Molecular cloning of DNA for inhibin alpha-subunit from equine ovary.
cDNA encoding equine inhibin alpha-subunit precursor protein was isolated from an equine ovarian cDNA library. For screening, the DNA probe was amplified by the RT-PCR using primers designed based on the rat inhibin alpha-subunit cDNA sequence. Out of 1.2 x 10(5) plaques screened, 19 positive clones were isolated, and one of these clones (Eq-alpha-11) contained a complete open reading frame encoding 367 amino acids. The similarity of the deduced amino acid sequences of both equine inhibin alpha-subunit precursor protein and the mature protein were greater than 80% to those of other six mammalian species, including bovine, porcine, ovine, human, rat and mouse. Northern blot analysis revealed that a single major band at 1.5 kb was present in the RNA samples both from equine follicles and testes.
Yamanouchi
K
K
Department of Veterinary Physiology, Veterinary Medical Science, University of Tokyo, Japan.
Yoshida
S
S
Hasegawa
T
T
Ikeda
A
A
Chang
K T
KT
Matsuyama
S
S
Nishihara
M
M
Miyazawa
K
K
Takahashi
M
M
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002417:Cattle; D003001:Cloning, Molecular; D017931:DNA Primers / Q000032:analysis / Q000737:chemistry / Q000235:genetics; D018076:DNA, Complementary / Q000032:analysis / Q000737:chemistry / Q000235:genetics*; D005260:Female; D006736:Horses / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D007265:Inhibins / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D010053:Ovary / Q000737:chemistry* / Q000378:metabolism; D016133:Polymerase Chain Reaction; D051381:Rats; D017386:Sequence Homology, Amino Acid; D012756:Sheep; D013552:Swine; D013737:Testis / Q000737:chemistry / Q000378:metabolism
D017931:DNA Primers; D018076:DNA, Complementary; D007265:Inhibins