A novel quantitative receptor autoradiography and in situ hybridization histochemistry technique using storage phosphor screen imaging.
A new technique of image acquisition for quantitative receptor autoradiography and in situ hybridization histochemistry was developed using storage phosphor screen imaging. This method was at least 4-5 times faster than conventional film densitometry. Two of the advantages of the phosphor screen method are high sensitivity and wide linear range of response. Other aspects of this method were compared with those of conventional densitometry. Use of storage phosphor screen imaging will allow greatly increased speed of pharmacological screening procedures that utilize quantitative autoradiography.
Department of Pharmacology and Toxicology, University of Mississippi Medical Center, Jackson 39216, USA.
T
T
Ito
T
T
Suzuki
D K
DK
Lim
S E
SE
Wellman
I K
IK
Ho
D000818:Animals; D001345:Autoradiography / Q000379:methods*; D001921:Brain / Q000033:anatomy & histology / Q000378:metabolism; D019086:Bridged Bicyclo Compounds, Heterocyclic; D002138:Calibration; D003720:Densitometry; D003838:Deoxyadenine Nucleotides / Q000378:metabolism; D006651:Histocytochemistry / Q000379:methods*; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D017403:In Situ Hybridization / Q000379:methods*; D008297:Male; D015345:Oligonucleotide Probes; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D011963:Receptors, GABA-A / Q000187:drug effects / Q000378:metabolism*
D019086:Bridged Bicyclo Compounds, Heterocyclic; D003838:Deoxyadenine Nucleotides; D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D011963:Receptors, GABA-A; C037476:tert-butylbicyclophosphorothionate; C026600:2'-deoxyadenosine triphosphate