title: Cellular and humoral immune responses to recombinant antigens in sheep infected with Toxoplasma gondii.
abstract: Immune responses to recombinant fragments of the Toxoplasma gondii antigens ROP2 and GRA2 were investigated in sheep naturally and experimentally infected with T. gondii oocysts. Specific serum antibodies to C-terminal fragments were detected by ELISA. Cell-mediated responses in peripheral blood mononuclear cells were demonstrated by proliferation and interferon-gamma production following in vitro stimulation with the ROP2 fragment. This data indicates the presence of epitopes for sheep B cells in the recombinant GRA2 fragment and for both B and T cells in the ROP2 fragment.
authors.affiliation: Department of Veterinary Parasitology, Liverpool School of Tropical Medicine, UK.
authors.forename: S N
authors.initials: SN
authors.lastname: Coughlan
authors.forename: E
authors.initials: E
authors.lastname: Saman
authors.forename: D
authors.initials: D
authors.lastname: Jacobs
authors.forename: C
authors.initials: C
authors.lastname: Mercier
authors.forename: M F
authors.initials: MF
authors.lastname: Cesbron-Delauw
authors.forename: A J
authors.initials: AJ
authors.lastname: Trees
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000096:biosynthesis*; D000953:Antigens, Protozoan / Q000276:immunology*; D005260:Female; D007371:Interferon-gamma / Q000096:biosynthesis; D008213:Lymphocyte Activation; D010446:Peptide Fragments / Q000276:immunology; D015800:Protozoan Proteins / Q000276:immunology; D011994:Recombinant Proteins / Q000276:immunology; D012756:Sheep; D012757:Sheep Diseases / Q000276:immunology*; D014122:Toxoplasma / Q000276:immunology*; D014124:Toxoplasmosis, Animal / Q000276:immunology*
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; C106990:Gra2 protein, Toxoplasma gondii; D010446:Peptide Fragments; D015800:Protozoan Proteins; D011994:Recombinant Proteins; D007371:Interferon-gamma