Sequencing and identification of expressed Schistosoma mansoni genes by random selection of cDNA clones from a directional library.
We have initiated a gene discovery program in Schistosoma mansoni based on the technique of Expressed Sequence Tags (ESTs), i.e. partial sequences of cDNAs obtained from single passes in automatic DNA sequences. ESTs can be used to identify genes on the basis of their homology with sequences from other species deposited in DNA or protein databases. Transcripts with sequences without matches in the databases may represent novel parasite-specific genes. This approach has shown to be very efficient and in less than two years a broad range of novel genes has already been ascertained, more than doubling the number of known S. mansoni genes.
Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas-UFMG, Belo Horizonte, Brasil.
G R
GR
Franco
A J
AJ
Simpson
S D
SD
Pena
D000818:Animals; D018509:DNA, Helminth / Q000235:genetics*; D015870:Gene Expression; D015723:Gene Library; D012550:Schistosoma mansoni / Q000235:genetics*; D017422:Sequence Analysis, DNA
D018509:DNA, Helminth