title: Studies on the differential morphological alterations in human and goat erythrocytes against ATP depletion and Ca(2+)-induced stresses.
abstract: Erythrocyte membranes obtained from goat exhibited Ca2+/calmodulin-insensitive (Ca(2+)-Mg2+)-ATPase activity and the activity levels were significantly lower as compared to those obtained from human erythrocyte membranes. Although Ca2+/ionophore A23187 -treatment enhanced the intracellular Ca2+ levels in both the freshly collected human and goat erythrocytes, negligible loss of ATP was noticed in goat erythrocytes under such conditions. Also, fluoride, a known metabolic inhibitor, caused a lesser ATP depletion in goat erythrocytes compared to that of human. Goat erythrocytes showed remarkable rigidity to shape change and unlike human erythrocytes, did not undergo echinocytic formation as a result of metabolic depletion and Ca2+/ionophore A23187-treatments. These studies suggest the participation of (Ca(2+)-Mg2+)-ATPase -independent mechanism(s) for Ca(2+)-extrusion.
authors.affiliation: Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, India.
authors.forename: A
authors.initials: A
authors.lastname: Zaidi
authors.forename: M T
authors.initials: MT
authors.lastname: Khan
authors.forename: M
authors.initials: M
authors.lastname: Mirza
authors.forename: I
authors.initials: I
authors.lastname: Ahmad
authors.forename: M
authors.initials: M
authors.lastname: Saleemuddin
mesh_terms: D000255:Adenosine Triphosphate / Q000097:blood*; D000818:Animals; D017301:Ca(2+) Mg(2+)-ATPase / Q000097:blood*; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000494:pharmacology*; D002147:Calmodulin / Q000494:pharmacology; D003598:Cytoskeletal Proteins / Q000097:blood; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000201:enzymology / Q000648:ultrastructure; D004912:Erythrocytes / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D005459:Fluorides / Q000494:pharmacology; D006041:Goats / Q000097:blood*; D006801:Humans; D012016:Reference Values; D013045:Species Specificity; D013312:Stress, Physiological / Q000097:blood*
chemical_list: D002147:Calmodulin; D003598:Cytoskeletal Proteins; D000001:Calcimycin; D000255:Adenosine Triphosphate; D017301:Ca(2+) Mg(2+)-ATPase; D005459:Fluorides; D002118:Calcium