title: Effects of benzylpenicillin on glucose utilization, macromolecule synthesis and cell wall proteins of Listeria monocytogenes.
abstract: When growing cells were incubated in the presence of 100 ppm benzylpenicillin (BP) for 30 min and then harvested for metabolic studies, the initial rate of glucose utilization by resting cells was reduced by 19.6% compared to control cells, whereas the rates of protein, DNA and RNA synthesis were reduced by 40.7%, 55.0% and 87.5% respectively. Growth in the presence of 100 ppm BP for 2 h was found to result in a marked depletion of cell wall proteins.
authors.affiliation: Department of Food Science, University of Massachusetts, Amherst, USA.
authors.forename: L J
authors.initials: LJ
authors.lastname: Chen
authors.forename: J
authors.initials: J
authors.lastname: Wang
authors.forename: R E
authors.initials: RE
authors.lastname: Levin
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism; D002473:Cell Wall / Q000187:drug effects / Q000378:metabolism; D004269:DNA, Bacterial / Q000096:biosynthesis; D005947:Glucose / Q000378:metabolism; D008089:Listeria monocytogenes / Q000187:drug effects* / Q000378:metabolism; D046911:Macromolecular Substances; D010400:Penicillin G / Q000494:pharmacology*; D010406:Penicillins / Q000494:pharmacology*; D012329:RNA, Bacterial / Q000096:biosynthesis
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D046911:Macromolecular Substances; D010406:Penicillins; D012329:RNA, Bacterial; D005947:Glucose; D010400:Penicillin G