Determination of metronidazole in vaginal tissue by high-performance liquid chromatography using solid-phase extraction.
A sensitive high-performance liquid chromatographic (HPLC) method for the determination of metronidazole in vaginal tissue is reported. The method uses a Zorbax SB phenyl column with a 0.01 M aqueous monobasic potassium phosphate buffer (pH 4.0)-absolute methanol (85:15, v/v) as mobile phase at a flow-rate of 1.0 ml/min and detection at 313 nm. Tinidazole was used as the internal standard. The method employed homogenization of tissue followed by solid-phase extraction. The quantitation was achieved within 30 min with sensitivity in the ng/g range. Metronidazole was linear in the 100-2000 ng/g range. The accuracy and precision were in the 1-4% range for the drug and the limit of detection was approximately 100 ng/g based on a signal-to-noise ratio of 3 and a 1009-microliters injection.
Venkateshwaran
T G
TG
Department of Medicinal Chemistry, College of Pharmacy, University of Georgia, Athens 30602-2352, USA.
Stewart
J T
JT
D000818:Animals; D000994:Antitrichomonal Agents; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000706:statistics & numerical data; D004285:Dogs; D005260:Female; D006801:Humans; D008795:Metronidazole / Q000008:administration & dosage / Q000032:analysis*; D008832:Microchemistry; D012680:Sensitivity and Specificity; D012995:Solubility; D014621:Vagina / Q000737:chemistry*; D014867:Water
D000994:Antitrichomonal Agents; D014867:Water; D008795:Metronidazole