Cloning and analysis of the coding region of the histone H1(0)-encoding gene from rat PC12 cells.
We have determined the complete coding sequence of the histone-encoding H1(0) gene from rat PC12 cells. Southern and Northern analyses suggest that rat H1(0) is encoded by a single-copy gene which generates an mRNA of about 2.2 kb. Comparison of the rat, mouse and human amino-acid sequences shows that the C-terminal domain of the protein is much more variable than the N-terminal and central domains. Rates of non-synonymous and synonymous nucleotide substitution have been calculated. The rate of non-synonymous substitution is about 2.5-times higher in the rodent lineage than in the human lineage.
Martínez
P
P
Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad Autonóma de Barcelona, Bellaterra, Spain.
Vidal
J M
JM
Monsalves
C
C
Pérez
M
M
Pucket
C
C
Ponte
I
I
Suau
P
P
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005075:Biological Evolution; D003001:Cloning, Molecular; D015870:Gene Expression; D005796:Genes; D006657:Histones / Q000235:genetics*; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D016716:PC12 Cells; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
D006657:Histones; D012333:RNA, Messenger