[Paternity study applying DNA polymorphism: evaluation of methods traditionally used in Chile].
Simultaneous detection of several VNTR loci using a single DNA probe is the basis of the technique called "DNA fingerprint" (DNAfp) of increasing application in parenthood identification. According to the data gathered by different laboratories worldwide, father exclusion can be made in a larger number of cases when compared with the customary tests based on erythrocyte antigens. The question could then be whether DNAfp will completely replace erythrocyte antigens tests. We report here our experience in applying DNAfp to 92 samples corresponding to 34 paternity cases and comparing these with the results obtained with the antigens of the systems ABO, Rh, MNSs, Duffy and Kidd. Most of the HaeIII/digested DNA samples produced 13 to 16 bands larger than 4.3 Kb (average 14,0761 +/- 2,205). Average band sharing between pairs of unrelated individual was 1,9107 +/- 1,083. Two cases presenting an a posteriori probability of being the father of 80.7% and 76.5% by erythrocyte antigens were clearly excluded by DNAfp. All exclusions made by antigens were confirmed by DNAfp. In the cases reported as father "rather probable" (28 cases) by DNAfp, these shared with the child 6,7407 +/- 1.7 bands on average. Because of time, cost and simplicity we favor a procedure starting with the antigens test and continuing with DNAfp only when an exclusion is not possible. Economy will increase as the number of exclusions increases.
Departamento de Medicina, Facultad de Medicina, Universidad de Chile, Santiago de Chile.
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Armanet
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Aguirre
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Vargas
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Llop
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Castillo
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Cifuentes
D001788:Blood Grouping and Crossmatching; D002677:Chile; D016172:DNA Fingerprinting / Q000379:methods*; D015342:DNA Probes / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D005819:Genetic Markers; D006801:Humans; D008297:Male; D010334:Paternity; D011110:Polymorphism, Genetic / Q000235:genetics*
D015342:DNA Probes; D005819:Genetic Markers
Estudio de paternidad aplicando el polimorfismo de DNA: Evaluación en relación a los métodos convencionalmente usados en Chile.