title: Tissue pH in cold-stored human donor livers preserved in University of Wisconsin solution. A noninavasive clinical study with 31P-magnetic resonance spectroscopy.
abstract: It is not known whether the tissue acidosis that accompanies cold storage is the beginning of irreversible cell injury, ultimately leading to cell death, or whether it is a natural "protective" mechanism for cells to survive hypoxic periods. To answer this question, the tissue pH of 45 cold-stored human donor livers preserved in University of Wisconsin solution (UW) was assessed shortly before implantation using noninvasive 31P magnetic resonance spectroscopy. We conclude that tissue pH during cold storage may be partly dependent upon hepatic glycogen stores and donor age. The wide range of tissue pH values that was observed at the time of implantation does not result in significant effects on cellular damage after transplantation. This indicates that tissue pH is not a major determinant for the viability of UW solution-preserved human donor livers, as indicated by postoperative hepatocellular damage and liver synthesis function. The membrane stabilizing and buffering capacity of UW solution appears to protect liver viability against tissue acidosis. Our results also indicate that liver tissue pH can be lower than has been previously assumed in the literature without significant adverse effects on liver viability.
authors.affiliation: Department of Surgery, University Hospital, Groningen, The Netherlands.
authors.forename: R F
authors.initials: RF
authors.lastname: Wolf
authors.forename: J A
authors.initials: JA
authors.lastname: van der Hoeven
authors.forename: R L
authors.initials: RL
authors.lastname: Kamman
authors.forename: A L
authors.initials: AL
authors.lastname: Busza
authors.forename: R J
authors.initials: RJ
authors.lastname: Ploeg
authors.forename: W J
authors.initials: WJ
authors.lastname: Sluiter
authors.forename: M J
authors.initials: MJ
authors.lastname: Slooff
mesh_terms: D000241:Adenosine; D000293:Adolescent; D000328:Adult; D000493:Allopurinol; D002648:Child; D002675:Child, Preschool; D015925:Cryopreservation; D005978:Glutathione; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007223:Infant; D007328:Insulin; D008099:Liver; D016031:Liver Transplantation; D009682:Magnetic Resonance Spectroscopy; D008875:Middle Aged; D019188:Organ Preservation Solutions; D011887:Raffinose
chemical_list: D007328:Insulin; D019188:Organ Preservation Solutions; C055206:University of Wisconsin-lactobionate solution; D000493:Allopurinol; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose