[15-19-mer synthetic oligoribonucleotide as a model of the translation initiation segment of the phage fr replicase gene].
Automatic solid-phase synthesis of 15-19-meric oligoribonucleotides was carried out using 5'-O-dimethoxytrityl-2'-O-(2-tetrahydropyranyl)- [or 2'-O-(2-tetrahydrofuranyl)-] N-acylribonucleoside-3'-O-(methyl-N, N-diisopropyl)phosphoramidite synthons and 1-H-tetrazole as an activator. Comparative analysis of the template activity of the oligoribonucleotides synthesized, which are models of the translation initiation region of the replicase gene of MS2 and fr phages, showed that the minimal active fragment of RNA is a 16-mer containing the initiation AUG codon of the gene, a short spacer, a Shine-Dalgarno domain, and the 5'-terminal AUGA sequence with a functionally important termination AUG codon.
Kumpin'sh
V Kh
VKh
Litsis
N G
NG
Renkhof
R F
RF
Stankevich
E I
EI
Berzin'
V M
VM
D001435:Bacteriophages / Q000201:enzymology*; D001483:Base Sequence; D018388:Codon, Terminator; D008957:Models, Genetic; D008969:Molecular Sequence Data; D009843:Oligoribonucleotides / Q000138:chemical synthesis* / Q000378:metabolism; D010442:Peptide Chain Initiation, Translational; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics*
D018388:Codon, Terminator; D009843:Oligoribonucleotides; D012324:RNA-Dependent RNA Polymerase