Glial reactivity in the retina of adult rats.
The present study aimed to characterize the reaction of mammalian (rat) retinal macroglia (Müller cells and astrocytes) to disturbances of their environment in the form of intraorbital section of the optic nerve, intraocular insertion of a thin glass capillary (without damage to the retina) or a combination of both. Glial reactivity was assessed through the use of a battery of antibodies which recognise four different proteins--glial fibrillary protein (GFAP) and three other proteins designated respectively MA1, 4D6 and 4H11. Retinal astrocytes did not exhibit any changes in normally expressed GFAP or MA1. By contrast, the expression of GFAP and MA1 in Müller cells increased 14 days following section of the optic nerve and/or intravitreal insertions of a glass capillary. Three days postoperatively, the expression of GFAP, but not MA1, had already increased significantly in Müller cells. 4D6 and 4H11 proteins were not expressed in astrocytes. In Müller cells, the levels of these proteins increased significantly following combined optic nerve section and intraocular insertion of a glass capillary. Thus, a mechanical disturbance of the intraocular environment constitutes a more effective stimulus in increasing the expression of some Müllerian proteins than damage to the axons of retinal ganglion cells. Such changes have important implications for various ocular treatments that involve intraocular administration of drugs, as well as for the survival/regeneration potential of retinal ganglion cells undergoing Wallerian degeneration.
Huxlin
K R
KR
Department of Physiology, University of Sydney, N.S.W., Australia.
Dreher
Z
Z
Schulz
M
M
Dreher
B
B
D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage; D001369:Axons / Q000502:physiology; D015415:Biomarkers; D003429:Cross Reactions; D005123:Eye; D005260:Female; D007150:Immunohistochemistry; D007267:Injections; D008970:Molecular Weight; D009457:Neuroglia / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012160:Retina / Q000166:cytology* / Q000378:metabolism*; D014855:Wallerian Degeneration / Q000502:physiology
D000911:Antibodies, Monoclonal; D015415:Biomarkers