Interleukin-10 inhibits cytokine generation from mast cells.
This report examines the effects of recombinant murine interleukin-10 (rmIL-10) on antigen-induced beta-hexosaminidase, leukotriene (LT)C4 and cytokine release from mouse bone marrow-derived mast cells (BMMC). BMMC sensitized to hapten-monoclonal IgE directed against dinitrophenol-bovine serum albumin (DNP-BSA) and challenged with 10 ng/ml DNP-BSA generated beta-hexosaminidase and LTC4-like material which was followed by tumor necrosis factor-alpha (TNF-alpha) and granulocyte-macrophage colony-stimulating factor (GM-CSF) mRNA expression and protein release. Incubation of BMMC with 1-100 ng/ml rmIL-10 inhibited cytokine generation, without affecting beta-hexosaminidase and LTC4-like material release. TNF-alpha, but not GM-CSF mRNA expression, was also diminished in rmIL-10-treated BMMC, suggesting that down-regulation of cytokine production by rmIL-10 involves different mechanisms. These results identify a novel biological action of IL-10 as an inhibitor of cytokine production by stimulated mast cells.
Arock
M
M
Laboratoire d'Hématologie Cellulaire et Moléculaire, Faculté de Pharmacie, Paris, France.
Zuany-Amorim
C
C
Singer
M
M
Benhamou
M
M
Pretolani
M
M
D000818:Animals; D001854:Bone Marrow Cells; D000001:Calcimycin / Q000494:pharmacology; D002478:Cells, Cultured; D016207:Cytokines / Q000037:antagonists & inhibitors* / Q000096:biosynthesis* / Q000235:genetics; D004140:Dinitrophenols / Q000494:pharmacology; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000096:biosynthesis / Q000235:genetics; D006241:Haptens / Q000494:pharmacology; D016753:Interleukin-10 / Q000494:pharmacology*; D008407:Mast Cells / Q000187:drug effects* / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D011994:Recombinant Proteins / Q000494:pharmacology; D012710:Serum Albumin, Bovine / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis / Q000235:genetics
D016207:Cytokines; D004140:Dinitrophenols; D006241:Haptens; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; C034638:dinitrophenyl-bovine serum albumin; D016753:Interleukin-10; D012710:Serum Albumin, Bovine; D000001:Calcimycin; D016178:Granulocyte-Macrophage Colony-Stimulating Factor