Rodent complementation group 8 (ERCC8) corresponds to Cockayne syndrome complementation group A.
US31 is a UV-sensitive mutant cell line (rodent complementation group 8) derived from a mouse T cell line L5178Y. We analyzed removal kinetics for UV-induced cyclobutane pyrimidine dimers and (6-4) photoproducts in US31 cells using monoclonal antibodies against these photoproducts. While nearly all (6-4) photoproducts were repaired within 6 h after UV-irradiation, more than 70% of cyclobutane pyrimidine dimers remained unrepaired even 24 h after UV-irradiation. These kinetics resembled those of Cockayne syndrome (CS) cells. Since US31 cells had a low efficiency of cell fusion and transfection, which hampered both complementation tests and gene cloning, we constructed fibroblastic complementation group 8 cell line 6L1030 by fusion of US31 cells with X-irradiated normal mouse fibroblastic LTA cells. Complementation tests by cell fusion and transfection using 6L1030 cells revealed that rodent complementation group 8 corresponded to CS complementation group A.
Itoh
T
T
Department of Cell Genetics, Kumamoto University School of Medicine, Japan.
Shiomi
T
T
Shiomi
N
N
Harada
Y
Y
Wakasugi
M
M
Matsunaga
T
T
Nikaido
O
O
Friedberg
E C
EC
Yamaizumi
M
M
D000818:Animals; D002459:Cell Fusion; D002460:Cell Line; D003057:Cockayne Syndrome / Q000235:genetics*; D004247:DNA / Q000528:radiation effects; D004249:DNA Damage; D004260:DNA Repair / Q000235:genetics / Q000502:physiology*; D045643:DNA Repair Enzymes; D004268:DNA-Binding Proteins; D005816:Genetic Complementation Test / Q000379:methods; D006801:Humans; D006822:Hybrid Cells; D007739:L Cells; D007940:Leukemia L5178; D051379:Mice; D011506:Proteins / Q000235:genetics / Q000378:metabolism; D011740:Pyrimidine Dimers / Q000378:metabolism; D014157:Transcription Factors; D014162:Transfection; D014466:Ultraviolet Rays; D014983:Xeroderma Pigmentosum / Q000235:genetics
C490652:Ckn1 protein, mouse; D004268:DNA-Binding Proteins; C095472:ERCC8 protein, human; D011506:Proteins; D011740:Pyrimidine Dimers; D014157:Transcription Factors; C058406:pyrimidine-pyrimidone dimer; D004247:DNA; D045643:DNA Repair Enzymes