Transgenic insertional mutagenesis. Applications to inner-ear genetics.
With the advent of new and sophisticated molecular biological techniques, tremendous recent strides have been made in inner-ear genetics. The complexity of the mammalian genome and the paucity of genetic markers, however, have impeded rapid progress in the characterization of many inner-ear mutations at the molecular level. Transgenic technology, through insertional mutagenesis and targeted gene inactivation by homologous recombination, has provided a direct approach to the characterization of mutant phenotypes at the level of the gene.
Friedman
R A
RA
Division of Otolaryngology/Head and Neck Surgery, University of California, San Diego, USA.
D000818:Animals; D003638:Deafness / Q000235:genetics*; D007758:Ear, Inner / Q000002:abnormalities; D018390:Gene Targeting / Q000379:methods*; D018014:Gene Transfer Techniques; D018095:Germ-Line Mutation; D006801:Humans; D051379:Mice; D008817:Mice, Mutant Strains; D008822:Mice, Transgenic; D016254:Mutagenesis, Insertional / Q000379:methods*; D011995:Recombination, Genetic