Long-term normalization of GLUT 4 protein content in skeletal muscle of streptozotocin-diabetic Lewis rats after islet transplantation.
Islet transplantation under the kidney capsule of STZ-diabetic Lewis rats was able to maintain near-normoglycemia over a period of 6 months. Fasting insulin in these animals was higher compared to controls but did not increase after feeding. Plasma glucose following an OGTT at 2 months was only slightly impaired, and after 6 months was more severely impaired in the Tx rats. An IVGTT 6 months after Tx confirmed impaired glucose tolerance and showed a loss of first phase insulin release. GLUT 4 protein content in skeletal muscle was completely restored in Tx animals. In conclusion, long-term near-normoglycemia after syngeneic islet transplantation under the kidney capsule of STZ-diabetic Lewis rats is associated with complete normalization of skeletal muscle GLUT 4 protein content, even in the presence of abnormal glucose tolerance and impaired insulin secretion.
Joslin Diabetes Center, Harvard Medical School, Boston, Massachusetts, USA.
R
R
Weitgasser
A M
AM
Davalli
R
R
Napoli
J V
JV
Capotorto
D T
DT
Finegood
S
S
Bonner-Weir
G C
GC
Weir
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism / Q000601:surgery*; D051275:Glucose Transporter Type 4; D016381:Islets of Langerhans Transplantation / Q000502:physiology; D008297:Male; D009004:Monosaccharide Transport Proteins / Q000378:metabolism*; D009124:Muscle Proteins; D018482:Muscle, Skeletal / Q000378:metabolism*; D051381:Rats; D011917:Rats, Inbred Lew; D013997:Time Factors
D001786:Blood Glucose; D051275:Glucose Transporter Type 4; D009004:Monosaccharide Transport Proteins; D009124:Muscle Proteins; C495694:Slc2a4 protein, rat