title: The effect of in vivo and in vitro irradiation (25 Gy) on the subsequent in vitro growth of satellite cells.
abstract: The effect of in vivo and in vitro irradiation on subsequent satellite cell growth, in vitro, was investigated to ascertain the ability of a 25 Gy dose to inhibit satellite cell proliferation. Satellite cells were isolated from the left (irradiated) and right (non-irradiated) Pectoralis thoracicus of two-week-old tom turkeys 16 h (n=3) and seven weeks (n=2) after the left Pectoralis thoracicus had been irradiated (25 Gy). Satellite cells isolated from the irradiated and non-irradiated muscles exhibited similar (P>0.10) in vitro proliferation indicating that a population of satellite cells survived an in vivo dose of 25 Gy. In additional experiments, satellite cell cultures derived from tom turkey Pectoralis thoracicus were irradiated (25 Gy) in vitro. The number of satellite cells did not (P>0.05) increase in irradiated cultures for 134 h following irradiation, while satellite cells in non-irradiated cultures proliferated (P<0.05) over this time. At later time periods, satellite cell number increased (P<0.05) in irradiated cultures indicating that a population of satellite cells survived irradiation. The results of these in vitro experiments suggest that a 25 Gy dose of irradiation does not abolish satellite cell divisions in the turkey Pectoralis thoracicus.
authors.affiliation: Muscle Biology Laboratory, University of Wisconsin, Madison WI 53706, USA.
authors.forename: P E
authors.initials: PE
authors.lastname: Mozdziak
authors.forename: E
authors.initials: E
authors.lastname: Schultz
authors.forename: R G
authors.initials: RG
authors.lastname: Cassens
mesh_terms: D000818:Animals; D002455:Cell Division / Q000528:radiation effects; D002465:Cell Movement; D002470:Cell Survival / Q000528:radiation effects; D002478:Cells, Cultured; D008297:Male; D018482:Muscle, Skeletal / Q000166:cytology / Q000528:radiation effects*; D011833:Radiation Injuries, Experimental / Q000473:pathology*; D014422:Turkeys