Calpain activity of hypertrophic hearts from hypertensive rats.
Heart tissue contains large amounts of the Ca(2+)-activated proteinase calpain which has been assigned a specific function in the turnover of muscle protein. The objective of the present study was to determine calpain (E.C. 3.4.22.17)-like activity in homogenates of left ventricle from hypertensive rats that developed ventricular hypertrophy. Calpain activity was assayed using heat-denatured azocasein as a substrate in the presence of 1 mM calcium and corrected by subtraction of the Ca(2+)-independent activities. The latter were measured in the presence of 1 mM EGTA and the products read at 440 nm. Male Wistar rats (225 g) were assigned to control (N = 8, normal drinking water), salt (N = 6, drinking water containing 1% NaCl) and DOCA-salt (N = 6, deoxycorticosterone acetate, 8 mg/kg, sc, twice a week for 20 days plus drinking water containing 1% NaCl) groups. SHR (N = 6, spontaneously hypertensive rats) were also used. The calpain activity of the control group was at 3.90 +/- 0.22 mU/g wet weight tissue. Hypertension induced significant left ventricular hypertrophy in DOCA-salt rats (26%) and in SHR (54%) and a 30% decrease in calpain activity in both groups (P < 0.01). In the high salt load (salt group) calpain activity was also decreased, but this was not accompanied by hypertrophy. In the present indirect measurement of protein degradation capacity of heart tissue homogenates the proteolytic activity was activated (221%) by 1 mM calcium and inhibited (84%) by 1 mM EGTA after a 48-h incubation period, indicating the destruction of the calpain inhibitor.(ABSTRACT TRUNCATED AT 250 WORDS)
Departamento de Ciências Fisiológicas, Universidade Federal do Espírito Santo, Vitória, Brasil.
M A
MA
Cicilini
M M
MM
Resende
N S
NS
Bissoli
E C
EC
Vasquez
A M
AM
Cabral
D000818:Animals; D002154:Calpain / Q000378:metabolism*; D003900:Desoxycorticosterone / Q000008:administration & dosage; D017379:Hypertrophy, Left Ventricular / Q000378:metabolism*; D008297:Male; D011489:Protein Denaturation / Q000187:drug effects; D051381:Rats; D011918:Rats, Inbred SHR; D017208:Rats, Wistar; D014020:Tissue Extracts / Q000502:physiology
D014020:Tissue Extracts; D003900:Desoxycorticosterone; D002154:Calpain