The white gene of Ceratitis capitata: a phenotypic marker for germline transformation.
Reliable germline transformation is required for molecular studies and ultimately for genetic control of economically important insects, such as the Mediterranean fruit fly (medfly) Ceratitis capitata. A prerequisite for the establishment and maintenance of transformant lines is selectable or phenotypically dominant markers. To this end, a complementary DNA clone derived from the medfly white gene was isolated, which showed substantial similarity to white genes in Drosophila melanogaster and other Diptera. It is correlated with a spontaneous mutation causing white eyes in the medfly and can be used to restore partial eye color in transgenic Drosophila carrying a null mutation in the endogenous white gene.
Zwiebel
L J
LJ
European Molecular Biology Laboratory (EMBL), Heidelberg, Germany.
Saccone
G
G
Zacharopoulou
A
A
Besansky
N J
NJ
Favia
G
G
Collins
F H
FH
Louis
C
C
Kafatos
F C
FC
D018528:ATP-Binding Cassette Transporters; D000595:Amino Acid Sequence; D000818:Animals; D030801:Animals, Genetically Modified; D001483:Base Sequence; D003001:Cloning, Molecular; D004175:Diptera / Q000737:chemistry / Q000235:genetics*; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000235:genetics; D005127:Eye Color / Q000235:genetics; D005136:Eye Proteins / Q000737:chemistry / Q000235:genetics*; D017344:Genes, Insect; D005819:Genetic Markers; D007301:Insect Hormones / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D009154:Mutation; D010641:Phenotype; D016415:Sequence Alignment; D014170:Transformation, Genetic
D018528:ATP-Binding Cassette Transporters; D029721:Drosophila Proteins; D005136:Eye Proteins; D005819:Genetic Markers; D007301:Insect Hormones; C059254:w protein, Drosophila