title: Normal host prion protein necessary for scrapie-induced neurotoxicity.
abstract: Accumulation of the prion protein PrPSc, a pathological and protease-resistant isoform of the normal host protein PrPC, is a feature of prion disease such as scrapie. It is still unknown whether scrapie pathology comes about by neurotoxicity of PrPSc, acute depletion of PrPC, or some other mechanism. Here we investigate this question by grafting neural tissue overexpressing PrPC into the brain of PrP-deficient mice which are scrapie-resistant and do not propagate infectivity. After intracerebral inoculation with scrapie prions, the grafts accumulated high levels of PrPSc and infectivity and developed the severe histopathological changes characteristic of scrapie. Moreover, substantial amounts of graft-derived PrPSc migrated into the host brain. Even 16 months after inoculation no pathological changes were seen in PrP-deficient tissue, not even in the immediate vicinity of the grafts. Therefore, in addition to being resistant to scrapie infection, brain tissue devoid of PrPC is not damaged by exogenous PrPSc.
authors.affiliation: Department of Pathology, University Hospital, Zürich, Switzerland.
authors.forename: S
authors.initials: S
authors.lastname: Brandner
authors.forename: S
authors.initials: S
authors.lastname: Isenmann
authors.forename: A
authors.initials: A
authors.lastname: Raeber
authors.forename: M
authors.initials: M
authors.lastname: Fischer
authors.forename: A
authors.initials: A
authors.lastname: Sailer
authors.forename: Y
authors.initials: Y
authors.lastname: Kobayashi
authors.forename: S
authors.initials: S
authors.lastname: Marino
authors.forename: C
authors.initials: C
authors.lastname: Weissmann
authors.forename: A
authors.initials: A
authors.lastname: Aguzzi
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism / Q000473:pathology; D016380:Brain Tissue Transplantation; D007124:Immunoenzyme Techniques; D051379:Mice; D008822:Mice, Transgenic; D018621:PrPC Proteins / Q000235:genetics / Q000378:metabolism*; D018620:PrPSc Proteins / Q000378:metabolism / Q000633:toxicity*; D012608:Scrapie / Q000209:etiology* / Q000378:metabolism / Q000473:pathology
chemical_list: D018621:PrPC Proteins; D018620:PrPSc Proteins