title: Sequence, tissue distribution and developmental changes in rat intestinal oligopeptide transporter.
abstract: Complementary DNA clones encoding the rat PepT1 small-intestinal oligopeptide transporter were isolated from a jejunal library by cross-hybridization with a rabbit PepT1 cDNA probe. The cDNA sequence indicates that rat PepT1 is composed of 710 amino acids and shows 77% and 83% amino acid sequence identity with rabbit and human PepT1, respectively. Northern blot analysis detected rat PepT1 mRNA in the small intestine and kidney. Intestinal PepT1 mRNA levels were highest in 4-day old rats, and then decreased reaching the adult level by day 28 after birth. These results indicate that the expressions of PepT1 gene change markedly during development.
authors.affiliation: Department of Clinical Nutrition, School of Medicine, Tokushima University, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Miyamoto
authors.forename: T
authors.initials: T
authors.lastname: Shiraga
authors.forename: K
authors.initials: K
authors.lastname: Morita
authors.forename: H
authors.initials: H
authors.lastname: Yamamoto
authors.forename: H
authors.initials: H
authors.lastname: Haga
authors.forename: Y
authors.initials: Y
authors.lastname: Taketani
authors.forename: I
authors.initials: I
authors.lastname: Tamai
authors.forename: Y
authors.initials: Y
authors.lastname: Sai
authors.forename: A
authors.initials: A
authors.lastname: Tsuji
authors.forename: E
authors.initials: E
authors.lastname: Takeda
mesh_terms: D000367:Age Factors; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism*; D018076:DNA, Complementary; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D007421:Intestine, Small / Q000254:growth & development / Q000378:metabolism*; D007583:Jejunum / Q000254:growth & development / Q000378:metabolism; D007668:Kidney / Q000254:growth & development / Q000378:metabolism; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000378:metabolism*; D000074011:Peptide Transporter 1; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011817:Rabbits; D051381:Rats; D017207:Rats, Sprague-Dawley; D017386:Sequence Homology, Amino Acid; D027981:Symporters; D014018:Tissue Distribution
chemical_list: D002352:Carrier Proteins; D018076:DNA, Complementary; D009842:Oligopeptides; D000074011:Peptide Transporter 1; D012333:RNA, Messenger; C082186:SLC15A1 protein, human; C489940:Slc15a1 protein, rat; D027981:Symporters