An improved autoradiographic coating technique for neurohistopathological stains and electron microscopy.
We describe modifications and improvements to our first report of a new nuclear emulsion coating technique for both light and electron microscopic autoradiography. Although this technique was originally designed to facilitate electron microscopic autoradiography, the methodology also allows pre-staining of plastic-embedded tissue sections prior to coating the slides with nuclear emulsion for light microscopic autoradiography. We now demonstrate that paraffin sections can be autoradiographically processed after being subjected to a combination of immunocytochemical reactions and special neuroanatomical strains. Parlodion film has been found to be more resistant to temperature changes and less prone to contamination than Formvar film. The shape of the double adhesive tape is an important aspect of the electron microscopic technique; it has been modified to minimize contamination and facilitate the removal of the grids from the glass slide. These technical adjustments facilitate the application of the nuclear emulsion and increase radionuclide specificity, thus expanding the investigative horizons of light and electron microscopic autoradiography.
Kornhauser
G V
GV
Department of Anatomy, George Washington University Medical Center, Washington, DC 20037, USA.
Krum
J M
JM
Rosenstein
J M
JM
D000818:Animals; D000831:Animals, Newborn; D001345:Autoradiography / Q000379:methods*; D001932:Brain Neoplasms / Q000473:pathology; D005910:Glioma / Q000473:pathology; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D009417:Nerve Tissue / Q000648:ultrastructure*; D016612:Paraffin Embedding; D016613:Plastic Embedding; D051381:Rats; D017208:Rats, Wistar; D013194:Staining and Labeling / Q000379:methods*; D016707:Tissue Fixation / Q000379:methods*