Anion-exchange HPLC analysis of biotinylated oligonucleotides.
Biotinylated oligonucleotides combined with streptavidin-coated magnetic beads are commonly used in current molecular biology. Their quality and the level of incorporated biotin are essential for yielding good results in either solid-phase DNA sequencing or solid-phase purification procedures. This paper presents a very simple analytical test using anion-exchange HPLC and avidin to ascertain the quality of biotinylated oligonucleotides and to predetermine their ability to bind to avidin, which is a prerequisite for functionality in some solid-phase methods.
Istituto di Tecnologie, Biomediche Avanzate, Segrate, Italy.
G
G
De Bellis
L
L
Invernizzi
S
S
Debernardi
R
R
Pergolizzi
M
M
Luzzana
D001360:Avidin / Q000737:chemistry; D001483:Base Sequence; D001710:Biotin / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002852:Chromatography, Ion Exchange; D017931:DNA Primers; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000032:analysis*
D017931:DNA Primers; D009841:Oligonucleotides; D001360:Avidin; D001710:Biotin