title: Immunodiagnosis of dracunculiasis by dot-ELISA.
abstract: A dot-enzyme-linked immunosorbent assay (dot-ELISA) using soluble adult-guineaworm antigen was developed for immunodiagnosis of Dracunculus medinensis infection. The test was found to be specific and sensitive when compared with ELISA. The dot-ELISA described here was found to be time-saving and easy to perform, and its possible use as a diagnostic and epidemiological tool under field conditions is therefore discussed.
authors.affiliation: National Chemical Laboratory, Pune, India.
authors.forename: D
authors.initials: D
authors.lastname: Prakash
authors.forename: P B
authors.initials: PB
authors.lastname: Parab
authors.forename: R N
authors.initials: RN
authors.lastname: Sharma
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology; D004320:Dracunculiasis / Q000175:diagnosis*; D004321:Dracunculus Nematode / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D012698:Serologic Tests
chemical_list: D000947:Antigens, Helminth