Automatic gas chromatographic determination of the high-density-lipoprotein cholesterol and total cholesterol in serum.
A new analytical method that combines on-line precipitation-filtration, enzymatic hydrolysis, extraction and gas chromatography was developed for the determination of total cholesterol and high-density-lipoprotein cholesterol in human serum. Very-low-density lipoprotein, intermediate-density lipoprotein and low-density lipoprotein are precipitated with sodium phosphotungstate and magnesium chloride; then, the serum is continuously filtered and unprecipitated high-density-lipoprotein cholesterol is enzymatically hydrolyzed and finally determined as cholesterol by gas chromatography. Total cholesterol is also determined by direct introduction of the serum into the proposed system. The proposed method was validated by analyzing a lipid control serum with certified contents of high-density-lipoprotein cholesterol and total cholesterol. The results obtained were consistent with the certified contents.
Cárdenas
M S
MS
Department of Analytical Chemistry, Faculty of Sciences, University of Córdoba, Spain.
Ballesteros
E
E
Gallego
M
M
Valcárcel
M
M
D002784:Cholesterol / Q000097:blood*; D008076:Cholesterol, HDL / Q000097:blood*; D002849:Chromatography, Gas; D004800:Enzymes, Immobilized; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007202:Indicators and Reagents; D015636:Magnesium Chloride; D010772:Phosphotungstic Acid; D002787:Sterol Esterase; D013696:Temperature
D008076:Cholesterol, HDL; D004800:Enzymes, Immobilized; D007202:Indicators and Reagents; D015636:Magnesium Chloride; D010772:Phosphotungstic Acid; D002784:Cholesterol; D002787:Sterol Esterase