Structural and functional analysis of the 5'-flanking region of the rat beta 2-adrenergic receptor gene.
We have analyzed 3.4 kb of DNA from the 5'-flanking region of the rat beta 2-adrenergic receptor gene and assessed its promoter activity in A549 cells, a human lung adenocarcinoma cell line. A single transcription start site was identified approx. 223 bp upstream of the ATG start codon. A549 cells were transfected with luciferase reporter plasmids containing segments of the rat beta 2-adrenergic receptor 5'-flanking region. Our results suggest that both positive and negative cis-acting regulatory sequences are present in the 5'-flanking region of the rat beta 2-adrenergic receptor gene.
McGraw
D W
DW
Department of Medicine, University of Arkansas for Medical Sciences, Little Rock, 72205, USA.
Jacobi
S E
SE
Hiller
F C
FC
Cornett
L E
LE
D000230:Adenocarcinoma; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002460:Cell Line; D003062:Codon; D004247:DNA / Q000737:chemistry / Q000302:isolation & purification; D017931:DNA Primers; D004268:DNA-Binding Proteins / Q000378:metabolism; D006801:Humans; D008156:Luciferases / Q000096:biosynthesis; D008175:Lung Neoplasms; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011401:Promoter Regions, Genetic; D051381:Rats; D018343:Receptors, Adrenergic, beta-2 / Q000235:genetics*; D011994:Recombinant Proteins / Q000096:biosynthesis; D015183:Restriction Mapping; D016385:TATA Box; D014158:Transcription, Genetic; D014162:Transfection; D014407:Tumor Cells, Cultured
D003062:Codon; D017931:DNA Primers; D004268:DNA-Binding Proteins; D018343:Receptors, Adrenergic, beta-2; D011994:Recombinant Proteins; D004247:DNA; D008156:Luciferases