[Opioid receptors altered binding nature in guinea-pig brain following the development of morphine dependence].
Morphine is well known to produce tolerance and dependence. The mechanisms for these phenomena are not clearly understood and there are a number of conflicting reports that chronic morphine administration decreases, increases, or leaves unchanged the number of opioid binding sites. We examined the potency of MScontin (oral controlled-release preparation of morphine) to induce morphine dependence and also determined the change of mu, delta and kappa opioid receptor types in brain homogenates obtained from morphine-dependent guinea-pigs. 1. Guinea-pigs were implanted subcutaneously with MScontin (300 mg.kg-1) and naloxone was employed to precipitate jumping behavior of withdrawal symptoms at various times. The highest degree of physical dependence was observed on the 2nd day after implantation. Therefore, this period was chosen to investigate opioid receptor binding assay. 2. Two days after implantation, the binding of 3H-DAGO (mu agonist), 3H-DPDPE (delta agonist) and 3H-U69593 (kappa agonist) to brain membranes prepared from morphine dependent and control guinea-pigs was determined. Scatchard plot of the saturation binding data revealed an increase in Bmax values (maximum specific binding) and no change in the Kd values (equilibrium dissociation constants) of 3H-opioid ligand bindings obtained from morphine-dependent animals as compared to controls. These results indicate that brain mu, delta and kappa opioid receptors are up-regulated in morphine dependent guinea-pigs.
Ohta
S
S
Department of Anesthesiology & Critical Care Medicine, Gifu University School of Medicine.
Niwa
M
M
Nozaki
M
M
Hattori
M
M
Shimonaka
H
H
Dohi
S
S
D000818:Animals; D001921:Brain / Q000378:metabolism*; D006168:Guinea Pigs; D008297:Male; D009021:Morphine Dependence / Q000378:metabolism*; D011957:Receptors, Opioid / Q000378:metabolism*; D015854:Up-Regulation
D011957:Receptors, Opioid