title: Microinjection of fluorescence dye in a plant cell and its intercellular translocation using a multi-channel microelectrode system.
abstract: Lucifer yellow CH dye (LY) was electrophoretically injected into a cell of a cultured tobacco (BY-2) using a multi-channel microelectrode (MME). Pulsing electricx potential (pulse width:90 ms, pulse interval: 80 ms) was applied between the potential-applying (PA) electrodes of both MMESs . The PA-electrode positioned in the LY-bearing cell was set negative against the other PA-electrode. When the pulse height was 340 V, intercellular translocation of LY from the LY-bearing cell to the neighboring cell was observed within 10 s.
authors.affiliation: Department of Biotechnology, Tokyo University of Agriculture and Technology, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Saito
authors.forename: A
authors.initials: A
authors.lastname: Ohi
authors.forename: H
authors.initials: H
authors.lastname: Matsuoka
mesh_terms: D002478:Cells, Cultured; D005456:Fluorescent Dyes / Q000737:chemistry*; D007546:Isoquinolines / Q000737:chemistry; D008839:Microelectrodes; D010944:Plants / Q000737:chemistry*; D015398:Signal Transduction
chemical_list: D005456:Fluorescent Dyes; D007546:Isoquinolines; C017475:lucifer yellow