title: Crypt base cells show forskolin-induced Cl- secretion but no cation inward conductance.
abstract: Whole-cell patch-clamp studies in base cells of isolated colonic crypts of rats pretreated with dexamethasone were performed to examine the effects of stimulation by forskolin (10 micromol/l). The experiments were designed in order to distinguish between two postulated effector mechanisms: the activation of a non-selective cation channel and the activation of Cl- channels. As shown in an accompanying report, forskolin depolarizes the membrane voltage (Vm) by some 40-50 mV and enhances the whole-cell membrane conductance (Gm) substantially in these cells. In this report all experiments were performed in the presence of forskolin. A reduction of the bath Na+ concentration from 145 to 2 mmol/l led to a hyperpolarization of Vm by some 20-30 mV. This hyperpolarization occurred very slowly suggesting that the hyperpolarization produced by the low-Na+ solution was caused indirectly and not by a change in the equilibrium potential for Na+, ENa+. A complete kinetic analysis of the effect on voltage of bath Na+ revealed a saturation-type relation with a high apparent affinity for Na+ of around 5-10 mmol/l. A reduction in bath Cl- concentration from 145 to 32 mmol/l caused a depolarization of Vm from -34 +/- 3 to -20 +/- 4 mV (n = 13) in the presence of a high bath Na+ concentration, but had the opposite effect at low (5 mmol/l) Na+ concentrations: Vm was hyperpolarized from -46 +/- 4 to -62 +/- 6 mV (n = 13). If the effect of Na+ on Vm was caused by a non-selective cation channel the opposite would have been expected. To test directly whether the Na+2Cl-K+ cotransporter was responsible for the effects of changes in bath Na+ on Vm, the effects of increasing concentrations of several loop diuretics were examined. Furosemide, piretanide, torasemide and bumetanide (up to 0.1-0.5 mmol/l) all hyperpolarized Vm, albeit only by less than 10 mV. Another subclass of loop diuretics containing a tetrazolate in position 1 [e.g. azosemide, no. 19A and no. 20A from Schlatter E, Greger R, Weidtke C (1983) Pflüger Arch 396: 210-217] were much more effective. Azosemide hyperpolarized Vm from -46 +/- 3 to -74 +/- 2 mV (n = 18) and reduced Gm from 11 +/- 1 to 4 +/- 1 nS (n = 14). These data indicate that forskolin stimulates Cl- secretion in these cells by a mechanism fully compatible with the current scheme for exocrine secretion involving the Na+2Cl-K+ cotransporter.
authors.affiliation: Physiologisches Institut, Herman Herder Strasse 7, D-79104 Freiburg, Germany.
authors.forename: D
authors.initials: D
authors.lastname: Ecke
authors.forename: M
authors.initials: M
authors.lastname: Bleich
authors.forename: R
authors.initials: R
authors.lastname: Greger
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000502:physiology; D002712:Chlorides / Q000378:metabolism* / Q000494:pharmacology; D005576:Colforsin / Q000494:pharmacology*; D003106:Colon / Q000166:cytology / Q000378:metabolism*; D004231:Diuresis / Q000187:drug effects; D004553:Electric Conductivity; D004594:Electrophysiology; D008536:Meglumine / Q000494:pharmacology; D051381:Rats; D012964:Sodium / Q000502:physiology; D028021:Sodium-Potassium-Chloride Symporters
chemical_list: D002352:Carrier Proteins; D002712:Chlorides; D028021:Sodium-Potassium-Chloride Symporters; D005576:Colforsin; D008536:Meglumine; D012964:Sodium