title: Healing of reopened-and-sutured radial keratotomy wounds.
abstract: We designed a study to evaluate healing in reopened-and-sutured (RAS) keratotomy wounds to determine the efficacy of reoperations in treating radial keratotomy overcorrections. Using light and transmission electron microscopy, we compared stromal scar tissue organization (transverse fibroblast orientation and collagen fiber continuity across the wound) in RAS wounds and in sutured and unsutured control wounds in 18 monkey eyes one to nine weeks after surgery. Wound healing morphology of RAS wounds varied with the interval between reoperation and termination of the experiment. Scar tissue organization was sagittal at one week postoperatively, transverse in the anterior and mid regions after four weeks, and transverse over the entire wound after nine weeks. Sutured wounds showed a similar pattern of healing, although transverse scar tissue organization was restricted to the anterior and mid regions in the late healing phases. In contrast, unsutured wounds showed a temporary, transverse scar tissue organization over the entire wound depth at two to four weeks and a progressive reorientation of the mid and posterior scar tissue sagittal to the wound at later intervals. The results suggest that reopening and suturing keratotomy incisions to treat radial keratotomy overcorrections may be effective through a myopic shift induced by sutured wound apposition and long-term wound remodeling, contraction, or both.
authors.affiliation: Department of Ophthalmology, University of Nijmegen, The Netherlands.
authors.forename: G R
authors.initials: GR
authors.lastname: Melles
authors.forename: P S
authors.initials: PS
authors.lastname: Binder
authors.forename: W H
authors.initials: WH
authors.lastname: Beekhuis
authors.forename: R H
authors.initials: RH
authors.lastname: Wijdh
authors.forename: F J
authors.initials: FJ
authors.lastname: Rietveld
mesh_terms: D000818:Animals; D003315:Cornea / Q000473:pathology / Q000601:surgery*; D003319:Corneal Stroma / Q000648:ultrastructure; D004848:Epithelium / Q000648:ultrastructure; D005347:Fibroblasts / Q000648:ultrastructure; D000882:Haplorhini; D007646:Keratotomy, Radial; D012086:Reoperation; D013536:Suture Techniques; D014945:Wound Healing