Evaluation of the enzyme-linked immunosorbent assay for the rapid screening and detection of classical swine fever virus antigens in the blood of pigs.
A workshop was convened, at which seven enzyme-linked immunosorbent assays (ELISAs) were compared with virus isolation for the detection of viraemia in serial blood samples collected from six pigs at up to fourteen days after inoculation with classical swine fever virus. All ELISAs were of the double antibody sandwich type, using monoclonal and/or polyclonal antibodies to detect a variety of viral proteins in leukocytes, or in anti-coagulated blood or serum. Compared to virus isolation, specificity of the ELISA was good: only one sample found negative by virus isolation yielded a positive result in a single ELISA. Some false-negative results occurred with samples collected at up to eight days after inoculation, but all tests found samples collected between nine and fourteen days post-inoculation to be positive. The ELISAs require less-specialised facilities and can be performed much more rapidly than virus isolation. They are therefore extremely promising tools for screening large numbers of live pigs.
Depner
K
K
Community Reference Laboratory for Classical Swine Fever, Hanover Veterinary School, Germany.
Paton
D J
DJ
Central Veterinary Laboratory (Weybridge), Woodham Lane, Addlestone, Surrey KT15 3NB, United Kingdom
Crucière
C
C
Centre national d'études vétérinaires et alimentaires, Laboratoire central de recherches vétérinaires, 22, rue Pierre Curie, BP 67, 94703 Maisons-Alfort Cedex, France.
De Mia
G M
GM
Istituto Zooprofilattico Sperimental e dell'Umbria e delle Marche, Via G. Salvemini 1, 06100 Perugia, Italy.
Müller
A
A
Community Reference Laboratory for Classical Swine Fever, Hanover Veterinary School, Germany.
Koenen
F
F
Institut national de recherches vétérinaires, rue Groeselenberg 99,1180 Brussels, Belgium.
Stark
R
R
Bundesforschungsanstalt für Viruskrankheiten der Tiere, Paul-Ehrlich-Str. 28, Postfach 1149, 72001 Tübin gen, Germany. Present address: Institut für Virologie, Justus-Liebig-Universität Gießen, Frankfurter Straße 107, D-35392 Giessen, Germany
Liess
B
B
Community Reference Laboratory for Classical Swine Fever, Hanover Veterinary School, Germany.
D000357:African Swine Fever / Q000175:diagnosis*; D000358:African Swine Fever Virus / Q000276:immunology* / Q000302:isolation & purification; D000818:Animals; D000956:Antigens, Viral / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary*; D005069:Evaluation Studies as Topic; D005188:False Negative Reactions; D012680:Sensitivity and Specificity; D013552:Swine; D014766:Viremia / Q000175:diagnosis / Q000662:veterinary*
D000956:Antigens, Viral