Study of the stability of tylosin A in aqueous solutions.
The decomposition of the 16-membered ring macrolide antibiotic tylosin A in aqueous buffers has been investigated in the pH range 2-13, by means of a liquid chromatographic assay with ultraviolet detection at 280 nm. In acidic medium, tylosin A is converted into tylosin B, while in neutral and alkaline medium, tylosin A aldol is formed together with a number of polar decomposition products of unknown identity. The decomposition kinetics have been studied as a function of the type and concentration of the buffer, ionic strength, pH and temperature.
Paesen
J
J
Katholieke Universiteit Leuven, Laboratorium voor Farmaceutische Chemie en Analyse van Geneesmiddelen, Faculteit Farmaceutische Wetenschappen, Belgium.
Cypers
W
W
Pauwels
K
K
Roets
E
E
Hoogmartens
J
J
D000900:Anti-Bacterial Agents / Q000737:chemistry*; D002021:Buffers; D002853:Chromatography, Liquid; D004355:Drug Stability; D006863:Hydrogen-Ion Concentration; D009994:Osmolar Concentration; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature; D015645:Tylosin / Q000737:chemistry*
D000900:Anti-Bacterial Agents; D002021:Buffers; D015645:Tylosin