A 19F NMR study of lomefloxacin in human erythrocytes and its interaction with hemoglobin.
19F NMR spectroscopy of a model fluoroquinolone, lomefloxacin, in an erythrocyte suspension showed separate resonances for the intra- and extra-cellular compartments. The intra-cellular peak revealed significant line broadening of the fluorine signals of lomefloxacin. Line broadening also occurred in the presence of oxyhemoglobin (HbO2), hematin, globin and iron. This evidence indicated that lomefloxacin interacted with these compounds; however, ultrafiltration experiments indicated that there was only weak binding (5%) of lomefloxacin to HbO2. 19F and 31P NMR spectroscopy revealed that lomefloxacin may compete with 2,3-diphosphoglycerate for its binding site on HbO2. An apparent partition coefficient of 1.90 +/- 0.15 was observed for lomefloxacin in human erythrocytes, utilizing LC analysis.
Knaub
S R
SR
Department of Pharmaceutical Chemistry, University of Kansas, Lawrence 66045, USA.
Priston
M J
MJ
Morton
M D
MD
Slechta
J D
JD
Vander Velde
D G
DG
Riley
C M
CM
D000890:Anti-Infective Agents / Q000097:blood*; D001665:Binding Sites; D004492:Edetic Acid / Q000494:pharmacology; D004912:Erythrocytes / Q000737:chemistry*; D024841:Fluoroquinolones; D006454:Hemoglobins / Q000378:metabolism*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D011485:Protein Binding; D015363:Quinolones / Q000097:blood* / Q000378:metabolism
D000890:Anti-Infective Agents; D024841:Fluoroquinolones; D006454:Hemoglobins; D015363:Quinolones; D004492:Edetic Acid; C053091:lomefloxacin