Expression and regulation by insulin of GLUT 3 in UMR 106-01, a clonal rat osteosarcoma cell line.
Expression of the glucose transporter GLUT 3 is mainly restricted to neuronal tissues, with lower levels in testis and placenta. In addition, GLUT 3 has recently been reported in neonatal rat calvaria by in situ hybridisation. We report the co-expression of GLUT 1 and GLUT 3 mRNA and protein in UMR 106-01, a clonal osteosarcoma cell line. By semi-quantitative analysis we show that GLUT 3 protein is expressed at levels comparable to GLUT 1. Insulin stimulates glucose uptake in UMR 106-01 cells. GLUT 3 mRNA and protein are increased by chronic (16 h) treatment with insulin, and the increase in GLUT 3 mRNA is not inhibited by cycloheximide. Regulation of GLUT 3 mRNA by insulin has not been previously shown. UMR 106-01 represents a useful model for investigating regulation of GLUT 3 expression.
Thomas
D M
DM
University of Melbourne Department of Medicine, St Vincents Hospital, Fitzroy, Victoria, Australia.
Maher
F
F
Rogers
S D
SD
Best
J D
JD
D000818:Animals; D002999:Clone Cells; D015870:Gene Expression; D051272:Glucose Transporter Type 1; D051274:Glucose Transporter Type 3; D007328:Insulin / Q000494:pharmacology*; D009004:Monosaccharide Transport Proteins / Q000235:genetics / Q000378:metabolism*; D009419:Nerve Tissue Proteins; D012516:Osteosarcoma; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D014407:Tumor Cells, Cultured
D051272:Glucose Transporter Type 1; D051274:Glucose Transporter Type 3; D007328:Insulin; D009004:Monosaccharide Transport Proteins; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; C495679:Slc2a1 protein, rat; C495690:Slc2a3 protein, rat