title: Stimulation of endocrine, but not exocrine, pancreatic secretion during 2-deoxy-D-glucose-induced neuroglycopenia in the conscious pig.
abstract: The effects of autonomic nervous activation, initiated by 2-deoxy-D-glucose (2-DG)-induced neuroglycopenia, or endocrine and exocrine pancreatic secretion were investigated in the conscious pig. Pigs were surgically fitted with permanent pancreatic duct and duodenal reentrant cannulas, allowing long-term sampling of pancreatic juice, and a jugular vein catheter for blood sampling and infusion of 2-DG. 2-DG was administered as a 5-min intravenous infusion at three dose levels to conscious pigs. 2-DG (400 mg/kg) was found to elevate plasma glucagon and insulin levels (p < 0.01). In contrast, exocrine pancreatic secretion, measured as volume, total protein output, and output of trypsin activity was not affected by 2-DG at the dose levels of 75, 200, and 400 mg/kg. Secretin (440 pmol/kg/h), however, stimulated pancreatic exocrine output of fluid (p < 0.01), protein (p < 0.01), and trypsin (p < 0.05). It is concluded that autonomic nervous activation by 2-DG-induced neuroglycopenia, in the conscious pig under basal conditions, elevates the plasma levels of glucagon and insulin but does not affect exocrine pancreatic secretion. 2-DG-induced neuroglycopenia is, thus, a suitable model for studying autonomic neural influences on the porcine endocrine pancreas.
authors.affiliation: Department of Medicine, Malmö General Hospital, Lund University, Sweden.
authors.forename: S
authors.initials: S
authors.lastname: Karlsson
authors.forename: S G
authors.initials: SG
authors.lastname: Pierzynowski
authors.forename: B R
authors.initials: BR
authors.lastname: Weström
authors.forename: M J
authors.initials: MJ
authors.lastname: Thaela
authors.forename: B
authors.initials: B
authors.lastname: Ahrén
authors.forename: B W
authors.initials: BW
authors.lastname: Karlsson
mesh_terms: D000818:Animals; D001341:Autonomic Nervous System / Q000187:drug effects; D001786:Blood Glucose / Q000378:metabolism; D003847:Deoxyglucose / Q000494:pharmacology; D005934:Glucagon / Q000097:blood*; D007262:Infusions, Intravenous; D007328:Insulin / Q000097:blood*; D007515:Islets of Langerhans / Q000187:drug effects / Q000294:innervation / Q000378:metabolism*; D010179:Pancreas / Q000187:drug effects / Q000294:innervation / Q000378:metabolism*; D011863:Radioimmunoassay; D012633:Secretin / Q000008:administration & dosage; D013552:Swine
chemical_list: D001786:Blood Glucose; D007328:Insulin; D012633:Secretin; D005934:Glucagon; D003847:Deoxyglucose