Inhibition of microsomal chemiluminescence by cytosolic fractions containing fatty acid binding protein.
Studies were carried out to determine the relationship between Fe(++)-ascorbate initiated chemiluminescence and lipid peroxidation in rat liver, lung, kidney and brain microsomes. In order to follow the time course of membrane lipid peroxidation, we measured simultaneously physical and biochemical changes. Thus we determined the fatty acid composition of microsomal membranes from those tissues after peroxidation with and without ascorbic acid. Fractionation of cytosolic proteins with ammonium sulfate 70% saturation yielded a soluble fraction (enriched in fatty acid binding protein) that inhibited ascorbate-Fe++ dependent lipid peroxidation. The inhibitory effect was concentration dependent and was not abolished by heating of the soluble fraction during 5 min at 100 degrees C. Preparations from kidney, lung, heart and brain showed similar effect on lipid peroxidation of liver microsomal membranes. It is especulated that retinyl esters bound to fatty acid binding protein may act as antioxidants against lipid peroxidation.
Catala
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Cátedra de Bioquímica, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, Argentina.
Cerruti
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Arcemis
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D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology; D001921:Brain / Q000648:ultrastructure; D002352:Carrier Proteins / Q000302:isolation & purification / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism*; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D005227:Fatty Acids / Q000032:analysis; D005296:Ferrous Compounds / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007668:Kidney / Q000648:ultrastructure; D015227:Lipid Peroxidation / Q000187:drug effects; D008163:Luminescent Measurements; D008168:Lung / Q000648:ultrastructure; D008861:Microsomes / Q000378:metabolism*; D008862:Microsomes, Liver / Q000378:metabolism; D018996:Myelin P2 Protein / Q000302:isolation & purification / Q000378:metabolism*; D009206:Myocardium / Q000648:ultrastructure; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D051381:Rats; D017208:Rats, Wistar
D002352:Carrier Proteins; C490174:Fabp7 protein, rat; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D005227:Fatty Acids; D005296:Ferrous Compounds; D018996:Myelin P2 Protein; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D001205:Ascorbic Acid