Undetectable expression of genomic progesterone receptor in human spermatozoa.
The expression of genomic progesterone receptor in human ejaculated spermatozoa was investigated. Spermatozoa from 10 fertile donors who exhibited normal semen parameters were analysed. Indirect immunofluorescence and an enzyme immunoassay using monoclonal antibodies against genomic progesterone receptor were used. Different types of spermatozoa were studied: fresh, post-swim-up (migrated), capacitated and post-artificial induction of the acrosome reaction by calcium ionophore A23187. Progestin receptor-rich T47D human breast cancer cells were used as a positive control, and progestin receptor-poor MDA-MB-231 human breast carcinoma cells were used as a negative control. Genomic progesterone receptor was not detected in fresh, migrated, capacitated and post-acrosome reaction induction human spermatozoa and MDA-MB-231 cells by either indirect immunofluorescence or enzyme immunoassay. However, in T47D cells a mean concentration of 1043.2 +/- 125.2 fmol genomic progesterone receptor/mg protein was observed by enzyme immunoassay, and indirect immunofluorescence results were positive using both flow cytometry and fluorescence microscopy. These findings suggest that the effect of progesterone on human spermatozoa is not mediated by genomic progesterone receptor.
Castilla
J A
JA
Servicio de Análisis Clínicos, Centro Materno-Infantil, Hospiktal Virgen de las Nieves, Granada, Spain.
Gil
T
T
Molina
J
J
Hortas
M L
ML
Rodriguez
F
F
Torres-Muñoz
J
J
Vergara
F
F
Herruzo
A J
AJ
D000911:Antibodies, Monoclonal; D019084:Fluorescent Antibody Technique, Indirect; D016678:Genome; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D011980:Receptors, Progesterone / Q000235:genetics* / Q000378:metabolism*; D013094:Spermatozoa / Q000378:metabolism*; D013194:Staining and Labeling
D000911:Antibodies, Monoclonal; D011980:Receptors, Progesterone