Experimental studies of some microrheological factors in serious brain ischemia after stobadine pretreatment.
Serious brain ischemia was induced by occlusion of cerebral arteries in dogs. The occlusion time was 7 min. The blood was collected at various intervals of reperfusion (5, 60, 180, 240 min and 24 h). Thirty minutes before ischemization, stobadine was given (1, 2, or 5 mg/kg). The changes of erythrocyte membrane fluidity were evaluated using colloid-osmotic hemolysis induced by brilliant cresyl blue. In the control group (without stobadine) the colloid-osmotic hemolysis was significantly increased immediately after ischemization and after 5 and 60 min. However, after 240 min of reperfusion, a significant decrease of hemolysis was observed. The increase of colloid-osmotic hemolysis after ischemization in the control group was prevented after stobadine pretreatment. The thrombotization of microcirculation that was observed in the control group was not present after stobadine pretreatment.
Department of Histology and Embryology, Medical Faculty University P.J. Safarik, Kosice, Slovak Republic.
M
M
Pomfy
A
A
Nicák
J
J
Mojzis
J
J
Húska
L
L
Benes
D000818:Animals; D001809:Blood Viscosity / Q000187:drug effects; D002545:Brain Ischemia / Q000503:physiopathology*; D002243:Carbolines / Q000494:pharmacology*; D002540:Cerebral Cortex / Q000098:blood supply; D002560:Cerebrovascular Circulation / Q000502:physiology; D004285:Dogs; D004907:Erythrocyte Deformability / Q000187:drug effects / Q000502:physiology; D004910:Erythrocyte Membrane / Q000502:physiology; D005260:Female; D006461:Hemolysis / Q000502:physiology; D008297:Male; D008560:Membrane Fluidity / Q000502:physiology; D012039:Regional Blood Flow / Q000502:physiology; D015424:Reperfusion; D014665:Vasodilator Agents / Q000494:pharmacology*
D002243:Carbolines; D014665:Vasodilator Agents; C100106:dicarbine