title: Cytolytic activity and associated serine protease expression by skin and afferent lymph CD8+ T cells during orf virus reinfection.
abstract: Following orf virus reinfection in sheep, CD8+ T cells were recruited to the lesion site in the skin during the period of viral replication and appeared in increased numbers in afferent lymph draining the infection site. A proportion of the CD8+ T cells were activated, particularly in the skin, as determined by their expression of the cytolytic cell-associated serine protease, BLT-esterase. This was a useful marker for activated cytolytic cells as there was a good correlation between afferent lymph CD8+ T cell-mediated lysis of autologous orf virus-infected skin cells and their expression of BLT-esterase in vitro. The majority of the cells that expressed BLT-esterase in the skin and lymph were CD8+ T cells and cytolysis in vitro was predominately by the MHC class I antigen presentation pathway. The conclusion is that orf virus reinfection is characterized by limited viral replication and stimulation of an activated CTL response in the skin.
authors.affiliation: Moredum Research Institute, Scotland, UK.
authors.forename: D M
authors.initials: DM
authors.lastname: Haig
authors.forename: G
authors.initials: G
authors.lastname: Hutchinson
authors.forename: J
authors.initials: J
authors.lastname: Thomson
authors.forename: D
authors.initials: D
authors.lastname: Yirrell
authors.forename: H W
authors.initials: HW
authors.lastname: Reid
mesh_terms: D000818:Animals; D003602:Cytotoxicity, Immunologic; D004474:Ecthyma, Contagious / Q000276:immunology*; D005260:Female; D053804:Granzymes; D006412:Hematopoietic Stem Cells / Q000276:immunology; D008196:Lymph / Q000276:immunology*; D008213:Lymphocyte Activation; D012008:Recurrence; D012697:Serine Endopeptidases / Q000378:metabolism*; D012756:Sheep; D012867:Skin / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000201:enzymology / Q000276:immunology*
chemical_list: D053804:Granzymes; D012697:Serine Endopeptidases