title: Analysis of regulatory elements of the developmentally controlled chorion s15 promoter in transgenic Drosophila.
abstract: The Drosophila s15 chorion gene is expressed only in the follicular epithelium surrounding the developing oocyte, with tight quantitative control and a very narrow temporal specificity. We have used germ-line transformation analysis to conduct an extensive mutational dissection of its promoter between -189 and -39 bp relative to the transcriptional start site. Quantitative control and temporal specificity are disrupted by several of these mutations. The results suggest that this 150-bp DNA region encompasses many positive and negative, at least partially degenerate, cis-regulatory elements, which are involved in specifying the highly precise expression pattern of the s15 gene during development.
authors.affiliation: Department of Biochemistry and Molecular Biology, Thomas Jefferson University, Philadelphia, PA 19107-5099, USA.
authors.forename: B D
authors.initials: BD
authors.lastname: Mariani
authors.forename: M J
authors.initials: MJ
authors.lastname: Shea
authors.forename: M J
authors.initials: MJ
authors.lastname: Conboy
authors.forename: I
authors.initials: I
authors.lastname: Conboy
authors.forename: D L
authors.initials: DL
authors.lastname: King
authors.forename: F C
authors.initials: FC
authors.lastname: Kafatos
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D030801:Animals, Genetically Modified; D001483:Base Sequence; D004331:Drosophila melanogaster / Q000196:embryology / Q000235:genetics*; D004527:Egg Proteins / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D017344:Genes, Insect; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics; D016415:Sequence Alignment; D017384:Sequence Deletion; D017386:Sequence Homology, Amino Acid
chemical_list: D004527:Egg Proteins; D012333:RNA, Messenger