In vitro effects of ethanol on polymorphonuclear leukocyte membrane receptor expression and mobility.
The hampered inflammation and host defense seen in alcoholics may be due to impairment of functional responses of neutrophil polymorphonuclear leukocytes (PMN). We have shown that ethanol inhibits the oxidative metabolism of PMN induced by surface receptor dependent stimuli, such as N-formyl-methionyl-leucyl-phenylalanine (fMLP) and opsonized zymosan. Because the unresponsiveness might be due to reduced numbers of surface receptors, we assessed the expression of CR1, Fc-gamma, and fMLP receptors as well as membrane fluidity after treatment of PMN with ethanol in vitro. Ethanol impaired the induced expression of CR1 and fMLP receptors to 71% and 51% of control, respectively, but did not affect the resting level of CR1 nor Fc-gamma receptor expression. Furthermore, the mobility of cell membrane glycoconjugates was increased by ethanol. However, phagocytosis, a functional response dependent on membrane rheology, was unaffected. Because the results indicated an effect of ethanol on mobilization of receptors from intracellular stores, we assessed lactoferrin release, which was reduced to 59%. Thus, ethanol appeared to hamper the upregulation of PMN surface receptors or functional subsets of those stored in granules. Ethanol also increased the mobility of the cell membrane. These reactions were accompanied by reductions in the functional responses mediated by either class of receptors.
Nilsson
E
E
Department of Medicine, Karolinska Institute, Stockholm Söder Hospital, Sweden.
Halldén
G
G
Magnusson
K E
KE
Hed
J
J
Palmblad
J
J
D002478:Cells, Cultured; D000431:Ethanol / Q000494:pharmacology*; D006801:Humans; D007781:Lactoferrin / Q000378:metabolism; D008163:Luminescent Measurements; D008560:Membrane Fluidity; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism; D010587:Phagocytosis; D017463:Receptors, Complement 3b / Q000378:metabolism; D011955:Receptors, Drug / Q000378:metabolism*; D011961:Receptors, Fc / Q000378:metabolism; D044042:Receptors, Formyl Peptide; D011971:Receptors, Immunologic / Q000378:metabolism; D018000:Receptors, Peptide / Q000378:metabolism
D017463:Receptors, Complement 3b; D011955:Receptors, Drug; D011961:Receptors, Fc; D044042:Receptors, Formyl Peptide; D011971:Receptors, Immunologic; D018000:Receptors, Peptide; D000431:Ethanol; D007781:Lactoferrin