Propofol binding to human blood proteins.
The binding of the intravenous anesthetic propofol (CAS 2078-54-8) to human native plasma, hemoglobin and serum albumin was studied by means of equilibrium dialysis. Propofol binding to plasma over the large concentration range from 0.04 to 150 micrograms/ml was independent on the substrate concentration and amounted 97.4-98.6%. Serum albumin and hemoglobin also showed a marked binding for propofol. A 4% solution of albumin bound 88.7% and hemoglobin 86.2% of the anesthetic. In studies with constant protein concentration and variation of the propofol concentration a decrease of the percentage bound with increasing substrate concentration was seen for hemoglobin, indicating saturable binding sites. The opposite was found for the interaction between propofol and albumin: increase of the binding extent with increasing substrate concentration.
Altmayer
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Clinic of Anesthesiology and Intensive Care Medicine, University of the Saarland, Homburg, Germany.
Büch
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Büch
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HP
D001798:Blood Proteins / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D003956:Dialysis; D006454:Hemoglobins / Q000378:metabolism; D006801:Humans; D015742:Propofol / Q000097:blood*; D011485:Protein Binding; D012709:Serum Albumin / Q000378:metabolism
D001798:Blood Proteins; D006454:Hemoglobins; D012709:Serum Albumin; D015742:Propofol