Alanyl-glutamine dipeptide-supplemented parenteral nutrition improves intestinal metabolism and prevents increased permeability in rats.
OBJECTIVE
The authors determined the effects of alanyl-glutamine-supplemented total parenteral nutrition (TPN) on mucosal metabolism, integrity, and permeability of the small intestine in rats.


METHODS
Male Sprague-Dawley rats were randomized to receive TPN supplemented with a conventional amino acids mixture (STD group) or the same solution supplemented with alanyl-glutamine; both solutions were isocaloric and isonitrogenous. On the seventh day of TPN, D-xylose and fluorescein isothiocyanate (FITC)-dextran were administered orally. One hour later, superior mesenteric vein (SMV) D-xylose and plasma FITC-dextran concentration were measured. Intestinal blood flow and calculated intestinal substrates flux were measured with ultrasonic transit time flowmetery.


RESULTS
Plasma FITC-dextran increased significantly in the STD group. Intestinal blood flow and SMV D-xylose concentration did not differ between the groups. Mucosa weight, villus height, mucosal wall thickness, mucosal protein, and DNA and RNA content in jejunal mucosa were significantly increased in the alanyl-glutamine group. Jejunal mucosal glutaminase activity and net intestinal uptake of glutamine (glutamine flux) were significantly higher in the alanyl-glutamine group as compared with the STD group.


CONCLUSIONS
Addition of alanyl-glutamine dipeptide to the TPN solution improves intestinal glutamine metabolism and prevents mucosal atrophy and deterioration of permeability.
Haque
S M
SM
Department of Pediatric Surgery, Osaka University Medical School, Japan.
Chen
K
K
Usui
N
N
Iiboshi
Y
Y
Okuyama
H
H
Masunari
A
A
Cui
L
L
Nezu
R
R
Takagi
Y
Y
Okada
A
A
D000818:Animals; D001783:Blood Flow Velocity; D002463:Cell Membrane Permeability / Q000187:drug effects; D004151:Dipeptides / Q000494:pharmacology / Q000627:therapeutic use*; D004353:Drug Evaluation, Preclinical; D005772:Gastrointestinal Transit / Q000187:drug effects; D007408:Intestinal Absorption / Q000187:drug effects; D007413:Intestinal Mucosa / Q000098:blood supply / Q000187:drug effects* / Q000378:metabolism*; D008297:Male; D010289:Parenteral Nutrition, Total / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley
D004151:Dipeptides; C054122:alanylglutamine