Metastasis-promoting activity of a novel molecule, Ag 243-5, derived from mycoplasma, and the complete nucleotide sequence.
We found that mycoplasma-infected cells have a higher ability to metastasize in vivo than non-mycoplasma-infected cells. To investigate this phenomenon, we obtained a monoclonal antibody, MAb 243-5, by immunization with Mycoplasma arginini-infected RPMI 4788 cells. This MAb recognized a mycoplasmal protein with an MW of 47 kDa and completely inhibited the experimental metastasis of M. arginini-infected RPMI 4788 cells using a nude mouse model. Using this MAb, we purified a molecule called Ag 243-5 and determined the N-terminal amino acid sequence and clarified the entire nucleotide sequence of the Ag 243-5 gene. PCR analysis showed the existence of a homologous gene in Mycoplasma hyorhinis. Four sequential injections of Ag 243-5 (30 micrograms/shot) promoted the experimental metastasis of non-mycoplasma-infected RPMI 4788 cells more than 10-fold using a nude mouse model. Ag 243-5 also promoted the experimental metastasis of the non-mycoplasma-infected mouse colon cancer cell line colon 26. This metastasis-promoting effect was neutralized by MAb 243-5.
Ushio
S
S
Fujisaki Institute, Hayashibara Biochemical Laboratories, Inc., Okayama, Japan.
Iwaki
K
K
Taniai
M
M
Ohta
T
T
Fukuda
S
S
Sugimura
K
K
Kurimoto
M
M
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001426:Bacterial Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000633:toxicity*; D001483:Base Sequence; D002273:Carcinogens / Q000737:chemistry / Q000302:isolation & purification / Q000633:toxicity*; D002846:Chromatography, Affinity; D003110:Colonic Neoplasms / Q000382:microbiology* / Q000473:pathology; D004269:DNA, Bacterial / Q000032:analysis; D005260:Female; D005798:Genes, Bacterial; D008175:Lung Neoplasms / Q000556:secondary*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008969:Molecular Sequence Data; D009174:Mycoplasma / Q000737:chemistry / Q000502:physiology*; D009362:Neoplasm Metastasis; D009363:Neoplasm Proteins; D016133:Polymerase Chain Reaction; D014407:Tumor Cells, Cultured
C097682:Ag 243-5 protein, Mycoplasma arginini; D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins; D002273:Carcinogens; D004269:DNA, Bacterial; D009363:Neoplasm Proteins