Isolation of candidate genes by subtractive and sequential (Boolean) hybridization: an hypothesis.
This paper describes a theoretical method of isolating candidate disease-specific genes by polymerase chain reaction (PCR)-based subtractive hybridization and subsequent (Boolean) hybridization of the subtracted library to arrays of chromosome-specific cosmid clones. A specific application of this technique to isolate genes potentially related to Wilson's disease is outlined.
Liver Unit, National Institute for Health, Bethesda, Maryland 20892, USA.
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R
Sallie
D001483:Base Sequence; D002874:Chromosome Mapping; D002882:Chromosomes, Human, Pair 13; D004247:DNA / Q000235:genetics / Q000302:isolation & purification*; D017931:DNA Primers; D015723:Gene Library; D005796:Genes; D030342:Genetic Diseases, Inborn / Q000235:genetics*; D006527:Hepatolenticular Degeneration / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction / Q000379:methods*
D017931:DNA Primers; D004247:DNA