Kinetic mechanism of pyrophosphate-dependent phosphofructokinase from Giardia lamblia.
The steady-state kinetics of the reaction catalyzed by inorganic-pyrophosphate-dependent D-fructose-6-phosphate 1-phosphotransferase from Giardia lamblia have been investigated. The reactants for the forward and reverse reactions were the Mg-chelated complexes of pyrophosphate (PPi) and Pi. Uncomplexed ligands were not substrates. In the direction of phosphorylation of fructose-6-phosphate (F6P), initial velocity double-reciprocal plots for both PPi and F6P were intersecting suggesting sequential addition of substrates. Similarly, intersecting patterns were observed in the reverse reaction with either Pi or fructose-1,6-bisphosphate (FBP) as the variable substrate. Although the catalytic constants for the forward and reverse reactions were found to be identical (83 s-1), the kcat/Km for PPi is about two orders of magnitude higher than the kcat/Km for Pi, indicating that PPi is utilized much more efficiently than Pi. Product inhibition of Pi is competitive vs. PPi and noncompetitive vs. F6P, when the fixed substrate is subsaturating. Product inhibition by FBP was found to be noncompetitive with either Pi or F6P as the variable substrate. These results are consistent with a sequential ordered Bi Bi mechanism with PPi adding first and Pi dissociating last. In the reverse reaction, however, PPi and F6P were found to be noncompetitive with either Pi or FBP. Dead-end inhibition analysis with fructose 2,6-bisphosphate, a competitive substrate analog of FBP, gave uncompetitive inhibition with respect to Pi, indicating that fructose 2,6-bisphosphate (and hence FBP) binds after Pi. This kinetic mechanism is different from that observed with the enzyme from Propionibacterium freudenreichii, Entamoeba histolytica or Mung bean, which were concluded to be rapid equilibrium random mechanism.
Phillips
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NF
Department of Biochemistry and Medicine, Case Western Reserve University, School of Medicine, Cleveland, OH 44106-4983, USA.
Li
Z
Z
D000818:Animals; D001667:Binding, Competitive; D002614:Chelating Agents; D011756:Diphosphates / Q000378:metabolism; D005635:Fructosediphosphates / Q000378:metabolism; D005636:Fructosephosphates / Q000378:metabolism; D016829:Giardia lamblia / Q000201:enzymology*; D007700:Kinetics; D008274:Magnesium; D010770:Phosphotransferases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D013379:Substrate Specificity
D002614:Chelating Agents; D011756:Diphosphates; D005635:Fructosediphosphates; D005636:Fructosephosphates; C027618:fructose-6-phosphate; C027652:fructose 2,6-diphosphate; D010770:Phosphotransferases; C022502:pyrophosphate-fructose 6-phosphate 1-phosphotransferase; D008274:Magnesium; C029063:fructose-1,6-diphosphate