Immunological responses to phospholipase-A of Salmonella typhi.
A 39 kD phospholipase-A was purified from Salmonella typhi. The enzyme was efficiently concentrated by precipitation with ammonium sulphate and purified by sequential use of column chromatography on DEAE-Sephacel and Sephadex G-100. Humoral and cellular immune responses were determined against both crude and purified phospholipase-A. Crude fraction was found to be more immunogenic than purified fraction.
Punj
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Department of Microbiology, College of Basic Sciences, Himachal Pradesh Agricultural University, Palampur, India.
Matta
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H
Kalra
M S
MS
D000818:Animals; D005260:Female; D007114:Immunization; D008297:Male; D051379:Mice; D010741:Phospholipases A / Q000276:immunology* / Q000302:isolation & purification; D012485:Salmonella typhi / Q000201:enzymology* / Q000276:immunology
D010741:Phospholipases A