Perfusion of gills isolated from the hyper-hyporegulating crab Pachygrapsus marmoratus (Crustacea, Decapoda): adaptation of a method.
This paper reports the adaptation of a branchial perfusion technique to the gills of the small hyper-hyporegulating crab, Pachygrapsus marmoratus. The physiological quality of the preparation has been established and preliminary measurements of the transepithelial potential difference (PD) and sodium fluxes were reported. A clear-cut distinction has to be made between anterior and posterior gills. With symmetrical bathing conditions (same saline on both sides of the epithelium), a significant transepithelial PD was measured only in posterior gills isolated from crabs acclimated to dilute sea water. This hemolymph-side negative PD is shown to require biological energy and to be sensitive to ouabain. Na+ influx was larger than efflux, indicating the occurrence of a net inward of Na+. The active nature of the Na+ influx was established.
Pierrot
C
C
Laboratoire d'Ecophysiologie des Invertébrés, Université Montpellier II, France.
Pequeux
A
A
Thuet
P
P
D000818:Animals; D003386:Brachyura / Q000502:physiology*; D004848:Epithelium / Q000187:drug effects; D005260:Female; D005880:Gills / Q000502:physiology; D006458:Hemolymph / Q000378:metabolism; D066298:In Vitro Techniques; D017136:Ion Transport / Q000502:physiology; D008297:Male; D010100:Oxygen / Q000494:pharmacology; D010477:Perfusion / Q000379:methods*; D011199:Potentiometry; D012964:Sodium / Q000378:metabolism; D014882:Water-Electrolyte Balance / Q000502:physiology
D012964:Sodium; D010100:Oxygen