title: Cloning and characterization of the rice CatA catalase gene, a homologue of the maize Cat3 gene.
abstract: We isolated and sequenced a genomic clone (CatA) encoding CAT-A catalase, a homologue of the maize catalase isozyme 3 (CAT-3) from rice (Oryza sativa L.). The 5'-upstream non-coding region had very low similarity with the maize Cat3 gene and possible cis elements and sequence motifs in the maize Cat3 gene were not evident, except for TATA and CAAT motifs. Several sequence motifs found in the promoters of plant seed-specific genes were identified in the 5'-upstream non-coding region of the CatA gene. Northern blotting showed that the CatA gene is expressed at high levels in seeds during early development and also in young seedlings. Methyl viologen (paraquat) resulted in the 3-fold induction of the CatA gene in the leaves of young seedlings, whereas abscisic acid, wounding, salicylic acid, and hydrogen peroxide had no or only slight effects. The 1.9 kb 5'-upstream fragment (-1559 to +342) of the CatA gene was fused with the Escherichia coli beta-glucuronidase (GUS) gene and introduced by electroporation into protoplasts prepared from rice suspension-cultured cells, then the transient expression of the GUS gene was examined. Deletion analysis of this chimeric gene suggested that a weak silencer is located in the region between -1564 to -699. Abscisic acid (ABA) at a final concentration of 10(-6) M doubled GUS activity in protoplasts electroporated with the chimeric DNAs having 1.9 to 1.2 kb 5'-upstream regions. A sequence highly similar to the Sph box, a motif found in genes modulated by ABA, was found at -266 to -254. Deletion of this region however, did not eliminate the responsiveness to ABA. Expression of the chimeric gene in the protoplasts was not enhanced by stress such as low and high temperature, hydrogen peroxide, methyl viologen, salicylic acid, elicitor, and UV light. The chimeric CatA-GUS plasmid DNAs amplified in the methylation-positive strain, E. coli DH5alpha, showed GUS gene activities, whereas all the chimeric DNAs amplified in the methylation-deficient E. coli JM110 were completely inactive in the presence or absence of ABA in the culture medium. DNA methylation, especially of either one or both of the deoxyadenosines at the two GATC motifs (one in the first exon and the other in the first intron of the rice CatA gene), appeared to be responsible for the CatA promoter activity identified in the transient assay.
authors.affiliation: Department of Molecular Biology, National Institute of Agrobiological Resources, Tsukuba, Ibaraki 305, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Higo
authors.forename: H
authors.initials: H
authors.lastname: Higo
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology; D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D002374:Catalase / Q000235:genetics*; D003001:Cloning, Molecular; D018744:DNA, Plant; D003624:Darkness; D015870:Gene Expression / Q000187:drug effects; D017343:Genes, Plant; D005966:Glucuronidase; D007438:Introns; D008745:Methylation; D008969:Molecular Sequence Data; D012275:Oryza / Q000201:enzymology* / Q000235:genetics; D011401:Promoter Regions, Genetic; D011523:Protoplasts; D017384:Sequence Deletion; D012689:Sequence Homology, Nucleic Acid; D013696:Temperature; D003313:Zea mays / Q000201:enzymology / Q000235:genetics
chemical_list: D018744:DNA, Plant; D000040:Abscisic Acid; D002374:Catalase; D005966:Glucuronidase