On the mechanism by which 6-ketocholestanol protects mitochondria against uncoupling-induced Ca2+ efflux.
This work shows that 6-ketocholestanol (kCh) inhibits the effect of carbonyl cyanide-m-chlorophenyl hydrazone (CCP) on mitochondrial Ca2+ efflux. Such an effect proved to be caused by diminution of membrane fluidity, therefore, it is affected by the incubation temperature. Furthermore, kCh reversed CCP-induced Ca2+ efflux depending on the accumulation of phosphate. It is also shown that kCh enhances the effect of carboxyatractyloside on membrane permeability transition.
Chávez
E
E
Departamento de Bioquimica, Instituto Nacional de Cardiología, Mexico.
Moreno-Sánchez
R
R
Zazueta
C
C
Cuéllar
A
A
Ramirez
J
J
Reyes-Vivas
H
H
Bravo
C
C
Rodríguez-Enríquez
S
S
D000818:Animals; D001278:Atractyloside / Q000031:analogs & derivatives / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000037:antagonists & inhibitors / Q000494:pharmacology; D007425:Intracellular Membranes / Q000187:drug effects; D007653:Ketocholesterols / Q000494:pharmacology*; D007668:Kidney / Q000378:metabolism; D008560:Membrane Fluidity / Q000187:drug effects; D008928:Mitochondria / Q000378:metabolism*; D010539:Permeability / Q000187:drug effects; D051381:Rats; D014812:Vitamin K / Q000494:pharmacology
D007653:Ketocholesterols; D014812:Vitamin K; D001278:Atractyloside; C063833:6-ketocholestanol; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone; C003853:carboxyatractyloside; D002118:Calcium