Sequencing and linkage analysis of a Coxiella burnetii 2.1 kb NotI fragment.
Most of the Coxiella burnetii isolates (72 of 80) available in our institute share a 2.1 kb NotI fragment. Sequence analysis revealed two incomplete open reading frames (ORF) for putative genes hemA and bcR coding for glutamyl-tRNA-reductase and bicyclomycin resistance protein, respectively. Upon completing the ORF for the hemA gene by sequencing the adjacent NotI/EcoRI fragment a third ORF for the putative prfA gene was determined coding for release factor 1, an enzyme catalysing the last step in protein biosynthesis.
Institut für Hygiene und Infektionskrankheiten der Tiere, Justus-Liebig-Universität, Giessen, Germany.
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D000445:Aldehyde Oxidoreductases / Q000235:genetics; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000494:pharmacology; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D016997:Coxiella burnetii / Q000235:genetics*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000235:genetics*; D004352:Drug Resistance, Microbial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D029968:Escherichia coli Proteins; D005798:Genes, Bacterial / Q000235:genetics; D008040:Genetic Linkage; D008969:Molecular Sequence Data; D015513:Oncogene Proteins / Q000235:genetics; D016366:Open Reading Frames / Q000235:genetics; D010454:Peptide Termination Factors / Q000235:genetics; D011505:Protein-Tyrosine Kinases; D011518:Proto-Oncogene Proteins; D051562:Proto-Oncogene Proteins c-bcr; D012486:Salmonella typhimurium / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific) / Q000235:genetics
D000900:Anti-Bacterial Agents; D019086:Bridged Bicyclo Compounds, Heterocyclic; D029968:Escherichia coli Proteins; D015513:Oncogene Proteins; D010454:Peptide Termination Factors; D011518:Proto-Oncogene Proteins; C489005:prfA protein, E coli; D000445:Aldehyde Oxidoreductases; C067282:glutamyl tRNA reductase; C020098:DNA modification methylase EcoRI; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific); D011505:Protein-Tyrosine Kinases; D051562:Proto-Oncogene Proteins c-bcr; D015252:Deoxyribonucleases, Type II Site-Specific; C038565:GCGGCCGC-specific type II deoxyribonucleases; C024504:bicozamycin