The entry of reovirus into L cells is dependent on vacuolar proton-ATPase activity.
Inhibitors of vacuolar proton-ATPase activity (5 microM bafilomycin A1 or 50 nM concanamycin A) prevented infection by reovirus particles but not by infectious subviral particles (ISVPs). Neither compound affected virus attachment or internalization. However, both compounds potently blocked cleavage of the viral protein mu 1C. Finally, both reovirus particles and ISVPs efficiently translocated the toxin alpha-sarcin to the cytosol during virus entry. Bafilomycin A1 blocked translocation of alpha-sarcin by reovirus particles but not by ISVPs.
Departamento de Bioquímica y Biología Molecular, Facultad de Farmacia, Universidad de Santiago de Compostela, La Coruña, Spain.
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CG
Martínez
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R
Guinea
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J
Benavente
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L
Carrasco
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002213:Capsid / Q000378:metabolism; D036022:Capsid Proteins; D002463:Cell Membrane Permeability; D004722:Endoribonucleases; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005656:Fungal Proteins / Q000378:metabolism; D007739:L Cells; D018942:Macrolides; D051379:Mice; D006180:Proton-Translocating ATPases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D012087:Reoviridae / Q000472:pathogenicity / Q000502:physiology*; D014617:Vacuoles / Q000201:enzymology
D000900:Anti-Bacterial Agents; D036022:Capsid Proteins; D004791:Enzyme Inhibitors; D005656:Fungal Proteins; D018942:Macrolides; C055356:mu1 protein, Reovirus; C014801:alpha-sarcin; C036978:concanamycin A; C040929:bafilomycin A1; D004722:Endoribonucleases; D006180:Proton-Translocating ATPases