Use of digoxigenin-labelled probes for the detection of B19 parvovirus DNA in batches of blood products.
Being thermostable and non lipid-enveloped, the B19 parvovirus cannot be destroyed by the chemical and physical treatments presently used to inactivate lipid-enveloped viruses such as HIV, hepatitis C and B viruses in plasma derivatives. A polymerase chain reaction assay was used to detect B19 parvovirus DNA. Amplified products were detected through a non-radioactive technology based on the use of a digoxigenin-labelled probe. B19 parvovirus DNA was detected in 20% of the studied batches of factor coagulation concentrates and in none of the albumin batches.
Lefrère
J J
JJ
Institut National de Transfusion Sanguine, Hôpital Saint-Antoine, Paris, France.
Mariotti
M
M
D001769:Blood / Q000821:virology*; D001779:Blood Coagulation Factors / Q000302:isolation & purification; D004279:DNA, Viral / Q000097:blood / Q000235:genetics; D004076:Digoxigenin; D016731:Erythema Infectiosum / Q000175:diagnosis / Q000635:transmission; D005069:Evaluation Studies as Topic; D006801:Humans; D015336:Molecular Probe Techniques / Q000706:statistics & numerical data; D015335:Molecular Probes; D016732:Parvovirus B19, Human / Q000235:genetics* / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000706:statistics & numerical data; D012680:Sensitivity and Specificity; D012709:Serum Albumin / Q000302:isolation & purification; D014766:Viremia / Q000175:diagnosis
D001779:Blood Coagulation Factors; D004279:DNA, Viral; D015335:Molecular Probes; D012709:Serum Albumin; D004076:Digoxigenin