Autoregulation of iclR, the gene encoding the repressor of the glyoxylate bypass operon.
The aceBAK operon was partially induced by a multicopy plasmid which carried the promoter region of the gene which encodes its repressor, iclR. Gel shift and DNase I analyses demonstrated that IclR binds to its own promoter. Disruption of iclR increased the expression of an iclR::lacZ operon fusion. Although aceBAK and iclR are both regulated by IclR, aceBAK expression responds to the carbon source, while expression of iclR does not.
Department of Biochemistry, University of Minnesota, Minneapolis 55455, USA.
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LaPorte
D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D001667:Binding, Competitive; D004269:DNA, Bacterial / Q000378:metabolism; D029968:Escherichia coli Proteins; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics*; D005798:Genes, Bacterial / Q000235:genetics; D006038:Glyoxylates / Q000378:metabolism*; D007522:Isocitrate Lyase / Q000235:genetics; D008292:Malate Synthase / Q000235:genetics; D008969:Molecular Sequence Data; D009876:Operon / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics; D017346:Protein Serine-Threonine Kinases / Q000235:genetics; D011993:Recombinant Fusion Proteins; D012097:Repressor Proteins / Q000235:genetics* / Q000378:metabolism; D017384:Sequence Deletion; D014157:Transcription Factors; D014158:Transcription, Genetic / Q000235:genetics; D001616:beta-Galactosidase / Q000096:biosynthesis / Q000235:genetics
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; D006038:Glyoxylates; D011993:Recombinant Fusion Proteins; D012097:Repressor Proteins; D014157:Transcription Factors; C067479:IclR protein, E coli; D008292:Malate Synthase; D017346:Protein Serine-Threonine Kinases; C042301:isocitrate dehydrogenase kinase; D001616:beta-Galactosidase; D007522:Isocitrate Lyase; C031150:glyoxylic acid