title: Cholecystokinin induced signaling in rat glioma C6 cells.
abstract: We investigated the effect of sulphated cholecystokinin octapeptide (CCK-8S) on free intracellular calcium concentration, phosphatidylinositol metabolism, and protein phosphorylation in C6 cells, a rat glioma cell line which was shown to express CCKB type receptors. Increase in [Ca2+]i by both influx across the cell membrane and release from internal stores was demonstrated by utilizing a laser confocal imaging system. Because CCK-8S produced a transient elevation of inositol triphosphate level participation of InsP3 in calcium signaling in C6 cells is very likely. Protein kinase C seems to be involved in CCK-8S induced signaling in rat glioma C6 cells as demonstrated by using in vivo phosphorylation experiments.
authors.affiliation: Max Planck Gesellschaft, Research Unit Pharmacological Haemostaseology, Friedrich Schiller University, Germany.
authors.forename: R
authors.initials: R
authors.lastname: Kaufmann
authors.forename: T
authors.initials: T
authors.lastname: Schöneberg
authors.forename: C
authors.initials: C
authors.lastname: Lindschau
authors.forename: H
authors.initials: H
authors.lastname: Haller
authors.forename: T
authors.initials: T
authors.lastname: Ott
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005910:Glioma / Q000378:metabolism*; D007295:Inositol Phosphates / Q000378:metabolism; D010716:Phosphatidylinositols / Q000378:metabolism; D010750:Phosphoproteins / Q000378:metabolism; D010766:Phosphorylation; D011493:Protein Kinase C / Q000378:metabolism; D051381:Rats; D015398:Signal Transduction / Q000187:drug effects*; D012844:Sincalide / Q000031:analogs & derivatives* / Q000494:pharmacology; D014407:Tumor Cells, Cultured
chemical_list: C090019:8-sulfocholecystokinin octapeptide; D007295:Inositol Phosphates; D010716:Phosphatidylinositols; D010750:Phosphoproteins; D011493:Protein Kinase C; D012844:Sincalide; D002118:Calcium