Isoenzyme profile of Paracoccidioides brasiliensis.
Isoenzyme profiles of 10 strains of Paracoccidioides brasiliensis from different origins (nine strains from patients with different clinical forms of paracoccidioidomycosis and one from the faeces of a penguin) were determined by polyacrylamide gel electrophoresis using 37 different enzymes. Differences in carbonate dehydratase, phosphoglucomutase, phosphoglucoseisomerase, glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase, lactate dehydrogenase and beta-esterase were detected among the isolates studied allowing the characterization of nine zymodemes. Two isolates showed identical profiles. There was no correlation between the zymodeme patterns and virulence, clinical forms of the disease nor age of the cultures.
Svidzinsky
T I
TI
Universidade Federal de São Paulo, Escola Paulista de Medicina, Disciplina de Biologia Celular, Brazil.
Camargo
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ZP
D006801:Humans; D007527:Isoenzymes / Q000302:isolation & purification*; D010088:Oxidoreductases / Q000302:isolation & purification; D010228:Paracoccidioides / Q000201:enzymology*; D010229:Paracoccidioidomycosis / Q000382:microbiology*
D007527:Isoenzymes; D010088:Oxidoreductases