Establishment of vasopressin-responsive early proximal tubular cell lines derived from transgenic mice harboring temperature-sensitive simian virus 40 large T-antigen gene.
A little is known about vasopressin receptor in early proximal tubule (S1). The purpose of this study is to establish the vasopressin-responsive S1 cell line derived from transgenic mice harboring temperature-sensitive(ts) simian virus (SV) 40 large T-antigen gene. The cells showed a temperature-sensitive cell growth characteristic of encoding tsSV40. The S1 cells retained a unique morphology specific to proximal tubule. The cells showed the vasopressin-induced increase in intracellular calcium concentration ([Ca2+]i) mediated by both V1a and the putative Vp receptor. Of nineteen clonal cell lines established from the parental cells, three expressed only V1a receptor, and five retained both V1a and Vp receptor. In conclusion, these immortalized S1 cell lines may be useful for studying vasopressin receptor subtypes in S1.
Takeda
M
M
Department of Pharmacology and Toxicology, Kyorin University School of Medicine, Tokyo, Japan.
Hosoyamada
M
M
Shirato
I
I
Obinata
M
M
Suzuki
M
M
Endou
H
H
D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000235:genetics*; D001127:Arginine Vasopressin / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism; D002455:Cell Division / Q000235:genetics; D002461:Cell Line, Transformed; D005814:Genes, Viral; D007687:Kidney Tubules, Proximal / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D051379:Mice; D008822:Mice, Transgenic; D013696:Temperature
D000952:Antigens, Polyomavirus Transforming; D001127:Arginine Vasopressin; D002118:Calcium