Antioxidation of human low density lipoprotein by unconjugated and conjugated bilirubins.
We demonstrate here that both unconjugated bilirubin (Bu) and conjugated bilirubin (Bc) can protect human low density lipoprotein(LDL) against oxidation by oxyradicals generated by 2,2'-azo-bis (2 amidinopropane) dihydrochloride at 37 degrees. The oxidation was assessed by agarose gel electrophoresis and was further corroborated by assaying the malondialdehydes and lipid peroxides formed throughout oxidation. On a per mole basis, Bu and less so Bc was more effective than ascorbate in preventing LDL oxidation. Since oxidative modification of human LDL was implicated in plaque formation in blood vessels leading to atherogenesis, the data suggested that either bile pigment may help reduce the risk of atherogenesis.
Wu
T W
TW
Department of Clinical Biochemistry and Surgery, University of Toronto, Ontario, Canada.
Fung
K P
KP
Wu
J
J
Yang
C C
CC
Weisel
R D
RD
D000578:Amidines / Q000378:metabolism / Q000494:pharmacology; D000975:Antioxidants / Q000494:pharmacology*; D001663:Bilirubin / Q000378:metabolism / Q000494:pharmacology*; D004586:Electrophoresis; D016166:Free Radical Scavengers / Q000494:pharmacology; D006801:Humans; D008054:Lipid Peroxides / Q000378:metabolism; D008077:Lipoproteins, LDL / Q000378:metabolism*; D008315:Malondialdehyde / Q000378:metabolism; D010545:Peroxides / Q000378:metabolism / Q000494:pharmacology
D000578:Amidines; D000975:Antioxidants; D016166:Free Radical Scavengers; D008054:Lipid Peroxides; D008077:Lipoproteins, LDL; D010545:Peroxides; C049375:perhydroxyl radical; D008315:Malondialdehyde; C046728:2,2'-azobis(2-amidinopropane); D001663:Bilirubin