Synthesis and NMR of RNA with selective isotopic enrichment in the bases.
Efficient syntheses of pyrimidine and purine nucleosides and nucleotides with selective 13C enrichment in the base moieties are described. Uridine and cytidine are labeled at position C6 and adenosine and guanosine are labeled at position C8. The selectively labeled nucleosides were converted to nucleoside triphosphates and used with in vitro transcription to synthesize labeled RNA. Isotope-edited 12C and 13C sub-spectra of a omega 1-1/2-X-filtered NOESY experiment are demonstrated to be useful for making resonance assignments and for deriving structural information in large (> 20 nt) RNA molecules. The labeled RNAs also allow heteronuclear J-couplings and relaxation parameters to be measured without complications from 13C-13C J-couplings.
Department of Chemistry, Lawrence Berkeley Laboratory, University of California, Berkeley 94720, USA.
J
J
SantaLucia
L X
LX
Shen
Z
Z
Cai
H
H
Lewis
I
I
Tinoco
D000225:Adenine / Q000138:chemical synthesis; D003562:Cytidine / Q000138:chemical synthesis; D006151:Guanosine / Q000138:chemical synthesis; D009682:Magnetic Resonance Spectroscopy; D011684:Purine Nucleosides / Q000138:chemical synthesis*; D011741:Pyrimidine Nucleosides / Q000138:chemical synthesis*; D012313:RNA / Q000138:chemical synthesis* / Q000737:chemistry; D014498:Uracil / Q000138:chemical synthesis; D014529:Uridine / Q000138:chemical synthesis
D011684:Purine Nucleosides; D011741:Pyrimidine Nucleosides; D006151:Guanosine; D014498:Uracil; D003562:Cytidine; D012313:RNA; D000225:Adenine; D014529:Uridine