A chlorophyll synthetase gene from Arabidopsis thaliana.
During the course of an Arabidopsis thaliana genome sequencing project, we identified a gene, G4, with a derived amino acid sequence showing homology to the product of the Rhodobacter capsulatus bchG locus which is involved in the esterification of bacteriochlorophyllide with geranylgeraniol. The relationship between this gene and bchG was confirmed by the isolation and analysis of a corresponding full-length cDNA. Comparison of genomic and cDNA sequences indicated that the gene is made up of 14 exons, some of them being very short. Southern and Northern analyses showed that this sequence represents a single-copy gene and its transcript is detected only in green or greening tissues. Both homologies and expression data suggest that this gene encodes a chlorophyll synthetase, one of the last enzymes of chlorophyll biosynthesis, and thus represents a new example of a nuclear gene encoding an enzyme of this pathway in higher plants.
Gaubier
P
P
Laboratoire de Physiologie et Biologie Moléculaire des Plantes, URA 565 du CNRS, Université de Perpignan, France.
Wu
H J
HJ
Laudié
M
M
Delseny
M
M
Grellet
F
F
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics*; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D019729:Carbon-Oxygen Ligases; D003001:Cloning, Molecular; D018076:DNA, Complementary; D018744:DNA, Plant / Q000235:genetics / Q000302:isolation & purification; D005091:Exons; D017343:Genes, Plant; D008025:Ligases / Q000235:genetics*; D008969:Molecular Sequence Data; D018749:RNA, Plant / Q000032:analysis; D015183:Restriction Mapping; D016354:Rhodobacter capsulatus / Q000235:genetics; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D018744:DNA, Plant; D018749:RNA, Plant; D008025:Ligases; D019729:Carbon-Oxygen Ligases; C026901:chlorophyll synthetase