Detection of membrane and soluble interleukin-6 receptor in lymphoid malignancies.
We studied the membrane expression of the gp80 chain of IL-6 receptor (IL-6R) by quantitative flow cytometry in chronic lymphocytic leukaemia (CLL) and leukaemic centrocytic lymphoma using a panel of seven monoclonal antibodies. IL-6R was detected in 18/26 CLL cases and 4/7 lymphoma cases, with a mean antigen density < 3000 molecules/cell. Multiple labelling experiments confirmed the IL-6R expression by neoplastic cells. Specific mRNA was found by RT-PCR in neoplastic cells. A specific ELISA test was designed using two anti-IL-6 receptor MAbs to measure the serum soluble IL-6R (sIL-6R) in CLL (n = 48). B-cell non-Hodgkin's lymphoma (NHL; n = 40), and monoclonal gammopathy (MG; n = 32). SIL-6R was higher in CLL (170 +/- 12.6 ng/ml) in NHL (160 +/- 12 ng/ml) and MG patients (183 +/- 23 ng/ml) than in age-matched controls (100 +/- 5.6 ng/ml; P < 0.001) and higher in high-grade than low-grade NHL. No correlation was noted with a previous treatment. Among CLL cases the patients classified as stage B according to the Binet's staging of the disease had the highest sIL-6R values, thus suggesting a link with tumour cell mass.
Service des Maladies du Sang, C.H.U., Montpellier, France.
T
T
Lavabre-Bertrand
C
C
Exbrayat
J
J
Liautard
J P
JP
Gaillard
P P
PP
Baskevitch
N
N
Poujol
C
C
Duperray
P
P
Bourquard
J
J
Brochier
D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000032:analysis* / Q000235:genetics; D001483:Base Sequence; D002462:Cell Membrane / Q000276:immunology; D017931:DNA Primers; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000097:blood / Q000276:immunology* / Q000473:pathology; D008228:Lymphoma, Non-Hodgkin / Q000097:blood / Q000276:immunology* / Q000473:pathology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D018123:Receptors, Interleukin / Q000032:analysis* / Q000235:genetics; D019947:Receptors, Interleukin-6
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D017931:DNA Primers; D018123:Receptors, Interleukin; D019947:Receptors, Interleukin-6