Upregulation of ICAM-1, IL-1 and reactive oxygen intermediates (ROI) by exogenous antigens from Plasmodium falciparum parasites in vitro, and of sICAM-1 in the acute phase of malaria.
After 4 hours of stimulation of human mononuclear leukocytes in the presence of 300 ng/ml exogenous Plasmodium falciparum antigens, the ICAM-1 expression increased variably from 15% to 375%. Simultaneously, an increase of IL-1 mRNA production could be observed in Northern blot hybridizations with a specific cDNA gene probe for human IL-1 alpha labelled with digoxigenin. Furthermore, the reactive oxygen intermediates (ROI) production was also found to be enhanced in similar conditions. Additionally, when the levels of soluble ICAM-1 (sICAM-1) in plasma of 122 patients with P. falciparum or Plasmodium vivax malaria were analyzed in an enzyme immunoassay (EIA), significant sICAM-1 increases were found, more pronounced in patients with P. falciparum malaria, in comparison with healthy controls and with the same patients 4 weeks after chemotherapy. The presented results indicate that the expression of ICAM-1 may also be upregulated by exogenous Plasmodium antigens besides cytokines like IL-1 during the acute phase of malaria, with subsequently elevated sICAM-1 concentrations in blood.
Prada
J
J
Landesinstitut für Tropenmedizin Berlin, Germany.
Graninger
W
W
Lehman
L G
LG
Metzger
W
W
Neifer
S
S
Zotter
G M
GM
Thalhammer
F
F
Bienzle
U
U
Kremsner
P G
PG
D000208:Acute Disease; D000818:Animals; D000953:Antigens, Protozoan / Q000502:physiology*; D002478:Cells, Cultured; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis*; D007375:Interleukin-1 / Q000096:biosynthesis*; D007963:Leukocytes, Mononuclear / Q000378:metabolism / Q000469:parasitology; D016778:Malaria, Falciparum / Q000188:drug therapy / Q000276:immunology / Q000378:metabolism*; D010963:Plasmodium falciparum / Q000276:immunology*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D012995:Solubility; D015854:Up-Regulation / Q000276:immunology
D000953:Antigens, Protozoan; D007375:Interleukin-1; D017382:Reactive Oxygen Species; D018799:Intercellular Adhesion Molecule-1