[Determination of intestinal strontium absorption--assessment and validation of routinely manageable test procedures].
Intestinal strontium absorption has been discussed recently as an indirect measure for calcium uptake. Prerequisite for the clinical use of an oral strontium test is the availability of a reliable procedure including controlled strontium supply, sample pretreatment and analysis as well as the assessment of normal values. In the present study, a group of young females (n = 33; 24.0 +/- 2.7 y; BMI 21.5 +/- 1.9) received an oral dose of 2.27 mmol strontium in a standardized breakfast that contained 0.625 mmol calcium. Before and 220 min after the bolus serum strontium concentrations were determined by means of atomic absorption spectrophotometry (coefficient of variation: within day 4.8%, n = 10; day-to-day 9.5%, n = 8). The error of the method was 2.7%. Calculation of the fractional strontium absorption rate considered the respective distribution volume (extracellular fluid; either estimated using body weight or determined by means of bioimpedance analysis [BIA]). Average absorption rates were 13.3 +/- 3.1% and, considering BIA measurement 13.6 +/- 2.6%, respectively. Smoking, exercise and, use of oral contraceptives showed no effects. Our oral strontium test is characterized by excellent reliability, easy handling and low costs and, thus, is suitable for routine use.
Zittermann
A
A
Institut für Ernährungswissenschaft Universität Bonn.
Bierschbach
C
C
Giers
G
G
Hötzel
D
D
Stehle
P
P
D000284:Administration, Oral; D000328:Adult; D000704:Analysis of Variance; D001682:Biological Availability; D002118:Calcium / Q000378:metabolism; D005260:Female; D006801:Humans; D007275:Injections, Intravenous; D007408:Intestinal Absorption; D015203:Reproducibility of Results; D013054:Spectrophotometry, Atomic / Q000379:methods; D013324:Strontium / Q000008:administration & dosage / Q000097:blood / Q000493:pharmacokinetics*
D002118:Calcium; D013324:Strontium