Identification of double Holliday junctions as intermediates in meiotic recombination.
During meiosis, branched DNA molecules containing information from both parental chromosomes occur in vivo at loci where meiosis-specific double-stranded breaks occur. We demonstrate here that these joint molecules are recombination intermediates: they contain single strands that have undergone exchange of information. Moreover, these joint molecules are resolved into both parental and recombinant duplexes when treated in vitro with Holliday junction-resolving endonucleases RuvC or T4 endo VII. Taken together with previous observations, these results strongly suggest that joint molecules are double Holliday junctions.
Schwacha
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Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA.
Kleckner
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N
D000429:Alcohol Oxidoreductases; D000619:Aminohydrolases; D001426:Bacterial Proteins; D003432:Cross-Linking Reagents; D003434:Crossing Over, Genetic / Q000235:genetics; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D004277:DNA, Single-Stranded / Q000737:chemistry / Q000235:genetics*; D004706:Endodeoxyribonucleases; D029968:Escherichia coli Proteins; D005363:Ficusin; D005656:Fungal Proteins / Q000235:genetics; D008540:Meiosis / Q000235:genetics*; D008957:Models, Genetic; D009690:Nucleic Acid Conformation; D009692:Nucleic Acid Heteroduplexes / Q000235:genetics; D011755:Pyrophosphatases; D011995:Recombination, Genetic / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors / Q000235:genetics
D001426:Bacterial Proteins; D003432:Cross-Linking Reagents; D004271:DNA, Fungal; D004277:DNA, Single-Stranded; D029968:Escherichia coli Proteins; D005656:Fungal Proteins; D009692:Nucleic Acid Heteroduplexes; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; C069806:ruvC protein, E coli; D000429:Alcohol Oxidoreductases; C092116:HIS4 protein, S cerevisiae; D004706:Endodeoxyribonucleases; C035571:endodeoxyribonuclease VII; D000619:Aminohydrolases; D011755:Pyrophosphatases; D005363:Ficusin