title: Pregnancy and birth after intracytoplasmic sperm injection of in vitro matured germinal-vesicle stage oocytes: case report.
abstract: OBJECTIVE
  To report a normal pregnancy and the delivery of a healthy child after the combination of in vitro maturation of germinal-vesicle stage oocytes and intracytoplasmic sperm injection (ICSI) in a patient.
  
  
  METHODS
  Procedures were performed in a tertiary IVF center coupled with an institutional research environment.
  
  
  METHODS
  Maturation rate of immature oocytes after in vitro maturation and intactness, fertilization, and developmental rates of oocytes after microinjection.
  
  
  RESULTS
  Nine of 14 germinal-vesicle stage oocytes matured to the metaphase II stage after 30 hours of in vitro culture (64%). Seven of eight injected and intact oocytes fertilized normally (78%) and five of them cleaved with < 20% fragmentation (71%). Four embryos were transferred and a singleton pregnancy was obtained that ended in the delivery of a healthy child.
  
  
  CONCLUSIONS
  In vitro maturation of immature oocytes together with ICSI can result in normal fertilization, embryo development, pregnancy, and the delivery of healthy child.
authors.affiliation: Centre for Reproductive Medicine, University Hospital, Vrije Universiteit Brussel, Belgium.
authors.forename: Z P
authors.initials: ZP
authors.lastname: Nagy
authors.forename: J
authors.initials: J
authors.lastname: Cecile
authors.forename: J
authors.initials: J
authors.lastname: Liu
authors.forename: A
authors.initials: A
authors.lastname: Loccufier
authors.forename: P
authors.initials: P
authors.lastname: Devroey
authors.forename: A
authors.initials: A
authors.lastname: Van Steirteghem
mesh_terms: D000328:Adult; D046508:Culture Techniques; D003593:Cytoplasm; D004624:Embryo Transfer; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D006801:Humans; D007248:Infertility, Male / Q000628:therapy*; D008297:Male; D008845:Microinjections; D009865:Oocytes / Q000502:physiology*; D010062:Ovulation Induction; D011247:Pregnancy; D011256:Pregnancy Outcome