Characterization of neuraminidases produced by various serotypes of Pasteurella multocida.
Neuraminidases produced by 16 strains of Pasteurella multocida (serotypes 1 to 16) were characterized by molecular weight, substrate specificity, and antigenic identity. After growth in a chemically defined medium, stage I (lyophilized) culture supernatants were assayed for activity with N-acetylneuramin lactose, human alpha-1-acid glycoprotein, fetuin, colominic acid, and bovine submaxillary mucin. Neuraminidase produced by P. multocida A:3 was purified by a combination of salt fractionation, ion-exchange chromatography on DEAE-Sephacel, and gel filtration on Sephadex G-200. Purified P. multocida A:3 neuraminidase was employed to immunize rabbits, and the resulting antiserum reduced the activity of the P. multocida A:3 enzyme by 40.3%. This antiserum also reduced the activities of the neuraminidases produced by other serotypes by between 30.8 and 59.6%. Molecular weight estimates of the neuraminidases produced by the various serotypes were obtained by gel filtration chromatography on Sephadex G-200. Each of the 16 serotypes examined produced a neuraminidase with a molecular weight of approximately 500,000. In addition, all 16 high-molecular-weight neuraminidases showed similar substrate specificities. On the basis of these data, it appears that the high-molecular-weight neuraminidases produced by different P. multocida serotypes are quite similar.
Straus
D C
DC
Department of Microbiology and Immunology, Texas Tech University Health Sciences Center, Lubbock, Texas 79430, USA.
Jolley
W L
WL
Purdy
C W
CW
D000818:Animals; D002417:Cattle; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D008970:Molecular Weight; D009439:Neuraminidase / Q000276:immunology* / Q000302:isolation & purification / Q000378:metabolism; D016979:Pasteurella multocida / Q000145:classification / Q000201:enzymology* / Q000254:growth & development; D011817:Rabbits; D012703:Serotyping; D013379:Substrate Specificity
D007106:Immune Sera; D009439:Neuraminidase