Intraocular levels of methotrexate after intravenous administration.
OBJECTIVE
To determine if adequate intraocular levels of methotrexate are achieved after intravenous administration.


METHODS
After intravenous administration, methotrexate levels were determined in the serum, the anterior chamber, and the cerebrospinal fluids of a patient with recurrent ocular lymphoma. A fluorescence polarization immunoassay was used to make the determinations.


RESULTS
At seven hours into a 24-hour intravenous infusion, methotrexate was at cytotoxic level in all samples. At 74 hours, cytotoxic levels were present only in the aqueous humor.


CONCLUSIONS
Sustained cytotoxic ocular methotrexate levels are achievable after systemic administration.
de Smet
M D
MD
Clinical Immunology Section, National Eye Institute, Bethesda, MD 20892-1858, USA.
Stark-Vancs
V
V
Kohler
D R
DR
Smith
J
J
Wittes
R
R
Nussenblatt
R B
RB
D000867:Anterior Chamber / Q000378:metabolism; D000964:Antimetabolites, Antineoplastic / Q000008:administration & dosage / Q000493:pharmacokinetics* / Q000627:therapeutic use; D001082:Aqueous Humor / Q000378:metabolism*; D001682:Biological Availability; D002555:Cerebrospinal Fluid / Q000378:metabolism; D005134:Eye Neoplasms / Q000188:drug therapy / Q000378:metabolism; D005260:Female; D016231:Fluorescence Polarization Immunoassay; D006801:Humans; D007262:Infusions, Intravenous; D008228:Lymphoma, Non-Hodgkin / Q000188:drug therapy / Q000378:metabolism; D008727:Methotrexate / Q000008:administration & dosage / Q000493:pharmacokinetics* / Q000627:therapeutic use; D008875:Middle Aged
D000964:Antimetabolites, Antineoplastic; D008727:Methotrexate