Evaluation of ractopamine cross-reactivity in several commercially available beta-agonist enzyme immunoassay kits.
Several enzyme immunoassay test kits are commercially available for screening of matrices including cattle feed, tissues, faeces and urine for the presence of beta-agonists. The kits utilized for this evaluation offer sensitivity as low as 0.1 ng ml-1 (assay solution concentration, not test sample). Evaluation of ractopamine hydrochloride (LY31537) solutions at concentrations as high as 1000 ng ml-1, using six different kits from four different manufacturers, showed cross-reactivity of less than 0.5%.
Wicker
A L
AL
Lilly Research Laboratories, A Division of Eli Lilly and Company, Greenfield, IN 46140-0708, USA.
Turberg
M P
MP
Coleman
M R
MR
D000318:Adrenergic beta-Agonists / Q000032:analysis* / Q000276:immunology; D003429:Cross Reactions; D005069:Evaluation Studies as Topic; D007124:Immunoenzyme Techniques; D010627:Phenethylamines / Q000032:analysis* / Q000276:immunology; D011933:Reagent Kits, Diagnostic
D000318:Adrenergic beta-Agonists; D010627:Phenethylamines; D011933:Reagent Kits, Diagnostic; C060897:ractopamine