TRH receptor on immune cells: in vitro and in vivo stimulation of human lymphocyte and rat splenocyte DNA synthesis by TRH.
This work examined whether (1) immune cells express thyrotrophin releasing hormone (TRH) receptor mRNA and (2) TRH modulates lymphocyte activation. By Northern blot of RNA extracted from human peripheral blood mononuclear cells (PBMC) and rat splenocytes, a single TRH receptor mRNA band of about 3.8 kb (identical to that obtained from pituitary cells) was obtained, under both basal and stimulated conditions. A significant increase in DNA synthesis was observed in phytohemagglutinin-stimulated PBMC and concanavalin A (Con A) stimulated splenocytes when TRH (10(-6) M-10(-12) M) was added. After 5, 30, 60, 180 min and 24 h of TRH administration in vivo, a significant increase in the rat splenocyte proliferative response to Con A was observed. In vivo administration of anti-rat TSH antibody (1/1000) blocked the increase observed after 30 min of TRH administration on the Con A stimulated splenocyte response. TRH possess immunostimulatory functions directly via its receptor and indirectly via release of other immunostimulatory factors such as thyrotrophin.
Raiden
S
S
Instituto de Investigaciones Médicas, Facultad de Medicina, Universidad de Buenos Aires, Argentina.
Polack
E
E
Nahmod
V
V
Labeur
M
M
Holsboer
F
F
Arzt
E
E
D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D015152:Blotting, Northern; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis* / Q000235:genetics; D005260:Female; D015870:Gene Expression; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000187:drug effects* / Q000378:metabolism* / Q000648:ultrastructure; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D018025:Receptors, Thyrotropin-Releasing Hormone / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D013154:Spleen / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D013268:Stimulation, Chemical; D013973:Thyrotropin-Releasing Hormone / Q000276:immunology / Q000494:pharmacology*
D000906:Antibodies; D012333:RNA, Messenger; D018025:Receptors, Thyrotropin-Releasing Hormone; D013973:Thyrotropin-Releasing Hormone; D004247:DNA