Cell-free assay system for Ras-dependent MEK activation.
Kuroda
S
S
Department of Molecular Biology and Biochemistry, Osaka University Medical School, Okazaki, Japan.
Shimizu
K
K
Yamamori
B
B
Takai
Y
Y
D000818:Animals; D016367:Baculoviridae / Q000235:genetics; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000302:isolation & purification / Q000378:metabolism; D002474:Cell-Free System; D004789:Enzyme Activation; D048049:Extracellular Signal-Regulated MAP Kinases; D005260:Female; D006160:Guanosine Triphosphate / Q000378:metabolism; D066298:In Vitro Techniques; D008055:Lipids / Q000737:chemistry; D048369:MAP Kinase Kinase 1; D020930:MAP Kinase Kinase Kinases; D051379:Mice; D020929:Mitogen-Activated Protein Kinase Kinases; D009865:Oocytes / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D011505:Protein-Tyrosine Kinases / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D018411:Spodoptera; D029867:Xenopus Proteins; D014982:Xenopus laevis; D018631:ras Proteins / Q000737:chemistry / Q000378:metabolism*
D008055:Lipids; D011994:Recombinant Proteins; D029867:Xenopus Proteins; D006160:Guanosine Triphosphate; D011505:Protein-Tyrosine Kinases; D017346:Protein Serine-Threonine Kinases; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D048049:Extracellular Signal-Regulated MAP Kinases; D020930:MAP Kinase Kinase Kinases; C499530:REKS protein, Xenopus; C089319:Ras-dependent mitogen-activated protein kinase kinase kinase; D048369:MAP Kinase Kinase 1; C482127:Map2k1 protein, mouse; D020929:Mitogen-Activated Protein Kinase Kinases; D018631:ras Proteins