Efficient one-step direct labelling of recombinant antibodies with technetium-99m.
High-affinity bacterially expressed antibody fragments can nowadays be cloned from established hybridomas or, more conveniently, isolated directly from antibody libraries displayed on filamentous phage. Such antibodies can be tagged with C-terminal peptide tags containing one cysteine residue, which represents a convenient functionalisation site for a number of applications, including technetium-99m labelling. Here we describe a simple one-step method for 99mTc labelling of cysteine-tagged recombinant antibodies with more than 50% radionuclide incorporation. The labelled antibodies displayed full retention of immuoreactivity and good stability.
Liberatore
M
M
Dipartimento di Medicina Sperimentale, Sezione di Medicina Nucleare, Policlinico Umberto I, Universita' di Roma "La Sapienza", Via Regina Elena 324, I-00161, Roma, Italy.
Neri
D
D
Neri
G
G
Pini
A
A
Iurilli
A P
AP
Ponzo
F
F
Spampinato
G
G
Padula
F
F
Pala
A
A
Colella
A C
AC
D000907:Antibodies, Bacterial; D000915:Antibody Affinity; D018796:Immunoconjugates; D007128:Immunoglobulin Fragments; D007553:Isotope Labeling / Q000379:methods*; D011994:Recombinant Proteins; D013667:Technetium
D000907:Antibodies, Bacterial; D018796:Immunoconjugates; D007128:Immunoglobulin Fragments; D011994:Recombinant Proteins; D013667:Technetium