Anti-D quantification by flow cytometry: an alternative to the AutoAnalyser?
A flow cytometry method for quantifying levels of anti-D is described. The method is based on the indirect antiglobulin test and uses an FITC Fab anti-human IgG reagent for detection of bound anti-D. A standard curve is generated from the fluorescence measured by flow cytometry using the British Standard anti-D preparation 73/515. The fluorescence obtained from test samples by flow cytometry is converted into IU/mL anti-D, using the standard curve. In a limited study with 42 serum samples the assay showed good correlation with results obtained using the AutoAnalyser for sera with anti-D levels less than 100 IU/mL. The method is simple to perform and has potential as a replacement for the AutoAnalyser.
Austin
E B
EB
Manchester Blood Centre, Plymouth Grove, UK.
McIntosh
Y
Y
Hodson
C
C
Lee
D
D
D001322:Autoanalysis; D004906:Erythrocyte Count; D005260:Female; D005434:Flow Cytometry / Q000379:methods* / Q000706:statistics & numerical data; D006801:Humans; D007202:Indicators and Reagents; D007518:Isoantibodies / Q000097:blood*; D011247:Pregnancy; D012203:Rh Isoimmunization / Q000276:immunology; D012204:Rh-Hr Blood-Group System / Q000276:immunology*; D013696:Temperature
D007202:Indicators and Reagents; D007518:Isoantibodies; D012204:Rh-Hr Blood-Group System