Interleukin 10 release during endotoxaemia in chimpanzees: role of platelet-activating factor and interleukin 6.
Interleukin (IL-)10 has been demonstrated to inhibit endotoxin-induced production of a number of pro-inflammatory cytokines. The present study sought to compare the appearances in the circulation of IL-10, IL-6 and IL-8, and to assess the roles of endogenously produced platelet-activating factor (PAF) and IL-6 in IL-10 release during endotoxaemia in chimpanzees. Intravenous injection of endotoxin (lot EC-5, 4 ng/kg, n = 8) induced a transient rise in serum IL-10 concentrations, peaking after 2 h (213 +/- 70 pg/ml; P < 0.05). No correlations existed between peak IL-10 levels, and peak IL-6 and IL-8 levels. Neither infusion of the specific PAF antagonist TCV-309 (n = 4), nor infusion of a neutralizing anti-IL-6 monoclonal antibody (n = 4) influenced endotoxin-induced IL-10 release. IL-10 release elicited by injection of endotoxin is not mediated by PAF or IL6.
van der Poll
T
T
Department of Internal Medicine, Academic Medical Center, University of Amsterdam, The Netherlands.
Jansen
J
J
Levi
M
M
ten Cate
H
H
Hack
C E
CE
Aarden
L A
LA
ten Cate
J W
JW
van Deventer
S J
SJ
D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage; D004731:Endotoxins; D004926:Escherichia coli; D007275:Injections, Intravenous; D016753:Interleukin-10 / Q000096:biosynthesis*; D015850:Interleukin-6 / Q000096:biosynthesis / Q000502:physiology*; D016209:Interleukin-8 / Q000096:biosynthesis; D007546:Isoquinolines / Q000494:pharmacology; D002679:Pan troglodytes; D010972:Platelet Activating Factor / Q000502:physiology*; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology; D011726:Pyridinium Compounds / Q000494:pharmacology; D044005:Tetrahydroisoquinolines; D014115:Toxemia / Q000139:chemically induced / Q000276:immunology*
D000911:Antibodies, Monoclonal; D004731:Endotoxins; D015850:Interleukin-6; D016209:Interleukin-8; D007546:Isoquinolines; D010972:Platelet Activating Factor; D010975:Platelet Aggregation Inhibitors; D011726:Pyridinium Compounds; D044005:Tetrahydroisoquinolines; D016753:Interleukin-10; C067430:TCV 309