Heterologous protein secretion by Aspergillus niger growing in submerged culture as dispersed or aggregated mycelia.
The secreted production of a heterologous enzyme, hen egg-white lysozyme, by Aspergillus niger was studied in shake flasks containing media of different initial viscosities. Raising the viscosity of the medium by addition of polyvinylpyrrolidone (PVP) brought about a transition in the form of growth from aggregated mycelia (pellets) to dispersed mycelia. The specific yield of lysozyme in cultures containing an initial concentration of 5% (w/v) starch was 8 mg lysozyme/g dry weight. Addition of 2% (w/v) PVP to the medium resulted in a specific yield of 14 mg lysozyme/g dry weight.
Archer
D B
DB
Institute of Food Research, Colney, Norwich, UK.
MacKenzie
D A
DA
Ridout
M J
MJ
D001234:Aspergillus niger / Q000254:growth & development* / Q000378:metabolism; D003470:Culture Media; D009113:Muramidase / Q000378:metabolism*; D011205:Povidone / Q000494:pharmacology; D014783:Viscosity
D003470:Culture Media; D009113:Muramidase; D011205:Povidone