Sequence identification of antigenic variants in plaque isolates of foot-and-mouth disease virus.
Foot-and-mouth disease virus isolates frequently contain mixtures of antigenic variants. Using synthetic mixtures of two 'pure' viruses which differed at one amino acid of the major epitope, it was found that a minor component present as 10% or less of the mixture would be undetected by nucleic acid sequencing.
Foreign Animal Disease Diagnostic Laboratory, National Veterinary Services Laboratories, Animal and Plant Health Inspection Service, Greenport, NY 11944, USA.
R F
RF
Meyer
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F
Brown
D000818:Animals; D000940:Antigenic Variation / Q000235:genetics; D000956:Antigens, Viral / Q000235:genetics*; D005537:Aphthovirus / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D010948:Viral Plaque Assay
D000956:Antigens, Viral