Three-dimensional reconstruction of the feline larynx with serial histologic sections.
This paper reviews a new technique to develop high-resolution three-dimensional (3-D) images of the larynx using histological sections. Three-dimensional computer-reconstructed histological sections of the cat are used in this study to evaluate the recurrent laryngeal nerve (RLN) in its true anatomic course, with emphasis on its relationship to surrounding structures (laryngeal framework). A cat model was used because of specimen availability and technical ease of tissue preparation. Computer-reconstructed serial histologic sections add a new dimension to the study of laryngeal anatomy, laryngeal trauma, and growth patterns of laryngeal cancer. The technical aspects of three-dimensional reconstruction and future applications are discussed.
Department of Otolaryngology-Head and Neck Surgery, Johns Hopkins University, Baltimore, Md., USA.
R L
RL
Moses
P W
PW
Flint
C H
CH
Paik
S J
SJ
Zinreich
C W
CW
Cummings
D000818:Animals; D002415:Cats; D006652:Histological Techniques; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D007830:Larynx / Q000002:abnormalities* / Q000166:cytology