Hsp90 is required for the activity of a hepatitis B virus reverse transcriptase.
The heat shock protein Hsp90 is known as an essential component of several signal transduction pathways and has now been identified as an essential host factor for hepatitis B virus replication. Hsp90 interacts with the viral reverse transcriptase to facilitate the formation of a ribonucleoprotein (RNP) complex between the polymerase and an RNA ligand. This RNP complex is required early in replication for viral assembly and initiation of DNA synthesis through a protein-priming mechanism. These results thus invoke a role for the Hsp90 pathway in the formation of an RNP.
Hu
J
J
Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, PA 19111, USA.
Seeger
C
C
D000818:Animals; D016227:Benzoquinones; D006528:Carcinoma, Hepatocellular; D002460:Cell Line; D002645:Chickens; D004261:DNA Replication; D004791:Enzyme Inhibitors / Q000494:pharmacology; D018841:HSP90 Heat-Shock Proteins / Q000302:isolation & purification / Q000378:metabolism*; D006515:Hepatitis B virus / Q000201:enzymology* / Q000502:physiology; D007700:Kinetics; D047029:Lactams, Macrocyclic; D008113:Liver Neoplasms; D008957:Models, Genetic; D011809:Quinones / Q000494:pharmacology; D012367:RNA, Viral / Q000378:metabolism*; D012194:RNA-Directed DNA Polymerase / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism; D012261:Ribonucleoproteins / Q000302:isolation & purification / Q000378:metabolism; D015398:Signal Transduction; D014162:Transfection; D014407:Tumor Cells, Cultured; D014779:Virus Replication
D016227:Benzoquinones; D004791:Enzyme Inhibitors; D018841:HSP90 Heat-Shock Proteins; D047029:Lactams, Macrocyclic; D011809:Quinones; D012367:RNA, Viral; D011994:Recombinant Proteins; D012261:Ribonucleoproteins; D012194:RNA-Directed DNA Polymerase; C001277:geldanamycin