title: Transcript mapping and processing of mitochondrial RNA in the chlorophyte alga Prototheca wickerhamii.
abstract: The detailed transcript map of the circular 55328 bp mitochondrial (mt) genome from the colourless chlorophycean alga Prototheca wickerhamii has been determined. On each half of this genome the genes are encoded only on one DNA strand, forming transcriptional units comprising variable numbers of genes. With the exception of four genes coding for ribosomal proteins, transcripts of the three rRNA genes and all protein-coding genes have been detected by both northern analysis and primer extension experiments. Polycistronic transcripts of protein coding and tRNA genes were verified by northern analyses, primer extension and RNAse mapping experiments. The 5' and 3' ends of different RNA species are often located in close proximity to putative stem-loop structures and some 5' termini of mRNAs coincide with the 3' end of tRNAs located immediately upstream. Transcript mapping in a putative promoter region revealed two different possible transcription initiation sites; no significant sequence homology to putative mt promoters from higher plants could be found. In addition, two out of three group I introns residing in the cox1 gene were found to be self-splicing in vitro under reaction conditions developed for related mt introns from a filamentous fungus. Mitochondrial gene expression of P. wickerhamii and of filamentous fungi has several features in common, such as intron splicing and the processing of longer polycistronic transcripts. The similarities in RNA maturation between higher-plant and P. wickerhamii mitochondria are less pronounced, since plants rarely use tRNAs as processing signals for their relatively short mitochondrial co-transcripts.
authors.affiliation: Lehrstuhl für Allgemeine Botanik, Ruhr-Universität Bochum, Germany.
authors.forename: G
authors.initials: G
authors.lastname: Wolff
authors.forename: U
authors.initials: U
authors.lastname: Kück
mesh_terms: D001483:Base Sequence; D015152:Blotting, Northern; D002384:Catalysis; D002874:Chromosome Mapping; D018744:DNA, Plant; D017343:Genes, Plant; D007438:Introns; D008928:Mitochondria / Q000235:genetics*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D016366:Open Reading Frames; D011401:Promoter Regions, Genetic; D011525:Prototheca / Q000235:genetics*; D012313:RNA / Q000737:chemistry / Q000235:genetics; D012323:RNA Processing, Post-Transcriptional; D012326:RNA Splicing; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics*; D000077278:RNA, Mitochondrial; D014158:Transcription, Genetic
chemical_list: D018744:DNA, Plant; D012333:RNA, Messenger; D000077278:RNA, Mitochondrial; D012313:RNA