Karyotype analysis of anuran trypanosomes by pulsed-field gradient gel electrophoresis.
The chromosomes of 12 species and isolates of anuran trypanosomes were investigated by pulsed-field gradient gel electrophoresis. Twelve to 16 chromosomes ranging from 0.45 to 2.2 megabase pairs were found in each of these trypanosomes. Minichromosomes were not observed in any of the examined isolates. Results indicate that different species of anuran trypanosomes display distinct karyotype patterns, and that isolates from the same region are similar. Our findings also reveal that most chromosome profiles of these trypanosomes are in accordance with isoenzyme and random amplified polymorphic DNA analysis data.
Department of Zoology, University of Toronto, Ontario, Canada.
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D000818:Animals; D001001:Anura / Q000469:parasitology*; D002875:Chromosomes / Q000145:classification*; D016521:Electrophoresis, Gel, Pulsed-Field; D007621:Karyotyping; D014345:Trypanosoma / Q000235:genetics*