Interleukin 1 binding to its type I, but not type II receptor, modulates the in vivo acute phase response.
Neutralizing monoclonal antibodies against the murine interleukin 1 (IL-1) type I (mAb 35F5) and type II receptor (mAb 4E2) were used to passively immunize mice prior to exogenous murine IL-1 alpha administration or a sterile-turpentine induced abscess. When mice were passively immunized with 35F5, the anorexia, weight loss and increased plasma acute phase protein levels in response to exogenous IL-1 alpha administration or a turpentine abscess were significantly attenuated. In contrast, passive immunization with 4E2 had only variable effects on food intake, body weight and the hepatic acute phase response in mice administered IL-1 alpha. In mice following a turpentine abscess, type II receptor blockade (4E2) either had no effect, or in some cases, actually increased the plasma IL-6 and acute phase protein responses. We conclude that in response to a turpentine abscess, the anorexia, weight loss and the induction of several hepatic acute phase reactants result in part from IL-1 binding to its type I receptor. Binding of IL-1 to the type II IL-1 receptor does not appear to be involved in the induction of these host nonspecific responses to inflammation.
Oldenburg
H S
HS
Department of Surgery, Cornell University Medical College, New York, New York 10021, USA.
Pruitt
J H
JH
Lazarus
D D
DD
Rogy
M A
MA
Chizzonite
R
R
Lowry
S F
SF
Moldawer
L L
LL
D000038:Abscess / Q000139:chemically induced / Q000276:immunology / Q000378:metabolism; D000210:Acute-Phase Reaction / Q000276:immunology / Q000378:metabolism*; D000818:Animals; D000855:Anorexia / Q000517:prevention & control; D000911:Antibodies, Monoclonal; D002478:Cells, Cultured; D007116:Immunization, Passive; D007375:Interleukin-1 / Q000378:metabolism* / Q000494:pharmacology; D051379:Mice; D008810:Mice, Inbred C57BL; D017472:Receptors, Interleukin-1 / Q000378:metabolism*; D014425:Turpentine; D015431:Weight Loss / Q000187:drug effects
D000911:Antibodies, Monoclonal; D007375:Interleukin-1; D017472:Receptors, Interleukin-1; D014425:Turpentine