Molecular cloning and expression of receptor peptides that block human cytomegalovirus/cell fusion.
Clones expressing a partial human cytomegalovirus putative fusion receptor were selected by binding specifically to monoclonal anti-idiotypic antibodies that mimic glycoprotein H. cDNA was isolated from 2 of the clones (131 and 611) and fused in frame with the glutathione S-transferase gene in a pGEX-4T-1 vector. Two purified peptides (FR131 and FR611) were produced: both were shown to bind specifically to the monoclonal anti-idiotypic antibodies and inhibit virus/cell fusion and viral plaque formation in a specific and dose-dependent manner. This is the first demonstration of cloned peptides encoding a putative cell membrane receptor that are able to block cytomegalovirus infection.
Baldwin
B R
BR
Molecular and Cell Biology Program, Division of Infectious Diseases, University of Maryland School of Medicine, Baltimore 21201, USA.
Kleinberg
M
M
Keay
S
S
D000888:Antibodies, Anti-Idiotypic / Q000494:pharmacology; D000911:Antibodies, Monoclonal; D015153:Blotting, Western; D002459:Cell Fusion; D002478:Cells, Cultured; D003001:Cloning, Molecular; D003587:Cytomegalovirus / Q000502:physiology*; D015870:Gene Expression; D005982:Glutathione Transferase / Q000096:biosynthesis; D006801:Humans; D008168:Lung; D008565:Membrane Proteins / Q000187:drug effects / Q000502:physiology; D010446:Peptide Fragments / Q000096:biosynthesis / Q000494:pharmacology*; D011991:Receptors, Virus / Q000187:drug effects / Q000502:physiology*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000187:drug effects / Q000378:metabolism; D010948:Viral Plaque Assay
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D008565:Membrane Proteins; D010446:Peptide Fragments; D011991:Receptors, Virus; D011993:Recombinant Fusion Proteins; C064084:cytomegalovirus receptor; D005982:Glutathione Transferase