Cross-hybridization of the chromosome 13/21 alpha satellite DNA probe to chromosome 22 in the prenatal screening of common chromosomal aneuploidies by FISH.
In a routine application of commercially available centromeric DNA probes for the prenatal screening of common trisomies involving the autosomes 13, 18, and 21, and sex chromosomes, four cases of discrepancy between fluorescence in situ hybridization (FISH) results and follow-up cytogenetic analysis were observed from a total of 516 cases of amniocentesis. In three of these cases, the results were false negative, and in one false positive. In this case, amniocentesis was performed because of a positive triple test in a 34-year-old woman with previous infertility treatment. The alpha satellite DNA probe for chromosomes 13/21 revealed five signals in 50 per cent of uncultured amniocytes, while standard cytogenetic analysis showed a normal karyotype. FISH analysis on metaphase chromosomes demonstrated the location of the additional signal in the centromeric region of chromosome 22. This additional signal was also present in the centromeric region of chromosome 22 of the mother, providing evidence for a possible inherited polymorphism in chromosome 22 responsible for unspecific hybridization with the alpha satellite probe for chromosomes 13/21 in this case. The observed polymorphism in centromeric regions may contribute to unreliability of the use of the 13/21 alpha satellite probe for prenatal screening by FISH.
Verlinsky
Y
Y
Reproductive Genetics Institute, Chicago, Illinois, USA.
Ginsberg
N
N
Chmura
M
M
Freidine
M
M
White
M
M
Strom
C
C
Kuliev
A
A
D000328:Adult; D000649:Amniocentesis; D000653:Amniotic Fluid / Q000166:cytology; D000782:Aneuploidy; D002478:Cells, Cultured; D002904:Chromosomes, Human, 21-22 and Y; D002882:Chromosomes, Human, Pair 13; D015342:DNA Probes; D004276:DNA, Satellite / Q000235:genetics*; D003951:Diagnostic Errors; D005260:Female; D005820:Genetic Testing; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D011110:Polymorphism, Genetic; D011247:Pregnancy; D011446:Prospective Studies
D015342:DNA Probes; D004276:DNA, Satellite