Sequencing and linkage analysis of a Coxiella burnetii 2.1 kb NotI fragment.
Most of the Coxiella burnetii isolates (72 of 80) available in our institute share a 2.1 kb NotI fragment. Sequence analysis revealed two incomplete open reading frames (ORF) for putative genes hemA and bcR coding for glutamyl-tRNA-reductase and bicyclomycin resistance protein, respectively. Upon completing the ORF for the hemA gene by sequencing the adjacent NotI/EcoRI fragment a third ORF for the putative prfA gene was determined coding for release factor 1, an enzyme catalysing the last step in protein biosynthesis.
Willems
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Institut für Hygiene und Infektionskrankheiten der Tiere, Justus-Liebig-Universität, Giessen, Germany.
Thiele
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Krauss
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D000445:Aldehyde Oxidoreductases / Q000235:genetics; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000494:pharmacology; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D016997:Coxiella burnetii / Q000235:genetics*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000235:genetics*; D004352:Drug Resistance, Microbial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D029968:Escherichia coli Proteins; D005798:Genes, Bacterial / Q000235:genetics; D008040:Genetic Linkage; D008969:Molecular Sequence Data; D015513:Oncogene Proteins / Q000235:genetics; D016366:Open Reading Frames / Q000235:genetics; D010454:Peptide Termination Factors / Q000235:genetics; D011505:Protein-Tyrosine Kinases; D011518:Proto-Oncogene Proteins; D051562:Proto-Oncogene Proteins c-bcr; D012486:Salmonella typhimurium / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific) / Q000235:genetics
D000900:Anti-Bacterial Agents; D019086:Bridged Bicyclo Compounds, Heterocyclic; D029968:Escherichia coli Proteins; D015513:Oncogene Proteins; D010454:Peptide Termination Factors; D011518:Proto-Oncogene Proteins; C489005:prfA protein, E coli; D000445:Aldehyde Oxidoreductases; C067282:glutamyl tRNA reductase; C020098:DNA modification methylase EcoRI; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific); D011505:Protein-Tyrosine Kinases; D051562:Proto-Oncogene Proteins c-bcr; D015252:Deoxyribonucleases, Type II Site-Specific; C038565:GCGGCCGC-specific type II deoxyribonucleases; C024504:bicozamycin