Induction of tyrosine phosphorylation and translocation of ezrin by hepatocyte growth factor/scatter factor.
Ezrin is a member of the TERM family and is a key protein in cytoplasmic membrane-cytoskeleton interactions. This study showed that hepatocyte growth factor/scatter factor (HGF/SF), a cytokine known to regulate motility, morphogenesis and growth of cells, stimulated the tyrosine phosphorylation of ezrin in a human colon epithelial cell line, HT115. After HGF/SF stimulation, ezrin translocated from the cytosol and generalised membrane to the areas of ruffled membrane as visualised by indirect immunofluorescent and immunogold electron microscopy. This effect was inhibited by genistein, a tyrosine kinase inhibitor. It is concluded that HGF/SF induces ezrin translocation by stimulation of its tyrosine phosphorylation and that this plays a key role in HGF/SF induced membrane ruffling.
Jiang
W G
WG
University Department of Surgery, University of Wales College of Medicine, Cardiff, UK.
Hiscox
S
S
Singhrao
S K
SK
Puntis
M C
MC
Nakamura
T
T
Mansel
R E
RE
Hallett
M B
MB
D000199:Actins / Q000378:metabolism; D002451:Cell Compartmentation; D002462:Cell Membrane / Q000648:ultrastructure; D003598:Cytoskeletal Proteins; D004791:Enzyme Inhibitors / Q000494:pharmacology; D019833:Genistein; D017228:Hepatocyte Growth Factor / Q000502:physiology*; D006801:Humans; D007150:Immunohistochemistry; D007529:Isoflavones / Q000494:pharmacology; D010750:Phosphoproteins / Q000378:metabolism*; D010766:Phosphorylation; D019000:Phosphotyrosine / Q000378:metabolism*; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors; D014407:Tumor Cells, Cultured; D000099149:Ezrin
D000199:Actins; D003598:Cytoskeletal Proteins; D004791:Enzyme Inhibitors; D019833:Genistein; D017228:Hepatocyte Growth Factor; D007529:Isoflavones; D010750:Phosphoproteins; D019000:Phosphotyrosine; D011505:Protein-Tyrosine Kinases; D000099149:Ezrin