Characterization of 2 aquareovirus proteins.
The protein products of aquareovirus genome segments 10 and 11 were investigated. The genome segment 10 encodes the major outer capsid protein (VP7) of aquareovirus. The VP7 protein has a molecular weight of 35 kDa and was found to be glycosylated. The electrophoretic mobility of VP7 in SDS-PAGE was unaltered when untreated and deglycosylated proteins were compared, indicating the presence of short oligosaccharide chains. Selective binding of lectins with well-defined sugar specificities suggest that most of the short N-linked carbohydrate chains of VP7 contain galactose residues. Genome segment 11 encoded 2 non-structural proteins (NS29 and NS15). Partial protease mapping indicated that the NS29 and NS15 proteins are derived from initiation of protein synthesis at 2 distinct sites.
Samal
S K
SK
College of Veterinary Medicine, Maryland Agricultural Experiment Station, University of Maryland, College Park 20742, USA.
Subramanian
K
K
Lupiani
B
B
D000818:Animals; D000956:Antigens, Viral; D001492:Bass / Q000821:virology*; D002213:Capsid / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism; D036022:Capsid Proteins; D002460:Cell Line; D004625:Embryo, Nonmammalian; D016679:Genome, Viral; D006031:Glycosylation; D014176:Protein Biosynthesis; D011499:Protein Processing, Post-Translational; D012330:RNA, Double-Stranded / Q000302:isolation & purification / Q000378:metabolism; D012367:RNA, Viral / Q000302:isolation & purification / Q000378:metabolism; D012087:Reoviridae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D012474:Salmon
D000956:Antigens, Viral; D036022:Capsid Proteins; D012330:RNA, Double-Stranded; D012367:RNA, Viral; C050779:VP7 protein, Rotavirus