Cytosolic ascorbate peroxidase from Arabidopsis thaliana L. is encoded by a small multigene family.
A second cytosolic ascorbate peroxidase (cAPX; EC 1.11.1.11) gene from Arabidopsis thaliana has been characterised. This second gene (designated APX1b) maps to linkage group 3 and potentially encodes a cAPX as closely related to that from other dicotyledonous species as to the other member of this gene family (Kubo et al., 1993, FEBS Lett 315: 313 317; here designated APX1a), which maps to linkage group 1. In contrast, the lack of sequence similarity in non-coding regions of the genes implies that they are differentially regulated. Under non-stressed conditions only APX1a is expressed. APX1b was identified during low-stringency probing using a cDNA coding for pea cAPX which, in turn, was recovered from a cDNA library by immunoscreening with an antiserum raised against tea plastidial APX (pAPX). No pAPX cDNAs were recovered, despite the antiserum displaying specificity for pAPX in Western blots.
Santos
M
M
John Innes Centre, Colney, UK.
Gousseau
H
H
Lister
C
C
Foyer
C
C
Creissen
G
G
Mullineaux
P
P
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics*; D029681:Arabidopsis Proteins; D060387:Ascorbate Peroxidases; D001483:Base Sequence; D003600:Cytosol / Q000201:enzymology; D017931:DNA Primers; D018076:DNA, Complementary; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D015698:Genomic Library; D007527:Isoenzymes / Q000096:biosynthesis / Q000235:genetics; D008969:Molecular Sequence Data; D005810:Multigene Family; D010544:Peroxidases / Q000096:biosynthesis / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D014675:Vegetables / Q000201:enzymology
D029681:Arabidopsis Proteins; D017931:DNA Primers; D018076:DNA, Complementary; D007527:Isoenzymes; D010544:Peroxidases; C555293:APX2 protein, Arabidopsis; D060387:Ascorbate Peroxidases