Resolution of kinase activities during the HeLa cell cycle: identification of kinases with cyclic activities.
An in situ gel kinase assay was used to resolve kinase activities during the HeLa cell cycle. Three kinase activities changed dramatically during the cell cycle, whereas a number of others remained relatively constant. The former included histone kinases with estimated molecular masses of 46, 87, and 39 kDa. The 46-kDa kinase activity was preferentially expressed at least 10-fold greater in mitotic cells. In contrast, the 87- and 39-kDa kinases were most active early in G1 but their activity declined progressively as cells approached mitosis. The 87- but not the 46- or 39-kDa kinases had autophosphorylation activity. The identities of the kinases are unknown but they may play important roles in regulation of the cell cycle.
Monteiro
M J
MJ
University of Maryland Biotechnology Institute, University of Maryland School of Medicine, Baltimore 21201, USA.
Mical
T I
TI
D002453:Cell Cycle / Q000502:physiology*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D006367:HeLa Cells; D006657:Histones / Q000378:metabolism; D006801:Humans; D008938:Mitosis; D008970:Molecular Weight; D010766:Phosphorylation; D006656:Protamine Kinase / Q000737:chemistry / Q000378:metabolism*
D006657:Histones; D006656:Protamine Kinase