Pattern formation in Drosophila head development: the role of the orthodenticle homeobox gene.
Significant progress has been made towards understanding how pattern formation occurs in the imaginal discs that give rise to the limbs of Drosophila melanogaster. Here, we examine the process of regional specification that occurs in the eye-antennal discs, which form the head of the adult fruitfly. We demonstrate genetically that there is a graded requirement for the activity of the orthodenticle homeobox gene in forming specific structures of the developing head. Consistent with this result, we show that OTD protein is expressed in a graded fashion across the disc primordia of these structures and that different threshold levels of OTD are required for the formation of specific subdomains of the head. Finally, we provide evidence suggesting that otd acts through the segment polarity gene engrailed to specify medial head development.
Royet
J
J
University of Pennsylvania School of Medicine, Department of Neuroscience, Philadelphia 19104, USA.
Finkelstein
R
R
D000818:Animals; D004330:Drosophila / Q000196:embryology* / Q000235:genetics*; D029721:Drosophila Proteins; D004475:Ectoderm / Q000502:physiology*; D015870:Gene Expression; D018507:Gene Expression Regulation, Developmental; D005801:Genes, Homeobox; D006257:Head / Q000196:embryology*; D018869:Heat-Shock Response; D006651:Histocytochemistry; D018398:Homeodomain Proteins / Q000235:genetics*; D007150:Immunohistochemistry; D007301:Insect Hormones / Q000235:genetics; D009024:Morphogenesis / Q000235:genetics; D009154:Mutation; D010641:Phenotype; D014157:Transcription Factors / Q000235:genetics
D029721:Drosophila Proteins; C063459:En protein, Drosophila; D018398:Homeodomain Proteins; D007301:Insect Hormones; D014157:Transcription Factors; C093053:oc protein, Drosophila