cDNA cloning of porcine granulocyte-macrophage colony-stimulating factor.
Porcine granulocyte-macrophage colony-stimulating factor (GM-CSF) cDNA was cloned by using polymerase chain reaction (PCR) and sequenced. A coding sequence of the porcine GM-CSF precursor protein, including the signal peptide sequence and stop codon, is 435 bp in length. The identities of the porcine GM-CSF coding sequence when compared to ovine, bovine, human and murine sequences were 89, 86, 83 and 70% at the nucleotide level, and 80, 74, 73, and 56% at the amino acid level. The hydrophobicity profiles, putative glycosylation sites and positions of cysteine residues were highly conserved in porcine, ovine, bovine and human GM-CSF but not murine. This is the first report of the porcine GM-CSF cDNA cloning and sequence.
Inumaru
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Department of Immunology, BBSRC Institute for Animal Health, Pirbright Laboratory, Woking, Surrey, UK.
Takamatsu
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H
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D003001:Cloning, Molecular / Q000379:methods; D018076:DNA, Complementary / Q000235:genetics; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000235:genetics*; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D013552:Swine
D018076:DNA, Complementary; D016178:Granulocyte-Macrophage Colony-Stimulating Factor