title: Comparison of three methods for competitive binding of monoclonal antibodies. The localization of antigenic sites for monoclonal antibodies on Panulirus interruptus hemocyanin.
abstract: The competitive binding of a panel of monoclonal antibodies against hemocyanin of Panulirus interruptus hemocyanin was investigated with three different methods. A competitive-binding immunoassay method was more successful in the determination of ternary complexes than gel electrophoresis and gel filtration. The latter two methods could only be applied with antibodies possessing a higher affinity. Eleven monoclonal antibodies were assigned to groups on the basis of their interactions with five antigenic regions.
authors.affiliation: Biochemisch Laboratorium, Rijksuniversiteit Groningen, Netherlands.
authors.forename: F G
authors.initials: FG
authors.lastname: Perton
authors.forename: J H
authors.initials: JH
authors.lastname: Dijkema
authors.forename: T
authors.initials: T
authors.lastname: Smilda
authors.forename: B
authors.initials: B
authors.lastname: Erik van Uffelen
authors.forename: J J
authors.initials: JJ
authors.lastname: Beintema
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D001666:Binding Sites, Antibody; D001667:Binding, Competitive; D002850:Chromatography, Gel; D003445:Crustacea / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D018604:Epitope Mapping / Q000379:methods*; D006433:Hemocyanins / Q000737:chemistry / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D006433:Hemocyanins