title: Survival and histopathologic characteristics of human melanocytic nevi transplanted to athymic (nude) mice.
abstract: Melanocytic nevi (n = 406) covering a range of sizes and gross morphologic features were excised from human donors, sampled for histologic diagnosis, and transplanted to athymic (nude) mice. Ninety percent of these xenografts survived transplantation, of which a subset was irradiated daily with ultraviolet light to promote neoplastic transformation. Over 16 weeks of observation, nearly all grafts histologically showed focal inflammatory cell infiltration and fibrosis, progressing in approximately 30% of grafts to complete regression at final observation. During the inflammatory phase, the nevi often had junctional intraepidermal melanocytic hyperplasia in a lentiginous pattern, with cytologic hypertrophy, dendritic morphology, and hypermelaninization. These changes were evident in approximately 20-30% of nevi where they were absent before transplantation, suggesting that host factors, such as those related to the immune response, had stimulated growth. Graft survival was independent of nevus size and initial histologic diagnosis. No melanomas developed in any of the grafts, either spontaneously or with ultraviolet irradiation. These results indicate that successful transplantation can be achieved in a high proportion of human nevus xenografts and that the majority survive for a period of time that would be sufficient for experimental studies. The host response, however, has effects on intraepidermal melanocytic growth that lead to progressive fibrous replacement of the nevus, introducing significant artifacts that compromise the model. Furthermore, malignant transformation of engrafted melanocytes seems to be a rare event, which would limit studies of neoplastic progression in the transplanted melanocytes. Nonetheless, the intraepidermal melanocytic pattern described here evidently constitutes one pattern of melanocyte growth that could be exploited experimentally for studies of growth and differentiation control in melanocytes.
authors.affiliation: Department of Medicine (Dermatology), University of Utah School of Medicine, Salt Lake City, USA.
authors.forename: L J
authors.initials: LJ
authors.lastname: Meyer
authors.forename: L A
authors.initials: LA
authors.lastname: Schmidt
authors.forename: D E
authors.initials: DE
authors.lastname: Goldgar
authors.forename: M W
authors.initials: MW
authors.lastname: Piepkorn
mesh_terms: D000818:Animals; D016477:Artifacts; D002471:Cell Transformation, Neoplastic / Q000528:radiation effects; D003713:Dendritic Cells / Q000473:pathology; D004195:Disease Models, Animal; D004817:Epidermis / Q000473:pathology; D005355:Fibrosis; D006085:Graft Survival / Q000276:immunology; D006801:Humans; D017495:Hyperpigmentation / Q000473:pathology; D006965:Hyperplasia; D006984:Hypertrophy; D007249:Inflammation; D007911:Lentigo / Q000473:pathology; D008543:Melanins; D008544:Melanocytes / Q000473:pathology; D051379:Mice; D008819:Mice, Nude; D009365:Neoplasm Regression, Spontaneous; D009368:Neoplasm Transplantation / Q000276:immunology; D009508:Nevus, Pigmented / Q000276:immunology / Q000473:pathology*; D012878:Skin Neoplasms / Q000276:immunology / Q000473:pathology*; D014183:Transplantation, Heterologous / Q000276:immunology; D014466:Ultraviolet Rays
chemical_list: D008543:Melanins