Persistence of a single electropherotype and serotype (G6P5) of bovine rotavirus in calves on a closed dairy farm from 1990 to 1993.
A virologic survey was conducted on calves with diarrhea associated with bovine rotavirus (BRV) on a closed dairy farm. The BRV was detected from 32 of 219 (14.6%) fecal specimens repeatedly collected from 56 calves born during the years 1992-1993, regardless of whether they had diarrhea. Most of the 32 strains were isolated from fecal specimens obtained from 2- to 6-week-old calves. After electrophoresis of double-stranded viral RNA from the 32 strains, genomic RNA migration patterns were similar to those of the predominant BRV strains isolated at the same farm during the years 1990-1991. All representative strains were identified as G serotype 6 (G6) and P type 5 (P5) by results of the virus-neutralization test and polymerase chain reaction procedure. Thus, BRV had no change in genomic RNA electropherotypes and serologic antigenicities in a closed dairy herd over a period of several years.
Department of Veterinary Microbiology, Obihiro University of Agriculture and Veterinary Medicine, Japan.
H
H
Ishizaki
C
C
Ohta
T
T
Shirahata
H
H
Goto
K
K
Taniguchi
T
T
Urasawa
S
S
Urasawa
D000818:Animals; D000831:Animals, Newborn / Q000276:immunology / Q000821:virology; D000956:Antigens, Viral / Q000276:immunology; D001483:Base Sequence; D002417:Cattle / Q000821:virology*; D017931:DNA Primers; D003612:Dairying; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D005260:Female; D008969:Molecular Sequence Data; D009500:Neutralization Tests / Q000662:veterinary; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012330:RNA, Double-Stranded / Q000302:isolation & purification; D012401:Rotavirus / Q000145:classification / Q000302:isolation & purification*; D012703:Serotyping / Q000662:veterinary
D000956:Antigens, Viral; D017931:DNA Primers; D012330:RNA, Double-Stranded