title: Culturing satellite cells from living single muscle fiber explants.
abstract: Conventional methods for isolating myogenic (satellite) cells are inadequate when only small quantities of muscle, the tissue in which satellite cells reside, are available. We have developed a tissue culture system that reliably permits isolation of intact, living, single muscle fibers with associated satellite cells from predominantly fast and slow muscles of rat and mouse; maintenance of the isolated fibers in vitro; dissociation, proliferation, and differentiation of satellite cells from each fiber; and removal of the fiber from culture for analysis.
authors.affiliation: Muscle Cell Biology Group, Royal Postgraduate Medical School, Hammersmith Hospital, London, England.
authors.forename: J D
authors.initials: JD
authors.lastname: Rosenblatt
authors.forename: A I
authors.initials: AI
authors.lastname: Lunt
authors.forename: D J
authors.initials: DJ
authors.lastname: Parry
authors.forename: T A
authors.initials: TA
authors.lastname: Partridge
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D002469:Cell Separation / Q000379:methods; D005260:Female; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018101:Mice, Inbred mdx; D018485:Muscle Fibers, Skeletal / Q000166:cytology*; D051381:Rats; D017208:Rats, Wistar