title: Rhamnogalacturonase B from Aspergillus aculeatus is a rhamnogalacturonan alpha-L-rhamnopyranosyl-(1-->4)-alpha-D-galactopyranosyluronide lyase.
abstract: The recently described rhamnogalacturonase B, which is able to degrade ramified hairy regions of pectin, was found to be a rhamnogalacturonan alpha-L-rhamnopyranosyl-(1-->4)-alpha-D-galactopyranosyluronide lyase. The cleavage site and mechanism differ from that of the previously described rhamnogalacturonase A, which is a hydrolase and can now be termed rhamnogalacturonan alpha-D-galactopyranosyluronide-(1-->2)-alpha-L-rhamnopyranosyl hydrolase.
authors.affiliation: Wageningen Agricultural University, Department of Food Chemistry, The Netherlands.
authors.forename: M
authors.initials: M
authors.lastname: Mutter
authors.forename: I J
authors.initials: IJ
authors.lastname: Colquhoun
authors.forename: H A
authors.initials: HA
authors.lastname: Schols
authors.forename: G
authors.initials: G
authors.lastname: Beldman
authors.forename: A G
authors.initials: AG
authors.lastname: Voragen
mesh_terms: D001230:Aspergillus / Q000201:enzymology*; D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D004798:Enzymes / Q000145:classification; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000378:metabolism; D011133:Polysaccharide-Lyases / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D013379:Substrate Specificity
chemical_list: D004798:Enzymes; D009844:Oligosaccharides; D011994:Recombinant Proteins; D011133:Polysaccharide-Lyases; C098240:rhamnogalacturonase B