title: Glycated Cu,Zn-superoxide dismutase in rat lenses: evidence for the presence of fragmentation in vivo.
abstract: Cu,Zn-superoxide dismutase (Cu,Zn-SOD) exists in tissues of rats as both glycated and non-glycated forms when separated by boronate acid column chromatography. Glycated Cu.Zn-SOD is most abundant in rat lenses compared to other tissues. In normal rats lens levels of glycated Cu.Zn-SOD showed a gradual increase with age, whereas in diabetic rats substantial increases were observed. Immunoblotting analyses, using anti-hexitol lysine IgG, indicated that glycated Cu.Zn-SOD contains Amadori products. Moreover, Cu.Zn-SOD in lenses was site-specifically fragmented probably because of glycation. This the first report of a fragmented protein, such as Cu,Zn-SOD, occurring in vivo.
authors.affiliation: Department of Pediatrics, Osaka Medical College, Japan.
authors.forename: I
authors.initials: I
authors.lastname: Takata
authors.forename: N
authors.initials: N
authors.lastname: Kawamura
authors.forename: T
authors.initials: T
authors.lastname: Myint
authors.forename: N
authors.initials: N
authors.lastname: Miyazawa
authors.forename: K
authors.initials: K
authors.lastname: Suzuki
authors.forename: N
authors.initials: N
authors.lastname: Maruyama
authors.forename: M
authors.initials: M
authors.lastname: Mino
authors.forename: N
authors.initials: N
authors.lastname: Taniguchi
mesh_terms: D000375:Aging / Q000378:metabolism*; D000818:Animals; D002846:Chromatography, Affinity; D003921:Diabetes Mellitus, Experimental / Q000201:enzymology*; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D006031:Glycosylation; D015151:Immunoblotting; D007074:Immunoglobulin G; D007668:Kidney / Q000201:enzymology; D007908:Lens, Crystalline / Q000201:enzymology* / Q000254:growth & development; D008099:Liver / Q000201:enzymology; D008970:Molecular Weight; D009928:Organ Specificity; D010179:Pancreas / Q000201:enzymology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012016:Reference Values; D013482:Superoxide Dismutase / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D006023:Glycoproteins; D007074:Immunoglobulin G; D013482:Superoxide Dismutase