Selective binding and transcytosis of Ulex europaeus 1 lectin by mouse Peyer's patch M-cells in vivo.
The in vivo interaction of the lectin Ulex europaeus agglutinin 1 with mouse Peyer's patch follicle-associated epithelial cells was studied in the mouse Peyer's patch gut loop model by immunofluorescence and electron microscopy. The lectin targets to mouse Peyer's patch M-cells and is rapidly endocytosed and transcytosed. These processes are accompanied by morphological changes in the M-cell microvilli and by redistribution of polymerised actin. The demonstration of selective binding and uptake of a lectin by intestinal M-cells in vivo suggests that M-cell-specific surface glycoconjugates might act as receptors for the selective adhesion/uptake of microorganisms.
Clark
M A
MA
Gastrointestinal Drug Delivery Research Centre and Department of Physiological Sciences, University of Newcastle upon Tyne, Medical School, Newcastle upon Tyne NE2 4HH, UK.
Jepson
M A
MA
Simmons
N L
NL
Hirst
B H
BH
D000199:Actins / Q000378:metabolism; D000818:Animals; D001692:Biological Transport; D004705:Endocytosis; D005260:Female; D037102:Lectins / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008871:Microvilli / Q000378:metabolism / Q000648:ultrastructure; D010581:Peyer's Patches / Q000166:cytology / Q000378:metabolism*; D037121:Plant Lectins; D011485:Protein Binding
D000199:Actins; D037102:Lectins; D037121:Plant Lectins; C036850:Ulex europaeus lectins