Movement of cortical actin patches in yeast.
In yeast, actin forms patches associated with the plasma membrane. Patch distribution correlates with polarized growth during the cell cycle and in response to external stimuli. Using green fluorescent protein fused to capping protein to image actin patches in living cells, we find that patches move rapidly and over long distances. Even patches in clusters, such as at the incipient bud site, show movement. Patches move independently of one another and generally over small distances in a local area, but they can also move larger distances, including through the mother-bud neck. Changes in patch polarization occur quickly through the cell cycle. These observations provide important new parameters for a molecular analysis of the regulation and function of actin.
Waddle
J A
JA
Department of Genetics, Washington University School of Medicine, St. Louis, Missouri, 63110, USA.
Karpova
T S
TS
Waterston
R H
RH
Cooper
J A
JA
D000199:Actins / Q000378:metabolism*; D001692:Biological Transport; D002352:Carrier Proteins; D002451:Cell Compartmentation; D002453:Cell Cycle; D002462:Cell Membrane / Q000378:metabolism*; D016764:Cell Polarity; D003598:Cytoskeletal Proteins; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins; D008565:Membrane Proteins; D008840:Microfilament Proteins; D008856:Microscopy, Fluorescence; D009068:Movement; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; D013997:Time Factors; D015003:Yeasts / Q000254:growth & development / Q000378:metabolism*
D000199:Actins; C090135:Cap2 protein, S cerevisiae; D002352:Carrier Proteins; D003598:Cytoskeletal Proteins; D008164:Luminescent Proteins; D008565:Membrane Proteins; D008840:Microfilament Proteins; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; D049452:Green Fluorescent Proteins