Molecular cloning and gene mapping of human basic and acidic calponins.
The nucleotide and deduced amino acid sequences of human basic and acidic calponins were determined. The basic calponin cDNA from human aorta (1496 bp) contained a single open reading frame (ORF) which encodes 297 amino acids (33,169 Da). The acidic calponin cDNA from human kidney (1607 bp) contained a single ORF which encodes 329 amino acids (36,412 Da). Basic calponin mRNA was expressed in only smooth muscle tissues, but acidic calponin mRNA was expressed in non-smooth muscle tissues as well as smooth muscle tissues. Fluorescent in situ hybridization revealed that basic and acidic calponin genes localize in 19p13.1-13.2 and 1p21-22 of human chromosomes, respectively.
Second Department of Internal Medicine, Ehime University School of Medicine, Japan.
M
M
Maguchi
W
W
Nishida
K
K
Kohara
A
A
Kuwano
I
I
Kondo
K
K
Hiwada
D000595:Amino Acid Sequence; D001011:Aorta / Q000378:metabolism; D001483:Base Sequence; D002135:Calcium-Binding Proteins / Q000737:chemistry / Q000235:genetics*; D002874:Chromosome Mapping; D002878:Chromosomes, Human, Pair 1 / Q000235:genetics; D002888:Chromosomes, Human, Pair 19 / Q000235:genetics; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007668:Kidney / Q000378:metabolism; D008840:Microfilament Proteins; D008969:Molecular Sequence Data; D009130:Muscle, Smooth / Q000378:metabolism; D016366:Open Reading Frames; D014018:Tissue Distribution; D000096985:Calponins
D002135:Calcium-Binding Proteins; D017931:DNA Primers; D018076:DNA, Complementary; D008840:Microfilament Proteins; D000096985:Calponins