Pyridoxal phosphate induced association reactions of adrenodoxin and adrenodoxin-reductase.
Pyridoxal phosphate, cofactor of several enzymes, possesses linking properties to induce oligomerization of identical proteins such as adrenodoxin-reductase or adrenodoxin. The capability to get such self-assemblies is slightly lower than that for obtaining the heterologous complex between adrenodoxin-reductase and adrenodoxin which was found to be essential for electron transfer in the cytochrome P450 system. The influence of pyridoxal phosphate on the complex formation between adrenodoxin-reductase and adrenodoxin as well as the oligomerization reaction of the isolated proteins and possible consequences is discussed.
Behlke
J
J
Max-Delbrück-Centrum für Molekulare Medizin, Berlin-Buch, Germany.
Ristau
O
O
D000325:Adrenodoxin / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D000818:Animals; D005287:Ferredoxin-NADP Reductase / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D006860:Hydrogen Bonding; D007700:Kinetics; D008970:Molecular Weight; D011487:Protein Conformation; D011732:Pyridoxal Phosphate / Q000378:metabolism / Q000494:pharmacology*; D014461:Ultracentrifugation
D000325:Adrenodoxin; D011732:Pyridoxal Phosphate; D005287:Ferredoxin-NADP Reductase