A rapid method for detecting and mapping in vitro transcripts from supercoiled templates using endogenous RNase H.
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Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill 27599, USA.
Marzluff
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D000818:Animals; D001345:Autoradiography / Q000379:methods; D002467:Cell Nucleus / Q000378:metabolism; D004278:DNA, Superhelical / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D006657:Histones / Q000096:biosynthesis / Q000235:genetics; D051379:Mice; D009101:Multiple Myeloma; D010761:Phosphorus Radioisotopes; D011401:Promoter Regions, Genetic; D012313:RNA / Q000032:analysis / Q000096:biosynthesis*; D016914:Ribonuclease H / Q000378:metabolism*; D013698:Templates, Genetic; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured; D014544:Uridine Triphosphate / Q000378:metabolism
D004278:DNA, Superhelical; D006657:Histones; D010761:Phosphorus Radioisotopes; D012313:RNA; D016914:Ribonuclease H; D014544:Uridine Triphosphate