title: Chemiluminescence detection of mono-, bis-, and tris-hydroperoxy triacylglycerols present in vegetable oils.
abstract: A reliable method was needed to analyze molecular species of oxidized vegetable oils. In order to accomplish this goal, mono-, bis-, and tris-hydroperoxides (Mono-OOH, Bis-OOH, and Tris-OOH, respectively) of triacylglycerols formed during autoxidation and photosensitized oxidation of oils were determined by reversed-phase high-performance liquid chromatography in combination with chemiluminescence detection (CL-HPLC). Mono-OOH was the major species (96% of total hydroperoxides) in trioleoylglycerol [peroxide value (PV) 0.16 meg/kg], and Bis-OOH and Tris-OOH showed prolonged accumulation with photooxidation. This profile was confirmed in photooxidation of trilinoleoylglycerol and trilinoleoylglycerol. Soybean oil (PV 6 meq/kg) contained Mono-OOH oleoyl-linoleoylglycerol as the main peroxidic molecular species (50% of total hydroperoxides). Mono-OOH trilinoleoylglycerol was the principal species (61% of total hydroperoxides) in safflower oil (PV 5 meq/kg), and Mono-OOH oleoyl-oleoyl-linoleoylglycerol was the representative species (66% of total hydroperoxides) in olive oil (PV 3 meq/kg). The CL-HPLC method, which is specific for the detection of hydroperoxides, should prove useful in studies of triacylglycerol oxidation in foods and vegetable oils.
authors.affiliation: Department of Applied Biological Chemistry, Tohoku University, Sendai, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Miyazawa
authors.forename: H
authors.initials: H
authors.lastname: Kunika
authors.forename: K
authors.initials: K
authors.lastname: Fujimoto
authors.forename: Y
authors.initials: Y
authors.lastname: Endo
authors.forename: T
authors.initials: T
authors.lastname: Kaneda
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D015227:Lipid Peroxidation; D008163:Luminescent Measurements; D010084:Oxidation-Reduction; D010545:Peroxides / Q000032:analysis*; D010777:Photochemistry; D010938:Plant Oils / Q000737:chemistry*; D016339:Spectrometry, Mass, Fast Atom Bombardment; D014280:Triglycerides / Q000032:analysis*
chemical_list: D010545:Peroxides; D010938:Plant Oils; D014280:Triglycerides