Localization of apoptotic cells by direct immunogold detection of digoxigenin-labeled genomic DNA in semithin sections.
In this study, which correlates apoptosis with avian ovarian physiology, we modified an in situ DNA nick end-labeling method using immunogold reagents to detect apoptotic cells in semithin sections of quail ovaries embedded in glycol methacrylate resin. Special attention was paid to the prevention of background staining. The results are comparable with those of the ApopTag peroxidase kit in paraffin-embedded ovaries.
Van Nassauw
L
L
RUCA, Department of Morphology, Antwerp, Belgium.
Schrevens
A
A
Harrisson
F
F
Callebaut
M
M
D000818:Animals; D017209:Apoptosis; D003370:Coturnix; D004247:DNA / Q000032:analysis*; D004076:Digoxigenin / Q000737:chemistry; D005260:Female; D007150:Immunohistochemistry / Q000379:methods*; D010053:Ovary / Q000166:cytology*
D004247:DNA; D004076:Digoxigenin