Synergy of simultaneous administration of ofloxacin and granulocyte colony-stimulating factor in killing of Escherichia coli by human neutrophils.
The in vitro effect of subinhibitory and inhibitory concentrations of ofloxacin and G-CSF on the bactericidal activity of polymorphonuclear leucocytes (PMNL) against Escherichia coli was investigated. PMNL obtained from healthy volunteers were incubated with different concentrations of G-CSF and ofloxacin for 180 min. The minimum inhibitory concentration (MIC) of ofloxacin and even 1/4 x MIC enhanced the bactericidal activity of PMNL. G-CSF at a concentration of 6,000 units/ml led to a significant improvement of the bactericidal activity of PMNL. The combination of 6,000 units/ml of G-CSF and ofloxacin in inhibitory as well as subinhibitory concentrations, however, showed a significant synergistic effect on the antibacterial activity of PMNL during the complete incubation period. Combinations of G-CSF and antibiotics could therefore be beneficial for infected patients, especially those with impaired cellular host defense.
Kropec
A
A
Institut für Umweltmedizin und Krankenhaushygiene, Universitätsklinikum Freiburg, Germany.
Lemmen
S W
SW
Grundmann
H J
HJ
Engels
I
I
Daschner
F D
FD
D000328:Adult; D002478:Cells, Cultured; D004926:Escherichia coli / Q000187:drug effects / Q000276:immunology*; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D006801:Humans; D008297:Male; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D015242:Ofloxacin / Q000494:pharmacology*
D016179:Granulocyte Colony-Stimulating Factor; D015242:Ofloxacin