Guinea pig serum L-asparaginase: purification, and immunological relationship to liver L-asparaginase and serum L-asparaginases in other mammals.
L-asparaginase, an enzyme used in the treatment of acute lymphocytic leukemia, is found in the serum of only a few mammalian groups, including the guinea pig and its close relatives in the superfamily Cavioidea. This report describes the purification and characterization of L-asparaginase from guinea pig serum. Antiserum against the purified enzyme cross-reacted with sera from other Cavioidean species but not with mouse serum. Relatively weak cross-reaction with unpurified L-asparaginase in guinea pig liver indicates a significant degree of evolutionary divergence.
Zhang
N
N
Faculty of Science and Technology, Griffith University, Nathan, Brisbane, Qld, Australia.
Clarke
F
F
Di Trapani
G
G
Keough
D
D
Beacham
I
I
D000818:Animals; D001215:Asparaginase / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D015153:Blotting, Western; D006168:Guinea Pigs / Q000276:immunology / Q000378:metabolism*; D007106:Immune Sera; D008099:Liver / Q000201:enzymology*; D008322:Mammals / Q000276:immunology / Q000378:metabolism*; D051379:Mice
D007106:Immune Sera; D001215:Asparaginase