Genotoxic effects of bistratene A on human lymphocytes.
Bistratene A, a toxin isolated from the colonial ascidian Lissoclinum bistratum causes a decrease in mitotic index and retardation of lymphocyte proliferation kinetics when it is added at 48 h to 72-h human lymphocyte cultures. In the same cultures, the incidence of sister chromatid exchanges was not altered by this compound. We also observed an increase in the number of polyploid cells in the cultures, and alterations of the beta-tubulin organization by immunocytochemistry with an antibody against beta-tubulin. Bistratene A induces DNA damage in a dose-dependent fashion in leukocytes, as measured by the alkaline single cell gel electrophoresis assay. These results show that bistratene A interferes with microtubule assembly, is cytotoxic and cytostatic, and that it causes DNA damage.
Rojas
E
E
Departamento de Genética y Toxicología Ambiental, Instituto de Investigaciones Biomedicas, Mexico.
Valverde
M
M
Vega
L
L
Salvador
A
A
Ramirez
P
P
Herrera
L A
LA
Watters
D
D
Lavin
M F
MF
Ostrosky-Wegman
P
P
D000081:Acetamides; D008841:Actin Cytoskeleton / Q000187:drug effects; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004247:DNA / Q000378:metabolism; D004249:DNA Damage; D003703:Demecolcine / Q000494:pharmacology; D004988:Ethers, Cyclic / Q000633:toxicity*; D006801:Humans; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008387:Marine Toxins / Q000633:toxicity*; D008870:Microtubules / Q000187:drug effects; D010835:Phytohemagglutinins / Q000494:pharmacology; D011123:Polyploidy; D011714:Pyrans; D012854:Sister Chromatid Exchange / Q000187:drug effects / Q000235:genetics; D013141:Spiro Compounds; D014404:Tubulin / Q000378:metabolism; D014561:Urochordata
D000081:Acetamides; D004988:Ethers, Cyclic; D008387:Marine Toxins; D010835:Phytohemagglutinins; D011714:Pyrans; D013141:Spiro Compounds; D014404:Tubulin; C059975:bistratene A; D004247:DNA; D003703:Demecolcine