Characterization of extrathymic T cells induced by recombinant murine interferon-beta in the peritoneal cavity of nude mice.
The effects of recombinant murine interferon-beta (rMuIFN-beta) on extrathymic lymphocyte formation in the peritoneal cavity of BALB/c athymic nude mice and tumor-bearing nude mice were examined for comparison with BALB/c normal euthymic mice. The 7 days of administration of 1 x 10(5) IU rMuIFN-beta caused the number of these cells to increase remarkably in the peritoneal cavity as a unique subset of asialoGM1+CD4+CD8-TcR alpha beta+ T cells. The asialoGM1+CD4+ T cells produced IL-2 and IFN-gamma in primary culture without stimulant but did not proliferate. Thus, extrathymic T cells can be induced easily in the peritoneal cavity in addition to the thymus for host defense systems.
Moriyama
M
M
Department of Allergology, Institute of Medical Science, Tokyo, Japan.
Harada
K
K
Kawasaki
H
H
Iwasaki
T
T
Yee
S T
ST
Nariuchi
H
H
Iigo
M
M
D000230:Adenocarcinoma / Q000276:immunology; D000818:Animals; D001483:Base Sequence; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D002478:Cells, Cultured; D003602:Cytotoxicity, Immunologic; D016130:Immunophenotyping; D007274:Injections, Intraperitoneal; D016899:Interferon-beta / Q000494:pharmacology*; D007376:Interleukin-2 / Q000378:metabolism; D051379:Mice; D008819:Mice, Nude; D008969:Molecular Sequence Data; D009368:Neoplasm Transplantation; D010529:Peritoneal Cavity / Q000166:cytology*; D011994:Recombinant Proteins / Q000494:pharmacology; D016176:T-Lymphocyte Subsets / Q000187:drug effects* / Q000648:ultrastructure
D007376:Interleukin-2; D011994:Recombinant Proteins; D016899:Interferon-beta