title: Changes of erythrocyte ouabain maximum binding after birth in neonates--in relation to erythrocyte sodium and potassium concentrations.
abstract: Erythrocyte ouabain maximum binding (Vmax level) represents the number of molecules of Na-K ATPase on the erythrocyte membrane. Erythrocyte Vmax levels and sodium potassium concentrations were studied in 38 mature and 57 premature infants, including 40 very low birth weight infants, 41 children and 16 adults, in order to clarify the development of erythrocyte Na-K ATPase related to mineral balance and thermogenesis. Vmax levels were the highest (22.9 +/- 8.9 nmol/l cells) for premature infants; correlated negatively with gestational age (r = -0.77, P < 0.01). Vmax levels correlated positively with the ratio of erythrocyte potassium to sodium concentration (IC-K/Na) at < 20 nmol/l cells of Vmax levels (r = 0.72, P < 0.01), but not at > or = 20 nmol/l cells (r = 0.23). Vmax levels in very low birth weight infants, especially < 28 weeks postconception, decreased gradually with postnatal days. Ln(Vmax) levels correlated negatively with postconceptional age (r = -0.75, P < 0.01). Under 28 weeks postconception, Vmax levels were high, but not correlated with IC-K/Na. It is probable that individual Na-K ATPase molecules may be less active, and the large numbers of Na-K ATPase molecules may compensate for the individual lower activity.
authors.affiliation: Department of Pediatrics, Kyoto Prefectural University of Medicine, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Matsuo
authors.forename: F
authors.initials: F
authors.lastname: Inoue
authors.forename: H
authors.initials: H
authors.lastname: Yoshioka
authors.forename: A
authors.initials: A
authors.lastname: Kinugasa
authors.forename: M
authors.initials: M
authors.lastname: Uchiyama
authors.forename: T
authors.initials: T
authors.lastname: Sawada
mesh_terms: D000293:Adolescent; D000328:Adult; D000704:Analysis of Variance; D001724:Birth Weight / Q000502:physiology; D002648:Child; D002675:Child, Preschool; D004910:Erythrocyte Membrane / Q000737:chemistry / Q000201:enzymology; D004912:Erythrocytes / Q000737:chemistry* / Q000378:metabolism; D005260:Female; D006801:Humans; D007223:Infant; D007231:Infant, Newborn / Q000097:blood*; D007234:Infant, Premature / Q000097:blood; D019102:Infant, Very Low Birth Weight / Q000097:blood; D008297:Male; D010042:Ouabain / Q000032:analysis / Q000097:blood*; D011188:Potassium / Q000097:blood*; D012964:Sodium / Q000097:blood*; D000254:Sodium-Potassium-Exchanging ATPase / Q000032:analysis
chemical_list: D010042:Ouabain; D012964:Sodium; D000254:Sodium-Potassium-Exchanging ATPase; D011188:Potassium