Effects of bipolar point and line stimulation in anisotropic rabbit epicardium: assessment of the critical radius of curvature for longitudinal block.
Excitation front shape and velocity were studied in anisotropic perfused rabbit epicardium stained with potentiometric fluorescent dye. In the combined results from all experiments, convex excitation fronts produced by stimulation with a single electrode propagated longitudinally 13.3% slower than flat excitation fronts produced by stimulation with a line of electrodes. For transverse propagation, the two stimulation methods produced similar flat excitation fronts and velocities. The critical excitation front radius of curvature for longitudinal block (Rcr), calculated from excitable media theory, was 92 microns in control hearts. In hearts exposed to diacetyl monoxime (20 mmol/L), which decreases inward sodium current, Rcr was 175 microns. The slower longitudinal propagation velocity of convex fronts versus flat fronts and the theoretically predicted critical radius of curvature may be important for propagation and block of ectopic depolarizations in the heart.
Knisley
S B
SB
Division of Cardiovascular Disease, School of Medicine, University of Alabama, Birmingham 35294-0019, USA.
Hill
B C
BC
D000200:Action Potentials / Q000187:drug effects / Q000502:physiology; D000818:Animals; D016880:Anisotropy; D003931:Diacetyl / Q000031:analogs & derivatives / Q000494:pharmacology; D004558:Electric Stimulation / Q000295:instrumentation / Q000379:methods; D004566:Electrodes; D005456:Fluorescent Dyes; D006327:Heart Block / Q000503:physiopathology; D066298:In Vitro Techniques; D010496:Pericardium / Q000187:drug effects / Q000502:physiology*; D011817:Rabbits
D005456:Fluorescent Dyes; C004717:diacetylmonoxime; D003931:Diacetyl