Inhibitory effect of some oxygenated stigmastane-type sterols on 12-O-tetradecanoylphorbol-13-acetate-induced inflammation in mice.
The oxygenated stigmastane-type sterols stigmastane-3 beta, 6 alpha-diol, stigmastane-3 beta, 6 beta-diol, 7 alpha-hydroxysitosterol and its diacetyl derivative, 7 beta-hydroxysitosterol and its diacetyl derivative, 7-oxositosterol, 4 beta-hydroxysitosterol, and stigmast-4-ene-3 beta, 6 beta-diol were evaluated with respect to their anti-inflammatory activity against 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced inflammation in mice. All of the sterols, with the exception of 7 alpha-hydroxysitosterol and its diacetyl derivative, were found to possess marked inhibitory activity. The 50% inhibitory dose of these compounds for TPA-inflammation (1 microgram) was 0.5--1.0 mg/ear.
Kimura
Y
Y
College of Pharmacy, Nihon University, Chiba, Japan.
Yasukawa
K
K
Takido
M
M
Akihisa
T
T
Tamura
T
T
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D004431:Ear, External; D004487:Edema / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control; D005260:Female; D007249:Inflammation / Q000139:chemically induced / Q000517:prevention & control*; D051379:Mice; D008813:Mice, Inbred ICR; D010840:Phytosterols / Q000494:pharmacology*; D013755:Tetradecanoylphorbol Acetate / Q000037:antagonists & inhibitors* / Q000633:toxicity
D000894:Anti-Inflammatory Agents, Non-Steroidal; D010840:Phytosterols; D013755:Tetradecanoylphorbol Acetate