[Chronological analysis of eosinophil infiltration in mite-allergen induced dermatitis in atopic patients].
To elucidate the active process of eosinophil infiltration in atopic dermatitis (AD), we performed patch testing using crude mite allergens on normal-appearing skin in adult AD patients with high levels of mite-specific IgE antibodies and analyzed positive reaction sites chronologically by histological and immunohistological methods. Positive reactions were observed in 6 out of 9 AD patients (66.7%), whereas none of 7 non-AD control subjects showed positive reactions. Skin biopsies were obtained from positive reaction sites at 2,6,12,24 and 48 hours after allergen challenge. Eosinophils were seen within postcapillary venules at 2 hours, followed by increased perivascular eosinophil infiltration at 6 hours which peaked at 24 and 48 hours. Epidermal eosinophilic spongiosis was also observed at 48 hours. The majority of infiltrating eosinophils showed positive reactions for BMK-13 and 15-59% of them were EG2 positive activated eosinophils. BMK-13, EG2 and anti-EP positive reactions were scattered throughout connective tissue and their areas were time-dependently increased. These findings suggest that eosinophil transmigration and activation play an important role in initiating early AD lesions induced by transepidermal mite allergen permeation.
Wakugawa
M
M
Department of Dermatology, Faculty of Medicine, University of Tokyo.
Yamada
N
N
Nakagawa
H
H
Tamaki
K
K
D000328:Adult; D000485:Allergens / Q000276:immunology*; D000818:Animals; D001798:Blood Proteins / Q000378:metabolism; D002465:Cell Movement; D003876:Dermatitis, Atopic / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D004802:Eosinophilia / Q000473:pathology*; D004804:Eosinophils / Q000276:immunology*; D005260:Female; D006801:Humans; D008297:Male; D008925:Mites / Q000276:immunology*; D010328:Patch Tests
D000485:Allergens; D001798:Blood Proteins