Biochemical approaches to the detection and characterization of membrane proteins carrying blood group determinants.
The investigation of red blood cell membrane proteins carrying blood group determinants mainly involves the use of specific antibodies--polyclonal human antibodies and both murine and human monoclonal antibodies--directed against blood group antigens. Other blood group specific reagents like lectins, also represent useful tools to identify membrane proteins. These reagents allowed the detection and, then the characterization of several red cell membrane components by using a series of methods based on their specific interaction with the corresponding antigen. Reagents and investigation methods are overviewed hereafter.
Centre Régional de Transfusion Sanguine, Nantes.
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D000911:Antibodies, Monoclonal / Q000276:immunology; D001789:Blood Group Antigens / Q000032:analysis* / Q000276:immunology; D001788:Blood Grouping and Crossmatching / Q000379:methods*; D004910:Erythrocyte Membrane / Q000737:chemistry*; D006801:Humans; D037102:Lectins; D008565:Membrane Proteins / Q000032:analysis* / Q000276:immunology
D000911:Antibodies, Monoclonal; D001789:Blood Group Antigens; D037102:Lectins; D008565:Membrane Proteins