The effect of fasting and fructose and glucose infusion on gluconeogenesis and triose phosphate flux in rats in vivo.
Gluconeogenesis was measured quantitatively by mass isotopomer distribution analysis in rats in vivo through determination of the isotopic enrichment of the pool of hepatic triose phosphates, the immediate precursors of glucose, using primarily [2-13C]glycerol infusion after fasting, and following fructose and glucose infusion. The results showed that the liver controls gluconeogenesis, the metabolic sources and disposal of triose phosphates, and the contribution of glucose from glycogen in response to alterations in substrate availability.
Wolf
G
G
Department of Nutritional Sciences, University of California, Berkeley 94720, USA.
D000818:Animals; D002247:Carbon Isotopes; D005215:Fasting / Q000502:physiology*; D005632:Fructose / Q000008:administration & dosage / Q000378:metabolism / Q000494:pharmacology*; D005943:Gluconeogenesis / Q000187:drug effects*; D005947:Glucose / Q000008:administration & dosage / Q000378:metabolism / Q000494:pharmacology*; D005990:Glycerol / Q000008:administration & dosage / Q000494:pharmacology; D007262:Infusions, Intravenous; D010710:Phosphates / Q000378:metabolism*; D051381:Rats
D002247:Carbon Isotopes; D010710:Phosphates; D005632:Fructose; D005947:Glucose; D005990:Glycerol