The rearranged L-myc fusion gene (RLF) encodes a Zn-15 related zinc finger protein.
We have previously characterized intrachromosomal rearrangements at 1p32 fusing the first exon of the RLF gene with L-myc. Here we present the full-length cDNA sequence of the 6251 bp RLF mRNA. The predicted 1914 amino acid Rlf protein contains sixteen widely spaced zinc finger motifs, and is related to the Zn-15 transcription factor. RLF is widely expressed in fetal and adult tissues, suggesting that it has a general role in transcriptional regulation. The zinc fingers are not contained in the 79 amino acid N-terminal region of RLF involved in the RLF-L-myc fusions, and the transforming ability of the RLF-L-myc and the normal L-myc proteins is indistinguishable. These findings suggest that the role of the rearrangements fusing RLF and L-myc is to deregulate the tightly controlled expression of the L-myc gene.
Molecular/Cancer Biology Laboratory, Haartman Institute, University of Helsinki, Finland.
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Mäkelä
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Hellsten
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Vesa
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Hirvonen
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Palotie
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Peltonen
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Alitalo
D000328:Adult; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000502:physiology; D015321:Gene Rearrangement; D016259:Genes, myc; D020662:Guanine Nucleotide Exchange Factors; D006801:Humans; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis; D015534:Trans-Activators / Q000235:genetics* / Q000502:physiology; D014157:Transcription Factors / Q000235:genetics*; D016335:Zinc Fingers
D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D020662:Guanine Nucleotide Exchange Factors; C097542:RLF protein, human; D012333:RNA, Messenger; C490805:Rgl2 protein, mouse; D015534:Trans-Activators; D014157:Transcription Factors