Release of arachidonic acid via Ca2+ increase stimulated by pyrophosphonucleotides and bradykinin in mammary tumour cells.
The relationship between the increase of intracellular Ca2+ and the release of arachidonic acid by bradykinin and pyrophosphonucleotides was studied in cultured mammary tumour cells, MMT060562. Bradykinin, ATP, UTP and UDP induced an increase of intracellular Ca2+ and the release of arachidonic acid from phospholipids into the extracellular fluid. Release of arachidonic acid was also induced by the application of the Ca2+ ionophore, A23187. Liberation of arachidonic acid by bradykinin and ATP was reduced by mepacrine, a blocker of phospholipase A2 and W-7, a calmodulin antagonist. It is suggested that the increase in cytosolic Ca(2+)-induced release of arachidonic acid occurs through activation of calmodulin-dependent phospholipase A2.
Enomoto
K
K
Department of Physiology, Shimane Medical University, Japan.
Furuya
K
K
Yamagishi
S
S
Oka
T
T
Maeno
T
T
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism*; D001920:Bradykinin / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism*; D005260:Female; D008325:Mammary Neoplasms, Experimental / Q000378:metabolism*; D051379:Mice; D009712:Nucleotides, Cyclic / Q000494:pharmacology*; D018002:Receptors, Bradykinin / Q000502:physiology; D018048:Receptors, Purinergic P2 / Q000502:physiology; D058473:Receptors, Purinergic P2Y2; D015398:Signal Transduction / Q000502:physiology; D014407:Tumor Cells, Cultured / Q000502:physiology; D014530:Uridine Diphosphate / Q000494:pharmacology; D014544:Uridine Triphosphate / Q000494:pharmacology
D009712:Nucleotides, Cyclic; C546386:P2ry2 protein, mouse; D018002:Receptors, Bradykinin; D018048:Receptors, Purinergic P2; D058473:Receptors, Purinergic P2Y2; D016718:Arachidonic Acid; D014530:Uridine Diphosphate; D000255:Adenosine Triphosphate; D001920:Bradykinin; D002118:Calcium; D014544:Uridine Triphosphate