The zeta protein kinase C isoform from the testis of the grey mullet Mugil cephalus with a specific reaction protein of M(r) 48,000 on oolemma.
The zeta protein kinase C isoform (PKC-zeta) was purified from the testis of the grey mullet Mugil cephalus and has relative masses (M(r)) of 65,000 and 63,000. The subunits of PKC-zeta from spermatozoa degenerated to M(r) 58,000 and 53,000 after continuous freezing and thawing. Proteins of M(r) 48,000 on the oolemma of the grey mullet Mugil cephalus were found to be the reaction proteins of the PKC-zeta from spermatozoa.
Huang
C F
CF
Division of Biochemistry and Molecular Science, Institute of Zoology, Academia Sinica, Taipei, Taiwan, Republic of China.
Chen
H C
HC
Chuang
N N
NN
Kuo
C M
CM
D000595:Amino Acid Sequence; D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D007527:Isoenzymes / Q000302:isolation & purification / Q000378:metabolism*; D008297:Male; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009865:Oocytes / Q000166:cytology / Q000378:metabolism; D010473:Perciformes; D011493:Protein Kinase C / Q000302:isolation & purification / Q000378:metabolism*; D051744:Protein Kinase C-epsilon; D013094:Spermatozoa / Q000378:metabolism; D013737:Testis / Q000378:metabolism*; D015044:Zona Pellucida / Q000378:metabolism*
D007527:Isoenzymes; D011493:Protein Kinase C; D051744:Protein Kinase C-epsilon