RNA: RNA interactions in the large subunit ribosomal RNA of Euglena gracilis.
In Euglena gracilis, the cytoplasmic large subunit (LSU) rRNA is composed of 14 discrete small RNA species that must somehow interact in the functional ribosome. We have isolated native complexes of Euglena rRNA and show here that the largest of these complexes contains eight of the 14 LSU rRNA species. Several of these small rRNA species are able to associate in vitro to reform an isolated domain of LSU rRNA structure.
Smallman
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Canadian Institute for Advanced Research, Department of Biochemistry, Dalhousie University, Halifax, Nova Scotia, Canada.
Schnare
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Gray
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D000818:Animals; D001483:Base Sequence; D003593:Cytoplasm / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005056:Euglena gracilis / Q000235:genetics / Q000378:metabolism*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009690:Nucleic Acid Conformation; D012323:RNA Processing, Post-Transcriptional; D016053:RNA, Protozoan / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012335:RNA, Ribosomal / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012339:RNA, Ribosomal, 28S / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012340:RNA, Ribosomal, 5.8S / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012270:Ribosomes
D016053:RNA, Protozoan; D012335:RNA, Ribosomal; D012339:RNA, Ribosomal, 28S; D012340:RNA, Ribosomal, 5.8S; C065039:RNA, ribosomal, 25S