High levels of protection induced by a 40-mer synthetic peptide vaccine against the intestinal nematode parasite Trichinella spiralis.
Parenteral vaccination, using a 40-mer synthetic peptide from a 43,000 MW immunodominant glycoprotein secreted by the intestinal nematode parasite Trichinella spiralis, induced high levels of protection against a subsequent challenge infection in mice. The expulsion of adult worms from the gut was accelerated following vaccination with peptide 40-80, but peptides 81-120 and 121-160 were not protective. Mesenteric lymph node cells taken from T. spiralis-infected mice showed some proliferation in response to in vitro stimulation with peptide 40-80, but were unaffected by peptide 81-120. As expulsion of T. spiralis is known to be brought about by T-cell-mediated inflammatory events in the intestine, the ability of the 40-80 sequence to promote accelerated worm expulsion upon challenge infection may reflect induction of a specific and appropriate T-cell response. To our knowledge, this is the first report of a protective synthetic peptide vaccine against an intestinal nematode.
Robinson
K
K
Department of Life Science, University of Nottingham, UK.
Bellaby
T
T
Chan
W C
WC
Wakelin
D
D
D000595:Amino Acid Sequence; D000818:Animals; D000947:Antigens, Helminth / Q000737:chemistry / Q000276:immunology*; D002455:Cell Division / Q000276:immunology; D005260:Female; D008198:Lymph Nodes / Q000276:immunology; D008643:Mesentery; D051379:Mice; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D017160:Trichinella spiralis / Q000276:immunology*; D014235:Trichinellosis / Q000276:immunology / Q000517:prevention & control*; D014614:Vaccines, Synthetic / Q000737:chemistry / Q000276:immunology*
D000947:Antigens, Helminth; D014614:Vaccines, Synthetic