Carbohydrate-induced modulation of cell membrane--III. Interaction of sialic acid and mannose with hamster splenic lymphocytes: a spin label study.
The role of different carbohydrates as ligands for adhesion molecules has been extensively studied. However, the physiological changes in the splenic lymphocytes on binding these ligands have never been studied. In this paper, we report that binding of sialic acid or mannose to hamster splenic lymphocytes restricts the mobility of membrane proteins and lipids as studied by EPR spectroscopy using spin probes. Binding of mucin and heparin totally restricts the mobility probably due to crosslinking of the surface lectins. Binding of these ligands also results in an increase in the viscosity of the cytoplasm.
School of Life Sciences, Devi Ahilya Vishwavidyalaya, Vigyan Bhawan, Indore, India.
K
K
Hajela
R
R
Kayestha
Sumati
D000818:Animals; D006224:Cricetinae; D004347:Drug Interactions; D004578:Electron Spin Resonance Spectroscopy; D008214:Lymphocytes / Q000737:chemistry / Q000187:drug effects*; D008358:Mannose / Q000494:pharmacology*; D008563:Membrane Lipids / Q000737:chemistry*; D008647:Mesocricetus; D019158:N-Acetylneuraminic Acid; D012794:Sialic Acids / Q000494:pharmacology*; D013154:Spleen / Q000166:cytology*
D008563:Membrane Lipids; D012794:Sialic Acids; D019158:N-Acetylneuraminic Acid; D008358:Mannose