Spectrofluorimetric determination of paracetamol in pharmaceuticals and biological fluids.
A spectrofluorimetric method for the determination of paracetamol is presented, based on the oxidation of the analyte to give the fluorophore 2,2'-dihydroxy-5,5'-diacetyldiaminebiphenyl. Sodium hypochlorite was used as an oxidizing reagent and the optimum pH was found to be 10.0 (sodium carbonate-boric acid buffer solution). The linear concentration range of application was 0.1-100.0 micrograms ml-1 of paracetamol, the detection limit 0.01 micrograms ml-1 and the relative standard deviation 1.2%. The method has been satisfactorily applied to the determination of paracetamol in pharmaceutical formulations and biological fluids.
Vilchez
J L
JL
Departamento de Química Analítica, Universidad de Granada, Spain.
Blanc
R
R
Avidad
R
R
Navalón
A
A
D000082:Acetaminophen / Q000008:administration & dosage / Q000032:analysis* / Q000097:blood / Q000652:urine; D000284:Administration, Oral; D018712:Analgesics, Non-Narcotic / Q000008:administration & dosage / Q000032:analysis* / Q000097:blood / Q000652:urine; D001713:Biphenyl Compounds / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010084:Oxidation-Reduction; D012973:Sodium Hypochlorite / Q000737:chemistry; D013050:Spectrometry, Fluorescence / Q000379:methods*
D018712:Analgesics, Non-Narcotic; D001713:Biphenyl Compounds; D000082:Acetaminophen; C098035:2,2'-dihydroxy-5,5'-diacetyldiaminebiphenyl; D012973:Sodium Hypochlorite