title: Rotavirus shedding in feces of gnotobiotic calves orally inoculated with a commercial rotavirus-coronavirus vaccine.
abstract: The purpose of this study was to monitor by negative stain electron microscopy the shedding of rotavirus in the feces of gnotobiotic calves orally inoculated with a commercial modified live bovine rotavirus-bovine coronavirus vaccine. Negative stain electron microscopic examination detected vaccine rotavirus in only 1 of 41 daily fecal specimens collected from 3 gnotobiotic calves during the 2 weeks following oral inoculation with a US Department of Agriculture-licensed modified live bovine rotavirus-bovine coronavirus vaccine. In contrast, rotavirus was demonstrable by the same negative stain electron microscopic examination procedure in 17 of 19 fecal specimens collected from diarrheic gnotobiotic or colostrum-deprived calves during the first 8 days after inoculation with virulent bovine rotavirus field strains. Rotavirus was also detected by this procedure in 4 enzyme-linked immunosorbent assay positive fecal specimens collected from naturally-infected diarrheic dairy calves. These results suggest that fecal shedding of vaccine rotavirus demonstrable by electron microscopic examination is uncommon following oral inoculation of calves with the bovine rotavirus-bovine coronavirus vaccine.
authors.affiliation: Food Animal Health Research Program, Ohio Agricultural Research and Development Center, Ohio State University, Wooster 44691-4096, USA.
authors.forename: K W
authors.initials: KW
authors.lastname: Theil
authors.forename: C M
authors.initials: CM
authors.lastname: McCloskey
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D002417:Cattle; D003126:Colostrum; D017934:Coronavirus / Q000276:immunology / Q000302:isolation & purification* / Q000648:ultrastructure; D005243:Feces / Q000821:virology; D005856:Germ-Free Life; D007114:Immunization; D005779:Immunodiffusion; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D008854:Microscopy, Electron; D012401:Rotavirus / Q000276:immunology / Q000302:isolation & purification* / Q000648:ultrastructure; D014765:Viral Vaccines / Q000008:administration & dosage*; D017201:Virus Shedding
chemical_list: D000914:Antibodies, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D014765:Viral Vaccines