Peptide attachment to extremities of liposomal surface grafted PEG chains: preparation of the long-circulating form of laminin pentapeptide, YIGSR.
Poly(ethylene glycol) (PEG)-grafted liposomes offer new opportunities as long-circulating platforms presenting biologically relevant ligands. In pursuit of this goal, liposomal conjugates of YIGSR were prepared by mild periodate oxidation of TYIGSR-NH2 and incubation of the product with hydrazide-PEG-(distearoylphosphatidyl)ethanolamine-containing liposomes. The peptide-carrying liposomes, with up to 500 YIGSR residues per vesicle, despite exhibiting faster blood clearance rates than the parent liposomes in rats, remained in circulation for extended periods of time. Mean residence times for the parent liposomal formulation and conjugated preparations containing 200 and 500 YIGSR residues per vesicle were 28, 25, and 23 h, respectively. The results have important implications for systemic delivery of peptides and for their use as targeting moieties for PEG-grafted liposomes.
Zalipsky
S
S
SEQUUS Pharmaceuticals, Inc., Menlo Park, California 94025, USA.
Puntambekar
B
B
Boulikas
P
P
Engbers
C M
CM
Woodle
M C
MC
D000595:Amino Acid Sequence; D000818:Animals; D004337:Drug Carriers; D007797:Laminin / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry*; D008297:Male; D008657:Metabolic Clearance Rate; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000737:chemistry* / Q000493:pharmacokinetics; D010714:Phosphatidylethanolamines / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014018:Tissue Distribution
D004337:Drug Carriers; D007797:Laminin; D008081:Liposomes; D009842:Oligopeptides; D010714:Phosphatidylethanolamines; C038089:1,2-distearoylphosphatidylethanolamine; D011092:Polyethylene Glycols