title: Requirement of a CCGAC cis-acting element for cold induction of the BN115 gene from winter Brassica napus.
abstract: Mutation of the core pentamer, CCGAC, of two putative low temperature responsive elements (LTREs) in the 5'-proximal region for the winter Brassica napus cold-induced gene BN115 was carried out. Analyses of transient expression of the resultant mutated BN115 promoter-GUS fusions revealed the loss of low-temperature regulation by the promoter. This indicates that the CCGAC sequence is critical to the low-temperature response in the BN115 gene. In contrast, mutation of two G-boxes, CACGTG, staggered between the LTREs in the same region of the promoter did not alter cold-inducible gene expression. Replacement of a possible enhancer region of the BN115 promoter with the enhancer from the CaMV 35S promoter resulted in a several-fold increase in low temperature-induced GUS activity.
authors.affiliation: Plant Research Centre, Agriculture Canada, Central Experimental Farm, Ottawa, Ontario.
authors.forename: C
authors.initials: C
authors.lastname: Jiang
authors.forename: B
authors.initials: B
authors.lastname: Iu
authors.forename: J
authors.initials: J
authors.lastname: Singh
mesh_terms: D001483:Base Sequence; D001937:Brassica / Q000235:genetics*; D003001:Cloning, Molecular; D003080:Cold Temperature; D018744:DNA, Plant; D004742:Enhancer Elements, Genetic; D018506:Gene Expression Regulation, Plant; D008969:Molecular Sequence Data; D016296:Mutagenesis; D011401:Promoter Regions, Genetic; D012045:Regulatory Sequences, Nucleic Acid
chemical_list: D018744:DNA, Plant