Methanol extracts LPS from deep rough bacteria.
Several rough strains of Escherichia coli, Salmonella enterica and Proteus mirabilis were cultivated in the presence of (14C)acetate, which incorporated into their phospholipids and lipopolysaccharide. Phospholipids were removed from the cells with ethanol extraction. However, as shown by thin layer chromatography, methanol additionally extracted remarkable quantities of lipopolysaccharide from deep rough strains, but not from bacteria containing complete or nearly complete core.
Nurminen
M
M
Department of Bacteriology and Immunology, University of Helsinki, Finland.
Vaara
M
M
D002855:Chromatography, Thin Layer; D004926:Escherichia coli / Q000737:chemistry* / Q000235:genetics / Q000254:growth & development / Q000276:immunology; D005838:Genotype; D008070:Lipopolysaccharides / Q000302:isolation & purification*; D000432:Methanol; D011513:Proteus mirabilis / Q000737:chemistry* / Q000235:genetics / Q000254:growth & development / Q000276:immunology; D012475:Salmonella / Q000737:chemistry* / Q000235:genetics / Q000254:growth & development / Q000276:immunology; D012997:Solvents; D013045:Species Specificity
D008070:Lipopolysaccharides; D012997:Solvents; D000432:Methanol