[Expression of a gene encoding the porcine rotavirus VP7 capsid using a nondefective recombinant adenoviral genome].
A nondefective recombinant human adenovirus 5 (Ad5) carrying the gene encoding for the porcine rotavirus outer capsid protein VP7 in the E3 region of the Ad5 genome has been obtained. mRNA for VP7 in recombinant Ad5KV14/VP7 infected cells was demonstrated by means of RT-PCR. Radioimmunoprecipitation of cell extracts infected with Ad5KV14/VP7 on early (12 h p.i.) and late (24 h p.i.) stages of adenovirus infection with rabbit polyclonal antiserum raised to purified rotavirus virions revealed a recombinant protein possessing the same electrophoretic mobility, 37 kDa, as the native rotavirus K VP7. One-dimensional peptide mapping also demonstrated the identity of the recombinant and native VP7.
Doronin
K K
KK
Iurov
G K
GK
Grinenko
N F
NF
Zakharchuk
A N
AN
Denisova
T S
TS
Baburina
T M
TM
Krugliak
V A
VA
Naroditskiĭ
B S
BS
D000256:Adenoviridae / Q000235:genetics*; D000956:Antigens, Viral; D001483:Base Sequence; D002213:Capsid / Q000235:genetics*; D036022:Capsid Proteins; D017931:DNA Primers; D004591:Electrophoresis, Polyacrylamide Gel; D015967:Gene Expression Regulation, Viral; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D015531:Radioimmunoprecipitation Assay; D011995:Recombination, Genetic; D012401:Rotavirus / Q000235:genetics*
D000956:Antigens, Viral; D036022:Capsid Proteins; D017931:DNA Primers; C050779:VP7 protein, Rotavirus