Identification of the cleavage site recognized by the turnip yellow mosaic virus protease.
The noncapsid protein expressed from ORF-206 of turnip yellow mosaic virus (TYMV) is autocatalytically processed by a papain-like protease, producing N-terminal 150-kDa and C-terminal 70-kDa proteins. By introducing two methionine residues near the N-terminus of the 70-kDa protein, we have obtained N-terminal amino acid sequence of that protein produced from [35S]methionine-labeled in vitro translations. The introduction of methionine residues was demonstrated to not interfere with viral replication or proteolysis, as assayed by inoculating mutant RNA transcripts onto whole plants and protoplasts, as well as by translating the RNAs in a rabbit reticulocyte lysate. This has allowed us to determine that the TYMV protease cleaves between alanine1259 and threonine1260 of the precursor protein p206, yielding proteins of calculated Mr 140,618 and 66,037, which will be referred to henceforth as p141 and p66, respectively. The sequence context around the cleavage site is LNGA/TP.
Bransom
K L
KL
Department of Agricultural Chemistry, Oregon State University, Corvallis 97331-7301, USA.
Wallace
S E
SE
Dreher
T W
TW
D000595:Amino Acid Sequence; D001483:Base Sequence; D001665:Binding Sites; D010450:Endopeptidases / Q000378:metabolism*; D008969:Molecular Sequence Data; D016296:Mutagenesis; D013379:Substrate Specificity; D017806:Tymovirus / Q000201:enzymology*; D017361:Viral Nonstructural Proteins / Q000235:genetics / Q000378:metabolism*
C088367:150 kDa protein, turnip yellow mosaic virus; C088365:70 kDa protein, turnip yellow mosaic virus; D017361:Viral Nonstructural Proteins; D010450:Endopeptidases