Falsely elevated steroidal assay levels related to heterophile antibodies against various animal species.
Two cases of falsely elevated serum estradiol and 1 case of spuriously elevated serum progesterone performed on an automated immunoassay instrument are described. Further testing of same specimens indicated the presence of heterophilic antibodies against rabbit IgG and sheep IgG, respectively.
Check
J H
JH
University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School at Camden, Cooper Hospital/University Medical Center, USA.
Ubelacker
L
L
Lauer
C C
CC
D000328:Adult; D000818:Animals; D000910:Antibodies, Heterophile / Q000097:blood*; D000918:Antibody Specificity; D004797:Enzyme-Linked Immunosorbent Assay; D004958:Estradiol / Q000097:blood*; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D011374:Progesterone / Q000097:blood*; D011817:Rabbits; D011863:Radioimmunoassay; D012756:Sheep
D000910:Antibodies, Heterophile; D007074:Immunoglobulin G; D011374:Progesterone; D004958:Estradiol