Interaction of cationic liposomes with cells of electrically active neuronal networks in culture.
Incubation of rhodamine-labeled cationic liposomes with mature murine spinal cultures results in strong fluorescence that is evenly distributed on somata and neurites of neurons in 7 different cultures. Staining of the glial carpet is minimal. Rhodamine-labeled dextran, encapsulated in liposomes, also stains neurons. Electron microscope data show external attachment and intact internalization of liposomes. Spontaneous electrical bursting activity is altered but not lost after incubation.
Azzazy
H M
HM
Department of Biological Sciences, University of North Texas, Denton 76203, USA.
Hong
K
K
Wu
M C
MC
Gross
G W
GW
D000818:Animals; D002412:Cations / Q000008:administration & dosage / Q000494:pharmacology*; D002462:Cell Membrane / Q000187:drug effects / Q000648:ultrastructure; D002478:Cells, Cultured; D004594:Electrophysiology; D004705:Endocytosis / Q000187:drug effects / Q000502:physiology; D005110:Extracellular Space / Q000187:drug effects / Q000502:physiology; D008081:Liposomes; D051379:Mice; D017348:Microscopy, Electron, Scanning Transmission; D009415:Nerve Net / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D009474:Neurons / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D012235:Rhodamines; D013116:Spinal Cord / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D013194:Staining and Labeling
D002412:Cations; D008081:Liposomes; D012235:Rhodamines