Mutational analysis of influenza B virus RNA transcription in vitro.
The roles of the 3'- and 5'-terminal nucleotides and the panhandle structure of influenza B virus virion RNA were analyzed in vitro by transcription of model RNA templates with influenza B virus RNA polymerase. The results suggest that the stability of the panhandle and breathing of the extreme ends of the panhandle are important factors for efficient transcription. Influenza B virus polymerase appears to be more tolerant of mutations in the panhandle structure than influenza A virus polymerase. This is consistent with the greater degree of heterogeneity observed naturally in the 3'-terminal nucleotides of the virion RNA of influenza B virus than in influenza A virus.
Lee
Y S
YS
Institute of Biological Sciences, Hanhyo Institutes of Technology, Kyungki-Do, South Korea.
Seong
B L
BL
D001483:Base Sequence; D001665:Binding Sites; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D006801:Humans; D009980:Influenza A virus / Q000235:genetics; D009981:Influenza B virus / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D009690:Nucleic Acid Conformation; D012367:RNA, Viral / Q000235:genetics*; D013329:Structure-Activity Relationship; D014158:Transcription, Genetic
D012367:RNA, Viral; D012321:DNA-Directed RNA Polymerases