L-fucose residues on cellulose-based dialysis membranes: quantification of membrane-associated L-fucose and analysis of specific lectin binding.
Contact of mononuclear human leukocytes with cellulose dialysis membranes may result in complement-independent cell activation, i.e. enhanced synthesis of cytokines, prostaglandins and an increase in beta 2-micro-globulin synthesis. Cellular contact activation is specifically inhibited by the monosaccharide L-fucose suggesting that dialysis membrane associated L-fucose residues are involved in leukocyte activation. In this study we have detected and quantitated L-fucose on commercially-available cellulose dialysis membranes using two approaches. A sensitive enzymatic fluorescence assay detected L-fucose after acid hydrolysis of flat sheet membranes. Values ranged from 79.3 +/- 3.6 to 90.2 +/- 5.0 pmol cm-2 for Hemophan or Cuprophan respectively. Enzymatic cleavage of terminal alpha-L-fucopyranoses with alpha-L-fucosidase yielded 7.7 +/- 3.3 pmol L-fucose per cm2 for Cuprophan. Enzymatic hydrolysis of the synthetic polymer membranes AN-69 and PC-PE did not yield detectable amounts of L-fucose. In a second approach, binding of the fucose specific lectins of Lotus tetragonolobus and Ulex europaeus (UEAI) demonstrated the presence of biologically accessible L-fucose on the surface of cellulose membranes. Specific binding was observed with Cuprophan, and up to 2.6 +/- 0.3 pmol L-fucose per cm2 was calculated to be present from Langmuir-type adsorption isotherms. The data presented are in line with the hypothesis that surface-associated L-fucose residues on cellulose dialysis membranes participate in leukocyte contact activation.
Meissner
C
C
Institute of Immunology, Heidelberg, Germany.
Deppisch
R
R
Hug
F
F
Schulze
M
M
Ritz
E
E
Ludwig
H
H
Hänsch
G
G
D000180:Acrylic Resins; D000181:Acrylonitrile / Q000031:analogs & derivatives; D000327:Adsorption; D001672:Biocompatible Materials; D002482:Cellulose / Q000031:analogs & derivatives; D003956:Dialysis; D005643:Fucose / Q000032:analysis*; D006801:Humans; D006868:Hydrolysis; D007700:Kinetics; D037102:Lectins; D008567:Membranes, Artificial; D009504:Neutrophils / Q000276:immunology / Q000502:physiology*; D010835:Phytohemagglutinins; D011108:Polymers
D000180:Acrylic Resins; D001672:Biocompatible Materials; D037102:Lectins; D008567:Membranes, Artificial; D010835:Phytohemagglutinins; D011108:Polymers; C027112:fucose-binding lectin; C054645:Hemophan; D005643:Fucose; C049723:polycarbonate-polyether copolymer; C016892:AN-69; D002482:Cellulose; C009865:cuprammonium cellulose; D000181:Acrylonitrile