Effect of progesterone and estradiol benzoate on superoxide dismutase activity in the brain of male rats.
The activities of mitochondrial, manganese-containing superoxide dismutase (MnSOD) and cytoplasmic, copper-zinc-containing superoxide dismutase (CuZnSOD) were measured in subcellular fractions of whole brain homogenates prepared from intact and gonadectomized (GDX) male rats, untreated or treated subcutaneously (sc) with a single dose of 2 mg progesterone (P) and/or 5 micrograms estradiol benzoate (EB). Neither MnSOD nor CuZnSOD was affected by the removal of the testes. Similarly, CuZnSOD activity was steady following systemic administration of P and/or EB to intact and GDX animals 2 h or 24 h prior to sacrifice. On the other hand, both P and EB suppressed MnSOD in the brain of either intact or GDX rats. These results suggest involvement of P and EB in the control of MnSOD activity in the brain of male rats.
Pajović
S
S
Laboratory for Molecular Biology and Endocrinology, Vinća Institute for Nuclear Sciences, Belgrade, Yugoslavia.
Saicić
Z S
ZS
Spasić
M B
MB
Petrović
V M
VM
Martinović
J V
JV
D000818:Animals; D001921:Brain / Q000187:drug effects / Q000201:enzymology*; D002474:Cell-Free System; D003593:Cytoplasm / Q000201:enzymology; D004958:Estradiol / Q000494:pharmacology*; D008297:Male; D009919:Orchiectomy; D011374:Progesterone / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism*
D011374:Progesterone; D004958:Estradiol; D013482:Superoxide Dismutase