Collisionally-activated dissociation spectra of linear peptides in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.
Collisionally-activated dissociation (CAD) studies of linear peptides were performed using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Four different gases, viz. helium, nitrogen, argon and xenon, were introduced into a gas cell, installed close to the ion source of TOF mass spectrometer, to obtain high-energy CAD information. The CAD spectral patterns for the linear peptides obtained using the four gases were almost the same as each other. The fragments characteristically obtained in conventional high-energy CAD using tandem mass spectrometry were observed also in these spectra. A difference between CAD spectra and post-source decay product-ion spectra has been demonstrated.
Analytical and Metabolic Research Laboratories, Sankyo Co. Ltd., Tokyo, Japan.
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Kosaka
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Ishikawa
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Kinoshita
D000595:Amino Acid Sequence; D008969:Molecular Sequence Data; D010455:Peptides / Q000032:analysis*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D010455:Peptides