Specificities of rabbit antisera to multiple antigen (MAP) peptides.
Two multiple antigen peptides consisting of 6 and 7 aminoa cid residues, respectively, plus a 12-residue fragment, used as a control, all linked to a polylysine core, were used as immunogens in rabbits in order to obtain an immune response. Rabbit antisera against such polymers were then tested in ELISA against a panel of antigens in order to analyze the specificities of the resulting antibodies. The responses were different for all three immunogens, being partially or totally directed, for two of the three compounds, including the 12-residue control MAP peptide, against the polylysyl core, which is considered as non immunogenic. The third MAP polymer was practically unable to elicit an immune response.
Chersi
A
A
Regina Elena Institute for Cancer Research, CRS, Laboratory of Biochemistry, Roma, Italy.
di Modugno
F
F
Falasca
G
G
D000595:Amino Acid Sequence; D000818:Animals; D000917:Antibody Formation; D000918:Antibody Specificity; D000941:Antigens / Q000737:chemistry / Q000276:immunology*; D001666:Binding Sites, Antibody; D004797:Enzyme-Linked Immunosorbent Assay; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000737:chemistry / Q000276:immunology*; D010455:Peptides / Q000737:chemistry / Q000276:immunology*; D011817:Rabbits / Q000276:immunology; D013329:Structure-Activity Relationship
D000941:Antigens; D009842:Oligopeptides; D010455:Peptides