Attenuation of reperfusion injury by the antioxidant n-propyl gallate.
We studied the effects of n-propyl gallate (n-PG) on Langendorff preparations of isolated rat hearts. Perfusion of the hearts with Krebs-Henseleit (KH) solution containing 20 microM n-PG did not cause a statistically significant change in either left ventricular systolic pressure (LVSP), end-diastolic pressure (LVEDP), developed pressure (LVDP), or heart rate (HR), indicating that n-PG has little acute myocardial toxicity. The effects of n-PG on the reperfused and ischemic myocardium were tested in hearts subjected to 15-min global P4 the ischemic hearts with KH buffer resulted in the recovery of the LVDP to 66 +/- 7% (mean +/- SEM, n = 11) and the recovery of the rate-pressure product (RPP) to 65 +/- 7% of their preischemic values. The LVEDP of the reperfused hearts was 30 +/- 5 mm Hg as compared with the preischemic LVEDP of 5.2 +/- 0.9 mm Hg. The difference between the coronary flow rate of the preischemic hearts (15.4 +/- 0.8 ml/min) and the reperfused hearts (13.9 +/- 0.9 ml/min) was not statistically significant (p > 0.05). Addition of n-PG, at the time of reperfusion, resulted in with KH buffer containing 20 microM n-PG had LVEDP of 6.2 +/- 0.4 mm Hg, and both LVDP and RPP recovered to 92 +/- 4% of the preischemic control.(ABSTRACT TRUNCATED AT 250 WORDS)
Bhatnagar
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A
Department of Physiology and Biophysics, University of Texas Medical Branch, Galveston, USA.
D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D001794:Blood Pressure / Q000187:drug effects; D002021:Buffers; D003326:Coronary Circulation / Q000187:drug effects; D004195:Disease Models, Animal; D006339:Heart Rate / Q000187:drug effects; D066298:In Vitro Techniques; D009200:Myocardial Contraction / Q000187:drug effects; D015428:Myocardial Reperfusion Injury / Q000517:prevention & control*; D011435:Propyl Gallate / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley; D016277:Ventricular Function, Left / Q000187:drug effects
D000975:Antioxidants; D002021:Buffers; D011435:Propyl Gallate