title: Respiratory syncytial virus G-protein modulates cytokine release from human peripheral blood mononuclear cells.
abstract: Respiratory syncytial virus (RSV) is the major cause of lower respiratory tract infection in children and adults. In vivo the host has to cope with intact replicative virus, with non-replicative virus, and/or with viral structural proteins including the outer membrane G-protein. We analyzed the role of purified RSV G-protein with regard to its modulatory efficacy for interleukin (IL) -10, IL-12, and tumor necrosis factor-alpha (TNF-alpha) release from human peripheral blood mononuclear cells (PBMC). These cytokines seem to contribute to the deleterious effect in viral infections. Treatment of PBMC with RSV at a multiplicity of infection of 10 down to 0.001 induced the release of TNF-alpha, IL-10, and IL-12; also time kinetics and dose-responses differed markedly. Stimulation of PBMC with purified RSV G-protein (from 0.001 up to 10 microgram/10(6) PBMC) led only to a pronounced increase in IL-10 within a concentration range from 0.01 up to 0.5 microgram/10(6) PBMC with a maximum between 12 and 18 h of incubation. AT later time points (24, 48, and 72 h) G-protein concentrations above 1 microgram/10(6) PBMC suppressed IL-10, TNF-alpha, and IL-12 release from human PBMC. Coating of PBMC with RSV G-protein suppressed IL-10, TNF-alpha, and IL-12 release after subsequent stimulation with RSV. Our data indicate a regulatory role of RSV G-protein immune responses toward viral infection.
authors.affiliation: Department of Medical Microbiology and Immunology, AG Infektabwehr, Federal Republic of Germany.
authors.forename: B
authors.initials: B
authors.lastname: König
authors.forename: H J
authors.initials: HJ
authors.lastname: Streckert
authors.forename: T
authors.initials: T
authors.lastname: Krusat
authors.forename: W
authors.initials: W
authors.lastname: König
mesh_terms: D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism*; D004306:Dose-Response Relationship, Immunologic; D015707:HN Protein; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D018357:Respiratory Syncytial Virus Infections / Q000276:immunology*; D012136:Respiratory Syncytial Viruses / Q000276:immunology*; D013997:Time Factors; D014759:Viral Envelope Proteins; D014764:Viral Proteins / Q000276:immunology*
chemical_list: D016207:Cytokines; D015707:HN Protein; D014759:Viral Envelope Proteins; D014764:Viral Proteins; C116204:attachment protein G