title: Presence of antibodies to replication protein A in some patients with systemic lupus erythematosus (SLE).
abstract: Presence of antibodies directed against replication protein A (RPA), a DNA binding protein complex composed of three subunits (RPA-70, RPA-32 and RPA-14) was investigated among patients with SLE and other autoimmune diseases using immunoblot analysis to RPA-70 and RPA-32 recombinant proteins. Anti-RPA antibodies were found in two out of 108 sera from SLE patients, one of them showing reactivity against RPA-32 and RPA-70 and the other reacting only against RPA-32. Sera from 108 patients with other autoimmune disorders as well as from 42 healthy control individuals were negative. Thus, the frequency of these antibodies in SLE is estimated to be 2-3%. The study demonstrates that RPA is one target more of the wide array of autoantigens that elicit an immune response in SLE. The presence of anti-RPA autoantibodies seems to be circumscribed to a small number of patients with SLE.
authors.affiliation: Servicio de Immunologia, Hospital Universitario Virgen del Rocio, Servicio Andaluz de Salud, Sevilla, Spain.
authors.forename: R
authors.initials: R
authors.lastname: Garcia-Lozano
authors.forename: I
authors.initials: I
authors.lastname: Wichmann
authors.forename: A
authors.initials: A
authors.lastname: Garcia
authors.forename: J
authors.initials: J
authors.lastname: Sanchez-Roman
authors.forename: F
authors.initials: F
authors.lastname: Gonzalez-Escribano
authors.forename: A
authors.initials: A
authors.lastname: Nuñez-Roldan
mesh_terms: D000328:Adult; D000368:Aged; D001323:Autoantibodies / Q000097:blood*; D004268:DNA-Binding Proteins / Q000276:immunology*; D005260:Female; D006367:HeLa Cells; D006801:Humans; D015151:Immunoblotting; D008180:Lupus Erythematosus, Systemic / Q000097:blood / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D051716:Replication Protein A
chemical_list: D001323:Autoantibodies; D004268:DNA-Binding Proteins; C497167:RPA1 protein, human; D051716:Replication Protein A