title: Mitochondrial alleles of Simulium damnosum sensu lato infected with Onchocerca volvulus.
abstract: Onchocerca volvulus infected Simulium damnosum s.l. were analysed by directed heteroduplex analysis. Of 73 infected flies, 68 produced heteroduplex products identical to those previously identified. All 6 major sibling species, except S. leonense, were present in this group. In the 5 remaining flies, 2 new heteroduplex patterns were noted. Molecular phylogenetic analysis of these samples suggested that they belonged to the S. squamosum/S. yahense subcomplex. The ability to reliably genotype adult flies will permit studies of the vectorial capacity of the sibling species of S. damnosum s.l. for the blinding and non-blinding strains of O. volvulus.
authors.affiliation: Division of Geographic Medicine, University of Alabama at Birmingham 35294, USA.
authors.forename: J
authors.initials: J
authors.lastname: Tang
authors.forename: L
authors.initials: L
authors.lastname: Toé
authors.forename: C
authors.initials: C
authors.lastname: Back
authors.forename: T R
authors.initials: TR
authors.lastname: Unnasch
mesh_terms: D000483:Alleles; D000818:Animals; D001483:Base Sequence; D004272:DNA, Mitochondrial; D008928:Mitochondria / Q000235:genetics*; D008969:Molecular Sequence Data; D017181:Onchocerca volvulus / Q000302:isolation & purification; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012843:Simuliidae / Q000235:genetics* / Q000469:parasitology
chemical_list: D004272:DNA, Mitochondrial