Effect of long and short fibre amosite asbestos on in vitro TNF production by rat alveolar macrophages: the modifying effect of lipopolysaccharide.
The influence of long and short fibre amosite on the generation of tumor necrosis factor (TNF) by rat alveolar macrophages was investigated in vitro. TNF rich supernatants were prepared from macrophages cultured in F10 medium +2% Bovine Serum Albumin (BSA). Spontaneously released TNF from unstimulated macrophages and TNF rich supernatants from macrophages exposed to Lipopolysaccharide (LPS) and fibres were stored at -70 degrees C and then tested for their cytotoxicity towards L929 cells. Maximum spontaneously released TNF was obtained from 24 hour macrophage cultures. Short amosite fibres had no significant effect in stimulating alveolar macrophages to release TNF while the 50 micrograms dose of long fibres resulted in significantly increased release of TNF. Cotreatment of alveolar macrophages with LPS and fibres further enhanced the TNF production and maximum production was obtained with LPS +50 micrograms dose of long fibre resulted in significantly increased release of TNF. Co-treatment of alveolar macrophages with LPS and fibres further enhanced the TNF production and maximum production was obtained with LPS +50 micrograms of long fibre amosite. The present study indicates that fibre dimension is a major factor in in vitro dust activity and TNF has a possible active role to play in dust induced inflammation in vivo.
Dogra
S
S
Industrial Toxicology Research Centre, Mahatma Gandhi Marg, Lucknow, India.
Donaldson
K
K
D000818:Animals; D017639:Asbestos, Amosite / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D005260:Female; D007739:L Cells; D008070:Lipopolysaccharides / Q000494:pharmacology*; D016676:Macrophages, Alveolar / Q000378:metabolism*; D051379:Mice; D051381:Rats; D017208:Rats, Wistar; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D017639:Asbestos, Amosite