Purification of a glucose/mannose specific lectin, isoform 1, from seeds of Cratylia mollis Mart. (Camaratu bean).
A quantitatively main molecular form of Cratylia mollis lectin, isoform 1 (iso 1) was purified by affinity chromatography on Sephadex G-75, followed by ion exchange chromatography on CM-cellulose. Another lectin form was identified in the latter step. Iso 1 is specific for glucose/mannose, with a main subunit of 31 kDa mol wt; the native protein is basic (pI 8.5-8.6) and the constituent polypeptides had a pI range of 5.15-7.75. An antibody to the protein was raised in a rabbit, and the conjugate was active in an immunosorbent assay.
Correia
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MT
Departamento de Bioquímica, Universidade Federal de Pernambuco, UFPE, PE, Brazil.
Coelho
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D000818:Animals; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000378:metabolism; D007887:Fabaceae / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D006384:Hemagglutination / Q000187:drug effects; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D005779:Immunodiffusion; D007074:Immunoglobulin G / Q000378:metabolism; D007525:Isoelectric Focusing; D037102:Lectins / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism; D008358:Mannose / Q000378:metabolism; D008970:Molecular Weight; D037121:Plant Lectins; D010946:Plants, Medicinal; D012639:Seeds / Q000378:metabolism*; D013237:Stereoisomerism
D007106:Immune Sera; D007074:Immunoglobulin G; D037102:Lectins; D037121:Plant Lectins; D005947:Glucose; D008358:Mannose