The effect of an anti-glucocorticoid (ZK 98299) on thymus evolution and on hydrocortisone-induced thymolysis, intestinal brush-border enzymes and their desialylation in suckling rats.
1. The action in Onapristone infant male rats displays short-term and delayed effects. 2. Suppression of intestinal brush-border enzymes and increase of thymus mass were observed only immediately after 3-day treatment with Onapristone. After an additional 3 days its effect disappeared. There was no immediate or delayed effect of Onapristone on the desialylation of brush-border enzymes. 3. In the short-term and delayed effects, Onapristone suppressed the HC-provoked induction of several intestinal brush-border enzymes, especially alpha-glycosidases. In the delayed effect the drug also suppressed thymolysis induced by the exogeneously given glucocorticoid, and suppressed the HC-induced desialylation of a brush-border enzyme DP IV, which serves as a marker of the desialylation process. 4. These experiments seem to support a conclusion that the postnatal development of intestinal brush-border enzymes and the development of thymus in infant rats are controlled by endogeneously secreted glucocorticoids. 5. The control of sialylation of intestinal brush-border proteins by endogeneously secreted glucocorticoids during the postnatal development of the rat remains debatable.
Kraml
J
J
First Faculty of Medicine, Charles University, Prague, Czech Republic.
Kolínská
J
J
Kadlecová
L
L
Zákostelecká
M
M
Hirsová
D
D
Schreiber
V
V
D000681:Amylases / Q000378:metabolism; D000818:Animals; D000833:Animals, Suckling; D018819:Dipeptidyl Peptidase 4 / Q000378:metabolism; D004790:Enzyme Induction / Q000187:drug effects; D005087:Glucan 1,4-alpha-Glucosidase / Q000378:metabolism; D006031:Glycosylation / Q000187:drug effects; D006067:Gonanes / Q000494:pharmacology*; D006727:Hormone Antagonists / Q000494:pharmacology*; D006854:Hydrocortisone / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D007421:Intestine, Small / Q000187:drug effects* / Q000201:enzymology / Q000254:growth & development / Q000648:ultrastructure; D043322:Lactase; D008297:Male; D008871:Microvilli / Q000187:drug effects / Q000201:enzymology; D019158:N-Acetylneuraminic Acid; D009929:Organ Size / Q000187:drug effects; D011499:Protein Processing, Post-Translational / Q000187:drug effects; D051381:Rats; D012794:Sialic Acids / Q000378:metabolism*; D013393:Sucrase / Q000378:metabolism; D013950:Thymus Gland / Q000187:drug effects* / Q000254:growth & development; D001616:beta-Galactosidase / Q000378:metabolism
D006067:Gonanes; D006727:Hormone Antagonists; D012794:Sialic Acids; D000681:Amylases; D043322:Lactase; D001616:beta-Galactosidase; D005087:Glucan 1,4-alpha-Glucosidase; D013393:Sucrase; D018819:Dipeptidyl Peptidase 4; D019158:N-Acetylneuraminic Acid; C053238:onapristone; D006854:Hydrocortisone