Description of an automated method for the in vitro measurement of trypsinogen secretion from pancreatic segments.
The characterization of trypsinogen output from superfused pancreatic tissue by an automated spectrophotometric method is described. To test the method we investigated the time-course and the dose-response curve for acetylcholine-induced trypsinogen release from superfused pancreatic segments. We have demonstrated that this method allows the on-line detection and estimation of trypsinogen release with suitability, stability, and sensitivity.
San Román
J I
JI
Department of Physiology, University of Extremadura, Cáceres, Spain.
Tapia
J A
JA
Calvo
J J
JJ
García
L J
LJ
D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D001322:Autoanalysis; D001331:Automation; D002138:Calibration; D004765:Enteropeptidase / Q000494:pharmacology; D006168:Guinea Pigs; D066298:In Vitro Techniques; D010179:Pancreas / Q000201:enzymology*; D012680:Sensitivity and Specificity; D013053:Spectrophotometry / Q000379:methods*; D014357:Trypsin / Q000378:metabolism / Q000494:pharmacology; D014362:Trypsinogen / Q000378:metabolism*
D014362:Trypsinogen; D014357:Trypsin; D004765:Enteropeptidase; D000109:Acetylcholine