Repair of dGMP hydroxyl radical adducts by verbascoside via electron transfer: a pulse radiolysis study.
The repair activity of verbascoside (VER), isolated from Pedicularis spicata, towards the oxidizing hydroxyl radical adduct of dGMP and its reaction mechanism were studied using pulse radiolysis. Upon pulse radiolysis of nitrous oxide saturated aqueous solution of 2'-deoxyguanosine-5'-monophosphate (dGMP) and VER, it was found that the transient absorption spectrum of the hydroxyl adduct of dGMP decays with the formation of that of the phenoxyl radical of VER, several tens of microseconds after the electron pulse. From the formation kinetics of the phenoxyl radical of VER, the rate constant of the repair reaction was determined to be 1.12 x 10(9) dm(3) mol(-1) s(-1).
Li
W
W
Department of Biology, Lanzhou University, China.
Zheng
R
R
Su
B
B
Jia
Z
Z
Li
H
H
Jiang
Y
Y
Yao
S
S
Lin
N
N
D000975:Antioxidants / Q000494:pharmacology*; D004260:DNA Repair; D003848:Deoxyguanine Nucleotides / Q000378:metabolism*; D005960:Glucosides / Q000494:pharmacology*; D017665:Hydroxyl Radical; D010636:Phenols; D011675:Pulse Radiolysis; D059808:Polyphenols
D000975:Antioxidants; D003848:Deoxyguanine Nucleotides; D005960:Glucosides; D017665:Hydroxyl Radical; D010636:Phenols; C058956:acteoside; D059808:Polyphenols; C007257:2'-deoxyguanosine 5'-phosphate