Expression of c-fms proto-oncogene product by ovarian cancer cell lines with effects of macrophage colony-stimulating factor on proliferation.
We studied the production of macrophage colony-stimulating factor (M-CSF) and the expression of c-fms mRNA, an M-CSF receptor, in four human ovarian cancer cell lines. All four cell lines expressed c-fms mRNA while three secreted M-CSF into the culture medium. The exogenous administration of M-CSF caused no significant enhancement of cellular proliferation in any cell line. Interestingly, the proliferation of KK cells was not affected by anti-M-CSF antibody. These results, taken together with the fact that ovarian cancer cells simultaneously produce M-CSF and c-fms, suggest that an autocrine mechanism may modulate cellular proliferation.
Suzuki
M
M
Department of Obstetrics and Gynecology, Jichi Medical School, School of Medicine, Tochigi, Japan.
Sekiguchi
I
I
Ohwada
M
M
Sato
I
I
Matsui
T
T
Tanabe
T
T
Hashimoto
S
S
Yamada
M
M
D000230:Adenocarcinoma / Q000378:metabolism* / Q000473:pathology; D000888:Antibodies, Anti-Idiotypic / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D005260:Female; D006801:Humans; D016173:Macrophage Colony-Stimulating Factor / Q000096:biosynthesis* / Q000276:immunology / Q000494:pharmacology; D016281:Oncogene Protein gp140(v-fms) / Q000096:biosynthesis*; D010051:Ovarian Neoplasms / Q000378:metabolism* / Q000473:pathology; D000090063:Proto-Oncogene Mas; D012333:RNA, Messenger / Q000096:biosynthesis*; D014407:Tumor Cells, Cultured
D000888:Antibodies, Anti-Idiotypic; C000715470:MAS1 protein, human; D016281:Oncogene Protein gp140(v-fms); D000090063:Proto-Oncogene Mas; D012333:RNA, Messenger; D016173:Macrophage Colony-Stimulating Factor