title: Tetanus toxin-sensitive VAMP-related proteins are present in murine macrophages.
abstract: The light chain of tetanus neurotoxin (TeTx) is a zinc endopeptidase specific for VAMP/synaptobrevin (VAMP), a 120-amino-acid integral protein previously described in the small synaptic vesicles of neuronal cells. TeTx has been shown to be active also on nonneuronal cells. By SDS-PAGE and quantitative immunoblotting on proteins derived from murine macrophages (Mphi) exposed to TeTx, we have shown that: (1) VAMP-related proteins are also present in Mphi and (2) such proteins are sensitive to TeTx proteolytic cleavage. The demonstration that TeTx acts on VAMP-related proteins also in Mphi offers a new and useful tool for molecular studies on Mphi exocytosis.
authors.affiliation: Department of Experimental Medicine and Biochemical Sciences, University of Perugia, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Pitzurra
authors.forename: O
authors.initials: O
authors.lastname: Rossetto
authors.forename: A R
authors.initials: AR
authors.lastname: Chimienti
authors.forename: E
authors.initials: E
authors.lastname: Blasi
authors.forename: F
authors.initials: F
authors.lastname: Bistoni
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D017737:Macrophages, Peritoneal / Q000378:metabolism*; D008565:Membrane Proteins / Q000032:analysis* / Q000378:metabolism; D051379:Mice; D050683:R-SNARE Proteins; D013744:Tetanus Toxin / Q000378:metabolism*
chemical_list: D008565:Membrane Proteins; D050683:R-SNARE Proteins; D013744:Tetanus Toxin