title: Association of antineutrophil cytoplasmic antibodies with resistance to treatment of left-sided ulcerative colitis: results of a pilot study.
abstract: OBJECTIVE
  To determine the frequency of antineutrophil cytoplasmic antibodies with perinuclear staining in patients with treatment-resistant left-sided ulcerative colitis.
  
  
  METHODS
  We studied four groups: treatment-resistant left-sided ulcerative colitis, treatment-responsive left-sided ulcerative colitis, ulcerative colitis historical controls, and healthy control subjects. Antineutrophil cytoplasmic antibodies were detected by enzyme-linked immunosorbent assay, and positive results were confirmed by demonstration of a perinuclear staining pattern by indirect immunofluorescence assay.
  
  
  RESULTS
  The frequency of perinuclear antineutrophil cytoplasmic antibodies was significantly greater in treatment-resistant left-sided ulcerative colitis (90%) than in treatment-responsive left-sided ulcerative colitis (62%) (P = 0.03) or in ulcerative colitis historical controls (60%).
  
  
  CONCLUSIONS
  The increased frequency of perinuclear antineutrophil cytoplasmic antibodies in treatment-resistant left-sided ulcerative colitis suggests a possible association between these antibodies and relative resistance to medical therapy in patients with ulcerative colitis.
authors.affiliation: Inflammatory Bowel Disease Clinic, Mayo Clinic Rochester, Minnesota 55905, USA.
authors.forename: W J
authors.initials: WJ
authors.lastname: Sandborn
authors.forename: C J
authors.initials: CJ
authors.lastname: Landers
authors.forename: W J
authors.initials: WJ
authors.lastname: Tremaine
authors.forename: S R
authors.initials: SR
authors.lastname: Targan
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D019268:Antibodies, Antineutrophil Cytoplasmic; D001323:Autoantibodies / Q000032:analysis*; D015415:Biomarkers / Q000032:analysis; D003093:Colitis, Ulcerative / Q000188:drug therapy / Q000276:immunology*; D003593:Cytoplasm / Q000276:immunology; D004351:Drug Resistance; D004797:Enzyme-Linked Immunosorbent Assay; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D008875:Middle Aged; D010865:Pilot Projects; D012189:Retrospective Studies; D017211:Treatment Failure
chemical_list: D019268:Antibodies, Antineutrophil Cytoplasmic; D001323:Autoantibodies; D015415:Biomarkers