Induction of antigen-specific IgE response in murine lymphocytes by IL-10.
When murine spleen cells that had been primed with trinitrophenyl-keyhole limpet hemocyanin (TNP-KLH) were stimulated in vitro with the same antigen, anti-TNP IgE, as well as anti-TNP IgM and IgG1, was secreted into the culture medium. On the other hand, anti-TNP IgM and IgG1 were produced, but anti-TNP IgE secretion was negligible when the carrier (KLH)-primed spleen cells were cultured with the hapten-carrier antigen (TNP-KLH) under the same conditions. Anti-TNP Ig responses in the latter cultures are thought to reflect the interaction between normal TNP-specific B cells and KLH-primed helper T cells. By using this culture system, we investigated the requirements of exogenous cytokines for inducing anti-TNP IgE response. The addition of interleukin-4 (IL-4), that is known to induce IgE response in LPS-stimulated murine B cells, failed to elicit anti-TNP IgE response. The combination of IL-4 with IL-2 and/or IL-5 was also ineffective. Interestingly, a significant level of anti-TNP IgE was induced when IL-10, another cytokine from type 2 helper T cells, was added to the culture. Although IL-10 enhanced the production of anti-TNP IgM and IgG1, as well as that of anti-TNP IgE, the rate of enhancement was at least 3-fold higher in the IgE response than in the IgM and IgG1 responses. Simultaneous addition of IL-4, IL-5 or IL-13 with IL-10 did not augment but rather reduced the enhancing effects of IL-10. IL-10 did not further stimulate the spontaneous secretion of IgE from antigen-primed B cells.(ABSTRACT TRUNCATED AT 250 WORDS)
Department of Biotechnology, Faculty of Engineering, Okayama University, Japan.
H
H
Ohmori
T
T
Kanda
T
T
Takai
M
M
Hikida
D000818:Animals; D000918:Antibody Specificity / Q000187:drug effects*; D002478:Cells, Cultured; D000939:Epitopes / Q000187:drug effects; D006241:Haptens; D006433:Hemocyanins / Q000276:immunology; D007073:Immunoglobulin E / Q000096:biosynthesis*; D016753:Interleukin-10 / Q000494:pharmacology*; D008214:Lymphocytes / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism*; D008297:Male; D051379:Mice; D013154:Spleen / Q000166:cytology; D014301:Trinitrobenzenes / Q000276:immunology
D000939:Epitopes; D006241:Haptens; D014301:Trinitrobenzenes; C054401:trinitrophenyl keyhole limpet hemocyanin; D016753:Interleukin-10; D007073:Immunoglobulin E; D006433:Hemocyanins