Efficiency of the flotation method in the isolation of Penicillium marneffei from seeded soil.
The efficiency of a modified flotation method for the isolation of Penicillium marneffei from soils has been evaluated. About 80% was recovered from sterilized soil freshly seeded with P. marneffei (100 conidia/1.5 g soil). When seeded non-sterile soil was used (at least 100 seeded conidia/1.5 g soil), P. marneffei could be effectively recovered by employing a combination of the flotation method and the mouse inoculation method.
Department of Microbiology, Faculty of Medicine, Chiang Mai University, Thailand.
N
N
Vanittanakom
M
M
Mekaprateep
P
P
Sriburee
P
P
Vanittanakom
P
P
Khanjanasthiti
D017088:AIDS-Related Opportunistic Infections / Q000382:microbiology; D000818:Animals; D006801:Humans; D008099:Liver / Q000382:microbiology; D051379:Mice / Q000382:microbiology; D009181:Mycoses / Q000209:etiology / Q000382:microbiology; D010407:Penicillium / Q000302:isolation & purification*; D012988:Soil Microbiology; D013154:Spleen / Q000382:microbiology