title: Automatic-interactive measurement of resorption cavities in transiliac bone biopsies and correlation with deoxypyridinoline.
abstract: Measuring bone resorption accurately by histomorphometry of bone biopsies is a challenge. Several techniques have been proposed including the measurement of eroded surfaces and resorption depth, but they have not been compared between themselves nor with biochemical assessment of bone resorption. In addition, there is a need for a rapid method that could be used more routinely. We describe here an automatic interactive method using a color analyzer (Visiolab, BIOCOM, France) with a specific software for the evaluation of erosion depth, eroded volume, eroded surface, osteoclast number, and surface. Thirty transiliac undecalcified bone biopsies stained with Goldner's trichrome were used in this study, taken from subjects suffering from osteoporosis or primary hyperparathyroidism. At the time of the biopsy a 2 h fasting morning urine sample was collected for measurement by HPLC of total deoxypyridinoline, the most sensitive marker of bone resorption. There was a highly significant correlation between maximum erosion depth measured directly and the one calculated according to the count of eroded lamellae (E. F. Eriksen, et al. Metab Bone Dis Relat Res 5:243-252; 1984) (r = 0.76; p = 0.0001). A significant correlation was found between urinary deoxypyridinoline and eroded volume/bone volume in cancellous and endocortical bone measured with the automatic interactive technique (r = 0.48; p = 0.007). In contrast, other histological indexes of bone resorption did not correlate with urinary deoxypyridinoline. The volume of resorption cavities appears to be the most valid index of bone resorption rate as it was correlated with the urinary excretion of total deoxypyridinoline.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: INSERM Research Unit 403, Lyon, France.
authors.forename: J P
authors.initials: JP
authors.lastname: Roux
authors.forename: M E
authors.initials: ME
authors.lastname: Arlot
authors.forename: E
authors.initials: E
authors.lastname: Gineyts
authors.forename: P J
authors.initials: PJ
authors.lastname: Meunier
authors.forename: P D
authors.initials: PD
authors.lastname: Delmas
mesh_terms: D000328:Adult; D000596:Amino Acids / Q000652:urine*; D001706:Biopsy; D001862:Bone Resorption / Q000473:pathology* / Q000652:urine; D002851:Chromatography, High Pressure Liquid; D003124:Colorimetry; D005260:Female; D006801:Humans; D006961:Hyperparathyroidism / Q000473:pathology / Q000652:urine; D007085:Ilium / Q000378:metabolism / Q000473:pathology*; D008297:Male; D010010:Osteoclasts / Q000473:pathology* / Q000648:ultrastructure; D010024:Osteoporosis / Q000473:pathology / Q000652:urine
chemical_list: D000596:Amino Acids; C036020:deoxypyridinoline