title: In vitro demonstration of a fundamental difference in the proliferation of murine and human bone marrow and lymphocytes following ultraviolet irradiation: relevance to bone marrow transplantation.
abstract: Exposure of rodent allogeneic donor marrow and splenocyte grafts to ultraviolet radiation (UVR) has been shown to permit durable engraftment at doses that abolish graft-versus-host disease (GVHD) and graft rejection. We have compared both murine and human alloreactive and mitogen-induced lymphoid responses and bone marrow proliferation in mixed lymphocyte culture (MLC), phytohemagglutinin (PHA)-induced proliferation and colony-forming unit-granulocyte/macrophage (CFU-GM) assays using germicidal UVC (200-290 nm), broadband and narrowband UVB (290-320 nm) and UVA (320-400 nm) sources. Our data show a wavelength and dose-dependent reduction in lymphoid proliferation in the mouse with CFU-GM survival of 50-75% of control at doses required to abolish allogeneic lymphocyte responses for all lamps. In contrast, human lymphocyte responses are more resistant to UVC with CFU-GM proliferation reduced to zero when allostimulation is abolished. Mitogen-induced lymphoid responses show a similar wavelength-dependent sensitivity. Abolition of response in MLC using UV-irradiated stimulator cells was less sensitive than proliferation with UV-irradiated responder cells at all wavelengths in both species. With all sources, murine CFU-GM proliferation is less susceptible to UVR than human marrow at doses required to abolish lymphoid responses.
authors.affiliation: Regional Transfusion Centre, Southmead, Bristol, UK.
authors.forename: J G
authors.initials: JG
authors.lastname: Hudson
authors.forename: R
authors.initials: R
authors.lastname: Pullens
authors.forename: V
authors.initials: V
authors.lastname: Godwin
authors.forename: A W
authors.initials: AW
authors.lastname: Preece
authors.forename: D H
authors.initials: DH
authors.lastname: Pamphilon
mesh_terms: D000818:Animals; D001853:Bone Marrow / Q000276:immunology; D001854:Bone Marrow Cells; D016026:Bone Marrow Transplantation / Q000276:immunology*; D002455:Cell Division / Q000528:radiation effects; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects / Q000528:radiation effects*; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology / Q000528:radiation effects*; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008934:Mitogens / Q000494:pharmacology; D014466:Ultraviolet Rays
chemical_list: D008934:Mitogens