title: Intra-abdominal sepsis impairs colonic reparative collagen synthesis.
abstract: BACKGROUND
  Intra-abdominal infection is generally considered a contraindication to primary colon anastomosis. In order to elucidate the mechanisms by which sepsis affects colonic healing, we studied anastomotic new collagen and protein synthesis and collagen gene expression in a relevant animal model.
  
  
  METHODS
  Forty male Sprague-Dawley rats (240 to 260 g) underwent sham laparotomy (SHAM, n = 18) or cecal ligation and single puncture (CLP, n = 22). After 24 hours, animals underwent single-layer left colon anastomosis. Animals were sacrificed either 1 or 4 days postanastomosis. Anastomotic segments of colon were excised, minced, and incubated with 4.5 muCi 3H-proline. After 3 hours, tissue 3H-proline incorporation was quantitated as an index of total new protein synthesis. The protein fraction was then digested with purified collagenase enzyme to determine 3H-proline incorporation into collagenase-digestible protein, an index of new collagen synthesis. Total RNA was extracted from anastomotic tissue samples and subjected to Northern blot analysis for type I and type III collagen genes.
  
  
  RESULTS
  Intra-abdominal sepsis resulted in markedly less new collagen synthesis 1 day postanastomosis (9,163 +/- 1,234 versus 3,744 +/- 444 disintegrates per minute 3H-proline/mg of protein, P < 0.0001) and 4 days postanastomosis (8,462 +/- 956 versus 5,708 +/- 802 dpm/mg of protein P < 0.05). Noncollagenous protein synthesis was also impaired in anastomotic tissue from CLP rats on postanastomosis day 1 (37,497 +/- 3,740 versus 18,593 +/- 2,695 dpm/mg of of protein, P < 0.001) and postanastomosis day 4 (28,238 +/- 834 versus 17,784 +/- 1,415 dpm/mg of of protein, P < 0.0001). The expression of type I and type III collagen was altered relative to the normal temporal sequence observed in SHAM animals.
  
  
  CONCLUSIONS
  Intra-abdominal infection impairs colonic reparative collagen and protein synthesis. In addition, regulation of type I and type III collagen genes is altered by intra-abdominal sepsis, and the alteration likely contributes to impaired new collagen synthesis and decreased colonic mechanical strength.
authors.affiliation: Department of Surgery, Sinai Hospital of Baltimore, Maryland 21215, USA.
authors.forename: G M
authors.initials: GM
authors.lastname: Ahrendt
authors.forename: U S
authors.initials: US
authors.lastname: Tantry
authors.forename: A
authors.initials: A
authors.lastname: Barbul
mesh_terms: D000005:Abdomen; D000714:Anastomosis, Surgical; D000818:Animals; D015152:Blotting, Northern; D003094:Collagen / Q000096:biosynthesis* / Q000235:genetics; D003106:Colon / Q000378:metabolism* / Q000601:surgery*; D004195:Disease Models, Animal; D015870:Gene Expression; D007239:Infections / Q000378:metabolism*; D008297:Male; D011392:Proline / Q000378:metabolism; D014176:Protein Biosynthesis; D012313:RNA / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D014945:Wound Healing / Q000502:physiology
chemical_list: D012313:RNA; D003094:Collagen; D011392:Proline