cDNA sequence and chromosomal localization of a novel human protein, RBQ-1 (RBBP6), that binds to the retinoblastoma gene product.
We have previously isolated cDNA of a novel protein (RBQ-1, HGMW-approved symbol RBBP6) that binds to the retinoblastoma gene product (pRB). Total nucleotide sequence of the cDNA has now been determined. It encoded a protein of 140 kDa that consists of 948 amino acids and contains multiple repeated sequences like SRS, YRE, and VPPP. The region used for pRB binding was identified on a small region near the C-terminus. We have mapped this gene to 16p11.2-p12 using polymerase chain reaction analysis on a human-hamster hybrid cell panel and chromosomal fluorescence in situ hybridization.
Sakai
Y
Y
Biology Division, National Cancer Center Research Institute, Tokyo, Japan.
Saijo
M
M
Coelho
K
K
Kishino
T
T
Niikawa
N
N
Taya
Y
Y
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D002874:Chromosome Mapping / Q000379:methods; D002885:Chromosomes, Human, Pair 16; D006224:Cricetinae; D018076:DNA, Complementary / Q000302:isolation & purification; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D006822:Hybrid Cells; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D014176:Protein Biosynthesis; D012091:Repetitive Sequences, Nucleic Acid; D016160:Retinoblastoma Protein / Q000378:metabolism*; D044767:Ubiquitin-Protein Ligases
D002352:Carrier Proteins; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D016160:Retinoblastoma Protein; C098584:RBBP6 protein, human; D044767:Ubiquitin-Protein Ligases