Developmental expression of calmodulin mRNA and protein in regions of the postnatal rat brain.
The expression of calmodulin (CaM) protein and mRNA was analyzed in specific regions of the rat brain during postnatal development. CaM levels in the adult brain were more abundant in the cerebral hemispheres and thalamus compared to brain stem and superior plus inferior colliculus. All brain regions contained higher CaM protein and mRNA levels than in non-neural tissues such as the kidney. During postnatal development of the brain, maximal levels of CaM protein and CaM I mRNAs were attained at day 10 or 15. Protein levels declined thereafter in the adult in all regions except the thalamus. With respect to products of the rat CaM I gene, the 4.0 kb neural transcript demonstrated a pronounced increase during postnatal development, whereas the 1.8 kb message showed little change.
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Department of Zoology, University of Toronto, Ontario, Canada.
Brown
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IR
D000818:Animals; D000831:Animals, Newborn; D015152:Blotting, Northern; D015153:Blotting, Western; D001921:Brain / Q000254:growth & development / Q000378:metabolism*; D002147:Calmodulin / Q000096:biosynthesis* / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D018507:Gene Expression Regulation, Developmental; D015347:RNA Probes; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar
D002147:Calmodulin; D015347:RNA Probes; D012333:RNA, Messenger