Use of a monoclonal antibody against the antigen B of Echinococcus granulosus for purification and detection of antigen B.
A monoclonal antibody directed against the 8 kDa subunit of antigen B of Echinococcus granulosus was raised. This antibody was used for purification of antigen B by affinity chromatography. SDS-PAGE and immunological analysis of the purified antigen B demonstrated that the 8 kDa antigen B subunit was purified to homogeneity. The purified antigen retained its strong immunoreactivity in ELISA using human hydatid sera. Furthermore, a sandwich ELISA was established for detection of antigen B from hydatid cysts. The usefulness of this test system was demonstrated by the detection of antigen B in human hydatid cyst fluids, thus confirming the diagnosis of an echinococcal disease.
Laboratorio di Immunologia, Istituto Superiore di Sanità, Rome, Italy.
E
E
Ortona
A
A
Siracusano
A
A
Castro
R
R
Rigano
F
F
Mühlschlegel
S
S
Ioppolo
S
S
Notargiacomo
M
M
Frosch
D000818:Animals; D000909:Antibodies, Helminth; D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth / Q000032:analysis* / Q000276:immunology / Q000302:isolation & purification; D004443:Echinococcosis / Q000175:diagnosis* / Q000469:parasitology / Q000662:veterinary; D004446:Echinococcus / Q000276:immunology* / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D015801:Helminth Proteins; D006801:Humans; D008074:Lipoproteins / Q000032:analysis* / Q000276:immunology / Q000302:isolation & purification; D051379:Mice; D008807:Mice, Inbred BALB C; D012756:Sheep; D012757:Sheep Diseases / Q000175:diagnosis
D000909:Antibodies, Helminth; D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth; D015801:Helminth Proteins; D008074:Lipoproteins; C089273:antigen B, Echinococcus