A screening system for antiviral compounds against herpes simplex virus type 1 using the MTT method with L929 cells.
We developed the improved MTT assay system for in vitro evaluation of anti-HSV-1 agents using L929 cells derived from mouse connective tissue. This assay system provides results in 4 days using a 96-well microplate. The EC50 values of several anti-HSV agents (ACV, BVaraU, and others) were found to be similar to those obtained by the plaque reduction method using MRC-5 cells. The present MTT assay is rapid, accurate, and may be useful as an automatic screening system for evaluation of anti-HSV-1 compounds.
Sudo
K
K
Rational Drug Design Laboratories, Fukushima, Japan.
Konno
K
K
Yokota
T
T
Shigeta
S
S
D000818:Animals; D000998:Antiviral Agents / Q000627:therapeutic use*; D002460:Cell Line; D004396:Coloring Agents; D003238:Connective Tissue / Q000187:drug effects; D003239:Connective Tissue Cells; D004353:Drug Evaluation, Preclinical; D005562:Formazans / Q000378:metabolism*; D006561:Herpes Simplex / Q000188:drug therapy*; D018259:Herpesvirus 1, Human; D007202:Indicators and Reagents; D051379:Mice; D015203:Reproducibility of Results; D013778:Tetrazolium Salts / Q000378:metabolism*; D013844:Thiazoles / Q000378:metabolism*; D013997:Time Factors
D000998:Antiviral Agents; D004396:Coloring Agents; D005562:Formazans; D007202:Indicators and Reagents; D013778:Tetrazolium Salts; D013844:Thiazoles; C022616:thiazolyl blue