title: Evidence against a role for interleukin-10 in the regulation of growth of Mycobacterium avium in human monocytes.
abstract: Interleukin-10 (IL-10) inhibits intracellular Mycobacterium avium killing by cytokine-activated murine macrophages and may have a role in pathogenesis. Cytokine activities in supernatants of M. avium-infected human monocytes were maximal at 6-24 h for tumor necrosis factor (TNF)-alpha and 24-48 h for IL-10. TNF-alpha and IL-10 production increased with increasing M. avium-to-monocyte infection ratios (20:1 to 200:1). TNF-alpha production by monocytes infected with smooth, domed, and opaque organisms at 200:1 exceeded that of monocytes infected with smooth, flat, and transparent M. avium (P < .01). IL-10 induction demonstrated considerable strain-to-strain variability and did not correlate with intracellular M. avium growth. IL-10 significantly inhibited TNF-alpha, IL-1 beta, and IL-6 production by M. avium-infected monocytes. Coculturing monocytes with IL-10 after M. avium infection did not affect intracellular M. avium growth. Differential induction of TNF-alpha may be a factor in the intracellular growth of M. avium in human monocytes. IL-10, however, played no apparent role in pathogenicity in this model.
authors.affiliation: Department of Medicine, Case Western Reserve University, University Hospitals of Cleveland, Ohio 44106-4984, USA.
authors.forename: H
authors.initials: H
authors.lastname: Shiratsuchi
authors.forename: B
authors.initials: B
authors.lastname: Hamilton
authors.forename: Z
authors.initials: Z
authors.lastname: Toossi
authors.forename: J J
authors.initials: JJ
authors.lastname: Ellner
mesh_terms: D001483:Base Sequence; D002478:Cells, Cultured; D015169:Colony Count, Microbial; D003470:Culture Media; D017931:DNA Primers / Q000737:chemistry; D015342:DNA Probes / Q000737:chemistry; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007375:Interleukin-1 / Q000096:biosynthesis; D016753:Interleukin-10 / Q000096:biosynthesis / Q000494:pharmacology / Q000502:physiology*; D015850:Interleukin-6 / Q000096:biosynthesis; D008969:Molecular Sequence Data; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism / Q000382:microbiology*; D015269:Mycobacterium avium Complex / Q000254:growth & development*; D012333:RNA, Messenger / Q000096:biosynthesis; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis; D015854:Up-Regulation
chemical_list: D003470:Culture Media; D017931:DNA Primers; D015342:DNA Probes; D007375:Interleukin-1; D015850:Interleukin-6; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10