[The effectiveness of perfluorotributylamine/pluronic F-68 stem-emulsion (FC43se) against xenograft rejection in the guinea pig-to-rat model].
The administration of perfluorotributylamine/pluronic F-68 stem-emulsion (FC43se), a fluorine compound emulsion, to a rodent discordant xeno-transplantation (dXT) model led to the discovery of the inhibitory effect of this compound against hyperacute rejection (HAR). When a guinea pig heart was transplanted to a rat administered with FC43se (10 microliters/g body weight), rhythmic beating was maintained for 1110 +/- 111.1 min (mean +/- SD; n = 8) whereas the untreated heart continued to beat for 15.5 +/- 6.6 min (mean +/- SD; n = 8). After 720 min of re-beating; pathologic changes observed in the untreated group, such as multiple thrombosis in the coronary vessels, were not observed in the FC43se-administered group by light and electron microscopic examination, and endothelial cells were well preserved. On the other hand, scattered necroses of the myocardium were observed and lymphocytic infiltration was demonstrated in the interstitium. We concluded that FC43se possessed a HAR inhibitory effect by inhibiting thrombus formation in the xeno-graft heart. Using this model, we speculated that action in the vessels of the graft heart (intravascular HAR) caused thrombus formation in the vessels. On the other hand, extravascular HAR causes myocardial necrosis more slowly, not resulting in cardiac arrest in the short term.
First Department of Surgery, Hiroshima University School of Medicine, Japan.
S
S
Wada
H
H
Kajihara
H
H
Murakami
S
S
Mukai
Y
Y
Kurisu
T
T
Sueda
Y
Y
Matsuura
D000208:Acute Disease; D000818:Animals; D001802:Blood Substitutes / Q000627:therapeutic use*; D003328:Coronary Thrombosis / Q000517:prevention & control; D004655:Emulsions; D005466:Fluorocarbons / Q000627:therapeutic use*; D006084:Graft Rejection / Q000517:prevention & control*; D006168:Guinea Pigs; D016027:Heart Transplantation; D008297:Male; D009206:Myocardium / Q000473:pathology*; D009336:Necrosis; D051381:Rats; D017208:Rats, Wistar; D014183:Transplantation, Heterologous
D001802:Blood Substitutes; D004655:Emulsions; D005466:Fluorocarbons; C008807:perfluorotributylamine