Genetic manipulation of polyphosphate metabolism affects cadmium tolerance in Escherichia coli.
The polyphosphate metabolic pathways in Escherichia coli were genetically manipulated to test the effect of polyphosphate on tolerance to cadmium. A polyphosphate kinase (ppk) and polyphosphatase (ppx) mutant strain produced no polyphosphate, whereas the same strain carrying multiple copies of ppk on a high-copy plasmid produced significant quantities. The doubling times of both strains increased with increasing cadmium concentrations. In contrast, the mutant strain carrying multiple copies of ppk and ppx produced 1/20 of the polyphosphate found in the strain carrying multiple copies of ppk only and showed no significant increase in doubling time over the same cadmium concentration range.
Keasling
J D
JD
Department of Chemical Engineering, University of California, Berkeley 94720-1462, USA. keasling@garnet.berkeley.edu
Hupf
G A
GA
D017766:Acid Anhydride Hydrolases / Q000235:genetics; D002104:Cadmium / Q000494:pharmacology*; D004352:Drug Resistance, Microbial / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism*; D005798:Genes, Bacterial; D009154:Mutation; D017856:Phosphotransferases (Phosphate Group Acceptor) / Q000235:genetics; D011122:Polyphosphates / Q000378:metabolism*
D011122:Polyphosphates; D002104:Cadmium; D017856:Phosphotransferases (Phosphate Group Acceptor); C022424:polyphosphate kinase; D017766:Acid Anhydride Hydrolases; C022563:endopolyphosphatase