The immune profile of multiple sclerosis: T-lymphocyte effects predominate over all other factors in cyclophosphamide-treated patients.
It is widely believed that multiple sclerosis is a T-cell mediated autoimmune disease associated with abnormalities in immunoregulation. This large, prospective study evaluated the lymphocyte immunophenotypic profile of 246 MS patients, divided clinically into a remitting/relapsing group (n = 176) and a progressive group (n = 70), and compared their results to those of 117 healthy controls. All patients were found to have significantly elevated percentage and absolute numbers of IL2R+CD3+ cells as well as depressed percentages of CD45RA+CD4+ cells. However, when the factor of treatment with cyclophosphamide (CY) versus no treatment or treatment with other agents was used to group patients, dramatic declines in both percentages and absolute numbers of CD45RA+CD4+ cells were discovered. These declines were associated specifically with CY and and could be explained by this factor independent of the clinical state of the patient. The effects were seen in patients undergoing current treatment or in those exposed to CY in the near or remote past. These findings highlight the confounding effect of specific treatments on the immune profile of MS patients groups and suggest that there may be important implications for cellular function and clinical outcome in these and other patient groups.
Becton Dickinson Immunocytometry Systems, Erembodegem-Aalst, Belgium.
K
K
Strauss
F
F
Hulstaert
V
V
Deneys
A M
AM
Mazzon
I
I
Hannet
M
M
De Bruyère
T
T
Reichert
C J
CJ
Sindic
D000328:Adult; D000367:Age Factors; D017252:CD3 Complex / Q000378:metabolism; D018791:CD4 Lymphocyte Count; D003520:Cyclophosphamide / Q000008:administration & dosage; D018450:Disease Progression; D005260:Female; D006801:Humans; D016130:Immunophenotyping; D007165:Immunosuppression Therapy; D007376:Interleukin-2 / Q000378:metabolism; D017493:Leukocyte Common Antigens / Q000378:metabolism; D016014:Linear Models; D016131:Lymphocyte Subsets / Q000378:metabolism; D008297:Male; D009103:Multiple Sclerosis / Q000188:drug therapy / Q000276:immunology*; D011446:Prospective Studies; D012008:Recurrence; D012737:Sex Factors; D013601:T-Lymphocytes / Q000737:chemistry / Q000276:immunology*
D017252:CD3 Complex; D007376:Interleukin-2; D003520:Cyclophosphamide; D017493:Leukocyte Common Antigens