Routine high-performance liquid chromatographic determination of myocardial interstitial norepinephrine.
The present study describes a high-performance liquid chromatographic-electrochemical detection (HPLC-ED) system for routine measurement of the low levels of norepinephrine (NE) found in the myocardial interstitial space. In this system, an in vivo detection limit of 100 fg in a 50-microliters injection was achieved for NE. Using cardiac dialysis technique, 20-microliters dialysates were sampled from the myocardial interstitial space at 2-min intervals. The basal dialysate NE concentration was 16.6 +/- 4.0 pg/ml. This low detection limit allowed the dialysate NE concentration to be monitored for dysfunction of the cardiac sympathetic nerve terminal. This system offers a new possibility for routine analysis of myocardial interstitial NE levels.
Department of Cardiac Physiology, National Cardiovascular Center Research Institute, Osaka, Japan.
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Yamazaki
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Akiyama
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Shindo
D000818:Animals; D002415:Cats; D002851:Chromatography, High Pressure Liquid; D003956:Dialysis; D004563:Electrochemistry; D005260:Female; D008297:Male; D009206:Myocardium / Q000737:chemistry*; D009638:Norepinephrine / Q000032:analysis*; D013233:Stellate Ganglion / Q000378:metabolism; D013564:Sympathetic Nervous System / Q000378:metabolism
D009638:Norepinephrine