The occurrence of natural antibodies to minimal component of bacterial cell wall (N-acetylglucosaminyl-N-acetylmuramyl dipeptide) in sera from healthy humans.
ELISA assay showed that sera from each of 729 healthy donors contained antibodies to the minimal component of bacterial cell walls, N-acetylglucosaminyl-N-acetylmuramyl dipeptide (GMDP). Anti-GMDP antibody levels were determined for 686 sera which were classified into 3 groups: high (17.6%), medium (68.7%), and low responder (13.7%). Inhibition analysis performed on representative sera showed that a proportion contained specific anti-GMDP antibodies reacting only with GMDP (i.e., GMDP interaction with anti-GMDP antibodies was inhibited by GMDP only) whereas the remaining sera reacted both with GMDP and with the tetrasaccharide (GlcNAc-MurNAc)2 (i.e., GMDP interaction with anti-GMDP antibodies in the latter sera was inhibited by both GMDP and the tetrasaccharide). Inhibition analysis indicated, moreover, that the anti-GMDP antibodies contained in high-responder sera had higher affinity than those present in low-responder ones: GMDP inhibited the GMDP + anti-GMDP antibody interaction by 88.7% in the former sera vs. 53% in the latter. Sera contained both IgM and IgG antibodies to GDMP, but the mean level of anti-GMDP IgG antibodies in the high-responder sera was 30 times higher than in the low-responder ones.
Pinegin
B V
BV
Institute of Immunology, Russian Ministry of Health, Moscow.
Kulakov
A V
AV
Makarov
E A
EA
Ledger
P W
PW
Khaitov
R M
RM
D000119:Acetylmuramyl-Alanyl-Isoglutamine / Q000031:analogs & derivatives* / Q000276:immunology; D000907:Antibodies, Bacterial / Q000097:blood*; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions; D001426:Bacterial Proteins / Q000276:immunology*; D002473:Cell Wall / Q000276:immunology; D004306:Dose-Response Relationship, Immunologic; D006801:Humans; D007113:Immunity, Innate; D010457:Peptidoglycan / Q000276:immunology / Q000494:pharmacology
D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D010457:Peptidoglycan; D000119:Acetylmuramyl-Alanyl-Isoglutamine; C023868:N-acetyl-beta-glucosaminyl-N-acetylmuramyl-alanylisoglutamine