Estimation of cholesterol sulphate in blood plasma and in erythrocyte membranes from individuals with Down's syndrome or diabetes mellitus type I.
OBJECTIVE
Plasma and erythrocyte membrane cholesterol sulphate (CS) were measured in patients suffering from diabetes and Down's syndrome.


METHODS
The procedure for separation and determination of CS comprised HPTLC (high-performance thin-layer chromatography) and densitometry.


RESULTS
The mean plasma and RBC membranes CS concentrations (+/- SD) of the control group (n = 16) was 188 +/- 47 micrograms/dL and 343 +/- 57 micrograms/10(12) RBC, respectively. In 15 patients with diabetes and 12 Down's syndrome patients substantially higher CS levels were found (diabetes: plasma-348 +/- 60 micrograms/dL; RBC membranes-646 +/- 113 micrograms/10(12) RBC; Down's syndrome: plasma-245 +/- 54 micrograms/dL; RBC membranes 427 +/- 74 micrograms/10(12) RBC). Analysis of variance and multiple comparison (Newman-Keuls test) show statistically significant differences between all samples both for erythrocytes, F(2.41) = 52.24, p < 0.05, and plasma, F(2.41) = 34.92, p < 0.05.


CONCLUSIONS
It is postulated that differences in CS levels may contribute to changes of erythrocyte properties in these pathological states.
Przybylska
M
M
Department of Medical Biophysics, University of Lodz, Poland.
Bryszewska
M
M
Nowicka
U
U
Szosland
K
K
Kedziora
J
J
Epand
R M
RM
D000328:Adult; D002788:Cholesterol Esters / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002855:Chromatography, Thin Layer / Q000379:methods; D003922:Diabetes Mellitus, Type 1 / Q000097:blood*; D004314:Down Syndrome / Q000097:blood*; D004910:Erythrocyte Membrane / Q000737:chemistry*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D015233:Models, Statistical; D010949:Plasma / Q000737:chemistry*; D012984:Software
D002788:Cholesterol Esters; C007045:cholesteryl sulfate