Characterization of proteins phosphorylated by the cAMP-dependent protein kinase of bovine heart mitochondria.
Characterization of two mitochondrial proteins of M(r) 42 and 18 kDa, respectively, phosphorylated by the cAMP-dependent protein kinase of bovine heart mitochondria (mtPKA), is presented. A 42 kDa protein is found to be loosely associated to complexes I, III and IV of the respiratory chain and complex V (ATP synthase) in the inner mitochondrial membrane. An 18 kDa protein is associated to complex I in the inner membrane and in a purified preparation of this complex where it can be phosphorylated by the isolated catalytic subunit of PKA.
Institute of Medical Biochemistry and Chemistry, CNR, University of Bari, Italy.
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Sardanelli
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Technikova-Dobrova
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D000251:Adenosine Triphosphatases / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002352:Carrier Proteins; D002417:Cattle; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism*; D003576:Electron Transport Complex IV / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D008565:Membrane Proteins / Q000378:metabolism; D008929:Mitochondria, Heart / Q000201:enzymology / Q000378:metabolism*; D025261:Mitochondrial Proton-Translocating ATPases; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000378:metabolism; D010750:Phosphoproteins / Q000096:biosynthesis*; D010766:Phosphorylation
D002352:Carrier Proteins; D008565:Membrane Proteins; D010750:Phosphoproteins; D000255:Adenosine Triphosphate; D016660:NAD(P)H Dehydrogenase (Quinone); D003576:Electron Transport Complex IV; D017868:Cyclic AMP-Dependent Protein Kinases; D000251:Adenosine Triphosphatases; D025261:Mitochondrial Proton-Translocating ATPases; C020439:oligomycin sensitivity-conferring protein