title: [Effect of the topography of the signal peptidase site on the effectiveness of secretion of recombinant human granulocyte-macrophage colony-stimulating factor into Escherichia coli periplasm].
abstract: Synthesis of an artificial gene encoding the signal peptide of the Yersinia pestis capsule antigen (Caf1) was accomplished. A set of plasmids coding for hybrid proteins in which a modified sequence of the Caf1 signal peptide is connected to the amino acid sequence of the mature granulocyte-macrophage colony stimulating factor (GM-CSF) were constructed. Topography of the cleavage site of signal proteases was studied. The presence of an arginine residue within the N-terminal part of the mature human GM-CSF was shown to hinder the proper processing and translocation of the precursor through periplasmic membrane. A number of E. coli strains secreting biologically active mutants of human GM-CSF were obtained.
authors.forename: L E
authors.initials: LE
authors.lastname: Petrovskaia
authors.forename: E A
authors.initials: EA
authors.lastname: Kriukov
authors.forename: S A
authors.initials: SA
authors.lastname: Iakimov
authors.forename: A N
authors.initials: AN
authors.lastname: Vul'fson
authors.forename: R A
authors.initials: RA
authors.lastname: Alibaeva
authors.forename: L N
authors.initials: LN
authors.lastname: Shingarova
authors.forename: A A
authors.initials: AA
authors.lastname: Guzaev
authors.forename: V M
authors.initials: VM
authors.lastname: Abramov
authors.forename: V G
authors.initials: VG
authors.lastname: Korobko
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D021382:Protein Sorting Signals / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D021382:Protein Sorting Signals; D011994:Recombinant Proteins; D016178:Granulocyte-Macrophage Colony-Stimulating Factor