Circulating malignant lymphocytes from Sezary syndrome express high level of glycoproteins carrying beta (1-6)N-acetylglucosamine-branched N-linked oligosaccharides.
The circulating forms of malignant cells from patients with Sezary syndrome exhibit on their glycoproteins a high level of beta (1-6)GlcNAc-branched N-linked oligosaccharides, a particular species of glycans related to the metastatic potential of several tumors and T lymphocytes activation. An increased activity of the N-acetylglucosaminyltransferase V and of the beta (1-4)galactosyltransferase, two enzymes implicated in beta (1-6)GlcNAc-branching is also found. Nevertheless, contrary to activated normal T lymphocytes, Sezary lymphocytes in agreement with their non-proliferating state, do not exhibit increased thymidine uptake. This result suggests that expression of the beta (1-6)GlcNAc-branched N-linked carbohydrates could be related to some of the malignant properties of Sezary lymphocytes.
Derappe
C
C
Institut National de la Santé et de la Recherche Médicale U 180, Université Paris-V René Descartes, France.
Haentjens
G
G
LeMaire
S A
SA
Feugeas
J P
JP
Lebbe
C
C
Pasqualetto
V
V
Bussel
A
A
Aubery
M
M
Néel
D
D
D000117:Acetylglucosamine / Q000097:blood*; D005260:Female; D006023:Glycoproteins / Q000097:blood* / Q000737:chemistry; D006801:Humans; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D017351:N-Acetylglucosaminyltransferases / Q000378:metabolism; D009844:Oligosaccharides / Q000097:blood*; D012751:Sezary Syndrome / Q000097:blood* / Q000201:enzymology / Q000276:immunology; D012878:Skin Neoplasms / Q000097:blood* / Q000201:enzymology / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology; D006022:beta-N-Acetylglucosaminylglycopeptide beta-1,4-Galactosyltransferase / Q000378:metabolism
D006023:Glycoproteins; D009844:Oligosaccharides; D017351:N-Acetylglucosaminyltransferases; C052254:alpha-1,6-mannosylglycoprotein beta 1,6-N-acetylglucosaminyltransferase; D006022:beta-N-Acetylglucosaminylglycopeptide beta-1,4-Galactosyltransferase; D000117:Acetylglucosamine