title: Permeability of human isolated airways increases after hydrogen peroxide and poly-L-arginine.
abstract: In asthma, the airway epithelium may be damaged by oxidants and cationic proteins produced by eosinophils. The degree of epithelial damage correlates with the level of bronchial hyperreactivity. An increase in airway permeability to bronchoactive agonists may be one of the factors responsible for airway hyperresponsiveness in asthma. We investigated the effects of the oxidant hydrogen peroxide and the major basic protein (MPB) analogue poly-L-arginine on the permeability and morphology of nonasthmatic human peripheral airways. In addition, we examined whether airway permeability depends on airway size. Human airway tubes (generation 7 to 12) mounted in an organ bath were luminally perfused with Krebs-Henseleit buffer containing the hydrophilic tracer 111In-diethyletriamine pentaacetic acid (111In-DTPA) or the lipophilic tracer 14C-antipyrine (14C-AP). Permeability of the airways was calculated from the fluxes of the tracer molecules across the airway wall. After experiments, light- and electromicroscopic examination of the airway epithelium was performed. Baseline permeability to 14C-AP was five times greater than to 111In-DTPA. Luminal exposure of the airways to 100 mmol/L H2O2 produced a significant, sixfold increase in permeability to 111In-DTPA but not to 14C-AP, indicating the opening of paracellular pathways. The H2O2-induced increase in permeability was partly reversible. Luminal exposure to polyarginine for 3 h and 16 h produced a significant 4.5- and 7-fold increase in permeability to 111In-DTPA, respectively. Histologic examination of epithelium exposed to H2O2 of poly-L-arginine showed focal loss of superficial cells with preservation of basal cells. Baseline airway permeability increased with decreasing airway size (rs = -0.75, p < 0.01). These results suggest that epithelial damage due to eosinophil products may increase airway permeability, and that this may at least partly explain the increased responsiveness to inhaled stimuli in asthma.
authors.affiliation: Department of Pediatrics, Erasmus University, Rotterdam, The Netherlands.
authors.forename: A R
authors.initials: AR
authors.lastname: Hulsmann
authors.forename: H R
authors.initials: HR
authors.lastname: Raatgeep
authors.forename: J C
authors.initials: JC
authors.lastname: den Hollander
authors.forename: W H
authors.initials: WH
authors.lastname: Bakker
authors.forename: P R
authors.initials: PR
authors.lastname: Saxena
authors.forename: J C
authors.initials: JC
authors.lastname: de Jongste
mesh_terms: D000328:Adult; D000368:Aged; D000983:Antipyrine / Q000493:pharmacokinetics; D001798:Blood Proteins / Q000494:pharmacology; D001980:Bronchi / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D002250:Carbon Radioisotopes; D047091:Eosinophil Granule Proteins; D004848:Epithelium / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D005260:Female; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D066298:In Vitro Techniques; D007205:Indium Radioisotopes; D018836:Inflammation Mediators / Q000494:pharmacology*; D008297:Male; D008875:Middle Aged; D016877:Oxidants / Q000494:pharmacology*; D004369:Pentetic Acid / Q000493:pharmacokinetics; D010455:Peptides / Q000494:pharmacology*; D010539:Permeability; D012260:Ribonucleases
chemical_list: D001798:Blood Proteins; D002250:Carbon Radioisotopes; D047091:Eosinophil Granule Proteins; D007205:Indium Radioisotopes; D018836:Inflammation Mediators; D016877:Oxidants; D010455:Peptides; C015462:polyarginine; D004369:Pentetic Acid; D006861:Hydrogen Peroxide; D012260:Ribonucleases; D000983:Antipyrine