Relationship between humoral immunoaugmenting properties of DHEAS and IgD-receptor expression in young and aged mice.
IgD-receptors are associated with augmented antibody production in vivo and are induced on CD4+ T cells by aggregated IgD in young but not in aged mice. In the current study orally or intraperitoneally administered DHEAS was found to enhance antibody production, as measured in a plaque-forming cell assay, and also to cause an increased expression of IgD-R on T cells in both young and aged mice. IgD-R+ T cells are enumerated by rosette cell formation with IgD-coated SRBC. Since, as shown previously, the immunoaugmenting effect of IgD-R+ T cells is blocked by injection of monomeric IgD, the effect of monomeric IgD was also examined in DHEAS-pretreated mice. The inhibitory effect obtained with monomeric IgD in these studies indicates that upregulation of IgD-R by DHEAS plays an important role in the immunoenhancing effect of this hormone. In addition, no significant effect of DHEAS was obtained on contact hypersensitivity to DNCB or on proliferative responses of T cells from young or aged mice. Aged but not young mice showed increases in the numbers of Ia+ epidermal Langerhans cells after DHEAS treatment.
Swenson
C D
CD
Department of Pathology, NYU Medical Center, New York 10016, USA.
Gottesman
S R
SR
Belsito
D V
DV
Samanich
K M
KM
Edington
J
J
Thorbecke
G J
GJ
D000276:Adjuvants, Immunologic; D000375:Aging; D000818:Animals; D000917:Antibody Formation; D003687:Dehydroepiandrosterone / Q000031:analogs & derivatives* / Q000494:pharmacology; D019314:Dehydroepiandrosterone Sulfate; D003877:Dermatitis, Contact; D000949:Histocompatibility Antigens Class II / Q000378:metabolism; D006801:Humans; D007072:Immunoglobulin D / Q000378:metabolism; D007801:Langerhans Cells / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D011961:Receptors, Fc / Q000378:metabolism*; D013601:T-Lymphocytes / Q000378:metabolism*
D000276:Adjuvants, Immunologic; D000949:Histocompatibility Antigens Class II; D007072:Immunoglobulin D; D011961:Receptors, Fc; C050855:immunoglobulin D receptor; D003687:Dehydroepiandrosterone; D019314:Dehydroepiandrosterone Sulfate