Comparison of receptor-binding properties among influenza C virus isolates.
A total of 10 influenza C virus strains isolated recently in Yamagata City, Japan and shown to belong to the same lineage was compared for the ability to agglutinate chicken and mouse erythrocytes under various conditions. C/Yamagata/10/89 was unique in lacking the ability to agglutinate chicken erythrocytes at a temperature > or = 4 degrees C. This isolate also agglutinated native mouse erythrocytes only very inefficiently, although the high agglutination titer was obtained with the glutaraldehyde-fixed cells. Furthermore, it was found that C/Yamagata/4/88, unlike the other isolates, agglutinated erythrocytes from chickens to lower titers than those from mice, even when assayed at 0 degree C. Comparison of the deduced amino acid sequence of hemagglutinin-esterase among the 6 representative strains including two older isolates, C/Yamagata/26/81 and C/Nara/2/85, suggested that the failures of C/Yamagata/10/89 to agglutinate chicken erythrocytes at > or = 4 degrees C and unfixed mouse erythrocytes to high titers may be due to amino acid changes at residues 337 (Glu-->Lys) and 340 (Thr-->Tyr), respectively, and that a change at residue 347 (Leu-->Ser) may be responsible for the decreased ability of C/Yamagata/4/88 to agglutinate chicken erythrocytes.
Matsuzaki
Y
Y
Department of Bacteriology, Yamagata University, School of Medicine, Japan.
Matsuzaki
M
M
Muraki
Y
Y
Sugawara
K
K
Hongo
S
S
Kitame
F
F
Nakamura
K
K
D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D002645:Chickens; D004912:Erythrocytes / Q000821:virology*; D006023:Glycoproteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D006384:Hemagglutination; D006389:Hemagglutinins, Viral / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D006801:Humans; D009982:Gammainfluenzavirus / Q000302:isolation & purification* / Q000378:metabolism; D051379:Mice; D011991:Receptors, Virus / Q000378:metabolism*; D013329:Structure-Activity Relationship; D014760:Viral Fusion Proteins; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
D000596:Amino Acids; D006023:Glycoproteins; D006389:Hemagglutinins, Viral; D011991:Receptors, Virus; D014760:Viral Fusion Proteins; D014764:Viral Proteins; C060814:hemagglutinin esterase