Molecular analysis of mutations in thyroid tumors with TGGE.
Immunohistochemical demonstration of overexpression of the p53 protein indicates a mutational alteration of the gene. Our own investigations of 59 differentiated thyroid carcinomas revealed an overexpression in 15% of the tumors. A correlation to unfavourable tumor prognosis was found (stage I and II: 0/11 (0%); stage III: 4/26 (14%); stage IV: 5/22 (23%)). For screening of one out of more than 300 possible mutations temperature gradient gel electrophoresis was employed. Analysis of the highly-conserved regions of the p53 gene (exon 5 to 8) could demonstrate a mutation in only 1 out of 31 differentiated thyroid carcinomas. The question arises whether accumulation of the protein is due to a mutational event or rather other molecular mechanisms.
Simon
D
D
Department of Surgery, Heinrich Heine University Düsseldorf, Germany.
Goretzki
P E
PE
Schäfer
C
C
Gorelov
V
V
Ebeling
B
B
Röher
H D
HD
D018263:Adenocarcinoma, Follicular / Q000235:genetics*; D001483:Base Sequence; D018276:Carcinoma, Medullary / Q000235:genetics*; D002291:Carcinoma, Papillary / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D005091:Exons; D006801:Humans; D015335:Molecular Probes / Q000235:genetics; D008969:Molecular Sequence Data; D009154:Mutation; D016133:Polymerase Chain Reaction; D013696:Temperature; D013964:Thyroid Neoplasms / Q000235:genetics*; D016606:Thyroid Nodule / Q000235:genetics
D015335:Molecular Probes