Protective immunity to Shiga-like toxin I following oral immunization with Shiga-like toxin I B-subunit-producing Vibrio cholerae CVD 103-HgR.
This study addresses a mechanism for inducing systemic immunity to Shiga-like toxins by oral administration of a Shiga-like toxin I B-subunit-expressing Vibrio cholerae vaccine strain [CVD 103-HgR(pDA60)]. Two sets of three rabbits were given either CVD 103-HgR or CVD 103-HgR(pDA60) orally. All rabbits immunized with CVD 103-HgR(pDA60) developed neutralizing serum antibodies to Shiga-like toxin I. None of the controls developed such antibodies.
Acheson
D W
DW
Division of Geographic Medicine and Infectious Diseases, New England Medical Center, Boston, Massachusetts 02111, USA.
Levine
M M
MM
Kaper
J B
JB
Keusch
G T
GT
D000284:Administration, Oral; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001427:Bacterial Toxins / Q000276:immunology* / Q000627:therapeutic use*; D022121:Cholera Vaccines / Q000008:administration & dosage / Q000627:therapeutic use; D004927:Escherichia coli Infections / Q000517:prevention & control*; D008297:Male; D009500:Neutralization Tests; D011817:Rabbits; D022622:Shiga Toxin 1; D014611:Vaccination; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000627:therapeutic use
D000907:Antibodies, Bacterial; D001427:Bacterial Toxins; D022121:Cholera Vaccines; D022622:Shiga Toxin 1; D014614:Vaccines, Synthetic