Characterization and localization of the rat, mouse and human testicular phosphatidylethanolamine binding protein.
A cytosolic 23kDa protein was initially purified from bovine brain and shown to bind phosphatidylethanolamine. Later, it was also characterized in rat and human brain, and it is now known to be widespread, having been found in numerous tissues in several species. Here, we report the high level of mRNA and phosphatidyl ethanolamine binding protein expression in rat testis and to a lesser extent in mouse testis. In human testis, although it was not detectable by Northern blot analysis, the mRNA was shown to be present when PCR amplification was performed. Immunohistochemical experiments revealed that the testicular phosphatidylethanolamine binding protein (tPBP) is principally expressed in the elongated spermatids of both rat and mouse testis. This finding, and the association of tPBP with cellular membranes, suggest its possible implication in membrane remodelling during spermatid maturation.
Seddiqi
N
N
Laboratoire des protéines, CNRS URA 1188, Université René Descartes, Paris, France.
Segretain
D
D
Bucquoy
S
S
Pineau
C
C
Jégou
B
B
Jollès
P
P
Schoentgen
F
F
D000595:Amino Acid Sequence; D000727:Androgen-Binding Protein; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D001921:Brain / Q000378:metabolism; D002144:Callithrix; D002352:Carrier Proteins / Q000032:analysis / Q000096:biosynthesis* / Q000737:chemistry*; D002417:Cattle; D002478:Cells, Cultured; D003600:Cytosol / Q000378:metabolism; D018076:DNA, Complementary; D015870:Gene Expression; D015723:Gene Library; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008817:Mice, Mutant Strains; D008969:Molecular Sequence Data; D008970:Molecular Weight; D049488:Phosphatidylethanolamine Binding Protein; D010714:Phosphatidylethanolamines / Q000378:metabolism*; D049294:Phospholipid Transfer Proteins; D016133:Polymerase Chain Reaction; D060789:Prostatein; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D060765:Secretoglobins; D017386:Sequence Homology, Amino Acid; D013091:Spermatogenesis; D013737:Testis / Q000166:cytology / Q000378:metabolism*; D014598:Uteroglobin; D060788:Mammaglobin B
D000727:Androgen-Binding Protein; D002352:Carrier Proteins; D018076:DNA, Complementary; D049488:Phosphatidylethanolamine Binding Protein; D010714:Phosphatidylethanolamines; D049294:Phospholipid Transfer Proteins; D060789:Prostatein; D012333:RNA, Messenger; D060765:Secretoglobins; D014598:Uteroglobin; C511482:PEBP1 protein, human; C511484:Scgb1d2 protein, rat; C511483:Scgb1d4 protein, rat; C556468:Scgb2a1 protein, rat; D060788:Mammaglobin B