Entamoeba histolytica axenic growth improvement by ox bile.
Axenic Entamoeba histolytica cultures, grown in PEHPS medium, showed increasing yields and growth velocity when added with 0.03 to 0.6 g/l gallbladder ox bile, while higher doses were toxic for amebas. The highest density (505 +/- 29 x 10(3) trophozoites/ml) and shortest duplication time (Dt = 10.74 +/- 0.2 h) corresponded to cultures added with 0.24 g bile/l. Their yields were 1.74 and 3.34 times higher, respectively, than those which were reached by the non-added PEHPS controls and by growth of amebas in TYI-S-33 medium. Between 72 and 96 h of incubation a noticeable increase in trophozoite density was observed in cultures added with 0.24 g/1 bile among controls. At 72 h yields of bile-added cultures inoculated with 5, 10 and 20 x 10(3) amebas/ml increased in function of the inoculum. The improved growth of E. histolytica by adding 0.24 g/l ox bile to culture medium suggests that bile has compounds are essential for amebas.
Mata-Cárdenas
B D
BD
División de Biología Celular y Molecular, Centro de Investigación Biomédica del Noreste, Instituto Mexicano del Seguro Social, Monterrey, N.L., México.
Vargas-Villarreal
J
J
Martínez-Rodríguez
H G
HG
Said-Fernández
S
S
D000818:Animals; D001646:Bile / Q000378:metabolism*; D002417:Cattle; D003470:Culture Media; D004748:Entamoeba histolytica / Q000187:drug effects* / Q000254:growth & development*; D005856:Germ-Free Life
D003470:Culture Media