UV-induced photoproducts in human skin explants analysed by TLC and HPLC-radioactivity detection.
The objective of this study was to apply the 32P-postlabelling method for the detection of UV-induced dipyrimidine photoadducts in human skin explants. [32P]TLC and [32P]HPLC analysis were used. These techniques allow detection of different combinations of cyclobutane dimers and 6-4 photoproducts in human skin at low doses of irradiation. Detection limit of the present method for some dinucleotide adducts is < 1 adduct per 10(7) normal nucleotides. Reaction of photochemical reversion of the cyclobutane dimers was performed to confirm the nature of the products detected in human skin explants.
Bykov
V J
VJ
Center for Nutrition and Toxicology, Karolinska Institute, Huddinge, Sweden.
Hemminki
K
K
D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D018736:DNA Adducts; D004307:Dose-Response Relationship, Radiation; D006801:Humans; D066298:In Vitro Techniques; D010761:Phosphorus Radioisotopes; D011743:Pyrimidines; D011874:Radiometry; D012867:Skin / Q000378:metabolism / Q000528:radiation effects*; D014466:Ultraviolet Rays
D018736:DNA Adducts; D010761:Phosphorus Radioisotopes; D011743:Pyrimidines