In vivo damage to chorioallantoic membrane blood vessels by porphycene-induced photodynamic therapy.
Photodynamic therapy (PDT) was performed in the chick embryo chorioallantoic membrane (CAM) for the purpose of quantitative evaluation of several porphycenes as potential photosensitizers. Porphycenes are structural isomers of porphine possessing lower symmetry of the macrocycle and are characterized by 10-fold higher absorption at the therapeutic wavelengths for PDT (lambda > 630 nm). PDT-induced damage to CAM blood vessels included vasoconstriction and blanching as was monitored during irradiation and videotaped. Image analysis techniques enabled us to follow PDT-induced constriction of vessel diameter (to 50%), reduction of blood perfusion (to 40% lower optical density) and shrinkage of implanted tumours (to 10% of their original area). The observed PDT efficacy of functionalized porphycenes is positively correlated with the number of polar substituents.
Gottfried
V
V
Department of Chemistry, Technion-Israel Institute of Technology, Haifa, Israel.
Davidi
R
R
Averguj
C
C
Kimel
S
S
D016475:3T3 Cells; D000482:Allantois / Q000098:blood supply / Q000187:drug effects* / Q000528:radiation effects; D000818:Animals; D001808:Blood Vessels / Q000187:drug effects* / Q000528:radiation effects; D002455:Cell Division; D002642:Chick Embryo; D005321:Extraembryonic Membranes / Q000098:blood supply / Q000187:drug effects* / Q000528:radiation effects; D007834:Lasers; D051379:Mice; D009336:Necrosis; D009374:Neoplasms, Experimental / Q000209:etiology; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000494:pharmacology*; D011166:Porphyrins / Q000494:pharmacology*; D051381:Rats; D014407:Tumor Cells, Cultured; D014661:Vasoconstriction
D017319:Photosensitizing Agents; D011166:Porphyrins; C051407:porphycene