title: Amplification of complete insect mitochondrial genome in two easy pieces.
abstract: The entire insect mitochondrial genome has been amplified in just two PCR reactions. 'Universal' insect primers and techniques to optimize amplification of long PCR products were used to obtain segments of the mitochondrial genomes of Helicoverpa zea and Heliothis virescens (Lepidoptera) ranging in size from 3.7 kb to 14 kb or about 25% to over 85% of a typical 16 kb mtDNA. Overlapping pairs of fragments contain the complete mtDNA sequence.
authors.affiliation: United States Department of Agriculture, Agricultural Research Service, Fargo, North Dakota, USA.
authors.forename: R L
authors.initials: RL
authors.lastname: Roehrdanz
mesh_terms: D000818:Animals; D004272:DNA, Mitochondrial; D005784:Gene Amplification; D017344:Genes, Insect; D016678:Genome; D009036:Moths / Q000235:genetics; D016133:Polymerase Chain Reaction
chemical_list: D004272:DNA, Mitochondrial