Comparison of [125I]iodomelatonin binding sites in infant cerebellum of sudden infant death syndrome and non-sudden infant death syndrome.
[125I]Iodomelatonin bindings sites in human infant cerebellum were studied by radioligand receptor binding assay and in vitro quantitative autoradiography. The binding sites characterized in membrane preparations revealed saturable, reversible and highly specific binding sites with a Kd value of 21.2 +/- 8.33 pM and a Bmax value of 2.02 +/- 0.52 fmol/mg protein. 10 microM of GTP gamma S significantly reduced the binding capacity, suggesting the possible G-protein coupling of the binding sites. Autoradiographic study showed that the labelling was mainly located in the cerebellar cortex. On comparison of the binding parameters from cerebellum of sudden infant death syndrome (SIDS) and non-SIDS infants, no significant change in binding capacity and binding affinity was detected. These findings suggest that the binding sites in cerebellum may not be related to the etiology of SIDS.
Yuan
H
H
Clarke Institute of Psychiatry, University of Toronto, Department of Psychiatry, Ontario, Canada.
Chan
C W
CW
Sturner
W Q
WQ
Pang
S F
SF
Brown
G M
GM
D000704:Analysis of Variance; D002423:Cause of Death; D002525:Cerebellar Cortex / Q000378:metabolism; D002531:Cerebellum / Q000378:metabolism*; D002675:Child, Preschool; D005260:Female; D016244:Guanosine 5'-O-(3-Thiotriphosphate) / Q000494:pharmacology; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D007457:Iodine Radioisotopes; D008297:Male; D011869:Radioligand Assay; D011956:Receptors, Cell Surface / Q000378:metabolism*; D044094:Receptors, Melatonin; D013398:Sudden Infant Death; D013577:Syndrome
D007457:Iodine Radioisotopes; D011956:Receptors, Cell Surface; D044094:Receptors, Melatonin; D016244:Guanosine 5'-O-(3-Thiotriphosphate)