SGP28, a novel matrix glycoprotein in specific granules of human neutrophils with similarity to a human testis-specific gene product and a rodent sperm-coating glycoprotein.
A novel 28 kDa glycoprotein was purified from exocytosed material from human neutrophils and its primary structure partially determined. Degenerate oligonucleotide primers were used to amplify cDNA clones from a human bone marrow cDNA library. The deduced 245 amino acid sequence of the 2124 bp full-length cDNA showed high degrees of similarity to the deduced sequences of human gene TPX-1 and of sperm-coating glycoprotein from rat and mouse. Subcellular fractionation of human neutrophils indicated that the protein is localized in specific granules. The protein was named SGP28 (specific granule protein of 28 kDa).
Kjeldsen
L
L
Granulocyte Research Laboratory, Department of Hematology, The Finsen Center, Rigshospitalet, Denmark.
Cowland
J B
JB
Johnsen
A H
AH
Borregaard
N
N
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015815:Cell Adhesion Molecules; D002458:Cell Fractionation; D003594:Cytoplasmic Granules / Q000737:chemistry*; D018076:DNA, Complementary / Q000737:chemistry; D005089:Exocytosis; D006023:Glycoproteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D009504:Neutrophils / Q000648:ultrastructure*; D016133:Polymerase Chain Reaction; D051381:Rats; D012471:Salivary Proteins and Peptides; D029589:Seminal Plasma Proteins; D017385:Sequence Homology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
C062790:CRISP2 protein, rat; C489926:CRISP3 protein, human; D015815:Cell Adhesion Molecules; C489927:Crisp3 protein, mouse; D018076:DNA, Complementary; D006023:Glycoproteins; D012471:Salivary Proteins and Peptides; D029589:Seminal Plasma Proteins; C098596:sperm-coating glycoprotein, rat; D013755:Tetradecanoylphorbol Acetate