Gas chromatographic determination of cholesterol and tocopherols in edible oils and fats with automatic removal of interfering triglycerides.
An automated gas chromatographic method for the simultaneous determination of cholesterol, alpha-tocopherol and alpha-tocopheryl acetate in edible oils and fats without derivatization is reported. Interferences from lipid material are avoided by using a continuous system to transesterify triglycerides with potassium methylate in methanol. The precision of the method is 1.9, 2.2 and 3.1% for cholesterol, alpha-tocopherol and alpha-tocopheryl acetate, respectively. The proposed methods was validated by analysing a standard reference material of coconut oil (SRM 1563-2) with good results. The method features a high throughput, minimal sample handling and analyte specificity (lipid material does not interfere).
Ballesteros
E
E
Department of Analytical Chemistry, Faculty of Sciences, University of Córdoba, Spain.
Gallego
M
M
Valcárcel
M
M
D002784:Cholesterol / Q000032:analysis*; D002849:Chromatography, Gas / Q000379:methods*; D000074263:Coconut Oil; D004041:Dietary Fats / Q000032:analysis*; D004042:Dietary Fats, Unsaturated / Q000032:analysis*; D007202:Indicators and Reagents; D000432:Methanol / Q000031:analogs & derivatives; D000069463:Olive Oil; D010938:Plant Oils / Q000737:chemistry; D024505:Tocopherols; D014280:Triglycerides / Q000302:isolation & purification*; D014810:Vitamin E / Q000031:analogs & derivatives / Q000032:analysis*; D024502:alpha-Tocopherol / Q000031:analogs & derivatives*
D004041:Dietary Fats; D004042:Dietary Fats, Unsaturated; D007202:Indicators and Reagents; D000069463:Olive Oil; D010938:Plant Oils; D014280:Triglycerides; D014810:Vitamin E; C098207:potassium methylate; D002784:Cholesterol; D024502:alpha-Tocopherol; D000074263:Coconut Oil; D024505:Tocopherols; D000432:Methanol