Characterization of DBT cell clones derived from cells persistently infected with the JHM strain of mouse hepatitis virus.
Twelve clones derived from the cells persistently infected with the JHM strain (JHMV) of mouse hepatitis virus (MHV) were established from mouse astrocytoma-derived DBT cells and characterized. All the cell clones were resistant to superinfection with MHV. Only one of the persistently infected cell clone synthesized viral RNA and proteins and produced virus particles. Viral RNA was detectable in some other cell clones without production of viral protein nor the virus. No cell clones exhibited contact fusion activity. The results suggested that such variety of cell clones might have resulted from persistent infection with JHMV.
Maeda
A
A
Department of Laboratory Animal Science, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
Hayashi
M
M
Ishida
K
K
Mizutani
T
T
Watanabe
T
T
Namioka
S
S
D000818:Animals; D001254:Astrocytoma / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D001483:Base Sequence; D001923:Brain Chemistry; D001932:Brain Neoplasms / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D002999:Clone Cells; D018352:Coronavirus Infections / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D017931:DNA Primers / Q000032:analysis / Q000737:chemistry / Q000235:genetics; D004279:DNA, Viral / Q000032:analysis / Q000737:chemistry / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D006517:Murine hepatitis virus / Q000235:genetics / Q000502:physiology*; D009154:Mutation; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012367:RNA, Viral / Q000032:analysis / Q000737:chemistry / Q000235:genetics; D012376:Rodent Diseases / Q000235:genetics / Q000473:pathology* / Q000821:virology; D014407:Tumor Cells, Cultured; D010948:Viral Plaque Assay / Q000662:veterinary
D017931:DNA Primers; D004279:DNA, Viral; D012367:RNA, Viral