Efficient transactivation of the minute virus of mice P38 promoter requires upstream binding of NS1.
The P38 promoter of the autonomous parvovirus minute virus of mice is strongly transactivated by the nonstructural protein NS1, a sequence-specific DNA-binding protein. In the context of the complete viral genome, the only unique cis-acting signals required for P38 transactivation by NS1 are the proximal Sp1 site and the TATA element. In the absence of additional upstream sequences, a dependence upon the NS1 binding site within the transactivation response region is observed. Addition of synthetic NS1 binding sites to transactivation response region deletion mutants can restore the ability of NS1 to transactivate P38, and NS1 transactivation has been directly correlated to its ability to bind upstream of the P38 promoter.
Lorson
C
C
Department of Molecular Microbiology and Immunology, University of Missouri School of Medicine, Columbia 65212, USA.
Burger
L R
LR
Mouw
M
M
Pintel
D J
DJ
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002460:Cell Line; D004279:DNA, Viral; D015967:Gene Expression Regulation, Viral; D016679:Genome, Viral; D051379:Mice; D008915:Minute Virus of Mice / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D017384:Sequence Deletion; D016329:Sp1 Transcription Factor / Q000378:metabolism; D016385:TATA Box; D015533:Transcriptional Activation; D015854:Up-Regulation; D017361:Viral Nonstructural Proteins / Q000737:chemistry / Q000378:metabolism*
D004279:DNA, Viral; C081367:NS1 protein, minute virus of mice; D016329:Sp1 Transcription Factor; D017361:Viral Nonstructural Proteins