Bacteriocin small of Rhizobium leguminosarum belongs to the class of N-acyl-L-homoserine lactone molecules, known as autoinducers and as quorum sensing co-transcription factors.
Small bacteriocin was isolated from the culture broth of the gram-negative bacterium Rhizobium leguminosarum, which forms symbiotic nitrogen-fixing root nodules on a number of leguminous plants. The structure of the molecule was elucidated by spectroscopic methods and identified as N-(3R-hydroxy-7-cis-tetradecanoyl)-L-homoserine lactone. The absolute configuration of both asymmetric carbon atoms in the molecule was determined by the use of the chiral solvating agents S-(+)- and R-(-)-2,2,2-trifluoro-1-(9-anthryl)-ethanol. small bacteriocin is structurally related to the quorum sensing co-transcription factors for genes from other bacteria such as Vibrio fischeri, Pseudomonas aeruginosa, Erwinia carotovora, and Agrobacterium tumefaciens which are involved in animal-microbe or plant-microbe interactions. The mechanism of regulation of such interactions by this kind of co-transcription factors is still unknown in R. leguminosarum.
Division of Pharmacognosy, Gorlaeus Laboratories, Leiden, and Gist, The Netherlands.
J
J
Schripsema
K E
KE
de Rudder
T B
TB
van Vliet
P P
PP
Lankhorst
E
E
de Vroom
J W
JW
Kijne
A A
AA
van Brussel
D015107:4-Butyrolactone / Q000031:analogs & derivatives*; D001430:Bacteriocins / Q000737:chemistry* / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy; D016961:Rhizobium leguminosarum / Q000737:chemistry*; D013237:Stereoisomerism; D014157:Transcription Factors / Q000737:chemistry*
D001430:Bacteriocins; D014157:Transcription Factors; D015107:4-Butyrolactone