title: The fbpABC locus of Neisseria gonorrhoeae functions in the periplasm-to-cytosol transport of iron.
abstract: We have determined that the DNA sequence downstream of the well-characterized gonococcal fbp gene contains two open reading frames: one designated fbpB, which encodes a protein proposed to function as a cytoplasmic permease, and one designated fbpC, which encodes a protein proposed to function as a nucleotide-binding protein. The fpbABC operon composes an iron transport system that is homologous to the sfu and hit operons previously reported for Serratia marcescens and Haemophilus influenzae, respectively, and displays elements characteristic of ATP binding cassette transporters. The fpbABC operon differs from these loci in that it is lethal when overexpressed in Escherichia coli.
authors.affiliation: Department of Molecular Genetics and Biochemistry, University of Pittsburgh School of Medicine, Pittsburg, Pennsylvania 15261, USA.
authors.forename: P
authors.initials: P
authors.lastname: Adhikari
authors.forename: S A
authors.initials: SA
authors.lastname: Berish
authors.forename: A J
authors.initials: AJ
authors.lastname: Nowalk
authors.forename: K L
authors.initials: KL
authors.lastname: Veraldi
authors.forename: S A
authors.initials: SA
authors.lastname: Morse
authors.forename: T A
authors.initials: TA
authors.lastname: Mietzner
mesh_terms: D000595:Amino Acid Sequence; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D001692:Biological Transport; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D027682:Cation Transport Proteins; D003001:Cloning, Molecular; D003593:Cytoplasm / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D004269:DNA, Bacterial; D015964:Gene Expression Regulation, Bacterial; D007501:Iron / Q000378:metabolism*; D033862:Iron-Binding Proteins; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D009344:Neisseria gonorrhoeae / Q000235:genetics / Q000378:metabolism*; D009876:Operon; D033902:Periplasmic Binding Proteins; D017386:Sequence Homology, Amino Acid
chemical_list: D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D002352:Carrier Proteins; D027682:Cation Transport Proteins; D004269:DNA, Bacterial; D033862:Iron-Binding Proteins; D026901:Membrane Transport Proteins; D033902:Periplasmic Binding Proteins; D007501:Iron