The effect of coculture on the postfertilization development of in vitro-matured monkey oocytes.
OBJECTIVE
To determine if the developmental potential of embryos resulting from in vivo- and in vitro-matured monkey oocytes could be increased through the use of a coculture system.


METHODS
Randomized prospective comparison of embryos resulting from either in vitro- or in vivo-matured oocytes cocultured with Vero cells or cultured in medium alone (control).


METHODS
Basic research laboratory.


METHODS
In vitro embryo development to the blastocyst stage and blastocyst hatching.


RESULTS
No significant difference in development was noted between coculture and control groups with embryos resulting from in vivo-matured oocytes. However, coculture was found to improve significantly the development of monkey embryos resulting from in vitro-matured oocytes.


CONCLUSIONS
These results demonstrate that primate embryos resulting from in vitro-matured and in vitro-fertilized oocytes differ in their culture requirement when compared with embryos resulting from in vivo-matured oocytes.
Lanzendorf
S E
SE
Jones Institute for Reproductive Medicine, Eastern Virginia Medical School, Norfolk 23507, USA.
Gordon
K
K
Mahony
M
M
Boyd
C A
CA
Neely
B
B
Hodgen
G D
GD
D000818:Animals; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D005260:Female; D008252:Macaca fascicularis; D009865:Oocytes; D011897:Random Allocation; D014709:Vero Cells; D015053:Zygote / Q000254:growth & development*