GERI-BP002-A, novel inhibitor of acyl-CoA: cholesterol acyltransferase produced by Aspergillus fumigatus F93.
A new inhibitor of acyl-CoA:cholesterol acyltransferase (ACAT), designated GERI-BP002-A, was isolated from the culture broth of Aspergillus fumigatus F93 by acetone extraction, EtOAc extraction, SiO2 column chromatography and reverse phase HPLC. Spectroscopic analyses of the compound identified bis (2-hydroxy-3-tert-butyl-5-methylphenyl) methane as the structure and its molecular weight and formula to be 340 and C23H32O2, respectively. GERI-BP002-A inhibited ACAT activity by 50% at the concentration of 50 microM in an enzyme assay system using rat liver microsomes.
Kim
Y K
YK
Bioproducts Research Group, Korea Research Institute of Bioscience & Biotechnology, KIST, Yusung , Korea.
Lee
H W
HW
Son
K H
KH
Kwon
B M
BM
Jeong
T S
TS
Lee
D H
DH
Shin
J
J
Seo
Y
Y
Kim
S U
SU
Bok
S H
SH
D001232:Aspergillus fumigatus / Q000145:classification / Q000378:metabolism*; D002084:Butylated Hydroxytoluene / Q000031:analogs & derivatives* / Q000302:isolation & purification; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D005285:Fermentation; D006801:Humans; D002785:Sterol O-Acyltransferase / Q000037:antagonists & inhibitors*; D014407:Tumor Cells, Cultured
D004791:Enzyme Inhibitors; D002084:Butylated Hydroxytoluene; D002785:Sterol O-Acyltransferase; C008437:2,2'-methylenebis(4-methyl-6-tert-butylphenol)