Unusual antimicrobial compounds from Aeollanthus buchnerianus.
Using bioassay guided isolation, three novel 12 carbon polyoxygenated fatty acids and a novel abietane diterpene have been isolated from the chloroform extract of aerial parts of Aeollanthus buchnerianus (Lamiaceae). Rigorous spectroscopic methods were used for compound identification. (Z,Z)-8zeta-acetoxy-5zeta-hydroxydodeca-2,6-dienoic acid and (Z,Z)-5zeta, 8zeta-dihydroxydodeca-2,6-dienoic acid inhibited the spore germination of Cladosporium cucumerinum (both with Minimum Inhibitory Dose (MID) values of 1 microgram) and Aspergillus niger (MID 5 and 25 microgram respectively). Further, they also reduced the hyphal growth of Pythium ultimum. (Z)-5zeta-hydroxy-6zeta,7zeta,8zeta-triacetox ydodeca-2-dienoic acid exhibited short term inhibition of the growth of Cladosporium cucumerinum. The novel abietane diterpenoid, (rel)-14alpha-acetoxyabiet-7-en-18-oic acid inhibited the growth of the gram positive bacteria Bacillus subtilis, Staphylococcus aureus and Streptomyces scabies (MIC values 80, 20 and 20 micrograms ml(-1) respectively).
Dellar
J E
JE
Phytochemistry Research Laboratories, Department of Pharmaceutical Sciences, University of Strathclyde, Glasgow, Scotland.
Cole
M D
MD
Waterman
P G
PG
D000353:Africa, Southern; D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000935:Antifungal Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001234:Aspergillus niger / Q000187:drug effects / Q000254:growth & development; D002963:Cladosporium / Q000187:drug effects / Q000254:growth & development; D005231:Fatty Acids, Unsaturated / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D006094:Gram-Positive Bacteria / Q000187:drug effects*; D009682:Magnetic Resonance Spectroscopy; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D010936:Plant Extracts; D016339:Spectrometry, Mass, Fast Atom Bombardment; D013172:Spores, Fungal
D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D000935:Antifungal Agents; D005231:Fatty Acids, Unsaturated; D010936:Plant Extracts