title: The human immediate early gene BRF1 maps to chromosome 14q22-q24.
abstract: BRF1 (Butyrate response factor 1) is a member of an immediate early gene family specifying putative nuclear transcription factors. A repeat motif incorporating two Cys and two His is highly conserved between family members identified from yeast, Drosophila, mouse, rat, and human. The chromosome localization of none of the human genes has been determined thus far. Using the polymerase chain reaction on a human-rodent hybrid panel, we have localized BRF1 to chromosome 14. This was confirmed by direct sequencing of the PCR fragment. Using fluorescence in situ hybridization, the chromosome localization of BRF1 was further determined as 14q22-q24.
authors.affiliation: Academic Surgical Unit, London Hospital Medical College, University of London, United Kingdom.
authors.forename: K N
authors.initials: KN
authors.lastname: Maclean
authors.forename: C G
authors.initials: CG
authors.lastname: See
authors.forename: I A
authors.initials: IA
authors.lastname: McKay
authors.forename: S A
authors.initials: SA
authors.lastname: Bustin
mesh_terms: D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D002883:Chromosomes, Human, Pair 14; D006801:Humans; D006822:Hybrid Cells; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D029701:Saccharomyces cerevisiae Proteins; D035381:TATA-Binding Protein Associated Factors; D035361:Transcription Factor TFIIIB; D014157:Transcription Factors / Q000235:genetics*
chemical_list: C090956:BRF1 protein, S cerevisiae; C441028:BRF1 protein, human; D029701:Saccharomyces cerevisiae Proteins; D035381:TATA-Binding Protein Associated Factors; D035361:Transcription Factor TFIIIB; D014157:Transcription Factors