title: Cord blood transplantation and the potential for gene therapy. Gene transduction using a recombinant adeno-associated viral vector.
abstract: Cord blood, which contains a high frequency of immature stem/progenitor cells with extensive proliferative and replating capacity in vitro was used as a clinical source of transplantable stem and progenitor cells. These cells can be efficiently transduced with new genetic material by using AAV or retroviral vectors. Using a recombinant AAV vector, high level expression of the lacZ gene under a CMV promoter was demonstrated in immature subsets of cord blood progenitor cells.
authors.affiliation: Department of Medicine (Hematology/Oncology), Walther Oncology Center, Indianapolis, Indiana 46202-5121, USA.
authors.forename: H E
authors.initials: HE
authors.lastname: Broxmeyer
authors.forename: S
authors.initials: S
authors.lastname: Cooper
authors.forename: M
authors.initials: M
authors.lastname: Etienne-Julan
authors.forename: X S
authors.initials: XS
authors.lastname: Wang
authors.forename: S
authors.initials: S
authors.lastname: Ponnazhagan
authors.forename: S
authors.initials: S
authors.lastname: Braun
authors.forename: L
authors.initials: L
authors.lastname: Lu
authors.forename: A
authors.initials: A
authors.lastname: Srivastava
mesh_terms: D004274:DNA, Recombinant; D000229:Dependovirus / Q000235:genetics*; D005312:Fetal Blood / Q000166:cytology*; D015870:Gene Expression; D018014:Gene Transfer Techniques; D015316:Genetic Therapy; D005822:Genetic Vectors; D006098:Granulocytes / Q000378:metabolism; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000378:metabolism; D006801:Humans; D007231:Infant, Newborn; D001616:beta-Galactosidase / Q000235:genetics
chemical_list: D004274:DNA, Recombinant; D001616:beta-Galactosidase