Evidence for an increased intracellular free calcium concentration in platelets of bronchial asthma patients.
The pathogenesis of bronchial asthma is not yet fully understood. Recently much attention has been given to the hypothesis that intracellular free calcium ([Ca2+]i) metabolism is abnormal in various diseases. In this study we investigated whether [Ca2+]i exists abnormally in subjects with bronchial asthma. The [Ca2+]i in 32 treated or untreated subjects with bronchial asthma were compared with 63 normal subjects. Resting levels of [Ca2+]i were estimated by loading the fluorescent indicator Fura-2 in washed platelets. The [Ca2+]i level in the control subjects was 129.7 +/- 18.0 nM (mean +/- SD). However, in that of the bronchial asthma patients was 152.7 +/- 44.1 nM, significantly higher than that of the control subjects (p < 0.05). It is well recognized that an increase of [Ca2+]i in vascular smooth muscle involves contraction. The findings suggest that the same phenomenon is quite possible in the tracheal smooth muscle and that it plays an important role in the pathogenesis of bronchial asthma.
Kuroda
S
S
Department of Clinical Research, National Okura Hospital, Tokyo.
Ishikawa
K
K
Hanamitsu
H
H
Komori
M
M
Komiya
K
K
Ichikawa
Y
Y
Maejima
K
K
Hasegawa
K
K
Ninomiya
R
R
Kuroda
M
M
D000328:Adult; D001249:Asthma / Q000097:blood* / Q000209:etiology; D001792:Blood Platelets / Q000378:metabolism* / Q000473:pathology; D002118:Calcium / Q000097:blood*; D005260:Female; D006801:Humans; D007424:Intracellular Fluid / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D013050:Spectrometry, Fluorescence
D002118:Calcium