Automated high-performance liquid chromatographic method for the determination of a neuraminidase inhibitor (GG167) in human serum by pre-column fluorescence derivatisation using benzoin.
A pre-column fluorescence derivatisation high-performance liquid chromatographic method for the analysis of a neuraminidase inhibitor, GG167, in human serum is described. GG167 was extracted from serum samples using Bond Elut SCX solid-phase extraction cartridges, followed by derivatisation with benzoin prior to reversed-phase chromatography with fluorescence detection. This method has been automated using a Zymark robot and used in the analysis of human serum samples from clinical studies. The method has been shown to be valid over a concentration range of 10-800 ng/ml using a 1-ml sample volume.
Glaxo Research and Development Ltd, Greenford, Middlesex, UK.
R J
RJ
Stubbs
A J
AJ
Harker
D001322:Autoanalysis; D001573:Benzoin; D002498:Centrifugation; D002851:Chromatography, High Pressure Liquid; D004791:Enzyme Inhibitors / Q000097:blood*; D006146:Guanidines; D006801:Humans; D007202:Indicators and Reagents; D009439:Neuraminidase / Q000037:antagonists & inhibitors*; D011714:Pyrans; D012015:Reference Standards; D012371:Robotics; D012794:Sialic Acids / Q000097:blood*; D013050:Spectrometry, Fluorescence; D053243:Zanamivir
D004791:Enzyme Inhibitors; D006146:Guanidines; D007202:Indicators and Reagents; D011714:Pyrans; D012794:Sialic Acids; D009439:Neuraminidase; D053243:Zanamivir; D001573:Benzoin