title: Mitogenic effect of HIV-infected human T cell lines on mouse B cells mediated by surface immunoglobulin.
abstract: Following HIV-1 infection, a number of disorders are induced in both normal T and B cells by virus products derived from infected CD4+ T cells. In the present study, we found that HIV-infected, but not uninfected, human T cell lines generated vigorous blastogenesis and proliferation of freshly isolated mouse B cells in a short-term culture. Neither human B cells nor rat B cells showed significant responses to the HIV-infected T cell lines in the present condition. The mitogenic effect of HIV-infected human T cell line requires direct cell-cell interaction between mouse B cells and HIV-infected T cell lines. Since either mitomycin c treatment or paraformaldehyde fixation of HIV-infected T cell lines resulted in complete loss of the mitogenic effect, it seems that de novo synthesized viral products are responsible for this effect. Furthermore, anti-mouse immunoglobulin antibody inhibited completely the B cell stimulation by the HIV-infected human T cell lines. Thus, surface immunoglobulin (sIg) on mouse B cells appears to be an essential molecule which transduces activation signals from HIV-infected human T cells into cytoplasm of the B cells.
authors.affiliation: Section of Pathology, Hokkaido University, Sapporo, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Arase
authors.forename: H
authors.initials: H
authors.lastname: Arase
authors.forename: K
authors.initials: K
authors.lastname: Ohki
authors.forename: Y
authors.initials: Y
authors.lastname: Nishino
authors.forename: K
authors.initials: K
authors.lastname: Ikuta
authors.forename: K
authors.initials: K
authors.lastname: Onoe
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology / Q000821:virology*; D002450:Cell Communication / Q000187:drug effects / Q000276:immunology; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D015458:Leukemia, T-Cell; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D008810:Mice, Inbred C57BL; D008938:Mitosis / Q000187:drug effects / Q000276:immunology*; D011947:Receptors, Antigen, B-Cell / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000276:immunology / Q000821:virology*; D014407:Tumor Cells, Cultured
chemical_list: D011947:Receptors, Antigen, B-Cell