Regulation of PHO4 nuclear localization by the PHO80-PHO85 cyclin-CDK complex.
PHO4, a transcription factor required for induction of the PHO5 gene in response to phosphate starvation, is phosphorylated by the PHO80-PHO85 cyclin-CDK (cyclin-dependent kinase) complex when yeast are grown in phosphate-rich medium. PHO4 was shown to be concentrated in the nucleus when yeast were starved for phosphate and was predominantly cytoplasmic when yeast were grown in phosphate-rich medium. The sites of phosphorylation on PHO4 were identified, and phosphorylation was shown to be required for full repression of PHO5 transcription when yeast were grown in high phosphate. Thus, phosphorylation of PHO4 by PHO80-PHO85 turns off PHO5 transcription by regulating the nuclear localization of PHO4.
Department of Biochemistry and Biophysics, University of California at San Francisco, School of Medicine 94143-0448, USA.
E M
EM
O'Neill
A
A
Kaffman
E R
ER
Jolly
E K
EK
O'Shea
D000595:Amino Acid Sequence; D002467:Cell Nucleus / Q000378:metabolism*; D003470:Culture Media; D018844:Cyclin-Dependent Kinases / Q000378:metabolism*; D016213:Cyclins / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism; D004268:DNA-Binding Proteins; D004151:Dipeptides / Q000378:metabolism; D005656:Fungal Proteins / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal; D026901:Membrane Transport Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D009154:Mutation; D028061:Phosphate Transport Proteins; D010710:Phosphates / Q000378:metabolism; D010766:Phosphorylation; D012097:Repressor Proteins; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors / Q000378:metabolism*
D003470:Culture Media; D016213:Cyclins; D004268:DNA-Binding Proteins; D004151:Dipeptides; D005656:Fungal Proteins; D026901:Membrane Transport Proteins; C060292:PHO4 protein, S cerevisiae; C066824:PHO80 protein, S cerevisiae; D028061:Phosphate Transport Proteins; D010710:Phosphates; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; C055831:phosphate permease; D018844:Cyclin-Dependent Kinases; C058605:PHO85 protein, S cerevisiae