Opposite regulation of IGF-I and IGF-I receptor mRNA and concomitant changes of GH receptor and IGF-II/M6P receptor mRNA in human IM-9 lymphoblasts.
Human IM-9 lymphoblasts synthesize IGF-I and express IGF-I receptors, IGF-II/M6P receptors and GH receptors. We have studied the regulation of mRNA expression of IGF-I, IGF-I receptors, IGF-II/M6P receptors and GH receptors in IM-9 cells upon serum-withdrawal and re-addition of serum. IM-9 cells were cultured in RPMI-1640 medium with or without serum for various periods of time. RNA was prepared using guanidinium thiocyanate and CsCl. Antisense riboprobes for human IGF-I, IGF-I receptor, IGF-II/M6P receptor, GH receptor and for comparison for human beta-actin were synthesized and labeled with 32P. Protected fragments of 379 bases and of 420 and 350 bases with the IGF-I receptor and with the IGF-I probe respectively and protected fragments of 670 bases and of 51 and 121 bases with the GH receptor and with the beta-actin probe were detected. For the human IGF-II/M6P receptor probe protected fragments of 260 bases were visualized in RNA samples. The amount of mRNA present in each lane (10 microgram total RNA) was determined by computed densitometry. The amount of IGF-mRNA expressed by IM-9 cells decreased rapidly (within two hours) and dramatically (more than 120%) after the withdrawal of serum and increased significantly (220%) after the re-addition of serum. This increase of IGF-I mRNA preceded the increase in cell number that was seen after 48 h of medium change. Conversely, the expression of IGF-I receptor mRNA and beta-actin mRNA increased by more than 250% after the withdrawal of serum within 2 and 8 h respectively, while GH receptor mRNA fell within 2-4 h. The expression of IGF-II/M6P receptor mRNA continued to increase throughout the duration of the cell culture experiment. We conclude that IGF-I and IGF-I receptor mRNAs are regulated in an opposite direction in serum-deprived IM-9 lymphoblasts. In addition, GH receptor mRNA expression parallels IGF-I mRNA expression.
Yang
Y
Y
Children's Hospital, University of München, Munich, Germany.
Hoeflich
A
A
Butenandt
O
O
Kiess
W
W
D000199:Actins / Q000096:biosynthesis; D002455:Cell Division; D002460:Cell Line; D002470:Cell Survival; D016895:Culture Media, Serum-Free; D005786:Gene Expression Regulation; D006801:Humans; D007700:Kinetics; D008214:Lymphocytes; D015345:Oligonucleotide Probes; D016376:Oligonucleotides, Antisense; D010761:Phosphorus Radioisotopes; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis*; D017526:Receptor, IGF Type 1 / Q000096:biosynthesis*; D017527:Receptor, IGF Type 2 / Q000096:biosynthesis*; D011986:Receptors, Somatotropin / Q000096:biosynthesis*; D013997:Time Factors
D000199:Actins; D016895:Culture Media, Serum-Free; D015345:Oligonucleotide Probes; D016376:Oligonucleotides, Antisense; D010761:Phosphorus Radioisotopes; D012333:RNA, Messenger; D017527:Receptor, IGF Type 2; D011986:Receptors, Somatotropin; D017526:Receptor, IGF Type 1