Effects of the CDC2 gene on adaptive mutation in the yeast Saccharomyces cerevisiae.
We have studied the influence of a temperature-sensitive cdc2-1 mutation in DNA polymerase delta on the selection-induced mutation occurring at the LYS-2 locus in the yeast Saccharomyces cerevisiae. It was found that in cells plated on synthetic complete medium lacking only lysine, the numbers of Lys+ revertant colonies accumulated in a time-dependent manner in the absence of any detectable increase in cell number. When cdc2-1 mutant cells, after selective plating, were incubated at the restrictive temperature of 37 degrees C for 5 h daily for 7 days, the frequency of an adaptive reversion of lys(-)-->Lys+ was significantly higher than the frequency in cells incubated only at the permissive temperature, or in wild-type cells incubated either at 23 degrees C or 37 degrees C. Therefore, when the proofreading activity of DNA polymerase delta is impaired under restrictive conditions, the frequency of adaptive mutations is markedly enhanced.
Baranowska
H
H
Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland.
Policińska
Z
Z
Jachymczyk
W J
WJ
D000222:Adaptation, Physiological / Q000235:genetics; D016203:CDC2 Protein Kinase / Q000235:genetics*; D002455:Cell Division; D004258:DNA Polymerase III; D004259:DNA-Directed DNA Polymerase / Q000235:genetics* / Q000378:metabolism; D016368:Frameshift Mutation; D008239:Lysine / Q000378:metabolism; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D012641:Selection, Genetic; D013696:Temperature; D013997:Time Factors
D016203:CDC2 Protein Kinase; D004258:DNA Polymerase III; D004259:DNA-Directed DNA Polymerase; D008239:Lysine