Influence of chemical allograft preservation procedures on the human immunodeficiency virus.
Since chemically preserved allogenic transplants have an established place in reconstructive procedures, the possibility of transferring the human immunodeficiency virus (HIV) with these transplants has been intensively discussed. In this study the authors obtained brain and spleen samples from six HIV-infected cadavers and preserved them with Merthiolate, Cialit, and formaldehyde. After preservation, the tissues were examined for proviral HIV-1 DNA (gag, pol, env) using the polymerase chain reaction. Proviral sequences were clearly demonstrated after the preservation procedure. The results of this study indicate that HIV remains in tissues that have been treated with Merthiolate, formaldehyde, or Cialit. Further investigations are necessary to determine if the virus is in an inactivated or activated form. It can be concluded that, because of the possible transmission of HIV by chemically preserved homografts, serologic screening of donors should be mandatory.
Bujía
J
J
Department of Otorhinolaryngology, Ludwig Maximilians University, Munich, Germany.
Wilmes
E
E
Kastenbauer
E
E
Gürtler
L
L
D001483:Base Sequence; D001769:Blood / Q000821:virology; D001921:Brain / Q000821:virology; D002356:Cartilage / Q000821:virology; D002920:Cialit; D003238:Connective Tissue / Q000821:virology; D005557:Formaldehyde; D006678:HIV / Q000302:isolation & purification*; D015658:HIV Infections / Q000635:transmission*; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D013154:Spleen / Q000821:virology; D013849:Thimerosal; D014021:Tissue Preservation; D014132:Trachea / Q000821:virology; D014184:Transplantation, Homologous
D002920:Cialit; D005557:Formaldehyde; D013849:Thimerosal