Is aging related to cell signalling alterations in amphibian oocytes?
The storage of Bufo arenarum oocytes decreased their ability to become fertilized "in vitro". The stimulation with carbachol of "young oocytes" showed a persistent hydrolysis with phosphatidylinositol 4,5 diphosphate (PIP2) while in "aged oocytes" both phosphatidylinositol 4 phosphate (PIP) and PIP2 were hydrolyzed at a non-significant level. These results and the lower 32P-phosphoinositide levels found in "aged oocytes" at time of stimulation agree with diminished phosphoinositide kinase and phospholipase C activities, as the consequence of a non-specific phosphoinositide hydrolysis probably occurring during storage.
Fonovich de Schroeder
T M
TM
Libiquima, Facultad de Ingeniería, Universidad Nacional del Comahue, Buenos Aires, Neuquén, Argentina.
de D'Angelo
A M
AM
D000704:Analysis of Variance; D000818:Animals; D002022:Bufo arenarum / Q000502:physiology*; D002217:Carbachol / Q000494:pharmacology; D002450:Cell Communication / Q000187:drug effects / Q000502:physiology*; D016922:Cellular Senescence / Q000502:physiology; D005306:Fertilization / Q000187:drug effects / Q000502:physiology*; D018721:Muscarinic Agonists / Q000494:pharmacology; D009865:Oocytes / Q000187:drug effects / Q000502:physiology*; D010716:Phosphatidylinositols; D013997:Time Factors
D018721:Muscarinic Agonists; D010716:Phosphatidylinositols; D002217:Carbachol