title: [Genetic engineering of oxytocin: isolation of oxytocinoyllysine].
abstract: A hybrid protein, Il-Ox-K, was obtained from cells of E. coli TG1/pTOTEilox strain. The N-terminal sequence of this protein (63 amino acid residues) is a fragment of human interleukin-3, and the C-terminal sequence represents the full amino acid sequence of oxytocin flanked by a lysine residue. The modified oxytocinoyl-Lys containing S-sulfocysteine residues was isolated after tryptic digestion of S-sulfoderivative of the hybrid protein. The modified peptide was converted into the cyclic form containing the disulfide bonds [formula: see text]. Obtaining the oxytocinoyl-Lys proves the possibility of preparing short peptides using the microbiological synthesis.
authors.forename: T V
authors.initials: TV
authors.lastname: Ovchinnikova
authors.forename: T I
authors.initials: TI
authors.lastname: Murav'eva
authors.forename: L A
authors.initials: LA
authors.lastname: Chupova
authors.forename: A A
authors.initials: AA
authors.lastname: Tagaev
authors.forename: N Iu
authors.initials: NIu
authors.lastname: Martynova
authors.forename: A I
authors.initials: AI
authors.lastname: Miroshnikov
mesh_terms: D000595:Amino Acid Sequence; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D006801:Humans; D007377:Interleukin-3 / Q000235:genetics; D008969:Molecular Sequence Data; D010121:Oxytocin / Q000031:analogs & derivatives* / Q000235:genetics* / Q000302:isolation & purification; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D007377:Interleukin-3; D011993:Recombinant Fusion Proteins; C015332:oxytocin, Lys(8)-; D010121:Oxytocin