title: Peroxisomal beta-oxidation activity and catalase activity during development and aging in mouse liver.
abstract: Liver peroxisomal beta-oxidation activity on stearate, oleate, linoleate and alpha-linolenate was investigated as a function of age in two mouse strains. Each fatty acid showed a similar beta-oxidation activity pattern with age characterized by a rapid increase (200%) from day 2 to 20 followed by a dramatic weaning-related decrease (70%) from day 20 to 22. There was a new increase (260%) from day 22 to 75, then a plateau up to day 300 days, and finally an age-related decrease (70%) from day 300 to 540. Oleic, linoleic and alpha-linolenic acids were respectively 5-, 7.5- and 9-fold more degraded than stearic acid. Catalase specific activity showed the same age-related pattern as fatty acid beta-oxidation. Both mouse strains showed the age-related decreases. The longer-lived strain exhibited higher activity for both peroxisomal beta-oxidation and catalase and the rate of decrease of these two activities during aging was the same for both strains. The catalase/alpha-linolenic acid beta-oxidation ratio was constant during adulthood and aging. These results suggest that peroxisomal beta-oxidation and catalase activities are closely related throughout and implications for long-chain and very long-chain fatty acid metabolism, maintenance of membrane fatty acid composition and anti-oxidant status during aging are discussed.
authors.affiliation: Laboratoire de Neurotoxiologie, INSERM U26, Hôpital Fernand Widal, Paris, France.
authors.forename: R
authors.initials: R
authors.lastname: Périchon
authors.forename: J M
authors.initials: JM
authors.lastname: Bourre
mesh_terms: D000367:Age Factors; D000818:Animals; D002374:Catalase / Q000187:drug effects / Q000378:metabolism*; D005260:Female; D006861:Hydrogen Peroxide / Q000378:metabolism / Q000494:pharmacology; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000378:metabolism; D008099:Liver / Q000201:enzymology* / Q000254:growth & development*; D051379:Mice; D008815:Mice, Inbred Strains; D008830:Microbodies / Q000201:enzymology* / Q000378:metabolism*; D019301:Oleic Acid; D009829:Oleic Acids / Q000378:metabolism; D010084:Oxidation-Reduction; D013228:Stearates / Q000378:metabolism; D013997:Time Factors; D017962:alpha-Linolenic Acid / Q000378:metabolism
chemical_list: D008041:Linoleic Acids; D009829:Oleic Acids; D013228:Stearates; D017962:alpha-Linolenic Acid; D019301:Oleic Acid; D019787:Linoleic Acid; D006861:Hydrogen Peroxide; D002374:Catalase