title: On the mechanism by which 6-ketocholestanol protects mitochondria against uncoupling-induced Ca2+ efflux.
abstract: This work shows that 6-ketocholestanol (kCh) inhibits the effect of carbonyl cyanide-m-chlorophenyl hydrazone (CCP) on mitochondrial Ca2+ efflux. Such an effect proved to be caused by diminution of membrane fluidity, therefore, it is affected by the incubation temperature. Furthermore, kCh reversed CCP-induced Ca2+ efflux depending on the accumulation of phosphate. It is also shown that kCh enhances the effect of carboxyatractyloside on membrane permeability transition.
authors.affiliation: Departamento de Bioquimica, Instituto Nacional de Cardiología, Mexico.
authors.forename: E
authors.initials: E
authors.lastname: Chávez
authors.forename: R
authors.initials: R
authors.lastname: Moreno-Sánchez
authors.forename: C
authors.initials: C
authors.lastname: Zazueta
authors.forename: A
authors.initials: A
authors.lastname: Cuéllar
authors.forename: J
authors.initials: J
authors.lastname: Ramirez
authors.forename: H
authors.initials: H
authors.lastname: Reyes-Vivas
authors.forename: C
authors.initials: C
authors.lastname: Bravo
authors.forename: S
authors.initials: S
authors.lastname: Rodríguez-Enríquez
mesh_terms: D000818:Animals; D001278:Atractyloside / Q000031:analogs & derivatives / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000037:antagonists & inhibitors / Q000494:pharmacology; D007425:Intracellular Membranes / Q000187:drug effects; D007653:Ketocholesterols / Q000494:pharmacology*; D007668:Kidney / Q000378:metabolism; D008560:Membrane Fluidity / Q000187:drug effects; D008928:Mitochondria / Q000378:metabolism*; D010539:Permeability / Q000187:drug effects; D051381:Rats; D014812:Vitamin K / Q000494:pharmacology
chemical_list: D007653:Ketocholesterols; D014812:Vitamin K; D001278:Atractyloside; C063833:6-ketocholestanol; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone; C003853:carboxyatractyloside; D002118:Calcium