A cDNA encoding a new member of the rat placental lactogen family, PL-I mosaic (PL-Im).
We have isolated a cDNA encoding a novel placental lactogen (PL), PL-I mosaic (PL-Im), by screening the cDNA library in lambda ZAP of the mid-pregnant (day 12) rat placenta. The cDNA comprised an open reading frame of 687 bp encoding 229 amino acids, in which there were two putative N-glycosylation sites. Northern blot analysis showed that PL-Im mRNA was expressed specifically during mid-pregnancy (days 10 and 12) in the rat placenta. The cDNA was highly homologous with those of other rat PL family members; in particular, the homology among PL-Im, PL-I (mid-pregnancy-specific) and PL-Iv (late-pregnancy-specific) was over 90%. Interestingly, the nucleotide sequence of PL-Im cDNA was a mosaic of PL-I and PL-Iv and it did not possess its own particular sequence. As the genomic pattern determined using Southern blot analysis of PL-Im was distinct from that of PL-I and PL-Iv, the encoded area of PL-Im appears to be independent of those of PL-I and PL-Iv on the gene. In the dendrogram of the rat PL family constructed on the basis of the nucleotide sequence homologies, PL-Im was located between PL-Iv and PL-I in the process of molecular evolution. Therefore, PL-Im has a unique cDNA structure and may be a principal factor in the molecular evolution of PLs.
Laboratory of Cellular Biochemistry, University of Tokyo, Japan.
M
M
Hirosawa
R
R
Miura
K S
KS
Min
N
N
Hattori
K
K
Shiota
T
T
Ogawa
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000032:analysis*; D005260:Female; D008297:Male; D008969:Molecular Sequence Data; D010920:Placenta / Q000378:metabolism*; D010928:Placental Lactogen / Q000235:genetics / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar
D018076:DNA, Complementary; D012333:RNA, Messenger; C085699:placental lactogen I, rat; D010928:Placental Lactogen