Cloning of a cluster of chitinase genes from Aeromonas sp. No. 10S-24.
A gene encoding chitinases from Aeromonas sp. No. 10S-24 was cloned into Escherichia coli DH5 alpha using pUC19, and its nucleotides were sequenced. The chitinase gene was clustered in ORFs (open reading frame) 1 to 4, in a 8-kb fragment of DNA. ORF-1 consisted of 1608 bp encoding 535 amino acid residues, and ORF-2 consisted of 1425 bp encoding 474 amino acid residues. ORF-3 was 1617 bp long and encodes a protein consisting of 538 amino acids. ORF-4 encodes 287 amino acids of the N-terminal region. The amino acid sequences of ORF-1 and ORF-3 share sequence homology with chitinase D from Bacillus circulans, and chitinase A and B from Streptomyces lividans. The amino acid sequence of ORF-2 shared sequence homology with chitinase II from Aeromonas sp. No. 10S-24, and chitinase from Saccharopolyspora erythraea. A region of the sequence starting from Ala-28 of the amino acid sequence of ORF-3 coincided with the N-terminal amino acid sequence of chitinase III from Aeromonas sp. No. 10S-24.
Shiro
M
M
Department of Applied Biological Chemistry, College of Agriculture, University of Osaka Prefecture, Sakai, Japan.
Ueda
M
M
Kawaguchi
T
T
Arai
M
M
D000333:Aeromonas / Q000201:enzymology* / Q000235:genetics*; D000595:Amino Acid Sequence; D001407:Bacillus / Q000201:enzymology / Q000235:genetics; D001483:Base Sequence; D002688:Chitinases / Q000737:chemistry / Q000235:genetics*; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D015394:Molecular Structure; D005810:Multigene Family; D016366:Open Reading Frames; D016930:Saccharopolyspora / Q000201:enzymology / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D013302:Streptomyces / Q000201:enzymology / Q000235:genetics
D004269:DNA, Bacterial; D002688:Chitinases