Ultrastructural cytochemistry of human gastric cancer: electron microscopic observations of five organellae marker enzymes.
The distribution of ALPase, ACPase, G6Pase TPPase and CCOase of gastric cancer and normal gastric epithelium were studied ultrastructurally. The results showed that normal gastric epithelium had no ALPase reaction. The reactions of ACPase, G6Pase, TPPase and CCOase were found in the corresponding organellae which were consistent with their functions. In tubular adenocarcinoma cells, their reactions were more apparent in the corresponding organellae. Some cells of tubular adenocarcinomas showed ALPase reaction. The mucinous adenocarcinoma cells had higher ACPase and TPPase reactions. In poorly differentiated adenocarcinoma cells, the five marker enzymes showed negative or faint reactions. The biological significance and mechanisms of distribution of the five marker enzymes were discussed.
Yang
G
G
Department of Pathology, Anhui Medical University, Hefei.
Dong
Y
Y
Du
W
W
Su
Y
Y
Zhang
H
H
Wu
J
J
Wang
D
D
Xu
A
A
D000135:Acid Phosphatase / Q000032:analysis*; D000230:Adenocarcinoma / Q000201:enzymology / Q000648:ultrastructure; D002288:Adenocarcinoma, Mucinous / Q000201:enzymology / Q000648:ultrastructure; D000469:Alkaline Phosphatase / Q000032:analysis*; D014408:Biomarkers, Tumor / Q000032:analysis*; D005952:Glucose-6-Phosphatase / Q000032:analysis*; D006651:Histocytochemistry; D006801:Humans; D013274:Stomach Neoplasms / Q000201:enzymology* / Q000648:ultrastructure
D014408:Biomarkers, Tumor; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase; D005952:Glucose-6-Phosphatase