LPS-induced release of EGF, GM-CSF, GRO alpha, LIF, MIP-1 alpha and PDGF-AB in PBMC from persons with high or low levels of HDL lipoprotein.
We have examined basal and lipopolysaccharide (LPS)-induced release of epidermal growth factor (EGF), granulocyte-macrophage colony-stimulating factor (GM-CSF), growth-regulated peptide alpha (GRO alpha), leukaemia inhibitory factor (LIF), macrophage inflammatory protein-1a (MIP-1 alpha) and platelet-derived growth factor-AB (PDGF-AB) in peripheral blood mononuclear cells (PBMC) from 20 persons with either high (n = 10) or low (n = 10) levels of high-density lipoprotein (HDL). PBMC were incubated with 100 ng LPS/ml for up to 160 h, and showed a significantly higher release of the chemokines GRO alpha (P = 0.04) and MIP-1 alpha (P < 0.01) in persons with high HDL, whereas levels of GM-CSF were similar. Levels of EGF, LIF and PDGF-AB were always low, and remained unaltered during 160 h of incubation. These findings indicate that PBMC from persons with high or low levels of HDL have different functional properties, of importance in cell recruitment and activation.
Eggesbø
J B
JB
Department of Clinical Chemistry, Ullevål University Hospital, Oslo, Norway.
Hjermann
I
I
Joø
G B
GB
Ovstebø
R
R
Kierulf
P
P
D054407:Chemokine CCL4; D054360:Chemokine CXCL1; D018925:Chemokines / Q000097:blood; D019743:Chemokines, CXC; D002630:Chemotactic Factors / Q000097:blood; D016207:Cytokines / Q000097:blood*; D004815:Epidermal Growth Factor / Q000097:blood; D005260:Female; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000097:blood; D006131:Growth Inhibitors / Q000097:blood; D006133:Growth Substances / Q000097:blood; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins; D015850:Interleukin-6; D053605:Leukemia Inhibitory Factor; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008075:Lipoproteins, HDL / Q000097:blood*; D008222:Lymphokines / Q000097:blood; D019402:Macrophage Inflammatory Proteins; D008297:Male; D015846:Monokines / Q000097:blood; D009363:Neoplasm Proteins / Q000097:blood; D010982:Platelet-Derived Growth Factor / Q000378:metabolism; D013268:Stimulation, Chemical
C475829:CXCL1 protein, human; D054407:Chemokine CCL4; D054360:Chemokine CXCL1; D018925:Chemokines; D019743:Chemokines, CXC; D002630:Chemotactic Factors; D016207:Cytokines; D006131:Growth Inhibitors; D006133:Growth Substances; D036341:Intercellular Signaling Peptides and Proteins; D015850:Interleukin-6; C507837:LIF protein, human; D053605:Leukemia Inhibitory Factor; D008070:Lipopolysaccharides; D008075:Lipoproteins, HDL; D008222:Lymphokines; D019402:Macrophage Inflammatory Proteins; D015846:Monokines; D009363:Neoplasm Proteins; D010982:Platelet-Derived Growth Factor; C095477:platelet-derived growth factor AB; D004815:Epidermal Growth Factor; D016178:Granulocyte-Macrophage Colony-Stimulating Factor