title: Lens-preferred activity of the -1809/+46 mouse alpha A-crystallin promoter in stably integrated chromatin.
abstract: The alpha A-crystallin gene is expressed in a highly lens preferred manner. Here we show that the mouse alpha A-crystallin -1809/+46 promoter fragment displays lens-preferred activity in transgenic mice and in stably transfected lens cells. These findings are in contrast to the lack of activity of this promoter previously reported in transiently transfected lens cells. Our current findings suggest that the -1809/+46 mouse alpha A-crystallin promoter functions in a lens preferred manner when stably integrated into chromatin.
authors.affiliation: Laboratory of Molecular and Developmental Biology, National Eye Institute, National Institutes of Health, Bethesda, MD 20892, USA.
authors.forename: C M
authors.initials: CM
authors.lastname: Sax
authors.forename: J G
authors.initials: JG
authors.lastname: IIagan
authors.forename: J I
authors.initials: JI
authors.lastname: Haynes
mesh_terms: D000818:Animals; D002460:Cell Line; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D002843:Chromatin / Q000235:genetics; D003459:Crystallins / Q000235:genetics*; D005260:Female; D015870:Gene Expression; D007908:Lens, Crystalline / Q000378:metabolism*; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D011401:Promoter Regions, Genetic; D014018:Tissue Distribution; D014162:Transfection
chemical_list: D002843:Chromatin; D003459:Crystallins; D015500:Chloramphenicol O-Acetyltransferase