title: Effects of acute fluoxetine on extracellular serotonin levels in the raphe: an in vivo microdialysis study.
abstract: Acute administration of fluoxetine (1, 10 and 20 mg/kg i.p.) increased extracellular levels of serotonin (5-hydroxytryptamine, 5-HT) in the frontal cortex, ventral hippocampus and raphe nuclei as measured by in vivo microdialysis in anaesthetized rats. In the frontal cortex, fluoxetine showed a marked dose-response effect whereas in the ventral hippocampus and raphe nuclei the fluoxetine-induced effect was maximum at 10 mg/kg. However, the maximal increase in 5-HT was observed in the cell body-containing area, the raphe nuclei. The order of changes in extracellular 5-HT was raphe nuclei > ventral hippocampus > frontal cortex. Our results add further arguments in favour of the key role played by raphe nuclei in the mechanism of action of serotoninergic antidepressant drugs.
authors.affiliation: Faculté de Pharmacie, Laboratorie de Pharmacologie JE 92-372, Chatenay-Malabry, France.
authors.forename: I
authors.initials: I
authors.lastname: Malagié
authors.forename: A C
authors.initials: AC
authors.lastname: Trillat
authors.forename: C
authors.initials: C
authors.lastname: Jacquot
authors.forename: A M
authors.initials: AM
authors.lastname: Gardier
mesh_terms: D000818:Animals; D004305:Dose-Response Relationship, Drug; D005473:Fluoxetine / Q000494:pharmacology*; D005625:Frontal Lobe / Q000187:drug effects* / Q000378:metabolism; D006624:Hippocampus / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D017551:Microdialysis; D011903:Raphe Nuclei / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012701:Serotonin / Q000378:metabolism*
chemical_list: D005473:Fluoxetine; D012701:Serotonin