title: Lower genital tract swabs in the prediction of intrauterine infection in preterm prelabour rupture of the membranes.
abstract: OBJECTIVE
  To examine the genital tract flora in women with preterm prelabour rupture of the membranes and to determine the relationship of these microorganisms to those found in fetal blood and amniotic fluid.
  
  
  METHODS
  Prospective study of 97 patients with preterm prelabour rupture of the membranes.
  
  
  METHODS
  Maternal-fetal medicine centre.
  
  
  METHODS
  High vaginal and endocervical swabs were taken for routine culture of aerobic and anaerobic bacteria and Ureaplasma urealyticum and Mycoplasma hominis. Cordocentesis and amniocentesis were performed and fetal blood and amniotic fluid were cultured for aerobic and anaerobic bacteria. Amniotic fluid was also cultured for Mycoplasma spp. The sensitivities and specificities of genital tract colonisation in the prediction of fetal blood and amniotic fluid infection were calculated.
  
  
  RESULTS
  Positive genital tract cultures for aerobic and anaerobic organisms predicted 40% of positive fetal blood and 53% of positive amniotic fluid cultures with false positive rates of 24% and 25%, respectively. The sensitivity and false positive rates for genital tract colonisation with Mycoplasma spp in the prediction of amniotic fluid infection with these organisms were 85% and 35%, respectively.
  
  
  CONCLUSIONS
  In preterm prelabour rupture of the membranes lower genital tract cultures provide poor prediction of intrauterine infection.
authors.affiliation: Harris Birthright Research Centre for Fetal Medicine, King's College Hospital, London.
authors.forename: S G
authors.initials: SG
authors.lastname: Carroll
authors.forename: S
authors.initials: S
authors.lastname: Papaioannou
authors.forename: I L
authors.initials: IL
authors.lastname: Ntumazah
authors.forename: J
authors.initials: J
authors.lastname: Philpott-Howard
authors.forename: K H
authors.initials: KH
authors.lastname: Nicolaides
mesh_terms: D000653:Amniotic Fluid / Q000382:microbiology; D001424:Bacterial Infections / Q000175:diagnosis*; D002584:Cervix Uteri / Q000382:microbiology*; D005260:Female; D005312:Fetal Blood / Q000382:microbiology; D005322:Premature Rupture of Fetal Membranes / Q000382:microbiology*; D005544:Forecasting; D006801:Humans; D007752:Obstetric Labor, Premature / Q000382:microbiology*; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000175:diagnosis*; D011446:Prospective Studies; D012680:Sensitivity and Specificity; D014621:Vagina / Q000382:microbiology*; D014626:Vaginal Smears