title: Involvement of peripheral benzodiazepine receptors in the protection of hematopoietic cells against oxygen radical damage.
abstract: Several putative functions have been attributed to the peripheral benzodiazepine receptor (PBR), but its precise physiologic role has not been elucidated. In the present study, we investigated PBR function by quantifying this receptor in leukocyte subsets from healthy donors and in leukemic blasts from lymphoid and myeloid lineages. Using a monoclonal antibody (MoAb) directed against the human PBR and a quantitative flow cytometric assay, we found that phagocytic cells from healthy donors displayed a higher level of PBRs than lymphocytes or natural killer (NK) cells. Among the lymphoid lineage, thymocytes and IgD-negative B cells expressed the lowest levels. However, because of the wide heterogeneity of PBR levels among 42 acute or chronic lymphoid and myeloid leukemias, it was not possible to assign PBR expression to a stage of maturation or a cell lineage. Although the PBR displayed a mitochondrial subcellular localization, its expression was not correlated with the mitochondrial content, suggesting a modulation of PBR density at the level of the mitochondria. This modulation was confirmed when we studied in detail the PBR expression during T-cell development by both flow cytometry and confocal microscopy. We found that the PBR was expressed with a bimodal profile during T-cell development, identical to the one observed with the proto-oncogene, Bcl-2. The high similarity in the expression of both the PBR and the Bcl-2 proto-oncogene in T-cell and B-cell subsets, their common mitochondrial localization, and the observation of high quantities of PBR in phagocytic cells, which are known to produce high levels of radical oxygen species, suggested that PBRs may participate in an antioxidant pathway. Indeed, a strong correlation was established between the ability of hematopoietic cell lines to resist H202 cytotoxicity and their level of PBR expression. Demonstration of the role of PBR in the protection against H202 was obtained by transfecting JURKAT cells with the human PBR cDNA. Transfected cells exhibited increased resistance to H202 compared with wild-type cells, suggesting that PBR may prevent mitochondria from radical damages and thereby modulate apoptosis in the hematopoietic system.
authors.affiliation: Department of Immunology, Sanofi Recherche, Montpelier, France.
authors.forename: P
authors.initials: P
authors.lastname: Carayon
authors.forename: M
authors.initials: M
authors.lastname: Portier
authors.forename: D
authors.initials: D
authors.lastname: Dussossoy
authors.forename: A
authors.initials: A
authors.lastname: Bord
authors.forename: G
authors.initials: G
authors.lastname: Petitprêtre
authors.forename: X
authors.initials: X
authors.lastname: Canat
authors.forename: G
authors.initials: G
authors.lastname: Le Fur
authors.forename: P
authors.initials: P
authors.lastname: Casellas
mesh_terms: D000328:Adult; D002454:Cell Differentiation; D002675:Child, Preschool; D005260:Female; D006410:Hematopoiesis; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007223:Infant; D007938:Leukemia / Q000473:pathology; D008221:Lymphoid Tissue / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008928:Mitochondria / Q000378:metabolism; D018384:Oxidative Stress; D011166:Porphyrins / Q000096:biosynthesis; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis; D019253:Proto-Oncogene Proteins c-bcl-2; D017382:Reactive Oxygen Species / Q000494:pharmacology*; D011963:Receptors, GABA-A / Q000187:drug effects / Q000502:physiology*; D014407:Tumor Cells, Cultured
chemical_list: C000715470:MAS1 protein, human; D011166:Porphyrins; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D017382:Reactive Oxygen Species; D011963:Receptors, GABA-A; D006861:Hydrogen Peroxide