The expression of ornithine decarboxylase antizyme mRNA and protein in rat motoneurons.
The distribution of ornithine decarboxylase antizyme messenger ribonucleic acid (AZ mRNA) and AZ-like immunoreactivity (LI) was studied in the brainstem and spinal cord motoneurons and in the extraocular and triceps surae muscles of rat. In situ hybridization showed AZ mRNA in the gray matter of the spinal cord at different levels of spinal cord with highest AZ mRNA levels in the ventral horn of the spinal cord. No apparent changes in AZ mRNA contents were seen after unilateral transection of the sciatic nerve in lumbar motoneurons. AZ-immunoreactive (IR) motoneurons were observed in the nucleus of the VI cranial nerve and in the ventral horn of the spinal cord. These motoneurons also showed ornithine decarboxylase (ODC)-LI. Subcellularly, AZ-LI was observed both in the nuclei and cytoplasm of labeled motoneurons. Heavily stained AZ-IR nerve fibers and myoneural junctions were observed among muscle fibers in different muscles. In addition, the nuclei of muscle fibers showed AZ-LI.
Junttila
T
T
Department of Anatomy, University of Tampere, Medical School, Finland.
Rechardt
L
L
Hietala
O A
OA
Pelto-Huikko
M
M
D000818:Animals; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D009046:Motor Neurons / Q000378:metabolism*; D065108:Ornithine Decarboxylase Inhibitors; D011506:Proteins / Q000235:genetics* / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
D065108:Ornithine Decarboxylase Inhibitors; D011506:Proteins; D012333:RNA, Messenger; C031017:ornithine decarboxylase antizyme