Molecular cloning and sequence analysis of striped bass (Morone saxatilis) gonadotrophin-I and -II subunits.
Two types of cDNA, each encoding a different beta-subunit of striped bass (Morone saxatilis, Teleostei) gonadotrophins (GTH-I beta and GTH-II beta), as well as the glycoprotein alpha-subunit, were cloned by screening a striped bass pituitary cDNA library. The probes used for screening the library were cloned cDNA fragments, generated by PCR amplification of reverse-transcribed mRNA obtained from two pituitaries. The nucleotide sequences of the alpha-subunit, GTH-I beta and GTH-II beta are 626, 524 and 580 bases long, encoding peptides of 117, 120 and 147 amino acids respectively. Striped bass GTH-I beta and GTH-II beta share a sequence identity of 48% at the nucleic acid level, and 30% at the amino acid level. A cluster analysis of vertebrate pituitary glycoprotein beta-subunits suggests that teleost GTH-II beta is more closely related to tetrapod LH than to FSH. Administration of gonadotrophin-releasing hormone analogue ([D-Ala6,Pro9Net]-LHRH) to juvenile striped resulted in ten-, two- and fivefold increases in the expression of the alpha-subunit, GTH-I beta and GTH-II beta respectively. These results suggest that each of the GTH subunits is differentially regulated, and further corroborate the functional duality of teleost gonadotrophins.
Hassin
S
S
Center of Marine Biotechnology, University of Maryland 21202, USA.
Elizur
A
A
Zohar
Y
Y
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001492:Bass / Q000235:genetics*; D015152:Blotting, Northern; D003001:Cloning, Molecular; D017931:DNA Primers; D018076:DNA, Complementary; D015292:Glycoprotein Hormones, alpha Subunit / Q000235:genetics; D006065:Gonadotropins, Pituitary / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid
D017931:DNA Primers; D018076:DNA, Complementary; D015292:Glycoprotein Hormones, alpha Subunit; D006065:Gonadotropins, Pituitary; C097582:gonadotropin I beta-subunit, bass; C097583:gonadotropin II beta subunit, bass