title: jun-B gene expression in rat retinal cells following focal retinal injury.
abstract: We examined jun-B gene expression in the rat retina after a focal retinal injury by in situ hybridization. 30 min after producing a penetrating retinal wound, the retina showed the expression of jun-B mRNA intensively in the inner nuclear layer (INL) and relatively sparsely in the ganglion cell layer. The double-labeled retina by immunocytochemistry and in situ hybridization demonstrated that jun-B mRNA within the INL surrounded S-100 protein immunoreactive cytoplasm in the middle sublayer of the INL. These results suggest that the jun-B expression is induced mainly in Müller cells following retinal damage.
authors.affiliation: Department of Ophthalmology, Hokkaido University School of Medicine, Sapporo, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Ohki
authors.forename: K
authors.initials: K
authors.lastname: Yoshida
authors.forename: A
authors.initials: A
authors.lastname: Yamakawa
authors.forename: T
authors.initials: T
authors.lastname: Harada
authors.forename: H
authors.initials: H
authors.lastname: Matsuda
authors.forename: J
authors.initials: J
authors.lastname: Imaki
mesh_terms: D000818:Animals; D003593:Cytoplasm / Q000378:metabolism; D015807:Eye Injuries, Penetrating / Q000378:metabolism*; D005786:Gene Expression Regulation; D016758:Genes, jun / Q000235:genetics; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D016755:Proto-Oncogene Proteins c-jun / Q000096:biosynthesis* / Q000235:genetics*; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D012160:Retina / Q000166:cytology / Q000293:injuries* / Q000378:metabolism*; D009418:S100 Proteins / Q000378:metabolism
chemical_list: D016755:Proto-Oncogene Proteins c-jun; D012333:RNA, Messenger; D009418:S100 Proteins