Inhibitor sensitivity of respiratory complex I in human platelets: a possible biomarker of ageing.
NADH-Coenzyme Q reductase was assayed in platelet mitochondrial membranes obtained from 19 pools of two venous blood samples from female young (19-30 years) individuals and 18 pools from aged ones (66-107 years). The enzyme activities were not significantly changed in the two groups, but a decrease of sensitivity to the specific inhibitor, rotenone, occurred in a substantial number of aged individuals. The results are in agreement with the predictions of the mitochondrial theory of ageing and may be used to develop a sensitive biomarker of the ageing process.
Merlo Pich
M
M
Department of Biochemistry 'G. Morruzzi', University of Bologna, Italy.
Bovina
C
C
Formiggini
G
G
Cometti
G G
GG
Ghelli
A
A
Parenti Castelli
G
G
Genova
M L
ML
Marchetti
M
M
Semeraro
S
S
Lenaz
G
G
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000502:physiology*; D015415:Biomarkers; D001792:Blood Platelets / Q000502:physiology*; D042967:Electron Transport Complex I; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005260:Female; D006801:Humans; D008928:Mitochondria / Q000502:physiology; D009247:NADH, NADPH Oxidoreductases / Q000037:antagonists & inhibitors*; D012402:Rotenone / Q000494:pharmacology*
D015415:Biomarkers; D004791:Enzyme Inhibitors; D012402:Rotenone; D009247:NADH, NADPH Oxidoreductases; D042967:Electron Transport Complex I