Oxidized low-density lipoprotein induces the production of superoxide by neutrophils.
Exposure of guinea pig peritoneal neutrophils to ox-LDL led to the production of superoxide, which was measured by the formation of superoxide-dependent chemiluminescence. The cells exposed to unoxidized LDL, e.g. native LDL, acetyl-LDL, and self-aggregates of LDL showed no production of superoxide. The superoxide production was correlated with the levels of oxidative modification of LDL and reached a maximum between 10 and 30 min during incubation, but preincubating the cells with cytochalasin B decreased the superoxide production. These findings indicate that neutrophils rapidly take up ox-LDL by phagocytosis and generate superoxide which may cause superoxide-mediated lipid peroxidation in vivo.
Department of Biochemistry, Teikyo University School of Medicine, Tokyo, Japan.
R
R
Maeba
A
A
Maruyama
O
O
Tarutani
N
N
Ueta
H
H
Shimasaki
D000818:Animals; D003571:Cytochalasin B / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D006168:Guinea Pigs; D008077:Lipoproteins, LDL / Q000494:pharmacology*; D008163:Luminescent Measurements; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D009895:Opsonin Proteins / Q000494:pharmacology; D010084:Oxidation-Reduction; D010529:Peritoneal Cavity / Q000166:cytology; D013481:Superoxides / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D015054:Zymosan / Q000494:pharmacology
D008077:Lipoproteins, LDL; D009895:Opsonin Proteins; D013481:Superoxides; D003571:Cytochalasin B; D015054:Zymosan; D013755:Tetradecanoylphorbol Acetate