Abolition of suppressive effect of acute myeloid leukemia cells on normal granulocyte-macrophage colony formation induced by interleukin-5 associated with eosinophilic cell induction.
The effects of recombinant interleukin-5 (rIL-5) on leukemic blasts obtained from 22 acute myeloid leukemia (AML) patients were investigated. Recombinant IL-5 stimulated leukemic colony formation in the leukemic blasts of 50% of the patients (11/22), and in 7 of these 11 cell cultures leukemic eosinophilic colonies were induced. Eosinophilic cell induction was associated with cellular proliferation, that is, colony or cluster formation. Leukemic blasts cultured with rIL-5 and forming eosinophilic colonies no longer suppressed normal granulocyte-macrophage colony formation, suggesting that functional differentiation of these leukemic blasts took place. Induction of this type of functional differentiation of leukemic blasts may be of clinical value in promoting normal hematopoiesis.
Motoji
T
T
Department of Medicine, Tokyo Women's Medical college, Japan.
Okada
M
M
Takanashi
M
M
Oshimi
K
K
Mizoguchi
H
H
D000208:Acute Disease; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002450:Cell Communication / Q000187:drug effects*; D002454:Cell Differentiation / Q000187:drug effects; D004804:Eosinophils / Q000166:cytology*; D005260:Female; D006098:Granulocytes / Q000166:cytology* / Q000187:drug effects; D006412:Hematopoietic Stem Cells / Q000187:drug effects*; D006801:Humans; D015848:Interleukin-5 / Q000494:pharmacology*; D007951:Leukemia, Myeloid / Q000188:drug therapy / Q000473:pathology*; D008264:Macrophages / Q000166:cytology* / Q000187:drug effects; D008297:Male; D008875:Middle Aged; D011994:Recombinant Proteins / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D015848:Interleukin-5; D011994:Recombinant Proteins