Physical map of the HLA-A/HLA-F subregion and identification of two new coding sequences.
As part of an effort to characterize the hemochromatosis gene, we selected three non-chimeric yeast artificial chromosomes (YACs) overlapping with the YAC B30 previously described and forming an 800 kilobase contig covering the HLA-A/HLA-F region. The precise physical map of these YACs and of the corresponding genomic region were established. Nine concentrated sites of CpG cutter elements, potentially HTF islands, were mapped. In addition, several probes have been generated as tools for mapping and examining transcripts produced in the region. This allowed for the characterization and localization of two new coding sequences, provisionally named HCG (for hemochromatosis candidate gene) and numbered VIII and IX.
Pichon
L
L
Department of Biochemistry and Molecular Biology, UPR 41 CNRS - "Recombinaisons génétiques" Faculté de Médecine, 2 avenue du Professeur Léon Bernard, 35043 Rennes Cedex, France.
Giffon
T
T
Chauvel
B
B
Carn
G
G
Bouric
P
P
El Kahloun
A
A
Legall
J Y
JY
David
V
V
D015152:Blotting, Northern; D018244:Chromosomes, Artificial, Yeast; D002896:Chromosomes, Human, Pair 6; D003001:Cloning, Molecular; D016172:DNA Fingerprinting; D018076:DNA, Complementary / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D015723:Gene Library; D006680:HLA Antigens / Q000235:genetics; D015234:HLA-A Antigens / Q000235:genetics; D006432:Hemochromatosis / Q000235:genetics*; D015395:Histocompatibility Antigens Class I / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D014158:Transcription, Genetic; D011544:Pseudogenes
D018076:DNA, Complementary; D006680:HLA Antigens; D015234:HLA-A Antigens; D015395:Histocompatibility Antigens Class I; C062341:HLA-F antigens; C483818:POLR1HASP protein, human