title: Detection of Brucella abortus antigens by immunoperoxidase histochemical staining of lochia smears.
abstract: An immunoperoxidase histochemical staining technique for the detection of Brucella abortus organisms in lochia of brucellosis-infected cows was described. In comparison with the standard culture technique, the former method resulted in 36% false positive and 2.3% false negative reactions, which represents a sensitivity of 82% and a specificity of 94%. False positive reactions are presumably the result of organisms which succumbed during transit. The feasibility of the test in the confirmation of brucellosis is discussed.
authors.affiliation: Veterinary Laboratory, Kroonstad, South Africa.
authors.forename: J N
authors.initials: JN
authors.lastname: Alberts
authors.forename: J A
authors.initials: JA
authors.lastname: Erasmus
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis*; D002003:Brucella abortus / Q000276:immunology / Q000302:isolation & purification*; D002007:Brucellosis, Bovine / Q000175:diagnosis* / Q000382:microbiology; D002417:Cattle; D007124:Immunoenzyme Techniques; D013019:South Africa
chemical_list: D000942:Antigens, Bacterial