Immunoaffinity column clean-up for the high-performance liquid chromatographic determination of aflatoxins B1, B2, G1, G2, M1 and Q1 in urine.
A method for the determination of aflatoxins B1, B2, G1, G2, M1 and Q1 in human urine has been developed. The 10-ml urine samples were automatically cleaned up on immunoaffinity columns and analysed by high-performance liquid chromatography (HPLC), including post-column derivatization with bromine and fluorescence detection. Average aflatoxin recoveries were: B1 103%, B2 106%, G1 98% and G2 96% in the range 6.8-73 pg/ml of urine and M1 103% and Q1 100% in the range 18-97 pg/ml of urine. The relative standard deviations were all between 1% and 21%. The determination limits of aflatoxins in urine were 6.8 pg/ml for B1, B2, G1 and G2 and 18 pg/ml for M1 and Q1.
Kussak
A
A
National Institute of Occupational Health, Analytical Chemistry Division, Umeå, Sweden.
Andersson
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B
Andersson
K
K
D016604:Aflatoxin B1 / Q000652:urine; D016607:Aflatoxin M1 / Q000652:urine; D000348:Aflatoxins / Q000652:urine*; D002273:Carcinogens / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000706:statistics & numerical data; D006801:Humans; D007158:Immunologic Techniques; D015203:Reproducibility of Results
D000348:Aflatoxins; D002273:Carcinogens; C027955:aflatoxin G1; C029754:aflatoxin G2; C033759:aflatoxin Q1; D016607:Aflatoxin M1; C029753:aflatoxin B2; D016604:Aflatoxin B1