Platelet-derived growth factor accelerates gastric epithelial restoration in a rabbit cultured cell model.
OBJECTIVE
Growth factors play an important role in gastric wo und repair. The aim of this study was to assess the role of platelet-derived growth factor (PDGF) in gastric epithelial restoration.


METHODS
PDGF-BB (1-50 ng/mL) was added to confluent cultures of rabbit gastric epithelial cells after wounding. Regrowth of the epithelial cells was monitored for 48 hours. The speed of cell migration was measured, and cell proliferation was detected by using a 5-bromodeoxyuridine (BrdU) staining technique. The labeling index was calculated for a 0.05-mm(2) area around the wound.


RESULTS
After wounding, cells at the wound edge formed lamellipodia and showed ruffling movements. The addition of PDGF-BB significantly accelerated cell migration and proliferation as well as gastric restoration. Migration speed was 21 microm/h in control cultures and 32 microm/h and 40 microm/h in cultures containing 10 ng/mL and 50 ng/mL of PDGF-BB, respectively. In control cultures, BrdU-positive cells were rarely detected in the initial 24 hours after wounding, and maximum labeling occurred at 36 hours (labeling index, 3.4%). Cultures containing PDGF-BB showed maximum labeling at 24 hours (labeling index, 6.9%).


CONCLUSIONS
PDGF-BB dose-dependently accelerated the migration rate and proliferation of cultured gastric epithelial cells after wounding. Therefore, PDGF-BB may play a role in gastric epithelial cell restoration during healing of gastric mucosal lesions.
Watanabe
S
S
Department of Gastroenterology, Juntendo University School of Medicine, Tokyo, Japan.
Wang
X E
XE
Hirose
M
M
Oide
H
H
Kitamura
T
T
Miwa
H
H
Miyazaki
A
A
Sato
N
N
D000818:Animals; D000077214:Becaplermin; D001973:Bromodeoxyuridine; D002455:Cell Division; D002465:Cell Movement; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004847:Epithelial Cells; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology / Q000502:physiology*; D020574:Proto-Oncogene Proteins c-sis; D011817:Rabbits; D013270:Stomach / Q000166:cytology*
D010982:Platelet-Derived Growth Factor; D020574:Proto-Oncogene Proteins c-sis; D000077214:Becaplermin; D001973:Bromodeoxyuridine