[Effect of the liposolubility of free radical scavengers on the production of antigen P24 from a HIV infected monocytic cell line].
A large body of evidence indicates that AIDS may be the consequence of a virus-induced antioxidant deficiency and implicates reactive oxygen species (ROS) in the pathogenesis of HIV infection. The high level of antigenic acid and cytokines activities in AIDS results in the production of superoxides (O2-), hydrogen peroxide (H202) and hydroxyl radicals (OH). HIV-infected T cells display low levels of SOD, catalase, thioredoxin and glutatione peroxidase rendering them susceptible to undergo apoptosis. Induction of NF kappa B and HIV replication are at least in part dependent on reactive oxygen intermediates. We examined the protective effects of two antioxidants. Ferulic Acid (FA) and Ethyl Ferulate (EF) at 1, 5, 10, 10 microM on the TNF induced HIV activation in the chronically infected promonocytic U1 cell line. FA and EF at 5 microM elicit a marked decrease of HIV p24 release. HIV inhibition was greater after pretreatment with EF than with FA. At these concentrations, no cytotoxicity was observed. When SOD (100 UI) was combined with EF and FA no more inhibition was observed. But when SOD was added alone, it induced a marked inhibition (30%). This class of drugs may present potential interest as antiviral agents or as adjuvant therapy in AIDS.
Laboratoire de Biochimie, Hôpital Antoine-Béclère, CLAMART.
M
M
Edeas
Y
Y
Khalfoun
Y
Y
Lazizi
L
L
Vergnes
S
S
Labidalle
E
E
Postaire
A
A
Lindenbaum
D000975:Antioxidants / Q000494:pharmacology*; D003373:Coumaric Acids / Q000494:pharmacology*; D004357:Drug Synergism; D016166:Free Radical Scavengers / Q000494:pharmacology*; D015488:HIV Antigens / Q000187:drug effects*; D016655:HIV Core Protein p24 / Q000276:immunology; D015658:HIV Infections / Q000276:immunology*; D006801:Humans; D050356:Lipid Metabolism; D009000:Monocytes / Q000276:immunology* / Q000821:virology*; D012995:Solubility; D013482:Superoxide Dismutase / Q000494:pharmacology; D014779:Virus Replication / Q000187:drug effects
D000975:Antioxidants; D003373:Coumaric Acids; D016166:Free Radical Scavengers; D015488:HIV Antigens; D016655:HIV Core Protein p24; C004999:ferulic acid; D013482:Superoxide Dismutase
Influence de la liposolubilité des molécules antiradicalaires sur la production d'antigène P24 par des cellules monocytaires infectées par le VIH.