C-C chemokines, but not the C-X-C chemokines interleukin-8 and interferon-gamma inducible protein-10, stimulate transendothelial chemotaxis of T lymphocytes.
Eight chemokines were tested for ability to elicit transendothelial chemotaxis of unstimulated peripheral blood T lymphocytes. The C-C chemokines monocyte chemotactic protein (MCP)-2, MCP-3, RANTES, macrophage inflammatory protein (MIP)-1 alpha, MIP-1 beta, and, as previously described, MCP-1 induced significant, dose-dependent transendothelial chemotaxis of CD3+ T lymphocytes. In contrast, the C-X-C chemokines interleukin-8 (IL-8) and interferon-gamma inducible protein-10 (IP-10) failed to induce transendothelial chemotaxis of CD3+ T lymphocytes or T lymphocyte subsets. RANTES, MIP-1 alpha, and MIP-1 beta induced significant transendothelial chemotaxis of CD4+, CD8+, and CD45R0+ T lymphocyte subsets. Phenotyping of mononuclear cells that underwent transendothelial migration to MCP-2, MCP-3, RANTES, or MIP-1 alpha showed both monocytes and activated (CD26 high), memory-type (CD45R0+) T cells. Both CD4+ and CD8+ T lymphocytes were recruited, but not natural killer cells or significant numbers of B cells. MCP-2 was the only C-C chemokine tested that attracted a significant number of naive-type (CD45RA+) T lymphocytes. In the absence of endothelium, IL-8 but not IP-10 promoted modest but significant chemotoxis of CD3+ T lymphocytes. Our data support the hypothesis that C-C, not the C-X-C chemokines IL-8 or IP-10, promote transendothelial chemotaxis of T lymphocytes.
Roth
S J
SJ
Center for Blood Research, Boston, MA 02115, USA.
Carr
M W
MW
Springer
T A
TA
D017252:CD3 Complex / Q000502:physiology; D015496:CD4-Positive T-Lymphocytes / Q000502:physiology; D018414:CD8-Positive T-Lymphocytes / Q000502:physiology; D054410:Chemokine CCL7; D054412:Chemokine CCL8; D054357:Chemokine CXCL10; D018925:Chemokines / Q000737:chemistry / Q000494:pharmacology*; D019743:Chemokines, CXC; D002634:Chemotaxis, Leukocyte / Q000187:drug effects* / Q000276:immunology; D016207:Cytokines / Q000096:biosynthesis / Q000737:chemistry / Q000494:pharmacology*; D004730:Endothelium, Vascular / Q000276:immunology*; D006801:Humans; D016130:Immunophenotyping; D007371:Interferon-gamma / Q000494:pharmacology*; D016209:Interleukin-8 / Q000737:chemistry / Q000494:pharmacology*; D017493:Leukocyte Common Antigens / Q000502:physiology; D008567:Membranes, Artificial; D018945:Monocyte Chemoattractant Proteins / Q000494:pharmacology; D016176:T-Lymphocyte Subsets / Q000145:classification / Q000502:physiology*
C515450:CCL7 protein, human; C515453:CCL8 protein, human; D017252:CD3 Complex; D054410:Chemokine CCL7; D054412:Chemokine CCL8; D054357:Chemokine CXCL10; D018925:Chemokines; D019743:Chemokines, CXC; D016207:Cytokines; D016209:Interleukin-8; D008567:Membranes, Artificial; D018945:Monocyte Chemoattractant Proteins; D007371:Interferon-gamma; D017493:Leukocyte Common Antigens