Rejection of cartilage formed by transplanted allogeneic chondrocytes: evaluation with monoclonal antibodies.
Cellular infiltrates participating in rejection of cartilage formed by transplanted allogeneic rat epiphyseal chondrocytes were evaluated immunohistochemically using a panel of different monoclonal antibodies. One week after transplantation, the grafts were surrounded by numerous class II MHC+ (OX6+, OX17+), CD4+ (W3/25+), and W3/13+ cells as well as some ED1+ monocytes/macrophages. Only a few T (OX19+) and B (HIS14+) cells were present. The number of class II MHC+ cells and ED1+ monocytes/macrophages did not change significantly in the course of rejection whereas the number of CD4+ and W3/13+ cells gradually decreased. On the other hand, there was a significant increase in the number of CD8+ (OX8+) cells. CD8+ cells accumulated close to the transplants and some of them penetrated cartilage matrix suggesting that they might be involved in chondrocyte killing. After 3 months, cartilage was almost completely destroyed and the intensity of infiltrations was markedly decreased. Fibrous connective tissue predominated, however, some class II+ as well as few ED1+, CD4+ and CD8+ cells were still present adjacent to the cartilage remnants. At the time of transplantation, chondrocytes were endowed with RT1.D class II antigen (OX17+), but they did not react with OX6 mAb (monoclonal antibody) recognizing the RT1.B class II molecule. However, after 1 week, some chondrocytes reacted with OX6 mAb and the number of RT1.B positive chondrocytes increased in the course of cartilage rejection.
Romaniuk
A
A
Department of Histology and Embryology, Warsaw Medical School, Poland.
Malejczyk
J
J
Kubicka
U
U
Hyc
A
A
Olszewski
W L
WL
Moskalewski
S
S
D000818:Animals; D000911:Antibodies, Monoclonal; D002356:Cartilage / Q000166:cytology / Q000276:immunology / Q000637:transplantation*; D002465:Cell Movement / Q000276:immunology; D006084:Graft Rejection / Q000276:immunology* / Q000473:pathology; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D014184:Transplantation, Homologous
D000911:Antibodies, Monoclonal; D000949:Histocompatibility Antigens Class II