A possible correlation between interferon-stimulated gene expression and cytogenetic responses in chronic myelogenous leukemia patients treated with alpha-interferon.
We studied the expression of the interferon-stimulated genes (ISGs), ISG-54 and 2',5'-oligoadenylate synthetase (2'-5' OAS), using the reverse transcription-polymerase chain reaction in 22 patients with chronic myelogenous leukemia (CML), who were treated with alpha-interferon (alpha-IFN). At the time of diagnosis, 7 patients (31.8%) showed no detectable expression of either gene, whereas 8 expressed both. The remaining 7 expressed neither ISG-54 nor 2'-5' OAS. After alpha-IFN treatment, 5 of the 7 CML patients who had not previously express ISGs expressed either ISG-54 or 2'5' OAS or both and 6 of the 7 who had previously expressed either ISG-54 or 2'-5' OAS expressed both. Three of the 7 (42.9%) CML patients who expressed both genes before and after alpha-IFN administration showed cytogenetic responses, as did 6 of the 11 (54.5%) in whom ISG expression was induced. In contrast, cytogenetic responses occurred in none of the patients in whom ISG expression was not induced. These results suggest that induction of ISG-54 and 2'-5' OAS expression by alpha-IFN may be an indicator of cytogenetic responses and, therefore, of value for monitoring CML patients.
Kawakubo
K
K
First Department of Internal Medicine, Tokyo Medical College, Japan.
Ohyashiki
K
K
Ohyashiki
J H
JH
Shimamoto
T
T
Fujimura
T
T
Iwama
H
H
Toyama
K
K
D015088:2',5'-Oligoadenylate Synthetase / Q000235:genetics*; D000328:Adult; D051017:Apoptosis Regulatory Proteins; D001483:Base Sequence; D003582:Cytogenetics; D015971:Gene Expression Regulation, Enzymologic; D015973:Gene Expression Regulation, Leukemic; D006801:Humans; D016898:Interferon-alpha / Q000627:therapeutic use*; D007621:Karyotyping; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000201:enzymology / Q000235:genetics / Q000628:therapy*; D008969:Molecular Sequence Data; D010677:Philadelphia Chromosome; D016133:Polymerase Chain Reaction; D016601:RNA-Binding Proteins; D012074:Remission Induction; D014157:Transcription Factors / Q000235:genetics*
D051017:Apoptosis Regulatory Proteins; C484289:IFIT2 protein, human; D016898:Interferon-alpha; D016601:RNA-Binding Proteins; D014157:Transcription Factors; D015088:2',5'-Oligoadenylate Synthetase