Molecular cloning, cDNA structure, and chromosomal localization of the human type II cGMP-dependent protein kinase.
The type II cGMP-dependent protein kinase is an enzyme originally isolated from the small intestine, and is thought to be involved in the regulation of intestinal ion transport and fluid secretion. A complementary DNA clone encoding a part of the human type II cGMP-dependent protein kinase was isolated from a cerebellum library. Based on sequence information from this complementary DNA, the 5'-end of the type II cGMP-dependent protein kinase was amplified from human brain messenger RNA using polymerase chain reaction. The composite complementary DNA encoded a 762 amino acid protein with a calculated molecular mass of 87.4 kDa. Messenger RNAs encoding the type II cGMP-dependent protein kinase were detected in small intestine, colon and prostate. By using polymerase chain reaction and Southern blotting on somatic cell hybrids, the gene encoding, the type II cGMP-dependent protein kinase was mapped to human chromosome 4q13.1-q21.1.
Orstavik
S
S
Institute of Medical Biochemistry, Faculty of Medicine, University of Oslo, Norway.
Solberg
R
R
Taskén
K
K
Nordahl
M
M
Altherr
M R
MR
Hansson
V
V
Jahnsen
T
T
Sandberg
M
M
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002531:Cerebellum / Q000201:enzymology*; D002874:Chromosome Mapping; D002894:Chromosomes, Human, Pair 4; D003001:Cloning, Molecular; D003106:Colon / Q000201:enzymology; D017869:Cyclic GMP-Dependent Protein Kinases / Q000096:biosynthesis* / Q000235:genetics*; D017931:DNA Primers; D018076:DNA, Complementary; D005260:Female; D015723:Gene Library; D006801:Humans; D006822:Hybrid Cells; D007421:Intestine, Small / Q000201:enzymology; D007527:Isoenzymes / Q000096:biosynthesis / Q000235:genetics; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010053:Ovary / Q000201:enzymology; D016133:Polymerase Chain Reaction; D011467:Prostate / Q000201:enzymology; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis
D017931:DNA Primers; D018076:DNA, Complementary; D007527:Isoenzymes; D012333:RNA, Messenger; D017869:Cyclic GMP-Dependent Protein Kinases