The role of the vascular endothelium in the contractile responses of human chorionic plate artery in pre-eclampsia to prostaglandin F2 alpha, 5-hydroxytryptamine, and potassium chloride.
This study characterises the reactivity of chorionic plate artery in pre-eclampsia to prostaglandin F2 alpha (PGF2 alpha), 5-hydroxytryptamine (5-HT), and potassium chloride (KCl) and examines the role of the vascular endothelium in these responses. Ring segments of the chorionic plate arteries of women after normal and pre-eclamptic pregnancies were contracted by PGF2 alpha, 5-HT, and KCl. The experiments were carried out in the presence and absence of endothelium, and on intact rings treated with 10(-6)M indomethacin. The maximal contractile responses of rings from pre-eclamptic women to 5-HT, PGF2 alpha, or KCl were significantly greater than those of rings from normotensive pregnant women. The EC50 values of responses were significantly lower in rings from pre-eclamptic subjects. Endothelium removal and treatment of the rings with indomethacin had no effect on the contractile responses of rings from normotensive pregnant women to all the agents, but significantly increased the EC50 value and decreased the maximal contractile responses of rings from pre-eclamptic women to 5-HT and PGF2 alpha. While de-endothelialisation increased the EC50 value for responses of the rings from pre-eclamptic women to KCl, pretreatment with indomethacin did not significantly affect the KCl-induced responses. The results of the study suggest that pre-eclampsia enhanced the reactivity of human chorionic plate artery to 5-HT, PGF2 alpha, and KCl through the involvement of endothelial derived contracting factors. The increased responses to 5-HT and PGF2 alpha were inhibited by indomethacin, but those to KCl were not.
Ezimokhai
M
M
Department of Obstetrics and Gynaecology, Faculty of Medicine and Health Sciences, United Arab Emirates University, Al Ain, United Arab Emirates.
Aloamaka
C P
CP
Osman
N A
NA
Mensah-Brown
E P
EP
Morrison
J
J
D001158:Arteries / Q000187:drug effects; D002823:Chorion / Q000098:blood supply*; D015237:Dinoprost / Q000627:therapeutic use*; D004305:Dose-Response Relationship, Drug; D004730:Endothelium, Vascular / Q000502:physiology*; D005260:Female; D006801:Humans; D007213:Indomethacin / Q000494:pharmacology; D011189:Potassium Chloride / Q000627:therapeutic use*; D011225:Pre-Eclampsia / Q000188:drug therapy* / Q000503:physiopathology; D011247:Pregnancy; D012701:Serotonin / Q000627:therapeutic use*; D014661:Vasoconstriction / Q000187:drug effects
D012701:Serotonin; D011189:Potassium Chloride; D015237:Dinoprost; D007213:Indomethacin