Effect of insect-growth-disrupting amines and amides on Schistosoma mansoni in vitro.
A series of nonsteroidal alkylamines and alkylamides, which apparently inhibit phytosterol dealkylation in insects and free-living nematodes, as well as steps in the ecdysteroid biosynthetic pathway of insects, are also known to affect growth and development of animal-parasitic nematodes. The effect of these compounds on the motility, pairing, and viability of the trematode, Schistosoma mansoni, maintained in vitro was examined by incorporating the potential inhibitors into the medium employed for parasite culture. The alkylamines were more active than their amide counterparts, killing all parasites within 18 hr when tested at 25 parts per million. At lower concentrations, reductions in schistosome pairing and motility were observed. The functional group of the amine compounds influenced the schistosomicidal activity, with N-ethylamines being more effective than N,N-dimethylamines of identical carbon chain-length. Of all the compounds tested, N-ethyldodecanamine appeared to be the most promising schistosomicidal agent, killing all parasites within 22 hr at 10 parts per million and preventing parasite pairing at 3 parts per million.
Foster
J M
JM
Department of Biochemistry, University of Liverpool, UK.
Marshall
I
I
Svoboda
J A
JA
Thompson
M J
MJ
Rees
H H
HH
D000577:Amides / Q000494:pharmacology*; D000588:Amines / Q000494:pharmacology*; D000818:Animals; D026461:Ecdysteroids; D005260:Female; D007301:Insect Hormones / Q000378:metabolism; D007313:Insecta / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D008297:Male; D012550:Schistosoma mansoni / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D012727:Sex Characteristics; D013256:Steroids / Q000378:metabolism
D000577:Amides; D000588:Amines; D026461:Ecdysteroids; D007301:Insect Hormones; D013256:Steroids