[The influence of load size on the result of ethylene oxide gas sterilization].
The purpose of the study was establishing whether the size of the load--that is the degree of filling of sterilizer chamber with medical instruments--could have an effect on the time needed for eradication of all microorganisms subjected to the action of ethylene oxide. The test organism used was the strain of Bacillus subtilis var. niger ATTC 9372. Tests were prepared with about 3.6 x 10(6) spores per one test. The tested samples were exposed to ethylene oxide at concentration of 750 mg/l, at 50-80 degrees C, at 40% humidity during 30, 60, 120, 180, 240 and 300 minutes in three variants: in empty sterilizer chamber im chamber loaded with plastic objects (polyethylene and polypropylene)--600 g weight in chamber with similar load weighing 1200 g. The obtained results showed that the degree of filling of sterilizer chamber influenced the efficiency of gas sterilization with ethylene oxide. The effectiveness of each sterilization process with ethylene oxide should be controlled using biological indicators.
Jakimiak
B
B
Zakład Zwalczania Skazeń Biologicznych Państwowego Zakładu Higieny w Warszawie.
D001412:Bacillus subtilis / Q000302:isolation & purification; D005027:Ethylene Oxide; D007202:Indicators and Reagents; D010969:Plastics; D013170:Spores / Q000302:isolation & purification; D013242:Sterilization / Q000379:methods*; D013525:Surgical Instruments
D007202:Indicators and Reagents; D010969:Plastics; D005027:Ethylene Oxide
Wpływ wielkości załadunku na efektywność sterylizacji gazowej tlenkiem etylenu.