Identification of rab12 as a secretory granule-associated small GTP-binding protein in atrial myocytes.
A subfamily of small GTP-binding proteins, rab, has been shown to be involved in regulation of vesicular traffic in eukaryotic cells. The goal of this study was to identify the rab proteins associated with atrial secretory granules. A [32P]GTP-overlay assay showed the presence of multiple small GTP-binding proteins on the atrial granules. By biochemical analysis, we have demonstrated that one of the small GTP-binding proteins associated with the atrial granules is a rab12 protein (rab12p), one of the rab proteins that are most closely related to a Sec4 protein of yeast. Association of rab12p with the atrial granules was confirmed by immunogold electron microscopy. Immunoprecipitation followed by immunoblot analysis with anti-rab12 antibody showed that in addition to atria, rab12p was expressed in multiple other organs and cell lines. These results suggest that rab12p may function in vesicular traffic in multiple diverse types of cells.
Iida
H
H
Department of Anatomy, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Wang
L
L
Nishii
K
K
Ookuma
A
A
Shibata
Y
Y
D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D003594:Cytoplasmic Granules / Q000378:metabolism* / Q000648:ultrastructure; D019204:GTP-Binding Proteins / Q000032:analysis*; D006325:Heart Atria / Q000378:metabolism* / Q000648:ultrastructure; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D020691:rab GTP-Binding Proteins
D012333:RNA, Messenger; C086222:rab12 protein, Canis familiaris; D019204:GTP-Binding Proteins; D020691:rab GTP-Binding Proteins