[Effect of IL-10 on the mite antigen-induced IFN-gamma production by peripheral blood lymphocytes from individuals with bronchial asthma].
Interferon-gamma (IFN-gamma) production was dose-dependently suppressed by the addition of Dermatophagoides farinae (Df), mite antigen in peripheral blood mononuclear cells from children with bronchial asthma. By contrast, the levels of IFN-gamma did not change on stimulation with Df antigen in the normal lymphocytes from nonallergic individuals not sensitized with mite. Recombinant interleukin 10 (IL-10), when added to the normal lymphocytes, significantly reduced the production of IFN-gamma. Moreover, the decreased synthesis of IFN-gamma in patients' lymphocytes was inversely up-regulated by the treatment with anti-IL-10 antibody. Taken together, results suggest the regulatory role of IL-10 in the secretion of IFN-gamma in Df antigen-driven immune responses.
Kawano
Y
Y
Department of Pediatrics, Saitama Medical School.
Yoshizawa
I
I
Noma
T
T
D000293:Adolescent; D000328:Adult; D000818:Animals; D000941:Antigens / Q000276:immunology*; D001249:Asthma / Q000276:immunology*; D002478:Cells, Cultured; D002648:Child; D002675:Child, Preschool; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis*; D016753:Interleukin-10 / Q000494:pharmacology* / Q000502:physiology; D008214:Lymphocytes / Q000276:immunology* / Q000378:metabolism; D008297:Male; D008925:Mites / Q000276:immunology*; D011994:Recombinant Proteins
D000941:Antigens; D011994:Recombinant Proteins; D016753:Interleukin-10; D007371:Interferon-gamma