Induction of macrophage apoptosis by Bordetella pertussis adenylate cyclase-hemolysin.
We previously demonstrated that Bordetella pertussis induced macrophage apoptosis and that adenylate cyclase-hemolysin expression is required for this phenomenon. In order to determine a role for other B. pertussis factors, such as pertussis toxin or the adhesins, pertactin and filamentous hemagglutinin, we compared mutants deficient in adhesin expression or purified proteins for their ability to induce cell death. We showed that only purified adenylate cyclase-hemolysin, but neither adhesins, nor pertussis toxin, killed macrophages through apoptosis. We also demonstrated that all activities of adenylate cyclase-hemolysin were required. Moreover, our results indicate that B. pertussis does not have to invade cells to be lethal.
Khelef
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Laboratoire des Bordetella, Institut Pasteur, Paris, France.
Guiso
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D037361:Adenylate Cyclase Toxin; D000262:Adenylyl Cyclases / Q000378:metabolism; D018829:Adhesins, Bacterial / Q000494:pharmacology; D000818:Animals; D017209:Apoptosis; D001425:Bacterial Outer Membrane Proteins / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D001886:Bordetella pertussis / Q000737:chemistry*; D004247:DNA / Q000378:metabolism; D006388:Hemagglutinins / Q000494:pharmacology; D006460:Hemolysin Proteins / Q000494:pharmacology*; D008264:Macrophages / Q000166:cytology*; D051379:Mice; D037342:Pertussis Toxin; D011498:Protein Precursors / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000494:pharmacology; D010566:Virulence Factors, Bordetella / Q000494:pharmacology
D037361:Adenylate Cyclase Toxin; D018829:Adhesins, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D006388:Hemagglutinins; D006460:Hemolysin Proteins; D011498:Protein Precursors; D011994:Recombinant Proteins; D010566:Virulence Factors, Bordetella; C068929:pertactin; D004247:DNA; D037342:Pertussis Toxin; D000262:Adenylyl Cyclases