Determination of DDT and related compounds in blood samples from agricultural workers.
An analytical method combining a solid-phase (C18) clean-up and GC-electron-capture detection using a capillary column, was implemented to determine p,p'-DDT and its metabolites (p,p'-DDD and p,p'-DDE), as well as other organochlorine pesticides in whole blood samples from 30 farmers and 24 non-occupationally exposed workers. The average concentrations for the quantified pesticides, p,p'-DDT, p,p'-DDD and p,p'-DDE, were 0.9, 1.5 and 8.0 micrograms/l whole blood for exposed workers and 0.3, 0.5 and 3.3 micrograms/l for unexposed workers, respectively. GC-MS was used to confirm the identity of the pesticides found. Solid-phase extraction and the protocol used give a cleaner analytical matrix, not only improving sensitivity and resolution, but also allowing analyses with smaller blood samples as compared to other methods.
Guardino
X
X
Instito Nacional de Seguridad e Higiene en el Trabajo, Centro Nacional de Condiciones de Trabajo, Barcelona, Spain.
Serra
C
C
Obiols
J
J
Rosell
M G
MG
Berenguer
M J
MJ
López
F
F
Brosa
J
J
D000383:Agriculture; D002849:Chromatography, Gas / Q000379:methods*; D003634:DDT / Q000097:blood*; D003633:Dichlorodiphenyl Dichloroethylene / Q000097:blood; D003632:Dichlorodiphenyldichloroethane / Q000097:blood; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007306:Insecticides / Q000097:blood*; D016273:Occupational Exposure
D007306:Insecticides; D003633:Dichlorodiphenyl Dichloroethylene; D003634:DDT; D003632:Dichlorodiphenyldichloroethane