title: Induction, not associated with host-cell re-activation of damaged plasmid DNA, of damaged-DNA-recognition proteins by retinoic acid and dibutyryl cyclic AMP in mammalian cells.
abstract: Our previous studies [Chao (1992) Biochem. J. 282, 203-207; C.C.-K. Chao, unpublished work] has suggested a correlation between the levels of constitutive UV-damaged-DNA-recognitionproteins (UVDRP) and cellular DNA repair in different cell types. In the present study, UVDRP were induced in F9 and NIH3T3 cells by 0.1 microM retinoic acid (RA) and 1 mM dibutyryl cyclic AMP (dbcAMP), which is sufficient to induce differentiation in murine F9 stem cells. The induction of UVDRP in F9 and NIH3T3 cells was optimized after 6 and 2 days incubation with RA/dbcAMP respectively. Since NIH3T3 cells were not induced to differentiate by RA/dbcAMP, the upregulation of the UVDRP in mammalian cells would thus seem not to be mediated directly by differentiation. Using a plasmid re-activation assay to estimate DNA repair, we did not find a correlation between DNA repair and UVDRP in RA/dbcAMP-treated cells. The results suggest that UVDRP may have a function other than, or in addition to, its role in DNA repair.
authors.affiliation: Department of Biochemistry, Chang Gung Medical College, Taoyuan, Taiwan, Republic of China.
authors.forename: N K
authors.initials: NK
authors.lastname: Sun
authors.forename: S L
authors.initials: SL
authors.lastname: Huang
authors.forename: S
authors.initials: S
authors.lastname: Lin-Chao
authors.forename: C C
authors.initials: CC
authors.lastname: Chao
mesh_terms: D000818:Animals; D003994:Bucladesine / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects; D002460:Cell Line; D004260:DNA Repair; D004274:DNA, Recombinant / Q000096:biosynthesis*; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000378:metabolism*; D006801:Humans; D051379:Mice; D010957:Plasmids; D014212:Tretinoin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured; D014466:Ultraviolet Rays
chemical_list: D004274:DNA, Recombinant; D004268:DNA-Binding Proteins; D014212:Tretinoin; D003994:Bucladesine