title: Protection of plasmid pBR322 DNA by flavonoids against single-stranded breaks induced by singlet molecular oxygen.
abstract: Flavonoids, the dominant colouring pigments of plants, as well as the related polyphenol tannic acid significantly inhibit single-strand breaks in plasmid pBR322 DNA induced by singlet molecular oxygen ((1)O2). This reactive species of oxygen was generated in an aqueous buffer system by the thermal dissociation of the endoperoxide of 3,3'-(1,4-naphthylene)dipropionate. Among the antioxidants examined, myricetin showed the highest protective ability, followed by tannic acid, (+)catechin, rutin, fisetin, luteolin and apigenin, when the inhibitory abilities were compared at 90 min after incubation. The protective abilities of these compounds were both time and concentration dependent. At equimolar concentrations (100 microM) the antioxidant effect of myricetin was better than that of other known antioxidants such as lipoate, alpha-tocopherol and beta-carotene. Data, when analysed in relation to the structures of various compounds, showed a rough correlation with protective abilities. Owing to the abundance of these compounds in our normal diet, they may play significant roles in preventing oxidative damage resulting from potentially deleterious (1)O2.
authors.affiliation: Radiation Biology and Biochemistry Division, Bhabha Atomic Research Center, Bombay, India.
authors.forename: T P
authors.initials: TP
authors.lastname: Devasagayam
authors.forename: M
authors.initials: M
authors.lastname: Subramanian
authors.forename: B B
authors.initials: BB
authors.lastname: Singh
authors.forename: R
authors.initials: R
authors.lastname: Ramanathan
authors.forename: N P
authors.initials: NP
authors.lastname: Das
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D004249:DNA Damage; D005419:Flavonoids / Q000737:chemistry / Q000494:pharmacology*; D015394:Molecular Structure; D009284:Naphthols / Q000737:chemistry / Q000494:pharmacology; D010100:Oxygen / Q000494:pharmacology*; D010957:Plasmids / Q000187:drug effects*; D017382:Reactive Oxygen Species / Q000737:chemistry / Q000494:pharmacology; D026082:Singlet Oxygen
chemical_list: D000975:Antioxidants; D005419:Flavonoids; D009284:Naphthols; D017382:Reactive Oxygen Species; D026082:Singlet Oxygen; C058008:3,3'-(1,4-naphthylidene)diproprionate; D010100:Oxygen