title: Genetically modified Chinese hamster ovary (CHO) cells for studying the genotoxicity of heterocyclic amines from cooked foods.
abstract: We have developed metabolically competent Chinese hamster ovary (CHO) cells to evaluate the genotoxicity associated with heterocyclic amines, such as those that are present in cooked foods. Into repair-deficient UV5 cells we introduced cDNAs for expressing cytochrome P450IA2 and acetyltransferases. We then genetically reverted these transformed lines to obtain matched metabolically competent repair-deficient/proficient lines. For a high mutagenic response, we find a requirement for acetyltransferase with 2-amino-3-methylimidazo[4,5-f]quinoline (IQ) but not with 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP). This system allows for both quantifying mutagenesis and analyzing the mutational spectra produced by heterocyclic amines.
authors.affiliation: Biology and Biotechnology Research Program, Lawrence Livermore National Laboratory, Livermore, CA 94551-0808, USA.
authors.forename: L H
authors.initials: LH
authors.lastname: Thompson
authors.forename: R W
authors.initials: RW
authors.lastname: Wu
authors.forename: J S
authors.initials: JS
authors.lastname: Felton
mesh_terms: D000818:Animals; D001711:Biotransformation; D016466:CHO Cells; D006224:Cricetinae; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis; D007093:Imidazoles / Q000378:metabolism / Q000633:toxicity*; D009153:Mutagens / Q000633:toxicity*; D009154:Mutation; D011804:Quinolines / Q000633:toxicity*
chemical_list: D007093:Imidazoles; D009153:Mutagens; D011804:Quinolines; C029216:2-amino-3-methylimidazo(4,5-f)quinoline; D003577:Cytochrome P-450 Enzyme System; C049584:2-amino-1-methyl-6-phenylimidazo(4,5-b)pyridine