title: [A gene expression study and the isolation and immunobiological properties of the iron-regulated membrane proteins of the plague microbe].
abstract: The study has examined how the plague bacillus expresses genes of two high-molecular weight (190 and 240 kD) iron-regulated membrane proteins (HMWP). The latter were purified and assayed for their immunobiological properties in vitro and in vivo. DNA-probing (at E.Carniel's Laboratory, Pasteur Institute, Paris) has indicated that Yersinia pestis strains having genes that code for HMWP synthesis circulate in the natural foci of plague which are situated in the former USSR. The use of HMWP in combination with live plague vaccine EV was found to greatly enhance its immunogenicity.
authors.forename: M Iu
authors.initials: MIu
authors.lastname: Ledvanov
authors.forename: E
authors.initials: E
authors.lastname: Carniel
authors.forename: S N
authors.initials: SN
authors.lastname: Tikhonov
authors.forename: Iu A
authors.initials: IuA
authors.lastname: Popov
authors.forename: A N
authors.initials: AN
authors.lastname: Kulichenko
authors.forename: M N
authors.initials: MN
authors.lastname: Kireev
authors.forename: S A
authors.initials: SA
authors.lastname: Korovkin
authors.forename: N Iu
authors.initials: NIu
authors.lastname: Stukova
authors.forename: E Iu
authors.initials: EIu
authors.lastname: Lotsmanova
authors.forename: V A
authors.initials: VA
authors.lastname: Anisimova
mesh_terms: D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000096:biosynthesis / Q000276:immunology / Q000302:isolation & purification*; D015342:DNA Probes; D004269:DNA, Bacterial / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D006168:Guinea Pigs; D007501:Iron / Q000378:metabolism*; D051379:Mice; D008970:Molecular Weight; D015010:Yersinia pestis / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D001425:Bacterial Outer Membrane Proteins; D015342:DNA Probes; D004269:DNA, Bacterial; D007501:Iron