Immunohistochemical expression of glutathione S-transferase pi (GST-pi) and chemotherapy response in malignant ovarian tumors.
OBJECTIVE
To clarify the relationship between the expression of GST-pi and the therapeutic effects of high-dose combination chemotherapy with support by autologous bone-marrow transplantation (ABMT).


METHODS
Expression of GST-pi in tissue specimen were analyzed by indirect immunoperoxidase methods using anti-GST-pi polyclonal antibody.


RESULTS
(1) Eighteen of 36 cases (50%) tested prior to administration of any chemotherapeutic agents were found to be positive in terms of immunohistochemical expression of GST-pi in the cancer cells. (2) The rate of GST-pi expression was elevated following administration of cisplatin, adriamycin, and cyclophosphamide, which suggests that administration of such anti-cancer agents is capable of inducing expression of GST-pi in cancer cells. (3) GST-pi negative patients exhibit better survival rates than do GST-pi positive patients (Kaplan Meier method: p < 0.001).


CONCLUSIONS
Analysis of GST-pi obtained at primary surgery can serve as a valid marker in anticipating chemotherapeutic effects and long-term prognoses.
Hirazono
K
K
Department of Obstetrics and Gynecology, Tokai University School of Medicine, Kanagawa, Japan.
Shinozuka
T
T
Kuroshima
Y
Y
Itoh
H
H
Kawai
K
K
D000971:Antineoplastic Combined Chemotherapy Protocols / Q000627:therapeutic use; D005260:Female; D005982:Glutathione Transferase / Q000378:metabolism*; D006801:Humans; D007124:Immunoenzyme Techniques; D010051:Ovarian Neoplasms / Q000188:drug therapy* / Q000201:enzymology* / Q000401:mortality / Q000473:pathology; D011379:Prognosis; D016019:Survival Analysis; D016896:Treatment Outcome
D005982:Glutathione Transferase