title: Relative resistance to HIV-1 infection of CD4 lymphocytes from persons who remain uninfected despite multiple high-risk sexual exposure.
abstract: Some individuals remain uninfected with human immunodeficiency virus type-1 (HIV-1) despite multiple high-risk sexual exposures. We studied a cohort of 25 subjects with histories of multiple high-risk sexual exposures to HIV-1 and found that their CD8+ lymphocytes had greater anti-HIV-1 activity than did CD8+ lymphocytes from nonexposed controls. Further studies indicated that their purified CD4+ lymphocytes were less susceptible to infection with multiple primary isolates of HIV-1 than were CD4+ lymphocytes from the nonexposed controls. This relative resistance to HIV-1 infection did not extend to T-cell line-adapted strains, was restricted by the envelope glycoprotein, was not explained by the cell surface density of CD4 molecules, but was associated with the activity of the C-C chemokines RANTES, MIP-1alpha, and MIP-1beta. This relative resistance of CD4+ lymphocytes may contribute to protection from HIV-1 in multiply exposed persons.
authors.affiliation: Aaron Diamond AIDS Research Center, New York, New York 10016, USA.
authors.forename: W A
authors.initials: WA
authors.lastname: Paxton
authors.forename: S R
authors.initials: SR
authors.lastname: Martin
authors.forename: D
authors.initials: D
authors.lastname: Tse
authors.forename: T R
authors.initials: TR
authors.lastname: O'Brien
authors.forename: J
authors.initials: J
authors.lastname: Skurnick
authors.forename: N L
authors.initials: NL
authors.lastname: VanDevanter
authors.forename: N
authors.initials: N
authors.lastname: Padian
authors.forename: J F
authors.initials: JF
authors.lastname: Braun
authors.forename: D P
authors.initials: DP
authors.lastname: Kotler
authors.forename: S M
authors.initials: SM
authors.lastname: Wolinsky
authors.forename: R A
authors.initials: RA
authors.lastname: Koup
mesh_terms: D015496:CD4-Positive T-Lymphocytes / Q000821:virology*; D018414:CD8-Positive T-Lymphocytes / Q000821:virology*; D002478:Cells, Cultured; D018925:Chemokines / Q000276:immunology; D004198:Disease Susceptibility; D015658:HIV Infections / Q000276:immunology* / Q000821:virology; D018023:HIV Seronegativity / Q000276:immunology*; D015497:HIV-1 / Q000502:physiology*; D006801:Humans; D012309:Risk-Taking; D012725:Sexual Behavior; D014779:Virus Replication
chemical_list: D018925:Chemokines