Electron spectroscopic imaging of encapsidated DNA in vaccinia virus.
We have used electron spectroscopic imaging to locate the phosphorus in vaccinia DNA in situ in unstained, ultrathin sections of virions. The phosphorus of the DNA backbone appeared to form a halo on the core periphery surrounding a phosphorus-impoverished central element. These results constrain models for how DNA could be packaged into mature vaccinia particles.
Harauz
G
G
Department of Molecular Biology and Genetics, University of Guelph, Canada.
Evans
D H
DH
Beniac
D R
DR
Arsenault
A L
AL
Rutherford
B
B
Ottensmeyer
F P
FP
D004279:DNA, Viral / Q000032:analysis / Q000737:chemistry / Q000648:ultrastructure*; D004577:Electron Probe Microanalysis; D008854:Microscopy, Electron; D010758:Phosphorus / Q000032:analysis; D014616:Vaccinia virus / Q000235:genetics* / Q000648:ultrastructure; D014771:Virion / Q000235:genetics / Q000648:ultrastructure
D004279:DNA, Viral; D010758:Phosphorus