title: 13-cis-Retinoic acid affect sheath-shaft interaction of equine hair follicles in vitro.
abstract: A major challenge to the study of hair follicle growth is an appropriate assay system. Because equine mane follicles are large and noncurved, enabling easy dissection; are readily accessible from a single defined source; and possess a long anagen growth phase, we initiated a study of them in culture. As in our previous studies of human and sheep follicles (Dev Biol 165:469, 1994), we found in this system that transection level dictates the pattern of follicle growth in vitro: follicles transected below the sebaceous gland show a type 1 growth pattern (the shaft grows out with an adherent sheath), while nontransected follicles show a type 2 growth pattern (a naked shaft grows out lacking a sheath). In the present study, we searched for compounds that might influence type 1 or type 2 patterns of growth. We found that 13-cis retinoic acid induced, in a concentration-dependent fashion, a type 1-like pattern of growth under conditions for which a type 2 pattern was expected. All-trans-retinoic acid, SR11237 (a synthetic retinoid X receptor-specific ligand), and meta-carboxy-TTNPB (and inactive synthetic retinoid) did not have these properties. We hypothesize that sheath growth¿processing is mediated by the follicle at the level of the sebaceous gland, or by the sebaceous gland itself, and that persistence of the follicle sheath about the outgrowing shaft in vitro (i) in the physical absence of the sebaceous portion of the follicle, or (ii) in the presence of 13-cis-retinoic acid, is due to the reduced expression of a factor that regulates important shaft sheath interactions.
authors.affiliation: Skin Biology Research Center of Johnson & Johnson, Skillman, NJ 08558-9418, USA.
authors.forename: D
authors.initials: D
authors.lastname: Williams
authors.forename: P
authors.initials: P
authors.lastname: Siock
authors.forename: K
authors.initials: K
authors.lastname: Stenn
mesh_terms: D000818:Animals; D001345:Autoradiography; D003470:Culture Media; D046508:Culture Techniques; D018859:Hair Follicle / Q000033:anatomy & histology / Q000187:drug effects* / Q000254:growth & development; D006736:Horses; D015474:Isotretinoin / Q000494:pharmacology*
chemical_list: D003470:Culture Media; D015474:Isotretinoin