The role of glutathione in the transport and catabolism of nitric oxide.
Nitric oxide acts as a neuronal and vascular messenger implying diffusion through intracellular environments containing 5-10 mM glutathione. Nitric oxide reacts with glutathione under aerobic conditions generating S-nitrosoglutathione (GSNO). GSNO reacts with glutathione (k= 8.3 X 10(-3) M-1 X s-1) to generate nitrous oxide and glutathione disulfide (GSSG). Anaerobically, glutathione reacts with nitric oxide generating nitrous oxide and GSSG (k= 4.8 X 10(-4) s-1 at 5 mM GSH). In both aerobic and anaerobic situations the nitroxyl anion may be an intermediate in the synthesis of nitrous oxide and, under aerobic conditions, nitroxyl anion may generate peroxynitrite. We present a hypothesis for the intracellular interaction between nitric oxide and glutathione.
Hogg
N
N
Biophysics Research Institute, Medical College of Wisconsin, Milwaukee, 53226, USA.
Singh
R J
RJ
Kalyanaraman
B
B
D000332:Aerobiosis; D000693:Anaerobiosis; D001692:Biological Transport; D005978:Glutathione / Q000031:analogs & derivatives / Q000096:biosynthesis / Q000378:metabolism*; D019803:Glutathione Disulfide; D007700:Kinetics; D008956:Models, Chemical; D009569:Nitric Oxide / Q000096:biosynthesis / Q000378:metabolism*; D009603:Nitroso Compounds / Q000378:metabolism; D026422:S-Nitrosoglutathione
D009603:Nitroso Compounds; D009569:Nitric Oxide; D026422:S-Nitrosoglutathione; D005978:Glutathione; D019803:Glutathione Disulfide