Histological examination of the effects of corticosterone in larvae of the western toad, Bufo boreas (Anura: Bufonidae), and the Oriental fire-bellied toad, Bombina orientalis (Anura: Discoglossidae).
The effects of corticosterone (CORT)-treatment on various tissues were examined in two species of anuran larvae, the discoglossid Bombina orientalis, and the bufonid Bufo boreas. Corticosterone was administered directly into aquarium water for 15 days. After treatment, histological analyses were conducted on skin, gut, spleen, thymus, and neural and muscle tissue. Corticosterone treatment prevented sloughing of the skin, which resulted in a build-up of stratum corneum, and inhibited the development of gland nests and the subsequent formation of dermal granular and mucous glands in both species. Corticosterone treatment also decreased epithelial folding in the gut and caused vesiculation of the gut epithelial cells. The thymus of CORT-treated animals was significantly reduced in size (P < .05) and cell density (P < .05), and the spleen of CORT-treated animals was completely involuted. The brain and pituitary of CORT-treated animals had a decreased cell density (P < .05) and many pyknotic cells. An examination of muscle revealed that muscle fibers of CORT-treated animals had a decreased cross-sectional area (P < .05). The dose of CORT used (1.1 microM) was within the range used in other studies in the literature and resulted in tissue levels within the range experienced by larvae at metamorphic climax. Thus, this study is appropriate to address the histological effects of CORT in experimental manipulations and the role of increasing CORT at metamorphic climax. The data suggest that increasing endogenous CORT at metamorphosis may be involved in degeneration of larval tissue, prior to regeneration, which is stimulated by thyroid hormones.
Department of Integrative Biology, University of California, Berkeley 94720, USA.
T B
TB
Hayes
D000818:Animals; D001001:Anura / Q000033:anatomy & histology*; D001921:Brain / Q000166:cytology / Q000187:drug effects; D002025:Bufonidae / Q000033:anatomy & histology*; D002452:Cell Count; D003345:Corticosterone / Q000494:pharmacology*; D007107:Immune System / Q000187:drug effects; D007422:Intestines / Q000187:drug effects; D007814:Larva / Q000187:drug effects*; D008675:Metamorphosis, Biological / Q000187:drug effects; D018482:Muscle, Skeletal / Q000187:drug effects; D010902:Pituitary Gland / Q000166:cytology / Q000187:drug effects; D012867:Skin / Q000187:drug effects
D003345:Corticosterone