Purification of exo- and endoinulinase from crude inulinase extract for the analysis of fructans.
This paper describes a technique for rapid and easy separation of a crude inulinase extract into exo- and endoinulinase from a commercially available inulinase stock (Novozym 230 from Novo Nordisk A/S) by means of non-denaturating polyacrylamide gel electrophoresis. The purified enzymes were tested with inulin. Characterization of the reaction products was done by high-performance thin-layer chromatography, size exclusion chromatography and semi-preparative reverse-phase high-performance liquid chromatography.
Baumgartner
S
S
Institute of Chemistry, Universität für Bodenkultur, Vienna, Austria.
Praznik
W
W
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002855:Chromatography, Thin Layer; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D005630:Fructans / Q000032:analysis* / Q000378:metabolism*; D006026:Glycoside Hydrolases / Q000302:isolation & purification* / Q000378:metabolism; D007527:Isoenzymes / Q000302:isolation & purification / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism
D005630:Fructans; D007527:Isoenzymes; D011134:Polysaccharides; D006026:Glycoside Hydrolases; C023086:inulinase