Human CD34+ cell EST database: single-pass sequencing of 402 clones from a directional cDNA library.
A directional cDNA library was constructed from human CD34+ cells, and single-pass DNA sequence analysis was used to sequence 402 randomly picked cDNA clones. Sequence homology searches against the GenBank/EMBL databases revealed that 144 clones (35.8%) represented new genes, whereas 39 clones (9.7%) matched the partial sequences of cDNA clones (expressed sequence tags [ESTs]) from cDNA libraries from other tissues or cells. One hundred three (25.6%) of the clones were highly homologous or identical to genes of known function. 18.6% of the clones were ribosomal and 6 % were mitochondrial sequences. Seventeen (4.2%) of the clones were coding for Alu or L1 repetitive sequences. Our results suggest that random single-pass sequencing of cDNA clones provides a rapid way to obtain a profile of genes expressed in CD34+ cells and to identify new and potentially interesting genes.
Yang
Y
Y
Division of Hematology, University of Washington, Seattle, USA.
Peterson
K R
KR
Stamatoyannopoulos
G
G
Papayannopoulou
T
T
D018952:Antigens, CD34 / Q000032:analysis*; D001483:Base Sequence; D001854:Bone Marrow Cells; D018076:DNA, Complementary / Q000235:genetics; D016208:Databases, Factual; D015870:Gene Expression; D015723:Gene Library; D005796:Genes; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000737:chemistry / Q000502:physiology*; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid
D018952:Antigens, CD34; D018076:DNA, Complementary; D012333:RNA, Messenger