title: GSG1, a yeast gene required for sporulation.
abstract: We have identified a gene, GSG1 (general sporulation gene 1), required for sporulation in Saccharomyces cerevisiae. Diploids homozygous for a disruption of GSG1 fail to sporulate. The gene has an open reading frame of 2094 bp, encoding a polypeptide with an expected size of 77 kDa. GSG1 is expressed mitotically in both a and alpha haploids, and both mitotically and meiotically in diploids. The message level of GSG1 increases approximately two-fold after 4-6 h of sporulation. gsg1 mutants enter pre-meiotic DNA synthesis later than wild-type diploids. Mutant diploids are not rescued by spo13. These results suggest that GSG1 has a role late in meiosis following DNA replication.
authors.affiliation: Department of Biochemistry, University of Minnesota, Minneapolis 55455, USA.
authors.forename: M D
authors.initials: MD
authors.lastname: Kaytor
authors.forename: D M
authors.initials: DM
authors.lastname: Livingston
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000737:chemistry; D005800:Genes, Fungal / Q000502:physiology*; D008969:Molecular Sequence Data; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D013172:Spores, Fungal / Q000235:genetics* / Q000502:physiology
chemical_list: D004271:DNA, Fungal