An improved GLC method for a rapid, simultaneous analysis of both medium chain fatty acids and medium chain triglycerides in plasma.
An improved gas-liquid chromatographic (GLC) method for the direct, simultaneous analysis of both medium chain monocarboxylic acids (MCFA) and medium chain triglycerides (MCT) is reported. The calibration curve of MCFA and MCT is linear in the range from 30 ng to 1000 ng. Five nanograms for tricaproin (MCT-6), 10 ng for tricaprylin (MCT-8) and 15 ng for tricaprin (MCT-9) represent the GLC detection limits of MCT, while those of MCFA range from 40 to 15 ng depending on their chain length: the longer the chain length, the higher the detection limit. The recovery of MCFA range from 79 to 99% and that of MCT from 85 to 99%. An example of plasma concentration curves of MCT and MCFA after an intravenous bolus injection of an MCT emulsion (100 mg MCT/kgbw) in a patient with Crohn's disease is shown.
Mingrone
G
G
Istituto di Clinica Medica, Universita Cattolica del S. Cuore, Rome, Italy.
Greco
A V
AV
Capristo
E
E
Benedetti
G
G
Castagneto
M
M
Gasbarrini
G
G
D000328:Adult; D002138:Calibration; D002210:Caprylates / Q000008:administration & dosage / Q000493:pharmacokinetics; D002849:Chromatography, Gas; D003424:Crohn Disease / Q000097:blood / Q000378:metabolism; D003652:Decanoic Acids / Q000008:administration & dosage / Q000493:pharmacokinetics; D005227:Fatty Acids / Q000097:blood* / Q000493:pharmacokinetics; D006801:Humans; D007202:Indicators and Reagents; D007275:Injections, Intravenous; D007700:Kinetics; D008297:Male; D012044:Regression Analysis; D014280:Triglycerides / Q000097:blood* / Q000493:pharmacokinetics
D002210:Caprylates; D003652:Decanoic Acids; D005227:Fatty Acids; D007202:Indicators and Reagents; D014280:Triglycerides