Chromosome-encoded inducible copper resistance in Pseudomonas strains.
Nine Pseudomonas strains were selected by their high copper tolerance from a population of bacteria isolated from heavy-metal polluted zones. Copper resistance (Cu(r)) was inducible by previous exposure of cultures to subinhibitory amounts of copper sulfate. All nine strains possessed large plasmids, but transformation and curing results suggest that Cu(r) is conferred by chromosomal genes. Plasmid-less Pseudomonas aeruginosa PAO-derived strains showed the same level of Cu(r) as environmental isolates and their resistance to copper was also inducible. Total DNA from the environmental Pseudomonas, as well as from P. aeruginosa PAO strains, showed homology to a Cu(r) P. syringae cop probe at low-stringency conditions but failed to hybridize at high-stringency conditions.
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Instituto de Investigaciones Químico-Biológicas, Universidad Michoacana, Morelia, México.
Gutiérrez
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Ambriz
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Cervantes
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D002876:Chromosomes, Bacterial; D003300:Copper / Q000494:pharmacology*; D019327:Copper Sulfate; D004269:DNA, Bacterial / Q000235:genetics; D004352:Drug Resistance, Microbial / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D010957:Plasmids; D011549:Pseudomonas / Q000145:classification / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000494:pharmacology; D014871:Water Microbiology; D014874:Water Pollutants, Chemical / Q000494:pharmacology
D004269:DNA, Bacterial; D012989:Soil Pollutants; D014874:Water Pollutants, Chemical; D003300:Copper; D019327:Copper Sulfate