title: Binding site for basic drugs on alpha 1-acid glycoprotein as revealed by chemometric analysis of biochromatographic data.
abstract: Interactions between alpha 1-acid glycoprotein (AGP) and 52 basic drugs were quantified by means of high-performance liquid chromatography (HPLC). The HPLC retention parameters were related quantitatively to the hydrophobicity and molecular modelling parameters, giving rise to the prediction of relative drug-AGP binding from the chemical structure of a drug. A structural model of one binding site on AGP, common for various classes of drugs, was defined which accounted for the observed and reported differences in binding to AGP. A combination of biochromatography and chemometrics has been presented as a promising new approach in biochemical/pharmacological studies.
authors.affiliation: Department of Biopharmaceutics and Pharmacodynamics, Medical University, Gdańsk, Poland.
authors.forename: R
authors.initials: R
authors.lastname: Kaliszan
authors.forename: A
authors.initials: A
authors.lastname: Nasal
authors.forename: M
authors.initials: M
authors.lastname: Turowski
mesh_terms: D001665:Binding Sites; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D009961:Orosomucoid / Q000737:chemistry* / Q000378:metabolism; D011955:Receptors, Drug / Q000737:chemistry* / Q000378:metabolism; D012822:Silicon Dioxide; D013056:Spectrophotometry, Ultraviolet; D013329:Structure-Activity Relationship
chemical_list: D009961:Orosomucoid; D011955:Receptors, Drug; D012822:Silicon Dioxide