An animal model of gastric ulcer due to bacterial gastritis in mice.
Conventional female BalbC mice were inoculated with Gastrospirillum-like bacteria in mouse gastric homogenate or in 5.0-microns filtrate of gastric homogenate. The bacteria were originally isolated from cheetahs with gastritis. The mice were killed 6 months, 7 months, or 1 year after inoculation. All mice became infected with Gastrospirillum-like bacteria that were confined to the gastric mucosa. Control mice, given either sterile Brucella broth, 0.22-microns filtrate of infected gastric homogenate, or uninfected gastric homogenate did not become infected with bacteria. Lesions in infected mice included severe lymphoplasmacytic gastritis (26/26 infected mice), gastric epithelial hyperplasia (25/26 infected mice), and gastric ulceration (11/26 infected mice). Neutrophilic inflammatory cell infiltrates were inconsistent. None of the uninfected control mice had Gastrospirillum-like bacteria, gastritis, gastric epithelial hyperplasia, or gastric ulceration. These results implicate Gastrospirillum-like bacteria from cheetahs in the pathogenesis of gastric ulceration. This model will be useful in investigating the mechanisms of gastric ulceration associated with bacterial gastritis.
Eaton
K A
KA
Department of Veterinary Pathobiology, Ohio State University, 1925 Coffey Road, Columbus, OH.
Radin
M J
MJ
Krakowka
S
S
D000818:Animals; D001424:Bacterial Infections / Q000382:microbiology* / Q000473:pathology; D005591:Chemical Fractionation; D004195:Disease Models, Animal; D005260:Female; D005753:Gastric Mucosa / Q000473:pathology / Q000648:ultrastructure; D005756:Gastritis / Q000382:microbiology* / Q000473:pathology; D016998:Helicobacter; D007150:Immunohistochemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D008854:Microscopy, Electron; D013194:Staining and Labeling; D013276:Stomach Ulcer / Q000382:microbiology* / Q000473:pathology