Elastic fibre assembly: macromolecular interactions.
To investigate the mechanisms behind elastic fibre assembly, we studied the molecular interactions between elastin and microfibrillar components using solid-phase binding assays. Fibrillin 1, purified from tissue using reductive-saline extraction, showed no binding to microfibril-associated glycoprotein (MAGP) or tropoelastin. MAGP, however, was found to bind specifically to tropoelastin in a divalent-cation independent manner. Antibody inhibition studies indicated that the C-terminus of tropoelastin defined the interactive site with MAGP. MAGP and fibrillin were also substrates for transglutaminase, which may provide an important mechanism for stabilizing microfibrillar structure. In other studies we found that a major cross-linking region in elastin is formed through the association of domains encoded by exons 10, 19 and 25 of tropoelastin and that the three chains are joined together by one desmosine and two lysinonorleucine cross-links.
Mecham
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RP
Department of Cell Biology and Physiology, Washington University School of Medicine, St. Louis, MO 63110, USA.
Broekelmann
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T
Davis
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Gibson
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MA
Brown-Augsburger
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P
D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D003285:Contractile Proteins / Q000378:metabolism*; D004547:Elastic Tissue / Q000378:metabolism*; D004549:Elastin / Q000378:metabolism*; D016326:Extracellular Matrix Proteins; D000071838:Fibrillin-1; D000071837:Fibrillins; D006801:Humans; D008840:Microfilament Proteins / Q000378:metabolism*; D008969:Molecular Sequence Data; D011485:Protein Binding; D008249:Protein-Lysine 6-Oxidase / Q000378:metabolism; D000072260:RNA Splicing Factors; D013379:Substrate Specificity; D011503:Transglutaminases / Q000378:metabolism; D014333:Tropoelastin / Q000378:metabolism; D054392:Adipokines
D003285:Contractile Proteins; D004549:Elastin; D016326:Extracellular Matrix Proteins; D000071838:Fibrillin-1; D000071837:Fibrillins; D008840:Microfilament Proteins; D008249:Protein-Lysine 6-Oxidase; D000072260:RNA Splicing Factors; D011503:Transglutaminases; D014333:Tropoelastin; C000605722:FBN1 protein, human; D054392:Adipokines; C016598:microfibrillar protein