title: Spectroscopic study of the interaction of aluminum ions with human transferrin.
abstract: Transferrin is the plasma protein responsible for transporting Fe3+ from the absorption to the utilization site. Interactions of apo- and holo-transferrin with Al3+ were studied by circular dichroism (CD), UV-visible, and fluorescence spectrometry. Binding of Al3+ to both metal-ion binding sites of apo-transferrin was confirmed by fluorescence studies. No interaction of Al3+ with holo-transferrin was observed, indicating that Al3+ cannot displace Fe3+ under the experimental conditions employed. An increase in tryptophan fluorescence (lambda max at 330 nm) by excitation at either 280 or 295 nm was observed after Al3+ interaction with apo-transferrin. There was no shift in wavelength of the fluorescence band of apo-transferrin after interaction with Al3+, but the intensity did increase. Since excitation at 295 nm is specific for tryptophan residues, tryptophan but not tyrosine must be responsible for the change in fluorescence intensity. Decreased fluorescence is the result of Fe3+ binding to apo-transferrin. The CD spectrum of apo-transferrin was slightly affected in the far UV by Al3+ binding, but a salient change was noted in the near UV at approximately 288 nm where tyrosine and tryptophan absorb. It is concluded that a small conformational change in the protein was induced by Al3+ binding to apo-transferrin.
authors.affiliation: Department of Environmental Health and Toxicology, School of Public Health, State University of New York at Albany, USA.
authors.forename: S
authors.initials: S
authors.lastname: Tang
authors.forename: R
authors.initials: R
authors.lastname: MacColl
authors.forename: P J
authors.initials: PJ
authors.lastname: Parsons
mesh_terms: D000535:Aluminum / Q000378:metabolism* / Q000494:pharmacology; D001059:Apoproteins / Q000737:chemistry / Q000378:metabolism*; D002942:Circular Dichroism; D006801:Humans; D011485:Protein Binding; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D013050:Spectrometry, Fluorescence; D013053:Spectrophotometry; D014168:Transferrin / Q000737:chemistry / Q000378:metabolism*
chemical_list: D001059:Apoproteins; D014168:Transferrin; C028284:apotransferrin; D000535:Aluminum