Acetylcholine release from dissociated striatal cells.
To study the regulation of striatal acetylcholine (ACH) release, adult male rat striata were dissociated and incubated with 3H-choline to synthesize 3H-ACH. Fractional 3H-ACH efflux per min during continuous perifusion was: (1) tightly regulated; (2) dependent on calcium influx; (3) stimulated by 10 mM K+ and 1 mM glutamate; and (4) comparable to ACH release detected by HPLC. Thus, acutely dissociated striata exhibit calcium-sensitive, voltage-dependent secretion of 3H-ACH and direct receptor-mediated stimulation of release through the glutamate receptor family. This new approach toward cholinergic secretory physiology will help clarify complex striatal circuitry.
Login
I S
IS
Department of Neurology, University of Virginia Health Sciences Center, Charlottesville 22908, USA.
Borland
K
K
Harrison
M B
MB
Ragozzino
M E
ME
Gold
P E
PE
D000109:Acetylcholine / Q000378:metabolism*; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D003342:Corpus Striatum / Q000166:cytology / Q000378:metabolism*; D018698:Glutamic Acid / Q000494:pharmacology; D008297:Male; D011188:Potassium / Q000494:pharmacology; D051381:Rats
D018698:Glutamic Acid; D000109:Acetylcholine; D011188:Potassium; D002118:Calcium