Detection of additional restriction fragment length polymorphisms among the weakly virulent (nonaggressive) and highly virulent (aggressive) isolates of Leptosphaeria maculans.
Isolates of Leptosphaeria maculans were analyzed for their genetic relatedness based on DNA restriction fragment length polymorphisms (RFLPs), employing as Southern hybridization probes a combination of heat shock responsive genes (hsp70 and hsp80 from Neurospora crassa), the cutinase gene of Magnaporthe grisea, and cloned genomic DNA sequences from a virulent strain. Southern hybridization analysis revealed a high frequency of DNA polymorphism. Restriction fragments generated by each enzyme-probe combination resulted in distinct banding patterns, clearly separating the isolates into two groups. The cutinase gene probe did not reveal any polymorphisms. Although the majority of the probes used displayed RFLP profiles unique to each group, a nonaggressive isolate, LmA, showed additional genetic characteristics in common with the virulent pathotype.
Department of Biological Sciences, University of Calgary, Canada.
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Patterson
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M
Kapoor
D001203:Ascomycota / Q000235:genetics* / Q000472:pathogenicity; D015342:DNA Probes; D004271:DNA, Fungal / Q000235:genetics*; D006360:Heat-Shock Proteins; D012150:Polymorphism, Restriction Fragment Length; D014774:Virulence / Q000235:genetics
D015342:DNA Probes; D004271:DNA, Fungal; D006360:Heat-Shock Proteins