title: Cloning of a cellulase gene from the rumen anaerobe Fibrobacter succinogenes SD35 and partial characterization of the gene product.
abstract: A gene encoding an enzyme which degrades cellulose (end-1) was isolated from a library of Fibrobacter succinogenes SD35 DNA fragments and expressed in pUC18. The product of end-1 showed significant activity against carboxymethylcellulose but relatively minor activity against lichenan, xylan and avicel. The nucleotide sequence indicated a product of 388 amino acids with a molecular mass of 50.2 kDa. This was in agreement with the molecular size estimated by gel electrophoresis. No significant DNA sequence similarity was identified with any published endoglucanase.
authors.affiliation: Department of Molecular and Cell Biology, Marischal College, University of Aberdeen, Scotland, UK.
authors.forename: N
authors.initials: N
authors.lastname: Ozcan
authors.forename: C
authors.initials: C
authors.lastname: Cunningham
authors.forename: W J
authors.initials: WJ
authors.lastname: Harris
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001439:Bacteroides / Q000201:enzymology* / Q000235:genetics*; D002266:Carboxymethylcellulose Sodium; D002480:Cellulase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D015870:Gene Expression; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D008970:Molecular Weight; D012417:Rumen / Q000382:microbiology; D013379:Substrate Specificity
chemical_list: D004269:DNA, Bacterial; D002480:Cellulase; D002266:Carboxymethylcellulose Sodium