Peroxides inhibit the glutathione S-conjugate pump.
Tert-Butyl hydroperoxide (100-300 microM) was found to inhibit the active efflux of dinitrophenyl-S-glutathione (DNP-SG) from human erythrocytes. From among amino acid peroxides generated by irradiation of amino acid (proline, valine and leucine) solutions, valine hydroperoxide (150 microM) had a similar effect on the DNP-SG transport. As the transport of glutathione S-conjugates is an important step of cellular detoxication, these results indicate that oxidative stress may impair cellular resistance to chemical stress of other kinds. t-Butyl and amino acid peroxides upon interaction with hemoglobin and whole erythrocytes produce free radicals which may be responsible for the damage to the glutathione S-conjugate pump of the erythrocyte membrane.
Soszyński
M
M
Department of Molecular Biophysics, University of Lódź, Poland.
Skalski
Z
Z
Pułaski
L
L
Bartosz
G
G
D001693:Biological Transport, Active / Q000187:drug effects; D002352:Carrier Proteins / Q000097:blood*; D004912:Erythrocytes / Q000187:drug effects*; D005609:Free Radicals; D005978:Glutathione / Q000031:analogs & derivatives* / Q000097:blood; D006801:Humans; D008658:Inactivation, Metabolic; D026901:Membrane Transport Proteins; D018384:Oxidative Stress / Q000187:drug effects*; D010545:Peroxides / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000494:pharmacology*; D020122:tert-Butylhydroperoxide
D002352:Carrier Proteins; D005609:Free Radicals; D026901:Membrane Transport Proteins; D010545:Peroxides; D017382:Reactive Oxygen Species; C086860:glutathione transporter; C030190:S-(2,4-dinitrophenyl)glutathione; D020122:tert-Butylhydroperoxide; D005978:Glutathione