title: Elevated levels of beta 2 glycoprotein-I (beta 2 GPI) in antiphospholipid antibody syndrome are due to increased amounts of beta 2 GPI in association with other plasma constituents.
abstract: beta 2 glycoprotein-I (beta 2 GPI) is a 50 kDa plasma protein which associates with a number of anionic plasma constituents and has been identified as a cofactor for the binding of some antiphospholipid antibodies (aPAs). beta 2 GPI antigen levels are increased in some patients with aPAs. In order to examine the distribution of beta 2 GPI in these patients, we developed a method for measurement of free beta 2 GPI. Forty-three patients with SLE, of whom 18 had laboratory evidence of aPAs, and 22 normal healthy subjects were studied. Plasma was filtered by centrifugation at 650 x g through a 100 kDa filter in order to allow separation of free and complexed beta 2 GPI, and beta 2 GPI levels were measured in the starting plasma and filtrate by a standardized ELISA. Total beta 2 GPI levels of the SLE aPA positive patients (253.3 mg/l) were significantly increased compared with the SLE aPA negative patients and normal controls (188.0, P < 0.001 and 194.9 mg/l, P < 0.01, respectively), but there were no significant differences between free beta 2 GPI levels of these groups (20.8, 24.0 and 20.5 mg/l, respectively). These results suggest that levels of complexed beta 2 GPI are increased in patients with aPAs, perhaps as a result of immune complex formation, or altered binding to other plasma constituents.
authors.affiliation: Department of Haematology, UCLMS, London, UK.
authors.forename: T
authors.initials: T
authors.lastname: McNally
authors.forename: I J
authors.initials: IJ
authors.lastname: Mackie
authors.forename: S J
authors.initials: SJ
authors.lastname: Machin
authors.forename: D A
authors.initials: DA
authors.lastname: Isenberg
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000936:Antigen-Antibody Complex / Q000097:blood; D016736:Antiphospholipid Syndrome / Q000097:blood* / Q000276:immunology; D001053:Apolipoproteins / Q000097:blood; D001798:Blood Proteins / Q000378:metabolism*; D005260:Female; D006023:Glycoproteins / Q000097:blood*; D006801:Humans; D007123:Immunoelectrophoresis, Two-Dimensional; D008180:Lupus Erythematosus, Systemic / Q000097:blood / Q000276:immunology; D008297:Male; D008875:Middle Aged; D010743:Phospholipids / Q000097:blood; D053482:beta 2-Glycoprotein I
chemical_list: D000936:Antigen-Antibody Complex; D001053:Apolipoproteins; D001798:Blood Proteins; D006023:Glycoproteins; D010743:Phospholipids; D053482:beta 2-Glycoprotein I