Early ultrastructural changes of antral mucosa with aspirin in the absence of Helicobacter pylori.
OBJECTIVE
To describe the ultrastructural changes that occur in human antral mucosa following direct application of aspirin in volunteers without Helicobacter pylori infection.


METHODS
Ten healthy male volunteers without H pylori infection underwent three consecutive endoscopies (at zero, one and five hours). At the first endoscopy, two biopsy specimens were obtained (one for histology and the other for electron microscopy (EM)). At subsequent endoscopies, a single biopsy specimen was obtained for EM. A 50 ml solution of aspirin (concentration 3 mg/ml) was applied to the antral mucosa at the first endoscopy in five subjects; the other five subjects received 50 ml distilled water (placebo).


RESULTS
The ultrastructural appearance of the first biopsy specimen in all subjects and subsequent biopsy specimens in the placebo treated subjects was normal. The aspirin treated group had evidence of intercellular oedema, widening of capillary fenestrae, rupturing of apical membranes, and dilatation of endoplasmic reticulum and mitochondria after one hour; these changes were more marked at five hours. Tight junctions were maintained.


CONCLUSIONS
This is the first study to describe the early ultrastructural changes in antral mucosa induced by aspirin in subjects without H pylori infection.
Department of Gastroenterology, Meath Hospital, Dublin, Ireland.
C J
CJ
McCarthy
E
E
Sweeney
C
C
O'Morain
D000328:Adult; D001241:Aspirin / Q000494:pharmacology*; D005753:Gastric Mucosa / Q000187:drug effects* / Q000648:ultrastructure; D005773:Gastroscopy; D016480:Helicobacter pylori; D006801:Humans; D008297:Male; D008854:Microscopy, Electron; D011706:Pyloric Antrum / Q000187:drug effects / Q000648:ultrastructure; D013997:Time Factors
D001241:Aspirin