Increased expression of early growth response-1 messenger ribonucleic acid in prostatic adenocarcinoma.
OBJECTIVE
We isolated genes whose expression differs between normal and malignant prostate.


METHODS
Differential display polymerase chain reactions revealed a messenger ribonucleic acid (mRNA) with higher expression in prostatic adenocarcinoma versus age matched normal tissue. Deoxyribonucleic acid sequencing identified this mRNA as the product of the early growth response-1 gene.


RESULTS
Early growth response-1 mRNA levels were elevated in 12 of 12 intraprostatic adenocarcinomas but not in breast or ovarian cancers, or in rapidly dividing rat ventral prostate cells. Early growth response-1 mRNA was detected in epithelial and stromal cells at tumor margins but not in lymph node metastases.


CONCLUSIONS
Early growth response-1, a nuclear transcription factor, is implicated in the growth and invasion of intraprostatic cancers.
Thigpen
A E
AE
Department of Molecular Genetics, University of Texas Southwestern medical Center, Dallas, USA.
Cala
K M
KM
Guileyardo
J M
JM
Molberg
K H
KH
McConnell
J D
JD
Russell
D W
DW
D000230:Adenocarcinoma / Q000737:chemistry / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D004268:DNA-Binding Proteins / Q000235:genetics*; D051766:Early Growth Response Protein 1; D015972:Gene Expression Regulation, Neoplastic; D017874:Immediate-Early Proteins; D008297:Male; D008969:Molecular Sequence Data; D011471:Prostatic Neoplasms / Q000737:chemistry / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014157:Transcription Factors / Q000235:genetics*; D016335:Zinc Fingers / Q000235:genetics*
D004268:DNA-Binding Proteins; D051766:Early Growth Response Protein 1; C486110:Egr1 protein, rat; D017874:Immediate-Early Proteins; D012333:RNA, Messenger; D014157:Transcription Factors