Action of leukotriene B4 in human granulosa cells in vitro.
Leukotriene B4 directly enhanced progesterone release from superfused human granulosa cells. This secretory effect was observed in concentrations from 10(-12) to 10(-10) M. Lower and higher concentrations failed to affect progesterone release. When we analysed the high performance liquid chromatography profile of supernatant from human granulosa cell cultures, we detected a leukotriene B4 peak. In conclusion, these data support the hypothesis that leukotriene B4 may participate in the intracellular mechanism of progesterone release in human granulosa cells.
Rabe
T
T
Department of Obstetrics and Gynecology, University of Heidelberg, Germany.
Przylipiak
A
A
Grunwald
K
K
Parta
S
S
Przylipiak
M
M
Runnebaum
B
B
D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D004305:Dose-Response Relationship, Drug; D005260:Female; D006107:Granulosa Cells / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D007975:Leukotriene B4 / Q000494:pharmacology*; D009994:Osmolar Concentration; D011374:Progesterone / Q000378:metabolism*; D013997:Time Factors
D007975:Leukotriene B4; D011374:Progesterone