Morpho-functional modifications of human syncytiotrophoblast plasma membrane during pregnancy induced hypertension.
A decrease of Na+/K(+)-ATPase activity has been reported in syncytiotrophoblast plasma membrane (SPM) obtained from pregnancy induced hypertensive (PIH) women. The aim of the present work was to verify if the reported modifications in activity are due to a decreased number of enzymatic molecules or to a conformational change of the enzyme itself. Morphological studies were performed in order to better understand the relations between the enzymatic protein and the lipid bilayer. Kinetic studies were also performed. SPM obtained from PIH showed: i) an increased affinity of Na+/K(+)-ATPase for ouabain binding, ii) a significant change in the maximum velocity of the enzyme, iii) a higher distribution factor (DF) of intramembrane particles (IMPs) in the exoplasmic face of the membrane, iv) a decreased mean diameter of IMPs both in the protoplasmic and exoplasmic faces, v) a decreased number of IMPs in the exoplasmic face. In conclusion, a conformational modification seems to be at the basis of the decreased Na+/K(+)-ATPase activity during PIH as suggested by binding, ultrastructural and kinetic data herein reported.
Biagini
G
G
Instituti di Morfologia Umana Normale e Biochimica, Universita' di Roma, Tor Vergata, Italy.
Salvolini
E
E
Pugnaloni
A
A
Rabini
R A
RA
Cester
N
N
Romanini
C
C
Staffolani
R
R
Mazzanti
L
L
D000328:Adult; D016022:Case-Control Studies; D002462:Cell Membrane / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000378:metabolism; D005260:Female; D005614:Freeze Fracturing; D015726:Giant Cells / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D006973:Hypertension / Q000209:etiology / Q000378:metabolism / Q000473:pathology*; D007700:Kinetics; D010042:Ouabain / Q000378:metabolism; D011247:Pregnancy; D011249:Pregnancy Complications, Cardiovascular / Q000378:metabolism / Q000473:pathology*; D011487:Protein Conformation; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors*; D014327:Trophoblasts / Q000378:metabolism / Q000473:pathology*
D004791:Enzyme Inhibitors; D010042:Ouabain; D000254:Sodium-Potassium-Exchanging ATPase