Platelet and coagulation factor variations induced in vitro by polyethylene terephthalate (Dacron) coated with pyrolytic carbon.
The haemocompatibility of polyethylene terephthalate (Dacron) coated with pyrolytic carbon was examined in vitro, evaluating its capability of inducing adhesion and platelet activation, and of modifying the intrinsic coagulation pathway. Platelet adhesion was evaluated by counting platelets before and after in vitro contact of human plasma with the material under examination. Platelet activation was evaluated by determining platelet factor 4 (PF4) and thromboxane B2. Intrinsic coagulation pathway alterations were studied by determining activated partial thromboplastin time (APTT) and the activity of single factors. The results obtained show that pyrolytic carbon-coated Dacron induces platelet adhesion, reduction in platelet volume and lower increase in thromboxane production than that obtained after contact with uncoated Dacron. Pyrolytic carbon-coated Dacron does not induce PF4 release, contrary to uncoated Dacron induces a significant release. Moreover, pyrolytic carbon-coated Dacron, induces a significant extension of APTT by reducing the activity of intrinsic pathway factors, particularly factor XI.
Cenni
E
E
Laboratory for Biocompatibility Research on Implant Materials, Istituti Ortopedici Rizzoli, Bologna, Italy.
Arciola
C R
CR
Ciapetti
G
G
Granchi
D
D
Savarino
L
L
Stea
S
S
Cavedagna
D
D
Curti
T
T
Falsone
G
G
Pizzoferrato
A
A
D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology*; D001792:Blood Platelets / Q000187:drug effects*; D002244:Carbon / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D010314:Partial Thromboplastin Time; D015539:Platelet Activation / Q000187:drug effects; D010973:Platelet Adhesiveness / Q000187:drug effects; D010976:Platelet Count / Q000187:drug effects; D010978:Platelet Factor 4 / Q000032:analysis* / Q000378:metabolism / Q000502:physiology; D011093:Polyethylene Terephthalates / Q000737:chemistry / Q000494:pharmacology*; D013929:Thromboxane B2 / Q000032:analysis
D001672:Biocompatible Materials; D011093:Polyethylene Terephthalates; D010978:Platelet Factor 4; D013929:Thromboxane B2; D002244:Carbon