Activity of lipopolysaccharide-binding protein-bactericidal/permeability-increasing protein fusion peptide in an experimental model of Pseudomonas sepsis.
A chimeric protein consisting of the N-terminal domain of lipopolysaccharide-binding protein and the C-terminal domain of bactericidal/permeability-increasing protein demonstrated a dose-dependent survival benefit (P = 0.001) and reduced endotoxin levels (P < 0.01) in neutropenic rats with Pseudomonas aeruginosa sepsis. This lipopolysaccharide-binding protein-bactericidal/ permeability-increasing peptide has favorable pharmacokinetics and antiendotoxin properties which may be of value for human sepsis.
Opal
S M
SM
Infectious disease division, Memorial Hospital of Rhode Island, Pawtucket 02860-4499, USA.
Palardy
J E
JE
Jhung
J W
JW
Donsky
C
C
Romulo
R L
RL
Parejo
N
N
Marra
M N
MN
D000209:Acute-Phase Proteins; D000818:Animals; D023181:Antimicrobial Cationic Peptides; D001798:Blood Proteins / Q000493:pharmacokinetics / Q000627:therapeutic use*; D002352:Carrier Proteins / Q000493:pharmacokinetics / Q000627:therapeutic use*; D002462:Cell Membrane / Q000378:metabolism; D015169:Colony Count, Microbial; D004731:Endotoxins / Q000032:analysis; D005260:Female; D008033:Limulus Test; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D009503:Neutropenia / Q000150:complications; D011552:Pseudomonas Infections / Q000188:drug therapy* / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D011993:Recombinant Fusion Proteins / Q000493:pharmacokinetics / Q000627:therapeutic use; D018805:Sepsis / Q000188:drug therapy* / Q000378:metabolism / Q000382:microbiology; D000099231:Lipopolysaccharide-Binding Protein
D000209:Acute-Phase Proteins; D023181:Antimicrobial Cationic Peptides; D001798:Blood Proteins; D002352:Carrier Proteins; D004731:Endotoxins; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D011993:Recombinant Fusion Proteins; D000099231:Lipopolysaccharide-Binding Protein; C021623:bactericidal permeability increasing protein