title: Purification and characterization of human immunodeficiency virus type 1 reverse transcriptase.
authors.affiliation: Division of Infectious Diseases, Case Western Reserve University School of Medicine, Cleveland, Ohio 44106-4984, USA.
authors.forename: S F
authors.initials: SF
authors.lastname: Le Grice
authors.forename: C E
authors.initials: CE
authors.lastname: Cameron
authors.forename: S J
authors.initials: SJ
authors.lastname: Benkovic
mesh_terms: D002458:Cell Fractionation / Q000379:methods; D002846:Chromatography, Affinity / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000379:methods; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D004926:Escherichia coli; D054303:HIV Reverse Transcriptase; D015497:HIV-1 / Q000201:enzymology*; D006801:Humans; D007202:Indicators and Reagents; D007700:Kinetics; D008970:Molecular Weight; D012194:RNA-Directed DNA Polymerase / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D013379:Substrate Specificity; D014461:Ultracentrifugation / Q000379:methods
chemical_list: D007202:Indicators and Reagents; D011994:Recombinant Proteins; D054303:HIV Reverse Transcriptase; D012194:RNA-Directed DNA Polymerase