Detection of specific bacterial cells with 2-hydroxy-3-naphthoic acid-2'-phenylanilide phosphate and fast red TR in situ hybridization.
An in situ hybridization technique with HNPP (2-hydroxy-3-naphthoic acid-2'-phenylanilide phosphate) and Fast Red TR was used to detect specific bacterial cells at the single-cell level. By this technique, the fluorescent signals of target bacterial cells were up to eight times more intense than those of standard fluorescence in situ hybridization with mono-fluorescein isothiocyanate-labeled oligonucleotide probes. This novel HNPP-Fast Red TR whole-cell hybridization technique is available for the identification of small or low-rRNA-content bacterial cells in the natural environment.
Yamaguchi
N
N
Faculty of Pharmaceutical Sciences, Osaka University, Japan.
Inaoka
S
S
Tani
K
K
Kenzaka
T
T
Nasu
M
M
D001419:Bacteria / Q000302:isolation & purification*; D001483:Base Sequence; D004396:Coloring Agents; D003979:Diazonium Compounds; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008969:Molecular Sequence Data; D009281:Naphthalenes; D015347:RNA Probes; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013045:Species Specificity
D004396:Coloring Agents; D003979:Diazonium Compounds; D009281:Naphthalenes; D015347:RNA Probes; D012336:RNA, Ribosomal, 16S; C094602:3-hydroxy-N-(2'-biphenyl)-2-naphthalenecarboxamide phosphate; C027766:Fast Red TA