title: Contaminated wounds: infection rates with subcutaneous sutures.
abstract: OBJECTIVE
  To determine whether buried absorbable subcutaneous sutures (BASS) increase the infection rate in irrigated contaminated wounds.
  
  
  METHODS
  This was a randomized, prospective trial in a rat model, with the histologist blinded to treatment group. A single 2-cm dorsal incision was made on each of 30 anesthetized 250-g Sprague-Dawley rats and inoculated with approximately 10(8) organisms of Staphylococcus aureus. After irrigation, 15 wounds were closed with running 4-0 nylon transdermal sutures, and 15 were closed with three interrupted 4-0 coated polyglactin 910 (Vicryl) subcuticular sutures (BASS) and running 4-0 nylon transdermal sutures. On day 7, wounds were scored on a scale of 0 to 3 in six categories: inflammatory infiltrates, fibroplasia and capillary proliferation, necrosis, exudates, giant cells, and edema. The possible range for the cumulative wound score was 0 (no inflammation) to 18 (severe inflammation and infection).
  
  
  RESULTS
  The median total wound score in wounds closed with BASS was 14 (range, 7 to 16); it was 8 (range, 5 to 15) for wounds closed without BASS (P = .0004). The subscores for inflammation, necrosis, exudate, and edema were also significantly higher in wounds closed with BASS.
  
  
  CONCLUSIONS
  BASS increase the infection rate and the degree of inflammation in contaminated wounds, despite through irrigation.
authors.affiliation: Department of Emergency Medicine, East Carolina University School of Medicine, Greenville, North Carolina, USA.
authors.forename: P H
authors.initials: PH
authors.lastname: Mehta
authors.forename: K A
authors.initials: KA
authors.lastname: Dunn
authors.forename: J F
authors.initials: JF
authors.lastname: Bradfield
authors.forename: P E
authors.initials: PE
authors.lastname: Austin
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D009757:Nylons / Q000009:adverse effects*; D011098:Polyglactin 910 / Q000009:adverse effects*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012720:Severity of Illness Index; D016037:Single-Blind Method; D013203:Staphylococcal Infections / Q000209:etiology* / Q000473:pathology; D013536:Suture Techniques / Q000009:adverse effects*; D013537:Sutures / Q000009:adverse effects*; D007507:Therapeutic Irrigation; D014946:Wound Infection / Q000209:etiology* / Q000473:pathology
chemical_list: D009757:Nylons; D011098:Polyglactin 910