Induction of the adipose differentiation-related protein in liver of etomoxir-treated rats.
The effects of etomoxir, an irreversible carnitine palmitoyltransferase I inhibitor, on the liver protein pattern and on liver morphology were examined by two-dimensional gel electrophoresis in female Sprague-Dawley rats treated with 125 mg/kg/day etomoxir for 28 days. In livers of treated animals a protein spot was found which was not present in controls. The spot was identified by internal amino acid sequence analysis as the adipose differentiation-related protein (ADRP). The expression of ADRP in liver is a novel finding as the protein has been described previously as adipocyte-specific. Additionally we found histopathologic evidence of lipid accumulation in the livers of etomoxir rats. The data show that for each treated rat there was a good correlation between ADRP levels and degree of lipid droplet formation. This observation may suggest a potential relationship between drug-induced expression of ADRP in liver and lipid accumulation.
Steiner
S
S
Sandoz Pharma Ltd, Basel, Switzerland. steiner@dstox.pharma.sandoz.ch.
Wahl
D
D
Mangold
B L
BL
Robison
R
R
Raymackers
J
J
Meheus
L
L
Anderson
N L
NL
Cordier
A
A
D000595:Amino Acid Sequence; D000818:Animals; D002334:Carnitine O-Palmitoyltransferase / Q000037:antagonists & inhibitors; D015180:Electrophoresis, Gel, Two-Dimensional; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D004852:Epoxy Compounds / Q000494:pharmacology*; D005260:Female; D008099:Liver / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D008565:Membrane Proteins / Q000378:metabolism*; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry; D000070780:Perilipin-2; D051381:Rats; D017207:Rats, Sprague-Dawley
D004791:Enzyme Inhibitors; D004852:Epoxy Compounds; D008565:Membrane Proteins; D010455:Peptides; D000070780:Perilipin-2; C000602984:Plin2 protein, rat; D002334:Carnitine O-Palmitoyltransferase; C054207:etomoxir