Effect of melittin on electrophysiological parameters in the frog cornea epithelium.
The effect of melittin on electrophysiological parameters of the bullfrog cornea was studied using an in vitro preparation. Epithelial cells of corneas were impaled with microelectrodes. Experiments were done under short-circuit current (Isc) conditions. Melittin was added in concentrations of 10(-5)M to the stromal or 10(-6)M to the tear solution. The effects of melittin were as follows: (i) stromal side, a decrease in Isc; an increase in the apical membrane fractional resistance, fRo; no change in the transepithelial conductance, gt; and a depolarization of the intracellular potential, Vo; (ii) tear side, an initial (first 10 min) increase and then a decrease in Isc; a decrease in fRo; an initial (first 10 min) increase with subsequent small decrease in gt; and a depolarization of Vo. Changes in tear Na+, but not in tear K+, with melittin present in tear solution, induced changes in some electrical parameters. The effects on the tear side may be explained by opening of nonspecific channels in the apical membrane with some specificity for Na+ channel. The subsequent effects on the tear and the effects on the stromal side may be explained by an inhibition of the primary transport system, that is, of the Na+/K+ -ATPase pump located in the basolateral membrane. In these experiments, there was no evidence of opening of channels in the basolateral membrane.
Carrasquer
G
G
Department of Medicine (Nephrology), University of Louisville, Kentucky 40292, USA.
Yang
S
S
Schwartz
M
M
Dinno
M A
MA
D000818:Animals; D003315:Cornea / Q000187:drug effects* / Q000502:physiology; D004848:Epithelium / Q000187:drug effects / Q000502:physiology; D007473:Ion Channels / Q000187:drug effects; D008555:Melitten / Q000494:pharmacology*; D011892:Rana catesbeiana; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors
D007473:Ion Channels; D008555:Melitten; D000254:Sodium-Potassium-Exchanging ATPase