Differential regulation of the murine ribosomal protein L26 gene in macrophage activation.
In mouse RAW 264.7 macrophages, the gene for ribosomal protein L26 is positively regulated by silica. In order to study L26 gene expression a near full-length cDNA for mouse L26 was isolated and characterized. Sequence analysis revealed that mouse L26 is a 145 amino acid protein highly homologous to other vertebrate L26 proteins. The treatment of RAW 264.7 cells with the inflammatory mediators LPS and IFN gamma induced the expression of L26 mRNA, but the patterns of expression obtained differed markedly from silica. On the contrary, TNF alpha acted as a down-regulator of L26 gene. Our results suggest that the synthesis of ribosomal components in response to macrophage activators is inducer-specific. Mouse genomic DNA analysis revealed the presence of multiple (10-12) sequences related to the L26 gene.
Departamento de Biología Funcional, Universidad de Oviedo, Spain.
F
F
Segade
E
E
Claudio
B
B
Hurlé
S
S
Ramos
P S
PS
Lazo
D000595:Amino Acid Sequence; D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology; D001427:Bacterial Toxins / Q000494:pharmacology; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D002460:Cell Line; D002478:Cells, Cultured; D004247:DNA / Q000032:analysis / Q000737:chemistry; D017931:DNA Primers / Q000737:chemistry; D004731:Endotoxins / Q000494:pharmacology; D005786:Gene Expression Regulation; D007371:Interferon-gamma / Q000494:pharmacology; D008262:Macrophage Activation / Q000187:drug effects*; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000096:biosynthesis; D012269:Ribosomal Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D012475:Salmonella; D012822:Silicon Dioxide / Q000494:pharmacology
D000998:Antiviral Agents; D001427:Bacterial Toxins; D017931:DNA Primers; D004731:Endotoxins; D012333:RNA, Messenger; D012269:Ribosomal Proteins; C027952:salmonella toxin; C060480:Rpl26 protein, mouse; D012822:Silicon Dioxide; D007371:Interferon-gamma; D004247:DNA