Airway responsiveness to bradykinin is related to eosinophilic inflammation in asthma.
We investigated the relationship between airway inflammation and airway responsiveness, as assessed by PD15, to methacholine and to bradykinin in asthmatic patients. Bronchoalveolar lavage (BAL), bronchial biopsies, and methacholine and bradykinin challenges were performed in 18 nonsmoking subjects with mild or moderate perennial asthma. Bradykinin PD15 correlated negatively with eosinophil count in BAL (p < 0.05), in the epithelium (p < 0.05), in the lamina propria (p = 0.02) and in the total submucosa (p < 0.01). Conversely, no significant correlation existed between airway responsiveness to methacholine and eosinophil count in BAL or in airway mucosa. Airway responsiveness to either agonist did not correlate with the thickness of the basement membrane, the shedding of the airway epithelium, the count of lymphocytes in the airway mucosa, or the percentage of neutrophils, lymphocytes, and macrophage in BAL. The presence of degranulated eosinophils was associated with an increased number of eosinophils in the airway epithelium (p = 0.04), in the lamina propria (p = 0.03), in the total submucosa (p = 0.02), and with increased airway responsiveness to bradykinin (p < 0.02). We conclude that in asthmatic patients, airway responsiveness to bradykinin but not to methacholine is related to the magnitude of eosinophilic inflammation in the airway mucosa.
Service de Pneumologie, U.F.R. Cochin-Port-Royal, Université René Descartes, Paris, France.
G L
GL
Roisman
J G
JG
Lacronique
N
N
Desmazes-Dufeu
C
C
Carré
A
A
Le Cae
D J
DJ
Dusser
D000328:Adult; D000368:Aged; D001249:Asthma / Q000473:pathology*; D001706:Biopsy; D001920:Bradykinin; D001980:Bronchi / Q000473:pathology*; D001985:Bronchial Provocation Tests; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology*; D001999:Bronchoscopy; D003627:Data Interpretation, Statistical; D004804:Eosinophils; D005260:Female; D006801:Humans; D008214:Lymphocytes; D008264:Macrophages; D008297:Male; D016210:Methacholine Chloride; D008875:Middle Aged; D009504:Neutrophils
D016210:Methacholine Chloride; D001920:Bradykinin