Biosynthesis of the so-called "a" and "asialo" pathway glycosphingolipids is differentially regulated in murine myelogenous leukemia NFS60 cells.
Regulation of "a" and "asialo" series ganglioside biosynthesis was analyzed. COS-1 cells expressing only GD1a showed high synthetic activities of GM3, GM2, GM1a, and GD1a, but little activity for GA2 synthesis. However, IL-3-dependent murine NFS60-I7, which has GM1b and GD1 alpha, exhibited high synthetic activities of GM2, GM1a, and GD1a, but GM3 synthase was only 1/6 of COS-1 and GA2 synthetic activity was low. By contrast, IL-3 gene-transfected subline NFS60-H7 expressing GD1a in addition to GM1b and GD1 alpha displayed up-regulated GM3 synthase and GA2 synthase activities, while GM2, GM1a, and GD1a synthase activities were in the same levels as in NFS60-I7 cells. Since GA2 synthetic activities were not parallel with GM2 synthase in the investigated machinery of ganglioside biosynthesis, it is strongly suggested that biosynthesis of "a" and "asialo" series gangliosides is regulated differentially from each other in murine NFS60 cell lines.
Nakamura
M
M
Division of Hemopoiesis, Institute of Hematology, Jichi Medical School, Tochigi, Japan.
Sakai
T
T
Furukawa
Y
Y
Kitagawa
S
S
Sakoe
K
K
Tsunoda
A
A
D000818:Animals; D002240:Carbohydrate Sequence; D002460:Cell Line; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D005678:G(M2) Ganglioside / Q000096:biosynthesis; D005732:Gangliosides; D006028:Glycosphingolipids / Q000096:biosynthesis* / Q000378:metabolism; D016695:Glycosyltransferases / Q000378:metabolism*; D008546:Melanoma, Experimental / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D013379:Substrate Specificity
D005732:Gangliosides; D006028:Glycosphingolipids; D005678:G(M2) Ganglioside; C038244:ganglio-N-triaosylceramide; D016695:Glycosyltransferases