Aldosterone inhibits nitric oxide synthesis in rat vascular smooth muscle cells induced by interleukin-1 beta.
We investigated the effects of aldosterone on nitric oxide (NO) synthesis in vascular smooth muscle cells. We measured the production of nitrite, a stable metabolite of NO, and the expression of inducible NO synthase mRNA and protein in cultured rat vascular smooth muscle cells. Incubation of the cultures with interleukin-1 beta (10 ng/ml) for 24 h caused a significant increase in nitrite generation. The interleukin-1 beta-induced nitrite production by vascular smooth muscle cells was significantly inhibited by aldosterone in a dose (10(-9) approximately 10(-6) M)-dependent manner. Incubation with interleukin-1 beta for 12 approximately 24 h caused inducible NO synthase mRNA expression in vascular smooth muscle cells, whereas aldosterone had a suppressive effect on its expression. Aldosterone also decreased interleukin-1 beta-induced NO synthase protein accumulation. These results indicate that aldosterone inhibits NO synthesis under interleukin-1 beta-stimulated conditions in vascular smooth muscle cells.
Ikeda
U
U
Department of Cardiology, Jichi Medical School, Tochigi, Japan.
Kanbe
T
T
Nakayama
I
I
Kawahara
Y
Y
Yokoyama
M
M
Shimada
K
K
D000450:Aldosterone / Q000494:pharmacology*; D000704:Analysis of Variance; D000818:Animals; D001013:Aorta, Thoracic; D015152:Blotting, Northern; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D006801:Humans; D015151:Immunoblotting; D007375:Interleukin-1 / Q000494:pharmacology; D009131:Muscle, Smooth, Vascular / Q000187:drug effects* / Q000378:metabolism; D009569:Nitric Oxide / Q000096:biosynthesis*; D019001:Nitric Oxide Synthase / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
D007375:Interleukin-1; D012333:RNA, Messenger; D009569:Nitric Oxide; D000450:Aldosterone; D019001:Nitric Oxide Synthase