[The frequency of generalized infection in adult fasting ticks of the genus Ixodes in foci of borreliosis in Russia and the USA].
A total of 740 adult Ixodes persulcatus ticks were collected from the vegetation by flagging in Russian foci where Borrelia afzelii and B. garinii circulate, and 156 I. dammini ticks were collected in northwestern USA regions in foci with B. burgdorferi s.str. circulation. Smears prepared from the internal organs of ticks were stained according to Romanovsky-Giemsa and analyzed under a microscope at a x 1125 magnification. All borreliae in 250 microscopic fields were counted, and concentration of microbial bodies per 100 microscopic fields was determined. The general level of infection by Borrelia in both vectors was similar: 26.2 x 3.2 in I. persulcatus and 26.3 +/- 7 in I. dammini. However, the proportions of ticks with generalized infections differ considerably (12.9 +/- 4.8 in I. persulcatus compared with 2.4 +/- 4.8 in I. dammini; significance of difference t = 3.1). We did not reveal any definite increase in the proportion of ticks with borreliae in the salivary glands among ticks with high concentrations of microbial bodies in the gut. In 25 I. persulcatus ticks with generalized infections, series of actual numbers of borreliae (per 100 microscopic fields) found in the gut and salivary glands did not correlate with one another (r = -0.23). These results confirm our previous conclusion (Korenberg, 1994) that frequencies of generalized infection in main vectors of different ixodid tick-borne borrelioses are also different, which is probably due to peculiarities of relationships between spirochetes of each species and corresponding tick vectors. These factors can be responsible for differences in the ways of horizontal and vertical transmission of pathogens belonging to the group under study.
Moskvitina
G G
GG
Korenberg
E I
EI
Spielman
A
A
Shchegoleva
T V
TV
D000818:Animals; D001096:Arachnid Vectors / Q000382:microbiology*; D001898:Borrelia / Q000302:isolation & purification; D001899:Borrelia Infections / Q000382:microbiology / Q000635:transmission*; D004197:Disease Reservoirs; D005247:Feeding Behavior; D007422:Intestines / Q000382:microbiology; D018884:Ixodes / Q000382:microbiology*; D012426:Russia; D012469:Salivary Glands / Q000382:microbiology; D013045:Species Specificity; D014481:United States
O chastote generalizovannoĭ infektsii u vzroslykh golodnykh kleshscheĭ roda Ixodes v ochagakh borreliozov Rossii i SShA.