title: DNA-nuclear matrix interactions analyzed by cross-linking reactions in intact nuclei from avian liver.
abstract: To detect the interactions of DNA with the nuclear matrix proteins, DNA-protein cross-linkages were induced in intact nuclei from chicken liver by the use of cis-diammine dichloroplatinum. Methods have been devised for fast purification both of the proteins and of the DNA fragments involved in the cross-linked complexes. By Southern-Western blotting a number of matrix proteins isolated from the complexes have been shown to recognize specifically DNA sequences present in the cross-linked DNA fragments. This experimental approach not only allows to identify the nuclear matrix-DNA interactions existing in the nucleus before its disruption, but also provides a preparation of matrix proteins enriched in those species which are involved in such interactions and which can therefore be detected with high sensitivity.
authors.affiliation: Department of Biochemical Sciences A. Rossi Fanelli, University La Sapienza, Rome, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Ferraro
authors.forename: M
authors.initials: M
authors.lastname: Eufemi
authors.forename: L
authors.initials: L
authors.lastname: Cervoni
authors.forename: F
authors.initials: F
authors.lastname: Altieri
authors.forename: C
authors.initials: C
authors.lastname: Turano
mesh_terms: D000818:Animals; D034961:Antigens, Nuclear; D002467:Cell Nucleus / Q000187:drug effects* / Q000378:metabolism; D002645:Chickens; D002945:Cisplatin; D003432:Cross-Linking Reagents; D004268:DNA-Binding Proteins / Q000378:metabolism*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D009687:Nuclear Proteins / Q000378:metabolism*
chemical_list: D034961:Antigens, Nuclear; D003432:Cross-Linking Reagents; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D002945:Cisplatin