Monitoring of spatial expression of firefly luciferase in transformed zebrafish.
The firefly luciferase gene attached to the cytomegalovirus promoter was transferred into zebrafish (Brachydanio rerio) by microinjection of fertilized eggs. Light emission could be monitored in vivo in eggs and throughout the early development of the fish by low-light video-image analysis. Gene expression was transient in most of the cases lasting for about 2 weeks. This gene cassette proved to be a very convenient and non-destructive transformation marker and the firefly luciferase gene appears to be a powerful tool for real-time imaging of tissue-specific gene expression in transgenic fish.
Department of Plant Science, University of Alberta, Edmonton, Canada.
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Mayerhofer
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Araki
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Szalay
D000818:Animals; D030801:Animals, Genetically Modified; D015139:Blotting, Southern; D001517:Coleoptera / Q000201:enzymology*; D003587:Cytomegalovirus; D004247:DNA / Q000032:analysis; D005260:Female; D015870:Gene Expression; D005819:Genetic Markers; D008156:Luciferases / Q000096:biosynthesis* / Q000235:genetics; D009865:Oocytes / Q000201:enzymology; D011401:Promoter Regions, Genetic; D015027:Zebrafish; D015053:Zygote / Q000166:cytology* / Q000201:enzymology
D005819:Genetic Markers; D004247:DNA; D008156:Luciferases