title: Response of human artery, vein, and cultured smooth muscle cells to atrial and C-type natriuretic peptides.
abstract: OBJECTIVE
  We determined the response of intracellular cyclic GMP in human arteries and veins and in smooth muscle cells cultured from these vessels to C-type natriuretic peptide in comparison with atrial natriuretic peptide.
  
  
  METHODS
  Repeated-measures analysis of concentration-response curves.
  
  
  METHODS
  Anesthesia research laboratory.
  
  
  METHODS
  Vascular smooth muscle cells from human blood vessels obtained with Institutional Review Board approval and patient consent.
  
  
  RESULTS
  Segments of internal mammary artery and saphenous vein were obtained from patients undergoing coronary artery bypass surgery. Smooth muscle cells were cultured from these vessels. Concentration-response curves of intracellular cyclic GMP were determined and analyzed by two-way analysis of variance with repeated measures. In segments of intact saphenous vein, C-type natriuretic peptide was significantly more effective than atrial natriuretic peptide (16-fold increase in cyclic GMP in response to 1 microM of C-type natriuretic peptide vs. six-fold increase in cyclic GMP in response to 1 microM of atrial natriuretic peptide, p < .05). In rings of intact internal mammary artery, 1 microM of atrial natriuretic peptide (26-fold increase in cyclic GMP over basal value) was more effective than 1 microM of c-type natriuretic peptide (three-fold increase in cyclic GMP over basal value, p < .05). In cultured cells from these vessels, the pattern of response to C-type natriuretic peptide and atrial natriuretic peptide was the same as in the intact vessels.
  
  
  CONCLUSIONS
  These results indicated that human smooth muscle cells in arteries and veins express both forms of natriuretic peptide receptors but that atrial natriuretic peptide acts primarily on the artery and C-type natriuretic peptide acts predominantly on the vein. Increased concentrations of C-type natriuretic peptide could contribute to venous pooling in septic shock.
authors.affiliation: Department of Anesthesiology, Mercer University School of Medicine, Macon, GA., USA.
authors.forename: L M
authors.initials: LM
authors.lastname: Zhang
authors.forename: M R
authors.initials: MR
authors.lastname: Castresana
authors.forename: M H
authors.initials: MH
authors.lastname: McDonald
authors.forename: J H
authors.initials: JH
authors.lastname: Johnson
authors.forename: W H
authors.initials: WH
authors.lastname: Newman
mesh_terms: D000704:Analysis of Variance; D009320:Atrial Natriuretic Factor / Q000502:physiology*; D002478:Cells, Cultured / Q000187:drug effects; D006152:Cyclic GMP / Q000502:physiology*; D006801:Humans; D066298:In Vitro Techniques; D008323:Mammary Arteries / Q000187:drug effects*; D009131:Muscle, Smooth, Vascular / Q000187:drug effects*; D020098:Natriuretic Peptide, C-Type; D011506:Proteins / Q000502:physiology*; D012501:Saphenous Vein / Q000187:drug effects*
chemical_list: D011506:Proteins; D020098:Natriuretic Peptide, C-Type; D009320:Atrial Natriuretic Factor; D006152:Cyclic GMP