title: 5,7-Dihydroxytryptamine uptake discriminates living serotonergic cells from dopaminergic cells in rat midbrain culture.
abstract: Dissociated cells from embryonic rat midbrain develop in dissociated culture into glutamatergic, GABAergic and aminergic cells. The autofluorescent serotonin analogue, 5,7-dihydroxytryptamine (5,7-DHT), is taken up by a small population of cells that is immunoreactive to 5-hydroxytryptamine. Tyrosine hydroxylase-immunoreactive cells do not accumulate 5,7-DHT. 5,7-DHT uptake, therefore, is well suited for the identification of living serotonergic cells and their discrimination from dopaminergic cells.
authors.affiliation: I. Physiologisches Institut, Universität Heidelberg, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Rohrbacher
authors.forename: K
authors.initials: K
authors.lastname: Krieglstein
authors.forename: S
authors.initials: S
authors.lastname: Honerkamp
authors.forename: A
authors.initials: A
authors.lastname: Lewen
authors.forename: U
authors.initials: U
authors.lastname: Misgeld
mesh_terms: D015116:5,7-Dihydroxytryptamine / Q000493:pharmacokinetics*; D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D004298:Dopamine / Q000378:metabolism*; D007150:Immunohistochemistry; D008636:Mesencephalon / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism; D051381:Rats; D012701:Serotonin / Q000378:metabolism*
chemical_list: D015116:5,7-Dihydroxytryptamine; D012701:Serotonin; D004298:Dopamine