Neutralizing and infection-enhancing antibody responses to human immunodeficiency virus type 1 in long-term nonprogressors.
Serum antibodies from human immunodeficiency virus type 1 (HIV-1)-infected long-term non-progressors (LTNPs) and non-LTNPs were evaluated for virus neutralization and infection enhancement in vitro. Sera from LTNPs had higher average titers of neutralizing antibodies to HIV-1 strains IIIB and MN and more frequently neutralized primary isolates from progressors (14.9% vs. 1.3%, P = .002). Replication-competent HIV-1 was isolated from peripheral blood mononuclear cells and lymph nodes of 3 LTNPs. All viruses from LTNPs had a non-syncytium-inducing phenotype, were resistant to neutralization by autologous serum obtained at the time of virus isolation, and showed little evidence of a heightened sensitivity to neutralization by heterologous sera. Complement-mediated, antibody-dependent enhancement (C'-ADE) of HIV-1IIIB and primary isolates was equally prevalent for sera from LTNPs and non-LTNPs. Results indicate that LTNPs produce vigorous serum antibody responses and that long-term nonprogression is not associated with homologous neutralization or the absence of C'-ADE.
Montefiori
D C
DC
Department of Surgery, Duke University Medical Center, Durham, North Carolina 27710, USA.
Pantaleo
G
G
Fink
L M
LM
Zhou
J T
JT
Zhou
J Y
JY
Bilska
M
M
Miralles
G D
GD
Fauci
A S
AS
D000328:Adult; D018791:CD4 Lymphocyte Count; D015938:Complement Membrane Attack Complex / Q000276:immunology; D003602:Cytotoxicity, Immunologic / Q000276:immunology; D018450:Disease Progression; D005260:Female; D015483:HIV Antibodies / Q000032:analysis*; D015488:HIV Antigens / Q000276:immunology; D015658:HIV Infections / Q000276:immunology*; D006679:HIV Seropositivity / Q000276:immunology; D015497:HIV-1 / Q000276:immunology* / Q000302:isolation & purification; D006801:Humans; D016130:Immunophenotyping; D007963:Leukocytes, Mononuclear / Q000821:virology; D008198:Lymph Nodes / Q000821:virology; D008297:Male; D008875:Middle Aged; D009500:Neutralization Tests
D015938:Complement Membrane Attack Complex; D015483:HIV Antibodies; D015488:HIV Antigens