The contraction of small arteries in the perimetrium by presurgical medication with the luteinizing hormone-releasing hormone agonist to patients with leiomyomas: an electron and immunoelectron microscopy.
The remarkable contraction of small arteries occurred in the perimetrium of the uteri with leiomyomas by a presurgical medication P6th the luteinizing hormone-releasing hormone (LHRH) agonist (buserelin). The contracted vessels showed a significant increase in number of the Weibel-Palade (WP) bodies which occasionally underwent the degranulation and the discharge in a manner of exocytosis. By immunoelectron microscopy, localizations of endothelin (ET)-1 immunoreactive gold particles on the WP bodies as well as on cisterns of the rough endoplasmic reticulum (rER) and Golgi apparatus were pronounced in the buserelin-medicated group when compared to the non-medicated one. These findings indicate that the increase of the WP bodies storing ET-1, of which synthesis in the rER-Golgi system enhances, predominantly occurred in the small arteries in the perimetrium by the buserelin-medication prior to the hysterectomy. ET-1, released into the subendothelial layer, seems to play an important role in the contraction of the vascular media in a manner of paracrine.
Department of Obstetrics and Gynecology, University of Occupational and Environmental Health, School of Medicine, Kitakyushu.
K
K
Hamasaki
Y
Y
Doi
Y
Y
Sakamoto
M
M
Kashimura
S
S
Fujimoto
D000328:Adult; D001158:Arteries / Q000648:ultrastructure; D002064:Buserelin / Q000008:administration & dosage / Q000494:pharmacology*; D016889:Endometrial Neoplasms / Q000601:surgery / Q000648:ultrastructure; D004717:Endometrium / Q000098:blood supply*; D016232:Endothelins / Q000378:metabolism / Q000502:physiology; D004730:Endothelium, Vascular / Q000378:metabolism / Q000648:ultrastructure; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000819:agonists*; D006801:Humans; D002479:Inclusion Bodies / Q000378:metabolism / Q000648:ultrastructure; D007889:Leiomyoma / Q000601:surgery / Q000648:ultrastructure; D008854:Microscopy, Electron; D016253:Microscopy, Immunoelectron; D008875:Middle Aged; D011292:Premedication; D014661:Vasoconstriction / Q000187:drug effects*
D016232:Endothelins; D007987:Gonadotropin-Releasing Hormone; D002064:Buserelin