title: Effect of NADPH-associated keto-reducing domain on substrate entry into 6-hydroxymellein synthase, a multifunctional polyketide synthetic enzyme involved in phytoalexin biosynthesis in carrot.
abstract: 6-Hydroxymellein synthase is a polyketide biosynthetic enzyme induced in carrot cells which is organized as a homodimer composed of multifunctional subunits. The synthase liberates triacetic acid lactone, instead of 6-hydroxymellein, as a derailment product when the keto-reducing reaction at the triketide intermediate stage is interrupted. However, the efficiency of the triacetic acid lactone-forming reactions is markedly lower than that of the normal reaction, and the kinetic analyses have revealed that the affinity of the enzyme protein for acetyl-CoA is appreciably reduced in the abnormal reactions. It is assumed that the interaction of the NADPH-associated keto-reducing domain with a putative primary binding site(s) of the acyl-CoA in the enzyme structure affects the entry of the starter unit into the protein. The present finding should provide an example of the novel class of "subunit communication" of multimer enzymes.
authors.affiliation: Cell Biology Laboratory, Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University, Japan.
authors.forename: F
authors.initials: F
authors.lastname: Kurosaki
mesh_terms: D000105:Acetyl Coenzyme A / Q000378:metabolism*; D000217:Acyltransferases / Q000378:metabolism*; D018552:Daucus carota / Q000201:enzymology*; D007700:Kinetics; D008025:Ligases / Q000378:metabolism*; D008316:Malonyl Coenzyme A / Q000378:metabolism; D008956:Models, Chemical; D009097:Multienzyme Complexes / Q000378:metabolism*; D009249:NADP / Q000378:metabolism; D010088:Oxidoreductases / Q000378:metabolism*; D010936:Plant Extracts / Q000096:biosynthesis*; D011753:Pyrones / Q000378:metabolism; D012717:Sesquiterpenes; D013729:Terpenes / Q000378:metabolism*; D000094162:Phytoalexins
chemical_list: D009097:Multienzyme Complexes; D010936:Plant Extracts; D011753:Pyrones; D012717:Sesquiterpenes; D013729:Terpenes; D008316:Malonyl Coenzyme A; D009249:NADP; C030720:triacetic acid lactone; D000105:Acetyl Coenzyme A; D010088:Oxidoreductases; D000217:Acyltransferases; C070623:6-hydroxymellein synthetase; D008025:Ligases; D000094162:Phytoalexins