Competition for peptides and amino acids among periodontal bacteria.
We recently studied the utilization of glutathione (L-gamma-glutamyl-L-cysteinylglycine), L-cysteinylglycine and L-cysteine by anaerobic bacteria. The rate of hydrogen sulfide formation from these compounds was determined and it was concluded that Peptostreptococcus micros and Fusobacterium nucleatum subsp. nucleatum had an active transport of small peptides. In the present study it is shown that methyl mercaptan formation from L-methionine and L-methionyl-containing peptides can also be used to study peptide utilization. There were differences among the periodontal bacteria P. micros, F. nucleatum subsp. nucleatum, and Porphyromonas gingivalis in their capacity to use L-cysteine and L-methionine and peptides containing these amino acids. The peptides were used more efficiently by P. micros and F. nucleatum subsp. nucleatum than by P. gingivalis. All three species used the peptides more efficiently than the free amino acids. The efficiency in utilizing various amino acids and peptides may be among the key determinants of the periodontal microbial ecology.
Department of Oral Microbiology, University of Umeå, Sweden.
J
J
Tang-Larsen
R
R
Claesson
M B
MB
Edlund
J
J
Carlsson
D000603:Amino Acids, Sulfur / Q000378:metabolism*; D001421:Bacteria, Anaerobic / Q000378:metabolism*; D001692:Biological Transport; D013437:Carbon-Sulfur Lyases / Q000378:metabolism; D003542:Cystathionine gamma-Lyase / Q000378:metabolism; D003545:Cysteine / Q000378:metabolism; D004151:Dipeptides / Q000378:metabolism; D017753:Ecosystem; D016967:Fusobacterium nucleatum / Q000378:metabolism; D006862:Hydrogen Sulfide / Q000378:metabolism; D008715:Methionine / Q000378:metabolism; D009842:Oligopeptides / Q000378:metabolism*; D010462:Peptostreptococcus / Q000378:metabolism; D010514:Periodontal Pocket / Q000382:microbiology*; D016966:Porphyromonas gingivalis / Q000378:metabolism; D013438:Sulfhydryl Compounds / Q000378:metabolism
D000603:Amino Acids, Sulfur; D004151:Dipeptides; D009842:Oligopeptides; D013438:Sulfhydryl Compounds; C005231:methylmercaptan; C028505:cysteinylglycine; C034758:methionylmethionine; D008715:Methionine; D013437:Carbon-Sulfur Lyases; D003542:Cystathionine gamma-Lyase; C021558:L-methionine gamma-lyase; D003545:Cysteine; D006862:Hydrogen Sulfide