Particle enhanced immunoaggregation of F(ab')2 molecules.
The immunological agglutination reactions of physically absorbed F(ab')2 molecules onto anionic and cationic latex particles have been investigated by means of optical absorbance measurements. These measurements have been conducted under different conditions to determine the most influential factors. Surface F(ab')2 and BSA densities, particle concentration in the reaction medium and polyethylene glycol concentration are some of these factors. Sensitized cationic and anionic latexes differ considerably with respect to their colloidal stability and reactivity. As a general rule, the sensitized cationic latex has a relatively higher colloidal stability and hence, it provides reagents with a better optical response. Less than 0.025 microgram/ml of C-reactive protein has been detected using this particle enhanced optical immunoassay.
Ortega-Vinuesa
J L
JL
Biocolloid and Fluid Physics Group, Department of Applied Physics, Faculty of Sciences, University of Granada, Spain.
Molina-Bolívar
J A
JA
Hidalgo-Alvarez
R
R
D000327:Adsorption; D000818:Animals; D002097:C-Reactive Protein / Q000276:immunology; D003102:Colloids; D007118:Immunoassay; D007140:Immunoglobulin Fab Fragments / Q000737:chemistry*; D007840:Latex / Q000737:chemistry*; D011817:Rabbits; D012542:Scattering, Radiation; D013010:Sonication
D003102:Colloids; D007140:Immunoglobulin Fab Fragments; D007840:Latex; D002097:C-Reactive Protein