[The use of the immunoperoxidase test with monoclonal antibodies for detecting influenza A viruses].
The immunoperoxidase test was used to detect influenza virus in cells of a chorionallanthois shell of infected chicken embryos. Application of monoclonal antibodies D8 and A11 in the analysis has permitted detecting reproduction of type A (subtypes H1N1, H2N2, H3N2) viruses, the PGA titre of the respective allantois liquids being not lower than 1:16. The matrix protein and hemagglutinin, detection of which underlies this analysis, were found on the cell membrane, in the perinucleus region and as cytoplasmic inclusions.
Bakova
A A
AA
Gusev
V A
VA
Popov
I A
IA
Gordienko
A I
AI
Krivorutchenko
Iu L
IuL
Krivoshein
Iu S
IuS
D000482:Allantois / Q000276:immunology / Q000821:virology; D000818:Animals; D000911:Antibodies, Monoclonal; D000956:Antigens, Viral / Q000032:analysis; D002642:Chick Embryo; D002823:Chorion / Q000276:immunology / Q000821:virology; D006389:Hemagglutinins, Viral / Q000276:immunology; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007124:Immunoenzyme Techniques; D009980:Influenza A virus / Q000276:immunology*; D014763:Viral Matrix Proteins / Q000276:immunology
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D006389:Hemagglutinins, Viral; C031540:M-protein, influenza virus; D014763:Viral Matrix Proteins
Ispol'zovanie immunoperoksidaznogo testa s monoklonal'nymi antitelami dlia vyiavleniia virusov grippa A.