Small bowel preservation using a cavitary two-layer (University of Wisconsin solution/perfluorochemical) cold storage method.
Preservation of the small bowel by a cavitary two-layer (University of Wisconsin solution [UW]/perfluorochemical) cold storage method was evaluated in the heterotopic rat segmental small bowel transplant model. Simple cold storage UW was effective only for 24-hr preservation. However, the cavitary two-layer method using UW made it possible to prolong preservation time up to 48 hr. Furthermore, without oxygen bubbling, the cavitary two-layer method was not effective for 48-hr preservation. Histologic studies of grafts preserved by the cavitary two-layer method for 48 hr at 7 days after transplantation showed normal architecture of the intestinal mucosa. This study demonstrates that the oxygenation of the small bowel during preservation by the cavitary two-layer method using UW makes it possible to extend preservation time up to 48 hr in the heterotopic rat segmental small bowel transplant model.
Kuroda
Y
Y
First Department of Surgery, Kobe University School of Medicine, Japan.
Sakai
T
T
Suzuki
Y
Y
Tanioka
Y
Y
Matsumoto
S
S
Kim
Y
Y
Fujita
H
H
Hamano
M
M
Hasegawa
Y
Y
Ku
Y
Y
Saitoh
Y
Y
D000241:Adenosine; D000493:Allopurinol; D000818:Animals; D003080:Cold Temperature; D005069:Evaluation Studies as Topic; D005466:Fluorocarbons; D005978:Glutathione; D007328:Insulin; D007413:Intestinal Mucosa / Q000033:anatomy & histology; D007421:Intestine, Small / Q000033:anatomy & histology / Q000637:transplantation; D008297:Male; D009926:Organ Preservation / Q000295:instrumentation / Q000379:methods*; D019188:Organ Preservation Solutions; D010100:Oxygen; D011887:Raffinose; D051381:Rats; D011917:Rats, Inbred Lew; D013997:Time Factors; D014185:Transplantation, Isogeneic
D005466:Fluorocarbons; D007328:Insulin; D019188:Organ Preservation Solutions; C055206:University of Wisconsin-lactobionate solution; D000493:Allopurinol; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose; D010100:Oxygen