Open reading frame 176 in the photosynthesis gene cluster of Rhodobacter capsulatus encodes idi, a gene for isopentenyl diphosphate isomerase.
Isopentenyl diphosphate (IPP) isomerase catalyzes an essential activation step in the isoprenoid biosynthetic pathway. A database search based on probes from the highly conserved regions in three eukaryotic IPP isomerases revealed substantial similarity with ORF176 in the photosynthesis gene cluster in Rhodobacter capsulatus. The open reading frame was cloned into an Escherichia coli expression vector. The encoded 20-kDa protein, which was purified in two steps by ion exchange and hydrophobic interaction chromatography, catalyzed the interconversion of IPP and dimethylallyl diphosphate. Thus, the photosynthesis gene cluster encodes all of the enzymes required to incorporate IPP into the ultimate carotenoid and bacteriochlorophyll metabolites in R. capsulatus. More recent searches uncovered additional putative open reading frames for IPP isomerase in seed-bearing plants (Oryza sativa, Arabidopsis thaliana, and Clarkia breweri), a worm (Caenorhabditis elegans), and another eubacterium (Escherichia coli). The R. capsulatus enzyme is the smallest of the IPP isomerases to be identified thus far and may consist mostly of a fundamental catalytic core for the enzyme.
Hahn
F M
FM
Department of Chemistry, University of Utah, Salt Lake City 84112, USA.
Baker
J A
JA
Poulter
C D
CD
D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D019748:Carbon-Carbon Double Bond Isomerases; D005798:Genes, Bacterial; D045782:Hemiterpenes; D007535:Isomerases / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D005810:Multigene Family; D016366:Open Reading Frames; D016354:Rhodobacter capsulatus / Q000235:genetics*
D045782:Hemiterpenes; D007535:Isomerases; D019748:Carbon-Carbon Double Bond Isomerases; C021453:isopentenyldiphosphate delta-isomerase