Microbial production of specifically ring-13C-labelled 4-hydroxybenzoic acid.
When transformed with a recombinant vector carrying the ubiC gene (encoding chorismate pyruvate-lyase, EC 4.1.3.27) the triple mutant (Phe-, Trp-, Tyr-) Klebsiella pneumoniae 62-1 excretes 4-hydroxybenzoic acid instead of chorismic acid. The recombinant strain can be used to produce in high yield specifically ring-labelled 4-hydroxybenzoic acid from isotopically labelled glucose.
Müller
R
R
Institut für Pharmazeutische Biologie, Rheinische Friedrich-Wilhelms-Universität Bonn, Germany.
Wagener
A
A
Schmidt
K
K
Leistner
E
E
D000878:Anthranilate Synthase / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D002247:Carbon Isotopes; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D005822:Genetic Vectors; D005947:Glucose / Q000737:chemistry; D007553:Isotope Labeling; D007711:Klebsiella pneumoniae / Q000378:metabolism*; D008969:Molecular Sequence Data; D007652:Oxo-Acid-Lyases / Q000235:genetics / Q000378:metabolism*; D010226:Parabens / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D014169:Transformation, Bacterial
D001426:Bacterial Proteins; D002247:Carbon Isotopes; D010226:Parabens; D011994:Recombinant Proteins; D007652:Oxo-Acid-Lyases; C075960:chorismate pyruvate lyase; D000878:Anthranilate Synthase; D005947:Glucose; C038193:4-hydroxybenzoic acid