The XRCC4 gene encodes a novel protein involved in DNA double-strand break repair and V(D)J recombination.
The XR-1 Chinese hamster ovary cell line is impaired in DNA double-strand break repair (DSBR) and in ability to support V(D)J recombination of transiently introduced substrates. We now show that XR-1 cells support recombination-activating gene 1- and 2-mediated initiation of V(D)J recombination within a chromosomally integrated substrate, but are highly impaired in ability to complete the process by forming coding and recognition sequence joins. On this basis, we isolated a human cDNA sequence, termed XRCC4, whose expression confers normal V(D)J recombination ability and significant restoration of DSBR activity to XR-1, clearly demonstrating that this gene product is involved in both processes. The XRCC4 gene maps to the previously identified locus on human chromosome 5, is deleted in XR-1 cells, and encodes a ubiquitously expressed product unrelated to any described protein.
Center for Blood Research, Harvard University Medical School, Boston, Massachusetts 02115, USA.
Z
Z
Li
T
T
Otevrel
Y
Y
Gao
H L
HL
Cheng
B
B
Seed
T D
TD
Stamato
G E
GE
Taccioli
F W
FW
Alt
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D016466:CHO Cells / Q000502:physiology; D006224:Cricetinae; D004247:DNA / Q000235:genetics; D004260:DNA Repair / Q000235:genetics* / Q000528:radiation effects; D018076:DNA, Complementary / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D015870:Gene Expression / Q000235:genetics; D015723:Gene Library; D005816:Genetic Complementation Test; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D011995:Recombination, Genetic / Q000235:genetics*
D018076:DNA, Complementary; D004268:DNA-Binding Proteins; C101467:XRCC4 protein, human; D004247:DNA