Electroporation-induced damage in mammalian cell DNA.
Electroporation induced damage in the DNA of HL60 cells has been investigated by alkaline elution techniques. DNA damage is minimised by reducing the total charge applied (i.e., voltage x capacitance). Reduction of either of these electrical parameters, however, compromises the induced permeability of the cells to small molecules. The data presented concerning the effects of voltage and capacitance on DNA damage and the permeability of cells can be used to specify optimum conditions for electroporation in which DNA damage is minimised. The duration for which the current is applied can be seen to have a significant effect on the level of DNA damage. A modest temperature rise may occur when an electric charge is passed through electroporation buffer, but this event alone does not induce DNA damage in cells. The effect of voltage upon the permeability of HL60 cells to fluorescent-labelled molecules of varying molecular weight is reported.
Meaking
W S
WS
School of Biochemistry, University of Birmingham, Edgbaston, UK.
Edgerton
J
J
Wharton
C W
CW
Meldrum
R A
RA
D002021:Buffers; D002470:Cell Survival; D004247:DNA / Q000096:biosynthesis*; D004249:DNA Damage; D004574:Electromagnetic Fields; D018274:Electroporation; D006801:Humans; D013696:Temperature; D014162:Transfection; D014407:Tumor Cells, Cultured
D002021:Buffers; D004247:DNA