title: Expression of an unusual Bw4 epitope by a subtype of HLA-B8 [B*0802].
abstract: The primary structure of a variant HLA-B8 antigen has been determined by cDNA cloning and sequencing. The variant, B*0802 differs, from the common B*0801 subtype at positions 77-83 of the alpha 1 helix that determine the Bw4 and Bw6 public epitopes. Whereas B*0801 has the common Bw6 motif, B*0802 has the Bw4 motif found in B*13 and B*44 allotypes. Serological analysis of B cell lines expressing B*0802 and of a B*0802 transfectant made with the HLA-A,B negative cell line 721.221 shows that B*0802 reacts with Bw4-specific antibodies, but at a level much lower than expected for Bw4 positive HLA-B allotypes.
authors.affiliation: Department of Structural Biology, Stanford University, California, USA.
authors.forename: K L
authors.initials: KL
authors.lastname: Arnett
authors.forename: E J
authors.initials: EJ
authors.lastname: Adams
authors.forename: J E
authors.initials: JE
authors.lastname: Gumperz
authors.forename: C
authors.initials: C
authors.lastname: Darke
authors.forename: S G
authors.initials: SG
authors.lastname: Marsh
authors.forename: K
authors.initials: K
authors.lastname: Gelsthorpe
authors.forename: P
authors.initials: P
authors.lastname: Parham
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology; D002461:Cell Line, Transformed; D000939:Epitopes / Q000737:chemistry / Q000276:immunology*; D005260:Female; D015235:HLA-B Antigens / Q000737:chemistry / Q000276:immunology*; D015795:HLA-B8 Antigen / Q000737:chemistry / Q000276:immunology*; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D014407:Tumor Cells, Cultured
chemical_list: D000911:Antibodies, Monoclonal; D000939:Epitopes; D015235:HLA-B Antigens; D015795:HLA-B8 Antigen; C042388:HLA-Bw4 antigen