Sodium channels and GABAA receptor-channel complex as targets of environmental toxicants.
Voltage-activated sodium channels and GABAA receptor-chloride channel complex are among the most important target sites of various environmental neurotoxicants. Pyrethroids keep the sodium channels open for prolonged periods of time leading to hyperexcitation of the entire nervous system. In rat cerebellar Purkinje neurons and dorsal root ganglion neurons, only about 1% of sodium channel population needed to be modified by the pyrethroid tetramethrin to increase the depolarizing after-potential to the level of the threshold membrane potential for generation of repetitive after-discharges. This concept of toxicity amplification is applicable to other chemicals that go through a threshold phenomenon to exert their effects. The potency of pyrethroids on neuronal sodium channels increased with lowering the temperature with a Q10 value of 0.2. The selective pyrethroid toxicity between mammals and insects can be quantitatively explained on the basis of the differences in 5 factors, i.e. the intrinsic sodium channel sensitivity, the sodium channel modification due to temperature difference, the reversibility of sodium channel, the detoxication of pyrethroids, and body size. These 5 factors are multiplied to approximately 2000 which is in the same order of magnitude as that of the difference in LD50. Dieldrin had a dual action on the GABAA receptor-chloride channel complex of rat dorsal root ganglion neurons. The initial transient potentiation of GABA-induced currents after application of dieldrin was followed by a suppression. Dieldrin-induced potentiation of current was observed only when the gamma 2 subunit was present in embryonic kidney cells (HEK-293) transfected with GABA receptor subunits. Dieldrin-induced suppression was observed in the presence and absence of the gamma 2 subunit. The dieldrin suppression of GABA-induced currents is deemed directly responsible for hyperactive symptoms of poisoning in animals.
Narahashi
T
T
Department of Molecular Pharmacology and Biological Chemistry, Northwestern University Medical School, Chicago, IL 60611-3008, USA.
Carter
D B
DB
Frey
J
J
Ginsburg
K
K
Hamilton
B J
BJ
Nagata
K
K
Roy
M L
ML
Song
J H
JH
Tatebayashi
H
H
D000818:Animals; D004026:Dieldrin / Q000633:toxicity*; D006801:Humans; D007306:Insecticides / Q000633:toxicity*; D011722:Pyrethrins / Q000633:toxicity*; D051381:Rats; D011963:Receptors, GABA-A / Q000187:drug effects*; D015222:Sodium Channels / Q000187:drug effects*; D013696:Temperature
D007306:Insecticides; D011722:Pyrethrins; D011963:Receptors, GABA-A; D015222:Sodium Channels; D004026:Dieldrin