title: Characterization and chromosomal localization of a new protein disulfide isomerase, PDIp, highly expressed in human pancreas.
abstract: Protein disulfide isomerase (PDI) catalyzes protein folding and thiol-disulfide interchange reactions. The enzyme is localized in the lumen of endoplasmic reticulum (ER) and is abundant in secretory cells of various tissues. In this study we describe the isolation and characterization from human pancreas of a new protein, PDIp, that is structurally and functionally related to PDIs. PDIp cDNA is 1,659 bp in length and predicts a protein with an open reading frame of 511 amino acids. PDIp amino acid sequence shows 46% identity and 66% similarity to that of human PDI. PDIp possesses two thioredoxin-like active sites (WCGHCQ and WCTHCK) and an endoplasmic reticulum retention signal sequence, KEEL, at the carboxyl terminus. Northern analysis of normal human tissues and various human tumor cell lines revealed PDIp mRNA (2.0 kb) expression only in the normal pancreas. Recombinant PDIp protein catalyzed reductive cleavage of insulin and renaturation of reduced RNaseA. Somatic cell genetics and fluorescence in situ hybridization localized the PDIp gene to the short arm of human chromosome 16. It is concluded that PDIp is a new member of the PDI family and is highly expressed in human pancreas.
authors.affiliation: Laboratory of Oral Medicine, National Institute of Dental Research, National Institutes of Health, Bethesda, MD 20892-4322, USA.
authors.forename: M G
authors.initials: MG
authors.lastname: Desilva
authors.forename: J
authors.initials: J
authors.lastname: Lu
authors.forename: G
authors.initials: G
authors.lastname: Donadel
authors.forename: W S
authors.initials: WS
authors.lastname: Modi
authors.forename: H
authors.initials: H
authors.lastname: Xie
authors.forename: A L
authors.initials: AL
authors.lastname: Notkins
authors.forename: M S
authors.initials: MS
authors.lastname: Lan
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D002885:Chromosomes, Human, Pair 16; D003001:Cloning, Molecular; D006801:Humans; D007535:Isomerases / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010179:Pancreas / Q000201:enzymology*; D010802:Phylogeny; D019704:Protein Disulfide-Isomerases; D016415:Sequence Alignment; D017421:Sequence Analysis
chemical_list: D007535:Isomerases; D019704:Protein Disulfide-Isomerases