The biosynthesis of Lewis X in Helicobacter pylori.
The biosynthesis of the Lewis X determinant (Gal beta 1-4 [Fuc alpha 1-3]GlcNAc beta-) in three strains of Helicobacter pylori has been investigated. Strains UA 861, UA 802 and UA 1182 contain alpha 1,3 fucosyltransferase and beta 1,4 galactosyltransferase activities that synthesize the Lewis X structure by the transfer of monosaccharides from GDP-fucose and UDP-galactose donors, respectively. The enzyme reaction products that formed were characterized by capillary zone electrophoresis and by 1H-NMR spectroscopy. The biosynthetic pathway is therefore identical to that found in humans. In the three strains, the fucosyltransferase and galactosyltransferase activities differed in various cellular fractions. Km values for their donor and acceptor substrates also differed.
Chan
N W
NW
Department of Chemistry, University of Alberta, Edmonton, Canada.
Stangier
K
K
Sherburne
R
R
Taylor
D E
DE
Zhang
Y
Y
Dovichi
N J
NJ
Palcic
M M
MM
D002240:Carbohydrate Sequence; D005647:Fucosyltransferases / Q000378:metabolism; D006154:Guanosine Diphosphate Fucose / Q000378:metabolism; D016480:Helicobacter pylori / Q000302:isolation & purification / Q000378:metabolism* / Q000648:ultrastructure; D006801:Humans; D007700:Kinetics; D016256:Lewis X Antigen / Q000096:biosynthesis* / Q000737:chemistry; D016253:Microscopy, Immunoelectron; D008969:Molecular Sequence Data; D009237:N-Acetyllactosamine Synthase / Q000378:metabolism; D013379:Substrate Specificity; D014531:Uridine Diphosphate Galactose / Q000378:metabolism
D016256:Lewis X Antigen; D006154:Guanosine Diphosphate Fucose; D014531:Uridine Diphosphate Galactose; D005647:Fucosyltransferases; C031692:galactoside 3-fucosyltransferase; D009237:N-Acetyllactosamine Synthase