Detection of Brucella abortus antigens by immunoperoxidase histochemical staining of lochia smears.
An immunoperoxidase histochemical staining technique for the detection of Brucella abortus organisms in lochia of brucellosis-infected cows was described. In comparison with the standard culture technique, the former method resulted in 36% false positive and 2.3% false negative reactions, which represents a sensitivity of 82% and a specificity of 94%. False positive reactions are presumably the result of organisms which succumbed during transit. The feasibility of the test in the confirmation of brucellosis is discussed.
Alberts
J N
JN
Veterinary Laboratory, Kroonstad, South Africa.
Erasmus
J A
JA
D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis*; D002003:Brucella abortus / Q000276:immunology / Q000302:isolation & purification*; D002007:Brucellosis, Bovine / Q000175:diagnosis* / Q000382:microbiology; D002417:Cattle; D007124:Immunoenzyme Techniques; D013019:South Africa
D000942:Antigens, Bacterial