title: Killing of Schistosoma mansoni sporocysts by Biomphalaria glabrata hemolymph in vitro: alteration of hemocyte behavior after poly-L-lysine treatment of plastic, and the kinetics of killing by different host strains.
abstract: Behavior of hemocytes of the gastropod mollusc Biomphalaria glabrata was markedly changed on plastic by treatment of the substrate with 0.1 mg/ml poly-L-lysine compared to behavior on untreated plastic. On lysine, the cells showed minimal spreading, moved significantly faster, and formed aggregates. Cell-mediated cytotoxicity (CMC) assays were set up on the modified and untreated substrates to compare the killing capacities of B. glabrata hemocytes against Schistosoma mansoni sporocysts. Hemolymph from 1316-R1 (resistant) snails showed higher killing in lysine-treated wells; no significant difference in sporocyst mortality was observed in MO (susceptible) hemolymph between treated and untreated wells. The CMC assays on poly-L-lysine-treated plastic were used to compare the kinetics of parasite killing in hemolymph from 2 susceptible (MO, MRLc) and 2 resistant (1316-R1, 10R2) host strains. Marked differences could be observed between the two resistant snail strains, suggesting different mechanisms of parasite recognition, killing, or both.
authors.affiliation: Department of Zoology, Oregon State University, Corvallis 97331-2914, USA.
authors.forename: A M
authors.initials: AM
authors.lastname: Boehmler
authors.forename: S E
authors.initials: SE
authors.lastname: Fryer
authors.forename: C J
authors.initials: CJ
authors.lastname: Bayne
mesh_terms: D000818:Animals; D001700:Biomphalaria / Q000145:classification / Q000276:immunology*; D002448:Cell Adhesion; D002449:Cell Aggregation; D003602:Cytotoxicity, Immunologic; D006434:Hemocytes / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D006458:Hemolymph / Q000276:immunology*; D007700:Kinetics; D008858:Microscopy, Phase-Contrast; D010969:Plastics; D011107:Polylysine / Q000494:pharmacology*; D012550:Schistosoma mansoni / Q000276:immunology*; D014741:Video Recording
chemical_list: D010969:Plastics; D011107:Polylysine