Listeriolysin is a potent inducer of the phosphatidylinositol response and lipid mediator generation in human endothelial cells.
The impact of Listeria monocytogenes listeriolysin O (LLO) secretion on phosphoinositide metabolism and mediator (platelet-activating factor and prostaglandin I2) generation was investigated in human umbilical vein endothelial cells. Wild-type L. monocytogenes, purified LLO, and an L. innocua strain engineered to secrete LLO all elicited a strong response, whereas mutant strains defective in LLO production were ineffective. Thus, human umbilical vein endothelial cell stimulation by listeriae is linked to production of LLO.
Department of Internal Medicine, Justus Liebig University, Giessen, Federal Republic of Germany.
U
U
Sibelius
F
F
Rose
T
T
Chakraborty
A
A
Darji
J
J
Wehland
S
S
Weiss
W
W
Seeger
F
F
Grimminger
D001427:Bacterial Toxins; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000378:metabolism; D011464:Epoprostenol / Q000096:biosynthesis*; D006360:Heat-Shock Proteins / Q000494:pharmacology*; D006460:Hemolysin Proteins / Q000494:pharmacology*; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D010716:Phosphatidylinositols / Q000096:biosynthesis*; D010972:Platelet Activating Factor / Q000096:biosynthesis*
D001427:Bacterial Toxins; D006360:Heat-Shock Proteins; D006460:Hemolysin Proteins; D010716:Phosphatidylinositols; D010972:Platelet Activating Factor; D011464:Epoprostenol; D007770:L-Lactate Dehydrogenase; C038705:hlyA protein, Listeria monocytogenes