title: In vitro association of mitochondrial ATP- dependent protease with mitochondrial heat-shock proteins.
abstract: Specific antibodies against the mitochondrial ATP- dependent protease and heat-shock proteins were used to study the association of these proteins with an abnormal bacterial protein, CRAG. It was shown that the mitochondrial ATP-dependent protease from rat liver and Zajdela hepatoma bind to the CRAG protein and that this binding was mediated through the heat-shock proteins. It was also demonstrated that the protease associated with heat-shock proteins is capable of degrading large proteins as well as small peptides in an ATP-dependent fashion. Zajdela hepatoma mitochondria, with enhanced mitochondrial proteolysis, were shown to contain more ATP-dependent protease associated with heat-shock proteins.
authors.affiliation: Cancer Research Institute, Slovak Academy of Sciences, Bratislava, Slovakia.
authors.forename: M
authors.initials: M
authors.lastname: Chloupková
authors.forename: K
authors.initials: K
authors.lastname: Luciaková
mesh_terms: D000255:Adenosine Triphosphate / Q000494:pharmacology*; D000818:Animals; D010450:Endopeptidases / Q000378:metabolism*; D006360:Heat-Shock Proteins / Q000378:metabolism*; D008297:Male; D008928:Mitochondria / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D006360:Heat-Shock Proteins; D000255:Adenosine Triphosphate; D010450:Endopeptidases