Highly sensitive immunoradiometric assay for serum thyroglobulin with minimal interference from autoantibodies.
Five monoclonal antibodies (MAbs) directed against antigenic domains on thyroglobulin (Tg) not recognized by most anti-Tg human autoantibodies (aAbs) have been used to develop an improved IRMA for serum Tg with a limit of detection of 0.2 micrograms/L. Samples are incubated for 3 h in tubes coated with four anti-Tg MAbs. After washing, the tubes are incubated with the tracer MAb for 20 h at room temperature. Dilution and reproducibility tests demonstrated assay reliability. Tests performed on samples with (n = 361) or without (n = 283) aAbs showed that the TG IRMA Pasteur is largely independent of the marked interference generally caused by aAbs. These results were confirmed with an extended population of 2759 samples. For a cutoff of 1 micrograms/L, sensitivity and specificity were 0.97 and 1, respectively, in a follow-up of differentiated thyroid carcinoma in patients treated by total thyroidectomy.
Marquet
P Y
PY
ERIA Diagnostics Pasteur, Marnes la Coquette, France.
Daver
A
A
Sapin
R
R
Bridgi
B
B
Muratet
J P
JP
Hartmann
D J
DJ
Paolucci
F
F
Pau
B
B
D000818:Animals; D000911:Antibodies, Monoclonal; D001323:Autoantibodies / Q000097:blood*; D006801:Humans; D015592:Immunoradiometric Assay / Q000379:methods*; D051379:Mice; D009362:Neoplasm Metastasis; D012372:ROC Curve; D011933:Reagent Kits, Diagnostic; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013954:Thyroglobulin / Q000097:blood*; D013964:Thyroid Neoplasms / Q000097:blood / Q000532:radiotherapy / Q000601:surgery; D013965:Thyroidectomy
D000911:Antibodies, Monoclonal; D001323:Autoantibodies; D011933:Reagent Kits, Diagnostic; C006606:anti-thyroglobulin; D013954:Thyroglobulin