Chromosomal loci of 50 human keratinocyte cDNAs assigned by fluorescence in situ hybridization.
The chromosomal loci of expressed genes provide useful information for a candidate gene approach to the genes responsible for genetic diseases. A large set of randomly isolated cDNAs catalogued by partial sequencing can serve as a resource for accessing and isolating these disease genes. Using fluorescence in situ hybridization, we examined the chromosomal loci of 217 human keratinocyte-derived cDNAs, with independent novel sequence tags at the 3' end region. Among them, we determined the loci of 50 cDNAs. Single-pass sequencing of these from the 5' ends indicated that 39 cDNAs still can be produced for new genes. These cDNAs with identified chromosomal loci are powerful tools that can be used to help elucidate the genes responsible for hereditary skin disorders.
Department of Dermatology, Kyoto Prefectural University of Medicine, Japan.
Y
Y
Morishima
T
T
Ariyama
K
K
Yamanishi
T
T
Abe
E
E
Ueda
H
H
Yasuno
J
J
Inazawa
D002874:Chromosome Mapping; D018076:DNA, Complementary / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D015603:Keratinocytes / Q000378:metabolism*; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000096:biosynthesis / Q000302:isolation & purification; D012871:Skin Diseases / Q000235:genetics
D018076:DNA, Complementary; D012333:RNA, Messenger