Cellular induction mechanism of CD8+ suppressor T cells by DMBA and TPA: formation of CD4+ suppressor-inducer T cells.
We investigated the cellular induction mechanism of antigen-nonspecific CD8+ T suppressor cells which suppressed delayed-type hypersensitivity to sheep red blood cells, by treating BALB/c mice with 7,12-dimethylbenz[a]anthracene (DMBA), a tumor initiator, and 12-O-tetradecanoylphorbol 13-acetate (TPA), a tumor promoter. Macrophages were activated by painting with 400 nmol of DMBA on mice. And the macrophages plus TPA induced CD4+ T suppressor inducer cells in the mice spleens. These cells were efficient at inducing CD8+ T suppressor-effector cells. When 8 nmol of TPA was painted daily on mice for 3 days following the treatment with 400 nmol of DMBA, or when spleen cells from mice pretreated with 400 nmol of DMBA were cultured in 32 nmol/5 ml of TPA for 3 days, inducer cells were formed in the spleen. Both T suppressor-inducer cells and macrophages from mice treated with DMBA were shown to act with the formation of soluble factors, which may be different from IL-10.
Negoro
T
T
Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Teikyo University, Kanagawa, Japan.
Iinuma
F
F
Watanabe
M
M
D015127:9,10-Dimethyl-1,2-benzanthracene / Q000494:pharmacology*; D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D002273:Carcinogens / Q000494:pharmacology*; D006968:Hypersensitivity, Delayed / Q000276:immunology; D007111:Immunity, Cellular / Q000276:immunology; D007371:Interferon-gamma / Q000096:biosynthesis; D008213:Lymphocyte Activation; D008222:Lymphokines / Q000502:physiology; D008264:Macrophages / Q000276:immunology; D008297:Male; D051379:Mice; D008806:Mice, Inbred AKR; D008807:Mice, Inbred BALB C; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D018417:Th1 Cells / Q000276:immunology
D002273:Carcinogens; D008222:Lymphokines; D015127:9,10-Dimethyl-1,2-benzanthracene; D007371:Interferon-gamma; D013755:Tetradecanoylphorbol Acetate