Effects of sodium saccharin and linoleic acid on mRNA levels of Her2/neu and p53 in a human breast epithelial cell line.
The effects of two food-related chemicals (sodium saccharin and linoleic acid) on the levels of Her2/neu and p53 mRNA in a non-cancerous human breast epithelial cell line (HBL-100) were tested in comparison with the effects of the known tumor promoter phorbol 12-myristate 13-acetate (TPA). Treatments were made both with and without prior treatment with two well-known tumor initiators, N-nitroso-N-methylurea (NMU) or 7,12-dimethylbenz[a]anthracene (DMBA). The effects in general were small, the greatest being increases of 46-67% in Her2/neu mRNA levels in response to treatments with TPA or sodium saccharin following NMU treatments. These results demonstrate that sodium saccharin following NMU treatments might be involved in transcriptional regulation of Her2/neu in HBL-100 cells and suggest that its effects may not be limited to urinary bladder.
Ogretmen
B
B
Department of Biological, Chemical, and Physical Sciences, Illinois Institute of Technology, Chicago, 60616, USA.
Ratajczak
H
H
Gendel
S M
SM
Stark
B C
BC
D015127:9,10-Dimethyl-1,2-benzanthracene / Q000494:pharmacology; D001940:Breast / Q000187:drug effects* / Q000378:metabolism*; D002273:Carcinogens / Q000494:pharmacology; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D004848:Epithelium / Q000187:drug effects / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects; D018734:Genes, erbB-2; D016158:Genes, p53; D006801:Humans; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000494:pharmacology*; D008770:Methylnitrosourea / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000096:biosynthesis* / Q000235:genetics; D012439:Saccharin / Q000494:pharmacology*; D013549:Sweetening Agents / Q000494:pharmacology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis* / Q000235:genetics
D015127:9,10-Dimethyl-1,2-benzanthracene; D002273:Carcinogens; D019787:Linoleic Acid; D008041:Linoleic Acids; D008770:Methylnitrosourea; D012333:RNA, Messenger; D018719:Erb-b2 Receptor Tyrosine Kinases; D012439:Saccharin; D013549:Sweetening Agents; D013755:Tetradecanoylphorbol Acetate; D016159:Tumor Suppressor Protein p53