Purification of chlorpromazine-sensitive GTPase from rat cerebral cortex.
The chlorpromazine-sensitive GTPase from the cell membrane of rat cerebral cortex was purified to homogenity by using DEAE Bio-Gel A agarose, hydroxyapatite and heparin agarose chromatography. The purified chlorpromazine-sensitive GTPase was purified 370-fold to obtain a final specific activity of 40 mumol GTP hydrolyzed2min/mg protein. The purified enzyme was inhibited by chlorpromazine but not by compound 48/80. Magnesium was required for its activity instead of calcium. The purified enzyme had an apparent pH optimum of 8.0, and molecular weight was estimated to be 58,000.
Chen
L C
LC
Department of Pharmacology, National Yang-Ming University, Taipei, Taiwan, Republic of China.
Wu
C Y
CY
Chen
C F
CF
Chiang
C F
CF
D000818:Animals; D002462:Cell Membrane / Q000201:enzymology; D002540:Cerebral Cortex / Q000201:enzymology*; D002746:Chlorpromazine / Q000494:pharmacology*; D019826:Cholic Acid; D002793:Cholic Acids; D002845:Chromatography / Q000379:methods; D017886:Durapatite; D004791:Enzyme Inhibitors / Q000494:pharmacology; D020558:GTP Phosphohydrolases / Q000037:antagonists & inhibitors / Q000302:isolation & purification* / Q000378:metabolism; D006493:Heparin; D006863:Hydrogen-Ion Concentration; D008274:Magnesium / Q000494:pharmacology; D008970:Molecular Weight; D051381:Rats; D017207:Rats, Sprague-Dawley; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology
D002793:Cholic Acids; D004791:Enzyme Inhibitors; D003189:p-Methoxy-N-methylphenethylamine; D006493:Heparin; D017886:Durapatite; D020558:GTP Phosphohydrolases; D019826:Cholic Acid; D008274:Magnesium; D002746:Chlorpromazine