DNA-nuclear matrix interactions analyzed by cross-linking reactions in intact nuclei from avian liver.
To detect the interactions of DNA with the nuclear matrix proteins, DNA-protein cross-linkages were induced in intact nuclei from chicken liver by the use of cis-diammine dichloroplatinum. Methods have been devised for fast purification both of the proteins and of the DNA fragments involved in the cross-linked complexes. By Southern-Western blotting a number of matrix proteins isolated from the complexes have been shown to recognize specifically DNA sequences present in the cross-linked DNA fragments. This experimental approach not only allows to identify the nuclear matrix-DNA interactions existing in the nucleus before its disruption, but also provides a preparation of matrix proteins enriched in those species which are involved in such interactions and which can therefore be detected with high sensitivity.
Ferraro
A
A
Department of Biochemical Sciences A. Rossi Fanelli, University La Sapienza, Rome, Italy.
Eufemi
M
M
Cervoni
L
L
Altieri
F
F
Turano
C
C
D000818:Animals; D034961:Antigens, Nuclear; D002467:Cell Nucleus / Q000187:drug effects* / Q000378:metabolism; D002645:Chickens; D002945:Cisplatin; D003432:Cross-Linking Reagents; D004268:DNA-Binding Proteins / Q000378:metabolism*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D009687:Nuclear Proteins / Q000378:metabolism*
D034961:Antigens, Nuclear; D003432:Cross-Linking Reagents; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D002945:Cisplatin