Feedforward control of agonist-induced Ca2+ signalling by protein kinase C in airway smooth muscle cells.
In isolated bovine tracheal smooth muscle cells, the potent and specific protein kinase C inhibitor GF 109203X caused an inhibition of methacholine-and histamine-induced Ca2+ mobilization and influx, indicating for the first time that protein kinase C activation induced by contractile agonists exerts a positive feedforward control of Ca2+ signalling by these agonists.
Hoiting
B H
BH
Department of Medicinal Chemistry and Molecular Pharmacology, University Center for Pharmacy, Groningen, Netherlands.
Kuipers
R
R
Elzinga
C R
CR
Zaagsma
J
J
Meurs
H
H
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002417:Cattle; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006632:Histamine / Q000494:pharmacology; D066298:In Vitro Techniques; D007211:Indoles / Q000494:pharmacology; D008301:Maleimides / Q000494:pharmacology; D016210:Methacholine Chloride / Q000494:pharmacology; D009130:Muscle, Smooth / Q000201:enzymology / Q000378:metabolism*; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects; D014132:Trachea / Q000166:cytology
D004791:Enzyme Inhibitors; D007211:Indoles; D008301:Maleimides; D016210:Methacholine Chloride; D006632:Histamine; D011493:Protein Kinase C; C070515:bisindolylmaleimide I; D002118:Calcium