title: Changes in serum antioxidant concentrations during infection with caprine lentivirus.
abstract: We are currently using caprine arthritis encephalitis virus (CAEV) infection in goats as a model to understand changes in some clinical parameters and host response to infection with human immunodeficiency virus (HIV). The objective of this study was to measure changes in serum antioxidant activities in various age groups of goats infected with CAEV. Serum from CAEV-infected goats had significantly higher catalase activity (105.47 +/- 5.96 kU/l) than serum from healthy control goats (79.92 +/- 17.06 kU/l). Moreover, serum catalase activity increased with increase in the time after infection with CAEV. No change was observed in total superoxide dismutase (SOD) or glutathione peroxidase activity although CuZn SOD levels were elevated in infected goats. There was a positive correlation between serum catalase activity and hydrogen peroxide (H2O2) scavenging activity (r = 0.70, p < 0.05). In order to investigate cell membrane integrity, we determined lactate dehydrogenase (LDH) activity in infected goats. Although there was a transient increase in LDH no correlation was observed between increased serum catalase activity and LDH activity (r = 0.16, p > 0.05). We have earlier observed decreased oxyradical production in CAEV infected goats. This observed increase in serum catalase, a scavenger of endogenous free radicals such as H2O2 may be partly responsible for the observed decrease in oxygen radicals found in vivo.
authors.affiliation: Center for Biomedical Research/RCMI, Carver Research Foundation, Tuskegee University, Alabama 36088, USA.
authors.forename: E G
authors.initials: EG
authors.lastname: Mdurvwa
authors.forename: P O
authors.initials: PO
authors.lastname: Ogunbiyi
authors.forename: P G
authors.initials: PG
authors.lastname: Reddy
authors.forename: H S
authors.initials: HS
authors.lastname: Gakou
authors.forename: S O
authors.initials: SO
authors.lastname: Sodeke
authors.forename: A J
authors.initials: AJ
authors.lastname: Carty
mesh_terms: D000367:Age Factors; D000818:Animals; D000975:Antioxidants / Q000032:analysis*; D001170:Arthritis, Infectious / Q000201:enzymology / Q000662:veterinary* / Q000821:virology; D016088:Arthritis-Encephalitis Virus, Caprine; D002100:Cachexia / Q000201:enzymology / Q000662:veterinary / Q000821:virology; D002374:Catalase / Q000097:blood*; D004195:Disease Models, Animal; D016166:Free Radical Scavengers; D005979:Glutathione Peroxidase / Q000097:blood*; D015511:Goat Diseases / Q000097:blood*; D006041:Goats / Q000097:blood; D015658:HIV Infections; D006861:Hydrogen Peroxide / Q000097:blood; D007770:L-Lactate Dehydrogenase / Q000097:blood; D016180:Lentivirus Infections / Q000201:enzymology / Q000662:veterinary*; D013482:Superoxide Dismutase / Q000097:blood*
chemical_list: D000975:Antioxidants; D016166:Free Radical Scavengers; D006861:Hydrogen Peroxide; D007770:L-Lactate Dehydrogenase; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase