On the mechanism of nitric oxide formation upon oxidative cleavage of C = N(OH) bonds by NO-synthases and cytochromes P450.
Microsomal liver cytochromes P450 catalyze the oxidative cleavage of the C = NOH bond of many ketoximes, amidoximes and guanidoximes, and NO synthases catalyze the oxidation of N omega-hydroxy-L-arginine to citrulline and NO. All these oxidations appear to be performed either by the FE(II) O2 complex of these hemoproteins or by O2.- which is formed by its decomposition. This leads to a unifying view of the mechanisms of P450- and NOS-dependent oxidative cleavage of C = NOH bonds, the relative contribution of Fe(II) O2.- being very different in NO-synthase and cytochromes P450.
Mansuy
D
D
Laboratoire de Chimie et Biochimie Pharmacologiques et Toxicologiques, URA 400 CNRS< Université Paris V, France.
Boucher
J L
JL
Clement
B
B
D000595:Amino Acid Sequence; D000818:Animals; D016384:Consensus Sequence; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009569:Nitric Oxide / Q000096:biosynthesis*; D019001:Nitric Oxide Synthase / Q000378:metabolism*; D010084:Oxidation-Reduction; D010091:Oximes / Q000737:chemistry*
D010091:Oximes; D009569:Nitric Oxide; D003577:Cytochrome P-450 Enzyme System; D019001:Nitric Oxide Synthase