A hypochlorite-fast metachromatic quick stain for human megakaryocytes.
Megakaryocytes in aspirates of normal and abnormal human bone marrow displayed rose-pink metachromasia when stained with a violet polymethine dye. The color of the megakaryocytes persisted when the stained sample was exposed to a dilute solution of sodium hypochlorite. Under the same conditions, other hematopoietic cells became colorless. This stain may be useful for demonstrating megakaryocyte from both healthy and diseased bone marrow specimens.
Kass
L
L
Department of Pathology, Case Western Reserve University School of Medicine, Cleveland, Ohio 44109, USA.
D001854:Bone Marrow Cells; D004396:Coloring Agents; D006801:Humans; D007211:Indoles; D008533:Megakaryocytes / Q000166:cytology* / Q000187:drug effects; D012973:Sodium Hypochlorite / Q000494:pharmacology*; D013194:Staining and Labeling / Q000379:methods*; D013921:Thrombocytopenia / Q000473:pathology
D004396:Coloring Agents; D007211:Indoles; C098209:VPM chloride; D012973:Sodium Hypochlorite