Effect of carbohydrate source on lipid metabolism in lactating mice and on pup development.
The effect of long-term sucrose intake on lipid metabolism and milk composition was examined in lactating mice and correlated with the development of their pups. Female mice were weaned onto semi-purified diets containing 685 g starch or sucrose/kg diet. Food intake was similar on the two diets. At mid-lactation, milk was collected and analysed. Milk from sucrose-fed dams had a lower percentage of linoleic acid (18:2n-6) and a higher percentage of oleic acid (18:1n-9), although milk fat content was similar. Hepatic fatty acid synthesis (as measured by 3H2O incorporation into fatty acid) was three times faster in sucrose-fed than in starch-fed dams. Dietary carbohydrate had no effect on fatty acid synthesis or functional lipoprotein lipase (EC 3.1.1.34) activity in mammary gland, nor on plasma triacylglycerol concentration. Pups from first litters were killed at 11-12 d and body composition analysed. Pups born to sucrose-fed dams were significantly heavier with increased body fat and higher levels of plasma glucose and triacylglycerol. Litters of sucrose- and starch-fed dams were cross-fostered at birth and killed at 10-12 d. Analysis of variance indicated that maternal sucrose intake, both during pregnancy and lactation, increased body fat of offspring. The increases in body weight and plasma triacylglycerol of preweaning offspring were caused primarily by maternal sucrose intake during lactation.
Ghusain-Choueiri
A A
AA
School of Biological Sciences, Queen Mary and Westfield College, London.
Rath
E A
EA
D000818:Animals; D000831:Animals, Newborn / Q000254:growth & development*; D001786:Blood Glucose / Q000378:metabolism; D001823:Body Composition; D001835:Body Weight; D004040:Dietary Carbohydrates / Q000008:administration & dosage / Q000494:pharmacology*; D005227:Fatty Acids / Q000378:metabolism; D005260:Female; D007774:Lactation / Q000378:metabolism*; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000032:analysis; D050356:Lipid Metabolism; D008099:Liver / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008892:Milk / Q000737:chemistry / Q000378:metabolism; D019301:Oleic Acid; D009829:Oleic Acids / Q000032:analysis; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D013213:Starch / Q000008:administration & dosage; D013395:Sucrose / Q000008:administration & dosage / Q000494:pharmacology*; D014280:Triglycerides / Q000097:blood
D001786:Blood Glucose; D004040:Dietary Carbohydrates; D005227:Fatty Acids; D008041:Linoleic Acids; D009829:Oleic Acids; D014280:Triglycerides; D019301:Oleic Acid; D013395:Sucrose; D013213:Starch; D019787:Linoleic Acid