Formation of fluorophore of yeast D-glyceraldehyde-3-phosphate dehydrogenase in the solution of potassium iodide.
A new fluorescence property of yeast D-glyceraldehyde-3-phosphate dehydrogenase was detected after it had been incubated in KI solution. The fluorophore formed in Baker's yeast GAPDH fluoresced at 383 nm by excitation at 336 nm. The formation of the fluorophore depended on NAD+. Modification of Cys-149 with iodoacetic acid influenced some properties of the fluorescence. It appears that the fluorophore may be situated at or near the active site.
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Laboratory of Molecular Neurobiology and Developmental Biology, National Laboratory of Macromolecules, Institute of Biophysics Academia Sinica, Chaoyang District, Beijing, P.R. China.
Liu
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D005453:Fluorescence; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000737:chemistry*; D011193:Potassium Iodide; D012441:Saccharomyces cerevisiae / Q000201:enzymology*
D011193:Potassium Iodide; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases