Endothelin stimulates protein phosphorylation in blood-brain barrier.
The vasoactive neuropeptide endothelin stimulated phosphorylation of specific proteins in rat brain microvessels. Major effects were observed with endothelin-1. These effects were dose- and time-dependent. Among substrates for kinase actions, the 17.5 KDa and 80 KDa proteins were mainly affected by endothelin-1. It was demonstrated that the 80 KDa protein affected corresponds to MARCKS (myristoylated alanine-rich C kinase substrate), a specific substrate for protein kinase C action.
Catalán
R E
RE
Departamento de Biología Molecular, Centro de Biología Molecular "Severo Ochoa", Universidad Autónoma de Madrid, Spain.
Martínez
A M
AM
Aragonés
M D
MD
Hernández
F
F
Diáz
G
G
D000818:Animals; D001812:Blood-Brain Barrier; D001921:Brain / Q000098:blood supply; D002560:Cerebrovascular Circulation; D004591:Electrophoresis, Polyacrylamide Gel; D016232:Endothelins / Q000494:pharmacology*; D047908:Intracellular Signaling Peptides and Proteins; D008297:Male; D008565:Membrane Proteins; D008833:Microcirculation / Q000378:metabolism*; D008970:Molecular Weight; D000076250:Myristoylated Alanine-Rich C Kinase Substrate; D010710:Phosphates / Q000378:metabolism; D010750:Phosphoproteins / Q000302:isolation & purification / Q000378:metabolism; D010761:Phosphorus Radioisotopes; D010766:Phosphorylation; D011493:Protein Kinase C / Q000378:metabolism; D011506:Proteins / Q000302:isolation & purification / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013379:Substrate Specificity
D016232:Endothelins; D047908:Intracellular Signaling Peptides and Proteins; C000619712:Marcks protein, rat; D008565:Membrane Proteins; D010710:Phosphates; D010750:Phosphoproteins; D010761:Phosphorus Radioisotopes; D011506:Proteins; D000076250:Myristoylated Alanine-Rich C Kinase Substrate; D011493:Protein Kinase C