Cloning and sequence analysis of the Corynebacterium diphtheriae dtxR homologue from Streptomyces lividans and S. pilosus encoding a putative iron repressor protein.
The iron-regulated promoter involved in desferrioxamine B synthesis of Streptomyces pilosus contains a region homologous to the iron repressor (DtxR)-binding site of the diphtheria toxin gene promoter in Corynebacterium diphtheriae [Günter et al., J. Bacteriol. 175 (1993) 3295-3302]. Here, we report the cloning and sequencing of the putative Streptomyces iron repressor gene, homologous to dtxR of C. diphtheriae. The N-terminal 139 amino acids of the deduced protein are 73% identical to DtxR.
Animal Health Biotechnology, Ciba-Geigy AG, Basel, Switzerland.
K
K
Günter-Seeboth
T
T
Schupp
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular; D003353:Corynebacterium diphtheriae / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics; D005798:Genes, Bacterial; D007501:Iron / Q000378:metabolism*; D008969:Molecular Sequence Data; D012097:Repressor Proteins / Q000235:genetics*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013302:Streptomyces / Q000235:genetics*
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; C075586:DtxR protein, Corynebacterium diphtheriae; D012097:Repressor Proteins; D007501:Iron