title: Characterization of a human chromosome 22 enriched bacterial artificial chromosome sublibrary.
abstract: Selection of chromosomal sublibraries from total human genomic libraries is critical for chromosome-based physical mapping approaches. We have previously reported a method of screening total human genomic library using flow sorted chromosomal DNA as a hybridization probe and selection of a human chromosome 22-enriched sublibrary from a total human bacterial artificial chromosome (BAC) library (Nucleic Acids Res 1995; 23: 1838-39). We describe here further details of the method of construction as well as characterization of the chromosome 22-enriched sublibrary thus constructed. Nearly 40% of the BAC clones that have been mapped by fluorescence in situ hybridization (FISH) analysis were localized to chromosome 22. By screening the sublibrary using chromosome 22-specific hybridization probes, we estimated that the sublibrary represents at least 2.5 x coverage of chromosome 22. This is in good agreement with the results from FISH mapping experiments. FISH map data also indicate that chromosome 22-specific BACs in the sublibrary represent all the subregions of chromosome 22.
authors.affiliation: Division of Biology and Beckmann Institute, California Institute of Technology, Pasadena 91125, USA.
authors.forename: U J
authors.initials: UJ
authors.lastname: Kim
authors.forename: H
authors.initials: H
authors.lastname: Shizuya
authors.forename: X N
authors.initials: XN
authors.lastname: Chen
authors.forename: L
authors.initials: L
authors.lastname: Deaven
authors.forename: S
authors.initials: S
authors.lastname: Speicher
authors.forename: J
authors.initials: J
authors.lastname: Solomon
authors.forename: J
authors.initials: J
authors.lastname: Korenberg
authors.forename: M I
authors.initials: MI
authors.lastname: Simon
mesh_terms: D000818:Animals; D002876:Chromosomes, Bacterial; D002892:Chromosomes, Human, Pair 22; D005434:Flow Cytometry; D015723:Gene Library; D015894:Genome, Human; D006801:Humans; D007091:Image Processing, Computer-Assisted; D017404:In Situ Hybridization, Fluorescence