Identification of glycosaminoglycans using high-performance liquid chromatography on a hydroxyapatite column.
Glycosaminoglycans (heparin, heparan sulfate, dermatan sulfate, chondroitin sulfate, and hyaluronic acid) were labeled with a fluorescent reagent, 2-aminopyridine. The fluoro-labeled glycosaminoglycans were subjected to high-performance liquid chromatography on a hydroxyapatite column. The binding property of each glycosaminoglycan to hydroxyapatite was different. The structural properties of glycosaminoglycans bound to hydroxyapatite were then investigated using chemical desulfated or enzymic depolymerized glycosaminoglycans. This revealed that the sulfate content and molecular weight of the glycosaminoglycans correlated with their binding properties to hydroxyapatite. Desulfated dermatan sulfate but not desulfated chondroitin 6-sulfate bound to the hydroxyapatite. These data indicate that iduronic acid residues of glycosaminoglycans are important for the binding property. The method described which uses hydroxyapatite columns facilitates rapid separation and microanalysis of the glycosaminoglycans, especially dermatan sulfate and chondroitin sulfate.
Narita
H
H
Department of Biochemistry, Hirosaki University School of Medicine, Japan.
Takeda
Y
Y
Takagaki
K
K
Nakamura
T
T
Harata
S
S
Endo
M
M
D000631:Aminopyridines; D002809:Chondroitin Sulfates / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003871:Dermatan Sulfate / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D004187:Disaccharides / Q000737:chemistry; D017886:Durapatite; D005456:Fluorescent Dyes; D005965:Glucuronates / Q000737:chemistry; D020723:Glucuronic Acid; D006025:Glycosaminoglycans / Q000032:analysis* / Q000737:chemistry; D006497:Heparan Sulfate / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D007067:Iduronic Acid / Q000737:chemistry; D008970:Molecular Weight
D000631:Aminopyridines; D002809:Chondroitin Sulfates; D003871:Dermatan Sulfate; D004187:Disaccharides; D017886:Durapatite; D005456:Fluorescent Dyes; D005965:Glucuronates; D020723:Glucuronic Acid; D006025:Glycosaminoglycans; D006497:Heparan Sulfate; D007067:Iduronic Acid; C032439:alpha-aminopyridine