title: Human endometrial proteins with cyclic changes in the expression during the normal menstrual cycle: characterization by protein sequence analysis.
abstract: Endometrial proteins showing cyclic expression during the normal menstrual cycle were localized on two-dimensional (2-D) electrophoresis gels separating proteins with isoelectric points (pl) ranging from 3.5 to 7 and relative molecular weights ranging from 10 to 300 kDa. Menstrual cycle-related proteins were excised from several 2-D gels, concentrated by one-dimensional (1-D) sodium dodecyl sulphate (SDS)-polyacrylamide gel electrophoresis, and cleaved in situ by trypsin. The tryptic fragments were extracted and separated by reverse phase high performance liquid chromatography (RP-HPLC). Finally, the partial amino-terminal amino acid sequence of selected tryptic fragments were determined for each protein. We aimed at characterizing the 21 menstrual cycle-related proteins that were visible on silver-stained 2-D electrophoresis gels. Of the proteins being maximally synthesized in the proliferative phase endometrium, we identified proteins associated mainly with the cytoskeleton: vimentins, keratin, tropomyosin and tubulin, but also proteins such as proliferating cell nuclear antigen and beta-galactoside binding lectin. The partial amino acid sequences for another two proteins did not match any protein sequence in the Protein Identification Resource (PIR) and Swissprot databases. In the group of proteins having maximal synthesis in the secretory phase endometrium, we identified creatine kinase chain B and an isocitrate dehydrogenase-homologous protein, both of which are involved in energy metabolism. However, we also identified the annexin IV precursor, the 14-3-3 protein homologue also called stratifin or the epithelial cell marker protein 1 and the 21K tumour protein. Finally, four of the proteins were present in too low amounts to allow characterization. Interestingly, most of the identified proteins have not previously been described as having a menstrual cycle-related synthesis in the human endometrium. It may be considered that the concentration of some of the cycle-related proteins may be used in clinical situations to reflect specific endometrial phases.
authors.affiliation: Center for Clinical and Basic Research, Ballerup, Denmark.
authors.forename: I
authors.initials: I
authors.lastname: Byrjalsen
authors.forename: P M
authors.initials: PM
authors.lastname: Larsen
authors.forename: S J
authors.initials: SJ
authors.lastname: Fey
authors.forename: C
authors.initials: C
authors.lastname: Christiansen
mesh_terms: D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D037161:Galectins; D006388:Hemagglutinins / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D007526:Isoelectric Point; D007633:Keratins / Q000737:chemistry / Q000378:metabolism; D008597:Menstrual Cycle / Q000502:physiology*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D018809:Proliferating Cell Nuclear Antigen / Q000737:chemistry / Q000378:metabolism; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism*; D017421:Sequence Analysis; D014335:Tropomyosin / Q000737:chemistry / Q000378:metabolism; D014357:Trypsin / Q000378:metabolism; D014404:Tubulin / Q000737:chemistry / Q000378:metabolism; D014746:Vimentin / Q000737:chemistry / Q000378:metabolism
chemical_list: D037161:Galectins; D006388:Hemagglutinins; D010446:Peptide Fragments; D018809:Proliferating Cell Nuclear Antigen; D011506:Proteins; D014335:Tropomyosin; D014404:Tubulin; D014746:Vimentin; D007633:Keratins; D014357:Trypsin