Characterization of the Erwinia chrysanthemi osmoprotectant transporter gene ousA.
Growth of Erwinia chrysanthemi in media of elevated osmolarity can be achieved by the uptake and accumulation of various osmoprotectants. This study deals with the cloning and sequencing of the ousA gene-encoded osmoprotectant uptake system A from E. chrysanthemi 3937. OusA belongs to the superfamily of solute ion cotransporters. This osmotically inducible system allows the uptake of glycine betaine, proline, ectoine, and pipecolic acid and presents strong similarities in nucleotide sequence and protein function with the proline/betaine porter of Escherichia coli encoded by proP. The control of ousA expression is clearly different from that of proP. It is induced by osmotic strength and repressed by osmoprotectants. Its expression in E. coli is controlled by H-NS and is rpoS dependent in the exponential phase but unaffected by the stationary phase.
Gouesbet
G
G
Centre National de la Recherche Scientifique (CNRS) URA 256, Département Membranes et Osmorégulation, Université de Rennes I, France.
Trautwetter
A
A
Bonnassie
S
S
Wu
L F
LF
Blanco
C
C
D000595:Amino Acid Sequence; D000599:Amino Acids, Diamino / Q000378:metabolism; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D001622:Betaine / Q000378:metabolism / Q000494:pharmacology; D001692:Biological Transport; D002352:Carrier Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D003001:Cloning, Molecular; D003470:Culture Media; D004268:DNA-Binding Proteins / Q000235:genetics / Q000502:physiology; D016974:Dickeya chrysanthemi / Q000235:genetics* / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development; D029968:Escherichia coli Proteins; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects / Q000502:physiology*; D005798:Genes, Bacterial / Q000235:genetics; D005816:Genetic Complementation Test; D005966:Glucuronidase / Q000096:biosynthesis / Q000235:genetics; D026901:Membrane Transport Proteins; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009687:Nuclear Proteins / Q000235:genetics / Q000502:physiology; D009994:Osmolar Concentration; D010875:Pipecolic Acids / Q000378:metabolism; D011392:Proline / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D012808:Sigma Factor / Q000235:genetics; D027981:Symporters
D000599:Amino Acids, Diamino; D001426:Bacterial Proteins; D002352:Carrier Proteins; D003470:Culture Media; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; C031908:H-NS protein, bacteria; D026901:Membrane Transport Proteins; D009687:Nuclear Proteins; C097645:OusA protein, Erwinia chrysanthemi; D010875:Pipecolic Acids; C079244:ProP protein, E coli; D011993:Recombinant Fusion Proteins; D012808:Sigma Factor; D027981:Symporters; C069567:sigma factor KatF protein, Bacteria; D001622:Betaine; C045628:ectoine; D011392:Proline; D005966:Glucuronidase; C031345:pipecolic acid