Superoxide dismutase in SAS human tongue carcinoma cell line is a factor defining invasiveness and cell motility.
This article describes an apparent inverse relationship between cell motility and intracellular Cu-Zn superoxide dismutase (SOD) activity of two human squamous carcinoma-derived clones, SAS-H1 with high invasiveness and SAS-L1 with low invasiveness. Clone SAS-H1 exhibited significantly greater motility than SAS-L1 but had significantly lower levels of intracellular Cu-ZnSOD than SAS-L1 cells. We then transfected Cu-ZnSOD antisense cDNA into SAS-L1 to reduce the intracellular Cu-ZnSOD activity. Antisense cDNA transfected SAS-L-AS clones had lower Cu-ZnSOD activity than control vector-transfected SAS-L-Neo clones, and this was associated with increased motility. Invasiveness of SAS-H1 and SAS-L-AS1 was enhanced by superoxide treatment, while the invasiveness of SAS-L1 was unaffected. These findings indicate that intracellular SOD is involved in cell motility by virtue of its action in scavenging superoxide in the cells.
Fourth Department of Internal Medicine, Sapporo Medical University School of Medicine, Hokkaido, Japan.
H
H
Muramatsu
K
K
Kogawa
M
M
Tanaka
K
K
Okumura
Y
Y
Nishihori
K
K
Koike
T
T
Kuga
Y
Y
Niitsu
D001483:Base Sequence; D002294:Carcinoma, Squamous Cell / Q000201:enzymology* / Q000473:pathology*; D002465:Cell Movement; D017931:DNA Primers / Q000737:chemistry; D016373:DNA, Antisense; D006801:Humans; D008969:Molecular Sequence Data; D009361:Neoplasm Invasiveness; D013482:Superoxide Dismutase / Q000378:metabolism*; D013481:Superoxides / Q000378:metabolism / Q000494:pharmacology; D014062:Tongue Neoplasms / Q000201:enzymology* / Q000473:pathology*; D014407:Tumor Cells, Cultured
D017931:DNA Primers; D016373:DNA, Antisense; D013481:Superoxides; D013482:Superoxide Dismutase