Effects of iloprost on salt-sensitive Dahl rats.
The main pharmacological effects of iloprost, a stable prostacyclin (PGI2) derivative, are inhibition of platelet aggregation and vasodilatation, both effects being mediated by an activation of adenylate cyclase/cAMP complex. Na+ has an opposite, inhibiting adenylate cyclase effect on plasma membrane. The effect of iloprost (1 microgram/kg/min) was investigated on mean arterial pressure, bleeding time, in vivo platelet aggregation to collagen and plasma thromboxane A2/PGI2 in genetically salt-sensitive Dahl rats (12) and their salt-resistant controls (12). In this subhypotensive dose, iloprost significantly increased bleeding time and plasma PGI2 levels in both groups and significantly decreased TXA2 levels. In vivo platelet aggregation to collagen was significantly inhibited in both groups. All described effects were more distinct in salt-sensitive, compared to salt-resistant rats (differences were significant). A modulation of Na+ effect by iloprost, via adenylate cyclase mechanism, was suggested.
Somova
L
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Department of Human Physiology and Physiological Chemistry, University of Durban-Westville, South Africa.
D015121:6-Ketoprostaglandin F1 alpha / Q000097:blood; D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D001760:Bleeding Time; D001792:Blood Platelets / Q000187:drug effects; D001794:Blood Pressure / Q000187:drug effects; D011464:Epoprostenol / Q000097:blood; D016285:Iloprost / Q000008:administration & dosage / Q000494:pharmacology*; D010975:Platelet Aggregation Inhibitors / Q000008:administration & dosage / Q000494:pharmacology*; D010976:Platelet Count / Q000187:drug effects; D051381:Rats; D012044:Regression Analysis; D012965:Sodium Chloride / Q000633:toxicity; D013928:Thromboxane A2 / Q000097:blood; D013929:Thromboxane B2 / Q000097:blood
D010975:Platelet Aggregation Inhibitors; D012965:Sodium Chloride; D013929:Thromboxane B2; D013928:Thromboxane A2; D015121:6-Ketoprostaglandin F1 alpha; D011464:Epoprostenol; D000262:Adenylyl Cyclases; D016285:Iloprost