title: The protective effect of probucol on adriamycin nephrosis in the rat.
abstract: Recent research has indicated the role of reactive oxygen species (ROS) in experimental nephritis. We examined the role of ROS and the effect of probucol, an anti-hyperlipidemic drug with antioxidant activity, on adriamycin (ADR)-induced nephrosis in the rat. Fourteen days after single intravenous injection of ADR (7.5 mg/kg b.w.), a nephrotic state was observed. Compared with the normal control values, the total kidney glutathione content was lower on day 5, but significantly higher on day 14 in the ADR-injected rats. Feeding ADR-injected rats with food containing 1% probucol was effective in reducing urinary protein excretion. Serum lipid peroxide level and kidney total glutathione content, both of which increased on day 14 in the ADR-injected rats, were also decreased significantly by concomitant probucol treatment. During long-term observation period of 18 weeks, probucol treatment relieved both urinary protein excretion and the progression of renal impairment. These protective effects of probucol suggest a role of ROS in the induction and progression of ADR nephrosis.
authors.affiliation: First Department of Internal Medicine, Aichi Medical University, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Futenma
authors.forename: H
authors.initials: H
authors.lastname: Miyai
authors.forename: T
authors.initials: T
authors.lastname: Iida
authors.forename: T
authors.initials: T
authors.lastname: Hara
authors.forename: T
authors.initials: T
authors.lastname: Watanabe
authors.forename: S
authors.initials: S
authors.lastname: Shiono
authors.forename: A
authors.initials: A
authors.lastname: Fukatsu
authors.forename: K
authors.initials: K
authors.lastname: Kato
mesh_terms: D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000009:adverse effects*; D000924:Anticholesteremic Agents / Q000627:therapeutic use*; D000975:Antioxidants / Q000627:therapeutic use*; D004317:Doxorubicin / Q000009:adverse effects*; D008297:Male; D009393:Nephritis / Q000139:chemically induced* / Q000517:prevention & control*; D011341:Probucol / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species
chemical_list: D000903:Antibiotics, Antineoplastic; D000924:Anticholesteremic Agents; D000975:Antioxidants; D017382:Reactive Oxygen Species; D004317:Doxorubicin; D011341:Probucol