Prothrombin conversion intermediate effectively neutralizes toxic levels of hirudin.
Meizothrombin, the stable intermediate product of ecarin-induced prothrombin conversion, was investigated for its ability to bind hirudin in blood. After in vitro pre-incubation of rat plasma with ecarin, the prolongation of the thrombin time caused by hirudin was reduced. The extent of hirudin neutralization was found to be dependent on the duration of incubation with ecarin. In vivo, after bilateral nephrectomy in Wistar rats and following administration of hirudin at a dose of 1 or 5 mg/kg, the blood level of hirudin remained constant after 2 h. After infusion of ecarin following hirudin administration, the hirudin blood level dropped sharply, reaching significantly reduced values, and bleeding stopped. Platelet count and fibrinogen level in plasma remained unchanged in the experiments using ecarin-induced prothrombin conversion intermediate generation. It is concluded that meizothrombin, a naturally occurring prothrombin conversion intermediate, provides an effective agent to neutralize toxic blood levels of hirudin.
Nowak
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G
Max-Planck-Gesellschaft e. V., Research Unit, Pharmacological Haemostaseology, Friedrich Schiller University, Jena, Germany.
Bucha
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E
D000818:Animals; D000991:Antithrombins / Q000187:drug effects*; D001777:Blood Coagulation / Q000187:drug effects* / Q000502:physiology; D010450:Endopeptidases / Q000494:pharmacology*; D004792:Enzyme Precursors / Q000494:pharmacology; D005260:Female; D005343:Fibrinolytic Agents / Q000494:pharmacology*; D006629:Hirudins / Q000037:antagonists & inhibitors*; D008297:Male; D009392:Nephrectomy; D011516:Prothrombin / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013917:Thrombin / Q000494:pharmacology; D013918:Thrombin Time
D000991:Antithrombins; D004792:Enzyme Precursors; D005343:Fibrinolytic Agents; D006629:Hirudins; D011516:Prothrombin; D010450:Endopeptidases; D013917:Thrombin; C026812:meizothrombin; C020097:ecarin