[Protein composition of venoms from several species of tropical ants and their effect on mitochondrial H+-ATPase].
Protein compositions of venoms of South-American stinging ants, Ectatomma tuberculatum, Paraponera clavata (subfamily Ponerinae), and "tangarana" were analyzed. The venom of E. tuberculatum displayed the most complex protein composition (more than 15 polypeptides). The water-soluble fraction of the venoms of P. clavata and "tangarana" contained acidic proteins (pI < 3.5 to 5.2), whereas the venom of E. tuberculatum contained predominantly basic proteins (pI 8 to > 9.5). N-Terminal residues and N-terminal sequences of a number of polypeptides were determined. High-molecular-mass polypeptides of the P. clavata venom slightly stimulated the ATPase activity of mitochondrial F1-ATPase. Low-molecular-mass nonprotein components of this venom significantly inhibited the ATPase activity of submitochondrial particles and F1-ATPase from bovine heart mitochondria. The venoms of E. tuberculatum and "tangarana" produced no effect on the ATPase activity.
Zaĭtseva
L G
LG
Zaĭtsev
V G
VG
Feniuk
B A
BA
Pavlov
P F
PF
Vilenskaia
N D
ND
Ovchinnikova
T V
TV
Pluzhnikov
K A
KA
Grishin
E V
EV
Grinkevich
V A
VA
D000595:Amino Acid Sequence; D000818:Animals; D000862:Ant Venoms / Q000737:chemistry*; D002417:Cattle; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D007525:Isoelectric Focusing; D007928:Lethal Dose 50; D008929:Mitochondria, Heart / Q000187:drug effects* / Q000201:enzymology; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification / Q000633:toxicity*; D006180:Proton-Translocating ATPases / Q000037:antagonists & inhibitors*; D013045:Species Specificity; D014329:Tropical Climate
D000862:Ant Venoms; D010455:Peptides; D006180:Proton-Translocating ATPases
Issledovanie belkovogo sostava iadov nekotorykh vidov tropicheskikh murav'ev i ikh vliianiia na H+-ATPazu mitokhondriĭ.