Anesthetics decreased the microdialysis extraction fraction of norepinephrine but not dopamine in the medial prefrontal cortex.
We evaluated the effects of chloral hydrate and pentobarbital sodium on the basal extracellular concentrations of dopamine (DA) and norepinephrine (NA) as well as their in vivo extraction fraction (relative recovery) at the medial prefrontal cortex (mPFC) in rats by using zero-net flux microdialysis method. Adult, male Sprague-Dawley rats anesthetized with either chloral hydrate (400 mg/kg, i.p., with 80 mg/kg i.v. supplements) or pentobarbital sodium (50 mg/kg, i.p., with 10 mg/kg i.v. supplements) were used as treatment groups. Conscious rats were used as a control group. The basal extracellular concentration and in vivo recovery of DA in the conscious group were 2.38 +/- 0.70 nM and 41 +/- 6%. In comparison with the chloral hydrate group (1.51 +/- 0.55 nM and 41 +/- 9%) and the pentobarbital sodium group (2.81 +/- 1.20 nM and 42 +/- 4%), there were no significant effects of anesthesia on the basal extracellular concentration and the in vivo recovery of DA at the mPFC. Additionally, the basal extracellular concentration and the in vivo recovery of NA in the conscious group were 1.59 +/- 0.37 nM and 51 +/- 8%. There also were no significant differences of the basal extracellular concentration of NA among these three groups (chloral hydrate group: 4.38 +/- 1.39 nM; pentobarbital group: 3.67 +/- 0.90 nM). However, the conscious group had a higher in vivo recovery than the two anesthetized groups (chloral hydrate group: 16 +/- 2%; pentobarbital group: 27 +/- 5%).(ABSTRACT TRUNCATED AT 250 WORDS)
Institute of Pharmacology, School of Life Sciences, National Yang-Ming University, Taipei, Taiwan, Republic of China.
W H
WH
Pan
Y J
YJ
Lai
D000777:Anesthetics / Q000494:pharmacology*; D000818:Animals; D004298:Dopamine / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D008297:Male; D017551:Microdialysis; D009638:Norepinephrine / Q000378:metabolism*; D010424:Pentobarbital / Q000494:pharmacology; D017397:Prefrontal Cortex / Q000187:drug effects* / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
D000777:Anesthetics; D010424:Pentobarbital; D004298:Dopamine; D009638:Norepinephrine