title: Increase in dopamine turnover and tyrosine hydroxylase enzyme in hippocampus of rats fed on low selenium diet.
abstract: We have studied the turnover of dopamine, noradrenaline, and serotonin and their metabolites in hippocampus of adult female rats that were fed control or selenium-deficient diets during 15 days. Under these circumstances, there was an increase of dopamine turnover (4-fold) in rats fed with selenium-deficient diet with respect to controls and also an increase in the tyrosine hydroxylase activity (75.8%), which was the result of the increase of the amount of the enzyme (2-fold), without significant change in the phosphorylation of the tyrosine hydroxylase. In addition the glutathione peroxidase, glutathione reductase, catalase, and superoxide dismutase activities have been studied. After selenium-deficient diet, the enzymatic activities of superoxide dismutase and catalase did not show change with respect to the controls; however glutathione reductase and glutathione peroxidase significantly decreased 15% and 29%, respectively. It is concluded that the increase in dopamine turnover seems to be associated with the induction of tyrosine hydroxylase enzyme. In these conditions the decrease in antioxidant capacity may produce a cascade of events, which accelerates the degenerative process, since the increase in dopamine turnover produces an increase in oxygen radical by monoamine oxidase activity.
authors.affiliation: Departamento de Bioquimica, Bromatologia y Toxicologia, Facultad de Farmacia, Universidad de Sevilla, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Castaño
authors.forename: A
authors.initials: A
authors.lastname: Ayala
authors.forename: J A
authors.initials: JA
authors.lastname: Rodriguez-Gomez
authors.forename: C P
authors.initials: CP
authors.lastname: de la Cruz
authors.forename: E
authors.initials: E
authors.lastname: Revilla
authors.forename: J
authors.initials: J
authors.lastname: Cano
authors.forename: A
authors.initials: A
authors.lastname: Machado
mesh_terms: D015102:3,4-Dihydroxyphenylacetic Acid / Q000378:metabolism; D000818:Animals; D001679:Biogenic Amines / Q000096:biosynthesis; D004032:Diet; D004298:Dopamine / Q000378:metabolism*; D004435:Eating / Q000502:physiology; D005260:Female; D006624:Hippocampus / Q000201:enzymology / Q000378:metabolism*; D006897:Hydroxyindoleacetic Acid / Q000378:metabolism; D015151:Immunoblotting; D008996:Monoamine Oxidase Inhibitors / Q000494:pharmacology; D010293:Pargyline / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D012643:Selenium / Q000008:administration & dosage / Q000172:deficiency*; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism*; D015430:Weight Gain / Q000502:physiology
chemical_list: D001679:Biogenic Amines; D008996:Monoamine Oxidase Inhibitors; D015102:3,4-Dihydroxyphenylacetic Acid; D006897:Hydroxyindoleacetic Acid; D010293:Pargyline; D014446:Tyrosine 3-Monooxygenase; D012643:Selenium; D004298:Dopamine