Analysis of the expression and secretion of isotypes of sheep B cell immunoglobulins with a panel of isotype-specific monoclonal antibodies.
Monoclonal antibodies to sheep light chain, IgM and IgG were produced and used to assess total immunoglobulin (Ig) synthesis by sheep B cells in culture and antibodies to specific antigens. By using these antibodies in a dual fluorescence-activated cell sorting analysis of sheep efferent lymph B lymphocytes the percentage change in surface Ig isotype of B lymphoblasts from IgM to IgG after the antigenic stimulation of the local lymph node was measured. An extension of this analysis to paired blood and afferent or efferent lymph B cells made it possible to investigate the recirculation characteristics of B cells expressing different Ig isotypes.
Bird
P
P
Department of Veterinary Pathology, University of Edinburgh, Summerhall.
Jones
P
P
Allen
D
D
Donachie
W
W
Huntley
J
J
McConnell
I
I
Hopkins
J
J
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005434:Flow Cytometry / Q000662:veterinary; D007122:Immunoelectrophoresis / Q000662:veterinary; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000276:immunology*; D007132:Immunoglobulin Isotypes / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D007147:Immunoglobulin Light Chains / Q000096:biosynthesis / Q000276:immunology*; D007075:Immunoglobulin M / Q000096:biosynthesis / Q000276:immunology*; D008198:Lymph Nodes / Q000166:cytology; D012756:Sheep / Q000097:blood / Q000276:immunology*
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D007132:Immunoglobulin Isotypes; D007147:Immunoglobulin Light Chains; D007075:Immunoglobulin M