title: Shear activated channels in cell-attached patches of cultured bovine aortic endothelial cells.
abstract: We investigated the response of inward rectifier K+ (IRK) currents in bovine aortic endothelial cells (BAECs) to shear stress. Shear evoked reversible hyperpolarization in current clamped BAECs. Voltage clamped BAECs exhibited large inward and small outward whole cell K+ currents blocked by cesium and increased in amplitude by exposure to shear stress. The open state probability of IRK channels in cell-attached membrane patches was increased within minutes of exposure to shear stress. IRK channels in inside-out patches were activated by increases in [Ca2+]i from 10(-7) to 10(-6) mM. We demonstrate that shear stress induces hyperpolarization and gating of single channel and whole cell IRK currents in BAECs.
authors.affiliation: Medical College of Wisconsin, Department of Physiology and Medicine, MCW Clinic at Froedtert, 9200 West Wisconsin Avenue, Milwaukee, WI 53226, USA.
authors.forename: E R
authors.initials: ER
authors.lastname: Jacobs
authors.forename: C
authors.initials: C
authors.lastname: Cheliakine
authors.forename: D
authors.initials: D
authors.lastname: Gebremedhin
authors.forename: E K
authors.initials: EK
authors.lastname: Birks
authors.forename: P F
authors.initials: PF
authors.lastname: Davies
authors.forename: D R
authors.initials: DR
authors.lastname: Harder
mesh_terms: D000818:Animals; D001013:Aorta, Thoracic / Q000166:cytology / Q000502:physiology; D002118:Calcium / Q000502:physiology; D002417:Cattle; D002478:Cells, Cultured; D002586:Cesium / Q000494:pharmacology; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D015640:Ion Channel Gating / Q000187:drug effects / Q000502:physiology; D008564:Membrane Potentials / Q000502:physiology; D018408:Patch-Clamp Techniques; D015221:Potassium Channels / Q000502:physiology*
chemical_list: D015221:Potassium Channels; D002586:Cesium; D002118:Calcium