Evaluation of water molecules in the cold-preserved rat liver by proton magnetic resonance imaging.
Hypothermically preserved rat livers were studied with proton magnetic resonance imaging (1H-MRI) under proton density-, spin-lattice relaxation time-, spin-spin relaxation time- and diffusion-weighted (P-W, T1-W, T2-W and D-W) conditions. Relative signal intensities (RSI) of the liver to distilled water in terms of P-W, T1-W, T2-W and D-W increased time-dependently during 12 h hypothermic (4 degrees C) preservation with saline, while these parameters did not increase during preservation with University of Wisconsin (UW) solution. One-hour Wiggers' hypotensive treatment before the harvesting increased the RSIs of P-W, T2-W and D-W, and the subsequent 12-hour preservation with UW solution did not improve the increased RSIs. These results suggest that 1H-MRI has potential application in evaluating the biophysical changes of water molecules in the liver graft, which were measured by placing the harvested liver in a plastic bag under a magnetic field at a low temperature.
Second Department of Surgery, Faculty of Medicine, Kyoto University, Japan.
N
N
Yanabu
A
A
Tanaka
B
B
Sato
S
S
Mori
T
T
Kitai
A
A
Tokuka
T
T
Inomoto
Y
Y
Yamaoka
S
S
Morikawa
T
T
Inubushi
D000241:Adenosine / Q000494:pharmacology; D000493:Allopurinol / Q000494:pharmacology; D000818:Animals; D015925:Cryopreservation; D005978:Glutathione / Q000494:pharmacology; D007022:Hypotension / Q000378:metabolism; D066298:In Vitro Techniques; D007328:Insulin / Q000494:pharmacology; D008099:Liver / Q000033:anatomy & histology / Q000378:metabolism*; D008279:Magnetic Resonance Imaging; D008297:Male; D019188:Organ Preservation Solutions; D011522:Protons; D011887:Raffinose / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D012771:Shock, Hemorrhagic / Q000378:metabolism; D012965:Sodium Chloride / Q000494:pharmacology; D013997:Time Factors; D014867:Water / Q000378:metabolism*
D007328:Insulin; D019188:Organ Preservation Solutions; D011522:Protons; C055206:University of Wisconsin-lactobionate solution; D014867:Water; D012965:Sodium Chloride; D000493:Allopurinol; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose