title: Feasibility study of a drug immunoassay based on micellar electrokinetic capillary chromatography with laser induced fluorescence detection: determination of theophylline in serum.
abstract: This paper presents principle and first results of a novel competitive binding immunoassay for monitoring of theophylline in human serum. The assay is based upon short time incubation of a mixture of antiserum, containing the antibody raised against theophylline, fluorescein labelled theophylline (tracer) and serum prior to injection of a few nanoliter of this mixture onto a fused-silica capillary for subsequent separation and analysis of free tracer and the antibody-tracer-complex by micellar electrokinetic capillary chromatography with laser induced fluorescence detection. Quantitation based upon multi-level calibration using the height of the peak produced by the free tracer is shown to provide theophylline serum levels which are in agreement with those obtained by a commercial fluorescence polarization immunoassay and with those determined by micellar elektrokinetic capillary chromatography with direct serum injection and on-column UV absorption detection.
authors.affiliation: Department of Clinical Pharmacology, University of Bern, Switzerland.
authors.forename: L
authors.initials: L
authors.lastname: Steinmann
authors.forename: J
authors.initials: J
authors.lastname: Caslavska
authors.forename: W
authors.initials: W
authors.lastname: Thormann
mesh_terms: D001667:Binding, Competitive; D002197:Capillary Action; D002845:Chromatography / Q000379:methods*; D016903:Drug Monitoring; D004563:Electrochemistry; D005240:Feasibility Studies; D016231:Fluorescence Polarization Immunoassay / Q000379:methods*; D006801:Humans; D007700:Kinetics; D007834:Lasers; D008823:Micelles; D012016:Reference Values; D013806:Theophylline / Q000097:blood*
chemical_list: D008823:Micelles; D013806:Theophylline