Genotoxicity of vanadium pentoxide evaluate by the single cell gel electrophoresis assay in human lymphocytes.
Vanadium compounds are extensively used in modern industry and occupational exposure to high doses of Vanadium is quite common. In this study, the genotoxicity of vanadium pentoxide (V2O5) was evaluated directly in whole blood leukocytes and in human lymphocyte cultures using the single-cell gel electrophoresis assay (Comet Assay) to detect DNA damage expressed as DNA strand breaks and alkali labile sites. This chemical produces a clear dose-response in DNA migration in whole blood leukocytes and a significative positive effect only with the highest tested concentration in human lymphocyte cultures. After different recovery times the level of DNA damage returned to the control values. These results indicate that V2O5 is capable to induce DNA single-strand breaks and/or alkali-labile damage.
Rojas
E
E
Instituto de Investigaciones Biomedicas, U.N.A.M., Mexico.
Valverde
M
M
Herrera
L A
LA
Altamirano-Lozano
M
M
Ostrosky-Wegman
P
P
D000328:Adult; D004247:DNA / Q000187:drug effects*; D004249:DNA Damage; D004260:DNA Repair; D004586:Electrophoresis; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects; D008297:Male; D017968:Vanadium Compounds / Q000633:toxicity*
D017968:Vanadium Compounds; D004247:DNA; C066075:vanadium pentoxide