Expression of the rat MHC class I gene in the human B lymphoblastoid cell line Hmy 2 CIR.
Rat MHC class I cDNA (e.g. RT1.A1) can be expressed optimally as a heterodimer consisting of the rat heavy chain and the human beta2-microglobulin in the human B lymphoblastoid cell line Hmy 2 CIR, which offers several advantages. Gene expression was detected by flow cytometry using anti-human beta2-microglobulin and anti-rat heavy chain antibodies.
Salgar
S K
SK
Department of Pathology, University of Pittsburgh, School of Medicine, PA 15261, USA.
Kunz
H W
HW
Gill
T J
TJ
D000818:Animals; D001402:B-Lymphocytes / Q000378:metabolism*; D002460:Cell Line; D003001:Cloning, Molecular; D015870:Gene Expression; D005805:Genes, MHC Class I / Q000235:genetics*; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics / Q000378:metabolism; D051381:Rats; D014162:Transfection; D001613:beta 2-Microglobulin / Q000235:genetics / Q000378:metabolism
D007143:Immunoglobulin Heavy Chains; D001613:beta 2-Microglobulin