Fibronectin induces protease dependent focal matrix depletion and cell overlap in cultured rheumatoid synoviocytes.
OBJECTIVE
To characterize the effect of added fibronectin (Fn) on human rheumatoid synoviocytes (RAS).


METHODS
Early passage cultured RAS were studied by fluorescent imaging microscopy with multiple parameter overlaying and confocal laser scanning microscopy.


RESULTS
Added Fn induced a localized decrease in matrix Fn at sites of cell overlap. Similar matrix depletion could be induced by collagen VI, cell binding (120 k) and heparin binding (60 k) fragments of Fn, but not by gelatin binding fragment (45 k).


CONCLUSIONS
The decrease in matrix Fn was associated with the induction of a transformation-like phenotype of overlapping cell growth. Both phenomena were inhibited by serine protease inhibitors.
Wang
A Z
AZ
Department of Medicine, Western Pennsylvania Hospital, Pittsburgh 15224, USA.
Zhang
X R
XR
Wang
J C
JC
Fisher
G W
GW
Diamond
H S
HS
D001172:Arthritis, Rheumatoid / Q000378:metabolism / Q000473:pathology*; D002455:Cell Division / Q000502:physiology; D002478:Cells, Cultured / Q000166:cytology; D002918:Chymotrypsin / Q000037:antagonists & inhibitors; D003094:Collagen / Q000187:drug effects / Q000378:metabolism; D003470:Culture Media; D005109:Extracellular Matrix / Q000737:chemistry / Q000187:drug effects* / Q000473:pathology; D005347:Fibroblasts / Q000473:pathology; D005353:Fibronectins / Q000494:pharmacology*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D018613:Microscopy, Confocal; D008875:Middle Aged; D010003:Osteoarthritis / Q000473:pathology; D010446:Peptide Fragments / Q000494:pharmacology; D015842:Serine Proteinase Inhibitors / Q000494:pharmacology*; D013583:Synovial Membrane / Q000166:cytology* / Q000378:metabolism; D013997:Time Factors; D014361:Trypsin Inhibitors / Q000494:pharmacology
D003470:Culture Media; D005353:Fibronectins; D010446:Peptide Fragments; D015842:Serine Proteinase Inhibitors; D014361:Trypsin Inhibitors; D003094:Collagen; D002918:Chymotrypsin