Effects of cadmium and of YAP1 and CAD1/YAP2 genes on iron metabolism in the yeast Saccharomyces cerevisiae.
Saccharomyces cerevisiae was more resistant to cadmium when the growth medium contained excess iron. Cadmium reduced the amount of iron taken up by cells during growth, and the cell ferrireductase activity was also strongly inhibited. These effects depended on the YAP1 and CAD1/YAP2 gene dosage. The growth rate of cells in iron-deficient conditions and their ferrireductase activity in the absence of added cadmium were also strongly affected by the dosage of YAP1 and CAD1/YAP2 genes. Our results suggest an indirect influence of these genes on iron metabolism, possibly via modification of the cell redox status.
Laboratoire de Biochimie des Porphyrines, Institut J. Monod, Université Paris, France.
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D000429:Alcohol Oxidoreductases / Q000235:genetics; D001483:Base Sequence; D002104:Cadmium / Q000633:toxicity*; D004271:DNA, Fungal / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics; D038181:FMN Reductase; D005800:Genes, Fungal; D007501:Iron / Q000378:metabolism* / Q000494:pharmacology; D008969:Molecular Sequence Data; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism; D010084:Oxidation-Reduction; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors / Q000235:genetics
C082904:CAD1 protein, S cerevisiae; D004271:DNA, Fungal; D004268:DNA-Binding Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; C086677:YAP1 protein, S cerevisiae; D002104:Cadmium; D007501:Iron; D000429:Alcohol Oxidoreductases; C018656:cinnamyl alcohol dehydrogenase; D038181:FMN Reductase; D009247:NADH, NADPH Oxidoreductases; C023903:ferric citrate iron reductase