title: Protein tyrosine kinase activity during egg activation is important for morphogenesis at gastrulation in the sea urchin embryo.
abstract: Fertilization triggers a series of preprogrammed events functioning to activate egg metabolism, incorporate the paternal genome, and initiate development. The activity of protein tyrosine kinases during egg activation is required for several steps leading to the first cell division. We now present evidence for an additional protein tyrosine kinase-mediated event that occurs between 30 and 45 min after insemination and is not required until gastrulation, which occurs over 24 hr later. Eggs treated with protein tyrosine kinase inhibitors within this window of time cleaved and formed normal blastulae but could not gastrulate or undergo further development to the pluteus stage. These findings provide the first evidence that some of the control mechanisms used in later development are established during a brief period of time in the fertilized egg and require the action of one or more protein tyrosine kinases.
authors.affiliation: Department of Anatomy and Cell Biology, University of Kansas Medical Center, Kansas City 66160, USA.
authors.forename: W H
authors.initials: WH
authors.lastname: Kinsey
mesh_terms: D000818:Animals; D004789:Enzyme Activation; D005775:Gastrula / Q000502:physiology*; D009024:Morphogenesis; D010063:Ovum / Q000502:physiology*; D011505:Protein-Tyrosine Kinases / Q000378:metabolism*; D012617:Sea Urchins / Q000196:embryology*
chemical_list: D011505:Protein-Tyrosine Kinases