Analysis of the 5' end of the rat plasma membrane Ca(2+)-ATPase isoform 3 gene and identification of extensive trinucleotide repeat sequences in the 5' untranslated region.
We have characterized the 5' end of the rat gene encoding isoform 3 of the plasma membrane Ca(2+)-ATPase using S1 nuclease protection and DNA sequence analysis. The 5'-untranslated region consists of over 900 nucleotides and includes a 217-nucleotide sequence composed of alternating tracts of TCC and ACC trinucleotides. Analysis of genomic sequences 5' to the transcription initiation site revealed potential binding sites for transcription factors that are active in muscle and brain.
Department of Molecular Genetics, Biochemistry and Microbiology, University of Cincinnati College of Medicine, OH 45267-0524, USA.
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Burk
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Menon
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Shull
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D001921:Brain / Q000378:metabolism; D000252:Calcium-Transporting ATPases / Q000235:genetics*; D002462:Cell Membrane / Q000201:enzymology*; D018076:DNA, Complementary / Q000032:analysis; D008969:Molecular Sequence Data; D009132:Muscles / Q000378:metabolism; D051381:Rats; D017422:Sequence Analysis, DNA; D015719:Single-Strand Specific DNA and RNA Endonucleases; D018911:Trinucleotide Repeats
D018076:DNA, Complementary; D015719:Single-Strand Specific DNA and RNA Endonucleases; D000252:Calcium-Transporting ATPases