Structure of the O-specific side chain of the Escherichia coli O128 lipopolysaccharide.
The O-specific polysaccharide isolated from Escherichia coli O128 lipopolysaccharide contains D-galactose, L-fucose, and 2-acetamido-2-deoxy-D-galactose in the molar ratios 2:1:2. The primary structure of the O-specific polysaccharide from E. coli was established by compositional analysis, methylation analysis, together with 1H and 13C NMR spectroscopy including two-dimensional shift-correlated and one-dimensional NOE spectroscopy. The polysaccharide moiety was found to consist of a tetrasaccharide backbone containing D-galactose and 2-acetamido-2-deoxy-D-galactose, with L-fucose as a side chain in a branched pentasaccharide repeating unit having the following structure: [formula: see text]
Sengupta
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Department of Biological Chemistry, Indian Association for the Cultivation of Science, Jadavpur, Calcutta, India.
Bhattacharyya
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Shashkov
A S
AS
Kochanowski
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Basu
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D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D004926:Escherichia coli / Q000276:immunology*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009005:Monosaccharides / Q000032:analysis; D019081:O Antigens / Q000737:chemistry* / Q000302:isolation & purification; D009844:Oligosaccharides / Q000737:chemistry
D009005:Monosaccharides; D019081:O Antigens; D009844:Oligosaccharides