Amphetamine and methamphetamine determination in urine by reversed-phase high-performance liquid chromatography with sodium 1,2-naphthoquinone 4-sulfonate as derivatizing agent and solid-phase extraction for sample clean-up.
A rapid method is described for the identification and determination of amphetamine and methamphetamine in human urine samples by liquid chromatography with UV-Vis detection. The samples were transferred onto a C18 solid-phase extraction column and chromatographed on a Hypersil ODS RP C18, 5 microns (250 x 4 mm I.D.) with an acetonitrile-water elution gradient containing propylamine. Under these conditions, the amines are eluted with a short retention time. The procedure has been applied to the determination of amphetamine and methamphetamine in the range 0.3-4.0 micrograms/ml in spiked urine samples. The detection limits at 280 nm were 4 and 2 ng/ml for amphetamine and methamphetamine, respectively. The intra-day and inter-day precision and accuracy of the method were studied.
Molíns Legua
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Departamento de Química Analítica, Facultad de Química, Universidad de Valencia, Burjassot (Valencia), Spain.
Campíns Falcó
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Sevillano Cabeza
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D000661:Amphetamine / Q000652:urine*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D007202:Indicators and Reagents; D008694:Methamphetamine / Q000652:urine*; D009285:Naphthoquinones; D015203:Reproducibility of Results; D013055:Spectrophotometry, Infrared
D007202:Indicators and Reagents; D009285:Naphthoquinones; C011942:1,2-naphthoquinone-4-sulfonate; D008694:Methamphetamine; D000661:Amphetamine