Isolation of the toxin responsible for gousiekte, a plant-induced cardiomyopathy of ruminants in southern Africa.
Gousiekte was induced in sheep and goats by the oral and intravenous (i/v) administration of methanolic and ethanolic precipitates of aqueous extracts of Pavetta harborii. Further purification of the toxic principle was carried out by dialysis and ion-exchange chromatography. The toxin was eluted from the cation-exchange resin with 1 mol/l of ammonium acetate and finally purified on Sephadex G-10 and silica gel. Spots on thin-layer chromatographic plates (TLC) were visualized with ninhydrin. Gousiekte was induced with the pure toxin after i/v administration in goats. The yield of pure toxin was about 10 mg/kg of dried leaves.
Fourie
N
N
Division of Toxicology, Onderstepoort Veterinary Institute, South Africa.
Erasmus
G L
GL
Schultz
R A
RA
Prozesky
L
L
D000353:Africa, Southern; D000818:Animals; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D002855:Chromatography, Thin Layer; D006331:Heart Diseases / Q000209:etiology / Q000662:veterinary*; D010939:Plant Poisoning / Q000150:complications / Q000662:veterinary*; D010947:Plants, Toxic; D012418:Ruminants; D014118:Toxins, Biological / Q000302:isolation & purification*
D014118:Toxins, Biological