Lupinus albus L. pathogenesis-related proteins that show similarity to PR-10 proteins.
We describe a group of three acidic proteins, pathogenesis-related (PR)-p16.5a, PR-p16.5b, and PR-p16.5c, that accumulate in the leaves of Lupinus albus L. cv Rio Maior plants when infected with the fungus Colletotrichum gloeosporioides Penz. These proteins co-migrate in sodium dodecyl sulfate-polyacrylamide gels as a single band of 16.5 kD, behaving as charge isomers, and are related to several members of the defense-related PR-10 protein family. Localization of the proteins was investigated by techniques of tissue printing and immunogold electron microscopy; they are predominantly associated with the vascular system and are localized extracellularly. The accumulation of PR-p16.5a, PR-p16.5b, and PR-p16.5c also seems to be induced by cucumber mosaic virus and by two forms of abiotic stress, salicylic acid and ultraviolet, suggesting a general defense role for these proteins.
Instituto de Tecnologia Química e Biológica, R. da Quinta Grande, Oeiras, Portugal.
M P
MP
Pinto
C P
CP
Ricardo
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies; D004591:Electrophoresis, Polyacrylamide Gel; D007887:Fabaceae / Q000378:metabolism* / Q000382:microbiology; D016253:Microscopy, Immunoelectron; D003904:Mitosporic Fungi / Q000472:pathogenicity; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010935:Plant Diseases; D018515:Plant Leaves / Q000648:ultrastructure; D010940:Plant Proteins / Q000096:biosynthesis* / Q000737:chemistry* / Q000302:isolation & purification; D010946:Plants, Medicinal; D051381:Rats / Q000276:immunology; D017386:Sequence Homology, Amino Acid
D000906:Antibodies; D010940:Plant Proteins; C053376:pathogenesis-related proteins, plant