title: Demonstration of infectious salmon anaemia (ISA) viral antigens in cell cultures and tissue sections.
abstract: Rabbit polyclonal antibodies and mouse monoclonal antibodies (MAb) directed against infectious salmon anaemia virus (ISAV) were produced using a virus prepared in a newly established cell line culture developed from Atlantic salmon head kidney (SHK-1) cells. These antibodies were used to establish an indirect fluorescent antibody test for the detection of viral antigens in cell cultures and tissue cryosections. Specific fluorescence was detected in ISAV-infected cell cultures using rabbit polyclonal and MAb. One selected MAb produced specific staining for ISAV in the tissue sections from all the examined organs of ISAV-infected Atlantic salmon 20 d post infection. Fluorescence was mainly found in the endothelial cells and in single cells scattered throughout the parenchyma of the organs.
authors.affiliation: Department of Virology, Central Veterinary Laboratory, Oslo, Norway.
authors.forename: K
authors.initials: K
authors.lastname: Falk
authors.forename: B H
authors.initials: BH
authors.lastname: Dannevig
mesh_terms: D000740:Anemia / Q000175:diagnosis / Q000473:pathology / Q000662:veterinary*; D000818:Animals; D000906:Antibodies; D000911:Antibodies, Monoclonal; D000956:Antigens, Viral / Q000032:analysis*; D002460:Cell Line; D004730:Endothelium, Vascular / Q000473:pathology / Q000821:virology; D005393:Fish Diseases; D019084:Fluorescent Antibody Technique, Indirect; D006321:Heart / Q000821:virology; D007668:Kidney; D016180:Lentivirus Infections / Q000175:diagnosis / Q000473:pathology / Q000662:veterinary*; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology; D009206:Myocardium / Q000473:pathology; D011817:Rabbits / Q000276:immunology; D012474:Salmon / Q000821:virology*
chemical_list: D000906:Antibodies; D000911:Antibodies, Monoclonal; D000956:Antigens, Viral