Molecular genetic analysis of the 17p11.2 region in patients with hereditary neuropathy with liability to pressure palsies (HNPP).
Hereditary neuropathy with liability to pressure palsies (HNPP) is in most cases associated with an interstitial deletion of the same 1.5-Mb region at 17p11.2 that is duplicated in Charcot-Marie-Tooth type 1A (CMT1A) patients. Unequal crossing-over following misalignment at flanking repeat sequences (CMT1A-REP), either leads to tandem duplication in CMT1A patients or deletion in HNPP patients. With the use of polymorphic DNA markers located within the CMT1A/HNPP duplication/deletion region we detected the HNPP deletion in 16 unrelated HNPP patients, 11 of Belgian and 5 of French origin. In all cases, the 1.5-Mb size of the HNPP deletion was confirmed by EcoRI dosage analysis using a CMT1A-REP probe. In the 16 HNPP patients, the same 370/320-kb EagI deletion-junction fragments were detected with pulsed field gel electrophoresis (PFGE), while in CMT1A patients, a 150-kb EagI duplication-junction fragment was seen. Thus, PFGE analysis of EagI-digested DNA with a CMT1A-REP probe allows direct detection of the HNPP deletion or the CMT1A duplication for DNA diagnostic purposes.
Timmerman
V
V
Born Bunge Foundation, University of Antwerp (UIA), Department of Biochemistry, Belgium.
Löfgren
A
A
Le Guern
E
E
Liang
P
P
De Jonghe
P
P
Martin
J J
JJ
Verhalle
D
D
Robberecht
W
W
Gouider
R
R
Brice
A
A
Van Broeckhoven
C
C
D015139:Blotting, Southern; D002607:Charcot-Marie-Tooth Disease / Q000235:genetics; D002874:Chromosome Mapping; D002886:Chromosomes, Human, Pair 17; D003434:Crossing Over, Genetic; D004247:DNA / Q000235:genetics; D003711:Demyelinating Diseases / Q000235:genetics*; D005260:Female; D006801:Humans; D008297:Male; D010375:Pedigree; D010523:Peripheral Nervous System Diseases / Q000235:genetics*; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping; D017384:Sequence Deletion
D004247:DNA