Crystallization and preliminary crystallographic analysis of recombinant abrin-a A-chain with ribosome inactivating activity.
Crystals have been obtained for a recombinant abrin-a A-chain produced by E. coli. The crystals were grown using PEG6000 as the precipitating agent. The crystals belong to an orthrhombic space group (P2(1)2(1)2(1)) and diffract to 1.7 A.
Department of BioEngineering, Nagaoka University of Technology, Niigata, Japan.
T
T
Kohno
T
T
Senda
H
H
Narumi
S
S
Kimura
Y
Y
Mitsui
D000036:Abrin / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D003460:Crystallization; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry* / Q000302:isolation & purification; D012270:Ribosomes / Q000187:drug effects / Q000378:metabolism*; D013329:Structure-Activity Relationship
D011994:Recombinant Proteins; D000036:Abrin