DNA fingerprinting of Brassica juncea cultivars using microsatellite probes.
The genetic variability in the Brassica juncea cultivars was detected by employing in-gel hybridization of restricted DNA to simple repetitive sequences such as (GATA)4, (GACA)4 and (CAC)5. The most informative probe/enzyme combination was (GATA)4/EcoRI, yielding highly polymorphic fingerprint patterns for the B. juncea cultivars. This technique was found to be dependable for establishing the variety specific patterns for most of the cultivars studied, a prerequisite for germplasm preservation. The results of the present study were compared with those reported in our earlier study in which random amplification of polymorphic DNA (RAPD) was used for assessing the genetic variability in the B. juncea cultivars.
Bhatia
S
S
Biotechnology Division, Tata Energy Research Institute, New Delhi, India,
Das
S
S
Jain
A
A
Lakshmikumaran
M
M
D001483:Base Sequence; D001937:Brassica / Q000235:genetics*; D016172:DNA Fingerprinting; D018744:DNA, Plant / Q000235:genetics*; D018895:Microsatellite Repeats; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes; D019105:Random Amplified Polymorphic DNA Technique
D018744:DNA, Plant; D015345:Oligonucleotide Probes