title: A permease-oxidase complex involved in high-affinity iron uptake in yeast.
abstract: Iron must cross biological membranes to reach essential intracellular enzymes. Two proteins in the plasma membrane of yeast--a multicopper oxidase, encoded by the FET3 gene, and a permease, encoded by the FTR1 gene--were shown to mediate high-affinity iron uptake. FET3 expression was required for FTR1 protein to be transported to the plasma membrane. FTR1 expression was required for apo-FET3 protein to be loaded with copper and thus acquire oxidase activity. FTR1 protein also played a direct role in iron transport. Mutations in a conserved sequence motif of FTR1 specifically blocked iron transport.
authors.affiliation: Cell Biology and Metabolism Branch, National Institutes of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA.
authors.forename: R
authors.initials: R
authors.lastname: Stearman
authors.forename: D S
authors.initials: DS
authors.lastname: Yuan
authors.forename: Y
authors.initials: Y
authors.lastname: Yamaguchi-Iwai
authors.forename: R D
authors.initials: RD
authors.lastname: Klausner
authors.forename: A
authors.initials: A
authors.lastname: Dancis
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D001692:Biological Transport; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D002570:Ceruloplasmin; D003300:Copper / Q000378:metabolism / Q000494:pharmacology; D004721:Endoplasmic Reticulum / Q000378:metabolism; D005290:Ferric Compounds / Q000378:metabolism; D005293:Ferritins / Q000737:chemistry / Q000378:metabolism; D005296:Ferrous Compounds / Q000378:metabolism; D005800:Genes, Fungal; D006056:Golgi Apparatus / Q000378:metabolism; D007501:Iron / Q000378:metabolism*; D026901:Membrane Transport Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D008954:Models, Biological; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000378:metabolism*; D009154:Mutation; D016366:Open Reading Frames; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins; D014170:Transformation, Genetic
chemical_list: D002352:Carrier Proteins; C098723:FTR1 protein, S cerevisiae; D005290:Ferric Compounds; D005296:Ferrous Compounds; D026901:Membrane Transport Proteins; D009097:Multienzyme Complexes; D029701:Saccharomyces cerevisiae Proteins; D003300:Copper; D005293:Ferritins; D007501:Iron; D010088:Oxidoreductases; D002570:Ceruloplasmin; C085513:FET3 protein, S cerevisiae