title: Altered expression of carnitine palmitoyltransferase II in liver, muscle, and heart of mouse strain with juvenile visceral steatosis.
abstract: We conducted a quantitative study of the effect of carnitine deficiency on the mRNA level of carnitine palmitoyltransferase II in the liver, muscle and heart of mice with juvenile visceral steatosis, a strain that is systematically deficient in carnitine. The amount of carnitine palmitoyltransferase II mRNA was increased in liver and muscle of homozygotes, as compared with heterozygotes and normal controls, at 2, 4, and 8 wk of age. The mRNA levels of this enzyme were normalized after carnitine administration. The mRNA level of carnitine palmitoyltransferase II in the heart was increased only at 8 wk, and was not affected by carnitine administration. These results suggest that carnitine displays some effect on the mRNA level of the carnitine palmitoyltransferase II gene in liver and muscle, probably through fatty acid metabolic change.
authors.affiliation: Second Department of Internal Medicine, Osaka University Medical School, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Hotta
authors.forename: M
authors.initials: M
authors.lastname: Kuwajima
authors.forename: A
authors.initials: A
authors.lastname: Ono
authors.forename: R
authors.initials: R
authors.lastname: Uenaka
authors.forename: H
authors.initials: H
authors.lastname: Nakajima
authors.forename: J
authors.initials: J
authors.lastname: Miyagawa
authors.forename: M
authors.initials: M
authors.lastname: Namba
authors.forename: T
authors.initials: T
authors.lastname: Hanafusa
authors.forename: M
authors.initials: M
authors.lastname: Horiuchi
authors.forename: H
authors.initials: H
authors.lastname: Nikaido
authors.forename: J
authors.initials: J
authors.lastname: Hayakawa
authors.forename: N
authors.initials: N
authors.lastname: Kono
authors.forename: T
authors.initials: T
authors.lastname: Saheki
authors.forename: Y
authors.initials: Y
authors.lastname: Matsuzawa
mesh_terms: D000641:Ammonia / Q000097:blood; D000818:Animals; D002334:Carnitine O-Palmitoyltransferase / Q000235:genetics*; D005234:Fatty Liver / Q000201:enzymology*; D015971:Gene Expression Regulation, Enzymologic; D007003:Hypoglycemia / Q000201:enzymology*; D008099:Liver / Q000201:enzymology*; D051379:Mice; D008809:Mice, Inbred C3H; D009132:Muscles / Q000201:enzymology*; D009206:Myocardium / Q000201:enzymology*; D012333:RNA, Messenger / Q000032:analysis
chemical_list: D012333:RNA, Messenger; D000641:Ammonia; D002334:Carnitine O-Palmitoyltransferase