Electrorotational studies of the cytoplasmic dielectric properties of Friend murine erythroleukaemia cells.
Electrorotation (ROT) spectra of Friend murine erythroleukaemia DS19 cells were measured in the frequency range 10 kHz-100 MHz as a function of suspension osmolality and cell differentiation treatment with hexamethylene bisacetamide (HMBA). A minimization program was employed to curve-fit the measured spectra using a single-shell dielectric model, allowing the derivation of cellular interior conductivity and permittivity (valid for the frequency range 10-100 MHz) and the cytoplasmic membrane capacitance (its dependence on the cell differentiation state and suspension osmolality having been reported earlier). Following HMBA treatment, DS19 cells exhibited a slight increment in average interior permittivity and a decrement in interior conductivity, although the changes were not statistically significant. For both untreated and HMBA treated samples, the average interior conductivity increased and permittivity decreased with increasing suspension osmolality. Of significance was that the average permittivity of cell interiors was larger than that of pure water. The electrorotation spectra of freshly prepared cell nuclei were measured, and the derived nuclear dielectric parameters were employed in numerical simulations to investigate the effects of nuclei on the ROT spectra of intact cells. Other cellular internal structures such as mitochondria were also analysed using theoretical simulations. It was concluded that the derived large permittivity values did not result from cell nuclei or mitochondria, and, instead, we suggest that they may arise from the combined effects of several cytoplasmic organelles.
Huang
Y
Y
Department of Molecular Pathology, University of Texas M D Anderson Cancer Center, Houston 77030, USA.
Wang
X B
XB
Hölzel
R
R
Becker
F F
FF
Gascoyne
P R
PR
D000818:Animals; D055592:Biophysical Phenomena; D001703:Biophysics; D002451:Cell Compartmentation; D002467:Cell Nucleus / Q000378:metabolism; D004553:Electric Conductivity; D005622:Friend murine leukemia virus; D004915:Leukemia, Erythroblastic, Acute / Q000378:metabolism*; D051379:Mice; D008928:Mitochondria / Q000378:metabolism; D008954:Models, Biological; D012399:Rotation; D014407:Tumor Cells, Cultured