Listeriolysin is a potent inducer of the phosphatidylinositol response and lipid mediator generation in human endothelial cells.
The impact of Listeria monocytogenes listeriolysin O (LLO) secretion on phosphoinositide metabolism and mediator (platelet-activating factor and prostaglandin I2) generation was investigated in human umbilical vein endothelial cells. Wild-type L. monocytogenes, purified LLO, and an L. innocua strain engineered to secrete LLO all elicited a strong response, whereas mutant strains defective in LLO production were ineffective. Thus, human umbilical vein endothelial cell stimulation by listeriae is linked to production of LLO.
Sibelius
U
U
Department of Internal Medicine, Justus Liebig University, Giessen, Federal Republic of Germany.
Rose
F
F
Chakraborty
T
T
Darji
A
A
Wehland
J
J
Weiss
S
S
Seeger
W
W
Grimminger
F
F
D001427:Bacterial Toxins; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000378:metabolism; D011464:Epoprostenol / Q000096:biosynthesis*; D006360:Heat-Shock Proteins / Q000494:pharmacology*; D006460:Hemolysin Proteins / Q000494:pharmacology*; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D010716:Phosphatidylinositols / Q000096:biosynthesis*; D010972:Platelet Activating Factor / Q000096:biosynthesis*
D001427:Bacterial Toxins; D006360:Heat-Shock Proteins; D006460:Hemolysin Proteins; D010716:Phosphatidylinositols; D010972:Platelet Activating Factor; D011464:Epoprostenol; D007770:L-Lactate Dehydrogenase; C038705:hlyA protein, Listeria monocytogenes