title: Study of a viral-dual infection in rainbow trout (Oncorhynchus mykiss) by seroneutralization, western blot and polymerase chain reaction assays.
abstract: Viral-dual infections in fish are of interest to aquaculture practices but they are rarely described and studied. Several methods were applied in this work to demonstrate a case of coinfection in a reared rainbow trout (Oncorhynchus mykiss) population. Inoculation in cell cultures and cross-neutralization tests were the standard procedures that made it possible to isolate and identify a birnavirus, the infectious pancreatic necrosis virus (IPNV), and suspect of a second virus. Western blotting with both polyclonal and monoclonal antibodies, and reverse transcriptional-polymerase chain reaction (RT-PCR) demonstrate coexistence of both, IPNV and a rhabdovirus.
authors.affiliation: Departamento de Microbiología Molecular, Centro de Investigaciones Biológicas, CSIC, Madrid, España.
authors.forename: S
authors.initials: S
authors.lastname: Rodríguez
authors.forename: M P
authors.initials: MP
authors.lastname: Vilas
authors.forename: M
authors.initials: M
authors.lastname: Alonso
authors.forename: S I
authors.initials: SI
authors.lastname: Pérez
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D017756:Aquaculture; D001483:Base Sequence; D018175:Birnaviridae Infections / Q000150:complications / Q000401:mortality / Q000662:veterinary* / Q000821:virology; D015153:Blotting, Western; D002460:Cell Line; D003588:Cytopathogenic Effect, Viral; D004196:Disease Outbreaks / Q000662:veterinary*; D005260:Female; D005393:Fish Diseases / Q000401:mortality / Q000821:virology*; D018061:Infectious pancreatic necrosis virus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D017686:Oncorhynchus mykiss / Q000821:virology*; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000032:analysis; D011817:Rabbits; D012209:Rhabdoviridae / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D018353:Rhabdoviridae Infections / Q000150:complications / Q000401:mortality / Q000662:veterinary* / Q000821:virology; D013696:Temperature; D014776:Virus Cultivation
chemical_list: D000956:Antigens, Viral; D012367:RNA, Viral