Stability of polypeptide immunoreactants and polyvinyl alcohol as a blocking agent on polyester cloth during dry storage.
During dry storage at 32 degrees C for 70 days, polypeptide immunoreactants (rabbit IgG and rabbit antiperoxidase antibody) adsorbed onto polyester cloth demonstrated no loss of immunoreactivity. Polyvinyl alcohol of molecular weight 30,000-70,000 provided a stable blocking capacity on the immunoreactant-adsorbed cloth without affecting immunoreactivity. Cloth-based enzyme immunoassay systems are suitable for field testing and transportation in the absence of refrigeration.
Boyd
S
S
Department of Biology, Carleton University, Ottawa, Ontario, Canada.
Yamazaki
H
H
D000327:Adsorption; D000818:Animals; D000906:Antibodies / Q000737:chemistry*; D006735:Horseradish Peroxidase / Q000276:immunology; D006813:Humidity; D007124:Immunoenzyme Techniques / Q000295:instrumentation*; D007074:Immunoglobulin G / Q000737:chemistry*; D011091:Polyesters; D011142:Polyvinyl Alcohol / Q000494:pharmacology*; D011309:Preservation, Biological / Q000379:methods*; D011817:Rabbits; D013784:Textiles
D000906:Antibodies; D007074:Immunoglobulin G; D011091:Polyesters; D011142:Polyvinyl Alcohol; D006735:Horseradish Peroxidase