A commercially available cell culture device modified for dentin barrier tests.
The suitability of a dentin barrier test based on a commercially available cell culture chamber was evaluated by testing the cytotoxicity of dental cements. The two chambers of the culture device as produced are separated by a membrane. This was replaced by a bovine dentin disk (500 micrometers thick). Mouse fibroblasts were grown on the "pulpal" side of the dentin for 24 h; test materials were then placed into the "cavity" side of the upper chamber. The number of viable cells was determined after 24 h. After exposure to zinc phosphate cement at a powder/liquid ratio of 2:1, approximately 100% of cells survived. A ratio of 1:1 yielded 81% survival. Only 24% and 28% of the cells survived after exposure to Ketac Fil and Ketac Silver, respectively. The light-curing glass ionomer cement (vitrebond) and zinc oxide-eugenol killed all cells. These results agree with those obtained from a previous study, wherein the dentin barrier test device was constructed in our laboratory.
Schmalz
G
G
Clinic of Operative Dentistry and Periodontology, University of Regensburg, Germany.
Garhammer
P
P
Schweiki
H
H
D000818:Animals; D002417:Cattle; D018929:Cell Culture Techniques / Q000295:instrumentation*; D002470:Cell Survival / Q000187:drug effects; D016722:Cermet Cements / Q000633:toxicity; D003738:Dental Cements / Q000633:toxicity*; D003782:Dental Pulp / Q000187:drug effects; D003804:Dentin / Q000502:physiology*; D003806:Dentin Permeability; D015194:Diffusion Chambers, Culture; D004867:Equipment Design; D005899:Glass Ionomer Cements / Q000633:toxicity; D007739:L Cells / Q000187:drug effects; D008298:Maleates / Q000633:toxicity; D051379:Mice; D012044:Regression Analysis; D018709:Statistics, Nonparametric; D018675:Toxicity Tests / Q000295:instrumentation; D015036:Zinc Phosphate Cement / Q000633:toxicity
D016722:Cermet Cements; D003738:Dental Cements; D005899:Glass Ionomer Cements; D008298:Maleates; C067790:Vitrabond; D015036:Zinc Phosphate Cement; C074226:Harvard Cement; C040964:Ketac-Fil