Microinjection of fluorescence dye in a plant cell and its intercellular translocation using a multi-channel microelectrode system.
Lucifer yellow CH dye (LY) was electrophoretically injected into a cell of a cultured tobacco (BY-2) using a multi-channel microelectrode (MME). Pulsing electricx potential (pulse width:90 ms, pulse interval: 80 ms) was applied between the potential-applying (PA) electrodes of both MMESs . The PA-electrode positioned in the LY-bearing cell was set negative against the other PA-electrode. When the pulse height was 340 V, intercellular translocation of LY from the LY-bearing cell to the neighboring cell was observed within 10 s.
Saito
M
M
Department of Biotechnology, Tokyo University of Agriculture and Technology, Japan.
Ohi
A
A
Matsuoka
H
H
D002478:Cells, Cultured; D005456:Fluorescent Dyes / Q000737:chemistry*; D007546:Isoquinolines / Q000737:chemistry; D008839:Microelectrodes; D010944:Plants / Q000737:chemistry*; D015398:Signal Transduction
D005456:Fluorescent Dyes; D007546:Isoquinolines; C017475:lucifer yellow