MSG gene cluster encoding major cell surface glycoproteins of rat Pneumocystis carinii.
The MSG genes of Pneumocystis carinii encode major cell surface glycoproteins of multi-gene families and play crucial roles in the pathobiology of P. carinii pneumocystosis. The 11,370-bp chromosomal DNA encoding MSG was cloned and sequenced. It contained three open reading frames in tandem repeat, MSG99, MSG100 and ORF-3. MSG99 and MSG100 shared 39% protein sequence identity and belonged to the same MSG family, regardless of the internal ca. 1,000-bp deletion in MSG99. ORF-3 encodes a putative 47,820-Da protein and had no sequence similarities to MSG molecules or any known proteins. However, the ORF-3 protein was rich in proline residues and highly hydrophilic except for the C-terminal region, which seemed to be an anchoring sequence in the cell membrane. Northern and Southern blot hybridization analyses revealed a 3-kb ORF-3 transcript as well as multiple DNA fragments carrying the MSG and ORF-3 sequences. These results strongly suggested that diverse MSG genes are clustered in tandem repeats and these clusters involve ORF-3. The possible association of ORF-3 protein in the cell membrane is discussed.
Wada
M
M
Department of Tumor Biology, University of Tokyo, Japan.
Nakamura
Y
Y
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics*; D005800:Genes, Fungal; D008562:Membrane Glycoproteins / Q000235:genetics*; D008969:Molecular Sequence Data; D005810:Multigene Family; D016366:Open Reading Frames; D011010:Pneumocystis / Q000235:genetics*; D051381:Rats
D004271:DNA, Fungal; D005656:Fungal Proteins; D008562:Membrane Glycoproteins; C077556:GPA protein, Pneumocystis carinii sp.