A simple touch-down polymerase chain reaction for the detection of canine parvovirus and feline panleukopenia virus in feces.
A polymerase chain reaction (PCR) assay is described for the detection of parvovirus in feces of dogs and cats. A touch-down protocol was used which enabled the specific amplification of virion DNA from feces after a fast and simple boiling pretreatment. The sensitivity of PCR was as high as ten infectious particles per reaction which corresponds to a titer of about 10(3) infectious particles per gram of unprocessed feces. This renders the PCR about 10- to 100-fold more sensitive than electron microscopy, the standard method for parvovirus diagnosis. The very rapid and simple sample preparation recommends this PCR assay as an alternative technique for routine parvovirus diagnosis.
Schunck
B
B
Medical Animal Clinic, Ludwig Maximilians University Munich, Germany.
Kraft
W
W
Truyen
U
U
D000818:Animals; D001483:Base Sequence; D002415:Cats; D002460:Cell Line; D017931:DNA Primers; D004283:Dog Diseases / Q000175:diagnosis / Q000821:virology; D004285:Dogs; D005243:Feces / Q000821:virology*; D005254:Feline Panleukopenia / Q000175:diagnosis / Q000821:virology; D028301:Feline Panleukopenia Virus / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008969:Molecular Sequence Data; D010322:Parvoviridae Infections / Q000662:veterinary / Q000821:virology; D017993:Parvovirus, Canine / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
D017931:DNA Primers