[The effects of zhuling-duotang, hepatitis B vaccine and bacillus Calmette-Guerin on immunoactivities of peripheral blood mononuclear cells: an in vitro research].
"Zhuling-duotang (a polysaccharide preparation of the Chinese traditional herb medicine polyporusum bellatus) and hepatitis B vaccine (HB vaccine)" and "Persantin and bacillus calmeteguerin (BCG)" have been used to treat chronic hepatitis B. To elucidate their therapeutic mechanism, we studied the effects of zhuling-duotang, HB vaccine and BCG on immunoactivities of nomal human peripheral blood mononuclear cells (PBMCs). Cytotoxicity of PBMCs incubated with such drugs against target cells K562, HepG2 or 2.2.15 was detected by using 3H-TdR release assay. IL-2 and IFN-gamma activities of the supernatant were assayed. Immunofluorescent analysis of molecular CD4, CD8, CD16, IL-2R on the surface of PBMCs were done. The results showed that: (1) Zhuling-duotang and BCG could significantly increase the cytotoxicity of PBMCs. They could induce PBMCs to produce IL-2 but not IFN-gamma. They also could stimulate PBMCs to express IL-2R. Zhuling-duotang and BCG did not affect the percentage of CD4+, CD8+, CD16+ cells among PBMCs. (2) HB vaccine did not significantly increase the cytotoxicity of PBMCs. (3) The cytotoxicity of PBMCs induced by Zhuling-duotang combined with HB vaccine was not significantly higher than that of PBMCs induced by Zhuling-duotang alone.
Li
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L
302 Hospital of Chinese PLA, Beijing.
Wang
Q
Q
Si
C
C
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D001500:BCG Vaccine / Q000494:pharmacology*; D003602:Cytotoxicity, Immunologic / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D017325:Hepatitis B Vaccines / Q000494:pharmacology*; D006801:Humans; D007121:Immunocompetence; D007376:Interleukin-2 / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D015375:Receptors, Interleukin-2 / Q000096:biosynthesis
D000276:Adjuvants, Immunologic; D001500:BCG Vaccine; D004365:Drugs, Chinese Herbal; D017325:Hepatitis B Vaccines; D007376:Interleukin-2; D011134:Polysaccharides; D015375:Receptors, Interleukin-2