Direct measurement of oscillatory generation of superoxide anions by single phagocytes.
Phagocytic cells such as neutrophils generate superoxide anions (O2-) within phagocytic vacuoles for killing and digesting microorganisms. Here we report the simultaneous observation of morphological changes and O2- generation in single phagocytic cells during phagocytosis. Point stimulation of a cell by contact with an opsonized microelectrode at the cell surface induced significant deformation to engulf the electrode, and also induced the O2- generation which was measured by the electrode. Periodic fluctuations in the magnitude of the O2- generation were observed in the time course. These oscillations may be caused by metabolic regulation of the formation of NADPH, which is the substrate for the O2- generation
Isogai
Y
Y
Laboratory of Biophysical Chemistry, Institute of Physical and Chemical Research (RIKEN), Saitama, Japan.
Tsuyama
T
T
Osada
H
H
Iizuku
T
T
Tanaka
K
K
D000838:Anions; D006801:Humans; D007074:Immunoglobulin G; D007700:Kinetics; D015473:Leukemia, Promyelocytic, Acute; D008839:Microelectrodes; D009249:NADP / Q000378:metabolism; D009895:Opsonin Proteins; D010586:Phagocytes / Q000378:metabolism*; D010587:Phagocytosis; D013481:Superoxides / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate; D014212:Tretinoin / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D000838:Anions; D007074:Immunoglobulin G; D009895:Opsonin Proteins; D013481:Superoxides; D009249:NADP; D014212:Tretinoin; D013755:Tetradecanoylphorbol Acetate