Correlation of melanoma cell motility and invasion in vitro.
Cell motility and the ability to grow invasively are crucial properties within the metastatic cascade. The relation of cell motility in vitro and metastatic behaviour of tumour cells in animal experiments indicates that they are directly correlated. We undertook this study to see whether a quantitative correlation could be found in complex in vitro systems. Using the assay of directional migration and a newly developed image analysis system to measure cell motility of K1735-M2 mouse melanoma cells and the embryonic chick heart assay of Mareel to follow invasion, we examined the influence of eight compounds on cell motility seven compounds on invasion. For stationary motility we calculated the change of density, area of change, area of ruffling sites (representing only changes at the leading edge and tail of the cell), number of ruffling sites, area of changing intracellular particles and number of intracellular particles. Velocity of single tumour cells and directional migration were also measured. In the invasion assay the parameters STRCSTR and INVASLOG, expressing different forms of stromal (i.e. embryonic chick heart) disintegration and degradation, were calculated. Directional migration and all parameters of stationary motility except number of ruffling sites, changing intracellular particles and number of changing intracellular particles correlated significantly (p < 0.05) with STRCSTR and INVASLOG. For velocity, area of change and area of ruffling we found the most significant correlation with parameters of invasion indicating that both stationary and translocative motility contribute to invasion. Our systems also showed that the compounds tested exerted differential effects on various aspects of motility.(ABSTRACT TRUNCATED AT 250 WORDS)
Department of Dermatology, University of Graz, Austria.
R
R
Hofmann-Wellenhof
R
R
Fink-Puches
J
J
Smolle
C
C
Helige
H A
HA
Tritthart
H
H
Kerl
D000818:Animals; D002121:Calcium Channel Blockers / Q000494:pharmacology; D002465:Cell Movement; D002478:Cells, Cultured; D002642:Chick Embryo; D003572:Cytochalasins / Q000494:pharmacology; D003868:Dequalinium / Q000494:pharmacology; D005260:Female; D005444:Flunarizine / Q000494:pharmacology; D007091:Image Processing, Computer-Assisted; D008546:Melanoma, Experimental / Q000473:pathology* / Q000503:physiopathology; D051379:Mice; D008809:Mice, Inbred C3H; D009206:Myocardium / Q000166:cytology; D009361:Neoplasm Invasiveness; D018874:Spheroids, Cellular; D014407:Tumor Cells, Cultured; D014700:Verapamil / Q000031:analogs & derivatives / Q000494:pharmacology
D002121:Calcium Channel Blockers; D003572:Cytochalasins; C031218:cytochalasin A; D014700:Verapamil; D003868:Dequalinium; D005444:Flunarizine