Campylobacter fetus subspecies fetus septicaemia: SDS-PAGE as an aid to speciation.
We describe a case of bacteraemia due to an atypical strain of Campylobacter fetus ssp. fetus. Conventional biochemical and phenotypic characterisation was unhelpful but whole cell protein profiles obtained by means of sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) allowed us to identify this strain.
Howe
R A
RA
Department of Microbiology, Royal Hallamshire Hospital, Sheffield, UK.
Clarke
T
T
Wilcox
M H
MH
Vandamme
P
P
Spencer
R C
RC
D016470:Bacteremia / Q000382:microbiology*; D001426:Bacterial Proteins / Q000302:isolation & purification; D002169:Campylobacter Infections / Q000382:microbiology*; D002168:Campylobacter fetus / Q000145:classification* / Q000302:isolation & purification; D002648:Child; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D006801:Humans; D008297:Male; D012967:Sodium Dodecyl Sulfate; D013045:Species Specificity; D013501:Surface-Active Agents
D001426:Bacterial Proteins; D013501:Surface-Active Agents; D012967:Sodium Dodecyl Sulfate