[Immunohistological evaluation of esophageal cancer with antikeratin monoclonal antibodies].
In this study, I investigated the molecular weights of keratin-subunits in the tissue of esophageal cancer by enzyme-labeled antibody technique with four kinds of antikeratin monoclonal antibodies with different spectrums of reacting keratin-subunits. Further more, the correlation between the molecular weights of keratin-subunits and clinicopathological factors was evaluated, and that between the molecular weights and the prognosis was studied also. The positive rates of four kinds of primary antibodies did not correlate to clinical feature or prognosis, and each primary antibody showed different attitude regarding correlation to histopathological factors. I analyzed the molecular weights of keratin-subunits increased in the carcinoma tissue by the difference of staining intensities of four monoclonal antibodies. The histological findings of the cases with carcinoma tissues in which 48, 56 kd keratins increased were in high malignancy compared with other groups, and their prognosis was significantly poor. Examination of prognostic factors with multivariate analysis revealed that the increase of 48, 56 kd keratins in the carcinoma tissues was the most important determining factor of prognosis next to lymphatic invasion.
Tanimura
S
S
Second Department of Surgery, Osaka City University Medical School, Japan.
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor / Q000032:analysis*; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis* / Q000473:pathology; D004938:Esophageal Neoplasms / Q000175:diagnosis* / Q000473:pathology; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D007633:Keratins / Q000032:analysis* / Q000276:immunology; D008297:Male; D008875:Middle Aged; D008970:Molecular Weight; D015999:Multivariate Analysis; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D009367:Neoplasm Staging; D011379:Prognosis
D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor; D007633:Keratins