title: Relationships between denture base resin cytotoxicity and cell lipid metabolism.
abstract: Substances that elute from denture base resins may inhibit cell growth and disrupt various metabolic processes. This study investigated the effects on cell lipid metabolism of eluates from several denture base resins. Cultured oral epithelial cells were exposed in vitro to eluates of discs made from several denture base resins. Lipid metabolism of the cells was measured using isotopic labeling with 14C-acetate. Results demonstrated that the metabolism of several lipid classes found mainly in the cell membrane was altered by the resin eluates. Eluate from one resin caused the appearance of two previously unrecognized classes of lipids. The alterations of the cell lipids and the presence of the previously unrecognized lipids may be the basis for some clinically evident cytotoxic and allergic reactions.
authors.affiliation: Department of Oral Biology, School of Dentistry, Medical College of Georgia, Augusta, Georgia, USA.
authors.forename: G S
authors.initials: GS
authors.lastname: Schuster
authors.forename: C A
authors.initials: CA
authors.lastname: Lefebvre
authors.forename: T R
authors.initials: TR
authors.lastname: Dirksen
authors.forename: K L
authors.initials: KL
authors.lastname: Knoernschild
authors.forename: G B
authors.initials: GB
authors.lastname: Caughman
mesh_terms: D000180:Acrylic Resins / Q000633:toxicity; D000704:Analysis of Variance; D000818:Animals; D002462:Cell Membrane / Q000187:drug effects; D002478:Cells, Cultured; D002610:Cheek; D002784:Cholesterol / Q000378:metabolism; D002788:Cholesterol Esters / Q000378:metabolism; D006224:Cricetinae; D003819:Denture Bases / Q000009:adverse effects*; D004847:Epithelial Cells; D004848:Epithelium / Q000187:drug effects / Q000378:metabolism; D050356:Lipid Metabolism; D008055:Lipids / Q000032:analysis; D008563:Membrane Lipids / Q000378:metabolism; D009061:Mouth Mucosa / Q000166:cytology / Q000187:drug effects; D010713:Phosphatidylcholines / Q000378:metabolism; D012117:Resins, Synthetic / Q000737:chemistry / Q000633:toxicity*; D013261:Sterols / Q000378:metabolism; D014280:Triglycerides / Q000378:metabolism
chemical_list: D000180:Acrylic Resins; C078088:Astron LC; D002788:Cholesterol Esters; D008055:Lipids; D008563:Membrane Lipids; D010713:Phosphatidylcholines; D012117:Resins, Synthetic; D013261:Sterols; D014280:Triglycerides; C060386:Triad resin; C040291:Lucitone; D002784:Cholesterol