Microwave induced alteration in the neuron specific enolase gene expression.
Exposure of pNGE7, a recombinant clone containing the coding and regulatory sequences for the expression of neuron specific enolase gene, cells to electromagnetic radiations (915 MHz, 16 Hz AM, SAR 0.05 mW/kg) resulted in the elevation of neuron specific enolase (NSE), a diagnostic marker for neuron and lung cancer. Using ion-exchange chromatography we separated the neuron specific enolase activity from the non-neuronal enolase (NNE) activity and observed an alteration in the expression of neuron specific enolase and non-neuronal enolase. The clinical applications of the present studies have been discussed.
Department of Biology, Howard University, Washington, DC 20059, USA.
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D001483:Base Sequence; D014408:Biomarkers, Tumor / Q000032:analysis*; D002852:Chromatography, Ion Exchange; D004790:Enzyme Induction / Q000528:radiation effects; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D006801:Humans; D008872:Microwaves; D008969:Molecular Sequence Data; D010751:Phosphopyruvate Hydratase / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification
D014408:Biomarkers, Tumor; D011994:Recombinant Proteins; D010751:Phosphopyruvate Hydratase