Lysosomal glycosidases in different populations of human thymocytes.
Lysosomal glycosidase activities were studied in human thymocyte fractions obtained by two methods: (A) fractionation in Percoll density gradient and (B) separation from the cells forming rosettes with sheep erythrocytes (E-RFC). (A) affords fraction L, enriched with immature and endogenously activated thymocytes, and fraction H containing mature thymocytes. By use of (B), fraction E-RFC--enriched with non-activated immature thymocytes--was obtained. Comparative study of E-RFC and H revealed diverse alterations in activities of glycosidases during thymocytes maturation, specifically decreases in alpha-L-fucosidase and alpha-D-mannosidase and an increase in beta-D-galactosidase. Comparing E-RFC and L demonstrates increases in activities of studied glycosidases following endogenous activation of thymocytes.
Ushakova
N A
NA
Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, Moscow, Russia.
Sirina
E G
EG
Preobrazhenskaya
M E
ME
Yarilin
A A
AA
D000276:Adjuvants, Immunologic / Q000494:pharmacology; D000818:Animals; D015703:Antigens, CD / Q000032:analysis; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D002648:Child; D006026:Glycoside Hydrolases / Q000378:metabolism*; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects; D008247:Lysosomes / Q000201:enzymology*; D011994:Recombinant Proteins / Q000494:pharmacology; D012397:Rosette Formation; D012756:Sheep; D016176:T-Lymphocyte Subsets / Q000166:cytology / Q000201:enzymology* / Q000276:immunology; D013601:T-Lymphocytes / Q000166:cytology / Q000201:enzymology* / Q000276:immunology; D013950:Thymus Gland / Q000201:enzymology*; D013951:Thymus Hormones / Q000494:pharmacology
D000276:Adjuvants, Immunologic; D015703:Antigens, CD; D007376:Interleukin-2; D011994:Recombinant Proteins; D013951:Thymus Hormones; C032805:thymalin; D006026:Glycoside Hydrolases