FISH and PRINS, a strategy for rapid chromosome screening: application to the assessment of aneuploidy in human sperm.
The co-utilization of FISH and PRINS techniques for in situ chromosome screening was tested on human sperm nuclei. We used a centromeric repeat probe specific for chromosome 4 for FISH. PRINS reactions were performed with alpha-satellite primers specific for either chromosomes 9 or chromosome 18. Double labeling was obtained and estimates of disomy rates were carried out for the three chromosomes.
Pellestor
F
F
CNRS UPR 9008, St. Charles Hospital, Montpellier, France.
Quenesson
I
I
Coignet
L
L
Girardet
A
A
Andréo
B
B
Lefort
G
G
Charlieu
J P
JP
D000328:Adult; D000782:Aneuploidy; D001483:Base Sequence; D002869:Chromosome Aberrations / Q000175:diagnosis; D025063:Chromosome Disorders; D002874:Chromosome Mapping; D017931:DNA Primers / Q000737:chemistry; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008297:Male; D008856:Microscopy, Fluorescence / Q000379:methods*; D008969:Molecular Sequence Data; D013094:Spermatozoa / Q000648:ultrastructure*
D017931:DNA Primers