[Inhibition of exogenous serine proteinases by a trypsin inhibitor from the buckwheat IT-1 seeds].
The possibility of inhibition of exogenous trypsin- and chymotrypsin-like proteinases by a proteinase inhibitor from buckwheat (IT-1) seeds has been studied. The inhibition constants for bovine trypsin and alpha-chymotrypsin and human granulocyte cathepsin G by IT-1 are equal to 1.1, 67 and 200 nm, respectively. The specificity of IT-1 with regard to its primary sequence adjacent to the active center and to its homology with inhibitors pertaining to the potato inhibitor I family has been carried out. It is concluded that by virtue of the basic nature of the P1 (Arg) residue in the active center IT-1 is not capable to bind human granulocyte elastase.
Gladysheva
I P
IP
Dunaevskiĭ
Ia E
IaE
Belozerskiĭ
M A
MA
Gladyshev
D P
DP
Papisova
A I
AI
Larionova
N I
NI
D000595:Amino Acid Sequence; D000818:Animals; D056649:Cathepsin G; D002403:Cathepsins / Q000037:antagonists & inhibitors; D002417:Cattle; D002918:Chymotrypsin / Q000037:antagonists & inhibitors; D006801:Humans; D019272:Leukocyte Elastase; D008969:Molecular Sequence Data; D010196:Pancreatic Elastase / Q000037:antagonists & inhibitors / Q000378:metabolism; D012639:Seeds / Q000201:enzymology*; D017386:Sequence Homology, Amino Acid; D012697:Serine Endopeptidases; D014908:Triticum / Q000201:enzymology*; D014361:Trypsin Inhibitors / Q000737:chemistry / Q000494:pharmacology*
D014361:Trypsin Inhibitors; D002403:Cathepsins; D012697:Serine Endopeptidases; D002918:Chymotrypsin; C534094:CTSG protein, human; D056649:Cathepsin G; D010196:Pancreatic Elastase; D019272:Leukocyte Elastase
Ingibirovanie ékzogennykh serinovykh proteinaz ingibitorom tripsina iz semian grechikhi IT-1.