title: Controlled clinical evaluation of BACTEC Plus Aerobic/F and BacT/Alert Aerobic FAN bottles for detection of bloodstream infections.
abstract: A total of 7,190 blood culture sets were obtained from adult patients with a suspected bloodstream infection. A 20-ml sample of blood was distributed equally between the aerobic FAN bottle which was monitored in the BacT/Alert system and a Plus Aerobic/F bottle which was monitored in the BACTEC 9240 system. A total of 988 positive cultures were obtained from 483 patients; however, only 453 positive cultures from 173 patients met the criteria for volume ( > or = ml per bottle) and clinical significance on the basis of concurrent case review required for data analysis. There were 25 and 68 false positives from the FAN and Plus Aerobic/F bottles, respectively. There were no statistically significant differences between systems in the number of positive cultures or septic episodes by species; however, the total number of Enterobacteriaceae and Pseudomonas aeruginosa isolates combined was significantly greater in the FAN bottle (P = 0.04). Detection times did not differ significantly between systems for positive cultures; however, episodes of Staphylococcus aureus bacteremia were detected significantly more rapidly from the FAN bottle (P = 0.005). There was no significant difference between systems in the detection of bloodstream infections in patients receiving antibiotics at the time of blood culture.
authors.affiliation: Department of Clinical Pathology, Cleveland Clinic Foundation, Ohio 44195, USA.
authors.forename: J K
authors.initials: JK
authors.lastname: Pohlman
authors.forename: B A
authors.initials: BA
authors.lastname: Kirkley
authors.forename: K A
authors.initials: KA
authors.lastname: Easley
authors.forename: B A
authors.initials: BA
authors.lastname: Basille
authors.forename: J A
authors.initials: JA
authors.lastname: Washington
mesh_terms: D000328:Adult; D000332:Aerobiosis; D016470:Bacteremia / Q000175:diagnosis*; D001419:Bacteria / Q000254:growth & development*; D003470:Culture Media; D005189:False Positive Reactions; D016469:Fungemia / Q000175:diagnosis*; D016905:Gram-Negative Bacterial Infections / Q000175:diagnosis; D016908:Gram-Positive Bacterial Infections / Q000175:diagnosis; D006801:Humans; D008828:Microbiological Techniques / Q000295:instrumentation*; D013997:Time Factors; D015003:Yeasts / Q000254:growth & development*
chemical_list: D003470:Culture Media