Multiplication of VHS virus in insect cells.
Viral haemorrhagic septicaemia virus (VHSV) belongs to the rhabdovirus family and is a major pathogen in farmed rainbow trout. An insect cell culture traditionally used for production of recombinant proteins was found to be susceptible to VHS virus. At pH 6.2, VHSV multiplication induced formation of large syncytia similar to those obtained by baculovirus-induced expression of recombinant VHSV glycoprotein. The VHSV G protein produced in insect cells was smaller than G protein derived from fish cells. VHS virus produced in insect cells was still pathogenic to rainbow trout after 2 cell culture passages.
Lorenzen
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Danish Veterinary Laboratory, Arhus N, Denmark.
Olesen
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NJ
D000818:Animals; D000956:Antigens, Viral / Q000096:biosynthesis / Q000302:isolation & purification; D015153:Blotting, Western; D002213:Capsid / Q000032:analysis / Q000096:biosynthesis; D002460:Cell Line; D007313:Insecta / Q000821:virology*; D017686:Oncorhynchus mykiss / Q000821:virology*; D012209:Rhabdoviridae / Q000472:pathogenicity / Q000502:physiology*; D014758:Viral Core Proteins / Q000032:analysis / Q000096:biosynthesis; D014759:Viral Envelope Proteins / Q000096:biosynthesis* / Q000302:isolation & purification; D014763:Viral Matrix Proteins / Q000032:analysis / Q000096:biosynthesis
D000956:Antigens, Viral; C096089:G protein, viral hemorrhagic septicemia virus; D014758:Viral Core Proteins; D014759:Viral Envelope Proteins; D014763:Viral Matrix Proteins