title: Response of a yeast glycogen synthase gene to stress.
abstract: In the yeast Saccharomyces cerevisiae, glycogen synthase is encoded by two genes: GSY1 and GSY2. The activity of the enzymes increases as cultures enter the stationary phase of growth. Using a GSY2::lacZ fusion gene, we have demonstrated that the increase in glycogen synthase activity resulted, at least in part, from an increase in the level of the protein rather than simply from a change in its phosphorylation state. Northern blot analysis showed a parallel increase in the level of the GSY2 mRNA, which is consistent with transcriptional activation of GSY2. Deletion analysis identified three regions upstream of GSY2 which are involved in GSY2 expression: regions A (-390 to -347 relative to the start of translation), B (-252 to -209) and C (-209 to -167). Region A or C independently activated expression of GSY2. In contrast, region B alone yielded only modest expression. Expression of GSY2 is induced by growth to stationary phase, heat shock or nitrogen starvation. Response to these stressors is mediated by elements within regions A and C. These elements appear to be related to the stress-response elements found in other stress-responsive genes.
authors.affiliation: Department of Biochemistry, University of Minnesota, Minneapolis 55455, USA.
authors.forename: H T
authors.initials: HT
authors.lastname: Ni
authors.forename: D C
authors.initials: DC
authors.lastname: LaPorte
mesh_terms: D001483:Base Sequence; D015152:Blotting, Northern; D002455:Cell Division; D003470:Culture Media; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D006003:Glycogen / Q000096:biosynthesis; D006006:Glycogen Synthase / Q000235:genetics* / Q000378:metabolism*; D006358:Hot Temperature; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics* / Q000502:physiology; D017384:Sequence Deletion; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D003470:Culture Media; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D006003:Glycogen; D006006:Glycogen Synthase; D001616:beta-Galactosidase