Assessment of liposomal transfection of ocular tissues in vivo.
A possible route to the treatment of inherited retinal degenerations such as retinitis pigmentosa (RP) is the application of somatic gene therapy by the transfer and expression of corrective functional genes in ocular tissue. Cationic liposomes are established vehicles for the delivery and expression of exogenous genes in mammalian cells both in vitro and in vivo1. We report here a preliminary assessment of liposome-mediated transfer of a plasmid carrying the reporter gene lacZ (encoding the enzyme beta-galactosidase) into tissues of the adult rabbit eye.
Retinitis Pigmentosa Laboratory, Rayne Institute, St Thomas's Hospital, London, U.K.
S E
SE
Jones
J D
JD
McHugh
C
C
Jomary
A
A
Shallal
M J
MJ
Neal
D000818:Animals; D004274:DNA, Recombinant; D005123:Eye / Q000201:enzymology; D008081:Liposomes; D008863:Microspheres; D011401:Promoter Regions, Genetic; D011817:Rabbits; D012174:Retinitis Pigmentosa / Q000628:therapy; D013539:Simian virus 40 / Q000235:genetics; D014162:Transfection / Q000379:methods*; D001616:beta-Galactosidase / Q000235:genetics* / Q000378:metabolism
D004274:DNA, Recombinant; D008081:Liposomes; D001616:beta-Galactosidase