Sequence of the canine c-yes proto-oncogene.
Cloning of the canine yes oncogene was attempted from a cDNA library derived from a healthy canine spleen using a human c-yes-1 probe. The nucleotide and amino acid sequences revealed that the canine yes gene contained an open reading frame consisting of 539 amino acids. Its product had a molecular mass of 60,368 Daltons and showed 95.9 per cent and 90.4 per cent homology with human and chick p61c-yes, respectively. Moreover, the product had a myristylation signal, src homology region (SH) 3, SH2, and tyrosine kinase domains showing 98.8 per cent and 96.0 per cent homology with those of human beings and chickens, respectively. These findings indicate that the products of the canine yes gene may have non-receptor-type tyrosine kinase activity on the cell membrane, as is the case in human and chick p61c-yes.
Zhao
D M
DM
Department of Veterinary Pathology, Faculty of Agriculture, Miyazaki University, Japan.
Tateyama
S
S
Miyoshi
N
N
Uchida
K
K
Yamaguchi
R
R
Yamagami
T
T
Hayashi
T
T
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001483:Base Sequence; D002645:Chickens; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis / Q000737:chemistry* / Q000235:genetics; D004285:Dogs / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins / Q000737:chemistry* / Q000235:genetics*; D051575:Proto-Oncogene Proteins c-yes; D011519:Proto-Oncogenes / Q000235:genetics*; D015183:Restriction Mapping; D019061:src-Family Kinases
D000596:Amino Acids; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins; D004247:DNA; D051575:Proto-Oncogene Proteins c-yes; D019061:src-Family Kinases