The glycoprotein of viral hemorrhagic septicemia virus (VHSV): antigenicity and role in virulence.
In order to study the antigenic structure of the G protein of VHSV, we produced several anti-G monoclonal antibodies (MAbs) and used 4 neutralizing MAbs (NMAbs) to select resistant (MAR) mutants. Each MAR mutant was confronted with the 4 NMAbs in a neutralization test, and also with our panel of MAbs in surface plasmon resonance (SPR) analysis to determine the extent of their relatedness. Determination of the sequence of the entire G gene of representative MAR mutants allowed us to map the mutations responsible for the resistant phenotypes. We identified several locations on the G protein sequence, which represent, most probably, critical positions within the binding sites of the neutralizing MAbs. In addition, the MAR mutants selected with a cross-reactive MAb exhibited a reduced pathogenicity for fish. This indicated that the regions bearing the point mutations selected with MAb C10 were probably involved in the determination of the virulent phenotype.
Béarzotti
M
M
INRA, unité de virologie et immunologie moléculaires, Jouy-en-Josas, France.
Monnier
A F
AF
Vende
P
P
Grosclaude
J
J
de Kinkelin
P
P
Benmansour
A
A
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D000956:Antigens, Viral / Q000276:immunology; D003001:Cloning, Molecular; D003062:Codon / Q000235:genetics; D005393:Fish Diseases; D016297:Mutagenesis, Site-Directed; D009500:Neutralization Tests; D017686:Oncorhynchus mykiss; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D012209:Rhabdoviridae / Q000276:immunology* / Q000472:pathogenicity; D018353:Rhabdoviridae Infections / Q000503:physiopathology / Q000662:veterinary; D013997:Time Factors; D014759:Viral Envelope Proteins / Q000276:immunology*; D014774:Virulence
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D003062:Codon; C096089:G protein, viral hemorrhagic septicemia virus; D014759:Viral Envelope Proteins