Human immunoglobulin production in SCID mice following primary sensitisation of human lymphocytes in situ.
We have grafted human peripheral blood mononuclear cells to the peritoneal cavity of SCID mice, exposed them to soluble antigens in the absence of adjuvant and examined their ability to make specific antibody. The effect of in vitro pre-priming of donor cells on antibody production was determined. Human IgM and IgG antibodies were found in the SCID serum following in situ antigenic challenge and there was good evidence of antigen specificity. The total Ig levels and the relative antigen binding ability were diminished by in vitro pre-priming. The results demonstrate that primary human antibodies against antigens to which the donor is probably naive can be generated by in situ sensitisation in SCID mice, but that optimisation for each antigen may be required.
Kurpisz
M
M
Institute of Human Genetics, Polish Academy of Sciences, Poznań.
Walker
W
W
Field
M
M
Gallagher
G
G
D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis; D000912:Antibodies, Neoplasm / Q000096:biosynthesis; D000917:Antibody Formation; D000918:Antibody Specificity; D000942:Antigens, Bacterial / Q000494:pharmacology; D000951:Antigens, Neoplasm / Q000494:pharmacology; D016913:Blood Component Transfusion; D002689:Chlamydia / Q000276:immunology; D005260:Female; D006801:Humans; D007114:Immunization; D007136:Immunoglobulins / Q000096:biosynthesis* / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology* / Q000378:metabolism*; D008297:Male; D051379:Mice; D016513:Mice, SCID; D010051:Ovarian Neoplasms / Q000276:immunology; D013094:Spermatozoa / Q000276:immunology
D000907:Antibodies, Bacterial; D000912:Antibodies, Neoplasm; D000942:Antigens, Bacterial; D000951:Antigens, Neoplasm; D007136:Immunoglobulins