Correlation between levels of delta protein kinase C and resistance to differentiation in murine erythroleukemia cells.
It has been demonstrated that the level of delta protein kinase C is inversely correlated to the responsiveness of murine erythroleukemia cells to chemical induction to terminal erythroid differentiation. In these cells, deltaPKC is largely present in a membrane associated form, and thus in a constitutively active state, a condition which characterizes the undifferentiated phenotype. Accordingly, commitment to cell differentiation has been shown to be preceded by down regulation of deltaPKC, a process significantly accelerated and induced to almost completion by the differentiation enhancing factor (DEF) in a dose dependent manner. The present results provide a better understanding of the role of deltaPKC in characterizing the undifferentiated MEL cell phenotype and suggest a relationship between the acceleration in the rate of differentiation induced by DEF and the down regulation of this kinase form.
Patrone
M
M
Institute of Biological Chemistry, University of Genova, Italy.
Pessino
A
A
Passalacqua
M
M
Sparatore
B
B
Melloni
E
E
Pontremoli
S
S
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D015536:Down-Regulation; D024243:HMGB1 Protein; D006651:Histocytochemistry; D007527:Isoenzymes / Q000378:metabolism*; D004915:Leukemia, Erythroblastic, Acute / Q000201:enzymology* / Q000473:pathology*; D051379:Mice; D009363:Neoplasm Proteins / Q000494:pharmacology; D010641:Phenotype; D011493:Protein Kinase C / Q000378:metabolism*; D051745:Protein Kinase C-delta; D014407:Tumor Cells, Cultured
D024243:HMGB1 Protein; D007527:Isoenzymes; D009363:Neoplasm Proteins; C497259:Prkcd protein, mouse; D011493:Protein Kinase C; D051745:Protein Kinase C-delta