Determination of vitamins A, E and K1 in milk by high-performance liquid chromatography with dual amperometric detection.
A high-performance liquid chromatographic method with dual-electrochemical detection (HPLC-DEC) for the simultaneous determination of vitamins A, E and K1 is described. Separation was carried out using a C18 reversed-phase column and 2.5 mmol l-1 acetic acid-sodium acetate in methanol-water (99 + 1, v/v) as the mobile phase. The compounds eluted with good resolution in the above mentioned order within about 20 min and were quantified by dual-amperometric detection with a glassy carbon electrode at -1100 mV (E1) and + 700 mV (E2) (versus Ag/AgCl). This reductive-oxidative detection mode gave reproducible results and the detection limits were of the order of 0.06, 0.19 and 3.1 ng for vitamins A, E and K1, respectively. The HPLC-DEC method was successfully applied to the analysis of vitamins A, E and K1 in liquid cows' milk and infant-formula powdered-milk samples after applying alkaline hydrolysis of the fatty material and extraction of the vitamins with hexane for the analysis of vitamins A and E. In the case of vitamin K1 it was necessary to carry out enzymic hydrolysis, since this vitamin is degraded in basic medium, followed by cleaning with a Sep-Pak silica cartridge to isolate it from the other vitamins. Good recoveries (between 81 and 106%) were obtained.
Delgado Zamarreño
M M
MM
Departmento de Química Analítica, Facultad de Ciencias Químicas, Universidad de Salamanca, Spain.
Sanchez Perez
A
A
Gomez Perez
M C
MC
Fernandez Moro
M A
MA
Hernandez Mendez
J
J
D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004563:Electrochemistry; D006801:Humans; D007223:Infant; D007225:Infant Food / Q000032:analysis; D008892:Milk / Q000737:chemistry*; D014815:Vitamins / Q000032:analysis*
D014815:Vitamins