A 3.1-Mb YAC contig within the Werner syndrome region, on the short arm of human chromosome 8.
The locus for Werner syndrome (WRN) has been localized to human chromosome 8p21 --> p12, close to the anonymous marker D8S339. A 3.1-Mb contig of yeast artificial chromosomes (YACs) was assembled around D8S339. Results from analyses of somatic cell hybrids, FISH, and physical mapping suggest the following loci order: tel-NEFL-D8S131-D8S339-[D8S540/GSR]-D8S124 -D8S259-D8S87-FGFR1-cen. Close physical linkage between D8S540 and GSR was established within a DNA fragment of 200 kb. These two loci are not more than 400 kb from D8S339. In addition, D8S339, D8S540, D8S124, and GSR are within 1.1 Mb. These data establish a primary physical map of the Werner syndrome region and identify useful YAC clones for the isolation of new markers and of the corresponding gene.
Chaffanet
M
M
Laboratoire d'Oncologie Moléculaire, U.119 INSERM, Marseille, France.
Imbert
A
A
Adélaïde
J
J
Le Paslier
D
D
Wagner
M J
MJ
Wells
D E
DE
Birnbaum
D
D
Pébusque
M J
MJ
D002874:Chromosome Mapping; D018244:Chromosomes, Artificial, Yeast; D002898:Chromosomes, Human, Pair 8; D005819:Genetic Markers; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D015183:Restriction Mapping; D014898:Werner Syndrome / Q000235:genetics*
D005819:Genetic Markers