title: PCR-fingerprinting used for comparison of ex type strains of Trichoderma species deposited in different culture collections.
abstract: PCR-fingerprinting with primers (GACA)4, (GTG)5, M13 (core sequence of phage M13), and OPB-05 was used to compare ex type strains of various species of Trichoderma. The ex type strain of each species was obtained from different culture collections. PCR-fingerprints of each ex type culture, despite of separate cultivation over a long period of time (sometimes decades) by different culture collections, were identical. Ex type strains could be discriminated from a number of other strains belonging to the same species, since every strain was characterized by its individual PCR-fingerprint. PCR-fingerprinting is recommended as a basic tool for proving the identity of strains, especially with regard to comprehensive culture collections. Investigations of Trichoderma todica, a species of undefined systematic position within the genus Trichoderma, suggest a close relationship to Trichoderma paraceramosum. Morphological and molecular data indicate that Trichoderma todica and Trichoderma parceramosum are conspecific.
authors.affiliation: Institut für Biologie (Genetik), Humboldt-Universität zu Berlin, Germany.
authors.forename: K
authors.initials: K
authors.lastname: Kuhls
authors.forename: E
authors.initials: E
authors.lastname: Lieckfeldt
authors.forename: T
authors.initials: T
authors.lastname: Börner
mesh_terms: D001483:Base Sequence; D016172:DNA Fingerprinting / Q000379:methods*; D004271:DNA, Fungal / Q000032:analysis; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods*; D013045:Species Specificity; D014242:Trichoderma / Q000145:classification / Q000235:genetics*
chemical_list: D004271:DNA, Fungal