Thiophosphate induces apoptosis in human leukemia cell lines.
Thiophosphate induced apoptosis in human HL-60 cells. HL-60 cell proliferation was inhibited with an IC50 of about 60 microM. Typical morphological changes of apoptosis were observed by phase contrast microscopy and DNA laddering was observed after agarose gel electrophoresis. Thiophosphate-induced DNA fragmentation was time and concentration-dependent. After exposure to thiophosphate (100 microM) apoptosis occurred as early as 4 h after treatment and 90% of cells were apoptotic by 24 h. dbcAMP-differentiated HL-60 cells as well as undifferentiated HL-60 cells were susceptible. Thiophosphate was also effective in inducing apoptosis in other leukemia cell lines including CEM and K562 and a lymphoma cell line, Raji.
Jiang
L
L
Department of Biochemistry, University of Sydney, NSW, Australia.
Weston
K M
KM
Conigrave
A D
AD
D017209:Apoptosis / Q000187:drug effects*; D002454:Cell Differentiation; D002455:Cell Division / Q000187:drug effects; D004249:DNA Damage; D004273:DNA, Neoplasm / Q000187:drug effects / Q000302:isolation & purification / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004587:Electrophoresis, Agar Gel; D018922:HL-60 Cells; D006801:Humans; D007700:Kinetics; D010710:Phosphates / Q000494:pharmacology*
D004273:DNA, Neoplasm; D010710:Phosphates; C035638:thiophosphoric acid