Immunocytochemical localization of prostaglandin E2 receptor subtypes in porcine ocular tissues. II. Nonuveal tissues.
The localization of PGE2 receptor subtypes (EP1, EP2 and EP3) in nonuveal tissues of porcine eye was investigated by immunocytochemical methods with polyclonal antibodies directed against conserved peptide sequences in the carboxyl terminal region of the receptor subtypes. EP1 was found only in corneal epithelium, while EP2 immunoreactivity was associated with corneal epithelium and endothelium and lens epithelium. EP3 receptor subtype was localized to all ocular tissues examined (except lens fiber cells), i.e. corneal epithelium and endothelium, lens epithelium, retinal photoreceptor and pigmented epithelium, Bipolar cell, horizontal cell, amacrine cell, ganglion cell and Muller cell.
Zhao
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C
Kresge Eye Institute, Department of Ophthalmology, Wayne State University School of Medicine, Detroit, Michigan, USA.
Shichi
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H
D000818:Animals; D003315:Cornea / Q000737:chemistry* / Q000648:ultrastructure; D004848:Epithelium / Q000737:chemistry / Q000648:ultrastructure; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D007908:Lens, Crystalline / Q000737:chemistry* / Q000648:ultrastructure; D016253:Microscopy, Immunoelectron; D010446:Peptide Fragments / Q000276:immunology; D010857:Pigment Epithelium of Eye / Q000737:chemistry / Q000648:ultrastructure; D018078:Receptors, Prostaglandin E / Q000032:analysis* / Q000145:classification / Q000276:immunology; D012160:Retina / Q000737:chemistry* / Q000648:ultrastructure; D013552:Swine
D010446:Peptide Fragments; D018078:Receptors, Prostaglandin E