Photodynamic inactivation of cell-free HIV strains by a red-absorbing chlorin-type photosensitizer.
We have investigated the photodynamic activity of a new chlorin-type photosensitizer on a reference human immunodeficiency virus type 1 (HIV-1) strain, two wild-type HIV-1 isolates and two drug-resistant HIV-1 isolates. This chlorin was highly effective for the inactivation of free viruses, as assessed by two different quantitative cell culture assays. In the absence of blood components, all the HIV strains, including wild-type and drug-resistant mutant isolates, were totally inactivated using 30 micrograms ml-1 of chlorin and 0.75 J cm-2 of 661 nm light. Successful killing of HIV-1 strains in either plasma or whole blood was also obtained by increasing the chlorin concentration moderately. Our results demonstrate the antiviral efficiency of this chlorin, suggesting the potential application of dye-sensitized photoirradiation to decontaminate blood products.
Grandadam
M
M
Laboratoire de Virologie du CERVI, CNRS EP 57, Hôpital de la Pitié-Salpêtrière, Paris, France.
Ingrand
D
D
Huraux
J M
JM
Aveline
B
B
Delgado
O
O
Vever-Bizet
C
C
Brault
D
D
D001769:Blood; D002021:Buffers; D002460:Cell Line; D002474:Cell-Free System; D002478:Cells, Cultured; D003905:Deuteroporphyrins / Q000737:chemistry / Q000494:pharmacology; D015658:HIV Infections / Q000821:virology; D015497:HIV-1 / Q000187:drug effects* / Q000302:isolation & purification / Q000528:radiation effects*; D006367:HeLa Cells; D006801:Humans; D008027:Light; D017319:Photosensitizing Agents / Q000494:pharmacology*; D010949:Plasma; D011166:Porphyrins / Q000737:chemistry / Q000494:pharmacology*
D002021:Buffers; D003905:Deuteroporphyrins; D017319:Photosensitizing Agents; D011166:Porphyrins; C006969:chlorin; C049889:hydroxyethylvinyldeuteroporphyrin