4-Picoline-2,2':6',2"-terpyridine-platinum(II) - a potent intercalator of DNA.
4-Picoloine-2,2':6',2"-terpyridine-platinum(II) is shown in a ligation assay to unwind and so intercalate into DNA. Circular dichroism is used to determine an equilibrium binding constant of approximately 2 x 10(7) M(-1) for the most stable binding mode of 4-picoline-2,2':6',2"-terpyridine-platinum(II) to poly[d(A-T)2] with a site size of about 4 base pairs, and about 1 x 10(6) M(-1) for a second binding mode with a site size of about 2 base pairs. Fluorescence spectroscopy provides further evidence for the strong equilibrium binding constant of 4-picoline-2,2':6',2"-terpyridine-platinum(II) in that it displaces ethidium bromide bound to DNA. The double positive charge on 4-picoline-2,2':6',2"-terpyridine-platinum(II), together with the intercalative binding mode is probably responsible for the large binding constant.
McCoubrey
A
A
Dyson Perrins Laboratory, Oxford University, UK.
Latham
H C
HC
Cook
P R
PR
Rodger
A
A
Lowe
G
G
D001665:Binding Sites; D002942:Circular Dichroism; D004247:DNA / Q000378:metabolism*; D004270:DNA, Circular / Q000378:metabolism; D004996:Ethidium; D007364:Intercalating Agents / Q000138:chemical synthesis / Q000378:metabolism*; D009691:Nucleic Acid Denaturation; D009944:Organoplatinum Compounds / Q000138:chemical synthesis / Q000378:metabolism*; D011067:Poly dA-dT / Q000378:metabolism; D013050:Spectrometry, Fluorescence
C098411:4-picoline-2,2'-6',2''-terpyridine-platinum(II); D004270:DNA, Circular; D007364:Intercalating Agents; D009944:Organoplatinum Compounds; D011067:Poly dA-dT; D004247:DNA; D004996:Ethidium