title: Serum levels of interleukin 4 and soluble CD23 in children with allergic disorders.
abstract: In order to clarify the clinical significance of serum interleukin 4 (IL-4) levels, we measured serum IL-4 concentrations in allergic and non-allergic children using a highly sensitive sandwich ELISA. The limit of detection of the assay was 0.15 pg/ml in serum samples. Serum IL-4 was detected in 96.3% (53/55) of non-allergic controls, in 92.9% (183/197) of allergic children, in 70% (7/10) of cord blood samples and in 86.7% (26/30) of neonates. The IL-4 levels in sera from non-allergic controls were relatively constant during the ages examined and all samples were under 1.5 pg/ml. In allergic children, the serum levels of IL-4 were significantly elevated, particularly at age 13-24 months. The serum levels of IL-4 did not differ in children with different clinical manifestations of allergy, such as bronchial asthma, and atopic dermatitis. The serum level of soluble CD23 (sCD23) showed an age-dependent change in allergic and non-allergic children and was significantly higher in allergic than in non-allergic infants aged 7 to 12 months, but not in other age groups. There was no significant correlation among serum levels of IL-4, sCD23 and IgE.
  
  
  CONCLUSIONS
  It is suggested that the measurement of serum IL-4 and sCD23 is helpful in the examination of allergic patients in infancy and early childhood, but neither the serum level of IL-4 nor sCD23 directly reflects in vivo IgE production.
authors.affiliation: Department of Paediatrics, Minami-Kyoto National Hospital, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Ohshima
authors.forename: K
authors.initials: K
authors.lastname: Katamura
authors.forename: M
authors.initials: M
authors.lastname: Miura
authors.forename: H
authors.initials: H
authors.lastname: Mikawa
authors.forename: M
authors.initials: M
authors.lastname: Mayumi
mesh_terms: D001249:Asthma / Q000097:blood; D002648:Child; D002675:Child, Preschool; D003233:Conjunctivitis, Allergic / Q000097:blood; D003876:Dermatitis, Atopic / Q000097:blood; D005260:Female; D005512:Food Hypersensitivity / Q000097:blood; D006801:Humans; D006967:Hypersensitivity / Q000097:blood*; D007223:Infant; D007231:Infant, Newborn; D015847:Interleukin-4 / Q000097:blood*; D008297:Male; D017455:Receptors, IgE / Q000032:analysis*; D012220:Rhinitis / Q000097:blood
chemical_list: D017455:Receptors, IgE; D015847:Interleukin-4