A YAC-based contig of 1.5 Mb spanning the human multidrug resistance gene region and delineating the amplification unit in three human multidrug-resistant cell lines.
A contig of 21 nonchimeric yeast artificial chromosomes (YACs) has been assembled across 1.5 Mb of the multidrug resistance (MDR) gene region located at 7q21, and formatted with four previously reported probes, six newly isolated probes, and three sequence-tagged sites (STSs) from internal and end fragments of YACs. A physical map of rare cutter restriction enzyme sites across the region was also constructed by pulsed-field gel electrophoretic (PFGE) analysis of four overlapping YAC clones. The amplification unit of this region in different cell lines was then determined by Southern blot analysis on the basis of the physical map and probes. Amplified DNA was located in extrachromosomal elements in human MDR cell lines studied here, and the size of the amplification unit was determined to be discrete in one MDR amplification but variable in others.
Torigoe
K
K
Department of Biochemistry, Kyushu University School of Medicine, Fukuoka, Japan.
Sato
S
S
Kusaba
H
H
Kohno
K
K
Kuwano
M
M
Okumura
K
K
Green
E D
ED
Tsui
L C
LC
Scherer
S W
SW
Schlessinger
D
D
Wada
M
M
D018435:ATP Binding Cassette Transporter, Subfamily B; D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000235:genetics; D018528:ATP-Binding Cassette Transporters / Q000235:genetics; D001483:Base Sequence; D015139:Blotting, Southern; D002460:Cell Line; D002874:Chromosome Mapping; D018244:Chromosomes, Artificial, Yeast; D002897:Chromosomes, Human, Pair 7 / Q000235:genetics*; D018432:Drug Resistance, Multiple / Q000235:genetics*; D005796:Genes; D005819:Genetic Markers; D006801:Humans; D007624:KB Cells; D008969:Molecular Sequence Data; D016324:Sequence Tagged Sites
D018435:ATP Binding Cassette Transporter, Subfamily B; D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D018528:ATP-Binding Cassette Transporters; D005819:Genetic Markers; C095122:multidrug resistance protein 3