Influence of the estrus cycle on the discrimination of apparent neuroactive steroid site subtypes on the gamma-aminobutyric acidA receptor complex in the rat.
Estrus cycle-related changes in gamma-aminobutyric acid (GABA)A receptor complex (GRC) sensitivity to modulation by reduced progesterone metabolites is suggestive of a possible mechanism for maintaining brain homeostasis in the presence of fluctuating levels of these neuroactive metabolites. In addition, certain endogenously occurring pregnanediols are selective for apparent neuroactive steroid site subtypes discriminated by the progesterone metabolite 3 alpha-hydroxy-5 beta-pregnan-20-one (3 alpha,5 beta-P) on the GRC. Thus, it was of interest to evaluate the influence of gender and the estrus cycle on the ability of 3 alpha,5 beta-P and its 20-reduced analog 5 beta-pregnan-3 alpha,20 beta-diol to differentiate neuroactive steroid site subtypes. Neuroactive steroid modulation of [35S]t-butylbicyclophosphorothionate ([35S]TBPS) binding to the GRC in rats during estrus, diestrus and after ovariectomy (OVX) was measured in washed cortical P2 homogenates in the presence or absence of exogenous GABA. During diestrus, the inability of 5 beta-pregnan-3 alpha,20 beta-diol to allosterically modulate [35S]TBPS binding in the absence of GABA coincides with the inability of 3 alpha,5 beta-P to modulate [35S]TBPS binding with high potency. In contrast, the addition of GABA to the assay produced high potency inhibition of [35S]TBPS binding by each steroid. Remarkably, although findings in diestrus and OVX homogenates were no different from those observed in males, the proportions and IC50 values of the two sites discriminated by 3 alpha,5 beta-P in [35S]TBPS binding assays during the estrus phase were significantly different from male, OVX and diestrus rats.(ABSTRACT TRUNCATED AT 250 WORDS)
McCauley
L D
LD
Department of Pharmacology, College of Medicine, University of California, Irvine, USA.
Gee
K W
KW
D000494:Allosteric Regulation; D000818:Animals; D001665:Binding Sites; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000378:metabolism; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism; D004971:Estrus / Q000502:physiology*; D005260:Female; D008297:Male; D011280:Pregnanolone / Q000378:metabolism* / Q000494:pharmacology; D011374:Progesterone / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011963:Receptors, GABA-A / Q000378:metabolism*; D012727:Sex Characteristics; D013462:Sulfur Radioisotopes
D019086:Bridged Bicyclo Compounds, Heterocyclic; D011963:Receptors, GABA-A; D013462:Sulfur Radioisotopes; D011374:Progesterone; C037476:tert-butylbicyclophosphorothionate; D011280:Pregnanolone