Human ClpP protease: cDNA sequence, tissue-specific expression and chromosomal assignment of the gene.
We identified three overlapping human expressed sequence tags with significant homology to the E. coli ClpP amino sequence by screening the EMBL nucleotide database. With this sequence information we applied 5' and 3'-rapid amplification of cDNA ends (RACE) to amplify and sequence human clpP cDNA in two overlapping fragments. The open reading frame encodes a 277 amino acid long precursor polypeptide. Two ClpP specific motifs surrounding the active site residues are present and extensive homology to ClpP's from other organisms was observed. Northern blotting showed high relative expression levels of clpP mRNA in skeletal muscle, intermediate levels in heart, liver and pancreas, and low levels in brain, placenta, lung and kidney. By analysis of human/rodent cell hybrids the human clpP gene was assigned to chromosome 19.
Center for Medical Molecular Biology, Aarhus University Hospital, Denmark.
P
P
Bross
B S
BS
Andresen
I
I
Knudsen
T A
TA
Kruse
N
N
Gregersen
D000251:Adenosine Triphosphatases; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D002888:Chromosomes, Human, Pair 19; D018076:DNA, Complementary; D049071:Endopeptidase Clp; D006801:Humans; D006822:Hybrid Cells; D008969:Molecular Sequence Data; D016376:Oligonucleotides, Antisense; D012377:Rodentia; D017386:Sequence Homology, Amino Acid; D016324:Sequence Tagged Sites; D012697:Serine Endopeptidases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
D018076:DNA, Complementary; D016376:Oligonucleotides, Antisense; D012697:Serine Endopeptidases; D049071:Endopeptidase Clp; D000251:Adenosine Triphosphatases