title: Interactions of human mannose-binding protein with lipoteichoic acids.
abstract: We explored the interaction of human recombinant mannose-binding protein and lipoteichoic acids (LTAs) by enzyme-linked immunosorbent assay. The best ligand was Micrococcus luteus lipomannan, followed by Enterococcus spp. LTA containing mono-, di-, and oligoglucosyl substituents. LTAs lacking terminal sugars (those of Streptococcus pyogenes and Staphylococcus aureus) or containing galactosyl substituents (those of Listeria spp. and Lactococcus spp.) were poor ligands. These results are consistent with known structural requirements for binding through the mannose-binding protein carbohydrate recognition domain.
authors.affiliation: Department of Internal Medicine, Yale University School of Medicine, New Haven, Connecticut 06510, USA.
authors.forename: V Y
authors.initials: VY
authors.lastname: Polotsky
authors.forename: W
authors.initials: W
authors.lastname: Fischer
authors.forename: R A
authors.initials: RA
authors.lastname: Ezekowitz
authors.forename: K A
authors.initials: KA
authors.lastname: Joiner
mesh_terms: D000209:Acute-Phase Proteins / Q000378:metabolism*; D001665:Binding Sites; D001667:Binding, Competitive; D002240:Carbohydrate Sequence; D002352:Carrier Proteins / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008070:Lipopolysaccharides / Q000378:metabolism*; D037241:Mannose-Binding Lectins; D008969:Molecular Sequence Data; D011485:Protein Binding; D013682:Teichoic Acids / Q000378:metabolism*
chemical_list: D000209:Acute-Phase Proteins; D002352:Carrier Proteins; D008070:Lipopolysaccharides; D037241:Mannose-Binding Lectins; D013682:Teichoic Acids; C064351:lipomannan; C009900:lipoteichoic acid