Establishment of an experimental model for continuous intraportal infusion of chemo/immunotherapeutic agents in the rat.
The results of adjuvant continuous intraportal chemotherapy in preventing hepatic metastasis, following curative colorectal surgery, are still equivocal. To improve the efficacy of this treatment we tried to establish an experimental model for continuous intraportal infusion in Wistar male rats. Using a drug infusion balloon catheter, which can be fixed on the back of the rat, the continuous intraportal administration of chemo/immunotherapeutic agents was carried out in 47 rats for 5 days. Of them, 23 received chemotherapeutic (Mitomycin C/5-fluorouracil) and 24 received chemoimmunotherapeutic (Mitomycin C/5-fluorouracil/Lentinan) agents. The overall success rate of complete 5-day infusion of the agents was 70.2%. The concentration of 5-fluorouracil in the peripheral blood ranged from 5ng/ml to 17ng/ml (mean 10.4ng/ml) and in the portal blood from 192ng/ml to 610ng/ml (mean 312.0ng/ml) following the perfect continuous infusion of the agents. It can thus be suggested that our established experimental model can be used for the study of continuous intraportal infusion in unrestrained rats.
Rafique
M
M
Department of Surgery, Shinshu University School of Medicine, Nagano-ken, Japan.
Adachi
W
W
Shiohara
E
E
D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage*; D015179:Colorectal Neoplasms / Q000188:drug therapy; D005472:Fluorouracil / Q000008:administration & dosage / Q000493:pharmacokinetics; D007167:Immunotherapy; D007262:Infusions, Intravenous; D008114:Liver Neoplasms, Experimental / Q000517:prevention & control* / Q000556:secondary*; D008297:Male; D011169:Portal Vein; D051381:Rats; D017208:Rats, Wistar
D000970:Antineoplastic Agents; D005472:Fluorouracil