title: Identification of ORD, a Drosophila protein essential for sister chromatid cohesion.
abstract: Attachment between the sister chromatids is required for proper chromosome segregation in meiosis and mitosis, but its molecular basis is not understood. Mutations in the Drosophila ord gene result in premature sister chromatid separation in meiosis, indicating that the product of this gene is necessary for sister chromatid cohesion. We isolated the ord gene and found that it encodes a novel 55 kDa protein. Some of the ord mutations exhibit unusual complementation properties, termed negative complementation, in which particular alleles poison the activity of another allele. Negative complementation predicts that protein-protein interactions are critical for ORD function. The position and nature of these unusual ord mutations demonstrate that the C-terminal half of ORD is essential for sister chromatid cohesion and suggest that it mediates protein binding.
authors.affiliation: Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, Cambridge, 02142, USA.
authors.forename: S E
authors.initials: SE
authors.lastname: Bickel
authors.forename: D W
authors.initials: DW
authors.lastname: Wyman
authors.forename: W Y
authors.initials: WY
authors.lastname: Miyazaki
authors.forename: D P
authors.initials: DP
authors.lastname: Moore
authors.forename: T L
authors.initials: TL
authors.lastname: Orr-Weaver
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D018797:Cell Cycle Proteins; D002842:Chromatids / Q000502:physiology*; D003001:Cloning, Molecular; D004330:Drosophila / Q000235:genetics*; D029721:Drosophila Proteins; D017344:Genes, Insect; D005816:Genetic Complementation Test; D008297:Male; D008540:Meiosis; D008969:Molecular Sequence Data; D009154:Mutation; D011485:Protein Binding; D011995:Recombination, Genetic; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D013329:Structure-Activity Relationship
chemical_list: D002352:Carrier Proteins; D018797:Cell Cycle Proteins; D029721:Drosophila Proteins; C098924:ORD protein, Drosophila