title: Inhibition of staphylococcal enterotoxin-driven lymphocyte proliferation by anti-MHC class II monoclonal antibody.
abstract: Staphylococcal enterotoxins (SE) bind to major histocompatibility complex (MHC) class II molecules and the V beta region of T cell receptors (TCR) and subsequently induces T cell proliferation. This mitogenicity is the basis of pathological effects seen in food poisoning and toxic shock syndrome. Toxin-specific monoclonal antibodies have previously been shown to be effective in blocking toxin stimulated T cell responses. In this study, a monoclonal antibody, 52BL1, was found to be a potent inhibitor of SEA-, SEB-, SEC1-, SED-, and SEE-induced lymphocyte proliferation assays, which indicates that a single anti-HLA (human leukocyte antigen) class II antibody is effective in blocking the biological effects of these toxins. These results demonstrate the possibility of using anti-HLA class II antibodies in a clinical setting as an antagonist to staphylococcal enterotoxinmediated pathogenesis.
authors.affiliation: WHO Immunology Centre, Department of Microbiology, Faculty of Medicine, National University of Singapore.
authors.forename: M T
authors.initials: MT
authors.lastname: Loh
authors.forename: N
authors.initials: N
authors.lastname: Srinivasan
authors.forename: S H
authors.initials: SH
authors.lastname: Chan
authors.forename: E C
authors.initials: EC
authors.lastname: Ren
mesh_terms: D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D001667:Binding, Competitive / Q000276:immunology; D004768:Enterotoxins / Q000276:immunology*; D000949:Histocompatibility Antigens Class II / Q000276:immunology*; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects*; D013211:Staphylococcus aureus / Q000187:drug effects / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D004768:Enterotoxins; D000949:Histocompatibility Antigens Class II