Introduction to positional cloning.
The most common procedure for isolating genes that predispose to a certain disease is positional cloning. This process is initiated by mapping the responsible gene to its location on a chromosome linked to the disease. Successive cloning of the region results in the isolation of candidate genes. Finally, identification of mutations by mutational analysis points to the correct gene.
Papadopoulos
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Johns Hopkins Oncology Center, Baltimore, Maryland, USA.
D002874:Chromosome Mapping; D003001:Cloning, Molecular / Q000379:methods*; D004247:DNA / Q000235:genetics; D004252:DNA Mutational Analysis; D018076:DNA, Complementary / Q000235:genetics; D005796:Genes; D030342:Genetic Diseases, Inborn / Q000235:genetics; D005819:Genetic Markers; D005822:Genetic Vectors; D006801:Humans; D008126:Lod Score
D018076:DNA, Complementary; D005819:Genetic Markers; D004247:DNA