Identification of a Renibacterium salmoninarum DNA fragment associated with bacterial internalization into CHSE-cultured cells.
We report here the isolation of a Renibacterium salmoninarum DNA sequence capable of transforming a non-invasive Escherichia coli strain into a microorganism able to enter the fish cell line, CHSE-214. Immunofluorescence and electron microscopy techniques were used to assess the acquired invasive phenotype by HB101 E. coli cells, upon transformation with pPMV-189. This plasmid carries a 2282-bp R. salmoninarum DNA segment. The invasive phenotype is conserved upon deletion of approximately 1000 bp at the 3' end of the insert. The remaining segment contains an ORF region encoding a putative protein of about 30 kDa.
Maulén
N P
NP
Instituto de Bioquímica, Facultad de Ciencias, Universidad Austral de Chile, Valdivia, Chile.
Morales
P J
PJ
Aruti
D
D
Figueroa
J E
JE
Concha
M I
MI
Krauskopf
M
M
León
G
G
D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D004269:DNA, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D006094:Gram-Positive Bacteria / Q000235:genetics / Q000472:pathogenicity*; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D008969:Molecular Sequence Data; D010957:Plasmids; D012487:Salmonidae; D017422:Sequence Analysis, DNA; D014169:Transformation, Bacterial
D004269:DNA, Bacterial