[Prenatal determination of fetal rhesus factor in amnionic cells using polymerase chain reaction].
In Rhesus incompatibility, prenatal RhD typing of the fetus requires intrauterine blood sampling by cordocentesis or by chorionic villus biopsy. Amniocentesis is easier to perform, and carries a lower risk of enhancement of maternal immunization. Therefore, we evaluated polymerase chain reaction (PCR) for fetal RhD typing in amniocytes which were isolated from amniotic fluid (18-40 gw) obtained by amniocentesis (n=26) or during delivery (n=27). In the clinically most important group of children from RhD-negative women (n=25) and in 28 newborns of RhD- positive mothers, we found a 100 percent agreement between the findings of PCR and the results of serologic typing. If these encouraging results are confirmed in a larger series, the method could be used for the clinical management of RhD-negative women with Rhesus incompatibility and a heterozygous RhD-positive partner.
Universitäts-Frauenklinik Düsseldorf.
G
G
Crombach
F
F
Picard
M W
MW
Beckmann
D
D
Niederacher
H G
HG
Bender
D000649:Amniocentesis; D000653:Amniotic Fluid / Q000166:cytology*; D004899:Erythroblastosis, Fetal / Q000097:blood / Q000175:diagnosis*; D005260:Female; D005865:Gestational Age; D006801:Humans; D007231:Infant, Newborn; D016133:Polymerase Chain Reaction / Q000379:methods*; D011237:Predictive Value of Tests; D011247:Pregnancy; D012204:Rh-Hr Blood-Group System / Q000032:analysis*; D012703:Serotyping
D012204:Rh-Hr Blood-Group System; C026410:Rho(D) antigen
Pränatale Bestimmung des fetalen Rhesusfaktors in Amnionzellen mittels Polymerase-Ketten-Reaktion.