Temperature-dependent dimorphism of the yeast Arxula adeninivorans Ls3.
Arxula adeninivorans Ls3 is described as an ascomycetous, arthroconidial, anamorphic, xerotolerant yeast, which was selected from wood hydrolysates in Siberia. By using minimal salt medium or yeast-extract-peptone-medium with glucose or maltose as carbon source it was shown that this yeast is able to grow at up to 48 degrees C. Increasing temperatures induce changes in morphology from the yeast phase to mycelia depending on an altered programme of gene expression. This dimorphism is an environmentally conditioned (reversible) event and the mycelia can be induced at a cultivation temperature of 45 degrees C. Depending on the morphology of strain Ls3 (yeast phase or mycelia) the secretion behaviour as well as the spectrum of polypeptides accumulated in the culture medium changed. The activities of the accumulated extracellular enzymes glucoamylase and invertase were 2 to 3 times higher in cultures grown at 45 degrees C than in those grown at 30 degrees C. While the level of the glucoamylase protein secreted from mycelia between 45 and 70 hours did not change, biochemical activity decreased after a cultivation time of 43 hours. It was shown that this effect depended on both the catabolic repression of the glucoamylase by glucose and the thermal inactivation of this enzyme in media without or with low concentrations of starch or maltose.
Wartmann
T
T
Institute of Plant Genetics and Crop Plant Research, Gatersleben, Germany.
Krüger
A
A
Adler
K
K
Duc
B M
BM
Kunze
I
I
Kunze
G
G
D001203:Ascomycota / Q000502:physiology* / Q000648:ultrastructure; D005656:Fungal Proteins / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D005087:Glucan 1,4-alpha-Glucosidase / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000378:metabolism; D008320:Maltose / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D012331:RNA, Fungal / Q000096:biosynthesis / Q000235:genetics; D013696:Temperature; D043324:beta-Fructofuranosidase
D005656:Fungal Proteins; D012331:RNA, Fungal; D008320:Maltose; D006026:Glycoside Hydrolases; D043324:beta-Fructofuranosidase; D005087:Glucan 1,4-alpha-Glucosidase; D005947:Glucose