title: Plasma/muscle ratios of sulfadimethoxine residues in channel catfish (Ictalurus punctatus).
abstract: Channel catfish (n = 84) maintained at a water temperature of 27 degrees C were used in a feeding study to determine the plasma to muscle concentration ratios of sulfadimethoxine (SDM) and 4-N-acetylsulfadimethoxine residues. Sulfadimethoxine medicated feed was provided free choice at 42 mg SDM/kg body weight once daily for 5 days and the plasma and muscle concentrations of SDM were determined at selected withdrawal times (6, 12, 24, 48, 72, and 96 hours) following the last dose. Considerable variation in total SDM tissue concentration among fish within a sampling period was observed. For fish (n = 12) at six hours post-dose, total SDM concentrations ranged from 1.4-24.8 micrograms/mL and 0.6-12.6 micrograms/g, with mean total SDM concentrations of 9.1 micrograms/mL and 5.3 micrograms/g for plasma and muscle, respectively. However, a mean plasma:muscle concentration ratio of 1.8:1 +/- 0.3:1 was obtained over all concentrations and sampling periods. The plasma:muscle 95% t distribution interval for individual fish was 1.2:1 to 2.4:1. A correlation coefficient of 0.967 was obtained for the relationship between plasma and muscle total SDM concentration among individual fish (n = 25). Results of this study indicate that plasma total SDM concentration may be used to identify samples containing violative SDM muscle residue. No fish contained total SDM muscle residues greater than the FDA tolerance (0.1 microgram/g) by 48 hours following the final dose.
authors.affiliation: Department of Veterinary Physiology, Pharmacology and Toxicology, Louisiana State University, Baton Rouge 70803-8420, USA.
authors.forename: C C
authors.initials: CC
authors.lastname: Walker
authors.forename: R L
authors.initials: RL
authors.lastname: Thune
authors.forename: S A
authors.initials: SA
authors.lastname: Barker
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000097:blood / Q000493:pharmacokinetics*; D017756:Aquaculture; D002851:Chromatography, High Pressure Liquid; D004350:Drug Residues / Q000493:pharmacokinetics*; D005260:Female; D007059:Ictaluridae / Q000378:metabolism*; D008297:Male; D009132:Muscles / Q000378:metabolism*; D013412:Sulfadimethoxine / Q000031:analogs & derivatives / Q000097:blood / Q000493:pharmacokinetics*
chemical_list: D000890:Anti-Infective Agents; C038180:N(4)-acetylsulfadimethoxine; D013412:Sulfadimethoxine