title: GATA transcription factors associate with a novel class of nuclear bodies in erythroblasts and megakaryocytes.
abstract: The nuclear distribution of GATA transcription factors in murine haemopoietic cells was examined by indirect immunofluorescence. Specific bright foci of GATA-1 fluorescence were observed in erythroleukaemia cells and primary murine erythroblasts and megakaryocytes, in addition to diffuse nucleoplasmic localization. These foci, which were preferentially found adjacent to nucleoli or at the nuclear periphery, did not represent sites of active transcription or binding of GATA-1 to consensus sites in the beta-globin loci. Immunoelectron microscopy demonstrated the presence of intensely labelled structures likely to represent the GATA-1 foci seen by immunofluorescence. The GATA-1 nuclear bodies differed from previously described nuclear structures and there was no co-localization with nuclear antigens involved in RNA processing or other ubiquitous (Spl, c-Jun and TBP) or haemopoietic (NF-E2) transcription factors. Interestingly, GATA-2 and GATA-3 proteins also localized to the same nuclear bodies in cell lines co-expressing GATA-1 and -2 or GATA-1 and -3 gene products. This pattern of distribution is, thus far, unique to the GATA transcription factors and suggests a protein-protein interaction with other components of the nuclear bodies via the GATA zinc finger domain.
authors.affiliation: National Institute for Medical Research, UK.
authors.forename: A G
authors.initials: AG
authors.lastname: Elefanty
authors.forename: M
authors.initials: M
authors.lastname: Antoniou
authors.forename: N
authors.initials: N
authors.lastname: Custodio
authors.forename: M
authors.initials: M
authors.lastname: Carmo-Fonseca
authors.forename: F G
authors.initials: FG
authors.lastname: Grosveld
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D001483:Base Sequence; D002467:Cell Nucleus / Q000378:metabolism* / Q000648:ultrastructure; D002478:Cells, Cultured; D004268:DNA-Binding Proteins / Q000032:analysis / Q000378:metabolism; D000939:Epitopes; D004900:Erythroblasts / Q000166:cytology / Q000378:metabolism*; D050981:Erythroid-Specific DNA-Binding Factors; D019084:Fluorescent Antibody Technique, Indirect; D050982:GATA1 Transcription Factor; D050989:GATA2 Transcription Factor; D050990:GATA3 Transcription Factor; D006412:Hematopoietic Stem Cells / Q000378:metabolism*; D006801:Humans; D004915:Leukemia, Erythroblastic, Acute; D008533:Megakaryocytes / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D016253:Microscopy, Immunoelectron; D008969:Molecular Sequence Data; D050983:NF-E2 Transcription Factor; D050984:NF-E2 Transcription Factor, p45 Subunit; D009838:Oligodeoxyribonucleotides; D016271:Proto-Oncogene Proteins c-myc / Q000096:biosynthesis / Q000235:genetics; D015534:Trans-Activators / Q000032:analysis / Q000378:metabolism; D014157:Transcription Factors / Q000032:analysis* / Q000378:metabolism; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
chemical_list: D000911:Antibodies, Monoclonal; D004268:DNA-Binding Proteins; D000939:Epitopes; D050981:Erythroid-Specific DNA-Binding Factors; D050982:GATA1 Transcription Factor; C494688:GATA1 protein, human; D050989:GATA2 Transcription Factor; C494711:GATA2 protein, human; D050990:GATA3 Transcription Factor; C494749:GATA3 protein, human; C494689:Gata1 protein, mouse; C494712:Gata2 protein, mouse; C494748:Gata3 protein, mouse; D050983:NF-E2 Transcription Factor; D050984:NF-E2 Transcription Factor, p45 Subunit; C494692:NFE2 protein, human; C494693:Nfe2 protein, mouse; D009838:Oligodeoxyribonucleotides; D016271:Proto-Oncogene Proteins c-myc; D015534:Trans-Activators; D014157:Transcription Factors