title: Prolactin and insulin regulate the release of IL-1-alpha and IFN-gamma from murine splenocytes activated with porins or LPS of Salmonella typhimurium.
abstract: Murine splenocytes treated with prolactin (PRL) or insulin were stimulated in vitro with porins or lipopolysaccharide (LPS) of Salmonella typhimurium. It was seen that PRL inhibits the release of IFN-gamma from splenocytes treated with porins by about 20% while having no effect on the release of IL-1-alpha. Splenocytes porin-stimulated splenocytes exhibited a remarkable increase in IL-1-alpha release (100%) and a diminished release of IFN-gamma (about 50%) in the presence of insulin. The splenocytes stimulated with LPS had a reduced release of IL-1-alpha (75%) and IFN-gamma (about 50%) when insulin was added. The data suggest that classical endocrine system participates in a bioregulatory feedback loop that may prevent unwanted toxicity from cytokine excess. However, some bacterial products sometimes enormously unbalance this regulatory network.
authors.affiliation: Istituto di Microbiologia, Facoltà di Medicina e Chirurgia, Seconda Università degli Studi di Napoli, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Vitiello
authors.forename: P
authors.initials: P
authors.lastname: Scarfogliero
authors.forename: M
authors.initials: M
authors.lastname: Galdiero
authors.forename: F
authors.initials: F
authors.lastname: Gorga
authors.forename: L
authors.initials: L
authors.lastname: Sommese
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D002478:Cells, Cultured; D006801:Humans; D007328:Insulin / Q000494:pharmacology*; D007371:Interferon-gamma / Q000378:metabolism*; D007375:Interleukin-1 / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000302:isolation & purification / Q000494:pharmacology; D008297:Male; D018272:Porins / Q000302:isolation & purification / Q000494:pharmacology; D011388:Prolactin / Q000494:pharmacology*; D012486:Salmonella typhimurium / Q000276:immunology*; D013154:Spleen / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; D007328:Insulin; D007375:Interleukin-1; D008070:Lipopolysaccharides; D018272:Porins; D007371:Interferon-gamma; D011388:Prolactin