Isolation, characterization, and chromosomal localization of mouse and human COUP-TF I and II genes.
Chicken ovalbumin upstream promoter transcription factors (COUP-TFs) are orphan members of the steroid/thyroid hormone receptor superfamily. COUP-TF homologues have been cloned in many species, from Drosophila to human. The protein sequences of COUP-TFs are highly homologous across species, suggesting functional conservation. Two COUP-TF genes have been cloned from human, and their genomic organizations have been characterized. To determine whether the genomic organization is conserved between human and mouse, we isolated two mouse COUP-TF genes (I and II) and characterized their genomic structures. Both genes have relatively simple structures that are similar to those of their human counterparts. In addition, we mapped mouse COUP-TF I to the distal region of chromosome 13 and COUP-TF II to the central region of chromosome 7. Furthermore, we mapped human COUP-TF I to 5q14 of chromosome 5 and COUP-TF II to 15q26 of chromosome 15. The results demonstrate that COUP-TF genes are located in chromosomal regions that are syntenic between mouse and human.
Department of Cell Biology, Baylor College of Medicine, Houston, Texas 77030, USA.
Y
Y
Qiu
V
V
Krishnan
Z
Z
Zeng
D J
DJ
Gilbert
N G
NG
Copeland
L
L
Gibson
T
T
Yang-Feng
N A
NA
Jenkins
M J
MJ
Tsai
S Y
SY
Tsai
D000818:Animals; D001483:Base Sequence; D051838:COUP Transcription Factor I; D051836:COUP Transcription Factors; D002645:Chickens; D002874:Chromosome Mapping; D002884:Chromosomes, Human, Pair 15; D002895:Chromosomes, Human, Pair 5; D003001:Cloning, Molecular; D003433:Crosses, Genetic; D004268:DNA-Binding Proteins / Q000235:genetics*; D005091:Exons; D005260:Female; D015186:Hominidae / Q000235:genetics*; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL / Q000235:genetics*; D008969:Molecular Sequence Data; D009115:Muridae / Q000235:genetics*; D010047:Ovalbumin / Q000235:genetics; D011401:Promoter Regions, Genetic; D011956:Receptors, Cell Surface / Q000235:genetics; D011965:Receptors, Glucocorticoid / Q000235:genetics; D011987:Receptors, Steroid; D013045:Species Specificity; D014157:Transcription Factors / Q000235:genetics*
D051838:COUP Transcription Factor I; D051836:COUP Transcription Factors; D004268:DNA-Binding Proteins; C497589:NR2F1 protein, human; C497590:Nr2f1 protein, mouse; D011956:Receptors, Cell Surface; D011965:Receptors, Glucocorticoid; D011987:Receptors, Steroid; D014157:Transcription Factors; D010047:Ovalbumin