Use of UV photography to identify aflatoxin-producing strains of Aspergillus flavus and A. parasiticus.
UV photography in glucose, yeast extract (GY) agar medium was tested as a simple and rapid method for the distinction of aflatoxin-positive from aflatoxin-negative strains of Aspergillus flavus and A. parasiticus. In the UV photographs aflatoxin-producing moulds were identified as grey or black colonies, whereas aflatoxin-nonproducing moulds appeared as white colonies. Of the aflatoxin-positive strains detected by the UV photographic method, 10% was confirmed by extraction of the GY agar medium and mould mycelium in chloroform, extracts which were analysed subsequently using thin-layer chromatography. Confirmation of aflatoxigenic strains was achieved by biosynthesis on liquid medium yeast extract sucrose (YES) broth.
Department of Microbiology, Faculty of Pharmacy and Biochemistry, University of Zagreb, Croatia.
Z
Z
Cvetnić
S
S
Pepeljnjak
D000348:Aflatoxins / Q000096:biosynthesis*; D001230:Aspergillus / Q000145:classification* / Q000254:growth & development / Q000378:metabolism*; D001231:Aspergillus flavus / Q000145:classification / Q000254:growth & development / Q000378:metabolism*; D010781:Photography / Q000379:methods; D013045:Species Specificity; D014466:Ultraviolet Rays
D000348:Aflatoxins