Recombinant enzymes for islet isolation: purification of a collagenase from Clostridium histolyticum and cloning/expression of the gene.
Hesse
F
F
Boehringer Mannheim GmbH, Penzberg, Germany.
Burtscher
H
H
Popp
F
F
Ambrosius
D
D
D000595:Amino Acid Sequence; D000818:Animals; D002469:Cell Separation / Q000379:methods*; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D003013:Clostridium / Q000201:enzymology*; D017364:Collagenases / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D005798:Genes, Bacterial; D007515:Islets of Langerhans / Q000166:cytology*; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000737:chemistry; D010957:Plasmids / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification; D013379:Substrate Specificity; D013552:Swine
D009842:Oligopeptides; D011994:Recombinant Proteins; C053633:phenylazobenzyloxycarbonyl-prolyl-leucyl-glycyl-prolyl-arginine; D017364:Collagenases