Detection of chromosome 20q deletions in bone marrow metaphases but not peripheral blood granulocytes in patients with myeloproliferative disorders or myelodysplastic syndromes.
Myeloproliferative disorders and myelodysplastic syndromes arise in multipotent progenitors and may be associated with chromosomal deletions that can be detected in peripheral blood granulocytes. We present here seven patients with myeloproliferative disorders or myelodysplastic syndromes in whom a deletion of the long arm of chromosome 20 was detectable by G-banding and/or fluorescence in situ hybridization in most or all bone marrow metaphases. However, in each case, microsatellite polymerase chain reaction (PCR) using 15 primer pairs spanning the common deleted region on 20q showed that the deletion was absent from most peripheral blood granulocytes. The human androgen receptor clonality assay was used to show that the vast majority of peripheral blood granulocytes were clonal in all four female patients. This represents the first demonstration that the 20q deletion can arise as a second event in patients with pre-existing clonal granulopoiesis. Microsatellite PCR analysis of whole bone marrow from two patients was consistent with cytogenetic studies, a result that suggests that cytogenetic analysis was not merely selecting for a minor subclone of cells carrying the deletion. Furthermore, in one patient, the deletion was present in both erythroid and granulocyte/monocyte colonies. This implies that the absence of the deletion in most peripheral blood granulocytes did not reflect lineage restriction of the progenitors carrying the deletion but may instead result from other selective influences such as preferential retention/destruction within the bone marrow of granulocytes carrying the deletion.
Asimakopoulos
F A
FA
University of Cambridge Department of Haematology, MRC Centre, UK.
Holloway
T L
TL
Nacheva
E P
EP
Scott
M A
MA
Fenaux
P
P
Green
A R
AR
D000368:Aged; D001853:Bone Marrow / Q000473:pathology; D002869:Chromosome Aberrations / Q000175:diagnosis* / Q000473:pathology; D002872:Chromosome Deletion; D025063:Chromosome Disorders; D002890:Chromosomes, Human, Pair 20; D005260:Female; D005819:Genetic Markers; D006098:Granulocytes / Q000473:pathology; D006720:Homozygote; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008875:Middle Aged; D009190:Myelodysplastic Syndromes / Q000235:genetics / Q000473:pathology*; D009196:Myeloproliferative Disorders / Q000235:genetics / Q000473:pathology*; D011110:Polymorphism, Genetic
D005819:Genetic Markers