The entire rne gene: the remaining questions.
A 30-fold overexpression of the entire rne gene results in a 3-fold increase in RNase E activity. The overexpression of cloned rne fragments suggests that synthesis of the Rne protein is autoregulated and this regulation is related to the RNase E activity of the gene product. The enzyme produced by the cloned gene has an enhanced thermolability. The thermal stability of the temperature-sensitive enzyme increases when the cells are cultivated in the presence of chloramphenicol, which suggests posttranslational alterations.
Department of Microbiology, Albert Szent-Györgyi University Medical School, Szeged, Hungary.
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Miczák
D000818:Animals; D002701:Chloramphenicol / Q000494:pharmacology; D004722:Endoribonucleases / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D051379:Mice
D002701:Chloramphenicol; D004722:Endoribonucleases; C022537:ribonuclease E