[Proliferation behavior of cholesteatoma].
Two types of growth can be observed clinically in cholesteatoma: an aggressive and a less aggressive type. In this study monoclonal antibodies to Ki-67 proliferation antigen, epidermal growth-related factor (EGFR) and collagen type IV were used to examine cholesteatoma specimens from 36 patients undergoing tympanomastoid surgery. Mitotic cells were found in various amounts in each sample and an increase was seen in tissue folds and recesses. No difference was observed between the aggressive and less aggressive types. The expression of EGFR was restricted to basal and suprabasal layers and showed no differences between the groups. Small defects of the basal membrane were seen frequently and reflected the invasive growth potential of the cholesteatoma epithelium. These results showed that clinically less aggressive cholesteatomas also have a high proliferation rate. As a consequence, leaving small rests of epithelium in situ during middle ear surgery increases the risk for recurrences. Morphologically, no differences could be observed between an aggressive and a less aggressive cholesteatoma.
Hoppe
F
F
Universitäts-HNO-Klinik Würzburg.
D001485:Basement Membrane / Q000473:pathology; D002455:Cell Division / Q000502:physiology*; D018424:Cholesteatoma, Middle Ear / Q000473:pathology* / Q000601:surgery; D003094:Collagen / Q000032:analysis; D004432:Ear, Middle / Q000473:pathology / Q000601:surgery; D066246:ErbB Receptors / Q000032:analysis; D006133:Growth Substances / Q000032:analysis*; D006801:Humans; D007124:Immunoenzyme Techniques; D019394:Ki-67 Antigen; D008940:Mitotic Index; D009363:Neoplasm Proteins / Q000032:analysis; D009687:Nuclear Proteins / Q000032:analysis
D006133:Growth Substances; D019394:Ki-67 Antigen; D009363:Neoplasm Proteins; D009687:Nuclear Proteins; D003094:Collagen; D066246:ErbB Receptors