Microplate technique for screening and assessing cytotoxicity of xenobiotics with Tetrahymena pyriformis.
The microplate technique (MT) is developed and compared to the usual flask technique (FT) of Tetrahymena pyriformis GL cultures for assessing toxic effects of organic and inorganic substances on this ciliated protozoa model. Applied to the IC50 determination of 29 xenobiotics, the MT demonstrated good correlation with the FT (Y = 0.991X + 0.012, r = 0.94, P < 0.0001). The MT is rapid, easy-to-handle, inexpensive, and statistically reliable and, so, may be helpful for screening investigations of water-soluble xenobiotics.
Sauvant
M P
MP
Faculté de Pharmacie, Laboratoire Hydrologie & Hygiène, Clermont-Ferrand, France.
Pepin
D
D
Bohatier
J
J
Groliere
C A
CA
D000818:Animals; D001681:Biological Assay / Q000191:economics / Q000379:methods* / Q000592:standards; D003362:Cost-Benefit Analysis; D003470:Culture Media; D007928:Lethal Dose 50; D015203:Reproducibility of Results; D013053:Spectrophotometry; D013329:Structure-Activity Relationship; D013769:Tetrahymena pyriformis / Q000187:drug effects*; D015262:Xenobiotics / Q000633:toxicity*
D003470:Culture Media; D015262:Xenobiotics