Characterization of differentially expressed genes following brief cardiac ischemia.
For the study of ischemia-related adaptations we employed DDRT-PCR on mRNA obtained from heart regions undergoing brief (10') occlusions/reperfusions. We found and sequenced 52 differentially expressed clones that we further characterized using Northern analysis with RNA from a wide variety of tissues. We selected only clones (1) with a new sequence, (2) that showed a Northern signal, and (3) that exhibited organ-specific (heart, muscle) or (4) situation-specific (ischemia, non-specific stress) expression. We found two new heart-specific transcripts and three new stress-inducible genes. The transcripts with known sequences showed an expression pattern typical for repair processes after ischemia-reperfusion (ubiquitin, ATPases). About 20% of clones were truly differentially expressed.
Knöll
R
R
Department of Experimental Cardiology, Max-Planck-Institut, Bad Nauheim, Germany.
Zimmermann
R
R
Arras
M
M
Schaper
W
W
D000818:Animals; D015152:Blotting, Northern; D005786:Gene Expression Regulation / Q000502:physiology*; D006801:Humans; D008297:Male; D017202:Myocardial Ischemia / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013312:Stress, Physiological / Q000503:physiopathology; D013552:Swine
D012333:RNA, Messenger