The organelles containing porcine 17 beta-estradiol dehydrogenase are peroxisomes.
Porcine 17 beta-estradiol dehydrogenase was recently purified and cloned. It catalyzes the NAD(+)-dependent oxidation of estradiol to estrone 360-fold more efficiently than the reverse reaction with NADPH. Immunogold electron microscopy localizes 17 beta-estradiol dehydrogenase in organelles of 120 to 500 nm with moderate electron-dense matrices bounded by single membranes. Antibodies against the peroxisomal markers catalase and acyl-CoA oxidase recognize the same organelles in double-labeling studies. This is the first report on the participation of peroxisomes in the metabolism of estradiol.
Markus
M
M
Max-Planck-Institut für experimentelle Endokrinologie, Hannover, Germany.
Husen
B
B
Leenders
F
F
Jungblut
P W
PW
Hall
P F
PF
Adamski
J
J
D042962:Acyl-CoA Oxidase; D000818:Animals; D002374:Catalase / Q000032:analysis; D004717:Endometrium / Q000201:enzymology; D004960:Estradiol Dehydrogenases / Q000032:analysis*; D005260:Female; D007672:Kidney Cortex / Q000201:enzymology; D008830:Microbodies / Q000201:enzymology*; D010088:Oxidoreductases / Q000032:analysis; D013552:Swine
D010088:Oxidoreductases; D004960:Estradiol Dehydrogenases; D002374:Catalase; D042962:Acyl-CoA Oxidase