A method for immunoisolation of sporocysts of Schistosoma mansoni in nonsusceptible snails.
A technique is described for immunoisolation of sporocysts of Schistosoma mansoni in nonsusceptible Biomphalaria glabrata by microencapsulation in agarose. Based on histological evidence, all 11 microencapsulated sporocysts implanted into 3 schistosome-resistant 13-16-R1 snails were alive at 72 hr postimplantation, although in a developmentally retarded condition. However, among 146 sporocysts derived from miracidia that had penetrated 5 snails, 96% were dead by 72 hr. These results suggest that hemocyte contact is necessary for rapid sporocyst death in vivo.
Department of Biology, Lamar University, Beaumont, Texas 77710, USA.
J T
JT
Sullivan
P C
PC
Hu
D000818:Animals; D001700:Biomphalaria / Q000276:immunology* / Q000469:parasitology; D004339:Drug Compounding; D012550:Schistosoma mansoni / Q000276:immunology* / Q000472:pathogenicity; D013514:Surgical Procedures, Operative / Q000379:methods / Q000662:veterinary