title: In vitro replication of a reptilian adenovirus.
abstract: The morphological alterations of IgH-2 cells infected with a reptilian adenovirus were investigated during 1 cycle of virus replication. The virus particles entering the cell 1 h post infection (pi) were present in cytoplasmic receptosomes few hours later. About 24 h pi nucleocapsids entered the nucleus. Eosinophilic granulation of the nucleus and intra-nuclear inclusion bodies appeared between 30-60 h pi. Small round and ring-shaped (type I), circular, semicircular or rod-shaped (type II) and large, sharp, round (type III) inclusion bodies were formed. Typical crystalline aggregates of virions appeared at the periphery of the nucleus 48-60 h pi. The release of virions into the cytoplasm was followed by lysis of the infected cells. The destruction of cell monolayer was evident 96 h after infection.
authors.affiliation: Institute of Zoology, Fishery Biology and Fish Diseases, Veterinary Faculty of the University of Munich, Germany.
authors.forename: W
authors.initials: W
authors.lastname: Ahne
authors.forename: A
authors.initials: A
authors.lastname: Juhasz
mesh_terms: D000256:Adenoviridae / Q000302:isolation & purification / Q000502:physiology* / Q000648:ultrastructure; D000818:Animals; D002460:Cell Line; D002467:Cell Nucleus / Q000648:ultrastructure / Q000821:virology; D002479:Inclusion Bodies / Q000648:ultrastructure; D008854:Microscopy, Electron; D012911:Snakes / Q000821:virology*; D014779:Virus Replication