Upregulation of insulin-like growth factor receptor gene in experimental diabetic rat glomeruli.
Renal hypertrophy is a characteristic and early manifestation of diabetes in human and experimental animals. We examined the precise distribution of insulin-like growth factor-I (IGF-I) receptor mRNA in the experimental diabetic rat kidney using a nonradioactive in situ hybridization technique. Expression of IGF-I receptor mRNA was rarely seen in the glomeruli of control rats. IGF-I receptor mRNA was detected after induction of diabetes in glomerular mesangial, visceral epithelial, and parietal epithelial cells. The number of IGF-I receptor mRNA-positive cells in a glomerulus increased significantly at 4 weeks as compared with the control rats. Overexpression of IGF-I receptor in glomerular cells may contribute to the glomerular hypertrophy in diabetic nephropathy.
Sugimoto
H
H
Third Department of Internal Medicine, Okayama University Medical School, Japan.
Shikata
K
K
Makino
H
H
Ota
K
K
Ota
Z
Z
D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism* / Q000473:pathology; D015870:Gene Expression; D006801:Humans; D006984:Hypertrophy; D007678:Kidney Glomerulus / Q000378:metabolism* / Q000473:pathology; D007700:Kinetics; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D051381:Rats; D017526:Receptor, IGF Type 1 / Q000096:biosynthesis*; D013997:Time Factors; D015854:Up-Regulation
D012333:RNA, Messenger; D017526:Receptor, IGF Type 1