Chemoprevention of mammary preneoplasia. In vitro effects of a green tea polyphenol.
The major findings of this study can be summarized as follows: (1) the c-myc oncogene-transfected and MTV-expressing mammary epithelial cells exhibit aberrant hyperproliferation in vitro preceding tumorigenesis in vivo; (2) upregulation of aberrant hyperproliferation (i.e., anchorage-independent growth) in initiated cells represents a cellular marker for preneoplastic transformation; (3) the tea polyphenol EGCG differentially downregulates aberrant hyperproliferation in myc oncogene- and MTV-initiated cells; (4) the present in vitro model provides an efficient assay for chemoprevention of mammary preneoplasia by naturally occurring compounds.
Division of Carcinogenesis and Prevention, Strang-Cornell Cancer Research Laboratory, Cornell University Medical College, New York, New York, USA.
R
R
Araki
S
S
Inoue
M P
MP
Osborne
N T
NT
Telang
D000818:Animals; D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D002448:Cell Adhesion; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000187:drug effects*; D002478:Cells, Cultured; D005260:Female; D016259:Genes, myc; D008321:Mammary Glands, Animal; D008325:Mammary Neoplasms, Experimental / Q000517:prevention & control*; D051379:Mice; D008807:Mice, Inbred BALB C; D011230:Precancerous Conditions / Q000517:prevention & control*; D013662:Tea; D014162:Transfection
D016588:Anticarcinogenic Agents; D013662:Tea; D002392:Catechin; C045651:epigallocatechin gallate