title: Mycobacterium paratuberculosis and inflammatory bowel disease: frequency distribution in serial colonoscopic biopsies using the polymerase chain reaction.
abstract: An association between Mycobacterium paratuberculosis and Crohn's disease is suspected but the evidence remains controversial. Using a one-step DNA extraction procedure with the thermophilic protease PRETAQ and amplification by the polymerase chain reaction, M. paratuberculosis DNA was detected in 22% of patients with Crohn's disease, and in 13% of patients with ulcerative colitis. M. paratuberculosis DNA was not found in any biopsy tissue from control non-inflammatory bowel disease patients. The biopsy tissues in which M. paratuberculosis was detected all came from regions which were inflamed when viewed microscopically. Overall, 7.7% of biopsies from such inflamed areas were positive. This low frequency of detection could be explained on the basis of extremely low abundance of the organism in relation to the area of mucosa sampled, or be consistent with a non-aetiological role for M. paratuberculosis in inflammatory bowel disease.
authors.affiliation: Department of Veterinary Pathology and Public Health, Massey University, Palmerston North, New Zealand.
authors.forename: A
authors.initials: A
authors.lastname: Murray
authors.forename: J
authors.initials: J
authors.lastname: Oliaro
authors.forename: M M
authors.initials: MM
authors.lastname: Schlup
authors.forename: V S
authors.initials: VS
authors.lastname: Chadwick
mesh_terms: D000818:Animals; D001483:Base Sequence; D001706:Biopsy; D003093:Colitis, Ulcerative / Q000382:microbiology / Q000473:pathology; D003113:Colonoscopy; D003424:Crohn Disease / Q000382:microbiology / Q000473:pathology; D004269:DNA, Bacterial / Q000032:analysis; D005260:Female; D006801:Humans; D015212:Inflammatory Bowel Diseases / Q000382:microbiology* / Q000473:pathology; D008969:Molecular Sequence Data; D009164:Mycobacterium Infections / Q000150:complications*; D016927:Mycobacterium avium subsp. paratuberculosis / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity
chemical_list: D004269:DNA, Bacterial