Human superoxide dismutase cDNA transfection and its in vitro effect on cold preservation.
In order to elucidate the role of superoxide dismutase (SOD on ischemia-reperfusion injury, bovine and porcine aortic endothelial cells were transfected with retroviral vector LSODRNL containing human Cu,Zn-SOD cDNA and their SOD activities were evaluated by measuring superoxide scavenging capability. Successful augmentation of Cu,Zn-SOD up to 2.7-fold in bovine and 1.9-fold in porcine cells was obtained. After 24 h cold preservation followed by 4 h rewarming, cell viability compared with that of pre-preservation was significantly increased in both types of cells (P < 0.01). Moreover, after exposure to superoxide, viability of the porcine cells with high SOD activity was significantly improved compared to control cells. We consider that transfection of human Cu,Zn-SOD cDNA to arterial endothelial cells is useful for preventing from cold preservation-rewarming or ischemia-reperfusion injury.
Negita
M
M
Department of Surgery II, Nagoya University School of Medicine, Japan.
Hayashi
S
S
Yokoyama
I
I
Emi
N
N
Nagasaka
T
T
Takagi
H
H
D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002470:Cell Survival; D003080:Cold Temperature; D004730:Endothelium, Vascular / Q000166:cytology / Q000201:enzymology*; D005822:Genetic Vectors; D006801:Humans; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes / Q000737:chemistry; D015427:Reperfusion Injury / Q000517:prevention & control; D012190:Retroviridae / Q000235:genetics; D013482:Superoxide Dismutase / Q000235:genetics*; D013481:Superoxides / Q000633:toxicity; D013552:Swine; D014021:Tissue Preservation; D014162:Transfection
D015345:Oligonucleotide Probes; D013481:Superoxides; D013482:Superoxide Dismutase