EF-1 alpha is a target site for an inhibitory effect of quercetin in the peptide elongation process.
The effect of quercetin (3,3',4',5,7-pentahydroxyflavone) on the polypeptide elongation system isolated from rat liver cells, was investigated. Quercetin inhibited [14C]leucine incorporation into proteins in vitro and the inhibitory effect is being directed towards the elongation factor eEF-1, but not to eEF-2 and ribosomes. Quercetin was found to form a complex with EF-1 alpha, which was inactive in GTP-dependent binding to ribosomes. It can be suggested that quercetin can block the total or the part of the domain of EF-1 alpha structure that is responsible for formation of the ternary complex EF-1 alpha-GTP-[14C]Phe-tRNA and therefore preventing formation of the quaternary complex with ribosomes.
Marcinkiewicz
C
C
Department of General and Organic Chemistry, Medical Academy, Białystok, Poland.
Gałasiński
W
W
Gindzieński
A
A
D000818:Animals; D006160:Guanosine Triphosphate / Q000378:metabolism; D066298:In Vitro Techniques; D007930:Leucine / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D010441:Peptide Chain Elongation, Translational / Q000187:drug effects* / Q000502:physiology; D020648:Peptide Elongation Factor 1; D010445:Peptide Elongation Factors / Q000037:antagonists & inhibitors* / Q000378:metabolism; D011794:Quercetin / Q000494:pharmacology*; D012360:RNA, Transfer, Phe / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012270:Ribosomes / Q000187:drug effects / Q000378:metabolism
D020648:Peptide Elongation Factor 1; D010445:Peptide Elongation Factors; D012360:RNA, Transfer, Phe; D006160:Guanosine Triphosphate; D011794:Quercetin; D007930:Leucine