CD14 mediate endotoxin induction of nitric oxide synthase in cultured brain glial cells.
Here we show that lipopolysaccharide (LPS) induction of glial inducible nitric oxide synthase (iNOS) requires membrane (m) and soluble (s) forms of CD14. In glial cell cultures, an anti-rat CD14 monoclonal antibody detected CD14 protein in whole cells and cell lysates, and reduced LPS-dependent iNOS expression. Glial cells and normal brain tissue expressed CD14 mRNA, as revealed by isolation of a rat CD14 clone (rCD14) from an astrocyte cDNA library and RT-PCR analysis. Finally, serum the ED(50) of LPS required for glial iNOS expression, and antibodies against sCD14 blocked the potentiating effect of serum.
Galea
E
E
Division of Neurobiology, Department of Neuroscience and Neurology, Cornell University Medical College, New York 10021, USA.
Reis
D J
DJ
Fox
E S
ES
Xu
H
H
Feinstein
D L
DL
D000595:Amino Acid Sequence; D000818:Animals; D001253:Astrocytes / Q000201:enzymology*; D001483:Base Sequence; D002478:Cells, Cultured; D004790:Enzyme Induction; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D018950:Lipopolysaccharide Receptors / Q000235:genetics / Q000502:physiology*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008969:Molecular Sequence Data; D019001:Nitric Oxide Synthase / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats
D007106:Immune Sera; D018950:Lipopolysaccharide Receptors; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D019001:Nitric Oxide Synthase