title: Field gradient techniques in NMR spectroscopy.
abstract: In recent years NMR spectroscopy has emerged as a powerful technique for studying the structure and dynamics of biomolecules in solution. The development of shielded pulsed-field gradient coils for high-resolution NMR spectroscopy has led to significant improvements in a large number of experiments which are used to provide such information. Experiments enhanced with pulsed-field gradients have fewer artifacts, suffer far less from problems of solvent suppression and have reduced phase cycles relative to their non-gradient counterparts. The whole array of NMR experiments available for macromolecular structure determination is likely to benefit substantially from the incorporation of gradient pulses.
authors.affiliation: Department of Medical Genetics, University of Toronto, Ontario, Canada.
authors.forename: L E
authors.initials: LE
authors.lastname: Kay
mesh_terms: D004058:Diffusion; D046911:Macromolecular Substances; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D011506:Proteins / Q000737:chemistry*; D012997:Solvents
chemical_list: D046911:Macromolecular Substances; D011506:Proteins; D012997:Solvents