[Enhanced immune functions and antitumor activity of fibroblast-mediated interleukin-2 gene therapy].
The aim of the present study was to establish fibroblastmediated IL-2 gene therapy and to observe its antitumor effect in the mouse tumor model. The IL-2 gene-transfected fibroblasts (NIH3T3-IL-2+) secreting high level of IL-2 were encapsulated with collagen and then implanted i.p. into mice. Certain level of IL-2 could be detected in murine serum for some periods, and the splenic proliferation, NK and LAK activities, cytokine production (IFN-v, TNF, IL-2) were enhanced significantly. It was of great importance that the high endogenous LAK activity was induced. The significant therapeutic effect of i.p. implantation of NIH3T3-IL-2+ on ascitic liver carcinoma-bearing mice was observed. The better therapeutic results could be achieved. NIH3T3-IL-2+ cells were i.p. implanted in combination with i.p. injection of LAK cells. These results demonstrated that fibroblast--mediated IL-2 gene therapy has potent antitumor effect via augmentation of immune functions and the antitumor effect will be more obvious when IL-2 gene therapy is used along with the adoptive transfer of LAK cells.
Cao
X T
XT
Department of Immunology, Second Military Medical University, Shanghai.
Zhang
W P
WP
Tao
Q
Q
D016475:3T3 Cells / Q000276:immunology; D000818:Animals; D005347:Fibroblasts / Q000276:immunology; D015316:Genetic Therapy / Q000379:methods; D016219:Immunotherapy, Adoptive; D007376:Interleukin-2 / Q000235:genetics*; D015979:Killer Cells, Lymphokine-Activated / Q000276:immunology; D007694:Killer Cells, Natural / Q000276:immunology; D008114:Liver Neoplasms, Experimental / Q000276:immunology / Q000628:therapy*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D014162:Transfection
D007376:Interleukin-2