CD4 enumeration in HIV infected individuals.
In the present study, two alternative methods for the enumeration of PB CD4+ lymphocytes are compared with conventional flow cytometry (FCM): the TRAX CD4 tm enzyme immunoassay test kit and a new method using an internal biological standard (liophilized lymphocytes) for the direct enumeration of total PB CD4+ T-cells. Both methods showed an overall high degree of correlation with conventional FCM. However, the TRAxCD4 tm enzyme immunoassay showed a significant overestimation of PB CD4+ T-cells that was not only due to monocyte-derived CD4 molecules and the use of liophilized lymphocytes as an internal biological standard showed a low level of correlation for patients displaying low CD4 counts.
Lopez
A
A
Servicio General de Citometria, University of Salamanca, Spain.
Ciudad
J
J
Cordero
M
M
Orfao
A
A
D018791:CD4 Lymphocyte Count / Q000379:methods*; D002452:Cell Count / Q000379:methods; D005434:Flow Cytometry; D015658:HIV Infections / Q000097:blood*; D006801:Humans; D007124:Immunoenzyme Techniques