title: An integrated map of the genome of the tubercle bacillus, Mycobacterium tuberculosis H37Rv, and comparison with Mycobacterium leprae.
abstract: An integrated map of the genome of the tubercle bacillus, Mycobacterium tuberculosis, was constructed by using a twin-pronged approach. Pulsed-field gel electrophoretic analysis enabled cleavage sites for Asn I and Dra I to be positioned on the 4.4-Mb circular chromosome, while, in parallel, clones from two cosmid libraries were ordered into contigs by means of fingerprinting and hybridization mapping. The resultant contig map was readily correlated with the physical map of the genome via the landmarked restriction sites. Over 165 genes and markers were localized on the integrated map, thus enabling comparisons with the leprosy bacillus, Mycobacterium leprae, to be undertaken. Mycobacterial genomes appear to have evolved as mosaic structures since extended segments with conserved gene order and organization are interspersed with different flanking regions. Repetitive sequences and insertion elements are highly abundant in M. tuberculosis, but the distribution of IS6110 is apparently nonrandom.
authors.affiliation: Unite de Genetique Moleculaire Bacterienne, Institut Pasteur, Paris, France.
authors.forename: W J
authors.initials: WJ
authors.lastname: Philipp
authors.forename: S
authors.initials: S
authors.lastname: Poulet
authors.forename: K
authors.initials: K
authors.lastname: Eiglmeier
authors.forename: L
authors.initials: L
authors.lastname: Pascopella
authors.forename: V
authors.initials: V
authors.lastname: Balasubramanian
authors.forename: B
authors.initials: B
authors.lastname: Heym
authors.forename: S
authors.initials: S
authors.lastname: Bergh
authors.forename: B R
authors.initials: BR
authors.lastname: Bloom
authors.forename: W R
authors.initials: WR
authors.lastname: Jacobs
authors.forename: S T
authors.initials: ST
authors.lastname: Cole
mesh_terms: D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D003360:Cosmids; D004251:DNA Transposable Elements; D015252:Deoxyribonucleases, Type II Site-Specific; D015723:Gene Library; D005819:Genetic Markers; D016680:Genome, Bacterial; D009166:Mycobacterium leprae / Q000235:genetics*; D009169:Mycobacterium tuberculosis / Q000235:genetics*; D015183:Restriction Mapping; D013045:Species Specificity
chemical_list: D004251:DNA Transposable Elements; D005819:Genetic Markers; C056778:endodeoxyribonuclease AsnI; D015252:Deoxyribonucleases, Type II Site-Specific; C035594:TTTAAA -specific type II deoxyribonucleases