Morphologic study of the laryngeal taste buds in the cat.
The distribution of laryngeal taste buds (TBs) and their neutral components in the cat were investigated by immunohistochemistry and electron microscopy. The antisera used in this study were against cytokeratin, protein gene product 9.5 (PGP9.5), neuron-specific enolase (NSE), S-100 protein, calbindin D, calcitonin gene-related peptide (CGRP), and substance P (SP). Taste bud cells were specifically immunoreactive to the antibodies of human cytokeratin subtypes 8 and 18 (CAM5.2). On observation with CAM5.2, TBs were seen distributed on the laryngeal surface of the epiglottis and spread caudally along the aryepiglottic folds, reaching peak density at the laryngeal side of the arytenoid tubercle. The PGP9.5 and NSE immunoreactivities were recognized in TB cells and nerve fibers, both within the TBs and in the subepithelial connective tissue. S-100 protein immunoreactivities were not found in any of the cells in the TBs but were found exclusively in the subepithelial neural elements. The calbindin-D, CGRP, and SP immunoreactivities were confined to a part of the neural elements that was very thin. Taste pores, taste villi, neuronal varicosity, and synapselike structures were observed by scanning and transmission electron microscopic study. From these results it is considered that the TBs act as a chemical receptor.
Department of Otolaryngology, Saga Medical School, Japan.
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Shin
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Nahm
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Maeyama
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Miyazaki
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Matsuo
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Yu
D000818:Animals; D000906:Antibodies; D001193:Arytenoid Cartilage / Q000033:anatomy & histology / Q000294:innervation; D064026:Calbindins; D015740:Calcitonin Gene-Related Peptide / Q000032:analysis; D002415:Cats; D003238:Connective Tissue / Q000033:anatomy & histology / Q000294:innervation; D004825:Epiglottis / Q000033:anatomy & histology / Q000294:innervation; D004848:Epithelium / Q000033:anatomy & histology / Q000294:innervation; D006801:Humans; D007150:Immunohistochemistry; D007633:Keratins / Q000032:analysis; D007830:Larynx / Q000033:anatomy & histology*; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D009412:Nerve Fibers / Q000648:ultrastructure; D009419:Nerve Tissue Proteins / Q000032:analysis; D009474:Neurons / Q000648:ultrastructure; D010751:Phosphopyruvate Hydratase / Q000032:analysis; D064030:S100 Calcium Binding Protein G / Q000032:analysis; D009418:S100 Proteins / Q000032:analysis; D013373:Substance P / Q000032:analysis; D013650:Taste Buds / Q000033:anatomy & histology*; D013869:Thiolester Hydrolases / Q000032:analysis; D043222:Ubiquitin Thiolesterase
D000906:Antibodies; D064026:Calbindins; D009419:Nerve Tissue Proteins; D064030:S100 Calcium Binding Protein G; D009418:S100 Proteins; D013373:Substance P; D007633:Keratins; D013869:Thiolester Hydrolases; D043222:Ubiquitin Thiolesterase; D010751:Phosphopyruvate Hydratase; D015740:Calcitonin Gene-Related Peptide