title: Production of heparin-binding epidermal growth factor-like growth factor (HB-EGF) at sites of thermal injury in pediatric patients.
abstract: Fluids that accumulate at wound sites may be an important reservoir of growth factors that promote the normal wound healing response. The presence of heparin-binding growth factors was studied in burn wound fluid (BWF) from 45 pediatric patients who had sustained partial thickness burns. One of the growth factors present was similar to platelet-derived growth factor (PDGF) based on its heparin affinity, inhibition of bioactivity by a PDGF antiserum, and detection in a PDGF-AB enzyme-linked immunosorbent assay. A second growth factor was identified as heparin-binding epidermal growth factor-like growth factor (HB-EGF) based on its heparin affinity, competition with 125I-labeled epidermal growth factor (EGF) for EGF receptor binding, and recognition in biological assays and Western blots by two HB-EGF antisera. Amino acid sequence analysis of one form of this second growth factor verified its identity as an N-terminally truncated form of HB-EGF. Immunohistochemical analysis of partial thickness burns demonstrated the presence of HB-EGF in the advancing epithelial margin, islands of regenerating epithelium within the burn wound, and in the duct and proximal tubules of eccrine sweat glands. HB-EGF in the surface epithelium of burn wounds was uniformally distributed, whereas it was restricted to the basal epithelium in nonburned skin. These data support a role for PDGF and HB-EGF in burn wound healing and suggest that the response to injury includes deposition of HB-EGF and PDGF into blister fluid and a redistribution of HB-EGF in the surface epithelium near the wound site.
authors.affiliation: Department of Surgery, Ohio State University, Columbus, USA.
authors.forename: D W
authors.initials: DW
authors.lastname: McCarthy
authors.forename: M T
authors.initials: MT
authors.lastname: Downing
authors.forename: D R
authors.initials: DR
authors.lastname: Brigstock
authors.forename: M H
authors.initials: MH
authors.lastname: Luquette
authors.forename: K D
authors.initials: KD
authors.lastname: Brown
authors.forename: M S
authors.initials: MS
authors.lastname: Abad
authors.forename: G E
authors.initials: GE
authors.lastname: Besner
mesh_terms: D000293:Adolescent; D001826:Body Fluids / Q000378:metabolism; D002056:Burns / Q000378:metabolism*; D002648:Child; D002675:Child, Preschool; D004815:Epidermal Growth Factor / Q000737:chemistry / Q000378:metabolism*; D006493:Heparin / Q000378:metabolism; D066257:Heparin-binding EGF-like Growth Factor; D006801:Humans; D007150:Immunohistochemistry; D007223:Infant; D036341:Intercellular Signaling Peptides and Proteins; D010982:Platelet-Derived Growth Factor / Q000378:metabolism; D012016:Reference Values; D014018:Tissue Distribution
chemical_list: C587883:HBEGF protein, human; D066257:Heparin-binding EGF-like Growth Factor; D036341:Intercellular Signaling Peptides and Proteins; D010982:Platelet-Derived Growth Factor; D004815:Epidermal Growth Factor; D006493:Heparin