Healing of chondral and osteochondral defects in a canine model: the role of cultured chondrocytes in regeneration of articular cartilage.
In this study a canine model was developed to investigate the nature of early healing responses to both chondral and osteochondral defects and to evaluate the tissue regenerative capacity of cultured autologous chondrocytes in chondral defects. The healing response to surgically created chondral defects was minor, with little cellular infiltration. In contrast, osteochondral defects exhibited a rapid cellular response, resulting ultimately in the formation of fibrous tissue. The lack of significant cellular activity in chondral defects suggests that an evaluation of the capacity of cultured autologous chondrocytes to regenerate articular cartilage is best studied in chondral defects using the canine model. When dedifferentiated cultured articular chondrocytes were implanted into chondral defects, islands of type II collagen staining were demonstrated in the regenerative tissue within 6 weeks. The relatively early expression of cartilage specific markers by the implanted chondrocytes, coupled with the inability of untreated chondral defects to repair or regenerate, demonstrates the utility of the canine model in evaluating novel materials for cartilage repair and regeneration.
Shortkroff
S
S
Brigham & Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.
Barone
L
L
Hsu
H P
HP
Wrenn
C
C
Gagne
T
T
Chi
T
T
Breinan
H
H
Minas
T
T
Sledge
C B
CB
Tubo
R
R
Spector
M
M
D000818:Animals; D001857:Bone Matrix / Q000378:metabolism; D002358:Cartilage, Articular / Q000166:cytology* / Q000502:physiology; D002450:Cell Communication / Q000502:physiology; D002454:Cell Differentiation / Q000502:physiology; D017690:Cell Transplantation / Q000534:rehabilitation*; D002478:Cells, Cultured; D003094:Collagen / Q000378:metabolism; D004195:Disease Models, Animal; D004285:Dogs; D005337:Fibrin / Q000378:metabolism; D007150:Immunohistochemistry; D010007:Osteochondritis / Q000628:therapy*; D012038:Regeneration / Q000502:physiology*
D005337:Fibrin; D003094:Collagen