Preparation of characteristic diagram for refolding of lysozyme.
The characteristic diagram for refolding of denatured reduced lysozyme was prepared in terms of recovered activity by employing urea and LiCl concentrations as two axes of rectangular coordinates. The diagram obtained will serve as a new tool not only for the optimum design of refolding media but also for the study of the refolding mechanism.
Tano
K
K
Department of Chemical Reaction Engineering, Faculty of Pharmaceutical Sciences, Nagoya City University, Japan.
Nohara
D
D
Matsubara
M
M
Sakai
T
T
D000818:Animals; D002645:Chickens; D006863:Hydrogen-Ion Concentration; D018021:Lithium Chloride / Q000737:chemistry*; D009113:Muramidase / Q000378:metabolism*; D010084:Oxidation-Reduction; D011489:Protein Denaturation; D017510:Protein Folding; D014508:Urea / Q000737:chemistry*
D014508:Urea; D009113:Muramidase; D018021:Lithium Chloride