title: Modulation of K+ channel currents by serum amineoxidase in neurons.
abstract: The influence of bovine serum amineoxidase (SAO), a circulating copper-enzyme, on neuronal K+ channels is described. Bovine SAO enhanced K+ channel currents in N1E-115 neuroblastoma cells in a time-dependent manner. Unlike ceruloplasmin (another copper-protein, shown as depolarizing factor in neurons), SAO had no effect on resting potential of neurons. However, pretreatment of cells with SAO inhibited ceruloplasmin-induced membrane depolarization. Although ceruloplasmin alone inhibited K+ channel currents, it further enhanced K+ channel currents in the presence of SAO. Therefore, SAO may be another endogenous modulator of neuronal K+ channels with effect and mechanisms different from those of ceruloplasmin.
authors.affiliation: Department of Physiology, Université de Montréal, Québec, Canada.
authors.forename: L
authors.initials: L
authors.lastname: Wu
authors.forename: M A
authors.initials: MA
authors.lastname: Mateescu
authors.forename: X T
authors.initials: XT
authors.lastname: Wang
authors.forename: B
authors.initials: B
authors.lastname: Mondovi
authors.forename: R
authors.initials: R
authors.lastname: Wang
mesh_terms: D006631:Amine Oxidase (Copper-Containing); D000818:Animals; D002417:Cattle; D002460:Cell Line; D002570:Ceruloplasmin / Q000494:pharmacology; D007700:Kinetics; D008564:Membrane Potentials / Q000187:drug effects; D008954:Models, Biological; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism*; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000097:blood / Q000494:pharmacology*; D015221:Potassium Channels / Q000187:drug effects* / Q000378:metabolism*
chemical_list: D015221:Potassium Channels; D002570:Ceruloplasmin; D006631:Amine Oxidase (Copper-Containing); D000587:Oxidoreductases Acting on CH-NH Group Donors