title: Determination of DDT and related compounds in blood samples from agricultural workers.
abstract: An analytical method combining a solid-phase (C18) clean-up and GC-electron-capture detection using a capillary column, was implemented to determine p,p'-DDT and its metabolites (p,p'-DDD and p,p'-DDE), as well as other organochlorine pesticides in whole blood samples from 30 farmers and 24 non-occupationally exposed workers. The average concentrations for the quantified pesticides, p,p'-DDT, p,p'-DDD and p,p'-DDE, were 0.9, 1.5 and 8.0 micrograms/l whole blood for exposed workers and 0.3, 0.5 and 3.3 micrograms/l for unexposed workers, respectively. GC-MS was used to confirm the identity of the pesticides found. Solid-phase extraction and the protocol used give a cleaner analytical matrix, not only improving sensitivity and resolution, but also allowing analyses with smaller blood samples as compared to other methods.
authors.affiliation: Instito Nacional de Seguridad e Higiene en el Trabajo, Centro Nacional de Condiciones de Trabajo, Barcelona, Spain.
authors.forename: X
authors.initials: X
authors.lastname: Guardino
authors.forename: C
authors.initials: C
authors.lastname: Serra
authors.forename: J
authors.initials: J
authors.lastname: Obiols
authors.forename: M G
authors.initials: MG
authors.lastname: Rosell
authors.forename: M J
authors.initials: MJ
authors.lastname: Berenguer
authors.forename: F
authors.initials: F
authors.lastname: López
authors.forename: J
authors.initials: J
authors.lastname: Brosa
mesh_terms: D000383:Agriculture; D002849:Chromatography, Gas / Q000379:methods*; D003634:DDT / Q000097:blood*; D003633:Dichlorodiphenyl Dichloroethylene / Q000097:blood; D003632:Dichlorodiphenyldichloroethane / Q000097:blood; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007306:Insecticides / Q000097:blood*; D016273:Occupational Exposure
chemical_list: D007306:Insecticides; D003633:Dichlorodiphenyl Dichloroethylene; D003634:DDT; D003632:Dichlorodiphenyldichloroethane