Alteration in the reactivity of sphingomyelin in mitogen-stimulated lymphocytes.
The antigens for a monoclonal antibody, VJ-41, established by alloimmunization of B10.A(3R) mice with lymphocytes from B10.A(5R) mice and screening of its reactivity toward Con A-stimulated human T lymphocytes, were found to be phosphorylcholine-containing ceramides (sphingomyelin) and disaturated fatty acyl glycerol (phosphatidyl-choline, PC), but neither deacylated sphingomyelin nor unsaturated fatty acid-containing PC reacted with the antibody. Although the reactivity of disaturated fatty acyl PC increased with increasing chain length, that of sphingomyelin was stronger than that of di-20:0-PC. The binding of the antibody to Con A-stimulated lymphocytes was inhibited by sphingomyelin-containing liposomes, but not by di-18:0-PC-containing ones, and the concentration of sphingomyelin in Con A-stimulated human T-lymphocytes was the same as that in non-stimulated ones, indicating that the reactivity of sphingomyelin in lymphocytes is altered by Con A-stimulation.
Department of Anesthesiology, Faculty of Medicine, University of Tokyo.
K
K
Hirota
K
K
Momoeda
K
K
Ono
K
K
Hanaoka
K
K
Horikawa
K
K
Okumura
M
M
Iwamori
D000818:Animals; D000911:Antibodies, Monoclonal; D000937:Antigen-Antibody Reactions; D002240:Carbohydrate Sequence; D003208:Concanavalin A / Q000494:pharmacology*; D000939:Epitopes; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008934:Mitogens / Q000494:pharmacology*; D008969:Molecular Sequence Data; D010767:Phosphorylcholine / Q000276:immunology*; D013109:Sphingomyelins / Q000097:blood*; D013268:Stimulation, Chemical
D000911:Antibodies, Monoclonal; D000939:Epitopes; D008934:Mitogens; D013109:Sphingomyelins; D010767:Phosphorylcholine; D003208:Concanavalin A