A new effective method for the evaluation of glycated intact plasma proteins in diabetic subjects.
The molecular weights of plasma proteins from healthy subjects and from patients with well-or badly-controlled diabetes mellitus have been determined by use of a matrix-assisted laser desorption ionization method, representing a highly accurate technique for the determination of the molecular weight of large biomolecules. Using this approach, different molecular weights of human serum albumin have been found for healthy (66,572-66,694 dalton) and diabetic (66,785-68,959 dalton) subjects. Such differences can be rationalized as being due to the different number of glucose molecules condensed on the protein and/or their further oxidation products; in the case of our diabetic patients this number is in the range of 1.4-14.8. The data show the high validity and specificity of the technique, which allows us to evaluate, without any protein degradation procedure, the number of glucose molecules condensed on a specific protein and ascertain the relationship of this number to the physiopathogenetic conditions of the subjects studied.
Lapolla
A
A
Istituto di Medicina Interna, Cattedra Malattie del Metabolismo, Università di Padova, Italy.
Fedele
D
D
Seraglia
R
R
Catinella
S
S
Baldo
L
L
Aronica
R
R
Traldi
P
P
D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000097:blood; D001786:Blood Glucose / Q000378:metabolism*; D001798:Blood Proteins / Q000032:analysis*; D003924:Diabetes Mellitus, Type 2 / Q000097:blood* / Q000503:physiopathology; D005260:Female; D006442:Glycated Hemoglobin / Q000032:analysis; D006023:Glycoproteins; D006801:Humans; D008239:Lysine / Q000031:analogs & derivatives / Q000097:blood; D008297:Male; D008875:Middle Aged; D012016:Reference Values; D000093422:Glycated Serum Proteins
D015415:Biomarkers; D001786:Blood Glucose; D001798:Blood Proteins; D006442:Glycated Hemoglobin; D006023:Glycoproteins; D008239:Lysine; D000093422:Glycated Serum Proteins; C018948:furosine