A novel human serum lectin with collagen- and fibrinogen-like domains that functions as an opsonin.
Collectins are C-type animal lectins with both collagenous and carbohydrate recognition domains and are involved in the first line host defense against pathogens. We report here a novel Ca(2+)-dependent and GlcNAc-binding lectin consisting of subunits of 35 kDa (P35) with a collagen-like sequence. When P35 is isolated from human serum, it forms a homopolymer by means of intermolecular disulfide bonding, as is the case with collectins. P35 cDNA was cloned from a human liver cDNA library, and the deduced amino acid sequence of 313 residues revealed that the mature form of P35 consists mainly of collagen- and fibrinogen-like domains. The latter contained two potential Ca(2+)-binding sites that may be involved in carbohydrate binding. The overall sequence of P35 was highly homologous to porcine ficolins alpha and beta. Northern blots of various human tissues showed that the major product of the 1.3-kilobase-long P35 transcript is expressed in liver. P35 enhanced phagocytosis of Salmonella typhimurium by neutrophils, suggesting an opsonic effect via the collagen region. P35 was found to bind to GlcNAc-conjugated bovine serum albumin, a neoglycoprotein, as well as to neoglycolipids containing complex-type oligosaccharides derived from glycoproteins, suggesting that P35 recognizes GlcNAc residues such as those found in microbial glycoconjugates and complex-type oligosaccharides. Therefore, P35 represents a new type of GlcNAc-binding lectin with structural and functional similarities to collectins involved in innate immunity.
Matsushita
M
M
Department of Biochemistry, Fukushima Medical College, Japan.
Endo
Y
Y
Taira
S
S
Sato
Y
Y
Fujita
T
T
Ichikawa
N
N
Nakata
M
M
Mizuochi
T
T
D000117:Acetylglucosamine / Q000378:metabolism; D000595:Amino Acid Sequence; D001212:Asialoglycoproteins / Q000378:metabolism; D001483:Base Sequence; D001665:Binding Sites; D015152:Blotting, Northern; D002118:Calcium / Q000378:metabolism; D002352:Carrier Proteins / Q000097:blood* / Q000235:genetics / Q000276:immunology; D003001:Cloning, Molecular; D003094:Collagen / Q000378:metabolism*; D018076:DNA, Complementary; D060748:Fetuins; D005340:Fibrinogen / Q000378:metabolism*; D006801:Humans; D037102:Lectins / Q000097:blood* / Q000235:genetics / Q000276:immunology; D008351:Mannans / Q000378:metabolism; D008969:Molecular Sequence Data; D009504:Neutrophils / Q000276:immunology / Q000378:metabolism; D009895:Opsonin Proteins / Q000378:metabolism*; D010587:Phagocytosis; D012486:Salmonella typhimurium / Q000276:immunology; D017386:Sequence Homology, Amino Acid; D000509:alpha-Fetoproteins / Q000378:metabolism; D000097604:Ficolins
D000117:Acetylglucosamine; D001212:Asialoglycoproteins; D002118:Calcium; D002352:Carrier Proteins; D003094:Collagen; D018076:DNA, Complementary; D060748:Fetuins; D005340:Fibrinogen; D037102:Lectins; D008351:Mannans; D009895:Opsonin Proteins; D000509:alpha-Fetoproteins; D000097604:Ficolins; C017029:asialofetuin