A calcium-permeable cGMP-activated cation conductance in hippocampal neurons.
Whole-cell patch clamp recordings detected a previously unidentified cGMP-activated membrane conductance in cultured rat hippocampal neurons. This conductance is nonselectively permeable for cations and is completely but reversibly blocked by external Cd2+. The Ca2+ permeability of the hippocampal cGMP-activated conductance was examined in detail, indicating that the underlying ion channels display a high relative permeability for Ca2+. The results indicate that hippocampal neurons contain a cGMP-activated membrane conductance that has some properties similar to the cyclic nucleotide-gated channels previously shown in sensory receptor cells and retinal neurons. In hippocampal neurons this conductance similarly could mediate membrane depolarization and Ca2+ fluxes in response to intracellular cGMP elevation.
Leinders-Zufall
T
T
Section of Neurobiology, Yale University School of Medicine, New Haven, CT 06510, USA.
Rosenboom
H
H
Barnstable
C J
CJ
Shepherd
G M
GM
Zufall
F
F
D000818:Animals; D002104:Cadmium / Q000494:pharmacology; D002118:Calcium / Q000493:pharmacokinetics*; D002413:Cations, Divalent / Q000494:pharmacology; D002463:Cell Membrane Permeability / Q000187:drug effects*; D006152:Cyclic GMP / Q000031:analogs & derivatives / Q000494:pharmacology*; D006624:Hippocampus / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D016018:Least-Squares Analysis; D009431:Neural Conduction / Q000187:drug effects*; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D018408:Patch-Clamp Techniques; D051381:Rats
D002413:Cations, Divalent; D002104:Cadmium; C016276:8-bromocyclic GMP; D006152:Cyclic GMP; D002118:Calcium