Cloning of the cDNA for a novel photoreceptor protein.
A subtractive cDNA cloning strategy was used to isolate a 1381-base pair human retina-specific cDNA, human retinal gene 4 (HRG4), which hybridized to a 1.4-kilobase message in the retina and encoded a 240-amino acid acidic protein with a calculated molecular mass of 26,964 Da. The proximal 1/4 of the conceptual protein sequence was rich in glycine (18%) and proline (20%), had a predicted secondary structure of turns, and showed a loose similarity (19-24%) to various alpha-collagen sequences, while the distal 2/4 consisted of a mixture of alpha-helices, beta-sheets, and turns. Genomic Southern analysis with HRG4 showed cross-hybridizing sequences in six different species, and HRG4 was 92% homologous with a 1264-base pair rat cDNA (rat retinal gene 4; RRG4) at the protein level. The region of 100% identity between the two sequences corresponded to the distal 3/4 of the protein sequence consisting of mixed secondary structures, suggesting a functionally important domain. In vitro transcription and translation corroborated the open reading frames corresponding to HRG4 and RRG4 in the cDNAs. Expression of HRG4 in the retina was localized to the photoreceptors by in situ hybridization. Developmentally, RRG4 began to be highly expressed around postnatal day 5 in the rat outer retina when the photoreceptors begin to differentiate and rapidly increased in expression to reach the mature adult level by postnatal day 23. No diurnal fluctuation in expression of RRG4 was seen.
Higashide
T
T
Bascom Palmer Eye Institute, University of Miami School of Medicine, Florida 33136, USA.
Murakami
A
A
McLaren
M J
MJ
Inana
G
G
D048868:Adaptor Proteins, Signal Transducing; D000328:Adult; D000375:Aging / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001482:Base Composition; D001483:Base Sequence; D001921:Brain / Q000378:metabolism; D003001:Cloning, Molecular; D018076:DNA, Complementary; D005136:Eye Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D005333:Fetus; D006801:Humans; D017403:In Situ Hybridization; D007223:Infant; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010786:Photoreceptor Cells / Q000378:metabolism*; D014176:Protein Biosynthesis; D017433:Protein Structure, Secondary; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D051381:Rats; D012160:Retina / Q000254:growth & development / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic
D048868:Adaptor Proteins, Signal Transducing; D018076:DNA, Complementary; D005136:Eye Proteins; D012333:RNA, Messenger; C098032:UNC119 protein, human