The beta chain of the GPIIb molecule on ruminant leukocytes and platelets is not labelled by the sulfo-NHS-biotin method.
Glycoprotein (Gp)IIb/IIIa molecules have been immunoprecipitated from platelets and leukocytes of cattle, goat, horse, human, sheep and swine using specific monoclonal antibodies. The sulfo-NHS-biotin (sulfosuccinimidobiotin) method used to label the proteins has been found unsuitable for labelling the beta chain of the ruminant GPIIb/IIIa molecule. The beta chain was present on ruminant leukocytes and platelets when immunoprecipitates were silver stained.
Pintado
C O
CO
Departamento de Genética, Facultad de Veterinaria, Universidad de Córdoba, España.
Moreno
A
A
Barbancho
M
M
Pérez de la Lastra
J M
JM
Llanes
D
D
D000818:Animals; D001710:Biotin / Q000031:analogs & derivatives; D001792:Blood Platelets / Q000737:chemistry*; D006801:Humans; D007962:Leukocytes / Q000737:chemistry*; D010446:Peptide Fragments / Q000737:chemistry*; D019039:Platelet Glycoprotein GPIIb-IIIa Complex / Q000737:chemistry*; D011233:Precipitin Tests; D012418:Ruminants / Q000097:blood*; D016622:Silver Staining; D013388:Succinimides
D010446:Peptide Fragments; D019039:Platelet Glycoprotein GPIIb-IIIa Complex; D013388:Succinimides; C053827:biotinyl-N-hydroxysulfosuccinimide ester; D001710:Biotin