title: Phylogenetic evidence that the gram-negative nonsporulating bacterium Tissierella (Bacteroides) praeacuta is a member of the Clostridium subphylum of the gram-positive bacteria and description of Tissierella creatinini sp. nov.
abstract: The 16S rRNA gene sequence of the type strain of Tissierella praeacuta (formerly Bacteroides praeacutus) was determined by PCR direct sequencing. A comparative sequence analysis showed that T. praeacuta is a member of the Clostridium subphylum of the gram-positive bacteria and has a close phylogenetic affinity with the species that form Clostridium cluster XII (M. D. Collins, P. A. Lawson, A. Willems, J. J. Cordoba, J. Fernandez-Garayzabal, P. Garcia, J. Cai, H. Hippe, and J. A. E. Farrow, Int. J. Syst. Bacteriol. 44:812-826, 1994). Although T. praeacuta is gram negative and does not produce endospores, 16S rRNA sequence data showed that it is closely related genealogically (level of sequence similarity, 99.9%) to Clostridium hastiforme. On the basis of our results and the results of previous studies, a second species of Tissierella, Tissierella creatinini sp. nov., is described.
authors.affiliation: Institute of Food Research, Reading Laboratory, United Kingdom.
authors.forename: J A
authors.initials: JA
authors.lastname: Farrow
authors.forename: P A
authors.initials: PA
authors.lastname: Lawson
authors.forename: H
authors.initials: H
authors.lastname: Hippe
authors.forename: U
authors.initials: U
authors.lastname: Gauglitz
authors.forename: M D
authors.initials: MD
authors.lastname: Collins
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D016667:Bacterial Capsules / Q000096:biosynthesis; D002473:Cell Wall / Q000737:chemistry; D003013:Clostridium / Q000145:classification* / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry; D006089:Gram-Negative Anaerobic Bacteria / Q000145:classification* / Q000235:genetics / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007093:Imidazoles / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000737:chemistry*; D012689:Sequence Homology, Nucleic Acid; D013696:Temperature
chemical_list: D000596:Amino Acids; D004275:DNA, Ribosomal; D007093:Imidazoles; D012336:RNA, Ribosomal, 16S