Gas chromatographic-mass spectrometric study of lipids and rapid diagnosis of Mycobacterium tuberculosis.
The method suggested is based on the identification of tuberculostearic acid as a Mycobacterium tuberculosis marker. Bacterial culture and sputum of patients with pulmonary tuberculosis and lung diseases of non-tuberculous etiology were investigated. A complete coincidence of the results of bacteriological and gas chromatographic-mass spectrometric (GC-MS) methods used in examining a group of patients with pulmonary tuberculosis was found. GC-MS ion monitoring provides a highly sensitive and rapid technique for the diagnosis of pulmonary tuberculosis.
Mayakova
T I
TI
Irkutsk Institute of Surgery, East-Siberian Scientific Centre, Russian Academy of Medical Sciences, Russian Federation.
Kuznetsova
E E
EE
Kovaleva
M G
MG
Plyusnin
S A
SA
D015415:Biomarkers / Q000032:analysis; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013183:Sputum / Q000737:chemistry; D013229:Stearic Acids / Q000032:analysis*; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis*
D015415:Biomarkers; D013229:Stearic Acids; C026082:tuberculostearic acid