Murine IgG subclass antibodies to antigens incorporated in liposomes containing lipid A.
The IgG subclass responses to antigens incorporated in liposomes containing lipid A were investigated using a synthetic malarial antigen (SPf66) and cholera toxin (CT). The antigen-specific IgG subclass response was determined in BALB/c mice immunized with either: (a) SPf66 encapsulated in liposomes containing lipid A, (b) CT bound to the surface of liposomes containing lipid A, or (c) both encapsulated SPf66 and surface-bound CT in the same liposomes. In each case the antibodies to SPf66, CT and lipid A demonstrated an IgG2a predominance. Liposomes containing lipid A not only increased the magnitude of the antibody response to liposomal antigens but elicited predominantly IgG2a subclass antibodies as well.
Department of Membrane Biochemistry, Walter Reed Army Institute of Research, Washington, DC 20307-5100, USA.
G M
GM
Glenn
M
M
Rao
R L
RL
Richards
G R
GR
Matyas
C R
CR
Alving
D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis; D000913:Antibodies, Protozoan / Q000096:biosynthesis; D002772:Cholera Toxin / Q000276:immunology; D003429:Cross Reactions; D007074:Immunoglobulin G / Q000096:biosynthesis* / Q000145:classification*; D008050:Lipid A / Q000276:immunology*; D008081:Liposomes / Q000276:immunology*; D017780:Malaria Vaccines / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D015800:Protozoan Proteins / Q000276:immunology; D011994:Recombinant Proteins
D000907:Antibodies, Bacterial; D000913:Antibodies, Protozoan; D007074:Immunoglobulin G; D008050:Lipid A; D008081:Liposomes; D017780:Malaria Vaccines; D015800:Protozoan Proteins; D011994:Recombinant Proteins; C066269:SPf66 protein, Plasmodium; D002772:Cholera Toxin