title: Study of the new stability properties induced by amino acid replacement of tyrosine 64 in cytochrome C553 from Desulfovibrio vulgaris Hildenborough using electrospray ionization mass spectrometry.
abstract: Hydrogen/deuterium exchange as well as charge state distribution monitored by electrospray ionization mass spectrometry were demonstrated to be a powerful and effective new tool for probing conformational properties of proteins in solution. In this paper, the influence of single amino acid replacements on the global conformation of cytochrome C553 from Desulfovibrio vulgaris Hildenborough using isotopic exchange monitored by electrospray ionization mass spectrometry is reported. Based on their respective charge state distributions and isotopic exchanges, we have differentiated relative stability of mutants and a ladder classification with the order being wild-type > Y64F = Y64L > Y64V > Y64A, under specific conditions of pH, is proposed.
authors.affiliation: Institut de Biologie Structurale Jean-Pierre Ebel, CEA/CNRS, Grenoble, France.
authors.forename: P
authors.initials: P
authors.lastname: Guy
authors.forename: H
authors.initials: H
authors.lastname: Rémigy
authors.forename: M
authors.initials: M
authors.lastname: Jaquinod
authors.forename: B
authors.initials: B
authors.lastname: Bersch
authors.forename: L
authors.initials: L
authors.lastname: Blanchard
authors.forename: A
authors.initials: A
authors.lastname: Dolla
authors.forename: E
authors.initials: E
authors.lastname: Forest
mesh_terms: D000595:Amino Acid Sequence; D003574:Cytochrome c Group / Q000737:chemistry* / Q000378:metabolism*; D016969:Desulfovibrio vulgaris / Q000378:metabolism*; D004355:Drug Stability; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D013058:Mass Spectrometry; D016297:Mutagenesis, Site-Directed; D017354:Point Mutation; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D014443:Tyrosine
chemical_list: D003574:Cytochrome c Group; D011994:Recombinant Proteins; C083951:cytochrome c553; D014443:Tyrosine