Sp1 is critical for basal and E2-transactivated transcription from the bovine papillomavirus type 1 P89 promoter.
The bovine papillomavirus type 1 (BPV-1) long control region (LCR) contains at least three consensus binding sites for the transcription factor Sp1 at nucleotides (nt) 7800, 7833 and 7854. A high basal-level P89 expression vector consisting of an origin-deleted LCR fused to the chloramphenicol acetyltransferase (CAT) gene was utilized to determine the role of these Sp1 sites in the regulation of transcription from the BPV-1 P89 promoter. The three Sp1 sites were capable of binding Sp1 in vitro. Mutation of these sites in the background of the origin-deleted LCR-CAT or a wild-type LCR-CAT construct resulted in decreased basal expression from P89. In addition, mutation of the Sp1 sites in the wild-type background caused a reduction in E2-transactivation potential. These data illustrate the importance of these Sp1 sites in regulating both basal and E2-transactivated P89 expression.
Sandler
A B
AB
Laboratory of Tumor Virus Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA.
Baker
C C
CC
Spalholz
B A
BA
D000818:Animals; D001483:Base Sequence; D052688:Bovine papillomavirus 1 / Q000235:genetics*; D002417:Cattle; D004268:DNA-Binding Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D016329:Sp1 Transcription Factor / Q000502:physiology*; D015533:Transcriptional Activation; D014764:Viral Proteins / Q000235:genetics*
D004268:DNA-Binding Proteins; C051550:E2 protein, Bovine papillomavirus; D016329:Sp1 Transcription Factor; D014764:Viral Proteins