Construction and characterization of a NotI linking library from human chromosome region 1q25-qter.
Chromosome 1q25-qter-specific NotI linking clones have been isolated from a NotI linking library that was constructed using DNA from MCH206.1 somatic cell hybrid cells. These cells contain chromosome 1q25-qter translocated to human chromosome Xp22 as the only human genetic material in mouse background. Sixty-eight NotI linking clones have been mapped by a combination of fluorescence in situ hybridization and R-banding to cytogenetic bands on the long arm of chromosome 1. The relative order of 11 NotI clones and their relation to known chromosome 1 markers have also been determined in 1q32 and 1q41, where the genes of Van der Woude and Usher syndrome type IIa have been previously mapped: cen-chr1.14-chr1.79-chr1.56-chr1.11-chr1.9 5- chr1.58 (chr1.74)-D1S70-chr1.15-chr1.82 (chr1.143)-chr1.62-D1S81-tel. The 1q32- and 1q41-specific NotI linking clones were sequenced in the vicinity of the NotI site. They were analyzed in terms of nucleotide composition, G+C content, frequency of CpG dinucleotides, and protein coding potentials. Most of the 1q32-q41-specific NotI linking clones were derived from CpG islands. Sequences of three NotI linking clones proved to be identical with known genes. Six of the remaining eight had a high potential for coding regions and shared short homologous regions with sequences in the GenBank database. The NotI linking clones and the identified CpG islands will provide valuable resources for constructing a long-range restriction map of chromosome 1q25-q44 and for the eventual isolation of disease genes of Van der Woude syndrome (1q32-q41) and Usher syndrome type IIa (1q41).
Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha 68198-5660, USA.
C B
CB
Talmadge
D K
DK
Zhen
J Y
JY
Wang
P
P
Berglund
B F
BF
Li
M D
MD
Weston
W J
WJ
Kimberling
E R
ER
Zabarovsky
E J
EJ
Stanbridge
G
G
Klein
D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002871:Chromosome Banding; D002874:Chromosome Mapping; D002878:Chromosomes, Human, Pair 1; D015342:DNA Probes; D015252:Deoxyribonucleases, Type II Site-Specific; D015226:Dinucleoside Phosphates / Q000032:analysis; D015723:Gene Library; D008040:Genetic Linkage; D006801:Humans; D006822:Hybrid Cells; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction; D012091:Repetitive Sequences, Nucleic Acid; D014178:Translocation, Genetic; D014960:X Chromosome
D015342:DNA Probes; D015252:Deoxyribonucleases, Type II Site-Specific; D015226:Dinucleoside Phosphates; D015345:Oligonucleotide Probes; C015772:CpG dinucleotide; C038565:GCGGCCGC-specific type II deoxyribonucleases