title: Monoclonal antibodies against antigens exposed on the surface of vegetative forms and spores of Myxococcus virescens.
abstract: Twelve monoclonal antibodies (mAbs) directed against cell-surface antigens of Myxococcus virescens cells were developed and partially characterized. All of them recognized multiple, diffuse proteic bands in Western blot and four were also reactive to living bacteria, as assessed by flow cytometry. The four latter mAbs recognized antigens common to a number of vegetative forms and spores. The selective expression of proteins recognized by mAbs on the microorganisms and the possible applications of mAbs to the study of myxobacterial cell interaction are discussed.
authors.affiliation: Institute of Microbiology, University of Brescia, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Martinelli
authors.forename: R
authors.initials: R
authors.lastname: Pizzi
authors.forename: E
authors.initials: E
authors.lastname: Cabibbo
authors.forename: S
authors.initials: S
authors.lastname: Licenziati
authors.forename: F
authors.initials: F
authors.lastname: Dima
authors.forename: A D
authors.initials: AD
authors.lastname: Canaris
authors.forename: G
authors.initials: G
authors.lastname: Crea
authors.forename: G
authors.initials: G
authors.lastname: Ravizzola
authors.forename: A
authors.initials: A
authors.lastname: Caruso
authors.forename: A
authors.initials: A
authors.lastname: Turano
mesh_terms: D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D000942:Antigens, Bacterial; D000954:Antigens, Surface; D001426:Bacterial Proteins / Q000276:immunology; D016253:Microscopy, Immunoelectron; D016940:Myxococcus / Q000254:growth & development / Q000276:immunology* / Q000648:ultrastructure; D013171:Spores, Bacterial / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D000954:Antigens, Surface; D001426:Bacterial Proteins