Isolation of strong promoters from Clavibacter xyli subsp. cynodontis using a promoter probe plasmid.
To isolate promoters from Clavibacter xyli subsp. cynodontis (C. xyli subsp. cynodontis), we constructed a new promoter probe plasmid and made a C. xyli subsp. cynodontis promoter probe library. Two promoters gave over 2500-times stronger expression than the parental plasmid. The promoters were sequenced and compared to other bacterial promoters. These C. xyli subsp. cynodontis promoter regions are GC-rich and do not resemble E. coli promoters, but do resemble a few individual promoters found in streptomycetes.
Haapalainen
M
M
Department of Biology, University of Turku, Finland.
Karp
M
M
Metzler
M C
MC
D000192:Actinomycetales / Q000235:genetics*; D001483:Base Sequence; D016384:Consensus Sequence; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification*; D004926:Escherichia coli / Q000235:genetics; D006094:Gram-Positive Bacteria / Q000235:genetics; D008969:Molecular Sequence Data; D010957:Plasmids; D011401:Promoter Regions, Genetic; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D013302:Streptomyces / Q000235:genetics; D014169:Transformation, Bacterial
D004269:DNA, Bacterial