Ovarian production of IL6 and its potential inhibitory effect on progesterone secretion in Cynomolgus fascicularis.
We explored the potential relevance of interleukin-6 (IL6) to ovarian function in cynomolgus monkey females which were treated to induce multiple follicular growth. Present work gave evidences that IL6 was produced in the ovulatory follicle after hCG administration by using anti-IL6 monoclonal antibody and immunohistochemical techniques. IL6 was produced by granulosa cells in cultures. However, this production was not stimulated by adding to cultures FSH or Bt2cAMP, known to induce IL6 production in various cell types. IL6 was shown to antagonize luteinizing FSH effects. Inhibitory IL6 effect was probably mediated at a site distal to cAMP generation since it had no effect on progesterone production induced by dibutyryl cAMP treatment. The presence of IL6 in the ovulatory follicle show that inflammatory cytokines may play a role at ovulation. On the other hand, its inhibitory effect on progesterone production by granulosa cells could not likely affect the process of luteinization which is controlled by LH/hCG.
Machelon
V
V
INSERM U. 355, Institut fédératif sur les cytokines de Paris-Sud, Clamart, France.
Gougeon
A
A
Duquenne
C
C
Testart
J
J
Wallon
C
C
Delattre
R M
RM
Emilie
D
D
Galanaud
P
P
D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D002478:Cells, Cultured; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D006107:Granulosa Cells / Q000378:metabolism*; D015850:Interleukin-6 / Q000378:metabolism* / Q000494:pharmacology*; D008252:Macaca fascicularis; D011374:Progesterone / Q000037:antagonists & inhibitors / Q000378:metabolism*
D015850:Interleukin-6; D011374:Progesterone; D003994:Bucladesine; D005640:Follicle Stimulating Hormone