Immunocytochemical localization of acetylated alpha-tubulin in mouse resident peritoneal macrophages.
Antibodies which recognize tubulin, actin and vimentin were used to localize microtubules, microfilaments and intermediate filaments, respectively, in whole Triton X-100 extracted macrophages. The cytoskeleton components were seen by transmission electron microscopy of cells labeled with secondary antibodies coupled to colloidal gold particles. All microtubules were labeled with a monoclonal antibody which recognizes alpha tubulin. In addition, few were labeled with an antibody recognizing acetylated alpha tubulin.
Del Caro
L M
LM
Departamento de Histologia e Embriologia, Universidade Federal do Rio de Janeiro, Brazil.
de Souza
W
W
D000107:Acetylation; D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism; D000918:Antibody Specificity; D002478:Cells, Cultured; D003902:Detergents / Q000737:chemistry; D019084:Fluorescent Antibody Technique, Indirect; D017979:Gold Colloid / Q000737:chemistry / Q000378:metabolism; D007150:Immunohistochemistry; D017737:Macrophages, Peritoneal / Q000737:chemistry* / Q000166:cytology / Q000648:ultrastructure; D051379:Mice; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D017830:Octoxynol / Q000737:chemistry; D014404:Tubulin / Q000032:analysis*
D000911:Antibodies, Monoclonal; D003902:Detergents; D017979:Gold Colloid; D014404:Tubulin; D017830:Octoxynol