Relationship between clinical manifestations of footrot and specific DNA products of Dichelobacter nodosus amplified through PCR.
A total of 141 Dichelobacter nodosus isolates from 46 merino sheep farms with various clinical forms of footrot was examined by the gelatin gel test and the polymerase chain reaction (PCR) using virulent (Vf2 and Vr2) and benign (Bf and Br) specific primers. Isolates from sheep with virulent and high intermediate footrot usually produced relatively thermostable proteases, but a decreasing proportion of the isolates from sheep with medium and low intermediate or benign footrot had thermostable proteases, as determined by the gelatin gel test. The amplification by PCR of a major band of 857 bp by Vf2 and Vr2 was often associated with isolates from the more virulent forms of footrot whereas the presence of a major band of 1300 bp by Vf2 and Vr2 and/or a band of 609 bp by Bf and Br was associated with isolates from less virulent forms of footrot. Nevertheless, the virulent and benign gene regions represented by Vf2 and Vr2 and Bf and Br are only two of the many factors involved in determining the virulence of D nodosus. As a result the relationship observed between the clinical manifestations of footrot and specific DNA products amplified by PCR was not complete.
Liu
D
D
Department of Agriculture, Pastoral and Veterinary Institute, Hamilton, Victoria, Australia.
Roycroft
C
C
Samuel
J
J
Webber
J
J
D000818:Animals; D001421:Bacteria, Anaerobic / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D001424:Bacterial Infections / Q000175:diagnosis / Q000453:epidemiology / Q000662:veterinary*; D001483:Base Sequence; D017931:DNA Primers; D005121:Extremities; D005535:Foot Rot / Q000175:diagnosis* / Q000453:epidemiology / Q000503:physiopathology; D015994:Incidence; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D012756:Sheep; D012757:Sheep Diseases / Q000453:epidemiology; D014739:Victoria / Q000453:epidemiology; D014774:Virulence
D017931:DNA Primers