Hyperplasia of the muscularis propria in response to massive intestinal resection in rat.
Adaptation of the muscularis propria to intestinal resection is not well studied. Growing, 150- to 200-g Sprague-Dawley rats were divided into a resected group (R), which had 75% of the jejunoileum removed; a sham-resected group (SR); and a group of untreated controls (C). Food intake and weight gain were documented. Animals were killed on days 0, 10, 20, 30, or 40 (n = 14 animals for each day in each group). Small-intestinal length and jejunal circumference, wet weight/cm, thickness of the muscle layers, muscle morphometry, mg protein, and micrograms DNA per cm of the muscularis propria were measured. R rats had an initially greater food intake than SR or C animals, but the rate of weight gain was not different between groups. There was no significant increase in length of the residual intestine in R animals during the postoperative adaptive period, but circumference was significantly increased in R compared with SR or C animals. Wet weight/cm, mg protein, and micrograms DNA per cm of the muscularis propria were significantly greater in R compared with SR or C animals, but there was no change in thickness of the smooth-muscle layers in R versus SR or C rats. The number of cross-sectioned circular smooth-muscle cells/mm2 and the ratio of mg protein/micrograms DNA/cm length of muscularis propria were not different in R compared with SR or C animals. Intestinal resection resulted in a significant increase in circumference but not length of the residual intestine. A significant increase in the mass/cm, with no change in the number of cross-sectioned circular smooth-muscle cells/mm2 or in mg protein/micrograms DNA/cm of the muscularis propria, indicates that the adaptive response of muscularis propria is characterized by hyperplasia. This response potentially confers benefit to the organ and animal as a whole as a mechanism that permits structural and functional adaptation of the muscularis propria to resection.
Scott
R B
RB
Gastroenterology Research Group, University of Calgary, Alberta, Canada.
Sheehan
A
A
Chin
B C
BC
Tan
D T
DT
D000818:Animals; D004247:DNA / Q000378:metabolism; D004435:Eating; D006965:Hyperplasia; D007082:Ileum / Q000601:surgery; D007413:Intestinal Mucosa / Q000473:pathology; D007421:Intestine, Small / Q000473:pathology* / Q000601:surgery*; D007583:Jejunum / Q000473:pathology / Q000601:surgery; D009132:Muscles / Q000473:pathology*; D009929:Organ Size; D011506:Proteins / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
D011506:Proteins; D004247:DNA