Temporal differences in praziquantel- and oxamniquine-induced tegumental damage to adult Schistosoma mansoni: implications for drug-antibody synergy.
A temporal study of the effects on the tegument of Schistosoma mansoni adult worm following in vivo praziquantel and oxamniquine treatment was performed. Drug-induced damage to the tegument, exposure of surface antigens and attachment of host antibody occurred rapidly, within 1 h, following praziquantel treatment. Oxamniquine-treated worms required 4-8 days for these effects to be apparent. The 2 drugs differed in the degree and sites of damage on the worm surface. The administration of 2 different polyspecific rabbit sera with drug significantly increased the efficacy of praziquantel when administered with the drug, but not when given 6-9 days after drug treatment. In contrast, only 1 serum was synergistic with oxamniquine when administered with drug and both sera were synergistic when given 6-9 days after drug treatment. The effect of immune killing of drug-treated worms is discussed.
Fallon
P G
PG
School of Biological Sciences, University of Wales, Bangor, Gwynedd, UK.
Fookes
R E
RE
Wharton
G A
GA
D000818:Animals; D000909:Antibodies, Helminth / Q000008:administration & dosage / Q000276:immunology*; D000947:Antigens, Helminth / Q000276:immunology; D015153:Blotting, Western; D004357:Drug Synergism; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007116:Immunization, Passive; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D008855:Microscopy, Electron, Scanning; D010073:Oxamniquine / Q000494:pharmacology*; D011223:Praziquantel / Q000494:pharmacology*; D011817:Rabbits; D012550:Schistosoma mansoni / Q000187:drug effects* / Q000276:immunology / Q000648:ultrastructure; D012555:Schistosomiasis mansoni / Q000188:drug therapy*; D012556:Schistosomicides / Q000494:pharmacology*; D012908:Snails
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D012556:Schistosomicides; D010073:Oxamniquine; D011223:Praziquantel