DNA separation with field inversion capillary electrophoresis.
We have built an automated pulsed field capillary electrophoresis system on the basis of a commercially available device. Using entangled polymer solutions as separating matrix, we demonstrate a considerable improvement of separation of double-stranded DNA in the range of 1-50 kbp under pulsed field conditions. The influence of the main parameters, i.e. pulse frequency and electric field, is studied and the results are compared to existing electrophoresis theories.
Heller
C
C
Laboratoire de Physique des Surfaces et Interfaces (URA CNRS 1379), Institut Curie, Paris, France.
Pakleza
C
C
Viovy
J L
JL
D001322:Autoanalysis; D017122:Bacteriophage T4 / Q000235:genetics; D010582:Bacteriophage lambda / Q000235:genetics; D010584:Bacteriophage phi X 174 / Q000235:genetics; D004279:DNA, Viral / Q000302:isolation & purification*; D004560:Electricity; D004586:Electrophoresis; D019075:Electrophoresis, Capillary / Q000379:methods* / Q000706:statistics & numerical data; D008956:Models, Chemical; D011108:Polymers; D012996:Solutions; D013997:Time Factors
D004279:DNA, Viral; D011108:Polymers; D012996:Solutions