PCR-SSCP analysis of the angiotensin II type 1 receptor gene in patients with aldosterone-producing adenomas.
1. In patients with primary aldosteronism due to angiotensin-responsive and angiotensin-unresponsive aldosterone-producing adenomas, no differences in the coding region of the angiotensin II type 1 (AT1) receptor gene were observed compared to normal subjects in peripheral blood leucocyte DNA. 2. Furthermore, no differences in the AT1 receptor gene were observed in DNA extracted from tumour tissue of either subgroup. 3. Genotypic and allelic frequencies for an RFLP detected in the coding region of the AT1 receptor gene were not significantly different between normal subjects and patients with aldosterone-producing adenomas as a group, nor between normal subjects and patients of either subgroup when compared with each other. 4. In those patients heterozygous in peripheral blood at the RFLP site, tumour DNA showed the same allelic pattern. 5. In patients with aldosterone-producing adenomas either responsive or unresponsive to the renin-angiotensin system, no differences were detected using SSCP analysis in the coding region of the AT1 receptor gene in peripheral blood or tumour tissue.
Klemm
S A
SA
Hypertension Unit, Greenslopes Hospital, Brisbane, Queensland, Australia.
Ballantine
D M
DM
Tunny
T J
TJ
Stowasser
M
M
Gordon
R D
RD
D000236:Adenoma / Q000150:complications / Q000235:genetics* / Q000378:metabolism; D000310:Adrenal Gland Neoplasms / Q000150:complications / Q000235:genetics* / Q000378:metabolism; D000328:Adult; D000368:Aged; D000450:Aldosterone / Q000096:biosynthesis*; D000483:Alleles; D001345:Autoradiography; D016009:Chi-Square Distribution; D004247:DNA / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005787:Gene Frequency; D006801:Humans; D006929:Hyperaldosteronism / Q000209:etiology / Q000235:genetics* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D018807:Polymorphism, Single-Stranded Conformational; D011945:Receptors, Angiotensin / Q000235:genetics*
D011945:Receptors, Angiotensin; D000450:Aldosterone; D004247:DNA