[Expression of the gene of cholecystokinin. Demonstration from duodenal biopsies in man].
Cholecystokinin (CCK) is a peptide released after feeding. Until now, CCK gene expression has been studied only on sacrified animals on mucosa scrapped off the duodenum. OBJECTIVE--The aim of this study was to assess CCK-mRNA detection on duodenal biopsy specimens in subjects undergoing gastrointestinal endoscopy. METHODS--Six biopsy specimens were taken from 6 healthy subjects, a) after a 12-h overnight fast and b) 6 or 12 h after a fat meal, and inducing a CCK release. CCK-mRNA was analyzed by Northern blot. RESULTS--Plasma CCK levels increased from basal levels of 0.5 +/- 0.1 pmole/L to 8.2 +/- 1.5 pmole/L 10 min after the meal. The fasting CCK-mRNA levels were 44.0 +/- 0.6% and the post-prandial levels increased to 74.0 +/- 6.0% at 6 h and decreased to 47.5 +/- 0.5% at 12 h. CONCLUSIONS--Detection of CCK gene expression in human duodenal biopsy specimens is feasible. Stimulation of CCK release after a meal is followed by an increase in CCK-mRNA in the duodenal mucosa.
Centre de Recherche en Nutrition Humaine de Nantes, CHU G.-et-R.-Laennec.
N
N
Bentouimou
V
V
Leray
F
F
Zerbib
H M
HM
Blottière
J P
JP
Galmiche
S
S
Bruley des Varannes
D000328:Adult; D015152:Blotting, Northern; D002766:Cholecystokinin / Q000032:analysis / Q000235:genetics*; D004386:Duodenum / Q000502:physiology*; D016099:Endoscopy, Gastrointestinal / Q000379:methods*; D005260:Female; D005527:Food, Fortified; D006801:Humans; D008055:Lipids; D008297:Male; D012333:RNA, Messenger / Q000032:analysis*; D012016:Reference Values; D014158:Transcription, Genetic
D008055:Lipids; D012333:RNA, Messenger; D002766:Cholecystokinin
Expression du gène de la cholécystokinine. Mise en évidence à partir de biopsies duodénales chez l'homme.