Establishment of immortal hepatocytes from a CYP3A7-transgenic/p53-knockout mouse.
Immortal hepatocytes were established from a CYP3A7-transgenic/p53-knockout mouse. The cells could be maintained with shorter doubling time after culture for 1 month. Detectable amounts of CYP3A7 mRNA and the activity of 7-propoxycoumarin O-depropylase, one of the representative CYP3A activities, were present in the immortal hepatocytes. Albumin mRNA, which is specifically expressed in the liver, remained in these cells.
Shimoji
M
M
Division of Drug Metabolism, Faculty of Pharmaceutical Sciences, Hokkaido University, Japan.
Hattori
K
K
Itoh
S
S
Nakayama
K
K
Katsuki
M
M
Aizawa
S
S
Yokoi
T
T
Kamataki
T
T
D015660:7-Alkoxycoumarin O-Dealkylase / Q000235:genetics*; D000818:Animals; D002478:Cells, Cultured; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D016158:Genes, p53; D006801:Humans; D008099:Liver / Q000166:cytology* / Q000201:enzymology; D051379:Mice; D018345:Mice, Knockout; D008822:Mice, Transgenic; D012333:RNA, Messenger / Q000235:genetics
D012333:RNA, Messenger; D003577:Cytochrome P-450 Enzyme System; D015660:7-Alkoxycoumarin O-Dealkylase