title: Development of peripheral blood mononuclear cell response to mitogens in Japanese black newborn calves.
abstract: Development of peripheral blood mononuclear cells (PBMC) responses to phytohemagglutinin (PHA) and concanavalin A (Con A) in 5 Japanese Black newborn calves was examined by the MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide) assay until the day 28 after birth. The PHA responses of PBMC from calves aged from 0 to 7 days were less than those of PBMC from control adult cattle. While the difference of the Con A response of PBMC between newborn calves and adults was not significant statistically. These results suggest that the response of T cell to PHA may be insufficient at early stage after birth in Japanese Black.
authors.affiliation: Laboratory of Veterinary Internal Medicine, Faculty of Agriculture, Yamaguchi University, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Inokuma
authors.forename: T
authors.initials: T
authors.lastname: Yoshida
authors.forename: T
authors.initials: T
authors.lastname: Onishi
mesh_terms: D000375:Aging / Q000097:blood / Q000276:immunology; D000818:Animals; D000831:Animals, Newborn / Q000097:blood / Q000276:immunology*; D002417:Cattle / Q000097:blood / Q000276:immunology*; D003208:Concanavalin A / Q000494:pharmacology; D007564:Japan; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D008213:Lymphocyte Activation; D008934:Mitogens / Q000494:pharmacology*; D010835:Phytohemagglutinins / Q000494:pharmacology
chemical_list: D008934:Mitogens; D010835:Phytohemagglutinins; D003208:Concanavalin A