Chronic in vivo sodium azide infusion induces selective and stable inhibition of cytochrome c oxidase.
The effect of chronic subcutaneous infusion of sodium azide on the activity of mitochondrial respiratory chain enzymes was investigated in Sprague-Dawley rats. Treatment with approximately 1 mg/kg/h sodium azide induced chronic, partial inhibition of cytochrome c oxidase, whereas the activities of respiratory complexes I and III were not significantly affected. The inhibition of cytochrome c oxidase was evident by 7 days after infusion began, and the effect was stable for at least 3 weeks. The selectivity of azide for cytochrome c oxidase is discussed in the context of other findings of azide effects on enzymes. The results of the present study indicate that the sodium azide infusion paradigm described here provides a useful tool for the evaluation of selective and stable cytochrome oxidase inhibition in vivo.
Bennett
M C
MC
National Institute on Aging, Laboratory of Neurosciences, National Institutes of Health, Bethesda, MD 20892, USA.
Mlady
G W
GW
Kwon
Y H
YH
Rose
G M
GM
D000704:Analysis of Variance; D000818:Animals; D001386:Azides / Q000494:pharmacology*; D001921:Brain / Q000187:drug effects / Q000201:enzymology / Q000648:ultrastructure; D003576:Electron Transport Complex IV / Q000037:antagonists & inhibitors*; D008297:Male; D008928:Mitochondria / Q000201:enzymology; D009153:Mutagens / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D019810:Sodium Azide; D013997:Time Factors
D001386:Azides; D009153:Mutagens; D019810:Sodium Azide; D003576:Electron Transport Complex IV