title: The repertoire of T-cell receptors in systemic sclerosis.
abstract: Certain T cell subsets are increased in systemic sclerosis patients, particularly V delta 1+ gamma delta T cells in the blood and lungs and CD8+ alpha beta T cells in the lungs. The repertoires of T cell antigen receptor (TCR) V delta 1, V alpha, and V beta gene families were examined by two methods of analysis. First, the relative abundance of V alpha and V beta gene transcripts was determined in the blood and bronchoalveolar fluid of the patients. Second, the diversity of the junctional regions in TCR V delta 1 transcripts and in different V alpha and V beta gene families was analyzed. Limited V delta 1-C delta junctional region lengths were observed in the patients compared with controls. This was confirmed by sequence analysis of V delta 1-C delta junctional regions after subcloning amplified products in a bacterial vector. Evidence for selection of the V delta 1+ T cells in the tissues of patients came from the findings that the same V delta 1-C delta junctional sequences persisted in an individual patient over time and that identical junctional sequences were isolated from multiple sites. A restricted diversity of the junctional region lengths was also detected in a number of V alpha and V beta gene families, particularly within bronchoalveolar CD8+ T cell subset. These data suggest that the oligoclonal expansion of the corresponding alpha beta and gamma delta T cells is antigen-driven and may be important in the pathogenesis of systemic sclerosis.
authors.affiliation: University of Maryland School of Medicine, Baltimore 21201, USA.
authors.forename: V V
authors.initials: VV
authors.lastname: Yurovsky
mesh_terms: D006801:Humans; D011948:Receptors, Antigen, T-Cell / Q000145:classification*; D012595:Scleroderma, Systemic
chemical_list: D011948:Receptors, Antigen, T-Cell