Rac-dependent and -independent pathways mediate growth factor-induced Ca2+ influx.
We report that expressing interfering mutants of the small Ras-related GTPase Rac, using either recombinant vaccinia virus or stable DNA transfection, eliminates epidermal growth factor-induced Ca2+ signaling, without affecting Ca2+ mobilization or influx from G protein-coupled receptors. Platelet-derived growth factor-dependent Ca2+ influx, however, is only partly sensitive to dominant negative Rac proteins. Thus, whereas epidermal growth factor-induced Ca2+ influx is completely mediated by Rac proteins, platelet-derived growth factor-induced Ca2+ influx involves Rac-dependent and -independent signaling pathways.
Peppelenbosch
M P
MP
Laboratory for Physiological Chemistry, Utrecht University, Universiteitsweg 100, NL-3584 CG Utrecht, The Netherlands.
Tertoolen
L G
LG
de Vries-Smits
A M
AM
Qiu
R G
RG
M'Rabet
L
L
Symons
M H
MH
de Laat
S W
SW
Bos
J L
JL
D000818:Animals; D002118:Calcium / Q000502:physiology*; D002478:Cells, Cultured; D004815:Epidermal Growth Factor / Q000502:physiology; D066246:ErbB Receptors / Q000502:physiology*; D019204:GTP-Binding Proteins / Q000502:physiology*; D006367:HeLa Cells; D006632:Histamine / Q000494:pharmacology; D006801:Humans; D010982:Platelet-Derived Growth Factor / Q000502:physiology; D051381:Rats; D017479:Receptors, Platelet-Derived Growth Factor / Q000502:physiology*; D011994:Recombinant Proteins; D015398:Signal Transduction; D014616:Vaccinia virus; D020744:rac GTP-Binding Proteins; D020741:rho GTP-Binding Proteins
D010982:Platelet-Derived Growth Factor; D011994:Recombinant Proteins; D004815:Epidermal Growth Factor; D006632:Histamine; D066246:ErbB Receptors; D017479:Receptors, Platelet-Derived Growth Factor; D019204:GTP-Binding Proteins; D020744:rac GTP-Binding Proteins; D020741:rho GTP-Binding Proteins; D002118:Calcium