Crystallization and preliminary X-ray analysis of fructose 6-phosphate, 2-kinase:fructose 2,6-bisphosphatase.
Diffraction-quality crystals of the bifunctional enzyme fructose 6-phosphate, 2-kinase:fructose 2,6-bisphosphatase from rat testis have been obtained. The crystals were grown in the presence of ATP gamma S, fructose 6-phosphate, the detergent n-octylglucoside, and the precipitant polyethylene glycol 4000. The crystals have the symmetry of the trigonal space group P31/221 with a = b = 83.0 A and c = 130.6 A. Flash-frozen crystals diffract to beyond 2.2 A, and native data have been collected.
Istvan
E S
ES
Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas 75235-9050, USA.
Hasemann
C A
CA
Kurumbail
R G
RG
Uyeda
K
K
Deisenhofer
J
J
D000255:Adenosine Triphosphate / Q000031:analogs & derivatives; D000818:Animals; D003460:Crystallization; D018360:Crystallography, X-Ray; D005636:Fructosephosphates; D005960:Glucosides; D008297:Male; D009097:Multienzyme Complexes / Q000737:chemistry*; D025481:Phosphofructokinase-2; D010744:Phosphoric Monoester Hydrolases / Q000737:chemistry*; D010770:Phosphotransferases / Q000737:chemistry*; D051381:Rats; D013737:Testis / Q000201:enzymology
D005636:Fructosephosphates; D005960:Glucosides; D009097:Multienzyme Complexes; C018619:octyl-beta-D-glucoside; C022571:adenosine 5'-O-(3-thiotriphosphate); C027618:fructose-6-phosphate; D000255:Adenosine Triphosphate; D010770:Phosphotransferases; D025481:Phosphofructokinase-2; D010744:Phosphoric Monoester Hydrolases