Determination of the structure of the exopolysaccharide produced by Lactobacillus sake 0-1.
The exopolysaccharide produced by Lactobacillus sake 0-1 in a semi-defined medium was found to have an average molecular mass of 6 x 10(6) Da and a composition of D-glucose, L-rhamnose, and sn-glycerol 3-phosphate (3:2:1). The polysaccharide is partially O-acetylated. By means of partial acid hydrolysis, O-deacetylation, deglycerophosphorylation, methylation analysis, and 1D/2D NMR (1H, 13C, and 31P) studies the polysaccharide was shown to be composed of repeating units with the following structure: [formula: see text]
Bijvoet Center, Department of Bio-Organic Chemistry, Utrecht University, The Netherlands.
G W
GW
Robijn
D J
DJ
van den Berg
H
H
Haas
J P
JP
Kamerling
J F
JF
Vliegenthart
D002240:Carbohydrate Sequence; D003470:Culture Media / Q000737:chemistry; D006868:Hydrolysis; D007778:Lactobacillus / Q000737:chemistry* / Q000254:growth & development / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D015394:Molecular Structure; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis* / Q000737:chemistry* / Q000302:isolation & purification
D003470:Culture Media; D011135:Polysaccharides, Bacterial