Rearrangement-enhancing element upstream of the mouse immunoglobulin kappa chain J cluster.
Transcriptional regulatory elements have been shown to be necessary but not sufficient for the developmental regulation of immunoglobulin gene rearrangement in mouse precursor B cells. In the chicken lambda light chain locus, additional elements in the V-J intervening sequence are involved in negative and positive regulation of rearrangement. Here, mutation of the mouse homolog of a chicken element, located in the V(K)-J(K) intervening sequence upstream of the J(K) cluster, was shown to significantly decrease rearrangement. This cis-acting recombination-enhancing element affects the rearrangement process without being involved in regulating transcription.
Ferradini
L
L
INSERM U373, Institut Necker, Faculte de Medecine, Universite Paris V, France.
Gu
H
H
De Smet
A
A
Rajewsky
K
K
Reynaud
C A
CA
Weill
J C
JC
D000483:Alleles; D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology; D001483:Base Sequence; D002678:Chimera; D004742:Enhancer Elements, Genetic; D015322:Gene Rearrangement, B-Lymphocyte; D015329:Gene Rearrangement, T-Lymphocyte; D018390:Gene Targeting; D005803:Genes, Immunoglobulin; D007144:Immunoglobulin J-Chains / Q000235:genetics*; D007135:Immunoglobulin Variable Region / Q000235:genetics; D007145:Immunoglobulin kappa-Chains / Q000235:genetics*; D007438:Introns; D051379:Mice; D008810:Mice, Inbred C57BL; D016513:Mice, SCID; D008969:Molecular Sequence Data; D009154:Mutation; D011995:Recombination, Genetic; D012045:Regulatory Sequences, Nucleic Acid; D013234:Stem Cells
D007144:Immunoglobulin J-Chains; D007135:Immunoglobulin Variable Region; D007145:Immunoglobulin kappa-Chains