[Cloning and overexpression of rat ovary LH/hCG receptor cDNA in insect cells].
Luteinizing hormone/human chorionic gonadotropin receptor (LH/hCG receptor) is a glycosylated protein coupled with G-protein. This paper reports screening of LH/hCG receptor cDNA from rat ovary cDNA library and the overexpression of the cDNA in insect cells. Full length of LH/hCG receptor cDNA is 2403 bp encoding signal peptide and matured protein of LH/hCG receptor. The cDNA is overexpressed in insect cells using baculovirus expression vector pVL 1393. The apparent molecular weight of purified receptor using immunoaffinity chromatography is 120 Kd and 92 Kd under non-reducing and reducing conditions, respectively. Ligand binding assay and Scatchard Plot analysis indicates that Kd is 8.4 x 10(-9) mol/L which is similar to that of the purified receptor from CHO cells.
Shen
Q X
QX
Shanghai Institute of Cell Biology, Academia Sinica.
Liu
H H
HH
Chen
W Y
WY
Bahl
O P
OP
D000818:Animals; D016367:Baculoviridae / Q000235:genetics*; D001483:Base Sequence; D002478:Cells, Cultured; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism*; D005260:Female; D005822:Genetic Vectors / Q000235:genetics*; D007313:Insecta / Q000166:cytology / Q000378:metabolism*; D008969:Molecular Sequence Data; D010053:Ovary / Q000737:chemistry; D051381:Rats; D011974:Receptors, LH / Q000235:genetics*
D018076:DNA, Complementary; D011974:Receptors, LH