Catecholamine and MHPG plasma levels, platelet MAO activity, and 3H-imipramine binding in heroin and cocaine addicts.
This work evaluated in a population of heroin and heroin plus cocaine human addicts: 1. Norepinephrine (NE), epinephrine (Epi) and 3-methoxy-4-hydroxyphenylglycol (MHPG) (the principal metabolite of brain NE) plasma levels; 2. Monoamine oxidase (MAO) activity; and 3. 3H-imipramine specific binding to the amine carrier in platelets. NE plasma levels were significantly lower in the short-term heroin user groups (1-3 and 4-6 yr), a finding not observed in both long-term heroin user ( > 6 yr) and heroin plus cocaine user ( > 6 yr) groups. Epi levels changed in a similar manner, except that a significant increase was noted in heroin plus cocaine abusers. Conversely, dopamine and MHPG plasma levels increased with the duration of heroin use, and even more with cocaine abuse. Platelet MAO activity increased in all groups. Specific 3H-imipramine binding sites showed an increase after 3 yr of heroin abuse and in all heroin plus cocaine addicts. In conclusion, short-term use of heroin decreases NE or Epi release, but with prolonged use, a slow adaptation occurs. In contrast, cocaine inhibits the neuronal Epi uptake, even in a situation of long duration of abuse. Probably the amine levels additionally regulate the amine carrier, resulting in changes that show a different pattern from major depression. These drugs of abuse may also influence directly or indirectly related enzymatic systems.
Macedo
T
T
Institute of Pharmacology and Experimental Therapeutics, Faculty of Medicine, University of Coimbra, Portugal.
Ribeiro
C A
CA
Cotrim
D
D
Tavares
P
P
Morgadinho
M T
MT
Caramona
M
M
Vicente
M T
MT
Rodrigues
L
L
Cardoso
M G
MG
Keating
M L
ML
D000328:Adult; D001792:Blood Platelets / Q000201:enzymology*; D002352:Carrier Proteins / Q000378:metabolism; D003042:Cocaine; D004837:Epinephrine / Q000097:blood*; D005260:Female; D006556:Heroin Dependence / Q000097:blood* / Q000201:enzymology; D006801:Humans; D007099:Imipramine / Q000097:blood*; D008297:Male; D008734:Methoxyhydroxyphenylglycol / Q000097:blood*; D008995:Monoamine Oxidase / Q000097:blood*; D009638:Norepinephrine / Q000097:blood*; D011955:Receptors, Drug / Q000378:metabolism; D012016:Reference Values; D019966:Substance-Related Disorders / Q000097:blood* / Q000201:enzymology; D014316:Tritium
D002352:Carrier Proteins; D011955:Receptors, Drug; C027775:imipramine receptor; D014316:Tritium; D008734:Methoxyhydroxyphenylglycol; D008995:Monoamine Oxidase; D003042:Cocaine; D007099:Imipramine; D009638:Norepinephrine; D004837:Epinephrine