Molecular modeling of the GM-CSF and IL-3 receptor complexes.
A model for the structure of the cytokine interleukin-3 (IL-3) is presented based on the structural homology of the hematopoietic cytokines and utilizing the crystal structures of interleukin-5 and granulocyte macrophage colony stimulating factor (GM-CSF). In addition, models of the receptor complexes of GM-CSF and IL-3 are presented based on the structural homology of the hematopoietic receptors to growth hormone. Several key interactions between the ligands and their receptors are discovered, some in agreement with previous mutagenesis studies and others that have not yet been the subject of mutagenesis studies. The models provide insights into the binding of GM-CSF and IL-3 to their receptors.
Oxford Centre for Molecular Science, Physical and Theoretical Chemistry Laboratory, United Kingdom.
P D
PD
Lyne
P
P
Bamborough
D
D
Duncan
W G
WG
Richards
D000595:Amino Acid Sequence; D001665:Binding Sites; D018360:Crystallography, X-Ray; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000737:chemistry* / Q000378:metabolism; D013006:Growth Hormone / Q000737:chemistry; D006801:Humans; D007377:Interleukin-3 / Q000737:chemistry* / Q000378:metabolism; D008958:Models, Molecular; D008961:Models, Structural; D008969:Molecular Sequence Data; D017433:Protein Structure, Secondary; D016187:Receptors, Granulocyte-Macrophage Colony-Stimulating Factor / Q000737:chemistry* / Q000378:metabolism*; D016185:Receptors, Interleukin-3 / Q000737:chemistry* / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid
D007377:Interleukin-3; D016187:Receptors, Granulocyte-Macrophage Colony-Stimulating Factor; D016185:Receptors, Interleukin-3; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D013006:Growth Hormone