The role of the renin-angiotensin system in the control of blood pressure and drinking in the European eel, Anguilla anguilla.
The role of the renin-angiotensin system (RAS) in the control of blood pressure and drinking was investigated in fresh water (FW)- and seawater (SW)-adapted eels, Anguilla anguilla, by comparing the effects of pharmacological manipulation through the use of papaverine (stimulator) and captopril (inhibitor) on the endogenous system. In SW eels basal blood pressure levels were lower (23.3 +/- 0.8 mm Hg) with correspondingly higher basal drinking rates (0.51 +/- 0.07 ml/kg/hr) and plasma AII concentrations (32.89 +/- 4.19 fmol/ml) compared to FW eels (33.8 +/- 1.3 mm Hg, 0.06 +/- 0.02 ml/kg/hr, 9.72 +/- 0.60 fmol/ml, respectively). In FW eels papaverine caused immediate hypotension with full recovery, decrease in plasma osmolality, and increase in drinking rate and plasma AII concentration, but in SW eels, hypotension with full recovery and an increase in plasma osmolality, drinking rate, and plasma AII concentration occurred. In FW eels captopril had no effect on the parameters measured, but in SW eels it caused a sustained decrease in blood pressure and a decline in the basal drinking rate and plasma AII concentration. Papaverine was also administered 15 min after captopril. In FW eels this manipulation caused hypotension only after the papaverine injection, followed by a partial recovery. Osmolality was unaffected, the previously observed papaverine-induced dipsogenic response was blocked, and the rise in plasma AII concentrations was smaller than with papaverine only. In SW eels there was an immediate hypotension after captopril administration with full recovery.(ABSTRACT TRUNCATED AT 250 WORDS)
Tierney
M L
ML
Gatty Marine Laboratory, School of Biological and Medical Sciences, University of St. Andrews, Fife, United Kingdom.
Luke
G
G
Cramb
G
G
Hazon
N
N
D000804:Angiotensin II / Q000097:blood; D000818:Animals; D000959:Antihypertensive Agents / Q000494:pharmacology; D001794:Blood Pressure / Q000187:drug effects / Q000502:physiology*; D002216:Captopril / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004327:Drinking Behavior / Q000502:physiology*; D004524:Eels / Q000502:physiology*; D005618:Fresh Water; D009994:Osmolar Concentration; D010208:Papaverine / Q000494:pharmacology; D011863:Radioimmunoassay; D012084:Renin-Angiotensin System / Q000502:physiology*; D012623:Seawater; D013997:Time Factors; D014665:Vasodilator Agents / Q000494:pharmacology
D000959:Antihypertensive Agents; D014665:Vasodilator Agents; D000804:Angiotensin II; D002216:Captopril; D010208:Papaverine