title: Unscheduled DNA synthesis following excimer laser ablation of the cornea in vivo.
abstract: The amount of unscheduled DNA synthesis (UDS) produced in the cells adjacent to excimer laser ablations in the cornea was compared for 193-nm and 248-nm laser wavelengths. UDS is interpreted to indicate the process of excision repair of pyrimidine dimers formed in DNA. 193-nm laser ablation did not produce a statistically significant difference in the amount of UDS as compared to a negative control (diamond-knife corneal incision). However, 248-nm laser ablation did produce a highly statistically significant difference in the amount of UDS as compared to both the negative control (P < 0.001) and the 193-nm laser irradiation (P < 0.001). Other forms of DNA damage (single-strand DNA chain breaks and DNA-protein crosslinks) are not measurable by UDS and need to be investigated in the evaluation of the oncogenic potential of 193-nm laser ablation.
authors.affiliation: Laser Research Laboratory, Massachusetts Eye and Ear Infirmary, Boston, Massachusetts 02114, USA.
authors.forename: R C
authors.initials: RC
authors.lastname: Nuss
authors.forename: C A
authors.initials: CA
authors.lastname: Puliafito
authors.forename: E
authors.initials: E
authors.lastname: Dehm
mesh_terms: D000818:Animals; D002452:Cell Count; D003315:Cornea / Q000473:pathology / Q000528:radiation effects* / Q000601:surgery; D004247:DNA / Q000096:biosynthesis*; D004249:DNA Damage / Q000528:radiation effects; D004261:DNA Replication / Q000528:radiation effects*; D004848:Epithelium / Q000473:pathology / Q000528:radiation effects; D054018:Lasers, Excimer; D018903:Photorefractive Keratectomy / Q000009:adverse effects*; D011817:Rabbits; D014466:Ultraviolet Rays
chemical_list: D004247:DNA