Lowered brain glutathione by diethylmaleate decreased the glutamate release induced by cerebral ischemia in anesthetized rats.
The effect of lowered brain glutathione content on the glutamate release following cerebral ischemia was investigated. Diethylmaleate (4 mmol/kg, i.p.), a commonly used chemical reagent for tissue glutathione depletion, significantly reduced the ischemia-induced glutamate release. The release of another excitatory amino acid aspartate was not affected by the diethylmaleate administration. These results suggested that part of the elevated glutamate content induced by ischemia might result from the cellular GSH.
Yang
C S
CS
Department of Medical Research, Taichung Veterans General Hospital, Taiwan, R.O.C.
Lin
N N
NN
Liu
L
L
Tsai
P J
PJ
Kuo
J S
JS
D000777:Anesthetics; D000818:Animals; D001224:Aspartic Acid / Q000378:metabolism; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D002545:Brain Ischemia / Q000378:metabolism*; D018698:Glutamic Acid / Q000378:metabolism*; D005978:Glutathione / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D008298:Maleates / Q000494:pharmacology*; D017551:Microdialysis; D051381:Rats; D017207:Rats, Sprague-Dawley
D000777:Anesthetics; D008298:Maleates; D001224:Aspartic Acid; D018698:Glutamic Acid; C014476:diethyl maleate; D005978:Glutathione