Post-transcriptional transactivation of human retroviral envelope glycoprotein expression by herpes simplex virus Us11 protein.
Herpes simplex virus type 1 (HSV-1) Us11 protein, a true late gene product packaged within the virion, is delivered into cells after infection, exhibits a nucleocytoplasmic localization at early times, and later accumulates in the nucleoli. This RNA-binding basic phosphoprotein, capable of oligomerization, is supposed to be involved in post-transcriptional regulation of gene expression after HSV-1 infection. Expression of human T-cell leukaemia/lymphoma virus type-I (HTLV-I) and of human immunodeficiency virus type 1 (HIV-1) is post-transcriptionally regulated by Rex and Rev, respectively. These proteins are required for the cytoplasmic expression of unspliced gag-pol and singly spliced env transcripts. Here we show that HSV-1 Us11 protein is able to bind Rex- and Rev-responsive elements and to transactivate envelope retroviral glycoprotein expression.
Université Claude Bernard, Lyon-1/CNRS UMR30, Faculté de Médecine, Lyon, France.
J J
JJ
Diaz
M D
MD
Dodon
N
N
Schaerer-Uthurralt
D
D
Simonin
K
K
Kindbeiter
L
L
Gazzolo
J J
JJ
Madjar
D003593:Cytoplasm / Q000378:metabolism; D015967:Gene Expression Regulation, Viral; D015694:Gene Products, rev / Q000235:genetics / Q000502:physiology; D016370:Gene Products, rex / Q000235:genetics / Q000502:physiology; D016356:Gene Products, tax / Q000235:genetics; D015726:Giant Cells / Q000821:virology; D015497:HIV-1 / Q000235:genetics*; D006367:HeLa Cells; D018259:Herpesvirus 1, Human / Q000235:genetics*; D015368:Human T-lymphotropic virus 1 / Q000235:genetics*; D006801:Humans; D012333:RNA, Messenger / Q000378:metabolism; D012367:RNA, Viral / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000235:genetics / Q000502:physiology*; D015534:Trans-Activators / Q000235:genetics / Q000502:physiology*; D014162:Transfection; D014759:Viral Envelope Proteins / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics / Q000502:physiology*; D054321:rev Gene Products, Human Immunodeficiency Virus
D015694:Gene Products, rev; D016370:Gene Products, rex; D016356:Gene Products, tax; D012333:RNA, Messenger; D012367:RNA, Viral; D016601:RNA-Binding Proteins; D015534:Trans-Activators; C074546:US11 protein, herpesvirus; D014759:Viral Envelope Proteins; D014764:Viral Proteins; D054321:rev Gene Products, Human Immunodeficiency Virus