Determination of epitestosterone and testosterone in urine by high-performance liquid chromatography.
This paper describes an alternative HPLC method for the determination of testosterone and epitestosterone, which is simple, rapid, selective, sensitive and reproducible. Samples were prepared using a previous enzymatic hydrolysis with liquid-liquid extraction. The determination was carried out on a Hypersil BDS-C18 reversed-phase column with gradient elution and UV absorbance detection (240 nm). The limits of quantification (signal-to-noise ratio = 6) were 20 ng/ml for testosterone and 30 ng/ml for epitestosterone.
Navajas
R
R
Laboratorio de Control de Dopaje, Consejo Superior de Deportes, Madrid, Spain.
Imaz
C
C
Carreras
D
D
García
M
M
Pérez
M
M
Rodríguez
C
C
Rodríguez
A F
AF
Cortés
R
R
D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000706:statistics & numerical data; D004845:Epitestosterone / Q000652:urine*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013739:Testosterone / Q000652:urine*
D013739:Testosterone; D004845:Epitestosterone