Sonodynamically induced antitumor effect of pheophorbide a.
The sonodynamically induced antitumor effect of pheophorbide a (Ph-a) was investigated. Both in vitro and in vivo effects on sarcoma 180 were tested in combination with ultrasound at 2 MHz. The rate of ultrasonically induced cell damage in air-saturated suspension was enhanced by twice with 80 microM Ph-a. This enhancement was significantly inhibited by histidine, which may suggest it was mediated by ultrasonically induced oxidation. For mice, 5 mg/kg Ph-a was administered before the insonation, and ultrasound stopped the tumor growth at an intensity with which ultrasound alone showed only a slight antitumor effect.
Umemura
K
K
School of Pharmaceutical Sciences, Toho University, Chiba, Japan.
Yumita
N
N
Nishigaki
R
R
Umemura
S
S
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002734:Chlorophyll / Q000031:analogs & derivatives* / Q000494:pharmacology; D006639:Histidine / Q000494:pharmacology; D008297:Male; D008353:Mannitol / Q000494:pharmacology; D051379:Mice; D008813:Mice, Inbred ICR; D011838:Radiation-Sensitizing Agents / Q000494:pharmacology*; D012510:Sarcoma 180 / Q000188:drug therapy* / Q000628:therapy*; D013482:Superoxide Dismutase / Q000494:pharmacology; D014464:Ultrasonic Therapy
D000970:Antineoplastic Agents; D011838:Radiation-Sensitizing Agents; D002734:Chlorophyll; D008353:Mannitol; D006639:Histidine; D013482:Superoxide Dismutase; C032623:pheophorbide a