title: Endothelins inhibit cyclic AMP production in rabbit and human ciliary processes.
abstract: The effects of endothelins on cyclic AMP production have been studied in rabbit and human ciliary processes. Endothelins inhibit basal, isoproterenol-, and VIP-stimulated cyclic AMP production in rabbit ciliary processes and forskolin-stimulated cyclic AMP production in rabbit and human ciliary processes. EC50s for inhibition of forskolin-stimulated cyclic AMP production using rabbit tissue were similar: ET-1, 70 nM; ET-2, 40 nM; ET-3, 70 nM; maximum inhibition was about 76% for each ligand. These data are consistent with the presence of an ETB receptor. Endothelin-like immunoreactivity in rabbit aqueous humor was measured to be 226 pg/ml.
authors.affiliation: Department of Ophthalmology and Visual Science, Yale University School of Medicine, New Haven, Connecticut, USA.
authors.forename: L P
authors.initials: LP
authors.lastname: Bausher
mesh_terms: D000319:Adrenergic beta-Antagonists / Q000494:pharmacology; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D001082:Aqueous Humor / Q000737:chemistry; D002924:Ciliary Body / Q000187:drug effects / Q000378:metabolism*; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D016232:Endothelins / Q000032:analysis / Q000494:pharmacology*; D006801:Humans; D007545:Isoproterenol / Q000494:pharmacology; D008297:Male; D011817:Rabbits; D017466:Receptors, Endothelin / Q000378:metabolism; D014660:Vasoactive Intestinal Peptide / Q000494:pharmacology
chemical_list: D000319:Adrenergic beta-Antagonists; D016232:Endothelins; D017466:Receptors, Endothelin; D005576:Colforsin; D014660:Vasoactive Intestinal Peptide; D000242:Cyclic AMP; D007545:Isoproterenol