Application of a new perchloric acid treatment method to measure endotoxin by an endotoxin-specific chromogenic Limulus test in neonatal septicemia.
The endotoxin in blood was measured to establish both the cut-off value and to detect Gram-negative septicemia. We employed a new perchloric acid treatment method using an endotoxin-specific chromogenic Limulus test (Endospecy test). The cut-off value of endotoxin in blood was 11.2 pg/mL. All cases of septicemia (n = 7) showed high values of endotoxin. Three cases were Group B streptococci, and two cases were Escherichia coli. The others were showed to be negative in blood cultures. The paired values of endotoxin titers during a 48 h interval were useful to evaluate the effectiveness of antibiotics.
Department of Neonatology, Morioka Red Cross Hospital, Japan.
H
H
Suda
C
C
Moroi
K
K
Inada
S
S
Chida
T
T
Fujiwara
M
M
Yoshida
D016470:Bacteremia / Q000175:diagnosis*; D004731:Endotoxins / Q000097:blood*; D006801:Humans; D007231:Infant, Newborn; D008033:Limulus Test / Q000379:methods*; D010472:Perchlorates; D011237:Predictive Value of Tests
D004731:Endotoxins; D010472:Perchlorates