Characterization of a human chromosome 22 enriched bacterial artificial chromosome sublibrary.
Selection of chromosomal sublibraries from total human genomic libraries is critical for chromosome-based physical mapping approaches. We have previously reported a method of screening total human genomic library using flow sorted chromosomal DNA as a hybridization probe and selection of a human chromosome 22-enriched sublibrary from a total human bacterial artificial chromosome (BAC) library (Nucleic Acids Res 1995; 23: 1838-39). We describe here further details of the method of construction as well as characterization of the chromosome 22-enriched sublibrary thus constructed. Nearly 40% of the BAC clones that have been mapped by fluorescence in situ hybridization (FISH) analysis were localized to chromosome 22. By screening the sublibrary using chromosome 22-specific hybridization probes, we estimated that the sublibrary represents at least 2.5 x coverage of chromosome 22. This is in good agreement with the results from FISH mapping experiments. FISH map data also indicate that chromosome 22-specific BACs in the sublibrary represent all the subregions of chromosome 22.
Kim
U J
UJ
Division of Biology and Beckmann Institute, California Institute of Technology, Pasadena 91125, USA.
Shizuya
H
H
Chen
X N
XN
Deaven
L
L
Speicher
S
S
Solomon
J
J
Korenberg
J
J
Simon
M I
MI
D000818:Animals; D002876:Chromosomes, Bacterial; D002892:Chromosomes, Human, Pair 22; D005434:Flow Cytometry; D015723:Gene Library; D015894:Genome, Human; D006801:Humans; D007091:Image Processing, Computer-Assisted; D017404:In Situ Hybridization, Fluorescence