title: The glycosylation of red cell autoantibodies affects their functional activity in vitro.
abstract: Factors governing the functional activity of red cell autoantibodies are poorly defined. Here we report the presence of qualitative differences in the glycosylation of IgG autoantibodies which affect in vitro interactions with Fc gamma RIII. The following antibodies were affinity-purified by adsorption and elution from normal red cells: IgG eluted from the red cells of 27 haemolysing or non-haemolysing patients, anti-D in sera from 11 pregnant women, and IgG1 and IgG3 human monoclonal anti-D. Monoclonal antibodies with differing levels of agalactosyl IgG were produced by culturing cell lines at high or low cell density. The % IgG with oligosaccharides lacking terminal galactose residues (agalactosyl IgG) of antibodies was designated as low, medium or high according to their reactivity with a monoclonal antibody to terminal N-acetylglucosamine. Fc gamma RIII-mediated functional activity was assessed by measuring the K-cell-mediated lysis of red cells in eluates diluted to achieve comparable levels of red cells sensitization. All eluates containing allo-anti-D were lytic (range 74-100%). In contrast, lysis by autoantibodies varied from 0 to 100%; 11/13 eluates from red cells of haemolysing patients promoted > 5% lysis compared to 2/7 eluates from red cells of non-haemolysing patients (P < 0.02). The ability of autoantibodies to promote K-cell-mediated red cell lysis correlated inversely with their level of agalactosyl IgG (r = -0.56, P < 0.01, n = 23). Further, monoclonal anti-D antibodies with very low levels of agalactosyl IgG were comparatively more lytic than the same antibodies containing more agalactosyl IgG. Analysis of the ratio of kappa:lambda light chains suggested that autoantibodies from 6/19 patients were monoclonal or oligoclonal in nature. The data indicate that IgG red cell autoantibodies from different patients are functionally heterogenous, and that this may be due, at least in part, to qualitative differences in the Fc region glycosylation reflected by differences in the proportion of agalactosyl IgG. This heterogeneity is consistent with the clonally-restricted nature of the autoantibodies in some patients.
authors.affiliation: International Blood Group Reference Laboratory, Bristol, U.K.
authors.forename: A G
authors.initials: AG
authors.lastname: Hadley
authors.forename: B
authors.initials: B
authors.lastname: Zupanska
authors.forename: B M
authors.initials: BM
authors.lastname: Kumpel
authors.forename: C
authors.initials: C
authors.lastname: Pilkington
authors.forename: H L
authors.initials: HL
authors.lastname: Griffiths
authors.forename: K A
authors.initials: KA
authors.lastname: Leader
authors.forename: J
authors.initials: J
authors.lastname: Jones
authors.forename: D J
authors.initials: DJ
authors.lastname: Booker
authors.forename: R
authors.initials: R
authors.lastname: Stamps
authors.forename: R J
authors.initials: RJ
authors.lastname: Sokol
mesh_terms: D001323:Autoantibodies / Q000502:physiology*; D004912:Erythrocytes / Q000276:immunology* / Q000378:metabolism; D006031:Glycosylation; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism; D007147:Immunoglobulin Light Chains / Q000378:metabolism; D007145:Immunoglobulin kappa-Chains / Q000378:metabolism; D007694:Killer Cells, Natural / Q000378:metabolism; D017452:Receptors, IgG / Q000502:physiology
chemical_list: D001323:Autoantibodies; D007074:Immunoglobulin G; D007147:Immunoglobulin Light Chains; D007145:Immunoglobulin kappa-Chains; D017452:Receptors, IgG