title: Patch clamp study of the voltage-dependent anion channel in the thylakoid membrane.
abstract: Measurements of single channel currents were performed on isolated membrane patches from osmotically swollen thylakoids of the Charophyte alga Nitellopsis obtusa. A channel with a high selectivity for anions over cations and a conductance of 100 to 110 pS (114 mm Cl-) was revealed. The channel has a bell-shaped voltage-dependence of the open probability, with a maximum at about 0 mV. This dependence was explained by two gating processes, one causing channel closure at positive and one at negative potentials. The steepness of the voltage-dependence corresponded to approximately 2 elementary charges to be transferred across the entire membrane in each of the two gating processes. The analysis of the anion channel kinetics in the millisecond time domain revealed an e-fold increase of mean open and decrease of mean closed times when the membrane voltage was made more positive by 20 and 36 mV, respectively. Concert transitions of two identical anion channels between open and long inactivated states were observed, while the millisecond closed-open transitions of the two channels within a burst of activity were kinetically independent.
authors.affiliation: Institute for Cell Biophysics, Russian Academy of Sciences, Puschino, Moscow region, Russia.
authors.forename: I I
authors.initials: II
authors.lastname: Pottosin
authors.forename: G
authors.initials: G
authors.lastname: Schönknecht
mesh_terms: D000838:Anions / Q000378:metabolism; D000460:Chlorophyta / Q000378:metabolism*; D002736:Chloroplasts / Q000378:metabolism*; D004553:Electric Conductivity; D007425:Intracellular Membranes / Q000378:metabolism; D015640:Ion Channel Gating; D007473:Ion Channels / Q000378:metabolism*; D018408:Patch-Clamp Techniques
chemical_list: D000838:Anions; D007473:Ion Channels