Isolation and characterization of Locusta migratoria accessory gland myotropin I (Lom-Ag-MT-I) from the brain of the Colorado potato beetle, Leptinotarsa decemlineata.
A novel myotropic Colorado potato beetle peptide, active in the Locusta oviduct motility assay, was isolated from a methanolic extract of 9,000 brain complexes of adult Leptinotarsa decemlineata by means of HPLC. Its sequence is Gly-Phe-Lys-Asn-Val-Ala-Leu-Ser-Thr-Ala-Arg-Gly-Phe-NH2. This peptide is identical to Lom-AG-MT-I, a myotropin previously isolated from the male accessory glands of Locusta migratoria, using the L. migratoria oviduct motility bioassay as a monitoring system. It strongly stimulated the frequency, amplitude, and tonus of the myogenic oviduct contractions, even at low concentrations.
Spittaels
K
K
Zoologisch Instituut, Katholieke Universiteit Leuven, Belgium.
Vankeerberghen
A
A
Schoofs
L
L
Proost
P
P
Van Damme
J
J
De Loof
A
A
D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain; D002851:Chromatography, High Pressure Liquid; D001517:Coleoptera; D003120:Colorado; D005260:Female; D005837:Genitalia, Male / Q000737:chemistry; D006110:Grasshoppers; D008297:Male; D008969:Molecular Sequence Data; D009420:Nervous System / Q000737:chemistry*; D009479:Neuropeptides / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology; D010057:Oviducts / Q000187:drug effects / Q000502:physiology; D017386:Sequence Homology, Amino Acid
D009479:Neuropeptides; C068978:Lom-AG-myotropin