title: GUF1, a gene encoding a novel evolutionarily conserved GTPase in budding yeast.
abstract: While sequencing a region of chromosome IV adjacent to the checkpoint gene MEC3, we identified a gene we call GUF1 (GTPase of Unknown Function), which predicts a 586 amino acid GTPase of the elongation factor-type class. The predicted Guf1p protein bears striking sequence similarity to both LepA from Escherichia coli (43% identical) and LK1236.1 from Caenorhabditis elegans (42% identical). Analysis of both a guf1 delta deletion and a putative constitutive-activating mutant (GUF1HG) revealed that GUF1 is not essential nor did mutant cells reveal any marked phenotype.
authors.affiliation: Molecular and Cellular Biology Department, University of Arizona, Tucson 85721, USA.
authors.forename: G L
authors.initials: GL
authors.lastname: Kiser
authors.forename: T A
authors.initials: TA
authors.lastname: Weinert
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D005075:Biological Evolution; D017124:Conserved Sequence; D020558:GTP Phosphohydrolases / Q000235:genetics*; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*
chemical_list: D020558:GTP Phosphohydrolases