Structural organization and genetic localization of the human bone morphogenetic protein 1/mammalian tolloid gene.
Bone morphogenetic protein 1 (BMP1) is a putative protease purified from extracts capable of inducing ectopic bone formation. A single mammalian gene apparently encodes alternatively spliced transcripts for BMP1, for a longer protein with a domain structure identical to that of the Drosophila dorsal-ventral patterning gene product tolloid (Tld), and for a third species of low abundance. Here we describe the organization of the 46-kb, 22-exon human BMP1/mTld gene that encodes these forms. Exons corresponding to each of the alternatively spliced transcripts are identified, and comparison with the Drosophila Tld gene reveals alignment of introns at only three positions. The major BMP1/mTld transcription start site is found only 706 bp downstream of the polyadenylation site of the SFTP2 surfactant gene, and a previously reported highly polymorphic CA repeat is found within the BMP1/mTld first intron. These two findings place the BMP1/mTld gene between markers D8S298 and D8S5 on the genetic map.
Takahara
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Department of Pathology and Laboratory Medicine, University of Wisconsin, Madison 53706, USA.
Lee
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Wood
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Greenspan
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D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D019485:Bone Morphogenetic Proteins; D002874:Chromosome Mapping; D017931:DNA Primers; D004330:Drosophila / Q000235:genetics; D029721:Drosophila Proteins; D005091:Exons; D005260:Female; D015186:Hominidae / Q000235:genetics*; D006801:Humans; D007301:Insect Hormones / Q000235:genetics; D007438:Introns; D008322:Mammals; D008562:Membrane Glycoproteins / Q000235:genetics; D008969:Molecular Sequence Data; D010920:Placenta / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D011247:Pregnancy; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics*; D011663:Pulmonary Surfactants / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D011956:Receptors, Cell Surface; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D051193:Toll-Like Receptors
D019485:Bone Morphogenetic Proteins; D017931:DNA Primers; D029721:Drosophila Proteins; D007301:Insect Hormones; D008562:Membrane Glycoproteins; D011506:Proteins; D011663:Pulmonary Surfactants; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; C068721:Tl protein, Drosophila; D051193:Toll-Like Receptors