The role of vitamin E in the susceptibility of rat lung and liver microsomes to iron-stimulated peroxidation.
The production of thiobarbituric acid-reactive substances (TBA-RS) and ethane, two markers of the lipid peroxidation process, was evaluated in rat lung and liver microsomal membranes incubated in the presence of either ferrous ions or a mixture of ferric ions and ascorbate. Microsomal fractions isolated from lung tissue were more resistant than those isolated from the liver. Compared to Fe2+, the association of Fe3+/ascorbate seemed to be totally ineffective in stimulating peroxidation of lung microsomes. The fatty acid profile of lung and liver microsomal membranes could not be responsible for their different susceptibility to free radical degradation. The microsomal fraction isolated from lung showed a higher vitamin E concentration than the liver. The importance of vitamin E in protecting lung membranes was assessed by using lung and liver isolated from vitamin E-deficient and vitamin E-supplemented rats. For both lung and liver microsomal fractions an inverse relationship between vitamin E concentrations and the extent of lipid peroxidation was observed. However, although the vitamin E concentrations in lung and liver microsomes isolated from rats submitted to a vitamin E-deficient diet were not different, lung microsomes still exhibited a lower production of TBA-RS and ethane than liver. In addition to vitamin E, other factors must be involved to explain the resistance of lung microsomes to lipid peroxidation.
Schweich
M D
MD
Industrial Toxicology and Occupational Medicine Unit, School of Medicine, Catholic University of Louvain, Brussels, Belgium.
Lison
D
D
Lauwerys
R
R
D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology; D004980:Ethane / Q000032:analysis / Q000378:metabolism; D005227:Fatty Acids / Q000032:analysis / Q000378:metabolism; D007501:Iron / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects* / Q000502:physiology; D008168:Lung / Q000648:ultrastructure*; D008297:Male; D008861:Microsomes / Q000737:chemistry / Q000378:metabolism / Q000502:physiology*; D008862:Microsomes, Liver / Q000737:chemistry / Q000378:metabolism / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis / Q000378:metabolism; D014810:Vitamin E / Q000378:metabolism / Q000502:physiology*
D005227:Fatty Acids; D017392:Thiobarbituric Acid Reactive Substances; D014810:Vitamin E; D007501:Iron; D004980:Ethane; D001205:Ascorbic Acid