The transverse location of the fluorescent probe trans-parinaric acid in lipid bilayers.
The transverse location of trans-parinaric acid in spherical vesicles made up from dipalmitoylphosphatidylcholine has been investigated by the differential quenching of the probe fluorescence by 5- and 16-doxylstearic acid derivatives. The quenching data are interpreted in terms of a local fluorophore concentration factor. In this way it was found that the polyene of t-PnA is located within the inner part of the bilayer (presumably aligned with the bilayer lipids), both in the gel and in the liquid crystalline phases.
Castanho
M
M
Centro de Química-Física Molecular, Instituto Superior Técnico, Lisboa, Portugal.
Prieto
M
M
Acuña
A U
AU
D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry*; D003497:Cyclic N-Oxides; D005231:Fatty Acids, Unsaturated / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D008051:Lipid Bilayers / Q000737:chemistry*; D013113:Spin Labels
D003497:Cyclic N-Oxides; D005231:Fatty Acids, Unsaturated; D005456:Fluorescent Dyes; D008051:Lipid Bilayers; D013113:Spin Labels; D015060:1,2-Dipalmitoylphosphatidylcholine; C005072:5-doxylstearic acid; C032363:16-nitroxystearic acid; C013641:parinaric acid