[Detection of minimal residual disease in acute leukemias by immunophenotyping of bone marrow cells].
Minimal residual disease (MRD) can be detected only by sophisticated methods, e.g. immunologic. The purpose of the study was to detect MRD in leukemia patients (AL) being in hematological and clinical remission (CR) by immunophenotyping of bone marrow cells and to define the role of the immunologic method in the detection of MRD. Bone marrow samples from 31 patients with ALL and AML and from 18 volunteers were taken several times. Staining of the cells was performed by double color immunofluorescence (IF) and double color immunoenzyme assay (APAAP). The detection of MRD was done by the comparison of the percent of positive cells between AL and normal cases. Each pair of the antigens (CD) was estimated in its usefulness in MRD detection. The statistical analyses were evaluated with a t-Student's test.
Deptała
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A
Kliniki Hematologii Akademii Medycznej w Warszawie.
Widzyńska
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I
Kuratowska
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Z
D000293:Adolescent; D000328:Adult; D000368:Aged; D015703:Antigens, CD / Q000032:analysis*; D014408:Biomarkers, Tumor / Q000032:analysis*; D001853:Bone Marrow / Q000737:chemistry*; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D016130:Immunophenotyping / Q000379:methods*; D008297:Male; D008875:Middle Aged; D018365:Neoplasm, Residual; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000175:diagnosis*
D015703:Antigens, CD; D014408:Biomarkers, Tumor
Wykrywanie minimalnej choroby resztkowej w ostrych białaczkach metoda immunofenotypowania komórek szpiku kostnego.