An anti-tumor necrosis factor antibody suppresses sleep in rats and rabbits.
It is hypothesized that tumor necrosis factor (TNF) is an endogenous sleep-promoting substance. In the present experiments we studied the effects of a monoclonal anti-TNF antibody in rats and rabbits. Seven rats and 14 rabbits were implanted with electroencephalographic electrodes, a brain thermistor and an intracerebroventricular guide cannula. The animals were injected with saline, control IgG, and monoclonal hamster anti-murine-TNF antibodies (TNFab) on 3 separate days. Ten micrograms TNFab suppressed non-rapid-eye-movement sleep (NREMS) in rats. In rabbits, 2.5 micrograms TNFab did not affect sleep but decreased brain temperature; in contrast, 25 micrograms TNFab suppressed NREMS without affecting brain temperature. These results are consistent with the hypothesis that endogenous TNF plays an important role in sleep regulation.
Takahashi
S
S
Department of Physiology and Biophysics, University of Tennessee, Memphis 38163, USA.
Kapás
L
L
Fang
J
J
Krueger
J M
JM
D000704:Analysis of Variance; D000818:Animals; D000911:Antibodies, Monoclonal; D001833:Body Temperature Regulation / Q000502:physiology; D002552:Cerebral Ventricles; D006224:Cricetinae; D004569:Electroencephalography; D008297:Male; D011817:Rabbits; D051381:Rats; D017207:Rats, Sprague-Dawley; D012890:Sleep / Q000502:physiology*; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology*
D000911:Antibodies, Monoclonal; D014409:Tumor Necrosis Factor-alpha