title: Differential scanning calorimetric studies of superficial digital flexor tendon degeneration in the horse.
abstract: Differential scanning calorimetry (DSC) of equine superficial digital flexor tendons revealed the presence of a small exothermic peak at 23 degrees C of unknown origin, and a large endothermic peak at 70 degrees C due to denaturation of cross-linked collagen fibres. In the central degenerated core of damaged tendons the denaturation temperature remained at 70 degrees C but the enthalpy decreased in relation to the extent of degeneration of the tendon. We suggest that this reduction in enthalpy is due to depolymerisation and denaturation of the collagen fibres. This contention is supported by the observed increased activity of the degradative enzyme cathepsin B secreted by the fibroblasts. DSC analysis of cultured porcine tendon fibroblasts revealed a multicomponent endotherm, denaturation beginning at 46 degrees C, a temperature capable of being achieved within the tendon during intensive exercise. DSC clearly has considerable potential in complementing morphological and biochemical studies to determine the aetiology and progress of equine tendon degeneration.
authors.affiliation: Department of Veterinary Medicine, School of Veterinary Science, University of Bristol, Langford, UK.
authors.forename: C A
authors.initials: CA
authors.lastname: Miles
authors.forename: R J
authors.initials: RJ
authors.lastname: Wardale
authors.forename: H L
authors.initials: HL
authors.lastname: Birch
authors.forename: A J
authors.initials: AJ
authors.lastname: Bailey
mesh_terms: D000818:Animals; D002152:Calorimetry, Differential Scanning / Q000379:methods / Q000662:veterinary; D002401:Cathepsin B / Q000032:analysis / Q000378:metabolism; D002460:Cell Line; D003094:Collagen / Q000032:analysis / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism / Q000473:pathology / Q000502:physiology; D006734:Horse Diseases / Q000378:metabolism / Q000473:pathology*; D006736:Horses / Q000293:injuries* / Q000378:metabolism; D013696:Temperature; D013708:Tendon Injuries / Q000378:metabolism / Q000473:pathology / Q000662:veterinary*; D013710:Tendons / Q000737:chemistry / Q000378:metabolism* / Q000473:pathology*; D014867:Water / Q000032:analysis
chemical_list: D014867:Water; D003094:Collagen; D002401:Cathepsin B