title: AF6 gene on chromosome band 6q27 maps distal to the minimal region of deletion in epithelial ovarian cancer.
abstract: Chromosome 6 has been shown to contain at band q27 a minimal region of deletion associated with epithelial ovarian cancers and AF6, a gene disrupted in acute myeloid leukemia with t(6;11)(q27;q23). Using rapid amplification of cDNA ends by polymerase chain reaction, the breakpoint in AF6 was confirmed and a cDNA clone identified. This clone was used as a probe to screen a chromosome 6 cosmid library, and a single cosmid C-109F0645 was isolated. By fluorescence in situ hybridization, C-109F0465 was found to map distal to the critically deleted region associated with ovarian malignancies. AF6 is therefore distinct from and lies telomeric to this region.
authors.affiliation: Department of Medical Oncology, St. Bartholomew's Hospital, London, United Kingdom.
authors.forename: V
authors.initials: V
authors.lastname: Saha
authors.forename: D M
authors.initials: DM
authors.lastname: Lillington
authors.forename: A N
authors.initials: AN
authors.lastname: Shelling
authors.forename: T
authors.initials: T
authors.lastname: Chaplin
authors.forename: M L
authors.initials: ML
authors.lastname: Yaspo
authors.forename: T S
authors.initials: TS
authors.lastname: Ganesan
authors.forename: B D
authors.initials: BD
authors.lastname: Young
mesh_terms: D000208:Acute Disease; D002874:Chromosome Mapping; D002880:Chromosomes, Human, Pair 11 / Q000235:genetics / Q000648:ultrastructure; D002896:Chromosomes, Human, Pair 6 / Q000235:genetics* / Q000648:ultrastructure; D003360:Cosmids / Q000235:genetics; D018284:Cystadenocarcinoma, Serous / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics; D004273:DNA, Neoplasm / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics; D005260:Female; D016147:Genes, Tumor Suppressor; D011495:Histone-Lysine N-Methyltransferase; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D016547:Kinesins / Q000235:genetics*; D007951:Leukemia, Myeloid / Q000235:genetics; D051788:Myeloid-Lymphoid Leukemia Protein; D009218:Myosins / Q000235:genetics*; D015514:Oncogene Proteins, Fusion / Q000235:genetics; D010051:Ovarian Neoplasms / Q000235:genetics*; D011519:Proto-Oncogenes; D017384:Sequence Deletion; D014157:Transcription Factors; D014178:Translocation, Genetic
chemical_list: C095516:AFDN protein, human; D018076:DNA, Complementary; D004273:DNA, Neoplasm; D004268:DNA-Binding Proteins; C497422:KMT2A protein, human; D015514:Oncogene Proteins, Fusion; D014157:Transcription Factors; D051788:Myeloid-Lymphoid Leukemia Protein; D011495:Histone-Lysine N-Methyltransferase; D009218:Myosins; D016547:Kinesins