title: The murine homolog of the human breast and ovarian cancer susceptibility gene Brca1 maps to mouse chromosome 11D.
abstract: The recently cloned human breast and ovarian cancer susceptibility gene, BRCA1, is located on human chromosome 17q21. We have isolated murine genomic clones containing Brca1 as a first step in generating a mouse model for the loss of BRCA1 function. A mouse genomic library was screened using probes corresponding to exon 11 of the human BRCA1 gene. Two overlapping mouse clones were identified that hybridized to human BRCA1 exons 9-12. Sequence analysis of 1.4 kb of the region of these clones corresponding to part of human exon 11 revealed 72% nucleic acid identity but only 50% amino acid identity with the human gene. The longest of the mouse Brca1 genomic clones maps to chromosome 11D, as determined by two-color fluorescence in situ hybridization. The synteny to human chromosome 17 was confirmed by cohybridization with the mouse probe for the NF1-gene. This comparative study confirms that the relative location of the BRCA1 gene has been conserved between mice and humans.
authors.affiliation: Diagnostic Development Branch, National Center for Human Genome Research/NIH, Bethesda, MD 20892-4470, USA.
authors.forename: E
authors.initials: E
authors.lastname: Schröck
authors.forename: P
authors.initials: P
authors.lastname: Badger
authors.forename: D
authors.initials: D
authors.lastname: Larson
authors.forename: M
authors.initials: M
authors.lastname: Erdos
authors.forename: A
authors.initials: A
authors.lastname: Wynshaw-Boris
authors.forename: T
authors.initials: T
authors.lastname: Ried
authors.forename: L
authors.initials: L
authors.lastname: Brody
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D019313:BRCA1 Protein; D001943:Breast Neoplasms / Q000235:genetics*; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000235:genetics*; D010051:Ovarian Neoplasms / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D019313:BRCA1 Protein; D009363:Neoplasm Proteins; D014157:Transcription Factors