Effects of IL-11 on the growth of intestinal epithelial cells in vitro.
The network of interacting factors that control proliferation in the intestinal epithelium is largely unknown. Recently, IL-11 was found to protect animals from lethal doses of cytotoxic agents. Part of this protective action was ascribed to a reduced level of damage in the intestinal epithelium. Whether this was due to a direct effect on epithelial cell cycle progression was unclear. We have addressed this question in vitro and found that IL-11 reversibly inhibited proliferation in untransformed small intestinal IEC18 cells. However, IL-11 did not inhibit transformed SW620 or HT29 colonic cell lines. IL-6 behaved in a similar manner to IL-11. Thus, these results suggest that IL-11 may be an ideal therapy adjuvant, protecting normal cells and further, these results suggest that IL-11 may be involved in the normal growth controls in the intestinal epithelium. The inhibitory response evoked by IL-11 is lost during carcinogenic transformation.
Booth
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CRC Department of Epithelial Biology, Paterson Institute for Cancer Research, Christie Hospital (NHS) Trust, Withington, Manchester, UK.
Potten
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D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line / Q000166:cytology / Q000276:immunology; D002471:Cell Transformation, Neoplastic; D003106:Colon / Q000166:cytology* / Q000276:immunology; D004305:Dose-Response Relationship, Drug; D004847:Epithelial Cells; D004848:Epithelium / Q000276:immunology; D017370:Interleukin-11 / Q000494:pharmacology*; D015850:Interleukin-6 / Q000494:pharmacology; D007422:Intestines / Q000166:cytology* / Q000276:immunology; D051381:Rats; D016212:Transforming Growth Factor beta / Q000494:pharmacology
D017370:Interleukin-11; D015850:Interleukin-6; D016212:Transforming Growth Factor beta