A stable Escherichia coli-mycobacteria shuttle vector 'pSO246' in Mycobacterium bovis BCG.
The most widely used plasmid vector system in mycobacteria is based on pAL5000 from Mycobacterium fortuitum. The derivatives of the pAL5000-based shuttle vectors between Escherichia coli and mycobacteria, which we have utilized to secrete recombinant antigens, were generated. The stability of the vectors was assessed in Mycobacterium bovis BCG (BCG). The plasmid vector pSO246 was stable in BCG for at least 50 generations.
Matsumoto
S
S
Nagasaki University, School of Dentistry, Japan.
Tamaki
M
M
Yukitake
H
H
Matsuo
T
T
Naito
M
M
Teraoka
H
H
Yamada
T
T
D001426:Bacterial Proteins / Q000032:analysis; D004926:Escherichia coli / Q000235:genetics*; D005822:Genetic Vectors / Q000235:genetics*; D007613:Kanamycin Resistance; D008970:Molecular Weight; D009163:Mycobacterium bovis / Q000235:genetics*; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000032:analysis
D001426:Bacterial Proteins; D011994:Recombinant Proteins