Determination of ibuprofen in erythrocytes and plasma by high performance liquid chromatography.
A high-performance liquid chromatographic (HPLC) method is described for the determination of ibuprofen in isolated erythrocytes and plasma. Before HPLC analysis ibuprofen was isolated by liquid-liquid extraction from these biological matrices; methylene chloride proved to be the best of the organic solvents tested. For the sample of erythrocytes it was necessary to carry out haemolysis prior to their extraction. HPLC was performed on a C-18 column with a mobile phase of methanol-water (220:100, v/v) acidified with perchloric acid to pH 3. Ultraviolet detection was at 222 nm. This method has been applied to the quantification of ibuprofen in rabbit erythrocytes and plasma for a pharmacokinetics study.
Sochor
J
J
Department of Pharmaceutical Chemistry and Drug Control, Faculty of Pharmacy, Charles University, Králové, Czech Republic.
Klimes
J
J
Sedlácek
J
J
Zahradnícek
M
M
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000097:blood* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid; D004912:Erythrocytes / Q000737:chemistry*; D007052:Ibuprofen / Q000097:blood* / Q000493:pharmacokinetics; D016014:Linear Models; D011817:Rabbits; D012680:Sensitivity and Specificity
D000894:Anti-Inflammatory Agents, Non-Steroidal; D007052:Ibuprofen