The herpes simplex virus type 2 strain HG52 RL1 gene contains a 154 bp intron as predicted from sequence analysis.
The published sequence of the herpes simplex virus (HSV) type 2 strain HG52 neurovirulence gene RL1 indicated that, unlike the HSV-1 homologue, the gene contains a 154 bp intron. This intron contains six complete and one partial copy of a 19 bp repeat which encodes a stop codon; thus all three forward frames are blocked. By RT-PCR of poly(A)+ RNA, using primers on either side of the proposed intron, we have isolated a partial cDNA clone corresponding to the predicted spliced mRNA.
Harland
J E
JE
MRC Virology Unit, Institute of Virology, Glasgow, UK.
Bdour
S
S
Brown
S M
SM
MacLean
A R
AR
D001483:Base Sequence; D004279:DNA, Viral; D005091:Exons; D005814:Genes, Viral; D018258:Herpesvirus 2, Human / Q000235:genetics* / Q000472:pathogenicity; D007438:Introns; D014764:Viral Proteins / Q000235:genetics*; D014774:Virulence / Q000235:genetics
D004279:DNA, Viral; D014764:Viral Proteins; C066469:gamma 34.5 protein, Human herpesvirus 1