[Substrate properties of dioxolane analogs of 3'-deoxythymidine-5'-triphosphate in DNA synthesis reactions, catalyzed by various DNA polymerases].
The substrate properties of 3'-deoxythymidine 5'-triphosphate analogs prepared on the basis of 2,4-disubstituted 1,3-dioxolanes were investigated in reactions of the DNA synthesis catalyzed by various DNA polymerases. The 4'-triphosphates of (+/-)-cis-4-hydroxymethyl-2-(1-thyminylmethyl)-1,3-dioxolane and the corresponding (+/-)-trans-isomer were shown to be terminating substrates of terminal deoxynucleotidyl transferase. 4'-Triphosphate of (+/-)-cis-4-hydroxymethyl-2-(1-thyminylmethyl)- 1,3-dioxolane terminates the DNA synthesis catalyzed by HIV reverse transcriptase, whereas 2'-triphosphate of (+/-)-cis-2-hydromethyl-4-(1-thyminylmethyl)-1,3-dioxolane is a terminator in the DNA synthesis catalyzed by HIV reverse transcriptase and the Klenow fragment of DNA polymerase I.
Efimtseva
E V
EV
Mikhaĭlov
S N
SN
Viktorova
L S
LS
Rozovskaia
T A
TA
Bibilashvili
R Sh
RSh
D001483:Base Sequence; D002384:Catalysis; D004256:DNA Polymerase I / Q000378:metabolism*; D004148:Dioxolanes / Q000737:chemistry*; D006678:HIV / Q000201:enzymology; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D013379:Substrate Specificity; D013942:Thymine Nucleotides / Q000737:chemistry / Q000378:metabolism*
D004148:Dioxolanes; D013942:Thymine Nucleotides; D012194:RNA-Directed DNA Polymerase; D004256:DNA Polymerase I; C024157:thymidine 5'-triphosphate
Substratnye svoĭstva dioksolanovykh analogov 3'-dezoksitimidin-5'-trifosfata v reaktsiiakh sinteza DNK, kataliziruemykh razlichnymi DNK-polimerazami.