Inflammatory properties of bile from dogs with pigment gallstones.
BACKGROUND
Gallbladder inflammation and mucus hypersecretion are prominent features of cholesterol and pigment gallstones in humans and animals. The factors leading to inflammation and mucus hypersecretion are poorly understood. These studies examine the inflammatory potential of bile from dogs with pigment gallstones.


METHODS
Dogs fed a methionine-deficient diet that produces pigment gallstones by 6 weeks were compared to normal dogs. Mucus layer thickness, myeloperoxidase activity, and interleukin-1-like activities were measured in canine gallbladder. The inflammatory potential of canine bile was determined by measuring mucus layer thickness, sodium absorption, myeloperoxidase activity and interleukin-1-like activity in guinea pig gallbladders exposed to normal and lithogenic canine bile for 4 hours.


RESULTS
Mean mucus layer thickness, myeloperoxidase, and interleukin-1 activity were significantly greater in canine gallbladders containing pigment gallstones. Bile from dogs with pigment gallstones markedly increased mucus layer thickness, myeloperoxidase activity, and interleukin-1 activity and decreased sodium absorption in normal guinea pig gallbladder. These effects were not eliminated by centrifuging bile to remove crystals and gallstones.


CONCLUSIONS
Canine bile from dogs with pigment gallstones contains soluble factors capable of causing inflammation in the gallbladder wall.
Rege
R V
RV
Department of Surgery, Veterans' Administration Lakeside Hospital, Chicago, Illinois, USA.
Prystowsky
J B
JB
D000818:Animals; D001646:Bile / Q000502:physiology*; D001654:Bile Pigments; D002764:Cholecystitis / Q000209:etiology*; D002769:Cholelithiasis / Q000378:metabolism*; D004285:Dogs; D005260:Female; D006168:Guinea Pigs; D007375:Interleukin-1 / Q000032:analysis; D009093:Mucus / Q000737:chemistry; D009195:Peroxidase / Q000032:analysis; D012964:Sodium / Q000378:metabolism
D001654:Bile Pigments; D007375:Interleukin-1; D012964:Sodium; D009195:Peroxidase