title: Re-endothelialisation in autogenous vein grafts.
abstract: OBJECTIVE
  To clarify the course of re-endothelialisation (Re-E) in an entire graft and to establish the effect of immersion media for the preservation of endothelial cells.
  
  
  METHODS
  Autogenous femoral veins of dogs were immersed in heparinised saline solution (n = 18) or heparinized autogenous blood (n = 18). After immersion, the grafts were implanted into bilateral femoral artery, and were retrieved 1 day to 4-8 weeks after implantation.
  
  
  RESULTS
  For the grafts immersed in the heparinised saline solution, the values for % area of endothelial cell coverage before implantation, and at 1 day, 1 week, and 4 weeks after implantation were 44.9%, 6.2%, 14.5%, and 81.3%, respectively. For the grafts immersed in heparinised autogenous blood, the values were 73.5%, 20.6%, 79.2% and 95.5%, respectively. However, such relatively rapid speed of Re-E slowed down considerably after 1 week following implantation in this group.
  
  
  CONCLUSIONS
  The use of heparinized autogenous blood is strongly recommended as a preparation media for autogenous vein grafts. Almost all of the endothelial cells fall away in the earlier period after implantation and regenerate multifocally and irregularly. Re-E is incomplete even at 8 weeks after surgery, and we suggest that the area of incomplete Re-E may develop into intimal hyperplasia.
authors.affiliation: First Department of Surgery, Asahikawa Medical College, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Ishikawa
authors.forename: T
authors.initials: T
authors.lastname: Sasajima
authors.forename: Y
authors.initials: Y
authors.lastname: Kubo
mesh_terms: D000818:Animals; D001769:Blood; D002455:Cell Division; D004285:Dogs; D004730:Endothelium, Vascular / Q000166:cytology*; D005263:Femoral Artery / Q000601:surgery; D005268:Femoral Vein / Q000166:cytology / Q000637:transplantation*; D006493:Heparin / Q000494:pharmacology; D012965:Sodium Chloride; D014182:Transplantation, Autologous
chemical_list: D012965:Sodium Chloride; D006493:Heparin