Corticotropin-releasing factor treatment upregulates interleukin-1 receptors in the mouse pituitary: reversal by dexamethasone.
Intraperitoneal injection of rat/human corticotropin-releasing factor (CRF) (40 micrograms/kg/0.2 ml of saline) resulted in a dramatic increase in specific iodine-125-labeled human interleukin-1 alpha ([125I]IL-1 alpha) binding in the male C57BL/6 mouse pituitary at 2 and 6 h after the injection although it did not affect [125I]IL-1 alpha binding in the mouse hippocampus, spleen and testis at any time after the injection. [125I]IL-1 alpha binding was unchanged at 2 h following dexamethasone (DEX) treatment (1 mg/kg/0.2 ml of 4% ethanol-saline) in the mouse pituitary and the hippocampus. In contrast, DEX inhibited CRF-induced upregulation of IL-1 receptors in the pituitary at 2 h after the injection. These data demonstrate complex interactions between CRF and DEX on IL-1 receptors during stress.
Second Department of Internal Medicine, Kochi Medical School, Japan.
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Takao
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Tojo
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Nishioka
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Hashimoto
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De Souza
D000324:Adrenocorticotropic Hormone / Q000097:blood; D000704:Analysis of Variance; D000818:Animals; D003345:Corticosterone / Q000097:blood; D003346:Corticotropin-Releasing Hormone / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D003907:Dexamethasone / Q000494:pharmacology*; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010902:Pituitary Gland / Q000187:drug effects* / Q000378:metabolism; D011869:Radioligand Assay; D051381:Rats; D017472:Receptors, Interleukin-1 / Q000187:drug effects*; D013312:Stress, Physiological / Q000378:metabolism*; D015854:Up-Regulation / Q000187:drug effects
D017472:Receptors, Interleukin-1; D003907:Dexamethasone; D000324:Adrenocorticotropic Hormone; D003346:Corticotropin-Releasing Hormone; D003345:Corticosterone