In situ localization of nucleolin in the plant nucleolar matrix.
The analysis of isolated nucleolar matrices from onion cells by light and electron microscopy, 2-D separation of proteins, and confocal microscopy has confirmed the existence of an organized nucleolar matrix with a complex protein composition to which are attached the insoluble processing complexes. In the present work, we present evidence from immunoblotting, immunofluorescence, immunogold labeling, and preferential cytochemical staining with bismuth salts that an insoluble fraction of the multifunctional protein nucleolin, is a component of the onion nucleolar matrix, and analyse its ultrastructural distribution in the described domains of the matrix.
Laboratorio de Biología Celular y Molecular Vegetal, Centro de Investigaciones Biológicas, CSIC, Velázquez, Madrid, Spain.
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Minguez
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Moreno Diaz de la Espina
D000490:Allium / Q000737:chemistry* / Q000648:ultrastructure; D001729:Bismuth; D002466:Cell Nucleolus / Q000737:chemistry*; D004396:Coloring Agents; D008970:Molecular Weight; D015530:Nuclear Matrix / Q000737:chemistry* / Q000648:ultrastructure; D009687:Nuclear Proteins / Q000032:analysis* / Q000737:chemistry; D010750:Phosphoproteins / Q000032:analysis* / Q000737:chemistry; D010940:Plant Proteins / Q000032:analysis*; D016601:RNA-Binding Proteins; D000097796:Nucleolin
D001729:Bismuth; D004396:Coloring Agents; D009687:Nuclear Proteins; D010750:Phosphoproteins; D010940:Plant Proteins; D016601:RNA-Binding Proteins; D000097796:Nucleolin