The genes for a spliceosome protein (SAP62) and the anti-Müllerian hormone (AMH) are contiguous.
During an investigation of the regulatory potential of a region 5' of the mouse anti-müllerian hormone (Amh) gene, we identified a region of homology with the known cDNA sequence of a human spliceosome gene (SAP62). In mouse, the Sap62 termination codon (TGA) is just 434 bp 5' of the Amh start of translation (ATG); in the human the equivalent distance is 789 bp. RNase protection analysis shows the majority of Sap62 transcripts use an uncommon polyadenylation signal (ATTAAA) lying in the intragenic region, 87 bp 3' of the TGA. This analysis also shows that Sap62 is transcribed in all tissues examined, whereas specific Amh transcription initiating 10 bp 5' of the ATG is limited to the developing testis of the fetus from 11.5 days post coitum and in the ovary from 3 days post partum. However, in all tissues a significant number of Sap62 transcripts fail to polyadenylate in the intragenic region and continue through the Amh locus. This implies that the Amh locus is in an open chromatin state in all tissues despite a requirement for precise regulation. Human SAP62 can now be mapped to HSA19p and mouse Sap62 to MMU10.
Dresser
D W
DW
Laboratory of Cellular Immunology, National Institute for Medical Research, Mill Hill, London, UK.
Hacker
A
A
Lovell-Badge
R
R
Guerrier
D
D
D000595:Amino Acid Sequence; D000818:Animals; D054304:Anti-Mullerian Hormone; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D004247:DNA; D006023:Glycoproteins; D006131:Growth Inhibitors / Q000235:genetics*; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009095:Mullerian Ducts / Q000378:metabolism*; D009698:Nucleoproteins / Q000235:genetics*; D000072260:RNA Splicing Factors; D016601:RNA-Binding Proteins; D017386:Sequence Homology, Amino Acid; D013735:Testicular Hormones / Q000235:genetics*; D016335:Zinc Fingers
D002352:Carrier Proteins; D006023:Glycoproteins; D006131:Growth Inhibitors; D009698:Nucleoproteins; D000072260:RNA Splicing Factors; D016601:RNA-Binding Proteins; C083433:SF3A2 protein, human; D013735:Testicular Hormones; D054304:Anti-Mullerian Hormone; D004247:DNA