title: Influenza virus M2 protein and haemagglutinin conformation changes during intracellular transport.
abstract: The influenza virus M2 protein has an ion channel activity that permits ions to enter the virion during its uncoating and also modulates pH of intracellular compartments. M2 protein is a homotetramer consisting of either a pair of disulfide-linked dimers or a disulfide-linked tetramer. The M2 trans-membrane domain peptide adopts an alfa helical secondary structure. In polarized cells, M2 protein is expressed at the apical cell surface. The amantadine-induced, M2-mediated conversion of influenza A virus haemagglutinin (HA) to the low pH conformation occurs in an acidic trans-Golgi compartment. The M2 protein ion channel activity can affect the conformation of cleaved HA during intracellular transport. The equine influenza virus 1 HA expressed from cDNA does not require coexpression of a functional M2 protein to maintain HA in its native conformation.
authors.affiliation: Institute of Virology, Slovak Academy of Sciences, Bratislava, Slovak Republic.
authors.forename: F
authors.initials: F
authors.lastname: Ciampor
authors.forename: D
authors.initials: D
authors.lastname: Cmarko
authors.forename: J
authors.initials: J
authors.lastname: Cmarková
authors.forename: E
authors.initials: E
authors.lastname: Závodská
mesh_terms: D000818:Animals; D001692:Biological Transport; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D006389:Hemagglutinins, Viral / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D009980:Influenza A virus / Q000378:metabolism; D007473:Ion Channels; D008561:Membrane Fusion; D011487:Protein Conformation; D014763:Viral Matrix Proteins / Q000737:chemistry / Q000378:metabolism*; D000086082:Viroporin Proteins
chemical_list: D019267:Hemagglutinin Glycoproteins, Influenza Virus; D006389:Hemagglutinins, Viral; D007473:Ion Channels; D014763:Viral Matrix Proteins; C031540:M-protein, influenza virus; C488937:M2 protein, Influenza A virus; D000086082:Viroporin Proteins