title: Crossreactivity between Dermatophagoides siboney and other house dust mite allergens in sensitized asthmatic patients.
abstract: BACKGROUND
  The role of Pyroglyphid mites in IgE-mediated sensitization has been well established. Although mites belonging to the genus Dermatophagoides dominate in the acarofauna of domestic dust, non-pyroglyphid mites might also be of clinical importance. In Cuba, Dermatophagoides siboney is found in dust coexisting with D. pteronyssinus and Blomia tropicalis. Dermatophagoides farinae is not found. Storage mites, such as Acarus siro, Lepidoglyphus destructor and Tyrophagus putrescentiae, might also be present.
  
  
  OBJECTIVE
  The aim of this study was to investigate the allergenic relationships among the mites present in the environment of allergic patients in Cuba.
  
  
  METHODS
  The crossreactivity between an extract of D. siboney and the above mentioned six mites was studied by specific IgE inhibition, using sera of mite-sensitive patients after incubation with serial dilutions of D. siboney.
  
  
  RESULTS
  The inhibitory effect of D. siboney extract was more pronounced against D. farinae, 91%, than against D. pteronyssinus, 62%. Specific IgE to B. tropicalis, A. siro, L. destructor and T. putrescentiae were inhibited to a much lesser extent, i.e. between 47 and 58%. The correlation between IgE to D. siboney and IgE to D. pteronyssinus and D. farinae was of 0.92 and 0.90, respectively. A weaker correlation was found between D. siboney and L. destructor (0.7), A. siro (0.67), B. tropicalis (0.54) and T. putrescentiae (0.51).
  
  
  CONCLUSIONS
  Thus, D. siboney seems to be most closely related to D. farinae.
authors.affiliation: Department of Allergens, National Center for Bioproducts, Havana, Cuba.
authors.forename: R
authors.initials: R
authors.lastname: Ferrándiz
authors.forename: R
authors.initials: R
authors.lastname: Casas
authors.forename: S
authors.initials: S
authors.lastname: Dreborg
authors.forename: R
authors.initials: R
authors.lastname: Einarsson
authors.forename: B
authors.initials: B
authors.lastname: Fernández
mesh_terms: D000293:Adolescent; D000328:Adult; D000485:Allergens / Q000097:blood / Q000276:immunology / Q000494:pharmacology; D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000494:pharmacology; D000918:Antibody Specificity; D039741:Antigens, Dermatophagoides; D001249:Asthma / Q000097:blood / Q000276:immunology*; D001667:Binding, Competitive / Q000276:immunology; D003429:Cross Reactions; D004391:Dust / Q000009:adverse effects*; D005260:Female; D006023:Glycoproteins / Q000097:blood / Q000276:immunology* / Q000494:pharmacology; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000097:blood / Q000276:immunology*; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology; D008297:Male; D008875:Middle Aged; D008925:Mites / Q000276:immunology*
chemical_list: D000485:Allergens; D000888:Antibodies, Anti-Idiotypic; D039741:Antigens, Dermatophagoides; D004391:Dust; D006023:Glycoproteins; C026408:anti-IgE antibodies; D007073:Immunoglobulin E