Isolation of serotype 1 Marek's disease viruses from vaccinated Australian flocks.
Field samples were received for Marek's disease virus (MDV) isolation from clinically affected flocks from several regions of Eastern Australia. Lymphocytes were fractionated in Ficoll-Paque and passaged once or twice in chicken embryo kidney cultures. Serotype-specific virus was detected in infected cultures by indirect immunofluorescence with monoclonal antibodies. Serotype 1 MDV was isolated from 10 flocks. In samples from 5 of these flocks, serotype 2 and 3 vaccine viruses were isolated from the same specimen. In a parallel study, plasmas obtained during lymphocyte isolation were tested for antibodies to MDVs by agar gel precipitin (AGP) tests using serotype 1 and 3 antigen extracts. No correlation was observed between the rate of virus isolation and AGP positivity. The AGP test was incapable of discriminating between the different MDV serotypes.
Department of Applied Biology and Biotechnology Royal Melbourne Institute of Technology Melbourne, Victoria, Australia.
D B
DB
De Laney
A E
AE
Jones
M
M
Zerbes
G A
GA
Tannock
D000818:Animals; D001315:Australia; D002478:Cells, Cultured; D002642:Chick Embryo; D002645:Chickens; D019084:Fluorescent Antibody Technique, Indirect; D008381:Herpesvirus 2, Gallid / Q000145:classification* / Q000302:isolation & purification; D007668:Kidney; D008214:Lymphocytes / Q000276:immunology* / Q000821:virology*; D008380:Marek Disease / Q000276:immunology* / Q000517:prevention & control; D011200:Poultry; D011233:Precipitin Tests; D012703:Serotyping; D014611:Vaccination; D014765:Viral Vaccines
D014765:Viral Vaccines