title: Estimation of cholesterol sulphate in blood plasma and in erythrocyte membranes from individuals with Down's syndrome or diabetes mellitus type I.
abstract: OBJECTIVE
  Plasma and erythrocyte membrane cholesterol sulphate (CS) were measured in patients suffering from diabetes and Down's syndrome.
  
  
  METHODS
  The procedure for separation and determination of CS comprised HPTLC (high-performance thin-layer chromatography) and densitometry.
  
  
  RESULTS
  The mean plasma and RBC membranes CS concentrations (+/- SD) of the control group (n = 16) was 188 +/- 47 micrograms/dL and 343 +/- 57 micrograms/10(12) RBC, respectively. In 15 patients with diabetes and 12 Down's syndrome patients substantially higher CS levels were found (diabetes: plasma-348 +/- 60 micrograms/dL; RBC membranes-646 +/- 113 micrograms/10(12) RBC; Down's syndrome: plasma-245 +/- 54 micrograms/dL; RBC membranes 427 +/- 74 micrograms/10(12) RBC). Analysis of variance and multiple comparison (Newman-Keuls test) show statistically significant differences between all samples both for erythrocytes, F(2.41) = 52.24, p < 0.05, and plasma, F(2.41) = 34.92, p < 0.05.
  
  
  CONCLUSIONS
  It is postulated that differences in CS levels may contribute to changes of erythrocyte properties in these pathological states.
authors.affiliation: Department of Medical Biophysics, University of Lodz, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Przybylska
authors.forename: M
authors.initials: M
authors.lastname: Bryszewska
authors.forename: U
authors.initials: U
authors.lastname: Nowicka
authors.forename: K
authors.initials: K
authors.lastname: Szosland
authors.forename: J
authors.initials: J
authors.lastname: Kedziora
authors.forename: R M
authors.initials: RM
authors.lastname: Epand
mesh_terms: D000328:Adult; D002788:Cholesterol Esters / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002855:Chromatography, Thin Layer / Q000379:methods; D003922:Diabetes Mellitus, Type 1 / Q000097:blood*; D004314:Down Syndrome / Q000097:blood*; D004910:Erythrocyte Membrane / Q000737:chemistry*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D015233:Models, Statistical; D010949:Plasma / Q000737:chemistry*; D012984:Software
chemical_list: D002788:Cholesterol Esters; C007045:cholesteryl sulfate