A colorimetric assay for cytokinin oxidase.
A simple and rapid colorimetric assay for cytokinin oxidase is described. The assay is based on the formation of a Schiff base between the enzymatic reaction product 3-methyl-2-butenal and p-aminophenol. The assay is effective in the submicromolar concentration range and can be used in crude plant extracts as well as in more highly purified preparations.
Libreros-Minotta
C A
CA
Department of Biochemistry, University of Missouri-Columbia 65211, USA.
Tipton
P A
PA
D002457:Cell Extracts / Q000737:chemistry*; D003124:Colorimetry; D010088:Oxidoreductases / Q000032:analysis*; D012680:Sensitivity and Specificity; D013053:Spectrophotometry; D013056:Spectrophotometry, Ultraviolet; D013997:Time Factors; D014908:Triticum / Q000166:cytology / Q000201:enzymology*
D002457:Cell Extracts; D010088:Oxidoreductases; C019984:cytokinin oxidase