title: Heat shock proteins of Toxoplasma gondii.
abstract: We have investigated heat shock protein (HSP) expression in mouse-virulent and -avirulent strains of Toxoplasma gondii by performing Western blot analysis using a monoclonal antibody against HSP65 of Mycobacterium bovis and a polyclonal antiserum against HSP70 of Plasmodium falciparum as primary antibodies. We initially observed that murine macrophages express HSP65 when infected with either virulent or avirulent strains, a result which contradicts previous reports. Differential HSP expression consistent which virulence was observed between strains, with high levels of a 70kDa HSP (HSP70) only detected in virulent strains in vivo. This protein was not observed in virulent strains in the immunocompromised mouse or in vitro, suggesting induction by immunological stress. This protein was only poorly expressed in avirulent strains. A 65kDa protein was observed in all strains in vivo and in vitro, suggesting a shared epitope with HSP70. These results are consistent with the hypothesis that the induced expression of HSP70 in virulent strains of T. gondii by immunological stresses may provide protection for these strains against cell damage associated with invasion of the host, allowing the virulent strains to persist as tachyzoites without the requirement for the encystation observed in avirulent strains.
authors.affiliation: Molecular Parasitology Unit, Faculty of Science, University of Technology, Sydney, Gore Hill, NSW, Australia.
authors.forename: R E
authors.initials: RE
authors.lastname: Lyons
authors.forename: A M
authors.initials: AM
authors.lastname: Johnson
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000032:analysis; D005260:Female; D006360:Heat-Shock Proteins / Q000032:analysis / Q000096:biosynthesis*; D007121:Immunocompetence; D016867:Immunocompromised Host; D008264:Macrophages / Q000469:parasitology; D051379:Mice; D008807:Mice, Inbred BALB C; D015800:Protozoan Proteins / Q000032:analysis / Q000096:biosynthesis*; D014122:Toxoplasma / Q000378:metabolism* / Q000472:pathogenicity; D014124:Toxoplasmosis, Animal / Q000378:metabolism* / Q000469:parasitology; D016781:Toxoplasmosis, Cerebral / Q000378:metabolism / Q000469:parasitology; D014774:Virulence
chemical_list: D000911:Antibodies, Monoclonal; D000939:Epitopes; D006360:Heat-Shock Proteins; D015800:Protozoan Proteins