title: Stimulatory effects of fibronectin and EGF on migration of corneal epithelial cells.
abstract: The effects of fibronectin and epidermal growth factor (EGF) on the migration of corneal epithelial cells in situ were investigated. When rabbit corneal blocks were cultured in serum-free medium with fibronectin or EGF, corneal epithelial cells migrated on the cut surface of the stroma. Histological and electron microscopic studies revealed that the leading edge of the fibronectin-treated cornea was thin, single-layered epithelium, whereas the leading edge of the EGF-treated cornea was a hypertrophic, multilayered epithelium. By autoradiography with 3H-thymidine, the authors observed that EGF stimulated corneal epithelial cell proliferation; however, fibronectin had no effect on cell proliferation. These results demonstrate that fibronectin and EGF enhance migration of corneal epithelial cells in different ways: Fibronectin facilitates sliding of epithelium, whereas EGF increases cell proliferation.
authors.affiliation: Department of Ophthalmology, Osaka University Medical School, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Watanabe
authors.forename: S
authors.initials: S
authors.lastname: Nakagawa
authors.forename: T
authors.initials: T
authors.lastname: Nishida
mesh_terms: D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D003315:Cornea / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D016895:Culture Media, Serum-Free; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D004847:Epithelial Cells; D004848:Epithelium / Q000187:drug effects / Q000502:physiology; D005353:Fibronectins / Q000302:isolation & purification / Q000494:pharmacology*; D008855:Microscopy, Electron, Scanning; D009924:Organ Culture Techniques; D011817:Rabbits
chemical_list: D016895:Culture Media, Serum-Free; D005353:Fibronectins; D004815:Epidermal Growth Factor