title: Characterization of 2 aquareovirus proteins.
abstract: The protein products of aquareovirus genome segments 10 and 11 were investigated. The genome segment 10 encodes the major outer capsid protein (VP7) of aquareovirus. The VP7 protein has a molecular weight of 35 kDa and was found to be glycosylated. The electrophoretic mobility of VP7 in SDS-PAGE was unaltered when untreated and deglycosylated proteins were compared, indicating the presence of short oligosaccharide chains. Selective binding of lectins with well-defined sugar specificities suggest that most of the short N-linked carbohydrate chains of VP7 contain galactose residues. Genome segment 11 encoded 2 non-structural proteins (NS29 and NS15). Partial protease mapping indicated that the NS29 and NS15 proteins are derived from initiation of protein synthesis at 2 distinct sites.
authors.affiliation: College of Veterinary Medicine, Maryland Agricultural Experiment Station, University of Maryland, College Park 20742, USA.
authors.forename: S K
authors.initials: SK
authors.lastname: Samal
authors.forename: K
authors.initials: K
authors.lastname: Subramanian
authors.forename: B
authors.initials: B
authors.lastname: Lupiani
mesh_terms: D000818:Animals; D000956:Antigens, Viral; D001492:Bass / Q000821:virology*; D002213:Capsid / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism; D036022:Capsid Proteins; D002460:Cell Line; D004625:Embryo, Nonmammalian; D016679:Genome, Viral; D006031:Glycosylation; D014176:Protein Biosynthesis; D011499:Protein Processing, Post-Translational; D012330:RNA, Double-Stranded / Q000302:isolation & purification / Q000378:metabolism; D012367:RNA, Viral / Q000302:isolation & purification / Q000378:metabolism; D012087:Reoviridae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D012474:Salmon
chemical_list: D000956:Antigens, Viral; D036022:Capsid Proteins; D012330:RNA, Double-Stranded; D012367:RNA, Viral; C050779:VP7 protein, Rotavirus