title: A common region of loss of heterozygosity in Wilms' tumor and embryonal rhabdomyosarcoma distal to the D11S988 locus on chromosome 11p15.5.
abstract: The development of Wilms' tumor has been associated with two genetic loci on chromosome 11: WT1 in 11p13 and WT2 in 11p15.5. Here, we have used loss of heterozygosity (LOH) in Wilms' tumors to narrow the WT2 locus distal to the D11S988 locus. A similar region was apparent for the clinically associated tumor, embryonal rhabdomyosarcoma. We have also demonstrated that a constitutional chromosome translocation breakpoint associated with Beckwith-Wiedemann syndrome and an acquired somatic chromosome translocation breakpoint in a rhabdoid tumor each occur in the same chromosomal interval as the smallest region of LOH in Wilms' tumors and embryonal rhabdomyosarcoma. Finally, we report the first Wilms' tumor without a cytogenetic deletion that shows targeted LOH for 11p15 and 11p13 while maintaining germline status for 11p14.
authors.affiliation: Ludwig Institute for Cancer Research, University of California San-Diego, School of Medicine, La Jolla 92093-0660, USA.
authors.forename: C
authors.initials: C
authors.lastname: Besnard-Guérin
authors.forename: I
authors.initials: I
authors.lastname: Newsham
authors.forename: R
authors.initials: R
authors.lastname: Winqvist
authors.forename: W K
authors.initials: WK
authors.lastname: Cavenee
mesh_terms: D001483:Base Sequence; D001506:Beckwith-Wiedemann Syndrome / Q000235:genetics; D002460:Cell Line; D002648:Child; D002880:Chromosomes, Human, Pair 11; D017353:Gene Deletion; D016162:Genes, Wilms Tumor / Q000235:genetics*; D005819:Genetic Markers; D006579:Heterozygote; D006801:Humans; D006822:Hybrid Cells; D007680:Kidney Neoplasms / Q000235:genetics*; D008969:Molecular Sequence Data; D018233:Rhabdomyosarcoma, Embryonal / Q000235:genetics*; D014178:Translocation, Genetic; D009396:Wilms Tumor / Q000235:genetics*
chemical_list: D005819:Genetic Markers