Rapid, simple serodiagnosis of murine typhus.
The dot-blot enzyme-linked immunosorbent assay (dot-ELISA) was compared to latex agglutination (LA) and the Weil-Felix OX-19 test for the diagnosis of murine typhus using the indirect immunofluorescent antibody (IFA) test as the 'gold standard'. With a panel of 74 positive and 47 negative sera, the dot-ELISA was 98% specific and 74% sensitive at a cut-off value of the second dot. With acute sera, latex agglutination was 100% specific and 74% sensitive at a cut-off titre of > or = 1:64. Both tests were more sensitive than the OX-19 test, which was 98% specific and 56% sensitive at a titre of > or = 1:320. Both dot-ELISA and latex agglutination were comparable and the results were available within one hour of testing. The rapidity, ease in performance and minimal requirement for electrical instruments made these 2 tests suitable for the diagnosis of murine typhus in countries where sophisticated laboratory facilities are lacking.
Silpapojakul
K
K
Department of Medicine, Faculty of Medicine, Prince of Songkla University, Thailand.
Pradutkanchana
J
J
Pradutkanchana
S
S
Kelly
D J
DJ
D000208:Acute Disease; D000372:Agglutination Tests; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005455:Fluorescent Antibody Technique; D006801:Humans; D007841:Latex Fixation Tests; D012680:Sensitivity and Specificity; D013997:Time Factors; D014437:Typhus, Endemic Flea-Borne / Q000175:diagnosis*