title: Vitamin K2 inhibits osteoclastic bone resorption by inducing osteoclast apoptosis.
abstract: In contrast to vitamin K1(VK1), vitamin K2(VK2) inhibited osteoclastic bone resorption by unfractionated bone cells and isolated osteoclasts. To investigate the mechanism of inhibition of osteoclastic bone resorption by VK2, we examined the effect of this vitamin on osteoclast apoptosis using a DNA-binding fluorescent dye, Hoechst 33258. In unfractionated bone cells and isolated osteoclasts on dentin slices, we first demonstrated that VK2 induced osteoclast apoptosis, but VK1 did not. Moreover, cycloheximide inhibited VK2-induced osteoclast apoptosis. These results suggest the possibility that VK2 inhibits osteoclastic bone resorption by targeting osteoclasts to undergo apoptosis, which leads to cell death.
authors.affiliation: Department of Orthodontics, School of Dentistry at Niigata, Nippon Dental University, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Kameda
authors.forename: K
authors.initials: K
authors.lastname: Miyazawa
authors.forename: Y
authors.initials: Y
authors.lastname: Mori
authors.forename: T
authors.initials: T
authors.lastname: Yuasa
authors.forename: M
authors.initials: M
authors.lastname: Shiokawa
authors.forename: Y
authors.initials: Y
authors.lastname: Nakamaru
authors.forename: H
authors.initials: H
authors.lastname: Mano
authors.forename: Y
authors.initials: Y
authors.lastname: Hakeda
authors.forename: A
authors.initials: A
authors.lastname: Kameda
authors.forename: M
authors.initials: M
authors.lastname: Kumegawa
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D001862:Bone Resorption; D002478:Cells, Cultured; D003804:Dentin; D007700:Kinetics; D008856:Microscopy, Fluorescence; D010010:Osteoclasts / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D011817:Rabbits; D013997:Time Factors; D014812:Vitamin K / Q000494:pharmacology*; D010837:Vitamin K 1 / Q000494:pharmacology
chemical_list: D014812:Vitamin K; D010837:Vitamin K 1