Susceptibility of HIV to inactivation by disinfectants and ultraviolet light.
Assays were developed to assess a variety of conditions and presentations of infectious HIV to potential inactivating sources. A range of commercially available disinfectants with active constituents including glutaraldehyde, chlorine, phenolics, alcohol, iodine and quaternary ammonium compounds was tested. In addition, u.v. light was investigated as a potential inactivating source. All products were assessed against cell-free HIV in culture medium and cell-associated HIV suspended in medium or whole human blood. All products completely inactivated cell-free HIV following a 1 min exposure. However, cell-associated HIV was more resilient, requiring exposure of 5 min or more for some disinfectants. The effectiveness of the disinfectants was further compromised in the presence of blood.
Victorian Infectious Diseases Reference Laboratory, Fairfield Hospital, Fairfield, Australia.
J D
JD
Druce
D
D
Jardine
S A
SA
Locarnini
C J
CJ
Birch
D001769:Blood / Q000821:virology; D002460:Cell Line; D003470:Culture Media; D004202:Disinfectants / Q000494:pharmacology*; D004203:Disinfection / Q000379:methods*; D015497:HIV-1 / Q000187:drug effects* / Q000528:radiation effects; D006801:Humans; D014466:Ultraviolet Rays
D003470:Culture Media; D004202:Disinfectants