Cloning and nucleotide sequence of the inulin fructotransferase (DFA I-producing) gene of Arthrobacter globiformis S14-3.
A gene encoding an inulin fructotransferase (DFA I-producing) [EC 2.4.1.200] from Arthrobacter globiformis S14-3 was cloned and the nucleotides sequenced, for the first time. The sequence indicated that the native enzyme protein is composed of 392 amino acid residues. The native enzyme is an extracellar enzyme produced in the culture supernatant of A. globiformis S14-3, but the nucleotide sequence of the gene lacks a sequence for signal peptide for secretion. The 1.5-kb DNA fragment encoding the gene was found to produce the active enzyme in the culture supernatant of an E. coli clone, under the control of the lac promoter of pUC119.
National Food Research Institute, Ministry of Agriculture, Forestry, and Fisheries, Ibaraki, Japan.
K
K
Haraguchi
K
K
Seki
M
M
Kishimoto
T
T
Nagata
T
T
Kasumi
K
K
Kainuma
S
S
Kobayashi
D000595:Amino Acid Sequence; D001173:Arthrobacter / Q000201:enzymology* / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D015342:DNA Probes; D004269:DNA, Bacterial; D004926:Escherichia coli; D005798:Genes, Bacterial; D006602:Hexosyltransferases / Q000235:genetics* / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction
D001426:Bacterial Proteins; D015342:DNA Probes; D004269:DNA, Bacterial; D006602:Hexosyltransferases; C097478:inulin fructotransferase (DFA I-producing)