Light and electron microscopical demonstration of the ouabain-sensitive, potassium-dependent p-nitrophenylphosphatase activity (K-NPPase) using a Ce-Mg-double capture technique.
The cerium-based method of Kobayashi et al. for the histochemical demonstration of K-NPPase activity was improved. Besides Ce3+ additionally Mg2+ ions as orthophosphate capture were employed (double capture technique). For light microscopical purposes the Mg-phosphate was converted into Ce-phosphate by treatment of the sections with Ce-citrate yielding higher quantity of reaction product. Unspecific background staining was eliminated by EGTA. In the electron microscope this technique brought about fine granular reaction products without diffusion artefacts.
Gliesing
M
M
Department of Histochemistry, Friedrich Schiller University, Jena, Germany.
Kalicharan
D
D
Halbhuber
K J
KJ
D009597:4-Nitrophenylphosphatase / Q000032:analysis* / Q000378:metabolism; D000818:Animals; D003106:Colon / Q000201:enzymology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005260:Female; D006651:Histocytochemistry / Q000379:methods; D007413:Intestinal Mucosa / Q000166:cytology / Q000201:enzymology / Q000648:ultrastructure; D007668:Kidney / Q000166:cytology / Q000201:enzymology* / Q000648:ultrastructure; D007684:Kidney Tubules / Q000201:enzymology / Q000648:ultrastructure; D008297:Male; D008854:Microscopy, Electron / Q000379:methods; D010042:Ouabain / Q000494:pharmacology; D051381:Rats; D013363:Submandibular Gland / Q000166:cytology / Q000201:enzymology* / Q000648:ultrastructure
D004791:Enzyme Inhibitors; D010042:Ouabain; D009597:4-Nitrophenylphosphatase