Presentation of antigen by B cell subsets. IV. Defective T-B cell signalling causes inability to present antigen by B cells from immunodeficient mice.
The purpose of this study was to investigate differences in T-B cell signalling between B cells from normal and immunodeficient mice. B cell blasts from normal and immunodeficient mice expressed comparable levels of membrane-associated IL-1. B cells from normal, but not immunodeficient mice, prefixed with glutaraldehyde and cultured with thymocytes or a T cell line BK33, induce in T cells production of a factor which causes release of IL-1 by macrophages. This factor, preincubated with B cells from immunodeficient mice significantly enhances their APC function. Furthermore, this cytokine induces expression of Lyb-5 alloantigen on B cells from immunodeficient mice. This effect could be blocked by neutralizing antibodies to IL-6 but not to IL-2, IL-4 or GM-CSF. We conclude that immature B cells from immunodeficient (CBA/N x BALB/c)F1 mice are unable to stimulate interacting T cells to produce IL-6 and therefore are inefficient antigen presenting cells.
Zimecki
M
M
Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław.
Kapp
J A
JA
D000818:Animals; D017951:Antigen Presentation / Q000502:physiology; D016175:B-Lymphocyte Subsets / Q000166:cytology* / Q000276:immunology*; D002450:Cell Communication / Q000502:physiology; D002460:Cell Line; D000939:Epitopes; D007153:Immunologic Deficiency Syndromes / Q000276:immunology / Q000473:pathology*; D007375:Interleukin-1 / Q000096:biosynthesis / Q000502:physiology; D015850:Interleukin-6 / Q000502:physiology; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008808:Mice, Inbred CBA; D015398:Signal Transduction / Q000502:physiology; D013601:T-Lymphocytes / Q000166:cytology* / Q000276:immunology
D000939:Epitopes; D007375:Interleukin-1; D015850:Interleukin-6