Rehybridization on metaphases studied previously by FISH. An approach to analyze chromosome aberrations.
A new protocol for fluorescence in situ rehybridization is described. Biotin-labeled chromosome-specific DNA probes were hybridized onto metaphases which previously had been studied by FISH. This method makes it possible to reexamine the same metaphase spreads with different DNA probes. It allows for precise characterization of cytogenetic translocations and for detection of multiple aberrations presented in a karyotype. It is especially useful in cases where a limited number of cytogenetic preparations are available.
National Laboratory of Molecular Oncology, Chinese Academy of Medical Sciences, Chaoyang Qu, Beijing, P.R. China.
M R
MR
Wang
B
B
Perissel
P
P
Malet
D002869:Chromosome Aberrations; D015342:DNA Probes; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D007621:Karyotyping; D008677:Metaphase; D014407:Tumor Cells, Cultured; D001749:Urinary Bladder Neoplasms / Q000235:genetics*
D015342:DNA Probes