A new type soluble guanylyl cyclase, which contains a kinase-like domain: its structure and expression.
A new type soluble guanylyl cyclase, designated as ksGC (kinase-like domain containing soluble guanylyl cyclase), was identified from a kidney cDNA library. By Northern blot analysis, expression of this novel guanylyl cyclase mRNA was found in lung, kidney and skeletal muscle. Although ksGC is a soluble type guanylyl cyclase, it is not a member of soluble alpha or beta guanylyl cyclases. The ksGC contains in its N-terminal region a protein kinase-like domain, which is usually a characteristic feature only of particulate type guanylyl cyclases. This unique feature of ksGC suggests a novel pathway of cGMP synthesis.
Kojima
M
M
Department of Biochemistry, National Cardiovascular Center Research Institute, Osaka, Japan.
Hisaki
K
K
Matsuo
H
H
Kangawa
K
K
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers / Q000737:chemistry; D015870:Gene Expression; D006162:Guanylate Cyclase / Q000737:chemistry* / Q000235:genetics; D007668:Kidney / Q000201:enzymology; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D012995:Solubility
D017931:DNA Primers; D012333:RNA, Messenger; D006162:Guanylate Cyclase