Production and characterisation of anti-cardiac troponin-I monoclonal antibodies.
Cardiac troponin-I (cTn-I) was isolated from bovine left ventricular tissue and used as immunogen. Sixteen murine hybridoma lines were produced with two of them, 1D12 and 5F4, showing a high specificity for cTn-I; both of these monoclonal antibodies (McAbs) were isotyped as IgG1 with kappa-light chains. The specificity of the McAbs for cTn-I was confirmed by ELISA, western blotting and by the ability of the antibodies to block actomyosin ATPase inhibition by cTn-I. The McAbs may be useful for human in vivo imaging of myocardial infarcts and other pathological conditions related to cardiac myocyte damage.
Haider
K H
KH
Faculty of Pharmacy, University of the Punjab, Lahore, Pakistan.
Stimson
W H
WH
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000917:Antibody Formation; D015153:Blotting, Western; D002417:Cattle; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D009206:Myocardium / Q000378:metabolism*; D014336:Troponin / Q000276:immunology* / Q000302:isolation & purification; D019210:Troponin I
D000911:Antibodies, Monoclonal; D014336:Troponin; D019210:Troponin I