Clustering of Cx43 cell-to-cell channels into gap junction plaques: regulation by cAMP and microfilaments.
Cell-to-cell channels are often seen clustered at cell-cell contacts into the so-called gap junction plaques. The mechanism of this clustering is unknown. We show that the clustering of cell-to-cell channels composed of connexin43 is induced by elevation of cyclic AMP. The cAMP-induced clustering is enhanced by inhibition of glycosylation and abolished by disruption of microfilaments. Channel clustering thus seems to be regulated by cAMP and glycosylation and to involve microfilaments.
Wang
Y
Y
Department of Physiology and Biophysics, University of Miami School of Medicine, Florida 33101, USA.
Rose
B
B
D008841:Actin Cytoskeleton / Q000502:physiology*; D000818:Animals; D006528:Carcinoma, Hepatocellular; D002450:Cell Communication / Q000502:physiology*; D005576:Colforsin / Q000494:pharmacology; D018031:Connexin 43 / Q000096:biosynthesis / Q000378:metabolism*; D000242:Cyclic AMP / Q000502:physiology*; D003572:Cytochalasins / Q000494:pharmacology; D004721:Endoplasmic Reticulum / Q000378:metabolism; D017629:Gap Junctions / Q000378:metabolism*; D006031:Glycosylation; D006056:Golgi Apparatus / Q000378:metabolism; D011506:Proteins / Q000378:metabolism; D051381:Rats; D014407:Tumor Cells, Cultured / Q000378:metabolism / Q000648:ultrastructure
D018031:Connexin 43; D003572:Cytochalasins; D011506:Proteins; D005576:Colforsin; D000242:Cyclic AMP