Iron deprivation results in an increase in p53 expression.
Deferoxamine (DFO)-induced iron deprivation caused an increase in p53 expression in ML-1 and Raji cells. In ML-1 cells, with express wild type p53, p53 protein levels were transiently increased 6 h after addition of 10(-4)M DFO. In Raji cells, which carry a mutant p53 allele, p53 increased 6 h after addition of 10(-4)M DFO and remained elevated for 24 h. Growth inhibition was observed in both cell types 6 h after addition of 10(-4)M DFO. In both cells, p53 mRNA levels did not increase following incubation with DFO, suggesting that increased p53 expression is the result of a post-transcriptional mechanism. Although increases in wild type p53 protein in ML-1 cells resulted in increases in a p53 target gene, p21cipl/wafl/sdil, this effect was not observed in Raji cells which express a mutant p53 protein.
Fukuchi
K
K
Department of Clinical Pathology, Showa University, Tokyo, Japan.
Tomoyasu
S
S
Watanabe
H
H
Kaetsu
S
S
Tsuruoka
N
N
Gomi
K
K
D017209:Apoptosis / Q000502:physiology; D004247:DNA / Q000096:biosynthesis; D003676:Deferoxamine / Q000494:pharmacology; D005434:Flow Cytometry; D015870:Gene Expression / Q000187:drug effects / Q000502:physiology*; D016158:Genes, p53; D006801:Humans; D000090463:Iron Deficiencies; D009154:Mutation; D011499:Protein Processing, Post-Translational; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
D004247:DNA; D003676:Deferoxamine