title: Reutilization of previously hybridized slides for fluorescence in situ hybridization.
abstract: Application of fluorescence in situ hybridization (FISH) to clinical material is sometimes limited by sample size. In addition, heterogeneity among slides prepared from a single sample may lead to variation in FISH analyses. Reutilization of material for repeated FISH analyses would help to alleviate these problems. We have developed a simple procedure for repeated FISH analyses with directly conjugated probes. Previously hybridized probes are removed by incubation in denaturing solution, and slides can then be rehybridized without residual signals remaining. Several cycles of this procedure allow a full complement of chromosomal loci to be analyzed on the same population of cells. Advantages of this protocol include gaining more cytogenetic information from small samples and eliminating the problem of intratumor variability.
authors.affiliation: Department of Laboratory Medicine, University of California, San Francisco 94143-0808, USA.
authors.forename: L
authors.initials: L
authors.lastname: Epstein
authors.forename: S
authors.initials: S
authors.lastname: DeVries
authors.forename: F M
authors.initials: FM
authors.lastname: Waldman
mesh_terms: D002503:Centromere / Q000235:genetics; D002875:Chromosomes / Q000235:genetics; D015342:DNA Probes; D004276:DNA, Satellite / Q000032:analysis; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D007963:Leukocytes, Mononuclear / Q000166:cytology; D008856:Microscopy, Fluorescence; D009693:Nucleic Acid Hybridization; D013048:Specimen Handling
chemical_list: D015342:DNA Probes; D004276:DNA, Satellite