Effect of 1,10-phenanthroline on DNA binding by 1,25-(OH)2 vitamin D receptor.
The effect of the chelator 1,10-phenanthroline on the DNA-binding of 1,25-dihydroxyvitamin D receptor was demonstrated. Increasing concentrations of the chelator, up to 3 mM, decreased receptor binding to DNA-cellulose. This effect was specific for the chelating function because the nonchelating analog, 1,7-phenanthroline, did not inhibit DNA-cellulose binding of the receptor. The cations Cd2+ and Zn2+ were able to block chelator-mediated inhibition. These cations were unable to reverse the inhibition unless 1,10-phenanthroline was removed by dialysis.
Atkins
K B
KB
Department of Animal Science, Iowa State University, Ames, USA.
Beitz
D C
DC
D000818:Animals; D002104:Cadmium / Q000494:pharmacology; D002412:Cations / Q000494:pharmacology; D002614:Chelating Agents / Q000494:pharmacology*; D002850:Chromatography, Gel; D004247:DNA / Q000378:metabolism*; D003956:Dialysis; D004492:Edetic Acid / Q000494:pharmacology; D008297:Male; D010618:Phenanthrolines / Q000494:pharmacology*; D011485:Protein Binding; D051381:Rats; D017208:Rats, Wistar; D018167:Receptors, Calcitriol / Q000378:metabolism*; D015032:Zinc / Q000494:pharmacology
D002412:Cations; D002614:Chelating Agents; D010618:Phenanthrolines; D018167:Receptors, Calcitriol; D002104:Cadmium; C001635:ferroin; D004247:DNA; D004492:Edetic Acid; D015032:Zinc