Enhanced tumor specificity of 741F8-1 (sFv')2, an anti-c-erbB-2 single-chain Fv dimer, mediated by stable radioiodine conjugation.
The goal of this study was to determine if the stabilization of the radioiodine-protein bond by the N-succinimidyl p-iodobenzoate (PIB) method improved the degree and specificity of tumor localization of 125I-741F8-1 (sFv')2, an anti-c-erbB-2 sFv dimer, in an immunodeficient murine model.


METHODS
Gamma camera images were acquired 21 hr after intravenous administration of 131I-741F8-1 (sFv')2 labeled by the p-iodobenzoate or chloramine T methods. The stability of the radioiodine-protein bond also was assessed in plasma samples after intravenous injection of 125I-741F8-1 (sFv')2 labeled by either the chloramine T or p-iodobenzoate methods.


RESULTS
By 6 hr postinjection, 97% of the activity associated with the 125I-741F8-1 (sFv')2 labeled by the p-iodobenzoate method was protein bound compared with 61% after labeling with the chloramine-T method. These observations indicate that increasing the stability of the conjugation between the radioiodine and the sFv molecule can significantly increase the degree and specificity of tumor targeting. Significantly greater tumor retention (p < 0.005) and lower blood (p < 0.001), spleen (p < 0.001) and stomach (p < 0.005) retention were observed in biodistribution studies when the p-iodobenzoate conjugate was used. This resulted in superior tumor-to-organ ratios for all tissue samples studied.


CONCLUSIONS
These observations may have clinical relevance for the use of radiolabeled sFv as imaging agents.
Department of Medical Oncology, Fox Chase Cancer Center, Philadelphia, Pennsylvania.
G P
GP
Adams
J E
JE
McCartney
E J
EJ
Wolf
J
J
Eisenberg
J S
JS
Huston
M A
MA
Bookman
P
P
Moldofsky
W F
WF
Stafford
L L
LL
Houston
L M
LM
Weiner
D000818:Animals; D000911:Antibodies, Monoclonal; D007457:Iodine Radioisotopes; D007553:Isotope Labeling; D051379:Mice; D016513:Mice, SCID; D009374:Neoplasms, Experimental / Q000000981:diagnostic imaging*; D016719:Radioimmunodetection; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000276:immunology; D014018:Tissue Distribution
D000911:Antibodies, Monoclonal; D007457:Iodine Radioisotopes; D018719:Erb-b2 Receptor Tyrosine Kinases