Tissue-nonspecific alkaline phosphatase participates in the establishment and growth of feather germs in embryonic chick skin cultures.
Alkaline phosphatase activity is present in the mesoderm of embryonic chick skin and becomes spatially restricted to the dermal condensation of the developing feather germs. Inhibitors to tissue-nonspecific (liver/bone/kidney), but not intestinal alkaline phosphatase inhibit the establishment and growth of feather germs in cultured skins. A window of maximum sensitivity to the inhibitor was observed to be the first day of culture when early development and establishment of pattern takes place. The cDNA for the avian tissue-nonspecific alkaline phosphatase was cloned and sequenced, and Southern analysis revealed a single copy of this gene in the avian genome. Northern analysis revealed that a 2.8 kb transcript for this form of alkaline phosphatase is present in developing skin.
Crawford
K
K
Cancer Research Center, La Jolla Cancer Research Foundation, California 92037, USA.
Weissig
H
H
Binette
F
F
Millán
J L
JL
Goetinck
P F
PF
D000469:Alkaline Phosphatase / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002642:Chick Embryo / Q000196:embryology*; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis; D005241:Feathers / Q000196:embryology* / Q000201:enzymology; D006709:Homoarginine / Q000494:pharmacology; D007978:Levamisole / Q000494:pharmacology; D008969:Molecular Sequence Data; D009924:Organ Culture Techniques; D010649:Phenylalanine / Q000494:pharmacology; D012333:RNA, Messenger / Q000032:analysis; D012680:Sensitivity and Specificity; D017386:Sequence Homology, Amino Acid; D012867:Skin / Q000196:embryology* / Q000201:enzymology; D013773:Tetramisole / Q000494:pharmacology
D012333:RNA, Messenger; D006709:Homoarginine; D007978:Levamisole; D010649:Phenylalanine; D004247:DNA; D013773:Tetramisole; D000469:Alkaline Phosphatase