The X-linked methylated DNA binding protein, Mecp2, is subject to X inactivation in the mouse.
DNA methylation at the promoter region of X-linked genes is associated with the maintenance of X inactivation in mammals. One of the methylated DNA binding proteins, MECP2, that binds to methylated bases in DNA is encoded by a gene (Mecp2) located on the mouse X Chromosome (Chr). To determine whether this gene was expressed from the inactive X Chr, and X-autosome translocation (T(X;16)16H) system in which expression from the Mecp2 allele on the inactive X Chr could be assayed was used. Results from these experiments indicate that Mecp2 is subject to X inactivation in mouse.
Adler
D A
DA
Department of Pathology, University of Washington, Seattle 98195, USA.
Quaderi
N A
NA
Brown
S D
SD
Chapman
V M
VM
Moore
J
J
Tate
P
P
Disteche
C M
CM
D000818:Animals; D001483:Base Sequence; D002868:Chromosomal Proteins, Non-Histone; D017931:DNA Primers; D004268:DNA-Binding Proteins / Q000235:genetics*; D004303:Dosage Compensation, Genetic; D005260:Female; D008040:Genetic Linkage; D051783:Methyl-CpG-Binding Protein 2; D008745:Methylation; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D009115:Muridae; D012097:Repressor Proteins; D014178:Translocation, Genetic; D014960:X Chromosome
D002868:Chromosomal Proteins, Non-Histone; D017931:DNA Primers; D004268:DNA-Binding Proteins; C497393:Mecp2 protein, mouse; D051783:Methyl-CpG-Binding Protein 2; D012097:Repressor Proteins