A recombinant fowlpox virus expressing the envelope antigen of subgroup A avian leukosis/sarcoma virus.
A recombinant fowlpox virus (FPV) was constructed by inserting cloned sequences from Schmidt-Ruppin subgroup A avian sarcoma virus coding for the viral envelope (env) antigen into a nonessential region of FPV DNA downstream from a synthetic promoter. Sera from chickens hyperimmunized with the recombinant FPV neutralized the infectivity of the homologous subgroup A virus (RCASBP/AP) but only weakly neutralized the infectivity of Rous sarcoma virus, another subgroup A avian leukosis virus. Similarly, vaccination of 1-day-old chicks with this recombinant FPV protected against infection with RCASBP/AP virus but not against infection with another subgroup A Rous-associated virus (RAV-1). These results show that such a recombinant FPV can be used to protect chickens against avian leukosis virus and confirm previous observations that a type-specific antigenic variability existed within the subgroup A avian leukosis/sarcoma virus group.
Nazerian
K
K
USDA, Avian Disease and Oncology Laboratory, East Lansing, Michigan 48823, USA.
Yanagida
N
N
D001355:Alpharetrovirus / Q000235:genetics*; D000818:Animals; D000956:Antigens, Viral / Q000235:genetics*; D001353:Avian Leukosis / Q000276:immunology / Q000517:prevention & control; D001483:Base Sequence; D002478:Cells, Cultured; D002645:Chickens; D005586:Fowlpox / Q000276:immunology / Q000517:prevention & control; D005587:Fowlpox virus / Q000235:genetics* / Q000276:immunology*; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D001357:Sarcoma, Avian / Q000276:immunology / Q000517:prevention & control; D014759:Viral Envelope Proteins / Q000235:genetics* / Q000276:immunology*
D000956:Antigens, Viral; D014759:Viral Envelope Proteins