Culturing satellite cells from living single muscle fiber explants.
Conventional methods for isolating myogenic (satellite) cells are inadequate when only small quantities of muscle, the tissue in which satellite cells reside, are available. We have developed a tissue culture system that reliably permits isolation of intact, living, single muscle fibers with associated satellite cells from predominantly fast and slow muscles of rat and mouse; maintenance of the isolated fibers in vitro; dissociation, proliferation, and differentiation of satellite cells from each fiber; and removal of the fiber from culture for analysis.
Rosenblatt
J D
JD
Muscle Cell Biology Group, Royal Postgraduate Medical School, Hammersmith Hospital, London, England.
Lunt
A I
AI
Parry
D J
DJ
Partridge
T A
TA
D000818:Animals; D018929:Cell Culture Techniques; D002469:Cell Separation / Q000379:methods; D005260:Female; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018101:Mice, Inbred mdx; D018485:Muscle Fibers, Skeletal / Q000166:cytology*; D051381:Rats; D017208:Rats, Wistar