Cloning, expression, and mapping of UBE2I, a novel gene encoding a human homologue of yeast ubiquitin-conjugating enzymes which are critical for regulating the cell cycle.
From a human fetal-brain cDNA library we isolated a novel gene sharing significant homology with two yeast genes, UBC9 and hus5, which encode ubiquitin-conjugating enzyme 9 (UBC9). In yeast this protein is critical for normal mitosis, and seems to be closely involved in progression of G2 to M phase of the cell cycle. The human UBC9 (h-UBC9) cDNA, (gene symbol UBE2I), contained an open reading frame of 474 nucleotides encoding 158 amino acids. Its predicted peptide showed respectively 56% and 66% identity (75% and 82% similarity) with the products of UBC9 and hus5. Northern-blot analysis revealed expression of three transcripts, 6.4 kb, 3.3 kb, and 1.35 kb, in all human tissues examined. This gene, UBE2I, was mapped to chromosome band 16p13.3 by FISH.
Watanabe
T K
TK
Otsuka GEN Research Institute, University of Tokyo, Japan.
Fujiwara
T
T
Kawai
A
A
Shimizu
F
F
Takami
S
S
Hirano
H
H
Okuno
S
S
Ozaki
K
K
Takeda
S
S
Shimada
Y
Y
Nagata
M
M
Takaichi
A
A
Takahashi
E
E
Nakamura
Y
Y
Shin
S
S
D000595:Amino Acid Sequence; D001483:Base Sequence; D002453:Cell Cycle; D002874:Chromosome Mapping; D002885:Chromosomes, Human, Pair 16; D003001:Cloning, Molecular; D017931:DNA Primers / Q000737:chemistry; D015870:Gene Expression; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008025:Ligases / Q000235:genetics*; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D014452:Ubiquitins
D017931:DNA Primers; D014452:Ubiquitins; D008025:Ligases