Amino-terminal deletions in the decorin core protein leads to the biosynthesis of proteoglycans with shorter glycosaminoglycan chains.
Analysis of the N-terminal sequence of decorin purified from connective tissues and comparison with the sequence deduced from the cDNA indicate that the nascent proteoglycan has a 14 amino acid residue N-terminal propeptide. Mammalian expression vectors encoding wild-type decorin and decorin with deletions in the propeptide were used to transform COS and CHO cells. Cells transformed with vectors encoding deletion variants of decorin synthesize proteoglycans with shorter galactosaminoglycan chains than cells transformed with wild-type decorin. This effect on the polysaccharide chain length may be due to a lower affinity between the core protein and the glycosyltransferases synthesizing the linkage region. Alternatively, the deletions may affect the intracellular transport of decorin. An antiserum prepared against the N-terminal propeptide immunoprecipitated decorin secreted by cultured cells, showing that decorin is exported with the N-terminal region intact.
Oldberg A
Department of Cell and Molecular Biology, Lund University, Sweden.
Antonsson
P
P
Moses
J
J
Fransson
L A
LA
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D016466:CHO Cells; D002478:Cells, Cultured; D006224:Cricetinae; D058575:Decorin; D016326:Extracellular Matrix Proteins; D005347:Fibroblasts; D006025:Glycosaminoglycans / Q000096:biosynthesis / Q000737:chemistry*; D000882:Haplorhini; D007668:Kidney / Q000166:cytology; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000276:immunology; D014176:Protein Biosynthesis; D011487:Protein Conformation; D011509:Proteoglycans / Q000096:biosynthesis* / Q000737:chemistry* / Q000378:metabolism; D012333:RNA, Messenger; D011817:Rabbits; D051381:Rats; D017384:Sequence Deletion; D014162:Transfection
D058575:Decorin; D016326:Extracellular Matrix Proteins; D006025:Glycosaminoglycans; D010446:Peptide Fragments; D011509:Proteoglycans; D012333:RNA, Messenger