[Detection of polyphosphatase activity in isolated Saccharomyces cerevisiae nuclei].
Intact nuclei have been isolated from cells of a diploid strain of Saccharomyces cerevisiae. The isolated nuclei were free from cytoplasmic, mitochondrial and vacuolar marker enzymes. The protein to DNA ratio (w/w) was 11. Pyrophosphatase, tripolyphosphatase and exopolyphosphatase activities have been found in S. cerevisiae nuclei for the first time and were equal to 400, 130 and 55 mU/mg of protein, respectively.
Lichko
L P
LP
Kulakovskaia
T V
TV
Dmitriev
V V
VV
Kulaev
I S
IS
D017766:Acid Anhydride Hydrolases / Q000378:metabolism*; D002467:Cell Nucleus / Q000201:enzymology* / Q000378:metabolism; D004271:DNA, Fungal / Q000378:metabolism; D005656:Fungal Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism
D004271:DNA, Fungal; D005656:Fungal Proteins; D017766:Acid Anhydride Hydrolases; C022563:endopolyphosphatase