Dissecting the loci controlling fetal haemoglobin production on chromosomes 11p and 6q by the regressive approach.
The changes in the type of haemoglobin (Hb) produced during embryonic, fetal and adult life, have served as a paradigm for understanding the developmental regulation of human genes. A genetically determined persistence of fetal Hb synthesis has an ameliorating effect on beta thalassaemia and sickle cell anaemia, globally the commonest single gene disorders. The search for the putative gene(s) controlling the level of fetal Hb production has been extremely difficult because this trait may be influenced by several factors. We have studied a large kindred with hereditary persistence of fetal haemoglobin (HPFH). Using a genetic mapping strategy and statistical methods that account simultaneously for the effects of several genetic factors, we have demonstrated that in addition to the two factors (beta thalassaemia and Xmn I-G gamma site) on chromosome 11p, there is a third major genetic determinant for fetal Hb production localized on chromosome 6q.
MRC Molecular Haematology Unit, John Radcliffe Hospital, Oxford, UK.
J E
JE
Craig
J
J
Rochette
C A
CA
Fisher
D J
DJ
Weatherall
S
S
Marc
G M
GM
Lathrop
F
F
Demenais
S
S
Thein
D002880:Chromosomes, Human, Pair 11; D002896:Chromosomes, Human, Pair 6; D015252:Deoxyribonucleases, Type II Site-Specific; D005260:Female; D005319:Fetal Hemoglobin / Q000235:genetics*; D005786:Gene Expression Regulation; D008040:Genetic Linkage; D005819:Genetic Markers; D006453:Hemoglobinopathies / Q000235:genetics*; D006801:Humans; D008297:Male; D012150:Polymorphism, Restriction Fragment Length; D012044:Regression Analysis; D017086:beta-Thalassemia / Q000235:genetics
D005819:Genetic Markers; D005319:Fetal Hemoglobin; C028649:endodeoxyribonuclease XmnI; D015252:Deoxyribonucleases, Type II Site-Specific