[Current data on the counting of weak leukocyte concentrations in labile blood products].
The precise measurement of low numbers of leukocyte below 0.1 WBC/microliter in filtered red cell or platelet suspensions meet both aims: to check the compliance with previously determined requirements and to evaluate the performances of novel filtering material (5 log depletion or more), justified by more and more important clinical use. The reliability of results, obtained with the chosen method, is ensured by applying of validation protocol, including training of technologist, assessment of the analytical range and the detection limit, assessment of precision and accuracy. The flow cytometry (FC) and Nageotte Chamber (NC) method are the both techniques which are currently used in routine Quality Control (QC) and validated by multicenter studies. Recent developments are made for increasing the sensibility of these counting methods, thanks to higher concentration or volume of the sample to be analysed. Among the experimental techniques, requiring more advances before implementing in QC program, quantitative PCR must become essential as reference method for evaluating the efficiency of filtration, in the future.
Masse
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Centre Régional de Transfusion Sanguine, Besançon.
D001781:Blood Component Removal / Q000592:standards*; D001792:Blood Platelets; D004912:Erythrocytes; D005374:Filtration / Q000295:instrumentation; D005434:Flow Cytometry; D006801:Humans; D007958:Leukocyte Count / Q000295:instrumentation / Q000379:methods*; D011786:Quality Control; D015203:Reproducibility of Results