Calbindin D-28k immunoreactivity in the rat accessory olfactory bulb.
The distribution pattern and the morphology of calbindin D-28k-immunoreactive neurons were studied in the accessory olfactory bulb of the rat using a monoclonal antibody and the avidin-biotin-immunoperoxidase method. Positive neurons were observed in all layers but the vomeronasal nerve layer. Scarce mono-dendritic periglomerular neurons were calbindin D-28k-immunoreactive. Different morphological types of short-axon cells were calbindin D-28k-immunostained, with different degrees of intensity, in the boundary between the internal and external plexiform layer. In addition, deep short-axon cells located in the granule cell layer were calbindin D-28k-immunopositive. By contrast, previous studies described all cells in the rat accessory olfactory bulb as calbindin D-28k-immunonegative. The staining pattern in the rat accessory olfactory bulb showed both similarities and differences with the distribution pattern of the same calcium-binding protein in the main olfactory bulb.
Departamento de Biología Celular y Patología, Universidad de Salamanca, Spain.
A
A
Porteros
R
R
Arévalo
C
C
Crespo
E
E
García-Ojeda
J G
JG
Briñòn
J
J
Aijón
J R
JR
Alonso
D000818:Animals; D064026:Calbindins; D005260:Female; D007124:Immunoenzyme Techniques; D009419:Nerve Tissue Proteins / Q000032:analysis*; D009830:Olfactory Bulb / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D064030:S100 Calcium Binding Protein G / Q000032:analysis*
D064026:Calbindins; D009419:Nerve Tissue Proteins; D064030:S100 Calcium Binding Protein G