Structural elucidation of the novel type VII group B Streptococcus capsular polysaccharide by high resolution NMR spectroscopy.
The type VII capsular polysaccharide isolated from the newly discovered group B Streptococcus (GBS) strain contains D-glucose, D-galactose, N-acetyl-D-glucosamine and N-acetylneuraminic acid in the molar ratio 2:2:1:1. High-resolution one- and two-dimensional (1D and 2D) 1H and 13C NMR spectroscopy of the native and desialylated polysaccharides showed the type VII GBS capsular polysaccharide to contain the following branched hexasaccharide repeating unit: [formula: see text] Despite extensive structural similarity with the previously described GBS polysaccharides, the type VII polysaccharide showed no cross-reaction with the heterologous antisera.
Institute for Biological Sciences, National Research Council of Canada, Ottawa, Ontario, Canada.
G
G
Kogan
J R
JR
Brisson
D L
DL
Kasper
C
C
von Hunolstein
G
G
Orefici
H J
HJ
Jennings
D000117:Acetylglucosamine / Q000032:analysis; D016667:Bacterial Capsules / Q000737:chemistry*; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D005690:Galactose / Q000032:analysis; D005947:Glucose / Q000032:analysis; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D019158:N-Acetylneuraminic Acid; D012091:Repetitive Sequences, Nucleic Acid; D012794:Sialic Acids / Q000032:analysis; D013292:Streptococcus agalactiae / Q000737:chemistry*
D012794:Sialic Acids; D019158:N-Acetylneuraminic Acid; D005947:Glucose; D000117:Acetylglucosamine; D005690:Galactose