title: Adaptive response of Schizosaccharomyces pombe to hydrogen peroxide and menadione.
abstract: The response of Schizosaccharomyces pombe to oxidative stresses has been examined. On challenging Schiz. pombe for 60 min at early exponential phase with either 40 mM H2O2 or 6 mM menadione (MD), a superoxide-generating agent, less than 10% of the cells survived. Pretreating Schiz. pombe cells with 0.2 mM H2O2 or 0.2 mM MD for 1 h significantly increased survival of these lethal doses of each oxidant, indicating the existence of an adaptive response to oxidative stress. Furthermore, cells pretreated with a low dose of MD became resistant to a lethal dose of H2O2. However, cells pretreated with H2O2 became only partially resistant to a lethal dose of MD. Adaptation was accompanied by the induction of several oxidative defence enzymes. The presence of 0.2 mM H2O2 induced catalase by 2.8-fold and peroxidase by 2.0-fold The presence of 0.2 mM MD induced catalase by 2.0-fold, glucose-6-phosphate dehydrogenase by 1.9-fold, glutathione reductase by 2.7-fold, peroxidase by 3.0-fold, and superoxide dismutase (SOD) by 2.1-fold. The higher induction of these defence enzymes by MD may explain why MD-pretreated cells were better adapted to lethal doses of oxidants than H2O2-pretreated ones. All these enzymes except SOD and peroxidase increased more than 5.0-fold as cells proceeded into stationary phase. The GSH/GSSG ratio also increased by 60%. These changes accord with the observation that stationary phase cells survive oxidant treatment better than cells in vegetative growth.
authors.affiliation: Department of Microbiology, College of Natural Sciences, Seoul National University, Korea.
authors.forename: J
authors.initials: J
authors.lastname: Lee
authors.forename: I W
authors.initials: IW
authors.lastname: Dawes
authors.forename: J H
authors.initials: JH
authors.lastname: Roe
mesh_terms: D000222:Adaptation, Physiological; D000975:Antioxidants / Q000378:metabolism; D002374:Catalase / Q000096:biosynthesis; D004790:Enzyme Induction / Q000187:drug effects; D005954:Glucosephosphate Dehydrogenase / Q000096:biosynthesis; D005978:Glutathione / Q000378:metabolism; D005980:Glutathione Reductase / Q000096:biosynthesis; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D018384:Oxidative Stress / Q000502:physiology; D010544:Peroxidases / Q000096:biosynthesis; D012568:Schizosaccharomyces / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000096:biosynthesis; D014812:Vitamin K / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D014812:Vitamin K; D006861:Hydrogen Peroxide; D005954:Glucosephosphate Dehydrogenase; D010544:Peroxidases; D002374:Catalase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase; D005978:Glutathione