MHC bias of Mls-1 recognition is not influenced by thymic positive selection.
In contrast to T cell recognition of conventional peptide/MHC, T cell recognition of superantigen is not MHC-restricted. However, an influence of MHC polymorphism on specificity is consistent with accumulating data suggesting a TCR/MHC interaction during T cell recognition of superantigen. We have previously shown that T cells from V beta 8.1 beta-chain transgenic mice show an unexpected bias against recognition of Mls-1 presented by H-2d spleen cells. In the current studies we have examined whether thymic positive selection in H-2d mice, which selects T cells that see conventional antigen preferentially in the context of H-2d, is able to overcome the strong bias against recognition of Mls-1/H-2d. The data show that transgenic T cells from both H-2d and H-2k mice have comparable reactivity. The failure of thymic positive selection to overcome the bias against Mls-1/H-2d suggests that the orientation of the putative TCR/MHC interaction during recognition of Mls-1 is not the same as during recognition of conventional peptide/MHC.
Nguyen
P
P
Department of Immunology, St. Jude Children's Research Hospital, Memphis, Tennessee 38105, USA.
Woodland
D L
DL
Blackman
M A
MA
D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology; D004768:Enterotoxins / Q000276:immunology; D006183:H-2 Antigens / Q000276:immunology*; D006239:Haplotypes; D006825:Hybridomas; D008285:Major Histocompatibility Complex; D051379:Mice; D008822:Mice, Transgenic; D016747:Minor Lymphocyte Stimulatory Antigens / Q000276:immunology*; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000276:immunology*; D018089:Superantigens / Q000276:immunology; D013950:Thymus Gland / Q000276:immunology
D004768:Enterotoxins; D006183:H-2 Antigens; D016747:Minor Lymphocyte Stimulatory Antigens; D016693:Receptors, Antigen, T-Cell, alpha-beta; D018089:Superantigens; C031818:enterotoxin B, staphylococcal