Purification of His6-tagged Rab1 proteins using bacterial and insect cell expression systems.
Nuoffer
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Department of Cell Biology, Scripps Research Institute, La Jolla, California 92037, USA.
Peter
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Balch
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D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D002846:Chromatography, Affinity / Q000379:methods; D002850:Chromatography, Gel / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000379:methods; D003001:Cloning, Molecular / Q000379:methods; D004926:Escherichia coli; D020558:GTP Phosphohydrolases / Q000096:biosynthesis / Q000302:isolation & purification*; D019204:GTP-Binding Proteins / Q000096:biosynthesis / Q000302:isolation & purification*; D015870:Gene Expression; D005822:Genetic Vectors; D006639:Histidine; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000302:isolation & purification; D018411:Spodoptera; D014162:Transfection / Q000379:methods; D020761:rab1 GTP-Binding Proteins
D011994:Recombinant Proteins; D006639:Histidine; D020558:GTP Phosphohydrolases; D019204:GTP-Binding Proteins; C061569:RAB1B protein, rat; D020761:rab1 GTP-Binding Proteins