In vitro infection of primary and retrovirus-infected human leukocytes by human foamy virus.
The infectivity of human foamy virus (HFV) was examined in primary and cultured human leukocytes. Cell-free infectious viral stocks of HFV were prepared from the human kidney cell line 293 transfected with an infectious molecular clone of HFV. HFV productively infects a variety of human myeloid and lymphoid cell lines. In addition, primary cell cultures enriched for human CD4+, monocytes and brain-derived microglial cells, were readily infected by HFV. Interestingly, while infected primary CD4+ lymphocytes and microglial cells showed marked cytopathology characteristic of foamy virus, HFV-infected monocyte-derived macrophages failed to show any cytopathology. In addition, marked cytotoxicity due to HFV infection was seen in both human T-cell leukemia virus type 1- and human immunodeficiency virus type 1-infected T-cell lines and in human immunodeficiency virus type 1-infected monocytoid cell lines. Thus, HFV infection produces differential cytopathology in a wide host range of primary human leukocytes and hematopoietic cell lines.
Mikovits
J A
JA
Biological Carcinogenesis Development Program, NCI-Frederick Cancer Research and Development Center, Frederick, Maryland 21702-1201, USA.
Hoffman
P M
PM
Rethwilm
A
A
Ruscetti
F W
FW
D001483:Base Sequence; D002460:Cell Line; D002467:Cell Nucleus / Q000502:physiology / Q000821:virology; D002478:Cells, Cultured; D017931:DNA Primers; D004279:DNA, Viral / Q000032:analysis / Q000096:biosynthesis; D015683:Gene Products, gag / Q000096:biosynthesis; D006801:Humans; D007668:Kidney; D007700:Kinetics; D007962:Leukocytes / Q000821:virology*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011533:Proviruses / Q000502:physiology; D012190:Retroviridae / Q000472:pathogenicity / Q000502:physiology*; D016092:Spumavirus / Q000472:pathogenicity / Q000502:physiology*; D014779:Virus Replication
D017931:DNA Primers; D004279:DNA, Viral; D015683:Gene Products, gag