title: A high-performance liquid chromatographic assay for the determination of itraconazole concentration using solid-phase extraction and small sample volume.
abstract: Itraconazole has been shown to be highly effective against a broad spectrum of fungi, particularly aspergillus. In order to achieve the desired pharmacological effect, the itraconazole plasma concentration should be > 250 ng/ml. We developed a high-performance liquid chromatography assay for the rapid determination of plasma itraconazole concentration using solid-phase extraction and small sample volume. The assay possessed linearity up to 5,000 ng/ml, sensitivity of 50 ng/ml, average recovery of 100.8%, and run-to-run precision (n = 10) for concentrations of 100, 200, and 500 ng/ml of 12, 6.6, and 5.8%, respectively. Furthermore, the assay proved to be free of interference from 48 commonly prescribed and some over-the-counter medications. We conclude that the method described here is ideally suited for the therapeutic monitoring of itraconazole.
authors.affiliation: Department of Laboratory Medicine, Children's Hospital, Boston, Massachusetts 02115, USA.
authors.forename: N
authors.initials: N
authors.lastname: Rifai
authors.forename: M
authors.initials: M
authors.lastname: Sakamoto
authors.forename: O
authors.initials: O
authors.lastname: Platt
authors.forename: C
authors.initials: C
authors.lastname: Brugnara
mesh_terms: D000097:Acetonitriles / Q000737:chemistry; D000935:Antifungal Agents / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004347:Drug Interactions; D016903:Drug Monitoring; D006801:Humans; D017964:Itraconazole / Q000097:blood*; D012044:Regression Analysis
chemical_list: D000097:Acetonitriles; D000935:Antifungal Agents; D017964:Itraconazole; C032159:acetonitrile