T cell antigenic and neuritogenic activity of recombinant human peripheral myelin P2 protein.
The major neuritogenic protein of peripheral nerve myelin is the P2 protein. Human P2 is a candidate autoantigen in inflammatory demyelinating diseases of the peripheral nervous system. Since human P2 is not readily available, we produced full-length recombinant human P2 protein (rhP2) in Escherichia coli. RhP2 was recognized by neuritogenic rat T cell lines and induced experimental autoimmune neuritis in Lewis rats. Production of rhP2 allowed the generation of human T cell lines reactive to the autologous protein. Studies of human T cell autoreactivity as well as efforts to use hP2 as a tolerogen will be facilitated by the large-scale expression of rhP2.
Department of Neurology, Bayerische Julius-Maximilians-Universität, Würzburg, Germany.
A
A
Weishaupt
G
G
Giegerich
S
S
Jung
R
R
Gold
U
U
Enders
M
M
Pette
K
K
Hayasaka
H P
HP
Hartung
K V
KV
Toyka
D000595:Amino Acid Sequence; D000818:Animals; D001323:Autoantibodies; D001483:Base Sequence; D004926:Escherichia coli / Q000235:genetics; D005260:Female; D006801:Humans; D007114:Immunization; D008969:Molecular Sequence Data; D018996:Myelin P2 Protein / Q000276:immunology* / Q000302:isolation & purification; D009443:Neuritis / Q000276:immunology; D017933:Peripheral Nervous System / Q000276:immunology; D051381:Rats; D011917:Rats, Inbred Lew; D011994:Recombinant Proteins / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
D001323:Autoantibodies; D018996:Myelin P2 Protein; D011994:Recombinant Proteins