Effects of concanavalin A and pokeweed lectins on microvillar membrane proteins during the organ culture of rabbit intestinal mucosa.
The effects of pokeweed lectin (PWL) and concanavalin A (Con A) on microvillar membrane (MVM) proteins during the organ culture of rabbit ileal explants for 24 hours were compared with the known effects of enteropathogenic Escherichia coli (EPEC). PWL resulted in the accelerated release of brush border enzymes into the culture medium, accompanied by decreased tissue activities and an increase in the total activity present in the tissue and culture medium. Con A had less effect on the release and tissue activities of brush border enzymes and the total activity was not increased. Sucrose density gradient centrifugation of the culture medium showed that MVM enzymes were predominantly particulate, consistent with their release as vesicles. Centrifugation of ileal explants showed that PWL, but not Con A, resulted in a decrease in the modal density of the brush border which was consistent with a lower glycoprotein-to-lipid ratio. These findings suggest that PWL, in common with EPEC, may cause the disruption and vesiculation of microvilli and the compensatory stimulation of MVM protein synthesis.
Department of Small Animal Medicine, Royal Veterinary College, University of London, North Mymms, Hertfordshire.
H
H
Embaye
L
L
Whybrew
R M
RM
Odedra
C A
CA
Hart
J N
JN
Fletcher
J R
JR
Saunders
R M
RM
Batt
D000818:Animals; D003208:Concanavalin A / Q000494:pharmacology*; D004927:Escherichia coli Infections / Q000378:metabolism / Q000473:pathology; D007082:Ileum / Q000187:drug effects / Q000378:metabolism; D007413:Intestinal Mucosa / Q000187:drug effects* / Q000378:metabolism; D008565:Membrane Proteins / Q000187:drug effects* / Q000378:metabolism; D008871:Microvilli / Q000187:drug effects* / Q000378:metabolism; D009924:Organ Culture Techniques; D011043:Pokeweed Mitogens / Q000494:pharmacology*; D011817:Rabbits
D008565:Membrane Proteins; D011043:Pokeweed Mitogens; D003208:Concanavalin A