HPLC purification of synthetic oligodeoxyribonucleotides containing base- and backbone-modified sequences.
Applications for a new polymer resin, PolyFlo, are described for both the small-scale and large-scale purification of synthetic oligodeoxyribonucleotides varying in length from 18-41 bases. The unique properties of this innovative resin provide > 95% purified full-length oligodeoxyribonucleotides with greater than 90% yield starting from either crude trityl-on or trityl-off unmodified as well as base (biotin)- or backbone (e.g., phosphorothioate)-modified products. Full biological activity of recovered nucleic acid is retained, and the resin is capable of removing contaminating endotoxins during purification. The resin performance is predictable and reliable. The resin can be regenerated easily and is particularly economic when employed directly in ammonia or with the trityl-off option. PolyFlo meets the requirements of current Good Manufacturing Practices.
Green
A P
AP
Puresyn, Inc., Malvern, PA, USA.
Burzynski
J
J
Helveston
N M
NM
Prior
G M
GM
Wunner
W H
WH
Thompson
J A
JA
D064753:Ammonium Hydroxide; D001483:Base Sequence; D001710:Biotin; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D006878:Hydroxides; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000737:chemistry* / Q000302:isolation & purification*; D011108:Polymers; D015203:Reproducibility of Results; D012116:Resins, Plant
D006878:Hydroxides; D009838:Oligodeoxyribonucleotides; D011108:Polymers; D012116:Resins, Plant; D064753:Ammonium Hydroxide; D001710:Biotin