title: Anticoagulant poisoning in animals: a simple new high-performance thin-layer chromatographic (HPTLC) method for the simultaneous determination of eight anticoagulant rodenticides in liver samples.
abstract: The purpose of this study was to develop and evaluate a technique for the analysis of anticoagulant rodenticides in serum and liver samples using a new high-performance thin-layer chromatographic apparatus. Detection limits were estimated at 0.2 micrograms/g in liver extracts for eight different substances. Overall, this technique was repeatable and reproducible. The percent recovery was greater than 87% for each substance. Liver and serum samples of animals known to be exposed to one anticoagulant and showing clinical signs of poisoning were analyzed. Concentrations measured varied between 0.2 and 3 micrograms/g (liver extracts). Only blood samples from one dog could be analyzed. The concentration was 150 ng/mL the first day after admission and 140 ng/mL the following day. Analyses are technically easily and rapidly performed, and they are inexpensive. Therefore, this technique could be a valuable alternative to current high-performance liquid chromatographic methods.
authors.affiliation: Département des Sciences Pharmaceutiques et Toxicologiques, Ecole Nationale Vétérinaire de Lyon, Marcy l'Etoile, France.
authors.forename: P J
authors.initials: PJ
authors.lastname: Berny
authors.forename: T
authors.initials: T
authors.lastname: Buronfosse
authors.forename: G
authors.initials: G
authors.lastname: Lorgue
mesh_terms: D015110:4-Hydroxycoumarins / Q000032:analysis / Q000506:poisoning; D000818:Animals; D000925:Anticoagulants / Q000032:analysis* / Q000506:poisoning; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer / Q000379:methods*; D004285:Dogs; D007189:Indans / Q000032:analysis / Q000506:poisoning; D008099:Liver / Q000737:chemistry*; D015203:Reproducibility of Results; D012378:Rodenticides / Q000032:analysis* / Q000506:poisoning; D014859:Warfarin / Q000032:analysis / Q000506:poisoning
chemical_list: D015110:4-Hydroxycoumarins; D000925:Anticoagulants; D007189:Indans; D012378:Rodenticides; C004662:chlorophacinone; D014859:Warfarin; C013418:bromfenacoum; C021636:bromadiolone; C060340:difethialone; C004699:coumatetralyl; C012903:difenacoum