title: [Study of the polymerization of fibrin using monoclonal antibodies 2D-2A and their Fab-fragments].
abstract: It has been shown that monAb's 2d-2a and their Fab-fragments are specific and effective inhibitors of fibrinogen clotting. Only one IgG molecule of monAb's 2d-2a can bind with one of their epitopes situated around peptide bond B beta Arg14-Gly15 in dimer fibrinogen molecule reducing the rate of protofibril lateral association and clot turbidity with only one fibrinopeptide B splitting off per fibrinogen molecule by thrombin. But two molecules of Fab-fragments of monAb's 2d-2a join to both of their epitopes and inhibit fibrinogen clotting dramatically without clot formation and with no fibrinopeptide B splitting off. These data suggest that the site of fibrin protofibril lateral coalescence is localized in NH2-terminal part of fibrin (ogen) B beta-chain, i.e. central E-domain of fibrin molecule takes part in protofibril lateral association. The mutual space orientation of NH2-terminal regions of fibrinogen B beta-chains is discussed.
authors.forename: E V
authors.initials: EV
authors.lastname: Lugovskoĭ
authors.forename: V S
authors.initials: VS
authors.lastname: Chudnovets
authors.forename: E M
authors.initials: EM
authors.lastname: Makogonenko
authors.forename: S G
authors.initials: SG
authors.lastname: Derzskaia
authors.forename: G K
authors.initials: GK
authors.lastname: Gogolinskaia
authors.forename: I N
authors.initials: IN
authors.lastname: Kolesnikova
authors.forename: L I
authors.initials: LI
authors.lastname: Mikhalovskaia
authors.forename: S V
authors.initials: SV
authors.lastname: Komissarenko
mesh_terms: D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D001704:Biopolymers; D001777:Blood Coagulation / Q000276:immunology*; D000939:Epitopes; D005340:Fibrinogen / Q000276:immunology*; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000276:immunology*; D010455:Peptides / Q000737:chemistry; D013917:Thrombin / Q000378:metabolism*
chemical_list: D000911:Antibodies, Monoclonal; D001704:Biopolymers; D000939:Epitopes; D007140:Immunoglobulin Fab Fragments; D010455:Peptides; D005340:Fibrinogen; D013917:Thrombin