title: Leukocyte function-associated antigen-1-dependent adhesion of rat ascites hepatoma AH66F to mesentery-derived mesothelial cells.
abstract: Rat ascites hepatoma AH66F cells adhered better than AH130 cells to mesentery-derived mesothelial cells (M-cells), though both cells secreted Mr 92,000 matrix metalloproteinase on a gelatin zymogram with similar activity. AH66F cells expressed leukocyte function-associated antigen-1 (LFA-1) and intercellular adhesion molecule-1 (ICAM-1), a ligand of LFA-1, on the cell surface, while AH130 cells had ICAM-1 alone. The adhesion of M-cells of AH66F cells was inhibited to the adhesion level of AH130 cells by anti-rat LFA-1 alpha -and/or beta-chain monoclonal antibody (mAb) and also by anti-rat ICAM-1 mAb. This is the first report to show the LFA-1-dependent adhesion of cells other than leukocytes, because AH66F cells did not express CD45, T cell-alpha beta receptor or Cd11b/c (Mac-1/p150,95). These results indicate that a part of the adhesion of AH66F cells to M-cells is due to LFA-1/ICAM-1 interaction, and we suggest that this characteristic feature of AH66F cells may be related to the malignant properties.
authors.affiliation: Research Laboratory for Development of Medicine, Faculty of Pharmaceutical Sciences, Hokuriku University.
authors.forename: M
authors.initials: M
authors.lastname: Nomura
authors.forename: H
authors.initials: H
authors.lastname: Yamamoto
authors.forename: N
authors.initials: N
authors.lastname: Sugiura
authors.forename: K
authors.initials: K
authors.lastname: Kuroda
authors.forename: H
authors.initials: H
authors.lastname: Kawaguchi
authors.forename: K
authors.initials: K
authors.lastname: Miyamoto
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000502:physiology; D002478:Cells, Cultured; D017364:Collagenases / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry; D015151:Immunoblotting; D018799:Intercellular Adhesion Molecule-1 / Q000502:physiology; D008114:Liver Neoplasms, Experimental / Q000473:pathology*; D016169:Lymphocyte Function-Associated Antigen-1 / Q000502:physiology*; D020780:Matrix Metalloproteinase 9; D008643:Mesentery / Q000166:cytology; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D016169:Lymphocyte Function-Associated Antigen-1; D018799:Intercellular Adhesion Molecule-1; D017364:Collagenases; D020780:Matrix Metalloproteinase 9