Evaluation of DNA fingerprinting by PFGE as an epidemiologic tool for Salmonella infections.
To evaluate DNA fingerprinting as an epidemiologic tool, pulsed-field gel electrophoresis (PFGE) was performed on isolates of Salmonella, including S. typhimurium, S. thompson, and S. enteritidis. Chromosomal DNA was digested with the restriction endonucleases Bln I and Xba I. The patterns of S. thompson and S. typhimurium isolates from various sources were different from one another. There was no correlation between the phage type and the digestion pattern of S. enteritidis isolates. Some strains belonging to one phage type were distinguished by their PFGE pattern in this study. These results suggest that the Bln I and Xba I digestion patterns of chromosomal DNA are useful for epidemiological analysis of an outbreak of Salmonella infection or food poisoning.
Murase
T
T
Department of Bacteriology and Pathology, Kanagawa Prefectural Public Health Laboratory, Japan.
Okitsu
T
T
Suzuki
R
R
Morozumi
H
H
Matsushima
A
A
Nakamura
A
A
Yamai
S
S
D016172:DNA Fingerprinting; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000032:analysis*; D015252:Deoxyribonucleases, Type II Site-Specific; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D005069:Evaluation Studies as Topic; D006801:Humans; D017720:Molecular Epidemiology; D010641:Phenotype; D012475:Salmonella / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D012478:Salmonella Food Poisoning / Q000453:epidemiology / Q000382:microbiology; D012480:Salmonella Infections / Q000453:epidemiology* / Q000382:microbiology
D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes; C025580:endodeoxyribonuclease XBAI; C064053:CCTAGG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific