title: Aggressive papillary tumors of the temporal bone: an immunohistochemical analysis in tissue culture.
abstract: Aggressive papillary tumors of the temporal bone are neoplasms that are locally invasive and destructive. Previously classified on histologic study as middle ear adenomas or adenocarcinomas, observational evidence suggested that they arose from endolymphatic sac. To evaluate this hypothesis, we established a tissue culture from cells derived from such a papillary tumor and compared immunohistochemical stains of the original tumor with stains on endolymphatic epithelium. Similarities in expression of neuroectodermal antigens were observed. Similar staining antigens in cells derived from tumor and the endolymphatic sac provide evidence that epithelium from endolymphatic sac is the site of origin for these aggressive neoplasms. In tissue culture the cells remain contact inhibited and dependent on serum or growth factors with survival beyond the expected senescence at 30 to 50 generations. Therefore the cell culture technique provides a model for study of the disruption of growth control and invasive properties of this tumor.
authors.affiliation: Department of Otolaryngology/Head & Neck Surgery, Albert Einstein College of Medicine, Bronx, N.Y., USA.
authors.forename: R J
authors.initials: RJ
authors.lastname: Levin
authors.forename: J G
authors.initials: JG
authors.lastname: Feghali
authors.forename: N
authors.initials: N
authors.lastname: Morganstern
authors.forename: J
authors.initials: J
authors.lastname: Llena
authors.forename: M K
authors.initials: MK
authors.lastname: Bradley
mesh_terms: D000231:Adenocarcinoma, Papillary / Q000473:pathology*; D000328:Adult; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D012888:Skull Neoplasms / Q000473:pathology*; D013701:Temporal Bone; D014407:Tumor Cells, Cultured