Cloning and sequencing of the membrane-bound hydrogenase-encoding genes (hupS and hupL) from Pseudomonas hydrogenovora.
The membrane-bound hydrogenase (Hdg)-encoding structural genes were isolated from the hydrogen-utilizing bacterium Pseudomonas hydrogenovora (Ph). Nucleotide sequence analysis revealed two genes, hupS and hupL. The hupS gene encoded a 363-amino-acid (aa) polypeptide (40.4 kDa). The deduced aa sequence contained a putative 43-aa leader peptide sequence. The hupL gene started at 55 bp downstream from the stop codon of hupS and encoded a 622-aa polypeptide (69.3 kDa). The disruption of Ph hupS resulted in the loss of Hdg activity.
Ohtsuki
T
T
Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, Nagano, Japan.
Okazaki
T
T
Endo
Y
Y
Kita
Y
Y
Shimosaka
M
M
Okazaki
M
M
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D002462:Cell Membrane / Q000201:enzymology; D003001:Cloning, Molecular; D005798:Genes, Bacterial; D006864:Hydrogenase / Q000235:genetics*; D008565:Membrane Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D010088:Oxidoreductases; D011401:Promoter Regions, Genetic; D011549:Pseudomonas / Q000235:genetics*; D015183:Restriction Mapping; D012808:Sigma Factor / Q000235:genetics
D001426:Bacterial Proteins; D008565:Membrane Proteins; D012808:Sigma Factor; D010088:Oxidoreductases; C091789:HupL protein, Anabaena; D006864:Hydrogenase