The effect of N-acetylaspartate on the intracellular free calcium concentration in NTera2-neurons.
We have studied the effect of relatively high concentrations of extracellular N-acetylaspartate (NAA) on the intracellular free calcium concentration [Ca2+]i in NTera2-neurons. While low concentrations of extracellular NAA (0.1, 1 mM) had no effect on the [Ca2+]i, high concentrations of extracellular NAA (3, 10 mM) elicited sharp and statistically significant elevations of [Ca2+]i. Different classes of antagonists of the N-methyl-D-aspartate (NMDA) receptor abolished the NAA induced elevations of the [Ca2+]i, indicating the involvement of the NMDA receptor in NAA-induced elevations of [Ca2+]i.
Rubin
Y
Y
Radiology Department, Hospital of the University of Pennsylvania, Philadelphia 19104, USA.
LaPlaca
M C
MC
Smith
D H
DH
Thibault
L E
LE
Lenkinski
R E
RE
D000704:Analysis of Variance; D000818:Animals; D001224:Aspartic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D016291:Dizocilpine Maleate / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D009474:Neurons / Q000187:drug effects*; D016194:Receptors, N-Methyl-D-Aspartate / Q000187:drug effects; D013997:Time Factors
D016194:Receptors, N-Methyl-D-Aspartate; D001224:Aspartic Acid; D016291:Dizocilpine Maleate; C000179:N-acetylaspartate; D002118:Calcium