Characteristics of equine herpesvirus 1 glycoproteins expressed in insect cells.
A series of recombinant baculoviruses containing genes for glycoproteins C, D, H and L of equine herpesvirus 1 (EHV-1) have been constructed, and the EHV-1 products characterised by gel electrophoresis and immunoblotting. The EHV-1 glycoproteins expressed in insect cells were similar but not identical in apparent sizes to those expressed in EHV-1 infected mammalian cells. Each of the EHV-1 products was recognised by convalescent equine sera, indicating that they were all targets for an equine immune response. Mice immunised with baculovirus-expressed EHV-1 gD and gC acquired an enhanced ability to clear challenge EHV-1 from respiratory tissues, in association with both neutralising antibody and cell mediated immune responses.
School of Biological Sciences, Macquarie University, Sydney, NSW, Australia.
J M
JM
Whalley
D N
DN
Love
D
D
Tewari
H J
HJ
Field
D000818:Animals; D016367:Baculoviridae; D015153:Blotting, Western; D002460:Cell Line; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005455:Fluorescent Antibody Technique; D006566:Herpesviridae Infections / Q000276:immunology / Q000662:veterinary*; D004861:Herpesvirus 1, Equid / Q000235:genetics* / Q000378:metabolism; D006734:Horse Diseases; D006736:Horses; D008322:Mammals; D051379:Mice; D008807:Mice, Inbred BALB C; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000276:immunology; D018411:Spodoptera; D014162:Transfection; D014759:Viral Envelope Proteins / Q000096:biosynthesis* / Q000276:immunology* / Q000302:isolation & purification; D014765:Viral Vaccines / Q000096:biosynthesis
D011994:Recombinant Proteins; D014759:Viral Envelope Proteins; D014765:Viral Vaccines