Immunodiagnosis of dracunculiasis by dot-ELISA.
A dot-enzyme-linked immunosorbent assay (dot-ELISA) using soluble adult-guineaworm antigen was developed for immunodiagnosis of Dracunculus medinensis infection. The test was found to be specific and sensitive when compared with ELISA. The dot-ELISA described here was found to be time-saving and easy to perform, and its possible use as a diagnostic and epidemiological tool under field conditions is therefore discussed.
Prakash
D
D
National Chemical Laboratory, Pune, India.
Parab
P B
PB
Sharma
R N
RN
D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology; D004320:Dracunculiasis / Q000175:diagnosis*; D004321:Dracunculus Nematode / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D012698:Serologic Tests
D000947:Antigens, Helminth