High-performance liquid chromatographic determination of 4,4'-dihydroxybenzophenone-2,4-dinitrophenylhydrazone in plasma.
An analytical method has been developed for the determination of 4,4'-dihydroxybenzophenone-2,4-dinitrophenylhydrazone (I, trade name A-007) in plasma. Plasma samples are primed with the internal standard, 2,2'-dihydroxybenzophenone-2,4-dinitrophenylhydrazone (II), deproteinized with acetonitrile, centrifuged and filtered prior to assay. The components are then separated on a reversed-phase column with retention times of 4.4 and 6.0 min for I and II, respectively. Ultraviolet detection at 365 nm was employed and little interference with the analyte or the internal standard was noted from other plasma components. This method has been applied to the plasma of rats and monkeys doses for pharmacokinetic and toxicity studies.
Rodgers
A H
AH
DEKK-TEC Incorporated, New Orleans, LA 70119, USA.
Subramanian
S
S
Morgan
L R
LR
D000818:Animals; D000970:Antineoplastic Agents / Q000097:blood*; D002138:Calibration; D002522:Chlorocebus aethiops; D002851:Chromatography, High Pressure Liquid; D006835:Hydrazones / Q000097:blood*; D010636:Phenols / Q000097:blood*; D051381:Rats; D017207:Rats, Sprague-Dawley
C097674:4,4'-dihydroxybenzophenone-2,4-dinitrophenylhydrazone; D000970:Antineoplastic Agents; D006835:Hydrazones; D010636:Phenols