Regulation of lineage-specific transcription of the sucrase-isomaltase gene in transgenic mice and cell lines.
Sucrase-isomaltase (SI), a gene expressed exclusively in absorptive enterocytes, was used to examine the molecular mechanisms that regulate cell-specific gene expression in the intestinal epithelium. Transgenic mice were made with a construct containing nucleotides -8,500 to +54 of the mouse SI gene linked to a human growth hormone reporter gene. In adult transgenic animals, high-level transgene expression was limited to the small intestine, with low levels of ectopic expression in the colon. In contrast to the endogenous gene that is expressed only in enterocytes, the transgene was expressed in all four cell lineages, including enterocytes, enteroendocrine, goblet, and Paneth cells. To examine this process of lineage-specific expression further we studied Caco-2 and COLO DM cell lines, which model enterocytes and enteroendocrine cells, respectively. Reminiscent of results in transgenic animals, only Caco-2 cells transcribed the endogenous SI gene, whereas both Caco-2 and COLO DM cells supported transcription from chimeric SI reporter gene constructs. Taken together, these data suggest that each intestinal cell lineage has the cellular machinery to transcribe the SI gene. Moreover, these findings imply that transcription is normally repressed in nonenterocytic cells, possibly via a transcriptional silencer residing outside of the region of the SI gene examined in these studies.
Markowitz
A J
AJ
Department of Medicine and Genetics, University of Pennsylvania School of Medicine, Philadelphia 19104-6144, USA.
Wu
G D
GD
Bader
A
A
Cui
Z
Z
Chen
L
L
Traber
P G
PG
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003106:Colon / Q000502:physiology; D004702:Endocrine Glands / Q000166:cytology / Q000502:physiology; D005796:Genes; D013006:Growth Hormone / Q000235:genetics; D006801:Humans; D007413:Intestinal Mucosa / Q000166:cytology / Q000502:physiology; D051379:Mice; D008822:Mice, Transgenic; D015335:Molecular Probes / Q000235:genetics; D008969:Molecular Sequence Data; D009834:Oligo-1,6-Glucosidase / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism; D013393:Sucrase / Q000235:genetics*; D014158:Transcription, Genetic
D015335:Molecular Probes; D012333:RNA, Messenger; D013006:Growth Hormone; D009834:Oligo-1,6-Glucosidase; D013393:Sucrase