Ovalbumin-liposome conjugate induces IgG but not IgE antibody production.
Antibody response after immunization with surface-coupled ovalbumin (OVA) of liposomes was investigated in mice. OVA was coupled to the surface of liposome via amino groups using glutaraldehyde. OVA-liposome conjugate induced a significant anti-OVA IgG antibody production in mice. However, no IgE antibody production specific for OVA was observed. Immunization with OVA-liposome induced IgE-specific unresponsiveness even after the subsequent challenge with OVA adsorbed with with aluminium hydroxide (OVA-alum), which induces a high level of IgE antibody production. Furthermore, following the primary immunization with OVA-alum, a secondary challenge with OVA-liposome boosted anti-OVA IgG but not anti-OVA IgE antibody production. These results show the potential of the antigen-liposome conjugate for the development of a vaccine with the least allergic reaction and also for the application of immunotherapy.
Naito
S
S
Department of Safety Research on Biologics, National Institute of Health, Tokyo, Japan.
Horino
A
A
Nakayama
M
M
Nakano
Y
Y
Nagai
T
T
Mizuguchi
J
J
Komuro
K
K
Uchida
T
T
D000536:Aluminum Hydroxide / Q000008:administration & dosage; D000818:Animals; D005260:Female; D007117:Immunization, Secondary; D018796:Immunoconjugates / Q000008:administration & dosage / Q000494:pharmacology*; D007073:Immunoglobulin E / Q000096:biosynthesis*; D007074:Immunoglobulin G / Q000096:biosynthesis*; D008081:Liposomes / Q000008:administration & dosage / Q000276:immunology* / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000008:administration & dosage / Q000276:immunology* / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley
D018796:Immunoconjugates; D007074:Immunoglobulin G; D008081:Liposomes; D007073:Immunoglobulin E; D000536:Aluminum Hydroxide; D010047:Ovalbumin