Rapid identification of Acremonium lolii and Acremonium coenophialum endophytes through arbitrarily primed PCR.
Using a random decamer 5'-CCGAGGTGAC-3' in an arbitrarily primed PCR, similar band patterns were observed between Acremonium lolii and A. coenophialum DNA, which were somewhat different from those formed by other fungal DNA. Despite sharing bands of around 0.7, 0.9 and 2.1 kb, A. lolii can be distinguished from A. coenophialum by the presence of an additional band at around 0.5 kb in the arbitrarily primed PCR.
Liu
D
D
Victorian Institute of Animal Science, Attwood, Australia.
van Heeswijck
R
R
Latch
G
G
Leonforte
T
T
Panaccio
M
M
Langford
C
C
Cunningham
P
P
Reed
K
K
D000164:Acremonium / Q000235:genetics* / Q000302:isolation & purification; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D015342:DNA Probes / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics; D008129:Lolium / Q000382:microbiology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*
D017931:DNA Primers; D015342:DNA Probes; D004271:DNA, Fungal