Expression and purification of recombinant His6-tagged guanine nucleotide dissociation inhibitor and formation of its Rab1 complex.
Peter
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F
Department of Cell Biology, Scripps Research Institute, La Jolla, California 92037, USA.
Nuoffer
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C
Schalk
I
I
Balch
W E
WE
D000818:Animals; D002417:Cattle; D002846:Chromatography, Affinity / Q000379:methods; D002850:Chromatography, Gel / Q000379:methods; D003001:Cloning, Molecular / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D004926:Escherichia coli; D020558:GTP Phosphohydrolases / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism*; D019204:GTP-Binding Proteins / Q000096:biosynthesis* / Q000302:isolation & purification* / Q000378:metabolism; D020730:Guanine Nucleotide Dissociation Inhibitors; D006639:Histidine; D007202:Indicators and Reagents; D008970:Molecular Weight; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000302:isolation & purification; D016324:Sequence Tagged Sites
C086796:GDP dissociation inhibitor 1; D020730:Guanine Nucleotide Dissociation Inhibitors; D007202:Indicators and Reagents; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; D006639:Histidine; D020558:GTP Phosphohydrolases; D019204:GTP-Binding Proteins