The suitability of DNA cytometry for the prediction of the histological diagnosis in women with abnormal cervical smears.
OBJECTIVE
To analyse the suitability of DNA cytometry for predicting the histological diagnosis in women with cervical dyskaryosis.


METHODS
Survey with the use of diagnostic information to revise disease probability.


METHODS
Colposcopy clinic of a university hospital.


METHODS
One hundred and ten women with two mildly or moderately dyskaryotic cervical smears and 98 women with one severely dyskaryotic smear.


METHODS
DNA cytometric analysis using cytocentrifuge preparations of single cell suspensions from a cervical scrape. The main DNA cytometric parameter was N5C (i.e. the absolute number of cells with a DNA content of more than 5C on a given surface with a predefined cell density).


METHODS
The probability of finding CIN II or worse. On arbitrary grounds, a positive test should point to a probability of 85% or higher.


RESULTS
In the patients with cervical neoplasia, the value of N5C increased significantly with an increasing CIN grade (P < 0.001). In the patients with one severely dyskaryotic smear and in those with two mildly or moderately dyskaryotic smears, the prior probability of finding CIN II or worse was 94% and 53%, respectively. Therefore, DNA cytometric analysis might be particularly useful in women with mild or moderate dyskaryosis; further analysis was restricted to this group. All of the women in whom the N5C value was higher than 52 were diagnosed as having CIN II or worse. Only 16 (14.5%) of the 110 women had an N5C value of 52 or higher. When the N5C value was 27, the probability of finding CIN II or worse was estimated to be 85%. Only 28 (25%) patients had an N5C value of 27 or higher.


CONCLUSIONS
DNA cytometry produced significant diagnostic information, as was shown by the relation between N5C and the histological diagnosis. However, the N5C value could not discriminate sufficiently between women with CIN II or worse and CIN I or better. Therefore, the management of individual patients with cytological abnormalities cannot be based on the results of DNA cytometric analysis.
van Leeuwen
A M
AM
Department of Obstetrics and Gynaecology, University Hospital, Groningen, The Netherlands.
Ploem-Zaaijer
J J
JJ
Pieters
W J
WJ
Hollema
H
H
Burger
M P
MP
D000328:Adult; D000368:Aged; D002452:Cell Count; D004247:DNA / Q000032:analysis* / Q000235:genetics; D004273:DNA, Neoplasm / Q000032:analysis / Q000235:genetics; D005189:False Positive Reactions; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008875:Middle Aged; D011003:Ploidies; D002577:Uterine Cervical Diseases / Q000175:diagnosis* / Q000235:genetics; D002583:Uterine Cervical Neoplasms / Q000175:diagnosis / Q000235:genetics; D014626:Vaginal Smears; D002578:Uterine Cervical Dysplasia / Q000175:diagnosis / Q000235:genetics
D004273:DNA, Neoplasm; D004247:DNA