[First evaluation of the Variant haemoglobin analyzer (Bio-Rad)].
OBJECTIVE
The analytical performance of the first automated HPLC analyzer of haemoglobins A2 and Foetal is presented.


RESULTS
The analyzer shows an imprecision within-run between 0.66-2.7% and between-run 1.8-6.44. Correlation index with manual methods were r = 0.965 (p < 0.005) for Hb A2 and r = 0.999 (p < 0.0001) for Foetal Hb and the reference values of an adult population were established; Hb A2 (1.98-2.94%) and Hb F (0-0.95%). Several chromatograms of haemoglobinopathies are presented.


CONCLUSIONS
These results validate the method to resolve the increased demand of these parameters.
Aulesa
C
C
Laboratorio y Servicio de Hematología, Hospital Universitario Materno-Infantil del Valle d'Hebrón, Barcelona.
Comellas
J
J
Sentis
M
M
Ortega
J J
JJ
D000328:Adult; D002851:Chromatography, High Pressure Liquid; D005319:Fetal Hemoglobin / Q000032:analysis; D006441:Hemoglobin A / Q000032:analysis; D006454:Hemoglobins / Q000032:analysis*; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D007234:Infant, Premature; D012016:Reference Values; D012680:Sensitivity and Specificity; D017086:beta-Thalassemia / Q000097:blood
D006454:Hemoglobins; D006441:Hemoglobin A; D005319:Fetal Hemoglobin
Primera evaluación del analizador de hemoglobinas Variant (Bio-Rad).