Neurite outgrowth is enhanced by conditioning factor(s) released from central ganglia of Aplysia californica.
Single neurons of Aplysia californica were cultured on control and conditioned medium-treated cover slips (CM, derived from central ganglia of Aplysia) and fed with L-15 medium plus 20% hemolymph. After 5 h 36.9 +/- 3.3% of all neurons plated on CM-treated cover slips sprouted, whereas only 22.9 +/- 2.7% sprouted in the control group. After 24 h the average neurite length in the presence of putative conditioning factor(s) was 621 +/- 42 microns versus 411 +/- 20 microns in control, but the number of primary processes was the same (4.3 +/- 0.5 versus 4.2 +/- 0.9).
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Wadsworth Center for Laboratories & Research, NYS Department of Health, Albany 12201, USA.
Carpenter
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D000818:Animals; D001048:Aplysia; D002478:Cells, Cultured; D017077:Culture Media, Conditioned; D017952:Ganglia, Invertebrate / Q000378:metabolism*; D006458:Hemolymph / Q000378:metabolism / Q000502:physiology; D009414:Nerve Growth Factors / Q000378:metabolism / Q000502:physiology*; D016501:Neurites / Q000502:physiology*
D017077:Culture Media, Conditioned; D009414:Nerve Growth Factors