The potential of oral mucosal cells for cultured epithelium: a preliminary report.
We have developed a method to fabricate cultured epithelium for skin repair using mucosal cells. We grafted this epithelium in six cases. The site where the mucosal epithelium was transplanted keratinized normally within 4 weeks and formed normal skin. Mucosal epithelial cells have many advantages over skin epidermal keratinocytes: (1) Mucosal epithelial cells grow faster than skin keratinocytes. (2) Cultured epithelial sheets formed using mucosal cells remain viable for at least 14 days in vitro. (3) The oral cavity is a suitable location to take a tissue segment because scar due to biopsy is inconspicuous. Therefore, mucosal epithelial cells are a potential new source of cells for cultured epithelial graft.
Ueda
M
M
Department of Oral Surgery, Nagoya University School of Medicine, Japan.
Hata
K
K
Horie
K
K
Torii
S
S
D000328:Adult; D000368:Aged; D018929:Cell Culture Techniques / Q000379:methods; D017690:Cell Transplantation; D004847:Epithelial Cells; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009061:Mouth Mucosa / Q000166:cytology*; D009062:Mouth Neoplasms / Q000601:surgery*; D016038:Skin Transplantation / Q000379:methods