Immunoglobulins anti-endonuclease 32 kDa from Cucurbita pepo var patissonina affect process of DNA synthesis.
Immunoglobulins anti-endonuclease 32 kDa inhibit DNA synthesis. We observed that low concentrations of IgGs (about 50 micrograms IgG per 1 x 10(6) cell nuclei) temporary inhibit DNA synthesis. This inhibition concerns only the synthesis of DNA bound to the nuclear matrix (associated with isolated nuclear matrix). Preincubation of cell nuclei of White bush with IgG generates longer DNA fragments than in controls. Involvement of the 32 kDa endonuclease or an endonuclease-65 kDa protein complex from the nuclear matrix in replication or structural organisation of replication is considered.
Rzepecki
R
R
Institute of Biochemistry, Wrocław University, Poland.
Szopa
J
J
D018744:DNA, Plant / Q000096:biosynthesis*; D004268:DNA-Binding Proteins / Q000378:metabolism; D004720:Endonucleases / Q000276:immunology*; D005069:Evaluation Studies as Topic; D007074:Immunoglobulin G / Q000032:analysis*; D015530:Nuclear Matrix / Q000378:metabolism; D010945:Plants, Edible / Q000201:enzymology / Q000276:immunology*
D018744:DNA, Plant; D004268:DNA-Binding Proteins; D007074:Immunoglobulin G; D004720:Endonucleases