title: Immunotoxicologic effects of ethylene dibromide in the mouse and their modulation by the estrous cycle.
abstract: Estrous cycle modulation of immunologic sensitivity to ethylene dibromide (EDB) was studied in addition to toxicologic end points. Female B6C3F1 mice were injected intragastrically with 31.25, 62.5, or 125 mg/kg EDB for 5 days a week for 12 weeks. Vaginal smears determined the estrous cycle. At 125 mg/kg there were decreases in hemoglobin and hematocrit and longer estrous cycles (5.5 vs 4.3 days, p = 0.006), and increases in cholesterol, triglycerides, total protein, and albumin. The negative dose response seen for T- and B-cell mitogenesis around metestrus was absent for mice near estrus. The high dose of EDB prolonged intervals between estrus, was immunotoxic and immunosuppressive.
authors.affiliation: Life Sciences Research Department, IIT Research Institute, Chicago, Illinois 60616-3799, USA.
authors.forename: H V
authors.initials: HV
authors.lastname: Ratajczak
authors.forename: P T
authors.initials: PT
authors.lastname: Thomas
authors.forename: J
authors.initials: J
authors.lastname: Gerhart
authors.forename: R B
authors.initials: RB
authors.lastname: Sothern
mesh_terms: D000818:Animals; D001772:Blood Cell Count; D001774:Blood Chemical Analysis; D002273:Carcinogens / Q000633:toxicity*; D004971:Estrus / Q000187:drug effects / Q000276:immunology*; D015946:Ethylene Dibromide / Q000276:immunology* / Q000633:toxicity*; D005260:Female; D008214:Lymphocytes / Q000187:drug effects; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008934:Mitogens / Q000276:immunology; D013154:Spleen / Q000166:cytology / Q000276:immunology; D014626:Vaginal Smears
chemical_list: D002273:Carcinogens; D008934:Mitogens; D015946:Ethylene Dibromide