Blockers of volume-activated Cl- currents inhibit endothelial cell proliferation.
Volume-activated Cl- currents (ICl,vol) and cell growth have been measured in cultured endothelial cells from bovine pulmonary artery (CPAE) in the absence and presence of compounds which block these currents. The anti-oestrogen drug tamoxifen, which efficiently arrests the growth of breast cancer cells (1), inhibits both ICl,vol and cell proliferation with IC50 of 3.8 and 4.8 micromol/l respectively.NPPB and quinine, which also block ICl,vol, inhibit the growth of CPAE cells as well. Current and cell growth were closely correlated under all these conditions. We conclude that ICl,vol might be involved in the control of endothelial cell growth and thus might be important for the modulation of vascularisation and vascular remodelling.
Voets
T
T
KU Leuven, Laboratorium voor Fysiologie, Campus Gasthuisberg, Herestraat, B-3000 Leuven, Belgium.
Szücs
G
G
Droogmans
G
G
Nilius
B
B
D000818:Animals; D002417:Cattle; D002452:Cell Count; D002455:Cell Division / Q000187:drug effects; D048429:Cell Size / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D018118:Chloride Channels / Q000187:drug effects*; D004594:Electrophysiology; D004730:Endothelium, Vascular / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D008564:Membrane Potentials / Q000187:drug effects; D009125:Muscle Relaxants, Central / Q000494:pharmacology; D009578:Nitrobenzenes / Q000494:pharmacology; D018408:Patch-Clamp Techniques; D011651:Pulmonary Artery / Q000166:cytology / Q000187:drug effects; D011803:Quinine / Q000494:pharmacology; D013629:Tamoxifen / Q000494:pharmacology
D018118:Chloride Channels; D009125:Muscle Relaxants, Central; D009578:Nitrobenzenes; D013629:Tamoxifen; C047633:5-(4-nitrophenyl)-2,4-pentadienal; D011803:Quinine