Expression of cDNA for a bark lectin of Robinia in transgenic tobacco plants.
A cDNA encoding a bark lectin of Robinia pseudoacacia was introduced into tobacco plants. The expression of the lectin cDNA under control of the 35S promoter was confirmed by Western blot analysis and a hemagglutination assay of extracts of transgenic plants. Western blot analysis revealed that the subunit of the lectin from tobacco had a molecular mass of 29 kDa. The sequence of nine amino acids from the N-terminus of the lectin from transgenic tobacco plants was identical to that of the bark lectin from Robinia, indicating that the lectin had been processed correctly at its N-terminus in tobacco. The molecular mass of the purified native lectin produced by tobacco plants was estimated to be 112 kDa by gel filtration on a column of Superdex 200. It is suggested that the lectin subunits assembled to form tetramers in transgenic tobacco plants.
Forestry and Forest Products Research Institute, Ibaraki, Japan.
K
K
Tazaki
K
K
Yoshida
K
K
Shinohara
T
T
Koshiba
N
N
Yamamoto
D000595:Amino Acid Sequence; D015153:Blotting, Western; D002850:Chromatography, Gel; D018076:DNA, Complementary / Q000378:metabolism*; D015870:Gene Expression; D018014:Gene Transfer Techniques; D006386:Hemagglutination Tests; D037102:Lectins / Q000032:analysis / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010446:Peptide Fragments / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry; D037121:Plant Lectins; D030821:Plants, Genetically Modified; D010947:Plants, Toxic; D011401:Promoter Regions, Genetic; D014026:Nicotiana / Q000235:genetics*
D018076:DNA, Complementary; D037102:Lectins; D010446:Peptide Fragments; D037121:Plant Lectins; C047720:Robinia lectin