title: Genetic structure of Neisseria meningitidis serogroup C epidemic strains in south Brazil.
abstract: In the present study we report the results of an analysis, based on serotyping, multilocus enzyme electrophoresis (MEE), and ribotyping of N. meningitidis serogroup C strains isolated from patients with meningococcal disease (MD) in Rio Grande do Sul (RS) and Santa Catarina (SC) States, Brazil, as the Center of Epidemiology Control of Ministry of Health detected an increasing of MD cases due to this serogroup in the last two years (1992-1993). We have demonstrated that the MD due to N.meningitidis serogroup C strains in RS and SC States occurring in the last 4 years were caused mainly by one clone of strains (ET 40), with isolates indistinguishable by serogroup, serotype, subtype and even by ribotyping. One small number of cases that were not due to an ET 40 strains, represent closely related clones that probably are new lineages generated from the ET 40 clone referred as ET 11A complex. We have also analyzed N.meningitidis serogroup C strains isolated in the greater São Paulo in 1976 as representative of the first post epidemic year in that region. The ribotyping method, as well as MEE, could provide useful information about the clonal characteristics of those isolates and also of strains isolated in south Brazil. The strains from 1976 have more similarity with the actual endemic than epidemic strains, by the ribotyping, sulfonamide sensitivity, and MEE results. In conclusion, serotyping with monoclonal antibodies (C:2b:P1.3), MEE (ET 11 and ET 11A complex), and ribotyping by using ClaI restriction enzyme (Rb2), were useful to characterize these epidemic strains of N.meningitidis related to the increased incidence of MD in different States of south Brazil. It is mostly probable that these N.meningitidis serogroup C strains have poor or no genetic correlation with 1971-1975 epidemic serogroup C strains. The genetic similarity of members of the ET 11 and ET 11A complex were confirmed by the ribotyping method by using three restriction endonucleases.
authors.affiliation: Bacteriology Division, Adolfo Lutz Institute, São Paulo, Brazil. sacchi@usp.br
authors.forename: C T
authors.initials: CT
authors.lastname: Sacchi
authors.forename: M L
authors.initials: ML
authors.lastname: Tondella
authors.forename: M C
authors.initials: MC
authors.lastname: Gorla
authors.forename: P S
authors.initials: PS
authors.lastname: de Lemos
authors.forename: C E
authors.initials: CE
authors.lastname: Melles
authors.forename: M V
authors.initials: MV
authors.lastname: de Paiva
authors.forename: D S
authors.initials: DS
authors.lastname: Rodrigues
authors.forename: A J
authors.initials: AJ
authors.lastname: Andrade
authors.forename: M O
authors.initials: MO
authors.lastname: Ribeiro
authors.forename: A
authors.initials: A
authors.lastname: Sperb
mesh_terms: D015373:Bacterial Typing Techniques; D001938:Brazil / Q000453:epidemiology; D006801:Humans; D008589:Meningococcal Infections / Q000453:epidemiology*; D009345:Neisseria meningitidis / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification