[Effects of berberine on single Ca2+ channel current in cultured embryonic chick ventricular myocytes].
On cultured embryonic chick ventricular myocytes, we investigated the influence of berberine (BR) on single Ca2+ channel current using patch clamp technique with cell-attached configuration. The results showed that BR could increase the single Ca2+ channel current significantly and the increases of open availability (Ps) and open probability (Po) caused by BR were 114.2% (P < 0.001) and 75.3% (P < 0.05), respectively. The beta-receptor antagonist propranolol and Ca2+ channel blocker ispotin could reverse the effects of BR on single Ca2+ channel, suggesting that (1) BR works as a Ca2+ channel agonist; (2) the regulating effects of BR on single Ca2+ channel is depending upon the phosphorylation process induced by beta-receptor activity.
Zhou
Z
Z
Lan
T
T
Li
H
H
Zhang
Y
Y
Wang
Y
Y
D000319:Adrenergic beta-Antagonists / Q000494:pharmacology; D000818:Animals; D001599:Berberine / Q000494:pharmacology*; D002120:Calcium Channel Agonists / Q000494:pharmacology*; D002121:Calcium Channel Blockers / Q000494:pharmacology; D015220:Calcium Channels / Q000187:drug effects*; D002462:Cell Membrane; D002478:Cells, Cultured; D002642:Chick Embryo; D009206:Myocardium / Q000166:cytology*; D011433:Propranolol / Q000494:pharmacology; D014700:Verapamil / Q000494:pharmacology
D000319:Adrenergic beta-Antagonists; D002120:Calcium Channel Agonists; D002121:Calcium Channel Blockers; D015220:Calcium Channels; D001599:Berberine; D011433:Propranolol; D014700:Verapamil