The yeast CDC16 and CDC27 genes restrict DNA replication to once per cell cycle.
CDC16 and CDC27 were identified as genes in S. cerevisiae necessary to limit DNA replication to once per cell cycle. A screen for mutants that overreplicated their DNA uncovered new conditional alleles that cause accumulation of up to 8C DNA. DNA overreplication involves all chromosomes and does not require passage through mitosis or another START. It occurs within a single cell cycle and can cause arrest at the MEC1 checkpoint. Remarkably, Clb2-Cdc28 activity remains elevated in the overreplicating cells. These observations distinguish CDC16 and CDC27 from other mutants that accumulate extra DNA after completing an aberrent mitosis, or skipping mitosis altogether, and entering a second, inappropriate G1 and S phase. CDC16 and CDC27 may contribute to replication control by targeted proteolysis of an S phase initiator.
Heichman
K A
KA
Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington 98104, USA.
Roberts
J M
JM
D064191:Apc3 Subunit, Anaphase-Promoting Complex-Cyclosome; D064194:Apc6 Subunit, Anaphase-Promoting Complex-Cyclosome; D016203:CDC2 Protein Kinase / Q000235:genetics*; D017489:CDC28 Protein Kinase, S cerevisiae / Q000378:metabolism; D002452:Cell Count; D002453:Cell Cycle / Q000235:genetics; D018797:Cell Cycle Proteins / Q000235:genetics*; D004261:DNA Replication / Q000235:genetics / Q000502:physiology*; D004271:DNA, Fungal / Q000502:physiology; D016521:Electrophoresis, Gel, Pulsed-Field; D016195:G2 Phase / Q000235:genetics; D005800:Genes, Fungal / Q000502:physiology*; D008938:Mitosis / Q000235:genetics; D009154:Mutation / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000201:enzymology / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D013696:Temperature; D044767:Ubiquitin-Protein Ligases
D064191:Apc3 Subunit, Anaphase-Promoting Complex-Cyclosome; D064194:Apc6 Subunit, Anaphase-Promoting Complex-Cyclosome; C577397:CDC16 protein, S cerevisiae; C577355:CDC27 protein, S cerevisiae; D018797:Cell Cycle Proteins; D004271:DNA, Fungal; D029701:Saccharomyces cerevisiae Proteins; D044767:Ubiquitin-Protein Ligases; D016203:CDC2 Protein Kinase; D017489:CDC28 Protein Kinase, S cerevisiae