Construction of a combined NotI/SmaI physical and genetic map of Moraxella (Branhamella) catarrhalis strain ATCC25238.
Using pulsed field gel electrophoresis (PFGE) and Southern hybridization techniques, a physical map of Moraxella catarrhalis strain ATCC25238 was constructed to provide basic genetic knowledge of this bacterium that has attracted attention in recent years as a human pathogen. Restriction endonuclease NotI cut the genome into 10 fragments and SmaI into 9, and the molecular size of the genome was estimated to be 1,940 kilobases. Location of the 12 genes participating in the biosynthesis of purine, pyrimidine and nine kinds of amino acids were determined on the circular physical map of the strain.
Furihata
K
K
Department of Bacteriology, Shinshu University School of Medicine, Nagano, Japan.
Sato
K
K
Matsumoto
H
H
D000595:Amino Acid Sequence; D001482:Base Composition; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000302:isolation & purification; D015252:Deoxyribonucleases, Type II Site-Specific; D015723:Gene Library; D005798:Genes, Bacterial; D016680:Genome, Bacterial; D006801:Humans; D008969:Molecular Sequence Data; D008970:Molecular Weight; D001936:Moraxella catarrhalis / Q000235:genetics*; D015183:Restriction Mapping
D004269:DNA, Bacterial; C025651:CCCGGG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific; C038565:GCGGCCGC-specific type II deoxyribonucleases