Molecular cloning, sequencing, and expression in Escherichia coli of the chalcone synthase gene.
Flavonoids form a class of secondary metabolites that are abundant in all higher plants. They serve important functions in flower pigmentation. Chalcone Synthase (CHS) is a key enzyme in the biosynthesis of all classes of flavonoids; therefore, variation of the expression of CHS might change the color of flowers. We cloned CHS-A gene from Petunia hybrida, which has 99% sequence homology with those that have been cloned abroad. The sequence of the coding region is 1170bp and encodes a protein of 39kDa. CHS-A gene is also expressed high-efficiently in E. coli. All this work establishes a sound basis for future research on the impact of the CHS-A gene on flower pigmentation.
Shao
L
L
National Laboratory of Protein Engineering and Plant Genetic Engineering, Peking University, Beijing, China.
Li
Y
Y
Pan
A
A
Cheng
Z
Z
Chen
M
M
D000217:Acyltransferases / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D015870:Gene Expression / Q000235:genetics; D017343:Genes, Plant / Q000235:genetics; D008969:Molecular Sequence Data; D010944:Plants / Q000201:enzymology / Q000235:genetics; D012313:RNA / Q000032:analysis; D011994:Recombinant Proteins / Q000235:genetics; D017422:Sequence Analysis, DNA
D018076:DNA, Complementary; D011994:Recombinant Proteins; D012313:RNA; D000217:Acyltransferases; C020141:flavanone synthetase