[Study of anti-tumor effects of hyperthermia combined with hydralazine on experimental tumor].
We analyzed tumor growth delay in experimental tumors after hyperthermia alone, hydralazine (HDZ) injection alone and the combination of these modalities. We also analyzed the energy parameter (ATP/Pi ratio) obtained by 31P-MRS (magnetic resonance spectroscopy). The purpose of this study was to evaluate the usefulness of 31P-MRS as an index of anti-tumor effect. FM3A tumor cells were transplanted subcutaneously in the hind legs of C3H/He mice. We dipped the tumors into a heated circulating water bath. 31P-MRS was performed with a CSI spectrometer. The anti-tumor effect obtained with HDZ alone was insignificant, but combined treatment with hyperthermia and HDZ had a significant synergistic effect. The ATP/Pi ratios for all groups treated separately with HDZ or hyperthermia were not different from the control, but the ATP/Pi ratio decreased after combined use of these agents. There was a significant correlation between the decrease in ATP/Pi ratio and tumor growth delay. We observed a direct relation between the delay in tumor growth and the decline in ATP/Pi ratio after combined treatment with HDZ and hyperthermia. The ATP/Pi ratio 24 hr after treatment may be useful in predicting the efficacy of the combined use of HDZ and hyperthermia.
Aoki
Y
Y
Department of Radiology, Kansai Medical University.
Akagi
K
K
Kitada
N
N
Tanaka
Y
Y
Fritz-Zieroth
B
B
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D003131:Combined Modality Therapy; D004734:Energy Metabolism; D006830:Hydralazine / Q000627:therapeutic use*; D006979:Hyperthermia, Induced; D017078:Laser-Doppler Flowmetry; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000098:blood supply / Q000378:metabolism / Q000628:therapy*; D012039:Regional Blood Flow
D006830:Hydralazine; D000255:Adenosine Triphosphate