A simple imprint-hybridization method for detection of viroids.
An imprint-hybridization method has been designed to simplify the processing of samples during routine viroid indexing. The method requires minimal sample manipulation and has been evaluated for detection of viroids in 11 viroid-host combinations including 4 viroids (CEVd, CSVd, HSVd, ASBVd) and 7 hosts (chrysanthemum, citron, cucumber, Gynura, tomato, peach and avocado). The method is fast and sensitive, and provides additional information on the sites of viroid accumulation.
Romero-Durbán
J
J
Departamento de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias, Moncada (Valencia), Spain.
Cambra
M
M
Duran-Vila
N
N
D017403:In Situ Hybridization / Q000379:methods*; D008567:Membranes, Artificial; D009691:Nucleic Acid Denaturation; D010942:Plant Viruses / Q000235:genetics / Q000302:isolation & purification*; D010944:Plants / Q000821:virology*; D011145:Polyvinyls; D014772:Viroids / Q000235:genetics / Q000302:isolation & purification*; D005465:Fluorocarbon Polymers
D008567:Membranes, Artificial; D011145:Polyvinyls; C024865:polyvinylidene fluoride; D005465:Fluorocarbon Polymers