Role of Rho in chemoattractant-activated leukocyte adhesion through integrins.
Heterotrimeric guanine nucleotide binding protein (G protein)-linked receptors of the chemoattractant subfamily can trigger adhesion through leukocyte integrins, and in this role they are thought to regulate immune cell-cell interactions and trafficking. In lymphoid cells transfected with formyl peptide or interleukin-8 receptors, agonist stimulation activated nucleotide exchange on the small guanosine triphosphate-binding protein RhoA in seconds. Inactivation of Rho by C3 transferase exoenzyme blocked agonist-induced lymphocyte alpha4beta1 adhesion to vascular cell adhesion molecule-1 and neutrophil beta2 integrin adhesion to fibrinogen. These findings suggest that Rho participates in signaling from chemoattractant receptors to trigger rapid adhesion in leukocytes.
Laudanna
C
C
Laboratory of Immunology and Vascular Biology, Department of Pathology, Stanford University, CA 94305, USA.
Campbell
J J
JJ
Butcher
E C
EC
D000595:Amino Acid Sequence; D000818:Animals; D015703:Antigens, CD / Q000235:genetics; D001402:B-Lymphocytes / Q000502:physiology*; D002448:Cell Adhesion; D002478:Cells, Cultured; D002630:Chemotactic Factors / Q000494:pharmacology*; D019204:GTP-Binding Proteins / Q000378:metabolism / Q000502:physiology*; D016244:Guanosine 5'-O-(3-Thiotriphosphate) / Q000378:metabolism; D006153:Guanosine Diphosphate / Q000378:metabolism; D039041:Integrin alpha4beta1; D016023:Integrins / Q000502:physiology*; D016209:Interleukin-8 / Q000494:pharmacology; D051379:Mice; D008969:Molecular Sequence Data; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D044042:Receptors, Formyl Peptide; D011971:Receptors, Immunologic / Q000235:genetics; D018123:Receptors, Interleukin / Q000235:genetics; D023062:Receptors, Interleukin-8A; D016201:Receptors, Lymphocyte Homing / Q000502:physiology*; D018000:Receptors, Peptide / Q000235:genetics; D015398:Signal Transduction; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014162:Transfection; D019010:Vascular Cell Adhesion Molecule-1 / Q000502:physiology*; D020742:rhoA GTP-Binding Protein
D015703:Antigens, CD; D002630:Chemotactic Factors; D039041:Integrin alpha4beta1; D016023:Integrins; D016209:Interleukin-8; D044042:Receptors, Formyl Peptide; D011971:Receptors, Immunologic; D018123:Receptors, Interleukin; D023062:Receptors, Interleukin-8A; D016201:Receptors, Lymphocyte Homing; D018000:Receptors, Peptide; D019010:Vascular Cell Adhesion Molecule-1; D006153:Guanosine Diphosphate; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D009240:N-Formylmethionine Leucyl-Phenylalanine; D019204:GTP-Binding Proteins; D020742:rhoA GTP-Binding Protein; D013755:Tetradecanoylphorbol Acetate