Hearing loss and trace elements Fe2+ and Zn2+ in the perilymph.
Fe2+ and Zn2+ levels in perilymph of guinea pigs injected with gentamicin (GM) were examined and compared with the corresponding concentrations in CSF, serum and hairs. We observed a preventive and therapeutic action of sea buckthorn oil and injectio gastrodini to hearing loss. The results showed that: (1) the Fe2+ content in GM-injected guinea pigs was increased in perilymph and hairs; it was decreased after prevention and treatment, but there was no obvious change in CSF and serum; (2) the Zn2+ content in perilymph and CSF was increased in GM-injected guinea pigs. It rose further after prevention, but in serum and hairs it decreased. The results indicate that the ototoxic reaction to GM is related to the rise of the Fe2+ content in perilymph. The elevation of Zn2+ is a compensatory reaction. The changes in the chemical composition of perilymph are more important than those in CSF, serum and hairs for pathological changes of the cochlea. Sea buckthorn oil can prevent GM ototoxicity.
Xu
M
M
Department of Otolaryngology, Second Affiliated Hospital, Xi'an Medical University, China.
Fan
Y
Y
Gao
Z
Z
Chen
J
J
Li
J
J
D000818:Animals; D001300:Audiometry, Evoked Response; D001309:Auditory Threshold / Q000187:drug effects; D001592:Benzyl Alcohols; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use; D005296:Ferrous Compounds / Q000032:analysis / Q000097:blood / Q000134:cerebrospinal fluid; D005839:Gentamicins / Q000009:adverse effects; D005960:Glucosides / Q000627:therapeutic use; D006168:Guinea Pigs; D006197:Hair / Q000737:chemistry; D006311:Hearing Disorders / Q000097:blood / Q000134:cerebrospinal fluid / Q000139:chemically induced / Q000378:metabolism* / Q000517:prevention & control; D007501:Iron / Q000032:analysis* / Q000097:blood / Q000134:cerebrospinal fluid; D010498:Perilymph / Q000737:chemistry*; D010938:Plant Oils / Q000627:therapeutic use; D011500:Protein Synthesis Inhibitors / Q000009:adverse effects; D013054:Spectrophotometry, Atomic; D014131:Trace Elements / Q000032:analysis* / Q000097:blood / Q000134:cerebrospinal fluid; D014801:Vitamin A; D014810:Vitamin E; D014812:Vitamin K; D015032:Zinc / Q000032:analysis* / Q000097:blood / Q000134:cerebrospinal fluid
D001592:Benzyl Alcohols; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D005296:Ferrous Compounds; D005839:Gentamicins; D005960:Glucosides; D010938:Plant Oils; D011500:Protein Synthesis Inhibitors; D014131:Trace Elements; D014801:Vitamin A; D014812:Vitamin K; C059630:vitamin A, vitamin E, vitamin K3 drug combination; D014810:Vitamin E; C045345:gastrodin; D007501:Iron; D015032:Zinc