Role of LFA-1/ICAM-1-dependent cell adhesion in CD40-mediated inhibition of anti-IgM antibody-induced B-cell death.
Cross-linking of surface IgM by anti-IgM antibody caused activation-induced cell death of a surface IgM+, IgD+ human B lymphoma cell line, B104. The dying B104 cells did not show the morphology of apoptosis but did show that of necrosis. However, anti-IgM antibody caused apoptosis of another surface IgM+, IgD+ human B lymphoma cell line, DND-39. The influx of extracellular Ca2+ was necessary for the cell deaths of B104 and DND-39 caused by anti-IgM antibody. Their cell deaths were inhibited by cyclosporine. The anti-IgM antibody-induced cell death of DND-39, but not that of B104, was prevented by costimulation with anti-CD40 antibody. In human peripheral blood B-cells, anti-IgM antibody inhibited cell cycle transition induced by Staphylococcus aureus Cowan I at the G2/M interphase without inhibition of DNA synthesis. In this system, too, anti-CD40 antibody canceled the inhibitory signal transduced through surface IgM and increased the number of M phase cells. Blocking antibodies against the leukocyte function-associated antigen-I/intercellular adhesion molecule-1 system decreased the rescue effect of anti-CD40 antibody in both DND-39 cells and peripheral B-cells, which shows that leukocyte function-associated antigen-1/intercellular adhesion molecule-1-dependent cell adhesion plays an important role in the CD40-mediated inhibition of surface IgM-mediated negative signals.
Department of Pediatrics, Kyoto University Hospital, Japan.
M
M
Mayumi
S
S
Sumimoto
Y
Y
Ohshima
K
K
Katamura
T
T
Heike
D
D
Hata
S
S
Kanazashi
K
K
Furusho
D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D000888:Antibodies, Anti-Idiotypic / Q000378:metabolism / Q000494:pharmacology*; D017209:Apoptosis / Q000502:physiology; D001402:B-Lymphocytes / Q000166:cytology* / Q000187:drug effects*; D001483:Base Sequence; D019013:CD40 Antigens / Q000276:immunology / Q000502:physiology*; D002448:Cell Adhesion / Q000502:physiology; D016923:Cell Death / Q000187:drug effects; D003429:Cross Reactions; D006801:Humans; D007072:Immunoglobulin D / Q000378:metabolism; D007075:Immunoglobulin M / Q000378:metabolism; D018799:Intercellular Adhesion Molecule-1 / Q000276:immunology / Q000502:physiology*; D016169:Lymphocyte Function-Associated Antigen-1 / Q000276:immunology / Q000502:physiology*; D016393:Lymphoma, B-Cell; D051379:Mice; D008969:Molecular Sequence Data; D009336:Necrosis / Q000503:physiopathology; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology; D013268:Stimulation, Chemical; D014407:Tumor Cells, Cultured
D000906:Antibodies; D000888:Antibodies, Anti-Idiotypic; D019013:CD40 Antigens; D007072:Immunoglobulin D; D007075:Immunoglobulin M; D016169:Lymphocyte Function-Associated Antigen-1; C044203:anti-IgM; D018799:Intercellular Adhesion Molecule-1