title: Regulation of choline acetyltransferase/lacZ fusion gene expression in putative cholinergic neurons of Drosophila melanogaster.
abstract: We have analyzed the distribution of putative cholinergic neurons in whole-mount preparations of adult Drosophila melanogaster. Putative cholinergic neurons were visualized by X-gal staining of P-element transformed flies carrying a fusion gene consisting of 5' flanking DNA from the choline acetyltransferase (ChAT) gene and lacZ reporter gene. We have previously demonstrated that cryostat sections of transgenic flies carrying 7.4 kb of ChAT 5' flanking DNA show reporter gene expression in a pattern essentially similar to the known distribution of ChAT protein. Whole-mount staining of these same flies by X-gal should thus represent the overall distribution of ChAT-positive neurons. Extensive staining was observed in the cephalic, thoracic, and stomodeal ganglia, primary sensory neurons in antenna, maxillary palps, labial palps, leg, wing, and male genitalia. Primary sensory neurons associated with photoreceptors and tactile receptors were not stained. We also examined the effects of partial deletions of the 7.4 kb fragment on reporter gene expression. Deletion of the 7.4 kb fragment to 1.2 kb resulted in a dramatic reduction of X-gal staining in the peripheral nervous system (PNS). This indicates that important regulatory elements for ChAT expression in the PNS exist in the distal region of the 7.4 kb fragment. The distal parts of the 7.4 kb fragment, when fused to a basal heterologous promoter, can independently confer gene expression in subsets of putative cholinergic neurons. With these constructs, however, strong ectopic expression was also observed in several non-neuronal tissues.
authors.affiliation: Division of Neurosciences, Beckman Research Institute of the City of Hope, Duarte, California 91010, USA.
authors.forename: T
authors.initials: T
authors.lastname: Kitamoto
authors.forename: K
authors.initials: K
authors.lastname: Ikeda
authors.forename: P M
authors.initials: PM
authors.lastname: Salvaterra
mesh_terms: D000109:Acetylcholine / Q000502:physiology; D000818:Animals; D002795:Choline O-Acetyltransferase / Q000235:genetics*; D003001:Cloning, Molecular; D004331:Drosophila melanogaster / Q000235:genetics*; D005697:Galactosides; D017353:Gene Deletion; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D017344:Genes, Insect; D017930:Genes, Reporter; D007211:Indoles; D007763:Lac Operon; D008297:Male; D009474:Neurons / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D013194:Staining and Labeling; D014170:Transformation, Genetic; D001616:beta-Galactosidase / Q000235:genetics
chemical_list: D005697:Galactosides; D007211:Indoles; D002795:Choline O-Acetyltransferase; D001616:beta-Galactosidase; D000109:Acetylcholine; C044888:5-bromo-4-chloro-3-indolyl beta-galactoside