Molecular cloning of the GTP-cyclohydrolase structural gene RIB1 of Pichia guilliermondii involved in riboflavin biosynthesis.
The structural gene of GTP-cyclohydrolase, involved in riboflavin biosynthesis, was cloned from a Pichia guilliermondii genomic library. A 1855 bp genomic DNA fragment complementing the riboflavin auxotrophies of an Escherichia coli ribA mutant, defective in GTP-cyclohydrolase II, and a P. guilliermondii rib1 mutant was isolated and sequenced. An open reading frame with the potential to encode a protein of 344 amino acids with a predicted molecular mass of 38,711 Da was detected. The P. guilliermondii enzyme shows a high degree of homology to GTP-cyclohydrolases type II from E. coli and Baccillus subtilis and to GTP-cyclohydrolase from Saccharomyces cerevisiae. Functional GTP-cyclohydrolase from P. guilliermondii may consist of four identical subunits.
Liauta-Teglivets
O
O
Division of Cell Regulatory Systems, Academy of Sciences of Ukraine, Lviv, Ukraine.
Hasslacher
M
M
Boretskii
Iu R
IuR
Kohlwein
S D
SD
Shavlovskii
G M
GM
D000595:Amino Acid Sequence; D001407:Bacillus / Q000201:enzymology / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D006136:GTP Cyclohydrolase / Q000235:genetics*; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D010843:Pichia / Q000201:enzymology* / Q000235:genetics* / Q000378:metabolism; D012256:Riboflavin / Q000096:biosynthesis*; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics; D017386:Sequence Homology, Amino Acid
D004271:DNA, Fungal; D006136:GTP Cyclohydrolase; D012256:Riboflavin