Complete nucleotide sequence, structural organization, and an alternatively spliced exon of mouse h1-calponin gene.
From a CC1.2 embryonic stem cell genomic library, we isolated and sequenced a 10.4-kb DNA segment (GenBank/EMBL Data Bank accession number L49022) containing the entire gene encoding mouse h1-calponin, an actin-associated smooth muscle-specific protein and a potential modulator of contraction. Sequence data revealed that there are seven exons and six introns in the h1-calponin gene. Determined by primer extension mapping of the RNA transcripts, the transcription of h1-calponin gene initiates at the same site in stomach, urinary bladder and pregnant uterus smooth muscles. The genomic organization suggests that the previously identified alpha- and beta-calponin isoforms are produced by splicing of exon 7 at two alternative acceptor sites. Isolation and structural characterization of the h1-calponin gene provides information to further investigate the expression regulation of this smooth muscle-specific gene.
Gao
J
J
Department of Medical Biochemistry, University of Calgary Faculty of Medicine, Alberta, Canada.
Hwang
J M
JM
Jin
J P
JP
D017398:Alternative Splicing; D000818:Animals; D001483:Base Sequence; D002135:Calcium-Binding Proteins / Q000096:biosynthesis / Q000235:genetics*; D003001:Cloning, Molecular; D017931:DNA Primers; D005091:Exons; D005260:Female; D005753:Gastric Mucosa / Q000378:metabolism; D015698:Genomic Library; D051379:Mice; D008840:Microfilament Proteins; D008969:Molecular Sequence Data; D009124:Muscle Proteins / Q000096:biosynthesis / Q000235:genetics; D009130:Muscle, Smooth / Q000378:metabolism*; D011247:Pregnancy; D015183:Restriction Mapping; D013234:Stem Cells / Q000378:metabolism*; D001743:Urinary Bladder / Q000378:metabolism; D014599:Uterus / Q000378:metabolism; D000096985:Calponins
D002135:Calcium-Binding Proteins; D017931:DNA Primers; D008840:Microfilament Proteins; D009124:Muscle Proteins; D000096985:Calponins