Effect of sodium valproate on renal cell brush-border enzymes in rats.
The effect of sodium valproate (200 mg/kg body weight) on renal cells was investigated during a 10-day trial, by determining the catalytic activities of alanine aminopeptidase, gamma-glutamyltransferase and alkaline phosphatase in the membrane cell brush-border of the renal proximal tubules. Four days after the administration of sodium valproate, a significant increase was observed in the volume of urine (9.2 +/- 4.2 ml/18-h volume; control group, 3.06 +/- 1.8 ml/18-h volume), and in the catalytic activities of gamma-glutamyltransferase (2.69 times that measured in the control group) and alkaline phosphatase (3.02 times that measured in the control group) in urine. After prolonged treatment the urine became alkaline (pH = 10.5 on day 10), thereby excluding alanine aminopeptidase as a useful indicator of renal cell changes following the administration of sodium valproate. The activities of alkaline phosphatase and gamma-glutamyltransferase in isolated vesicles of renal brush-border membrane cells were significantly increased (p < 0.05) and decreased, respectively. On the basis of the results obtained, we believe that the determination of the catalytic activities of gamma-glutamyltransferase and alkaline phosphatase in urine might prove useful for the follow-up of the renal cell state during therapy with sodium valproate.
Cepelak
I
I
Institute of Medical Biochemistry, School of Pharmacy and Biochemistry, University of Zagreb, Croatia.
Rekić
B
B
Juretić
D
D
Mandusić
A
A
D000469:Alkaline Phosphatase / Q000187:drug effects / Q000378:metabolism* / Q000652:urine; D000818:Animals; D000927:Anticonvulsants / Q000494:pharmacology*; D018826:CD13 Antigens / Q000187:drug effects / Q000378:metabolism* / Q000652:urine; D002384:Catalysis; D007687:Kidney Tubules, Proximal / Q000187:drug effects / Q000201:enzymology* / Q000648:ultrastructure*; D008297:Male; D008871:Microvilli / Q000201:enzymology; D051381:Rats; D011916:Rats, Inbred F344; D014635:Valproic Acid / Q000494:pharmacology*; D005723:gamma-Glutamyltransferase / Q000187:drug effects / Q000378:metabolism* / Q000652:urine
D000927:Anticonvulsants; D014635:Valproic Acid; D005723:gamma-Glutamyltransferase; D000469:Alkaline Phosphatase; D018826:CD13 Antigens