Purification and some properties of S-Hemolysin produced by Streptomyces sp. strain no. A-6288.
A new cytolytic toxin, designated as S-Hemolysin, was found in the culture filtrate of Streptomyces sp. strain No. A-6288, isolated from a soil sample. The molecular weight of S-Hemolysin was estimated to be 10,000 by SDS-polyacrylamide gel electrophoresis and to be 20,000 by Sephadex G-100. S-Hemolysin is a glycoprotein that is composed of 102 amino acid residues with 11.6% glucose, and the isoelectric point is around pH 5.8. The phospholipase C activity of S-Hemolysin was specific for the following substrates in this order: sphingomyelin > lysophosphatidylethanolamine > lysophosphatidylcholine > phosphatidylethanolamine > phosphatidylcholine. S-Hemolysin had hemolytic activity against rabbit, human, and sheep erythrocytes, but did not cause aggregation of human platelets. These activities were accelerated with Mg2+, Mn2+, and Co2+ ions and inhibited by the addition of Ca2+, Cu2+, and Zn2+ ions. This enzyme was shown to be different from the known bacterial phospholipase C.
Laboratory of Medicinal Microbiology, Faculty of Pharmaceutical Sciences, Kumamoto University, Japan.
K
K
Suzuki
K
K
Matsunaga
T
T
Ehara
Y
Y
Sakumura
T
T
Siddique
M
M
Uyeda
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001792:Blood Platelets / Q000187:drug effects; D002241:Carbohydrates / Q000032:analysis; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000187:drug effects; D006460:Hemolysin Proteins / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology; D006801:Humans; D007526:Isoelectric Point; D008670:Metals / Q000494:pharmacology; D008970:Molecular Weight; D010743:Phospholipids / Q000378:metabolism; D011817:Rabbits; D012756:Sheep; D013302:Streptomyces / Q000378:metabolism*; D010738:Type C Phospholipases / Q000378:metabolism
D000596:Amino Acids; D002241:Carbohydrates; D006460:Hemolysin Proteins; D008670:Metals; D010743:Phospholipids; D010738:Type C Phospholipases