Site-directed mutagenesis revealed role of subsite residues of Mucor pusillus pepsin in catalytic function.
Park
Y N
YN
Department of Agricultural Chemistry, Faculty of Agriculture, University of Tokyo, Japan.
Nishiyama
M
M
Horinouchi
S
S
Beppu
T
T
D000595:Amino Acid Sequence; D002384:Catalysis; D003001:Cloning, Molecular; D007328:Insulin / Q000378:metabolism; D008969:Molecular Sequence Data; D009089:Mucor / Q000201:enzymology*; D016297:Mutagenesis, Site-Directed; D010434:Pepsin A / Q000096:biosynthesis / Q000737:chemistry* / Q000378:metabolism*; D017354:Point Mutation; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism; D013379:Substrate Specificity
D007328:Insulin; D011994:Recombinant Proteins; D010434:Pepsin A