Effect of E2 envelope glycoprotein cytoplasmic domain mutations on Sindbis virus pathogenesis.
The cytoplasmic domain of the E2 envelope glycoprotein is important in Sindbis virus assembly, but little is known about its role in the pathogenesis of Sindbis virus encephalitis. To investigate its role in viral pathogenesis, we constructed six recombinant viruses containing site mutations in the E2 cytoplasmic domain, using the neurovirulent background strain, TE12. Our findings demonstrate that the E2 cytoplasmic domain is a determinant of Sindbis virus growth and neurovirulence in suckling mice as well as persistent infection in weanling scid mice. They also suggest that the tyrosine, serine, or threonine residues are not essential for replication in mouse brain or anti-E2 monoclonal antibody-mediated restriction of Sindbis virus replication.
Levine
B
B
Department of Medicine, Columbia University College of Physicians & Surgeons, New York, New York 10032, USA.
Jiang
H H
HH
Kleeman
L
L
Yang
G
G
D018354:Alphavirus Infections / Q000821:virology*; D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D001665:Binding Sites; D001921:Brain / Q000821:virology; D002460:Cell Line; D003593:Cytoplasm; D018792:Encephalitis, Viral / Q000821:virology*; D051379:Mice; D016513:Mice, SCID; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D012845:Sindbis Virus / Q000235:genetics / Q000472:pathogenicity*; D014759:Viral Envelope Proteins / Q000235:genetics / Q000378:metabolism*; D014774:Virulence / Q000235:genetics; D017735:Virus Latency / Q000235:genetics; D014779:Virus Replication
D000914:Antibodies, Viral; D014759:Viral Envelope Proteins; C064950:glycoprotein E2, Sindbis virus