Human adenovirus serotype 3 (Ad3) and the Ad3 fiber protein bind to a 130-kDa membrane protein on HeLa cells.
The fiber protein of adenovirus mediates the interaction of adenovirus with cell membrane receptors. We have produced the Ad3 fiber protein in the baculovirus expression system. Biochemical, morphological and functional analyses showed that the recombinant fiber was properly folded and functionally competent. The specific binding of Ad3 virus to two HeLa membrane proteins of 130 and 100 kDa was demonstrated with an overlay protein binding assay. In the same assay, Ad3 fiber only recognized the 130-kDa protein. Divalent cations seemed to be important for the interaction of both virus and fiber with these proteins.
Di Guilmi
A M
AM
Institute de Biologie Structurale, Grenoble, France.
Barge
A
A
Kitts
P
P
Gout
E
E
Chroboczek
J
J
D000260:Adenoviruses, Human / Q000145:classification / Q000378:metabolism*; D002213:Capsid / Q000378:metabolism* / Q000648:ultrastructure; D036022:Capsid Proteins; D002413:Cations, Divalent; D002462:Cell Membrane / Q000821:virology; D002918:Chymotrypsin; D006367:HeLa Cells; D006801:Humans; D011991:Receptors, Virus / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012703:Serotyping; D014357:Trypsin
D036022:Capsid Proteins; D002413:Cations, Divalent; D011991:Receptors, Virus; D011993:Recombinant Fusion Proteins; C018315:hexon capsid protein, Adenovirus; D002918:Chymotrypsin; D014357:Trypsin