Nuclear matrix acceptor binding sites for steroid hormone receptors: a candidate nuclear matrix acceptor protein.
Steroid/nuclear-hormone receptors are ligand-activated transcription factors that have been localized to the nuclear matrix. The classic model of hormone action suggests that, following activation, these receptors bind to specific "steroid response elements" on the DNA, then interact with other factors in the transcription initiation complex. However, evidence demonstrates the existence of specific chromatin proteins that act as accessory factors by facilitating the binding of the steroid receptors to the DNA. One such protein, the "receptor binding factor (RBF)-1", has been purified and shown to confer specific, high-affinity binding of the progesterone receptor to the DNA. Interestingly, the RBF-1 is localized to the nuclear matrix. Further, the RBF-1 binds specifically to a sequence of the c-myc proto-oncogene that has the appearance of a nuclear matrix attached region (MAR). These results, and other findings reviewed here, suggest that the nuclear matrix is involved intimately in steroid hormone-regulated gene expression.
Lauber
A H
AH
Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, Minnesota 55904, USA.
Sandhu
N P
NP
Schuchard
M
M
Subramaniam
M
M
Spelsberg
T C
TC
D000818:Animals; D030161:Avian Proteins; D001665:Binding Sites; D002352:Carrier Proteins / Q000032:analysis / Q000378:metabolism*; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000032:analysis / Q000378:metabolism*; D005786:Gene Expression Regulation; D016259:Genes, myc; D015530:Nuclear Matrix / Q000737:chemistry* / Q000378:metabolism; D009687:Nuclear Proteins / Q000378:metabolism*; D011980:Receptors, Progesterone / Q000378:metabolism; D011987:Receptors, Steroid / Q000378:metabolism*
D030161:Avian Proteins; D002352:Carrier Proteins; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; C085646:RBF-1 protein, Gallus gallus; D011980:Receptors, Progesterone; D011987:Receptors, Steroid; D004247:DNA