title: Complete development of Cryptosporidium parvum in bovine fallopian tube epithelial cells.
abstract: Cryptosporidium parvum is a coccidian parasite responsible for causing protracted and life-threatening diarrheal illness in immunocompromised humans, especially patients with AIDS. The lack of medications effective in treating people suffering from cryptosporidiosis has prompted the development of in vivo and in vitro models for this disease. This study is the first to demonstrate that C. parvum can complete its entire life cycle (from sporozoite to infective oocyst) in a primary culture of bovine fallopian tube epithelial (BFTE) cells. Scanning and transmission electron photomicrographs were used to detail the ultrastructure of individual parasitic stages. Successful infections were produced by inoculating cell cultures with either oocysts or purified sporozoites. Infection of BFTE cells with C. parvum close paralleled in vivo infections with regard to host cell location and chronology of parasite development. Infecting BFTE cells with sporulated oocysts provided a reproducible and quantitative cultivation system with significantly (P < or = 0.001) higher infection rates than in Madin-Darby canine kidney cells. Oocysts produced in BFTE cells were infective for immunosuppressed adult C57BL/6N mice. Cultivation of C. parvum in BFTE cells will facilitate the study of interactions between parasites and host cells as well as provide a reliable system for evaluating anticryptosporidial compound efficacy.
authors.affiliation: Department of Animal, Dairy and Veterinary Sciences, Utah State University, Logan 84322-5600, USA.
authors.forename: S
authors.initials: S
authors.lastname: Yang
authors.forename: M C
authors.initials: MC
authors.lastname: Healey
authors.forename: C
authors.initials: C
authors.lastname: Du
authors.forename: J
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D002675:Child, Preschool; D016785:Cryptosporidium parvum / Q000254:growth & development* / Q000648:ultrastructure; D004848:Epithelium / Q000469:parasitology; D005187:Fallopian Tubes / Q000166:cytology / Q000469:parasitology*; D005260:Female; D006801:Humans; D007668:Kidney / Q000166:cytology / Q000469:parasitology