title: Histones and chromatin structure in hyperthermophilic Archaea.
abstract: HMf is a histone from the hyperthermophile Methanothermus fervidus. It is the archetype and most studied member of a family of archaeal histones that have primary sequences and three-dimensional structures in common with the eukaryal nucleosome core histones and that bind and compact DNA molecules into nucleosome-like structures (NLS). HMf preparations are mixtures of two similar, small (approximately 7.5 kDa) polypeptides designated HMfA and HMfB that in vivo form both homodimers and heterodimers. HMfA synthesis predominates during exponential growth but the relative amount of HMfB increases as M. fervidus cells enter the stationary growth phase. Analyses of homogeneous preparations of recombinant (r) (HMfA)2 and (rHMfB)2 have demonstrated that these proteins have different DNA-binding and compaction properties in vitro, consistent with different roles in vivo for the (HMfA)2, (HMfB)2 and HMfA. HmfB dimers, and for the NLS that they form, in regulating gene expression and in genome compaction and stability.
authors.affiliation: Department of Microbiology, Ohio State University, Columbus 43210, USA.
authors.forename: R A
authors.initials: RA
authors.lastname: Grayling
authors.forename: K
authors.initials: K
authors.lastname: Sandman
authors.forename: J N
authors.initials: JN
authors.lastname: Reeve
mesh_terms: D000595:Amino Acid Sequence; D001105:Archaea / Q000254:growth & development / Q000378:metabolism*; D002843:Chromatin / Q000737:chemistry*; D004247:DNA / Q000378:metabolism; D006657:Histones / Q000096:biosynthesis / Q000737:chemistry*; D008969:Molecular Sequence Data; D013329:Structure-Activity Relationship
chemical_list: D002843:Chromatin; D006657:Histones; D004247:DNA