Effect of inhibitors and substrates on methyl mercury uptake by rat erythrocytes.
Methyl mercury (MeHg) uptake by isolated erythrocytes from rats was studied at 20 degrees C. Inhibitors and substrates were used to test which transport system was involved in MeHg uptake. Ouabain and ATP were used to test the active transport system. Glycine was used to test system Gly. DL-Methionine was used to test system L. Cysteine was used to test the cysteine-facilitated transport system. The effects of Ca2+, Mg2+ and Na+ on MeHg uptake have been examined. MeHgCl and 4,4'-diisothiocyano-2,2'-stilbenedisulfonic acid (DIDS) were used to test C1- ion transport system. D-Glucose and cytochalasin B were used to test the facilitated diffusive D-Glucose transport system. Colchicine and vinblastine were used to test the microtubule system. Probenecid was used to test the organic acid transport system. Valinomycin was used to test the effect of the membrane potential on MeHg uptake. The results showed that MeHg uptake at 20 degrees C might be involved in the following transport systems: 1) an active transport system; 2) a cysteine-facilitated transport system; 3) a C1- ion transport system; 4) a facilitated diffusive D-glucose transport system; 5) an organic acid transport system. The transport systems for MeHg uptake were sensitive to the membrane potential.
Biochemistry Section, National Institute for Minamata Disease, Kumamoto, Japan.
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Wu
D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000494:pharmacology; D000596:Amino Acids / Q000494:pharmacology; D000818:Animals; D001667:Binding, Competitive; D001693:Biological Transport, Active / Q000187:drug effects; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism*; D005947:Glucose / Q000494:pharmacology; D008297:Male; D008767:Methylmercury Compounds / Q000037:antagonists & inhibitors / Q000493:pharmacokinetics*; D010042:Ouabain / Q000494:pharmacology; D011339:Probenecid / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar
D000596:Amino Acids; D008767:Methylmercury Compounds; D010042:Ouabain; D000255:Adenosine Triphosphate; D005947:Glucose; D011339:Probenecid; D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid