title: Superoxide anion reduces the ability of myeloperoxidase to damage lipids.
abstract: The aim of this study was to determine whether lipids are damaged by myeloperoxidase (MP) not only with hydrogen peroxide (H2O2), but also with a H2O2-generating system. Using the hypoxanthine-xanthine oxidase-Cu,Zn-superoxide dismutase (HX-XO-SOD) system as an effective H2O2-generating system, we observed a reduction in the ability of MP to produce peroxylipids in the HX-XO-SOD-MP system, compared with the H2O2-MP system. We also noted that MP inhibited the production of monoepoxide by the H2O2-cytochrome c (Cyt c) system. These results suggest that MP plays a role as an absorber of active oxygen species and prevents phagocytes from self-destruction.
authors.affiliation: Department of Forensic Medicine, Faculty of Medicine, University of Tokyo, Bunkyo-ku, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Iwase
authors.forename: T
authors.initials: T
authors.lastname: Takatori
authors.forename: M
authors.initials: M
authors.lastname: Nagao
authors.forename: K
authors.initials: K
authors.lastname: Iwadate
authors.forename: M
authors.initials: M
authors.lastname: Takahashi
authors.forename: M
authors.initials: M
authors.lastname: Nakajima
authors.forename: T
authors.initials: T
authors.lastname: Takahashi
authors.forename: T
authors.initials: T
authors.lastname: Shimizu
authors.forename: K
authors.initials: K
authors.lastname: Takahashi
mesh_terms: D003574:Cytochrome c Group / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D019271:Hypoxanthine; D007042:Hypoxanthines / Q000378:metabolism; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000378:metabolism*; D008054:Lipid Peroxides / Q000378:metabolism*; D009195:Peroxidase / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000378:metabolism; D013481:Superoxides / Q000494:pharmacology*; D014969:Xanthine Oxidase / Q000378:metabolism
chemical_list: D003574:Cytochrome c Group; D007042:Hypoxanthines; D008041:Linoleic Acids; D008054:Lipid Peroxides; D013481:Superoxides; D019271:Hypoxanthine; D019787:Linoleic Acid; D006861:Hydrogen Peroxide; D009195:Peroxidase; D013482:Superoxide Dismutase; D014969:Xanthine Oxidase