title: Crystal structure of a mammalian phosphoinositide-specific phospholipase C delta.
abstract: Mammalian phosphoinositide-specific phospholipase C enzymes (PI-PLC) act as signal transducers that generate two second messengers, inositol-1,4,5-trisphosphate and diacylglycerol. The 2.4-A structure of phospholipase C delta 1 reveals a multidomain protein incorporating modules shared by many signalling proteins. The structure suggests a mechanism for membrane attachment and Ca2+-dependent hydrolysis of second-messenger precursors. The regulation and reversible membrane association of PI-PLC may serve as a model for understanding other multidomain enzymes involved in phospholipid signalling.
authors.affiliation: Center for Protein Engineering, MRC Centre, Cambridge, UK.
authors.forename: L O
authors.initials: LO
authors.lastname: Essen
authors.forename: O
authors.initials: O
authors.lastname: Perisic
authors.forename: R
authors.initials: R
authors.lastname: Cheung
authors.forename: M
authors.initials: M
authors.lastname: Katan
authors.forename: R L
authors.initials: RL
authors.lastname: Williams
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D002384:Catalysis; D002462:Cell Membrane / Q000378:metabolism; D018360:Crystallography, X-Ray; D006801:Humans; D006868:Hydrolysis; D015544:Inositol 1,4,5-Trisphosphate / Q000378:metabolism; D007527:Isoenzymes / Q000737:chemistry* / Q000378:metabolism; D008958:Models, Molecular; D008968:Molecular Conformation; D008969:Molecular Sequence Data; D054801:Phosphoinositide Phospholipase C; D054803:Phospholipase C delta; D010727:Phosphoric Diester Hydrolases / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding; D011487:Protein Conformation; D051381:Rats; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D015290:Second Messenger Systems; D017386:Sequence Homology, Amino Acid
chemical_list: D007527:Isoenzymes; D011994:Recombinant Proteins; D015544:Inositol 1,4,5-Trisphosphate; D010727:Phosphoric Diester Hydrolases; D054801:Phosphoinositide Phospholipase C; D054803:Phospholipase C delta