A mammalian histone deacetylase related to the yeast transcriptional regulator Rpd3p.
Trapoxin is a microbially derived cyclotetrapeptide that inhibits histone deacetylation in vivo and causes mammalian cells to arrest in the cell cycle. A trapoxin affinity matrix was used to isolate two nuclear proteins that copurified with histone deacetylase activity. Both proteins were identified by peptide microsequencing, and a complementary DNA encoding the histone deacetylase catalytic subunit (HD1) was cloned from a human Jurkat T cell library. As the predicted protein is very similar to the yeast transcriptional regulator Rpd3p, these results support a role for histone deacetylase as a key regulator of eukaryotic transcription.
Taunton
J
J
Howard Hughes Medical Institute, Harvard University, Cambridge, MA 02138, USA.
Hassig
C A
CA
Schreiber
S L
SL
D000595:Amino Acid Sequence; D000818:Animals; D000900:Anti-Bacterial Agents / Q000378:metabolism / Q000494:pharmacology; D002417:Cattle; D002453:Cell Cycle / Q000187:drug effects; D003001:Cloning, Molecular; D004791:Enzyme Inhibitors / Q000378:metabolism / Q000494:pharmacology; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation; D056572:Histone Deacetylase Inhibitors; D006655:Histone Deacetylases / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D006801:Humans; D006877:Hydroxamic Acids / Q000378:metabolism / Q000494:pharmacology; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010455:Peptides; D012441:Saccharomyces cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D013601:T-Lymphocytes / Q000201:enzymology; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
D000900:Anti-Bacterial Agents; D004791:Enzyme Inhibitors; D005656:Fungal Proteins; D056572:Histone Deacetylase Inhibitors; D006877:Hydroxamic Acids; D010455:Peptides; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; C067070:trapoxin A; C012589:trichostatin A; C071665:RPD3 protein, S cerevisiae; D006655:Histone Deacetylases