Differential labelings suggest two specific phospholipid subclass hydrolysis promoted by PDGF-BB in vascular smooth muscle cells.
We have used differential phospholipid subclass labelings performed with [3H]lyso PAF and [3H] myristic acid into vascular smooth muscle cells (vSMC) to characterize the subclasses of phospholipid substrates upon different stimulation times with platelet-derived growth factor (PDGF-BB). In cells labeled with [3H]lyso PAF, PDGF-BB induced a sustained hydrolysis of alkyl-PE. In contrast, in [3H]myristic acid-labeled cells, PDGF-BB promoted a rapid and transitory hydrolysis of diacyl-PC. This hydrolysis was concomitant with an synthesis of diglyceride (DG) and phosphatidic acid (PA). Thus, both diacyl-PC and alkyl-PE appear to be major targets in PDGF-BB stimulation of SMC. These results suggest that agonists could induce the hydrolysis of precise phospholipid subclasses leading to a new specificity into the signal transduction cascade.
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Institut National de Santé et de la Recherche Médicale, Hôpital Purpan, Toulouse, France.
Maury
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Cariven
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D000818:Animals; D001013:Aorta, Thoracic / Q000378:metabolism*; D000077214:Becaplermin; D002478:Cells, Cultured; D006868:Hydrolysis; D007700:Kinetics; D019814:Myristic Acid; D009227:Myristic Acids / Q000378:metabolism; D010743:Phospholipids / Q000145:classification / Q000302:isolation & purification / Q000378:metabolism*; D010972:Platelet Activating Factor / Q000031:analogs & derivatives / Q000378:metabolism; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology*; D020574:Proto-Oncogene Proteins c-sis; D011865:Radioisotope Dilution Technique; D011994:Recombinant Proteins / Q000494:pharmacology; D013552:Swine; D013997:Time Factors; D014316:Tritium; D017540:Tunica Media / Q000187:drug effects / Q000378:metabolism*
D009227:Myristic Acids; C029271:O-deacetyl platelet activating factor; D010743:Phospholipids; D010972:Platelet Activating Factor; D010982:Platelet-Derived Growth Factor; D020574:Proto-Oncogene Proteins c-sis; D011994:Recombinant Proteins; D019814:Myristic Acid; D014316:Tritium; D000077214:Becaplermin