Cloning and expression of a single-chain antibody fragment specific for a monomorphic determinant of class I molecules of the human major histocompatibility complex.
B9.12.1 is a monoclonal antibody specific for a monomorphic determinant of human MHC class I molecules. It is currently used for cell typing and is useful for targeting infection of human cells by murine ecotropic retroviruses. We have cloned and expressed it in the form of a single-chain variable fragment (ScFv) that recognizes the same epitope as the parental antibody. Through genetic engineering, this ScFv may be used for developing new cell-typing probes and new retroviral targeting approaches.
Marin
M
M
Institut de Génétique Moléculaire de Montpellier, UMR9942/CNRS, France.
Brockly
F
F
Noel
D
D
Etienne-Julan
M
M
Biard-Piechaczyk
M
M
Hua
T D
TD
Gu
Z J
ZJ
Piechaczyk
M
M
D000595:Amino Acid Sequence; D000918:Antibody Specificity; D001483:Base Sequence; D001666:Binding Sites, Antibody; D003001:Cloning, Molecular; D000939:Epitopes / Q000737:chemistry / Q000276:immunology*; D015870:Gene Expression / Q000276:immunology; D005803:Genes, Immunoglobulin; D005805:Genes, MHC Class I / Q000276:immunology; D015395:Histocompatibility Antigens Class I / Q000235:genetics* / Q000276:immunology*; D006801:Humans; D007135:Immunoglobulin Variable Region / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data
D000939:Epitopes; D015395:Histocompatibility Antigens Class I; D007135:Immunoglobulin Variable Region