[HPLC method for determination of lorcainide hydrochloride in human serum].
An analytical method was established for determination of lorcainide hydrochloride concentration in serum using high performance liquid chromatography (HPLC). The stainless steel column was 200 mm x 5.0 mm ID, packed with YWG C18H37, 5 microns. The mobile phase consisted of methanol-water-0.625 mol.L-1 ammonium acetate. (86:13:1 v/v), adjusted to the pH 8.0 with ammonium hydroxide. The flow rate was 1 ml.min-1, using diltiazem as internal standard. Chromatography was performed with ultraviolet detector at 226 nm. The recoveries of lorcainide hydrochloride at 40, 200 and 400 micrograms.L-1 were 95.85%, 100.63% and 100.09%, respectively. The within-day and day-to-day RSD of the determinations at concentrations of 100, 200, 400 and 800 micrograms.L-1 were less than 7%. Linear calibration curve for lorcainide hydrochloride was measured within the range of 20 to 800 micrograms.L-1 with correlation coefficient of 0.9996. The detection limit was 5 micrograms.L-1 in serum. The HPLC method described is suitable for clinical monitoring and pharmacokinetic study of lorcainide hydrochloride.
Luo
X
X
Department of Clinical Pharmacology, Tongji Medical University, Wuhan.
Zeng
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FD
D000328:Adult; D000889:Anti-Arrhythmia Agents / Q000097:blood*; D044482:Benzeneacetamides; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D008297:Male; D010880:Piperidines / Q000097:blood*
D000889:Anti-Arrhythmia Agents; D044482:Benzeneacetamides; D010880:Piperidines; C015970:lorcainide