Sequence of the rat PB-inducible CYP2B1 promoter.
The 5' flanking region of the rat PB-inducible CYP2B1 gene was isolated and the sequence from +27 to -3878 was determined. This sequence contains several putative binding sites for the liver-enriched factors HNF3 as well as an AP1, two NF-kappa B and several possible STAT sites. The promoter sequence of the CYP2B1 gene was compared to that of the CYP2B2 sequence, published by Hoffman et al. ((1992) Gene Exp. 2, 353-362) and was found to be almost identical up to -2300 bp, beyond which it diverges significantly from the remaining published sequence of CYP2b2 gene. Transient transfection experiments in the differentiated hepatoma cell line, FGC4, showed that the 3.9 kb promoter was expressed, however, an increase in reporter activity was not observed in the presence of phenobarbital.
Shaw
P M
PM
Department of Cell Biology, New York University School of Medicine, New York, NY 10016, USA.
Edigkaufer
M
M
Doehmer
J
J
Adesnik
M
M
D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D002460:Cell Line; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D004247:DNA / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D008114:Liver Neoplasms, Experimental / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D010634:Phenobarbital / Q000494:pharmacology; D011401:Promoter Regions, Genetic; D051381:Rats; D012689:Sequence Homology, Nucleic Acid; D013250:Steroid Hydroxylases / Q000235:genetics*; D014157:Transcription Factors / Q000378:metabolism; D014162:Transfection
D014157:Transcription Factors; D004247:DNA; D003577:Cytochrome P-450 Enzyme System; D013250:Steroid Hydroxylases; D001189:Aryl Hydrocarbon Hydroxylases; C025619:steroid 16-beta-hydroxylase; D010634:Phenobarbital