Simple two-step procedure for the preparation of highly active pure equine milk lysozyme.
A fast, simple two-step purification scheme is presented for the isolation of lysozyme at a high yield from equine milk. In the first step, fluidized bed technology, using the Streamline system, was exploited. In the following step, advantage was taken of Ca(2+)-induced conformational changes to obtain a pure, high specific activity, enzyme fraction by hydrophobic interaction chromatography.
Noppe
W
W
Interdisciplinary Research Centre, Katholieke Universiteit Leuven-Campus Kortrijk, Belgium.
Hanssens
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I
De Cuyper
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M
D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002850:Chromatography, Gel / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006736:Horses; D007525:Isoelectric Focusing; D008892:Milk / Q000737:chemistry*; D009113:Muramidase / Q000032:analysis / Q000302:isolation & purification*
D009113:Muramidase; D002118:Calcium