snRNA interactions at 5' and 3' splice sites monitored by photoactivated crosslinking in yeast spliceosomes.
Splice site recognition and catalysis of the transesterification reactions in the spliceosome are accompanied by a dynamic series of interactions involving conserved or invariant sequences in the spliceosomal snRNAs. We have used site-specific photoactivated crosslinking in yeast spliceosomes to monitor interactions between snRNAs and exon sequences near the 5' and 3' splice sites. The last nucleotide of the 5' exon can be crosslinked to an invariant loop sequence in U5 SnRNA before and after 5' splice site cleavage. The first nucleotide of the 3' exon can also be crosslinked to the same U5 loop sequence, but this contact is only detectable after the first transesterification. These results are in close agreement with earlier data from mammalian splicing extracts, and they are consistent with a model in which U5 snRNA aligns the 5' and 3' exons for the second transesterification. After the first catalytic step of splicing, the first nucleotide of the 3' exon can also crosslink to nt U23 in U2 snRNA. This is one of a cluster of residues in U2-U6 helix I implicated by mutational analysis in the second catalytic step of splicing. The crosslinking data suggest that these residues in U2-U6 helix I are in close proximity to the scissile phosphodiester bond at the 3' splice site prior to the second transesterification. These results constitute the first biochemical evidence for a direct interaction between the 3' splice site and U2 snRNA.
MRC Laboratory of Molecular Biology, Cambridge, United Kingdom.
A J
AJ
Newman
S
S
Teigelkamp
J D
JD
Beggs
D001483:Base Sequence; D001710:Biotin / Q000737:chemistry; D017124:Conserved Sequence; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012322:RNA Precursors / Q000032:analysis / Q000737:chemistry; D012326:RNA Splicing; D012331:RNA, Fungal; D012333:RNA, Messenger / Q000138:chemical synthesis; D012342:RNA, Small Nuclear / Q000737:chemistry / Q000235:genetics*; D016914:Ribonuclease H; D017413:Ribonucleoprotein, U2 Small Nuclear / Q000032:analysis / Q000737:chemistry; D017415:Ribonucleoprotein, U5 Small Nuclear / Q000032:analysis / Q000737:chemistry; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D017381:Spliceosomes / Q000235:genetics*; D014466:Ultraviolet Rays
D012322:RNA Precursors; D012331:RNA, Fungal; D012333:RNA, Messenger; D012342:RNA, Small Nuclear; D017413:Ribonucleoprotein, U2 Small Nuclear; D017415:Ribonucleoprotein, U5 Small Nuclear; D001710:Biotin; D016914:Ribonuclease H