[Organization of ribosomal DNA from the phytoflagellates Astasia longa and Euglena gracilis: comparison of the structure of 19S and 28S rRNA genes].
Restriction maps of Astasia longa and Euglena gracilis var. bacillaris were built and localization of 5.8S, 19S and 28S rRNA genes was established on them by blot-hybridization method. In the A. longa and E. gracilis plasmid rDNA three intergeneous regions were found, two of which were intergeneous transcribed spacers flanking 5.8S RNA gene, and the third region seems to be an untranscribed spacer. Localization of 9 primers from E. gracilis was established on A. longa 19S rRNA gene by PCR; it was similar to E. gracilis. Using amplified A. longa 19S rRNA gene (2300 bp) we have determined the sequence of its 3'-region, which showed 87% homology with the same region of E. gracilis. Using plasmid pA1 S3-H1 containing S3-H1 fragment of A. longa rDNA the sequence of 28S rRNA gene 3'-region was determined. This sequence includes regions homologous to corresponding regions of E. gracilis Z: 6 nucleotides of 12/13 internal spacer, complete 13/14 internal spacer (86 bp), genes for 13th and 14th 28S rRNA components (56 and 86 bp) with approximately 90% of homology with E. gracilis Z, and fragment of untranscribed spacer (136 bp) with approximately 70% homology. It was shown that 28S rRNA genes of A. longa and E. gracilis have similar structure. Our data allow to conclude that these phytoflagellats are closely related.
Zaĭtseva
G N
GN
Oparina
N Iu
NIu
Kagramanova
V V
VV
Kleshchenko
E V
EV
D000818:Animals; D001483:Base Sequence; D004275:DNA, Ribosomal / Q000235:genetics*; D005056:Euglena gracilis / Q000235:genetics*; D016822:Euglenida / Q000235:genetics*; D008969:Molecular Sequence Data; D010957:Plasmids; D016133:Polymerase Chain Reaction; D012335:RNA, Ribosomal / Q000235:genetics*; D012339:RNA, Ribosomal, 28S / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
D004275:DNA, Ribosomal; D012335:RNA, Ribosomal; D012339:RNA, Ribosomal, 28S; C097639:RNA, ribosomal, 19S
Organizatsiia ribosomnoĭ DNK fitoflagelliat Astasia longa i Euglena gracilis: sravnenie struktury genov 19S i 28S rRNK.