title: Sialic acid-binding lectins: submolecular specificity and interaction with sialoglycoproteins and tumour cells.
abstract: We examined the specificity of limulin, Limax flavus agglutinin (LFA) and Sambucus nigra agglutinin I (SNA I) at the submolecular level of sialic acid, and characterized their interactions with a panel of structurally distinct sialoglycoproteins. In haemagglutination inhibition assays NeuAc-alpha-glycosides were stronger inhibitors for limulin and LFA than native N-acetylneuraminic acid (NeuAc). The N-acetyl of NeuAc was crucial for binding to both lectins. N-thioacetylated NeuAc lost affinity for LFA, but still bound to limulin. Thus, distinct intermolecular interactions are involved in binding of sialic acid to the lectins. The glyceryl side chain was required for interaction with LFA, but not with limulin. SNA I specifically bound NeuAc alpha 2 --> 6Gal beta 1 --> 4Glc, but not monomeric sialic acids. Limulin and LFA strongly interacted with O-chain glycoproteins, whereas SNA I preferred N-chain proteins that carry NeuAc alpha 2 --> 6 residues. The lectins were compared with those from Cepaea hortensis and Tachypleus tridentatus (TTA) and to wheat-germ agglutinin, and were then used to probe tumour cell lines for cell surface sialylation. With the exception of TTA, all lectins interacted with the tumour cells. Limulin distinguished between the low (Eb) and highly (ESb) metastatic mouse lymphoma lines by selectively agglutinating sialidase-treated ESb cells.
authors.affiliation: Institute of Biochemistry II, University of Heidelberg, Federal Republic of Germany.
authors.forename: E
authors.initials: E
authors.lastname: Fischer
authors.forename: R
authors.initials: R
authors.lastname: Brossmer
mesh_terms: D000818:Animals; D002240:Carbohydrate Sequence; D002460:Cell Line; D003110:Colonic Neoplasms; D006384:Hemagglutination / Q000187:drug effects; D006385:Hemagglutination Inhibition Tests; D006386:Hemagglutination Tests; D006737:Horseshoe Crabs; D006801:Humans; D037102:Lectins; D016399:Lymphoma, T-Cell / Q000503:physiopathology*; D051379:Mice; D008811:Mice, Inbred DBA; D008969:Molecular Sequence Data; D009439:Neuraminidase / Q000494:pharmacology; D009844:Oligosaccharides / Q000494:pharmacology*; D037121:Plant Lectins; D054788:Ribosome Inactivating Proteins; D012794:Sialic Acids / Q000494:pharmacology; D012795:Sialoglycoproteins; D013329:Structure-Activity Relationship; D013379:Substrate Specificity; D014407:Tumor Cells, Cultured
chemical_list: D037102:Lectins; D009844:Oligosaccharides; D037121:Plant Lectins; C050600:Sambucus nigra lectins; D012794:Sialic Acids; D012795:Sialoglycoproteins; C015323:limulin; D009439:Neuraminidase; D054788:Ribosome Inactivating Proteins