Stress causes induction of MAP kinase-specific phosphatase and rapid repression of MAP kinase activity in Drosophila.
Heat shock and chemical stress induce activation of heat shock (stress) genes and synthesis of heat shock proteins. It is not yet fully understood which molecular mechanism leads to activation. Probably denatured proteins play an important role in activating the transcription factor (HSF), but on the other hand there are many hints that a phosphorylation event is involved, too. During a search for a possible signal transduction system in Drosophila Schneider 2 cells we analysed the response of the mitogen-activated protein (MAP) kinase after stress and its regulation by phosphatases. We show here that stress activates a MAP kinase-specific phosphatase in Drosophila and inhibits MAP kinase activity.
Cornelius
G
G
Institute for Genetics, Saarland University, Federal Republic of Germany.
Engel
M
M
D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000494:pharmacology; D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000502:physiology; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000378:metabolism*; D002460:Cell Line; D017868:Cyclic AMP-Dependent Protein Kinases / Q000502:physiology; D004330:Drosophila / Q000201:enzymology* / Q000502:physiology; D004790:Enzyme Induction / Q000187:drug effects / Q000502:physiology; D018869:Heat-Shock Response / Q000502:physiology*; D007476:Ionophores / Q000494:pharmacology; D010749:Phosphoprotein Phosphatases / Q000096:biosynthesis / Q000378:metabolism*; D010766:Phosphorylation; D011493:Protein Kinase C / Q000502:physiology; D012333:RNA, Messenger / Q000096:biosynthesis; D015398:Signal Transduction / Q000502:physiology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D007476:Ionophores; D012333:RNA, Messenger; D015124:8-Bromo Cyclic Adenosine Monophosphate; D000001:Calcimycin; D017868:Cyclic AMP-Dependent Protein Kinases; D011493:Protein Kinase C; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D010749:Phosphoprotein Phosphatases; D013755:Tetradecanoylphorbol Acetate; D002118:Calcium