Separation of sister chromatids in mitosis requires the Drosophila pimples product, a protein degraded after the metaphase/anaphase transition.
Mutations in the Drosophila genes pimples and three rows result in a defect of sister chromatid separation during mitosis. As a consequence, cytokinesis is also defective. However, cell cycle progression including the mitotic degradation of cyclins A and B is not blocked by the failure of sister chromatid separation, and as a result, metaphase chromosomes with twice the normal number of chromosome arms still connected in the centromeric region are observed in the following mitosis, pimples encodes a novel protein that is rapidly degraded in mitosis. Our observations suggest that Pimples and Three rows act during mitosis to release the cohesion between sister centromeres.
Friedrich-Miescher-Laboratorium der Max-Planck-Gesellschaft, Tübingen, Federal Republic of Germany.
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D000595:Amino Acid Sequence; D000705:Anaphase / Q000235:genetics*; D000818:Animals; D000953:Antigens, Protozoan; D001483:Base Sequence; D015152:Blotting, Northern; D018797:Cell Cycle Proteins / Q000235:genetics* / Q000378:metabolism; D002455:Cell Division / Q000235:genetics; D002503:Centromere / Q000235:genetics / Q000378:metabolism; D002842:Chromatids / Q000235:genetics*; D002875:Chromosomes / Q000235:genetics / Q000378:metabolism; D003001:Cloning, Molecular; D016213:Cyclins / Q000378:metabolism; D004330:Drosophila / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental / Q000235:genetics; D017344:Genes, Insect / Q000502:physiology; D017404:In Situ Hybridization, Fluorescence; D007814:Larva / Q000235:genetics; D008677:Metaphase / Q000235:genetics*; D008938:Mitosis / Q000235:genetics; D008969:Molecular Sequence Data; D009154:Mutation / Q000502:physiology; D010641:Phenotype; D015800:Protozoan Proteins / Q000235:genetics
D000953:Antigens, Protozoan; D018797:Cell Cycle Proteins; D016213:Cyclins; D015800:Protozoan Proteins; C095023:antigen PIM, Theileria parva