Superactivity of peroxidase solubilized in reversed micellar systems.
Vaccinium mirtyllus peroxidase solubilized in reversed micelles was used for the oxidation of guaiacol. Some relevant parameters for the enzymatic activity, such as pH, w(o) (molar ratio water/surfactant), surfactant type and concentration, and cosurfactant concentration, were investigated. The peroxidase showed higher activities in reversed micelles than in aqueous solution. The stability of the peroxidase in reversed micelles was also studied, namely, the effect of w(o) and temperature on enzyme deactivation. The peroxidase displayed higher stabilities in CTAB/hexanol in isooctane reversed micelles, with half-life times higher than 500 h.
Setti
L
L
Instituto de Tecnologia e Química Biológica, Universidade Nova Lisboa, Oeiras, Portugal.
Fevereiro
P
P
Melo
E P
EP
Pifferi
P G
PG
Cabral
J M
JM
Aires-Barros
M R
MR
D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000737:chemistry; D003470:Culture Media; D003902:Detergents / Q000737:chemistry; D004795:Enzyme Stability / Q000502:physiology; D005638:Fruit / Q000201:enzymology; D006139:Guaiacol / Q000737:chemistry; D000441:Hexanols / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008823:Micelles; D009795:Octanes / Q000737:chemistry; D010084:Oxidation-Reduction; D010544:Peroxidases / Q000737:chemistry / Q000378:metabolism*; D012995:Solubility; D013501:Surface-Active Agents / Q000737:chemistry; D013696:Temperature
D002593:Cetrimonium Compounds; D003470:Culture Media; D003902:Detergents; D000441:Hexanols; D008823:Micelles; D009795:Octanes; D013501:Surface-Active Agents; C036260:1-hexanol; D006139:Guaiacol; D010544:Peroxidases; C045798:2,2,4-trimethylpentane; D000077286:Cetrimonium