Isolation, and virulence profiles, of Aeromonas hydrophila implicated in an outbreak of food poisoning in Sweden.
A case of food poisoning outbreak involving Aeromonas hydrophila is reported in this study. A group of 27 people consumed a typical Swedish food "landgång" which is a type of smörgåsbord containing shrimps with mayonnaise, liver paté, ham, sausage, and legume salad which was purchased from a food store. Twenty-two of the 27 persons became ill within 20-34 hr of consumption of the food and reported the symptoms ranging from severe acute diarrhea, abdominal pain, headache, fever and vomiting. One person also fainted. The symptoms lasted for a couple of days. Of the remaining 5 healthy persons who consumed the left-over food the next day, 2 became ill with similar symptoms. The bacteriological examination of left-over food samples resulted in the isolation of A. hydrophila from shrimps with mayonnaise, smoked sausage, liver paté and boiled ham. The total number of A. hydrophila in these foods were log 10(6) to log > 10(7) organisms per gram of food sample. A. hydrophila was however, not isolated from legume/mayonnaise salad samples. All the food samples tested showed low numbers of other expected food contaminating organisms such as coliforms at 37 C and 44 C, fecal streptococci, Staphylococcus aureus, fungi and yeast etc., while Bacillus cereus, Clostridium perfringens and Salmonella spp. were not detected in the food samples. Investigations of the virulence profiles of the A. hydrophila isolates showed their capacity to produce beta-hemolysin, cytotoxins, cytotonic toxins, enterotoxins, and adhesion to and invasion of human intestinal (Henle 407) cells in culture.
Krovacek
K
K
Department of Veterinary Microbiology, Swedish University of Agricultural Sciences, Biomedical Center, Uppsala, Sweden.
Dumontet
S
S
Eriksson
E
E
Baloda
S B
SB
D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D016980:Aeromonas hydrophila / Q000302:isolation & purification* / Q000472:pathogenicity* / Q000502:physiology; D000368:Aged; D000818:Animals; D016466:CHO Cells / Q000382:microbiology; D002460:Cell Line; D002478:Cells, Cultured; D002648:Child; D006224:Cricetinae; D004196:Disease Outbreaks; D004768:Enterotoxins / Q000096:biosynthesis; D005516:Food Microbiology; D005517:Foodborne Diseases / Q000453:epidemiology* / Q000382:microbiology; D016905:Gram-Negative Bacterial Infections / Q000453:epidemiology* / Q000382:microbiology; D006801:Humans; D007223:Infant; D007422:Intestines / Q000382:microbiology; D008461:Meat Products / Q000382:microbiology / Q000506:poisoning; D008875:Middle Aged; D013548:Sweden / Q000453:epidemiology; D014774:Virulence
D004768:Enterotoxins