In vivo comparative study of two lactobionate based solutions for prolonged heart preservation.
The duration of safe heart preservation must be improved. Using a heterotopic heart transplantation model, we compared in vivo the recovery of rabbits hearts preserved with a K+Lactobionate based fluid (UW: University of Wisconsin solution) or with a Na+Lactobionate based fluid. In the "preservation" group, hearts were cold stored (4 degrees C) for 6 hours with UW (n = 9) or Na+Lactobionate solution (n = 9). In the "transplantation" group, cold storage was followed by 3 hours of reperfusion (UW: n = 8, Na+Lactobionate solution: n = 7). Functional recovery, adenine nucleotide pool, circulating blood cardiac enzymes, circulating blood and tissue malondialdehyde (MDA) were measured. Left ventricular end-diastolic and developed pressures at different preload levels were better after preservation with UW than with Na+Lactobionate solution (p < 0.05). Also with UW, adenosine diphosphate and total adenine nucleotide content were significantly higher than with Na+Lactobionate solution (p < 0.05) whereas adenosine triphosphate, monophosphate and energy charges were similar. Cardiac enzymes and tissue MDA were similar with UW and Na+Lactobionate solution. In circulating blood, MDA was not detected. These results enhance the superiority of UW solution over a Na+Lactobionate based solution for long term heart preservation.
Baufreton
C
C
Centre de Recherches Chirurgicales Henri Mondor, C.N.R.S. URA 1413, Créteil-France.
Charloux
C
C
Jaffres
P
P
Paul
M
M
Roudot-Thoraval
F
F
Perennec
J
J
Astier
A
A
Loisance
D Y
DY
D000227:Adenine Nucleotides / Q000378:metabolism; D000818:Animals; D002304:Cardiac Pacing, Artificial; D003402:Creatine Kinase / Q000378:metabolism; D004187:Disaccharides; D004573:Electrolytes / Q000378:metabolism; D006321:Heart / Q000187:drug effects / Q000502:physiology; D016027:Heart Transplantation; D006439:Hemodynamics / Q000502:physiology; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008315:Malondialdehyde / Q000378:metabolism; D009206:Myocardium / Q000201:enzymology / Q000378:metabolism; D009926:Organ Preservation; D011817:Rabbits; D012996:Solutions
D000227:Adenine Nucleotides; D004187:Disaccharides; D004573:Electrolytes; D012996:Solutions; D008315:Malondialdehyde; C005608:lactobionic acid; D007770:L-Lactate Dehydrogenase; D003402:Creatine Kinase