Quantification and molecular analysis of plasmatic HLA-BCw molecules with a locus B and Cw HLA specific mAb (B1.23.2 mAb).
Plasmatic HLA class I molecules were analysed quantitatively and qualitatively with a B and Cw locus-specific mAb, B1.23.2. sHLA-BCw were detected among all individuals with a wide range of concentration. The HLA-A29(19) allele was significantly associated with an increased sHLA-BCw mean concentration. Three different isoforms of relative molecular weights of 44, 39 and 36kDa were detected. The 39 kDa class I heavy chain was not detected after immunoprecipitation with mAb B1.23.2 in 17% of tested plasma, but was immunoprecipitated with W6/32 mAb.
Drouet
M
M
Laboratoire d'Immunologie, CHRU Dupuytren, Limoges, France.
Aussel
L
L
Drenou
B
B
Fauchet
R
R
D000483:Alleles; D000911:Antibodies, Monoclonal / Q000276:immunology; D015153:Blotting, Western; D015234:HLA-A Antigens / Q000235:genetics; D015235:HLA-B Antigens / Q000097:blood* / Q000276:immunology; D015236:HLA-C Antigens / Q000097:blood* / Q000276:immunology; D006801:Humans; D007518:Isoantibodies / Q000276:immunology; D008970:Molecular Weight; D011233:Precipitin Tests; D012995:Solubility
D000911:Antibodies, Monoclonal; D015234:HLA-A Antigens; C035524:HLA-A29 antigen; D015235:HLA-B Antigens; D015236:HLA-C Antigens; D007518:Isoantibodies