title: Effect of penicillin G on the electroporation of Rhodococcus rhodochrous CF222.
abstract: Suitable conditions for the introduction of bacteriophage DNA into cells of Rhodococcus rhodochrous CF222 by electroporation were established, and penicillin G was found to enhance the transfection frequency. When conditions optimal for the parental strain were applied to its colony-morphological mutants, different transfection frequencies were observed. Penicillin G enhanced the transfection frequency of smooth and mucoidal mutants but not of rough mutants.
authors.affiliation: Department of Applied Biological Science, College of Agriculture and Veterinary Medicine, Nihon University, Kanagawa, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Sunairi
authors.forename: N
authors.initials: N
authors.lastname: Iwabuchi
authors.forename: K
authors.initials: K
authors.lastname: Murakami
authors.forename: F
authors.initials: F
authors.lastname: Watanabe
authors.forename: Y
authors.initials: Y
authors.lastname: Ogawa
authors.forename: H
authors.initials: H
authors.lastname: Murooka
authors.forename: M
authors.initials: M
authors.lastname: Nakajima
mesh_terms: D001435:Bacteriophages / Q000235:genetics; D018274:Electroporation / Q000379:methods*; D005069:Evaluation Studies as Topic; D009154:Mutation; D010400:Penicillin G / Q000494:pharmacology*; D012240:Rhodococcus / Q000187:drug effects* / Q000235:genetics* / Q000254:growth & development; D014162:Transfection / Q000187:drug effects*
chemical_list: D010400:Penicillin G