title: The regulator of streptomycin gene expression, StrR, of Streptomyces griseus is a DNA binding activator protein with multiple recognition sites.
abstract: In Streptomyces griseus the expression of at least one streptomycin biosynthetic gene, strB1, is dependent on the pathway-specific activator protein StrR. We show here that StrR is a DNA-binding protein which specifically interacts with the strB1 promoter fragment. Footprinting experiments demonstrate that the StrR protein binds to an inverted repeat located upstream of the strB1 promoter. Further StrR-binding sites having the consensus sequence GTTCGActG(N)11CagTcGAAc were identified in the str-sts gene clusters of S. griseus and Streptomyces glaucescens by sequence comparison, gel retardation, and footprinting studies. The genetic and biochemical evidence strongly supports the model of the StrR protein activating the expression of streptomycin biosynthetic genes by interacting with multiple binding sites within the str-sts gene clusters of S. griseus and S. glaucescens.
authors.affiliation: Institut für Chemische Mikrobiologie, Bergische Universität GH Wuppertal, Germany.
authors.forename: L
authors.initials: L
authors.lastname: Retzlaff
authors.forename: J
authors.initials: J
authors.lastname: Distler
mesh_terms: D001426:Bacterial Proteins; D001483:Base Sequence; D001665:Binding Sites; D017124:Conserved Sequence; D018983:DNA Footprinting; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D003850:Deoxyribonuclease I / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D008969:Molecular Sequence Data; D005810:Multigene Family; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics*; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000302:isolation & purification; D012091:Repetitive Sequences, Nucleic Acid; D013045:Species Specificity; D013305:Streptomyces griseus / Q000235:genetics* / Q000378:metabolism; D013307:Streptomycin / Q000096:biosynthesis*; D014157:Transcription Factors / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D011993:Recombinant Fusion Proteins; C098255:StrR protein, Streptomyces griseus; D014157:Transcription Factors; D017853:Phosphotransferases (Alcohol Group Acceptor); C022680:streptomycin 6-kinase; D003850:Deoxyribonuclease I; D013307:Streptomycin