Construction and preliminary characterization of three hemagglutinin mutants of Porphyromonas gingivalis.
Targeted insertional mutagenesis was used to construct hagA, hagB, and hagC hemagglutinin mutants of Porphyromonas gingivalis. pJRD215-derived plasmids containing tetA(Q)2 and portions of the targeted genes were conjugated into P. gingivalis. Interruption of the three loci was confirmed by Southern hybridization, sequencing, reverse transcription-PCR, and microtiter hemagglutination assays. No significant differences in hydrophobicity or coadherence to Actinomyces viscosus were detected between the mutants and the wild-type strain.
Lépine
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Department of Oral Biology, College of Dentistry, University of Florida, Gainesville, Florida 32610, USA.
Ellen
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RP
Progulske-Fox
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A
D001483:Base Sequence; D015139:Blotting, Southern; D006384:Hemagglutination; D006388:Hemagglutinins / Q000235:genetics* / Q000502:physiology; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D016133:Polymerase Chain Reaction; D016966:Porphyromonas gingivalis / Q000235:genetics*; D012313:RNA / Q000032:analysis
D006388:Hemagglutinins; D012313:RNA