Immunohistochemical assessment of cell proliferation in plant tissues using formaldehyde-fixed paraffin-embedded material.
Cell proliferation in formaldehyde-fixed and wax-embedded sections of root tissues from Vicia faba beans was assessed by using a monoclonal antibody raised against the proliferating cell nuclear antigen (PCNA). Positive PCNA immunoreactivity was restricted to nuclei in known proliferating zones of the root. The immunoreactivity of cells in metaphase/anaphase of mitosis showed weaker PCNA immunoreactivity while nuclei in telophase showed no PCNA immunoreactivity indicating that this method is well suited to label proliferating cells in plant tissues.
Schumacher
U
U
University of Southampton, UK.
D000911:Antibodies, Monoclonal; D002455:Cell Division / Q000502:physiology; D002467:Cell Nucleus / Q000737:chemistry; D007887:Fabaceae / Q000737:chemistry; D005557:Formaldehyde; D007150:Immunohistochemistry; D016612:Paraffin Embedding; D018517:Plant Roots / Q000737:chemistry / Q000166:cytology*; D010946:Plants, Medicinal; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D016707:Tissue Fixation
D000911:Antibodies, Monoclonal; D018809:Proliferating Cell Nuclear Antigen; D005557:Formaldehyde