Heterogeneous calcium flux in peripheral T cell subsets revealed by five-color flow cytometry using log-ratio circuitry.
Calcium flux measurements of different subpopulations of cells by flow cytometry are important in understanding complex interactions in the immune system. This paper discusses the use of the difference of Log signals as a preferred method for obtaining this information simultaneously with other immunofluorescence parameters. We describe simple modifications to a commercial instrument that enables the measurement of calcium flux in addition to three immunofluorescence parameters. Finally, we show an application of this technique to measuring calcium flux of T cell subsets in human blood. We show that different subsets of peripheral CD4 T cells have significantly different capabilities to flux calcium after CD3 stimulation. These differences are related to the functional capacities of the cells within these subsets.
Roederer
M
M
Department of Genetics, Stanford University School of Medicine, California 94305-5125, USA.
Bigos
M
M
Nozaki
T
T
Stovel
R T
RT
Parks
D R
DR
Herzenberg
L A
LA
D015703:Antigens, CD / Q000032:analysis*; D015704:CD4 Antigens / Q000032:analysis; D002118:Calcium / Q000378:metabolism*; D002460:Cell Line; D004581:Electronics; D005434:Flow Cytometry / Q000295:instrumentation* / Q000379:methods*; D005456:Fluorescent Dyes; D006801:Humans; D007211:Indoles; D017493:Leukocyte Common Antigens / Q000032:analysis; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D016176:T-Lymphocyte Subsets / Q000166:cytology* / Q000276:immunology / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D015703:Antigens, CD; D015704:CD4 Antigens; D005456:Fluorescent Dyes; D007211:Indoles; D017493:Leukocyte Common Antigens; C048960:indo-1; D002118:Calcium