An open reading frame in the approximately 28-kb tox-argk gene cluster encodes a polypeptide with homology to fatty acid desaturases.
Part of an apparent open reading frame in the tox-argK gene cluster of Pseudomonas syringae pathovar phaseolicola (Psp) potentially encodes a polypeptide with sequence similarity to fatty acid desaturases (DES). Escherichia coli B expressing this segment under T7 promoter control produced a 34-kDa polypeptide. The possible involvement of a DES in facilitating phaseolotoxin (Ptx) secretion at the low temperatures normally required for its synthesis and the evolutionary implications about the origin of the tox-argK gene cluster are discussed.
Hatziloukas
E
E
Department of Environmental Science, Policy and Management, University of California, Berkeley 94720, USA.
Panopoulos
N J
NJ
Delis
S
S
Prosen
D E
DE
Schaad
N W
NW
D000595:Amino Acid Sequence; D001483:Base Sequence; D017931:DNA Primers / Q000737:chemistry; D004269:DNA, Bacterial / Q000235:genetics; D005098:Exotoxins / Q000378:metabolism; D044943:Fatty Acid Desaturases / Q000235:genetics; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D005810:Multigene Family; D016366:Open Reading Frames; D009952:Ornithine / Q000031:analogs & derivatives; D010935:Plant Diseases / Q000382:microbiology; D011549:Pseudomonas / Q000235:genetics*; D015183:Restriction Mapping; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D012995:Solubility
D017931:DNA Primers; D004269:DNA, Bacterial; D005098:Exotoxins; C021234:phaseolotoxin; D009952:Ornithine; D044943:Fatty Acid Desaturases