Electrochemical oxidation of CBS-113 A, a new anti-inflammatory drug: applicability to liquid chromatography-electrochemical detection.
The electrochemical properties of a new non-steroidal anti-inflammatory drug (2-hydroxy-4-methylphenyl-2-aminothiazole hydrochloride; CBS-113 A) have been studied using voltammetry in direct current and cyclic modes at glassy carbon disk electrodes. The results show an oxidative process with a diffusion-controlled and a reversible mechanism; these data agree with those obtained in a reversed-phase high-performance liquid chromatography (HPLC) system coupled either with amperometric (single glassy carbon electrode) or coulometric detection (two porous graphite electrodes in series) set at potentials of +0.65 V (vs. Ag/AgCl) and +0.4 V (vs. Pd/H2), respectively. Similar electrochemical properties were found for 2-hydroxyphenyl-2-amino-5'-methylthiazole hydrochloride (RD-1546) which is a potential internal standard. An HPLC system coupled with a UV detector (lambda = 272 nm) and an amperometric detector (+0.65 V) showed a gain in sensitivity of about 10 using electrochemical detection (ED) for the determination of CBS-113 A in human plasma. Linearity range, precision and accuracy were calculated and showed the potential application of HPLC-ED to pharmacokinetic studies of CBS-113 A in plasma.
Leroy
P
P
Laboratoire de Chimie Analytique, URA CNRS 597, Faculté des Sciences Pharmaceutiques et Biologiques, Nancy, France.
Baudrit
O
O
Blanchin
M D
MD
Nicolas
A
A
Fabre
H
H
D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000032:analysis* / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004563:Electrochemistry; D016166:Free Radical Scavengers / Q000032:analysis / Q000737:chemistry*; D006801:Humans; D010084:Oxidation-Reduction; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet; D013844:Thiazoles / Q000032:analysis / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics
D000894:Anti-Inflammatory Agents, Non-Steroidal; D016166:Free Radical Scavengers; D013844:Thiazoles; C062488:2-(2-hydroxy-4-methylphenyl)aminothiazole