Newly designed hemostatic technology based on photocurable gelatin.
In this article, the authors present a novel photochemically driven hemostatic technology using photocurable gelatins partially derivatized with photoreactive xanthene dyes (fluorescein, eosin, and rose bengal) and a hydrophilic difunctional macromer. The developed hemostatic glue consisted of dye derivatized gelatin (20 wt%), poly(ethylene glycol) diacrylate (10 wt%), and ascorbid acid (0.3 wt%), all of which were dissolved in a saline solution. Irradiation of the hemostatic glue by visible light produced a swollen gel within a few tenths of a second due to dye sensitized photo-crosslinking and photograft polymerization. An increase in irradiation time resulted in an increased gel yield and reduced water swellability. A rat liver injured on laparotomy was coated with the hemostatic glue. Upon visible light irradiation through an optical fiber, the coated viscous solution was immediately converted to a swollen gel and, concomitantly, hemostasis was completed. Histologic examination showed that, at 7 days after surgery, little gelatin remained in the injured region, scarring with little necrosis occurred, and inflammatory cells infiltrated from the surrounding tissue and tissue regeneration proceeded well. During laparoscopic surgery, in situ gelation of the hemostatic glue on the liver surface was demonstrated using a specially designed fiberscope.
Nakayama
Y
Y
Department of Bioengineering, National Cardiovascular Center Research Institute, Osaka, Japan.
Matsuda
T
T
D000818:Animals; D004396:Coloring Agents; D005069:Evaluation Studies as Topic; D005780:Gelatin / Q000528:radiation effects; D006489:Hemostatic Techniques; D020706:Laparoscopes; D008099:Liver / Q000293:injuries; D010777:Photochemistry; D051381:Rats; D017208:Rats, Wistar; D014014:Tissue Adhesives / Q000528:radiation effects
D004396:Coloring Agents; D014014:Tissue Adhesives; D005780:Gelatin