title: Inhibition of NFkB DNA binding activity by glucocorticoids in rat brain.
abstract: The influence of glucocorticoids on the transcription factor NFkB was investigated by using the gel mobility shift assay with nuclear extracts prepared from rat cerebral cortex and hippocampus after a variety of in vivo treatments. Following stimulation with each of three treatments, kainate, pilocarpine, or lithium plus pilocarpine-induced seizures, NFkB DNA binding activity was significantly greater in the cortex and hippocampus from adrenalectomized than from adrenal-intact rats. These results indicate that in rat brain glucocorticoids inhibit NFkB activity in addition to the previously reported inhibition of the transcription factor AP-1 (activator protein 1). Impairment of stimulus-induced transcription factor activity may contribute to the deleterious effects of prolonged elevations of glucocorticoids on neuronal function.
authors.affiliation: Department of Psychiatry and Behavioral Neurobiology, University of Alabama at Birmingham 35294, USA.
authors.forename: T
authors.initials: T
authors.lastname: Unlap
authors.forename: R S
authors.initials: RS
authors.lastname: Jope
mesh_terms: D000315:Adrenalectomy; D000818:Animals; D001483:Base Sequence; D001923:Brain Chemistry / Q000187:drug effects / Q000502:physiology*; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism; D004247:DNA / Q000378:metabolism*; D018690:Excitatory Amino Acid Agonists / Q000494:pharmacology; D005938:Glucocorticoids / Q000502:physiology*; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism; D007608:Kainic Acid / Q000494:pharmacology; D008094:Lithium / Q000494:pharmacology; D008297:Male; D008969:Molecular Sequence Data; D018721:Muscarinic Agonists / Q000494:pharmacology; D016328:NF-kappa B / Q000096:biosynthesis*; D018384:Oxidative Stress; D010862:Pilocarpine / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D018808:Transcription Factor AP-1 / Q000378:metabolism
chemical_list: D018690:Excitatory Amino Acid Agonists; D005938:Glucocorticoids; D018721:Muscarinic Agonists; D016328:NF-kappa B; D018808:Transcription Factor AP-1; D010862:Pilocarpine; D004247:DNA; D008094:Lithium; D007608:Kainic Acid