Central glucopenia induced by 2-deoxy-D-glucose stimulates somatostatin secretion in the rat.
The mechanisms involved in 2-deoxy-D-glucose (2-DG)-induced growth hormone (GH) suppression in the rat were examined. Conscious male rats were given 2-DG by intracerebroventricular (icv) injection and the pulsatile GH secretion was monitored for 6 h. The single icv injection of 2-DG (8 mg/rat) eliminated pulsatile GH secretion in conscious rats. Pretreatment with somatostatin (SS) antiserum completely restored the suppressed GH secretion in the 2-DG treated rats. Hypothalamic GH-releasing hormone (GRH) and SS mRNA levels were not altered by single and multiple icv injections of 2-DG. These findings suggest that 2-DG-induced GH suppression is primarily due to hypersecretion of SS without a significant change at the transcription level in the rat.
Sato
M
M
First Department of Internal Medicine, Kagawa Medical School, Miki-Cho, Japan.
Murao
K
K
Matsubara
S
S
Niimi
M
M
Chihara
K
K
Takahara
J
J
D000818:Animals; D003847:Deoxyglucose / Q000008:administration & dosage / Q000494:pharmacology*; D005947:Glucose / Q000172:deficiency*; D013006:Growth Hormone / Q000378:metabolism; D013007:Growth Hormone-Releasing Hormone / Q000235:genetics; D007031:Hypothalamus / Q000378:metabolism; D007116:Immunization, Passive; D007276:Injections, Intraventricular; D008297:Male; D010507:Periodicity; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013004:Somatostatin / Q000235:genetics / Q000276:immunology / Q000378:metabolism*
D012333:RNA, Messenger; D013004:Somatostatin; D013006:Growth Hormone; D013007:Growth Hormone-Releasing Hormone; D003847:Deoxyglucose; D005947:Glucose