A sandwich enzyme-linked immunosorbent assay for human seminal gamma-glutamyl transpeptidase.
A sandwich enzyme-linked immunosorbent assay (ELISA) was developed for detecting human seminal gamma-glutamyl transpeptidase (gamma-GTP) using a combination of anti-seminal gamma-GTP monoclonal antibodies. These monoclonal antibodies did not react with human ovary or uterus in immunohistochemical study. Optimal assay condition, resulting in a sensitive assay with a low background, is presented. The detection limit of this assay was estimated to be 1 ng/ml of seminal gamma-GTP corresponding to about 100,000 times dilution of seminal sample. This ELISA was specific for seminal gamma-GTP, without cross-reactivity to renal or hepatic gamma-GTP, normal blood serum, non-coital vaginal fluid or saliva. The recovery of seminal gamma-GTP added to various biological fluids were also examined.
Abe
S
S
Department of Legal Medicine, Fukushima Medical College, Japan.
Gunji
H
H
Fujita
T
T
Hiraiwa
K
K
D000911:Antibodies, Monoclonal; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005554:Forensic Medicine; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D012661:Semen / Q000166:cytology / Q000201:enzymology*; D005723:gamma-Glutamyltransferase / Q000032:analysis*
D000911:Antibodies, Monoclonal; D005723:gamma-Glutamyltransferase