Study of the de novo synthesis of cholesterol in the rat liver: a newly developed radiotracer technique, "TLC-autoradioluminography".
A possible evaluation technique was devised for examing timely cholesterol synthetic activity in the rat liver. The principle of the technique is to measure the incorporation rate of 14C atoms into a few target metabolites excreted in bile with time after an intraportal injection of [1,2-14C] acetate to the conscious rats. The measuring technique, TLC-autoradioluminography (TLC-ARLG), was devised for much more accurate, quantitative, simple and quick ultramicroanalysis of radioactive metabolites than ever before. Practically, a few microliters of the bile samples were analyzed by TLC before recording of a 2-dimensional radioactive image on the TLC plate. The incorporation ratio of 14C into cholesterol to succinic acid (C/S ratio) varied such that the value found in a 24-h fasting group was less than one-tenth of that in a sufficiently fed control group. For use as an antihyperglycemic drug, the bile C/S ratio in the nicotinic-acid-dosed group was approximately one-fifth of that in the control group.
Okuyama
M
M
Institute of Whole Body Metabolism, Chiba, Japan.
Tsunogai
M
M
Watanabe
N
N
Asakura
Y
Y
Shigematsu
A
A
D000085:Acetates / Q000378:metabolism; D000818:Animals; D000924:Anticholesteremic Agents / Q000494:pharmacology; D001345:Autoradiography / Q000379:methods*; D001646:Bile / Q000378:metabolism; D002784:Cholesterol / Q000096:biosynthesis* / Q000097:blood; D002855:Chromatography, Thin Layer; D066298:In Vitro Techniques; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D051381:Rats; D017208:Rats, Wistar
D000085:Acetates; D000924:Anticholesteremic Agents; D002784:Cholesterol