The cytoplasmic and transmembrane domains of AE2 both contribute to regulation of anion exchange by pH.
We have compared regulation by pH of AE1 (band 3)- and AE2-mediated 36Cl- uptake into Xenopus oocytes. 36Cl- influx was assayed at varying extracellular pH (pHo) values between 9.0 and 5.0 under conditions in which corresponding intracellular pH (pHi) values were at or near steady-state. Wild type (WT) AE1 displayed a broad convex pH versus activity curve, with peak activity at pHo 7.0 and 63% of maximal activity at pHo 5.0. In contrast, WT AE2 displayed a steep pH versus activity curve, with peak activity at pHo9.0 and full suppression at pHo 5.0. The structural basis of these differing pH sensitivities was examined by expression of cRNAs encoding chimeric and truncated proteins. Mutant polypeptides were expressed in oocytes and detected at the cell surface. The AE2cyto/AE1memb polypeptide displayed a broad pH versus activity curve similar to that of WT AE1. In contrast, the AE1cyto/AE2memb polypeptide displayed a steep pH versus activity curve, which was shifted toward acid pH values from that of WT AE2 by 0.69 +/- 0.04 pHo units. Moreover, whereas the pH versus activity curves of AE2 Delta99 and WT AE2 were indistinguishable, AE2 Delta510 exhibited a pH versus activity curve acid-shifted from that of WT AE2 by 0.66 +/- 0.13 pHo units (indistinguishable from that of AE1cyto/AE2memb). The data suggest that a pH sensor resides within the transmembrane region of AE2. The affinity for protons of this pH sensor is influenced by a modifier site located between residues 99 and 510 of the N-terminal cytoplasmic domain of AE2. Acidification of oocytes with acetate suggested that pHi accounted for some but not all of the measured pH dependence of AE2.
Zhang
Y
Y
Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02215, USA.
Chernova
M N
MN
Stuart-Tilley
A K
AK
Jiang
L
L
Alper
S L
SL
D000085:Acetates / Q000378:metabolism; D000818:Animals; D027321:Anion Transport Proteins; D017920:Antiporters; D001483:Base Sequence; D001692:Biological Transport; D002712:Chlorides / Q000378:metabolism*; D017931:DNA Primers; D018076:DNA, Complementary; D005260:Female; D006706:Homeostasis; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008565:Membrane Proteins / Q000096:biosynthesis / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D016296:Mutagenesis; D009865:Oocytes / Q000378:metabolism*; D018075:RNA, Complementary; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000378:metabolism; D064606:SLC4A Proteins; D017384:Sequence Deletion; D013997:Time Factors; D014982:Xenopus laevis
D000085:Acetates; D027321:Anion Transport Proteins; D017920:Antiporters; D002712:Chlorides; D017931:DNA Primers; D018076:DNA, Complementary; D008565:Membrane Proteins; D018075:RNA, Complementary; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; D064606:SLC4A Proteins