Flow cytometry analysis of the neutralization effect of anti-IL-8 McAbs on IL-8-activated human granulocytes.
Two hybridoma clones, I8-S2 and I 8-63, secreting anti- IL-8 antibodies were constructed from fusion of immunized spleen cells of BALB/c mice with either Sp 2/0 or 653 myeloma cells. They recognized different epitopes on IL-8 molecule. IL-8 could activate human granulocytes and induce the elevation of [Ca2+]i. Through flow cytometry analysis the two clones displayed different neutralizing effects on the cell activation function of IL-8. The neutralizing effect of clone I8-S2 was apparently higher than that of clone I8-63. It is suggested that the cell activation function of IL-8 is restricted to a certain epitope of IL-8 molecule.
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Shanghai Institute of Cell Biology, Academia Sinica, China.
Ye
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D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000494:pharmacology; D001693:Biological Transport, Active; D002118:Calcium / Q000378:metabolism; D005434:Flow Cytometry; D006098:Granulocytes / Q000378:metabolism*; D006801:Humans; D006825:Hybridomas / Q000378:metabolism; D016209:Interleukin-8 / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C
D000911:Antibodies, Monoclonal; D016209:Interleukin-8; D002118:Calcium