Characterization of the nuclear gene encoding chloroplast ribosomal protein S13 from Arabidopsis thaliana.
We have characterised a cDNA clone and a nuclear gene encoding the chloroplast 30 s ribosomal protein S13 from Arabidopsis thaliana. The identification is based on the high similarity of the predicted amino-acid sequence with eubacterial S13 protein sequences, and immunodetection of a 14.5-kDa chloroplast ribosomal polypeptide using antibodies raised against the polypeptide produced from part of the cDNA expressed in bacteria. The predicted amino-acid sequence contains an N-terminal extension which has several features characteristic of chloroplast transit peptides. Experiments suggest there is a single copy of this gene in A. thaliana and multiple copies in Brassica species. The origin of the mitochondrial S13 polypeptide in crucifers is also discussed.
Kumar
R
R
Station de Génétique et d'Amélioration des Plantes, INRA, Versailles, France.
Drouaud
J
J
Raynal
M
M
Small
I
I
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D015153:Blotting, Western; D002467:Cell Nucleus / Q000737:chemistry / Q000235:genetics; D002736:Chloroplasts / Q000737:chemistry*; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D018076:DNA, Complementary; D019143:Evolution, Molecular; D017343:Genes, Plant; D008928:Mitochondria / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010802:Phylogeny; D012269:Ribosomal Proteins / Q000032:analysis / Q000737:chemistry*; D016415:Sequence Alignment; D017421:Sequence Analysis
D018076:DNA, Complementary; D012269:Ribosomal Proteins