Development and application of an antibody ELISA for the marker protein of ovine pulmonary carcinoma.
Ovine pulmonary carcinoma (OPC) is a contagious pulmonary neoplasia with a suspected retroviral etiology. The major core protein (P27) of the putative OPC virus cross-reacts with antibodies to P27 of the Mason-Pfizer monkey virus (MPMV), a type-D retrovirus. This serological reactivity serves as the only accepted biological marker for OPC. In order to make a useful reagent for the detection of the OPC marker for serodiagnosis and epidemiological studies, the MPMV-P27 coding region was cloned and expressed in Escherichia coli. Gel purified recombinant MPMV-P27 protein was used to develop an immunoassay. This recombinant enzyme-linked immunosorbent assay (ELISA) was then used to screen 223 sera from US sheep and 176 sera from Italian sheep. In this study, we found: (1) a high prevalence of infection with the putative OPC retrovirus in sheep with chronic pneumonia; (2) a subclinical infection with OPC virus may be more common in US sheep than indicated by the rare recorded occurrence of pulmonary carcinoma; (3) an apparent association between ovine lentivirus (OLV) and OPC infection.
Kwang
J
J
USDA, ARS, US Meat Animal Research Center, Clay Center, NE 68933, USA.
Keen
J
J
Rosati
S
S
Tolari
F
F
D002282:Adenocarcinoma, Bronchiolo-Alveolar / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D001483:Base Sequence; D014408:Biomarkers, Tumor; D015153:Blotting, Western; D017931:DNA Primers / Q000737:chemistry; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D004926:Escherichia coli / Q000235:genetics; D015967:Gene Expression Regulation, Viral; D008175:Lung Neoplasms / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D008251:Macaca; D016093:Mason-Pfizer monkey virus / Q000235:genetics / Q000276:immunology; D008969:Molecular Sequence Data; D015995:Prevalence; D011994:Recombinant Proteins / Q000276:immunology; D012192:Retroviridae Infections / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D012680:Sensitivity and Specificity; D012756:Sheep; D012757:Sheep Diseases / Q000175:diagnosis* / Q000821:virology; D014412:Tumor Virus Infections / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D014758:Viral Core Proteins / Q000235:genetics / Q000276:immunology*; D014790:Visna-maedi virus / Q000235:genetics / Q000276:immunology
D000914:Antibodies, Viral; D014408:Biomarkers, Tumor; D017931:DNA Primers; D011994:Recombinant Proteins; D014758:Viral Core Proteins