Establishment of a rabbit short-term dry eye model.
The dry eye syndrome is a chronic disease which can become a serious threat to useful vision. However, there is only a limited understanding regarding effective treatment or prevention of this disease. Establishing an effective mode of treatment requires the use of a satisfactory animal dry eye model. Ideally, such a model should rapidly determine the effectiveness of agents that inhibit the damaging effects of this syndrome. This paper presents a short-term dry eye model using rabbits, which combines mechanical prevention of blinking and methylene blue staining. This model is not intended to be a precise representation of the dry eye syndrome, since this disorder has recently become recognized to involve a primary pathological process of the corneal and conjunctival epithelium. However, by using this model, clinical signs of dry eye can be observed after a few hours in the form of acute desiccation. Corneal damage can easily be evaluated both qualitatively by methylene blue staining scores, and quantitatively by chronic assay. Visually observed corneal epithelial thinning was confirmed by scanning electron microscopy (SEM) to be due to loss of epithelial integrity. Using a 3% chondroitin sulfate solution, an already proven effective agent for dry eye, this model effectively demonstrated an 80% inhibition in the development of methylene blue positive lesion after a period of only 2 hours. This short term dry eye model is valuable in primarily screening the efficacy of potential therapeutic agents in the prevention and treatment of dry eye.
Fujihara
T
T
Santen Pharmaceutical Co., Ltd., Ophthalmic Research Laboratories, Osaka, Japan.
Nagano
T
T
Nakamura
M
M
Shirasawa
E
E
D000818:Animals; D002809:Chondroitin Sulfates / Q000494:pharmacology; D004396:Coloring Agents; D003315:Cornea / Q000187:drug effects / Q000648:ultrastructure*; D003890:Desiccation; D004195:Disease Models, Animal; D015352:Dry Eye Syndromes / Q000188:drug therapy / Q000209:etiology / Q000473:pathology*; D004848:Epithelium / Q000187:drug effects / Q000648:ultrastructure; D008297:Male; D008751:Methylene Blue; D008855:Microscopy, Electron, Scanning; D009883:Ophthalmic Solutions; D011817:Rabbits
D004396:Coloring Agents; D009883:Ophthalmic Solutions; D002809:Chondroitin Sulfates; D008751:Methylene Blue