A novel Creb family gene telomeric of HLA-DRA in the HLA complex.
cDNA selection was used to identify genes encoded by a 440-kb yeast artificial chromosome (YAC) clone that spanned from HLA-DRA to CYP21 in the HLA complex. An initially selected short cDNA was used to isolate a 2639-nucleotide, apparently full-length cDNA from a human tonsil library. This cDNA contained one extended open reading frame that predicted a protein of 700 amino acids with a basic region and a leucine zipper that is highly similar to members of the Creb/ATF subfamily. High-stringency Southern blotting of total human genomic DNA using this cDNA as the probe showed only a single locus that mapped to the selecting YAC clone. This gene, designated Creb-related protein (Creb-rp), is expressed ubiquitously and is evolutionarily conserved in mammals. It is located in the HLA Class III region 6-10 kb centromeric of the XB gene, which encodes a tenascin-like extracellular matrix protein. Homologous sequences are located in the Class II-Class III interval of the mouse H-2 complex. The amino acid sequence homology and general structural features of the predicted protein indicate that this gene encodes a general transcription factor belonging to the Creb/ATF subfamily of the bZip super-family.
Min
J
J
Howard Hughes Medical Institute, Washington University School of Medicine, St Louis, Missouri 63110, USA.
Shukla
H
H
Kozono
H
H
Bronson
S K
SK
Weissman
S M
SM
Chaplin
D D
DD
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005075:Biological Evolution; D002461:Cell Line, Transformed; D018244:Chromosomes, Artificial, Yeast; D017362:Cyclic AMP Response Element-Binding Protein / Q000235:genetics*; D018076:DNA, Complementary; D006684:HLA-DR Antigens / Q000235:genetics*; D059805:HLA-DR alpha-Chains; D006801:Humans; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D016615:Telomere
D017362:Cyclic AMP Response Element-Binding Protein; D018076:DNA, Complementary; D006684:HLA-DR Antigens; D059805:HLA-DR alpha-Chains