[Functional activity of T-, B-lymphocytes, and macrophages during suppression of expression of the env gene from an endogenous retrovirus genome].
Incubation of murine spleen cells with antisense oligonucleotide complementary to initiation site of this gene highly increased RNA synthesis relative to the normal T- and B-lymphocytes from spleen. In macrophages, inhibition of gene env expression stimulated phagocytosis and IL-1 production. Under these conditions, the level of expression of proviral envelope transmembrane p15E protein, which in infectious type C retroviruses is known to be immunosuppressive, decreased in spleen cells. Antisense oligonucleotide stimulatory effect on murine spleen cell RNA synthesis is presumably related to the reduced production of endogenous p15E.
Gaĭdul'
K V
KV
Chernukhin
I V
IV
Khaldoianidi
S K
SK
Svinarchuk
F P
FP
Vlasov
V V
VV
Kozlov
V A
VA
D000595:Amino Acid Sequence; D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D001483:Base Sequence; D002478:Cells, Cultured; D015752:Genes, env; D007375:Interleukin-1 / Q000096:biosynthesis; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008908:Mink Cell Focus-Inducing Viruses / Q000235:genetics* / Q000276:immunology; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D010587:Phagocytosis / Q000235:genetics; D012367:RNA, Viral / Q000096:biosynthesis; D013154:Spleen / Q000166:cytology / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
D007375:Interleukin-1; D009838:Oligodeoxyribonucleotides; D012367:RNA, Viral
Funktsional'naia aktivnost' T-, B-limfotsitov i makrofagov v usloviiakhpodavleniia ékspressii gena env éndogennogo retrovirusnogo genoma.