Liver microsomal phospholipid fatty acids behavior and its relationship to bilirubin UDP-glucuronyltransferase activity in bile duct ligated rats.
Phospholipid fatty acid composition and bilirubin UDP-glucuronyltransferase activity from liver microsomal membrane were studied in normal and in bile duct ligated rats. Incubation of normal microsomes with 15 microM bilirubin (considered as physiological concentration) yielded 60% bilirubin diglucuronide; in 2 days post-cholestatic rats, they showed 20% bilirubin diglucuronide which was undetectable in 8 days post-cholestatic group. When compared to controls, after 2 days of cholestasis, microsomal phospholipids showed a clear decrease in linoleic and arachidonic acids and an increment in palmitic and stearic acids. 8 days post-cholestatic rats presented a marked increase in palmitic, oleic and docosaexaenoic acids, while linoleic and arachidonic acids decreased. Cholestasis produced disturbances in microsomal phospholipids fatty acid composition; but these changes are unable to explain entirely the severe damage observed in bilirubin diglucuronide formation.
Ouviña
C B
CB
Cátedra de Fisiopatología, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Argentina.
Pavese
C B
CB
Lemberg
A
A
Bengochea
L A
LA
D000818:Animals; D002779:Cholestasis / Q000201:enzymology / Q000378:metabolism*; D003250:Constriction; D005227:Fatty Acids / Q000032:analysis*; D014453:Glucuronosyltransferase / Q000378:metabolism*; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism*; D010743:Phospholipids / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar
D005227:Fatty Acids; D010743:Phospholipids; D014453:Glucuronosyltransferase