A simple, sensitive, non-stimulated photon counting system for detection of superoxide anion in whole blood.
A simple, sensitive, non-stimulated assay was developed to measure the superoxide anion concentration in whole blood, using an ultra-sensitive chemiluminescence (CL) analyzer and lucigenin amplification. The assay system can be performed without leukocyte isolation or stimulant administration. The blood CL levels of healthy males (362.8 +/- 337.7 counts /10 sec) were not different from those of females (335 +/- 308.7 counts/10 sec) (p=0.64), whereas the CL levels in whole blood in patients with acute pancreatitis (2522 +/- 2014 counts/10 sec) were significantly higher than those of healthy controls (p<0.001). This assay system may be valuable in the future for quantitative measurement of reactive oxygen species in various disorders.
Lu
F J
FJ
Department of Biochemistry, College of Medicine, National Taiwan University, Taipei, Republic of China.
Lin
J T
JT
Wang
H P
HP
Huang
W C
WC
D000166:Acridines; D000208:Acute Disease; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers / Q000097:blood; D005260:Female; D006801:Humans; D008163:Luminescent Measurements; D008297:Male; D008875:Middle Aged; D010195:Pancreatitis / Q000097:blood* / Q000175:diagnosis / Q000503:physiopathology; D017785:Photons; D017382:Reactive Oxygen Species / Q000032:analysis; D012016:Reference Values; D012680:Sensitivity and Specificity; D012727:Sex Characteristics; D012737:Sex Factors; D013481:Superoxides / Q000097:blood*
D000166:Acridines; D015415:Biomarkers; D017382:Reactive Oxygen Species; D013481:Superoxides; C033472:10,10'-dimethyl-9,9'-biacridinium