Quantitative analysis of the olfactory pathway for drug delivery to the brain.
Following intranasal administration to rats, wheat germ agglutinin-horseradish peroxidase (WGA-HRP) concentrated in the olfactory nerve and glomerular layers of the olfactory bulb resulting in a mean olfactory bulb concentration of 140 nM. A negligible amount of label was detected in the olfactory bulb following intravenous administration of WGA-HRP or intranasal administration of unconjugated HRP. This is the first quantitative assessment of intraneuronal transport of a protein into the brain using the olfactory route.
Thorne
R G
RG
Department of Neurology, Ramsey Clinic/St. Paul Ramsey Medical Center, MN 55101, USA.
Emory
C R
CR
Ala
T A
TA
Frey
W H
WH
D000281:Administration, Intranasal; D000818:Animals; D001921:Brain / Q000502:physiology*; D007150:Immunohistochemistry; D007275:Injections, Intravenous; D008297:Male; D009830:Olfactory Bulb / Q000033:anatomy & histology / Q000378:metabolism; D009831:Olfactory Mucosa / Q000294:innervation; D009833:Olfactory Pathways / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D018998:Wheat Germ Agglutinin-Horseradish Peroxidase Conjugate
D018998:Wheat Germ Agglutinin-Horseradish Peroxidase Conjugate