[Alanine dehydrogenase from the cyanobacterium Plectonema boryanum].
Alanine dehydrogenase activity of Plectonema boryanum cells was studied. It was found, that only NAD(H) may be used as the coenzyme. Kinetical parameters for substrates and coenzymes were determined. 2 pH-optima of action have been established for biosynthetic and anabolic reactions. Two isoenzymes of ADG were cleaned using the methods of ion-exchange chromatography and isoelectric focusing. Some physical-chemical properties were studied: pHopt--9-10 and 4.0; temperature optimum -40 degrees C; pI--8.2 and 6.4; molecular weight--50.65 and 52.48 kDa for ADG-1 and ADG-2, respectively. Both enzymes have the subunit structure. Every enzyme is composed by 4 identical polypeptides.
Perepelitsa
S I
SI
Koltukova
N V
NV
Lysenko
T G
TG
D000409:Alanine / Q000737:chemistry*; D050788:Alanine Dehydrogenase; D000594:Amino Acid Oxidoreductases / Q000032:analysis*; D002384:Catalysis; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D000458:Cyanobacteria / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D007527:Isoenzymes / Q000032:analysis; D007700:Kinetics; D008970:Molecular Weight; D009243:NAD / Q000737:chemistry*; D011773:Pyruvates / Q000737:chemistry*; D019289:Pyruvic Acid; D013379:Substrate Specificity; D013696:Temperature
D007527:Isoenzymes; D011773:Pyruvates; D009243:NAD; D019289:Pyruvic Acid; D000594:Amino Acid Oxidoreductases; D050788:Alanine Dehydrogenase; D000409:Alanine