Topology of LcnD, a protein implicated in the transport of bacteriocins from Lactococcus lactis.
Four in-frame translational fusions to both the reporter proteins beta-galactosidase and alkaline phosphatase support a topological model of LcnD, a protein implicated in the transport of several bacteriocins from Lactococcus lactis, in which the N-terminal part is located intracellularly and one transmembrane helix spans the cytoplasmic membrane.
Franke
C M
CM
Department of Genetics, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Kerklaan, Haren, The Netherlands.
Leenhouts
K J
KJ
Haandrikman
A J
AJ
Kok
J
J
Venema
G
G
Venema
K
K
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000648:ultrastructure; D001430:Bacteriocins / Q000378:metabolism; D001692:Biological Transport; D002352:Carrier Proteins / Q000737:chemistry* / Q000235:genetics / Q000648:ultrastructure; D002451:Cell Compartmentation; D004926:Escherichia coli / Q000235:genetics / Q000648:ultrastructure; D017930:Genes, Reporter; D013294:Lactococcus lactis / Q000737:chemistry* / Q000235:genetics; D008565:Membrane Proteins / Q000737:chemistry* / Q000235:genetics / Q000648:ultrastructure; D008969:Molecular Sequence Data; D011487:Protein Conformation; D016364:Reading Frames; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000648:ultrastructure; D017386:Sequence Homology, Amino Acid
D001426:Bacterial Proteins; D001430:Bacteriocins; D002352:Carrier Proteins; C098554:LcnD protein, Lactococcus lactis; D008565:Membrane Proteins; D011993:Recombinant Fusion Proteins