[Analysis of heavy chain immunoglobulins gene and T-cell receptor delta gene in children with acute leukemia].
IgH gene, TCR delta gene rearrangements and Tal-1 gene deletion were analysed by using PCR, Southern blot and DNA sequencing methods in newly diagnosed BM samples from children with AL. The DNAs from leukemic cells in 102 children were detected with PCR. The results showed that IgH gene rearrangement mainly occurred in B Precursor ALL (37/49). Six PCR products were further analysed by DNA sequencing. Less homogene and bias of JH gene usage were found in analysed DNA sequences. V delta 2-D delta 3 rearrangement of 78 samples from ALL was analysed with PCR method. V delta 2-D delta 3 rearrangements mainly observed in B precursor ALL and related HAL. V delta 2-D delta 2-N-D delta 3 rearrangement was found in a sequence of PCR product. Three cases of Tal-1 gene deletion were observed in all studied 70 samples of AL. They all were in stage I of thymus differentiation. We conclud that PCR detection of those genes are useful in the diagnosis of clonality of AL, DNA sequencing of PCR products is the base of preparing clonal specific probes, and dynamic analysis of IgH gene rearrangement using PCR may be helpful in detection of residual clones.
Pan
Q
Q
Affiliated Children's Hospital, Zhejiang Medical University, Hangzhou.
Chou
Y
Y
Hong
W
W
D002648:Child; D017353:Gene Deletion; D015321:Gene Rearrangement; D016694:Gene Rearrangement, delta-Chain T-Cell Antigen Receptor; D005803:Genes, Immunoglobulin; D006801:Humans; D016133:Polymerase Chain Reaction; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics*; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000235:genetics*; D017422:Sequence Analysis, DNA
D016692:Receptors, Antigen, T-Cell, gamma-delta