Ornithine decarboxylase and tyrosine kinase activity in juvenile polyps of childhood.
Juvenile polyps (JP) are the most common colonic tumor in children. Although considered benign, malignant transformation has been reported in JP. Ornithine decarboxylase (ODC) and tyrosine kinase (TyK) enzymes are markers for a rapid cell proliferation index. DNA aneuploidy score and p53 gene expression are late malignant changes seen in patients with colon cancer. In this study, we investigated ODC and TyK activities as well as DNA aneuploidy score and p53 expression in juvenile polyps compared with the adjacent normal colonic mucosa. Results showed that ODC was significantly increased in JP compared with the adjacent normal colonic mucosa. TyK activity was increased in 3/5 polyps and decreased in 2/5 polyps compared with the mucosa. Mean TyK activity was higher in JP compared with normal mucosa but did not reach significance (707 and 632 pmol/mg pmol, respectively). Moreover, changes in phosphorylization of TyK proteins was also observed in JP but not in normal mucosa. JP had a normal DNA aneuploidy score and showed no expression of p53 gene. We conclude that JP do not express p53 gene and aneuploidy but had higher activity of ODC and TyK enzymes, suggesting a higher stage of cell proliferation.
Elitsur
Y
Y
Department of Pediatrics, Marshall University, Huntington, West Virginia 25701-0195, USA.
Koh
S J
SJ
Moshier
J A
JA
Dosescu
J
J
Tureaud
J
J
Majumdar
A P
AP
D000293:Adolescent; D000782:Aneuploidy; D002648:Child; D002675:Child, Preschool; D003106:Colon / Q000201:enzymology; D003111:Colonic Polyps / Q000201:enzymology* / Q000235:genetics; D004273:DNA, Neoplasm / Q000235:genetics; D015870:Gene Expression; D016158:Genes, p53; D006801:Humans; D007223:Infant; D007413:Intestinal Mucosa / Q000201:enzymology; D009955:Ornithine Decarboxylase / Q000378:metabolism*; D011505:Protein-Tyrosine Kinases / Q000378:metabolism*
D004273:DNA, Neoplasm; D011505:Protein-Tyrosine Kinases; D009955:Ornithine Decarboxylase