[On-line monitoring of nitrofurantoin in urine of rabbit by a flow-injection system with a fiber-optic chemical sensor].
A flow-injection system for monitoring of nitrofurantoin (NFT) in rabbit urine that makes use of a kind of fiber-optic chemical sensor is presented. The NFT-sensitive optrode was combined with a membrane onto which was immobilized pyrenebutyric acid (PBA) as a fluoroprobe. The NFT concentration was determined via dynamic quenching of the fluorescence of PBA. The average recovery was 96.5%-102.9%. The relative standard deviations for within-day and between-day were 3.1%-3.7% and 1.6%-3.8% respectively. The calibration curve was linear in the range from 2 micrograms.ml-1 to 100 micrograms.ml-1 with r = 0.9983. The detection limit of this method was 0.74 microgram.ml-1. The urine drug concentration-time course in rabbit after oral administration conformed to a one compartment open model with a first order absorption phase. Mean T1/2 value was 0.75 +/- 0.14 h. The selectivity was affirmed by HPTLC.
Li
W
W
Department of Pharmacy, Xinjiang Medical College, Urumqi.
Chen
J
J
D000818:Animals; D016903:Drug Monitoring / Q000295:instrumentation / Q000379:methods; D017022:Flow Injection Analysis / Q000379:methods; D008297:Male; D009582:Nitrofurantoin / Q000493:pharmacokinetics / Q000652:urine*; D011817:Rabbits
D009582:Nitrofurantoin