PCR-based identification of wheat genomes.
We describe a PCR system that distinguishes the A, B and D genomes in wheat DNA extracts. PCRs were directed at the 'non-transcribed spacer' regions of the rDNA loci. The spacers within the D genome locus have a 71-bp insertion that is absent from the corresponding A and B loci. PCR product sizes therefore enable D- and D+ genomes to be distinguished. The A and B genomes can be differentiated by PCR with an internal primer which does not anneal to A genome sequences. This work is relevant to the ancient ecology of wheat, as it is often difficult to determine ploidy level from morphological examination of archaeobotanical remains.
Sallares
R
R
Department of Biochemistry and Applied Molecular Biology, UMIST, Manchester, UK.
Allaby
R G
RG
Brown
T A
TA
D001483:Base Sequence; D015139:Blotting, Southern; D017931:DNA Primers; D018744:DNA, Plant / Q000235:genetics*; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005828:Genetics, Population; D018745:Genome, Plant; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011123:Polyploidy; D014158:Transcription, Genetic; D014908:Triticum / Q000235:genetics*
D017931:DNA Primers; D018744:DNA, Plant; D004275:DNA, Ribosomal