Differential accumulation of the transcripts of 22 novel protein kinase genes in Arabidopsis thaliana.
22 novel members of the Arabidopsis thaliana protein kinase family (AKs) were identified by using degenerate oligonucleotide primers directed to highly conserved amino acid sequences of the protein kinase (PK) catalytic domain. Of these 22 genes, 16 turned out to carry intron sequences. Homologies of AK sequences were detected to S-locus receptor protein kinases (SRKs) from Brassica spp., to SRK-like PKs from maize and A. thaliana and to several other receptor PKs from A. thaliana. Sequence similarity was also detected to Ca(2+)-dependent PKs (CDPKs) from rape and soybean, to SNF1 and to CDC2 homologues. The genomic organization and the accumulation of the mRNAs from these 22 AK genes were investigated.
Botanisches Institut, Universität München, Germany.
F
F
Thümmler
M
M
Kirchner
R
R
Teuber
P
P
Dittrich
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics; D001483:Base Sequence; D001665:Binding Sites; D015139:Blotting, Southern; D017931:DNA Primers; D018744:DNA, Plant / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D015870:Gene Expression; D017343:Genes, Plant; D007438:Introns; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011494:Protein Kinases / Q000096:biosynthesis*; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic
D017931:DNA Primers; D018744:DNA, Plant; D011494:Protein Kinases