Simultaneous determination of catecholamines, their basic metabolites and serotonin in urine by high-performance liquid chromatography using a mixed-mode column and an eight-channel electrochemical detector.
A high-performance liquid chromatographic method for simultaneous determination of free catecholamines, their basic metabolites and serotonin in human urine was developed. The compounds were separated on a precolumn of cation-exchange resin and a mixed-mode (C18/cation-exchange) column and determined by an eight-channel electrochemical detector. By this method, free norepinephrine (NE), epinephrine (E), dopamine (DA), normetanephrine (NMN), metanephrine (MN), 3-methoxy-tyramine (3-MT) and serotonin (5-HT) were determined in 40 min with inter-assay precision of 1.9, 3.2, 1.2, 1.6, 1.6, 5.5 and 3.2%, respectively. Detection limits were between 0.05 (E) and 0.3 (3-MT) pmol. The mean values of analytical recovery for NE, E, DA, NMN, MN, 3-MT, 5-HT were 99, 104, 104, 99, 102, 109 and 99%, respectively.
Mashige
F
F
Department of Laboratory Medicine, Faculty of Medicine, University of Tokyo, Japan.
Matsushima
Y
Y
Miyata
C
C
Yamada
R
R
Kanazawa
H
H
Sakuma
I
I
Takai
N
N
Shinozuka
N
N
Ohkubo
A
A
Nakahara
K
K
D002021:Buffers; D002138:Calibration; D002395:Catecholamines / Q000378:metabolism / Q000652:urine*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004298:Dopamine / Q000031:analogs & derivatives / Q000652:urine; D004563:Electrochemistry; D004837:Epinephrine / Q000652:urine; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D008676:Metanephrine / Q000652:urine; D009638:Norepinephrine / Q000652:urine; D009647:Normetanephrine / Q000652:urine; D010084:Oxidation-Reduction; D012701:Serotonin / Q000652:urine*
D002021:Buffers; D002395:Catecholamines; D007202:Indicators and Reagents; D009647:Normetanephrine; D012701:Serotonin; D008676:Metanephrine; C001746:3-methoxytyramine; D004298:Dopamine; D009638:Norepinephrine; D004837:Epinephrine