Purification and characterization of cystatin from duck egg white.
It is the second peptidase inhibitor, after ovostatin, which showing the same antipapain activity in egg white in different avian species implies differences in amino-acid sequences. Cystatin from duck egg white was purified by carboxymethylpapain affinity chromatography and size-exclusion HPLC. The purified inhibitor which showed partial identity in the immunodiffusion test with chicken egg white cystatin, had an apparent molecular mass of 9.3 kDa as determined by SDS/PAGE. IEF analysis revealed five molecular forms of pI in the range 7.8-8.4. The obtained cystatin was neither glycosylated nor phosphorylated as it is in the case of chicken cystatin. The determined Ki (0.005 +/- 0.001 nM) was similar to that reported for human and chicken cystatin C.
Warwas
M
M
Department of Pharmaceutical Biochemistry, Medical Academy, Wrocław, Poland.
Gburek
J
J
Osada
J
J
Gołab
K
K
D000818:Animals; D002645:Chickens; D015891:Cystatins / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D015853:Cysteine Proteinase Inhibitors / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D004372:Ducks / Q000378:metabolism*; D005260:Female; D006031:Glycosylation; D006801:Humans; D007120:Immunochemistry; D007526:Isoelectric Point; D008970:Molecular Weight; D010063:Ovum / Q000737:chemistry*; D010206:Papain / Q000037:antagonists & inhibitors; D010766:Phosphorylation; D013045:Species Specificity
D015891:Cystatins; D015853:Cysteine Proteinase Inhibitors; D010206:Papain