Stable integration of vectors at high copy number for high-level expression in animal cells.
Barsoum
J
J
Biogen Inc., Cambridge, MA, USA.
D000818:Animals; D016466:CHO Cells; D002874:Chromosome Mapping; D006224:Cricetinae; D004247:DNA / Q000008:administration & dosage / Q000235:genetics; D018274:Electroporation / Q000379:methods*; D005784:Gene Amplification; D015870:Gene Expression; D005822:Genetic Vectors; D008727:Methotrexate / Q000494:pharmacology; D010957:Plasmids / Q000008:administration & dosage / Q000235:genetics; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics; D013762:Tetrahydrofolate Dehydrogenase / Q000235:genetics; D014162:Transfection
D011506:Proteins; D004247:DNA; D013762:Tetrahydrofolate Dehydrogenase; D008727:Methotrexate