Structural characteristics of peptidoglycan fragments required to prime mice for induction of anaphylactoid reactions by lipopolysaccharides.
Structural characteristics of peptidoglycan fragments required to prime mice for the induction of anaphylactoid reactions by Salmonella abortusequi lipopolysaccharide were examined in endotoxin-resistant C3H/HeJ mice, with special focus on the disaccharide-pentapeptide [N-acetylglucosaminyl-beta(1-4)-N-acetylmuramyl-L-alanyl-D -isoglutaminyl-meso-2,6-diaminopimelyl (DAP)-D-alanyl-D -alanine] and its smaller partial derivatives. The bacterial and synthetic muramyl tripeptides (DAP- and lysine [Lys]-type, respectively) and synthetic muramyl dipeptide primed mice for induction of anaphylactoid reactions accompanied by death within 1 h. The disaccharide-tripeptide exhibited weaker activity, and the disaccharide-tetrapeptide and muramyl tetrapeptide exhibited marginal activity. In contrast, intact peptidoglycans of various bacteria and the disaccharide-pentapeptide lacked the priming activity, although they showed adjuvant activity similar to that of the above components.
Takada
H
H
Department of Microbiology and Immunology, Kagoshima University Dental School, Japan.
Kawabata
Y
Y
Kawata
S
S
Kusumoto
S
S
D000707:Anaphylaxis / Q000209:etiology*; D000818:Animals; D005260:Female; D008070:Lipopolysaccharides / Q000276:immunology*; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D010457:Peptidoglycan / Q000737:chemistry / Q000276:immunology*; D013329:Structure-Activity Relationship
D008070:Lipopolysaccharides; D010457:Peptidoglycan