title: Importance of target innervation in recovery from axotomy-induced loss of androgen receptor in rat perineal motoneurons.
abstract: In adult male rats, axotomy of the spinal nucleus of the bulbocavernosus (SNB) motoneurons transiently down-regulates androgen receptor (AR) immunoreactivity. The present study investigates the importance of target reinnervation in the recovery of AR expression in axotomized SNB motoneurons after short (up to 5 days) and long (1 to 6 weeks) periods of recovery. In the long-term recovery experiment, animals were divided into two groups. In one, the two stumps of the cut pudendal nerve, which carries the axons of the SNB motoneurons, were sutured together immediately after axotomy. In the second group, the proximal stump was ligated immediately after axotomy to prevent target reinnervation. Axotomy of the SNB motoneurons caused a significant down-regulation in AR immunoreactivity within 3 days. At 6 weeks, AR immunoreactivity was still depressed in ligated animals but had recovered to control levels in resutured animals. The recovery in the resutured group was coincident with the first signs of reinnervation of the target perineal muscles, although reinnervation seemed to lag behind AR immunoreactivity. SNB soma size was significantly reduced 2 weeks after axotomy and returned to control levels after 6 weeks of recovery only in the resutured animals. These findings suggest that the target perineal muscles play a role in the regulation of AR expression and androgen sensitivity in the SNB motoneurons, perhaps mediated by muscle-derived trophic factors.
authors.affiliation: Department of Physiological Science, University of California, Los Angeles 90095-1527, USA.
authors.forename: H A
authors.initials: HA
authors.lastname: al-Shamma
authors.forename: A P
authors.initials: AP
authors.lastname: Arnold
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001369:Axons / Q000502:physiology*; D008297:Male; D009046:Motor Neurons / Q000378:metabolism*; D009469:Neuromuscular Junction / Q000502:physiology; D010413:Penis / Q000294:innervation*; D010502:Perineum / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011944:Receptors, Androgen / Q000378:metabolism*; D013116:Spinal Cord / Q000502:physiology*
chemical_list: D011944:Receptors, Androgen