Primary structure and functional expression of a cGMP-gated potassium channel.
Cyclic nucleotides modulate potassium (K) channel activity in many cells and are thought to act indirectly by inducing channel protein phosphorylation. Herein we report the isolation from rabbit of a gene encoding a K channel (Kcn1) that is specifically activated by cGMP and not by cAMP. Analysis of the deduced amino acid sequence (725 amino acids) indicates that, in addition to a core region that is highly homologous to Shaker K channels, Kcn1 also contains a cysteine-rich region similar to that of ligand-gated ion channels and a cyclic nucleotide-binding region. Northern blot analysis detects gene expression in kidney, aorta, and brain. Kcn1 represents a class of K channels that may be specifically regulated by cGMP and could play an important role in mediating the effects of substances, such as nitric oxide, that increase intracellular cGMP.
Yao
X
X
Department of Medicine, Yale University School of Medicine, West Haven Veterans Affairs Medical Center, New Haven, CT 06510, USA.
Segal
A S
AS
Welling
P
P
Zhang
X
X
McNicholas
C M
CM
Engel
D
D
Boulpaep
E L
EL
Desir
G V
GV
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D006152:Cyclic GMP / Q000378:metabolism*; D004553:Electric Conductivity; D015723:Gene Library; D015640:Ion Channel Gating; D008969:Molecular Sequence Data; D009865:Oocytes / Q000378:metabolism; D010802:Phylogeny; D011188:Potassium / Q000378:metabolism*; D015221:Potassium Channels / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D014176:Protein Biosynthesis; D011487:Protein Conformation; D012333:RNA, Messenger / Q000378:metabolism; D011817:Rabbits; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution; D014981:Xenopus
C097183:Kcn1 protein, Oryctolagus cuniculus; D015221:Potassium Channels; D012333:RNA, Messenger; D006152:Cyclic GMP; D011188:Potassium