Capacitance measurement to assess acid-induced injury to Salmonella enteritidis PT4.
Capacitance measurement was compared with colony counting procedures for the enumeration and determination of sub-lethal injury of Salmonella enteritidis during storage under varied conditions of pH, acidulant and temperature. Capacitance monitoring was shown to offer an improved technique for the measurement of sub-lethal injury in cell populations. Higher levels of sub-lethal injury were detected by the extension of capacitance detection time than were indicated by differential colony counts on selective and non-selective media. The extension of capacitance detection time noted with sub-lethally injured cell populations was shown to be due to an extended lag phase when cells were placed in the capacitance growth medium and not the result of delayed detection of the growth of a small, uninjured sub-population. Acetic and lactic acids showed both greater lethality and greater ability to inflict sub-lethal injury than the stronger citric or hydrochloric acid. Sub-lethal injury and lethality were not simply related, as little sub-lethal injury was observed with the stronger acids even under conditions that were ultimately highly lethal. The results indicate that weak organic acids cause more reversible damage to cellular sites prior to death: an observation that has implications for choice of resuscitation procedures when examining acidified foods.
School of Biological Sciences, University of Surrey, Guildford, UK.
O
O
Alexandrou
C D
CD
Blackburn
M R
MR
Adams
D000143:Acids / Q000494:pharmacology*; D015169:Colony Count, Microbial / Q000379:methods; D004553:Electric Conductivity; D012477:Salmonella enteritidis / Q000187:drug effects* / Q000254:growth & development*; D013696:Temperature
D000143:Acids