Construction of an alkaline phosphatase fusion-generating transposon, mTn10phoA.
We constructed a derivative of the mini-transposon mTn10 that generates translational fusions to the phoA gene from Escherichia coli and carries the KmR determinant from Tn5. This new transposon, mTn10phoA, is carried on a mobilizable plasmid with both selectable and counterselectable markers. The plasmid carrying mTn10phoA was introduced into Legionella pneumophila. Southern hybridization analysis indicated that the mTn10phoA insertions were randomly distributed.
McClain
M S
MS
Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor 48109-0620, USA. mcclain@umich.edu.
Engleberg
N C
NC
D000469:Alkaline Phosphatase / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods*; D004251:DNA Transposable Elements / Q000235:genetics*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D007613:Kanamycin Resistance / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics*; D012641:Selection, Genetic
D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D011993:Recombinant Fusion Proteins; D000469:Alkaline Phosphatase