title: Evidence for UV-B-induced DNA degradation in Euglena gracilis mediated by activation of metal-dependent nucleases.
abstract: It is demonstrated that in vivo irradiation with artificial UV-B for several hours significantly reduces the amount of large DNA extractable from immobilized Euglena in comparison with non-irradiated controls. This UV-B effect can be eliminated by a drastic reduction of the divalent ion concentration in the extracellular medium, i.e. the substitution of the culture medium by Tris-buffered agarose. Moreover, in vitro degradation of large DNA is demonstrated for crude protein extracts isolated from non-irradiated or UV-B-irradiated Euglena. The nuclease activity is shown for both crude protein extracts and purified nucleases; in both cases, two protein bands possessing nuclease activity are obtained with apparent molecular masses of 26 and 40 kDa and their activity is inhibited by specific nuclease inhibitors, i.e. aurintricarboxylic acid and ATP, applied at a concentration as low as 10(-8) M. Moreover, in vitro, nuclease activity clearly depends on the pH, with an optimum around pH 4.5, and on the ion composition of the extracellular medium. A strong stimulating effect is shown for Ca2+ with an optimum around 10(-4) M; this effect is potentiated by Zn2+ and Mn2+, but strongly counteracted by Mg2+ and the calmodulin inhibitors trifluoperazine and N- (6-aminohexyl)-5-chloro-1-naphthalenesulphonamide (W5). These results favour the concept which explains the lethal UV-B effect on Euglena as arising from a change in the general metabolic state of the cell and an activation of a DNA-degrading system, i.e. activation of metal-dependent nucleases (U.K. Tirlapur, D.-P. Häder and R. Scheuerlein, UV-B mediated damage in the photosynthetic flagellate, Euglena gracilis, studied by image analysis, Beitr. Biol. Pflanzen, 67 (1992) 305-317).
authors.affiliation: Institut für Botanik und Pharmazeutische Biologie, Friedrich-Alexander-Universität Erlangen-Nürnberg, Germany.
authors.forename: R
authors.initials: R
authors.lastname: Scheuerlein
authors.forename: S
authors.initials: S
authors.lastname: Treml
authors.forename: B
authors.initials: B
authors.lastname: Thar
authors.forename: U K
authors.initials: UK
authors.lastname: Tirlapur
authors.forename: D P
authors.initials: DP
authors.lastname: Häder
mesh_terms: D000818:Animals; D004249:DNA Damage; D016054:DNA, Protozoan / Q000302:isolation & purification / Q000378:metabolism / Q000528:radiation effects*; D003851:Deoxyribonucleases / Q000378:metabolism* / Q000528:radiation effects; D004789:Enzyme Activation / Q000528:radiation effects; D005056:Euglena gracilis / Q000201:enzymology / Q000235:genetics / Q000528:radiation effects*; D008670:Metals / Q000378:metabolism; D014466:Ultraviolet Rays
chemical_list: D016054:DNA, Protozoan; D008670:Metals; D003851:Deoxyribonucleases