Antisense oligodeoxynucleotides against mu- or kappa-opioid receptors block agonist-induced body temperature changes in rats.
PL017 and dynorphin A1-17 were shown previously to cause a marked increase and a profound decrease in body temperature (Tb), respectively. In this study, we examined whether an antisense (AS) oligodeoxynucleotide (oligo) against cloned mu or kappa opioid receptors could block PL017- or dynorphin A-induced body temperature changes. Treatment with an AS oligo against mu receptors, but not sense (S) oligo, missense (MS) oligo or artificial cerebrospinal fluid (aCSF), abolished PL017-induced hyperthermia. In addition, treatment with an AS oligo against kappa receptors, but not S oligo, MS oligo or aCSF, greatly attenuated dynorphin A-induced hypothermia. This study further supports the notion that mu and kappa receptors mediate Tb regulation.
Chen
X H
XH
Department of Pharmacology, Temple University School of Medicine, Philadelphia, PA 19140, USA.
Geller
E B
EB
de Riel
J K
JK
Liu-Chen
L Y
LY
Adler
M W
MW
D000818:Animals; D001483:Base Sequence; D001833:Body Temperature Regulation / Q000187:drug effects*; D004399:Dynorphins / Q000037:antagonists & inhibitors; D004723:Endorphins / Q000037:antagonists & inhibitors; D007276:Injections, Intraventricular; D015394:Molecular Structure; D016376:Oligonucleotides, Antisense / Q000494:pharmacology*; D051381:Rats; D017473:Receptors, Opioid, kappa / Q000187:drug effects*; D017450:Receptors, Opioid, mu / Q000187:drug effects*
D004723:Endorphins; D016376:Oligonucleotides, Antisense; D017473:Receptors, Opioid, kappa; D017450:Receptors, Opioid, mu; D004399:Dynorphins; C044708:morphiceptin, N-Me-Phe(3)-