title: Hepatitis B virus (HBV)-specific in vitro antibody production by peripheral blood mononuclear cells (PBMC) after vaccination by recombinant hepatitis B surface antigen (rHBsAg).
abstract: To study the immunization induced by rHBsAg, we analysed the in vitro antibody production (IVAP) to HBsAg by PBMC from 18 subjects vaccinated by two injections on days 0 and 30. HBsAg-specific IVAP was detectable in all subjects after both the first and the second injection, and lasted for about 10 days and then disappeared. However, when the spontaneous HBsAg-specific IVAP became negative, HBsAg stimulation of PBMC cultures induced again a specific HBsAg IVAP. Cultures of cell populations separated by erythrocyte rosetting or Percoll density centrifugation showed that the cells responsible for spontaneous secretion, after in vivo stimulation, were low-density B lymphocytes. High-density B lymphocytes were involved in anti-HBs production induced by in vitro stimulation when spontaneous secretion disappeared. These data suggest that the IVAP test could be a source of important information along with serologic analysis for exploration of the immune response to hepatitis B vaccine.
authors.affiliation: Département d'Immunologie, Hôpital Lapeyronie, Montpellier, France.
authors.forename: J
authors.initials: J
authors.lastname: Ducos
authors.forename: A M
authors.initials: AM
authors.lastname: Bianchi-Mondain
authors.forename: G
authors.initials: G
authors.lastname: Pageaux
authors.forename: A M
authors.initials: AM
authors.lastname: Conge
authors.forename: R
authors.initials: R
authors.lastname: Poncet
authors.forename: J P
authors.initials: JP
authors.lastname: Vendrell
authors.forename: M
authors.initials: M
authors.lastname: Segondy
authors.forename: A
authors.initials: A
authors.lastname: Serre
mesh_terms: D000328:Adult; D002478:Cells, Cultured; D005260:Female; D006510:Hepatitis B Antibodies / Q000096:biosynthesis* / Q000097:blood; D006514:Hepatitis B Surface Antigens / Q000276:immunology*; D017325:Hepatitis B Vaccines / Q000276:immunology*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008213:Lymphocyte Activation; D008297:Male; D011994:Recombinant Proteins / Q000276:immunology; D013997:Time Factors; D014614:Vaccines, Synthetic / Q000276:immunology*
chemical_list: D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens; D017325:Hepatitis B Vaccines; D011994:Recombinant Proteins; D014614:Vaccines, Synthetic