Detection of MspI RFLP in human THY1 gene by the polymerase chain reaction.
THY1 gene encodes a cell surface glycoprotein predominantly expressed in brain and peripheral nerves. Human THY1 gene region on chromosome 11q23 has been implicated in susceptibility to type 1 diabetes (Wong et al., 1991). Two primers derived from the sequences flanking the polymorphic MspI site in intron 2 of the human THY1 gene (Gatti et al., 1988) were selected for RCP to amplify a 566 bp fragment that spans the MspI polymorphism. Polymorphism was detected by MspI digestion of the PCR product.
Department of Geriatric Medicine, Osaka University Medical School, Suita, Japan.
J
J
Fu
H
H
Ikegami
E
E
Yamato
Y
Y
Kawaguchi
K
K
Takekawa
T
T
Fujisawa
Y
Y
Nakagawa
Y
Y
Hamada
H
H
Ueda
G Q
GQ
Shen
D001483:Base Sequence; D019003:Deoxyribonuclease HpaII; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D018800:Thy-1 Antigens / Q000235:genetics*
D018800:Thy-1 Antigens; D019003:Deoxyribonuclease HpaII