[Diagnosis of bovine parvoviruses in feces of calves].
A haemagglutination assay was tested to detect bovine parvoviruses (BPV) in 456 faecal samples of calves. The assay was not suitable because of a high number of nonspecific haemagglutinating reactions and many haemolytical specimens. A 16fold higher sensitivity was obtained using solid-phase immune electronmicroscopy (SPIEM) in comparison with direct negative contrast staining for detection of BPV. Therefore we examined 117 faecal specimens from young calves of dairy herds from the eastern part of Thuringia by SPIEM. We detected BPV in 2 faecal specimens of calves with diarrhoea and in 3 faecal specimens of clinically normal calves. In comparison with results of other investigators this detection rate of BPV was relatively high.
Elschner
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Bundesinstitut für gesundheitlichen Verbraucherschutz und Veterinärmedizin, Fachbereich 4 Bakterielle Tierseuchen und Bekämpfung von Zoonosen, Jena.
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases; D003967:Diarrhea / Q000175:diagnosis / Q000662:veterinary / Q000821:virology; D005243:Feces / Q000821:virology; D005260:Female; D016253:Microscopy, Immunoelectron; D010322:Parvoviridae Infections / Q000175:diagnosis / Q000662:veterinary*; D017992:Parvovirus / Q000302:isolation & purification; D012680:Sensitivity and Specificity