Characterization of an in vitro transcription system from rinderpest virus.
An in vitro transcription system for rinderpest virus (RPV) is described. Ribonucleoprotein complexes isolated from RPV-infected Vero cells, human lung carcinoma cells, or detergent-disrupted purified virions synthesized authentic RPV mRNAs for the N, P, M. F and H genes as identified by dot blot hybridization analysis with individual cDNA clones. The relative abundance of the mRNAs synthesized in vitro decreased from the 3' end of the genome to the 5' end, very similar to that observed with measles virus transcription in vitro. The transcription by purified virions was stimulated three-fold by the addition of infected human lung carcinoma cell lysate, demonstrating the involvement of host factor(s) in mRNA synthesis.
Ghosh
A
A
Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore.
Joshi
V D
VD
Shaila
M S
MS
D000818:Animals; D002277:Carcinoma; D002522:Chlorocebus aethiops; D006801:Humans; D008175:Lung Neoplasms; D008970:Molecular Weight; D012367:RNA, Viral / Q000096:biosynthesis*; D012261:Ribonucleoproteins / Q000032:analysis / Q000096:biosynthesis*; D012302:Rinderpest virus / Q000378:metabolism* / Q000502:physiology; D014158:Transcription, Genetic / Q000502:physiology*; D014407:Tumor Cells, Cultured; D014709:Vero Cells; D014764:Viral Proteins / Q000032:analysis / Q000096:biosynthesis
D012367:RNA, Viral; D012261:Ribonucleoproteins; D014764:Viral Proteins