Monoamine oxidase A-inhibiting components of urinary tribulin: purification and identification.
The endogenous monoamine oxidase (MAO) inhibitory activity, termed tribulin, contains several components. We have previously identified one of them, isatin, which is a selective inhibitor of MAO B. In the present study we have purified several further components of human urinary tribulin which act as selective inhibitors of MAO A. They have been identified by gas chromatography-mass spectrometry (GC-MS) as ethyl indole-3-acetate (and/or methyl indole-3-propionate), methyl indole-3-acetate and ethyl 4-hydroxyphenylacetate. IC50 values for MAO A were found to be 44 microM (105 microM for methyl indole-3-propionate), 88 microM and 120 microM, respectively, whilst those for MAO B were each greater than 1 mM. The artificial formation of these esters was excluded by carrying the parent acids, from which they are presumably synthesized, through the purification procedure. As tribulin output is increased during stress or anxiety, these results point to a possible role for tryptamine and tyramine pathways in such disorders.
Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, Moscow, Russia.
A E
AE
Medvedev
J
J
Halket
B L
BL
Goodwin
M
M
Sandler
V
V
Glover
D001792:Blood Platelets / Q000201:enzymology; D002850:Chromatography, Gel; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007211:Indoles / Q000652:urine; D007510:Isatin; D008995:Monoamine Oxidase / Q000378:metabolism; D008996:Monoamine Oxidase Inhibitors / Q000302:isolation & purification / Q000652:urine*; D010920:Placenta / Q000201:enzymology; D011963:Receptors, GABA-A / Q000187:drug effects; D012015:Reference Standards; D014363:Tryptamines / Q000652:urine; D014439:Tyramine / Q000652:urine
D007211:Indoles; D008996:Monoamine Oxidase Inhibitors; D011963:Receptors, GABA-A; D014363:Tryptamines; D007510:Isatin; C039776:tribulin; D008995:Monoamine Oxidase; D014439:Tyramine