Genes leave their characteristic marks on the melting profile of plasmid DNAs.
High resolution melting profiles of four linearised plasmids (pUC9, pGV403, pHP2, and pBR322) were recorded by means of UV absorbance vs. temperature scanning. The set of transitions obtained for each plasmid are compared to each other and to the transitions obtained for their excised particular antibiotica resistance gene. It can be shown that each gene leaves a characteristic mark on the melting profile of its parental plasmid.
Zubrzycki
I Z
IZ
Klump
H H
HH
D000668:Ampicillin Resistance / Q000235:genetics; D002702:Chloramphenicol Resistance / Q000235:genetics; D005798:Genes, Bacterial; D006358:Hot Temperature; D009691:Nucleic Acid Denaturation; D010957:Plasmids / Q000737:chemistry* / Q000235:genetics*; D013753:Tetracycline Resistance / Q000235:genetics; D013816:Thermodynamics