Devazepide reverses the anorexic effect of simmondsin in the rat.
Simmondsin, a glycoside extracted from jojoba meal (Simmondsia chinensis), causes a reduction in food intake after oral administration. To investigate the mechanism by which simmondsin reduces food intake, fasted and free-feeding rats were given simmondsin-supplemented food and simultaneously injected with devazepide, a specific antagonist of peripheral-type cholecystokinin receptors (CCKA receptors). In free-feeding rats, supplementation of food with 0.5% simmondsin caused a reduction in food intake of +/- 40% in the period of 4 h following food presentation. Intraperitoneal injection of 100 micrograms devazepide/kg body weight prevented this effect. In rats fasted for 20 h, the food intake in the 30 min after presentation of food supplemented with 0.15% or 0.50% simmondsin was reduced in a dose-related manner; this was also inhibited by simultaneous application of devazepide. It is suggested that peripheral CCKA receptors are involved in the effect of simmondsin on food intake. However, a direct effect of simmondsin on CCKA receptors has been excluded, since simmondsin was unable to cause contraction of the guinea-pig gallbladder in vitro.
Interdisciplinary Research Centre, Katholieke Universiteit Leuven Campus Kortrijk, Universitaire Campus, Belgium.
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MM
Cokelaere
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Busselen
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G
Flo
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P
Daenens
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Decuypere
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Kühn
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Van Boven
D000097:Acetonitriles / Q000494:pharmacology*; D000818:Animals; D001067:Appetite Depressants / Q000494:pharmacology*; D001570:Benzodiazepinones / Q000494:pharmacology*; D003510:Cyclohexanes; D020109:Devazepide; D004305:Dose-Response Relationship, Drug; D004435:Eating / Q000187:drug effects*; D005960:Glucosides / Q000494:pharmacology*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D011949:Receptors, Cholecystokinin / Q000037:antagonists & inhibitors*
D000097:Acetonitriles; D001067:Appetite Depressants; D001570:Benzodiazepinones; D003510:Cyclohexanes; D005960:Glucosides; D011949:Receptors, Cholecystokinin; D020109:Devazepide; C089377:simmondsin