title: Cold storage effects on egg hatch in laboratory-reared Culicoides variipennis sonorensis (Diptera: Ceratopogonidae).
abstract: The effects of cold storage (5 degrees C) on the hatching rates of laboratory-reared Culicoides variipennis sonorensis eggs were examined. Mortality increased with storage time. Average maximum embryo survivorship for 4 trials was 55.0 +/- 4.2 (+/- SEM) days. Alternating daily cycles of high and then low mean hatching rates occurred and possibly were due to location differences in temperature within the temperature-controlled rearing system. During cold storage at 5 degrees C, C. v. sonorensis eggs may be kept for ca. 28 days with an anticipated hatching rate of about 50%.
authors.affiliation: U.S. Department of Agriculture, Arthropod-borne Animal Diseases Research Laboratory, Laramie, WY 82071-3965, USA.
authors.forename: G J
authors.initials: GJ
authors.lastname: Hunt
authors.forename: W J
authors.initials: WJ
authors.lastname: Tabachnick
mesh_terms: D000818:Animals; D002519:Ceratopogonidae; D003080:Cold Temperature; D015925:Cryopreservation; D005260:Female; D008027:Light; D010063:Ovum