Nucleotide sequence of salicylate hydroxylase gene and its 5'-flanking region of Pseudomonas putida KF715.
The salicylate hydroxylase, a flavoprotein monooxygenase, catalyzes the decarboxylative hydroxylation of salicylate to form catechol. Nucleotide sequence of a salicylate hydroxylase gene and its 5'-flanking region in chromosomal DNA of Pseudomonas putida KF715 was analyzed. The salicylate hydroxylase was encoded in an open reading frame with 1308 base pairs which can encode a polypeptide of molecular weight 48 kDa with 435 amino acids. The open reading frame was preceded by a putative ribosome-binding sequence. A predicted amino acid sequence of the salicylate hydroxylase exhibited 84% identity with corresponding enzyme encoded in NAH7 plasmid, and 20 to 30% homologies with other similar flavoprotein monooxygenases. A 5'-flanking sequence of the salicylate hydroxylase gene exhibited extensive homology with promoter and nahR-binding site of sal operon in NAH7 plasmid.
Lee
J
J
College of Pharmacy, Chungbuk National University, Cheongju, Korea.
Oh
J
J
Min
K R
KR
Kim
Y
Y
D000595:Amino Acid Sequence; D001483:Base Sequence; D015964:Gene Expression Regulation, Bacterial; D006899:Mixed Function Oxygenases / Q000235:genetics*; D008969:Molecular Sequence Data; D009876:Operon; D010957:Plasmids; D016958:Pseudomonas putida / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D012045:Regulatory Sequences, Nucleic Acid; D015183:Restriction Mapping; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid
D012333:RNA, Messenger; D006899:Mixed Function Oxygenases; C022619:salicylate 1-monooxygenase