title: Assessment of circulating blood volume in calves with a total artificial heart.
abstract: Measurement of the circulating blood volume (CBV) is essential to a proper understanding of the hemodynamic performance of total artificial hearts (TAHs). Recently, the authors employed CBV measurements using indocyanine green dye in calves with a TAH. The advantages of this method over previous methods using radionuclides include simplicity, low cost, and the capability of repeated and frequent measurements. Reproducibility of the measurements was demonstrated in three normal calves with a relative standard deviation of 3.9 +/- 2.4%. CBV was measured in eight calves with the Cleveland Clinic-Nimbus TAH and compared with that of seven calves that underwent mitral valve replacement. Small standard deviations in pre operative values in both TAH and mitral valve replacement groups demonstrated the precision of CBV measurements. Although there was no change in CBV in the mitral valve replacement group, CBV in the TAH group increased to more than twice the pre operative value after 2 weeks. Although the right atrial pressure increased similarly after TAH implantation, there was no correlation (r = 0.08) between the right atrial pressure and CBV, which suggested a possible inaccuracy in estimating CBV from the right atrial pressure. A negative correlation between the hematocrit value and CBV suggested that hemodilution might be one of the causes of anemia observed in our TAH animals.
authors.affiliation: Department of Biomedical Engineering, Cleveland Clinic Foundation, Ohio 44195, USA.
authors.forename: K
authors.initials: K
authors.lastname: Fukamachi
authors.forename: M E
authors.initials: ME
authors.lastname: Benavides
authors.forename: K E
authors.initials: KE
authors.lastname: Wika
authors.forename: J A
authors.initials: JA
authors.lastname: Manos
authors.forename: A L
authors.initials: AL
authors.lastname: Massiello
authors.forename: H
authors.initials: H
authors.lastname: Harasaki
mesh_terms: D000818:Animals; D001705:Bioprosthesis; D001794:Blood Pressure; D001810:Blood Volume; D002417:Cattle; D004396:Coloring Agents; D005069:Evaluation Studies as Topic; D006325:Heart Atria; D006350:Heart Valve Prosthesis; D006354:Heart, Artificial; D006400:Hematocrit; D007208:Indocyanine Green; D008297:Male; D008943:Mitral Valve / Q000601:surgery; D015203:Reproducibility of Results
chemical_list: D004396:Coloring Agents; D007208:Indocyanine Green