Effect of high-protein feeding on insulin secretion in mice injected with splenocytes from diabetic mice.
We report here our study of the in vitro glucose-induced insulin secretion from mice fed with a high-protein diet and injected with mononuclear splenocytes from streptozotocin-diabetic syngeneic donors. Results show that transfer of "diabetic" splenocytes caused significant diminutions in first phase of stimulated-insulin secretion. Nevertheless, insulin secretory levels attained in recipient mice fed with the high-protein diet were significantly higher than in mice fed with control diets. We also evaluated the effect of high-protein feeding on the capacity of "diabetic" splenocytes to transfer an impaired insulin secretion in recipient mice. For this purpose, splenocytes donor mice were fed with high-protein or control diets and injected with multiple low doses of streptozotocin. "Diabetic" splenocytes from high-protein, or control diets fed donors caused in recipient mice similar impairments in glucose-stimulated insulin secretion. Taken together, these results seem to support the hypothesis of a protective effect of a high-protein diet on the beta-cell function.
Karabatas
L M
LM
Laboratorio de Diabetes Experimental, Centro de Investigaciones Endocrinologicas (CEDIE), Hospital de Ninos "Dr. R. Gutierrez", Buenos Aires, Argentina.
Lombardo
Y
Y
Basabe
J C
JC
D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000276:immunology*; D004044:Dietary Proteins / Q000008:administration & dosage*; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D013311:Streptozocin
D004044:Dietary Proteins; D007328:Insulin; D013311:Streptozocin