Genetic polymorphism of the H1 subunit of inter-alpha-trypsin inhibitor.
Inter-alpha-trypsin inhibitor (ITI) consists of 3 subunits (H1, H2 and L) which are encoded by 3 distinct genes. We attempted to prepare the subunit-specific monoclonal antibodies to identify the subunit carrying ITI polymorphism. Three monoclonal antibodies which were specific against each of these 3 subunits (ITI H1, ITI H2 and ITI L) were selected. ITI types could be detected by anti ITI H1 antibody, but not by anti ITI H2 or anti ITI L antibodies. From the result, it was proved that ITI polymorphism originates from the ITI H1 gene products, therefore we propose to call this system ITI H1 polymorphism.
Department of Forensic Medicine, Yamagata University School of Medicine, Japan.
A
A
Harada
K
K
Umetsu
T
T
Suzuki
D000510:Alpha-Globulins / Q000235:genetics*; D000818:Animals; D000911:Antibodies, Monoclonal; D015153:Blotting, Western; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D010641:Phenotype; D011110:Polymorphism, Genetic
D000510:Alpha-Globulins; D000911:Antibodies, Monoclonal; C011877:inter-alpha-inhibitor