title: Transformation of Synechococcus with a gene for choline oxidase enhances tolerance to salt stress.
abstract: Choline oxidase, isolated from the soil bacterium Arthrobacter globiformis, converts choline to glycinebetaine (N-trimethylglycine) without a requirement for any cofactors. The gene for this enzyme, designated codA, was cloned and introduced into the cyanobacterium Synechococcus sp. PCC 7942. The codA gene was expressed under the control of a strong constitutive promoter, and the transformed cells accumulated glycinebetaine at intracellular levels of 60-80 mM. Consequently the cells acquired tolerance to salt stress, as evaluated in terms of growth, accumulation of chlorophyll and photosynthetic activity.
authors.affiliation: Department of Regulation Biology, National Institute for Basic Biology, Okazaki, Japan.
authors.forename: P
authors.initials: P
authors.lastname: Deshnium
authors.forename: D A
authors.initials: DA
authors.lastname: Los
authors.forename: H
authors.initials: H
authors.lastname: Hayashi
authors.forename: L
authors.initials: L
authors.lastname: Mustardy
authors.forename: N
authors.initials: N
authors.lastname: Murata
mesh_terms: D000222:Adaptation, Physiological; D000595:Amino Acid Sequence; D001173:Arthrobacter / Q000235:genetics; D001483:Base Sequence; D001622:Betaine / Q000378:metabolism; D002876:Chromosomes, Bacterial; D003001:Cloning, Molecular; D000458:Cyanobacteria / Q000201:enzymology / Q000235:genetics* / Q000502:physiology; D004269:DNA, Bacterial; D008969:Molecular Sequence Data; D009994:Osmolar Concentration; D017386:Sequence Homology, Amino Acid; D012965:Sodium Chloride; D014170:Transformation, Genetic
chemical_list: D004269:DNA, Bacterial; D001622:Betaine; D012965:Sodium Chloride