Mxi1 tumor suppressor gene is not mutated in primary pancreatic adenocarcinoma.
Amplification of the c-myc oncogene occurs in a variety of solid tumors, including pancreatic adenocarcinomas. The MXI1 gene, located at 10q24-q25, may serve to negatively regulate c-myc oncogene activity, and potentially has tumor suppressor function. As such, altered MXI1 function might contribute to tumorigenesis. We examined 40 primary human pancreatic adenocarcinomas for MXI1 mutations. Single-strand conformation variant analysis and direct sequencing of the variants revealed a MXI1 polymorphism in 1 of 40 tumors. No MXI1 mutations were identified. Southern blot analyses did not reveal any gross rearrangements of MXI1. These results suggest that MXI1 is unlikely to play a role in human pancreatic adenocarcinoma tumorigenesis.
Bartsch
D
D
Department of Surgery, Washington University School of Medicine, St. Louis, MO, USA.
Peiffer
S L
SL
Kaleem
Z
Z
Wells
S A
SA
Goodfellow
P J
PJ
D000230:Adenocarcinoma / Q000235:genetics*; D051792:Basic Helix-Loop-Helix Proteins; D015139:Blotting, Southern; D018270:Carcinoma, Ductal, Breast / Q000235:genetics*; D004273:DNA, Neoplasm / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D016147:Genes, Tumor Suppressor; D016259:Genes, myc; D006801:Humans; D009154:Mutation; D010190:Pancreatic Neoplasms / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*; D025521:Tumor Suppressor Proteins
D051792:Basic Helix-Loop-Helix Proteins; D004273:DNA, Neoplasm; D004268:DNA-Binding Proteins; D014157:Transcription Factors; D025521:Tumor Suppressor Proteins; C079440:MXI1 protein, human