Fluoresein diacetate uptake to determine the viability of human fetal cerebral cortical cells.
We investigated the accuracy of fluorescein diacetate uptake as an indicator of the viability of human fetal cerebral cortical cells. Cortical cells from 16-26-week-old normal fetuses were studied. The cortices were dissociated mechanically with normal saline to make a suspension. Fluorescein diacetate uptake and trypan blue exclusion were compared as methods for examining cell viability. Our results show that fluorescein diacetate uptake is a simple and sensitive method for examining human fetal cortical cell viability.
Zhang
F
F
Department of Neurosurgery, Second Hospital, Henan Medical University, People's Republic of China.
Zhang
P
P
Wang
E
E
Su
F
F
Qi
Y
Y
Bu
X
X
Wang
Y
Y
D002470:Cell Survival / Q000502:physiology; D002540:Cerebral Cortex / Q000166:cytology* / Q000196:embryology / Q000378:metabolism*; D005260:Female; D016332:Fetal Tissue Transplantation; D005452:Fluoresceins / Q000493:pharmacokinetics*; D006801:Humans; D011247:Pregnancy; D012680:Sensitivity and Specificity; D014343:Trypan Blue / Q000493:pharmacokinetics
D005452:Fluoresceins; D014343:Trypan Blue; C018506:diacetylfluorescein