Early elemental and ionic changes in cultured cells after stimulation with epidermal growth factor.
Stimulation of A431 cells (a human vulval epidermal cell line) with 50 ng/ml of epidermal growth factor (EGF) in the presence of 1.7 mM extracellular calcium produced a sharp and sustained rise in intracellular ionic Ca2+, increased elemental Na, decreased K and a rise in Ca. In the absence of extracellular calcium, the initial Ca2+ rise remained but the sustained elevation of intracellular Ca2+ was abolished, Na and K fluxes were variable and the Ca did not change. Increased Na and decreased K was marked at 2 minutes and returned to the control value after 60 minutes. The increase in Ca was an early event. Cells stimulated with EGF showed a pronounced morphological disruption, especially the mitochondria. The response of NR6/SA3 and NR6/DC7 cells (genetically engineered rodent fibroblast cell lines) to EGF stimulation was higher than that of the A431 cells, as was the resting cytoplasmic Ca2+. Untreated NR6/SA3 and NR6/DC7 cells possessed an increased Na/K ratio when compared with A431 cells.
Beesley
J E
JE
Wellcome Research Laboratories, Beckenham, Kent, U.K.
Hall
S R
SR
Page
M J
MJ
Affleck
K
K
D000818:Animals; D002118:Calcium / Q000378:metabolism* / Q000494:pharmacology; D002460:Cell Line; D003592:Cytophotometry; D004577:Electron Probe Microanalysis; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D004817:Epidermis / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D005260:Female; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D006801:Humans; D007473:Ion Channels / Q000378:metabolism*; D008855:Microscopy, Electron, Scanning; D008928:Mitochondria / Q000187:drug effects / Q000648:ultrastructure; D011188:Potassium / Q000378:metabolism*; D012964:Sodium / Q000378:metabolism*; D014844:Vulva / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure
D007473:Ion Channels; D004815:Epidermal Growth Factor; D012964:Sodium; D011188:Potassium; D002118:Calcium