Molecular typing of Salmonella enterica subsp. enterica serovar Enteritidis isolates.
A collection of 31 epidemiologically unrelated Salmonella enterica subsp. enterica serovar Enteritidis (S. Enteritidis) isolates obtained during a 12-year period was characterised by different molecular typing methods. Plasmid profile analysis, the detection of plasmid-encoded virulence genes and ribotyping allowed little or no further differentiation amongst these isolates. Two different hybridisation patterns were observed by IS200-typing of the S. Enteritidis isolates. However, pulsed-field gel electrophoretic separation of restriction endonuclease-digested whole-cell DNA provided a high level of discrimination amongst the 31 S. Enteritidis isolates. This could be increased by the comparative use of the three suitable restriction endonucleases XbaI, SpeI and NotI. Thus, pulsed-field gel electrophoresis proved to be superior in its discriminatory value to other molecular methods such as plasmid analysis, ribotyping or IS200-typing and represents a most helpful tool for the epidemiological typing of S. Enteritidis isolates.
Institut für Kleintierforschung, Bundesforschungsanstalt für Landwirtschaft (FAL), Celle, Germany.
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Liebisch
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Schwarz
D000818:Animals; D001434:Bacteriophage Typing; D004269:DNA, Bacterial / Q000032:analysis*; D004352:Drug Resistance, Microbial; D016521:Electrophoresis, Gel, Pulsed-Field; D006801:Humans; D009693:Nucleic Acid Hybridization; D010957:Plasmids / Q000737:chemistry / Q000235:genetics; D012150:Polymorphism, Restriction Fragment Length; D011200:Poultry; D015347:RNA Probes; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012477:Salmonella enteritidis / Q000145:classification* / Q000187:drug effects / Q000235:genetics; D014774:Virulence / Q000235:genetics
D004269:DNA, Bacterial; D015347:RNA Probes; D012336:RNA, Ribosomal, 16S