High molecular weight polypeptide bands specific for equine herpesvirus 4.
Serum neutralisation (SN) and immunoblotting were used in attempts to distinguish between natural infections with the closely related viruses equine herpesvirus 1 (EHV-1) and equine herpes-virus 4 (EHV-4). Horse sera (n = 323) collected in 1990 from studs with no experience of EHV-1 abortions as well as 197 sera collected in 1992 from studs with a history of EHV-1 abortions were tested by SN. The two groups differed in the proportion with measurable EHV-1 antibody, the 1992 group being significantly higher. Both groups had high proportions with EHV-4 antibody and no serum had antibody to EHV-1 alone. Pools of positive sera were prepared as probes in immunoblots. High molecular weight (> 200 kDa) polypeptide bands specific for EHV-4 were detected. No bands specific for EHV-1 only were found. The specific EHV-4 polypeptides shared some properties with gp2 of EHV-1.
Stanford Consulting Laboratory, Rydalmere, NSW, Australia.
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D000034:Abortion, Veterinary; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood* / Q000276:immunology; D000918:Antibody Specificity; D000956:Antigens, Viral / Q000276:immunology / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006566:Herpesviridae Infections / Q000276:immunology / Q000662:veterinary*; D004861:Herpesvirus 1, Equid / Q000276:immunology*; D006734:Horse Diseases; D006736:Horses; D015151:Immunoblotting; D008297:Male; D008970:Molecular Weight; D009500:Neutralization Tests; D011247:Pregnancy; D013045:Species Specificity; D018140:Varicellovirus / Q000276:immunology*; D014764:Viral Proteins / Q000276:immunology* / Q000302:isolation & purification*
D000914:Antibodies, Viral; D000956:Antigens, Viral; D014764:Viral Proteins