title: Reference intervals for plasma apolipoprotein A-1 determined with a standardized commercial immunoturbidimetric assay: results from the Framingham Offspring Study.
abstract: We have evaluated a commercially available, standardized immunoturbidimetric assay of apolipoprotein (apo) A-I, the major protein constituent of high-density lipoproteins (HDL). We determined the reference ranges of plasma apo A-I concentration for white men and women and related these values to the risk of coronary heart disease (CHD). The mean between-run CV for this assay was 2.9%. As determined with individuals participating cycle 4 of the Framingham Offspring Study, the mean (+/- SD) apo A-I concentration was 13% lower in 1879 men (1.34 +/- 0.23 g/L) than in 1939 women (1.54 +/- 0.28 g/L) (P<0.001). An apo A-I concentration of 1.20 g/L roughly corresponded to the 25th percentile value in men and the 5th percentile in women, and subjects with a concentration below this value were significantly more likely to have CHD than subjects wit a higher concentration (P<0.001).
authors.affiliation: Jean Mayer USDA Human Nutrition Research Center on Aging at Tufts University, Boston, MA 02111, USA.
authors.forename: J
authors.initials: J
authors.lastname: Contois
authors.forename: J R
authors.initials: JR
authors.lastname: McNamara
authors.forename: C
authors.initials: C
authors.lastname: Lammi-Keefe
authors.forename: P W
authors.initials: PW
authors.lastname: Wilson
authors.forename: T
authors.initials: T
authors.lastname: Massov
authors.forename: E J
authors.initials: EJ
authors.lastname: Schaefer
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016632:Apolipoprotein A-I / Q000032:analysis*; D008076:Cholesterol, HDL / Q000097:blood; D003327:Coronary Disease / Q000097:blood; D005260:Female; D006801:Humans; D007118:Immunoassay / Q000706:statistics & numerical data; D008297:Male; D008875:Middle Aged; D009391:Nephelometry and Turbidimetry; D012016:Reference Values; D012307:Risk Factors; D012727:Sex Characteristics
chemical_list: D016632:Apolipoprotein A-I; D008076:Cholesterol, HDL