Isolation of the B alpha and B beta mating-type loci of Schizophyllum commune.
The B mating type of the basidiomycete fungus, Schizophyllum commune is determined by two, tightly linked, multi-specificity (also called multi-allelic) loci: B alpha and B beta. A plasmid library was used in DNA-mediated transformation to obtain transformants that displayed B-directed development. Plasmids that conferred B alpha 1 and B beta 1 mating-type specificities were rescued from the transformants. Fragments of DNA from each plasmid hybridized to genomic DNA from the strain used to make the plasmid library; however, they did not hybridize, or hybridized only weakly, to genomic DNA from strains with mating-type specificities different from B alpha 1 or B beta 1. The cloned fragments are presumed to correspond to active regions of each B mating-type locus.
Specht
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Center for Cancer Research, Massachusetts Institute of Technology, Cambridge 02139, USA.
D015139:Blotting, Southern; D003001:Cloning, Molecular; D005800:Genes, Fungal / Q000235:genetics*; D049770:Genes, Mating Type, Fungal; D010957:Plasmids; D015183:Restriction Mapping; D012567:Schizophyllum / Q000235:genetics*; D014170:Transformation, Genetic / Q000235:genetics