A simple dual pressure-ejection system and calibration method for brief local applications of drugs and modified salines.
We report a minimal method for dual pressure ejection through a single micropipette, which uses standard theta-type glass tubing and a connection through thinned polythene catheters without any sealing. We also describe a simple calibration method using a standard electrometer. The linearity of the ejected volume with respect to pulse duration and to applied pressure was maintained, as measured by the resistivity of ultrapure water after ejection of a saline solution. The method was used to analyse the inhibitory effect of glutamate on crayfish motoneurones with local and reversible applications of low-chloride saline and picrotoxin.
CNRS, Laboratoire Neurobiologie et Mouvements, Marseille, France.
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Marchand
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Pearlstein
D000818:Animals; D003400:Astacoidea / Q000502:physiology; D002138:Calibration; D002712:Chlorides / Q000494:pharmacology; D018698:Glutamic Acid / Q000008:administration & dosage / Q000494:pharmacology; D066298:In Vitro Techniques; D008845:Microinjections / Q000295:instrumentation*; D009046:Motor Neurons / Q000187:drug effects / Q000502:physiology; D011312:Pressure; D012965:Sodium Chloride; D010566:Virulence Factors, Bordetella / Q000008:administration & dosage / Q000494:pharmacology
D002712:Chlorides; D010566:Virulence Factors, Bordetella; D018698:Glutamic Acid; D012965:Sodium Chloride