Stabilization of the tight junction of the intestinal Caco-2 cell monolayer by milk whey proteins.
The tight junction (TJ) of the human intestinal Caco-2-SF monolayer was shown not to have been stably formed. TJ was stabilized by incubating the cell monolayer with beta-lactoglobulin (beta-Lg) and bovine serum albumin (BSA). Thus, the Caco-2-SF cell-culture system will provide a useful model for studying the factors which stabilize TJ and for investigating the mechanism of TJ regulation.
School of Food and Nutritional Sciences, University of Shizuoka, Japan.
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K
Hashimoto
K
K
Takeda
T
T
Nakayama
M
M
Shimizu
D000818:Animals; D018938:Caco-2 Cells; D002417:Cattle; D002463:Cell Membrane Permeability / Q000187:drug effects; D006801:Humans; D007413:Intestinal Mucosa / Q000166:cytology; D007782:Lactoglobulins / Q000378:metabolism / Q000494:pharmacology; D008892:Milk; D008894:Milk Proteins / Q000494:pharmacology*; D012710:Serum Albumin, Bovine / Q000378:metabolism / Q000494:pharmacology; D019108:Tight Junctions / Q000187:drug effects* / Q000378:metabolism; D014357:Trypsin / Q000378:metabolism; D000067816:Whey Proteins
D007782:Lactoglobulins; D008894:Milk Proteins; D000067816:Whey Proteins; D012710:Serum Albumin, Bovine; D014357:Trypsin