Quantification of DNA damage and repair in amino acid auxotrophs and UV-sensitive mutants of Aspergillus nidulans using an ELISA.
An ELISA used to investigate DNA repair in mammalian cells has been adapted to investigate mutagen-induced DNA damage and repair in protoplasts of Aspergillus nidulans. The assay shows a reduced rate of repair of DNA damage in methionine and arginine auxotrophs (methG and argB), which were shown previously to be hypersensitive to UV radiation and chemical mutagens. The assay also showed a considerably reduced ability to repair mutagen-induced damage in the uv-sensitive mutants uvsB and uvsH. The increased sensitivity of amino acids auxotrophs to mutagens is, therefore, correlated with a reduced capacity to repair mutagen-induced DNA damage.
School of Applied Biological and Chemical Sciences, University of Ulster at Jordanstown, Northern Ireland, UK.
E
E
Donnelly
Y A
YA
Barnett
W
W
McCullough
D015112:4-Nitroquinoline-1-oxide / Q000494:pharmacology; D001120:Arginine / Q000378:metabolism*; D001233:Aspergillus nidulans / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification / Q000528:radiation effects; D004249:DNA Damage; D004260:DNA Repair; D004271:DNA, Fungal / Q000187:drug effects / Q000235:genetics / Q000528:radiation effects; D004277:DNA, Single-Stranded / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D008715:Methionine / Q000378:metabolism*; D008741:Methyl Methanesulfonate / Q000494:pharmacology; D016296:Mutagenesis; D009153:Mutagens / Q000494:pharmacology; D011523:Protoplasts; D014466:Ultraviolet Rays
D004271:DNA, Fungal; D004277:DNA, Single-Stranded; D009153:Mutagens; D015112:4-Nitroquinoline-1-oxide; D001120:Arginine; D008715:Methionine; D008741:Methyl Methanesulfonate