title: Isolation of a bovine uterotropic placental factor and development of an enzyme immunoassay in peripheral serum.
abstract: Bovine uterotropic placental factor (bUTPF) was obtained from bovine term placentae that had been homogenized and extracted in acidic medium. The extracts were chromatographed on Sephadex G-75, a fraction named native bUTPF was obtained, and an antiserum was generated. The native bUTPF was chromatographed on Sephacryl S-300 HR, and a 230-kDa fraction was obtained. A competitive enzyme immunoassay (EIA) was developed to determine bUTPF levels during gestation. This EIA was linear between 0 and 80 micrograms/ml (with a detection limit of 0.2 microgram/ml) and showed intraassay and interassay coefficients of variation of 11.2% and 15.5%, respectively. Bovine UTPF serum levels during gestation were determined by means of a cross-sectional study between the first month of gestation and delivery. Maximal serum levels of bUTPF were found during the first month of gestation: 7.1 +/- 1.9 micrograms/ml (p < 0.05). In another cross-sectional study, blood samples were collected from 105 cows between 8 and 25 days postinsemination (day of insemination = Day 0). Levels of bUTPF were found to be significantly higher (p < 0.05) by Day 17 compared to Day 0 (5.8 +/- 2.2 micrograms/ml vs. 1.2 +/- 0.7 micrograms/ml). We conclude that bUTPF is detected by EIA in maternal peripheral serum early during gestation in the bovine species.
authors.affiliation: Instituto de Investigaciones Materno Infantil (IDIMI), Facultad de Medicina, Universidad de Chile, Santiago, Chile.
authors.forename: G M
authors.initials: GM
authors.lastname: Iñiguez
authors.forename: M
authors.initials: M
authors.lastname: Galanti
authors.forename: M A
authors.initials: MA
authors.lastname: Boric
authors.forename: G
authors.initials: G
authors.lastname: Ferrando
authors.forename: B
authors.initials: B
authors.lastname: Muñoz
authors.forename: F
authors.initials: F
authors.lastname: Beas
mesh_terms: D000818:Animals; D001667:Binding, Competitive; D015153:Blotting, Western; D002417:Cattle / Q000097:blood*; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005779:Immunodiffusion; D007124:Immunoenzyme Techniques; D010920:Placenta / Q000737:chemistry; D010926:Placental Hormones / Q000097:blood* / Q000302:isolation & purification; D011247:Pregnancy; D012016:Reference Values; D013997:Time Factors
chemical_list: D010926:Placental Hormones; C004230:placental uterotrophic factor