Liquid chromatographic assay using a microcolumn coupled to a U-shaped optical cell for high-sensitivity ultraviolet absorbance detection of oxcarbazepine and its major metabolite in microdialysates.
An isocratic LC assay using a microcolumn (800 microns I.D.) coupled to a U-shaped optical flow cell (cell volume 70 nl; optical path length 8 mm) for high-sensitivity UV absorbance is described for the detection of oxcarbazepine and its major and active metabolite, 10,11-dihydro-10-hydroxycarbamazepine in microdialysates. Using the combination microcolumn-capillary UV detector, a ten-fold increase in sensitivity was obtained resulting in a limit of detection of 10 pg/10 microliters. This assay is sufficiently sensitive to allow quantification of drug and metabolite in 10-microliters aliquots of rat blood and hippocampus microdialysates, using carbamazepine-10,11-epoxide as external standard.
Van Belle
K
K
Department of Pharmaceutical Chemistry and Drug Analysis, Vrije Universiteit Brussels, Belgium.
Verfaillie
I
I
Ebinger
G
G
Michotte
Y
Y
D000818:Animals; D000927:Anticonvulsants / Q000032:analysis* / Q000097:blood; D002220:Carbamazepine / Q000031:analogs & derivatives* / Q000032:analysis / Q000097:blood; D002853:Chromatography, Liquid; D015314:Dialysis Solutions / Q000737:chemistry; D006624:Hippocampus / Q000737:chemistry / Q000378:metabolism; D008297:Male; D017551:Microdialysis; D000078330:Oxcarbazepine; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
C039775:10,11-dihydro-10-hydroxycarbamazepine; D000927:Anticonvulsants; D015314:Dialysis Solutions; D002220:Carbamazepine; D000078330:Oxcarbazepine