[Morphological evaluation of intercellular junctions in the ovary of chick embryos: action of steroid and gonadotropic hormones in vitro].
Variations of the intercellular junctions of the germ and epithelial cells of the ovarian epithelium produced by gonadotropic and esteroid hormones were determined on ovaries of the chick embryo at 7 days of development. Explants of right and left ovaries were cultured without (control) or with hormones eddition (experimental) for 4 days. Cultures were processed for their ultrastructural study. In both control ovaries the union complexes were similar to those identified in ovo. Under the action of 17 beta-estradiol, an increase and a greater development of adherens junctions was observed in the left ovary; in the right ovary, adherens junctions diminished by action of 17 beta-estradiol. The response of the left ovary to progesterone and testosterone was similar to that seen with the estrogen. No changes were observed in the right gonad. A disminution of intercellular junctions was produced in both ovaries under the action of FSH. The changes produced by LH and hCG were similar to those find in the left ovary with the estrogen, consisting of an increase of the union complexes, mainly of the adherens type. These results indicate that steroid and gonadotropic hormones act by modifying intercellular junctions and would participate in the processes of growth and atrophy that occur in the ovaries of the chick embryo.
Avila
R E
RE
Ila Cátedra de Histología y Genética, Facultad de Ciencias Médicas, UNC, Córdoba, Argentina.
Samar
M E
ME
de Fabro
S P
SP
D000818:Animals; D002642:Chick Embryo; D005260:Female; D012739:Gonadal Steroid Hormones / Q000494:pharmacology*; D006062:Gonadotropins / Q000494:pharmacology*; D066298:In Vitro Techniques; D007365:Intercellular Junctions / Q000187:drug effects / Q000648:ultrastructure*; D010053:Ovary / Q000187:drug effects / Q000196:embryology* / Q000648:ultrastructure*
D012739:Gonadal Steroid Hormones; D006062:Gonadotropins