title: Peripheral blood lymphocyte adhesion molecule deployment in the immune response.
abstract: Using precise and reproducible flow cytometric measurements, the surface densities of the cell adhesion molecules (CAMs) CD29, CD2 and CD11a were studied on the CAMhigh (primed) subsets of peripheral blood CD4+ and CD8+ lymphocytes in 56 healthy subjects; 18 patients with acute bacterial infections, 19 with acute viral infections and 18 with chronic inflammatory conditions. By Mann-Whitney analysis, with significant P values adjusted for multiple comparisons to less than 0.0007, patients with viral infections were found to have increased CD11a on CAMhigh cells (an increase in median values of 13.7% for CD4+ lymphocytes and 15.8% for CD8+ lymphocytes); patients with chronic conditions have increased CD29 on CD8+CAMhigh lymphocytes (an increase in the median of 19%); and patients with bacterial infections have increased CD2 on CD8+CAMhigh cells (an increase in the median of 8%). There were marked individual increases in CD29 density: eight (15%) patients had CD29 gender-adjusted density on CD4+ cells greater than the control mean + 3 standard deviations (SD). CD29 densities on CD8+ cells were elevated to greater than control mean + 3 SD in 12 (22%) patients. By multiple regression analysis CD11a density on CD8+ and CD4+ cells and CD2 density on CD4+ cells were found to be associated with HLA-DR expression, but not with CD25 expression. Using standardized intercepts the authors demonstrated that there are very few circulating CDllahighCD25+ cells, suggesting that these are rapidly extravasated. This study demonstrates that in disease, lymphocyte adhesion molecules are not deployed in concert and there are characteristic deployment patterns for different types of immune response.
authors.affiliation: Department of Immunology, University Hospital, Nottingham, UK.
authors.forename: M
authors.initials: M
authors.lastname: Pallis
authors.forename: R A
authors.initials: RA
authors.lastname: Robins
authors.forename: R J
authors.initials: RJ
authors.lastname: Powell
mesh_terms: D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001424:Bacterial Infections / Q000276:immunology; D018845:CD11 Antigens / Q000032:analysis; D018801:CD2 Antigens / Q000032:analysis; D015815:Cell Adhesion Molecules / Q000032:analysis* / Q000276:immunology / Q000502:physiology; D002908:Chronic Disease; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D019012:Integrin beta1 / Q000032:analysis; D008214:Lymphocytes / Q000737:chemistry* / Q000502:physiology*; D008297:Male; D008875:Middle Aged; D015375:Receptors, Interleukin-2 / Q000032:analysis; D012044:Regression Analysis; D015203:Reproducibility of Results; D014777:Virus Diseases / Q000276:immunology
chemical_list: D018845:CD11 Antigens; D018801:CD2 Antigens; D015815:Cell Adhesion Molecules; D019012:Integrin beta1; D015375:Receptors, Interleukin-2