A differentiated silver intensification procedure for the peroxidase-diaminobenzidine reaction.
Several silver-based procedures have been developed to intensify the peroxidase-catalyzed diaminobenzidine polymer used as a marker in immunohistochemistry. Many of these methods use acutely unstable reagents or reagents that are not amenable to batch processing. We describe a new procedure with an initial treatment in dilute ammoniacal silver, followed by a differentiation step to remove background staining and a final toning in 0.2% gold chloride. The procedure provides a jet-black intensification of the tan diaminobenzidine product, is amenable to batch processing of free-floating sections and slide-mounted sections, and does not require acid-cleaned glassware.
Department of Brain & Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, USA.
B
B
Quinn
A M
AM
Graybiel
D015100:3,3'-Diaminobenzidine; D000818:Animals; D001923:Brain Chemistry; D007150:Immunohistochemistry; D009195:Peroxidase; D011506:Proteins / Q000032:analysis; D051381:Rats; D016622:Silver Staining / Q000379:methods*; D013194:Staining and Labeling
D011506:Proteins; D015100:3,3'-Diaminobenzidine; D009195:Peroxidase