Cloning and expression of a murine cDNA homologous to the human RCK/P54, a lymphoma-linked chromosomal translocation junction gene on 11q23.
The RCK/P54 gene was previously isolated from the chromosome translocation breakpoint region on 11q23 of a human lymphoma cell line, RC-K8, with t(11;14)(q23;q32). It was found to encode a 472-483-amino-acid (aa) polypeptide belonging to an RNA helicase/translation initiation factor family. The aim of the present investigation was the isolation and comparative sequence analysis of the mouse RCK/P54 cDNA from a BALB/c spleen cDNA library. Sequence analysis revealed an open reading frame (ORF) predicting a polypeptide of 483 aa showing 97.7% identity to human RCK/p54. Northern analysis demonstrated a 7.5-kb signal in all tissues tested and in vitro translation analysis showed a 54-kDa polypeptide. These results indicate that both mouse and human RCK/P54 are highly conserved.
Laboratory of Chemotherapy, Aichi Cancer Center Research Institute, Nagoya, Japan.
M
M
Seto
K
K
Yamamoto
T
T
Takahashi
R
R
Ueda
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002880:Chromosomes, Human, Pair 11; D003001:Cloning, Molecular; D053487:DEAD-box RNA Helicases; D004265:DNA Helicases / Q000737:chemistry; D018076:DNA, Complementary; D015870:Gene Expression; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D010448:Peptide Initiation Factors / Q000737:chemistry; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D012316:RNA Nucleotidyltransferases; D012333:RNA, Messenger / Q000235:genetics; D011994:Recombinant Proteins; D017386:Sequence Homology, Amino Acid; D014178:Translocation, Genetic
D018076:DNA, Complementary; D010448:Peptide Initiation Factors; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D011994:Recombinant Proteins; D012316:RNA Nucleotidyltransferases; C490616:Ddx6 protein, mouse; C094683:DDX6 protein, human; D004265:DNA Helicases; D053487:DEAD-box RNA Helicases