HSP27 phosphorylation-mediated resistance against actin fragmentation and cell death induced by oxidative stress.
Phosphorylation of heat shock protein 27 (HSP27) has been suggested to play an important role in the regulation of F-actin dynamics in response to growth factors and stress. Because the microfilament network is one of the earliest targets of oxidative stress and because phosphorylation of HSP27 is strongly induced by reactive oxygen metabolites, we have investigated the role of HSP27 phosphorylation in regulating actin dynamics in response to oxidative stress. Experiments were done in Chinese hamster CCL39 cells overexpressing various levels of the wild-type or a nonphosphorylatable form of human HSP27 (pm3 HSP27). In control cells, both H2O2 and menadione induced fragmentation of F-actin, which forms aggregates and patches concentrated around the nucleus. Stable overexpression of wild-type HSP27, but not of pm3 HSP27, conferred resistance against actin fragmentation, suggesting that HSP27 has a phosphorylation-activated protective function against actin disruption by oxidative stress. Cell lines that overexpressed the highest levels of the wild-type form of human HSP27 also showed an increased cell survival following exposure to H2O2. In contrast, cells expressing pm3 HSP27 were as sensitive as the controls to the lethal effect of H2O2. These results suggest that phosphorylation of HSP27 is causally related to the regulation of microfilament dynamics following oxidative stress and may be involved in mediating an adaptive response to oxyradical-generating agents such as carcinogens, anticancer drugs, and other xenobiotics.
Centre de Recherche en Cancérologie, Université Laval, L'Hôtel-Dieu de Québec, Canada.
J
J
Huot
F
F
Houle
D R
DR
Spitz
J
J
Landry
D000199:Actins / Q000378:metabolism*; D000818:Animals; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000378:metabolism; D016923:Cell Death / Q000187:drug effects / Q000502:physiology; D006224:Cricetinae; D003412:Cricetulus; D006360:Heat-Shock Proteins / Q000378:metabolism*; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity; D018384:Oxidative Stress / Q000502:physiology*; D010766:Phosphorylation; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology
D000199:Actins; D006360:Heat-Shock Proteins; D006861:Hydrogen Peroxide; D017871:Calcium-Calmodulin-Dependent Protein Kinases