Occurrence of secretogranin II in the prolactin-immunoreactive neurons of the rat lateral hypothalamus: an in situ hybridization and immunocytochemical study.
The occurrence of secretogranin II in a neuron population of the rat lateral hypothalamus specifically detected by an anti-serum to ovine prolactin was examined. As this population was previously reported to synthesize dynorphin, the distribution of neurons recognized by ovine prolactin-, dynorphin B- and secretogranin II anti-sera was investigated on adjacent sections of hypothalami. The prolactin immunoreactive neurons were the only cells in the lateral hypothalamus to be stained by secretogranin II anti-serum. Moreover, coupling immunocytochemical detection and in situ hybridization with an oligonucleotide probe complementary to secretogranin II mRNA showed that these neurons expressed the secretogranin II gene. These new findings should help to study the physiological role of the prolactin immunoreactive neurons of the lateral hypothalamus.
Griffond
B
B
C.N.R.S. URA 561, Laboratoire d'Histologie Embryologie Cytogénétique, Faculté de Médecine, Institute d'Etudes et de Transfert de Gènes, Besançon, France.
Grillon
S
S
Duval
J
J
Colard
C
C
Jacquemard
C
C
Deray
A
A
Fellmann
D
D
D000818:Animals; D002864:Chromogranins; D004399:Dynorphins / Q000032:analysis / Q000235:genetics; D004723:Endorphins / Q000032:analysis / Q000235:genetics; D007031:Hypothalamus / Q000737:chemistry* / Q000166:cytology; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D009474:Neurons / Q000737:chemistry*; D010455:Peptides / Q000032:analysis; D011388:Prolactin / Q000235:genetics / Q000276:immunology*; D011506:Proteins / Q000032:analysis* / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley / Q000033:anatomy & histology*
D002864:Chromogranins; D004723:Endorphins; D010455:Peptides; D011506:Proteins; D012333:RNA, Messenger; D004399:Dynorphins; C036200:rimorphin; D011388:Prolactin