Sensitivity and specificity of an isoelectric focusing fraction of Blastomyces dermatitidis yeast lysate antigen for the detection of canine blastomycosis.
Blastomyces dermatitidis yeast lysate antigen (T-58, dog isolate) fractions prepared using the Rotofor preparative isoelectric focusing (IEF) cell (Bio-Rad) were compared with B. dermatitidis yeast lysate and filtrate reagents with respect to the detection of antibodies in sera from dogs with blastomycosis, histoplasmosis, coccidioidomycosis, cryptococcosis and aspergillosis. A horseradish peroxidase enzyme immunoassay with Turbo TMB substrate was used in the study. One particular IEF fraction (pH 4.3) was optimal in the assay, and it exhibited greater sensitivity (100%) and specificity (93%) than the lysate or filtrate preparations. The highest degree of cross-reactivity was encountered with the histoplasmosis and coccidioidomycosis specimens and considerably less with the cryptococcosis and aspergillosis sera. Studies are in progress to purify further the optimal IEF fraction.
Department of Biological Sciences, Idaho State University, Pocatello, USA.
M A
MA
Fisher
J L
JL
Bono
R O
RO
Abuodeh
A M
AM
Legendre
G M
GM
Scalarone
D000818:Animals; D000908:Antibodies, Fungal / Q000097:blood* / Q000276:immunology; D000946:Antigens, Fungal / Q000276:immunology / Q000302:isolation & purification; D001228:Aspergillosis / Q000662:veterinary; D001759:Blastomycosis / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary*; D003047:Coccidioidomycosis / Q000662:veterinary; D003429:Cross Reactions; D003453:Cryptococcosis / Q000662:veterinary; D004283:Dog Diseases / Q000175:diagnosis*; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006660:Histoplasmosis / Q000662:veterinary; D007525:Isoelectric Focusing; D009024:Morphogenesis; D012680:Sensitivity and Specificity; D015003:Yeasts / Q000276:immunology
D000908:Antibodies, Fungal; D000946:Antigens, Fungal