title: The 190 kDa centrosome-associated protein of Drosophila melanogaster contains four zinc finger motifs and binds to specific sites on polytene chromosomes.
abstract: Microinjection of a bacterially expressed, TRITC labelled fragment of the centrosome-associated protein CP190 of Drosophila melanogaster, into syncytial Drosophila embryos, shows it to associate with the centrosomes during mitosis, and to relocate to chromatin during interphase. Indirect immunofluorescence staining of salivary gland chromosomes of third instar Drosophila larvae, with antibodies specific to CP190, indicate that the protein is associated with a large number of loci on these interphase polytene chromosomes. The 190 kDa CP190 protein is encoded by a 4.1 kb transcript with a single, long open reading frame specifying a polypeptide of 1,096 amino acids, with a molecular mass of 120 kDa, and an isoelectric point of 4.5. The central region of the predicted amino acid sequence of the CP190 protein contains four CysX2CysX12HisX4His zinc-finger motifs which are similar to those described for several well characterised DNA binding proteins. The data suggest that the function of CP190 involves cell cycle dependent associations with both the centrosome, and with specific chromosomal loci.
authors.affiliation: Department of Biological Sciences, University, Dundee, UK.
authors.forename: W G
authors.initials: WG
authors.lastname: Whitfield
authors.forename: M A
authors.initials: MA
authors.lastname: Chaplin
authors.forename: K
authors.initials: K
authors.lastname: Oegema
authors.forename: H
authors.initials: H
authors.lastname: Parry
authors.forename: D M
authors.initials: DM
authors.lastname: Glover
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites / Q000502:physiology; D018385:Centrosome / Q000378:metabolism*; D002843:Chromatin / Q000378:metabolism; D002875:Chromosomes / Q000378:metabolism*; D017124:Conserved Sequence; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000502:physiology*; D004625:Embryo, Nonmammalian / Q000378:metabolism; D019084:Fluorescent Antibody Technique, Indirect; D017344:Genes, Insect / Q000502:physiology; D015726:Giant Cells / Q000378:metabolism; D007399:Interphase / Q000502:physiology; D008845:Microinjections; D008869:Microtubule-Associated Proteins / Q000378:metabolism* / Q000648:ultrastructure; D008938:Mitosis / Q000502:physiology; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000378:metabolism* / Q000648:ultrastructure; D016335:Zinc Fingers / Q000502:physiology*
chemical_list: C107964:CP190 protein, Drosophila; D002843:Chromatin; D029721:Drosophila Proteins; D008869:Microtubule-Associated Proteins; D009687:Nuclear Proteins