Human papillomavirus 11 E5a delays the growth restriction induced by temperature shift in temperature-sensitive simian virus 40 T antigen-immortalized keratinocytes.
The transfection with human papillomavirus type 11 E5a oncoprotein can cause longer and more active proliferation in the human keratinocytes previously transfected stably with temperature sensitive SV40 T antigen at 39 degrees C. Also, after the E5a transfection in parental temperature sensitive SV40 T antigen gene transfected cells, we observe a delay in the accumulation of p21 gene at 39 degrees C. Moreover, T antigen degradation did not occur at 39 degrees C in the E5a transfected cells as it did in the parental cells. We draw from these observations that E5a may transiently stabilize temperature sensitive T antigen and/or repress p21 synthesis.
Tsao
Y P
YP
Dept. of Microbiology and Immunology, National Defense Medical Center, Taipei, Taiwan, Republic of China.
Kuo
S W
SW
Li
S F
SF
Tsai
T C
TC
Li
L Y
LY
Chen
S L
SL
D000952:Antigens, Polyomavirus Transforming / Q000235:genetics*; D002455:Cell Division; D002460:Cell Line; D015967:Gene Expression Regulation, Viral; D005816:Genetic Complementation Test; D006801:Humans; D015603:Keratinocytes / Q000166:cytology; D009856:Oncogene Proteins, Viral / Q000235:genetics*; D009857:Oncogenes; D027383:Papillomaviridae / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D014162:Transfection
D000952:Antigens, Polyomavirus Transforming; C081917:E5A protein, Human papillomavirus type 11; D009856:Oncogene Proteins, Viral; D012333:RNA, Messenger