Aberrant gene for E1 Tor hemolysin from Vibrio cholerae non-O1, N037.
Vibrio cholerae non-O1 strain N037 produced a hemolysin (NO37-Hly) which was antigenically similar to E1 Tor hemolysin (E1 Tor-Hly) but different in molecular size, hemolytic activity, and glucose binding capacity. In the gene encoding NO37-Hly, a 4-bp insertion into the structural gene for E1 Tor-Hly (hlyA) was found. The insertion in a shift of codon frames generating a new stop codon in the downstream region. NO37-Hly was a truncated product of E1 Tor-Hly sharing 90% of the N terminal region. This suggested that the 10% C-terminal region of E1 Tor-Hly is needed for the maximal hemolytic activity and glucose binding capacity.
Honma
Y
Y
Department of Bacteriology, Faculty of Medicine, University of the Ryukyus, Okinawa, Japan.
Yamamoto
K
K
Iwanaga
M
M
D000595:Amino Acid Sequence; D001427:Bacterial Toxins / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular; D005798:Genes, Bacterial / Q000235:genetics; D006460:Hemolysin Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D014734:Vibrio cholerae / Q000235:genetics*
D001427:Bacterial Toxins; D006460:Hemolysin Proteins