Enhanced effectiveness of dimethyl-4,4'-dimethoxy-5,6,5',6'-dimethylene dioxybiphenyl-2,2'-dicarboxylate in combination with garlic oil against experimental hepatic injury in rats and mice.
The present study was designed to evaluate the effects of dimethyl-4,4'-dimethoxy-5,6,5',6'-dimethylene dioxybiphenyl-2,2'-dicarboxylate (PMC) in combination with garlic oil against chemical-induced hepatic injury in rats and mice. Rats insulted with carbon tetrachloride were concomitantly treated with PMC and/or garlic oil (50 and 100 mg kg-1, respectively) for four weeks. Whereas treatment of animals with garlic oil alone was ineffective in suppressing carbon tetrachloride-induced hepatotoxicity, administration of PMC in combination with garlic oil more effectively protected the liver against the carbon tetrachloride-induced insult than PMC alone, as monitored by serum aminotransferase activity. Hepatoprotective effects of the formulation were further supported by the changes in the numbers of Kupffer cells and dead hepatocytes. Although prior treatment of rats with PMC for three days failed to protect hepatotoxicity elicited by allyl alcohol, the formulation of PMC and garlic oil was capable of blocking allyl alcohol-induced hepatotoxicity by approximately 40%. To further examine the effect of the agents on lipid metabolism in the liver, hepatic triglycerides and cholesterol contents were assessed in mice after a diet containing PMC and/or garlic oil for one week followed by a single dose of carbon tetrachloride. Garlic oil appeared to be more effective in bringing hepatic lipid levels to those of control than PMC alone. Treatment of animals with PMC in combination with garlic oil synergistically improved chemical-induced impairment of hepatic triglycerides and cholesterol.(ABSTRACT TRUNCATED AT 250 WORDS)
Kim
S G
SG
College of Pharmacy, Duksung Women's University, Seoul, Korea.
Nam
S Y
SY
Chung
H C
HC
Hong
S Y
SY
Jung
K H
KH
D000433:1-Propanol / Q000008:administration & dosage / Q000633:toxicity; D000284:Administration, Oral; D000498:Allyl Compounds; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000494:pharmacology*; D002251:Carbon Tetrachloride / Q000633:toxicity; D016923:Cell Death / Q000187:drug effects; D056486:Chemical and Drug Induced Liver Injury; D002784:Cholesterol / Q000378:metabolism; D004149:Dioxoles / Q000008:administration & dosage / Q000494:pharmacology*; D004357:Drug Synergism; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000494:pharmacology*; D007728:Kupffer Cells / Q000187:drug effects / Q000473:pathology; D008099:Liver / Q000187:drug effects* / Q000293:injuries / Q000378:metabolism; D008107:Liver Diseases / Q000517:prevention & control*; D008297:Male; D051379:Mice; D020005:Propanols; D051381:Rats; D017207:Rats, Sprague-Dawley; D013440:Sulfides / Q000008:administration & dosage / Q000494:pharmacology*; D000637:Transaminases / Q000097:blood; D014280:Triglycerides / Q000378:metabolism
D000498:Allyl Compounds; D000975:Antioxidants; D004149:Dioxoles; D004365:Drugs, Chinese Herbal; D020005:Propanols; D013440:Sulfides; D014280:Triglycerides; C006463:allyl alcohol; C038491:allyl sulfide; C041398:7,7'-dimethoxy-(4,4'-bi-1,3-benzodioxole)-5,5'-dicarboxylic acid dimethyl ester; D000433:1-Propanol; D002784:Cholesterol; D002251:Carbon Tetrachloride; D000637:Transaminases