title: Inhibition of transcription starting from bacteriophage lambda pR promoter during the stringent response in Escherichia coli: implications for lambda DNA replication.
abstract: Replication of lambda plasmid DNA is halted in amino acid-starved wild type (stringent) strains whereas it proceeds in relA (relaxed) mutants. The only transcription which could be important in lambda plasmid DNA replication in amino acid-starved Escherichia coli cells is that starting from the pR promoter. Using a fusion which consists of the lacZ gene under the control of bacteriophage lambda pR promoter we found that transcription starting from this promoter was inhibited during the stringent, but not the relaxed, response in E. coli. We confirmed our conclusion by estimating the relative level of the pR transcript by RNA-DNA hybridization. We propose that decreased transcription from the pR promoter which serves as transcriptional activation of ori lambda is responsible for inhibition of lambda plasmid replication during the stringent response. The results presented in this paper, combined with our recent findings (published elsewhere), indicate that the transcriptional activation of ori lambda may be a main regulatory process controlling lambda DNA replication not only during the relaxed response but also in normal growth conditions.
authors.affiliation: Department of Molecular Biology, University of Gdańsk, Poland.
authors.forename: A
authors.initials: A
authors.lastname: Szalewska-Pałasz
authors.forename: G
authors.initials: G
authors.lastname: Wegrzyn
mesh_terms: D010582:Bacteriophage lambda / Q000235:genetics*; D004261:DNA Replication; D004279:DNA, Viral / Q000096:biosynthesis*; D004926:Escherichia coli / Q000235:genetics*; D007763:Lac Operon; D010957:Plasmids / Q000235:genetics*; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic
chemical_list: D004279:DNA, Viral