Intracellular localization of dapsone and rifampicin in skin and nerve of multidrug treated leprosy patients.
Intracellular localization of antileprosy drugs dapsone (DDS) and rifampicin (RFP) was carried out on skin and nerve lesions obtained from multidrug treated, multi (BL-LL)- and pauci (BT-TT) bacillary cases of leprosy using immunocytochemical techniques. Intracellular localization of the above drugs especially in macrophages and Schwann cells was aimed by using rabbit raised anti DDS and RFP polyclonal antibody in an indirect peroxidase assay. Our study records both intra and extracellular staining with anti DDS and RFP antibodies in the skin as well as nerve lesions of MB and PB cases treated with MDT. All the nerves under investigation had moderate to severe pathology and hence free diffusion of the drug could be attributed to the broken barrier. Basal lamina around the Schwann cell did not seem to form a barrier. It was also noted that the drug (metabolite) persisted over a long period of time).
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MP
Foundation for Medical Research, Worli, Bombay.
Shetty
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VP
Antia
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D000818:Animals; D003622:Dapsone / Q000032:analysis* / Q000627:therapeutic use; D006801:Humans; D007124:Immunoenzyme Techniques; D007917:Leprostatic Agents / Q000032:analysis* / Q000627:therapeutic use; D007918:Leprosy / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology; D008264:Macrophages / Q000378:metabolism; D051379:Mice; D009417:Nerve Tissue / Q000378:metabolism*; D011817:Rabbits; D012293:Rifampin / Q000032:analysis* / Q000627:therapeutic use; D012583:Schwann Cells / Q000378:metabolism; D012867:Skin / Q000378:metabolism*
D007917:Leprostatic Agents; D003622:Dapsone; D012293:Rifampin