title: Evaluation of ractopamine cross-reactivity in several commercially available beta-agonist enzyme immunoassay kits.
abstract: Several enzyme immunoassay test kits are commercially available for screening of matrices including cattle feed, tissues, faeces and urine for the presence of beta-agonists. The kits utilized for this evaluation offer sensitivity as low as 0.1 ng ml-1 (assay solution concentration, not test sample). Evaluation of ractopamine hydrochloride (LY31537) solutions at concentrations as high as 1000 ng ml-1, using six different kits from four different manufacturers, showed cross-reactivity of less than 0.5%.
authors.affiliation: Lilly Research Laboratories, A Division of Eli Lilly and Company, Greenfield, IN 46140-0708, USA.
authors.forename: A L
authors.initials: AL
authors.lastname: Wicker
authors.forename: M P
authors.initials: MP
authors.lastname: Turberg
authors.forename: M R
authors.initials: MR
authors.lastname: Coleman
mesh_terms: D000318:Adrenergic beta-Agonists / Q000032:analysis* / Q000276:immunology; D003429:Cross Reactions; D005069:Evaluation Studies as Topic; D007124:Immunoenzyme Techniques; D010627:Phenethylamines / Q000032:analysis* / Q000276:immunology; D011933:Reagent Kits, Diagnostic
chemical_list: D000318:Adrenergic beta-Agonists; D010627:Phenethylamines; D011933:Reagent Kits, Diagnostic; C060897:ractopamine