Enhancement of nitric oxide production from activated macrophages by a purified form of ginsenoside (Rg1).
We studied the actions of purified ginsenosides Rg1 and Rb1 on nitric oxide production from macrophages and a macrophage cell line RAW264-7. Although neither Rg1 nor Rb1 induced nitric oxide from resting macrophages, Rg1 enhanced the production of nitric oxide from IFN-gamma activated-macrophages or RAW cells. Rg1 also enhanced the production of nitric oxide from macrophages cocultured with nonadherent spleen cells stimulated by conA, LPS or anti-CD3. Rb1, however, did not significantly enhance nitric oxide production from stimulated macrophages or RAW cells. Rg1 enhanced the tumor cell killing by nitric oxide produced from IFN-gamma-activated macrophages.
Fan
Z H
ZH
Department of Immunology, Nagoya University School of Medicine, Japan.
Isobe
K
K
Kiuchi
K
K
Nakashima
I
I
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D008264:Macrophages / Q000378:metabolism*; D008516:Medicine, Chinese Traditional; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D009569:Nitric Oxide / Q000378:metabolism*; D009573:Nitrites / Q000378:metabolism; D005894:Panax; D008517:Phytotherapy; D010946:Plants, Medicinal
D009573:Nitrites; D009569:Nitric Oxide