Human immunoglobulins produced in hu-PBL-SCID mice are polyclonal early after xenotransplantation.
Xenotransplantation of human peripheral blood leukocytes (hu-PBL) to severe combined immune-deficient (SCID, hu-PBL-SCID) mice leads to human immunoglobulin (Ig) production in the animals. Here we used a sensitive high-resolution two-dimensional electrophoresis (2D-PAGE) technique to separate, and to analyze, Ig gamma and light chains in sera from SCID mice given hu-PBL for less than 35 days. Human Igs in the model appeared polyclonal until 3 weeks after cell transfer. After this period, oligoclonal Igs progressively replaced this polyclonal background. The 2D-PAGE pattern evolution of human Igs was similar when hu-PBL originated from donors with or without previous contact with the Epstein-Barr virus. The broad diversity of human Igs, and therefore the large expressed B cell repertoire, in hu-PBL-SCID mice during a period that allows manipulation (such as immunization) of the human graft has evident implication for the use of this model in the generation of specific antibodies of desired specificity.
Tissot
J D
JD
Fondation Centre de transfusion sanguine CRS, Lausanne, Switzerland.
Schneider
P
P
Schapira
M
M
Duchosal
M A
MA
D000818:Animals; D000917:Antibody Formation; D001798:Blood Proteins / Q000737:chemistry; D002999:Clone Cells; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D007074:Immunoglobulin G / Q000737:chemistry; D007147:Immunoglobulin Light Chains / Q000737:chemistry; D017710:Lymphocyte Transfusion; D051379:Mice; D016513:Mice, SCID; D013997:Time Factors; D014183:Transplantation, Heterologous
D001798:Blood Proteins; D007074:Immunoglobulin G; D007147:Immunoglobulin Light Chains