Isolation and characterization of a mutation that alters the substrate specificity of the Escherichia coli glucose permease.
We isolated 10 mannitol-positive mutants from a mannitol-negative Escherichia coli strain. These mutations mapped within ptsG, encoding the glucose permease (EIIGlc), and resulted in a G-320-to-V substitution that allows EIIGlc to transport mannitol. Gly-320 lies within a putative transmembrane helix of EIIGlc that may be involved in substrate recognition.
Begley
G S
GS
Department of Biology, Boston University, Massachusetts 02215, USA.
Warner
K A
KA
Arents
J C
JC
Postma
P W
PW
Jacobson
G R
GR
D000595:Amino Acid Sequence; D002874:Chromosome Mapping; D004926:Escherichia coli / Q000201:enzymology*; D007700:Kinetics; D008353:Mannitol / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000235:genetics / Q000378:metabolism*; D010766:Phosphorylation; D013329:Structure-Activity Relationship; D013379:Substrate Specificity
D008353:Mannitol; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System; C031629:phosphoenolpyruvate-glucose phosphotransferase