Effect of hematopoietic growth factors on human blood monocytes/macrophages in in vitro culture.
The production of mature monocytes/macrophages is regulated by a group of hematopoietic growth factors, or colony-stimulating factors (CSF). We investigated the in vitro effect of human hematopoietic growth factors on human blood monocyte/macrophage differentiation and proliferation in short- and long-term in vitro cultures. The addition of macrophage CSF, granulocyte-macrophage CSF, and granulocyte CSF and interleukin-6 and interleukin-3 growth factors to monocyte/macrophage cultures induced morphological changes in cultured cells, including enhancement of cell growth and the formation of multinucleated giant cells, spindle-like cells, and fibroblast-like cells. In addition, CD4 and HLA-DR antigen expression was down regulated by the addition of growth factors without a change in the expression of other surface antigens, including CD3, CD11B, CD14, CD15, NK H1, and B1. The proliferating cell nuclear antigen was not detected in growth factor-treated nonadherent monocytes/macrophages in long-term cultures. Bromodeoxyuridine was incorporated in the adherent monocytes/macrophages, and intense staining in the small rounded cells which occur above the adherent cells in these cultures was observed after a 72-h pulse, indicating that monocytes/macrophages are slowly dividing cells.
Hassan
N F
NF
Division of Allergy, Immunology, and Infectious Diseases, Children's Hospital of Philadelphia, University of Pennsylvania School of Medicine, USA.
Chehimi
J
J
Ho
W Z
WZ
Campbell
D E
DE
Douglas
S D
SD
D015703:Antigens, CD / Q000276:immunology; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D002455:Cell Division / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured / Q000276:immunology; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000276:immunology / Q000494:pharmacology; D016298:Hematopoietic Cell Growth Factors / Q000276:immunology* / Q000494:pharmacology; D006801:Humans; D007377:Interleukin-3 / Q000276:immunology / Q000494:pharmacology; D016173:Macrophage Colony-Stimulating Factor / Q000276:immunology / Q000494:pharmacology; D008264:Macrophages / Q000166:cytology* / Q000648:ultrastructure; D009000:Monocytes / Q000166:cytology* / Q000648:ultrastructure; D011956:Receptors, Cell Surface / Q000276:immunology; D013997:Time Factors
D015703:Antigens, CD; D016298:Hematopoietic Cell Growth Factors; D007377:Interleukin-3; D011956:Receptors, Cell Surface; D016173:Macrophage Colony-Stimulating Factor; D016178:Granulocyte-Macrophage Colony-Stimulating Factor