Phosphorylation of threonine 558 in the carboxyl-terminal actin-binding domain of moesin by thrombin activation of human platelets.
The phosphorylation and localization of the membrane-linking protein moesin was analyzed during early activation of platelets with thrombin. Activated platelets elaborate filopodia and spread to assume flat pancake-like shapes, and moesin is localized in filopodia and cell body. In resting platelets, approximately 25% of moesin molecules are phosphorylated as shown by metabolic labeling with 32P(i) and by isoelectric focusing. Within seconds after exposure to thrombin, phosphorylation increases, reaching a maximum of 35% labeled molecules by 1 min, followed by a decrease to a new basal level within 5 min. This modification affects a single residue, Thr558, which is located within or close to a binding site for F-actin. Rapid shifts (0-100%) in the number of phosphorylated molecules are observed in the presence of inhibitors of serine/threonine kinases and phosphatases. Inhibitors affecting tyrosine phosphorylation also modulate phosphorylation at this site suggesting that the enzymes involved in the modification of Thr558 are regulated by tyrosine phosphorylation. Platelets respond to both extremes of modification by forming extremely long filopodia and the absence of spreading on glass. Completely phosphorylated moesin is concentrated together with F-actin in the center of the cell. The rapid modification of moesin at or near its actin-binding domain suggests a model for regulated membrane-cytoskeleton interaction during cell activation.
Department of Pathology, Laboratory of Experimental Oncology, Stanford University School of Medicine, California 94305-5324, USA.
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Nakamura
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Amieva
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Furthmayr
D000199:Actins / Q000378:metabolism*; D000595:Amino Acid Sequence; D001704:Biopolymers; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism; D002460:Cell Line; D015180:Electrophoresis, Gel, Two-Dimensional; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006801:Humans; D007700:Kinetics; D008840:Microfilament Proteins; D008969:Molecular Sequence Data; D010749:Phosphoprotein Phosphatases / Q000037:antagonists & inhibitors; D010766:Phosphorylation; D015539:Platelet Activation; D011485:Protein Binding; D047428:Protein Kinase Inhibitors; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D013347:Subcellular Fractions / Q000378:metabolism; D013912:Threonine / Q000378:metabolism*; D013917:Thrombin / Q000494:pharmacology*
D000199:Actins; D001704:Biopolymers; D004791:Enzyme Inhibitors; D008840:Microfilament Proteins; D047428:Protein Kinase Inhibitors; D011506:Proteins; C071058:moesin; D013912:Threonine; D010749:Phosphoprotein Phosphatases; D013917:Thrombin