title: Translation of RNAIII, the Staphylococcus aureus agr regulatory RNA molecule, can be activated by a 3'-end deletion.
abstract: RNAIII, an RNA molecule shown to encode delta-hemolysin and independently to regulate toxin synthesis in Staphylococcus aureus, is transcribed at the mid-exponential phase of growth, while its target genes are activated 2 h later, at the post-exponential phase of growth. We show here that the translation of RNAIII to the 26-amino acid peptide delta-hemolysin is delayed by 1 h, and that this delay is abolished when the 3'-end of this molecule is deleted. We suggest that structural changes of RNAIII to a translatable form of the molecule precede its regulation of target gene expression.
authors.affiliation: California Regional Primate Research Center, University of California, Davis 95616, USA.
authors.forename: N
authors.initials: N
authors.lastname: Balaban
authors.forename: R P
authors.initials: RP
authors.lastname: Novick
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D001427:Bacterial Toxins / Q000235:genetics; D001483:Base Sequence; D002455:Cell Division / Q000235:genetics; D017353:Gene Deletion; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics; D006460:Hemolysin Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D009498:Neurotoxins / Q000235:genetics; D009690:Nucleic Acid Conformation; D014176:Protein Biosynthesis; D016372:RNA, Antisense / Q000235:genetics*; D012329:RNA, Bacterial / Q000235:genetics*; D013211:Staphylococcus aureus / Q000166:cytology / Q000235:genetics*; D014158:Transcription, Genetic / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D001427:Bacterial Toxins; D006460:Hemolysin Proteins; D009498:Neurotoxins; D016372:RNA, Antisense; D012329:RNA, Bacterial; C096035:RNAIII, Staphylococcus aureus; C021091:staphylococcal alpha-toxin; C025994:delta hemolysin protein, Staphylococcus aureus