title: Phospholipid profiling of sediments using phosphorus-31 nuclear magnetic resonance.
abstract: A phosphorus-31 nuclear magnetic resonance method has been developed for the determination of aquatic sediment phospholipid profiles that may be generally applied to all soils and deposits containing viable cellular material. A method of scrubbing chloroform/methanol extracts with potassium acid phosphate overcomes adverse signal broadening from the mineral component, permitting eleven sediment phospholipids to be determined at the quantitative level.
authors.affiliation: Center for Great Lakes Studies, University of Wisconsin-Milwaukee 53204, USA.
authors.forename: L G
authors.initials: LG
authors.lastname: Bardygula-Nonn
authors.forename: J L
authors.initials: JL
authors.lastname: Kaster
authors.forename: T
authors.initials: T
authors.lastname: Glonek
mesh_terms: D002725:Chloroform; D005990:Glycerol / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D000432:Methanol; D010710:Phosphates; D010743:Phospholipids / Q000032:analysis; D010759:Phosphorus Isotopes; D010955:Plasmalogens / Q000032:analysis; D017680:Potassium Compounds; D012680:Sensitivity and Specificity; D012987:Soil / Q000032:analysis*; D014867:Water / Q000032:analysis
chemical_list: D010710:Phosphates; D010743:Phospholipids; D010759:Phosphorus Isotopes; D010955:Plasmalogens; D017680:Potassium Compounds; D012987:Soil; D014867:Water; D002725:Chloroform; C013216:potassium phosphate; D005990:Glycerol; D000432:Methanol