Immunochemical characterization of the carbohydrate antigens of serotype k and Lancefield group G "Streptococcus milleri".
Carbohydrate antigens of the serotype k/Lancefield group G "Streptococcus milleri" were extracted by autoclaving whole cells of the type k reference strain Streptococcus anginosus K214-2K. The type k and group G antigen molecules are separated from each other and partially purified by a DEAE-Sephadex A25 column chromatography followed by a Sephadex G-100 gel filtration. In the double diffusion and the immunoelectrophoresis, the type k and group G antigen preparations obtained yielded single bands with their homologous antisera respectively. The type k antigen preparation contained principally glycerol, rhamnose, ribitol, galactose and glucose in a molar ratio of 0.54:1.20:0.43:0.33:1.00. The quantitative precipitin inhibition test indicated that beta(1-6)glucosyl-glucose (gentiobiose) sequence played a major role in immunodeterminant structure. Thus, the type k antigen of "S. milleri" appears to be a new carbohydrate type antigen that is chemically different from any Ottens type antigen. In contrast, the group G antigen preparation contained high proportion of rhamnose in addition to glucose, N-acetylglucosamine and N-acetylgalactosamine in a molar ratio of 6.45:1.00:0.24:1.15, and the rhamnose residue was involved in the immunodominant epitope, being in good agreement with the previously proposed chemical structure of the group antigen.
Kitada
K
K
Department of Preventive Dentistry, Kagoshima University Dental School, Japan.
Inoue
M
M
D000116:Acetylgalactosamine / Q000032:analysis / Q000276:immunology; D000117:Acetylglucosamine / Q000032:analysis / Q000276:immunology; D000942:Antigens, Bacterial / Q000737:chemistry* / Q000276:immunology*; D000954:Antigens, Surface / Q000737:chemistry / Q000276:immunology*; D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000737:chemistry / Q000276:immunology*; D002462:Cell Membrane / Q000737:chemistry / Q000276:immunology; D000939:Epitopes; D005690:Galactose / Q000032:analysis / Q000276:immunology; D005947:Glucose / Q000032:analysis / Q000276:immunology; D005990:Glycerol / Q000032:analysis / Q000276:immunology; D006241:Haptens / Q000276:immunology; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D008969:Molecular Sequence Data; D011233:Precipitin Tests; D012210:Rhamnose / Q000032:analysis / Q000276:immunology; D012255:Ribitol / Q000032:analysis / Q000276:immunology; D012703:Serotyping; D013291:Streptococcus / Q000145:classification* / Q000276:immunology*; D013682:Teichoic Acids / Q000276:immunology
D000942:Antigens, Bacterial; D000954:Antigens, Surface; D002241:Carbohydrates; D000939:Epitopes; D006241:Haptens; D013682:Teichoic Acids; D012255:Ribitol; D005947:Glucose; D000116:Acetylgalactosamine; D005990:Glycerol; D012210:Rhamnose; D000117:Acetylglucosamine; D005690:Galactose