The intraductal administration of collagenase in University of Wisconsin solution at the time of harvesting significantly improves porcine islet isolation.
White
S A
SA
Department of Surgery, University of Leicester, UK.
Hughes
D P
DP
Contractor
H H
HH
Johnson
P R
PR
Clayton
H A
HA
Bell
P R
PR
London
N J
NJ
D000241:Adenosine; D000493:Allopurinol; D000818:Animals; D002469:Cell Separation / Q000379:methods*; D003080:Cold Temperature; D017364:Collagenases / Q000008:administration & dosage*; D005069:Evaluation Studies as Topic; D005978:Glutathione; D007328:Insulin; D007515:Islets of Langerhans / Q000166:cytology*; D007552:Isotonic Solutions; D019188:Organ Preservation Solutions; D010183:Pancreatic Ducts; D011887:Raffinose; D013552:Swine; D014021:Tissue Preservation
C072670:Hanks Balanced Salt Solution; D007328:Insulin; D007552:Isotonic Solutions; D019188:Organ Preservation Solutions; C055206:University of Wisconsin-lactobionate solution; D000493:Allopurinol; D017364:Collagenases; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose