Identification and characterization of specific receptor for interleukin-8 from the surface of human monocytes.
A specific receptor for interleukin-8 has been identified on the surface of human monocytes using 125I IL-8 as a probe. A binding kinetic pattern shows that saturation was attained after 90 min and that the receptor was distinct from the receptors of other cytokines (IL-L alpha, IL-2, TNF alpha, GMCSF) and FMLP. Scatchard analysis of the binding data shows that 7000-10,000 receptors /monocyte are present with an equilibrium Kd 7 x 10(-9) M. By immunoblot, the receptor for IL-8 showed a sharp band with approximate M.W. 59 kD, consistent with the M.W. of IL-8 receptor of neutrophils. In Boyden Chamber, monocytes migrated towards IL-8 and the cytokine was observed to induce transient rise of intracellular Ca+2 in the cells. Thus, identification of functionally active IL-8 receptor in monocyte may be helpful for understanding its possible role during inflammation.
Bishayi
B
B
Indian Institute of Chemical Biology, Calcutta, India.
Samanta
A K
AK
D015703:Antigens, CD / Q000032:analysis* / Q000378:metabolism*; D001665:Binding Sites; D002118:Calcium / Q000378:metabolism; D002633:Chemotaxis; D006801:Humans; D015151:Immunoblotting; D007457:Iodine Radioisotopes; D009000:Monocytes / Q000737:chemistry*; D018123:Receptors, Interleukin / Q000032:analysis* / Q000378:metabolism*; D023062:Receptors, Interleukin-8A
D015703:Antigens, CD; D007457:Iodine Radioisotopes; D018123:Receptors, Interleukin; D023062:Receptors, Interleukin-8A; D002118:Calcium