UV-B- and oxidative stress-induced increase in nicotinamide and trigonelline and inhibition of defensive metabolism induction by poly(ADP-ribose)polymerase inhibitor in plant tissue.
Nicotinamide and trigonelline contents increased in Catharanthus roseus tissue culture after exposure to 2,2'-azobis(2-amidinopropane)dihydrochloride (AAPH) or vanadylsulfate and in Pisum sativum leaves after exposure to UV-B radiation. Vanadylsulfate increased phenylalanine ammonia-lyase (PAL) activity and the content of reduced and oxidized gluthathione in C. roseus tissue culture. The increases in PAL activity caused by 2 mM AAPH or 0.2mM vanadylsulfate were prevented by 0.1 mM 3-aminobenzamide (3-AB), an inhibitor of poly(ADP-ribose)polymerase. Present results support the hypothesis [Berglund, T., FEBS Lett. (1994) 351, 145-149] that nicotinamide and/or its metabolites may function as signal transmittors in the response to oxidative stress in plants and that poly(ADP-ribose)polymerase has a function in the induction of defensive metabolism.
Berglund
T
T
Department of Biochemistry and Biotechnology, Royal Institute of Technology, Stockholm, Sweden.
Kalbin
G
G
Strid
A
A
Rydström
J
J
Ohlsson
A B
AB
D000470:Alkaloids / Q000378:metabolism*; D000578:Amidines / Q000494:pharmacology; D001549:Benzamides / Q000494:pharmacology*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005978:Glutathione / Q000031:analogs & derivatives / Q000378:metabolism; D019803:Glutathione Disulfide; D009536:Niacinamide / Q000378:metabolism*; D018384:Oxidative Stress; D018532:Pisum sativum / Q000378:metabolism / Q000528:radiation effects; D010650:Phenylalanine Ammonia-Lyase / Q000378:metabolism; D010944:Plants / Q000187:drug effects / Q000378:metabolism*; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; D014466:Ultraviolet Rays; D017968:Vanadium Compounds / Q000494:pharmacology
D000470:Alkaloids; D000578:Amidines; D001549:Benzamides; D004791:Enzyme Inhibitors; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; D017968:Vanadium Compounds; D009536:Niacinamide; C009560:trigonelline; C034028:vanadyl sulfate; C046728:2,2'-azobis(2-amidinopropane); C025160:3-aminobenzamide; D010650:Phenylalanine Ammonia-Lyase; D005978:Glutathione; D019803:Glutathione Disulfide