Effect of cations on the elution of pectic polysaccharides from anion-exchange resins.
Conditions for quantitatively eluting six plant pectic polysaccharides from diethylaminoethyl (DEAE)-columns were established. Cations, surprisingly, greatly affected the elution of pectic polysaccharides from these anion-exchange columns. Quantitative elution of apple pectic acid was achieved when columns of DEAE-Sephadex A-25 and DEAE-Trisacryl Plus-M were developed with 0.5 M NH4Cl, 0.5 M LiCl, or 0.5 M CsCl in buffer. In contrast, up to 1 M NaCl and KCl in buffer only eluted 6.3 to 54% of the apple pectic acid from the two types of columns. In each case the retained apple pectic acid was eluted basically quantitatively by 0.5 M NH4Cl in buffer. The elution of a pectic polysaccharide fraction from Apium graveolens (celery), commercial citrus polygalacturonic acid, and two polysaccharides isolated from purified cell walls of Lemna minor (duckweed) from columns of DEAE-Trisacryl was incomplete when the columns were developed wit 0.5 M NaCl or 0.5 M KCl in buffer, ranging from 0 to 89%; however, again, the retained portion of each sample was eluted quantitatively or almost so from the columns with 0.5 M NH4Cl in buffer. One pectic polysaccharide, commercial citrus pectin, was eluted equally well and almost quantitatively by 0.5 M NaCl, KCl and NH4Cl in buffer. When a pectic polysaccharide fraction from purified cell walls of L. minor was applied to a preparative column of DEAE-Trisacryl Plus-M, 99% of the sample was eluted by a gradient of 0 to 0.5 M NH4Cl in buffer.(ABSTRACT TRUNCATED AT 250 WORDS)
Cheng
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L
Department of Biochemistry, Michigan State University, East Lansing 48824-1319, USA.
Kindel
P K
PK
D000838:Anions; D002412:Cations; D002852:Chromatography, Ion Exchange / Q000379:methods*; D010944:Plants / Q000737:chemistry; D011134:Polysaccharides / Q000302:isolation & purification*; D012116:Resins, Plant / Q000737:chemistry*
D000838:Anions; D002412:Cations; D011134:Polysaccharides; D012116:Resins, Plant