Expression of the Bacillus subtilis sacB gene confers sucrose sensitivity on mycobacteria.
Expression in mycobacteria of the structural gene sacB, which encodes the Bacillus subtilis levansucrase, was investigated. sacB expression is lethal to Mycobacterium smegmatis and Mycobacterium bovis BCG in the presence of 10% sucrose. sacB could thus be used as a counterselectable marker in mycobacteria.
Pelicic
V
V
Unité de Génétique Mycobactérienne, Centre National de la Recherche Scientifique URA 1300, Institut Pasteur, Paris, France. vpelicic@pasteur.fr
Reyrat
J M
JM
Gicquel
B
B
D001412:Bacillus subtilis / Q000201:enzymology / Q000235:genetics*; D005798:Genes, Bacterial; D005819:Genetic Markers; D006602:Hexosyltransferases / Q000235:genetics*; D008826:Microbial Sensitivity Tests; D009161:Mycobacterium / Q000187:drug effects* / Q000235:genetics; D009163:Mycobacterium bovis / Q000187:drug effects / Q000235:genetics; D011994:Recombinant Proteins; D012641:Selection, Genetic; D013395:Sucrose / Q000494:pharmacology*
D005819:Genetic Markers; D011994:Recombinant Proteins; D013395:Sucrose; D006602:Hexosyltransferases; C021513:levansucrase