title: Purification and assay of yeast Sar1p.
authors.affiliation: Genetics Engineering Laboratory, National Food Research Institute, Tsukuba, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Kimura
authors.forename: T
authors.initials: T
authors.lastname: Oka
authors.forename: A
authors.initials: A
authors.lastname: Nakano
mesh_terms: D002850:Chromatography, Gel / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000379:methods; D003001:Cloning, Molecular / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D004721:Endoplasmic Reticulum / Q000378:metabolism; D004926:Escherichia coli; D020558:GTP Phosphohydrolases / Q000302:isolation & purification / Q000378:metabolism; D019204:GTP-Binding Proteins / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D005800:Genes, Fungal; D006056:Golgi Apparatus / Q000378:metabolism; D016244:Guanosine 5'-O-(3-Thiotriphosphate) / Q000378:metabolism; D006153:Guanosine Diphosphate / Q000378:metabolism; D006160:Guanosine Triphosphate / Q000378:metabolism; D007700:Kinetics; D008970:Molecular Weight; D020559:Monomeric GTP-Binding Proteins; D011865:Radioisotope Dilution Technique; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins; D013462:Sulfur Radioisotopes; D033921:Vesicular Transport Proteins
chemical_list: D011994:Recombinant Proteins; D029701:Saccharomyces cerevisiae Proteins; D013462:Sulfur Radioisotopes; D033921:Vesicular Transport Proteins; D006153:Guanosine Diphosphate; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D006160:Guanosine Triphosphate; D020558:GTP Phosphohydrolases; D019204:GTP-Binding Proteins; D020559:Monomeric GTP-Binding Proteins; C077395:SAR1 protein, S cerevisiae