title: Cloning of the mouse gene encoding plasma glutathione peroxidase: organization, sequence and chromosomal localization.
abstract: Using a reverse transcription coupled to PCR amplification strategy, with degenerated primers localized in highly conserved domains of known glutathione peroxidase (GPX) proteins, we have generated, from mouse epididymal RNA, a cDNA fragment which was subsequently used to isolate a genomic clone encoding mouse plasma GPX (GPX3). GPX3 is a major enzyme in reducing lipid hydroperoxides and hydrogen peroxide in plasma. We confirm here that the mouse epididymis is a new site of expression of GPX3 and report, together with the sequence, the structural analysis and the chromosomal localization of the mouse GPX3 single-copy gene to chromosome 11.
authors.affiliation: Laboratoire de Biologie Cellulaire, CNRS, URA1940, Université Blaise Pascal, Aubière, France.
authors.forename: V
authors.initials: V
authors.lastname: Schwaab
authors.forename: E
authors.initials: E
authors.lastname: Baud
authors.forename: N
authors.initials: N
authors.lastname: Ghyselinck
authors.forename: M G
authors.initials: MG
authors.lastname: Mattei
authors.forename: J P
authors.initials: JP
authors.lastname: Dufaure
authors.forename: J R
authors.initials: JR
authors.lastname: Drevet
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D016384:Consensus Sequence; D004822:Epididymis / Q000201:enzymology; D015870:Gene Expression; D005796:Genes; D005979:Glutathione Peroxidase / Q000097:blood / Q000235:genetics*; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics; D012045:Regulatory Sequences, Nucleic Acid; D015183:Restriction Mapping
chemical_list: D012333:RNA, Messenger; D005979:Glutathione Peroxidase