title: Binding of outer membrane preparations of Campylobacter jejuni to INT 457 cell membranes and extracellular matrix proteins.
abstract: Binding of outer membrane (OM) preparations of the thermophilic Campylobacter species C. jejuni to epithelial cell membranes and extracellular matrix proteins were studied in an in vitro model system using enzyme-linked immunosorbent assay. The OM preparations exhibited significant binding to INT 407 intestinal cell membranes. The process of adhesion was modulated by enzymatic, chemical or immunological pretreatment of the bacteria. Following oxidation of the lipopolysaccharide (LPS) with sodium meta-periodate, the OM preparations essentially retained their binding properties. After pretreatment with proteinase K, the OM preparations lost their binding capacity and the apparent molecular mass of the major OM protein shifted from 42 to 24 kDa. Preincubation of C. jejuni bacteria with C. jejuni-specific antiserum reduced adhesion significantly; preincubation with LPS-specific monoclonal antibodies only to a minimal extent. The OM preparations also bound significantly to the extracellular matrix proteins collagen and fibronectin; however, they bound virtually no bovine serum albumin or horse serum.
authors.affiliation: Institut für Mikrobiologie und Tierseuchen, Freie Universität Berlin, Germany.
authors.forename: I
authors.initials: I
authors.lastname: Moser
authors.forename: W
authors.initials: W
authors.lastname: Schröder
mesh_terms: D000907:Antibodies, Bacterial / Q000494:pharmacology; D001422:Bacterial Adhesion / Q000187:drug effects / Q000502:physiology*; D001798:Blood Proteins / Q000378:metabolism; D016123:Campylobacter jejuni / Q000187:drug effects / Q000276:immunology / Q000472:pathogenicity / Q000502:physiology*; D002462:Cell Membrane / Q000382:microbiology*; D002478:Cells, Cultured; D003094:Collagen / Q000378:metabolism; D003429:Cross Reactions; D019286:Endopeptidase K; D004847:Epithelial Cells; D004848:Epithelium / Q000382:microbiology; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D005353:Fibronectins / Q000378:metabolism; D006801:Humans; D007422:Intestines / Q000166:cytology / Q000382:microbiology*; D010504:Periodic Acid / Q000494:pharmacology; D011485:Protein Binding; D012697:Serine Endopeptidases / Q000494:pharmacology; D013347:Subcellular Fractions
chemical_list: D000907:Antibodies, Bacterial; D001798:Blood Proteins; D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D010504:Periodic Acid; D003094:Collagen; C009288:metaperiodate; D012697:Serine Endopeptidases; D019286:Endopeptidase K