Inactivation of Trypanosoma cruzi trypomastigote forms in blood components by photodynamic treatment with phthalocyanines.
Three phthalocyanine dyes HOSiPcOSi(CH3)2(CH2)3N(CH3)2 (Pc 4), HOSiPcOSi(CH3)2(CH2)3N+(CH3)3I- (Pc 5) and aluminum tetrasulfophthalocyanine hydroxide (AlOHPcS4) were evaluated for their ability to inactivate the trypomastigote form of Trypanosoma cruzi in fresh frozen plasma (FFP) and red blood cell concentrates (RBCC). The compound Pc 4 was found to be highly effective in killing T. cruzi, Pc 5 less effective and AlOHPcS4 ineffective. With FFP as the medium, a complete loss of parasite infectivity in vitro (> or = 5 log10) was found to occur with 2 microM Pc 4 after irradiation with red light (> 600 nm) at a fluence of 7.5 J/cm2, while with RBCC as the medium, a complete loss was found to occur at a fluence of 15 J/cm2. Even without illumination, Pc 4 at 2 microM also killed about 3.7-4.1 log10 of T. cruzi in FFP during 30 min. Observed differences in T. cruzi killing by the various phthalocyanines may related to differences in binding; Pc 4 binds to the parasites about twice as much as Pc 5. Ultrastructural analysis of treated parasites suggests that mitochondria are a primary target of this photodynamic treatment. The data indicate that Pc 4 combined with exposure to red light could be used to eliminate bloodborne T. cruzi parasites from blood components intended for transfusion. The inactivation of T. cruzi by Pc 4 in the dark suggests a possible therapeutic application.
Gottlieb
P
P
Lindsley F. Kimball Research Institute of the New York Blood Center, New York, NY 10021, USA.
Shen
L G
LG
Chimezie
E
E
Bahng
S
S
Kenney
M E
ME
Horowitz
B
B
Ben-Hur
E
E
D000818:Animals; D014355:Chagas Disease / Q000469:parasitology / Q000517:prevention & control / Q000635:transmission; D004396:Coloring Agents; D006801:Humans; D066298:In Vitro Techniques; D007211:Indoles; D054833:Isoindoles; D018512:Parasitemia / Q000628:therapy; D010778:Photochemotherapy; D011838:Radiation-Sensitizing Agents; D065227:Transfusion Reaction; D014349:Trypanosoma cruzi / Q000187:drug effects* / Q000254:growth & development / Q000528:radiation effects*
D004396:Coloring Agents; D007211:Indoles; D054833:Isoindoles; D011838:Radiation-Sensitizing Agents; C013647:phthalocyanine