Micronucleus frequencies in lymphoblastoid cell lines measured with the cytokinesis-block technique and flow cytometry.
In these experiments, the cytokinesis-block technique was used to measure the spontaneous and radiation-induced micronucleus frequencies in lymphoblastoid cell lines from normal individuals and individuals with radiation-sensitive syndromes. The radiation-induced micronucleus frequencies were measured at different harvest times to investigate the optimum time to measure radiation-induced cytogenetic damage with the cytokinesis-block technique. The frequency of radiation-induced micronuclei was found to be independent of the frequency of binucleate cells in these cell lines. Longer incubation times were complicated by increasing numbers of mononucleate and multinucleate cells, and scoring slides at an early incubation time after the maximum frequency of binucleate cells is reached was recommended. Two of the cell lines (one established from an individual with ataxia telangiectasia, and one from an infant with mitotic instability) had significantly higher radiation-induced micronucleus frequencies than the control cell lines. Finally, the radiation-induced micronucleus frequencies in three of the cell lines were measured by flow cytometry, and the results ranked the cell lines in the same order of radiosensitivity as the cytokinesis block technique.
Slavotinek
A
A
MRC Human Genetics Unit, Western General Hospital, Edinburgh, Germany.
Miller
E
E
Taylor
G M
GM
Nüsse
M
M
van Heyningen
V
V
D002455:Cell Division / Q000187:drug effects*; D002460:Cell Line; D002469:Cell Separation; D005434:Flow Cytometry; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects / Q000528:radiation effects* / Q000648:ultrastructure; D015162:Micronucleus Tests / Q000379:methods*; D011836:Radiation Tolerance