[Studies on the enzyme cytochemistry of Toxoplasma gondii and the influence of artemether].
Male mice of Kunming strain were infected with 10,000 tachyzoites intraperitoneally. 2 hours after infection the mice were divided into 2 groups and administered 5% of amylum and 200 mg/kg of artemether by gavage for 8 consecutive days. The ultrastructural enzyme cytochemical studies on cytidine monophosphatase (CMP-ase) and glucose-6-phosphatase (G-6-P-ase) of the parasites were carried out. CMP-ase was found scattered in the lysosomes of the parasites as well as in the macrophages. No differences were observed in the localization and intensity of CMP-ase activity between the nontreated and treated with drug parasites. G-6-P-ase was found surrounding the parasite membrane and scattered in the parasitophorous vacuole in the nontreated parasites. After treatment with artemether, the intensity of G-6-P-ase activity was decreased compared with nontreated control parasites. It is suggested that artemether may exert some action on the G-6-P-ase of T. gondii and thus influence the energy metabolis of the parasite.
Liang
Z
Z
Department of Parasitology, School of Basic Medical Sciences, Shanghai Medical University.
Xu
L
L
D000818:Animals; D000077549:Artemether; D037621:Artemisinins; D003049:Coccidiostats / Q000494:pharmacology*; D005952:Glucose-6-Phosphatase / Q000378:metabolism*; D006651:Histocytochemistry; D008297:Male; D051379:Mice; D008854:Microscopy, Electron; D009708:Nucleotidases / Q000378:metabolism*; D012717:Sesquiterpenes / Q000494:pharmacology*; D014122:Toxoplasma / Q000201:enzymology* / Q000648:ultrastructure
D037621:Artemisinins; D003049:Coccidiostats; D012717:Sesquiterpenes; D000077549:Artemether; D009708:Nucleotidases; C025221:cytidine-5'-monophosphatase; D005952:Glucose-6-Phosphatase