title: Intercellular Ca2+ waves sustain coordinate insulin secretion in pig islets of Langerhans.
abstract: Insulin release was investigated in parallel with changes in cytosolic calcium concentration, [Ca2+]i, in pig islets stimulated by glucose. After two days in culture, glucose stimulation failed to induce insulin release, and caused limited [Ca2+]i changes in few cells. After ten days, insulin response was partially restored and [Ca2+]i recordings revealed a slow oscillatory activity of the whole islet. Slow oscillations appeared to be due to the average [Ca2+]i variations resulting from the spreading of waves throughout the islet. These waves demonstrate the reestablishment of functional cell coupling, which appears to play a critical role in insulin release.
authors.affiliation: Medicine Department, DIBIT, San Raffaele Scientific Inst., Milano, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Bertuzzi
authors.forename: D
authors.initials: D
authors.lastname: Zacchetti
authors.forename: C
authors.initials: C
authors.lastname: Berra
authors.forename: C
authors.initials: C
authors.lastname: Socci
authors.forename: G
authors.initials: G
authors.lastname: Pozza
authors.forename: A E
authors.initials: AE
authors.lastname: Pontiroli
authors.forename: F
authors.initials: F
authors.lastname: Grohovaz
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism; D005456:Fluorescent Dyes; D016257:Fura-2; D005947:Glucose / Q000494:pharmacology; D066298:In Vitro Techniques; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000187:drug effects / Q000378:metabolism*; D007700:Kinetics; D008856:Microscopy, Fluorescence; D018715:Microscopy, Video; D009991:Oscillometry; D013552:Swine
chemical_list: D005456:Fluorescent Dyes; D007328:Insulin; D005947:Glucose; D002118:Calcium; D016257:Fura-2