Cadmium accumulation and metallothionein expression in brain of mice at different stages of development.
Cadmium accumulation and metallothionein (MT) expression were studied in brains of adult mice as well as at different stages of development. MT expression was also studied in the eye of adult mice as well as at different stages of development. Using northern blot analysis with total RNA, MT mRNAs were not detected in day 16 through day 18 embryos or in postnatal animals up to day 14. Detectable expression of MT-I, -II, and -III mRNAs was obtained in brains of 30- and 60-day-old-mice. The expression of MT-III mRNA appeared to be much stronger in adults (12 weeks old or more) than in 30- and 60-day-old animals. In contrast, there was similar expression of MT-I and -II in 30- and 60-day-old mice. Cd distribution to brain was found to decrease with age; the brains of 7-day-old mice contained about 4-times more Cd than that of adult mice. Thus, an inverse correlation was observed between MT expression and Cd accumulation in brain.
Choudhuri
S
S
Department of Pharmacology, Toxicology and Therapeutics, University of Kansas Medical Center, Kansas City, KS 66160-7417, USA.
Liu
W L
WL
Berman
N E
NE
Klaassen
C D
CD
D000818:Animals; D000831:Animals, Newborn; D001483:Base Sequence; D015152:Blotting, Northern; D001921:Brain / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*; D002104:Cadmium / Q000493:pharmacokinetics*; D004622:Embryo, Mammalian / Q000378:metabolism; D005123:Eye / Q000196:embryology / Q000254:growth & development / Q000378:metabolism; D005260:Female; D005333:Fetus / Q000196:embryology; D008297:Male; D008668:Metallothionein / Q000096:biosynthesis* / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D011247:Pregnancy; D012333:RNA, Messenger / Q000096:biosynthesis; D014018:Tissue Distribution
D012333:RNA, Messenger; D002104:Cadmium; D008668:Metallothionein