title: Reconstitution of Rab9 endosomal targeting and nucleotide exchange using purified Rab9-GDP dissociation inhibitor complexes and endosome-enriched membranes.
authors.affiliation: Department of Biochemistry, Stanford University School of Medicine, California 94305, USA.
authors.forename: T
authors.initials: T
authors.lastname: Soldati
authors.forename: A D
authors.initials: AD
authors.lastname: Shapiro
authors.forename: S R
authors.initials: SR
authors.lastname: Pfeffer
mesh_terms: D000818:Animals; D002458:Cell Fractionation / Q000379:methods; D002460:Cell Line; D002499:Centrifugation, Density Gradient / Q000379:methods; D003001:Cloning, Molecular / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D011992:Endosomes / Q000378:metabolism* / Q000648:ultrastructure; D020558:GTP Phosphohydrolases / Q000302:isolation & purification* / Q000378:metabolism*; D019204:GTP-Binding Proteins / Q000302:isolation & purification* / Q000378:metabolism*; D020730:Guanine Nucleotide Dissociation Inhibitors; D016244:Guanosine 5'-O-(3-Thiotriphosphate) / Q000378:metabolism; D015151:Immunoblotting / Q000379:methods; D007202:Indicators and Reagents; D008099:Liver / Q000378:metabolism* / Q000648:ultrastructure; D017368:Protein Prenylation; D011865:Radioisotope Dilution Technique; D051381:Rats; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000378:metabolism; D018411:Spodoptera; D013462:Sulfur Radioisotopes; D014162:Transfection / Q000379:methods; D020691:rab GTP-Binding Proteins
chemical_list: C086796:GDP dissociation inhibitor 1; D020730:Guanine Nucleotide Dissociation Inhibitors; D007202:Indicators and Reagents; D011994:Recombinant Proteins; D013462:Sulfur Radioisotopes; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D020558:GTP Phosphohydrolases; D019204:GTP-Binding Proteins; C489802:Rab9a protein, rat; D020691:rab GTP-Binding Proteins