The effect of human flt-3 ligand on committed progenitor cell production from normal, aplastic anaemia and Diamond-Blackfan anaemia bone marrow.
We investigated the effect of the human ligand for flt-3 (FL) on the committed progenitor colony formation of normal bone marrow (BM) (n = 9) and BM from four aplastic anaemia (AA) and three Diamond-Blackfan anaemia (DBA) patients. Methylcellulose committed progenitor cell assays were carried out using FL alone and in combinations with granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukin-3 (IL-3) and c-kit ligand (KL). FL alone had a limited, though significant, effect on the production of granulocyte-macrophage colony-forming unit (CFU-GM) colonies from normal BM and showed an additive effect with IL-3 and GM-CSF separately, but not in combination. FL did not increase the stimulation of KL and did not have an effect on the production of erythroid progenitor colonies. FL had no effect on the AA and DBA BMs studied.
Scopes
J
J
Department of Cellular and Molecular Sciences, St George's Hospital Medical School, London.
Daly
S
S
Ball
S E
SE
McGuckin
C P
CP
Gordon-Smith
E C
EC
Gibson
F M
FM
D000328:Adult; D000741:Anemia, Aplastic / Q000473:pathology*; D001853:Bone Marrow / Q000473:pathology*; D005199:Fanconi Anemia / Q000473:pathology*; D005260:Female; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D006801:Humans; D007223:Infant; D007377:Interleukin-3 / Q000494:pharmacology; D008297:Male; D008565:Membrane Proteins / Q000494:pharmacology*; D019009:Proto-Oncogene Proteins c-kit / Q000494:pharmacology
D007377:Interleukin-3; D008565:Membrane Proteins; C084881:flt3 ligand protein; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D019009:Proto-Oncogene Proteins c-kit