Karyotyping human chromosomes by combinatorial multi-fluor FISH.
We have developed epifluorescence filter sets and computer software for the detection and discrimination of 27 different DNA probes hybridized simultaneously. For karyotype analysis, a pool of human chromosome painting probes, each labelled with a different fluor combination, was hybridized to metaphase chromosomes prepared from normal cells, clinical specimens, and neoplastic cell lines. Both simple and complex chromosomal rearrangements could be detected rapidly and unequivocally; many of the more complex chromosomal abnormalities could not be delineated by conventional cytogenetic banding techniques. Our data suggest that multiplex-fluorescence in situ hybridization (M-FISH) could have wide clinical utility and complement standard cytogenetics, particularly for the characterization of complex karyotypes.
Speicher
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MR
Department of Genetics, Yale University School of Medicine, New Haven, Connecticut 06510, USA.
Gwyn Ballard
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S
Ward
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DC
D002869:Chromosome Aberrations / Q000235:genetics; D018244:Chromosomes, Artificial, Yeast; D002877:Chromosomes, Human / Q000235:genetics* / Q000648:ultrastructure; D015342:DNA Probes; D005260:Female; D005456:Fluorescent Dyes; D015321:Gene Rearrangement; D006801:Humans; D007091:Image Processing, Computer-Assisted; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D007621:Karyotyping / Q000379:methods*; D008297:Male; D012984:Software; D014407:Tumor Cells, Cultured
D015342:DNA Probes; D005456:Fluorescent Dyes