title: Characterisation of a short interspersed repeat (Mermaid) that has family members on human chromosome 21 and elsewhere in the human genome.
abstract: To understand the architecture of the human genome, we need a complete definition of all the repeat sequence families, as these make up the majority of human DNA. We have isolated a small DNA fragment from human chromosome 21 and have used sequence analysis of this fragment to uncover a new low copy repeat element of approximately 300 bp that we term the Mermaid repeat. This repeat is related to, but is different from, the MER12 repeat and is interspersed in the genome. Mermaid family members that we have studied are between 81%-87% identical to our preliminary consensus sequence. Therefore, we have added a new member to the large collection of human repetitive elements. In addition, we have mapped a Mermaid repeat to a telomeric position on the long arm of human chromosome 21, at 21q22.3.
authors.affiliation: Department of Biochemistry and Molecular Genetics, St. Mary's Hospital Medical School, Imperial College, London, UK.
authors.forename: J
authors.initials: J
authors.lastname: Hoyle
authors.forename: I G
authors.initials: IG
authors.lastname: Yulug
authors.forename: K
authors.initials: K
authors.lastname: Johnstone
authors.forename: P J
authors.initials: PJ
authors.lastname: Scambler
authors.forename: E M
authors.initials: EM
authors.lastname: Fisher
mesh_terms: D001483:Base Sequence; D015139:Blotting, Southern; D002874:Chromosome Mapping; D002891:Chromosomes, Human, Pair 21; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D015342:DNA Probes; D018076:DNA, Complementary; D015894:Genome, Human; D006801:Humans; D008969:Molecular Sequence Data; D012091:Repetitive Sequences, Nucleic Acid; D012689:Sequence Homology, Nucleic Acid
chemical_list: D015342:DNA Probes; D018076:DNA, Complementary; D004247:DNA