title: Modification of layered atelocollagen: enzymatic degradation and cytotoxicity evaluation.
abstract: Two kinds of layered atelocollagen materials cross-linked with hexamethylene diisocyanate (HMDIC), starch dialdehyde and glyoxal were enzymatically treated by bacterial collagenase. Evaluating collagenase digestion assay for these material showed progressive differences, particularly in the group of samples cross-linked with HMDIC. This should offer the possibility of programmed enzymatic degradation. These materials may be toxicologically acceptable as proven by the short-term test used for cytotoxicity evaluation.
authors.affiliation: Faculty of Chemical Technology, Slovak Technical University, Bratislava, Romania.
authors.forename: K
authors.initials: K
authors.lastname: Vizárová
authors.forename: D
authors.initials: D
authors.lastname: Bakos
authors.forename: M
authors.initials: M
authors.lastname: Rehákova
authors.forename: M
authors.initials: M
authors.lastname: Petríkova
authors.forename: E
authors.initials: E
authors.lastname: Panáková
authors.forename: J
authors.initials: J
authors.lastname: Koller
mesh_terms: D000595:Amino Acid Sequence; D001672:Biocompatible Materials / Q000737:chemistry* / Q000302:isolation & purification / Q000633:toxicity; D001673:Biodegradation, Environmental; D002460:Cell Line; D003094:Collagen / Q000737:chemistry* / Q000302:isolation & purification / Q000633:toxicity; D017364:Collagenases; D003432:Cross-Linking Reagents; D003485:Cyanates; D006037:Glyoxal; D006801:Humans; D017953:Isocyanates; D008422:Materials Testing; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000737:chemistry; D013213:Starch / Q000031:analogs & derivatives; D013379:Substrate Specificity
chemical_list: D001672:Biocompatible Materials; D003432:Cross-Linking Reagents; D003485:Cyanates; D017953:Isocyanates; D009842:Oligopeptides; C059821:atelocollagen; C015262:1,6-hexamethylene diisocyanate; D006037:Glyoxal; C031821:2-furanacryloyl-leucyl-glycyl-prolyl-alanine; D013213:Starch; D003094:Collagen; C012880:dialdehyde starch; D017364:Collagenases