title: Molecular characterization of the 11th RNA segment from human group C rotavirus.
abstract: The complete nucleotide sequence of genome segment 11 from the noncultivatable, human group C rotavirus (Bristol strain) was determined. Comparison of the nucleotide sequence of the segment termini with the consensus 5' and 3' terminal noncoding sequences of the human group C rotavirus genome revealed characteristic 5' and 3' sequences. Human group C rotavirus genome segment 11 is 613 bp long and encodes a single open reading frame of 450 nucleotides (150 amino acids) starting at nucleotide 39 and terminating at nucleotide 489, leaving a long 3' untranslated region of 124 nucleotides. The predicted translation product has a calculated molecular weight of 17.7 kD and contains four potential N-linked glycosylation sites. No significant homologies to other viral proteins were found in database searches. Hydropathy analysis predicted the human group C rotavirus genome segment 11 translation product has a hydrophilic carboxy terminus (amino acids 54-150) and a hydrophobic amino terminus (amino acids 1-53) that can be further subdivided into three short hydrophobic sequences--H1, H2, and H3. These features are analogous to the integral membrane glycoprotein NSP4 encoded by group A rotavirus gene 10.
authors.affiliation: University Medical School, Southampton General Hospital, United Kingdom.
authors.forename: Y
authors.initials: Y
authors.lastname: Deng
authors.forename: P A
authors.initials: PA
authors.lastname: Fielding
authors.forename: P R
authors.initials: PR
authors.lastname: Lambden
authors.forename: E O
authors.initials: EO
authors.lastname: Caul
authors.forename: I N
authors.initials: IN
authors.lastname: Clarke
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D001665:Binding Sites; D004279:DNA, Viral; D006031:Glycosylation; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000235:genetics*; D012401:Rotavirus / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics
chemical_list: D004279:DNA, Viral; D012367:RNA, Viral; D014764:Viral Proteins