Haematological, bone marrow and clinical chemical changes in neonatal foals given canine recombinant granulocyte-colony stimulating factor.
Five Thoroughbred and Quarter Horse cross foals were given 20 micrograms canine recombinant granulocyte-colony stimulating factor (rcG-CSF) per kg bwt intramuscularly (i.m.) on the day of birth and 10 micrograms rcG-CSF/kg for 13 additional days. During this time and for an additional 21 days haematology, bone marrow and clinical chemical analyses were performed. After one day of rcG-CSF administration leucocyte and neutrophil counts increased from 9.16 x 10(9)/l to 23.44 x 10(9)/l and from 6.45 x 10(9)/l to 19.61 x 10(9)/l, respectively. The counts continued to increase for the next 3-4 days and then there was a slight decrease. A second increase followed and the leucocyte and neutrophil counts increased to 52.84 x 10(9)/l and 45.16 x 10(9)/l on the day after the last rcG-CSF administration (Day 15). The counts decreased rapidly immediately after the administration of rcG-CSF was stopped and then at a slower rate. The cell counts were still higher than in the controls at the end of the study period (Day 35). Bone marrow cellularity increased from 10-25% before rcG-CSF was given to 60-80% after 5 days. The increase in cellularity was due to increased myeloid activity because the myeloid to erythroid ratio increased from 2.7 to 8.8. Serum chemistry changes were minimal although foals given rcG-CSF at various times had lower glucose concentrations and increased alkaline phosphatase and gamma glutamyl transferase activities.
Zinkl
J G
JG
Department of Clinical Pathology, School of Veterinary Medicine, University of California, Davis 95691, USA.
Madigan
J E
JE
Fridmann
D M
DM
Kabbur
M B
MB
Reynal-O'Connor
J
J
Andresen
J W
JW
D000469:Alkaline Phosphatase / Q000032:analysis / Q000097:blood; D000818:Animals; D000831:Animals, Newborn / Q000097:blood* / Q000378:metabolism; D001707:Biopsy, Needle; D001772:Blood Cell Count / Q000187:drug effects / Q000662:veterinary; D001774:Blood Chemical Analysis / Q000662:veterinary; D001786:Blood Glucose / Q000032:analysis; D001853:Bone Marrow / Q000187:drug effects* / Q000473:pathology; D004285:Dogs; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D006736:Horses / Q000097:blood* / Q000378:metabolism; D007958:Leukocyte Count / Q000187:drug effects / Q000662:veterinary; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects; D010976:Platelet Count / Q000187:drug effects / Q000662:veterinary; D011994:Recombinant Proteins / Q000494:pharmacology; D005723:gamma-Glutamyltransferase / Q000032:analysis / Q000097:blood
D001786:Blood Glucose; D011994:Recombinant Proteins; D016179:Granulocyte Colony-Stimulating Factor; D005723:gamma-Glutamyltransferase; D000469:Alkaline Phosphatase