Evaluation in vitro and in vivo of cationic liposome-expression construct complexes for cystic fibrosis gene therapy.
We have tested the cationic liposome N-(1-(2,3-dioleoyloxy)propyl)-N,N,N-trimethyl-ammoniummethylsul phate, (DOTAP), for gene delivery in vitro and in vivo with a view to clinical use in gene therapy for cystic fibrosis. Delivery of lacZ cDNA-DOTAP complexes via aerosol showed promoter-dependent differences in the pattern and longevity of expression. Repeated administration was well tolerated. The potential for the transfer of foreign genes into reproductive tissue was investigated by intravenous injection of DNA-DOTAP into female mice. Foreign DNA was undetectable in the ovaries by Southern blot analysis at 1 and 7 days after injection. Our results suggest that DOTAP merits testing in cystic fibrosis patients for delivery of the cystic fibrosis transmembrane conductance regulator (CFTR) gene to the respiratory tract and that substitution of the cytomegalovirus (CMV) promoter for the simian virus (SV) promoter may improve on the transitory response reported previously.
MRC Human Genetics Unit, Western General Hospital, Edinburgh, Scotland.
G
G
McLachlan
D J
DJ
Davidson
B J
BJ
Stevenson
P
P
Dickinson
H
H
Davidson-Smith
J R
JR
Dorin
D J
DJ
Porteous
D000336:Aerosols; D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D003550:Cystic Fibrosis / Q000628:therapy*; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000096:biosynthesis / Q000235:genetics* / Q000633:toxicity; D004274:DNA, Recombinant / Q000008:administration & dosage / Q000032:analysis / Q000235:genetics; D005229:Fatty Acids, Monounsaturated / Q000633:toxicity; D005260:Female; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods*; D006801:Humans; D008081:Liposomes; D008168:Lung / Q000737:chemistry / Q000502:physiology; D008297:Male; D051379:Mice; D010053:Ovary / Q000737:chemistry; D011401:Promoter Regions, Genetic; D000644:Quaternary Ammonium Compounds / Q000633:toxicity; D013737:Testis / Q000737:chemistry; D001616:beta-Galactosidase / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics
D000336:Aerosols; C505032:CFTR protein, human; D004274:DNA, Recombinant; D005229:Fatty Acids, Monounsaturated; D008081:Liposomes; D000644:Quaternary Ammonium Compounds; D019005:Cystic Fibrosis Transmembrane Conductance Regulator; D001616:beta-Galactosidase; C070046:1,2-dioleoyloxy-3-(trimethylammonium)propane