title: Cysticercosis: identification and cloning of protective recombinant antigens.
abstract: We describe the cloning and the evaluation of the protective capacity of 5 recombinant antigens expressed during the cysticercus stage of both Taenia crassiceps and Taenia solium. A cDNA library was constructed in bacteriophage lambda ZAP using mRNA isolated from larvae of T. crassiceps of the ORF strain. The recombinant phage library was screened with polyclonal antibodies against 56- and 74-kDa protective antigen fractions. This screening identified 13 recombinant clones, 5 of which were also strongly recognized by pooled sera from pigs experimentally infected with T. solium. The native antigens are proteins of 56 (clones KETcl, 4, 7) and 74 and 78 kDa (clones KETc11, 12) of T. crassiceps cysticerci. Vaccination experiments using these 5 recombinant clones against murine cysticercosis point to the relevance of KETcl, 4, 7, and 12 in host protection, whereas immunization with the clone KETc11 does not modify the parasite load in females and facilitates the parasitosis in males. We report here the DNA and the deduced amino acid sequence (100 amino acids) of the first protective antigen (KETc7) of potential interest for T. solium pig cysticercosis prevention.
authors.affiliation: Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México.
authors.forename: K
authors.initials: K
authors.lastname: Manoutcharian
authors.forename: G
authors.initials: G
authors.lastname: Rosas
authors.forename: M
authors.initials: M
authors.lastname: Hernandez
authors.forename: G
authors.initials: G
authors.lastname: Fragoso
authors.forename: A
authors.initials: A
authors.lastname: Aluja
authors.forename: N
authors.initials: N
authors.lastname: Villalobos
authors.forename: L F
authors.initials: LF
authors.lastname: Rodarte
authors.forename: E
authors.initials: E
authors.lastname: Sciutto
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000947:Antigens, Helminth / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D010582:Bacteriophage lambda; D001483:Base Sequence; D003001:Cloning, Molecular; D003551:Cysticercosis / Q000517:prevention & control*; D003552:Cysticercus / Q000276:immunology*; D018509:DNA, Helminth / Q000737:chemistry; D005260:Female; D007114:Immunization; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D013552:Swine
chemical_list: D000947:Antigens, Helminth; D018509:DNA, Helminth; D011994:Recombinant Proteins