A region of Haemophilus influenzae Rd hybridizes with repetitive human DNA.
A 17.7 kbp EcoR1 fragment of Haemophilus influenzae Rd (wild-type strain) DNA containing repeats has been cloned together with the DNA repair gene uvr3. The region containing repetitive DNA was subcloned on a 13 kbp EcoR1 fragment (pHi13). The insert hybridizes with restriction enzyme digests of H. influenzae Rd and H. parainfluenzae and exhibits a repetitive pattern. Discrete bands superimposed upon a polydisperse background were observed when pHi13 hybridizes with restriction digests of human DNA. The hybridization patterns indicate that H. parainfluenzae and human DNA contain cross-hybridizing sequences. The repeats of pHi13 are present on a 2.6 kbp PvuII fragment.
Mody
R B
RB
Molecular Biology & Agriculture Div., Bhabha Atomic Research Centre, Trombay, Bombay, India.
Joshi
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VP
D004247:DNA / Q000235:genetics; D004260:DNA Repair / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D006193:Haemophilus influenzae / Q000235:genetics*; D006801:Humans; D009693:Nucleic Acid Hybridization; D011836:Radiation Tolerance / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid; D014466:Ultraviolet Rays
D004269:DNA, Bacterial; D004247:DNA