Bioactivity of recombinant feline interleukin-2 on human and feline leukocytes.
Interleukin (IL)-2 is a 16,000 Da protein product of T lymphocytes which is the principle cytokine responsible for clonal expansion of T lymphocytes as a response to antigen exposure. Deficiency of functional IL-2 plays a pivotal role in the pathogenesis of human immunodeficiency syndrome and may be important in the pathogenesis of feline immunodeficiency syndrome as well. Additionally, IL-2 may enhance secretion of interleukin-5 from the TH2 subset of CD4+ T cells, promote peripheral and systemic eosinophilia, and contribute to the eosinophilia which characterizes the inflamed airways of human beings and cats with asthma. We recently reported the sequence of feline IL-2 and the synthesis of recombinant feline IL-2. The purpose of the present study was to evaluate the bioactivity of recombinant feline IL-2 on human and feline leukocytes. We established dose-response relationships between recombinant feline IL-2 and radiolabeled proliferating human and feline leukocytes using thymidine incorporation as a marker of bioactivity. We found that recombinant human IL-2 promotes proliferation of both human and feline leukocytes. However, recombinant feline IL-2 promotes proliferation of feline cells, but not human cells.
Cozzi
P J
PJ
Department of Medicine, University of Chicago, IL 60637, USA.
Padrid
P
P
Tompkins
M B
MB
Alegre
M L
ML
Takeda
J
J
Leff
A R
AR
D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D002415:Cats; D002455:Cell Division / Q000187:drug effects; D003602:Cytotoxicity, Immunologic / Q000187:drug effects; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D006801:Humans; D007376:Interleukin-2 / Q000276:immunology / Q000494:pharmacology*; D007962:Leukocytes / Q000187:drug effects / Q000276:immunology*; D008213:Lymphocyte Activation / Q000187:drug effects; D008297:Male; D011994:Recombinant Proteins / Q000494:pharmacology*; D013602:T-Lymphocytes, Cytotoxic / Q000187:drug effects / Q000276:immunology
D000906:Antibodies; D007376:Interleukin-2; D011994:Recombinant Proteins