Influence of spontaneous hypertension on n-3 delta-6-desaturase activity and fatty acid composition of rat hepatocytes.
The first rate limiting step in the conversion of alpha-linolenic acid is catalyzed by the delta-6-desaturase enzyme. The activity of such an enzyme was studied in order to investigate the n-3 Polyunsaturated Fatty Acid biogenesis during hypertension. Rat isolated hepatocyte n-3 delta-6-desaturase activity was higher in 1 month old Spontaneously Hypertensive Rats -- prehypertensive period-as compared to normotensive Wistar Kyoto rats, whereas there was no significant differences at 12 months -- hypertensive period-. Our data indicate no correlation between the directly measured enzyme activity and the changes in hepatocyte n-3 fatty acid compositions. The loss of hepatocyte n-3 delta-6-desaturase activity in the Spontaneously Hypertensive Rat may be a key factor in the evolution of hypertension related to aging through altering the eicosanoid balance.
Narce
M
M
Unité de Nutrition Cellulaire et Métabolique, Faculté des Sciences Mirande, Université de Bourgogne, Dijon, France.
Asdrubal
P
P
Delachambre
M C
MC
Gresti
J
J
Poisson
J P
JP
D000818:Animals; D004789:Enzyme Activation; D044943:Fatty Acid Desaturases / Q000378:metabolism*; D005227:Fatty Acids / Q000032:analysis*; D006973:Hypertension / Q000378:metabolism*; D050602:Linoleoyl-CoA Desaturase; D008099:Liver / Q000378:metabolism*; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY
D005227:Fatty Acids; D044943:Fatty Acid Desaturases; D050602:Linoleoyl-CoA Desaturase