Simple and rapid analysis of lamotrigine, a novel antiepileptic, in human serum by high-performance liquid chromatography using a solid-phase extraction technique.
A simple and rapid method for the quantitation of concentrations of lamotrigine, a novel antiepileptic, in human serum was developed with high-performance liquid chromatography, using a solid-phase extraction technique. The mobile phase was composed of acetonitrile-10 mM phosphate buffer (pH 3.5) containing 5 mM sodium octanesulphonate (27:73, v/v), and components were detected at 265 nm. Retention times of acetanilide as an internal standard and lamotrigine were 3.4 and 10.3 min, respectively. The coefficients of variation were 3.1-4.5% and 4.4-9.8% for the within-day and between-day precision estimates, respectively. The extraction recovery of lamotrigine added to blank serum was 86-107%. The quantitation limit of lamotrigine was ca. 0.2 microgram/ml in 100 microliters of serum. These results suggest that the method employed in this study is useful for the routine monitoring of serum concentrations of lamotrigine in epileptic patients.
Department of Hospital Pharmacy, Okayama University Medical School, Japan.
S
S
Yamashita
K
K
Furuno
H
H
Kawasaki
Y
Y
Gomita
H
H
Yoshinaga
Y
Y
Yamatogi
S
S
Ohtahara
D000927:Anticonvulsants / Q000097:blood* / Q000493:pharmacokinetics; D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007202:Indicators and Reagents; D000077213:Lamotrigine; D013056:Spectrophotometry, Ultraviolet; D014227:Triazines / Q000097:blood* / Q000493:pharmacokinetics
D000927:Anticonvulsants; D007202:Indicators and Reagents; D014227:Triazines; D000077213:Lamotrigine