Protein characterization and targeted disruption of Grg, a mouse gene related to the groucho transcript of the Drosophila Enhancer of split complex.
The Grg gene encodes a 197 amino acid protein homologous to the amino-terminal domain of the product of the groucho gene of the Drosophila Enhancer of split complex. Analysis with a polyclonal antisera specific for the Grg protein revealed that Grg is a 25 kd nuclear protein that can participate in specific protein-protein interactions. A null mutation of the Grg gene was constructed by gene targeting. Mice homozygous for this mutation completed embryogenesis and were born, but exhibited varying degrees of post-natal growth deficiency. No dosage-sensitive genetic interaction was detected between the Notch1 and Grg genes in mice heterozygous for a Notch1 mutant allele and homozygous for the Grg null mutation.
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Roche Institute of Molecular Biology, Roche Research Center, Nutley, New Jersey, USA.
Gendron-Maguire
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Harbison
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Gridley
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D000483:Alleles; D000818:Animals; D001483:Base Sequence; D051792:Basic Helix-Loop-Helix Proteins; D056970:Co-Repressor Proteins; D004268:DNA-Binding Proteins / Q000235:genetics*; D004330:Drosophila / Q000235:genetics; D029721:Drosophila Proteins; D005314:Embryonic and Fetal Development / Q000235:genetics; D018628:Gene Dosage; D018507:Gene Expression Regulation, Developmental / Q000502:physiology; D005804:Genes, Lethal / Q000502:physiology; D018095:Germ-Line Mutation / Q000502:physiology; D006720:Homozygote; D007301:Insect Hormones / Q000235:genetics*; D051379:Mice; D008817:Mice, Mutant Strains; D008969:Molecular Sequence Data; D011485:Protein Binding / Q000502:physiology; D011506:Proteins / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis; D012097:Repressor Proteins / Q000235:genetics*; D014157:Transcription Factors; D014158:Transcription, Genetic / Q000235:genetics
D051792:Basic Helix-Loop-Helix Proteins; D056970:Co-Repressor Proteins; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D007301:Insect Hormones; D011506:Proteins; D012333:RNA, Messenger; D012097:Repressor Proteins; D014157:Transcription Factors; C084530:E(spl)mdelta-HLH protein, Drosophila; C089462:Tle5 protein, mouse; C091023:gro protein, Drosophila