Sequence, tissue distribution and developmental changes in rat intestinal oligopeptide transporter.
Complementary DNA clones encoding the rat PepT1 small-intestinal oligopeptide transporter were isolated from a jejunal library by cross-hybridization with a rabbit PepT1 cDNA probe. The cDNA sequence indicates that rat PepT1 is composed of 710 amino acids and shows 77% and 83% amino acid sequence identity with rabbit and human PepT1, respectively. Northern blot analysis detected rat PepT1 mRNA in the small intestine and kidney. Intestinal PepT1 mRNA levels were highest in 4-day old rats, and then decreased reaching the adult level by day 28 after birth. These results indicate that the expressions of PepT1 gene change markedly during development.
Miyamoto
K
K
Department of Clinical Nutrition, School of Medicine, Tokushima University, Japan.
Shiraga
T
T
Morita
K
K
Yamamoto
H
H
Haga
H
H
Taketani
Y
Y
Tamai
I
I
Sai
Y
Y
Tsuji
A
A
Takeda
E
E
D000367:Age Factors; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism*; D018076:DNA, Complementary; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D007421:Intestine, Small / Q000254:growth & development / Q000378:metabolism*; D007583:Jejunum / Q000254:growth & development / Q000378:metabolism; D007668:Kidney / Q000254:growth & development / Q000378:metabolism; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000378:metabolism*; D000074011:Peptide Transporter 1; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011817:Rabbits; D051381:Rats; D017207:Rats, Sprague-Dawley; D017386:Sequence Homology, Amino Acid; D027981:Symporters; D014018:Tissue Distribution
D002352:Carrier Proteins; D018076:DNA, Complementary; D009842:Oligopeptides; D000074011:Peptide Transporter 1; D012333:RNA, Messenger; C082186:SLC15A1 protein, human; C489940:Slc15a1 protein, rat; D027981:Symporters