Altered production of endothelin-1 in the hypertrophied rat heart.
Although endothelin-1 (ET-1) has been shown to have potent hypertrophic effects in cultured cardiac myocytes, there is no evidence that the production of ET-1 is altered in the hypertrophied heart in vivo. We investigated mRNA and peptide levels of ET-1 in the rat heart hypertrophied due to pressure or volume overload. One week after surgery of abdominal aortic banding (AB) or aortic valve regurgitation (AR; the aortic valve was destroyed by a polyethylene catheter), the rats were sacrificed. Control sham-operated rats were also studied (AB-sham and AR-sham). The left ventricular (LV) weight to body weight ratio was significantly higher in AB rats than in AB-sham rats. It was also significantly higher in AR rats than in AR-sham rats. Therefore, AB rats and AR rats developed LV hypertrophy due to pressure and volume overload, respectively. The expression of ET-1 mRNA in the LV was markedly higher in AB rats than in AB-sham rats. The peptide level of ET-1 was also significantly higher in the LV of AB rats than in that of AB-sham rats. However, the expression of ET-1 mRNA in the LV of AR rats was similar to that of AR-sham rats. The present findings suggest that the production of ET-1 in the heart differs between pressure and volume overload-induced hypertrophy in rats.
Sakai
S
S
Department of Pharmacology, University of Tsukuba, Ibaraki, Japan.
Yorikane
R
R
Miyauchi
T
T
Sakurai
T
T
Kasuya
Y
Y
Yamaguchi
I
I
Sugishita
Y
Y
Goto
K
K
D000818:Animals; D006332:Cardiomegaly / Q000209:etiology / Q000378:metabolism*; D016232:Endothelins / Q000096:biosynthesis*; D006439:Hemodynamics; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D011519:Proto-Oncogenes; D051381:Rats; D017208:Rats, Wistar
D016232:Endothelins