Preparation, manipulation, and pulse strategy for one-dimensional pulsed-field gel electrophoresis (ODPFGE).
The underlying principles for zero-integrated-field electrophoresis (ZIFE) pulses and more general forward-biased pulse schemes are reviewed for one-dimensional pulsed-field gel electrophoresis (ODPFGE) separations of large DNA molecules. Detailed descriptions of materials, preparation protocols, hardware requirements, and procedures are given. A variety of gel pictures for known yeast DNA markers are shown.
Xerox Research Centre of Canada, Mississauga, Ontario, Canada.
J
J
Noolandi
C
C
Turmel
D000465:Algorithms; D015139:Blotting, Southern; D002021:Buffers; D002175:Candida / Q000235:genetics; D002176:Candida albicans / Q000235:genetics; D002875:Chromosomes / Q000737:chemistry; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D004271:DNA, Fungal / Q000737:chemistry; D004560:Electricity; D016521:Electrophoresis, Gel, Pulsed-Field / Q000295:instrumentation / Q000379:methods*; D012441:Saccharomyces cerevisiae / Q000235:genetics
D002021:Buffers; D004271:DNA, Fungal; D004247:DNA