Effect of calcitonin, elevated calcium and extracellular matrices on superoxide anion production by rat osteoclasts.
The electrochemical determination of superoxide anion generation by isolated mammalian osteoclasts was carried out by employing cytochrome c, immobilized at a surface-modified gold electrode. Osteoclasts cultured on bone generated 15-fold more superoxide anions than cells plated on plastic. Superoxide anion production by osteoclasts cultured on bone was almost completely abolished by salmon calcitonin, and partially abolished following exposure to elevated extracellular calcium. We therefore conclude that osteoclast activity is influenced substantially by the extracellular matrix.
Department of Clinical Biochemistry, Medical School, Newcastle upon Tyne, UK.
H K
HK
Datta
P
P
Manning
H
H
Rathod
C J
CJ
McNeil
D000818:Animals; D000831:Animals, Newborn; D015374:Biosensing Techniques; D002116:Calcitonin / Q000494:pharmacology*; D002118:Calcium / Q000494:pharmacology*; D003574:Cytochrome c Group; D004566:Electrodes; D004800:Enzymes, Immobilized; D005109:Extracellular Matrix / Q000187:drug effects / Q000378:metabolism*; D010010:Osteoclasts / Q000378:metabolism*; D010101:Oxygen Consumption / Q000187:drug effects / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D013481:Superoxides / Q000378:metabolism*
D003574:Cytochrome c Group; D004800:Enzymes, Immobilized; D013481:Superoxides; C028815:salmon calcitonin; D002116:Calcitonin; D002118:Calcium