[Characterization of bronchoalveolar space cells in pulmonary sarcoidosis].
The aim of this study was to evaluate the number, composition and the activity of bronchoalveolar space cells as well as peripheral blood lymphocytes in patients with a different stages of pulmonary sarcoidosis. A particular attention was paid on relations between composition of the cells, the stage of sarcoidosis and the influence of cigarette smoking or air pollution. The studies were carried out on 110 patients with sarcoidosis who were not treated using steroids and without additional respiratory tract infections. As a control, 12 non smoking persons were used with no pathological changes in the lungs. The number and percentage of the cells from bronchoalveolar space were determined by routine cytological method. The assessment of monocyte macrophages was performed by computer cytomorphometry (monoclonal antibodies CD14, CD68) and immunoenzymatic method BS-A. A flow-fluorocytometer and monoclonal antibodies CD3, CD4, CD8, CD19 were applied to define lymphocyte subsets. We conclude that cytology of bronchoalveolar fluid is characteristic for early stages of sarcoidosis, but a typical view appears in more advanced stage of the disease. The additional exogenous factors like a cigarette smoking may change the activity and the composition of cell subsets in bronchoalveolar space in the patients with lung sarcoidosis. For this reason, an individual interpretation of data obtained is needed in each case.
Czarnobilska
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Zakład Patofizjologii Klinicznej Miedzywydziałowej Katedry Patofizjologii Collegium Medicum UJ w Krakowie.
D000328:Adult; D015703:Antigens, CD / Q000032:analysis; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology*; D005260:Female; D006801:Humans; D007958:Leukocyte Count; D016131:Lymphocyte Subsets; D008297:Male; D008875:Middle Aged; D017565:Sarcoidosis, Pulmonary / Q000473:pathology*; D012907:Smoking / Q000009:adverse effects / Q000473:pathology
D015703:Antigens, CD