Novel specific chemtactic receptor for S100L protein on guinea pig eosinophils.
We isolated a new chemotactic protein from bovine lung, and its partial protein sequence analysis indicated that this protein was identical with S100L, one member of the Ca2+-binding S100 protein family. The chemotactic activity of S100L on guinea pig eosinophils is observed at 0.1nM protein concentration, and the effects appear mediated by a novel specific surface receptor. We characterized the receptor for S100L on guinea pig eosinophils. Scatchard analyses of the data that [125I]S100L bound to S100L receptor indicate the presence of two receptor populations on eosinophils with a Kd value of 3.3 x 10(-11)M and 1.1 x 10(-9)M. Thus, S100L protein has the most potent chemotactic activity on guinea pig eosinophils of all the chemotactic proteins.
Komada
T
T
Department of Molecular and Cellular Pharmacology, Mie University School of Medicine, Japan.
Araki
R
R
Nakatani
K
K
Yada
I
I
Naka
M
M
Tanaka
T
T
D000818:Animals; D001921:Brain / Q000378:metabolism; D002417:Cattle; D002630:Chemotactic Factors / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology*; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D004804:Eosinophils / Q000187:drug effects / Q000502:physiology*; D006168:Guinea Pigs; D007700:Kinetics; D008168:Lung / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism; D044042:Receptors, Formyl Peptide; D011971:Receptors, Immunologic / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D018000:Receptors, Peptide / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D009418:S100 Proteins / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology*; D013379:Substrate Specificity; D013552:Swine; D010566:Virulence Factors, Bordetella / Q000494:pharmacology
D002630:Chemotactic Factors; D044042:Receptors, Formyl Peptide; D011971:Receptors, Immunologic; D018000:Receptors, Peptide; D009418:S100 Proteins; C098997:S100A2 protein, human; D010566:Virulence Factors, Bordetella