[Relationship between amplicon composition and cytologic type of structures containing amplified DNA in murine P388 cells with multiple drug resistance].
Previously, we showed that, development of multidrug resistance (MDR) in mouse P388 leukemia cells, is often associated with the appearance of newly-formed chromosome-like structures that contain amplified copies of the mdrl gene. In the present study, we compared amplicon content in P388 sublines showing different types of these structures. A strong correlation between the formation of specific acentric markers consisting of two identical arms and the absence of the sorcin gene co-amplification was found. In all the sublines containing other types of chromosome-like structures, the sorcin gene is co-amplified.
Il'inskaia
G V
GV
Demidova
N S
NS
Kopnin
B P
BP
D000818:Animals; D002135:Calcium-Binding Proteins / Q000235:genetics*; D002875:Chromosomes; D018432:Drug Resistance, Multiple / Q000235:genetics*; D019008:Drug Resistance, Neoplasm / Q000235:genetics*; D005784:Gene Amplification; D005819:Genetic Markers; D051379:Mice; D009363:Neoplasm Proteins / Q000235:genetics*; D014407:Tumor Cells, Cultured
D002135:Calcium-Binding Proteins; D005819:Genetic Markers; D009363:Neoplasm Proteins; C503114:Sri protein, mouse
Sviaz' mezhdu sostavom amplikonov i tsitologicheskim tipom struktur, soderzhashchikh amplifitsirovannuiu DNK v myshinykh kletkakh P388 so mnozhestvennoĭ lekarstvennoĭ ustoĭchivost'iu.