title: The majority of T lymphocytes are polyclonal during the chronic phase of chronic myelogenous leukemia.
abstract: To clarify the extent of cell lineage involvement in chronic myelogenous leukemia (CML), we investigated the bcr gene rearrangement and clonality using the X-chromosome-linked restriction fragment length polymorphism (RFLP) methylation method in T lymphocytes and granulocytes. We examined the granulocyte and T-cell fractions from the peripheral blood of seven female patients with CML during the chronic phase; patients were heterozygous for RFLPs at the phosphoglycerate kinase (PGK) or the hypoxanthine phosphoribosyltransferase (HPRT) gene. RFLP-methylation analysis of granulocytes demonstrated a monoclonal pattern in six of the seven patients and a rearranged bcr gene in all seven patients. In contrast, T lymphocytes exhibited a polyclonal pattern in six cases; in one case, a faint band was observed following methyl-sensitive enzyme cleavage. The bcr gene analysis in T lymphocytes showed the germline in every case. Our results indicate that the majority of T lymphocytes are polyclonal during the chronic phase of CML and confirm previous reports based on glucose-6-phosphate dehydrogenase, cytogenetic, and bcr rearrangement analyses.
authors.affiliation: Third Department of Internal Medicine, Gunma University School of Medicine, Maebashi, Gunma, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Tsukamoto
authors.forename: M
authors.initials: M
authors.lastname: Karasawa
authors.forename: T
authors.initials: T
authors.lastname: Maehara
authors.forename: K
authors.initials: K
authors.lastname: Okamoto
authors.forename: H
authors.initials: H
authors.lastname: Sakai
authors.forename: T
authors.initials: T
authors.lastname: Naruse
authors.forename: K
authors.initials: K
authors.lastname: Morita
authors.forename: J
authors.initials: J
authors.lastname: Tsuchiya
authors.forename: M
authors.initials: M
authors.lastname: Omine
mesh_terms: D000328:Adult; D014408:Biomarkers, Tumor / Q000032:analysis / Q000235:genetics; D002999:Clone Cells / Q000473:pathology*; D005260:Female; D016044:Fusion Proteins, bcr-abl / Q000032:analysis / Q000235:genetics; D006579:Heterozygote; D006801:Humans; D007041:Hypoxanthine Phosphoribosyltransferase / Q000032:analysis / Q000235:genetics; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics / Q000276:immunology / Q000473:pathology*; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000032:analysis / Q000235:genetics; D010735:Phosphoglycerate Kinase / Q000032:analysis / Q000235:genetics; D012150:Polymorphism, Restriction Fragment Length; D016176:T-Lymphocyte Subsets / Q000473:pathology*
chemical_list: D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins; D007041:Hypoxanthine Phosphoribosyltransferase; D016044:Fusion Proteins, bcr-abl; D010735:Phosphoglycerate Kinase