title: Replication of infectious laryngotracheitis virus in a quail cell line, QT-35.
abstract: The relative resistance of the quail fibroblastic cell line QT-35 to infection by infectious laryngotracheitis virus (ILTV) was circumvented by the continual passaging of infected cells in the presence of uninfected monolayers. Such virus-containing cells were used to extend the infection to an otherwise refractory quail liver cell line IQ1A, as well as to a normally permissive chicken liver cell line LMH, with nearly equal efficiency. In contrast, extracellular virus that had been derived from either QT-35 or IQ1A cells could not readily infect either quail cell line, although LMH cells were still susceptible. Therefore, the block to ILTV replication in quail cells is probably at the adsorption/penetration stage.
authors.affiliation: Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Illinois, Urbana 61801, USA.
authors.forename: W M
authors.initials: WM
authors.lastname: Schnitzlein
authors.forename: D N
authors.initials: DN
authors.lastname: Tripathy
mesh_terms: D000818:Animals; D002460:Cell Line; D003370:Coturnix; D005347:Fibroblasts / Q000821:virology; D001352:Herpesvirus 1, Gallid / Q000502:physiology*; D014779:Virus Replication