The varicella-zoster virus immediate-early 62 promoter contains a negative regulatory element that binds transcriptional factor NF-Y.
The varicella-zoster virus (VZV) immediate-early (IE) open reading frame 62 (ORF62) promoter sequence contains a CCAAT box, located between positions -117 and -113. Mutation of this motif resulted in a five-fold increase of promoter activity. In mobility shift assays, we demonstrated that NF-Y, a CCAAT box-binding protein critical for the expression of diverse eukaryotic genes, binds to this motif. Thus, the VZV IE ORF62 promoter contains a negative regulatory element that binds the ubiquitous transcriptional factor NF-Y.
Medical Virology Section, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892, USA.
H
H
Moriuchi
M
M
Moriuchi
J I
JI
Cohen
D001483:Base Sequence; D022762:CCAAT-Enhancer-Binding Proteins; D004279:DNA, Viral / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D014645:Herpesvirus 3, Human / Q000235:genetics*; D006801:Humans; D017874:Immediate-Early Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D011401:Promoter Regions, Genetic; D015534:Trans-Activators / Q000235:genetics*; D014157:Transcription Factors / Q000378:metabolism*; D014407:Tumor Cells, Cultured; D014759:Viral Envelope Proteins / Q000235:genetics*
D022762:CCAAT-Enhancer-Binding Proteins; D004279:DNA, Viral; D004268:DNA-Binding Proteins; C074221:IE62 protein, Human herpesvirus 3; D017874:Immediate-Early Proteins; D015534:Trans-Activators; D014157:Transcription Factors; D014759:Viral Envelope Proteins