title: Covalent binding of polycyclic aromatic hydrocarbon components of manufactured gas plant residue to mouse lung and forestomach DNA.
abstract: The present study characterized the DNA adducts induced by manufactured gas plant residue (MGP) and benzo[alpha]pyrene (B[alpha]P) in mouse lung and forestomach. The dose levels used in the present study were comparable to the levels used in a previous animal bioassay. Adduct formation was evaluated in female A/J mice (7 weeks old) fed MGP (0.25%) or B[alpha]P (16 and 98 ppm) for 14 days. In addition, adduct formation was also evaluated in mice 24 h after the ip administration of 1.8 mg of B[alpha]P in 0.5 mL of tricaprylin. 32P-Postlabeling combined with multidimensional TLC and reverse phase HPLC was used to evaluate hydrocarbon-DNA adducts. HPLC separation of chemical-DNA adducts formed in lung following MGP ingestion resulted in three distinct peaks of radioactivity eluting at 22, 32.4, and 33.5 min. These peaks accounted for 13, 10, and 41% of the total adducts detected. The adducts isolated from forestomach eluted as a series of minor peaks with two more distinct peaks of radioactivity at 32.4 and 33.5 min. These peaks accounted for 47 and 32% of the total adducts detected in forestomach, respectively. Ingestion of B[alpha]P (16 or 98 ppm) and the ip administration of B[alpha]P resulted in a single major adduct with a retention time of 32.4 min. The DNA adducts formed from MGP administration were further characterized by comparison with adducts formed following the administration of individual hydrocarbons and a mixture of hydrocarbons.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: College of Pharmacy, Department of Pharmaceutical Chemistry, Rutgers, State University of New Jersey, Piscataway 08855-0789, USA.
authors.forename: E H
authors.initials: EH
authors.lastname: Weyand
authors.forename: Y
authors.initials: Y
authors.lastname: Wu
mesh_terms: D000818:Animals; D002273:Carcinogens / Q000378:metabolism* / Q000633:toxicity; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D003033:Coal Tar / Q000378:metabolism* / Q000633:toxicity; D004247:DNA / Q000378:metabolism; D018736:DNA Adducts / Q000096:biosynthesis* / Q000737:chemistry; D004032:Diet; D005260:Female; D005753:Gastric Mucosa / Q000378:metabolism; D007220:Industrial Waste / Q000009:adverse effects; D008168:Lung / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D008805:Mice, Inbred A; D009153:Mutagens / Q000737:chemistry / Q000633:toxicity; D011084:Polycyclic Aromatic Hydrocarbons / Q000378:metabolism* / Q000633:toxicity; D013270:Stomach / Q000187:drug effects*
chemical_list: D002273:Carcinogens; D018736:DNA Adducts; D007220:Industrial Waste; D009153:Mutagens; D011084:Polycyclic Aromatic Hydrocarbons; D003033:Coal Tar; D004247:DNA