Role of the pyrimidine-rich tract on the translation of 'chimeric' polio-hepatitis A mRNAs with engineered 5'-terminal untranslated regions.
The 5'-terminal untranslated region (5'-UTR) of picornavirus RNA contains a series of cis-acting elements required for the internal initiation of translation, including a pyrimidine-rich tract (PRT), which in entero- and rhinoviruses is located about 20 nts upstream from a silent AUG triplet in the vicinity of the translation initiation site. In hepatitis A virus (HAV) RNA, the PRT is only 12 nts upstream from the legitimate AUG initiation codon, and a second, longer PRT in a region far removed from the translation initiation site. This 5'-distal PRT includes a 'core' sequence 80% homologous to the PRT of poliovirus RNA. A 'chimeric' polio-hepatitis A mRNA was constructed in which the sequences extending between nucleotides 45 and 156 of HAV RNA replaced the corresponding ones in poliovirus 5'-UTR. The construction was extended with poliovirus sequences up to position 1809. The recombinant mRNA so generated carried two copies of the PRT. In vitro translation in lysates of truncated poliovirus mRNAs generated a single peptide of Mr = 39 kDa, while the chimeric mRNA generated a series of short peptides as a result of fortuitous (or aberrant) initiation events. A more extensive substitution in the chimeric 5'-UTR which removed the 3'-most PRT brought by the poliovirus sequences, restored the translation at the authentic initiation site. Point mutations were engineered in the 5'-most PRT of the chimeras, and bi-cistronic plasmids were constructed in which either the parental poliovirus 5'-UTR or the chimeric ones were introduced in the intergenic region. Upon transfection of COS-1 cells, the chimeric polio-hepatitis A 5'-UTR containing two PRTs did not express the reporter gene. Removal of the 3'-distal PRT or point mutations engineered into the 5'-most PRT partially restored the transient expression of the reporter gene, consistent with the notion that a single (and only a single) functionally active PRT in a proper context is required to secure the internal initiation of translation of bi-cistronic mRNAs in vivo.
Department of Cellular and Developmental Biology, University of Rome, Italy.
A M
AM
Degener
J
J
Silveira Carneiro
C
C
Cassetti
A
A
Pierangeli
P
P
Pagnotti
M
M
Bucci
R
R
Perez Bercoff
D001483:Base Sequence; D002460:Cell Line; D015967:Gene Expression Regulation, Viral; D006507:Hepatovirus / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D017955:Poliovirus / Q000235:genetics*; D014176:Protein Biosynthesis; D011743:Pyrimidines; D012333:RNA, Messenger / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics*; D011995:Recombination, Genetic
D011743:Pyrimidines; D012333:RNA, Messenger; D012367:RNA, Viral