The novel monoclonal antibody By114 helps detect the early emergence of a paroxysmal nocturnal hemoglobinuria clone in aplastic anemia.
The newly described monoclonal antibody By114 has been used with flow cytometry to investigate the status of the 90-kD glycosylphosphatidyl-inositol (GPI)-anchored component of CD66 (CD66c) on neutrophils from nine patients with paroxysmal nocturnal hemoglobinuria (PNH), seven with aplastic anemia/PNH, and 63 with aplastic anemia (AA) and a negative Ham's test. We have found that By114 is a sensitive indicator for recognizing patients with PNH and has helped delineate a group of nine patients with aplastic anemia and a negative Ham's test who have evidence of a larger PNH clone than indicated by other monoclonal antibodies (mAbs). By114 is a valuable marker for detecting the emergence of a PNH clone before the Ham's test becomes positive and is a more sensitive detector of deficient GPI-anchored proteins than other mAbs.
Department of Cellular and Molecular Sciences, St George's Hospital Medical School, London, UK.
J A
JA
Tooze
R
R
Saso
J C
JC
Marsh
A
A
Papadopoulos
K
K
Pulford
E C
EC
Gordon-Smith
D000293:Adolescent; D000328:Adult; D000368:Aged; D000741:Anemia, Aplastic / Q000150:complications*; D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000097:blood; D000943:Antigens, Differentiation / Q000097:blood; D015815:Cell Adhesion Molecules; D002648:Child; D004912:Erythrocytes / Q000737:chemistry; D005260:Female; D005434:Flow Cytometry; D019084:Fluorescent Antibody Technique, Indirect; D017261:Glycosylphosphatidylinositols / Q000032:analysis* / Q000172:deficiency; D006457:Hemoglobinuria, Paroxysmal / Q000097:blood / Q000150:complications / Q000175:diagnosis*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000737:chemistry; D009504:Neutrophils / Q000737:chemistry
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000943:Antigens, Differentiation; C069847:CD66 antigens; D015815:Cell Adhesion Molecules; D017261:Glycosylphosphatidylinositols