A high degree of sequence homology in the putative carbohydrate recognition domains of pokeweed mitogen and wheat germ agglutinin: poly-N-acetyllactosamine-binding lectins from different species.
The complete amino acid sequence of a poly-N-acetyllactosamine-binding pokeweed mitogen 4 (Pa-4) was determined using a protein sequencer. After digestion of Pa-4 with endoproteinase Lys-C, Asp-N, Arg-C or Glu-C, the resulting peptides were separated by reversed-phase high-performance liquid chromatography (HPLC) and then subjected to sequence analysis using a protein sequencer. The complete amino acid sequence of Pa-4 was found to exhibit a high degree of homology with that of wheat germ agglutinin (WGA) regarding their overall sequences and the spatial arrangement of cysteine-glycine. Furthermore, the amino acid residues of WGA directly involved in carbohydrate-binding sites were found in the homologous region in Pa-4. This is the first report to show that lectins from different plant families (Phytolaccaceae for Pa-4 and Gramineae for WGA) possess homologous primary sequences.
Konami
Y
Y
Department of Cancer Biology and Molecular Immunology, Faculty of Pharmaceutical Sciences, University of Tokyo, Japan.
Uno
T
T
Fujii
M
M
Yamamoto
K
K
Osawa
T
T
Irimura
T
T
D000595:Amino Acid Sequence; D001665:Binding Sites / Q000235:genetics; D050260:Carbohydrate Metabolism; D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011043:Pokeweed Mitogens / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D014909:Wheat Germ Agglutinins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
D002241:Carbohydrates; D011043:Pokeweed Mitogens; D011134:Polysaccharides; D014909:Wheat Germ Agglutinins; C037199:poly-N-acetyllactosamine