Differential effects of prostaglandin E1 and prostaglandin E2 on growth and differentiation of murine myeloid leukemic cell line, M1.
Effects of prostaglandins (PGs) of the E series on growth and differentiation of murine myeloid leukemic cell line M1 were studied. PGE1, but not PGE2, inhibited the growth of M1 cells. PGE2 neither inhibited nor augmented the antiproliferative effect of PGE1. PGE1 augmented the differentiation of M1 cells into macrophage-like cells induced by interleukin 6. PGE2, however, did not exhibit any effect on the differentiation. PGE1 caused a marked increased in intracellular cAMP level in M1 cells, whereas PGE2 had no effect. These results indicate that M1 cells are able to respond only to PGE1. Radiolabeled PGE1 binding experiments, however, revealed that there was no specific binding in M1 cells, suggesting that the cells express low numbers of receptors or very low affinity receptors specific for PGE1. Stable agonists of PGI2, iloprost, cicaprost or carbacyclin, also potently inhibited the growth of M1 cells. These findings suggest that PGE1 as well as PGI2 may play a role in the differentiation of monocyte-macrophage lineage cells.
Sawamura
H
H
Department of Hygienic Chemistry, Faculty of Pharmaceutical Sciences, Nagoya City University, Aichi, Japan.
Hayashi
H
H
Onozaki
K
K
D000527:Alprostadil / Q000494:pharmacology*; D000818:Animals; D001665:Binding Sites; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D000242:Cyclic AMP / Q000096:biosynthesis; D015232:Dinoprostone / Q000819:agonists / Q000494:pharmacology*; D007424:Intracellular Fluid / Q000378:metabolism; D007951:Leukemia, Myeloid / Q000378:metabolism / Q000473:pathology*; D008264:Macrophages / Q000187:drug effects; D051379:Mice; D014407:Tumor Cells, Cultured
D000242:Cyclic AMP; D000527:Alprostadil; D015232:Dinoprostone