Neuroendocrine characteristics of human Leydig cell tumours.
The neuroendocrine nature of a subset of Leydig cells has already been established. The present investigation deals with neuroendocrine characteristics of Leydig tumour cells. A number of neuroendocrine and neuronal markers were demonstrated in Leydig cell tumours of 7 men aged 25-41 years. The following substances were immunocytochemically tested in Leydig tumour cells: the monoamine-synthesizing enzymes tyrosine hydroxylase and aromatic L-amino acid decarboxylase, the indoleamine serotonin, the calcium-binding protein parvalbumin, the microtubule associated protein-2, neurofilament protein 200, synaptophysin, neuron specific enolase, substance P and neuronal nitric oxide synthase (NOS). Compared to the normal interstitial cells beyond the tumours, all neoplastic cells showed a significantly weaker immunoreactivity for nerve cell markers as well as for testosterone and cyclic guanosine monophosphate (cGMP), which is usually accumulated by nitric oxide (NO). This provides evidence for a certain dedifferentiation of Leydig tumour cells. However, these results suggest that tumourous development of Leydig cells does not include loss of neuronal phenotype. Moreover, on the assumption that 'neuronal' Leydig cells exist beside 'non-neuronal' ones in normal testicular tissue, we propose the hypothesis that 'neuronal' Leydig cells can transform to tumour cells.
Middendorff
R
R
Institute of Anatomy, University of Hamburg, Germany.
Davidoff
M S
MS
Mayer
B
B
Holstein
A F
AF
D000328:Adult; D006152:Cyclic GMP / Q000032:analysis; D006801:Humans; D007150:Immunohistochemistry; D007984:Leydig Cell Tumor / Q000737:chemistry*; D008297:Male; D009419:Nerve Tissue Proteins / Q000032:analysis; D009474:Neurons / Q000737:chemistry; D009490:Neurosecretory Systems / Q000737:chemistry*; D019001:Nitric Oxide Synthase / Q000032:analysis; D013736:Testicular Neoplasms / Q000737:chemistry*; D013739:Testosterone / Q000032:analysis
D009419:Nerve Tissue Proteins; D013739:Testosterone; D019001:Nitric Oxide Synthase; D006152:Cyclic GMP