Cytogenetic, molecular and phenotypic characterization of the newly established renal carcinoma cell line KJ29. Evidence of translocations for chromosomes 1 and 3.
The established non papillary human renal carcinoma cell line (RCC) KJ29 was submitted to a multiparametric characterization to evaluate its potential use for in vitro and in vivo studies. The cell line grows in vitro as monolayer as well as cell suspension. Cytogenetic analysis has shown a modal chromosome number of 50 with some marker chromosomes, including rearrangements of chromosomes 1 and 3. The antigenic phenotype is characterized by co-expression of cytokeratin and vimentin, as well as expression of urothelium differentiation antigens, low levels of class II MHC antigens and no class I antigens. A differential expression of the VLA-3 integrin heterodimer has been detected between the adherent and non adherent cell population. The cell line which is highly tumorigenic in athymic mice displays expression of erb B-2 and c-met oncogenes and high expression of cell-cycle related and Ha-ras 1 genes.
Barletta
C
C
Laboratory of Immunology, Regina Elena Cancer Institute, Rome, Italy.
Bartolazzi
A
A
Cimino Reale
G
G
Gambari
R
R
Nastruzzi
C
C
Barbieri
R
R
Del Senno
L
L
Castagnoli
A
A
Natali
P G
PG
D000818:Animals; D002292:Carcinoma, Renal Cell / Q000235:genetics* / Q000276:immunology; D002878:Chromosomes, Human, Pair 1; D002893:Chromosomes, Human, Pair 3; D005260:Female; D016259:Genes, myc; D011905:Genes, ras; D006801:Humans; D007680:Kidney Neoplasms / Q000235:genetics* / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008875:Middle Aged; D014178:Translocation, Genetic; D014407:Tumor Cells, Cultured