Physical properties of oxidized lipoproteins.
Oxidation of four major plasma lipoproteins (chylomicrons, very low, low and high density lipoproteins) induced by Cu2+ was found to increase their mobility during agarose gel electrophoresis, their absorption between 220 and 280 nm and fluorescence at 360/430 nm. Oxidation decreased lipoprotein absorption between 400 and 500 nm, fluorescence at 290/330 nm and fluidity of lipoprotein lipids. Changing physical properties of the lipoproteins indicated that both their lipid and protein moieties were affected by oxidation. It is suggested that the relative contribution of lipid vs. protein oxidation to the oxidation of whole lipoproteins reflects their lipid/protein ratio, decreasing from chylomicrons to high density lipoproteins.
Kontush
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Medical Clinic, University of Hamburg, Germany.
Kohlschütter
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Beisiegel
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D003300:Copper; D006801:Humans; D008074:Lipoproteins / Q000737:chemistry*; D010084:Oxidation-Reduction
D008074:Lipoproteins; D003300:Copper