Resistance to beta-lactam antibiotics and beta-lactamase production of Bacteroides, Porphyromonas and Prevotella strains.
Resistance to beta-lactam antibiotics of 183 clinical isolates belonging to Bacteroides, Porphyromonas and Prevotella was tested by a micro-broth dilution MIC method. Beta-lactamase production was screened by using nitrocefin sticks. Prevalence of resistance to different beta-lactam antibiotics was higher among the Bacteroides strains other than B. fragilis parallel with a beta-lactamase production (88% and 96%, respectively). Resistance was observed less frequently among Porphyromonas and Prevotella strains. Cefoxitin resistance was 11.5% and amoxicillin/clavulanic acid 3.5% among Bacteroides isolates, whereas no resistance was found to these antibiotics among the Porphyromonas and Prevotella strains. All strains tested were susceptible for imipenem. Beta-lactamase production of selected isolates was tested quantitatively. Beta-lactamase of B. fragilis 1 and B. levii differed in their isoelectric points, substrate profiles and inhibition by clavulanic acid, sulbactam and tazobactam.
Institute of Clinical Microbiology, Albert Szent-Györgyi University Medical School, Szeged, Hungary.
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Nagy
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Szóke
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Gacs
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Csiszár
D000900:Anti-Bacterial Agents / Q000378:metabolism / Q000494:pharmacology*; D001439:Bacteroides / Q000187:drug effects* / Q000201:enzymology; D004352:Drug Resistance, Microbial; D017861:Porphyromonas / Q000187:drug effects* / Q000201:enzymology; D018720:Prevotella / Q000187:drug effects* / Q000201:enzymology; D001618:beta-Lactamases / Q000096:biosynthesis*; D047090:beta-Lactams
D000900:Anti-Bacterial Agents; D047090:beta-Lactams; D001618:beta-Lactamases