title: Random amplified polymorphic DNA analysis of clinically and environmentally isolated Cryptococcus neoformans in Nagasaki.
abstract: We examined clinical (pulmonary cryptococcosis and cryptococcal meningitis) and environmental (pigeon excreta) isolates of Cryptococcus neoformans var. neoformans (serotype A) in the southern Japanese prefecture of Nagasaki. The random amplified polymorphic DNA profiles obtained by using three primers revealed six patterns among 21 clinical isolates and three patterns among 8 environmental isolates. Pattern I was the most common (18 of 29 isolates) and was found among isolates obtained throughout the entire Nagasaki Prefecture. Patterns I, III, and IV were found among both clinical and environmental isolates. Patterns I and IV had a characteristic distribution, and in particular, pattern IV was isolated exclusively (five of six isolates) from isolates from Nagasaki City. Two environmental isolates from two locations associated strongly with two patients revealed identical random amplified polymorphic DNA patterns (patterns I and IV) for isolates from each patient. Our results suggest that clinical and environmental isolates belong to the same pool of C. neoformans isolates and that these isolates have certain geographic locations, although the number of isolated strains was limited.
authors.affiliation: Second Department of Internal Medicine, Nagasaki University School of Medicine, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Yamamoto
authors.forename: S
authors.initials: S
authors.lastname: Kohno
authors.forename: H
authors.initials: H
authors.lastname: Koga
authors.forename: H
authors.initials: H
authors.lastname: Kakeya
authors.forename: K
authors.initials: K
authors.lastname: Tomono
authors.forename: M
authors.initials: M
authors.lastname: Kaku
authors.forename: T
authors.initials: T
authors.lastname: Yamazaki
authors.forename: M
authors.initials: M
authors.lastname: Arisawa
authors.forename: K
authors.initials: K
authors.lastname: Hara
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001483:Base Sequence; D003453:Cryptococcosis / Q000453:epidemiology / Q000382:microbiology; D003455:Cryptococcus neoformans / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D017931:DNA Primers / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics*; D005260:Female; D006801:Humans; D007564:Japan / Q000453:epidemiology; D008172:Lung Diseases, Fungal / Q000453:epidemiology / Q000382:microbiology; D008297:Male; D016919:Meningitis, Cryptococcal / Q000453:epidemiology / Q000382:microbiology; D008875:Middle Aged; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D019105:Random Amplified Polymorphic DNA Technique
chemical_list: D017931:DNA Primers; D004271:DNA, Fungal