Effect of amino acids on rapamycin biosynthesis by Streptomyces hygroscopicus.
In a chemically defined medium containing aspartate, arginie and histidine to support good growth, addition of L-lysine stimulated rapamycin production by 150%. This was probably due to its conversion to pipecolic acid, a rapamycin precursor. Phenylalanine and methionine interfered in rapamycin production by unknown mechanisms.
Cheng
Y R
YR
Fermentation Microbiology Laboratory, Massachusetts Institute of Technology, Cambridge 02139, USA.
Fang
A
A
Demain
A L
AL
D000596:Amino Acids / Q000494:pharmacology*; D000935:Antifungal Agents / Q000096:biosynthesis*; D003470:Culture Media / Q000494:pharmacology; D008239:Lysine / Q000494:pharmacology; D008715:Methionine / Q000494:pharmacology; D010649:Phenylalanine / Q000494:pharmacology; D010875:Pipecolic Acids / Q000378:metabolism; D011090:Polyenes / Q000378:metabolism*; D000644:Quaternary Ammonium Compounds / Q000378:metabolism; D020123:Sirolimus; D013302:Streptomyces / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism
D000596:Amino Acids; D000935:Antifungal Agents; D003470:Culture Media; D010875:Pipecolic Acids; D011090:Polyenes; D000644:Quaternary Ammonium Compounds; D010649:Phenylalanine; D008715:Methionine; C031345:pipecolic acid; D008239:Lysine; D020123:Sirolimus