Diversity in lipid A binding ligands: comparison of lipid A monoclonal antibodies with rBPI23.
1. Mabs with a high affinity for free lipid A do not bind when it is covalently linked, i.e. in the form of LPS. 2. Lipid A-binding Mabs may be divided into three categories: I. Monoreactive Mabs that bind to the hydrophillic backbone of lipid A II. Polyreactive Kdo Mabs III. Polyreactive Mabs that bind by hydrophobic interactions 3. rBPI23 binds either free or covalently linked lipid A.
Appelmelk
B J
BJ
Free University, Department of Medical Microbiology, Amsterdam, The Netherlands.
Qing
A Y
AY
Helmerhorst
E J
EJ
Maaskant
J J
JJ
Abraham
P R
PR
Thijs
L G
LG
MacLaren
D M
DM
D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism*; D023181:Antimicrobial Cationic Peptides; D001665:Binding Sites; D001798:Blood Proteins / Q000378:metabolism*; D016905:Gram-Negative Bacterial Infections / Q000628:therapy; D006801:Humans; D066298:In Vitro Techniques; D008024:Ligands; D008050:Lipid A / Q000276:immunology* / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000037:antagonists & inhibitors / Q000378:metabolism / Q000633:toxicity; D008565:Membrane Proteins; D011485:Protein Binding; D017510:Protein Folding; D011994:Recombinant Proteins / Q000378:metabolism; D012772:Shock, Septic / Q000628:therapy
D000911:Antibodies, Monoclonal; D023181:Antimicrobial Cationic Peptides; D001798:Blood Proteins; D008024:Ligands; D008050:Lipid A; D008070:Lipopolysaccharides; D008565:Membrane Proteins; D011994:Recombinant Proteins; C021623:bactericidal permeability increasing protein