A novel factor-dependent human myelodysplastic cell line, MDS92, contains haemopoietic cells of several lineages.
A novel long-term cultured interleukin (IL)-3-dependent human myelodysplastic cell line, MDS92, was shown to contain several myeloid-lineage cells such as neutrophils, macrophages, eosinophils, and a small number of megakaryocyte-lineage cells. Therefore this cell line possesses at least bipotential characteristics of myeloid- and megakaryocyte-lineages. Granulocyte colony-stimulating factor clearly promoted the neutrophil alkaline phosphatase activity of MDS92 cells. To the contrary, the incidence and growth of CD41-positive cells were hardly affected by the addition of IL-6, IL-11, c-mpl ligand (thrombopoietin, TPO) or erythropoietin. TPO slightly supported the growth of CD34-positive cell fraction, but not CD41-positive cell fraction of MDS92 cells in combination with IL-3 or Steel factor. This cell line will be a useful tool for the study of MDS stem cells, but the mechanism of commitment of differentiation in MDS stem cells remains unknown.
Tohyama
K
K
First Department of Internal Medicine, Fukui Medical School, Japan.
Tohyama
Y
Y
Nakayama
T
T
Ueda
T
T
Nakamura
T
T
Yoshida
Y
Y
D000469:Alkaline Phosphatase / Q000378:metabolism; D015703:Antigens, CD / Q000032:analysis; D015815:Cell Adhesion Molecules / Q000494:pharmacology; D002455:Cell Division; D002460:Cell Line; D019070:Cell Lineage; D004804:Eosinophils / Q000473:pathology; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology; D006412:Hematopoietic Stem Cells / Q000276:immunology / Q000473:pathology* / Q000502:physiology; D006801:Humans; D007377:Interleukin-3 / Q000494:pharmacology* / Q000502:physiology; D008264:Macrophages / Q000473:pathology; D008533:Megakaryocytes / Q000276:immunology / Q000473:pathology; D009190:Myelodysplastic Syndromes / Q000473:pathology*; D009504:Neutrophils / Q000187:drug effects / Q000201:enzymology / Q000473:pathology*; D019089:Stem Cell Factor / Q000494:pharmacology; D013926:Thrombopoietin / Q000494:pharmacology
D015703:Antigens, CD; D015815:Cell Adhesion Molecules; D007377:Interleukin-3; D019089:Stem Cell Factor; D016179:Granulocyte Colony-Stimulating Factor; D013926:Thrombopoietin; D000469:Alkaline Phosphatase