title: Antibiotic-resistance cassettes for Bacillus subtilis.
abstract: The genes encoding resistance to four different antibiotics (erythromycin, kanamycin, tetracycline and spectinomycin) were cloned in the polylinker of various Escherichia coli plasmid vectors. These cassettes can be inserted into cloned Bacillus subtilis (Bs) genes and used to create tagged chromosomal disruptions after recombination into Bs and selection in the presence of the appropriate antibiotic.
authors.affiliation: Institut de Biologie Physico-Chimique, Paris, France.
authors.forename: A M
authors.initials: AM
authors.lastname: Guérout-Fleury
authors.forename: K
authors.initials: K
authors.lastname: Shazand
authors.forename: N
authors.initials: N
authors.lastname: Frandsen
authors.forename: P
authors.initials: P
authors.lastname: Stragier
mesh_terms: D001412:Bacillus subtilis / Q000235:genetics*; D001483:Base Sequence; D004352:Drug Resistance, Microbial; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D010957:Plasmids