A YAC contig encompassing the recessive Stargardt disease gene (STGD) on chromosome 1p.
Stargardt disease (STGD) and fundus flavimaculatus are infrequent autosomal recessive conditions characterized by a juvenile macular dystrophy and variable degrees of peripheral retinal changes. Linkage analysis performed in 47 STGD/fundus flavimaculatus families demonstrated significant linkage to 13 polymorphic DNA markers on chromosome 1p. The maximum combined two-point lod score was 32.7 (maximum recombination fraction [phi max] = .006) with the polymorphic marker D1S188. Our data demonstrate that STGD and fundus flavimaculatus are the same disorder clinically and genetically and provide further evidence for genetic homogeneity of this phenotype. Analysis of recombination events on disease chromosomes placed the STGD gene within a 4-cM interval between markers D1S435 and D1S236. A physical map was constructed of a YAC contig flanking STGD, from markers D1S500 to D1S495, and includes the critical interval delineated by historical recombinants. This contig spans approximately 31 cM, with one gap (3-5 cM) that is outside the 4-cM critical region. Localization of STGD to a single YAC contig will facilitate its positional cloning.
Anderson
K L
KL
Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030-3498, USA.
Baird
L
L
Lewis
R A
RA
Chinault
A C
AC
Otterud
B
B
Leppert
M
M
Lupski
J R
JR
D000293:Adolescent; D001483:Base Sequence; D002648:Child; D002874:Chromosome Mapping; D018244:Chromosomes, Artificial, Yeast; D002878:Chromosomes, Human, Pair 1 / Q000235:genetics*; D005260:Female; D008040:Genetic Linkage; D006801:Humans; D008268:Macular Degeneration / Q000235:genetics*; D008297:Male; D008969:Molecular Sequence Data; D010375:Pedigree