Expression of endogenous muscarinic acetylcholine receptors in Chinese hamster ovary cells.
Chinese hamster ovary (CHO) cells are commonly used for expression of the genes of cloned neurotransmitter receptors to study their pharmacology and coupling to signal transduction pathways. It is usually assumed that host cells do not endogenously express the specific receptor under consideration. We demonstrate in this report that CHO cells contain endogenous functional muscarinic acetylcholine receptors which, in some circumstances, might complicate interpretation of data related to the properties of exogenously expressed receptors.
Wang
S Z
SZ
Division of Neuroscience Research in Psychiatry, University of Minnesota, Minneapolis 55455, USA.
Zhu
S Z
SZ
el-Fakahany
E E
EE
D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D006152:Cyclic GMP / Q000096:biosynthesis; D004305:Dose-Response Relationship, Drug; D015870:Gene Expression; D011950:Receptors, Cholinergic / Q000378:metabolism*; D011976:Receptors, Muscarinic / Q000378:metabolism*
D011950:Receptors, Cholinergic; D011976:Receptors, Muscarinic; D006152:Cyclic GMP