Molecular characterization of two forms of nontoxic-nonhemagglutinin components of Clostridium botulinum type A progenitor toxins.
The entire sequences of the type A nontoxic-nonhemagglutinin gene and an adjacent open reading frame designated as orf 22-a, which are located between the neurotoxin and the HA-35 genes were determined. SDS-PAGE and N-terminal amino acid sequence analyses of the purified type A progenitor toxins (12S, 16S and 19S) indicate that the nontoxic-nonhemagglutinins of 16S and 19S are single peptides of approximately 120k, but that of 12S has a cleavage at the site between Pro-144 and Phe-145 of this protein.
Department of Bacteriology, Okayama University Medical School, Japan.
R
R
Fujita
Y
Y
Fujinaga
K
K
Inoue
H
H
Nakajima
H
H
Kumon
K
K
Oguma
D000595:Amino Acid Sequence; D001483:Base Sequence; D001905:Botulinum Toxins / Q000737:chemistry* / Q000145:classification / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D006384:Hemagglutination; D008969:Molecular Sequence Data; D009498:Neurotoxins / Q000737:chemistry / Q000235:genetics; D016366:Open Reading Frames / Q000235:genetics; D011498:Protein Precursors / Q000737:chemistry; D016415:Sequence Alignment
D009498:Neurotoxins; D011498:Protein Precursors; D001905:Botulinum Toxins