Antigen specificity of the ovine cytotoxic T lymphocyte response to bluetongue virus.
Bluetongue virus (BTV), an arbovirus transmitted by midges, can cause serious disease in sheep. Both virus neutralizing antibody and cytotoxic T lymphocytes (CTL) have been shown to have a role in protective immunity. In this study, the antigen specificity of CTL from BTV-immune sheep has been determined using recombinant vaccinia viruses expressing individual BTV antigens. The results show that, in the sheep studied thus far, the serotype-specific outer coat protein, VP2, and the non-structural protein, NS1 are major immunogens for CTL, with VP5 (an outer coat protein) and NS3 being minor immunogens. No VP7 (a major group-reactive inner coat protein) specific CTL were detected. The CTL from sheep immunized with serotype 1 were cross-reactive and able to recognize target cells infected with other BTV serotypes. Further work demonstrated that the cross-reactive CTL recognized NS1, but not VP2.
Andrew
M
M
CSIRO Australian Animal Health Laboratory, Geelong, Vic., Australia.
Whiteley
P
P
Janardhana
V
V
Lobato
Z
Z
Gould
A
A
Coupar
B
B
D000818:Animals; D000956:Antigens, Viral / Q000096:biosynthesis / Q000276:immunology*; D001819:Bluetongue / Q000276:immunology*; D001820:Bluetongue virus / Q000276:immunology*; D016827:CD8 Antigens / Q000276:immunology; D002213:Capsid / Q000276:immunology; D002478:Cells, Cultured; D003429:Cross Reactions / Q000276:immunology; D000939:Epitopes; D005260:Female; D005347:Fibroblasts; D007114:Immunization / Q000662:veterinary; D007700:Kinetics; D008212:Lymphocyte Depletion; D008285:Major Histocompatibility Complex / Q000276:immunology; D012756:Sheep; D012867:Skin / Q000166:cytology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014616:Vaccinia virus / Q000378:metabolism; D017361:Viral Nonstructural Proteins / Q000276:immunology; D014766:Viremia / Q000276:immunology
D000956:Antigens, Viral; D016827:CD8 Antigens; D000939:Epitopes; D017361:Viral Nonstructural Proteins