Search for antibodies for immunoenzymatic assay of human lutropin.
Five peptides corresponding to human lutropin (hLH) subunit fragments were synthesized by a solid phase method and their physicochemical characteristics are presented. Antibodies induced in rabbits with 3 peptides of beta hLH fragments coupled to thyrotropin did not bind hLH and human chorionic gonadotropin (hCG). Also 2 synthetic peptides of alpha hLH fragments did not react with rabbit anti-hLH, anti-hCG and anti-alpha hCG antibodies. Using 2 monoclonal anti-alpha hCG and anti-beta hLH antibodies a sensitive sandwich ELISA technique was elaborated. Using this technique stability of hLH was investigated. The ELISA was also applied for assays of urine hLH in normal and ovulation days.
Szewczuk
A
A
Biochemical Laboratory, Polish Academy of Sciences, Wrocław.
Kuropatwa
M
M
Kochanowska
I
I
Majewska
A
A
Maćkiewicz
Z
Z
Kupryszewski
G
G
Rapak
A
A
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies; D000911:Antibodies, Monoclonal; D000917:Antibody Formation; D000918:Antibody Specificity; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004355:Drug Stability; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D007986:Luteinizing Hormone / Q000032:analysis* / Q000737:chemistry / Q000652:urine; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D010060:Ovulation; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000737:chemistry / Q000276:immunology; D011817:Rabbits; D012996:Solutions
D000906:Antibodies; D000911:Antibodies, Monoclonal; D046911:Macromolecular Substances; D010446:Peptide Fragments; D012996:Solutions; D007986:Luteinizing Hormone