Growth hormone does not prevent corticosteroid-induced changes in rat diaphragm structure and function.
The present study tested the hypothesis that growth hormone (GH), an anabolic agent, could prevent the abnormalities of diaphragm structure and function associated with short-term administration of the corticosteroid triamcinolone (TR). During a 10-day period, male rats (n = 33) were assigned to control (CTL), TR (1 mg.kg-1.day-1 im), and TR-GH (2 mg.kg-1.day-1 im) groups. Diaphragm weight was significantly reduced in the TR and TR-GH animals compared with the CTL animals, but there was no difference in the diaphragm-to-body weight ratio. Fiber type (I, IIa, and IIx/b) proportions did not differ among the three groups. However, in TR rats there was a significant reduction in the contribution of type IIx/b fibers to total diaphragm cross-sectional area due to marked atrophy (approximately 42% decrease in mean fiber cross-sectional area). There was no significant reversal of TR-induced type IIx/b fiber atrophy by concomitant GH administration. TR and TR-GH groups both exhibited a left-ward shift of the force-frequency relationship and enhanced in vitro fatigue resistance, whereas maximal specific force was unaltered. We conclude that GH does not prevent corticosteroid-induced effects on the diaphragm under these conditions, possibly as a result of reduced nutritional intake associated with TR administration.
Petrof
B J
BJ
Royal Victoria Hospital, Meakins-Christie Laboratories, McGill University, Montreal, Quebec, Canada.
Gottfried
S B
SB
Eby
J
J
Lamanca
J
J
Levine
S
S
D000818:Animals; D001835:Body Weight; D003964:Diaphragm / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D005938:Glucocorticoids / Q000009:adverse effects*; D013006:Growth Hormone / Q000494:pharmacology*; D007537:Isometric Contraction / Q000187:drug effects*; D008297:Male; D009218:Myosins / Q000378:metabolism*; D051381:Rats; D011916:Rats, Inbred F344; D014221:Triamcinolone / Q000009:adverse effects*
D005938:Glucocorticoids; D014221:Triamcinolone; D013006:Growth Hormone; D009218:Myosins