Photothermal effects on murine mammary tumors using indocyanine green and an 808-nm diode laser: an in vivo efficacy study.
Murine mammary tumors were treated using indocyanine green and an 808 nm diode laser, and the in vivo chromophore-enhanced photothermal effects on the tumor burden and on tumor rat survival were investigated. The power of the laser was selected in the range of 5-10 W, and irradiation duration 3-5 min. One percent aqueous indocyanine green solution in a volume of 100-200 microliters was administered in situ, either acutely or 24 h prior to the treatment. The photothermal interaction was apparent under all our treatment conditions with a well-defined spatial containment in this study and the tumor growth was slowed after treatment. The post-treatment observation showed tumor recurrence and metastasis; no long-term survival was achieved with the single application of laser in conjunction with indocyanine green. Our results pose a question on the efficacy of the photothermal interaction even though tumor cell destruction can be achieved in a large and controlled scale. However, this highly selective photothermal impact on the tumor tissue did suggest that this method be applied repeatedly to be more effective and be used as the precursor of other modalities, such as chemotherapy, radiation therapy, immunotherapy, and surgery.
Chen
W R
WR
Oklahoma School of Science and Mathematics, Oklahoma City 73104, USA.
Adams
R L
RL
Higgins
A K
AK
Bartels
K E
KE
Nordquist
R E
RE
D000818:Animals; D004396:Coloring Agents / Q000494:pharmacology; D003131:Combined Modality Therapy; D005260:Female; D006979:Hyperthermia, Induced; D007208:Indocyanine Green / Q000494:pharmacology*; D007834:Lasers; D008325:Mammary Neoplasms, Experimental / Q000188:drug therapy / Q000628:therapy*; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000494:pharmacology*; D051381:Rats; D011920:Rats, Inbred WF; D013997:Time Factors
D004396:Coloring Agents; D017319:Photosensitizing Agents; D007208:Indocyanine Green