title: Molecular cloning and RARE cleavage mapping of human 2p, 6q, 8q, 12q, and 18q telomeres.
abstract: Large terminal fragments of human chromosomes 2p, 6p, 8q, 12q, and 18q were cloned using yeast artificial chromosomes (YACs). RecA-assisted restriction endonuclease (RARE) cleavage analysis of genomic DNA samples from II unrelated individuals using YAC-derived probes confirmed the telomeric localizations of the half-YACs studied. The cloned fragments provide telomeric closure of maps for the respective chromosome arms and will supply the reagents needed for analyzing and sequencing these distal subtelomeric regions.
authors.affiliation: Wistar Institute, Philadelphia, Pennsylvania 19104, USA.
authors.forename: R A
authors.initials: RA
authors.lastname: Macina
authors.forename: K
authors.initials: K
authors.lastname: Morii
authors.forename: X L
authors.initials: XL
authors.lastname: Hu
authors.forename: D G
authors.initials: DG
authors.lastname: Negorev
authors.forename: C
authors.initials: C
authors.lastname: Spais
authors.forename: L A
authors.initials: LA
authors.lastname: Ruthig
authors.forename: H C
authors.initials: HC
authors.lastname: Riethman
mesh_terms: D001483:Base Sequence; D018244:Chromosomes, Artificial, Yeast; D002877:Chromosomes, Human / Q000235:genetics* / Q000648:ultrastructure; D002881:Chromosomes, Human, Pair 12 / Q000235:genetics / Q000648:ultrastructure; D002887:Chromosomes, Human, Pair 18 / Q000235:genetics / Q000648:ultrastructure; D002889:Chromosomes, Human, Pair 2 / Q000235:genetics / Q000648:ultrastructure; D002896:Chromosomes, Human, Pair 6 / Q000235:genetics / Q000648:ultrastructure; D002898:Chromosomes, Human, Pair 8 / Q000235:genetics / Q000648:ultrastructure; D003001:Cloning, Molecular; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping; D016615:Telomere / Q000235:genetics*