title: Effect of focal cochlear vascular lesion on endocochlear potential in guinea pigs.
abstract: The alteration endocochlear potential (EP) in response to total cochlear ischemia induced by various experimental manipulations has been studied. However, the effect of restricted areal damage to the microvessels (restricted to small area in the lateral wall of a cochlear turn) on the EP value is still unknown. In the current investigation we adopted a photochemical method to produce a focal (i.e., restricted area) microvessel injury in the lateral wall of the guinea pig cochlea and examined the effect of these insults on EP recorded in the same region. The small area of the microvessel lesion (small fenestra: approximately 0.2 x 0.4 mm2) induced by photoactivation did not yield significant EP changes, suggesting that damage to such a small area of microcirculation in the lateral wall of the cochlea has no statistically significant effects on EP values. In subjects with a large area of the microvessel lesion (large fenestra: approximately 0.2 x 0.8 mm2), a decrease in the EP value (mean +/- SEM 7.9 +/- 0.8 mV) was noted. However, the control group animals with a large fenestra but without microvessel lesion also displayed a decrease (8.6 +/- 0.8 mV) in EP. In the current study we were unable to differentiate whether the EP changes in animals with the large fenestra microvessel lesions were caused by the cochlear blood flow decrease or by the surgical preparation. However, the results of this study indicated if the EP value was affected by the large area of the microvessel lesion, the level of decrease would not be large. That is, the EP decrease was less than the EP change in the control group (mean: 8.6 mV). Considering the dependence of EP on blood flow, the data of this study suggest that compensatory mechanisms in the cochlea may maintain the EP following a focal lesion in the lateral wall of the cochlea. This study also indicates that the photochemical method provides a reliable approach to produce the animal model with the focal microvessel lesion in the lateral wall of the cochlea.
authors.affiliation: Institute of Otorhinolaryngology, Chinese Great Wall Hospital, Beijing, China.
authors.forename: B H
authors.initials: BH
authors.lastname: Hu
authors.forename: S C
authors.initials: SC
authors.lastname: Jiang
mesh_terms: D000818:Animals; D003051:Cochlea / Q000098:blood supply* / Q000293:injuries / Q000503:physiopathology; D003055:Cochlear Microphonic Potentials / Q000502:physiology*; D004195:Disease Models, Animal; D006168:Guinea Pigs; D007511:Ischemia / Q000503:physiopathology*; D008833:Microcirculation / Q000473:pathology / Q000503:physiopathology; D010777:Photochemistry