Enhancement of SCE frequency by alpha-naphthoflavone in cultured lymphocytes in relation to environmental factors.
One hundred and nine healthy subjects living in an urban area of Tuscany were monitored using sister chromatid exchange (SCE) analysis on lymphocytes cultured in standard or alpha-naphthoflavone (ANF)-supplemented medium in order to collect the most complete data possible for those constitutional and environmental factors with which genotoxic risk can be associated. ANF genotoxicity depends on its metabolic activation by cellular P-450 monooxygenase systems whose activity can be modulated by exposure to carcinogenic but nongenotoxic xenobiotics. Lymphocytes grown in standard conditions showed a significant increase of SCE frequency associated with smoking habits and age. Although the addition of ANF caused an upward shift of SCE frequency in all subjects, smokers, coffee drinkers, and blue-collar workers showed a significantly higher SCE level; this suggests that potential risk factors rising from a modified cell metabolism are present in these categories. These results indicate that in vitro ANF treatment of lymphocytes could be a useful tool in the detection of environmental exposure to those classes of chemicals involved in metabolic activation of promutagens.
Sbrana
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I
Dipartimento di Scienze dell'Ambiente e del Territorio, Università di Pisa, Italy.
Luccini
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A
Landi
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S
Barale
R
R
D001571:Benzoflavones / Q000494:pharmacology*; D001711:Biotransformation; D002478:Cells, Cultured; D003069:Coffee; D004777:Environment; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D008297:Male; D009153:Mutagens / Q000494:pharmacology*; D012016:Reference Values; D012044:Regression Analysis; D012727:Sex Characteristics; D012854:Sister Chromatid Exchange / Q000187:drug effects*; D012907:Smoking; D012959:Socioeconomic Factors
D001571:Benzoflavones; D003069:Coffee; D009153:Mutagens; C011512:alpha-naphthoflavone