RNA expression and chromosomal location of the mouse long-chain acyl-CoA dehydrogenase gene.
The cDNA for mouse long-chain acyl-CoA dehydrogenase (Acadl, gene symbol; LCAD, enzyme) was cloned and characterized. The cDNA was obtained by library screening and reverse transcription-polymerase chain reaction (RT-PCR). The deduced amino acid sequence showed a high degree of homology to both the rat and the human LCAD sequence. Northern analysis of multiple tissues using the mouse Acadl cDNA as a probe showed two bands in all tissues examined. We found a total of three distinct mRNAs for Acadl. These three mRNAs were encoded by a single gene that we mapped to mouse chromosome 1. The three transcripts differed in the 3' untranslated region due to use of alternative polyadenylation sites. Quantitative evaluation of a multitissue Northern blot showed a varied ratio of the larger transcript as compared with the smaller transcripts.
Department of Comparative Medicine, School of Medicine, University of Alabama at Birmingham 35294-0019, USA.
M E
ME
Hinsdale
S C
SC
Farmer
K R
KR
Johnson
M T
MT
Davisson
D A
DA
Hamm
R J
RJ
Tolwani
P A
PA
Wood
D044942:Acyl-CoA Dehydrogenase, Long-Chain / Q000096:biosynthesis / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D003433:Crosses, Genetic; D018076:DNA, Complementary / Q000235:genetics; D004790:Enzyme Induction; D005260:Female; D005796:Genes; D006801:Humans; D008297:Male; D051379:Mice / Q000235:genetics*; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D005810:Multigene Family; D009115:Muridae / Q000235:genetics; D009928:Organ Specificity; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics; D051381:Rats; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D014158:Transcription, Genetic
D018076:DNA, Complementary; D012333:RNA, Messenger; D044942:Acyl-CoA Dehydrogenase, Long-Chain