Quantitative polymerase chain reaction in diagnosing ocular toxoplasmosis.
OBJECTIVE
To determine if quantitative competitive polymerase chain reaction can be used to diagnose ocular toxoplasmosis with certainty.


METHODS
We examined the vitreous humor of a patient with recurrent ocular toxoplasmosis by using quantitative competitive polymerase chain reaction.


RESULTS
The number of Toxoplasma gondii in the vitreous humor was quantified by quantitative competitive polymerase chain reaction.


CONCLUSIONS
Quantitative competitive polymerase chain reaction was useful for diagnosing ocular toxoplasmosis.
Norose
K
K
Department of Ophthalmology, Shinshu University School of Medicine, Japan.
Tokushima
T
T
Yano
A
A
D000328:Adult; D000818:Animals; D000900:Anti-Bacterial Agents / Q000627:therapeutic use; D000913:Antibodies, Protozoan / Q000032:analysis; D016054:DNA, Protozoan / Q000032:analysis; D004587:Electrophoresis, Agar Gel; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods*; D012008:Recurrence; D015572:Spiramycin / Q000627:therapeutic use; D014122:Toxoplasma / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D014126:Toxoplasmosis, Ocular / Q000175:diagnosis* / Q000188:drug therapy / Q000469:parasitology; D014822:Vitreous Body / Q000469:parasitology
D000900:Anti-Bacterial Agents; D000913:Antibodies, Protozoan; D016054:DNA, Protozoan; D015572:Spiramycin