Mobilization of annexin V during the uptake of DNP-albumin by human dendritic cells.
Dendritic cells play a crucial role in antigen presentation in various tissues. The endocytic capacity of these cells has been regarded as minimal, but recent work on dendritic cells from mouse spleen has disclosed that the fluid-phase traffic through late endosomes is as active in dendritic cells as in other antigen-presenting cell types. We show that cultured human dendritic cells express the annexins I, III, IV, V and VI, as detected by immunofluorescence staining. The annexins are cytosolic Ca(2+)-dependent proteins with the ability to promote vesicle aggregation and membrane fusion through their capacity to bind to membrane phospholipids. Annexin I and VI appeared to outline the cytoskeleton and the plasma membrane in cultured human dendritic cells. Studies using confocal laser scanning microscopy showed that during the endocytosis of fluorescent dinitrophenyl-conjugated albumin by dendritic cells, there was a redistribution of annexin V which was found to colocalize with vesicles containing dinitrophenyl-FITC-conjugated albumin.
Larsson
M
M
Department of Clinical, Microbiobiology, Faculty of Health Sciences, University of Linköping, Sweden.
Majeed
M
M
Stendahl
O
O
Magnusson
K E
KE
Ernst
J D
JD
Forsum
U
U
D000418:Albumins / Q000378:metabolism*; D017304:Annexin A5 / Q000378:metabolism*; D001692:Biological Transport; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000378:metabolism*; D004705:Endocytosis; D016650:Fluorescein-5-isothiocyanate; D005456:Fluorescent Dyes; D006801:Humans; D018613:Microscopy, Confocal
D000418:Albumins; D017304:Annexin A5; D005456:Fluorescent Dyes; D016650:Fluorescein-5-isothiocyanate