Cloning of the mouse gene encoding plasma glutathione peroxidase: organization, sequence and chromosomal localization.
Using a reverse transcription coupled to PCR amplification strategy, with degenerated primers localized in highly conserved domains of known glutathione peroxidase (GPX) proteins, we have generated, from mouse epididymal RNA, a cDNA fragment which was subsequently used to isolate a genomic clone encoding mouse plasma GPX (GPX3). GPX3 is a major enzyme in reducing lipid hydroperoxides and hydrogen peroxide in plasma. We confirm here that the mouse epididymis is a new site of expression of GPX3 and report, together with the sequence, the structural analysis and the chromosomal localization of the mouse GPX3 single-copy gene to chromosome 11.
Schwaab
V
V
Laboratoire de Biologie Cellulaire, CNRS, URA1940, Université Blaise Pascal, Aubière, France.
Baud
E
E
Ghyselinck
N
N
Mattei
M G
MG
Dufaure
J P
JP
Drevet
J R
JR
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D016384:Consensus Sequence; D004822:Epididymis / Q000201:enzymology; D015870:Gene Expression; D005796:Genes; D005979:Glutathione Peroxidase / Q000097:blood / Q000235:genetics*; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics; D012045:Regulatory Sequences, Nucleic Acid; D015183:Restriction Mapping
D012333:RNA, Messenger; D005979:Glutathione Peroxidase