Autoregulation of iclR, the gene encoding the repressor of the glyoxylate bypass operon.
The aceBAK operon was partially induced by a multicopy plasmid which carried the promoter region of the gene which encodes its repressor, iclR. Gel shift and DNase I analyses demonstrated that IclR binds to its own promoter. Disruption of iclR increased the expression of an iclR::lacZ operon fusion. Although aceBAK and iclR are both regulated by IclR, aceBAK expression responds to the carbon source, while expression of iclR does not.
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Department of Biochemistry, University of Minnesota, Minneapolis 55455, USA.
Sunnarborg
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LaPorte
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D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D001667:Binding, Competitive; D004269:DNA, Bacterial / Q000378:metabolism; D029968:Escherichia coli Proteins; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics*; D005798:Genes, Bacterial / Q000235:genetics; D006038:Glyoxylates / Q000378:metabolism*; D007522:Isocitrate Lyase / Q000235:genetics; D008292:Malate Synthase / Q000235:genetics; D008969:Molecular Sequence Data; D009876:Operon / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics; D017346:Protein Serine-Threonine Kinases / Q000235:genetics; D011993:Recombinant Fusion Proteins; D012097:Repressor Proteins / Q000235:genetics* / Q000378:metabolism; D017384:Sequence Deletion; D014157:Transcription Factors; D014158:Transcription, Genetic / Q000235:genetics; D001616:beta-Galactosidase / Q000096:biosynthesis / Q000235:genetics
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; D006038:Glyoxylates; D011993:Recombinant Fusion Proteins; D012097:Repressor Proteins; D014157:Transcription Factors; C067479:IclR protein, E coli; D008292:Malate Synthase; D017346:Protein Serine-Threonine Kinases; C042301:isocitrate dehydrogenase kinase; D001616:beta-Galactosidase; D007522:Isocitrate Lyase; C031150:glyoxylic acid