DNA adducts in urothelial cells: relationship with biomarkers of exposure to arylamines and polycyclic aromatic hydrocarbons from tobacco smoke.
Markers of exposure to polycyclic aromatic hydrocarbons (urinary 1-hydroxypyrene-glucuronide) and aromatic amines (4-aminobiphenyl-hemoglobin adducts), as well as urinary mutagenicity, were measured in 47 healthy smokers and 50 nonsmokers. DNA adducts were determined by P32-postlabeling in the exfoliated bladder cells of 39 healthy subjects. Both 1-hydroxypyrene-glucuronide (1-OHPG) and 4-aminobiphenyl adducts (4-ABP-Hb) were associated with smoking habits, but only 4-ABP-Hb adducts were associated with consumption of black, air-cured tobacco. The levels of 2 DNA adducts (numbers 2 and 4) in urothelial cells were clearly associated with 4-ABP-Hb adducts, in all subjects and in smokers. Levels of one of these DNA adducts (number 2) were also associated with 1-hydroxypyrene-glucuronide in urines, but in smokers the association was not statistically significant. Overall, these observations constitute further evidence of a role of arylamines in tobacco-induced bladder cancer.
Vineis
P
P
Dipartimento di Scienze Biomediche e Oncologia Umana, University and Main Hospital, Turin, Italy.
Talaska
G
G
Malaveille
C
C
Bartsch
H
H
Martone
T
T
Sithisarankul
P
P
Strickland
P
P
D000611:Aminobiphenyl Compounds / Q000009:adverse effects*; D003367:Cotinine / Q000652:urine; D018736:DNA Adducts / Q000096:biosynthesis* / Q000652:urine; D006801:Humans; D008297:Male; D008875:Middle Aged; D009538:Nicotine / Q000652:urine; D011721:Pyrenes / Q000009:adverse effects*; D012907:Smoking / Q000009:adverse effects; D001743:Urinary Bladder / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism
D000611:Aminobiphenyl Compounds; D018736:DNA Adducts; D011721:Pyrenes; D009538:Nicotine; C030984:pyrene; D003367:Cotinine