Relaxation enhancement of the transverse magnetization of water protons in paramagnetic suspensions of red blood cells.
The enhancement of the water proton transverse relaxation, delta R2, brought about by a difference between intra and extracellular paramagnetic susceptibilities in a suspension of red blood cells (RBC) has been evaluated both experimentally and theoretically in terms of (i) the refocusing interval, delta 180, of a CPMG pulse sequence, (ii) the difference in paramagnetic susceptibility, and (iii) the shape of the cell surface. At a hematocrit of 45, the increase in the relaxation enhancement, delta R2, with increasing delta 180, was a factor of two greater for the naturally biconcave RBC, than for the quasi-spherical RBC in hypotonic suspensions. This difference could be modeled in terms of a transmembrane correlation time, tau = 5.5 ms, across an RBC surface characterized by a demagnetizing factor which differs by 0.13 from that of a sphere. The increase in delta R2 with increasing magnetization difference between the RBC and its surroundings was found to be marginally less than quadratic, both experimentally and from the model.
Department of Biomedical Engineering, University of Alberta, Edmonton, Canada.
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Allen
D000818:Animals; D003198:Computer Simulation; D004285:Dogs; D004912:Erythrocytes; D006400:Hematocrit; D008280:Magnetics; D014867:Water
D014867:Water