A novel isoenzyme of ascorbate peroxidase localized on glyoxysomal and leaf peroxisomal membranes in pumpkin.
A novel isoenzyme of ascorbate peroxidase with molecular mass of 31 kDa was found to be localized on membranes of microbodies. Intact microbodies had no latent ascorbate peroxidase activity, an indication that the active site of the ascorbate peroxidase was exposed to the cytosol and the peroxidase would scavenge hydrogen peroxide leaked from microbodies.
Department of Cell Biology, National Institute for Basic Biology, Okazaki, Japan.
K
K
Yamaguchi
H
H
Mori
M
M
Nishimura
D000595:Amino Acid Sequence; D060387:Ascorbate Peroxidases; D007425:Intracellular Membranes / Q000201:enzymology; D007527:Isoenzymes / Q000378:metabolism*; D008830:Microbodies / Q000201:enzymology*; D008969:Molecular Sequence Data; D010544:Peroxidases / Q000378:metabolism*; D018515:Plant Leaves / Q000201:enzymology*; D017386:Sequence Homology, Amino Acid; D014675:Vegetables / Q000201:enzymology*
D007527:Isoenzymes; D010544:Peroxidases; D060387:Ascorbate Peroxidases