title: [Site-specific endonuclease and methylase from thermophilic bacteria of Bacillus species IS4].
abstract: The site-specific endonuclease R . BspIS4I and methylase M . BspIS4I have been isolated and purified to functional purity from the thermophilic strain of Bacillus species IS4. R . BspIS4I recognizes sequence [sequence: see text] on the DNA and cleaves it as indicated by the arrows to form single-stranded 4-nucleotide 5'-protruding termini. The enzyme is an isoschizomer of BbvII. M . BspIS4I is related to adenine-specific methylase.
authors.forename: N V
authors.initials: NV
authors.lastname: Zelinskaia
authors.forename: N P
authors.initials: NP
authors.lastname: Kovalevskaia
authors.forename: N N
authors.initials: NN
authors.lastname: Matvienko
authors.forename: L A
authors.initials: LA
authors.lastname: Zheleznaia
authors.forename: N I
authors.initials: NI
authors.lastname: Matvienko
mesh_terms: D001407:Bacillus / Q000201:enzymology*; D001483:Base Sequence; D002852:Chromatography, Ion Exchange; D004269:DNA, Bacterial / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D008969:Molecular Sequence Data; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific) / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D004269:DNA, Bacterial; C097868:DNA modification methylase BspIS4I; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific)
vernacular_title: Saĭt-spetsificheskie éndonukleaza i metilaza iz termofil'noĭ bakterii Bacillus species IS4.