Isolation and characterization of a pSym locus of Rhizobium sp. BR816 that extends nodulation ability of narrow host range Phaseolus vulgaris symbionts to Leucaena leucocephala.
Introduction of a cosmid library of megaplasmid DNA of Rhizobium sp. BR816, a broad host range Rhizobium strain, into R. etli CE3, a narrow host range bean symbiont, resulted in the isolation of a transconjugant that could effectively nodulate Leucaena leucocephala. Analysis of the corresponding cosmid, pBRF2, revealed the presence of genes required for elicting nitrogen-fixing nodules on L. leucoephala. Subcloning and Tn5 tagging identified a locus responsible for the host range extension. Sequence analysis of this locus revealed an ORF that shows significant identity with NodO of R. leguminosarum bv. viciae.
van Rhijn
P
P
F. A. Janssens Laboratory of Genetics, KU Leuven, Heverlee, Belgium.
Luyten
E
E
Vlassak
K
K
Vanderleyden
J
J
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D002135:Calcium-Binding Proteins; D007887:Fabaceae / Q000382:microbiology*; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D010641:Phenotype; D010946:Plants, Medicinal; D012231:Rhizobium / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D013559:Symbiosis / Q000235:genetics*
D001426:Bacterial Proteins; D002135:Calcium-Binding Proteins; C098296:NodO protein, Leucaena leucocephala; C062095:NodO protein, Rhizobium leguminosarum