title: Role of a peptide tagging system in degradation of proteins synthesized from damaged messenger RNA.
abstract: Variants of lambda repressor and cytochrome b562 translated from messenger RNAs without stop codons were modified by carboxyl terminal addition of an ssrA-encoded peptide tag and subsequently degraded by carboxyl terminal-specific proteases present in both the cytoplasm and periplasm of Escherichia coli. The tag appears to be added to the carboxyl terminus of the nascent polypeptide chain by cotranslational switching of the ribosome from the damaged messenger RNA to ssrA RNA.
authors.affiliation: Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139-4307, USA.
authors.forename: K C
authors.initials: KC
authors.lastname: Keiler
authors.forename: P R
authors.initials: PR
authors.lastname: Waller
authors.forename: R T
authors.initials: RT
authors.lastname: Sauer
mesh_terms: D000409:Alanine; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018388:Codon, Terminator; D003573:Cytochrome b Group / Q000235:genetics / Q000378:metabolism*; D004268:DNA-Binding Proteins; D010450:Endopeptidases / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D029968:Escherichia coli Proteins; D008969:Molecular Sequence Data; D010455:Peptides / Q000378:metabolism; D014176:Protein Biosynthesis; D011499:Protein Processing, Post-Translational; D012329:RNA, Bacterial / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D014764:Viral Proteins; D054334:Viral Regulatory and Accessory Proteins
chemical_list: D018388:Codon, Terminator; D003573:Cytochrome b Group; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; D010455:Peptides; D012329:RNA, Bacterial; D012333:RNA, Messenger; D012097:Repressor Proteins; D014764:Viral Proteins; D054334:Viral Regulatory and Accessory Proteins; C016649:phage repressor proteins; C096222:tmRNA; C017850:cytochrome b562, E coli; D010450:Endopeptidases; C072135:C-terminal processing peptidase; D000409:Alanine