Muscle regeneration and mitochondrial calmitine increase in the dystrophic dy/dy mouse after intramuscular chlorpromazine injection.
We studied the effect of chlorpromazine injection on the gastrocnemius muscles of C57BL/6J dy/dy dystrophic mice. Changes in mitochondrial calmitine concentrations and differences in microscopy studies, fibre typing and morphometry were compared in gastrocnemius muscles of dystrophic and control mice before and 2 and 21 days after injection. In both cases, calmitine reduction associated with muscle degeneration was observed 2 days after drug injection. Calmitine then increased, reaching a level at day 21 nearly identical to that of controls before injection. This increase was associated with muscle regeneration. These results clearly indicate that dystrophic mouse muscle can regenerate calmitine after drug-induced damage.
Groupe de Recherche sur les Myopathies, U.E.R. de Médecine, Nantes, France.
B
B
Lucas-Heron
J P
JP
Louboutin
B
B
Ollivier
N
N
Schmitt
D000818:Animals; D002135:Calcium-Binding Proteins / Q000378:metabolism*; D002155:Calsequestrin; D048429:Cell Size; D002746:Chlorpromazine / Q000494:pharmacology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008928:Mitochondria / Q000187:drug effects*; D018482:Muscle, Skeletal / Q000187:drug effects* / Q000648:ultrastructure*; D009137:Muscular Dystrophy, Animal; D013997:Time Factors
D002135:Calcium-Binding Proteins; D002155:Calsequestrin; C503130:Casq1 protein, mouse; D002746:Chlorpromazine