Modulation of adenosine-induced cAMP accumulation via metabotropic glutamate receptors in chick optic tectum.
Changes on cyclic adenosine monophosphate (cAMP) levels in response to adenosine and glutamate and the subtype of glutamate receptors involved in this interaction were studied in slices of optic tectum from 3-day-old chicks. cAMP accumulation mediated by adenosine (100 microM) was abolished by 8-phenyltheophylline (15 microM). Glutamate and the glutamatergic agonists kainate or trans-D, L-1-aminocyclopentane-1,3-dicarboxylic acid (trans-ACPD) did not evoke cAMP accumulation. Glutamate blocked the adenosine response in a dose-dependent manner. At 100 microM, glutamate did not inhibit the effect of adenosine. The 1 mM and 10 mM doses of glutamate inhibited adenosine-induced cAMP accumulation by 55% and 100%, respectively. When glutamatergic antagonists were used, this inhibitory effect was not affected by 200 microM 6,7-dihydroxy-2,3,dinitroquinoxaline (DNQX), an ionotropic antagonist, and was partially antagonized by 1 mM (RS)-alpha-methyl-4-carboxyphenylglycine [(RS)M-CPG], a metabotropic antagonist, while 1 mM L-2-amino-3-phosphonopropionate (L-AP3) alone, another metabotropic antagonist, presented the same inhibitory effect of glutamate. Kainate (10 mM) and trans-ACPD (100 microM and 1 mM) partially blocked the adenosine response. This study indicates the involvement of metabotropic glutamate receptors in adenylate cyclase inhibition induced by glutamate and its agonists trans-ACPD and kainate.
Tasca
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Departamento de Bioquimica, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brasil.
Vendite
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Martini
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Cardoso
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Souza
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D000241:Adenosine / Q000494:pharmacology*; D000409:Alanine / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D001565:Benzoates; D002645:Chickens; D000242:Cyclic AMP / Q000378:metabolism*; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology; D018698:Glutamic Acid / Q000031:analogs & derivatives / Q000494:pharmacology*; D005998:Glycine / Q000031:analogs & derivatives; D066298:In Vitro Techniques; D008297:Male; D011810:Quinoxalines / Q000494:pharmacology; D018094:Receptors, Metabotropic Glutamate / Q000502:physiology*; D013477:Superior Colliculi / Q000187:drug effects*
D001565:Benzoates; D018691:Excitatory Amino Acid Antagonists; D011810:Quinoxalines; D018094:Receptors, Metabotropic Glutamate; C000211:2-amino-3-phosphonopropionic acid; C079694:alpha-methyl-4-carboxyphenylglycine; D018698:Glutamic Acid; C056723:FG 9041; D000242:Cyclic AMP; D000241:Adenosine; D000409:Alanine; D005998:Glycine