Quantification of fecal carbohydrates by near-infrared reflectance analysis.
Established methods for quantitative analysis of fecal carbohydrates (CHO) are time consuming, require unpopular sample handling, and are therefore rarely performed. The aim of this study was to evaluate the efficiency, validity, and practicability of near-infrared reflectance analysis (NIRA), compared with standard methods for measurement of fecal CHO. Excretion of fecal CHO was cross- validated spectrophotometrically with the anthrone method. Fecal CHO concentrations ranged form 2.7 to 24.5 g/kg wet weight. Methods comparison showed linear regression over the entire range of diagnostic relevance with a correlation coefficient of 0.869 (S(y/x) +/- 0.31). Repeated measurements from the same stool collections obviated the need for homogenization. These results indicate that NIRA may be a new, reliable, and accurate test in the diagnosis of CHO malabsorption.
Stein
J
J
Division of Gastroenterology, Department of Internal Medicine, Johann-Wolfgang-Goethe-University, Frankfurt, Germany.
Purschian
B
B
Zeuzem
S
S
Lembcke
B
B
Caspary
W F
WF
D000293:Adolescent; D000328:Adult; D000368:Aged; D000873:Anthracenes; D002241:Carbohydrates / Q000032:analysis*; D002648:Child; D002675:Child, Preschool; D005243:Feces / Q000737:chemistry*; D005260:Female; D006801:Humans; D016014:Linear Models; D008286:Malabsorption Syndromes / Q000175:diagnosis; D008297:Male; D008875:Middle Aged; D013055:Spectrophotometry, Infrared / Q000379:methods*; D000095322:Anthrones
D000873:Anthracenes; D002241:Carbohydrates; C004522:anthrone; D000095322:Anthrones