Oestradiol acutely stimulates exocytosis of oxytocin and vasopressin from dendrites and somata of hypothalamic magnocellular neurons.
Oestrogen has many direct or indirect actions upon the magnocellular system of the hypothalamus. We have examined the possibility of acute actions of oestrogen upon the magnocellular system by stimulating slices of supraoptic nucleus in vitro with various concentrations of oestrogen, for varying lengths of time, and assessing the intrahypothalamic release of oxytocin and vasopressin under these conditions. Slices were stimulated in the presence of tannic acid, which precipitates extracellular protein and thereby stabilizes exocytosed neurosecretory granule cores. Stimulation for 5 or 20 min of slices of hypothalamus containing the supraoptic nuclei with 2.66 nM-26.6 microM 17 beta-oestradiol benzoate caused the exocytosis of granules from both dendrites and cell bodies of the magnocellular neurons; exocytosis from the dendrites predominated. Granules of both oxytocin- and vasopressin-producing cells were exocytosed to a similar extent. The incidence of exocytosis of both hormones after stimulation by oestrogen was significantly higher than after exposure either to physiological saline or to 17 alpha-oestradiol, but significantly lower than after stimulation by 56 mM potassium. The various doses of steroid and durations of stimulation all resulted in similar amounts of captured exocytosis. Furthermore, the oestradiol-induced release was not inhibited by removal of extracellular calcium, whereas the potassium-stimulated release was abolished. Exposure for 20 min to either testosterone or progesterone did not induce intranuclear release of significant numbers of neurosecretory granules from the magnocellular neurons. In contrast to its effect on the hypothalamus, 26.6 microM oestradiol for either 20 min or 5 min did not induce exocytosis of neurosecretory granules from the posterior pituitary. We conclude that oestrogen can exert acute non-genomic actions on the magnocellular neurons to promote intrahypothalamic release of oxytocin and vasopressin. This effect is probably direct on the magnocellular neurons as it is not dependent on external calcium. Such actions may be important in the development of the functional and morphological plasticity of the magnocellular system that occurs in parturition and lactation.
Department of Human Anatomy, University of Oxford, UK.
H
H
Wang
A R
AR
Ward
J F
JF
Morris
D000818:Animals; D002118:Calcium / Q000378:metabolism; D003470:Culture Media; D003712:Dendrites / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D004958:Estradiol / Q000494:pharmacology*; D005089:Exocytosis / Q000187:drug effects*; D005260:Female; D007031:Hypothalamus / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D009474:Neurons / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D010121:Oxytocin / Q000378:metabolism*; D010902:Pituitary Gland / Q000378:metabolism; D051381:Rats; D013268:Stimulation, Chemical; D013495:Supraoptic Nucleus / Q000166:cytology / Q000502:physiology; D014667:Vasopressins / Q000378:metabolism*
D003470:Culture Media; D014667:Vasopressins; D004958:Estradiol; D010121:Oxytocin; D002118:Calcium