title: Quantitative polymerase chain reaction in diagnosing ocular toxoplasmosis.
abstract: OBJECTIVE
  To determine if quantitative competitive polymerase chain reaction can be used to diagnose ocular toxoplasmosis with certainty.
  
  
  METHODS
  We examined the vitreous humor of a patient with recurrent ocular toxoplasmosis by using quantitative competitive polymerase chain reaction.
  
  
  RESULTS
  The number of Toxoplasma gondii in the vitreous humor was quantified by quantitative competitive polymerase chain reaction.
  
  
  CONCLUSIONS
  Quantitative competitive polymerase chain reaction was useful for diagnosing ocular toxoplasmosis.
authors.affiliation: Department of Ophthalmology, Shinshu University School of Medicine, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Norose
authors.forename: T
authors.initials: T
authors.lastname: Tokushima
authors.forename: A
authors.initials: A
authors.lastname: Yano
mesh_terms: D000328:Adult; D000818:Animals; D000900:Anti-Bacterial Agents / Q000627:therapeutic use; D000913:Antibodies, Protozoan / Q000032:analysis; D016054:DNA, Protozoan / Q000032:analysis; D004587:Electrophoresis, Agar Gel; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods*; D012008:Recurrence; D015572:Spiramycin / Q000627:therapeutic use; D014122:Toxoplasma / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D014126:Toxoplasmosis, Ocular / Q000175:diagnosis* / Q000188:drug therapy / Q000469:parasitology; D014822:Vitreous Body / Q000469:parasitology
chemical_list: D000900:Anti-Bacterial Agents; D000913:Antibodies, Protozoan; D016054:DNA, Protozoan; D015572:Spiramycin