title: In vivo evidence that intercellular adhesion molecule-1 does not mediate endotoxin-induced hepatic leukocyte-endothelial cell interaction.
abstract: OBJECTIVE
  We have previously demonstrated that endotoxemia induces an immediate leukocytic response within the hepatic microvasculature, with leukostasis in sinusoids and leukocyte adherence in post-sinusoidal venules. We have now studied the role of intercellular adhesion molecule-1 in endotoxin-induced hepatic leukocyte-endothelial cell interaction in vivo using fluorescence microscopy.
  
  
  METHODS
  Sprague-Dawley rats were pretreated with either 1 mg/kg (n = 5) or 2 mg/kg (n = 4) of a monoclonal anti-rat anti-intercellular adhesion molecule-1 antibody intravenously, followed by exposure to endotoxin (E. coli LPS 10 mg/kg iv). Animals pretreated with an isotype-matched IgG1 control antibody (n = 5) served as controls. Intravital fluorescence microscopy for analysis of the hepatic microcirculation was performed prior to and 1 h after lipopolysaccharide exposure.
  
  
  RESULTS
  At 1 h after lipopolysaccharide-exposure, control animals showed a marked reduction of systemic leukocyte count, which was associated with a significant (p < 0.01) hepatic microvascular accumulation of leukocytes with stasis in sinusoids, as well as rolling and adherence in postsinusoidal venules. Pretreatment with anti-intercellular adhesion molecule-1 was not effective in preventing either systemic leukopenia or intravascular sequestration of leukocytes in endotoxemic livers.
  
  
  CONCLUSIONS
  Intercellular adhesion molecule-1 does not mediate endotoxin-induced early leukocytic response within the hepatic microcirculation.
authors.affiliation: Institute for Clinical and Experimental Surgery, University of Saarland, Homburg/Saar, Germany.
authors.forename: B
authors.initials: B
authors.lastname: Vollmar
authors.forename: A
authors.initials: A
authors.lastname: Senkel
authors.forename: M D
authors.initials: MD
authors.lastname: Menger
mesh_terms: D000818:Animals; D002450:Cell Communication / Q000187:drug effects; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects*; D018799:Intercellular Adhesion Molecule-1 / Q000502:physiology*; D007962:Leukocytes / Q000166:cytology / Q000187:drug effects*; D008070:Lipopolysaccharides; D008099:Liver / Q000166:cytology / Q000187:drug effects*; D008297:Male; D008856:Microscopy, Fluorescence; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D008070:Lipopolysaccharides; D018799:Intercellular Adhesion Molecule-1