Signal transduction mechanisms subserving activity-dependent release of neuronal proteoglycans.
We demonstrate here using dissociated hippocampal neurons that glutamate-induced release of proteoglycans which have been shown to have neurite growth-promoting activity is regulated by serine/threonine kinases of the protein kinase C and calcium/calmodulin type II kinase families, and that the state of phosphorylation of hippocampal neurons is a determinant of the magnitude and duration of the release response. Nitric oxide is also involved in mediating glutamate-induced PG release.
Dow
K E
KE
Department of Pediatrics, Queen's University, Kingston, Ont., Canada.
Sugiura
M
M
D000704:Analysis of Variance; D000818:Animals; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000502:physiology; D006624:Hippocampus / Q000166:cytology / Q000196:embryology / Q000378:metabolism*; D009414:Nerve Growth Factors / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism*; D009569:Nitric Oxide / Q000502:physiology; D011493:Protein Kinase C / Q000502:physiology; D011509:Proteoglycans / Q000378:metabolism*; D051381:Rats; D015398:Signal Transduction / Q000502:physiology*
D009414:Nerve Growth Factors; D011509:Proteoglycans; D009569:Nitric Oxide; D011493:Protein Kinase C; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2; D017871:Calcium-Calmodulin-Dependent Protein Kinases