Calculation of translational friction and intrinsic viscosity. II. Application to globular proteins.
The translational friction coefficients and intrinsic viscosities of four proteins (ribonuclease A, lysozyme, myoglobin, and chymotrypsinogen A) are calculated using atomic-level structural details. Inclusion of a 0.9-A-thick hydration shell allows calculated results for both hydrodynamic properties of each protein to reproduce experimental data. The use of detailed protein structures is made possible by relating translational friction and intrinsic viscosity to capacitance and polarizability, which can be calculated easily. The 0.9-A hydration shell corresponds to a hydration level of 0.3-0.4 g water/g protein. Hydration levels within this narrow range are also found by a number of other techniques such as nuclear magnetic resonance spectroscopy, infrared spectroscopy, calorimetry, and computer simulation. The use of detailed protein structures in predicting hydrodynamic properties thus allows hydrodynamic measurement to join the other techniques in leading to a unified picture of protein hydration. In contrast, earlier interpretations of hydrodynamic data based on modeling proteins as ellipsoids gave hydration levels that varied widely from protein to protein and thus challenged the existence of a unified picture of protein hydration.
Zhou
X Z
XZ
Department of Biochemistry, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong. bchxzhou@uxmail.ust.hk
D000465:Algorithms; D002919:Chymotrypsinogen / Q000737:chemistry; D008433:Mathematics; D008961:Models, Structural; D008962:Models, Theoretical; D009113:Muramidase / Q000737:chemistry; D009211:Myoglobin / Q000737:chemistry; D055585:Physical Phenomena; D010825:Physics; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry*; D012259:Ribonuclease, Pancreatic / Q000737:chemistry; D012996:Solutions; D014783:Viscosity
D009211:Myoglobin; D011506:Proteins; D012996:Solutions; D002919:Chymotrypsinogen; D012259:Ribonuclease, Pancreatic; D009113:Muramidase