title: Nucleotide sequence of the gene encoding beta-isopropylmalate dehydrogenase (leuB) from Bacteroides fragilis.
abstract: The complete nucleotide sequence of the gene (leuB) coding for beta-isopropylmalate dehydrogenase of Bacteroides fragilis was determined. An open reading frame of 1,061 nucleotides was detected that could encode a polypeptide of 353 amino acid residues with a calculated molecular mass of 39,179 Da. The deduced amino acid sequence of the beta-isopropylmalate dehydrogenase from B. fragilis showed substantial sequence similarity with the beta-isopropylmalate dehydrogenases from other bacteria.
authors.affiliation: Department of Bacteriology, School of Medicine, University of Tokushima, Japan.
authors.forename: M R
authors.initials: MR
authors.lastname: Sarker
authors.forename: S
authors.initials: S
authors.lastname: Akimoto
authors.forename: H
authors.initials: H
authors.lastname: Ugai
authors.forename: T
authors.initials: T
authors.lastname: Kuwahara
authors.forename: Y
authors.initials: Y
authors.lastname: Ohnishi
mesh_terms: D050539:3-Isopropylmalate Dehydrogenase; D000429:Alcohol Oxidoreductases / Q000235:genetics*; D000595:Amino Acid Sequence; D001441:Bacteroides fragilis / Q000201:enzymology* / Q000235:genetics; D001483:Base Sequence; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016366:Open Reading Frames / Q000235:genetics; D017386:Sequence Homology, Amino Acid
chemical_list: D000429:Alcohol Oxidoreductases; D050539:3-Isopropylmalate Dehydrogenase