A reassessment of Y chromosomal behaviour in germ cells and Sertoli cells of the mouse as revealed by in situ hybridisation.
In situ hybridisation experiments were carried out to reappraise the state of condensation of the Y chromosome in germ cells and Sertoli cells of the mouse. Previous work had suggested that all testicular cells showed a condensed Y chromosome prior to the adult stage. We now demonstrate that, although the Y chromosome is condensed in pre-pubertal Sertoli cells, it is greatly expanded in primordial germ cells (gonocytes). An expanded Y-signal is first seen in Sertoli cell nuclei at or around day 21 of postnatal development, coinciding with the first appearance of spermatids in the germinal epithelium.
Chandley
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MRC Human Genetics Unit, Western General Hospital, Edinburgh EH4 2XU, UK.
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D000818:Animals; D005854:Germ Cells / Q000648:ultrastructure*; D006720:Homozygote; D017404:In Situ Hybridization, Fluorescence; D007713:Klinefelter Syndrome / Q000662:veterinary; D008297:Male; D051379:Mice; D008817:Mice, Mutant Strains; D009154:Mutation; D012708:Sertoli Cells / Q000648:ultrastructure*; D013091:Spermatogenesis; D013234:Stem Cells / Q000648:ultrastructure*; D013737:Testis / Q000196:embryology / Q000254:growth & development*; D014998:Y Chromosome / Q000648:ultrastructure*