Binding of amino acids to the cationic surfactant cetyltrimethylammoniumbromide.
The interaction of amino acids with the cationic surfactant cetyltrimethylammoniumbromide (CTAB) was studied by charge transfer reversed-phase thin-layer chromatography and the relative strength of interaction was calculated. In the majority of cases the surfactant has a negligible effect on the hydrophobicity of amino acids. Only the binding of Arg, Glu, Gly, Leu, Lys, Met, Nle, Phe Trp, Tyr and Val to the surfactant was observed, however, the strength of interaction was fairly low. The hydrophobicity of the amino acid side chains significantly influenced the strength of interaction. The findings support the hypothesis that the binding of cationic surfactants to proteins involves more than one amino acid residues and that the hydrophobic forces have a considerable impact on the interaction.
Cserháti
T
T
Central Research Institute for Chemistry, Hungarian Academy of Sciences, Budapest, Hungary.
Forgács
E
E
D000596:Amino Acids / Q000737:chemistry*; D002412:Cations; D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000737:chemistry*; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002855:Chromatography, Thin Layer / Q000379:methods*; D000432:Methanol / Q000737:chemistry; D012044:Regression Analysis; D012995:Solubility; D013501:Surface-Active Agents / Q000737:chemistry*; D014867:Water / Q000737:chemistry
D000596:Amino Acids; D002412:Cations; D002593:Cetrimonium Compounds; D013501:Surface-Active Agents; D014867:Water; D000432:Methanol; D000077286:Cetrimonium