title: bFGF promotes functional expression of transient outward currents in cultured neonatal rat ventricular cells.
abstract: To investigate the effect of basic fibroblast growth factor (bFGF) on ion channel expressions in developing cardiac cells, a short-term primary culture of ventricular myocytes isolated from day-old newborn rats was established. Functional expressions of the transient outward currents (Ito) were measured by whole-cell voltage-clamp method. Addition of 40 ng/ml bFGF to the serum-free culture medium for 72 hours increased the Ito density without influences on membrane capacitance of the myocytes, an estimate of cell size. bFGF also promoted expressions of the steady-state outward currents. Methyl 2,5-dihydroxycinnamate (3 microM), a tyrosine kinase inhibitor, showed preferential inhibition of the bFGF-stimulated increase in Ito density. These results suggest that bFGF may play a role in regulating ion channel expressions of developing cardiac cells via activation of bFGF receptor tyrosine kinase activity.
authors.affiliation: Department of Circulation, Division of Regulation of Organ Function, Research Institute of Environmental Medicine, Nagoya University, Nagoya 464-01, Japan.
authors.forename: W
authors.initials: W
authors.lastname: Guo
authors.forename: K
authors.initials: K
authors.lastname: Kamiya
authors.forename: J
authors.initials: J
authors.lastname: Toyama
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D002934:Cinnamates / Q000494:pharmacology; D004553:Electric Conductivity; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D007473:Ion Channels / Q000187:drug effects*; D009206:Myocardium / Q000166:cytology*; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors; D051381:Rats; D017208:Rats, Wistar; D012016:Reference Values; D013997:Time Factors
chemical_list: D002934:Cinnamates; D007473:Ion Channels; D016222:Fibroblast Growth Factor 2; C067472:methyl 2,5-dihydroxycinnamate; D011505:Protein-Tyrosine Kinases