Killing of arterial smooth muscle cells by chylomicron remnants.
Chylomicron remnants were found to be cytotoxic to cultured arterial smooth muscle cells. Cell death was estimated by two methods: colourimetric assay using a tetrazolium salt ahd trypan blue staining. Both methods showed considerable cell death. Twenty-five micrograms of cholesterol/ml of chylomicron remnants appeared not to injure smooth muscle cells; however, greater concentrations (= and >50 microg cholesterol/ml) caused considerable damage. Our observations may offer a possible cause of cell death in atherosclerotic plaques.
Yu
K C
KC
Department of Physiology, University of Western Australia, Perth, Australia.
Mamo
J C
JC
D000818:Animals; D001161:Arteriosclerosis / Q000209:etiology / Q000473:pathology; D016923:Cell Death / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002784:Cholesterol / Q000633:toxicity; D002914:Chylomicrons / Q000633:toxicity*; D004396:Coloring Agents; D009131:Muscle, Smooth, Vascular / Q000166:cytology* / Q000187:drug effects*; D011817:Rabbits; D013778:Tetrazolium Salts; D014343:Trypan Blue
D002914:Chylomicrons; D004396:Coloring Agents; D013778:Tetrazolium Salts; D002784:Cholesterol; D014343:Trypan Blue