On the nature of the adaptive response induced by mitomycin C in Vibrio cholerae OGAWA 154 cells.
The induction of an adaptive response in Vibrio cholerae OGAWA 154 cells was obtained using an alkylating agent, mitomycin C, as both stimulating and challenging agent. Cross-adaptive response was observed in V. cholerae cells when pretreated with a sublethal dose of another alkylating agent, N-methyl-N'nitro-N-nitrosoguanidine, followed by challenging treatment with mitomycin C. The dose of mitomycin C for 50% survival (D50) became almost double for mitomycin C pretreated cells and 1.5 times for N-methyl-N-'nitro-N-nitrosoguanidine pretreated cells, compared to nonpretreated cells. It was also shown that pretreatment with a sublethal dose of oxidative DNA damaging agents, viz, hydrogen peroxide or nitrofurantoin, did not show any cross-adaptive response against subsequent challenge by mitomycin C.
Basak
J
J
Biophysics Laboratory, Saha Institute of Nuclear Physics, Calcutta, India.
D000064:Acclimatization; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D018906:Antineoplastic Agents, Alkylating / Q000494:pharmacology*; D004249:DNA Damage; D004305:Dose-Response Relationship, Drug; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007700:Kinetics; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology; D016685:Mitomycin / Q000494:pharmacology*; D009582:Nitrofurantoin / Q000494:pharmacology; D013997:Time Factors; D014734:Vibrio cholerae / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development
D000903:Antibiotics, Antineoplastic; D018906:Antineoplastic Agents, Alkylating; D008769:Methylnitronitrosoguanidine; D016685:Mitomycin; D009582:Nitrofurantoin; D006861:Hydrogen Peroxide