Truncated form of pro-acetylcholine receptor-inducing activity (ARIA) induces AChR alpha-subunit but not AChE transcripts in cultured chick myotubes.
Acetylcholine receptor-inducing activity (ARIA) is a glycoprotein initially purified from chick brain based on its ability to increase the synthesis of acetylcholine receptor (AChR). We used reverse transcription-polymerase chain reaction (RT-PCR) to obtain a partial pro-ARIA cDNA clone from methonine-1 to serine-358 including the full functional sequence of ARIA. Northern blot analysis of mRNAs from the embryonic chick brain and muscle showed a transcript with a size of approximately 7.5 kb. The cloned cDNA was subcloned into an eukaryotic expression vector and stably transfected into human embryonic kidney 293 cells. The conditioned medium of the transfected cells was found to increase the level of transcript encoding for the alpha-subunit of AChR by approximately 4.4-fold, but not for acetylcholinesterase (AChE), in the cultured chick myotubes.
Pun
S
S
Department of Biology, Hong Kong University of Science and Technology, Kowloon, Hong Kong.
Tsim
K W
KW
D000110:Acetylcholinesterase / Q000096:biosynthesis*; D000818:Animals; D030161:Avian Proteins; D001483:Base Sequence; D015152:Blotting, Northern; D002460:Cell Line; D002642:Chick Embryo; D017077:Culture Media, Conditioned; D018076:DNA, Complementary / Q000096:biosynthesis; D006801:Humans; D008969:Molecular Sequence Data; D009132:Muscles / Q000187:drug effects / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000494:pharmacology*; D020890:Neuregulin-1; D009469:Neuromuscular Junction / Q000187:drug effects / Q000378:metabolism; D010446:Peptide Fragments / Q000494:pharmacology*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis; D011950:Receptors, Cholinergic / Q000096:biosynthesis* / Q000235:genetics; D014162:Transfection / Q000235:genetics
D030161:Avian Proteins; D017077:Culture Media, Conditioned; D018076:DNA, Complementary; C490582:NRG1 protein, Gallus gallus; C094131:NRG1 protein, human; D009419:Nerve Tissue Proteins; D020890:Neuregulin-1; D010446:Peptide Fragments; D012333:RNA, Messenger; D011950:Receptors, Cholinergic; D000110:Acetylcholinesterase