A point mutation in the mitochondrial DNA of diabetes-prone BHE/cdb rats.
Mitochondrial DNA was extracted from hepatic tissue of 50- and 300-day-old male BHE/cdb and Sprague-Dawley rats. The complete gene for the F0ATPase subunits 6 and 8 was sequenced. Four nucleotide substitutions were found: three of the substitutions were silent; the other substitution at position 523 was not. Its codon dictates the substitution of asparagine for aspartic acid in a critical location (in the polar pocket) of the F0ATPase. It is possible that this point mutation may explain previously reported decreases in ATP synthesis efficiency in BHE/cdb rats compared to Sprague-Dawley rats.
Mathews
C E
CE
Department of Foods and Nutrition, University of Georgia, Athens 30602, USA.
McGraw
R A
RA
Berdanier
C D
CD
D000375:Aging / Q000378:metabolism; D000818:Animals; D001483:Base Sequence; D004272:DNA, Mitochondrial / Q000235:genetics*; D003924:Diabetes Mellitus, Type 2 / Q000201:enzymology / Q000235:genetics*; D004195:Disease Models, Animal; D004734:Energy Metabolism; D008297:Male; D008969:Molecular Sequence Data; D017354:Point Mutation; D006180:Proton-Translocating ATPases / Q000172:deficiency / Q000235:genetics*; D051381:Rats; D011922:Rats, Mutant Strains / Q000235:genetics*; D017207:Rats, Sprague-Dawley; D012689:Sequence Homology, Nucleic Acid
D004272:DNA, Mitochondrial; D006180:Proton-Translocating ATPases