Characterization of the hog cholera virus 5' terminus.
Hog cholera virus (HoCV) 5' terminus of the ALD and GPE(-) strains were analyzed by using rapid amplification of cDNA end method (5'RACE). An additional nine nucleotides were found at the 5' termini of genomic RNA in the ALD and GPE(-) strains of HoCV. These nine nucleotides were also conserved in BVDV and were suggested to form a hairpin structure at the 5' terminus by computer-assisted analysis. It seems possible that the secondary structure and/or the 5' terminus sequence has a significant role in the HoCV virus genome.
Katayama
K
K
Basic Research Division, BioMedical Laboratories, Saitama, Japan.
Kurihara
C
C
Fukushi
S
S
Hoshino
F B
FB
Ishikawa
K
K
Nagai
H
H
Ando
T
T
Oya
A
A
D000818:Animals; D001483:Base Sequence; D006692:Classical Swine Fever Virus / Q000235:genetics*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012367:RNA, Viral / Q000737:chemistry*; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D014613:Vaccines, Attenuated / Q000235:genetics; D014765:Viral Vaccines / Q000235:genetics
D012367:RNA, Viral; D014613:Vaccines, Attenuated; D014765:Viral Vaccines