Synthesis of novel oligosaccharides from leucrose by an alpha-glucosidase.
An alpha-glucosidase was purified in an electrophoretically pure state from an extract of koji culture of Aspergillus sp. KT-11. This enzyme was found to have a transferring activity when the reaction was done in a high concentration of leucrose at pH 4.5. Two kinds of transfer products, fractions I and II, were obtained from leucrose by the enzyme and they were identified as [(alpha-D-glucopyranosyl-(1-->6)-alpha-D-glucopyranosyl-(1-->6)-alpha-D- glucopyranosyl-(1-->5)-D-fructopyranose] and [alpha-D-glucopyranosyl-(1-->6)-alpha-D-glucopyranosyl-(1-->5)-D- fructopyranose], respectively. These are considered to be novel oligosaccharides.
Anindyawati
T
T
Faculty of Science, Osaka City University, Japan.
Yamaguchi
H
H
Furuichi
K
K
Iizuka
M
M
Minamiura
N
N
D001230:Aspergillus / Q000201:enzymology; D002240:Carbohydrate Sequence; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D002855:Chromatography, Thin Layer; D004187:Disaccharides / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000096:biosynthesis* / Q000737:chemistry; D000520:alpha-Glucosidases / Q000302:isolation & purification / Q000378:metabolism*
D004187:Disaccharides; D009844:Oligosaccharides; D000520:alpha-Glucosidases; C060525:leucrose