Use of capillary zone electrophoresis in an investigation of peptide uptake by dairy starter bacteria.
A capillary zone electrophoresis (CZE) method to separate the peptide series val-glyn, where n is 1 to 4, has been evaluated and compared to separation by reversed-phase high-performance liquid chromatography (RP-HPLC). The method was able to quantitate peptides present a very low concentrations (down to 0.05 mM) with high reproducibility and accuracy and was capable of separating peptides differing in size by only a single glycine residue. It could also separate the peptides val-gly and leu-gly which differed in only a single side-chain methylene group. The method was fast, required small sample volumes, and proved to be superior to RP-HPLC. The suitability of the CZE method to analyze peptide uptake by dairy starter bacteria is discussed.
Moore
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IL
Department of Chemistry and Biochemistry, Massey University, Palmerston North, New Zealand.
Pritchard
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GG
Otter
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DE
D002851:Chromatography, High Pressure Liquid; D003611:Dairy Products / Q000382:microbiology*; D019075:Electrophoresis, Capillary; D007700:Kinetics; D013294:Lactococcus lactis / Q000378:metabolism*; D010455:Peptides / Q000032:analysis* / Q000378:metabolism; D014269:Trifluoroacetic Acid / Q000494:pharmacology
D010455:Peptides; D014269:Trifluoroacetic Acid