Changes of phage T7 nucleoprotein structure at low ionic strength. A Raman spectroscopic study.
To detect changes in DNA and/or protein structures of phage T7 under different ionic strength, Raman spectra of phage T7 have been recorded in solutions of three different NaCl + Tris concentrations. Iterative Jansson-Van Cittert deconvolution, as well as decomposition methods have been used to quantify changes in DNA structure. Significant modifications in ratios of contributions from 675 and 685 per cm vibrations, as well as in the DNA backbone vibrations, characteristic for B-DNA, near 835 per cm frequency have been found. Changes of the base electronic structure were identified in the interval between 1280 and 1400 cm(-1). Estimation of the overall protein structure suggests predominant beta-sheet content.
Gróf
P
P
Institute of Biophysics, Semmelweis Medical University, Budapest, Hungary.
Aslanian
D
D
Rontó
G
G
D017123:Bacteriophage T7 / Q000737:chemistry*; D004247:DNA / Q000737:chemistry; D009690:Nucleic Acid Conformation; D009698:Nucleoproteins / Q000737:chemistry*; D009994:Osmolar Concentration; D017433:Protein Structure, Secondary; D013059:Spectrum Analysis, Raman
D009698:Nucleoproteins; D004247:DNA