The synthetic retinoid N-(4-hydroxyphenyl) retinamide (4-HPR) exerts antiproliferative and apoptosis-inducing effects in the androgen-independent human prostatic JCA-1 cells.
A significant reduction in JCA-1 cell proliferation was observed as a result of treatment with 4-HPR. This was accompanied by the down-regulated expression of the proliferating cell nuclear antigen PCNA, cyclins D and E, and p34cdc2. Unexpectedly, p53 and pRB was also suppressed in response to 4-HPR. A prolonged exposure to 4-HPR (6 days) promoted the appearance of non-adherent cells showing morphological features and DNA fragmentation patterns indicative of apoptosis.
Hsieh
T C
TC
Department of Biochemistry, New York Medical College, Valhalla 10595, USA.
Ng
C
C
Wu
J M
JM
D000728:Androgens / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects; D016213:Cyclins / Q000032:analysis; D015536:Down-Regulation; D017313:Fenretinide / Q000494:pharmacology*; D006801:Humans; D008297:Male; D008934:Mitogens / Q000037:antagonists & inhibitors; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D011471:Prostatic Neoplasms / Q000188:drug therapy* / Q000473:pathology; D014407:Tumor Cells, Cultured
D000728:Androgens; D000970:Antineoplastic Agents; D016213:Cyclins; D008934:Mitogens; D018809:Proliferating Cell Nuclear Antigen; D017313:Fenretinide