Defects in hemostasis in P-selectin-deficient mice.
Recently, our laboratory showed that platelets, like leukocytes, roll on activated endothelium expressing P-selectin, thus suggesting a role for P-selectin in hemostasis (Frenette et at, Proc Natl Acad Sci USA 92:7450, 1995). We report here that the P-selectin--deficient mice show a 40% prolongation of the bleeding time on amputation of the tip of the tail. Moreover, defective hemostasis was observed in a local Shwartzman-like reaction induced by skin injections of lipopolysaccharide followed by tumor necrosis factor-alpha in the P-selectin--deficient mice. The hemorrhagic lesions, quantitated both macroscopically and microscopically, were twofold larger in the P-selectin--deficient mice. This was also confirmed by measuring the radioactivity in the skin using chromium-labeled red blood cells. Therefore, it is evident that P-selectin plays a role in hemostasis as suggested by its support of platelet rolling.
Subramaniam
M
M
Center for Blood Research, Boston, MA 02115, USA.
Frenette
P S
PS
Saffaripour
S
S
Johnson
R C
RC
Hynes
R O
RO
Wagner
D D
DD
D000818:Animals; D001760:Bleeding Time; D006470:Hemorrhage / Q000503:physiopathology; D006487:Hemostasis; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D019007:P-Selectin / Q000502:physiology*; D012790:Shwartzman Phenomenon
D019007:P-Selectin