The function of the octamer-binding site in the DRA promoter.
The octamer binding site, which is located immediately upstream of the poorly conserved DRA TATA sequence, is important for high levels of expression of this human major histocompatibility class II gene in B cells. In this study, we demonstrate that the substitution of the DRA TATA sequence with the TATA box from the adenovirus E1b promoter removes the requirement for the octamer binding site for high levels of expression from the DRA promoter. Since only the TATA box from the E1b but not the DRA promoters binds the TATA binding protein, we conclude that the octamer binding site helps to recruit TBP to the DRA promoter.
Searle Research and Development, Monsanto Company, St. Louis, MO 63198, USA.
C F
CF
Voliva
N
N
Jabrane-Ferrat
B M
BM
Peterlin
D017501:Adenovirus E1B Proteins / Q000235:genetics*; D001483:Base Sequence; D001665:Binding Sites; D002051:Burkitt Lymphoma / Q000473:pathology; D004274:DNA, Recombinant / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism*; D006684:HLA-DR Antigens / Q000235:genetics*; D059805:HLA-DR alpha-Chains; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D050813:Octamer Transcription Factor-2; D011401:Promoter Regions, Genetic; D012045:Regulatory Sequences, Nucleic Acid; D016385:TATA Box; D035181:TATA-Box Binding Protein; D035362:Transcription Factor TFIID; D014157:Transcription Factors / Q000378:metabolism*; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
D017501:Adenovirus E1B Proteins; D004274:DNA, Recombinant; D004268:DNA-Binding Proteins; D006684:HLA-DR Antigens; D059805:HLA-DR alpha-Chains; D050813:Octamer Transcription Factor-2; C058435:POU2F2 protein, human; D035181:TATA-Box Binding Protein; D035362:Transcription Factor TFIID; D014157:Transcription Factors