[Mechanisms of the early effect of biologically active hydroxysterols: calcitriol and ecdysterone. Modulation of intracellular pools of arachidonic acid and products of its oxidative metabolism].
It is established that ecdysterone (20-hydroxyecdysone) in concentration of 10(-8) M and calcitriol (1 alpha, 25-dihydroxyvitamin D3) in concentration 10(-12) M evoke in the studied tissues-targets of rats (brain, heart, liver, intestine mucosa) rapid (0.5-2 min) transit (2.5-4 times) increase of the intracellular pools of arachidonic acid, eicosanoid--total peptidoleukotrienes C4/D4/E4 (2.5-6 times), and, especially prostaglandins--stable metabolite prostacyclin 6-ketoprostaglandin F1 alpha (10-400 times). Ecdysterone in concentration 10(-11)-10(-9) inhibits arachidonic cascade in the brain and heart. Activation of arachidonic cascade and further increase of biosynthesis of cGMP are considered as a single negative link of autoregulation of activity of phospholipid-dependent signal system of cells and ecdysterone as a possible efficient modulator intracellular pools of eicosanoid in the tissues of warm-blooded animals.
Kotsiuruba
A V
AV
Bukhanevich
O M
OM
Tuhanova
A V
AV
Tarakanov
S S
SS
D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism*; D002117:Calcitriol / Q000494:pharmacology*; D004441:Ecdysterone / Q000494:pharmacology*; D015777:Eicosanoids / Q000378:metabolism; D015289:Leukotrienes / Q000378:metabolism; D009712:Nucleotides, Cyclic / Q000096:biosynthesis*; D010084:Oxidation-Reduction; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
D015777:Eicosanoids; D015289:Leukotrienes; D009712:Nucleotides, Cyclic; D016718:Arachidonic Acid; D004441:Ecdysterone; D002117:Calcitriol