title: Determination of pyrazinamide in human by high performance liquid chromatography.
abstract: A facile and sensitive high performance liquid chromatographic (HPLC) technique has been developed for the determination pyrazinamide (PZA) in human plasma. Nicotinamide(NIA) is used as internal standard(IS). Plasma is deproteinized with 0.7 M perchloric acid; clear supernatant is neutralized with 1M NaOH and injected onto HPLC. The separation of pyrazinamide and the internal standard is carried out on a Supelco LC-18 (DB) column with a basic mobile phase. Pyrazinoic acid, the major metabolite, other anti-tuberculous drugs and endogenous components do not interfere with measurement of pyrazinamide. The limit of detection of pyrazinamide with this method is 0.2 mg/0.2 ml plasma (CV 8.2%).
authors.affiliation: Medical Department, Hindustan Ciba Geigy Research Centre, Bombay, Maharashtra.
authors.forename: S N
authors.initials: SN
authors.lastname: Revankar
authors.forename: N D
authors.initials: ND
authors.lastname: Desai
authors.forename: A B
authors.initials: AB
authors.lastname: Vaidya
authors.forename: A D
authors.initials: AD
authors.lastname: Bhatt
authors.forename: B
authors.initials: B
authors.lastname: Anjaneyulu
mesh_terms: D000328:Adult; D000995:Antitubercular Agents / Q000097:blood*; D001682:Biological Availability; D002851:Chromatography, High Pressure Liquid / Q000379:methods / Q000592:standards; D006801:Humans; D008297:Male; D009536:Niacinamide / Q000097:blood; D011718:Pyrazinamide / Q000097:blood* / Q000493:pharmacokinetics; D015203:Reproducibility of Results
chemical_list: D000995:Antitubercular Agents; D009536:Niacinamide; D011718:Pyrazinamide