Locations of the ets subfamily members net, elk1, and sap1 (ELK3, ELK1, and ELK4) on three homologous regions of the mouse and human genomes.
Net, Elk1, and Sap1 are related members of the Ets oncoprotein family. We show by in situ hybridization on banded chromosomes with specific cDNA probes that their map positions on mouse and human chromosomes (respectively) are net, 10C-D1 and 12q22-q23 (now called ELK3), sap1, 1E3-G and 1q32 (ELK4), and elk1, XA1-A3 and Xp11.2-p11.1 (ELK1), as well as a second locus 14q32 (ELK2) unique to the human genome. The results for the mouse net, sap1, and elk1 and human ELK3 genes are new. The human elk1 mapping confirms a previous study. The human ELK4 localization agrees with data published during the preparation of the manuscript. Human ELK3 colocalizes with sap2, and we confirm that they are identical. These results firmly establish for the first time that Net, Elk1, and Sap1 are distinct gene products with different chromosomal localizations in both the mouse and the human genomes. Net, Elk1, and Sap1 are conserved and map to homologous regions of the mouse and human chromosomes.
Giovane
A
A
Institut de Génétique et de Biologie Moléculaire et Cellulaire, Illkirch, France.
Sobieszczuk
P
P
Mignon
C
C
Mattei
M G
MG
Wasylyk
B
B
D000818:Animals; D002874:Chromosome Mapping; D002878:Chromosomes, Human, Pair 1; D002883:Chromosomes, Human, Pair 14; D004268:DNA-Binding Proteins / Q000235:genetics*; D016678:Genome; D015894:Genome, Human; D006801:Humans; D051379:Mice; D015513:Oncogene Proteins; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D050783:Proto-Oncogene Proteins c-ets; D029702:Schizosaccharomyces pombe Proteins; D014157:Transcription Factors / Q000235:genetics*; D014960:X Chromosome; D050807:ets-Domain Protein Elk-1
D004268:DNA-Binding Proteins; C494112:ELK1 protein, human; C494113:Elk1 protein, mouse; C086924:Elk3 protein, mouse; D015513:Oncogene Proteins; D011518:Proto-Oncogene Proteins; D050783:Proto-Oncogene Proteins c-ets; C085981:Sap1 protein, S pombe; D029702:Schizosaccharomyces pombe Proteins; D014157:Transcription Factors; D050807:ets-Domain Protein Elk-1