title: A total parenteral nutrition solution supplemented with a nucleoside and nucleotide mixture sustains intestinal integrity, but does not stimulate intestinal function after massive bowel resection in rats.
abstract: The effects of supplementing a total parenteral nutrition solution with a nucleoside and nucleotide mixture on mucosal adaptive processes after massive bowel resection were studied. Male Wistar rats (n=30) underwent 80% small intestine resection, were randomized into two groups and received either standard total parenteral nutrition (TPN) or TPN supplemented with a nucleoside and nucleotide mixture (2.5 mL.kg-1.d-1). An additional five rats, fed a nonpurified diet and not resected, were used as controls. After 4 or 7 d, rats were killed and samples were collected for mucosal indices and intestinal enzymatic activities (disaccharidases and diamine oxidase). After massive small bowel resection and TPN, residual jejunal mucosal wet weights, villus heights, protein and RNA contents on d 4 and 7, and total wet weights and DNA contents on d 7 were significantly lower than in the control group. Administration of the nucleoside and nucleotide mixture resulted in significantly higher residual jejunal total and mucosal weights, proteins, DNA, RNA contents, and the ratio of proliferating cell nuclear antigen positive cells per crypt than did the standard TPN solution on d 7. However, disaccharidase and diamine oxidase activities were not affected by supplementation with the nucleoside and nucleotide mixture. Our data suggest the supplementation of a nucleoside and nucleotide mixture to a TPN solution can attenuate the initial mucosal atrophy and improve intestinal cell turnover after massive bowel resection, but the supplementation has little effect on enterocyte enzymatic activities.
authors.affiliation: Department of Surgery II, Osaka University Medical School, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Iijima
authors.forename: T
authors.initials: T
authors.lastname: Tsujinaka
authors.forename: M
authors.initials: M
authors.lastname: Kishibuchi
authors.forename: Y
authors.initials: Y
authors.lastname: Kido
authors.forename: C
authors.initials: C
authors.lastname: Ebisui
authors.forename: K
authors.initials: K
authors.lastname: Kan
authors.forename: M
authors.initials: M
authors.lastname: Yano
authors.forename: T
authors.initials: T
authors.lastname: Mori
mesh_terms: D006631:Amine Oxidase (Copper-Containing) / Q000032:analysis; D000818:Animals; D001284:Atrophy; D001835:Body Weight; D004247:DNA / Q000032:analysis; D004186:Disaccharidases / Q000032:analysis; D002149:Energy Intake; D007413:Intestinal Mucosa / Q000737:chemistry / Q000187:drug effects / Q000473:pathology; D007421:Intestine, Small / Q000601:surgery*; D007583:Jejunum / Q000737:chemistry / Q000187:drug effects* / Q000473:pathology; D008297:Male; D009705:Nucleosides / Q000008:administration & dosage / Q000494:pharmacology*; D009711:Nucleotides / Q000008:administration & dosage / Q000494:pharmacology*; D009929:Organ Size; D010289:Parenteral Nutrition, Total / Q000009:adverse effects / Q000592:standards; D011506:Proteins / Q000032:analysis; D012313:RNA / Q000032:analysis; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
chemical_list: D009705:Nucleosides; D009711:Nucleotides; D011506:Proteins; D012313:RNA; D004247:DNA; D006631:Amine Oxidase (Copper-Containing); D004186:Disaccharidases