Low temperature persistence of type I antifreeze protein is mediated by cold-specific mRNA stability.
In winter flounder, the levels of type I antifreeze protein (AFP) and its mRNA vary seasonally by as much as 1000-fold. Elevated levels in the fall are prompted by the loss of long day-lengths, while higher spring temperatures correlate with AFP clearance. We have investigated the role of temperature on AFP accumulation using transgenic Drosophila melanogaster by expressing multiple AFP genes under control of the heat-inducible hsp70 promoter. AFP and AFP mRNA persisted far longer in flies reared at 10 degrees C compared to 22 degrees C. This difference appears to be mediated by cold-specific mRNA stability since no such temperature effect was observed with either an endogenous heat-inducible mRNA or a constitutively expressed mRNA.
Duncker
B P
BP
Department of Biology, Queen's University, Kingston, Ontario, Canada.
Koops
M D
MD
Walker
V K
VK
Davies
P L
PL
D000818:Animals; D030801:Animals, Genetically Modified; D021301:Antifreeze Proteins; D003080:Cold Temperature; D004330:Drosophila / Q000378:metabolism; D005432:Flounder / Q000378:metabolism*; D005786:Gene Expression Regulation; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis*; D012623:Seawater
D021301:Antifreeze Proteins; D006023:Glycoproteins; D012333:RNA, Messenger