title: Fc gamma receptor expression on sheep afferent lymph dendritic cells and rapid modulation of cell surface phenotype following Fc gamma receptor engagement in vitro and in vivo.
abstract: Afferent lymph dendritic cells were analysed for the presence of Fc gamma receptors by Western blotting and for modulation of surface markers following Fc gamma receptor engagement in vitro and in vivo. The results showed that unstimulated dendritic cells expressed Fc gamma RII constitutively. When dendritic cells were incubated in vitro with antigen/antibody complexes in antibody excess, a marked reduction in surface staining was observed for MHC class II, CD1, CD44, and VLA-4 after 8 h in culture. These changes did not occur with antigen or antibody alone. DC expression of LFA-1 and LFA-3 were slightly reduced after 8 h in culture with Ova alone, but this was enhanced slightly when the cells were cultured with immune complexes. Even more marked reductions in surface staining for MHC class II, CD1, CD44 and VLA-4 were observed on dendritic cells 4-8 h following secondary antigen challenge in vivo. LFA-1 and LFA-3 expression was reduced only slightly. The level of expression of MHC class II, CD1, LFA-1 and LFA-3 was substantially increased over resting values 24 h after Fc gamma R occupancy. The intensity of staining at this time was also significantly elevated for CD44, LFA-1, LFA-3 and VLA-4. These results show that engagement of Fc gamma receptors cause a substantial modulation of the dendritic cell surface phenotype after immune complex uptake. The phenomenon may function to maximize subsequent presentation of the challenge antigen to T cells.
authors.affiliation: Department of Veterinary Pathology, University of Edinburgh, UK.
authors.forename: S N
authors.initials: SN
authors.lastname: Coughlan
authors.forename: G D
authors.initials: GD
authors.lastname: Harkiss
authors.forename: L
authors.initials: L
authors.lastname: Dickson
authors.forename: J
authors.initials: J
authors.lastname: Hopkins
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000276:immunology; D000954:Antigens, Surface / Q000276:immunology*; D015153:Blotting, Western; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000276:immunology*; D005434:Flow Cytometry; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D016130:Immunophenotyping; D008196:Lymph / Q000166:cytology; D008264:Macrophages / Q000276:immunology; D010047:Ovalbumin / Q000276:immunology; D017452:Receptors, IgG / Q000096:biosynthesis*; D015842:Serine Proteinase Inhibitors / Q000494:pharmacology; D012709:Serum Albumin / Q000276:immunology; D012756:Sheep / Q000276:immunology*
chemical_list: D015703:Antigens, CD; D000954:Antigens, Surface; D000949:Histocompatibility Antigens Class II; D007074:Immunoglobulin G; D017452:Receptors, IgG; D015842:Serine Proteinase Inhibitors; D012709:Serum Albumin; D010047:Ovalbumin