title: [Interaction of DNA with calcium ions by a vibrational spectroscopy].
abstract: DNA complexes with Ca2+ ions formed in films at various relative humidities were studied by IR-spectroscopy for molar concentration ratio of calcium ions and DNA phosphate groups [Ca2+]/[P] = 0.4-20. It is shown that the transition of DNA complexed with Ca2+ ions into B-form occurs at higher average numbers (n) of water molecules absorbed per nucleotide: n > 18 at the ratio [Ca2+]/[P] = 10, n > 24 at [Ca2+]/[P] = 20 and n > 12 in the absence of Ca2+ ions. This shows stronger binding of water molecules in the hydrate shell of DNA-Ca2+ if compared with free DNA. The results confirm a model proposed earlier. Ca2+ ions interact with phosphate oxygens of DNA and with N(7) nitrogens of guanine forming chelated complexes.
authors.forename: S V
authors.initials: SV
authors.lastname: Kornilova
authors.forename: L E
authors.initials: LE
authors.lastname: Kapinos
authors.forename: Iu P
authors.initials: IuP
authors.lastname: Blagoĭ
mesh_terms: D000818:Animals; D002118:Calcium / Q000737:chemistry*; D002412:Cations; D002417:Cattle; D004247:DNA / Q000737:chemistry*; D010100:Oxygen / Q000737:chemistry; D013055:Spectrophotometry, Infrared / Q000379:methods; D014867:Water / Q000737:chemistry
chemical_list: D002412:Cations; D014867:Water; D004247:DNA; D010100:Oxygen; D002118:Calcium
vernacular_title: Izuchenie vzaimodeĭstviia DNK s ionami kal'tsiia metodom kolebatel'noĭ spektroskopii.