[An alternative to immunofluorescence on frozen tissue for the diagnosis of sub-epidermal auto-immune bullous dermatosis].
The diagnosis of subepidermal auto-immune bullous dermatosis is usually based on immunofluorescence which demonstrates IgA, IgG, IgM or C3 on dermo-epidermal junction. However in some instance, this technique could not be performed. Here we report an alternative to immunofluorescence. It is a preembedding technique of immunolabelling using horseradish peroxidase, on fresh tissue without any freezing, before an inclusion in wax. So the observation of the labelling is possible with a standard microscope. This technique has been applied to 7 patients with various sub-epidermal auto-immune bullous dermatosis whose diagnosis was confirmed elsewhere by immunoelectron microscopy. It was as specific and sensitive than immunofluorescence whatever the bullous dermatosis.
Département d'Histologie, Embryologie et Cytogénétique de la faculté de Médecine Lariboisère Saint-Louis, Paris.
M
M
Heller
R
R
Dachez
G
G
Delestaing
C
C
Prost
D001327:Autoimmune Diseases / Q000175:diagnosis* / Q000378:metabolism; D015925:Cryopreservation; D019085:Fluorescent Antibody Technique, Direct; D006801:Humans; D007124:Immunoenzyme Techniques; D016253:Microscopy, Immunoelectron; D016612:Paraffin Embedding; D012872:Skin Diseases, Vesiculobullous / Q000175:diagnosis* / Q000378:metabolism
Une alternative à l'immunofluorescence sur tissu congelé pour le diagnostic des dermatoses bulleuses auto-immunes sous-épidermiques.