The expression of a specific 2-deoxyglucose-6P phosphatase prevents catabolite repression mediated by 2-deoxyglucose in yeast.
2-deoxyglucose (2-DOG), a non-metabolize analogue of glucose, is taken up by yeast using the same transporter(s) as glucose and is phosphorylated by hexokinases producing 2-deoxyglucose-6-P. We found that in DOGR yeasts, 2-DOG was not able to trigger glucose repression, even at concentrations of 0.5%. This result suggests that the specific 2-DOG-6P phosphatase, the enzyme responsible for the DOGR phenotype, may be involved in inhibiting the process of catabolite repression mediated by 2-DOG.
Randez-Gil
F
F
Departamento Biotecnología, Instituto Agroquínica y Tecnología de los Alimentos, (C.S.I.C.), Valencia, Spain.
Prieto
J A
JA
Sanz
P
P
D003847:Deoxyglucose / Q000235:genetics / Q000378:metabolism*; D005800:Genes, Fungal; D007700:Kinetics; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism*; D012097:Repressor Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins; D014170:Transformation, Genetic
D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; D003847:Deoxyglucose; C093600:DOG2 protein, S cerevisiae; D010744:Phosphoric Monoester Hydrolases