Temporal expression of ECE-1, ET-1, ET-3, ETA, and ETB receptor mRNAs after balloon angioplasty in the rat.
The temporal expressions of endothelin-converting enzyme-1 (ECE-1), preproendothelin-1 (preproET-1), preproET-3, and ETA, ETB receptor mRNAs were studied in rat carotid artery after balloon angioplasty using quantitative reverse transcription and the polymerase chain reaction (RT-PCR). The expression profile of these messages revealed that the increases of ECE-1 mRNA preceded those of preproET-1 and preproET-3. A significant increase in ECE-1 mRNA was observed at 6 h (1.8-fold, p < 0.01) and 24 h (1.7-fold, p < 0.01) after angioplasty, whereas a significant increase in preproET-1 mRNA levels was not observed until day 3 (1.9-fold, p < 0.05) and day 7 (2.1-fold, p < 0.05). A similar delayed increase in preproET-3 mRNA was observed at day 7 (2.8-fold, p < 0.05) and day 14 (2.6-fold, p < 0.05) after angioplasty. The levels of ETA receptor mRNA were elevated 29.3-fold (p < 0.001) and 24.3-fold (p < 0.01) at days 3 and 7 after angioplasty, respectively. The increase in ETB receptor mRNA occurred slightly earlier than that of ETA receptor, showing a 15.1-fold increase at day 1 (p < 0.001) and a 11.3-fold increase at day 3 (p < 0.01) after angioplasty. The data suggest that ECE-1, ET-1, ET-3, and ETA and ETB receptors play an active role in the pathogenesis of neointima formation.
Wang
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Department of Cardiovascular Pharmacology, SmithKline Beecham Pharmaceuticals, King of Prussia, Pennsylvania 19406, USA.
Douglas
S A
SA
Feuerstein
G Z
GZ
Ohlstein
E H
EH
D000800:Angioplasty, Balloon; D000818:Animals; D016282:Aspartic Acid Endopeptidases / Q000235:genetics*; D001483:Base Sequence; D000072396:Endothelin-Converting Enzymes; D016232:Endothelins / Q000235:genetics*; D008297:Male; D008666:Metalloendopeptidases; D008969:Molecular Sequence Data; D009131:Muscle, Smooth, Vascular / Q000473:pathology; D012333:RNA, Messenger / Q000032:analysis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D044022:Receptor, Endothelin A; D044023:Receptor, Endothelin B; D017466:Receptors, Endothelin / Q000235:genetics*
D016232:Endothelins; D012333:RNA, Messenger; D044022:Receptor, Endothelin A; D044023:Receptor, Endothelin B; D017466:Receptors, Endothelin; D016282:Aspartic Acid Endopeptidases; D008666:Metalloendopeptidases; D000072396:Endothelin-Converting Enzymes