title: Identification of human IgG autoantibodies specific for IL-10.
abstract: Since autoantibodies to IL-1alpha, interferon-alpha (IFN-alpha) and IL-6 have been described, this study concentrated on the search for autoantibodies to hIL-10 using an assay based on the precipitation of 125I-hIL-10 autoantibody complexes using Protein G-Sepharose. Among 1860 tested sera, only seven were found to specifically precipitate IL-10, thus indicating the rare occurrence of such autoantibodies. Four of those seven anti-IL-10 autoantibody sera were specific for hIL-10, two recognized both human and viral IL-10, while the last one recognized human, viral and murine IL-10, thus suggesting the existence of at least three different epitopic specificities. The purification of anti-IL-10 autoantibody from one serum demonstrated the existence of a single (IgG1, lambda) autoantibody that neutralized IL-10 biological activity. Thus, autoantibodies to IL-10 may represent natural antagonists to IL-10.
authors.affiliation: Schering Plough, Laboratory for Immunological Research, Dardilly, France.
authors.forename: C
authors.initials: C
authors.lastname: Menetrier-Caux
authors.forename: F
authors.initials: F
authors.lastname: Briere
authors.forename: P
authors.initials: P
authors.lastname: Jouvenne
authors.forename: E
authors.initials: E
authors.lastname: Peyron
authors.forename: F
authors.initials: F
authors.lastname: Peyron
authors.forename: J
authors.initials: J
authors.lastname: Banchereau
mesh_terms: D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions; D001323:Autoantibodies / Q000276:immunology*; D001327:Autoimmune Diseases / Q000276:immunology*; D018604:Epitope Mapping; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology*; D007132:Immunoglobulin Isotypes / Q000276:immunology; D016753:Interleukin-10 / Q000276:immunology*
chemical_list: D001323:Autoantibodies; D007074:Immunoglobulin G; D007132:Immunoglobulin Isotypes; D016753:Interleukin-10