title: Secretion of IL-16 (lymphocyte chemoattractant factor) from serotonin-stimulated CD8+ T cells in vitro.
abstract: At sites of inflammation, mononuclear cells are in close contact with aggregated platelets. Although the physiologic role of this association is not clear, this proximity suggests that platelet-derived mediators may play a role in chemoattraction of T lymphocytes. In the current study we investigated serotonin receptor-bearing lymphocyte modulation of T cell migration. Serotonin-stimulated human blood mononuclear cells secrete lymphocyte chemoattractant activity with selective activity for CD4+ T cells. This chemoattractant activity was observed within 2 h of exposure to serotonin and was blocked by serotonin type 2 receptor antagonists. Molecular sieve chromatography of supernatant from serotonin-stimulated PBMCs revealed a single peak of T cell chemoattractant activity with an apparent molecular mass of 56 kDa and a pl of 9.1. Neutralizing experiments with specific mAbs indicated that the serotonin-induced chemotactic factor was the previously characterized lymphocyte chemoattractant factor (LCF), recently designated IL-16. Serotonin induced secretion of IL-16 from CD8+, not CD4+, T cells which did not require the de novo protein synthesis. These studies suggest that serotonin, via serotonin type 2 receptors, may promote the recruitment of CD4+ T lymphocytes into an inflammatory focus.
authors.affiliation: Pulmonary Center, Boston University School of Medicine, MA 02118, USA.
authors.forename: S
authors.initials: S
authors.lastname: Laberge
authors.forename: W W
authors.initials: WW
authors.lastname: Cruikshank
authors.forename: D J
authors.initials: DJ
authors.lastname: Beer
authors.forename: D M
authors.initials: DM
authors.lastname: Center
mesh_terms: D018414:CD8-Positive T-Lymphocytes / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism*; D002630:Chemotactic Factors / Q000737:chemistry / Q000378:metabolism*; D002634:Chemotaxis, Leukocyte / Q000276:immunology; D006801:Humans; D019410:Interleukin-16; D007700:Kinetics; D016131:Lymphocyte Subsets / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D008222:Lymphokines / Q000378:metabolism*; D011985:Receptors, Serotonin / Q000737:chemistry; D012701:Serotonin / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D002630:Chemotactic Factors; D019410:Interleukin-16; D008222:Lymphokines; D011985:Receptors, Serotonin; D012701:Serotonin