title: [Effect of omeprazole administration on pancreatic content of enzymes in rats].
abstract: Gastric chloride acid plays an important role in pancreatic enzyme synthesis and secretion, mediated by cholecystokinin released in the duodenum. This study was designed to evaluate the influence of gastric acid suppression by omeprazole on pancreatic enzyme content. Eighteen male Wistar rats (180-220 g) were divided in two groups: I--control and II--omeprazole. Animals received by intraduodenal catheter 3 doses of 0.5 ml saline solution (NaCl 0.9%)--Group I or 5 mumol/Kg of omeprazole solution--Group II at 24 h intervals. All animals, after an overnight fasting period, were killed 3 h after the last dose. Serum amylase and pancreatic tissue content of protein, trypsinogen, elastase, lipase and phospholipase A2 were determinated. Omeprazole treated animals (group II) showed statistically significant lower levels of serum amylase and pancreatic trypsinogen content (P < 0.05). We believe that this effect is related with acid secretion suppression by omeprazole and that it may be mediated by cholecystokinin.
authors.affiliation: Departamento de Gastroenterologia, Faculdade de Medicina, Universidade de São Paulo.
authors.forename: A L
authors.initials: AL
authors.lastname: Montagnini
authors.forename: M S
authors.initials: MS
authors.lastname: Kubrusly
authors.forename: A M
authors.initials: AM
authors.lastname: Coelho
authors.forename: N A
authors.initials: NA
authors.lastname: Molan
authors.forename: J E
authors.initials: JE
authors.lastname: da Cunha
authors.forename: M C
authors.initials: MC
authors.lastname: Machado
authors.forename: H W
authors.initials: HW
authors.lastname: Pinotti
mesh_terms: D000818:Animals; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D008297:Male; D009853:Omeprazole / Q000494:pharmacology*; D010179:Pancreas / Q000187:drug effects* / Q000201:enzymology*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D004791:Enzyme Inhibitors; D009853:Omeprazole
vernacular_title: Efeito da administração de omeprazol sobre o conteúdo pancreático de enzimas em ratos.