Human pericardial fluid contains the highest amount of endothelin-1 of all mammalian biologic fluids thus far tested.
Immunoreactive endothelin-1 (ET-1) levels were measured in the pericardial fluid (PF), plasma (PL), and atrial and ventricular tissues of 16 cardiac surgical patients. ET-1 was higher in the PF (73 +/- 11 pg/ml) than in the PL/3.33 +/- 0.51 pg/ml; p < 0.05/. PF/PL ratio ranged from 8 to as high as 200. An inverse relationship was found between the NYHA stage and ET-1 concentrations in PF, whereas ET-1 in PL did not have any relation to the NYHA stage of the disease. In HPLC analysis, the total immunoreactive ET-1 in PF from five different patients co-eluted with the human ET-1 standard. The results indicate that ET-1 concentrations in human PF can be several-fold those in the PL. The source and function of the ET-1 in PF requires further investigation.
Horkay
F
F
Department of Cardiovascular Surgery, Semmelweis Medical University, Budapest, Hungary.
Laine
M
M
Szokodi
I
I
Leppäluoto
J
J
Vuolteenaho
O
O
Ruskoaho
H
H
Juhász-Nagy
A
A
Tóth
M
M
D001826:Body Fluids / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D016232:Endothelins / Q000032:analysis* / Q000097:blood; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010496:Pericardium / Q000737:chemistry*
D016232:Endothelins