Effect of pharmacological agents on the productions of interleukin-10 by the murine D10.G4.1 TH2 cell line in vitro.
It is becoming increasingly clear that the clinical courses of a variety of autoimmune and infectious diseases are influenced by the balance of TH1 and TH2 cell subsets that are generated during the immune response. IL-10 is one of several cytokines which influences the differentiation of TH cell subsets and represents a target for therapeutic intervention. We have evaluated a variety of pharmacological agents for their ability to modulate IL-10 release by the murine D10.G4.1 TH2 cell line when stimulated with concanacavalin-A in the presence of IL-1 alpha. Several were inhibitory, and the concentrations which caused a 50% reduction in IL-10 production were 0.38 microM cyclosporin-A, 0.0073 microM dexamethasone, 0.045 microM prednisolone and 0.31 microM cycloheximide. Methotrexate and pentoxifyline caused a weak but statistically significant reduction in IL-10 production at a concentration of 10 microM (P < or = 0.05), whereas amrinone and azathioprine had no clear effect. The pharmacological agents tested are known to exert multiple effects and were evaluated with a view to their use as reference standards in an ongoing screening programme to identify novel compounds which specifically modulate Il-10 production.
Tomkins
P T
PT
Boots Pharmaceuticals Research Department, Nottingham, U.K.
Cooper
K L
KL
Appleby
P
P
Webber
D G
DG
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000494:pharmacology; D002460:Cell Line; D003208:Concanavalin A / Q000494:pharmacology; D007166:Immunosuppressive Agents / Q000494:pharmacology; D007375:Interleukin-1 / Q000502:physiology; D016753:Interleukin-10 / Q000096:biosynthesis*; D051379:Mice; D011994:Recombinant Proteins / Q000494:pharmacology; D013256:Steroids; D013601:T-Lymphocytes / Q000187:drug effects / Q000378:metabolism*
D000893:Anti-Inflammatory Agents; D000970:Antineoplastic Agents; D007166:Immunosuppressive Agents; D007375:Interleukin-1; D011994:Recombinant Proteins; D013256:Steroids; D003208:Concanavalin A; D016753:Interleukin-10