NADP-malic enzyme from maize leaves: a fluorescence study.
NADP-malic enzyme from maize leaves is covalently labeled with a fluorescent-SH reactive probe eosin-5-maleimide (EMA), which reacts with groups that are totally protected by NADP against inactivation. The comparison of the emission fluorescence spectra of the native and the modified enzyme suggests the proximity of the fluorescent groups of the native enzyme (probably tryptophanyl groups) and the EMA modified residues. Intrinsic fluorescence quenching studies shows that NADP is the only substrate capable to interact with the fluorescent excited groups of the enzyme, while Mg2+ is able to increase this interaction. Quenching studies of EMA-bound fluorescence shows that the NADP-binding site was modified and thus uncapable of further interaction with the nucleotide. When the results of protection studies are combined with those of extrinsic quenching experiments, we must conclude that EMA reacts with sulfhydryl groups that are involved in the NADP-binding site of the enzyme.
Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI), (CONICET, Fund M Lillo, Universidad Nacional de Rosario), Argentina.
M F
MF
Drincovich
C S
CS
Andreo
D001665:Binding Sites; D004801:Eosine Yellowish-(YS) / Q000031:analogs & derivatives; D005456:Fluorescent Dyes; D008291:Malate Dehydrogenase / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D009249:NADP / Q000378:metabolism; D018515:Plant Leaves; D011487:Protein Conformation; D013050:Spectrometry, Fluorescence; D013379:Substrate Specificity; D003313:Zea mays / Q000201:enzymology*
D005456:Fluorescent Dyes; D009249:NADP; C033531:eosin maleimide; D008291:Malate Dehydrogenase; C031917:malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+); D004801:Eosine Yellowish-(YS)