Effects of cold ischemia time on the graft after orthotopic liver transplantation. A bile cytological study.
We conducted a daily analysis of bile cellularity in 16 orthotopic liver transplant patients fitted with a T-tube, and correlated the cytological parameters (number of cells per slide, and percentage of difference cell types) with the duration of cold ischemia time (CIT). Two groups were established: one comprised patients whose CIT was less than 7 hr (CIT averaged 345 min) and the other comprised patients with a CIT of more than 7 hr (CIT averaged 505 min). The control group consisted of 15 patients who had received cholecystectomy for biliary lithiasis and were fitted with a T-tube. All 3 groups showed the highest cell density on the 1st postoperative day (control: 53.3 +/- 15.5 cells/slide; short ischemia: 70 +/0 21.4 cells/slide; long ischemia: 158.8 +/- 53.2 cells/slide), which steadily ischemia group showed a higher cell density than did the control group for the first 2 days, although this was not significant. The long ischemia group showed the highest cell density, although only significantly for the first 2 days when compared with the controls, and basically at the expense of a increase in ductal epithelial cells. Our results show that prolonged cold ischemia causes an increase in bile cell density at the expense of ductal epithelial cells: the longer the preservation time, the greater the increase.
Carrasco
L
L
Department of Surgery, University of Hospital Virgen de la Arrixaca, Murcia, Spain.
Sanchez-Bueno
F
F
Sola
J
J
Ruiz
J M
JM
Ramirez
P
P
Robles
R
R
Rodriquez
J M
JM
Parrilla
P
P
D000293:Adolescent; D000328:Adult; D000368:Aged; D001646:Bile / Q000166:cytology*; D001652:Bile Ducts / Q000166:cytology; D016022:Case-Control Studies; D002452:Cell Count; D003080:Cold Temperature; D004847:Epithelial Cells; D005260:Female; D006801:Humans; D007511:Ischemia; D008099:Liver / Q000098:blood supply; D016031:Liver Transplantation / Q000379:methods* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009926:Organ Preservation / Q000009:adverse effects / Q000379:methods*; D013997:Time Factors