title: In vitro biological effects of clay minerals advised as substitutes for asbestos.
abstract: We studied one sample of commercial sepiolite and two samples of commercial vermiculite--clay minerals proposed as replacements for asbestos--and tested in vitro their abilities to activate complement, to lyse erythrocytes, and to elicit the production of reactive oxygen species (ROS) with human polymorphonuclear leukocytes (PMN) or bovine alveolar macrophages (AM); their behavior was compared with that of asbestos fibers obtained from the Union International Contra Cancer (UICC) as reference standards, as well as with kaolinite and illite, main members of the clay mineral family. Since in short-term in vitro tests the biological activity of mineral particles seems especially related to the active sites on their surface, we first measured the specific surface area of each mineral. Sepiolite was unreactive in two of the three tests we used (complement activation and ROS production) and able to lyse a minimal percentage of red blood cells. Vermiculite was shown to be incapable of activating complement, to have a moderate hemolytic activity and a high ability to elicite ROS production, although lower than that of chrysotile. Sepiolite, therefore, might be of more interest than vermiculite, given the low level of biological effects detected during the tests used to compare both clay minerals with asbestos fibres. The ROS production does not seem to require phagocytosis. A high ROS production was observed with kaolinite: this result casts doubt on the ability of pathogenic mineral dusts in vitro to induce a greater release of ROS than nonpathogenic mineral dusts.
authors.affiliation: Institute of Occupational Medicine, University of Ancona, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Governa
authors.forename: M
authors.initials: M
authors.lastname: Valentino
authors.forename: I
authors.initials: I
authors.lastname: Visonà
authors.forename: F
authors.initials: F
authors.lastname: Monaco
authors.forename: M
authors.initials: M
authors.lastname: Amati
authors.forename: G
authors.initials: G
authors.lastname: Scancarello
authors.forename: G
authors.initials: G
authors.lastname: Scansetti
mesh_terms: D000166:Acridines; D000538:Aluminum Silicates / Q000009:adverse effects*; D000818:Animals; D000863:Antacids / Q000009:adverse effects; D000930:Antidiarrheals / Q000009:adverse effects; D001194:Asbestos / Q000009:adverse effects*; D017638:Asbestos, Crocidolite / Q000009:adverse effects; D017632:Asbestos, Serpentine / Q000009:adverse effects; D002273:Carcinogens / Q000494:pharmacology; D002417:Cattle; D000077215:Clay; D003167:Complement Activation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D007616:Kaolin / Q000009:adverse effects; D016014:Linear Models; D008163:Luminescent Measurements; D008165:Luminol; D016676:Macrophages, Alveolar / Q000187:drug effects; D017633:Magnesium Silicates / Q000009:adverse effects*; D008903:Minerals / Q000009:adverse effects; D009504:Neutrophils / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000032:analysis; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D015054:Zymosan / Q000494:pharmacology
chemical_list: D000166:Acridines; D000538:Aluminum Silicates; D000863:Antacids; D000930:Antidiarrheals; D017632:Asbestos, Serpentine; D002273:Carcinogens; D017633:Magnesium Silicates; D008903:Minerals; D017382:Reactive Oxygen Species; D017638:Asbestos, Crocidolite; C003760:vermiculite; D001194:Asbestos; C033472:10,10'-dimethyl-9,9'-biacridinium; D007616:Kaolin; D008165:Luminol; D015054:Zymosan; C001671:magnesium trisilicate; D013755:Tetradecanoylphorbol Acetate; D000077215:Clay