Effect of PQQ glucose dehydrogenase overexpression in Escherichia coli on sugar-dependent respiration.
Pyrroloquinoline quinone glucose dehydrogenase (PQQGDH) was overexpressed in Escherichia coli, and its impact on sugar-dependent respiration was investigated. Sugar-dependent respiration patterns under PQQGDH overexpression can be devided into two types. The first type involves D-glucose and D-mannose, which are utilized by the phosphotransferase system (PTS) and are also the substrates of PQQGDH. As a result of PQQGDH overexpression, the apparent Km value of sugar-dependent respiration shifted to higher concentration compared with E. coli parental cells. The second type included D-xylose and D-galactose, which are the substrates of PQQGDH, but not the PTS sugars. PQQGDH overexpressing cells showed much higher respiration than parental cells. These results suggested that PQQGDH overexpression may alter sugar utilization preferences in E. coli, suggesting further possible applications in metabolic engineering for carbon source utilization.
Sode
K
K
Department of Biotechnology, Faculty of Technology, Tokyo University of Agriculture and Technology, Japan.
Sugimoto
S
S
Watanabe
M
M
Tsugawa
W
W
D004566:Electrodes; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism*; D005690:Galactose / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D005948:Glucose Dehydrogenases / Q000096:biosynthesis* / Q000378:metabolism; D006601:Hexoses / Q000378:metabolism*; D008358:Mannose / Q000378:metabolism; D010101:Oxygen Consumption; D010770:Phosphotransferases / Q000378:metabolism; D015202:Protein Engineering / Q000379:methods; D013379:Substrate Specificity; D014994:Xylose / Q000378:metabolism*
D006601:Hexoses; D014994:Xylose; D005948:Glucose Dehydrogenases; C033955:glucose dehydrogenase (pyrroloquinoline-quinone); D010770:Phosphotransferases; D005947:Glucose; D008358:Mannose; D005690:Galactose