Competitive ELISA for the detection of antibodies against epizootic haemorrhagic disease of deer virus.
A competitive enzyme-linked immunosorbent assay is described for the detection of antibodies against epizootic haemorrhagic disease of deer viruses (EHDV). Test antisera were tested against a guinea-pig antiserum raised against EHDV core particles. The assay detected antibodies to all serotypes of EHDV, but unlike the agar gel immunodiffusion (AGID) test, gave no cross-reactions with antibodies against bluetongue, Palyam and Tilligery viruses. The C-ELISA would be ideal for use in epidemiological surveys since it is suitable for the examination of antisera from all susceptible species without the need for individual species-specific enzyme conjugates.
Thevasagayam
J A
JA
Institute for Animal Health, Woking, Surrey, UK.
Mertens
P P
PP
Burroughs
J N
JN
Anderson
J
J
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood* / Q000276:immunology; D002417:Cattle; D002460:Cell Line; D006224:Cricetinae; D003429:Cross Reactions; D003670:Deer; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006168:Guinea Pigs; D018164:Hemorrhagic Disease Virus, Epizootic / Q000276:immunology / Q000302:isolation & purification*; D006736:Horses; D012088:Reoviridae Infections / Q000175:diagnosis / Q000662:veterinary; D012680:Sensitivity and Specificity; D012756:Sheep; D013997:Time Factors; D014758:Viral Core Proteins / Q000276:immunology*
D000914:Antibodies, Viral; D014758:Viral Core Proteins