Cloning and nucleotide sequence of a leaf ferredoxin-nitrite reductase cDNA of rice.
A ferredoxin-nitrite reductase (EC 1.7.7.1) cDNA was isolated and sequenced from a lambda gt 11 cDNA library constructed from nitrate-induced greening shoots of rice (Oryza sativa L.) seedlings. The nucleotide sequence of the cDNA clone contains an open reading frame of 1788 nucleotides. There exists a strong bias for the third codon usage of G/C (95.5%) as in the case of the maize enzyme. The deduced amino acid sequence shows an overall homology to the maize (81%) and the dicot enzymes (70-74%), suggesting that the primary structure of ferredoxin-nitrite reductase is highly conserved in higher plants.
Terada
Y
Y
Research Institute for Food Science, Kyoto University, Japan.
Aoki
H
H
Tanaka
T
T
Morikawa
H
H
Ida
S
S
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary; D050900:Ferredoxin-Nitrite Reductase; D008969:Molecular Sequence Data; D009572:Nitrite Reductases / Q000235:genetics*; D012275:Oryza / Q000201:enzymology / Q000235:genetics*; D018515:Plant Leaves / Q000201:enzymology*; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D009572:Nitrite Reductases; D050900:Ferredoxin-Nitrite Reductase