Detection of rDNA sites in sugarcane by FISH.
Hybridization sites of an rDNA probe coding for the 5.8S, 18S and 26S genes were detected on the chromosomes of sugarcane and a related genus, Erianthus, using fluorescence in situ hybridization. One unpaired and five paired hybridization sites were detected in a Saccharum spp. hybrid. A first introgression hybrid (I1) between Saccharum officinarum and Saccharum spontaneum had seven pairs of hybridization sites. A clone of Erianthus arundinaceus showed six hybridization sites in somatic tissue.
Bureau of Sugar Experiment Stations, Gordonvale, Australia.
M J
MJ
Jenkin
S M
SM
Reader
K A
KA
Purdie
T E
TE
Miller
D001315:Australia; D002875:Chromosomes / Q000235:genetics*; D015342:DNA Probes; D004275:DNA, Ribosomal / Q000235:genetics*; D005456:Fluorescent Dyes; D017404:In Situ Hybridization, Fluorescence; D007211:Indoles; D008540:Meiosis; D008677:Metaphase; D009697:Nucleolus Organizer Region / Q000235:genetics*; D010944:Plants / Q000235:genetics*
D015342:DNA Probes; D004275:DNA, Ribosomal; D005456:Fluorescent Dyes; D007211:Indoles; C007293:DAPI