title: White matter microglia produce membrane-type matrix metalloprotease, an activator of gelatinase A, in human brain tissues.
abstract: Membrane-type matrix metalloprotease (MT-MMP) is an activator of gelatinase A (MMP-2), which has previously been found in carcinoma cells. We examined non-neurological and Alzheimer's disease brain tissues for MT-MMP by immunohistochemistry and in situ hybridization. The anti-MT-MMP antibodies gave positive staining of brain microglial cells in all the brain tissues. Positively stained microglia were found only in the white matter. The cells producing MT-MMP protein were also shown to be white matter microglia. These results provide further evidence that activated gelatinase A, which may be a processing enzyme for degradation of beta-amyloid protein, may be produced in white matter microglia.
authors.affiliation: Department of Neurology, Chiba University, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Yamada
authors.forename: Y
authors.initials: Y
authors.lastname: Yoshiyama
authors.forename: H
authors.initials: H
authors.lastname: Sato
authors.forename: M
authors.initials: M
authors.lastname: Seiki
authors.forename: A
authors.initials: A
authors.lastname: Shinagawa
authors.forename: M
authors.initials: M
authors.lastname: Takahashi
mesh_terms: D000368:Aged; D000544:Alzheimer Disease / Q000201:enzymology*; D001483:Base Sequence; D015153:Blotting, Western; D001921:Brain / Q000201:enzymology*; D005260:Female; D018093:Gelatinases / Q000378:metabolism*; D006801:Humans; D017403:In Situ Hybridization; D008297:Male; D020778:Matrix Metalloproteinase 2; D008666:Metalloendopeptidases / Q000378:metabolism*; D017628:Microglia / Q000201:enzymology*; D008875:Middle Aged; D008969:Molecular Sequence Data
chemical_list: D018093:Gelatinases; D008666:Metalloendopeptidases; D020778:Matrix Metalloproteinase 2