[Expression and localization of immediate early genes and preproenkephalin gene in central nervous system following electroacupuncture stimulation].
Using the techniques of immunohistochemistry, Northern blotting and in situ hybridization, we have found that electroacupuncture (2/15Hz) accelerated the expression of mRNA and protein synthesis of immediate early genes c-fos, c-jun as well as proenkephalin gene in rat central nervous system (CNS). Northern hybridization revealed that EA stimulation induced c-fos gene expression within 1-2 h, followed by PENK gene expression which peaked at 48h. Morphological study demonstrated that the sites of c-fos, c-jun and PENK mRNA expression and protein synthesis coincide with each other in the CNS. The results suggest that Fos/Jun may function as the transcription regulator of PENK gene.
Cui
X
X
Department of Physiology, Beijing Medical University.
D000818:Animals; D001921:Brain / Q000378:metabolism*; D015671:Electroacupuncture; D004745:Enkephalins / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics*; D015870:Gene Expression; D017781:Genes, Immediate-Early; D016762:Genes, fos; D016758:Genes, jun; D011498:Protein Precursors / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics*; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats
D004745:Enkephalins; D011498:Protein Precursors; D012333:RNA, Messenger; C033660:preproenkephalin