A novel antimicrobial peptide from Bufo bufo gargarizans.
A potent and structurally novel antimicrobial peptide was isolated and characterized from the stomach tissue of Bufo bufo gargarizans, an Asian toad. The 39-amino acid peptide, named buforin I, was purified to homogeneity by heparin-affinity column and reverse-phase HPLC. The amino acid sequence of buforin I was identical in 37 of 39 amino-terminal residues of Xenopus histone H2A. The buforin I showed strong antimicrobial activities in vitro against a broad-spectrum of microorganisms and was found to be more potent than magainin 2. In addition, a 21-amino acid peptide, named buforin II, which was derived from buforin I, showed more potent antimicrobial activities than buforin I.
Park
C B
CB
Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Taejeon, Korea.
Kim
M S
MS
Kim
S C
SC
D000595:Amino Acid Sequence; D000818:Animals; D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D002023:Bufo bufo; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D005658:Fungi / Q000187:drug effects; D006090:Gram-Negative Bacteria / Q000187:drug effects; D006094:Gram-Positive Bacteria / Q000187:drug effects; D006657:Histones / Q000737:chemistry; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D017386:Sequence Homology, Amino Acid; D013270:Stomach / Q000737:chemistry*; D014981:Xenopus
D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D006657:Histones; D011506:Proteins; C097846:buforin I; C097849:buforin II