Comparison of three methods for competitive binding of monoclonal antibodies. The localization of antigenic sites for monoclonal antibodies on Panulirus interruptus hemocyanin.
The competitive binding of a panel of monoclonal antibodies against hemocyanin of Panulirus interruptus hemocyanin was investigated with three different methods. A competitive-binding immunoassay method was more successful in the determination of ternary complexes than gel electrophoresis and gel filtration. The latter two methods could only be applied with antibodies possessing a higher affinity. Eleven monoclonal antibodies were assigned to groups on the basis of their interactions with five antigenic regions.
Perton
F G
FG
Biochemisch Laboratorium, Rijksuniversiteit Groningen, Netherlands.
Dijkema
J H
JH
Smilda
T
T
Erik van Uffelen
B
B
Beintema
J J
JJ
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D001666:Binding Sites, Antibody; D001667:Binding, Competitive; D002850:Chromatography, Gel; D003445:Crustacea / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D018604:Epitope Mapping / Q000379:methods*; D006433:Hemocyanins / Q000737:chemistry / Q000276:immunology*
D000911:Antibodies, Monoclonal; D006433:Hemocyanins