title: Red blood cell cation transports in uraemic anaemia: evidence for an increased K/Cl co-transport activity. Effects of dialysis and erythropoietin treatment.
abstract: This study examines the role of uraemia and the effect of different dialysis treatments on red cell cation transport. We evaluated the main cation transport systems in erythrocytes of non-dialysed end-stage renal disease (ESRD) subjects, of patients undergoing haemodialysis (HD) and continuous ambulatory peritoneal dialysis (CAPD), as well as the changes induced by human recombinant erythropoietin (r-HuEPO) administration. In uraemic undialysed and dialysed patients, we observed an increase in K/Cl co-transport activity and in shrinkage-induced amiloride-sensitive (HMA-sensitive) Na efflux (Na/H exchange) and a decrease in Na/K pump and Na/K/Cl co-transport activity, while Na/Li exchange was increased only in dialysed patients. In uraemic erythrocytes, we showed for the first time an increased K/Cl co-transport activity, which was cell age independent. Generally, the different method of dialysis (CAPD or HD) did not modify the cation transport abnormalities observed. During the treatment with r-HuEPO, all the systems, with the exception of the Na/K pump and Na/K/Cl co-transport, increased their activities following the increase of circulating young red cells. The changes produced under r-HuEPO administration were transient and cation transports returned to the baseline values within 100 days of treatment, indicating a primary and prominent pathogenetic role of uraemia in modulating the red cell membrane cation transport activities.
authors.affiliation: Department of Internal Medicine, University of Verona, Italy.
authors.forename: L
authors.initials: L
authors.lastname: De Franceschi
authors.forename: O
authors.initials: O
authors.lastname: Olivieri
authors.forename: D
authors.initials: D
authors.lastname: Girelli
authors.forename: A
authors.initials: A
authors.lastname: Lupo
authors.forename: P
authors.initials: P
authors.lastname: Bernich
authors.forename: R
authors.initials: R
authors.lastname: Corrocher
mesh_terms: D000740:Anemia / Q000097:blood / Q000209:etiology / Q000628:therapy; D002712:Chlorides / Q000378:metabolism*; D004912:Erythrocytes / Q000378:metabolism*; D004921:Erythropoietin / Q000627:therapeutic use*; D006801:Humans; D017136:Ion Transport; D007676:Kidney Failure, Chronic / Q000150:complications; D010531:Peritoneal Dialysis, Continuous Ambulatory; D011188:Potassium / Q000378:metabolism*; D006435:Renal Dialysis; D017582:Renal Replacement Therapy; D012964:Sodium / Q000378:metabolism*; D014511:Uremia / Q000097:blood* / Q000209:etiology / Q000628:therapy
chemical_list: D002712:Chlorides; D004921:Erythropoietin; D012964:Sodium; D011188:Potassium