The anti-glycolytic activity of 3-bromopyruvate on mature boar spermatozoa in vitro.
Mature epididymal boar spermatozoa converted fructose, glycerol and glycerol-3-phosphate to carbon dioxide, but in the presence of 0.5 mM 3-bromopyruvate, these oxidations were inhibited while that of lactate was unaffected. Inhibition of the oxidation of these substrates results in a decrease in the content of ATP and the accumulation of dihydroxyacetone phosphate and fructose-1,6-bisphosphate. Examination of the activities of the enzymes within stage two of the glycolytic pathway showed that glyceraldehyde-3-phosphate dehydrogenase and 3-phosphoglycerate kinase were immediately inhibited by 3-bromopyruvate in a competitive manner. We now report the action of 3-bromopyruvate on the metabolic activity of boar spermatozoa. At a concentration of 0.5 mM, this compound selectively inhibits stage two of the glycolytic pathway and becomes yet another specific inhibitor of spermatozoal metabolism.
Jones
A R
AR
Department of Biochemistry, University of Sydney, N.S.W. Australia.
Gillan
L
L
Milmlow
D
D
D000255:Adenosine Triphosphate / Q000032:analysis / Q000378:metabolism; D000818:Animals; D002245:Carbon Dioxide / Q000032:analysis / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005632:Fructose / Q000032:analysis / Q000378:metabolism; D005635:Fructosediphosphates / Q000032:analysis / Q000378:metabolism; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000037:antagonists & inhibitors; D005990:Glycerol / Q000032:analysis / Q000378:metabolism; D005994:Glycerophosphates / Q000032:analysis / Q000378:metabolism; D006019:Glycolysis / Q000187:drug effects*; D008297:Male; D010084:Oxidation-Reduction; D010735:Phosphoglycerate Kinase / Q000037:antagonists & inhibitors; D011773:Pyruvates / Q000494:pharmacology*; D013094:Spermatozoa / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D013552:Swine
D004791:Enzyme Inhibitors; D005635:Fructosediphosphates; D005994:Glycerophosphates; D011773:Pyruvates; D002245:Carbon Dioxide; D005632:Fructose; C017092:bromopyruvate; D000255:Adenosine Triphosphate; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D010735:Phosphoglycerate Kinase; D005990:Glycerol