Activation of bovine photoreceptor guanylate cyclase by S100 proteins.
S100 proteins are acidic calcium-binding proteins present in high concentration in the brain and at lower concentrations in peripheral tissues. Their function remains to be elucidated in many tissues. In this report we show for the first time that S100 proteins stimulate the bovine photoreceptor membrane guanylate cyclase. The extent of stimulation varied between the different S100 proteins with the S100b exhibiting the highest level of influence. The stimulation by all S100 proteins was calcium dependent, with the half-maximal stimulation occurring at about 35-40 microM calcium. These results suggest that in some cells S100 proteins can mediate calcium signals via cyclic GMP.
Margulis
A
A
Eye Research Institute, Oakland University, Rochester, Minnesota 48309, USA.
Pozdnyakov
N
N
Sitaramayya
A
A
D000818:Animals; D002122:Calcium Chloride / Q000494:pharmacology; D002417:Cattle; D002462:Cell Membrane / Q000201:enzymology; D004789:Enzyme Activation; D006162:Guanylate Cyclase / Q000378:metabolism*; D007700:Kinetics; D012374:Rod Cell Outer Segment / Q000201:enzymology*; D009418:S100 Proteins / Q000378:metabolism* / Q000494:pharmacology
D009418:S100 Proteins; D006162:Guanylate Cyclase; D002122:Calcium Chloride