[Comparison of different methods for the diagnosis of freemartinism--blood group serology, cytology and polymerase chain reaction].
The percentage of freemartins among blood samples tested by chromosome analysis amounted to 83.9%, by blood group serologie 71.4%. 126 blood samples have been tested by blood group serology and PCR. Employing blood group serology, 71.3% and using PCR with BOV97M primers 85.8% of the animals proved to be freemartins. 40 blood samples were additionally analysed using PCR with zinc-finger-gene primers. 36 animals (90%) were identified as being freemartins by means of BOV97M and 34 animals (85%) by means of the zinc-finger-gene primer. The PCR method proved to be a rapid and very sensitive method for the diagnosis of freemartins and also suitable for routine testing. The BOV97M primer showed to have a higher Y chromosome specificity than the zinc-finger-gene primer.
Justi
A
A
Tierärztliches Institut der Georg-August-Universität Göttingen.
Hecht
W
W
Herzog
A
A
Speck
J
J
D000818:Animals; D001789:Blood Group Antigens / Q000235:genetics*; D001788:Blood Grouping and Crossmatching / Q000662:veterinary*; D002417:Cattle; D003582:Cytogenetics; D016172:DNA Fingerprinting / Q000662:veterinary; D005260:Female; D005611:Freemartinism / Q000097:blood / Q000175:diagnosis* / Q000235:genetics; D008297:Male; D016133:Polymerase Chain Reaction / Q000662:veterinary*; D014960:X Chromosome; D014998:Y Chromosome
D001789:Blood Group Antigens