Removal of human immunodeficiency virus by an 0.04-micron membrane filter.
We tested the ability of a 0.04-micron nylon membrane filter to remove human immunodeficiency virus (HIV) from tissue culture media containing 10% fetal calf serum. Endpoint titrations of infectious virus (ID50) were performed on lymphocyte cultures. The presence of virus in the cultures was determined using an enzyme-linked immunoabsorbant assay (ELISA) of the HIV-1 P24 core antigen. In repeated experiments, filtration of the virus suspension resulted in the removal of HIV below detectable limits. The titer reduction was estimated to be greater than 8.5 x 10(2). These results suggest that this filter is effective in removing HIV from fluids containing serum or serum products.
Biological Products Branch, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA.
K H
KH
Oshima
T W
TW
Comans
A K
AK
Highsmith
E W
EW
Ades
D003470:Culture Media; D004797:Enzyme-Linked Immunosorbent Assay; D005374:Filtration / Q000379:methods; D016655:HIV Core Protein p24 / Q000032:analysis; D015497:HIV-1 / Q000302:isolation & purification*; D006801:Humans; D008214:Lymphocytes / Q000821:virology; D008567:Membranes, Artificial; D008851:Micropore Filters; D009757:Nylons; D013601:T-Lymphocytes / Q000821:virology
D003470:Culture Media; D016655:HIV Core Protein p24; D008567:Membranes, Artificial; D009757:Nylons