Conformational analysis of methylphenidate and its structural relationship to other dopamine reuptake blockers such as CFT.
OBJECTIVE
This work was performed 1) to determine the conformational preferences of the threo and erythro isomers of the dopamine reuptake blocker methylphenidate, 2) to determine the crystal conformation of the threo isomer, 3) to confirm the absolute configuration of the more active threo enantiomer, and 4) to incorporate the compound into a previously determined pharmacophore for dopamine reuptake blockers.


METHODS
A conformational analysis was performed with the MM2-87 program, a crystal of the (-)-threo HCl salt was analyzed by x-ray crystallography, and the global minima of the (+)-threo isomer and the potent dopamine reuptake blocker CFT were superimposed.


RESULTS
In the global minimum of the threo isomer, the carbonyl oxygen of the ester group is oriented toward the ammonium group as was also found in the crystal state. In the erythro isomer, the ester group prefers an extended conformation relative to the piperidine group. The absolute configuration of the biologically active (+)-threo enantiomer was confirmed to be R,R. The atomic sequence from the amine group through the ester group is identical in the active enantiomers of methylphenidate and CFT.


CONCLUSIONS
The dopamine reuptake protein requires a precise orientation of the ammonium and ester groups but allows considerable leeway in the position of the phenyl ring. The pKa of the threo isomer is predicted to be higher than that of the erythro isomer.
Froimowitz
M
M
Pharm-Eco Laboratories, Lexington, Massachusetts 02173, USA.
Patrick
K S
KS
Cody
V
V
D003042:Cocaine / Q000031:analogs & derivatives* / Q000737:chemistry; D018360:Crystallography, X-Ray; D018765:Dopamine Uptake Inhibitors / Q000737:chemistry*; D008774:Methylphenidate / Q000737:chemistry*; D015394:Molecular Structure; D013237:Stereoisomerism
D018765:Dopamine Uptake Inhibitors; D008774:Methylphenidate; C021279:(1R-(exo,exo))-3-(4-fluorophenyl)-8-methyl-8- azabicyclo(3.2.1)octane-2-carboxylic acid, methyl ester; D003042:Cocaine