Editing of the chloroplast ndhB encoded transcript shows divergence between closely related members of the grass family (Poaceae).
The ndhB-encoded transcript from barley chloroplasts deviates from the genomic ndhB sequence by nine C-to-U transitions, which is the maximum number of editing events for a chloroplast mRNA reported so far. Comparison with ndhB transcripts from other chloroplast species shows that six of the nine editing sites observed in barley are structurally and functionally conserved in maize, rice and tobacco. The remaining three sites, however, show divergent patterns of conservation even within the three members of the grass family. The conservation of two of these sites in tobacco but not in the closely related graminean species suggests that divergence of the ndhB editing sites is caused by the loss of preexisting editing sites rather than by gain of new sites.
Institut für Biologie III, Universität Freiburg, Germany.
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Freyer
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López
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Maier
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Martín
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Sabater
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Kössel
D000595:Amino Acid Sequence; D001483:Base Sequence; D005075:Biological Evolution; D002736:Chloroplasts / Q000201:enzymology / Q000235:genetics*; D018076:DNA, Complementary; D018744:DNA, Plant; D002523:Edible Grain / Q000201:enzymology / Q000235:genetics*; D001467:Hordeum / Q000201:enzymology / Q000235:genetics; D008969:Molecular Sequence Data; D012275:Oryza / Q000201:enzymology / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics*; D006109:Poaceae / Q000201:enzymology / Q000235:genetics*; D016133:Polymerase Chain Reaction; D017393:RNA Editing; D012333:RNA, Messenger / Q000235:genetics; D018749:RNA, Plant / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D014158:Transcription, Genetic
D018076:DNA, Complementary; D018744:DNA, Plant; D010940:Plant Proteins; D012333:RNA, Messenger; D018749:RNA, Plant