A new monoclonal antibody (3D3) generated with human respiratory mucins and directed against Lewis determinants.
We have prepared a monoclonal antibody (MAb), 3D3, raised against purified human respiratory mucins. This antibody recognized mucins and proteolytically derived glycopeptides. The epitope recognized by the antibody was destroyed by alpha-L-fucosidase, indicating that it was present on the carbohydrate moieties. Structural specificity was determined by adsorption on a variety of synthetic, insolubilized oligosaccharides. Several lines of evidence indicate that the 3D3 MAb reacted strongly with the Lewis (Leb) antigen, but also recognized Le(a) and Le(y) determinants. This antibody might be useful to study mucin secretion.
Emery
N
N
Unité INSERM no. 377, Lille, France.
Palfaï
S B
SB
Place
G
G
Oriol
R
R
Hall
R L
RL
Roussel
P
P
Lhermitte
M
M
D000327:Adsorption; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology; D001666:Binding Sites, Antibody; D015153:Blotting, Western; D001980:Bronchi / Q000276:immunology* / Q000378:metabolism; D002240:Carbohydrate Sequence; D004797:Enzyme-Linked Immunosorbent Assay; D006031:Glycosylation; D006386:Hemagglutination Tests; D006801:Humans; D007150:Immunohistochemistry; D007983:Lewis Blood Group Antigens / Q000276:immunology*; D008969:Molecular Sequence Data; D009077:Mucins / Q000276:immunology*
D000911:Antibodies, Monoclonal; D007983:Lewis Blood Group Antigens; D009077:Mucins