title: Proteins functioning in synaptic transmission at the sensory to motor synapse of Aplysia.
abstract: Over expression of Aplysia synaptotagmin in acutely dissected cholinergic neurons from the buccal ganglia, or in primary co-cultures of glutaminergic sensory neurons and motor neurons, causes a reduction synaptic transmission. Anti-sense oligonucleotide treatment of similar cultures produced an enhancement of synaptic transmission. The interaction between Aplysia VAMP/synaptobrevin and syntaxin is reconstructed using the yeast two hybrid system, and used to identify amino acid residues of VAMP/synaptobrevin that are required for this interaction. Point mutations around residue 50, close to the site of cleavage by botulinum toxins specifically disrupt the interaction with syntaxin. An additional VAMP/synaptobrevin binding protein, VAP33, is identified using the yeast two hybrid system. Intracellular injection of VAP33 specific antisera inhibits synaptic transmission in sensory-motor neuron co-cultures.
authors.affiliation: Howard Hughes Medical Institute, College of Physicians and Surgeons of Columbia University, New York, NY 10032, USA.
authors.forename: P A
authors.initials: PA
authors.lastname: Skehel
authors.forename: B A
authors.initials: BA
authors.lastname: Armitage
authors.forename: D
authors.initials: D
authors.lastname: Bartsch
authors.forename: Y
authors.initials: Y
authors.lastname: Hu
authors.forename: B K
authors.initials: BK
authors.lastname: Kaang
authors.forename: S A
authors.initials: SA
authors.lastname: Siegelbaum
authors.forename: E R
authors.initials: ER
authors.lastname: Kandel
authors.forename: K C
authors.initials: KC
authors.lastname: Martin
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001048:Aplysia; D002135:Calcium-Binding Proteins; D002352:Carrier Proteins / Q000032:analysis* / Q000235:genetics; D002478:Cells, Cultured; D008562:Membrane Glycoproteins / Q000032:analysis*; D008565:Membrane Proteins / Q000032:analysis* / Q000235:genetics; D008969:Molecular Sequence Data; D009046:Motor Neurons; D009419:Nerve Tissue Proteins / Q000032:analysis*; D050683:R-SNARE Proteins; D017386:Sequence Homology, Amino Acid; D009435:Synaptic Transmission / Q000502:physiology*; D050857:Synaptotagmins
chemical_list: D002135:Calcium-Binding Proteins; D002352:Carrier Proteins; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D050683:R-SNARE Proteins; D050857:Synaptotagmins