title: A rapid and simple ABO genotype screening method using a novel B/O2 versus A/O2 discriminating nucleotide substitution at the ABO locus.
abstract: An ABO genotype screening method discriminating the common alleles A1, A2, B, O1 and O2 at the ABO locus was made possible by the discovery of a novel nucleotide substitution (G1096A) present only in B and O2 alleles. A rapid and reliable single-tube approach using multiplex PCR with four primers amplifying exons 6 and 7 of the ABO genes followed by simultaneous addition of two restriction enzymes was developed and validated in a population of 150 Swedish blood donors. This technique is the most cost-efficient and informative ABO genotyping method reported to date.
authors.affiliation: Blood Centre, University Hospital, Lund, Sweden.
authors.forename: M L
authors.initials: ML
authors.lastname: Olsson
authors.forename: M A
authors.initials: MA
authors.lastname: Chester
mesh_terms: D000017:ABO Blood-Group System / Q000235:genetics*; D000483:Alleles; D001483:Base Sequence; D001782:Blood Donors; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D005820:Genetic Testing; D005838:Genotype; D006801:Humans; D008969:Molecular Sequence Data; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D015203:Reproducibility of Results; D013997:Time Factors
chemical_list: D000017:ABO Blood-Group System