title: Wheat bran suppresses potato starch--potentiated colorectal tumorigenesis at the aberrant crypt stage in a rat model.
abstract: OBJECTIVE
  Starch that escapes digestion in the small intestine (resistant starch [RS]) may act similarly to nonstarch polysaccharides in the colon. The aim of this study was to examine the effect of raw potato starch alone and in combination with wheat bran on tumor development and precancer events in a rat model of colorectal cancer.
  
  
  METHODS
  Three groups of rats received either a low-RS/low-fiber ("basic") diet, the basic diet containing raw potato starch as 20% of carbohydrate content, or the potato starch diet plus 10% of "wheat bran" fiber. Epithelial proliferation, aberrant crypt foci (ACF), and tumors were measured 6 and 20 weeks after a 10-week course of dimethylhydrazine.
  
  
  RESULTS
  Rats on the potato starch diet had tumors more frequently and had larger tumors than rats consuming the wheat bran or basic diets. Parallel effects on the density of ACF were found 6 weeks after the carcinogen. Although epithelial proliferation was significantly enhanced by potato starch compared with the basic diet, the addition of wheat bran did not suppress this enhancement.
  
  
  CONCLUSIONS
  This type-2 RS enhances epithelial proliferation, ACF density, and tumor formation. The addition of wheat bran to an RS-containing diet suppresses tumorigenesis, acting on events responsible for the formation of ACF but not the events controlling the hyperproliferative phase.
authors.affiliation: Department of Medicine, University of Melbourne, Royal Melbourne Hospital, Victoria, Australia.
authors.forename: G P
authors.initials: GP
authors.lastname: Young
authors.forename: A
authors.initials: A
authors.lastname: McIntyre
authors.forename: V
authors.initials: V
authors.lastname: Albert
authors.forename: M
authors.initials: M
authors.lastname: Folino
authors.forename: J G
authors.initials: JG
authors.lastname: Muir
authors.forename: P R
authors.initials: PR
authors.lastname: Gibson
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D002273:Carcinogens / Q000633:toxicity*; D002452:Cell Count / Q000187:drug effects; D002453:Cell Cycle / Q000187:drug effects; D003106:Colon / Q000187:drug effects / Q000473:pathology; D015179:Colorectal Neoplasms / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D004043:Dietary Fiber / Q000494:pharmacology*; D004127:Dimethylhydrazines; D004195:Disease Models, Animal; D004848:Epithelium / Q000187:drug effects / Q000473:pathology; D011230:Precancerous Conditions / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D011897:Random Allocation; D051381:Rats; D012007:Rectum / Q000187:drug effects / Q000473:pathology; D011198:Solanum tuberosum; D013213:Starch / Q000633:toxicity*
chemical_list: D002273:Carcinogens; D004043:Dietary Fiber; D004127:Dimethylhydrazines; D013213:Starch