Site-directed mutagenesis of HIV-1 protease: generation of mutant proteases with increased stability to autodigestion.
Tomasselli
A G
AG
Upjohn Laboratories, Upjohn Company, Kalamazoo, Michigan 49001, USA.
Mildner
A M
AM
Rothrock
D J
DJ
Sarcich
J L
JL
Lull
J
J
Leone
J
J
Heinrikson
R
R
D000595:Amino Acid Sequence; D002384:Catalysis; D003001:Cloning, Molecular / Q000379:methods; D004795:Enzyme Stability; D004926:Escherichia coli; D016333:HIV Protease / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D015497:HIV-1 / Q000201:enzymology*; D007700:Kinetics; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009842:Oligopeptides / Q000737:chemistry / Q000378:metabolism; D010446:Peptide Fragments / Q000737:chemistry / Q000302:isolation & purification; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D015183:Restriction Mapping; D013379:Substrate Specificity
D009842:Oligopeptides; D010446:Peptide Fragments; D011994:Recombinant Proteins; D016333:HIV Protease