Synthesis and properties of cross-linked DNA duplexes.
A method has been devised to synthesize DNA duplexes with covalently connected strands. The structure of cross-linked duplexes was confirmed by a reaction with the restriction endonuclease AluI. The thermal stability of the resulting compounds was investigated.
Antsypovich
S I
SI
Chemistry Department, Moscow State University, Russian Federation.
Oretskaya
T S
TS
Romanova
E A
EA
Volkov
E M
EM
Tashlitsky
V N
VN
Vasser
M
M
Shabarova
Z A
ZA
D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003432:Cross-Linking Reagents / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000737:chemistry / Q000378:metabolism; D004586:Electrophoresis / Q000379:methods; D006358:Hot Temperature; D008958:Models, Molecular; D008969:Molecular Sequence Data; D016328:NF-kappa B / Q000737:chemistry / Q000378:metabolism; D009841:Oligonucleotides / Q000138:chemical synthesis; D013057:Spectrum Analysis; D013696:Temperature; D014466:Ultraviolet Rays; D014529:Uridine / Q000031:analogs & derivatives / Q000737:chemistry
D003432:Cross-Linking Reagents; D016328:NF-kappa B; D009841:Oligonucleotides; D004247:DNA; C024669:endodeoxyribonuclease AluI; D015252:Deoxyribonucleases, Type II Site-Specific; D014529:Uridine