Color selection with a hygromycin-resistance-based Escherichia coli-mycobacterial shuttle vector.
Hygromycin-resistance (HyR)-based Escherichia coli-mycobacterial shuttle plasmids have high efficiencies of transformation and a broad mycobacterial host range. We have introduced a lacZ alpha (encoding the alpha-polypeptide fragment of beta-galactosidase (beta Gal))-multiple cloning site cassette into a HyR-based shuttle vector to generate a plasmid with nine unique cloning sites and the added feature of beta Gal color selection in appropriate E. coli host strains.
Department of Molecular Microbiology and Immunology, School of Hygiene and Public Health, Johns Hopkins University, Baltimore, MD 21205, USA.
N S
NS
Howard
J E
JE
Gomez
C
C
Ko
W R
WR
Bishai
D002934:Cinnamates; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000235:genetics*; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D006921:Hygromycin B / Q000031:analogs & derivatives; D009161:Mycobacterium / Q000235:genetics*; D015183:Restriction Mapping
D002934:Cinnamates; D006921:Hygromycin B; C026273:hygromycin A