T4 DNA ligase modulates chromosome damage induced by restriction endonucleases through an error-free process.
The possible modulation by T4 DNA ligase of the DNA double-strand breaks produced by restriction endonucleases in living mammalian cells was studied. A clear decrease in the frequency of chromosomal aberrations was observed when T4 DNA ligase was included in the electroporation treatment along with restriction enzymes inducing either cohesive- or blunt-ends in DNA. The relative proportions of exchange-type aberrations were similar for both kinds of treatments (with and without ligase), which seems to suggest an error-free ligation by T4 DNA ligase.
Department of Cell Biology, Faculty of Biology, University of Sevilla, Spain.
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Piñero
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D000818:Animals; D016466:CHO Cells; D002869:Chromosome Aberrations; D002875:Chromosomes / Q000187:drug effects* / Q000648:ultrastructure; D006224:Cricetinae; D003412:Cricetulus; D004249:DNA Damage; D011088:DNA Ligases / Q000494:pharmacology*; D004260:DNA Repair / Q000502:physiology*; D004262:DNA Restriction Enzymes / Q000494:pharmacology*; D018274:Electroporation; D009153:Mutagens / Q000494:pharmacology*
D009153:Mutagens; D004262:DNA Restriction Enzymes; D011088:DNA Ligases