title: Two karyotypically unrelated clones with 5q- and 20q- in a primary myelodysplastic syndrome patient evolving into acute nonlymphocytic leukemia.
abstract: We describe a patient with primary myelodysplastic syndrome (MDS) evolving into acute nonlymphocytic leukemia (ANLL) who had two cytogenetically unrelated abnormal clones. A 68-year-old man presented with refractory anemia with excess of blasts (RAEB) and developed overt ANLL. Two cytogenetically independent clones, one with 5q- and the other with 20q-, were observed when the patient developed ANLL. The clones carrying both 5q- and 20q- were not detected. Leukemic blast cells were positive for peroxidase, naphtol ASD chloroacetate esterase, CD13, CD33, CD34 and HLA-DR, but negative for alpha-naphthyl butyrate esterase, CD14, CD10, CD19, CD20, CD1, CD2, CD3, CD5 and CD7. Although there have been a few reports describing the presence of multiple cytogenetically unrelated clones in one patient with MDS, this is the first case report that the 5q- and 20q- anomalies are derived from independent clones.
authors.affiliation: Department of Internal Medicine III, Akita University School of Medicine, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Hirokawa
authors.forename: I
authors.initials: I
authors.lastname: Miura
authors.forename: A B
authors.initials: AB
authors.lastname: Miura
mesh_terms: D000368:Aged; D000754:Anemia, Refractory, with Excess of Blasts / Q000235:genetics* / Q000473:pathology; D014408:Biomarkers, Tumor; D002872:Chromosome Deletion; D002890:Chromosomes, Human, Pair 20 / Q000648:ultrastructure*; D002895:Chromosomes, Human, Pair 5 / Q000648:ultrastructure*; D018450:Disease Progression; D017809:Fatal Outcome; D006801:Humans; D016130:Immunophenotyping; D007621:Karyotyping; D015470:Leukemia, Myeloid, Acute / Q000235:genetics*; D008297:Male; D014411:Neoplastic Stem Cells / Q000737:chemistry / Q000473:pathology*
chemical_list: D014408:Biomarkers, Tumor