title: Haplotype test reveals departure from neutrality in a segment of the white gene of Drosophila melanogaster.
abstract: Restriction map studies previously revealed extensive linkage disequilibria in the transcriptional unit of the white locus in natural Drosophila melanogaster populations. To understand the causes of these disequilibria, we sequenced a 4722-bp region of the white gene from 15 lines of D. melanogaster and 1 line of Drosophila simulans. Statistical tests applied to the entire 4722-bp region do not reject neutrality. In contrast, a test for high-frequency haplotypes ("Haplotype test") revealed an 834-bp segment, encompassing the 3' end of intron 1 to the 3' end of intron 2, in which the structure of variation deviates significantly from the predictions of a neutral equilibrium model. The variants in this 834-bp segment segregate as single haplotype blocks. We proposed that these unusually large haplotype blocks are due to positive selection on polymorphisms within the white gene, including a replacement polymorphism, Arg-->Leu, within this segment.
authors.affiliation: Department of Zoology, University of Maryland, College Park 20742, USA. dkirby@zool.umd.edu
authors.forename: D A
authors.initials: DA
authors.lastname: Kirby
authors.forename: W
authors.initials: W
authors.lastname: Stephan
mesh_terms: D018528:ATP-Binding Cassette Transporters; D000818:Animals; D001483:Base Sequence; D004247:DNA; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000235:genetics*; D005136:Eye Proteins; D005260:Female; D006239:Haplotypes; D007301:Insect Hormones / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D011110:Polymorphism, Genetic; D011995:Recombination, Genetic; D013045:Species Specificity
chemical_list: D018528:ATP-Binding Cassette Transporters; D029721:Drosophila Proteins; D005136:Eye Proteins; D007301:Insect Hormones; C059254:w protein, Drosophila; D004247:DNA