[Functional properties of a new line of immortalized human endothelial cells].
An immortalized human endothelial cell line was obtained by transfecting umbilical vein endothelial cells in primary culture with plasmid pMK16 containing SV40 replicated origin defective gene. The essential functional properties demonstrated in these immortalized human endothelial cells also retaining the classical phenotypical characteristics of endothelial cells in primary culture are: (1) endothelin-1 secretion; (2) capacity to convert big endothelin-1 into endothelin-1; (3) the capacity to secrete IL1 beta and IL6 interleukins both spontaneously and after lipopolysaccharide (LPS) stimulation; (4) arginine transfer from the extracellular to the intracellular medium. Such stable cell line could facilitate studies of regulation of endothelin-1 production; (5) No-synthase activity; (6) binding and metabolisation of acetylated low-density lipoproteins.
Laboratoire de biologie hormonale, hôpital Saint-Louis, Paris, France.
F
F
Moldovan
H R
HR
Soliman
H
H
Bennani
J
J
Dumas
D
D
Prévost
O
O
Cussenot
C
C
Mazières
G
G
Le Brun
C
C
Darbord
J
J
Fiet
D001120:Arginine / Q000493:pharmacokinetics; D016282:Aspartic Acid Endopeptidases / Q000378:metabolism; D002461:Cell Line, Transformed; D000072396:Endothelin-Converting Enzymes; D016232:Endothelins / Q000378:metabolism; D004730:Endothelium, Vascular / Q000166:cytology* / Q000502:physiology; D006801:Humans; D007375:Interleukin-1 / Q000737:chemistry; D015850:Interleukin-6 / Q000378:metabolism; D008077:Lipoproteins, LDL / Q000378:metabolism; D008666:Metalloendopeptidases; D010957:Plasmids / Q000235:genetics*; D013539:Simian virus 40 / Q000235:genetics*; D014162:Transfection; D014471:Umbilical Veins / Q000166:cytology* / Q000502:physiology*
D016232:Endothelins; D007375:Interleukin-1; D015850:Interleukin-6; D008077:Lipoproteins, LDL; D001120:Arginine; D016282:Aspartic Acid Endopeptidases; D008666:Metalloendopeptidases; D000072396:Endothelin-Converting Enzymes
Propriétés fonctionnelles d'une nouvelle lignée de cellules endothéliales humaines immortalisées.