Down-regulation of glial glutamate transporters after glutamatergic denervation in the rat brain.
Membrane-localized transporter proteins, expressed in both neurons and glial cells, are responsible for removal of extracellular glutamate in the mammalian CNS. The amounts and activities of these transporters may be under regulatory control. We demonstrate here that cortical lesions, which decrease striatal glutamate uptake in synaptosome-containing homogenates by approximately 50%, also decrease the striatal concentrations of the astrocytic glutamate transporter proteins, GLT-1 and GLAST by approximately 20-30%. Since GABA uptake activity was not decreased and glial fibrillary acidic protein was increased in the same samples, the lesion-induced losses of GLT-1 and GLAST were not caused by a general impairment of neuronal or glial function. The observed reduction in the two astrocytic glutamate transporters after corticostriatal nerve terminal degeneration indicates that their levels of expression are dependent on glutamatergic innervation.
Anatomical Institute, University of Oslo, Norway.
L M
LM
Levy
K P
KP
Lehre
S I
SI
Walaas
J
J
Storm-Mathisen
N C
NC
Danbolt
D018528:ATP-Binding Cassette Transporters / Q000502:physiology*; D027322:Amino Acid Transport System X-AG; D000818:Animals; D000906:Antibodies; D001253:Astrocytes / Q000502:physiology; D015536:Down-Regulation; D004586:Electrophoresis; D007150:Immunohistochemistry; D009457:Neuroglia / Q000502:physiology*; D009474:Neurons / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar
D018528:ATP-Binding Cassette Transporters; D027322:Amino Acid Transport System X-AG; D000906:Antibodies