Identification of Schizosaccharomyces pombe gene psk1+, encoding a novel putative serine/threonine protein kinase, whose mutation conferred resistance to phenylarsine oxide.
We have identified a novel putative protein kinase-encoding gene from Schizosaccharomyces pombe (Sp), designated psk1+, by using a highly conserved amino acid (aa) sequence motif to design amplification of DNA fragments using PCR. The putative translation product of psk1+ contains 436 aa, with a molecular mass of 49,317 Da. A single psk1+ was identified by genomic Southern blot analysis, and the genomic mapping indicated that psk1+ was localized in Sp chromosome III. Growth of wild-type Sp cells was inhibited by 0.5 microM phenylarsine oxide, a protein tyrosine phosphatase inhibitor, but psk1- cells were relatively resistant to this drug.
Mukai
H
H
Department of Biology, Faculty of Science, Kobe University, Japan.
Miyahara
M
M
Takanaga
H
H
Kitagawa
M
M
Shibata
H
H
Shimakawa
M
M
Ono
Y
Y
D000595:Amino Acid Sequence; D001152:Arsenicals / Q000494:pharmacology; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000235:genetics; D004352:Drug Resistance, Microbial; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005656:Fungal Proteins / Q000235:genetics; D015870:Gene Expression; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*; D017027:Protein Tyrosine Phosphatases / Q000037:antagonists & inhibitors; D012331:RNA, Fungal / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D012568:Schizosaccharomyces / Q000235:genetics*; D029702:Schizosaccharomyces pombe Proteins
D001152:Arsenicals; D004271:DNA, Fungal; D004791:Enzyme Inhibitors; D005656:Fungal Proteins; D012331:RNA, Fungal; D012333:RNA, Messenger; D029702:Schizosaccharomyces pombe Proteins; C029341:oxophenylarsine; C097249:Psk1 protein, S pombe; D017346:Protein Serine-Threonine Kinases; D017027:Protein Tyrosine Phosphatases