Ozone induction of cytokine-induced neutrophil chemoattractant (CINC) and nuclear factor-kappa b in rat lung: inhibition by corticosteroids.
We determined in rat lung whether ozone exposure was associated with the expression of the chemokine, cytokine-induced neutrophil chemoattractant (CINC), and of the transcription factor, NF-kappa B. CINC mRNA expression peaked at 2 h after cessation of ozone exposure, and returned to basal levels by 24 h. DNA-binding activity of NF-kappa B showed a marked increase after ozone, maximal at 2 h. Dexamethasone inhibited CINC mRNA and NF-kappa B expression, together with neutrophilic inflammation. Our data supports the concept that ozone leads to NF-kappa B activation which increases CINC mRNA expression. These series of events could lead to neutrophilic inflammation.
Haddad
E B
EB
Department of Thoracic Medicine, National Heart and Lung Institute, Imperial College, London, UK.
Salmon
M
M
Koto
H
H
Barnes
P J
PJ
Adcock
I
I
Chung
K F
KF
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D001483:Base Sequence; D018925:Chemokines / Q000378:metabolism*; D019743:Chemokines, CXC; D002630:Chemotactic Factors / Q000737:chemistry / Q000378:metabolism*; D003907:Dexamethasone / Q000494:pharmacology*; D006131:Growth Inhibitors / Q000737:chemistry / Q000378:metabolism*; D006133:Growth Substances / Q000737:chemistry / Q000378:metabolism*; D007249:Inflammation; D036341:Intercellular Signaling Peptides and Proteins; D008168:Lung / Q000378:metabolism*; D008969:Molecular Sequence Data; D016328:NF-kappa B / Q000378:metabolism*; D010126:Ozone / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D011914:Rats, Inbred BN; D013047:Specific Pathogen-Free Organisms
D000893:Anti-Inflammatory Agents; D018925:Chemokines; D019743:Chemokines, CXC; D002630:Chemotactic Factors; D006131:Growth Inhibitors; D006133:Growth Substances; D036341:Intercellular Signaling Peptides and Proteins; D016328:NF-kappa B; D012333:RNA, Messenger; D010126:Ozone; D003907:Dexamethasone