title: cDNA cloning of rat cardiotrophin-1 (CT-1): augmented expression of CT-1 gene in ventricle of genetically hypertensive rats.
abstract: Cardiotrophin-1 (CT-1) is a novel cytokine that was discovered from mouse embryoid bodies by means of expression cloning. It induces hypertrophy in cultured myocytes by activating a gp130 signaling pathway. To investigate the expression of the CT-1 gene in both normal adult and genetically hypertensive rats, we cloned rat CT-1 cDNA. The amino acid sequence is 94% identical to that of mouse CT-1. A significant amount of CT-1 mRNA was expressed in the ventricle of adult rats and also detected in the lung, kidney, liver, skeletal muscle, stomach and urinary bladder. Northern blot analysis revealed that the CT-1 mRNA level is significantly augmented in the ventricle of 12-week-old spontaneously hypertensive rats-stroke prone/Izm at a stage of established hypertension, suggesting a possible relevance of CT-1 to ventricular hypertrophy.
authors.affiliation: Department of Medicine and Clinical Science, Kyoto University Graduate School of Medicine, Sakyo-ku, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Ishikawa
authors.forename: Y
authors.initials: Y
authors.lastname: Saito
authors.forename: Y
authors.initials: Y
authors.lastname: Miyamoto
authors.forename: K
authors.initials: K
authors.lastname: Kuwahara
authors.forename: E
authors.initials: E
authors.lastname: Ogawa
authors.forename: O
authors.initials: O
authors.lastname: Nakagawa
authors.forename: M
authors.initials: M
authors.lastname: Harada
authors.forename: I
authors.initials: I
authors.lastname: Masuda
authors.forename: K
authors.initials: K
authors.lastname: Nakao
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D016887:Cardiopulmonary Resuscitation; D003001:Cloning, Molecular; D016207:Cytokines / Q000096:biosynthesis* / Q000235:genetics; D017931:DNA Primers; D018076:DNA, Complementary; D015870:Gene Expression; D006352:Heart Ventricles; D006973:Hypertension / Q000235:genetics* / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D009206:Myocardium / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D011918:Rats, Inbred SHR; D017208:Rats, Wistar; D011994:Recombinant Proteins / Q000096:biosynthesis; D017386:Sequence Homology, Amino Acid
chemical_list: D016207:Cytokines; D017931:DNA Primers; D018076:DNA, Complementary; D012333:RNA, Messenger; D011994:Recombinant Proteins; C092073:cardiotrophin 1