The use of natural and unnatural amino acid substrates to define the substrate specificity differences of Escherichia coli aspartate and tyrosine aminotransferases.
The tyrosine (eTATase) and aspartate (eAATase) aminotransferases of Escherichia coli transaminate diacarboxylic amino acids with similar rate constants. However, eTATase exhibits approximately 10(2)-10(4)-fold higher second-order rate constants for the transamination of aromatic amino acids than does eAATase. A series of natural and unnatural amino acid substrates was used to quantitate specificity differences for these two highly related enzymes. A general trend toward lower transamination activity with increasing side-chain length (extending from aspartate to glutamate to alpha-aminoadipate) is observed for both enzymes. This result suggests that dicarboxylate ligands associate with the two highly related enzymes in a similar manner. The high reactivity of the enzymes with L-Asp and L-Glu can be attributed to an ion pair interaction between the side-chain carboxylate of the amino acid substrate and the guanidino group of the active site residue Arg 292 that is common to both enzymes. A strong linear correlation between side-chain hydrophobicity and transamination rate constants obtains for n-alkyl side-chain amino substrates with eTATase, but not for eAATase. The present kinetic data support a model in which eAATase contains one binding mode for all classes of substrate, whereas the active site of eTATase allows an additional mode that has increased affinity for hydrophobic amino acid.
Department of Molecular and Cell Biology, University of California, Berkeley 94720, USA.
J J
JJ
Onuffer
B T
BT
Ton
I
I
Klement
J F
JF
Kirsch
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000378:metabolism*; D001120:Arginine / Q000378:metabolism; D001219:Aspartate Aminotransferases / Q000235:genetics / Q000378:metabolism*; D001224:Aspartic Acid / Q000378:metabolism; D001665:Binding Sites; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D018698:Glutamic Acid / Q000378:metabolism; D007700:Kinetics; D008969:Molecular Sequence Data; D013379:Substrate Specificity; D014316:Tritium; D014444:Tyrosine Transaminase / Q000235:genetics / Q000378:metabolism*
D000596:Amino Acids; D014316:Tritium; D001224:Aspartic Acid; D018698:Glutamic Acid; D001120:Arginine; D001219:Aspartate Aminotransferases; D014444:Tyrosine Transaminase