Interleukin 4 (IL-4) and IL-6-producing memory T-cells in peripheral blood and gingival tissue in periodontitis patients with high serum antibody titers to Porphyromonas gingivalis.
We assessed cytokine production and proliferation of memory T-cells that were isolated from peripheral blood of adult periodontitis patients with high anti-Porphyromonas gingivalis titer. Memory T-cells were stimulated with P. gingivalis lipopolysaccharide, sonicates and formalin-killed whole cells. Interleukin 4(IL-4)- and IL-6-producing cells were stained by immunocytochemistry on peripheral blood smears and compared with cryostat sections of autologous gingival biopsies. Memory T-cells in the peripheral blood of patients rated significantly higher than in healthy subjects (32.3 + or - 7.1 vs 25.3 + or - 3.0%). Stimulation of patient-derived memory T-cells with P. gingivalis whole cells induced higher IL-4 production than in healthy subjects (4.4 + or - 4.1% vs 0.7 + or - 0.6%). Induction of IL-4 producing memory T-cells by P. gingivalis lipopolysaccharide and whole cells was respectively 1.37 and 1.56 times that induced by medium alone. IL-6 production did not differ between the groups. Proliferation of memory T-cells in healthy subjects tended to be more inhibited by P. gingivalis antigens than that in patients. In some patients, induction of IL-4- and IL-6-producing memory T-cells in peripheral blood and in autologous gingival biopsies tended to coincide. Memory T-cells with functional characteristics of Th2 could be a crucial cell population capable of reflecting individual susceptibility to periodontitis.
Aoyagi
T
T
Department of Periodontalogy, Niigata University School of Dentistry, Japan.
Sugawara-Aoyagi
M
M
Yamazaki
K
K
Hara
K
K
D000328:Adult; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D002417:Cattle; D005881:Gingiva / Q000276:immunology; D006041:Goats; D006801:Humans; D007156:Immunologic Memory / Q000276:immunology*; D015847:Interleukin-4 / Q000096:biosynthesis*; D015850:Interleukin-6 / Q000096:biosynthesis*; D017493:Leukocyte Common Antigens; D051379:Mice; D008875:Middle Aged; D010518:Periodontitis / Q000276:immunology / Q000382:microbiology*; D016966:Porphyromonas gingivalis / Q000276:immunology*; D011817:Rabbits; D018709:Statistics, Nonparametric; D018418:Th2 Cells / Q000276:immunology / Q000378:metabolism*
D000907:Antibodies, Bacterial; D015850:Interleukin-6; D015847:Interleukin-4; D017493:Leukocyte Common Antigens