A conformational model for cyclic beta-(1-->2)-linked glucans based on NMR analysis of the beta-glucans produced by Xanthomonas campestris.
A cyclic hexadecaglucoside containing 15 beta-(1-->2)-linkages and one alpha-(1-->6)-linkage (A. Amemura and J. Cabrera-Crespo, J. Gen. Microbiol. 132 (1986) 2443-2452) was purified from cultures of Xanthomonas campestris. The homogeneity of this glucan preparation facilitated the complete assignment of its 1H NMR spectrum and the assignment of all of the C-1 and C-2 resonances in its 13C NMR spectrum to specific residues within the glucan. The resonances (i.e., H-1, H-2, C-1 and C-2) that are closely associated with the beta-(1-->2) glucosidic bonds of this glucan are dispersed over a relatively broad chemical shift range. This chemical shift dispersion is attributed to the differences in the time-averaged geometry of individual beta-(1-->2)-linked glycosidic bonds in this glucan and is consistent with the hypothesis that these glycosidic bonds have less conformational freedom than do the glycosidic bonds in a linear beta-(1-->2)-linked glucan. The chemical shifts of H-1, H-2, C-1, and C-2 exhibit an alternating pattern when plotted as a function of their locations within the macrocyclic ring, suggesting that the glycosidic bond geometry also alternates in the glucan. A new conformational model for cyclic beta-(1-->2)-linked glucans was developed on the basis of these observations. This model is consistent with the observed spectroscopic features of all cyclic beta-(1-->2)-linked glucans known to be produced by Gram-negative bacteria.
York
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Complex Carbohydrate Research Center, University of Georgia, Athens 30602-4712, USA.
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D003505:Cyclodextrins / Q000737:chemistry*; D006090:Gram-Negative Bacteria / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000302:isolation & purification; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D016959:Xanthomonas campestris / Q000737:chemistry*; D047391:alpha-Cyclodextrins
D003505:Cyclodextrins; D011135:Polysaccharides, Bacterial; D047391:alpha-Cyclodextrins; C098291:alpha-cyclosophorohexadecaose