Studies on the structure and function of the carp gonadotropin alpha subunit by site-directed mutagenesis.
There are two genes encoding the alpha subunit of carp gonadotropin (alpha 1 and alpha 2 subunits). Our previous data have demonstrated that both alpha 1 and alpha 2 subunits expressed in insect cells are able to associate with the beta subunit, but only the alpha 1/beta heterodimer displays biological activity. In the mature protein, there are only four amino-acid residues different between the alpha 1 and alpha 2 subunits. In this study we used site-directed mutagenesis and expressed different mutant alpha subunits in insect cells to identify which residue might be important for biological activity of the alpha subunit. Our results suggested that the change of Arg-71 to Gln-71 affected the activity of the carp gonadotropin alpha subunit.
Huang
C J
CJ
Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan.
Huang
F L
FL
Chang
G D
GD
Chang
Y S
YS
Lo
T B
TB
D000595:Amino Acid Sequence; D000818:Animals; D016367:Baculoviridae / Q000235:genetics; D001483:Base Sequence; D002347:Carps; D015870:Gene Expression; D015292:Glycoprotein Hormones, alpha Subunit / Q000737:chemistry* / Q000235:genetics / Q000494:pharmacology; D008297:Male; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D011994:Recombinant Proteins; D018411:Spodoptera / Q000378:metabolism; D013329:Structure-Activity Relationship; D013737:Testis / Q000187:drug effects; D014162:Transfection
D015292:Glycoprotein Hormones, alpha Subunit; D011994:Recombinant Proteins