Large-scale genomic sequencing: optimization of genomic chemical sequencing reactions.
We have developed a streamlined, reproducible method for performing genomic chemical sequencing reactions on the genomic DNA of Mycoplasma capricolum, which has a genome size of about 750,000 base pairs and whose composition is 75% AT. The general modifications that ensure reproducibility and allow the processing of multiple samples can be widely adopted to other large-scale sequencing projects, while the specific modifications to the chemical reactions are applicable to the sequencing of other DNAs with a high AT content.
Dolan
M
M
Massachusetts General Hospital, Boston, USA.
Ally
A
A
Purzycki
M S
MS
Gilbert
W
W
Gillevet
P M
PM
D000225:Adenine / Q000737:chemistry; D001482:Base Composition; D016386:Chromosome Walking; D015342:DNA Probes; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification*; D004586:Electrophoresis / Q000379:methods; D016680:Genome, Bacterial; D009174:Mycoplasma / Q000235:genetics*; D015203:Reproducibility of Results; D017422:Sequence Analysis, DNA / Q000379:methods*; D013941:Thymine / Q000737:chemistry
D015342:DNA Probes; D004269:DNA, Bacterial; D000225:Adenine; D013941:Thymine