In vitro action of continuous-wave ultrasound combined with adriamycin, X rays or hyperthermia.
We compared the ability of continuous-wave ultrasound to enhance cytotoxicity from X irradiation, hyperthermia or exposure to adriamycin. The survival of CHO cells exposed in culture medium to these agents was determined with and without continuous-wave ultrasound (1.62 or 1.765 MHz). In water-filled transmission exposure vessels with 2-cm-diameter Mylar end windows, 10-min insonation not producing cytotoxicity could produce .OH radicals (measured by electron paramagnetic resonance) even at 0.4 W/cm2. Ultrasound at intensities ranging between 1 and 2.5 W/cm2 increased the clonogenic cytotoxicity of adriamycin (P = 0.0023 by paired t test) but not of X rays (2-10 Gy) or hyperthermia (44 degrees C for 10-50 min). The only significant action of continuous-wave ultrasound under similar test conditions was the potentiation of adriamycin-induced clonogenic cytotoxicity, possibly mediated by cavitational activity.
Department of Radiation Oncology, University of Maryland School of Medicine, Baltimore 21201, USA.
G H
GH
Harrison
E K
EK
Balcer-Kubiczek
P L
PL
Gutierrez
D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000633:toxicity*; D016466:CHO Cells; D002470:Cell Survival / Q000187:drug effects* / Q000502:physiology / Q000528:radiation effects*; D006224:Cricetinae; D004307:Dose-Response Relationship, Radiation; D004317:Doxorubicin / Q000633:toxicity*; D004578:Electron Spin Resonance Spectroscopy; D006358:Hot Temperature; D017665:Hydroxyl Radical / Q000032:analysis / Q000378:metabolism; D006979:Hyperthermia, Induced; D007700:Kinetics; D014463:Ultrasonography; D014965:X-Rays
D000903:Antibiotics, Antineoplastic; D017665:Hydroxyl Radical; D004317:Doxorubicin