Endothelial barrier function and Na+/K(+)-ATPase pump density in herpetic stromal disease.
OBJECTIVE
Corneal edema is a significant component of the various forms of herpes simplex virus type 1 (HSV-1)-induced stromal disease. Maintenance of corneal thickness, a reflection of corneal hydration, depends on a physical barrier formed by endothelial cell-cell junctions and by the activity of Na+/K(+)-ATPase pumps that regulate ion flux and thus influence water movement through this cell layer. These functions were measured in corneas with increased corneal thickness caused by HSV-1-induced stromal disease to determine their contribution to the pathogenesis of the edema.


METHODS
Stromal disease with corneal edema was induced in rabbits by intrastromal injection of the RE strain of HSV-1. At various times after infection, during the development of and recovery from stromal disease, endothelial barrier function and Na+/K(+)-ATPase pump sites were measured in excised rabbit corneas.


RESULTS
The endothelial permeability coefficient, Ktrans, for 14C-dextran, 3H-inulin, and 14C-mannitol, were not altered significantly during periods of maximal corneal edema and stromal disease. Endothelial Na+/K(+)-ATPase pump density, as measured by ouabain binding, showed a statistically significant (P < 0.05) decrease in HSV-1-infected corneas during peak edema compared to mock antigen-injected or uninjected control corneas. Pump density returned to baseline values by 24 days after infection, concurrent with the resolution of corneal edema.


CONCLUSIONS
These results indicate that corneal endothelial barrier function was not altered in this form of HSV-1-induced stromal edema; however, pump density was reduced significantly.
O'Brien
W J
WJ
Department of Ophthalmology, Medical College of Wisconsin, Milwaukee 53226, USA.
Palmer
M L
ML
Guy
J
J
Taylor
J L
JL
D000818:Animals; D001665:Binding Sites; D001693:Biological Transport, Active / Q000502:physiology; D002452:Cell Count; D002463:Cell Membrane Permeability; D015715:Corneal Edema / Q000209:etiology / Q000378:metabolism / Q000821:virology; D003319:Corneal Stroma / Q000821:virology*; D004728:Endothelium, Corneal / Q000166:cytology / Q000502:physiology*; D004791:Enzyme Inhibitors / Q000378:metabolism; D018259:Herpesvirus 1, Human; D016849:Keratitis, Herpetic / Q000209:etiology / Q000378:metabolism* / Q000821:virology; D008297:Male; D010042:Ouabain / Q000378:metabolism; D011817:Rabbits; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*; D013047:Specific Pathogen-Free Organisms
D004791:Enzyme Inhibitors; D010042:Ouabain; D000254:Sodium-Potassium-Exchanging ATPase