Monoclonal antibodies to Coxiella burnetii that cross-react with strain Nine Mile.
Results are presented to show the binding properties of five monoclonal antibodies directed to Coxiella burnetii Priscilla with cross-reactions to the Nine Mile strain. The monoclonal antibodies preferentially recognize phase I epitopes by ELISA and recognize phase II epitopes by immunoblotting but do not allow differentiation between so-called chronic and acute strains of C. burnetii. The only difference in reactivity was in the staining pattern revealed after reactions with lipopolysaccharide I antigens.
Sekeyová
Z
Z
Department of Rickettsiae, Slovak Academy of Sciences, Bratislava.
Kovácová
E
E
Kazár
J
J
Toman
R
R
Olvecká
S
S
D000818:Animals; D000907:Antibodies, Bacterial / Q000737:chemistry* / Q000145:classification; D000911:Antibodies, Monoclonal / Q000737:chemistry* / Q000145:classification; D003001:Cloning, Molecular; D016997:Coxiella burnetii / Q000276:immunology*; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005455:Fluorescent Antibody Technique; D015151:Immunoblotting; D007132:Immunoglobulin Isotypes / Q000032:analysis; D051379:Mice; D008807:Mice, Inbred BALB C; D013045:Species Specificity
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D007132:Immunoglobulin Isotypes