Tectorial membrane regeneration in acoustically damaged birds: an immunocytochemical technique.
A novel immunocytochemical method was used to determine whether the sound-damaged adult quail ear can repair its tectorial membrane (TM) and to compare the repair in quail to that in chicks. Birds were exposed to an octave band noise with a center frequency of 1.5 kHz at 116 dB SPL for 4 h. The chicks were grouped based on recovery duration (0 and 7 days), while the quail were divided into 0-, 7-, and 14-day recovered groups. At the end of the recovery period, the animals were sacrificed, and their basilar papillae labeled with a TM-specific monoclonal primary antibody solution followed by a diaminobenzidine process. Examinations under a stereoscope revealed that a patch lesion devoid of TM was located on all 0-day recovered papillae. Seven days later, a honeycomb-patterned layer was observed covering the lesion. In 14-day recovered quail ears, the honeycomb layer appeared similar to that seen at 7 days post-exposure. These observations indicated that both chicks and quail were able to repair their TM within 7 days following exposure to intense sound.
Adler
H J
HJ
Kresge Hearing Research Institute, University of Michigan, Ann Arbor 48109, USA.
Niemiec
A J
AJ
Moody
D B
DB
Raphael
Y
Y
D000161:Acoustic Stimulation / Q000009:adverse effects*; D000818:Animals; D000831:Animals, Newborn; D000911:Antibodies, Monoclonal; D001489:Basilar Membrane / Q000473:pathology / Q000502:physiology / Q000648:ultrastructure; D002645:Chickens; D003370:Coturnix / Q000502:physiology*; D006316:Hearing Loss, High-Frequency / Q000503:physiopathology*; D007150:Immunohistochemistry; D008855:Microscopy, Electron, Scanning; D012038:Regeneration; D013045:Species Specificity; D013680:Tectorial Membrane / Q000473:pathology / Q000502:physiology* / Q000648:ultrastructure
D000911:Antibodies, Monoclonal