Characterisation of an enterotoxin-negative, cytotoxin-positive strain of Clostridium sordellii.
In ileal loop assay, ELISA and anion-exchange column chromatography, Clostridium sordellii strain 6018 was shown to produce a cytotoxin, but no detectable enterotoxin. DNA sequence and polymerase chain reaction analyses indicated that the lack of enterotoxin activity is not due to a lack of gene transcription, but to lack of a major portion of the enterotoxin gene. This is the first characterisation of such a strain.
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Laboratoire de Toxinologie Bactérienne, Faculté de Médecine, Université Louis Pasteur, Strasbourg, France.
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Girardot
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D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002852:Chromatography, Ion Exchange; D003001:Cloning, Molecular; D016360:Clostridioides difficile / Q000235:genetics / Q000378:metabolism; D003013:Clostridium / Q000235:genetics* / Q000378:metabolism*; D003603:Cytotoxins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics / Q000633:toxicity; D017931:DNA Primers / Q000737:chemistry; D004269:DNA, Bacterial / Q000032:analysis / Q000737:chemistry; D004768:Enterotoxins / Q000096:biosynthesis / Q000235:genetics* / Q000633:toxicity; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007082:Ileum / Q000187:drug effects; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D016133:Polymerase Chain Reaction; D011817:Rabbits; D012689:Sequence Homology, Nucleic Acid
D003603:Cytotoxins; D017931:DNA Primers; D004269:DNA, Bacterial; D004768:Enterotoxins