Microwave-enhanced immunohistochemical staining of a formalin-sensitive antigen-laminin receptor.
Many potentially useful antigens have been difficult to detect in formalin-fixed, paraffin-embedded tissues. Recently a number of pathological and research laboratories have demonstrated that some antigens masked by formalin fixation could be restored to detectability by microwave heating. Previously, we were unable to demonstrate laminin receptor in cells processed by the routine fixation. Our results showed that microwave heating together with trypsin produced the best immunohistochemical staining for this receptor. Nevertheless, no significance was found in the levels of 67 kD LR in high and low metastatic tumor cell lines.
Chew
E C
EC
Department of Anatomy, Chinese University of Hong Kong, Shatin, N.T.
Wu
S
S
Yam
H F
HF
Yang
L
L
Cheng-Chew
S B
SB
Zheng
J
J
Wu
B Q
BQ
D000230:Adenocarcinoma / Q000737:chemistry / Q000473:pathology / Q000648:ultrastructure; D000951:Antigens, Neoplasm / Q000032:analysis*; D018286:Carcinoma, Giant Cell / Q000737:chemistry / Q000473:pathology / Q000648:ultrastructure; D005557:Formaldehyde; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008175:Lung Neoplasms / Q000737:chemistry / Q000473:pathology / Q000648:ultrastructure; D008872:Microwaves; D016612:Paraffin Embedding; D017474:Receptors, Laminin / Q000032:analysis*; D012680:Sensitivity and Specificity; D016707:Tissue Fixation; D014357:Trypsin / Q000378:metabolism; D014407:Tumor Cells, Cultured
D000951:Antigens, Neoplasm; D017474:Receptors, Laminin; D005557:Formaldehyde; D014357:Trypsin