title: Methods and reagents. Quantitative PCR: an accurate measure of mRNA?
abstract: Methods and reagents is a unique monthly column that highlights current discussions in the newsgroup bionet.molbio.methds-reagnts, available on the Internet. This month's column discusses quantitative measurements of messenger RNA levels by using reverse transcription polymerase chain reaction (RT-PCR). For details on how to partake in the newsgroup, see the accompanying box.
authors.affiliation: National Cancer Institute, Frederick Cancer Research and Development Center, MD 21702-1201, USA.
authors.forename: P N
authors.initials: PN
authors.lastname: Hengen
mesh_terms: D015152:Blotting, Northern; D017931:DNA Primers; D018076:DNA, Complementary / Q000378:metabolism; D015870:Gene Expression; D016133:Polymerase Chain Reaction / Q000379:methods*; D012333:RNA, Messenger / Q000032:analysis*; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D012260:Ribonucleases / Q000378:metabolism
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D012333:RNA, Messenger; D012194:RNA-Directed DNA Polymerase; D012260:Ribonucleases