Biotransformation of corticosteroids by Penicillium decumbens ATCC 10436.
The biotransformation of a series of corticosteroids by the fungus Penicillium decumbens ATCC 10436 has been investigated. Conversion to the corresponding 5 alpha-dihydrosteroid was observed for all the delta 4-3-ketosteroids studied with the exception of deoxycorticosterone, which was converted to a delta 1.4-diene. Deoxycorticosterone acetate was, however, converted to a 5 alpha-dihydro product concomitant with ester hydrolysis. Other substrates carrying a C-21 acetoxy group were also hydrolyzed to the alcohol. In two cases (resulting from deoxycorticosterone acetate and 11-deoxycortisone) the 5 alpha-3-keto product was further reduced to the 3 beta-alcohol. No reduction of delta 1.4-dienes was observed.
Holland
H L
HL
Department of Chemistry, Brock University, St. Catharines, Ontario, Canada.
Nguyen
D H
DH
Pearson
N M
NM
D013741:3-Oxo-5-alpha-Steroid 4-Dehydrogenase / Q000378:metabolism; D000305:Adrenal Cortex Hormones / Q000737:chemistry / Q000378:metabolism*; D001711:Biotransformation; D002855:Chromatography, Thin Layer; D007664:Ketosteroids / Q000737:chemistry / Q000378:metabolism; D013058:Mass Spectrometry; D010407:Penicillium / Q000378:metabolism*; D011374:Progesterone / Q000378:metabolism
D000305:Adrenal Cortex Hormones; D007664:Ketosteroids; D011374:Progesterone; D013741:3-Oxo-5-alpha-Steroid 4-Dehydrogenase