title: Drosophila TFIIA-S is up-regulated and required during Ras-mediated photoreceptor determination.
abstract: Photoreceptor induction in the developing Drosophila eye is triggered by the activation of the Ras pathway. Subsequently, the Ras-mediated activation of site-specific transcription factors leads to the expression of putative "effector" genes. The coactivator function of the basal transcription factor TFIIA has been shown previously to enhance the trans-activation potential of site-specific transcription factors in vitro. Here, we show that the expression of the small subunit of TFIIA (dTFIIA-S) is specifically up-regulated in a transient manner during Ras-mediated photoreceptor induction. Furthermore, although null mutations in dTFIIA-S are cell lethal, a hypomorphic dTFIIA-S allele demonstrates an increased requirement for this factor during photoreceptor development. In addition, the cone cell to R7 photoreceptor transformation caused by ectopic activation of the Ras pathway during eye development is suppressed by the removal of one functional copy of the dTFIIA-S locus revealing the sensitivity of this process to reductions in dTFIIA-S activity. These results are the first in vivo evidence for the coactivator function in transcriptional enhancement proposed for TFIIA.
authors.affiliation: Differentiation Programme, European Molecular Biology Laboratory, Heidelberg, Germany.
authors.forename: M P
authors.initials: MP
authors.lastname: Zeidler
authors.forename: K
authors.initials: K
authors.lastname: Yokomori
authors.forename: R
authors.initials: R
authors.lastname: Tjian
authors.forename: M
authors.initials: M
authors.lastname: Mlodzik
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000235:genetics; D003001:Cloning, Molecular; D004330:Drosophila / Q000235:genetics / Q000254:growth & development*; D029721:Drosophila Proteins; D051959:ELAV Proteins; D004627:Embryonic Induction / Q000235:genetics; D005314:Embryonic and Fetal Development / Q000235:genetics; D005123:Eye / Q000254:growth & development; D005136:Eye Proteins / Q000235:genetics; D018628:Gene Dosage; D018507:Gene Expression Regulation, Developmental; D011905:Genes, ras; D006720:Homozygote; D007106:Immune Sera; D008562:Membrane Glycoproteins / Q000235:genetics; D009154:Mutation; D009799:Ocular Physiological Phenomena; D017956:Photoreceptor Cells, Invertebrate / Q000254:growth & development / Q000378:metabolism / Q000502:physiology*; D020794:Receptor Protein-Tyrosine Kinases; D017949:Retinal Cone Photoreceptor Cells / Q000502:physiology; D012261:Ribonucleoproteins / Q000096:biosynthesis / Q000235:genetics; D035561:Transcription Factor TFIIA; D014157:Transcription Factors / Q000032:analysis / Q000235:genetics*; D014158:Transcription, Genetic; D015854:Up-Regulation
chemical_list: D029721:Drosophila Proteins; D051959:ELAV Proteins; D005136:Eye Proteins; D007106:Immune Sera; D008562:Membrane Glycoproteins; D012261:Ribonucleoproteins; D035561:Transcription Factor TFIIA; D014157:Transcription Factors; D020794:Receptor Protein-Tyrosine Kinases; C056737:sev protein, Drosophila