The isolation and characterisation of a gene encoding superoxide dismutase from Bradyrhizobium sp. (Parasponia) strain ANU289.
Bradyrhizobium sp. (Parasponia) strain ANU289 expresses a single Mn-SOD in both the vegetative and symbiotic states. A 500 bp sod-homologous sequence was amplified from genomic DNA of strain ANU289 using PCR. A 1.3 kb SalI fragment was subsequently cloned which contained an ORF, sodA, encoding a 23 Kd protein. This putative SOD shares considerable homology with other Mn-SODs and analysis of the sodA sequence predicts that it is expressed. A lacZ-sodA fusion complemented the SOD-deficiency of E. coli QC779 and resulted in the expression of SOD activity in both mutant and wild type E. coli. We conclude that sodA encodes the Mn-SOD of strain ANU289.
Thygesen
P W
PW
Division of Biochemistry and Molecular Biology, Faculty of Science, Australian National University, Canberra ACT, Australia.
Whelan
J
J
Morell
M K
MK
Day
D A
DA
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000378:metabolism; D005815:Genetic Code; D016680:Genome, Bacterial; D007527:Isoenzymes / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012230:Rhizobiaceae / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D013482:Superoxide Dismutase / Q000235:genetics*; D014197:Trees
D007527:Isoenzymes; D013482:Superoxide Dismutase