Hematopoietic progenitors and interleukin-3-dependent cell lines synthesize histamine in response to calcium ionophore.
The calcium ionophore A23187 promotes histamine synthesis in murine bone marrow cells by increasing the expression of mRNA encoding histidine decarboxylase (HDC), the histamine-forming enzyme. The cells responsible for this biological activity copurify with hematopoietic progenitors in terms of density, light scatter characteristics, and rhodamine retention, similar to interleukin (IL) 3-induced histamine-producing cells. Yet, the effect of calcium ionophore is not mediated by IL-3. The most purified rhodamine-bright bone marrow subset contains 80% cells that respond to calcium ionophore by increased HDC mRNA expression. This high frequency makes the involvement of one particular progenitor subset in histamine synthesis unlikely. The finding that all IL-3-dependent cell lines tested so far exhibit increased histamine production and HDC mRNA expression in response to calcium influx lends further support to this notion. Cell lines requiring other growth factors or proliferating spontaneously lack this ability. Finally, it should be noted that IL-3-dependent cell lines do not produce histamine in response to their growth factor. It might, therefore, be suggested that the pathway transducing the signal for increased histamine synthesis after IL-3 receptor binding in normal hematopoietic progenitors is modified in these cell lines.
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CNRS URA 1461, Université René Descartes-Paris V, Hôpital Necker, France.
Arnould
A
A
Lemoine
F M
FM
Machavoine
F
F
Ziltener
H
H
Schneider
E
E
D000818:Animals; D001483:Base Sequence; D001692:Biological Transport; D001853:Bone Marrow / Q000187:drug effects; D001854:Bone Marrow Cells; D000001:Calcimycin / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism*; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002478:Cells, Cultured; D004790:Enzyme Induction / Q000187:drug effects; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D006412:Hematopoietic Stem Cells / Q000187:drug effects*; D006632:Histamine / Q000096:biosynthesis*; D006640:Histidine Decarboxylase / Q000096:biosynthesis / Q000235:genetics; D007377:Interleukin-3 / Q000494:pharmacology*; D007476:Ionophores / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000096:biosynthesis; D011994:Recombinant Proteins / Q000494:pharmacology
D007377:Interleukin-3; D007476:Ionophores; D012333:RNA, Messenger; D011994:Recombinant Proteins; D000001:Calcimycin; D006632:Histamine; D006640:Histidine Decarboxylase; D002118:Calcium