Partial structure of the mouse glucokinase gene.
A complementary DNA for glucokinase (GK) was cloned from mouse liver total RNA by a combination of the polymerase chain reaction (PCR) and mouse liver cDNA library screening. Liver- and beta-cell-specific exons 1 were isolated by PCR using mouse and rat genomic DNAs. These clones were then used to screen a mouse genomic library; three genomic clones were isolated and characterized. The mouse GK gene spans over 20 kb, containing 11 exons including a liver- or beta-cell-specific exon 1, which encodes a tissue-specific 15-aa peptide at the N-terminus of the protein. Both types of GK contain 465 amino acid residues. The predicted amino acid sequence of mouse beta-cell-specific GK showed 98 and 96% identity to the rat and human enzymes, respectively; the corresponding values are 98 and 95%, respectively, for the liver-specific GK. Several transcription factor-binding consensus sequences are identified in the 5' flanking region of the mouse GK gene.
Ishimura-Oka
K
K
Department of Cell Biology, Baylor College of Medicine, Houston, Texas 77030, USA.
Nakamuta
M
M
Chu
M J
MJ
Sullivan
M
M
Chan
L
L
Oka
K
K
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D015723:Gene Library; D005941:Glucokinase / Q000235:genetics*; D006801:Humans; D007515:Islets of Langerhans / Q000201:enzymology*; D007527:Isoenzymes / Q000235:genetics; D008099:Liver / Q000201:enzymology*; D051379:Mice / Q000235:genetics*; D008969:Molecular Sequence Data; D009928:Organ Specificity; D016133:Polymerase Chain Reaction; D051381:Rats; D017386:Sequence Homology, Amino Acid
D017931:DNA Primers; D007527:Isoenzymes; D005941:Glucokinase