Isolation and characterization of a cDNA encoding the alpha' subunit of human cone cGMP-phosphodiesterase.
A human cDNA (alpha-PDE) encoding the alpha' catalytic subunit of cone photoreceptor cGMP-phosphodiesterase has been isolated and characterized. The nucleotide sequence of 2980 bp contains an open reading frame encoding an 859-amino-acid (aa) protein with a calculated molecular mass of 99 kDa. Northern blot analysis of human retinal mRNA hybridized with the alpha'-PDE cDNA revealed a signal corresponding to a 3.2-kb transcript. Comparison of the deduced aa sequence of human cone alpha'-PDE with corresponding proteins isolated from bovine and chicken retinas shows 89 and 83% identity, respectively, and indicates that alpha'-PDE has been very well conserved in the evolutionary process. Human cone alpha'-PDE is highly homologous to rod alpha-PDE and beta-PDE, suggesting that these proteins may have a close phylogenetic relationship.
Jules Stein Eye Institute, UCLA School of Medicine 90095, USA.
A S
AS
Viczian
N I
NI
Piriev
D B
DB
Farber
D015106:3',5'-Cyclic-GMP Phosphodiesterases / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002645:Chickens; D018076:DNA, Complementary / Q000235:genetics; D015870:Gene Expression; D005796:Genes; D006801:Humans; D007527:Isoenzymes / Q000235:genetics; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics; D017949:Retinal Cone Photoreceptor Cells / Q000201:enzymology*; D017948:Retinal Rod Photoreceptor Cells / Q000201:enzymology; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic
D018076:DNA, Complementary; D007527:Isoenzymes; D012333:RNA, Messenger; D015106:3',5'-Cyclic-GMP Phosphodiesterases