Gel chromatographic analysis of cyclosporin and its metabolites in human blood compartments.
Gel chromatography combined with specific and non-specific cyclosporin radioimmunoassays was adopted for quantitative analysis of cyclosporin and metabolites in free and protein-bound forms in blood compartments of kidney transplant patients. The analytical method was proved to be useful for the purpose, although plasma protein-bound forms of neither cyclosporin nor metabolites could be quantitated in the system. The present study also provided, by gel chromatographic analysis, additional examples to prove that concentrations of cyclosporin metabolites in blood compartments may not be deduced or inferred simply from those of cyclosporin.
Kodobayashi
M
M
Laboratory of Pharmaceutical Sciences, Osaka University School of Medicine, Japan.
Yamamoto
K
K
Takahara
S
S
Okuyama
A
A
Takashima
N
N
Sawada
M
M
Yanaihara
C
C
Kurokawa
N
N
D000284:Administration, Oral; D000328:Adult; D001798:Blood Proteins / Q000378:metabolism; D002850:Chromatography, Gel; D003524:Cyclosporins / Q000008:administration & dosage / Q000097:blood*; D006801:Humans; D016030:Kidney Transplantation; D008875:Middle Aged; D011485:Protein Binding; D011863:Radioimmunoassay
D001798:Blood Proteins; D003524:Cyclosporins