Sigma s regulates pLS1 maintenance in stationary-phase Escherichia coli.
We have studied the influence of sigma s on the stability and number of copies of the promiscuous plasmid pLS1 in Escherichia coli. Our results indicate that pLS1 is less stable and has a lower number of copies in a rpoS mutant than in a wild-type strain during stationary phase. This behaviour does not seem to be due to differences in the expression of pLS1 replication regulators, but to be related to plasmid topology.
Espinosa-Urgel
M
M
Departamento de Bioquímica y Biología Molecular I, Facultad de Ciencias Biológicas, Universidad Complutense, Madrid, Spain.
Chamizo
C
C
Tormo
A
A
D001426:Bacterial Proteins / Q000502:physiology*; D004269:DNA, Bacterial / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development*; D010957:Plasmids / Q000502:physiology* / Q000648:ultrastructure; D011401:Promoter Regions, Genetic; D016196:S Phase; D012808:Sigma Factor / Q000502:physiology*
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D012808:Sigma Factor; C069567:sigma factor KatF protein, Bacteria