Immunodetection of the large form of the gamma 2 subunit of mammalian GABAA receptor.
Two forms of the GABAA receptor gamma 2-subunit, named short (gamma 2S) and long (gamma 2L), and generated by alternative RNA splicing, have been identified in mammalian brain by molecular cloning techniques. We have produced antibodies against a synthetic peptide containing the 8-amino acid insertion present in the long form but not in the short one. Using the antipeptide serum, we have identified the gamma 2L subunit in membrane preparations of GABAA receptors from rat and mouse cerebellum and its cellular location in cerebellum.
San Juan
I
I
Departamento de Bioquímica y Biología Molecular, Universidad de León, Spain.
Alonso
J M
JM
Ovalle
S
S
Negro
A
A
Chinchetru
M A
MA
Calvo
P
P
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D000917:Antibody Formation; D000918:Antibody Specificity; D001483:Base Sequence; D002531:Cerebellum / Q000737:chemistry*; D003001:Cloning, Molecular; D015151:Immunoblotting; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000032:analysis*; D051381:Rats; D011963:Receptors, GABA-A / Q000032:analysis*
D010446:Peptide Fragments; D011963:Receptors, GABA-A