Homologous conditioned medium enhances expression of TRH in hypothalamic neurons in primary culture.
Primary cultures of hypothalamic cells maintained in the presence of serum were either kept with homologous conditioned medium (CM) (i.e. only half of the medium was removed at each medium change) or without (total medium change). In cultures with homologous CM, TRH levels were increased. The effects of CMs from various intervals of the primary culture were tested. The strongest increases of TRH levels were obtained with CM from cultures enriched with hypothalamic glia.
Charli
J L
JL
Departamento de Genetica y Fisiologia Molecular, Universidad Nacional Autonoma de Mexico, Cuernavaca, Mor, Mexico.
Cruz
C
C
Redondo
J L
JL
Guerra
C
C
Joseph-Bravo
P
P
D000595:Amino Acid Sequence; D000818:Animals; D002452:Cell Count / Q000187:drug effects; D002478:Cells, Cultured; D017077:Culture Media, Conditioned; D003561:Cytarabine / Q000494:pharmacology; D007031:Hypothalamus / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D009414:Nerve Growth Factors / Q000096:biosynthesis*; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D013973:Thyrotropin-Releasing Hormone / Q000096:biosynthesis*
D017077:Culture Media, Conditioned; D009414:Nerve Growth Factors; D003561:Cytarabine; D013973:Thyrotropin-Releasing Hormone