Immune response to heat-shock protein correlates with induction of insulitis in I-E alpha d transgenic NOD mice.
To evaluate the correlation between heat-shock protein (HSP) and insulitis, we compared lymphocyte proliferative response to Mycobacterium leprae HSP65 of NOD mice with that of I-E alpha d transgenic NOD (I-E+NOD) mice, which show no insulitis. We found that splenocytes from 15-week-old NOD mice showed a more marked proliferative response to HSP than did those from age-matched I-E+NOD mice (P < 0.05). We then transferred splenocytes from 12-week-old NOD mice into I-E+NOD mice to induce insulitis in the recipients and examined antibody levels against HSP. By 6 weeks posttransfer, insulitis was successfully transferred to four out of five recipients of NOD splenocytes and antibody levels against HSP were significantly higher in the NOD splenocyte-transferred group than in controls, which showed no insulitis (P < 0.01). These results suggest that immune response to HSP correlates with insulitis in NOD mice. Our results support the assertion that HSP is a useful antigen for investigating the etiology of IDDM.
Shimada
A
A
Department of Internal Medicine, Keio University School of Medicine, Tokyo, Japan.
Kasatani
T
T
Takei
I
I
Maruyama
T
T
Nomaguchi
H
H
Ozawa
Y
Y
Ishii
M
M
Kasuga
A
A
Tashiro
F
F
Miyazaki
J
J
Yamamura
K
K
Saruta
T
T
D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D001426:Bacterial Proteins / Q000276:immunology; D006360:Heat-Shock Proteins / Q000276:immunology*; D006358:Hot Temperature; D007249:Inflammation / Q000276:immunology; D007515:Islets of Langerhans / Q000276:immunology*; D008213:Lymphocyte Activation; D051379:Mice; D016688:Mice, Inbred NOD; D008822:Mice, Transgenic; D009166:Mycobacterium leprae
D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D006360:Heat-Shock Proteins