibited even at the time when sodium nitroprusside infusion was maximal. In contrast to our in vivo findings, in vitro incubation of platelet-rich plasma with sodium nitroprusside resulted in significant inhibition of dense and alpha-granule release to adenosine diphosphate. These in vitro inhibitory effects of sodium nitroprusside were reversed by pretreatment with epinephrine but not phenylephrine.


CONCLUSIONS
In normal volunteers, sodium nitroprusside inhibits platelet aggregation to epinephrine but not adenosine diphosphate; inhibition was reversed within 8-12 min after discontinuing sodium nitroprusside. Sodium nitroprusside in vitro inhibition of platelet function to adenosine diphosphate was reversed by epinephrine pretreatment. Because of the rapid reversibility of its antiplatelet effect, sodium nitroprusside may be clinically useful even when there is the potential for impaired coagulation.
Department of Anesthesiology, Yale University School of Medicine, New Haven, Connecticut 06520-8051, USA.
S N
SN
Harris
C S
CS
Rinder
H M
HM
Rinder
J B
JB
Tracey
B R
BR
Smith
R
R
Hines
D000244:Adenosine Diphosphate / Q000494:pharmacology; D001792:Blood Platelets / Q000187:drug effects*; D015550:Cell Degranulation / Q000187:drug effects; D004837:Epinephrine / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D009569:Nitric Oxide / Q000494:pharmacology; D009599:Nitroprusside / Q000494:pharmacology*; D019007:P-Selectin / Q000378:metabolism; D010974:Platelet Aggregation / Q000187:drug effects*; D013997:Time Factors; D014665:Vasodilator Agents / Q000494:pharmacology*
D019007:P-Selectin; D014665:Vasodilator Agents; D009599:Nitroprusside; D009569:Nitric Oxide; D000244:Adenosine Diphosphate; D004837:Epinephrine