Generation of primary tumor-specific CTL in vitro to immunogenic and poorly immunogenic mouse tumors.
This study investigated the generation of primary tumor-specific CTL activity in vitro to several mouse tumors. We report that the development of optimal primary tumor-specific CTL to the P815 mastocytoma, the EL4 thymoma, and the Lewis lung carcinoma is dependent on tumor Ags, on enhancement of T cell costimulation by B7.1, and on exogenous T helper activity in the form of IL-2 and IL-4. A relatively low concentration of IL-2 and IL-4 was required to limit the induction of lymphokine-activated killer cells. In the case of P815, the CTL were directed toward molecularly defined tumor rejection Ags. These primary cultures yielded long term T cell lines that were heterogeneous in fine tumor Ag specificity and in cytokine production.
Liu
B
B
Department of Microbiology and Immunology, University of Miami School of Medicine, FL 33136, USA.
Podack
E R
ER
Allison
J P
JP
Malek
T R
TR
D000818:Animals; D000951:Antigens, Neoplasm / Q000494:pharmacology; D018122:B7-1 Antigen / Q000494:pharmacology; D018827:Carcinoma, Lewis Lung / Q000378:metabolism; D003602:Cytotoxicity, Immunologic; D006131:Growth Inhibitors / Q000378:metabolism; D016130:Immunophenotyping; D007376:Interleukin-2 / Q000276:immunology; D008213:Lymphocyte Activation; D012515:Mast-Cell Sarcoma / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology* / Q000637:transplantation; D014407:Tumor Cells, Cultured
D000951:Antigens, Neoplasm; D018122:B7-1 Antigen; D006131:Growth Inhibitors; D007376:Interleukin-2