title: In vitro enhancement of antibody responses by T delta cells expressing IgD receptors.
abstract: Previous in vivo studies have shown that murine T cells induced to express receptors specific for IgD (IgD-R) have humoral immunoaugmenting properties with respect to both primary and secondary antibody responses to various antigens. Such murine IgD-R+ T cells (T delta cells) belong to the CD4+ T cell population in contrast with human T delta cells which also include CD8+ cells. The purpose of these studies was to develop an in vitro assay system to examine the mechanism by which T delta cells facilitate enhanced antibody responses. Our studies demonstrate that B cell responses to both soluble and particulate antigens can, indeed, be enhanced in vitro by coculture with T cells previously induced to express IgD-R. This in vitro effect requires cognate interaction between T and B cells and is dependent on the presence of adherent cells or IL-1. T cell priming with antigen, while not a prerequisite, was found to result in more effective T delta-B cell interactions compared with naive T delta cells. Finally, evidence was obtained in support of an adhesion-mediated T delta-B cell interaction since the immunoaugmenting properties of T cells expressing IgD-R are completely blocked by the addition of very low doses of monomeric IgD to the cultures.
authors.affiliation: Department of Microbiology and Immunology, CUNY Medical School, New York 10031, USA.
authors.forename: Y J
authors.initials: YJ
authors.lastname: Yang
authors.forename: R F
authors.initials: RF
authors.lastname: Coico
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D000921:Antibody-Producing Cells / Q000276:immunology* / Q000378:metabolism*; D000938:Antigen-Presenting Cells / Q000276:immunology; D002478:Cells, Cultured; D005260:Female; D007072:Immunoglobulin D / Q000737:chemistry / Q000494:pharmacology*; D051379:Mice; D011948:Receptors, Antigen, T-Cell / Q000096:biosynthesis; D011961:Receptors, Fc / Q000096:biosynthesis / Q000378:metabolism*; D015203:Reproducibility of Results; D013329:Structure-Activity Relationship; D013601:T-Lymphocytes / Q000145:classification / Q000276:immunology* / Q000378:metabolism
chemical_list: D000276:Adjuvants, Immunologic; D007072:Immunoglobulin D; D011948:Receptors, Antigen, T-Cell; D011961:Receptors, Fc; C050855:immunoglobulin D receptor