Differential involvement of phospholipase C isozymes in Alzheimer's disease.
Phosphoinositide-specific phospholipase C (PLC) is a key enzyme in signal transduction. We demonstrated that an antibody to an isozyme of PLC, PLC-delta 1, produced intense staining of neurofibrillary tangles (NFT) in the brains of patients with Alzheimer's disease (AD). Western blot analysis using a specific antibody for PLC-delta 1 showed that the concentration of PLC-delta 1 protein was significantly higher in the cytosolic fraction of AD cortical tissues than in controls. The activity of PLC-delta 1, which hydrolyzes phosphatidylinositol, was not significantly different in the Alzheimer and control cytosolic fraction, indicating that the specific activity of PLC-delta 1 is decreased in Alzheimer brains. Western blot analysis also showed that the concentration of PLC-delta 1 and PLC-gamma 1 was significantly lower in the membraneous fraction of AD cortical tissues than in controls. These results suggest a differential involvement of PLC isozymes in AD.
Department of Neurology, Faculty of Medicine, Kyoto University, Japan.
S
S
Shimohama
H
H
Matsushima
S
S
Fujimoto
T
T
Takenawa
T
T
Taniguchi
M
M
Kameyama
J
J
Kimura
D000368:Aged; D000544:Alzheimer Disease / Q000503:physiopathology*; D001921:Brain / Q000201:enzymology; D002102:Cadaver; D006801:Humans; D007120:Immunochemistry; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000502:physiology*; D010738:Type C Phospholipases / Q000502:physiology*
D007527:Isoenzymes; D010738:Type C Phospholipases