A comparison between starch and polyacrylamide gels for the analysis of Listeria monocytogenes using multilocus enzyme electrophoresis.
Forty-seven Listeria monocytogenes isolates were analysed using multilocus enzyme electrophoresis in two laboratories. Both assayed for the same six enzymes, but one used a starch gel method and the other polyacrylamide gels. The starch gel method distinguished six electrophoretic types whereas the polyacrylamide gel method produced 17 different electrophoretic types. The polyacrylamide gel method was more discriminatory than the starch gel method.
Flint
S H
SH
New Zealand Dairy Research Institute, Palmerston North, New Zealand.
Hartley
N J
NJ
Avery
S M
SM
Hudson
J A
JA
D000263:Adenylate Kinase / Q000235:genetics / Q000302:isolation & purification; D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D004592:Electrophoresis, Starch Gel / Q000379:methods*; D004798:Enzymes / Q000235:genetics / Q000302:isolation & purification*; D004950:Esterases / Q000235:genetics / Q000302:isolation & purification; D005069:Evaluation Studies as Topic; D005516:Food Microbiology; D014644:Genetic Variation; D005956:Glucose-6-Phosphate Isomerase / Q000235:genetics / Q000302:isolation & purification; D005954:Glucosephosphate Dehydrogenase / Q000235:genetics / Q000302:isolation & purification; D005969:Glutamate Dehydrogenase / Q000235:genetics / Q000302:isolation & purification; D008089:Listeria monocytogenes / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D010430:Pentosyltransferases / Q000235:genetics / Q000302:isolation & purification
D004798:Enzymes; D005954:Glucosephosphate Dehydrogenase; D005969:Glutamate Dehydrogenase; D010430:Pentosyltransferases; C067995:nucleoside phosphorylase; D000263:Adenylate Kinase; D004950:Esterases; D005956:Glucose-6-Phosphate Isomerase