title: Hepatocyte nuclear factor 3beta participates in the transcriptional regulation of the thyroperoxidase promoter.
abstract: The promoters of the thyroglobulin (Tg) and thyroperoxidase (TPO) genes both contain sequences that match the consensus recognized by the fork-head family of transcription factors. The fork-head recognition element in the TPO promoter is recognized by two proteins, one of which is the transcription factor HNF-3beta, a member of the hepatocyte nuclear factor 3 (HNF-3) family, and the other is a thyroid-specific binding activity previously described as thyroid transcription factor 2 (TTF-2). Differently, only TTF-2 is capable of recognizing the fork-head recognition element in the thyroglobulin promoter, HNF-3beta protein is present in cultured thyroid cells and in the adult thyroid gland, suggesting that it does indeed have a role in modulating thyroid-specific gene expression. The transcriptional activities of TPO promoter mutants impaired in either TTF-2 or HNF-3beta binding suggest that both factors participate in regulating transcription from the TPO promoter.
authors.affiliation: Stazione Zoologica A. Dohrn, Villa Communale, 80121 Napoli, Italy.
authors.forename: K
authors.initials: K
authors.lastname: Sato
authors.forename: R
authors.initials: R
authors.lastname: Di Lauro
mesh_terms: D000818:Animals; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D002478:Cells, Cultured; D004247:DNA / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics*; D005786:Gene Expression Regulation; D006367:HeLa Cells; D051542:Hepatocyte Nuclear Factor 3-beta; D006801:Humans; D007453:Iodide Peroxidase / Q000235:genetics*; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics*; D015345:Oligonucleotide Probes / Q000235:genetics; D011401:Promoter Regions, Genetic; D051381:Rats; D013954:Thyroglobulin / Q000235:genetics; D013961:Thyroid Gland / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D004268:DNA-Binding Proteins; C496502:FOXA2 protein, human; C496504:Foxa2 protein, rat; D009687:Nuclear Proteins; D015345:Oligonucleotide Probes; D014157:Transcription Factors; D051542:Hepatocyte Nuclear Factor 3-beta; D004247:DNA; D013954:Thyroglobulin; D007453:Iodide Peroxidase