Serine protease EpiP from Staphylococcus epidermidis catalyzes the processing of the epidermin precursor peptide.
The function of serine protease EpiP in epidermin biosynthesis was investigated. Epidermin is synthesized as a 52-amino-acid precursor peptide, EpiA, which is posttranslationally modified and processed to the mature 22-amino-acid peptide antibiotic. epiP was expressed in Staphylococcus carnosus with xylose-regulated expression vector pCX15. The cleavage of the unmodified EpiA precursor peptide to leader peptide and proepidermin by EpiP-containing culture filtrates of S. carnosus (pCX15epiP) was followed by reversed-phase chromatography and subsequent electrospray mass spectrometry.
Universität Tübingen, Federal Republic of Germany.
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S
Geissler
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F
Götz
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T
Kupke
D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D001430:Bacteriocins; D001483:Base Sequence; D004790:Enzyme Induction / Q000187:drug effects; D008969:Molecular Sequence Data; D010455:Peptides; D011498:Protein Precursors / Q000378:metabolism*; D011499:Protein Processing, Post-Translational / Q000502:physiology*; D021382:Protein Sorting Signals; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D017421:Sequence Analysis; D012697:Serine Endopeptidases / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D013210:Staphylococcus / Q000235:genetics; D013212:Staphylococcus epidermidis / Q000201:enzymology*; D014994:Xylose / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D001430:Bacteriocins; D010455:Peptides; D011498:Protein Precursors; D021382:Protein Sorting Signals; D011993:Recombinant Fusion Proteins; C050558:epidermin; D014994:Xylose; C097623:EpiP serine protease; D012697:Serine Endopeptidases