[In vitro study of the metabolic conversion of aflatoxin B1 into its epoxide].
The conversion of aflatoxin B1 into its epoxide was evaluated in vitro by two different approaches based on HPLC analysis: the quantitative estimation of the tris(OH)AFB1 addition product resulting from the indirect reaction of AFB1-epoxide with tris buffer (hydroxymethyl) aminomethane and on the other hand by the quantification of the formation of the glutathione conjugate (AFB1-SG). The tris(OH)AFB1 is more sensitive than AFB1-SG in fluorescence detection. The AFB1-SG obtained (5.42 +/- 0.42 microgram) is weakly less than the quantity of tris(OH)AFB1 (6.00 +/- 0.72) obtained in the same experimental conditions.
Mukendi
N
N
de Meester
C
C
Rollman
B
B
D016604:Aflatoxin B1 / Q000378:metabolism*; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004852:Epoxy Compounds / Q000378:metabolism*; D005978:Glutathione / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D014325:Tromethamine / Q000494:pharmacology
D004852:Epoxy Compounds; D014325:Tromethamine; D016604:Aflatoxin B1; D005978:Glutathione
Etude, in vitro, de la conversion métabolique de l'aflatoxine B1 en son époxyde.