The response of human gingival fibroblasts to interleukin-1 in their metabolic conversion of two androgenic substrates.
The effects of interleukin-1 (IL-1) on the metabolism of two androgenic substrates, testosterone and 4-androstenedione, that can be converted to the potent anabolic metabolite 5 alpha-dihydrotestosterone (DHT) were studied. Duplicate incubations of confluent gingival fibroblasts were made in Eagle's minimum essential medium + 10% fetal calf serum, with [14C]-testosterone/[14C]-androstenedione as initial substrates and optimal stimulatory concentrations of IL-1. [14C]-testosterone was converted mainly to DHT and 4-androstenedione. There was a 1.8-fold increase in DHT synthesis (n = 7; p < 0.02) and a 1.9-fold increase in 4-androstenedione in response to IL-1 (n = 7; p < 0.02) (Wilcoxon signed-rank test for non-parametric, paired observations). [14C]-4-androstenedione was mainly converted to DHT and testosterone. There was a 1.7-fold increase in DHT synthesis in response to IL-1 (n = 8; p < 0.01), and a 1.5-fold increase in testosterone formation from [14C]-4-androstenedione in the presence of IL-1, in six of the cell lines (n = 6; p < 0.05). Both testosterone and 4-androstenedione were effective substrates in forming the potent metabolite DHT in significant amounts in response to IL-1. This is significant in view of the well-documented effects of androgens on connective tissue and bone matrix, and may have implications for the healing potential of lesions in inflammatory periodontal disease.
Department of Periodontology, King's College School of Medicine and Dentistry, London, England.
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Kasasa
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Soory
D000328:Adult; D000728:Androgens / Q000378:metabolism*; D000735:Androstenedione / Q000378:metabolism; D002478:Cells, Cultured; D003238:Connective Tissue / Q000502:physiology; D013196:Dihydrotestosterone / Q000378:metabolism; D005260:Female; D005347:Fibroblasts / Q000276:immunology / Q000378:metabolism; D005881:Gingiva / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D005891:Gingivitis / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D007375:Interleukin-1 / Q000502:physiology*; D008297:Male; D012038:Regeneration; D018709:Statistics, Nonparametric; D013739:Testosterone / Q000378:metabolism
D000728:Androgens; D007375:Interleukin-1; D013196:Dihydrotestosterone; D013739:Testosterone; D000735:Androstenedione