RACH2, a novel human gene that complements a fission yeast cell cycle checkpoint mutation.
We have identified a novel human gene by virtue of its ability to complement the rad1-1 checkpoint mutant of Schizosaccharomyces pombe. This gene, called RACH2, rescues the temperature-sensitive lethality of a rad1-1 wee1-50 double mutant of S. pombe. Expression of RACH2 in S. pombe rad1-1 strains partially restores UV resistance to the rad1-1 mutant strain. Expression of RACH2 in a rad1-1 cdc25-22 double mutant partially restores the dose-dependent delay in mitotic entry after irradiation that is lost in rad1-1 checkpoint-deficient mutants. Overexpression of RACH2 in human tissue culture cells induces apoptosis.
Davey
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Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, New York 11724, USA.
Beach
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D000595:Amino Acid Sequence; D017209:Apoptosis; D002453:Cell Cycle; D018797:Cell Cycle Proteins / Q000235:genetics; D002460:Cell Line; D003001:Cloning, Molecular; D016373:DNA, Antisense; D004268:DNA-Binding Proteins; D004720:Endonucleases / Q000235:genetics*; D005347:Fibroblasts; D005656:Fungal Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics* / Q000502:physiology*; D005796:Genes / Q000235:genetics*; D005816:Genetic Complementation Test; D006801:Humans; D015603:Keratinocytes; D026901:Membrane Transport Proteins; D008969:Molecular Sequence Data; D009154:Mutation; D010749:Phosphoprotein Phosphatases / Q000235:genetics; D012568:Schizosaccharomyces / Q000166:cytology* / Q000235:genetics / Q000528:radiation effects; D029702:Schizosaccharomyces pombe Proteins; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014466:Ultraviolet Rays; D020687:cdc25 Phosphatases; D000096982:Urea Transporters
D018797:Cell Cycle Proteins; D016373:DNA, Antisense; D004268:DNA-Binding Proteins; D004720:Endonucleases; D005656:Fungal Proteins; D026901:Membrane Transport Proteins; D010749:Phosphoprotein Phosphatases; D029702:Schizosaccharomyces pombe Proteins; D020687:cdc25 Phosphatases; D000096982:Urea Transporters; C499354:rad1 protein, S pombe