title: Effects of copper-based dental casting alloys on two lymphocyte cell lines and the secretion of interleukin 2 and IgG.
abstract: In the oral environment, gingival lymphocytes are involved in maintaining the local immune defense of periodontal tissues. The corrosion rates of copper-based dental casting alloys and the accumulation of corrosion products in host gingiva raise concerns about the effects of these corrosion products on immune responses in the oral cavity. The aim of this study ws to investigate the hypothesis that immune function may be altered by copper dental alloy corrosion products. In vitro cell culture studies were used to analyze the effects of three copper-based dental alloys on a T-cell and B-cell line and their secretion of soluble immune mediators (IL-2) and effectors (IgG), respectively. Results of this study revealed that corrosion products released from copper alloys in 24 h have the ability to reduce cellular viability, alter proliferation, and modulate the production of soluble immune mediators. These results support the hypothesis that copper dental ally corrosion products may alter immune responses and thereby contribute to a variety of dental pathological conditions.
authors.affiliation: Department of Biomedical Engineering, University of Alabama at Birmingham, USA.
authors.forename: J D
authors.initials: JD
authors.lastname: Bumgardner
authors.forename: L C
authors.initials: LC
authors.lastname: Lucas
authors.forename: M W
authors.initials: MW
authors.lastname: Alverson
authors.forename: A B
authors.initials: AB
authors.lastname: Tilden
mesh_terms: D001402:B-Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D002460:Cell Line; D003300:Copper / Q000633:toxicity*; D003343:Corrosion; D003722:Dental Alloys / Q000633:toxicity*; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis; D007376:Interleukin-2 / Q000096:biosynthesis; D009532:Nickel / Q000494:pharmacology; D018709:Statistics, Nonparametric; D013601:T-Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D013936:Thymidine / Q000378:metabolism; D014025:Titanium / Q000494:pharmacology; D015032:Zinc / Q000494:pharmacology
chemical_list: D003722:Dental Alloys; D007074:Immunoglobulin G; D007376:Interleukin-2; D003300:Copper; D009532:Nickel; D014025:Titanium; D015032:Zinc; D013936:Thymidine