Tissue cultures of Taxus baccata as a source of 10-deacetylbaccatin III, a precursor for the hemisynthesis of taxol.
Callus cultures of Taxus baccata L. cv. stricta were induced from hypocotyl and leaf explants on Woody Plant medium (hormone-free or additionated with phytohormones). Under continuous dark condition, adventitious roots were regenerated from hypocotyl- and leaf-derived callus cultures. Antibodies raised in rabbits against 10-succinyl-10-deacetylbaccatin III were used for the detection and the semi-quantitative determination of 10-deacetylbaccatin III in Taxus cultures. The presence of 10-deacetylbaccatin III in callus extracts was confirmed by TLC, HPLC using a photodiode array detector and mass spectrometry (CI-MS). The highest equivalent content of the taxoid derivatives (7.83 mg/100 g dry wt.) was detected in an extract from leaf-derived callus.
Zhiri
A
A
Laboratory of Plant Morphology, Université Libre de Bruxelles, Belgium.
Jaziri
M
M
Guo
Y
Y
Vanhaelen-Fastré
R
R
Vanhaelen
M
M
Homès
J
J
Yoshimatsu
K
K
Shimomura
K
K
D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D046508:Culture Techniques; D004797:Enzyme-Linked Immunosorbent Assay; D013058:Mass Spectrometry; D017239:Paclitaxel / Q000096:biosynthesis*; D018517:Plant Roots / Q000737:chemistry / Q000378:metabolism; D010946:Plants, Medicinal / Q000737:chemistry / Q000378:metabolism*
D017239:Paclitaxel