Serogroup conversion of Vibrio cholerae non-O1 to Vibrio cholerae O1: effect of growth state of cells, temperature, and salinity.
Recently, we reported the occurrence of seroconversion from Vibrio cholerae non-O1 to V. cholerae O1, but little is known about the environmental and physiological factors influencing seroconversion. We investigated effects of temperature (4, 25, and 35 degrees C) and salinity ( < 0.05 and 10%0.), as well as the stage of growth of cells, on serogroup conversion. Seroconversion of V. cholerae occurred under various environmental conditions. However, the rate of seroconversion in natural water ( < 0.5% salinity) and synthetic seawater microcosms (10%0 salinity), employing cells harvested from stationary phase culture, was approximately 2 logs higher than cells harvested from cultures in the logarithmic phase (i.e., 10(5) versus 10(3) per 10(10) cells. Thus, the physiological state of the cells, and to a lesser degree, temperature and salinity, is an important factor in the conversion of V. cholerae from non-O1 to O1 serogroup.
Montilla
R
R
Department of Microbiology, University of Maryland, College Park 20742, USA.
Chowdhury
M A
MA
Huq
A
A
Xu
B
B
Colwell
R R
RR
D000942:Antigens, Bacterial / Q000096:biosynthesis* / Q000235:genetics; D003470:Culture Media / Q000494:pharmacology; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D019081:O Antigens / Q000096:biosynthesis*; D012623:Seawater; D012703:Serotyping; D013696:Temperature; D014734:Vibrio cholerae / Q000145:classification / Q000235:genetics / Q000276:immunology* / Q000472:pathogenicity; D014867:Water; D014871:Water Microbiology
D000942:Antigens, Bacterial; D003470:Culture Media; D019081:O Antigens; D014867:Water