title: Laminins 2 and 4 are expressed by human decidual cells.
abstract: During pregnancy, the resident stromal cells of the endometrium differentiate to become decidual cells and produce a pericellular basement membrane. We used immunofluorescence and Western blotting with a panel of monoclonal Ab specific for various laminin subunits to examine the composition of decidual laminin. The stromal cell basement membrane contained subunits alpha 2 (M), beta 1 (B1), beta 2 (S), and gamma 1 (B2). Low levels of alpha 1 could also be detected. The glandular and vascular basement membranes of decidual tissue contained subunits alpha 1 (A), beta 1, and gamma 1. An extract was produced from decidual extracellular matrix. Western blots of nonreducing gels showed the presence of high molecular weight complexes containing alpha 2, beta 1, beta 2, and gamma 1. These data indicated that laminins 2 and 4 are coexpressed by decidual cells. Laminin 1 was present in the extract as a minor component. In contrast, cultured stromal cells expressed laminin 1 as the major secreted variant. Immunolocalization was carried out using tissue from various stages of the nonpregnant cycle. The alpha 2 chain polypeptide was absent in the proliferative phase of the cycle but present in late secretory phase in perivascular areas where predecidual differentiation occurs. Reverse transcriptase-PCR experiments confirmed the presence of alpha 2 chain mRNA in decidua but showed that this transcript is detectable throughout the nonpregnant cycle. The results showed that laminins 2 and 4 are hormonally regulated products of decidual cells. The composition of the vascular and epithelial basement membranes remained constant throughout the cycle.
authors.affiliation: Department of Obstetrics and Gynaecology, University of Manchester, United Kingdom.
authors.forename: H J
authors.initials: HJ
authors.lastname: Church
authors.forename: L M
authors.initials: LM
authors.lastname: Vićovac
authors.forename: J D
authors.initials: JD
authors.lastname: Williams
authors.forename: N A
authors.initials: NA
authors.lastname: Hey
authors.forename: J D
authors.initials: JD
authors.lastname: Aplin
mesh_terms: D015153:Blotting, Western; D002478:Cells, Cultured; D003656:Decidua / Q000737:chemistry / Q000166:cytology / Q000378:metabolism*; D004717:Endometrium / Q000737:chemistry / Q000166:cytology / Q000378:metabolism; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007797:Laminin / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D010455:Peptides / Q000378:metabolism; D011247:Pregnancy; D012333:RNA, Messenger / Q000032:analysis; D017154:Stromal Cells / Q000378:metabolism
chemical_list: D007797:Laminin; D010455:Peptides; D012333:RNA, Messenger