[Changes in the proteolytic activity of venous blood leukocyte nuclei from gamma-irradiated rabbits].
In terms of their capacity to decompose a casein substrate, the activity of nuclear proteinases was studied in the leukocytes of the rabbit blood taken before and after their joint gamma-irradiation. There was a substantial increase in the activity of proteinases in the leukocytic nuclei 2 and 48 hours after animals had been irradiated in a dose of 5 Gy as compared with those obtained prior to irradiation. Ninety six hours after irradiation the activity showed an average decrease to 40-60% of the control level. There was a correlation between the proteolytic activity and the irradiation dose: there were average 1.75- and 3.25-fold activity increases with 2.5 and 5.0 Gy, respectively. The findings provide a rationale for examining the proteinase activity of human peripheral leukocytic nuclei to clinically estimate radiation exposures.
Surkenova
G N
GN
Malakhova
L V
LV
Gaziev
A I
AI
D000818:Animals; D002467:Cell Nucleus / Q000201:enzymology / Q000378:metabolism / Q000528:radiation effects*; D010450:Endopeptidases / Q000378:metabolism; D006801:Humans; D006868:Hydrolysis; D007962:Leukocytes / Q000201:enzymology / Q000378:metabolism / Q000528:radiation effects*; D011817:Rabbits; D014680:Veins
D010450:Endopeptidases
Izmenenie proteoliticheskoĭ aktivnosti iader leĭkotsitov venoznoĭ krovi gamma-obluchennykh krolikov.