Specific expression in brain and liver driven by the hemopexin promoter in transgenic mice.
Transgenic mice harboring the human hemopexin promoter sequences linked to the lacZ reporter gene were generated and analyzed for temporal and spatial distribution of beta-galactosidase. Upstream sequences spanning from -1800, -700 and -500 bp to the transcription start point direct regulated beta-galactosidase expression specifically to the liver and to the brain of transgenic mice. These results suggest that the 500 bp DNA fragment flanking the 5'end of the human hemopexin gene contains the cis-acting elements required for tissue and developmental stage-specific expression in vivo and provide evidence for a new extrahepatic site of expression of the hemopexin gene.
Tolosano
E
E
Department of Genetics, Biology and Medical Chemistry, University of Turin, Italy.
Cutufia
M A
MA
Hirsch
E
E
Silengo
L
L
Altruda
F
F
D000210:Acute-Phase Reaction; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000378:metabolism; D017931:DNA Primers / Q000737:chemistry; D015870:Gene Expression; D006466:Hemopexin / Q000235:genetics*; D006801:Humans; D017403:In Situ Hybridization; D008099:Liver / Q000196:embryology / Q000378:metabolism*; D051379:Mice; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D009132:Muscles / Q000378:metabolism; D009409:Nerve Crush; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics; D012584:Sciatic Nerve; D014425:Turpentine
D017931:DNA Primers; D012333:RNA, Messenger; D006466:Hemopexin; D014425:Turpentine