Determination of 1,2,6-inositol trisphosphate (derivatives) in plasma using iron(III)-loaded adsorbents and capillary zone electrophoresis-(indirect) UV detection.
A method for the determination of 1,2,6-inositol trisphosphate (IP3) and derivatives in plasma by capillary zone electrophoresis with (indirect) UV detection has been developed. The sample pretreatment is based on the selective isolation after complexation of inositol phosphates with iron(III) loaded on an adsorbent. Plasma protein denaturation was performed with sodium dodecyl sulfate. The selectivity of the method is demonstrated with the analysis of phenylacetate-IP3. The recoveries amount to 65% and 88% in plasma and in water, respectively.
Buscher
B A
BA
Division of Analytical Chemistry, Leiden/Amsterdam Center for Drug Research, University of Leiden, Netherlands.
Tjaden
U R
UR
Irth
H
H
Andersson
E M
EM
van der Greef
J
J
D000327:Adsorption; D019075:Electrophoresis, Capillary / Q000379:methods*; D005290:Ferric Compounds / Q000737:chemistry*; D006801:Humans; D007295:Inositol Phosphates / Q000032:analysis / Q000097:blood*; D010648:Phenylacetates / Q000032:analysis / Q000097:blood*; D011489:Protein Denaturation; D012822:Silicon Dioxide / Q000737:chemistry; D012967:Sodium Dodecyl Sulfate; D013056:Spectrophotometry, Ultraviolet
D005290:Ferric Compounds; D007295:Inositol Phosphates; D010648:Phenylacetates; D012967:Sodium Dodecyl Sulfate; D012822:Silicon Dioxide; C059368:atrinositol