Protracted, relapsing and demyelinating experimental autoimmune encephalomyelitis in DA rats immunized with syngeneic spinal cord and incomplete Freund's adjuvant.
Experimental autoimmune encephalomyelitis (EAE) is a model for multiple sclerosis (MS). However, MS is a chronic, relapsing and demyelinating disease, whereas EAE in rats is typically a brief and monophasic disorder showing little demyelination. We demonstrate here that DA rats develop severe, protracted and relapsing EAE (SPR-EAE) after a subcutaneous immunization at the tail base with syngeneic spinal cord and incomplete Freund's adjuvant (IFA). The neurological deficits were accompanied by demyelinating inflammatory lesions in the spinal cord, with infiltrating T lymphocytes and perivascular deposition of immunoglobulins and complement. The induction of SPR-EAE was associated with humoral autoreactivity to myelin oligodendrocyte glycoprotein (MOG) and cellular autoreactivity to the rat myelin basic protein (MBP) peptides 69-87 and 87-101. These two peptides, as well as whole rat MBP, were encephalitogenic. In conclusion, we believe that the presently described demyelinating SPR-EAE represents a useful model for MS.
Department of Rheumatology; Karolinska Hospital, Karolinska Institute, Stockholm, Sweden.
J C
JC
Lorentzen
S
S
Issazadeh
M
M
Storch
M I
MI
Mustafa
H
H
Lassman
C
C
Linington
L
L
Klareskog
T
T
Olsson
D000595:Amino Acid Sequence; D000818:Animals; D001323:Autoantibodies / Q000096:biosynthesis; D003711:Demyelinating Diseases / Q000276:immunology*; D004195:Disease Models, Animal; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology*; D005620:Freund's Adjuvant / Q000494:pharmacology*; D006168:Guinea Pigs; D007114:Immunization; D007150:Immunohistochemistry; D007279:Injections, Subcutaneous; D008969:Molecular Sequence Data; D004676:Myelin Basic Protein / Q000494:pharmacology; D009185:Myelin Proteins; D018992:Myelin-Associated Glycoprotein / Q000494:pharmacology; D063308:Myelin-Oligodendrocyte Glycoprotein; D010455:Peptides / Q000494:pharmacology; D010641:Phenotype; D051381:Rats; D011919:Rats, Inbred Strains; D012008:Recurrence; D015203:Reproducibility of Results; D013116:Spinal Cord / Q000276:immunology* / Q000473:pathology; D013997:Time Factors
D001323:Autoantibodies; C571328:Mog protein, rat; D004676:Myelin Basic Protein; D009185:Myelin Proteins; D018992:Myelin-Associated Glycoprotein; D063308:Myelin-Oligodendrocyte Glycoprotein; D010455:Peptides; D005620:Freund's Adjuvant