A comparison between glutaraldehyde and diepoxide-fixed stentless porcine aortic valves: biochemical and mechanical characterization and resistance to mineralization.
As previously reported, we found that the fixation rate and thermal denaturation (shrink) temperature of the diepoxide-fixed tissue could be controlled by varying the concentration of the fixative as well as by adding alcohol and/or sodium chloride to the solution. In contrast to prior experience, however, we now found that the epoxide-fixed leaflets exhibited significantly higher resistance to mineralization compared to controls, but only when the tissue had not been exposed to phosphate ion.
Myers
D J
DJ
Medtronic Heart Valves, CA 92714, USA.
Nakaya
G
G
Girardot
M N
MN
Christie
G W
GW
D000818:Animals; D001021:Aortic Valve; D001669:Biochemical Phenomena; D001671:Biochemistry; D001696:Biomechanical Phenomena; D001705:Bioprosthesis; D002114:Calcinosis / Q000517:prevention & control; D003432:Cross-Linking Reagents / Q000737:chemistry; D004853:Epoxy Resins / Q000737:chemistry; D000431:Ethanol / Q000737:chemistry; D005404:Fixatives; D005976:Glutaral / Q000737:chemistry; D006350:Heart Valve Prosthesis; D008297:Male; D010710:Phosphates / Q000737:chemistry; D011475:Prosthesis Failure; D051381:Rats; D017207:Rats, Sprague-Dawley; D012965:Sodium Chloride / Q000737:chemistry; D013499:Surface Properties; D013552:Swine; D013696:Temperature; D014021:Tissue Preservation
D003432:Cross-Linking Reagents; D004853:Epoxy Resins; D005404:Fixatives; D010710:Phosphates; C035364:Quetol 651; D000431:Ethanol; D012965:Sodium Chloride; D005976:Glutaral