Aminopyrine infusion breath test for the determination of changes in P450 metabolism in vivo.
1. An osmotic mini-pump was used to maintain a constant infusion of radiolabelled [N-dimethyl-14C] aminopyrine into a rat. After implanting the mini-pump, 14CO2 expiration rate was constant within 12 h, and this rate was maintained for 192 h. 2. Treatment with 2-diethylaminoethyl-2,2-diphenylvalerate HCl (SKF 525-A) or cimetidine, inhibitors of P450-dependent metabolism, resulted in both dose- and time-dependent inhibition of the expiration of 14CO2.
Kuwahara
S K
SK
Department of Pharmacology, University of Chicago, IL 60637, USA.
Shinn
T J
TJ
Schreider
B D
BD
Phan
M L
ML
Kotake
A N
AN
D000632:Aminopyrine; D000818:Animals; D001944:Breath Tests; D002250:Carbon Radioisotopes; D065607:Cytochrome P-450 Enzyme Inhibitors; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
D002250:Carbon Radioisotopes; D065607:Cytochrome P-450 Enzyme Inhibitors; D000632:Aminopyrine; D003577:Cytochrome P-450 Enzyme System