Influence of 17 beta-estradiol and progesterone on rat ocular lens.
The effect of 17 beta-estradiol and progesterone on ocular lens in rats and untreated controls was studied. In the treated lenses, the activity of hexokinase and glucose-6-phosphate dehydrogenase remained unchanged. The activity of aldolase was increased in 18- and 20-month-old lenses as compared to controls. Aldose reductase activity was decreased at the age of 20 months (p < 0.001). Structural lens proteins studies by SDS polyacrylamide gel electrophoresis and immunodecoration with specific antibodies for crystallines alpha A + alpha B and beta + gamma suggest some protective effect in treated animals.
Department of Ophthalmology, Rambam Medical Center, Haifa, Israel.
J
J
Scharf
N
N
Azzam
D
D
Schapira
A
A
Dovrat
D
D
Gershon
M
M
Silbermann
D000449:Aldehyde Reductase / Q000378:metabolism; D000818:Animals; D003459:Crystallins / Q000378:metabolism; D003692:Delayed-Action Preparations; D004591:Electrophoresis, Polyacrylamide Gel; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D005634:Fructose-Bisphosphate Aldolase / Q000378:metabolism; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D006593:Hexokinase / Q000378:metabolism; D007908:Lens, Crystalline / Q000737:chemistry / Q000187:drug effects* / Q000201:enzymology; D011374:Progesterone / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
D003459:Crystallins; D003692:Delayed-Action Preparations; D011374:Progesterone; D004958:Estradiol; D000449:Aldehyde Reductase; D005954:Glucosephosphate Dehydrogenase; D006593:Hexokinase; D005634:Fructose-Bisphosphate Aldolase