Microdialysis measurement of intracerebral somatostatin in the goat.
A microdialysis sampling technique for the intracerebral measurement of somatostatin (SS) in extracellular fluid was examined in the goat. The microdialysis probe (70-mm shaft, 0.5 mm outer diameter) contained at its tip a 4-mm length of copolymer dialysis membrane (20 kDa cut-off). Artificial cerebrospinal fluid (artificial CSF) was pumped through the probe tip at a rate of 4 microliters/min with a batter-driven syringe pump, and effluent fractions of dialysate (120 microliters) were collected every 30 min. An in vitro recovery test showed that changes in the SS concentration in dialysate were highly correlated (r = 0.95, P < 0.01) with those in the external medium, and the relative recovery averaged 2.0%. As a validation for in vivo microdialysis, trails were conducted with conscious behaving goats wherein the inflow dialysate was changed transiently from artificial CSF with low potassium (2.5 mM) to a solution of 300 mM KCl. Potassium-induced depolarization around the probe tip located in the preoptic area and in the hypothalamus induced an increase in SS concentrations in dialysate at each location. In the most remarkable response, the concentrations of SS were increased 6-fold and 11-fold in the first and second 30-min fractions, respectively, compared with prepotassium concentrations. These results suggest that intracerebral SS levels in extracellular fluid could be estimated from conscious behaving goats by the use of our intracerebral microdialysis system.
Hashizume
T
T
Laboratory of Animal Breeding and Reproduction, Faculty of Agriculture, Iwate University, Morioka, Japan.
Nitta
Y
Y
Kasuya
K
K
Kanematsu
S
S
D000818:Animals; D005260:Female; D006041:Goats / Q000378:metabolism*; D007031:Hypothalamus / Q000737:chemistry* / Q000378:metabolism / Q000502:physiology; D017551:Microdialysis / Q000379:methods / Q000592:standards / Q000662:veterinary*; D011188:Potassium / Q000494:pharmacology; D011301:Preoptic Area / Q000737:chemistry* / Q000378:metabolism / Q000502:physiology; D011863:Radioimmunoassay / Q000662:veterinary; D013004:Somatostatin / Q000032:analysis* / Q000378:metabolism
D013004:Somatostatin; D011188:Potassium