title: Filament-specific expression of a cellulase gene in the dimorphic fungus Ustilago maydis.
abstract: The phytopathogenic fungus Ustilago maydis exists in a yeast-like haploid form and as a filamentous dikaryon. Only the dikaryon can infect corn plants. We have isolated a gene, egl1, that is not expressed in haploid cells but strongly induced in the filament. Molecular and biochemical analyses revealed that egl1 encodes a cellulase. By immunogold labelling, secreted protein could be detected at the hyphal tip. Mutants deleted for egl1 are viable and are affected neither in filament formation nor in pathogenic development under the conditions tested.
authors.affiliation: Institut für Genetik und Mikrobiologie, Universität München, Germany.
authors.forename: F
authors.initials: F
authors.lastname: Schauwecker
authors.forename: G
authors.initials: G
authors.lastname: Wanner
authors.forename: R
authors.initials: R
authors.lastname: Kahmann
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D015153:Blotting, Western; D002480:Cellulase / Q000096:biosynthesis / Q000235:genetics*; D000939:Epitopes; D015966:Gene Expression Regulation, Fungal / Q000502:physiology*; D015723:Gene Library; D005800:Genes, Fungal / Q000235:genetics*; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D010957:Plasmids; D014588:Ustilago / Q000201:enzymology / Q000235:genetics* / Q000648:ultrastructure
chemical_list: D000939:Epitopes; D002480:Cellulase