Brdu replication bands in the anguilliform fish Echelus myrus.
High-resolution replication banding patterns have been obtained in prometaphase and metaphase chromosomes of the anguilliform fish species Echelus myrus by treating kidney cell cultures with 5-bromodeoxyuridine during the mid-late synthesis phase. The results show the superiority of the in vitro technique in obtaining a higher number of bands which permit an accurate identification of all chromosome pairs. Different replication patterns were compared with C-bands and silver-stained nucleolus organizer regions, providing information on the replication order of different chromatin regions.
Amores
A
A
Departamento de Biologia Celular y Genética, Facultad de Ciencias, Universidad de Malaga, Spain.
Bejar
J
J
Alvarez
M C
MC
D000818:Animals; D001973:Bromodeoxyuridine / Q000378:metabolism*; D002478:Cells, Cultured; D002843:Chromatin / Q000235:genetics / Q000378:metabolism; D002871:Chromosome Banding; D004524:Eels / Q000235:genetics*; D007621:Karyotyping; D007668:Kidney / Q000648:ultrastructure*; D008677:Metaphase / Q000235:genetics; D009697:Nucleolus Organizer Region / Q000235:genetics; D016622:Silver Staining / Q000379:methods
D002843:Chromatin; D001973:Bromodeoxyuridine