[The influence of semen preparation and culture medium on the success of IVF in cattle].
The effect of two semen-preparation methods and the presence and absence of capacitation-inducing supplements in the fertilization medium on IVF-results were examined in a 2 x 2 factorial design. In vitro matured oocytes were fertilized either with transmigrated (TM) or with swim-up (SU) prepared native semen. In the first group, the fertilization medium contained 0.8 microgram/ml heparin, 1.2 micrograms/ml hypotaurine and 0.2 microgram/ml epinephrine. However, in the second group, no capacitation-inducing or motility-enhancing substances were used. After SU-treatment, the supplements were necessary to obtain sufficient fertilization results. In the second group (non-supplemented medium), the percentages of penetrated and fertilized oocytes were decreased significantly (P < 0.001). By contrast, transmigrated semen required no additional supplementation of the fertilization medium. The penetration and fertilization rates of this semen-preparation method were equal in both groups.
Rosenkranz
C
C
Institut für Histologie und Embryologie, Veterinarmedizinischen Universität Wien, Osterreich.
Holzmann
A
A
D000818:Animals; D002417:Cattle; D003470:Culture Media / Q000032:analysis / Q000592:standards*; D004837:Epinephrine / Q000032:analysis / Q000494:pharmacology; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods / Q000592:standards / Q000662:veterinary*; D006493:Heparin / Q000032:analysis / Q000494:pharmacology; D008297:Male; D012661:Semen / Q000166:cytology / Q000502:physiology*; D013075:Sperm Capacitation / Q000187:drug effects / Q000502:physiology*; D013081:Sperm Motility / Q000187:drug effects / Q000502:physiology; D013084:Sperm-Ovum Interactions / Q000187:drug effects / Q000502:physiology; D013654:Taurine / Q000031:analogs & derivatives / Q000032:analysis / Q000494:pharmacology
D003470:Culture Media; D013654:Taurine; C003949:hypotaurine; D006493:Heparin; D004837:Epinephrine