Tobacco MAP kinase: a possible mediator in wound signal transduction pathways.
A complementary DNA encoding a mitogen-activated protein (MAP) kinase homolog has been isolated from tobacco plants. Transcripts of the corresponding gene were not observed in healthy tobacco leaves but began to accumulate 1 minute after mechanical wounding. In tobacco plants transformed with the cloned complementary DNA, trans inactivation of the endogenous homologous gene occurred, and both production of wound-induced jasmonic acid and accumulation of wound-inducible gene transcripts were inhibited. In contrast, the levels of salicylic acid and transcripts for pathogen-inducible, acidic pathogenesis-related proteins were increased upon wounding. These results indicate that this MAP kinase is part of the initial response of higher plants to mechanical wounding.
Seo
S
S
Institute of Applied Biochemistry, University of Tsukuba, Ibaraki, Japan.
Okamoto
M
M
Seto
H
H
Ishizuka
K
K
Sano
H
H
Ohashi
Y
Y
D000595:Amino Acid Sequence; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D003517:Cyclopentanes / Q000378:metabolism; D018076:DNA, Complementary / Q000235:genetics; D017343:Genes, Plant; D008969:Molecular Sequence Data; D054883:Oxylipins; D010741:Phospholipases A / Q000378:metabolism; D010766:Phosphorylation; D010940:Plant Proteins / Q000378:metabolism; D030821:Plants, Genetically Modified; D010947:Plants, Toxic; D012459:Salicylates / Q000378:metabolism; D020156:Salicylic Acid; D015398:Signal Transduction; D014026:Nicotiana / Q000201:enzymology* / Q000235:genetics
D003517:Cyclopentanes; D018076:DNA, Complementary; D054883:Oxylipins; D010940:Plant Proteins; D012459:Salicylates; C053376:pathogenesis-related proteins, plant; C011006:jasmonic acid; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D010741:Phospholipases A; D020156:Salicylic Acid