Recovery of mycobacteria from blood in mycobacteria growth indicator tube and Lowenstein-Jensen slant after lysis-centrifugation.
Five hundred five blood samples for culture were processed in the Isolator lysis-centrifugation system and were then inoculated into a Mycobacteria Growth Indicator Tube (MGIT) and onto a Lowenstein-Jensen (L-J) slant. Forty-nine isolates of Mycobacterium avium complex and three isolates of Mycobacterium tuberculosis were recovered from 50 of the blood culture specimens. Forty-five isolates from 43 specimens were recovered in the MGIT, with a mean time to detection of 21 days. Forty-one isolates from 40 specimens were recovered in the L-J slants, and the mean time to detection was 36 days. Nine specimens were positive in the MGIT alone, while seven specimens were positive only in L-J medium.
Hanna
B A
BA
Department of Pathology, New York University School of Medicine, New York 10016, USA.
Walters
S B
SB
Bonk
S J
SJ
Tick
L J
LJ
D017088:AIDS-Related Opportunistic Infections / Q000150:complications / Q000175:diagnosis / Q000382:microbiology; D001431:Bacteriological Techniques; D001769:Blood / Q000382:microbiology*; D002498:Centrifugation; D003470:Culture Media; D005069:Evaluation Studies as Topic; D006461:Hemolysis; D006801:Humans; D015269:Mycobacterium avium Complex / Q000254:growth & development / Q000302:isolation & purification*; D015270:Mycobacterium avium-intracellulare Infection / Q000150:complications / Q000175:diagnosis / Q000382:microbiology; D009169:Mycobacterium tuberculosis / Q000254:growth & development / Q000302:isolation & purification*; D013997:Time Factors; D014397:Tuberculosis, Pulmonary / Q000150:complications / Q000175:diagnosis / Q000382:microbiology
D003470:Culture Media