title: Human papillomavirus 11 E5a delays the growth restriction induced by temperature shift in temperature-sensitive simian virus 40 T antigen-immortalized keratinocytes.
abstract: The transfection with human papillomavirus type 11 E5a oncoprotein can cause longer and more active proliferation in the human keratinocytes previously transfected stably with temperature sensitive SV40 T antigen at 39 degrees C. Also, after the E5a transfection in parental temperature sensitive SV40 T antigen gene transfected cells, we observe a delay in the accumulation of p21 gene at 39 degrees C. Moreover, T antigen degradation did not occur at 39 degrees C in the E5a transfected cells as it did in the parental cells. We draw from these observations that E5a may transiently stabilize temperature sensitive T antigen and/or repress p21 synthesis.
authors.affiliation: Dept. of Microbiology and Immunology, National Defense Medical Center, Taipei, Taiwan, Republic of China.
authors.forename: Y P
authors.initials: YP
authors.lastname: Tsao
authors.forename: S W
authors.initials: SW
authors.lastname: Kuo
authors.forename: S F
authors.initials: SF
authors.lastname: Li
authors.forename: T C
authors.initials: TC
authors.lastname: Tsai
authors.forename: L Y
authors.initials: LY
authors.lastname: Li
authors.forename: S L
authors.initials: SL
authors.lastname: Chen
mesh_terms: D000952:Antigens, Polyomavirus Transforming / Q000235:genetics*; D002455:Cell Division; D002460:Cell Line; D015967:Gene Expression Regulation, Viral; D005816:Genetic Complementation Test; D006801:Humans; D015603:Keratinocytes / Q000166:cytology; D009856:Oncogene Proteins, Viral / Q000235:genetics*; D009857:Oncogenes; D027383:Papillomaviridae / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D014162:Transfection
chemical_list: D000952:Antigens, Polyomavirus Transforming; C081917:E5A protein, Human papillomavirus type 11; D009856:Oncogene Proteins, Viral; D012333:RNA, Messenger