Antigenicity of the rabbit hemorrhagic disease virus studied by its reactivity with monoclonal antibodies.
A panel of anti-rabbit hemorrhagic disease virus (anti-RHDV) monoclonal antibodies was produced and characterized. The ability of the MAbs to recognize epitopes present on RHDV capsids, European brown hare syndrome virus capsids and RHDV subunits was determined. Preliminary results on the neutralizing capacity of the MAbs were obtained by in vivo protection experiments. The antigenic map of RHDV obtained by this study is consistent with the current models of the calicivirus structure.
Department of Virology, Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia, Brescia, Italy.
L
L
Capucci
G
G
Frigoli
L
L
Rønshold
A
A
Lavazza
E
E
Brocchi
C
C
Rossi
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000914:Antibodies, Viral / Q000276:immunology*; D017250:Caliciviridae Infections / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D018604:Epitope Mapping; D006385:Hemagglutination Inhibition Tests; D017929:Hemorrhagic Disease Virus, Rabbit / Q000276:immunology*; D011485:Protein Binding; D011817:Rabbits; D015678:Viral Structural Proteins / Q000378:metabolism; D014765:Viral Vaccines / Q000276:immunology
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D015678:Viral Structural Proteins; D014765:Viral Vaccines; C081149:viral protein 60, rabbit hemorrhagic disease virus