A strategy for the detection of de novo synthesis of nucleic acids: an hypothesis.
This paper outlines novel strategy for demonstrating de novo synthesis of nucleic acids applicable, at least, to the detection of viral replication within cell culture or other in vitro systems. The method uses an incorporation capture technique which is suitable for the detection of new and on-going synthesis of both RNA and DNA of any specific sequence. When combined with random RNA (or DNA) amplification by polymerase chain reaction (PCR), and subsequent application of well established cloning techniques, this strategy will allow the construction cDNA libraries of newly synthesized RNAs.
Sallie
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Liver Diseases Section, NIDDK, National Institutes of Health, Bethesda, Maryland 20892, USA.
D000818:Animals; D001483:Base Sequence; D002467:Cell Nucleus / Q000378:metabolism; D002478:Cells, Cultured; D003001:Cloning, Molecular / Q000379:methods; D004247:DNA / Q000032:analysis / Q000096:biosynthesis*; D015723:Gene Library; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012313:RNA / Q000032:analysis / Q000096:biosynthesis*
D012313:RNA; D004247:DNA