Inhibitory effect of 3'-azido-3'-deoxythymidine on proliferative responsiveness of CD8+ lymphocytes to interleukin-2.
Although several studies have shown that 3'-azido-3'-deoxythymidine (AZT) is not toxic for CD4+ lymphocytes, its effect on CD8+ cells has never been studied in a systematic way. We purified CD8+ cells from the peripheral blood mononuclear cells of both human immunodeficiency virus (HIV)-seronegative and HIV-infected individuals by means of magnetic beads that had been coated with monoclonal antibodies. We report that AZT, but not two other nucleosides tested, inhibited the interleukin-2-dependent proliferation of CD8+ lymphocytes in a dose-dependent manner. No such effect was observed with regard to CD4(+)-enriched populations. The AZT-mediated antiproliferative effect did not appear to be related to either the CD4+ count or to prior treatment with this drug in the case of HIV-seropositive subjects.
Mercure
L
L
McGill University AIDS Centre, Montreal, Quebec, Canada.
Lalonde
R
R
Phaneuf
D
D
Brenner
B
B
Wainberg
M A
MA
D000998:Antiviral Agents / Q000276:immunology* / Q000494:pharmacology; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D002455:Cell Division / Q000276:immunology; D016049:Didanosine / Q000276:immunology / Q000494:pharmacology; D006801:Humans; D007376:Interleukin-2 / Q000037:antagonists & inhibitors* / Q000276:immunology; D019259:Lamivudine; D008213:Lymphocyte Activation / Q000187:drug effects; D016047:Zalcitabine / Q000031:analogs & derivatives / Q000276:immunology / Q000494:pharmacology; D015215:Zidovudine / Q000276:immunology / Q000494:pharmacology*
D000998:Antiviral Agents; D007376:Interleukin-2; D019259:Lamivudine; D015215:Zidovudine; D016047:Zalcitabine; D016049:Didanosine