title: The tellurite-resistance determinants tehAtehB and klaAklaBtelB have different biochemical requirements.
abstract: The tehAtehB operon from the Escherichia coli chromosome (32.3 min) mediates resistance to potassium tellurite (K2TeO3) when expressed on a multicopy plasmid such as pUC8 (pTWT100). An MIC of 128 micrograms ml-1 is observed when tehAtehB is expressed in a wild-type host and grown on rich media. In this study, the tehAtehB determinant was transformed into mutants deficient in electron transport processes and/or thiol redox coupling within E. coli. These mutants included ubi, nad, cys, nar, trx, grx, gsh and sod. MICs of tehAtehB transformed into these mutants ranged from 1-16 micrograms K2TeO3 ml-1 compared to 0.03-2 micrograms ml-1 for strains transformed with a control plasmid. The tellurite-resistance determinant locus kilA cloned from the IncP alpha plasmid RK2Ter (pDT1558) was also investigated in these strains. This tellurite-resistance determinant showed little or no dependency on the host genotype. The ability of tehAtehB to mediate resistance in wild-type hosts is limited to rich medium. Rich medium may provide a key unidentified cofactor required by TehATehB that is not provided under minimal conditions. Again, the ability of the kilA determinant to mediate tellurite resistance was independent of medium conditions. These data suggest that either a reducing environment or electron-reducing equivalents are required for tehAtehB to mediate high levels of resistance to potassium tellurite. Therefore, the two resistance determinants studied here possess two very different biochemical mechanisms of resistance. Our data also suggest a mechanism for endogenous resistance to tellurite which involves nitrate reductase, superoxide dismutase, and thiol redox processes.
authors.affiliation: Dept of Medical Microbiology and Immunology, University of Alberta, Edmonton, Canada.
authors.forename: R J
authors.initials: RJ
authors.lastname: Turner
authors.forename: J H
authors.initials: JH
authors.lastname: Weiner
authors.forename: D E
authors.initials: DE
authors.lastname: Taylor
mesh_terms: D003470:Culture Media; D003545:Cysteine / Q000096:biosynthesis; D004352:Drug Resistance, Microbial / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics* / Q000378:metabolism; D005798:Genes, Bacterial; D009536:Niacinamide / Q000096:biosynthesis; D050901:Nitrate Reductase; D009565:Nitrate Reductases / Q000378:metabolism; D009876:Operon; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D050862:Oxidoreductases Acting on Sulfur Group Donors / Q000378:metabolism; D010957:Plasmids / Q000235:genetics; D013438:Sulfhydryl Compounds / Q000378:metabolism; D013691:Tellurium / Q000494:pharmacology*; D014451:Ubiquinone / Q000096:biosynthesis
chemical_list: D003470:Culture Media; D013438:Sulfhydryl Compounds; D014451:Ubiquinone; D009536:Niacinamide; D009565:Nitrate Reductases; D050901:Nitrate Reductase; D050862:Oxidoreductases Acting on Sulfur Group Donors; C026660:tellurous acid; D003545:Cysteine; D013691:Tellurium