Triglyceride uptake in muscles in rats.
Exogenous lipid is assimilated with different priorities in adipose tissue regions and varies in the fasting and fed conditions. The quantitative role of uptake of lipid in muscle has not been evaluated. In order to examine the uptake in other than adipose tissues, U14C-oleic acid in sesame oil was administered orally to conscious rats, and lipid label measured after different times in serum, heart, liver, mesenteric, retroperitoneal, inguinal and epididymal fat pads, as well as in red and white parts of gastrocnemius, extensor digitorum longus and soleus muscles. Lipid uptake in total adipose tissue was calculated from dissected adipose tissues plus lipids extracted from the eviscerated, skinned carcass. Lipid uptake in total muscle tissue was estimated from label in dissected muscles plus that in the carcass, assuming similar intracellular lipid contents and radioactivity as that averaged from dissected muscles. Lipid uptake in the liver was calculated from directly extracted lipid. Four hours after lipid administration to fed rats lipid radioactivity in heart and serum was minimal and had essentially disappeared at 8 hours. Liver label declined rapidly from peak values at or before 4 hours. Adipose tissue radioactivity increased gradually up to 16 hours and then decreased. Label in muscles was highest at 4 hours in the red gastrocnemius, and then decreased, while the other muscles showed a constant radioactivity over the observation period (24 hours). Radioactivity expressed per unit muscle mass seemed to be proportional to the oxidative capacity of muscles.(ABSTRACT TRUNCATED AT 250 WORDS)
Wallenberg Laboratory, Sahlgren's Hospital, University of Göteborg, Sweden.
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D000273:Adipose Tissue; D000818:Animals; D002250:Carbon Radioisotopes; D005215:Fasting; D005502:Food; D007700:Kinetics; D008099:Liver / Q000378:metabolism; D008297:Male; D018482:Muscle, Skeletal / Q000378:metabolism*; D019301:Oleic Acid; D009829:Oleic Acids / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012715:Sesame Oil; D014280:Triglycerides / Q000378:metabolism*
D002250:Carbon Radioisotopes; D009829:Oleic Acids; D014280:Triglycerides; D019301:Oleic Acid; D012715:Sesame Oil