Phrenic afferent input excites C1-C2 spinal neurons in rats.
Effects of electrical stimulation of the ipsilateral phrenic nerve above the heart were determined on cells in the C1-C2 spinal cord segments of 27 rats anesthetized with pentobarbital. Forty-five cells that responded to this stimulus were included in this study. These cells then were examined at the same stimulus parameters for effects of stimulating the ipsilateral phrenic nerve below the heart, the contralateral phrenic nerve above the heart, and/or the left (ipsilateral) cervical vagus nerve. Ipsilateral phrenic nerve stimulation below the heart had no effect on 20 of 20 cells tested. Seven of 16 cells tested for effects of contralateral phrenic nerve stimulation above the heart were excited and activity of 9 cells was unaffected. Activity changes and activation latencies were not significantly different in the 7 cells excited by both ipsilateral and contralateral phrenic nerve stimulation. Thirty-seven of 45 cells excited by ipsilateral phrenic nerve stimulation also were excited by stimulation of the left cervical (ipsilateral) vagus nerve. The mean increase in cell activity was significantly greater following vagal nerve stimulation compared to phrenic nerve stimulation, and mean activation latency was significantly longer for vagal stimulation. Excitatory receptive somatic fields were classified for 35 cells. Somatic field locations for most cells (80%) included the ipsilateral neck and jaw. Activity of 26 cells was increased by both noxious pinch and brushing the hair, activity of 6 cells was increased only by noxious pinch, and activity of 3 cells was increased only by brushing the hair. Results of this study indicate that there are afferent fibers in the phrenic nerve above the heart, but not below the heart, that excite cells in the C1-C2 segments of the spinal cord. Most cells also were excited by noxious stimuli applied to their somatic receptive fields. Thus, the phrenic nerve may provide a pathway for referral of pain to the neck and jaw from thoracic structures.
Razook
Joel C
JC
Department of Physiology, University of Oklahoma Health Sciences Center, P.O. Box 26901, Oklahoma City, OK 73190 USA.
Chandler
Margaret J
MJ
Foreman
Robert D
RD
D000344:Afferent Pathways / Q000502:physiology; D000818:Animals; D004558:Electric Stimulation; D008297:Male; D009333:Neck; D009474:Neurons / Q000502:physiology*; D010146:Pain / Q000503:physiopathology*; D010496:Pericardium / Q000294:innervation*; D010791:Phrenic Nerve / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000166:cytology / Q000502:physiology*; D014630:Vagus Nerve / Q000502:physiology*