A simple solid-state NMR method for determining peptidoglycan crosslinking in Bacillus subtilis.
The crosslink index of the peptidoglycan of intact cell walls of Bacillus subtilis grown in media containing L-[15N]aspartic acid has been determined by a combination of cross-polarization magic-angle spinning 15N NMR and gas chromatography/mass spectrometry. The crosslink index of 62% decreased to 52% when the bacteria were exposed to the antibiotic cephalothin.
Shenouda
N S
NS
Department of Chemistry, the University of Akron, OH, 44325, USA.
Pan
Y
Y
Schaefer
J
J
Wilson
G E
GE
D001412:Bacillus subtilis / Q000737:chemistry*; D002512:Cephalothin / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D010457:Peptidoglycan / Q000737:chemistry*
D010457:Peptidoglycan; D002512:Cephalothin