Up-regulation of IL-2 receptor alpha and MHC class II expression on lymphocyte subpopulations from bovine leukemia virus infected lymphocytotic cows.
Infection with bovine leukemia virus (BLV) leads to a persistent lymphocytosis (PL) characterized by a marked increase in circulating B lymphocytes that express the orthologue of CD5. To gain insight into the factors accounting for lymphocytosis, experiments were conducted to determine the functional activation status of lymphocytes from BLV seronegative and BLV infected aleukemic cows with PL. Stimulation with the B lymphocyte mitogen Staphylococcus aureus Cowan strain I (SAC), recombinant human interleukin-2 (rIL-2), or pokeweed mitogen (PWM), a T lymphocyte-dependent B lymphocyte mitogen, revealed differences in the pattern of expression of IL-2 receptor alpha (IL-2R alpha) and major histocompatibility (MHC) class II molecules on B and T lymphocytes from uninfected and BLV infected PL cows. rIL-2 induced expression of IL-R alpha on B lymphocytes from PL cows but not B lymphocytes from BLV seronegative cows. SAC alone, or in combination with rIL-2, had no effect on B lymphocytes from BLV seronegative cows. However, rIL-2 alone or in combination with SAC induced expression of IL-2R alpha on B lymphocytes from PL cows. PWM stimulated expression of IL-2R alpha on bovine B lymphocytes regardless of BLV status, and induced a significantly higher level of expression on B lymphocytes from PL cows. Mitogens and rIL-2 had a similar stimulatory effect on induction of IL-2R alpha expression on CD4 T lymphocytes regardless of BLV status. Only PWM induced expression of IL-2R alpha on bovine CD8 T lymphocytes and induced a significantly higher level of expression on this T lymphocyte subset from PL cows. Examination of freshly isolated B lymphocytes from PL cows revealed increased spontaneous expression of the MHC class II molecule compared to B lymphocytes from control cows. None of the culture conditions examined induced MHC-II expression on CD4 and CD8 T lymphocytes from BLV seronegative cows. In contrast, SAC+IL-2 and PWM induced MHC-II expression on CD4 and CD8 T lymphocytes from BLV infected PL cows, resulting in a significantly greater proportions of these lymphocyte subsets expressing this molecule compared to CD4 and CD8 T lymphocytes from control cows. The data indicate that infection with BLV affects the response of B and T lymphocytes to signals of activation, up-regulating the expression of surface molecules involved in both direct contact and cytokine-mediated T lymphocyte-dependent B lymphocyte activation.
Department of Veterinary Microbiology and Pathology, Washington State University, Pullman 99164-7040, USA.
D M
DM
Stone
A J
AJ
Hof
W C
WC
Davis
D000818:Animals; D001402:B-Lymphocytes / Q000378:metabolism; D002417:Cattle; D016583:Enzootic Bovine Leukosis / Q000276:immunology*; D005260:Female; D000949:Histocompatibility Antigens Class II / Q000096:biosynthesis*; D016130:Immunophenotyping; D001909:Leukemia Virus, Bovine / Q000276:immunology* / Q000302:isolation & purification; D016131:Lymphocyte Subsets / Q000145:classification / Q000276:immunology* / Q000378:metabolism*; D015375:Receptors, Interleukin-2 / Q000096:biosynthesis*; D013601:T-Lymphocytes / Q000378:metabolism; D015854:Up-Regulation / Q000276:immunology*
D000949:Histocompatibility Antigens Class II; D015375:Receptors, Interleukin-2