Expression of RHD and RHCE gene products using retroviral transduction of K562 cells establishes the molecular basis of Rh blood group antigens.
Retroviral-mediated gene transfer using cDNA transcripts of the RHD and RHCE genes resulted in the isolation of K562 clones expressing D and G or c and E antigens, respectively. These results represent the first direct demonstration that the RHD gene encodes the D and G antigens and the RHCE gene encodes the c and E antigens. Both c and E antigens were expressed after transduction of K562 cells with a single cDNA, indicating that the c antigen does not arise by alternative splicing (exon skipping) of the product of the RHCE gene, as has been suggested.
Smythe
J S
JS
International Blood Group Reference Laboratory, Bristol, UK.
Avent
N D
ND
Judson
P A
PA
Parsons
S F
SF
Martin
P G
PG
Anstee
D J
DJ
D000941:Antigens / Q000235:genetics* / Q000276:immunology; D001483:Base Sequence; D001789:Blood Group Antigens / Q000235:genetics* / Q000276:immunology; D003001:Cloning, Molecular; D005434:Flow Cytometry; D018014:Gene Transfer Techniques; D006801:Humans; D008969:Molecular Sequence Data; D014407:Tumor Cells, Cultured
D000941:Antigens; D001789:Blood Group Antigens