title: Effect of halothane and isoflurane on in situ diameter responses of small mesenteric veins to acute graded hypercapnia.
abstract: The purpose of the present study was to quantify the inhibitory effect of inhaled halothane and isoflurane on acute hypercapnia-induced responses of capacitance-regulating veins and related cardiovascular variables in response to sequential 40-s periods of 5%, 10%, 15%, and 20% inspired CO2 (FICO2). Measurements were made in normoxic alpha-chloralose-anesthetized rabbits before, during, and after either 0.75 minimum alveolar anesthetic concentration inhaled halothane or isoflurane. The graded hypercapnia caused graded venoconstriction and bradycardia but minimal pressor responses. Hypercapnia-induced venoconstriction was blocked by prior local superfusion of the exposed veins with 3 x 10(-6) M tetrodotoxin. Both the hypercapnia-induced venoconstriction and bradycardia responses were significantly attenuated by halothane or isoflurane and did not fully recover after removal of the anesthetics from the circulation. Both anesthetics produced a significant baseline (i.e., prehypercapnia) hypotension and a tendency toward a resultant tachycardia. The baseline hypotension did not recover completely after elimination of the anesthetic. Neither anesthetic altered baseline vein diameter. These results agree with previous studies demonstrating that hypercapnic acidosis produces mesenteric venoconstriction by elevating excitatory sympathetic efferent neural input via activation of peripheral and central chemoreceptors and that bradycardia results from activation of compensatory baroreflexes. The neural components of these reflexes are possible primary sites for attenuation of these cardiovascular responses by halothane and isoflurane.
authors.affiliation: Department of Anesthesiology, Medical College of Wisconsin, Milwaukee 53226, USA.
authors.forename: T A
authors.initials: TA
authors.lastname: Stekiel
authors.forename: W J
authors.initials: WJ
authors.lastname: Stekiel
authors.forename: M
authors.initials: M
authors.lastname: Tominaga
authors.forename: A
authors.initials: A
authors.lastname: Stadnicka
authors.forename: Z J
authors.initials: ZJ
authors.lastname: Bosnjak
authors.forename: J P
authors.initials: JP
authors.lastname: Kampine
mesh_terms: D000208:Acute Disease; D018685:Anesthetics, Inhalation / Q000494:pharmacology*; D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D001919:Bradycardia / Q000139:chemically induced; D006221:Halothane / Q000494:pharmacology*; D006339:Heart Rate / Q000187:drug effects; D006935:Hypercapnia / Q000503:physiopathology*; D007530:Isoflurane / Q000494:pharmacology*; D008642:Mesenteric Veins / Q000503:physiopathology*; D011817:Rabbits; D013779:Tetrodotoxin / Q000494:pharmacology; D014661:Vasoconstriction / Q000187:drug effects*
chemical_list: D018685:Anesthetics, Inhalation; D013779:Tetrodotoxin; D007530:Isoflurane; D006221:Halothane