Evaluation of the biodistribution and in vivo biochemistry of 99mTc-cysteine and 99/99mTc-cysteine complexes--a potential renal imaging agent.
Cysteine was chelated with 99mTc and/or 99Tc in a freeze-dried kit containing Tin(II) ions, and the yellow 99m/99Tc-cysteine complex (complex I) was separated to study the biodistribution. Comparison of the distribution of 99m/99Tc-cysteine and 99mTc-cysteine complexes was made in rats and mice. The renal excretion patterns were studied in rats in the presence and absence of the renal tubular transport inhibitor 2,4-dinitrophenol. The carrier of Tc-cysteine complex in the blood and also the radioactive compounds in the urine were studied by HPGFC and SDS-electrophoresis. The kidney was confirmed as the target organ; serum albumin serves as a carrier for transport of Tc-cysteine complex to the kidney. The Tc-cysteine complex was the primary form in excreta, and glomerular filtration was the dominant excretory pathway.
Department of Radiological Technology, Yuanpei Junior College of Medical Technology, Hsinchu City, Taiwan, R.O.C.
A Y
AY
Wang
R S
RS
Liu
D000818:Animals; D001345:Autoradiography; D001798:Blood Proteins / Q000378:metabolism; D003545:Cysteine / Q000031:analogs & derivatives* / Q000097:blood / Q000493:pharmacokinetics; D004337:Drug Carriers; D007668:Kidney / Q000000981:diagnostic imaging* / Q000378:metabolism; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D015609:Organotechnetium Compounds / Q000097:blood / Q000493:pharmacokinetics*; D011485:Protein Binding; D011877:Radionuclide Imaging; D051381:Rats; D017207:Rats, Sprague-Dawley; D014018:Tissue Distribution
D001798:Blood Proteins; D004337:Drug Carriers; D015609:Organotechnetium Compounds; D003545:Cysteine