title: T cell receptor (TCR) V gene segment use in HLA-typed Japanese healthy subjects.
abstract: The expression of 13 different alpha and beta V gene segments of the T cell receptor for antigen (TCR) was examined, using V gene-specific MoAbs, on human peripheral blood T lymphocytes from 32 healthy Japanese subjects. In addition, to examine associations between TCR V gene products and HLA alleles, the HLA class I and class II types of all subjects were serologically determined. The reactivities of the anti-TCR V-specific MoAbs were, with some significant exceptions, similar to those previously described in healthy Caucasian subjects. We found a non-random V gene usage as well as a statistically significant bias of the expression of eight V beta gene products towards the CD4+ subpopulation, and a significant skewness in the usage of V alpha 12 towards the CD8+ population. Some subjects showed increased reactivities (above 10%) of certain MoAbs, mainly in the CD8+ subpopulation. We found no distinct correlation between any certain HLA class I or II allele and TCR V gene usage in the CD8+ or CD4+ subpopulations, respectively. In conclusion, the pattern of anti-TCR V-specific MoAb reactivities found in CD4+ and CD8+ subsets of peripheral blood lymphocytes of healthy Japanese subjects was in general found to match that previously described in healthy Caucasian subjects.
authors.affiliation: Chest Disease Research Institute, Kyoto University, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Shigematsu
authors.forename: S
authors.initials: S
authors.lastname: Nagai
authors.forename: T
authors.initials: T
authors.lastname: Mikuniya
authors.forename: T
authors.initials: T
authors.lastname: Izumi
authors.forename: H
authors.initials: H
authors.lastname: Wigzell
authors.forename: A G
authors.initials: AG
authors.lastname: Eklund
authors.forename: J
authors.initials: J
authors.lastname: Grunewald
mesh_terms: D000328:Adult; D000368:Aged; D003601:Cytotoxicity Tests, Immunologic; D005260:Female; D006680:HLA Antigens / Q000032:analysis / Q000235:genetics*; D006650:Histocompatibility Testing; D006801:Humans; D007564:Japan; D008297:Male; D008875:Middle Aged; D005810:Multigene Family; D011948:Receptors, Antigen, T-Cell / Q000235:genetics*
chemical_list: D006680:HLA Antigens; D011948:Receptors, Antigen, T-Cell