title: The guanylyl cyclase core of an atrial natriuretic peptide receptor: enzymatic properties and basis for cooperativity.
abstract: The enzymatic properties of a cloned atrial natriuretic peptide receptor are described. The renatured catalytic core had maximal activity with Mn2+, and all nucleoside triphosphates inhibited the enzyme competitively. The catalytic specificity of the enzyme was tested directly. The cyclase reaction was specific for guanine, producing cGMP and cyclic deoxyGMP. Surprisingly, deoxyguanylyl cyclase kinetics were classical, unlike the positive cooperativity seen for guanylyl cyclase activity, suggesting that the 2' hydroxyl group of GTP is necessary for the allosteric mechanism.
authors.affiliation: Department of Discovery Biology, Selectide Corporation, Tucson, AZ 85737, USA.
authors.forename: D S
authors.initials: DS
authors.lastname: Thorpe
authors.forename: S
authors.initials: S
authors.lastname: Niu
authors.forename: E
authors.initials: E
authors.lastname: Morkin
mesh_terms: D000494:Allosteric Regulation; D002413:Cations, Divalent; D003854:Deoxyribonucleotides / Q000737:chemistry / Q000378:metabolism; D006160:Guanosine Triphosphate / Q000378:metabolism; D006162:Guanylate Cyclase / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000378:metabolism; D007700:Kinetics; D009711:Nucleotides / Q000737:chemistry / Q000378:metabolism; D017461:Receptors, Atrial Natriuretic Factor / Q000737:chemistry*; D011994:Recombinant Proteins; D015398:Signal Transduction; D013379:Substrate Specificity
chemical_list: D002413:Cations, Divalent; D003854:Deoxyribonucleotides; D009711:Nucleotides; D011994:Recombinant Proteins; D006160:Guanosine Triphosphate; D006162:Guanylate Cyclase; D017461:Receptors, Atrial Natriuretic Factor