Toxicity of 2,3-dialkyl-1,4-naphthoquinones in rats: comparison with cytotoxicity in vitro.
The short-term toxicities of 2-methyl-1,4-naphthoquinone and a series of 2,3-dialkyl-1,4-naphthoquinones have been determined in rats and compared with their ability to cause oxidative damage to erythrocytes in vitro. In accord with previous results, 2-methyl-1,4-naphthoquinone caused marked oxidative damage to erythrocytes in vitro and haemolytic anaemia in rats. The dialkylnaphthoquinones were also haemolytic agents in vivo, with 2,3-dimethyl-1,4-naphthoquinone being particularly active. Unlike the monoalkyl derivative, however, these substances caused little or no damage to red cells in vitro. The in vivo toxicity of dialkylnaphthoquinones cannot, therefore, be predicted on the basis of in vitro cytotoxicity tests.
Munday
R
R
AgResearch, Ruakura Agricultural Centre, Hamilton, New Zealand.
Smith
B L
BL
Munday
C M
CM
D000743:Anemia, Hemolytic / Q000139:chemically induced / Q000473:pathology; D000818:Animals; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism; D005978:Glutathione / Q000097:blood; D066298:In Vitro Techniques; D007668:Kidney / Q000473:pathology; D008099:Liver / Q000473:pathology; D009285:Naphthoquinones / Q000633:toxicity*; D010084:Oxidation-Reduction; D010108:Oxyhemoglobins / Q000378:metabolism; D051381:Rats; D013154:Spleen / Q000473:pathology; D013481:Superoxides / Q000097:blood; D024483:Vitamin K 3
D009285:Naphthoquinones; D010108:Oxyhemoglobins; D013481:Superoxides; C041498:2,3-dimethyl-1,4-naphthoquinone; D024483:Vitamin K 3; D005978:Glutathione