Specificity of an HLA-DRB1*0401-restricted T cell response to type II collagen.
A panel of HLA-DRB1*0401-restricted CD4+ mouse T cell hybridomas specific for bovine type II collagen were generated from transgenic mice expressing the human HLA-DRA1*0101/-DRB1*0401 and CD4 molecules. The vast majority recognized a single peptide determinant corresponding to residues 261-273 (CII 261-273). This determinant was rapidly defined by the use of a predictive algorithm for peptide binding to DRB1*0401. CII 261-273 is conserved in bovine and human type II collagen and overlaps with an important I-A q - restricted T cell determinant in mice with collagen-induced arthritis. This study demonstrates how HLA-DR and human CD4-transgenic mice can be used to identify a T cell epitope in a potential or candidate autoantigen.
Fugger
L
L
Department of Microbiology and Immunology, Stanford University School of Medicine, USA. fugger@inet.uni-c.dk
Rothbard
J B
JB
Sonderstrup-McDevitt
G
G
D000595:Amino Acid Sequence; D000818:Animals; D001168:Arthritis / Q000276:immunology; D001172:Arthritis, Rheumatoid / Q000276:immunology; D002417:Cattle; D003094:Collagen / Q000737:chemistry / Q000145:classification / Q000276:immunology* / Q000633:toxicity; D003602:Cytotoxicity, Immunologic; D004195:Disease Models, Animal; D000939:Epitopes / Q000737:chemistry / Q000276:immunology*; D006684:HLA-DR Antigens / Q000276:immunology*; D059811:HLA-DRB1 Chains; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007519:Isoantigens / Q000737:chemistry / Q000276:immunology; D051379:Mice; D008805:Mice, Inbred A; D008811:Mice, Inbred DBA; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000276:immunology; D016176:T-Lymphocyte Subsets / Q000276:immunology*
D000939:Epitopes; D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains; C553505:HLA-DRB1*04:01 antigen; D000949:Histocompatibility Antigens Class II; D007519:Isoantigens; D010446:Peptide Fragments; D003094:Collagen