Cytogenetic effects of a food mutagen, 2-amino-1-methyl-6-phenylimidazo[4,5-beta]pyridine (PhIP), and its metabolite, 2-hydroxyamino-1-methy-6-phenylimidazo[4,5-beta]pyridine (N-OH-PhIP), on human and Chinese hamster cells in vitro.
2-Amino-1-methyl-6-phenylimidazo[4,5-beta]pyridine (PhIP) induced structural chromosomal aberrations (CAs) and sister-chromatid exchanges (SCEs) in human lymphocytes and human diploid fibroblasts (TIG-7) at concentrations above 12.5 microgram /ml in the presence of rat S9 mix. PhIP also elevated the frequencies of SCEs in human lymphocytes in the presence of rat S9 at concentrations above 2.0 microgram/ml with dose-dependency. A proximate form of metabolites of PhIP, 2-hydroxy-amino-1-methyl-6-phenylimidazo[4,5-beta]pyridine (N-OH-PhIP), caused CAs in human and Chinese hamster fibroblast cells in the absence of S9 mix at concentrations above 0.75 microgram/ml and 1.25 microgram/ml, respectively, which were 10 times lower than the effective concentration of PhIP. No marked differences were observed in the cytogenetic sensitivity to N-OH-PhIP between human and Chinese hamster cells, except between lymphocytes obtained from different donors.
Otsuka
C
C
Chromosome Research Center (CRC), Olympus Optical Co., Ltd., Tokyo, Japan.
Miura
K F
KF
Satoh
T
T
Hatanaka
M
M
Wakabayashi
K
K
Ishidate
M
M
D000328:Adult; D000818:Animals; D001711:Biotransformation; D016466:CHO Cells; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D006224:Cricetinae; D005260:Female; D005347:Fibroblasts; D006801:Humans; D007093:Imidazoles / Q000378:metabolism / Q000633:toxicity*; D008214:Lymphocytes; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity*; D011725:Pyridines / Q000633:toxicity*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012854:Sister Chromatid Exchange
D007093:Imidazoles; D009153:Mutagens; D011725:Pyridines; C060533:2-hydroxyamino-1-methyl-6-phenylimidazo(4,5-b)pyridine; C049584:2-amino-1-methyl-6-phenylimidazo(4,5-b)pyridine