Noradrenergic system in the chicken brain: immunocytochemical study with antibodies to noradrenaline and dopamine-beta-hydroxylase.
A light microscopic immunocytochemical study, using antisera against noradrenaline (NA) and dopamine-beta-hydroxylase (DBH), revealed the noradrenergic system in the brain of the chicken (Gallus domesticus). NA- and DBH-immunoreactive (ir) elements showed a similar distribution throughout the whole brain. The neurons immunoreactive for the monoamine were confined to the lower brainstem, the pons, and the medulla. In the pons, a rather dense group of cells was found in the dorsal, most posterior part of the locus coeruleus and in the caudal nucleus subcoeruleus ventralis. A few labeled cells appeared in and around the nucleus olivaris superior in the most caudal part of the metencephalic tegmentum. In the medulla oblongata, noradrenergic cells could be visualized at the level of the nucleus of the solitary tract and in a ventrolateral complex. Virtually all regions of the brain contained a rather dense innervation by NA- and DBH-immunopositive varicose fibers. Noradrenergic fibers and terminals were especially abundant in the ventral forebrain and in the periventricular hypothalamic regions. DBH-ir and NA-ir fibers, varicosities, and punctate structures could be observed in close association with immunonegative perikarya in several brain regions, more specifically in the ventral telencephalon, in the mid- and tuberal hypothalamic region, and in the dorsal rostral pons. Some perikarya in these brain areas were completely surrounded by noradrenergic structures that formed pericellular arrangements around the cells. The present study on the distribution of the noradrenergic system in the brain of the chicken combined with the results of a previous report on the distribution of L-Dopa and dopamine in the same species (L. Moons, J. van Gils, E. Ghijsels, and F. Vandesande, 1994, J. Comp. Neurol. 346:97-118) offers the opportunity to differentiate between the various catecholamines in the brain of this vertebrate. The results are discussed in relation to catecholaminergic systems previously reported in avian species and in the mammalian brain.
Laboratory of Neuroendocrinology, Zoological Institute, Leuven, Belgium.
L
L
Moons
E
E
D'Hondt
K
K
Pijcke
F
F
Vandesande
D000320:Adrenergic Fibers / Q000276:immunology* / Q000648:ultrastructure*; D000818:Animals; D000906:Antibodies / Q000276:immunology*; D002645:Chickens; D004298:Dopamine / Q000378:metabolism*; D007150:Immunohistochemistry; D008526:Medulla Oblongata / Q000648:ultrastructure; D006899:Mixed Function Oxygenases / Q000276:immunology*; D009434:Neural Pathways / Q000502:physiology*; D013687:Telencephalon / Q000648:ultrastructure
D000906:Antibodies; D006899:Mixed Function Oxygenases; D004298:Dopamine