title: Agalactosyl IgG in aggregates from the rheumatoid joint.
abstract: It has been postulated that agalactosyl immunoglobulin G (IgG) self-associates to form pathological aggregates in the rheumatoid joint. To examine this hypothesis, IgG aggregates from synovial fluid (SF) of 22 patients with RA were prepared by precipitation with polyethylene glycol (PEG) 6000. The PEG precipitates and SFs were reduced with 2-mercaptoethanol (2ME) and bound to protein G. This procedure isolated the IgG in the PEG precipitates from other contaminating glycosylated proteins. The levels of galactose and N-acetylglucosamine (GlcNAc) residues present on the reduced IgG were quantified by their ability to bind the lectins Ricinus communis (RCA)120 and Bandeiraea simplicifolia (BS) II. Proportionally less galactose (expressed as a ratio of bound RCA120 to BS II) was present on the IgG from the PEG precipitates than on the IgG in the paired SF (P = 0.001). However, in many cases more RCA120 as well as BS II bound to IgG from PEG precipitates than from the corresponding SF. It is considered that agalactosyl IgG occurs preferentially in RA SF PEG precipitates and that this IgG may also exhibit increased Fab glycosylation.
authors.affiliation: Department of Pathology and Microbiology, School of Medical Sciences, Bristol.
authors.forename: K A
authors.initials: KA
authors.lastname: Leader
authors.forename: G C
authors.initials: GC
authors.lastname: Lastra
authors.forename: J R
authors.initials: JR
authors.lastname: Kirwan
authors.forename: C J
authors.initials: CJ
authors.lastname: Elson
mesh_terms: D001172:Arthritis, Rheumatoid / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000302:isolation & purification*; D037102:Lectins; D011092:Polyethylene Glycols; D013582:Synovial Fluid / Q000276:immunology*
chemical_list: D007074:Immunoglobulin G; D037102:Lectins; C064155:agalactosyl IGG; D011092:Polyethylene Glycols