Impaired release of cholecystokinin (CCK) from synaptosomes in old rats.
Cholecystokinin (CCK) is an abundant neurotransmitter peptide in the brain. CCK release from synaptosomes obtained from the cerebral cortex, the level of CCK mRNA and the tissue concentration of CCK were examined in young and old rats. CCK release stimulated by KCl was attenuated in old rats but that stimulated by calcium ionophore was comparable in animals at both ages. The CCK mRNA level in the cerebral cortex was decreased significantly in old rats despite the significant increase in CCK content. These results suggested that aging impaired CCK release, resulting in tissue accumulation and a decrease in the synthesis of CCK (the level of CCK mRNA).
Ohta
M
M
Department of Clinical Physiology, Tokyo Metropolitan Institute of Gerontology, Japan.
Tanaka
Y
Y
Masuda
M
M
Miyasaka
K
K
Funakoshi
A
A
D000367:Age Factors; D000375:Aging; D000818:Animals; D002540:Cerebral Cortex / Q000378:metabolism; D002766:Cholecystokinin / Q000378:metabolism*; D018076:DNA, Complementary; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar; D013574:Synaptosomes / Q000378:metabolism*
D018076:DNA, Complementary; D012333:RNA, Messenger; D002766:Cholecystokinin