Determination of some non-steroid anti-inflammatory drugs by capillary isotachophoresis.
The isotachophoretic (ITP) behaviour and separation of the anti-inflammatory drugs kebuzone (KB), tribuzone (TB) and phenylbutazone (PB) was studied in the operational system of HCl/His (leading electrolyte, LE) and 4-nitrophenol (terminating electrolyte, TE). The effective mobilities were 19.4 x 10(-9) m2 V-1 s-1 for KB, 18.1 x 10(-9) m2 V-1 s-1 for TB and 18.9 x 10(-9) m2 V-1 s-1 for PB when using an optimised system with 10 mM HCl + 40 mM His (pH 6.63) as LE and 10 mM 4-nitrophenol as TE. The calibration graphs were rectilinear (r = 0.9982-0.9996) in the range 20 to 600 mumol 1-1 of KB, TB or PB. The ITP method was used for determining the content of KB, TB or PB in mass-produced pharmaceuticals as tablets, coated tablets, injections, and ointments. The results of the ITP determination were in good agreement with those of standard pharmacopoeial methods.
Pospísilová
M
M
Faculty of Pharmacy, Charles University, Hradec Králové, Czech Republic.
Jokl
V
V
Polásek
M
M
Mokrá
J
J
D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000032:analysis*; D004573:Electrolytes; D004586:Electrophoresis / Q000379:methods*; D006851:Hydrochloric Acid / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D009596:Nitrophenols / Q000737:chemistry; D010653:Phenylbutazone / Q000031:analogs & derivatives* / Q000032:analysis*
D000894:Anti-Inflammatory Agents, Non-Steroidal; D004573:Electrolytes; D009596:Nitrophenols; C100280:kebuzone; C100215:tribuzone; D010653:Phenylbutazone; D006851:Hydrochloric Acid; C024836:4-nitrophenol