title: Transcriptional regulation of repressor synthesis in mycobacteriophage L5.
abstract: Mycobacteriophage L5 is a temperate phage of the mycobacteria that forms stable lysogens in Mycobacterium smegmatis. Lysogeny is maintained by the putative repressor, the gene 71 product, which also mediates immunity to superinfection. We show here that there are three promoters located upstream of gene 71 which are active in an L5 lysogen but which do not require any phage-encoded proteins. In early lytic growth, gene 71 is also transcribed from a promoter, Pleft, located at the right end of the genome and which appears to be a target of gp71 regulation. A model is given for the regulation of L5 life cycles.
authors.affiliation: Department of Biological Sciences, University of Pittsburgh, Pennsylvania 15260, USA.
authors.forename: C E
authors.initials: CE
authors.lastname: Nesbit
authors.forename: M E
authors.initials: ME
authors.lastname: Levin
authors.forename: M K
authors.initials: MK
authors.lastname: Donnelly-Wu
authors.forename: G F
authors.initials: GF
authors.lastname: Hatfull
mesh_terms: D000595:Amino Acid Sequence; D001433:Bacteriolysis; D001483:Base Sequence; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D015967:Gene Expression Regulation, Viral; D008242:Lysogeny; D008969:Molecular Sequence Data; D016296:Mutagenesis; D009160:Mycobacteriophages / Q000235:genetics* / Q000502:physiology; D009161:Mycobacterium / Q000378:metabolism / Q000821:virology*; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000096:biosynthesis; D012367:RNA, Viral / Q000096:biosynthesis; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D012097:Repressor Proteins / Q000096:biosynthesis / Q000235:genetics*; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D004268:DNA-Binding Proteins; D012333:RNA, Messenger; D012367:RNA, Viral; D011993:Recombinant Fusion Proteins; D012097:Repressor Proteins; D014764:Viral Proteins; C080483:gp71 protein, Mycobacteriophage L5