CD43 and CD5 antibodies define four normal and neoplastic B-cell subsets: a three-color flow cytometric study.
CD43 antibodies are best known for identifying normal and neoplastic T cells and a subset of neoplastic B cells in paraffin sections. The frequency of nonneoplastic CD43 + B cells in different reactive settings, the proportion of B-cell neoplasms with small CD43+ populations, and the relationship of CD43+ B cells was therefore studied in 138 specimens using three-color cytometric analysis. CD43 and CD5 defined four normal B-cell subsets (CD43 + CD5 +, CD43 + CD5-, CD43 - CD5+, and CD43-CD5-). A significantly greater proportion of CD43+ B cells was found in cord blood and putative HIV+ blood samples than in normal control bloods. B-cell neoplasms derived from each of these four B-cell subsets were identified, with CD43+ /CD5+ and CD43-/CD5- neoplasms being most common. The predominant B-cell population coexpressed CD43 alone in 2/39 neoplasms and CD5 alone in four. A minority of cases showed heterogeneous CD43 expression. The B cells in two of three posttransplant lymphoproliferative disorders coexpressed CD43. B cells showed weaker CD43 staining than did T-cells (relative fluorescence 0.38 +/- 0.29). These findings support the concept that CD43 expression by neoplastic B cells is not an aberrant finding. CD43 expression on normal and neoplastic B cells is independent of CD5 expression even though CD43 and CD5 are frequently coexpressed. CD43 expression by B cells sometimes might be underestimated in paraffin sections because it is much weaker than on T cells.(ABSTRACT TRUNCATED AT 250 WORDS)
Lynch
E F
EF
Department of Pathology, University of Pittsburgh School of Medicine, Pennsylvania, USA.
Jones
P A
PA
Swerdlow
S H
SH
D000328:Adult; D000643:Ammonium Chloride; D000818:Animals; D000906:Antibodies / Q000276:immunology*; D015703:Antigens, CD; D018941:Antigens, CD19 / Q000276:immunology; D016175:B-Lymphocyte Subsets / Q000276:immunology*; D018956:CD5 Antigens / Q000276:immunology*; D005434:Flow Cytometry; D006801:Humans; D051917:Leukosialin; D016393:Lymphoma, B-Cell / Q000276:immunology*; D011933:Reagent Kits, Diagnostic; D012795:Sialoglycoproteins / Q000276:immunology*
D000906:Antibodies; D015703:Antigens, CD; D018941:Antigens, CD19; D018956:CD5 Antigens; D051917:Leukosialin; D011933:Reagent Kits, Diagnostic; C465014:SPN protein, human; D012795:Sialoglycoproteins; D000643:Ammonium Chloride