Characterisation of an Arabidopsis thaliana cDNA encoding glutathione synthetase.
An Arabidopsis thaliana cDNA (AtGSHS) encoding a protein with high primary sequence identity to cDNAs previously isolated from Xenopus laevis (42%), Schizosaccharomyces pombe (40%), Rattus norvegicus (40%) and Homo sapiens (37%) encoding glutathione synthetase (EC 6.3.2.3) has been isolated by functional complementation of an Escherichia coli mutant deficient in this enzyme. AtGSHS is encoded by a single gene, GSHB, as determined by Southern blot analysis and the corresponding mRNA is abundant in both roots and leaves of Arabidopsis.
Rawlins
M R
MR
Department of Plant Sciences, University of Oxford, UK.
Leaver
C J
CJ
May
M J
MJ
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D017360:Arabidopsis / Q000235:genetics*; D001483:Base Sequence; D015139:Blotting, Southern; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics; D005978:Glutathione / Q000096:biosynthesis / Q000378:metabolism; D005981:Glutathione Synthase / Q000172:deficiency / Q000235:genetics*; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D051381:Rats; D017421:Sequence Analysis; D017386:Sequence Homology, Amino Acid
D000596:Amino Acids; D018076:DNA, Complementary; D012333:RNA, Messenger; D005981:Glutathione Synthase; D005978:Glutathione