Fatty acid ethyl ester synthesis by human liver microsomes.
Fatty acid ethyl esters are a family of non-oxidative metabolites of ethanol present in many tissues after ethanol consumption. In this report we demonstrate the existence in human liver of an acyl-CoA:ethanol acyltransferase activity which may be responsible in part for the synthesis of these compounds in vivo. The effects of oleoyl-CoA and ethanol concentrations, presence or absence of bovine serum albumin and detergent, pH and enzyme concentration on this activity have been determined. Acyl-CoA:ethanol acyltransferase activity is localised in the membrane-bound fraction. Using inhibitors directed against related enzyme activities, it has been shown that the activity is not related to serine-dependent carboxylesterases or acyl-CoA:cholesterol acyltransferase, but tht it may be associated with acyl-CoA hydrolase activity. We have also compared acyl-CoA:ethanol acyltransferase activity with fatty acid ethyl ester synthase activity in microsomes and cytosol from the same liver. Our data indicate that these activities are comparable in vitro (on a unit/g liver basis), and suggest that both may be significant in vivo.
Treloar
T
T
Department of Biochemistry, University of Queensland, St. Lucia, Brisbane, Australia.
Madden
L J
LJ
Winter
J S
JS
Smith
J L
JL
de Jersey
J
J
D000214:Acyl Coenzyme A / Q000032:analysis; D000217:Acyltransferases / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000378:metabolism*; D000818:Animals; D004952:Esters / Q000378:metabolism*; D000431:Ethanol / Q000032:analysis; D005227:Fatty Acids / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D008862:Microsomes, Liver / Q000201:enzymology / Q000378:metabolism*; D009829:Oleic Acids / Q000096:biosynthesis; D010170:Palmitoyl-CoA Hydrolase / Q000378:metabolism; D051381:Rats
D000214:Acyl Coenzyme A; D004952:Esters; D005227:Fatty Acids; D009829:Oleic Acids; C017585:oleoyl-coenzyme A; D000431:Ethanol; D000217:Acyltransferases; C020124:ethanol acyltransferase; C043403:fatty acyl ethyl ester synthase; D010170:Palmitoyl-CoA Hydrolase; C033180:ethyl oleate