title: Influence of soya antigen levels in milk replacers on the disruption of intestinal motility patterns in calves sensitive to soya.
abstract: An experiment was designed to determine the soya antigen levels in milk replacers above which gastrointestinal disorders appeared in preruminant calves previously sensitized to antigenic soya by feeding a soya-based milk replacer for 3 months. These calves were then equipped with wire electrodes on the duodenum and the mid-jejunum. The sensitization was visualized using direct skin testing, plasma anti-soya antibody determination and intestinal myoelectric activity recording. After sensitization, the calves were occasionally fed liquid test meals containing various proportions of antigenic soya and whey. The soya-fed calves displayed larger 24 h skin reactions to beta-conglycinin and higher plasma anti-soya antibody titres than the controls maintained on a skim-milk based milk replacer. Disturbances of the myoelectric activity patterns were recorded on the duodenum and mid-jejunum after feeding antigenic soya, but not non-antigenic soya or milk protein, in the soya-sensitized calves. When the level of antigens was varied, disorders in the jejunal motility patterns appeared when antigenic soya provided one-third or more of the dietary protein in the test meals, although some abnormalities were evident at lower incorporation rates. The major change was a reduction in the mean duration of the jejunal migrating motor complexes which was essentially accounted for by a decrease in the mean duration of the phase I (quiescence). This level of antigens corresponded approximately to 14 and 12 mg of immunoreactive glycinin and beta-conglycinin respectively, per gram of protein intake, i.e. 80 and 70 mg/kg0.75 per test meal.
authors.affiliation: Laboratoire du Jeune Ruminant, Institut National de la Recherche Agronomique-Ecole, Nationale Supérieure Agronomique, Rennes, France.
authors.forename: J P
authors.initials: JP
authors.lastname: Lalles
authors.forename: D
authors.initials: D
authors.lastname: Benkredda
authors.forename: R
authors.initials: R
authors.lastname: Toullec
mesh_terms: D000821:Animal Feed / Q000009:adverse effects / Q000032:analysis*; D000818:Animals; D000941:Antigens / Q000032:analysis* / Q000276:immunology; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology / Q000503:physiopathology*; D005512:Food Hypersensitivity / Q000276:immunology / Q000503:physiopathology / Q000662:veterinary*; D005769:Gastrointestinal Motility / Q000502:physiology*; D005916:Globulins / Q000276:immunology; D008297:Male; D030262:Soybean Proteins; D013025:Glycine max / Q000276:immunology*
chemical_list: D000941:Antigens; D005916:Globulins; D030262:Soybean Proteins; C011474:glycinin