[Site-specific endonuclease and methylase from thermophilic bacteria of Bacillus species IS4].
The site-specific endonuclease R . BspIS4I and methylase M . BspIS4I have been isolated and purified to functional purity from the thermophilic strain of Bacillus species IS4. R . BspIS4I recognizes sequence [sequence: see text] on the DNA and cleaves it as indicated by the arrows to form single-stranded 4-nucleotide 5'-protruding termini. The enzyme is an isoschizomer of BbvII. M . BspIS4I is related to adenine-specific methylase.
Zelinskaia
N V
NV
Kovalevskaia
N P
NP
Matvienko
N N
NN
Zheleznaia
L A
LA
Matvienko
N I
NI
D001407:Bacillus / Q000201:enzymology*; D001483:Base Sequence; D002852:Chromatography, Ion Exchange; D004269:DNA, Bacterial / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D008969:Molecular Sequence Data; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific) / Q000302:isolation & purification* / Q000378:metabolism
D004269:DNA, Bacterial; C097868:DNA modification methylase BspIS4I; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific)
Saĭt-spetsificheskie éndonukleaza i metilaza iz termofil'noĭ bakterii Bacillus species IS4.