[Analyses of T cell clonality in mice with autoimmune sialoadenitis].
T cells are believed to be deeply involved in the pathogenesis of organ-specific autoimmune diseases. However, it remains unknown whether antigen-specific immune responses occur at the inflammatory sites in vivo. As a model of human Sjögren's syndrome, we investigated the T cell clonality of mice with autoimmune sialoadenitis, i.e. IQI/Jcl-(Saegusa et al), thymus grafted (TG) nude-(Taguchi et al), and MRL/lpr-SCID (Hayashi et al) mice; using RT-PCR-SSCP method. As a result, accumulations of distinct T cell clones were demonstrated in the salivary and lacrimal glands in these mice, without V beta restriction. Moreover, a part of the accumulating clones were commonly detected among multiple glands, suggesting the existence of a common and specific immune response probably toward autoantigen(s) expressed on the affected glands.
Institute of Medical Science, St. Marianna University.
K
K
Masuko
D000818:Animals; D002999:Clone Cells; D004195:Disease Models, Animal; D006801:Humans; D007765:Lacrimal Apparatus / Q000276:immunology; D051379:Mice; D016133:Polymerase Chain Reaction; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000276:immunology; D012469:Salivary Glands / Q000276:immunology; D012859:Sjogren's Syndrome / Q000209:etiology*; D013601:T-Lymphocytes / Q000276:immunology*
D016693:Receptors, Antigen, T-Cell, alpha-beta