Immunological localisation of melanocortin 1 receptor on the cell surface of WM266-4 human melanoma cells.
The localisation of melanocortin 1 receptor (MC1R) in WM266-4 human melanoma cells was investigated by applying an antipeptide antiserum specific for the cloned human MC1R (MSH receptor). In enzyme-linked immunosorbent assay (ELISA), the immunoreactivity was detected in the membrane fraction of WM266-4 cells. The ELISA reactivity could be inhibited by an antiserum pre-absorbed with its specific synthetic peptide. In immunocytochemistry, the specific immunoreactivity was demonstrated on the surface of the cells by using either biotin-avidin immunoalkaline phosphatase- or TRITC-staining method. These results indicate that the MC1R is prominently present on the plasma membrane of WM266-4 human melanoma cells.
Xia
Y
Y
Department of Pharmaceutical Biosciences, Uppsala University, Sweden.
Muceniece
R
R
Wikberg
J E
JE
D000345:Affinity Labels / Q000378:metabolism; D002462:Cell Membrane / Q000648:ultrastructure; D006801:Humans; D007150:Immunohistochemistry; D007457:Iodine Radioisotopes; D007700:Kinetics; D009074:Melanocyte-Stimulating Hormones / Q000378:metabolism; D008545:Melanoma / Q000378:metabolism / Q000648:ultrastructure*; D011869:Radioligand Assay; D011979:Receptors, Pituitary Hormone / Q000032:analysis* / Q000378:metabolism; D014407:Tumor Cells, Cultured; D000521:alpha-MSH / Q000031:analogs & derivatives / Q000378:metabolism
D000345:Affinity Labels; D007457:Iodine Radioisotopes; D011979:Receptors, Pituitary Hormone; D000521:alpha-MSH; C027756:MSH, 4-Nle-7-Phe-alpha-; C029446:MSH receptor; D009074:Melanocyte-Stimulating Hormones