Effect of sulfamethazine on mixed function oxidase in chickens.
Sulfamethazine (SMZ) ip administration for 3 d to chickens showed significant induction of cytochrome P-450 levels and in the activities of aminopyrine N-demethylase, aniline hydroxylase and glutathione s-transferase at the dosage of 150 mg SMZ/kg body weight. Cytochrome P-450 and the activity of aniline hydroxylase were significantly decreased at 300 mg SMZ/kg body weight when compared to 150 mg SMZ/kg body weight. Other doses produced no significant changes in the parameters studied. One hundred fifty mg SMZ/kg for 1 or 3 d caused significant induction of cytochrome b5, cytochrome P-450, the drug metabolizing enzymes and glutathione s-transferase; however, 5 d of SMZ produced no significant changes. The in vitro 10 mM SMZ inhibition of aminopyrine N-demethylase and aniline hydroxylase and the induction of enzymes at 150 mg SMZ/kg body weight indicates that SMZ is a substrate of the mixed function oxidase system and may be an inducer of specific forms of cytochrome P-450.
Kodam
K M
KM
Department of Chemistry, Shivaji University, Kolhapur, India.
Govindwar
S P
SP
D000633:Aminopyrine N-Demethylase / Q000187:drug effects / Q000378:metabolism; D000815:Aniline Hydroxylase / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D000890:Anti-Infective Agents / Q000008:administration & dosage / Q000633:toxicity*; D002645:Chickens; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis / Q000187:drug effects / Q000378:metabolism; D004790:Enzyme Induction / Q000187:drug effects; D005982:Glutathione Transferase / Q000187:drug effects / Q000378:metabolism*; D008862:Microsomes, Liver / Q000187:drug effects* / Q000201:enzymology; D006899:Mixed Function Oxygenases / Q000187:drug effects / Q000378:metabolism*; D013418:Sulfamethazine / Q000008:administration & dosage / Q000633:toxicity*
D000890:Anti-Infective Agents; D013418:Sulfamethazine; D003577:Cytochrome P-450 Enzyme System; D006899:Mixed Function Oxygenases; D000815:Aniline Hydroxylase; D000633:Aminopyrine N-Demethylase; D005982:Glutathione Transferase