title: Is defibrotide's activity on leukocytes adenosine-receptor mediated? An "in vitro"--"ex vivo" appraisal.
abstract: Defibrotide both "in vitro" and "ex vivo" decreases PMN adhesion to nylon columns. This activity is blunted by 8-phenyltheophylline, a known blocker of adenosine A1/A2 receptors, suggesting that Defibrotide acts on that kind of receptors. Indomethacin, a blocker of PGI2 synthesis, did not cancel DFT's activity, suggesting that PGI2 is not involved in DFT's activity, at least under our experimental conditions. Non-PMN leukocytes behaved differently "ex vivo" than PMNs. DFT's activity was not blunted by 8-phenyltheophylline, suggesting that DFT acts on this kind of cell through a different mechanism. The "in vitro" experiment did not confirm "ex vivo" studies suggesting that in the "in vitro" experiment "something" is missing that is present in "ex vivo" experiments.
authors.affiliation: CRINOS Biological Research Laboratories, Villa Guardia (CO), Italy.
authors.forename: P
authors.initials: P
authors.lastname: Alberico
authors.forename: R
authors.initials: R
authors.lastname: Porta
authors.forename: R
authors.initials: R
authors.lastname: Pescador
authors.forename: L
authors.initials: L
authors.lastname: Ferro
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D005343:Fibrinolytic Agents / Q000494:pharmacology*; D007962:Leukocytes / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D011089:Polydeoxyribonucleotides / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D018047:Receptors, Purinergic P1 / Q000187:drug effects* / Q000378:metabolism; D013806:Theophylline / Q000031:analogs & derivatives / Q000494:pharmacology
chemical_list: D005343:Fibrinolytic Agents; D011089:Polydeoxyribonucleotides; D018047:Receptors, Purinergic P1; C036901:defibrotide; D013806:Theophylline; C028322:8-phenyltheophylline