title: Light-induced acceleration of photoreceptor degeneration in transgenic mice expressing mutant rhodopsin.
abstract: OBJECTIVE
  Mutations at various loci on the rhodopsin gene have been shown to cause autosomal dominant retinitis pigmentosa (ADRP). One of the most common is a point mutation (P23H) near the N-terminus of the protein. The authors have studied the effects of light deprivation on the rate of degeneration in pigmented transgenic mice expressing the P23H mutation as well as two additional mutations near the N-terminus of opsin (V20G, P27L).
  
  
  METHODS
  Transgenic and normal littermates were reared in darkness or in cyclic light (approximately 7 foot-candle) for periods of 2, 4, or 6 months. Retinal structure and function were evaluated by electroretinography, retinal densitometry, light microscopy, and TUNEL labeling.
  
  
  RESULTS
  Retinas of normal animals, whether reared in darkness or in cyclic light, had no structural or functional abnormalities. The rate of photoreceptor degeneration in dark-reared transgenic mice was significantly slower than in transgenic mice raised under cyclic light conditions. Differences between the two groups of animals were evident in the retinal histology, the electroretinographically determined sensitivity to photic stimulation, and the rhodopsin levels in the retina. TUNEL labeling of retinal wholemounts showed that cyclic light-reared animals had a threefold higher incidence of photoreceptor cell death than their dark-reared counterparts; the density of apoptotic cells was greatest in the inferior retina, the region most severely affected in patients with the P23H mutation. In comparison, photoreceptor cell death was more uniformly distributed across the retina in dark-reared transgenic mice.
  
  
  CONCLUSIONS
  These findings suggest that light activation of rhodopsin contributes to the severity of the degenerative disease resulting from the P23H opsin mutation, and they raise the possibility that minimizing exposure to light may help to prolong useful vision of patients with this form of retinitis pigmentosa.
authors.affiliation: Department of Ophthalmology and Visual Sciences, University of Illinois at Chicago Eye Center, IL 60612, USA.
authors.forename: M L
authors.initials: ML
authors.lastname: Naash
authors.forename: N S
authors.initials: NS
authors.lastname: Peachey
authors.forename: Z Y
authors.initials: ZY
authors.lastname: Li
authors.forename: C C
authors.initials: CC
authors.lastname: Gryczan
authors.forename: Y
authors.initials: Y
authors.lastname: Goto
authors.forename: J
authors.initials: J
authors.lastname: Blanks
authors.forename: A H
authors.initials: AH
authors.lastname: Milam
authors.forename: H
authors.initials: H
authors.lastname: Ripps
mesh_terms: D000818:Animals; D017209:Apoptosis; D001483:Base Sequence; D016923:Cell Death; D017931:DNA Primers / Q000737:chemistry; D003623:Dark Adaptation; D004596:Electroretinography; D005260:Female; D015870:Gene Expression; D008027:Light / Q000009:adverse effects*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D010786:Photoreceptor Cells / Q000473:pathology* / Q000503:physiopathology; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D012162:Retinal Degeneration / Q000209:etiology* / Q000235:genetics / Q000473:pathology / Q000503:physiopathology; D012243:Rhodopsin / Q000235:genetics* / Q000502:physiology; D012683:Sensory Deprivation
chemical_list: D017931:DNA Primers; D012243:Rhodopsin