Use of somatic gene transfer to study lipoprotein metabolism in experimental animals in vivo.
Genetic manipulations of experimental animals have provided valuable information on lipoprotein metabolism in vivo. Somatic gene transfer is a novel method for introducing foreign genes into animals in vivo. Adenovirus-mediated transfer of genes for the LDL receptor, the receptor-associated protein, apolipoprotein A-I, apolipoprotein E, cholesterol 7 alpha-hydroxylase, and apolipoprotein B messenger RNA editing protein has resulted in high-level expression of these transgenes in mice and hamsters, and the production of interesting animal models of lipoprotein metabolism. Somatic gene transfer is a valuable tool for analyzing the role of transgenes in lipoprotein metabolism in vivo and for exploring the potential use of specific transgenes for human gene therapy.
Department of Cell Biology, Baylor College of Medicine, Houston, Texas, USA.
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D000071479:APOBEC-1 Deaminase; D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D001053:Apolipoproteins / Q000235:genetics; D002790:Cholesterol 7-alpha-Hydroxylase / Q000235:genetics; D006224:Cricetinae; D003564:Cytidine Deaminase / Q000235:genetics; D018014:Gene Transfer Techniques; D026541:Heymann Nephritis Antigenic Complex; D008074:Lipoproteins / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000235:genetics; D051379:Mice; D011973:Receptors, LDL / Q000235:genetics; D000098830:AICDA (Activation-Induced Cytidine Deaminase)
D001053:Apolipoproteins; D026541:Heymann Nephritis Antigenic Complex; D008074:Lipoproteins; D008562:Membrane Glycoproteins; D011973:Receptors, LDL; D002790:Cholesterol 7-alpha-Hydroxylase; D000098830:AICDA (Activation-Induced Cytidine Deaminase); D000071479:APOBEC-1 Deaminase; C000605089:APOBEC1 protein, human; C000605090:Apobec1 protein, mouse; D003564:Cytidine Deaminase