title: NMDA receptor upregulation: molecular studies in cultured mouse cortical neurons after chronic antagonist exposure.
abstract: We examined the possibility of changes in gene expression of the NMDA receptor subunits after chronic antagonist treatment. Exposure of neurons to the NMDA antagonist D(-)-2-amino-5-phosphonopentanoic acid (AP-5) produced an increase in the levels of the R2B mRNA subunit. Concomitant exposure of neurons to AP-5 and NMDA reversed the upregulation. Chronic AP-5 treatment increased the R1 polypeptide, whereas no change was observed in the levels of mRNA encoding the R1 subunit. A more pronounced increase was observed in the R2A/B polypeptides. These data demonstrate that chronic treatment with NMDA antagonists selectively upregulates the NMDA receptor mRNAs and polypeptides. Furthermore, antagonist treatment produced a differential regulation of the R1, R2A, and R2B subunits in cortical neurons.
authors.affiliation: Department of Pharmacology, University of Texas Health Science Center, San Antonio 78284-7764, USA.
authors.forename: P
authors.initials: P
authors.lastname: Follesa
authors.forename: M K
authors.initials: MK
authors.lastname: Ticku
mesh_terms: D015763:2-Amino-5-phosphonovalerate / Q000494:pharmacology*; D000818:Animals; D002478:Cells, Cultured / Q000502:physiology / Q000648:ultrastructure; D002540:Cerebral Cortex / Q000166:cytology; D015870:Gene Expression / Q000187:drug effects; D015151:Immunoblotting; D051379:Mice; D008810:Mice, Inbred C57BL; D009474:Neurons / Q000502:physiology / Q000648:ultrastructure; D010455:Peptides / Q000187:drug effects / Q000378:metabolism; D012333:RNA, Messenger / Q000187:drug effects / Q000378:metabolism; D016194:Receptors, N-Methyl-D-Aspartate / Q000037:antagonists & inhibitors / Q000502:physiology* / Q000648:ultrastructure; D013997:Time Factors; D015854:Up-Regulation / Q000187:drug effects / Q000235:genetics
chemical_list: D010455:Peptides; D012333:RNA, Messenger; D016194:Receptors, N-Methyl-D-Aspartate; D015763:2-Amino-5-phosphonovalerate