Chondrocyte-peripheral blood mononuclear cell interactions: the role of ICAM-1.
Chondrocytes express a number of cell-surface molecules that mediate cell-cell or cell-matrix interactions. The expression of intercellular adhesion molecule-1 (ICAM-1) by chondrocytes under inflammatory conditions has been well documented. Here we demonstrate the involvement of ICAM-1-, CD18- and RGD-dependent adhesion mechanisms in chondrocyte-T-cell adhesion and in cell-mediated killing of chondrocytes in vitro. In the absence of cytokine stimulation, chondrocyte-peripheral blood mononuclear cell (PBMC) interactions were unaffected by inhibition of ICAM-1-dependent pathways but were significantly reduced by blockade of CD18- and RGD-dependent pathways. Following cytokine stimulation chondrocyte-PBMC adhesion and chondrocyte killing were significantly increased. These effects could be inhibited by the blockade of ICAM-1.
Horner
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Strangeways Research Laboratory, Worts Causeway, Cambridge, UK.
Davies
M E
ME
Franz
B
B
D000818:Animals; D001170:Arthritis, Infectious / Q000276:immunology*; D018821:CD18 Antigens / Q000276:immunology; D002358:Cartilage, Articular / Q000166:cytology / Q000276:immunology*; D002448:Cell Adhesion / Q000276:immunology; D018929:Cell Culture Techniques; D003602:Cytotoxicity, Immunologic; D018799:Intercellular Adhesion Molecule-1 / Q000276:immunology* / Q000378:metabolism; D007375:Interleukin-1 / Q000276:immunology; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D009842:Oligopeptides / Q000276:immunology; D013552:Swine; D013601:T-Lymphocytes / Q000276:immunology
D018821:CD18 Antigens; D007375:Interleukin-1; D009842:Oligopeptides; D018799:Intercellular Adhesion Molecule-1; C047981:arginyl-glycyl-aspartic acid