title: Comparative analysis of two assays for genotyping hepatitis C virus based on genotype-specific primers or probes.
abstract: OBJECTIVE
  The importance of determining the hepatitis C virus genotype has increased since several authors have described a good correlation between hepatitis C virus genotype and response to interferon treatment or to disease severity. It is thus of particular importance to develop reliable assays for hepatitis C virus genotyping.
  
  
  METHODS
  We have comparatively analyzed hepatitis C virus genotypes of 208 French and Italian chronically infected patients using genotype-specific primers polymerase chain reaction in the capsid region and a genotype-specific probe-based assay in the 5' untranslated region (LiPA).
  
  
  RESULTS
  We found a good concordance between the two assays for the prevalent genotypes in our regions (145/174). The nucleotide sequences in the 5'UTR and capsid domain were investigated to determine the molecular basis of some discordant results. This analysis showed that the genotype-specific probe-based assay gave more consistent results, probably because this technique is based on several probes distributed along the 5'UTR to reveal each genotype. In contrast, the low variability of 5'UTR did not always permit classification of the hepatitis C virus genotype 1 subtypes. We also found some problems, using genotype-specific primers polymerase chain reaction, for less represented genotypes; in particular, in the presence of type 2a/III we obtained more discordant results. This observation is of importance in view of the potential association of this genotype with mild liver disease and a good response to interferon-a treatment.
authors.affiliation: CBMS, Institut Pasteur, CHU Necker, Paris, France.
authors.forename: C
authors.initials: C
authors.lastname: Giannini
authors.forename: V
authors.initials: V
authors.lastname: Thiers
authors.forename: J B
authors.initials: JB
authors.lastname: Nousbaum
authors.forename: L
authors.initials: L
authors.lastname: Stuyver
authors.forename: G
authors.initials: G
authors.lastname: Maertens
authors.forename: C
authors.initials: C
authors.lastname: Bréchot
mesh_terms: D001483:Base Sequence; D002213:Capsid / Q000235:genetics*; D002908:Chronic Disease; D005838:Genotype; D016174:Hepacivirus / Q000235:genetics* / Q000302:isolation & purification; D006526:Hepatitis C / Q000235:genetics / Q000821:virology*; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000032:analysis*
chemical_list: D012367:RNA, Viral