title: A morphometric analysis of exocytosis in KCl-stimulated bovine chromaffin cells.
abstract: Transmission electron microscopy has been used to morphometrically evaluate exocytosis in bovine adrenal medulla chromaffin cells as the mechanism of catecholamine release. Purified cell suspensions were stimulated with KCl at varying strengths and durations and then conventionally processed for ultrastructural analysis. Quantitation of exocytotic images of dense cored chromaffin granules was a major objective and such images were found in all preparations, attesting to the efficacy of chemical fixation to preserve this event. However, because hundreds of cell profiles had to be screened to find a single granule in the process of release this low frequency precluded any meaningful correlations with estimates of granular involvement based on catecholamine release. Neither KCl molarity nor duration altered this finding nor did these variables significantly affect other parameters linked to exocytotic activity. For example, cell size and numbers of "empty' granules and vesicles remained constant and attempts to label "any' organelle with 30-nm colloidal gold or lanthanum precipitate proved unsuccessful. In short, if exocytosis is responsible for release, it would appear to function without leaving a morphological trace. An alternative hypothesis, therefore, is outlined which better accommodates existing data.
authors.affiliation: Arbeitsgruppe Elektronenmikroskopie, Max-Planck-Institut für biophysikalische Chemie, Göttingen, Germany.
authors.forename: G Q
authors.initials: GQ
authors.lastname: Fox
mesh_terms: D000313:Adrenal Medulla / Q000166:cytology; D000818:Animals; D002395:Catecholamines / Q000378:metabolism; D002417:Cattle; D002462:Cell Membrane / Q000502:physiology / Q000648:ultrastructure; D048429:Cell Size; D002838:Chromaffin System / Q000166:cytology* / Q000648:ultrastructure; D003594:Cytoplasmic Granules / Q000502:physiology / Q000648:ultrastructure; D005089:Exocytosis / Q000187:drug effects / Q000502:physiology*; D007425:Intracellular Membranes / Q000502:physiology / Q000648:ultrastructure; D008854:Microscopy, Electron; D011189:Potassium Chloride / Q000494:pharmacology*
chemical_list: D002395:Catecholamines; D011189:Potassium Chloride