Comparison of axonemal proteins from two kinds of Tetrahymena. I. Different characteristics of dyneins in heat stability.
Tetrahymena thermophila could still swim after incubation of the cell body at 40 degrees C for 30 min, whereas Tetrahymena pyriformis did not show any motility after the treatment. Turbidity measurements revealed that axonemes of T. pyriformis lost ATP-dependent sliding activity by the heat treatment, whereas those of T. thermophilia still had the activity under the same conditions. In connection with this difference in susceptibility to high temperature, the biochemical characteristics of dyneins were compared between the two species of Tetrahymena. Axonemal dyneins from the two species had significant vanadate-sensitive ATPase activity even after the heat treatment. Native gel electrophoresis and the following two-dimensional electrophoresis showed that the outer arm dynein of T. thermophilia is more stable in maintaining native configuration than that of T. pyriformis against the heat treatment, although both treated dyneins keep three (alpha, beta and gamma) subunits. Analysis by peptide mapping demonstrated that beta- and gamma-subunits of the outer arm dynein are considerably different in amino acid sequences between the two species. These results imply that dynein of T. thermophilia changed their amino acid sequences and biochemical characteristics to adapt to high temperature.
Takaya
C
C
Department of Life Sciences, Hiroshima Women's University, Japan.
Kosaka
A
A
Kohno
K
K
Kusano
T
T
Nakamura
K
K
D000595:Amino Acid Sequence; D000818:Animals; D002923:Cilia / Q000737:chemistry* / Q000648:ultrastructure; D004398:Dyneins / Q000737:chemistry*; D004795:Enzyme Stability; D006358:Hot Temperature; D008970:Molecular Weight; D009391:Nephelometry and Turbidimetry; D010449:Peptide Mapping; D015800:Protozoan Proteins / Q000737:chemistry*; D013045:Species Specificity; D013769:Tetrahymena pyriformis / Q000737:chemistry* / Q000648:ultrastructure; D016808:Tetrahymena thermophila / Q000737:chemistry* / Q000648:ultrastructure
D015800:Protozoan Proteins; D004398:Dyneins