title: Analysis of amplicons containing the esterase genes responsible for insecticide resistance in the peach-potato aphid Myzus persicae (Sulzer).
abstract: The amplification of genes encoding an insecticide-detoxifying esterase (E4) in the peach-potato aphid Myzus persicae is one of the few examples where this genetic phenomenon has been shown to be involved in the response of an intact higher organism to artificial selection. Here we report quantitative and qualitative studies of the repeat units (amplicons) containing the E4 genes in a highly resistant aphid clone. Initial studies to quantify esterase sequences showed a 5-11-fold increase in resistant aphids compared with susceptible aphids, suggesting the presence of 10-22 gene copies per diploid genome. A more incisive analysis by pulsed-field gel electrophoresis confirmed the presence of about 12 copies of the E4 gene and showed them to be on about 24 kb amplicons, arranged as a tandem array of direct repeats. This, together with previous results from crossing experiments and with recent in situ hybridization studies, confirms that the E4 gene amplification in this aphid clone is heterozygous at a single locus. However, these data show that the gene amplification alone cannot account for the approx. 60 times higher levels of E4 protein and its mRNA present in this aphid clone, and therefore resistance must involve changes in both esterase gene copy number and gene expression.
authors.affiliation: IACR-Rothamsted, Harpenden, U.K.
authors.forename: L M
authors.initials: LM
authors.lastname: Field
authors.forename: A L
authors.initials: AL
authors.lastname: Devonshire
authors.forename: C
authors.initials: C
authors.lastname: Tyler-Smith
mesh_terms: D000818:Animals; D001042:Aphids / Q000235:genetics*; D015139:Blotting, Southern; D004247:DNA; D016521:Electrophoresis, Gel, Pulsed-Field; D004950:Esterases / Q000235:genetics*; D005784:Gene Amplification; D007305:Insecticide Resistance / Q000235:genetics*
chemical_list: D004247:DNA; D004950:Esterases