The expression of truncated MK in human tumors.
Midkine (MK) is a heparin binding growth/differentiation factor different from fibroblast growth factors (FGFs), and is largely composed of two domains which are found by a folded polypeptide chain interconnected by disulfide bridges. Polymerase chain reaction revealed the presence of a short MK mRNA in 7 among 12 human tumor cells which expressed MK mRNA. All of 4 pancreatic carcinoma cell lines expressed the short species in addition to the full size mRNA. The short mRNA lacked an exon and resulted in the lack of a more N-terminally located domain. The truncated form of MK was found also in some surgically removed specimens of human tumors, but not in noncancerous tissue.
Kaname
T
T
Institute of Molecular Embryology and Genetics, Kumamoto University School of Medicine, Japan. tkan@gpc.kumamoto-u.ac.jp
Kadomatsu
K
K
Aridome
K
K
Yamashita
S
S
Sakamoto
K
K
Ogawa
M
M
Muramatsu
T
T
Yamamura
K
K
D001483:Base Sequence; D015152:Blotting, Northern; D002352:Carrier Proteins / Q000096:biosynthesis*; D002460:Cell Line; D016207:Cytokines / Q000096:biosynthesis; D017931:DNA Primers; D018076:DNA, Complementary; D005091:Exons; D015870:Gene Expression; D006801:Humans; D000078792:Midkine; D008969:Molecular Sequence Data; D009369:Neoplasms / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis*; D012334:RNA, Neoplasm / Q000032:analysis / Q000096:biosynthesis; D012016:Reference Values; D014407:Tumor Cells, Cultured
D002352:Carrier Proteins; D016207:Cytokines; D017931:DNA Primers; D018076:DNA, Complementary; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D000078792:Midkine