In vivo response of mature T cells to Mlsa antigens. Long-term progeny of dividing cells include cells with a naive phenotype.
To characterize T cells surviving elimination following strong in vivo responses, bromodeoxyuridine (BrdU) was used to mark the V beta 6+ CD4+ T cells proliferating after exposure to MLsa superantigens. Though a portion of the surviving V beta 6+ cells failed to label with BrdU, many were BrdU+, indicating that some proliferating cells escape elimination. Both the portion of V beta 6+ cells responding to MLs Ag in vivo and the number of responding cells that escaped elimination were influenced by the MHC haplotype; weaker responses correlated with enhanced survival of BrdU+ V beta 6+ cells. As expected, some of these cells expressed cell surface markers generally associated with an activated/memory phenotype. Significantly, however, a large proportion of BrdU+ V beta 6+ cells were phenotypically indistinguishable from naive cells.
Hayden
K A
KA
Department of Immunology, Scripps Research Institute, La Jolla, CA 92037, USA.
Tough
D F
DF
Webb
S R
SR
D000818:Animals; D000954:Antigens, Surface / Q000032:analysis; D015415:Biomarkers / Q000032:analysis; D001973:Bromodeoxyuridine / Q000494:pharmacology; D002455:Cell Division / Q000276:immunology; D002470:Cell Survival / Q000276:immunology; D006183:H-2 Antigens / Q000276:immunology; D016130:Immunophenotyping; D008213:Lymphocyte Activation; D008212:Lymphocyte Depletion; D051379:Mice; D008806:Mice, Inbred AKR; D008810:Mice, Inbred C57BL; D016747:Minor Lymphocyte Stimulatory Antigens / Q000008:administration & dosage / Q000494:pharmacology*; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000276:immunology; D013601:T-Lymphocytes / Q000166:cytology* / Q000276:immunology* / Q000637:transplantation
D000954:Antigens, Surface; D015415:Biomarkers; D006183:H-2 Antigens; D016747:Minor Lymphocyte Stimulatory Antigens; D016693:Receptors, Antigen, T-Cell, alpha-beta; D001973:Bromodeoxyuridine