Improved detection of medically important fungi by immunoperoxidase staining with polyclonal antibodies.
This study was performed to identify pathological fungi of eight species [Aspergillus fumigatus, Candida albicans, Torulopsis (Candida) glabrata, Cryptococcus neoformans, Fusarium anthophilum, Rhizopus oryzae, Sporothrix schenckii and Trichosporon beigelii] in formalin-fixed, paraffin-embedded tissue sections by indirect immunoperoxidase staining. Mature albino rabbits were immunized with formalin-killed organisms. Antibodies were prepared by precipitation. Immunoperoxidase staining was applied to the paraffin-embedded tissue sections of experimentally infected mice and human autopsy and surgical specimens. Although the cell walls of each fungus stained clearly, many cross-reactivities appeared. However, it was possible to obtain specificity for the eight species by absorption and dilution of the antisera.
Department of Pathology, Shinshu University School of Medicine, Nagano, Japan.
M
M
Fukuzawa
H
H
Inaba
M
M
Hayama
N
N
Sakaguchi
K
K
Sano
M
M
Ito
M
M
Hotchi
D000818:Animals; D000906:Antibodies; D000918:Antibody Specificity; D003429:Cross Reactions; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D003904:Mitosporic Fungi / Q000302:isolation & purification*; D011817:Rabbits; D013047:Specific Pathogen-Free Organisms
D000906:Antibodies