Immunohistochemical assessment of cell proliferation in plant tissues using formaldehyde-fixed paraffin-embedded material.
Cell proliferation in formaldehyde-fixed and wax-embedded sections of root tissues from Vicia faba beans was assessed by using a monoclonal antibody raised against the proliferating cell nuclear antigen (PCNA). Positive PCNA immunoreactivity was restricted to nuclei in known proliferating zones of the root. The immunoreactivity of cells in metaphase/anaphase of mitosis showed weaker PCNA immunoreactivity while nuclei in telophase showed no PCNA immunoreactivity indicating that this method is well suited to label proliferating cells in plant tissues.
University of Southampton, UK.
U
U
Schumacher
D000911:Antibodies, Monoclonal; D002455:Cell Division / Q000502:physiology; D002467:Cell Nucleus / Q000737:chemistry; D007887:Fabaceae / Q000737:chemistry; D005557:Formaldehyde; D007150:Immunohistochemistry; D016612:Paraffin Embedding; D018517:Plant Roots / Q000737:chemistry / Q000166:cytology*; D010946:Plants, Medicinal; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D016707:Tissue Fixation
D000911:Antibodies, Monoclonal; D018809:Proliferating Cell Nuclear Antigen; D005557:Formaldehyde