Cattle serologically positive for Brucella abortus have antibodies to B. abortus Cu-Zn superoxide dismutase.
In this study, we demonstrated by a Cu-Zn superoxide dismutase-specific enzyme-linked immunoassay that cattle that are serologically positive for Brucella abortus have serum immunoglobulin G antibodies to B. abortus Cu-Zn superoxide dismutase. The specificity of the antibody reactivity was confirmed by Western blot (immunoblot) analysis with B. abortus salt-extractable proteins containing native Cu-Zn superoxide dismutase and with recombinant B. abortus Cu-Zn superoxide dismutase. The results represent a first step in the direction of the development of a multiprotein diagnostic reagent for bovine brucellosis.
Tabatabai
L B
LB
National Animal Disease Center, Agricultural Research Service, U.S. Department of Agriculture, Ames, Iowa, USA.
Hennager
S G
SG
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D000918:Antibody Specificity; D002003:Brucella abortus / Q000201:enzymology / Q000276:immunology*; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay; D007074:Immunoglobulin G / Q000097:blood; D013482:Superoxide Dismutase / Q000276:immunology*
D000907:Antibodies, Bacterial; D007074:Immunoglobulin G; D013482:Superoxide Dismutase