Superoxide anion reduces the ability of myeloperoxidase to damage lipids.
The aim of this study was to determine whether lipids are damaged by myeloperoxidase (MP) not only with hydrogen peroxide (H2O2), but also with a H2O2-generating system. Using the hypoxanthine-xanthine oxidase-Cu,Zn-superoxide dismutase (HX-XO-SOD) system as an effective H2O2-generating system, we observed a reduction in the ability of MP to produce peroxylipids in the HX-XO-SOD-MP system, compared with the H2O2-MP system. We also noted that MP inhibited the production of monoepoxide by the H2O2-cytochrome c (Cyt c) system. These results suggest that MP plays a role as an absorber of active oxygen species and prevents phagocytes from self-destruction.
Iwase
H
H
Department of Forensic Medicine, Faculty of Medicine, University of Tokyo, Bunkyo-ku, Japan.
Takatori
T
T
Nagao
M
M
Iwadate
K
K
Takahashi
M
M
Nakajima
M
M
Takahashi
T
T
Shimizu
T
T
Takahashi
K
K
D003574:Cytochrome c Group / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D019271:Hypoxanthine; D007042:Hypoxanthines / Q000378:metabolism; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000378:metabolism*; D008054:Lipid Peroxides / Q000378:metabolism*; D009195:Peroxidase / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000378:metabolism; D013481:Superoxides / Q000494:pharmacology*; D014969:Xanthine Oxidase / Q000378:metabolism
D003574:Cytochrome c Group; D007042:Hypoxanthines; D008041:Linoleic Acids; D008054:Lipid Peroxides; D013481:Superoxides; D019271:Hypoxanthine; D019787:Linoleic Acid; D006861:Hydrogen Peroxide; D009195:Peroxidase; D013482:Superoxide Dismutase; D014969:Xanthine Oxidase