title: Phenotypic analysis of neoplastic cells from calf, thymic, and intermediate forms of bovine leukosis.
abstract: Immunohistochemistry and flow cytometric analysis were used with monoclonal antibodies to examine the phenotype of neoplastic cells from cattle with sporadic bovine leukosis (three cases of calf form, two cases of thymic form, and three cases of intermediate form). Three cases of calf form and two cases of intermediate form were positive for B cell lineage in immunohistologic examination and in flow cytometric analysis for B-B2+, sIgM+, and major histocompatibility class II+. Two cases of thymic form and one case of intermediate form were CD2+, CD5+, CD6+, and CD8+ in immunohistologic examination and in flow cytometric analysis. The results show that neoplastic cells develop from B and T cell lineages in sporadic bovine leukosis.
authors.affiliation: Department of Veterinary Pathology, Faculty of Agriculture, Iwate University, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Asahina
authors.forename: K
authors.initials: K
authors.lastname: Kimura
authors.forename: K
authors.initials: K
authors.lastname: Murakami
authors.forename: T
authors.initials: T
authors.lastname: Ajito
authors.forename: D
authors.initials: D
authors.lastname: Wu
authors.forename: M
authors.initials: M
authors.lastname: Goryo
authors.forename: Y
authors.initials: Y
authors.lastname: Aida
authors.forename: W C
authors.initials: WC
authors.lastname: Davis
authors.forename: K
authors.initials: K
authors.lastname: Okada
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000032:analysis; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D001402:B-Lymphocytes / Q000276:immunology / Q000473:pathology*; D001483:Base Sequence; D018801:CD2 Antigens / Q000032:analysis; D018956:CD5 Antigens / Q000032:analysis; D016827:CD8 Antigens / Q000032:analysis; D002417:Cattle; D017931:DNA Primers / Q000737:chemistry; D016583:Enzootic Bovine Leukosis / Q000145:classification* / Q000473:pathology*; D005260:Female; D005434:Flow Cytometry / Q000662:veterinary; D007150:Immunohistochemistry; D007668:Kidney / Q000276:immunology / Q000473:pathology; D008099:Liver / Q000276:immunology / Q000473:pathology; D008198:Lymph Nodes / Q000276:immunology / Q000473:pathology; D008297:Male; D008969:Molecular Sequence Data; D010641:Phenotype; D010994:Pleura / Q000276:immunology / Q000473:pathology; D016133:Polymerase Chain Reaction / Q000662:veterinary; D013154:Spleen / Q000276:immunology / Q000473:pathology; D013601:T-Lymphocytes / Q000276:immunology / Q000473:pathology*; D013950:Thymus Gland / Q000276:immunology / Q000473:pathology
chemical_list: D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D018801:CD2 Antigens; D018956:CD5 Antigens; C062279:CD6 antigen; D016827:CD8 Antigens; D017931:DNA Primers