A novel PRD I and TG binding activity involved in virus-induced transcription of IFN-A genes.
Comparative analysis of the inducible elements of the mouse interferon A4 and A11 gene promoters (IE-A4 and IE-A11) by transient transfection experiments, DNase 1 footprinting and electrophoretic mobility shift assays resulted in identification of a virus-induced binding activity suggested to be involved in NDV-induced activation of transcription of these genes. The virus-induced factor, termed VIF, is activated early by contact of virions with cells. It specifically recognizes the PRD I-like domain shared by both inducible elements, as well as the TG-like domain of IE-A4. This factor, distinct from the IRF-1, IRF-2 and the alpha F1 binding proteins and presenting a different affinity pattern from that of the TG protein, is proposed as a candidate for IFN-type I gene regulation.
Génin
P
P
Laboratoire de Régulation de l'Expression des Gènes Eucaryotes, CNRS, UPR 37, UFR Biomédicale des Saints-Pères, Université René Descartes, Paris, France.
Bragança
J
J
Darracq
N
N
Doly
J
J
Civas
A
A
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D002460:Cell Line; D004268:DNA-Binding Proteins / Q000235:genetics*; D016898:Interferon-alpha / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D009522:Newcastle disease virus; D000074462:Positive Regulatory Domain I-Binding Factor 1; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012045:Regulatory Sequences, Nucleic Acid; D012097:Repressor Proteins / Q000235:genetics*; D014157:Transcription Factors; D014158:Transcription, Genetic
D004268:DNA-Binding Proteins; D016898:Interferon-alpha; C513258:Prdm1 protein, mouse; D012097:Repressor Proteins; D014157:Transcription Factors; D000074462:Positive Regulatory Domain I-Binding Factor 1