title: Nucleotide sequence analysis of recent epidemic strains of enterovirus 70.
abstract: Nucleotide sequences of the genome of enterovirus 70 (EV70) isolated in Osaka in 1993 were determined, and compared with those of the past epidemic strains. Nucleotide substitution rates in 332 bp, 174 bp, and 178 bp of the genes encoding viral protein (VP)1, VP2, or VP3 were 9.0, 7.5, and 5.6% between Kumoi-2/93 and J670/71, respectively. Likewise, the putative amino acid substitution rates were 1.8, 0, and 0%. It seems that the epidemic strains of EV70 in Japan have been evolving at a constant high nucleotide substitution rate but almost all the substitutions were synonymous.
authors.affiliation: Laboratory of Virology, Osaka Prefectural Institute of Public Health, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Yamazaki
authors.forename: I
authors.initials: I
authors.lastname: Oishi
authors.forename: Y
authors.initials: Y
authors.lastname: Minekawa
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D001483:Base Sequence; D003232:Conjunctivitis, Acute Hemorrhagic / Q000453:epidemiology / Q000821:virology*; D017931:DNA Primers / Q000737:chemistry; D015342:DNA Probes / Q000737:chemistry; D004279:DNA, Viral / Q000032:analysis* / Q000737:chemistry; D004196:Disease Outbreaks; D004770:Enterovirus / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D004769:Enterovirus Infections / Q000453:epidemiology / Q000821:virology*; D005814:Genes, Viral / Q000235:genetics; D006801:Humans; D007564:Japan / Q000453:epidemiology; D008875:Middle Aged; D008969:Molecular Sequence Data; D009154:Mutation; D016133:Polymerase Chain Reaction; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000235:genetics
chemical_list: D017931:DNA Primers; D015342:DNA Probes; D004279:DNA, Viral; D014764:Viral Proteins