Bioconversion of 2,4-diamino-6-nitrotoluene to a novel metabolite under anoxic and aerobic conditions.
Under nitrate-reducing, nongrowth conditions, a Pseudomonas fluorescens species reduced 2,4,6-trinitrotoluene to aminodinitrotoluenes, which were then further reduced to diaminonitrotoluenes. 2,4-Diamino-6-nitrotoluene (2,4-DANT) was further transformed to a novel metabolite, 4-N-acetylamino-2-amino-6-nitrotoluene (4-N-AcANT), while 2,6-diamino-4-nitrotoluene (2,6-DANT) was persistent. Efforts to further degrade 2,4-DANT and 2,6-DANT under aerobic, nitrogen-limited conditions were unsuccessful; 2,6-DANT remained persistent, and 2,4-DANT was again transformed to the 4-N-AcANT compound.
Gilcrease
P C
PC
Department of Chemical and Bioresource Engineering, Colorado State University, Fort Collins 80523, USA.
Murphy
V G
VG
D000332:Aerobiosis; D000693:Anaerobiosis; D011551:Pseudomonas fluorescens / Q000378:metabolism*; D014052:Toluidines / Q000378:metabolism*
D014052:Toluidines; C046358:2,4-diamino-6-nitrotoluene