Optimal preservation of the J1-31 antigen in astroglia examined by immunogold labelling.
Several methods for the detection of immunogold labelling for the monoclonal antibody (Mab) J1-31 in astroglial cells are described. The optimal preservation of the gold labelling is achieved in sections after cryoultramicrotomy or LR white embedding. The results obtained are consistent with the previous findings that the Mab J1-31 antigen is an intermediate filament-associated protein in the astroglial cells.
Bhatnagar
R
R
Department of Biological Sciences, University of Alberta, Edmonton, Canada.
Malhotra
S K
SK
D000818:Animals; D000911:Antibodies, Monoclonal; D000941:Antigens / Q000032:analysis*; D001253:Astrocytes / Q000737:chemistry / Q000276:immunology*; D001254:Astrocytoma; D001921:Brain / Q000166:cytology; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods* / Q000592:standards; D051381:Rats; D013116:Spinal Cord / Q000166:cytology; D016610:Tissue Embedding; D014407:Tumor Cells, Cultured / Q000166:cytology
D000911:Antibodies, Monoclonal; D000941:Antigens; D005904:Glial Fibrillary Acidic Protein