title: Hantaan virus persistently infects marmoset B-lymphoblastoid cell line.
abstract: To investigate lymphoid cell Hantavirus (HV) interaction, we examined the replication of the HV strains, Hantaan 76-118 (HTN), SR-11, and B-1 with the B95a, LYM-1, CGM1, JM, Jurkat, MOLT-4, AT(L)5KY, BW5147, L1210, and Vero/E6 cells. HV-growth was determined by immunofluorescence and focus-forming assays. LYM-1 and Vero/E6 supported the growth of all HV strains, and B95a only HTN, while remaining cells did not sustain these strains. This is the first report to indicate the HV-replication in lymphoid cell lines. B95a and LYM-1 cells may be useful for revealing the mechanism of HV-infection in animals.
authors.affiliation: Laboratory Animal Center for Biomedical Research, Nagasaki University School of Medicine, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Ohsawa
authors.forename: Y
authors.initials: Y
authors.lastname: Watanabe
authors.forename: H
authors.initials: H
authors.lastname: Sato
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000473:pathology / Q000821:virology*; D002144:Callithrix; D002460:Cell Line; D002522:Chlorocebus aethiops; D005455:Fluorescent Antibody Technique; D018063:Hantaan virus / Q000145:classification / Q000302:isolation & purification / Q000502:physiology*; D006480:Hemorrhagic Fever with Renal Syndrome / Q000209:etiology / Q000473:pathology / Q000821:virology; D006801:Humans; D014709:Vero Cells; D014779:Virus Replication / Q000502:physiology