Free calcium in Micrasterias: local gradients are not detected in growing lobes.
Intracellular free Ca2+ ([Ca2+]) has been measured in growing unicells of two species of the green alga, Micrasterias, which have been injected with the indicator dye fura-2-dextran. Ratiometric imaging of Micrasterias denticulata yields levels of 170 to 200 nM [Ca2+] but fails to reveal a significant [Ca2+] gradient associated with the tips of growing lobes, or in any other region of the cell. In Micrasterias muricata slight elevations from a basal value of 350 to 500 nM have been observed, but these might be due to a general inward leakage of Ca2+ at the plasma membrane which is enhanced at the narrow lobes of this cell because of their greater relative surface to volume ratio. Experimental perturbation of the intracellular [Ca2+] with injection of the ion or the addition of the non-fluorescent ionophore, Br-A23187, reveal that [Ca2+] elevations can be generated and indicate that if they naturally occurred, the image system would have detected them. Further evidence that [Ca2+] gradients are lacking derives from studies with BAPTA-type buffers. Injection of 5,5'-dibromo BAPTA and 4,4'-difluoro BAPTA, which in several other systems are the most effective at dissipating intracellular [Ca2+] gradients, have no effect on development of Micrasterias. Taken together, these studies indicate that lobe outgrowth in Micrasterias does not occur in association with marked localized [Ca2+] gradients.
Holzinger
A
A
Institute for Plant Physiology, University of Salzburg, Austria.
Callaham
D A
DA
Hepler
P K
PK
Meindl
U
U
D002118:Calcium / Q000378:metabolism*; D002455:Cell Division; D000460:Chlorophyta / Q000254:growth & development / Q000378:metabolism*; D004533:Egtazic Acid / Q000031:analogs & derivatives / Q000494:pharmacology
D004533:Egtazic Acid; C025603:1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid; D002118:Calcium