title: The novel nuclear gene DSS-1 of Saccharomyces cerevisiae is necessary for mitochondrial biogenesis.
abstract: A previously unknown nuclear gene DSS-1 from Saccharomyces cerevisiae was cloned and sequenced. The gene was isolated as a multicopy suppressor of a disruption of the SUV-3 gene coding for a DEAD/H box protein involved in processing and turnover of mitochondrial transcripts. The DSS-1 gene codes for a 970 amino-acid protein of molecular weight 111 kDa and is necessary for mitochondrial biogenesis. Amino-acid sequence analysis indicates the presence of motifs characteristic for Escherichia coli RNase II, the dis3 protein from Schizosaccharomyces pombe, the cyt4 protein participating in RNA processing and turnover in Neurospora crassa mitochondria, and the vacB protein from Shigella flexneri. We suggest that the DSS-1 protein may be a component of the mitochondrial 3'-5' exoribonuclease complex.
authors.affiliation: Department of Genetics, University of Warsaw, Poland.
authors.forename: A
authors.initials: A
authors.lastname: Dmochowska
authors.forename: P
authors.initials: P
authors.lastname: Golik
authors.forename: P P
authors.initials: PP
authors.lastname: Stepien
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002467:Cell Nucleus / Q000378:metabolism*; D003001:Cloning, Molecular; D053487:DEAD-box RNA Helicases; D005095:Exoribonucleases; D005656:Fungal Proteins / Q000235:genetics*; D005800:Genes, Fungal; D008928:Mitochondria / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D020365:RNA Helicases; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D017386:Sequence Homology, Amino Acid; D013489:Suppression, Genetic
chemical_list: D005656:Fungal Proteins; D029701:Saccharomyces cerevisiae Proteins; D005095:Exoribonucleases; C098317:DSS1 protein, S cerevisiae; C075918:SUV3 protein, S cerevisiae; D053487:DEAD-box RNA Helicases; D020365:RNA Helicases