Overexpression of MP41 gene in a transformed endothelial cell line correlates with the increased fibronectin expression and a decreased incidence of tumorigenicity.
mp41 gene, which was originally identified as a gene regulated by the circadian clock, showed some similarity with yeast cell cycle regulator, CDC25, and its mRNA expression is restricted mainly to non-dividing cell. To elucidate the growth regulatory of mp41, mp41 cDNA was transfected into a transformed human endothelial cell line. All stable mp41 transformants showed increased levels of fibronectin expression and reduced migratory activity in vitro. Although the in vitro growth rate of mp41 transformants and their ability to grow in soft agar were not significantly altered, their tumor formation was suppressed significantly in nude mice. The results imply that mp41 gene overexpression altered the tumorigenicity associated with fibronectin elevation in stable transformation of the transformed endothelial cell line.
Kondo
M
M
Cell Biology Laboratory, National Institute of Bioscience and Human-Technology, MITI, Tsukuba Science City, Japan.
Watanabe
M
M
Amanuma
K
K
Oka
S
S
Ishida
N
N
D000818:Animals; D002455:Cell Division; D002460:Cell Line; D002461:Cell Line, Transformed; D002465:Cell Movement; D002471:Cell Transformation, Neoplastic; D002940:Circadian Rhythm; D004730:Endothelium, Vascular / Q000378:metabolism; D005353:Fibronectins / Q000096:biosynthesis*; D015870:Gene Expression; D006801:Humans; D007700:Kinetics; D051379:Mice; D008819:Mice, Nude; D009374:Neoplasms, Experimental / Q000473:pathology*; D014176:Protein Biosynthesis; D011506:Proteins / Q000502:physiology*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000378:metabolism; D013997:Time Factors; D014162:Transfection; D014183:Transplantation, Heterologous
D005353:Fibronectins; C092005:Ppnr protein, mouse; D011506:Proteins; D012333:RNA, Messenger; D011994:Recombinant Proteins