Simultaneous measurement of ethanol and ethyl-d5 alcohol by stable isotope gas chromatography-mass spectrometry.
Efforts to define gender- and ethnic-dependent differences in ethanol first-pass metabolism by gastric mucosa and liver have been limited by a lack of analytical tools that distinguish ethanol concurrently administered by oral and intravenous routes. A stable isotope gas chromatography-mass spectrometry method for simultaneous measurement of ethanol and ethyl-d5 alcohol in serum was developed to meet this need. The assay was linear from 1 to 30 mmol/L. The limit of quantification was 1 mmol/L. Analytical imprecision (CV) was <10%. Analytical recovery was >90%. Specificity was based on retention time and reproducibility of ion ratios. The assay was free from interference by other volatile alcohols. Simultaneous oral administration of ethanol and ethyl-d5 alcohol produced nearly identical pharmacokinetic profiles. Simultaneous oral ingestion of ethanol and intravenous infusion of ethyl-d5 alcohol, adjusted for gastric emptying time, revealed decreased bioavailability of ethanol by the oral route. The method described is sufficient to study the first-pass metabolism of ethanol.
Dean
R A
RA
Department of Pathology, Indiana University School of Medicine, Indianapolis, 46202, USA.
Thomasson
H R
HR
Dumaual
N
N
Amann
D
D
Li
T K
TK
D000284:Administration, Oral; D000328:Adult; D001682:Biological Availability; D000431:Ethanol / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D005746:Gastric Emptying; D005753:Gastric Mucosa / Q000378:metabolism; D006801:Humans; D007262:Infusions, Intravenous; D008099:Liver / Q000378:metabolism; D008297:Male; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013667:Technetium
D000431:Ethanol; D013667:Technetium