High pressure represses expression of the malB operon in Escherichia coli.
The formation of plaques by lambda phage in Escherichia coli was prevented by elevated hydrostatic pressure; phage plaques were not detected at 30 MPa. Furthermore, using promoter fragments derived from the malB operon, we showed that gene expression initiated from both promoters (malK-lamB and malEFG) was repressed by elevated hydrostatic pressure. Our findings suggest that high pressure affects gene expression directed by the malB regulatory interval, and this may cause a decrease in the quantities of lambda receptor protein, LamB.
Sato
T
T
DEEPSTAR group, Japan Marine Science and Technology Center, Yokosuka, Japan.
Nakamura
Y
Y
Nakashima
K K
KK
Kato
C
C
Horikoshi
K
K
D001274:Atmospheric Pressure; D001435:Bacteriophages / Q000254:growth & development; D004926:Escherichia coli / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial / Q000502:physiology*; D017930:Genes, Reporter / Q000502:physiology; D009876:Operon / Q000502:physiology*; D011401:Promoter Regions, Genetic