Volume-sensitive chloride channels in the primary culture cells of human cervical carcinoma.
Previous study shows volume-sensitive chloride currents are induced by hypotonicity in human cervical cancer cell lines, but not in normal cervical epithelium. To ascertain whether the preferential activation of these channels in cancer cell lines could be similarly and directly detected in cervical cancer tissues, we studied volume-sensitive chloride channels on the primary culture cells of invasive cervical carcinoma using the whole-cell patch-clamp technique. The process of regulatory volume decrease (RVD) was also studied using electronic cell sizing to measure cell volume. Results demonstrate that, in these cultured cells, RVD was mediated in part by chloride loss through the volume-sensitive Cl- channels. A small background current with a slope conductance of 0.32 +/- 0.07 nS/pF at +30 mV (n=60 cells from 10 different samples) was observed. Hypotonicity induced a fast activating and outward rectifying current which was reversed at about 0 mV, and the slope conductance at +30 mV was increased by 10-fold to 3.62 +/- 0.62 nS/pF. These effects were readily reversed by returning the cells to isotonic medium. Moreover, DIDS, NPPB, and 1,9-dideoxyforskolin, reversibly abolished the volume-sensitive Cl- currents. The EC50 required for the inhibitory effect of DIDS, NPPB and 1,9-dideoxyforskolin was 150, 120, and 50 microM, respectively. Volume-sensitive Cl- channels were ubiquitously expressed in cultured cells from 10 samples of different cancer stages, histopathologic types, and state of HPV DNA positivity. Interestingly, similar outward rectifying chloride currents were activated by intracellular 300 microM GTP gamma S. It is proposed that this Cl- conductance may play an important role leading to RVD in human cervical cancer.
Shen
M R
MR
Department of Obstetrics and Gynecology, National Cheng Kung University Medical College, Tainan.
Wu
S N
SN
Chou
C Y
CY
D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid / Q000494:pharmacology; D000230:Adenocarcinoma / Q000473:pathology / Q000503:physiopathology* / Q000821:virology; D001483:Base Sequence; D002294:Carcinoma, Squamous Cell / Q000473:pathology / Q000503:physiopathology* / Q000821:virology; D002462:Cell Membrane / Q000502:physiology; D002586:Cesium / Q000494:pharmacology; D018118:Chloride Channels / Q000037:antagonists & inhibitors / Q000187:drug effects / Q000502:physiology*; D002712:Chlorides / Q000494:pharmacology; D017931:DNA Primers; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D016679:Genome, Viral; D016244:Guanosine 5'-O-(3-Thiotriphosphate) / Q000494:pharmacology; D006801:Humans; D007700:Kinetics; D008564:Membrane Potentials / Q000502:physiology; D008969:Molecular Sequence Data; D009367:Neoplasm Staging; D027383:Papillomaviridae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D018408:Patch-Clamp Techniques; D016133:Polymerase Chain Reaction; D014407:Tumor Cells, Cultured; D002583:Uterine Cervical Neoplasms / Q000473:pathology / Q000503:physiopathology* / Q000821:virology
D018118:Chloride Channels; D002712:Chlorides; D017931:DNA Primers; D004279:DNA, Viral; D002586:Cesium; D016244:Guanosine 5'-O-(3-Thiotriphosphate); C028019:cesium chloride; D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid