A microfluorometric method for measuring ethoxycoumarin-O-deethylase activity on individual Drosophila melanogaster abdomens: interest for screening resistance in insect populations.
We developed a method for measuring ethoxycoumarin deethylase (ECOD) activity using a single Drosophila abdomen. The activities obtained were well correlated with the classic method from microsomes (r = 0.902). This new method, performed in microtitration plates, was at least six times more sensitive compared to the conventional cuvet fluorometric one. Moreover, it was possible among a large number of insects to differentiate those with low or high ECOD activities. This improved procedure has been checked upon crosses between resistant strain (with high ECOD activity) and susceptible strain (with low ECOD activity). The results demonstrate the possible separation of resistant phenotypes and emphasize the importance of this approach in assessing the spreading of insecticide resistance in natural populations of insects.
Institut National de la Recherche Agronomique, Equipe INSERM, Antibes, France.
G
G
de Sousa
A
A
Cuany
A
A
Brun
M
M
Amichot
R
R
Rahmani
J B
JB
Bergé
D015660:7-Alkoxycoumarin O-Dealkylase / Q000032:analysis*; D000005:Abdomen; D000818:Animals; D004331:Drosophila melanogaster / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D005069:Evaluation Studies as Topic; D007305:Insecticide Resistance / Q000235:genetics; D008861:Microsomes / Q000201:enzymology; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000295:instrumentation / Q000379:methods* / Q000706:statistics & numerical data
D015660:7-Alkoxycoumarin O-Dealkylase