Identification of rat bone morphogenetic protein-3b (BMP-3b), a new member of BMP-3.
Rat bone morphogenetic protein-3 (BMP-3) cDNA and BMP-3b cDNA were isolated from rat femur cDNA library by the RT-PCR method using degenerate oligonucleotide primers corresponding to human BMP-3. The deduced amino acid sequence of rat BMP-3 in the mature region reveals 2 amino acid changes compared to that of human BMP-3 (98% identity). The deduced BMP-3b amino acid sequence shows 81% similarity with the mature region of rat BMP-3 and only 37% similarity with the propeptide region. By Northern blot analysis, rat BMP-3b mRNA was detected in costa, costicartilage, femur, calvaria, trachea, aorta and brain. Among these tissues, BMP-3b transcripts were predominantly expressed in cerebellum. BMP-3 mRNA was found in femur, calvaria, trachea, lung and ovary. Although BMP-3b and BMP-3 are very closely related to each other, their transcripts are distributed in different tissues except that both are found in bone. The distribution pattern of BMP-3b mRNA suggests that BMP-3b plays an important role in the central nervous system as well as in bone formation and remodeling.
Takao
M
M
National Cardiovascular Center Research Institute, Fujishirodai, Suita, Osaka, Japan.
Hino
J
J
Takeshita
N
N
Konno
Y
Y
Nishizawa
T
T
Matsuo
H
H
Kangawa
K
K
D000375:Aging / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D055398:Bone Morphogenetic Protein 3; D019485:Bone Morphogenetic Proteins; D001842:Bone and Bones / Q000378:metabolism*; D002531:Cerebellum / Q000196:embryology / Q000378:metabolism*; D017931:DNA Primers; D018076:DNA, Complementary; D005314:Embryonic and Fetal Development; D005269:Femur; D015870:Gene Expression; D015723:Gene Library; D055413:Growth Differentiation Factor 10; D006133:Growth Substances / Q000032:analysis* / Q000096:biosynthesis; D006801:Humans; D008969:Molecular Sequence Data; D009928:Organ Specificity; D016133:Polymerase Chain Reaction; D014176:Protein Biosynthesis; D011506:Proteins / Q000032:analysis*; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D051381:Rats; D017386:Sequence Homology, Amino Acid
C525757:BMP3 protein, human; C525759:Bmp3 protein, rat; D055398:Bone Morphogenetic Protein 3; D019485:Bone Morphogenetic Proteins; D017931:DNA Primers; D018076:DNA, Complementary; C525785:GDF10 protein, human; C525786:Gdf10 protein, rat; D055413:Growth Differentiation Factor 10; D006133:Growth Substances; D011506:Proteins; D012333:RNA, Messenger