[Mechanisms of the early effect of biologically active hydroxysterols: calcitriol and ecdysterone. Identification of sphingomyelin as the effector mechanism of the early effect].
Variations of sphingomyelin metabolism in the tissue of brain, liver, heart and mucosa of small intestine of healthy rats were studied in the in vivo experiments using L-[methyl-3H]methionine for 1h after administration of 10(-8)-10(-12) M of biologically active hydroxysterols--steroid hormones: ecdysterone (20-hydroxyecdysone) and calcitriol (1 alpha, 25-dihydroxy vitamin D3). Considerable activation (5-10 times) of sphingomyelin hydrolysis is observed 1-2 min after peroral administration of hydroxysterols. An essential increase of the pools of sphingosin is observed. Sphingosin is a modulator of activity of protein kinase C taking part in the transfer of the external signal to the cell. It is supposed that sphingomyelin metabolism modulation can be one of the effector mechanisms of early (pregenom) action of steroid hormones--ecdysterone and calcitriol.
Kotsiuruba
A B
AB
Tuhanova
A V
AV
Bukhanevich
O M
OM
Tarakanov
S S
SS
D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D002117:Calcitriol / Q000494:pharmacology*; D004441:Ecdysterone / Q000494:pharmacology*; D006321:Heart / Q000187:drug effects; D006868:Hydrolysis; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013109:Sphingomyelins / Q000378:metabolism*; D013997:Time Factors
D013109:Sphingomyelins; D004441:Ecdysterone; D002117:Calcitriol