title: On-column isomerization of sugars during high-performance liquid chromatography: analysis of the elution profile.
abstract: Four monosaccharides (glucose, galactose, mannose and fructose) and one disaccharide (maltose) were subjected to high-performance liquid chromatography with UV or refractive index detection. Various profiles such as broad, tailed and splitted peaks were produced, depending on column temperature and eluent flow-rate because these saccharides underwent isomerization. In contrast, alpha-methylglucoside, a non-converting derivative, always produced a sharp peak. By analyzing these profiles kinetic constants of the isomerization were obtained and compared with the literature data.
authors.affiliation: Department of Clinical Pathology, School of Medicine, Kitasato University, Kanagawa, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Nishikawa
authors.forename: S
authors.initials: S
authors.lastname: Suzuki
authors.forename: H
authors.initials: H
authors.lastname: Kubo
authors.forename: H
authors.initials: H
authors.lastname: Ohtani
mesh_terms: D002241:Carbohydrates / Q000032:analysis* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D007536:Isomerism; D007700:Kinetics; D008320:Maltose / Q000032:analysis / Q000737:chemistry; D009005:Monosaccharides / Q000032:analysis / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet
chemical_list: D002241:Carbohydrates; D009005:Monosaccharides; D008320:Maltose