[Effect of medium polarity and protein microenvironment on indo-1 fluorescence].
Fluorescent properties of indo-1 in protein containing solutions have been investigated. No changes have been defined in indo-1 fluorescent parameters in solutions of such proteins as trypsin, calmodulin, papain, lysozyme. Significant changes of indo-1 fluorescent parameters have been observed in solutions of histones, bovine and human serum albumins. Results obtained suggest that indo-1 binds well with protein sites containing hydrophobic "pockets" and positively charged amino-acid residues such as arginine and lysin. The destruction of the compact structure of serum albumin in 8 M urea leads to shift of indo-1 fluorescence spectrum and it approaches to the spectrum in water. Fluorescence spectra of indo-1 in dioxane prove that the binding of indo-1 by some proteins occurs in hydrophobic microenvironment of protein globule.
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D000818:Animals; D002147:Calmodulin / Q000737:chemistry; D002417:Cattle; D003470:Culture Media; D005456:Fluorescent Dyes; D006657:Histones / Q000737:chemistry; D006801:Humans; D007211:Indoles; D007477:Ions; D009113:Muramidase / Q000737:chemistry; D010206:Papain / Q000737:chemistry; D011506:Proteins / Q000737:chemistry*; D012709:Serum Albumin / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D014357:Trypsin / Q000737:chemistry
D002147:Calmodulin; D003470:Culture Media; D005456:Fluorescent Dyes; D006657:Histones; D007211:Indoles; D007477:Ions; D011506:Proteins; D012709:Serum Albumin; D012710:Serum Albumin, Bovine; D009113:Muramidase; D014357:Trypsin; D010206:Papain; C048960:indo-1