The controversial role of cAMP on amnionic prostaglandin release: effect of adenylate cyclase inhibition.
The suggested role of cAMP in the regulation of amnionic prostaglandin release was investigated using two adenylate cyclase inhibitors, MDL 12330A and SQ 22536. These substances exhibited a dose-dependent inhibitory effect on both amnionic enzyme and cAMP levels, but they did not influence prostaglandin E (PGE) release. In addition forskolin and IBMX (3-isobutyl-1-methylxanthine), two drugs known to increase cAMP levels, did not affect PGE output, while dibutyryl cyclic cAMP showed a dose-dependent inhibitory effect. On the basis of our data, the suggested role of amnionic adenylate cyclase in triggering prostaglandin release is not confirmed, and the pathway of phospholipase A2 activation at the onset of labor remains to be elucidated.
Vesce
F
F
Istituto di Clinica Ostetrica e Ginecologica, Università degli Studi di Ferrara, Italy.
Biondi
C
C
Ferretti
M E
ME
Pareschi
M C
MC
Buzzi
M
M
Colamussi
M L
ML
D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D000225:Adenine / Q000031:analogs & derivatives* / Q000494:pharmacology; D000067956:Adenylyl Cyclase Inhibitors; D000262:Adenylyl Cyclases / Q000032:analysis; D000650:Amnion / Q000187:drug effects* / Q000378:metabolism; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000037:antagonists & inhibitors / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation; D005260:Female; D006801:Humans; D007097:Imines / Q000494:pharmacology*; D010741:Phospholipases A / Q000378:metabolism / Q000502:physiology; D054467:Phospholipases A2; D011247:Pregnancy; D011453:Prostaglandins / Q000378:metabolism*; D011458:Prostaglandins E / Q000378:metabolism
D000067956:Adenylyl Cyclase Inhibitors; D007097:Imines; D011453:Prostaglandins; D011458:Prostaglandins E; C017759:9-(tetrahydro-2-furyl)-adenine; D005576:Colforsin; C014925:RMI 12330A; D000242:Cyclic AMP; D010741:Phospholipases A; D054467:Phospholipases A2; D000262:Adenylyl Cyclases; D000225:Adenine; D015056:1-Methyl-3-isobutylxanthine