Effect of hypergastrinemia and blockade of gastrin-receptors on pancreatic growth in the mouse.
OBJECTIVE
The present study evaluates whether endogenous gastrin regulates pancreatic growth in the mouse.


METHODS
Male NMRI mice weighing 12-15 g were assigned to six groups (10 mice/group) which were treated with different combinations of 0.9% NaCl, omeprazole, a CCK-A antagonist, a CCK-B antagonist, loxiglumide, and L365, 260 for 10 days each according to different protocols.


RESULTS
Omeprazole caused a marked, 10-fold increase in serum gastrin which was not affected by the gastrin antagonist, but markedly reduced by the CCK-A antagonist. The marked increase in endogenous gastrin caused by omeprazole did not promote pancreatic growth in any way. Similarly, the gastrin antagonist did not inhibit pancreatic growth. In contrast, the CCK-A antagonist significantly decreased pancreatic weight and protein content.


CONCLUSIONS
The present results strongly suggest that endogenous gastrin--in contrast to CCK--does not regulate pancreatic growth in the mouse. The inhibitory effect of loxiglumide on omeprazole-induced increase in serum gastrin might be explained by recent findings which showed that CCK-A antagonists can stimulate gastric acid secretion probably due to a reduction of the inhibitory effect of basal CCK on the D-cell and its somatostatin release. Probably such a slight stimulation of gastric acid secretion caused by the CCK-A antagonist might reduce the gastrin increase caused by omeprazole's abolishment of acid secretion.
Niederau
C
C
Department of Medicine, Heinrich-Heine-University of Düsseldorf, Germany.
Niederau
M
M
Klonowski
H
H
Lüthen
R
R
Ferrell
L D
LD
D000818:Animals; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005755:Gastrins / Q000097:blood / Q000502:physiology*; D006727:Hormone Antagonists / Q000494:pharmacology; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009853:Omeprazole / Q000494:pharmacology; D010179:Pancreas / Q000187:drug effects / Q000254:growth & development*; D011377:Proglumide / Q000031:analogs & derivatives / Q000494:pharmacology; D011949:Receptors, Cholecystokinin / Q000037:antagonists & inhibitors*
D004791:Enzyme Inhibitors; D005755:Gastrins; D006727:Hormone Antagonists; D011949:Receptors, Cholecystokinin; C053737:loxiglumide; D011377:Proglumide; D009853:Omeprazole