Lymphocyte proliferative responses to recombinant bovine herpes virus type 1 (BHV-1) glycoprotein gD (gIV) in immune cattle: identification of a T cell epitope.
The lymphocyte proliferative response to BHV-1 in immune cattle was compared to recombinant wild-type gD and truncated gD produced from recombinant vaccinia viruses. The response exhibited by recombinant proteins was comparable to the response induced by BHV-1 suggesting that gD is the major target structure for stimulation of bovine lymphocytes. Analysis of the proliferative response using vaccinia virus vectors expressing various modified forms of gD identified a region between residues 165 and 216 recognized by T-lymphocytes of immune cattle. Further analysis by overlapping peptides in this region localized the T cell epitope to residues 161-172. Antibody-blocking studies demonstrated that lymphocytes responding to this epitope are CD4+. In addition, lymphocytes stimulated with gD or peptide 77 (residues 161-172) also produced IFN-gamma and IL-2.
Veterinary Infectious Disease Organization, University of Saskatchewan, Saskatoon, Canada.
S K
SK
Tikoo
M
M
Campos
Y I
YI
Popowych
S
S
van Drunen Littel-van den Hurk
L A
LA
Babiuk
D000818:Animals; D002417:Cattle; D000939:Epitopes / Q000032:analysis*; D007242:Herpesvirus 1, Bovine / Q000235:genetics / Q000276:immunology*; D008213:Lymphocyte Activation; D011994:Recombinant Proteins / Q000276:immunology; D013601:T-Lymphocytes / Q000737:chemistry / Q000276:immunology*; D014759:Viral Envelope Proteins / Q000276:immunology*
D000939:Epitopes; D011994:Recombinant Proteins; D014759:Viral Envelope Proteins; C039447:glycoprotein D, Human herpesvirus 1