Structure of a D-protein gene and amino-acid sequences of the highly repetitive D-proteins secreted by the accessory glands of the mealworm beetle.
The D-group proteins form the major component of the proteinaceous secretion of the tubular accessory glands of the yellow mealworm beetle, Tenebrio molitor. In a previous paper, we reported the sequence of two D-protein cDNAs and their inferred translation products. Both proteins contain three highly repetitive domains (A, A' and B). In this paper, we present the cDNA-inferred sequences of 8 more D-proteins, none of which contains an A' domain. We also present the structure of a D-protein gene. Southern analysis suggests that genes coding for an A' domain are relatively rare. Genes with a total of 7 or 8 (A + B domain) repeats seem most common.
Paesen
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GC
Department of Zoology, University of Vermont, Burlington 05405-0086, USA.
Feng
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Happ
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GM
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D017931:DNA Primers; D015342:DNA Probes; D018076:DNA, Complementary; D017344:Genes, Insect / Q000235:genetics*; D019476:Insect Proteins; D008969:Molecular Sequence Data; D011506:Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics; D013712:Tenebrio / Q000235:genetics*
C099261:D1 protein, Tenebrio molitor; C099262:D2 protein, Tenebrio molitor; D017931:DNA Primers; D015342:DNA Probes; D018076:DNA, Complementary; D019476:Insect Proteins; D011506:Proteins