Receptors for the V4 strain of Newcastle disease virus in the digestive tract of chickens.
Enterocytes were detached from various parts of the digestive tract of chickens by treatment with DTT or with hyaluronidase. Isolated enterocytes were exposed to suspensions of the V4 strain of Newcastle disease virus (NDV). Removal of virus from the supernatant fluid was taken as evidence of binding of virus to enterocytes and residual virus was measured both by infectivity assay and by ELISA. Enterocytes from duodenum, jejunum, ileum, caecum, and rectum bound the virus; enterocytes from oesophagus, crop and proventriculus did not.
Department of Veterinary Pathology, University of Queensland, Brisbane, Australia.
S F
SF
Rehmani
P B
PB
Spradbrow
D000818:Animals; D002469:Cell Separation; D002645:Chickens; D004064:Digestive System / Q000821:virology*; D004068:Digestive System Physiological Phenomena; D004797:Enzyme-Linked Immunosorbent Assay; D004848:Epithelium / Q000502:physiology / Q000821:virology; D006821:Hyaluronoglucosaminidase; D007413:Intestinal Mucosa / Q000502:physiology / Q000821:virology; D009522:Newcastle disease virus / Q000502:physiology*; D009928:Organ Specificity; D011991:Receptors, Virus / Q000502:physiology*
D011991:Receptors, Virus; D006821:Hyaluronoglucosaminidase