Effect of temperature on spontaneous Ca2+-activated Cl- currents in rabbit portal vein cells.
The effect of temperature on the time course of spontaneous transient inward (Ca2+-activated Cl-) currents (STICs) was investigated with the perforated patch technique in rabbit portal vein cells. STICs decayed exponentially at temperatures between 17 degrees C and 35 degrees C. The time constant of the decay (tau ) was decreased with increasing temperature and had a temperature coefficient, Q10, of 2.6. The temperature sensitivity of tau could be described by the Arrhenius equation which gave an activation energy of 16kcal/mol. This result indicates that STIC decay is governed by a single first-order reaction and provides further support for the hypothesis that the decay is due to the gating properties of Ca2+-activated Cl- channels.
Helliwell
R M
RM
Department of Pharmacology and Clinical Pharmacology, St. George s Hospital Medical School, Cranmer Terrace, London SW17 0RE, UK.
Large
W A
WA
D000818:Animals; D002118:Calcium / Q000502:physiology*; D002462:Cell Membrane / Q000502:physiology; D018118:Chloride Channels / Q000378:metabolism*; D004594:Electrophysiology; D066298:In Vitro Techniques; D008564:Membrane Potentials / Q000502:physiology; D009131:Muscle, Smooth, Vascular / Q000378:metabolism*; D018408:Patch-Clamp Techniques; D011169:Portal Vein / Q000378:metabolism*; D011817:Rabbits; D013696:Temperature
D018118:Chloride Channels; D002118:Calcium