Characterization of the Fad12 mutant of Synechocystis that is defective in delta 12 acyl-lipid desaturase activity.
The Fad12 mutant of Synechocystis sp. PCC 6803 has a defect in the desA gene for delta 12 acyl-lipid desaturase. We identified a change in the nucleotide sequence of the structural gene for the desaturase, in which a leucine codon has been converted to a stop codon. Western blot analysis revealed that the delta 12 acyl-lipid desaturase was localized in both plasma membranes and thylakoid membranes of wild-type cells but was absent from both types of membrane in Fad12 cells. These findings suggest that the desaturation of fatty acids takes place in both types of membrane in Synechocystis sp. PCC 6803. The mutation in the delta 12 desaturase did not affect the lipid composition of thylakoid and plasma membranes, but it changed the fatty acid composition of lipids in similar ways in both types of membrane.
Gombos
Z
Z
Department of Regulation Biology, National Institute for Basic Biology, Okazaki, Japan.
Wada
H
H
Varkonyi
Z
Z
Los
D A
DA
Murata
N
N
D000595:Amino Acid Sequence; D001483:Base Sequence; D001665:Binding Sites; D002462:Cell Membrane / Q000378:metabolism; D000458:Cyanobacteria / Q000235:genetics*; D044943:Fatty Acid Desaturases / Q000235:genetics* / Q000378:metabolism; D005227:Fatty Acids / Q000032:analysis; D007425:Intracellular Membranes / Q000378:metabolism; D008055:Lipids / Q000032:analysis; D008969:Molecular Sequence Data; D009154:Mutation; D010455:Peptides / Q000032:analysis
D005227:Fatty Acids; D008055:Lipids; D010455:Peptides; D044943:Fatty Acid Desaturases; C040448:delta-12 fatty acid desaturase