title: Identification of chromosome 21 materials using the whole chromosome 21 specific library.
abstract: The chromosome in situ suppression hybridization or chromosome painting technic was applied to confirm and eliminate the markers involving chromosome 21 segments using a chromosome 21 DNA library. The library ATCCLL21SNO2 was amplified, directly biotinylated using the polymerase chain reaction. The results demonstrated a translocation of chromosome 21 material on chromosome 2 and X and eliminate the origin of the marker. Thus, the technique provides an important tool to complement the conventional G-banding technic.
authors.affiliation: Department of Pathology, School of Medical Sciences, Universiti Sains Malaysia, Kubang Kerian. Kelantan, Malaysia.
authors.forename: M N
authors.initials: MN
authors.lastname: Isa
authors.forename: E
authors.initials: E
authors.lastname: Boyd
authors.forename: T L
authors.initials: TL
authors.lastname: Turner
authors.forename: J
authors.initials: J
authors.lastname: Tolmie
authors.forename: J M
authors.initials: JM
authors.lastname: Connor
mesh_terms: D000015:Abnormalities, Multiple / Q000235:genetics*; D000328:Adult; D002889:Chromosomes, Human, Pair 2; D002891:Chromosomes, Human, Pair 21; D004314:Down Syndrome / Q000175:diagnosis / Q000235:genetics*; D005260:Female; D015723:Gene Library; D006580:Genetic Carrier Screening; D005819:Genetic Markers; D006801:Humans; D007231:Infant, Newborn; D008607:Intellectual Disability / Q000235:genetics*; D008677:Metaphase; D014178:Translocation, Genetic; D014960:X Chromosome
chemical_list: D005819:Genetic Markers