Modifications of neurofilament proteins by possible metabolites of allyl chloride in vitro.
Chronic exposure to allyl chloride (ALL) is known to produce a central-peripheral distal axonopathy. In relation to the mechanism(s), the present study was conducted to examine the abilities of ALL and its putative metabolites, i.e., epichlorohydrin, glycerol alpha-monochlorohydrin, allyl alcohol and acrolein to cross-link proteins in vitro. Neurofilament-riched cytoskeletal proteins (1mg/ml) and ovalbumin (10mg/ml) were incubated with 160 mM tested chemicals except for acrolein at 0.5 mM and 1 mM. Time-dependent studies by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) revealed that not only ALL, but also acrolein and epichlorohydrin exerted chemical modifications on axonal cytoskeletal proteins; while only acrolein-treated ovalbumin could manifest evidence of polymerization of the protein. Immunoblotting of PAGE-separated proteins confirmed that the high molecular weight proteins on the top of SDS-PAGEs were NF antigen-contained covalent cross-linked materials.
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Department of Public Health, Kumamoto University School of Medicine, Japan.
Nagano
M
M
Yamamoto
H
H
Miyamoto
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E
Futatsuka
M
M
D000433:1-Propanol / Q000378:metabolism / Q000633:toxicity; D000171:Acrolein / Q000378:metabolism / Q000633:toxicity; D000498:Allyl Compounds / Q000008:administration & dosage / Q000378:metabolism / Q000633:toxicity*; D000818:Animals; D002273:Carcinogens / Q000008:administration & dosage / Q000633:toxicity*; D003272:Contraceptive Agents, Male / Q000008:administration & dosage / Q000633:toxicity*; D004591:Electrophoresis, Polyacrylamide Gel; D004811:Epichlorohydrin / Q000378:metabolism / Q000633:toxicity; D015151:Immunoblotting; D008297:Male; D008970:Molecular Weight; D016900:Neurofilament Proteins / Q000187:drug effects* / Q000378:metabolism; D010047:Ovalbumin / Q000378:metabolism; D020005:Propanols; D051381:Rats; D017208:Rats, Wistar; D013116:Spinal Cord / Q000378:metabolism; D000517:alpha-Chlorohydrin / Q000378:metabolism / Q000633:toxicity
D000498:Allyl Compounds; D002273:Carcinogens; D003272:Contraceptive Agents, Male; D016900:Neurofilament Proteins; D020005:Propanols; D004811:Epichlorohydrin; C006463:allyl alcohol; D000171:Acrolein; D010047:Ovalbumin; D000517:alpha-Chlorohydrin; D000433:1-Propanol; C023698:allyl chloride