Simultaneous determination of zolpidem and zopiclone in human plasma by gas chromatography-nitrogen-phosphorus detection.
A simple, specific and selective method for the simultaneous determination of zolpidem and zopiclone in human plasma is described. After a liquid-liquid extraction, the extract is injected into a capillary gas chromatograph with an OV-1 fused-silica column coupled to a nitrogen-phosphorus detector. The detection limits are 1 and 2 ng/ml for zolpidem and zopiclone, respectively. The method described is reproducible and linear over a range of concentrations, rendering it suitable for use for pharmacokinetic studies or toxicological evaluations. Absolute identification of the chromatographed compounds is accomplished by gas chromatography--mass spectrometry in both electron-impact and positive-ion chemical ionisation modes.
Stanke
F
F
Laboratoire de Pharmacologie, Centre Hospitalier Universitaire de Grenoble, France.
Jourdil
N
N
Bessard
J
J
Bessard
G
G
D016477:Artifacts; D053961:Azabicyclo Compounds; D002849:Chromatography, Gas / Q000379:methods*; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D006993:Hypnotics and Sedatives / Q000097:blood*; D009584:Nitrogen; D010758:Phosphorus; D010879:Piperazines / Q000097:blood*; D011725:Pyridines / Q000097:blood*; D015203:Reproducibility of Results; D000077334:Zolpidem
D053961:Azabicyclo Compounds; D006993:Hypnotics and Sedatives; D010879:Piperazines; D011725:Pyridines; C515050:zopiclone; D010758:Phosphorus; D000077334:Zolpidem; D009584:Nitrogen