Immunohistochemical detection of epidermal growth factor in glycol methacrylate embedded tissue.
This study addresses a variety of immunohistochemical conditions for detecting EGF in 3.5% paraformaldehyde fixed, glycol methacrylate embedded tissue including antigen unmasking with trypsin, dilution of primary antibody, and incubation time with primary antibody. Color development was achieved with a biotinylated secondary antibody linked to an avidin biotinylated peroxidase complex. Trypsinization and a 12 hr incubation with the primary antibody was essential to detect EGF in this system. Adequate staining could be achieved with a 1:100 dilution of the primary antibody.
Ogborn
M R
MR
Department of Pediatrics and Child Health, University of Manitoba, Winnipeg, Canada.
Sareen
S
S
D000818:Animals; D004815:Epidermal Growth Factor / Q000032:analysis*; D007150:Immunohistochemistry; D008297:Male; D008689:Methacrylates; D051379:Mice; D016613:Plastic Embedding / Q000379:methods*; D013194:Staining and Labeling / Q000379:methods; D013363:Submandibular Gland / Q000033:anatomy & histology / Q000737:chemistry; D014357:Trypsin
D008689:Methacrylates; D004815:Epidermal Growth Factor; C005044:hydroxyethyl methacrylate; D014357:Trypsin