[Studies of liver macrophage function in the intraabdominal infection after partial hepatectomy in the rat model].
The activities of superoxide (O2-), tumor necrosis factor (TNF) and interleukin-1 (IL-1) of rat liver macrophage (M phi) were studied in the condition of intraabdominal infection (E group), hepatectomy (H group) and intraabdominal infection following hepatectomy (HE group). In the early stage of E and HE groups, O2- production was significantly increased after infection. In the H and HE groups, O2- production was significantly increased at 48 hours after hepatectomy, probably due to liver regeneration. In every group, the changes of TNF activity showed the similar pattern to those of O2- production. In H and E groups, the elevation of IL-1 activities was significantly increased. However in HE group, the IL-1 level remained low during 72 hours. When the M phi 48 hours following hepatectomy was stimulated by lipopolysaccharide (LPS), activities of TNF and IL-1 were correlated and reversely correlated with the LPS level, respectively. In the excessive stimulation by infection following hepatectomy, IL-1 activity is significantly suppressed with production of O2- and TNF by M phi maintained.
Okita
M
M
First Department of Surgery, Hiroshima University School of Medicine, Japan.
D000818:Animals; D004731:Endotoxins / Q000097:blood; D004927:Escherichia coli Infections / Q000276:immunology*; D006498:Hepatectomy / Q000379:methods; D007375:Interleukin-1 / Q000378:metabolism; D008099:Liver / Q000166:cytology*; D008115:Liver Regeneration; D008264:Macrophages / Q000276:immunology*; D008297:Male; D008940:Mitotic Index; D051381:Rats; D017208:Rats, Wistar; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D004731:Endotoxins; D007375:Interleukin-1; D014409:Tumor Necrosis Factor-alpha