title: Cloning and expression of a single-chain antibody fragment specific for a monomorphic determinant of class I molecules of the human major histocompatibility complex.
abstract: B9.12.1 is a monoclonal antibody specific for a monomorphic determinant of human MHC class I molecules. It is currently used for cell typing and is useful for targeting infection of human cells by murine ecotropic retroviruses. We have cloned and expressed it in the form of a single-chain variable fragment (ScFv) that recognizes the same epitope as the parental antibody. Through genetic engineering, this ScFv may be used for developing new cell-typing probes and new retroviral targeting approaches.
authors.affiliation: Institut de Génétique Moléculaire de Montpellier, UMR9942/CNRS, France.
authors.forename: M
authors.initials: M
authors.lastname: Marin
authors.forename: F
authors.initials: F
authors.lastname: Brockly
authors.forename: D
authors.initials: D
authors.lastname: Noel
authors.forename: M
authors.initials: M
authors.lastname: Etienne-Julan
authors.forename: M
authors.initials: M
authors.lastname: Biard-Piechaczyk
authors.forename: T D
authors.initials: TD
authors.lastname: Hua
authors.forename: Z J
authors.initials: ZJ
authors.lastname: Gu
authors.forename: M
authors.initials: M
authors.lastname: Piechaczyk
mesh_terms: D000595:Amino Acid Sequence; D000918:Antibody Specificity; D001483:Base Sequence; D001666:Binding Sites, Antibody; D003001:Cloning, Molecular; D000939:Epitopes / Q000737:chemistry / Q000276:immunology*; D015870:Gene Expression / Q000276:immunology; D005803:Genes, Immunoglobulin; D005805:Genes, MHC Class I / Q000276:immunology; D015395:Histocompatibility Antigens Class I / Q000235:genetics* / Q000276:immunology*; D006801:Humans; D007135:Immunoglobulin Variable Region / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data
chemical_list: D000939:Epitopes; D015395:Histocompatibility Antigens Class I; D007135:Immunoglobulin Variable Region