Assessment of radical activity during the acute phase of myocardial infarction following fibrinolysis: utility of assaying plasma malondialdehyde.
Numerous experimental and clinical studies have reported a role of radical forms of oxygen in the etiology of the manifestations of reperfusion of the ischemic myocardium. However, clinical results remain controversial. The aim of this study was to ascertain the existence of reperfusion-related radical stress after thrombolysis with a marker that is easy to use and reliable. Thirty patients hospitalized for acute myocardial infarction were involved in the study. Of these, 18 had been subjected to intravenous thrombolysis (Group I) and 12 had not (Group II). They were compared to two control groups who had no history of myocardial infarction. Of these, 16 were patients with coronary heart disease hospitalized for stable angina (Group III) and 17 were patients free of any known cardiovascular disease (Group IV). Radical activity was assessed in plasma samples taken from a peripheral vein over a 10-day period of hospitalization by measuring (1) malondialdehydes (MDA) concentrations using fluorometry techniques or HPLC, (2) the antioxidant activity of glutathione peroxidase (GPx) and (3) the concentration of various antiradical compounds (beta-carotene, vitamins A and E, uric acid). All patients in Group I had a patent artery on coronary angiography and showed a significant increase in plasma MDA when compared to those who had not been subjected to thrombolysis (3.15 +/- 0.62 and 2.70 +/- 0.40 mole/l of plasma, respectively). Furthermore, GPx plasma activity was also significantly increased following thrombolysis. By contrast, there was no significant alteration in the antiradical compounds measured. These data suggest that MDA measurements (an early measurement 1-2 days and a late measurement 5-7 days after reperfusion) by fluorometry is a good marker of radical stress during reperfusion in man. The assessment of this marker in patients might represent a simple and reliable test of reperfusion efficacy following thrombolysis, and it might enable one to test the effect of various antioxidant therapies associated with thrombolytic treatment.
Pucheu
S
S
Groupe de Physiopathologie Cellulaire Cardiaque, URA CNRS 1287, Université Joseph Fourier, Grenoble, France.
Coudray
C
C
Vanzetto
G
G
Favier
A
A
Machecourt
J
J
de Leiris
J
J
D000368:Aged; D002338:Carotenoids / Q000097:blood; D002851:Chromatography, High Pressure Liquid; D005260:Female; D005342:Fibrinolysis; D005609:Free Radicals; D005979:Glutathione Peroxidase / Q000097:blood; D006801:Humans; D007700:Kinetics; D008297:Male; D008315:Malondialdehyde / Q000097:blood*; D008875:Middle Aged; D009203:Myocardial Infarction / Q000097:blood* / Q000188:drug therapy; D009218:Myosins / Q000097:blood; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D015912:Thrombolytic Therapy; D014527:Uric Acid / Q000097:blood; D014810:Vitamin E / Q000097:blood; D019207:beta Carotene
D005609:Free Radicals; D017392:Thiobarbituric Acid Reactive Substances; D019207:beta Carotene; D014810:Vitamin E; D014527:Uric Acid; D002338:Carotenoids; D008315:Malondialdehyde; D005979:Glutathione Peroxidase; D009218:Myosins