[Biological monitoring of exposure to alkylating xenobiotics by determining them in complexes with plasma proteins, hemoglobin, mercapturic acids from urine of rats and industrial workers. I. Acrylonitrile].
A parent molecule of acrylonitrile (cyanoethylene, CE) may be isolated from its the model compound S-(2-cyanoethylene)-1-cysteine, as well as from its N-acetyl derivative and hemoglobin (Hb) and plasma proteins from the intoxicated animals and workers engaged in manufacturing synthetic rubber made from acrylonitrile. The developed procedure consists in the 1.5% H2O2 oxidation of a CE adduct for 30 min, followed by addition of a sample into the gas chromatographic injector at 250 degrees C, resulting in the release of CE from the adducts and in the current ingress of carrier gas into the capillary column in order to separate and determine with a FTD detector. There was a dose-response relationship between the formation of CE adducts with rat blood proteins, urinary mercapturic acids when the poison was given in a dose of 1-50 mg/kg. CE covalently bound to Hb was detected in the workers exposed to its monomer in working places.
Ivanov
V V
VV
Kanazawa University, Japan.
Hashimoto
K
K
Inomata
K
K
Kawai
T
T
Mizunuma
K
K
Klimatskaia
L G
LG
D000111:Acetylcysteine / Q000097:blood / Q000737:chemistry* / Q000652:urine; D000181:Acrylonitrile / Q000737:chemistry* / Q000633:toxicity; D000477:Alkylating Agents / Q000737:chemistry* / Q000633:toxicity; D000818:Animals; D001798:Blood Proteins / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D006454:Hemoglobins / Q000737:chemistry*; D006801:Humans; D008297:Male; D016273:Occupational Exposure; D051381:Rats; D017208:Rats, Wistar; D015262:Xenobiotics / Q000737:chemistry / Q000633:toxicity
D000477:Alkylating Agents; D001798:Blood Proteins; D006454:Hemoglobins; D015262:Xenobiotics; D000181:Acrylonitrile; D000111:Acetylcysteine
Biologicheskiĭ monitoring vozdeĭstviia alkiliruiushchikh ksenobiotikov putem ikh opredeleniia v soedineniiakh s belkami plazmy, gemoglobinom, merkapturovykh kislotakh mochi krys i promyshlennykh rabochikh. I. Akrilonitril.