Anti-AIDS agents--XIX. Neotripterifordin, a novel anti-HIV principle from Tripterygium wilfordii: isolation and structural elucidation.
A new kaurane type diterpene lactone, neotripterifordin (1), has been isolated from the roots of Tripterygium wilfordii. The structure of 1 was elucidated by spectroscopic methods, which included the concerted application of a number of 2-D NMR techniques including 1H-1H COSY, phase-sensitive NOESY, HETCOR, and long-range HETCOR. Compound 1 showed potent anti-HIV replication activity in H9 lymphocyte cells with an EC50 of 25 nM and TI of 125.
Chen
K
K
Natural Products Laboratory, School of Pharmacy, University of North Carolina, Chapel Hill 27599, USA.
Shi
Q
Q
Fujioka
T
T
Nakano
T
T
Hu
C Q
CQ
Jin
J Q
JQ
Kilkuskie
R E
RE
Lee
K H
KH
D000818:Animals; D000998:Antiviral Agents / Q000302:isolation & purification* / Q000494:pharmacology*; D002417:Cattle; D002478:Cells, Cultured; D002681:China; D004224:Diterpenes / Q000302:isolation & purification* / Q000494:pharmacology; D015497:HIV-1 / Q000187:drug effects*; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects / Q000821:virology; D013058:Mass Spectrometry; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D018517:Plant Roots / Q000737:chemistry; D010947:Plants, Toxic / Q000737:chemistry*; D013055:Spectrophotometry, Infrared; D014779:Virus Replication / Q000187:drug effects
D000998:Antiviral Agents; D004224:Diterpenes; D010936:Plant Extracts; C097941:neotripterifordin