title: Induction of the adipose differentiation-related protein in liver of etomoxir-treated rats.
abstract: The effects of etomoxir, an irreversible carnitine palmitoyltransferase I inhibitor, on the liver protein pattern and on liver morphology were examined by two-dimensional gel electrophoresis in female Sprague-Dawley rats treated with 125 mg/kg/day etomoxir for 28 days. In livers of treated animals a protein spot was found which was not present in controls. The spot was identified by internal amino acid sequence analysis as the adipose differentiation-related protein (ADRP). The expression of ADRP in liver is a novel finding as the protein has been described previously as adipocyte-specific. Additionally we found histopathologic evidence of lipid accumulation in the livers of etomoxir rats. The data show that for each treated rat there was a good correlation between ADRP levels and degree of lipid droplet formation. This observation may suggest a potential relationship between drug-induced expression of ADRP in liver and lipid accumulation.
authors.affiliation: Sandoz Pharma Ltd, Basel, Switzerland. steiner@dstox.pharma.sandoz.ch.
authors.forename: S
authors.initials: S
authors.lastname: Steiner
authors.forename: D
authors.initials: D
authors.lastname: Wahl
authors.forename: B L
authors.initials: BL
authors.lastname: Mangold
authors.forename: R
authors.initials: R
authors.lastname: Robison
authors.forename: J
authors.initials: J
authors.lastname: Raymackers
authors.forename: L
authors.initials: L
authors.lastname: Meheus
authors.forename: N L
authors.initials: NL
authors.lastname: Anderson
authors.forename: A
authors.initials: A
authors.lastname: Cordier
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002334:Carnitine O-Palmitoyltransferase / Q000037:antagonists & inhibitors; D015180:Electrophoresis, Gel, Two-Dimensional; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D004852:Epoxy Compounds / Q000494:pharmacology*; D005260:Female; D008099:Liver / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D008565:Membrane Proteins / Q000378:metabolism*; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry; D000070780:Perilipin-2; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D004791:Enzyme Inhibitors; D004852:Epoxy Compounds; D008565:Membrane Proteins; D010455:Peptides; D000070780:Perilipin-2; C000602984:Plin2 protein, rat; D002334:Carnitine O-Palmitoyltransferase; C054207:etomoxir