[Rapid assay of plasma acetate by gas chromatography: evaluation and comparison with two other methods].
Human plasma acetate is derived from colonic fermentation of fiber and endogenous metabolism of dextrose and fatty acids. Acetate may have regulatory functions in hepatic carbohydrate metabolism. Intake of dietary fiber is associated with several beneficial effects on carbohydrates and lipids metabolisms. To study theses effects a valid and automated method for routine analysis of acetate in plasma is necessary. After oral administration of lactulose to healthy human volunteers, the concentration of plasma acetate was measured by head space gas chromatography (HS-GC), vacuum distillation gas chromatography (VD-GC) and enzymatic spectrometric method (ES). The method HS-GC was linear to 0.5 mmol.l-1 (n = 5, r = 0.998), the detection limit is 0.005 mmol.l-1. Within-day variation (CV) was 3.60% and day-to-day variation was 4.5% (0.1 mmol.l-1). The coefficients of correlation between CG-ET/CG-DsV and CG-ET/E-M are 0.903 (p = 0.0001) and 0.54 (p = 0.006) respectively, the mean square errors are respectively 0.118 and 0.138 mmol.l-1. The variation curves of plasma acetate measured by GC versus time show peak concentration of 0.323 to 0.380 mmol.l-1 at 120 min.
Boussairi
A
A
Laboratoire de biochimie-toxicologie, Paris, France.
Alamowitch
C
C
Hoebler
C
C
Champ
M
M
Bornet
F
F
Guyon
F
F
D000085:Acetates / Q000097:blood*; D002849:Chromatography, Gas / Q000379:methods*; D006801:Humans; D015203:Reproducibility of Results
D000085:Acetates
Dosage rapide de l'acétate plasmatique par chromatographie en phase gazeuse--technique espace de tête: évaluation et comparaison avec deux autres méthodes.