Spermine action on mitochondrial H(+)-ATPase activity and proliferation rate of Trypanosoma cruzi.
Activity of H(+)-ATPase of Trypanosoma cruzi (RA strain) submitochondrial particles is increased in parasites which have a low spermine content caused by growing in a polyamine free medium or in a medium containing inhibitors of polyamine synthesis. Under these conditions, the proliferation rate is markedly decreased. Kinetics of the enzyme inhibition by spermine indicates a non-competitive inhibition. Spermine, through its action on the H(+)-ATPase hydrophobic environment, affects the enzyme activity. Together with the ATPase protein inhibitor, this could be a mechanism regulating ATP levels needed for the parasite proliferation.
Departamento de Bioquímica, Facultad de Medicina, Universidad de Buenos Aires, Argentina.
M N
MN
Schwarcz de Tarlovsky
M C
MC
Rilo
S M
SM
Hernandez
A M
AM
Bedoya
E M
EM
Lammel
E L
EL
Isola
D000818:Animals; D003513:Cycloheximide / Q000494:pharmacology; D000518:Eflornithine / Q000494:pharmacology; D007700:Kinetics; D008928:Mitochondria / Q000201:enzymology*; D006180:Proton-Translocating ATPases / Q000187:drug effects / Q000378:metabolism*; D011700:Putrescine / Q000378:metabolism; D013095:Spermidine / Q000378:metabolism; D013096:Spermine / Q000378:metabolism / Q000494:pharmacology*; D013367:Submitochondrial Particles / Q000201:enzymology; D014349:Trypanosoma cruzi / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development*
D013096:Spermine; D003513:Cycloheximide; D006180:Proton-Translocating ATPases; D013095:Spermidine; D011700:Putrescine; D000518:Eflornithine