Triton X-100 alters the resistance level of methicillin-resistant Staphylococcus aureus to oxacillin.
We used population analysis to examine the effects of Triton X-100 on the level of resistance to oxacillin of 18 methicillin-resistant Staphylococcus aureus. In the presence of 0.02% Triton-X-100, 17 formerly methicillin-resistant strains exhibited enhanced sensitivity to oxacillin. One homogenous isolate, KSAF1 was barely affected by the Triton X-100. Sensitivities of lysostaphin, 51 kDa N-acetylglucosaminidase and 62 kDa N-acetylmuramoyl-L- alanine amidase to heat-inactivated cells were not affected when the bacteria were grown in 0.02% Triton X-100. Our data, together with those of a previous study, suggested that Triton X-100 alters the resistance level of methicillin-resistant S. aureus influencing a factor (s) other than PBPs, bacteriolytic enzymes, or femAB products.
Komatsuzawa
H
H
Department of Microbiology, Hiroshima University School of Dentistry, Japan.
Sugai
M
M
Shirai
C
C
Suzuki
J
J
Hiramatsu
K
K
Suginaka
H
H
D000118:Acetylglucosaminidase / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D006358:Hot Temperature; D008248:Lysostaphin / Q000494:pharmacology; D016106:Methicillin Resistance; D009238:N-Acetylmuramoyl-L-alanine Amidase / Q000494:pharmacology; D017830:Octoxynol / Q000494:pharmacology*; D010068:Oxacillin / Q000494:pharmacology*; D010403:Penicillin Resistance; D010406:Penicillins / Q000494:pharmacology*; D010455:Peptides; D013211:Staphylococcus aureus / Q000187:drug effects*
D000900:Anti-Bacterial Agents; D010406:Penicillins; D010455:Peptides; D017830:Octoxynol; D000118:Acetylglucosaminidase; D008248:Lysostaphin; D009238:N-Acetylmuramoyl-L-alanine Amidase; D010068:Oxacillin