Mapping of the red clover necrotic mosaic virus subgenomic RNA.
The red clover necrotic mosaic dianthovirus capsid protein is expressed in vivo from a subgenomic RNA (sgRNA) identical to the 3'-terminal 1.5 kb of RNA-1. The 5' leader sequence of the capsid protein sgRNA is 62 nucleotides, contains a 14-nucleotide putative promoter sequence homologous to the RNA-1 5' terminus, and exhibits a high level of similarity with the tobacco mosaic virus 5' leader translational enhancer element omega. Analysis of the RNA-1 secondary structure, in the region adjacent to the position where the 5' end of the sgRNA was mapped, predicts a stable stem-loop which includes the putative sgRNA promoter element. It is suggested that this structure is important for recognition of the sgRNA transcriptional initiation from the full-length negative-sense RNA-1.
Zavriev
S K
SK
Institute of Agricultural Biotechnology, Moscow, Russia.
Hickey
C M
CM
Lommel
S A
SA
D001483:Base Sequence; D002213:Capsid / Q000235:genetics; D002874:Chromosome Mapping; D015967:Gene Expression Regulation, Viral; D016679:Genome, Viral; D008969:Molecular Sequence Data; D009029:Mosaic Viruses / Q000235:genetics*; D009154:Mutation; D009690:Nucleic Acid Conformation; D012330:RNA, Double-Stranded; D012367:RNA, Viral / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*
D012330:RNA, Double-Stranded; D012367:RNA, Viral