Isocratic high-performance liquid chromatographic method for quantitative determination of lysine, histidine and tyrosine in foods.
A method for the quantitative determination of lysine, histidine and tyrosine in foods based on pre-column derivatization with 5-dimethylaminonaphthalene-1-sulfonyl chloride (DnsCl) and reversed-phase liquid chromatography has been developed. Derivatization conditions, including DnsCl concentration, time, temperature, and buffer solution were studied. To establish the reliability of the proposed liquid chromatographic (LC) method, the precision and accuracy of the analyses were evaluated using samples of casein and lysozyme.
Sanz
M A
MA
Departamento de Nutrición y Bromatología, Universidad de Alcalá de Henares, Alcalá de Henares (Madrid), Spain.
Castillo
G
G
Hernández
A
A
D002364:Caseins / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000706:statistics & numerical data; D002852:Chromatography, Ion Exchange; D003619:Dansyl Compounds; D005504:Food Analysis / Q000379:methods*; D006639:Histidine / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D008239:Lysine / Q000032:analysis*; D009113:Muramidase / Q000032:analysis; D012680:Sensitivity and Specificity; D014443:Tyrosine / Q000032:analysis*
D002364:Caseins; D003619:Dansyl Compounds; D014443:Tyrosine; D006639:Histidine; D009113:Muramidase; D008239:Lysine; C029038:dansyl chloride