title: Characterization and chromosomal location of two repeated DNAs in three Gerbillus species.
abstract: Two tandemly repeated DNA sequences of Gerbillus nigeriae (Rodentia) (GN1 and GN2) were isolated and characterized. Both share a 36bp repeated unit, which includes a 20bp motif also found in primate alphoid and other repeated DNAs. The localization of GN1 and GN2 sequences on metaphase chromosomes of three Gerbillus species, G. nigeriae, G. aureus and G. nanus, was studied by fluorescence in situ hybridization (FISH). In the G. nigeriae and G. aureus karyotypes, which were shown to possess large amounts of heterochromatin and to have undergone multiple rearrangements during evolution, both GN1 and GN2 sequences were observed at various chromosomal sites: centromeric, telomeric and intercalary. In contrast, the karyotypically stable G. nanus, which does not possess large amounts of heterochromatin and seems to be a more ancestral species, possesses only GN1 sequences, localized in the juxtacentromeric regions.
authors.affiliation: Institut Curie-CNRS UMR 147, 26, rue d Ulm, F-75231 Paris Cedex 05, France.
authors.forename: V
authors.initials: V
authors.lastname: Volobouev
authors.forename: N
authors.initials: N
authors.lastname: Vogt
authors.forename: E
authors.initials: E
authors.lastname: Viegas-Péquignot
authors.forename: B
authors.initials: B
authors.lastname: Malfoy
authors.forename: B
authors.initials: B
authors.lastname: Dutrillaux
mesh_terms: D000818:Animals; D001483:Base Sequence; D005075:Biological Evolution; D015139:Blotting, Southern; D002478:Cells, Cultured; D002871:Chromosome Banding; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D005849:Gerbillinae / Q000145:classification / Q000235:genetics*; D006570:Heterochromatin; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D008969:Molecular Sequence Data; D010802:Phylogeny; D012091:Repetitive Sequences, Nucleic Acid; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D006570:Heterochromatin