In situ hybridization studies of alpha 2-macroglobulin receptor and receptor-associated protein in human prostate carcinoma.
We have utilized in situ hybridization and immunocytochemistry techniques to examine the expression of both alpha 2-macroglobulin (alpha 2-MR) and the 39 kDa receptor-associated protein (RAP) in 8 benign (BPH) and 34 malignant human prostate tissues, including 4 metastases. The levels of alpha 2-MR mRNA expression (but not RAP) increased significantly in high Gleason score carcinomas ( > 8) and in metastatic lesions, suggesting that alpha 2-MR expression is associated with advanced cancer. Semi-quantitative analysis with computer-assisted system analysis (CASA) confirmed this interpretation. This is the first report of alpha 2-MR expression being associated with advanced prostate cancer.
McGarvey
T
T
Department of Pathology, Medical College of Pennsylvania, Philadelphia 19129, USA.
Hussain
M M
MM
Stearns
M E
ME
D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D015342:DNA Probes; D015972:Gene Expression Regulation, Neoplastic; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D026563:LDL-Receptor Related Protein-Associated Protein; D008297:Male; D008969:Molecular Sequence Data; D016376:Oligonucleotides, Antisense / Q000235:genetics; D009843:Oligoribonucleotides / Q000235:genetics; D011470:Prostatic Hyperplasia / Q000378:metabolism*; D011471:Prostatic Neoplasms / Q000737:chemistry* / Q000473:pathology; D012333:RNA, Messenger / Q000032:analysis; D012334:RNA, Neoplasm / Q000032:analysis; D011971:Receptors, Immunologic / Q000032:analysis*; D015854:Up-Regulation; D000511:alpha-Macroglobulins / Q000378:metabolism*
D002352:Carrier Proteins; D015342:DNA Probes; D006023:Glycoproteins; D026563:LDL-Receptor Related Protein-Associated Protein; D016376:Oligonucleotides, Antisense; D009843:Oligoribonucleotides; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D011971:Receptors, Immunologic; D000511:alpha-Macroglobulins