Laser-excited fluorescence studies of mitochondrial function in saponin-skinned skeletal muscle fibers of patients with chronic progressive external ophthalmoplegia.
The functional behavior of mitochondria in skeletal muscle of patients with chronic progressive external ophthalmoplegia was studied by laser-excited fluorescence measurements of NAD(P)H and flavoproteins in saponin-skinned fibers. Variations in the mitochondrial content and the presence of partially respiratory chain-inhibited mitochondria can be detected using this novel method.
Klinik für Neurologie, Universitätsklinikum Magdeburg, Germany.
K
K
Winkler
A V
AV
Kuznetsov
H
H
Lins
E
E
Kirches
P
P
von Bossanyi
K
K
Dietzmann
B
B
Frank
H
H
Feistner
W S
WS
Kunz
D004272:DNA, Mitochondrial / Q000235:genetics; D004579:Electron Transport; D005260:Female; D005420:Flavoproteins / Q000378:metabolism; D005453:Fluorescence; D006801:Humans; D007834:Lasers; D008875:Middle Aged; D008931:Mitochondria, Muscle / Q000201:enzymology / Q000378:metabolism*; D018485:Muscle Fibers, Skeletal / Q000378:metabolism*; D009249:NADP / Q000378:metabolism; D017246:Ophthalmoplegia, Chronic Progressive External / Q000378:metabolism*; D010084:Oxidation-Reduction; D010101:Oxygen Consumption; D012503:Saponins
D004272:DNA, Mitochondrial; D005420:Flavoproteins; D012503:Saponins; D009249:NADP