title: [Detection of genetically engineered plants by polymerase chain reaction (PCR) using the FLAVR SAVR tomato as an example].
abstract: In this study, a practical approach for the detection of a genetically engineered tomato is demonstrated by polymerase chain reaction (PCR) assays. The Flavr Savr tomato available on the market which was used as an example, contains a resistance gene for the antibiotic kanamycin (kanr) and a gene construct for the inhibition of fruit ripening and softening (antisense polygalacturonase). The presence of these DNA's could only be detected in the genetically engineered tomato.
authors.affiliation: Universität Bern, Institut für Biochemie, Schweiz.
authors.forename: R
authors.initials: R
authors.lastname: Meyer
mesh_terms: D001483:Base Sequence; D017931:DNA Primers; D005818:Genetic Engineering; D007613:Kanamycin Resistance / Q000235:genetics*; D018551:Solanum lycopersicum / Q000235:genetics*; D008969:Molecular Sequence Data; D030821:Plants, Genetically Modified; D011096:Polygalacturonase / Q000096:biosynthesis / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D013025:Glycine max / Q000235:genetics
chemical_list: D017931:DNA Primers; D011096:Polygalacturonase