Transforming growth factor beta 1 inhibits interleukin-10 mRNA expression and production in pokeweed mitogen-stimulated peripheral blood mononuclear cells and T cells.
The multifunctional cytokine transforming growth factor-beta 1 (TGF-beta 1) is known to inhibit the proliferation of lymphocytes. Whether this effect is a result of a direct action of TGF-beta 1 or an involvement of other "immunoinhibitory" cytokines is not yet clear. Here we have analyzed the effects of TGF-beta 1 on IL-10 and IL-1RA production in pokeweed mitogen-stimulated peripheral blood mononuclear cells (PBMC) and purified T lymphocytes. We show in these systems that TGF-beta 1 at a concentration of 10 ng/ml significantly suppresses both IL-10 mRNA expression and IL-10 production. IL-2 and IL-6 were capable of abolishing the effect of TGF-beta 1 on DNA synthesis and production of IL-10 by T lymphocytes in an additive manner. However, TGF-beta 1 did not influence IL-1RA production in PWM-stimulated PBMC. The present data show that the inhibitory effect of TGF-beta 1 on mitogen-activated immune cells is not the consequence of induction of the inhibitory cytokines IL-10 or IL-1RA but rather an inhibitory action on the production of IL-2 and/or IL-6.
Department of Internal Medicine, Otto von Guericke University Magdeburg, Germany.
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Bühling
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Ansorge
D004247:DNA / Q000096:biosynthesis; D006801:Humans; D053590:Interleukin 1 Receptor Antagonist Protein; D007375:Interleukin-1 / Q000235:genetics; D016753:Interleukin-10 / Q000235:genetics*; D007376:Interleukin-2 / Q000494:pharmacology; D015850:Interleukin-6 / Q000494:pharmacology; D007963:Leukocytes, Mononuclear / Q000187:drug effects*; D011043:Pokeweed Mitogens / Q000494:pharmacology*; D012333:RNA, Messenger / Q000096:biosynthesis*; D011994:Recombinant Proteins / Q000235:genetics; D012795:Sialoglycoproteins / Q000235:genetics; D013601:T-Lymphocytes / Q000187:drug effects*; D016212:Transforming Growth Factor beta / Q000037:antagonists & inhibitors / Q000494:pharmacology*
C507763:IL1RN protein, human; D053590:Interleukin 1 Receptor Antagonist Protein; D007375:Interleukin-1; D007376:Interleukin-2; D015850:Interleukin-6; D011043:Pokeweed Mitogens; D012333:RNA, Messenger; D011994:Recombinant Proteins; D012795:Sialoglycoproteins; D016212:Transforming Growth Factor beta; D016753:Interleukin-10; D004247:DNA