Nuclear localization of procathepsin L/MEP in ras-transformed mouse fibroblasts.
It has been well-documented that secretion of procathepsin L is enhanced in ras-oncogene-transformed cells. In the present study, intracellular localization of cathepsin L was investigated by cell fractionation using Nonidet P-40 followed by immunoblot analysis. The results showed that a significant amount of procathepsin L was detectable in the nuclear fraction of Ha-ras, Ki-ras- and erbB2-transformed NIH3T3 mouse fibroblasts while procathepsin L was detected only in the cytoplasmic fraction of NIH3T3 cells and v-mos-transformed cells. These results suggest that the processing and translocation of cathepsin L are seriously impeded in ras- and erbB2-transformed cells.
Hiwasa
T
T
Division of Biochemistry, Chiba Cancer Center Research Institute, Japan.
Sakiyama
S
S
D016475:3T3 Cells / Q000187:drug effects / Q000201:enzymology*; D000595:Amino Acid Sequence; D000818:Animals; D056668:Cathepsin L; D002403:Cathepsins / Q000378:metabolism*; D002467:Cell Nucleus / Q000201:enzymology; D002471:Cell Transformation, Neoplastic; D003546:Cysteine Endopeptidases; D003593:Cytoplasm / Q000201:enzymology; D003907:Dexamethasone / Q000494:pharmacology; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D010450:Endopeptidases; D004792:Enzyme Precursors / Q000378:metabolism*; D015870:Gene Expression / Q000187:drug effects; D018734:Genes, erbB-2; D011905:Genes, ras; D015151:Immunoblotting; D051379:Mice; D008969:Molecular Sequence Data
D004792:Enzyme Precursors; D003907:Dexamethasone; D002403:Cathepsins; D010450:Endopeptidases; D003546:Cysteine Endopeptidases; C063905:procathepsin L; D056668:Cathepsin L; C534278:Ctsl protein, mouse; D004121:Dimethyl Sulfoxide