Comparison of ELISA and HPLC for the determination of desmosine or isodesmosine in aortic tissue elastin.
We developed a rapid and simple method for estimating tissue elastin content by measuring desmosine (D) in tissue hydrolysates by competitive ELISA. We compared the ELISA previously reported HPLC methods. When D or isodesmosine (ID) in hydrolysate of the same elastin preparation were measured by the two different methods, a good linear relationship was obtained (r = 0.854 for human aorta or r = 0.938 for rabbit aorta, respectively). The ELISA method can detect as little as 6 pmol/ml and it may be useful in monitoring elastin metabolism in patients with various connective tissue diseases.
Drug Discovery Laboratory, Hoechst Japan Limited, Saitama, Japan.
T
T
Osakabe
Y
Y
Seyama
S
S
Yamashita
D000818:Animals; D001011:Aorta / Q000737:chemistry*; D001667:Binding, Competitive; D002851:Chromatography, High Pressure Liquid / Q000592:standards; D003432:Cross-Linking Reagents / Q000032:analysis; D003895:Desmosine / Q000032:analysis* / Q000378:metabolism; D004549:Elastin / Q000737:chemistry* / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay / Q000592:standards; D005260:Female; D007524:Isodesmosine / Q000032:analysis* / Q000378:metabolism; D011817:Rabbits; D015203:Reproducibility of Results
D003432:Cross-Linking Reagents; D003895:Desmosine; D004549:Elastin; D007524:Isodesmosine