Detection and genotyping of herpes simplex virus types 1 and 2 by polymerase chain reaction.
A simple and rapid polymerase chain reaction (PCR) procedure was developed for simultaneous detection and typing of herpes simplex virus (HSV) types 1 and 2. It was possible to detect and type HSV using two primers pairs in a simultaneous double PCR reaction, where the type of HSV present was determined on the basis of an ethidium-bromide-stained band after agarose gel electrophoresis. This PCR assay was tested on about 500 clinical specimens.
Lucotte
G
G
International Institute of Anthropology, Paris, France.
Bathelier
C
C
Lespiaux
V
V
Bali
C
C
Champenois
T
T
D001483:Base Sequence; D017931:DNA Primers; D005260:Female; D005838:Genotype; D006558:Herpes Genitalis / Q000175:diagnosis; D006561:Herpes Simplex / Q000175:diagnosis; D018259:Herpesvirus 1, Human / Q000235:genetics / Q000302:isolation & purification*; D018258:Herpesvirus 2, Human / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D012189:Retrospective Studies; D012680:Sensitivity and Specificity; D012867:Skin / Q000821:virology; D014621:Vagina / Q000821:virology
D017931:DNA Primers