In vivo effects of tumor necrosis factor-alpha on incised wound and gunshot wound healing.
Recombinant tumor necrosis factor alpha (TNF-alpha) was applied to incised wounds (adult rats, n = 14) and gunshot wounds (dogs, n = 5). The accumulation of RNA and DNA in regenerative tissue, the wound disruption strength (WDS), and histology were examined. The results showed that a single dose of TNF-alpha in saline with highly purified bovine serum albumin increased the WDS and accumulation of RNA and DNA in regenerative tissue in incised wounds 3 days after surgery (p < 0.05). Also, TNF-alpha promoted the inflammatory reaction in the wounded area of gunshot wounds. Histology revealed increased capillary tube-like structures and collagen formation and fibroblast proliferation in both incised and gunshot wounds treated with TNF-alpha. The results indicated that locally applied TNF-alpha benefited the incised and gunshot wound healing. These mitogenic and inflammatory effects of TNF-alpha may be the synergistic effects with other growth factors such as platelet-derived growth factor (PDGF).
Fu
X
X
Trauma Center of Postgraduate Medical College, 304th Hospital, Beijing, People's Republic of China.
Tian
H
H
Hsu
S
S
Wang
D
D
Sheng
Z
Z
D000818:Animals; D004247:DNA / Q000096:biosynthesis; D004285:Dogs; D008297:Male; D012313:RNA / Q000096:biosynthesis; D051381:Rats; D011994:Recombinant Proteins / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*; D014945:Wound Healing / Q000187:drug effects*; D014948:Wounds, Gunshot / Q000188:drug therapy / Q000473:pathology / Q000503:physiopathology*; D014951:Wounds, Stab / Q000188:drug therapy / Q000473:pathology / Q000503:physiopathology*
D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D012313:RNA; D004247:DNA