title: Crystallization and preliminary X-ray studies of isocitrate dehydrogenase from Thermus thermophilus HB8.
abstract: Isocitrate dehydrogenase from the thermophilic bacterium, Thermus thermophilus HB8, was crystallized by the vapor diffusion hanging drop method with polyethylene glycol 6000 as the precipitant. Pillar-like crystals of about 0.6 x 0.6 x 0.3 mm3 were obtained. Analysis of a series of oscillation photographs indicated that the orthorhombic crystals belonged to the I222 or I2(1)2(1)2(1) space group with unit cell dimensions of a = 100.1 A, b = 150.4 A, and c = 87.4 A. Intensity data were collected up to 2.5 A resolution.
authors.affiliation: Department of Life Science, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama.
authors.forename: M
authors.initials: M
authors.lastname: Ohzeki
authors.forename: T
authors.initials: T
authors.lastname: Yaoi
authors.forename: H
authors.initials: H
authors.lastname: Moriyama
authors.forename: T
authors.initials: T
authors.lastname: Oshima
authors.forename: N
authors.initials: N
authors.lastname: Tanaka
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D007521:Isocitrate Dehydrogenase / Q000737:chemistry*; D016963:Thermus thermophilus / Q000201:enzymology*
chemical_list: D007521:Isocitrate Dehydrogenase