Detection of antibodies to 65 KD heat shock protein and to human superoxide dismutase in autoimmune hepatitis-molecular mimicry between 65 KD heat shock protein and superoxide dismutase.
The antibody to 65 KD mycobacterial heat shock protein (HSP65) and antibody to human superoxide dismutase (H-SOD) were measured by ELISA in patients with autoimmune hepatitis (AIH), and results were compared with those of patients with chronic active hepatitis C (CAH-C) or systemic lupus erythematosus (SLE) and normal subjects (NS). Patients with AIH had significantly higher OD values of anti-HSP65 antibody and anti-H-SOD antibody compared with those of patients with CAH-C or SLE and NS. OD values of anti-HSP65 antibody were correlated with those of anti-SOD antibody. Affinity-purified anti-SOD antibody reacted with HSP65. Analysis of the amino acid sequence of human SOD showed that 7 segments, corresponding to r to 25 amino acid residues, exhibited 50 to 71% homology with that of my mycobacterial HSP65.
Miyata
M
M
Department of Internal Medicine II, Fukushima Medical College, JAPAN.
Kogure
A
A
Sato
H
H
Kodama
E
E
Watanabe
H
H
Ohira
H
H
Kuroda
M
M
Takagi
T
T
Sato
Y
Y
Kasukawa
R
R
D000595:Amino Acid Sequence; D001323:Autoantibodies / Q000097:blood*; D001327:Autoimmune Diseases / Q000175:diagnosis / Q000276:immunology*; D001426:Bacterial Proteins; D018834:Chaperonin 60 / Q000276:immunology; D018833:Chaperonins / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006505:Hepatitis / Q000175:diagnosis / Q000276:immunology*; D006801:Humans; D018716:Molecular Mimicry; D008969:Molecular Sequence Data; D013482:Superoxide Dismutase / Q000235:genetics / Q000276:immunology*
D001323:Autoantibodies; D001426:Bacterial Proteins; D018834:Chaperonin 60; C074119:heat-shock protein 65, Mycobacterium; D013482:Superoxide Dismutase; D018833:Chaperonins