Release of ferulic acid from wheat bran by a ferulic acid esterase (FAE-III) from Aspergillus niger.
Ferulic acid was efficiently released from a wheat bran preparation by a ferulic acid esterase from Aspergillus niger (FAE-III) when incubated together with a Trichoderma viride xylanase (a maximum of 95% total ferulic acid released after 5 h incubation). FAE-III by itself could release ferulic acid but at a level almost 24-fold lower than that obtained in the presence of the xylanase (2 U). Release of ferulic acid was proportional to the FAE-III concentration between 0.1 U and 1.3 U, but the presence of low levels of xylanase (0.1 U) increased the amount of ferulic acid released 6-fold. Total sugar release was not influenced by the action of FAE-III on the wheat bran, but the rate of release of the apparent end-products of xylanase action (xylose and xylobiose) was elevated by the presence of the esterase. The results show that FAE-III and the xylanase act together to break down feruloylated plant cell-wall polysaccharides to give a high yield of ferulic acid.
Faulds
C B
CB
Department of Food Molecular Biochemistry, Institute of Food Research, UK.
Williamson
G
G
D001234:Aspergillus niger / Q000201:enzymology*; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism*; D002473:Cell Wall / Q000378:metabolism*; D003373:Coumaric Acids / Q000378:metabolism*; D004043:Dietary Fiber / Q000378:metabolism*; D004187:Disaccharides / Q000302:isolation & purification; D005656:Fungal Proteins / Q000378:metabolism*; D008970:Molecular Weight; D014242:Trichoderma / Q000201:enzymology; D043325:Xylan Endo-1,3-beta-Xylosidase; D014994:Xylose / Q000302:isolation & purification; D014995:Xylosidases / Q000378:metabolism
D003373:Coumaric Acids; D004043:Dietary Fiber; D004187:Disaccharides; D005656:Fungal Proteins; D014994:Xylose; C004999:ferulic acid; D002265:Carboxylic Ester Hydrolases; C070196:feruloyl esterase; D014995:Xylosidases; D043325:Xylan Endo-1,3-beta-Xylosidase; C004173:xylobiose