Improvement of Salmonella detection on motility enrichment media by ferrioxamine E-supplementation of pre-enrichment culture.
Supplementation of buffered peptone water with ferrioxamine E in concentrations ranging from 0.001 to 1.0 microgram/ml significantly increased the motility of Salmonella on MSRV and DIASSALM semi-solid enrichment media. Zone diameters of swarming S. enteritidis and S. typhimurium increased more than twofold following use of pre-enrichment cultures supplemented with 0.01 microgram ferrioxamine E/ml. The activity of ferrioxamine E is similar at 37 degrees C and 42 degrees C. Pre-enrichment of Salmonella in a variety of foods in supplemented buffered peptone water, with shaking at 37 degrees C for 6 h and motility enrichment at 42 degrees C for 16 h, enabled motile Salmonella to be detected in 1 day.
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Fachabteilung für das Veterinärwesen beim Amt der Steiermärkischen Landesregierung, Graz, Austria.
Reissbrodt
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D003470:Culture Media / Q000737:chemistry*; D005290:Ferric Compounds; D008828:Microbiological Techniques; D010456:Peptides, Cyclic; D010461:Peptones; D012475:Salmonella / Q000187:drug effects / Q000302:isolation & purification* / Q000502:physiology; D013696:Temperature
D003470:Culture Media; D005290:Ferric Compounds; D010456:Peptides, Cyclic; D010461:Peptones; C017508:ferrioxamine E