Relationship between airway eosinophilia and airway hyperresponsiveness in a late asthmatic model of guinea pigs.
To elucidate the mechanism of development of asthma, we tried to develop a model which elicited a late asthmatic response by a combination of systemic and inhaled sensitization with ovalbumin in guinea pigs. Eighty-seven percent of animals elicited both an immediate and late asthmatic response after the third antigen inhalation. Airway eosinophilia and airway hyperresponsiveness (AHR) induced after the third challenge were more severe than those after the first challenge. There was a good correlation between airway eosinophilia and AHR in this model under experimental modulation of the number of eosinophils, such as by interleukin 5 or antieosinophil antibody injection. These results demonstrate that eosinophils play an important role in the development of late asthmatic response and AHR.
Biological Research Laboratories, Sankyo Co., Ltd., Tokyo, Japan.
K
K
Itoh
E
E
Takahashi
O
O
Mukaiyama
Y
Y
Satoh
T
T
Yamaguchi
D000818:Animals; D000906:Antibodies / Q000097:blood; D001249:Asthma / Q000209:etiology / Q000503:physiopathology*; D016535:Bronchial Hyperreactivity / Q000209:etiology / Q000503:physiopathology*; D001985:Bronchial Provocation Tests; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry / Q000166:cytology; D016085:Bronchoconstrictor Agents; D004195:Disease Models, Animal; D004804:Eosinophils / Q000502:physiology*; D006168:Guinea Pigs; D006632:Histamine / Q000032:analysis; D015848:Interleukin-5 / Q000276:immunology; D007958:Leukocyte Count; D017997:Leukotriene C4 / Q000032:analysis; D008297:Male; D016210:Methacholine Chloride; D010047:Ovalbumin / Q000276:immunology; D010323:Passive Cutaneous Anaphylaxis / Q000276:immunology; D011657:Pulmonary Eosinophilia / Q000209:etiology / Q000503:physiopathology*; D013929:Thromboxane B2 / Q000032:analysis; D013997:Time Factors
D000906:Antibodies; D016085:Bronchoconstrictor Agents; D015848:Interleukin-5; D016210:Methacholine Chloride; D017997:Leukotriene C4; D013929:Thromboxane B2; D006632:Histamine; D010047:Ovalbumin