SINE and LINE within human centromeres.
A number of the Alu and L1 elements present within the centromeric regions of the human chromosomes have been analyzed by polymerase chain reaction amplification. The oligonucleotide primers were homologous to the 3' end consensus sequences of either Alu or L1 in conjunction with an oligonucleotide primer homologous to alphoid sequences specific to different chromosomes. This allowed one to detect an unusual number of Alu and L1 polymorphisms at different loci. It is proposed that this results from molecular rearrangements which occur within the alpha-satellite DNA in which they are embedded (Marçais et al. J. Mol. Evol. 33:42-48, 1991) and not because the centromeric regions are targets for new insertions of such elements. The same analyses were made on cosmids and YACs originating from the centromeric region of chromosome 21 as well as on a collection of somatic hybrids containing chromosome 21 centromere as unique common human genetic material. The results were consistent with the above hypothesis.
Prades
C
C
UPR 9008 Cnrs, Institut de biologie, Montpellier, France.
Laurent
A M
AM
Puechberty
J
J
Yurov
Y
Y
Roizés
G
G
D002503:Centromere / Q000235:genetics*; D002874:Chromosome Mapping; D002877:Chromosomes, Human / Q000235:genetics; D004251:DNA Transposable Elements / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics*; D005819:Genetic Markers; D006801:Humans; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*
D004251:DNA Transposable Elements; D004268:DNA-Binding Proteins; D005819:Genetic Markers; C077214:L1Hs-encoded protein p40, human