Flow cytometry of breast tumors. Relevance to clinicopathology and survival.
Nuclear DNA content and cell cycle distribution in fresh tissues from 40 malignant and 10 benign breast tumors were assessed by flow cytometry using a DNA-specific fluorochrome, 4,6-diamidino-2phenyl-indole hydrochloride. DNA indices (DIs) (relative DNA content of tumor cells with reference to normal cells, 4,6-diamino-2phenyl-indole-hydrochloride ranged from 0.85 to 5.6 for malignant tumors and from 1.8 to 2.2 for benign tumors. Proliferating fraction (PF) (total cells in S and G2 + M phases) values were significantly higher in malignant tumors (35.4 +/- 14.75, mean +/- SD; P < .001) than in benign tumors (14.2 + 4.9) and adjacent normal tissues (6.6 + 2.73). DIs and cell cycle distribution correlated with clinicopathologic parameters, disease progression and survival. Four-year survival was greater in patients with a DI value of 1.8-2.2 as well as with SF values less than 10%.
Goud
T M
TM
Department of Pathology, Kidwai Memorial Institute of Oncology, Bangalore, Karnataka, India.
Dwarakanath
B S
BS
Augustus
M
M
Bhargava
K M
KM
D001943:Breast Neoplasms / Q000401:mortality / Q000473:pathology*; D002455:Cell Division; D004273:DNA, Neoplasm / Q000032:analysis*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D009367:Neoplasm Staging; D011003:Ploidies; D015996:Survival Rate
D004273:DNA, Neoplasm