[Study of mitochondrial DNA in Helix land snails by PCR-RFLP].
Sometimes the morphological criteria--used to distinguish between 4 land snails species (Helix pomatia, Helix lucorum, Helix aspersa aspersa and Helix aspersa maxima)--are little pronounced and ambiguous. We have selected mitochondrial DNA, a widely used molecular marker, in order to have a reliable technique of distinction between the 4 studied species. After extraction, mtDNA is amplified by PCR and digested with several enzymes. Among them, Dra I give restriction fragments of different length depending on species. So, owing to PCR-RFLP of mtDNA, we describe here a reliable method for identifying 4 snail species.
Borgo
R
R
Laboratoire de Zoologie Embryologie, UFR Sciences et Techniques, Besançon.
Souty-Grosset
C
C
Gomot
L
L
D000818:Animals; D004272:DNA, Mitochondrial / Q000737:chemistry*; D006372:Helix, Snails / Q000145:classification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length
D004272:DNA, Mitochondrial
Etude de l'ADN mitochondrial d'espèces d'escargots du genre Helix par PCR-RFLP.