title: The kinetics of induction of Hox1.6 and C-jun mRNA during three different ways of inducing differentiation in teratocarcinoma F9 cells.
abstract: Changes in Hox1.6 and c-jun gene expression were examined upon F9 cell differentiation that was induced by three independent methods: a drug treatment with retinoic acid (RA), that with sodium butyrate (NaB), and a genetic approach using the ts mutant. To obtain further information on the mechanism of teratocarcinoma cell differentiation we have examined the kinetics of the induction of Hox1.6 and c-jun mRNA whose gene products have been demonstrated to have specific roles in gene regulation. Expression of Hox1.6 mRNA was induced more rapidly than c-jun mRNA by all the above three inducing methods. Furthermore, protein synthesis was not required for the induction of Hox1.6 mRNA as well as of c-jun mRNA synthesis in all three methods. The data suggested that the transcriptional increase in the Hox1.6 mRNA was a primary response and could play an important role in F9 cell differentiation.
authors.affiliation: 2nd Department of Oral Surgery, Dental Faculty, Osaka University, Japan.
authors.forename: S A
authors.initials: SA
authors.lastname: Iwai
authors.forename: Y
authors.initials: Y
authors.lastname: Nishina
authors.forename: M
authors.initials: M
authors.lastname: Kosaka
authors.forename: T
authors.initials: T
authors.lastname: Sumi
authors.forename: T
authors.initials: T
authors.lastname: Doi
authors.forename: M
authors.initials: M
authors.lastname: Sakuda
authors.forename: Y
authors.initials: Y
authors.lastname: Nishimune
mesh_terms: D002087:Butyrates / Q000494:pharmacology*; D020148:Butyric Acid; D002454:Cell Differentiation / Q000235:genetics; D003513:Cycloheximide / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects*; D018398:Homeodomain Proteins / Q000235:genetics*; D006801:Humans; D007700:Kinetics; D009154:Mutation; D016755:Proto-Oncogene Proteins c-jun / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism; D018243:Teratocarcinoma; D014157:Transcription Factors / Q000235:genetics*; D014212:Tretinoin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured; D000099261:Homeobox A1 Protein
chemical_list: D002087:Butyrates; D020148:Butyric Acid; D003513:Cycloheximide; D018398:Homeodomain Proteins; D016755:Proto-Oncogene Proteins c-jun; D012333:RNA, Messenger; D014157:Transcription Factors; D014212:Tretinoin; D000099261:Homeobox A1 Protein