title: High-performance liquid chromatographic determination of spectinomycin in swine, calf and chicken plasma using post-column derivatization.
abstract: An HPLC method for the determination of spectinomycin in swine, calf and chicken plasma at 0.1 microgram/ml or higher is described. The clean-up is based upon ion-pair solid-phase extraction on a High Hydrophobic C18 column treated with sodium dioctyl sulfosuccinate. After elution with methanol, spectinomycin is chromatographed on a Spherisorb SCX column using 0.1 M sodium sulphate solution (pH 2.6)-acetonitrile (80:20, v/v) as mobile phase. Fluorescence detection is at an excitation wavelength of 340 nm and an emission wavelength of 460 nm after post-column oxidation with sodium hypochlorite followed by derivatization with o-phthaldialdehyde. Mean recoveries were 99 +/- 2% (n = 6), 99 +/- 2% (n = 7) and 104 +/- 2% (n = 6) for swine, calf and chicken plasma, respectively, at the 0.1 microgram/ml level.
authors.affiliation: Department of the Science of Food of Animal Origin, Faculty of Veterinary Medicine, University of Utrecht, Netherlands.
authors.forename: N
authors.initials: N
authors.lastname: Haagsma
authors.forename: P
authors.initials: P
authors.lastname: Scherpenisse
authors.forename: R J
authors.initials: RJ
authors.lastname: Simmonds
authors.forename: S A
authors.initials: SA
authors.lastname: Wood
authors.forename: S A
authors.initials: SA
authors.lastname: Rees
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000097:blood*; D002417:Cattle / Q000097:blood*; D002645:Chickens / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D004143:Dioctyl Sulfosuccinic Acid; D007202:Indicators and Reagents; D000198:Spectinomycin / Q000097:blood*; D013050:Spectrometry, Fluorescence; D013552:Swine / Q000097:blood*
chemical_list: D000900:Anti-Bacterial Agents; D007202:Indicators and Reagents; D004143:Dioctyl Sulfosuccinic Acid; D000198:Spectinomycin