title: Endogenous ubiquinol prevents protein modification accompanying lipid peroxidation in beef heart submitochondrial particles.
abstract: This article is a study of the relationship between lipid peroxidation and protein modification in beef heart submitochondrial particles, and the protective effect of endogenous ubiquinol (reduced coenzyme Q) against these effects. ADP-Fe3+ and ascorbate were used to initiate lipid peroxidation and protein modification, which were monitored by measuring TBARS and protein carbonylation, respectively. Endogenous ubiquinone was reduced by the addition of succinate and antimycin. The parameters investigated included extraction and reincorporation of ubiquinone, and comparison of the effect of ubiquinol with those of various antioxidant compounds and enzymes, as well as the iron chelator EDTA. Under all conditions employed there was a close correlation between lipid peroxidation and protein carbonylation, and the inhibition of these effects by endogenous ubiquinol. SDS-PAGE analysis revealed a differential effect on individual protein components and its prevention by ubiquinol. Conceivable mechanisms behind the observed oxidative modifications of membrane phospholipids and proteins and of the role of ubiquinol in preventing these effects are considered.
authors.affiliation: Division for Medical Cell Biology, Clinical Research Centre at NOVUM, Karolinska Institute, Huddinge, Sweden.
authors.forename: P
authors.initials: P
authors.lastname: Forsmark-Andrée
authors.forename: G
authors.initials: G
authors.lastname: Dallner
authors.forename: L
authors.initials: L
authors.lastname: Ernster
mesh_terms: D000818:Animals; D000968:Antimycin A / Q000031:analogs & derivatives / Q000494:pharmacology; D000975:Antioxidants / Q000494:pharmacology; D001205:Ascorbic Acid / Q000494:pharmacology; D002417:Cattle; D004492:Edetic Acid / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D007700:Kinetics; D015227:Lipid Peroxidation; D008929:Mitochondria, Heart / Q000648:ultrastructure*; D011506:Proteins / Q000378:metabolism*; D013367:Submitochondrial Particles / Q000378:metabolism*; D013386:Succinates / Q000494:pharmacology; D019802:Succinic Acid; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D014451:Ubiquinone / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000502:physiology
chemical_list: D000975:Antioxidants; D011506:Proteins; D013386:Succinates; D017392:Thiobarbituric Acid Reactive Substances; C032456:antimycin; D014451:Ubiquinone; D000968:Antimycin A; D004492:Edetic Acid; D019802:Succinic Acid; C003741:ubiquinol; D001205:Ascorbic Acid