Heterogeneity of renal carcinoma.
Monoclonal antibodies were used to study the expression of three recently characterized basement membrane components and two carbohydrate antigens in 11 renal-cell carcinomas, using immunohistological and biochemical techniques. The expression of several site-specific kidney antigens in renal-cell carcinoma were studied to determine the origin of the carcinoma and if it is possible further classify this type of carcinoma. Tubulointerstitial nephritis antigen (TIN) and two alpha-chains of type IV collagen, alpha 1 (IV) and alpha 3 (IV) were studied. In addition the expression of carbohydrate antigens Lex and SLex, which also exhibit site-specific distribution were characterized. Lex and SLex antibodies stained the majority of the tumours. TIN was expressed in 9 of 11 tumours, the alpha 1 (IV) chain was present in all 11, and the alpha 3 (IV) chain in two of the 11 tumours. Interestingly, the two alpha 3 (IV)-positive tumours were the same two that were negative for TIN. In normal tissue alpha 3 (IV) is found in distal tubules while TIN is found in proximal tubules. Our results are consistent with earlier observations that the proximal tubule is the origin of most renal-cell carcinomas, but the results also indicate that renal-cell carcinoma may originate from the distal tubule.
Mårtensson
S
S
Department of Urology, University of Lund, Sweden.
Brunmark
C
C
Ohlsson
L
L
Bak-Jensen
E
E
Butkowski
R
R
Boketoft
A
A
Wieslander
J
J
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D002292:Carcinoma, Renal Cell / Q000276:immunology* / Q000378:metabolism*; D015815:Cell Adhesion Molecules / Q000378:metabolism; D003094:Collagen / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D007680:Kidney Neoplasms / Q000276:immunology* / Q000378:metabolism*; D007686:Kidney Tubules, Distal / Q000276:immunology / Q000378:metabolism; D007687:Kidney Tubules, Proximal / Q000276:immunology / Q000378:metabolism; D016256:Lewis X Antigen / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000378:metabolism; D009844:Oligosaccharides / Q000378:metabolism; D000080506:Sialyl Lewis X Antigen; D034501:Telomere-Binding Proteins
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D015815:Cell Adhesion Molecules; D016256:Lewis X Antigen; D008562:Membrane Glycoproteins; D009844:Oligosaccharides; D000080506:Sialyl Lewis X Antigen; C085434:TINAG protein, human; C440868:TINF2 protein, human; D034501:Telomere-Binding Proteins; D003094:Collagen