Characterization of the Pseudomonas aeruginosa transposable bacteriophage D3112 A and B genes.
The left end DNA of Mu-like transposable bacteriophage D3112 was sequenced from bp 2521 to bp 5483. Two large open reading frames were identified: ORF A (bp 2539-4611) and ORF B (bp 4626-5378). ORF A can encode a 690 amino acid, 78 kDa protein which is 44.4% similar to Mu transposase and ORF B can encode a 250 amino acid, 27 kDa protein, which is 46.4% similar to, though 62 amino acids shorter than, the Mu B protein. The cloned D3112 A gene exhibited activity on a mini-D3112-containing plasmid in Pseudomonas aeruginosa.
Department of Microbiology and Immunology, McGill University, Montréal, Québec, Canada.
P I
PI
Ulycznyj
K A
KA
Salmon
H
H
Douillard
M S
MS
DuBow
D000595:Amino Acid Sequence; D001435:Bacteriophages / Q000235:genetics*; D001483:Base Sequence; D004247:DNA / Q000737:chemistry; D004254:DNA Nucleotidyltransferases / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010957:Plasmids; D011550:Pseudomonas aeruginosa / Q000235:genetics*; D019895:Transposases; D014764:Viral Proteins / Q000235:genetics
D004268:DNA-Binding Proteins; C069215:MuB protein, Enterobacteria phage Mu; D014764:Viral Proteins; D004247:DNA; D004254:DNA Nucleotidyltransferases; D019895:Transposases