Human gastric inhibitory polypeptide receptor: cloning of the gene (GIPR) and cDNA.
Gastric inhibitory polypeptide (GIP), which is released from the gastrointestinal tract, stimulates insulin secretion from pancreatic beta cells and plays a crucial role in the regulation of insulin secretion during the postprandial phase. We have isolated the human gene (GIPR) and cDNA encoding the GIP receptor by a combination of the conventional screening and polymerase chain reaction procedures. Human GIP receptor cDNA encodes a protein of 466 amino acids that is 81.5 and 81.2% identical to the previously cloned hamster and rat GIP receptor, respectively. Hydropathic analysis shows the presence of a signal peptide and seven potential transmembrane domains, a feature characteristic of the VIP/glucagon/secretin receptor family of G protein-coupled receptors. The human GIPR gene is about 13.8 kb long, consists of 14 exons, and carries 17 Alu repeats.
Yamada
Y
Y
Department of Metabolism and Clinical Nutrition, Kyoto University Faculty of Medicine, Japan.
Hayami
T
T
Nakamura
K
K
Kaisaki
P J
PJ
Someya
Y
Y
Wang
C Z
CZ
Seino
S
S
Seino
Y
Y
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D006224:Cricetinae; D018076:DNA, Complementary; D005091:Exons; D005749:Gastric Inhibitory Polypeptide / Q000494:pharmacology / Q000502:physiology; D006801:Humans; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D007438:Introns; D007515:Islets of Langerhans / Q000378:metabolism; D008969:Molecular Sequence Data; D051381:Rats; D011964:Receptors, Gastrointestinal Hormone / Q000096:biosynthesis* / Q000235:genetics*; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D007328:Insulin; D011964:Receptors, Gastrointestinal Hormone; D005749:Gastric Inhibitory Polypeptide; C041086:gastric inhibitory polypeptide receptor