A feline herpesvirus-1 recombinant with a deletion in the genes for glycoproteins gI and gE is effective as a vaccine for feline rhinotracheitis.
Using a site-directed mutagenesis technique we constructed a new feline herpesvirus-1 recombinant strain containing a deletion in two genes encoding glycoproteins gI and gE. These proteins may have a role in virulence, the establishment of latency, and viral recurrence as shown in other herpesviruses of the varicella and simplex types. This recombinant was characterized and used to immunize juvenile cats against virulent virus challenge. Significant protection resulted from vaccination of cats by the subcutaneous route.
Sussman
M D
MD
Department of Microbiology, Michigan State University, East Lansing 44824, USA.
Maes
R K
RK
Kruger
J M
JM
Spatz
S J
SJ
Venta
P J
PJ
D018138:Alphaherpesvirinae / Q000235:genetics* / Q000276:immunology; D000818:Animals; D001483:Base Sequence; D002371:Cat Diseases / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D002415:Cats; D002460:Cell Line; D017931:DNA Primers; D004279:DNA, Viral; D017353:Gene Deletion; D006023:Glycoproteins / Q000235:genetics*; D006566:Herpesviridae Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D008969:Molecular Sequence Data; D014136:Tracheitis / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary* / Q000821:virology; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics*; D014765:Viral Vaccines / Q000008:administration & dosage / Q000235:genetics*; D001616:beta-Galactosidase / Q000235:genetics
D017931:DNA Primers; D004279:DNA, Viral; D006023:Glycoproteins; D014614:Vaccines, Synthetic; D014764:Viral Proteins; D014765:Viral Vaccines; D001616:beta-Galactosidase