title: Inhibitor sensitivity of respiratory complex I in human platelets: a possible biomarker of ageing.
abstract: NADH-Coenzyme Q reductase was assayed in platelet mitochondrial membranes obtained from 19 pools of two venous blood samples from female young (19-30 years) individuals and 18 pools from aged ones (66-107 years). The enzyme activities were not significantly changed in the two groups, but a decrease of sensitivity to the specific inhibitor, rotenone, occurred in a substantial number of aged individuals. The results are in agreement with the predictions of the mitochondrial theory of ageing and may be used to develop a sensitive biomarker of the ageing process.
authors.affiliation: Department of Biochemistry 'G. Morruzzi', University of Bologna, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Merlo Pich
authors.forename: C
authors.initials: C
authors.lastname: Bovina
authors.forename: G
authors.initials: G
authors.lastname: Formiggini
authors.forename: G G
authors.initials: GG
authors.lastname: Cometti
authors.forename: A
authors.initials: A
authors.lastname: Ghelli
authors.forename: G
authors.initials: G
authors.lastname: Parenti Castelli
authors.forename: M L
authors.initials: ML
authors.lastname: Genova
authors.forename: M
authors.initials: M
authors.lastname: Marchetti
authors.forename: S
authors.initials: S
authors.lastname: Semeraro
authors.forename: G
authors.initials: G
authors.lastname: Lenaz
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000502:physiology*; D015415:Biomarkers; D001792:Blood Platelets / Q000502:physiology*; D042967:Electron Transport Complex I; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005260:Female; D006801:Humans; D008928:Mitochondria / Q000502:physiology; D009247:NADH, NADPH Oxidoreductases / Q000037:antagonists & inhibitors*; D012402:Rotenone / Q000494:pharmacology*
chemical_list: D015415:Biomarkers; D004791:Enzyme Inhibitors; D012402:Rotenone; D009247:NADH, NADPH Oxidoreductases; D042967:Electron Transport Complex I