Autoantigenicity of human sperm: molecular identities of sperm antigens recognized by sera of immunoinfertile men in the immunoprecipitation procedure.
Autoantigenicity of human sperm as related to immunoinfertility was investigated using the immunoprecipitation procedure. Sera from immunoinfertile men specifically immunoprecipitated six specific bands of 135, 95, and 65 kD and double bands of 47, 41, and 23 kD that were not recognized by the sera from fertile men. Of these six specific bands, three bands of 65-, 47-, and 23-kD molecular identities were recognized by > 60% of immunoinfertile sera, and 47 and 23 kD may correspond to the dimeric and monomeric forms of FA-1 antigen, respectively. Seminal plasma from immunoinfertile men specifically recognized three specific bands of 95, 37, and 16 kD; only the 95-kD band was also recognized with the immunoinfertile sera. These specific antigens may find applications in immunocontraception and immunoinfertility in humans.
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Department of Obstetrics and Gynecology, Albert Einstein College of Medicine, Bronx, New York 10461, USA.
Gateva
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Morte
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D000328:Adult; D001323:Autoantibodies / Q000032:analysis* / Q000097:blood / Q000276:immunology; D001324:Autoantigens / Q000032:analysis* / Q000097:blood / Q000276:immunology*; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D007248:Infertility, Male / Q000097:blood / Q000276:immunology*; D008297:Male; D011233:Precipitin Tests; D013094:Spermatozoa / Q000276:immunology*
D001323:Autoantibodies; D001324:Autoantigens; D007106:Immune Sera