title: Analysis of rye B-chromosome structure using fluorescence in situ hybridization (FISH).
abstract: Fluorescence in situ hybridization (FISH) has been used to analyse the structure of the rye B chromosome. Genomic in situ hybridization (GISH) demonstrates the high level of overall similarity between A and B chromosomes of rye, as well as the presence of a number of specific sequences. The B-specific repeat families D1100 and E3900 have been analysed in terms of their physical location and possible contiguity. Rye Bs contain members of the rye-specific dispersed repetitive family R173, as well as centromeric regions similar to those of the As. The B chromosomes analysed in our study lack detectable rDNA sequences. Anomalous results have been obtained with a number of subtelomeric repetitive probes from rye. Bs usually lack these sequences, but evidence is presented that in some cases A-B translocation events may relocate such sequences from the As to the Bs. These data are discussed in the context of current models for the origin of the B chromosome.
authors.affiliation: Institute of Biological Sciences, University of Wales, Aberystwyth, UK.
authors.forename: T M
authors.initials: TM
authors.lastname: Wilkes
authors.forename: M G
authors.initials: MG
authors.lastname: Francki
authors.forename: P
authors.initials: P
authors.lastname: Langridge
authors.forename: A
authors.initials: A
authors.lastname: Karp
authors.forename: R N
authors.initials: RN
authors.lastname: Jones
authors.forename: J W
authors.initials: JW
authors.lastname: Forster
mesh_terms: D002875:Chromosomes / Q000648:ultrastructure*; D018744:DNA, Plant / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D006570:Heterochromatin / Q000648:ultrastructure; D017404:In Situ Hybridization, Fluorescence; D012091:Repetitive Sequences, Nucleic Acid; D012434:Secale / Q000235:genetics* / Q000648:ultrastructure
chemical_list: D018744:DNA, Plant; D004275:DNA, Ribosomal; D006570:Heterochromatin