Transcriptional expression of mannose receptor gene during differentiation of human macrophages.
Mannose receptor is a differentiation marker of macrophages. Circulating monocytes isolated from plasma are devoid of this receptor; upon culture this receptor is rapidly expressed. Its expression is modulated by a variety of inflammatory and anti-inflammatory agents. In the present study, we investigated its transcription level during the differentiation process. Mannose receptor mRNA was monitored by quantitative RT-PCR on freshly harvested monocytes and on monocytes cultivated up to four days. No transcription was detected in freshly harvested cells, the transcription increased during the first 24 h upon adhesion and then decreased.
Centre de Biophysique Moléculaire, CNRS, Orléans, France.
F
F
Rouleux-Bonnin
M
M
Monsigny
A
A
Legrand
D000943:Antigens, Differentiation / Q000235:genetics; D001483:Base Sequence; D002448:Cell Adhesion; D002454:Cell Differentiation; D017931:DNA Primers / Q000235:genetics; D037502:Galectin 3; D015870:Gene Expression; D006801:Humans; D066298:In Vitro Techniques; D037181:Lectins, C-Type; D008264:Macrophages / Q000166:cytology / Q000378:metabolism*; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins; D008969:Molecular Sequence Data; D009000:Monocytes / Q000166:cytology / Q000378:metabolism; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000235:genetics*
D000943:Antigens, Differentiation; D017931:DNA Primers; D037502:Galectin 3; D037181:Lectins, C-Type; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins; D012333:RNA, Messenger; D011956:Receptors, Cell Surface