title: Electron spectroscopic imaging of encapsidated DNA in vaccinia virus.
abstract: We have used electron spectroscopic imaging to locate the phosphorus in vaccinia DNA in situ in unstained, ultrathin sections of virions. The phosphorus of the DNA backbone appeared to form a halo on the core periphery surrounding a phosphorus-impoverished central element. These results constrain models for how DNA could be packaged into mature vaccinia particles.
authors.affiliation: Department of Molecular Biology and Genetics, University of Guelph, Canada.
authors.forename: G
authors.initials: G
authors.lastname: Harauz
authors.forename: D H
authors.initials: DH
authors.lastname: Evans
authors.forename: D R
authors.initials: DR
authors.lastname: Beniac
authors.forename: A L
authors.initials: AL
authors.lastname: Arsenault
authors.forename: B
authors.initials: B
authors.lastname: Rutherford
authors.forename: F P
authors.initials: FP
authors.lastname: Ottensmeyer
mesh_terms: D004279:DNA, Viral / Q000032:analysis / Q000737:chemistry / Q000648:ultrastructure*; D004577:Electron Probe Microanalysis; D008854:Microscopy, Electron; D010758:Phosphorus / Q000032:analysis; D014616:Vaccinia virus / Q000235:genetics* / Q000648:ultrastructure; D014771:Virion / Q000235:genetics / Q000648:ultrastructure
chemical_list: D004279:DNA, Viral; D010758:Phosphorus