[Use of a nylon membrane for purifying DNA fragments from agarose gels].
A new and effective technique was proposed for rapid 30-40 minisolation of pure double-stranded DNA fragments from agarose gels and their use for nick-translation, for restriction with cutting enzymes and ligation with vector DNA. The technique developed involved an electrophoretic transferring of a DNA fragment from the gel into a strip of nylon membrane with the pore size of 0.2 mm followed by elution with buffer containing 0.1% SDS. Yield of the DNA fragment with a length up to 8.0 Kbp was at least 50%. Advantages of the technique developed as compared with standard methods are discussed.
Kulikov
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D004247:DNA / Q000737:chemistry / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel; D008567:Membranes, Artificial; D009757:Nylons
D008567:Membranes, Artificial; D009757:Nylons; D004247:DNA