Phospholipid solubilization during detergent extraction of rhodopsin from photoreceptor disk membranes.
The solubilization of rhodopsin and phospholipids from disks prepared from bovine retinal rods was studied using five different detergents. The relative amounts of rhodopsin and lipid extracted during membrane solubilization differed dramatically with the nature of the surfactant; the two nonpolar detergents, Emulphogene (polyoxyethylene-10 tridecylether) and octylglucoside, removed more protein than lipid; two bile salt-related detergents, 3-[(3-cholamidopropyl)dimethylammonio]-1-propane sulfonate (Chaps) and taurocholate, released relatively more lipid than protein; and digitonin, which shares characteristics with both groups of detergents, extracted more lipid per mole of rhodopsin than the former two but less than the latter two. Solubilization was temperature-dependent with all five detergents, though particularly so with octylglucoside: concentrations adequate for the total micellation of disks at 23 degrees C were ineffectual at 4 degrees C. In total solubilizates of disks, the amount of lipid recovered in rhodopsin-lipid-detergent micelles showed a closer correlation with the critical micellar concentration (CMC) than with the chemical nature of the detergent (octylglucoside > taurocholate > Chaps > digitonin > Emulphogene). The higher the CMC, the larger the amount of lipid associated to the solubilized rhodopsin and the larger the amount of lipid reassociated to rhodopsin upon surfactant dilution. For all five detergents, the lipid progressively extracted from disks during solubilization was relatively richer in phosphatidylcholine (PC) than the lipid in the original membranes. The lipid which tended to be associated with rhodopsin in protein-lipid-detergent mixed micelles was also consistently richer in PC than that present in lipid-detergent micelles. Bleaching of solubilized rhodopsin decreased the amount of lipid in protein-lipid-detergent micelles. Rhodopsin photolytic transitions were faster in nonionic than in bile salt-related detergents.
Aveldaño
M I
MI
Instituto de Investigaciones Bioquímicas, Universidad Nacional del Sur and Consejo Nacional de Investigaciones Científicas y Técnicas, Bahía Blanca, Argentina.
D000818:Animals; D002417:Cattle; D002793:Cholic Acids / Q000494:pharmacology; D003902:Detergents / Q000494:pharmacology*; D004072:Digitonin / Q000494:pharmacology; D005960:Glucosides / Q000494:pharmacology; D008027:Light; D008566:Membranes / Q000187:drug effects; D008823:Micelles; D008956:Models, Chemical; D010743:Phospholipids / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000494:pharmacology; D012243:Rhodopsin / Q000302:isolation & purification* / Q000528:radiation effects; D012374:Rod Cell Outer Segment / Q000187:drug effects*; D012995:Solubility / Q000187:drug effects; D013053:Spectrophotometry; D013656:Taurocholic Acid / Q000494:pharmacology; D013696:Temperature
D002793:Cholic Acids; D003902:Detergents; D005960:Glucosides; D008823:Micelles; D010743:Phospholipids; C007590:emulphogene BC 720; C018619:octyl-beta-D-glucoside; D011092:Polyethylene Glycols; D013656:Taurocholic Acid; D012243:Rhodopsin; D004072:Digitonin; C028213:3-((3-cholamidopropyl)dimethylammonium)-1-propanesulfonate