title: Defective site-specific integration elements are present in the genome of virulent bacteriophage LL-H of Lactobacillus delbrueckii.
abstract: The phage attachment site, attP, and the integrase-encoding gene, int, are sufficient to promote site-specific integration of the temperate phage mv4 genome into the chromosome of the Lactobacillus delbrueckii host (L. Dupont, B. Boizet-Bonhoure, M. Coddeville, F. Auvray, and P. Ritzenthaler, J. Bacteriol. 177:586--595, 1995). The mv4 genome region containing these elements was compared at the nucleotide and amino acid levels with that of the closely related virulent phage LL-H. Complex DNA rearrangements were identified; a truncated integrase gene and two sites homologous to the mv4 attP site were detected in the genome of the virulent phage LL-H. These observations suggest that the two phages derive from a common temperate ancestor.
authors.affiliation: Department of Biology, University of Oulu, Finland. Merja.Mikkonen@csc.fi
authors.forename: M
authors.initials: M
authors.lastname: Mikkonen
authors.forename: L
authors.initials: L
authors.lastname: Dupont
authors.forename: T
authors.initials: T
authors.lastname: Alatossava
authors.forename: P
authors.initials: P
authors.lastname: Ritzenthaler
mesh_terms: D000595:Amino Acid Sequence; D001435:Bacteriophages / Q000235:genetics*; D001483:Base Sequence; D016679:Genome, Viral; D007778:Lactobacillus / Q000235:genetics* / Q000821:virology; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017421:Sequence Analysis