In vivo study of the immunomodulating activity of OM-85 using the plaque-forming cells technique (PFC) in mice.
The activity of increasing oral doses of the bacterial extract OM-85 was determined by using the plaque-forming cells assay in mice. The results showed that the oral administration of OM-85 significantly increased the number of splenic antibody-secreting cells against sheep red blood cells (antigen) compared with controls. A maximal response was reached between 400 and 800 mg/kg body weight, and was reproducible with the different batches of OM-85 used. This study indicates that the oral administration of the immunomodulator allows the activation of splenic B-lymphocytes, probably through the gut-associated lymphoid tissue.
Mimouni
J
J
Université Claude Bernard-Lyon I, Laboratoire de Physiologie 404, Villeurbanne, France.
Ramsteiner
A
A
Fontanges
R
R
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects*; D001419:Bacteria; D002457:Cell Extracts; D006462:Hemolytic Plaque Technique; D008213:Lymphocyte Activation / Q000187:drug effects*; D051379:Mice; D008807:Mice, Inbred BALB C; D013154:Spleen / Q000166:cytology / Q000187:drug effects; D000095983:Bacterial Lysates
D000276:Adjuvants, Immunologic; D002457:Cell Extracts; C030259:Broncho-Vaxom; D000095983:Bacterial Lysates