title: Detailed mapping and loss of heterozygosity analysis suggests a suppressor locus involved in sporadic breast cancer within a distal region of chromosome band 17p13.3.
abstract: The chromosome region 17p13.3 is thought to encode a tumour suppressor gene involved in sporadic breast cancer and other malignancies. Physical ordering of markers has been carried out by a series of multicolour fluorescent in situ hybridisation (FISH) experiments, using isolated yeast artificial chromosomes (YACs) and cosmids. Eight polymorphic markers ordered within this new physical map and one external marker were used to investigate the pattern of loss of heterozygosity in a panel of 40 sporadic breast tumour patients. The data revealed a region of high loss (60%) within distal 17p13.3, defined by markers D17S926, D17S695 and D17S849 which mapped close together. A contig of YACs was constructed physically linking these three markers.
authors.affiliation: CRC Department of Cancer Genetics, Paterson Institute for Cancer Research, Christie Hospital, Manchester, UK.
authors.forename: M
authors.initials: M
authors.lastname: Stack
authors.forename: D
authors.initials: D
authors.lastname: Jones
authors.forename: G
authors.initials: G
authors.lastname: White
authors.forename: D S
authors.initials: DS
authors.lastname: Liscia
authors.forename: T
authors.initials: T
authors.lastname: Venesio
authors.forename: G
authors.initials: G
authors.lastname: Casey
authors.forename: D
authors.initials: D
authors.lastname: Crichton
authors.forename: J
authors.initials: J
authors.lastname: Varley
authors.forename: E
authors.initials: E
authors.lastname: Mitchell
authors.forename: J
authors.initials: J
authors.lastname: Heighway
mesh_terms: D001483:Base Sequence; D001943:Breast Neoplasms / Q000235:genetics*; D002871:Chromosome Banding; D002872:Chromosome Deletion; D002874:Chromosome Mapping; D018244:Chromosomes, Artificial, Yeast; D002886:Chromosomes, Human, Pair 17; D005260:Female; D016147:Genes, Tumor Suppressor; D006579:Heterozygote; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data