Surface localization, regulation, and biologic properties of the 96-kDa alcohol/aldehyde dehydrogenase (EhADH2) of pathogenic Entamoeba histolytica.
The 96-kDa surface antigen of Entamoeba histolytica was demonstrated through extensive immunologic evaluation with monoclonal and monospecific antibodies to be identical to or an isoform of the amebic alcohol/aldehyde dehydrogenase (EhADH2). EhADH2 was secreted, excreted, or shed into the culture medium in quantities commensurate with amebic growth when studied in a novel culture system. Of importance, using RNase protection assays, specific mRNA coding for the EhADH2 gene product(s) was up-regulated by treatment of viable trophozoites with the enzyme substrate ethanol. These data provide insight into the biology of this enzyme and its regulation by appropriate stressors.
College of Pharmacy, Department of Pharmaceutical Sciences, Idaho State University, Pocatello 83209-8334, USA.
B M
BM
Flores
S L
SL
Stanley
T S
TS
Yong
M
M
Ali
W
W
Yang
D L
DL
Diedrich
B E
BE
Torian
D000426:Alcohol Dehydrogenase / Q000276:immunology / Q000378:metabolism*; D000444:Aldehyde Dehydrogenase / Q000276:immunology / Q000378:metabolism*; D000818:Animals; D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan / Q000276:immunology / Q000378:metabolism*; D000954:Antigens, Surface / Q000276:immunology / Q000378:metabolism; D003429:Cross Reactions; D003470:Culture Media; D004591:Electrophoresis, Polyacrylamide Gel; D004748:Entamoeba histolytica / Q000187:drug effects / Q000201:enzymology* / Q000276:immunology / Q000472:pathogenicity; D000431:Ethanol / Q000494:pharmacology; D015151:Immunoblotting; D007527:Isoenzymes / Q000378:metabolism*; D008970:Molecular Weight; D012333:RNA, Messenger / Q000378:metabolism; D015531:Radioimmunoprecipitation Assay; D015854:Up-Regulation
D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan; D000954:Antigens, Surface; D003470:Culture Media; D007527:Isoenzymes; D012333:RNA, Messenger; D000431:Ethanol; D000426:Alcohol Dehydrogenase; D000444:Aldehyde Dehydrogenase