Direct detection of disomy in human sperm by the PRINS technique.
We report the use of the PRimedIN Situ (PRINS) labelling technique for the direct estimation of disomy rates for various chromosomes in human sperm. The PRINS technique provides a rapid and reliable method for chromosome screening since specific labelling may be obtained in less than 2 h. In the present study, the disomy rates of chromosomes 2, 5, 9, 12 and 18 were investigated. The incidences of disomy are similar for all these chromosomes, ranging from 0.27% to 0.33%. These findings suggest an equal distribution of aneuploidies among autosomes in human sperm.
Pellestor
F
F
CNRS UPR 9008, BP 5051, Montpellier, France.
Quennesson
I
I
Coignet
L
L
Girardet
A
A
Andréo
B
B
Charlieu
J P
JP
D000782:Aneuploidy; D001483:Base Sequence; D002874:Chromosome Mapping; D002881:Chromosomes, Human, Pair 12; D002887:Chromosomes, Human, Pair 18; D002889:Chromosomes, Human, Pair 2; D002895:Chromosomes, Human, Pair 5; D002899:Chromosomes, Human, Pair 9; D017931:DNA Primers; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008969:Molecular Sequence Data; D013094:Spermatozoa / Q000166:cytology*
D017931:DNA Primers