Rapid genotyping of low density lipoprotein receptor knockout mice using a polymerase chain reaction technique.
A new method for the rapid genotyping of low density lipoprotein receptor knockout mice is described. This method using a polymerase chain reaction (PCR) technique may be performed on small tissue biopsies, and represents a significant advantage in time over conventional genotyping by Southern blot analyses.
Gaw
A
A
Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas 75235, USA.
Mancini
F P
FP
Ishibashi
S
S
D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D004247:DNA / Q000032:analysis; D004195:Disease Models, Animal; D005838:Genotype; D006938:Hyperlipoproteinemia Type II; D051379:Mice; D018345:Mice, Knockout / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D011973:Receptors, LDL / Q000235:genetics*
D011973:Receptors, LDL; D004247:DNA