Production, characterization and application of monoclonal antibodies against the organophosphorus nerve agent Vx.
Two monoclonal antibodies (Vx-BB8 and Vx-EA11) to the chemical warfare agent Vx were produced and characterized. A competitive inhibition enzyme immunoassay was developed to detect Vx concentrations as low as 3.7 x 10(-7) - 3.7 x 10(-6) mol/l in biological samples. Vx-BB8 400 micrograms given intravenously immediately before 1 x LD95 Vx or 400 micrograms Vx-BB8 intraperitoneally 1.5 h-3 days before 1 x LD95 Vx could protect all the tested mice from death.
Ci
Y X
YX
Department of Chemistry, Peking University, Beijing, China.
Zhou
Y X
YX
Guo
Z Q
ZQ
Rong
K T
KT
Chang
W B
WB
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000737:chemistry / Q000627:therapeutic use*; D002619:Chemical Warfare Agents / Q000633:toxicity*; D002800:Cholinesterase Inhibitors / Q000276:immunology* / Q000633:toxicity; D005260:Female; D007116:Immunization, Passive; D007124:Immunoenzyme Techniques; D007274:Injections, Intraperitoneal; D007275:Injections, Intravenous; D051379:Mice; D008807:Mice, Inbred BALB C; D009946:Organothiophosphorus Compounds / Q000276:immunology* / Q000633:toxicity
D000911:Antibodies, Monoclonal; D002619:Chemical Warfare Agents; D002800:Cholinesterase Inhibitors; D009946:Organothiophosphorus Compounds; C009680:VX-agent