Gene expression of endothelin isoforms and receptors in endothelin-1 knockout mice.
Gene expression of endothelin (ET) isoforms and receptors was examined in ET-1 knockout mice and compared with that of wild-type mice. ET-1 mRNA and peptide levels were in parallel with the number of Edn1 authentic alleles. ET-2 mRNA levels in intestine and ET-3 mRNA and/or peptide levels in lung, brain, and intestine were not different among Edn1+/+ wild-type, Edn1+/- heterozygous, and Edn1-/- homozygous mice. ET(A) and ETB receptor mRNA levels in several organs also were not different among Edn1+/+ wild-type, Edn1+/- heterozygous, and Edn1-/- homozygous mice. These results suggest that gene expression of ET-2, ET-3, and ET(A/B) receptors is not affected by levels of ET-1 production and is regulated independently. Neither compensation by redundant expression of other ET isoforms nor upregulation of ET receptors could explain the elevated blood pressure in Edn1+/- heterozygous mice.
Maemura
K
K
Third Department of Internal Medicine, University of Tokyo, Japan.
Kurihara
H
H
Kurihara
Y
Y
Kuwaki
T
T
Kumada
M
M
Yazaki
Y
Y
D000818:Animals; D016232:Endothelins / Q000235:genetics* / Q000502:physiology; D015870:Gene Expression; D006973:Hypertension / Q000209:etiology; D051379:Mice; D008813:Mice, Inbred ICR; D018345:Mice, Knockout; D012333:RNA, Messenger / Q000032:analysis*; D017466:Receptors, Endothelin / Q000235:genetics*
D016232:Endothelins; D012333:RNA, Messenger; D017466:Receptors, Endothelin