Cl/HCO(-3) exchanger is operative in isolated enterocytes from rat jejunum.
Enterocytes isolated from rat jejunum were tested for the existence of a Cl-/HCO(-3) exchange, previously evidenced in basolateral membrane vesicles but not in brush border. Cells were found to retain functional integrity and transport capabilities long enough to allow Cl- fluxes to be measured. Both efflux and uptake experiments indicate that a Cl-/HCO(-3) antiport, inhibited by 4,4'-diisothiocyanostilbene-2-2'-disulfonic acid (DIDS), is functional under resting conditions.
Tosco
M
M
Dipartimento di Fisiologia e Biochimica Generali, Università di Milano, Italy.
Orsenigo
M N
MN
Faelli
A
A
D000818:Animals; D017920:Antiporters / Q000378:metabolism*; D001639:Bicarbonates / Q000378:metabolism; D001692:Biological Transport; D002470:Cell Survival; D002478:Cells, Cultured; D027963:Chloride-Bicarbonate Antiporters; D002712:Chlorides / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007583:Jejunum / Q000166:cytology / Q000378:metabolism*; D008297:Male; D051381:Rats; D017208:Rats, Wistar
D017920:Antiporters; D001639:Bicarbonates; D027963:Chloride-Bicarbonate Antiporters; D002712:Chlorides