A new fluorochromasia method using a fluorescence microplate reader for assay of cytotoxic activity of carp leucocytes.
A new fluorochromasia method using a fluorescence microplate reader has been established to assay spontaneous cytotoxic activity of carp leucocytes. This method is characterized by using propidium iodide (PI) for staining dead target cells and a fluorescence microplate reader for measurement of the fluorescence of PI. K562 as target cells were prepared in 96-well flat-bottom microplates, and carp leucocytes were added as effector cells. After 2.5 h incubation, PI was added to each well. After an additional 1.5 h incubation, fluorescence of each well was measured. Correlation between this method and 51Cr-release assay was obtained. The results demonstrated that this new fluorochromasia method can be used to assay cytotoxic activity of carp leucocytes.
Sano
N
N
Department of Aquatic Biosciences, Tokyo University of Fisheries, Japan.
Kurata
O
O
Okamoto
N
N
Ikeda
Y
Y
D000818:Animals; D002347:Carps / Q000276:immunology*; D003601:Cytotoxicity Tests, Immunologic / Q000379:methods / Q000662:veterinary*; D003602:Cytotoxicity, Immunologic / Q000276:immunology*; D005260:Female; D007962:Leukocytes / Q000276:immunology*; D008297:Male; D011419:Propidium; D013050:Spectrometry, Fluorescence / Q000662:veterinary
D011419:Propidium