A lectin-like peptide isolated from the venom of the Chinese bird spider Selenocosmia huwena.
A peptide with haemagglutination activity was isolated from the venom of the Chinese bird spider Selenocosmia huwena by means of ion-exchange and reverse-phase high-performance liquid chromatography. This peptide, named SHLP-I, agglutinates human and mice erythrocytes at a minimum concentration of 125 micrograms/ml and 31 micrograms/ml, respectively. It consists of 32 amino acid residues including 3 Trp and 6 Cys residues, the latter of which form three disulfide bounds. The complete amino acid sequence was determined. The N-terminal and C-terminal residues were Gly and Trp, respectively. SHLP-I shows homology with a fragment of great nettle lectin and with huwentoxin-I from the venom of the same spider.
Liang
S P
SP
Department of Biology, Human Normal University, Changsha, P.R. China.
Pan
X
X
D000595:Amino Acid Sequence; D000818:Animals; D005260:Female; D006386:Hemagglutination Tests; D006388:Hemagglutinins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D019476:Insect Proteins; D037102:Lectins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D051379:Mice; D008969:Molecular Sequence Data; D013111:Spider Venoms / Q000737:chemistry* / Q000494:pharmacology
D006388:Hemagglutinins; D019476:Insect Proteins; D037102:Lectins; D013111:Spider Venoms; C098229:lectin-like peptide-I, Selenocosmia huwena