[Detection and typing of HPV DNA in situ--improvement in diagnosis of lesions of the uterine cervix].
We examined the cervical samples from 63 female patients with various grades of cervical intraepithelial neoplasia (CIN), invasive squamous cell carcinoma (INCA) or inflammation. All the women with the diagnosis of CIN were selected on the basis of cytological prediction of HPV infection (koilocytosis, dyskeratosis). The analysis of human papillomavirus (HPV) genome by DNA hybridization in situ was done in all cases. Simultaneously, the immunohistochemical expression of papillomavirus common antigen (PVCA) and proliferating cell nuclear antigen PCNA/cykline was determined. The results confirm the correlation between high risk HPV types 16, 18, 33 and higher grade of dysplasia (CIN) as well as higher proliferative activity. On the other hand, the detection of HPV DNA does not correlate with PVCA expression or with cytological/histological diagnosis of koilocytosis. This finding shows that common cytological or histological examinations may be unreliable and in particular the significance of koilocytosis must be reevaluated.
Kotrsová
L
L
Gynek.-porod. klinika LF UP, Olomouc.
Kolár
Z
Z
Tichý
M
M
Tichá
V
V
Krcmár
M
M
Hamsíková
E
E
Vonka
V
V
D000956:Antigens, Viral / Q000032:analysis; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D006801:Humans; D017403:In Situ Hybridization; D027383:Papillomaviridae / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000175:diagnosis; D014412:Tumor Virus Infections / Q000175:diagnosis; D002577:Uterine Cervical Diseases / Q000175:diagnosis*; D002583:Uterine Cervical Neoplasms / Q000175:diagnosis; D002578:Uterine Cervical Dysplasia / Q000175:diagnosis
D000956:Antigens, Viral; D004279:DNA, Viral