title: Quantitative analysis of synthetic human calcitonin by liquid chromatography-mass spectrometry.
abstract: The quantitative analysis of synthetic human calcitonin (hCT) by micro-liquid chromatography-electrospray ionization mass spectrometry (micro-LC-ESI-MS) is reported. hCT was extracted from plasma by an immobilized antibody column and separated by a micro-LC system. The molecular mass of hCT is 3417, and m/z 1140, corresponding to the [M + 3H]3+ ion, was observed by ESI-MS. This ion was monitored in the selected-ion monitoring mode; rat calcitonin, which is highly homologous to hCT, was used as an internal standard. The calibration curve for the quantification of hCT was linear in the range 10 ng/ml to 1 microgram/ml of plasma.
authors.affiliation: Analytical Laboratory, Sankyo Co., Ltd., Tokyo, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Kobayashi
authors.forename: M
authors.initials: M
authors.lastname: Kanai
authors.forename: K
authors.initials: K
authors.lastname: Seta
authors.forename: K
authors.initials: K
authors.lastname: Nakamura
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002116:Calcitonin / Q000097:blood*; D002138:Calibration; D002853:Chromatography, Liquid; D006801:Humans; D007202:Indicators and Reagents; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D051381:Rats; D011994:Recombinant Proteins / Q000097:blood; D012015:Reference Standards
chemical_list: D007202:Indicators and Reagents; D011994:Recombinant Proteins; D002116:Calcitonin