Rapid secretion of intracellularly pre-stored interleukin-8 from rabbit platelets upon activation.
Freshly isolated rabbit platelets contained a substantial amount of intracellular interleukin-8 (IL-8) as revealed by immunohistochemistry and Western blotting analysis. Thrombin stimulation at room temperature induced platelets to secrete IL-8 rapidly, and the maximal secretion was observed within 10 min after the activations. Secreted IL-8 possessed neutrophil chemotactic activity that was neutralized by a specific neutralizing monoclonal antibody to rabbit IL-8 but not a control one. These results suggest that rabbit platelets store biologically active IL-8 intracellularly and secrete it rapidly upon activation. This study implies that platelets may be a pivotal participant in inflammatory reactions in rabbits through producing cytokines and chemokines, including IL-8.
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Department of Pharmacology, Cancer Research Institute, Kanazawa University, Ishikawa, Japan.
Mukaida
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N
Matsushima
K
K
D000818:Animals; D001792:Blood Platelets / Q000378:metabolism*; D015153:Blotting, Western; D002478:Cells, Cultured; D007150:Immunohistochemistry; D016209:Interleukin-8 / Q000276:immunology / Q000378:metabolism*; D015539:Platelet Activation; D011817:Rabbits; D013917:Thrombin / Q000502:physiology; D013997:Time Factors
D016209:Interleukin-8; D013917:Thrombin