title: Studies of rifamycin production by Amycolatopsis mediterranei cells immobilized on glass wool.
abstract: Cells of Amycolatopsis mediterranei CBS 42575 were immobilized on glass wool for the production of rifamycins B and SV. Glass wool (CORNING type) of 8 microns in diameter has a better entrapment capacity for microbial cells of microorganism than the other types of glass wool used. The most suitable amount of glass wool was 0.8 g/50 ml. The best initial cell concentration used as inoculum was 40 mg cells/50 ml. Repeated batch production of rifamycins by immobilized cells on glass wool was carried out for 6 repeated batches. The results showed that reduction of batch time from 96 h to 48 h does not decrease rifamycin production by immobilized cells.
authors.affiliation: Ain Shams University, Faculty of Science, Microbiology Department, Cairo, Egypt.
authors.forename: M R
authors.initials: MR
authors.lastname: Abu-Shady
authors.forename: A I
authors.initials: AI
authors.lastname: el-Diwany
authors.forename: M A
authors.initials: MA
authors.lastname: Farid
authors.forename: H A
authors.initials: HA
authors.lastname: el-Enshasy
mesh_terms: D039903:Actinobacteria / Q000378:metabolism*; D018914:Cells, Immobilized / Q000378:metabolism*; D003470:Culture Media; D005285:Fermentation; D005898:Glass; D012294:Rifamycins / Q000096:biosynthesis*
chemical_list: D003470:Culture Media; D012294:Rifamycins; C033974:rifamycin B; C023808:rifamycin SV