Automated determination of hypoxanthine and xanthine in urine by high-performance liquid chromatography with column switching.
We report a high-performance liquid chromatographic method with column switching for urinary hypoxanthine and xanthine. Analyses were carried out with both a reversed-phase column and an anion-exchange column connected by a column switch and controlled automatically by a computerized system controller. The relationships between standard concentrations and peak heights were linear in a concentration range of 1 to 1000 nmol/ml. The recovery of hypoxanthine added to urine was 101.1%, and that of xanthine was 98.1%. With our method urinary hypoxanthine and xanthine can be measured accurately without any sample preparation other than filtration.
Department of Pediatrics, Nagoya City Child Welfare Center, Japan.
S
S
Sumi
K
K
Kidouchi
S
S
Ohba
Y
Y
Wada
D000328:Adult; D001322:Autoanalysis; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007042:Hypoxanthines / Q000652:urine*; D012016:Reference Values; D013056:Spectrophotometry, Ultraviolet; D014970:Xanthines / Q000652:urine*
D007042:Hypoxanthines; D014970:Xanthines