Analyses of 22S dynein binding to Tetrahymena axonemes lacking outer dynein arms.
Tetrahymena thermophila mutants homozygous for the oad mutation become nonmotile when grown at the restrictive temperature of 39 degrees C. Axonemes isolated from nonmotile oad mutants (oad 39 degrees C axonemes) lack approximately 90% of their outer dynein arms and are deficient in 22S dynein. Here we report that oad 39 degrees C axonemes contain 40% of the 22S dynein heavy chains that wild-type axonemes contain and that oad axonemes do not undergo ATP-induced microtubule sliding in vitro. Wild-type 22S dynein will bind to the outer arm position in oad axonemes and restore ATP-induced microtubule sliding in those axonemes. Unlike wild-type 22S dynein, oad 22S dynein does not bind to the outer arm position in oad axonemes. These data indicate that the oad mutation affects some component of the outer arm dynein itself rather than the outer arm dynein binding site. These data also indicate that oad axonemes can be used to assay outer dynein arm function.
Sullivan
J
J
Department of Zoology, Miami University, Oxford, Ohio 45056, USA.
Ludmann
S A
SA
Hamasaki
T
T
Pennock
D G
DG
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D002465:Cell Movement / Q000235:genetics*; D002923:Cilia / Q000378:metabolism* / Q000648:ultrastructure; D004398:Dyneins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D008870:Microtubules / Q000187:drug effects / Q000502:physiology; D009154:Mutation; D011485:Protein Binding / Q000187:drug effects; D013347:Subcellular Fractions / Q000378:metabolism; D016808:Tetrahymena thermophila / Q000235:genetics* / Q000648:ultrastructure
D000255:Adenosine Triphosphate; D004398:Dyneins