H2O2 determination in rat lens: chemiluminescent versus radioisotopic methods.
Hydrogen peroxide levels have been determined in rat lenses by using two methods, a chemiluminescent and a radioisotopic method. The average content was found to be 155 +/- 20 and 127 +/- 18 nmol/g wet weight of the tissue, respectively, by the two methods. The reaction of H2O2 with dichlorophenol-indophenol in the presence of peroxidase was also studied. However, this was found to be less suitable. The results of the chemiluminescent determinations are similar to the results with the radioisotopic methods demonstrating the feasibility of determining H2O2 by chemiluminescence measurement as well.
Devamanoharan
P S
PS
Department of Ophthalmology, University of Maryland at Baltimore 21201, USA.
Varma
S D
SD
D015086:2,6-Dichloroindophenol; D000818:Animals; D002250:Carbon Radioisotopes; D005240:Feasibility Studies; D006861:Hydrogen Peroxide / Q000032:analysis*; D007202:Indicators and Reagents; D007908:Lens, Crystalline / Q000737:chemistry*; D008163:Luminescent Measurements; D016877:Oxidants / Q000032:analysis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results
D002250:Carbon Radioisotopes; D007202:Indicators and Reagents; D016877:Oxidants; D006861:Hydrogen Peroxide; D015086:2,6-Dichloroindophenol