Possible mechanism of oxygen radical production by human eosinophils mediated by K+ channel activation.
Quinidine hydrochloride, as potent K+ channel blocker, reduced luminol-dependent chemiluminescence products evoked by the addition of the calcium ionophore A23187 to eosinophils from patients with hypereosinophilic syndrome (n = 3) in a concentration-dependent manner (10-5 mM quinidine). A23187 is known to cause increases in intracellular Ca2+ concentrations in eosinophils. Our results indicate that the production of reactive oxygen species by human eosinophils may be affected by Ca(+)-activated K+ channels.
Saito
M
M
Department of Molecular Pharmacology and Biological Chemistry, Northwestern University Medical School, Chicago, IL 60611, USA.
Hisatome
I
I
Nakajima
S
S
Sato
R
R
D000001:Calcimycin / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004804:Eosinophils / Q000378:metabolism*; D005609:Free Radicals / Q000378:metabolism; D006801:Humans; D008163:Luminescent Measurements; D010100:Oxygen / Q000378:metabolism*; D015221:Potassium Channels / Q000187:drug effects*; D011802:Quinidine / Q000494:pharmacology
D005609:Free Radicals; D015221:Potassium Channels; D000001:Calcimycin; D011802:Quinidine; D010100:Oxygen