Modified crush-avulsion anastomosis model on the rat femoral vein.
A crush-avulsion anastomosis model was established on the rat femoral vein. Saline or heparin was used as a luminal topical agent and was allowed to contact the damaged endothelium for 10 min. Patency and coagulation parameters were investigated for 1 week. The heparin treated group had a patency rate of 93% at 1 hr vs. 13% for the saline treated group (P < 0.001). At 7 days, the heparin treated group had an 87% patency vs. 7% for the saline-treated group (P < 0.001). Scanning electron micrography (SEM) provided evidence of the deposition of the components of early thrombosis in the crushed venous wall. In contrast, the SEM of the heparin treated group shows a paucity of any evidence of thrombus. These results indicate that the rat vein crush-avulsion model is a reliable and reproducible thrombosis model with low patency. The methods used with the topical agent may improve the patency rate in crush avulsion injuries.
Division of Plastic Surgery, Loyola University Chicago, USA.
K
K
Fu
R
R
Izquierdo
T
T
Hubbard
J
J
Fareed
D000714:Anastomosis, Surgical / Q000379:methods; D000818:Animals; D005268:Femoral Vein / Q000293:injuries* / Q000601:surgery* / Q000648:ultrastructure; D005343:Fibrinolytic Agents / Q000627:therapeutic use*; D006493:Heparin / Q000627:therapeutic use*; D008855:Microscopy, Electron, Scanning; D051381:Rats; D017207:Rats, Sprague-Dawley; D012965:Sodium Chloride / Q000627:therapeutic use; D013536:Suture Techniques; D013927:Thrombosis / Q000517:prevention & control*; D014654:Vascular Patency / Q000187:drug effects
D005343:Fibrinolytic Agents; D012965:Sodium Chloride; D006493:Heparin