title: Analysis of Staphylococcus aureus genes encoding penicillin-binding protein 4 and an ABC-type transporter.
abstract: A region of the Staphylococcus aureus chromosome has been isolated that contains the gene encoding penicillin-binding protein 4 (PBP4), as well as abcA, a gene that encodes a protein with strong sequence similarity to the ABC transporter family of proteins. A disruption in abcA by Campbell-type integration results in cells that display an increased resistance to methicillin and cefoxitin, two antibiotics known to interact with low-molecular-weight PBPs. Based on these observations, a potential regulatory link between these two genes is discussed.
authors.affiliation: Department of Biological Sciences, University of Maryland, Baltimore 21228, USA.
authors.forename: T L
authors.initials: TL
authors.lastname: Domanski
authors.forename: K W
authors.initials: KW
authors.lastname: Bayles
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics*; D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D005786:Gene Expression Regulation; D005798:Genes, Bacterial; D006602:Hexosyltransferases; D008969:Molecular Sequence Data; D002267:Muramoylpentapeptide Carboxypeptidase / Q000235:genetics*; D016254:Mutagenesis, Insertional; D046915:Penicillin-Binding Proteins; D010458:Peptidyl Transferases; D012333:RNA, Messenger / Q000235:genetics; D013211:Staphylococcus aureus / Q000235:genetics*; D018440:beta-Lactam Resistance
chemical_list: D018528:ATP-Binding Cassette Transporters; D001426:Bacterial Proteins; D002352:Carrier Proteins; D046915:Penicillin-Binding Proteins; D012333:RNA, Messenger; D010458:Peptidyl Transferases; D006602:Hexosyltransferases; D002267:Muramoylpentapeptide Carboxypeptidase