[The effect of TNF-alpha (tumor necrosis factor-alpha) on progesterone synthesis in vitro by cells from ovarian follicular fluid in women].
Follicular fluid was obtained through the ovarian follicle puncture during laparotomy in seven women. Follicular cells were cultured for 66 hours in the presence of TNF-alpha or lipopolysaccharides. Concentration of FSH, LH and prolactin in follicular fluid and progesterone levels in culture medium were estimated. TNF-alpha (100 ng/ml medium) diminished progesterone production in 6 hours tissue culture but reduced its concentration in 18 and 42 hours culture in comparison to the controls. Correlation was found between FSH level in follicular fluid and percentage of progesterone concentration change in experiments with TNF-alpha 100 mg/ml up to 42 hours and with TNF-alpha 500 ng/ml up to 18 hours. Lipopolysaccharides (10 micrograms/ml) did not influence progesterone production in the tissue culture.
Putowski
L
L
II Kliniki Ginekologii Operacyjnej Akademii Medycznej, Lublinie.
Kotarski
J
J
D000293:Adolescent; D000328:Adult; D002478:Cells, Cultured; D005260:Female; D005640:Follicle Stimulating Hormone / Q000032:analysis; D015571:Follicular Fluid / Q000737:chemistry*; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology; D007986:Luteinizing Hormone / Q000032:analysis; D006080:Ovarian Follicle / Q000187:drug effects* / Q000378:metabolism; D011374:Progesterone / Q000096:biosynthesis*; D011388:Prolactin / Q000032:analysis; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D011374:Progesterone; D011388:Prolactin; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone
Wpływ TNF-alpha (tumor necrosis factor-alfa) na synteze progesteronu in vitro przez komórki z płynu pecherzyka jajnika kobiety.