Class D and E tetracycline resistance determinants in gram-negative bacteria from catfish ponds.
DNA probes were used to examine tetracycline-resistant Gram-negative bacteria (281 strains representing eight species) from catfish ponds. The isolates, which did not previously hybridize with the Tet A, B and C determinants, were examined for the presence of tetracycline-resistance Tet D and Tet E determinants. The distribution of the Tet D determinant ranged from 0 to 83%, depending on the bacterial species. Tet E was found only in 69% of Aeromonas hydrophila isolates. Eighteen per cent failed to hybridize with any of the five Tet determinants.
DePaola
A
A
Gulf Coast Seafood Laboratory, Food and Drug Administration, Dauphin Island, AL 36528, USA.
Roberts
M C
MC
D000818:Animals; D002397:Catfishes / Q000382:microbiology*; D015342:DNA Probes; D004926:Escherichia coli / Q000235:genetics / Q000302:isolation & purification; D005618:Fresh Water / Q000382:microbiology; D006090:Gram-Negative Bacteria / Q000235:genetics* / Q000302:isolation & purification*; D007422:Intestines / Q000382:microbiology*; D010957:Plasmids; D013753:Tetracycline Resistance / Q000235:genetics*
D015342:DNA Probes