Signal transduction mechanism in response to aflatoxin B1 exposure: phosphatidylinositol metabolism.
A single dose of aflatoxin B1 (7 mg/kg body weight) to male Wistar rats significantly stimulated the hepatic activity of phosphatidylinositol kinase, a key enzyme in the cell signalling mechanism, 1-7 h following its administration. Phosphatidylinositol 4-phosphate kinase activity showed only marginal increase, whereas activities of diacylglycerol kinase and phosphatidylinositol synthetase remained unchanged. The level of diacylglycerol, however, recorded a sharp increase at 1 and 2 h after carcinogen treatment. The stimulation of phosphatidylinositol cycle with faster turnover of active second messengers might be an early step in the manifestation of toxicity and/or carcinogenicity.
Mistry
K J
KJ
Radiation Biology and Biochemistry Division, Bhabha Atomic Research Centre, Bombay, India.
Krishna
M
M
Bhattacharya
R K
RK
D019870:1-Phosphatidylinositol 4-Kinase; D016604:Aflatoxin B1 / Q000008:administration & dosage / Q000633:toxicity*; D000818:Animals; D002273:Carcinogens / Q000008:administration & dosage / Q000633:toxicity*; D019852:Diacylglycerol Kinase; D004075:Diglycerides / Q000032:analysis; D004305:Dose-Response Relationship, Drug; D007274:Injections, Intraperitoneal; D008099:Liver / Q000201:enzymology; D008297:Male; D010712:Phosphatidic Acids / Q000378:metabolism; D010716:Phosphatidylinositols / Q000378:metabolism*; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction
C011439:1,2-diacylglycerol; D002273:Carcinogens; D004075:Diglycerides; D010712:Phosphatidic Acids; D010716:Phosphatidylinositols; D016604:Aflatoxin B1; D017853:Phosphotransferases (Alcohol Group Acceptor); D019852:Diacylglycerol Kinase; D019870:1-Phosphatidylinositol 4-Kinase