Detection of maize DNA sequences amplified in wheat.
Genomic in situ hybridization to somatic metaphase chromosomes of hexaploid wheat cv. Chinese Spring using biotinylated maize genomic DNA as a probe revealed the existence of amplified maize DNA sequences in five pairs of chromosomes. The in situ hybridization sites were located on chromosomes 1A, 7A, 2B, 3B, and 7B. One pair of in situ hybridization sites was also observed in hexaploid oat. The locations and sizes of in situ hybridization sites varied among progenitor species.
Department of Plant Pathology, Kansas State University, Manhattan 66506-5502, USA.
J
J
Zhang
B
B
Friebe
B S
BS
Gill
D002874:Chromosome Mapping; D018744:DNA, Plant; D005784:Gene Amplification; D017403:In Situ Hybridization; D014908:Triticum / Q000235:genetics*; D003313:Zea mays / Q000235:genetics*
D018744:DNA, Plant