title: Hsp90 is required for the activity of a hepatitis B virus reverse transcriptase.
abstract: The heat shock protein Hsp90 is known as an essential component of several signal transduction pathways and has now been identified as an essential host factor for hepatitis B virus replication. Hsp90 interacts with the viral reverse transcriptase to facilitate the formation of a ribonucleoprotein (RNP) complex between the polymerase and an RNA ligand. This RNP complex is required early in replication for viral assembly and initiation of DNA synthesis through a protein-priming mechanism. These results thus invoke a role for the Hsp90 pathway in the formation of an RNP.
authors.affiliation: Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, PA 19111, USA.
authors.forename: J
authors.initials: J
authors.lastname: Hu
authors.forename: C
authors.initials: C
authors.lastname: Seeger
mesh_terms: D000818:Animals; D016227:Benzoquinones; D006528:Carcinoma, Hepatocellular; D002460:Cell Line; D002645:Chickens; D004261:DNA Replication; D004791:Enzyme Inhibitors / Q000494:pharmacology; D018841:HSP90 Heat-Shock Proteins / Q000302:isolation & purification / Q000378:metabolism*; D006515:Hepatitis B virus / Q000201:enzymology* / Q000502:physiology; D007700:Kinetics; D047029:Lactams, Macrocyclic; D008113:Liver Neoplasms; D008957:Models, Genetic; D011809:Quinones / Q000494:pharmacology; D012367:RNA, Viral / Q000378:metabolism*; D012194:RNA-Directed DNA Polymerase / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism; D012261:Ribonucleoproteins / Q000302:isolation & purification / Q000378:metabolism; D015398:Signal Transduction; D014162:Transfection; D014407:Tumor Cells, Cultured; D014779:Virus Replication
chemical_list: D016227:Benzoquinones; D004791:Enzyme Inhibitors; D018841:HSP90 Heat-Shock Proteins; D047029:Lactams, Macrocyclic; D011809:Quinones; D012367:RNA, Viral; D011994:Recombinant Proteins; D012261:Ribonucleoproteins; D012194:RNA-Directed DNA Polymerase; C001277:geldanamycin