Heterochromatin heterogeneity revealed by restriction endonuclease digestion and subsequent C-banding on bovine metaphase chromosomes.
Conventional alkaline C-banding was found to be an effective additional step in restriction endonuclease banding to improve staining contrast and to reveal hidden changes in the heterochromatic blocks. Examples of these cases are demonstrated on the bovine chromosome set using Hinf I and Taq I endonucleases in the digestion experiments.
Hidas
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Institute for Small Animal Research, Gödöllö, Hungary.
D000818:Animals; D002417:Cattle / Q000235:genetics*; D002871:Chromosome Banding / Q000662:veterinary*; D006570:Heterochromatin / Q000235:genetics*; D008677:Metaphase / Q000235:genetics*; D015183:Restriction Mapping
D006570:Heterochromatin