title: Expression, purification, and assay of Sec12p: a Sar1p-specific GDP dissociation stimulator.
authors.affiliation: Department of Biochemistry, Dartmouth Medical School, Hanover, New Hampshire 03755, USA.
authors.forename: C
authors.initials: C
authors.lastname: Barlowe
authors.forename: R
authors.initials: R
authors.lastname: Schekman
mesh_terms: D002462:Cell Membrane / Q000378:metabolism; D002850:Chromatography, Gel / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000379:methods; D005656:Fungal Proteins / Q000096:biosynthesis* / Q000302:isolation & purification* / Q000378:metabolism; D019204:GTP-Binding Proteins / Q000378:metabolism*; D005800:Genes, Fungal; D020662:Guanine Nucleotide Exchange Factors; D006153:Guanosine Diphosphate / Q000378:metabolism; D007202:Indicators and Reagents; D007700:Kinetics; D008562:Membrane Glycoproteins / Q000096:biosynthesis* / Q000302:isolation & purification* / Q000378:metabolism; D020559:Monomeric GTP-Binding Proteins; D011865:Radioisotope Dilution Technique; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins; D014316:Tritium; D033921:Vesicular Transport Proteins
chemical_list: D005656:Fungal Proteins; D020662:Guanine Nucleotide Exchange Factors; D007202:Indicators and Reagents; D008562:Membrane Glycoproteins; C071239:SEC12 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D033921:Vesicular Transport Proteins; D014316:Tritium; D006153:Guanosine Diphosphate; D019204:GTP-Binding Proteins; D020559:Monomeric GTP-Binding Proteins; C077395:SAR1 protein, S cerevisiae