Viable porcine hepatocytes from slaughterhouse organs.
A modified enzymatic isolation technique for the successful harvesting of porcine liver cells from slaughterhouse organs is introduced. Digestion of the left medial liver lobe (n = 74) resulted in 1.0 +/- 0.3 x 10E7 viable hepatocytes per gram tissue and an overall yield of 1.92 +/- 0.5 x 10E9 cells per isolation (viability: 93 +/- 2%).
Department of Surgery, Klinikum Grosshadern, L.M. University of Munich, F.R. Germany.
H G
HG
Koebe
J
J
Mueller-Hoecker
S
S
Koebe
F W
FW
Schildberg
D000003:Abattoirs; D000410:Alanine Transaminase / Q000378:metabolism; D000418:Albumins / Q000378:metabolism; D000818:Animals; D001646:Bile / Q000378:metabolism; D002469:Cell Separation / Q000379:methods; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured / Q000166:cytology / Q000201:enzymology; D003374:Coumarins / Q000378:metabolism; D004247:DNA / Q000096:biosynthesis; D005260:Female; D008099:Liver / Q000166:cytology*; D008297:Male; D013552:Swine; D014508:Urea / Q000378:metabolism
D000418:Albumins; D003374:Coumarins; C017299:7-ethoxycoumarin; D014508:Urea; D004247:DNA; D000410:Alanine Transaminase