Cholinotoxic effects of beta-amyloid (1-42) peptide on cortical projections of the rat nucleus basalis magnocellularis.
Beta-amyloid(1-42) peptide (betaAP) was injected into the right nucleus basalis magnocellularis (nbm) of rats. After a 14-day survival time, the acetylcholinesterase and choline acetyltransferase activities and the number of muscarinic receptors were found biochemically to be significantly reduced in the ipsilateral frontal cortices. Confirmation of these data with silver staining also revealed degeneration of the projective fibers of the nbm to the frontal cortex. These results demonstrate the cholinotoxicity of betaAP in an in vivo animal model.
Harkany
T
T
Department of Zoology and Cell Biology, Attila József University, Szeged, Hungary.
Lengyel
Z
Z
Soós
K
K
Penke
B
B
Luiten
P G
PG
Gulya
K
K
D000110:Acetylcholinesterase / Q000187:drug effects; D016564:Amyloid beta-Protein Precursor / Q000494:pharmacology*; D000818:Animals; D001667:Binding, Competitive; D002795:Choline O-Acetyltransferase / Q000187:drug effects*; D005625:Frontal Lobe / Q000187:drug effects*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013377:Substantia Innominata / Q000187:drug effects*; D013997:Time Factors
D016564:Amyloid beta-Protein Precursor; D002795:Choline O-Acetyltransferase; D000110:Acetylcholinesterase