Effect of neonatal thymectomy on murine small intestinal intraepithelial lymphocytes expressing T cell receptor alpha beta and "clonally forbidden V beta s".
Neonatal thymectomy NTX performed on Day 3 of C3H mice causes over a 50% reduction in small intestinal intraepithelial lymphocytes (IEL) expressing the alpha beta T cell receptor (TCR) when analyzed at 10 weeks of age. Furthermore, this reduction is most notable in alpha beta TCR IEL expressing the CD8 alpha homodimer and no Thy 1 marker. This is in direct contradiction to the present theory that alpha beta TCR IEL expressing these markers are thymic independent. Evaluation of V beta expression shows a relative increase in V beta s which are clonally forbidden (V beta s 3, 5, 11), and which is consistent with the extrathymic origin of these particular IEL.
Department of Immunology, Kyushu University, Fukuoka, Japan.
T
T
Lin
H
H
Takimoto
G
G
Matsuzaki
K
K
Nomoto
D000818:Animals; D000831:Animals, Newborn; D016827:CD8 Antigens / Q000032:analysis*; D002454:Cell Differentiation; D017637:Clonal Deletion; D007413:Intestinal Mucosa / Q000276:immunology* / Q000473:pathology; D051379:Mice; D008809:Mice, Inbred C3H; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics*; D018800:Thy-1 Antigens / Q000032:analysis; D013934:Thymectomy
D016827:CD8 Antigens; D016693:Receptors, Antigen, T-Cell, alpha-beta; D018800:Thy-1 Antigens