title: Foreign anion substitution for chloride in neonatal human red cells. Effect on cellular volume.
abstract: The effect of replacement of chloride by thiocyanate has been investigated in neonatal human red cells. On incubating these cells in isotonic SCN- medium, a rapid cellular swelling was observed, which was not evident in Cl- media. Incubation in hypertonic SCN- medium showed a rapid osmotic shrinkage followed by reswelling back to the initial volume. This regulatory volume increase was completed in a shorter time than in Cl- medium. Whole water accumulated by the cells in both experimental conditions can be accounted for by net Na+ uptake (twofold augmentation with respect to initial value). Amiloride inhibits specifically both cellular swelling and the Na+ content increase in these experimental conditions. Similar experiments in neonatal red cells incubated in NO3- media, as well as in adult red cells placed in NO3- or SCN- media, did not exhibit the same response. The findings suggest that the response of neonatal red cells in a SCN- containing media was mediated by activation of a Na+/H+ antiport mechanism.
authors.affiliation: Catedra de Fisiologia, Fac. Ciencias Medicas UNR, Santa Fe, Rosario, Argentina.
authors.forename: R E
authors.initials: RE
authors.lastname: Serrani
authors.forename: D
authors.initials: D
authors.lastname: Taborda
authors.forename: M
authors.initials: M
authors.lastname: Zamero
authors.forename: J L
authors.initials: JL
authors.lastname: Corch
mesh_terms: D000328:Adult; D000584:Amiloride / Q000494:pharmacology; D000838:Anions; D002712:Chlorides / Q000097:blood*; D004912:Erythrocytes / Q000166:cytology* / Q000187:drug effects; D006801:Humans; D006982:Hypertonic Solutions; D007038:Hypotonic Solutions; D007231:Infant, Newborn; D012964:Sodium / Q000097:blood; D017923:Sodium-Hydrogen Exchangers / Q000097:blood; D013861:Thiocyanates / Q000097:blood*
chemical_list: D000838:Anions; D002712:Chlorides; D006982:Hypertonic Solutions; D007038:Hypotonic Solutions; D017923:Sodium-Hydrogen Exchangers; D013861:Thiocyanates; D000584:Amiloride; D012964:Sodium; C031760:thiocyanate