title: Cytochrome c oxidase subunit VIIa liver isoform. Characterization and identification of promoter elements in the bovine gene.
abstract: Cytochrome c oxidase subunit VIIa is specified by two nuclear genes, one (COX7AH) producing a heart/muscle-specific isoform and the other (COX7AL) a form expressed in all tissues. We have isolated both genes to examine their transcriptional regulation. Here, we characterize the core promoter of COX7AL and show that a 92-base pair region flanking the 5'-end promotes most of the activity of this gene. The 92-bp basal promoter contains sites for the nuclear respiratory factors NRF-1 and NRF-2, which have been shown to contribute to the transcription of a number of nuclear genes involved in mitochondrial respiratory activity, and also at least four Sp1 motifs. We show that both the NRF-1 and NRF-2 binding sites are functional in COX7AL and present evidence suggesting that interaction between the NRF-1 site and an upstream element contributes to expression.
authors.affiliation: Center for Molecular Medicine and Genetics, Wayne State University School of Medicine, USA.
authors.forename: R S
authors.initials: RS
authors.lastname: Seelan
authors.forename: L
authors.initials: L
authors.lastname: Gopalakrishnan
authors.forename: R C
authors.initials: RC
authors.lastname: Scarpulla
authors.forename: L I
authors.initials: LI
authors.lastname: Grossman
mesh_terms: D000818:Animals; D001483:Base Sequence; D001667:Binding, Competitive; D002417:Cattle; D004268:DNA-Binding Proteins / Q000378:metabolism; D003576:Electron Transport Complex IV / Q000235:genetics*; D015971:Gene Expression Regulation, Enzymologic; D007527:Isoenzymes / Q000235:genetics*; D008099:Liver / Q000201:enzymology*; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics; D015183:Restriction Mapping; D013329:Structure-Activity Relationship; D014157:Transcription Factors / Q000235:genetics
chemical_list: D004268:DNA-Binding Proteins; D007527:Isoenzymes; D012333:RNA, Messenger; D014157:Transcription Factors; D003576:Electron Transport Complex IV