Cloning and sequencing of V genes from anti-osteosarcoma monoclonal antibodies TP-1 and TP-3: location of lysine residues and implications for radiolabeling.
Monoclonal antibodies TP-1 and TP-3 are of potential utility for the radioimmunodiagnosis of osteosarcoma in both human and canine patients. The V genes of these antibodies were cloned and sequenced and to facilitate radiolabeling of these proteins, the location of the lysine residues within these sequences have been determined. The V-domains of TP-1 contain a total of 12 lysines, 10 in the framework region and 2 in the CDR region, while the V-domains of TP-3 contain a total of 14 lysines, 11 in the framework region and 3 in the CDR regions. Using space-filling models, the availability of each lysine residue for radiolabeling, and potential interference with antigen binding was predicted.
Olafsen
T
T
Division of Molecular and Cell Biology, University of Oslo, Norway.
Bruland
O S
OS
Zalutsky
M R
MR
Sandlie
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I
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000737:chemistry; D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods; D018360:Crystallography, X-Ray; D017931:DNA Primers; D004283:Dog Diseases; D004285:Dogs; D005803:Genes, Immunoglobulin; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000096:biosynthesis / Q000737:chemistry; D007147:Immunoglobulin Light Chains / Q000096:biosynthesis / Q000737:chemistry; D007135:Immunoglobulin Variable Region / Q000096:biosynthesis* / Q000737:chemistry; D008239:Lysine; D008958:Models, Molecular; D008969:Molecular Sequence Data; D012516:Osteosarcoma / Q000000981:diagnostic imaging* / Q000276:immunology* / Q000662:veterinary; D016133:Polymerase Chain Reaction; D011487:Protein Conformation; D016719:Radioimmunodetection
D000911:Antibodies, Monoclonal; D017931:DNA Primers; D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D007135:Immunoglobulin Variable Region; D008239:Lysine