title: Comparison of assays for Cryptosporidium parvum oocysts viability after chemical disinfection.
abstract: In vitro excystation, vital dyes (4', 6-diamidino-2-phenylindole (DAPI) and propidium iodide (PI), and infectivity in neonatal CD-1 mice were used to assess the viability of Cryptosporidium parvum oocysts after chemical disinfection. In vitro excystation and DAPI/PI staining provided similar estimates of viability in bench-scale experiments, but both of these methods significantly overestimated the viability when compared with infectivity (Pr < or = 0.01). Infectivity was the most reliable measure of the viability of C. parvum oocysts following chemical disinfection.
authors.affiliation: Department of Civil Engineering, University of Alberta, Edmonton, Canada.
authors.forename: E K
authors.initials: EK
authors.lastname: Black
authors.forename: G R
authors.initials: GR
authors.lastname: Finch
authors.forename: R
authors.initials: R
authors.lastname: Taghi-Kilani
authors.forename: M
authors.initials: M
authors.lastname: Belosevic
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D016785:Cryptosporidium parvum / Q000302:isolation & purification / Q000472:pathogenicity / Q000502:physiology*; D004203:Disinfection; D005456:Fluorescent Dyes; D007211:Indoles; D051379:Mice; D011419:Propidium
chemical_list: D005456:Fluorescent Dyes; D007211:Indoles; D011419:Propidium; C007293:DAPI