title: Glucosamine 6-phosphate deaminase in Plasmodium falciparum.
abstract: The pathways of glucose utilization for energy production in the malaria parasite, Plasmodium falciparum, have been studied extensively. Little is known, however, about the reactions by which glucose is converted into complex carbohydrates in the parasite, and knowledge of the catabolism of these substances is likewise scanty. The present investigation was undertaken to determine whether the parasites possess a key enzyme of glucosamine catabolism, i.e. glucosamine 6-phosphate deaminase (EC 5.3.1.40), which catalyses the conversion of the sugar phosphate to fructose 6-phosphate and ammonia. Lysates of Plasmodium-infected erythrocytes had substantially higher deaminase activity than control samples from normal erythrocytes, and an even higher specific activity was observed in extracts of isolated parasites, amounting to 20-40 times that of uninfected cells. Anion exchange chromatography indicated that the parasite deaminase eluted in a retarded position when compared to the elution profile of the erythrocyte enzyme. The charge difference suggested by these findings was established more directly by chromatofocusing, which indicated pI values of 6.85 and 8.55 for the parasite and erythrocyte deaminases, respectively. Other differences were also observed, notably a greater thermolability on the part of the parasite enzyme. These results indicated that the parasites synthesize a specific deaminase that is distinct from the normal erythrocyte enzyme. Studies on synchronized parasite cultures further indicated that the parasite deaminase is developmentally regulated, because a dramatic increase in activity levels occurred during the later stages of parasite development.
authors.affiliation: Department of Microbiology, School of Medicine, University of Alabama at Birmingham, USA.
authors.forename: J A
authors.initials: JA
authors.lastname: Weidanz
authors.forename: P
authors.initials: P
authors.lastname: Campbell
authors.forename: D
authors.initials: D
authors.lastname: Moore
authors.forename: L J
authors.initials: LJ
authors.lastname: DeLucas
authors.forename: L
authors.initials: L
authors.lastname: Rodén
authors.forename: A C
authors.initials: AC
authors.lastname: Vezza
mesh_terms: D019747:Aldose-Ketose Isomerases; D000818:Animals; D002238:Carbohydrate Epimerases / Q000378:metabolism*; D002852:Chromatography, Ion Exchange; D004912:Erythrocytes / Q000469:parasitology; D010963:Plasmodium falciparum / Q000201:enzymology*
chemical_list: C020582:glucosamine-6-phosphate isomerase; D002238:Carbohydrate Epimerases; D019747:Aldose-Ketose Isomerases