Deregulated c-Fos/AP-1 accelerates cell cycle progression of B lymphocytes stimulated with lipopolysaccharide.
We implicated deregulated c-Fos/AP-1 in proliferative response of B lymphocytes stimulated with lipopolysaccharide (LPS) using splenic B cells from c-fos transgenic (Mx-c-fos) mice. Levels of DNA synthesis of the Mx-c-fos B cells were augmented in proportion to the amount of AP-1. Since the number of LPS-responding splenic B cells from Mx-c-fos mice was similar to that from control mice duplication time of the Mx-c-fos B cells (0.9 days) was much shorter than that of the control B cells (2.1 days), this augmentation is explained by the acceleration of cell cycle progression by deregulated c-Fos/AP-1. These results suggest that AP-1 is a major regulatory factor for cell cycle progression of B cells activated with LPS.
Phuchareon
J
J
Division of Developmental Genetics, Chiba University School of Medicine, Japan.
Tokuhisa
T
T
D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects* / Q000276:immunology; D002453:Cell Cycle / Q000187:drug effects / Q000276:immunology; D002455:Cell Division / Q000187:drug effects / Q000276:immunology; D005786:Gene Expression Regulation / Q000187:drug effects / Q000276:immunology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects*; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D016760:Proto-Oncogene Proteins c-fos / Q000187:drug effects / Q000235:genetics / Q000494:pharmacology*; D018808:Transcription Factor AP-1 / Q000187:drug effects / Q000235:genetics / Q000494:pharmacology*
D008070:Lipopolysaccharides; D016760:Proto-Oncogene Proteins c-fos; D018808:Transcription Factor AP-1