Organisation of the mitochondrial genome of Trichophyton rubrum. DNA sequence analysis of the ND4 gene, the ATPase subunit-6 gene, the ribosomal RNA small-subunit gene, the ND6 gene, the COXIII gene, the ATPase subunit-8 gene and six tRNA genes that correspond respectively to the tyrosine, lysine, glutamine, asparagine, isoleucine and tryptophan isoacceptors.
We present the nucleotide sequence of a 5207-bp-long region of the mitochondrial genome of the dermatophyte Trichophyton rubrum. This represents about 1/5th of the total genome and extends a previous study. From the 5' end of the present sequence, the order of genes is as follows: the end of the ND4 gene, the gene coding for subunit 6 of ATPase, the gene coding for the small ribosomal RNA (SSU rRNA), the tyrosyl tRNA gene, the ND6 gene, the COXIII gene, the ATPase 8 subunit gene and a cluster of tRNAs genes corresponding respectively to the lysine, glutamine, asparagine, isoleucine and tryptophan isoacceptors. The interesting features of this region are its compact organisation, the presence of subunit 8 of the ATPase gene and the secondary structure of SSU rRNA which is close to that of Aspergillus nidulans. On the basis of the order of the genes, which is essentially similar to that of A. nidulans, we can also assume that the LSU rRNA subunit gene should be just upstream of this sequenced region.
de Bièvre
C
C
Unité de Mycologie, Institut Pasteur, Paris, France.
Dujon
B
B
D000251:Adenosine Triphosphatases / Q000235:genetics*; D000595:Amino Acid Sequence; D001233:Aspergillus nidulans / Q000235:genetics; D001483:Base Sequence; D004272:DNA, Mitochondrial / Q000737:chemistry / Q000235:genetics*; D015247:Deoxyribonuclease HindIII / Q000235:genetics; D008957:Models, Genetic; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009245:NADH Dehydrogenase / Q000235:genetics*; D009690:Nucleic Acid Conformation; D010940:Plant Proteins / Q000235:genetics; D012335:RNA, Ribosomal / Q000737:chemistry; D012343:RNA, Transfer; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014249:Trichophyton / Q000235:genetics*
D004272:DNA, Mitochondrial; C098483:NADH dehydrogenase subunit 4; D010940:Plant Proteins; D012335:RNA, Ribosomal; C075413:COXIII protein, Oryza sativa; D012343:RNA, Transfer; D009245:NADH Dehydrogenase; D015247:Deoxyribonuclease HindIII; D000251:Adenosine Triphosphatases