An insecticidal N-acetylglucosamine-specific lectin gene from Griffonia simplicifolia (Leguminosae).
Griffonia simplicifolia II, an N-acetylglucosamine-specific legume lectin, has insecticidal activity when fed to the cowpea weevil, Callosobruchus maculatus (F.). A cDNA clone encoding G. simplicifolia II was isolated from a leaf cDNA library, sequenced, and expressed in a bacterial expression system. The recombinant protein exhibited N-acetylglucosamine-binding and insecticidal activity against cowpea weevil, indicating that glycosylation and multimeric structure are not required for these properties. These results support the hypothesis that genes of the legume lectin gene family encode proteins that function in plant defense against herbivores.
Zhu
K
K
Department of Entomology, Purdue University, West Lafayette, Indiana 47907, USA.
Huesing
J E
JE
Shade
R E
RE
Bressan
R A
RA
Hasegawa
P M
PM
Murdock
L L
LL
D000117:Acetylglucosamine; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D007887:Fabaceae / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D015723:Gene Library; D017343:Genes, Plant; D006031:Glycosylation; D007313:Insecta / Q000187:drug effects; D007306:Insecticides / Q000494:pharmacology*; D037102:Lectins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D005810:Multigene Family; D018515:Plant Leaves / Q000737:chemistry; D037121:Plant Lectins; D010946:Plants, Medicinal; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000096:biosynthesis; D012639:Seeds / Q000737:chemistry; D017421:Sequence Analysis; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D014018:Tissue Distribution
D018076:DNA, Complementary; C045475:Griffonia simplicifolia lectins; D007306:Insecticides; D037102:Lectins; D037121:Plant Lectins; D011994:Recombinant Proteins; D000117:Acetylglucosamine