The PR-1a promoter contains a number of elements that bind GT-1-like nuclear factors with different affinity.
The 900 bp promoter region of the tobacco PR-1a gene was divided into eight fragments using PCR. The fragments were tested for their ability to bind to nuclear factors isolated from tobacco leaf. Band shift assays demonstrated that all but one of the fragments specifically interacted with nuclear proteins. From competition experiments it was determined that the same nuclear factors bind various promoter fragments with different affinity. Moreover, efficient competition with a synthetic tetramer of box II of the rbcS promoter indicated that GT-1-like nuclear factors are involved in these interactions. Furthermore, in comparison to extracts from untreated plants, nuclear protein preparations from tobacco mosaic virus-infected tobacco showed a reduced GT-1 binding activity. These results will be discussed in relation to induced PR-1a gene expression.
Buchel
A S
AS
Institute of Molecular Plant Sciences, Gorlaeus Laboratories, Leiden University, Netherlands.
Molenkamp
R
R
Bol
J F
JF
Linthorst
H J
HJ
D001483:Base Sequence; D001667:Binding, Competitive; D018744:DNA, Plant / Q000235:genetics; D004268:DNA-Binding Proteins / Q000378:metabolism*; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics*; D010947:Plants, Toxic; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D014026:Nicotiana / Q000235:genetics; D014157:Transcription Factors
D018744:DNA, Plant; D004268:DNA-Binding Proteins; C077926:GT-1 transcription factor; D009687:Nuclear Proteins; D010940:Plant Proteins; D014157:Transcription Factors; C053376:pathogenesis-related proteins, plant