Fibroblasts transformed by combinations of ras, myc and mutant p53 exhibit increased phosphorylation of histone H1 that is independent of metastatic potential.
H1 histones play an important role in regulating higher order structure of chromatin and are potential regulators of gene expression. H1s are phosphorylated, a modification which alters their interaction with DNA. We measured the abundance of three phosphorylated H1 subtypes in mouse fibroblasts transformed by combinations of ras, myc and mutant p53 which differ in metastatic potential. We found that there is an increase in phosphorylation of H1 subtypes in fibroblasts transformed with ras, myc and mutant p53. This increase was found to correlate with cellular transformation but not with induction of the metastatic phenotype.
Taylor
W R
WR
Manitoba Institute of Cell Biology, University of Manitoba, Winnipeg, Canada.
Chadee
D N
DN
Allis
C D
CD
Wright
J A
JA
Davie
J R
JR
D000818:Animals; D002453:Cell Cycle; D002461:Cell Line, Transformed; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D005347:Fibroblasts / Q000378:metabolism*; D016259:Genes, myc; D016158:Genes, p53; D011905:Genes, ras; D006657:Histones / Q000378:metabolism*; D051379:Mice; D009154:Mutation; D010641:Phenotype; D010766:Phosphorylation
D006657:Histones