Persistence of 4-nitroquinoline-1-oxide induced lesions in human lymphocytes.
Liquid holding (LH) recovery was matched with three-way differential staining (TWD) to assess the reduction of damage induced in DNA following treatment with 4-nitroquinoline-1-oxide (4NQO) in resting (GO) lymphocytes. Human peripheral lymphocytes (HPL) from three donors were used to evaluate lesion persistence and individual repair capacity. Our data are in contrast to those for diepoxybutane (Ponzanelli et al., 1995) and suggest that LH recovery is completely inefficient in removing 4NQO induced lesions, which are only partially repaired after one cell cycle.
Ponzanelli
I
I
Dipartimento Scienze dell'Ambiente e del Territorio, Università di Pisa, Italy.
Landi
S
S
Barale
R
R
D015112:4-Nitroquinoline-1-oxide / Q000633:toxicity*; D000704:Analysis of Variance; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured / Q000187:drug effects; D004249:DNA Damage; D004260:DNA Repair / Q000502:physiology*; D006801:Humans; D007038:Hypotonic Solutions; D008214:Lymphocytes / Q000187:drug effects / Q000502:physiology; D008940:Mitotic Index; D009153:Mutagens / Q000633:toxicity*; D016192:Resting Phase, Cell Cycle; D012854:Sister Chromatid Exchange; D013194:Staining and Labeling / Q000379:methods
D007038:Hypotonic Solutions; D009153:Mutagens; D015112:4-Nitroquinoline-1-oxide