title: Delineation by use of specific monoclonal antibodies of the T-cell receptor and major histocompatibility complex interaction sites on the superantigen toxic shock syndrome toxin 1.
abstract: Murine monoclonal antibodies (MAbs) specific for toxic shock syndrome toxin 1 (TSST-1), a bacterial superantigen, showed the ability either to detect TSST-1 bound to histocompatibility locus antigen (HLA)-DR molecules or to inhibit TSST-1 binding to HLA-DR. A MAb capable of detecting DR-bound TSST-1 could also inhibit the toxin-induced activation of a T-cell receptor Vbeta15-expressing murine T-cell hybridoma. Alternatively, MAbs with specificity for the HLA-DR association site could present TSST-1 in vitro, stimulating CD4+ human T cells to proliferate. These functional activities correlated directly with with MAb specificity for HLA-DR versus T-cell receptor Vbeta interaction sites on TSST-1 as determined by reactivity with a panel of recombinant TSST-1 mutant molecules. Therefore, these MAbs discriminate the superantigen functional sites on the TSST-1 molecule and constitute reagents with the property of being potent modulators of the toxic activity of TSST-1.
authors.affiliation: Nexstar Pharmaceuticals, Inc., Lakewood Colorado, 80214, USA.
authors.forename: R
authors.initials: R
authors.lastname: Shimonkevitz
authors.forename: E
authors.initials: E
authors.lastname: Boen
authors.forename: S
authors.initials: S
authors.lastname: Malmstrom
authors.forename: E
authors.initials: E
authors.lastname: Brown
authors.forename: J M
authors.initials: JM
authors.lastname: Hurley
authors.forename: B L
authors.initials: BL
authors.lastname: Kotzin
authors.forename: M
authors.initials: M
authors.lastname: Matsumura
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D017951:Antigen Presentation; D001427:Bacterial Toxins; D001665:Binding Sites; D004768:Enterotoxins / Q000276:immunology* / Q000378:metabolism; D018604:Epitope Mapping; D006684:HLA-DR Antigens / Q000378:metabolism*; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D011948:Receptors, Antigen, T-Cell / Q000378:metabolism*; D013211:Staphylococcus aureus / Q000276:immunology*; D018089:Superantigens / Q000276:immunology* / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D001427:Bacterial Toxins; D004768:Enterotoxins; D006684:HLA-DR Antigens; D011948:Receptors, Antigen, T-Cell; D018089:Superantigens; C030107:enterotoxin F, Staphylococcal