Effect of a hematopoietic-promoting factor derived from porcine kidney on megakaryocyte colony formation by murine bone marrow cells.
We investigated the effect of a hematopoietic-promoting factor (HPF) purified from porcine kidney on murine megakaryocyte colony formation. Murine bone marrow cells were cultured in agar in Iscove's modified Dulbecco's medium supplemented with 10% fetal calf serum in the presence of interleukin-3 (IL-3) as a source of megakaryocyte colony-stimulating factor. HPF alone exerted only a slight effect on megakaryocyte colony formation, and the combination of HPF with IL-3 enhanced colony formation compared with IL-3 alone. At days 11 and 13, 1.9-fold and 1.7-fold enhancements were observed, respectively. When erythropoietin (Epo) was added to the combinations of IL-3 plus HPF or IL-3 plus HPF plus SCF megakaryocyte colonies were enhanced compared with the cultures without Epo. As the result of determination the ploidy distribution, more than 20% of the megakaryocytes in IL-3 plus HPF showed 32N and above ploidy, but the number of 8N and under megakaryocytes was the same as those stimulated with IL-3 alone. These results suggest that HPF stimulates both the proliferation of megakaryocyte colony-forming units and the maturation of megakaryocytes in the presence of IL-3.
Kashiwakura
I
I
Department of Radiopharmaceutical Sciences, Hokkaido College of Pharmacy, Otaru, Japan.
Honda
M
M
Hayase
Y
Y
Takagi
Y
Y
D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D000963:Antimetabolites / Q000494:pharmacology; D001792:Blood Platelets / Q000187:drug effects; D001854:Bone Marrow Cells; D004247:DNA / Q000378:metabolism; D004921:Erythropoietin / Q000494:pharmacology; D005472:Fluorouracil / Q000494:pharmacology; D016298:Hematopoietic Cell Growth Factors / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D007377:Interleukin-3 / Q000096:biosynthesis; D015850:Interleukin-6 / Q000494:pharmacology; D007668:Kidney / Q000737:chemistry*; D008297:Male; D008533:Megakaryocytes / Q000187:drug effects*; D051379:Mice; D008815:Mice, Inbred Strains; D011003:Ploidies; D013234:Stem Cells / Q000187:drug effects; D013552:Swine; D014357:Trypsin / Q000494:pharmacology
D000963:Antimetabolites; D016298:Hematopoietic Cell Growth Factors; D007377:Interleukin-3; D015850:Interleukin-6; D004921:Erythropoietin; D004247:DNA; D000110:Acetylcholinesterase; D014357:Trypsin; D005472:Fluorouracil