title: Characterization of an ATP-modulated large conductance Ca(2+)-activated K+ channel present in rat cortical neurones.
abstract: 1. Single channel current recordings were used to study the characteristics of a large conductance Ca(2+)-activated K+ (BKCa) channel present in neurones acutely dissociated from the rat motor cortex. Application of ATP to the intracellular surface of excised inside-out patches produced a large, concentration-dependent increase in BKCa channel activity. 2. This ATP-mediated activation was dependent upon the presence of Mg2+ in the intracellular bathing solution and was diminished by the phosphatases 2,3-butanedione monoxime (BDM) or alkaline phosphatase and by the protein kinase inhibitors staurosporine, H-7 and PKI. 3. ADP stimulated BKCa channel activity in a Mg(2+)-dependent manner, an action also inhibited by the concomitant application of PKI or BDM. The effect of ADP was reduced by application of hexokinase and glucose or by application of the adenylate kinase inhibitor Ap5A. 4. Of other nucleotides tested, only CTP consistently activated BKCa channel activity. 5. Using the cell-attached configuration, bath application of forskolin or dibutyryl cAMP stimulated BKCa channel activity. 6. It is concluded that BKCa channel activity in the rat motor cortex is subject to modulation by the activity of a closely associated kinase. The ability of cAMP activators to stimulate BKCa channel activity in the intact cell suggests that this system may be of physiological importance.
authors.affiliation: Department of Pharmacology, University of Cambridge, UK.
authors.forename: K
authors.initials: K
authors.lastname: Lee
authors.forename: I C
authors.initials: IC
authors.lastname: Rowe
authors.forename: M L
authors.initials: ML
authors.lastname: Ashford
mesh_terms: D000255:Adenosine Triphosphate / Q000494:pharmacology / Q000502:physiology*; D000818:Animals; D002118:Calcium / Q000502:physiology*; D004594:Electrophysiology; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D008274:Magnesium / Q000494:pharmacology; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D008566:Membranes / Q000187:drug effects / Q000378:metabolism; D009044:Motor Cortex / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D009711:Nucleotides / Q000494:pharmacology; D010744:Phosphoric Monoester Hydrolases / Q000037:antagonists & inhibitors / Q000494:pharmacology; D010766:Phosphorylation; D015221:Potassium Channels / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D047428:Protein Kinase Inhibitors; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D009711:Nucleotides; D015221:Potassium Channels; D047428:Protein Kinase Inhibitors; D000255:Adenosine Triphosphate; D010744:Phosphoric Monoester Hydrolases; D008274:Magnesium; D002118:Calcium