In vivo gene delivery and stable transduction of nondividing cells by a lentiviral vector.
A retroviral vector system based on the human immunodeficiency virus (HIV) was developed that, in contrast to a murine leukemia virus-based counterpart, transduced heterologous sequences into HeLa cells and rat fibroblasts blocked in the cell cycle, as well as into human primary macrophages. Additionally, the HIV vector could mediate stable in vivo gene transfer into terminally differentiated neurons. The ability of HIV-based viral vectors to deliver genes in vivo into nondividing cells could increase the applicability of retroviral vectors in human gene therapy.
Naldini
L
L
Salk Institute, La Jolla, CA 92037, USA.
Blömer
U
U
Gallay
P
P
Ory
D
D
Mulligan
R
R
Gage
F H
FH
Verma
I M
IM
Trono
D
D
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000166:cytology / Q000821:virology; D002455:Cell Division; D002478:Cells, Cultured; D005260:Female; D018014:Gene Transfer Techniques; D015316:Genetic Therapy; D005822:Genetic Vectors; D006678:HIV / Q000235:genetics* / Q000502:physiology; D006367:HeLa Cells; D006801:Humans; D008264:Macrophages / Q000166:cytology / Q000821:virology; D008969:Molecular Sequence Data; D009474:Neurons / Q000166:cytology / Q000821:virology; D010957:Plasmids; D051381:Rats; D014162:Transfection; D016662:Virus Integration