title: Impaired negative selection of T cells in Hodgkin's disease antigen CD30-deficient mice.
abstract: CD30 is found on Reed-Sternberg cells of Hodgkin's disease and on a variety of non-Hodgkin's lymphoma cells and is up-regulated on cells after Epstein-Barr virus, human T cell leukemia virus, and HIV infections. We report here that the thymus in CD30-deficient mice contains elevated numbers of thymocytes. Activation-induced death of thymocytes after CD3 cross-linking is impaired both in vitro and in vivo. Breeding the CD30 mutation separately into alpha beta TCR-or gamma delta TCR-transgenic mice revealed a gross defect in negative but not positive selection. Thus, like TNF-receptors and Fas/Apo-1, the CD30 receptor is involved in cell death signaling. It is also an important coreceptor that participates in thymic deletion.
authors.affiliation: Amgen Institute, Department of Medical Biophysics, University of Toronto, Ontario, Canada.
authors.forename: R
authors.initials: R
authors.lastname: Amakawa
authors.forename: A
authors.initials: A
authors.lastname: Hakem
authors.forename: T M
authors.initials: TM
authors.lastname: Kundig
authors.forename: T
authors.initials: T
authors.lastname: Matsuyama
authors.forename: J J
authors.initials: JJ
authors.lastname: Simard
authors.forename: E
authors.initials: E
authors.lastname: Timms
authors.forename: A
authors.initials: A
authors.lastname: Wakeham
authors.forename: H W
authors.initials: HW
authors.lastname: Mittruecker
authors.forename: H
authors.initials: H
authors.lastname: Griesser
authors.forename: H
authors.initials: H
authors.lastname: Takimoto
authors.forename: R
authors.initials: R
authors.lastname: Schmits
authors.forename: A
authors.initials: A
authors.lastname: Shahinian
authors.forename: P
authors.initials: P
authors.lastname: Ohashi
authors.forename: J M
authors.initials: JM
authors.lastname: Penninger
authors.forename: T W
authors.initials: TW
authors.lastname: Mak
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology; D001483:Base Sequence; D016923:Cell Death / Q000276:immunology; D002454:Cell Differentiation / Q000235:genetics / Q000276:immunology; D002469:Cell Separation; D017353:Gene Deletion; D015870:Gene Expression / Q000276:immunology; D006689:Hodgkin Disease / Q000276:immunology; D017730:Ki-1 Antigen / Q000235:genetics / Q000276:immunology*; D008198:Lymph Nodes / Q000166:cytology; D008297:Male; D051379:Mice; D008817:Mice, Mutant Strains; D008969:Molecular Sequence Data; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics / Q000276:immunology; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000235:genetics / Q000276:immunology; D015398:Signal Transduction / Q000276:immunology; D018089:Superantigens / Q000276:immunology; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology* / Q000648:ultrastructure; D013950:Thymus Gland / Q000166:cytology / Q000473:pathology
chemical_list: D017730:Ki-1 Antigen; D016693:Receptors, Antigen, T-Cell, alpha-beta; D016692:Receptors, Antigen, T-Cell, gamma-delta; D018089:Superantigens