Direct gas chromatographic determination of dechloroethylcyclophosphamide following microsomal incubation of cyclophosphamide.
A method for the sensitive determination of dechloroethylcylclophosphamide (3-DCl) in microsomal incubation mixtures was developed. 3-DCl, a side-chain oxidation product of cyclophosphamide (CP), was isolated by extraction with acetic acid ethyl ester following solid-phase extraction on C8 cartridges. Quantification of the metabolite was performed by direct capillary gas chromatography with a nitrogen-phosphorus detector without prior derivatization. The method showed good sensitivity and reproducibility with a detection limit of 1 ng/ml and a limit of quantification of 5 ng/ml. The suitability of the method is shown for the quantification of 3-DCl following incubation of CP with human liver microsomes.
Bohnenstengel
F
F
Dr. Margarete Fischer-Bosch Institute of Clinical Pharmacology, Stuttgart, Germany.
Johnson
S
S
Hofmann
U
U
Eichelbaum
M
M
Kroemer
H K
HK
D000818:Animals; D000970:Antineoplastic Agents / Q000378:metabolism*; D002197:Capillary Action; D002849:Chromatography, Gas / Q000379:methods* / Q000706:statistics & numerical data; D003520:Cyclophosphamide / Q000031:analogs & derivatives* / Q000032:analysis / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D008862:Microsomes, Liver / Q000378:metabolism*; D051381:Rats; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000970:Antineoplastic Agents; C064445:dechloroethylcyclophosphamide; D003520:Cyclophosphamide