8-Methoxypsoralen photoadduct formation in complementary oligonucleotides containing a cross-linkable site.
The complete profile of 8-methoxypsoralen photoadduct formation in complementary oligonucleotides (5'-GAGTATGAG and 5'-CATAC) has been determined. Equimolar solutions of the oligonucleotides were irradiated at 4 degrees C in order to stabilize the mini-double helix. Photomodified oligonucleotides were separated by reversed phase chromatography on a Vydac C4 column. Photoadduct formation favored the 5'TAT site in the 9mer over the 5'ATA site in the 5mer by a factor of two. Split-dose studies showed that the monoadducts formed on GAGTATGAG were preferentially converted to cross-links by an additional UVA exposure.
Park
J
J
Department of Dermatology, Yale University, New Haven, Connecticut, USA.
Amici
L
L
Gasparro
F P
FP
D000225:Adenine / Q000032:analysis / Q000528:radiation effects; D001482:Base Composition; D002851:Chromatography, High Pressure Liquid; D003432:Cross-Linking Reagents / Q000494:pharmacology*; D003596:Cytosine / Q000032:analysis / Q000528:radiation effects; D018736:DNA Adducts / Q000032:analysis / Q000187:drug effects* / Q000528:radiation effects; D006147:Guanine / Q000032:analysis / Q000528:radiation effects; D008027:Light; D008730:Methoxsalen / Q000494:pharmacology*; D009841:Oligonucleotides / Q000032:analysis / Q000528:radiation effects; D017319:Photosensitizing Agents / Q000494:pharmacology*; D013941:Thymine / Q000032:analysis / Q000528:radiation effects; D014316:Tritium; D014466:Ultraviolet Rays
D003432:Cross-Linking Reagents; D018736:DNA Adducts; D009841:Oligonucleotides; D017319:Photosensitizing Agents; D014316:Tritium; D006147:Guanine; D003596:Cytosine; D000225:Adenine; D013941:Thymine; D008730:Methoxsalen