Diversity of immunobiological functions of T-cell lines established from patients with adult T-cell leukaemia.
In order to understand the variety of HTLV-1-associated cutaneous diseases, we studied the cytological profile of HTLV-1-infected T-cell lines established from patients with adult T-cell leukaemia (ATL). Among four CD4+ cell lines, termed 16T(-), 35T(-), MH-1, and KS-2, the 16T(-) cells secreted elevated quantities of IL-4, IL-6 and IFN-gamma and expressed mRNA for each cytokine in the absence of exogenous stimulation. The 35T(-) cells secreted IL-6 and a small amount of IFN-gamma, but not IL-4. The MH-1 and KS-2 cells secreted only IL-6 in the absence of stimulation. In response to stimulation with phorbol-12-myristate-13 acetate (PMA), the 16T(-) cells produced more IL-4 and IFN-gamma, whereas the 35T(-) and MH-1 cells exhibited increased secretion of IFN-gamma, but still no IL-4 or IL-4 mRNA production. Although neither IL-4 nor IFN-gamma were found in the culture supernatant of KS-2 cells, the production of IL-4 mRNA was detected by RT-PCR. Culture supernatants from the 16T(-) and 35T(-) cells induced the expression of intercellular adhesion molecule-1 (ICAM-1) and HLA-DR by cultured keratinocytes. This response was inhibited by pretreatment of the supernatant with anti-IFN-gamma antibodies. These results indicate that some HTLV-1-infected T-cell lines constitutively secrete various cytokines, including biologically active IFN-gamma. The diversity of immunobiological functions of the T-cell lines may be related to the variety of clinical features present in ATL patients.
Department of Dermatology, Fukushima Medical College, Japan.
K
K
Iwatsuki
H
H
Harada
Y
Y
Motoki
F
F
Kaneko
F
F
Jin
M
M
Takigawa
D000328:Adult; D000956:Antigens, Viral / Q000032:analysis; D001483:Base Sequence; D002460:Cell Line; D017077:Culture Media, Conditioned / Q000494:pharmacology; D016207:Cytokines / Q000032:analysis; D017931:DNA Primers / Q000235:genetics; D004279:DNA, Viral / Q000032:analysis; D006684:HLA-DR Antigens / Q000378:metabolism; D015368:Human T-lymphotropic virus 1 / Q000235:genetics / Q000276:immunology; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D015603:Keratinocytes / Q000378:metabolism; D015458:Leukemia, T-Cell / Q000276:immunology* / Q000821:virology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis; D013601:T-Lymphocytes / Q000276:immunology*
D000956:Antigens, Viral; D017077:Culture Media, Conditioned; D016207:Cytokines; D017931:DNA Primers; D004279:DNA, Viral; D006684:HLA-DR Antigens; D012333:RNA, Messenger; D018799:Intercellular Adhesion Molecule-1