DNA supercoiling changes and nucleoid protein composition in a group of L5178Y cells of varying radiosensitivity.
Cell of the radioresistant L5178Y-R, -S35, -SR and M10(neo 5)-1 and radiosensitive L5178Y-S, M10 and LX830 cell lines were used to investigate the relationship between radiosensitivity and DNA supercoiling ability mediated by the nuclear matrix within chromatin loops containing DNA damage. The ability of DNA loops to undergo changes in supercoiling in the presence of radiation-induced damage revealed that in all cases the degree of inhibition of supercoil rewinding was greater in the radiosensitive cells. Since the amount of DNA damage induced per unit dose is known to be equal in all these cell lines, the same number of DNA lesions produced a greater loss of topological constraint in the radiosensitive cells. The differential loss of DNA supercoiling ability could be due to differences in DNA-nuclear matrix anchor points. High-resolution two-dimensional gel electrophoresis of nucleoid proteins showed numerous reproducible differences in nuclear matrix protein between the cell lines studied. A total of nine proteins were associated with nucleoids from L5178Y-R cells and absent from L5178Y-S nucleoids. None of them, however, correlated absolutely with radioresistance. Thus, unlike previous studies in CHO cells, no candidates for the conveyance of cellular radiosensitivity that were single proteins were detected. However, these results are consistent with the hypothesis that stability of DNA loop domains in the presence of DNA damage is a determinant of the outcome of radiation-induced DNA damage.
Malyapa
R S
RS
Section of Cancer Biology, Washington University, St. Louis, Missouri 63108, USA.
Wright
W D
WD
Roti Roti
J L
JL
D000818:Animals; D002843:Chromatin / Q000528:radiation effects* / Q000648:ultrastructure; D004260:DNA Repair; D004273:DNA, Neoplasm / Q000528:radiation effects*; D004278:DNA, Superhelical / Q000528:radiation effects*; D015180:Electrophoresis, Gel, Two-Dimensional; D007940:Leukemia L5178; D051379:Mice; D009687:Nuclear Proteins / Q000737:chemistry*; D014407:Tumor Cells, Cultured
D002843:Chromatin; D004273:DNA, Neoplasm; D004278:DNA, Superhelical; D009687:Nuclear Proteins