Cytotoxicity evaluation of six root canal sealers.
Cell cultures of human gingival fibroblasts obtained from healthy patients were used to evaluate the toxicity of six different endodontic cements: AH-26, Pulp Canal Sealer, Rocanal-R2, Rocanal-R3, Bioseal, and Endomethasone. The toxicity was determined by measuring spectrophotometrically at 405 nm the colorimetric reaction of N-acetyl-beta-hexosaminidase, an endogenous enzyme, with the chromogenic substrate [p-nitrophenol-N-acetyl-beta-D-glucosamide (NAG)]. Severe cytotoxicity was observed in the 1- and 2-wk test solutions of AH-26. Pulp Canal Sealer and Endomethasone showed low cytotoxicity in the 1- and 2-wk test solutions at 24, 48, and 72 h. Moderate cytotoxicity was observed in the 1- and 2-wk test solutions of Bioseal, except at 48 and 72 h of 1-wk test solutions. Rocanal-R2 showed severe cytotoxicity in the 1-wk test solutions at 48 and 72 h, and in the 2-wk test solutions at 24, 48, and 72 h. Moderate cytotoxicity was seen in the 1- and 2-wk test solutions of Rocanal-R3 only at 24 h.
Department of Endodontics and Restorative Dentistry, University of Verona, Italy.
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Gerosa
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Menegazzi
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Borin
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Cavalleri
D000287:Administration, Topical; D000893:Anti-Inflammatory Agents / Q000633:toxicity; D001729:Bismuth / Q000633:toxicity; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003124:Colorimetry; D003907:Dexamethasone / Q000633:toxicity; D004338:Drug Combinations; D004853:Epoxy Resins; D005347:Fibroblasts / Q000187:drug effects / Q000201:enzymology; D005557:Formaldehyde / Q000633:toxicity; D005881:Gingiva / Q000166:cytology / Q000187:drug effects / Q000201:enzymology; D006801:Humans; D006854:Hydrocortisone; D008709:Methenamine / Q000633:toxicity; D010636:Phenols / Q000633:toxicity; D012387:Root Canal Filling Materials / Q000633:toxicity*; D012834:Silver / Q000633:toxicity; D013943:Thymol / Q000031:analogs & derivatives / Q000633:toxicity; D014025:Titanium / Q000633:toxicity; D015035:Zinc Oxide-Eugenol Cement / Q000633:toxicity; D001619:beta-N-Acetylhexosaminidases / Q000378:metabolism
D000893:Anti-Inflammatory Agents; C097428:Bioseal; D004338:Drug Combinations; D004853:Epoxy Resins; D010636:Phenols; C097429:Rocanal; D012387:Root Canal Filling Materials; D015035:Zinc Oxide-Eugenol Cement; C011244:corticosteroid methanetriol mixture; D005557:Formaldehyde; D013943:Thymol; D012834:Silver; C028044:epoxy resin AH-26; D003907:Dexamethasone; C076543:Kerr Pulp Canal Sealer; D014025:Titanium; D001619:beta-N-Acetylhexosaminidases; D008709:Methenamine; D001729:Bismuth; D006854:Hydrocortisone