Expression of p53 and coexistence of HPV in premalignant lesions and in cervical cancer.
Fifty-four paraffin embedded tissue sections from patients with dysplasia (21 cases) and with cervical cancer (33 cases) were analysed. HPV was detected and identified in two stages. Firstly, using mixed starters, chosen genomic DNA sequences were amplified; secondly the material thus obtained was analyzed by hybridization method using oligonucleotyde 31-P labelled probe. HPVs of type 6, 11, 16, 18, 33 were identified. The p-53 expression was assayed by immunohistochemical method. HPV infection was often associated with dysplasia and cervical cancer. In cervical cancer mainly HPV 16 and 18 with high oncogenic potential were found. The p-53 was present rarely, and in minute quantities. No correlation was observed between presence of p-53 and HPVs DNA.
Markowska
J
J
Department of Gynecology, Medical School, Poznan Center of Oncology, Poland.
Nowak
M
M
Bar
M
M
Harlozinska
A
A
D015344:DNA Probes, HPV; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D027383:Papillomaviridae / Q000302:isolation & purification*; D016159:Tumor Suppressor Protein p53 / Q000032:analysis*; D002578:Uterine Cervical Dysplasia / Q000737:chemistry* / Q000473:pathology / Q000821:virology*; D002583:Uterine Cervical Neoplasms / Q000737:chemistry* / Q000473:pathology / Q000821:virology*
D015344:DNA Probes, HPV; D004279:DNA, Viral; D016159:Tumor Suppressor Protein p53