title: Molecular subtyping of prevalent M serotypes of Streptococcus pyogenes causing invasive disease.
abstract: Reproducible methodologies and a scheme for high-resolution genotyping of Streptococcus pyogenes were defined with respect to a study of six predominant M serotypes causing invasive group A streptococcal disease in the United Kingdom. Serotype reference strains were compared with nine clinical isolates of each serotype from patients with diseases such as pneumonia, puerperal sepsis, toxic shock-like-syndrome, cellulitis, or necrotizing fasciitis. Four enzymes were evaluated for their discriminatory power in 16S rRNA gene-specific ribotyping. Discriminatory power was greatest with EcoRI, which generated serotype-specific ribotypes, and with SacI, which could subdivide strains of the same M serotype. Twenty-five combined ribotypes were found among the 60 strains, and the indices of discriminatory power (D values) of this method varied from 0.51 within serotype M1 to 0.98 within strains of serotype M5. Macrorestriction with the rarely cutting endonuclease SmaI and pulsed-field gel electrophoresis gave D values varying from 0.37 within serotype M1 to the maximal 1.0 within serotype M5. Comparison of macrorestriction profiles revealed various degrees of genetic heterogeneity within M serotypes. Strains of M1, M3, M6, and M11 exhibited clonally related macrorestriction profiles, while those of R28 and M5 strains were consistent with polyphyletic origin.
authors.affiliation: Molecular Biology Unit, Central Public Health Laboratory, London, United Kingdom.
authors.forename: J
authors.initials: J
authors.lastname: Stanley
authors.forename: D
authors.initials: D
authors.lastname: Linton
authors.forename: M
authors.initials: M
authors.lastname: Desai
authors.forename: A
authors.initials: A
authors.lastname: Efstratiou
authors.forename: R
authors.initials: R
authors.lastname: George
mesh_terms: D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins / Q000276:immunology; D015373:Bacterial Typing Techniques; D001483:Base Sequence; D002352:Carrier Proteins; D004275:DNA, Ribosomal / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D005819:Genetic Markers; D014644:Genetic Variation; D006801:Humans; D015336:Molecular Probe Techniques; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012703:Serotyping; D013290:Streptococcal Infections / Q000453:epidemiology / Q000276:immunology / Q000382:microbiology*; D013297:Streptococcus pyogenes / Q000145:classification* / Q000235:genetics / Q000276:immunology; D006113:United Kingdom / Q000453:epidemiology
chemical_list: D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D002352:Carrier Proteins; D004275:DNA, Ribosomal; D005819:Genetic Markers; D012336:RNA, Ribosomal, 16S; C014689:streptococcal M protein