title: Structural evaluation of human and sheep bone and comparison with synthetic hydroxyapatite by FT-Raman spectroscopy.
abstract: The composition of whole human and sheep cortical bone tissue, and of a synthetic hydroxyapatite (P120), were compared using Fourier transform Raman (FT-Raman) spectroscopy. Deproteination procedures to remove the bulk of the collagen present in bone tissue allowed isolation of the mineral phase. A comparison of the spectra obtained from both whole and deproteinated bone with those of synthetic hydroxyapatite showed direct correlation only in the region of 952 cm-1 (symmetric P-O mode). In contrast, human and sheep bone were very closely matched in both, the organic and inorganic structures. The results demonstrate that deproteination of bone is not a necessary precursor to obtain spectral information.
authors.affiliation: Interdisciplinary Research Centre in Biomedical Materials, Queen Mary and Westfield College, University of London, United Kingdom.
authors.forename: I
authors.initials: I
authors.lastname: Rehman
authors.forename: R
authors.initials: R
authors.lastname: Smith
authors.forename: L L
authors.initials: LL
authors.lastname: Hench
authors.forename: W
authors.initials: W
authors.lastname: Bonfield
mesh_terms: D000818:Animals; D018775:Bone Demineralization Technique; D001842:Bone and Bones / Q000737:chemistry*; D002118:Calcium / Q000032:analysis; D002130:Calcium Phosphates / Q000737:chemistry; D003094:Collagen / Q000032:analysis; D005583:Fourier Analysis; D006801:Humans; D006882:Hydroxyapatites / Q000737:chemistry*; D008297:Male; D008875:Middle Aged; D012756:Sheep; D013059:Spectrum Analysis, Raman
chemical_list: D002130:Calcium Phosphates; D006882:Hydroxyapatites; D003094:Collagen; D002118:Calcium