Recognition of human recombinant myelin associated glycoprotein by anti-carbohydrate antibodies of the L2/HNK-1 family.
The L2 and HNK-1 monoclonal antibodies recognize carbohydrate determinants containing sulfate-3-glucuronate that are prominent on cells of neural crest lineages. In humans these epitopes are most abundant on the Myelin Associated Glycoprotein and it was assumed that they co-localize on the same molecules. Recently, in vitro synthesized carbohydrates have provided a basis for the different recognition requirements of these two antibodies. We now provide in vivo evidence that a human melanoma cell line can produce glycoproteins such as fibronectin, which is recognized by both the L2 and HNK-1 antibodies, and simultaneously a transfected Myelin Associated Glycoprotein carrying only L2-type carbohydrates. Conceivably, the differential expression of L2- and HNK-1 type glycans could have a role in development.
Bigliardi-Qi
M
M
Department of Clinical Neurology, University Hospitals, Basel, Switzerland.
Miescher
G C
GC
Steck
A J
AJ
D000911:Antibodies, Monoclonal; D002241:Carbohydrates / Q000737:chemistry / Q000276:immunology; D000939:Epitopes / Q000737:chemistry; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D008545:Melanoma / Q000276:immunology; D018992:Myelin-Associated Glycoprotein / Q000235:genetics / Q000276:immunology*; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D014162:Transfection; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D002241:Carbohydrates; D000939:Epitopes; D018992:Myelin-Associated Glycoprotein; D011994:Recombinant Proteins