Albumin improves islet isolation: specific versus nonspecific effects.
1. BSA-containing solutions improve islet yields using standard collagenase digestion techniques. 2. The BSA effect on islet isolation is independent of source and lot of collagenase. 3. The BSA effect on islet isolation is not due solely to its colloid action, as HES failed to achieve the same level of improvement seen with albumin. 4. BSA can protect islets from warm ischemic injury, and the protective action appears to be unique to albumin, as HES was not as effective.
Perdrizet
G A
GA
Department of Surgery, Hartford Hospital, CT 06102-5037, USA.
Rewinski
M J
MJ
Bartus
S A
SA
Hull
D
D
Schweizer
R T
RT
Scharp
D W
DW
D000818:Animals; D002469:Cell Separation / Q000379:methods*; D017364:Collagenases; D003102:Colloids; D005069:Evaluation Studies as Topic; D066298:In Vitro Techniques; D007511:Ischemia / Q000517:prevention & control; D007515:Islets of Langerhans / Q000098:blood supply / Q000166:cytology* / Q000293:injuries; D016381:Islets of Langerhans Transplantation; D007552:Isotonic Solutions; D051381:Rats; D011917:Rats, Inbred Lew; D012710:Serum Albumin, Bovine
D003102:Colloids; C072670:Hanks Balanced Salt Solution; D007552:Isotonic Solutions; D012710:Serum Albumin, Bovine; D017364:Collagenases