title: Cochlear implant-related osteoneogenesis in an animal model: fluorescent labeling.
abstract: Cochlear osteoneogenesis may result from a variety of pathologic conditions, including cochlear implantation. The etiology of cochlear osteoneogenesis following implantation, however, is not known. Cochlear implant-related osteoneogenesis has been demonstrated in laboratory animals, but the specific cause, extent, or time-course of this process has not been determined. In this preliminary study, fluorescent bone labels were used to assess osteoneogenesis in six chinchillas in three experimental categories: surgical trauma to the cochlea, chronic nonstimulated cochlear implantation, and intrascalar neomycin infusion. Computer image analysis was used to measure the area of labeled bone on representative mid-modiolar histologic sections. The amount of bone deposition was greatest in ears treated with intracochlear neomycin (mean = 2.3835 mm2, SD = 3.7308). Surgical trauma alone (mean = 0.9549 mm2, SD = 1.384) and chronic implantation without stimulation did not produce substantial bone growth when compared to contralateral control ears (mean = 0.0574 mm2, SD = 0.0731). Fluorochrome labeling was also used to differentiate types of bone deposition. The morphology and timing of new bone growth appeared to be related to the type of cochlear injury. These results confirm that intracochlear neomycin may contribute to osteoneogenesis in animal studies of cochlear implantation. This study supports the use of fluorescent bone labeling in the evaluation of cochlear osteoneogenesis.
authors.affiliation: Division of Otolaryngology, Head and Neck Surgery, Duke University Medical Center, Durham, North Carolina USA.
authors.forename: J E
authors.initials: JE
authors.lastname: Saunders
authors.forename: D W
authors.initials: DW
authors.lastname: Molter
authors.forename: J T
authors.initials: JT
authors.lastname: McElveen
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000009:adverse effects; D002114:Calcinosis / Q000209:etiology* / Q000473:pathology*; D002682:Chinchilla; D003051:Cochlea / Q000293:injuries; D015834:Cochlear Diseases / Q000209:etiology* / Q000473:pathology*; D003054:Cochlear Implants / Q000009:adverse effects*; D004195:Disease Models, Animal; D005456:Fluorescent Dyes; D006801:Humans; D007091:Image Processing, Computer-Assisted; D009355:Neomycin / Q000009:adverse effects; D010784:Photomicrography
chemical_list: D000900:Anti-Bacterial Agents; D005456:Fluorescent Dyes; D009355:Neomycin