title: Rapid and simultaneous quantification of rhamnose, mannitol, and lactulose in urine by HPLC for estimating intestinal permeability in pediatric practice.
abstract: Determination of intestinal permeability by measuring nonmetabolized sugars has been used to assess the integrity of intestinal mucosa. We have developed and validated a modified HPLC method for determining the concentration of L-rhamnose, mannitol, and lactulose in urine, using an amine-modified silica column and refractive index detection. Probe sugars are simultaneously resolved within 18 min. The calibration curve for each sugar is linear to 20 mmol/L. The minimum detectable concentration of lactulose is 0.05 mmol/L. Recovery of probe sugars is between 99.3% and 105.1%. Overall precision (CV) of estimation of probe sugars ranges from 4.2% to 6.5%. In 14 urine samples from healthy children who ingested the test solution containing 1 g of L-rhamnose, 1 g of mannitol, and 5 g of lactulose, the 5-h urinary excretion ratios of lactulose/rhamnose and lactulose/mannitol were 0.047 +/- 0.018 and 0.021 +/- 0.010 (mean +/- SD), respectively. This method presents a rapid, convenient, and practical technique for determining intestinal permeability in clinical pediatric practice.
authors.affiliation: Gastroenterology Unit, Women's and Children's Hospital, North Adelaide, Australia.
authors.forename: K
authors.initials: K
authors.lastname: Miki
authors.forename: R
authors.initials: R
authors.lastname: Butler
authors.forename: D
authors.initials: D
authors.lastname: Moore
authors.forename: G
authors.initials: G
authors.lastname: Davidson
mesh_terms: D000293:Adolescent; D002648:Child; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007422:Intestines / Q000502:physiology*; D007792:Lactulose / Q000652:urine*; D008353:Mannitol / Q000652:urine*; D010539:Permeability; D012016:Reference Values; D012210:Rhamnose / Q000652:urine*
chemical_list: D008353:Mannitol; D007792:Lactulose; D012210:Rhamnose