Dose-response effects of recombinant bovine somatotropin (Posilac) on growth performance and body composition of two-year-old rainbow trout (Oncorhynchus mykiss).
Two hundred rainbow trout (Oncorhynchus mykiss, mean weight 301.5 g) were allotted to four treatments with five replicates in a randomized block design to determine the dose-response effects of recombinant bovine somatotropin (rbST; Posilac) on growth performance and carcass composition. Treatments were sham-injected controls (S), 10 micrograms/g BW of rbST (L), 20 micrograms/g BW of rbST (M), and 30 micrograms/g BW of rbST (H). The tanks held 135 L; water flow = 15.1 L/min; temperature = 15 degrees C. The fish were maintained in a 12-h light:dark cycle and hand-fed twice daily. The fish received a single intraperitoneal (i.p.) injection on d 0 and were weighed on d 0, 14, 28, and 56. On d 56 the fish were killed. The whole body (WBW), eviscerated carcass (EC), viscera (VIS), and reproductive organ weights and the increase in average daily body length (ADL) were determined. Recombinant bST reduced (linear, P < .004) feed intake 17.6% from d 0 to 14 and improved ADG 44.8% from d 0 to 14 (linear, P < .001) and 8.1% from d 0 to 56 (linear, P < .022). Treated groups had improved (linear, P < .001) feed efficiencies for d 0 to 28. Treatment with rbST increased final weight (linear, P < .018) and length (linear, P < .001), decreased carcass dry matter (linear, P < .001) and fat (linear, P < .001), content, increased carcass ash (linear, P < .001) and tended to increase carcass protein (linear, P < .054) content. Recombinant bST increased WBW (linear, P < .018) and EC (linear, P < .003) but decreased (linear, P < .015) testes weight. Ovary weights, VIS and overall gonadosomatic index were unaffected (P > .05) by rbST. Recombinant bST was undetectable in serum samples taken on d 56 as determined by radioimmunoassay. Overall, the improved ADG, feed efficiency, body mass, and composition indicate that administration of rbST to rainbow trout may be an efficacious method of modulating growth in fish.
Garber
M J
MJ
Department of Animal and Veterinary Science, University of Idaho, Moscow 83844-2330, USA.
DeYonge
K G
KG
Byatt
J C
JC
Lellis
W A
WA
Honeyfield
D C
DC
Bull
R C
RC
Schelling
G T
GT
Roeder
R A
RA
D000818:Animals; D017756:Aquaculture; D001823:Body Composition / Q000187:drug effects* / Q000502:physiology; D002417:Cattle; D004305:Dose-Response Relationship, Drug; D005260:Female; D013006:Growth Hormone / Q000097:blood / Q000494:pharmacology*; D008297:Male; D017686:Oncorhynchus mykiss / Q000254:growth & development* / Q000502:physiology; D009929:Organ Size; D010053:Ovary / Q000033:anatomy & histology / Q000187:drug effects; D011863:Radioimmunoassay; D011897:Random Allocation; D011994:Recombinant Proteins / Q000097:blood / Q000494:pharmacology; D013737:Testis / Q000033:anatomy & histology / Q000187:drug effects
D011994:Recombinant Proteins; D013006:Growth Hormone