title: The expression of myristoylated alanine-rich C-kinase substrate in oligodendrocytes is developmentally regulated.
abstract: Myristoylated alanine-rich C-kinase substrate (MARCKS), a major substrate of activated protein kinase C (PKC), is thought to be involved in PKC-mediated signal transduction events. In the present study, we have examined the expression of MARCKS in primary cultures of rat glial cells. Western blot analysis of different glial cell types (i.e., astrocytes, oligodendrocytes and microglia) revealed a relatively high level of MARCKS protein in oligodendrocytes. MARCKS protein and MARCKS mRNA levels in oligodendrocytes increased with time in culture, indicating a developmental regulation in MARCKS gene expression in differentiating oligodendrocytes. Immunocytochemical examination of developing oligodendrocytes indicated a strong labeling of MARCKS distributed both in the cell body and in the lacy network of processes. These findings, in concert with our previous observations on the role of PKC in oligodendrogenesis, strongly implicate a PKC-signaling system in oligodendrocyte development.
authors.affiliation: Department of Neurology, Medical University of South Carolina, Charleston 29425, USA.
authors.forename: N R
authors.initials: NR
authors.lastname: Bhat
authors.forename: P
authors.initials: P
authors.lastname: Zhang
authors.forename: A N
authors.initials: AN
authors.lastname: Bhat
mesh_terms: D000818:Animals; D015153:Blotting, Western; D001921:Brain / Q000166:cytology / Q000254:growth & development*; D002454:Cell Differentiation; D002478:Cells, Cultured; D007150:Immunohistochemistry; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D008856:Microscopy, Fluorescence; D009186:Myelin Sheath / Q000378:metabolism; D000076250:Myristoylated Alanine-Rich C Kinase Substrate; D009836:Oligodendroglia / Q000166:cytology / Q000378:metabolism*; D010766:Phosphorylation; D014176:Protein Biosynthesis; D011493:Protein Kinase C / Q000096:biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D015398:Signal Transduction; D013234:Stem Cells / Q000378:metabolism
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; C000619712:Marcks protein, rat; D008565:Membrane Proteins; D012333:RNA, Messenger; D000076250:Myristoylated Alanine-Rich C Kinase Substrate; D011493:Protein Kinase C