Development and evaluation of a selective and indicative medium for isolation of Actinobacillus pleuropneumoniae from tonsils.
In order to isolate Actinobacillus pleuropneumoniae from mixed bacterial flora a selective and indicative medium was developed. The optimal concentrations of antibiotics were determined for selective chocolate agar (S-TSA) and selective blood agar (S-MBA) using a set of 25 strains of A. pleuropneumoniae. Following optimization of the media 96 isolates of A. pleuropneumoniae were tested on S-TSA and S-MBA. For isolation of A. pleuropneumoniae from 101 pig tonsils collected at slaughter the S-MBA proved significantly better than both S-TSA and the two non-selective agars tested. Furthermore, contaminating to S-TSA and the two non-selective media.
Jacobsen
M J
MJ
Danish Veterinary Laboratory, Copenhagen V, Denmark.
Nielsen
J P
JP
D000189:Actinobacillus Infections / Q000382:microbiology / Q000662:veterinary*; D016977:Actinobacillus pleuropneumoniae / Q000145:classification / Q000302:isolation & purification*; D000818:Animals; D003470:Culture Media; D006386:Hemagglutination Tests / Q000662:veterinary; D014066:Palatine Tonsil / Q000382:microbiology*; D012703:Serotyping / Q000662:veterinary; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*
D003470:Culture Media