title: Human Txk: genomic organization, structure and contiguous physical linkage with the Tec gene.
abstract: Txk is a Tec-family tyrosine kinase expressed in mouse and human T lymphocytes. Among the Tec kinases, Txk is unique in that its amino terminal region does not include a pleckstrin homology domain or other known extended functional region. Txk is encoded at human chromosome 4p12 and at a recognized region of conserved synteny on mouse chromosome 5. The genomic organization of Txk consists of 15 exons with strong exon-intron organizational homology to Btk, the only other Tec-family kinase for which the genomic structure is fully known. The human Tec gene also maps to 4p12 and, based on limited studies reported here, possesses organizational homology with Btk and Txk. We have sequenced a continuous region of DNA that contains 3' Tec and 5' Txk exons separated by only a approximately 1.5 kb intergenic region containing the putative promoter region of Txk. The close physical linkage of these Tec-family tyrosine kinases, which are expressed in different hematopoetic cell lineages, suggests their potential for coordinate cis-regulation.
authors.affiliation: Department of Pediatrics, University of South Florida, St. Petersburg 33701, USA.
authors.forename: Y
authors.initials: Y
authors.lastname: Ohta
authors.forename: R N
authors.initials: RN
authors.lastname: Haire
authors.forename: C T
authors.initials: CT
authors.lastname: Amemiya
authors.forename: R T
authors.initials: RT
authors.lastname: Litman
authors.forename: T
authors.initials: T
authors.lastname: Träger
authors.forename: O
authors.initials: O
authors.lastname: Riess
authors.forename: G W
authors.initials: GW
authors.lastname: Litman
mesh_terms: D000077329:Agammaglobulinaemia Tyrosine Kinase; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002874:Chromosome Mapping; D002894:Chromosomes, Human, Pair 4; D005091:Exons; D015723:Gene Library; D008040:Genetic Linkage; D006412:Hematopoietic Stem Cells / Q000201:enzymology; D006801:Humans; D007438:Introns; D051379:Mice; D008969:Molecular Sequence Data; D011505:Protein-Tyrosine Kinases / Q000235:genetics*; D015183:Restriction Mapping; D013045:Species Specificity
chemical_list: C089848:Tec protein-tyrosine kinase; D011505:Protein-Tyrosine Kinases; D000077329:Agammaglobulinaemia Tyrosine Kinase; C000625949:BTK protein, human