title: Plakoglobin: kinetics of synthesis, phosphorylation, stability, and interactions with desmoglein and E-cadherin.
abstract: We have analyzed the kinetics of synthesis, phosphorylation, and stability of the soluble and insoluble plakoglobin (PG) and their interactions with Dsg1 and E-cadherin in Madin-Darby canine kidney (MDCK) epithelial cells in the absence of cell adhesion and after the induction of cell-cell contact. Using a combination of biochemical and morphological approaches, we show that newly synthesized PG enters a soluble:insoluble pool of proteins in a 60:40 ratio regardless of cell-cell contact. Following synthesis, PG is increasingly found in the insoluble pool. Although cell-cell contact does not effect either the size of each pool or the rate or efficiency of the transfer from the soluble into the insoluble pool, it results in a significant increase in the metabolic stability of the newly synthesized insoluble PG. The soluble PG initially forms separate complexes with E-cadherin and Dsg1. PG-Dsg1 complexes become insoluble and localize to the desmosome. PG-E-cadherin complexes remain soluble and are distributed intracellularly. The insoluble PG and E-cadherin detected at the cell periphery remain distinctly separate, as demonstrated previously [Hinck et al., 1994: J. Cell Biol. 125:1327-1340; Nathke et al., 1994: J. Cell Biol. 125:1341-1352]. In addition, we detected a separate pool of PG which is not associated with either Dsg1 or E-cadherin and after the induction of cell-cell contact becomes primarily insoluble and is distributed along the lateral membrane. Phosphorylation analysis showed that there is a significantly greater amount of phosphorylated PG in the soluble pool than in the insoluble pool. In addition the soluble pool is both serine and threonine phosphorylated, whereas the insoluble PG is primarily phosphorylated on serine residues.
authors.affiliation: Department of Anatomy and Cell Biology, University of Alberta, Edmonton, Canada.
authors.forename: M
authors.initials: M
authors.lastname: Pasdar
authors.forename: Z
authors.initials: Z
authors.lastname: Li
authors.forename: V
authors.initials: V
authors.lastname: Chlumecky
mesh_terms: D000818:Animals; D000918:Antibody Specificity; D015820:Cadherins / Q000378:metabolism*; D002448:Cell Adhesion / Q000502:physiology; D002460:Cell Line / Q000166:cytology / Q000378:metabolism; D003598:Cytoskeletal Proteins / Q000096:biosynthesis / Q000276:immunology / Q000378:metabolism*; D051182:Desmogleins; D051180:Desmoplakins; D003896:Desmosomes / Q000276:immunology / Q000378:metabolism*; D004285:Dogs; D019084:Fluorescent Antibody Technique, Indirect; D015151:Immunoblotting; D007686:Kidney Tubules, Distal / Q000166:cytology; D007700:Kinetics; D010766:Phosphorylation; D015398:Signal Transduction / Q000502:physiology; D012995:Solubility; D051185:gamma Catenin
chemical_list: D015820:Cadherins; D003598:Cytoskeletal Proteins; D051182:Desmogleins; D051180:Desmoplakins; D051185:gamma Catenin