Anti-DNA antibody derived from a systemic lupus erythematosus (SLE) patient forms histone-DNA-anti-DNA complexes that bind to rat glomeruli in vivo.
Histone can mediate the binding of both free DNA and DNA complexed to anti-DNA antibody to the glomerular capillary wall. We tested whether performed histone-DNA-anti-DNA immune complexes (IC) could bind to the glomerular capillary wall. The immune complex, generated with anti-DNA antibody derived from an SLE patient and excess of 125I-DNA followed by digestion with DNase, was mixed with histones. The complex containing 4 micrograms DNA was injected via the aorta into the left kidney of rats. At 15 min, 1-3% of the histone-DNA-anti-DNA antibody complex bound (measured as 125I-DNA), when histone was omitted less than 0-1% of the DNA-anti-DNA antibody complex bound. By immunofluorescence human immunoglobulins and histones, representing the IC, could be observed in a capillary pattern; but no complement deposition was detected. Electron microscopy revealed discrete, electron dense deposits in a subendothelial, subepithelial and mesangial localization at 15 min. These results provide direct evidence that antibodies from serum of SLE patients can form soluble histone-DNA-anti-DNA immune complexes that bind to the glomerular capillary wall in vivo.
Morioka
T
T
Abeteilung Immunologie, Institut für Medizinische Mikrobiologie und Hygiene, Freiburg, Germany.
Fujigaki
Y
Y
Batsford
S R
SR
Woitas
R
R
Oite
T
T
Shimizu
F
F
Vogt
A
A
D000818:Animals; D000974:Antibodies, Antinuclear / Q000276:immunology*; D000936:Antigen-Antibody Complex / Q000378:metabolism; D004247:DNA / Q000276:immunology; D003852:Deoxyribonucleoproteins / Q000276:immunology; D006657:Histones / Q000276:immunology*; D006801:Humans; D007678:Kidney Glomerulus / Q000276:immunology*; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*; D008297:Male; D051381:Rats; D017208:Rats, Wistar
D000974:Antibodies, Antinuclear; D000936:Antigen-Antibody Complex; D003852:Deoxyribonucleoproteins; D006657:Histones; D004247:DNA