Characterization of antineutrophil antibodies in patients with neutropenia associated with nutritional copper deficiency.
Six antineutrophil antibody (ANA)-positive patients with copper deficiency were classified into two groups; those with (group A, n = 3) and those without (group B, n = 3) neutropenia. The percent binding of ANA for normal peripheral neutrophils was similar in both groups (83.5 +/- 7.2 vs. 79.1 +/- 10.5%). The percent binding of sera to cultured promyelocytic leukemia cells (HL-60) was increased in group A (from 3.7 +/- 3.2 to 12.2 +/- 2.3%) but not in group B (from 65.3 +/- 21.7 to 40.7 +/- 6.3%) after stimulation of HL-60 with DMSO. The stimulated HL-60 cells expressed CD 16 and CD 11b antigens. In the presence of monoclonal antibody for CD 16, the titer of ANA was nil in group A and unchanged in group B. Thus, ANA of patients with neutropenia may recognize mainly the CD 16 antigen, the Fc gamma receptor III of neutrophils.
Higuchi
S
S
Department of Pediatrics, National Saishunso Hospital, Kumamoto, Japan.
Hirashima
M
M
Nunoi
H
H
Higashi
A
A
Naoe
H
H
Matsuda
I
I
D000293:Adolescent; D000328:Adult; D000368:Aged; D001323:Autoantibodies / Q000097:blood*; D003300:Copper / Q000097:blood / Q000172:deficiency*; D006801:Humans; D015473:Leukemia, Promyelocytic, Acute / Q000276:immunology; D008875:Middle Aged; D009503:Neutropenia / Q000276:immunology*; D009504:Neutrophils / Q000276:immunology*; D009747:Nutritional Physiological Phenomena; D014407:Tumor Cells, Cultured
D001323:Autoantibodies; D003300:Copper