The use of capillary electrophoresis for the determination of hemoglobin variants.
The application of capillary electrophoresis and related techniques for the detection of hemoglobin variants is described. Capillary zone electrophoresis (CZE) was applied for the analysis of intact tetrameric hemoglobin. CZE under denaturing conditions was used for the separation of globin chains. Both CZE and micellar electrokinetic capillary chromatography were applied for a fast and sensitive separation of tryptic digests and for the analysis of amino acid derivatives.
Institute of Biological Chemistry, University of Cagliari, Italy.
M
M
Castagnola
I
I
Messana
L
L
Cassiano
R
R
Rabino
D V
DV
Rossetti
B
B
Giardina
D000596:Amino Acids / Q000032:analysis; D002845:Chromatography / Q000379:methods; D002851:Chromatography, High Pressure Liquid; D004563:Electrochemistry; D019075:Electrophoresis, Capillary / Q000379:methods*; D005914:Globins / Q000302:isolation & purification; D006441:Hemoglobin A / Q000032:analysis / Q000378:metabolism; D006444:Hemoglobin C / Q000032:analysis / Q000378:metabolism; D006451:Hemoglobin, Sickle / Q000032:analysis / Q000378:metabolism; D006455:Hemoglobins, Abnormal / Q000032:analysis* / Q000378:metabolism; D006801:Humans; D046911:Macromolecular Substances; D008823:Micelles; D010446:Peptide Fragments / Q000032:analysis / Q000378:metabolism; D010449:Peptide Mapping; D014357:Trypsin / Q000378:metabolism
D000596:Amino Acids; D006451:Hemoglobin, Sickle; D006455:Hemoglobins, Abnormal; D046911:Macromolecular Substances; D008823:Micelles; D010446:Peptide Fragments; D005914:Globins; D006444:Hemoglobin C; D006441:Hemoglobin A; D014357:Trypsin