title: Identification of chitinase as the immunodominant filarial antigen recognized by sera of vaccinated rodents.
abstract: Acanthocheilonema viteae is a parasitic nematode of rodents. We identified the chitinase of A. viteae infective stage larvae (L3) as the main target of the humoral immune response of jirds, which were protected against challenge infection after vaccination with irradiation attenuated L3. The cDNA of the L3 chitinase has been sequenced, and the deduced amino acid sequence shows significant homologies to chitinases of Brugia malayi microfilariae, insects, yeast, bacteria, and Streptomyces sp. The protein has been characterized by monoclonal antibodies and substrate activity gels. The chitinase of L3 may contribute to degrading the nematode cuticle during molting and thus represents a target of protective immune responses in a phase where the parasite is highly vulnerable. In addition, it has been shown that a similar enzyme exists in uterine microfilariae, which probably has a role in casting the egg shell.
authors.affiliation: Division of Parasitology, University of Hohenheim, Stuttgart, Germany.
authors.forename: R
authors.initials: R
authors.lastname: Adam
authors.forename: B
authors.initials: B
authors.lastname: Kaltmann
authors.forename: W
authors.initials: W
authors.lastname: Rudin
authors.forename: T
authors.initials: T
authors.lastname: Friedrich
authors.forename: T
authors.initials: T
authors.lastname: Marti
authors.forename: R
authors.initials: R
authors.lastname: Lucius
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth / Q000276:immunology*; D001419:Bacteria / Q000201:enzymology; D001483:Base Sequence; D017178:Brugia malayi / Q000201:enzymology; D002688:Chitinases / Q000032:analysis / Q000235:genetics / Q000276:immunology*; D018076:DNA, Complementary; D004153:Dipetalonema / Q000201:enzymology* / Q000276:immunology* / Q000648:ultrastructure; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D005849:Gerbillinae / Q000276:immunology / Q000469:parasitology*; D007313:Insecta / Q000201:enzymology; D016253:Microscopy, Immunoelectron; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D013302:Streptomyces / Q000201:enzymology; D013987:Ticks / Q000469:parasitology; D014599:Uterus / Q000469:parasitology; D014611:Vaccination
chemical_list: D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth; D018076:DNA, Complementary; D002688:Chitinases