Collision-induced dissociation of multiply charged peptides in an ion-trap storage/reflectron time-of-flight mass spectrometer.
Single-frequency collisional activation of multiply charged peptides has been studied via electrospray ionization in an ion trap storage/reflectron time-of-flight device (IT/reTOF). Several peptides with molecular weights ranging from 600 to 1700 were used to demonstrate that sequence information can be obtained with this hybrid instrument with a sensitivity in the low picomole level. Further, a resolution of nearly 1000 can be obtained for the fragment products, due to the cooling effects of the buffer gas in the trap before analysis by the reTOF. The influence of the primary structure of the peptides on the observed collision-induced fragmentation patterns is discussed. Although the current study is limited by the electronics, in terms of its ion isolation capabilities, it is demonstrated that the device has the potential for obtaining sequence information for peptides with excellent sensitivity and relatively high resolution.
Department of Chemistry, University of Michigan, Ann Arbor 48109, USA.
M G
MG
Qian
Y
Y
Zhang
D M
DM
Lubman
D000595:Amino Acid Sequence; D000809:Angiotensins / Q000032:analysis; D001920:Bradykinin / Q000032:analysis; D004399:Dynorphins / Q000032:analysis; D004743:Enkephalin, Leucine / Q000032:analysis; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009496:Neurotensin / Q000032:analysis; D010446:Peptide Fragments / Q000032:analysis; D010455:Peptides / Q000737:chemistry*
D000809:Angiotensins; D010446:Peptide Fragments; D010455:Peptides; D009496:Neurotensin; D004743:Enkephalin, Leucine; D004399:Dynorphins; D001920:Bradykinin