Effect of recombinant human granulocyte colony-stimulating factor on hemodynamic and cytokine response in a porcine model of Pseudomonas sepsis.
To investigate the effect of recombinant human granulocyte colony-stimulating factor (rhG-CSF) on sepsis, chronically catheterized conscious pigs were challenged with Pseudomonas aeruginosa (8 x 10(7) colony-forming units kg-1 h-1) for 84 h (Group A, n = 8). Group B (n = 7) also received rhG-CSF at 5 micrograms kg-1 d-1, the first dose being given 30 min before starting bacterial infusion. Two of the animals in Group A died from pulmonary failure, whereas all those treated with rh-GCSF survived. Fever, severe pulmonary hypertension and systemic hypotension--the latter accompanied at first by a transient hypodynamic, and later a hyperdynamic response--were observed in all of the animals. In Group B, however, the rise in temperature, mean pulmonary arterial pressure (at a later stage of the observation), plasma levels of tumor necrosis factor, and endotoxin were significantly less than in Group A. In the rhG-CSF-treated pigs, an initial leukopenia completely recovered within 24 h (p < .05 vs. Group A). These data suggest that rhG-CSF might be beneficial in the treatment of sepsis.
Haberstroh
J
J
Chirurgische Universitätsklinik, Chirurgische Forschung, Freiburg, Germany.
Breuer
H
H
Lücke
I
I
Massarrat
K
K
Früh
R
R
Mand
U
U
Hagedorn
P
P
Brunnberg
L
L
von Specht
B U
BU
D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000627:therapeutic use*; D006439:Hemodynamics / Q000187:drug effects*; D008168:Lung / Q000187:drug effects / Q000503:physiopathology*; D008297:Male; D011552:Pseudomonas Infections / Q000188:drug therapy*; D011897:Random Allocation; D011994:Recombinant Proteins / Q000627:therapeutic use; D012129:Respiratory Function Tests; D018805:Sepsis / Q000097:blood / Q000188:drug therapy* / Q000503:physiopathology; D013552:Swine; D014409:Tumor Necrosis Factor-alpha / Q000187:drug effects*
D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D016179:Granulocyte Colony-Stimulating Factor