Infections with various types of organisms stimulate transcription from a short promoter fragment of the potato gst1 gene.
By histochemical GUS staining, we demonstrate that transcription from a short promoter fragment of the potato gst1 gene is locally induced after infection of a host plant with various types of pathogenic or symbiotic organisms. This regulatory unit is not active in noninfected tissues, except root apices and senescing leaves. Measuring the expression of a fusion between the promoter fragment and the gus gene in transgenic plants, therefore, allows comparison of the induction of defense reactions in different types of plant-microbe interactions, in one and the same plant.
Strittmatter
G
G
Max-Planck-Institut für Züchtungsforschung, Abteilung Biochemie, Köln, Germany.
Gheysen
G
G
Gianinazzi-Pearson
V
V
Hahn
K
K
Niebel
A
A
Rohde
W
W
Tacke
E
E
D000818:Animals; D005658:Fungi / Q000472:pathogenicity; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000235:genetics*; D005982:Glutathione Transferase / Q000096:biosynthesis / Q000235:genetics*; D009348:Nematoda / Q000472:pathogenicity; D010935:Plant Diseases / Q000235:genetics*; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics*; D017800:Potyvirus / Q000472:pathogenicity; D011401:Promoter Regions, Genetic; D011198:Solanum tuberosum / Q000201:enzymology / Q000235:genetics*; D014158:Transcription, Genetic
D010940:Plant Proteins; D005982:Glutathione Transferase