A sublocus of the multicopy microsatellite marker CMS1 maps proximal to spinal muscular atrophy (SMA) as shown by recombinant analysis.
The critical region containing the spinal muscular atrophy (SMA) gene is flanked by the 5q11-q13 markers, D5S435 and D5S557, as determined by linkage analysis. Here we present the results of an analysis of a Dutch SMA family with the multicopy microsatellite marker CMS1. A crossover is revealed in the critical SMA region. We conclude that at least one of the CMS1 subloci maps proximal to the SMA gene. This reduces the minimal SMA region from approximately 1.4 Mb to 600-700 kb.
Department of Medical Genetics, University of Groningen, The Netherlands.
G
G
van der Steege
J M
JM
Cobben
J
J
Osinga
H
H
Scheffer
G J
GJ
van Ommen
C H
CH
Buys
D002874:Chromosome Mapping; D004276:DNA, Satellite / Q000032:analysis*; D005260:Female; D006801:Humans; D008297:Male; D009134:Muscular Atrophy, Spinal / Q000235:genetics*; D016133:Polymerase Chain Reaction
D004276:DNA, Satellite