Superoxide dismutase in SAS human tongue carcinoma cell line is a factor defining invasiveness and cell motility.
This article describes an apparent inverse relationship between cell motility and intracellular Cu-Zn superoxide dismutase (SOD) activity of two human squamous carcinoma-derived clones, SAS-H1 with high invasiveness and SAS-L1 with low invasiveness. Clone SAS-H1 exhibited significantly greater motility than SAS-L1 but had significantly lower levels of intracellular Cu-ZnSOD than SAS-L1 cells. We then transfected Cu-ZnSOD antisense cDNA into SAS-L1 to reduce the intracellular Cu-ZnSOD activity. Antisense cDNA transfected SAS-L-AS clones had lower Cu-ZnSOD activity than control vector-transfected SAS-L-Neo clones, and this was associated with increased motility. Invasiveness of SAS-H1 and SAS-L-AS1 was enhanced by superoxide treatment, while the invasiveness of SAS-L1 was unaffected. These findings indicate that intracellular SOD is involved in cell motility by virtue of its action in scavenging superoxide in the cells.
Muramatsu
H
H
Fourth Department of Internal Medicine, Sapporo Medical University School of Medicine, Hokkaido, Japan.
Kogawa
K
K
Tanaka
M
M
Okumura
K
K
Nishihori
Y
Y
Koike
K
K
Kuga
T
T
Niitsu
Y
Y
D001483:Base Sequence; D002294:Carcinoma, Squamous Cell / Q000201:enzymology* / Q000473:pathology*; D002465:Cell Movement; D017931:DNA Primers / Q000737:chemistry; D016373:DNA, Antisense; D006801:Humans; D008969:Molecular Sequence Data; D009361:Neoplasm Invasiveness; D013482:Superoxide Dismutase / Q000378:metabolism*; D013481:Superoxides / Q000378:metabolism / Q000494:pharmacology; D014062:Tongue Neoplasms / Q000201:enzymology* / Q000473:pathology*; D014407:Tumor Cells, Cultured
D017931:DNA Primers; D016373:DNA, Antisense; D013481:Superoxides; D013482:Superoxide Dismutase