title: Neurofibromin-deficient fibroblasts fail to form perineurium in vitro.
abstract: To identify cell type(s) that might contribute to nerve sheath tumors (neurofibromas) in patients with neurofibromatosis type 1, we generated cell cultures containing neurons. Schwann cells and fibroblasts from transgenic mouse embryos in which the type 1 neurofibromatosis gene was disrupted by homologous recombination (Brannan et al. (1994) Genes Development, 8,1019-1029). Normal fascicle formation by perineurial cells failed to occur in the absence of neurofibromin. Fascicles were reduced in number and showed abnormal morphology when normal neurons and Schwann cells were cultured up to 37 days with fibroblasts lacking neurofibromin. Proliferation was increased in a majority of fibroblast cell strains analyzed from embryos lacking neurofibromin. These observations suggest that mutations in the neurofibromatosis type I gene affect fibroblast behavior that might contribute to neurofibroma formation in patients with neurofibromatosis type 1.
authors.affiliation: Department of Cell Biology, University of Cincinnati College of Medicine, Ohio 45267-0521, USA.
authors.forename: T
authors.initials: T
authors.lastname: Rosenbaum
authors.forename: Y L
authors.initials: YL
authors.lastname: Boissy
authors.forename: K
authors.initials: K
authors.lastname: Kombrinck
authors.forename: C I
authors.initials: CI
authors.lastname: Brannan
authors.forename: N A
authors.initials: NA
authors.lastname: Jenkins
authors.forename: N G
authors.initials: NG
authors.lastname: Copeland
authors.forename: N
authors.initials: N
authors.lastname: Ratner
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D002455:Cell Division / Q000502:physiology; D002478:Cells, Cultured; D005347:Fibroblasts / Q000473:pathology*; D011905:Genes, ras; D051379:Mice; D008822:Mice, Transgenic; D008858:Microscopy, Phase-Contrast; D008959:Models, Neurological; D009154:Mutation; D009455:Neurofibroma / Q000196:embryology* / Q000473:pathology; D009456:Neurofibromatosis 1 / Q000196:embryology* / Q000235:genetics / Q000473:pathology; D025542:Neurofibromin 1; D009474:Neurons / Q000473:pathology; D010525:Peripheral Nerves / Q000196:embryology*; D011506:Proteins / Q000502:physiology*; D012583:Schwann Cells / Q000473:pathology
chemical_list: D025542:Neurofibromin 1; D011506:Proteins