Cloning of rat GADD45 gene and induction analysis following ionizing radiation in vivo.
A gene encoded GADD45 was isolated from rat and revealed four exons along with a p53 binding consensus sequence and a putative AP-1 site in the third intron. This suggests that the rat GADD45 gene is also involved in the p53 signal pathway related to the G1 cell cycle checkpoint. The rat GADD45 mRNA was induced within 30 min in liver and increased as a function of gamma-irradiation. We found that mRNA expression differed substantially in a variety of tissues (brain, liver, kidney, and spleen). The finding of in vivo induction of GADD45 gene may provide insight into the role of GADD45 gene in DNA repair.
Yoshida
T
T
Division of Neurogerontology, University of Southern California, Los Angeles 90089, USA.
Okazaki
T
T
Hughes
P E
PE
Schneider
E L
EL
Mori
N
N
D000477:Alkylating Agents / Q000494:pharmacology; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D004249:DNA Damage; D005020:Ethyl Methanesulfonate / Q000494:pharmacology; D005720:Gamma Rays; D005786:Gene Expression Regulation / Q000187:drug effects / Q000528:radiation effects*; D005796:Genes / Q000235:genetics*; D047908:Intracellular Signaling Peptides and Proteins; D008099:Liver / Q000737:chemistry; D008297:Male; D008741:Methyl Methanesulfonate / Q000494:pharmacology; D008969:Molecular Sequence Data; D009928:Organ Specificity; D011506:Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D017422:Sequence Analysis, DNA; D000097642:GADD45 Proteins
D000477:Alkylating Agents; D005020:Ethyl Methanesulfonate; D047908:Intracellular Signaling Peptides and Proteins; D008741:Methyl Methanesulfonate; D011506:Proteins; D012333:RNA, Messenger; D000097642:GADD45 Proteins