Crystallization and preliminary crystallographic analysis of recombinant abrin-a A-chain with ribosome inactivating activity.
Crystals have been obtained for a recombinant abrin-a A-chain produced by E. coli. The crystals were grown using PEG6000 as the precipitating agent. The crystals belong to an orthrhombic space group (P2(1)2(1)2(1)) and diffract to 1.7 A.
Kohno
T
T
Department of BioEngineering, Nagaoka University of Technology, Niigata, Japan.
Senda
T
T
Narumi
H
H
Kimura
S
S
Mitsui
Y
Y
D000036:Abrin / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D003460:Crystallization; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry* / Q000302:isolation & purification; D012270:Ribosomes / Q000187:drug effects / Q000378:metabolism*; D013329:Structure-Activity Relationship
D011994:Recombinant Proteins; D000036:Abrin