[Effect of Astragalus membranaceus on Ca2+ influx and coxsackie virus B3 RNA replication in cultured neonatal rat heart cells].
The effect of Astragalus membranaceus (AM) on Ca2+ influx across the myocardial plasma membrane and coxsackie virus B3(CVB3)-RNA replication in cultured neonatal rat heart cells infected with CVB3 was investigated. It was found that the Ca2+ influx could be inhibited significantly (P < 0.01) by AM after infection of heart cells for 48 h. In addition, when the cultured heart cells infected with CVB3 and treated with AM for 48 h, the Ca2+ influx of infected heart cells also could be inhibited by AM (P < 0.05) and the amounts of CVB3-RNA in myocytes were significantly decreased than that in infected control group (P < 0.001). These phenomena suggested that AM could exert the effects of decreasing the secondary Ca2+ damages, and improving the abnormal myocardial electric activity, and inhibiting replication of CVB3-RNA in myocardium. Thus, it is a rational choice to treat patients with AM in viral myocarditis.
Guo
Q
Q
Zhongshan Hospital, Shanghai Medical University, Shanghai.
Peng
T Q
TQ
Yang
Y Z
YZ
D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D027885:Astragalus propinquus; D001693:Biological Transport, Active / Q000187:drug effects; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D029822:Enterovirus B, Human / Q000187:drug effects* / Q000502:physiology; D006321:Heart / Q000821:virology*; D009206:Myocardium / Q000166:cytology; D012367:RNA, Viral / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014779:Virus Replication / Q000187:drug effects*
D000998:Antiviral Agents; D004365:Drugs, Chinese Herbal; D012367:RNA, Viral; D002118:Calcium