Use of two-hybrid system to identify Rab binding proteins.
Brondyk
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Promega Corporation, Madison, Wisconsin 53771, USA.
Macara
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D000818:Animals; D015153:Blotting, Western / Q000379:methods; D003001:Cloning, Molecular / Q000379:methods; D004268:DNA-Binding Proteins; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D004926:Escherichia coli; D005656:Fungal Proteins / Q000096:biosynthesis; D020558:GTP Phosphohydrolases / Q000096:biosynthesis* / Q000302:isolation & purification; D019204:GTP-Binding Proteins / Q000096:biosynthesis* / Q000302:isolation & purification; D015870:Gene Expression; D005822:Genetic Vectors; D005838:Genotype; D007202:Indicators and Reagents; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000302:isolation & purification; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D020798:Two-Hybrid System Techniques; D020692:rab3 GTP-Binding Proteins
D004268:DNA-Binding Proteins; D005656:Fungal Proteins; C052130:GAL4 protein, S cerevisiae; D007202:Indicators and Reagents; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D020558:GTP Phosphohydrolases; D019204:GTP-Binding Proteins; D020692:rab3 GTP-Binding Proteins