title: Biodegradation of the nitroaromatic herbicide dinoseb (2-sec-butyl-4,6-dinitrophenol) under reducing conditions.
abstract: The degradation pathway for dinoseb (2-sec-butyl-4,6-dinitrophenol) under reducing conditions was investigated. Cultures were inoculated with a dinoseb-degrading anaerobic enrichment culture used in field studies. Biotransformation intermediates were extracted with ethyl acetate and analyzed by high pressure liquid chromatography, gas chromatography, and mass spectrometry. Dinoseb degradation involves reduction of the nitro groups to amino groups followed by replacement with hydroxyl groups. Depending on the pH and redox potential in the culture, these intermediates may exist as quinones or hydroquinones.
authors.affiliation: Department of Microbiology, Molecular Biology, and Biochemistry, University of Idaho, Moscow 83844, USA.
authors.forename: R H
authors.initials: RH
authors.lastname: Kaake
authors.forename: D L
authors.initials: DL
authors.lastname: Crawford
authors.forename: R L
authors.initials: RL
authors.lastname: Crawford
mesh_terms: D019297:2,4-Dinitrophenol / Q000031:analogs & derivatives*; D000107:Acetylation; D001420:Bacteria, Aerobic / Q000378:metabolism; D001673:Biodegradation, Environmental; D002457:Cell Extracts / Q000737:chemistry; D002845:Chromatography; D003470:Culture Media / Q000737:chemistry; D004140:Dinitrophenols / Q000378:metabolism*; D005990:Glycerol / Q000737:chemistry; D006540:Herbicides / Q000378:metabolism*; D008970:Molecular Weight; D011809:Quinones / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D012988:Soil Microbiology
chemical_list: D002457:Cell Extracts; D003470:Culture Media; D004140:Dinitrophenols; D006540:Herbicides; D011809:Quinones; D005990:Glycerol; D019297:2,4-Dinitrophenol; C018527:dinoseb