Determination of D-amino acids in serum from patients with renal dysfunction.
D-Ala and D-Ser were detected in the sera of both normal subjects and patients with renal dysfunction, and their concentrations were higher in the patients than in the normal subjects. A positive correlation between the concentration of D-Ala or D-Ser and that of creatinine (r = 0.733, p < 0.001 or r = 0.634, p < 0.001) or blood urea nitrogen (BUN) (r = 0.449, p < 0.05 or r = 0.629, p < 0.001) was observed in sera from 20 patients with renal dysfunction. The fraction (%D) of D-Ala in the total Ala in serum ([D/(D+L)] x 100) correlated well with the concentration of creatinine (r = 0.811, p < 0.001), suggesting that it is a candidate as a marker for renal proximal tubular dysfunction. The correlations of %D of Ser with creatinine and BUN levels were 0.796 (p < 0.001) and 0.919 (p < 0.001), respectively, indicating that %D of Ser may reflect protein turnover or catabolism in certain tissues as well as renal proximal tubular dysfunction.
Fukushima
T
T
Faculty of Pharmaceutical Sciences, University of Tokyo, Japan.
Santa
T
T
Homma
H
H
Nagatomo
R
R
Imai
K
K
D000596:Amino Acids / Q000097:blood*; D001806:Blood Urea Nitrogen; D002851:Chromatography, High Pressure Liquid; D003404:Creatinine / Q000097:blood; D006801:Humans; D007674:Kidney Diseases / Q000097:blood* / Q000503:physiopathology
D000596:Amino Acids; D003404:Creatinine