Sequence conservation within the major capsid protein of human papillomavirus (HPV) type 18 and formation of HPV-18 virus-like particles in Saccharomyces cerevisiae.
The major capsid protein L1 of human papillomaviruses (HPVs) has been identified as a promising candidate antigen for a prophylactic HPV vaccine. Since amino acid sequence heterogeneity has been demonstrated for the L1 genes within individual HPV types, nucleotide sequences of L1 were determined from six HPV-18 clinical isolates and the cervical carcinoma cell line SW756 and compared to the published HPV-18 prototype sequence. The sequences were almost identical between the clinical isolates and SW756 but differed markedly from the published prototype sequence. Resequencing the prototype HPV-18 revealed that these differences were due to sequencing artifacts of the prototype HPV-18 sequence archived in GenBank. Thus, the HPV-18 L1 genes seem to display a very high level of sequence conservation. The HPV-18 L1 gene derived from SW756 was expressed in Saccharomyces cerevisiae and self-assembly of the L1 protein into virus-like particles was demonstrated.
Hofmann
K J
KJ
Department of Virus and Cell Biology, Merck Research Laboratories, West Point, Pennsylvania 19486, USA.
Neeper
M P
MP
Markus
H Z
HZ
Brown
D R
DR
Müller
M
M
Jansen
K U
KU
D001483:Base Sequence; D002213:Capsid / Q000235:genetics* / Q000648:ultrastructure; D003001:Cloning, Molecular; D017124:Conserved Sequence; D004279:DNA, Viral / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D027383:Papillomaviridae / Q000235:genetics* / Q000302:isolation & purification / Q000648:ultrastructure; D011994:Recombinant Proteins / Q000235:genetics / Q000648:ultrastructure; D012441:Saccharomyces cerevisiae; D014407:Tumor Cells, Cultured
D004279:DNA, Viral; D011994:Recombinant Proteins