[Relation between cell proliferation and beta adrenergic receptor expression in activated T lymphocytes].
beta adrenergic receptors (R) on murine lymphocytes stimulated with concanavalin A (Con A) and tumor lymphoid cell line (BW5147) were characterized by beta radioligand binding of 125Icyanopindolol (125I-CYP). The functionality was also assesses by hormone-dependent changes in cyclic 3', 5', adenosine monophosphate (cAMP) levels on intact cell. Both types of cells displayed a reduced number of beta adrenergic receptors by 125I-CYP specific binding. However BW5147 cell line were unable to respond to beta agonist stimulation meanwhile the response of lymphocytes stimulated with Con A was lower than normal lymphocytes. Despite this difference both kind of cells displayed an increase in cAMP production induced by Prostaglandin E1 (PGE1). It can be concluded that functional beta R are absent in BW5147 cell line. The possible implication of alternative transmission pathway and neuroendocrine control in tumor lymphoid cells is discussed.
Cazaux
C
C
Centro de Estudios Farmacológicos y Botánicos (CEFYBO)-CONICET, Buenos Aires.
Sterin-Borda
L
L
Gorelik
G
G
Cremaschi
G
G
D000818:Animals; D002455:Cell Division / Q000502:physiology; D003208:Concanavalin A / Q000494:pharmacology; D000242:Cyclic AMP / Q000096:biosynthesis*; D066298:In Vitro Techniques; D020120:Iodocyanopindolol; D008213:Lymphocyte Activation; D051379:Mice; D010869:Pindolol / Q000031:analogs & derivatives* / Q000378:metabolism; D011943:Receptors, Adrenergic, beta / Q000096:biosynthesis / Q000502:physiology*; D013601:T-Lymphocytes / Q000166:cytology* / Q000378:metabolism*
D011943:Receptors, Adrenergic, beta; D003208:Concanavalin A; D020120:Iodocyanopindolol; D010869:Pindolol; D000242:Cyclic AMP
Correlación entre la proliferación celular y la expresión de receptores beta adrenérgicos en linfocitos T activados.