title: The heat shock and ethanol stress responses of yeast exhibit extensive similarity and functional overlap.
abstract: Sublethal heat and ethanol exposure induce essentially identical stress responses in yeast. These responses are characterized by the induction of heat shock proteins, proteins requiring a temperature above about 35 degrees C or ethanol levels above a threshold level of 4-6% (v/v) for strong induction. One induced protein, Hsp104, contributes to both thermotolerance and ethanol tolerance, while others are anti-oxidant enzymes. Heat and ethanol stress cause similar changes to plasma membrane protein composition, reducing the levels of plasma membrane H(+)-ATPase protein and inducing the plasma membrane-associated Hsp30. Both stresses also stimulate the activity of the fraction of H(+)-ATPase remaining in the plasma membrane. The resulting enhancement to catalysed proton efflux from the cell represents a considerable energy demand, yet may help to counteract the adverse effects for homeostasis of the increased membrane permeability that results from stress.
authors.affiliation: Department of Biochemistry and Molecular Biology, University College London, UK.
authors.forename: P W
authors.initials: PW
authors.lastname: Piper
mesh_terms: D000975:Antioxidants / Q000378:metabolism; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D000431:Ethanol / Q000633:toxicity*; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects; D005800:Genes, Fungal / Q000187:drug effects; D006360:Heat-Shock Proteins / Q000096:biosynthesis / Q000235:genetics; D006358:Hot Temperature; D008563:Membrane Lipids / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000187:drug effects; D006180:Proton-Translocating ATPases / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism*; D014199:Trehalose / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D006360:Heat-Shock Proteins; D008563:Membrane Lipids; D000431:Ethanol; D014199:Trehalose; D006180:Proton-Translocating ATPases