Pancreatic islet mitochondrial glycerophosphate dehydrogenase deficiency in two animal models of non-insulin-dependent diabetes mellitus.
Western blotting of pancreatic islet extracts from either hereditarily diabetic Goto-Kakizaki rats (GK rats) or animals injected with streptozotocin during the neonatal period (STZ rats) demonstrated a pronounced decrease of immunoreactive mitochondrial glycerophosphate dehydrogenase (m-GDH), when compared to results obtained in islets from control rats. By contrast, the islet glucokinase protein content was either unaffected (GK rats) or much less severely decreased than that of m-GDH (STZ rats). These findings indicate that the impaired activity of m-GDH previously documented in islet homogenates from diabetic rats coincides with a decreased content of this enzyme in the endocrine pancreas.
Fabregat
M E
ME
Endocrinology Unit, Hospital Clinic, Barcelona University, Spain.
Novials
A
A
Giroix
M H
MH
Sener
A
A
Gomis
R
R
Malaisse
W J
WJ
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D015153:Blotting, Western; D003921:Diabetes Mellitus, Experimental / Q000201:enzymology*; D003924:Diabetes Mellitus, Type 2 / Q000201:enzymology* / Q000235:genetics; D005941:Glucokinase / Q000378:metabolism; D005993:Glycerolphosphate Dehydrogenase / Q000172:deficiency* / Q000235:genetics / Q000378:metabolism*; D007328:Insulin / Q000097:blood; D007515:Islets of Langerhans / Q000201:enzymology*; D008928:Mitochondria / Q000201:enzymology*; D051381:Rats; D011922:Rats, Mutant Strains; D017208:Rats, Wistar; D012016:Reference Values
D001786:Blood Glucose; D007328:Insulin; D005993:Glycerolphosphate Dehydrogenase; D005941:Glucokinase