Two-dimensional chemical shift/heteronuclear dipolar coupling spectra obtained with polarization inversion spin exchange at the magic angle and magic-angle sample spinning (PISEMAMAS).
High-resolution two-dimensional 15N chemical shift/1H-15N dipolar coupling polarization inversion spin exchange at the magic angle (PISEMA) spectra of a polycrystalline sample of 15N-acetylvaline were obtained with and without magic-angle sample spinning. These spectra demonstrate the advantages of the PISEMA experiment over conventional approaches to separated local-field spectroscopy, especially the high resolution in the dipolar dimension where the spinning sidebands have uniformly narrow linewidths.
Ramamoorthy
A
A
Department of Chemistry, University of Pennsylvania, Philadelphia 19104, USA.
Opella
S J
SJ
D003460:Crystallization; D004578:Electron Spin Resonance Spectroscopy; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D014633:Valine / Q000031:analogs & derivatives* / Q000737:chemistry
D014633:Valine; C055589:N-acetylvaline