[Determination of the antiviral activity of a naturally occurring sulfated xylomannan under various experimental conditions].
The effects of different experimental conditions on the antiviral activity of the sulphated xylomannan F6, isolated from the red seaweed Nothogenia fastigiata, against the replication of herpes simplex virus type 1 and 2 (HSV-1 and HSV-2) were studied. The compound was equally effective against several strains of HSV-1 (F, KOS, B-2006 and Field) and HSV-2 (G) in different cell lines (Vero, HEp-2 and BHK-21). Furthermore, the antiviral activity of F6 was independent of the method employed to determine the 50% inhibitory concentration (IC50) (virus yield or plaque reduction tests). The value of the IC50 against HSV-1 was not significantly affected by the initial virus concentration since a variation in the multiplicity of infection from 0.0001 to 0.1 UFP/cell only increased twofold the IC50 from 0.61 to 1.35 micrograms/ml, respectively. The potent inhibitory effect of F6 against high doses of HSV-1 represents a significant advantage for this sulphated polysaccharide with respect to other compounds only active against low viral inocula.
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Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina.
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D000818:Animals; D000998:Antiviral Agents / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D002294:Carcinoma, Squamous Cell / Q000473:pathology; D002460:Cell Line / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002522:Chlorocebus aethiops; D006224:Cricetinae; D004353:Drug Evaluation, Preclinical; D006801:Humans; D007668:Kidney; D008351:Mannans / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D012624:Seaweed / Q000737:chemistry*; D018139:Simplexvirus / Q000187:drug effects / Q000502:physiology; D012878:Skin Neoplasms / Q000473:pathology; D014407:Tumor Cells, Cultured / Q000187:drug effects; D014709:Vero Cells / Q000187:drug effects; D010948:Viral Plaque Assay; D014779:Virus Replication / Q000187:drug effects
D000998:Antiviral Agents; D008351:Mannans
Determinación de la actividad antiviral de un xilomanano sulfatado de origen natural en distintas condiciones experimentales.