Aluminum, calcium, and iron in the spinal cord of patients with sporadic amyotrophic lateral sclerosis using laser microprobe mass spectroscopy: a preliminary study.
We measured aluminum (Al), calcium (Ca), and iron (Fe) levels in neuronal cytoplasm and nucleus, capillaries, and neuropil in samples of ventral cervical spinal cord from 5 patients with sporadic amyotrophic lateral sclerosis (ALS) and 5 age-matched controls using laser microprobe mass spectrometry (LMMS). The concentration of Al was not altered in any area in the ALS samples. In contrast, Fe and Ca were increased 1.5-2-fold in the nucleus and cytoplasm of ALS neurons but not in capillaries and neuropil. These findings do not support the hypothesis that Al is enriched in spinal cord of sporadic ALS as has been reported for Guamanian ALS/Parkinson's dementia. The elevations of Fe in spinal neurons are consistent with reports of increased Fe in bulk samples of ALS spinal cord. The presence of increased Fe within spinal neurons may be significant in the pathogenesis of motor neuron degeneration by catalyzing the generation of reactive oxygen species within specific cells.
Kasarskis
E J
EJ
Department of Neurology, University of Kentucky, Lexington 40536-0084, USA.
Tandon
L
L
Lovell
M A
MA
Ehmann
W D
WD
D000368:Aged; D000535:Aluminum / Q000032:analysis*; D000690:Amyotrophic Lateral Sclerosis / Q000378:metabolism*; D002118:Calcium / Q000032:analysis*; D002196:Capillaries / Q000737:chemistry; D005260:Female; D006801:Humans; D007501:Iron / Q000032:analysis*; D007834:Lasers; D008297:Male; D013058:Mass Spectrometry; D008875:Middle Aged; D009046:Motor Neurons / Q000737:chemistry; D013116:Spinal Cord / Q000737:chemistry* / Q000166:cytology
D000535:Aluminum; D007501:Iron; D002118:Calcium