title: On the possible origin of giant or slow-rising miniature end-plate potentials at the neuromuscular junction.
abstract: Giant or slow-rising miniature end-plate potentials (GMEPPs) caused by vesicular release of acetylcholine (ACh) occur at any time in about 50% of mouse diaphragm neuro muscular junctions, but generally at frequencies less than 0.03 s-1. Their frequency is, unlike that of miniature end-plate potentials (MEPPs), not affected by nerve terminal depolarization. Unlike MEPPs and stimulus-evoked end-plate potentials, GMEPPs have a prolonged time-to-peak and show an increase in time-to-peak with amplitude. By using these differences in amplitude and time course, GMEPPs can be separated from MEPPs. In contrast to MEPPs, GMEPPs are not blocked by botulinum neurotoxin type A. GMEPPs have a greater temperature sensitivity than MEPPs, disappearing at temperatures below 15 degrees C. Long-term paralysis by botulinum toxin and certain drugs which inhibit protein kinase C or affect actin filament polymerization (cytochalasins) enhance the frequency of GMEPPs. End-plate current recordings show that similar postsynaptic ACh receptors are activated by MEPPs and GMEPPs. It is suggested that GMEPPs are not caused by mechanisms involved in regulated neurotransmitter release but are generated by constitutive secretion.
authors.affiliation: Division of Biophysics, Department of Physical Sciences, University of Oulu, FIN-90570 Oulu, Finland.
authors.forename: L C
authors.initials: LC
authors.lastname: Sellin
authors.forename: J
authors.initials: J
authors.lastname: Molgó
authors.forename: K
authors.initials: K
authors.lastname: Törnquist
authors.forename: B
authors.initials: B
authors.lastname: Hansson
authors.forename: S
authors.initials: S
authors.lastname: Thesleff
mesh_terms: D000165:Acridine Orange / Q000494:pharmacology; D000818:Animals; D001905:Botulinum Toxins / Q000494:pharmacology; D003964:Diaphragm; D004640:Emetine / Q000494:pharmacology; D005071:Evoked Potentials / Q000502:physiology; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D051379:Mice; D009045:Motor Endplate / Q000187:drug effects / Q000502:physiology*; D009469:Neuromuscular Junction / Q000502:physiology*; D011493:Protein Kinase C / Q000187:drug effects / Q000378:metabolism; D011950:Receptors, Cholinergic / Q000502:physiology
chemical_list: D011950:Receptors, Cholinergic; D011493:Protein Kinase C; D001905:Botulinum Toxins; D000165:Acridine Orange; D004640:Emetine