Effect of curcumin on certain lysosomal hydrolases in isoproterenol-induced myocardial infarction in rats.
The effect of curcumin on lysosomal hydrolases in serum and heart was studied by determining the activities of beta-glucuronidase, beta-N-acetylglucosaminidase, cathepsin B, cathepsin D, and acid phosphatase. Rats treated with isoproterenol (30 mg/100 g body weight) showed a significant increase in serum lysosomal hydrolase activities, which were found to decrease after curcumin treatment. Isoproterenol administration to rats resulted in decreased stability of the membranes, which was reflected by the lowered activity of cathepsin D in mitochondrial, lysosomal, and microsomal fractions. Curcumin treatment returned the activity levels almost to normal, showing that curcumin restored the normal function of the membrane. Histopathological studies of the infarcted rat heart also showed a decreased degree of necrosis after curcumin treatment.
Nirmala
C
C
Department of Biotechnology, Central Leather Research Institute, Madras, India.
Puvanakrishnan
R
R
D000818:Animals; D002402:Cathepsin D / Q000378:metabolism*; D003474:Curcumin / Q000494:pharmacology*; D005260:Female; D007425:Intracellular Membranes / Q000187:drug effects; D007545:Isoproterenol; D008247:Lysosomes / Q000187:drug effects / Q000201:enzymology*; D009203:Myocardial Infarction / Q000139:chemically induced / Q000201:enzymology*; D009206:Myocardium / Q000201:enzymology; D051381:Rats; D017208:Rats, Wistar
D002402:Cathepsin D; D003474:Curcumin; D007545:Isoproterenol