title: Nucleotide sequence of 5'-upstream region and expression of a silkworm gene encoding a new member of the attacin family.
abstract: A genomic clone encoding a new member of attacin, an insect antibacterial protein, was isolated from a genomic library of the silkworm, Bombyx mori, and the nucleotide sequence of the 5'-upstream region was determined. The region contained Bm 1, a highly repetitive element of B. mori and a lipopolysaccharide (LPS) response element (RE)(NF-kappaB binding site), CAAT box and TATA box. Northern blot analysis showed that the attacin gene expression was rapidly induced by bacterial cell wall components such as LPS from Escherichia coli and peptidoglycan (PG) from Micrococcus luteus, suggesting that attacin plays an important role in an early phase of the self-defense system upon bacterial infection.
authors.affiliation: Laboratory of Biological Defense, National Institute of Sericultural and Entomological Science, Tsukuba, Ibaraki, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Taniai
authors.forename: T
authors.initials: T
authors.lastname: Ishii
authors.forename: M
authors.initials: M
authors.lastname: Sugiyama
authors.forename: A
authors.initials: A
authors.lastname: Miyanoshita
authors.forename: M
authors.initials: M
authors.lastname: Yamakawa
mesh_terms: D000818:Animals; D001483:Base Sequence; D012831:Bombyx / Q000235:genetics* / Q000378:metabolism*; D003001:Cloning, Molecular; D017931:DNA Primers; D018076:DNA, Complementary; D015870:Gene Expression; D017344:Genes, Insect; D015698:Genomic Library; D007301:Insect Hormones / Q000096:biosynthesis* / Q000235:genetics; D019476:Insect Proteins; D007313:Insecta; D008969:Molecular Sequence Data; D005810:Multigene Family; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D012689:Sequence Homology, Nucleic Acid; D016385:TATA Box
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D007301:Insect Hormones; D019476:Insect Proteins; C039711:attacin antibacterial protein, insect