Reactive oxygen generation by azomethine H: a new antimalarial drug.
Superoxide radical (O2-) is a free radical that may be involved in various toxic processes. Cu-Zn superoxide dismutase catalyzes the dismutation of the superoxide free radical and protects cells from oxidative damage. A rat bioassay validated for the identification of the toxic effects of azomethine H revealed increased serum activities of amylase, alanine transaminase, and alkaline phosphatase. The lipoperoxide and bilirubin concentrations were also increased in animals that received azomethine H (1 g/kg) from ascorbic or hydrochloric acid solutions. Azomethine H increased Cu-Zn superoxide dismutase activity. This elevation of Cu-Zn superoxide dismutase activity was highest on the 7th day and was at levels comparable with those of control rats from day 60 onwards. Superoxide is an important intermediate in the action and toxicity of azomethine H.
Novelli
E L
EL
Departamento de Química, Universidade Estadual Paulista, UNESP, Botucatu, São Paülo, Brasil.
Silva
A M
AM
Novelli
J L
JL
Curi
P R
PR
D000818:Animals; D000962:Antimalarials / Q000494:pharmacology*; D004789:Enzyme Activation / Q000187:drug effects; D008054:Lipid Peroxides / Q000097:blood; D008297:Male; D009282:Naphthalenesulfonates / Q000494:pharmacology*; D010100:Oxygen / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000097:blood; D013882:Thiosemicarbazones / Q000494:pharmacology*; D013997:Time Factors
D000962:Antimalarials; D008054:Lipid Peroxides; D009282:Naphthalenesulfonates; D013882:Thiosemicarbazones; C097966:azomethine H; D013482:Superoxide Dismutase; D010100:Oxygen