Early events in erythroid differentiation: accumulation of the acidic peroxidoxin (PRP/TSA/NKEF-B).
The acidic peroxidoxin [also named thiol-specific antioxidant protein (TSA) or protector protein (PRP)], which plays a role in the response against oxidative stress, is one of the major proteins of red blood cells. In this work, we show that this protein is induced at early stages of erythroid differentiation prior to haemoglobin accumulation, which suggests that it may play a role at the erythroblast stage, where haemoglobinized, nucleated and genetically active cells are submitted to a maximally dangerous oxidative stress. The early accumulation of this protein has been demonstrated both on transformed cell systems and on normal differentiating human erythroid cells. This suggests that this protein may play an important role in the differentiation of the erythroid cells.
Rabilloud
T
T
Laboratoire de Biologie Moléculaire du Cycle Cellulaire, INSERM U309, Grenoble, France.
Berthier
R
R
Vinçon
M
M
Ferbus
D
D
Goubin
G
G
Lawrence
J J
JJ
D000818:Animals; D000975:Antioxidants; D001798:Blood Proteins / Q000378:metabolism; D002454:Cell Differentiation; D002460:Cell Line; D004900:Erythroblasts / Q000166:cytology / Q000378:metabolism; D004912:Erythrocytes / Q000166:cytology / Q000378:metabolism*; D015672:Erythroid Precursor Cells / Q000166:cytology / Q000378:metabolism; D005622:Friend murine leukemia virus; D006360:Heat-Shock Proteins; D006801:Humans; D004915:Leukemia, Erythroblastic, Acute; D051379:Mice; D009363:Neoplasm Proteins; D010544:Peroxidases; D061105:Peroxiredoxin III; D054464:Peroxiredoxins; D011506:Proteins / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D000975:Antioxidants; D001798:Blood Proteins; D006360:Heat-Shock Proteins; D009363:Neoplasm Proteins; C516116:Prdx3 protein, mouse; D011506:Proteins; D010544:Peroxidases; C086065:PRDX2 protein, human; C516115:PRDX3 protein, human; D061105:Peroxiredoxin III; D054464:Peroxiredoxins