title: Interleukin 10 release during endotoxaemia in chimpanzees: role of platelet-activating factor and interleukin 6.
abstract: Interleukin (IL-)10 has been demonstrated to inhibit endotoxin-induced production of a number of pro-inflammatory cytokines. The present study sought to compare the appearances in the circulation of IL-10, IL-6 and IL-8, and to assess the roles of endogenously produced platelet-activating factor (PAF) and IL-6 in IL-10 release during endotoxaemia in chimpanzees. Intravenous injection of endotoxin (lot EC-5, 4 ng/kg, n = 8) induced a transient rise in serum IL-10 concentrations, peaking after 2 h (213 +/- 70 pg/ml; P < 0.05). No correlations existed between peak IL-10 levels, and peak IL-6 and IL-8 levels. Neither infusion of the specific PAF antagonist TCV-309 (n = 4), nor infusion of a neutralizing anti-IL-6 monoclonal antibody (n = 4) influenced endotoxin-induced IL-10 release. IL-10 release elicited by injection of endotoxin is not mediated by PAF or IL6.
authors.affiliation: Department of Internal Medicine, Academic Medical Center, University of Amsterdam, The Netherlands.
authors.forename: T
authors.initials: T
authors.lastname: van der Poll
authors.forename: J
authors.initials: J
authors.lastname: Jansen
authors.forename: M
authors.initials: M
authors.lastname: Levi
authors.forename: H
authors.initials: H
authors.lastname: ten Cate
authors.forename: C E
authors.initials: CE
authors.lastname: Hack
authors.forename: L A
authors.initials: LA
authors.lastname: Aarden
authors.forename: J W
authors.initials: JW
authors.lastname: ten Cate
authors.forename: S J
authors.initials: SJ
authors.lastname: van Deventer
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage; D004731:Endotoxins; D004926:Escherichia coli; D007275:Injections, Intravenous; D016753:Interleukin-10 / Q000096:biosynthesis*; D015850:Interleukin-6 / Q000096:biosynthesis / Q000502:physiology*; D016209:Interleukin-8 / Q000096:biosynthesis; D007546:Isoquinolines / Q000494:pharmacology; D002679:Pan troglodytes; D010972:Platelet Activating Factor / Q000502:physiology*; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology; D011726:Pyridinium Compounds / Q000494:pharmacology; D044005:Tetrahydroisoquinolines; D014115:Toxemia / Q000139:chemically induced / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D004731:Endotoxins; D015850:Interleukin-6; D016209:Interleukin-8; D007546:Isoquinolines; D010972:Platelet Activating Factor; D010975:Platelet Aggregation Inhibitors; D011726:Pyridinium Compounds; D044005:Tetrahydroisoquinolines; D016753:Interleukin-10; C067430:TCV 309