title: Central glucopenia induced by 2-deoxy-D-glucose stimulates somatostatin secretion in the rat.
abstract: The mechanisms involved in 2-deoxy-D-glucose (2-DG)-induced growth hormone (GH) suppression in the rat were examined. Conscious male rats were given 2-DG by intracerebroventricular (icv) injection and the pulsatile GH secretion was monitored for 6 h. The single icv injection of 2-DG (8 mg/rat) eliminated pulsatile GH secretion in conscious rats. Pretreatment with somatostatin (SS) antiserum completely restored the suppressed GH secretion in the 2-DG treated rats. Hypothalamic GH-releasing hormone (GRH) and SS mRNA levels were not altered by single and multiple icv injections of 2-DG. These findings suggest that 2-DG-induced GH suppression is primarily due to hypersecretion of SS without a significant change at the transcription level in the rat.
authors.affiliation: First Department of Internal Medicine, Kagawa Medical School, Miki-Cho, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Sato
authors.forename: K
authors.initials: K
authors.lastname: Murao
authors.forename: S
authors.initials: S
authors.lastname: Matsubara
authors.forename: M
authors.initials: M
authors.lastname: Niimi
authors.forename: K
authors.initials: K
authors.lastname: Chihara
authors.forename: J
authors.initials: J
authors.lastname: Takahara
mesh_terms: D000818:Animals; D003847:Deoxyglucose / Q000008:administration & dosage / Q000494:pharmacology*; D005947:Glucose / Q000172:deficiency*; D013006:Growth Hormone / Q000378:metabolism; D013007:Growth Hormone-Releasing Hormone / Q000235:genetics; D007031:Hypothalamus / Q000378:metabolism; D007116:Immunization, Passive; D007276:Injections, Intraventricular; D008297:Male; D010507:Periodicity; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013004:Somatostatin / Q000235:genetics / Q000276:immunology / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D013004:Somatostatin; D013006:Growth Hormone; D013007:Growth Hormone-Releasing Hormone; D003847:Deoxyglucose; D005947:Glucose