title: CD14 mediate endotoxin induction of nitric oxide synthase in cultured brain glial cells.
abstract: Here we show that lipopolysaccharide (LPS) induction of glial inducible nitric oxide synthase (iNOS) requires membrane (m) and soluble (s) forms of CD14. In glial cell cultures, an anti-rat CD14 monoclonal antibody detected CD14 protein in whole cells and cell lysates, and reduced LPS-dependent iNOS expression. Glial cells and normal brain tissue expressed CD14 mRNA, as revealed by isolation of a rat CD14 clone (rCD14) from an astrocyte cDNA library and RT-PCR analysis. Finally, serum the ED(50) of LPS required for glial iNOS expression, and antibodies against sCD14 blocked the potentiating effect of serum.
authors.affiliation: Division of Neurobiology, Department of Neuroscience and Neurology, Cornell University Medical College, New York 10021, USA.
authors.forename: E
authors.initials: E
authors.lastname: Galea
authors.forename: D J
authors.initials: DJ
authors.lastname: Reis
authors.forename: E S
authors.initials: ES
authors.lastname: Fox
authors.forename: H
authors.initials: H
authors.lastname: Xu
authors.forename: D L
authors.initials: DL
authors.lastname: Feinstein
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001253:Astrocytes / Q000201:enzymology*; D001483:Base Sequence; D002478:Cells, Cultured; D004790:Enzyme Induction; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D018950:Lipopolysaccharide Receptors / Q000235:genetics / Q000502:physiology*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008969:Molecular Sequence Data; D019001:Nitric Oxide Synthase / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats
chemical_list: D007106:Immune Sera; D018950:Lipopolysaccharide Receptors; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D019001:Nitric Oxide Synthase