Transforming growth factor-alpha in human submandibular gland and saliva.
A sensitive sandwich enzyme immunoassay (EIA) for transforming growth factor-alpha (TGF-alpha) utilizing a polyclonal antibody that recognizes limited epitopes of both human TGF-alpha and rat TGF-alpha in combination with a monoclonal anti-TGF-alpha IgG1 galactosidase conjugate was developed. This assay shows no cross-reactivity with human epidermal growth factor. We can quantify the TGF-alpha level in not only human TGF-alpha (detection limit: 1 pg/ml), but also rat TGF-alpha (detection limit: 10 pg/ml) by virtue of cross-reactivity. Employing this assay system, we demonstrated that TGF-alpha is present in both human submandibular glands and submandibular/sublingual saliva.
Mogi
M
M
Department of Oral Biochemistry, Matsumoto Dental College, Shiojiri, Japan.
Inagaki
H
H
Kojima
K
K
Minami
M
M
Harada
M
M
D000818:Animals; D006801:Humans; D007124:Immunoenzyme Techniques / Q000592:standards; D008297:Male; D008545:Melanoma / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D012463:Saliva / Q000737:chemistry / Q000276:immunology*; D013363:Submandibular Gland / Q000737:chemistry / Q000276:immunology*; D016211:Transforming Growth Factor alpha / Q000032:analysis*; D014407:Tumor Cells, Cultured
D016211:Transforming Growth Factor alpha