Skin allograft rejection in CD28-deficient mice.
Costimulatory interactions between CD28 and the B7 family have been shown to augment T cell responses in general. To further assess the importance of the costimulatory signals generated through CD28, the allogeneic response was examined in CD28-deficient mice. T cells from CD28-deficient mice showed reduced proliferation and cytokine production in response to allogeneic stimulator cells in vitro. However, CD28-deficient T cells developed cytotoxic activity against allogeneic target cells in vitro and efficiently rejected skin allografts in vivo. These results suggest that the costimulatory signals through CD28 are not essential for the induction of alloreactive effector functions in vitro and in vivo.
Kawai
K
K
Ontario Cancer Institute, Department of Medical Biophysics, University of Toronto, Canada.
Shahinian
A
A
Mak
T W
TW
Ohashi
P S
PS
D000818:Animals; D019138:Antibodies, Blocking / Q000494:pharmacology; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D018106:CD28 Antigens / Q000235:genetics / Q000378:metabolism*; D003602:Cytotoxicity, Immunologic; D005260:Female; D006084:Graft Rejection / Q000276:immunology*; D066298:In Vitro Techniques; D007376:Interleukin-2 / Q000096:biosynthesis; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D018345:Mice, Knockout; D016038:Skin Transplantation / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology; D014184:Transplantation, Homologous
D019138:Antibodies, Blocking; D000911:Antibodies, Monoclonal; D018106:CD28 Antigens; D007376:Interleukin-2