Refined mapping of caltractin in human Xq28 and in the homologous region of the mouse X chromosome places the gene within the bare patches (Bpa) and striated (Str) critical regions.
Caltractin belongs to a family of calcium-binding proteins and is a structural component of the centrosome. A human caltractin cDNA (CALT) has recently been mapped by fluorescence in situ hybridization (FISH) to Xq28. We report here refined mapping of the human CALT gene and its murine homolog between the loci DXS1104 (DXHXS1104) DXS52 (DXHXS52) by PCR and Southern analysis on YACs and somatic cell hybrids from the region in both species. These mapping studies place the gene within the critical region for the murine X-linked dominant, male lethal mutations bare batches and striated.
Chatterjee
A
A
Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA.
Tanaka
T
T
Parrish
J E
JE
Herman
G E
GE
D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002135:Calcium-Binding Proteins / Q000235:genetics*; D002868:Chromosomal Proteins, Non-Histone; D002874:Chromosome Mapping / Q000379:methods*; D018244:Chromosomes, Artificial, Yeast; D006801:Humans; D006822:Hybrid Cells; D051379:Mice; D008969:Molecular Sequence Data; D009154:Mutation; D014960:X Chromosome
D002135:Calcium-Binding Proteins; D002868:Chromosomal Proteins, Non-Histone; C056608:caltractin