title: Biomaterial-induced dysfunction in the capacity of rabbit alveolar macrophages to kill Staphylococcus epidermidis RP12.
abstract: The effect of poly(methyl methacrylate) (PMMA), titanium alloy, and silicone discs on the capacity of rabbit alveolar macrophages (AM) to kill RP12 strain of Staphylococcus epidermidis (RP12) was studied in vitro. When freshly harvested AM were preincubated with PMMA discs for 3 h and subsequently assayed for RP12 killing, there was no change in the RP12 killing capacity of AM. However, when AM were incubated with PMMA discs for 6 or 18 h at 37 degrees C in 5% CO2, the RP12 killing capacity of AM was reduced to 15% and 4%, respectively. Preincubation of AM with titanium alloy for 6 h reduced RP12 killing capacity of AM to 30%, and to 21% in 18-h incubation. Silicone discs did not affect the RP12 killing by AM at 6 h of preincubation, but reduced RP12 killing (35%) by AM when preincubated for 18 h. Preincubation of AM with PMMA discs for 3 or 6 h did not affect the level of PMA-elicited oxidative burst of AM as measured by a luminol-enhanced chemiluminescent assay. Superoxide dismutase, which eliminated the oxidative burst of AM by 90%, did not affect the RP12 killing by AM.
authors.affiliation: Medical Sciences Research Institute, Herndon, Virginia 22070, USA.
authors.forename: G
authors.initials: G
authors.lastname: Giridhar
authors.forename: Q N
authors.initials: QN
authors.lastname: Myrvik
authors.forename: A G
authors.initials: AG
authors.lastname: Gristina
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000633:toxicity*; D066298:In Vitro Techniques; D008163:Luminescent Measurements; D016676:Macrophages, Alveolar / Q000187:drug effects* / Q000276:immunology*; D008768:Methylmethacrylates / Q000633:toxicity; D010587:Phagocytosis / Q000187:drug effects*; D011817:Rabbits; D016897:Respiratory Burst / Q000187:drug effects; D012828:Silicones / Q000633:toxicity; D013212:Staphylococcus epidermidis; D013482:Superoxide Dismutase / Q000494:pharmacology; D014025:Titanium / Q000633:toxicity
chemical_list: D001672:Biocompatible Materials; D008768:Methylmethacrylates; D012828:Silicones; D014025:Titanium; D013482:Superoxide Dismutase