title: Molecular characterisation of UK isolates of barley yellow mosaic bymovirus.
abstract: Several isolates of barley yellow mosaic virus (BaYMV) from different sites in the UK, including some that were virulent on European resistant winter barley cultivars (resistance-breaking strain: BaYMV-2) and some that were not, were examined by RT-PCR, restriction mapping and sequencing of selected parts of the virus genome. Nucleotide and predicted amino acid sequences were determined for the 5'-terminal region, part of the NIa coding region and the coat protein coding region on RNA 1 and an area at the N-terminus of the 70-kDa protein coding region on RNA 2. The sequences differed from those previously reported for a BaYMV isolate from Japan and for two German isolates, one of which was of the BaYMV-2 strain. There were no strain-specific amino acid differences and the few, non-consecutive, nucleotide differences detected were probably not significant and were insufficient to develop a rapid diagnostic test to distinguish BaYMV-2 from other isolates. Restriction mapping of RNA 2 cDNA again showed no consistent strain-related differences. The differences previously reported between the two German isolates are probably not strain-related.
authors.affiliation: Department of Virology, Scottish Crop Research Institute, Invergowrie, Dundee, UK.
authors.forename: N N
authors.initials: NN
authors.lastname: Shi
authors.forename: M
authors.initials: M
authors.lastname: Zhu
authors.forename: J
authors.initials: J
authors.lastname: Chen
authors.forename: R
authors.initials: R
authors.lastname: Stratford
authors.forename: T M
authors.initials: TM
authors.lastname: Wilson
authors.forename: J F
authors.initials: JF
authors.lastname: Antoniw
authors.forename: I J
authors.initials: IJ
authors.lastname: Foulds
authors.forename: S A
authors.initials: SA
authors.lastname: MacFarlane
authors.forename: M J
authors.initials: MJ
authors.lastname: Adams
mesh_terms: D001483:Base Sequence; D002213:Capsid / Q000235:genetics; D017931:DNA Primers; D004279:DNA, Viral / Q000235:genetics; D001467:Hordeum / Q000821:virology*; D008969:Molecular Sequence Data; D017800:Potyvirus / Q000235:genetics* / Q000302:isolation & purification; D012367:RNA, Viral / Q000235:genetics; D015183:Restriction Mapping; D006113:United Kingdom
chemical_list: D017931:DNA Primers; D004279:DNA, Viral; D012367:RNA, Viral