title: Endogenous opiates do not modulate LPS-induced alterations in carbohydrate metabolism.
abstract: This study aimed to determine the contribution of endogenous opiates to the hormonal and glucose metabolic response to lipopolysaccharide (LPS). Rats were infused with naloxone (NAL) (32 micrograms/h) for 2 h prior to the injection of LPS (100 micrograms/100 g body weight) and hemodynamic, hormonal, and metabolic parameters were determined. NAL produced no detectable alterations in any of the parameters assessed. LPS transiently decreases (26%) mean arterial blood pressure (MABP) and increased plasma glucose concentration (100-130%), glucose rate of appearance (50-100%), and glucose rate of disappearance (50-100%). NAL did not alter the LPS-induced drop in mean arterial blood pressure or the glucose response to LPS. LPS reduced plasma insulin (54%), and increased glucagon (270%), corticosterone (180%), and tumor necrosis factor concentrations in plasma (peak 3200-4600 pg/mL at 90 min), with no modification by NAL pretreatment. These results suggest a lack of involvement of endogenous opiate pathways in the glucose metabolic and hormonal responses to LPS.
authors.affiliation: Department of Surgery, State University of New York at Stony Brook, 11794-8191, USA.
authors.forename: S
authors.initials: S
authors.lastname: Bundz
authors.forename: P E
authors.initials: PE
authors.lastname: Molina
authors.forename: C H
authors.initials: CH
authors.lastname: Lang
authors.forename: N N
authors.initials: NN
authors.lastname: Abumrad
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000187:drug effects / Q000378:metabolism*; D006003:Glycogen / Q000378:metabolism; D006439:Hemodynamics / Q000187:drug effects; D006728:Hormones / Q000378:metabolism; D007773:Lactates / Q000378:metabolism; D019344:Lactic Acid; D008070:Lipopolysaccharides / Q000633:toxicity*; D008297:Male; D009270:Naloxone / Q000494:pharmacology; D009292:Narcotic Antagonists / Q000494:pharmacology; D018847:Opioid Peptides / Q000502:physiology*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013312:Stress, Physiological / Q000378:metabolism* / Q000503:physiopathology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D001786:Blood Glucose; D006728:Hormones; D007773:Lactates; D008070:Lipopolysaccharides; D009292:Narcotic Antagonists; D018847:Opioid Peptides; D014409:Tumor Necrosis Factor-alpha; D019344:Lactic Acid; D009270:Naloxone; D006003:Glycogen