22-kDa and 20-kDa hGH isoforms show differential effects when assayed in 3T3-F442A and 3T3-F442A/C4 adipocytes.
To known whether 22.0-kDa human growth hormone (hGH22K) and the 20.0-kDa isoform (hGH20K) show different activities, we used 3T3-F442A and 3T3-F442A/C4 cells to evaluate their adipogenic and metabolic effects. Both isoforms had similar adipogenic and insulin-like activities. Lipolytic and diabetogenic effects of hGH22K were, respectively, 12.5 and 1.7-fold higher than those found for hGH20K. The 3T3-F442A/C4 clone was not responsive to insulin-like and diabetogenic effects of hGH. The results suggest that adipogenic and lipolytic effects of hGH are mediated by mechanisms different from those involved in insulin-like and diabetogenic activities.
Juárez-Aguilar
E
E
Dept. of Cell Biology, Centro de Investigación y de Estudios Avanzados del I.P.N, México D.F. Mexico.
Castro-Muñozledo
F
F
D016475:3T3 Cells; D000273:Adipose Tissue / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D005947:Glucose / Q000378:metabolism; D013006:Growth Hormone / Q000737:chemistry / Q000235:genetics / Q000494:pharmacology*; D006801:Humans; D007328:Insulin / Q000494:pharmacology; D008055:Lipids / Q000096:biosynthesis; D008066:Lipolysis / Q000187:drug effects; D051379:Mice; D008970:Molecular Weight; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000494:pharmacology
D007328:Insulin; D008055:Lipids; D011994:Recombinant Proteins; D013006:Growth Hormone; D005947:Glucose