A new method of bipolymerase sequencing prevents "stop-bands".
This article presents a relatively quick and cost-effective DNA sequencing method that prevents the formation of stop-bands. This method uses a combination of Taq and Sequenase that allows sequencing at both low and high temperatures. The ability to sequence at a high temperature appears to be the fundamental component in preventing stop-band formation in G+C rich regions.
Austin
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Department of Zoology, University of Texas, Austin 78712, USA.
D016477:Artifacts; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D004259:DNA-Directed DNA Polymerase / Q000737:chemistry* / Q000378:metabolism; D017422:Sequence Analysis, DNA / Q000379:methods*; D019914:Taq Polymerase
D004247:DNA; D019914:Taq Polymerase; C034970:bacteriophage T7 induced DNA polymerase; D004259:DNA-Directed DNA Polymerase