Stimulatory effect of saponin from Panax ginseng on immune function of lymphocytes in the elderly.
We used the saponin Rg1 extracted from Panax ginseng to study its effects on lymphocytes of 10 young and 19 elderly persons. The proliferative response of lymphocytes cocultured for 72 h with PHA and saponin was measured by using MTT method and the 3H-TdR incorporation procedure. PHA and Rg1 had stimulative effects on the phenotype of lymphocytes (P < 0.001). Rg1 also increased the fluidity of lymphocyte membrane of the aged (P < 0.001). The CD25 and CD45RA positive cells of lymphocytes in the elderly were lower than those of the young people, 8.6% +/- 2.7% vs 10.43% +/- 3.5%; 20.95% +/- 15.5% vs. 50.86% +/- 4.2%, respectively. More CD45RO positive cells (41.5% +/- 13.9%) than CD45RA positive cell lymphocyte populations were seen in the aged. The CD45RO positive cells of the young people were 39.63% +/- 3.2%. We discussed the cause of declined immune function of lymphocytes of aged person and the mechanism of the effect of P. ginseng on lymphocytes.
Liu
J
J
Department of Immunology, Beijing Institute of Geriatrics, Beijing Hospital, China.
Wang
S
S
Liu
H
H
Yang
L
L
Nan
G
G
D000368:Aged; D000375:Aging / Q000276:immunology*; D002478:Cells, Cultured / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects*; D008214:Lymphocytes / Q000187:drug effects*; D008297:Male; D005894:Panax / Q000276:immunology*; D010641:Phenotype; D010946:Plants, Medicinal; D012503:Saponins / Q000276:immunology* / Q000494:pharmacology*
D012503:Saponins