title: Expression, purification, and assays of Gdi1p from recombinant Escherichia coli.
authors.affiliation: Onyx Pharmaceuticals, Richmond, California 94806, USA.
authors.forename: M D
authors.initials: MD
authors.lastname: Garrett
authors.forename: P J
authors.initials: PJ
authors.lastname: Novick
mesh_terms: D000818:Animals; D002852:Chromatography, Ion Exchange / Q000379:methods; D003001:Cloning, Molecular / Q000379:methods; D004330:Drosophila / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D004926:Escherichia coli; D019204:GTP-Binding Proteins / Q000032:analysis / Q000096:biosynthesis* / Q000302:isolation & purification*; D005982:Glutathione Transferase / Q000096:biosynthesis; D020730:Guanine Nucleotide Dissociation Inhibitors; D016244:Guanosine 5'-O-(3-Thiotriphosphate) / Q000378:metabolism; D006153:Guanosine Diphosphate / Q000378:metabolism; D007700:Kinetics; D008322:Mammals; D010957:Plasmids; D011865:Radioisotope Dilution Technique; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D011994:Recombinant Proteins / Q000032:analysis / Q000096:biosynthesis* / Q000302:isolation & purification; D015183:Restriction Mapping; D014316:Tritium
chemical_list: C086796:GDP dissociation inhibitor 1; D020730:Guanine Nucleotide Dissociation Inhibitors; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; D014316:Tritium; D006153:Guanosine Diphosphate; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D005982:Glutathione Transferase; D019204:GTP-Binding Proteins