title: On-line coupling of micellar electrokinetic chromatography to electrospray mass spectrometry.
abstract: The possibility of selectivity enhancement in capillary electrophoresis-mass spectrometry (CE-MS) by hyphenating micellar electrokinetic chromatography (MEKC) and electrospray mass spectrometry (MS) is described for two quaternary ammonium compounds. Direct coupling of MEKC to MS is hazardous because of the contamination of the ion source due to presence of an excess of micelle forming agent in the MEKC buffer. Therefore, a coupled-capillary setup with the possibilities of voltage switching and buffer renewal has been designed. Such a system allows on-line heartcutting of the zones of interest in the MEKC capillary with subsequent transfer via a second capillary to the mass spectrometer.
authors.affiliation: Division of Analytical Chemistry, Leiden/Amsterdam Center for Drug Research, Leiden University, Netherlands.
authors.forename: M H
authors.initials: MH
authors.lastname: Lamoree
authors.forename: U R
authors.initials: UR
authors.lastname: Tjaden
authors.forename: J
authors.initials: J
authors.lastname: van der Greef
mesh_terms: D002021:Buffers; D002197:Capillary Action; D002845:Chromatography / Q000379:methods*; D004586:Electrophoresis; D007700:Kinetics; D013058:Mass Spectrometry / Q000379:methods*; D008823:Micelles
chemical_list: D002021:Buffers; D008823:Micelles