Ultrastructural modifications and changes in the expression of hormonal genes produced by indomethacin in the anterior pituitary gland of the rat.
Indomethacin decreases the level of prolactin (50%) and growth hormone (70%) mRNA in the anterior pituitary gland of the rat. Actin mRNA increases (59%). Ultrastructurally there is a decrease in the number of secretory granules. Indomethacin also prevents the increase in prolactin secretory granules produced by the administration of estradiol. The results indicate that indomethacin inhibits hormonal synthesis in the APG at a transcriptional level. This effect appears selective because mRNA level for actin synthesis in the pituitary gland was higher than in nontreated rats.
Romano
L A
LA
Department of Endocrinology, Fundación CIMAE, Buenos Aires, Argentina.
Rivolta
C
C
Machiavelli
G A
GA
Burdman
J A
JA
D000199:Actins / Q000235:genetics; D000818:Animals; D018076:DNA, Complementary; D004958:Estradiol / Q000494:pharmacology; D015870:Gene Expression / Q000187:drug effects; D013006:Growth Hormone / Q000235:genetics*; D007213:Indomethacin / Q000494:pharmacology*; D008297:Male; D008854:Microscopy, Electron; D010903:Pituitary Gland, Anterior / Q000166:cytology / Q000502:physiology* / Q000648:ultrastructure; D011388:Prolactin / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar
D000199:Actins; D018076:DNA, Complementary; D012333:RNA, Messenger; D004958:Estradiol; D011388:Prolactin; D013006:Growth Hormone; D007213:Indomethacin