Antiviral activity of recombinant interferon-alpha on hepatitis A virus replication in human liver cells.
Human recombinant interferon-alpha (IFN-alpha) was assayed for its antiviral effect on hepatitis A virus (HAV) replication in the human hepatoma cell line PLC/PRF/5. IFN-alpha resulted in concentration-dependent reduction of HAV antigen expression and HAV replication. IFN-alpha had a prophylactic effect, but was still effective when it was added after the infection, even at the end of the first replication cycle. An important increase in 2',5'-oligoadenylate synthetase activity in the IFN-treated human liver cells was observed. The antiviral effect of IFN-alpha could be attributed to the induction of this enzyme. Moreover we have shown that IFN-alpha and glycyrrhizin were synergistic in their antiviral actions against HAV. IFN-alpha emerged, from the present study, as a promising candidate for chemotherapy of severe forms of hepatitis A.
Crance
J M
JM
Unité de Virologie, Centre de Recherches du Service de Santé des Armées, La Tronche, France.
Lévêque
F
F
Chousterman
S
S
Jouan
A
A
Trépo
C
C
Deloince
R
R
D015088:2',5'-Oligoadenylate Synthetase / Q000378:metabolism; D000998:Antiviral Agents / Q000494:pharmacology*; D006034:Glycyrrhetinic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D019695:Glycyrrhizic Acid; D018963:Hepatitis Antigens / Q000032:analysis; D006507:Hepatovirus / Q000187:drug effects* / Q000276:immunology / Q000502:physiology; D006801:Humans; D016898:Interferon-alpha / Q000494:pharmacology*; D011993:Recombinant Fusion Proteins / Q000494:pharmacology; D013997:Time Factors; D014407:Tumor Cells, Cultured; D014779:Virus Replication / Q000187:drug effects
D000998:Antiviral Agents; D018963:Hepatitis Antigens; D016898:Interferon-alpha; D011993:Recombinant Fusion Proteins; D019695:Glycyrrhizic Acid; D015088:2',5'-Oligoadenylate Synthetase; D006034:Glycyrrhetinic Acid