title: Immunohistochemical identification of proteoglycan form of macrophage colony-stimulating factor on bone surface.
abstract: Several studies using op/op mice have shown that macrophage colony-stimulating factor (M-CSF) was necessary for osteoclast formation in vivo. Previously we reported that osteoblastic cells produced two molecular forms of M-CSF; one is an 85-kDa M-CSF, and the other is a proteoglycan form of M-CSF (PG-M-CSF) which has a binding affinity for bone-derived collagens and is extractable from human bone. In this study, we performed immunostaining of human bone using a newly established anti-PG-M-CSF antibody, and showed positive staining PG-M-CSF, probably produced by bone lining cells, on the bone surface. This observation suggests that the bone surface is suitable for osteoclast formation because of the presence of PG-M-CSF.
authors.affiliation: Department of Internal Medicine, Jichi Medical School, Tochigi, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Ohtsuki
authors.forename: K
authors.initials: K
authors.lastname: Hatake
authors.forename: S
authors.initials: S
authors.lastname: Suzu
authors.forename: K
authors.initials: K
authors.lastname: Saito
authors.forename: K
authors.initials: K
authors.lastname: Motoyoshi
authors.forename: Y
authors.initials: Y
authors.lastname: Miura
mesh_terms: D000368:Aged; D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D001842:Bone and Bones / Q000378:metabolism*; D016466:CHO Cells; D006224:Cricetinae; D006801:Humans; D007150:Immunohistochemistry; D016173:Macrophage Colony-Stimulating Factor / Q000276:immunology / Q000378:metabolism*; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D011509:Proteoglycans / Q000378:metabolism*; D011817:Rabbits
chemical_list: D000911:Antibodies, Monoclonal; D011509:Proteoglycans; D016173:Macrophage Colony-Stimulating Factor