Use of a renal tubule cell line (LLC-PK1) to study the nephrotoxic potential of a kappa-type Bence-Jones protein.
The cytotoxicity of a Bence-Jones protein was assessed using a porcine renal tubule cell line (LLC-PK1), with the aim of developing a model for studying the potential nephrotoxicity of these proteins. The effects of a kappa Bence-Jones protein on cell viability were studied by means of biochemical methods (supravital dye uptake and measurement of cellular enzyme activities) and morphological electron microscopy. After a 24-h-treatment with Bence-Jones protein, a moderate cytotoxicity (about 15%) was noted but only a minor difference compared to treatment with bovine albumin in the same conditions. The morphological study showed a few cells in the process of lysis, but their numbers were insufficient for the demonstration of a clear cytotoxic effect. Immunocytochemical studies showed Bence-Jones protein fixation on some cells, especially on the outer membrane. Labeling of the hyaloplasm and basal pole of a few cells pointed to internalization of protein by LLC-PK1 cells. Although the cytotoxicity of the Bence-Jones protein tested here was only moderate, the use of this model enabled its cytotoxic effect to be distinguished from that of beta-lactoglobulin. This isolate could serve as a "moderate control" for a later study with a BJP having caused acute renal failure.
Diemert
M C
MC
Laboratoire d'Immunochimie, Hôpital Pitié-Salpêtrière, Paris, France.
Tricottet
V
V
Benel
L
L
Descamps
G
G
Escolano
E
E
Galli
J
J
Reynès
M
M
Rousselet
F
F
D000818:Animals; D001536:Bence Jones Protein / Q000633:toxicity*; D002417:Cattle; D002470:Cell Survival / Q000187:drug effects; D006801:Humans; D007687:Kidney Tubules, Proximal / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology / Q000648:ultrastructure; D018374:LLC-PK1 Cells; D007782:Lactoglobulins / Q000494:pharmacology; D008297:Male; D008875:Middle Aged; D012710:Serum Albumin, Bovine / Q000494:pharmacology; D013552:Swine
D007782:Lactoglobulins; D012710:Serum Albumin, Bovine; D001536:Bence Jones Protein