Quantitative analysis of pulmonary surfactant phospholipids by high-performance liquid chromatography and light-scattering detection.
An improved high-performance liquid chromatographic method for the separation and quantitation of nine phospholipid classes is described. It is based on normal-phase chromatography with silica gel as stationary phase and a binary gradient with mixtures of chloroform, methanol and water as mobile phase. The response of the evaporative light-scattering detector was non-linear. Peak areas were proportional to the power 1.7 of the masses. Phospholipids in lung lavage samples were enriched by liquid extraction prior to HPLC analysis. The described method is a rapid and accurate procedure for the quantitative analysis of phospholipid classes in biological samples.
Bünger
H
H
Klinik für Anaesthesiologie und Operative Intensivmedizin, Universitätsklinikum Rudolf Virchow, Freie Universität Berlin, Germany.
Pison
U
U
D000328:Adult; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007202:Indicators and Reagents; D008027:Light; D010713:Phosphatidylcholines / Q000032:analysis; D010743:Phospholipids / Q000032:analysis*; D011663:Pulmonary Surfactants / Q000032:analysis*; D012542:Scattering, Radiation; D013056:Spectrophotometry, Ultraviolet; D007507:Therapeutic Irrigation
D007202:Indicators and Reagents; D010713:Phosphatidylcholines; D010743:Phospholipids; D011663:Pulmonary Surfactants