Cytokine-mediated human polymorphonuclear neutrophil phagocytosis: evidence of differential sensitivities to manipulation of intracellular mechanisms.
Interleukin-1 beta (IL-1 beta) and tumor necrosis factor alpha (TNF-alpha) are well known mediators in infectious processes with pleiotropic effects on a variety of cells. These cytokines are known to upregulate Fc receptor (Fc gamma R)-mediated phagocytosis by human polymorphonuclear neutrophils (PMN) but the mechanisms of this enhanced phagocytosis are not known. We investigated the effects of various alterations in intra- and extracellular events on cytokine-induced phagocytic upregulation. Blockade of mRNA synthesis, protein kinase C activation, or G protein activation prevented enhanced phagocytosis by either cytokine. The PMN phagocytic response to TNF-alpha, but not IL-1 beta, was also blunted by agents interfering with mRNA translation or Fc gamma R recycling.
Garner
C V
CV
Department of Surgery, Brown University School of Medicine/Rhode Island Hospital, Providence 02903, USA.
D'Amico
R
R
Simms
H H
HH
D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D016207:Cytokines / Q000502:physiology*; D004789:Enzyme Activation; D019204:GTP-Binding Proteins / Q000502:physiology; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology; D007425:Intracellular Membranes / Q000502:physiology; D009504:Neutrophils / Q000502:physiology*; D010587:Phagocytosis / Q000187:drug effects / Q000502:physiology*; D011493:Protein Kinase C / Q000378:metabolism; D012333:RNA, Messenger / Q000037:antagonists & inhibitors; D044087:Receptor, Anaphylatoxin C5a; D011951:Receptors, Complement / Q000378:metabolism; D011961:Receptors, Fc / Q000187:drug effects / Q000378:metabolism; D011994:Recombinant Proteins; D012756:Sheep / Q000097:blood; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D015703:Antigens, CD; D016207:Cytokines; D007375:Interleukin-1; D012333:RNA, Messenger; D044087:Receptor, Anaphylatoxin C5a; D011951:Receptors, Complement; D011961:Receptors, Fc; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D011493:Protein Kinase C; D019204:GTP-Binding Proteins