title: The herpes simplex virus type 2 strain HG52 RL1 gene contains a 154 bp intron as predicted from sequence analysis.
abstract: The published sequence of the herpes simplex virus (HSV) type 2 strain HG52 neurovirulence gene RL1 indicated that, unlike the HSV-1 homologue, the gene contains a 154 bp intron. This intron contains six complete and one partial copy of a 19 bp repeat which encodes a stop codon; thus all three forward frames are blocked. By RT-PCR of poly(A)+ RNA, using primers on either side of the proposed intron, we have isolated a partial cDNA clone corresponding to the predicted spliced mRNA.
authors.affiliation: MRC Virology Unit, Institute of Virology, Glasgow, UK.
authors.forename: J E
authors.initials: JE
authors.lastname: Harland
authors.forename: S
authors.initials: S
authors.lastname: Bdour
authors.forename: S M
authors.initials: SM
authors.lastname: Brown
authors.forename: A R
authors.initials: AR
authors.lastname: MacLean
mesh_terms: D001483:Base Sequence; D004279:DNA, Viral; D005091:Exons; D005814:Genes, Viral; D018258:Herpesvirus 2, Human / Q000235:genetics* / Q000472:pathogenicity; D007438:Introns; D014764:Viral Proteins / Q000235:genetics*; D014774:Virulence / Q000235:genetics
chemical_list: D004279:DNA, Viral; D014764:Viral Proteins; C066469:gamma 34.5 protein, Human herpesvirus 1