Fertilization and pregnancies following intracytoplasmic injection of testicular spermatozoa.
OBJECTIVE
Intracytoplasmic injection (ICSI) with testicular sperm was performed in 16 couples. All men had ductal obstruction and failed previous attempts of epididymal sperm microaspiration.


METHODS
Testis tissue was obtained by excisional biopsies and incubated in HEPES buffered EBSS medium over 24 h at 37 degrees C. Motile sperm (Grade 1 to 2) were recovered in 13 patients and fertilized a total of 62 oozytes. Four pregnancies were achieved.


RESULTS
One healthy boy and two girls (twin pregnancy) were born.


CONCLUSIONS
The ongoing pregnancies revealed no fetal abnormalities on ultrasound scanning.
Obruca
A
A
Institute for Sterility Treatment, Wien, Austria.
Mock
K
K
Feichtinger
W
W
Lunglmayr
G
G
D000328:Adult; D001706:Biopsy; D005260:Female; D005306:Fertilization / Q000502:physiology*; D005307:Fertilization in Vitro / Q000379:methods / Q000592:standards*; D006801:Humans; D007248:Infertility, Male / Q000503:physiopathology / Q000628:therapy; D008297:Male; D008845:Microinjections; D008846:Micromanipulation; D008875:Middle Aged; D009865:Oocytes / Q000166:cytology / Q000502:physiology; D011247:Pregnancy / Q000502:physiology*; D011256:Pregnancy Outcome; D018873:Pregnancy Rate; D013081:Sperm Motility / Q000502:physiology; D013084:Sperm-Ovum Interactions / Q000502:physiology*; D013094:Spermatozoa / Q000166:cytology / Q000502:physiology*; D013737:Testis / Q000166:cytology / Q000502:physiology*