title: Polysulphone inhibits final differentiation steps of osteogenesis in vitro.
abstract: Biocompatibility is an important factor in the development of orthopedic implants as well as in the development of new tissue culture devices. Polysulphone has been used for orthopedic implants because of its mechanical properties, ease of sterilization, molding capacity, and biocompatibility. Therefore, polysulphone has been chosen as the prime material for the construction of tissue culture devices to be used for the cultivation of osteogenic cells (preosteoblast-like MN7 cells and primary bone marrow fragments), as well as complete fetal long bone explants under space flight conditions. Whereas polysulphone did not interfere with the proliferation in early stages of bone-forming cells, we show that leachable factors within the polysulphone polymer prevented the final steps of matrix formation as measured by collagen synthesis and matrix mineralization. These data argue against polysulphone as a material for orthopedic implants.
authors.affiliation: Department of Oral Cell Biology, Academic Center for Dentistry Amsterdam (ACTA), The Netherlands.
authors.forename: J J
authors.initials: JJ
authors.lastname: van Loon
authors.forename: J
authors.initials: J
authors.lastname: Bierkens
authors.forename: J
authors.initials: J
authors.lastname: Maes
authors.forename: G E
authors.initials: GE
authors.lastname: Schoeters
authors.forename: D
authors.initials: D
authors.lastname: Ooms
authors.forename: B Z
authors.initials: BZ
authors.lastname: Doulabi
authors.forename: J P
authors.initials: JP
authors.lastname: Veldhuijzen
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D001854:Bone Marrow Cells; D001842:Bone and Bones; D002113:Calcification, Physiologic / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D046508:Culture Techniques / Q000295:instrumentation / Q000379:methods; D005269:Femur; D005333:Fetus; D008682:Metatarsal Bones; D051379:Mice; D008807:Mice, Inbred BALB C; D009924:Organ Culture Techniques / Q000379:methods; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects; D010012:Osteogenesis / Q000187:drug effects / Q000502:physiology*; D011108:Polymers / Q000494:pharmacology*; D019736:Prostheses and Implants; D013450:Sulfones / Q000494:pharmacology*
chemical_list: D001672:Biocompatible Materials; D011108:Polymers; D013450:Sulfones; C017662:polysulfone P 1700