Angiotensin and bradykinin metabolism by peptidases identified in skeletal muscle.
We have identified angiotensin-converting enzyme, neutral endopeptidase-24.11, and aminopeptidase M in a purified glycoprotein fraction of rabbit skeletal muscle membranes. The identification was based on substrate specificity and sensitivity to selective inhibitors. Angiotensin I metabolism was due to angiotensin-converting enzyme-mediated conversion to angiotensin II and neutral endopeptidase-24.11-mediated conversion to angiotensin(1-7). Bradykinin was degraded by angiotensin-converting enzyme and neutral endopeptidase-24.11; angiotensin II by neutral endopeptidase-24.11; and angiotensin III by neutral endopeptidase-24.11 and aminopeptidase M. Thus, the effects of angiotensins and kinins on skeletal muscle blood flow and metabolism may be regulated by local angiotensin-converting enzyme, neutral endopeptidase-24.11, and aminopeptidase M.
Ward
P E
PE
Department of Physiology, Ohio State University, Columbus 43210, USA.
Russell
J S
JS
Vaghy
P L
PL
D000626:Aminopeptidases / Q000378:metabolism; D000803:Angiotensin I / Q000378:metabolism; D000804:Angiotensin II / Q000378:metabolism; D000805:Angiotensin III / Q000378:metabolism; D000809:Angiotensins / Q000378:metabolism*; D000818:Animals; D001920:Bradykinin / Q000378:metabolism*; D066298:In Vitro Techniques; D063208:Methionyl Aminopeptidases; D018482:Muscle, Skeletal / Q000378:metabolism*; D015260:Neprilysin / Q000378:metabolism; D010447:Peptide Hydrolases / Q000378:metabolism*; D007703:Peptidyl-Dipeptidase A / Q000378:metabolism; D011817:Rabbits
D000809:Angiotensins; D000804:Angiotensin II; D000805:Angiotensin III; D000803:Angiotensin I; D010447:Peptide Hydrolases; D000626:Aminopeptidases; D063208:Methionyl Aminopeptidases; D007703:Peptidyl-Dipeptidase A; D015260:Neprilysin; D001920:Bradykinin