Multiple transcripts of human endothelin-A receptor gene detected by reverse transcription and the polymerase chain reaction.
To elucidate the regulatory mechanism of gene expression of the human endothelin-A receptor (hET-AR), we characterized the hET-AR transcripts using reverse transcription (RT) and polymerase chain reaction (PCR) analysis in a variety of human tissues. The RT-PCR using a set of primers in exons 2 and 5 showed two lower-molecular-weight transcripts in addition to the expected fragment. PCR cloning of these two novel transcripts revealed that these transcripts contain a 199-base pair (bp) and a 327-bp deletion compared with the previously described hET-AR cDNA, respectively. Comparison of their sequences with that of the hET-AR gene showed that the lacking sequences exactly correspond to exon 4 and exons 3 and 4, respectively, suggesting that these lower-molecular-weight ET-AR transcripts may result from alternative RNA splicing. Therefore, we isolated the cDNAs of novel transcripts of hET-AR that might be generated by alternative RNA splicing. These results suggest that the alternative RNA splicing might contribute to the regulation of ET-AR gene expression.
Miyamoto
Y
Y
Department of Medicine and Clinical Science, Kyoto University Graduate School of Medicine, Japan.
Yoshimasa
T
T
Arai
H
H
Takaya
K
K
Ogawa
Y
Y
Itoh
H
H
Nakao
K
K
D001483:Base Sequence; D018076:DNA, Complementary / Q000032:analysis; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis*; D044022:Receptor, Endothelin A; D017466:Receptors, Endothelin / Q000235:genetics*
D018076:DNA, Complementary; D012333:RNA, Messenger; D044022:Receptor, Endothelin A; D017466:Receptors, Endothelin