Electromechanical coupling and the conducted vasomotor response.
Conducted vasomotor responses are viewed as one mechanism that functionally integrates the microvasculature. It is hypothesized that the conducted vasomotor response is the result of an electrical current and its passive electrotonic spread along the length of a microvessel. We tested this hypothesis in isolated, unpressurized arterioles from the hamster cheek pouch using conventional intracellular membrane potential recording techniques. The mean resting membrane potential (RMP) was -67 mV. KCl and phenylephrine (PE) pulse-stimulation applied through micropipettes could both induce transient depolarizations and vasoconstrictions at the site of stimulation (local) and at conducted (560 microns) sites. It was noted, however, that the conducted vasomotor response could not be induced until the conducted electrical response exceeded a threshold of -45 mV for a minimum amount of time. The relationship between the amplitude of constriction and the amplitude-time area of depolarization above -45 mV was the same for local and conducted KCl and for conducted PE but was significantly different from that for local PE. Nifedipine greatly reduced the local and conducted mechanical but not electrical responses. Our results indicate that the conducted vasomotor responses are the result of the generation and subsequent conduction of electrical signals along the vessel but that the corresponding mechanical response occurs only when the electrical response exceeds a threshold level.
Xia
J
J
Department of Molecular Physiology and Biological Physics, School of Medicine, University of Virginia, Charlottesville 22908, USA.
Duling
B R
BR
D000818:Animals; D002610:Cheek / Q000098:blood supply; D006224:Cricetinae; D004553:Electric Conductivity; D004594:Electrophysiology; D066298:In Vitro Techniques; D008297:Male; D008564:Membrane Potentials; D008647:Mesocricetus; D008955:Models, Cardiovascular; D009543:Nifedipine / Q000494:pharmacology; D010656:Phenylephrine / Q000494:pharmacology; D011189:Potassium Chloride / Q000494:pharmacology; D014662:Vasoconstrictor Agents / Q000494:pharmacology; D014665:Vasodilator Agents / Q000494:pharmacology; D014666:Vasomotor System / Q000187:drug effects / Q000502:physiology*
D014662:Vasoconstrictor Agents; D014665:Vasodilator Agents; D010656:Phenylephrine; D011189:Potassium Chloride; D009543:Nifedipine