Analysis of the hemagglutination activity domains of the Venezuelan equine encephalomyelitis and eastern equine encephalomyelitis viruses.
The hemagglutination (HA) domains of the Venezuelan equine encephalomyelitis (VEE) and the eastern equine encephalomyelitis (EEE) viruses providing the interaction of virions and red blood cells were studied with the use of a panel of 17 hemagglutination inhibition (HI) monoclonal antibodies (MAbs). A highly conserved domain (C domain) forming alphavirus-group-reactive MAbs was identified in the E2 protein of the VEE and EEE viruses. These MAbs inhibited HA of the western equine encephalomyelitis, Semliki Forest, Sindbis, Getah, Aura, Chikungunya and Pixuna viruses. The involvement of amino acid residues 59 and 232 in the formation of the C region was demonstrated by sequencing the gene encoding the E2 protein of three escape variants of the VEE virus.
Razumov
I A
IA
Institute of Molecular Biology, State Research Center of Virology and Biotechnology Vector, Koltsovo, Russia.
Khusainova
A D
AD
Agapov
E V
EV
Gaidamovich SYa
Pereboev
A V
AV
Kolykhalov
A A
AA
Netesov
S V
SV
Loktev
V B
VB
D000524:Alphavirus / Q000276:immunology; D000818:Animals; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000940:Antigenic Variation / Q000235:genetics; D001667:Binding, Competitive; D003429:Cross Reactions; D004663:Encephalitis Virus, Eastern Equine / Q000276:immunology*; D004666:Encephalitis Virus, Venezuelan Equine / Q000235:genetics / Q000276:immunology*; D000939:Epitopes / Q000032:analysis*; D005814:Genes, Viral / Q000235:genetics; D006385:Hemagglutination Inhibition Tests; D006387:Hemagglutination, Viral / Q000235:genetics*; D051379:Mice; D017422:Sequence Analysis, DNA; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology*; D015678:Viral Structural Proteins / Q000235:genetics
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000939:Epitopes; D014759:Viral Envelope Proteins; D015678:Viral Structural Proteins; C064956:glycoprotein E2, equine encephalitis virus