Biolistic transformation of the obligate plant pathogenic fungus, Erysiphe graminis f.sp. hordei.
Particle gun acceleration appears to be a possible way to transform mycelium cells of obligate plant parasites growing on host surfaces. GUS expression was obtained in E. graminis f.sp. hordei cells after bombardment with the GUS gene under the control of the E. graminis f.sp. hordei &beta;-tubulin promoter. Three heterologous promoters, onefrom Aspergillus nidulans and two from Cochliobolus heterostrophus, gave very low or no expression of GUS.
Christiansen
S K
SK
Plant Genetics Section, Environmental Science and Technology Department, Risø National Laboratory, DK-4000 Roskilde, Denmark.
Knudsen
S
S
Giese
H
H
D001203:Ascomycota / Q000235:genetics*; D001233:Aspergillus nidulans / Q000235:genetics; D005786:Gene Expression Regulation; D005818:Genetic Engineering / Q000379:methods*; D005966:Glucuronidase / Q000096:biosynthesis / Q000235:genetics*; D001467:Hordeum / Q000382:microbiology; D010944:Plants / Q000382:microbiology; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D013997:Time Factors; D014170:Transformation, Genetic; D014404:Tubulin / Q000235:genetics
D011994:Recombinant Proteins; D014404:Tubulin; D005966:Glucuronidase