title: Region-specific alterations of calbindin-D28k immunoreactivity in the rat hippocampus following adrenalectomy and corticosterone treatment.
abstract: The aim of this study was (i) to compare the immunocytochemical distribution of the calcium-binding protein calbindin-D28k (CB) in the hippocampus of rats with the pattern of neurodegeneration following adrenalectomy (ADX) using silver impregnation, and (ii) to investigate the CB-immunoreactivity in the hippocampus following 3 weeks corticosterone treatment. 24 h following ADX no degenerative changes, nor alterations in CB-immunoreactivity were found in the hippocampus. Both 3 and 21 days following ADX neurodegeneration in the dentate gyrus could be observed which was accompanied with a loss of CB-immunoreactive (CB-ir) cells in that parts of the dentate gyrus suffering neuronal degeneration. Additionally we observed a marked loss of CB-ir in the CA1 area both 3 and 21 days following ADX. Three weeks daily corticosterone treatment (10 mg/day) induced a marked increase of CB-ir exclusively in the CA1 pyramidal cell layer. We conclude that (i) there is a close relationship between the loss of CB-immunoreactive cells in the DG and the neuronal degeneration in the dentate gyrus following ADX, and (ii) corticosterone appears to be involved in the regulation of calbindin-D28k in the CA1 pyramidal cell layer.
authors.affiliation: Department of Biological Psychiatry, University of Groningen, The Netherlands.
authors.forename: H J
authors.initials: HJ
authors.lastname: Krugers
authors.forename: R M
authors.initials: RM
authors.lastname: Medema
authors.forename: F
authors.initials: F
authors.lastname: Postema
authors.forename: J
authors.initials: J
authors.lastname: Korf
mesh_terms: D000315:Adrenalectomy; D000818:Animals; D064092:Calbindin 1; D064026:Calbindins; D003345:Corticosterone / Q000097:blood / Q000494:pharmacology*; D018891:Dentate Gyrus / Q000166:cytology / Q000378:metabolism; D006624:Hippocampus / Q000033:anatomy & histology / Q000187:drug effects / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D009410:Nerve Degeneration / Q000187:drug effects; D017966:Pyramidal Cells / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D064030:S100 Calcium Binding Protein G / Q000378:metabolism*; D016622:Silver Staining; D015430:Weight Gain / Q000187:drug effects
chemical_list: C576500:Calb1 protein, rat; D064092:Calbindin 1; D064026:Calbindins; D064030:S100 Calcium Binding Protein G; D003345:Corticosterone