Leukocyte-derived neutrophil chemotactic factor-2 produced by infiltrated leukocytes in allergic inflammation model in rats is macrophage inflammatory protein-2.
In the air pouch-type allergic inflammation model in rats, leukocytes collected from the pouch fluid 4 h after the antigen challenge produced proteinaceous chemotactic factors for neutrophils. The leukocytes from the immunized rats produced significantly higher amount of the chemotactic factors than that from the non-immunized rats. The major chemotactic factor, leukocyte-derived neutrophil chemotactic factor (LDNCF)-2, was purified and found to be identical with rat macrophage inflammatory protein (MIP)-2 by N-terminal amino acid sequence analysis. Expression of MIP-2 mRNA was higher in the leukocytes from the immunized rats than that from the non-immunized rats. Possible roles of LDNCF-2 (MIP-2) in neutrophil infiltration in the allergic inflammation is discussed.
Department of Pathophysiological Biochemistry, Faculty of Pharmaceutical Sciences, Tohoku University, Miyagi, Japan.
J
J
Tanabe
M
M
Watanabe
S
S
Mue
K
K
Ohuchi
D000595:Amino Acid Sequence; D000818:Animals; D000941:Antigens / Q000008:administration & dosage / Q000276:immunology / Q000633:toxicity*; D001483:Base Sequence; D054426:Chemokine CXCL2; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D017077:Culture Media, Conditioned / Q000737:chemistry; D016207:Cytokines / Q000096:biosynthesis* / Q000494:pharmacology; D017449:Dermatitis, Allergic Contact / Q000276:immunology / Q000378:metabolism*; D007114:Immunization; D007249:Inflammation / Q000139:chemically induced / Q000276:immunology / Q000378:metabolism*; D007279:Injections, Subcutaneous; D007525:Isoelectric Focusing; D007962:Leukocytes / Q000276:immunology / Q000378:metabolism*; D008297:Male; D008969:Molecular Sequence Data; D015846:Monokines / Q000096:biosynthesis* / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects; D016133:Polymerase Chain Reaction; D051381:Rats; D017207:Rats, Sprague-Dawley; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D012710:Serum Albumin, Bovine / Q000008:administration & dosage / Q000276:immunology / Q000633:toxicity*; D010132:p-Azobenzenearsonate / Q000008:administration & dosage / Q000276:immunology / Q000633:toxicity
D000941:Antigens; D054426:Chemokine CXCL2; D017077:Culture Media, Conditioned; D016207:Cytokines; D015846:Monokines; C076853:azobenzenearsonate-acetyl bovine serum albumin conjugate; D012710:Serum Albumin, Bovine; D010132:p-Azobenzenearsonate