Scavenging effect of Chinonin on NO and oxygen free radicals and its protective effect on the myocardium from the injury of ischemia-reperfusion.
The scavenging effect of Chinonin on NO and oxygen free radicals and its protective effect on myocardium from the ischemia-reperfusion injury was studied with electron spin resonance (ESR) and chemiluminescence techniques. Chinonin can effectively inhibit the oxidative activity of ONOO-, (the IC50 = 7 x 10 (-5) mmol/L) and scavenge oxygen free radicals generated from the reaction of xanthine and xanthine oxidase (the IC50 = 2/5 x 10(-4) mmol/l). It is difficult to find another antioxidant which can scavenge so effectively both ONOO- and oxygen free radicals simultaneously. In the system of ischemia-reperfusion injury of myocardium, Chinonin can, in parallel, scavenge the NO and oxygen free radicals generated from the ischemia-reperfused myocardium, and decrease the activities of lactate dehydrogenase (LDH) and creatine kinase (CK) in the coronary artery effluent of ischemia-reperfused heart and therefore protect the heart from ischemia-reperfusion injury. The protective effect of 0.1 mmol/l Chinonin is similar to that of 1500 U/ml SOD and catalase.
Zhao
B
B
Institute of Biophysics, Academia Sinica, Beijing, China.
Shen
J
J
Li
M
M
Li
M
M
Wan
Q
Q
Xin
W
W
D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives / Q000494:pharmacology; D002374:Catalase / Q000494:pharmacology; D004578:Electron Spin Resonance Spectroscopy; D004791:Enzyme Inhibitors / Q000494:pharmacology; D016166:Free Radical Scavengers / Q000494:pharmacology*; D005609:Free Radicals / Q000378:metabolism; D006027:Glycosides / Q000494:pharmacology*; D006321:Heart / Q000187:drug effects* / Q000502:physiology / Q000503:physiopathology; D006454:Hemoglobins / Q000378:metabolism; D066298:In Vitro Techniques; D007700:Kinetics; D008163:Luminescent Measurements; D008297:Male; D017202:Myocardial Ischemia / Q000378:metabolism / Q000517:prevention & control*; D015428:Myocardial Reperfusion Injury / Q000378:metabolism / Q000517:prevention & control*; D009206:Myocardium / Q000378:metabolism*; D019331:NG-Nitroarginine Methyl Ester; D009569:Nitric Oxide / Q000378:metabolism*; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors; D010084:Oxidation-Reduction; D051381:Rats; D017207:Rats, Sprague-Dawley; D014966:Xanthenes / Q000494:pharmacology*; D014969:Xanthine Oxidase / Q000378:metabolism; D044004:Xanthones
D004791:Enzyme Inhibitors; D016166:Free Radical Scavengers; D005609:Free Radicals; D006027:Glycosides; D006454:Hemoglobins; D014966:Xanthenes; D044004:Xanthones; C013592:mangiferin; D009569:Nitric Oxide; D001120:Arginine; D002374:Catalase; D019001:Nitric Oxide Synthase; D014969:Xanthine Oxidase; D019331:NG-Nitroarginine Methyl Ester