[Study of plant lectins from Viscum album using monoclonal antibodies].
Monoclonal antibodies (monAT) against both native (TA5, TB12) and denatured (TB33, TB35) plant toxin ML1 from Viscum album have been obtained. The interaction of monAT against native toxin with its isoforms ML2 and ML3 was investigated. It was shown that monAT TA5 to A-chain of ML1 toxin cross-reacted with ML2 and ML3 isoforms. TA5 did not inhibit enzyme activity of A-chain in cell-free rabbit reticulocyte system. It was shown that monAT TB12 reacted with galactose-binding site of B-subunit. Both monAT had no cross-reactions with plant toxin ricin. The binding constants for TA5 with ML1, ML2, ML3 respectively were 4.3.10(7) M-1, 1.2.10(7) M-1, and 0.3.10(7) M-1. The binding constants for TB12 were 2.10(7) M-1 with ML1 toxin, and more than 10(6) M-1 with ML2 and ML3. The nature of heterogeneity in ML toxin family is discussed. Test-systems for ML1 determination in different V. album extracts are suggested.
Tonevitskiĭ
A G
AG
Witten-Herdex University, Germany.
Rakhmanova
V A
VA
Shamshiev
A T
AT
Usachaeva
E A
EA
Agapov
I I
II
Prokov'ev
S A
SA
Denisenko
O N
ON
Pfuller
U
U
Eifler
R
R
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001212:Asialoglycoproteins / Q000494:pharmacology; D015153:Blotting, Western; D002474:Cell-Free System; D003429:Cross Reactions; D060748:Fetuins; D006825:Hybridomas; D037102:Lectins / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D014784:Mistletoe / Q000378:metabolism*; D037121:Plant Lectins; D010946:Plants, Medicinal; D011485:Protein Binding; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D011817:Rabbits; D012276:Ricin / Q000276:immunology; D012996:Solutions; D014118:Toxins, Biological / Q000276:immunology / Q000378:metabolism*; D000509:alpha-Fetoproteins / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D001212:Asialoglycoproteins; D060748:Fetuins; D037102:Lectins; D037121:Plant Lectins; D011500:Protein Synthesis Inhibitors; D012996:Solutions; D014118:Toxins, Biological; D000509:alpha-Fetoproteins; C017029:asialofetuin; D012276:Ricin
Issledovanie rastitel'nykh lektinov iz omely beloĭ s pomoshch'iu monoklonal'nykh antitel.