Four frequently used test systems for serum cholesterol evaluated by isotope dilution gas chromatography-mass spectrometry candidate reference method.
We evaluated the performance of four frequently used cholesterol test systems, using split-sample measurements with a panel of 79 patients' specimens and isotope dilution gas chromatography-mass spectrometry (ID GS-MS) as a comparison method. The test systems were from Beckman, a Boehringer Mannheim, Merck, and Johnson & Johnson Clinical Diagnostics, performed on the Synchron CX7, Hitachi 717, Mega, and Ektachem 250 analyzers, respectively. The liner regression data for the method comparison [ID GS-MS as independent variable (x)] were for Beckman: slope = 1.012, intercept = 0.0243 mmol/L, dispersion (S(y/x)) = 0.1303 mmol/l, and correlation coefficient (r) = 0.9867; for Boehringer Mannheim: slope = 1.002, intercept = 0.114 mmol/L, Sy/x = 0.0759 mmol/L, r = 0.9954; for Merck: slope = 1.034, intercept = -0.0613 mmol/L, Sy/x = 0.0886 mmol/L, r = 0.9941; and for Johnson & Johnson Clinical Diagnostics: slope = 1.007, intercept = 0.01 mmol/L, Sy/x = 0.15 mmol/L, and r = 0.9811. These data demonstrate excellent state-of-the-art cholesterol measurement for some of the most widely used test systems.
Thienpont
L M
LM
Laboratoria voor Medische Biochemie en voor Klinische Analyse, University of Ghent, Belgium.
Van Landuyt
K G
KG
Stöckl
D
D
De Leenheer
A P
AP
D001774:Blood Chemical Analysis / Q000379:methods / Q000592:standards* / Q000706:statistics & numerical data; D002784:Cholesterol / Q000097:blood*; D008401:Gas Chromatography-Mass Spectrometry / Q000706:statistics & numerical data; D006801:Humans; D007201:Indicator Dilution Techniques; D011786:Quality Control
D002784:Cholesterol