title: Lipo-oligosaccharide of Campylobacter lari strain PC 637. Structure of the liberated oligosaccharide and an associated extracellular polysaccharide.
abstract: Lipo-oligosaccharide from phenol-water extraction of cells of Campylobacter lari strain PC 637 was separated as a water-insoluble gel of low relative molecular mass (M(r)) from a water-soluble extracellular polysaccharide of high M(r). Structural investigations were performed on the lipo-oligosaccharide and the extracellular polysaccharide, variously using 1H, 13C, and 31P NMR spectroscopy, linkage analysis, and fast atom bombardment-mass spectrometry of permethylated derivatives of the glycans and their products of chemical and enzymic degradation. The following structures are proposed for the highly branched oligosaccharide region: [formula: see text] and for the tetraglycosyl phosphate repeating unit of the extracellular polysaccharide: [formula; see text]
authors.affiliation: Department of Chemistry, York University, North York, Toronto, Ontario, Canada.
authors.forename: G O
authors.initials: GO
authors.lastname: Aspinall
authors.forename: M A
authors.initials: MA
authors.lastname: Monteiro
authors.forename: H
authors.initials: H
authors.lastname: Pang
authors.forename: L A
authors.initials: LA
authors.lastname: Kurjanczyk
authors.forename: J L
authors.initials: JL
authors.lastname: Penner
mesh_terms: D000116:Acetylgalactosamine; D002167:Campylobacter / Q000737:chemistry*; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D003837:Deoxy Sugars / Q000737:chemistry; D005690:Galactose / Q000032:analysis; D005947:Glucose / Q000032:analysis; D006026:Glycoside Hydrolases / Q000378:metabolism; D006539:Heptoses / Q000032:analysis; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008745:Methylation; D008969:Molecular Sequence Data; D011134:Polysaccharides / Q000737:chemistry; D011135:Polysaccharides, Bacterial / Q000737:chemistry*; D012091:Repetitive Sequences, Nucleic Acid; D013403:Sugar Phosphates / Q000737:chemistry
chemical_list: D003837:Deoxy Sugars; D006539:Heptoses; D008070:Lipopolysaccharides; D011134:Polysaccharides; D011135:Polysaccharides, Bacterial; D013403:Sugar Phosphates; C023023:lipid-linked oligosaccharides; D006026:Glycoside Hydrolases; D005947:Glucose; D000116:Acetylgalactosamine; D005690:Galactose