title: Anti-Pr cold agglutinins recognize immunodominant alpha 2,3- or alpha 2,6-sialyl groups on glycophorins.
abstract: Anti-Pr agglutinins (CAs) with the subspecificities anti-Pr1h, -Pr1d, -Pr2, -Pr3h, -Pr3d, -PrM and anti-Sa CAs recognize immunodominant N-acetylneuraminic acid (NeuN Ac) groups of tetra and/or trisaccharides (O-glycans) of glycophorin. These O-glycans are sialylated in alpha 2,3- and/or alpha 2,6-linkages. Sa and most Pr antigens have been inactivated by alpha 2,3-specific sialidases. Antigenicity was reconstituted on desialylated glycophorin by alpha 2,3-specific Gal beta 1,3GalN Ac-sialyltransferase indicating that alpha 2,3-linked NeuN Ac groups are the immunodominant components of Sa and most Pr antigens. Some Pr antigens were resistant to alpha 2,3-specific sialidase and were not reconstituted by alpha 2,3-specific Gal beta 1,3GalN Ac-sialyltransferase, which indicates that alpha 2,6-linked NeuN Ac group represents an immunodominant component of some Pr antigens.
authors.affiliation: Institute for Immunology, University of Heidelberg, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Kewitz
authors.forename: H J
authors.initials: HJ
authors.lastname: Gross
authors.forename: R
authors.initials: R
authors.lastname: Kosa
authors.forename: D
authors.initials: D
authors.lastname: Roelcke
mesh_terms: D000328:Adult; D000373:Agglutinins / Q000097:blood / Q000302:isolation & purification; D010367:Arachis; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D003080:Cold Temperature; D003450:Cryoglobulins; D004912:Erythrocytes / Q000276:immunology*; D006021:Glycophorins / Q000737:chemistry*; D006385:Hemagglutination Inhibition Tests; D006386:Hemagglutination Tests; D006388:Hemagglutinins; D006801:Humans; D007231:Infant, Newborn; D037102:Lectins; D008969:Molecular Sequence Data; D009439:Neuraminidase; D009844:Oligosaccharides / Q000737:chemistry*; D019887:Peanut Agglutinin; D037121:Plant Lectins; D012794:Sialic Acids / Q000032:analysis*; D012799:Sialyltransferases
chemical_list: D000373:Agglutinins; D003450:Cryoglobulins; D006021:Glycophorins; D006388:Hemagglutinins; D037102:Lectins; D009844:Oligosaccharides; D019887:Peanut Agglutinin; D037121:Plant Lectins; D012794:Sialic Acids; C029443:cold agglutinins; D012799:Sialyltransferases; D009439:Neuraminidase