False-positive postmortem EMIT drugs-of-abuse assay due to lactate dehydrogenase and lactate in urine.
Three cases of multiple false-positive drug tests are described. Postmortem urine specimens were screened using the enzyme-multiplied immunoassay technique. All patients had proteinuria and lactic aciduria. These false-positive reactions were due to the presence of lactate dehydrogenase (LDH), lactic acid, and protein. This finding was confirmed by creating a multiple false-positive sample with a solution of LDH and lactate in 5% bovine serum albumin at pH 6.
Sloop
G
G
Department of Pathology, University of Alabama at Birmingham, USA.
Hall
M
M
Simmons
G T
GT
Robinson
C A
CA
D000328:Adult; D000661:Amphetamine / Q000652:urine; D001463:Barbiturates / Q000652:urine; D001569:Benzodiazepines / Q000652:urine; D002138:Calibration; D003042:Cocaine / Q000652:urine; D011431:Dextropropoxyphene / Q000652:urine; D017248:Enzyme Multiplied Immunoassay Technique; D005189:False Positive Reactions; D005260:Female; D006801:Humans; D013287:Illicit Drugs / Q000652:urine*; D007770:L-Lactate Dehydrogenase / Q000652:urine*; D007773:Lactates / Q000652:urine; D019344:Lactic Acid; D008297:Male; D008875:Middle Aged; D009294:Narcotics / Q000652:urine; D011180:Postmortem Changes; D011507:Proteinuria; D012710:Serum Albumin, Bovine
D001463:Barbiturates; D013287:Illicit Drugs; D007773:Lactates; D009294:Narcotics; D001569:Benzodiazepines; D012710:Serum Albumin, Bovine; D019344:Lactic Acid; D000661:Amphetamine; D007770:L-Lactate Dehydrogenase; D003042:Cocaine; D011431:Dextropropoxyphene