Functional brain block preparation of the rat auditory cortex.
To maintain neural functions in brain block preparations of the rat auditory cortex in vitro, a pressurized oxygenated medium was injected into the blocks. Distribution of indigo carmine contained in the injection medium indicated that a columnar region of 1-2 mm in diameter was homogeneously perfused from the white matter to the pial surface. Stimulation of cortical layers just above the white matter produced supragranular field potentials of two negative peaks. They represented antidromic and postsynaptic activities, of which only the latter was blocked by 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX, 10 microM). The depth profile and temperature-dependency of field potentials in the blocks were very similar to those recorded in usual slice preparations. The responses in blocks were recorded stably for several hours. The functional brain block preparation may be a useful tool for analyses of neocortical neural networks in vitro.
Fujisaki
T
T
Department of Neurophysiology, Niigata University, Japan.
Kudoh
M
M
Shibuki
K
K
D018750:6-Cyano-7-nitroquinoxaline-2,3-dione / Q000494:pharmacology; D000818:Animals; D001303:Auditory Cortex / Q000187:drug effects / Q000502:physiology*; D004558:Electric Stimulation; D004594:Electrophysiology; D005071:Evoked Potentials / Q000502:physiology*; D066298:In Vitro Techniques; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013696:Temperature; D013997:Time Factors
D018750:6-Cyano-7-nitroquinoxaline-2,3-dione