Comparison of SHARP signal system and Southern blot hybridization analysis for detection of cytomegalovirus in clinical specimens by PCR.
Thirty cytomegalovirus cell culture-positive samples were tested by the SHARP Signal System. Twenty-seven specimens (100% agreement) were identified by both methods. The SHARP Signal System is rapid (4 h), easy to perform, and potentially adaptable to automation.
Espy
M J
MJ
Division of Clinical Microbiology, Mayo Clinic and Foundation, Rochester, Minnesota 55905, USA.
Smith
T F
TF
D001483:Base Sequence; D015139:Blotting, Southern; D003586:Cytomegalovirus Infections / Q000175:diagnosis*; D006801:Humans; D007124:Immunoenzyme Techniques / Q000295:instrumentation*; D008163:Luminescent Measurements; D008969:Molecular Sequence Data; D016377:Organ Transplantation / Q000009:adverse effects; D016133:Polymerase Chain Reaction / Q000295:instrumentation*; D015347:RNA Probes; D011933:Reagent Kits, Diagnostic; D015203:Reproducibility of Results; D014766:Viremia / Q000175:diagnosis*
D015347:RNA Probes; D011933:Reagent Kits, Diagnostic