Intercellular adhesion molecule-3 on endothelial cells. Expression in tumors but not in inflammatory responses.
Intercellular adhesion molecule-3 (ICAM-3) was identified as the third counter-receptor for lymphocyte function-associated antigen-1. ICAM-3 is absent on endothelial cells in normal tissues but found on endothelial cells in lymphomas. Here, we examined ICAM-3 expression on vascular endothelial cells in lymphomas, nonlymphoid malignancies, benign tumors, and inflammatory diseases. We compared the expression of ICAM-3 on endothelial cells with the severity of inflammatory infiltrates and with the presence of E-selectin and VCAM-1. We found that ICAM-3 expression on endothelial cells was high on both benign and malignant tumors whereas it was low in inflammatory diseases. In contrast to E-selectin, ICAM-3 expression on endothelial cells was not correlated to the severity of inflammatory infiltrates. In hemangiomas, we showed by Northern blot analysis and immunocytochemistry that ICAM-3 expression was induced and that it was localized in immature areas that sustain the early stages of angiogenesis. Therefore, expression of ICAM-3 on blood vessels does not seem to play a role in the recruitment of leukocytes during inflammation but rather is correlated with angiogenesis and tumor development.
Patey
N
N
Service d'Anatomie et de Cytologie Pathologiques, Hôpital Necker-Enfants Malades, Paris, France.
Vazeux
R
R
Canioni
D
D
Potter
T
T
Gallatin
W M
WM
Brousse
N
N
D015703:Antigens, CD; D000943:Antigens, Differentiation; D014408:Biomarkers, Tumor; D015152:Blotting, Northern; D015815:Cell Adhesion Molecules / Q000032:analysis / Q000096:biosynthesis*; D019040:E-Selectin / Q000032:analysis / Q000096:biosynthesis; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D006391:Hemangioma / Q000737:chemistry / Q000378:metabolism; D006689:Hodgkin Disease / Q000378:metabolism; D006801:Humans; D007124:Immunoenzyme Techniques; D007249:Inflammation / Q000378:metabolism*; D008221:Lymphoid Tissue / Q000378:metabolism; D008223:Lymphoma / Q000737:chemistry / Q000378:metabolism*; D008228:Lymphoma, Non-Hodgkin / Q000378:metabolism; D009369:Neoplasms / Q000378:metabolism*; D009389:Neovascularization, Pathologic / Q000503:physiopathology; D019010:Vascular Cell Adhesion Molecule-1 / Q000032:analysis / Q000096:biosynthesis
D015703:Antigens, CD; D000943:Antigens, Differentiation; D014408:Biomarkers, Tumor; D015815:Cell Adhesion Molecules; D019040:E-Selectin; C483616:ICAM3 protein, human; D019010:Vascular Cell Adhesion Molecule-1