7-O-acetyl-GD3 in human T-lymphocytes is detected by a specific T-cell-activating monoclonal antibody.
The monoclonal antibody U5, which is a potent inducer of proliferation in human T-cells, was found to bind to an alkali-sensitive derivative of ganglioside GD3. Using immunochemical and spectroscopic methods, the structure of the U5 antigen was determined as 7-O-acetyl-GD3. The antibody U5 did not react with 9-O-acetyl-GD3 and bound severalfold more stronger to 7-O-acetyl-GD3 than to GD3. U5 is the first antibody known to detect preferentially 7-O-acetyl-GD3. Flow cytometric analysis showed that each major class of human leukocytes contained a significant fraction of cells binding the U5 antibody.
GBF-Gesellschaft für Biotechnologische Forschung mbH, Braunschweig, Federal Republic of Germany.
B
B
Kniep
C
C
Claus
J
J
Peter-Katalinic
D A
DA
Monner
W
W
Dippold
M
M
Nimtz
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D000918:Antibody Specificity; D002240:Carbohydrate Sequence; D002417:Cattle; D005260:Female; D005732:Gangliosides / Q000032:analysis* / Q000737:chemistry / Q000276:immunology*; D006801:Humans; D015151:Immunoblotting; D007700:Kinetics; D007962:Leukocytes / Q000276:immunology; D008213:Lymphocyte Activation / Q000187:drug effects; D013058:Mass Spectrometry; D008892:Milk / Q000737:chemistry; D008969:Molecular Sequence Data; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology*
C097213:7-O-acetyl-GD3 ganglioside; D000911:Antibodies, Monoclonal; D005732:Gangliosides; C026226:ganglioside, GD3