Amino acid sequence analysis of human S100A7 (psoriasin) by tandem mass spectrometry.
The Ca(2+)-binding proteins regulate a number of cellular and extracellular activities and deregulations of S100 gene expression are associated with several human diseases. For example, S100A7 is upregulated in psoriatic skin, implicating a link with psoriasis, a chronic inflammatory dermatosis. We purified human S100A7 and determined its protein sequence by tandem mass spectrometry and Edman microsequence analysis. Interestingly, a sequence comparison of S100A7 with all known human S100 proteins showed that S100A7 is the most divergent of all S100 proteins.
Bürgisser
D M
DM
Department of Pediatrics, University of Zurich, Switzerland.
Siegenthaler
G
G
Kuster
T
T
Hellman
U
U
Hunziker
P
P
Birchler
N
N
Heizmann
C W
CW
D000595:Amino Acid Sequence; D002135:Calcium-Binding Proteins / Q000737:chemistry / Q000235:genetics*; D003600:Cytosol / Q000737:chemistry; D006801:Humans; D013058:Mass Spectrometry; D008566:Membranes / Q000737:chemistry; D008969:Molecular Sequence Data; D015394:Molecular Structure; D011565:Psoriasis / Q000235:genetics; D000075023:S100 Calcium Binding Protein A7; D009418:S100 Proteins; D017386:Sequence Homology, Amino Acid
D002135:Calcium-Binding Proteins; D000075023:S100 Calcium Binding Protein A7; D009418:S100 Proteins; C089242:S100A7 protein, human