title: An updated two-dimensional gel database of rat liver proteins useful in gene regulation and drug effect studies.
abstract: We have improved upon the reference two-dimensional (2-D) electrophoretic map of rat liver proteins originally published in 1991 (N. L. Anderson et al., Electrophoresis 1991, 12, 907-930). A total of 53 proteins (102 spots) are now identified, many by microsequencing. In most cases, spots cut from wet, Coomassie Blue stained 2-D gels were submitted to internal tryptic digestion [2], and individual peptides, separated by high-performance liquid chromatography (HPLC), were sequenced using a Perkin-Elmer 477A sequenator. Additional spots were identified using specific antibodies.
authors.affiliation: Large Scale Biology Corporation, Rockville, MD 20850-3338, USA. leigh@lsbc,com
authors.forename: N L
authors.initials: NL
authors.lastname: Anderson
authors.forename: R
authors.initials: R
authors.lastname: Esquer-Blasco
authors.forename: J P
authors.initials: JP
authors.lastname: Hofmann
authors.forename: L
authors.initials: L
authors.lastname: Meheus
authors.forename: J
authors.initials: J
authors.lastname: Raymackers
authors.forename: S
authors.initials: S
authors.lastname: Steiner
authors.forename: F
authors.initials: F
authors.lastname: Witzmann
authors.forename: N G
authors.initials: NG
authors.lastname: Anderson
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D015180:Electrophoresis, Gel, Two-Dimensional; D006863:Hydrogen-Ion Concentration; D007256:Information Systems; D007526:Isoelectric Point; D008099:Liver / Q000737:chemistry*; D051379:Mice; D008970:Molecular Weight; D010446:Peptide Fragments / Q000737:chemistry; D011506:Proteins / Q000032:analysis / Q000737:chemistry*; D051381:Rats; D017421:Sequence Analysis; D014357:Trypsin
chemical_list: D010446:Peptide Fragments; D011506:Proteins; D014357:Trypsin