Sensitivity and specificity of an isoelectric focusing fraction of Blastomyces dermatitidis yeast lysate antigen for the detection of canine blastomycosis.
Blastomyces dermatitidis yeast lysate antigen (T-58, dog isolate) fractions prepared using the Rotofor preparative isoelectric focusing (IEF) cell (Bio-Rad) were compared with B. dermatitidis yeast lysate and filtrate reagents with respect to the detection of antibodies in sera from dogs with blastomycosis, histoplasmosis, coccidioidomycosis, cryptococcosis and aspergillosis. A horseradish peroxidase enzyme immunoassay with Turbo TMB substrate was used in the study. One particular IEF fraction (pH 4.3) was optimal in the assay, and it exhibited greater sensitivity (100%) and specificity (93%) than the lysate or filtrate preparations. The highest degree of cross-reactivity was encountered with the histoplasmosis and coccidioidomycosis specimens and considerably less with the cryptococcosis and aspergillosis sera. Studies are in progress to purify further the optimal IEF fraction.
Fisher
M A
MA
Department of Biological Sciences, Idaho State University, Pocatello, USA.
Bono
J L
JL
Abuodeh
R O
RO
Legendre
A M
AM
Scalarone
G M
GM
D000818:Animals; D000908:Antibodies, Fungal / Q000097:blood* / Q000276:immunology; D000946:Antigens, Fungal / Q000276:immunology / Q000302:isolation & purification; D001228:Aspergillosis / Q000662:veterinary; D001759:Blastomycosis / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary*; D003047:Coccidioidomycosis / Q000662:veterinary; D003429:Cross Reactions; D003453:Cryptococcosis / Q000662:veterinary; D004283:Dog Diseases / Q000175:diagnosis*; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006660:Histoplasmosis / Q000662:veterinary; D007525:Isoelectric Focusing; D009024:Morphogenesis; D012680:Sensitivity and Specificity; D015003:Yeasts / Q000276:immunology
D000908:Antibodies, Fungal; D000946:Antigens, Fungal