Murine and human mast cell express acetylcholinesterase.
Expression of catalytically active protein was detected in a murine mast cell line. The primary type of AChE mRNA produced by these cells was found to be the brain and muscle type by PCR amplification of alternative exons from the 3' of mast cells AChE cDNA. AChE was further found to be expressed in the HMC-1 the human mast cell precursor line. Furthermore, utilizing the single cell RT-PCR method we detected AChE mRNA expression in Fc epsilon RI-positive single cells derived from human colonic mucosal biopsies. Our findings predict the involvement of mast cell AChE in neuronal-mast cell interactions.
Nechushtan
H
H
Department of Biochemistry, Hebrew University-Hadassah Medical School, Hebrew University of Jerusalem, Israel.
Soreq
H
H
Kuperstein
V
V
Tshori
S
S
Razin
E
E
D000110:Acetylcholinesterase / Q000235:genetics / Q000378:metabolism*; D017398:Alternative Splicing; D000818:Animals; D001483:Base Sequence; D002450:Cell Communication / Q000502:physiology; D002460:Cell Line; D003106:Colon / Q000166:cytology / Q000201:enzymology; D017931:DNA Primers / Q000235:genetics; D015870:Gene Expression; D006801:Humans; D066298:In Vitro Techniques; D007413:Intestinal Mucosa / Q000166:cytology / Q000201:enzymology; D008407:Mast Cells / Q000201:enzymology* / Q000502:physiology; D051379:Mice; D008969:Molecular Sequence Data; D009474:Neurons / Q000502:physiology; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014018:Tissue Distribution
D017931:DNA Primers; D012333:RNA, Messenger; D000110:Acetylcholinesterase