Structural characterization of U*-1915 in domain IV from Escherichia coli 23S ribosomal RNA as 3-methylpseudouridine.
Mass spectrometry-based methods have been used to study post-transcriptional modification in the 1900-1974 nt segment of domain IV in 23S rRNA of Escherichia coli, a region which interacts with domain V in forming the three- dimensional structure of the peptidyl transferase center within the ribosome. A nucleoside constituent of M r 258 (U*)which occurs at position 1915, within the highly modified oligonucleotide sequence 1911-psiAACU*Apsi-1917, was characterized as 3-methylpseudouridine (m3psi). The assignment was confirmed by chemical synthesis of m3psi and comparison with the natural nucleoside by liquid chromatography-mass spectrometry. 3-Methylpseudouridine is previously unknown in nature and is the only known derivative of the common modified nucleoside pseudouridine thus far found in bacterial rRNA.
Kowalak
J A
JA
Department of Biochemistry, University of Utah, Salt Lake City, UT 84132, USA.
Bruenger
E
E
Hashizume
T
T
Peltier
J M
JM
Ofengand
J
J
McCloskey
J A
JA
D001483:Base Sequence; D004926:Escherichia coli / Q000737:chemistry*; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D015394:Molecular Structure; D011560:Pseudouridine / Q000031:analogs & derivatives* / Q000737:chemistry; D012338:RNA, Ribosomal, 23S / Q000737:chemistry*
D012338:RNA, Ribosomal, 23S; D011560:Pseudouridine; C034686:3-methylpseudouridine