Expression of fibronectin and its receptor in some tumour cell lines and in HIV-1-infected cells.
The aim of our study was to evaluate the levels of fibronectin (FN) and its classic receptor (FNR) in various transformed cells lines, especially of leukemic origin, and also the influence of HIV-1 replication on the expression of these proteins (in particular on H9-V cells, chronically infected with HIV-1, and acutely infected MT-4 cells). Monoclonal antibodies were used for indirect immunofluorescence tests; the fluorescein-conjugated recombinant p14, the product of the HIV gene tat, was used as a molecular probe. The results demonstrated a high variability of FN and FNR expression among the various cellular lines studied. Moreover, deficits of such adhesive proteins did not necessarily correlate with a severe reduction of the corresponding receptor. HIV-1 replication in MT-4 and H9-V cells increased the expression of FNR. This seems to correlate with p14-induced phenomena because pretreatment of H9-V cells with recombinant p14 showed an enhancing effect on the expression of this receptor.
Pugliese
A
A
Institute of Infectious Diseases, University of Turin, Italy.
Comito
G
G
Savarino
A
A
Cantamessa
C
C
Torre
D
D
D000650:Amnion / Q000166:cytology; D001402:B-Lymphocytes / Q000166:cytology / Q000378:metabolism / Q000821:virology; D005353:Fibronectins / Q000096:biosynthesis*; D019084:Fluorescent Antibody Technique, Indirect; D015658:HIV Infections / Q000378:metabolism; D015497:HIV-1 / Q000378:metabolism*; D006801:Humans; D007945:Leukemia, Lymphoid; D007951:Leukemia, Myeloid; D008223:Lymphoma; D017469:Receptors, Fibronectin / Q000096:biosynthesis*; D013601:T-Lymphocytes / Q000166:cytology / Q000378:metabolism / Q000821:virology; D014407:Tumor Cells, Cultured / Q000737:chemistry / Q000378:metabolism / Q000821:virology; D001749:Urinary Bladder Neoplasms
D005353:Fibronectins; D017469:Receptors, Fibronectin