Interference of nucleases in cyanobacterium ferredoxin purification.
Isolation of cyanobacterial ferredoxin is normally carried out using nucleases in order to degrade the nucleic acids that accompany this protein during the purification procedure. However, this practice presents the inconvenience that these proteins remain in trace amounts in the purified ferredoxin preparations, although they are not visible by electrophoretical techniques. Evidence of that fact is shown in this report and an alternative procedure is described for the rapid preparation of ferredoxin from crude extracts of Anabaena PCC 7119. The method involves a treatment of the crude extract with streptomycin sulphate, a high molecular weight polication that precipitates the nucleic acids in the beginning of the purification.
Departamento de Bioquímica y Biología Molecular y Celular, Facultad de Ciencias, Universidad de Zaragoza, Spain.
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Razquin
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Gómez-Moreno
D017033:Anabaena / Q000737:chemistry* / Q000201:enzymology; D011232:Chemical Precipitation; D002848:Chromatography, DEAE-Cellulose; D003851:Deoxyribonucleases / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D005288:Ferredoxins / Q000302:isolation & purification*; D005779:Immunodiffusion; D012260:Ribonucleases / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
D005288:Ferredoxins; D003851:Deoxyribonucleases; D012260:Ribonucleases