Interleukin-10 induces interleukin-11 responsiveness in human myeloma cell lines.
Interleukin (IL)-6-dependent human myeloma cell lines (HMCL) can be reproducibly obtained from patients with multiple myeloma and terminal disease. The growth of some of these HMCL can also be supported by IL-11. We show that IL-11-responsive, but not -unresponsive, HMCL expressed the gene of human IL-11 receptor (IL-11R) and produced an autocrine IL-10. All HMCL expressed the IL-10 receptor. In addition, IL-10 induced IL-11R gene expression and conferred IL-11 responsiveness on unresponsive HMCL. The ability of HMCL to produce IL-10 was strictly correlated with the capacity of the original patient's myeloma cells to produce IL-10 or not, and with the presence or absence of IL-10 in the patient's plasma.
Institute for Molecular Genetics, CNRS, Montpellier, France.
Z Y
ZY
Lu
Z J
ZJ
Gu
X G
XG
Zhang
J
J
Wijdenes
P
P
Neddermann
J F
JF
Rossi
B
B
Klein
D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D016753:Interleukin-10 / Q000494:pharmacology*; D017370:Interleukin-11 / Q000494:pharmacology*; D053703:Interleukin-11 Receptor alpha Subunit; D009101:Multiple Myeloma / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012334:RNA, Neoplasm / Q000032:analysis; D018123:Receptors, Interleukin / Q000096:biosynthesis / Q000235:genetics; D053701:Receptors, Interleukin-10; D053702:Receptors, Interleukin-11
C508428:IL11RA protein, human; D017370:Interleukin-11; D053703:Interleukin-11 Receptor alpha Subunit; D012334:RNA, Neoplasm; D018123:Receptors, Interleukin; D053701:Receptors, Interleukin-10; D053702:Receptors, Interleukin-11; D016753:Interleukin-10