A self-defense gene homologous to tetracycline effluxing gene essential for antibiotic production in Streptomyces aureofaciens.
By Northern blot analyses with DNA probes carrying 6-demethylchlortetracycline (6-DCT) biosynthetic genes from Streptomyces aureofaciens NRRL3203, a highly expressed gene (tcrC) was detected in a high titer producing mutant derived from the parental strain NRRL3203 by NTG mutagenesis. The analysis of the nucleotide sequence of the 2.8-kb BamHI fragment containing tcrC gene showed that the predicted tcrC gene product is a protein consisting of 512 amino acids. The deduced amino acid sequence had a high level identity with that of the self-defense gene (tet347) of Streptomyces rimosus, known to mediate oxytetracycline efflux. The tcrC gene-inactivated strains generated from strain NRRL3203 by gene replacement had a 90% decrease in the level of resistance to tetracycline and the antibiotic productivity when compared with the parental strain.
Tokyo Research Laboratories of Kyowa Hakko Kogyo Co., Ltd., Japan.
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Dairi
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Aisaka
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Katsumata
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Hasegawa
D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000378:metabolism / Q000494:pharmacology; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial; D015870:Gene Expression; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D013304:Streptomyces aureofaciens / Q000235:genetics* / Q000378:metabolism; D013752:Tetracycline / Q000378:metabolism / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D013752:Tetracycline