title: Degradation profile and identification of the major degradation products of dobupride under several conditions by GC/MS and HPLC-particle beam/MS.
abstract: The effect of pH, light, temperature and oxygen on the stability of dobupride (1), a novel gastroprokinetic drug, has been studied, storing the sample in the solid state and as a solution in methanol-water. The main forced degradation products have been identified by means of techniques such as GC/MS and HPLC-particle beam/MS, and two major degradation pathways have been characterized. One degradation route involves the loss of chlorine, yielding 4-amino-2-butoxy-N-[1-(1,3-dioxolan-2-ylmethyl)piperid -4-yl]benzamide (4) as the major degradation product. The second pathway results from cleavage of the piperidine-amide bond, producing 4-amino-2-butoxy-5-chlorobenzamide (2) as the major degradation product. Under the studied conditions, except when exposed to direct light in solution, dobupride has been shown to be very stable: after 5 months storage, the benzamide 2 (second pathway) was the only product identified (less than 0.5%). However, when dobupride in solution is exposed to natural or artificial sunlight, degradation is very fast, and after 7 days only 5% of the unchanged product remains. Under these circumstances, the main degradation route is the first one, with compound 4 being the most abundant degradation product, and compound 2 only being detectable in small amounts.
authors.affiliation: Department of Analysis R&D, Laboratorios Almirall, S.A., Barcelona, Spain.
authors.forename: V
authors.initials: V
authors.lastname: Segarra
authors.forename: F
authors.initials: F
authors.lastname: Carrera
authors.forename: J L
authors.initials: JL
authors.lastname: Fabregas
authors.forename: J
authors.initials: J
authors.lastname: Claramunt
mesh_terms: D001549:Benzamides / Q000032:analysis* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D004355:Drug Stability; D008401:Gas Chromatography-Mass Spectrometry; D005765:Gastrointestinal Agents / Q000032:analysis* / Q000737:chemistry; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008027:Light; D013058:Mass Spectrometry; D010084:Oxidation-Reduction
chemical_list: D001549:Benzamides; D005765:Gastrointestinal Agents; C098101:dobupride