title: Immune response and protection against influenza A infection in mice immunised with subunit influenza A vaccine in combination with whole cell or acellular DTP vaccine.
abstract: Following the demonstration of the strong adjuvant effect of whole-cell DTP vaccine (wDTP) on the immune responses to influenza subunit vaccine, studies were undertaken to identify the component of wDTP responsible for the adjuvant effect, and to determine if acellular DTP (aDTP) vaccine was as effective since it is less reactogenic and likely to replace wDTP for primary or secondary immunisation. In addition, wDTP and aDTP were directly compared in a dose-response study. Experiments in mice indicated that the adjuvant effect of wDTP resided in the LPS component of B. pertussis, since purified LPS enhanced the IgG antibody response, the IgG subclass response and protection to the same level as wDTP. An adjuvant effect was detected using aDTP, but was statistically less pronounced than wDTP by a factor of some 100-fold. These results suggest that immunisation against influenza in infants and young children can be achieved combining small amounts of influenza antigen with wDTP or LPS, and to a lesser extent by combining vaccine with aDTP. However, these results were obtained in mice and should be confirmed in man since species vary considerably in response to adjuvant.
authors.affiliation: Department of Medical Microbiology, University of Sheffield Medical School, UK.
authors.forename: H
authors.initials: H
authors.lastname: Tamizifar
authors.forename: A
authors.initials: A
authors.lastname: Robinson
authors.forename: R
authors.initials: R
authors.lastname: Jennings
authors.forename: C W
authors.initials: CW
authors.lastname: Potter
mesh_terms: D000276:Adjuvants, Immunologic / Q000627:therapeutic use; D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis* / Q000097:blood; D015721:Diphtheria-Tetanus-Pertussis Vaccine / Q000627:therapeutic use*; D004305:Dose-Response Relationship, Drug; D004359:Drug Therapy, Combination; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000097:blood / Q000145:classification; D009980:Influenza A virus / Q000276:immunology*; D007252:Influenza Vaccines / Q000627:therapeutic use*; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D009976:Orthomyxoviridae Infections / Q000517:prevention & control*
chemical_list: D000276:Adjuvants, Immunologic; D000914:Antibodies, Viral; D015721:Diphtheria-Tetanus-Pertussis Vaccine; D007074:Immunoglobulin G; D007252:Influenza Vaccines; D008070:Lipopolysaccharides