Identification and characterization of the v-cath gene of the baculovirus, CfMNPV.
The v-cath gene of the Autographa californica multi-nucleocapsid nuclear polyhedrosis virus (AcMNPV) encodes a cathepsin L-like proteinase which plays a role in the liquefaction of host tissues during a viral infection [1]. We have identified a homologous gene in the spruce budworm virus, Choristoneura fumiferana MNPV (CfMNPV). The CfMNPV v-cath gene is 74% identical to AcMNPV v-cath at the nucleotide sequence level and 80% identical at the level of predicted amino acid sequence. Transcription analysis of the CfMNPV v-cath gene revealed that it is expressed late in infection and that transcription initiates within the consensus baculovirus late-promoter motif.
Department of Microbiology and Immunology, Queen's University, Kingston, Ontario, Canada.
J E
JE
Hill
J
J
Kuzio
P
P
Faulkner
D000595:Amino Acid Sequence; D016367:Baculoviridae / Q000235:genetics*; D001483:Base Sequence; D056668:Cathepsin L; D002403:Cathepsins / Q000235:genetics; D003546:Cysteine Endopeptidases; D010450:Endopeptidases; D005814:Genes, Viral; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D016415:Sequence Alignment
D002403:Cathepsins; D010450:Endopeptidases; D003546:Cysteine Endopeptidases; D056668:Cathepsin L