Inhibition of catalase activity in vitro by diesel exhaust particles.
The effect of diesel exhaust particles (DEP) on the activity of catalase, an intracellular antioxidant, was investigated because H2O2 is a cytotoxic oxidant, and catalase released from alveolar cells is an important antioxidant in the epithelial lining fluid in the lung. DEP inhibited the activity of bovine liver catalase dose-dependently, to 25-30% of its original value. The inhibition of catalase by DEP was observed only in the presence of anions such as Cl-,Br-, or thiocyanate. Other anions, such as CH3COO- or SO4-, and cations such as K+, Na+, Mg2+, or Fe2+, did not affect the activity of catalase, even in the presence of DEP extract. Catalase from guinea pig alveolar cells and catalase from red blood cells were also inhibited by DEP extracts, as was catalase from bovine liver. These results suggest that DEP taken up in the lung and located on alveolar spaces might cause cell injury by inhibiting the activity of catalase in epithelial lining fluid, enhancing the toxicity of H2O2 generated from cells in addition to that of O2- generated by the chemical reaction of DEP with oxygen.
Mori
Y
Y
Department of Microbiology, Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido, Japan.
Murakami
S
S
Sagae
T
T
Hayashi
H
H
Sakata
M
M
Sagai
M
M
Kumagai
Y
Y
D000818:Animals; D002374:Catalase / Q000037:antagonists & inhibitors*; D006168:Guinea Pigs; D006861:Hydrogen Peroxide / Q000633:toxicity; D008297:Male; D012965:Sodium Chloride / Q000494:pharmacology; D001335:Vehicle Emissions / Q000009:adverse effects*
D001335:Vehicle Emissions; D012965:Sodium Chloride; D006861:Hydrogen Peroxide; D002374:Catalase