Relation of some enzyme activities and argyrophilic proteins in childhood acute lymphoblastic leukemia.
In a series of 61 children with newly diagnosed acute lymphoblastic leukemia (ALL) the detection of argyrophilic proteins (AgNORs) in relation to enzymatic profile of leukemic blasts was undertaken. The method of silver staining was used to determine the number of AgNORs per nucleus of cells. The activity of 5'nucleotidase, acid phosphatase and beta-glucuronidase was assessed. The AgNOR proteins quantity varied with immunophenotype and cytochemical profile of leukemic cells. The enzyme 5'nucleotidase is known to be the marker enzyme in beta-precursors ALL and acid phosphatase in T-ALL blast cells. Activity of beta-glucuronidase emerged in lymphoblasts of some cases of ALL in close relation to increased number of AgNOR proteins per nucleus of leukemic cells. Our study indicates the possible importance of beta-glucuronidase involvement and increase AgNOR quantity in the proliferative activity of leukemic cells and thus they are of value in monitoring the risk groups of leukemic patients.
Klobusická
M
M
Cancer Research Institute, Slovak Academy of Sciences, Bratislava, Slovakia.
Babusíková
O
O
Mesárosová
A
A
Cáp
J
J
D000293:Adolescent; D034961:Antigens, Nuclear; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D016130:Immunophenotyping; D007223:Infant; D008297:Male; D009687:Nuclear Proteins / Q000032:analysis*; D009697:Nucleolus Organizer Region / Q000737:chemistry*; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000201:enzymology* / Q000276:immunology / Q000401:mortality; D011379:Prognosis
D034961:Antigens, Nuclear; D009687:Nuclear Proteins; C051963:nucleolar organizer region associated proteins