Inflammatory cell populations in bronchial biopsies from aspirin-sensitive asthmatic subjects.
The inflammatory cell infiltrate in bronchial biopsies of 12 aspirin-sensitive asthmatic (ASA) subjects and eight non-aspirin-sensitive (non-ASA) control subjects have been compared. Biopsies were taken from a right middle or lower lobe segmental carina using fiberoptic bronchoscopy. The biopsies were snap-frozen in OCT, and sections 5 microns thick were doubled immunostained using a rabbit polyclonal antibody to the enzyme 5-lipoxygenase (5-LO) and with a monoclonal antibody to neutrophils (NP57), macrophages (EMB11), and total (BMK13) and activated eosinophils (EG2), mast cells (AA1), and T-lymphocytes (anti-CD3). There was no significant difference in the total numbers of cells staining for 5-LO between the two groups of subjects. As a percentage of total 5-LO cells, there were significantly more mast cells (12.9 +/- 3.8% versus 3.4 +/- 3.1%; p = 0.039) and total eosinophils (34.7 +/- 9.4% versus 11.1 +/- 3.8%; p = 0.044) and significantly fewer macrophages (23.3 +/- 6.1% versus 39.8% +/- 5.3; p = 0.041) in the bronchial biopsies from ASA subjects as compared with non-ASA patients. The numbers of neutrophils, T-lymphocytes, and activated eosinophils were similar for the two groups. The increased numbers of eosinophils and mast cells identified in the bronchial tissue from aspirin-sensitive asthmatic subjects may be the source of the enhanced cysteinyl leukotriene production observed in these subjects.
Department of Allergy and Respiratory Medicine, UMDS, Guy's Hospital, London, United Kingdom.
S M
SM
Nasser
R
R
Pfister
P E
PE
Christie
A R
AR
Sousa
J
J
Barker
M
M
Schmitz-Schumann
T H
TH
Lee
D000280:Administration, Inhalation; D000284:Administration, Oral; D000287:Administration, Topical; D000305:Adrenal Cortex Hormones / Q000008:administration & dosage; D000328:Adult; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage; D001241:Aspirin / Q000009:adverse effects*; D001249:Asthma / Q000188:drug therapy / Q000473:pathology*; D001706:Biopsy; D001980:Bronchi / Q000166:cytology* / Q000473:pathology; D001993:Bronchodilator Agents / Q000008:administration & dosage; D001999:Bronchoscopy; D019819:Budesonide; D002452:Cell Count; D004396:Coloring Agents; D003627:Data Interpretation, Statistical; D004342:Drug Hypersensitivity / Q000209:etiology*; D004804:Eosinophils / Q000166:cytology; D005260:Female; D005938:Glucocorticoids; D006801:Humans; D007150:Immunohistochemistry; D007249:Inflammation / Q000473:pathology*; D008264:Macrophages / Q000166:cytology; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000166:cytology; D011239:Prednisolone / Q000008:administration & dosage; D011282:Pregnenediones / Q000008:administration & dosage; D011817:Rabbits; D013601:T-Lymphocytes / Q000166:cytology
D000305:Adrenal Cortex Hormones; D000893:Anti-Inflammatory Agents; D001993:Bronchodilator Agents; D004396:Coloring Agents; D005938:Glucocorticoids; D011282:Pregnenediones; D019819:Budesonide; D011239:Prednisolone; D001241:Aspirin