title: Are mast cells involved in hypertensive heart disease?
abstract: OBJECTIVE
  To evaluate the potential relationship of mast cells with myocardial fibrosis in the cardiac ventricles of spontaneously hypertensive rats (SHR).
  
  
  METHODS
  Experiments were performed on hearts from 36-week-old SHR with established left ventricular hypertrophy (n = 12) and from 36-week-old normotensive Wistar-Kyoto (WKY) rats (n = 12). Furthermore, to evaluate whether antihypertensive treatment with the angiotensin converting enzyme inhibitor quinapril interferes with the potential relationship between mast cells and fibrosis in SHR, we treated 16-week-old SHR (n = 12) with oral quinapril (10 mg/kg body weight per day) for 20 weeks.
  
  
  METHODS
  Mast cells were counted in 25 high-power fields. Toluidine blue-stained sections and avidin staining were used to detect mast cells. The extent of myocardial fibrosis was analysed in samples stained with Masson's trichrome. The amount of collagen was evaluated morphometrically, using an automatic image analyser, and biochemically, using myocardial hydroxyproline concentration.
  
  
  RESULTS
  In the left ventricle of untreated SHR compared with age- and sex-matched normotensive WKY rats we found more extensive interstitial and perivascular fibrosis, an increased collagen volume fraction, an increased hydroxyproline concentration and an increased number of mast cells. Similar but less intense abnormalities were observed in the right ventricles of untreated SHR compared with the left ventricles of the same rats. In the left ventricles of quinapril-treated SHR compared with those of untreated SHR we found a marked decrease in fibrosis, a lower collagen volume fraction, a lower hydroxyproline concentration and fewer mast cells. Treatment with quinapril was also accompanied by normalization in the myocardial structure of the right ventricles of SHR. A positive correlation was found between the density of mast cells and the collagen volume fraction in the left ventricles of all of the rats.
  
  
  CONCLUSIONS
  The present findings suggest that mast cells can play a part in the development of the myocardial fibrosis that occurs in the cardiac ventricles with hypertensive cardiac hypertrophy. In addition, the present results suggest that the ability of quinapril to interfere with mast cells might be involved in its cardioreparative properties.
authors.affiliation: Department of Pathology, University Clinic, School of Medicine, Pamplona, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Panizo
authors.forename: F J
authors.initials: FJ
authors.lastname: Mindán
authors.forename: M F
authors.initials: MF
authors.lastname: Galindo
authors.forename: E
authors.initials: E
authors.lastname: Cenarruzabeitia
authors.forename: M
authors.initials: M
authors.lastname: Hernández
authors.forename: J
authors.initials: J
authors.lastname: Díez
mesh_terms: D000818:Animals; D000959:Antihypertensive Agents / Q000627:therapeutic use; D001794:Blood Pressure; D004719:Endomyocardial Fibrosis / Q000188:drug therapy / Q000473:pathology*; D006973:Hypertension / Q000473:pathology*; D017379:Hypertrophy, Left Ventricular / Q000188:drug therapy / Q000473:pathology; D007546:Isoquinolines / Q000627:therapeutic use; D008297:Male; D008407:Mast Cells / Q000187:drug effects / Q000502:physiology*; D000077583:Quinapril; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D044005:Tetrahydroisoquinolines
chemical_list: D000959:Antihypertensive Agents; D007546:Isoquinolines; D044005:Tetrahydroisoquinolines; D000077583:Quinapril