Interaction of nickel with mutagens in the induction of sister chromatid exchanges in human lymphocytes.
In this study, individual treatments of human lymphocytes with Ni(II) [0.5-25 microM], Cr(VI) [0.65-1.30 microM], UV-light or X-rays induced SCEs in a dose-dependent fashion, and combined treatments of Ni(II) with Cr(VI), UV-light or X-rays interacted antagonistically. Nickel, at environmentally relevant exposure levels, can have the effect in complex mixtures of reducing an otherwise positive SCE response and could lead to underestimating human exposures to certain classes of chemicals or radiation. Furthermore, our data indicate that antagonism may occur when human lymphocytes are exposed simultaneously to Ni(II) and Cr(VI), suggesting an explanation for epidemiological studies reporting conflicting results for cytogenetic effects in lymphocytes of workers exposed to chromium and nickel.
Katsifis
S P
SP
Institute of Environmental Medicine, New York University Medical Center, NY 10016, USA.
Kinney
P L
PL
Hosselet
S
S
Burns
F J
FJ
Christie
N T
NT
D002478:Cells, Cultured; D003300:Copper / Q000633:toxicity; D004347:Drug Interactions; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects* / Q000528:radiation effects / Q000648:ultrastructure; D009153:Mutagens / Q000633:toxicity*; D009532:Nickel / Q000633:toxicity*; D012854:Sister Chromatid Exchange / Q000187:drug effects* / Q000528:radiation effects; D014466:Ultraviolet Rays; D014965:X-Rays
D009153:Mutagens; D003300:Copper; D009532:Nickel