title: The measurement of swimming velocity of Vibrio cholerae and Pseudomonas aeruginosa using the video tracking methods.
abstract: The swimming velocities of two monotrichous flagellated bacteria were measured by a computer-assisted video tracking method. Tracing the moving path of the individual bacterium revealed that the bacterial cell did not swim continuously in a straight direction, but frequently changed swimming direction and velocity. The average swimming velocities calculated from the 3-sec path were 75.4 +/- 9.4 microns/sec in four strains of Vibrio cholerae and 51.3 +/- 8.4 microns/sec in five strains of Pseudomonas aeruginosa. These results suggest that V. cholerae swim faster than P. aeruginosa at 30 C in nutrient broth. This method is useful for a detailed analysis of bacterial movement and moving patterns in different environmental conditions.
authors.affiliation: Department of Bacteriology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Shigematsu
authors.forename: Y
authors.initials: Y
authors.lastname: Meno
authors.forename: H
authors.initials: H
authors.lastname: Misumi
authors.forename: K
authors.initials: K
authors.lastname: Amako
mesh_terms: D009068:Movement; D011550:Pseudomonas aeruginosa / Q000502:physiology*; D014734:Vibrio cholerae / Q000502:physiology*; D014743:Videotape Recording