Blockade of the discriminative stimulus effects of gamma-hydroxybutyric acid (GHB) by the GHB receptor antagonist NCS-382.
The present study was designed to assess the ability of the newly synthetized, selective gamma-hydroxybutyric acid (GHB) receptor antagonist, NCS-382, in blocking the discriminative stimulus effects of GHB in a T-maze, food-reinforced drug discrimination procedure. Two groups of rats were trained to run the left arm of the maze 30 min after the i.g. administration of either 300 or 700 mg/kg GHB and the right arm after water. Once discrimination was acquired, combination of different doses of NCS-382 (0, 12.5, 25.0 and 50.0 mg/kg, IP) and GHB training doses were tested for blockade of GHB discrimination. NCS-382 dose-dependently blocked GHB-appropriate responding in both the 300 and 700 mg/kg GHB rat groups. The results of the present study indicate that the discriminative stimulus properties of GHB are mediated via stimulation of GHB receptors.
Colombo
G
G
Dipartimento di Neuroscienze "Bernard B. Brodie", Università di Cagliari, Italy.
Agabio
R
R
Bourguignon
J
J
Fadda
F
F
Lobina
C
C
Maitre
M
M
Reali
R
R
Schmitt
M
M
Gessa
G L
GL
D000818:Animals; D000927:Anticonvulsants / Q000494:pharmacology*; D001070:Appetitive Behavior / Q000187:drug effects*; D001567:Benzocycloheptenes / Q000494:pharmacology*; D001921:Brain / Q000187:drug effects; D004193:Discrimination Learning / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D008297:Male; D018782:Maze Learning / Q000187:drug effects*; D009474:Neurons / Q000187:drug effects; D051381:Rats; D011956:Receptors, Cell Surface / Q000037:antagonists & inhibitors*; D012978:Sodium Oxybate / Q000037:antagonists & inhibitors* / Q000494:pharmacology
C039403:4-hydroxybutyric acid receptor; D000927:Anticonvulsants; D001567:Benzocycloheptenes; D011956:Receptors, Cell Surface; C066471:NCS 382; D012978:Sodium Oxybate