Human lymphocyte cDNA ordered library analyzed by 2D gel electrophoresis. 3. Analysis of individual clones.
We pursued the goal of obtaining global information on cDNA libraries from lymphocytes by combining data on the genes with data on the expressed proteins. Ordered libraries enabled us to establish the properties of the clones by direct retrieval of individual clones from the matrix (in our system a 24 x 16 x 12 array). We have picked-upon inspection of radiofluorographs obtained from the pools of the library--39 clones and performed a 2D gel analysis on the cell free expressed clonal products and have established also their partial nucleotide sequences. Details on two such clones are reported; clone CEM1001 is a new gene, while clone CEM1002 is a gene for human elongation factor 1-beta.
Béhar
G
G
Basel Institute for Immunology, Switzerland.
Coleclough
C
C
Houlgatte
R
R
Auffray
C
C
Lefkovits
I
I
D001483:Base Sequence; D002999:Clone Cells; D018076:DNA, Complementary / Q000032:analysis*; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D015723:Gene Library; D006801:Humans; D008214:Lymphocytes / Q000166:cytology*; D008969:Molecular Sequence Data; D010865:Pilot Projects
D018076:DNA, Complementary