title: Binding of matrix attachment regions to nuclear lamin is mediated by the rod domain and depends on the lamin polymerization state.
abstract: The nuclear matrix maintains specific interactions with genomic DNA at sites known as matrix attachment regions (M/SARs). M/SARs bind in vitro to lamin polymers. We show that the polymerized alpha-helical rod domain of lamin Dm0 provides by itself the specific binding to the ftz M/SAR. In contrast, unpolymerized rod domain does not bind specifically to this M/SAR. Non-specific binding to DNA is also observed with Dm0 containing a point mutation that impairs its ability to polymerize or with the isolated tail domain. These data suggest that the specific binding of lamins to M/SARs requires the rod domain and depends on the lamin polymerization state.
authors.affiliation: Department of Genetics, Life Sciences Institute, Hebrew University of Jerusalem, Israel.
authors.forename: K
authors.initials: K
authors.lastname: Zhao
authors.forename: A
authors.initials: A
authors.lastname: Harel
authors.forename: N
authors.initials: N
authors.lastname: Stuurman
authors.forename: D
authors.initials: D
authors.lastname: Guedalia
authors.forename: Y
authors.initials: Y
authors.lastname: Gruenbaum
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003460:Crystallization; D004247:DNA / Q000378:metabolism*; D029721:Drosophila Proteins; D004331:Drosophila melanogaster; D051717:Fushi Tarazu Transcription Factors; D018398:Homeodomain Proteins / Q000235:genetics; D034882:Lamins; D008969:Molecular Sequence Data; D015530:Nuclear Matrix / Q000378:metabolism*; D009687:Nuclear Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D017354:Point Mutation; D011108:Polymers / Q000378:metabolism; D011485:Protein Binding; D011487:Protein Conformation
chemical_list: D029721:Drosophila Proteins; D051717:Fushi Tarazu Transcription Factors; D018398:Homeodomain Proteins; C440328:Lam protein, Drosophila; D034882:Lamins; D009687:Nuclear Proteins; D011108:Polymers; C497169:ftz protein, Drosophila; D004247:DNA