Establishment and characterization of a new human mesothelioma cell line (T-85) from malignant peritoneal mesothelioma with remarkable thrombocytosis.
A mesothelioma cell line, termed T-85, was established from a patient with malignant peritoneal mesothelioma and remarkable thrombocytosis (1.4 x 10(6)/mm3). Electron microscopically, two types of mesothelioma cells have been characterized; the major type of cells with dense-cored granules in the cytoplasm and the minor one with evenly dense granules. Immunologically, the cells showed staining for interleukin-6 (IL-6), cytokeratin, collagen type IV, vimentin, laminin, fibronectin and Factor VIII-related antigen. Quantitation by ELISA revealed a high concentration of IL-6 in T-85 cell culture supernatants. RT-polymerase chain reaction of T-85 cells showed two positive bands of cDNA at 628 and 251 base pairs indicating the constitutive expression of IL-6 and IL-6 receptor mRNA. Moreover, prominent pro-platelet process formation activity in T-85 cell culture supernatants indicated the presence of a thrombopoietic activity due mainly to IL-6 but not the c-Mpl ligand or erythropoietin. However, the fact that 15% of PPF activity remained in the supernatants treated with anti-IL-6 antibody indicated the presence of another thrombopoietic substance. T-85 is so far the first mesothelioma cell line derived from a case with remarkable thrombocytosis.
Tange
T
T
Department of Pathology, Faculty of Medicine, University of Tokyo, Japan.
Hasegawa
Y
Y
Oka
T
T
Sunaga
S
S
Higashihara
M
M
Matsuo
K
K
Miyazaki
H
H
Shimosaka
A
A
Okano
A
A
Todokoro
K
K
D001483:Base Sequence; D001792:Blood Platelets / Q000166:cytology; D002454:Cell Differentiation; D002474:Cell-Free System / Q000737:chemistry; D004921:Erythropoietin / Q000032:analysis; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D008533:Megakaryocytes / Q000166:cytology; D008654:Mesothelioma / Q000737:chemistry / Q000473:pathology* / Q000648:ultrastructure; D008875:Middle Aged; D008969:Molecular Sequence Data; D013922:Thrombocytosis / Q000378:metabolism / Q000473:pathology*; D013926:Thrombopoietin / Q000032:analysis; D014407:Tumor Cells, Cultured
D004921:Erythropoietin; D013926:Thrombopoietin