Stimulation of spinal delta-opioid receptors in mice selectively enhances the attenuation of delta-opioid receptor-mediated antinociception by antisense oligodeoxynucleotide.
Intrathecal (i.t.) pretreatment of male ICR mice with antisense oligodeoxynucleotide to delta-opioid receptor mRNA once a day for 1-3 days caused a time-dependent attenuation of i.t. administered [D-Ala2]deltorphin II-induced antinociception as measured by the tail-flick test. The attenuation of the antinociception induced by i.t. administered [D-Ala2]deltorphin II, a delta-opioid receptor agonist, was enhanced by i.t. pretreatment for 1 day with [D-Ala2]deltorphin II, but not [D-Ala2,N MePhe4,Gly(ol)5]enkephalin (DAMGO), a mu-opioid receptor agonist, or U50,488H, a kappa-opioid receptor agonist, given together with antisense oligodeoxynucleotide to delta-opioid receptor mRNA. The present results indicate that stimulation of spinal delta-opioid receptors by i.t. injection of [D-Ala2]deltorphin II selectively causes a loss of delta-opioid receptor-mediated antinociception in mice pretreated with antisense oligodeoxynucleotide to delta-opioid receptor mRNA.
Department of Anesthesiology, Medical College of Wisconsin, Milwaukee 53226, USA.
M
M
Narita
L F
LF
Tseng
D000700:Analgesics / Q000494:pharmacology*; D000818:Animals; D001483:Base Sequence; D007278:Injections, Spinal; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D008969:Molecular Sequence Data; D016376:Oligonucleotides, Antisense / Q000494:pharmacology*; D009842:Oligopeptides / Q000494:pharmacology; D010147:Pain Measurement / Q000187:drug effects; D012333:RNA, Messenger / Q000096:biosynthesis; D017465:Receptors, Opioid, delta / Q000819:agonists* / Q000096:biosynthesis / Q000502:physiology; D017473:Receptors, Opioid, kappa / Q000819:agonists; D017450:Receptors, Opioid, mu / Q000819:agonists; D013116:Spinal Cord / Q000187:drug effects / Q000378:metabolism*
D000700:Analgesics; D016376:Oligonucleotides, Antisense; D009842:Oligopeptides; D012333:RNA, Messenger; D017465:Receptors, Opioid, delta; D017473:Receptors, Opioid, kappa; D017450:Receptors, Opioid, mu; C060208:deltorphin I, Ala(2)-