title: Differences in the envelope proteins of Chlamydia pneumoniae, Chlamydia trachomatis, and Chlamydia psittaci shown by two-dimensional gel electrophoresis.
abstract: Analysis by two-dimensional gel electrophoresis of the N-laurylsarkosinate(Sarkosyl)-insoluble envelope complexes of L-[35]S-cysteine-labeled elementary bodies of Chlamydia pneumoniae strain IOL-207, Chlamydia trachomatis serovar LGV2, D, and F, and Chlamydia psittaci strain 6BC showed differences in the molecular charges of chlamydial outer membrane proteins. The apparent isoelectric point (pI) of the major outer membrane protein of C. pneumoniae strain IOL-207 was 6.4, whereas the pI of the major outer membrane protein of the C. trachomatis and C. psittaci strains differed little from one another, ranging from 5.3 to 5.5. The 60-kDa cysteine-rich protein of C. pneumoniae was the only 60-kDa chlamydial protein with a pI value (5.9) more acidic than that of the corresponding major outer membrane protein. As a general rule, the charges of both the 60-kDa and the low-molecular-mass (12-15 kDa) cysteine-rich proteins were widely variable, depending on the strain. However, in each individual strain, the variation of the charge of the 60-kDa protein had a compensatory change in the low-molecular-mass cysteine-rich protein.
authors.affiliation: Institute of Microbiology, University of Bologna, St. Orsola Hospital, Via Massarenti 9, I-40138 Bologna, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Moroni
authors.forename: G
authors.initials: G
authors.lastname: Pavan
authors.forename: M
authors.initials: M
authors.lastname: Donati
authors.forename: R
authors.initials: R
authors.lastname: Cevenini
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry*; D002692:Chlamydia trachomatis / Q000737:chemistry*; D016993:Chlamydophila pneumoniae / Q000737:chemistry*; D002691:Chlamydophila psittaci / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D007526:Isoelectric Point; D008970:Molecular Weight
chemical_list: D001425:Bacterial Outer Membrane Proteins