Cometabolic degradation of o-cresol and 2,6-dimethylphenol by Penicillium frequentans Bi 7/2.
o-Cresol induced glucose-grown resting mycelia of Penicillium frequentans Bi 7/2 (ATCC-number: 96048) immediately oxidized o-cresol and other phenols. After precultivation on glucose and phenol degradation started after a lag-phase of 24 hours. Metabolites of o-cresol metabolism were methylhydroquinone, methyl-p-benzoquinone, 2-methyl-5-hydroxyhydroquinone and 2-methyl-5-hydroxy-p-benzoquinone. The initial reaction is probably catalyzed by a NADPH dependent hydroxylase which is specific for o-cresol. The metabolism of 2,6-dimethylphenol (2,6-xylenol) occurred via 2,6-dimethylhydroquinone, 2,6-dimethyl-p-benzoquinone, 2,6-dimethyl-3-hydroxyhydroquinone, 2,6-dimethyl-3-hydroxy-p-benzoquinone and 3-methyl-2-hydroxybenzoic acid.
Hofrichter
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Friedrich-Schiller-Universität Jena, Institut für Mikrobiologie, Germany.
Bublitz
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Fritsche
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D002851:Chromatography, High Pressure Liquid; D003408:Cresols / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry; D006900:Hydroxylation; D007700:Kinetics; D013058:Mass Spectrometry; D006899:Mixed Function Oxygenases / Q000378:metabolism; D008956:Models, Chemical; D015394:Molecular Structure; D010407:Penicillium / Q000378:metabolism*; D010636:Phenols / Q000378:metabolism*; D011809:Quinones / Q000378:metabolism; D013053:Spectrophotometry; D014992:Xylenes / Q000378:metabolism*
D003408:Cresols; D003470:Culture Media; D010636:Phenols; D011809:Quinones; D014992:Xylenes; D006899:Mixed Function Oxygenases; C036531:2,6-xylenol