Suppression of translation frameshift by upstream termination codon.
Retroviruses employ translation frameshift for gene expression. Translation frameshift takes place at the slippery heptanucleotide sequence on mRNA. A downstream stem-loop structure (and a pseudoknot formed by the stem-loop) enhances the level of ribosomal frameshifting. Here, we describe that an upstream termination codon suppresses the ribosomal frameshifting, leading to reduction in the expression of downstream genes.
Honda
A
A
Banyu Tsukuba Research Institute, Merck Research Laboratories, Ibarakgi, Japan. ayhonda@lab.nig.ac.jp.
Nishimura
S
S
D000595:Amino Acid Sequence; D001483:Base Sequence; D018388:Codon, Terminator; D004279:DNA, Viral; D016368:Frameshift Mutation; D015497:HIV-1 / Q000235:genetics; D008156:Luciferases / Q000235:genetics; D008324:Mammary Tumor Virus, Mouse / Q000235:genetics; D008969:Molecular Sequence Data; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics
D018388:Codon, Terminator; D004279:DNA, Viral; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D008156:Luciferases