title: Expression of the common cytokine receptor gamma chain by murine dendritic cells including epidermal Langerhans cells.
abstract: The common cytokine receptor gamma chain (gamma c) is an indispensable component of interleukin (IL)-2, IL-4, IL-7, IL-9, and IL-15 receptors, and its expression has been detected in several leukocyte populations, including T cells, B cells, monocytes, natural killer cells, and neutrophils. The purpose of this study was to determine whether gamma c receptors are expressed by dendritic cells (DC). Constitutive gamma c mRNA expression was observed by reverse transcription polymerase chain reaction and/or Northern blotting for: (a) Ia+ epidermal Langerhans cells (LC), (b) 4F7+ splenic DC, (c) granulocyte/macrophage colony-stimulated factor-propagated bone marrow-derived DC, and (d) the epidermal-derived DC line, XS52, which retains important functions of epidermal LC. Exposure of XS52 cells to recombinant IL-4 induced a rapid up-regulation of c-myc mRNA expression, and this IL-4-dependent signaling was blocked almost completely by anti-gamma c monoclonal antibody (mAb) TUGm2 in a soluble form. Moreover, c-myc up-regulation was inducible in XS52 cells by the same mAb in an immobilized form. These results imply that molecules recognized by this antibody (i.e. gamma c receptors) are expressed on XS52 cell surfaces. We thus conclude that DC express functional gamma c receptors, which then mediate cytokine-dependent regulation of DC functions.
authors.affiliation: Department of Dermatology, University of Texas Southwestern Medical Center, Dallas 75235, USA.
authors.forename: M
authors.initials: M
authors.lastname: Mohamadzadeh
authors.forename: K
authors.initials: K
authors.lastname: Ariizumi
authors.forename: K
authors.initials: K
authors.lastname: Sugamura
authors.forename: P R
authors.initials: PR
authors.lastname: Bergstresser
authors.forename: A
authors.initials: A
authors.lastname: Takashima
mesh_terms: D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003713:Dendritic Cells / Q000145:classification / Q000378:metabolism*; D004817:Epidermis / Q000276:immunology*; D005260:Female; D007801:Langerhans Cells / Q000378:metabolism*; D008565:Membrane Proteins / Q000096:biosynthesis; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000096:biosynthesis; D018121:Receptors, Cytokine / Q000032:analysis / Q000235:genetics / Q000378:metabolism*
chemical_list: D008565:Membrane Proteins; D012333:RNA, Messenger; D018121:Receptors, Cytokine