[Cytochrome b-558 from blood serum and erythrocyte membranes. Isolation, purification, and brief characteristics].
Four types of water-soluble cytochromes b-558 (cyt. b-558) have been isolated and purified from blood serum (cyt. b-558 (I), cyt. b-558 (II)) and erythrocyte membranes (cyt. b-558 (III) and cyt. b-558 (IV)). The cytochromes differ in their acid-basic properties, molecular masses (110, 95, 125 and 80 kDa for cyt. b-558 (I), cyt. b-558 (II), b-558 (III) and cyt. b-558 (IV), respectively) as well as by the composition (cyt. b-558 (III) and cyt. b-558 (IV) are lipoproteins) and absorption maxima at the wavelengths of 340-360 nm. The cytochromes have similar absorption maxima at the wavelengths 600-400 nm (560, 530 and 412 nm in the oxidized state) and 558, 525 and 420 nm in the reduced state) which makes them identical to cytochromes b-558 obtained from blood constituents membranes.
Simonian
M A
MA
Babaian
M A
MA
Simonian
G M
GM
D002852:Chromatography, Ion Exchange; D003573:Cytochrome b Group / Q000097:blood* / Q000737:chemistry / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D004910:Erythrocyte Membrane / Q000201:enzymology*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D019255:NADPH Oxidases; D013057:Spectrum Analysis
D003573:Cytochrome b Group; C036888:cytochrome b558; D019255:NADPH Oxidases