Molecular detection of primary bladder cancer by microsatellite analysis.
Microsatellite DNA markers have been widely used as a tool for the detection of loss of heterozygosity and genomic instability in primary tumors. In a blinded study, urine samples from 25 patients with suspicious bladder lesions that had been identified cystoscopically were analyzed by this molecular method and by conventional cytology. Microsatellite changes matching those in the tumor were detected in the urine sediment of 19 of the 20 patients (95 percent) who were diagnosed with bladder cancer, whereas urine cytology detected cancer cells in 9 of 18 (50 percent) of the samples. These results suggest that microsatellite analysis, which in principle can be performed at about one-third the cost of cytology, may be a useful addition to current screening methods for detecting bladder cancer.
Mao
L
L
Department of Otolaryngology--Head and Neck Surgery, Johns Hopkins University School of Medicine, Baltimore, MD 21205-2196, USA.
Schoenberg
M P
MP
Scicchitano
M
M
Erozan
Y S
YS
Merlo
A
A
Schwab
D
D
Sidransky
D
D
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002872:Chromosome Deletion; D002899:Chromosomes, Human, Pair 9; D004273:DNA, Neoplasm / Q000235:genetics / Q000652:urine*; D005260:Female; D005819:Genetic Markers; D006579:Heterozygote; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats; D008875:Middle Aged; D009367:Neoplasm Staging; D010865:Pilot Projects; D016133:Polymerase Chain Reaction; D001749:Urinary Bladder Neoplasms / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology / Q000652:urine; D014556:Urine / Q000166:cytology
D004273:DNA, Neoplasm; D005819:Genetic Markers