Studies on photoinactivation by various phthalocyanines of a free or replicating non-enveloped virus.
The non-enveloped picornaviruses, which are particularly resistant to physicochemical inactivation, include the aetiological agents of poliomyelitis, hepatitis A and E and infectious common cold (rhinovirus). In this work we used human rhinovirus type 5 (RV-5) cultivated in VERO cells to study the photoinactivating effects of several phthalocyanines and naphthobenzoporphyrazines. Free RV-5 was photoinactivated by aluminium trisulphonated naphthobenzoporphyrazine at 5 x 10(-8) M concentration. This photosensitizer was also active on replicating virus when the infected VERO cells were treated with 5 x 10(-6) M concentration followed by a very short illumination period. On the other hand, the ZnPc(3-MeO-Py)4 phthalocyanine, which possesses four positive charges, does not photoinactivate free rhinovirus, but this molecule protects VERO cells against RV-5 infection when added to the cultures before virus inoculation, in the presence or absence of subsequent illumination, and may therefore be considered as an antiviral agent in itself.
Gaspard
S
S
Institut de Chimie des Substances Naturelles, CNRS, Gif-sur-Yvette, France.
Tempête
C
C
Werner
G H
GH
D000818:Animals; D002522:Chlorocebus aethiops; D006801:Humans; D007211:Indoles / Q000494:pharmacology*; D054833:Isoindoles; D015394:Molecular Structure; D017319:Photosensitizing Agents / Q000494:pharmacology*; D012229:Rhinovirus / Q000187:drug effects* / Q000502:physiology / Q000528:radiation effects*; D014709:Vero Cells; D014779:Virus Replication
D007211:Indoles; D054833:Isoindoles; D017319:Photosensitizing Agents; C013647:phthalocyanine