Sequencing peptides without scanning the reflectron: post-source decay with a curved-field reflectron time-of-flight mass spectrometer.
A time-of-flight mass spectrometer has been developed using a reflectron in which the voltages placed on the lens elements correspond to a function describing the arc of a circle. This curved-field reflectron enables acquisition of focused product-ion mass spectra at a single reflectron voltage. In this paper, we present results from this instrument for the amino acid sequencing of several peptides and describe the method of mass calibration.
Middle Atlantic Mass Spectrometry Laboratory, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
M M
MM
Cordero
T J
TJ
Cornish
R J
RJ
Cotter
I A
IA
Lys
D000595:Amino Acid Sequence; D000804:Angiotensin II / Q000032:analysis; D002138:Calibration; D002364:Caseins / Q000032:analysis; D008969:Molecular Sequence Data; D010455:Peptides / Q000032:analysis*; D011199:Potentiometry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000295:instrumentation; D013373:Substance P / Q000032:analysis
D002364:Caseins; D010455:Peptides; D000804:Angiotensin II; D013373:Substance P