Lysophosphatidylcholine inhibits NMDA-induced currents by a mechanism independent of phospholipase A2-mediated protein kinase C activation in hippocampal glial cells.
Lysophosphatidylcholine (LysoPC), which is formed by phospholipase A2 (PLA2)-mediated phosphatidylcholine hydrolysis, is involved in enhancement of the diacylglycerol- or phorbol ester-dependent protein kinase C (PKC) activation. In the present study, the effect of lysoPC on NMDA-evoked currents was examined by whole-cell patches in cultured rat hippocampal glial cells. NMDA activated the receptor channel and produced currents in glial cells. LysoPC reversibly inhibited the NMDA-gated currents by 43% and this inhibition was not affected by a selective PKC inhibitor, GF109203X. These results indicate that lysoPC inhibits NMDA-induced currents by a mechanism independent of PLA2-mediated PKC activation. A possible explanation for the effect could be that lysoPC directly alters channel-gating kinetics of NMDA receptor or interacts with membrane components surrounding NMDA receptors.
Ikeuchi
Y
Y
Department of Physiology, Kobe University School of Medicine, Japan.
Nishizaki
T
T
Matsuoka
T
T
D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D004594:Electrophysiology; D004789:Enzyme Activation / Q000187:drug effects; D006624:Hippocampus / Q000166:cytology; D015640:Ion Channel Gating; D008244:Lysophosphatidylcholines / Q000494:pharmacology*; D016202:N-Methylaspartate / Q000037:antagonists & inhibitors*; D009457:Neuroglia / Q000502:physiology*; D010741:Phospholipases A / Q000502:physiology*; D054467:Phospholipases A2; D011493:Protein Kinase C / Q000502:physiology*; D051381:Rats; D016194:Receptors, N-Methyl-D-Aspartate / Q000502:physiology*
D008244:Lysophosphatidylcholines; D016194:Receptors, N-Methyl-D-Aspartate; D016202:N-Methylaspartate; D011493:Protein Kinase C; D010741:Phospholipases A; D054467:Phospholipases A2