title: Construction of a YAC contig and an STS map spanning 3.6 megabase pairs in Xp22.1.
abstract: We have constructed a 3.6 Mb sequence tagged sites (STS)-based yeast artificial chromosome (YAC) contig, consisting of 58 individual YAC clones, spanning the region PDHA1 and DXS451 on Xp22.1. In addition to establishing the order of PDHA1, ISPK-1, DXS2504, DXS1528 and the 13 known polymorphic loci as Xpter-PDHA1-DXS443-DXS3424-ISPK-1-DXS12 29-DXS2504-DXS1528-DXS365-DXS7101- DXS1683-DXS1052-DXS274-DXS92-DXS1226-DX S41-DXS989-DXS451-Xcen, we have also developed 35 novel STSs from YAC end clones. These results provide a high density of STS markers (approximately 1 per 70 kb). Furthermore, a detailed long-range restriction map of the contig has been constructed with rare-cutter enzymes and this has refined and verified the physical distances between markers inferred from YAC sizes and their STS content. The integration of the physical mapping data with previous genetic mapping data and the use of STSs and non-chimeric YAC clones reported here should facilitate the construction of a transcript map of this region and the positional cloning of disease genes in this portion of Xp22.1.
authors.affiliation: MRC Molecular Endocrinology Group, Royal Postgraduate Medical School, Hammersmith Hospital, London, UK.
authors.forename: D
authors.initials: D
authors.lastname: Trump
authors.forename: G
authors.initials: G
authors.lastname: Pilia
authors.forename: P H
authors.initials: PH
authors.lastname: Dixon
authors.forename: C
authors.initials: C
authors.lastname: Wooding
authors.forename: R
authors.initials: R
authors.lastname: Thakrar
authors.forename: S E
authors.initials: SE
authors.lastname: Leigh
authors.forename: R
authors.initials: R
authors.lastname: Nagaraja
authors.forename: M P
authors.initials: MP
authors.lastname: Whyte
authors.forename: D
authors.initials: D
authors.lastname: Schlessinger
authors.forename: R V
authors.initials: RV
authors.lastname: Thakker
mesh_terms: D001482:Base Composition; D001483:Base Sequence; D002874:Chromosome Mapping; D018244:Chromosomes, Artificial, Yeast; D017931:DNA Primers; D005819:Genetic Markers; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping; D016324:Sequence Tagged Sites; D014960:X Chromosome
chemical_list: D017931:DNA Primers; D005819:Genetic Markers