Vaccination protects against in vivo-grown feline immunodeficiency virus even in the absence of detectable neutralizing antibodies.
So far, vaccination experiments against feline immunodeficiency virus have used in vitro-grown virus to challenge the vaccinated hosts. In this study, cats were vaccinated with fixed feline immunodeficiency virus-infected cells and challenged with plasma obtained from cats infected with the homologous virus diluted to contain 10 cat 50% infectious doses. As judged by virus culture, PCRs, and serological analyses performed over an 18-month period after the challenge, all of the vaccinated cats were clearly protected. Interestingly, prior to challenge most vaccines lacked antibodies capable of neutralizing a fresh isolate of the homologous virus.
Department of Biomedicine, University of Pisa, Italy.
D
D
Matteucci
M
M
Pistello
P
P
Mazzetti
S
S
Giannecchini
D
D
Del Mauro
L
L
Zaccaro
P
P
Bandecchi
F
F
Tozzini
M
M
Bendinelli
D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood* / Q000276:immunology; D001483:Base Sequence; D002415:Cats; D002460:Cell Line; D017931:DNA Primers; D016181:Feline Acquired Immunodeficiency Syndrome / Q000517:prevention & control*; D005260:Female; D016089:Immunodeficiency Virus, Feline / Q000276:immunology* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D012091:Repetitive Sequences, Nucleic Acid; D013601:T-Lymphocytes / Q000276:immunology; D014765:Viral Vaccines / Q000008:administration & dosage*
D000914:Antibodies, Viral; D017931:DNA Primers; D014765:Viral Vaccines