Cell autonomous expression of perlecan and plasticity of cell shape in embryonic muscle of Caenorhabditis elegans.
Perlecan, a component of the extracellular matrix (ECM), is essential for myofilament formation and muscle attachment in Caenorhabditis elegans. We show here that perlecan is a product of muscle and that it behaves in a cell autonomous fashion. That is, perlecan expressed in an individual muscle cell does not spread beyond the borders of the ECM underlying that cell. Using a polyclonal antibody that recognizes all isoforms of perlecan, we demonstrate that this protein first appears extracellularly at the comma stage (approx. 350 min) of development. We also show that during morphogenesis muscle cells have a heretofore undescribed plasticity of shape. This ability to regulate cell shape allows cells within a muscle quadrant to compensate for missing cells and to form a functional quadrant. A dramatic example of this morphological flexibility can be observed in animals in which the D blastomere has been removed by laser ablation. Such animals, lacking 20 of the 81 embryonic body wall muscle cells, can survive to become viable adult animals indistinguishable from wildtype animals. This demonstrates that the assembly of an embryo via a stereotypic lineage does not preclude a more general regulation during morphogenesis. It appears that embryos are flexible enough to immediately compensate for drastic alterations in tissue composition, a feature of development that may be of general importance during evolution.
Moerman
D G
DG
Max-Planck-Institut für Biochemie, Martinsried, Federal Republic of Germany.
Hutter
H
H
Mullen
G P
GP
Schnabel
R
R
D000818:Animals; D001757:Blastomeres; D017173:Caenorhabditis elegans / Q000166:cytology / Q000196:embryology*; D029742:Caenorhabditis elegans Proteins; D002465:Cell Movement; D048429:Cell Size; D005109:Extracellular Matrix / Q000378:metabolism*; D015801:Helminth Proteins / Q000096:biosynthesis / Q000235:genetics; D019812:Heparan Sulfate Proteoglycans; D006497:Heparan Sulfate / Q000096:biosynthesis* / Q000235:genetics; D007150:Immunohistochemistry; D053685:Laser Therapy; D008565:Membrane Proteins; D009024:Morphogenesis; D009132:Muscles / Q000166:cytology / Q000196:embryology*; D011509:Proteoglycans / Q000096:biosynthesis* / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis
D029742:Caenorhabditis elegans Proteins; D015801:Helminth Proteins; D019812:Heparan Sulfate Proteoglycans; D006497:Heparan Sulfate; D008565:Membrane Proteins; D011509:Proteoglycans; D011994:Recombinant Proteins; C082396:unc-52 protein, C elegans; C071927:perlecan