[Pituitary glycoprotein hormone receptors].
Monoclonal antibodies have been raised against the porcine LH receptor and have allowed to clone the corresponding messenger RNA from testicular cells. The stricture of the LH receptor has been determined. It shows similarities but also differences with other G protein coupled receptors. Specially a large extracellular domain is specific of that new family of receptors. Variant forms of the LH receptor generated by alternative splicing and lacking transmembrane domain have been isolated. Immunochemical and immunocytochemical studies have been performed. Three different forms of the LH receptor are physiologically expressed: a mature 85kDa transmembrane species, a 68 kDa high mannose containing species corresponding to a precursor which accumulates inside the cells, and truncated 45-48kDa molecular weight species corresponding to the variant messenger RNAs identified during the cloning of the receptor. A novel zonation of the ovary has been described by immunocytochemical studies. Cross hybridization with the LH receptor clone allowed to isolate the related TSH receptor from human thyroid tissue. The human LH and FSH receptor genes have been localized to chromosome 2p21 and the TSH receptor gene to chromosome 14q31. The genes are very large (> 60 kbp) and have introns only within the 5' part encoding the extracellular domain of the receptor. Immunoelectron microscopic studies performed in Leydig cells and in stably transfected L cells have allowed to study intracellular traffic of the LH receptor. The same approach was used to study the transendothelial transfer of hCG in testicular microvasculature.
Misrahi
M
M
Institut National de la Santé et de la Recherche Médicale, Hôpital de Bicêtre, Le Kremlin-Bicêtre.
Ghinea
N
N
Vu Hai
M T
MT
Loosfelt
H
H
Meduri
G
G
Atger
M
M
Gross
B
B
Jolivet
A
A
Milgrom
E
E
D000818:Animals; D003001:Cloning, Molecular; D004730:Endothelium, Vascular / Q000378:metabolism; D019204:GTP-Binding Proteins / Q000378:metabolism; D005796:Genes; D014644:Genetic Variation; D006801:Humans; D007150:Immunohistochemistry; D011962:Receptors, FSH / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D011974:Receptors, LH / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D011989:Receptors, Thyrotropin / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D013961:Thyroid Gland / Q000737:chemistry
D011962:Receptors, FSH; D011974:Receptors, LH; D011989:Receptors, Thyrotropin; D019204:GTP-Binding Proteins