Novel features of drosophila period Transcription revealed by real-time luciferase reporting.
The rapid turnover of luciferase and the sensitive, non-invasive nature of its assay make this reporter gene uniquely situated for temporal gene expression studies. To determine the in vivo regulatory pattern of the Drosophila clock gene period (per), we generated transgenic strains carrying a luciferase cDNA fused to the promoter region of the per gene. This has allowed us to monitor circadian rhythms of bioluminescence from pacemaker cells within the head for several days in individual living adults. These high time-resolution experiments permitted neuronal per transcription and opens the door to vastly simplified experiments in general chronobiology and studies of temporally regulated transcription in a wide range of experimental systems.
Brandes
C
C
Department of Biology, Brandeis University, Waltham, Massachusetts 02254 USA.
Plautz
J D
JD
Stanewsky
R
R
Jamison
C F
CF
Straume
M
M
Wood
K V
KV
Kay
S A
SA
Hall
J C
JC
D000818:Animals; D030801:Animals, Genetically Modified; D002940:Circadian Rhythm; D018076:DNA, Complementary; D004330:Drosophila / Q000235:genetics*; D029721:Drosophila Proteins; D017930:Genes, Reporter; D008156:Luciferases / Q000235:genetics* / Q000378:metabolism; D008163:Luminescent Measurements; D009474:Neurons / Q000378:metabolism*; D009687:Nuclear Proteins / Q000235:genetics*; D056950:Period Circadian Proteins; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000378:metabolism; D011993:Recombinant Fusion Proteins; D014158:Transcription, Genetic
D018076:DNA, Complementary; D029721:Drosophila Proteins; D009687:Nuclear Proteins; C539040:PER protein, Drosophila; D056950:Period Circadian Proteins; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D008156:Luciferases