title: Establishment of immortal hepatocytes from a CYP3A7-transgenic/p53-knockout mouse.
abstract: Immortal hepatocytes were established from a CYP3A7-transgenic/p53-knockout mouse. The cells could be maintained with shorter doubling time after culture for 1 month. Detectable amounts of CYP3A7 mRNA and the activity of 7-propoxycoumarin O-depropylase, one of the representative CYP3A activities, were present in the immortal hepatocytes. Albumin mRNA, which is specifically expressed in the liver, remained in these cells.
authors.affiliation: Division of Drug Metabolism, Faculty of Pharmaceutical Sciences, Hokkaido University, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Shimoji
authors.forename: K
authors.initials: K
authors.lastname: Hattori
authors.forename: S
authors.initials: S
authors.lastname: Itoh
authors.forename: K
authors.initials: K
authors.lastname: Nakayama
authors.forename: M
authors.initials: M
authors.lastname: Katsuki
authors.forename: S
authors.initials: S
authors.lastname: Aizawa
authors.forename: T
authors.initials: T
authors.lastname: Yokoi
authors.forename: T
authors.initials: T
authors.lastname: Kamataki
mesh_terms: D015660:7-Alkoxycoumarin O-Dealkylase / Q000235:genetics*; D000818:Animals; D002478:Cells, Cultured; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D016158:Genes, p53; D006801:Humans; D008099:Liver / Q000166:cytology* / Q000201:enzymology; D051379:Mice; D018345:Mice, Knockout; D008822:Mice, Transgenic; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D012333:RNA, Messenger; D003577:Cytochrome P-450 Enzyme System; D015660:7-Alkoxycoumarin O-Dealkylase