Gene structure, DNA methylation, and imprinted expression of the human SNRPN gene.
The human SNRPN (small nuclear ribonucleoprotein polypeptide N) gene is one of a gene family that encode proteins involved in pre-mRNA splicing and maps to the smallest deletion region involved in the Prader-Willi syndrome (PWS) within chromosome 15q11-q13. Paternal only expression of SNRPN has previously been demonstrated by use of cell lines from PWS patients (maternal allele only) and Angelman syndrome (AS) patients (paternal allele only). We have characterized two previously unidentified 5' exons of the SNRPN gene and demonstrate that exons -1 and 0 are included in the full-length transcript. This gene is expressed in a wide range of somatic tissues and at high, approximately equal levels in all regions of the brain. Both the first exon of SNRPN (exon -1) and the putative transcription start site are embedded within a CpG island. This CpG island is extensively methylated on the repressed maternal allele and is unmethylated on the expressed paternal allele, in a wide range of fetal and adult somatic cells. This provides a quick and highly reliable diagnostic assay for PWS and AS, which is based on DNA-methylation analysis that has been tested on > 100 patients in a variety of tissues. Conversely, several CpG sites approximately 22 kb downstream of the transcription start site in intron 5 are preferentially methylated on the expressed paternal allele in somatic tissues and male germ cells, whereas these same sites are unmethylated in fetal oocytes. These findings are consistent with a key role for DNA methylation in the imprinted inheritance and subsequent gene expression of the human SNRPN gene.
Glenn
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CC
R. C. Philips Research and Education Unit, Department of Pediatrics, University of Florida College of Medicine, Gainesville 32610, USA.
Saitoh
S
S
Jong
M T
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Filbrandt
M M
MM
Surti
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U
Driscoll
D J
DJ
Nicholls
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RD
D000293:Adolescent; D000328:Adult; D000595:Amino Acid Sequence; D017204:Angelman Syndrome / Q000235:genetics* / Q000378:metabolism; D001324:Autoantigens / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D002648:Child; D002675:Child, Preschool; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D018387:Codon, Initiator / Q000235:genetics; D018899:CpG Islands; D004247:DNA / Q000235:genetics / Q000378:metabolism; D005091:Exons / Q000235:genetics; D005260:Female; D018392:Genomic Imprinting / Q000235:genetics*; D006801:Humans; D007223:Infant; D008297:Male; D008745:Methylation; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011218:Prader-Willi Syndrome / Q000235:genetics* / Q000378:metabolism; D012326:RNA Splicing; D017411:Ribonucleoproteins, Small Nuclear / Q000737:chemistry / Q000235:genetics*; D055517:snRNP Core Proteins
D001324:Autoantigens; D018387:Codon, Initiator; D017411:Ribonucleoproteins, Small Nuclear; C526417:SNRPN protein, human; D055517:snRNP Core Proteins; D004247:DNA