Upregulated expression of the genes encoding translation initiation factors eIF-4E and eIF-2alpha in transformed cells.
Increased protein synthesis is necessary for the transition of cells from quiescence to proliferation. It is shown in this paper that the induction of expression of the translation initiation factor eIF-4E in normal cells requires serum growth factors, while this requirement is abrogated in tumor cells analyzed in this study. Further, the expression of eIF-4E and eIF-2alpha is increased in c-myc, v-src, and v-abl-transformed cells. It is demonstrated that an increase in c-myc function leads to elevated expression of eIF-4E and eIF-2alpha, increases in net protein synthesis and cell proliferation. It may be suggested that constitutive activation of translational machinery may be one common mechanism by which various oncogenes exert their transforming function.
Rosenwald
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Harvard-Massachusetts Institute of Technology Division of Health Sciences and Technology, Cambridge, 02139, USA.
D016475:3T3 Cells / Q000378:metabolism / Q000502:physiology; D000818:Animals; D002453:Cell Cycle / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D016895:Culture Media, Serum-Free; D015852:Eukaryotic Initiation Factor-2 / Q000096:biosynthesis* / Q000235:genetics*; D039561:Eukaryotic Initiation Factor-4E; D005347:Fibroblasts / Q000378:metabolism / Q000502:physiology; D005786:Gene Expression Regulation; D016313:Genes, abl; D016259:Genes, myc; D016391:Genes, src; D006133:Growth Substances / Q000097:blood / Q000494:pharmacology; D051379:Mice; D010448:Peptide Initiation Factors / Q000096:biosynthesis* / Q000235:genetics*; D014176:Protein Biosynthesis; D016271:Proto-Oncogene Proteins c-myc / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D015854:Up-Regulation
D016895:Culture Media, Serum-Free; D015852:Eukaryotic Initiation Factor-2; D039561:Eukaryotic Initiation Factor-4E; D006133:Growth Substances; D010448:Peptide Initiation Factors; D016271:Proto-Oncogene Proteins c-myc