title: Crown gall culture and production of tanshinone in Salvia miltiorrhiza.
abstract: Crown galls were induced by direct infection of sterile seedlings with Agrobacterium tumefaciens C58, and the transformation was proved by opine identification. The crown galls grew well in a hormone-free medium. B5 and MS basic media were good for the growth of crown galls (strain Ca), which increased up to 102 and 90 times in a month, respectively. However, 67-V and WP basic media are good for tanshinone production. It has been shown that crown galls can be utilized as a culture system to produce secondary metabolites.
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Beijing, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Zhang
authors.forename: J
authors.initials: J
authors.lastname: Song
authors.forename: B
authors.initials: B
authors.lastname: Zhao
authors.forename: H
authors.initials: H
authors.lastname: Liu
mesh_terms: D045784:Abietanes; D016960:Agrobacterium tumefaciens / Q000235:genetics; D000900:Anti-Bacterial Agents / Q000378:metabolism*; D001120:Arginine / Q000031:analogs & derivatives / Q000032:analysis; D002478:Cells, Cultured / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000378:metabolism; D010616:Phenanthrenes / Q000378:metabolism*; D010941:Plant Tumors / Q000737:chemistry* / Q000382:microbiology / Q000648:ultrastructure; D010957:Plasmids / Q000235:genetics; D013053:Spectrophotometry; D014170:Transformation, Genetic / Q000235:genetics; D014466:Ultraviolet Rays
chemical_list: D045784:Abietanes; D000900:Anti-Bacterial Agents; D004365:Drugs, Chinese Herbal; D010616:Phenanthrenes; C021751:tanshinone; C008666:nopaline; D001120:Arginine