Developmental expression pattern of the Caenorhabditis elegans homologue of the Drosophila suppressor of forked gene.
We cloned and sequenced the cDNA which encodes the Caenorhabditis elegans homologue (suf-1) of the Drosophila suppressor of forked (su(f)) gene product. The amino acid sequence deduced from the suf-1 cDNA shares extensive similarity with the su(f) and the human 77K subunit of the CstF, including the proline residues near the carboxy-terminus. Developmental Northern blot analysis showed that a 2.6-kb suf-1 transcript was expressed constitutively from L1 to young adult stages, suggesting it has a housekeeping function in vivo. Furthermore, this idea is consistent with the recent proposition that su(f) homologues play an essential role as a subunit of the cleavage stimulation factor (CstF) during polyadenylation of mRNA precursors.
Tanaka
Y
Y
Graduate School of Science and Technology, Kobe University, Japan.
Ohta
A
A
Matsuo
M
M
Sakamoto
H
H
D000595:Amino Acid Sequence; D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics* / Q000254:growth & development; D018076:DNA, Complementary / Q000235:genetics; D029721:Drosophila Proteins; D017238:Genes, Helminth; D016153:Genes, Suppressor; D015801:Helminth Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D009687:Nuclear Proteins; D011506:Proteins / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D029721:Drosophila Proteins; D015801:Helminth Proteins; D009687:Nuclear Proteins; D011506:Proteins; D012333:RNA, Messenger; C079745:su(f) protein, Drosophila