Fluorescent in situ hybridization analysis of chromosomally normal gametes and abnormal arrested embryos.
OBJECTIVE
The purpose of the present study was to investigate if the arrested embryos from a couple with several previously failed IVF treatments were chromosomally normal. Probes for chromosomes X, Y and 18 were used.


RESULTS
A couple had undergone 7 in vitro fertilization treatments over a 2 1/2-year period without achieving a pregnancy. In each cycle, where fertilization was obtained, the development of the embryos was arrested. Fluorescent in situ hybridization probes for chromosomes X and Y (and 18) was carried out on gametes and on embryos in 2 separate cycles. Sperm and oocytes were normally haploid X0 or Y0. The nuclei of the blastomeres were fragmented and mosaic for X or Y, or monosomic X0, despite the fact that 2 pronuclei had been assessed on day 1 following intracytoplasmic sperm injection.


CONCLUSIONS
Chromosomally normal gametes can result in abnormal embryos manifested by arrested development and unexplained infertility.
Jakobsson
A H
AH
Fertility Center Scandinavia, Carlanderska Hospital, Göteborg, Sweden.
Hanson
C
C
Wikland
M
M
Hamberger
L
L
D000328:Adult; D002467:Cell Nucleus / Q000648:ultrastructure; D002869:Chromosome Aberrations / Q000175:diagnosis*; D025063:Chromosome Disorders; D002875:Chromosomes / Q000235:genetics*; D002887:Chromosomes, Human, Pair 18 / Q000648:ultrastructure; D015342:DNA Probes; D004622:Embryo, Mammalian / Q000002:abnormalities* / Q000648:ultrastructure; D005314:Embryonic and Fetal Development; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D008297:Male; D009865:Oocytes; D011247:Pregnancy; D013084:Sperm-Ovum Interactions; D013094:Spermatozoa / Q000002:abnormalities*; D014960:X Chromosome / Q000235:genetics; D014998:Y Chromosome / Q000235:genetics
D015342:DNA Probes