Analysis and localization of cyclophilin A found in the virions of human immunodeficiency virus type 1 MN strain.
Previous reports have shown that cyclophilin A (CyPA) is found to be specifically associated with human immunodeficiency virus type-1 (HIV-1) virions and is required for infectivity (Franke et al. Nature 372:359; Thali et al. Nature 372:363). We have examined CyPA associated with HIV-1MN virions. Virions from infected human lymphoid cells were analyzed by high-pressure liquid chromatography (HPLC), protein sequence, and immunoblot analysis. At least three forms of CyPA were found: an unmodified form, an N-terminally modified form, and an N-terminally modified form that migrates as a larger isoform on a reducing-SDS polyacrylamide gel. Using a protease digestion procedure, CyPA that is associated with virions was found to be located inside the viral membrane. Similar examination of SIVMne produced by HUT-78 human T cells did not detect specific incorporation of CyPA into SIV virions. Our results are consistent with the role of CyPA acting early in the infectious process of HIV-1.
Ott
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AIDS Vaccine Program, National Cancer Institute, Frederick Cancer Research and Development Center, Maryland 21702-1201, USA.
Coren
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LV
Johnson
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DG
Sowder
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RC
Arthur
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LO
Henderson
L E
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D000591:Amino Acid Isomerases / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D000596:Amino Acids / Q000032:analysis; D002352:Carrier Proteins / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D015497:HIV-1 / Q000737:chemistry* / Q000254:growth & development / Q000378:metabolism; D006801:Humans; D015151:Immunoblotting; D019696:Peptidylprolyl Isomerase; D013381:Subtilisins; D014759:Viral Envelope Proteins / Q000032:analysis / Q000378:metabolism
D000596:Amino Acids; D002352:Carrier Proteins; D014759:Viral Envelope Proteins; D013381:Subtilisins; D000591:Amino Acid Isomerases; D019696:Peptidylprolyl Isomerase