Separation of tryptophan and related indoles by micellar electrokinetic chromatography with KrF laser-induced fluorescence detection.
Micellar electrokinetic chromatography (MEKC) was applied for the separation of tryptophan and related indoles. Using a 5 mM sodium borate buffer (pH 9.2) containing 50 mM sodium dodecyl sulfate and 5% acetonitrile, eleven indoles were baseline separated in under 17 min. Most of the indoles were detected at the nM level by native fluorescence using KrF laser-induced fluorescence (LIF), which was approximately 100 times more sensitive than UV absorption detection at 200 nm. Preliminary results show that the MEKC-LIF with direct sample injection is a feasible method for assessing indole profiles in diluted urine and serum.
Chan
K C
KC
SAIC Frederick, National Cancer Institute, Frederick Cancer Research and Development Center, MD 21702, USA.
Muschik
G M
GM
Issaq
H J
HJ
D019075:Electrophoresis, Capillary / Q000379:methods*; D005459:Fluorides; D007211:Indoles / Q000032:analysis*; D007726:Krypton; D007834:Lasers; D008823:Micelles; D015394:Molecular Structure; D012967:Sodium Dodecyl Sulfate; D013050:Spectrometry, Fluorescence; D014364:Tryptophan / Q000032:analysis*
D007211:Indoles; D008823:Micelles; C047729:krypton fluoride; D012967:Sodium Dodecyl Sulfate; D007726:Krypton; D014364:Tryptophan; D005459:Fluorides