title: CWH41 encodes a novel endoplasmic reticulum membrane N-glycoprotein involved in beta 1,6-glucan assembly.
abstract: CWH41 encodes a novel type II integral membrane N-glycoprotein located in the endoplasmic reticulum. Disruption of the CWH41 gene leads to a K1 killer toxin-resistant phenotype and a 50% reduction in the cell wall beta 1,6-glucan level. CWH41 also displays strong genetic interactions with KRE1 and KRE6, two genes known to be involved in the beta 1,6-glucan biosynthetic pathway. The cwh41 delta kre6 delta double mutant is nonviable; and the cwh41 delta kre1 delta double mutation results in strong synergistic defects, with a severely slow-growth phenotype, a 75% reduction in beta 1,6-glucan level, and the secretion of a cell wall glucomannoprotein, Cwp1p. These results provide strong genetic evidence indicating that Cwh41p plays a functional role, possibly as a new synthetic component, in the assembly of cell wall beta 1,6-glucan.
authors.affiliation: Department of Biology, McGill University, Montreal, Quebec, Canada.
authors.forename: B
authors.initials: B
authors.lastname: Jiang
authors.forename: J
authors.initials: J
authors.lastname: Sheraton
authors.forename: A F
authors.initials: AF
authors.lastname: Ram
authors.forename: G J
authors.initials: GJ
authors.lastname: Dijkgraaf
authors.forename: F M
authors.initials: FM
authors.lastname: Klis
authors.forename: H
authors.initials: H
authors.lastname: Bussey
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D015153:Blotting, Western; D002451:Cell Compartmentation; D002473:Cell Wall / Q000378:metabolism*; D003001:Cloning, Molecular; D003433:Crosses, Genetic; D004352:Drug Resistance, Microbial; D004721:Endoplasmic Reticulum / Q000737:chemistry*; D005656:Fungal Proteins / Q000032:analysis / Q000235:genetics*; D005800:Genes, Fungal; D005936:Glucans / Q000378:metabolism*; D006023:Glycoproteins / Q000378:metabolism; D055646:Killer Factors, Yeast; D008562:Membrane Glycoproteins / Q000032:analysis / Q000235:genetics* / Q000276:immunology; D008565:Membrane Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D009154:Mutation; D009183:Mycotoxins / Q000494:pharmacology; D010641:Phenotype; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000187:drug effects / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D017422:Sequence Analysis, DNA; D000520:alpha-Glucosidases; D047071:beta-Glucans
chemical_list: D005656:Fungal Proteins; D005936:Glucans; D006023:Glycoproteins; C089359:K1 killer toxin; C064013:KRE1 protein, S cerevisiae; C081933:KRE6 protein, S cerevisiae; D055646:Killer Factors, Yeast; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D009183:Mycotoxins; D029701:Saccharomyces cerevisiae Proteins; D047071:beta-Glucans; C064197:beta-1,6-glucan; C098060:CWH41 protein, S cerevisiae; D000520:alpha-Glucosidases