title: [Detection and analysis of Tetrahymena pyriformis 26S ribosomal DNA domain sequences, differing in degree of evolutionary conservation].
abstract: Domains of different evolutionary conservatism were defined in the 26S rDNA sequence of T. pyriformis. The fragment of studied DNA (1212 bp) showing high evolutionary conservatism was cloned. It was shown this fragment of DNA may be used to a probe for blot-hybridization analysis of the structure of rDNA from various taxa, protists to mammals. Superconservative and hypervariable domains were defined. The first are good for the primers for PCR analysis of rDNA from various taxa, the second--for species specific primers.
authors.forename: D V
authors.initials: DV
authors.lastname: Mukha
authors.forename: A P
authors.initials: AP
authors.lastname: Sidorenko
mesh_terms: D000818:Animals; D005075:Biological Evolution; D003001:Cloning, Molecular; D004275:DNA, Ribosomal / Q000235:genetics*; D006801:Humans; D009690:Nucleic Acid Conformation; D016133:Polymerase Chain Reaction; D012335:RNA, Ribosomal / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D013769:Tetrahymena pyriformis / Q000145:classification / Q000235:genetics*
chemical_list: D004275:DNA, Ribosomal; D012335:RNA, Ribosomal; C065096:RNA, ribosomal, 26S
vernacular_title: Vyiavlenie i analyz domenov posledovatel'nosti 26S ribosomnoĭ DNK Tetrahymena pyriformis, razlichaiushchikhsia po stepeni évoliutsionnogo konservatizma.