Processing of pro-islet amyloid polypeptide (proIAPP) by the prohormone convertase PC2.
Islet amyloid polypeptide (IAPP), 'amylin', is the component peptide of islet amyloid formed in Type 2 diabetes. IAPP is expressed in islet beta-cells and is derived from a larger precursor, proIAPP, by proteolysis. An in vitro translation/translocation system was used to separately examine processing of human proIAPP by the beta-cell endopeptidases PC2, PC3 or furin. ProIAPP was converted to mature IAPP by PC2 but there was little conversion by furin or PC3. These data are consistent with processing of proIAPP in beta-cell secretory granules. Abnormal cellular proteolysis associated with type 2 diabetes could contribute to IAPP amyloidosis.
Badman
M K
MK
Department of Human Anatomy, University of Oxford, UK.
Shennan
K I
KI
Jermany
J L
JL
Docherty
K
K
Clark
A
A
D000595:Amino Acid Sequence; D000682:Amyloid / Q000235:genetics* / Q000378:metabolism*; D000818:Animals; D016282:Aspartic Acid Endopeptidases / Q000235:genetics / Q000378:metabolism; D045683:Furin; D006801:Humans; D058228:Islet Amyloid Polypeptide; D008969:Molecular Sequence Data; D045706:Proprotein Convertase 2; D043484:Proprotein Convertases; D014176:Protein Biosynthesis; D011498:Protein Precursors / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger; D013381:Subtilisins / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic; D014981:Xenopus
D000682:Amyloid; D058228:Islet Amyloid Polypeptide; D011498:Protein Precursors; D012333:RNA, Messenger; D043484:Proprotein Convertases; D013381:Subtilisins; D045683:Furin; D045706:Proprotein Convertase 2; D016282:Aspartic Acid Endopeptidases