title: Desulfitobacterium sp. strain PCE1, an anaerobic bacterium that can grow by reductive dechlorination of tetrachloroethene or ortho-chlorinated phenols.
abstract: A strictly anaerobic bacterium, strain PCE1, was isolated from a tetrachloroethene-dechlorinating enrichment culture. Cells of the bacterium were motile curved rods, with approximately four lateral flagella. They possessed a gram-positive type of cell wall and contained cytochrome c. Optimum growth occurred at pH 7.2-7.8 and 34-38 degrees C. The organism grew with L-lactate, pyruvate, butyrate, formate, succinate, or ethanol as electron donors, using either tetrachloroethene, 2-chlorophenol, 2,4,6-trichlorophenol, 3-chloro-4-hydroxy-phenylacetate, sulfite, thiosulfate, or fumarate as electron acceptors. Strain PCE1 also grew fermentatively with pyruvate as the sole substrate. L-Lactate and pyruvate were oxidized to acetate. Tetrachloroethene was reductively dechlorinated to trichloroethene and small amounts ( 5%) of cis-1,2-dichloroethene and trans-1,2-dichloroethene. Chlorinated phenolic compounds were dechlorinated specifically at the ortho-position. On the basis of 16S rRNA sequence analysis, the organism was identified as a species within the genus Desulfitobacterium, which until now only contained the chlorophenol-dechlorinating bacterium, Desulfitobacterium dehalogenans.
authors.affiliation: Department of Microbiology, University of Groningen, Kerklaan 30, 9751 NN Haren, The Netherlands.
authors.forename: J
authors.initials: J
authors.lastname: Gerritse
authors.forename: V
authors.initials: V
authors.lastname: Renard
authors.forename: T M
authors.initials: TM
authors.lastname: Pedro Gomes
authors.forename: P A
authors.initials: PA
authors.lastname: Lawson
authors.forename: M D
authors.initials: MD
authors.lastname: Collins
authors.forename: J C
authors.initials: JC
authors.lastname: Gottschal
mesh_terms: D001421:Bacteria, Anaerobic / Q000166:cytology / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002733:Chlorophenols / Q000378:metabolism*; D003470:Culture Media; D006863:Hydrogen-Ion Concentration; D007773:Lactates / Q000378:metabolism; D019344:Lactic Acid; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D011773:Pyruvates / Q000378:metabolism; D019289:Pyruvic Acid; D012988:Soil Microbiology; D013696:Temperature; D013750:Tetrachloroethylene / Q000378:metabolism*
chemical_list: D002733:Chlorophenols; D003470:Culture Media; D007773:Lactates; D011773:Pyruvates; D019344:Lactic Acid; D019289:Pyruvic Acid; D013750:Tetrachloroethylene