Presence of interleukin-10 transcripts in human pituitary and hypothalamus.
1. Recent data have shown that interleukin-10 (IL-10) is expressed and acts in mouse pituitary tumor cells and freshly isolated mouse pituitaries. 2. In this study, we show that poly(A+) RNA derived from normal human pituitary and hypothalamus expresses IL-10 message. 3. The majority of transcripts was likely from the pituitary and hypothalamus, and not from lymphocytes in the pituitary and hypothalamic vasculature, since both IL-10 and interferon-gamma mRNA levels, compared to equivalent amounts of RNA from peripheral blood lymphocytes, were much lower. 4. These results indicate that IL-10 may function in human neuroendocrine process as it does in the murine system, thus serving as an important signal molecule for bidirectional communication between the neuroendocrine and the immune systems.
Rady
P L
PL
Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston 77555, USA.
Smith
E M
EM
Cadet
P
P
Opp
M R
MR
Tyring
S K
SK
Hughes
T K
TK
D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D017931:DNA Primers; D005260:Female; D015870:Gene Expression; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000096:biosynthesis; D006801:Humans; D007031:Hypothalamus / Q000276:immunology / Q000378:metabolism*; D007371:Interferon-gamma / Q000096:biosynthesis; D016753:Interleukin-10 / Q000096:biosynthesis*; D008214:Lymphocytes / Q000276:immunology / Q000378:metabolism; D008297:Male; D051379:Mice; D008875:Middle Aged; D009928:Organ Specificity; D010902:Pituitary Gland / Q000276:immunology / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis* / Q000378:metabolism; D012680:Sensitivity and Specificity; D014158:Transcription, Genetic
D017931:DNA Primers; D012333:RNA, Messenger; D016753:Interleukin-10; D007371:Interferon-gamma; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases