title: A quantitative ultrastructural study of juxtaglomerular arterioles in IDDM patients with micro- and normoalbuminuria.
abstract: Hyalinization of juxtaglomerular arterioles is prominent in advanced diabetic nephropathy and may have important functional consequences. We studied the early stages of diabetic renal disease using kidney biopsy material from insulin-dependent diabetic patients, 8 with normal albumin excretion rate (< 15 micrograms/min) and 16 with microalbuminuria (15-200 micrograms/min). Ten living non-diabetic kidney donors served as a control group. Median duration of diabetes was 9.5 years (range 5-31) in patients with normoalbuminuria, and 12 years (7-22) in patients with microalbuminuria (p = 0.27). The tissue was sectioned systematically, 1-micron thick sections for light microscopy at 10-micron intervals, and thin sections for electron microscopy taken at 60-micron intervals. The arterioles were identified as afferent or efferent, and total profiles were photographed (magnification 7500x), providing a systematic independent sample for measurements using standard stereological methods. Patients with microalbuminuria had significantly increased arteriole parameters compared with the control group: for afferent and efferent arterioles the volume fraction of matrix/media, means and (co-efficient of variation, CV), was 0.47 (0.16) vs 0.33 (0.19) (p = 0.0009), and 0.62 (0.14) vs 0.45 (0.23) (p = 0.0004) and matrix-T, expressing amount of matrix per unit arteriolar surface, 2.38 (0.38) micron vs 1.44 (0.30) micron (p = 0.004), and 1.62 (0.28) micron vs. 1.03 (0.34) (p = 0.0009). Patients with normoalbuminuria showed no significant differences from the control group, and had lower values than microalbuminuric patients for all parameters except the afferent matrix-T. In the normoalbuminuric group a correlation was found between parameters for afferent arterioles and those for glomerular structure. In conclusion there is arteriolar accumulation of extracellular material in the early phase of diabetic nephropathy, concomitant with early glomerulopathy.
authors.affiliation: Medical Department M (Diabetes and Endocrinology, Arhus Kommunehospital, Denmark.
authors.forename: R
authors.initials: R
authors.lastname: Osterby
authors.forename: H J
authors.initials: HJ
authors.lastname: Bangstad
authors.forename: G
authors.initials: G
authors.lastname: Nyberg
authors.forename: J D
authors.initials: JD
authors.lastname: Walker
authors.forename: G
authors.initials: G
authors.lastname: Viberti
mesh_terms: D000293:Adolescent; D000328:Adult; D000419:Albuminuria / Q000473:pathology*; D001160:Arterioles / Q000648:ultrastructure; D016022:Case-Control Studies; D003922:Diabetes Mellitus, Type 1 / Q000473:pathology*; D003928:Diabetic Nephropathies / Q000473:pathology*; D005260:Female; D006801:Humans; D007606:Juxtaglomerular Apparatus / Q000098:blood supply* / Q000648:ultrastructure; D008297:Male