Immunological and partial sequence identity of mouse BM180 with wheat alpha-gliadin.
BM180, a novel 180-kDa basement membrane protein enriched in guanidine-HCl extracts of lacrimal and parotid exocrine secretory glands, was immunopurified using the secretion inhibitory monoclonal antibody 3E12. The N-terminal amino acid sequence was found to be VRVPVPQLQPQNP. An identical sequence comprises the N-terminus of the wheat storage protein alpha-gliadin. The presence of a gliadin-like protein in basement membranes was confirmed using a monoclonal and several polyclonal anti-gliadin antibodies, the former of which detected a 180-kDa protein in basement membrane blots. A full-length alpha-gliadin cDNA was found to hybridize at high stringency with mouse and human genomic DNA; and in lacrimal gland Northern blots with a 2.3-kb message. Since BM180 appears to be required for stimulus-secretion coupling by lacrimal acinar cells, circulating anti-alpha-gliadin antibodies associated with Sjögren's syndrome ('Dry Eye') and more commonly in Coeliac disease, may be secretion inhibitory.
Department of Cell Biology, University of Virginia, Charlottesville, USA.
G W
GW
Laurie
P J
PJ
Ciclitira
H J
HJ
Ellis
G
G
Pogany
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D001485:Basement Membrane / Q000737:chemistry*; D003429:Cross Reactions; D018076:DNA, Complementary / Q000235:genetics; D005903:Gliadin / Q000235:genetics* / Q000276:immunology*; D006801:Humans; D007120:Immunochemistry; D008565:Membrane Proteins / Q000235:genetics* / Q000276:immunology*; D051379:Mice; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D014908:Triticum
D000911:Antibodies, Monoclonal; D018076:DNA, Complementary; D008565:Membrane Proteins; D005903:Gliadin