Effects of gomisin A on the promotor action and serum bile acid concentration in hepatocarcinogenesis induced by 3'-methyl-4-dimethylamino-azobenzene.
The effects of gomisin A, a lignan component of Schizandra fruits, on the promotion stage of hepatocarcinogenesis initiated by 3'-methyl-4-dimethylamino-azobenzene (3'-MeDAB) in male Donryu rats were investigated. When different types of tumor promotors, phenobarbital (PB) and deoxycholic acid (DCA), were administered for 5 weeks after initiation by 3'-MeDAB, preneoplastic alterations in the liver, determined by glutathione S-transferase placental form (GST-P), were markedly increased. Gomisin A significantly inhibited the increase in number and size of GST-P positive foci, regardless of the promotor. This lignan inhibited the increase in serum bile acid concentration by administration of DCA, but hardly influenced the serum bile acids in the PB-combined group. These results suggest that the inhibitory effect of gomisin A on the promotive action of DCA is based on improving bile acid metabolism, but regarding the action of PB, the effect could not be elucidated from the metabolism of bile acids.
Miyamoto
K
K
Faculty of Pharmaceutical Sciences, Hokuriku University, Kanazawa, Japan.
Hiramatsu
K
K
Ohtaki
Y
Y
Kanitani
M
M
Nomura
M
M
Aburada
M
M
D000818:Animals; D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D001647:Bile Acids and Salts / Q000097:blood* / Q000737:chemistry; D001835:Body Weight / Q000187:drug effects; D002273:Carcinogens / Q000633:toxicity*; D034242:Cyclooctanes; D003840:Deoxycholic Acid / Q000037:antagonists & inhibitors / Q000633:toxicity; D004149:Dioxoles / Q000494:pharmacology*; D007150:Immunohistochemistry; D017705:Lignans; D008114:Liver Neoplasms, Experimental / Q000097:blood* / Q000139:chemically induced / Q000378:metabolism; D008297:Male; D008749:Methyldimethylaminoazobenzene / Q000037:antagonists & inhibitors / Q000633:toxicity*; D009929:Organ Size / Q000187:drug effects; D010634:Phenobarbital / Q000037:antagonists & inhibitors / Q000633:toxicity; D051381:Rats
D016588:Anticarcinogenic Agents; D001647:Bile Acids and Salts; D002273:Carcinogens; D034242:Cyclooctanes; D004149:Dioxoles; D017705:Lignans; D003840:Deoxycholic Acid; D008749:Methyldimethylaminoazobenzene; C033585:schizandrol B; D010634:Phenobarbital