title: Thyrotropin-releasing hormone-induced subcellular redistribution and down-regulation of G11alpha: analysis of agonist regulation of coexpressed G11alpha species variants.
abstract: Human embryonic kidney 293 cells that had been transfected to express the long isoform of the rat thyrotropin-releasing hormone (TRH) receptor (clone E2) were further transfected with a cDNA encoding the murine version of G11alpha. A clone was isolated (clone E2M11) that stably expressed murine as well as the endogenous human G11alpha. Subcellular fractionation demonstrated identical cellular distribution of the two species variants of this G protein. Sustained exposure of clone E2M11 cells to TRH resulted in substantial cellular redistribution and reduction in total cellular levels of G11alpha immunoreactivity. Fractions of both the exogenously introduced murine and endogenously expressed human isoforms of G11alpha were transferred from plasma membranes to low density membranes (detected as a shift from middle to low density regions on sucrose density gradients) and cytosol fractions. The plasma membrane redistribution to low density membrane was accompanied by a parallel redistribution of G protein beta subunits; however, there was no increase in beta subunits in the cytosol. The total cellular amount of G11alpha subunits was decreased to 21% and 59% for human and murine isoforms, respectively, and beta subunits were decreased to 68% after sustained treatment with TRH compared with controls (100%). Such data are consistent with the notion that the agonist-occupied long isoform of the rat TRH receptor may be able to partially differentiate between the endogenous (human) and exogenous (murine) G11alpha. This was not a reflection that the murine G protein was expressed but incorrectly folded as both species variants of G11alpha were solubilized equally from E2M11 membranes by sodium cholate. Using this system, we demonstrate both agonist-induced subcellular redistribution and down-regulation of G11alpha and beta subunit proteins in response to activation of a phospholipase C coupled receptor.
authors.affiliation: Institute of Biomedical and Life Sciences, University of Glasgow, Scotland, UK.
authors.forename: P
authors.initials: P
authors.lastname: Svoboda
authors.forename: G D
authors.initials: GD
authors.lastname: Kim
authors.forename: M A
authors.initials: MA
authors.lastname: Grassie
authors.forename: K A
authors.initials: KA
authors.lastname: Eidne
authors.forename: G
authors.initials: G
authors.lastname: Milligan
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D015536:Down-Regulation; D019204:GTP-Binding Proteins / Q000032:analysis*; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D011817:Rabbits; D051381:Rats; D018025:Receptors, Thyrotropin-Releasing Hormone / Q000819:agonists*; D013973:Thyrotropin-Releasing Hormone / Q000494:pharmacology*
chemical_list: D018025:Receptors, Thyrotropin-Releasing Hormone; D013973:Thyrotropin-Releasing Hormone; D019204:GTP-Binding Proteins