No evidence for allelic association between schizophrenia and a polymorphism determining high or low catechol O-methyltransferase activity.
OBJECTIVE
Catechol O-methyltransferase (COMT) inactivates catecholamines by methylating their m-hydroxy group. Some previous studies using biochemical methods have found higher levels of COMT activity in schizophrenic patients. Recently, the genetic polymorphism that underlies variation in COMT activity, which results in the creation of a NlaIII restriction site in the low-activity allele, has been elucidated.


METHODS
This study investigated this polymorphism in 78 unrelated schizophrenic patients and 78 comparison subjects matched for age and ethnicity. High-molecular-weight DNA was isolated from lymphocytes with routine procedures, and each individual was typed for high and low COMT activity.


RESULTS
The frequency of the NlaIII polymorphism was 0.51 in the schizophrenic patients and 0.53 in the comparison subjects, and no significant allelic or genotypic associations were observed.


CONCLUSIONS
There was no evidence for variation in COMT activity between a group of schizophrenic patients and matched comparison subjects.
Daniels
J K
JK
Department of Psychological Medicine, University of Wales College of Medicine, Cardiff.
Williams
N M
NM
Williams
J
J
Jones
L A
LA
Cardno
A G
AG
Murphy
K C
KC
Spurlock
G
G
Riley
B
B
Scambler
P
P
Asherson
P
P
McGuffin
P
P
Owen
M J
MJ
D000483:Alleles; D001483:Base Sequence; D002394:Catechol O-Methyltransferase / Q000235:genetics*; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D012150:Polymorphism, Restriction Fragment Length; D012559:Schizophrenia / Q000201:enzymology / Q000235:genetics*
D002394:Catechol O-Methyltransferase