title: Localization and physical mapping of the prostate-specific membrane antigen (PSM) gene to human chromosome 11.
abstract: The prostate-specific membrane antigen (PSM) was identified by the monoclonal antibody 7E11-C5.3, which was raised against the human prostatic carcinoma cell line LNCaP (3). The PSM antigen is expressed by normal, neoplastic, and metastatic prostatic tissues. The 2.65-kb cDNA encoding the 100-kDa PSM glycoprotein was cloned from LNCaP cells (4). Studies have shown that the expression of PSM is tissue-specific (5). In the present study monochromosomal somatic cell hybrids were used to localize the PSM gene to human chromosome 11. Using this information, initial mapping studies identified two potential PSM gene loci at 11p11.1-p13 and 11q14. Further high-stringency analysis using cosmid probes identified the 11q14 region as the location of the PSM gene.
authors.affiliation: Brady Urological Institute, Johns Hopkins Medical School, Baltimore, Maryland 21218, USA.
authors.forename: C W
authors.initials: CW
authors.lastname: Rinker-Schaeffer
authors.forename: A L
authors.initials: AL
authors.lastname: Hawkins
authors.forename: S L
authors.initials: SL
authors.lastname: Su
authors.forename: R S
authors.initials: RS
authors.lastname: Israeli
authors.forename: C A
authors.initials: CA
authors.lastname: Griffin
authors.forename: J T
authors.initials: JT
authors.lastname: Isaacs
authors.forename: W D
authors.initials: WD
authors.lastname: Heston
mesh_terms: D000951:Antigens, Neoplasm / Q000235:genetics*; D000954:Antigens, Surface / Q000235:genetics*; D015139:Blotting, Southern; D002874:Chromosome Mapping / Q000379:methods; D002880:Chromosomes, Human, Pair 11; D043425:Glutamate Carboxypeptidase II; D006801:Humans; D006822:Hybrid Cells; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D008297:Male
chemical_list: D000951:Antigens, Neoplasm; D000954:Antigens, Surface; C473848:FOLH1 protein, human; D043425:Glutamate Carboxypeptidase II