Identification and localization of gene expression of a low M(r) GTP-binding protein, ram p25 in pituitary gland.
The localization of gene expression of a low M(r) GTP-binding protein, ram p25, was examined throughout the entire brain of adult rat by in situ hybridization. ram-mRNA was expressed predominantly in anterior and intermediate lobes of pituitary gland (hypophysis), and weakly in hippocampal formation. In other parts of brain, no significant expression of ram-mRNA was detected, indicating that ram p25 may have an important role(s) in adenohypophysis. In 15-day-fetal rat, the mRNA was observed to be expressed strongly in the primitive anterior lobe of pituitary gland. The ram p25 was also detected in bovine pituitary gland by a specific antibody and was purified to near homogeneity by stepwise column chromatographies. Biochemical characterization of the purified ram p25 revealed that the profile of [35S]GTP gamma S-binding activity was almost the same as that of recombinant ram p25 expressed in E. coli.
Nagata
K
K
Department of Molecular Pathobiochemistry, Gifu University School of Medicine, Japan.
Sakagami
H
H
Kondo
H
H
Nozawa
Y
Y
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D003600:Cytosol / Q000378:metabolism; D017931:DNA Primers / Q000737:chemistry; D019204:GTP-Binding Proteins / Q000235:genetics* / Q000378:metabolism*; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D010902:Pituitary Gland / Q000378:metabolism*; D051381:Rats; D020691:rab GTP-Binding Proteins; D000075745:rab27 GTP-Binding Proteins
D017931:DNA Primers; D000075745:rab27 GTP-Binding Proteins; D019204:GTP-Binding Proteins; C512961:Rab27a protein, rat; D020691:rab GTP-Binding Proteins