title: [Use of the method of lanthanide immunofluorescence analysis for detection of lymphocyte Fc-receptors].
abstract: Interaction of lymphocyte Fc-receptors (FcR) with the conjugate, containing immunoglobulin G (IgG) labeled with chelating compound of derivative diethylenetriaminepentaacetic acid (DTPA)-DTPA-Eu, was investigated. For optimal conditions in performing lanthanide immunofluorescence analysis (LIFA) with minimal quantity of lymphocyte, i. e. 2 x 10(6) in ml and concentrate for conjugate 10(-8) - 10(-12) pH was found.
authors.forename: V V
authors.initials: VV
authors.lastname: Miliukene
authors.forename: Iu A
authors.initials: IuA
authors.lastname: Radziavichene
authors.forename: Ia L
authors.initials: IaL
authors.lastname: Dedinene
mesh_terms: D000818:Animals; D002417:Cattle; D002614:Chelating Agents; D005260:Female; D005455:Fluorescent Antibody Technique; D006863:Hydrogen-Ion Concentration; D007074:Immunoglobulin G / Q000097:blood; D008214:Lymphocytes / Q000737:chemistry*; D008674:Metals, Rare Earth / Q000737:chemistry*; D004369:Pentetic Acid; D011961:Receptors, Fc / Q000032:analysis*
chemical_list: D002614:Chelating Agents; D007074:Immunoglobulin G; D008674:Metals, Rare Earth; D011961:Receptors, Fc; D004369:Pentetic Acid