Inducibility of RANTES mRNA by IL-1beta in human bronchial epithelial cells is associated with increased NF-kappaB DNA binding activity.
RANTES is a member of a large supergene family of proinflammatory chemokines that seems to play an important role in inflammatory processes. It is produced by many cell types in response to specific stimuli and during inflammatory reactions, but the marked differences in the pattern of induced expression suggest that different control mechanisms regulate transcription of RANTES in various tissue types. This is supported by the presence of a large number of potential binding sites for transcriptional factors in the promoter region of the RANTES gene. Our data indicate that expression of RANTES mRNA induced by IL-1beta in human lung epithelial cells is associated with the activation of the transcriptional factor NF-kappaB.
Manni
A
A
Institute of Experimental Medicine, Milan, Italy.
Kleimberg
J
J
Ackerman
V
V
Bellini
A
A
Patalano
F
F
Mattoli
S
S
D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D001980:Bronchi / Q000276:immunology / Q000378:metabolism*; D002478:Cells, Cultured; D018946:Chemokine CCL5 / Q000235:genetics*; D004247:DNA / Q000235:genetics / Q000378:metabolism*; D015342:DNA Probes / Q000235:genetics; D004848:Epithelium / Q000276:immunology / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology; D008969:Molecular Sequence Data; D016328:NF-kappa B / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics*
D018946:Chemokine CCL5; D015342:DNA Probes; D007375:Interleukin-1; D016328:NF-kappa B; D012333:RNA, Messenger; D004247:DNA