Detection of a high molecular weight heparin binding acidic growth factor in human placenta.
A heparin binding fibroblast growth factor with a molecular weight of approximately 45 kDa has been detected in human placenta. The protein was extracted from placenta and purified by a combination of ammonium sulfate precipitation, DE-52 anion exchange chromatography, G100 gel exclusion and heparin sepharose affinity chromatography. The molecule was shown to be monomeric after treatment with beta-mercaptoethanol, and had an acidic isoelectric point. The properties of the polypeptide described in this paper (45 kDa, Pi 4-5, monomeric, heparin binding fibroblast growth factor), suggest that it may represent an as yet undescribed growth factor.
Triantos
A
A
Department of Biological Chemistry, Medical School, Aristotelian University of Thessaloniki.
Koliakos
G
G
Kavoukopoulos
E
E
Dimitriadou
A
A
Trakatellis
A
A
D005260:Female; D005346:Fibroblast Growth Factors / Q000302:isolation & purification* / Q000378:metabolism; D006493:Heparin / Q000378:metabolism*; D006801:Humans; D008970:Molecular Weight; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D011485:Protein Binding
D005346:Fibroblast Growth Factors; D006493:Heparin