Use of videocinematography to assess morphological qualities of conventionally cultured and cocultured embryos.
Videocinematography and image analysis procedures were utilized to evaluate the effect of conventional and coculture methodologies on morphological parameters in human embryos derived from in-vitro fertilization (IVF). Following 24-30 h of in-vitro development, cocultured embryos had more acceptable morphological features and less fragmentation present than embryos cultured in medium alone. Cocultured embryos were more advanced at the time of replacement when compared with conventionally cultured embryos. Zona pellucida variation (> or = 20%) also occurred more frequently in cocultured embryos. The morphological characteristic most enhanced after coculture was blastomere expansion. Patients who became pregnant across both culture treatments had a higher proportion of morphologically normal embryos replaced than patients who failed to achieve an ongoing pregnancy. Clinical pregnancy rate for patients following coculture was 49%, which was greater (P < 0.05) than the 29% detected for patients with embryos in the conventional culture group.
Department of Animal Science, Louisiana State University, Baton Rouge 70803, USA.
K
K
Morgan
K
K
Wiemer
N
N
Steuerwald
D
D
Hoffman
W
W
Maxson
R
R
Godke
D000818:Animals; D001757:Blastomeres / Q000502:physiology; D002417:Cattle; D018920:Coculture Techniques; D046508:Culture Techniques; D004624:Embryo Transfer; D004622:Embryo, Mammalian / Q000033:anatomy & histology* / Q000502:physiology; D005187:Fallopian Tubes / Q000502:physiology; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D011247:Pregnancy; D011256:Pregnancy Outcome; D014743:Videotape Recording; D015044:Zona Pellucida / Q000502:physiology