Structure of the phosphopeptidomannans from flocculent and non-flocculent yeast Kluyveromyces lactis.
After extraction from whole cells, and purification by gel filtration, the chemical composition and molecular mass estimation of the cell-wall phosphopeptidomannan (PPM) showed no significant difference respectively between flocculent, weakly, very weakly and non-flocculent Kluyveromyces lactis yeast strains. However, when PPMs were tested as ligands of a lectin, extracted from the flocculent strain, the PPM isolated from the flocculent and weakly flocculent strain were recognized to a higher degree than those isolated from the non and very weakly flocculent strains. Acetolysis of PPM extracted from the four strains produced five oligosaccharide fractions corresponding to mono-, di-, tri-, penta-and hexa-saccharides. The flocculent strain was characterised by a high content of di-and penta-saccharides. The 1H NMR analysis of the oligosaccharides demonstrated that the flocculent strain contained equivalent levels of the two mannobioses: Man(alpha 1-->2)Man and Man(alpha 1-->3)Man and of the two mannotrioses Man(alpha 1-->2)Man(alpha 1-->2)Man and Man(alpha 1-->3)Man(alpha 1-->2)Man. In contrast, the non-flocculent and the very weakly flocculent strains contained a single type of mannobiose Man(alpha 1-->2)Man and one type of mannotriose Man(alpha 1-->2)Man(alpha 1-->2)Man.
Bilang
M
M
Université Henri Poincaré, Faculté des Sciences Pharmaceutiques et Biologiques, Laboratoire de Biochimie Microbienne, Nancy, France.
Attioui
F
F
Loppinet
V
V
Michalski
J C
JC
Bonaly
R
R
D000117:Acetylglucosamine / Q000032:analysis; D002240:Carbohydrate Sequence; D002473:Cell Wall / Q000737:chemistry; D002850:Chromatography, Gel; D005428:Flocculation Tests; D007716:Kluyveromyces / Q000737:chemistry* / Q000502:physiology; D037102:Lectins / Q000302:isolation & purification / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008351:Mannans / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification; D008358:Mannose / Q000032:analysis; D008969:Molecular Sequence Data; D009005:Monosaccharides / Q000737:chemistry; D009844:Oligosaccharides / Q000737:chemistry; D010641:Phenotype; D010748:Phosphopeptides / Q000737:chemistry* / Q000302:isolation & purification; D014312:Trisaccharides / Q000032:analysis / Q000737:chemistry
D037102:Lectins; D008351:Mannans; D009005:Monosaccharides; D009844:Oligosaccharides; D010748:Phosphopeptides; D014312:Trisaccharides; C072108:phosphopeptidomannan; C014647:mannobiose; C023872:mannotriose; D008358:Mannose; D000117:Acetylglucosamine