Clavulanic acid biosynthesis in Streptomyces clavuligerus: gene cloning and characterization.
Seven classes of Streptomyces clavuligerus mutants defective in clavulanic acid (CLA) biosynthesis have been identified and used to clone the chromosomal DNA encoding eight CLA biosynthetic genes. The complete sequences of three and the partial sequences of two of these biosynthetic genes are reported, together with their known or predicted functions.
SmithKline Beecham Pharmaceuticals, Betchworth, Surrey, UK.
J E
JE
Hodgson
A P
AP
Fosberry
N S
NS
Rawlinson
H N
HN
Ross
R J
RJ
Neal
J C
JC
Arnell
A J
AJ
Earl
E J
EJ
Lawlor
D000123:Acetyltransferases / Q000235:genetics; D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D001483:Base Sequence; D019818:Clavulanic Acid; D002969:Clavulanic Acids / Q000096:biosynthesis*; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D015183:Restriction Mapping; D013291:Streptococcus / Q000235:genetics*; D014512:Ureohydrolases / Q000235:genetics
D000900:Anti-Bacterial Agents; D002969:Clavulanic Acids; D004269:DNA, Bacterial; D019818:Clavulanic Acid; D000123:Acetyltransferases; C020646:glutamate acetyltransferase; D014512:Ureohydrolases; C094419:proclavaminate amidino hydrolase