Mapping of murine YACs containing the genes Cea2 and Cea4 after B1-PCR amplification and FISH-analysis.
PCR with primers specific for the murine B1 consensus sequence allows amplification of DNA from murine sources. We have used B1-PCR for amplifying yeast artificial chromosome (YAC) DNA which can be used to localize single YACs by fluorescence in situ hybridization. The genes for the pregnancy-specific glycoproteins Cea2 and Cea4, both belonging to the large carcinoembryonic antigen gene family, were localized by chromosomal in situ suppression hybridization of three YAC clones to murine chromosome 7A2-A3. This was facilitated by the use of the mouse lymphoma cell line WMP/WMP which contains nine pairs of Robertsonian fusion chromosomes.
Rettenberger
G
G
Abteilung Medizinische Genetik, Universität Ulm, Germany.
Zimmermann
W
W
Klett
C
C
Zechner
U
U
Hameister
H
H
D000818:Animals; D015139:Blotting, Southern; D002874:Chromosome Mapping; D002875:Chromosomes / Q000648:ultrastructure; D018244:Chromosomes, Artificial, Yeast / Q000235:genetics*; D016384:Consensus Sequence; D004274:DNA, Recombinant / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D006023:Glycoproteins / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D016133:Polymerase Chain Reaction; D011257:Pregnancy Proteins / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid
D004274:DNA, Recombinant; D006023:Glycoproteins; D011257:Pregnancy Proteins