Genes encoding the beta 1 subunit of voltage-dependent Na+ channel in rat, mouse and human contain conserved introns.
We provide evidence in this study that the 86-bp insert in the beta 1.2 mRNA isoform of the voltage gated sodium channel is an intron. Transcripts still retaining this intron were detected in all tissues where the beta 1 gene expression was investigated. We also show that the exon/intron boundaries of the last two introns are conserved among rat, mouse and human beta 1 gene. Unlike the highly conserved cDNAs, introns in only the rat and mouse genes are highly related. The last intron is very short (86-90 bp) and is located in the 3' untranslated sequence, both uncommon properties of mammalian pre-mRNA introns.
Department of Neurology, Yale University School of Medicine, New Haven, CT 06510, USA.
S D
SD
Dib-Hajj
S G
SG
Waxman
D000818:Animals; D001483:Base Sequence; D017124:Conserved Sequence; D006801:Humans; D007438:Introns / Q000235:genetics*; D015640:Ion Channel Gating; D051379:Mice; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D016133:Polymerase Chain Reaction; D012326:RNA Splicing; D051381:Rats; D012689:Sequence Homology, Nucleic Acid; D015222:Sodium Channels / Q000235:genetics*
D015222:Sodium Channels