Degradation of chlorobiphenyls catalyzed by the bph-encoded biphenyl-2,3-dioxygenase and biphenyl-2,3-dihydrodiol-2,3-dehydrogenase of Pseudomonas sp. LB400.
In order to characterize the metabolites produced in vivo by biphenyl-2,3-dioxygenase and biphenyl-2,3-dihydrodiol-2,3-dehydrogenase, the first two enzymes of the (polychloro)biphenyl catabolic pathway encoded by the bph locus of Pseudomonas sp. LB400, recombinant E. coli strains expressing the respective genes were constructed. Biphenyl-2,3-dioxygenase attack on 2,2'- or 2,4'-dichlorobiphenyl was shown to give rise to virtually quantitative ortho-dechlorination of these congeners by hydroxylation at the chlorinated carbon 2 and its unsubstituted neighbour. Elimination of hydrochloric acid directly leads to 2,3-dihydroxy-chlorobiphenyls and obviates the need for biphenyl-2,3-dihydrodiol-2,3-dehydrogenase for the catabolism of such congeners.
Seeger
M
M
Division of Microbiology, Gesellschaft für Biotechnologische Forschung, National Research Centre for Biotechnology, Braunschweig, Germany.
Timmis
K N
KN
Hofer
B
B
D001713:Biphenyl Compounds / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D007506:Iron-Sulfur Proteins; D010088:Oxidoreductases / Q000378:metabolism*; D010105:Oxygenases / Q000235:genetics / Q000378:metabolism*; D011549:Pseudomonas / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
D001713:Biphenyl Compounds; D007506:Iron-Sulfur Proteins; D011994:Recombinant Proteins; D010088:Oxidoreductases; D010105:Oxygenases; C074161:biphenyl-2,3-dioxygenase; C048338:trans-1,2-dihydrobenzene-1,2-diol dehydrogenase