A CUC triplet confers leucine-dependent regulation of the Bacillus subtilis ilv-leu operon.
Regulation of the ilv-leu operon probably involves interaction of a tR NA(GAG) with leader region mRNA. Conversion of a CUC (Leu) triplet located within the leader region to UUC (Phe), CGC (Arg), or UAC (Tyr) converted reporter gene expression to control by corresponding amino acids. Conversion of the CUC triplet to CUU (Leu) decreased expression and disrupted regulation. The results suggested that other tRNAs can substitute for tRNA(Leu) but that interactions in addition to pairing of the anticodon with the CUC triplet are important for proper control.
Marta
P T
PT
Department of Molecular Biology, University of Medicine and Dentistry of New Jersey School of Osteopathic Medicine, Stratford, New Jersey 08084, USA.
Ladner
R D
RD
Grandoni
J A
JA
D001412:Bacillus subtilis / Q000235:genetics*; D001483:Base Sequence; D001665:Binding Sites; D003062:Codon; D015964:Gene Expression Regulation, Bacterial; D007763:Lac Operon; D007930:Leucine / Q000235:genetics*; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009690:Nucleic Acid Conformation; D016366:Open Reading Frames; D009876:Operon; D012329:RNA, Bacterial / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D013329:Structure-Activity Relationship; D014158:Transcription, Genetic
D003062:Codon; D012329:RNA, Bacterial; D012333:RNA, Messenger; D007930:Leucine