Prolactin receptor is associated with c-src kinase in rat liver.
The mechanism of action of the pituitary hormone PRL was studied in hepatocytes of lactating rats. PRL receptor immune complexes obtained from liver lysates have an associated tyrosine kinase activity. The tyrosine kinase has been identified in isolated hepatocytes as pp60c-src. Incubation of hepatocytes with PRL induces the association of PRL receptor with pp60c-src and the resultant stimulation of its tyrosine kinase activity. Furthermore, PRL stimulates the gene expression of c-fos, c-jun, and c-src. All of these findings support the idea that the pp60c-src tyrosine kinase participates in the early steps of the PRL intracellular signaling that promotes cell growth in liver cells.
Berlanga
J J
JJ
Departamento de Biologia Molecular, Centro de Biologia Molecular Severo Ochoa, Madrid, Spain.
Fresno Vara
J A
JA
Martín-Pérez
J
J
García-Ruiz
J P
JP
D000818:Animals; D000936:Antigen-Antibody Complex / Q000302:isolation & purification; D002455:Cell Division; D005260:Female; D005786:Gene Expression Regulation; D007774:Lactation; D008099:Liver / Q000378:metabolism*; D046911:Macromolecular Substances; D010766:Phosphorylation; D011388:Prolactin / Q000494:pharmacology; D011499:Protein Processing, Post-Translational; D016760:Proto-Oncogene Proteins c-fos / Q000096:biosynthesis / Q000235:genetics; D016755:Proto-Oncogene Proteins c-jun / Q000096:biosynthesis / Q000235:genetics; D016392:Proto-Oncogene Proteins pp60(c-src) / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar; D020794:Receptor Protein-Tyrosine Kinases / Q000302:isolation & purification / Q000378:metabolism*; D011981:Receptors, Prolactin / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology*
D000936:Antigen-Antibody Complex; D046911:Macromolecular Substances; D016760:Proto-Oncogene Proteins c-fos; D016755:Proto-Oncogene Proteins c-jun; D012333:RNA, Messenger; D011981:Receptors, Prolactin; D011388:Prolactin; D020794:Receptor Protein-Tyrosine Kinases; D016392:Proto-Oncogene Proteins pp60(c-src)