Co-localisation of autoimmune antibodies specific for double stranded DNA with procorticotrophin-releasing hormone within the nucleus of stably transfected CHO-K1 cells.
Human autoantibodies and corticotrophin-releasing hormone (CRH)-specific antibodies have been used in a double-labelling immunofluorescence technique to demonstrate that immunoreactive CRH structures are co-localised with immunostaining produced by double stranded DNA-specific human autoantibodies within the nucleus of cultured ovarian cells of Chinese hamsters (CHO-K1). This co-localisation was confirmed using confocal microscopy. A metabolic labelling technique was used to investigate the role of the cytoskeleton in mediating nuclear translocation of proCRH within stably transfected CHO-K1 cells and showed that microtubule and actin disrupting agents had no effect upon the nuclear translocation of proCRH. These results, therefore, suggest that nuclear translocation of proCRH is not affected by drugs which disrupt the cytoskeleton and, consequently, modify the diameter of the nuclear pores.
Castro
M G
MG
Laboratory of Cellular and Molecular Neurobiology, Department of Physiology, University of Wales College of Cardiff, PO Box 911, Museum Avenue, Cardiff CF1 3US, UK.
Morrison
E
E
Tomasec
P
P
Linton
E A
EA
Lowenstein
P R
PR
D000199:Actins / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D000974:Antibodies, Antinuclear / Q000276:immunology / Q000378:metabolism*; D000963:Antimetabolites / Q000494:pharmacology; D016466:CHO Cells; D002467:Cell Nucleus / Q000276:immunology / Q000378:metabolism; D003346:Corticotropin-Releasing Hormone / Q000378:metabolism*; D006224:Cricetinae; D015638:Cytochalasin D / Q000494:pharmacology; D004247:DNA / Q000276:immunology*; D006801:Humans; D008856:Microscopy, Fluorescence; D008870:Microtubules / Q000187:drug effects / Q000378:metabolism; D015739:Nocodazole / Q000494:pharmacology; D011498:Protein Precursors / Q000378:metabolism; D014162:Transfection
D000199:Actins; D000974:Antibodies, Antinuclear; D000963:Antimetabolites; D011498:Protein Precursors; D015638:Cytochalasin D; D004247:DNA; D003346:Corticotropin-Releasing Hormone; D015739:Nocodazole