Similarity among the Drosophila (6-4)photolyase, a human photolyase homolog, and the DNA photolyase-blue-light photoreceptor family.
Ultraviolet light (UV)-induced DNA damage can be repaired by DNA photolyase in a light-dependent manner. Two types of photolyase are known, one specific for cyclobutane pyrimidine dimers (CPD photolyase) and another specific for pyrimidine (6-4) pyrimidone photoproducts[(6-4)photolyase]. In contrast to the CPD photolyase, which has been detected in a wide variety of organisms, the (6-4)photolyase has been found only in Drosophila melanogaster. In the present study a gene encoding the Drosophila(6-4)photolyase ws cloned, and the deduced amino acid sequence of the product was found to be similar to the CPD photolyase and to the blue-light photoreceptor of plants. A homolog of the Drosophila (6-4)photolyase gene was also cloned from human cells.
Todo
T
T
Radiation Biology Center, Kyoto University, Kyoto, Japan.
Ryo
H
H
Yamamoto
K
K
Toh
H
H
Inui
T
T
Ayaki
H
H
Nomura
T
T
Ikenaga
M
M
D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D004260:DNA Repair; D018076:DNA, Complementary / Q000235:genetics; D004255:Deoxyribodipyrimidine Photo-Lyase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D004331:Drosophila melanogaster / Q000201:enzymology* / Q000235:genetics; D005182:Flavin-Adenine Dinucleotide / Q000378:metabolism; D017344:Genes, Insect; D006801:Humans; D008027:Light; D008969:Molecular Sequence Data; D017956:Photoreceptor Cells, Invertebrate / Q000737:chemistry*; D010940:Plant Proteins / Q000737:chemistry*; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D016415:Sequence Alignment; D014466:Ultraviolet Rays
D018076:DNA, Complementary; D010940:Plant Proteins; D011994:Recombinant Proteins; D005182:Flavin-Adenine Dinucleotide; C098727:pyrimidine(6-4)pyrimidone photolyase; D004255:Deoxyribodipyrimidine Photo-Lyase