Limited T-cell receptor V gene usage in inclusion body myositis.
Inclusion body myositis (IBM) is a chronic, progressive inflammatory myopathy. The inflammatory infiltrates are dominated by T cells, which frequently invade muscle fibres. The present study was performed to characterize the usage of the variable (V) segment of the T-cell receptor of muscle infiltrating cells in IBM. Using the reverse transcriptase polymerase chain reaction (RT-PCR) technique the authors analysed the expression of 22 V alpha and 24 V beta families in muscle tissue from six patients with IBM displaying intense inflammatory cell infiltration. The following V alpha/V beta families appeared in at least 50% of the patients: V alpha 1, 5, 7, 15, 16, 17, 20, 21, 22 and V beta 3, 5.2, 8, 12, 14, 22. In all patients V alpha 7, 16 and V beta 8 were expressed in muscle tissue. Furthermore, in two of the patients peripheral blood lymphocytes (PBL) were investigated in parallel. There was a restricted usage of V alpha and V beta families in muscle in comparison to PBL, indicating a selective homing or local proliferation of T lymphocytes in the inflammatory lesions in IBM.
Fyhr
I M
IM
Department of Pathology, Göteborg University, Sahlgrenska Hospital, Gothenburg, Sweden.
Moslemi
A R
AR
Tarkowski
A
A
Lindberg
C
C
Oldfors
A
A
D000328:Adult; D000368:Aged; D001483:Base Sequence; D015139:Blotting, Southern; D004247:DNA / Q000032:analysis; D017931:DNA Primers / Q000737:chemistry; D005260:Female; D015870:Gene Expression; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000166:cytology / Q000648:ultrastructure; D018979:Myositis, Inclusion Body / Q000276:immunology* / Q000473:pathology; D016133:Polymerase Chain Reaction; D012194:RNA-Directed DNA Polymerase; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics*; D013601:T-Lymphocytes / Q000276:immunology*
D017931:DNA Primers; D016693:Receptors, Antigen, T-Cell, alpha-beta; D004247:DNA; D012194:RNA-Directed DNA Polymerase