Characterization of two class II chitinase genes from peanut and expression studies in transgenic tobacco plants.
Two different genes encoding class II chitinases from peanut (Arachis hypogaea L. cv. NC4), A.h.Chi2;1 and A.h.Chi2;2, have been cloned. In peanut cell suspension cultures, mRNA levels of A.h.Chi2;2 increased after ethylene or salicylate treatment and in the presence of conidia from Botrytis cinerea. The second gene, A.h.Chi2;1, was only expressed after treatment with the fungal spores. Transgenic tobacco plants containing the complete peanut A.h.Chi2;1 gene exhibited essentially the same expression pattern in leaves as observed in peanut cell cultures. Expression characteristics of transgenic tobacco carrying a promoter-GUS fusion of A.h.Chi2;1 are described.
Kellmann
J W
JW
Max-Planck-Institut für Züchtungsforschung, Köln, FRG.
Kleinow
T
T
Engelhardt
K
K
Philipp
C
C
Wegener
D
D
Schell
J
J
Schreier
P H
PH
D000595:Amino Acid Sequence; D010367:Arachis / Q000201:enzymology / Q000235:genetics* / Q000382:microbiology; D001483:Base Sequence; D002688:Chitinases / Q000096:biosynthesis / Q000145:classification / Q000235:genetics*; D005030:Ethylenes / Q000494:pharmacology; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D017930:Genes, Reporter; D015698:Genomic Library; D003904:Mitosporic Fungi / Q000472:pathogenicity; D008969:Molecular Sequence Data; D010937:Plant Growth Regulators / Q000494:pharmacology; D010940:Plant Proteins / Q000096:biosynthesis / Q000145:classification / Q000235:genetics*; D030821:Plants, Genetically Modified; D010947:Plants, Toxic; D011994:Recombinant Proteins / Q000096:biosynthesis; D012459:Salicylates / Q000494:pharmacology; D020156:Salicylic Acid; D017386:Sequence Homology, Amino Acid; D014026:Nicotiana / Q000235:genetics
D005030:Ethylenes; D010937:Plant Growth Regulators; D010940:Plant Proteins; D011994:Recombinant Proteins; D012459:Salicylates; C053376:pathogenesis-related proteins, plant; C036216:ethylene; C099227:CHI2,2 protein, Arachis hypogaea; D002688:Chitinases; C099226:chi2-1 protein, Arachis hypogaea; D020156:Salicylic Acid