title: Identification, cloning and analysis of the Aspergillus niger gene pacC, a wide domain regulatory gene responsive to ambient pH.
abstract: A wide domain regulatory gene implicated in modulating gene expression in response to ambient pH has been cloned and sequenced from the industrially useful filamentous fungus Aspergillus niger. This gene, pacC, is able to restore a pacC+ phenotype to A. nidulans pacCc11 and pacCc14 mutants with respect to extent of conidiation, conidial pigment intensity and acid phosphatase regulation. The pacC gene of A. niger comprises three exons, encodes a three-zinc-finger protein of 677 amino acids, and shows pH-dependent regulation of expression: mRNA levels are elevated under alkaline conditions and considerably reduced under acidic conditions. The occurrence of PacC consensus binding targets within the sequences upstream of pacC may indicate autoregulation.
authors.affiliation: Section Molecular Genetics of Industrial Microorganisms, Wageningen Agricultural University, The Netherlands.
authors.forename: A P
authors.initials: AP
authors.lastname: MacCabe
authors.forename: J P
authors.initials: JP
authors.lastname: Van den Hombergh
authors.forename: J
authors.initials: J
authors.lastname: Tilburn
authors.forename: H N
authors.initials: HN
authors.lastname: Arst
authors.forename: J
authors.initials: J
authors.lastname: Visser
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism; D000595:Amino Acid Sequence; D001234:Aspergillus niger / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics; D015966:Gene Expression Regulation, Fungal / Q000235:genetics; D005800:Genes, Fungal; D005809:Genes, Regulator; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D010641:Phenotype; D014176:Protein Biosynthesis / Q000235:genetics; D015183:Restriction Mapping; D016415:Sequence Alignment; D014157:Transcription Factors / Q000737:chemistry* / Q000235:genetics*; D016335:Zinc Fingers / Q000235:genetics
chemical_list: D005656:Fungal Proteins; C092394:PacC protein, Aspergillus; D014157:Transcription Factors; D000135:Acid Phosphatase