Effects of myosin light chain kinase inhibitors on delayed rectifier potassium current in bullfrog sympathetic neurons.
Actions of myosin light chain kinase inhibitors were tested on delayed rectifier potassium current (IK) in dissociated bullfrog sympathetic neurons. A microbial product, wortmannin (10 microM, extracellularly) and a synthetic peptide, SM-1 (20 microM, intracellularly) caused approximately 35 mV hyperpolarizing shift of the inactivation curve. Substitution of ATP (1.15 mM) in the pipette solution with 5'-adenylylimidodiphosphate mimicked the actions of wortmannin and SM-1. Results suggest that phosphorylation of myosin may modulate kinetics for the inactivation of IK.
Tokimasa
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Department of Physiology, Kurume University School of Medicine, Japan.
D000266:Adenylyl Imidodiphosphate / Q000494:pharmacology; D000730:Androstadienes / Q000494:pharmacology; D000818:Animals; D004594:Electrophysiology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005728:Ganglia, Sympathetic / Q000166:cytology / Q000187:drug effects; D066298:In Vitro Techniques; D009219:Myosin-Light-Chain Kinase / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D010446:Peptide Fragments / Q000494:pharmacology; D015221:Potassium Channels / Q000187:drug effects / Q000378:metabolism*; D011892:Rana catesbeiana; D013564:Sympathetic Nervous System / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D000077191:Wortmannin
D000730:Androstadienes; D004791:Enzyme Inhibitors; D010446:Peptide Fragments; D015221:Potassium Channels; C090954:SM 1 peptide; D000266:Adenylyl Imidodiphosphate; D009219:Myosin-Light-Chain Kinase; D000077191:Wortmannin