Streptozotocin, an inducer of NAD+ decrease, attenuates M-potassium current inhibition by ATP, bradykinin, angiotensin II, endothelin 1 and acetylcholine in NG108-15 cells.
The M-potassium current was inhibited by bath application of 100 micron ATP, 10 nM bradykinin, 100 nM angiotensin II and 100 nM endothelin 1 as well as by 10 micron acetylcholine in an m1-muscarinic acetylcholine receptor-transformed NG108-15 cell line. The inhibition of M-current was attenuated in cells pretreated with 5 mM streptozotocin for 5-15 h and restored by simultaneous incubation with 5 mM nicotinamide. The results suggest that signal transduction from these five different receptors to M channels shares a common pathway which is susceptible to a streptozotocin-induced decrease in cellular NAD+ content.
Higashida
H
H
Department of Biophysics, Kanazawa University School of Medicine, Japan.
Egorova
A
A
Hoshi
N
N
Noda
M
M
D000109:Acetylcholine / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000494:pharmacology; D000804:Angiotensin II / Q000494:pharmacology; D000818:Animals; D001920:Bradykinin / Q000494:pharmacology; D016232:Endothelins / Q000494:pharmacology; D005910:Glioma; D006822:Hybrid Cells; D051379:Mice; D009243:NAD / Q000378:metabolism*; D009447:Neuroblastoma; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism*; D011188:Potassium / Q000378:metabolism*; D051381:Rats; D015398:Signal Transduction / Q000187:drug effects*; D013311:Streptozocin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D016232:Endothelins; D009243:NAD; D000804:Angiotensin II; D013311:Streptozocin; D000255:Adenosine Triphosphate; D000109:Acetylcholine; D011188:Potassium; D001920:Bradykinin