title: The Vitek immunodiagnostic assay for detection of immunoglobulin M toxoplasma antibodies.
abstract: We compared the Vitek immunodiagnostic assay for detection of immunoglobulin M (IgM) toxoplasma antibodies (VIDAS TXM) with an IgM enzyme-linked immunosorbent assay (ELISA) which we developed. A total of 407 serum samples from 391 adults were used for this study. We also examined 17 serial serum samples from four women who had seroconverted during gestation. We observed a sensitivity of 99.5%, a specificity of 93%, a positive predictive value of 93.6%, a negative predictive value of 99.5%, and a global agreement of 96.3%. In each case of seroconversion the VIDAS TXM became positive at the same time as did the IgM ELISA.
authors.affiliation: Department of Immunology and Infectious Diseases, Palo Alto Medical Foundation, CA 94301, USA.
authors.forename: E
authors.initials: E
authors.lastname: Candolfi
authors.forename: R
authors.initials: R
authors.lastname: Ramirez
authors.forename: M P
authors.initials: MP
authors.lastname: Hadju
authors.forename: C
authors.initials: C
authors.lastname: Shubert
authors.forename: J S
authors.initials: JS
authors.lastname: Remington
mesh_terms: D000328:Adult; D000818:Animals; D000913:Antibodies, Protozoan / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007075:Immunoglobulin M / Q000276:immunology*; D007159:Immunologic Tests; D008297:Male; D011247:Pregnancy; D011933:Reagent Kits, Diagnostic / Q000592:standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014122:Toxoplasma / Q000276:immunology*; D014123:Toxoplasmosis / Q000175:diagnosis* / Q000276:immunology*
chemical_list: D000913:Antibodies, Protozoan; D007075:Immunoglobulin M; D011933:Reagent Kits, Diagnostic