Systemic antibody responses of calves to low molecular weight Cooperia oncophora antigens.
The systemic antibody responses to adult Cooperia oncophora antigen were studied using sera obtained from calves during a 6-week period following a single oral infection with either 20,000 or 100,000 third-stage C. oncophora larvae. Dose dependent increasing titres of IgG binding complete adult Cooperia antigen were found in the sera of Cooperia-infected calves. SDS-gel electrophoresis under reducing conditions, followed by Western blotting, revealed that the increase of IgG binding Cooperia antigens could be attributed mainly to specific binding of IgG to a complex of 12-15 kDa protein fragments of Cooperia adult antigen. This protein may represent a Cooperia oncophora-specific component that can be used for serodiagnosis.
Nieuwland
M G
MG
Department of Animal Husbandry, Agricultural University, Wageningen, Netherlands.
Ploeger
H W
HW
Kloosterman
A
A
Parmentier
H K
HK
D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis / Q000097:blood*; D000917:Antibody Formation; D000937:Antigen-Antibody Reactions; D000947:Antigens, Helminth / Q000276:immunology* / Q000302:isolation & purification; D015153:Blotting, Western; D002417:Cattle; D002418:Cattle Diseases; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000097:blood; D007814:Larva; D008297:Male; D009348:Nematoda / Q000276:immunology*; D009349:Nematode Infections / Q000097:blood / Q000276:immunology / Q000662:veterinary*; D010270:Parasite Egg Count; D013997:Time Factors
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D007074:Immunoglobulin G