Disordered expression of hepatic glycolytic and gluconeogenic enzymes in Otsuka Long-Evans Tokushima fatty rats with spontanteous long-term hyperglycemia.
Expression of key regulatory enzymes involved in glucose metabolism was studied in the livers of Otsuka Long-Evans Tokushima fatty (OLETF) rats, a model of non-insulin dependent diabetes mellitus. The activity and mRNA levels of glucokinase and L-type pyruvate kinase was increased in the liver of OLETF rats compared with control rats. There was no such remarkable change in liver-type phosphofructokinase. The activities of glucose-6-phosphatase and fructose-1,6-biphosphatase also increase despite high plasma levels of glucose and insulin. The activity of phosphoenolpyruvate carboxykinase did not show any significant change. The mRNA levels for fructose-1,6-biphosphatase, and phosphoenolpyruvate carboxykinase exhibited no marked changes. These results suggest that the expression of glucose-6-phosphatase and fructose-1,6-biphosphatase is disordered in OLETF rats.
Hotta
K
K
Second Department of Internal Medicine, Osaka University Medical School, Japan.
Kuwajima
M
M
Ono
A
A
Nakajima
H
H
Shingu
R
R
Miyagawa
J
J
Namba
M
M
Hanafusa
T
T
Noguchi
T
T
Kono
N
N
Matsuzawa
Y
Y
D000818:Animals; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic; D005941:Glucokinase / Q000235:genetics; D005943:Gluconeogenesis; D006019:Glycolysis; D006943:Hyperglycemia / Q000378:metabolism*; D008099:Liver / Q000201:enzymology*; D008297:Male; D010732:Phosphofructokinase-1 / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats
D012333:RNA, Messenger; D010732:Phosphofructokinase-1; D005941:Glucokinase