PCR detection of cytomegalovirus DNA in serum as a diagnostic test for congenital cytomegalovirus infection.
PCR detected cytomegalovirus (CMV) DNA in the serum of 18 of 18 infants with symptomatic congenital CMV infection, 1 of 2 infants with asymptomatic congenital CMV infection, and 0 of 32 controls. Serum CMV PCR provided a rapid, sensitive, and specific method for diagnosis of congenital CMV infection in infants who were symptomatic at birth.
Nelson
C T
CT
Department of Pediatrics, Baylor College of Medicine, Houston, Texas, USA.
Istas
A S
AS
Wilkerson
M K
MK
Demmler
G J
GJ
D000914:Antibodies, Viral / Q000097:blood; D003587:Cytomegalovirus / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification*; D003586:Cytomegalovirus Infections / Q000151:congenital* / Q000175:diagnosis* / Q000821:virology; D004279:DNA, Viral / Q000097:blood* / Q000235:genetics*; D005260:Female; D006801:Humans; D007075:Immunoglobulin M / Q000097:blood; D007231:Infant, Newborn; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000706:statistics & numerical data; D012680:Sensitivity and Specificity; D014556:Urine / Q000821:virology
D000914:Antibodies, Viral; D004279:DNA, Viral; D007075:Immunoglobulin M