Viral RNA as a potential target for two independent mechanisms of replicase-mediated resistance against cucumber mosaic virus.
Transgenic tobacco showing replicase-mediated resistance against cucumber mosaic virus (CMV) can be infected by the strain K-CMV. By use of chimeric constructs between full-length cDNA clones of RNA2 of strains Fny-CMV and K-CMV, the existence of two independent mechanisms of replicase-mediated resistance against viral replication and movement of Fny-CMV was demonstrated in these plants. The data indicate that viral RNA may serve as the target for both mechanisms of resistance. A positive correlation was observed between the amount of K-CMV RNA2 sequence present in the chimeric constructs and the ability to overcome the inhibition of replication, whereas a sequence domain was delimited in K-CMV RNA2 responsible for the ability of this strain to break resistance against virus movement.
Department of Plant Pathology, Cornell University, Ithaca, New York 14853, USA.
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Hellwald
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Palukaitis
D001483:Base Sequence; D017799:Cucumovirus / Q000235:genetics / Q000472:pathogenicity / Q000502:physiology*; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000821:virology*; D018515:Plant Leaves / Q000821:virology; D030821:Plants, Genetically Modified; D010947:Plants, Toxic; D011523:Protoplasts / Q000821:virology; D012313:RNA; D012367:RNA, Viral / Q000032:analysis / Q000235:genetics* / Q000502:physiology; D012324:RNA-Dependent RNA Polymerase / Q000378:metabolism / Q000502:physiology*; D014026:Nicotiana / Q000235:genetics / Q000821:virology; D014764:Viral Proteins / Q000032:analysis; D014779:Virus Replication
D012367:RNA, Viral; C039919:RNA, recombinant; D014764:Viral Proteins; D012313:RNA; D012324:RNA-Dependent RNA Polymerase