GUF1, a gene encoding a novel evolutionarily conserved GTPase in budding yeast.
While sequencing a region of chromosome IV adjacent to the checkpoint gene MEC3, we identified a gene we call GUF1 (GTPase of Unknown Function), which predicts a 586 amino acid GTPase of the elongation factor-type class. The predicted Guf1p protein bears striking sequence similarity to both LepA from Escherichia coli (43% identical) and LK1236.1 from Caenorhabditis elegans (42% identical). Analysis of both a guf1 delta deletion and a putative constitutive-activating mutant (GUF1HG) revealed that GUF1 is not essential nor did mutant cells reveal any marked phenotype.
Kiser
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Molecular and Cellular Biology Department, University of Arizona, Tucson 85721, USA.
Weinert
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TA
D000595:Amino Acid Sequence; D001483:Base Sequence; D005075:Biological Evolution; D017124:Conserved Sequence; D020558:GTP Phosphohydrolases / Q000235:genetics*; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*
D020558:GTP Phosphohydrolases