Tubulogenesis in Drosophila: a requirement for the trachealess gene product.
The trachealess (trh) gene of Drosophila is required for embryonic tube formation. In trh mutants, tube-forming cells of the salivary gland, trachea, and filzkörper fail to invaginate to form tubes and remain on the embryo surface. We identified a P-element insertion that disrupts trh function and used the insert to clone and characterize trh. trh is expressed in the salivary duct, trachea, and filzköper primordia, and expression persists in these cells throughout embryogenesis. trh expression in the salivary duct is controlled by the homeotic gene, Sex combs reduced (Scr), and by another salivary gland gene, fork head (fkh). trh is homologous to two transcription factors: the human hypoxia-inducible factor-1 alpha and the Drosophila Single-minded protein.
Isaac
D D
DD
Department of Cell Biology and Anatomy, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205-2196, USA.
Andrew
D J
DJ
D000595:Amino Acid Sequence; D000818:Animals; D030801:Animals, Genetically Modified; D001483:Base Sequence; D051792:Basic Helix-Loop-Helix Proteins; D002490:Central Nervous System / Q000196:embryology / Q000648:ultrastructure; D017630:Connexins; D004251:DNA Transposable Elements; D004268:DNA-Binding Proteins / Q000737:chemistry; D004330:Drosophila / Q000196:embryology / Q000235:genetics*; D029721:Drosophila Proteins; D004847:Epithelial Cells; D004848:Epithelium / Q000196:embryology; D051858:Forkhead Transcription Factors; D018507:Gene Expression Regulation, Developmental; D005816:Genetic Complementation Test; D051793:Hypoxia-Inducible Factor 1; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D007301:Insect Hormones / Q000737:chemistry / Q000235:genetics; D008565:Membrane Proteins / Q000737:chemistry; D008870:Microtubules / Q000502:physiology*; D008969:Molecular Sequence Data; D009154:Mutation; D009419:Nerve Tissue Proteins / Q000737:chemistry; D009687:Nuclear Proteins / Q000737:chemistry / Q000235:genetics; D012333:RNA, Messenger; D018987:Salivary Ducts / Q000196:embryology* / Q000648:ultrastructure; D017386:Sequence Homology, Amino Acid; D014132:Trachea / Q000196:embryology / Q000648:ultrastructure; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics
D051792:Basic Helix-Loop-Helix Proteins; D017630:Connexins; D004251:DNA Transposable Elements; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D051858:Forkhead Transcription Factors; D051793:Hypoxia-Inducible Factor 1; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D007301:Insect Hormones; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D009687:Nuclear Proteins; D012333:RNA, Messenger; D014157:Transcription Factors; C059627:Scr protein, Drosophila; C079324:shakB protein, Drosophila; C089449:sim protein, Drosophila; C099995:trh protein, Drosophila