PCR as a confirmatory test for human immunodeficiency virus type 1 infection in individuals with indeterminate western blot (immunoblot) profiles.
A combination of two PCR systems which amplify human immunodeficiency virus type 1 (HIV-1) gag and pol DNA was used to elucidate the HIV-1 infection status of a group of individuals screening positive for anti-HIV but having indeterminate Western blot (immunoblot) profiles. The results obtained suggest PCR could be used to resolve such cases.
Sethoe
S Y
SY
Department of Pathology, Singapore General Hospital, Republic of Singapore.
Ling
A E
AE
Sng
E H
EH
Monteiro
E H
EH
Chan
R K
RK
D015153:Blotting, Western; D005500:Follow-Up Studies; D015683:Gene Products, gag; D015684:Gene Products, pol; D015658:HIV Infections / Q000175:diagnosis*; D015497:HIV-1 / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D016133:Polymerase Chain Reaction; D013997:Time Factors
D015683:Gene Products, gag; D015684:Gene Products, pol