Effect of magnetic fields on rodent monooxygenase enzymes.
The effects of 50 Hz, 1.2 mT magnetic fields (MFs) were tested on hepatic monooxygenase enzymes of basal and beta-naphthoflavone-phenobarbital-preinduced rats and mice. An inductive effect on cytochrome P-450 level and on some enzymatic cytochrome P-450-dependent activities was observed in basal mice after MF exposure. Enzymatic activities in preinduced mice and rats were reduced by MFs, the degree of reduction depending on the enzyme. A specific inhibitory effect was determined in some of the assayed activities and in the relative peculiar P-450 isoforms detected by Western blot analysis.
Del Carratore
R
R
Istituto di Mutagenesi e Differenziamento CNR, Pisa, Italy.
Morichetti
E
E
Della Croce
C
C
Bronzetti
G
G
D015660:7-Alkoxycoumarin O-Dealkylase / Q000187:drug effects / Q000528:radiation effects; D000815:Aniline Hydroxylase / Q000187:drug effects / Q000528:radiation effects; D000818:Animals; D001571:Benzoflavones / Q000494:pharmacology; D015153:Blotting, Western; D019362:Cytochrome P-450 CYP2B1; D019392:Cytochrome P-450 CYP2E1; D003577:Cytochrome P-450 Enzyme System / Q000187:drug effects / Q000528:radiation effects; D004574:Electromagnetic Fields; D007527:Isoenzymes / Q000187:drug effects / Q000528:radiation effects; D008099:Liver / Q000187:drug effects / Q000201:enzymology* / Q000528:radiation effects; D008280:Magnetics; D051379:Mice; D008861:Microsomes / Q000187:drug effects / Q000201:enzymology / Q000528:radiation effects; D010088:Oxidoreductases / Q000187:drug effects / Q000528:radiation effects*; D010089:Oxidoreductases, N-Demethylating / Q000187:drug effects / Q000528:radiation effects; D010090:Oxidoreductases, O-Demethylating / Q000187:drug effects / Q000528:radiation effects; D010634:Phenobarbital / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D019324:beta-Naphthoflavone
D001571:Benzoflavones; D007527:Isoenzymes; D019324:beta-Naphthoflavone; D003577:Cytochrome P-450 Enzyme System; C026048:4-nitroanisole O-demethylase; D010088:Oxidoreductases; D010090:Oxidoreductases, O-Demethylating; D015660:7-Alkoxycoumarin O-Dealkylase; D019392:Cytochrome P-450 CYP2E1; D000815:Aniline Hydroxylase; D019362:Cytochrome P-450 CYP2B1; D010089:Oxidoreductases, N-Demethylating; D010634:Phenobarbital