title: The effects of pH and bile salts on the binding of MeIQx to wheat bran fibre.
abstract: The effects of pH and of the bile salts, sodium cholate, chenodeoxycholate, taurocholate, deoxycholate and glycodeoxycholate on the adsorption of the heterocyclic aromatic amine mutagen and carcinogen 2-amino-3,8-dimethylimidazo[4,5-f]-quinoxaline (MeIQx) onto wheat bran cell wall material have been examined. The highest binding affinity of MeIQx for bran was at pH 5.5. Binding affinity declined more rapidly with pH > 5.5 than with pH < 5.5. Bile salts in solution did not appreciably affect the binding of MeIQx to bran, but where the bile salts formed a suspension then the adsorption was reduced. Where precipitation of bile salts occurred then the free concentration of MeIQx was also reduced, indicating that MeIQx binding/immobilization could be enhanced through interaction with bile salts.
authors.affiliation: Food Molecular Biochemistry Department, Institute of Food Research, Colney, Norwich, UK.
authors.forename: P
authors.initials: P
authors.lastname: Ryden
authors.forename: J A
authors.initials: JA
authors.lastname: Robertson
mesh_terms: D001647:Bile Acids and Salts / Q000494:pharmacology*; D002462:Cell Membrane / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D009153:Mutagens / Q000378:metabolism*; D011804:Quinolines / Q000378:metabolism*; D014908:Triticum / Q000378:metabolism*
chemical_list: D001647:Bile Acids and Salts; D009153:Mutagens; D011804:Quinolines; C036989:2-amino-3,4-dimethylimidazo(4,5-f)quinoline