Tenidap, but not nonsteroidal anti-inflammatory drugs, inhibits T-cell proliferation and cytokine induction.
T-lymphocytes are involved in the inflammatory response that occurs in affected joints of patients with rheumatoid arthritis (RA). Some second-line disease modifying anti-rheumatic drugs used in the treatment of patients with RA are known to block T-cell activation. The present study assessed whether tenidap, an investigational anti-rheumatic drug, affects in vitro T-cell responses such as proliferation and cytokine production. It was found that tenidap, in contrast to several nonsteroidal anti-inflammatory drugs, inhibits anti-CD3 or IL-2 driven proliferative responses of cloned human T-cells. Furthermore, tenidap was found to inhibit IFN-gamma production as well as the induction of mRNA encoding IFN-gamma or TNF-alpha. The results indicate that tenidap may exert at least part of its anti-inflammatory activity via inhibition of T-cell function and cytokine production.
Dolhain
R J
RJ
Department of Rheumatology, University Hospital Leiden, The Netherlands.
de Kuiper
P
P
Verweij
C L
CL
Penders
J M
JM
Breedveld
F C
FC
Dijkmans
B A
BA
Miltenburg
A M
AM
D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D001172:Arthritis, Rheumatoid / Q000188:drug therapy / Q000276:immunology*; D001483:Base Sequence; D017252:CD3 Complex / Q000276:immunology; D002455:Cell Division / Q000187:drug effects; D002999:Clone Cells; D016207:Cytokines / Q000096:biosynthesis*; D017931:DNA Primers; D006801:Humans; D007211:Indoles / Q000494:pharmacology*; D007376:Interleukin-2 / Q000276:immunology; D008213:Lymphocyte Activation; D008969:Molecular Sequence Data; D000078183:Oxindoles; D012333:RNA, Messenger / Q000378:metabolism; D018417:Th1 Cells / Q000187:drug effects* / Q000276:immunology
D000894:Anti-Inflammatory Agents, Non-Steroidal; D017252:CD3 Complex; D016207:Cytokines; D017931:DNA Primers; D007211:Indoles; D007376:Interleukin-2; D000078183:Oxindoles; D012333:RNA, Messenger; C062866:tenidap