Comparison of outer membrane protein and lipopolysaccharide profiles of enterotoxigenic Escherichia coli strains isolated in São Paulo, Brazil.
The outer membrane protein (OMP) and lipopolysaccharide (LPS) patterns of 12 strains of serogroups of enterotoxigenic E. coli frequently isolated in São Paulo city were determined by fractionation techniques and by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Five O6, three O78 and four O128 serogroup isolates of different serotypes (flagellar antigens) and virulence factors (toxins and colonization factor antigens) showed a high degree of variability in their OMP pattern and at least nine groups could be identified. The analysis of LPS patterns by SDS-PAGE showed a homogeneous profile for the O6 strains and some minor differences for the O128 and O78 strains. The present data indicate that analysis of OMP and LPS by SDS-PAGE may further improve the discriminating ability of extensively used serological techniques or the detection of virulence factors and could be a useful tool in epidemiological studies of enterotoxigenic E. coli (ETEC) strains from this area.
Guth
B E
BE
Disciplina de Microbiologia e Imunologia, Escola Paulista de Medicina, São Paulo, Brasil.
Pacheco
A B
AB
von Krüger
W M
WM
Ferreira
L C
LC
D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry* / Q000302:isolation & purification; D001938:Brazil; D004926:Escherichia coli / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000302:isolation & purification
D001425:Bacterial Outer Membrane Proteins; D008070:Lipopolysaccharides