Analysis of Ki-ras codon 12 mutations in the duodenal juice of patients with pancreatic cancer.
OBJECTIVE
Point mutations of the Ki-ras gene at codon 12 have been frequently identified in pure pancreatic juice of patients with pancreatic cancer in studies examining pancreatic cancer tissues. The aim of this study was to examine mutations of the Ki-ras codon 12 in the duodenal juice collected from patients with various pancreatic disorders.


METHODS
The duodenal juice was collected through a Dreiling tube installed in the duodenum during a secretin test. Analysis of the Ki-ras mutations was performed using the enriched polymerase chain reaction--single-strand conformation polymorphism technique.


RESULTS
Point mutations were detected in 12 of 19 patients with pancreatic cancer; of the 12 patients, 10 had ductal tubular adenocarcinoma and 2 intraductal papillary adenocarcinoma. Mutational patterns included GAT (n = 4), GTT (n = 3), CGT (n = 1), and double mutations of GTT and GAT (n = 3) and GAT and CGT (n = 1). In 41 patients with benign pancreatic disorders, a point mutation was detected in only 1 patient with chronic pancreatitis.


CONCLUSIONS
Analysis of the Ki-ras codon 12 mutations in the duodenal juice is useful in the diagnosis of pancreatic cancer.
Iguchi
H
H
Department of Biochemistry, National Kyushu Cancer Center, Fukuoka, Japan.
Sugano
K
K
Fukayama
N
N
Ohkura
H
H
Sadamoto
K
K
Ohkoshi
K
K
Seo
Y
Y
Tomoda
H
H
Funakoshi
A
A
Wakasugi
H
H
D001483:Base Sequence; D001826:Body Fluids / Q000502:physiology; D018270:Carcinoma, Ductal, Breast / Q000235:genetics / Q000378:metabolism; D002285:Carcinoma, Intraductal, Noninfiltrating / Q000235:genetics / Q000378:metabolism; D003062:Codon; D004386:Duodenum / Q000378:metabolism*; D011905:Genes, ras; D006801:Humans; D015335:Molecular Probes / Q000235:genetics; D008969:Molecular Sequence Data; D010182:Pancreatic Diseases / Q000235:genetics; D010190:Pancreatic Neoplasms / Q000235:genetics* / Q000378:metabolism*; D017354:Point Mutation
D003062:Codon; D015335:Molecular Probes