Cloning and characterization of a cDNA encoding a precursor for human uroguanylin.
Uroguanylin, a member of the guanylin peptide family, is an endogenous activator of intestinal guanylate cyclase (GC-C). A cDNA encoding a precursor for human uroguanylin was cloned from a human colon cDNA library and sequenced. The precursor was 112 amino acids long and included a signal peptide at the N-terminus and the human uroguanylin sequence at the C-terminus. RNA blot analysis and the reverse transcription-polymerase chain reaction (RT-PCR) showed that human uroguanylin mRNA is expressed in the stomach and intestine. Uroguanylin, as well as guanylin, may be a potent physiological regulator of intestinal fluid and electrolyte transport.
Miyazato
M
M
Third Department of Medicine, Miyazaki Medical College, Kiyotake, Japan.
Nakazato
M
M
Yamaguchi
H
H
Date
Y
Y
Kojima
M
M
Kangawa
K
K
Matsuo
H
H
Matsukura
S
S
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers; D018076:DNA, Complementary; D005768:Gastrointestinal Hormones; D015870:Gene Expression; D015723:Gene Library; D006801:Humans; D008969:Molecular Sequence Data; D045265:Natriuretic Peptides; D016376:Oligonucleotides, Antisense; D010452:Peptide Biosynthesis; D010455:Peptides; D016133:Polymerase Chain Reaction; D011498:Protein Precursors / Q000096:biosynthesis*; D021382:Protein Sorting Signals / Q000096:biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis; D017386:Sequence Homology, Amino Acid
D017931:DNA Primers; D018076:DNA, Complementary; D005768:Gastrointestinal Hormones; D045265:Natriuretic Peptides; D016376:Oligonucleotides, Antisense; D010455:Peptides; D011498:Protein Precursors; D021382:Protein Sorting Signals; D012333:RNA, Messenger; C083435:preproguanylin; C072685:guanylin; C084362:uroguanylin