Isolectins from seeds of Artocarpus lakoocha.
Two isolectins (ALA-I and ALA-II), were isolated from seed extracts of Artocarpus lakoocha by anion exchange chromatography on Q-Sepharose fast flow columns at pH 8.5 and 8.0 ALA-I was unbound to the column at pH 8.5 and moved towards the cathode in non-denaturing polyacrylamide gel electrophoresis, whereas ALA-II possessed opposite properties. The two A. lakoocha agglutinins appeared to be composed of two dissimilar subunits (alpha and beta of M(r) 14,000 and 17,200) bound non-covalently. The isolectins possessed several similar properties including: blood type agglutination; pH optimum; pH and temp stability; as well as binding specificity towards asialomucins.
Department of Biochemistry, Faculty of Medicine, Khon Kaen University, Thailand.
S
S
Wongkham
C
C
Wongkham
P
P
Boonsiri
S
S
Simasathiansophon
C
C
Trisonthi
K
K
Atisook
D000818:Animals; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002852:Chromatography, Ion Exchange; D010856:Columbidae; D006224:Cricetinae; D004591:Electrophoresis, Polyacrylamide Gel; D005777:Geese; D037102:Lectins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D051379:Mice; D037121:Plant Lectins; D010944:Plants / Q000737:chemistry*; D051381:Rats; D012639:Seeds / Q000737:chemistry
D037102:Lectins; D037121:Plant Lectins