Method for immobilizing microbial cells on gel surface for dynamic AFM studies.
The processes of cell growth and budding of the yeast cells Saccharomyces cerevisiae, which were gently immobilized on 3% agar and submerged in culture medium, were successfully imaged with an atomic force microscope for 6-7 h. Similar experiments on chemically fixed cells did not detect any appreciable change in their appearance except in a few scannings at the very beginning, indicating that the dissolution of agar and/or scraping of its surface by the scanning tip, if any, did not significantly interfere with the images taken thereafter. The increment in the height of many of the untreated cells, accompanied by their lateral enlargement, was taken as an indication of successful imaging of the growth process of yeast cells, together with an image of a growing daughter cell attached to its mother cell.
Gad
M
M
Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Japan.
Ikai
A
A
D000362:Agar; D005782:Gels; D007700:Kinetics; D018625:Microscopy, Atomic Force / Q000379:methods*; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000254:growth & development*; D013499:Surface Properties; D013997:Time Factors
D005782:Gels; D000362:Agar