title: Interaction of echicetin with a high affinity thrombin binding site on platelet glycoprotein GPIb.
abstract: Echicetin, a protein isolated from Echis carinatus snake venom, inhibited platelet aggregation and secretion induced by low concentrations of thrombin ( < 0.2 U/ml), by binding to platelet glycoprotein Ib (GPIb). The inhibition was not observed when the platelets were stimulated with higher concentrations of thrombin ( > 0.2 U/ml). Echicetin competed with thrombin for binding to the high affinity site on GPIb. Thrombin also inhibited 50% of the binding of 125I-echicetin to the platelets.
authors.affiliation: Sol Sherry Thrombosis Research Center, Temple University School of Medicine, Philadelphia, PA 19140, USA.
authors.forename: M
authors.initials: M
authors.lastname: Peng
authors.forename: F A
authors.initials: FA
authors.lastname: Emig
authors.forename: A
authors.initials: A
authors.lastname: Mao
authors.forename: W
authors.initials: W
authors.lastname: Lu
authors.forename: E P
authors.initials: EP
authors.lastname: Kirby
authors.forename: S
authors.initials: S
authors.lastname: Niewiarowski
authors.forename: M A
authors.initials: MA
authors.lastname: Kowalska
mesh_terms: D000595:Amino Acid Sequence; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism; D002352:Carrier Proteins; D000939:Epitopes; D006801:Humans; D008969:Molecular Sequence Data; D010455:Peptides / Q000097:blood; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*; D019038:Platelet Glycoprotein GPIb-IX Complex / Q000378:metabolism*; D010980:Platelet Membrane Glycoproteins / Q000378:metabolism; D011506:Proteins / Q000276:immunology / Q000378:metabolism / Q000494:pharmacology*; D011956:Receptors, Cell Surface / Q000378:metabolism; D018179:Receptors, Thrombin / Q000378:metabolism*; D012636:Secretory Rate / Q000187:drug effects; D013917:Thrombin / Q000494:pharmacology; D014757:Viper Venoms / Q000097:blood / Q000494:pharmacology*
chemical_list: D002352:Carrier Proteins; D000939:Epitopes; D010455:Peptides; D010975:Platelet Aggregation Inhibitors; D019038:Platelet Glycoprotein GPIb-IX Complex; D010980:Platelet Membrane Glycoproteins; D011506:Proteins; D011956:Receptors, Cell Surface; D018179:Receptors, Thrombin; D014757:Viper Venoms; C028254:von Willebrand factor receptor; C080995:echicetin; D013917:Thrombin