Host-mediated modification of PvuII restriction in Mycobacterium tuberculosis.
Restriction endonuclease PvuII plays a central role in restriction fragment length polymorphism analysis of Mycobacterium tuberculosis complex isolates with IS6110 as a genetic marker. We have investigated the basis for an apparent dichotomy in PvuII restriction fragment pattersn observed among strains of the M. tuberculosis complex. The chromosomal regions of two modified PvuII restriction sites, located upstream of the katG gene and downstream of an IS1081 insertion sequence, were studied in more detail. An identical 10-bp DNA sequence (CAGCTGGAGC) containing a PvuII site was found in both regions, and site-directed mutagenesis analysis revealed that this sequence was a target for modification. Strain-specific modification of PvuII sites was identified in DNA from over 80% of the nearly 800 isolates examined. Furthermore, the proportion of modifying and nonmodifying strains differs significantly from country to country.
van Soolingen
D
D
Laboratory for Bacteriology and Antimicrobial Agents, National Institute of Public Health and Environmental Protection, Bilthoven, The Netherlands.
de Haas
P E
PE
Blumenthal
R M
RM
Kremer
K
K
Sluijter
M
M
Pijnenburg
J E
JE
Schouls
L M
LM
Thole
J E
JE
Dessens-Kroon
M W
MW
van Embden
J D
JD
Hermans
P W
PW
D001426:Bacterial Proteins; D001483:Base Sequence; D015342:DNA Probes; D004251:DNA Transposable Elements / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics* / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009169:Mycobacterium tuberculosis / Q000235:genetics*; D010544:Peroxidases / Q000235:genetics; D012150:Polymorphism, Restriction Fragment Length; D013045:Species Specificity
D001426:Bacterial Proteins; D015342:DNA Probes; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D010544:Peroxidases; C076136:catalase HPI; C024669:endodeoxyribonuclease AluI; C025630:CAGCTG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific