title: Effect of cytokines on sperm motility and ionophore-stimulated acrosome reaction.
abstract: The effects of soluble products from leukocytes (WBC) on sperm motility and calcium ionophore-stimulated acrosome reaction (AR) were examined. Supernatants of mononuclear WBC, isolated from peripheral blood and stimulated by the lectins Phytolacca americana (pokeweed mitogen) or concanavalin A, caused a weak but significant inhibition of progressive sperm motility. The recombinant cytokine interferon-gamma (IFN-gamma) in high concentrations inhibited motility of sperm from 5 of 8 donors tested. The recombinant cytokines tumor necrosis factor-alpha (TNF-alpha) and interleukin-8 (IL-8) did not show any effect on sperm motility. Reactive oxygen species (ROS) produced by stimulation of polymorphonuclear WBC with a phorbol ester (PMA) tended to inhibit sperm motility. Neither supernatants from lectin-stimulated mononuclear WBC, IFN-gamma, TNF-alpha, IL-8, nor ROS showed any significant effect on the ionophore-challenged AR.
authors.affiliation: Department of Gynecology and Obstetrics, University Hospital of Aarhus, Denmark.
authors.forename: J
authors.initials: J
authors.lastname: Fedder
authors.forename: S
authors.initials: S
authors.lastname: Ellerman-Eriksen
mesh_terms: D000177:Acrosome / Q000187:drug effects* / Q000502:physiology*; D002273:Carcinogens / Q000494:pharmacology; D003208:Concanavalin A / Q000494:pharmacology; D016207:Cytokines / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology; D016209:Interleukin-8 / Q000494:pharmacology; D007476:Ionophores / Q000494:pharmacology*; D037102:Lectins / Q000494:pharmacology; D007962:Leukocytes / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D008297:Male; D011043:Pokeweed Mitogens / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000378:metabolism; D011994:Recombinant Proteins / Q000494:pharmacology; D013081:Sperm Motility / Q000187:drug effects* / Q000502:physiology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
chemical_list: D002273:Carcinogens; D016207:Cytokines; D016209:Interleukin-8; D007476:Ionophores; D037102:Lectins; D011043:Pokeweed Mitogens; D017382:Reactive Oxygen Species; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D003208:Concanavalin A; D007371:Interferon-gamma; D013755:Tetradecanoylphorbol Acetate