Assay of cholinesterase using a pro-enhancer of the luminol-H2O2-horseradish peroxidase reaction.
2-Naphthyl acetate acts as a pro-enhancer of the luminol-H2O2-horseradish peroxidase reaction. Cholinesterase hydrolyses the bound acetyl group and produces 2-naphthol, and this compound is an enhancer of the chemiluminescent reaction. We studied the kinetics of chemiluminescent emission and the influence of 2-naphthyl acetate and cholinesterase enzyme concentrations. The cholinesterase concentration versus chemiluminescence intensity maximum was linear for cholinesterase between 0 and 181 microU/mL, with a detection limit of 8 microU/mL and a relative standard deviation of 9.5% (n = 3), for a sample containing 90.67 microU/mL of cholinesterase.
Department of Analytical Chemistry, Faculty of Sciences, University of Málaga, Spain.
A
A
Navas Díaz
F
F
García Sanchez
J A
JA
González García
V
V
Bracho Del Rio
D002802:Cholinesterases / Q000032:analysis* / Q000097:blood / Q000378:metabolism; D006735:Horseradish Peroxidase; D006801:Humans; D006861:Hydrogen Peroxide; D007202:Indicators and Reagents; D007700:Kinetics; D008163:Luminescent Measurements; D008165:Luminol; D009284:Naphthols / Q000494:pharmacology*; D012680:Sensitivity and Specificity
D007202:Indicators and Reagents; D009284:Naphthols; C005366:naphthyl acetate; D008165:Luminol; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase; D002802:Cholinesterases