Mouse microglial cell lines differing in constitutive and interferon-gamma-inducible antigen-presenting activities for naive and memory CD4+ and CD8+ T cells.
We developed a panel of non-virus transformed cell lines derived from individual microglial precursors residing in the brains of normal mice. These colony stimulating factor-1-dependent cell lines are B7-1+ (CD80), Mac-1+, Mac-2+, Mac-3+, CD45+, MHC class I+, colony stimulating factor-1 receptor+, and they ingest antibody-coated particles. However, the cell lines differ in their expression of B7-2 (CD86), F4/80, Ly-6C and MHC class II molecules. They also differ in their ability to constitutively process and present antigens to naive CD4+ and CD8+ T cells, memory CD4+ and CD8+, and in the manner by which interferon gamma modulates their antigen-presenting activities. These cell lines should be valuable as models for studies on the immunobiology of the microglia.
Department of Immunology, St. Jude Children's Research Hospital, Memphis, TN 3810, USA.
W S
WS
Walker
J
J
Gatewood
E
E
Olivas
D
D
Askew
C E
CE
Havenith
D000276:Adjuvants, Immunologic; D000818:Animals; D017951:Antigen Presentation / Q000276:immunology*; D000956:Antigens, Viral / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D002460:Cell Line / Q000166:cytology / Q000276:immunology; D006433:Hemocyanins; D006825:Hybridomas; D007156:Immunologic Memory / Q000276:immunology; D007371:Interferon-gamma / Q000276:immunology*; D007519:Isoantigens / Q000276:immunology; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D017628:Microglia / Q000737:chemistry / Q000166:cytology* / Q000276:immunology; D010587:Phagocytosis / Q000276:immunology; D010641:Phenotype
D000276:Adjuvants, Immunologic; D000956:Antigens, Viral; D007519:Isoantigens; D007371:Interferon-gamma; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin