A study of topoisomerase activity in human testicular cancers.
Topoisomerases are widely detected in rapidly proliferating cancer cells. Many anti-topoisomerase agents are utilized for cancer chemotherapy. Testicular cancers are highly chemotherapy sensitive, however, 10% of them are refractory to the standard regimen. To investigate the topoisomerase activities in human testicular neoplasms, we examined the activity of topoisomerase I (TopoI) and topoisomerase II (TopoII) in 29 testicular tumors. TopoI activity was observed irrespective of pathological types of the tumor (21/29). TopoII was detected in seminoma and teratocarcinoma (5/29). In our experience, seminoma was relatively sensitive to chemotherapy including anti-TopoII agents. Our results suggest that Topol inhibitors could be more effective against seminoma as well as the other types of testicular tumors.
Sano
K
K
Department of Urology, School of Medicine, Yokohama City University, Japan.
Shuhin
T
T
D015153:Blotting, Western; D004264:DNA Topoisomerases, Type I / Q000378:metabolism*; D004250:DNA Topoisomerases, Type II / Q000378:metabolism*; D018237:Germinoma / Q000201:enzymology; D006801:Humans; D008297:Male; D013736:Testicular Neoplasms / Q000201:enzymology*
D004264:DNA Topoisomerases, Type I; D004250:DNA Topoisomerases, Type II