[Biological monitoring of exposure to alkylating xenobiotics by determining them using a new analytical approach in complexes with hemoglobin, plasma proteins and mercapturic acids in urine. II. Acrylamide].
A new simple and prompt procedure for measuring acrylamide (propenamide, PA) in PA-derived mercapturic acid, such as N-acetyl-S-(2-propenamide)-L-cysteine (N-Ac-PAC), and in hemoglobin (Hb) and plasma protein adducts in the rats expose to PA was developed, by employing gas chromatography (GC). PA in mercapturic acids or proteins was liberated on high-temperature heating in an injector port during the working procedure of a GC capillary after preoxidation of sulfur atoms in PA-bound cysteine to a sulfoxide form with hydrogen peroxide and analyzed. This method resulted in 87% of PA in authentic N-Ac-PAC. The number of PA released from the protein adducts was proportional to the cumulative dose of PA given at a concentration of 1-75 mg/kg during 1-3 weeks. The alkylating level of Hb was approximately 65 times higher than that of plasma proteins and it was nearly 6.5% of its cumulative dose. The index of binding to Hb and the rate of its alkylation were 163 and 0.75.10(-3) liter/g-1/hour-1, respectively.
Hashimoto
K
K
Kanazawa University, Osaka, Japan.
Ivanov
V V
VV
Inomata
K
K
Kawai
T
T
Mizunuma
K
K
Klimatskaia
L G
LG
Fefelova
Iu A
IuA
D000111:Acetylcysteine / Q000652:urine*; D020106:Acrylamide; D000178:Acrylamides / Q000737:chemistry* / Q000633:toxicity; D000477:Alkylating Agents / Q000737:chemistry* / Q000633:toxicity; D000818:Animals; D001798:Blood Proteins / Q000737:chemistry*; D002849:Chromatography, Gas; D006454:Hemoglobins / Q000737:chemistry*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D015262:Xenobiotics / Q000737:chemistry / Q000633:toxicity
D000178:Acrylamides; D000477:Alkylating Agents; D001798:Blood Proteins; D006454:Hemoglobins; D015262:Xenobiotics; D020106:Acrylamide; D000111:Acetylcysteine
Biologicheskiĭ monitoring vozdeĭstviia alkiliruiushchikh ksenobiotikov putem ikh opredeleniia s pomoshch'iu novogo analiticheskogo podkhoda v soedineniiakh s gemoglobinom, belkami plazmy i merkapturovykh kislotakh mochi. II. Akrilamid.