Mutations involving the endogenous ecotropic murine leukemia virus in primary mammary carcinomas of BALB/c mice.
Endogenous murine leukemia virus-related elements (MLVEs) are often overexpressed in primary mammary carcinomas of BALB/c mice. We therefore searched for mutations associated with MLVEs and found amplified sequences of the ecotropic MLVE in hormonally and chemically induced mammary neoplasms. Restriction fragment length polymorphism (RFLP) analysis revealed DNA rearrangements consistent with 1-10 or more new copies of the ecotropic MLVE in the genome of these tumors. This is the first evidence of mutations involving an endogenous retrovirus other than mouse mammary tumor virus in mouse mammary carcinomas.
Natoli
F
F
Department of Experimental Pathology, Roswell Park Cancer Institute, Buffalo, NY 14263-0001, USA.
Crowley
M R
MR
Asch
H L
HL
Stoler
D L
DL
Asch
B B
BB
D015127:9,10-Dimethyl-1,2-benzanthracene; D000818:Animals; D002273:Carcinogens; D003043:Cocarcinogenesis; D004273:DNA, Neoplasm / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D005260:Female; D005784:Gene Amplification; D009052:Leukemia Virus, Murine / Q000235:genetics*; D008321:Mammary Glands, Animal / Q000187:drug effects / Q000821:virology; D008325:Mammary Neoplasms, Experimental / Q000139:chemically induced / Q000821:virology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D009154:Mutation; D009376:Neoplasms, Hormone-Dependent / Q000821:virology; D012150:Polymorphism, Restriction Fragment Length; D011247:Pregnancy
D002273:Carcinogens; D004273:DNA, Neoplasm; D004279:DNA, Viral; D015127:9,10-Dimethyl-1,2-benzanthracene