Vigilin contains a functional nuclear localisation sequence and is present in both the cytoplasm and the nucleus.
Vigilin is a member of the KH protein family and contains 14 tandemly arranged potential RNA-binding domains. Between KH domains 2 and 3 we have identified a nuclear localization sequence by cloning this sequence into the NH2-terminal region of phage T7 RNA polymerase as a reporter protein and by showing its transfer into the nucleus. Furthermore we provide experimental evidence that Vigilin is present both in the nucleus and in the cytoplasm in similar concentrations. These observations support the notion that Vigilin may shuttle between nucleus and cytoplasm presumably in contact with RNA molecules.
Kügler
S
S
Institut fur Medizinische Molekularbiologie, Medizinische Universität zu Lübeck, Lübeck, Germany.
Grünweller
A
A
Probst
C
C
Klinger
M
M
Müller
P K
PK
Kruse
C
C
D000595:Amino Acid Sequence; D000818:Animals; D017123:Bacteriophage T7 / Q000201:enzymology; D002352:Carrier Proteins; D002460:Cell Line; D002467:Cell Nucleus / Q000737:chemistry*; D002645:Chickens; D003001:Cloning, Molecular; D003593:Cytoplasm / Q000737:chemistry*; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D017930:Genes, Reporter / Q000235:genetics; D008099:Liver / Q000737:chemistry; D008969:Molecular Sequence Data; D017433:Protein Structure, Secondary; D011506:Proteins / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D016601:RNA-Binding Proteins; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000032:analysis; D014764:Viral Proteins
D002352:Carrier Proteins; D011506:Proteins; D016601:RNA-Binding Proteins; D011993:Recombinant Fusion Proteins; D014764:Viral Proteins; C075197:high density lipoprotein binding protein; C062210:bacteriophage T7 RNA polymerase; D012321:DNA-Directed RNA Polymerases