A subpopulation of B220+ cells in murine bone marrow does not express CD19 and contains natural killer cell progenitors.
Bone marrow of both normal and rearrangement-deficient mice contains a small population of B220(CD45R)+ cells, which do not express the B lineage marker CD19. Instead, part of this population coexpresses the surface marker CD43 and lacks or expresses very low levels of heat stable antigen (HSA) and BP-1, thus representing a part of Hardy's fraction A (B220(+)-CD43+HSA-, BP-1-) of B lineage development. However, some 20-40% of these B220(+)-CD19- cells also coexpress the NK1.1 surface molecule and do not express genes like VpreB or B29 restricted to the B cell lineage. These cells respond to recombinant interleukin 2 in vitro, and develop into killer cells that can lyse the prototypic NK target tumor cell, YAC-1, as well as syngeneic normal lipopolysaccharide or concanavalin A blasts, providing they lack the surface expression of major histocompatibility complex class I molecules. The implications of these findings for studies on B lymphopoiesis are discussed. It is suggested that the CD19-specific monoclonal antibody is more reliable, as in humans, than B220(CD45R) to detect B lineage cells in mice.
Rolink
A
A
Basel Institute for Immunology, Switzerland.
ten Boekel
E
E
Melchers
F
F
Fearon
D T
DT
Krop
I
I
Andersson
J
J
D000818:Animals; D000911:Antibodies, Monoclonal; D018941:Antigens, CD19 / Q000032:analysis* / Q000276:immunology; D001483:Base Sequence; D001854:Bone Marrow Cells; D002857:Chromium / Q000378:metabolism; D002999:Clone Cells; D003602:Cytotoxicity, Immunologic; D005260:Female; D005434:Flow Cytometry; D006412:Hematopoietic Stem Cells / Q000737:chemistry* / Q000187:drug effects / Q000276:immunology; D007376:Interleukin-2 / Q000494:pharmacology; D015979:Killer Cells, Lymphokine-Activated; D007694:Killer Cells, Natural / Q000737:chemistry* / Q000187:drug effects / Q000276:immunology; D017493:Leukocyte Common Antigens / Q000032:analysis*; D016131:Lymphocyte Subsets / Q000737:chemistry* / Q000187:drug effects / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction
D000911:Antibodies, Monoclonal; D018941:Antigens, CD19; D007376:Interleukin-2; D002857:Chromium; D017493:Leukocyte Common Antigens