title: Ethanol utilization regulatory protein: profile alignments give no evidence of origin through aldehyde and alcohol dehydrogenase gene fusion.
abstract: The suggestion that the ethanol regulatory protein from Aspergillus has its evolutionary origin in a gene fusion between aldehyde and alcohol dehydrogenase genes (Hawkins AR, Lamb HK, Radford A, Moore JD, 1994, Gene 146:145-158) has been tested by profile analysis with aldehyde and alcohol dehydrogenase family profiles. We show that the degree and kind of similarity observed between these profiles and the ethanol regulatory protein sequence is that expected from random sequences of the same composition. This level of similarity fails to support the suggested gene fusion.
authors.affiliation: Pittsburgh Supercomputing Center, Pennsylvania 15213, USA. nicholas@psc.edu
authors.forename: H B
authors.initials: HB
authors.lastname: Nicholas
authors.forename: B
authors.initials: B
authors.lastname: Persson
authors.forename: H
authors.initials: H
authors.lastname: Jörnvall
authors.forename: J
authors.initials: J
authors.lastname: Hempel
mesh_terms: D000426:Alcohol Dehydrogenase / Q000737:chemistry / Q000235:genetics*; D000444:Aldehyde Dehydrogenase / Q000737:chemistry / Q000235:genetics*; D000595:Amino Acid Sequence; D001233:Aspergillus nidulans / Q000737:chemistry* / Q000235:genetics; D005075:Biological Evolution; D004268:DNA-Binding Proteins / Q000235:genetics*; D005656:Fungal Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D016415:Sequence Alignment
chemical_list: C074202:ALCR protein, Aspergillus nidulans; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D000426:Alcohol Dehydrogenase; D000444:Aldehyde Dehydrogenase