Interleukin 12 potentiates the curative effect of a vaccine based on interleukin 2-transduced tumor cells.
The purpose of these studies was to determine whether systemic administration of recombinant interleukin 12 (rIL-12) is able to potentiate an initial, but insufficient T-cell antitumor response. Mice challenged with carcinoma cells engineered to release interleukin 2 (IL-2) and displaying such a response received single or multiple i.p. injections of rIL-12. This combination of systemic rIL-12 and local IL-2 increased the percentage of mice that rejected two different IL-2 gene-transduced tumors. In another set of experiments more closely resembling a clinical situation, IL-2 gene-transduced tumors were used as vaccines in an attempt to cure mice bearing wild-type parental tumors. The combination of these vaccines with systemic rIL-12 cured mice more effectively than rIL-12 and IL-2 gene-transduced tumor vaccines alone.
Vagliani
M
M
Istituto Nazionale Tumori, Milan, Italy.
Rodolfo
M
M
Cavallo
F
F
Parenza
M
M
Melani
C
C
Parmiani
G
G
Forni
G
G
Colombo
M P
MP
D000230:Adenocarcinoma / Q000276:immunology* / Q000378:metabolism; D000818:Animals; D002455:Cell Division / Q000502:physiology; D017024:Chemotherapy, Adjuvant; D003110:Colonic Neoplasms / Q000276:immunology* / Q000378:metabolism; D003131:Combined Modality Therapy; D018076:DNA, Complementary / Q000235:genetics; D004357:Drug Synergism; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D006801:Humans; D016219:Immunotherapy, Adoptive; D018664:Interleukin-12 / Q000494:pharmacology*; D007376:Interleukin-2 / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D007694:Killer Cells, Natural / Q000276:immunology; D016246:Lymphocytes, Tumor-Infiltrating / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D013601:T-Lymphocytes / Q000276:immunology; D014161:Transduction, Genetic; D014612:Vaccines / Q000276:immunology / Q000494:pharmacology*
D018076:DNA, Complementary; D007376:Interleukin-2; D014612:Vaccines; D018664:Interleukin-12; D016178:Granulocyte-Macrophage Colony-Stimulating Factor