Spectroscopic studies of galactose oxidase.
A combination of powerful spectroscopic approaches is now available for detailed characterization of active sites in free radical metalloenzymes. As illustrated for galactose oxidase, a single approach such as EPR or absorption spectroscopy is not sufficient to characterize a sample completely, and only by combining results from complementary techniques can the information in each approach be used most effectively.
Department of Chemistry, Carnegie-Mellon University, Pittsburgh, Pennsylvania 15213, USA.
J W
JW
Whittaker
D000595:Amino Acid Sequence; D001665:Binding Sites; D002942:Circular Dichroism; D003300:Copper / Q000032:analysis; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods; D005692:Galactose Oxidase / Q000737:chemistry* / Q000378:metabolism; D008024:Ligands; D008280:Magnetics; D011048:Polarography; D011487:Protein Conformation; D013053:Spectrophotometry / Q000379:methods
D008024:Ligands; D003300:Copper; D005692:Galactose Oxidase