title: Genetically engineered resistance to dengue-2 virus transmission in mosquitoes.
abstract: The control of arthropod-borne virus diseases such as dengue may ultimately require the genetic manipulation of mosquito vectors to disrupt virus transmission to human populations. To reduce the ability of mosquitoes to transmit dengue viruses, a recombinant Sindbis virus was used to transduce female Aedes aegypti with a 567-base antisense RNA targeted to the premembrane coding region of dengue type 2 (DEN-2) virus. The transduced mosquitoes were unable to support replication of DEN-2 virus in their salivary glands and therefore were not able to transmit the virus.
authors.affiliation: Arthropod-Borne and Infectious Diseases Laboratory (AIDL), Department of Microbiology, Colorado State University, Fort Collins 80523, USA.
authors.forename: K E
authors.initials: KE
authors.lastname: Olson
authors.forename: S
authors.initials: S
authors.lastname: Higgs
authors.forename: P J
authors.initials: PJ
authors.lastname: Gaines
authors.forename: A M
authors.initials: AM
authors.lastname: Powers
authors.forename: B S
authors.initials: BS
authors.lastname: Davis
authors.forename: K I
authors.initials: KI
authors.lastname: Kamrud
authors.forename: J O
authors.initials: JO
authors.lastname: Carlson
authors.forename: C D
authors.initials: CD
authors.lastname: Blair
authors.forename: B J
authors.initials: BJ
authors.lastname: Beaty
mesh_terms: D000330:Aedes / Q000235:genetics / Q000821:virology*; D000818:Animals; D003715:Dengue / Q000635:transmission*; D003716:Dengue Virus / Q000235:genetics* / Q000502:physiology; D004064:Digestive System / Q000821:virology; D005260:Female; D005818:Genetic Engineering; D005822:Genetic Vectors; D016679:Genome, Viral; D006801:Humans; D007303:Insect Vectors / Q000235:genetics / Q000821:virology*; D016372:RNA, Antisense / Q000235:genetics*; D012469:Salivary Glands / Q000821:virology; D012845:Sindbis Virus / Q000235:genetics; D014779:Virus Replication
chemical_list: D016372:RNA, Antisense