Enhanced susceptibility to human immunodeficiency virus infection in CD4+ T lymphocytes genetically deficient in CD43.
CD43 is a cell surface sialoglycoprotein expressed by most cells of hematopoietic origin, including all T lymphocytes. Elimination of CD43 expression by gene targeting in the CEM T cell line results in its increased homotypic adhesion and binding to HIV-1 gp120. Here we report that the CD43-negative CEM cells show increased susceptibility to HIV-1 infection and increased viral replication compared with the parental CD43+ CEM cell line. Increased HIV-1 replication also was observed in CEM cells with diminished CD43 expression secondary to functional inactivation of a single CD43 allele. The CD43- CEM cells were more susceptible to HIV-1-induced cytopathicity than their CD43+ counterparts. HIV-1 replication also was increased in the CD43- CEM cells after transfection with the infectious HIV molecular clone pNL4-3. These data suggest that factors that diminish CD43 expression on T lymphocytes may enhance their susceptibility to HIV-1 infection.
Srinivas
R V
RV
Department of Infectious Diseases, St. Jude Children's Research Hospital, Memphis, Tennessee 38105, USA.
Su
T
T
Trimble
L A
LA
Lieberman
J
J
Ardman
B
B
D015703:Antigens, CD / Q000235:genetics / Q000502:physiology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology* / Q000821:virology*; D002460:Cell Line; D002470:Cell Survival; D003588:Cytopathogenic Effect, Viral / Q000276:immunology; D018390:Gene Targeting; D015658:HIV Infections / Q000209:etiology* / Q000276:immunology; D015497:HIV-1 / Q000472:pathogenicity* / Q000502:physiology; D006801:Humans; D007700:Kinetics; D051917:Leukosialin; D012795:Sialoglycoproteins / Q000172:deficiency* / Q000235:genetics / Q000276:immunology; D014779:Virus Replication
D015703:Antigens, CD; D051917:Leukosialin; C465014:SPN protein, human; D012795:Sialoglycoproteins