(3H)hemicholinium-3 binding sites in postmortem brains of human patients with Alzheimer's disease and multi-infarct dementia.
(3H)Hemicholinium-3 ((3H)HCh-3), a potent, selective, and competitive inhibitor of the high-affinity choline uptake process was used for the detection of high-affinity choline carriers in the hippocampus (gyrus parahippocampalis), neocortex (gyrus frontalis medius), and cerebellum (lobulus semilunaris inferior) in autopsy samples of people with Alzheimer's disease, multi-infarct dementia and from other psychiatric and nonpsychiatric patients. The effect of postmortem delay was eliminated by means of the cerebellum used as an individual standard. The density of (3H)HCh-3 binding sites was decreased in the hippocampus and neocortex from individuals with multi-infarct dementia and unchanged in the brain tissue from people with Alzheimer's disease in comparison with control patients. No changes in dissociation constants were found. In Alzheimer's disease, high-affinity choline transport appears to be reduced by a dysfunction of cholinergic neuronal membrane rather than by a significant decrease in the number of presynaptic cholinergic nerve terminals. Results provide evidence of a decrease in the number of nerve endings in people with multi-infarct dementia and suggest different vulnerability of particular brain areas to vascular disorders.
Kristofiková
Z
Z
Prague Psychiatric Centre, Czech Republic.
Fales
E
E
Majer
E
E
Klaschka
J
J
D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000378:metabolism*; D000704:Analysis of Variance; D001344:Autopsy; D001665:Binding Sites; D001667:Binding, Competitive; D016022:Case-Control Studies; D002794:Choline / Q000378:metabolism*; D015161:Dementia, Multi-Infarct / Q000378:metabolism*; D005260:Female; D006426:Hemicholinium 3 / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged; D014316:Tritium
D014316:Tritium; D006426:Hemicholinium 3; D002794:Choline