title: Mild posttraumatic hypothermia reduces mortality after severe controlled cortical impact in rats.
abstract: The effect of posttraumatic hypothermia (brain temperature controlled at 32 degrees C for 4 h) on mortality after severe controlled cortical impact (CCI) was studied in rats. Four posttraumatic brain temperatures were compared: 37 degrees C (n = 10), 36 degrees C (n = 4), 32 degrees C (n = 10), and uncontrolled (UC; n = 6). Rats were anesthetized and subjected to severe CCI (4.0-m/s velocity, 3.0-mm depth) to the exposed left parietal cortex. At 10 min posttrauma the rats were cooled or maintained at their target brain temperature, using external cooling or warming. Brain temperature in the UC group was recorded but not regulated, and rectal temperature was maintained at 37 +/- 0.5 degrees C. After 4 h, rats were rewarmed over a 1-h period to 37 degrees C, extubated, and observed for 24 h. In the 37 and 36 degree C groups, 24-h mortality was 50% (37 degrees C = 5/10, 36 degrees C = 2/4). In the 32 degree C group, 24-h mortality was 10% (1/10). In the UC group, brain temperature was 35.4 +/- 0.6 degrees C during the 4-h treatment period and 24-h mortality was 0% (0/6). Mortality was higher in groups with brain temperatures > or = 36 degrees C versus those with brain temperatures < 36 degrees C (50 vs. 6%, respectively; p < 0.05). Additionally, electroencephalograms (EEG) were recorded in subsets of each temperature group and the percentage of time that the EEG was suppressed (isoelectric) was determined. Percentage of EEG suppression was greater in the hypothermic (32 degrees C, n = 6; UC, n = 4) groups than in the normothermic (36 degrees C, n = 3; 37 degrees C, n = 6) groups (23.3 +/- 14.3 vs. 1.2 +/- 3.1%, respectively; p < 0.05). Posttraumatic hypothermia suppressed EEG during treatment and reduced mortality after severe CCI. The threshold for this protective effect appears to be a brain temperature < 36 degrees C. Thus, even mild hypothermia may be beneficial after severe brain trauma.
authors.affiliation: Department of Anesthesiology, University of Pittsburgh, PA, USA.
authors.forename: R S
authors.initials: RS
authors.lastname: Clark
authors.forename: P M
authors.initials: PM
authors.lastname: Kochanek
authors.forename: D W
authors.initials: DW
authors.lastname: Marion
authors.forename: J K
authors.initials: JK
authors.lastname: Schiding
authors.forename: M
authors.initials: M
authors.lastname: White
authors.forename: A M
authors.initials: AM
authors.lastname: Palmer
authors.forename: S T
authors.initials: ST
authors.lastname: DeKosky
mesh_terms: D000818:Animals; D001831:Body Temperature; D001930:Brain Injuries / Q000503:physiopathology / Q000628:therapy*; D004569:Electroencephalography; D007036:Hypothermia, Induced; D008297:Male; D010296:Parietal Lobe / Q000293:injuries*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012640:Seizures / Q000209:etiology / Q000503:physiopathology