title: Maturation of fiber membranes in the human eye lens. Ultrastructural and Raman microspectroscopic observations.
abstract: Electron microscopic, freeze fracture and cholesterol cytochemical studies have revealed that the maturation of lens fibers is accompanied by considerable changes in the ultrastructure of their limiting membranes. These changes are in line with biochemical studies on the cholesterol content in the lens and support the physiological notion that upon maturation lens membranes are transformed from normal permeable to nonleaky membranes. In addition it is shown that early cortical changes involved disturbances of lens membranes. As a consequence it can be postulated that, in order to be significant, future studies on the early events leading to cataractous disturbances must be carried out using a combination of histochemical, immunohistochemical and regional biochemical approaches.
authors.affiliation: Department of Morphology, The Netherlands Ophthalmic Research Institute, Amsterdam.
authors.forename: G F
authors.initials: GF
authors.lastname: Vrensen
authors.forename: H J
authors.initials: HJ
authors.lastname: Duindam
mesh_terms: D000900:Anti-Bacterial Agents / Q000378:metabolism; D002386:Cataract / Q000473:pathology*; D002462:Cell Membrane / Q000737:chemistry / Q000648:ultrastructure; D002784:Cholesterol / Q000032:analysis / Q000378:metabolism; D005372:Filipin / Q000378:metabolism; D005614:Freeze Fracturing; D006651:Histocytochemistry / Q000379:methods; D006801:Humans; D007908:Lens, Crystalline / Q000737:chemistry / Q000648:ultrastructure*; D008855:Microscopy, Electron, Scanning; D013059:Spectrum Analysis, Raman
chemical_list: D000900:Anti-Bacterial Agents; D005372:Filipin; D002784:Cholesterol