Mitotic and meiotic detection of radiation-induced translocations in mouse stem cell spermatogonia using fluorescence in situ hybridization.
The efficiency of two methods of detection of translocations induced in mouse stem cell spermatogonia by X-ray doses of 2, 5 and 7 Gy was compared: classical multivalent analysis at diakinesis-metaphase I of meiosis and observation via fluorescence in situ hybridization analysis of mitotic or meiotic stages. Specific DNA libraries for chromosomes 1, 11 and 13 were used. The results obtained indicate that (a) chromosomes 1, 11 and 13 are more involved in multivalent formation than expected on the basis of DNA content and (b) if the mitotic FISH analysis data are corrected for the observed over-representation, the frequencies of induced translocations are similar to those recorded in the classical multivalent studies, suggesting equal scoring efficiencies in both systems.
van Buul
P P
PP
MGC-Department of Radiation Genetics and Chemical Mutagenesis, Leiden University, Sylvius Laboratory, The Netherlands.
Zandman
I M
IM
Grigorova
M
M
Boei
J J
JJ
Natarajan
A T
AT
D000818:Animals; D002871:Chromosome Banding; D002875:Chromosomes / Q000528:radiation effects*; D002999:Clone Cells / Q000528:radiation effects; D015342:DNA Probes; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008540:Meiosis; D008677:Metaphase; D051379:Mice; D008938:Mitosis; D013093:Spermatogonia / Q000166:cytology* / Q000528:radiation effects; D013234:Stem Cells / Q000528:radiation effects*; D014178:Translocation, Genetic
D015342:DNA Probes