Immunolocalization of cystatin A in condylomatous and dysplastic lesions of the human uterine cervix: correlation with the presence and type of human papillomavirus infection.
Cystatin A is the major cysteine proteinase inhibitor in human squamous epithelia. We investigated the occurrence of cystatin A in normal, condylomatous, and dysplastic lesions of the cervix with or without human papillomavirus (HPV) infection. Cystatin A was detected by immunohistochemistry and HPV infection by DNA hybridization techniques. In the normal uterine cervix, cystatin A was seen throughout the epithelium, except in the basal and parabasal cell layers. In condylomatous lesions, the staining intensity was similar to that in normal epithelium. In low-grade cervical intraepithelial neoplasias (CINs), reduced staining was seen in the lower third of the epithelium; in high-grade CINs, a reduction in staining intensity was also seen in the middle and upper thirds. Cystatin A staining in epithelia and nuclei was negative in highly cellular and poorly differentiated CIN III. The cytoplasmic staining of cystatin A did not correlate with presence or type of HPV DNA. In the high-grade CINs infected with HPV types 16 and 18, however, cystatin A staining was more often confined to the nuclear compartment.
Pöllänen
R
R
Department of Pathology, University of Oulu, Finland.
Pyykkönen
K
K
Järvinen
M
M
Rinne
A
A
Läärä
E
E
Lehto
V P
VP
Räsänen
O
O
D015891:Cystatins / Q000032:analysis*; D004279:DNA, Viral / Q000032:analysis; D004848:Epithelium / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D027383:Papillomaviridae / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000473:pathology*; D012189:Retrospective Studies; D014412:Tumor Virus Infections / Q000473:pathology*; D002578:Uterine Cervical Dysplasia / Q000473:pathology* / Q000821:virology
D015891:Cystatins; D004279:DNA, Viral