Conversion studies with substrate analogues of toluene in a sulfate-reducing bacterium, strain Tol2.
Anaerobic toluene oxidation by the sulfate-reducing bacterium, strain Tol2 (proposed name Desulfobacula toluolica) was specifically inhibited by benzyl alcohol when added at concentrations around 500 microM. Benzyl alcohol added at lower, non-inhibitory concentrations (around 5 microM) was not oxidized by active cells pregrown on toluene, indicating that the alcohol is not a free intermediate of toluene metabolism in the sulfate reducer. Conversion of p-xylene in toluene-metabolizing cells to p-methylbenzoate as dead-end product suggests that the sulfate reducer, like denitrifiers, initiates toluene oxidation at the methyl group.
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Max-Planck-Institut für Marine Mikrobiologie, Fahrenheitstrasse 1, D-28359 Bremen, Germany.
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D001421:Bacteria, Anaerobic / Q000378:metabolism*; D019905:Benzyl Alcohol; D001592:Benzyl Alcohols / Q000378:metabolism; D001673:Biodegradation, Environmental; D010084:Oxidation-Reduction; D013431:Sulfates / Q000378:metabolism*; D014050:Toluene / Q000378:metabolism*
D001592:Benzyl Alcohols; D013431:Sulfates; D014050:Toluene; D019905:Benzyl Alcohol