title: Transcription and localization of growth factor mRNA in the bovine oviduct.
abstract: It has become evident that certain growth factors are involved in the regulation of the initial bovine embryogenesis. In the present study, we examined by means of Northern blot and in situ hybridization, the expression and localization in the bovine oviduct of mRNAs encoding for platelet-derived growth factor (PDGF-B), basic fibroblast growth factor (bFGF), and insulin-like growth factor (IGF-I). Northern blot analysis on oviduct tissue demonstrated transcripts for PDGF-B and bFGF, but not IGF-I mRNAs. Two bands with estimated sizes of 5.0 and 1.5 kb were detected for PDGF-B and two bands with sizes of 7.5 and 4.9 kb for bFGF. In situ hybridization analysis demonstrated localization of PDGF-B mRNA in the lamina epithelialis and tunica muscularis of the oviduct whereas bFGF mRNA was detected in the lamina propria. It is concluded that the lamina epithelialis and lamina propria of the oviduct represent sites of synthesis of PDGF-B and bFGF mRNA, respectively.
authors.affiliation: Department of Anatomy and Physiology, Royal Veterinary and Agricultural University, Frederiksberg C, Denmark.
authors.forename: D
authors.initials: D
authors.lastname: Viuff
authors.forename: P
authors.initials: P
authors.lastname: Hyttel
authors.forename: T
authors.initials: T
authors.lastname: Greve
authors.forename: T
authors.initials: T
authors.lastname: Eriksen
authors.forename: S
authors.initials: S
authors.lastname: Alexandersen
mesh_terms: D000818:Animals; D002417:Cattle; D005314:Embryonic and Fetal Development; D004848:Epithelium / Q000378:metabolism; D005187:Fallopian Tubes / Q000378:metabolism* / Q000648:ultrastructure; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000096:biosynthesis / Q000235:genetics*; D007334:Insulin-Like Growth Factor I / Q000096:biosynthesis / Q000235:genetics*; D010062:Ovulation Induction; D010982:Platelet-Derived Growth Factor / Q000096:biosynthesis / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D010982:Platelet-Derived Growth Factor; D012333:RNA, Messenger; D016222:Fibroblast Growth Factor 2; D007334:Insulin-Like Growth Factor I