Influence of brodimoprim on polymorphonuclear leukocyte phagocytosis and oxidant radical production.
Antibiotics not only reach the site of infection, but also penetrate cyclically, during a treatment, into polymorphonuclear leukocytes (PMNs) and may influence their functions positively or negatively. With reference to these aspects, the influence of brodimoprim (BMP), a dimethoxybenzylpyrimidine recently entered into clinical use, on human PMN phagocytosis and oxidant radical production (chemiluminescence) was investigated. PMNs from healthy adult donors were incubated for 50 min in medium alone or in medium containing increasing concentrations (3.7, 7.5, 15, and 30 micrograms/ml) of BMP and trimethoprim (TMP). In unwashed PMNs, phagocytosis was not modified by BMP, but was significantly reduced by 30 micrograms/ml TMP; chemiluminescence was significantly reduced by 15 and 30 micrograms/ml BMP and by all concentrations of TMP. When PMNs were washed after incubation, phagocytosis was unaffected and chemiluminescence was significantly restored. BMP at therapeutic concentrations did not influence PMNs and was less toxic than TMP.
Department of Pharmacology, University of Milan, Italy.
P C
PC
Braga
M
M
Dal Sasso
S
S
Maci
L
L
Allegra
E
E
Fonti
A
A
Ghessi
S
S
Reggio
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005493:Folic Acid Antagonists / Q000494:pharmacology*; D005609:Free Radicals; D006801:Humans; D007962:Leukocytes / Q000187:drug effects*; D010587:Phagocytosis / Q000187:drug effects*; D013997:Time Factors; D014295:Trimethoprim / Q000031:analogs & derivatives* / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D005493:Folic Acid Antagonists; D005609:Free Radicals; D014295:Trimethoprim; C037079:brodimoprim