Induction of proteins in response to cold acclimation of rainbow trout cells.
The in vitro translation of poly(A)-RNA isolated from control and cold-treated cells showed that low temperatures induced changes in the population of translatable mRNAs. When cellular proteins extracted from cold-treated cells were subjected to two-dimensional gel electrophoresis, the 70 kDa protein was found to be synthesized during the cold treatment. N-terminal sequence analysis showed that the 70 kDa cold-inducible protein was a homolog of the mammalian valosin-containing protein and yeast CDC48p. The changes in mRNA and protein contents during cold acclimation may result from the expression of genes involved in the adjustment of cellular metabolism to low temperature or the induced proteins may be directly involved in the cold acclimation.
Yamashita
M
M
National Research Institute of Fisheries Science, Yokohama, Japan.
Ojima
N
N
Sakamoto
T
T
D000064:Acclimatization / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D003080:Cold Temperature; D005786:Gene Expression Regulation / Q000502:physiology; D008969:Molecular Sequence Data; D008970:Molecular Weight; D017686:Oncorhynchus mykiss / Q000235:genetics / Q000502:physiology*; D014176:Protein Biosynthesis; D011506:Proteins / Q000737:chemistry / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D017421:Sequence Analysis; D017386:Sequence Homology, Amino Acid
D011506:Proteins; D012333:RNA, Messenger