Induction and characterization of monoclonal anti-idiotypic antibodies to tick-borne encephalitis virus neutralizing antibody.
Seven monoclonal anti-idiotypic antibodies (anti-ID MAbs, Ab2) were generated against virus-neutralizing and hemagglutination-inhibiting monoclonal antibody (Ab1) specific for tick-borne encephalitis (TBE) virus. Six of these anti-ID MAbs inhibited the binding of Ab1 to the virus antigen, thus classifying these anti-ID antibodies as Ab2 beta or AB2 gamma. Inhibition tests with heterologous anti-TBE sera revealed that these anti-ID MAbs were not recognized by anti-TBE antibodies and therefore they do not carry an internal image of TBE virus antigen. Hence, the anti-ID MAbs may be classified as Ab2 gamma type. None of the anti-ID MAbs induced production of antiviral antibodies nor protective immunity in syngeneic Balb/c mice. Using these anti-ID MAbs four nonoverlapping idiotopes were identified on Ab1 variable region.
Kopecký
J
J
Institute of Parasitology, Czech Academy of Sciences, Ceské Budĕjovice, Czech Republic.
Krejcí
R
R
Gould
E A
EA
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000096:biosynthesis* / Q000737:chemistry; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000737:chemistry; D000914:Antibodies, Viral / Q000096:biosynthesis* / Q000737:chemistry; D003429:Cross Reactions; D004669:Encephalitis Viruses, Tick-Borne / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D018604:Epitope Mapping; D005260:Female; D007106:Immune Sera / Q000737:chemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D007106:Immune Sera