Flow cytometric measurement of DNA S-phase in human bone marrow cells: correcting for peripheral blood contamination.
The relationship between bone marrow (BM) cells with S-phase DNA content and the amount of peripheral blood contamination estimated as percentage lymphocytes+monocytes (L+MO) present in BM samples has been investigated in a total of 136 BM aspirates and biopsy expellates from 35 hematologically healthy individuals. A significant negative correlation was demonstrated between total, erythroid and myeloid BM cells in S-phase and the percentage of L+MO in the aspirates (r=0.84, 0.57 and 0.49, respectively; p<0.0001). Based on the equation of the slope of the regression line, a correction formula adjusting the measured value of BM cells in S-phase to varying amounts of L+MO percentage has been worked out for the total and erythroid BM cells. In contrast, highly proliferating myelomonocytic cells and CD34+ cells did not show any significant correlation between cells in S-phase and percentage L+MO, indicating that peripheral blood contamination of BM aspirates estimates the degree of peripheral blood contamination, as well as make possible a correct estimation of the DNA synthesis of several BM populations. The method is especially applicable when frequent BM sampling is required.
Abrahamsen
J F
JF
Department of Pathology, Haukeland Hospital, University of Bergen, Norway.
Smaaland
R
R
Skjaerven
R
R
Laerum
O D
OD
D000328:Adult; D000465:Algorithms; D016477:Artifacts; D001706:Biopsy; D001769:Blood; D001853:Bone Marrow / Q000737:chemistry; D001854:Bone Marrow Cells; D001856:Bone Marrow Examination / Q000379:methods*; D004247:DNA / Q000032:analysis*; D004906:Erythrocyte Count; D005434:Flow Cytometry; D006412:Hematopoietic Stem Cells / Q000737:chemistry / Q000166:cytology; D006801:Humans; D018189:Immunomagnetic Separation; D007958:Leukocyte Count; D018655:Lymphocyte Count; D008297:Male; D008875:Middle Aged; D009000:Monocytes; D012016:Reference Values; D016196:S Phase
D004247:DNA