title: Postnatal development of the complexes of the electron transport chain in synaptic mitochondria from rat brain.
abstract: The postnatal development of the complexes of the electron transport chain in mitochondria isolated from rat brain synaptosomes was investigated. Synaptosomal brain mitochondria were isolated from rats aged 10-60 days, and the activities of mitochondrial complex I, complex II-III, complex IV and complex V were measured. There was a significant increase in the activity of II-III from day 10 to day 15 and complex IV from day 10 to day 21, thereafter the activities of complexes I-III and IV did not change significantly. The activity of complex I did not change significantly during the period 10-60 days post partum. In synaptic mitochondria, complex V activity was higher than in non-synaptic mitochondria, whereas the activity of complex I was lower than in non-synaptic mitochondria. These data show that the complexes of the respiratory chain within synaptic mitochondria have activities different from those of non-synaptic mitochondria and may have major implications for the relative susceptibility of mitochondria in different brain cell types to neurotoxins such as MPP+, hypoxic/ischaemic damage and oxidative stress.
authors.affiliation: Department of Neurochemistry, University of London, UK.
authors.forename: A
authors.initials: A
authors.lastname: Almeida
authors.forename: K J
authors.initials: KJ
authors.lastname: Brooks
authors.forename: I
authors.initials: I
authors.lastname: Sammut
authors.forename: J
authors.initials: J
authors.lastname: Keelan
authors.forename: G P
authors.initials: GP
authors.lastname: Davey
authors.forename: J B
authors.initials: JB
authors.lastname: Clark
authors.forename: T E
authors.initials: TE
authors.lastname: Bates
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000254:growth & development* / Q000378:metabolism / Q000648:ultrastructure; D002950:Citrate (si)-Synthase / Q000378:metabolism; D004579:Electron Transport / Q000502:physiology; D042963:Electron Transport Complex II; D014450:Electron Transport Complex III / Q000378:metabolism; D003576:Electron Transport Complex IV / Q000378:metabolism; D005260:Female; D066298:In Vitro Techniques; D008297:Male; D008928:Mitochondria / Q000201:enzymology / Q000378:metabolism*; D025261:Mitochondrial Proton-Translocating ATPases; D009097:Multienzyme Complexes / Q000378:metabolism; D009243:NAD / Q000378:metabolism; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000378:metabolism; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000378:metabolism; D006180:Proton-Translocating ATPases / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013385:Succinate Dehydrogenase / Q000378:metabolism; D013569:Synapses / Q000201:enzymology / Q000378:metabolism*; D013574:Synaptosomes / Q000201:enzymology / Q000378:metabolism*
chemical_list: D009097:Multienzyme Complexes; D009419:Nerve Tissue Proteins; C021983:complex V (mitochondrial oxidative phosphorylation system); D009243:NAD; D010088:Oxidoreductases; D042963:Electron Transport Complex II; D013385:Succinate Dehydrogenase; D016660:NAD(P)H Dehydrogenase (Quinone); D003576:Electron Transport Complex IV; D002950:Citrate (si)-Synthase; D000110:Acetylcholinesterase; D025261:Mitochondrial Proton-Translocating ATPases; D006180:Proton-Translocating ATPases; D014450:Electron Transport Complex III