Novel GUS expression patterns following transposition of an enhancer trap Ds element in Arabidopsis.
Enhancer trap derivatives of the maize Dissociation (Ds) transposon were introduced into Arabidopsis thaliana. The enhancer trap Ds was so designed that upon transposition to sites containing regulatory sequences in adjacent genomic DNA, transcription of a Ds-borne beta-glucuronidase (GUS) gene would be activated. Sixty percent of all transposition events were associated with GUS expression patterns including one linked to a mutant phenotype. Patterns of GUS expression were found in various organs and were stably inheritable in the F4 and F5 progenies. These results demonstrate the potential value of the technique as a means for detection of developmentally regulated genes and analysis of their function. The enhancer trap construct used in our experiments, as well as the seeds of primary transformants are publicly available.
Klimyuk
V I
VI
Sainsbury Laboratory, John Innes Institute, Norwich, United Kingdom.
Nussaume
L
L
Harrison
K
K
Jones
J D
JD
D017360:Arabidopsis / Q000235:genetics*; D001483:Base Sequence; D015139:Blotting, Southern; D004251:DNA Transposable Elements / Q000235:genetics*; D004742:Enhancer Elements, Genetic / Q000235:genetics*; D018506:Gene Expression Regulation, Plant / Q000235:genetics; D017930:Genes, Reporter / Q000235:genetics; D005966:Glucuronidase / Q000235:genetics* / Q000378:metabolism; D006651:Histocytochemistry; D008858:Microscopy, Phase-Contrast; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D010641:Phenotype; D016133:Polymerase Chain Reaction; D016385:TATA Box / Q000235:genetics; D015533:Transcriptional Activation; D014170:Transformation, Genetic / Q000235:genetics
D004251:DNA Transposable Elements; D005966:Glucuronidase