[Histological study for endothelial seeded vascular prostheses-experimental study on caine].
Endothelial seeded graft has been investigated for a small-caliber vascular graft. In this report, we established endothelial lining onto ultrafiber graft by the filtration method and implanted it to caine abdominal aorta. The grafts were harvested in 1 to 4 months and examined endothelialization and subendothelial structure by light microscopy and electron microscopy. The cell counts of pre and post flush through the graft were calculated. pre:9.8 +/- 10(6)/ml post: 1.2 +/- 0.60 x 10(6)/ml trap ratio: 86.4 +/- 4.6%. The thickness of inner capsule of seeding part were significantly low than that of control part. But there were no significant difference of inner capsule of anastomosis between seeding part and control part. In the seeded part endothelial lining and well incorporation were observed by light microscopy. Electronmicroscopic study revealed that seeded endothelium had desmosome and secreted a basement membrane. In the control part there was only small endothelial inoculation and thrombus formation which caused stenosis of graft. The filtration method revealed the efficacy to seed on to vascular prosteses for long time period. The seeded graft had antithrobogenity and well incorporation.
Egami
J
J
Second Department of Surgery, Faculty of Medicine, University of Tokyo, Japan.
D000818:Animals; D001012:Aorta, Abdominal / Q000601:surgery; D001807:Blood Vessel Prosthesis / Q000379:methods*; D002452:Cell Count; D004285:Dogs; D004730:Endothelium, Vascular / Q000166:cytology* / Q000637:transplantation*; D011474:Prosthesis Design