B and T lymphocytes are enhanced in the gut of piglets fed heat-treated soyabean proteins.
Gut immune responses have been suspected in food hypersensitivity reactions such as those to soyabean proteins in early-weaned piglets. The present study examines the lymphoid cell subset distribution in piglets fed heat-treated (HTSP) or ethanol-treated soyabean proteins (ETSP). Duodenal cryosections of 4-week-old HTSP piglets (n = 10) and ETSP piglets (n = 8) were analysed for IgA, IgM, IgG1 and IgG2 positive cells, CD2, CD4, CD8, WC1 T cell positive antigens using immunohistochemical peroxidase reactions. Densities of IgM+ and IgA+ cells were three times and, IgG1+ and IgG2+ six times higher in the lamina propria of HTSP piglets compared with ETSP (P < 0.05). Increased CD2+ T cells were accounted for by a rise in both CD4+ and CD8+ T cell subsets in the lamina propria (P < 0.01) as well as in the epithelium of the duodenal mucosa of piglets fed HTSP. The density of the WC1+ T cell subset in the epithelium was significantly higher in HTSP than in ETSP piglets (P < 0.01). Immune reactions in the duodenal mucosa, involving both B and T lymphocytes may be related to atrophy of the duodenal villi in HTSP piglets.
Dréau
D
D
INRA, Laboratoire de Pathologie Infectieuse et Immunologie, Nouzilly, France.
Lallès
J P
JP
Toullec
R
R
Salmon
H
H
D000818:Animals; D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000032:analysis; D001402:B-Lymphocytes / Q000276:immunology*; D004044:Dietary Proteins / Q000008:administration & dosage / Q000009:adverse effects; D004386:Duodenum / Q000276:immunology; D005511:Food Handling; D005512:Food Hypersensitivity / Q000209:etiology / Q000276:immunology / Q000662:veterinary*; D006358:Hot Temperature; D007124:Immunoenzyme Techniques / Q000662:veterinary; D007132:Immunoglobulin Isotypes / Q000032:analysis; D016130:Immunophenotyping / Q000662:veterinary; D007413:Intestinal Mucosa / Q000276:immunology*; D018655:Lymphocyte Count / Q000662:veterinary; D014674:Plant Proteins, Dietary / Q000008:administration & dosage* / Q000009:adverse effects; D011897:Random Allocation; D030262:Soybean Proteins; D013025:Glycine max / Q000009:adverse effects; D013552:Swine; D013553:Swine Diseases / Q000209:etiology / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D004044:Dietary Proteins; D007132:Immunoglobulin Isotypes; D014674:Plant Proteins, Dietary; D030262:Soybean Proteins