title: Immunolocalization of aquaporin CHIP in the guinea pig inner ear.
abstract: Aquaporin CHIP (AQP-CHIP) is a water channel protein previously identified in red blood cells and water transporting epithelia. The inner ear is an organ of hearing and balance whose normal function depends critically on maintenance of fluid homeostasis. In this study, AQP-CHIP, or a close homologue, was found in specific cells of the inner ear, as assessed by immunocytochemistry with the use of affinity-purified polyclonal antibodies against AQP-CHIP.AQP-CHIP was predominantly found in fibrocytes in close association with bone, including most of the cells lining the bony labyrinth and in fibrocytes lining the endolymphatic duct and sac. AQP-CHIP-positive cells not directly apposing bone include cells under the basilar membrane, some type III fibrocytes of the spiral ligament, fibrocytes of the spiral limbus, and the trabecular perilymphatic tissue extending from the membranous to the bony labyrinth. AQP-CHIP was also found in the periosteum of the middle ear and cranial bones, as well as in chondrocytes of the oval window and stapes. The distribution of AQP-CHIP in the inner ear suggests that AQP-CHIP may have special significance for maintenance of bone and the basilar membrane, and for function of the spiral ligament.
authors.affiliation: Division of Health Sciences and Technology, Harvard-Massachusetts Institute of Technology, Boston, USA.
authors.forename: K M
authors.initials: KM
authors.lastname: Stanković
authors.forename: J C
authors.initials: JC
authors.lastname: Adams
authors.forename: D
authors.initials: D
authors.lastname: Brown
mesh_terms: D000818:Animals; D051398:Aquaporin 1; D020346:Aquaporins; D001489:Basilar Membrane / Q000378:metabolism; D003051:Cochlea / Q000378:metabolism; D007758:Ear, Inner / Q000378:metabolism*; D004432:Ear, Middle / Q000378:metabolism; D004711:Endolymphatic Duct / Q000378:metabolism; D004712:Endolymphatic Sac / Q000378:metabolism; D006168:Guinea Pigs; D007150:Immunohistochemistry; D007473:Ion Channels / Q000378:metabolism*; D010498:Perilymph / Q000378:metabolism; D014018:Tissue Distribution
chemical_list: D020346:Aquaporins; D007473:Ion Channels; D051398:Aquaporin 1