2,4-Dichlorophenoxyacetic acid influx is mediated by an active transport system in Chinese hamster ovary cells.
The influx of 2,4-dichlorophenoxyacetic acid (2,4-D) into Chinese hamster ovary (CHO) cells was studied. The cells mainly took up but did not metabolize the undissociated form of the herbicide. The uptake of 2,4-D was carried out against a concentration gradient and was inhibited by sodium azide and dinitrophenol. The results presented here show that the herbicide influx was an active, energy dependent process. (Na+ + K+)ATPase does not seem to be involved because ouabain, an inhibitor of the enzyme, did not affect the 2,4-D uptake.
Bergesse
J R
JR
Departamento de Biología Molecular, Universidad Nacional de Rio Cuarto, Córdoba, Argentina.
Balegno
H F
HF
D015084:2,4-Dichlorophenoxyacetic Acid / Q000493:pharmacokinetics* / Q000633:toxicity; D019325:3-O-Methylglucose; D000621:Aminoisobutyric Acids / Q000493:pharmacokinetics; D000818:Animals; D001386:Azides / Q000494:pharmacology; D001693:Biological Transport, Active / Q000502:physiology; D016466:CHO Cells; D006224:Cricetinae; D017077:Culture Media, Conditioned; D004140:Dinitrophenols / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006540:Herbicides / Q000493:pharmacokinetics* / Q000633:toxicity; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008757:Methylglucosides / Q000493:pharmacokinetics; D010042:Ouabain / Q000494:pharmacology; D016196:S Phase / Q000187:drug effects; D019810:Sodium Azide; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors
D000621:Aminoisobutyric Acids; D001386:Azides; D017077:Culture Media, Conditioned; D004140:Dinitrophenols; D004791:Enzyme Inhibitors; D006540:Herbicides; D008757:Methylglucosides; D019325:3-O-Methylglucose; C100049:2-aminoisobutyric acid; D015084:2,4-Dichlorophenoxyacetic Acid; D010042:Ouabain; D019810:Sodium Azide; D000254:Sodium-Potassium-Exchanging ATPase