A model for the incorporation of intracytoplasmic sperm injection into a private practice in vitro fertilization program.
OBJECTIVE
To describe a method for the training of personnel and the implementation of intracytoplasmic sperm injection (ICSI) into an IVF program. The results of the first 75 cycles are reviewed.


METHODS
Retrospective review of the first 75 consecutive ICSI procedures.


METHODS
Private, community-based IVF program.


METHODS
The fertilization rate, damage rate, ongoing pregnancy rate (PR), and implantation rate were measured.


RESULTS
Nine percent of the injected oocytes were damaged. The fertilization rate was 60%, and the cleavage rate was 98%. Fifty-nine percent of the cycles resulted in an ongoing pregnancy, and the implantation rate per embryo was 26%.


CONCLUSIONS
A high initial PR can be obtained with ICSI using a systematic training regimen.
Schoolcraft
W B
WB
Center for Reproductive Medicine, Englewood, Colorado, USA.
Schlenker
T
T
Adler
A
A
Alikani
M
M
D000328:Adult; D003593:Cytoplasm; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D006801:Humans; D007267:Injections; D008297:Male; D009865:Oocytes / Q000166:cytology; D011247:Pregnancy / Q000706:statistics & numerical data; D011331:Private Practice; D012189:Retrospective Studies; D013094:Spermatozoa