title: Effects of aluminum adjuvant on systemic reactions of lipopolysaccharides in swine.
abstract: In vivo effects of aluminum adjuvant on systemic reaction of bacterial lipopolysaccharide (LPS) in piglets were investigated. Intramuscular injection of 0.1 mg kg-1 of LPS added to aluminum hydroxide gel (LPS(+)AL) mitigated the leukopenia, trembling and serum levels of TNF-alpha and cortisol compared with the injection of LPS suspended in LPS-free saline (LPS(+)SALINE). The serum endotoxin levels were reduced remarkably but relatively long-lasting in the LPS(+)AL. The lethality in mice injected with LPS added to aluminum hydroxide gel was significantly reduced. Likewise, the Limulus activity of a test LPS was reduced by the addition of aluminum hydroxide gel or aluminum chloride.
authors.affiliation: National Veterinary Assay Laboratory, Tokyo, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Norimatsu
authors.forename: Y
authors.initials: Y
authors.lastname: Ogikubo
authors.forename: A
authors.initials: A
authors.lastname: Aoki
authors.forename: T
authors.initials: T
authors.lastname: Takahashi
authors.forename: G
authors.initials: G
authors.lastname: Watanabe
authors.forename: K
authors.initials: K
authors.lastname: Taya
authors.forename: S
authors.initials: S
authors.lastname: Sasamoto
authors.forename: M
authors.initials: M
authors.lastname: Tsuchiya
authors.forename: Y
authors.initials: Y
authors.lastname: Tamura
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000536:Aluminum Hydroxide / Q000494:pharmacology*; D000818:Animals; D004347:Drug Interactions; D004731:Endotoxins / Q000097:blood; D004926:Escherichia coli; D006854:Hydrocortisone / Q000097:blood; D008033:Limulus Test; D008070:Lipopolysaccharides / Q000633:toxicity*; D051379:Mice; D008815:Mice, Inbred Strains; D013552:Swine; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D000276:Adjuvants, Immunologic; D004731:Endotoxins; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D000536:Aluminum Hydroxide; D006854:Hydrocortisone