Increase of antigen production in BLV-infected cell lines via additional expression of tax.
The selection of animals infected with the bovine leukaemia virus (BLV) is performed by the immunological detection of antibodies against the virus, commonly using the antigen gp51. Furthermore, research is being carried out to develop protective vaccines against BLV that have gp51 as their main component. Taking both of these factors into account, it is clear that there will be an increasing requirement for the virus antigen gp51 for some time to come. The permanently BLV-infected foetal lamb kidney cell line FLK/BLV (and its sublines) has been proved to be the most useful culture for the mass production of the virus antigen. Stable cell lines producing higher quantities of BLV antigen have not been established, either by subcloning of the FLK/BLV or by infection of other permanent cells with BLV. Here, a report is made on efforts to increase the expression of gp51 in BLV-infected cells via the additional expression of homologous transactivating virus protein tax. Selectable tax expression vectors that integrate into the host cell genome were constructed using BL provirus DNA fragments. Highly productive FLK/BLV cells were transfected with these vectors. Following selection with G 418, gp51-producing cell lines were established and tested for their productivity for several months. Some tax-vector-containing cell lines have produced 1.5-2 times more gp51 than the highly productive parental control cell line FLK/BLV 44-1.
Wagner
H J
HJ
Free University Berlin, Department of Veterinary Medicine, Germany.
Blankenstein
P
P
Bondzio
A
A
Ebner
D
D
Risse
S
S
D000818:Animals; D000956:Antigens, Viral / Q000096:biosynthesis* / Q000235:genetics; D002460:Cell Line; D002522:Chlorocebus aethiops; D015925:Cryopreservation; D015967:Gene Expression Regulation, Viral; D016356:Gene Products, tax / Q000096:biosynthesis* / Q000235:genetics; D016355:Genes, pX; D001909:Leukemia Virus, Bovine / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D012756:Sheep; D014709:Vero Cells
D000956:Antigens, Viral; D016356:Gene Products, tax