Sequence, initial functional analysis and protein-DNA binding sites of the mouse beta B2-crystallin-encoding gene.
An 800-bp fragment of genomic DNA upstream from the origin of transcription of the mouse beta B2-crystallin-encoding gene (beta B2-Cry) has been isolated and its nucleotide sequence determined. Promoter fragments 275 to +30 or -110 to +30, fused to cat reporter gene, activated transcription in transiently transfected rabbit lens epithelial cells, but not in various non-lens cells. The beta B2-Cry mouse promoter contains a typical TATA-box located approx. 25 bp upstream from the transcription start point. Binding sites (upstream from the TATA-box) for transcription factors possibly involved in the regulation of gene expression have been identified by DNaseI footprinting analysis and lens cell nuclear extracts. Most notably is the binding of the Pax-6 paired domain (PrD) which correlates with the binding of lens cell nuclear proteins at the -80 to -40 region.
Laboratory of Mechanisms of Ocular Diseases, National Eye Institute, National Institutes of Health, Bethesda, MD 20892, USA.
C
C
Chambers
A
A
Cvekl
C M
CM
Sax
P
P
Russell
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002478:Cells, Cultured; D003459:Crystallins / Q000235:genetics*; D017931:DNA Primers / Q000737:chemistry; D004268:DNA-Binding Proteins / Q000378:metabolism; D003850:Deoxyribonuclease I; D005136:Eye Proteins; D018398:Homeodomain Proteins; D051379:Mice; D008969:Molecular Sequence Data; D000071841:PAX6 Transcription Factor; D051761:Paired Box Transcription Factors; D011401:Promoter Regions, Genetic; D011817:Rabbits; D012045:Regulatory Sequences, Nucleic Acid; D012097:Repressor Proteins; D014157:Transcription Factors / Q000378:metabolism
D003459:Crystallins; D017931:DNA Primers; D004268:DNA-Binding Proteins; D005136:Eye Proteins; D018398:Homeodomain Proteins; D000071841:PAX6 Transcription Factor; D051761:Paired Box Transcription Factors; C000605732:Pax6 protein, mouse; D012097:Repressor Proteins; D014157:Transcription Factors; D003850:Deoxyribonuclease I