Chromatographic properties of cytosine, cytidine and their synthetic analogues.
A direct reversed-phase high-performance liquid chromatographic (RP-HPLC) assay was used for the study of the effects of methanol concentration, pH, flow-rate of the mobile phase and column temperature on the retention of the natural nuclei acid components cytosine and cytidine and their synthetic 1-beta-D-arabinofuranosyl, 5-aza and 6-aza analogues. The pKa values were also determined. The greatest changes were observed with changes in pH. The relationship between the capacity factors and the hydrophobicity of the compounds studied was also investigated.
Romanová
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Department of Experimental Therapy, Slovak Academy of Sciences, Bratislava, Slovak Republic.
Novotný
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D002851:Chromatography, High Pressure Liquid; D003562:Cytidine / Q000031:analogs & derivatives / Q000032:analysis*; D003596:Cytosine / Q000031:analogs & derivatives / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D000432:Methanol; D013056:Spectrophotometry, Ultraviolet
D003562:Cytidine; D003596:Cytosine; D000432:Methanol