title: Pituitary activin receptor subtypes and follistatin gene expression in female rats: differential regulation by activin and follistatin.
abstract: The activins, hormones produced in the gonads and extragonadal tissues (including the pituitary), rapidly increase FSH beta messenger RNA (mRNA) and FSH secretion. In the rat, activin acts via a family of activin receptor (ActR) subunits that includes at least one type I (ActRI or ALK-2) and two homologous type II (IIA and IIB) subunits. We have previously reported that ActRIIA mRNA rises after ovariectomy (OVX). Potentially, the OVX-induced increases in ActR mRNAs could result from altered activin or the activin-binding protein follistatin. It was the purpose of the current studies to determine whether activin and/or follistatin regulated activin receptor subunit mRNAs. Adult female rat pituitaries were dissociated and plated for 48 h, transferred to wells containing follistatin or activin for 2 or 24 h, then RNA extracted for measurement of ActRI, IIA, and IIB and follistatin mRNAs. All three ActR mRNAs were easily detectable in pituitary RNA, with the relative abundance of ActRI > IIA >> IIB (18:9:1). Between 2-24 h, levels of all three ActR mRNAs increased 2- to 3-fold in wells containing medium alone, whereas levels of follistatin mRNA were unchanged. Follistatin significantly reduced FSH secretion and follistatin mRNA, but not the ActR mRNAs. Activin increased ActRI (4-fold, at 2 h), ActRIIB (2-fold, at 24 h), and follistatin (2-fold, at 24 h) mRNAs and FSH release (2-fold, at 24 h), but did not alter ActRIIA mRNA levels. We conclude that 1) pituitary ActR mRNA expression is under inhibitory tone in vivo, as suggested by the effect of pituitary removal and cell dispersion and an earlier report after OVX. 2) Pituitary-derived activin stimulates follistatin (but not ActR) mRNA production, and additional increases in follistatin mRNA can be induced by exogenous activin. 3) Higher concentrations of activin differentially regulate pituitary ActR mRNA expression, suggesting that activin exerts a positive feedback effect on its own receptor.
authors.affiliation: University of Virginia Health Science Center, Charlottesville 22908, USA.
authors.forename: A C
authors.initials: AC
authors.lastname: Dalkin
authors.forename: D J
authors.initials: DJ
authors.lastname: Haisenleder
authors.forename: M
authors.initials: M
authors.lastname: Yasin
authors.forename: J T
authors.initials: JT
authors.lastname: Gilrain
authors.forename: J C
authors.initials: JC
authors.lastname: Marshall
mesh_terms: D029404:Activin Receptors; D028341:Activins; D000818:Animals; D001483:Base Sequence; D005260:Female; D038681:Follistatin; D005786:Gene Expression Regulation; D006023:Glycoproteins / Q000235:genetics* / Q000494:pharmacology*; D007265:Inhibins / Q000037:antagonists & inhibitors / Q000378:metabolism / Q000494:pharmacology*; D008969:Molecular Sequence Data; D010902:Pituitary Gland / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017978:Receptors, Growth Factor / Q000235:genetics*
chemical_list: D038681:Follistatin; D006023:Glycoproteins; D017978:Receptors, Growth Factor; D028341:Activins; D007265:Inhibins; D029404:Activin Receptors