title: Hemodialysis with cellulose membranes primes the neutrophil oxidative burst.
abstract: Hemodialysis with cellulose membranes causes a complement-mediated neutropenia. Changes in neutrophil function have also been reported; however, it is unclear if these changes indicate a direct effect of the membrane on neutrophils or if they are a consequence of the neutropenia. We tested the hypothesis that neutrophil oxidative burst activity is enhanced during dialysis with cellulose membranes. Resting and Staphylococcus aureus-stimulated H2O2 production were determined predialysis and in blood entering and leaving the dialyzer during the first 30 min of dialysis and in blood leaving the membrane module in a single-pass on-line model of hemodialysis. Resting H2O2 production increased slightly but significantly during the first 30 min of dialysis. Transit of neutrophils through the dialyzer caused a marked increase in stimulated H2O2 production, indicating priming of the oxidative burst. However, priming was limited to the first 5 min of dialysis before the onset of neutropenia. In contrast, stimulation and priming of H2O2 production persisted throughout 30 min of single-pass on-line perfusion. These results indicate that cellulose membranes both stimulate and prime neutrophil oxidative burst activity but that these effects are partially obscured by neutropenia.
authors.affiliation: Department of Medicine, School of Medicine, University of Louisville, Kentucky 40292, USA.
authors.forename: R A
authors.initials: RA
authors.lastname: Ward
authors.forename: K R
authors.initials: KR
authors.lastname: McLeish
mesh_terms: D000328:Adult; D000368:Aged; D002482:Cellulose; D003167:Complement Activation; D005260:Female; D006801:Humans; D006861:Hydrogen Peroxide / Q000097:blood*; D007674:Kidney Diseases / Q000628:therapy; D008297:Male; D008567:Membranes, Artificial; D008875:Middle Aged; D009503:Neutropenia / Q000209:etiology / Q000378:metabolism; D009504:Neutrophils / Q000166:cytology / Q000502:physiology*; D010587:Phagocytosis; D006435:Renal Dialysis; D016897:Respiratory Burst / Q000502:physiology*; D013211:Staphylococcus aureus
chemical_list: D008567:Membranes, Artificial; D002482:Cellulose; D006861:Hydrogen Peroxide