[Modification of DNA with 4-aminooxybutylamine as a method for obtaining highly-sensitive hybridization probes. Mapping of the gene for human chorionic somatomammotropin hormone].
A new method for preparation of highly sensitive nonradioactive probes for dot, dot-blot and in situ hybridization was developed. The method is based on chemical modification (transamination) of cytosine residues with 4-aminooxybutylamine following by coupling biotin or fluorescein to aliphatic aminogroups introduced into DNA. Such a probe have been used for detection of gene encoding chorionic somatomammotropin hormone (hCS) in genomic blot hybridization. The gene hCS was mapped using isotopic and nonisotopic in situ hybridization on human chromosome 17.
Adarichev
V A
VA
Vorob'eva
N V
NV
Grafodatskiĭ
A S
AS
Dymshits
G M
GM
Sablina
O V
OV
D002874:Chromosome Mapping; D002886:Chromosomes, Human, Pair 17; D004247:DNA / Q000737:chemistry*; D015342:DNA Probes / Q000138:chemical synthesis*; D006801:Humans; D006898:Hydroxylamines / Q000737:chemistry*; D017403:In Situ Hybridization; D010928:Placental Lactogen / Q000235:genetics*
D015342:DNA Probes; D006898:Hydroxylamines; D004247:DNA; D010928:Placental Lactogen
Modifikatsiia DNK 4-aminooksibutilaminom kak sposob polucheniia vysokochuvstvitel'nykh gibridizatsionnykh zondov. Lokalizatsiia gena khorionicheskogo somatomammotropnogo gormona cheloveka.