Southern blotting analyses of strains of Campylobacter fetus using the conserved region of sapA.
Chromosomal DNA of 27 strains of Campylobacter fetus was analyzed by Southern blotting with a probe of the conserved region of sapA. The probe hybridized with 23 strains that produced type A lipopolysaccharide. These strains had more than six sapA homologs. In Southern blots of SalI-digested chromosomal DNA separated by pulsed-field gel electrophoresis, one fragment from 19 strains and two fragments from 4 strains hybridized. These data indicate that multiple sapA homologs are localized to a limited region on the chromosomal DNA of C. fetus and thus suggest the possibility of developing a typing system using this method.
Fujita
M
M
Department of Bacteriology, Faculty of Medicine, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812, Japan.
Morooka
T
T
Fujimoto
S
S
Moriya
T
T
Amako
K
K
D001426:Bacterial Proteins / Q000235:genetics*; D015373:Bacterial Typing Techniques; D001483:Base Sequence; D015139:Blotting, Southern; D002168:Campylobacter fetus / Q000145:classification / Q000235:genetics*; D017124:Conserved Sequence; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D008562:Membrane Glycoproteins; D008969:Molecular Sequence Data
D001426:Bacterial Proteins; D017931:DNA Primers; D004269:DNA, Bacterial; D008562:Membrane Glycoproteins; C055764:S-layer proteins