Modulation of cell death pathways to apoptosis and necrosis of H2O2-treated rat thymocytes by lipocortin I.
Lipocortin I, also called annexin I, a calcium and phospholipid binding protein, protected rat thymocytes from H2O2-elicited necrosis and facilitated H2O2-induced apoptosis, while anti-lipocortin I antibody enhanced H202-elicited necrosis by blocking H202-induced apoptosis. Essentially similar results were obtained with phospholipase A2 inhibitors and activators such as 3,4-octyadecyl-benzylacrylic acid and melittin, respectively. Available evidence suggests that lipocortin I modulates signals for cell death pathways of H2O2-treated rat thymocytes to apoptosis and necrosis by regulating cellular phospholipase A2 activities but not by inhibiting membrane lipid peroxidation.
Sakamoto
T
T
Department of Pharmaceutical Sciences, Wayne State University, Detroit, Michigan 48202, USA.
Repasky
W T
WT
Uchida
K
K
Hirata
A
A
Hirata
F
F
D000818:Animals; D017305:Annexin A1 / Q000276:immunology / Q000494:pharmacology*; D000906:Antibodies / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D016718:Arachidonic Acid / Q000378:metabolism; D016923:Cell Death / Q000187:drug effects*; D002478:Cells, Cultured; D004247:DNA / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D006861:Hydrogen Peroxide / Q000633:toxicity*; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008555:Melitten / Q000494:pharmacology; D009336:Necrosis; D010743:Phospholipids / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013950:Thymus Gland / Q000187:drug effects* / Q000473:pathology / Q000502:physiology
D017305:Annexin A1; D000906:Antibodies; D010743:Phospholipids; D008555:Melitten; D016718:Arachidonic Acid; D004247:DNA; D006861:Hydrogen Peroxide