The association of antibodies against human T cell lymphotropic virus type I (HTLV-I) pX gene mutant products with HTLV-I-associated myelopathy/tropical spastic paraparesis.
Antibodies specific for the products of the human T cell lymphotropic virus type I (HTLV-I) pX frame-shift mutants were studied by ELISA. The serum IgG antibodies to the synthetic peptide corresponding to one nucleotide insertion at position 7784 were significantly more common in patients with HTLV-I-associated myelopathy/tropical spastic paraparesis (HAM/TSP) patients than in HTLV-I carriers who had neither HAM/TSP nor adult T cell leukemia (39% vs. 5%). The seropositivities to the other synthetic peptides, which corresponded to the one nucleotide deletion at position 7475 and the internal deletion of nt 7754-7819 and nt 7853-7936, were rare. A genetic study confirmed the presence of the responsible mutation of the pX gene in peripheral blood mononuclear cells and central nervous system tissue from HTLV-I-infected subjects with and without HAM/TSP. These results suggest that HTLV-I pX frame-shift mutants are expressed in vivo in HTLV-I carriers; they also induce antibodies. especially in those with HAM/TSP.
Suzuki
J
J
Department of Neurology, Kyushu University, Fukuoka, Japan.
Kira
J
J
Baba
E
E
Nakamura
M
M
Koyanagi
Y
Y
Nakamura
T
T
Kawano
Y
Y
Yamasaki
K
K
Shirabe
S
S
Hatano
N
N
Hayashi
K
K
Yamamoto
N
N
Kobayashi
T
T
D000328:Adult; D000595:Amino Acid Sequence; D001483:Base Sequence; D002353:Carrier State / Q000821:virology; D004252:DNA Mutational Analysis; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D016368:Frameshift Mutation / Q000235:genetics*; D016370:Gene Products, rex / Q000276:immunology; D016356:Gene Products, tax / Q000235:genetics / Q000276:immunology; D005814:Genes, Viral / Q000235:genetics; D015481:HTLV-I Antibodies / Q000097:blood / Q000134:cerebrospinal fluid; D015368:Human T-lymphotropic virus 1 / Q000235:genetics / Q000276:immunology; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000821:virology; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D015493:Paraparesis, Tropical Spastic / Q000276:immunology* / Q000821:virology; D010455:Peptides / Q000138:chemical synthesis; D015682:Retroviridae Proteins, Oncogenic / Q000235:genetics* / Q000276:immunology; D014157:Transcription Factors; D054334:Viral Regulatory and Accessory Proteins; D015678:Viral Structural Proteins / Q000235:genetics
D016370:Gene Products, rex; D016356:Gene Products, tax; D015481:HTLV-I Antibodies; D010455:Peptides; D015682:Retroviridae Proteins, Oncogenic; D014157:Transcription Factors; D054334:Viral Regulatory and Accessory Proteins; D015678:Viral Structural Proteins; C072343:pX protein, Human T-lymphotropic virus 1