Myelin gene expression in glia treated with oligodendroglial trophic factor.
Oligodendroglia synthesize myelin in the CNS. In vitro, oligodendroglia may be identified by the binding of monoclonal antibodies against galactocerebroside, a myelin-specific galactolipid. Oligodendroglial trophic factor is a protein mitogen for cells of the oligodendroglial lineage. When oligodendroglia in cerebral white matter cultures are treated with oligodendroglial trophic factor, galactocerebroside-positive cells undergo mitosis but fail to express the myelin structural proteins, myelin basic protein and proteolipid protein. Oligodendroglia treated with oligodendroglial trophic factor, however, do express 2',3'-cyclic nucleotide 3'-phosphodiesterase and myelin-associated glycoprotein in a manner similar to oligodendroglia treated with platelet-derived growth factor. Oligodendroglial trophic factor, therefore, generates a population of somewhat 'immature' oligodendroglia, which are galactocerebroside, myelin-associated glycoprotein and 2', 3'-cyclic nucleotide 3' phosphodiesterase positive but myelin basic protein and proteolipid protein negative.
Kreider
B Q
BQ
Rutgers, State University, Camden, NJ 08102, USA.
Burns
M M
MM
Grinspan
J B
JB
Morley
M
M
Panek
L A
LA
Shaw
R
R
Pleasure
D
D
D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases / Q000235:genetics; D000818:Animals; D000911:Antibodies, Monoclonal; D001921:Brain / Q000378:metabolism; D002478:Cells, Cultured; D005699:Galactosylceramides / Q000032:analysis; D015870:Gene Expression; D008934:Mitogens / Q000494:pharmacology*; D004676:Myelin Basic Protein / Q000235:genetics; D009185:Myelin Proteins / Q000235:genetics*; D018991:Myelin Proteolipid Protein / Q000235:genetics; D018992:Myelin-Associated Glycoprotein / Q000235:genetics; D009414:Nerve Growth Factors / Q000494:pharmacology*; D009457:Neuroglia / Q000187:drug effects* / Q000378:metabolism*; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
D000911:Antibodies, Monoclonal; D005699:Galactosylceramides; D008934:Mitogens; D004676:Myelin Basic Protein; D009185:Myelin Proteins; D018991:Myelin Proteolipid Protein; D018992:Myelin-Associated Glycoprotein; D009414:Nerve Growth Factors; D010982:Platelet-Derived Growth Factor; D012333:RNA, Messenger; C002454:galactocerebroside; D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases