title: Phosphorylation of caldesmon by mitogen-activated protein kinase with no effect on Ca2+ sensitivity in rabbit smooth muscle.
abstract: 1. Recombinant, activated mitogen-activated protein kinase (3.3 microM; p42mapk) phosphorylated caldesmon in phasic (rabbit portal vein) and tonic (rabbit femoral artery) smooth muscle strips permeabilized with Triton X-100. 2. Phosphorylation of caldesmon by p42mapk neither induced contraction of relaxed smooth muscle nor affected the Ca2+ sensitivity of submaximally contracted permeabilized phasic or tonic smooth muscle.
authors.affiliation: Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville 22908, USA.
authors.forename: G F
authors.initials: GF
authors.lastname: Nixon
authors.forename: K
authors.initials: K
authors.lastname: Iizuka
authors.forename: C M
authors.initials: CM
authors.lastname: Haystead
authors.forename: T A
authors.initials: TA
authors.lastname: Haystead
authors.forename: A P
authors.initials: AP
authors.lastname: Somlyo
authors.forename: A V
authors.initials: AV
authors.lastname: Somlyo
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000378:metabolism*; D002148:Calmodulin-Binding Proteins / Q000378:metabolism*; D004789:Enzyme Activation; D005263:Femoral Artery / Q000502:physiology; D066298:In Vitro Techniques; D008297:Male; D019950:Mitogen-Activated Protein Kinase 1; D009119:Muscle Contraction / Q000502:physiology*; D009131:Muscle, Smooth, Vascular / Q000378:metabolism*; D017830:Octoxynol; D010766:Phosphorylation; D011169:Portal Vein / Q000502:physiology; D011505:Protein-Tyrosine Kinases / Q000378:metabolism*; D011817:Rabbits
chemical_list: D002148:Calmodulin-Binding Proteins; D017830:Octoxynol; D011505:Protein-Tyrosine Kinases; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D019950:Mitogen-Activated Protein Kinase 1; D002118:Calcium