Structural and functional homology between periplasmic bacterial molecular chaperones and small heat shock proteins.
The periplasmic Yersinia pestis molecular chaperone Caf1M belongs to a superfamily of bacterial proteins for one of which (PapD protein of Escherichia coli) the immunoglobulin-like fold was solved by X-ray analysis. The N-terminal domain of Caf1M was found to share a 20% amino acid sequence identity with an inclusion body-associated protein IbpB of Escherichia coli. One of the regions that was compared, was 32 amino acids long, and displayed more than 40% identity, probability of random coincidence was 1.2 x 10(-4). IbpB is involved in a superfamily of small heat shock proteins which fulfil the function of molecular chaperone. On the basis of the revealed homology, an immunoglobulin-like one-domain model of IbpB three-dimensional structure was designed which could be a prototype conformation of sHsp's. The structure suggested is in good agreement with the known experimental data obtained for different members of sHsp's superfamily.
Zav'yalov
V P
VP
Institute of Immunology, Moscow Region, Russia.
Zav'yalova
G A
GA
Denesyuk
A I
AI
Gaestel
M
M
Korpela
T
T
D000595:Amino Acid Sequence; D000828:Animal Population Groups / Q000378:metabolism; D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry* / Q000502:physiology; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000737:chemistry*; D029968:Escherichia coli Proteins; D006360:Heat-Shock Proteins / Q000737:chemistry* / Q000502:physiology; D006801:Humans; D018832:Molecular Chaperones / Q000737:chemistry* / Q000502:physiology; D008969:Molecular Sequence Data; D008970:Molecular Weight; D033903:Periplasmic Proteins; D010944:Plants / Q000378:metabolism; D011487:Protein Conformation; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D013329:Structure-Activity Relationship; D015010:Yersinia pestis / Q000737:chemistry*
D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D006360:Heat-Shock Proteins; C097578:IbpA protein, E coli; C097579:IbpB protein, E coli; D018832:Molecular Chaperones; C084330:PapD protein, E coli; D033903:Periplasmic Proteins; C070195:CAf1M protein, Yersinia pestis