A biosensor concept based on imaging ellipsometry for visualization of biomolecular interactions.
A new type of optical biosensor system is proposed and its application to detection of antigen-antibody complexes on a silicon substrate is demonstrated. The biosensor system is utilizing specificities of biomolecular interactions in combination with protein patterned surfaces. Visualization of the thickness distributions of thin layers (protein patterns) on a surface is achieved by using imaging ellipsometry. This technique uses a CCD camera whereby an ellipsometric analysis of a large surface can be made. The biosensor system has the advantages of high spatial resolution, fast data acquisition, and simplicity in use.
Jin
G
G
Department of Physics and Measurement Technology, Linköping University, Sweden.
Tengvall
P
P
Lundström
I
I
Arwin
H
H
D000327:Adsorption; D000936:Antigen-Antibody Complex / Q000032:analysis* / Q000378:metabolism; D015374:Biosensing Techniques; D005340:Fibrinogen / Q000276:immunology / Q000378:metabolism; D007074:Immunoglobulin G / Q000276:immunology / Q000378:metabolism; D008027:Light; D012709:Serum Albumin / Q000276:immunology / Q000378:metabolism; D013499:Surface Properties; D014741:Video Recording
D000936:Antigen-Antibody Complex; D007074:Immunoglobulin G; D012709:Serum Albumin; D005340:Fibrinogen