The measurement of swimming velocity of Vibrio cholerae and Pseudomonas aeruginosa using the video tracking methods.
The swimming velocities of two monotrichous flagellated bacteria were measured by a computer-assisted video tracking method. Tracing the moving path of the individual bacterium revealed that the bacterial cell did not swim continuously in a straight direction, but frequently changed swimming direction and velocity. The average swimming velocities calculated from the 3-sec path were 75.4 +/- 9.4 microns/sec in four strains of Vibrio cholerae and 51.3 +/- 8.4 microns/sec in five strains of Pseudomonas aeruginosa. These results suggest that V. cholerae swim faster than P. aeruginosa at 30 C in nutrient broth. This method is useful for a detailed analysis of bacterial movement and moving patterns in different environmental conditions.
Shigematsu
M
M
Department of Bacteriology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Meno
Y
Y
Misumi
H
H
Amako
K
K
D009068:Movement; D011550:Pseudomonas aeruginosa / Q000502:physiology*; D014734:Vibrio cholerae / Q000502:physiology*; D014743:Videotape Recording