title: Effect of pentoxifylline added to freezing media on subsequent post-thaw hypoosmotic swelling test and other semen parameters.
abstract: This study evaluated the effects of pentoxifylline (PTX) on post-thaw semen parameters as well as the hypoosmotic swelling (HOS) test. Fourteen samples were evaluated for volume, count, motility, % grade A sperm, and HOS test. Two aliquots were frozen, one in freezing medium and the other in a 3 mM solution of PTX and freezing medium. Both groups were frozen in liquid nitrogen vapors for 30 min. Thawing was performed at 37 degrees C for 15 min, followed by a wash with 2 parts 0.5% HSA/MHTF to 1 part sample. Pellets were resuspended in 0. MHTF and then evaluated as described above. In addition, motility was evaluated 2 h post-thaw. Following freeze-thaw, the mean motile densities were similar (17.5 x 10 motile/mL vs. 20.4 x 10(6) motile/mL for PTX and control, respectively). Two hours post-thaw, the PTX group had a mean sperm motility of 31.3% vs. 37.7% for the control group (p > .05). There were no significant differences in % grade A sperm in PTX (13.0%) vs. control (12.0%). Similarly, HOS scores did not improve following cryopreservation (43.0% and 50.6% for PTX and control, respectively). Thus, no improvement was found by freezing sperm with PTX.
authors.affiliation: University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School at Camden, Cooper Hospital, USA.
authors.forename: D J
authors.initials: DJ
authors.lastname: Check
authors.forename: D
authors.initials: D
authors.lastname: Kiefer
authors.forename: D
authors.initials: D
authors.lastname: Katsoff
authors.forename: J H
authors.initials: JH
authors.lastname: Check
mesh_terms: D000704:Analysis of Variance; D015925:Cryopreservation / Q000379:methods; D006801:Humans; D008297:Male; D009995:Osmosis / Q000187:drug effects / Q000502:physiology; D010431:Pentoxifylline / Q000494:pharmacology*; D012661:Semen / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D012662:Semen Preservation / Q000379:methods; D013081:Sperm Motility / Q000187:drug effects / Q000502:physiology; D013094:Spermatozoa / Q000187:drug effects* / Q000502:physiology; D014665:Vasodilator Agents / Q000494:pharmacology*
chemical_list: D014665:Vasodilator Agents; D010431:Pentoxifylline