Comparative study of the sugar chains of alkaline phosphatases purified from rat liver and rat AH-130 hepatoma cells. Occurrence of fucosylated high-mannose-type and hybrid-type sugar chains.
The N-linked sugar chains of alkaline phosphatases, purified from rat AH-130 hepatoma and from normal rat liver, were released quantitatively as oligosaccharides by hydrazinolysis and were labeled by reduction with NaB3H4. A comparative study of their structures revealed that following structural differences are induced by hepatocyte carcinogenesis: complex-type tetraantennary sugar chains and hybrid-type sugar chains appear; outer-chain moieties of the sugar chains of the hepatoma enzyme contain exclusively the Gal(Beta 1-4)GlcNAc groups (type 2 chains) but those of the normal enzyme contain other Gal(Beta 1-)GlcNAc groups and type 2 chains; and novel fucosylated high-mannose-type sugar chains are found in the oligosaccharides of the hepatoma enzyme.
Endo
T
T
Department of Biochemistry, Institute of Medical Science, University of Tokyo, Japan.
Fujiwara
T
T
Ikehara
Y
Y
Kobata
A
A
D000469:Alkaline Phosphatase / Q000737:chemistry*; D000818:Animals; D002240:Carbohydrate Sequence; D006023:Glycoproteins / Q000737:chemistry*; D008099:Liver / Q000201:enzymology*; D008114:Liver Neoplasms, Experimental / Q000201:enzymology*; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry; D010084:Oxidation-Reduction; D010504:Periodic Acid / Q000737:chemistry; D051381:Rats
D006023:Glycoproteins; D009844:Oligosaccharides; D010504:Periodic Acid; D000469:Alkaline Phosphatase