Immunoreactivities of PKC delta, eta, zeta in baker's yeast, lobster and wheat germ.
Varied immunoreactive bands of protein kinase C delta (PKC delta), PKC eta, and PKC zeta were detected in crude extracts of wheat germ, lobster tail meat, and three strains of baker's yeast by analysis of Western blots. Protease-deficient and Fleischmann's Active Dry yeasts exhibited immunoreactivity of PKC delta, whereas wheat germ and Fleischmann's RapidRise yeast displayed immunoreactivities of both PKC delta and PKC zeta. Lobster tail meat showed immunoreactivities of PKC eta and PKC zeta. These positive and negative immunoreactivities reflected evolutionary conservation and divergence, respectively, of these PKC isozymes in eukaryotes.
Kuo
W N
WN
Division of Science and Mathematics, Bethune-Cookman College, Daytona Beach, Florida 32114-3099, USA.
Ku
T W
TW
Jones
D L
DL
Jn-Baptiste
J B
JB
Bradshaw
C J
CJ
D000818:Animals; D015153:Blotting, Western; D007527:Isoenzymes / Q000032:analysis*; D008121:Nephropidae / Q000201:enzymology*; D011493:Protein Kinase C / Q000032:analysis; D051745:Protein Kinase C-delta; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D014908:Triticum / Q000201:enzymology*; D000099221:Protein Kinase C zeta
D007527:Isoenzymes; D011493:Protein Kinase C; D051745:Protein Kinase C-delta; D000099221:Protein Kinase C zeta; C081975:protein kinase C eta