Inhibition of angiogenesis by thrombospondin-2.
To assess the ability of proteins of the thrombospondin family to inhibit angiogenesis, recombinant murine thrombospondin-2, bovine thrombospondin-2/CISP and thrombospondin-5/COMP were purified and tested for ability to block the migration of capillary endothelial cells towards a variety of inducers and to inhibit neovascularization induced in the rat cornea. Both preparations of thrombospondin-2 were active inhibitors in vitro and in vivo whereas thrombospondin-5/COMP was inactive. These results define thrombospondin-2 as a newly identified naturally occurring inhibitor of angiogenesis and suggest that the properdin-like type 1 modules that it shares with antiangiogenic thrombospondin-1 and are missing in thrombospondin-5/COMP could contribute to this activity.
Volpert
O V
OV
Department of Microbiology-Immunology, Northwestern University Medical School, Chicago, IL, USA.
Tolsma
S S
SS
Pellerin
S
S
Feige
J J
JJ
Chen
H
H
Mosher
D F
DF
Bouck
N
N
D000311:Adrenal Glands / Q000098:blood supply / Q000187:drug effects; D000818:Animals; D002417:Cattle; D015815:Cell Adhesion Molecules / Q000494:pharmacology* / Q000502:physiology; D002465:Cell Movement / Q000187:drug effects; D016510:Corneal Neovascularization / Q000517:prevention & control; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects; D066298:In Vitro Techniques; D008562:Membrane Glycoproteins / Q000494:pharmacology* / Q000502:physiology; D051379:Mice; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D051381:Rats; D011994:Recombinant Proteins / Q000494:pharmacology; D019699:Thrombospondins
D015815:Cell Adhesion Molecules; D008562:Membrane Glycoproteins; D011994:Recombinant Proteins; D019699:Thrombospondins