A second osmosensing signal transduction pathway in yeast. Hypotonic shock activates the PKC1 protein kinase-regulated cell integrity pathway.
Yeast cells respond to hypertonic shock by activation of a (MAP) mitogen-activated protein kinase cascade called the (HOG) high osmolarity glycerol response pathway. How yeast respond to hypotonic shock is unknown. Results of this investigation show that a second MAP kinase cascade in yeast called the protein kinase C1 (PKC1) pathway is activated by hypotonic shock. Tyrosine phosphorylation of the PKC1 pathway MAP kinase increased rapidly in cells following a shift of the external medium to lower osmolarity. The intensity of the response was proportional to the magnitude of the decrease in extracellular osmolarity. This response to hypotonic shock required upstream protein kinases of the PKC1 pathway. Increasing external osmolarity inhibited tyrosine phosphorylation of the PKC1 pathway MAP kinase, a response that was blocked by BCK1-20, a constitutively active mutant in an upstream protein kinase. These results indicate that yeast contain two osmosensing signal transduction pathways, the HOG pathway and the PKC1 pathway, that respond to hypertonic and hypotonic shock, respectively.
Department of Biochemistry and Cell Biology, Rice University, Houston, Texas 77251, USA.
K R
KR
Davenport
M
M
Sohaskey
Y
Y
Kamada
D E
DE
Levin
M C
MC
Gustin
D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000378:metabolism; D004789:Enzyme Activation; D005947:Glucose / Q000494:pharmacology; D006982:Hypertonic Solutions; D009994:Osmolar Concentration; D010766:Phosphorylation; D019000:Phosphotyrosine / Q000032:analysis; D011493:Protein Kinase C / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000502:physiology*; D012462:Saline Solution, Hypertonic; D015398:Signal Transduction
D006982:Hypertonic Solutions; D012462:Saline Solution, Hypertonic; D019000:Phosphotyrosine; D011493:Protein Kinase C; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D005947:Glucose