Characterization of murine PACAP mRNA.
A murine PACAP precursor cDNA was isolated by screening a brain cDNA library. The amino acid sequence of the precursor was highly similar (from 81% to 93% similarity) to its rat, human, and ovine counterparts. The primary structure of murine PACAP is identical with those from sheep, humans, and rats, indicating that the mature PACAP is well conserved among mammals. Northern blot analysis revealed that the approximately 2.4 kb transcript for the PACAP precursor is expressed in murine brain. The verification that murine PACAP is identical to its human counterpart provides a rationale for physiological and pathophysiological studies of PACAP in mice.
Discovery Research Laboratories I, Takeda Chemical Industries, Ltd, Ibaraki, Japan.
K
K
Okazaki
Y
Y
Itoh
K
K
Ogi
S
S
Ohkubo
H
H
Onda
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D001923:Brain Chemistry; D017124:Conserved Sequence; D018076:DNA, Complementary / Q000235:genetics; D015723:Gene Library; D051379:Mice; D008813:Mice, Inbred ICR; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000235:genetics*; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; D012333:RNA, Messenger / Q000235:genetics; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
C495402:Adcyap1 protein, mouse; D018076:DNA, Complementary; D009479:Neuropeptides; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; D012333:RNA, Messenger