Isolation and mapping of human homologues of an imprinted mouse gene U2af1-rs1.
We have isolated human homologues of the imprinted mouse gene, U2af1-rs1. Two different types of cDNAs and three distinct genomic DNAs belonging to different groups were isolated. We have identified chromosomal genes corresponding to each cDNA by restriction mapping and sequencing. Using both a panel of rodent/human somatic cell hybrids and fluorescence in situ hybridization, group 1 and group 2 genes were mapped to chromosome 5q22 and chromosome Xp22.1, respectively. We designated group 1 and group 2 genes as human U2AF1-RS1 and U2AF1-RS2, respectively, because these genes corresponded to mouse U2af1-rs1 (chromosome 11) and U2af1-rs2 (chromosome X), which we also isolated and mapped. Amino acid sequences of human U2AF1-RS1 and U2AF-RS2 showed significant homology to U2AF small subunit. The group 3 gene, designated as U2AF1-RS3, of which the cDNA has not yet been isolated, was mapped to chromosome 19p13.2.
Kitagawa
K
K
Department of Bioscience, National Cardiovascular Center Research Institute, Osaka, Japan.
Wang
X
X
Hatada
I
I
Yamaoka
T
T
Nojima
H
H
Inazawa
J
J
Abe
T
T
Mitsuya
K
K
Oshimura
M
M
Murata
A
A
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D002895:Chromosomes, Human, Pair 5; D003001:Cloning, Molecular; D018076:DNA, Complementary; D018392:Genomic Imprinting; D006801:Humans; D006822:Hybrid Cells; D051379:Mice; D008969:Molecular Sequence Data; D005810:Multigene Family; D009419:Nerve Tissue Proteins; D009687:Nuclear Proteins / Q000235:genetics*; D011506:Proteins; D012261:Ribonucleoproteins; D017386:Sequence Homology, Amino Acid; D000072668:Splicing Factor U2AF
D018076:DNA, Complementary; D009419:Nerve Tissue Proteins; D009687:Nuclear Proteins; D011506:Proteins; D012261:Ribonucleoproteins; D000072668:Splicing Factor U2AF; C486290:U2AF1L4 protein, human; C484334:ZRSR2 protein, human; C098232:ZRSR2P1 protein, human; C000607495:Zrsr1 protein, mouse; C096074:Zrsr2 protein, mouse