Physical mapping of SOD1 to bovine chromosome 1.
Superoxide dismutase 1 (SOD1) was mapped to cattle chromosome 1q12 --> q14 by in situ methods. Both traditional in situ hybridization using tritium and a new technique, direct in-situ single copy PCR (DISC-PCR), were used in two separate laboratories. Both human and bovine SOD1 clones were tritium labeled for radioactive in situ hybridization. A primer pair based on the bovine SOD1 gene (Barendse et al., 1994b) was used for the DISC-PCR procedure. The map location of SOD1 is close to collagen 6A1. SOD1 is a potentially important type 1 anchor locus in the region where the gene for horns in cattle was recently mapped (Georges et al., 1993; Schmutz et al., 1995).
Schmutz
S M
SM
Department of Animal and Poultry Science, University of Saskatchewan, Saskatoon, Canada. SCHMUTZ@SASK.USASK.CA
Cornwell
D
D
Moker
J S
JS
Troyer
D L
DL
D000818:Animals; D001483:Base Sequence; D002417:Cattle / Q000235:genetics*; D002874:Chromosome Mapping; D017931:DNA Primers / Q000737:chemistry; D006801:Humans; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D013482:Superoxide Dismutase / Q000235:genetics*
D017931:DNA Primers; D013482:Superoxide Dismutase