Convenient desktop-scale production of the extracellular domain of the human growth hormone receptor by an insect-baculovirus secretion system using a protein-free culture.
Expression of a gene encoding the extracellular domain of the human growth hormone receptor (hGHR-ED) inserted into the genome of Autographa californica nuclear polyhedrosis virus was done using a desktop-scale spinner culture. Spodoptera frugiperda 9 (Sf9) cells infected with the recombinant virus secreted a protein with hGH-binding activity into the medium. Oxygen supplementation was required for high level secretion of the product. The highest cell production capability was estimated at more than 15 mg hGHR-ED/liter of culture. A protein-free medium supported the production similar to that obtained in traditional serum-containing media. This spinner culture system is simple to operate, and does not require expert knowledge of culture techniques.
Protein Engineering Research Institute, Osaka, Japan.
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A
Asakura
M
M
Minami
Y
Y
Ota
D000818:Animals; D016367:Baculoviridae; D002460:Cell Line; D003001:Cloning, Molecular / Q000379:methods*; D006801:Humans; D011986:Receptors, Somatotropin / Q000096:biosynthesis / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D018411:Spodoptera
D011986:Receptors, Somatotropin; D011993:Recombinant Fusion Proteins