Induction of a novel cytochrome P450 (CYP93 family) by methyl jasmonate in soybean suspension-cultured cells.
We isolated a cDNA encoding a novel cytochrome P450 (CYP93A1) from soybean suspension-cultured cells that had been treated with methyl jasmonate (MeJA). The amino acid sequence of the gene product had 30-40% identity with those of other plant P450s. The protein contained the heme-binding domain which is highly conserved among plant P450s. Transcription of the cytochrome P450 gene in soybean cells was induced by 30 microM MeJA even in the presence of cycloheximide, and reached maximum level 6 h after MeJA treatment. This is the first report of a plant cytochrome P450 gene whose transcription is induced by MeJA even without protein synthesis.
Suzuki
G
G
Department of Biological Sciences, Faculty of Bioscience and Biotechnology, Yokohama, Japan.
Ohta
H
H
Kato
T
T
Igarashi
T
T
Sakai
F
F
Shibata
D
D
Takano
A
A
Masuda
T
T
Shioi
Y
Y
Takamiya
K
K
D000085:Acetates / Q000494:pharmacology*; D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D002478:Cells, Cultured; D003001:Cloning, Molecular; D003517:Cyclopentanes / Q000494:pharmacology*; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D015342:DNA Probes; D004790:Enzyme Induction; D017343:Genes, Plant; D006899:Mixed Function Oxygenases; D008969:Molecular Sequence Data; D054883:Oxylipins; D010937:Plant Growth Regulators / Q000494:pharmacology*; D010940:Plant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D016415:Sequence Alignment; D030262:Soybean Proteins; D013025:Glycine max / Q000235:genetics*
D000085:Acetates; D003517:Cyclopentanes; D015342:DNA Probes; D054883:Oxylipins; D010937:Plant Growth Regulators; D010940:Plant Proteins; D030262:Soybean Proteins; C072239:methyl jasmonate; D003577:Cytochrome P-450 Enzyme System; D006899:Mixed Function Oxygenases; C042062:cytochrome P450 CYP93A1 (Glycine max)