HIV-1 entry cofactor: functional cDNA cloning of a seven-transmembrane, G protein-coupled receptor.
A cofactor for HIV-1 (human immunodeficiency virus-type 1) fusion and entry was identified with the use of a novel functional complementary DNA (cDNA) cloning strategy. This protein, designated "fusin," is a putative G protein-coupled receptor with seven transmembrane segments. Recombinant fusin enabled CD4-expressing nonhuman cell types to support HIV-1 Env-mediated cell fusion and HIV-1 infection. Antibodies to fusin blocked cell fusion and infection with normal CD4-positive human target cells. Fusin messenger RNA levels correlated with HIV-1 permissiveness in diverse human cell types. Fusin acted preferentially for T cell line-tropic isolates, in comparison to its activity with macrophagetropic HIV-1 isolates.
Feng
Y
Y
Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases (NIAID), NIH, Bethesda, MD 20892, USA.
Broder
C C
CC
Kennedy
P E
PE
Berger
E A
EA
D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D015704:CD4 Antigens / Q000502:physiology*; D015496:CD4-Positive T-Lymphocytes / Q000821:virology; D002460:Cell Line; D002462:Cell Membrane / Q000821:virology; D018925:Chemokines / Q000502:physiology; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004195:Disease Models, Animal; D019204:GTP-Binding Proteins / Q000378:metabolism; D015726:Giant Cells; D015699:HIV Envelope Protein gp120 / Q000502:physiology; D015497:HIV-1 / Q000472:pathogenicity* / Q000502:physiology; D006367:HeLa Cells; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000821:virology; D008264:Macrophages / Q000821:virology; D008561:Membrane Fusion; D008565:Membrane Proteins / Q000235:genetics / Q000502:physiology*; D051379:Mice; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000378:metabolism; D019718:Receptors, CXCR4; D011994:Recombinant Proteins; D014162:Transfection
D015704:CD4 Antigens; D018925:Chemokines; D018076:DNA, Complementary; D015699:HIV Envelope Protein gp120; D008565:Membrane Proteins; D012333:RNA, Messenger; D019718:Receptors, CXCR4; D011994:Recombinant Proteins; D019204:GTP-Binding Proteins