[Preparation and separation of milligram amounts of canine fibrin(ogen) degradation products (fdp) X, Y, D and E].
In the present investigation we first produced canine fibrinogen degradation products (FDP) following two optimized degradation protocols. These FDP-mixtures, which were alternatively enriched with X and Y fragments or D and E fragments, were purified further to individual FDP X, -Y, -D, and -E with > 95% purity by the means of two low pressure column chromatographic techniques (size exclusion chromatography and anionexchanger chromatography). With this techniques the FDP D could be separated into four different D subfractions. No satisfactory results were yielded by hydrophobic interaction chromatography (HIC) with C5-Alkylsuperose, chromato-focusing and separations with hydroxyapatit. The observed strong binding of fragment E on hydroxyapatit probably points to the maintenance of the calcium binding site on the prepared canine E-fragment.
Wolling
H
H
Klinik für kleine Haustiere, Tierärztliche Hochschule Hannover.
Mischke
R
R
D000818:Animals; D002845:Chromatography; D002850:Chromatography, Gel / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000379:methods; D004285:Dogs; D017886:Durapatite; D005338:Fibrin Fibrinogen Degradation Products / Q000032:analysis / Q000302:isolation & purification*; D012680:Sensitivity and Specificity
D005338:Fibrin Fibrinogen Degradation Products; D017886:Durapatite