Effects of gamma-alumina and hydroxyapatite coatings on the growth and metabolism of human osteoblasts.
The behavior of human osteoblasts cultivated on hydroxyapatite or alumina-coated disks of Ti6AL4V was studied in vitro. Cell anchorage and spreading were observed by scanning electron microscopy. Cell growth was monitored by counting cells and measuring DNA at 5 h and 2,5, and 10 days after cell seeding. Cells grown for 10 days were labeled with 14C-proline and total protein and collagen synthesis were measured; the type of collagen was also determined. Both ceramics showed excellent biocompatibility. At 10 days of culture the cells showed a higher rate of proliferation on alumina than on hydroxyapatite. Neither ceramic altered the collagen biosynthesis or the osteoblast-like properties of the cells, as indicated by the high percentage of type I collagen.
Labat
B
B
Laboratoire de Biochimie, Faculté de médecine, Saint Etienne, France.
Chamson
A
A
Frey
J
J
D000537:Aluminum Oxide / Q000494:pharmacology*; D001672:Biocompatible Materials; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis; D004247:DNA / Q000096:biosynthesis; D006801:Humans; D006882:Hydroxyapatites / Q000494:pharmacology*; D006909:Hydroxyproline / Q000378:metabolism; D007700:Kinetics; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000187:drug effects* / Q000378:metabolism; D011392:Proline / Q000378:metabolism; D014176:Protein Biosynthesis
D001672:Biocompatible Materials; D006882:Hydroxyapatites; D003094:Collagen; D004247:DNA; D011392:Proline; D000537:Aluminum Oxide; D006909:Hydroxyproline