Sequencing peptides without scanning the reflectron: post-source decay with a curved-field reflectron time-of-flight mass spectrometer.
A time-of-flight mass spectrometer has been developed using a reflectron in which the voltages placed on the lens elements correspond to a function describing the arc of a circle. This curved-field reflectron enables acquisition of focused product-ion mass spectra at a single reflectron voltage. In this paper, we present results from this instrument for the amino acid sequencing of several peptides and describe the method of mass calibration.
Cordero
M M
MM
Middle Atlantic Mass Spectrometry Laboratory, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Cornish
T J
TJ
Cotter
R J
RJ
Lys
I A
IA
D000595:Amino Acid Sequence; D000804:Angiotensin II / Q000032:analysis; D002138:Calibration; D002364:Caseins / Q000032:analysis; D008969:Molecular Sequence Data; D010455:Peptides / Q000032:analysis*; D011199:Potentiometry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000295:instrumentation; D013373:Substance P / Q000032:analysis
D002364:Caseins; D010455:Peptides; D000804:Angiotensin II; D013373:Substance P