Identification of a putative G protein-coupled receptor induced during activation-induced apoptosis of T cells.
During development, self-reactive immature thymocytes are clonally deleted in the thymus, a phenomenon which establishes T cell tolerance (negative selection). It has been shown that the deletion of self-reactive immature T cells in the thymus is mediated by apoptosis upon T cell receptor (TCR) engagement. Apoptosis of immature thymocytes mediated by the TCR requires the expression of a new set of genes. To define which genes are required during the TCR-mediated death of immature thymocytes, we sought to identify genes whose expression is increased during TCR-mediated cell death. Using the technique of differential mRNA display, we have identified a novel gene, TDAG8, which encodes a putative G protein-coupled receptor. The expression of TDAG8 is greatly induced upon activation of T cells by anti-TCR antibody or by phorbol 12-myristate 13-acetate plus ionomycin. The treatment of T cells with glucocorticoids also greatly induces the expression of TDAG8. In mice, TDAG8 is predominantly expressed in thymus and spleen. The tissue-specific expression of TDAG8 and the induction of its expression during cell death of T cells mediated by the TCR or glucocorticoids suggest that it may have a role in activation-induced cell death or differentiation of T cells.
Choi
J W
JW
Howard Hughes Medical Institute, The Rockefeller University, New York 10021, USA.
Lee
S Y
SY
Choi
Y
Y
D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis / Q000235:genetics / Q000276:immunology*; D001483:Base Sequence; D016923:Cell Death / Q000235:genetics / Q000276:immunology; D002478:Cells, Cultured; D003001:Cloning, Molecular; D019204:GTP-Binding Proteins / Q000235:genetics* / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000276:immunology; D008213:Lymphocyte Activation / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D011948:Receptors, Antigen, T-Cell / Q000096:biosynthesis* / Q000235:genetics; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000276:immunology*; D013950:Thymus Gland / Q000166:cytology
D011948:Receptors, Antigen, T-Cell; D019204:GTP-Binding Proteins