Immunopathological studies on guinea pigs experimentally infected with Egyptian strain of Leishmania d. infantum.
Forty Guinea pigs were experimentally infected with Egyptian strain of Leishmania d. infantum. At necropsy, materials for histopathological studies were taken from spleen, liver & lymph nodes and for smears from the same organs and bone marrow as well. Also, serum samples were examined for the elevation of immunoglobulins (IgG., IgM. & IgA.). Amastigotes of Leishmania parasites were demonstrated in the kupffer cells in the liver and in the macrophages in the spleen and lymph nodes as well as the smears of the bone marrow. The immunoglobulins showed varying levels. The IgG increased with the decrease in number of tissue amastigotes. The splenic materials were positive in all the experimentally infected Guinea pigs which means that splenic puncture is the best method of diagnosing VL. However, it may be dangerous in soft spleen. The results were photographed and discussed.
Eesa
T M
TM
Department of Pathology, Faculty of Medicine, University of Tanta, Egypt.
el Tatawi
F
F
Mokhtar
M A
MA
Khalil
N M
NM
Morsy
T A
TA
D000818:Animals; D001853:Bone Marrow / Q000469:parasitology / Q000473:pathology; D004534:Egypt; D005260:Female; D006168:Guinea Pigs; D007070:Immunoglobulin A / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D007728:Kupffer Cells / Q000469:parasitology / Q000473:pathology; D018314:Leishmania infantum / Q000302:isolation & purification; D007898:Leishmaniasis, Visceral / Q000276:immunology* / Q000473:pathology*; D008099:Liver / Q000469:parasitology / Q000473:pathology; D008198:Lymph Nodes / Q000469:parasitology / Q000473:pathology; D008297:Male; D013154:Spleen / Q000469:parasitology / Q000473:pathology
D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M