Quantitative analysis of synthetic human calcitonin by liquid chromatography-mass spectrometry.
The quantitative analysis of synthetic human calcitonin (hCT) by micro-liquid chromatography-electrospray ionization mass spectrometry (micro-LC-ESI-MS) is reported. hCT was extracted from plasma by an immobilized antibody column and separated by a micro-LC system. The molecular mass of hCT is 3417, and m/z 1140, corresponding to the [M + 3H]3+ ion, was observed by ESI-MS. This ion was monitored in the selected-ion monitoring mode; rat calcitonin, which is highly homologous to hCT, was used as an internal standard. The calibration curve for the quantification of hCT was linear in the range 10 ng/ml to 1 microgram/ml of plasma.
Kobayashi
N
N
Analytical Laboratory, Sankyo Co., Ltd., Tokyo, Japan.
Kanai
M
M
Seta
K
K
Nakamura
K
K
D000595:Amino Acid Sequence; D000818:Animals; D002116:Calcitonin / Q000097:blood*; D002138:Calibration; D002853:Chromatography, Liquid; D006801:Humans; D007202:Indicators and Reagents; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D051381:Rats; D011994:Recombinant Proteins / Q000097:blood; D012015:Reference Standards
D007202:Indicators and Reagents; D011994:Recombinant Proteins; D002116:Calcitonin