title: Belactins A and B, new serine carboxypeptidase inhibitors produced by Actinomycete. I. Taxonomy, production, isolation and biological activities.
abstract: Belactins A and B, new inhibitors of serine carboxypeptidase were discovered in the fermentation broth of Saccharopolyspora sp. MK19-42F6. They were purified by ethyl acetate extraction, silica gel chromatography, Sephadex LH20 chromatography, Capcellpak C18 SG120 reversed phase HPLC and centrifugal partition chromatography (CPC) following their inhibitory activity against carboxypeptidase Y (CP-Y). The inhibition constants (Ki) of belactins A and B against CP-Y are 0.14 and 0.27 microM respectively. Belactins A and B have highly specific inhibitory activities for CP-Y among various peptidases, have no antimicrobial activities at 100 micrograms/ml and have low toxicities.
authors.affiliation: Institute of Microbial Chemistry, Tokyo, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Murakami
authors.forename: S
authors.initials: S
authors.lastname: Harada
authors.forename: F
authors.initials: F
authors.lastname: Kojima
authors.forename: N
authors.initials: N
authors.lastname: Kinoshita
authors.forename: Y
authors.initials: Y
authors.lastname: Takahashi
authors.forename: M
authors.initials: M
authors.lastname: Hamada
authors.forename: T
authors.initials: T
authors.lastname: Takeuchi
authors.forename: T
authors.initials: T
authors.lastname: Aoyagi
mesh_terms: D001549:Benzamides / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D002268:Carboxypeptidases / Q000037:antagonists & inhibitors*; D043402:Cathepsin A; D004305:Dose-Response Relationship, Drug; D005960:Glucosides / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D007783:Lactones / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D011480:Protease Inhibitors / Q000494:pharmacology*; D016930:Saccharopolyspora / Q000737:chemistry* / Q000145:classification
chemical_list: D001549:Benzamides; D005960:Glucosides; D007783:Lactones; D011480:Protease Inhibitors; C098701:belactin A; C098702:belactin B; D002268:Carboxypeptidases; D043402:Cathepsin A; C022633:serine carboxypeptidase