Possible sources of endothelin-1 in damaged rat brain.
We investigated the sources of endothelin-1 (ET-1) detected in the damaged brain tissue of rats after cold injury. ET-1 in the injured sites was increased 1-5 days after the injury. Physiologic observations revealed that breakdown of the blood-brain barrier occurred and that plasma-derived albumin exuded into the brain parenchyma after cold injury. Radiolabeled ET-1, which was injected into the left cardiac ventricle, was detected in the tissue at the injury site, and ET-1-like immunoreactivity was observed in the brain capillaries in the damaged tissues. TGF-beta 1, which is a tissue regeneration factor, potently induced the expression of ET-1 mRNA in cultured astrocytes. These results suggest that ET-1 is produced through a variety of ways in the damaged brain.
Yamada
G
G
Department of Pharmacology, University of Tsukuba, Ibaraki, Japan.
Hama
H
H
Kasuya
Y
Y
Masaki
T
T
Goto
K
K
D000818:Animals; D001921:Brain / Q000502:physiology; D001923:Brain Chemistry; D001930:Brain Injuries / Q000378:metabolism*; D002478:Cells, Cultured; D003080:Cold Temperature; D016232:Endothelins / Q000032:analysis* / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D012038:Regeneration
D016232:Endothelins; D012333:RNA, Messenger