Localization of the gene encoding the human heat shock cognate protein, HSP73, to chromosome 11.
The heat shock cognate protein HSP73 (or HSC70) is a member of the HSP70 multigene family. This protein has several functions, including binding to nascent polypeptides to facilitate correct folding and the uncoating of clathrin-coated vesicles. Analysis of somatic cell hybrids by two-dimensional protein gel electrophoresis revealed the presence of a 73-kDa protein in two hybrids containing human chromosomes 5, 6, 9, and 11 in common. Using Western blot analysis, we demonstrate that this protein is a member of the HSP70 family and, by Southern blot analysis, that the HSP73 gene is located on human chromosome 11. Fluorescence in situ hybridization further localized HSP73 to the region 11q23.3-q25. This region is involved in a number of genetic rearrangements and is associated with several well-characterized tumours.
Molecular Genetics and Development Laboratory, Monash University, Melbourne, Victoria, Australia.
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M
Tavaria
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T
Gabriele
R L
RL
Anderson
M E
ME
Mirault
E
E
Baker
G
G
Sutherland
I
I
Kola
D000818:Animals; D015139:Blotting, Southern; D015153:Blotting, Western; D016466:CHO Cells; D002352:Carrier Proteins / Q000096:biosynthesis / Q000235:genetics*; D002460:Cell Line; D002874:Chromosome Mapping; D002880:Chromosomes, Human, Pair 11; D002895:Chromosomes, Human, Pair 5; D002896:Chromosomes, Human, Pair 6; D002899:Chromosomes, Human, Pair 9; D006224:Cricetinae; D050883:HSC70 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics*; D015186:Hominidae / Q000235:genetics*; D006358:Hot Temperature; D006801:Humans; D006822:Hybrid Cells; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008970:Molecular Weight; D005810:Multigene Family
D002352:Carrier Proteins; D050883:HSC70 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; C494371:HSPA8 protein, human