PkpA, a novel Phycomyces blakesleeanus serine/threonine protein kinase.
This work reports the cloning and sequencing of pkpA, a gene of the filamentous fungus Phycomyces blakesleeanus, whose expression seems to be coupled to vegetative growth. This gene encodes a putative serine/threonine-specific protein kinase, whose sequence is related to that of the yeast protein STE20, involved in pheromone-response pathways, and to a number of MAPK kinase proteins. However, detailed analysis of the kinase sequence suggests that PkpA is a novel serine/threonine protein kinase that probably participates as an intermediate in an intracellular system controlling nuclear proliferation in P. blakesleeanus.
Ruiz-Pérez
V L
VL
Departamento de Genética y Microbiología, Facultad de Biología, Universidad de Murcia, Spain.
Murillo
F J
FJ
Torres-Martínez
S
S
D000595:Amino Acid Sequence; D001483:Base Sequence; D001665:Binding Sites; D002455:Cell Division; D003001:Cloning, Molecular; D017124:Conserved Sequence / Q000235:genetics; D017931:DNA Primers; D005656:Fungal Proteins / Q000737:chemistry; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D007438:Introns / Q000235:genetics; D008969:Molecular Sequence Data; D010800:Phycomyces / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D017346:Protein Serine-Threonine Kinases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D017421:Sequence Analysis; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic / Q000235:genetics
D017931:DNA Primers; D005656:Fungal Proteins; C098301:PkpA protein, Phycomyces blakesleeanus; D017346:Protein Serine-Threonine Kinases