HLA-DQ beta 1 typing and non-Asp57 alleles in the aborigine population of Senegal.
OBJECTIVE
To investigate, in Senegalese subjects, the frequency of human leukocyte antigen (HLA)-DQ beta 1 alleles and their role in susceptibility to insulin-dependent diabetes mellitus (IDDM).


METHODS
HLA-DQ beta 1 typing was done in 55 IDDM subjects and 118 nondiabetic control subjects by means of polymerase chain reaction restriction fragment length polymorphism.


RESULTS
Alleles bearing a codon for an Asp residue at position 57 in the DQ beta-chain were associated with a significantly lower risk of IDDM. Alleles 0201 and 0302 (Ala57) were positively associated with diabetes, but allele 0501 (Val57) was less frequent in IDDM subjects than in control subjects.


CONCLUSIONS
HLA-DQ beta 1 alleles may be genetic susceptibility markers for IDDM in the Senegalese population, as they are in Caucasian populations.
Chauffert
M
M
Laboratoire de Biochimie, Foundation Hôpital Saint-Joseph, Paris, France.
Cisse
A
A
Chevenne
D
D
Parfait
B
B
Michel
S
S
Trivin
F
F
D000293:Adolescent; D000328:Adult; D000483:Alleles; D001224:Aspartic Acid; D044383:Black People / Q000235:genetics; D002648:Child; D003922:Diabetes Mellitus, Type 1 / Q000453:epidemiology / Q000235:genetics* / Q000276:immunology; D005260:Female; D005787:Gene Frequency; D006580:Genetic Carrier Screening; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006683:HLA-DQ Antigens / Q000235:genetics*; D059866:HLA-DQ beta-Chains; D006650:Histocompatibility Testing; D006720:Homozygote; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D044469:Racial Groups; D012016:Reference Values; D012307:Risk Factors; D012675:Senegal; D014633:Valine
D006683:HLA-DQ Antigens; D059866:HLA-DQ beta-Chains; C069051:HLA-DQB1 antigen; D001224:Aspartic Acid; D014633:Valine