Alliinase (alliin lyase) from garlic (Alliium sativum) is glycosylated at ASN146 and forms a complex with a garlic mannose-specific lectin.
Alliinase (EC 4.4.1.4) catalyses the production of allicin (thio-2-propene-1-sulfinic acid S-allyl ester), a biologically active compound which is also responsible for the characteristic smell of garlic. It was demonstrated that alliinase which contains 5.5-6% of neutral sugars, gives clear PAS-staining, binds to Con A and can form a complex with garlic mannose-specific lectin (ASA). Evidence that the formation of such a complex is mediated by the interaction of the carbohydrate of the glycoprotein enzyme with the lectin was obtained from a radioligand assay which demonstrated the binding of alliinase to ASA and competitive inhibition of this binding by methyl alpha-D-mannoside. ASA I was shown as the lectin mainly present in the complex with alliinase. The results of this study also demonstrate that alliinase is glycosylated at Asn146 in the sequence Asn146-Met147-Thr148.
Rabinkov
A
A
Department of Membrane Research and Biophysics, Weizmann Institute of Science, Rehovot, Israel.
Wilchek
M
M
Mirelman
D
D
D000595:Amino Acid Sequence; D001216:Asparagine; D013437:Carbon-Sulfur Lyases / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D002918:Chymotrypsin; D003488:Cyanogen Bromide; D004591:Electrophoresis, Polyacrylamide Gel; D005737:Garlic / Q000201:enzymology*; D006020:Glycopeptides / Q000737:chemistry / Q000302:isolation & purification; D006031:Glycosylation; D007700:Kinetics; D037102:Lectins / Q000378:metabolism*; D037241:Mannose-Binding Lectins; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010449:Peptide Mapping; D037121:Plant Lectins; D010946:Plants, Medicinal; D011487:Protein Conformation
D006020:Glycopeptides; D037102:Lectins; D037241:Mannose-Binding Lectins; D037121:Plant Lectins; D001216:Asparagine; D002918:Chymotrypsin; D013437:Carbon-Sulfur Lyases; C019243:alliin lyase; D003488:Cyanogen Bromide