title: Azure B-eosin APAAP staining: a method for simultaneous hematological and immunological cell analysis.
abstract: Azure B-eosin APAAP staining allows simultaneous analysis of peripheral blood and bone marrow cells for hematological characteristics and immunological cell marker profiles. A defined sequence of staining procedures maintains characteristic components of the Romanowsky-Giemsa stain whereas cell antigens can be detected immunologically using the alkaline phosphatase-anti-alkaline phosphatase (APAAP) detection system. Antigens are visualized by the staining product of the substrate-naphthol AS GR phosphate and variamine blue salt. The usefulness of the azure B-eosin APAAP method was demonstrated on blood and bone marrow smears of patients with various hematological disorders.
authors.affiliation: Department of Internal Medicine, University of Homburg, Germany.
authors.forename: B
authors.initials: B
authors.lastname: Koch
authors.forename: B
authors.initials: B
authors.lastname: Eberhardt
authors.forename: M
authors.initials: M
authors.lastname: Westerhausen
mesh_terms: D000469:Alkaline Phosphatase / Q000276:immunology*; D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000032:analysis* / Q000097:blood; D001399:Azure Stains; D015415:Biomarkers / Q000032:analysis / Q000097:blood; D001853:Bone Marrow / Q000276:immunology; D001854:Bone Marrow Cells; D004801:Eosine Yellowish-(YS); D006402:Hematologic Diseases / Q000097:blood* / Q000276:immunology; D006801:Humans; D007124:Immunoenzyme Techniques; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D001399:Azure Stains; D015415:Biomarkers; D000469:Alkaline Phosphatase; D004801:Eosine Yellowish-(YS)