Use of a new oligonucleotide probe for detection of colonization factor antigen III gene in enterotoxigenic Escherichia coli.
An alkaline phosphatase-labeled 30-mer oligonucleotide probe was designed to detect the gene for pilus colonization factor antigen III (CFA/III) of the human type of enterotoxigenic Escherichia coli (ETEC). The CFA/III probe was used to identify CFA/III-producing ETEC among 303 Escherichia coli obtained from subjects with traveler's diarrhea. Six isolates positive for the CFA/III gene were found. This result was confirmed immunologically by using a specific monoclonal antibody developed against CFA/III. These six isolates, isolated from travelers returning from India, Pakistan and China, were all positive for the gene of heat-labile enterotoxin and possessed an identical serotype (025:H-).
Taniguchi
T
T
Department of Bacterial Infections, Osaka University, Japan.
Uchima-Senaga
N F
NF
Takarada
Y
Y
Shibata
S
S
Tsukamoto
T
T
Yamamoto
K
K
Honda
T
T
D000942:Antigens, Bacterial / Q000032:analysis / Q000235:genetics*; D001426:Bacterial Proteins / Q000032:analysis / Q000235:genetics*; D015373:Bacterial Typing Techniques; D001483:Base Sequence; D002681:China; D003967:Diarrhea / Q000382:microbiology*; D004926:Escherichia coli / Q000235:genetics / Q000276:immunology*; D036781:Fimbriae Proteins; D006801:Humans; D007194:India; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D010154:Pakistan; D012680:Sensitivity and Specificity; D014195:Travel
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D015345:Oligonucleotide Probes; C022924:colonization factor antigens; D036781:Fimbriae Proteins