Structure of the O-specific polysaccharide chain of Shigella boydii type 5 lipopolysaccharide: a repeated study.
An acidic, partially O-acetylated O-specific polysaccharide was obtained by mild acid degradation of Shigella boydii type 5 lipopolysaccharide and studied by 1H and 13C NMR spectroscopy, including 2D COSY, 13C-1H heteronuclear COSY, 1D NOE, and 2D ROESY experiments, and chemical methods (sugar and methylation analysis, O-deacetylation, carboxyl reduction, solvolysis with anhydrous HF, partial acid hydrolysis. Smith degradation). It was concluded that the polysaccharide has a hexasaccharide repeating unit of the following structure: [Formula: See Text] with the degree of O-acetylation varying over 30-50%. The established structure differs from that proposed recently for the O-specific polysaccharide of the same S. boydii serotype [M.J. Albert et al., Carbohydr. Res., 265 (1994) 121-127].
L'vov
V L
VL
Institute of Immunology, Moscow, Russian Federation.
Shashkov
A S
AS
Knirel
Y A
YA
Arifulina
A E
AE
Senchenkova
S N
SN
Yakovlev
A V
AV
Dmitriev
B A
BA
D000107:Acetylation; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D019081:O Antigens / Q000737:chemistry*; D012761:Shigella boydii / Q000737:chemistry*
D019081:O Antigens