title: New isochromophilones VII and VIII produced by Penicillium sp. FO-4164.
abstract: New isochromophilones VII and VIII were isolated from the culture broth of Penicillium sp. FO-4164. The structures were elucidated as 6H-2-benzopyran-6,8(7H)-dione, 5-chloro-3-(3',5'-dimethyl-1',3'-heptadienyl)-1,7,8a-trihydro-7, 8a-dihydroxy-7-methyl-7-acetate for isochromophilone VII and 6H-2-benzopyran-6-one,5-chloro-3-(3',5'-dimethyl-1', 3'-heptadienyl)-1,7,8,8a-tetrahydro-7,8-dihydroxy-7-methyl-8-acetate for isochromophilone VIII. Isochromophilones VII and VIII inhibited diacylglycerol acyltransferase activity with IC50 values of 20.0 and 127 microM and acyl-CoA: cholesterol acyltransferase activity with IC50 values of 24.5 and 47.0 microM, respectively.
authors.affiliation: Research Center for Biological Function, The Kitasato Institute, Tokyo, Japan.
authors.forename: D J
authors.initials: DJ
authors.lastname: Yang
authors.forename: H
authors.initials: H
authors.lastname: Tomoda
authors.forename: N
authors.initials: N
authors.lastname: Tabata
authors.forename: R
authors.initials: R
authors.lastname: Masuma
authors.forename: S
authors.initials: S
authors.lastname: Omura
mesh_terms: D000217:Acyltransferases / Q000037:antagonists & inhibitors*; D000818:Animals; D001578:Benzopyrans / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D051048:Diacylglycerol O-Acyltransferase; D004791:Enzyme Inhibitors / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D005285:Fermentation; D066298:In Vitro Techniques; D009682:Magnetic Resonance Spectroscopy; D008826:Microbial Sensitivity Tests; D008862:Microsomes, Liver / Q000201:enzymology; D015394:Molecular Structure; D010407:Penicillium / Q000145:classification / Q000378:metabolism*; D051381:Rats; D013237:Stereoisomerism; D002785:Sterol O-Acyltransferase / Q000037:antagonists & inhibitors*
chemical_list: D001578:Benzopyrans; D004791:Enzyme Inhibitors; C099292:isochromophilone VII; C099293:isochromophilone VIII; D000217:Acyltransferases; D051048:Diacylglycerol O-Acyltransferase; D002785:Sterol O-Acyltransferase