title: Flow-injection extraction-spectrophotometric determination of bromhexine with orange IV.
abstract: An automatic flow-injection photometric method for the determination of bromhexine is proposed. The drug was determined by formation of an ion-pair with orange IV, extraction into 1,2-dichloroethane and measurement of the absorbance at 412 nm of the organic phase. A linear calibration graph was obtained at concentrations of 5 x 10(-6)-1.6 x 10(-4) M of bromhexine. Up to 40 samples h-1 can be processed with an RSD of 0.32-0.88%. The method was applied to the determination of bromhexine in blood serum and a pharmaceutical preparation.
authors.affiliation: Department of Analytical Chemistry, Faculty of Chemistry, University of Murcia, Spain.
authors.forename: T
authors.initials: T
authors.lastname: Pérez-Ruiz
authors.forename: C
authors.initials: C
authors.lastname: Martínez-Lozano
authors.forename: A
authors.initials: A
authors.lastname: Sanz
authors.forename: S
authors.initials: S
authors.lastname: Mondéjar
mesh_terms: D001391:Azo Compounds; D001964:Bromhexine / Q000032:analysis* / Q000097:blood / Q000737:chemistry; D002138:Calibration; D004396:Coloring Agents; D005025:Ethylene Dichlorides; D005100:Expectorants / Q000032:analysis* / Q000737:chemistry; D017022:Flow Injection Analysis; D006863:Hydrogen-Ion Concentration; D013056:Spectrophotometry, Ultraviolet
chemical_list: D001391:Azo Compounds; D004396:Coloring Agents; D005025:Ethylene Dichlorides; D005100:Expectorants; C024565:ethylene dichloride; D001964:Bromhexine