Primary structure of light and heavy chain variable regions of antibodies recognizing phosphorylated vimentins.
We determined the primary structure of three types of monoclonal antibodies against phosphorylated vimentin, 4A4, YT33, and MO82, which recognize phosphorylated Ser55, Ser33, and Ser82 on vimentin, respectively. The amino acid sequences between these antibodies and the anti-phosphotyrosine antibodies previously reported, (Asn/Gln)-X-(Gln/Tyr)-Ser-Tyr in the complimentarity determining region (CDR) 3 of the light chain of 4A4 and YT33, Asn-Pro-Asn-(Asn/Ser)-Gly-X-(Ser/Thr)-(Ser/Thr)-Tyr-Asn-Gln-(Arg/Lys)-Ph e-Lys in the heavy chain CDR2 of MO82, and Lys-X-Ser-(Ser/Asn) in the heavy chain CDR3 of YT33 and MO82, were highly conserved. These motifs may play a role in recognizing phosphate groups of phosphoserine and phosphotyrosine.
Tsujino
S
S
Department of Neurophysiology, Tokyo Metropolitan Institute of Gerontology, Itabashi-ku, Tokyo, Japan.
Sekimata
M
M
Inagaki
N
N
Kamie
Y
Y
Higashihara
M
M
Kurokawa
K
K
Imajoh-Ohmi
S
S
Inagaki
M
M
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000737:chemistry* / Q000276:immunology; D017931:DNA Primers; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D000939:Epitopes / Q000032:analysis; D006825:Hybridomas; D007143:Immunoglobulin Heavy Chains / Q000737:chemistry* / Q000276:immunology; D007147:Immunoglobulin Light Chains / Q000737:chemistry* / Q000276:immunology; D007135:Immunoglobulin Variable Region / Q000737:chemistry*; D008969:Molecular Sequence Data; D010748:Phosphopeptides / Q000138:chemical synthesis / Q000276:immunology*; D010766:Phosphorylation; D019000:Phosphotyrosine / Q000032:analysis; D016133:Polymerase Chain Reaction; D014746:Vimentin / Q000737:chemistry / Q000276:immunology*
D000911:Antibodies, Monoclonal; D017931:DNA Primers; D000939:Epitopes; D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D007135:Immunoglobulin Variable Region; D010748:Phosphopeptides; D014746:Vimentin; D019000:Phosphotyrosine