jun-B gene expression in rat retinal cells following focal retinal injury.
We examined jun-B gene expression in the rat retina after a focal retinal injury by in situ hybridization. 30 min after producing a penetrating retinal wound, the retina showed the expression of jun-B mRNA intensively in the inner nuclear layer (INL) and relatively sparsely in the ganglion cell layer. The double-labeled retina by immunocytochemistry and in situ hybridization demonstrated that jun-B mRNA within the INL surrounded S-100 protein immunoreactive cytoplasm in the middle sublayer of the INL. These results suggest that the jun-B expression is induced mainly in Müller cells following retinal damage.
Ohki
K
K
Department of Ophthalmology, Hokkaido University School of Medicine, Sapporo, Japan.
Yoshida
K
K
Yamakawa
A
A
Harada
T
T
Matsuda
H
H
Imaki
J
J
D000818:Animals; D003593:Cytoplasm / Q000378:metabolism; D015807:Eye Injuries, Penetrating / Q000378:metabolism*; D005786:Gene Expression Regulation; D016758:Genes, jun / Q000235:genetics; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D016755:Proto-Oncogene Proteins c-jun / Q000096:biosynthesis* / Q000235:genetics*; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D012160:Retina / Q000166:cytology / Q000293:injuries* / Q000378:metabolism*; D009418:S100 Proteins / Q000378:metabolism
D016755:Proto-Oncogene Proteins c-jun; D012333:RNA, Messenger; D009418:S100 Proteins