Oral immunization with a recombinant cysteine-rich section of the Entamoeba histolytica galactose-inhibitable lectin elicits an intestinal secretory immunoglobulin A response that has in vitro adherence inhibition activity.
The LC3-encoded 52-kDa recombinant protein includes amino acids 758 to 1134 of the 170-kDa subunit of the galactose-inhibitable lectin. Oral immunization of BALB/c mice with the LC3-encoded protein and cholera holotoxin induced an intestinal secretory immunoglobulin A (IgA) response (P < 0.01 compared with the control). There was a negative correlation (P = 0.001) between intestinal anti-LC3 IgA and serum IgA and IgG antibody responses. Intestinal secretions from immunized mice completely inhibited the galactose-specific adherence of axenic trophozoites ot Chinese hamster ovary cells (P < 0.01).
Beving
D E
DE
Department of Medicine, Case Western Reserve University, Cleveland, Ohio, USA.
Soong
C J
CJ
Ravdin
J I
JI
D000268:Adhesiveness; D000284:Administration, Oral; D000818:Animals; D000913:Antibodies, Protozoan / Q000096:biosynthesis*; D016466:CHO Cells; D006224:Cricetinae; D003545:Cysteine; D004748:Entamoeba histolytica / Q000276:immunology*; D005260:Female; D005690:Galactose / Q000494:pharmacology*; D007114:Immunization; D007071:Immunoglobulin A, Secretory / Q000096:biosynthesis*; D007413:Intestinal Mucosa / Q000276:immunology*; D037102:Lectins / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D015800:Protozoan Proteins / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000276:immunology*
D000913:Antibodies, Protozoan; D007071:Immunoglobulin A, Secretory; D037102:Lectins; D015800:Protozoan Proteins; D014614:Vaccines, Synthetic; D003545:Cysteine; D005690:Galactose