A novel factor-dependent human myelodysplastic cell line, MDS92, contains haemopoietic cells of several lineages.
A novel long-term cultured interleukin (IL)-3-dependent human myelodysplastic cell line, MDS92, was shown to contain several myeloid-lineage cells such as neutrophils, macrophages, eosinophils, and a small number of megakaryocyte-lineage cells. Therefore this cell line possesses at least bipotential characteristics of myeloid- and megakaryocyte-lineages. Granulocyte colony-stimulating factor clearly promoted the neutrophil alkaline phosphatase activity of MDS92 cells. To the contrary, the incidence and growth of CD41-positive cells were hardly affected by the addition of IL-6, IL-11, c-mpl ligand (thrombopoietin, TPO) or erythropoietin. TPO slightly supported the growth of CD34-positive cell fraction, but not CD41-positive cell fraction of MDS92 cells in combination with IL-3 or Steel factor. This cell line will be a useful tool for the study of MDS stem cells, but the mechanism of commitment of differentiation in MDS stem cells remains unknown.
First Department of Internal Medicine, Fukui Medical School, Japan.
K
K
Tohyama
Y
Y
Tohyama
T
T
Nakayama
T
T
Ueda
T
T
Nakamura
Y
Y
Yoshida
D000469:Alkaline Phosphatase / Q000378:metabolism; D015703:Antigens, CD / Q000032:analysis; D015815:Cell Adhesion Molecules / Q000494:pharmacology; D002455:Cell Division; D002460:Cell Line; D019070:Cell Lineage; D004804:Eosinophils / Q000473:pathology; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology; D006412:Hematopoietic Stem Cells / Q000276:immunology / Q000473:pathology* / Q000502:physiology; D006801:Humans; D007377:Interleukin-3 / Q000494:pharmacology* / Q000502:physiology; D008264:Macrophages / Q000473:pathology; D008533:Megakaryocytes / Q000276:immunology / Q000473:pathology; D009190:Myelodysplastic Syndromes / Q000473:pathology*; D009504:Neutrophils / Q000187:drug effects / Q000201:enzymology / Q000473:pathology*; D019089:Stem Cell Factor / Q000494:pharmacology; D013926:Thrombopoietin / Q000494:pharmacology
D015703:Antigens, CD; D015815:Cell Adhesion Molecules; D007377:Interleukin-3; D019089:Stem Cell Factor; D016179:Granulocyte Colony-Stimulating Factor; D013926:Thrombopoietin; D000469:Alkaline Phosphatase