Biliary glycoprotein (BGP) expression on T cells and on a natural-killer-cell sub-population.
Human T and natural-killer (NK) cells, that are thought to be the major cytotoxic effector-cell populations in the defence against neoplastic cells, were isolated from blood and decidua in order to analyze their expression of carcinoembronic-antigen-(CEA)-family-member proteins. Biliary glycoprotein (BGP,CD66a) was the only member of the carcinoembryonic antigen family detected. While freshly isolated T-cells expressed low amounts of BGP, freshly isolated NK cells were negative. After in vitro stimulation for 3 days, T cells up-regulated their BGP expression and a sub-group of NK cells (CD16- CD56+), known to predominate in decidua revealed de novo expression of BGP. In contrast, stimulated CD16+ CD56+ NK cells, which occur exclusively in the blood, remained negative. The expression of BGP could be shown on the protein level by using a panel of 12 well-defined MAbs and on the transcription level in rt-PCR and subsequent oligonucleotide hybridization. Interestingly, rIL-2-stimulated T cells expressed 3-fold higher levels of BGP compared with those seen after stimulation with phytohemagglutinin (PHA). PHA, on the other hand, induced a higher expression of HLA-DR, an activation marker of T cells. The differential regulation implies a distinct function of BGP and HLA-DR.
Moller
M J
MJ
Institute of Immunobiology, Medical Faculty, University of Freiburg, Germany.
Kammerer
R
R
Grunert
F
F
von Kleist
S
S
D015703:Antigens, CD / Q000096:biosynthesis / Q000097:blood; D000943:Antigens, Differentiation / Q000096:biosynthesis / Q000097:blood; D001483:Base Sequence; D019002:CD56 Antigen / Q000096:biosynthesis / Q000097:blood; D002272:Carcinoembryonic Antigen / Q000096:biosynthesis / Q000235:genetics; D015815:Cell Adhesion Molecules; D003656:Decidua / Q000378:metabolism; D000939:Epitopes; D005260:Female; D006023:Glycoproteins / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology; D007694:Killer Cells, Natural / Q000378:metabolism*; D008213:Lymphocyte Activation / Q000187:drug effects; D008969:Molecular Sequence Data; D010835:Phytohemagglutinins / Q000494:pharmacology; D017452:Receptors, IgG / Q000032:analysis / Q000096:biosynthesis; D013268:Stimulation, Chemical; D016176:T-Lymphocyte Subsets / Q000502:physiology*; D013601:T-Lymphocytes / Q000378:metabolism*
D015703:Antigens, CD; D000943:Antigens, Differentiation; D019002:CD56 Antigen; C069847:CD66 antigens; D002272:Carcinoembryonic Antigen; D015815:Cell Adhesion Molecules; D000939:Epitopes; D006023:Glycoproteins; D007376:Interleukin-2; D010835:Phytohemagglutinins; D017452:Receptors, IgG