Brain-derived peptides regulate the steady state levels and increase stability of the blood-brain barrier GLUT1 glucose transporter mRNA.
The blood-brain barrier (BBB) GLUT1 glucose transporter gene expression is known to be regulated by putative brain trophic factors. Therefore, the present study investigated the effect of a brain-derived peptide rich preparation containing a neurotrophic factor-like action [Cerebrolysin (Cl), EBEWE, Austria]. In cultures of brain capillary endothelial cells, Cl induced a transient increase in the abundance of BBB-GLUT1 relative to actin measured by reverse transcription-polymerase chain reaction during the first 2 h of incubation, whereas a significant reduction in the GLUT1 transcript was observed at 20 and 48 h. In addition, Cl abolished the fall in GLUT1 levels induced by actinomycin D. The present data suggest that brain-derived factors in Cl are able to modulate the expression of the BBB-GLUT1 gene increasing the BBB-GLUT1 transcript stability.
Boado
R J
RJ
Department of Medicine, UCLA School of Medicine 90024, USA.
D000596:Amino Acids / Q000494:pharmacology; D000818:Animals; D001812:Blood-Brain Barrier; D001921:Brain / Q000378:metabolism*; D002417:Cattle; D002478:Cells, Cultured; D002560:Cerebrovascular Circulation; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism; D051272:Glucose Transporter Type 1; D006706:Homeostasis; D009004:Monosaccharide Transport Proteins / Q000235:genetics*; D009479:Neuropeptides / Q000502:physiology*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism*; D014158:Transcription, Genetic
D000596:Amino Acids; D051272:Glucose Transporter Type 1; D009004:Monosaccharide Transport Proteins; D009479:Neuropeptides; D012333:RNA, Messenger; C006952:cerebrolysin