Quantitative junctional permeability measurements using the confocal microscope.
This paper describes the use of a photobleach method and confocal microscopy to compare the cell-to-cell transfer rate of 5,6 carboxyfluorescein in dissociated embryonic chick lens cells with those in the anterior epithelium of the whole embryonic chick lens. The average cell-to-cell transfer rates obtained were 7.9 x 10(-3) sec-1 in the dissociated cells and 2.6 x 10(-3) sec-1 in the anterior epithelium in an intact lens.
Department of Molecular and Cellular Physiology, Stanford, CA 94305, USA.
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Miller
D000818:Animals; D002450:Cell Communication; D002463:Cell Membrane Permeability; D002642:Chick Embryo; D004848:Epithelium / Q000378:metabolism; D005452:Fluoresceins / Q000493:pharmacokinetics*; D005456:Fluorescent Dyes / Q000493:pharmacokinetics; D017629:Gap Junctions / Q000378:metabolism*; D007908:Lens, Crystalline / Q000166:cytology / Q000196:embryology / Q000378:metabolism*; D008027:Light; D018613:Microscopy, Confocal / Q000379:methods; D010777:Photochemistry
D005452:Fluoresceins; D005456:Fluorescent Dyes; C024098:6-carboxyfluorescein