Existence and co-existence of vasoactive substances in nerve fibres supplying the abdomino-pelvic arterial tree of the female pig and cow.
The occurrence and co-localization of several presumed vasoactive neuropeptides, serotonin, and catecholamine-synthesising enzymes--tyrosine hydroxylase (TH), dopamine-beta-hydroxylase (D beta H) and phenylethanolamine-N-methyltransferase (PNMT)--were investigated in perivascular nerves supplying the systemic and distributing arteries of the abdomino-pelvic arterial tree of the female pig and certain arteries supplying female reproductive organs in the cow. As revealed by single immunofluorescence, perivascular axons immunoreactive for TH, D beta H, neuropeptide Y (NPY), vasoactive intestinal polypeptide (VIP), substance P (SP), calcitonin gene-related peptide (CGRP) and Leu-enkephalin (LENK) occurred in both species examined, whereas galanin-immunoreactive (GAL-IR) nerve fibres were found exclusively in the pig. PNMT-, serotonin-, dynorphin A-, alpha-neoendorphin-, bombesin- or cholecystokinin-IR nerve terminals were not observed. The following classes of perivascular nerve fibres might be distinguished in the present study: 1) noradrenergic (i.e. TH/D beta H-IR), 2) NPY-, 3) GAL- (pig only), 4) LENK-, 5) VIP-, 6) SP-, 7) VIP/NPY-, 8) SP/CGRP-, 9) SP/GAL- (pig only), 10) SP/VIP- (cow only), 11) TH/D beta H/NPY- and 12) TH/D beta H/NPY/LENK-IR. Distinct differences in the distribution of LENK- and SP-IR axons around particular parts of the studied arterial tree in individual species were also observed. The present data indicate that the abdomino-pelvic arterial tree of the pig and cow receive perivascular nerve fibres that exhibit diverse chemical codes, and that different chemical coding of perivascular nerve fibres in individual species may depend on the target organ of the particular artery.
Majewski
M
M
Department of Animal Anatomy, Faculty of Veterinary Medicine, Agricultural and Technical University of Olsztyn, Poland.
Kaleczyc
J
J
Sienkiewicz
W
W
Lakomy
M
M
D000005:Abdomen / Q000098:blood supply*; D000818:Animals; D001158:Arteries / Q000201:enzymology / Q000294:innervation; D002417:Cattle; D004299:Dopamine beta-Hydroxylase / Q000032:analysis; D005260:Female; D005455:Fluorescent Antibody Technique; D005836:Genitalia, Female / Q000098:blood supply; D009412:Nerve Fibers / Q000737:chemistry*; D009479:Neuropeptides / Q000032:analysis*; D010388:Pelvis / Q000098:blood supply*; D010625:Phenylethanolamine N-Methyltransferase / Q000032:analysis; D012701:Serotonin / Q000032:analysis; D013552:Swine; D014446:Tyrosine 3-Monooxygenase / Q000032:analysis
D009479:Neuropeptides; D012701:Serotonin; D014446:Tyrosine 3-Monooxygenase; D004299:Dopamine beta-Hydroxylase; D010625:Phenylethanolamine N-Methyltransferase