Molecular characterization of the genes encoding pyruvate formate-lyase and its activating enzyme of Clostridium pasteurianum.
Formate is the major source of C1 units in many species of the genus Clostridium. In this study we have cloned and characterized the genes encoding pyruvate formate-lyase and its activating enzyme of Clostridium pasteurianum. The genetic and transcriptional organizations of the genes and the high level of homology exhibited by the respective gene products to their Escherichia coli counterparts indicate strong evolutionary conservation of these enzymes.
Weidner
G
G
Department of Microbiology and Genetics, University of Munich, Germany.
Sawers
G
G
D000123:Acetyltransferases / Q000235:genetics* / Q000276:immunology; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics* / Q000276:immunology; D001483:Base Sequence; D005075:Biological Evolution; D003001:Cloning, Molecular; D003013:Clostridium / Q000201:enzymology / Q000235:genetics* / Q000276:immunology; D017124:Conserved Sequence; D003429:Cross Reactions; D004789:Enzyme Activation; D004798:Enzymes / Q000235:genetics*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics / Q000276:immunology; D008969:Molecular Sequence Data; D012329:RNA, Bacterial / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D017385:Sequence Homology; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D004798:Enzymes; D012329:RNA, Bacterial; D012333:RNA, Messenger; D000123:Acetyltransferases; C040285:pyruvate formate-lyase activating enzyme; C022560:formate C-acetyltransferase