Gene transfer by jet injection into differentiated tissues of living animals and in organ culture.
Jet injection can be used to introduce genes into the cells of differentiated tissues of living animals and organ cultures. When a solution of plasmid DNA is jet injected into a selected tissue or organ, cells lying in or near the path of the jet injection are transfected with the DNA and the introduced gene(s) are expressed. Since there is minimal morbidity from each jet injection, multiple injections can be performed at the same or nearby sites. Both mRNA and protein expression from transfected genes can be quantitated using standard methods. In addition, the technique is an efficient means of DNA immunization. Methodology for using jet injection to transfer plasmid DNA into the cells of skin, fat, mammary gland, and muscle are described.
Furth
P A
PA
Department of Medicine, University of Maryland School of Medicine, Baltimore, USA.
Kerr
D
D
Wall
R
R
D000273:Adipose Tissue; D000818:Animals; D001709:Biotechnology; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D005069:Evaluation Studies as Topic; D005260:Female; D015870:Gene Expression; D018014:Gene Transfer Techniques / Q000295:instrumentation; D017930:Genes, Reporter; D007277:Injections, Jet; D008156:Luciferases / Q000235:genetics; D008321:Mammary Glands, Animal; D051379:Mice; D009132:Muscles; D009924:Organ Culture Techniques; D010957:Plasmids / Q000008:administration & dosage / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011817:Rabbits; D012867:Skin; D001616:beta-Galactosidase / Q000235:genetics
D012333:RNA, Messenger; D008156:Luciferases; D015500:Chloramphenicol O-Acetyltransferase; D001616:beta-Galactosidase