Measurement of CD11/CD18 integrin expression on the polymorphonuclear cell surface after incubation with synthetic vascular prostheses.
Polymorphonuclear (PMN) cell activation in the presence of synthetic vascular grafts was studied by using flow cytometry to measure CD11/CD18 leukocyte adhesion molecule expression at the cell surface. Polymorphonuclear cells isolated from the blood of 10 healthy human donors were incubated with six knitted polyester vascular prostheses (Vasculour II, Albumin-Coated Graft, Triaxial, Gelatin-Impregnated Gelseal, Dialine I, and Bovine Collagen-Impregnated Dialine II) for 60 min at 37 degrees C. The authors' study demonstrated that CD11/CD18 integrin expression varies among donors for a given vascular prosthesis, and that each donor has his or her own pattern of responsiveness toward synthetic vascular grafts. The current study indicates that PMN activation measured by CD11/CD18 integrin expression is a sensitive method of measuring the blood compatibility of a given foreign surface for a given patient.
Jakubiec
B
B
Rheumatology Immunology Research Center, Laval University Medical Center, Québec, Canada.
Roy
R
R
Isles
M B
MB
Marois
Y
Y
Guidoin
R
R
D000818:Animals; D001672:Biocompatible Materials / Q000009:adverse effects; D001807:Blood Vessel Prosthesis / Q000009:adverse effects*; D018845:CD11 Antigens / Q000097:blood*; D018821:CD18 Antigens / Q000097:blood*; D002417:Cattle; D002462:Cell Membrane / Q000276:immunology; D006801:Humans; D066298:In Vitro Techniques; D008422:Materials Testing; D009504:Neutrophils / Q000276:immunology*; D011091:Polyesters / Q000009:adverse effects
D001672:Biocompatible Materials; D018845:CD11 Antigens; D018821:CD18 Antigens; D011091:Polyesters