Cell cycle-dependent accumulation of a kinesin-like protein, KatB/C in synchronized tobacco BY-2 cells.
Immunoblot analysis with antibodies prepared against highly purified recombinant truncated kinesin-like proteins, KatB(5-249) and KatC(207-754), encoded by the katB and katC genes of Arabidopsis thaliana revealed the presence of a kinesin-like polypeptide, termed KatB/C, in cultured tobacco BY-2 cells. The KatB/C polypeptide cosedimented with microtubules in the presence of a nonhydrolyzable ATP analogue and was released from microtubules in the presence of ATP, both of which are characteristics of kinesin proteins. The amount of KatB/C polypeptide in synchronous BY-2 cells increased during M phase of the cell cycle. Microtubule-based structures present in cells at M phase, such as the spindle and phragmoplast, may be the site of action of the KatB/C protein.
Mitsui
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H
Institute of Molecular and Cellular Biosciences, University of Tokyo, Japan.
Hasezawa
S
S
Nagata
T
T
Takahashi
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H
D017360:Arabidopsis / Q000276:immunology; D029681:Arabidopsis Proteins; D002453:Cell Cycle / Q000502:physiology*; D002478:Cells, Cultured; D003429:Cross Reactions; D006801:Humans; D015151:Immunoblotting; D016547:Kinesins / Q000276:immunology / Q000302:isolation & purification*; D010446:Peptide Fragments / Q000276:immunology; D010940:Plant Proteins / Q000276:immunology / Q000302:isolation & purification*; D010947:Plants, Toxic; D013045:Species Specificity; D014026:Nicotiana / Q000235:genetics*
D029681:Arabidopsis Proteins; D010446:Peptide Fragments; D010940:Plant Proteins; C490295:KATB protein, Nicotiana tabacum; C089152:KatB protein, Arabidopsis; C089153:KatC protein, Arabidopsis; D016547:Kinesins