title: Failure of the cystic fibrosis transmembrane conductance regulator to conduct ATP.
abstract: The cystic fibrosis transmembrane conductance regulator (CFTR) is a chloride ion channel regulated by protein kinase A and adenosine triphosphate (ATP). Loss of CFTR-mediated chloride ion conductance from the apical plasma membrane of epithelial cells is a primary physiological lesion in cystic fibrosis. CFTR has also been suggested to function an an ATP channel, although the size of the ATP anion is much larger than the estimated size of the CFTR pore. ATP was not conducted through CFTR in intact organs, polarized human lung cell lines, stably transfected mammalian cell lines, or planar lipid bilayers reconstituted with CFTR protein. These findings suggest that ATP permeation through the CFTR is unlikely to contribute to the normal function of CFTR or to the pathogenesis of cystic fibrosis.
authors.affiliation: Division of Biomedical Sciences, University of California, Riverside, 92521, USA.
authors.forename: M M
authors.initials: MM
authors.lastname: Reddy
authors.forename: P M
authors.initials: PM
authors.lastname: Quinton
authors.forename: C
authors.initials: C
authors.lastname: Haws
authors.forename: J J
authors.initials: JJ
authors.lastname: Wine
authors.forename: R
authors.initials: R
authors.lastname: Grygorczyk
authors.forename: J A
authors.initials: JA
authors.lastname: Tabcharani
authors.forename: J W
authors.initials: JW
authors.lastname: Hanrahan
authors.forename: K L
authors.initials: KL
authors.lastname: Gunderson
authors.forename: R R
authors.initials: RR
authors.lastname: Kopito
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D016466:CHO Cells; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D016764:Cell Polarity; D002712:Chlorides / Q000378:metabolism; D006224:Cricetinae; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000378:metabolism*; D006801:Humans; D008051:Lipid Bilayers / Q000378:metabolism; D008168:Lung / Q000166:cytology / Q000378:metabolism; D018408:Patch-Clamp Techniques; D011994:Recombinant Proteins / Q000378:metabolism
chemical_list: C505032:CFTR protein, human; D002712:Chlorides; D008051:Lipid Bilayers; D011994:Recombinant Proteins; D019005:Cystic Fibrosis Transmembrane Conductance Regulator; D000255:Adenosine Triphosphate