Differential effects of endogenous and exogenous nitric oxide on the release of endothelin-1 from the intact perfused rat adrenal gland in situ.
Studies using an inhibitor of nitric oxide (NO) synthesis have suggested that endogenous NO may have a role in regulating endothelin release. We investigated the effect of endogenous and exogenous nitric oxide (NO) on the release of irET-1. L-NAME stimulated, but L-arginine inhibited irET-1 release. Perfusing sodium nitroprusside (SNP), however, did not inhibit irET-1 secretion. CyclicGMP, the second messenger for NO action, was stimulated by SNP but not by L-arginine. These data demonstrate that endogenous NO inhibits of irET-1, in a manner which is independent of cGMP, and suggest that this action may contribute to the vasodilatory effect of NO.
Hinson
J P
JP
Department of Biochemistry, Faculty of Basic Medical Sciences, Queen Mary and Westfield College, London, UK. J.Hinson@qmw.ac.uk
Kapas
S
S
Cameron
L A
LA
D000311:Adrenal Glands / Q000187:drug effects* / Q000378:metabolism* / Q000502:physiology; D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives / Q000494:pharmacology; D006152:Cyclic GMP / Q000096:biosynthesis; D016232:Endothelins / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D066298:In Vitro Techniques; D008297:Male; D019331:NG-Nitroarginine Methyl Ester; D009569:Nitric Oxide / Q000494:pharmacology* / Q000502:physiology*; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors; D009599:Nitroprusside / Q000494:pharmacology; D010477:Perfusion; D051381:Rats; D017208:Rats, Wistar; D015290:Second Messenger Systems / Q000187:drug effects / Q000502:physiology; D014664:Vasodilation / Q000502:physiology
D016232:Endothelins; D004791:Enzyme Inhibitors; D009599:Nitroprusside; D009569:Nitric Oxide; D001120:Arginine; D019001:Nitric Oxide Synthase; D006152:Cyclic GMP; D019331:NG-Nitroarginine Methyl Ester