Specific interferon-gamma producing CD4+ and CD8+ T cells after autologous EBV-B stimulation: the necessity of restimulation.
The response of T cells to produce interferon-gamma, to proliferate and to become cytotoxic after specific stimulation with low dose (2%) autologous EBV-B cells was investigated in 15 EBV seropositive and five seronegative patients. A significantly higher number of interferon-gamma producing cells (56 +/- 24 per 10(5) T cells) were found in a spot ELISA in EBV positive than in EBV negative patients (7 +/- 2 spots, P < 0.01) and it was only found with restimulation after 5-12 days of primary culture. No correlation was found between the extent of interferon-gamma production, cytotoxicity or proliferation. Specificity of EBV-induced interferon-gamma production was demonstrated by comparison of the response to allogeneic EBV-B cells or IL-2 in the restimulation phase. The response was stronger in CD8+ T cells than in CD4+ T cells and could be blocked in the restimulation phase with HLA class I and class II antiserum, respectively.
Vooijs
W C
WC
Department of Immunohaematology, University Hospital Utrecht, The Netherlands.
Nootebos
M G
MG
Wernert
H
H
de Gast
G C
GC
D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D003602:Cytotoxicity, Immunologic; D006680:HLA Antigens / Q000276:immunology; D004854:Herpesvirus 4, Human / Q000276:immunology*; D006801:Humans; D066298:In Vitro Techniques; D007371:Interferon-gamma / Q000096:biosynthesis*; D008213:Lymphocyte Activation; D013997:Time Factors
D006680:HLA Antigens; D007371:Interferon-gamma