title: Is aging related to cell signalling alterations in amphibian oocytes?
abstract: The storage of Bufo arenarum oocytes decreased their ability to become fertilized "in vitro". The stimulation with carbachol of "young oocytes" showed a persistent hydrolysis with phosphatidylinositol 4,5 diphosphate (PIP2) while in "aged oocytes" both phosphatidylinositol 4 phosphate (PIP) and PIP2 were hydrolyzed at a non-significant level. These results and the lower 32P-phosphoinositide levels found in "aged oocytes" at time of stimulation agree with diminished phosphoinositide kinase and phospholipase C activities, as the consequence of a non-specific phosphoinositide hydrolysis probably occurring during storage.
authors.affiliation: Libiquima, Facultad de Ingeniería, Universidad Nacional del Comahue, Buenos Aires, Neuquén, Argentina.
authors.forename: T M
authors.initials: TM
authors.lastname: Fonovich de Schroeder
authors.forename: A M
authors.initials: AM
authors.lastname: de D'Angelo
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D002022:Bufo arenarum / Q000502:physiology*; D002217:Carbachol / Q000494:pharmacology; D002450:Cell Communication / Q000187:drug effects / Q000502:physiology*; D016922:Cellular Senescence / Q000502:physiology; D005306:Fertilization / Q000187:drug effects / Q000502:physiology*; D018721:Muscarinic Agonists / Q000494:pharmacology; D009865:Oocytes / Q000187:drug effects / Q000502:physiology*; D010716:Phosphatidylinositols; D013997:Time Factors
chemical_list: D018721:Muscarinic Agonists; D010716:Phosphatidylinositols; D002217:Carbachol