Anti-hepatitis A virus antibody response elicited in mice by different forms of a synthetic VP1 peptide.
Peptide VP1 (11-25) of the capsid of hepatitis A virus was synthesized by the Fmoc-polyamide solid phase method, and administered to mice in different forms: (1) free, (2) encapsulated in multilamellar liposomes, (3) coupled to keyhole limpet hemocyanin (KHL), and (4) incorporated into a tetrameric branched lysine core. The highest anti-VP1 peptide responses were generated by synthetic peptides entrapped into liposomes and coupled to KLH. No anti-HAV response was generated with the free peptide, while all the other forms induced both anti-HAV and HAV-neutralizing antibodies. Maximum neutralization indices were observed in ascites from mice treated with liposome-entrapped and KLH peptides.
Haro
I
I
Department of Peptides, CID, CSIC, Barcelona, Spain.
Pinto
R M
RM
Gonzalez-Dankaart
J F
JF
Perez
J A
JA
Reig
F
F
Bosch
A
A
D000276:Adjuvants, Immunologic; D000595:Amino Acid Sequence; D000818:Animals; D002213:Capsid / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006433:Hemocyanins; D006506:Hepatitis A / Q000276:immunology*; D035922:Hepatitis A Antibodies; D006508:Hepatitis Antibodies / Q000032:analysis*; D006507:Hepatovirus / Q000737:chemistry / Q000276:immunology*; D007114:Immunization; D008081:Liposomes; D008239:Lysine; D051379:Mice; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000276:immunology*
D000276:Adjuvants, Immunologic; D035922:Hepatitis A Antibodies; D006508:Hepatitis Antibodies; D008081:Liposomes; D010446:Peptide Fragments; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin; D008239:Lysine