The 5' coding sequence of IL-2 receptor alpha chain mRNA mediates mRNA stabilization by HTLV-1 Rex.
The interleukin-2 receptor alpha chain (IL-2R alpha) is a T-cell growth factor receptor and is known to be induced in helper T cells by infection with human T-cell leukaemia virus type-1 (HTLV-1). The Rex protein of HTLV-1 has been shown to stabilize IL-2R alpha mRNA. Although the 3' untranslated region of many RNA has been regarded as a key element for stabilization, we found that the first 300 bases of the IL-2R alpha protein coding sequence were necessary for stabilization of the mRNA. As the first 201 bases were not sufficient for this effect, we conclude that the bases at position 201-300 downstream of the AUG start are important for stabilization.
Kanamori
H
H
Third Department of Internal Medicine, University of Tokyo, Japan.
Kodama
T
T
Yazaki
Y
Y
D018929:Cell Culture Techniques; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D016370:Gene Products, rex / Q000235:genetics*; D015368:Human T-lymphotropic virus 1 / Q000235:genetics*; D006801:Humans; D010957:Plasmids; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics*; D015375:Receptors, Interleukin-2 / Q000235:genetics*; D014162:Transfection
D016370:Gene Products, rex; D012333:RNA, Messenger; D015375:Receptors, Interleukin-2; D015500:Chloramphenicol O-Acetyltransferase