SFA-1, a novel cellular gene induced by human T-cell leukemia virus type 1, is a member of the transmembrane 4 superfamily.
A novel cellular gene termed SFA-1 was isolated by differential hybridization of a cDNA library, using probes obtained from an adult T-cell leukemia cell line in comparison with probes obtained from normal CD4+ T cells and the MOLT-4 cell line. The mRNA of the SFA-1 gene is approximately 1.6 kb in size and encodes a protein of 253 amino acids, containing four putative transmembrane domains, a number of cysteine residues, and one potential N-glycosylation site in a major hydrophilic region between the third and fourth transmembrane domains. Expression of the SFA-1 gene was either absent or present at a low level in lymphoid cells but was up-regulated after transformation by human T-cell leukemia virus type 1 and transactivated by Tax. SFA-1 was broadly expressed in many human cell types and conserved in different species. Computer-aided comparison showed that SFA-1 had significant sequence homology and common structural features with members of the transmembrane 4 superfamily. SFA-1 antigen was detected as a 29-kDa membrane protein by immunoblotting, using anti-SFA-1 monoclonal antibody.
Hasegawa
H
H
First Department of Internal Medicine, Ehime University School of Medicine, Shigenobu, Japan.
Utsunomiya
Y
Y
Kishimoto
K
K
Yanagisawa
K
K
Fujita
S
S
D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D015703:Antigens, CD; D001483:Base Sequence; D015496:CD4-Positive T-Lymphocytes / Q000502:physiology; D002460:Cell Line; D015967:Gene Expression Regulation, Viral; D016356:Gene Products, tax / Q000378:metabolism; D015368:Human T-lymphotropic virus 1 / Q000235:genetics*; D006801:Humans; D015458:Leukemia, T-Cell; D008562:Membrane Glycoproteins / Q000096:biosynthesis*; D051379:Mice; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000032:analysis / Q000096:biosynthesis; D017386:Sequence Homology, Amino Acid; D060185:Tetraspanin 24; D014162:Transfection; D014407:Tumor Cells, Cultured
D015703:Antigens, CD; C554715:CD151 protein, human; C554716:Cd151 protein, mouse; D016356:Gene Products, tax; D008562:Membrane Glycoproteins; D011994:Recombinant Proteins; D060185:Tetraspanin 24