[Proto-oncogene point mutation of c-Ha-ras in bronchogenic carcinoma].
11 bronchogenic carcinoma and 8 corresponding paracarcinoma specimens were detected for activating point mutation in codon 12 of c-Ha-ras gene by Southern blotting hybridization technique. The c-Ha-ras gene was found to be activated by point mutation in codon 12 in 8 of bronchogenic carcinoma and 1 of paracarcinoma tissues. The mutative and unmutative hybrid band was both discovered in 2 of paracarcinoma tissues. Analysis of clinical and pathological features, we conclude that: (1) The point mutation of c-Ha-ras gene codon 12 is found in 77.8% bronchogenic carcinoma among Chinese; (2) At least in part cases, mutation of c-Ha-ras gene codon 12 is not the "initiation" event, but may be a early-stage event in the pathogenesis; (3) No apparent association between the c-Ha-ras gene codon 12 mutation and pathological types, disease stages and history of smoking is present.
Wang
L
L
PUMC Hospital, CAMS, Beijing.
Li
L
L
Zhu
Y
Y
Fang
F
F
Zuo
J
J
D000230:Adenocarcinoma / Q000235:genetics; D002283:Carcinoma, Bronchogenic / Q000235:genetics*; D002294:Carcinoma, Squamous Cell / Q000235:genetics; D003062:Codon; D011905:Genes, ras; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics*; D017354:Point Mutation; D000090063:Proto-Oncogene Mas
D003062:Codon; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas