Probe-immobilized affinity chromatography/mass spectrometry.
A new technique has been developed that provides affinity separations directly on the surface of a matrix-assisted laser desorption/ionization mass spectrometer (MALDI-MS) probe. This strategy provides both in situ identification of biomolecules through biospecific antibody/antigen interactions and molecular weight information in a rapid and facile manner. Our technique, which we call probe affinity MS, directly couples the high selectivity of immobilized affinity chromatography with the sensitivity of MALDI-MS. As this technique permits the rapid identification of antigens, ligands, and other compounds from complex biological mixtures, we believe that it will be useful in a wide range of applications in virtually all fields of the life sciences.
Brockman
A H
AH
Complex Carbohydrate Research Center, University of Georgia, Athens 30602-4712, USA.
Orlando
R
R
D000937:Antigen-Antibody Reactions; D001667:Binding, Competitive; D002846:Chromatography, Affinity; D006801:Humans; D007328:Insulin / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D008970:Molecular Weight; D009113:Muramidase / Q000032:analysis* / Q000737:chemistry; D009862:Online Systems; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013666:Tears / Q000737:chemistry / Q000201:enzymology*
D007328:Insulin; D009113:Muramidase