Haemolytic activity of Actinobacillus actinomycetemcomitans strains on different blood types.
Haemolytic activity of sixty nine Actinobacillus actinomycetemcomitans strains on different animal and human blood types was examined by using a trypticase soy agar supplemented with yeast extract (0.5%). Blood types used were: rabbit, sheep and human (A, Rh+; A, Rh-; B, Rh+; B, Rh-; O, Rh+; O, Rh-; AB, Rh+; AB, Rh- groups). Plates were inoculated and, incubated in microaerophilic conditions, at 37 degrees C, for 48 h. The haemolytic activity of the tested strains was characterized as alpha-haemolysis. Only two isolates were not haemolytic on all blood types (2.9%), two strains were haemolytic only on human blood (one strain on AB, Rh+ group and another one on A, Rh+ and AB, Rh+ groups). No specificity between haemolysin produced by the tested strains and blood type was observed.
Departamento de Microbiologia, Universidade de São Paulo, Brazil.
M J
MJ
Avila-Campos
D016976:Aggregatibacter actinomycetemcomitans / Q000472:pathogenicity / Q000502:physiology*; D000818:Animals; D001789:Blood Group Antigens; D006460:Hemolysin Proteins / Q000096:biosynthesis; D006461:Hemolysis; D006801:Humans; D010510:Periodontal Diseases / Q000097:blood / Q000382:microbiology*; D010641:Phenotype; D011817:Rabbits; D051381:Rats
D001789:Blood Group Antigens; D006460:Hemolysin Proteins