A new family of lipolytic plant enzymes with members in rice, arabidopsis and maize.
We have noted a striking similarity between the sequences of proteins in a novel family of lipases we recently reported [Upton, C. and Buckley, J. T. (1995) Trends Biol. Sci. 20, 178-9] and more than 120 sequences from the database of Expressed Sequence Tags (dbEST) which correspond to at least 30 unique genes from arabidopsis, rice and maize. A cDNA (Arab-1) corresponding to one of these sequences was isolated, sequenced and translated. There was significant similarity to sequences in the new lipase family over the entire open reading frame of Arab-1 and when expressed in E. coli, the gene product was lipolytic. Arab-1 and genes for some of the other plant proteins appear to be differentially expressed. They may play a role in the regulation of lipid metabolism during plant development.
Brick
D J
DJ
Department of Biochemistry and Microbiology, University of Victoria, B.C. Canada.
Brumlik
M J
MJ
Buckley
J T
JT
Cao
J X
JX
Davies
P C
PC
Misra
S
S
Tranbarger
T J
TJ
Upton
C
C
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics; D001483:Base Sequence; D002087:Butyrates / Q000378:metabolism; D018076:DNA, Complementary / Q000235:genetics; D015870:Gene Expression; D008049:Lipase / Q000235:genetics*; D008066:Lipolysis / Q000235:genetics*; D008969:Molecular Sequence Data; D012275:Oryza / Q000201:enzymology / Q000235:genetics; D010944:Plants / Q000201:enzymology* / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution; D014158:Transcription, Genetic; D003313:Zea mays / Q000201:enzymology / Q000235:genetics
D002087:Butyrates; D018076:DNA, Complementary; D012333:RNA, Messenger; C033592:4-nitrophenyl butyrate; D008049:Lipase