title: High expression cloning, purification, and assay of Ypt-GTPase-activating proteins.
authors.affiliation: Department of Molecular Genetics, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany.
authors.forename: P
authors.initials: P
authors.lastname: Vollmer
authors.forename: D
authors.initials: D
authors.lastname: Gallwitz
mesh_terms: D001345:Autoradiography / Q000379:methods; D002850:Chromatography, Gel / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000379:methods; D003001:Cloning, Molecular / Q000379:methods; D020558:GTP Phosphohydrolases / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism; D019204:GTP-Binding Proteins / Q000096:biosynthesis* / Q000302:isolation & purification* / Q000378:metabolism; D020690:GTPase-Activating Proteins; D005800:Genes, Fungal; D015698:Genomic Library; D006160:Guanosine Triphosphate / Q000378:metabolism; D007202:Indicators and Reagents; D010761:Phosphorus Radioisotopes; D014176:Protein Biosynthesis; D011506:Proteins / Q000302:isolation & purification* / Q000378:metabolism; D011865:Radioisotope Dilution Technique; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000302:isolation & purification / Q000378:metabolism; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*
chemical_list: D020690:GTPase-Activating Proteins; D007202:Indicators and Reagents; D010761:Phosphorus Radioisotopes; D011506:Proteins; D011994:Recombinant Proteins; D006160:Guanosine Triphosphate; D020558:GTP Phosphohydrolases; D019204:GTP-Binding Proteins