High molecular weight precursors of glucans in Saccharomyces cerevisiae.
Nascent beta-1,3 glucan synthesized by mixed membrane fractions from Saccharomyces cerevisiae was solubilized by extraction with hot SDS or urea. Nature of the material was analyzed by electrophoresis and gel filtration. As determined by gel filtration, Mr of synthesized glucans exceeded 1,500 kDa, but was below 20,000 kDa. This nascent material served as an acceptor for further glucose transfer reactions, giving rise to glucan molecules over 20,000 kDa. It is suggested that the high Mr precursor components represent protein-bound glucan molecules in transit to the cell surface.
Ruiz-Herrera
J
J
Department of Genética, Centro de Investigación y de Estudios Avanzados del IPN, Irapuato, Gto., México.
Larriba
G
G
D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005656:Fungal Proteins / Q000302:isolation & purification / Q000378:metabolism; D005936:Glucans / Q000096:biosynthesis* / Q000302:isolation & purification; D007425:Intracellular Membranes / Q000378:metabolism; D046911:Macromolecular Substances; D008565:Membrane Proteins / Q000302:isolation & purification / Q000378:metabolism; D008970:Molecular Weight; D012441:Saccharomyces cerevisiae / Q000378:metabolism* / Q000648:ultrastructure; D012995:Solubility; D047071:beta-Glucans
D005656:Fungal Proteins; D005936:Glucans; D046911:Macromolecular Substances; D008565:Membrane Proteins; D047071:beta-Glucans; C033363:beta-1,3-glucan