Alteration by EGTA of the human red cell Ca(2+)-ATPase.
The effect of EGTA on Ca(2+)-ATPase activity was studied in fragmented membranes and solubilized preparations from human red cells. A dual action was found. At low concentrations (0.1-1 mM), EGTA increased Ca2+ affinity without affecting Vmax. By contrast, at high concentrations (5-10 mM), EGTA was inhibitory. Both effects were partially reversible.
Instituto de Biología Experimental, Facultad de Ciencias, Universidad Central de Venezuela, Caracas, Venezuela.
P J
PJ
Romero
V
V
Salas
D000252:Calcium-Transporting ATPases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004533:Egtazic Acid / Q000494:pharmacology*; D004912:Erythrocytes / Q000187:drug effects* / Q000201:enzymology; D006801:Humans
D004533:Egtazic Acid; D000252:Calcium-Transporting ATPases