title: Amyotrophic lateral sclerosis cerebrospinal fluid is not toxic to cultured spinal motor neurons.
abstract: We have studied an effect of cerebrospinal fluid (CSF) from patients with amyotrophic lateral sclerosis (ALS) on explanted and dissociated ventral spinal cord cultures from 13-day-old rat embryos. CSF samples were obtained from 10 ALS patients, 10 other neurological patients, and 10 non-neurological patients. CSF was added at dilution of 10, 25, and 50%. Neurite length and neuronal survival ratio were not significantly different among these three groups. ALS CSF does not contain a neurotoxic factor on the cultured spinal motor neurons.
authors.affiliation: Fourth Department of Internal Medicine, Toho University Ohashi Hospital, Tokyo, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Iwasaki
authors.forename: K
authors.initials: K
authors.lastname: Ikeda
authors.forename: T
authors.initials: T
authors.lastname: Shiojima
authors.forename: M
authors.initials: M
authors.lastname: Tagaya
authors.forename: M
authors.initials: M
authors.lastname: Kinoshita
mesh_terms: D000328:Adult; D000368:Aged; D000690:Amyotrophic Lateral Sclerosis / Q000134:cerebrospinal fluid*; D000818:Animals; D002470:Cell Survival; D002478:Cells, Cultured / Q000166:cytology; D004622:Embryo, Mammalian / Q000166:cytology; D006801:Humans; D007201:Indicator Dilution Techniques; D008875:Middle Aged; D009046:Motor Neurons / Q000166:cytology* / Q000648:ultrastructure; D016501:Neurites / Q000502:physiology; D009498:Neurotoxins / Q000134:cerebrospinal fluid / Q000494:pharmacology; D009849:Olivopontocerebellar Atrophies / Q000134:cerebrospinal fluid; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000166:cytology
chemical_list: D009498:Neurotoxins