title: Effects of infused sodium lactate on glucose and energy metabolism in healthy humans.
abstract: To assess the effects of lactate on glucose metabolism, sodium lactate (20 mumol.kg-1.min-1) was infused into healthy subjects in basal conditions and during application of a hyperinsulinaemic (6 pmol.kg-1.min-1) euglycaemic clamp. Glucose rate of appearance (GRa) and disappearance (GRd) were measured from plasma dilution of infused U- 13C glucose, and glucose oxidation (G(ox)) from breath 13CO2 and plasma 13C glucose. In basal conditions, lactate infusion did not alter G(ox) (8.8 +/- 0.9 vs 9.2 +/- 1.1 mumol.kg-1.min-1), while GRa slightly decreased from 15.2 +/- 0.8 basal to 13.9 +/- 0.9 mumol.kg-1.min-1 after lactate (p < 0.05). During a hyperinsulinaemic clamp, hepatic glucose production was completely suppressed with or without lactate. Lactate decreased G(ox) from 17.1 +/- 0.4 to 13.4 +/- 1.2 mumol.kg-1.min-1 (p < 0.05), whereas GRd was unchanged (39.7 +/- 3.6 vs 45.6 +/- 2.6 mumol.kg-1.min-1. It is concluded that infusion of lactate in basal conditions does not increase GRa or interfere with peripheral glucose oxidation, and that during hyperinsulinaemia lactate decreases glucose oxidation but does not alter hepatic or peripheral insulin sensitivity.
authors.affiliation: Institut de physiologie, Lausanne, Switzerland.
authors.forename: N
authors.initials: N
authors.lastname: Paquot
authors.forename: P
authors.initials: P
authors.lastname: Schneiter
authors.forename: M C
authors.initials: MC
authors.lastname: Cayeux
authors.forename: R
authors.initials: R
authors.lastname: Chiolero
authors.forename: E
authors.initials: E
authors.lastname: Temler
authors.forename: E
authors.initials: E
authors.lastname: Jequier
authors.forename: L
authors.initials: L
authors.lastname: Tappy
mesh_terms: D000704:Analysis of Variance; D001481:Basal Metabolism; D001639:Bicarbonates / Q000097:blood / Q000652:urine; D001786:Blood Glucose / Q000378:metabolism*; D004734:Energy Metabolism / Q000187:drug effects*; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006946:Hyperinsulinism / Q000097:blood; D007700:Kinetics; D007773:Lactates / Q000494:pharmacology*; D019344:Lactic Acid; D008297:Male; D010084:Oxidation-Reduction; D012016:Reference Values
chemical_list: D001639:Bicarbonates; D001786:Blood Glucose; D007773:Lactates; D019344:Lactic Acid