Finding prospective partners in the library: the two-hybrid system and phage display find a match.
The two-hybrid system uses the efficacy of yeast genetic assays to identify protein-protein interactions. It permits the rapid cloning of genes encoding products that interact with a given protein of interest. Also being developed are phage display methods that allow direct physical selection of binding proteins. These methods have significantly altered strategies for analysing signaling and regulatory pathways.
Allen
J B
JB
Howard Hughes Medical Institute, Houston, TX, USA.
Walberg
M W
MW
Edwards
M C
MC
Elledge
S J
SJ
D001435:Bacteriophages / Q000235:genetics; D001665:Binding Sites; D004247:DNA / Q000378:metabolism*; D015723:Gene Library; D005821:Genetic Techniques; D011993:Recombinant Fusion Proteins; D012441:Saccharomyces cerevisiae / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics*
D011993:Recombinant Fusion Proteins; D014157:Transcription Factors; D004247:DNA