title: Periaxin expression in myelinating Schwann cells: modulation by axon-glial interactions and polarized localization during development.
abstract: Periaxin is a newly described protein that is expressed exclusively by myelinating Schwann cells. In developing nerves, periaxin is first detected as Schwann cells ensheathe axons, prior to the appearance of the proteins that characterize the myelin sheath. Periaxin is initially concentrated in the adaxonal membrane (apposing the axon) but, during development, as myelin sheaths mature, periaxin becomes predominately localized at the abaxonal Schwann cell membrane (apposing the basal lamina). In permanently axotomized adult nerves, periaxin is lost from the abaxonal and adaxonal membranes, becomes associated with degenerating myelin sheaths and is phagocytosed by macrophages. In crushed nerves, in which axons regenerate and are remyelinated, periaxin is first detected in the adoxonal membrane as Schwann cells ensheathe regenerating axons, but again prior to the appearance of other myelin proteins. Periaxin mRNA and protein levels change in parallel with those of other myelin-related genes after permanent axotomy and crush. These data demonstrate that periaxin is expressed by myelinating Schwann cells in a dynamic, developmentally regulated manner. The shift in localization of periaxin in the Schwann cell after completion of the spiralization phase of myelination suggests that periaxin participates in membrane-protein interactions that are required to stabilize the mature myelin sheath.
authors.affiliation: Department of Neurology, University of Pennsylvania School of Medicine, Philadelphia 19104-6146, USA.
authors.forename: S S
authors.initials: SS
authors.lastname: Scherer
authors.forename: Y T
authors.initials: YT
authors.lastname: Xu
authors.forename: P G
authors.initials: PG
authors.lastname: Bannerman
authors.forename: D L
authors.initials: DL
authors.lastname: Sherman
authors.forename: P J
authors.initials: PJ
authors.lastname: Brophy
mesh_terms: D000818:Animals; D001369:Axons / Q000502:physiology*; D015152:Blotting, Northern; D007150:Immunohistochemistry; D008565:Membrane Proteins / Q000502:physiology*; D016253:Microscopy, Immunoelectron; D009186:Myelin Sheath; D009457:Neuroglia / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017475:Receptors, Nerve Growth Factor / Q000502:physiology; D012583:Schwann Cells / Q000502:physiology*; D014855:Wallerian Degeneration
chemical_list: D008565:Membrane Proteins; D017475:Receptors, Nerve Growth Factor; C086745:periaxin