Blastomere derivation and domains of gene expression in the Spemann Organizer of Xenopus laevis.
Spemann's Organizer, located in the dorsal marginal zone of the amphibian gastrula, induces and differentiates dorsal axial structures characteristic of this and other vertebrates. To trace the cellular origins of the Xenopus Organizer, we labelled dorsal blastomeres of three of the four tiers (A, B and C) of the 32-cell embryo with green, red and blue fluorescent lineage tracers. A strong vegetalward displacement of labelled clones occurs between the late blastula and early gastrula stages but clones mix only slightly at their borders. The typical early gastrula Organizer is composed of approximately 10% A1 progeny in its animalmost region, 70% B1 progeny in the central region, and 20% C1 progeny in vegetal and deep regions. Variability in the composition of the early gastrula Organizer results from variability in the position of early cleavage planes and in pregastrulation movements. As the Organizer involutes during gastrulation, forming dorsal axial mesoderm, clonal boundaries are greatly dispersed by cell intermixing. Within a clone, deep cells are displaced and intermixed more than superficial cells. Variability in the distribution of progeny in the dorsal axial mesoderm of the late gastrula results mostly from variable intermixing of cells during gastrulation. Experiments to perturb later developmental events by molecular or embryonic manipulations at an early stage must take this variability into account along with the majority distributions of the fate map. Within the early gastrula Organizer, the genes Xbra, goosecoid, noggin and xNR3 are expressed differently in the animal-vegetal and superficial-deep dimensions. In situ hybridization and lineage labelling define distinct regions of the dorsal marginal zone. By the end of gastrulation, dorsal axial mesoderm cells derived from the Organizer have altered their expression of the genes Xbra, goosecoid, noggin and xNR3. At a given stage, a cell's position in the embryo rather than its lineage may be more important in determining which genes it will express.
Vodicka
M A
MA
University of California, Berkeley 94720, USA.
Gerhart
J C
JC
D000818:Animals; D001757:Blastomeres / Q000166:cytology / Q000502:physiology*; D002352:Carrier Proteins; D002454:Cell Differentiation; D019070:Cell Lineage; D004268:DNA-Binding Proteins / Q000235:genetics; D005326:Fetal Proteins / Q000235:genetics; D005775:Gastrula / Q000166:cytology / Q000502:physiology*; D015870:Gene Expression; D005796:Genes / Q000502:physiology; D051956:Goosecoid Protein; D018398:Homeodomain Proteins; D017403:In Situ Hybridization; D008648:Mesoderm / Q000166:cytology / Q000502:physiology; D011506:Proteins / Q000235:genetics; D018125:Receptors, Transforming Growth Factor beta / Q000235:genetics; D012097:Repressor Proteins; D020825:T-Box Domain Proteins; D014157:Transcription Factors; D016212:Transforming Growth Factor beta; D029867:Xenopus Proteins; D014982:Xenopus laevis / Q000196:embryology* / Q000235:genetics; D000098908:Brachyury Protein; D000099176:Noggin Protein
D002352:Carrier Proteins; D004268:DNA-Binding Proteins; D005326:Fetal Proteins; D051956:Goosecoid Protein; D018398:Homeodomain Proteins; D011506:Proteins; D018125:Receptors, Transforming Growth Factor beta; D012097:Repressor Proteins; D020825:T-Box Domain Proteins; D014157:Transcription Factors; D016212:Transforming Growth Factor beta; D029867:Xenopus Proteins; D000098908:Brachyury Protein; D000099176:Noggin Protein; C094620:nodal3.1 protein, Xenopus