Reperfusion hastens appearance and extent of distribution of type I collagen in infarct zone: immunohistochemical study in rat experimental infarction.
BACKGROUND
The effects of reperfusion on time-dependent alteration of type I collagen have not been examined.


OBJECTIVE
We compared the sequential changes in the appearance and distribution of type I collagen in reperfused infarct rat hearts to those in non-reperfused hearts.


METHODS
Using an experimental rat model of infarction, we performed immunohistochemical staining with a polyclonal antibody to type I collagen by the avidin-biotin-peroxidase method. Reperfusion was established after 2-h coronary ligation that produced complete necrosis of myocytes.


RESULTS
In reperfused hearts, type I collagen appeared in the peripheral zone of the infarct at day 2, which was 1 day earlier than in non-reperfused hearts. The extent of distribution of type I collagen in reperfused hearts was comparable to that observed approximately 1 day later in non-reperfused hearts.


CONCLUSIONS
Reperfusion can accelerate collagen matrix formation compared with that in non-reperfused hearts after acute myocardial infarction.
Yamasaki
S
S
First Department of Internal Medicine, Okayama University Medical School, Japan.
Kusachi
S
S
Moritani
H
H
Kondo
J
J
Hirohata
S
S
Tamura
A
A
Tsuji
T
T
D000818:Animals; D003094:Collagen / Q000032:analysis / Q000378:metabolism*; D005109:Extracellular Matrix / Q000378:metabolism; D007124:Immunoenzyme Techniques; D008297:Male; D009203:Myocardial Infarction / Q000378:metabolism*; D015425:Myocardial Reperfusion / Q000009:adverse effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
D003094:Collagen