Evaluation by polymerase chain reaction on the effect of beta-propiolactone and binary ethyleneimine on DNA.
Inactivating treatments for viruses such as pasteurization or alkylation by beta-propiolactone or binary ethyleneimine were tested for their capacity to modify nucleic acids. The modification of a nucleic acid was measured as the decrease in spot intensity in Southern blots after polymerase chain reaction (PCR) amplification. The inactivating treatments were applied to cellular and viral genomic material from a human lymphoblastoid cell line immortalized by Epstein Barr Virus (EBV), which produced a monoclonal antibody. Pasteurization did not modify the ability to amplify and detect cellular or viral DNA. Binary ethyleneimine strongly reduced the amount of detectable DNA and beta-propiolactone under particular conditions of incubation abolished all trace of DNA.
Laboratoire Français du fractionnement et des Biotechnologies, LFB, Les Ulis, France.
C
C
Groseil
P
P
Guerin
P
P
Adamowicz
D000477:Alkylating Agents / Q000494:pharmacology*; D001388:Aziridines / Q000494:pharmacology*; D001483:Base Sequence; D002460:Cell Line; D004247:DNA / Q000187:drug effects*; D004249:DNA Damage; D017931:DNA Primers; D015342:DNA Probes; D006361:Heating; D004854:Herpesvirus 4, Human / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011420:Propiolactone / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D000477:Alkylating Agents; D001388:Aziridines; D017931:DNA Primers; D015342:DNA Probes; C033132:aziridine; D011420:Propiolactone; D004247:DNA