Selectable cassettes for simplified construction of yeast gene disruption vectors.
Cassettes based on a hisG-URA3-hisG insert have been modified by the addition of an KmR-encoding gene and flanking polylinker sites, greatly simplifying construction of gene disruption vectors in Escherichia coli. After gene disruption in yeast, URA3 can then be excised by recombination between the hisG repeats flanking the gene, permitting reuse of the URA3 marker.
Earley
M C
MC
Graduate Program in Genetics and Molecular Biology, Emory University, Atlanta, GA 30322, USA.
Crouse
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GF
D003001:Cloning, Molecular / Q000379:methods; D005822:Genetic Vectors; D007613:Kanamycin Resistance; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional / Q000379:methods*; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics*