title: Microdialysis as a tool to assess interstitial norepinephrine levels in adipose tissue of spontaneously hypertensive rats.
abstract: The microdialysis technique was applied to the study of norepinephrine (NE) metabolism in white adipose tissue of spontaneously hypertensive (SHR, n = 6) and normotensive Wistar-Kyoto (WKY, n = 6) rats. Mean concentrations of interstitial NE were much higher in SHR as compared to WKY (mean +/- SEM: 980.9 +/- 125.6 pg/ml vs 520.7 +/- 96.1 pg/ml; p = 0.01) over the 180 min experimental period. These results are consistent with the hypothesis that sustained outflow from nerve endings of the peripheral sympathetic system may play a role in the maintenance of arterial hypertension. Owing to its low invasiveness, the microdialysis technique allows to continuously monitor NE extracellular levels in conscious and freely-moving animals.
authors.affiliation: Institute of Clinical Medicine and Nephrology, University of Parma Medical School, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Bergamaschi
authors.forename: A
authors.initials: A
authors.lastname: Cabassi
authors.forename: A
authors.initials: A
authors.lastname: Mutti
authors.forename: I
authors.initials: I
authors.lastname: Franchini
authors.forename: A
authors.initials: A
authors.lastname: Borghetti
mesh_terms: D000273:Adipose Tissue / Q000737:chemistry / Q000378:metabolism*; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D006973:Hypertension / Q000378:metabolism*; D008297:Male; D017551:Microdialysis / Q000295:instrumentation / Q000379:methods*; D009638:Norepinephrine / Q000032:analysis / Q000378:metabolism*; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D013997:Time Factors
chemical_list: D009638:Norepinephrine