title: Molecular epidemiological studies of Mycobacterium bovis infections in humans and animals in Sweden.
abstract: Forty-nine isolates of Mycobacterium bovis from humans and animals in Sweden were analyzed by restriction fragment length polymorphism (RFLP) patterns probed by the insertion element IS6110. Most isolates had patterns indicating the presence of only one or two genomic copies of the IS6110 insertion element. This simple type of pattern was found in all human isolates. In contrast, isolates from M. bovis infections in five herds of farmed deer in Sweden showed a specific RFLP pattern with seven bands, indicating seven copies of the IS6110 sequence. In 1958, Sweden was declared free from M. bovis in cattle. However, in 1987, M. bovis was reintroduced with imported farmed deer, and since 1991, 11 outbreaks in deer herds, but not in other livestock or wildlife, have been diagnosed. Continued RFLP studies of the new Swedish M. bovis isolates can reveal possible transmission of this deer strain to other animals or humans.
authors.affiliation: Department of Bacteriology, Swedish Institute for Infectious Disease Control, Stockholm, Sweden.
authors.forename: R
authors.initials: R
authors.lastname: Szewzyk
authors.forename: S B
authors.initials: SB
authors.lastname: Svenson
authors.forename: S E
authors.initials: SE
authors.lastname: Hoffner
authors.forename: G
authors.initials: G
authors.lastname: Bölske
authors.forename: H
authors.initials: H
authors.lastname: Wahlström
authors.forename: L
authors.initials: L
authors.lastname: Englund
authors.forename: A
authors.initials: A
authors.lastname: Engvall
authors.forename: G
authors.initials: G
authors.lastname: Källenius
mesh_terms: D000818:Animals; D001483:Base Sequence; D002417:Cattle; D017931:DNA Primers / Q000235:genetics; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000235:genetics; D003670:Deer; D004196:Disease Outbreaks / Q000662:veterinary*; D006801:Humans; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D009163:Mycobacterium bovis / Q000235:genetics* / Q000302:isolation & purification*; D012150:Polymorphism, Restriction Fragment Length; D013045:Species Specificity; D013548:Sweden / Q000453:epidemiology; D014376:Tuberculosis / Q000453:epidemiology* / Q000382:microbiology / Q000662:veterinary*; D014380:Tuberculosis, Bovine / Q000453:epidemiology / Q000382:microbiology
chemical_list: D017931:DNA Primers; D004251:DNA Transposable Elements; D004269:DNA, Bacterial