[Determination of sterigmatocystin in cancerous tissues, blood and urine in patients with liver and stomach cancer].
Sterigmatocystin (ST) was determined with modified Southern-Western blot and indirect competitive enzyme-linked immunosorbent assay (IC-ELISA) for 28 specimens of cancerous tissues, 13 of blood and 20 of urine in 14 patients with liver and stomach cancer. Results showed DNA-ST adduct was detected in 14 specimens of cancerous tissues and/or pericancerous tissues. ST values were higher in four of 13 patients (65-113 micrograms/kg), as compared only in one of 14 healthy persons (68 micrograms/kg). And, ST values all were very low in urine, with a maximum of 13 micrograms/kg.
Tian
H
H
Institute of Nutrition and Food Hygiene Chinese Academy of Preventive Medicine, Beijing.
Lou
J
J
Du
C
C
D018736:DNA Adducts / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D015151:Immunoblotting / Q000379:methods; D008113:Liver Neoplasms / Q000737:chemistry*; D013241:Sterigmatocystin / Q000032:analysis*; D013274:Stomach Neoplasms / Q000737:chemistry*
D018736:DNA Adducts; D013241:Sterigmatocystin