Determination of salbutamol enantiomers in human plasma and urine by chiral high-performance liquid chromatography.
Enantiomers of salbutamol were directly separated (Rs = 1.16) and quantitated at therapeutic concentrations after solid-phase extraction from human plasma and urine by normal-phase high-performance liquid chromatography on a chiral column with fluorescence detection. The assay was linear for each enantiomer between 1.25 and 500 ng ml-1 and had a minimum limit of detection of 250 pg ml-1. A 3-ml plasma or 1-ml urine sample was required for quantitation at therapeutic doses. Inter-day variation was 5.0% for S-(+)- and 6.5% for R-(-)-salbutamol. The assay was used to compare enantioselective disposition after single doses of racemate by the intravenous, oral and rectal routes.
Boulton
D W
DW
School of Pharmacy, University of Otago, Dunedin, New Zealand.
Fawcett
J P
JP
D000284:Administration, Oral; D000285:Administration, Rectal; D000318:Adrenergic beta-Agonists / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics / Q000652:urine*; D000328:Adult; D000420:Albuterol / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics / Q000652:urine*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007275:Injections, Intravenous; D008297:Male; D012044:Regression Analysis; D013050:Spectrometry, Fluorescence; D013237:Stereoisomerism
D000318:Adrenergic beta-Agonists; D000420:Albuterol