Salmonella typhi does not inhibit phagosome-lysosome fusion in human monocyte-derived macrophages.
We examined phagosome-lysosome fusion in Salmonella typhi-infected human monocyte-derived macrophages and its relevance to the intracellular survival of this bacterium in vitro. S. typhi was found to survive and multiply in human monocyte-derived macrophages, whereas S. typhimurium was killed easily, indicating that the survival of Salmonella serovars is host-specific. Neither S. typhi nor S. typhimurium inhibited phagosome-lysosome fusion in human monocyte-derived macrophages. No difference between the phagosome-lysosome fusibilities of freshly prepared human monocytes and monocyte-derived macrophages was observed. These results suggest that S. typhi may survive by adapting to the conditions within fused phagolysosomes of human monocyte-derived macrophages.
Ishibashi
Y
Y
Department of Microbiology, Meiji College of Pharmacy, Tokyo, Japan.
Arai
T
T
D003602:Cytotoxicity, Immunologic; D006801:Humans; D008247:Lysosomes / Q000276:immunology*; D008264:Macrophages / Q000276:immunology / Q000382:microbiology*; D009000:Monocytes / Q000276:immunology / Q000382:microbiology; D010587:Phagocytosis / Q000276:immunology; D010588:Phagosomes / Q000276:immunology*; D012485:Salmonella typhi / Q000254:growth & development / Q000276:immunology*; D012486:Salmonella typhimurium / Q000378:metabolism; D013997:Time Factors