[Comparison of platelet and leukocyte content in platelet concentrates obtained from CS-3000 and third generation (CS-3000 plus and Cobe-Spectra) cell separators].
Platelet concentrates (PCs) obtained using old generation of cell separators contain high number of leukocytes. White cell (WBC) contamination and platelet (Plts) number in PCs obtained from separator II-nd generation CS-3000 and separators III-rd generations CS-3000 plus and Cobe-Spectra have been determined. PCs from new separators contain the same Plts number as PCs from CS-3000. The average leukocyte count in PCs obtained from CS-3000 was 171.26 x 10(6), whereas WBC number in PCs from CS-3000 plus and Cobe-Spectra were 2.87 and 2.54 x 10(6) respectively. In about 85% of PCs obtained from III-rd generation cell separators the leukocyte count did not exceed 5 x 10(6). This count is considered sufficient to prevent alloimmunization of HLA antigens. The determination of WBC count in every PCs allows to select PCs with fewer than 5 x 10(6) leukocytes and to transfuse them without the necessity of using expensive filters for leukocyte removing.
Zakładu Transfuzjologii i Organizacji Słuzby Krwi, Instytutu Hematologii i Transfuzjologii w Warszawie.
A
A
Dzieciatkowska
M
M
Fuksiewicz
E
E
Lachert
B
B
Ziemba-Zółtowska
M
M
Letowska
D001792:Blood Platelets / Q000276:immunology; D002469:Cell Separation / Q000295:instrumentation* / Q000592:standards*; D004866:Equipment Contamination; D004867:Equipment Design; D005374:Filtration; D006680:HLA Antigens / Q000276:immunology; D006801:Humans; D007126:Immunoglobulin Allotypes / Q000276:immunology; D007958:Leukocyte Count; D010976:Platelet Count
D006680:HLA Antigens; D007126:Immunoglobulin Allotypes
Porównanie zawartości płytek krwi i leukocytów w koncentratach krwinek płytkowych, otrzymanych przy uzyciu separatora CS-3000 oraz separatorów III generacji (CS-3000 plus i Cobe-Spectra).