title: Further validation of a quadruplex STR DNA typing system: a collaborative effort to identify victims of a mass disaster.
abstract: The relatively new, PCR-based technique of Short Tandem Repeat (STR) profiling has been used in the identification of the victims of a mass disaster. The analysis relied upon a recently developed multiplex reaction and the use of automated fluorescence technology to simulataneously analyse four tetrameric STR loci. The performance of the 'quadruplex' test was assessed by use of a collaborative study incorporating a blind trial and was demonstrated to be accurate, reliable and robust. Furthermore, the system proved to be highly successful despite the fact that many of the samples from the mass disaster scene were extremely degraded. The high success rate coupled with the discrimination power of the system enabled many severely decomposed human remains to be positively identified.
authors.affiliation: Forensic Science Service, Wetherby Laboratory, West Yorkshire, UK.
authors.forename: T M
authors.initials: TM
authors.lastname: Clayton
authors.forename: J P
authors.initials: JP
authors.lastname: Whitaker
authors.forename: D L
authors.initials: DL
authors.lastname: Fisher
authors.forename: D A
authors.initials: DA
authors.lastname: Lee
authors.forename: M M
authors.initials: MM
authors.lastname: Holland
authors.forename: V W
authors.initials: VW
authors.lastname: Weedn
authors.forename: C N
authors.initials: CN
authors.lastname: Maguire
authors.forename: J A
authors.initials: JA
authors.lastname: DiZinno
authors.forename: C P
authors.initials: CP
authors.lastname: Kimpton
authors.forename: P
authors.initials: P
authors.lastname: Gill
mesh_terms: D004247:DNA / Q000235:genetics; D005260:Female; D005390:Fires; D018732:Forensic Anthropology / Q000379:methods*; D005819:Genetic Markers / Q000235:genetics*; D006801:Humans; D008297:Male; D009679:Nuclear Family; D016133:Polymerase Chain Reaction; D012091:Repetitive Sequences, Nucleic Acid; D013781:Texas
chemical_list: D005819:Genetic Markers; D004247:DNA