Crystallization and preliminary X-ray crystallographic analysis of arylesterase from Vibrio mimicus.
Single crystals of arylesterase (EC 3.1.1.2) from Vibrio mimicus have been obtained from ammonium sulfate as a precipitant at room temperature for 2 months. The present crystals diffract up to 2.2 A resolution and belong to monoclinic space group P2(1). The cell dimensions are a = 55.65(1) A, b = 53.46(1) A, c = 65.79(1) A, and beta = 106.54(1) degrees. There are two molecules of molecular weight 22 kDa in an asymmetric unit with a solvent content of 43%.
Musayev
F N
FN
Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan, Republic of China.
Lee
Y L
YL
Shaw
J F
JF
Liaw
Y C
YC
D000645:Ammonium Sulfate; D002265:Carboxylic Ester Hydrolases / Q000737:chemistry*; D011232:Chemical Precipitation; D003460:Crystallization; D018360:Crystallography, X-Ray; D006863:Hydrogen-Ion Concentration; D014733:Vibrio / Q000201:enzymology*
D002265:Carboxylic Ester Hydrolases; C019734:arylesterase; D000645:Ammonium Sulfate