title: Detection of centromeres in vinblastine- and radiation-induced micronuclei of human lymphocytes using FISH with an alpha satellite pancentromeric DNA probe.
abstract: Fluorescence in situ hybridisation (FISH) with a human alphoid satellite pancentromeric DNA probe was used to detect centromeres in micronuclei of human lymphocytes induced by gamma irradiation and by Vinblastine sulfate. In a cytokinesis-block micro-nucleus assay a dose-dependent increase of micronuclei was detected for both agents. 72-89% of vinblastine-induced micronuclei, but only 7-48% of radiation-induced micronuclei showed centromere-positive fluorescence signals. Vinblastine treatment frequencies of centromere-negative micronuclei did not increase compared to control values, nor did frequencies of centromere-positive micronuclei in irradiated lymphocytes. Since FISH with an alpha satellite DNA probe allows the direct detection of centromeric DNA sequences the spindle damaging or clastogenic effectiveness of a compound can be easily and reliably examined in a cytokinesis-block micronucleus assay in human lymphocytes.
authors.affiliation: Institute für Strahlenbiologie, GSF-Forschungszentrum für Umwelt und Gesundheit, Oberschleissheim, Germany.
authors.forename: R
authors.initials: R
authors.lastname: Huber
authors.forename: K
authors.initials: K
authors.lastname: Salassidis
authors.forename: U
authors.initials: U
authors.lastname: Kulka
authors.forename: H
authors.initials: H
authors.lastname: Braselmann
authors.forename: M
authors.initials: M
authors.lastname: Bauchinger
mesh_terms: D000328:Adult; D000782:Aneuploidy; D000972:Antineoplastic Agents, Phytogenic / Q000633:toxicity*; D002503:Centromere / Q000187:drug effects / Q000528:radiation effects; D015342:DNA Probes; D004305:Dose-Response Relationship, Drug; D004307:Dose-Response Relationship, Radiation; D016650:Fluorescein-5-isothiocyanate; D005720:Gamma Rays / Q000009:adverse effects; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008214:Lymphocytes / Q000187:drug effects / Q000528:radiation effects / Q000648:ultrastructure*; D008297:Male; D048629:Micronuclei, Chromosome-Defective / Q000187:drug effects / Q000528:radiation effects / Q000648:ultrastructure*; D008856:Microscopy, Fluorescence; D014747:Vinblastine / Q000633:toxicity*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D015342:DNA Probes; D014747:Vinblastine; D016650:Fluorescein-5-isothiocyanate