Secretion of laminin alpha 2 chain in cerebrospinal fluid.
The absence of laminin alpha 2 chain causes muscle cell degeneration and peripheral dysmyelination in congenital muscular dystrophy patients and dy mice, suggesting its role in the maintenance of sarcolemmal architecture and peripheral myelinogenesis. Here we demonstrate the secretion of laminin alpha 2 chain in cerebrospinal fluid (CSF). Laminin alpha 2 chain was detected as a minor component of the total CSF proteins or glycoproteins. Laminin alpha 2 chain was localized in the cytoplasm of epithelial cells of choroid plexus, suggesting active secretion. Our results suggest that immunochemical analysis of CSF laminin alpha 2 chain could be useful as an aid for the diagnosis of congenital muscular dystrophy.
Department of Neurology and Neuroscience, Teikyo University School of Medicine, Tokyo, Japan.
H
H
Yamada
H
H
Hori
T
T
Tanaka
S
S
Fujita
H
H
Fukuta-Ohi
S
S
Hojo
A
A
Tamura
T
T
Shimizu
K
K
Matsumura
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002417:Cattle; D002831:Choroid Plexus / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D015151:Immunoblotting; D007150:Immunohistochemistry; D007797:Laminin / Q000134:cerebrospinal fluid*; D009136:Muscular Dystrophies / Q000134:cerebrospinal fluid / Q000151:congenital / Q000175:diagnosis; D013126:Spinal Nerve Roots / Q000737:chemistry
D000911:Antibodies, Monoclonal; D007797:Laminin