title: Isolation and description of carbazole-degrading bacteria.
abstract: Carbazole is a nitrogen heterocyclic compound associated with fossil fuels and their products. Enrichment cultures were established to isolate bacteria able to degrade carbazole and a plate assay was developed to select carbazole-degrading microorganisms from the enrichments. Three different bacterial isolates capable of mineralizing carbazole were obtained. No intermediates of carbazole degradation were detected. The bacteria had a limited substrate specificity; all used benzoate for growth but were unable to utilize the analogues of carbazole, fluorene, or dibenzothiophene.
authors.affiliation: Department of Biological Sciences, University of Waikato, Hamilton, New Zealand.
authors.forename: J
authors.initials: J
authors.lastname: Shotbolt-Brown
authors.forename: D W
authors.initials: DW
authors.lastname: Hunter
authors.forename: J
authors.initials: J
authors.lastname: Aislabie
mesh_terms: D001431:Bacteriological Techniques; D001565:Benzoates / Q000378:metabolism; D019817:Benzoic Acid; D001673:Biodegradation, Environmental; D019117:Burkholderia / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*; D002227:Carbazoles / Q000378:metabolism*; D004785:Environmental Pollutants / Q000378:metabolism*; D005449:Fluorenes / Q000378:metabolism; D011549:Pseudomonas / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*; D012641:Selection, Genetic; D012988:Soil Microbiology; D013379:Substrate Specificity; D014974:Xanthomonas / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D001565:Benzoates; D002227:Carbazoles; D004785:Environmental Pollutants; D005449:Fluorenes; C041514:carbazole; C041509:fluorene; D019817:Benzoic Acid