title: Binding of the sigma 70 protein to the core subunits of Escherichia coli RNA polymerase, studied by iron-EDTA protein footprinting.
abstract: We have used a nonspecific protein cleaving reagent to map the interactions between subunits of the multisubunit enzyme RNA polymerase (Escherichia coli). We developed suitable conditions for using an untethered Fe-EDTA reagent, which does not bind significantly to proteins. Comparison of the cleaved fragments of the subunits from the core enzyme (alpha 2 beta beta') and the holoenzyme (core+sigma 70) shows that absence of the sigma 70 subunit is associated with the appearance of several cleavage sites on the subunits beta (within 10 residues of sequence positions 745, 764, 795, and 812) and beta' (within 10 residues of sequence positions 581, 613, and 728). A cleavage site near beta residue 604 is present in the holoenzyme but absent in the core, demonstrating that a conformational change occurs when sigma 70 binds. No differences are observed for the alpha subunit.
authors.affiliation: Department of Chemistry, University of California, Davis 95616, USA.
authors.forename: D P
authors.initials: DP
authors.lastname: Greiner
authors.forename: K A
authors.initials: KA
authors.lastname: Hughes
authors.forename: A H
authors.initials: AH
authors.lastname: Gunasekera
authors.forename: C F
authors.initials: CF
authors.lastname: Meares
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D012321:DNA-Directed RNA Polymerases / Q000737:chemistry*; D004492:Edetic Acid / Q000737:chemistry; D004926:Escherichia coli / Q000201:enzymology*; D007501:Iron / Q000737:chemistry; D008969:Molecular Sequence Data; D010449:Peptide Mapping; D011485:Protein Binding; D012808:Sigma Factor / Q000737:chemistry*
chemical_list: D012808:Sigma Factor; D004492:Edetic Acid; D007501:Iron; D012321:DNA-Directed RNA Polymerases