Modulation of carcinogen-induced DNA damage and repair enzyme activity by dietary riboflavin.
Formation of single strand breaks in nuclear DNA induced by hepatocarcinogens aflatoxin B1 and N-nitrosodimethylamine was observed to be more pronounced in rats maintained on a riboflavin-deficient diet compared to that on a normal diet. This increased damage was reversed on riboflavin supplementation. The induction of repair enzymes poly(ADP-ribose) polymerase, DNA polymerase beta and DNA ligase was significantly higher in riboflavin-deficient rats following DNA damage caused by the administration of carcinogens. Riboflavin supplementation brought down the induction to the levels found in rats maintained on normal diet. Since damage to DNA and its altered repair may relate to carcinogenesis, modulation of these parameters by riboflavin suggests a potential chemopreventive role of this vitamin.
Webster
R P
RP
Radiation Biology and Biochemistry Division, Bhabha Atomic Research Centre, Bombay, India.
Gawde
M D
MD
Bhattacharya
R K
RK
D016604:Aflatoxin B1 / Q000494:pharmacology; D000818:Animals; D002273:Carcinogens / Q000633:toxicity*; D004249:DNA Damage; D011088:DNA Ligases / Q000096:biosynthesis / Q000187:drug effects / Q000378:metabolism*; D004277:DNA, Single-Stranded / Q000187:drug effects / Q000378:metabolism; D004790:Enzyme Induction / Q000187:drug effects; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012256:Riboflavin / Q000494:pharmacology*; D012257:Riboflavin Deficiency / Q000201:enzymology
D002273:Carcinogens; D004277:DNA, Single-Stranded; D016604:Aflatoxin B1; D011088:DNA Ligases; D012256:Riboflavin