Cloning and characterization of a TEF gene for elongation factor 1 alpha from the yeast Arxula adeninivorans.
The translation elongation factor EF-1 alpha appears to play a major role in the control of cell proliferation and ageing in higher eukaryotes. Here we report the cloning of the TEF1 gene encoding the elongation factor 1 alpha of the dimorphic yeast Arxula adeninivorans Ls3. The gene is localized on chromosome 2 from Arxula adeninivorans, comprises 1380 bp and encodes a protein containing 459 amino acids. In contrast to other fungi, a second TEF gene encoding an identical, or nearly identical, polypeptide could not be identified. The transcriptional activity of the TEF1 gene did not change during mycelial growth, whereas a slight decrease could be detected during the yeast growth.
Rösel
H
H
Institute of Plant Genetics and Crop Plant Research, Gatersleben, Germany.
Kunze
G
G
D000595:Amino Acid Sequence; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D015152:Blotting, Northern; D015139:Blotting, Southern; D002455:Cell Division; D015825:Chromosomes, Fungal / Q000235:genetics; D003001:Cloning, Molecular; D016521:Electrophoresis, Gel, Pulsed-Field; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics*; D018628:Gene Dosage; D005800:Genes, Fungal / Q000235:genetics*; D008969:Molecular Sequence Data; D020648:Peptide Elongation Factor 1; D010445:Peptide Elongation Factors / Q000737:chemistry / Q000235:genetics*; D014176:Protein Biosynthesis / Q000235:genetics; D015183:Restriction Mapping; D016385:TATA Box / Q000235:genetics; D014158:Transcription, Genetic / Q000235:genetics; D015003:Yeasts / Q000737:chemistry / Q000235:genetics*
D005656:Fungal Proteins; D020648:Peptide Elongation Factor 1; D010445:Peptide Elongation Factors