Simple protocols for typing forensic biological evidence: chemiluminescent detection for human DNA quantitation and restriction fragment length polymorphism (RFLP) analyses and manual typing of polymerase chain reaction (PCR) amplified polymorphisms.
Methods for identity testing are described that enable extraction of DNA from biological samples, determination of the quantity of human DNA, and genetic analyses of the materials using restriction fragment length polymorphism (RFLP) typing and/or amplified fragment length polymorphism (AMP-FLP) typing of PCR products. The salient features of the procedures are simplicity, manual typing, nonradioactive chemiluminescent assays or silver staining for detection, and low cost. Most application-oriented laboratories involved in forensic and/or paternity testing should be able to implement these procedures.
Budowle
B
B
Forensic Science Research and Training Center, FBC Laboratory, FBI Academy, Quantico, VA, USA.
Baechtel
F S
FS
Comey
C T
CT
Giusti
A M
AM
Klevan
L
L
D001483:Base Sequence; D002874:Chromosome Mapping; D004247:DNA / Q000032:analysis* / Q000097:blood; D005554:Forensic Medicine / Q000379:methods*; D006801:Humans; D015151:Immunoblotting; D008163:Luminescent Measurements; D008297:Male; D018598:Minisatellite Repeats; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012091:Repetitive Sequences, Nucleic Acid; D012661:Semen / Q000737:chemistry
D004247:DNA