Uroguanylin: cloning of preprouroguanylin cDNA, mRNA expression in the intestine and heart and isolation of uroguanylin and prouroguanylin from plasma.
Uroguanylin is a small peptide isolated from opossum urine that activates membrane guanylate cyclases. We report the isolation by molecular cloning of cDNAs encoding the 109 amino acid preprouroguanylin containing the active uroguanylin peptide at its C-terminus. Preprouroguanylin mRNAs of 1.2 kb were detected throughout the small and large intestine and in the atria and ventricles of heart, but not in kidney, stomach or liver. Transfection of COS-1 cells with the uroguanylin cDNA resulted in prouroguanylin secretion. Both uroguanylin and prouroguanylin were isolated from opossum plasma. Thus, uroguanylin is made by the intestine and heart and circulates as a bioactive form of uroguanylin and the inactive prouroguanylin.
Fan
X
X
Truman V.A. Medical Center, Columbia, Missouri 65212, USA.
Hamra
F K
FK
Freeman
R H
RH
Eber
S L
SL
Krause
W J
WJ
Lim
R W
RW
Pace
V M
VM
Currie
M G
MG
Forte
L R
LR
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002460:Cell Line; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D018076:DNA, Complementary; D005768:Gastrointestinal Hormones; D015870:Gene Expression; D007413:Intestinal Mucosa / Q000378:metabolism*; D007420:Intestine, Large / Q000378:metabolism; D007421:Intestine, Small / Q000378:metabolism; D008969:Molecular Sequence Data; D009206:Myocardium / Q000378:metabolism*; D045265:Natriuretic Peptides; D009893:Opossums; D009928:Organ Specificity; D010452:Peptide Biosynthesis; D010455:Peptides / Q000302:isolation & purification; D011498:Protein Precursors / Q000096:biosynthesis* / Q000302:isolation & purification / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D014162:Transfection
D018076:DNA, Complementary; D005768:Gastrointestinal Hormones; D045265:Natriuretic Peptides; D010455:Peptides; D011498:Protein Precursors; D012333:RNA, Messenger; D011994:Recombinant Proteins; C083435:preproguanylin; C072685:guanylin; C084362:uroguanylin