title: Fluoresein diacetate uptake to determine the viability of human fetal cerebral cortical cells.
abstract: We investigated the accuracy of fluorescein diacetate uptake as an indicator of the viability of human fetal cerebral cortical cells. Cortical cells from 16-26-week-old normal fetuses were studied. The cortices were dissociated mechanically with normal saline to make a suspension. Fluorescein diacetate uptake and trypan blue exclusion were compared as methods for examining cell viability. Our results show that fluorescein diacetate uptake is a simple and sensitive method for examining human fetal cortical cell viability.
authors.affiliation: Department of Neurosurgery, Second Hospital, Henan Medical University, People's Republic of China.
authors.forename: F
authors.initials: F
authors.lastname: Zhang
authors.forename: P
authors.initials: P
authors.lastname: Zhang
authors.forename: E
authors.initials: E
authors.lastname: Wang
authors.forename: F
authors.initials: F
authors.lastname: Su
authors.forename: Y
authors.initials: Y
authors.lastname: Qi
authors.forename: X
authors.initials: X
authors.lastname: Bu
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
mesh_terms: D002470:Cell Survival / Q000502:physiology; D002540:Cerebral Cortex / Q000166:cytology* / Q000196:embryology / Q000378:metabolism*; D005260:Female; D016332:Fetal Tissue Transplantation; D005452:Fluoresceins / Q000493:pharmacokinetics*; D006801:Humans; D011247:Pregnancy; D012680:Sensitivity and Specificity; D014343:Trypan Blue / Q000493:pharmacokinetics
chemical_list: D005452:Fluoresceins; D014343:Trypan Blue; C018506:diacetylfluorescein