Augmented immunological activities of recombinant lipopolysaccharide possessing the mannose homopolymer as the O-specific polysaccharide.
Recombinant lipopolysaccharide possessing the mannose homopolymer as the O-specific polysaccharide was manufactured genetically by transforming Escherichia coli K-12 with various rfb genes capable of synthesizing the mannose homopolymer. Recombinant lipopolysaccharide exhibited levels of anticomplement activity, adjuvant activity, and regional lymph node-enlarging activity much higher than those exhibited by the original rough-type lipopolysaccharide from E. coli K-12 or lipopolysaccharide possessing the heteropolysaccharide from E. coli O111. Immunological activities of recombinant lipopolysaccharide were as strong as those of wild-type lipopolysaccharide possessing the mannose homopolymer. Characteristic activities of wild-type lipolysaccharide possessing the mannose homopolymer were exhibited by recombinant lipopolysaccharide. The abilities of lipopolysaccharide to activate B cells polyclonally and to produce cytokines did not seem to be related to the presence of the mannose homopolymer. Therefore, it was suggested that the mannose homopolymer in the O-specific polysaccharide might exclusively enhance anticomplement activity, adjuvant activity, and regional lymph node-enlarging activity among various lipid A activities.
Paeng
N
N
Department of Natural Science Informatics, School of Informatics and Sciences, Nagoya University, Aichi, Japan.
Kido
N
N
Schmidt
G
G
Sugiyama
T
T
Kato
Y
Y
Koide
N
N
Yokochi
T
T
D000276:Adjuvants, Immunologic; D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D016667:Bacterial Capsules; D002240:Carbohydrate Sequence; D003169:Complement Inactivator Proteins; D004926:Escherichia coli / Q000235:genetics / Q000276:immunology*; D005818:Genetic Engineering; D007371:Interferon-gamma / Q000097:blood; D008070:Lipopolysaccharides / Q000276:immunology*; D008198:Lymph Nodes / Q000276:immunology / Q000473:pathology; D008351:Mannans / Q000276:immunology*; D051379:Mice; D008969:Molecular Sequence Data; D019081:O Antigens / Q000276:immunology*; D011135:Polysaccharides, Bacterial / Q000276:immunology; D012710:Serum Albumin, Bovine; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis
D000276:Adjuvants, Immunologic; D003169:Complement Inactivator Proteins; D008070:Lipopolysaccharides; D008351:Mannans; D019081:O Antigens; D011135:Polysaccharides, Bacterial; D014409:Tumor Necrosis Factor-alpha; C012749:anticomplement; C050671:capsular polysaccharide K12; D012710:Serum Albumin, Bovine; C097323:polymannose; D007371:Interferon-gamma