Lipid changes in HL-60 cells on differentiation into macrophages by treatment with a phorbol ester.
We studied changes in lipid composition of human promyelocytic leukemia cells (HL-60) on differentiation to the macrophage/monocytic lineage by treatment with the phorbol ester, 12-O-tetradecanoyl-phorbol-13-acetate. Differentiation was accompanied by: (i) a decrease in the level of phospholipids; (ii) a greater amount of triacylglycerols; (iii) an increase in 1-alk-1'-enyl-2-acyl- and 1-alkyl-2-acyl-sn-glycero-3-phosphoethanolamine and a decrease in 1-alkyl-2-acyl-sn-glycero-3-phosphocholine; and (iv) an increase in the level of arachidonic acid in ethanolamine phospholipids. The increased levels of ether-linked lipids and of arachidonic acid in ethanolamine phospholipids are consistent with an enhanced biosynthesis of platelet-activating factor and eicosanoids, which are particularly important in the macrophage function.
Manning
R
R
Department of Biological Sciences, University of Durham, England.
Fallani
A
A
Ruggieri
S
S
D016718:Arachidonic Acid / Q000378:metabolism; D002273:Carcinogens / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D002784:Cholesterol / Q000378:metabolism; D004983:Ethanolamines / Q000737:chemistry / Q000378:metabolism; D018922:HL-60 Cells / Q000737:chemistry / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D050356:Lipid Metabolism; D008264:Macrophages / Q000166:cytology*; D010743:Phospholipids / Q000378:metabolism; D010972:Platelet Activating Factor / Q000031:analogs & derivatives / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D014280:Triglycerides / Q000378:metabolism; D014407:Tumor Cells, Cultured
C036761:1-alkyl-2-acyl-sn-glycero-3-phosphocholine; D002273:Carcinogens; D004983:Ethanolamines; D010743:Phospholipids; D010972:Platelet Activating Factor; D014280:Triglycerides; D016718:Arachidonic Acid; C005448:phosphorylethanolamine; D002784:Cholesterol; D013755:Tetradecanoylphorbol Acetate