Growth and cytopathic effect of Japanese encephalitis virus in astrocyte-enriched cell cultures from neonatal mouse brains.
Neuron-free, astrocyte-enriched brain cell cultures from newborn mice could be infected with Japanese encephalitis virus (JEV) as evidenced by immunofluorescence (IF), viral replication and cytopathic effect (CPE). Virus-specific cytoplasmic fluorescence was detectable in astrocytes first after 18 hrs, released infectious progeny virus in the culture fluid after 24 hrs, and CPE after 11 days post infection (p.i.).
Suri
N K
NK
National Institute of Virology, Pune, India.
Banerjee
K
K
D000818:Animals; D000831:Animals, Newborn; D001253:Astrocytes / Q000166:cytology*; D001921:Brain / Q000166:cytology*; D002478:Cells, Cultured; D003588:Cytopathogenic Effect, Viral; D004664:Encephalitis Virus, Japanese / Q000254:growth & development* / Q000502:physiology; D051379:Mice