Identification of a putative target for Rho as the serine-threonine kinase protein kinase N.
Rho, a Ras-like small guanosine triphosphatase, has been implicated in cytoskeletal responses to extracellular signals such as lysophosphatidic acid (LPA) to form stress fibers and focal contacts. The form of RhoA bound to guanosine triphosphate directly bound to and activated a serine-threonine kinase, protein kinase N (PKN). Activated RhoA formed a complex with PKN and activated it in COS-7 cells. PKN was phosphorylated in Swiss 3T3 cells stimulated with LPA, and this phosphorylation was blocked by treatment of cells with botulinum C3 exoenzyme. Activation of Rho may be linked directly to a serine-threonine kinase pathway.
Amano
M
M
Division of Signal Transduction, Nara Institute of Science and Technology, Ikoma, Japan.
Mukai
H
H
Ono
Y
Y
Chihara
K
K
Matsui
T
T
Hamajima
Y
Y
Okawa
K
K
Iwamatsu
A
A
Kaibuchi
K
K
D016475:3T3 Cells; D036002:ADP Ribose Transferases / Q000494:pharmacology; D000595:Amino Acid Sequence; D000818:Animals; D001905:Botulinum Toxins; D002460:Cell Line; D002846:Chromatography, Affinity; D004789:Enzyme Activation; D020558:GTP Phosphohydrolases / Q000378:metabolism*; D019204:GTP-Binding Proteins / Q000378:metabolism*; D006160:Guanosine Triphosphate / Q000378:metabolism; D008246:Lysophospholipids / Q000494:pharmacology; D051379:Mice; D008969:Molecular Sequence Data; D010766:Phosphorylation; D011493:Protein Kinase C / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D020742:rhoA GTP-Binding Protein
D008246:Lysophospholipids; D011993:Recombinant Fusion Proteins; D006160:Guanosine Triphosphate; D036002:ADP Ribose Transferases; C063007:exoenzyme C3, Clostridium botulinum; C061570:protein kinase N; D011493:Protein Kinase C; D001905:Botulinum Toxins; D020558:GTP Phosphohydrolases; D019204:GTP-Binding Proteins; D020742:rhoA GTP-Binding Protein