Structure-based sequence alignment of elongation factors Tu and G with related GTPases involved in translation.
The G domain and domain II in the crystal structure of Thermus thermophilus elongation factor G (EF-G) were compared with the homologous domains in Thermus aquaticus elongation factor Tu (EF-Tu). Sequence alignment derived from the structural superposition was used to define conserved sequence elements in domain II. These elements and previously known conserved sequence elements in the G domain were used to guide the alignment of the sequences of Sulfolobus acidocaldarius elongation factor 2, human elongation factor 2, and Escherichia coli initiation factor 2 and release factor 3 to the aligned sequences of EF-G and EF-Tu. This alignment, which deviates from previously published alignments, has evolutionary implications and leads to alternative interpretations of biochemical data concerning the interaction of elongation factors with the alpha-sarcin/ricin region of the ribosome. A single conserved sequence motif in domain II was identified and used to further characterize the GTPase subfamily of translation factors and related proteins. It was shown that the motif is found in most if not all the members of the family. Apparently, the common characteristic of these GTPases is an extensive consensus structural unit that possibly accounts for a similar interaction with the ribosome and is composed of two domains homologous to the G domain and domain II in EF-Tu and EF-G.
Avarsson
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A
Department of Molecular Biophysics, University of Lund, Sweden.
D000595:Amino Acid Sequence; D020963:GTP Phosphohydrolase-Linked Elongation Factors / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D020653:Peptide Elongation Factor G; D010444:Peptide Elongation Factor Tu / Q000235:genetics*; D010445:Peptide Elongation Factors / Q000235:genetics*; D014176:Protein Biosynthesis; D011487:Protein Conformation; D016415:Sequence Alignment; D016963:Thermus thermophilus / Q000235:genetics*
D020653:Peptide Elongation Factor G; D010445:Peptide Elongation Factors; D020963:GTP Phosphohydrolase-Linked Elongation Factors; D010444:Peptide Elongation Factor Tu