Analysis of Wnt/Engrailed signaling in Xenopus embryos using biolistics.
We adapted the Biolistics Particle Delivery System for the introduction of DNA into Xenopus embryos, allowing us to modulate the expression of different genes at specific time points during development. In the present study we applied the Biolistics method to the study of the wnt-engrailed signaling cascade in the developing Xenopus embryo. We show that ectopic expression of Xwnt-1 and Xwnt-5C is sufficient to activate specifically XEn-1 and not XEn-2.
Koster
J G
JG
Hubrecht Laboratory, Netherlands Institute for Developmental Biology, Utrecht, The Netherlands.
Eizema
K
K
Peterson-Maduro
L J
LJ
Stegeman
B I
BI
Destrée
O H
OH
D000818:Animals; D018507:Gene Expression Regulation, Developmental; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D014018:Tissue Distribution; D014162:Transfection / Q000379:methods; D014981:Xenopus / Q000196:embryology; D029867:Xenopus Proteins; D000099266:Engrailed 2 Protein
D018398:Homeodomain Proteins; D009419:Nerve Tissue Proteins; D011506:Proteins; D029867:Xenopus Proteins; D000099266:Engrailed 2 Protein; C081542:En1 protein, Xenopus