RGDFAP: platelet aggregation inhibitory and profibrinolytic hybrid peptide (RGDF coupled with the carboxy terminal part of alpha 2-antiplasmin) enhances plasminogen binding to platelets.
As published in a recent issue of Blood Coagulation and Fibrinolysis, the hybrid peptide RGDFAP, composed of RGDF (Arg-Gly-Asp-Phe) coupled to a synthetic peptide residue of the carboxy terminal part of antiplasmin (AP26) inhibited platelet activation and augmented plasmin generation and in vitro fibrin clot lysis. This peptide contains an RGD motif which provides linkage to platelet GP IIb-IIIa. The antiplasmin part of the molecule may attach free plasminogen, which in turn increases the amount of platelet surface bound plasminogen, probably yielding enhanced lytic action at the site of thrombus formation. This hypothesis was investigated and confirmed by the results of platelet-plasminogen binding assays, using FITC-labelled antiplasmin antibodies and radioligand binding analysis. Increased platelet-linked plasminogen was detected by a chromogenic method, along with the acceleration of in vitro lysis of platelet-rich clots in the presence of RGDFAP peptide.
Udvardy
M
M
2nd Department of Medicine, Debrecen University Medical School, Hungary.
Schwartzott
D
D
Jackson
K
K
Posan
E
E
Rak
K
K
McKee
P A
PA
D000595:Amino Acid Sequence; D001792:Blood Platelets / Q000187:drug effects* / Q000378:metabolism*; D005342:Fibrinolysis / Q000187:drug effects*; D005434:Flow Cytometry; D016650:Fluorescein-5-isothiocyanate; D006801:Humans; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000737:chemistry; D010455:Peptides / Q000494:pharmacology*; D010958:Plasminogen / Q000378:metabolism*; D010974:Platelet Aggregation / Q000187:drug effects*; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*; D055503:Protein Multimerization; D000979:alpha-2-Antiplasmin / Q000737:chemistry
C098253:Arg-Gly-Asp-Phe-Gly-Gly-Gly-Gly-AP26; D009842:Oligopeptides; D010455:Peptides; D010975:Platelet Aggregation Inhibitors; D000979:alpha-2-Antiplasmin; C084961:arginyl-glycyl-aspartyl-phenylalanine; D010958:Plasminogen; D016650:Fluorescein-5-isothiocyanate