Altered allogeneic response in neonatally anti-MHC class I-treated mice.
Neonatal treatment of C3H mice (H-2k) with anti-Kk monoclonal antibodies results in altered cytotoxic responses against allogeneic targets. After 2-3 weeks of antibody treatment, no difference in the number of CD4+8- or CD4-8+ T cells was observed between the antibody- and saline-treated mice. However, antibody-treated mice had a significantly reduced cytotoxic response against various allogeneic major histocompatibility complex (MHC) class I-expressing targets. The strongest reduction was observed in very young mice (up to 2 weeks of age). As the mice got older, the allo MHC-specific responses reached control levels. No significant changes in T-cell receptor (TCR)-V-region usage was observed even in young antibody-treated mice. The results suggest that the reduction in the number of positively selecting elements reduces alloreactivity and most likely also the diversity of TCR-repertoire. However, the reduced alloresponsiveness was not restricted to either allogeneic K- or D-encoded molecules, suggesting that self MHC-D-region encoded molecules can mediate positive selection of T cells able to react against both K and D region-encoded allogeneic MHC class I molecules.
Marusić-Galesić
S
S
Department of Molecular Medicine, Institute Ruder Bosković, Zagreb, Croatia.
Sparbier
K
K
Knezevic
N
N
Walden
P
P
D000818:Animals; D000831:Animals, Newborn; D000911:Antibodies, Monoclonal / Q000008:administration & dosage / Q000494:pharmacology*; D006183:H-2 Antigens / Q000235:genetics / Q000276:immunology*; D007274:Injections, Intraperitoneal; D007518:Isoantibodies / Q000096:biosynthesis* / Q000494:pharmacology*; D007519:Isoantigens / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology
D000911:Antibodies, Monoclonal; D006183:H-2 Antigens; D007518:Isoantibodies; D007519:Isoantigens; D016693:Receptors, Antigen, T-Cell, alpha-beta