Cell wall sorting of lipoproteins in Staphylococcus aureus.
Many surface proteins are thought to be anchored to the cell wall of gram-positive organisms via their C termini, while the N-terminal domains of these molecules are displayed on the bacterial surface. Cell wall anchoring of surface proteins in Staphylococcus aureus requires both an N-terminal leader peptide and a C-terminal cell wall sorting signal. By fusing the cell wall sorting of protein A to the C terminus of staphylococcal beta-lactamase, we demonstrate here that lipoproteins can also be anchored to the cell wall of S. aureus. The topology of cell wall-anchored beta-lactamase is reminiscent of that described for Braun's murein lipoprotein in that the N terminus of the polypeptide chain is membrane anchored whereas the C-terminal end is tethered to the bacterial cell wall.
Department of Microbiology and Immunology, UCLA School of Medicine 90024, USA.
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D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D002473:Cell Wall / Q000737:chemistry; D010450:Endopeptidases / Q000378:metabolism; D004768:Enterotoxins / Q000737:chemistry; D008074:Lipoproteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D008248:Lysostaphin / Q000378:metabolism; D008565:Membrane Proteins; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009113:Muramidase / Q000378:metabolism; D010455:Peptides; D010457:Peptidoglycan / Q000378:metabolism; D011480:Protease Inhibitors / Q000494:pharmacology; D011498:Protein Precursors / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D021382:Protein Sorting Signals / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000378:metabolism; D012697:Serine Endopeptidases; D013205:Staphylococcal Protein A / Q000737:chemistry / Q000235:genetics; D013211:Staphylococcus aureus / Q000737:chemistry* / Q000378:metabolism; D001618:beta-Lactamases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D004768:Enterotoxins; D008074:Lipoproteins; D008565:Membrane Proteins; D010455:Peptides; D010457:Peptidoglycan; D011480:Protease Inhibitors; D011498:Protein Precursors; D021382:Protein Sorting Signals; D011993:Recombinant Fusion Proteins; D013205:Staphylococcal Protein A; C031818:enterotoxin B, staphylococcal; C017339:globomycin; D009113:Muramidase; D010450:Endopeptidases; D012697:Serine Endopeptidases; C026515:type I signal peptidase; D008248:Lysostaphin; D001618:beta-Lactamases