Identification of Streptomyces olivaceus Tü 2353 genes involved in the production of the polyketide elloramycin.
The genes for the production of elloramycin (ELM) from Streptomyces olivaceus (So) Tü2353 were cloned using a polyketide synthase gene probe from the tetracenomycin pathway. A cosmid clone (16F4) isolated from a gene library of So Tü2353 conferred tetracenomycin C and ELM resistance to S. lividans TK64 and complemented a mutation in So Tü2353R. Introduction of cosmid 16F4 into S. lividans TK64 resulted in the production of 8-demethyl-tetracenomycin C, an intermediate of ELM biosynthesis.
Decker
H
H
Biologisches Institut, Universität Tübingen, Germany.
Rohr
J
J
Motamedi
H
H
Zähner
H
H
Hutchinson
C R
CR
D000880:Anthraquinones; D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D003001:Cloning, Molecular; D003360:Cosmids; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D009097:Multienzyme Complexes / Q000235:genetics*; D015183:Restriction Mapping; D013302:Streptomyces / Q000235:genetics*
D000880:Anthraquinones; D000900:Anti-Bacterial Agents; D009097:Multienzyme Complexes; C047329:elloramycin