title: Preparation of recombinant Rab geranylgeranyltransferase and Rab escort proteins.
authors.affiliation: Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas 75235, USA.
authors.forename: S A
authors.initials: SA
authors.lastname: Armstrong
authors.forename: M S
authors.initials: MS
authors.lastname: Brown
authors.forename: J L
authors.initials: JL
authors.lastname: Goldstein
authors.forename: M C
authors.initials: MC
authors.lastname: Seabra
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D019883:Alkyl and Aryl Transferases; D000595:Amino Acid Sequence; D000818:Animals; D016367:Baculoviridae; D002352:Carrier Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification*; D002460:Cell Line; D002850:Chromatography, Gel / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000379:methods; D003001:Cloning, Molecular / Q000379:methods; D003600:Cytosol / Q000201:enzymology; D018076:DNA, Complementary; D006639:Histidine; D006801:Humans; D007202:Indicators and Reagents; D007700:Kinetics; D008969:Molecular Sequence Data; D011106:Polyisoprenyl Phosphates / Q000378:metabolism; D017368:Protein Prenylation; D051381:Rats; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000302:isolation & purification; D016324:Sequence Tagged Sites; D018411:Spodoptera; D013379:Substrate Specificity; D014162:Transfection / Q000379:methods; D014166:Transferases / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification*; D020691:rab GTP-Binding Proteins
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C081787:CHM protein, human; D002352:Carrier Proteins; C513166:Chm protein, rat; D018076:DNA, Complementary; D007202:Indicators and Reagents; D011106:Polyisoprenyl Phosphates; D011994:Recombinant Proteins; D006639:Histidine; D014166:Transferases; D019883:Alkyl and Aryl Transferases; C075607:Rab geranylgeranyltransferase; D020691:rab GTP-Binding Proteins; C002963:geranylgeranyl pyrophosphate