Removal of human immunodeficiency virus by an 0.04-micron membrane filter.
We tested the ability of a 0.04-micron nylon membrane filter to remove human immunodeficiency virus (HIV) from tissue culture media containing 10% fetal calf serum. Endpoint titrations of infectious virus (ID50) were performed on lymphocyte cultures. The presence of virus in the cultures was determined using an enzyme-linked immunoabsorbant assay (ELISA) of the HIV-1 P24 core antigen. In repeated experiments, filtration of the virus suspension resulted in the removal of HIV below detectable limits. The titer reduction was estimated to be greater than 8.5 x 10(2). These results suggest that this filter is effective in removing HIV from fluids containing serum or serum products.
Oshima
K H
KH
Biological Products Branch, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA.
Comans
T W
TW
Highsmith
A K
AK
Ades
E W
EW
D003470:Culture Media; D004797:Enzyme-Linked Immunosorbent Assay; D005374:Filtration / Q000379:methods; D016655:HIV Core Protein p24 / Q000032:analysis; D015497:HIV-1 / Q000302:isolation & purification*; D006801:Humans; D008214:Lymphocytes / Q000821:virology; D008567:Membranes, Artificial; D008851:Micropore Filters; D009757:Nylons; D013601:T-Lymphocytes / Q000821:virology
D003470:Culture Media; D016655:HIV Core Protein p24; D008567:Membranes, Artificial; D009757:Nylons