Cytodiagnosis of parvovirus B19 infection from ascites fluid of hydrops fetalis: report of a case.
The cytologic changes in the smears of fetus ascites fluid with parvovirus B19 infections are described. Cytology revealed the ground-glass appearance of the nuclei having a perinuclear halo, and the nuclear chromatin was spread as a thin rim around the inclusion. Subsequently, monoclonal antibodies for parvovirus B19 were applied to identify the specific antigen in the Papanicolaou-destained specimens. Positive staining reaction products were found throughout the cytoplasm of cells. Similar phenomena were confirmed in paraffin-embedded tissue sections of many organs in autopsy materials. It is hoped that recognition of those virus-infected cells in the body cavity fluid of the fetus will be helpful in making a diagnosis of hydrops fetalis associated with parvovirus B19 infections of the pregnant woman.
Department of Pathology, National Akashi Hospital, Hyogo, Japan.
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Iwa
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Yutani
D000328:Adult; D000956:Antigens, Viral / Q000032:analysis; D001202:Ascitic Fluid / Q000821:virology*; D002467:Cell Nucleus / Q000473:pathology; D005260:Female; D005315:Fetal Diseases / Q000175:diagnosis* / Q000821:virology; D006801:Humans; D015160:Hydrops Fetalis / Q000209:etiology*; D007181:Inclusion Bodies, Viral / Q000473:pathology / Q000648:ultrastructure; D008854:Microscopy, Electron; D010322:Parvoviridae Infections / Q000175:diagnosis* / Q000821:virology; D016732:Parvovirus B19, Human / Q000302:isolation & purification*; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000175:diagnosis*
D000956:Antigens, Viral