The tuberous sclerosis 2 gene is expressed at high levels in the cerebellum and developing spinal cord.
Tuberous sclerosis (TS) is an autosomal dominant multisystem disorder characterized by the widespread development of hamartomas in many tissues and organs. TSC2 is predicted to encode a 1784-amino acid tumor suppressor protein that may function, in part, as a GTPase-activating protein for Rap1. Given the high incidence of central nervous system abnormalities in individuals affected with tuberous sclerosis, the expression of TSC2 in developing and adult nervous system tissues was examined. Reverse transcription-PCR, Northern blot, and in situ hybridization analyses demonstrated high levels of expression of TSC2 in the adult brain and developing central nervous system. Abundant TSC2 expression was detected in the adult cerebellum, hippocampus, and olfactory bulb, with lower levels of expression observed in other tissues, including heart and kidney. This enrichment of TSC2 expression in neurons in the central nervous system suggests unique roles for this tumor suppressor gene product in the development and differentiation of nervous system tissues.
Geist
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RT
Department of Neurology, Washington University School of Medicine, St. Louis, Missouri 63110, USA.
Gutmann
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D000328:Adult; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002531:Cerebellum / Q000502:physiology*; D019204:GTP-Binding Proteins / Q000648:ultrastructure; D020690:GTPase-Activating Proteins; D015870:Gene Expression / Q000502:physiology; D018507:Gene Expression Regulation, Developmental / Q000502:physiology; D006801:Humans; D017403:In Situ Hybridization; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011506:Proteins / Q000648:ultrastructure; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D013116:Spinal Cord / Q000196:embryology / Q000502:physiology*; D014157:Transcription Factors / Q000648:ultrastructure; D014402:Tuberous Sclerosis / Q000235:genetics*; D020668:rap GTP-Binding Proteins; D020703:ras GTPase-Activating Proteins
D020690:GTPase-Activating Proteins; D011506:Proteins; D012333:RNA, Messenger; D014157:Transcription Factors; D020703:ras GTPase-Activating Proteins; D019204:GTP-Binding Proteins; D020668:rap GTP-Binding Proteins