Gamma-radiation induces upregulation of Bax protein and apoptosis in radiosensitive cells in vivo.
Lymphoid cells and small intestinal epithelial (SIE) cells are among the most radiosensitive in the body. The factors that account for the differential sensitivity to gamma-radiation among different tissue-types remain poorly understood, but can only partly be explained by differences in rates of cell proliferation. Here we demonstrate that exposure of mice to 800 cGy of gamma-radiation results in rapid elevations in the levels of the Bax protein, a pro-apoptotic member of the Bcl-2 protein family, in lymphoid cells and SIEs. gamma-Radiation-induced increase in Bax protein were evident within 2 h and persisted for at least 24 h, as determined by immunoblotting and immunohistochemical assays. Increases in Bax were followed by massive apoptosis in lymphoid organs and in the small intestinal crypts, as determined by morphological criteria and in situ end-labeling of fragmented nuclear DNA by terminal deoxynucleotidyl transferase (TUNEL method). Radiation did not induce elevations in Bax or apoptosis in radioresistant tissues such as heart, skeletal muscle, brain, kidney, liver, lung, vascular smooth muscle and connective tissue. The findings suggest that Bax may be one of the mediators of radiation-induced apoptosis in vivo.
Kitada
S
S
La Jolla Cancer Research Foundation, California 92037, USA.
Krajewski
S
S
Miyashita
T
T
Krajewska
M
M
Reed
J C
JC
D000818:Animals; D017209:Apoptosis / Q000528:radiation effects*; D005720:Gamma Rays; D051379:Mice; D009928:Organ Specificity; D011518:Proto-Oncogene Proteins / Q000032:analysis / Q000528:radiation effects*; D019253:Proto-Oncogene Proteins c-bcl-2; D011836:Radiation Tolerance; D016159:Tumor Suppressor Protein p53 / Q000502:physiology; D015854:Up-Regulation; D051028:bcl-2-Associated X Protein
C494827:Bax protein, mouse; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D016159:Tumor Suppressor Protein p53; D051028:bcl-2-Associated X Protein