[Development of a sandwich ELISA for detection of specific antibodies against fimbrial antigens K88, K99 and 987p of Escherichia coli].
For the assessment of the efficacy of a E. coli-vaccine it was important to detect the specific antibodies against the protective antigens. Under the use of monoclonal antibodies against the fimbrial antigens K88, K99 and 987p a sandwich-elisa was developed. With these elisa-tests we detected significant titre conversion after repeated immunisation of rabbits. The results are highly reproducible. That is why the elisa-tests are qualified for testing of vaccine batches. The use of an anti-pig-conjugate makes it possible to detect antibodies in colostrum and serum from pigs.
Jäkel
C
C
Bereich Forschung und Entwicklung des Impfstoffwerkes Dessau-Tornau GmbH.
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis* / Q000097:blood; D000911:Antibodies, Monoclonal; D001428:Bacterial Vaccines; D003126:Colostrum / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D004926:Escherichia coli / Q000276:immunology*; D004927:Escherichia coli Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D010861:Fimbriae, Bacterial / Q000276:immunology*; D011817:Rabbits / Q000276:immunology; D015203:Reproducibility of Results; D013552:Swine; D013553:Swine Diseases
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D001428:Bacterial Vaccines