Regulation of anti-SRBC antibody production by opioids and their mechanisms.
This study focused on the influences of opioids on the generation of antibody against sheep erythrocyte in vitro. It was found that morphine, a-CAO, DADLE, MENK were able to inhibit the capacity of murine spleen cells to generate antibody and leukotriene C4 and conversely, dynorphin was able to stimulate the capacity of murine spleen cells to generate antibody and leukotriene C4. Morphine, a-CAO, MENK, DADLE, dynorphin decreased intracellular cAMP level, increased [Ca2+]i and calmodulin activity. The effects were completely blocked by naloxone, the specific opioid antagonist. Our results showed that opioids regulate the production of antibody in murine spleen cells, and alter intracellular cAMP, [Ca2+]i calmodulin activity, and leukotriene C4 production by way of binding to different receptor types.
Wang
H
H
Dept. of Pharmacology, CAMS, Beijing.
Lin
J
J
Liu
J
J
D000818:Animals; D000917:Antibody Formation / Q000187:drug effects*; D002118:Calcium / Q000097:blood; D002147:Calmodulin / Q000097:blood; D000242:Cyclic AMP / Q000097:blood; D004399:Dynorphins / Q000494:pharmacology; D004912:Erythrocytes / Q000276:immunology*; D005260:Female; D006168:Guinea Pigs; D017997:Leukotriene C4 / Q000096:biosynthesis; D008214:Lymphocytes / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009020:Morphine / Q000494:pharmacology; D009294:Narcotics / Q000494:pharmacology*; D017473:Receptors, Opioid, kappa / Q000819:agonists; D012756:Sheep
D002147:Calmodulin; D009294:Narcotics; D017473:Receptors, Opioid, kappa; D017997:Leukotriene C4; D004399:Dynorphins; D009020:Morphine; D000242:Cyclic AMP; D002118:Calcium