Cloning and expression of a 5HT7 receptor from Xenopus laevis.
A clone encoding a 5TH7 receptor, XM2, has been isolated from Xenopus laevis. The mRNA encoding XM2 is expressed in brain, liver, muscle, kidney, intestine and skin, but not in heart or lung. Co-expression of XM2 and the cystic fibrosis transmembrane conductance regulator (CFTR) in Xenopus oocytes demonstrated coupling of the receptor and the channel via the protein kinase A pathway. A rank order of potency was obtained for serotonergic agonists of 5-hydroxytryptamine (5HT) > or = 5-methoxytryptamine (5MeOxyT) > or = alpha-methyl-5-hydroxytryptamine (alpha Me5HT) > 8-OH-DPAT, and for serotonergic antagonists of ritanserin > NAN190 > mesulergine not equal to pindolol. Thus, XM2 encodes a 5HT receptor with sequence homology to mammalian 5HT7 receptors, a similar intracellular signal transduction and receptor pharmacology, but a broader distribution in peripheral tissues.
Nelson
C S
CS
Center for Research on Occupational and Environmental Toxicology L606, Oregon Health Sciences University, Portland 97201, USA.
Cone
R D
RD
Robbins
L S
LS
Allen
C N
CN
Adelman
J P
JP
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000235:genetics; D004247:DNA / Q000235:genetics; D005260:Female; D015870:Gene Expression; D006801:Humans; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D009865:Oocytes / Q000187:drug effects / Q000378:metabolism; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011985:Receptors, Serotonin / Q000187:drug effects / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution; D014982:Xenopus laevis / Q000235:genetics*
C505032:CFTR protein, human; D012333:RNA, Messenger; D011985:Receptors, Serotonin; D019005:Cystic Fibrosis Transmembrane Conductance Regulator; D004247:DNA; D017868:Cyclic AMP-Dependent Protein Kinases