An updated two-dimensional gel database of rat liver proteins useful in gene regulation and drug effect studies.
We have improved upon the reference two-dimensional (2-D) electrophoretic map of rat liver proteins originally published in 1991 (N. L. Anderson et al., Electrophoresis 1991, 12, 907-930). A total of 53 proteins (102 spots) are now identified, many by microsequencing. In most cases, spots cut from wet, Coomassie Blue stained 2-D gels were submitted to internal tryptic digestion [2], and individual peptides, separated by high-performance liquid chromatography (HPLC), were sequenced using a Perkin-Elmer 477A sequenator. Additional spots were identified using specific antibodies.
Anderson
N L
NL
Large Scale Biology Corporation, Rockville, MD 20850-3338, USA. leigh@lsbc,com
Esquer-Blasco
R
R
Hofmann
J P
JP
Meheus
L
L
Raymackers
J
J
Steiner
S
S
Witzmann
F
F
Anderson
N G
NG
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D015180:Electrophoresis, Gel, Two-Dimensional; D006863:Hydrogen-Ion Concentration; D007256:Information Systems; D007526:Isoelectric Point; D008099:Liver / Q000737:chemistry*; D051379:Mice; D008970:Molecular Weight; D010446:Peptide Fragments / Q000737:chemistry; D011506:Proteins / Q000032:analysis / Q000737:chemistry*; D051381:Rats; D017421:Sequence Analysis; D014357:Trypsin
D010446:Peptide Fragments; D011506:Proteins; D014357:Trypsin