Oligosaccharide sequencing based on exo- and endoglycosidase digestion and liquid chromatographic analysis of the products.
Exo- and endoglycosidases are used to sequence oligosaccharides and give valuable information on the monosaccharide sequence, together with the anomericity, the stereochemistry, and in some cases, the substitution pattern of the monosaccharides. Both sequential and parallel methods of oligosaccharide sequencing are discussed.
Prime
S
S
Oxford GlycoSystems Ltd., Hitching Court, Abingdon, Oxon, UK.
Dearnley
J
J
Ventom
A M
AM
Parekh
R B
RB
Edge
C J
CJ
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002853:Chromatography, Liquid; D006026:Glycoside Hydrolases / Q000378:metabolism*; D006868:Hydrolysis; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification; D011134:Polysaccharides / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification
D009844:Oligosaccharides; D011134:Polysaccharides; D006026:Glycoside Hydrolases