title: Identification of alcA, a Bordetella bronchiseptica gene necessary for alcaligin production.
abstract: The alcA gene, essential for the production of the dihydroxamate siderophore, alcaligin, by Bordetella bronchiseptica, was cloned and sequenced. The alcA gene was identified on a 4.7-kb EcoRI genomic fragment adjacent to a Tn5lac transposon insertion that inactivated alcaligin production in strain MBORD846. Analysis of the alcA nucleotide sequence revealed a putative Fur-binding site, suggesting that expression of this gene is repressed by iron. The deduced amino-acid sequence of this open reading frame had significant homology with the Escherichia coli iucD gene product, an enzyme required for biosynthesis of the dihydroxamate siderophore aerobactin.
authors.affiliation: Department of Microbiology and Immunology, State University of New York at Buffalo 14214, USA.
authors.forename: P C
authors.initials: PC
authors.lastname: Giardina
authors.forename: L A
authors.initials: LA
authors.lastname: Foster
authors.forename: S I
authors.initials: SI
authors.lastname: Toth
authors.forename: B A
authors.initials: BA
authors.lastname: Roe
authors.forename: D W
authors.initials: DW
authors.lastname: Dyer
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D016950:Bordetella bronchiseptica / Q000235:genetics*; D003001:Cloning, Molecular; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D006877:Hydroxamic Acids; D007501:Iron / Q000502:physiology; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D017262:Siderophores / Q000096:biosynthesis*
chemical_list: D006877:Hydroxamic Acids; D012333:RNA, Messenger; D017262:Siderophores; C092056:alcaligin; D007501:Iron