Anti-epithelial (anti-A549) antibodies: their nature, specificity and relevance to transplantation.
Several studies have addressed the possible importance of anti-epithelial cell antibodies in kidney transplantation using the A549 cell line as an in vitro model. In this paper we report our results using for the first time an enzyme-linked immunosorbent assay (ELISA) to detect the anti-A549 cell antibodies. Sera from 129 kidney transplant patients were tested for IgM anti-epithelial cell antibodies directed against the A549 cell line prior to transplantation; only three sera were positive (2.3%). 101 of these patients were then followed-up post-transplantation; sera were collected routinely at 2, 6 and 12 weeks and at the time of rejection episodes. All samples were also tested for cytomegalovirus (CMV) IgM antibodies. Sixteen patients developed anti-A549 IgM antibodies, and there was no correlation with acute graft rejection. Anti-epithelial antibodies showed no binding to sections of normal kidney or biopsies of rejected kidneys. Eleven patients were positive for anti-CMV IgM antibodies. In nine cases both IgM anti-A549 and IgM anti-CMV antibodies were found, which was a highly significant association (p < 0.001). Analysis of A549 cellular proteins by immunoblotting gave evidence for the presence of CMV polypeptides in the cell lysate. Electron-microscopic examination of A549 cell preparations revealed intracellular particles which were compatible in size with CMV. Polymerase chain reaction analysis confirmed the presence of a specific CMV DNA sequence in A549 cells of several batches from different sources. Our data strongly suggest that the A549 cell line used in several published reports is infected with CMV and that in the majority of cases the anti-A549 'anti-epithelial' antibodies found in renal transplant patients are anti-CMV antibodies.
Abdul-Karim
A B
AB
Renal Unit, Queen Elizabeth Hospital, Edgbaston, Birmingham, UK.
Barnes
A D
AD
Bevan
I S
IS
Howie
A J
AJ
Adu
D
D
Ross
H F
HF
Raykundalia
C
C
Catty
D
D
D000293:Adolescent; D000328:Adult; D000914:Antibodies, Viral / Q000097:blood; D000918:Antibody Specificity; D001172:Arthritis, Rheumatoid / Q000276:immunology; D001483:Base Sequence; D002648:Child; D003587:Cytomegalovirus / Q000235:genetics / Q000302:isolation & purification / Q000648:ultrastructure; D004848:Epithelium / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood / Q000494:pharmacology; D007075:Immunoglobulin M / Q000097:blood; D016030:Kidney Transplantation / Q000276:immunology*; D008175:Lung Neoplasms / Q000276:immunology; D008180:Lupus Erythematosus, Systemic / Q000276:immunology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011446:Prospective Studies; D012217:Rheumatoid Factor / Q000097:blood; D014407:Tumor Cells, Cultured; D014764:Viral Proteins / Q000032:analysis
D000914:Antibodies, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D014764:Viral Proteins; D012217:Rheumatoid Factor