title: Disordered expression of hepatic glycolytic and gluconeogenic enzymes in Otsuka Long-Evans Tokushima fatty rats with spontanteous long-term hyperglycemia.
abstract: Expression of key regulatory enzymes involved in glucose metabolism was studied in the livers of Otsuka Long-Evans Tokushima fatty (OLETF) rats, a model of non-insulin dependent diabetes mellitus. The activity and mRNA levels of glucokinase and L-type pyruvate kinase was increased in the liver of OLETF rats compared with control rats. There was no such remarkable change in liver-type phosphofructokinase. The activities of glucose-6-phosphatase and fructose-1,6-biphosphatase also increase despite high plasma levels of glucose and insulin. The activity of phosphoenolpyruvate carboxykinase did not show any significant change. The mRNA levels for fructose-1,6-biphosphatase, and phosphoenolpyruvate carboxykinase exhibited no marked changes. These results suggest that the expression of glucose-6-phosphatase and fructose-1,6-biphosphatase is disordered in OLETF rats.
authors.affiliation: Second Department of Internal Medicine, Osaka University Medical School, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Hotta
authors.forename: M
authors.initials: M
authors.lastname: Kuwajima
authors.forename: A
authors.initials: A
authors.lastname: Ono
authors.forename: H
authors.initials: H
authors.lastname: Nakajima
authors.forename: R
authors.initials: R
authors.lastname: Shingu
authors.forename: J
authors.initials: J
authors.lastname: Miyagawa
authors.forename: M
authors.initials: M
authors.lastname: Namba
authors.forename: T
authors.initials: T
authors.lastname: Hanafusa
authors.forename: T
authors.initials: T
authors.lastname: Noguchi
authors.forename: N
authors.initials: N
authors.lastname: Kono
authors.forename: Y
authors.initials: Y
authors.lastname: Matsuzawa
mesh_terms: D000818:Animals; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic; D005941:Glucokinase / Q000235:genetics; D005943:Gluconeogenesis; D006019:Glycolysis; D006943:Hyperglycemia / Q000378:metabolism*; D008099:Liver / Q000201:enzymology*; D008297:Male; D010732:Phosphofructokinase-1 / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats
chemical_list: D012333:RNA, Messenger; D010732:Phosphofructokinase-1; D005941:Glucokinase