An antigen detection immunoassay for big liver and spleen disease agent.
An enzyme-linked immunosorbent assay (ELISA) able to detect an antigen associated with infection with big liver and spleen (BLS) agent was developed. The assay is a capture ELISA (C-ELISA) procedure using ELISA plates coated with mouse monoclonal antibody (mAb 1H4) for antigen capture and a polyclonal chicken Ig preparation, extracted from yolk of BLS antibody positive birds, for detection of captured BLS antigen. mAb 1H4 was produced to a partially purified antigen preparation from liver suspensions of naturally BLS infected birds. The C-ELISA was evaluated for specificity and sensitivity in naturally and experimentally BLS infected and uninfected broiler and layer breeds of domestic fowl.
Ellis
T M
TM
Animal Industries Division, Department of Agriculture, South Perth, WA, Australia.
Payne
C J
CJ
Plant
S L
SL
Gregory
A R
AR
D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D000956:Antigens, Viral / Q000032:analysis*; D002645:Chickens; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary; D005260:Female; D006529:Hepatomegaly / Q000662:veterinary* / Q000821:virology; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology; D011201:Poultry Diseases / Q000821:virology*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013163:Splenomegaly / Q000662:veterinary* / Q000821:virology; D014777:Virus Diseases / Q000175:diagnosis / Q000662:veterinary*
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral