Modifications of myelin basic protein in DM20 transgenic mice are similar to those in myelin basic protein from multiple sclerosis.
Transgenic mice containing different numbers of transgenes (2-70) of the myelin proteolipid protein DM20 were phenotypically normal up to 3 mo of age, after which the mice containing 70 copies of the transgene spontaneously demyelinated and died at 10-12 mo. Since we demonstrated that demyelination in multiple sclerosis involved specific chemical changes in myelin basic protein (MBP), we investigated the MBP in our transgenic line for similar changes. Both the total amount of MBP in brain and the MBP mRNA levels were unaffected at the different ages. All the isoforms (14-21 kD) of MBP were present, but the microheterogeneity (a posttranslational event) was changed resulting in a higher proportion of the less cationic components reminiscent of the changes in MBP found in multiple sclerosis. An increased amount of the citrullinated form of MBP was found by Western blot analysis. Immunogold labeling of cryosections of brain revealed a greater density of particles with the anticitrulline antibody at 10 mo and that the levels of peptidylarginine deiminase (which deiminates protein-bound arginine to citrulline) were increased. This stable transgenic line represents a useful animal model for the human disease multiple sclerosis.
Mastronardi
F G
FG
Department of Biochemistry, Hospital for Sick Children, Toronto, Canada.
Mak
B
B
Ackerley
C A
CA
Roots
B I
BI
Moscarello
M A
MA
D000818:Animals; D002956:Citrulline / Q000737:chemistry; D003711:Demyelinating Diseases / Q000378:metabolism*; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000503:physiopathology; D018628:Gene Dosage; D006867:Hydrolases / Q000378:metabolism; D007526:Isoelectric Point; D051379:Mice; D008817:Mice, Mutant Strains; D008822:Mice, Transgenic; D009103:Multiple Sclerosis / Q000378:metabolism*; D004676:Myelin Basic Protein / Q000737:chemistry*; D018991:Myelin Proteolipid Protein / Q000235:genetics; D000080002:Protein-Arginine Deiminase Type 4; D000076342:Protein-Arginine Deiminases
D004676:Myelin Basic Protein; D018991:Myelin Proteolipid Protein; D002956:Citrulline; D006867:Hydrolases; C000629235:PADI4 protein, human; D000080002:Protein-Arginine Deiminase Type 4; D000076342:Protein-Arginine Deiminases; C552151:peptidylarginine deiminase 4, mouse