title: A comparative study of platelets stored in polyvinyl chloride containers plasticised with butyryl trihexyl citrate or triethylhexyl trimellitate.
abstract: Platelet concentrates (PCs), stored for 5 days in PL 2209, a new polyvinyl chloride (PVC) storage container plasticised with butyryl trihexyl citrate, were compared with those stored in PL 1240, a PVC platelet container plasticised with triethylhexyl trimellitate. In part 1 of the study, pooled platelet-rich plasma (PRP) was aliquoted into each type of pack and pH, pCO2, pO2, hypotonic shock response, aggregation responses, lactate, glucose and ATP concentrations, and lactate dehydrogenase and beta-thromboglobulin release were compared at days 1, 3 and 5. In part 2, 12 volunteers gave a unit of blood on two separate occasions and PCs produced by the PRP method were stored in PL 2209 or PL 1240 for 5 days before autologous reinfusion of a 111In-labelled sample. In vitro results demonstrated that PL 2209 was more gas permeable than PL 1240. In part 2 of the study, at day 5, pCO2 was 3.13 +/- 0.62 versus 5.14 +/- 0.69 (p < 0.001), whilst pO2 was not significantly different for PL 2209 versus PL 1240, respectively. pH was better maintained in PL 2209 than in PL 1240 (7.38 +/- 0.13 vs. 7.24 +/- 0.10, respectively, p < 0.01) after storage for 5 days. These results were confirmed by those from part 1. In vivo data were similar for PC stored in the two plastics with a multiple-hit recovery of 40.9 +/- 12.1% for PL 2209 and 37.4 +/- 11.3% for PL 1240, and a multiple-hit survival of 4.89 +/- 1.20 days and 5.28 +/- 2.06 days for PL 2209 and PL 1240, respectively. gamma-Camera imaging of volunteers showed similar biodistribution of radiolabelled platelets stored in each container. These results demonstrate that PL 2209 is a suitable container for storage of PCs for 5 days.
authors.affiliation: National Blood Service, West Midlands Centre, Birmingham, UK.
authors.forename: V S
authors.initials: VS
authors.lastname: Turner
authors.forename: S G
authors.initials: SG
authors.lastname: Mitchell
authors.forename: S K
authors.initials: SK
authors.lastname: Kang
authors.forename: R J
authors.initials: RJ
authors.lastname: Hawker
mesh_terms: D001565:Benzoates / Q000737:chemistry*; D001782:Blood Donors; D001792:Blood Platelets / Q000737:chemistry* / Q000000981:diagnostic imaging; D001793:Blood Preservation / Q000379:methods*; D002087:Butyrates / Q000737:chemistry*; D002470:Cell Survival / Q000187:drug effects; D006801:Humans; D066298:In Vitro Techniques; D010968:Plasticizers / Q000737:chemistry*; D010976:Platelet Count; D011143:Polyvinyl Chloride / Q000737:chemistry*; D011877:Radionuclide Imaging
chemical_list: D001565:Benzoates; D002087:Butyrates; D010968:Plasticizers; C036101:tri-(2-ethylhexyl)trimellitate; C066757:butyryl-n-trihexylcitrate; D011143:Polyvinyl Chloride