Common clonal chromosome aberrations in cytokine-dependent continuous human T-lymphocyte cell lines.
Antigen-mediated T-cell proliferation is a transient phenomenon. Like other somatic cells, T lymphocytes generally show replicative senescence in vitro. However, we here show that cytokine-dependent continuous (immortal) T-cell lines can be established from skin biopsy specimens of inflammatory skin diseases. Continuous growth can be obtained by culturing T cells in medium supplemented with interleukin-2 and interleukin-4, but without antigen or antigen-presenting cells added. Loss of the T-cell antigen receptor complex is observed in some of the continuous T-cell lines. Most T-cell lines develop clonal chromosome aberrations during continuous growth. Aberrations for chromosomes 1, 2, 8, 16, and 18 are most commonly observed.
Kaltoft
K
K
Department of Human Genetics, Marselisborg University Hospital, Aarhus, Denmark.
Hansen
B H
BH
Pedersen
C B
CB
Pedersen
S
S
Thestrup-Pedersen
K
K
D002455:Cell Division; D002460:Cell Line; D002869:Chromosome Aberrations; D016207:Cytokines / Q000494:pharmacology*; D003872:Dermatitis / Q000473:pathology; D006801:Humans; D016130:Immunophenotyping; D007376:Interleukin-2 / Q000494:pharmacology; D015847:Interleukin-4 / Q000494:pharmacology; D016410:Lymphoma, T-Cell, Cutaneous / Q000235:genetics* / Q000473:pathology; D012867:Skin; D012878:Skin Neoplasms / Q000235:genetics* / Q000473:pathology; D013601:T-Lymphocytes / Q000276:immunology / Q000648:ultrastructure*; D014407:Tumor Cells, Cultured
D016207:Cytokines; D007376:Interleukin-2; D015847:Interleukin-4