Whole-cell analysis of NGK2 (mKv3.1a) K+ channels stably expressed in mouse fibroblast cells.
NGK2 (mKv3.1a) K+ channel cDNA was introduced into mouse B82 fibroblast cells to express in a mammalian system. The NGK2 current in the stably transformed fibroblast cells exhibited a high threshold for activation and slow decay with two components. The data suggest that the NGK2 channel may contribute to slowly inactivating K+ currents observed in excitable and inexcitable cells.
Kawamura
T
T
Department of Biophysics, Kanazawa University School of Medicine, Japan.
Enomoto
K
K
Yokoyama
S
S
Hoshi
N
N
Yamashita
J
J
Higashida
H
H
D000818:Animals; D002999:Clone Cells; D018076:DNA, Complementary / Q000235:genetics; D005347:Fibroblasts / Q000378:metabolism; D008564:Membrane Potentials / Q000502:physiology; D051379:Mice; D008809:Mice, Inbred C3H; D018408:Patch-Clamp Techniques; D010957:Plasmids; D015221:Potassium Channels / Q000032:analysis* / Q000235:genetics; D014162:Transfection; D014170:Transformation, Genetic
D018076:DNA, Complementary; D015221:Potassium Channels