Identification of scavenger receptor SR-BI as a high density lipoprotein receptor.
High density lipoprotein (HDL) and low density lipoprotein (LDL) are cholesterol transport particles whose plasma concentrations are directly (LDL) and inversely (HDL) correlated with risk for atherosclerosis. LDL catabolism involves cellular uptake and degradation of the entire particle by a well-characterized receptor. HDL, in contrast, selectively delivers its cholesterol, but not protein, to cells by unknown receptors. Here it is shown that the class B scavenger receptor SR-BI is an HDL receptor. SR-BI binds HDL with high affinity, is expressed primarily in liver and nonplacental steroidogenic tissues, and mediates selective cholesterol uptake by a mechanism distinct from the classic LDL receptor pathway.
Acton
S
S
Department of Biology, Massachusetts Institute of Technology, Cambridge 02139, USA.
Rigotti
A
A
Landschulz
K T
KT
Xu
S
S
Hobbs
H H
HH
Krieger
M
M
D000311:Adrenal Glands / Q000378:metabolism; D000818:Animals; D018955:CD36 Antigens / Q000235:genetics / Q000378:metabolism*; D016466:CHO Cells; D002352:Carrier Proteins; D002784:Cholesterol / Q000378:metabolism; D002788:Cholesterol Esters / Q000378:metabolism*; D006224:Cricetinae; D005260:Female; D005456:Fluorescent Dyes / Q000378:metabolism; D008075:Lipoproteins, HDL / Q000378:metabolism*; D008099:Liver / Q000378:metabolism; D008565:Membrane Proteins; D051379:Mice; D008969:Molecular Sequence Data; D010053:Ovary / Q000378:metabolism; D016601:RNA-Binding Proteins; D011971:Receptors, Immunologic; D011973:Receptors, LDL / Q000378:metabolism; D018110:Receptors, Lipoprotein / Q000378:metabolism*; D051116:Receptors, Scavenger; D051122:Scavenger Receptors, Class B; D013843:Thiazines / Q000378:metabolism; D014162:Transfection
D018955:CD36 Antigens; D002352:Carrier Proteins; D002788:Cholesterol Esters; D005456:Fluorescent Dyes; C059714:HDL cholesteryl ester; D008075:Lipoproteins, HDL; D008565:Membrane Proteins; D016601:RNA-Binding Proteins; D011971:Receptors, Immunologic; D011973:Receptors, LDL; D018110:Receptors, Lipoprotein; D051116:Receptors, Scavenger; C454739:Scarb1 protein, mouse; D051122:Scavenger Receptors, Class B; D013843:Thiazines; C053505:fluorescent dye D 282; C031616:high density lipoprotein receptors; C075197:high density lipoprotein binding protein; C014409:cholesteryl oleate; D002784:Cholesterol