Auditory brainstem responses of CBA/J mice with neonatal conductive hearing losses and treatment with GM1 ganglioside.
Exogenous administration of GM1 ganglioside to CBA/J mice with a neonatal conductive hearing loss ameliorates the atrophy of spiral ganglion neurons, ventral cochlear nucleus neurons, and ventral cochlear nucleus volume. The present investigation demonstrates the extent of a conductive loss caused by atresia and tests the hypothesis that GM1 ganglioside treatment will ameliorate the conductive hearing loss. Auditory brainstem responses were recorded from four groups of seven mice each: two groups received daily subcutaneous injections of saline (one group had normal hearing; the other had a conductive hearing loss); the other two groups received daily subcutaneous injections of GM1 ganglioside (one group had normal hearing; the other had a conductive hearing loss). In mice with a conductive loss, decreases in hearing sensitivity were greatest at high frequencies. The decreases were determined by comparing mean ABR thresholds of the conductive loss mice with those of normal hearing mice. The conductive hearing loss induced in the mice in this study was similar to that seen in humans with congenital aural atresias. GM1 ganglioside treatment had no significant effect on ABR wave I thresholds or latencies in either group.
Money
M K
MK
Louisiana State University Medical Center, Department of Otorhinolaryngology and Biocommunication Kresge Hearing Research Laboratory of the South, New Orleans 70112-2234, USA.
Pippin
G W
GW
Weaver
K E
KE
Kirsch
J P
JP
Webster
D B
DB
D000161:Acoustic Stimulation; D000818:Animals; D001309:Auditory Threshold / Q000187:drug effects*; D017626:Cochlear Nucleus / Q000166:cytology / Q000187:drug effects; D004195:Disease Models, Animal; D016057:Evoked Potentials, Auditory, Brain Stem / Q000187:drug effects* / Q000502:physiology; D005677:G(M1) Ganglioside / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D006314:Hearing Loss, Conductive / Q000188:drug therapy* / Q000503:physiopathology; D051379:Mice; D008808:Mice, Inbred CBA; D009474:Neurons / Q000166:cytology / Q000187:drug effects; D013136:Spiral Ganglion / Q000166:cytology / Q000187:drug effects; D014432:Tympanic Membrane / Q000187:drug effects
D005677:G(M1) Ganglioside