Immunoreactivity for apolipoprotein A-IV in tanycytes and astrocytes of rat brain.
Immunoperoxidase and immunofluorescence procedures were used to visualize polyclonal antiserum binding to apolipoprotein (apo) A-IV in rat brain. With both methods, tanycytes and astrocytes were labeled throughout both white and gray matter. Within the cells, the labeling was granular and it was confined to the perinuclear zone and proximal regions of the processes. The labeling was abolished by absorption of the primary antiserum with purified apo A-IV but not by absorption with apo E. These results suggest either that apo A-IV is synthesized by astrocytes, or that apolipoprotein that is synthesized in the small intestine or liver is selectively taken up and stored by the astrocytes.
Fukagawa
K
K
Department of Physiology, Louisiana State University Medical Center, Shreveport 71130, USA.
Knight
D S
DS
Hamilton
K A
KA
Tso
P
P
D000818:Animals; D001054:Apolipoproteins A / Q000032:analysis* / Q000276:immunology; D001253:Astrocytes / Q000737:chemistry*; D015153:Blotting, Western; D001921:Brain / Q000166:cytology*; D001923:Brain Chemistry; D002552:Cerebral Ventricles / Q000737:chemistry / Q000166:cytology*; D019085:Fluorescent Antibody Technique, Direct; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D007124:Immunoenzyme Techniques; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
D001054:Apolipoproteins A; D005904:Glial Fibrillary Acidic Protein; C022205:apolipoprotein A-IV