title: Identification of five Rhodobacter capsulatus genes encoding the equivalent of ND subunits of the mitochondrial NADH-ubiquinone oxidoreductase.
abstract: We previously reported the sequencing of two genes (ndhA and ndhI) encoding two of the subunits of the type-I NADH-ubiquinone oxidoreductase from Rhodobacter capsulatus (Rc). The present paper deals with the cloning and characterization of a chromosomal fragment clustering five new Rc genes which encode subunits of this enzyme. This gene cluster is located immediately downstream from ndhA and ndhI, and also contains two unidentified open reading frames (urf2, urf3). The five genes, nuoJ, nuoK, nuoL, nuoM and nuoN, encode proteins related, respectively, to mitochondrial (mt) subunits ND6, ND4L, ND5, ND4 and ND2. The overall organization of the nuo genes identified in Rc shows similarity to that of the Paracoccus denitrificans (Pd) nqo gene cluster.
authors.affiliation: Laboratoire de Biochimie, URA CNRS 1130, DBMS, CEA, Grenoble, France.
authors.forename: A
authors.initials: A
authors.lastname: Dupuis
authors.forename: A
authors.initials: A
authors.lastname: Peinnequin
authors.forename: M
authors.initials: M
authors.lastname: Chevallet
authors.forename: J
authors.initials: J
authors.lastname: Lunardi
authors.forename: E
authors.initials: E
authors.lastname: Darrouzet
authors.forename: B
authors.initials: B
authors.lastname: Pierrard
authors.forename: V
authors.initials: V
authors.lastname: Procaccio
authors.forename: J P
authors.initials: JP
authors.lastname: Issartel
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D042967:Electron Transport Complex I; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009247:NADH, NADPH Oxidoreductases / Q000235:genetics*; D009876:Operon; D015183:Restriction Mapping; D016354:Rhodobacter capsulatus / Q000201:enzymology / Q000235:genetics*; D009626:Terminology as Topic
chemical_list: D009247:NADH, NADPH Oxidoreductases; D042967:Electron Transport Complex I