Premature expression of cyclin B sensitizes human HT1080 cells to caffeine-induced premature mitosis.
Eukaryotic cells do not normally initiate mitosis when DNA replication is blocked. This cell cycle checkpoint can be bypassed in some cells, however, by treatment with caffeine and certain other chemicals. Although S-phase arrested hamster cells undergo mitosis-specific events such as premature chromosome condensation (PCC) and nuclear envelope disassembly when exposed to caffeine, human cells show little response under the same conditions. To further investigate the molecular basis of this cell type specificity, a panel of hamster/human whole cell hybrids was created. The frequency of caffeine-induced PCC and the level of cyclin-B-associated H1 kinase activity in the various hybrids were directly correlated with the extent of cyclin B synthesis during S-phase arrest. To determine whether expression of cyclin B alone could sensitize human cells to caffeine, cyclin B1 was transiently overexpressed in S-phase arrested HT1080 cells. The transfected cell population displayed a 5-fold increase in the frequency of caffeine-induced PCC when compared with normal HT1080 cells, roughly equivalent to the frequency of cells expressing exogenous epitope-tagged cyclin B1. In addition, immunofluorescent microscopy showed that individual cells overexpressing cyclin B1 during S phase arrest underwent PCC when exposed to caffeine. These results provide direct evidence that premature expression of cyclin B1 can make cells more vulnerable to chemically-induced uncoupling of mitosis from the completion of DNA replication.
Tam
S W
SW
Department of Molecular and Cellular Toxicology, Harvard School of Public Health, Boston, Massachusetts 02115, USA.
Belinsky
G S
GS
Schlegel
R
R
D000818:Animals; D016466:CHO Cells; D002110:Caffeine / Q000494:pharmacology*; D002459:Cell Fusion; D002875:Chromosomes / Q000648:ultrastructure; D006224:Cricetinae; D016213:Cyclins / Q000096:biosynthesis / Q000235:genetics*; D005354:Fibrosarcoma; D015870:Gene Expression; D006801:Humans; D006822:Hybrid Cells; D008938:Mitosis / Q000187:drug effects*; D006656:Protamine Kinase / Q000378:metabolism; D016196:S Phase; D013997:Time Factors; D014162:Transfection; D014407:Tumor Cells, Cultured
D016213:Cyclins; D002110:Caffeine; D006656:Protamine Kinase