title: Detection of EBV DNA in oral squamous cell carcinomas in a black African population sample.
abstract: The purpose of this study was to determine the presence of Epstein-Barr virus (EBV) DNA in oral squamous cell carcinoma (OSCC) patients from a Black African population. Formalin fixed paraffin embedded blocks of OSCC of two randomly selected groups were investigated. Group 1 consisting of 57 blocks containing OSCC with a fragment of normal appearing adjacent/overlying epithelium. Group 2 consisted of 48 blocks containing only OSCC tissue without any normal appearing epithelium. The control group consisted of 38 non-malignant, non-viral associated lesions. A standard polymerase chain reaction (PCR) was used to amplify the Bam HI W-fragment using a nested primer set. EBV DNA was demonstrated in 14/57 (25%) blocks from Group 1, in 13/48 (27%) blocks from Group 2 and in 16/38 (42%) blocks from the control group. No evidence for a direct role of EBV in the process of malignant transformation of intraoral epithelial cells was found in this study.
authors.affiliation: Department Medical Virology, University of Stellenbosch, Tygerberg, South Africa.
authors.forename: E J
authors.initials: EJ
authors.lastname: Van Rensburg
authors.forename: S
authors.initials: S
authors.lastname: Engelbrecht
authors.forename: W
authors.initials: W
authors.lastname: Van Heerden
authors.forename: E
authors.initials: E
authors.lastname: Raubenheimer
authors.forename: B D
authors.initials: BD
authors.lastname: Schoub
mesh_terms: D000349:Africa / Q000453:epidemiology; D001483:Base Sequence; D044383:Black People / Q000235:genetics; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000821:virology*; D004279:DNA, Viral / Q000032:analysis*; D004854:Herpesvirus 4, Human / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008969:Molecular Sequence Data; D009062:Mouth Neoplasms / Q000235:genetics* / Q000821:virology*; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity
chemical_list: D004279:DNA, Viral