Suspension culture of HeLa cells in protein-free medium: sensitivity to human pathogenic viruses.
Human adherent HeLa-PF cells grown for 5 years in a protein-free 1:1 nutrient mixture of Dulbecco's modified MEM and Ham's F12 (DMEM-F12) were established in suspension culture. The cells grew in protein-free DMEM-F12 (using magnetically stirred flasks) as a monodisperse suspension with a population doubling time of 28 h. The cells were infected with poliovirus types 2 and 3, herpes simplex virus types 1 and 2 (HSV-1 and HSV-2), respiratory syncytial virus (RSV), echovirus 6 and adenoviruses 3 and 7. Polioviruses replicated in suspension culture of HeLa-PF cells to a similar extent as in protein-free and serum-supplemented adherent cultures. RSV and HSV developed significantly lower titers while adenoviruses and echovirus 6 developed significantly higher titers in suspension than in adherent cultures. The results show that suspension culture of HeLa-PF may both provide the advantage of a high virus yield and enable cultivation of viruses which are not contaminated by serum or other proteins usually added to the culture medium.
Cinatl
J
J
Centre of Hygiene, J.W. Goethe University, Frankfurt, Germany.
Cinatl
J
J
Wichelhaus
T
T
Weber
B
B
Rabenau
H
H
Gümbel
H O
HO
Kornhuber
B
B
Doerr
H W
HW
D018929:Cell Culture Techniques / Q000379:methods; D002455:Cell Division; D003470:Culture Media; D006367:HeLa Cells; D006801:Humans; D014776:Virus Cultivation / Q000379:methods*; D014780:Viruses / Q000254:growth & development*
D003470:Culture Media