Analysis of the replication direction through the domain of alpha-globin-encoding chicken genes.
The polarity of leading DNA strand synthesis through the domain of alpha-globin-encoding genes in chicken erythroid cells has been studied using a modification of the previously published replication direction assay [Handeli et al., Cell 57 (1989) 909-920; Burhans et al., EMBO J. 10 (1991) 4351-4360]. In accordance with our previous observations [Razin et al., Nucleic Acids Res. 14 (1986) 8189-8207], it has been found that a replication origin (ori) is located in the area extending from 8.5 to 2.5 kb upstream from the pi gene, the first gene of the domain. The whole domain is replicated from this ori in the direction of transcription. The replication termination site has been mapped right after the last gene of the domain (alpha A gene) at a distance of about 12 kb from the ori.
Verbovaia
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International Centre for Genetic Engineering and Biotechnology, Trieste, Italy.
Razin
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D000818:Animals; D001483:Base Sequence; D002645:Chickens / Q000235:genetics; D004261:DNA Replication; D005796:Genes; D005914:Globins / Q000235:genetics*; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes / Q000737:chemistry
D015345:Oligonucleotide Probes; D005914:Globins