title: Processing and presentation of type II collagen, a fibrillar autoantigen, by H-2q antigen-presenting cells.
abstract: Native type II collagen (CII) is a high molecular-weight fibrillar molecule which induces a chronic polyarthritis in mice expressing the H-2q haplotype. The present study was initiated to analyze the processing and the presentation of this nonglobular protein by H-2q antigen-presenting cells (APC). Efficiency of presentation was assessed by the ability of antigen-pulsed APC to activate collagen-specific CD4+ T cell hybridomas. Fixation of APC or the presence of chloroquine completely blocked the reactivity of the T cell hybrids to native, denatured and cyanogen bromide (CB) degraded CII, thus indicating the requirement of intracellular processing for adequate presentation of CII peptides to T cells. In the presence of various processing inhibitors (brefeldin A, leupeptin and N-tosyl-L-phenylalanine chloromethylketone) stimulation of T hybrids by CII-pulsed APC was reduced, pointing to the need of newly synthesized class II molecules, the use of several intracellular compartments and the implications of different proteases in the generation of CII peptides. Peritoneal macrophages and, to a lesser extent, total spleen cells, presented native and denatured CII with higher efficiency than purified splenic dendritic cells, naive or even immune B cells from CII-primed mice. In contrast, these dendritic and B cells were fully competent to present intact ovalbumin to a specific T cell hybrid. The stimulation by dendritic cells and immune B cells was greater when CB peptides of CII were added instead of the native molecule. Similarly, the cleavage of CII was an absolute requirement for its presentation by epidermal cells and B cell lymphomas to the T cell hybridomas. Taken together, these findings emphasize the crucial role of intracellular processing for recognition of soluble CII, similar to most antigens. However, in contrast to ovalbumin, the size and fibrillar nature of the native CII molecule influences its capture by the APC, thus limiting the type of APC able to present this antigen.
authors.affiliation: INSERM U 283, Hôpital Cochin, Université René Descartes, Paris, France.
authors.forename: B
authors.initials: B
authors.lastname: Manoury-Schwartz
authors.forename: G
authors.initials: G
authors.lastname: Chiocchia
authors.forename: C
authors.initials: C
authors.lastname: Fournier
mesh_terms: D000818:Animals; D017951:Antigen Presentation; D000938:Antigen-Presenting Cells / Q000502:physiology; D001324:Autoantigens / Q000276:immunology*; D002417:Cattle; D003094:Collagen / Q000037:antagonists & inhibitors / Q000276:immunology* / Q000378:metabolism*; D051379:Mice; D008811:Mice, Inbred DBA
chemical_list: D001324:Autoantigens; D003094:Collagen