[Effects of an hydrosoluble fraction from bovine bone (Ossopan) on murine osteoblasts in culture].
The effects of an hydrosoluble fraction of the bovine bone extract Ossopan on cultured murine osteoblasts were assessed in this study. The hydrosoluble fraction from Ossopan, obtained by acid/ethanol extraction, contained significant amounts of TGF-beta 1. Calvariae from new-born rats were cultured for 1 month in synthetic medium (DMEM) supplemented with 10% fetal calf serum to allow cell proliferation; immunocytochemistry studies performed with a rabbit serum raised against alkaline phosphatase indicated that only osteoblasts were present in cell cultures. Using an XTT-based colorimetric cell proliferation assay, we showed that the hydrosoluble fraction of the bovine bone extract dose-dependently reduced the proliferation of cultured osteoblasts. A similar inhibition was obtained with a recombinant TGF-beta 1. Thus, the inhibitory effect of the hydrosoluble fraction of Ossopan on osteoblast proliferation should be due to the cytokines contained in this preparation.
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Laboratoire de Biochimie des Membranes, INSERM CJF 92-07, Faculté de Pharmacie, Montpellier.
Marcelli
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Cousse
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Bali
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JP
D000818:Animals; D000831:Animals, Newborn; D001842:Bone and Bones / Q000737:chemistry*; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D017886:Durapatite / Q000008:administration & dosage / Q000494:pharmacology*; D000431:Ethanol / Q000008:administration & dosage / Q000494:pharmacology*; D066298:In Vitro Techniques; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects*; D051381:Rats; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000494:pharmacology; D012995:Solubility; D016212:Transforming Growth Factor beta / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology
D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; D000431:Ethanol; D017886:Durapatite