[Use of the method of lanthanide immunofluorescence analysis for detection of lymphocyte Fc-receptors].
Interaction of lymphocyte Fc-receptors (FcR) with the conjugate, containing immunoglobulin G (IgG) labeled with chelating compound of derivative diethylenetriaminepentaacetic acid (DTPA)-DTPA-Eu, was investigated. For optimal conditions in performing lanthanide immunofluorescence analysis (LIFA) with minimal quantity of lymphocyte, i. e. 2 x 10(6) in ml and concentrate for conjugate 10(-8) - 10(-12) pH was found.
Miliukene
V V
VV
Radziavichene
Iu A
IuA
Dedinene
Ia L
IaL
D000818:Animals; D002417:Cattle; D002614:Chelating Agents; D005260:Female; D005455:Fluorescent Antibody Technique; D006863:Hydrogen-Ion Concentration; D007074:Immunoglobulin G / Q000097:blood; D008214:Lymphocytes / Q000737:chemistry*; D008674:Metals, Rare Earth / Q000737:chemistry*; D004369:Pentetic Acid; D011961:Receptors, Fc / Q000032:analysis*
D002614:Chelating Agents; D007074:Immunoglobulin G; D008674:Metals, Rare Earth; D011961:Receptors, Fc; D004369:Pentetic Acid