title: Canine distemper virus from diseased large felids: biological properties and phylogenetic relationships.
abstract: Specific pathogen free (SPF) domestic cats were inoculated with tissue homogenate obtained from a Chinese leopard (Panthera pardus japonensis) that had died in a North American zoo from a natural infection with canine distemper virus (CDV). The cats developed a transient cell-associated CDV viraemia along with pronounced lymphopenia but did not show any clinical symptoms. Plasma neutralizing-antibody titres against the homologous CDV (A92-27/4, isolated from the Chinese leopard) were consistently higher than against the CDV vaccine strain 'Bussell'. The Chinese leopard CDV isolate showed in vitro biological properties reminiscent of virulent, wild-type CDV strains. Sequence analysis of the H gene of two large felid CDV isolates from the USA (A92-27/4 and A92-6) revealed up to 10% amino acid changes including up to four additional potential N-linked glycosylation sites in the extra-cytoplasmic domain as compared to CDV vaccine strains. Phylogenetic analysis was performed using the entire coding region of the H gene and a 388 bp fragment of the P gene of several morbillivirus species. Evidence was obtained that recent CDV isolates from different species in the United States (including isolates from large felids), Europe and Africa are significantly distinct from CDV vaccine strains. All wild-type CDV isolates analysed clustered according to geographical distribution rather than to host species origin. By sequence analysis a CDV epizootic among large felids in a Californian safari park was linked to a virus which most likely originated from feral non-felid carnivores.
authors.affiliation: Department of Virology, Erasmus University, 3000 D R Rotterdam, The Netherlands.
authors.forename: T C
authors.initials: TC
authors.lastname: Harder
authors.forename: M
authors.initials: M
authors.lastname: Kenter
authors.forename: H
authors.initials: H
authors.lastname: Vos
authors.forename: K
authors.initials: K
authors.lastname: Siebelink
authors.forename: W
authors.initials: W
authors.lastname: Huisman
authors.forename: G
authors.initials: G
authors.lastname: van Amerongen
authors.forename: C
authors.initials: C
authors.lastname: Orvell
authors.forename: T
authors.initials: T
authors.lastname: Barrett
authors.forename: M J
authors.initials: MJ
authors.lastname: Appel
authors.forename: A D
authors.initials: AD
authors.lastname: Osterhaus
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002335:Carnivora / Q000821:virology*; D002415:Cats; D002522:Chlorocebus aethiops; D004279:DNA, Viral; D004216:Distemper / Q000821:virology*; D004217:Distemper Virus, Canine / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology; D004591:Electrophoresis, Polyacrylamide Gel; D005814:Genes, Viral; D008969:Molecular Sequence Data; D010802:Phylogeny; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D013047:Specific Pathogen-Free Organisms; D014709:Vero Cells; D014764:Viral Proteins / Q000032:analysis / Q000235:genetics*
chemical_list: D004279:DNA, Viral; D014764:Viral Proteins