title: sn-glycerol-1-phosphate dehydrogenase in Methanobacterium thermoautotrophicum: key enzyme in biosynthesis of the enantiomeric glycerophosphate backbone of ether phospholipids of archaebacteria.
abstract: One of the most characteristic features of archaebacterial ether phospholipids is the enantiomeric configuration of their glycerophosphate backbone (sn-glycerol-1-phosphate), that is the mirror image of the structure of the eubacterial or eukaryotic counterpart. The enzyme that forms glycerophosphate of this configuration was found for the first time in a cell-free extract of the methanogen, Methanobacterium thermoautotrophicum, and was identified as sn-glycerol-1-phosphate:NAD+ oxidoreductase (sn-glycerol-1-phosphate dehydrogenase) after partial purification. Because sn-glycerol-1-phosphate has been found to be a precursor of ether lipids of this organism, sn-glycerol-1-phosphate dehydrogenase is a key enzyme in the biosynthesis of the enantiomeric ether lipids of methanogens.
authors.affiliation: Department of Chemistry, University of Occupational and Environmental Health, Kitakyushu.
authors.forename: M
authors.initials: M
authors.lastname: Nishihara
authors.forename: Y
authors.initials: Y
authors.lastname: Koga
mesh_terms: D000818:Animals; D001105:Archaea / Q000737:chemistry / Q000378:metabolism; D005591:Chemical Fractionation; D002845:Chromatography; D004099:Dihydroxyacetone Phosphate / Q000378:metabolism; D005993:Glycerolphosphate Dehydrogenase / Q000302:isolation & purification / Q000378:metabolism*; D005994:Glycerophosphates / Q000096:biosynthesis*; D017014:Methanobacterium / Q000201:enzymology*; D009249:NADP / Q000378:metabolism; D010088:Oxidoreductases / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D010742:Phospholipid Ethers / Q000737:chemistry / Q000378:metabolism; D011817:Rabbits; D013237:Stereoisomerism; D014305:Triose-Phosphate Isomerase / Q000378:metabolism
chemical_list: D005994:Glycerophosphates; D010742:Phospholipid Ethers; D009249:NADP; D004099:Dihydroxyacetone Phosphate; D010088:Oxidoreductases; D005993:Glycerolphosphate Dehydrogenase; C098215:glycerol-1-phosphate dehydrogenase; D014305:Triose-Phosphate Isomerase