Investigation of the factor VIII intron 22 repeated region (int22h) and the associated inversion junctions.
A region of intron 22 of the factor VIII gene, which contains factor VIII-associated gene A (F8A), is repeated twice more nearer the Xq telomere. It has been proposed that intrachromosomal homologous recombination occurs between the intron 22 repeat and either of the two extragenic copies, resulting in the recurrent inversions that cause almost half of all cases of severe haemophilia A. We have precisely defined the repeated region as 9.5 kb of DNA which we have termed int22h (intron 22 homologous region). The junctions of the inversions examined were shown to represent precise exchanges between the int22h repeats, thus providing conclusive evidence for homologous recombination. The three copies of int22h were compared along 8 kb of their length, using chemical mismatch analysis, and found to be 99.9% similar. The presence of such long, almost identical inverted repeats near the Xq telomere could account for the high frequency at which the inversions occur.
Division of Medical and Molecular Genetics, United Medical School of Guy's Hospital, London, UK.
J A
JA
Naylor
D
D
Buck
P
P
Green
H
H
Williamson
D
D
Bentley
F
F
Giannelli
D001483:Base Sequence; D007446:Chromosome Inversion; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D005169:Factor VIII / Q000235:genetics* / Q000378:metabolism; D006467:Hemophilia A / Q000235:genetics; D006801:Humans; D007438:Introns; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011995:Recombination, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D017422:Sequence Analysis, DNA
D005169:Factor VIII; D015252:Deoxyribonucleases, Type II Site-Specific