Common clonal chromosome aberrations in cytokine-dependent continuous human T-lymphocyte cell lines.
Antigen-mediated T-cell proliferation is a transient phenomenon. Like other somatic cells, T lymphocytes generally show replicative senescence in vitro. However, we here show that cytokine-dependent continuous (immortal) T-cell lines can be established from skin biopsy specimens of inflammatory skin diseases. Continuous growth can be obtained by culturing T cells in medium supplemented with interleukin-2 and interleukin-4, but without antigen or antigen-presenting cells added. Loss of the T-cell antigen receptor complex is observed in some of the continuous T-cell lines. Most T-cell lines develop clonal chromosome aberrations during continuous growth. Aberrations for chromosomes 1, 2, 8, 16, and 18 are most commonly observed.
Department of Human Genetics, Marselisborg University Hospital, Aarhus, Denmark.
K
K
Kaltoft
B H
BH
Hansen
C B
CB
Pedersen
S
S
Pedersen
K
K
Thestrup-Pedersen
D002455:Cell Division; D002460:Cell Line; D002869:Chromosome Aberrations; D016207:Cytokines / Q000494:pharmacology*; D003872:Dermatitis / Q000473:pathology; D006801:Humans; D016130:Immunophenotyping; D007376:Interleukin-2 / Q000494:pharmacology; D015847:Interleukin-4 / Q000494:pharmacology; D016410:Lymphoma, T-Cell, Cutaneous / Q000235:genetics* / Q000473:pathology; D012867:Skin; D012878:Skin Neoplasms / Q000235:genetics* / Q000473:pathology; D013601:T-Lymphocytes / Q000276:immunology / Q000648:ultrastructure*; D014407:Tumor Cells, Cultured
D016207:Cytokines; D007376:Interleukin-2; D015847:Interleukin-4