title: Resolution of kinase activities during the HeLa cell cycle: identification of kinases with cyclic activities.
abstract: An in situ gel kinase assay was used to resolve kinase activities during the HeLa cell cycle. Three kinase activities changed dramatically during the cell cycle, whereas a number of others remained relatively constant. The former included histone kinases with estimated molecular masses of 46, 87, and 39 kDa. The 46-kDa kinase activity was preferentially expressed at least 10-fold greater in mitotic cells. In contrast, the 87- and 39-kDa kinases were most active early in G1 but their activity declined progressively as cells approached mitosis. The 87- but not the 46- or 39-kDa kinases had autophosphorylation activity. The identities of the kinases are unknown but they may play important roles in regulation of the cell cycle.
authors.affiliation: University of Maryland Biotechnology Institute, University of Maryland School of Medicine, Baltimore 21201, USA.
authors.forename: M J
authors.initials: MJ
authors.lastname: Monteiro
authors.forename: T I
authors.initials: TI
authors.lastname: Mical
mesh_terms: D002453:Cell Cycle / Q000502:physiology*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D006367:HeLa Cells; D006657:Histones / Q000378:metabolism; D006801:Humans; D008938:Mitosis; D008970:Molecular Weight; D010766:Phosphorylation; D006656:Protamine Kinase / Q000737:chemistry / Q000378:metabolism*
chemical_list: D006657:Histones; D006656:Protamine Kinase