title: Primary structure of a cytosolic malate dehydrogenase of the amitochondriate eukaryote, Trichomonas vaginalis.
abstract: The nucleotide sequence of a gene coding for a 37 kDa subunit of a cytosolic malate dehydrogenase of Trichomonas vaginalis was established. The sequence of a gDNA clone and a cDNA clone, which lacked seven amino-terminal codons, were identical, indicating an absence of introns from the gene. Cell fractionation combined with sequencing of peptide fragments of the purified enzyme showed that the gene codes for an expressed cytosolic enzyme. The derived amino acid sequence was closely related to cytosolic malate dehydrogenases from animals and plants and from the eubacteria Thermus aquaticus and Mycobacterium leprae and was more distant from the enzyme of mitochondria and from Escherichia coli and certain other eubacteria. In phylogenetic reconstructions this enzyme shared a most recent common ancestor with other cytosolic enzymes.
authors.affiliation: Rockefeller University, New York, NY 10021, USA.
authors.forename: A
authors.initials: A
authors.lastname: Markos
authors.forename: A
authors.initials: A
authors.lastname: Morris
authors.forename: C
authors.initials: C
authors.lastname: Rozario
authors.forename: M
authors.initials: M
authors.lastname: Müller
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003600:Cytosol / Q000201:enzymology*; D017125:Genes, Protozoan / Q000235:genetics; D008291:Malate Dehydrogenase / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry; D010802:Phylogeny; D017421:Sequence Analysis; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014246:Trichomonas vaginalis / Q000145:classification / Q000201:enzymology* / Q000235:genetics
chemical_list: D010446:Peptide Fragments; D008291:Malate Dehydrogenase