Mitochondrial bioenergetics as affected by cationic detergents.
This paper examines the accumulation and toxicity mechanism of a cationic detergent, cetyltrimethylammonium (bromide) (CTAB), in energized rat liver mitochondria. The results suggest that: (1) the CTAB ion is accumulated in the mitochondrial matrix by a membrane potential-driven uptake mechanism; (2) accumulation may lead to a toxic effect, since it gives rise to collapse of the mitochondrial membrane potential (delta phi) which is correlated with ATP synthesis and which regulates Ca++ uptake; (3) collapse of delta phi may be due to enhanced permeability of the mitochondrial membrane to the ions (detergent effect); and (4) delta phi collapse and Ca++ and K+ release were also observed in another cationic detergent, NTAB, but not in the presence of anionic detergents.
Bragadin
M
M
Department of Environmental Sciences, University of Venice, Italy.
Dell'Antone
P
P
D000255:Adenosine Triphosphate / Q000096:biosynthesis; D000818:Animals; D002118:Calcium / Q000378:metabolism; D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000378:metabolism / Q000633:toxicity*; D003902:Detergents / Q000633:toxicity*; D004734:Energy Metabolism / Q000187:drug effects*; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects; D008930:Mitochondria, Liver / Q000187:drug effects* / Q000378:metabolism; D011188:Potassium / Q000378:metabolism; D000644:Quaternary Ammonium Compounds / Q000378:metabolism / Q000633:toxicity; D051381:Rats; D017208:Rats, Wistar
D002593:Cetrimonium Compounds; D003902:Detergents; D000644:Quaternary Ammonium Compounds; C098347:nonyltrimethylammonium; D000255:Adenosine Triphosphate; D011188:Potassium; D002118:Calcium; D000077286:Cetrimonium