Effect of different solubilizing agents on the aggregation state and catalytic activity of two purified rabbit cytochrome P450 isozymes, CYP1A2 (LM4) and CYP2B4 (LM2).
The effects of different ionic and nonionic detergents and lecithin/Na cholate mixtures on the aggregation state and catalytic activity of two major inducible rabbit cytochromes P450, CYP1A2 (LM4) and CYP2B4 (LM2), both of which are highly aggregated in solution, have been compared. Nonionic detergents Emulgen 913 and Lubrol PX as well as the mixture of lecithin-cholate demonstrate the ability to induce the dissociation of P450 isozymes only to dimers, accompanied by an increase in their catalytic activity, especially in lecithin proteoliposomes.
Viner
R I
RI
Laboratory of Physico-Chemistry of Biomembranes, School of Biology, Moscow State University, Russia.
Novikov
K N
KN
Ritov
V B
VB
Kagan
V E
VE
Alterman
M A
MA
D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases; D019388:Cytochrome P-450 CYP1A2; D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry / Q000378:metabolism*; D003902:Detergents / Q000737:chemistry; D007700:Kinetics; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology; D010088:Oxidoreductases / Q000737:chemistry / Q000378:metabolism*; D010713:Phosphatidylcholines / Q000737:chemistry; D011485:Protein Binding; D011817:Rabbits; D012995:Solubility; D013250:Steroid Hydroxylases / Q000737:chemistry / Q000378:metabolism*
D003902:Detergents; D010713:Phosphatidylcholines; D003577:Cytochrome P-450 Enzyme System; D010088:Oxidoreductases; D013250:Steroid Hydroxylases; D001189:Aryl Hydrocarbon Hydroxylases; D019388:Cytochrome P-450 CYP1A2; C050356:steroid 15-alpha-hydroxylase