The identification of the neonatal NBXFO hybridoma cell and its mediator.
The neonatal spleen:myeloma hybridoma cell, NBXFO, with immunosuppressive properties supported rodent hematopoietic colony formation. We identified this hybridoma to be an undifferentiated fibroblast that produced macrophage colony-stimulating factor (M-CSF). The bone marrow cells that grew in the presence of the NBXFO supernate were macrophages and were immunosuppressive towards lymphocytes. Neutralizing anti-M-CSF antibody partially inhibited the actions of the neonatal splenic suppressor cells. Neonatal splenocytes, but not the other parental cell line, FO, induced macrophage colony formation, possessed surface-associated M-CSF, and possessed M-CSF-specific transcripts. Therefore, we believe that the M-CSF-producing phenotype was contributed by a fibroblastic stromal cell and that these stromal cells could be responsible for the in situ generation of neonatal splenic suppressor cells.
Jadus
M R
MR
Department of Laboratory Service, Veterans Affairs Medical Center, Long Beach, CA 90822, USA.
Irwin
M R
MR
Barton
B E
BE
Irwin
M C
MC
Wepsic
H T
HT
D000818:Animals; D000831:Animals, Newborn / Q000276:immunology*; D006825:Hybridomas / Q000276:immunology* / Q000378:metabolism; D007797:Laminin / Q000032:analysis; D016173:Macrophage Colony-Stimulating Factor / Q000032:analysis / Q000096:biosynthesis; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D051381:Rats; D013154:Spleen / Q000276:immunology*
D007797:Laminin; D016173:Macrophage Colony-Stimulating Factor