Frequent development of murine T-cell lymphomas with TcR alpha/beta+, CD4-/8- phenotype after implantation of human inflammatory breast cancer cells in BALB/c nude mice.
Tumors developed quite frequently in some of the visceral organs, including spleen and liver, in BALB/c nude mice upon subcutaneously xenografting surgical specimens from five different inflammatory breast cancer patients. All of these tumors developed within two and a half months to one year after the subcutaneous inoculation of surgical specimens. From these tumors, five independent transplantable tumors, including tMK-2, tHK-1, tYK-1, tYK-2 and tTY-1 have been established. Chromosome analysis, morphologic studies by light and electron microscopy and phenotype analysis indicated that these tumors are of mouse origin. The tMK-2 tumor was highly metastatic to the spleen and liver when it was subcutaneously transplanted into the right scapular region. In addition, the region where the tMK-2 tumor cells were subcutaneously inoculated showed an apparently inflammatory process represented by erythema. After subcutaneous inoculation into the right scapular region, tHK-1, tYK-1, 2, and tTY-1 tumors also metastasized to some of the visceral organs, including spleen and liver. From these tumors, in vitro cell lines were established. The cells grew in a stromal-cell dependent manner under in vitro culture conditions. The cells were again tumorigenic at the inoculated region and metastasized to various organs, including liver and spleen, of BABL/c nude mice. Histological examination revealed that the tumors showed features of malignant lymphoma. Phenotypically, these five tumors expressed early T lymphocyte markers as revealed by anti-mouse anti-TcR alpha/beta, anti-CD3, CD4 and CD8 monoclonal antibodies. To our knowledge, these cell lines are the first T-cell lines showing the phenotype of extrathymically differentiated T-cells in the liver.
Wakasugi
H
H
Section of Studies of Host-Immune Response, National Cancer Center Research Institute, Tokyo.
Koyama
K
K
Gyotoku
M
M
Yoshimoto
M
M
Hirohashi
S
S
Sugimura
T
T
Terada
M
M
D000818:Animals; D000951:Antigens, Neoplasm / Q000032:analysis*; D001943:Breast Neoplasms / Q000276:immunology / Q000473:pathology*; D015704:CD4 Antigens / Q000032:analysis; D016827:CD8 Antigens / Q000032:analysis; D002277:Carcinoma / Q000276:immunology / Q000473:pathology*; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D018920:Coculture Techniques; D003238:Connective Tissue / Q000473:pathology; D004273:DNA, Neoplasm / Q000032:analysis; D004890:Erythema / Q000209:etiology; D005260:Female; D006801:Humans; D016130:Immunophenotyping; D007249:Inflammation; D007279:Injections, Subcutaneous; D007621:Karyotyping; D008099:Liver / Q000473:pathology; D008113:Liver Neoplasms / Q000473:pathology; D016399:Lymphoma, T-Cell / Q000209:etiology* / Q000235:genetics / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009362:Neoplasm Metastasis; D009368:Neoplasm Transplantation / Q000009:adverse effects* / Q000276:immunology; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000032:analysis; D013045:Species Specificity; D013154:Spleen / Q000473:pathology; D013160:Splenic Neoplasms / Q000473:pathology; D014183:Transplantation, Heterologous / Q000009:adverse effects* / Q000276:immunology; D014407:Tumor Cells, Cultured / Q000473:pathology / Q000637:transplantation
D000951:Antigens, Neoplasm; D015704:CD4 Antigens; D016827:CD8 Antigens; D004273:DNA, Neoplasm; D016693:Receptors, Antigen, T-Cell, alpha-beta