Effects of nocodazole and taxol on glycine evoked currents on rat spinal cord neurones in culture.
We examined the effect of altering the cytoskeleton polymerization state by treatment with nocodazole and taxol on glycine-evoked currents in patch-clamp recordings from cultured spinal cord neurones. Adding ATP and GTP to the pipette solution did not prevent the rundown of the peak current. In the absence or in the presence of ATP, the proportion of the non-desensitizing part of the glycine evoked-current declined with time. Adding intracellular GTP and ATP stabilized glycine-evoked responses although the proportion of non-inactivating current was reduced. Nocodazole reduced by itself the proportion of the non-inactivating current whereas taxol (with ATP and GTP) had an opposite effect. These results suggest that the polymerization state of microtubules has functional consequences on glycine receptors.
INSERM U261, Département des Biotechnologies, Institut Pasteur, Paris, France.
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Delon
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Legendre
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D001704:Biopolymers; D002478:Cells, Cultured; D018118:Chloride Channels / Q000187:drug effects; D005071:Evoked Potentials / Q000187:drug effects; D006160:Guanosine Triphosphate / Q000494:pharmacology; D008870:Microtubules / Q000187:drug effects*; D009474:Neurons / Q000187:drug effects*; D015739:Nocodazole / Q000494:pharmacology*; D017239:Paclitaxel / Q000494:pharmacology*; D018408:Patch-Clamp Techniques; D051381:Rats; D018009:Receptors, Glycine / Q000187:drug effects*; D013116:Spinal Cord / Q000166:cytology / Q000187:drug effects*
D001704:Biopolymers; D018118:Chloride Channels; D018009:Receptors, Glycine; D006160:Guanosine Triphosphate; D000255:Adenosine Triphosphate; D017239:Paclitaxel; D015739:Nocodazole