title: Cytoplasmic motility reflects phagocytic activity in alveolar macrophages from dog lungs.
abstract: To determine whether cytoplasmic motility relates to phagocytic activity of alveolar macrophages (AM), we measured the remanent field strength (RFS) from the AM containing Fe3O4 particles (5 x 10(6) cells) and the number of phagocytized fluorescent latex spheres in the AM without having Fe3O4 particles (10(6) cells) harvested by broncho-alveolar lavage from dog lungs in vitro. Cytoplasmic motility was estimated from the relaxation rate (lambda o; min-1) calculated from the decay curve of RFS and the number of phagocytized latex spheres was counted using fluorescent microscopy after the addition of latex spheres (5 x 10(7)). The tumor necrosis factor increased both lambda o and the number of phagocytized latex spheres, and cytochalasin D and colchicine decreased them in a concentration-dependent fashion. Increases and decreases in lambda o induced by drugs paralleled the number of phagocytized latex spheres. These results suggest that cytoplasmic motility reflects phagocytic activity of AM and both cytoplasmic movement and phagocytosis may be regulated by a similar mechanism in the cytoskeletal system.
authors.affiliation: Department of Geriatric Medicine, Tohoku University School of Medicine, Sendai, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Yamaya
authors.forename: T
authors.initials: T
authors.lastname: Fukushima
authors.forename: K
authors.initials: K
authors.lastname: Sekizawa
authors.forename: T
authors.initials: T
authors.lastname: Ohrui
authors.forename: H
authors.initials: H
authors.lastname: Sasaki
mesh_terms: D000818:Animals; D003078:Colchicine / Q000494:pharmacology; D015638:Cytochalasin D / Q000494:pharmacology; D003595:Cytoplasmic Streaming / Q000502:physiology; D004285:Dogs; D004305:Dose-Response Relationship, Drug; D005290:Ferric Compounds; D016676:Macrophages, Alveolar / Q000502:physiology*; D008280:Magnetics; D008856:Microscopy, Fluorescence; D008863:Microspheres; D010587:Phagocytosis / Q000502:physiology*; D011994:Recombinant Proteins / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
chemical_list: D005290:Ferric Compounds; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; C000499:ferric oxide; D015638:Cytochalasin D; D003078:Colchicine