title: Preferential repair in Saccharomyces cerevisiae rad mutants after induction of interstrand cross-links by 8-methoxypsoralen plus UVA.
abstract: The gene specific induction and the incision step of the removal of 8-methoxypsoralen (8-MOP) plus UVA-induced interstrand cross-links (ICL) was measured in repair mutants of Saccharomyces cerevisiae. Events were examined at the MAT alpha and HML alpha loci in mutants deficient in the repair of ICL, namely rad1, rad2 delta, rad52, pso2 and the rad16 mutant which is impaired in the removal of UV-induced pyrimidine dimers from the silent HML alpha locus. Previously, we observed in a wild-type strain (K107) preferential repair concerning the incision of 8-MOP photo-induced ICL. The present study indicates that the two mutants rad1 and rad2 delta show no repair in either locus, due presumably to their deficiency in the incision step of ICL repair. The rad52 mutant which is defective in recombination, is proficient in the preferential incision of ICL at the MAT alpha locus versus the HML alpha locus. The same is true for the pso2 mutant which also lacks the ability to perform complete repair of ICL. The rad16 mutant is unable to repair ICL in the silent locus HML alpha but is proficient in repair (i.e. the incision of ICL) in the transcriptionally active MAT alpha locus.
authors.affiliation: Institut Curie--Section de Recherche, URA 1292 du CNRS, Paris, France.
authors.forename: V
authors.initials: V
authors.lastname: Meniel
authors.forename: N
authors.initials: N
authors.lastname: Magaña-Schwencke
authors.forename: D
authors.initials: D
authors.lastname: Averbeck
mesh_terms: D000251:Adenosine Triphosphatases; D003432:Cross-Linking Reagents / Q000494:pharmacology; D004260:DNA Repair / Q000187:drug effects*; D045643:DNA Repair Enzymes; D004268:DNA-Binding Proteins / Q000187:drug effects / Q000235:genetics / Q000528:radiation effects; D004706:Endodeoxyribonucleases; D004720:Endonucleases / Q000187:drug effects / Q000235:genetics / Q000528:radiation effects; D005656:Fungal Proteins / Q000187:drug effects / Q000235:genetics* / Q000528:radiation effects; D018398:Homeodomain Proteins; D016023:Integrins / Q000187:drug effects / Q000235:genetics / Q000528:radiation effects; D008730:Methoxsalen / Q000494:pharmacology*; D009687:Nuclear Proteins; D009711:Nucleotides; D017319:Photosensitizing Agents / Q000494:pharmacology*; D051721:Rad52 DNA Repair and Recombination Protein; D012097:Repressor Proteins; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics* / Q000528:radiation effects*; D029701:Saccharomyces cerevisiae Proteins; D015534:Trans-Activators / Q000187:drug effects / Q000235:genetics / Q000528:radiation effects; D014466:Ultraviolet Rays
chemical_list: D003432:Cross-Linking Reagents; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D018398:Homeodomain Proteins; D016023:Integrins; C096063:MATA1 protein, S cerevisiae; D009687:Nuclear Proteins; D009711:Nucleotides; D017319:Photosensitizing Agents; C497197:RAD52 protein, S cerevisiae; D051721:Rad52 DNA Repair and Recombination Protein; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; D015534:Trans-Activators; C070709:integrin alphaEbeta7; D004706:Endodeoxyribonucleases; D004720:Endonucleases; C090162:PSO2 protein, S cerevisiae; C069266:RAD1 protein, S cerevisiae; D000251:Adenosine Triphosphatases; C075467:RAD16 protein, S cerevisiae; D045643:DNA Repair Enzymes; D008730:Methoxsalen