Ethylene-binding sites generated in yeast expressing the Arabidopsis ETR1 gene.
Mutations in the ETR1 gene of Arabidopsis thaliana confer insensitivity to ethylene, which indicates a role for the gene product in ethylene signal transduction. Saturable binding sites for [14C]ethylene were detected in transgenic yeast expressing the ETR1 protein, whereas control yeast lacking ETR1 showed no detectable ethylene binding. Yeast expressing a mutant form of ETR1 (etr1-1) also showed no detectable ethylene binding, which provides an explanation for the ethylene-insensitive phenotype observed in plants carrying this mutation. Expression of truncated forms of ETR1 in yeast provided evidence that the amino-terminal hydrophobic domain of the protein is the site of ethylene binding. It was concluded from these results that ETR1 acts as an ethylene receptor in Arabidopsis.
Schaller
G E
GE
Department of Botany, University of Wisconsin, Madison 53706, USA.
Bleecker
A B
AB
D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D001665:Binding Sites; D003001:Cloning, Molecular; D005030:Ethylenes / Q000378:metabolism*; D017343:Genes, Plant; D016297:Mutagenesis, Site-Directed; D010446:Peptide Fragments / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae
D005030:Ethylenes; D010446:Peptide Fragments; D010940:Plant Proteins; D011956:Receptors, Cell Surface; D011994:Recombinant Proteins; C083750:ethylene receptors, plant; C036216:ethylene