title: Resetting the Drosophila clock by photic regulation of PER and a PER-TIM complex.
abstract: Circadian clocks can be reset by light stimulation. To investigate the mechanism of this phase shifting, the effects of light pulses on the protein and messenger RNA products of the Drosophila clock gene period (per) were measured. Photic stimuli perturbed the timing of the PER protein and messenger RNA cycles in a manner consistent with the direction and magnitude of the phase shift. In addition, the recently identified clock protein TIM (for timeless) interacted with PER in vivo, and this association was rapidly decreased by light. This disruption of the PER-TIM complex in the cytoplasm was accompanied by a delay in PER phosphorylation and nuclear entry and disruption in the nucleus by an advance in PER phosphorylation and disappearance. These results suggest a mechanism for how a unidirectional environmental signal elicits a bidirectional clock response.
authors.affiliation: Graduate Program in Molecular Genetics and Microbiology, Center for Advanced Biotechnology and Medicine, Rutgers University, Piscataway, NJ 08854, USA.
authors.forename: C
authors.initials: C
authors.lastname: Lee
authors.forename: V
authors.initials: V
authors.lastname: Parikh
authors.forename: T
authors.initials: T
authors.lastname: Itsukaichi
authors.forename: K
authors.initials: K
authors.lastname: Bae
authors.forename: I
authors.initials: I
authors.lastname: Edery
mesh_terms: D000818:Animals; D001683:Biological Clocks / Q000235:genetics; D001921:Brain / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D002940:Circadian Rhythm / Q000235:genetics; D003593:Cytoplasm / Q000378:metabolism; D003624:Darkness; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D005786:Gene Expression Regulation; D017344:Genes, Insect; D008027:Light; D009474:Neurons / Q000378:metabolism; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D056950:Period Circadian Proteins; D010766:Phosphorylation; D017956:Photoreceptor Cells, Invertebrate / Q000378:metabolism; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D029721:Drosophila Proteins; D009687:Nuclear Proteins; C539040:PER protein, Drosophila; D056950:Period Circadian Proteins; D011506:Proteins; D012333:RNA, Messenger; C096445:tim protein, Drosophila