Overexpression of extracellular sucrase (SacC) of Zymomonas mobilis in Escherichia coli.
The extracellular sucrase (SacC) gene of Zymomonas mobilis was overexpressed in Escherichia coli BL21 using the T7 polymerase expression system. A low cell density induction method was designed to have maximum expression, and the conditions (IPTG concentration, ampicillin addition) were optimised to overexpress to the level of more than 60% of the total cellular protein representing SacC protein.
Kannan
R
R
Department of Microbial Technology, School of Biological Sciences, Madurai Kamaraj University, India.
Pitchaimani
K
K
Gunasekaran
P
P
Aït-Abdelkader
N
N
Baratti
J
J
D001431:Bacteriological Techniques; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D015870:Gene Expression / Q000235:genetics; D005798:Genes, Bacterial / Q000235:genetics*; D013393:Sucrase / Q000032:analysis / Q000235:genetics*; D014764:Viral Proteins; D017355:Zymomonas / Q000201:enzymology / Q000235:genetics*
D014764:Viral Proteins; C062210:bacteriophage T7 RNA polymerase; D012321:DNA-Directed RNA Polymerases; D013393:Sucrase