Presence of beta-citryl-L-glutamic acid in the lens: its possible role in the differentiation of lens epithelial cells into fiber cells.
The beta-CG concentration in the chicken brain was high during embryonic development and decreased rapidly to a lower level close to hatching, while the concentration in the eyeball which was also high during the embryonic life retained a fairly high level after hatching. The distribution of beta-CG in the bovine eye was determined. About 95% of total beta-CG content in the whole eye was localized in the lens. However, the distribution of beta-CG in the eye varied depending on species. beta-CG was exclusively localized in the lens in the eyes of fish and mammals, but distributed in both lens and retina in frogs. The molecule was localized in the retina rather than the lens in the chicken eye, although the concentrations was extremely low compared to those in the mammalian, amphibian and fish eyes. It was found that beta-CG is present ubiquitously in the lens or retina in various species. The distribution of beta-CG in the bovine lens was determined in the three cortex regions and nucleus. beta-CG was present at the highest concentration in the equatorial cortex, at a moderate concentration in the posterior and anterior cortex, and at the lowest concentration in the nucleus. Similar distribution patterns were also found in the rabbit and rat lens. When embryonic chick lens epithelial cells were cultured in the presence of fetal calf serum, the cells elongated, differentiated into fiber cells and formed lentoid bodies. The cells of lentoid bodies were stained strongly by the anti-beta-CG antibody, while cells around the structures were not. In addition, the beta-CG content in the lenses from the galactose cataractous rat decreased to about 20-30% of that in the normal lens. These findings suggest that beta-CG may play a role in the differentiation of epithelial cells into fiber cells.
Faculty of Pharmaceutical Sciences, Kobe-Gakuin University, Japan.
M
M
Tsumori
M
M
Asakura
M
M
Narahara
T
T
Ogawa
M
M
Nakae
S
S
Nakagawa
Y
Y
Kawai
H
H
Morino
T
T
Hama
M
M
Miyake
D000818:Animals; D001921:Brain / Q000196:embryology / Q000378:metabolism; D002386:Cataract / Q000378:metabolism; D002417:Cattle; D002454:Cell Differentiation; D002478:Cells, Cultured; D002645:Chickens; D049832:Decapodiformes; D004847:Epithelial Cells; D005123:Eye / Q000737:chemistry / Q000196:embryology; D005399:Fishes; D005971:Glutamates / Q000032:analysis / Q000378:metabolism* / Q000502:physiology; D007904:Lens Cortex, Crystalline / Q000737:chemistry; D007908:Lens, Crystalline / Q000737:chemistry* / Q000166:cytology*; D008297:Male; D051379:Mice; D011817:Rabbits; D011892:Rana catesbeiana; D051381:Rats; D013045:Species Specificity
D005971:Glutamates; C018491:beta-citrylglutamic acid