title: Mapping of murine YACs containing the genes Cea2 and Cea4 after B1-PCR amplification and FISH-analysis.
abstract: PCR with primers specific for the murine B1 consensus sequence allows amplification of DNA from murine sources. We have used B1-PCR for amplifying yeast artificial chromosome (YAC) DNA which can be used to localize single YACs by fluorescence in situ hybridization. The genes for the pregnancy-specific glycoproteins Cea2 and Cea4, both belonging to the large carcinoembryonic antigen gene family, were localized by chromosomal in situ suppression hybridization of three YAC clones to murine chromosome 7A2-A3. This was facilitated by the use of the mouse lymphoma cell line WMP/WMP which contains nine pairs of Robertsonian fusion chromosomes.
authors.affiliation: Abteilung Medizinische Genetik, Universität Ulm, Germany.
authors.forename: G
authors.initials: G
authors.lastname: Rettenberger
authors.forename: W
authors.initials: W
authors.lastname: Zimmermann
authors.forename: C
authors.initials: C
authors.lastname: Klett
authors.forename: U
authors.initials: U
authors.lastname: Zechner
authors.forename: H
authors.initials: H
authors.lastname: Hameister
mesh_terms: D000818:Animals; D015139:Blotting, Southern; D002874:Chromosome Mapping; D002875:Chromosomes / Q000648:ultrastructure; D018244:Chromosomes, Artificial, Yeast / Q000235:genetics*; D016384:Consensus Sequence; D004274:DNA, Recombinant / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D006023:Glycoproteins / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D016133:Polymerase Chain Reaction; D011257:Pregnancy Proteins / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid
chemical_list: D004274:DNA, Recombinant; D006023:Glycoproteins; D011257:Pregnancy Proteins