title: Multicentre evaluation of Capture Assay Radim Liquid Allergen for measurement of specific IgE antibodies.
abstract: A multicentre trial of Capture Assay Radim Liquid Allergen was performed to define the sensitivity, specificity and clinical reliability of the system in diagnostic allergology. The results of the evaluation were compared with clinical data and in vivo testing. Good agreement was obtained for Dermatophagoides pteronyssinus (D1), Cat's epithelium (E1), Betula verrucosa (T3) and Olea europea (T9), Artemisia vulgaris (W6) and Parietaria officinalis (W19). Some spreading of data was observed for Artemisia absinthium (W5), Cynodon dactylon (G2), and Lolium perenne (G5). We found a high number of negative cases for Alternaria alternata (M6). The advantages offered by the system are the automation, the small quantity of serum requested, the supply of quantitative results in international units of specific IgE, the user-friendly software. The data are sufficiently reliable for the diagnostic system to be introduced into the clinical laboratory allergological routine.
authors.affiliation: Dipartimento di Medicina di Laboratorio, Istituto Scientifico, H San Raffaele, Milano, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Banfi
authors.forename: F
authors.initials: F
authors.lastname: Ferrara
authors.forename: P
authors.initials: P
authors.lastname: Bonini
authors.forename: G
authors.initials: G
authors.lastname: Colombo
authors.forename: M
authors.initials: M
authors.lastname: Plebani
authors.forename: F
authors.initials: F
authors.lastname: Borghesan
authors.forename: D
authors.initials: D
authors.lastname: Faggian
authors.forename: M
authors.initials: M
authors.lastname: Caputo
authors.forename: L
authors.initials: L
authors.lastname: Andri
authors.forename: G E
authors.initials: GE
authors.lastname: Senna
mesh_terms: D000293:Adolescent; D000328:Adult; D000485:Allergens / Q000032:analysis / Q000276:immunology*; D000918:Antibody Specificity; D002648:Child; D002675:Child, Preschool; D005069:Evaluation Studies as Topic; D006801:Humans; D006967:Hypersensitivity / Q000097:blood / Q000175:diagnosis; D007073:Immunoglobulin E / Q000097:blood* / Q000276:immunology; D008875:Middle Aged; D011852:Radioallergosorbent Test; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012882:Skin Tests
chemical_list: D000485:Allergens; D007073:Immunoglobulin E