Ethanol-induced single-strand DNA breaks in rat brain cells.
Male Sprague-Dawley rats were intubated with 4 g/kg body weight of ethanol (in a 20%, v/v, water solution). Brain cells were analyzed for single-strand DNA breaks at various post-ethanol administration time points using an alkaline microgel electrophoresis assay. Results showed a significant increase in single-strand DNA breaks in brain cells that peaked at approx. 4 h and returned to control level within 6 h after ethanol administration.
Singh
N P
NP
Department of Psychiatry & Behavioral Sciences, School of Medicine, University of Washington, Seattle 98195-6560, USA. narendra@u. washington.edu
Lai
H
H
Khan
A
A
D000284:Administration, Oral; D000704:Analysis of Variance; D000818:Animals; D001921:Brain / Q000166:cytology / Q000187:drug effects*; D004249:DNA Damage; D004277:DNA, Single-Stranded / Q000032:analysis / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004587:Electrophoresis, Agar Gel / Q000379:methods; D000431:Ethanol / Q000008:administration & dosage / Q000633:toxicity*; D008297:Male; D009153:Mutagens / Q000633:toxicity*; D051381:Rats; D017207:Rats, Sprague-Dawley
D004277:DNA, Single-Stranded; D009153:Mutagens; D000431:Ethanol