[Activity of superoxide dismutase in erythrocytes and leukocytes and levels of zinc and copper in blood of patients with diabetes. Effect of diabetic treatment on examined parameters].
The purpose of the present work was to assess the relationship of leukocyte and erythrocyte superoxide dismutase activity to its cofactors concentrations i.e. zinc and copper in plasma and erythrocyte in diabetic patients and treatment variability. 104 patients were included in the study. 23 persons were in the control group. All patients were divided into 2 groups (NIDDM and IDDM). Patients with NIDDM were divided into 3 subgroups depending on treatment (insulin, gliclazide, dietary treated). In all groups, there were assessed following parameters: the leucocyte and erythrocyte SOD activity according to the method of Misra and Fridovich, and zinc and copper concentrations in plasma and erythrocyte, which were measured by flame absorption spectrophotometry. Statistical analysis was performed using the CRISP program.


CONCLUSIONS
1. The leukocyte and erythrocyte superoxide dismutase activity is significantly lowered in diabetes mellitus. 2. In diabetic patients both in type I and type II as in the healthy people, there is a close correlation between SOD activity and its cofactors i.e. zinc and copper erythrocyte concentrations. 3. Insulin and gliclazide treatment increases SOD activity and delays late diabetic complications.
Zbrońska
H
H
Katedra i Klinika Chorób Wewnetrznych i Zawodowych w Zabrzu.
Grzeszczak
W
W
Jendryczko
A
A
Zbroński
R
R
Kuźniewicz
R
R
D000293:Adolescent; D000328:Adult; D002648:Child; D003300:Copper / Q000097:blood*; D003920:Diabetes Mellitus / Q000097:blood* / Q000628:therapy*; D003927:Diet, Diabetic; D004912:Erythrocytes / Q000201:enzymology; D005260:Female; D005907:Gliclazide / Q000627:therapeutic use; D006801:Humans; D007004:Hypoglycemic Agents / Q000627:therapeutic use; D007328:Insulin / Q000627:therapeutic use; D007962:Leukocytes / Q000201:enzymology; D008297:Male; D008875:Middle Aged; D013482:Superoxide Dismutase / Q000097:blood*; D015032:Zinc / Q000097:blood*
D007004:Hypoglycemic Agents; D007328:Insulin; D003300:Copper; D013482:Superoxide Dismutase; D005907:Gliclazide; D015032:Zinc