Biodistribution and dosimetry of indium-111-polyclonal IgG in normal subjects.
Indium-111-polyclonal IgG is a new imaging agent of infection and inflammation that has been developed as a possible replacement for radiolabeled leukocytes. We undertook a study to determine the safety, biodistribution and dosimetry of the agent in normal subjects.


METHODS
Twelve normal male volunteers with an average age of 34 yr (range 21-55 yr) were studied. Each was injected with 1.22-1.47 mCi 111In-labeled polyclonal IgG; digital whole-body images, in addition to blood, urine and fecal samples, were obtained immediately after injection and at 6, 24, 48, 72, 96 and 120 hr. Whole-body counts, as well as individual organ data obtained by outlining regions of interest, were measured. Blood, urine and fecal counting were done in a well counter and compared to known standards; dosimetry calculations were performed with the MIRD technique.


RESULTS
The mean whole-blood activity had a two-phase disappearance curve: the T1/2I was 11.4 hr (61.1%) and the T1/2II was 112.5 hr (38%). Twelve percent of the dose was excreted in the urine and 1.14% in the feces. Skeletal muscle had the highest percentage of uptake, followed by the bone marrow, liver and lungs; the spleen showed less than 1% uptake. Activity in the lungs varied with time, falling by 37% after 18 hr and by 68% after 72 hr. Dosimetry calculations indicated that the highest absorbed dose was to the liver (1.42 rad/mCi) followed by the testes (1.23 rad/mCi) and red marrow (0.976 rad/mCi). The total-body dose was 0.467 rad/mCi, with an effective dose equivalent of 790.84 mrem.


CONCLUSIONS
The biodistribution of 111In IgG is similar to that of 99mTc-HMPAO-labeled leukocytes. Activity in the liver, kidneys and GI tract may make evaluation of infection in these regions difficult. The dosimetry data indicate that adequate doses can be administered for clinical imaging without exposing the patient to excessive radiation.
Datz
F L
FL
Department of Radiology, University of Utah School of Medicine, Salt Lake City 84132, USA.
Castronovo
F P
FP
Christian
P E
PE
Anderson
C E
CE
Crebs
K
K
Morton
K A
KA
Rauh
D A
DA
D000328:Adult; D005240:Feasibility Studies; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism; D007205:Indium Radioisotopes / Q000493:pharmacokinetics; D008297:Male; D004369:Pentetic Acid / Q000031:analogs & derivatives* / Q000378:metabolism; D011829:Radiation Dosage; D016719:Radioimmunodetection; D014018:Tissue Distribution
C069971:DTPA-IgG complex; D007074:Immunoglobulin G; D007205:Indium Radioisotopes; D004369:Pentetic Acid