title: Structure of the LexA repressor-DNA complex probed by affinity cleavage and affinity photo-cross-linking.
abstract: The structure of the complex of full-length Escherichia coli LexA repressor with a consensus operator DNA fragment has been probed by affinity photo-cross-linking and affinity cleavage. These methods allow the determination of approximate intermolecular distances between a given protein residue and a base or sugar moiety within the operator. In a first step unique cysteine residues were introduced in positions 7, 28, 38, or 52 of the protein. In all four cases, the original amino acid was an arginine. The four amino acids in these positions were expected to be situated on the surface of LexA interacting with DNA, as inferred from the structure of the LexA DNA binding domain [Fogh et al. (1994) EMBO J. 13, 3936-3944]. In a second step, these unique cysteine side chains of the purified proteins were chemically modified either with 4-azidophenacyl bromide or with S-(2-pyridylthio)cysteaminyl-EDTA. The first set of derivatives gives rise to UV-induced cross-linking which may be revealed by alkali/heat treatment; the second leads to direct DNA cleavage in the proximity of the derivatized amino acid. To reduce hydroxyl radical diffusion, the EDTA-iron cleavage reactions were done in the presence of high amounts of glycerol. The results indicate that amino acids 7 and 52 are near nucleotide pairs 8-12 of the operator and that amino acids 28 and 36 of LexA are near nucleotide pairs 5-8 of the operator. The results unambiguously define the orientation of the LexA DNA binding domain relative to the operator and provide support for the model of the LexA-operator complex proposed by Knegtel et al. [(1995) Proteins 21, 226-236]. Ethylation interference experiments further suggest that Arg-7 contacts the phosphate group between nucleotides 8 and 9 as predicted by the model.
authors.affiliation: Institut do Biologie Moléculaire et Cellulaire, Strasbourg, France.
authors.forename: P
authors.initials: P
authors.lastname: Dumoulin
authors.forename: R H
authors.initials: RH
authors.lastname: Ebright
authors.forename: R
authors.initials: R
authors.lastname: Knegtel
authors.forename: R
authors.initials: R
authors.lastname: Kaptein
authors.forename: M
authors.initials: M
authors.lastname: Granger-Schnarr
authors.forename: M
authors.initials: M
authors.lastname: Schnarr
mesh_terms: D000345:Affinity Labels; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D016384:Consensus Sequence; D003432:Cross-Linking Reagents; D004269:DNA, Bacterial / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D004492:Edetic Acid; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D007501:Iron; D008958:Models, Molecular; D008969:Molecular Sequence Data; D015394:Molecular Structure; D016297:Mutagenesis, Site-Directed; D009690:Nucleic Acid Conformation; D009875:Operator Regions, Genetic; D010777:Photochemistry; D011487:Protein Conformation; D012097:Repressor Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D012697:Serine Endopeptidases; D000099160:LexA Repressor Protein
chemical_list: D000345:Affinity Labels; D001426:Bacterial Proteins; D003432:Cross-Linking Reagents; D004269:DNA, Bacterial; D004492:Edetic Acid; D007501:Iron; D012097:Repressor Proteins; D012697:Serine Endopeptidases; D000099160:LexA Repressor Protein