Direct sequencing of hepatitis A virus strains isolated during an epidemic in France.
Direct sequencing of PCR products was used to study the VP1 region of the hepatitis A virus (HAV) genome (position 2199 to 2356) of nine strains isolated from human stools collected during a hepatitis A epidemic (western France, 1992), three strains from environmental samples (1990, 1991, and 1992), and two HAV cell culture isolates (the French strain CF53/Lyon and strain CLF). These viruses differed from CF53/Lyon (genotype I) by between 1 and 10.3%, and results indicated the existence of two groups of strains belonging to two different subgenotypes (IA and IB). With this sequencing technique it was possible to monitor the epidemiology of HAV and study its relations.
Laboratoire de Virologie, Institut de Biologie, Nantes, France.
V
V
Apaire-Marchais
B H
BH
Robertson
V
V
Aubineau-Ferre
M G
MG
Le Roux
F
F
Leveque
L
L
Schwartzbrod
S
S
Billaudel
D000293:Adolescent; D000328:Adult; D000595:Amino Acid Sequence; D001483:Base Sequence; D002648:Child; D004279:DNA, Viral / Q000235:genetics; D004196:Disease Outbreaks; D005243:Feces / Q000821:virology; D005260:Female; D005602:France / Q000453:epidemiology; D014644:Genetic Variation; D005838:Genotype; D006506:Hepatitis A / Q000453:epidemiology* / Q000821:virology*; D006507:Hepatovirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D015678:Viral Structural Proteins / Q000235:genetics
D004279:DNA, Viral; C096539:VP1 protein, hepatitis A virus; D015678:Viral Structural Proteins