title: Reperfusion hastens appearance and extent of distribution of type I collagen in infarct zone: immunohistochemical study in rat experimental infarction.
abstract: BACKGROUND
  The effects of reperfusion on time-dependent alteration of type I collagen have not been examined.
  
  
  OBJECTIVE
  We compared the sequential changes in the appearance and distribution of type I collagen in reperfused infarct rat hearts to those in non-reperfused hearts.
  
  
  METHODS
  Using an experimental rat model of infarction, we performed immunohistochemical staining with a polyclonal antibody to type I collagen by the avidin-biotin-peroxidase method. Reperfusion was established after 2-h coronary ligation that produced complete necrosis of myocytes.
  
  
  RESULTS
  In reperfused hearts, type I collagen appeared in the peripheral zone of the infarct at day 2, which was 1 day earlier than in non-reperfused hearts. The extent of distribution of type I collagen in reperfused hearts was comparable to that observed approximately 1 day later in non-reperfused hearts.
  
  
  CONCLUSIONS
  Reperfusion can accelerate collagen matrix formation compared with that in non-reperfused hearts after acute myocardial infarction.
authors.affiliation: First Department of Internal Medicine, Okayama University Medical School, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Yamasaki
authors.forename: S
authors.initials: S
authors.lastname: Kusachi
authors.forename: H
authors.initials: H
authors.lastname: Moritani
authors.forename: J
authors.initials: J
authors.lastname: Kondo
authors.forename: S
authors.initials: S
authors.lastname: Hirohata
authors.forename: A
authors.initials: A
authors.lastname: Tamura
authors.forename: T
authors.initials: T
authors.lastname: Tsuji
mesh_terms: D000818:Animals; D003094:Collagen / Q000032:analysis / Q000378:metabolism*; D005109:Extracellular Matrix / Q000378:metabolism; D007124:Immunoenzyme Techniques; D008297:Male; D009203:Myocardial Infarction / Q000378:metabolism*; D015425:Myocardial Reperfusion / Q000009:adverse effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
chemical_list: D003094:Collagen