title: Defects in hemostasis in P-selectin-deficient mice.
abstract: Recently, our laboratory showed that platelets, like leukocytes, roll on activated endothelium expressing P-selectin, thus suggesting a role for P-selectin in hemostasis (Frenette et at, Proc Natl Acad Sci USA 92:7450, 1995). We report here that the P-selectin--deficient mice show a 40% prolongation of the bleeding time on amputation of the tip of the tail. Moreover, defective hemostasis was observed in a local Shwartzman-like reaction induced by skin injections of lipopolysaccharide followed by tumor necrosis factor-alpha in the P-selectin--deficient mice. The hemorrhagic lesions, quantitated both macroscopically and microscopically, were twofold larger in the P-selectin--deficient mice. This was also confirmed by measuring the radioactivity in the skin using chromium-labeled red blood cells. Therefore, it is evident that P-selectin plays a role in hemostasis as suggested by its support of platelet rolling.
authors.affiliation: Center for Blood Research, Boston, MA 02115, USA.
authors.forename: M
authors.initials: M
authors.lastname: Subramaniam
authors.forename: P S
authors.initials: PS
authors.lastname: Frenette
authors.forename: S
authors.initials: S
authors.lastname: Saffaripour
authors.forename: R C
authors.initials: RC
authors.lastname: Johnson
authors.forename: R O
authors.initials: RO
authors.lastname: Hynes
authors.forename: D D
authors.initials: DD
authors.lastname: Wagner
mesh_terms: D000818:Animals; D001760:Bleeding Time; D006470:Hemorrhage / Q000503:physiopathology; D006487:Hemostasis; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D019007:P-Selectin / Q000502:physiology*; D012790:Shwartzman Phenomenon
chemical_list: D019007:P-Selectin