title: Opposing effects of ultraviolet B irradiation on interleukin-1 receptor antagonist and interleukin-1 alpha messenger RNA expression in a human epidermoid carcinoma cell line.
abstract: Interleukin-1 receptor antagonist (IL-1RA) is a cytokine that acts to antagonize IL-1 activity without agonist function. The expression of IL-1RA has been reported in many cell types, including the keratinocyte that covers the outer most part of the skin. However the modulation of IL-1RA by ultraviolet B (UVB), which is the most biologically active UV, has not been reported yet. We therefore selected a keratinocyte cell line with a cytokine-producing profile similar to that of keratinocytes and tested the effect of UVB on its ability to produce IL-1RA mRNA. IL-1RA mRNA was constitutively expressed in the cell line and began to be suppressed by 3 h after the UVB irradiation with 100 mJ/cm2. The level of IL-1RA expression became lowest by 16 h after the irradiation with 100 mJ/cm2. Simultaneously, IL-1 alpha mRNA started to increase by 1 h and peaked by 3-16 h after the irradiation with 10-100 mJ/cm2. The differential expression of IL-1 alpha and IL-1RA mRNA following exposure to a high dose (100 mJ/cm2) of UVB may markedly potentiate the role of IL-1 in UV-induced inflammation.
authors.affiliation: Department of Dermatology, Yonsei University College of Medicine, Seoul, Republic of Korea.
authors.forename: W
authors.initials: W
authors.lastname: Lew
authors.forename: S H
authors.initials: SH
authors.lastname: Lee
authors.forename: Y K
authors.initials: YK
authors.lastname: Park
mesh_terms: D015139:Blotting, Southern; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000276:immunology*; D002460:Cell Line; D017931:DNA Primers; D015342:DNA Probes; D015972:Gene Expression Regulation, Neoplastic / Q000528:radiation effects*; D006801:Humans; D053590:Interleukin 1 Receptor Antagonist Protein; D007375:Interleukin-1 / Q000032:analysis / Q000235:genetics* / Q000528:radiation effects*; D007624:KB Cells / Q000276:immunology / Q000378:metabolism / Q000528:radiation effects; D015603:Keratinocytes / Q000378:metabolism / Q000528:radiation effects; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis / Q000528:radiation effects*; D011994:Recombinant Proteins / Q000235:genetics* / Q000528:radiation effects*; D012795:Sialoglycoproteins / Q000235:genetics* / Q000528:radiation effects*; D012867:Skin / Q000378:metabolism / Q000528:radiation effects; D013997:Time Factors; D014466:Ultraviolet Rays / Q000145:classification
chemical_list: D017931:DNA Primers; D015342:DNA Probes; C507763:IL1RN protein, human; D053590:Interleukin 1 Receptor Antagonist Protein; D007375:Interleukin-1; D012333:RNA, Messenger; D011994:Recombinant Proteins; D012795:Sialoglycoproteins