Regulation of the expression of cathepsin B in Sarcophaga peregrina (flesh fly) at the translational level during metamorphosis.
Previously, we reported that cathepsin B of pupal hemocytes participates in dissociation of the fat body of Sarcophaga larvae at metamorphosis. In this study, we demonstrated that the amount of cathepsin B in hemocytes was controlled at the translational level, i.e. larval hemocytes stored a significant amount of untranslated cathepsin B mRNA. When the larvae pupated, translation of the mRNA commenced, resulting in accumulation of cathepsin B. Our results indicate that the 3'-untranslated region (3'-UTR) of this mRNA is necessary for the repression of its translation in a rabbit reticulocyte lysate. We detected the 3'-UTR binding protein in the lysate of larval hemocytes, but not in the pupal hemocytes. The role of this protein in the translation of cathepsin B mRNA is discussed.
Faculty of Pharmaceutical Sciences, University of Tokyo, Japan.
T
T
Yano
N
N
Takahashi
S
S
Kurata
S
S
Natori
D000818:Animals; D002401:Cathepsin B / Q000235:genetics*; D004175:Diptera / Q000201:enzymology / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D015971:Gene Expression Regulation, Enzymologic; D006434:Hemocytes / Q000378:metabolism; D008675:Metamorphosis, Biological; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism
D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D002401:Cathepsin B