Leucocyte interleukin-1-like activity in the common seal (Phoca vitulina) and grey seal (Halichoerus grypus).
Interleukin-1 (IL-1) is an important cytokine with predominantly proinflammatory activities, which have been characterized in many mammals. This study showed the production of IL-1-like bioactivity by cultured seal leucocytes. Increasing concentrations of lipopolysaccharide (LPS) (0-1 micrograms/ml) stimulated an increase in measurable IL-1-like activity in cell culture supernates. This activity increased for the first 24 h after LPS stimulation and the substance responsible had an apparent molecular weight of 17 kDa on gel filtration, similar to that described for other species. Specificity of the bioassay used was confirmed by blocking the bioactivity with an IL-1 receptor antagonist (IL-1 ra).
King
D P
DP
Division of Clinical Sciences, Old Medical School, University of Leeds, UK.
Hay
A W
AW
Robinson
I
I
Evans
S W
SW
D000818:Animals; D001681:Biological Assay; D002478:Cells, Cultured; D005591:Chemical Fractionation; D002850:Chromatography, Gel; D007375:Interleukin-1 / Q000097:blood* / Q000737:chemistry; D007962:Leukocytes / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008970:Molecular Weight; D009994:Osmolar Concentration; D046048:Seals, Earless / Q000097:blood*; D012680:Sensitivity and Specificity; D013997:Time Factors
D007375:Interleukin-1; D008070:Lipopolysaccharides