title: Activation of two Cynops genes, fork head and sonic hedgehog, in animal cap explants.
abstract: We have isolated cDNAs of sonic hedgehog (shh) and fork head from Cynops (Japanese newt) embryo. Their expression was investigated in relation to mesoderm induction by activin and basic fibroblast growth factor (bFGF). Different from these homologs in Xenopus, they are activated not only by activin but also by bFGF in animal cap explants, showing a difference of animal pole cells in responsiveness to bFGF between Cynops and Xenopus. We also investigated the involvement of fork head in shh activation. The expression of shh was activated in animal caps which overexpressed either of two Xenopus fork head homologs, pintallavis/XFD-1 or XFKH-1/XFD-1', indicating that fork head up-regulates the transcription of shh in Cynops embryo.
authors.affiliation: Radioisotope Research Center, Nagoya University, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Takabatake
authors.forename: T C
authors.initials: TC
authors.lastname: Takahashi
authors.forename: K
authors.initials: K
authors.lastname: Inoue
authors.forename: M
authors.initials: M
authors.lastname: Ogawa
authors.forename: K
authors.initials: K
authors.lastname: Takeshima
mesh_terms: D028341:Activins; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers; D004625:Embryo, Nonmammalian / Q000502:physiology*; D004627:Embryonic Induction / Q000502:physiology*; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D051858:Forkhead Transcription Factors; D005786:Gene Expression Regulation / Q000187:drug effects; D006133:Growth Substances / Q000494:pharmacology; D053823:Hedgehog Proteins; D007265:Inhibins / Q000494:pharmacology; D008648:Mesoderm / Q000187:drug effects / Q000502:physiology; D051379:Mice; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000096:biosynthesis* / Q000235:genetics; D009865:Oocytes / Q000502:physiology; D016133:Polymerase Chain Reaction; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis; D012455:Salamandridae / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D015534:Trans-Activators; D014157:Transcription Factors / Q000096:biosynthesis* / Q000235:genetics; D014158:Transcription, Genetic; D014981:Xenopus
chemical_list: D017931:DNA Primers; D051858:Forkhead Transcription Factors; D006133:Growth Substances; D053823:Hedgehog Proteins; D009687:Nuclear Proteins; D011506:Proteins; D011994:Recombinant Proteins; D015534:Trans-Activators; D014157:Transcription Factors; D016222:Fibroblast Growth Factor 2; D028341:Activins; D007265:Inhibins