Light and electron microscopical demonstration of the ouabain-sensitive, potassium-dependent p-nitrophenylphosphatase activity (K-NPPase) using a Ce-Mg-double capture technique.
The cerium-based method of Kobayashi et al. for the histochemical demonstration of K-NPPase activity was improved. Besides Ce3+ additionally Mg2+ ions as orthophosphate capture were employed (double capture technique). For light microscopical purposes the Mg-phosphate was converted into Ce-phosphate by treatment of the sections with Ce-citrate yielding higher quantity of reaction product. Unspecific background staining was eliminated by EGTA. In the electron microscope this technique brought about fine granular reaction products without diffusion artefacts.
Department of Histochemistry, Friedrich Schiller University, Jena, Germany.
M
M
Gliesing
D
D
Kalicharan
K J
KJ
Halbhuber
D009597:4-Nitrophenylphosphatase / Q000032:analysis* / Q000378:metabolism; D000818:Animals; D003106:Colon / Q000201:enzymology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005260:Female; D006651:Histocytochemistry / Q000379:methods; D007413:Intestinal Mucosa / Q000166:cytology / Q000201:enzymology / Q000648:ultrastructure; D007668:Kidney / Q000166:cytology / Q000201:enzymology* / Q000648:ultrastructure; D007684:Kidney Tubules / Q000201:enzymology / Q000648:ultrastructure; D008297:Male; D008854:Microscopy, Electron / Q000379:methods; D010042:Ouabain / Q000494:pharmacology; D051381:Rats; D013363:Submandibular Gland / Q000166:cytology / Q000201:enzymology* / Q000648:ultrastructure
D004791:Enzyme Inhibitors; D010042:Ouabain; D009597:4-Nitrophenylphosphatase