Mechanisms of opsonized HIV entry in normal B lymphocytes.
Using our in vitro model of normal B cell infection that functions with low doses of HIV but requires virus opsonization by seropositive patient serum, and complement, we analyzed what receptors allowed virus entry. Here, we show that HIV infection of B cells occurs through 2 major receptors: the CD4 antigen and the CR1/CR2 complex. These 2 pathways work independently since a complete inhibition of virus entry requires both CD4 and CD21/CD35 blockade on CD4dim tonsillar B cells whereas only the latter is critical on CD4-negative B cells.
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Service de Neurovirologie, CE-FAR, DSV/DRM/SNV, IPSC, Fontenay aux Roses, France.
Gras
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Krzysiek
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Galanaud
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Richard
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Dormont
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D000911:Antibodies, Monoclonal / Q000494:pharmacology; D015703:Antigens, CD / Q000502:physiology; D001402:B-Lymphocytes / Q000276:immunology / Q000821:virology*; D015704:CD4 Antigens / Q000502:physiology*; D002462:Cell Membrane / Q000276:immunology / Q000821:virology; D002648:Child; D003165:Complement System Proteins; D005434:Flow Cytometry; D006678:HIV / Q000502:physiology*; D006679:HIV Seropositivity / Q000097:blood / Q000276:immunology; D006801:Humans; D014066:Palatine Tonsil; D010587:Phagocytosis; D011951:Receptors, Complement / Q000502:physiology*; D015728:Receptors, HIV / Q000502:physiology*
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D015704:CD4 Antigens; D011951:Receptors, Complement; D015728:Receptors, HIV; D003165:Complement System Proteins