title: Isolation of bone marrow plasma cells by negative selection with immunomagnetic beads.
abstract: In order to isolate bone marrow plasma cells from patients presenting with multiple myeloma or monoclonal gammopathy of undetermined significance, we developed a method for purifying these cells by negative selection using monoclonal antibodies and immunomagnetic beads. The results presented here were obtained from 75 procedures. Purity was extremely variable (2-100%) and was dependent on the percentage of plasma cells in the original bone marrow sample with a 10% cut-off, beyond which purity was over 96% in all cases. The mean yield was about 20%. The cells collected were viable and suitable for immunophenotyping, semi-quantitative studies of oncoproteins, and PCR.
authors.affiliation: Laboratoire d'Hématologie, CHU Rangueil, Toulouse, France.
authors.forename: G
authors.initials: G
authors.lastname: Fillola
authors.forename: C
authors.initials: C
authors.lastname: Muller
authors.forename: R
authors.initials: R
authors.lastname: Bousquet
authors.forename: A M
authors.initials: AM
authors.lastname: Fontanilles
authors.forename: P
authors.initials: P
authors.lastname: Laharrague
authors.forename: J X
authors.initials: JX
authors.lastname: Corberand
mesh_terms: D000911:Antibodies, Monoclonal; D001854:Bone Marrow Cells; D002469:Cell Separation / Q000379:methods*; D006801:Humans; D018189:Immunomagnetic Separation / Q000379:methods*; D008998:Monoclonal Gammopathy of Undetermined Significance / Q000473:pathology; D009101:Multiple Myeloma / Q000473:pathology; D010950:Plasma Cells / Q000166:cytology* / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal