Immunohistochemical detection of canine leucocyte antigens by specific monoclonal antibodies in canine normal tissues.
This report describes the immunocytochemical detection and distribution of different canine leucocytes antigens (Thy-1, CD5, CD4, CD8, MHC-II and B-cell antigen) by means of monoclonal antibodies (mAbs) in frozen tissue sections of lymphoid organs of the dog. CD5 is expressed exclusively by T lymphocytes. Thy-1 is expressed by T-lymphocytes and in a variety of non-lymphoid tissues. The mAb against canine MHC-II could detect B and T lymphocytes, macrophages, fibroblasts and antigen presenting cells. The CD4 antigen is expressed on many lymphocytes of the T-dependent areas of lymph organs and is also expressed by certain antigen presenting cells. CD8 is expressed in a minor proportion of T cells. The mAb against B cells detected lymphocytes in lymphoid follicles and epithelial cells of the thymic medulla.
Departament de Patologia i Producció Animals, Facultat de Veterinària, Universitat Autònoma de Barcelona, Bellaterra, Spain.
R M
RM
Rabanal
L
L
Ferrer
R W
RW
Else
D000818:Animals; D000911:Antibodies, Monoclonal; D000938:Antigen-Presenting Cells / Q000276:immunology; D015703:Antigens, CD / Q000032:analysis; D001402:B-Lymphocytes / Q000276:immunology; D004285:Dogs / Q000276:immunology*; D006649:Histocompatibility Antigens / Q000032:analysis*; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D007124:Immunoenzyme Techniques / Q000662:veterinary; D007668:Kidney / Q000276:immunology; D008099:Liver / Q000276:immunology; D008221:Lymphoid Tissue / Q000276:immunology*; D012867:Skin / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology; D018800:Thy-1 Antigens / Q000032:analysis
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D006649:Histocompatibility Antigens; D000949:Histocompatibility Antigens Class II; D018800:Thy-1 Antigens