HCC-1, a novel chemokine from human plasma.
A novel CC chemokine, HCC-1, was isolated from the hemofiltrate of patients with chronic renal failure. HCC-1 has a relative molecular mass of 8,673 and consists of 74 amino acids including four cysteines linked to disulfide bonds. HCC-1 cDNA was cloned from human bone marrow and shown to code for the mature protein plus a putative 19-residue leader sequence. Mature HCC-1 has sequence identity of 46% with macrophage inflammatory protein (MIP)-1 alpha and MIP-1 beta, and 29-37% with the other human CC chemokines. Unlike MIP-1 alpha and the other CC chemokines, HCC-1 is expressed constitutively in several normal tissues (spleen, liver, skeletal and heart muscle, gut, and bone marrow), and is present at high concentrations (1-80 nM) in plasma. HCC-1 has weak activities on human monocytes and acts via receptors that also recognize MIP-1 alpha. It induced intracellular Ca2+ changes and enzyme release, but no chemotaxis, at concentrations of 100-1,000 nM, and was inactive on T lymphocytes, neutrophils, and eosinophil leukocytes. In addition, HCC-1 enhanced the proliferation of CD34+ myeloid progenitor cells. It was as effective as MIP-1 alpha, but about 100-fold less potent.
Schulz-Knappe
P
P
Lower Saxony Institute for Peptide Research (IPF), Hannover, Germany.
Mägert
H J
HJ
Dewald
B
B
Meyer
M
M
Cetin
Y
Y
Kubbies
M
M
Tomeczkowski
J
J
Kirchhoff
K
K
Raida
M
M
Adermann
K
K
Kist
A
A
Reinecke
M
M
Sillard
R
R
Pardigol
A
A
Uguccioni
M
M
Baggiolini
M
M
Forssmann
W G
WG
D000595:Amino Acid Sequence; D001483:Base Sequence; D002118:Calcium / Q000378:metabolism; D054407:Chemokine CCL4; D018925:Chemokines / Q000737:chemistry / Q000235:genetics* / Q000494:pharmacology; D019742:Chemokines, CC; D003001:Cloning, Molecular; D016207:Cytokines / Q000494:pharmacology; D018076:DNA, Complementary / Q000235:genetics; D006801:Humans; D007676:Kidney Failure, Chronic / Q000097:blood*; D019402:Macrophage Inflammatory Proteins; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009000:Monocytes / Q000187:drug effects; D015846:Monokines / Q000235:genetics / Q000494:pharmacology; D017421:Sequence Analysis; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution
C097720:CCL14 protein, human; D054407:Chemokine CCL4; D018925:Chemokines; D019742:Chemokines, CC; D016207:Cytokines; D018076:DNA, Complementary; D019402:Macrophage Inflammatory Proteins; D015846:Monokines; D002118:Calcium