In situ localization with digoxigenin-labelled probes of tau-related mRNAs in the rat pancreas.
Two cDNA probes complementary to fetal rat brain tau cDNA were produced by the polymerase chain reaction (PCR) and labelled by digoxigenin-11-dUTP incorporation during the PCR elongation step. These probes were tested for the in situ localization of tau mRNAs in sections of rat cerebellum. The hybridization signal was consistent with the known localization of brain tau mRNAs, showing the validity of cDNA probes labelled by digoxigenin during the PCR. Using these probes, an in situ hybridization protocol was established and optimized for the localization of tau-related mRNAs in sections of pancreas. The aim was to determine whether these mRNAs were expressed in the exocrine or the endocrine part of the pancreas. A positive signal was found only in the exocrine part of the pancreas, and was distributed exclusively in the cytoplasm of acinar cells. The results described here are the first evidence for a specific expression of tau-related proteins in the exocrine pancreas.
INSERM, Unité 381, Ontogénèse et pathologie du système digestif, Strasbourg, France.
P
P
Neuville
M T
MT
Vanier
L
L
Michalik
J F
JF
Launay
D000818:Animals; D002531:Cerebellum / Q000737:chemistry; D002846:Chromatography, Affinity; D015342:DNA Probes / Q000032:analysis; D003856:Deoxyuracil Nucleotides; D004076:Digoxigenin / Q000031:analogs & derivatives*; D017403:In Situ Hybridization; D007202:Indicators and Reagents; D008867:Microtomy; D010179:Pancreas / Q000737:chemistry*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis* / Q000235:genetics; D051381:Rats; D015203:Reproducibility of Results; D016875:tau Proteins / Q000032:analysis* / Q000235:genetics
D015342:DNA Probes; D003856:Deoxyuracil Nucleotides; D007202:Indicators and Reagents; D012333:RNA, Messenger; C070202:digoxigenin-11-deoxyuridine triphosphate; D016875:tau Proteins; D004076:Digoxigenin