title: Presentation of antigen by B cell subsets. II. The role of CD5 B cells in the presentation of antigen to antigen-specific T cells.
abstract: We demonstrate that peritoneal B cells have a much higher ability to present antigen to antigen-specific T cell lines than splenic B cells. Presentation of antigen by B cells is abrogated or drastically reduced after removal of Lyb-5+ cells from the population of splenic or peritoneal B cells. Peritoneal B cells, precultured for 7 days prior to the antigen presentation assay, retain their antigen presenting cell (APC) function. Enrichment for CD5+ cells in the peritoneal B cell population results in a more effective antigen presentation. Lastly, stimulation of B cells via CD5 antigen, by treatment of cells with anti-CD5 antibodies or cross-linking of CD5 receptors, enhances APC function of these cells. The results indicate, both indirectly and directly, that CD5+ B cells play a predominant role in the presentation of conventional antigens to antigen-specific T cells.
authors.affiliation: Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław.
authors.forename: M
authors.initials: M
authors.lastname: Zimecki
authors.forename: J A
authors.initials: JA
authors.lastname: Kapp
mesh_terms: D000818:Animals; D017951:Antigen Presentation / Q000276:immunology; D016175:B-Lymphocyte Subsets / Q000276:immunology*; D018956:CD5 Antigens / Q000276:immunology*; D000939:Epitopes; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008808:Mice, Inbred CBA; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D018956:CD5 Antigens; D000939:Epitopes