Application of intracerebral microdialysis to study regional distribution kinetics of drugs in rat brain.
1. The purpose of the present study was to determine whether intracerebral microdialysis can be used for the assessment of local differences in drug concentrations within the brain. 2. Two transversal microdialysis probes were implanted in parallel into the frontal cortex of male Wistar rats, and used as a local infusion and detection device respectively. Within one rat, three different concentrations of atenolol or acetaminophen were infused in randomized order. By means of the detection probe, concentration-time profiles of the drug in the brain were measured at interprobe distances between 1 and 2 mm. 3. Drug concentrations were found to be dependent on the drug as well as on the interprobe distance. It was found that the outflow concentration from the detection probe decreased with increasing lateral spacing between the probes and this decay was much steeper for acetaminophen than for atenolol. A model was developed which allows estimation of kbp/Deff (transfer coefficient from brain to blood/effective diffusion coefficient in brain extracellular fluid), which was considerably larger for the more lipohilic drug, acetaminophen. In addition, in vivo recovery values for both drugs were determined. 4. The results show that intracerebral microdialysis is able to detect local differences in drug concentrations following infusion into the brain. Furthermore, the potential use of intracerebral microdialysis to obtain pharmacokinetic parameters of drug distribution in brain by means of monitoring local concentrations of drugs in time is demonstrated.
de Lange
E C
EC
Leiden/Amsterdam Center for Drug Research, Division of Pharmacology, University of Leiden, The Netherlands.
Bouw
M R
MR
Mandema
J W
JW
Danhof
M
M
de Boer
A G
AG
Breimer
D D
DD
D000082:Acetaminophen / Q000134:cerebrospinal fluid / Q000737:chemistry / Q000493:pharmacokinetics; D000319:Adrenergic beta-Antagonists / Q000134:cerebrospinal fluid / Q000737:chemistry / Q000493:pharmacokinetics; D018712:Analgesics, Non-Narcotic / Q000134:cerebrospinal fluid / Q000737:chemistry / Q000493:pharmacokinetics; D000818:Animals; D001262:Atenolol / Q000134:cerebrospinal fluid / Q000737:chemistry / Q000493:pharmacokinetics; D001812:Blood-Brain Barrier / Q000502:physiology; D001921:Brain / Q000033:anatomy & histology / Q000378:metabolism*; D003198:Computer Simulation; D004058:Diffusion; D008297:Male; D017551:Microdialysis / Q000379:methods*; D008954:Models, Biological; D010599:Pharmacokinetics; D051381:Rats; D017208:Rats, Wistar; D012044:Regression Analysis; D012984:Software
D000319:Adrenergic beta-Antagonists; D018712:Analgesics, Non-Narcotic; D000082:Acetaminophen; D001262:Atenolol