Modified procedure for recovery of Giardia cysts from diverse water sources.
A compact battery-operated, portable field filtration apparatus was developed for efficient recovery of parasites, including Giardia lamblia cysts, from large volumes of water. In conjunction with modified filter elution and processing procedures, the filtration device was evaluated in field trials. To monitor performance of the procedure, a known number of Giardia lambia cysts were added during filtrations through a control injection assembly. This allowed calculation of cyst recovery at each test site, thereby allowing comparison of percentage recoveries from many diverse water sources. This apparatus and modified method now provide the tools to investigate further extraction and purification techniques to improve overall yield of Giardia lamblia, other parasites and potentially other organisms such as bacteria and viruses.
Bielec
L
L
Hamilton Public Health Laboratory, Ontario Ministry of Health, Hamilton, Canada.
Boisvert
T C
TC
Jackson
S G
SG
D000818:Animals; D005069:Evaluation Studies as Topic; D005374:Filtration / Q000295:instrumentation / Q000379:methods; D016829:Giardia lamblia / Q000302:isolation & purification*; D010274:Parasitology / Q000295:instrumentation / Q000379:methods*; D014867:Water / Q000469:parasitology*
D014867:Water