title: Report on the Sixth International Society of Blood Transfusion Platelet Serology Workshop.
abstract: BACKGROUND
  The Sixth International Society of Blood Transfusion Platelet Serology Workshop continued studies to identify methods to detect platelet-specific antigens and antibodies.
  
  
  METHODS
  The study was designed to meet three goals. The first was the establishment of antigen-typed platelet panels and determination of the correlation between serologic and DNA typing of platelet-specific antigens. The second goal was the determination of the proficiency of detecting platelet-specific antibodies by laboratory and by technique. The third goal was the identification of any platelet-specific antibodies present in uncharacterized (unknown) antisera.
  
  
  RESULTS
  For platelet-antigen typing, concordance between serologic testing and DNA techniques was 93 percent for oligonucleotide typing and 92 percent for allele-specific restriction site analysis. Agreement between these two was 98 percent. Individual laboratories correctly identified the antibodies contained in coded sera 79 +/- 17 percent of the time. The expected results were obtained from the modified antigen-capture enzyme-linked immunosorbent assay in 75 +/- 46 percent of instances, from the monoclonal antibody-specific immobilization of platelet antigens assay in 72 +/- 24 percent of instances, from the mixed passive hemagglutination assay in 71 +/- 13 percent, from radioimmunoprecipitation procedures in 67 +/- 47 percent, and from Western blot 34 +/- 40 percent. Seven (54%) of 13 antisera of unknown specificity were determined to contain clearly identifiable platelet-specific alloantibodies.
  
  
  CONCLUSIONS
  Concordant results were achieved by using either serologic or DNA techniques to identify platelet-specific antigens. Except for the significantly lower results found with Western blotting, all other platelet-specific antibody assays were comparable. Established serologic laboratories can identify and characterize plate-specific antibodies.
authors.affiliation: Platelet Immunology Laboratory, Puget Sound Blood Center, Seattle Washington, USA.
authors.forename: G
authors.initials: G
authors.lastname: Teramura
authors.forename: S J
authors.initials: SJ
authors.lastname: Slichter
mesh_terms: D000918:Antibody Specificity; D001792:Blood Platelets / Q000276:immunology*; D006801:Humans; D007518:Isoantibodies / Q000276:immunology*; D007519:Isoantigens / Q000276:immunology
chemical_list: D007518:Isoantibodies; D007519:Isoantigens