title: Characterization of a distinct subpopulation of bovine gamma delta T cells.
abstract: A population of mononuclear cytotoxic cells from peripheral blood leucocytes of cattle showed no usual markers of B and T lymphocytes. However, it could be allocated to a previously unreported gamma delta T cell compartment. This assumption was suggested by: 1. The surface expression of CD3; 2. PCR amplification of the C delta TcR gene from cDNA; and 3. The detection of peripheral blood precursors expressing the workshop cluster (WC) 1 marker of bovine gamma delta T cells. These cells are recognized by murine monoclonal antibodies (mAbs) 5D4, 1E7, 6F9 and 8D7, raised in the authors' laboratory. The above mAbs also identify distinct groups of cells in thymus, spleen, lymph nodes and about 1% of uncultured PBL. The most diffuse infiltration of such cells was shown in the small intestine, as both intraepithelial and lamina propria lymphocytes. Mucosal homing activity was confirmed by immunoperoxidase staining on tongue and pharynx sections of healthy cattle.
authors.affiliation: Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia, Brescia, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Amadori
authors.forename: I L
authors.initials: IL
authors.lastname: Archetti
authors.forename: R
authors.initials: R
authors.lastname: Verardi
authors.forename: C
authors.initials: C
authors.lastname: Berneri
mesh_terms: D000818:Animals; D001483:Base Sequence; D002417:Cattle / Q000276:immunology*; D005260:Female; D016694:Gene Rearrangement, delta-Chain T-Cell Antigen Receptor; D015334:Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000662:veterinary; D016176:T-Lymphocyte Subsets / Q000737:chemistry / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000737:chemistry / Q000276:immunology*