Stability of YACs containing ribosomal or RCP/GCP locus DNA in wild-type S. cerevisiae and RAD mutant strains.
About 2% of human YAC clones, including tandemly repeated segments color vision pigment DNA, ribosomal DNA and alphoid DNA have been reported to be inherently unstable in yeast hosts, producing more stable deletion products. YACs containing color vision red pigment gene DNA or 1.5 rDNA tandem repeat units were transformed into hosts bearing lesions at the RAD1, RAD6, RAD51, or RAD52 loci. YACs susceptible to deletion during outgrowth of wild-type cells (or in preliminary experiments, in RAD6 transformants) were stable for up to 100 generations or more in the other strains. Thus both the RAD1 and RAD51/RAD52 epistatic pathways are apparently involved in the instability of YACs containing tandem repeat loci, presumably during recombination-based deletion formation; and a yeast host disarmed in these pathways will likely maintain YACs intact that are otherwise unstable.
Department of Molecular and Cellular Biology for Pharmaceutical Sciences, Kyoto Pharmaceutical University, Japan.
K
K
Kohno
M
M
Wada
D
D
Schlessinger
M
M
D'Urso
S
S
Tanabe
T
T
Oshiro
F
F
Imamoto
D000818:Animals; D001483:Base Sequence; D018244:Chromosomes, Artificial, Yeast; D006224:Cricetinae; D045643:DNA Repair Enzymes; D004271:DNA, Fungal / Q000235:genetics* / Q000378:metabolism; D004274:DNA, Recombinant / Q000235:genetics / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000502:physiology*; D004720:Endonucleases / Q000235:genetics / Q000502:physiology*; D005136:Eye Proteins / Q000235:genetics*; D005656:Fungal Proteins / Q000235:genetics / Q000502:physiology*; D006801:Humans; D006822:Hybrid Cells; D008025:Ligases / Q000235:genetics / Q000502:physiology*; D008969:Molecular Sequence Data; D051135:Rad51 Recombinase; D051721:Rad52 DNA Repair and Recombination Protein; D011995:Recombination, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D012168:Retinal Pigments / Q000235:genetics*; D017299:Rod Opsins; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D017384:Sequence Deletion; D044763:Ubiquitin-Conjugating Enzymes
D004271:DNA, Fungal; D004274:DNA, Recombinant; D004268:DNA-Binding Proteins; D005136:Eye Proteins; D005656:Fungal Proteins; C497197:RAD52 protein, S cerevisiae; D051721:Rad52 DNA Repair and Recombination Protein; D012168:Retinal Pigments; D017299:Rod Opsins; D029701:Saccharomyces cerevisiae Proteins; C095855:long-wavelength opsin; C095854:middle-wavelength opsin; C471606:RAD6 protein, S cerevisiae; D044763:Ubiquitin-Conjugating Enzymes; C495151:RAD51 protein, S cerevisiae; C495153:RAD51 protein, human; D051135:Rad51 Recombinase; D004720:Endonucleases; C069266:RAD1 protein, S cerevisiae; D008025:Ligases; D045643:DNA Repair Enzymes