Effect of antidenaturant drugs on lysozyme deposit formation on soft contact lenses by liquid chromatography--electrochemical detection.
The effect of some antidenaturant drugs on the formation of lysozyme deposits on soft contact lenses was investigated by in vitro experiments. Results were obtained which encourage the use of this strategy to reduce lens spoilage. At the same time emphasis is given to the possibility that some functionalities (i.e. lysine groups) present in drug molecules could promote protein adsorption. In order to evaluate at a quantitative level the effectiveness of the drugs used to minimize the formation of deposits on soft contact lenses, a selective method for the determination of adsorbed lysozyme was developed. It is based on the hydrolysis of the 'lens/adsorbed lysozyme' system, followed by high performance liquid chromatography coupled to electrochemical detection of the released tryptophan residues. The lysozyme calibration curve was linear up to 5 mg ml-1 and the detection limit was 5 ng injected.
Guerrieri
A
A
Dipartimento di Chimica, Università della Basilicata, Potenza, Italy.
Sabbatini
L
L
Zambonin
P G
PG
Ricci
F
F
Pocobelli
A
A
Cerulli
L
L
D000327:Adsorption; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002853:Chromatography, Liquid; D003263:Contact Lenses, Hydrophilic; D004563:Electrochemistry / Q000379:methods; D006868:Hydrolysis; D007700:Kinetics; D009113:Muramidase / Q000032:analysis / Q000737:chemistry*; D011489:Protein Denaturation / Q000187:drug effects*; D014364:Tryptophan / Q000032:analysis
D014364:Tryptophan; D009113:Muramidase