Effects of insulin on the level of glucocorticoid receptors.
The investigation of insulin effects on the glucocorticoid receptor (GR) in cytosol fraction of rat liver has been done in order to bring about more evidence on the interaction of these hormones in the regulation of metabolic processes. The experiments were performed with male Wistar rats intraperitoneally injected by 25 micrograms of insulin/100g body weight 3 or 18 hours before analysis. The obtained results show that insulin induces time dependent changes, in both, the level and functional properties of GR. The significant increase in the binding activity and receptor content was observed in both analysed time intervals of insulin treatment, while these increases were higher after 18 hours of insulin administration. These evidences suggest that mechanisms of cooperative effects of insulin and glucocorticoid on regulation of cell function involve the modulation of hormone signals transmission through increase in level and/or functionality of receptor proteins.
Isenović
E
E
Laboratory for Molecular Biology and Endocrinology, Institute for Nuclear Sciences, Vinca.
Zakula
Z
Z
Radojcić
M
M
Ribarac-Stepić
N
N
D000818:Animals; D015153:Blotting, Western; D007328:Insulin / Q000494:pharmacology*; D008099:Liver / Q000378:metabolism*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D011965:Receptors, Glucocorticoid / Q000032:analysis*
D007328:Insulin; D011965:Receptors, Glucocorticoid