Cell-mediated immunity to pseudorabies virus: cytolytic effector cells with characteristics of lymphokine-activated killer cells lyse virus-infected and glycoprotein gB- and gC-transfected L14 cells.
We examined cytolytic cells that lyse pseudorabies virus (PRV)-infected cells in pigs. In vitro stimulation of peripheral blood mononuclear cells from PRV-immune pigs with live PRV generated cells that lysed PRV-infected immortalized B cells. Several lines of evidence indicated a major contribution of non-major histocompatibility complex (MHC)-restricted cytolytic cells, which displayed characteristics of natural killer (NK) or lymphokine-activated killer cells: cytolysis was non-MHC-restricted, depended on CD2+CD4-CD8bright- (or CD2+CD4-CD8dull+) cells, was strongly augmented by in vitro antigenic stimulation and was not limited to virus-infected cells, i.e. the NK cell-susceptible target cell line K562 was also lysed. Cytolytic cells were also generated by in vitro antigenic stimulation with UV-inactivated PRV. Target cells transfected with and stably expressing PRV gB or gC were lysed to the same degree as PRV-infected target cells.
Kimman
T G
TG
Department of Porcine and Exotic Viral Diseases, Institute for Animal Science and Health, Lelystad, The Netherlands.
DeBruin
T G
TG
Voermans
J J
JJ
Bianchi
A T
AT
D000818:Animals; D002460:Cell Line; D003602:Cytotoxicity, Immunologic; D011558:Herpesvirus 1, Suid / Q000276:immunology*; D007111:Immunity, Cellular; D015979:Killer Cells, Lymphokine-Activated / Q000276:immunology*; D013552:Swine; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014162:Transfection; D014759:Viral Envelope Proteins / Q000235:genetics / Q000502:physiology*
D014759:Viral Envelope Proteins; C042966:pseudorabies virus glycoproteins