Effect of coculturing spermatozoa with oviductal cells on the incidence of polyspermy in pig in vitro fertilization.
It is known that oviductal cells play an important role in fertilization in vivo. We were interested in the effect of those cells on spermatozoa and their influence on the incidence of polyspermy in pig in vitro fertilization (IVF) when cocultured with spermatozoa. Oviductal cells are believed to select a highly fecund population of spermatozoa. By coculturing spermatozoa with oviductal cells it is possible to reduce the number of spermatozoa confronting the eggs at fertilization, reducing the incidence of polyspermy. In our study, spermatozoa were cocultured with oviductal cells for 30 min so that they could bind to the oviductal cells. Both bound and unbound spermatozoa were used for fertilization. The results show that the spermatozoa that were bound to the oviductal cells were capable of fertilizing the eggs, but the nonbound spermatozoa had a reduced penetration incidence. With the bound sperm, polyspermy incidence decreased and the two-pronuclei proportion increased. We also found that with time the spermatozoa released from the cells had better motility than those that did not bind. Therefore, it is our belief that oviductal cells can be used for porcine IVF resulting in a lower polyspermy incidence and higher pronuclei incidence.
Dubuc
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Département des sciences animales, Université Laval, Québec, Canada.
Sirard
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MA
D000818:Animals; D002469:Cell Separation; D002478:Cells, Cultured; D005187:Fallopian Tubes / Q000166:cytology* / Q000502:physiology; D005260:Female; D005307:Fertilization in Vitro; D008297:Male; D013094:Spermatozoa / Q000166:cytology* / Q000502:physiology; D013552:Swine; D013997:Time Factors