title: Changes in the expression of fibroblast growth factor receptors mark distinct stages of chondrogenesis in vitro and during chick limb skeletal patterning.
abstract: Members of the fibroblast growth factor (FGF) family of growth factors are key regulators of limb skeletal patterning and growth. Abnormal expression of FGFs or mutations in their receptors (fgfrs) result in skeletal disorders. Here we show that changes in the expression of fgfrs are intrinsic properties of differentiating cartilage. In mesenchymal micromass cultures differentiating into cartilage, as in ovo, fgfr 1 mRNA was found predominantly in undifferentiated, proliferating mesenchyme, fgfr 2 in precartilage cell aggregates, and fgfr 3 in differentiating cartilage nodules. Thus, our data suggest that switches in the expression of fgfr 1, 2, and 3 mRNAs are associated with phases of cartilage patterning both in vitro and in ovo, and mark distinct stages in the development of the limb skeleton.
authors.affiliation: Department of Anatomy, University of Wisconsin Medical School, Madison 53706, USA.
authors.forename: G
authors.initials: G
authors.lastname: Szebenyi
authors.forename: M P
authors.initials: MP
authors.lastname: Savage
authors.forename: B B
authors.initials: BB
authors.lastname: Olwin
authors.forename: J F
authors.initials: JF
authors.lastname: Fallon
mesh_terms: D017398:Alternative Splicing / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D002356:Cartilage / Q000166:cytology / Q000196:embryology* / Q000648:ultrastructure; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured / Q000502:physiology; D002642:Chick Embryo; D018076:DNA, Complementary / Q000502:physiology; D005121:Extremities / Q000196:embryology; D005346:Fibroblast Growth Factors / Q000502:physiology*; D018507:Gene Expression Regulation, Developmental / Q000502:physiology; D017403:In Situ Hybridization; D018878:Limb Buds / Q000502:physiology; D008648:Mesoderm / Q000166:cytology / Q000502:physiology; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis; D017468:Receptors, Fibroblast Growth Factor / Q000235:genetics*; D013997:Time Factors; D014921:Wings, Animal / Q000196:embryology
chemical_list: D018076:DNA, Complementary; D012333:RNA, Messenger; D017468:Receptors, Fibroblast Growth Factor; D005346:Fibroblast Growth Factors