Influence of carbon and nitrogen sources on glutathione catabolic enzymes in Candida albicans during dimorphism.
The effect of carbon sources, glucose and sucrose, and nitrogen sources such as ammonia, glutamate and L-citrulline on the activities of glutathione metabolic enzymes has been studied. Yeast and mycelial cells were used to identify changes in activity levels of glutathione reductase (GSSGR), glutathione transferase (GST), glutathione peroxidase (GPX) and gamma-glutamyl transpeptidase (GGT). Enzyme activities from cells grown in sucrose media were lower than in glucose media regardless of the enzyme tested, morphological form, or the growth interval. In all enzymes except GST, activity was higher in yeast form than in mycelia, regardless of nitrogen source, with lower activity from 24 to 72 h than at 96 h. In citrulline media, yeast form showed the maximum GST, GGT, and GPX activity. In ammonia-amended media, mycelia showed maximum activity in GGT, whereas in glutamate media, mycelia showed the maximum activity in GST. Also, the type of nitrogen source had no effect on GPX activity in the mycelial form. Finally, changing the nitrogen source showed no significant effect on GSSGR activity, either in the yeast or mycelial form.
Gunasekaran
S
S
Department of Biology, Fisk University, Nashville, TN 37208, USA.
Imbayagwo
M
M
McDonald
L
L
Gunasekaran
M
M
Manavathu
E
E
D000641:Ammonia / Q000378:metabolism; D002176:Candida albicans / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism; D002956:Citrulline / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D018698:Glutamic Acid / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism*; D005979:Glutathione Peroxidase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D013395:Sucrose / Q000378:metabolism; D005723:gamma-Glutamyltransferase / Q000378:metabolism
D002956:Citrulline; D018698:Glutamic Acid; D013395:Sucrose; D000641:Ammonia; D005979:Glutathione Peroxidase; D005980:Glutathione Reductase; D005723:gamma-Glutamyltransferase; D005982:Glutathione Transferase; D005978:Glutathione; D005947:Glucose