title: Efficient one-step direct labelling of recombinant antibodies with technetium-99m.
abstract: High-affinity bacterially expressed antibody fragments can nowadays be cloned from established hybridomas or, more conveniently, isolated directly from antibody libraries displayed on filamentous phage. Such antibodies can be tagged with C-terminal peptide tags containing one cysteine residue, which represents a convenient functionalisation site for a number of applications, including technetium-99m labelling. Here we describe a simple one-step method for 99mTc labelling of cysteine-tagged recombinant antibodies with more than 50% radionuclide incorporation. The labelled antibodies displayed full retention of immuoreactivity and good stability.
authors.affiliation: Dipartimento di Medicina Sperimentale, Sezione di Medicina Nucleare, Policlinico Umberto I, Universita' di Roma "La Sapienza", Via Regina Elena 324, I-00161, Roma, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Liberatore
authors.forename: D
authors.initials: D
authors.lastname: Neri
authors.forename: G
authors.initials: G
authors.lastname: Neri
authors.forename: A
authors.initials: A
authors.lastname: Pini
authors.forename: A P
authors.initials: AP
authors.lastname: Iurilli
authors.forename: F
authors.initials: F
authors.lastname: Ponzo
authors.forename: G
authors.initials: G
authors.lastname: Spampinato
authors.forename: F
authors.initials: F
authors.lastname: Padula
authors.forename: A
authors.initials: A
authors.lastname: Pala
authors.forename: A C
authors.initials: AC
authors.lastname: Colella
mesh_terms: D000907:Antibodies, Bacterial; D000915:Antibody Affinity; D018796:Immunoconjugates; D007128:Immunoglobulin Fragments; D007553:Isotope Labeling / Q000379:methods*; D011994:Recombinant Proteins; D013667:Technetium
chemical_list: D000907:Antibodies, Bacterial; D018796:Immunoconjugates; D007128:Immunoglobulin Fragments; D011994:Recombinant Proteins; D013667:Technetium