Arabidopsis mutants deficient in T-DNA integration.
Arabidopsis thaliana mutants originally isolated as hypersensitive to irradiation were screened for the ability to be transformed by Agrobacterium transferred DNA (T-DNA). One of four UV-hypersensitive mutants and one of two gamma-hypersensitive mutants tested showed a significant reduction in the frequency of stable transformants compared with radioresistant controls. In a transient assay for T-DNA transfer independent of genomic integration, both mutant lines took up and expressed T-DNA as efficiently as parental lines. These lines are therefore deficient specifically in stable T-DNA integration and thus provide direct evidence for the role of a plant function in that process. As radiation hypersensitivity suggests a deficiency in repair of DNA damage, that plant function may be one that is also involved in DNA repair, possibly, from other evidence, in repair of double-strand DNA breaks.
Sonti
R V
RV
Department of Biology, Massachusetts Institute of Technology, Cambridge 02139, USA.
Chiurazzi
M
M
Wong
D
D
Davies
C S
CS
Harlow
G R
GR
Mount
D W
DW
Signer
E R
ER
D016960:Agrobacterium tumefaciens / Q000235:genetics; D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism / Q000528:radiation effects; D046508:Culture Techniques; D004260:DNA Repair; D004269:DNA, Bacterial / Q000235:genetics*; D009154:Mutation; D018517:Plant Roots / Q000235:genetics / Q000254:growth & development; D018520:Plant Shoots / Q000235:genetics / Q000254:growth & development; D011836:Radiation Tolerance; D014170:Transformation, Genetic; D014466:Ultraviolet Rays / Q000009:adverse effects
D004269:DNA, Bacterial; C036483:T-DNA