title: Prognostic value of p53 protein accumulation in cancer cell nuclei in adenocarcinoma of the uterine cervix.
abstract: Adenocarcinoma of the uterine cervix (CxAd) is one of the most distressing malignancies of the female reproductive system because of its tendency to spread aggressively and to be resistant to radiation and systemic therapies. To clarify the prognostic significance of p53 alteration in CxAd, we immunohistochemically examined the incidence of p53 nuclear accumulation, which is considered to be mostly parallel with p53 gene mutation, and its association with clinicopathological parameters in 26 patients with CxAd. The overall incidence of p53 nuclear accumulation was 46% (12 of 26), being higher in groups with clinically advanced disease, higher degrees of cellular atypia, and deeper myometrial invasion, but significantly lower in patients with integration of human papillomavirus (HPV) type 16 or 18 DNA. Nuclear p53 immunoreactivity as well as lymph node status, depth of invasion and the absence of HPV-DNA integration were significant indicators of a poor prognosis. Examination of p53 nuclear accumulation could be applied to biopsy material, and would be of practical assistance in predicting the prognosis of CxAd both preoperatively and postoperatively.
authors.affiliation: Pathology Division, National Cancer Center Research Institute and Hospital, Tokyo.
authors.forename: H
authors.initials: H
authors.lastname: Tsuda
authors.forename: K
authors.initials: K
authors.lastname: Jiko
authors.forename: S
authors.initials: S
authors.lastname: Tsugane
authors.forename: M
authors.initials: M
authors.lastname: Yajima
authors.forename: T
authors.initials: T
authors.lastname: Yamada
authors.forename: K
authors.initials: K
authors.lastname: Tanemura
authors.forename: R
authors.initials: R
authors.lastname: Tsunematsu
authors.forename: K
authors.initials: K
authors.lastname: Ohmi
authors.forename: T
authors.initials: T
authors.lastname: Sonoda
authors.forename: S
authors.initials: S
authors.lastname: Hirohashi
mesh_terms: D000230:Adenocarcinoma / Q000737:chemistry* / Q000401:mortality / Q000473:pathology / Q000821:virology; D014408:Biomarkers, Tumor / Q000032:analysis*; D002467:Cell Nucleus / Q000737:chemistry*; D015897:Comorbidity; D015344:DNA Probes, HPV; D004273:DNA, Neoplasm / Q000032:analysis; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D016158:Genes, p53; D006801:Humans; D009362:Neoplasm Metastasis; D009363:Neoplasm Proteins / Q000032:analysis*; D009367:Neoplasm Staging; D027383:Papillomaviridae / Q000302:isolation & purification; D030361:Papillomavirus Infections / Q000453:epidemiology; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D011379:Prognosis; D015996:Survival Rate; D016159:Tumor Suppressor Protein p53 / Q000032:analysis*; D014412:Tumor Virus Infections / Q000453:epidemiology; D002583:Uterine Cervical Neoplasms / Q000737:chemistry* / Q000401:mortality / Q000473:pathology / Q000821:virology
chemical_list: D014408:Biomarkers, Tumor; D015344:DNA Probes, HPV; D004273:DNA, Neoplasm; D004279:DNA, Viral; D009363:Neoplasm Proteins; D016159:Tumor Suppressor Protein p53