Identification of the S-Endo 1 endothelial-associated antigen.
We have recently described a monoclonal antibody, S-Endo 1, recognizing a molecule constitutively expressed in all types of human endothelial cells. We showed that this protein around 118 kDa and located at the endothelial cell-cell junction presented sequence identity with MUC18 described as a tumor marker in human melanoma. The difference in antibodies immunoreactivity and antigen molecular weight heterogeneity observed between various cell types strongly suggested S-Endo 1 antigen isoforms expression.
Bardin
N
N
Laboratoire d'Hématologie, Faculté de pharmacie, Marseille, France.
Francès
V
V
Lesaule
G
G
Horschowski
N
N
George
F
F
Sampol
J
J
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000954:Antigens, Surface / Q000032:analysis* / Q000737:chemistry; D015153:Blotting, Western; D051929:CD146 Antigen; D002460:Cell Line; D002478:Cells, Cultured; D003429:Cross Reactions; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006801:Humans; D007124:Immunoenzyme Techniques; D008562:Membrane Glycoproteins / Q000032:analysis / Q000737:chemistry; D008969:Molecular Sequence Data; D019006:Neural Cell Adhesion Molecules; D017386:Sequence Homology, Amino Acid; D014471:Umbilical Veins
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000954:Antigens, Surface; D051929:CD146 Antigen; C497902:MCAM protein, human; D008562:Membrane Glycoproteins; D019006:Neural Cell Adhesion Molecules