Platelet activating factor and lyso-phosphatidylcholines from strawberry.
Lipids from strawberry fruits, leaves, achenes and pollen were separated into classes by TLC, purified by HPLC and tested for biological activity. A lipid fraction from fruits with the same chromatographic behaviour as authentic platelet activating factor (PAF) showed identical biological activity, namely, dose-dependent aggregation of washed rabbit platelets, inhibition of aggregation by CV 3988, platelet desensitization to PAF and vice versa, and loss of activity by alkaline hydrolysis and recovery of activity by reacetylation. The presence of PAF was confirmed by FAB mass spectrometry. Lyso-phosphatidylcholines, including lyso-PAF, were also found in all the plant parts tested.
Calligerou
M
M
Department of Chemistry (Biochemistry), University of Athens, Zografou, Greece.
Siafaka-Kapadai
A
A
Galanopoulou
D
D
Weintraub
S T
ST
Mavri-Vavayanni
M
M
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D005638:Fruit; D066298:In Vitro Techniques; D008244:Lysophosphatidylcholines / Q000737:chemistry / Q000302:isolation & purification*; D010742:Phospholipid Ethers / Q000494:pharmacology; D018515:Plant Leaves; D010972:Platelet Activating Factor / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D010974:Platelet Aggregation / Q000187:drug effects*; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology; D011058:Pollen; D011817:Rabbits; D016339:Spectrometry, Mass, Fast Atom Bombardment
D008244:Lysophosphatidylcholines; D010742:Phospholipid Ethers; D010972:Platelet Activating Factor; D010975:Platelet Aggregation Inhibitors; C037913:CV 3988