Use of tissue slices in chemical mixture toxicology and interspecies investigations.
Precision-cut tissue slices have proven to be a useful in vitro system for biotransformation and toxicity studies. Since tissue slices can be readily prepared from a variety of tissues and species, they can easily be used for interspecies investigations and comparisons. Furthermore, slices can be readily prepared from human tissue, thus comparisons (extrapolation) can be made between laboratory animals and humans. Slices can also be used to examine the toxic interactions of chemicals in vitro. It is important to use the correct experimental design to demonstrate toxic interactions and to assure that the tissue slices are properly exposed to the chemicals. Overall, tissue slices offer a valid in vitro system for performing species comparisons and chemical-chemical interaction studies.
Gandolfi
A J
AJ
Department of Pharmacology, University of Arizona, Tucson 85724, USA.
Brendel
K
K
Fisher
R L
RL
Michaud
J P
JP
D000818:Animals; D001711:Biotransformation; D001969:Bromobenzenes / Q000633:toxicity; D002722:Chlorobenzenes / Q000633:toxicity; D004347:Drug Interactions; D015386:Hazardous Substances / Q000378:metabolism / Q000633:toxicity*; D006801:Humans; D066298:In Vitro Techniques; D007424:Intracellular Fluid / Q000737:chemistry; D008099:Liver / Q000473:pathology; D008867:Microtomy; D011188:Potassium / Q000032:analysis; D051381:Rats; D011916:Rats, Inbred F344; D017207:Rats, Sprague-Dawley; D013045:Species Specificity; D015262:Xenobiotics / Q000378:metabolism / Q000633:toxicity*
D001969:Bromobenzenes; D002722:Chlorobenzenes; D015386:Hazardous Substances; D015262:Xenobiotics; D011188:Potassium