t(8;21) detection by RT-PCR: an improved primer set convenient for the routine laboratory.
A new set of primers to detect t(8;21) by RT-PCR have been designed. These primers give a more specific PCR amplification than the original primers and can be used with a cycling regime that is already in use for a wide range of other PCR methods in the routine haematology laboratory.
Department of Haematology, Derriford Hospital, Plymouth.
C
C
Lynas
D
D
Howe
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M
Sweeney
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M
Wagstaff
D001483:Base Sequence; D002891:Chromosomes, Human, Pair 21; D002898:Chromosomes, Human, Pair 8; D017931:DNA Primers / Q000235:genetics; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000502:physiology*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D014178:Translocation, Genetic
D017931:DNA Primers