HI-6 therapy and the acute phase response in the rat.
The propensity of therapeutic doses of HI-6 (50 mg/kg) in combination with atropine sulphate (17 mg/kg), antidotes used to treat organophosphate poisoning, to induce the acute phase response (APR) in the laboratory rat was examined. A single intraperitoneal injection of HI-6, either alone or with atropine, caused a rapid doubling of the plasma corticosterone concentration. However, this increase was short-lived in comparison with corticosterone kinetics during the typical, turpentine-induced APR. The elevated glucocorticosteroid concentration did not affect acute phase protein (APP) gene transcription or mRNA and protein synthesis in the livers of oxime/atropine-treated rats. On the basis of these findings, we concluded that the administered doses of HI-6 and atropine did not induce the generalised, non-specific APR.
Ivanović-Matić
S
S
Laboratory for Molecular Biology, Institute for Biological Research 29, Belgrade, Yugoslavia.
Poznanović
G
G
D000209:Acute-Phase Proteins / Q000096:biosynthesis* / Q000235:genetics; D000210:Acute-Phase Reaction / Q000139:chemically induced*; D000818:Animals; D000931:Antidotes / Q000008:administration & dosage / Q000627:therapeutic use / Q000633:toxicity*; D001285:Atropine / Q000008:administration & dosage / Q000627:therapeutic use / Q000633:toxicity; D001798:Blood Proteins / Q000096:biosynthesis; D015152:Blotting, Northern; D003345:Corticosterone / Q000097:blood; D007122:Immunoelectrophoresis; D007274:Injections, Intraperitoneal; D008099:Liver / Q000378:metabolism; D008297:Male; D010091:Oximes; D011726:Pyridinium Compounds / Q000008:administration & dosage / Q000627:therapeutic use / Q000633:toxicity*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D012999:Soman / Q000506:poisoning; D014425:Turpentine / Q000633:toxicity
D000209:Acute-Phase Proteins; D000931:Antidotes; D001798:Blood Proteins; D010091:Oximes; D011726:Pyridinium Compounds; D012333:RNA, Messenger; D001285:Atropine; D012999:Soman; C022870:asoxime chloride; D003345:Corticosterone; D014425:Turpentine