Lipid mediators in the rat retina: light exposure and trauma elicit leukotriene B4 release in vitro.
Light exposure not only elicits a visual response but may also alter functional and structural characteristics of the retina. Furthermore, light exposure can lead to reversible or irreversible lesions of photoreceptors and pigment epithelium. Previous studies in our laboratory have shown that light liberates arachidonic acid from retinal membrane phospholipids mainly by activating the phospholipase A2. In this study we show that light and trauma elicit the synthesis of leukotriene B4 in the isolated rat retina in vitro. Male albino rats were dark adapted for 36 h, isolated retinae were taken, incubated and exposed a) either to darkness or to 5,000 lux of cool white fluorescent light for 5, 10 or 15 min at 37 degrees C, b) either to darkness or to 5,000 lux of cool white fluorescent light for 15 min at 0 degrees C or c) either to darkness or to 5,000 lux of cool white fluorescent light for 15 min at 37 degrees C with a 5-lipoxygenase inhibitor (zileuton). Eicosanoids were extracted and leukotriene B4 levels were determined by radioimmunoassay. Removal of retinae and incubation in darkness caused a significant rise in leukotriene B4 levels with increasing incubation time. This rise was further augmented significantly after light exposure. The leukotriene B4 levels obtained when incubating the retinae either at 0 degree C or with the lipoxygenase inhibitor zileuton as well as the high specificity of the radioimmunoassay indicate that the light- and trauma-elicited synthesis of leukotriene B4 is mediated by activating the 5-lipoxygenase. Leukotriene B4 may be involved, at least in part, in the pathogenesis of retinal diseases including light damage. Curr. Eye Res. 14: 1001-1008, 1995.
Reinboth
J J
JJ
Department of Ophthalmology, University Hospital Zürich, Switzerland.
Gautschi
K
K
Clausen
M
M
Remé
C E
CE
D000818:Animals; D001094:Arachidonate 5-Lipoxygenase / Q000502:physiology; D015777:Eicosanoids / Q000032:analysis; D004789:Enzyme Activation; D006918:Hydroxyurea / Q000031:analogs & derivatives / Q000494:pharmacology; D007975:Leukotriene B4 / Q000032:analysis* / Q000096:biosynthesis* / Q000502:physiology; D008027:Light / Q000009:adverse effects*; D050356:Lipid Metabolism; D016859:Lipoxygenase Inhibitors / Q000494:pharmacology; D008297:Male; D011833:Radiation Injuries, Experimental / Q000209:etiology / Q000378:metabolism*; D011863:Radioimmunoassay; D051381:Rats; D012160:Retina / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects*; D012243:Rhodopsin / Q000032:analysis
D015777:Eicosanoids; D016859:Lipoxygenase Inhibitors; D007975:Leukotriene B4; D012243:Rhodopsin; D001094:Arachidonate 5-Lipoxygenase; C063449:zileuton; D006918:Hydroxyurea