[Selective inhibition of replication of human cytomegalovirus by desferrioxamine in vitro and in vivo (case report)].
In AIDS patients cytomegalovirus (HCMV) retinitis is one of the most frequent opportunistic infections. Antiviral therapy aims at preserving vision as long as possible, but more and more HCMV isolates are proving to be resistant to ganciclovir (GCV) and foscarnet (PFA) in vitro. We tested whether desferrioxamine (DFO), an iron chelator with antiherpetic activity, can inhibit clinical virus isolates and laboratory strains. Clinical isolates were obtained from urine samples of AIDS patients with HCMV retinitis. The concentrations of DFO required for 50% and 90% reduction of the production of HCMV in several HCMV strains ranged from 3.1 to 4.9 microns and from 14.2 to 17.3 microns, respectively, Inhibitory effects of DFO on HCMV replication were completely prevented by co-incubation with stoichiometric amounts of FE3+.


METHODS
DFO was administered by daily dose of 1 g i.v. to a patient with AIDS in whom HCMV retinitis continued to progress despite combination therapy with GCV and PFA. The addition of DFO to the combination inhibited progression of the disease. No relapse of HCMV retinitis was seen with 3 months of DFO therapy. The treatment was free of side effects.
Gümbel
H
H
Zentrum der Augenheilkunde, Universität Frankfurt/Main.
Cinatl
J
J
Rabenau
H
H
Vogel
J U
JU
Doerr
H W
HW
Ohrloff
C
C
D017088:AIDS-Related Opportunistic Infections / Q000188:drug therapy* / Q000821:virology; D000328:Adult; D000998:Antiviral Agents / Q000008:administration & dosage; D003587:Cytomegalovirus / Q000187:drug effects*; D017726:Cytomegalovirus Retinitis / Q000188:drug therapy* / Q000821:virology; D003676:Deferoxamine / Q000008:administration & dosage*; D004305:Dose-Response Relationship, Drug; D015774:Ganciclovir / Q000008:administration & dosage; D006801:Humans; D007262:Infusions, Intravenous; D008297:Male; D017262:Siderophores / Q000008:administration & dosage*; D014776:Virus Cultivation; D014779:Virus Replication / Q000187:drug effects*
D000998:Antiviral Agents; D017262:Siderophores; D003676:Deferoxamine; D015774:Ganciclovir
Selektive Hemmung der Replikation des humanen Zytomegalievirus durch Desferrioxamin in vitro und in vivo (Fallbericht).