[Construction and expression of the gene CD4V1V2-PE40, coding for a molecular targeted protein against AIDS].
We here replaced the cell-binding domain in Pseudomonas exotoxin A (PE) with HIV-binding portion of CD4V1V2 molecule and constructed a chimeric gene CD4V1V2-PE40, which can be expressed in E. coli. The hybrid protein displays targeting toxicity toward cells infected by HIV and thus represents a promising novel therapeutic agent for the treatment of AIDS.
Zhao
X
X
Institute of Medicinal Biotechnology, CAMS, Beijing.
Zhang
M
M
Li
H
H
Lu
S
S
D036002:ADP Ribose Transferases; D000163:Acquired Immunodeficiency Syndrome; D000998:Antiviral Agents / Q000494:pharmacology; D001427:Bacterial Toxins / Q000235:genetics; D015704:CD4 Antigens / Q000235:genetics*; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D005098:Exotoxins / Q000235:genetics*; D015870:Gene Expression; D006801:Humans; D010957:Plasmids; D011550:Pseudomonas aeruginosa; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis* / Q000494:pharmacology; D015302:Simian Immunodeficiency Virus / Q000187:drug effects; D037521:Virulence Factors; D000097668:Pseudomonas aeruginosa Exotoxin A
D036002:ADP Ribose Transferases; D000998:Antiviral Agents; D001427:Bacterial Toxins; D015704:CD4 Antigens; D005098:Exotoxins; D011993:Recombinant Fusion Proteins; D037521:Virulence Factors; D000097668:Pseudomonas aeruginosa Exotoxin A