DNA fingerprinting by random amplified polymorphic DNA and restriction fragment length polymorphism is useful for yeast typing.
Random amplified polymorphic DNA (RAPD) analysis was applied to genomic DNA from nineteen yeast strains belonging to the genera Saccharomyces and Zygosaccharomyces. Results obtained with five primers indicated that this technique is a powerful tool for yeast differentiation and identification. The data were consistent with those derived from restriction fragment length polymorphism (RFLP) using two S. cerevisiae DNA probes. We conclude that RAPD fingerprinting, combined with the analysis of RFLP, can provide unambiguous type assignment in yeasts.
Paffetti
D
D
Dipartimento di Biologia Animale e Genetica Leo Pardi, Università degli Studi, Firenze, Italy.
Barberio
C
C
Casalone
E
E
Cavalieri
D
D
Fani
R
R
Fia
G
G
Mori
E
E
Polsinelli
M
M
D004269:DNA, Bacterial / Q000737:chemistry* / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D066298:In Vitro Techniques; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D019105:Random Amplified Polymorphic DNA Technique; D012440:Saccharomyces / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification
D004269:DNA, Bacterial