Protochlorophyllide reductase. III: Synthesis of a protochlorophyllide-dihydroflavin complex.
A mild and efficient method of linking a dihydroflavin to the C-17 carboyxlic acid side chain of protochlorophyllide, without degradation of the sensitive E ring or loss of magnesium, is described. The appended dihydroflavin was shown to quench the fluorescence of protochlorophyllide. In contrast, a dihydronicotinamide moiety was unable to effect fluorescence quenching. the relevance of these findings to a possible mechanism of action of the enzyme protochlorophyllide reductase is discussed.
Nayar
P
P
Department of Chemistry, Cornell University, Ithaca, NY, USA.
Begley
T P
TP
D005415:Flavins / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D010088:Oxidoreductases / Q000737:chemistry*; D044925:Oxidoreductases Acting on CH-CH Group Donors; D010777:Photochemistry; D011521:Protochlorophyllide / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013816:Thermodynamics
D005415:Flavins; D011521:Protochlorophyllide; D010088:Oxidoreductases; D044925:Oxidoreductases Acting on CH-CH Group Donors; C023886:protochlorophyllide reductase