Monoclonal antibody markers for early development of the stereociliary bundles of mammalian hair cells.
Two monoclonal antibodies, SC1 and SC2, were raised in vitro against antigens from the stereocilia of guinea-pig hair cells. They both labelled stereociliary antigens that were not detected in any other cell within the cochlear duct or the vestibular epithelial. SC1 cross-reacted with the tectorial membrane in the cochlea and labelled both cochlear and vestibular hair cells from both the mouse and the rat. In the mouse the SC1 antigen was labelled from embryonic days 16-18, coincident with the development of the stereociliary bundles. SC1 cross-reacted with neuromuscular junctions from striated muscle and with basal keratinocytes in skin. SC2 did not cross-react cleanly with hair cells from the mouse or the rat but it cross-reacted with proximal tubules of the guinea-pig kidney. Both antibodies can be used as cellular markers within the guinea-pig cochlea and SC1 should be particularly useful for studies of hair cell differentiation in the mouse.
Holley
M C
MC
Department of Physiology, School of Medical Sciences, University Walk, Bristol, UK.
Nishida
Y
Y
D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D000941:Antigens / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006168:Guinea Pigs; D006198:Hair Cells, Auditory / Q000254:growth & development* / Q000276:immunology / Q000648:ultrastructure*; D006825:Hybridomas / Q000276:immunology; D051379:Mice; D017830:Octoxynol; D009924:Organ Culture Techniques; D051381:Rats; D013045:Species Specificity; D014357:Trypsin / Q000378:metabolism
D000911:Antibodies, Monoclonal; D000941:Antigens; D017830:Octoxynol; D014357:Trypsin