Regulation of classical HLA class I genes in human choriocarcinoma cells by nuclear proteins binding to MHC class I regulatory elements.
OBJECTIVE
The regulation of classical HLA class I genes in choriocarcinoma have been reported.


METHODS
We determined whether four choriocarcinoma cell lines expressed classical HLA class I or HLA-G by a reverse transcription-polymerase chain reaction (RT-PCR) and studied the regulatory mechanism of classical class I using a gel mobility shift assay.


RESULTS
NUC1 and SCH expressed classical class I but not HLA-G. GCH1 and Jar did neither. Nuclear protein binding to the class I regulatory element (CRE) was detected in NUC1 and SCH. Interferon-gamma augmented both classical class I expression and the DNA-protein complex in NUC1. The DNA-protein complex was not observed in GCH1, and Jar showed a CRE-binding protein with different electrophoretic mobility and binding affinity from that of SCH and NUC1.


CONCLUSIONS
The CRE is one of the regulatory elements of classical HLA class I genes in choriocarcinoma cells.
Wakimoto
A
A
Department of Obstetrics and Gynecology, Osaka University Medical School, Japan.
Ohashi
K
K
Koyama
M
M
Kato
M
M
Tsutsui
T
T
Saji
F
F
Tanizawa
O
O
D001483:Base Sequence; D002822:Choriocarcinoma / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000276:immunology*; D005805:Genes, MHC Class I / Q000276:immunology*; D006680:HLA Antigens / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism*; D015395:Histocompatibility Antigens Class I / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000378:metabolism / Q000494:pharmacology*; D011247:Pregnancy; D011485:Protein Binding / Q000276:immunology; D012333:RNA, Messenger / Q000096:biosynthesis; D012045:Regulatory Sequences, Nucleic Acid / Q000276:immunology*; D014407:Tumor Cells, Cultured
D006680:HLA Antigens; D015395:Histocompatibility Antigens Class I; D009687:Nuclear Proteins; D012333:RNA, Messenger