title: Surface-modified poly(methyl methacrylate) enhances adsorption and retains anticandidal activities of salivary histatin 5.
abstract: Denture-induced stomatitis is a common intraoral disease which is associated with high levels of Candida albicans adhesion to a denture surface. The aim of this study was to produce a surface-modified denture resin, which is usually manufactured from poly(methyl methacrylate) (PMMA), carrying an immobilized anticandidal protein. PMMA was modified by surface polymerization of methyl methacrylic acid to enhance adsorption of a potent candidacidal salivary protein, histatin 5. The modified PMMA showed higher surface adsorption and desorption of histatin 5 than the unmodified material. Because histatin 5 destabilizes C. albicans cell membranes and allows efflux of intracellular molecules, candidacidal activity was monitored by dye release from fungal cells. Adsorbed histatin 5 did not release dye from the yeast cells; however, dye was detected as histatin was desorbed from the surface. In an adhesion assay, modified PMMA decreased human submandibular-sublingual saliva (HSMSL) mediated adherence of yeast cells to the polymer. Precoating histatin 5 onto unmodified PMMA also abolished HSMSL-mediated adhesion. These experiments show that dental acrylic may be surface modified and loaded with histatin 5 as a means of controlled release of histatin 5 to an affected area. This surface modification may additionally reduce adhesion of C. albicans cells to the saliva-coated material.
authors.affiliation: Department of Oral Biology, State University of New York at Buffalo 14214, USA.
authors.forename: M
authors.initials: M
authors.lastname: Edgerton
authors.forename: P A
authors.initials: PA
authors.lastname: Raj
authors.forename: M J
authors.initials: MJ
authors.lastname: Levine
mesh_terms: D000327:Adsorption; D000328:Adult; D000595:Amino Acid Sequence; D000935:Antifungal Agents / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D002176:Candida albicans / Q000187:drug effects*; D005260:Female; D005452:Fluoresceins / Q000378:metabolism; D054808:Histatins; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D008768:Methylmethacrylates; D008969:Molecular Sequence Data; D012471:Salivary Proteins and Peptides / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties
chemical_list: D000935:Antifungal Agents; D005452:Fluoresceins; C068075:HTN3 protein, human; D054808:Histatins; D008768:Methylmethacrylates; D012471:Salivary Proteins and Peptides; C007740:fluorexon