The expression of Na+/K(+)-ATPase beta-subunit cRNA injected into Xenopus oocytes is affected by coinjection with alpha-subunit cRNA.
The cRNA for Torpedo californica Na+/K(+)-ATPase beta-subunit (cRNA beta) was injected into Xenopus oocytes alone or with the cRNA for the Na+/K(+)-ATPase alpha-subunit (cRNA alpha). When cRNA beta was injected alone, the amount of the beta-subunit that accumulated in oocytes increased with increasing amounts of injected cRNA beta. When cRNA beta and cRNA alpha were injected simultaneously, less beta-subunit accumulated than when cRNA beta was injected alone, whereas the Na+/K(+)-ATPase activity increased markedly. The decrease in the accumulation of the beta-subunit was dose-dependent upon the cRNA alpha. The mutant beta-subunit unable to assemble with the alpha-subunit accumulated in oocytes independently of cRNA alpha, suggesting that post-translational control mechanisms may serve to reduce the accumulation of the beta-subunit.
Ueno
S
S
Department of Biology, University of Occupational and Environmental Health, Kitakyushu, Japan.
Takeda
K
K
Noguchi
S
S
Izumi
F
F
Kawamura
M
M
D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D015971:Gene Expression Regulation, Enzymologic; D007267:Injections; D009154:Mutation; D009865:Oocytes; D014176:Protein Biosynthesis; D018075:RNA, Complementary / Q000235:genetics / Q000378:metabolism*; D000254:Sodium-Potassium-Exchanging ATPase / Q000096:biosynthesis* / Q000235:genetics; D014101:Torpedo; D014981:Xenopus
D018075:RNA, Complementary; D000254:Sodium-Potassium-Exchanging ATPase