Isolation of the insecticidal components of Tagetes minuta (Compositae) against mosquito larvae and adults.
Application of Tagetes minuta floral extract to silica gel column chromatography produced 2 fractions with the hydrogenate part 20-30 times more toxic to larvae and 12-13 times more toxic to adults of Aedes aegypti and Anopheles stephensi, respectively, than the oxygenate part. Further fractionation by column chromatography of the hydrogenate fraction produced 4 thiophenes, 5-(but-3-ene-1-ynyl)-2,2'-bithiophene, 5-(but-3-ene-1-ynyl)-5'-methyl-2,2'-bithiophene, 2,2',5',2"-terthiophene, and 5-methyl-2,2',5',2"-terthiophene. These compounds in Tagetes minuta are largely responsible for the toxicity exhibited against the tested mosquitoes.
Perich
M J
MJ
Department of Entomology, Walter Reed Army Institute of Research, Washington, DC 20307-5100, USA.
Wells
C
C
Bertsch
W
W
Tredway
K E
KE
D000330:Aedes; D000818:Animals; D000852:Anopheles; D001681:Biological Assay; D005260:Female; D007306:Insecticides / Q000302:isolation & purification*; D007814:Larva; D008297:Male; D009032:Mosquito Control; D010936:Plant Extracts / Q000737:chemistry; D010938:Plant Oils / Q000737:chemistry; D013876:Thiophenes / Q000302:isolation & purification / Q000494:pharmacology*
D007306:Insecticides; D010936:Plant Extracts; D010938:Plant Oils; D013876:Thiophenes