Energetics and product formation by Saccharomyces cerevisiae grown in anaerobic chemostats under nitrogen limitation.
Anaerobic fermentation of glucose (20 g/l) by Saccharomyces cerevisiae CBS 8066 was studied in a chemostat (dilution rate = 0.05-0.25 h-1) at different concentrations of the nitrogen source (5.00 g/l or 0.36 g/l ammonium sulphate). The ethanol yield (g ethanol produced/g glucose consumed) was found to be higher and the glycerol yield (g glycerol formed/g glucose consumed) lower during nitrogen limitation than under carbon limitation. The biomass yield on ATP (g dry weight biomass produced/mol ATP consumed), was consequently found to be lower during nitrogen-limited conditions.
Lidén
G
G
Department of Chemical Reaction Engineering, Chalmers University of Technology, Göteborg, Sweden.
Persson
A
A
Gustafsson
L
L
Niklasson
C
C
D000255:Adenosine Triphosphate / Q000378:metabolism; D000693:Anaerobiosis; D018533:Biomass; D002244:Carbon / Q000378:metabolism; D004734:Energy Metabolism; D005285:Fermentation; D005947:Glucose / Q000378:metabolism*; D005990:Glycerol / Q000378:metabolism*; D009172:Mycology / Q000295:instrumentation*; D009584:Nitrogen / Q000378:metabolism*; D000644:Quaternary Ammonium Compounds / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism*
D000644:Quaternary Ammonium Compounds; D002244:Carbon; D000255:Adenosine Triphosphate; D005947:Glucose; D009584:Nitrogen; D005990:Glycerol