Tyrosine 75 on the flap contributes to enhance catalytic efficiency of a fungal aspartic proteinase, Mucor pusillus pepsin.
Beppu
T
T
Department of Agricultural Chemistry, Faculty of Agriculture, University of Tokyo, Japan.
Park
Y N
YN
Aikawa
J
J
Nishiyama
M
M
Horinouchi
S
S
D000818:Animals; D002384:Catalysis; D002942:Circular Dichroism; D003001:Cloning, Molecular; D005800:Genes, Fungal; D006031:Glycosylation; D007700:Kinetics; D008892:Milk; D008969:Molecular Sequence Data; D009089:Mucor / Q000201:enzymology* / Q000235:genetics; D016297:Mutagenesis, Site-Directed; D010434:Pepsin A / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D017354:Point Mutation; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D012441:Saccharomyces cerevisiae; D014443:Tyrosine
D011994:Recombinant Proteins; D014443:Tyrosine; D010434:Pepsin A