Lophozozymus pictor toxin: a fluorescent structural isomer of palytoxin.
Purified Lophozozymus pictor toxin (LPTX) shares many properties similar to palytoxin (PTX). LPTX and palytoxin isolated from Palythoa caribaeorum (C-PTX) have similar mol. wts of approx. 2680 on ionspray mass spectrometry (MS). In addition, antibodies against PTX could recognize and bind LPTX. Mixed mode high-performance liquid chromatography (HPLC) of LPTX, C-PTX and H-PTX (isolated from Palythoa tuberculosa) showed a major PTX component common to all three with the characteristic PTX-like UV spectrum at a retention time (Rt) of 17 min. However, LPTX exhibits fluorescence but PTX of equivalent toxicity does not. LPTX showed a unique peak at Rt of approx. 22 min on mixed mode HPLC. In addition, LPTX and C-PTX showed different ion fragmentation patterns on MS/MS. These results suggest that LPTX and the palytoxins are structural isomers, containing at least one difference which gives rise to fluorescence in LPTX.
Lau
C O
CO
Department of Biochemistry, Faculty of Medicine, National University of Singapore.
Tan
C H
CH
Khoo
H E
HE
Yuen
R
R
Lewis
R J
RJ
Corpuz
G P
GP
Bignami
G S
GS
D000178:Acrylamides / Q000032:analysis / Q000737:chemistry*; D000911:Antibodies, Monoclonal; D002851:Chromatography, High Pressure Liquid; D003064:Cnidarian Venoms / Q000032:analysis / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay; D005453:Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism; D013729:Terpenes / Q000032:analysis / Q000737:chemistry*; D000095702:Polyether Toxins
D000178:Acrylamides; D000911:Antibodies, Monoclonal; D003064:Cnidarian Venoms; D013729:Terpenes; C030303:lophotoxin; C010272:palytoxin; D000095702:Polyether Toxins