Tobacco MAP kinase: a possible mediator in wound signal transduction pathways.
A complementary DNA encoding a mitogen-activated protein (MAP) kinase homolog has been isolated from tobacco plants. Transcripts of the corresponding gene were not observed in healthy tobacco leaves but began to accumulate 1 minute after mechanical wounding. In tobacco plants transformed with the cloned complementary DNA, trans inactivation of the endogenous homologous gene occurred, and both production of wound-induced jasmonic acid and accumulation of wound-inducible gene transcripts were inhibited. In contrast, the levels of salicylic acid and transcripts for pathogen-inducible, acidic pathogenesis-related proteins were increased upon wounding. These results indicate that this MAP kinase is part of the initial response of higher plants to mechanical wounding.
Institute of Applied Biochemistry, University of Tsukuba, Ibaraki, Japan.
S
S
Seo
M
M
Okamoto
H
H
Seto
K
K
Ishizuka
H
H
Sano
Y
Y
Ohashi
D000595:Amino Acid Sequence; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D003517:Cyclopentanes / Q000378:metabolism; D018076:DNA, Complementary / Q000235:genetics; D017343:Genes, Plant; D008969:Molecular Sequence Data; D054883:Oxylipins; D010741:Phospholipases A / Q000378:metabolism; D010766:Phosphorylation; D010940:Plant Proteins / Q000378:metabolism; D030821:Plants, Genetically Modified; D010947:Plants, Toxic; D012459:Salicylates / Q000378:metabolism; D020156:Salicylic Acid; D015398:Signal Transduction; D014026:Nicotiana / Q000201:enzymology* / Q000235:genetics
D003517:Cyclopentanes; D018076:DNA, Complementary; D054883:Oxylipins; D010940:Plant Proteins; D012459:Salicylates; C053376:pathogenesis-related proteins, plant; C011006:jasmonic acid; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D010741:Phospholipases A; D020156:Salicylic Acid