Preparation and characterization of anti-peptide antibodies directed against human phenol and hydroxysteroid sulphotransferases.
Sulphotransferases (STs) catalyze the sulphation and, in general, detoxication of a large number of xenobiotics and endogenous compounds. A total of six synthetic peptides derived from the cDNA-derived amino acid sequences of the human phenol-sulphating form of phenosulphotransferase (P-PST) and human hydroxysteroid sulphotransferase (HST)--three from each sequence--were separately conjugated to the carrier protein keyhole limpet hemocyanin, and used to immunize rabbits. One successful antibody preparation was produced from among the P-PST peptides, and two from the HST peptides. On immunoblot analysis following SDS/PAGE, the anti-P-PST antibodies recognized two major forms of phenol ST in man, P-PST and the monoamine-sulphating form of PST, M-PST, and the two antibody preparations against HST recognized the human HST. These experiments demonstrate that it is possible to design specific antibodies against human sulphotransferases based on their amino acid sequences.
Sharp
S
S
Department of Biochemical Medicine, University of Dundee, Ninewells Hospital and Medical School, Scotland, U.K.
Coughtrie
M W
MW
Forbes
K J
KJ
Hume
R
R
D000276:Adjuvants, Immunologic / Q000378:metabolism; D000595:Amino Acid Sequence; D000906:Antibodies / Q000378:metabolism; D015239:Arylsulfotransferase / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000276:immunology; D018076:DNA, Complementary / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D006433:Hemocyanins / Q000276:immunology; D006801:Humans; D015151:Immunoblotting; D008969:Molecular Sequence Data; D010455:Peptides / Q000276:immunology*; D015238:Sulfotransferases / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000276:immunology
D000276:Adjuvants, Immunologic; D000906:Antibodies; D018076:DNA, Complementary; D010455:Peptides; D006433:Hemocyanins; D015238:Sulfotransferases; D015239:Arylsulfotransferase; C028152:alcohol sulfotransferase; C032808:keyhole-limpet hemocyanin