Gas chromatographic procedure for simultaneous determination of selegiline metabolites, amphetamine, methamphetamine and demethyl-deprenyl in pig plasma.
A sensitive assay for the simultaneous quantitative determination of amphetamine, methamphetamine and demethyl-deprenyl in pig plasma is described. NP-Gas chromatography is used to determine the extracted plasma concentrations of the three target compounds as their N-penta-fluoro-benzoyl derivatives. Quantitation is performed using 1-phenyl-2-pentylamine as internal standard. The derivatives are separated on a phenyl-methylsilicone capillary column. Quantitation limit for each target compound was 1.2 ng ml-1. Levels of amphetamine, methamphetamine and demethyl-deprenyl have been determined in plasma of pigs treated with Selegiline in different formulations and doses.
Szebeni
G
G
Chinoin Pharmaceutical Works, Pharmacokinetics and Metabolism Laboratory, Budapest, Hungary.
Lengyel
J
J
Székács
G
G
Magyar
K
K
Gaál
J
J
Szatmári
I
I
D000661:Amphetamine / Q000097:blood / Q000493:pharmacokinetics; D000662:Amphetamines / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D000818:Animals; D002214:Capsules; D000697:Central Nervous System Stimulants / Q000097:blood / Q000493:pharmacokinetics; D002849:Chromatography, Gas; D005260:Female; D007202:Indicators and Reagents; D008694:Methamphetamine / Q000097:blood / Q000493:pharmacokinetics; D008996:Monoamine Oxidase Inhibitors / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D012642:Selegiline / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D013552:Swine; D013607:Tablets
D000662:Amphetamines; D002214:Capsules; D000697:Central Nervous System Stimulants; D007202:Indicators and Reagents; D008996:Monoamine Oxidase Inhibitors; D013607:Tablets; D012642:Selegiline; D008694:Methamphetamine; C017861:desmethylselegiline; D000661:Amphetamine