title: Activation of lavage lymphocytes in lung injuries caused by radiotherapy for lung cancer.
abstract: OBJECTIVE
  Radiation pneumonitis sometimes extends beyond the irradiated area of a lung and can also affect the opposite lung. Some immunological mechanisms, in addition to simple direct injury of the lungs by radiation, seem to be involved in the onset of radiation pneumonitis. To clarify such mechanisms, the effects of radiation on local inflammatory cells in lungs, in particular, lymphocytes, were examined.
  
  
  METHODS
  A comparison was made of bronchoalveolar lavage fluid (BALF) findings from 13 irradiated patients (RT group) and 15 nonirradiated patients (non-RT group) with lung cancer. Patients who later developed radiation pneumonitis (RP group) and those who did not (RP-free group) were also compared. Using a two-color flowcytometer, radiation-induced changes in local inflammatory cells in lungs were analyzed. This included analyses of human leukocyte-associated antigen (HLADR) and intercellular adhesion molecule-1 (ICAM-1) expression on T-cells, which are though to be involved in cell activation and interactions between cells.
  
  
  RESULTS
  The following aspects of BALF were higher in the RT group than in the non-RT group: (a) the percentage of lymphocytes and eosinophiles; (b) the incidence of HLADR-positive CD4+T-cells and HLADR-positive CD8+T-cells; and (c) the incidence of ICAM-1--positive T-cells. The following aspects of BALF were higher in the RP group than in the RP-free group: (a) the total cell counts; (b) the percentage of lymphocytes; and (c) the incidence of ICAM-1-positive T-cells. A significant relationship was seen between the incidence of ICAM-1 expression on T-cells and the number of days from the initiation of radiotherapy to the onset of radiation pneumonitis.
  
  
  CONCLUSIONS
  These data suggest that irradiation can induce accumulation of activated T-cells (HLADR and ICAM-1--positive T-cells) in the lung. This accumulation may be closely linked to radiation-induced lung injury. It is also suggested that the incidence of ICAM-1--positive T-cells in BALF may serve as a useful clinical marker of radiation pneumonitis.
authors.affiliation: First Department of Internal Medicine, Osaka Medical College, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Nakayama
authors.forename: S
authors.initials: S
authors.lastname: Makino
authors.forename: Y
authors.initials: Y
authors.lastname: Fukuda
authors.forename: K Y
authors.initials: KY
authors.lastname: Min
authors.forename: A
authors.initials: A
authors.lastname: Shimizu
authors.forename: N
authors.initials: N
authors.lastname: Ohsawa
mesh_terms: D000328:Adult; D000368:Aged; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology*; D015496:CD4-Positive T-Lymphocytes / Q000737:chemistry / Q000528:radiation effects*; D018414:CD8-Positive T-Lymphocytes / Q000737:chemistry / Q000528:radiation effects*; D002289:Carcinoma, Non-Small-Cell Lung / Q000473:pathology / Q000532:radiotherapy*; D018288:Carcinoma, Small Cell / Q000473:pathology / Q000532:radiotherapy*; D005260:Female; D006684:HLA-DR Antigens / Q000032:analysis*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis*; D008175:Lung Neoplasms / Q000473:pathology / Q000532:radiotherapy*; D008213:Lymphocyte Activation; D008297:Male; D008875:Middle Aged; D017564:Radiation Pneumonitis / Q000276:immunology*
chemical_list: D006684:HLA-DR Antigens; D018799:Intercellular Adhesion Molecule-1