New types of large-pore polyacrylamide-agarose mixed-bed matrices for DNA electrophoresis: pore size estimation from Ferguson plots of DNA fragments.
The average pore size value of gels containing polyacrylamide, covalently linked to agarose, was found to be 30% higher than the value of a regular N,N'-methylenebisacrylamide (Bis) cross-linked gel of the same %T. By increasing the agarose concentration (10% of the total amount of polyacrylamide), gels containing low amounts of acrylamide (1.5-2%) are reproducibly obtained; their pore sizes are 130% larger than the pore sizes of a 4%T, 3.3%C polyacrylamide gel. In general, mixed-bed matrices were found to be more elastic and mechanically stronger than classical polyacrylamide gels since an agarose-induced gelation process takes place during their polymerization.
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Istituto di Chimica degli Ormoni, CNR, Milano, Italy.
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D000180:Acrylic Resins / Q000737:chemistry; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004247:DNA / Q000032:analysis*; D004587:Electrophoresis, Agar Gel / Q000295:instrumentation / Q000379:methods* / Q000706:statistics & numerical data; D004591:Electrophoresis, Polyacrylamide Gel / Q000295:instrumentation / Q000379:methods* / Q000706:statistics & numerical data; D008970:Molecular Weight; D010957:Plasmids; D011108:Polymers / Q000737:chemistry; D012685:Sepharose / Q000737:chemistry
D000180:Acrylic Resins; D011108:Polymers; C016680:polyacrylamide gels; D004247:DNA; D012685:Sepharose