title: Importance of T-cell-dependent inflammatory reactions in the decline of microsomal cytochrome P450 concentration in the livers of rats infected with Fasciola hepatica.
abstract: The concentration of cytochrome P450, measured spectrophotometrically in microsomal preparations from the livers of rats infected with 30 metacercariae of Fasciola hepatica, declined by approximately 50% at 3 weeks post-infection. Treatment of infected rats with the anti-inflammatory agent dexamethasone (2 mg/kg at 48 h intervals for 8 days prior to assay) abolished the decline in P450 content. Assay of P450 in infected congenitally athymic (nude) rats showed normal levels. These results demonstrate that the T-cell-dependent inflammatory response in the liver of the host is a necessary factor in the development of the decline in hepatic P450, which is known to compromise the metabolism of certain drugs in infected hosts.
authors.affiliation: Division of Biochemistry and Molecular Biology, School of Life Sciences, Australian National University, Canberra, Australia.
authors.forename: F
authors.initials: F
authors.lastname: Topfer
authors.forename: L M
authors.initials: LM
authors.lastname: Lenton
authors.forename: F L
authors.initials: FL
authors.lastname: Bygrave
authors.forename: C A
authors.initials: CA
authors.lastname: Behm
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D003907:Dexamethasone / Q000494:pharmacology; D005211:Fascioliasis / Q000201:enzymology / Q000276:immunology*; D007249:Inflammation / Q000276:immunology; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology* / Q000276:immunology; D051381:Rats; D011917:Rats, Inbred Lew; D011923:Rats, Nude; D017208:Rats, Wistar; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D000893:Anti-Inflammatory Agents; D003907:Dexamethasone; D003577:Cytochrome P-450 Enzyme System