Flavonoid inhibition of enzymic and nonenzymic lipid peroxidation in rat liver differs from its influence on the glutathione-related enzymes.
Eight flavonoids were tested for their antiperoxidative activities against lipid peroxidation induced in liver cell membranes either by nonenzymic way (ascorbic acid-Fe2+ system, FeAs) or by enzymic way (arachidonic acid, AA). When lipid peroxidation is induced by FeAs, the order in the inhibitory potency for the different flavonoids assayed is: (-)-epicatechin approximately luteolin > quercetin approximately (+)-catechin > delphinidin > kaempferol >> apigenin > naringenin. However, when lipid peroxidation is induced by AA, the potency order is markedly modified: delphinidin > (-)-epicatechin > (+)-catechin > kaempferol > quercetin > luteolin > naringenin > apigenin. These flavonoids were also tested for their influence on glutathione-related enzymes, which constitute one of the aim physiological antioxidant systems. It is concluded that the antiperoxidative effect shown by most of the flavonoids is exerted without modifying these enzymes.
Galvez
J
J
Department of Pharmacology, School of Pharmacy, University of Granada, Spain.
de la Cruz
J P
JP
Zarzuelo
A
A
Sanchez de la Cuesta
F
F
D000818:Animals; D003864:Depression, Chemical; D005419:Flavonoids / Q000737:chemistry / Q000494:pharmacology*; D005978:Glutathione / Q000378:metabolism*; D005979:Glutathione Peroxidase / Q000037:antagonists & inhibitors; D005980:Glutathione Reductase / Q000037:antagonists & inhibitors; D005982:Glutathione Transferase / Q000037:antagonists & inhibitors; D066298:In Vitro Techniques; D015227:Lipid Peroxidation / Q000187:drug effects*; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D051381:Rats; D017208:Rats, Wistar
D005419:Flavonoids; D005979:Glutathione Peroxidase; D005980:Glutathione Reductase; D005982:Glutathione Transferase; D005978:Glutathione