[Electrophoretic analysis on the proteolytic decomposition of of FSH preparations].
We investigated the influence of some proteolytic enzymes being present in the digestive canal (pepsin, trypsin, chymotrypsin) on the digestion of porcine FSH-samples by electrophoresis. Trypsin and Chymotrypsin digested the 18-kDa-protein much faster than pepsin, whereas a 67-kDa-protein was much faster digested by Pepsin than by Trypsin or Chymotrypsin. The overall proteolytic effect of Chymotrypsin is small compared with Pepsin and Trypsin. A subsequent digestion with Pepsin, Trypsin and Chymotrypsin led to a complete proteolysis of all proteins being present in the sample.
Khalaf
H
H
Institut für Medizinische Chemie der Medizinischen Hochschule Hannover.
Neumann
U
U
Rimpler
M
M
D000818:Animals; D002918:Chymotrypsin / Q000378:metabolism*; D004063:Digestion; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005640:Follicle Stimulating Hormone / Q000378:metabolism*; D007700:Kinetics; D010434:Pepsin A / Q000378:metabolism*; D010446:Peptide Fragments / Q000302:isolation & purification; D013552:Swine; D014357:Trypsin / Q000378:metabolism*
D010446:Peptide Fragments; D005640:Follicle Stimulating Hormone; D002918:Chymotrypsin; D014357:Trypsin; D010434:Pepsin A