[A method for determining the oxidase activity of ceruloplasmin after electrophoresis with sodium dodecylsulfate].
A method to determine ceruloplasmin oxidase activity after electrophoretic fractionation in polyacrylamide gel in presence of an anionic detergent sodium dodecylsulfate (SDS) has been developed. It was shown that during fractionation of blood plasma proteins by electrophoresis with SDS system electrophoretic peptides mobility does not depend upon the presence of mercaptoethanol. The absorption maximum on spectrophotometer while measuring the oxidase activity of ceruloplasmin after Ravin in the presence of DS-Na has been shifted from 530 nm to 550 nm.
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D002570:Ceruloplasmin / Q000378:metabolism*; D005591:Chemical Fractionation; D004591:Electrophoresis, Polyacrylamide Gel; D013053:Spectrophotometry
D002570:Ceruloplasmin