Changes in proto-oncogene activity in the testis of the frog, Rana esculenta, during the annual reproductive cycle.
Proto-oncogenes are said to influence the regulation of cellular growth and differentiation. Myc, Fos, Jun, and Mos protein localization has been studied by immunocytochemistry in the testis of the frog, Rana esculenta, during the annual reproductive cycle. Oncoproteins have been localized in the primary and secondary (I and II) spermatogonia (SPG). Myc and Mos also appear in I and II spermatocytes (SPC) while Jun appears in II SPC. Myc, Fos, and Jun in SPG translocate in the nucleus during the periods of active spermatogenesis. Myc, Fos, and Jun are also localized in Sertoli cells. Fos is present in interstitial cells during the period characterized by the androgen peak which precedes the sharp increase of estradiol. It is suggested that proto-oncogene activity exerts a regulatory role in steroidogenesis and spermatogenesis.
Dipartimento di Fisiologia Umana e Funzioni Biologiche Integrate F. Bottazzi, II Università di Napoli, Italy.
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Chieffi
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Minucci
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Cobellis
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Fasano
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Pierantoni
D000728:Androgens / Q000378:metabolism; D000818:Animals; D004958:Estradiol / Q000378:metabolism; D016762:Genes, fos / Q000502:physiology; D016758:Genes, jun / Q000502:physiology; D016258:Genes, mos / Q000502:physiology; D016259:Genes, myc / Q000502:physiology; D007150:Immunohistochemistry; D008297:Male; D016760:Proto-Oncogene Proteins c-fos / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D016755:Proto-Oncogene Proteins c-jun / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D017231:Proto-Oncogene Proteins c-mos / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D016271:Proto-Oncogene Proteins c-myc / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D011519:Proto-Oncogenes / Q000502:physiology*; D011893:Rana esculenta / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D012098:Reproduction / Q000502:physiology*; D012621:Seasons; D012708:Sertoli Cells / Q000737:chemistry / Q000166:cytology / Q000378:metabolism; D013091:Spermatogenesis / Q000502:physiology; D013094:Spermatozoa / Q000737:chemistry / Q000166:cytology / Q000378:metabolism; D013737:Testis / Q000737:chemistry / Q000166:cytology / Q000502:physiology*
D000728:Androgens; D016760:Proto-Oncogene Proteins c-fos; D016755:Proto-Oncogene Proteins c-jun; D016271:Proto-Oncogene Proteins c-myc; D004958:Estradiol; D017231:Proto-Oncogene Proteins c-mos