title: Accelerated healing of cardiovascular textiles promoted by an RGD peptide.
abstract: Polytetrafluoroethylene (PTFE) and polyethylene terephthalate (Dacron polyester) fabrics are used extensively in cardiovascular devices, e.g. heart valve sewing cuffs and vascular prostheses. While devices containing these fabrics are generally successful, it is recognized that fabrics cause complications prior to tissue ingrowth due to their thrombogenic nature. A surface active synthetic peptide, called PepTite Coating (PepTite), which was modeled after the cell attachment domain of human fibronectin has been marketed as a biocompatible coating. This peptide stimulates cell attachment through the arginine-glycine-aspartic acid (RGD) sequence. Modification of medical implants with PepTite has been shown to promote ingrowth of surrounding cells into the material leading to better tissue integration, reduced inflammation and reduced fibrotic encapsulation. In this study, polyester and PTFE textiles were modified with PepTite. The effectiveness of this coating in enhancing wound healing was investigated in a simple vascular and cardiac valve model. Our results indicate that the RGD-containing peptide, PepTite, promoted the formation of an endothelial-like cell layer on both polyester and PTFE vascular patches in the dog model. PepTite was also found to promote the formation of a significantly thinner neointima (pannus) on polyester as compared to that on its uncoated control. These results were corroborated in the cardiac valve model in which a greater amount of thin pannus and less thrombus were seen on coated polyester sewing cuffs than on control uncoated cuffs. This research shows the promising tissue response to RGD coated textiles and the potential role of this peptide in material passivation via accelerated healing.
authors.affiliation: St. Jude Medical, Inc., St. Paul, MN 55117, USA.
authors.forename: K S
authors.initials: KS
authors.lastname: Tweden
authors.forename: H
authors.initials: H
authors.lastname: Harasaki
authors.forename: M
authors.initials: M
authors.lastname: Jones
authors.forename: J M
authors.initials: JM
authors.lastname: Blevitt
authors.forename: W S
authors.initials: WS
authors.lastname: Craig
authors.forename: M
authors.initials: M
authors.lastname: Pierschbacher
authors.forename: M N
authors.initials: MN
authors.lastname: Helmus
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D001807:Blood Vessel Prosthesis; D002448:Cell Adhesion / Q000187:drug effects; D004195:Disease Models, Animal; D004285:Dogs; D004727:Endothelium / Q000187:drug effects / Q000473:pathology; D005353:Fibronectins / Q000737:chemistry; D005355:Fibrosis; D006350:Heart Valve Prosthesis; D006801:Humans; D007249:Inflammation; D009842:Oligopeptides / Q000737:chemistry / Q000494:pharmacology; D011093:Polyethylene Terephthalates / Q000737:chemistry; D011138:Polytetrafluoroethylene / Q000737:chemistry; D011474:Prosthesis Design; D011971:Receptors, Immunologic; D013499:Surface Properties; D013501:Surface-Active Agents / Q000737:chemistry / Q000494:pharmacology; D013784:Textiles; D013927:Thrombosis / Q000517:prevention & control; D017539:Tunica Intima / Q000187:drug effects / Q000473:pathology; D014945:Wound Healing
chemical_list: D001672:Biocompatible Materials; D005353:Fibronectins; D009842:Oligopeptides; D011093:Polyethylene Terephthalates; D011971:Receptors, Immunologic; D013501:Surface-Active Agents; C047981:arginyl-glycyl-aspartic acid; D011138:Polytetrafluoroethylene