title: cDNA cloning and sequencing of 54kDa fragment of the 75kDa readthrough protein gene from beet necrotic yellow vein virus.
abstract: With viral RNA extracted from purified beet necrotic yellow vein virus (BNYVV) isolated from Inner Mongolia, the first strand of cDNA encoding a 54kDa fragment of the 75kDa readthrough protein on the RNA2 was synthesized by reverse transcription. A double-strand cDNA fragment of 1.5 kb was obtained after 30 cycles of PCR amplification. The fragment was ligated into and mapped on pGEM-7Zf(+). The result of sequence analysis shows that the 54kDa readthrough domain is 1509 nucleotides (nt). Compared with F13 isolate, the fragment shares 94.97% identity in terms of nucleotides with 3 nt deletion and 96.42% identity of deduced amino acids.
authors.affiliation: National Laboratory for Agrobiotechnology, Beijing Agricultural University, China.
authors.forename: J
authors.initials: J
authors.lastname: Yu
authors.forename: D
authors.initials: D
authors.lastname: Li
authors.forename: L
authors.initials: L
authors.lastname: Yang
authors.forename: Y
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D005814:Genes, Viral / Q000235:genetics; D008969:Molecular Sequence Data; D010942:Plant Viruses / Q000235:genetics*; D012328:RNA Viruses / Q000235:genetics*; D017422:Sequence Analysis, DNA; D014764:Viral Proteins / Q000235:genetics
chemical_list: D018076:DNA, Complementary; D014764:Viral Proteins