Immunoreactive endothelin in the human kidney: comparison with natriuretic peptides.
The presence of immunoreactive endothelin (IR-ET) was studied by radioimmunoassay in human kidney with and without clinical renal failure, and the levels were compared with those of three natriuretic peptides: atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP), and C-type natriuretic peptide (CNP). Kidney tissues were obtained at autopsy from three subjects with renal disorders (diabetic nephropathy, renal tubular necrosis, and end-stage kidney disease). Normal renal tissue was obtained at surgery from two patients with renal cell carcinoma. IR-ET was detected in three diseased kidneys obtained at autopsy (0.101 +/- 0.043 pmol/g wet weight, mean +/- SD) but not in tissues of two normal kidneys obtained at surgery (< 0.015 pmol/g wet weight). Reverse-phase HPLC showed that most of the IR-ET in the kidney (> 90%) was eluted in the position of ET-1. IR-ANP (0.23 +/- 0.06 pmol/g wet weight), IR-BNP (1.15 +/- 0.94 pmol/g wet weight), and IR-CNP (0.44 +/- 0.16 pmol/g wet weight) were detected in all samples examined. Higher concentrations of IR-BNP were found in three diseased kidneys obtained at autopsy (1.70 +/- 0.83 pmol/g wet weight vs. mean in two normal kidney tissues, 0.32 pmol/g wet weight). Such increases were not observed in IR-ANP or IR-CNP. These findings indicate that IR-ET is present in the human diseased kidney even in end-stage disease, with high concentrations comparable to those of natriuretic peptides. This raises the possibility that the production of ET-1 and BNP is increased in kidneys of patients with renal disorders.
Takahashi
K
K
Department of Applied Physiology and Molecular Biology, Tohoku University School of Medicine, Sendai, Japan.
Sone
M
M
Totsune
K
K
Satoh
F
F
Murakami
O
O
Ohneda
M
M
Mouri
T
T
D000368:Aged; D009320:Atrial Natriuretic Factor / Q000032:analysis* / Q000276:immunology; D002851:Chromatography, High Pressure Liquid; D016232:Endothelins / Q000032:analysis* / Q000276:immunology; D005260:Female; D006801:Humans; D007668:Kidney / Q000737:chemistry*; D008297:Male; D008875:Middle Aged; D020097:Natriuretic Peptide, Brain; D020098:Natriuretic Peptide, C-Type; D009419:Nerve Tissue Proteins / Q000032:analysis* / Q000276:immunology; D011506:Proteins / Q000032:analysis* / Q000276:immunology; D011863:Radioimmunoassay
D016232:Endothelins; D009419:Nerve Tissue Proteins; D011506:Proteins; D020097:Natriuretic Peptide, Brain; D020098:Natriuretic Peptide, C-Type; D009320:Atrial Natriuretic Factor