Proteins that interact with GTP in Streptomyces griseus and its possible implication in morphogenesis.
By cross-linking with [alpha-32P]GTP or [gamma-32P]GTP with or without UV treatment, several proteins of Streptomyces griseus were shown to interact with GTP in specific ways. After gel electrophoresis, 19 bands of radioactivity were found; 12 bands were assigned as GTP-binding proteins and 6 bands as phosphorylated proteins. One band was assumed to be a guanylylated protein. The profile of radioactive bands was similar between cells prepared from liquid or solid culture, but markedly different between growth phases. A mutant (strain M-1) defective in aerial mycelium formation, which was originally found as a decoyinine-resistant isolate, was found to have a different profile of phosphorylated proteins.
Itoh
M
M
National Food Research Institute, Ibaraki, Japan.
Penyige
A
A
Okamoto
S
S
Ochi
K
K
D001426:Bacterial Proteins / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D019204:GTP-Binding Proteins / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D008970:Molecular Weight; D009024:Morphogenesis; D010766:Phosphorylation; D013302:Streptomyces / Q000254:growth & development; D013305:Streptomyces griseus / Q000737:chemistry / Q000254:growth & development*
D001426:Bacterial Proteins; D019204:GTP-Binding Proteins