Retrospective investigation of hematopoietic chimerism after BMT by PCR amplification of hypervariable DNA regions.
We report on nine patients submitted to BMT with sex-matched donors and investigated by means of PCR amplification of the VNTRs ApoB, D1S80, DXS52, and D17S5. In all cases it was possible to detect a polymorphism able to distinguish between donor and patient cells, thus allowing us to recognize the presence of complete or mixed chimerism. In eight patients PCR analysis showed a complete chimerism during the entire follow-up. Only one of these patients relapsed, while the others are alive and without any sign of relapse 56.2 months (mean) after BMT. Mixed chimerism was detected in only one patient, who relapsed 3 months after this finding. These results confirm the usefulness of the study of PCR-amplified VNTRs in the assessment of marrow engraftment after BMT, mostly in sex-matched transplants where, in the absence of specific chromosome rearrangements, cytogenetic or FISH analysis cannot be used.
Istituto di Citomorfologia Umana Normale e Patologica, C.N.R., Chieti, Italy.
L
L
Stuppia
G
G
Calabrese
P
P
Di Bartolomeo
R
R
Peila
P G
PG
Franchi
E
E
Morizio
G
G
Palka
D000293:Adolescent; D000328:Adult; D001483:Base Sequence; D016026:Bone Marrow Transplantation; D002648:Child; D002678:Chimera; D004247:DNA / Q000235:genetics*; D005199:Fanconi Anemia / Q000628:therapy; D005260:Female; D006086:Graft vs Host Disease / Q000628:therapy; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000628:therapy; D015470:Leukemia, Myeloid, Acute / Q000628:therapy; D008297:Male; D018598:Minisatellite Repeats; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D016133:Polymerase Chain Reaction; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000628:therapy; D012189:Retrospective Studies; D014184:Transplantation, Homologous
D009838:Oligodeoxyribonucleotides; D004247:DNA