Optimum culture conditions for production of exfoliative toxin by Staphylococcus hyicus.
Optimum culture conditions for the production of exfoliative toxin by Staphylococcus hyicus (shET) were examined. High shET activity was obtained from the culture filtrate of HI and TY broth inoculated with S. hyicus. The pH in these two media ranged from 7 to 8.5 during bacterial culture, while the lowest pH in TS and BHI broth was less than 6. shET activity in the culture filtrate from TY broth inoculated with 10(7) CFU of S. hyicus per ml was higher than that in TY broth inoculated with 10(6) and 10(8) CFU of bacteria per ml. When shET activity in the culture filtrate was measured under various shaking conditions, the culture filtrate shaken at 75 oscillations per min had the highest shET activity of the five shaking conditions. shET activity of the culture filtrate of TY broth to which protease inhibitor had been added was the same as that of TY broth without inhibitor. shET activity in a shaking culture in an Erlenmeyer flask was also the same as that in sac culture and that in shaking culture using a shaking (Sakaguchi) flask. shET activity in TY broth supplemented with 100 mM glucose was significantly lower than that in TY broth without glucose. Based on the above results, the optimum culture conditions for the production of shET were as follows: inoculation of 3 x 10(9) CFU of S. hyicus strain P-1 into 300 ml of TY broth in a 2,000-ml Erlenmeyer flask, and incubation at 37 C with shaking at 75 oscillations per min.(ABSTRACT TRUNCATED AT 250 WORDS)
Watanabe
T
T
Department of Veterinary Microbiology, School of Veterinary Medicine and Animal Sciences, Kitasato University, Aomori, Japan.
Tanabe
T
T
Sato
H
H
Fuse
Y
Y
Ueda
K
K
Nakano
K
K
Saito
H
H
Maehara
N
N
D000645:Ammonium Sulfate; D002478:Cells, Cultured; D015169:Colony Count, Microbial; D003470:Culture Media; D004848:Epithelium; D005083:Exfoliatins / Q000096:biosynthesis* / Q000302:isolation & purification; D005947:Glucose / Q000494:pharmacology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008828:Microbiological Techniques; D011480:Protease Inhibitors / Q000494:pharmacology; D013210:Staphylococcus / Q000187:drug effects / Q000502:physiology*
D003470:Culture Media; D005083:Exfoliatins; D011480:Protease Inhibitors; D005947:Glucose; D000645:Ammonium Sulfate