Specific staining of glycine N-methyltransferase.
Glycine N-methyltransferase from rabbit, human, rat and pig livers was separated by isoelectric focusing and a specific functional staining method was developed through the detection of sarcosine produced from the methylation of glycine. Isozyme patterns obtained in the various species tested differ both in the number of bands and apparent isoelectric points. These differences may explain the contradictory data on the subunit structure and glycosylation status of the enzyme reported so far.
Santos
F
F
Instituto de Antropologia, Faculdade de Ciências, Universidade do Porto, Portugal.
Amorim
A
A
Kömpf
J
J
D000818:Animals; D005998:Glycine / Q000378:metabolism; D050938:Glycine N-Methyltransferase; D006801:Humans; D007525:Isoelectric Focusing / Q000379:methods*; D007527:Isoenzymes / Q000302:isolation & purification* / Q000378:metabolism; D008099:Liver / Q000201:enzymology*; D008745:Methylation; D008780:Methyltransferases / Q000302:isolation & purification* / Q000378:metabolism; D011817:Rabbits; D051381:Rats; D012521:Sarcosine / Q000032:analysis / Q000378:metabolism; D013194:Staining and Labeling; D013552:Swine
D007527:Isoenzymes; D008780:Methyltransferases; D050938:Glycine N-Methyltransferase; D005998:Glycine; D012521:Sarcosine