Expression analysis of a sucrose synthase gene from sugar beet (Beta vulgaris L.).
To investigate the expression pattern of sucrose synthase, a cDNA from tap roots of sugar beet (Beta vulgaris L.) was isolated using a heterologous sucrose synthase cDNA from potato. The 2762 bp long cDNA clone designated SBSS 1 encodes for a 822 amino acid polypeptide of a predicted molecular mass of 93.7 kDa. The deduced amino acid sequence of sugar beet sucrose synthase has homologies of 65-70% when compared to predicted amino acid sequences of sucrose synthases from other species. RNA blot analysis shows that SBSS1 is expressed most predominant in tap root under normal growth conditions. Cold treatment and anaerobiosis lead to an increase in the steady-state levels of SBSS 1 mRNA in leaf and root tissue. In tap root slices, sugars in various concentrations had no influence on the SBSS 1 transcript level. On the other hand, wounding resulted in a decreased transcript level.
Hesse
H
H
Institut für Genbiologische Forschung Berlin, Germany.
Willmitzer
L
L
D000595:Amino Acid Sequence; D001483:Base Sequence; D002241:Carbohydrates / Q000494:pharmacology; D003001:Cloning, Molecular; D003080:Cold Temperature; D018076:DNA, Complementary; D004926:Escherichia coli / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D005964:Glucosyltransferases / Q000235:genetics*; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D014675:Vegetables / Q000201:enzymology*
D002241:Carbohydrates; D018076:DNA, Complementary; D012333:RNA, Messenger; D005964:Glucosyltransferases; C022699:sucrose synthase