title: Single-stranded DNA ligation by T4 RNA ligase for PCR cloning of 5'-noncoding fragments and coding sequence of a specific gene.
authors.affiliation: School of Forestry, Michigan Technological University, Houghton, MI 49931, USA.
authors.forename: X H
authors.initials: XH
authors.lastname: Zhang
authors.forename: V L
authors.initials: VL
authors.lastname: Chiang
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D011130:RNA Ligase (ATP)
chemical_list: D018076:DNA, Complementary; D011130:RNA Ligase (ATP)