Growth regulation of endothelial cells by tissue fragments: analysis on mechanism of rapid endothelialization of tissue-seeded vascular prosthesis in a three-dimensional culture system.
Rapid endothelialization of the inner surface was reported in an autologous tissue-seeded vascular prosthesis. We applied a three-dimensional in vitro culture system to elucidate the precise mechanism of rapid endothelial coverage of a tissue-seeded vascular prosthesis. Human venous, omental, adipose and striated muscle tissue fragments were harvested from surgical specimens. They were embedded in collagen gel, and 2.0 x 10(5) bovine aortic endothelial cells (BAECs) were seeded on the gel. The number of BAECs was counted on Days 2 and 7. Growth rate of BAECs was facilitated on the collagen gel with omental and striated muscle tissue fragments (p < 0.05). Factor VIII-negative spindle cells migrated around tissue fragments, especially around the omental and striated muscle tissue fragments. Rapid endothelialization of a tissue-seeded vascular prosthesis may result from facilitation of EC proliferation by viable tissue fragments and migrated cells. These results confirm tissue fragments regulate EC growth, and are useful as bioengineering tools.
Mo
M
M
First Department of Surgery, Yokohama City University, Japan.
Noishiki
Y
Y
Kitamura
H
H
Kurihara
T
T
Kosuge
T
T
Ichikawa
Y
Y
Imoto
K
K
Kondo
J
J
Matsumoto
A
A
D000818:Animals; D001011:Aorta / Q000166:cytology / Q000378:metabolism / Q000502:physiology; D001807:Blood Vessel Prosthesis / Q000009:adverse effects / Q000592:standards*; D002417:Cattle; D002455:Cell Division / Q000502:physiology; D003094:Collagen / Q000737:chemistry; D046508:Culture Techniques; D004730:Endothelium, Vascular / Q000166:cytology* / Q000502:physiology; D005782:Gels; D006801:Humans; D009131:Muscle, Smooth, Vascular / Q000378:metabolism / Q000502:physiology; D012501:Saphenous Vein / Q000378:metabolism / Q000502:physiology
D005782:Gels; D003094:Collagen