Inhibition of S-phase entry of human fibroblasts by an antisense oligomer against hCDC47.
The MCM protein family has been implicated in the regulatory machinery causing DNA to replicate only once in the S phase. Recently, we have identified human (h) CDC47, another member of the family, and demonstrated its unique S-phase nuclear association pattern implying participation in the regulation of DNA replication. In the present study, we applied an antisense strategy to assess the phenotype of human cells in which hCDC47 is repressed. As a result serum-induced accumulation of hCDC47 protein in human fibroblast cells was reduced by a specific antisense oligodeoxynucleotide and S-phase entry of such cells was significantly inhibited, indicating that hCDC47 protein is essential for DNA replication.
Fujita
M
M
Laboratory of Viral Oncology, Research Institute, Aichi Cancer Center, Chikusa-ku, Nagoya, Japan.
Kiyono
T
T
Hayashi
Y
Y
Ishibashi
M
M
D001483:Base Sequence; D002453:Cell Cycle / Q000187:drug effects* / Q000502:physiology; D018797:Cell Cycle Proteins / Q000235:genetics*; D002460:Cell Line; D004268:DNA-Binding Proteins; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D005434:Flow Cytometry; D006801:Humans; D064168:Minichromosome Maintenance Complex Component 7; D008969:Molecular Sequence Data; D009687:Nuclear Proteins; D016376:Oligonucleotides, Antisense / Q000235:genetics / Q000494:pharmacology*; D016196:S Phase / Q000187:drug effects*
D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D016376:Oligonucleotides, Antisense; C101624:MCM7 protein, human; D064168:Minichromosome Maintenance Complex Component 7