Mucin-type glycoprotein from Drosophila melanogaster embryonic cells: characterization of carbohydrate component.
A secreted glycoprotein (GP) with apparent molecular mass of 90 kDa produced by cultured embryonic cells of Drosophila melanogaster was isolated and partially characterized. GP is enriched by Ser + Thr and Pro residues that constitute up to 30% of the total number of amino acids. An abundant carbohydrate moiety (40% of molecular mass) is mainly represented by vertebrate mucin-type O-linked disaccharide units Gal(beta 1-3)-GalNAc, occupying about a half of the total number of Ser+Thr residues and rendering the GP molecule high resistance to protease action. A few of N-glycans are also present in GP. These characteristics allow to consider the Drosophila GP (termed 'mucin-D') as a first representative of invertebrate mucin-type glycoproteins.
Kramerov
A A
AA
Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, Russian Federation.
Arbatsky
N P
NP
Rozovsky
Y M
YM
Mikhaleva
E A
EA
Polesskaya
O O
OO
Gvozdev
V A
VA
Shibaev
V N
VN
D000581:Amidohydrolases / Q000378:metabolism / Q000494:pharmacology; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D015153:Blotting, Western; D001894:Borohydrides / Q000737:chemistry; D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000032:analysis; D002478:Cells, Cultured; D005591:Chemical Fractionation; D003208:Concanavalin A / Q000494:pharmacology; D004331:Drosophila melanogaster / Q000196:embryology*; D004625:Embryo, Nonmammalian / Q000737:chemistry* / Q000166:cytology*; D006031:Glycosylation; D006596:Hexosaminidases / Q000737:chemistry / Q000378:metabolism; D008969:Molecular Sequence Data; D009077:Mucins / Q000737:chemistry* / Q000378:metabolism; D009844:Oligosaccharides / Q000032:analysis / Q000737:chemistry; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase; D014415:Tunicamycin / Q000494:pharmacology
D000596:Amino Acids; D001894:Borohydrides; D002241:Carbohydrates; D009077:Mucins; D009844:Oligosaccharides; D003208:Concanavalin A; D014415:Tunicamycin; C025364:sodium borohydride; D006596:Hexosaminidases; C060638:glycopeptide alpha-N-acetylgalactosaminidase; D000581:Amidohydrolases; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase