Determination of ionophores in the tissues of food animals by liquid chromatography.
A liquid chromatographic method for determining residues of ionophores in bovine, porcine, and avian tissues is described. Tissues were extracted with iso-octane-ethyl acetate and the extracts purified on silica solid-phase extraction columns. Monensin, narasin and salinomycin were detected by UV absorbance following post-column derivatization with vanillin, and lasalocid was detected underivatized using fluorescence. In muscle, kidney and fat, all drugs were determined from a single sample extract. In liver, a separate extraction was done to determine lasalocid. The detection limit for lasalocid, narasin and salinomycin was 5 ppb; for monensin it was 2 ppb. Average recoveries were: lasalocid-71.0%; monensin-94.3%; salinomycin-97.2%; narasin-94.1%.
Gerhardt
G
G
Health of Animals Laboratory, Agriculture and Agri-Food Canada, Saskatoon, Saskatchewan, Canada.
Salisbury
C D
CD
Campbell
H M
HM
D000273:Adipose Tissue / Q000737:chemistry; D000818:Animals; D001547:Benzaldehydes / Q000032:analysis; D002417:Cattle; D002645:Chickens; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003049:Coccidiostats / Q000032:analysis; D005504:Food Analysis / Q000379:methods*; D007476:Ionophores / Q000032:analysis*; D007668:Kidney / Q000737:chemistry; D007832:Lasalocid / Q000032:analysis; D008099:Liver / Q000737:chemistry; D008985:Monensin / Q000032:analysis; D009132:Muscles / Q000737:chemistry; D011714:Pyrans / Q000032:analysis; D013552:Swine; D000095662:Polyether Polyketides
D001547:Benzaldehydes; D003049:Coccidiostats; D007476:Ionophores; D007832:Lasalocid; D008985:Monensin; D011714:Pyrans; C010327:salinomycin; D000095662:Polyether Polyketides; C100058:vanillin; C013612:narasin