title: Protein binding of sotalol enantiomers in young and elderly human and rat serum using ultrafiltration.
abstract: The protein binding of sotalol (STL) enantiomers was evaluated using an ultrafiltration technique with serum from young (32 +/- 2 years, n = 5) and elderly (73 +/- 6 years, n = 5) male and female humans, and young (8 weeks, n = 4) and elderly (60 weeks, n = 3) male Sprague-Dawley rats. Serum samples were collected and immediately frozen at -20 degrees C. Within 1 week, the serum samples were thawed at room temperature, and adjusted to pH 7.4 using 0.05 M phosphate buffer, pH 5.0. Aliquots were spiked with 250 ng mL-1 and 500 ng mL-1 of each STL enantiomer, placed in ultrafiltration sets (Microsep, 30K molecular weight cut-off), capped, equilibrated to 37 degrees C, and centrifuged at 1850g for 1.5 h at 37 degrees C. Aliquots of ultrafiltrate and unspun serum were analysed for STL enantiomer concentration using a stereospecific HPLC assay. In all groups, bound fraction was less than 7% for both STL enantiomers. There were no significant differences in bound fraction between groups, or between enantiomers. Adsorption of STL enantiomers to the ultrafiltration device and membrane, evaporative loss of serum samples during centrifugation, and protein concentration in each ultrafiltrate sample were all negligible. It is concluded that the binding of STL in human and rat serum at therapeutic concentrations and physiological temperature and pH is negligible and non-stereoselective.
authors.affiliation: Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, Canada.
authors.forename: R A
authors.initials: RA
authors.lastname: Carr
authors.forename: F M
authors.initials: FM
authors.lastname: Pasutto
authors.forename: R Z
authors.initials: RZ
authors.lastname: Lewanczuk
authors.forename: R T
authors.initials: RT
authors.lastname: Foster
mesh_terms: D000319:Adrenergic beta-Antagonists / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D000328:Adult; D000368:Aged; D000375:Aging / Q000378:metabolism*; D000704:Analysis of Variance; D000818:Animals; D001798:Blood Proteins / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006801:Humans; D008297:Male; D011485:Protein Binding; D051381:Rats; D017207:Rats, Sprague-Dawley; D013015:Sotalol / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D013045:Species Specificity; D013237:Stereoisomerism; D014462:Ultrafiltration
chemical_list: D000319:Adrenergic beta-Antagonists; D001798:Blood Proteins; D013015:Sotalol