title: Anti-sense oligodeoxynucleoside phosphorothioates nonspecifically inhibit invasion of red blood cells by malaria parasites.
abstract: Anti-sense and sense oligodeoxynucleoside phosphorothioates, based on analysis of the secondary structure of Plasmodium falciparumdihydrofolate reductase-thymidylate synthase mRNA, were synthesized. Their effects on P. falciparum growth in vitro were examined by microscopy and [3H] hypoxanthine incorporation. Both anti-sense and sense oligodeoxynucleoside phosphorothioates inhibit invasion of red blood cells by merozoites and this is interpreted as being caused by their polyanionic nature. Specific anti-sense effects of the oligonucleoside phosphorothioates could not however be demonstrated.
authors.affiliation: Institute of Fundamental Studies, Kandy, Sri Lanka. Ranjan@ifs.ac.lk
authors.forename: R
authors.initials: R
authors.lastname: Ramasamy
authors.forename: R
authors.initials: R
authors.lastname: Kanagaratnam
authors.forename: K
authors.initials: K
authors.lastname: Misiura
authors.forename: G
authors.initials: G
authors.lastname: Rebowski
authors.forename: R
authors.initials: R
authors.lastname: Amerakoon
authors.forename: W J
authors.initials: WJ
authors.lastname: Stec
mesh_terms: D000818:Animals; D001483:Base Sequence; D017931:DNA Primers / Q000737:chemistry; D004912:Erythrocytes / Q000469:parasitology*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D008969:Molecular Sequence Data; D016376:Oligonucleotides, Antisense / Q000494:pharmacology*; D010963:Plasmodium falciparum / Q000254:growth & development*; D013762:Tetrahydrofolate Dehydrogenase / Q000235:genetics; D013873:Thionucleotides / Q000494:pharmacology*
chemical_list: D017931:DNA Primers; D016376:Oligonucleotides, Antisense; D013873:Thionucleotides; D013762:Tetrahydrofolate Dehydrogenase