Epipolarization microscopic immunogold assay: a combination of immunogold silver staining, enzyme-linked-immunosorbent assay and epipolarization microscopy.
We describe a new immunoassay which combines an immunosorbent assay, immunogold silver staining and epipolarization microscopy. Our new assay procedure features multiple samples on a single microscope slide, and high sensitivity of epipolarization microscope for detection of silver-enhanced colloidal gold as a final immunoassay product. We call the new immunoassay "on slide immunogold assay" (OSIGA). This new method uses biotinylated antibody and streptavidin-gold reaction with silver enhancement technique. With OSIGA it is possible to investigate 30 samples on a single microscopic slide. Our preliminary studies used 10-20 microliter samples and detected nanogram quantities of a standardized protein solution. Unlike enzyme linked immunosorbent assay (ELISA), which has a limited time for reading the final color products, the OSIGA specimens can be dried or resin mounted for longer storage and future reference.
Gao
K
K
Department of Cell Biology, Neurobiology and Anatomy, University of Cincinnati College of Medicine, Ohio 45267, USA.
Morris
R E
RE
Wei
C
C
D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006041:Goats; D007150:Immunohistochemistry / Q000379:methods*; D008859:Microscopy, Polarization / Q000379:methods*; D011817:Rabbits; D016622:Silver Staining / Q000379:methods*