Structure and organization of the human glucose phosphate isomerase gene (GPI).
Two overlapping yeast artificial chromosome clones containing the human glucose 6-phosphate isomerase gene (GPI) have been isolated. PCR and direct sequencing were used to determine the exon/intron structure of the gene. The gene spans in excess of 40 kb and consists of 18 exons ranging in size from 44 to 153 bp. All splice sites conform to the GT/AG rule.
Wellcome Research Laboratory for Molecular Genetics, Division of Biochemistry and Molecular Biology, UMDS, Guy's Hospital, London, United Kingdom.
J I
JI
Walker
M J
MJ
Morgan
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Faik
D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D018244:Chromosomes, Artificial, Yeast; D003001:Cloning, Molecular; D017931:DNA Primers; D005091:Exons; D015698:Genomic Library; D005956:Glucose-6-Phosphate Isomerase / Q000235:genetics*; D015186:Hominidae / Q000235:genetics*; D006801:Humans; D007438:Introns; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping
D017931:DNA Primers; D005956:Glucose-6-Phosphate Isomerase