T-vector cloning and high performance PCR with SuperTth from Thermus thermophilus.
The SuperTth DNA polymerase from Thermus thermophilus exhibits template-independent terminal transferase (extendase) activity. This enzyme is proposed as a cheap alternative for both high performance PCR as well as quick T-vector cloning of amplicons, including reverse transcription and cDNA cloning.
Leal
J F
JF
Departamento de Bioquímica y Biología Molecular, Facultad de Veterinaria, Córdoba, Spain.
López-Barea
J
J
Dorado
G
G
D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods*; D004274:DNA, Recombinant; D004259:DNA-Directed DNA Polymerase / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D016963:Thermus thermophilus / Q000201:enzymology*
D004274:DNA, Recombinant; C093010:DNA polymerase, Thermus thermophilus; D004259:DNA-Directed DNA Polymerase