High-resolution comparative mapping of the proximal region of the mouse X chromosome.
The murine homologues of the loci for McLeod syndrome (XK), Dent's disease (CICN5), and synaptophysin (SYP) have been mapped to the proximal region of the mouse X chromosome and positioned with respect to other conserved loci in this region using a total of 948 progeny from two separate Mus musculus x Mus spretus backcrosses. In the mouse, the order of loci and evolutionary breakpoints (EB) has been established as centromere-(DXWas70, DXHXF34h)-EB-Clcn5-(Syp, DXMit55, DXMit26)-Tfe3-Gata1-EB-Xk-Cybb-telomere. In the proximal region of the human X chromosome short arm, the position of evolutionary breakpoints with respect to key loci has been established as DMD-EB-XK-PFC-EB-GATA1-C1CN5-EB-DXS1272E-ALAS2-E B-DXF34-centromere. These data have enabled us to construct a high-resolution genetic map for the approximately 3-cM interval between DXWas70 and Cybb on the mouse X chromosome, which encompasses 10 loci. This detailed map demonstrates the power of high-resolution genetic mapping in the mouse as a means of determining locus order in a small chromosomal region and of providing an accurate framework for the construction of physical maps.
Genetics Division, MRC Radiobiology Unit, Chilton, Oxon, United Kingdom.
H J
HJ
Blair
M
M
Ho
A P
AP
Monaco
S
S
Fisher
I W
IW
Craig
Y
Y
Boyd
D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D019143:Evolution, Molecular; D005784:Gene Amplification; D005819:Genetic Markers; D014644:Genetic Variation; D006239:Haplotypes; D006801:Humans; D051379:Mice / Q000235:genetics*; D018895:Microsatellite Repeats; D008969:Molecular Sequence Data; D009115:Muridae / Q000235:genetics; D012150:Polymorphism, Restriction Fragment Length; D013045:Species Specificity; D014960:X Chromosome
D005819:Genetic Markers