A new dominant selectable marker for genetic transformation; Hsp70-opd.
New P element plasmids containing the organophosphate-degrading gene opd as a dominant selectable marker were tested as transformation vectors in Drosophila melanogaster. One of these vectors was modified by the addition of the D. melanogaster mini-white gene as a comarker. When transformed individuals were identified using paraoxon selection for opd alone, results were similar to those obtained with mini-white. No false positives were recovered, however one strain contained the mini-white gene but inadequate resistance to survive our screening regimen due to a defective Hsp70-opd gene. Results suggest that Hsp70-opd is similar to mini-white for distinguishing transformed individuals but does not require time-consuming individual examination. Due to the mode of action of organophosphorus nerve agents, Hsp70-opd has potential as a selectable marker in numerous animals beside fruit flies.
Benedict
M Q
MQ
Centers for Disease Control and Prevention, Division of Parasitic Diseases, Atlanta, GA 30341, USA.
Salazar
C E
CE
Collins
F H
FH
D000818:Animals; D004331:Drosophila melanogaster / Q000235:genetics*; D005819:Genetic Markers; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics*; D007305:Insecticide Resistance / Q000235:genetics; D007306:Insecticides; D010261:Paraoxon; D010744:Phosphoric Monoester Hydrolases / Q000235:genetics / Q000378:metabolism; D010957:Plasmids; D014170:Transformation, Genetic
D005819:Genetic Markers; D018840:HSP70 Heat-Shock Proteins; D007306:Insecticides; C045290:phosphorylphosphatase; D010744:Phosphoric Monoester Hydrolases; D010261:Paraoxon