title: Effect of low dose UVB irradiation on the migratory properties and functional capacities of human skin dendritic cells.
abstract: We recently described the 'spontaneous' migration of skin dendritic cells out of human split skin during culture. Since newly infiltrating cells from the circulation are excluded, this in vitro model is very suitable for studying the effect of UVB irradiation on the migratory properties, phenotype and functional capacities of skin cells. In the present study, we show that UVB irradiation of the skin before the culture period results in a significantly lower number of migrated cells that could be obtained compared with untreated skin. Relatively more dendritic cells of the population that migrated from UVB-irradiated skin were of dermal origin, as indicated by a higher percentage of CD1b+ cells. These data imply that UVB irradiation inhibits migration, especially of the epidermal Langerhans cells. Ultrastructural analysis of the irradiated skin revealed that the UVB dose used did not cause any directly visible damage to the cells. However, the cell population that had migrated from UVB-irradiated skin showed a significantly lower capacity to stimulate allogeneic T cells. This was not due to a lower expression of MHC class II on these cells. The percentage of cells expressing B7.1, B7.2 and LFA-3 was decreased in the population migrated from irradiated skin. The possible mechanism underlying the UVB-induced suppression is discussed.
authors.affiliation: Department of Cell Biology and Immunology, Faculty of Medicine, Vrije Universiteit, Amsterdam, The Netherlands.
authors.forename: C D
authors.initials: CD
authors.lastname: Richters
authors.forename: E A
authors.initials: EA
authors.lastname: Reits
authors.forename: A M
authors.initials: AM
authors.lastname: Van Pelt
authors.forename: M J
authors.initials: MJ
authors.lastname: Hoekstra
authors.forename: J
authors.initials: J
authors.lastname: Van Baare
authors.forename: J S
authors.initials: JS
authors.lastname: Du Pont
authors.forename: E W
authors.initials: EW
authors.lastname: Kamperdijk
mesh_terms: D000938:Antigen-Presenting Cells / Q000528:radiation effects; D002465:Cell Movement; D046508:Culture Techniques; D003713:Dendritic Cells / Q000276:immunology / Q000528:radiation effects*; D005260:Female; D006801:Humans; D016130:Immunophenotyping; D007801:Langerhans Cells / Q000276:immunology / Q000528:radiation effects; D008213:Lymphocyte Activation; D012867:Skin / Q000166:cytology / Q000276:immunology / Q000528:radiation effects*; D013601:T-Lymphocytes / Q000276:immunology; D014466:Ultraviolet Rays