title: Organization of the vaccinia envelope and relationship to the structure of intracellular mature virions.
abstract: Protrusions covering the surface of intracellular mature virions (IMV), termed by us surface tubular elements (STE), are released in a quasi-intact form during stripping of the envelope. The concentrated, reproducibly isolable STE were shown to contain the 58-kDa 4c polypeptide and prominent protein antigens residing at the surface of IMV. The major core protein 4b, identified as a minor contaminant of STE, presumably became detached along with STE during the shearing off process. Antibodies against protein 4b became specifically bound to the surface of isolated cores, where a palisade layer of rodlets occurs. The same antibodies absorbed onto isolated STE where similar rodlets were evident. Based on the new observations we constructed a model of the organization of the IMV envelope and its relationship to the core.
authors.affiliation: Department of Microbiology and Immunology, University of Western Ontario, London, Canada.
authors.forename: S
authors.initials: S
authors.lastname: Wilton
authors.forename: A R
authors.initials: AR
authors.lastname: Mohandas
authors.forename: S
authors.initials: S
authors.lastname: Dales
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D000956:Antigens, Viral / Q000276:immunology; D007739:L Cells; D051379:Mice; D014616:Vaccinia virus / Q000502:physiology / Q000648:ultrastructure*; D014758:Viral Core Proteins / Q000276:immunology / Q000502:physiology / Q000648:ultrastructure; D014759:Viral Envelope Proteins / Q000302:isolation & purification / Q000502:physiology / Q000648:ultrastructure*; D014771:Virion / Q000502:physiology / Q000648:ultrastructure
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D014758:Viral Core Proteins; D014759:Viral Envelope Proteins