Phage display: protein engineering by directed evolution.
Phage display of proteins has become an important tool for protein engineering. Over the past year, the versatility of the technology has expanded to include the development of DNA-binding proteins with novel specificities, energetics of protein folding and directed evolution of antibodies. In addition, display of expressed cDNA libraries opens an exciting opportunity for studying protein-protein interactions.
O'Neil
K T
KT
DuPont Merck Pharmaceutical Company, Chemical and Physical Sciences Department, Wilmington, DE 19880-0328, USA.
Hoess
R H
RH
D000906:Antibodies / Q000235:genetics; D001435:Bacteriophages / Q000235:genetics*; D002384:Catalysis; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D019020:Directed Molecular Evolution; D004798:Enzymes / Q000235:genetics; D006801:Humans; D015202:Protein Engineering; D017510:Protein Folding; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
D000906:Antibodies; D004268:DNA-Binding Proteins; D004798:Enzymes; D011994:Recombinant Proteins