title: Regulation of SNM1, an inducible Saccharomyces cerevisiae gene required for repair of DNA cross-links.
abstract: The interstrand cross-link repair gene SNM1 of Saccharomyces cerevisiae was examined for regulation in response to DNA-damaging agents. Induction of SNM1-lacZ fusions was detected in response to nitrogen mustard, cis-platinum (II) diamine dichloride, UV light, and 8-methoxypsoralen + UVA, but not after heat-shock treatment or incubation with 2-dimethylaminoethylchloride, methylmethane sulfonate or 4-nitroquinoline-N-oxide. The promoter of SNM1 contains a 15 bp motif, which shows homology to the DRE2 box of the RAD2 promoter. Similar motifs have been found in promoter regions of other damage-inducible DNA repair genes. Deletion of this motif results in loss of inducibility of SNM1. Also, a putative negative upstream regulation sequence was found to be responsible for repression of constitutive transcription of SNM1. Surprisingly, no inducibility of SNM1 was found after treatment with DNA-damaging agents in strains without an intact DUN1 gene, while regulation seems unchanged in sad1 mutants.
authors.affiliation: Institut für Mikrobiologie, Abt. Biologie für Mediziner, J.W. Goethe-Universität, Frankfurt am Main, FRG.
authors.forename: R
authors.initials: R
authors.lastname: Wolter
authors.forename: W
authors.initials: W
authors.lastname: Siede
authors.forename: M
authors.initials: M
authors.lastname: Brendel
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology; D001483:Base Sequence; D002945:Cisplatin / Q000494:pharmacology; D016384:Consensus Sequence / Q000235:genetics; D003432:Cross-Linking Reagents / Q000737:chemistry / Q000494:pharmacology; D004249:DNA Damage; D004260:DNA Repair / Q000235:genetics*; D004271:DNA, Fungal / Q000187:drug effects / Q000528:radiation effects; D004268:DNA-Binding Proteins / Q000235:genetics*; D004706:Endodeoxyribonucleases; D005656:Fungal Proteins / Q000235:genetics*; D015966:Gene Expression Regulation, Fungal; D008730:Methoxsalen / Q000494:pharmacology; D008969:Molecular Sequence Data; D016296:Mutagenesis / Q000235:genetics; D009687:Nuclear Proteins; D011401:Promoter Regions, Genetic / Q000235:genetics; D011993:Recombinant Fusion Proteins; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D017384:Sequence Deletion; D012689:Sequence Homology, Nucleic Acid; D016385:TATA Box / Q000235:genetics; D014466:Ultraviolet Rays; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; D003432:Cross-Linking Reagents; D004271:DNA, Fungal; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D009687:Nuclear Proteins; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; D004706:Endodeoxyribonucleases; C090162:PSO2 protein, S cerevisiae; D001616:beta-Galactosidase; D002945:Cisplatin; D008730:Methoxsalen