Immunolocalization of transforming growth factor-beta 1 and transforming growth factor-beta 2 in the mouse ovary during gonadotrophin-induced follicular maturation.
An immunohistochemical approach was utilized to evaluate the cellular distribution of transforming growth factor-beta 1 (TGF beta 1) and transforming growth factor beta 2 (TGF beta 2) at different stages of follicle development in the prepubertal mouse ovary under the following conditions: (i) after pregnant mare's serum gonadotrophin (PMSG) treatment; (ii) after PMSG and human chorionic gonadotrophin (HCG) treatment; (iii) after PMSG and HCG treatment plus mating. In the immature ovary, TGF beta 1 and TGF beta 2 immunoreactivities are localized in theca and granulosa cells and in oocytes. After PMSG treatment, TGF beta 1 and TGF beta 2 immunoreactivities are localized in granulosa cells; in addition, TGF beta 2 staining is noted in the matrix surrounding antral cells. Staining for both TGR beta 1 and TGF beta 2 drops in the theca but persists in the oocyte. PMSG plus HCG treatment results in a significant increase in TGF beta 1 and TGF beta 2 immunoreactivity in the theca and in the maintenance of TGF beta 1 staining in both basal granulosa cells and cumulus cells whereas TGF beta 2 immunoreactivity is essentially localized in the matrix surrounding cumulus cells. Staining for TGF beta 1 and TGF beta 2 persists in the oocyte. Following PMSG plus HCG treatment and mating, TGF beta 1 immunoreactivity is localized in the luteal cells of corpora lutea and TGF beta 2 shows a similar localization pattern. This study provides evidence that TGF beta 1 and TGF beta 2 peptides are expressed in specific cell types during induced follicular maturation in the mouse ovary.
Ghiglieri
C
C
INSERM U407, Centre Hospitalier Lyon-Sud, Pierre-Bénite, France.
Khatchadourian
C
C
Tabone
E
E
Hendrick
J C
JC
Benahmed
M
M
Ménézo
Y
Y
D000818:Animals; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D004359:Drug Therapy, Combination; D005260:Female; D005498:Follicular Phase / Q000378:metabolism*; D006064:Gonadotropins, Equine / Q000494:pharmacology*; D006801:Humans; D007150:Immunohistochemistry; D051379:Mice; D006080:Ovarian Follicle / Q000187:drug effects* / Q000254:growth & development; D010053:Ovary / Q000737:chemistry / Q000187:drug effects*; D012726:Sexual Behavior, Animal / Q000502:physiology; D012741:Sexual Maturation / Q000502:physiology*; D016212:Transforming Growth Factor beta / Q000032:analysis*
D006063:Chorionic Gonadotropin; D006064:Gonadotropins, Equine; D016212:Transforming Growth Factor beta