Short-chain phospholipids enhance amphipathic peptide-mediated gene transfer.
Addition of short-chain phospholipids to the gramicidin S-DNA-dioleoyl phosphatidylethanolamine complex enhanced up to 6-fold beta-galactosidase expression in several cell-lines in vitro. Among the compounds tested, the most potent in enhancing transfection were the dicapryl- and the dicapryloyl phosphatidylcholine. In contrast, no significant enhancement of transfection was seen when short-chain phospholipids were mixed with cationic lipids. Short-chain phospholipid and gramicidin S may act simultaneously on the cell membrane to enhance gene transfer, yet resulting in no toxicity.
Drug Delivery Systems Section, F. Hoffmann-La Roche, Basel, Switzerland.
J Y
JY
Legendre
A
A
Supersaxo
D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000187:drug effects; D004247:DNA / Q000235:genetics; D018014:Gene Transfer Techniques; D006096:Gramicidin / Q000494:pharmacology; D006801:Humans; D051379:Mice; D010713:Phosphatidylcholines / Q000494:pharmacology; D010714:Phosphatidylethanolamines / Q000494:pharmacology; D010743:Phospholipids / Q000737:chemistry / Q000494:pharmacology*; D013329:Structure-Activity Relationship; D001616:beta-Galactosidase / Q000235:genetics
D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D010743:Phospholipids; C015132:1,2-didecanoylphosphatidylcholine; D006096:Gramicidin; C020888:1,2-dielaidoylphosphatidylethanolamine; D004247:DNA; D001616:beta-Galactosidase