Comparison of reversed-phase liquid chromatography with colorimetry for analysis of phenolphthalein preparations.
The high-performance liquid chromatography (HPLC) method of the United States Pharmacopeia has been compared with the colorimetric method of the British Pharmacopoeia for the assay of phenolphthalein in various preparations. Results are presented for the linearity, sensitivity and reproducibility of the two methods. The HPLC method was considered to be more convenient for routine analysis of the preparations of phenolphthalein.
Torrado
S
S
Department of Pharmaceutical Technology, Faculty of Pharmacy, Complutense University, Madrid, Spain.
Fraile
S
S
Torrado
J J
JJ
Selles
E
E
D002138:Calibration; D002400:Cathartics / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004364:Pharmaceutical Preparations / Q000032:analysis; D020113:Phenolphthalein; D010635:Phenolphthaleins / Q000032:analysis*; D012015:Reference Standards; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*
D002400:Cathartics; D004364:Pharmaceutical Preparations; D010635:Phenolphthaleins; D020113:Phenolphthalein