title: Human hepatitis B virus and hepatocellular carcinoma. I. Experimental infection of tree shrews with hepatitis B virus.
abstract: Tree shrews (Tupaia belangeri chinenesis) can be experimentally infected with human hepatitis B virus (HBV) by inoculation with human serum positive for HBV, the experimental infection rate being 55.21%. Successive infections have been passed through five generations among the tree shrews inoculated with HBV-positive sera from the infected animals, the average infection rate being 94.0%. The experimental infection of tree shrews with HBV may be prevented by immunization with hepatitis B vaccine, the protection rate being 88.89%. Standard serum containing HBV at 10(8) CID (chimpanzee infection dose)/ml, was diluted 10(-6), 10(-7), 10(-8), 10(-9), and 10(-10) and produced infection rates of 80.0%, 88.8%, 66.7%, 55.6% and 42.9% respectively. Thus the CID50 in tree shrews may reach a dilution of 10(-9), which shows that tree shrews are sensitive to HBV infection. These results successfully establish tree shrews as a reliable and useful animal model for research on HBV infection and its relation to hepatocarcinogenesis.
authors.affiliation: Department of Pathology, Guangxi Institute for Research on Cancer, Nanning, P. R. China.
authors.forename: R Q
authors.initials: RQ
authors.lastname: Yan
authors.forename: J J
authors.initials: JJ
authors.lastname: Su
authors.forename: D R
authors.initials: DR
authors.lastname: Huang
authors.forename: Y C
authors.initials: YC
authors.lastname: Gan
authors.forename: C
authors.initials: C
authors.lastname: Yang
authors.forename: G H
authors.initials: GH
authors.lastname: Huang
mesh_terms: D000818:Animals; D004279:DNA, Viral / Q000032:analysis; D004195:Disease Models, Animal; D006509:Hepatitis B / Q000097:blood / Q000209:etiology* / Q000821:virology; D006514:Hepatitis B Surface Antigens / Q000032:analysis; D006801:Humans; D008113:Liver Neoplasms / Q000209:etiology*; D014417:Tupaia
chemical_list: D004279:DNA, Viral; D006514:Hepatitis B Surface Antigens