Characterization of the transcription start point of the trout estrogen receptor-encoding gene: evidence for alternative splicing in the 5' untranslated region.
The estrogen receptor (ER)-encoding gene (ER) regulates many genes implicated in the reproductive functions. Moreover, rainbow trout ER (rtER) is itself up-regulated by its own product. We have used Northern blot, RNase protection, primer extension and reverse transcription-polymerase chain reaction (RT-PCR) to study the position of the rtER mRNA transcription start point (tsp) in liver. This analysis has revealed the presence of a tsp positioned at the beginning of the cloned rtER cDNA. Functionality of this tsp was tested in transient transfections in CHO-K1 cells. The characterization of the rtER 5' untranslated region (UTR) showed that two transcripts exist in liver which differ in their 5'-UTR. The first one is 100% homologous to the cloned rtER cDNA sequence. The other one contains a 41-bp insertion. The isolation and sequencing of the first intron showed that this insertion arises from alternative splicing, due to the use of a splicing site internal to the first intron.
Lazennec
G
G
Laboratoire de Biologie Moléculaire, URA CNRS 256, Equipe associée d'Endocrinologie Moléculaire des Poissons, INRA/Université de Rennes 1, France.
Huignard
H
H
Valotaire
Y
Y
Kern
L
L
D017398:Alternative Splicing; D000818:Animals; D001483:Base Sequence; D016466:CHO Cells; D006224:Cricetinae; D008969:Molecular Sequence Data; D017686:Oncorhynchus mykiss / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D011960:Receptors, Estrogen / Q000235:genetics*; D014158:Transcription, Genetic; D014162:Transfection
D012333:RNA, Messenger; D011960:Receptors, Estrogen