[Establishment of a cisplatin-resistant new human endometrial adenocarcinoma cell line, Sawano cells].
A new human endometrial adenocarcinoma cell line, Sawano cells, was established from an endometrial adenocarcinoma from a 61-year-old woman and has been maintained in vitro for more than 2 years and 10 months. The cells were found to form a monolayer in a mosaic fashion and to tend to pile up. Population doubling time was 43.2 hours at the 10th generation. The modal chromosomal number of the cell fell in a diploid range. Histology of the tumor induced in athymic mice showed it to be a moderately differentiated adenocarcinoma which closely resembled the original human tumor. Estrogen and progesterone receptors were not demonstrated in the in vitro culture cells and in the tumors induced in nude mice, though they were positively demonstrated in the original tumor. The cells had intrinsic cisplatin-resistance (50% inhibition concentration:6.63 micrograms/ml) at 120 hours of contact. We believe this cell line with help us to investigate the biological mechanisms of CDDP resistance.
Satoh
T
T
Department of Obstetrics and Gynecology, University of Tsukuba, Japan.
Nishida
M
M
Miyazaki
Y
Y
Sugita
M
M
Arai
Y
Y
Oki
A
A
Kono
K
K
Tsunoda
H
H
Kasahara
K
K
Kubo
T
T
D000230:Adenocarcinoma / Q000235:genetics / Q000473:pathology*; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002945:Cisplatin / Q000494:pharmacology*; D004171:Diploidy; D019008:Drug Resistance, Neoplasm; D016889:Endometrial Neoplasms / Q000235:genetics / Q000473:pathology*; D005260:Female; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D008875:Middle Aged; D009368:Neoplasm Transplantation; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; D002945:Cisplatin