Tissue-specific expression of an anti-ras ribozyme inhibits proliferation of human malignant melanoma cells.
In this study, we have compared the efficacy of a tissue-specific promoter (tyrosinase promoter) with a viral promoter to express anti-ras ribozyme RNA in human melanoma cells. The retroviral vector containing the tyrosinase promoter was superior in its ability to suppress the human melanoma phenotype in vitro as characterized by changes in growth, melanin synthesis, morphology and H-ras gene expression. These data support the use of tissue-specific expression of anti-oncogene ribozymes as a rational therapeutic strategy in human cancers.
Ohta
Y
Y
Department of Medical Oncology, City of Hope National Medical Center, Duarte, CA 91010, USA.
Kijima
H
H
Ohkawa
T
T
Kashani-Sabet
M
M
Scanlon
K J
KJ
D001483:Base Sequence; D002455:Cell Division / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D018014:Gene Transfer Techniques; D011905:Genes, ras; D006801:Humans; D008545:Melanoma / Q000235:genetics* / Q000473:pathology; D008969:Molecular Sequence Data; D016337:RNA, Catalytic / Q000235:genetics*; D014407:Tumor Cells, Cultured
D016337:RNA, Catalytic