title: Thermotoga subterranea sp. nov., a new thermophilic bacterium isolated from a continental oil reservoir.
abstract: A thermophilic, strictly anaerobic bacterium, designated strain SL1, was isolated from a deep, continental oil reservoir in the East Paris Basin (France). This organism grew between 50 and 75 degrees C, with an optimum at 70 degrees C. It was inhibited by elemental sulfur and was able to reduce cystine and thiosulfate to hydrogen sulfide. The G+C content (40 mol%), the presence of a lipid structure unique to the genus Thermotoga, and the 16S rRNA sequence of strain SL1 indicated that the isolate belongs to the genus Thermotoga. Based on DNA-DNA hybridization, isolate SL1 does not show species-level similarity with the recognized species T. maritima, T. neapolitana, and T. thermarum. Based on this description of strain SL1, we propose the recognition of a new species: Thermotoga subterranea.
authors.affiliation: Station Biologique, UPR 9042, Centre National de la Recherche Scientifique Roscoff, France.
authors.forename: C
authors.initials: C
authors.lastname: Jeanthon
authors.forename: A L
authors.initials: AL
authors.lastname: Reysenbach
authors.forename: S
authors.initials: S
authors.lastname: L'Haridon
authors.forename: A
authors.initials: A
authors.lastname: Gambacorta
authors.forename: N R
authors.initials: NR
authors.lastname: Pace
authors.forename: P
authors.initials: P
authors.lastname: Glénat
authors.forename: D
authors.initials: D
authors.lastname: Prieur
mesh_terms: D001482:Base Composition; D001483:Base Sequence; D003553:Cystine / Q000378:metabolism; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D006089:Gram-Negative Anaerobic Bacteria / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism; D006358:Hot Temperature; D008055:Lipids / Q000032:analysis; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D010578:Petroleum / Q000382:microbiology*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013455:Sulfur / Q000378:metabolism; D013885:Thiosulfates / Q000378:metabolism
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D008055:Lipids; D010578:Petroleum; D012336:RNA, Ribosomal, 16S; D013885:Thiosulfates; D003553:Cystine; D013455:Sulfur