title: Karyotyping human chromosomes by combinatorial multi-fluor FISH.
abstract: We have developed epifluorescence filter sets and computer software for the detection and discrimination of 27 different DNA probes hybridized simultaneously. For karyotype analysis, a pool of human chromosome painting probes, each labelled with a different fluor combination, was hybridized to metaphase chromosomes prepared from normal cells, clinical specimens, and neoplastic cell lines. Both simple and complex chromosomal rearrangements could be detected rapidly and unequivocally; many of the more complex chromosomal abnormalities could not be delineated by conventional cytogenetic banding techniques. Our data suggest that multiplex-fluorescence in situ hybridization (M-FISH) could have wide clinical utility and complement standard cytogenetics, particularly for the characterization of complex karyotypes.
authors.affiliation: Department of Genetics, Yale University School of Medicine, New Haven, Connecticut 06510, USA.
authors.forename: M R
authors.initials: MR
authors.lastname: Speicher
authors.forename: S
authors.initials: S
authors.lastname: Gwyn Ballard
authors.forename: D C
authors.initials: DC
authors.lastname: Ward
mesh_terms: D002869:Chromosome Aberrations / Q000235:genetics; D018244:Chromosomes, Artificial, Yeast; D002877:Chromosomes, Human / Q000235:genetics* / Q000648:ultrastructure; D015342:DNA Probes; D005260:Female; D005456:Fluorescent Dyes; D015321:Gene Rearrangement; D006801:Humans; D007091:Image Processing, Computer-Assisted; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D007621:Karyotyping / Q000379:methods*; D008297:Male; D012984:Software; D014407:Tumor Cells, Cultured
chemical_list: D015342:DNA Probes; D005456:Fluorescent Dyes