Human sperm chromosome analysis after microinjection into hamster oocytes.
OBJECTIVE
Subzonal sperm insemination (SUZI) into hamster oocytes was performed to establish the karyotypes of the fertilizing spermatozoa.


METHODS
Spermatozoa from two males with normal semen parameters were microinjected. Of 72 (52 + 20) analyzed sperm chromosome metaphases, only 1 (1.4%) was considered abnormal, showing a structural abnormality.


RESULTS
No hyperhaploidy was observed. Rates of sperm chromosomal abnormalities after microinjection were not higher than those reported previously using zona-free egg insemination, suggesting that the SUZI procedure per se does not increase sperm chromosomal abnormalities.


CONCLUSIONS
The use of subzonal insemination into hamster oocytes for the study of human sperm chromosomes in males with low sperm counts is discussed.
Cozzi
J
J
Reproductive Biology Laboratory, Grenoble University Medical School, Albert Bonniot Institute, La Tronche, France.
Chevret
E
E
Rousseaux
S
S
Pelletier
R
R
Sèle
B
B
D000818:Animals; D002455:Cell Division / Q000502:physiology; D002869:Chromosome Aberrations; D025063:Chromosome Disorders; D002875:Chromosomes / Q000648:ultrastructure*; D006224:Cricetinae; D005260:Female; D006801:Humans; D007248:Infertility, Male / Q000209:etiology / Q000503:physiopathology; D007315:Insemination, Artificial / Q000379:methods / Q000592:standards; D007621:Karyotyping; D008297:Male; D008845:Microinjections; D009865:Oocytes / Q000502:physiology / Q000648:ultrastructure*; D013084:Sperm-Ovum Interactions / Q000502:physiology*; D013094:Spermatozoa / Q000502:physiology / Q000648:ultrastructure*