Tubulin aggregates induced by Ni2+ present microtubular characteristics.
Rat brain tubulin in a proper buffered solution became insoluble in the presence of 10 mM NiCl2, and sedimented at centrifugal forces as low as 500 x g for 30 min. Both nickel-sedimented and microtubular tubulin conserved 65% of colchicine binding activity after 25 days of storage at -20 degrees C. However in brain cytosol, only 9% of the initial binding activity was conserved. The electrophoretic mobility of tubulin recovered from aggregates also remained unaltered. Therefore the aggregates formed with Ni2+ share important physicochemical properties with microtubules.
Berón
W
W
Instituto de Histología y Embriología (IHEM), Facultad de Ciencias Médicas, Universidad Nacional de Cuyo, Mendoza, Argentina.
López
L
L
Bertini
F
F
D000818:Animals; D001923:Brain Chemistry; D002498:Centrifugation; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D003078:Colchicine / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D008297:Male; D008870:Microtubules / Q000737:chemistry*; D009532:Nickel / Q000494:pharmacology*; D051381:Rats; D012995:Solubility; D014404:Tubulin / Q000737:chemistry* / Q000378:metabolism
D014404:Tubulin; C022838:nickel chloride; D009532:Nickel; D003078:Colchicine