[Phosphatidylinositol specific phospholipase C (PI-PLC) in differentiation of Listeria monocytogenes and Listeria innocua].
Listeria strains were investigated as regard: the production of PI-PLC and lecytinase, haemolytic and CAMP activity survival in mouse tissues. Although all the examined L. monocytogenes strains produced PI-PLC, they showed a very different activity of this enzyme. It was surprising that one of the four L. innocua strains demonstrated a weak activity of PI-PLC. L. monocytogenes bacteria with strong PI-PLC activity survived in mouse spleens for a much longer period of time than L. monocytogenes microbes with only a weak production of this enzyme. However, L. innocua bacteria producing some PI-PLC were quickly eliminated by infected mice.
Zakład Biologii Infekcyjnej Uniwersytetu Lódzkiego.
M
M
Pachelska
B
B
Rózalska
J
J
Smola
W
W
Rudnicka
D000818:Animals; D005260:Female; D006801:Humans; D008087:Listeria / Q000145:classification / Q000201:enzymology* / Q000302:isolation & purification; D008089:Listeria monocytogenes / Q000201:enzymology; D008088:Listeriosis / Q000175:diagnosis / Q000382:microbiology; D051379:Mice; D008807:Mice, Inbred BALB C; D043265:Phosphatidylinositol Diacylglycerol-Lyase; D054801:Phosphoinositide Phospholipase C; D010727:Phosphoric Diester Hydrolases / Q000032:analysis*; D011817:Rabbits; D012756:Sheep; D013045:Species Specificity; D013154:Spleen / Q000382:microbiology
D010727:Phosphoric Diester Hydrolases; D054801:Phosphoinositide Phospholipase C; D043265:Phosphatidylinositol Diacylglycerol-Lyase
Fosfolipaza C fosfatydyloinozytolu (PI-PLC) w róznicowaniu Listeria monocytogenes i Listeria innocua.