Human REG family genes are tandemly ordered in a 95-kilobase region of chromosome 2p12.
Reg, first isolated from a rat regenerating islet cDNA library, is expressed in regenerating islet beta-cells. Recently, it has been revealed that Reg and Reg-related genes constitute a multigene family, the Reg family. In human, the four REG family genes, i.e., REG 1 alpha, REG 1 beta, REG-related sequence (RS) and HIP/PAP, have so far been isolated. In this study, we analyzed YAC clones containing the four genes and performed two-color FISH to determine the map order of the genes. The human REG family genes are tandemly ordered in the 95-kbp DNA region of chromosome 2p12 as follows: 2cen-HIP/PAP-RS-REG I alpha-REG I beta-ptel.
Department of Biochemistry, Tohoku University School of Medicine, Miyagi, Japan.
H
H
Miyashita
K
K
Nakagawara
M
M
Mori
Y
Y
Narushima
N
N
Noguchi
S
S
Moriizumi
S
S
Takasawa
H
H
Yonekura
T
T
Takeuchi
H
H
Okamoto
D000209:Acute-Phase Proteins / Q000235:genetics; D000951:Antigens, Neoplasm; D001483:Base Sequence; D014408:Biomarkers, Tumor; D002135:Calcium-Binding Proteins / Q000235:genetics*; D002874:Chromosome Mapping; D018244:Chromosomes, Artificial, Yeast; D002889:Chromosomes, Human, Pair 2; D015698:Genomic Library; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007515:Islets of Langerhans / Q000254:growth & development; D037181:Lectins, C-Type; D051847:Lithostathine; D008969:Molecular Sequence Data; D005810:Multigene Family; D009419:Nerve Tissue Proteins; D000073718:Pancreatitis-Associated Proteins
D000209:Acute-Phase Proteins; D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D002135:Calcium-Binding Proteins; D037181:Lectins, C-Type; D051847:Lithostathine; D009419:Nerve Tissue Proteins; D000073718:Pancreatitis-Associated Proteins; C497617:REG1A protein, human; C497618:REG1B protein, human; C000612141:REG3A protein, human