cDNA cloning of rat cardiotrophin-1 (CT-1): augmented expression of CT-1 gene in ventricle of genetically hypertensive rats.
Cardiotrophin-1 (CT-1) is a novel cytokine that was discovered from mouse embryoid bodies by means of expression cloning. It induces hypertrophy in cultured myocytes by activating a gp130 signaling pathway. To investigate the expression of the CT-1 gene in both normal adult and genetically hypertensive rats, we cloned rat CT-1 cDNA. The amino acid sequence is 94% identical to that of mouse CT-1. A significant amount of CT-1 mRNA was expressed in the ventricle of adult rats and also detected in the lung, kidney, liver, skeletal muscle, stomach and urinary bladder. Northern blot analysis revealed that the CT-1 mRNA level is significantly augmented in the ventricle of 12-week-old spontaneously hypertensive rats-stroke prone/Izm at a stage of established hypertension, suggesting a possible relevance of CT-1 to ventricular hypertrophy.
Ishikawa
M
M
Department of Medicine and Clinical Science, Kyoto University Graduate School of Medicine, Sakyo-ku, Japan.
Saito
Y
Y
Miyamoto
Y
Y
Kuwahara
K
K
Ogawa
E
E
Nakagawa
O
O
Harada
M
M
Masuda
I
I
Nakao
K
K
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D016887:Cardiopulmonary Resuscitation; D003001:Cloning, Molecular; D016207:Cytokines / Q000096:biosynthesis* / Q000235:genetics; D017931:DNA Primers; D018076:DNA, Complementary; D015870:Gene Expression; D006352:Heart Ventricles; D006973:Hypertension / Q000235:genetics* / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D009206:Myocardium / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D011918:Rats, Inbred SHR; D017208:Rats, Wistar; D011994:Recombinant Proteins / Q000096:biosynthesis; D017386:Sequence Homology, Amino Acid
D016207:Cytokines; D017931:DNA Primers; D018076:DNA, Complementary; D012333:RNA, Messenger; D011994:Recombinant Proteins; C092073:cardiotrophin 1