title: Regional differences in constitutive and induced ICAM-1 expression in vivo.
abstract: The aim of the present study was to characterize and compare the expression of intercellular adhesion molecule 1 (ICAM-1) on unstimulated and endotoxin-challenged endothelial cells in different tissues of the rat. ICAM-1 expression was measured using 125I-labeled anti-rat ICAM-1 monoclonal antibody (MAb) and an isotype-matched control MAb labeled with 131I (to correct for nonspecific accumulation of the binding MAb). Under baseline conditions, ICAM-1 MAb binding was observed in all organs. The binding of 125I-ICAM-1 MAb varied widely among organs, with the largest accumulation (per g tissue) in the lung, followed by heart (1/30th of lung activity), splanchnic organs (1/50th of lung activity), thymus (1/100th of lung activity), testes (1/300th of lung activity), and skeletal muscle (1/800th of lung activity). Endotoxin induced an increase in ICAM-1 MAb binding in all organs except the spleen. Endotoxin-induced upregulation of ICAM-1 was greatest in heart and skeletal muscle (5- to 10-fold), whereas the remaining organs exhibited a two- to fourfold increase in ICAM-1 expression. Maximal upregulation of ICAM-1 occurred at 9-12 h after endotoxin administration. A dose-dependent increase in ICAM-1 expression was elicited by 0.1-10 microgram/kg, with higher doses (up to 5 mg/kg) producing no further increment. Induction of ICAM-1 mRNA after endotoxin was observed in all tissues examined (lung, heart, intestine), peaked at 3 h, and then rapidly returned to control levels. These findings indicate that ICAM-1 is constitutively expressed on vascular endothelium in all organs of the rat and that there are significant regional differences in the magnitude and time course of endotoxin-induced ICAM-1 expression.
authors.affiliation: Department of Physiology, Louisiana State University Medical Center, Shreveport 71130, USA.
authors.forename: J
authors.initials: J
authors.lastname: Panés
authors.forename: M A
authors.initials: MA
authors.lastname: Perry
authors.forename: D C
authors.initials: DC
authors.lastname: Anderson
authors.forename: A
authors.initials: A
authors.lastname: Manning
authors.forename: B
authors.initials: B
authors.lastname: Leone
authors.forename: G
authors.initials: G
authors.lastname: Cepinskas
authors.forename: C L
authors.initials: CL
authors.lastname: Rosenbloom
authors.forename: M
authors.initials: M
authors.lastname: Miyasaka
authors.forename: P R
authors.initials: PR
authors.lastname: Kvietys
authors.forename: D N
authors.initials: DN
authors.lastname: Granger
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism; D004731:Endotoxins / Q000494:pharmacology; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics / Q000378:metabolism*; D007457:Iodine Radioisotopes; D008297:Male; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D014018:Tissue Distribution
chemical_list: D000911:Antibodies, Monoclonal; D004731:Endotoxins; D007457:Iodine Radioisotopes; D012333:RNA, Messenger; D018799:Intercellular Adhesion Molecule-1