Bovine growth hormone induces oscillations in cytosolic free Ca2+ in single rat hepatocytes.
Single rat hepatocytes microinjected with the photoprotein aequorin generate oscillations in the cytosolic free Ca2+ concentration ([Ca2+]i) when stimulated with agonists acting through the phosphoinositide signalling pathway. We show here that, in single rat hepatocytes, bovine growth hormone (bGH) is able to induce [Ca2+]i oscillations which display similarities with oscillations induced by phenylephrine. Thus the rate of rise of intracellular Ca2+ in each oscillation closely resembles that induced by Ins(1,4,5)P3-mediated agonists. However, the duration of bGH-induced oscillations increases with agonist concentration, in contrast to phenylephrine-induced oscillations, which undergo an increase in frequency as the agonist concentration is raised, without any increase in the duration of individual oscillations.
Marrero
I
I
Department of Human Anatomy and Cell Biology, University of Liverpool, UK.
Green
A K
AK
Cobbold
P H
PH
Dixon
C J
CJ
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002417:Cattle; D013006:Growth Hormone / Q000494:pharmacology*; D008099:Liver / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D010656:Phenylephrine / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar
D010656:Phenylephrine; D013006:Growth Hormone; D002118:Calcium