Phenotypic, molecular, and functional characterization of human peripheral blood CD34+/THY1+ cells.
A subset of mobilized CD34+ cells present in patient aphereses expresses Thy1 (CDw90). This population contains most long-term culture initiating cells, as assayed with a murine stromal cell line. It also contains a significant proportion of colony-forming unit granulocyte macrophage, but very few burst-forming unit erythroid. The limited differentiation towards the erythroid lineage is further confirmed by the absence of GATA-1 mRNA in the CD34+/Thy1+ subset, and by the low level of c-kit expression. The CD34+/Thy1+ subset appears phenotypically and functionally heterogeneous, a finding consistent with its high representation, compared to phenotypes such as CD34+/CD38-. Therefore, while at least some of CD34+/Thy1+ cells may be infectable by retroviral vectors, as shown by the presence of a transcript for the receptor for murine amphotropic retroviruses, the use of this selection strategy to specifically target human stem cells appears questionable.
Humeau
L
L
Département de Transfert de Gène et de Thérapie Génique: Institut Paoli Calmettes, Marseille, France.
Bardin
F
F
Maroc
C
C
Alario
T
T
Galindo
R
R
Mannoni
P
P
Chabannon
C
C
D018952:Antigens, CD34 / Q000032:analysis*; D001483:Base Sequence; D015415:Biomarkers; D001781:Blood Component Removal; D002454:Cell Differentiation; D002455:Cell Division; D002478:Cells, Cultured; D015672:Erythroid Precursor Cells / Q000166:cytology; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology; D006410:Hematopoiesis; D006412:Hematopoietic Stem Cells / Q000145:classification / Q000166:cytology*; D006801:Humans; D008969:Molecular Sequence Data; D010641:Phenotype; D011994:Recombinant Proteins / Q000494:pharmacology; D018800:Thy-1 Antigens / Q000032:analysis*
D018952:Antigens, CD34; D015415:Biomarkers; D011994:Recombinant Proteins; D018800:Thy-1 Antigens; D016179:Granulocyte Colony-Stimulating Factor