A computer program for predicting recovery of SDS-protein in the automated HPGE-1000 apparatus.
The commercial automated gel electrophoresis apparatus (HPGE-1000 of LabIntelligence, Menlo Park, CA) allows one to recover the material migrating and visualized as a fluorescent-labeled band by electrophoresis into a collection cup located above the band at a right angle to the orientation of the separation path. The degree of recovery is a function of sample load (peak area), electrophoresis time at constant field strength, the mobility of the material and band width. Neglecting the latter, recovery of several SDS-proteins was measured as a function of the first three parameters. These measurements were used as a data base for a computer program capable of predicting, by interpolation of the experimental values, the time of electrophoresis needed to obtain a specified degree of recovery, or the degree of recovery obtained after a desired time of electrophoresis into the collection cup.
National Center for Research Resources, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892-1855, USA.
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Aldroubi
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Zakharov
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Chrambach
D001322:Autoanalysis / Q000295:instrumentation*; D003207:Conalbumin / Q000302:isolation & purification; D004587:Electrophoresis, Agar Gel / Q000295:instrumentation*; D007768:Lactalbumin / Q000302:isolation & purification; D010047:Ovalbumin / Q000302:isolation & purification; D010940:Plant Proteins / Q000302:isolation & purification; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification*; D012710:Serum Albumin, Bovine / Q000302:isolation & purification; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry*; D012984:Software; D014361:Trypsin Inhibitors; D000516:alpha-Amylases / Q000037:antagonists & inhibitors
D010940:Plant Proteins; D011506:Proteins; D014361:Trypsin Inhibitors; D003207:Conalbumin; D012710:Serum Albumin, Bovine; D012967:Sodium Dodecyl Sulfate; D010047:Ovalbumin; D007768:Lactalbumin; D000516:alpha-Amylases