[The effects of synthetic oligopeptides on progesterone production in corpus luteum cell of rat and their mechanism of action].
The effects of a series of synthetic oligopeptides on progesterone production by rat CL cells were compared and their mechanism of actions was studied in vitro. The ones with inhibitory actions were characterized by carrying positive charge in the medium of pH 7.3-7.5 and with intermolecular linkage. A preliminary survey of the effect of the active oligopeptides on signal systems showed: (1)GY and YG inhibited PLC system; (2)GY and GSK reduced hCG-induced progesterone production in CL cells probably by regulating cellular Ca2+ concentration; (3) GSK decreased TPK activity and GYK increased it in hCG treated CL cell though both of them were inhibitory on progesterone production. GSK also stimulated PKC and supressed PKA activity in CL cells. The anti-progesterone effect of oligopeptides so far synthesized may influence either PKA or PKC or TPK systems. The mechanism of action at the molecular level is quite complicated.
Wang
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D000818:Animals; D003280:Contraceptives, Oral, Synthetic / Q000494:pharmacology*; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism; D005260:Female; D008184:Luteal Cells / Q000201:enzymology / Q000378:metabolism*; D009842:Oligopeptides / Q000494:pharmacology*; D011374:Progesterone / Q000096:biosynthesis*; D011493:Protein Kinase C / Q000378:metabolism; D051381:Rats
D003280:Contraceptives, Oral, Synthetic; D009842:Oligopeptides; D011374:Progesterone; D017868:Cyclic AMP-Dependent Protein Kinases; D011493:Protein Kinase C