title: [Conformational state of oncoprecipitin cyprein and its immunologic activity].
abstract: The effect of solution ionic strength, calcium ion concentration, and temperature on spatial structure of cyprein was examined by CD, UV, and fluorescence spectroscopy. The secondary structure of the cyprein molecule was calculated from CD spectra, and the prevalence of the beta-structure (85%) was shown. An irreversible conformational transition in the range 55-60 degrees C was found, which reduces the binding activity of cyprein in interaction with carcinoembryonic antigen (CEA) and anti-cyprein antibodies. In the latter case, the binding activity was reversibly restored. Cyprein was shown to be a calcium-binding protein. Binding of calcium by cyprein and increasing the ionic strength of solution affect only tertiary structure of the protein. At an ionic strength of solution close to physiological conditions, calcium-bound cyprein shows maximum binding to CEA and anti-cyprein antibodies. It was shown by difference UV spectroscopy that cyprein does not interact specifically with the monosaccharides of the carbohydrate chains of CEA: fucose, mannose, galactose, and N-acetylglucosamine.
authors.forename: V P
authors.initials: VP
authors.lastname: Glazunov
authors.forename: Z A
authors.initials: ZA
authors.lastname: Tarankova
authors.forename: S E
authors.initials: SE
authors.lastname: Odinokov
authors.forename: A F
authors.initials: AF
authors.lastname: Pavlenko
authors.forename: I V
authors.initials: IV
authors.lastname: Chikalovets
authors.forename: A V
authors.initials: AV
authors.lastname: Kurika
mesh_terms: D002118:Calcium / Q000378:metabolism; D002135:Calcium-Binding Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D002272:Carcinoembryonic Antigen / Q000276:immunology; D002942:Circular Dichroism; D006358:Hot Temperature; D009005:Monosaccharides / Q000737:chemistry; D009994:Osmolar Concentration; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
chemical_list: D002135:Calcium-Binding Proteins; D002272:Carcinoembryonic Antigen; D009005:Monosaccharides; D002118:Calcium