title: Halothane, but not alpha-chloralose, blocks potassium-evoked cortical spreading depression in cats.
abstract: The effects of two anesthetics, halothane and alpha-chloralose, on induction of spreading depression and on extracellular glutamate elevation after intracortical potassium administration were investigated in artificially ventilated (30% oxygen/70% nitrous oxide) cats. High potassium concentrations were achieved using either direct KCl injections (7 microliters, 150 mM via a micropipette) or microdialysis by supplementing 100, 300 or 500 mM KCl, respectively, for 10 min to the perfusion solution (Ringer's). Changes of the cortical DC potential were recorded adjacent (1-2 mm: electrode DC1) and distant (6-7 mm: electrode DC2) to the injection site. Either under halothane (0.75% in the respiratory gas mixture) or under alpha-chloralose (60 mg/kg i.v.) anesthesia, prolonged negative shifts of the DC potential reflecting the elevated potassium levels after KCl injection were measured near the injection site (electrode DC1). In contrast, spreading depressions (transient short DC deflections) were almost exclusively observed under alpha-chloralose. Spreading depressions recorded with electrode DC1 were superimposed on the prolonged negative DC shifts and they propagated frequently to the more distant site (DC2). Upon KCl administration, dose dependent elevations of extracellular glutamate were measured. These elevations were not significantly altered by the type of anesthesia. Our results suggest that in cats, spreading depression induction is affected by anesthesia, i.e., spreading depression induction is inhibited by halothane as compared to alpha-chloralose. Furthermore, factors other than glutamate or high potassium seem to contribute to spreading depression induction.
authors.affiliation: Max-Planck-Institut für neurologische Forschung, Köln (Lindenthal), Germany.
authors.forename: R
authors.initials: R
authors.lastname: Saito
authors.forename: R
authors.initials: R
authors.lastname: Graf
authors.forename: K
authors.initials: K
authors.lastname: Hübel
authors.forename: J
authors.initials: J
authors.lastname: Taguchi
authors.forename: G
authors.initials: G
authors.lastname: Rosner
authors.forename: T
authors.initials: T
authors.lastname: Fujita
authors.forename: W D
authors.initials: WD
authors.lastname: Heiss
mesh_terms: D000818:Animals; D002415:Cats; D002540:Cerebral Cortex / Q000187:drug effects*; D002698:Chloralose / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005260:Female; D018698:Glutamic Acid / Q000494:pharmacology; D006221:Halothane / Q000494:pharmacology*; D008297:Male; D017551:Microdialysis; D010477:Perfusion; D011188:Potassium / Q000494:pharmacology*
chemical_list: D002698:Chloralose; D018698:Glutamic Acid; D011188:Potassium; D006221:Halothane