title: Distribution of the basic fibroblast growth factor and its receptor gene expression in normal and degenerated rat intervertebral discs.
abstract: METHODS
  Using a rat spondylosis model, the distributions of cells expressing the basic fibroblast growth factor and its receptor were investigated in normal and degenerated intervertebral discs. Cell-proliferating activity in degenerated discs was also assessed.
  
  
  OBJECTIVE
  This study was conducted to determine whether basic fibroblast growth factor is related to intervertebral disc degeneration.
  
  
  BACKGROUND
  Basic fibroblast growth factor stimulates proliferation and matrix synthesis of cultured intervertebral disc cells.
  
  
  METHODS
  Immunohistochemistry and in situ hybridization histochemistry were conducted to detect cells with basic fibroblast growth factor-like immunoreactivity and fibroblast growth factor receptor messenger RNA, respectively. Cell-proliferating activity was evaluated by AgNOR staining.
  
  
  RESULTS
  In degenerated discs, round chondrocytes with basic fibroblast growth factor-like immunoreactivity and fibroblast growth factor receptor messenger RNA are scattered instead of spindle-shaped cells in the normal anulus (normal anular cells), which are devoid of basic fibroblast growth factor-like immunoreactivity and fibroblast growth factor receptor messenger RNA. The proliferating activity of these chondrocytes is suggested to exceed that of normal anular cells.
  
  
  CONCLUSIONS
  Basic fibroblast growth factor is suggested to promote proliferation of chondrocytes in degenerated discs in an autocrine or paracrine manner. Basic fibroblast growth factor may be related to intervertebral disc degeneration as a proliferation-stimulating factor of chondrocytes that replace normal anular cells during disc degeneration.
authors.affiliation: Department of Orthopaedic Surgery, Osaka University Medical School, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Nagano
authors.forename: K
authors.initials: K
authors.lastname: Yonenobu
authors.forename: S
authors.initials: S
authors.lastname: Miyamoto
authors.forename: M
authors.initials: M
authors.lastname: Tohyama
authors.forename: K
authors.initials: K
authors.lastname: Ono
mesh_terms: D000818:Animals; D002455:Cell Division; D002574:Cervical Vertebrae / Q000378:metabolism* / Q000473:pathology; D004195:Disease Models, Animal; D016222:Fibroblast Growth Factor 2 / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007403:Intervertebral Disc / Q000378:metabolism* / Q000473:pathology; D008297:Male; D012333:RNA, Messenger / Q000032:analysis*; D051381:Rats; D017208:Rats, Wistar; D017468:Receptors, Fibroblast Growth Factor / Q000235:genetics / Q000378:metabolism*; D013128:Spinal Osteophytosis / Q000378:metabolism* / Q000473:pathology; D013904:Thoracic Vertebrae / Q000378:metabolism* / Q000473:pathology
chemical_list: D012333:RNA, Messenger; D017468:Receptors, Fibroblast Growth Factor; D016222:Fibroblast Growth Factor 2