Ectopic expression of a bacterial lacZ gene in the limbic system of transgenic mice.
In three independent lines of transgenic mice, a 3.6 kb 5'-flanking sequence of the uroplakin II gene consistently drives the ectopic expression of a bacterial lacZ reporter gene in brain, in addition to its specific expression in the suprabasal layers of the urothelium. The ectopic expression in brain is especially noteworthy insofar as it is confined to structures comprising the limbic system. These findings provide additional evidence that the cells forming such functional systems share specific biochemical properties, and also indicate that this promoter may be useful as a tool for studying the effects of overexpression of proteins in anatomically and functionally defined central nervous system pathways.
Department of Pharmacology, University of New York Health Science Center, Brooklyn 11203, USA.
B
B
Meyer-Puttlitz
J H
JH
Lin
T T
TT
Sun
R K
RK
Margolis
D000818:Animals; D001419:Bacteria; D005696:Galactosidases / Q000235:genetics; D015870:Gene Expression / Q000235:genetics*; D007763:Lac Operon / Q000235:genetics*; D008032:Limbic System / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics; D051379:Mice; D008822:Mice, Transgenic; D011401:Promoter Regions, Genetic; D060170:Uroplakin II
D008565:Membrane Proteins; C554659:Upk2 protein, mouse; D060170:Uroplakin II; D005696:Galactosidases