title: Overexpression of extracellular sucrase (SacC) of Zymomonas mobilis in Escherichia coli.
abstract: The extracellular sucrase (SacC) gene of Zymomonas mobilis was overexpressed in Escherichia coli BL21 using the T7 polymerase expression system. A low cell density induction method was designed to have maximum expression, and the conditions (IPTG concentration, ampicillin addition) were optimised to overexpress to the level of more than 60% of the total cellular protein representing SacC protein.
authors.affiliation: Department of Microbial Technology, School of Biological Sciences, Madurai Kamaraj University, India.
authors.forename: R
authors.initials: R
authors.lastname: Kannan
authors.forename: K
authors.initials: K
authors.lastname: Pitchaimani
authors.forename: P
authors.initials: P
authors.lastname: Gunasekaran
authors.forename: N
authors.initials: N
authors.lastname: Aït-Abdelkader
authors.forename: J
authors.initials: J
authors.lastname: Baratti
mesh_terms: D001431:Bacteriological Techniques; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D015870:Gene Expression / Q000235:genetics; D005798:Genes, Bacterial / Q000235:genetics*; D013393:Sucrase / Q000032:analysis / Q000235:genetics*; D014764:Viral Proteins; D017355:Zymomonas / Q000201:enzymology / Q000235:genetics*
chemical_list: D014764:Viral Proteins; C062210:bacteriophage T7 RNA polymerase; D012321:DNA-Directed RNA Polymerases; D013393:Sucrase