[Induction of antibiotic formation by inactive cultures of actinomycetes. A mutant strain of Streptomyces helvaticus, a new producer of bruneomycin].
After exposure of an inactive actinomycete to ethidium bromide a stable mutant producing an antibiotic on a solid medium was isolated. The exposure to methylnitrosoguanidine provided the isolation of a more productive variant synthesizing the antibiotic in a liquid medium. By UV, IR, NMR and mass spectroscopy the antibiotic was identified as bruneomycin (streptonigrin). The taxonomic investigation of the culture showed that it belongs to Streptomyces helvaticus which is a new culture producing bruneomycin (streptonigrin). Previously it did not synthesize the antibiotic.
Malkina
N D
ND
Dudnik
Iu V
IuV
Lysenkova
L N
LN
Terekhova
L P
LP
Katrukha
G S
GS
D000903:Antibiotics, Antineoplastic / Q000096:biosynthesis*; D004996:Ethidium / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology; D009154:Mutation; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet; D013302:Streptomyces / Q000145:classification / Q000187:drug effects* / Q000502:physiology; D013308:Streptonigrin / Q000096:biosynthesis*
D000903:Antibiotics, Antineoplastic; D008769:Methylnitronitrosoguanidine; D013308:Streptonigrin; D004996:Ethidium