title: High pressure represses expression of the malB operon in Escherichia coli.
abstract: The formation of plaques by lambda phage in Escherichia coli was prevented by elevated hydrostatic pressure; phage plaques were not detected at 30 MPa. Furthermore, using promoter fragments derived from the malB operon, we showed that gene expression initiated from both promoters (malK-lamB and malEFG) was repressed by elevated hydrostatic pressure. Our findings suggest that high pressure affects gene expression directed by the malB regulatory interval, and this may cause a decrease in the quantities of lambda receptor protein, LamB.
authors.affiliation: DEEPSTAR group, Japan Marine Science and Technology Center, Yokosuka, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Sato
authors.forename: Y
authors.initials: Y
authors.lastname: Nakamura
authors.forename: K K
authors.initials: KK
authors.lastname: Nakashima
authors.forename: C
authors.initials: C
authors.lastname: Kato
authors.forename: K
authors.initials: K
authors.lastname: Horikoshi
mesh_terms: D001274:Atmospheric Pressure; D001435:Bacteriophages / Q000254:growth & development; D004926:Escherichia coli / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial / Q000502:physiology*; D017930:Genes, Reporter / Q000502:physiology; D009876:Operon / Q000502:physiology*; D011401:Promoter Regions, Genetic