Vitamin E protects intestinal basolateral membrane from CMF-induced damages in rat.
CMF is a combination of anticancer chemotherapeutic agents Cyclophosphamide, Methotrexate and 5-Fluorouracil. Vitamin E protects the basolateral membrane (BSM) from CMF induced lipid peroxidative damages. Rats were treated intravenously with cyclophosphamide-10 mg, methotrexate-1.0 mg and 5-fluorouracil-10 mg per kg body weight for six cycles. Vitamin E (600 mg/kg body weight) was administered orally, daily. Intestinal basolateral membrane bound ATPases (3.6.1.3), Alkalinephosphatase (3.1.1) and 5'-Nucleotidase (3.1.3.5) were protected by co-administration of vitamin E with CMF. In CMF treated rats the lipid peroxidation levels were found to be elevated with a significant depletion in membrane sulfhydryl groups. In vitamin E co-administered animals, the enzyme activities were found to be restored with concomitant reduction in malondialdehyde levels and an increase in the sulfhydryl groups. The membrane cholesterol and phospholipid levels which were altered in CMF treated rats were bought back to the normal in co-administration of vitamin E.
Department of Biochemistry, University of Madras.
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Subramaniam
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Subramaniam
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Shyamala Devi
D000818:Animals; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D003520:Cyclophosphamide / Q000037:antagonists & inhibitors / Q000633:toxicity; D005472:Fluorouracil / Q000037:antagonists & inhibitors / Q000633:toxicity; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*; D050356:Lipid Metabolism; D008054:Lipid Peroxides / Q000378:metabolism; D008297:Male; D008566:Membranes / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D008727:Methotrexate / Q000037:antagonists & inhibitors / Q000633:toxicity; D051381:Rats; D017208:Rats, Wistar; D013972:Thyrotropin / Q000378:metabolism; D014810:Vitamin E / Q000494:pharmacology*
D008054:Lipid Peroxides; D014810:Vitamin E; D003520:Cyclophosphamide; D013972:Thyrotropin; D005472:Fluorouracil; D008727:Methotrexate