title: Effects of UVC irradiation on cultured mouse embryonic limb bud cells.
abstract: Effects of UVC irradiation (UVC) at a dose range from 0 to 30 J/m(2) were investigated on the cultured embryonic limb bud cells (LBC), isolated from fore- and hindlimbs of day 11 mouse embryos. Although dose-dependent inhibition was found for both cellular proliferation and chondrogenesis, the chondrogenic proteoglycan (PG) synthesis was more sensitive to UVC than cellular proliferation when compared at ID(50), the inhibitory dose that reduced assessment value by 50% of the control. No significant difference in induction and repair kinetics of UV-induced cyclobutane pyrimidine dimers (CPDs) and (6-4) photoproducts ((6-4)PPs) were found between LBC and NIH3T3 mouse cell line. The fluorescent light (FL) treatment of LBC pre or post UVC irradiation did not affect repair kinetics of CPDs and (6-4)PPs, cellular proliferation, formation of PG-producing nodule and the PG synthesis.
authors.affiliation: Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Japan.
authors.forename: B
authors.initials: B
authors.lastname: Wang
authors.forename: K
authors.initials: K
authors.lastname: Fujita
authors.forename: N
authors.initials: N
authors.lastname: Uchida
authors.forename: H
authors.initials: H
authors.lastname: Mitani
authors.forename: T
authors.initials: T
authors.lastname: Yamada
authors.forename: A
authors.initials: A
authors.lastname: Shima
mesh_terms: D016475:3T3 Cells / Q000528:radiation effects; D000818:Animals; D002356:Cartilage / Q000196:embryology / Q000528:radiation effects; D002455:Cell Division / Q000528:radiation effects; D004247:DNA / Q000528:radiation effects; D004249:DNA Damage; D004260:DNA Repair / Q000502:physiology*; D004307:Dose-Response Relationship, Radiation; D018878:Limb Buds / Q000166:cytology / Q000528:radiation effects*; D051379:Mice; D011509:Proteoglycans / Q000096:biosynthesis; D011740:Pyrimidine Dimers / Q000378:metabolism; D014466:Ultraviolet Rays
chemical_list: D011509:Proteoglycans; D011740:Pyrimidine Dimers; C058406:pyrimidine-pyrimidone dimer; D004247:DNA