title: Transcription-induced deletions in Escherichia coli plasmids.
abstract: Characterization of functions that render DNA susceptible to rearrangement is important for a better understanding of genome instability. In a previous work, we showed that sequences located downstream of a strong promoter are particularly prone to deletion. In this paper, the parameters that influence transcription-induced deletions were studied. pBR322 derivatives carrying the M13 (+) replication origin and a PTac-dependent transcription region were used. Deletion formation was analysed in the presence of the replication protein of M13, which introduces a nick at the phage replication origin, and in a rep- strain to avoid M13-driven replication. Our study showed that: (i) 4 h after induction of transcription, a few per cent of the plasmids have experienced a deletion; (ii) these deletions result in joining of the M13 replication origin to a nucleotide located in or downstream of the transcribed region; (iii) deletion formation strongly depends on the orientation of transcription, on promoter strength and transcript length, but is independent of translation; (iv) formation of transcription-induced supercoiling domains does not induce deletion formation. We propose that deletions in the transcribed region result from collisions between converging replication and transcription machineries.
authors.affiliation: Laboratoire de Génétique Microbienne, INRA Domaine de Vilvert, Jouy en Josas, France.
authors.forename: D
authors.initials: D
authors.lastname: Vilette
authors.forename: S D
authors.initials: SD
authors.lastname: Ehrlich
authors.forename: B
authors.initials: B
authors.lastname: Michel
mesh_terms: D001483:Base Sequence; D004261:DNA Replication / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004278:DNA, Superhelical / Q000737:chemistry / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D007763:Lac Operon; D008969:Molecular Sequence Data; D010957:Plasmids / Q000737:chemistry / Q000235:genetics*; D017384:Sequence Deletion; D014158:Transcription, Genetic
chemical_list: D004269:DNA, Bacterial; D004278:DNA, Superhelical