title: Integrative and replicative transformation of Penicillium canescens with a heterologous nitrate-reductase gene.
abstract: A wild isolate of Penicillium canescens was subjected to mutagenesis, and 150 chlorate-resistant mutants were isolated and classified in respect of their ability to utilize various nitrogen sources. Strains supposedly deficient in nitrate reductase have been transformed with the nitrate-reductase gene from Aspergillus niger. Transformation probably occurred by non-homologous integration of the transforming vector into the chromosome. Co-transformation with the AMA 1 replicating element from A. nidulans enhanced transformation frequency up to 2000-fold, and was shown to result in autonomous maintenance of replicating concatenates, one of which was re-isolated by transformation of E. coli.
authors.affiliation: Genetics Laboratories, University of Glasgow, Scotland, UK.
authors.forename: A Y
authors.initials: AY
authors.lastname: Aleksenko
authors.forename: N A
authors.initials: NA
authors.lastname: Makarova
authors.forename: I V
authors.initials: IV
authors.lastname: Nikolaev
authors.forename: A J
authors.initials: AJ
authors.lastname: Clutterbuck
mesh_terms: D004271:DNA, Fungal; D016296:Mutagenesis; D050901:Nitrate Reductase; D009565:Nitrate Reductases / Q000235:genetics*; D009584:Nitrogen / Q000378:metabolism; D010407:Penicillium / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D014170:Transformation, Genetic
chemical_list: D004271:DNA, Fungal; D009565:Nitrate Reductases; D050901:Nitrate Reductase; D009584:Nitrogen