Specific expression of surface interferon-gamma on interferon-gamma producing T cells from mouse and man.
Interferon (IFN)-gamma is a potent immunoregulatory protein secreted by CD4+ and CD8+ T cells and by natural killer cells. Here, we show that IFN-gamma is specifically displayed at a low concentration on the cell surface of those activated T cells from mouse and man which express IFN-gamma. It is transiently expressed on the cell surface with kinetics similar to those of intracellular IFN-gamma expression. Detectable surface IFN-gamma is not expressed by activated T helper (Th) cells producing other cytokines but which do not express IFN-gamma. Thus, surface IFN-gamma is the first available marker for live T lymphocytes expressing IFN-gamma, e.g. Th1 cells.
Assenmacher
M
M
Institute for Genetics, University of Cologne, Germany.
Scheffold
A
A
Schmitz
J
J
Segura Checa
J A
JA
Miltenyi
S
S
Radbruch
A
A
D000818:Animals; D006801:Humans; D007371:Interferon-gamma / Q000032:analysis* / Q000096:biosynthesis*; D015847:Interleukin-4 / Q000096:biosynthesis; D015848:Interleukin-5 / Q000096:biosynthesis; D007424:Intracellular Fluid / Q000737:chemistry; D008213:Lymphocyte Activation; D008565:Membrane Proteins / Q000032:analysis / Q000096:biosynthesis*; D051379:Mice; D008807:Mice, Inbred BALB C; D008822:Mice, Transgenic; D013601:T-Lymphocytes / Q000145:classification / Q000378:metabolism*
D015848:Interleukin-5; D008565:Membrane Proteins; D015847:Interleukin-4; D007371:Interferon-gamma