Salmonella typhimurium infection in mice: lymphoproliferative responses to fractionated protein antigens.
The proliferative response of spleen cells of mice immunized with S. typhimurium by the oral route was analyzed using antigen fractions from a protein extract of the bacteria. Mice that survived the challenge with a virulent strain of Salmonella and normal mice were also studied. Mice were immunized with three doses of live S. typhimurium on consecutive days (3C) or once a week for 3 weeks (3W). Fractions 12-100 kDa from the protein extract were separated by SDS-polyacrylamide gel electrophoresis, electroblotted to nitrocellulose membranes and processed to obtain particulate antigens for use in lymphocyte cultures. Mice immunized weekly showed a higher survival rate and responded to more antigenic fractions. We identified three fractions of 68-76, 50-52, and 42-45 kDa that were immunodominant for spleen cells from S. typhimurium immunized mice and from survivors to the challenge with the virulent strain.
Melendro
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EI
Departamento de Medicina Experimental, Facultad de Medicina, Universidad Nacional Autónoma de México, México, D.F.
Calderón
G M
GM
Pérez
C
C
Ximénez
C
C
D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis / Q000494:pharmacology*; D007114:Immunization; D008213:Lymphocyte Activation / Q000187:drug effects* / Q000276:immunology; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D012481:Salmonella Infections, Animal / Q000276:immunology*; D012486:Salmonella typhimurium / Q000276:immunology*; D013154:Spleen / Q000187:drug effects / Q000276:immunology
D000942:Antigens, Bacterial