Glutathione-S-transferase (GST) theta polymorphism influences background SCE rate.
Polymorphism of glutathione S-transferase theta (GSTT1) modulates the toxicity of halogenated alkanes and epoxides in humans. The enzymatic activity of glutathione S-transferase theta and its corresponding gene is lacking in about 30% of the central European population. It has now been demonstrated that the background rate for sister chromatid exchange (SCE) is affected by this particular polymorphism. Smoking as a known inducer of SCE was taken into account. A group of GSTT1-positive subjects exhibited lower SCE rates than GSTT1-negative individuals (7.55 +/- 0.77 versus 8.74 +/- 1.24 SCE/mitosis, respectively, p < 0.005). Non-smoking GSTT1-positive individuals showed the lowest SCE rate (7.26 +/- 0.71 SCE/mitosis), significantly lower than the rates of smoking GSTT1-positive and non-smoking GSTT1-negative subjects (8.14 +/- 0.55 SCE/mitosis and 8.12 +/- 0.88 SCE/mitosis, respectively, p < 0.025 in both cases). Smoking GSTT1-negative subjects exhibited the highest SCE rates (9.28 +/- 1.3 SCE/mitosis). It is hypothesized that GSTT1 is protective against background genotoxic damage. Since ethylene oxide is a proven substrate of GSTT1, the detoxification of this epoxide arising from endogenous ethylene may modulate SCE background rates.
Schröder
K R
KR
Institut für Arbeitsphysiologie, Universität Dortmund, Germany.
Wiebel
F A
FA
Reich
S
S
Dannappel
D
D
Bolt
H M
HM
Hallier
E
E
D000328:Adult; D016009:Chi-Square Distribution; D004912:Erythrocytes / Q000201:enzymology; D005027:Ethylene Oxide / Q000378:metabolism; D005260:Female; D005982:Glutathione Transferase / Q000097:blood / Q000235:genetics*; D006801:Humans; D008297:Male; D008938:Mitosis / Q000235:genetics; D011110:Polymorphism, Genetic; D012854:Sister Chromatid Exchange / Q000235:genetics*; D012907:Smoking / Q000009:adverse effects; D013379:Substrate Specificity
D005982:Glutathione Transferase; D005027:Ethylene Oxide