Analysis of the renin gene in patients with aldosterone-producing adenomas by polymerase chain reaction-single stranded conformational polymorphisms and long polymerase chain reaction.
1. Angiotensin-responsive aldosterone-producing adenomas (AII-R-APA) have increased expression of renin mRNA compared with angiotensin-unresponsive aldosterone-producing adenomas (AII-U-APA) or normal adrenals. 2. Further, significant associations between the BglI, TaqI and HinfI RFLP and aldosterone responsiveness to the renin-angiotensin system of the two subgroups of patients have been reported. 3. Using the polymerase chain reaction based technique single stranded conformational polymorphism, we detected no alterations in exon 1 of the renin gene in peripheral blood leucocyte DNA from normal AII-U-APA and AII-R-APA subjects. 4. Using long-PCR, we amplified a fragment of the renin gene consisting of a region covering 500 bp upstream of exon 1, exon 1 and intron A. No gross changes in this area of the renin gene were found in the three groups of subjects studied. However this does not exclude small alterations in this area.
Ballantine
D M
DM
Hypertension Unit, Greenslopes Hospital, Brisbane, Queensland, Australia.
Klemm
S A
SA
Tunny
T J
TJ
Stowasser
M
M
Gordon
R D
RD
D000236:Adenoma / Q000150:complications / Q000235:genetics / Q000378:metabolism*; D000310:Adrenal Gland Neoplasms / Q000150:complications / Q000235:genetics / Q000378:metabolism*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000450:Aldosterone / Q000096:biosynthesis*; D001483:Base Sequence; D017931:DNA Primers / Q000737:chemistry; D004273:DNA, Neoplasm / Q000097:blood / Q000737:chemistry; D005091:Exons / Q000235:genetics; D005260:Female; D006801:Humans; D006929:Hyperaldosteronism / Q000209:etiology / Q000235:genetics* / Q000378:metabolism; D007438:Introns / Q000235:genetics; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D018807:Polymorphism, Single-Stranded Conformational; D012333:RNA, Messenger / Q000378:metabolism; D012083:Renin / Q000235:genetics*
D017931:DNA Primers; D004273:DNA, Neoplasm; D012333:RNA, Messenger; D000450:Aldosterone; D012083:Renin