title: The 2.0 A crystal structure of a heterotrimeric G protein.
abstract: The structure of a heterotrimeric G protein reveals the mechanism of the nucleotide-dependent engagement of the alpha and beta gamma subunits that regulates their interaction with receptor and effector molecules. The interaction involves two distinct interfaces and dramatically alters the conformation of the alpha but not of the beta gamma subunits. The location of the known sites for post-translational modification and receptor coupling suggest a plausible orientation with respect to the membrane surface and an activated heptahelical receptor.
authors.affiliation: Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06510, USA.
authors.forename: D G
authors.initials: DG
authors.lastname: Lambright
authors.forename: J
authors.initials: J
authors.lastname: Sondek
authors.forename: A
authors.initials: A
authors.lastname: Bohm
authors.forename: N P
authors.initials: NP
authors.lastname: Skiba
authors.forename: H E
authors.initials: HE
authors.lastname: Hamm
authors.forename: P B
authors.initials: PB
authors.lastname: Sigler
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D018360:Crystallography, X-Ray; D004926:Escherichia coli; D019204:GTP-Binding Proteins / Q000737:chemistry* / Q000378:metabolism; D006160:Guanosine Triphosphate / Q000378:metabolism; D006801:Humans; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011487:Protein Conformation; D011499:Protein Processing, Post-Translational; D011956:Receptors, Cell Surface / Q000737:chemistry / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry; D017386:Sequence Homology, Amino Acid
chemical_list: D011956:Receptors, Cell Surface; D011994:Recombinant Proteins; D006160:Guanosine Triphosphate; D019204:GTP-Binding Proteins