Genetic polymorphism of the H1 subunit of inter-alpha-trypsin inhibitor.
Inter-alpha-trypsin inhibitor (ITI) consists of 3 subunits (H1, H2 and L) which are encoded by 3 distinct genes. We attempted to prepare the subunit-specific monoclonal antibodies to identify the subunit carrying ITI polymorphism. Three monoclonal antibodies which were specific against each of these 3 subunits (ITI H1, ITI H2 and ITI L) were selected. ITI types could be detected by anti ITI H1 antibody, but not by anti ITI H2 or anti ITI L antibodies. From the result, it was proved that ITI polymorphism originates from the ITI H1 gene products, therefore we propose to call this system ITI H1 polymorphism.
Harada
A
A
Department of Forensic Medicine, Yamagata University School of Medicine, Japan.
Umetsu
K
K
Suzuki
T
T
D000510:Alpha-Globulins / Q000235:genetics*; D000818:Animals; D000911:Antibodies, Monoclonal; D015153:Blotting, Western; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D010641:Phenotype; D011110:Polymorphism, Genetic
D000510:Alpha-Globulins; D000911:Antibodies, Monoclonal; C011877:inter-alpha-inhibitor