Pharmacological activity of chemically modified subfragment from human serum IgG. XIV. Inhibitory effect of carboxamide-methylated light chain (G1L) on tyrosine phosphorylation and tumor necrosis factor-alpha production from murine macrophages stimulated by lipopolysaccharide.
Carboxamide-methylated light chain (G1L) from human serum IgG inhibited the secretion of tumor necrosis factor (TNF-alpha), one of the inflammatory cytokines, from adherent splenocytes and thioglycolate-induced peritoneal macrophages. The inhibition of TNF-alpha secretion by G1L was associated with disappearance of tyrosine phosphorylation on about 40 kDa protein when thioglycolate-induced peritoneal macrophages were stimulated with lipopolysaccharide (LPS). It is possible that this G1L anti-inflammatory activity occurs through the blockage of the phosphorylation of about 40 kDa protein.
Mimura
T
T
Division of Bio-Medical and Immunological Chemistry, Faculty of Pharmaceutical Sciences, Osaka University, Japan.
Takeuchi
K
K
Hiramatsu
K
K
Tsujikawa
K
K
Kohama
Y
Y
Itoh
S
S
D000818:Animals; D015153:Blotting, Western; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004731:Endotoxins / Q000494:pharmacology; D006801:Humans; D007074:Immunoglobulin G / Q000737:chemistry / Q000494:pharmacology*; D007147:Immunoglobulin Light Chains / Q000737:chemistry / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008745:Methylation; D051379:Mice; D008813:Mice, Inbred ICR; D010766:Phosphorylation; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism; D013154:Spleen / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D013601:T-Lymphocytes / Q000187:drug effects / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*; D014443:Tyrosine / Q000378:metabolism*
D004731:Endotoxins; D007074:Immunoglobulin G; D007147:Immunoglobulin Light Chains; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D014443:Tyrosine; C044101:endotoxin, Escherichia coli; D011505:Protein-Tyrosine Kinases