pH-dependent transport of procainamide in cultured renal epithelial monolayers of OK cells: consistent with nonionic diffusion.
1. Previous studies suggest that procainamide is a substrate for organic cation/proton antiport. In order to study the coupling between procainamide flux and proton flux in greater detail we investigated the effects of extracellular procainamide addition upon intracellular pH in cultured monolayers of renal OK cells. Intracellular pH was monitored by use of BCECF as a probe. 2. Apical addition of procainamide (10 mM) caused a significant alkalinisation of intracellular pH. Basolateral addition of procainamide was equally effective in raising intracellular pH. A similar alkalinisation was found in two other renal cell lines: MDCK strain 1 and LLCPK1. 3. In contrast, both tetraethylammonium and N-methylnicotinamide, archetypal substrates for organic cation/proton antiport were without effect upon intracellular pH. 4. At physiological pH values, procainamide exists as a neutral weak base (B) and its conjugate weak acid (BH+). To test which species of procainamide was responsible for the alkalinisation, experiments in which [B] was kept constant whilst [BH+] was varied from 1.15 mM to 7.25 mM were performed. The results suggested that the neutral weak base (B) was the permeant species. 5. Procainamide efflux from procainamide-loaded cell monolayers resulted in a significant acidification of intracellular pH. As with procainamide uptake, this result could be ascribed to the movement of neutral weak base. 6. These effects of procainamide upon intracellular pH are consistent with nonionic diffusion of procainamide rather than an interaction of procainamide with the organic cation/proton antiporter. In addition, the results suggest that organic cation/proton antiport is not highly expressed in OK cells.
Dudley
A J
AJ
Department of Physiological Sciences, University of Newcastle upon Tyne, Medical School.
Brown
C D
CD
D000818:Animals; D017920:Antiporters / Q000378:metabolism; D001693:Biological Transport, Active; D002412:Cations; D002455:Cell Division / Q000502:physiology; D002478:Cells, Cultured; D004058:Diffusion; D004848:Epithelium / Q000187:drug effects / Q000378:metabolism; D005452:Fluoresceins; D005456:Fluorescent Dyes; D006863:Hydrogen-Ion Concentration; D007424:Intracellular Fluid / Q000378:metabolism; D007668:Kidney / Q000187:drug effects / Q000378:metabolism*; D007700:Kinetics; D009536:Niacinamide / Q000031:analogs & derivatives / Q000494:pharmacology; D009893:Opossums; D011342:Procainamide / Q000493:pharmacokinetics* / Q000494:pharmacology; D019789:Tetraethylammonium; D013757:Tetraethylammonium Compounds / Q000493:pharmacokinetics / Q000494:pharmacology
D017920:Antiporters; D002412:Cations; D005452:Fluoresceins; D005456:Fluorescent Dyes; D013757:Tetraethylammonium Compounds; D009536:Niacinamide; D019789:Tetraethylammonium; C043829:2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein; D011342:Procainamide; C008472:N-methylnicotinamide