Localization and possible gene expression of proteoglycan decorin in the trabecular meshwork.
It is known that trabecular meshwork cells produce proteoglycans and that local production may be associated with aqueous outflow resistance. In an attempt to identify intraocular production of proteoglycan decorin in the anterior chamber angle of mammalian eyes, we conducted a Northern blot analysis and immunohistochemical studies. Northern blot analysis suggested gene expression of proteoglycan decorin in trabecular meshwork cells. Also, immunohistochemical studies using anti-decorin antibody demonstrated decorin-like immunoreactivity in the trabecular meshwork and around the Schlemm's canal. Our data demonstrate the presence of proteoglycan decorin in the outflow pathway, suggesting that decorin is a component of extracellular matrices in these regions and may be associated with outflow resistance.
Tanihara
H
H
Doheny Eye Institute, Los Angeles, CA 90033, USA.
Ohira
A
A
Takahashi
M
M
Honda
Y
Y
Suzuki
S
S
D000818:Animals; D000867:Anterior Chamber / Q000378:metabolism*; D015152:Blotting, Northern; D002417:Cattle; D002478:Cells, Cultured; D058575:Decorin; D004587:Electrophoresis, Agar Gel; D016326:Extracellular Matrix Proteins; D015870:Gene Expression; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D008875:Middle Aged; D011509:Proteoglycans / Q000096:biosynthesis* / Q000235:genetics; D012313:RNA / Q000032:analysis; D014129:Trabecular Meshwork / Q000166:cytology / Q000378:metabolism*
C546878:DCN protein, human; D058575:Decorin; D016326:Extracellular Matrix Proteins; D011509:Proteoglycans; D012313:RNA