Properties and regulation of ion channels in MDCK cells.
The MDCK cell has proven to be a useful model cell line for the study of properties and regulation of renal epithelial ion channels. Patch clamp studies disclosed the existence of several K+ channels and of a Cl- channel, and their regulation by hormones, cell volume, trace elements and drugs. Most hormones affect K+ channels at least in part by increasing cytosolic Ca2+. However, indirect evidence points to additional mechanisms contributing to K+ channel activation. Cell swelling activates both K+ channels and unselective anion channels. ICln, a protein cloned from MDCK cells, is either a Cl- channel or a regulator of thereof. ICln is up-regulated by cellular acidification and is crucial for rapid regulatory cell volume decrease.
Lang
F
F
Department for Physiology, University of Tübingen, Germany.
Paulmichl
M
M
D000818:Animals; D002460:Cell Line; D048429:Cell Size; D018118:Chloride Channels / Q000737:chemistry / Q000378:metabolism; D004285:Dogs; D006728:Hormones / Q000494:pharmacology; D007473:Ion Channels / Q000187:drug effects / Q000378:metabolism*; D017136:Ion Transport; D007668:Kidney / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D008564:Membrane Potentials; D015394:Molecular Structure; D015221:Potassium Channels / Q000378:metabolism
D018118:Chloride Channels; D006728:Hormones; D007473:Ion Channels; D015221:Potassium Channels