title: Tumor necrosis factor production in cats in response to lipopolysaccharide: an in vivo and in vitro study.
abstract: Supernatants from feline peritoneal exudate cells (PECs) exposed to lipopolysaccharde (LPS) produced significantly (P < 0.05) more tumor necrosis factor (TNF) activity than supernatants from cells exposed to media. An in vitro LPS response was obtained following incubation of whole blood with 10 micrograms ml-1 LPS for 2 h. Intravenous infusion of LPS (750 micrograms kg-1 rapidly increased plasma TNF activity to a maximum at 60 min after initiation of LPS infusion. By 180 min, TNF activity returned to baseline. Cats produce TNF in response to LPS in a manner similar to other species. Measurement of TNF activity in plasma or in LPS-stimulated whole blood are methods to further characterize the inflammatory response in feline diseases.
authors.affiliation: University of Pennsylvania, Department of Clinical Studies-Philadelphia 19104-6010, USA.
authors.forename: C M
authors.initials: CM
authors.lastname: Otto
authors.forename: C A
authors.initials: CA
authors.lastname: Rawlings
mesh_terms: D000818:Animals; D001769:Blood / Q000276:immunology; D002371:Cat Diseases / Q000276:immunology; D002415:Cats / Q000276:immunology*; D066298:In Vitro Techniques; D007262:Infusions, Intravenous; D008070:Lipopolysaccharides / Q000008:administration & dosage / Q000494:pharmacology*; D010529:Peritoneal Cavity / Q000166:cytology; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*
chemical_list: D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha