[Detection of Mycobacterium tuberculosis complex with polymerase chain reaction (PCR)].
248 respiratory specimens were examined using by Nested-PCR and by conventional methods (solid and liquid media). 54 specimens were culture-positive for M. tuberculosis, 51 of these were detected by PCR and conventional methods, whereas 3 were missed by PCR and 15 were only positive by PCR. 12 of the 15 culture-negative specimens derived from patients under treatment for tuberculosis. The 3 specimens, which are PCR negative could also be detected after modification of the method. The very high sensitivity depends on the high positive rate (21.8%) of the investigated specimens, so it is necessary to look also for other than pulmonary specimens, but it seems that his Nested-PCR is a highly specific and sensitive method for detecting of M. tuberculosis.
Forschungsinstitut Borstel.
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Schröder
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Schindler
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Rüsch-Gerdes
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Kövary
D001992:Bronchoalveolar Lavage Fluid / Q000382:microbiology; D006801:Humans; D009165:Mycobacterium Infections, Nontuberculous / Q000175:diagnosis* / Q000382:microbiology; D015269:Mycobacterium avium Complex / Q000302:isolation & purification; D015270:Mycobacterium avium-intracellulare Infection / Q000175:diagnosis* / Q000382:microbiology; D009163:Mycobacterium bovis / Q000302:isolation & purification*; D009169:Mycobacterium tuberculosis / Q000302:isolation & purification*; D009170:Nontuberculous Mycobacteria / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013183:Sputum / Q000382:microbiology; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis* / Q000382:microbiology
Nachweis von Mycobacterium tuberculosis-Komplex mit der Polymerase-Kettenreaktion (PCR).