Adjuvanticity of aluminium hydroxide and calcium phosphate in diphtheria-tetanus vaccines--I.
The potencies of two diphtheria-tetanus vaccines (DT) adsorbed to either aluminium hydroxide or calcium phosphate were compared in mice and guinea pigs. The vaccines were made from the same batches of purified toxoids and contained the same amounts of antigens. Immunizations were done once or twice with different doses of vaccine injected undiluted, diluted in saline or diluted in the corresponding adjuvant. The various potency assays showed that the adjuvanticity of calcium phosphate was lower than or equal to aluminium hydroxide. Despite the range of potency assays done, none of the methods reflected the efficacy of these vaccines in revaccination of humans. A simplified potency assay is suggested for release of final vaccine formulations to reduce the number of animals in quality control.
Aggerbeck
H
H
Statens Seruminstitut, Bacterial Vaccine Department, Copenhagen, Denmark.
Heron
I
I
D000276:Adjuvants, Immunologic / Q000378:metabolism / Q000494:pharmacology*; D000327:Adsorption; D000536:Aluminum Hydroxide / Q000378:metabolism / Q000494:pharmacology*; D000818:Animals; D002130:Calcium Phosphates / Q000378:metabolism / Q000494:pharmacology*; D004168:Diphtheria Toxoid / Q000378:metabolism / Q000494:pharmacology*; D022422:Diphtheria-Tetanus Vaccine; D005260:Female; D006168:Guinea Pigs; D007115:Immunization Schedule; D051379:Mice; D013745:Tetanus Toxoid / Q000378:metabolism / Q000494:pharmacology*; D017778:Vaccines, Combined / Q000494:pharmacology
D000276:Adjuvants, Immunologic; D002130:Calcium Phosphates; D004168:Diphtheria Toxoid; D022422:Diphtheria-Tetanus Vaccine; D013745:Tetanus Toxoid; D017778:Vaccines, Combined; C485828:alpha-tricalcium phosphate; C485830:tetracalcium phosphate; D000536:Aluminum Hydroxide; C485838:calcium phosphate, monobasic, anhydrous; C020243:calcium phosphate; C485829:calcium phosphate, dibasic, anhydrous