Inactivation and unfolding of aminoacylase during denaturation in sodium dodecyl sulfate solutions.
During denaturation by sodium dodecyl sulfate (SDS), aminoacylase shows a rapid decrease in activity with increasing concentration of the detergent to reach complete inactivation at 1.0 mM SDS. The denatured minus native-enzyme difference spectrum showed two negative peaks at 287 and 295 nm. With the increase of concentration of SDS, both negative peaks increased in magnitude to reach maximal values at 5.0 mM SDS. The fluorescence emission intensity of the enzyme decreased, whereas there was no red shift of emission maximum in SDS solutions of increasing concentration. In the SDS concentration regions employed in the present study, no marked changes of secondary structure of the enzyme have been observed by following the changes in far-ultraviolet CD spectra. The inactivation of this enzyme has been followed and compared with the unfolding observed during denaturation in SDS solutions. A marked inactivation is already evident at low SDS concentration before significant conformational changes can be detected by ultraviolet absorbance and fluorescence changes. The inactivation rate constants of free enzyme and substrate-enzyme complex were determined by the kinetics method of the substrate reaction in the presence of inactivator previously described by Tsou [Tsou (1988), Adv. Enzymol. Related Areas Mol. Biol. 61, 381-436]. It was found that substrate protects against inactivation and at the same SDS concentrations, the inactivation rate of the free enzyme is much higher than the unfolding rate. The above results show that the active sites of metal enzyme containing Zn2+ are also situated in a limited and flexible region of the enzyme molecule that is more fragile to denaturants than the protein as a whole.
He
B
B
Department of Biological Sciences and Biotechnology, Tsing-hua University, Beijing, China.
Zhang
Y
Y
Zhang
T
T
Wang
H R
HR
Zhou
H M
HM
D000581:Amidohydrolases / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D001665:Binding Sites; D004789:Enzyme Activation / Q000187:drug effects; D007668:Kidney / Q000201:enzymology; D007700:Kinetics; D011487:Protein Conformation; D011489:Protein Denaturation; D017510:Protein Folding; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology*; D012996:Solutions; D013552:Swine; D015032:Zinc / Q000737:chemistry / Q000378:metabolism
D012996:Solutions; D012967:Sodium Dodecyl Sulfate; D000581:Amidohydrolases; C019672:aminoacylase I; D015032:Zinc