Comparative changes in the liver of female Fischer-344 rats after short-term feeding of a semipurified or a semisynthetic L-amino acid-defined choline-deficient diet.
Groups of female Fischer-344 rats were fed a semipurified choline-deficient (CD) diet, or a semisynthetic L-amino acid-defined choline-deficient (CDAA) diet, for up to 12 wk and effects of the 2 diets on the liver were compared. Steatosis was diffuse and more severe throughout in rats fed the CDAA diet than in rats fed the CD diet. Greater elevations in serum aspartate and alanine aminotransferase activities were also present in the former rats, along with higher 2-bromodeoxyuridine labeling indices in the liver. Discrete amounts of 8-hydroxyguanine were detected in liver DNA, but were not significantly different in rats fed the 2 diets, or from those present in a group of control rats killed at 0 time. Glutathione S- transferase placental form-positive focal lesions were not observed in any of the rats. The results show that the CDAA diet causes more severe degrees of steatosis and liver cell death and proliferation than the CD diet, raising the possibility that it may, in contrast to the CD diet, result in the eventual induction of hepatocellular carcinomas in female Fischer-344 rats.
Nakae
D
D
Department of Oncological Pathology, Nara Medical University, Japan.
Mizumoto
Y
Y
Andoh
N
N
Tamura
K
K
Horiguchi
K
K
Endoh
T
T
Kobayashi
E
E
Tsujiuchi
T
T
Denda
A
A
Lombardi
B
B
D000410:Alanine Transaminase / Q000097:blood; D000596:Amino Acids / Q000378:metabolism / Q000494:pharmacology*; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D001835:Body Weight / Q000187:drug effects; D016923:Cell Death / Q000187:drug effects; D002796:Choline Deficiency / Q000378:metabolism / Q000473:pathology*; D004247:DNA / Q000378:metabolism; D005260:Female; D005526:Food, Formulated; D005982:Glutathione Transferase / Q000378:metabolism; D008099:Liver / Q000378:metabolism / Q000473:pathology*; D009336:Necrosis / Q000473:pathology; D009929:Organ Size / Q000187:drug effects; D051381:Rats; D011916:Rats, Inbred F344; D014280:Triglycerides / Q000097:blood
D000596:Amino Acids; D014280:Triglycerides; D004247:DNA; D005982:Glutathione Transferase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase