Phorbol ester (TPA)-induced differential modulation of cell surface antigens in human pluripotential leukemia (K-562) cell line: effects of protein kinase inhibitors with broad- and PKC selective inhibitory activity.
Phorbol ester (TPA)-induced increase in cell surface expression of adhesion structures, i.e. intercellular adhesion molecule-1 (ICAM-1, CD54), beta 2 integrin LFA-1 (CD11a), complement-regulatory cell membrane protein-protein (CD59) and leukocyte common antigen (CD45) in human erythroid/myeloid leukemia cell line K-562 was inhibited by staurosporine, an inhibitor with broad, non-selective protein kinase inhibitory profile, but not by CGP 41,251, a benzoylated staurosporine derivative with the selective protein kinase C (PKC) inhibitory activity. Neither staurosporine nor CGP 41,251 modulated TPA-induced down-regulation of transferrin receptor (CD71). These data suggest that phorbol ester-induced cell surface antigen modulations in K-562 cells are predominantly mediated by PKC-independent signalling pathways.
Hunáková
L
L
Cancer Research Institute, Slovak Academy of Sciences, Bratislava, Slovakia.
Sedlák
J
J
Klobusická
M
M
Sulíková
M
M
Chorváth
B
B
D000470:Alkaloids / Q000494:pharmacology; D015703:Antigens, CD / Q000032:analysis*; D018957:CD59 Antigens / Q000032:analysis; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis; D007938:Leukemia / Q000276:immunology*; D017493:Leukocyte Common Antigens / Q000032:analysis; D016169:Lymphocyte Function-Associated Antigen-1 / Q000032:analysis; D011493:Protein Kinase C / Q000037:antagonists & inhibitors* / Q000502:physiology*; D019311:Staurosporine / Q000031:analogs & derivatives*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D000470:Alkaloids; D015703:Antigens, CD; D018957:CD59 Antigens; D016169:Lymphocyte Function-Associated Antigen-1; D018799:Intercellular Adhesion Molecule-1; D011493:Protein Kinase C; D017493:Leukocyte Common Antigens; D019311:Staurosporine; C059539:midostaurin; D013755:Tetradecanoylphorbol Acetate