Determination of beta-(1-3),(1-4)-D-glucans in barley by reversed-phase high-performance liquid chromatography.
An HPLC method for the determination of beta-glucan in barley was developed. The beta-glucan was hydrolysed with lichenase [endo-beta-(1-3),(1-4)-D-glucan-4-glucanhydrolase from Bacillus subtilis] to oligosaccharides, which were analysed by reversed-phase HPLC using water as the mobile phase at a flow-rate of 0.7 ml/min. The separation of the oligosaccharides was performed in a C18 stainless-steel column (Spherisorb ODS-2) with 5-microns particles in less than 10 min, with a refractive index detection.
Pérez-Vendrell
A M
AM
Institut de Recerca i Tecnologia Agroalimentàries (IRTA), Department of Animal Nutrition, Centre de Mas Bové, Reus, Spain.
Guasch
J
J
Francesch
M
M
Molina-Cano
J L
JL
Brufau
J
J
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D005936:Glucans / Q000032:analysis*; D006026:Glycoside Hydrolases / Q000378:metabolism; D001467:Hordeum / Q000737:chemistry*; D006868:Hydrolysis; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000032:analysis / Q000378:metabolism; D012031:Refractometry; D015203:Reproducibility of Results; D047071:beta-Glucans
D005936:Glucans; D009844:Oligosaccharides; D047071:beta-Glucans; C067858:beta-glucan, (1-3)(1-4)-; D006026:Glycoside Hydrolases; C028020:licheninase