title: Galectin-3, a laminin binding protein, fails to modulate adhesion of human melanoma cells to laminin.
abstract: Galectin-3 is a laminin binding protein which expression is altered in a variety of human carcinomas including colon, breast and endometrium. In these tumors, we consistently observed a down regulation of galectin-3 expression related to increased aggressiveness. Galectin-3 belongs to a family of galactose-binding lectins and binds laminin through its numerous poly-N-acetyllactosamine chains. To date, the exact role of galectin-3 in the complex interactions between cancer cells and laminin has not been clearly defined. Adhesion of melanoma cells to laminin is a critical event during tumor invasion and metastasis. In this study, we explore the possibility that galectin-3 could modulate attachment of two human melanoma cell lines to laminin. A2058 and A375 melanoma cell expressed galectin-3 on their surface as demonstrated by immunofluorescence, and attached to laminin in an in vitro assay. We demonstrate that neither recombinant galectin-3 nor an affinity purified antigalectin-3 antiserum altered adhesion of A2058 or A375 melanoma cells to laminin. Our data strongly suggest that galectin-3 is not a key element in adhesion of the melanoma cells to laminin. These results are not surprising in light of the observation that galectin-3 expression is down regulated in cancer and that increased adhesion to laminin is a constant feature of invasive cancer cells.
authors.affiliation: Metastasis Research Laboratory, University of Liège, Belgium.
authors.forename: F A
authors.initials: FA
authors.lastname: van den Brûle
authors.forename: C
authors.initials: C
authors.lastname: Buicu
authors.forename: M E
authors.initials: ME
authors.lastname: Sobel
authors.forename: F T
authors.initials: FT
authors.lastname: Liu
authors.forename: V
authors.initials: V
authors.lastname: Castronovo
mesh_terms: D000943:Antigens, Differentiation / Q000032:analysis / Q000502:physiology*; D002448:Cell Adhesion / Q000187:drug effects; D037502:Galectin 3; D006801:Humans; D007797:Laminin / Q000502:physiology*; D037102:Lectins / Q000502:physiology*; D008545:Melanoma / Q000473:pathology*; D009361:Neoplasm Invasiveness; D011994:Recombinant Proteins / Q000494:pharmacology; D014407:Tumor Cells, Cultured
chemical_list: D000943:Antigens, Differentiation; D037502:Galectin 3; D007797:Laminin; D037102:Lectins; D011994:Recombinant Proteins