Monoclonal antibodies recognized bovine leukocyte antigens used in immuno-scanning electron microscopy.
Antibovine leukocyte monoclonal antibodies were tested in order to use them for scanning electron microscopy in backscattered electron imaging mode. The tests revealed that numerous antibovine leukocyte monoclonal antibodies still recognize lightly glutaraldehyde prefixed antigens and can be used to identify various blood cell types. The results of these tests are presented and discussed. Clearcut differences in the surface morphology exist among bovine peripheral blood normal lymphocytes. Expression of IgM and IgG molecules was observed first of all on the surface of B lymphocyte microvilli.
Levkut
M
M
Department of Pathological Anatomy, University of Veterinary Medicine, Kosice, Slovac Republic.
Ponti
W
W
Levkutova
M
M
Quirici
N
N
D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D000954:Antigens, Surface / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology / Q000648:ultrastructure; D002417:Cattle / Q000276:immunology*; D005260:Female; D007962:Leukocytes / Q000276:immunology*; D051379:Mice; D008855:Microscopy, Electron, Scanning; D016253:Microscopy, Immunoelectron; D008871:Microvilli / Q000276:immunology
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface