title: Establishment and characterization of a villous lymphoma cell line from splenic B-cell lymphoma.
abstract: A new B-cell line (VL51) with cytoplasmic villi was established from a female patient with splenic lymphoma with circulating villous lymphocytes (SLVL). The patient exhibited a clinical picture characteristic of SLVL, including massive enlargement of the spleen. Tartrate-resistant acid phosphatase (TRAP)-negative villous lymphocytes were seen in the peripheral blood, bone marrow (BM) and both red and white pulps of the spleen. Monoclonality of the VL51 cell line was confirmed by clonal genotype abnormalities in the immunoglobulin heavy chain (IgH) gene and the T-cell receptor beta (TCR beta) gene. Evidence for commitment of phenotype of the VL51 cell line to the B lineage was also shown by the immunophenotype, including expression of CD10, CD19, CD20 and surface immunoglobins. The VL51 cells were positive for Epstein-Barr virus nuclear antigen (EBNA). The VL51 cell line is the first SLVL cell line to be established, and it is expected to be useful in clarifying the leukemogenesis of SLVL.
authors.affiliation: Third Department of Internal Medicine, Nippon Medical School, Tokyo, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Inokuchi
authors.forename: J
authors.initials: J
authors.lastname: Abo
authors.forename: H
authors.initials: H
authors.lastname: Takahashi
authors.forename: K
authors.initials: K
authors.lastname: Miyake
authors.forename: S
authors.initials: S
authors.lastname: Inokuchi
authors.forename: K
authors.initials: K
authors.lastname: Dan
authors.forename: T
authors.initials: T
authors.lastname: Nomura
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism; D000368:Aged; D001402:B-Lymphocytes / Q000201:enzymology / Q000648:ultrastructure; D005260:Female; D015326:Gene Rearrangement, B-Lymphocyte, Heavy Chain; D015333:Gene Rearrangement, beta-Chain T-Cell Antigen Receptor; D006651:Histocytochemistry; D006801:Humans; D016130:Immunophenotyping; D007527:Isoenzymes / Q000378:metabolism; D007621:Karyotyping; D016393:Lymphoma, B-Cell / Q000201:enzymology / Q000235:genetics / Q000473:pathology*; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D008871:Microvilli / Q000648:ultrastructure; D013160:Splenic Neoplasms / Q000201:enzymology / Q000235:genetics / Q000473:pathology*; D000071681:Tartrate-Resistant Acid Phosphatase; D014407:Tumor Cells, Cultured / Q000201:enzymology / Q000473:pathology
chemical_list: D007527:Isoenzymes; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase