Protective effective of vitamin E in kidney storage solution on renal tissue metabolism in rats.
OBJECTIVE
To determine whether inclusion of vitamin E into kidney storage solutions protects metabolism and tubular ultrastructure of stored rat kidney.


METHODS
Rat kidneys were flush stored in Marshall's Citrate (MC) and MC + vitamin E (25% of LD 50 and 50% of LD 50) for 24 hours at 0 degrees C. After storage kidney slices were tested for gluconeogenesis and lactate dehydrogenase (LDH) activity, and examined for cellular ultrastructure.


RESULTS
Kidneys stored in MC + vitamin E gave higher gluconeogenesis than those stored in MC alone (p < 0.001). Tubular ultrastructure was better preserved in the presence of Vitamin E.


CONCLUSIONS
Vitamin E appears to protect the metabolism and ultrastructure of stored rat kidneys.
Selliah
S
S
Department of Physiology, Faculty of Medicine, University of Kelaniya.
de Mel
T
T
Hewamadduma
C A
CA
de Tissera
A
A
D000818:Animals; D005943:Gluconeogenesis / Q000187:drug effects*; D007668:Kidney / Q000378:metabolism* / Q000648:ultrastructure; D009926:Organ Preservation; D051381:Rats; D017207:Rats, Sprague-Dawley; D014810:Vitamin E / Q000494:pharmacology*
D014810:Vitamin E