title: Oligosaccharide sequencing based on exo- and endoglycosidase digestion and liquid chromatographic analysis of the products.
abstract: Exo- and endoglycosidases are used to sequence oligosaccharides and give valuable information on the monosaccharide sequence, together with the anomericity, the stereochemistry, and in some cases, the substitution pattern of the monosaccharides. Both sequential and parallel methods of oligosaccharide sequencing are discussed.
authors.affiliation: Oxford GlycoSystems Ltd., Hitching Court, Abingdon, Oxon, UK.
authors.forename: S
authors.initials: S
authors.lastname: Prime
authors.forename: J
authors.initials: J
authors.lastname: Dearnley
authors.forename: A M
authors.initials: AM
authors.lastname: Ventom
authors.forename: R B
authors.initials: RB
authors.lastname: Parekh
authors.forename: C J
authors.initials: CJ
authors.lastname: Edge
mesh_terms: D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002853:Chromatography, Liquid; D006026:Glycoside Hydrolases / Q000378:metabolism*; D006868:Hydrolysis; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification; D011134:Polysaccharides / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D009844:Oligosaccharides; D011134:Polysaccharides; D006026:Glycoside Hydrolases