[Simultaneous staining with fluorescein diacetate-propidium iodide to determine isolated cochlear outer hair cell viability of guinea pig].
A simultaneous double-staining procedure using fluorescein diacetate (FDA) and propidium iodide (PI) is discribed for use in the determination of isolated cochlear outer hair cell viability. With exciter light, viable cells fluoresce bright green, while nonviable cells are bright red. In cell culture and cytotoxicity studies, double-staining with FDA-PI is a accurate method to discriminate between live and nonviable cells.
Yu
Q
Q
Union Hospital, Tongji Medical University, Wuhan.
Shi
H
H
Wang
J
J
D000818:Animals; D002470:Cell Survival; D003051:Cochlea / Q000166:cytology*; D004396:Coloring Agents; D005452:Fluoresceins; D006168:Guinea Pigs; D018072:Hair Cells, Auditory, Outer / Q000502:physiology*; D066298:In Vitro Techniques; D011419:Propidium; D013194:Staining and Labeling / Q000379:methods
D004396:Coloring Agents; D005452:Fluoresceins; D011419:Propidium; C018506:diacetylfluorescein