Evaluation of Dirofilaria immitis excretory/secretory products for seroepidemiological studies on human dirofilariosis.
The usefulness of excretory/secretory (E/S) products of adult Dirofilaria immitis for the diagnosis of human dirofilariosis was evaluated in 97 human sera using an ELISA and a Western Blot analysis with E/S antigens. Results of the ELISA analysis show that in healthy individuals from an endemic area seropositivity is high (22%). Immuno Blot analysis shows that the reaction pattern with E/S antigens differentiates healthy seropositives, which recognize a 43 kDa band, from patients with pulmonary dirofilariosis, which specifically recognize bands between 22-28 kDa.
Laboratorio de Parasitología, Facultad de Farmacia, Salamanca, Spain.
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Santamaría
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Cordero
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Muro
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F
Simón
D000818:Animals; D000909:Antibodies, Helminth / Q000032:analysis; D000947:Antigens, Helminth / Q000276:immunology*; D015153:Blotting, Western; D004183:Dirofilaria immitis / Q000276:immunology*; D004184:Dirofilariasis / Q000175:diagnosis* / Q000453:epidemiology; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D016036:Seroepidemiologic Studies
D000909:Antibodies, Helminth; D000947:Antigens, Helminth