Aberrant splicing of rat steroid 17 alpha-hydroxylase transcripts.
Polymerase chain reaction amplification of the cDNA encoding steroid 17 alpha-hydroxylase (P450c17) demonstrated very low levels of this transcript and a shorter variant of still lower abundance in rat adrenals and brain. Sequence analysis of the two amplified products revealed that the shorter variant resulted from a deletion of the second exon which does not maintain the open reading frame, suggesting that faulty splicing gave rise to this minor species.
Fidia-Georgetown Institute for the Neurosciences, Georgetown University School of Medicine, Washington, DC 20007, USA.
J L
JL
Sanne
K E
KE
Krueger
D000311:Adrenal Glands / Q000737:chemistry; D000446:Aldehyde-Lyases / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D001923:Brain Chemistry; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D005091:Exons / Q000235:genetics; D008297:Male; D008969:Molecular Sequence Data; D009928:Organ Specificity; D016133:Polymerase Chain Reaction / Q000379:methods; D012326:RNA Splicing; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics*; D051381:Rats; D017422:Sequence Analysis, DNA; D017384:Sequence Deletion; D013254:Steroid 17-alpha-Hydroxylase; D013737:Testis / Q000737:chemistry / Q000201:enzymology
D012333:RNA, Messenger; D003577:Cytochrome P-450 Enzyme System; D013254:Steroid 17-alpha-Hydroxylase; D000446:Aldehyde-Lyases