Citrinin affects the oxidative metabolism of BHK-21 cells.
The effects of citrinin on energy production along the respiratory chain and on glycolytic lactate production were examined in BHK-21 cultured cells. Citrinin inhibited the oxygen consumption rate by about 45 per cent. The respiratory rate of digitonin-treated cells energized with succinate, in the presence of ADP, was reduced by about 39 per cent. The mycotoxin inhibited the glucose utilization of BHK-21 cells by about 86 per cent. Cells treated with citrinin produced a small quantity of pyruvate, but were unable to produce lactate. It is concluded that BHK-21 cells cannot generate lactate when oxidative metabolism is inhibited by citrinin. The perturbations in BHK-21 cells caused by citrinin are due to alterations in mitochondrial function and in the glycolytic anaerobic pathway.
Chagas
G M
GM
Departamento de Bioquímica, Universidade Federal do Paraná, Curitiba, Brasil.
Campello
A P
AP
Kluppel
M L
ML
Oliveira
B M
BM
D000818:Animals; D002460:Cell Line / Q000201:enzymology; D019069:Cell Respiration / Q000502:physiology; D002953:Citrinin / Q000494:pharmacology*; D006224:Cricetinae; D004734:Energy Metabolism / Q000502:physiology*; D005947:Glucose / Q000378:metabolism; D006019:Glycolysis / Q000502:physiology; D007668:Kidney / Q000166:cytology; D008928:Mitochondria / Q000201:enzymology; D010085:Oxidative Phosphorylation
D002953:Citrinin; D005947:Glucose