title: Intraluminal flow preferentially increases net sodium uptake in the rabbit facial vein.
abstract: The infusion of physiological salt solution into the lumen of ring segments of the isolated rabbit facial vein induces an increase in vessel wall tone. Changes in 22Na+ and 45Ca2+ net uptake as well as 22Na+ unidirectional efflux in response to flow in this vessel were compared to the response to histamine (1 microM) and to angiotensin II (0.1 microM). 22Na+ net uptake per unit force developed in response to flow was 7.9-fold greater than that for histamine (1 microM) and twice that for angiotensin II (0.1 microM). In comparison, 45Ca2+ net uptake per unit force in response to flow, histamine and K+ were similar. Flow-induced contraction and the related 45Ca2+ and 22Na+ net uptake were decreased by amiloride (30 microM) and methyl isobutyl amiloride (30 microM) without changes in 45Ca2+ or 22Na+ net uptake per unit force. Ouabain (10 microM) enhanced flow-induced contraction and the related 22Na+ net uptake. Both ouabain (10 microM) and Bay K 8644 (1 microM) increased flow-induced contraction and the related 45Ca2+ net uptake whereas they decreased the 45Ca2+ net uptake per unit force. Flow, histamine and angiotensin II increased the unidirectional efflux of 22Na+. This increase in 22Na+ unidirectional efflux was attenuated by ouabain (10 microM). These experiments demonstrate that the increases in wall force due to flow, histamine and angiotensin II are associated with increases in 22Na+ net uptake and unidirectional efflux. Flow preferentially promotes the entry of Na+, compared to histamine and angiotensin II.
authors.affiliation: Institut National de la Santé et de la Recherche Médicale Unit 141, Hôpital Lariboisière, Paris, France.
authors.forename: D
authors.initials: D
authors.lastname: Henrion
authors.forename: J A
authors.initials: JA
authors.lastname: Bevan
mesh_terms: D000804:Angiotensin II / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000493:pharmacokinetics; D002132:Calcium Radioisotopes / Q000493:pharmacokinetics; D002610:Cheek / Q000098:blood supply; D005145:Face / Q000098:blood supply*; D006632:Histamine / Q000494:pharmacology; D009119:Muscle Contraction / Q000187:drug effects; D009131:Muscle, Smooth, Vascular / Q000166:cytology; D011817:Rabbits; D012039:Regional Blood Flow / Q000502:physiology; D012964:Sodium / Q000493:pharmacokinetics*; D012979:Sodium Radioisotopes / Q000493:pharmacokinetics; D014680:Veins / Q000187:drug effects / Q000502:physiology
chemical_list: D002132:Calcium Radioisotopes; D012979:Sodium Radioisotopes; D000804:Angiotensin II; D006632:Histamine; D012964:Sodium; D002118:Calcium