title: c-myc oncogene expression and cell proliferation in mixed oligo-astrocytoma.
abstract: Mixed gliomas (oligo-astrocytomas) are brain tumours with an admixture of 2 different cell populations: astrocytes and oligodendroglia. On the basis of histological features and behaviour, these tumours are classified as low-grade mixed gliomas (MG) and malignant mixed gliomas (MMG). We have studied the relationship between c-myc protein expression and cellular proliferation in this class of tumours. Using antibody c-33 for c-myc and PC-10 for the proliferating cell nuclear antigen (PCNA), immunohistochemistry was performed on 14 MG and 9 MMG. PCNA was increased in MMG as compared to MG in both astrocytic and oligodendroglial areas. However, more c-myc-positive cells were seen only in the astrocyte areas of MMG. Analysis of the relationship of c-myc and PCNA suggests that the correlation of c-myc with cellular proliferation is dependent on tissue type and differentiation status.
authors.affiliation: Department of Pathology, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, India.
authors.forename: M
authors.initials: M
authors.lastname: Banerjee
authors.forename: A K
authors.initials: AK
authors.lastname: Dinda
authors.forename: S
authors.initials: S
authors.lastname: Sinha
authors.forename: C
authors.initials: C
authors.lastname: Sarkar
authors.forename: M
authors.initials: M
authors.lastname: Mathur
mesh_terms: D000911:Antibodies, Monoclonal; D001253:Astrocytes / Q000473:pathology; D001254:Astrocytoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D001932:Brain Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D002455:Cell Division / Q000502:physiology; D015870:Gene Expression; D016259:Genes, myc; D005910:Glioma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D009836:Oligodendroglia / Q000473:pathology; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis*; D016271:Proto-Oncogene Proteins c-myc / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics
chemical_list: D000911:Antibodies, Monoclonal; D018809:Proliferating Cell Nuclear Antigen; D016271:Proto-Oncogene Proteins c-myc