RAD58 (XRS4)--a new gene in the RAD52 epistasis group.
The RAD58 (XRS4) gene of Saccharomyces cerevisiae has been previously identified as a DNA repair gene. In this communication, we show that RAD58 also encodes an essential meiotic function. The spore inviability of rad58 strains is not rescued by a spo13 mutation. The rad50 mutation suppresses spore inviability of a spo13 rad58 strain suggesting that RAD58 acts after RAD50 in meiotic recombination. The rad58-4 mutation does not prevent mitotic recombination events. Haploid rad58 cells fail to carry out G2-repair of gamma-induced lesions, whereas rad58/rad58 diploids are able to perform some diploid-specific repair of these lesions.
Department of Molecular and Radiation Biophysics, Petersburg Nuclear Physics Institute Academy of Sciences of Russia, Gatchina, Russia.
O V
OV
Chepurnaya
S A
SA
Kozhin
V T
VT
Peshekhonov
V G
VG
Korolev
D004249:DNA Damage; D004260:DNA Repair; D004271:DNA, Fungal / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects; D004843:Epistasis, Genetic; D005656:Fungal Proteins / Q000235:genetics* / Q000502:physiology; D005720:Gamma Rays; D005800:Genes, Fungal; D005816:Genetic Complementation Test; D008540:Meiosis; D010957:Plasmids / Q000235:genetics; D011003:Ploidies; D011995:Recombination, Genetic; D012098:Reproduction; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins
D004271:DNA, Fungal; D005656:Fungal Proteins; C097192:SPO13 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins