Establishment of a bovine macrophage cell line from a fetal thymus.
A cell line, FBM-17, was established from a fetal bovine thymus and passaged for more than a year. Electron microscopic examination revealed that the cytoplasm contained many phagosomes and a significant amount of rough endoplasmic reticula. In addition, FBM-17 cells showed non-specific esterase, phagocytosis and surface expression of Fc receptors for IgG and classes I and II MHC antigens. These observations confirmed that this cell line had the morphologic and functional features as a macrophage. MHC expression markedly increased by treatment with recombinant bovine gamma-interferon, showing the cell line to be sensitive to bovine gamma-interferon and functional. These present results suggest that the FBM-17 is a functional bovine macrophage cell line. This line gamma-interferon, showing the cell line to be useful for clarification of roles of macrophages in immunoregulation in cattle.
Yoshihara
K
K
National Institute of Animal Health, Ibaraki, Japan.
Tanaka
S
S
Mori
Y
Y
Yokomizo
Y
Y
Onodera
T
T
Hirota
Y
Y
D000818:Animals; D002417:Cattle / Q000196:embryology*; D002460:Cell Line; D003593:Cytoplasm / Q000648:ultrastructure; D018870:Endoplasmic Reticulum, Rough / Q000648:ultrastructure; D007371:Interferon-gamma / Q000494:pharmacology; D008264:Macrophages / Q000737:chemistry / Q000166:cytology* / Q000648:ultrastructure; D008854:Microscopy, Electron / Q000662:veterinary; D011961:Receptors, Fc / Q000032:analysis; D011994:Recombinant Proteins / Q000494:pharmacology; D013950:Thymus Gland / Q000166:cytology* / Q000196:embryology*
D011961:Receptors, Fc; D011994:Recombinant Proteins; D007371:Interferon-gamma