title: Purification and properties of the xylanases from the termite Macrotermes bellicosus and its symbiotic fungus Termitomyces sp.
abstract: Four xylanases were purified, two from the termite Macrotermes bellicosus workers (XIT and X2T) and two from its symbiotic fungus Termitomyces sp. (X1Mc and X2Mc). The analysis of the step required for the purification of X1T and X1Mc and the comparison of their different properties suggested that xylanases X1T and X1Mc were the same enzyme, X1. The determination of the reducing sugars by TLC revealed that X1 was an endoxylanase (EC 3.2.1.8) and X2T and X2Mc were endoxylanases (EC 3.2.1.37). The apparent molecular weights of the three xylanases, determined by SDS-polyacrylamide gel electrophoresis, were 36 kDa for X1, 56 kDa for X2T and 22.5 kDa for X2Mc. The optimal pH of the three xylanases was approximately 5.5, and Km values determined with birchwood xylan as substrate were 0.2% for X1, 0.1% for X2T and 0.3% for X2Mc, showing a high affinity for this substrate. The three enzymes differed also by their thermal stability.
authors.affiliation: Laboratoire d'Ecophysiologie des Invertébrés, Université Paris XII-Val de Marne, Créteil, France.
authors.forename: M
authors.initials: M
authors.lastname: Matoub
authors.forename: C
authors.initials: C
authors.lastname: Rouland
mesh_terms: D000818:Animals; D001487:Basidiomycota / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007313:Insecta / Q000201:enzymology*; D007527:Isoenzymes / Q000737:chemistry / Q000302:isolation & purification*; D008970:Molecular Weight; D011489:Protein Denaturation; D013559:Symbiosis / Q000502:physiology; D043325:Xylan Endo-1,3-beta-Xylosidase; D014995:Xylosidases / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D007527:Isoenzymes; D014995:Xylosidases; D043325:Xylan Endo-1,3-beta-Xylosidase