title: Effect of essential fatty acid deficiency on G-proteins, cAMP-dependent protein kinase activity and mucin secretion in the rat submandibular salivary glands.
abstract: Studies were conducted to determine whether beta-adrenergic cell signalling is altered in submandibular salivary glands (SMSG) is essential fatty acid (EFA) deficiency. Three groups of rats were fed diets which were deficient in EFA (EFAD), marginally deficient in EFA (MEFAD) or contained sufficient amount of EFA (Control). Rats were killed after 20 wk on diets, SMSG were dissected out and cyclic AMP-dependent protein kinase (PKA) activity was measured. The specific enzyme activities were higher in the homogenates and supernatant fractions of the gland from EFAD and MEFAD rats compared with the controls. The relative levels of guanine nucleotide-binding regulatory proteins (Gs and Gi) were also measured in the SMSG membranes of rats fed the 3 diets. The levels of Gs were significantly higher in the EFAD and MEFAD groups than in the controls. No significant differences were observed in the secretion of trichloroacetic acid-phosphotungstic acid (TCA-PTA) precipitable glycoproteins from the SMSG slices among the 3 dietary groups.
authors.affiliation: Department of Biochemistry and Molecular Biology, Louisiana State University Medical Center, New Orleans 70119, USA.
authors.forename: S Q
authors.initials: SQ
authors.lastname: Alam
authors.forename: S M
authors.initials: SM
authors.lastname: Abdel-Hakim
authors.forename: B S
authors.initials: BS
authors.lastname: Alam
authors.forename: I Y
authors.initials: IY
authors.lastname: Ibrahim
mesh_terms: D000262:Adenylyl Cyclases / Q000378:metabolism; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D000242:Cyclic AMP / Q000502:physiology; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism*; D004041:Dietary Fats / Q000008:administration & dosage; D005228:Fatty Acids, Essential / Q000172:deficiency* / Q000378:metabolism; D019204:GTP-Binding Proteins / Q000378:metabolism*; D008297:Male; D009077:Mucins / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011943:Receptors, Adrenergic, beta / Q000502:physiology*; D012463:Saliva / Q000378:metabolism; D012471:Salivary Proteins and Peptides / Q000378:metabolism*; D012636:Secretory Rate / Q000187:drug effects; D015398:Signal Transduction; D013363:Submandibular Gland / Q000378:metabolism*
chemical_list: D004041:Dietary Fats; D005228:Fatty Acids, Essential; D009077:Mucins; D011943:Receptors, Adrenergic, beta; D012471:Salivary Proteins and Peptides; D000242:Cyclic AMP; D017868:Cyclic AMP-Dependent Protein Kinases; D019204:GTP-Binding Proteins; D000262:Adenylyl Cyclases