Evaluation of lung function after intratracheal perfluorocarbon administration in healthy animals.
OBJECTIVE
To investigate the effects of partial liquid ventilation (i.e., mechanical ventilation in combination with intratracheal administration of perfluorocarbon) on lung function, with particular attention to the integrity of the alveolocapillary membrane in healthy adult animals.


METHODS
Prospective, randomized, controlled study.


METHODS
Laboratory at the Department of Experimental Anesthesiology, Erasmus University Rotterdam.


METHODS
Ten adult male New Zealand rabbits.


METHODS
Five rabbits were intratracheally treated with 12 mL/kg of perfluorocarbon while conventional mechanical ventilation (volume-controlled, tidal volume of 12 mL/kg, respiratory rate of 30 breaths/min, inspiration/expiration ratio of 1:2, positive end-expiratory pressure of 2 cm H2O, and an FIO2 of 1.0) was applied for 3 hrs. To assess the permeability of the alveolocapillary membrane, pulmonary clearance of inhaled technetium-99m-labeled diethylenetriamine pentaacetic acid (99mTc-DTPA) measurements were performed at 3 hrs and compared with data from the control group (n = 5) treated with mechanical ventilation only, using the same ventilatory parameters.


RESULTS
Pulmonary gas exchange and lung mechanical parameters were measured in both groups at 30-min intervals. Mean values for PaO2 in the perfluorocarbon group, although at adequate levels, were less than those values of the control group during the 3-hr study period (370 +/- 44 vs. 503 +/- 44 torr at 3 hrs [49.3 +/- 5.9 vs. 67.1 +/- 5.9 kPa]). Peak and mean airway pressures were higher in the perfluorocarbon group (ranging from 1.9 to 3.4 cm H2O and 0.7 to 1.3 cm H2O, respectively) compared with the control group, while end-inspiratory airway pressure was similar in both groups. The half-life of 99mTc-DTPA was 83.7 +/- 24.5 mins in the control group, which was significantly longer (p < .01) than in the perfluorocarbon group (49.8 +/- 6.1 mins).


CONCLUSIONS
These findings suggest that partial liquid ventilation with perfluorocarbons lowers pulmonary gas exchange in healthy animals, and the increased pulmonary clearance of 99mTc-DTPA after 3 hrs of this type of ventilatory support may reflect minimal reversible changes in the lung surfactant system.
Tütüncü
A S
AS
Department of Anesthesiology, Erasmus University Rotterdam, The Netherlands.
Houmes
R J
RJ
Bos
J A
JA
Wollmer
P
P
Lachmann
B
B
D000818:Animals; D002199:Capillary Permeability / Q000187:drug effects; D004353:Drug Evaluation, Preclinical; D005466:Fluorocarbons / Q000494:pharmacology*; D007322:Instillation, Drug; D008297:Male; D008657:Metabolic Clearance Rate; D011650:Pulmonary Alveoli / Q000098:blood supply / Q000187:drug effects; D011659:Pulmonary Gas Exchange / Q000187:drug effects*; D011817:Rabbits; D011897:Random Allocation; D012121:Respiration, Artificial / Q000379:methods*; D015656:Respiratory Mechanics / Q000187:drug effects*; D016284:Technetium Tc 99m Pentetate / Q000493:pharmacokinetics
D005466:Fluorocarbons; D016284:Technetium Tc 99m Pentetate