The effect of interleukin-1 beta or transforming growth factor-beta on radiation-impaired murine skin wound healing.
The ability of exogenous interleukin-1 beta (IL-1 beta) or transforming growth factor-beta (TGF-beta) to reverse radiation-induced defective wound healing was investigated. Mice were irradiated with 8.5 or 11 Gy to total body (TB), 12 or 16 Gy to hemibody (HB), or 20 or 26 Gy to skin alone immediately prior to surgical wounding in order to determine the effect of hematopoietic system depletion on cytokine action. A single dose of IL-1 beta or TGF-beta or vehicle control was applied to each wound. All radiation doses and types resulted in a deficit in wound healing when measured on Days 11 and 14. IL-1 beta enhanced wound tensile strength (WTS) in TB-irradiated mice, while TGF-beta enhanced WTS in HB-irradiated mice. Neither cytokine was effective at enhancing WTS in unirradiated or skin-only irradiated animals. In addition IL-1 beta and TGF-beta showed distinct differences in their effects on the kinetics of healing with time after wounding. The effects of TGF-beta appeared to be transient with compromise of the gain in WTS. The differences in the effects of these two cytokines in affecting wound healing reflect their involvement in different cellular events in the wound healing process.
Vegesna
V
V
Department of Radiation Oncology, University of California at Los Angeles 90024-1714, USA.
McBride
W H
WH
Taylor
J M
JM
Withers
H R
HR
D000818:Animals; D005260:Female; D007375:Interleukin-1 / Q000494:pharmacology*; D007700:Kinetics; D051379:Mice; D008809:Mice, Inbred C3H; D012867:Skin / Q000187:drug effects* / Q000528:radiation effects*; D016212:Transforming Growth Factor beta / Q000494:pharmacology*; D014916:Whole-Body Irradiation; D014945:Wound Healing / Q000187:drug effects* / Q000528:radiation effects*
D007375:Interleukin-1; D016212:Transforming Growth Factor beta