title: Effects of kanamycin ototoxicity and hair cell regeneration on the DC endocochlear potential in adult chickens.
abstract: High doses of aminoglycoside antibiotics cause massive damage to the avian basilar papilla. The resulting functional loss could conceivably arise from the reduction in the DC endocochlear potential (EP) due to impairment of the tegmentum vasculosum (TV) or to shunting of current through the damaged sensory epithelium. To test this hypothesis, the EP was measured in adult chickens after destroying hair cells in the basal half of the cochlea with a high dose (400 mg/kg per day for 10 days) of kanamycin (KM). KM treatment caused an increase in the steady-state EP from +18.1 to +23.3 mV and a decrease in the magnitude of the negative EP from -42.0 to -19.2 mV. The EP showed almost no change between 1 and 2 days and 1 week post-KM treatment. After 4 weeks of recovery, most hair cells had regenerated; however, the steady-state EP was still elevated by 13% and the negative EP was depressed by 37%. These results suggest that functional loss as shown by the large reduction in cochlear microphonic (CM) and the elevated thresholds of compound action potential (CAP) following KM treatment is not due to a reduction in the EP but may arise from functional deficits in the hair cells and/or the auditory nerve.
authors.affiliation: Department of Communicative Disorders and Sciences, State University of New York at Buffalo 14214, USA.
authors.forename: L
authors.initials: L
authors.lastname: Chen
authors.forename: P G
authors.initials: PG
authors.lastname: Trautwein
authors.forename: K
authors.initials: K
authors.lastname: Miller
authors.forename: R J
authors.initials: RJ
authors.lastname: Salvi
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000633:toxicity*; D002645:Chickens; D003051:Cochlea / Q000187:drug effects* / Q000293:injuries / Q000503:physiopathology; D003055:Cochlear Microphonic Potentials / Q000187:drug effects* / Q000502:physiology; D005072:Evoked Potentials, Auditory / Q000187:drug effects / Q000502:physiology; D005260:Female; D006198:Hair Cells, Auditory / Q000187:drug effects* / Q000503:physiopathology; D007612:Kanamycin / Q000633:toxicity*; D012038:Regeneration; D013997:Time Factors; D000159:Vestibulocochlear Nerve / Q000187:drug effects / Q000503:physiopathology; D061285:Vestibulocochlear Nerve Injuries
chemical_list: D000900:Anti-Bacterial Agents; D007612:Kanamycin