title: Conversion studies with substrate analogues of toluene in a sulfate-reducing bacterium, strain Tol2.
abstract: Anaerobic toluene oxidation by the sulfate-reducing bacterium, strain Tol2 (proposed name Desulfobacula toluolica) was specifically inhibited by benzyl alcohol when added at concentrations around 500 microM. Benzyl alcohol added at lower, non-inhibitory concentrations (around 5 microM) was not oxidized by active cells pregrown on toluene, indicating that the alcohol is not a free intermediate of toluene metabolism in the sulfate reducer. Conversion of p-xylene in toluene-metabolizing cells to p-methylbenzoate as dead-end product suggests that the sulfate reducer, like denitrifiers, initiates toluene oxidation at the methyl group.
authors.affiliation: Max-Planck-Institut für Marine Mikrobiologie, Fahrenheitstrasse 1, D-28359 Bremen, Germany.
authors.forename: R
authors.initials: R
authors.lastname: Rabus
authors.forename: F
authors.initials: F
authors.lastname: Widdel
mesh_terms: D001421:Bacteria, Anaerobic / Q000378:metabolism*; D019905:Benzyl Alcohol; D001592:Benzyl Alcohols / Q000378:metabolism; D001673:Biodegradation, Environmental; D010084:Oxidation-Reduction; D013431:Sulfates / Q000378:metabolism*; D014050:Toluene / Q000378:metabolism*
chemical_list: D001592:Benzyl Alcohols; D013431:Sulfates; D014050:Toluene; D019905:Benzyl Alcohol