[Selection of optimal conditions for synthesis of protein A-laccase conjugates and their use in different variants of immunoenzyme analysis].
A study to determine optimal molar ratios of Protein A to laccase for the synthesis of their conjugate by periodate method is presented. No loss of enzymatic or immunological activity of the conjugate developed was observed during 6 month. The conjugate could be effectively used in various techniques of enzyme immunoassay (competitive or sandwich techniques, dot immunoblotting) for immunoglobulin G. The detection limits of the assays for immunoglobulins were better than 1 ng/ml.
Skorobogat'ko
O V
OV
Stepanova
E V
EV
Dzhafarova
A N
AN
Iaropolov
A I
AI
D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G; D042845:Laccase; D010088:Oxidoreductases / Q000737:chemistry*; D010504:Periodic Acid / Q000737:chemistry; D011993:Recombinant Fusion Proteins / Q000032:analysis / Q000138:chemical synthesis* / Q000302:isolation & purification; D013205:Staphylococcal Protein A / Q000737:chemistry*
D007074:Immunoglobulin G; D011993:Recombinant Fusion Proteins; D013205:Staphylococcal Protein A; D010504:Periodic Acid; D010088:Oxidoreductases; D042845:Laccase
Podbor optimal'nykh usloviĭ sinteza kon''iugatov belok A-lakkaza i ikh ispol'zovanie v razlichnykh variantakh immunofermentnogo analiza.