title: Direct visualization of A-, P-, and E-site transfer RNAs in the Escherichia coli ribosome.
abstract: Transfer RNA (tRNA) molecules play a crucial role in protein biosynthesis in all organisms. Their interactions with ribosomes mediate the translation of genetic messages into polypeptides. Three tRNAs bound to the Escherichia coli 70S ribosome were visualized directly with cryoelectron microscopy and three-dimensional reconstruction. The detailed arrangement of A- and P-site tRNAs inferred from this study allows localization of the sites for anticodon interaction and peptide bond formation on the ribosome.
authors.affiliation: Wadsworth Center, New York State Department of Health, Albany 12201-0509, USA.
authors.forename: R K
authors.initials: RK
authors.lastname: Agrawal
authors.forename: P
authors.initials: P
authors.lastname: Penczek
authors.forename: R A
authors.initials: RA
authors.lastname: Grassucci
authors.forename: Y
authors.initials: Y
authors.lastname: Li
authors.forename: A
authors.initials: A
authors.lastname: Leith
authors.forename: K H
authors.initials: KH
authors.lastname: Nierhaus
authors.forename: J
authors.initials: J
authors.lastname: Frank
mesh_terms: D000926:Anticodon; D001665:Binding Sites; D003062:Codon; D004926:Escherichia coli / Q000378:metabolism*; D007091:Image Processing, Computer-Assisted; D008854:Microscopy, Electron; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D012329:RNA, Bacterial / Q000737:chemistry / Q000378:metabolism; D012343:RNA, Transfer / Q000737:chemistry* / Q000378:metabolism; D012346:RNA, Transfer, Amino Acyl / Q000737:chemistry* / Q000378:metabolism; D012360:RNA, Transfer, Phe / Q000737:chemistry* / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism*
chemical_list: D000926:Anticodon; D003062:Codon; D012329:RNA, Bacterial; D012346:RNA, Transfer, Amino Acyl; D012360:RNA, Transfer, Phe; C019037:tRNA, peptidyl-; D012343:RNA, Transfer