Single-step PCR for detection of Brucella spp. from blood and milk of infected animals.
A versatile method for the extraction of Brucella DNA and PCR are presented as reliable tools for the detection of Brucella spp. from body fluids of infected animals. Two oligonucleotides homologous to regions of the gene encoding for an outer membrane protein (omp-2) were designed to detect the pathogen from milk and/or blood of infected goats, bovines, and human patients. The sensitivity of our test and its ability to detect the pathogen in samples from the field reveal a promising advance in the diagnosis of brucellosis in animals and humans.
Leal-Klevezas
D S
DS
Centro de Investigación Biomédica del Noreste, Instituto Mexicano del Seguro Social, Monterrey, Nuevo León, Mexico.
Martínez-Vázquez
I O
IO
López-Merino
A
A
Martínez-Soriano
J P
JP
D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics; D001483:Base Sequence; D001769:Blood / Q000382:microbiology*; D002002:Brucella / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D002003:Brucella abortus / Q000235:genetics / Q000302:isolation & purification; D017347:Brucella melitensis / Q000235:genetics / Q000302:isolation & purification; D002006:Brucellosis / Q000175:diagnosis / Q000662:veterinary; D002417:Cattle; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000097:blood / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D005798:Genes, Bacterial; D006041:Goats; D006801:Humans; D008892:Milk / Q000382:microbiology*; D008895:Milk, Human / Q000382:microbiology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000706:statistics & numerical data; D012680:Sensitivity and Specificity
D001425:Bacterial Outer Membrane Proteins; D017931:DNA Primers; D004269:DNA, Bacterial