title: Anti-AIDS agents--XIX. Neotripterifordin, a novel anti-HIV principle from Tripterygium wilfordii: isolation and structural elucidation.
abstract: A new kaurane type diterpene lactone, neotripterifordin (1), has been isolated from the roots of Tripterygium wilfordii. The structure of 1 was elucidated by spectroscopic methods, which included the concerted application of a number of 2-D NMR techniques including 1H-1H COSY, phase-sensitive NOESY, HETCOR, and long-range HETCOR. Compound 1 showed potent anti-HIV replication activity in H9 lymphocyte cells with an EC50 of 25 nM and TI of 125.
authors.affiliation: Natural Products Laboratory, School of Pharmacy, University of North Carolina, Chapel Hill 27599, USA.
authors.forename: K
authors.initials: K
authors.lastname: Chen
authors.forename: Q
authors.initials: Q
authors.lastname: Shi
authors.forename: T
authors.initials: T
authors.lastname: Fujioka
authors.forename: T
authors.initials: T
authors.lastname: Nakano
authors.forename: C Q
authors.initials: CQ
authors.lastname: Hu
authors.forename: J Q
authors.initials: JQ
authors.lastname: Jin
authors.forename: R E
authors.initials: RE
authors.lastname: Kilkuskie
authors.forename: K H
authors.initials: KH
authors.lastname: Lee
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000302:isolation & purification* / Q000494:pharmacology*; D002417:Cattle; D002478:Cells, Cultured; D002681:China; D004224:Diterpenes / Q000302:isolation & purification* / Q000494:pharmacology; D015497:HIV-1 / Q000187:drug effects*; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects / Q000821:virology; D013058:Mass Spectrometry; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D018517:Plant Roots / Q000737:chemistry; D010947:Plants, Toxic / Q000737:chemistry*; D013055:Spectrophotometry, Infrared; D014779:Virus Replication / Q000187:drug effects
chemical_list: D000998:Antiviral Agents; D004224:Diterpenes; D010936:Plant Extracts; C097941:neotripterifordin