Expression of Rab9 protein in Escherichia coli: purification and isoprenylation in vitro.
Riederer
M A
MA
Department of Biochemistry, Stanford University School of Medicine, California 94305, USA.
Soldati
T
T
Dirac-Svejstrup
A B
AB
Pfeffer
S R
SR
D000818:Animals; D000906:Antibodies; D002850:Chromatography, Gel / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000379:methods; D003001:Cloning, Molecular / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D004926:Escherichia coli; D020558:GTP Phosphohydrolases / Q000096:biosynthesis* / Q000302:isolation & purification*; D015151:Immunoblotting; D007202:Indicators and Reagents; D051379:Mice / Q000276:immunology; D011106:Polyisoprenyl Phosphates / Q000378:metabolism; D017368:Protein Prenylation; D011817:Rabbits / Q000276:immunology; D011865:Radioisotope Dilution Technique; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000302:isolation & purification; D014316:Tritium; D020691:rab GTP-Binding Proteins
D000906:Antibodies; D007202:Indicators and Reagents; D011106:Polyisoprenyl Phosphates; D011994:Recombinant Proteins; D014316:Tritium; D020558:GTP Phosphohydrolases; D020691:rab GTP-Binding Proteins; C002963:geranylgeranyl pyrophosphate