The stimulatory effect on human erythrocyte rubidium-86 uptake by anti-cardiac-glycoside antibodies.
Considerable, but as yet still controversial evidence indicates the presence, in mammalian tissues of endogenous digitalis-like factors (EDLFs) which inhibit cell membrane Na+, K(+)-adenosine triphosphatase (Na+, K(+)-ATPase) and which may cross-react with anti-digitalis antibodies. The aim of this study was to evaluate the effect of antibodies against cardiac glycosides on Na+, K(+)-ATPase in human erythrocytes. For this purpose, we measured the effect of antibodies against two different cardiac glycosides (anti-ouabain rabbit antiserum and anti-digoxin Fab fragments) on the activity of the Na+, K(+)-ATPase, as measured by erythrocyte rubidium-86 (86Rb) uptake, in subjects who had never come into contact with exogenous cardiac glycosides, and compared these results with the effect of two control rabbit sera: a normal serum and an antiserum to a non-related antigen. Anti-ouabain rabbit antiserum and anti-digoxin Fab fragments induced a significantly greater percentage change in 86Rb uptake in the erythrocytes than the two control sera (ANOVA followed by multiple comparison by the Games-Howell test). The average percentage change was +11.8 +/- 16.3% (n = 19) (mean +/- SD) for anti-ouabain antiserum +10.8 +/- 15.6% (n = 23) for anti-digoxin Fab fragments, -1.68 +/- 11.2% (n = 11) for anti-rhGM-CSF antiserum, and -5.8 +/- 11.7 (n = 10) for normal control serum. In a subgroup of ten subjects in whom the 3 antisera were tested simultaneously, the stimulation of erythrocyte 86Rb uptake induced by the two antidigitalis antibodies correlated significantly (r = 0.906, p = 0.001, n = 10).(ABSTRACT TRUNCATED AT 250 WORDS)
Balzan
S
S
CNR Institute of Clinical Physiology, Pisa, Italy.
Montali
U
U
Pieraccini
L
L
Di Bartolo
V
V
Pegoraro
S
S
Revoltella
R
R
Ghione
S
S
D000328:Adult; D000368:Aged; D000818:Animals; D000906:Antibodies / Q000494:pharmacology*; D002301:Cardiac Glycosides / Q000276:immunology*; D004077:Digoxin / Q000276:immunology; D004912:Erythrocytes / Q000000981:diagnostic imaging / Q000201:enzymology*; D005260:Female; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000276:immunology; D008297:Male; D008875:Middle Aged; D010042:Ouabain / Q000276:immunology; D011817:Rabbits; D011877:Radionuclide Imaging; D012414:Rubidium Radioisotopes; D000254:Sodium-Potassium-Exchanging ATPase / Q000097:blood*
D000906:Antibodies; D002301:Cardiac Glycosides; D007140:Immunoglobulin Fab Fragments; D012414:Rubidium Radioisotopes; C050199:digoxin antibodies Fab fragments; D010042:Ouabain; D004077:Digoxin; D000254:Sodium-Potassium-Exchanging ATPase