title: Faster and cheaper PCR on a standard thermocycler.
abstract: The PCR conditions have been optimized to make the process faster and more economical. When short DNA fragments are to be amplified, the time of denaturation, annealing and extension steps can be as short as 1 s each, and the yield of PCR product is still high, sufficient for many types of analysis. The PCR can be done even in a reaction volume as low as 1 microliter. The recommended volume, 2.5 microliters or 5 microliters, allows significant savings in the laboratory budget especially for laboratories which use PCR frequently and on a large scale.
authors.affiliation: Laboratory of Cancer Genetics, Polish Academy of Sciences, Poznań, Poland.
authors.forename: K
authors.initials: K
authors.lastname: Sobczak
authors.forename: P
authors.initials: P
authors.lastname: Kozłowski
authors.forename: W J
authors.initials: WJ
authors.lastname: Krzyzosiak
mesh_terms: D001483:Base Sequence; D001943:Breast Neoplasms / Q000235:genetics; D003365:Costs and Cost Analysis; D017931:DNA Primers / Q000235:genetics; D004273:DNA, Neoplasm / Q000235:genetics; D005260:Female; D016158:Genes, p53; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation; D016133:Polymerase Chain Reaction / Q000191:economics / Q000379:methods*; D018807:Polymorphism, Single-Stranded Conformational; D013997:Time Factors
chemical_list: D017931:DNA Primers; D004273:DNA, Neoplasm