Determination of Ras and GTPase-activating protein interactions by kinetic competition assay.
Schaber
M D
MD
Department of Cancer Research, Merck Research Laboratories, West Point, Pennsylvania 19486, USA.
Gibbs
J B
JB
D000595:Amino Acid Sequence; D000818:Animals; D001667:Binding, Competitive; D004926:Escherichia coli / Q000235:genetics; D020690:GTPase-Activating Proteins; D006160:Guanosine Triphosphate / Q000378:metabolism; D006868:Hydrolysis; D066298:In Vitro Techniques; D007700:Kinetics; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology; D017354:Point Mutation; D011485:Protein Binding; D011506:Proteins / Q000032:analysis / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D020703:ras GTPase-Activating Proteins; D018631:ras Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*
D020690:GTPase-Activating Proteins; D010446:Peptide Fragments; D011506:Proteins; D011994:Recombinant Proteins; D020703:ras GTPase-Activating Proteins; D006160:Guanosine Triphosphate; D018631:ras Proteins