A simple method for synthesis of B-cell clonospecific probes.
A sensitive PCR-based method was developed to produce B-cell clonogenic probes without the need for sequencing and specific oligonucleotide synthesis. Specificity and sensitivity were assessed and found to be comparable to that achieved using established methods. Possible applications include the detection of MRD, bone marrow involvement with lymphoma, and the contamination of autologous bone marrow or peripheral blood progenitor cell harvests with malignant cells carrying IgH rearrangements.
Saal
R J
RJ
Department of Haematology, University of Queensland, Princess Alexandra Hospital, Brisbane, Australia.
Gordon
R B
RB
Cobcroft
R G
RG
Scott
D C
DC
D000208:Acute Disease; D001402:B-Lymphocytes / Q000276:immunology*; D001483:Base Sequence; D015342:DNA Probes; D015326:Gene Rearrangement, B-Lymphocyte, Heavy Chain; D006801:Humans; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000235:genetics; D008228:Lymphoma, Non-Hodgkin / Q000235:genetics; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
D015342:DNA Probes