title: Pathological considerations in cryopreserved allograft heart valves.
abstract: Since structure-function correlations have not been determined for cryopreserved allograft heart valves, we studied 20 explanted valves in place several hours to nine years, as either orthotopic aortic valves/root replacements or right ventricle to pulmonary artery conduits. They were explanted primarily due to growth-related conduit or valve stenosis, valve regurgitation, or infection. Controls included seven unused cryopreserved allograft valves and 16 aortic valves removed from transplanted allograft hearts obtained at either autopsy (n = 15) or retransplantation (n = 1), two days to four years postoperatively, following myocardial rejection (n = 4), graft coronary arteriosclerosis (n = 4), and other (n = 8). Analysis included gross inspection, radiography, light microscopy, electron microscopy, and immunohistochemical studies (to allow identification/localization of endothelial cells, mononuclear inflammatory cells, and T and B lymphocyte subsets). Cryopreserved allograft valves implanted more than one day had progressively severe loss of normal structure and were devoid of surface endothelium and stainable deep connective tissue cells. Inflammatory cellularity varied from none (most valves) to prominent (primarily T lymphocytes in one valve). Transmission electron microscopy of three long term valvular allografts revealed no viable cells, remarkable preservation of the collagenous valve matrix and focal cell-oriented calcification. In contrast, aortic valves from transplanted hearts showed remarkable structural preservation, including layered architecture, endothelium and deep connective tissue cells; inflammatory infiltrates were generally sparse, even in cases with fatal myocardial rejection. We conclude that cryopreserved allograft valves are morphologically non-viable valves, whose structural basis for function seems primarily related to the largely preserved collagen.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Pathology, Brigham and Women's Hospital, Boston, MA 02115, USA.
authors.forename: F J
authors.initials: FJ
authors.lastname: Schoen
authors.forename: R N
authors.initials: RN
authors.lastname: Mitchell
authors.forename: R A
authors.initials: RA
authors.lastname: Jonas
mesh_terms: D001021:Aortic Valve / Q000000981:diagnostic imaging / Q000473:pathology* / Q000637:transplantation*; D001022:Aortic Valve Insufficiency / Q000601:surgery; D001024:Aortic Valve Stenosis / Q000601:surgery; D016175:B-Lymphocyte Subsets / Q000473:pathology; D002114:Calcinosis / Q000473:pathology; D002470:Cell Survival; D003094:Collagen; D003238:Connective Tissue / Q000473:pathology; D003324:Coronary Artery Disease / Q000601:surgery; D015925:Cryopreservation; D004549:Elastin; D004697:Endocarditis, Bacterial / Q000601:surgery; D004727:Endothelium / Q000473:pathology; D005500:Follow-Up Studies; D006084:Graft Rejection / Q000601:surgery; D006801:Humans; D007150:Immunohistochemistry; D007963:Leukocytes, Mononuclear / Q000473:pathology; D008854:Microscopy, Electron; D011664:Pulmonary Valve / Q000000981:diagnostic imaging / Q000473:pathology* / Q000637:transplantation*; D011665:Pulmonary Valve Insufficiency / Q000601:surgery; D011666:Pulmonary Valve Stenosis / Q000601:surgery; D011859:Radiography; D016176:T-Lymphocyte Subsets / Q000473:pathology; D014021:Tissue Preservation; D014184:Transplantation, Homologous
chemical_list: D003094:Collagen; D004549:Elastin