[A new mode of expression for the assessment of capacities of DNA repair by flow cytometry].
Flow cytometry technic was used to study DNA synthesis of Hep G2 cells following mitomycin C and adriblastine treatments during 24 hours. DNA synthesis was expressed by 2 methods: the new expression global DNA synthesis (S+G2)/G1 that considered the cells during scheduled and unscheduled DNA syntheses of S and G2 phases and the cell cycle (Fox program) that evaluated the cells during scheduled DNA synthesis by the terms G1 = 2n, S = 2n+x and G2 = 4n which excluded unscheduled DNA synthesis. The experimental data treated with this new expression led to the determination of threshold concentrations for the two tested compounds where the DNA repair mechanisms were overloaded, leading to cell death. This term was shown to be more accurate to describe the genotoxic action of compounds. Furthermore, these threshold concentrations of DNA damages was found to be linked with significant increase of micronuclei in the micronucleus test.
Laget
M
M
Laboratoire de Biogénotoxicologie et Mutagénèse Environnementale, Faculté de Pharmacie, Marseille, France.
Guiraud-Dauriac
H
H
de Méo
M P
MP
di Giorgio
C
C
Roth
E
E
Duménil
G
G
D000903:Antibiotics, Antineoplastic / Q000494:pharmacology; D006528:Carcinoma, Hepatocellular / Q000235:genetics; D002455:Cell Division / Q000187:drug effects; D004260:DNA Repair / Q000187:drug effects*; D004273:DNA, Neoplasm / Q000187:drug effects*; D003864:Depression, Chemical; D004317:Doxorubicin / Q000494:pharmacology*; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D066298:In Vitro Techniques; D008113:Liver Neoplasms / Q000235:genetics; D016685:Mitomycin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000166:cytology / Q000187:drug effects
D000903:Antibiotics, Antineoplastic; D004273:DNA, Neoplasm; D016685:Mitomycin; D004317:Doxorubicin
Nouveau mode d'expression pour l'évaluation des capacités de réparation de l'ADN par cytométrie en flux.