The PRP31 gene encodes a novel protein required for pre-mRNA splicing in Saccharomyces cerevisiae.
The pre-mRNA splicing factor Prp31p was identified in a screen of temperature-sensitive yeast strains for those exhibiting a splicing defect upon shift to the non- permissive temperature. The wild-type PRP31 gene was cloned and shown to be essential for cell viability. The PRP31 gene is predicted to encode a 60 kDa polypeptide. No similarities with other known splicing factors or motifs indicative of protein-protein or RNA-protein interaction domains are discernible in the predicted amino acid sequence. A PRP31 allele bearing a triple repeat of the hemagglutinin epitope has been generated. The tagged protein is functional in vivo and a single polypeptide species of the predicted size was detected by Western analysis with proteins from yeast cell extracts. Functional Prp31p is required for the processing of pre-mRNA species both in vivo and in vitro, indicating that the protein is directly involved in the splicing pathway.
Weidenhammer
E M
EM
Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, PA 15213 USA.
Singh
M
M
Ruiz-Noriega
M
M
Woolford
J L
JL
D003001:Cloning, Molecular; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism*; D005800:Genes, Fungal; D009154:Mutation; D012322:RNA Precursors / Q000378:metabolism*; D012323:RNA Processing, Post-Transcriptional; D012326:RNA Splicing / Q000235:genetics; D012331:RNA, Fungal / Q000378:metabolism*; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism*; D013696:Temperature
D005656:Fungal Proteins; D012322:RNA Precursors; D012331:RNA, Fungal