Sequence of the flounder (Paralichthys olivaceus) growth hormone-encoding gene and its promoter region.
The flounder (Paralichthys olivaceus) growth hormone (GH)-encoding gene (fGH) and its promoter region were cloned and sequenced following amplification of genomic DNA by the polymerase chain reaction. The fGH gene is 2.1-kb long and consists of six exons and five introns. In the 5'-flanking region of the determined transcription start point, a potential TATA box is located at -24, and Pit-1/GHF-1-binding site candidates are located in the -70 to -53 and -133 to -141 regions.
Tanaka
M
M
Department of Biochemistry, Faculty of Medicine, Mie University, Japan.
Toma
Y
Y
Ohkubo
T
T
Sudo
S
S
Nakashima
K
K
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000235:genetics; D005091:Exons / Q000235:genetics; D005432:Flounder / Q000235:genetics*; D005796:Genes / Q000235:genetics*; D013006:Growth Hormone / Q000235:genetics*; D007438:Introns / Q000235:genetics; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics*; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D016385:TATA Box; D050817:Transcription Factor Pit-1; D014157:Transcription Factors / Q000235:genetics; D014158:Transcription, Genetic / Q000235:genetics
D004268:DNA-Binding Proteins; D050817:Transcription Factor Pit-1; D014157:Transcription Factors; D013006:Growth Hormone