Short-term postischemic hypoperfusion improves recovery of protein synthesis in the rat brain cortex.
A cell-free system from rat brain cortex was used to follow changes in protein synthesis after ischemia and reperfusion (four-vessel occlusion). The experiment was focused to prevent a violent burst of free oxygen radicals creation during the first period of postischemic reperfusion by short-term hypoperfusion. After 30 min of ischemia, the authors applied hypoperfusion produced by releasing one (right) carotid for the first 5 min of reperfusion lasting from 30 min to 3 d. Results obtained by this procedure show that the activity of protein synthesis machinery from hypoperfused brains is higher than normovolemic ones; the left hemisphere, which is contralateral to direct blood flow during hypoperfusion, shows better results than the right hemisphere.
Burda
J
J
Institute of Neurobiology, Slovak Academy of Sciences, Kosice, Slovak Republic.
Gottlieb
M
M
Vanický
I
I
Chavko
M
M
Marsala
J
J
D000818:Animals; D001923:Brain Chemistry / Q000502:physiology*; D002545:Brain Ischemia / Q000378:metabolism / Q000503:physiopathology*; D002474:Cell-Free System; D002540:Cerebral Cortex / Q000378:metabolism / Q000503:physiopathology*; D002560:Cerebrovascular Circulation / Q000502:physiology*; D007839:Functional Laterality; D008297:Male; D008928:Mitochondria / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000096:biosynthesis*; D010101:Oxygen Consumption / Q000502:physiology; D014176:Protein Biosynthesis; D051381:Rats; D017208:Rats, Wistar; D015424:Reperfusion; D016897:Respiratory Burst / Q000502:physiology
D009419:Nerve Tissue Proteins