title: Developmental maturation of pineal gland function: synchronized CREM inducibility and adrenergic stimulation.
abstract: The cAMP response element modulator (CREM) gene encodes multiple activators and repressors of cAMP-responsive transcription. Differential splicing generates a developmental switch in CREM function during spermatogenesis, while the use of an alternative promoter is responsible for the production of a cAMP-inducible transcriptional repressor, ICER (inducible cAMP early repressor). The ICER promoter is strongly inducible by cAMP because of the presence of four tandemly repeated cAMP response elements. Furthermore, ICER negatively autoregulates the ICER promoter activity, thus generating a feedback loop. CREM constitutes an early response gene of the cAMP pathway in several neuroendocrine cells. We have previously shown that CREM is highly expressed in the adult rat pineal gland at nighttime. Here, we show that the only additional site of rhythmic ICER expression within the photoneuroendocrine system is the lamina intercalaris. Ontogenetically, the ICER day-night switch and cAMP inducibility mature in the pineal gland at the end of the first postnatal week. Importantly, this correlates with the onset of melatonin synthesis and the establishment of functional adrenergic innervation. At this developmental phase we document a significant increase in protein kinase A levels, thus suggesting that ICER inducibility reflects a complete maturation of the cAMP-dependent signaling pathway at the nuclear level.
authors.affiliation: Neurobiologie des Fonctions Rythmiques et Saisonnieres, Centre Nationale de Recherche Scientifique, Strasbourg, France.
authors.forename: J H
authors.initials: JH
authors.lastname: Stehle
authors.forename: N S
authors.initials: NS
authors.lastname: Foulkes
authors.forename: P
authors.initials: P
authors.lastname: Pévet
authors.forename: P
authors.initials: P
authors.lastname: Sassone-Corsi
mesh_terms: D000818:Animals; D002940:Circadian Rhythm / Q000502:physiology*; D000242:Cyclic AMP / Q000502:physiology*; D051786:Cyclic AMP Response Element Modulator; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D005246:Feedback; D018507:Gene Expression Regulation, Developmental; D017403:In Situ Hybridization; D008297:Male; D010870:Pineal Gland / Q000196:embryology / Q000254:growth & development*; D011401:Promoter Regions, Genetic; D051381:Rats; D017208:Rats, Wistar; D012097:Repressor Proteins; D012160:Retina / Q000196:embryology / Q000502:physiology; D013493:Suprachiasmatic Nucleus / Q000196:embryology / Q000502:physiology
chemical_list: D004268:DNA-Binding Proteins; D012097:Repressor Proteins; D051786:Cyclic AMP Response Element Modulator; D000242:Cyclic AMP