Radiation-induced neoplastic transformation of human prostate epithelial cells.
We report the malignant transformation of human prostate epithelial cells (267B1) after multiple exposures to ionizing radiation. Carcinogenic progression of cells from immortal growth to anchorage-independent growth in soft agar to tumorigenicity in athymic mice resulted after a cumulative X-ray dose of 30 Gy. The tumors were characterized histologically as poorly differentiated adenocarcinomas, expressed prostate-specific antigen, and stained positive for keratin. No p53 or ras mutations were observed. Numerous chromosomal defects were noted on karyotypes after radiation exposure. However, chromosome 3 and 8 translocations were observed predominantly in the tumor outgrowths. These findings provide the first evidence of malignant transformation of human prostate epithelial cells exposed to ionizing radiation.
Department of Radiation Medicine, Vincent T. Lombardi Cancer Center, Georgetown University School of Medicine, Washington, DC 20007, USA.
M R
MR
Kuettel
P J
PJ
Thraves
M
M
Jung
S P
SP
Varghese
S C
SC
Prasad
J S
JS
Rhim
A
A
Dritschilo
D000818:Animals; D002471:Cell Transformation, Neoplastic / Q000528:radiation effects*; D002478:Cells, Cultured; D004848:Epithelium / Q000378:metabolism / Q000473:pathology / Q000528:radiation effects; D006801:Humans; D007621:Karyotyping; D007633:Keratins / Q000096:biosynthesis; D008297:Male; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D011467:Prostate / Q000378:metabolism / Q000473:pathology* / Q000528:radiation effects; D017430:Prostate-Specific Antigen / Q000096:biosynthesis
D007633:Keratins; D017430:Prostate-Specific Antigen