The development and validation of a high performance liquid chromatography (HPLC)/tandem mass spectrometry assay for fenticonazole in human plasma and comparison with an HPLC-UV method.
A method is described for the determination of fenticonazole in human female plasma. The method utilizes high performance liquid chromatography coupled to atmospheric pressure positive-ion chemical ionization triple quadrupole mass spectrometry. Multiple reaction monitoring is employed for selectivity and sensitivity which enables quantification over the range 0.5-20 ng mL-1 with acceptable precision and accuracy. A comparison is made with an existing HPLC-UV assay and the utility of the technology of combined liquid chromatography and tandem mass spectometry for subnanogram per mL assays is discussed.
Upjohn Laboratories--Europe, West Sussex, UK.
W
W
Speed
J M
JM
Long
R J
RJ
Simmonds
T A
TA
Enos
D000935:Antifungal Agents / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D005260:Female; D005615:Freezing; D006801:Humans; D007093:Imidazoles / Q000097:blood*; D007202:Indicators and Reagents; D013058:Mass Spectrometry; D013056:Spectrophotometry, Ultraviolet
D000935:Antifungal Agents; D007093:Imidazoles; D007202:Indicators and Reagents; C033486:fenticonazole