Purification, characterization, crystallization, and preliminary X-ray results from Paracoccus denitrificans porin.
The porin from Paracoccus denitrificans ATCC 13543 was purified and crystallized. Two crystal forms were obtained from porin solutions with beta-d-octylglucopyranoside as detergent. Crystals of form I belong to the monoclinic spacegroup C2 with unit cell dimensions a = 112.2 A, b = 193.8 A, c = 100.5 A and beta = 129.2 degrees. There is 1 trimer per asymmetric unit. Crystals of form II are triclinic with a = 89.7 A, b = 98.8 A, c = 112.5 A, alpha = 112.5 degrees, beta = 101.8 degrees, gamma = 106.7 degrees (2 trimers per asymmetric unit). Both crystal forms diffract to 3 A.
Hirsch
A
A
Institut für Biophysik und Strahlenbiologie, Freiburg, Germany.
Wacker
T
T
Weckesser
J
J
Diederichs
K
K
Welte
W
W
D003460:Crystallization; D018360:Crystallography, X-Ray; D003902:Detergents; D005960:Glucosides; D010231:Paracoccus denitrificans / Q000737:chemistry*; D018272:Porins / Q000737:chemistry* / Q000302:isolation & purification; D011487:Protein Conformation
D003902:Detergents; D005960:Glucosides; D018272:Porins; C018619:octyl-beta-D-glucoside