title: Quantification and molecular analysis of plasmatic HLA-BCw molecules with a locus B and Cw HLA specific mAb (B1.23.2 mAb).
abstract: Plasmatic HLA class I molecules were analysed quantitatively and qualitatively with a B and Cw locus-specific mAb, B1.23.2. sHLA-BCw were detected among all individuals with a wide range of concentration. The HLA-A29(19) allele was significantly associated with an increased sHLA-BCw mean concentration. Three different isoforms of relative molecular weights of 44, 39 and 36kDa were detected. The 39 kDa class I heavy chain was not detected after immunoprecipitation with mAb B1.23.2 in 17% of tested plasma, but was immunoprecipitated with W6/32 mAb.
authors.affiliation: Laboratoire d'Immunologie, CHRU Dupuytren, Limoges, France.
authors.forename: M
authors.initials: M
authors.lastname: Drouet
authors.forename: L
authors.initials: L
authors.lastname: Aussel
authors.forename: B
authors.initials: B
authors.lastname: Drenou
authors.forename: R
authors.initials: R
authors.lastname: Fauchet
mesh_terms: D000483:Alleles; D000911:Antibodies, Monoclonal / Q000276:immunology; D015153:Blotting, Western; D015234:HLA-A Antigens / Q000235:genetics; D015235:HLA-B Antigens / Q000097:blood* / Q000276:immunology; D015236:HLA-C Antigens / Q000097:blood* / Q000276:immunology; D006801:Humans; D007518:Isoantibodies / Q000276:immunology; D008970:Molecular Weight; D011233:Precipitin Tests; D012995:Solubility
chemical_list: D000911:Antibodies, Monoclonal; D015234:HLA-A Antigens; C035524:HLA-A29 antigen; D015235:HLA-B Antigens; D015236:HLA-C Antigens; D007518:Isoantibodies