Nonhistone chromosomal proteins (NHCP) in adenocarcinoma of human endometrium.
Concentration and electrophoretic pattern of nonhistone chromosomal proteins (NHCP) in cancer and normal endometrium were examined. In normal tissues the NHCP/DNA ratio ranged from 0.6 to 0.8 whereas in malignant tissues from 1.2 to 1.7. Mean nuclear concentration of NHCP was 14.16 pg/cell nucleus of normal endometrium and 33.35 pg/cell nucleus of cancer tissue. The two tissues differed in the electrophoretic pattern. Heavy fractions (> 60 kDa--36%) predominated in normal tissue whereas light fractions (< 30 kDa--48%) in malignant tissue samples. The percentage of intermediate fractions (30-60 kDa) was similar. Neoplastic endometrium shows, irrespective of the patient's age, a high NHCP content. It seems that NHCP, especially the fraction extracted with 0.35 M NaCl, because of its tissue and cell specificity are involved in neoplasm formation.
Miturski
R
R
Second Clinic of Gynaecological Surgery Medical Academy of Lublin, Poland.
D000230:Adenocarcinoma / Q000737:chemistry*; D000328:Adult; D016022:Case-Control Studies; D002455:Cell Division / Q000502:physiology; D002868:Chromosomal Proteins, Non-Histone / Q000032:analysis*; D004586:Electrophoresis; D016889:Endometrial Neoplasms / Q000737:chemistry*; D005260:Female; D006801:Humans; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000032:analysis*
D002868:Chromosomal Proteins, Non-Histone; D009363:Neoplasm Proteins