Influence of strain, biomaterial, proteins, and oncostatic chemotherapy on Staphylococcus epidermidis adhesion to intravascular catheters in vitro.
Initial adhesion of four phenotypically different strains of Staphylococcus epidermidis to 16 silicone, polyurethane, or hydrophilic polyurethane catheters was assessed in vitro by a bacterial radiolabeling method. The effect of catheter exposure to plasma proteins, to an anticancer polychemotherapy (5-fluorouracil, doxorubicin, cyclophosphamide), or to both of them was determined. Bacterial adhesion on native catheters was dependent on the hydrophobicity of both bacteria and catheters. The four strains tested adhered preferentially to silicone catheters (p < 0.05); adhesion was moderate to polyurethane surfaces, whereas the least adhesion was obtained for hydrophilic polyurethane catheters. Adsorption of plasma proteins on the surface produced a marked decrease in adhesion on silicone (-66.2%; p < 0.001) and polyurethane (-32.8%; p < 0.01) catheters and a marked increase in adhesion on hydrophilic surfaces (+91.7%; p < 0.05). Chemotherapeutic treatment of the catheter produced a slight but not significant decrease in adhesion on silicone (-17.4%) and polyurethane (-19.8%) catheters and a marked increase in adhesion on hydrophilic polyurethanes (+148.2%; p < 0.001). The in vitro simulation of catheter use suggested that oncostatic drugs and plasma proteins play an important role in S. epidermidis adhesion to intravascular catheters. Overall, bacterial adhesion is lowest on hydrophilic polyurethane catheters before and after simulation of catheter use.
Galliani
S
S
Clinique des Maladies Infectieuses, Institut Jules Bordet, l'Université Libre de Bruxelles.
Cremieux
A
A
van der Auwera
P
P
Viot
M
M
D000327:Adsorption; D000970:Antineoplastic Agents / Q000494:pharmacology; D001422:Bacterial Adhesion / Q000187:drug effects; D001798:Blood Proteins / Q000502:physiology*; D001808:Blood Vessels; D002404:Catheterization; D003520:Cyclophosphamide / Q000494:pharmacology; D004317:Doxorubicin / Q000494:pharmacology; D004866:Equipment Contamination; D005472:Fluorouracil / Q000494:pharmacology; D006801:Humans; D010641:Phenotype; D011140:Polyurethanes; D012828:Silicones; D013212:Staphylococcus epidermidis / Q000235:genetics / Q000502:physiology*
D000970:Antineoplastic Agents; D001798:Blood Proteins; D011140:Polyurethanes; D012828:Silicones; D004317:Doxorubicin; D003520:Cyclophosphamide; D005472:Fluorouracil