Expression of an unusual Bw4 epitope by a subtype of HLA-B8 [B*0802].
The primary structure of a variant HLA-B8 antigen has been determined by cDNA cloning and sequencing. The variant, B*0802 differs, from the common B*0801 subtype at positions 77-83 of the alpha 1 helix that determine the Bw4 and Bw6 public epitopes. Whereas B*0801 has the common Bw6 motif, B*0802 has the Bw4 motif found in B*13 and B*44 allotypes. Serological analysis of B cell lines expressing B*0802 and of a B*0802 transfectant made with the HLA-A,B negative cell line 721.221 shows that B*0802 reacts with Bw4-specific antibodies, but at a level much lower than expected for Bw4 positive HLA-B allotypes.
Arnett
K L
KL
Department of Structural Biology, Stanford University, California, USA.
Adams
E J
EJ
Gumperz
J E
JE
Darke
C
C
Marsh
S G
SG
Gelsthorpe
K
K
Parham
P
P
D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology; D002461:Cell Line, Transformed; D000939:Epitopes / Q000737:chemistry / Q000276:immunology*; D005260:Female; D015235:HLA-B Antigens / Q000737:chemistry / Q000276:immunology*; D015795:HLA-B8 Antigen / Q000737:chemistry / Q000276:immunology*; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D000939:Epitopes; D015235:HLA-B Antigens; D015795:HLA-B8 Antigen; C042388:HLA-Bw4 antigen