title: Hodgkin's disease after transplantation.
abstract: Hodgkin's disease (HD) has seldom been reported after transplantation. Epstein-Barr virus (EBV) is present in about 50% of Reed-Sternberg cells in HD developing in immunocompetent individuals, but is more frequently found in HD of acquired immune deficiency syndrome patients. We report 7 cases of HD that occurred in transplant recipients. Clinical and pathological data and studies of EBV reveal specific features of HD after transplantation. Six patients received kidney transplants and 1 patient received combined kidney and pancreas transplantation. Immunosuppressive therapy consisted of cyclosporine, steroids, azathioprine, and antilymphocyte globulins. One patient received, in addition, anti-CD3 mAb therapy and an EBV+ B cell lymphoma developed. Retrospective EBV serological data from patients were collected. Tumors were classified according to pathology. EBV studies were conducted by immunohistochemical methods with monoclonal antibodies to EBV-latent membrane protein (LMP) or EBV-nuclear antigen 2 (EBNA2), and by in situ hybridization for latent nuclear EBV-early RNAs (EBERs). The mean lapse of time between transplantation and HD was 49 months. Six patients presented with enlarged lymph nodes and 1 patient presented with liver involvement. HD was classified as IA in 2 patients, IIA in 3 patients, IIIB in 1 patient, and IVB in 1 patient. Four patients had primary EBV infection after graft, before HD, and the others reactivated latent EBV infection. Histological subtypes were mixed cellularity in 6 cases and lymphocytic depletion in 1 case. Latent EBV infection was detected with EBERs in all tumors. Reed-Sternberg cells expressed LMP, and were negative for EBNA2 expression. Six patients were treated: 2 patients at stage I received radiotherapy, and relapsed within 1 year with a more advanced stage of HD; chemotherapy was indicated as primary therapy in 5 patients, and as salvage therapy in 2 patients; it was associated with radiotherapy in 4 patients. Immunosuppressive therapy was reduced in all patients. Four patients were alive and in complete remission 18, 25, 31, and 67 months after chemotherapy, with a functioning graft in 3 patients. Two patients died of infection. Mixed cellularity is the most frequent histological subtype observed in HD occurring in transplant patients. EBV is present in all Reed-Sternberg cells. Posttransplant HD shows similarities with human immunodeficiency virus-associated HD. These facts argue for a role of EBV infection and immunosuppression in the progression of HD after transplantation.
authors.affiliation: Inserm U80, Service d'Urologie, Département d' Hématologie, Hôpital Edouard Herriot, Lyon, France.
authors.forename: J L
authors.initials: JL
authors.lastname: Garnier
authors.forename: Y
authors.initials: Y
authors.lastname: Lebranchu
authors.forename: J
authors.initials: J
authors.lastname: Dantal
authors.forename: J
authors.initials: J
authors.lastname: Bedrossian
authors.forename: R
authors.initials: R
authors.lastname: Cahen
authors.forename: D
authors.initials: D
authors.lastname: Assouline
authors.forename: A
authors.initials: A
authors.lastname: Jaccard
authors.forename: F
authors.initials: F
authors.lastname: Fetissoff
authors.forename: A
authors.initials: A
authors.lastname: Moreau
authors.forename: X
authors.initials: X
authors.lastname: Martin
authors.forename: G
authors.initials: G
authors.lastname: Delsol
authors.forename: F
authors.initials: F
authors.lastname: Berger
authors.forename: J L
authors.initials: JL
authors.lastname: Touraine
mesh_terms: D000293:Adolescent; D000328:Adult; D006084:Graft Rejection / Q000517:prevention & control; D004854:Herpesvirus 4, Human / Q000302:isolation & purification; D006689:Hodgkin Disease / Q000209:etiology* / Q000503:physiopathology / Q000821:virology; D006801:Humans; D007166:Immunosuppressive Agents / Q000009:adverse effects; D016030:Kidney Transplantation / Q000009:adverse effects*; D016393:Lymphoma, B-Cell / Q000209:etiology / Q000503:physiopathology / Q000821:virology; D008297:Male; D008875:Middle Aged; D016035:Pancreas Transplantation / Q000009:adverse effects*
chemical_list: D007166:Immunosuppressive Agents