title: Effect of low-dose endotoxin on pulmonary vascular permeability following acute hemorrhagic shock.
abstract: The purpose of the current study was to determine the effect of low-dose Escherichia coli lipopolysaccharide (LPS) on pulmonary vascular permeability when administered after hemorrhagic shock (40% of baseline cardiac output) followed by resuscitation. Animals were monitored for 3-4 h after LPS infusion. Thirty minutes prior to termination of the experiment, 3 mCi of 125I-human serum albumin was injected intravenously to calculate a permeability index from the left lung lavage and plasma 125I ratios. The two control groups were (1) shock only (no LPS, n = 4), and (2) LPS only (no shock, n = 8). The permeability index for the shock-only group was 0.0015 +/- 0.0007 (mean +/- SE) and that for the LPS-only group was 0.0035 +/- 0.0014. The permeability index for the experimental group (shock followed by LPS, n = 10) was 0.0071 +/- 0.0030 (p > 0.05). Similarly, there was no difference in the wet-to-dry ratios between the three groups. The shock+LPS group required more intravenous fluids to maintain mean arterial blood pressure at control values than the LPS-only group (p < 0.003). We conclude that hemorrhagic shock and resuscitation do not lead to an acute increased permeability of the lung when it is subsequently challenged by a low dose of bacterial LPS.
authors.affiliation: Division of Pulmonary and Critical Care Medicine, University of Washington School of Medicine, Seattle 98195, USA.
authors.forename: Z L
authors.initials: ZL
authors.lastname: Xiao
authors.forename: D
authors.initials: D
authors.lastname: An
authors.forename: S
authors.initials: S
authors.lastname: Bernard
authors.forename: S
authors.initials: S
authors.lastname: Lakshminarayan
mesh_terms: D000818:Animals; D001427:Bacterial Toxins / Q000008:administration & dosage / Q000009:adverse effects*; D001794:Blood Pressure; D001831:Body Temperature; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D002199:Capillary Permeability / Q000187:drug effects*; D002302:Cardiac Output; D004926:Escherichia coli; D005440:Fluid Therapy; D006801:Humans; D007457:Iodine Radioisotopes / Q000032:analysis; D007958:Leukocyte Count; D008070:Lipopolysaccharides / Q000008:administration & dosage / Q000009:adverse effects*; D008168:Lung / Q000098:blood supply*; D009929:Organ Size; D010100:Oxygen / Q000097:blood; D011817:Rabbits; D012151:Resuscitation; D012709:Serum Albumin; D012771:Shock, Hemorrhagic / Q000503:physiopathology*
chemical_list: D001427:Bacterial Toxins; D007457:Iodine Radioisotopes; D008070:Lipopolysaccharides; D012709:Serum Albumin; D010100:Oxygen