A new approach to methotrexate and lipiodol suspensions for ectopic pregnancy: preliminary in vitro and animal experiments.
OBJECTIVE
To measure and evaluate the in vitro release of MTX from suspensions of methotrexate (MTX) and lipiodol (LPD), and to investigate the MTX concentration and fertility rate using an animal model.


METHODS
This study was divided into three components: a) An in vitro Release Study, b) Animal Study No. 1 (concentration of tissue and plasma levels), and c) Animal Study No. 2 (fertility rate after MTX suspension).


RESULTS
The releasing rate in vitro was more rapid with MTX-S (MTX dissolved in saline) than with either MTX-LPD (MTX dissolved in lipiodol) or MTX-LPD-PC (MTX suspended in lipiodol and phosphatidylcholin, i.e., MTX suspension). The tissue concentration tended to be higher with an MTX suspension than with MTX-S. There was no significant difference in the fertility rate or the nidation index among the 3 groups (Groups 1, 2, and 3).


CONCLUSIONS
The injection of an MTX suspension is useful for increasing the tissue concentration and maintaining the long-term effectiveness of MTX, and this technique might offer a new approach in the treatment of ectopic pregnancy (EP) or in second-line therapy for persistent EP.
Department of Obstetrics and Gynecology, Nagasaki University School of Medicine, Japan.
A
A
Fujishita
T
T
Ishimaru
H
H
Masuzaki
T
T
Matsuwaki
R O
RO
Chavez
T
T
Yamabe
M
M
Ogasawara
D000818:Animals; D005260:Female; D005298:Fertility; D007459:Iodized Oil / Q000008:administration & dosage / Q000493:pharmacokinetics* / Q000627:therapeutic use; D008727:Methotrexate / Q000008:administration & dosage / Q000493:pharmacokinetics* / Q000627:therapeutic use; D011247:Pregnancy; D011271:Pregnancy, Ectopic / Q000188:drug therapy*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013535:Suspensions
D013535:Suspensions; D007459:Iodized Oil; D008727:Methotrexate