Evolution of the sperm aster after microinjection of isolated human sperm centrosomes into meiotically mature human oocytes.
This study examined the feasibility of isolating and transferring the centrosome-containing region of spermatozoa to mature human oocytes. The findings demonstrate that individual sperm centrosomes can be transferred and are capable of nucleating maternal tubulin to form a well-developed sperm aster in the recipient oocyte. The results are discussed with respect to centrosome function in early human development and applications in clinical in-vitro fertilization in the treatment of certain forms of male factor infertility.
Van Blerkom
J
J
Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder 80309, USA.
Davis
P
P
D002458:Cell Fractionation; D016922:Cellular Senescence / Q000502:physiology; D018385:Centrosome; D005240:Feasibility Studies; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D008297:Male; D008540:Meiosis / Q000502:physiology*; D008845:Microinjections; D008870:Microtubules / Q000648:ultrastructure; D009865:Oocytes / Q000166:cytology*; D013084:Sperm-Ovum Interactions / Q000502:physiology*; D013094:Spermatozoa / Q000648:ultrastructure*; D014404:Tubulin
D014404:Tubulin