Immunohistochemical identification of proteoglycan form of macrophage colony-stimulating factor on bone surface.
Several studies using op/op mice have shown that macrophage colony-stimulating factor (M-CSF) was necessary for osteoclast formation in vivo. Previously we reported that osteoblastic cells produced two molecular forms of M-CSF; one is an 85-kDa M-CSF, and the other is a proteoglycan form of M-CSF (PG-M-CSF) which has a binding affinity for bone-derived collagens and is extractable from human bone. In this study, we performed immunostaining of human bone using a newly established anti-PG-M-CSF antibody, and showed positive staining PG-M-CSF, probably produced by bone lining cells, on the bone surface. This observation suggests that the bone surface is suitable for osteoclast formation because of the presence of PG-M-CSF.
Ohtsuki
T
T
Department of Internal Medicine, Jichi Medical School, Tochigi, Japan.
Hatake
K
K
Suzu
S
S
Saito
K
K
Motoyoshi
K
K
Miura
Y
Y
D000368:Aged; D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D001842:Bone and Bones / Q000378:metabolism*; D016466:CHO Cells; D006224:Cricetinae; D006801:Humans; D007150:Immunohistochemistry; D016173:Macrophage Colony-Stimulating Factor / Q000276:immunology / Q000378:metabolism*; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D011509:Proteoglycans / Q000378:metabolism*; D011817:Rabbits
D000911:Antibodies, Monoclonal; D011509:Proteoglycans; D016173:Macrophage Colony-Stimulating Factor