Prolactin induces switching of T-cell receptor gene expression from alpha to gamma in rat Nb2 pre-T lymphoma cells(1)
Prolactin (PRL) is considered to be an immunomodulator. This study was conducted to analyze the actions of PRL on the gene expressions for T-cell receptor (TCR) chains in a fat pre-T lymphoma cell line, Nb2. PRL remarkably stimulated the gene expression for TCR gamma chain within 4 h and suppressed TCR alpha chain mRNA expression within 8 h in Nb2 cells. The expression of TCR beta mRNA in the cells was concomitantly, but to a less extent, repressed by the hormone treatment. These results suggest that PRL may participate in the regulation of mammalian T-cell differentiation and development.
Hosokawa
Y
Y
Department of Biochemistry, Mie University Faculty of Medicine, Tsu, Japan.
Yang
M
M
Kaneko
S
S
Tanaka
M
M
Nakashima
K
K
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D017931:DNA Primers; D018076:DNA, Complementary; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D015723:Gene Library; D016399:Lymphoma, T-Cell; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011388:Prolactin / Q000494:pharmacology*; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D051381:Rats; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000096:biosynthesis*; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000096:biosynthesis*; D015183:Restriction Mapping; D014407:Tumor Cells, Cultured
D017931:DNA Primers; D018076:DNA, Complementary; D012333:RNA, Messenger; D016693:Receptors, Antigen, T-Cell, alpha-beta; D016692:Receptors, Antigen, T-Cell, gamma-delta; D011388:Prolactin