title: Defining the SOS operon of coliphage 186.
abstract: We have sequenced the LexA-controlled operon of coliphage 186 that carries the tum gene, whose product is necessary for UV induction of the 186 prophage. The operon consists of orf95 and orf97, and we have identified orf95 as the tum gene. The major translation products from orf95 result from internal initiations and modulate Tum activity. Tum is the product of the full-length Orf95 protein. The second gene of the operon, orf97, is of unknown function but, while it has little effect on prophage induction, its presence in the cell totally blocks infection of that cell by 186.
authors.affiliation: Department of Biochemistry, University of Adelaide, South Australia, Australia.
authors.forename: A M
authors.initials: AM
authors.lastname: Brumby
authors.forename: I
authors.initials: I
authors.lastname: Lamont
authors.forename: I B
authors.initials: IB
authors.lastname: Dodd
authors.forename: J B
authors.initials: JB
authors.lastname: Egan
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003090:Coliphages / Q000235:genetics*; D004279:DNA, Viral; D004926:Escherichia coli / Q000235:genetics / Q000821:virology; D005814:Genes, Viral; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D009876:Operon; D013014:SOS Response, Genetics; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D004279:DNA, Viral; D014764:Viral Proteins; C099333:orf97 protein, Enterobacteria phage 186; C099332:tum protein, Enterobacteria phage 186