In vivo induction of apoptosis and immune responses in mice by administration of lipopolysaccharide from Porphyromonas gingivalis.
In vivo administration of Porphyromonas gingivalis lipopolysaccharide (Pg-LPS) to mice induced apoptosis before a specific immune response. Apoptosis was associated with the expression of immunoglobulin and Ia on B cells and of CD5 and several markers on T cells. Apoptosis peaked in the spleen and lymph nodes on day 2, and the second peak occurred in the thymus on day 9. Tumor necrosis factor alpha (TNF-alpha) could mediate apoptosis, because the serum TNF-alpha levels were significantly higher than those of controls at 1 day before apoptosis and recombinant murine TNF-alpha induced apoptosis. The apoptosis induced by Pg-LPS was similar to that induced by Escherichia coli LPS in its basic manner, but it was unique in the response of thymus T cells. It was suggested that Pg-LPS could induce apoptosis for the elimination of early nonspecific activated lymphocytes.
Isogai
E
E
Department of Preventive Dentistry, Health Sciences University of Hokkaido, Japan.
Isogal
H
H
Kimura
K
K
Fujii
N
N
Takagi
S
S
Hirose
K
K
Hayashi
M
M
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D017209:Apoptosis / Q000187:drug effects*; D007376:Interleukin-2 / Q000096:biosynthesis; D008070:Lipopolysaccharides / Q000276:immunology* / Q000633:toxicity; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D016966:Porphyromonas gingivalis / Q000276:immunology* / Q000472:pathogenicity; D013601:T-Lymphocytes / Q000187:drug effects; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D000907:Antibodies, Bacterial; D007376:Interleukin-2; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha