Interleukin-1 and interleukin-6 gene expression in human monocytes stimulated with Salmonella typhimurium porins.
The aim of this study was to verify whether Salmonella typhimurium porins can affect the expression of interleukin-1 (IL-1) and interleukin-6 (IL-6) genes. Human monocytes were treated with porins, and total RNAs were analysed by Northern blotting to evaluate the expression of IL-1 alpha, IL-1 beta and IL-6 in both treated and untreated cell cultures. Porins induced a significant increase in IL-1 and IL-6 transcripts. This increase was related to the dose of porins, and it peaked 5 hr after treatment. The same results were obtained when polymyxin B was added to the porin preparation to eliminate eventual traces of lipopolysaccharide (LPS) associated with porins. The porins-mediated increase in interleukin transcripts did not require de novo protein synthesis, and it was because of the enhanced half-life of IL-1 and IL-6 mRNAs, rather an increased rate of gene transcription. These data suggest that porins may affect inflammatory and immunological responses by enhancing the expression of cytokine genes.
Galdiero
M
M
Department of Pathology, Prophylaxis and Food Care, Frederick II Naples University, School of Veterinary Medicine, Italy.
Cipollaro de L'ero
G
G
Donnarumma
G
G
Marcatili
A
A
Galdiero
F
F
D015152:Blotting, Northern; D018929:Cell Culture Techniques; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D007375:Interleukin-1 / Q000235:genetics* / Q000378:metabolism; D015850:Interleukin-6 / Q000235:genetics* / Q000378:metabolism; D009000:Monocytes / Q000276:immunology*; D018272:Porins / Q000276:immunology* / Q000494:pharmacology; D014176:Protein Biosynthesis; D012313:RNA / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D012486:Salmonella typhimurium / Q000276:immunology*; D014158:Transcription, Genetic
D007375:Interleukin-1; D015850:Interleukin-6; D018272:Porins; D012333:RNA, Messenger; D012313:RNA