Determination of barnidipine in human serum and dog plasma by HPLC with electrochemical detection.
Barnidipine is a 1,4-dihydropyridine calcium antagonist. HPLC was conducted on a polybutadiene coated alumina column using an alkaline mobile phase and an electrochemical detector to determine the content of this drug in serum and plasma. A good linear relationship between barnidipine concentration and peak height was found in 5-500 ng/ml with a correlation coefficient of 0.998. The detection limit was 1 ng/ml. The within-day and day-to-day variations were examined for control human serum. Relative standard deviation of within-day assay for serum spiked with 10 ng/ml barnidipine.HCl was 6.9% and the recovery was 104%. A pharmacokinetic study was made in which the time course of barnidipine in dog plasma was followed.
Takamura
K
K
School of Pharmacy, Tokyo University of Pharmacy and Life Science, Japan.
Abdel-Wadood
H M
HM
Kusu
F
F
Rafaat
I H
IH
Saleh
G A
GA
el-Rabbat
N A
NA
Otagiri
M
M
D000818:Animals; D002121:Calcium Channel Blockers / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid; D004285:Dogs; D004563:Electrochemistry; D006801:Humans; D007202:Indicators and Reagents; D009543:Nifedipine / Q000031:analogs & derivatives* / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics; D010777:Photochemistry
D002121:Calcium Channel Blockers; D007202:Indicators and Reagents; C050699:mepirodipine; D009543:Nifedipine