title: Abnormalities in protein kinase C and MAP kinase cascade in mesangial cells cultured under high glucose conditions.
abstract: In order to clarify the mechanism of mesangial cell dysfunction in diabetes, we examined the activities of protein kinase C (PKC) and mitogen-activated protein kinase (MAPK), important kinases in various cellular functions, and also evaluated the isoenzymes of PKC in mesangial cells cultured under high glucose conditions. Exposure of cells to high concentrations (27.8 mM) of glucose for 5 days resulted in a significant elevation of PKC activities in the membrane fraction. MAPK was also activated in cells cultured under high glucose conditions. Of the PKC isoenzymes, the levels of PKC alpha and zeta were significantly increased in the membrane fraction after 5 days of exposure to high concentrations of glucose. These results indicate that the translocation of PKC alpha and zeta and the activation of MAPK under high glucose conditions might be underlying mechanisms of the functional disturbance of mesangial cells in diabetes.
authors.affiliation: Third Department of Medicine, Shiga University of Medical Science, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Haneda
authors.forename: R
authors.initials: R
authors.lastname: Kikkawa
authors.forename: T
authors.initials: T
authors.lastname: Sugimoto
authors.forename: D
authors.initials: D
authors.lastname: Koya
authors.forename: S
authors.initials: S
authors.lastname: Araki
authors.forename: M
authors.initials: M
authors.lastname: Togawa
authors.forename: Y
authors.initials: Y
authors.lastname: Shigeta
mesh_terms: D000818:Animals; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000378:metabolism*; D002478:Cells, Cultured; D004789:Enzyme Activation; D005920:Glomerular Mesangium / Q000187:drug effects / Q000201:enzymology*; D005947:Glucose / Q000494:pharmacology*; D015151:Immunoblotting; D007527:Isoenzymes / Q000032:analysis / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D011493:Protein Kinase C / Q000032:analysis / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
chemical_list: D007527:Isoenzymes; D011493:Protein Kinase C; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D005947:Glucose