Acquired defects of glycogen synthase activity in cultured human skeletal muscle cells: influence of high glucose and insulin levels.
To determine whether defects of muscle glycogen synthase (GS) activity can be acquired by exposure to elevated glucose or insulin levels, human skeletal muscle cells obtained by needle biopsy from normal control subjects were grown in culture for 4-6 weeks followed by 4 days of fusion and differentiation in media containing either normal (5.5 mmol/l glucose and 22 pmol/l insulin) or increased concentrations of glucose (20 mmol/l), insulin (30 micromol/l), or both. After fusion in normal media, acute stimulation by 33 nmol/l insulin for 1 h increased GS fractional velocity (FV) approximately twofold (from 9.01 +/- 1.26 to 16.31 +/- 2.40, P < 0.05). Increasing the media glucose concentration alone to 20 mmol/l during fusion had no effect on basal FV but caused a marginal impairment of the insulin-stimulated GS response (from 8.51 +/- 1.33 to 12.99 +/- 1.90, P = 0.08). Increasing the media insulin concentration to 30 micromol/l during fusion at 5.5 mmol/l glucose also did not alter basal GS FV (10.61 +/- 1.69%) but completely abolished the normal insulin-stimulated increase in GS activity (to 11.63 +/- 1.55%, NS). The combination of high insulin (30 micromol/l) and high glucose (20 mmol/l) during fusion had no greater effect on the FV of either basal (11.66 +/- 2.16%, NS) or insulin-stimulated (9.20 +/- 1.80%, NS) GS activity than high insulin alone. Fusion in hyperinsulinemic media altered the kinetic parameters of GS with a near doubling of the basal Km0.1 and Vmax0.1 for uridinediphospho-glucose. Hyperinsulinemia also totally prevented the normal insulin-stimulated threefold increase in the Vmax0.1 and the 65% decrease in the A0.5 for glucose-6-phosphate. GS mRNA and protein expression, determined by RNase protection assay and immunoblotting, respectively, were unaffected by changes in media conditions. We conclude that exposure of human skeletal muscle cells primarily to high insulin induces severe insulin resistance through multiple acquired posttranslational defects, which affect both the kinetic characteristics and absolute activity of the GS enzyme.
Henry
R R
RR
Department of Medicine, University of California, San Diego, La Jolla, USA.
Ciaraldi
T P
TP
Mudaliar
S
S
Abrams
L
L
Nikoulina
S E
SE
D000199:Actins / Q000378:metabolism; D000328:Adult; D002454:Cell Differentiation; D002459:Cell Fusion; D002478:Cells, Cultured; D003402:Creatine Kinase / Q000096:biosynthesis / Q000378:metabolism; D046508:Culture Techniques / Q000379:methods; D015870:Gene Expression; D005947:Glucose / Q000494:pharmacology*; D006003:Glycogen / Q000378:metabolism*; D006006:Glycogen Synthase / Q000096:biosynthesis / Q000378:metabolism*; D006801:Humans; D007328:Insulin / Q000494:pharmacology*; D007527:Isoenzymes; D007700:Kinetics; D016018:Least-Squares Analysis; D008297:Male; D018482:Muscle, Skeletal / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D015347:RNA Probes; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D011972:Receptor, Insulin / Q000378:metabolism*; D012016:Reference Values; D012518:Sarcomeres / Q000378:metabolism; D014532:Uridine Diphosphate Glucose / Q000378:metabolism
D000199:Actins; D007328:Insulin; D007527:Isoenzymes; D015347:RNA Probes; D012333:RNA, Messenger; D006003:Glycogen; D006006:Glycogen Synthase; D011972:Receptor, Insulin; D003402:Creatine Kinase; D005947:Glucose; D014532:Uridine Diphosphate Glucose