[A new site-specific endonuclease from Bacillus species ST5].
A site-specific endonuclease R.BspST5I has been isolated in a functionally pure state from the thermophilic strain of Bacillus species ST5. The enzyme recognizes sequence 5'-GCATC-3' on the DNA and splits it at a distance of five nucleotides from the 3'-end of the recognition site as well as at distances of nine or ten nucleotides at the complementary filament depending on the hydrolyzed sequence of the DNA. The enzyme is a isomer of endonuclease SfaNI from Streptococcus faecalis ND547.
Zelinskaia
N V
NV
Matvienko
N N
NN
Zheleznaia
L A
LA
Matvienko
N I
NI
D001407:Bacillus / Q000201:enzymology*; D001483:Base Sequence; D002850:Chromatography, Gel; D004247:DNA / Q000378:metabolism; D004262:DNA Restriction Enzymes / Q000302:isolation & purification* / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D008969:Molecular Sequence Data
D004247:DNA; D004262:DNA Restriction Enzymes