odr-10 encodes a seven transmembrane domain olfactory receptor required for responses to the odorant diacetyl.
Olfactory signaling is initiated by interactions between odorants and olfactory receptors. We show that the C. elegans odr-10 gene is likely to encode a receptor for the odorant diacetyl. odr-10 mutants have a specific defect in chemotaxis to diacetyl, one of several odorants detected by the AWA olfactory neurons. odr-10 encodes a predicted seven transmembrane domain receptor; a green fluorescent protein-tagged Odr-10 protein is localized to the AWA sensory cilia. odr-10 expression is regulated by odr-7, a transcription factor implicated in AWA sensory specification. Expression of odr-10 from a heterologous promoter directs behavioral responses to diacetyl, but not to another odorant detected by the AWA neurons. These results provide functional evidence for a specific interaction between an olfactory receptor protein and its odorant ligand.
Sengupta
P
P
Howard Hughes Medical Institute, Programs in Developmental Biology, Neuroscience, and Genetics, University of California, San Francisco, 94143-0452, USA.
Chou
J H
JH
Bargmann
C I
CI
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017173:Caenorhabditis elegans / Q000502:physiology*; D002633:Chemotaxis / Q000187:drug effects / Q000235:genetics; D003931:Diacetyl / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000502:physiology; D017238:Genes, Helminth / Q000502:physiology; D015801:Helminth Proteins / Q000235:genetics / Q000502:physiology / Q000648:ultrastructure*; D008297:Male; D008969:Molecular Sequence Data; D009154:Mutation; D018034:Olfactory Receptor Neurons / Q000502:physiology / Q000648:ultrastructure; D011719:Pyrazines / Q000494:pharmacology; D018035:Receptors, Odorant / Q000235:genetics* / Q000378:metabolism / Q000648:ultrastructure; D012680:Sensitivity and Specificity; D015398:Signal Transduction / Q000502:physiology
D015801:Helminth Proteins; D011719:Pyrazines; D018035:Receptors, Odorant; D003931:Diacetyl