title: Elevated IL-8 production by trauma patients' monocytes is associated with elevated secretion of TNF alpha.
abstract: IL-8 is a recently described chemokine that increases polymorphonuclear neutrophil infiltration and has been implicated in inflammatory pathology. This study assesses monocyte (M phi) interleukin-8 (IL-8) levels in severe trauma patients (injury severity score > 16) who have elevated levels of M phi cell-associated tumor necrosis factor alpha (TNF alpha), a major marker for systemic inflammatory response syndrome after injury. We demonstrate elevated (p = .0007) levels of M phi IL-8 only in those trauma patients who also have increased (p = .0001) M phi-secreted TNF alpha whereas the patients having normal M phi-secreted TNF alpha levels have normal or even decreased M phi IL-8 production. There is no association between M phi IL-8 production and cell-associated TNF alpha levels. M phi induction by Fc gamma RI cross-linking, a common induction pathway in trauma patients' M phi that increases the production of both cell-associated and secreted TNF alpha, can also increase (p = .0022) M phi IL-8 levels. Therefore, post-trauma elevation of M phi IL-8 levels may be associated with increased secreted TNF alpha resulting from, at least in part, Fc gamma RI cross-linking stimulation in vivo.
authors.affiliation: Department of Surgery, University of Massachusetts Medical Center, Worcester 01655, USA.
authors.forename: A K
authors.initials: AK
authors.lastname: De
authors.forename: K
authors.initials: K
authors.lastname: Kodys
authors.forename: J C
authors.initials: JC
authors.lastname: Puyana
authors.forename: G
authors.initials: G
authors.lastname: Fudem
authors.forename: P
authors.initials: P
authors.lastname: Savoie
authors.forename: C L
authors.initials: CL
authors.lastname: Miller-Graziano
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D016209:Interleukin-8 / Q000096:biosynthesis*; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000378:metabolism*; D017452:Receptors, IgG / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*; D014947:Wounds and Injuries / Q000378:metabolism* / Q000473:pathology
chemical_list: D016209:Interleukin-8; D017452:Receptors, IgG; D014409:Tumor Necrosis Factor-alpha