Lipoprotein(a) stimulates the proliferation of cultured human arterial smooth muscle cells through two pathways.
We investigated the effect of lipoprotein(a) (Lp(a)) on proliferation of human arterial smooth muscle cells (SMCs) and its mechanisms of action. Low density lipoprotein (LDL), Lp(a) and apolipoprotein(a) (apo(a)) significantly stimulated the proliferation of SMCs. Lp(a) and apo(a) reduced the amount of active transforming growth factor-beta (TGF-beta) with the mink lung epithelial cell bioassay, however LDL had no effect. Lp(a), but not apo(a), significantly stimulated the proliferation of SMCs even in the presence of a neutralizing antibody for TGF-beta. Our results suggest that Lp(a) stimulates the proliferation of SMCs via apo(a)-induced inhibition of TGF-beta activation and stimulation of SMCs by the LDL-particle of Lp(a).
First Department of Internal Medicine, Faculty of Medicine, Kagoshima University, Japan.
M
M
Miyata
S
S
Biro
H
H
Kaieda
H
H
Tanaka
D001054:Apolipoproteins A / Q000494:pharmacology; D001158:Arteries / Q000166:cytology*; D002455:Cell Division; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D006801:Humans; D017270:Lipoprotein(a) / Q000494:pharmacology*; D008077:Lipoproteins, LDL / Q000494:pharmacology; D024510:Muscle Development; D009131:Muscle, Smooth, Vascular / Q000187:drug effects* / Q000254:growth & development; D016212:Transforming Growth Factor beta / Q000378:metabolism*
D001054:Apolipoproteins A; D017270:Lipoprotein(a); D008077:Lipoproteins, LDL; D016212:Transforming Growth Factor beta