Effect of CD80 and CD86 on T cell cytokine production.
Conjugation of the T cell receptor (TCR) with antigen/MHC proteins must be accompanied by conjugation of T cell counterreceptors (CD28 or CTLA-4) with costimulatory molecules CD80 or CD86 (B7-1 or B7-2) on antigen presenting cells (APC) to avert T cell anergy, and to provide essential signals for T cell activation and cytokine production. However, T cells and APC express changing patterns of counterreceptors and costimulatory molecules during the immune response. To determine the involvement of CD80 and CD86 in costimulation of T cell cytokine production, T cells were incubated with peritoneal exudate macrophages, which express CD80 and CD86, and stimulated in vitro for 48 or 72 hrs with anti-CD3 in the presence or absence of blocking antibody to CD80 or CD86. Alternatively, enriched anti-CD3 stimulated T cells were costimulated with antibody to CD28 and CTLA-4. Production of T cell IL-2, IL-4, and IL-5 was depressed in the presence of anti-CD86 but not anti-CD80. Production of IFN-gamma was significantly blocked by either anti-CD80 and anti-CD86. Anti-CD28 was a potent costimulator of IFN-gamma and IL-2 production, but a less potent costimulator of IL-4 and IL-5 production. The data suggest that T cell counterreceptors and APC costimulatory molecules act with varying efficacies at stimulating production of T cell cytokines.
Petro
T M
TM
Dept. of Oral Biology, University of Nebraska Medical Center, Lincoln, USA.
Chen
S S
SS
Panther
R B
RB
D000069594:Abatacept; D000818:Animals; D015703:Antigens, CD / Q000494:pharmacology*; D000943:Antigens, Differentiation / Q000502:physiology; D018122:B7-1 Antigen / Q000494:pharmacology*; D051940:B7-2 Antigen; D018106:CD28 Antigens / Q000502:physiology; D060908:CTLA-4 Antigen; D006224:Cricetinae; D016207:Cytokines / Q000096:biosynthesis* / Q000276:immunology; D005260:Female; D018796:Immunoconjugates; D008213:Lymphocyte Activation; D008262:Macrophage Activation; D008562:Membrane Glycoproteins / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism*
D015703:Antigens, CD; D000943:Antigens, Differentiation; D018122:B7-1 Antigen; D051940:B7-2 Antigen; D018106:CD28 Antigens; D060908:CTLA-4 Antigen; C497965:Cd86 protein, mouse; C556707:Ctla4 protein, mouse; D016207:Cytokines; D018796:Immunoconjugates; D008562:Membrane Glycoproteins; D000069594:Abatacept