Cloning and characterisation of the rabbit 5-HT1D alpha and 5-HT1D beta receptors.
The genes encoding the rabbit 5HT1D alpha and 5HT1D beta receptors have been cloned. The deduced amino acid sequence of these receptors shows 91-92% amino acid sequence identity with their human homologues, and similar high sequence identity with homologues from other species. The receptors were transiently expressed in COS-7 cells and exhibit a pharmacological profile closely resembling their human homologues, including a higher affinity of ketanserin for the 5-HT1D alpha subtype. However, sumatriptan had a lower affinity for both the rabbit receptors compared to their human counterparts. This may be accounted for by differences between the primary amino acid sequences of these species homologues.
Harwood
G
G
Receptor Pharmacology Research Groups, Wellcome Research Laboratories, Beckenham, Kent, UK.
Lockyer
M
M
Giles
H
H
Fairweather
N
N
D000595:Amino Acid Sequence; D000818:Animals; D001667:Binding, Competitive; D002460:Cell Line; D003001:Cloning, Molecular; D006801:Humans; D007650:Ketanserin / Q000378:metabolism; D008969:Molecular Sequence Data; D011817:Rabbits; D044302:Receptor, Serotonin, 5-HT1B; D044346:Receptor, Serotonin, 5-HT1D; D011985:Receptors, Serotonin / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D011994:Recombinant Proteins; D017385:Sequence Homology; D014162:Transfection
D044302:Receptor, Serotonin, 5-HT1B; D044346:Receptor, Serotonin, 5-HT1D; D011985:Receptors, Serotonin; D011994:Recombinant Proteins; D007650:Ketanserin