Novel nuclear ribonucleoprotein structural components in the dormouse adrenal cortex during hibernation.
Adrenocortical cell nuclei of the dormouse Muscardinus avellanarius were investigated by electron microscopic immunocytochemistry in hibernating, arousing and euthermic individuals. While the basic structural constituents of the cell nucleus did not significantly modify in the three groups, novel structural components were found in nuclei of hibernating dormice. Lattice-like bodies (LBs), clustered granules (CGs), fibrogranular material (FGM) and granules associated with bundles of nucleoplasmic fibrils (NF) all contained ribonucleoproteins (RNPs), as shown by labeling with anti-snRNP (small nuclear RNP), anti-m3G-capped RNA and anti-hnRNP (heterogeneous nuclear RNP) antibodies. Moreover, the FGM also showed immunoreactivity for the proliferation associated nuclear antigen (PANA) and the non-snRNP splicing factor SC-35. All these nuclear structural components disappeared early during arousal and were not found in euthermic animals. These novel RNP-containing structures, which have not been observed in other tissues investigated so far in the same animal model, could represent storage and/or processing sites for pre-mRNA during the extreme metabolic condition of hibernation, to be quickly released upon arousal. NFs, which had been sometimes found devoid of associated granules in nuclei of brown adipose tissue from hi-bernating dormice, were present in much higher amounts in adrenocortical cell nuclei; they do not contain RNPs and their role remains to be elucidated. The possible roles of these structures are discussed in the frame of current knowledge of morpho-functional relationships in the cell nucleus.
Malatesta
M
M
Centre of Electron Microscopy, University of Lausanne, 27 Bugnon, CH-1005 Lausanne, Switzerland.
Zancanaro
C
C
Tamburini
M
M
Martin
T E
TE
Fu
X D
XD
Vogel
P
P
Fagan
S
S
D000302:Adrenal Cortex / Q000648:ultrastructure*; D000818:Animals; D000906:Antibodies; D002467:Cell Nucleus / Q000648:ultrastructure; D002843:Chromatin / Q000648:ultrastructure; D006605:Hibernation / Q000502:physiology*; D007150:Immunohistochemistry / Q000379:methods; D008854:Microscopy, Electron; D009687:Nuclear Proteins / Q000276:immunology; D018809:Proliferating Cell Nuclear Antigen / Q000276:immunology; D012315:RNA Caps; D012261:Ribonucleoproteins / Q000737:chemistry*; D017411:Ribonucleoproteins, Small Nuclear / Q000737:chemistry / Q000276:immunology; D012377:Rodentia / Q000502:physiology*
D000906:Antibodies; D002843:Chromatin; D009687:Nuclear Proteins; D018809:Proliferating Cell Nuclear Antigen; D012315:RNA Caps; D012261:Ribonucleoproteins; D017411:Ribonucleoproteins, Small Nuclear