DNA separation with field inversion capillary electrophoresis.
We have built an automated pulsed field capillary electrophoresis system on the basis of a commercially available device. Using entangled polymer solutions as separating matrix, we demonstrate a considerable improvement of separation of double-stranded DNA in the range of 1-50 kbp under pulsed field conditions. The influence of the main parameters, i.e. pulse frequency and electric field, is studied and the results are compared to existing electrophoresis theories.
Laboratoire de Physique des Surfaces et Interfaces (URA CNRS 1379), Institut Curie, Paris, France.
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C
Heller
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Pakleza
J L
JL
Viovy
D001322:Autoanalysis; D017122:Bacteriophage T4 / Q000235:genetics; D010582:Bacteriophage lambda / Q000235:genetics; D010584:Bacteriophage phi X 174 / Q000235:genetics; D004279:DNA, Viral / Q000302:isolation & purification*; D004560:Electricity; D004586:Electrophoresis; D019075:Electrophoresis, Capillary / Q000379:methods* / Q000706:statistics & numerical data; D008956:Models, Chemical; D011108:Polymers; D012996:Solutions; D013997:Time Factors
D004279:DNA, Viral; D011108:Polymers; D012996:Solutions