A new approach to analysis of human sweating.
In human skin transplanted to the back of 3 strains of immuno-deficient mice the function of the eccrine sweat glands of the human transplant was tested by topical intradermal application of pilocarpine, adrenaline and atropine + pilocarpine. Sweat responses were observed in pre-selected fields of observation by means of video macroscope. The iodine starch reaction served as an indicator for the appearance of sweat sport and permitted the evaluation of areas wetted by sweat in the field of observation. Among 9 animals tested, the hybrids between the CB-17-scid mouse and the BALB/cA-nu mouse (BALB/cA-nu,scid) seemed to exhibit the most consistent sweating response to local pharmacological stimulation. According to histological examination, eccrine sweat glands were preserved in human skin transplanted into the back skin of the BALB/cA-nu,scid mouse strain. The heterologous, human skin graft provides a novel model permitting, independent of the normal sweat gland innervation, the analysis of molecular receptors of sweat gland cells by which the actions of natural transmitters and pharmacological agents are transduced.
Shimazu
M
M
Department of Environmental Physiology, Institute of Tropical Medicine, Nagasaki University, Japan.
Matsumoto
T
T
Kosaka
M
M
Ohwatari
N
N
Tsuchiya
K
K
Ueyama
Y
Y
Urano
K
K
Kataki
Y
Y
Saito
M
M
D000818:Animals; D001285:Atropine / Q000494:pharmacology; D004439:Eccrine Glands / Q000187:drug effects / Q000378:metabolism* / Q000637:transplantation; D004837:Epinephrine / Q000494:pharmacology; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D016513:Mice, SCID; D010862:Pilocarpine / Q000494:pharmacology; D016038:Skin Transplantation / Q000502:physiology*; D013045:Species Specificity; D013542:Sweat / Q000187:drug effects / Q000378:metabolism*; D014183:Transplantation, Heterologous / Q000502:physiology*; D014741:Video Recording
D010862:Pilocarpine; D001285:Atropine; D004837:Epinephrine