Use of RAPD-PCR to differentiate genetically defined lines of an intermediate host of Schistosoma mansoni, Biomphalaria glabrata.
The genetic differentiation among several laboratory-maintained pedigree snail lines of Biomphalaria glabrata (with different susceptibility phenotypes to Schistosoma mansoni infection) was assessed with the random amplified polymorphic DNA method. Out of the 20 primers tested, 2 (OPA-01 and OPA-06) gave reproducible markers with either individual or bulked DNA samples from resistant (BS-90, 10-R2, LAC-line) and susceptible (M-line) snails. Arbitrary primer, OPA-01, amplification of BS-90 DNA identified a 180-bp strain-specific fragment and a 400-bp marker in the susceptible M-line stock. In the 10-R2 and LAC snail lines, OPA-01 specific markers of 200 bp and 550 bp were identified. Amplification with primer OPA-06 identified several major strain-specific markers in the BS-90 (150 bp, 400 bp, 800 bp) and M-line (1,100 bp) snails. The heritability of the RAPD markers was evaluated in progeny snails derived from a cross between the BS-90 and M-line stocks. Results showed that markers were inherited in a dominant or codominant fashion. The 1,100-bp M-line marker was inherited in all susceptible progeny snails analyzed.
Larson
S E
SE
Biomedical Research Institute, Rockville, Maryland 20852, USA.
Anderson
P L
PL
Miller
A N
AN
Cousin
C E
CE
Richards
C S
CS
Lewis
F A
FA
Knight
M
M
D000818:Animals; D001483:Base Sequence; D001700:Biomphalaria / Q000145:classification / Q000235:genetics* / Q000469:parasitology; D004247:DNA / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D017931:DNA Primers / Q000737:chemistry; D004198:Disease Susceptibility; D004199:Disease Vectors; D005819:Genetic Markers; D014644:Genetic Variation; D007113:Immunity, Innate; D008969:Molecular Sequence Data; D010641:Phenotype; D019105:Random Amplified Polymorphic DNA Technique; D015203:Reproducibility of Results; D012550:Schistosoma mansoni / Q000502:physiology*; D013045:Species Specificity
D017931:DNA Primers; D005819:Genetic Markers; D004247:DNA