Cloning, sequencing, and characterization of a complementary DNA from Haematobia irritans irritans (Diptera: Muscidae) that shares significant homology to a yeast ATPase.
A study of the molecular genetics of the horn fly, Haematobia irritans irritans (L.), has resulted in the isolation and sequencing of a 2316-bp complementary DNA (cDNA) clone, HFN2b. An open reading frame was found, the deduced 464 amino acid sequence of which encodes a protein with 40% identity to AFG1, a member of a yeast family of ATPases; it contains an ATP- or GTP-binding P-loop motif. Northern and Southern blot analyses do not reveal significant differences in the expression of the gene homologous to HFN2b when comparing laboratory-reared horn flies that are susceptible or resistant to pyrethroid insecticides.
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Knipling-Bushland U.S. Livestock Insects Research Laboratory, USDA-ARS, Kerrville, TX 78028, USA.
D000251:Adenosine Triphosphatases / Q000737:chemistry / Q000235:genetics*; D000255:Adenosine Triphosphate / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D009033:Culicidae / Q000201:enzymology* / Q000254:growth & development; D017931:DNA Primers; D018076:DNA, Complementary; D006160:Guanosine Triphosphate / Q000378:metabolism; D008969:Molecular Sequence Data; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D017386:Sequence Homology, Amino Acid
D017931:DNA Primers; D018076:DNA, Complementary; D006160:Guanosine Triphosphate; D000255:Adenosine Triphosphate; D000251:Adenosine Triphosphatases