Sporozoite transmission of three strains of Plasmodium knowlesi to Aotus and Saimiri monkeys.
Attempts were made to infect Aotus and Saimiri monkeys with sporozoites of 3 strains of Plasmodium knowlesi to determine the potential of these animals in a monkey/malaria model. Splenectomized Saimiri and Aotus monkeys were infected with the H strain of P. knowlesi via sporozoites from Anopheles dirus mosquitoes. Prepatent periods ranged from 5 to 16 days. Saimiri monkeys infected with the Philippine strain had prepatent periods ranging from 6 to 8 days. Saimiri monkeys infected with the Hackeri strain had prepatent periods ranging from 6 to 11 days. Exoerythrocytic (EE) stages of the Philippine strain were readily demonstrated; EE stages of the H strain were less abundant. Results indicate that the Philippine strain of P. knowlesi in Saimiri monkeys has a course of parasitemia and EE stages similar to those previously seen in macaques and could serve as a reproducible model for biologic and immunologic studies.
Sullivan
J S
JS
Division of Parasitic Diseases, National Center for Infectious Diseases, Atlanta, Georgia 30333, USA.
Morris
C L
CL
Richardson
B B
BB
Galland
G G
GG
Sullivan
J J
JJ
Collins
W E
WE
D000818:Animals; D000852:Anopheles; D001029:Aotus trivirgatus / Q000469:parasitology*; D004195:Disease Models, Animal; D004198:Disease Susceptibility; D006801:Humans; D007299:Insect Bites and Stings / Q000150:complications; D007303:Insect Vectors; D008288:Malaria / Q000276:immunology* / Q000635:transmission; D018512:Parasitemia / Q000276:immunology / Q000635:transmission; D016790:Plasmodium knowlesi / Q000276:immunology / Q000502:physiology*; D012453:Saimiri / Q000469:parasitology*