The effect of heparin on Cu(2+)-mediated oxidation of human low-density lipoproteins.
The effect of heparin (HE) on the susceptibility of human low-density lipoprotein (LDL) to Cu(2+)-induced oxidation was investigated by monitoring conjugated diene formation. HE did not modify the maximum formation of conjugated diene, but increased markedly the lag phase. The plot of change in oxidation rate vs. time showed that the absolute value of Vmax was dependent on Cu2+ concentration and that HE increased the time necessary to reach Vmax. The value of constant K (the Cu2+ concentration producing a tlag of twice the minimum value) increased in the presence of HE, whereas the value of tmin (the time theoretically required for LDL oxidation at an infinite Cu2+ concentration) was not substantially affected. These results indicate that HE might play a protective antioxidant effect on LDL, probably affecting both the structural properties of the particle and the amount of Cu2+ available for the oxidation.
Department of Biochemistry A. Castellani, University of Pavia, Italy.
R
R
Albertini
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S
Rindi
A
A
Passi
G
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Palladini
G
G
Pallavicini
G
G
De Luca
D000975:Antioxidants / Q000494:pharmacology*; D002413:Cations, Divalent; D003300:Copper / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006493:Heparin / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D008077:Lipoproteins, LDL / Q000187:drug effects* / Q000378:metabolism; D010084:Oxidation-Reduction; D013997:Time Factors
D000975:Antioxidants; D002413:Cations, Divalent; D008077:Lipoproteins, LDL; D003300:Copper; D006493:Heparin