The structure of the acidic exopolysaccharide produced by Pseudomonas "gingeri" strain Pf9.
The structure of the acidic exopolysaccharide produced by the mushroom pathogen Pseudomonas "gingeri" strain Pf9, a bacterium which causes ginger blotch, was investigated by chemical analysis, mass spectrometry and 1D and 2D NMR spectroscopy. The polysaccharide consists of the linear trisaccharide repeating unit [formula: see text] where the cyclic pyruvic acetal groups at O-4 and O-6 of the mannopyranosyl residues have the S-configuration. Methylation analysis under neutral conditions and NMR data showed that the mannose residues are acetylated at O-2. This exopolysaccharide has the same structure as the E. coli K55 capsular polysaccharide and differs from the Klebsiella K5 capsular polysaccharide only in the position of acetylation (C-2 of the glucopyranose residue).
US Department of Agriculture, Eastern Regional Research Center, Philadelphia, PA 19118, USA.
P
P
Cescutti
S F
SF
Osman
W F
WF
Fett
D
D
Weisleder
D001487:Basidiomycota; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008745:Methylation; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009005:Monosaccharides / Q000032:analysis; D011135:Polysaccharides, Bacterial / Q000737:chemistry*; D011549:Pseudomonas / Q000737:chemistry*
D009005:Monosaccharides; D011135:Polysaccharides, Bacterial; C064841:exopolysaccharide, Pseudomonas