Anisakis simplex: stage-specific antigens recognized by mice.
To investigate antigenic differences between the developmental stages of Anisakis simplex, somatic, excretion-secretion and detergent-solubilized surface antigen preparations of third- and fourth-stage A. simplex larvae, and a somatic antigen preparation of adults, were characterized by SDS-PAGE and immunoblotting using mouse immune serum containing antibodies against the two larval stages. Excretion-secretion and surface antigen preparations from third-stage larvae behaved very differently from those of fourth-stage larvae, in both SDS-PAGE and immunoblotting; this suggests that certain excretion-secretion and surface antigens are highly stage-specific. By contrast, somatic components of third- and fourth-stage larvae were found to have very similar banding patterns, suggesting conservation of these components during the development of the parasite in mice. The SDS-PAGE and immunoblotting results for adult somatic components seem to support this hypothesis.
Iglesias
R
R
Instituto de Investigación y Análisis Alimentario, Facultad de Farmacia, Universidad de Santiago de Compostela, Spain.
Leiro
J
J
Ubeira
F M
FM
Santamarina
M T
MT
Sanmartín
M L
ML
D000818:Animals; D017129:Anisakiasis / Q000276:immunology*; D017163:Anisakis / Q000254:growth & development*; D000909:Antibodies, Helminth / Q000276:immunology*; D000918:Antibody Specificity; D000947:Antigens, Helminth / Q000276:immunology* / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D015151:Immunoblotting; D007814:Larva / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008808:Mice, Inbred CBA
D000909:Antibodies, Helminth; D000947:Antigens, Helminth