Stabilization of biological specimens by the use of plasma polymerized hydrocarbon film for imaging with an atomic force microscope.
An atomic force microscope makes imaging of biological molecules possible at high resolution. To this end all samples have to be fixed securely to a flat solid support so that they cannot be displaced by the scanning tip. We herein describe a new method to fix the biological samples to glass surfaces by depositing a thin layer of plasma polymerized methane gas in a high vacuum chamber. Such samples can thus be imaged repeatedly by an atomic force microscope without any loss of image quality.
Umeda
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Department of Bacteriology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Amako
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K
Takade
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Tanaka
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A
D017122:Bacteriophage T4 / Q000648:ultrastructure; D016591:Histocytological Preparation Techniques; D006838:Hydrocarbons; D018625:Microscopy, Atomic Force / Q000379:methods*; D011108:Polymers
D006838:Hydrocarbons; D011108:Polymers