N-ras and p53 gene mutations are very rare events in multiple myeloma.
The frequencies of p53 and N-ras gene mutations were examined in multiple myeloma. DNA samples of 45 cases of multiple myeloma were obtained from stored bone marrow smears. All samples were analyzed for the N-ras gene at codon 12 by the allele specific restriction analysis method and at codon 61 by direct sequencing. DNA was screened for mutations by single-strand conformation polymorphism analysis for the p53 gene in exons 5 to 8. DNA fragments showing an altered electrophoretic pattern were further studied by direct sequencing to confirm the mutations. Ras mutations were found in two cases at codon 61 (4.4%). A point mutation of the p53 gene was detected in one of 45 cases (2.2%). These 3 cases with mutations were in an advanced stage. In conclusion, N-ras and p53 gene mutations may occur at a late stage of multiple myeloma, but the frequencies of the mutations are very low.
Yasuga
Y
Y
First Department of Internal Medicine, Tokyo Medical and Dental University, Japan.
Hirosawa
S
S
Yamamoto
K
K
Tomiyama
J
J
Nagata
K
K
Aokia
N
N
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001483:Base Sequence; D001853:Bone Marrow / Q000473:pathology; D003062:Codon / Q000235:genetics; D004252:DNA Mutational Analysis; D004273:DNA, Neoplasm / Q000235:genetics*; D005260:Female; D016158:Genes, p53; D011905:Genes, ras; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009101:Multiple Myeloma / Q000235:genetics* / Q000473:pathology; D009154:Mutation; D009194:Myeloma Proteins / Q000032:analysis; D016133:Polymerase Chain Reaction
D003062:Codon; D004273:DNA, Neoplasm; D009194:Myeloma Proteins