title: Antioxidant activity of natural flavonoids is governed by number and location of their aromatic hydroxyl groups.
abstract: We studied antioxidant activity of five natural flavonoids in canola oil, an emulsion, and rat red blood cell membrane. In the canola oil heated at 105 degrees C, myricetin most effectively attenuated lipid oxidation, followed by quercetin, morin and kaempferol in a decreasing order. When the emulsion containing canola oil was incubated at 54 degrees C, quercetin was most protective against lipid oxidation, followed by kaempferol, myricetin and morin in a decreasing rank. Apigenin demonstrated no antioxidant activity in either the canola oil or the emulsion. Of the flavonoids tested, quercetin was the best in quenching free radical chain reaction in rat red blood cell membrane. The antioxidative characteristics of these flavonoids was determined by multiple factors including the system used, the hydrophobicity, and the total number and the location of aromatic hydroxyl groups. We conclude that myricetin is an effective antioxidant in canola oil, while quercetin is an effective protector against lipid oxidation in the cell membrane.
authors.affiliation: Department of Biochemistry, Chinese University of Hong Kong, Shatin, New Territories, Hong Kong.
authors.forename: Z Y
authors.initials: ZY
authors.lastname: Chen
authors.forename: P T
authors.initials: PT
authors.lastname: Chan
authors.forename: K Y
authors.initials: KY
authors.lastname: Ho
authors.forename: K P
authors.initials: KP
authors.lastname: Fung
authors.forename: J
authors.initials: J
authors.lastname: Wang
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry* / Q000494:pharmacology*; D004655:Emulsions; D004910:Erythrocyte Membrane / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D005229:Fatty Acids, Monounsaturated; D005419:Flavonoids / Q000737:chemistry* / Q000494:pharmacology*; D005609:Free Radicals; D006461:Hemolysis / Q000187:drug effects; D006900:Hydroxylation; D066298:In Vitro Techniques; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D011794:Quercetin / Q000737:chemistry / Q000494:pharmacology; D000074262:Rapeseed Oil; D051381:Rats; D017207:Rats, Sprague-Dawley; D013329:Structure-Activity Relationship
chemical_list: D000975:Antioxidants; D004655:Emulsions; D005229:Fatty Acids, Monounsaturated; D005419:Flavonoids; D005609:Free Radicals; D000074262:Rapeseed Oil; C040015:myricetin; D011794:Quercetin