A comparative analysis of the photosensitized inhibition of growth-factor regulated protein kinases by hypericin-derivatives.
The photodynamic inhibitory effect of hypericin and a number of hypericin-derivatives were investigated in vitro using numerous growth-factor regulated protein kinases including receptor-bound (Insulin-R, EGF-R) and non-receptor (Lyn, c-Fgr, CSK, Syk) protein tyrosine kinases as well as Ser/Thr (PK-C, protein kinase CK-2, CK-1) protein kinases. Modification of the hypericin structure altered significantly the specificity of the protein kinase inhibition. In particular, methylation or attachment of long lipophilic chains to both methyl groups of the hypericin molecule strongly enhanced the specificity toward PK-C.
Agostinis
P
P
Afdeling Biochemie, Faculteit der Geneeskunde, Katholieke Universiteit, Leuven, Belgium.
Donella-Deana
A
A
Cuveele
J
J
Vandenbogaerde
A
A
Sarno
S
S
Merlevede
W
W
de Witte
P
P
D000818:Animals; D000873:Anthracenes; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D066246:ErbB Receptors / Q000037:antagonists & inhibitors; D006133:Growth Substances / Q000494:pharmacology*; D015394:Molecular Structure; D010569:Perylene / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000737:chemistry / Q000494:pharmacology*; D047428:Protein Kinase Inhibitors; D011494:Protein Kinases / Q000378:metabolism; D011809:Quinones / Q000494:pharmacology; D011972:Receptor, Insulin / Q000037:antagonists & inhibitors; D011994:Recombinant Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism; D013329:Structure-Activity Relationship
D000873:Anthracenes; D004791:Enzyme Inhibitors; D006133:Growth Substances; D017319:Photosensitizing Agents; D047428:Protein Kinase Inhibitors; D011809:Quinones; D011994:Recombinant Proteins; D010569:Perylene; C004965:hypericin; D011494:Protein Kinases; D066246:ErbB Receptors; D011972:Receptor, Insulin