title: HLA-DQ8 transgenic mice lacking endogenous class II molecules respond to house dust allergens: identification of antigenic epitopes.
abstract: We have introduced HLA-DQ8 (HLADQB*0302 and HLA-DQA*0301) genes into A beta 0 knockout mice. Transgenic animals were immunized with a whole body extract of Dermatophagoides pteronyssinus (Der p), one of the causative agents of house dust mite allergy. Transgenic mice expressing HLA-DQ8 genes elicited HLA-DQ8-restricted responses driven by CD4+ T cells. Synthetic-overlapping peptides representing a major allergen of house dust mite (Der p 2) were synthesized and used as immunogens. HLA-DQ8+ mice responded to three peptides: 8 (residues 61-80), 11 (residues 91-110), and 13 (residues 111-129). The mice produced IL-2, IL-4, and IL-6 response to Der p challenge, suggesting a mixed Th1/Th2 response. These mice represent a new model for studies of the immune basis of allergy.
authors.affiliation: Department of Pediatric Allergy and Immunology, Mayo Clinic, Rochester, MN 55905, USA.
authors.forename: T
authors.initials: T
authors.lastname: Neeno
authors.forename: C J
authors.initials: CJ
authors.lastname: Krco
authors.forename: J
authors.initials: J
authors.lastname: Harders
authors.forename: J
authors.initials: J
authors.lastname: Baisch
authors.forename: S
authors.initials: S
authors.lastname: Cheng
authors.forename: C S
authors.initials: CS
authors.lastname: David
mesh_terms: D000485:Allergens / Q000276:immunology*; D000595:Amino Acid Sequence; D000818:Animals; D039741:Antigens, Dermatophagoides; D002478:Cells, Cultured; D004306:Dose-Response Relationship, Immunologic; D006023:Glycoproteins / Q000276:immunology*; D006683:HLA-DQ Antigens / Q000235:genetics*; D000949:Histocompatibility Antigens Class II / Q000235:genetics* / Q000502:physiology*; D016056:Immunodominant Epitopes / Q000032:analysis*; D008198:Lymph Nodes / Q000166:cytology; D051379:Mice; D008822:Mice, Transgenic / Q000276:immunology*; D008925:Mites / Q000276:immunology*; D008969:Molecular Sequence Data; D018417:Th1 Cells / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology
chemical_list: D000485:Allergens; D039741:Antigens, Dermatophagoides; D006023:Glycoproteins; D006683:HLA-DQ Antigens; D000949:Histocompatibility Antigens Class II; D016056:Immunodominant Epitopes