Molecular cloning of the human gene SUVCC3 associated with the formation of DNA-protein crosslinks following exposure to solar UV radiation.
DRP 153 cells, which are hypersensitive to solar UV and deficient in the formation of DNA-protein crosslinks (DPC) following irradiation, were transfected with human DNA and a secondary transformant obtained in which a normal DPC response and solar UV sensitivity reestablished. DNA from this secondary transformant was used to construct a genomic DNA library from which a recombinant phage was isolated containing the human gene capable of restoring a normal DPC response and solar UV sensitivity to DRP 153. This gene has been designated SUVCC3 to denote solar UV cross-complementing gene number 3.
Department of Radiation Oncology, Mount Sinai School of Medicine, City University of New York, New York 10029, USA.
B S
BS
Rosenstein
C A
CA
Vaslet
D000328:Adult; D000818:Animals; D001973:Bromodeoxyuridine; D002460:Cell Line; D002470:Cell Survival / Q000528:radiation effects; D003001:Cloning, Molecular; D003432:Cross-Linking Reagents; D004247:DNA / Q000378:metabolism / Q000528:radiation effects*; D004260:DNA Repair; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects; D006801:Humans; D007700:Kinetics; D008027:Light; D010782:Photolysis; D011506:Proteins / Q000378:metabolism / Q000528:radiation effects*; D011898:Ranidae; D012867:Skin / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects; D013472:Sunlight; D013997:Time Factors; D014162:Transfection; D014466:Ultraviolet Rays
D003432:Cross-Linking Reagents; D011506:Proteins; D004247:DNA; D001973:Bromodeoxyuridine