Culture microtitration: a sensitive method for quantifying Leishmania infantum in tissues of infected mice.
We developed a microtitration method to determine the parasite burdens in homogenized organs of mice infected with Leishmania infantum. This method proved more sensitive than direct enumeration of amastigotes in stained organs, was appropriate for describing the kinetics of infection, and can be considered for physiopathological or pharmaceutical experimental studies.
Laboratoire de Parasitologie-Mycologie, Faculté de Médecine Lariboisière Saint-Louis, Paris, France.
P A
PA
Buffet
A
A
Sulahian
Y J
YJ
Garin
N
N
Nassar
F
F
Derouin
D000818:Animals; D005260:Female; D007700:Kinetics; D018314:Leishmania infantum; D007898:Leishmaniasis, Visceral / Q000469:parasitology*; D008099:Liver / Q000469:parasitology; D051379:Mice; D008807:Mice, Inbred BALB C; D013154:Spleen / Q000469:parasitology