Molecular cloning, characterization and expression of an elongation factor 1 alpha gene in maize.
A cDNA (zmEF1A) and the corresponding genomic clone (zmgEF1A) of a member of the gene family encoding the alpha subunit of translation elongation factor 1 (EF-1 alpha) have been isolated from maize. The deduced amino acid sequence is 447 residues long interrupted by one intron. Southern blot analysis reveals that the cloned EF-1 alpha gene is one member out of a family consisting of at least six genes. As shown by northern hybridizations in leaves the mRNA level increases at low temperature whereas time-course experiments over 24 h at 5 degrees C show that in roots the overall mRNA level of EF-1 alpha is transiently decreased. These results indicate that the expression of EF-1 alpha is differently regulated in leaves and roots under cold stress.
Laboratory of Plant Genetic Engineering, Akita Prefectural College of Agriculture, Japan.
T
T
Berberich
K
K
Sugawara
M
M
Harada
T
T
Kusano
D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D018076:DNA, Complementary; D018744:DNA, Plant / Q000302:isolation & purification / Q000378:metabolism; D015870:Gene Expression; D017343:Genes, Plant; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D005810:Multigene Family; D020648:Peptide Elongation Factor 1; D010445:Peptide Elongation Factors / Q000096:biosynthesis* / Q000235:genetics; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics; D018517:Plant Roots; D012333:RNA, Messenger / Q000302:isolation & purification / Q000378:metabolism; D003313:Zea mays / Q000235:genetics* / Q000378:metabolism*
D018076:DNA, Complementary; D018744:DNA, Plant; D046911:Macromolecular Substances; D020648:Peptide Elongation Factor 1; D010445:Peptide Elongation Factors; D010940:Plant Proteins; D012333:RNA, Messenger