Advances in human immunoglobulin allotypes.
Monoclonal anti-Rh's and monoclonal anti-Gm's are now available, permitting a more precise serological determination of the human allotypes. Several Gm allotypes can be determined at the gene level, without resort to serological reagents. PCR, subclass-specific amplification and allele-specific probes are used. Many RFLPs of the human IgH C- and V-region genes have now been defined. More than 10(6) variant haplotypes of the so-called constant part of the Ig chain are possible because more than 20 polymorphic sites are known. A schematic physical map of the human IgHC gene cluster is presented.
Grubb
R
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Department of Medical Microbiology, University of Lund, Sweden.
D000818:Animals; D002883:Chromosomes, Human, Pair 14; D002889:Chromosomes, Human, Pair 2; D003062:Codon / Q000235:genetics; D004198:Disease Susceptibility / Q000276:immunology; D005803:Genes, Immunoglobulin; D020022:Genetic Predisposition to Disease; D006239:Haplotypes / Q000235:genetics; D006801:Humans; D007126:Immunoglobulin Allotypes / Q000235:genetics*; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D007135:Immunoglobulin Variable Region / Q000235:genetics; D051379:Mice; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length
D003062:Codon; D007126:Immunoglobulin Allotypes; D007143:Immunoglobulin Heavy Chains; D007135:Immunoglobulin Variable Region