[Effect of benzquercin on the connective tissue of lathyritic mice. Optic and electron microscopic study].
Vascular pathology is characterized by important alterations of some vessel macromolecular constituents, such as fibrous proteins, collagens and elastin. The purpose of our study was to establish the activity of benzquercin treatment on such alterations of the vascular wall. As experimental model we used lathyrism induced in mice by chronic administration of beta-amino-propionitril (beta-APN). This compound prevents crosslink-formation in elastin and collagen and provokes a disorganization of the structure and an alteration of the physiological functions of the vascular wall. The connective tissue of the skin is also impaired simultaneously with that of the blood vessels. We compared by optical and transmission electron microscopy the morphological structure of the aorta and the skin of 3 groups of mice: a normal control group, an other which only received the beta-APN alone and a third one which received the beta-APN and the benzquercin treatment. The second group, injected with beta-APN without treatment, showed important alterations of the structure of the aorta as well as of the skin. Both fibrous proteins, collagen and elastin were concerned by these alterations, the consequence of which was an increase of the permeability of the aorta wall demonstrated with the horse-radish peroxydase as a tracer. The third group, injected with beta-APN and treated with the benzquercin, showed much less morphological disorders than the untreated group and the vascular permeability was also close to normal controls.(ABSTRACT TRUNCATED AT 250 WORDS)
Laboratoire de Biologie Cellulaire, Université Paris VII, Faculté des Sciences, Paris, France.
G
G
Azzi
M
M
Safars
E
E
Viljanen-Tarifa
E
E
Vörös
A M
AM
Robert
D000629:Aminopropionitrile / Q000009:adverse effects / Q000494:pharmacology*; D000818:Animals; D001013:Aorta, Thoracic / Q000187:drug effects* / Q000648:ultrastructure; D002199:Capillary Permeability / Q000187:drug effects*; D003094:Collagen / Q000032:analysis; D003238:Connective Tissue / Q000187:drug effects* / Q000648:ultrastructure; D004338:Drug Combinations; D004547:Elastic Tissue / Q000187:drug effects / Q000648:ultrastructure; D005419:Flavonoids / Q000494:pharmacology*; D007842:Lathyrism / Q000139:chemically induced; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008854:Microscopy, Electron; D012016:Reference Values; D012867:Skin / Q000187:drug effects / Q000648:ultrastructure
D004338:Drug Combinations; D005419:Flavonoids; D000629:Aminopropionitrile; C097365:benzquercin; D003094:Collagen
Action de la benzquercine1 sur le tissu conjonctif de la souris lathyritique. Etude par microscopie optique et électronique.