Transport of the antibacterial agent (6S)-6-fluoroshikimate and other shikimate analogues by the shikimate transport system of Escherichia coli.
We show that the antibacterial agent, (6S)-6-fluoroshikimate, is a substrate for the shikimate transport system of Escherichia coli because in exchange-diffusion experiments it displaced intracellular [14C]shikimate with the same kinetics as did unlabelled shikimate. Other shikimate analogues were also substrates: as judged by similar experiments or, in the case of (6R)-6-fluoroshikimate, by inference.
Jude
D A
DA
ZENECA Pharmaceuticals, Department of Infection Research, Alderly Park, Macclesfield, UK.
Ewart
C D
CD
Thain
J L
JL
Davies
G M
GM
Nichols
W W
WW
D000900:Anti-Bacterial Agents / Q000378:metabolism*; D001692:Biological Transport; D002352:Carrier Proteins / Q000378:metabolism; D004058:Diffusion; D004926:Escherichia coli / Q000378:metabolism*; D007700:Kinetics; D012765:Shikimic Acid / Q000031:analogs & derivatives* / Q000378:metabolism*
C087313:6-fluoroshikimic acid; D000900:Anti-Bacterial Agents; D002352:Carrier Proteins; D012765:Shikimic Acid