Ionic transport in the plasma membrane of carrot protoplasts from embryogenic cell-suspension cultures.
Ionic transport properties of protoplasts obtained from embryogenic carrot suspension cells were studied by the patch-clamp technique. In the whole-cell configuration, carrot protoplasts presented macroscopic time-dependent outward currents, showing kinetics of activation which did not depend appreciably on the amplitude of the stimulus. Time- and voltage-dependent whole-cell inward rectifying currents as well as instantaneous non-selective currents were also observed. Both time-dependent inward and outward currents are carried by potassium ions. In a cell-attached configuration, two types of single-channel signals, displaying conductances of 10 and 17 pS, were observed; the instantaneous 10 pS channel was also present in outside-out excised patches.
Bregante
M
M
Istituto di Cibernetica e Biofisica, CNR, Genova, Italy.
Gambale
F
F
LoSchiavo
F
F
D002462:Cell Membrane / Q000378:metabolism*; D018552:Daucus carota / Q000196:embryology / Q000378:metabolism*; D004553:Electric Conductivity; D017136:Ion Transport; D008564:Membrane Potentials; D018408:Patch-Clamp Techniques; D011188:Potassium / Q000378:metabolism*; D015221:Potassium Channels / Q000378:metabolism*; D011523:Protoplasts / Q000378:metabolism*
D015221:Potassium Channels; D011188:Potassium