Regulation of BPAG2 (180 kDa) gene expression in spontaneously transformed murine keratinocytes: tissue distribution, modulation during cell adhesion, and up-regulation by ascorbic acid.
Messenger RNA for BPAG2, a hemidesmosomal type XVII collagen, was detected specifically in cultured keratinocytes, not in melanocytes or dermal fibroblasts, and was localized preferentially in the skin and cornea, not in aorta, lung, kidney, or liver. Modulation of BPAG2 mRNA expression during keratinocyte adhesion and growth in culture was studied. BPAG2 mRNA level in the spreading phase was greater than that in cell attaching and proliferating phases. Ascorbic acid stimulated the BPAG2 mRNA level to 2 times the control in a dose- and exposure time-dependent manner. These results suggest that BPAG2 is expressed at a maximum level in the cell spreading phase and is up-regulated by ascorbic acid.
Tajima
S
S
Department of Dermatology, Keio University School of Medicine, Tokyo.
Tokimitsu
I
I
D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology*; D001483:Base Sequence; D002448:Cell Adhesion; D002455:Cell Division; D002461:Cell Line, Transformed; D003094:Collagen / Q000096:biosynthesis* / Q000235:genetics; D015603:Keratinocytes / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D008969:Molecular Sequence Data; D009928:Organ Specificity; D012333:RNA, Messenger / Q000032:analysis; D015854:Up-Regulation
D012333:RNA, Messenger; D003094:Collagen; D001205:Ascorbic Acid