Expression of myotonic dystrophy protein kinase in biopsied muscles.
We present expression of myotonic dystrophy protein kinase (DM-PK) on biopsied muscles by immunocytochemistry using antibody against synthetic DM-PK peptide antigen. Immunolocalization of DM-PK was observed in neuromuscular junctions, muscle spindle, and sarcoplasm on both normal and DM muscles. DM-PK expression of sarcoplasm was present in adult normal and DM muscles. In Duchenne and Becker muscular dystrophies, DM-PK was intensively expressed in cytoplasm on immature regenerating fibers. DM-PK is initially produced in cytoplasm of regenerating fibers and migrates toward sarcoplasm with maturity of muscle cell.
School of Health Sciences, Sapporo Medical University, Japan.
N
N
Tachi
N
N
Kozuka
K
K
Ohya
S
S
Chiba
K
K
Kikuchi
D000328:Adult; D001706:Biopsy; D006801:Humans; D007150:Immunohistochemistry; D009132:Muscles / Q000201:enzymology* / Q000473:pathology; D009136:Muscular Dystrophies / Q000201:enzymology / Q000473:pathology; D009223:Myotonic Dystrophy / Q000201:enzymology* / Q000473:pathology; D065636:Myotonin-Protein Kinase; D017346:Protein Serine-Threonine Kinases / Q000032:analysis*
C585517:DMPK protein, human; D065636:Myotonin-Protein Kinase; D017346:Protein Serine-Threonine Kinases