Enhancement of erythroid colony growth by triiodothyronine in cell cultures from bone marrow of normal and anemic rats with chronic renal failure.
In order to make a contribution in clarifying the role of thyroid hormones on modulation of erythropoiesis and to gain a further insight on the effects of these hormones in the anemia of chronic renal failure (CRF), we studied the action of triiodo-1-thyronine (LT3) and DT3, a dextrorotary non-calorigenic isomer of T3 on late (CFU-E) and early (BFU-E) committed erythroid precursor cells from bone marrow of normal and anemic uremic rats. Cultures were prepared using the methylcellulose technique containing a standard dose (182 mU/ml) of erythropoietin (Ep), LT3 and DT3 in doses of 0.5 and 1.5 micrograms/ml. Thyroid hormones were added to cultures in the absence of Ep. Our results demonstrated that LT3 and DT3 produced a direct and significant stimulation of CFU-E formation and a moderate increase of BFU-E. A dose-correlation was apparent in cultures containing thyroid hormones. DT3 was somewhat less active than LT3. As expected, Ep also produced a significant increase in erythroid colony formation, mainly CFU-E. It is notheworthy that the effects of LT3, DT3 and Ep on erythroid colony growth were significantly higher in marrow cultures from anemic rats with CRF, indicating an increased proliferative cell kinetics of committed erythroid cells in response to these drugs.
Malgor
L A
LA
Cátedra de Farmacología, Facultad de Medicina, Universidad Nacional del Nordeste, Corrientes, Argentina.
Valsecia
M E
ME
Verges
E G
EG
de Markowsky
E E
EE
D000740:Anemia / Q000097:blood / Q000150:complications; D000818:Animals; D001854:Bone Marrow Cells; D015672:Erythroid Precursor Cells / Q000187:drug effects*; D004920:Erythropoiesis / Q000187:drug effects*; D007037:Hypothyroidism / Q000097:blood / Q000150:complications; D007676:Kidney Failure, Chronic / Q000097:blood / Q000188:drug therapy* / Q000209:etiology; D008297:Male; D051381:Rats; D014284:Triiodothyronine / Q000494:pharmacology*
D014284:Triiodothyronine