title: An in vitro investigation of the role of high molecular weight human salivary sulphated glycoprotein in periodontal wound healing.
abstract: High molecular weight human salivary sulphated glycoprotein (SGP) inhibits attachment of fibroblasts to cementum in vitro and thus may enhance periodontal wound healing by repair with long junctional epithelium. However, competitive inhibition by serum constituents might prevent adequate binding of SGP for this effect to occur in vivo. The primary aim of this study was to investigate the co-adsorption in vitro, of SGP, fibronectin (FN), and albumin (ALB) to synthetic hydroxyapatite (HA) from solutions of SGP/FN (62.5/2 micrograms/mL respectively), or SGP/FN/ALB (62.5/2/4 micrograms/mL), or from individual solutions of the agents as controls: Desorption of SGP and FN was studied by preabsorbing HA with SGP or FN and subsequently exposing it to the other agent, or to buffer only as control. Adsorbates were assayed after incubation periods of up to 26 hours using specific ELISAs. SGP displaced previously adsorbed FN after 3 hours and significantly inhibited adsorption of FN and ALB compared with controls. Neither FN or ALB had a significant effect on SGP adsorption. These results are consistent with the possibility that salivary adsorption to cementum during surgery might interfere with repopulation of the root by connective tissue cells and thus contribute to wound healing by repair rather than regeneration. Separate studies taken to validate the ELISA used for SGP determination showed that HA-bound SGP contained all constituents of native SGP as revealed by SDS-PAGE and that ion exchange chromatography of SGP gave 6 fractions (I through VI), of which only the most ionic (VI) was able to inhibit cell attachment in vitro.
authors.affiliation: Department of Clinical Dental Sciences, School of Dentistry, University of Liverpool, England.
authors.forename: A
authors.initials: A
authors.lastname: Zentner
authors.forename: T G
authors.initials: TG
authors.lastname: Heaney
mesh_terms: D000017:ABO Blood-Group System / Q000502:physiology; D000327:Adsorption; D001667:Binding, Competitive; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology*; D002478:Cells, Cultured; D002852:Chromatography, Ion Exchange; D003739:Dental Cementum / Q000378:metabolism; D017886:Durapatite; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005347:Fibroblasts / Q000187:drug effects / Q000502:physiology; D005353:Fibronectins / Q000378:metabolism; D005881:Gingiva / Q000166:cytology; D006023:Glycoproteins / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D006801:Humans; D007700:Kinetics; D008970:Molecular Weight; D010519:Periodontium / Q000187:drug effects / Q000502:physiology*; D011485:Protein Binding; D012471:Salivary Proteins and Peptides / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D012709:Serum Albumin / Q000378:metabolism; D018709:Statistics, Nonparametric; D013455:Sulfur; D014945:Wound Healing / Q000187:drug effects / Q000502:physiology*
chemical_list: D000017:ABO Blood-Group System; D005353:Fibronectins; D006023:Glycoproteins; D012471:Salivary Proteins and Peptides; D012709:Serum Albumin; D013455:Sulfur; D017886:Durapatite