In vivo administration of IL-1 beta accelerates silk ligature-induced alveolar bone resorption in rats.
The effects of recombinant human interleukin-1 beta (rhIL-1 beta) on alveolar bone resorptive activity in rats were examined. Continuous administration of rhIL-1 beta or phosphate-buffered saline (PBS) was given via osmotic pumps for 3, 7 and 14 days to rats with silk ligatures around second maxillary molars. Other animals without ligatures received insertion of pumps containing rhIL-1 beta or remained untreated. Sections were subject to three different stains:--hematoxylin and eosin (H-E) for histology, acid phosphatase (ACPase) activity for osteoclast detection, and immunohistochemistry using anti-rat monocyte/macrophage monoclonal antibody (ED 1). In addition, body weight, plasma calcium and phosphorus levels were monitored. The mean body weight of rats receiving rhIL-1 beta was significantly lower (P < 0.05 to P < 0.01) compared with untreated rats throughout the experimental period. On Day 7, plasma calcium and phosphorus levels were significantly lower in rats receiving rhIL-1 beta than in rats receiving PBS only (P < 0.05). Sections revealed a moderate inflammatory cell infiltrate reaching near the alveolar crest in both groups with ligatures on Day 3. Only rats receiving rhIL-1 beta exhibited enhancement of inflammatory cell invasion on Days 7 and 14. In rats receiving rhIL-1 beta with ligatures, numerous resorption lacunae containing ACPase-positive multinucleated giant cells (MNGCs), coinciding with ED1-positive cells, were located on the mesial side of the septum where extensive bone resorption had occurred throughout the experimental period. In animals receiving rhIL-1 beta without ligatures, compared with untreated rats, increased ACPase-positive cells were observed on the mesial side of the septum on Day 3. In animals receiving PBS only, a few ACPase-positive cells were observed confined to the mesial regions where slight bone resorption occurred on Days 7 and 14. These results indicate that the administration of rhIL-1 beta accelerated alveolar bone destruction in ligature-induced periodontal tissue inflammation over a two-week period.
Koide
M
M
Department of Periodontology, Niigata University School of Dentistry, Japan.
Suda
S
S
Saitoh
S
S
Ofuji
Y
Y
Suzuki
T
T
Yoshie
H
H
Takai
M
M
Ono
Y
Y
Taniguchi
Y
Y
Hara
K
K
D000135:Acid Phosphatase; D016301:Alveolar Bone Loss / Q000209:etiology* / Q000473:pathology; D000818:Animals; D000911:Antibodies, Monoclonal; D001835:Body Weight; D001862:Bone Resorption / Q000209:etiology / Q000473:pathology; D002118:Calcium / Q000097:blood; D004396:Coloring Agents; D015726:Giant Cells / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D007260:Infusion Pumps; D019476:Insect Proteins; D007375:Interleukin-1 / Q000008:administration & dosage / Q000009:adverse effects*; D008026:Ligation; D008264:Macrophages / Q000473:pathology; D008297:Male; D008439:Maxillary Diseases / Q000209:etiology; D008963:Molar; D009000:Monocytes / Q000473:pathology; D010010:Osteoclasts / Q000473:pathology; D010518:Periodontitis / Q000209:etiology / Q000473:pathology; D010758:Phosphorus / Q000097:blood; D011506:Proteins; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins; D047011:Silk; D013537:Sutures
D000911:Antibodies, Monoclonal; D004396:Coloring Agents; D019476:Insect Proteins; D007375:Interleukin-1; D011506:Proteins; D011994:Recombinant Proteins; D047011:Silk; D010758:Phosphorus; D000135:Acid Phosphatase; D002118:Calcium