A vanadate hematoxylin stain for basic proteins.
Ammonium vanadate can act as both an oxidant and a mordant for hematoxylin. Lithium carbonate can remove vanadate hematoxylin from other structures so that only the most basic proteins are stained. Brief diazotization of the tissue sections restricts staining to the histone proteins of the nucleus.
Smith
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School of Podiatric Medicine, Barry University, Miami Shores, Florida 33161, USA.
D000818:Animals; D006224:Cricetinae; D006416:Hematoxylin; D009696:Nucleic Acids; D016877:Oxidants; D011506:Proteins / Q000032:analysis*; D013194:Staining and Labeling / Q000379:methods*; D016707:Tissue Fixation; D014638:Vanadates
D009696:Nucleic Acids; D016877:Oxidants; D011506:Proteins; D014638:Vanadates; D006416:Hematoxylin