Gene transfer into the medfly, Ceratitis capitata, with a Drosophila hydei transposable element.
Exogenous functional DNA was introduced into the germline chromosomes of the Mediterranean fruit fly (medfly) Ceratitis capitata with a germline transformation system based on the transposable element Minos from Drosophila hydei. Transformants were identified as phenotypic revertants of a white-eyed mutation carried by the recipient strain. Clusters of transformants were detected among the progeny of 390 individuals screened for germline transformation. Five independent and phenotypically active integration events were identified, in each of which a single copy of the transposon was inserted into a different site of the medfly genome. Molecular analysis indicates that they represent transposase-mediated insertions of the transposon into medfly chromosomes.
Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology-Hellas, Crete, Greece.
T G
TG
Loukeris
I
I
Livadaras
B
B
Arcà
S
S
Zabalou
C
C
Savakis
D000818:Animals; D030801:Animals, Genetically Modified; D003433:Crosses, Genetic; D004251:DNA Transposable Elements; D004175:Diptera / Q000235:genetics*; D004330:Drosophila / Q000235:genetics*; D005127:Eye Color / Q000235:genetics; D005260:Female; D018014:Gene Transfer Techniques; D017344:Genes, Insect; D005822:Genetic Vectors; D006358:Hot Temperature; D008297:Male; D010641:Phenotype; D014170:Transformation, Genetic
D004251:DNA Transposable Elements