title: [Immunoenzymatic assay for serodiagnosis of Helicobacter pylori infections].
abstract: Enzyme-linked immunosorbent assays (ELISA) have been developed to diagnose H. pylori infection. However, the methods are not standardized. We therefore evaluated the sensitivity of ELISA developed in our laboratory in a study population comprising 38 patients with dyspeptic symptoms and 41 healthy children under 10 years. The assays for immunoglobulin G used a pool of sonicated isolates of H. pylori from five patients with dyspeptic symptoms. Serum levels IgG antibodies to cell sonicates of H. pylori were significantly higher (p < 0.001) in patients with positive culture than in the control group. Cutoff score of 0.231 for positive results was determined with mean + 3ds of the healthy group analyzed. On the basis of this survey, we conclude that a serum dilution of 1:300 and 5 micrograms/ml of antigen, are the optimal condition for rapid screening with high sensitivity of 92% of symptomatic patients for the presence of H. pylori.
authors.affiliation: Facultad de Medicina, U.C.V., *Instituto de Oncologia y Hematologia, M.S.A.S., Hospital J.M. Vargas de Caracas, Venezuela.
authors.forename: E P
authors.initials: EP
authors.lastname: Chacon
authors.forename: M
authors.initials: M
authors.lastname: Correnti
authors.forename: N
authors.initials: N
authors.lastname: Salma
authors.forename: N
authors.initials: N
authors.lastname: Serrano
authors.forename: R
authors.initials: R
authors.lastname: Piñero
authors.forename: M E
authors.initials: ME
authors.lastname: Cavazza
authors.forename: M I
authors.initials: MI
authors.lastname: Urrestarazu
mesh_terms: D000942:Antigens, Bacterial / Q000097:blood; D002648:Child; D002675:Child, Preschool; D004381:Duodenal Ulcer / Q000382:microbiology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D016481:Helicobacter Infections / Q000175:diagnosis*; D016480:Helicobacter pylori / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007223:Infant; D008297:Male; D012680:Sensitivity and Specificity; D012698:Serologic Tests
chemical_list: D000942:Antigens, Bacterial; D007074:Immunoglobulin G