The 3-phosphoglycerate kinase gene of the yeast Yarrowia lipolytica de-represses on gluconeogenic substrates.
We have isolated the 3-phosphoglycerate kinase (PGK) gene of the yeast Yarrowia lipolytica by probing a genomic library with a PCR fragment amplified with primers deduced from two highly conserved regions of various PGKs. It is a unique sequence encoding a polypeptide of 417 residues with extensive homology to other PGKs, especially to that of Aspergillus nidulans (76% identity). The expression of the Y. lipolytica PGK1 gene proved to be higher on gluconeogenic substrates than on glycolytic ones. Haploid strains harboring a disrupted allele were able to grow on mixtures of a gluconeogenic carbon source and of a glycolytic one, but required proline supplementation in the presence of glucose, and were inhibited by glycerol.
Le Dall
M
M
Laboratoire de Génétique Moléculaire et Cellulaire, INRA-CNRS, CBAI, Institut National Agronomique, F-78850 Thiverval-Grignon, France.
Nicaud
J
J
Tréton
B Y
BY
Gaillardin
C M
CM
D000595:Amino Acid Sequence; D001483:Base Sequence; D004794:Enzyme Repression; D004926:Escherichia coli / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic; D005800:Genes, Fungal; D005943:Gluconeogenesis; D006019:Glycolysis; D008969:Molecular Sequence Data; D016296:Mutagenesis; D010735:Phosphoglycerate Kinase / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D004718:Saccharomycetales / Q000201:enzymology / Q000235:genetics*; D017422:Sequence Analysis, DNA; D015003:Yeasts / Q000201:enzymology / Q000235:genetics*
D011993:Recombinant Fusion Proteins; D010735:Phosphoglycerate Kinase