Cholecystokinin induced signaling in rat glioma C6 cells.
We investigated the effect of sulphated cholecystokinin octapeptide (CCK-8S) on free intracellular calcium concentration, phosphatidylinositol metabolism, and protein phosphorylation in C6 cells, a rat glioma cell line which was shown to express CCKB type receptors. Increase in [Ca2+]i by both influx across the cell membrane and release from internal stores was demonstrated by utilizing a laser confocal imaging system. Because CCK-8S produced a transient elevation of inositol triphosphate level participation of InsP3 in calcium signaling in C6 cells is very likely. Protein kinase C seems to be involved in CCK-8S induced signaling in rat glioma C6 cells as demonstrated by using in vivo phosphorylation experiments.
Kaufmann
R
R
Max Planck Gesellschaft, Research Unit Pharmacological Haemostaseology, Friedrich Schiller University, Germany.
Schöneberg
T
T
Lindschau
C
C
Haller
H
H
Ott
T
T
D000818:Animals; D002118:Calcium / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005910:Glioma / Q000378:metabolism*; D007295:Inositol Phosphates / Q000378:metabolism; D010716:Phosphatidylinositols / Q000378:metabolism; D010750:Phosphoproteins / Q000378:metabolism; D010766:Phosphorylation; D011493:Protein Kinase C / Q000378:metabolism; D051381:Rats; D015398:Signal Transduction / Q000187:drug effects*; D012844:Sincalide / Q000031:analogs & derivatives* / Q000494:pharmacology; D014407:Tumor Cells, Cultured
C090019:8-sulfocholecystokinin octapeptide; D007295:Inositol Phosphates; D010716:Phosphatidylinositols; D010750:Phosphoproteins; D011493:Protein Kinase C; D012844:Sincalide; D002118:Calcium