title: Microsatellite-centromere mapping in the zebrafish (Danio rerio).
abstract: Ten (CA)n microsatellite simple sequence repeat (SSR) markers, 1, 2, 12, 14, 16, 18, 20, 22, 26, and 29, were used to show high chiasma interference and to determine centromere-marker map distances in the zebrafish (Danio rerio). Of these, SSR 12 exhibited no recombinant tetratypes among 175 half-tetrad embryos, placing this marker within 1 cM of the centromere of Linkage Group XVII. Fractions of heterozygous half-tetrads for the remaining nine markers ranged from 0.64 to 0.89. Of these, six recombinant fractions were more than 0.67 (P < 0.05), indicating strong chiasma interference during female meiosis in the zebrafish. Consistent with previous mapping data, SSRs 2 and 20 of Linkage Group VI were tightly linked. Half-tetrad analysis will allow the mapping of the remaining centromeres and may be useful in the mapping of new genes and mutations in the zebrafish.
authors.affiliation: Department of Pathology, Jake Gittlen Cancer Research Institute, Hershey, Pennsylvania, USA.
authors.forename: E J
authors.initials: EJ
authors.lastname: Kauffman
authors.forename: E E
authors.initials: EE
authors.lastname: Gestl
authors.forename: D J
authors.initials: DJ
authors.lastname: Kim
authors.forename: C
authors.initials: C
authors.lastname: Walker
authors.forename: J M
authors.initials: JM
authors.lastname: Hite
authors.forename: G
authors.initials: G
authors.lastname: Yan
authors.forename: P K
authors.initials: PK
authors.lastname: Rogan
authors.forename: S L
authors.initials: SL
authors.lastname: Johnson
authors.forename: K C
authors.initials: KC
authors.lastname: Cheng
mesh_terms: D000818:Animals; D002503:Centromere; D004276:DNA, Satellite / Q000235:genetics*; D005260:Female; D006579:Heterozygote; D006720:Homozygote; D015027:Zebrafish / Q000196:embryology / Q000235:genetics*
chemical_list: D004276:DNA, Satellite