Molecular cloning of equine interleukin-1 alpha and -beta cDNAs.
Equine interleukin-1 alpha (IL-1 alpha) and IL-1 beta were molecularly cloned to establish a basis for research on inflammatory and immune responses in the horse. Equine peripheral blood mononuclear cells (PBMC) were stimulated with lipopolysaccharide (LPS), and cDNA clones of equine IL-1 alpha and IL-1 beta covering the whole coding sequences were isolated from them. These equine IL-1 alpha and IL-1 beta clones contained open reading frames encoding 271 and 269 amino acids, respectively. The deduced amino acid sequence of equine IL-1 alpha showed 71.6% and 60.2% similarity with that of human and murine IL-1 alpha, respectively. Similarly, the amino acid sequence of equine IL-1 beta showed 66.7% and 61.8% similarity with that of human and murine IL-1 beta, respectively. In both equine IL-1 alpha and IL-1 beta, amino acids at miristoylation sites were well conserved. Dot blot analysis indicated that the expression of IL-1 beta was predominant to that of IL-1 alpha in equine PBMC stimulated with LPS or phorbol myristate acetate.
Kato
H
H
Department of Veterinary Internal Medicine, Faculty of Agriculture, University of Tokyo, Japan.
Ohashi
T
T
Nakamura
N
N
Nishimura
Y
Y
Watari
T
T
Goitsuka
R
R
Tsujimoto
H
H
Hasegawa
A
A
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002273:Carcinogens / Q000494:pharmacology; D003001:Cloning, Molecular; D017931:DNA Primers / Q000737:chemistry; D018076:DNA, Complementary / Q000032:analysis* / Q000737:chemistry; D006736:Horses / Q000235:genetics / Q000276:immunology*; D006801:Humans; D015151:Immunoblotting / Q000662:veterinary; D007375:Interleukin-1 / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D016133:Polymerase Chain Reaction / Q000662:veterinary; D017386:Sequence Homology, Amino Acid; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D002273:Carcinogens; D017931:DNA Primers; D018076:DNA, Complementary; D007375:Interleukin-1; D008070:Lipopolysaccharides; D013755:Tetradecanoylphorbol Acetate