Differentiation of Serratia liquefaciens into swarm cells is controlled by the expression of the flhD master operon.
The velocity with which a swarming colony of Serratia liquefaciens colonizes the surface of a suitable solid substratum was controlled by modulating the expression of the flhD master operon. In liquid medium, the stimulation of flhD expression resulted in filamentous, multinucleate, and hyperflagellated cells that were indistinguishable from swarm cells isolated from the edge of a swarm colony. Thus, expression of the flhD master operon appears to play a central role in the process of swarm cell differentiation.
Eberl
L
L
Department of Microbiology, Technical University of Denmark, Lyngby, Denmark.
Christiansen
G
G
Molin
S
S
Givskov
M
M
D001426:Bacterial Proteins / Q000235:genetics*; D003470:Culture Media; D004268:DNA-Binding Proteins / Q000235:genetics*; D004790:Enzyme Induction / Q000187:drug effects; D029968:Escherichia coli Proteins; D005407:Flagella / Q000502:physiology; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D007544:Isopropyl Thiogalactoside / Q000494:pharmacology; D009876:Operon / Q000235:genetics*; D010741:Phospholipases A / Q000378:metabolism; D012705:Serratia / Q000201:enzymology / Q000235:genetics / Q000502:physiology*; D015534:Trans-Activators / Q000235:genetics*; D001616:beta-Galactosidase / Q000096:biosynthesis / Q000235:genetics
D001426:Bacterial Proteins; D003470:Culture Media; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; D015534:Trans-Activators; C090553:flhD protein, E coli; D007544:Isopropyl Thiogalactoside; D010741:Phospholipases A; D001616:beta-Galactosidase