Expression and functional characterization of the cardiac L-type calcium channel carrying a skeletal muscle DHP-receptor mutation causing hypokalaemic periodic paralysis.
A histidine substitution for the outermost arginine in II/S4 of the alpha1 subunit of the human skeletal muscle dihydropyridine (DHP) receptor has been reported to cause hypokalaemic periodic paralysis (HypoPP). This mutation shifts the voltage dependence of L-type Ca curent inactivation in myotubes from HypoPP patients by -40 mV without affecting activation. Based on the strong homology of II/S4 in cardiac and skeletal muscle alpha1, we introduced the corresponding mutation into the rabbit cardiac alpha1 subunit (R650H). Wild type (WT) and mutant constructs were transiently transfected in HEK cells together with beta and alpha2delta subunits and Ca and Ba currents were studied using the whole-cell patch-clamp technique. In contrast to the results obtained from human myotubes, R650H produced a small (-5 mV) but significant shift of both the steady-state activation and inactivation curves. When external pH was increased from 7.4 to 8.4 in order to favour deprotonization of H650, the only difference between WT and mutant channels was a slightly reduced steepness of the inactivation curve. Additional cotransfection of the gamma subunit which is only found in skeletal but not in heart muscle, shifted the inactivation curves of both WT and R650H by -20 mV. We conclude that R650 plays a different role in voltage-dependent gating of the cardiac L-type Ca channel than the corresponding residue in the human skeletal muscle L-type channel, since a distinct and selective effect on the midpoint voltage of steady-state inactivation could not be found for R650H.
Lerche
H
H
Abteilung für Angewandte Physiologie, Universität Ulm, D-89069 Ulm, Germany.
Klugbauer
N
N
Lehmann-Horn
F
F
Hofmann
F
F
Melzer
W
W
D000818:Animals; D001120:Arginine / Q000235:genetics; D001464:Barium / Q000378:metabolism; D001483:Base Sequence; D015220:Calcium Channels / Q000235:genetics* / Q000502:physiology*; D020746:Calcium Channels, L-Type; D006639:Histidine / Q000235:genetics; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007008:Hypokalemia / Q000209:etiology*; D007473:Ion Channels; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000737:chemistry*; D009154:Mutation; D009206:Myocardium / Q000737:chemistry; D010245:Paralyses, Familial Periodic / Q000209:etiology*; D011817:Rabbits
D015220:Calcium Channels; D020746:Calcium Channels, L-Type; D007473:Ion Channels; D001464:Barium; D006639:Histidine; D001120:Arginine