title: Crystal structure of a yeast TFIIA/TBP/DNA complex.
abstract: The X-ray crystal structure of the transcription factor IIA (TFIIA) in complex with the TATA-box-binding protein (TBP) and TATA-element DNA is presented at 2.5 A resolution. TFIIA is composed of a beta-barrel and a four-helix bundle motif that together have a boot-like appearance. The beta-barrel extends the TBP beta-sheet and bridges over the DNA major groove immediately upstream of the TATA box. The four-helix bundle contributes substantially to the surface of the complex available for interaction with additional transcription factors.
authors.affiliation: Institut für Molekularbiologie und Biophysik, ETH-Hönggerberg, Zürich, Switzerland.
authors.forename: S
authors.initials: S
authors.lastname: Tan
authors.forename: Y
authors.initials: Y
authors.lastname: Hunziker
authors.forename: D F
authors.initials: DF
authors.lastname: Sargent
authors.forename: T J
authors.initials: TJ
authors.lastname: Richmond
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D018360:Crystallography, X-Ray; D003574:Cytochrome c Group / Q000235:genetics; D045304:Cytochromes c; D004271:DNA, Fungal / Q000737:chemistry*; D004268:DNA-Binding Proteins / Q000737:chemistry*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000737:chemistry; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins; D016385:TATA Box; D035181:TATA-Box Binding Protein; D035561:Transcription Factor TFIIA; D014157:Transcription Factors / Q000737:chemistry*; D014158:Transcription, Genetic
chemical_list: C014059:CYC1 protein, S cerevisiae; D003574:Cytochrome c Group; D004271:DNA, Fungal; D004268:DNA-Binding Proteins; D011994:Recombinant Proteins; D029701:Saccharomyces cerevisiae Proteins; D035181:TATA-Box Binding Protein; D035561:Transcription Factor TFIIA; D014157:Transcription Factors; D045304:Cytochromes c