The effect of p-chloroaniline on leucocytes of sheep peripheral blood under the migration-inhibition test conditions.
Toxic and immunotoxic effects of p-chloroaniline--a metabolite of herbicide monolinuron--were investigated in peripheral blood leucocytic suspensions of five sheep using a migration-inhibition test. The toxic effect of p-chloroaniline was recorded at concentrations 1.0 to 0.1 mg.ml-1 and the immunotoxic one at concentrations 0.01-0.001 mg.l-1. The toxic effect was demonstrated by total inhibition of leucocyte migration. The immunotoxic effect, determined as mitogenic activation of leucocytes by phytohemagglutinin, concanavalin A and lipopolysaccharide, was detected at 10 to 100-fold lower concentrations of p-chloroaniline than those which resulted in toxic effects.
Kacmár
P
P
University of Veterinary Medicine, Slovak Republic.
Pistl
J
J
Mikula
I
I
D000814:Aniline Compounds / Q000008:administration & dosage / Q000633:toxicity*; D000818:Animals; D001673:Biodegradation, Environmental; D002464:Cell Migration Inhibition; D005260:Female; D006540:Herbicides / Q000493:pharmacokinetics / Q000633:toxicity; D066298:In Vitro Techniques; D007962:Leukocytes / Q000187:drug effects* / Q000276:immunology / Q000502:physiology; D008044:Linuron / Q000031:analogs & derivatives / Q000493:pharmacokinetics / Q000633:toxicity; D008213:Lymphocyte Activation / Q000187:drug effects; D008297:Male; D008934:Mitogens / Q000494:pharmacology; D012756:Sheep
D000814:Aniline Compounds; D006540:Herbicides; D008934:Mitogens; D008044:Linuron; C030651:monolinuron; C004658:4-chloroaniline