Enhanced UV sensitivity of yeast cells induced by overexpression of Mg(2+)-dependent protein phosphatase alpha (type 2C alpha).
The UV sensitivity of wild-type Saccharomyces cerevisiae cells was increased 2-fold when rat Mg(2+)-dependent protein phosphatase alpha (protein phosphatase type 2C alpha) was overexpressed in the cells. The overexpression of this enzyme rendered the rad 18 mutant (defective in postreplication repair) more UV-sensitive than was observed in the wild-type cells. However, this increase in UV sensitivity disappeared when the host cells had a rad 1 mutation (defective in excision repair). These results suggest that the Mg(2+)-dependent protein phosphatase overexpressed in the yeast cells inhibited their excision repair system.
Kobayashi
T
T
Department of Biochemistry, Institute of Development, Aging and Cancer, Tohoku University, Sendai, Japan.
Yasui
A
A
Ohnishi
M
M
Kato
S
S
Sasahara
Y
Y
Kusuda
K
K
Chida
N
N
Yanagawa
Y
Y
Hiraga
A
A
Tamura
S
S
D000818:Animals; D004260:DNA Repair; D004261:DNA Replication; D004307:Dose-Response Relationship, Radiation; D015870:Gene Expression; D005838:Genotype; D010749:Phosphoprotein Phosphatases / Q000096:biosynthesis*; D010957:Plasmids; D051381:Rats; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics / Q000528:radiation effects*; D014466:Ultraviolet Rays
D010749:Phosphoprotein Phosphatases