Meso-substituted cationic porphyrins as efficient photosensitizers of gram-positive and gram-negative bacteria.
Previous studies on the photosensitization of bacterial cells with different neutral or negatively charged porphyrins and phthalocyanines have demonstrated that, although Gram-positive bacteria are efficiently photoinactivated, Gram-negative bacteria become photosensitive only after modification of the permeability of their outer membrane. The results described in this paper show that two meso-substituted cationic porphyrins, namely tetra(4N-methyl-pyridyl)porphine tetraiodide and tetra(4N,N,N-trimethyl-anilinium)porphine, efficiently photosensitize the inactivation of Gram-negative bacteria, such as Vibrio anguillarum and Escherichia coli. A negatively charged meso-substituted porphyrin, tetra(4-sulphonatophenyl)porphine, has no appreciable photosensitizing activity towards Gram-negative bacteria, although all three porphyrins exhibit a similar subcellular distribution pattern, being mainly localized in the protoplasts or spheroplasts. Moreover, the three porphyrins show similar efficiency in the photoinactivation of the Gram-positive bacterium Entorecoccus seriolicida.
Merchat
M
M
Laboratoire de Recherches L'Oréal, Paris, France.
Bertolini
G
G
Giacomini
P
P
Villanueva
A
A
Jori
G
G
D004307:Dose-Response Relationship, Radiation; D016983:Enterococcus / Q000187:drug effects / Q000528:radiation effects; D004926:Escherichia coli / Q000187:drug effects / Q000528:radiation effects; D006090:Gram-Negative Bacteria / Q000187:drug effects* / Q000528:radiation effects; D006094:Gram-Positive Bacteria / Q000187:drug effects* / Q000528:radiation effects; D007211:Indoles / Q000494:pharmacology*; D054833:Isoindoles; D008027:Light; D017319:Photosensitizing Agents / Q000494:pharmacology*; D011166:Porphyrins / Q000494:pharmacology*; D013329:Structure-Activity Relationship; D014733:Vibrio / Q000187:drug effects / Q000528:radiation effects
D007211:Indoles; D054833:Isoindoles; D017319:Photosensitizing Agents; D011166:Porphyrins; C013647:phthalocyanine