title: A male-specific nuclease-resistant chromatin fraction in the mealybug Planococcus lilacinus.
abstract: In mealybugs, chromatin condensation is related to both genomic imprinting and sex determination. The paternal chromosomal complement is condensed and genetically inactive in sons but not in daughters. During a study of chromatin organization in Planococcus lilacinus, digestion with micrococcal nuclease showed that 3% to 5% of the male genome is resistant to the enzyme. This Nuclease Resistant Chromatin (NRC) apparently has a nucleosomal organization. Southern hybridization of genomic DNA suggests that NRC sequences are present in both sexes and occur throughout the genome. Cloned NRC DNA is A+T-rich with stretches of adenines similar to those present in mouse alpha-satellite sequences. NRC DNA also contains sequence motifs that are typically associated with the nuclear matrix. Salt-fractionation experiments showed that NRC sequences are matrix associated. These observations are discussed in relation to the unusual cytological features of mealybug chromosomes, including the possible existence of multiple centres of inactivation.
authors.affiliation: Developmental Biology and Genetics Laboratory, Indian Institute of Science, Bangalore 560 012, India.
authors.forename: S
authors.initials: S
authors.lastname: Khosla
authors.forename: P
authors.initials: P
authors.lastname: Kantheti
authors.forename: V
authors.initials: V
authors.lastname: Brahmachari
authors.forename: H S
authors.initials: HS
authors.lastname: Chandra
mesh_terms: D000818:Animals; D001482:Base Composition; D001483:Base Sequence; D002463:Cell Membrane Permeability; D002467:Cell Nucleus; D002843:Chromatin / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism; D004247:DNA / Q000032:analysis* / Q000235:genetics; D005260:Female; D006570:Heterochromatin / Q000502:physiology; D006657:Histones / Q000032:analysis; D007313:Insecta / Q000737:chemistry*; D008297:Male; D008836:Micrococcal Nuclease / Q000378:metabolism; D008969:Molecular Sequence Data; D015530:Nuclear Matrix / Q000737:chemistry*; D009707:Nucleosomes; D012733:Sex Differentiation
chemical_list: D002843:Chromatin; D006570:Heterochromatin; D006657:Histones; D009707:Nucleosomes; D004247:DNA; D008836:Micrococcal Nuclease