title: Recruitment of co-metabolic enzymes for environmental detoxification of organohalides.
abstract: Polyhalogenated compounds are often environmentally persistent and toxic to mammals. Microorganisms that metabolize these compounds can detoxify contaminated environments. Different biochemical mechanisms are used to metabolize polyhalogenated compounds, but few naturally occurring bacteria have this capability. A recombinant bacterium was constructed to metabolize polyhalogenated compounds to nonhalogenated end products. Seven genes were expressed in Pseudomonas putida G786 to biosynthesize cytochrome P450CAM and toluene dioxygenase. Cytochrome P450CAM catalyzed reductive dechlorinated reactions and toluene dioxygenase catalyzed oxidative dechlorination. With pentachloroethane, reductive dechlorination yielded trichloroethylene, which was further oxidized to formate and glyoxylate. The sequential action of cytochrome P450CAM and toluene dioxygenase with polyhalogenated compounds constitutes a novel engineered metabolic pathway.
authors.affiliation: Department of Biochemistry, University of Minnesota, St. Paul 55108, USA.
authors.forename: L P
authors.initials: LP
authors.lastname: Wackett
mesh_terms: D001419:Bacteria / Q000235:genetics / Q000378:metabolism*; D001673:Biodegradation, Environmental; D019475:Camphor 5-Monooxygenase; D002713:Chlorine / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics / Q000378:metabolism; D004785:Environmental Pollutants / Q000378:metabolism*; D015870:Gene Expression; D006846:Hydrocarbons, Halogenated / Q000378:metabolism*; D006899:Mixed Function Oxygenases / Q000235:genetics / Q000378:metabolism; D010084:Oxidation-Reduction; D016958:Pseudomonas putida / Q000235:genetics / Q000378:metabolism; D011995:Recombination, Genetic
chemical_list: D004785:Environmental Pollutants; D006846:Hydrocarbons, Halogenated; D002713:Chlorine; D003577:Cytochrome P-450 Enzyme System; D006899:Mixed Function Oxygenases; D019475:Camphor 5-Monooxygenase