title: Recombinant endotoxin-binding protein (rBPI23) attenuates endotoxin-induced circulatory changes in humans.
abstract: In the present study the protective effect of a recombinant endotoxin-binding protein rBPI23 on the circulatory changes in experimental endotoxemia in humans was investigated. In a controlled, blinded crossover study, eight volunteers were challenged twice with an intravenous bolus injection of endotoxin (40 EU/kg body weight), and concurrently received either rBPI23 (1 mg/kg) or placebo (human serum albumin, 0.2 mg/kg). Hemodynamic parameters were obtained non-invasively by means of M-mode, two-dimensional, and Doppler echocardiography. rBPI23 significantly reduced indices of the endotoxin-induced hyperdynamic circulation. rBPI23 treatment significantly reduced increase in cardiac index (P = 0.0156). rBPI23 treatment diminished the endotoxin-induced decrease in systemic vascular resistance index (P = 0.0304). rBPI23 did not prevent the endotoxin-induced rise in body temperature and systolic, diastolic and mean arterial pressure were not significantly different in the rBPI23- and placebo-treatment arm. Both treatment periods showed a small reduction in end diastolic and end systolic volumes. rBPI23 treatment slightly reduced the increase in M-mode ejection fraction and fractional shortening. These results indicate that rBPI23 is capable of attenuating the potentially deleterious circulatory effects of endotoxin in humans.
authors.affiliation: Department of Cardiology, University of Amsterdam, The Netherlands.
authors.forename: R J
authors.initials: RJ
authors.lastname: de Winter
authors.forename: M A
authors.initials: MA
authors.lastname: von der Möhlen
authors.forename: H
authors.initials: H
authors.lastname: van Lieshout
authors.forename: N
authors.initials: N
authors.lastname: Wedel
authors.forename: B
authors.initials: B
authors.lastname: Nelson
authors.forename: N
authors.initials: N
authors.lastname: Friedmann
authors.forename: B J
authors.initials: BJ
authors.lastname: Delemarre
authors.forename: S J
authors.initials: SJ
authors.lastname: van Deventer
mesh_terms: D000328:Adult; D001794:Blood Pressure; D002302:Cardiac Output; D004731:Endotoxins / Q000097:blood / Q000494:pharmacology*; D006339:Heart Rate; D006439:Hemodynamics; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000494:pharmacology* / Q000627:therapeutic use; D011994:Recombinant Proteins / Q000494:pharmacology; D013318:Stroke Volume; D013696:Temperature; D014655:Vascular Resistance
chemical_list: D004731:Endotoxins; D008565:Membrane Proteins; D011994:Recombinant Proteins; C025357:endotoxin binding proteins