Aflatoxin exposure and cytogenetic alterations in individuals from the Gambia, West Africa.
Aflatoxin-albumin adducts in peripheral blood provide a measure of exposure to aflatoxin over the previous 2-3 months. In the present study, the levels of these adducts were determined in a group of individuals from The Gambia, West Africa and were compared in a cross-sectional study to the levels of various cytogenetic alterations (chromosomal aberrations, micronuclei, sister chromatid exchanges) in the same individuals to test whether an increase in genetic damage is associated with an increased exposure in this population. Of 35 subjects tested for aflatoxin-albumin adducts, all but 3 were positive. There were no correlations between the adduct level and the number of cytogenetic abnormalities at the individual level. A comparison of the cytogenetic alterations was made between Gambian individuals and a group of 22 healthy people from Italy where aflatoxin exposure is expected to be low. The levels of structural chromosomal aberrations, sister chromatid exchanges and micronuclei were all higher in the former group. Overall, these data are indicative of a higher exposure to genotoxins in Gambian subjects, one of which are aflatoxins, but suggest that more specific genetic markers of aflatoxin exposure are required to further examine the link between aflatoxin exposure and genetic alterations.
Miele
M
M
Istituto Nazionale per la Ricerca sul Cancro, Genova, Italy.
Donato
F
F
Hall
A J
AJ
Whittle
H
H
Chapot
B
B
Bonatti
S
S
De Ferrari
M
M
Artuso
M
M
Gallerani
E
E
Abbondandolo
A
A
Montesano
R
R
Wild
C P
CP
D000293:Adolescent; D000328:Adult; D000348:Aflatoxins / Q000009:adverse effects* / Q000032:analysis; D000367:Age Factors; D000368:Aged; D000418:Albumins / Q000032:analysis; D000704:Analysis of Variance; D002869:Chromosome Aberrations; D004781:Environmental Exposure; D004784:Environmental Monitoring; D005260:Female; D005714:Gambia; D005838:Genotype; D006514:Hepatitis B Surface Antigens / Q000097:blood; D006801:Humans; D007527:Isoenzymes / Q000235:genetics; D008297:Male; D015162:Micronucleus Tests; D008875:Middle Aged; D009153:Mutagens / Q000009:adverse effects*; D012044:Regression Analysis; D012727:Sex Characteristics; D012854:Sister Chromatid Exchange
D000348:Aflatoxins; D000418:Albumins; D006514:Hepatitis B Surface Antigens; D007527:Isoenzymes; D009153:Mutagens; C098408:aflatoxin-albumin adduct