A hydrophobic region of ricin A chain which may have a role in membrane translocation can function as an efficient noncleaved signal peptide.
Ricin A chain is a polypeptide of 267 amino acids containing a hydrophobic region near its carboxyl-terminus (residues 245-256) which has been implicated in the membrane translocation step necessary for this catalytically active toxin to reach its intracellular substrate. DNA fusions were constructed that encoded hybrid proteins consisting of carboxyl-terminal residues 233-267 or residues 238-267 of ricin A chain preceding mouse dihydrofolate reductase. When in vitro transcripts prepared from these constructs were translated in cell-free systems, the ricin A chain-derived sequences functioned as efficient signal peptides which directed dihydrofolate reductase into microsomes or into proteoliposomes containing microsomal membrane components.
Department of Biological Sciences, University of Warwick, Coventry, UK.
J A
JA
Chaddock
L M
LM
Roberts
B
B
Jungnickel
J M
JM
Lord
D000595:Amino Acid Sequence; D000818:Animals; D001693:Biological Transport, Active; D002474:Cell-Free System; D066298:In Vitro Techniques; D051379:Mice; D008861:Microsomes / Q000378:metabolism; D008969:Molecular Sequence Data; D015394:Molecular Structure; D014176:Protein Biosynthesis; D021382:Protein Sorting Signals / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D011510:Proteolipids / Q000378:metabolism; D011817:Rabbits; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012276:Ricin / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D013762:Tetrahydrofolate Dehydrogenase / Q000235:genetics / Q000378:metabolism
D021382:Protein Sorting Signals; D011510:Proteolipids; D011993:Recombinant Fusion Proteins; C020485:proteoliposomes; D012276:Ricin; D013762:Tetrahydrofolate Dehydrogenase