Antiproliferative effect of a prostatic cell-derived activity on the human androgen-dependent prostatic carcinoma cell line LNCaP.
We have identified a new antiproliferative activity from the conditioned medium of two androgen-independent prostatic cancer cell lines, PC3 and DU-145. This antiproliferative activity selectively inhibited cell proliferation of an androgen-dependent prostate cancer cell line LNCaP in a dose-dependent manner. No antiproliferative activity was observed against mouse fibroblast 3T3, normal human lymphocytes, human leukemic cells, including promyelocyte HL-60 or T cell HUT-78, or human adenocarcinoma cell lines, including prostatic cells JCA-1, ovary NIH:OVCAR-3, cervix C-33A, or breast MDA-MB-231. Cell cycle analysis revealed that the antiproliferative activity did not induce apoptosis in LNCaP cells, but it prevented some G1 LNCaP cells from entering into the S phase of the cell cycle. The antiproliferative activity was sensitive to high temperature (100 degrees C) and to proteinase digestion; however, it was resistant to 56 degrees C, pH 2.0, and reducing agent treatment, as well as to DNase and RNase digestion. The antiproliferative activity was partially purified by gel filtration, ion-exchange chromatography, and SDS-PAGE, with an apparent molecular weight of 50 kD. The antiproliferative activity was not affected by neutralizing antibody against TGF-beta 1,2,3, TNF-alpha, PDGF, EGF, IL-1, IL-2, IL-3, IL-4, or IL-6.
Abolhassani
M
M
Department of Medicine, New York Medical College, Valhalla 10595, USA.
Chiao
J W
JW
D000728:Androgens / Q000378:metabolism*; D002277:Carcinoma / Q000276:immunology / Q000378:metabolism*; D002453:Cell Cycle; D002455:Cell Division; D017077:Culture Media, Conditioned; D016207:Cytokines / Q000378:metabolism; D003851:Deoxyribonucleases / Q000494:pharmacology; D004229:Dithiothreitol / Q000494:pharmacology; D010450:Endopeptidases / Q000494:pharmacology; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008297:Male; D009500:Neutralization Tests; D011471:Prostatic Neoplasms / Q000276:immunology / Q000378:metabolism*; D012260:Ribonucleases / Q000494:pharmacology; D013696:Temperature; D014407:Tumor Cells, Cultured
D000728:Androgens; D017077:Culture Media, Conditioned; D016207:Cytokines; D003851:Deoxyribonucleases; D012260:Ribonucleases; D010450:Endopeptidases; D004229:Dithiothreitol