title: Differential metabolism of exogenous platelet-activating factor by glandular epithelial and stromal cells of rabbit endometrium.
abstract: Significant changes in platelet-activating factor (PAF; 1-O-alkyl-2-acetyl-sn-glycero-3-phosphorylcholine) concentration have been observed in rabbit endometrium during the preimplantation period, but, under in vitro conditions, constitutive PAF biosynthesis by isolated endometrial tissues was not easily demonstrable. Relative changes in enzymes involved in the synthesis and metabolism of PAF in the tissues may account for this disparity. In addition, during this period of preimplantation, marked changes in PAF receptor concentration have been noted. The present study examines the factors that may modulate the metabolism of exogenous [3H]PAF in the endometrium of rabbits on day 6 of pregnancy. Since preferential [3H]PAF binding in situ by the glandular epithelial, but not by the stromal, cells was demonstrated, their cell-specific metabolism of exogenous [3H]PAF was also examined. After entry into the endometrial cell, [3H]PAF was rapidly metabolized by the sequential action of cytosolic Ca(2+)-independent acetylhydrolase to [3H]lyso-PAF and this was in turn acylated by membrane-associated transacylase to [3H]alkylacyl-glycerylphosphorylcholine. PAF resynthesis was not observed and, in stromal cells, there was a significant build-up of [3H]lyso-PAF, suggesting that lyso-PAF:acetyl-CoA acetyl-transferase may be a limiting factor. In the glandular epithelial cells, however, there was a significant accumulation of a neutral lipid without a significant build-up of [3H]lyso-PAF or [3H]PAF. The neutral lipid co-migrated with the product of phospholipase C-catalysed metabolism of PAF and authentic 1-O-hexadecyl-2-acetyl-glycerol. In addition, the elution times of phospholipase C digestion of C18 PAF and the neutral lipid produced by cellular metabolism of [3H]PAF, determined by gas chromatography/flame ionization detection, were similar. It seems that it is the synthesis of the neutral lipid from reacetylated [3H]lyso-PAF that prevented [3H]PAF accumulation under in vitro conditions. This is the first documentation of the synthesis of this lipid in the mammalian uterus. The lipid may serve as the precursor for de novo PAF synthesis in the glandular epithelial cells during endometrial proliferation.
authors.affiliation: Department of Clinical Laboratory Sciences, School of Allied Health Science, University of Texas Health Science Center, San Antonio 78284, USA.
authors.forename: G B
authors.initials: GB
authors.lastname: Kudolo
authors.forename: Y Q
authors.initials: YQ
authors.lastname: Yang
authors.forename: D B
authors.initials: DB
authors.lastname: Chen
authors.forename: M A
authors.initials: MA
authors.lastname: Jones
authors.forename: M J
authors.initials: MJ
authors.lastname: Harper
mesh_terms: D000123:Acetyltransferases / Q000378:metabolism; D000818:Animals; D002469:Cell Separation; D002478:Cells, Cultured; D002849:Chromatography, Gas; D002855:Chromatography, Thin Layer; D047108:Embryonic Development; D004717:Endometrium / Q000378:metabolism*; D004848:Epithelium / Q000378:metabolism; D005260:Female; D005410:Flame Ionization; D010742:Phospholipid Ethers / Q000378:metabolism / Q000494:pharmacology; D010972:Platelet Activating Factor / Q000031:analogs & derivatives / Q000378:metabolism / Q000493:pharmacokinetics*; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000378:metabolism*; D011817:Rabbits; D010738:Type C Phospholipases / Q000378:metabolism
chemical_list: C029271:O-deacetyl platelet activating factor; D010742:Phospholipid Ethers; D010972:Platelet Activating Factor; D000123:Acetyltransferases; C046035:1-alkylglycerophosphocholine acetyltransferase; D010738:Type C Phospholipases