Relationship between infectious cell-associated human immunodeficiency virus type 1 load, T lymphocyte subsets, and stage of infection in homosexual men.
The relationship between cell-associated infectious human immunodeficiency virus type 1 (HIV) load (infectious units/10(6) peripheral blood mononuclear cells [IUPM]) and phenotypes of CD4+ and CD8+ lymphocytes was studied. IUPM were measured in 242 HIV-infected homosexual men by quantitative microculture and T cell subsets by two-color flow cytometry. In multivariate analysis, IUPM correlated negatively with CD4+ lymphocyte level and with a diagnosis of AIDS and positively with the proportion of CD8+ lymphocytes expressing the activation marker CD38. After adjusting for level of CD4+ lymphocytes, men with AIDS had significantly lower IUPM than those without AIDS. The correlation between IUPM and CD4+ lymphocyte level was largely explained by correlation with level of CD4+ lymphocytes with resting phenotypes (HLA-DR-, CD38-) rather than with those expressing HLA-DR and CD38. Thus, subsets of CD4+ lymphocytes may vary in cell-associated infectious HIV content at different stages of HIV infection.
Margolick
J B
JB
Department of Molecular Microbiology and Immunology, Johns Hopkins University School of Hygiene and Public Health, Baltimore 21205, USA.
Farzadegan
H
H
Hoover
D R
DR
Saah
A J
AJ
D000163:Acquired Immunodeficiency Syndrome / Q000276:immunology* / Q000503:physiopathology / Q000821:virology; D015703:Antigens, CD / Q000276:immunology; D018791:CD4 Lymphocyte Count; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D003430:Cross-Sectional Studies; D005434:Flow Cytometry; D015497:HIV-1 / Q000502:physiology*; D006684:HLA-DR Antigens / Q000276:immunology; D018451:Homosexuality, Male; D006801:Humans; D016130:Immunophenotyping; D007963:Leukocytes, Mononuclear / Q000821:virology; D008297:Male; D016176:T-Lymphocyte Subsets / Q000276:immunology*
D015703:Antigens, CD; D006684:HLA-DR Antigens