Automated sample preparation for cholesterol determination in foods.
An automated sample preparation system has been developed for the determination of cholesterol in a wide range of matrices. Isolation of cholesterol is performed with a robotic arm coupled with a series of modular stations. Samples are introduced into the system which adds the appropriate reagents, carries out the saponification, pH adjustment, solid-phase extraction and drying steps. This system was evaluated using 15 different food matrices. The average recovery for NIST standards exceeded 97%. A solution of n-hexane-2-propanol was substituted for the traditional methanol-chloroform extraction. Manual pH adjustment was replaced with a buffer. Manual and automated methods were compared and no difference was observed at the 95% confidence level.
Johnson
J H
JH
Kraft General Foods, Glenview, IL, USA.
McIntyre
P
P
Zdunek
J
J
D001331:Automation; D002784:Cholesterol / Q000032:analysis* / Q000302:isolation & purification; D002849:Chromatography, Gas; D003611:Dairy Products / Q000032:analysis; D005504:Food Analysis / Q000379:methods* / Q000592:standards; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D008460:Meat / Q000032:analysis; D012371:Robotics; D012997:Solvents
D012997:Solvents; D002784:Cholesterol