Integrative and replicative transformation of Penicillium canescens with a heterologous nitrate-reductase gene.
A wild isolate of Penicillium canescens was subjected to mutagenesis, and 150 chlorate-resistant mutants were isolated and classified in respect of their ability to utilize various nitrogen sources. Strains supposedly deficient in nitrate reductase have been transformed with the nitrate-reductase gene from Aspergillus niger. Transformation probably occurred by non-homologous integration of the transforming vector into the chromosome. Co-transformation with the AMA 1 replicating element from A. nidulans enhanced transformation frequency up to 2000-fold, and was shown to result in autonomous maintenance of replicating concatenates, one of which was re-isolated by transformation of E. coli.
Aleksenko
A Y
AY
Genetics Laboratories, University of Glasgow, Scotland, UK.
Makarova
N A
NA
Nikolaev
I V
IV
Clutterbuck
A J
AJ
D004271:DNA, Fungal; D016296:Mutagenesis; D050901:Nitrate Reductase; D009565:Nitrate Reductases / Q000235:genetics*; D009584:Nitrogen / Q000378:metabolism; D010407:Penicillium / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D014170:Transformation, Genetic
D004271:DNA, Fungal; D009565:Nitrate Reductases; D050901:Nitrate Reductase; D009584:Nitrogen