Cytotoxic, nuclear, and growth inhibitory effects of photodynamic drugs on pancreatic carcinoma cells.
The light-activated drugs AlPcS4 and T4MPyP were studied in a pancreatic carcinoma cell line for their effects on DNA integrity, cell division, proliferation, and survival. The micronucleus assay measured nuclear changes and also the number of actively dividing cells while, under similar conditions, the MTT assay measured cell survival. When tumour cells were exposed to light, pre-treatment with AlPcS4 induced more micronuclei than did T4MPyP at the same levels of cell division and survival. Both drugs showed a correlation between phototoxicity and changes to DNA integrity so establishing micronuclei formation as an important indicator of photodynamic drug action on tumour cells.
Ward
A J
AJ
Department of Pharmacology, University of Cambridge, UK.
Matthews
E K
EK
D000818:Animals; D018270:Carcinoma, Ductal, Breast / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D006224:Cricetinae; D004273:DNA, Neoplasm / Q000187:drug effects / Q000378:metabolism; D007211:Indoles / Q000494:pharmacology*; D008647:Mesocricetus; D048629:Micronuclei, Chromosome-Defective / Q000187:drug effects; D015162:Micronucleus Tests; D009942:Organometallic Compounds / Q000494:pharmacology*; D010190:Pancreatic Neoplasms / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism; D010778:Photochemotherapy; D011166:Porphyrins / Q000494:pharmacology*; D011838:Radiation-Sensitizing Agents / Q000494:pharmacology*; D013778:Tetrazolium Salts; D013844:Thiazoles; D014407:Tumor Cells, Cultured
D004273:DNA, Neoplasm; D007211:Indoles; D009942:Organometallic Compounds; D011166:Porphyrins; D011838:Radiation-Sensitizing Agents; D013778:Tetrazolium Salts; D013844:Thiazoles; C048577:aluminum tetrasulfophthalocyanine; C021096:tetra(4-N-methylpyridyl)porphine; C022616:thiazolyl blue