Odors increase Fos in olfactory bulb neurons including dopaminergic cells.
Neuronal activity may lead to long lasting changes in cell phenotype through induction of genes such as c-fos which encode transcriptional regulatory factors. Odor-activated olfactory bulb cells exhibit increases in c-fos mRNA expression. The present study examined whether odor stimulation of awake rats also leads to increases in Fos protein in these cells. The phenotype of Fos-immunoreactive cells was partially characterized using double-immunoperoxidase staining. Odor exposure increased Fos-immunoreactivity (IR) in specific sets of olfactory bulb neurons. Fos-IR was not co-localized with IR for glial fibrillary acidic protein, but was co-localized with tyrosine hydroxylase (TH)-IR in a subpopulation of dopaminergic neurons, suggesting that bulbar TH expression may be regulated in part by a Fos mechanism.
Guthrie
K M
KM
Department of Anatomy and Neurobiology, University of California, Irvine 92717, USA.
Gall
C M
CM
D000818:Animals; D001253:Astrocytes / Q000737:chemistry; D004298:Dopamine / Q000502:physiology*; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism*; D009812:Odorants; D009830:Olfactory Bulb / Q000166:cytology / Q000378:metabolism*; D010641:Phenotype; D016760:Proto-Oncogene Proteins c-fos / Q000032:analysis / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D014158:Transcription, Genetic; D014446:Tyrosine 3-Monooxygenase / Q000032:analysis
D005904:Glial Fibrillary Acidic Protein; D009419:Nerve Tissue Proteins; D016760:Proto-Oncogene Proteins c-fos; D014446:Tyrosine 3-Monooxygenase; D004298:Dopamine