Interleukin-10 mRNA expression in B-cell chronic lymphocytic leukaemia inversely correlates with progression of disease.
Interleukin-10 (IL-10) has been shown in vitro to inhibit survival and spontaneous DNA synthesis in B-cell chronic lymphocytic leukaemia (B-CELL) cells by induction of programmed cell death. We have analysed the presence of mRNA transcripts for IL-10 in purified B-CLL cells from 35 patients by RT-PCR. Transcripts for IL-10 were detected in 11/20 patients with non-progressive disease. In cell preparations from patients with progressive B-CLL IL-10 mRNA were detected in only 2/15 samples (P < or = 0.01). The Epstein-Barr virus status of the cells did not account for the difference in IL-10 mRNA expression observed between the two groups of patients. Thus, IL-10 mRNA expression in leukaemic cells from patients with B-CLL was strongly associated with non-progressive disease. This finding may support other observations suggesting that IL-10 might be a candidate for immune therapy of progressive B-CLL.
Sjöberg
J
J
Section of Haematology and Immunology, Karolinska Hospital, Stockholm, Sweden.
Aguilar-Santelises
M
M
Sjögren
A M
AM
Pisa
E K
EK
Ljungdahl
A
A
Björkholm
M
M
Jondal
M
M
Mellstedt
H
H
Pisa
P
P
D000368:Aged; D000369:Aged, 80 and over; D017209:Apoptosis / Q000276:immunology; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D018450:Disease Progression; D005260:Female; D006801:Humans; D007167:Immunotherapy; D017403:In Situ Hybridization; D016753:Interleukin-10 / Q000235:genetics* / Q000627:therapeutic use; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000276:immunology* / Q000628:therapy; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011379:Prognosis; D012333:RNA, Messenger / Q000032:analysis*
D017931:DNA Primers; D012333:RNA, Messenger; D016753:Interleukin-10