Gene mapping in the common shrew (Sorex araneus; Insectivora) by shrew-rodent cell hybrids: chromosome localization of the loci for HPRT, TK, LDHA, MDH1, G6PD, PGD, and ADA.
We selected the common shrew (Sorex araneus) to generate the first insectivore gene map. Shrew-Chinese hamster and shrew- mouse somatic cell hybrid cells were constructed. When the 119 shrew-rodent clones were characterized, only shrew chromosomes were found to have segregated. A panel of hybrid clones was selected for gene assignment. The genes for hypoxanthine phosphoribosyl transferase (HPRT), glucose-6- phosphate dehydrogenase (G6PD), and malate dehydrogenase 1 (MDH1) were assigned to shrew Chromosome (Chr) de [which is the product of a tandem fusion between the 'original' mammalian X Chromosome (Chr) and an autosome], the gene for adenosine deaminase (ADA) and 6-phosphogluconate dehydrogenase se (PGD) to Chromosome jl, the gene for thymidine kinase (TK) to Chromosome hn, and the gene for lactate dehydrogenase (LDHA) to chromosome ik. Further studies in progress.
Pack
S D
SD
Laboratory of Developmental Genetics. Academy of Sciences of Russia, Novosibirsk, Russia.
Kolonin
M G
MG
Borodin
P M
PM
Searle
J B
JB
Serov
O L
OL
D000243:Adenosine Deaminase / Q000235:genetics; D000818:Animals; D002478:Cells, Cultured; D002874:Chromosome Mapping; D002999:Clone Cells; D006224:Cricetinae; D003412:Cricetulus; D004798:Enzymes / Q000235:genetics*; D005954:Glucosephosphate Dehydrogenase / Q000235:genetics; D006822:Hybrid Cells; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics; D007770:L-Lactate Dehydrogenase / Q000235:genetics; D008291:Malate Dehydrogenase / Q000235:genetics; D051379:Mice; D010734:Phosphogluconate Dehydrogenase / Q000235:genetics; D012788:Shrews / Q000235:genetics*; D013937:Thymidine Kinase / Q000235:genetics
D004798:Enzymes; D007770:L-Lactate Dehydrogenase; D008291:Malate Dehydrogenase; D010734:Phosphogluconate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D007041:Hypoxanthine Phosphoribosyltransferase; D013937:Thymidine Kinase; D000243:Adenosine Deaminase