Regulation of T cell receptor delta gene rearrangement by c-Myb.
Developmental activation of VDJ recombination at the T cell receptor (TCR) delta locus is controlled by an intronic transcriptional enhancer (E delta). Transcriptional activation by E delta is dependent on c-Myb. To determine whether c-Myb plays a role in the activation of TCR-delta gene rearrangement, we compared VDJ recombination in transgenic mice carrying two versions of a human TCR-delta gene minilocus recombination substrate. One includes a wild-type E delta, whereas the other carries an E delta with a mutation that abrogates c-Myb binding. We demonstrate that an intact Myb binding site is necessary for efficient rearrangement of the minilocus substrate, suggesting that c-Myb plays a crucial role in activating VDJ recombination at the endogenous TCR-delta locus.
Hernández-Munain
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C
Department of Immunology, Duke University Medical Center, Durham, North Carolina 27710, USA.
Lauzurica
P
P
Krangel
M S
MS
D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D004268:DNA-Binding Proteins / Q000235:genetics*; D016694:Gene Rearrangement, delta-Chain T-Cell Antigen Receptor; D006801:Humans; D051379:Mice; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D020598:Proto-Oncogene Proteins c-myb; D011995:Recombination, Genetic; D014158:Transcription, Genetic
D004268:DNA-Binding Proteins; D011518:Proto-Oncogene Proteins; D020598:Proto-Oncogene Proteins c-myb