Radioimmunodetection of human leukemia with anti-interleukin-2 receptor antibody in severe combined immunodeficiency mice.
Anti-Tac monoclonal antibody recognizes human interleukin-2 receptor, which is overexpressed in leukemic cells of most adult T-cell leukemia (ATL) patients. To examine the potency of anti-Tac for targeting of ATL, biodistributions of intravenously administered 125I- and 111In-labeled anti-Tac were examined in severe combined immunodeficiency (SCID) mice inoculated with ATL cells. Significant amounts of radiolabeled anti-Tac were found in the spleen and thymus. The trafficking of ATL cells in SCID mice was detected using 111In-oxine-labeled ATL cells. These results were coincident with the histologically confirmed infiltration of ATL cells. The radiolabeled anti-Tac seemed potent for targeting of ATL.
Department of Nuclear Medicine, Faculty of Medicine, Kyoto University, Japan.
M
M
Hosono
A
A
Takaori-Kondo
Z S
ZS
Yao
H
H
Kobayashi
M N
MN
Hosono
H
H
Sakahara
K
K
Imada
M
M
Okuma
T
T
Uchiyama
J
J
Konishi
D000328:Adult; D000818:Animals; D000911:Antibodies, Monoclonal / Q000493:pharmacokinetics; D006801:Humans; D018796:Immunoconjugates / Q000378:metabolism / Q000493:pharmacokinetics; D007205:Indium Radioisotopes; D007457:Iodine Radioisotopes; D015458:Leukemia, T-Cell / Q000000981:diagnostic imaging* / Q000378:metabolism; D051379:Mice; D016513:Mice, SCID; D009942:Organometallic Compounds / Q000378:metabolism; D015125:Oxyquinoline / Q000031:analogs & derivatives / Q000378:metabolism; D016719:Radioimmunodetection; D015375:Receptors, Interleukin-2 / Q000276:immunology; D014018:Tissue Distribution
D000911:Antibodies, Monoclonal; D018796:Immunoconjugates; D007205:Indium Radioisotopes; D007457:Iodine Radioisotopes; D009942:Organometallic Compounds; D015375:Receptors, Interleukin-2; C032950:indium oxine; D015125:Oxyquinoline