Functional and structural roles of the highly conserved Trp120 loop region of glucoamylase from Aspergillus awamori.
The functional role of a loop region, highly conserved among glucoamylase and other starch hydrolases which also includes the essential Trp120 of Aspergillus awamori, is investigated. Residues 121-125 of A. awamori glucoamylase were singly substituted, and their individual effects on catalytic activity and thermal stability were determined. The Arg122-->Tyr mutation displayed opposing effects for shorter and longer maltooligosaccharide substrates, K(m) decreasing for shorter substrates but increasing for longer substrates. The Pro123-->Gly mutation decreases the thermal stability of glucoamylase by 19 degrees C with little effect on activity. The Gln124-->His substitution decreases k(cat) for all substrates 10-15-fold. Gly121-->Thr and Arg125-->Lys had only minor effects on glucoamylase activity. While Arg122-->Tyr, Gln124-->His, and the previously constructed Trp120-->Phe [Sierks, M. R., Svensson, B., Ford, C., & Reilly, P. J. (1989) Protein Eng. 2, 621-625] glucoamylases have significantly reduced activity toward maltose hydrolysis, all mutations in the Trp120 loop region retain wild-type level activity toward alpha-D-glucosyl fluoride hydrolysis. The Trp120 loop region therefore plays a major role in directing conformational changes controlling the postulated rate-limiting product release step, even though only Trp120 is indicated to interact with acarbose in the crystal structure [Aleshin, A. E., Firsov, L. M., & Honzatko, R. B. (1994) J. Biol. Chem. 269, 15631-15639]. Side chains of residues 116, 120, 122, and 124 oriented in one direction play crucial roles in the enzyme mechanism, while side chains of residues 119, 121, 123, and 125, oriented in the opposite direction, play only minor roles.
Natarajan
S
S
Department of Chemical and Biochemical Engineering, University of Maryland Baltimore County, 21228, USA.
Sierks
M R
MR
D000595:Amino Acid Sequence; D001230:Aspergillus / Q000201:enzymology*; D001483:Base Sequence; D001665:Binding Sites; D017124:Conserved Sequence; D005087:Glucan 1,4-alpha-Glucosidase / Q000096:biosynthesis / Q000737:chemistry* / Q000378:metabolism*; D006026:Glycoside Hydrolases / Q000737:chemistry; D007700:Kinetics; D008958:Models, Molecular; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009838:Oligodeoxyribonucleotides; D017354:Point Mutation; D017433:Protein Structure, Secondary; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D013379:Substrate Specificity; D013816:Thermodynamics; D014364:Tryptophan
D009838:Oligodeoxyribonucleotides; D011994:Recombinant Proteins; D014364:Tryptophan; D006026:Glycoside Hydrolases; D005087:Glucan 1,4-alpha-Glucosidase