Use of non-subjective analysis of lens transparency in experimental radiation cataract research.
Historically the major impediment to radiation cataract follow-up has been the necessarily subjective nature of assessing the degree of lens transparency. This has spurred the development of instruments which produce video images amenable to digital analysis. One such system, the Zeiss Scheimpflug slit lamp measuring system (SLC), was incorporated into our ongoing studies of radiation cataractogenesis. It was found that the Zeiss SLC measuring system has high resolution and permits the acquisition of reproducible images of the anterior segment of the eye. Our results, based on about 650 images of lenses followed over a period of 91 weeks of radiation cataract development, showed that the changes in the light scatter of the lens correlated well with conventional assessment of radiation cataracts with the added advantages of objectivity, permanent and transportable records and linearity as cataracts become more severe. This continuous data acquisition, commencing with cataract onset, can proceed through more advanced stages. The SLC exhibits much greater sensitivity reflected in a continuously progressive severity thereby avoiding the artifactual plateaus in staging which occur using conventional scoring methods.
Worgul
B V
BV
Columbia University, Department of Ophthalmology, New York, N.Y. 10032, USA.
Medvedovsky
C
C
Wu
B
B
D000818:Animals; D000869:Anterior Eye Segment / Q000473:pathology / Q000528:radiation effects; D002386:Cataract / Q000473:pathology / Q000503:physiopathology*; D005500:Follow-Up Studies; D007091:Image Processing, Computer-Assisted; D007908:Lens, Crystalline / Q000502:physiology* / Q000528:radiation effects; D010781:Photography / Q000379:methods; D011833:Radiation Injuries, Experimental / Q000473:pathology / Q000503:physiopathology*; D011839:Radiation, Ionizing; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results