Activation of the inactive X chromosome induced by cell fusion between a murine EC and female somatic cell accompanies reproducible changes in the methylation pattern of the Xist gene.
Mouse embryonal carcinoma (EC) cell lines are divided into two classes with or without the capability of reactivating the inactive X chromosome from a fusion partner of female lymphocyte. The 5' region of Xist was partially methylated in reactivating-competent EC cells but was fully methylated in reactivating-incompetent EC cells having a single X chromosome. Partial or heterogeneous methylation implies methylation of each CpG site in about half of the cell independently of methylation status of neighboring CpG sites. Fusion of the reactivating-competent EC cells with female lymphocytes induced not only de novo methylation in the 5' region of Xist allele on the hitherto inactivated X chromosome, but also demethylation of the same region of Xist on the other X chromosome from the female somatic cell. In contrast, no such changes occurred in hybrid cells involving reactivating-incompetent EC cells. Thus, partial methylation of the 5' region of Xist most probably maintained by low maintenance and high de novo methylation efficiency is correlated with reactivation potential of the EC cell. It is possible that this unique methylation pattern is implicated in random X inactivation in EC-hybrid cells in vitro and in epiblast cells in vivo.
Mise
N
N
Division of Bioscience, Graduate School of Environmental Earth Science, Hokkaido University, Sapporo, Japan.
Sado
T
T
Tada
M
M
Takada
S
S
Takagi
N
N
D000818:Animals; D001483:Base Sequence; D002459:Cell Fusion; D002678:Chimera / Q000235:genetics*; D018899:CpG Islands / Q000502:physiology; D004247:DNA / Q000378:metabolism*; D004303:Dosage Compensation, Genetic; D054278:Embryonal Carcinoma Stem Cells; D005260:Female; D005786:Gene Expression Regulation; D008214:Lymphocytes / Q000378:metabolism; D008297:Male; D008745:Methylation; D051379:Mice; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D014411:Neoplastic Stem Cells / Q000378:metabolism; D062085:RNA, Long Noncoding; D022661:RNA, Untranslated; D014157:Transcription Factors / Q000235:genetics*; D014960:X Chromosome / Q000378:metabolism*
D062085:RNA, Long Noncoding; D022661:RNA, Untranslated; D014157:Transcription Factors; C077885:XIST non-coding RNA; D004247:DNA