An antigen detection immunoassay for big liver and spleen disease agent.
An enzyme-linked immunosorbent assay (ELISA) able to detect an antigen associated with infection with big liver and spleen (BLS) agent was developed. The assay is a capture ELISA (C-ELISA) procedure using ELISA plates coated with mouse monoclonal antibody (mAb 1H4) for antigen capture and a polyclonal chicken Ig preparation, extracted from yolk of BLS antibody positive birds, for detection of captured BLS antigen. mAb 1H4 was produced to a partially purified antigen preparation from liver suspensions of naturally BLS infected birds. The C-ELISA was evaluated for specificity and sensitivity in naturally and experimentally BLS infected and uninfected broiler and layer breeds of domestic fowl.
Animal Industries Division, Department of Agriculture, South Perth, WA, Australia.
T M
TM
Ellis
C J
CJ
Payne
S L
SL
Plant
A R
AR
Gregory
D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D000956:Antigens, Viral / Q000032:analysis*; D002645:Chickens; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary; D005260:Female; D006529:Hepatomegaly / Q000662:veterinary* / Q000821:virology; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology; D011201:Poultry Diseases / Q000821:virology*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013163:Splenomegaly / Q000662:veterinary* / Q000821:virology; D014777:Virus Diseases / Q000175:diagnosis / Q000662:veterinary*
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral