title: Structural variation of the pseudoautosomal region between and within inbred mouse strains.
abstract: The pseudoautosomal region (PAR) is a segment of shared homology between the sex chromosomes. Here we report additional probes for this region of the mouse genome. Genetic and fluorescence in situ hybridization analyses indicate that one probe, PAR-4, hybridizes to the pseudoautosomal telomere and a minor locus at the telomere of chromosome 9 and that a PCR assay based on the PAR-4 sequence amplifies only the pseudoautosomal locus (DXYHgu1). The region detected by PAR-4 is structurally unstable; it shows polymorphism both between mouse strains and between animals of the same inbred strain, which implies an unusually high mutation rate. Variation occurs in the region adjacent to a (TTAGGG)n array. Two pseudoautosomal probes can also hybridize to the distal telomeres of chromosomes 9 and 13, and all three telomeres contain DXYMov15. The similarity between these telomeres may reflect ancestral telomere-telomere exchange.
authors.affiliation: Medical Research Council Human Genetics Unit, Western General Hospital, Edinburgh, United Kingdom.
authors.forename: D
authors.initials: D
authors.lastname: Kipling
authors.forename: H E
authors.initials: HE
authors.lastname: Wilson
authors.forename: E J
authors.initials: EJ
authors.lastname: Thomson
authors.forename: M
authors.initials: M
authors.lastname: Lee
authors.forename: J
authors.initials: J
authors.lastname: Perry
authors.forename: S
authors.initials: S
authors.lastname: Palmer
authors.forename: A
authors.initials: A
authors.lastname: Ashworth
authors.forename: H J
authors.initials: HJ
authors.lastname: Cooke
mesh_terms: D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D018244:Chromosomes, Artificial, Yeast; D003001:Cloning, Molecular; D017931:DNA Primers / Q000737:chemistry; D051379:Mice; D008815:Mice, Inbred Strains / Q000235:genetics*; D008969:Molecular Sequence Data; D012150:Polymorphism, Restriction Fragment Length; D016615:Telomere; D014960:X Chromosome; D014998:Y Chromosome
chemical_list: D017931:DNA Primers