Energy-dispersive X-ray microanalysis of calcified tissues after NaOCl treatment and/or resin embedding.
By energy-dispersive X-ray microanalysis (EDX) in scanning electron microscopy, we studied the calcification of the inner-basic lamellas of a cow bone and the coronal cementum of a horse tooth treated with sodium hypochlorite (NaOCl). These tissues were divided into 4 groups with a combination of NaOCl treatment and polyester-resin embedding including, [A]: non-NaOCl + Resin, [B]: non-NaOCl + non-resin, [C]: NaOCl + Resin, and [D]: NaOCl + non-Resin. From the Ca and P values by EDX analysis, it was suggested that the natural porous spaces of [B] were higher than those of [A], and both the natural and NaOCl-soluble porous spaces were highest in [D]. However, [A] had the lowest porous spaces in both the tissues because the micropores formed 3-dimensionally by NaOCl treatment might be incompletely filled with the resin. The backscattered electron microscopy and the difference of the Ca/P ratios indicated that the NaOCl treatment of the calcified tissues caused some minerals besides organic materials to dissolve. Thus, the sample preparation of [B] is suitable for the quantitative EDX of calcified tissues, whereas the data of [C] except for the Ca/P ratio may be used to approximate the Ca and P contents.
Kodaka
T
T
Department of Oral Anatomy, School of Dentistry, Showa University, Tokyo, Japan.
Mori
R
R
Sano
T
T
Debari
K
K
D000818:Animals; D001641:Bicuspid / Q000737:chemistry* / Q000187:drug effects; D002118:Calcium / Q000032:analysis*; D002417:Cattle; D003739:Dental Cementum / Q000737:chemistry / Q000187:drug effects; D004577:Electron Probe Microanalysis / Q000379:methods*; D006736:Horses; D006801:Humans; D008663:Metacarpus / Q000737:chemistry* / Q000187:drug effects; D008855:Microscopy, Electron, Scanning; D012973:Sodium Hypochlorite / Q000494:pharmacology; D013045:Species Specificity; D016610:Tissue Embedding / Q000379:methods; D014965:X-Rays
D012973:Sodium Hypochlorite; D002118:Calcium