Gene expression in response to retinoic acid in novel human chromosome 21 monochromosomal cell hybrids.
To access a wide a variety of expressed sequence from human chromosome 21 we have placed this chromosome into undifferentiated P19 mouse embryonic carcinoma cells. Cell lines resulting from these experiments have a range of morphologies and a wide variety of karyotypes. We have studied the retinoic acid response of five cell lines, compared to P19 cells, by observing three markers of retinoic acid induced P19 differentiation--cell morphology, RAR alpha and Wnt1 transcription. We see an 'early' retinoic acid response effect, however this response breaks down by the time the 'late' gene. Wnt1 would be transcribed in P19 cells. A highly responsive cell line will be useful for cloning expressed sequences from human chromosome 21 which are produced by early genes in retinoic acid inducible pathways, such as those involved in neurogenesis.
Yulug
I G
IG
Department of Biochemistry and Molecular Genetics, Imperial College School of Medicine at St. Mary's, London, United Kingdom.
Killary
A M
AM
Sandhu
A K
AK
Athwal
R S
RS
Fox
M
M
Fisher
E M
EM
D000818:Animals; D001483:Base Sequence; D018236:Carcinoma, Embryonal; D002459:Cell Fusion; D002460:Cell Line; D002886:Chromosomes, Human, Pair 17; D017931:DNA Primers; D015870:Gene Expression / Q000187:drug effects*; D006801:Humans; D006822:Hybrid Cells; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011505:Protein-Tyrosine Kinases / Q000096:biosynthesis; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis; D018168:Receptors, Retinoic Acid / Q000096:biosynthesis; D011994:Recombinant Proteins / Q000096:biosynthesis; D000072482:Retinoic Acid Receptor alpha; D014158:Transcription, Genetic; D014212:Tretinoin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured; D051153:Wnt Proteins; D051155:Wnt1 Protein; D029961:Zebrafish Proteins
D017931:DNA Primers; D011518:Proto-Oncogene Proteins; C000607071:RARA protein, human; C000607072:Rara protein, mouse; D018168:Receptors, Retinoic Acid; D011994:Recombinant Proteins; D000072482:Retinoic Acid Receptor alpha; C495247:WNT1 protein, human; D051153:Wnt Proteins; D051155:Wnt1 Protein; C495246:Wnt1 protein, mouse; D029961:Zebrafish Proteins; D014212:Tretinoin; D011505:Protein-Tyrosine Kinases