Immunohistochemical localization of fibroblast growth factor-1 (FGF-1) and FGF-2 in cultured human ameloblastoma epithelial cells and ameloblastoma tissues.
Fibroblast growth factor-1 (FGF-1) and FGF-2 are mitogenic polypeptides that may contribute to neoplastic cell proliferation. In the present study, we established a serum-free culture system for ameloblastoma cells and demonstrated that the addition of FGF-1 and FGF-2 enhanced cell growth in a dose-dependent manner. Immunoperoxidase staining of cultured cells demonstrated strong expression of FGF-1 and FGF-2. In tissue specimens, FGF-1 was localized in epithelial cell components of ameloblastomas, whereas FGF-2 was mainly found in the basement membranes with only moderate staining in epithelium. These data suggest that both FGF-1 and FGF-2 may contribute to the growth and development of ameloblastomas.
Department of Oral & Maxillofacial Surgery I, School of Dentistry, Hiroshima University, Japan.
Y
Y
Myoken
Y
Y
Myoken
T
T
Okamoto
J D
JD
Sato
K
K
Takada
D000293:Adolescent; D000328:Adult; D000564:Ameloblastoma / Q000378:metabolism / Q000473:pathology*; D000911:Antibodies, Monoclonal; D001485:Basement Membrane / Q000378:metabolism / Q000473:pathology; D002455:Cell Division / Q000187:drug effects; D002648:Child; D016895:Culture Media, Serum-Free; D004305:Dose-Response Relationship, Drug; D004848:Epithelium / Q000378:metabolism / Q000473:pathology; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000008:administration & dosage / Q000032:analysis* / Q000494:pharmacology; D005346:Fibroblast Growth Factors / Q000008:administration & dosage / Q000032:analysis* / Q000494:pharmacology; D006801:Humans; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D008297:Male; D013194:Staining and Labeling; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D016895:Culture Media, Serum-Free; D016222:Fibroblast Growth Factor 2; D005346:Fibroblast Growth Factors