The regulation of acetylated microtubules during outgrowth from cultured neurons of the snail, Helisoma.
Axonal stumps of cultured Helisoma trivolvis neurons express abundant acetylated microtubules, as a subset of total microtubules. Label completely disappears from the axonal remnants within approximately 1 day, and reappears in newly extended neurites over the course of the next 3-4 days, first in the proximal neurite as short, isolated segments. Acetylated microtubules occur in the neuritic shaft, but never in growth cones or membranous veils. Thus, acetylated microtubules are very labile to the signals generated by axotomy, and their proximodistal re-expression occurs at well separated sites within the neurite as it matures.
Hadley
R D
RD
Department of Medicine, Medical University of South Carolina, Charleston 29425, USA.
Miller
J D
JD
D000107:Acetylation; D000818:Animals; D001369:Axons / Q000378:metabolism; D002478:Cells, Cultured; D008870:Microtubules / Q000378:metabolism*; D016501:Neurites / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism* / Q000648:ultrastructure; D012908:Snails