Induction of varicella-zoster virus-neutralizing antibodies in mice by co-infection with recombinant vaccinia viruses expressing the gH or gL gene.
Recombinant vaccinia viruses (VV) expressing the varicella-zoster virus (VZV) glycoprotein H (gH) or glycoprotein L (gL) were constructed. The 94 kDa gH intermediate glycoprotein was synthesized in cell cultures infected with the VV-gH recombinant, but only co-infection with both recombinants resulted in the synthesis of the fully processed 118 kDa gH molecule. The VV-expressed gH and gL formed a complex that displayed the conformational neutralization epitope detectable by means of human VZV gH-specific monoclonal antibody V3. Formation of this epitope was inhibited by tunicamycin but not by monensin. Simultaneous intraperitoneal inoculation of mice with high doses of both VV-gH and VV-gL viruses resulted in the development of VZV-neutralizing, complement-independent antibodies; these antibodies were not detected in mice infected solely with either the VV-gH or the VV-gL recombinant.
Nĕmecková
S
S
Department of Experimental Virology, Institute of Hematology and Blood Transfusion, Praha, Czech Republic. sarkan@earn.cvut.cz
Ludvíková
V
V
Maresová
L
L
Krystofová
J
J
Hainz
P
P
Kutinová
L
L
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis*; D002642:Chick Embryo; D014645:Herpesvirus 3, Human / Q000276:immunology*; D006801:Humans; D008562:Membrane Glycoproteins / Q000276:immunology*; D051379:Mice; D008813:Mice, Inbred ICR; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000276:immunology; D014616:Vaccinia virus / Q000235:genetics; D014764:Viral Proteins / Q000276:immunology*
D000914:Antibodies, Viral; D008562:Membrane Glycoproteins; D011994:Recombinant Proteins; D014764:Viral Proteins; C094648:glycoprotein gL, varicella-zoster virus; C040496:gp 118 protein, Herpesvirus 3, Human