Functional characterisation of the Drosophila 5-HTdro1 and 5-HTdro2B serotonin receptors in insect cells: activation of a G(alpha) s-like protein by 5-HTdro1 but lack of coupling to inhibitory G-proteins by 5-HTdro2B.
Insect cells are routinely used for the production of receptor proteins. Expression of the Drosophila 5-HTdro1 serotonin receptor resulted in positive coupling of the receptor to adenylyl cyclase via the G(alpha)s G-protein subtype. The Drosophila 5-HTdro2B receptor stimulated the metabolism of inositol phospholipid via a pertussis toxin-insensitive G-protein, but exhibited no detectable inhibition of adenylyl cyclase. Immunoblot analysis of the endogenous G-proteins revealed that Sf9 cells lack the G-protein subtypes G(alpha i 1-3) and G(alpha)o, but express the subtype G(alpha)s and G(alpha)q.
Obosi
L A
LA
School of Biological and Molecular Sciences, Oxford Brookes University, UK.
Schuette
D G
DG
Europe-Finner
G N
GN
Beadle
D J
DJ
Hen
R
R
King
L A
LA
Bermudez
I
I
D000818:Animals; D016367:Baculoviridae; D001921:Brain / Q000378:metabolism; D002460:Cell Line; D000242:Cyclic AMP / Q000378:metabolism; D004330:Drosophila / Q000378:metabolism*; D029721:Drosophila Proteins; D005260:Female; D019204:GTP-Binding Proteins / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism*; D006801:Humans; D015151:Immunoblotting; D007294:Inositol / Q000378:metabolism; D007700:Kinetics; D008562:Membrane Glycoproteins / Q000096:biosynthesis / Q000378:metabolism*; D009215:Myometrium / Q000378:metabolism; D011247:Pregnancy; D051381:Rats; D011985:Receptors, Serotonin / Q000096:biosynthesis / Q000378:metabolism*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000378:metabolism; D012701:Serotonin / Q000378:metabolism; D018411:Spodoptera; D014162:Transfection
C098813:5-HT7 protein, Drosophila; C098814:5HT-dro2B receptor; D029721:Drosophila Proteins; D008562:Membrane Glycoproteins; D011985:Receptors, Serotonin; D011994:Recombinant Proteins; D012701:Serotonin; D007294:Inositol; D000242:Cyclic AMP; D019204:GTP-Binding Proteins