Oral inoculation of SCID mice with an attenuated herpes simplex virus-1 strain causes persistent enteric nervous system infection and gastric ulcers without direct mucosal infection.
BACKGROUND
After placement of herpes simplex virus type 1 (HSV-1) into the esophageal lumen of BALB/c mice, the virus replicated in enteric neurons within the esophagus and stomach and was transported to the sensory ganglia of the vagus nerve (nodose ganglia), where viral replication also occurs and where ultimately a long term latent infection is established. This described infection of immunocompetent mice primarily involved neuronal cells and associated satellite cells.


METHODS
Severe combined immunodeficient (SCID) mice were orally infected with an attenuated strain of HSV-1 to better identify sites of viral involvement in the gastrointestinal tract, particularly the mucosa.


RESULTS
Three to five weeks after oral inoculation of SCID mice with HSV-1 strain in1814, a persistent viral infection of the gastrointestinal tract was established in most of the mice. Extensive viral replication was detected by immunohistochemistry throughout pathways of the vagus nerve and within the intrinsic enteric nervous system. Despite this ultimately fatal infection, viral replication in the gut occurred almost exclusively in enteric neurons and their processes; viral proteins were occasionally seen in smooth muscle cells immediately adjacent to heavily infected enteric ganglia. More than 50% of these persistently infected mice, when killed 18 to 31 days postinoculation, had gastric ulcers that were identified grossly and histologically. Only one of the 40 gastric ulcers was found to contain viral Ag. The remaining ulcers, although devoid of viral proteins, were found adjacent to virus-infected ganglia.


CONCLUSIONS
HSV-1 can enter enteric neurons with minimal initial mucosal involvement, and once inside the nervous system, the virus is contained there despite the absence of a specific host immune response. Furthermore, chronically infected enteric neurons may provide an indirect mechanism for the pathogenesis of gastric ulcers in these immune-deficient mice.
Gesser
R M
RM
Division of Allergy, Immunology and Infectious Diseases, Children's Hospital of Philadelphia, Pennsylvania, USA.
Valyi-Nagy
T
T
Fraser
N W
NW
Altschuler
S M
SM
D000284:Administration, Oral; D000818:Animals; D001933:Brain Stem / Q000382:microbiology / Q000473:pathology; D004064:Digestive System / Q000294:innervation / Q000473:pathology / Q000821:virology*; D017615:Enteric Nervous System / Q000473:pathology / Q000821:virology*; D005260:Female; D005753:Gastric Mucosa / Q000821:virology*; D006561:Herpes Simplex / Q000150:complications* / Q000473:pathology; D018259:Herpesvirus 1, Human / Q000472:pathogenicity*; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D051379:Mice; D008807:Mice, Inbred BALB C; D016513:Mice, SCID; D009620:Nodose Ganglion / Q000473:pathology / Q000821:virology; D012367:RNA, Viral / Q000032:analysis; D013276:Stomach Ulcer / Q000209:etiology*
D012367:RNA, Viral