Effect of vitamin A deficiency on the early response to experimental Pseudomonas keratitis.
OBJECTIVE
Vitamin A-deficient humans and animals are more susceptible to infections than are healthy humans and animals. This study compares the early corneal response (within 24 hours) to an experimental Pseudomonas aeruginosa infection between vitamin A deficient and control rats.


METHODS
Male WAG/Rij/MCW rats were fed either a vitamin A- deficient diet (A-) or the same diet with retinyl palmitate added back in a nonrestricted manner (N) or under pair-fed conditions (A+) to yield weight-matched rats. Some A-rats were repleted wih retinyl palmitate 16 days before being killed and then given free access to the retinyl palmitate-supplemented diet (R). Twenty-four hours before being killed, the corneas of anesthetized rats were scratched and P. aeruginosa organisms were applied to the corneal surface. The rats were killed using an overdose of sodium pentobarbital. Corneas were either processed for light and electron microscopic examination or extracted for proteinase and myeloperoxidase determination. Corneal myeloperoxidase concentrations relative to neutrophil myeloperoxidase concentrations were used to determine the number of neutrophils in the cornea. Zymography was used to study caseinases, gelatinases, and plasminogen activators. Reverse zymography was used to detect proteinase inhibitors. Similar results were noted at early, mid, and late weight plateau stages of vitamin A deficiency.


RESULTS
Ulceration occurred within 24 hours when low numbers of P. aeruginosa (10(4) cpu) were applied topically onto scratched A- corneas, whereas no ulceration was observed in the A+, R, and N corneas. When higher numbers of P. aeruginosa (10(7)-10(8)) were applied to the scratched corneas, all corneas became ulcerated within 24 hours. The extent of ulceration in the control corneas was greater than that in A- corneas by a factor of two. Only the A- corneas contained inflammatory cells with unusual striated deposits in phagolysosomes. The total number of neutrophils in the cornea and the concentrations of caseinases, plasminogen activators, and gelatinases in the infected corneal extracts were similar; however, the concentrations of cysteine proteinase inhibitors were elevated under A- conditions.


CONCLUSIONS
Vitamin A deficiency alters the response of the cornea to a P. aeruginosa infection during the first 24 hours. The alterations observed are probably due to multiple factors: an insufficient tear film for bacterial clearance and migration of neutrophils, epithelial keratinization, alterations in corneal wound healing, and changes in polymorphonuclear function.
Twining
S S
SS
Department of Biochemistry, Medical College of Wisconsin, Milwaukee, 53226, USA.
Zhou
X
X
Schulte
D P
DP
Wilson
P M
PM
Fish
B
B
Moulder
J
J
D000818:Animals; D016588:Anticarcinogenic Agents / Q000008:administration & dosage / Q000097:blood; D015153:Blotting, Western; D003315:Cornea / Q000201:enzymology / Q000473:pathology* / Q000648:ultrastructure; D003320:Corneal Ulcer / Q000201:enzymology / Q000382:microbiology / Q000473:pathology*; D004195:Disease Models, Animal; D004198:Disease Susceptibility; D004224:Diterpenes; D004591:Electrophoresis, Polyacrylamide Gel; D010450:Endopeptidases / Q000378:metabolism; D015818:Eye Infections, Bacterial / Q000201:enzymology / Q000473:pathology*; D005260:Female; D008099:Liver / Q000378:metabolism; D008297:Male; D009504:Neutrophils / Q000201:enzymology / Q000648:ultrastructure; D009195:Peroxidase / Q000378:metabolism; D011552:Pseudomonas Infections / Q000201:enzymology / Q000473:pathology*; D051381:Rats; D000084562:Retinyl Esters; D014801:Vitamin A / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000097:blood; D014802:Vitamin A Deficiency / Q000201:enzymology / Q000276:immunology* / Q000473:pathology
D016588:Anticarcinogenic Agents; D004224:Diterpenes; D000084562:Retinyl Esters; D014801:Vitamin A; C014794:retinol palmitate; D009195:Peroxidase; D010450:Endopeptidases