Identification of influenza C virus phosphoproteins.
The HMV-II cells infected with influenza C virus were labeled with inorganic [32P]phosphate to identify phosphorylated proteins. Analysis by radioimmunoprecipitation with antiviral serum or monoclonal antibodies revealed that three major structural proteins of the virus, hemagglutinin-esterase (HE), nucleoprotein (NP), and matrix protein (M1) are all phosphorylated in both infected cells and virions. It was also observed that, in the presence of trypsin (10 micrograms/ml), the unphosphorylated form of the HE glycoprotein was cleaved efficiently whereas the phosphorylated form was not, raising the possibility that phosphorylation of HE may influence its susceptibility to degradation by proteolytic enzymes.
Nishimura
H
H
Department of Bacteriology, Yamagata University School of Medicine, Japan.
Sugawara
K
K
Gao
P
P
Muraki
Y
Y
Hongo
S
S
Kitame
F
F
Nakamura
K
K
D000911:Antibodies, Monoclonal; D004591:Electrophoresis, Polyacrylamide Gel; D006389:Hemagglutinins, Viral / Q000032:analysis / Q000378:metabolism; D006801:Humans; D009982:Gammainfluenzavirus / Q000737:chemistry* / Q000502:physiology; D009698:Nucleoproteins / Q000032:analysis / Q000378:metabolism; D010710:Phosphates / Q000378:metabolism; D010750:Phosphoproteins / Q000032:analysis*; D010766:Phosphorylation; D015531:Radioimmunoprecipitation Assay; D014407:Tumor Cells, Cultured / Q000821:virology; D014760:Viral Fusion Proteins; D014763:Viral Matrix Proteins / Q000032:analysis / Q000378:metabolism; D014764:Viral Proteins / Q000032:analysis / Q000378:metabolism
D000911:Antibodies, Monoclonal; D006389:Hemagglutinins, Viral; D009698:Nucleoproteins; D010710:Phosphates; D010750:Phosphoproteins; D014760:Viral Fusion Proteins; D014763:Viral Matrix Proteins; D014764:Viral Proteins; C060814:hemagglutinin esterase