Interaction of cryptophycin 1 with tubulin and microtubules.
The cryptophycins are newly discovered antimitotic agents isolated from the cyanobacterium Nostoc. Previous studies using cultured cells demonstrated that microtubules are the target of these compounds. We have studied the interaction of cryptophycin 1 with tubulin and microtubules in vitro. Cryptophycin 1 is an effective inhibitor of tubulin polymerization, causes tubulin to aggregate, and depolymerizes microtubules to linear polymers somewhat similar to the spiral-like structures produced by the Vinca alkaloids. Cryptophycin 1 also inhibits vinblastine binding to tubulin but not colchicine binding. Thus, it appears that the cryptophycins may bind to the Vinca site in tubulin or to a site that overlaps with the Vinca site.
Department of Biochemistry, University of Kansas, Lawrence 66045, USA.
K
K
Kerksiek
M R
MR
Mejillano
R E
RE
Schwartz
G I
GI
Georg
R H
RH
Himes
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001665:Binding Sites; D001923:Brain Chemistry; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D003078:Colchicine / Q000378:metabolism; D047630:Depsipeptides; D046911:Macromolecular Substances; D008854:Microscopy, Electron; D008870:Microtubules / Q000187:drug effects* / Q000648:ultrastructure; D010456:Peptides, Cyclic / Q000378:metabolism / Q000494:pharmacology*; D014404:Tubulin / Q000737:chemistry / Q000378:metabolism; D050257:Tubulin Modulators; D014747:Vinblastine / Q000378:metabolism
D000970:Antineoplastic Agents; D047630:Depsipeptides; D046911:Macromolecular Substances; D010456:Peptides, Cyclic; D014404:Tubulin; D050257:Tubulin Modulators; C088419:cryptophycin; D014747:Vinblastine; D003078:Colchicine