title: Salmonella typhimurium acrB-like gene: identification and role in resistance to biliary salts and detergents and in murine infection.
abstract: Salmonella serotype typhimurium transpositional mutants altered in resistance to biliary salts and detergents were isolated previously. We have characterized further the LX1054 mutant strain, the most sensitive of them. The chromosomal DNA segment flanking transposon insertion was cloned and sequenced. The highest level of identity was found for the acrB (formerly acrE) gene of Escherichia coli, a gene encoding a drug efflux pump of the Acr family. LX1054 exhibited a reduced capacity to colonize the intestinal tract. After passages in mice, the mutant strain lost the sensitive phenotype. In vitro, a resumption of growth appeared after 17 h of culture in medium with cholate or other tested biological or chemical detergents. Then, the acquired resistant phenotype seemed stable. The data suggested a role of S. typhimurium acrB-like gene in resistance to biliary salts and detergents and in mice intestinal colonization. However, the local and transient sensitivity observed in vivo, and the in vitro adaptations suggest that several detergent-resistance mechanisms operate in S. typhimurium.
authors.affiliation: INRA, Centre de Tours-Nouzilly, Pathologie Infectieuse et Immunologie, France.
authors.forename: F J
authors.initials: FJ
authors.lastname: Lacroix
authors.forename: A
authors.initials: A
authors.lastname: Cloeckaert
authors.forename: O
authors.initials: O
authors.lastname: Grépinet
authors.forename: C
authors.initials: C
authors.lastname: Pinault
authors.forename: M Y
authors.initials: MY
authors.lastname: Popoff
authors.forename: H
authors.initials: H
authors.lastname: Waxin
authors.forename: P
authors.initials: P
authors.lastname: Pardon
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics* / Q000502:physiology; D001483:Base Sequence; D002352:Carrier Proteins; D002793:Cholic Acids / Q000494:pharmacology*; D003001:Cloning, Molecular; D004352:Drug Resistance, Microbial; D029968:Escherichia coli Proteins; D005260:Female; D005798:Genes, Bacterial; D007422:Intestines / Q000382:microbiology; D008565:Membrane Proteins / Q000235:genetics* / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D027425:ATP-Binding Cassette, Sub-Family C Proteins; D009154:Mutation; D012481:Salmonella Infections, Animal / Q000382:microbiology*; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics / Q000472:pathogenicity / Q000502:physiology*; D017422:Sequence Analysis, DNA; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; D002793:Cholic Acids; D029968:Escherichia coli Proteins; D008565:Membrane Proteins; D027425:ATP-Binding Cassette, Sub-Family C Proteins; D012967:Sodium Dodecyl Sulfate; C083496:AcrB protein, E coli; C028213:3-((3-cholamidopropyl)dimethylammonium)-1-propanesulfonate