Application of biotin, digoxigenin or fluorescein conjugated deoxynucleotides to label DNA strand breaks for analysis of cell proliferation and apoptosis using flow cytometry.
A flow cytometric method has recently been developed using biotinylated dUTP (b-dUTP) in a reaction catalyzed by terminal deoxynucleotidyl transferase (TdT) to identify the endonuclease-induced DNA strand breaks occurring during apoptosis. Counterstaining of DNA makes it possible to relate apoptosis to cell cycle position or DNA index. In the present study, we compared this method with one using digoxigenin-conjugated dUTP (d-dUTP) to label apoptotic cells. The discrimination of apoptotic from nonapoptotic cells was similar when incorporation of d-dUTP was compared with b-dUTP. Both techniques resulted in a 20-30 fold increase in staining of apoptotic over nonapoptotic cells although somewhat less background fluorescence was observed with the d-dUTP. Direct labeling with fluoresceinated dUTP (f-dUTP) was less sensitive in detecting DNA strand breaks, but had the advantage of simplicity. The principle of labeling DNA strand breaks using TdT was also employed to identify DNA replicating cells. To this end, the cells were incubated in the presence of BrdUrd, then exposed to UV light to selectively photolyse DNA containing the incorporated BrdUrd. DNA strand breaks resulting from the photolysis were then labeled with b-dUTP or d-dUTP. This approach is an alternative to immunocytochemical detection of BrdUrd incorporation, but unlike the latter does not require prior DNA denaturation, thus can be applied when the denaturation step must be avoided. The method was sensitive enough to recognize DNA synthesizing cells that were incubated with BrdUrd for only 5 min, the equivalent of replication of less than 1% of the cell's genome. The discrimination between apoptotic vs. BrdUrd incorporating-cells is based on different extractability of DNA following cell fixation. This method can be applied to analyze both cell proliferation (DNA replication) and death (by apoptosis) in a single measurement.
Li
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Cancer Research Institute, New York Medical College, Valhalla, New York 10595, USA.
James
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WM
Traganos
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Darzynkiewicz
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D017209:Apoptosis / Q000502:physiology*; D001710:Biotin / Q000031:analogs & derivatives* / Q000378:metabolism; D001853:Bone Marrow / Q000473:pathology; D001973:Bromodeoxyuridine / Q000378:metabolism; D002166:Camptothecin / Q000494:pharmacology; D002455:Cell Division / Q000502:physiology; D004247:DNA / Q000187:drug effects / Q000378:metabolism*; D004253:DNA Nucleotidylexotransferase / Q000378:metabolism; D015342:DNA Probes / Q000378:metabolism; D003856:Deoxyuracil Nucleotides / Q000378:metabolism*; D004076:Digoxigenin / Q000031:analogs & derivatives* / Q000378:metabolism; D005434:Flow Cytometry / Q000379:methods; D005452:Fluoresceins / Q000378:metabolism*; D018922:HL-60 Cells / Q000737:chemistry / Q000187:drug effects / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D007202:Indicators and Reagents / Q000378:metabolism; D011933:Reagent Kits, Diagnostic
D015342:DNA Probes; D003856:Deoxyuracil Nucleotides; D005452:Fluoresceins; D007202:Indicators and Reagents; D011933:Reagent Kits, Diagnostic; C070202:digoxigenin-11-deoxyuridine triphosphate; C069632:fluorescein-11-deoxyuridine triphosphate; D001710:Biotin; C045931:biotin-11-dUTP; D004247:DNA; D004253:DNA Nucleotidylexotransferase; D001973:Bromodeoxyuridine; D004076:Digoxigenin; D002166:Camptothecin