Cloning and sequence analysis of the invertase gene INV 1 from the yeast Pichia anomala.
A genomic library from the yeast Pichia anomala has been constructed and employed to clone the gene encoding the sucrose-hydrolysing enzyme invertase by complementation of a sucrose non-fermenting mutant of Saccharomyces cerevisiae. The cloned gene, INV1, was sequenced and found to encode a polypeptide of 550 amino acids which contained a 22 amino-acid signal sequence and ten potential glycosylation sites. The amino-acid sequence shows significant identity with other yeast invertases and also with Kluyveromyces marxianus inulinase, a yeast beta-fructofuranosidase which has a different substrate specificity. The nucleotide sequences of the 5' and 3' non-coding regions were found to contain several consensus motifs probably involved in the initiation and termination of gene transcription.
Pérez
J A
JA
Departamento de Microbiología y Biología Celular, Facultad de Farmacia, Universidad de La Laguna, E-38071 La Laguna, Tenerife, Spain.
Rodríguez
J
J
Rodríguez
L
L
Ruiz
T
T
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D003062:Codon; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics*; D005800:Genes, Fungal; D006026:Glycoside Hydrolases / Q000737:chemistry / Q000235:genetics*; D006031:Glycosylation; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010843:Pichia / Q000201:enzymology / Q000235:genetics*; D021382:Protein Sorting Signals; D012091:Repetitive Sequences, Nucleic Acid; D017422:Sequence Analysis, DNA; D016385:TATA Box; D043324:beta-Fructofuranosidase
D003062:Codon; D005656:Fungal Proteins; D021382:Protein Sorting Signals; D006026:Glycoside Hydrolases; D043324:beta-Fructofuranosidase