title: Alterations of lipoprotein fluidity by non-esterified fatty acids known to affect cholesteryl ester transfer protein activity. An electron spin resonance study.
abstract: The aim of the present study was to investigate the effect of saturated, monounsaturated and polyunsaturated non-esterified fatty acids (NEFA) on lipoprotein fluidity by using the electron spin resonance (ESR) method. The fluidity of the lipid phase of lipoproteins was evaluated by calculating from ESR spectra the S parameter of three different positional isomers of spin-labeled stearic acid incorporated into the lipoprotein. In non-enriched lipoproteins, S values were higher in high-density lipoprotein 3 (HDL3) than in low-density lipoprotein (LDL) indicating that the surface of HDL3 was more ordered. Prior incubation of lipoprotein particles with NEFA significantly reduced S values, indicating an increased lipoprotein fluidity as compared with non-supplemented homologous samples. In NEFA-enriched lipoproteins, the modifications in fluidity were shown to be dependent on the structure of the NEFA acyl carbon chains. Medium-chain fatty acids [lauric (12:0) and myristic (14:0) acids] appeared to be better fluidizing molecules as compared with both shorter [octanoic (8:0) and decanoic (10:0) acids] and longer [palmitic (16:0) and stearic (18:0) acids] homologues. In addition, introducing at least one double bond in the acyl carbon chain significantly increased the ability of NEFA to reduce S as compared with saturated homologues. In both LDL and HDL3, the extent of the modifications of the molecular mobility at the lipoprotein surface was dependent on the final NEFA/lipoprotein ratio. In conclusion, these results suggest that the ability of NEFA to modulate the activity of the cholesteryl ester transfer protein might relate in part to alterations in fluidity at the lipoprotein surface.
authors.affiliation: Laboratoire de Biochimie des Lipoprotéines, INSERM CJF 93-10, Facultéde Médecine, Dijon, France.
authors.forename: C
authors.initials: C
authors.lastname: Foucher
authors.forename: L
authors.initials: L
authors.lastname: Lagrost
authors.forename: V
authors.initials: V
authors.lastname: Maupoil
authors.forename: M
authors.initials: M
authors.lastname: le Meste
authors.forename: L
authors.initials: L
authors.lastname: Rochette
authors.forename: P
authors.initials: P
authors.lastname: Gambert
mesh_terms: D002352:Carrier Proteins / Q000737:chemistry*; D053480:Cholesterol Ester Transfer Proteins; D002788:Cholesterol Esters / Q000737:chemistry*; D004578:Electron Spin Resonance Spectroscopy; D005230:Fatty Acids, Nonesterified / Q000737:chemistry; D006023:Glycoproteins; D006801:Humans; D008074:Lipoproteins / Q000737:chemistry*; D008560:Membrane Fluidity; D019814:Myristic Acid; D009227:Myristic Acids / Q000737:chemistry
chemical_list: C506642:CETP protein, human; D002352:Carrier Proteins; D053480:Cholesterol Ester Transfer Proteins; D002788:Cholesterol Esters; D005230:Fatty Acids, Nonesterified; D006023:Glycoproteins; D008074:Lipoproteins; D009227:Myristic Acids; D019814:Myristic Acid