title: Description of an automated method for the in vitro measurement of trypsinogen secretion from pancreatic segments.
abstract: The characterization of trypsinogen output from superfused pancreatic tissue by an automated spectrophotometric method is described. To test the method we investigated the time-course and the dose-response curve for acetylcholine-induced trypsinogen release from superfused pancreatic segments. We have demonstrated that this method allows the on-line detection and estimation of trypsinogen release with suitability, stability, and sensitivity.
authors.affiliation: Department of Physiology, University of Extremadura, Cáceres, Spain.
authors.forename: J I
authors.initials: JI
authors.lastname: San Román
authors.forename: J A
authors.initials: JA
authors.lastname: Tapia
authors.forename: J J
authors.initials: JJ
authors.lastname: Calvo
authors.forename: L J
authors.initials: LJ
authors.lastname: García
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D001322:Autoanalysis; D001331:Automation; D002138:Calibration; D004765:Enteropeptidase / Q000494:pharmacology; D006168:Guinea Pigs; D066298:In Vitro Techniques; D010179:Pancreas / Q000201:enzymology*; D012680:Sensitivity and Specificity; D013053:Spectrophotometry / Q000379:methods*; D014357:Trypsin / Q000378:metabolism / Q000494:pharmacology; D014362:Trypsinogen / Q000378:metabolism*
chemical_list: D014362:Trypsinogen; D014357:Trypsin; D004765:Enteropeptidase; D000109:Acetylcholine