Effect of penicillin G on the electroporation of Rhodococcus rhodochrous CF222.
Suitable conditions for the introduction of bacteriophage DNA into cells of Rhodococcus rhodochrous CF222 by electroporation were established, and penicillin G was found to enhance the transfection frequency. When conditions optimal for the parental strain were applied to its colony-morphological mutants, different transfection frequencies were observed. Penicillin G enhanced the transfection frequency of smooth and mucoidal mutants but not of rough mutants.
Sunairi
M
M
Department of Applied Biological Science, College of Agriculture and Veterinary Medicine, Nihon University, Kanagawa, Japan.
Iwabuchi
N
N
Murakami
K
K
Watanabe
F
F
Ogawa
Y
Y
Murooka
H
H
Nakajima
M
M
D001435:Bacteriophages / Q000235:genetics; D018274:Electroporation / Q000379:methods*; D005069:Evaluation Studies as Topic; D009154:Mutation; D010400:Penicillin G / Q000494:pharmacology*; D012240:Rhodococcus / Q000187:drug effects* / Q000235:genetics* / Q000254:growth & development; D014162:Transfection / Q000187:drug effects*
D010400:Penicillin G