Serum antibody responses in opisthorchiasis.
We evaluated an enzyme-linked immunosorbent assay using crude parasite homogenates as a diagnostic test for Opisthorchis viverrini infection in humans. Serum antibody (Ab) responses to O. viverrini adult worm homogenate (AWH) and metacercaria homogenate (MH) were studied in 83 infected residents of an opisthorchiasis-endemic area in Thailand. Elevated levels of Ab persisted for over 1 year following curative treatment with praziquantel, and cross-reactivity to O. viverrini AWH and MH antigens was observed in sera from individuals with other parasitic infections. Serum Ab to crude AWH and MH are therefore unsuitable for immunodiagnosis since they may be non-specific and would not differentiate between ongoing and past infection.
Department of Microbiology and Infectious Diseases, University of Calgary, Canada.
P S
PS
Akai
S
S
Pungpak
W
W
Chaicumpa
V
V
Kitikoon
Y
Y
Ruangkunaporn
D
D
Bunnag
A D
AD
Befus
D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D000917:Antibody Formation; D000918:Antibody Specificity; D000980:Antiplatyhelmintic Agents / Q000627:therapeutic use; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005500:Follow-Up Studies; D006801:Humans; D007070:Immunoglobulin A / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D009889:Opisthorchiasis / Q000175:diagnosis* / Q000188:drug therapy / Q000276:immunology; D009891:Opisthorchis / Q000276:immunology; D011223:Praziquantel / Q000627:therapeutic use; D013785:Thailand; D013997:Time Factors
D000909:Antibodies, Helminth; D000980:Antiplatyhelmintic Agents; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D011223:Praziquantel