[Evaluation of gastric adaptive relaxation in isolated stomach from the guinea-pig].
To evaluate the mechanism of gastric adaptive relaxation (GAR), we designed and established the experimental system for the measurement of GAR in isolated stomach from the guinea-pig by modifying the method of Desai. We also investigated the roles of non-adrenergic, non-cholinergic (NANC) nerves and endogenous nitric oxide (NO) in GAR by using this system. The rapid increase in the capacity of isolated stomach was observed over a certain pressure as GAR. GAR was abolished by tetrodotoxin (10(-6) M) in the presence of atropine (3 x 10(-6) M) and guanethidine (5 x 10(-6) M). NG-nitro L-arginine (LNNA) (10(-4) M), a NO synthesis inhibitor, also abolished GAR. L-arginine (10(-3) M), a precursor for NO synthesis, reversed LNNA-induced impairment of GAR. Sodium nitroprusside (10(-6)-10(-4) M), a NO-donor, induced the gastric relaxation. These results suggest that GAR is mediated by NANC nerves, possibly via endogenous NO.
Third Department of Internal Medicine, Osaka City University Medical School.
H
H
Uno
T
T
Arakawa
T
T
Fukuda
K
K
Higuchi
T
T
Hayakawa
Y
Y
Kase
S
S
Takeda
K
K
Kobayashi
D000222:Adaptation, Physiological; D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives / Q000494:pharmacology; D001341:Autonomic Nervous System / Q000502:physiology; D006168:Guinea Pigs; D066298:In Vitro Techniques; D008297:Male; D009126:Muscle Relaxation / Q000187:drug effects; D009569:Nitric Oxide / Q000378:metabolism / Q000502:physiology; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors; D019335:Nitroarginine; D009599:Nitroprusside / Q000494:pharmacology; D013270:Stomach / Q000502:physiology*; D013779:Tetrodotoxin / Q000494:pharmacology
D009599:Nitroprusside; D019335:Nitroarginine; D009569:Nitric Oxide; D013779:Tetrodotoxin; D001120:Arginine; D019001:Nitric Oxide Synthase