title: Crystallization and crystal packing of recombinant 3 (or 17) beta-hydroxysteroid dehydrogenase from Comamonas testosteroni ATTC 11996.
abstract: The enzyme 3 (or 17) beta-hydroxysteroid dehydrogenase from Comamonas testosteroni was crystallized. Crystals, of up to 0.6 mm in their longest dimension and suitable for a crystallographic analysis have been obtained by the vapour diffusion method. They belong to the orthorhombic lattice type and diffract to a maximum resolution of 0.23 nm. A final data set obtained by merging data from three crystals resulted in a completeness of 90% with an Rmerge of 6%. A molecular replacement search carried out by using 3 alpha (or 20 beta)-hydroxysteroid dehydrogenase from Streptomyces hydrogenans as a search model allowed us to assign I222 as the correct space group and to propose a model for the crystal packing, with one monomer per asymmetric unit. Thus, the whole unit cell contains two tetramers. The R-factor after rigid body refinement is 48.1%.
authors.affiliation: Karolinska Institutet, NOVUM, Center of Structural Biochemistry, Huddinge, Sweden.
authors.forename: J
authors.initials: J
authors.lastname: Benach
authors.forename: S
authors.initials: S
authors.lastname: Knapp
authors.forename: U C
authors.initials: UC
authors.lastname: Oppermann
authors.forename: O
authors.initials: O
authors.lastname: Hägglund
authors.forename: H
authors.initials: H
authors.lastname: Jörnvall
authors.forename: R
authors.initials: R
authors.lastname: Ladenstein
mesh_terms: D015067:17-Hydroxysteroid Dehydrogenases / Q000737:chemistry*; D015096:3-Hydroxysteroid Dehydrogenases / Q000737:chemistry*; D000595:Amino Acid Sequence; D018360:Crystallography, X-Ray; D006088:Gram-Negative Aerobic Bacteria / Q000201:enzymology* / Q000235:genetics; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000737:chemistry
chemical_list: D011994:Recombinant Proteins; D015067:17-Hydroxysteroid Dehydrogenases; D015096:3-Hydroxysteroid Dehydrogenases