The counterion influence on cationic lipid-mediated transfection of plasmid DNA.
A panel of DOTAP analogs was prepared by altering the anionic counterion that accompanies the trimethylammonium polar domain. The transfection of plasmid DNA into NIH3T3 cells and mouse lung was examined using the counterion analogs. The in vitro transfection activity decreased as follows: DOTAP.bisulfate > trifluoromethanesulfonate approximately equal to iodide approximately equal to bromide > dihydrogenphosphate approximately equal to chloride approximately equal to acetate > sulfate. A similar activity trend was observed in vivo.
Aberle
A M
AM
Department of Chemistry, University of California, Davis, 95616, USA.
Bennett
M J
MJ
Malone
R W
RW
Nantz
M H
MH
D016475:3T3 Cells; D000818:Animals; D005229:Fatty Acids, Monounsaturated / Q000737:chemistry*; D007322:Instillation, Drug; D007477:Ions; D008081:Liposomes; D008168:Lung; D051379:Mice; D008807:Mice, Inbred BALB C; D010957:Plasmids / Q000235:genetics*; D000644:Quaternary Ammonium Compounds / Q000737:chemistry*; D014132:Trachea; D014162:Transfection / Q000379:methods*
D005229:Fatty Acids, Monounsaturated; D007477:Ions; D008081:Liposomes; D000644:Quaternary Ammonium Compounds; C070046:1,2-dioleoyloxy-3-(trimethylammonium)propane