Further study on the use of uncharged beta-cyclodextrin polymer in capillary electrophoresis: enantiomeric separation of some alpha-hydroxy acids.
Uncharged beta-cyclodextrin polymer was used as chiral selector for the enantiomeric separation of some alpha-hydroxy acids by capillary electrophoresis. Complexation and enantiomeric resolution of mandelic acid, m-hydroxy and p-hydroxymandelic acid, 3,4-dihydroxymandelic acid, as well as 2- and 3-phenyllactic acid were studied, changing the concentration of the beta-cyclodextrin polymer added to the background electrolyte at different pH in the range of 4.5-7. Furthermore, the effects of the concentration of the background electrolyte, column temperature, and applied voltage on chiral resolution were also examined. The best enantiomeric separations were obtained using a background electrolyte at pH 6 containing 100 mg/mL of beta-cyclodextrin polymer.
Istituto die Cromatografia del C.N.R., Area della Ricerca di Roma, Monterotondo Scalo, Italy.
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Aturki
D003505:Cyclodextrins / Q000494:pharmacology; D019075:Electrophoresis, Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D006880:Hydroxy Acids / Q000737:chemistry / Q000302:isolation & purification*; D007773:Lactates / Q000302:isolation & purification; D008333:Mandelic Acids / Q000302:isolation & purification; D011108:Polymers; D013237:Stereoisomerism; D047392:beta-Cyclodextrins
D003505:Cyclodextrins; D006880:Hydroxy Acids; D007773:Lactates; D008333:Mandelic Acids; D011108:Polymers; D047392:beta-Cyclodextrins; C014600:4-hydroxymandelic acid; C017648:3-phenyllactic acid; C024037:3-hydroxymandelic acid; C005434:atrolactic acid; C000989:3,4-dihydroxymandelic acid; C031215:betadex; C037938:mandelic acid