The effect of levonorgestrel-releasing intrauterine device (20 micrograms/day) (LNG-IUD-20) on the morphological structure of human endometrium: a study of the endometrial factor VIII activity in the women before and after insertion of LNG-IUD-20 by the digital image analysis.
The specimens of endometria were obtained from 18 women using an intrauterine device releasing levonorgestrel at 20 micrograms/day (LNG-IUD-20). An immunoperoxidase reaction, PAP method, with the antiserum of Factor VIII as the primary antibody, was carried out in the endometrial biopsies to detect the Factor VIII activity in the endometrial endothelium before and after insertion of LNG-IUD-20. The immunoperoxidase activity was quantitatively assessed by a computer digital image analyser. The results revealed that there were a lower Factor VIII activity in the endometrial endothelial cells after insertion of LNG-IUD-20 (p < 0.001) when compared with the control. From the results of the present study, it is suggested that the synthesis and release of endometrial endothelial Factor VIII might be inhibited by the insertion of LNG-IUD20.
National Research Institute for Family Planning, Beijing, China.
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P
Zhu
X
X
Liu
H
H
Luo
Z
Z
Gu
J
J
Wang
L
L
Xu
J
J
Cheng
R
R
Xu
S
S
Lian
Y
Y
Cui
D000328:Adult; D003271:Contraceptive Agents, Female / Q000494:pharmacology*; D004717:Endometrium / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D005169:Factor VIII / Q000032:analysis / Q000378:metabolism*; D005260:Female; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry / Q000379:methods; D007436:Intrauterine Devices, Medicated / Q000592:standards*; D016912:Levonorgestrel / Q000494:pharmacology*; D008597:Menstrual Cycle / Q000187:drug effects / Q000502:physiology
D003271:Contraceptive Agents, Female; D016912:Levonorgestrel; D005169:Factor VIII