Prophylactic and therapeutic efficacies of poly(IC.LC) against respiratory influenza A virus infection in mice.
Polyriboinosinic-polyribocytidylic acid [poly(IC.LC)] was evaluated for its prophylactic and therapeutic efficacies against respiratory influenza A virus infection in mice. Two doses of poly(IC.LC) (1 mg/kg of body weight per dose) administered intranasally within 12 days prior to infection with 10 50% lethal doses of mouse-adapted influenza A/PR/8 virus fully protected the mice against the infection. Determination of virus titers by hemagglutination and plaque assays showed more than a 2-log10 decrease in virus titers in lung homogenates of pretreated mice compared with those in the lungs of the nonpretreated group. Treatment of infected mice with poly(IC.LC) resulted in a modest (40%) survival rate. These results suggest that poly(IC.LC) provides a highly effective prophylaxis against respiratory influenza A virus infection in mice.
Wong
J P
JP
Medical Countermeasures Section, Defence Research Establishment Suffield, Ralston, Alberta, Canada.
Saravolac
E G
EG
Sabuda
D
D
Levy
H B
HB
Kende
M
M
D000281:Administration, Intranasal; D000818:Animals; D000998:Antiviral Agents / Q000627:therapeutic use*; D002266:Carboxymethylcellulose Sodium / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000627:therapeutic use; D009980:Influenza A virus; D007274:Injections, Intraperitoneal; D007369:Interferon Inducers / Q000008:administration & dosage / Q000627:therapeutic use*; D007372:Interferons / Q000502:physiology; D008168:Lung / Q000821:virology; D051379:Mice; D009976:Orthomyxoviridae Infections / Q000188:drug therapy* / Q000517:prevention & control / Q000821:virology; D011070:Poly I-C / Q000008:administration & dosage / Q000627:therapeutic use*; D011107:Polylysine / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000627:therapeutic use; D010948:Viral Plaque Assay
D000998:Antiviral Agents; D007369:Interferon Inducers; D011107:Polylysine; C019531:poly ICLC; D007372:Interferons; D002266:Carboxymethylcellulose Sodium; D011070:Poly I-C