title: Stability of porcine reproductive and respiratory syndrome virus in the presence of fomites commonly found on farms.
abstract: OBJECTIVE
  To determine the survival of porcine reproductive and respiratory syndrome virus (PRRSV) on nonliving substances (fomites) at 25 to 27 C.
  
  
  METHODS
  Prospective controlled study.
  
  
  METHODS
  3 solid, 6 porous, and 7 liquid fomites.
  
  
  METHODS
  The fomites were contaminated with known concentrations of PRRSV. Samples for virus isolation were obtained on day 0 through day 11, assayed in cell cultures, and stained with fluorescent antibody conjugate.
  
  
  RESULTS
  The virus was recovered only on day-0 samples of alfalfa, wood shavings, straw, plastic, boot rubber, and stainless steel. Virus was isolated from city water through day 11, from well water through day 9, and from 2 buffer solutions for 4 and 6 days. The virus was isolated only on day 0 from swine saliva, urine, and fecal slurry.
  
  
  CONCLUSIONS
  Results indicated that PRRSV is a fairly labile virus, but because of its duration of viability in water, contamination of drinking water and lagoons by PRRSV-shedding swine would serve as sources of virus to infect susceptible swine.
authors.affiliation: Department of Microbiology, Immunology, and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Ames 50011, USA.
authors.forename: E C
authors.initials: EC
authors.lastname: Pirtle
authors.forename: G W
authors.initials: GW
authors.lastname: Beran
mesh_terms: D000821:Animal Feed / Q000821:virology; D000818:Animals; D018018:Arterivirus / Q000302:isolation & purification / Q000502:physiology*; D018174:Arterivirus Infections / Q000662:veterinary / Q000821:virology; D004783:Environmental Microbiology; D005243:Feces / Q000821:virology; D000455:Medicago sativa / Q000821:virology; D010969:Plastics; D006109:Poaceae / Q000821:virology; D012408:Rubber; D012463:Saliva / Q000821:virology; D013193:Stainless Steel; D013552:Swine; D013553:Swine Diseases / Q000821:virology; D014556:Urine / Q000821:virology; D014871:Water Microbiology; D014881:Water Supply; D014934:Wood; D003313:Zea mays / Q000821:virology
chemical_list: D010969:Plastics; D013193:Stainless Steel; D012408:Rubber