title: Isolation and partial characterization of yeast mannan hydrolysing enzymes from bacterial isolates.
abstract: A range of aerobic, mesophilic bacterial strains capable of producing extracellular alpha-mannanase and alpha-mannosidase enzymes were isolated from various natural habitats using yeast cell wall material as the selective medium. Five of them were capable of producing extracellular alpha-mannosidase and alpha-mannanase on yeast mannan as the sole carbon substrate, and they were tentatively identified to the genus level as Arthrobacter, Streptomyces, Pseudomonas, Bacillus and Acinetobacter. Among these strains, Arthrobacter M-02 showed the highest biosynthetic activity of the enzymes. The crude enzyme preparations from the strains were active at pH 4 to 9, reaching a maximum activity between 5.5 and 6.8. The crude Arthrobacter M-02 alpha-mannosidase was more thermostable than that of the other strains, but the alpha-mannanase of the strain was thermolabile.
authors.affiliation: Department of Bioscience and Biotechnology, University of Strathclyde, Glasgow, Scotland, Great Britain.
authors.forename: M A
authors.initials: MA
authors.lastname: Malek
authors.forename: D R
authors.initials: DR
authors.lastname: Berry
mesh_terms: D000150:Acinetobacter / Q000201:enzymology; D001173:Arthrobacter / Q000201:enzymology; D001407:Bacillus / Q000201:enzymology; D001419:Bacteria / Q000201:enzymology*; D003470:Culture Media; D006863:Hydrogen-Ion Concentration; D006867:Hydrolases / Q000302:isolation & purification / Q000378:metabolism*; D008351:Mannans / Q000378:metabolism*; D008361:Mannosidases / Q000302:isolation & purification / Q000378:metabolism*; D011549:Pseudomonas / Q000201:enzymology; D013302:Streptomyces / Q000201:enzymology; D013379:Substrate Specificity; D015003:Yeasts / Q000378:metabolism*; D043323:alpha-Mannosidase
chemical_list: D003470:Culture Media; D008351:Mannans; D006867:Hydrolases; D008361:Mannosidases; D043323:alpha-Mannosidase; C048377:mannan 1,2-(1,3)-alpha mannosidase