title: Nonisotopic hybridization assay for determination of relative amounts of genotypic human immunodeficiency virus type 1 zidovudine resistance.
abstract: A nonisotopic hybridization assay for human immunodeficiency virus genotypic zidovudine resistance determination is described. Biotinylated PCR product was hybridized with enzyme-labeled probes for wild-type or resistant mutant sequences and detected colorimetrically or chemiluminescently in a microplate format. Changes in mutant-to-wild-type ratios allow for the monitoring of longitudinal patient samples.
authors.affiliation: Chiron Corporation, Emeryville, California 94608, USA.
authors.forename: P S
authors.initials: PS
authors.lastname: Eastman
authors.forename: E
authors.initials: E
authors.lastname: Boyer
authors.forename: L
authors.initials: L
authors.lastname: Mole
authors.forename: J
authors.initials: J
authors.lastname: Kolberg
authors.forename: M
authors.initials: M
authors.lastname: Urdea
authors.forename: M
authors.initials: M
authors.lastname: Holodniy
mesh_terms: D000998:Antiviral Agents / Q000494:pharmacology*; D004352:Drug Resistance, Microbial / Q000235:genetics; D005838:Genotype; D015658:HIV Infections / Q000097:blood; D015497:HIV-1 / Q000187:drug effects* / Q000235:genetics; D006801:Humans; D008163:Luminescent Measurements; D009154:Mutation; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D012194:RNA-Directed DNA Polymerase / Q000235:genetics*; D018894:Reverse Transcriptase Inhibitors / Q000494:pharmacology*; D012680:Sensitivity and Specificity; D015215:Zidovudine / Q000494:pharmacology*
chemical_list: D000998:Antiviral Agents; D018894:Reverse Transcriptase Inhibitors; D015215:Zidovudine; D012194:RNA-Directed DNA Polymerase