title: Morphological changes in cultured human periodontal ligament cells exposed to dental materials.
abstract: The cytotoxicity of a range of dental restorative cements was assessed by continuous observation of cultures with inverted microscopy and by light microscopic study of fixed preparations, using an in vitro model with cultured test cells derived from human periodontal ligament. The sequential morphological changes observed over a seven day period showed different degrees of cell loss and patterns of injury in response to different restorative materials, reflecting primarily either nuclear or cytoplasmic damage. Attempts at recovery were frequently identified as the culture period was extended and were characterized by recolonization of denuded areas of the culture well. It was concluded that differing dental cements damage cells through a variety of mechanisms and that the test cells exhibit differing degrees of susceptibility to injury. Assays based on short-term cultures may overestimate cytotoxicity by not allowing for cell recovery from reversible injury or repopulation of monolayers by proliferation of resistant cells.
authors.affiliation: Department of Oral Pathology, University of Newcastle upon Tyne, UK.
authors.forename: A M
authors.initials: AM
authors.lastname: Adams
authors.forename: J V
authors.initials: JV
authors.lastname: Soames
authors.forename: R F
authors.initials: RF
authors.lastname: Searle
mesh_terms: D000180:Acrylic Resins / Q000009:adverse effects; D001672:Biocompatible Materials / Q000009:adverse effects*; D002478:Cells, Cultured; D003764:Dental Materials / Q000009:adverse effects*; D006801:Humans; D010513:Periodontal Ligament / Q000166:cytology* / Q000187:drug effects* / Q000293:injuries
chemical_list: D000180:Acrylic Resins; D001672:Biocompatible Materials; D003764:Dental Materials