On-column isomerization of sugars during high-performance liquid chromatography: analysis of the elution profile.
Four monosaccharides (glucose, galactose, mannose and fructose) and one disaccharide (maltose) were subjected to high-performance liquid chromatography with UV or refractive index detection. Various profiles such as broad, tailed and splitted peaks were produced, depending on column temperature and eluent flow-rate because these saccharides underwent isomerization. In contrast, alpha-methylglucoside, a non-converting derivative, always produced a sharp peak. By analyzing these profiles kinetic constants of the isomerization were obtained and compared with the literature data.
Nishikawa
T
T
Department of Clinical Pathology, School of Medicine, Kitasato University, Kanagawa, Japan.
Suzuki
S
S
Kubo
H
H
Ohtani
H
H
D002241:Carbohydrates / Q000032:analysis* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D007536:Isomerism; D007700:Kinetics; D008320:Maltose / Q000032:analysis / Q000737:chemistry; D009005:Monosaccharides / Q000032:analysis / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet
D002241:Carbohydrates; D009005:Monosaccharides; D008320:Maltose