Quantitative assay of verapamil in drugs and serum by capillary isotachophoresis.
Experimental conditions for a simple capillary isotachophoresis (ITP) with conductometric detection have been created for the determination of verapamil in drugs and serum. The limit of detection (LD) was 80 ng.cm-3 for standard verapamil solution. Under optimal experimental conditions and instrumental comparison, the LD of verapamil in serum was 420 ng.cm-3 without sample pretreatment. To lower the LD of verapamil in serum a liquid-liquid extraction was used. The high recovery (more than 94%) facilitated the verapamil ITP analysis at the therapeutic concentration.
Buzinkaiová
T
T
Department of Analytical Chemistry, Faculty of Chemical Technology, Slovak Technical University, Bratislava, Slovak Republic.
Skacáni
I
I
Netriová
J
J
D002851:Chromatography, High Pressure Liquid; D004586:Electrophoresis; D019075:Electrophoresis, Capillary; D006801:Humans; D013607:Tablets; D014700:Verapamil / Q000032:analysis* / Q000097:blood*
D013607:Tablets; D014700:Verapamil