Role of herpes simplex virus infection in the pathogenesis of facial paralysis in mice.
To clarify the role and site of herpes simplex virus (HSV) infection in the pathogenesis of facial paralysis, we examined the viral genome by the polymerase chain reaction and the neutralization antibody titer using microplates in an animal model. Following inoculation with HSV type 1 of the KOS strain into mouse auricles, HSV DNA appeared in the ipsilateral facial nerve on the 3rd day, and in bilateral facial nerves and the brain stem on the 10th day only in animals with facial paralysis. In animals without facial paralysis, no HSV DNA was detected in these tissues. The neutralization antibody titer was elevated between 4 and 20 days in all animals, with or without facial paralysis. Facial paralysis developed only on the inoculated side, even though HSV DNA was also present in the contralateral facial nerve. We conclude that HSV infection in the facial nerve and brain stem is prerequisite for facial paralysis, and suggest that an immunologic reaction following viral infection plays a key role in the pathogenesis.
Department of Otolaryngology, Ehime University School of Medicine, Japan.
S
S
Murakami
N
N
Hato
M
M
Mizobuchi
T
T
Doi
N
N
Yanagihara
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D001483:Base Sequence; D001933:Brain Stem / Q000821:virology; D004279:DNA, Viral / Q000032:analysis; D005154:Facial Nerve / Q000821:virology; D005158:Facial Paralysis / Q000209:etiology*; D005260:Female; D006561:Herpes Simplex / Q000150:complications* / Q000276:immunology / Q000821:virology; D018259:Herpesvirus 1, Human / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction
D000914:Antibodies, Viral; D004279:DNA, Viral