Endogenous opiates do not modulate LPS-induced alterations in carbohydrate metabolism.
This study aimed to determine the contribution of endogenous opiates to the hormonal and glucose metabolic response to lipopolysaccharide (LPS). Rats were infused with naloxone (NAL) (32 micrograms/h) for 2 h prior to the injection of LPS (100 micrograms/100 g body weight) and hemodynamic, hormonal, and metabolic parameters were determined. NAL produced no detectable alterations in any of the parameters assessed. LPS transiently decreases (26%) mean arterial blood pressure (MABP) and increased plasma glucose concentration (100-130%), glucose rate of appearance (50-100%), and glucose rate of disappearance (50-100%). NAL did not alter the LPS-induced drop in mean arterial blood pressure or the glucose response to LPS. LPS reduced plasma insulin (54%), and increased glucagon (270%), corticosterone (180%), and tumor necrosis factor concentrations in plasma (peak 3200-4600 pg/mL at 90 min), with no modification by NAL pretreatment. These results suggest a lack of involvement of endogenous opiate pathways in the glucose metabolic and hormonal responses to LPS.
Bundz
S
S
Department of Surgery, State University of New York at Stony Brook, 11794-8191, USA.
Molina
P E
PE
Lang
C H
CH
Abumrad
N N
NN
D000818:Animals; D001786:Blood Glucose / Q000187:drug effects / Q000378:metabolism*; D006003:Glycogen / Q000378:metabolism; D006439:Hemodynamics / Q000187:drug effects; D006728:Hormones / Q000378:metabolism; D007773:Lactates / Q000378:metabolism; D019344:Lactic Acid; D008070:Lipopolysaccharides / Q000633:toxicity*; D008297:Male; D009270:Naloxone / Q000494:pharmacology; D009292:Narcotic Antagonists / Q000494:pharmacology; D018847:Opioid Peptides / Q000502:physiology*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013312:Stress, Physiological / Q000378:metabolism* / Q000503:physiopathology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D001786:Blood Glucose; D006728:Hormones; D007773:Lactates; D008070:Lipopolysaccharides; D009292:Narcotic Antagonists; D018847:Opioid Peptides; D014409:Tumor Necrosis Factor-alpha; D019344:Lactic Acid; D009270:Naloxone; D006003:Glycogen