A Schistosoma mansoni fatty acid-binding protein, Sm14, is the potential basis of a dual-purpose anti-helminth vaccine.
Molecular cloning of components of protective antigenic preparations has suggested that related parasite fatty acid-binding proteins could form the basis of the protective immune crossreactivity between the parasitic trematode worms Fasciola hepatica and Schistosoma mansoni. Molecular models of the two parasite proteins showed that both molecules adopt the same basic three-dimensional structure, consisting of a barrel-shaped molecule formed by 10 antiparallel beta-pleated strands joined by short loops, and revealed the likely presence of crossreactive, discontinuous epitopes principally derived from amino acids in the C-terminal portions of the molecules. A recombinant form of the S. mansoni antigen, rSm14, protected outbred Swiss mice by up to 67% against challenge with S. mansoni cercariae in the absence of adjuvant and without provoking any observable autoimmune response. The same antigen also provided complete protection against challenge with F. hepatica metacercariae in the same animal model. The results suggest that it may be possible to produce a single vaccine that would be effective against at least two parasites, F. hepatica and S. mansoni, of veterinary and human importance, respectively.
Tendler
M
M
Instituto Oswaldo Cruz-Fundação Oswaldo Cruz, Rio de Janeiro, Brazil.
Brito
C A
CA
Vilar
M M
MM
Serra-Freire
N
N
Diogo
C M
CM
Almeida
M S
MS
Delbem
A C
AC
Da Silva
J F
JF
Savino
W
W
Garratt
R C
RC
Katz
N
N
Simpson
A S
AS
D000595:Amino Acid Sequence; D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology*; D002352:Carrier Proteins; D003429:Cross Reactions; D018604:Epitope Mapping; D005210:Fasciola hepatica / Q000276:immunology; D050612:Fatty Acid Transport Proteins; D015801:Helminth Proteins / Q000276:immunology*; D006801:Humans; D026901:Membrane Transport Proteins; D051379:Mice; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011817:Rabbits; D012550:Schistosoma mansoni / Q000737:chemistry / Q000276:immunology*; D012555:Schistosomiasis mansoni / Q000517:prevention & control; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014614:Vaccines, Synthetic
D000947:Antigens, Helminth; D002352:Carrier Proteins; D050612:Fatty Acid Transport Proteins; D015801:Helminth Proteins; D026901:Membrane Transport Proteins; D014614:Vaccines, Synthetic; C068709:SM14 protein, Schistosoma mansoni