title: A mini Cl- channel sensitive to external pH in the basolateral membrane of guinea-pig parietal cells.
abstract: 1. Voltage-independent whole-cell Cl- currents were recorded from both single, isolated parietal cells and parietal cells within gastric glands obtained from the fundus of guinea-pig stomach. 2. The Cl- currents were rapidly suppressed by a Cl- channel blocker, NPPB (5-nitro-2-(3-phenylpropylamino)-benzoate), added to the (basolateral) bathing solution in a concentration-dependent manner with a half-maximal inhibition concentration of 12 microM. 3. The selectivity sequence among anions was I- > Br- > Cl- > F-, corresponding to Eisenman's sequence I. 4. The Cl- currents were independent of cytosolic Ca2+, cyclic AMP, cyclic GMP, GTP-gamma-S and cell volume, and were not affected by application of acid secretagogues, omeprazol, arachidonic acid or prostaglandin E2. 5. Reduction of pH in the (basolateral) bathing solution immediately inhibited the Cl- current with a pK (-log of KD) of 6.3, whereas changes in intracellular pH had no effect. 6. The single-channel conductance was estimated to be 0.46-0.6 pS by variance noise analysis during inhibition of whole-cell Cl- currents by NPPB or acidic pH. 7. It is concluded that pH-sensitive 'mini' Cl- channels, with a sub-picosiemens unitary conductance, exist in the basolateral membrane of guinea-pig parietal cells.
authors.affiliation: Department of Internal Medicine, Faculty of Medicine, Kyoto University, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Kajita
authors.forename: S
authors.initials: S
authors.lastname: Morishima
authors.forename: Y
authors.initials: Y
authors.lastname: Shirakata
authors.forename: T
authors.initials: T
authors.lastname: Kotera
authors.forename: S
authors.initials: S
authors.lastname: Ueda
authors.forename: M
authors.initials: M
authors.lastname: Okuma
authors.forename: Y
authors.initials: Y
authors.lastname: Okada
mesh_terms: D000818:Animals; D000838:Anions / Q000378:metabolism; D002462:Cell Membrane / Q000502:physiology / Q000648:ultrastructure; D018118:Chloride Channels / Q000037:antagonists & inhibitors / Q000502:physiology*; D004594:Electrophysiology; D006168:Guinea Pigs; D006863:Hydrogen-Ion Concentration; D008297:Male; D009579:Nitrobenzoates / Q000494:pharmacology; D010295:Parietal Cells, Gastric / Q000166:cytology / Q000502:physiology / Q000648:ultrastructure*; D012680:Sensitivity and Specificity
chemical_list: D000838:Anions; D018118:Chloride Channels; D009579:Nitrobenzoates; C058176:5-nitro-2-(3-phenylpropylamino)benzoic acid