Mitochondrial ATP synthase alpha-subunit gene amplified in a retinoblastoma cell line maps to chromosome 18.
The human retinoblastoma cell line Y79 has multiple copies of the MYCN gene and the DEAD box gene DDXI. Both genes have been mapped to chromosome band 2p24. A third gene, encoding the alpha-subunit of mitochondrial ATP synthase (ATPSA), is also amplified in Y79. Here we report that there are at least four human mitochondrial ATPSA-related genes located on four different chromosomes. The ATPSA gene that is amplified in Y79 originates from chromosome 18. In Y79, the amplified copies of both the ATPSA and the MYCN genes are located on a homogeneously staining region (HSR) at chromosome band Ip34.
Molecular Oncology Program, Cross Cancer Institute, Edmonton, Alberta, Canada.
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W
Bie
J A
JA
Squire
M
M
Fraser
M C
MC
Paterson
R
R
Godbout
D000818:Animals; D002874:Chromosome Mapping; D002885:Chromosomes, Human, Pair 16; D002887:Chromosomes, Human, Pair 18; D002889:Chromosomes, Human, Pair 2; D002899:Chromosomes, Human, Pair 9; D006224:Cricetinae; D005784:Gene Amplification; D016259:Genes, myc; D006801:Humans; D051379:Mice; D008928:Mitochondria / Q000201:enzymology*; D006180:Proton-Translocating ATPases / Q000235:genetics*; D051381:Rats; D012175:Retinoblastoma / Q000201:enzymology* / Q000235:genetics*; D014407:Tumor Cells, Cultured
D006180:Proton-Translocating ATPases