Separate estimation of biological and analytical variance components when quantities and reagents are unstable.
A model for reliable estimation of variance components for biological within- and between-subject variation as well as for analytical variation when both the quantity and the reagents are unstable has been established. This model was applied to the alpha-interferon receptor on human leucocytes which involves two major problems. First, the receptor has to be quantified within a few hours after blood sampling, and second, the reagents for the measurement procedure must be used within 2 weeks. For the number of receptors per cell the biological estimates of coefficients of variation were 14% for within-subject variation and 20% for between-subject variation, respectively. For the dissociation constant both estimates were zero as expected. The model is robust and applicable to other systems with unstable quantities and reagents.
Gerdes
A M
AM
Department of Clinical Chemistry, Odense University Hospital, Denmark.
Petersen
P H
PH
Hørder
M
M
Bonnevie-Nielsen
V
V
D000328:Adult; D000704:Analysis of Variance; D004355:Drug Stability; D005260:Female; D006801:Humans; D007202:Indicators and Reagents / Q000737:chemistry*; D007963:Leukocytes, Mononuclear / Q000737:chemistry* / Q000378:metabolism; D008875:Middle Aged; D008954:Models, Biological; D053633:Receptor, Interferon alpha-beta; D017471:Receptors, Interferon / Q000737:chemistry
D007202:Indicators and Reagents; D017471:Receptors, Interferon; D053633:Receptor, Interferon alpha-beta