Influence of cadmium ions on endothelin-1 binding and calcium signaling in rat glioma C6 cells.
Cadmium ions did not influence the binding of endothelin-1 (ET-1) to its receptor on the surface of rat glioma C6 and rat aorta A10 cells. This was studied (a) by the binding of 1251-ET-1 to intact cells in the absence or presence of cadmium (Cd2+) and (b) by analysis of the receptor/ET-1 complex after crosslinking with disuccinimidyl suberate (DSS) or ethylene glycol-bis-(succinimidyl succinate) (EGS) on SDS PAGE. Using Fura-2 and Quin-2 loaded C6 rat glioma cells, it was shown that Cd2+ ions strongly interfered with the ET-1 induced Ca(2+)-influx in C6 glioma cells (IC50 approximately 10 microM).
Koschel
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K
Institut für Virologie und Immunbiologie, Universität Würzburg, Germany.
Meissner
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NN
Tas
P W
PW
D000818:Animals; D001345:Autoradiography; D002104:Cadmium / Q000633:toxicity*; D002118:Calcium / Q000378:metabolism*; D002460:Cell Line; D004586:Electrophoresis; D016232:Endothelins / Q000378:metabolism* / Q000494:pharmacology; D005456:Fluorescent Dyes; D005910:Glioma; D009131:Muscle, Smooth, Vascular / Q000166:cytology; D011485:Protein Binding / Q000187:drug effects; D051381:Rats; D044022:Receptor, Endothelin A; D017466:Receptors, Endothelin / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects*; D013050:Spectrometry, Fluorescence; D014407:Tumor Cells, Cultured
D016232:Endothelins; D005456:Fluorescent Dyes; D044022:Receptor, Endothelin A; D017466:Receptors, Endothelin; D002104:Cadmium; D002118:Calcium