Suppression of azoxymethane-induced rat colon carcinogenesis by dietary administration of naturally occurring xanthophylls astaxanthin and canthaxanthin during the postinitiation phase.
The modulating effects of dietary feeding of two xanthophylls, astaxanthin (AX) and canthaxanthin (CX) during the postinitiation phase on colon carcinogenesis initiated with azoxymethane (AOM) were investigated in male F344 rats. Animals were initiated with AOM by weekly s.c. injections of 15 mg/kg body wt for 3 weeks and then they were fed the diets containing AX or CX at concentrations of 100 and 500 p.p.m. for 34 weeks. The others contained the groups of rats treated with AX or CX alone and untreated. At the end of the study (week 37), the incidence and multiplicity of neoplasms (adenoma and adenocarcinoma) in the large intestine of rats initiated with AOM and followed by AX or CX containing diet at a high dose (500 p.p.m.) were significantly smaller than those of rats given AOM alone (P < 0.001). In addition, AX or CX feeding significantly inhibited the development of aberrant crypt foci induced by AOM. Dietary exposure to AX or CX also decreased cell proliferation activity as revealed by measuring 5'-bromodeoxyuridine-labeling index as crypt cells, colonic mucosal ornithine decarboxylase activity and blood polyamine levels. These results indicate that AX and CX are possible chemopreventers for carcinogenesis of colon in addition to urinary bladder and oral cavity and such effects may be partly due to suppression of cell proliferation.
Tanaka
T
T
First Department of Pathology, Gifu University School of Medicine, Japan.
Kawamori
T
T
Ohnishi
M
M
Makita
H
H
Mori
H
H
Satoh
K
K
Hara
A
A
D000818:Animals; D001397:Azoxymethane / Q000037:antagonists & inhibitors; D001973:Bromodeoxyuridine; D016644:Canthaxanthin / Q000008:administration & dosage / Q000627:therapeutic use*; D002273:Carcinogens / Q000037:antagonists & inhibitors; D002338:Carotenoids / Q000008:administration & dosage / Q000627:therapeutic use*; D003106:Colon / Q000201:enzymology; D003110:Colonic Neoplasms / Q000139:chemically induced / Q000453:epidemiology / Q000517:prevention & control*; D004032:Diet; D015994:Incidence; D007413:Intestinal Mucosa / Q000201:enzymology; D008297:Male; D009955:Ornithine Decarboxylase / Q000378:metabolism; D011073:Polyamines / Q000097:blood; D051381:Rats; D011916:Rats, Inbred F344; D024341:Xanthophylls; D019207:beta Carotene / Q000031:analogs & derivatives*
D002273:Carcinogens; D011073:Polyamines; D024341:Xanthophylls; D019207:beta Carotene; D002338:Carotenoids; D016644:Canthaxanthin; C005948:astaxanthine; D009955:Ornithine Decarboxylase; D001973:Bromodeoxyuridine; D001397:Azoxymethane