Establishment of a human T-lymphoma cell line (H-TL90) and analysis of its biological characteristics.
We established a human T-lymphoma cell line from the cancerous ascites of a male patient with prostate cancer which was named H-TL90. This cell line was characterized by its histological features, and by chromosomal and immunological analysis. Immunophenotypic analysis revealed that the cells expressed surface antigen CD-3CD-4CD+7CD-8. Biological analysis revealed that the cell can promote lymphocyte proliferation. This suggested that the cell line has an autosecretion function. Cytogenetic analysis revealed that H-TL90 was a hyperdiploid with 47 chromosomes and had characteristic translocation between chromosome 3 and 11, and the deletion of the long arm of chromosome 6. These results demonstrated the H-TL90 cell line can be a useful model for the study of human T-lymphoma.
Shi
L
L
Department of Pathology, Haibin Medical University.
Liu
X
X
Zhang
Y
Y
Li
X
X
Li
D
D
Wang
W
W
D000328:Adult; D000818:Animals; D006801:Humans; D016399:Lymphoma, T-Cell / Q000473:pathology*; D008297:Male; D051379:Mice; D008819:Mice, Nude; D014407:Tumor Cells, Cultured