title: Search for antibodies for immunoenzymatic assay of human lutropin.
abstract: Five peptides corresponding to human lutropin (hLH) subunit fragments were synthesized by a solid phase method and their physicochemical characteristics are presented. Antibodies induced in rabbits with 3 peptides of beta hLH fragments coupled to thyrotropin did not bind hLH and human chorionic gonadotropin (hCG). Also 2 synthetic peptides of alpha hLH fragments did not react with rabbit anti-hLH, anti-hCG and anti-alpha hCG antibodies. Using 2 monoclonal anti-alpha hCG and anti-beta hLH antibodies a sensitive sandwich ELISA technique was elaborated. Using this technique stability of hLH was investigated. The ELISA was also applied for assays of urine hLH in normal and ovulation days.
authors.affiliation: Biochemical Laboratory, Polish Academy of Sciences, Wrocław.
authors.forename: A
authors.initials: A
authors.lastname: Szewczuk
authors.forename: M
authors.initials: M
authors.lastname: Kuropatwa
authors.forename: I
authors.initials: I
authors.lastname: Kochanowska
authors.forename: A
authors.initials: A
authors.lastname: Majewska
authors.forename: Z
authors.initials: Z
authors.lastname: Maćkiewicz
authors.forename: G
authors.initials: G
authors.lastname: Kupryszewski
authors.forename: A
authors.initials: A
authors.lastname: Rapak
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies; D000911:Antibodies, Monoclonal; D000917:Antibody Formation; D000918:Antibody Specificity; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004355:Drug Stability; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D007986:Luteinizing Hormone / Q000032:analysis* / Q000737:chemistry / Q000652:urine; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D010060:Ovulation; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000737:chemistry / Q000276:immunology; D011817:Rabbits; D012996:Solutions
chemical_list: D000906:Antibodies; D000911:Antibodies, Monoclonal; D046911:Macromolecular Substances; D010446:Peptide Fragments; D012996:Solutions; D007986:Luteinizing Hormone