Identification of macrophage migration inhibitory factor (MIF) in human skin and its immmunohistochemical localization.
The presence and tissue localization of macrophage migration inhibitory factor (MIF) in human skin were examined. Reverse transcription-polymerase chain reaction analysis revealed that MIF mRNA was expressed in both surgically obtained normal human epidermis and primary cultured human keratinocytes. The expression of MIF was further confirmed by Western blot analysis, which demonstrated a single band at about 12.5 kDa using a polyclonal antibody against human recombinant MIF. Immunohistochemical studies showed that MIF existed in human epidermis, especially in the basal layer. The pathophysiological role of MIF in human skin remains undefined; however, the present results indicate that MIF may play an important role in immunity, inflammation and cellular differentiation of epidermal cells.
Shimizu
T
T
Department of Dermatology, School of Medicine, Hokkaido University, Sapporo, Japan.
Ohkawara
A
A
Nishihira
J
J
Sakamoto
W
W
D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D002454:Cell Differentiation; D002478:Cells, Cultured; D017931:DNA Primers; D000078404:Epidermal Cells; D004817:Epidermis / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D007109:Immunity; D007150:Immunohistochemistry; D007249:Inflammation; D015603:Keratinocytes / Q000166:cytology* / Q000378:metabolism*; D008263:Macrophage Migration-Inhibitory Factors / Q000032:analysis* / Q000096:biosynthesis*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D011817:Rabbits / Q000276:immunology; D012016:Reference Values; D012867:Skin / Q000166:cytology* / Q000378:metabolism
D017931:DNA Primers; D008263:Macrophage Migration-Inhibitory Factors; D012333:RNA, Messenger