Modification of the fatty acid composition of Escherichia coli by coexpression of a plant acyl-acyl carrier protein desaturase and ferredoxin.
Expression of a plant delta 6-palmitoyl (16:0)-acyl carrier protein desaturase in Escherichia coli resulted in the accumulation of the novel monounsaturated fatty acids delta 6-hexadecenoic acid (16:1 delta 6) and delta 8-octadecenoic acid. Amounts of 16:1 delta 6 accumulated by E. coli were increased more than twofold by the expression of a plant ferredoxin together with the delta 6-16:0-acyl carrier protein desaturase.
Biology Department, Brookhaven National Laboratory, Upton, New York 11973, USA.
E B
EB
Cahoon
L A
LA
Mills
J
J
Shanklin
D001483:Base Sequence; D004926:Escherichia coli / Q000737:chemistry / Q000378:metabolism*; D005227:Fatty Acids / Q000032:analysis / Q000378:metabolism*; D005288:Ferredoxins / Q000502:physiology*; D006899:Mixed Function Oxygenases / Q000502:physiology*; D008969:Molecular Sequence Data; D010944:Plants / Q000378:metabolism*
D005227:Fatty Acids; D005288:Ferredoxins; D006899:Mixed Function Oxygenases; C018747:acyl-(acyl-carrier-protein)desaturase