Analysis of glucan synthesis by Streptococcus mutans.
Glucan synthesis by Streptococcus mutans GS-5 has been analyzed by examining the regulation of expression and structure-function relationships of the glucosyltransferases. Primer extension analysis of the gtf genes has identified the putative promoter sequences for these genes and indicated that the -10 sequences are similar to the Escherichia coli consensus sequence. Site-directed mutagenesis as well as deletion analysis of the enzymes have identified amino acid residues as well as functional domains which play important roles in glucan synthesis.
Kuramitsu
H K
HK
Department of Oral Biology, State University of New York, Buffalo, USA.
Smorawinska
M
M
Nakano
Y J
YJ
Shimamura
A
A
Lis
M
M
D018829:Adhesins, Bacterial / Q000096:biosynthesis; D001665:Binding Sites; D017931:DNA Primers; D005798:Genes, Bacterial; D005936:Glucans / Q000096:biosynthesis*; D005964:Glucosyltransferases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D006801:Humans; D015394:Molecular Structure; D009055:Mouth / Q000382:microbiology; D016297:Mutagenesis, Site-Directed; D013295:Streptococcus mutans / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity
D018829:Adhesins, Bacterial; D017931:DNA Primers; D005936:Glucans; D005964:Glucosyltransferases