title: Identification of mRNAs that show modulated expression during colon carcinoma cell differentiation.
abstract: To investigate the working hypotheses that stem cells or their early descendants are prime targets for neoplastic transformation, and that the degree to which a neoplasm retains the immature phenotype is an important determinant of tumor aggressiveness, we have identified several mRNAs that are downregulated during the in vitro differentiation of HT29-D4 colon carcinoma cells. These genes include heat-shock cognate protein Hsc70, adenylosuccinate lyase, B23/nucleophosmin, alpha-tubulin, and a novel gene designated DS-1. The DS-1 mRNA has a length of approximately 0.9 kb and is downregulated 4.7-fold upon differentiation. From the DS-1 cDNA, a protein of 206 amino acids with a molecular mass of 24 kDa and an isoelectric point of 10.9 can be deduced. An antiserum directed against a synthetic peptide detected a minor band of the expected size in Western blots, as well as a major band of lower size that may represent a processed form of the protein.
authors.affiliation: Department of Pathology, Erasmus University, Rotterdam, The Netherlands.
authors.forename: N
authors.initials: N
authors.lastname: van Belzen
authors.forename: M P
authors.initials: MP
authors.lastname: Diesveld
authors.forename: A C
authors.initials: AC
authors.lastname: van der Made
authors.forename: Y
authors.initials: Y
authors.lastname: Nozawa
authors.forename: W N
authors.initials: WN
authors.lastname: Dinjens
authors.forename: R
authors.initials: R
authors.lastname: Vlietstra
authors.forename: J
authors.initials: J
authors.lastname: Trapman
authors.forename: F T
authors.initials: FT
authors.lastname: Bosman
mesh_terms: D000264:Adenylosuccinate Lyase / Q000096:biosynthesis / Q000235:genetics; D000595:Amino Acid Sequence; D001483:Base Sequence; D015153:Blotting, Western; D002454:Cell Differentiation / Q000235:genetics*; D003110:Colonic Neoplasms / Q000235:genetics* / Q000473:pathology; D017931:DNA Primers; D018076:DNA, Complementary; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis / Q000235:genetics; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000096:biosynthesis / Q000235:genetics; D000090243:Nucleophosmin; D010750:Phosphoproteins / Q000096:biosynthesis / Q000235:genetics; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D012269:Ribosomal Proteins; D014404:Tubulin / Q000096:biosynthesis / Q000235:genetics; D014407:Tumor Cells, Cultured
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D018840:HSP70 Heat-Shock Proteins; C098021:MRPL58 protein, human; C000716967:NPM1 protein, human; D009687:Nuclear Proteins; D010750:Phosphoproteins; D011506:Proteins; D012333:RNA, Messenger; D012269:Ribosomal Proteins; D014404:Tubulin; D000090243:Nucleophosmin; D000264:Adenylosuccinate Lyase