Definition of lipid membrane structural parameters from neutronographic experiments with the help of the strip function model.
Neutron diffraction is an effective method for investigating model and biological membranes. Yet, to obtain accurate structural information it is necessary to use deuterium labels and much time is needed to acquire experimental data as there are a large number of diffraction reflections to register. This paper offers a way to define the hydrophobic boundary position in lipid membranes with high accuracy and for this purpose it is sufficient to take into consideration three structural factors. The method is based on modeling the density of the neutron diffraction amplitude rho(x) in the direction of the bilayer plane normal by means of a strip function, but it also takes into consideration the fact that the multiplication of the strip function amplitude rho i by the step width zi-zi-1 makes the sum of neutron scattering amplitudes of the atoms included in the step region. On the basis of the analysis of a large number of experimental data for different membranes, the effectiveness of this method in the determination of the position of hydrophilic/hydrophobic boundary is demonstrated, including the case of various rho(x) modifications in the region of polar heads and also the different phase states of membranes. However, it is shown in the present paper that the strip function model is not an adequate instrument for the determination of other structural parameters of membranes.
Gordeliy
V I
VI
Frank Laboratory of Neutron Physics, Joint Institute for Nuclear Research, Dubna, Moscow District, Russia.
Kiselev
M A
MA
D015060:1,2-Dipalmitoylphosphatidylcholine; D008051:Lipid Bilayers / Q000737:chemistry*; D008433:Mathematics; D008954:Models, Biological; D008958:Models, Molecular; D008962:Models, Theoretical; D009502:Neutrons; D010713:Phosphatidylcholines / Q000737:chemistry*; D010742:Phospholipid Ethers; D013329:Structure-Activity Relationship
D008051:Lipid Bilayers; D010713:Phosphatidylcholines; D010742:Phospholipid Ethers; D015060:1,2-Dipalmitoylphosphatidylcholine; C028144:palmitoyl-palmityl-lecithin ether; C017251:1,2-oleoylphosphatidylcholine