title: CD30 expression does not discriminate between human Th1- and Th2-type T cells.
abstract: CD30 is a member of the TNF receptor superfamily that is commonly used as a marker for Hodgkin and Reed-Sternberg cells in Hodgkin's disease. More recently, it has been proposed that CD30 is preferentially up-regulated on Th2-type human T cells. We analyzed regulation of CD30 expression on both peripheral blood T cells and T cell clones. In short-term culture, CD30 expression could be induced on T cells by Ags that elicit Th2-type responses (Schistosoma haematobium, adult worm Ag, and Toxocaria canis, excretory/secretory Ag) and Th0-type responses (tetanus toxoid), as well as Th1-type responses (tuberculin purified protein derivative). Moreover, simultaneous measurement of membrane phenotype and cytokine production showed that CD30-expressing cells can produce IFN-gamma. Finally, within panels of randomly generated as well as Ag-specific T cell clones, CD30 expression was found on Th0-, Th2-, and Th1-type clones. We conclude that induction of CD30 on activated T cells is not related to differentiation in Th0-, Th1-, or Th2-type cells.
authors.affiliation: Central Laboratory of the Netherlands Red Cross Blood Transfusion Service, University of Amsterdam, The Netherlands.
authors.forename: D
authors.initials: D
authors.lastname: Hamann
authors.forename: C M
authors.initials: CM
authors.lastname: Hilkens
authors.forename: J L
authors.initials: JL
authors.lastname: Grogan
authors.forename: S M
authors.initials: SM
authors.lastname: Lens
authors.forename: M L
authors.initials: ML
authors.lastname: Kapsenberg
authors.forename: M
authors.initials: M
authors.lastname: Yazdanbakhsh
authors.forename: R A
authors.initials: RA
authors.lastname: van Lier
mesh_terms: D002454:Cell Differentiation; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis; D017730:Ki-1 Antigen / Q000378:metabolism*; D008213:Lymphocyte Activation; D018417:Th1 Cells / Q000276:immunology*; D018418:Th2 Cells / Q000276:immunology*; D015854:Up-Regulation
chemical_list: D017730:Ki-1 Antigen; D007371:Interferon-gamma