Ataxia and epileptic seizures in mice lacking type 1 inositol 1,4,5-trisphosphate receptor.
The inositol 1,4,5-trisphosphate (InsP3) receptor acts as an InsP3-gated Ca2+ release channel in a variety of cell types. Type 1 InsP3 receptor (IP3R1) is the major neuronal member of the IP3R family in the central nervous system, predominantly enriched in cerebellar Purkinje cells but also concentrated in neurons in the hippocampal CA1 region, caudate-putamen, and cerebral cortex. Here we report that most IP3R1-deficient mice generated by gene targeting die in utero, and born animals have severe ataxia and tonic or tonic-clonic seizures and die by the weaning period. An electroencephalogram showed that they suffer from epilepsy, indicating that IP3R1 is essential for proper brain function. However, observation by light microscope of the haematoxylin-eosin staining of the brain and peripheral tissues of IP3R1-deficient mice showed no abnormality, and the unique electrophysiological properties of the cerebellar Purkinje cells of IP3R1-deficient mice were not severely impaired.
Matsumoto
M
M
Department of Molecular Neurobiology, University of Tokyo, Japan.
Nakagawa
T
T
Inoue
T
T
Nagata
E
E
Tanaka
K
K
Takano
H
H
Minowa
O
O
Kuno
J
J
Sakakibara
S
S
Yamada
M
M
Yoneshima
H
H
Miyawaki
A
A
Fukuuchi
Y
Y
Furuichi
T
T
Okano
H
H
Mikoshiba
K
K
Noda
T
T
D000818:Animals; D001259:Ataxia / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D001921:Brain / Q000378:metabolism; D015220:Calcium Channels / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D017931:DNA Primers; D004569:Electroencephalography; D004827:Epilepsy / Q000235:genetics / Q000378:metabolism*; D005313:Fetal Death; D018390:Gene Targeting; D015544:Inositol 1,4,5-Trisphosphate / Q000378:metabolism*; D053496:Inositol 1,4,5-Trisphosphate Receptors; D051379:Mice; D008969:Molecular Sequence Data; D009154:Mutation; D011689:Purkinje Cells / Q000378:metabolism; D018160:Receptors, Cytoplasmic and Nuclear / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*
D015220:Calcium Channels; D017931:DNA Primers; D053496:Inositol 1,4,5-Trisphosphate Receptors; D018160:Receptors, Cytoplasmic and Nuclear; D015544:Inositol 1,4,5-Trisphosphate