title: Direct detection of disomy in human sperm by the PRINS technique.
abstract: We report the use of the PRimedIN Situ (PRINS) labelling technique for the direct estimation of disomy rates for various chromosomes in human sperm. The PRINS technique provides a rapid and reliable method for chromosome screening since specific labelling may be obtained in less than 2 h. In the present study, the disomy rates of chromosomes 2, 5, 9, 12 and 18 were investigated. The incidences of disomy are similar for all these chromosomes, ranging from 0.27% to 0.33%. These findings suggest an equal distribution of aneuploidies among autosomes in human sperm.
authors.affiliation: CNRS UPR 9008, BP 5051, Montpellier, France.
authors.forename: F
authors.initials: F
authors.lastname: Pellestor
authors.forename: I
authors.initials: I
authors.lastname: Quennesson
authors.forename: L
authors.initials: L
authors.lastname: Coignet
authors.forename: A
authors.initials: A
authors.lastname: Girardet
authors.forename: B
authors.initials: B
authors.lastname: Andréo
authors.forename: J P
authors.initials: JP
authors.lastname: Charlieu
mesh_terms: D000782:Aneuploidy; D001483:Base Sequence; D002874:Chromosome Mapping; D002881:Chromosomes, Human, Pair 12; D002887:Chromosomes, Human, Pair 18; D002889:Chromosomes, Human, Pair 2; D002895:Chromosomes, Human, Pair 5; D002899:Chromosomes, Human, Pair 9; D017931:DNA Primers; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008969:Molecular Sequence Data; D013094:Spermatozoa / Q000166:cytology*
chemical_list: D017931:DNA Primers