Z-DNA-forming sites within the human beta-globin gene cluster.
Agarose-encapsulated, metabolically active, permeabilized nuclei from human hematopoietic cell lines were tested for Z-DNA formation in the beta-globin gene cluster. Biotinylated monoclonal antibodies against Z-DNA were diffused into the nuclei and cross-linked to DNA with a 10-ns laser exposure at 266 nm. Following digestion with restriction enzymes, fragments that had formed Z-DNA were isolated. Seventeen regions with Z-DNA sequence motifs in the 73-kb region were studied by PCR amplification, and five were found in the Z conformation.
Müller
V
V
Abteilung Molekularbiologie und Bioinformatik, Freie Universität Berlin, Germany.
Takeya
M
M
Brendel
S
S
Wittig
B
B
Rich
A
A
D002478:Cells, Cultured; D004247:DNA / Q000737:chemistry*; D018076:DNA, Complementary; D004339:Drug Compounding; D005914:Globins / Q000235:genetics*; D006412:Hematopoietic Stem Cells / Q000737:chemistry; D006801:Humans; D008969:Molecular Sequence Data; D005810:Multigene Family; D009690:Nucleic Acid Conformation; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA
D018076:DNA, Complementary; D005914:Globins; D004247:DNA