[The isolation and characteristics of a protein from human and bovine kidneys close in molecular and biochemical properties to non-histone chromosomal antigen PP23].
By ion-exchange gradient chromatography, autophosphorylated protein fractions were separated from nonhistone chromosomal protein (NHCP) samples isolated from bovine and human kidney and liver. Fractions, eluted from the phosphocellulose column with 0.4-0.5 M NaCl, could occur only in the case of kidney rather than liver NHCP-fractionation. The molecular weight of the major phosphorylated protein component, eluted in this zone, is about 20-26 kDa, which is close to the molecular weight of protein pp23, previously observed during similar fractionation of NHCP from rat kidney rather than from liver.
A B
AB
Grandilevskaia
A A
AA
Klimova
V P
VP
Kushner
D000818:Animals; D015478:Antigens, Heterophile / Q000032:analysis / Q000302:isolation & purification*; D000951:Antigens, Neoplasm / Q000032:analysis / Q000302:isolation & purification*; D002417:Cattle; D002852:Chromatography, Ion Exchange / Q000379:methods; D002868:Chromosomal Proteins, Non-Histone / Q000276:immunology*; D005260:Female; D006801:Humans; D007668:Kidney / Q000276:immunology*; D008099:Liver / Q000276:immunology; D008297:Male; D008970:Molecular Weight; D051381:Rats
D015478:Antigens, Heterophile; D000951:Antigens, Neoplasm; D002868:Chromosomal Proteins, Non-Histone
Vydelenie i kharakteristika belka iz pochek cheloveka i byka, blizkogo po molekuliarno-biokhimicheskim svoĭstvam NGB-antigenu PP23.