Determination of aflatoxins in dust and urine by liquid chromatography/electrospray ionization tandem mass spectrometry.
A liquid chromatography/electrospray ionization tandem mass spectrometry method is described for the determination of aflatoxins B1, B2, G1 and G2. Samples of naturally contaminated airborne dust and spiked urine were cleaned up on immunoaffinity columns and analysed by liquid chromatography using either mass spectrometry detection or post-column derivatization with bromine and fluorescence detection. With tandem mass spectrometry, detection limits (S/N = 3) calculated as amount ejected on column were: aflatoxin B1 4 pg, B2 4 pg, G1 5 pg, and G2 10 pg.
National Institute for Working Life, Analytical Chemistry Division, Umeå, Sweden.
A
A
Kussak
C A
CA
Nilsson
B
B
Andersson
J
J
Langridge
D000348:Aflatoxins / Q000032:analysis* / Q000652:urine; D002853:Chromatography, Liquid; D004391:Dust / Q000032:analysis*; D006801:Humans; D007202:Indicators and Reagents; D013058:Mass Spectrometry; D013050:Spectrometry, Fluorescence
D000348:Aflatoxins; D004391:Dust; D007202:Indicators and Reagents