Inhibition of 1,2-dimethylhydrazine-induced oxidative DNA damage by green tea extract in rat.
Following subcutaneous injection of 1,2-dimethylhydrazine (DMH), which is carcinogenic to rat colon and liver, to Sprague-Dawley rats, a significant increase of 8-hydroxydeoxyguanosine (8-OHdG) was observed in the DNA of colonic mucosa and liver. The 8-OHdG formation reached the maximal level at about 24 h after the DMII injection. On the other hand, no increase of 8-OHdG was observed in the DNA of the kidney. Drinking green tea extract (GTE) for ten days prior to the DMH injection significantly inhibited the formation of 8-OHdG in the colon. These findings demonstrate that DMH causes oxidative damage to the DNA of its target organ, and that GTE protects colonic mucosa from this oxidative damage.
Inagake
M
M
First Department of Surgery, Kyoto Prefectural University of Medicine.
Yamane
T
T
Kitao
Y
Y
Oya
K
K
Matsumoto
H
H
Kikuoka
N
N
Nakatani
H
H
Takahashi
T
T
Nishimura
H
H
Iwashima
A
A
D019813:1,2-Dimethylhydrazine; D000080242:8-Hydroxy-2'-Deoxyguanosine; D000284:Administration, Oral; D000818:Animals; D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D001397:Azoxymethane / Q000037:antagonists & inhibitors / Q000633:toxicity; D001711:Biotransformation; D002392:Catechin / Q000494:pharmacology; D003106:Colon / Q000737:chemistry / Q000187:drug effects*; D004247:DNA / Q000187:drug effects*; D004249:DNA Damage; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000032:analysis; D003979:Diazonium Compounds / Q000378:metabolism / Q000633:toxicity; D004127:Dimethylhydrazines / Q000008:administration & dosage / Q000037:antagonists & inhibitors* / Q000493:pharmacokinetics / Q000633:toxicity; D016166:Free Radical Scavengers; D007279:Injections, Subcutaneous; D007413:Intestinal Mucosa / Q000737:chemistry / Q000187:drug effects; D007668:Kidney / Q000737:chemistry / Q000187:drug effects*; D008099:Liver / Q000737:chemistry / Q000187:drug effects*; D008297:Male; D008745:Methylation / Q000187:drug effects; D008746:Methylazoxymethanol Acetate / Q000031:analogs & derivatives / Q000378:metabolism; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013662:Tea / Q000737:chemistry*
D016588:Anticarcinogenic Agents; D003979:Diazonium Compounds; D004127:Dimethylhydrazines; D016166:Free Radical Scavengers; D010936:Plant Extracts; D013662:Tea; D008746:Methylazoxymethanol Acetate; D000080242:8-Hydroxy-2'-Deoxyguanosine; D002392:Catechin; D004247:DNA; D003849:Deoxyguanosine; D019813:1,2-Dimethylhydrazine; C030667:methylazoxymethanol; D001397:Azoxymethane