A high-performance liquid chromatographic assay for the determination of itraconazole concentration using solid-phase extraction and small sample volume.
Itraconazole has been shown to be highly effective against a broad spectrum of fungi, particularly aspergillus. In order to achieve the desired pharmacological effect, the itraconazole plasma concentration should be > 250 ng/ml. We developed a high-performance liquid chromatography assay for the rapid determination of plasma itraconazole concentration using solid-phase extraction and small sample volume. The assay possessed linearity up to 5,000 ng/ml, sensitivity of 50 ng/ml, average recovery of 100.8%, and run-to-run precision (n = 10) for concentrations of 100, 200, and 500 ng/ml of 12, 6.6, and 5.8%, respectively. Furthermore, the assay proved to be free of interference from 48 commonly prescribed and some over-the-counter medications. We conclude that the method described here is ideally suited for the therapeutic monitoring of itraconazole.
Rifai
N
N
Department of Laboratory Medicine, Children's Hospital, Boston, Massachusetts 02115, USA.
Sakamoto
M
M
Platt
O
O
Brugnara
C
C
D000097:Acetonitriles / Q000737:chemistry; D000935:Antifungal Agents / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004347:Drug Interactions; D016903:Drug Monitoring; D006801:Humans; D017964:Itraconazole / Q000097:blood*; D012044:Regression Analysis
D000097:Acetonitriles; D000935:Antifungal Agents; D017964:Itraconazole; C032159:acetonitrile