title: A genomic region of lactococcal temperate bacteriophage TP901-1 encoding major virion proteins.
abstract: Two major structural proteins, MHP (major head protein) and MTP (major tail protein), from the lactococcal temperate phage TP901-1 were sequenced at their amino acid termini, and derived degenerate oligonucleotides were used to locate the corresponding genes in the phage genome. This genomic region was sequenced. The sequence characterized includes a total of 11 open reading frames (ORFs) showing an operon structure. Upstream of each ORF, except ORF b2 and ORF x, potential ribosome-binding sites were found, suggesting independent translation. However, coupled translation is suggested for ORF x and as a possibility for ORF b3 and ORF c2, which have ribosome-binding sites located more distant from their start codons. ORF b2 may be translationally fused with mhp at a low frequency. The mhp and mtp genes are transcribed as a 3.7-kb mRNA with at least six additional ORFs. The organization of the genomic region analyzed resembles that of other distantly related phages, providing possible roles for the uncharacterized ORFs.
authors.affiliation: Danish Center for Lactic Acid Bacteria, Department of Dairy and Food Science, Royal Veterinary and Agricultural University, Frederiksberg, Denmark.
authors.forename: M G
authors.initials: MG
authors.lastname: Johnsen
authors.forename: K F
authors.initials: KF
authors.lastname: Appel
authors.forename: P L
authors.initials: PL
authors.lastname: Madsen
authors.forename: F K
authors.initials: FK
authors.lastname: Vogensen
authors.forename: K
authors.initials: K
authors.lastname: Hammer
authors.forename: J
authors.initials: J
authors.lastname: Arnau
mesh_terms: D000595:Amino Acid Sequence; D001435:Bacteriophages / Q000235:genetics*; D001482:Base Composition; D001483:Base Sequence; D001665:Binding Sites; D005814:Genes, Viral / Q000235:genetics*; D013294:Lactococcus lactis / Q000821:virology*; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D017433:Protein Structure, Secondary; D012333:RNA, Messenger / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis; D015183:Restriction Mapping; D012270:Ribosomes / Q000378:metabolism; D017421:Sequence Analysis; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D015678:Viral Structural Proteins / Q000032:analysis / Q000737:chemistry / Q000235:genetics*; D017376:Viral Tail Proteins / Q000032:analysis / Q000737:chemistry / Q000235:genetics*; D014771:Virion / Q000737:chemistry
chemical_list: D012333:RNA, Messenger; D012367:RNA, Viral; D015678:Viral Structural Proteins; D017376:Viral Tail Proteins; C099095:major head protein, lactococcal bacteriophage; C099096:major tail protein, lactococcal bacteriophage