Constitutive increase of RecA protein: its influence on pyrimidine dimer excision and survival of UV-irradiated Escherichia coli.
Transformation of E. coli with the plasmid pRA containing recA gene increased the constitutive level of RecA protein 50-67 fold. This slightly inhibited pyrimidine dimer excision and reduced cell survival in three investigated, UV-irradiated E. coli strains. Our data support the view that RecA protein prematurely present at a high level may mask the dimers. The masking subsequently reduces the dimer excision and switches off the inducing signal.
Department of Molecular Genetics, Slovak Academy of Sciences, Bratislava, Slovakia.
V
V
Slezáriková
F
F
Masek
I
I
Fridrichová
M
M
Pirsel
M
M
Sedliaková
D004269:DNA, Bacterial / Q000528:radiation effects; D004790:Enzyme Induction; D004926:Escherichia coli / Q000235:genetics / Q000528:radiation effects; D015964:Gene Expression Regulation, Bacterial; D011740:Pyrimidine Dimers / Q000378:metabolism*; D011938:Rec A Recombinases / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D013014:SOS Response, Genetics / Q000235:genetics* / Q000502:physiology; D014169:Transformation, Bacterial; D014466:Ultraviolet Rays
D004269:DNA, Bacterial; D011740:Pyrimidine Dimers; D011938:Rec A Recombinases