Transplatin blocks voltage-dependent calcium current in isolated cochlear outer hair cells but is not ototoxic in vivo.
The effects of cisplatin (cis-DDP) and transplatin (trans-DDP) on the voltage-dependent calcium current (ICa) of outer hair cells isolated from the guinea pig cochlea were compared using the whole-cell patch-clamp technique. Both cis-DDP and trans-DDP blocked ICa in a concentration-dependent manner. Trans-DDP did not show ototoxicity or nephrotoxicity in vivo. From these results, it was suggested that the effect of cis-DDP on ICa does not directly lead to cell death.
Saito
T
T
Department of Otolaryngology, Fukai Medical School, Japan.
Yamamoto
T
T
Zhang
Z J
ZJ
Yamada
T
T
Manabe
Y
Y
Saito
H
H
D000818:Animals; D015220:Calcium Channels / Q000187:drug effects* / Q000378:metabolism; D002469:Cell Separation; D002470:Cell Survival / Q000187:drug effects; D002945:Cisplatin / Q000494:pharmacology*; D004553:Electric Conductivity; D006168:Guinea Pigs; D018072:Hair Cells, Auditory, Outer / Q000187:drug effects* / Q000378:metabolism*; D018408:Patch-Clamp Techniques
D015220:Calcium Channels; C034697:transplatin; D002945:Cisplatin