High-performance liquid chromatographic determination of irinotecan (CPT-11) and its active metabolite (SN-38) in human plasma.
A simplified method for the simultaneous determination of irinotecan (CPT-11, I) and its active metabolite (SN-38, II) in human plasma by high-performance liquid chromatography (HPLC) with fluorescence detection has been developed. Following the addition of the internal standard (I.S.) camptothecin, the drugs were extracted from plasma using methanol. The average extraction efficiencies were 87% for I, 90% for II and 90% for the I.S. Chromatography was performed using a TSK gel ODS-80Ts column, monitored at 556 nm (excitation wavelength, 380 nm) and the mobile phase was acetonitrile-50 mM disodium hydrogen phosphate (28:72) containing 5 mM heptanesulphonate (pH 3.0). The linear quantitation ranges for I and II were 30-2000 and 1-30 ng/ml, respectively.
Second Department of Internal Medicine, Hiroshima University School of Medicine, Japan.
H
H
Sumiyoshi
Y
Y
Fujiwara
T
T
Ohune
N
N
Yamaoka
K
K
Tamura
M
M
Yamakido
D000972:Antineoplastic Agents, Phytogenic / Q000097:blood* / Q000493:pharmacokinetics; D002166:Camptothecin / Q000031:analogs & derivatives* / Q000097:blood / Q000493:pharmacokinetics; D018288:Carcinoma, Small Cell / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007202:Indicators and Reagents; D007275:Injections, Intravenous; D000077146:Irinotecan; D008175:Lung Neoplasms / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D013055:Spectrophotometry, Infrared
D000972:Antineoplastic Agents, Phytogenic; D007202:Indicators and Reagents; D000077146:Irinotecan; D002166:Camptothecin