Technical performance evaluation of BM/Hitachi 747-200 serum fructosamine assay.
The technical performance of serum fructosamine assay as a short-term index of diabetic control was evaluated by using the BM/Hitachi 747-200 Automatic Analyzer (Boehringer Mannheim Corp). Intra- and interassay precisions (CV) were <2%. Linearity was confirmed up to 1000 micromol/L. Lipemia, produced artificially or from hypertriglyceridemia in vivo, did not interfere with measurement of serum fructosamine concentration. However, hemoglobin (Hb) interfered with measurement substantially at concentrations >15.5 micromol/L, and bilirubin interfered substantially at concentrations >68.4 micromol/L. A normal reference range of up to 282 micromol/L (mean +/- 2SD = 242 +/- 40) was derived from results obtained from 228 nondiabetic subjects. In 222 diabetic subjects, serum fructosamine concentration correlated well with fasting glucose concentration (r = 0.74) and with Hb A1c concentration (r = 0.80). This automated fructosamine assay has the advantages of technical simplicity, low cost, and reduced analytical time compared with the Hb A1c method.
Lin
M J
MJ
Department of Chemistry, The Permanente Medical Group, Inc., Berkeley, CA 94710-1798, USA.
Hoke
C
C
Ettinger
B
B
Coyne
R V
RV
D001322:Autoanalysis / Q000295:instrumentation*; D001663:Bilirubin / Q000097:blood; D001786:Blood Glucose / Q000378:metabolism; D003920:Diabetes Mellitus / Q000097:blood*; D019270:Fructosamine; D006595:Hexosamines / Q000097:blood*; D006801:Humans; D008055:Lipids / Q000097:blood; D012016:Reference Values; D012680:Sensitivity and Specificity; D014280:Triglycerides / Q000097:blood
D001786:Blood Glucose; D006595:Hexosamines; D008055:Lipids; D014280:Triglycerides; D019270:Fructosamine; D001663:Bilirubin