Analysis of saturation transfer electron paramagnetic resonance spectra of a spin-labeled integral membrane protein, band 3, in terms of the uniaxial rotational diffusion model.
Algorithms have been developed for the calculation of saturation transfer electron paramagnetic resonance (ST-EPR) spectra of a nitroxide spin-label assuming uniaxial rotational diffusion, a model that is frequently used to describe the global rotational dynamics of large integral membrane proteins. One algorithm explicitly includes terms describing Zeeman overmodulation effects, whereas the second more rapid algorithm treats these effects approximately using modified electron spin-lattice and spin-spin relaxation times. Simulations are presented to demonstrate the sensitivity of X-band ST-EPR spectra to the rate of uniaxial rotational diffusion and the orientation of the nitroxide probe with respect to the diffusion axis. Results obtained by using the algorithms presented, which are based on the transition-rate formalism, are in close agreement with those obtained by using an eigenfunction expansion approach. The effects of various approximations used in the simulation algorithms are considered in detail. Optimizing the transition-rate formalism to model uniaxial rotational diffusion results in over an order of magnitude reduction in computation time while allowing treatment of nonaxial A- and g-tensors. The algorithms presented here are used to perform nonlinear least-squares analyses of ST-EPR spectra of the anion exchange protein of the human erythrocyte membrane, band 3, which has been affinity spin-labeled with a recently developed dihydrostilbene disulfonate derivative, [15N,2H13]-SL-H2DADS-MAL. These results suggest that all copies of band 3 present in intact erythrocytes undergo rotational diffusion about the membrane normal axis at a rate consistent with a band 3 dimer.
Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee, USA.
E J
EJ
Hustedt
A H
AH
Beth
D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid / Q000031:analogs & derivatives; D000465:Algorithms; D001457:Anion Exchange Protein 1, Erythrocyte / Q000737:chemistry*; D004058:Diffusion; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods; D004910:Erythrocyte Membrane / Q000502:physiology; D006801:Humans; D008962:Models, Theoretical; D012399:Rotation; D013113:Spin Labels
C097423:4'-maleimido-4-((1-oxyl-2,2,5,5-tetramethyl-3-maleimidyl)carbonylamino)dihydrostilbene-2,2'-disulfonate; D001457:Anion Exchange Protein 1, Erythrocyte; D013113:Spin Labels; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid