title: Long-term effects of calcium availability on prolactin and protein synthesis in human decidual cells.
abstract: Ever since decidual cells were recognized as the source of decidual prolactin (dPRL), very few reports have dealt with the role of calcium (Ca2+) on dPRL synthesis and release. In a recent work, we described the presence of T-type Ca2+ channels in these cells, giving Ca(2+)-dependent action potentials. However, we failed to demonstrate any action of decidual cell Ca2+ modulation on acute dPRL release, but observed only long-term effects. We have now investigated these effects on decidual protein and dPRL synthesis after 24 h treatments. When Ca2+ channel blockers or EGTA (2 mM) were added to the culture medium, dPRL release and [3H] leucine incorporation into proteins decreased. Increasing external Ca2+ up to 2 mM instead of 0.8 mM or changing the external K+ concentration (30 mM instead of 5.6) had no consequence on dPRL release, whereas 2 mM of Ca2+ enhanced total protein synthesis. No toxicity was noted with these treatments. Finally a possible effect of Ca2+ modulation on dPRL synthesis was studied using [35S] methionine. The specific activity of [35S] methionine on dPRL was similar in control and treated cells (EGTA, 2 mM Ca2+, cobalt). These results support the idea that Ca2+ controls dPRL synthesis in decidual cells, acting only on general protein synthesis processes.
authors.affiliation: Laboratoire de Neurophysiologie CNRS URA 1200, Université de Bordeaux 2, France.
authors.forename: B
authors.initials: B
authors.lastname: Couderc
authors.forename: L
authors.initials: L
authors.lastname: Dufy-Barbe
authors.forename: P
authors.initials: P
authors.lastname: Sartor
mesh_terms: D000704:Analysis of Variance; D002118:Calcium / Q000502:physiology*; D002478:Cells, Cultured; D003656:Decidua / Q000166:cytology / Q000378:metabolism*; D005069:Evaluation Studies as Topic; D005260:Female; D006801:Humans; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000096:biosynthesis*; D011388:Prolactin / Q000096:biosynthesis* / Q000378:metabolism; D012016:Reference Values; D013997:Time Factors
chemical_list: D011257:Pregnancy Proteins; D011388:Prolactin; D002118:Calcium