title: Peanut bud necrosis tospovirus S RNA: complete nucleotide sequence, genome organization and homology to other tospoviruses.
abstract: The complete nucleotide sequence of the S RNA of peanut bud necrosis virus (PBNV) has been determined. The RNA is 3 057 nucleotides in length, contains inverted repeats and two open reading frames (ORFs) with an ambisense coding strategy that are separated by an A+U-rich intergenic region. One ORF (1 320 nucleotides in the viral sense strand) encodes a Mr 49.5 kDa protein, identified as the nonstructural (NSs) protein based on similarity to published tospovirus sequences. The second ORF (831 nucleotides in virus complementary strand) encodes a Mr 30.6 kDa protein. This protein was identified as the nucleocapsid (N) protein based on sequence similarities. Amino acid sequence comparison of N and NSs proteins revealed identities of 22-34% with the reported tospovirus isolates of serogroups I, II, and III, whereas it had 82-86% identity with viruses in serogroup IV, watermelon silver mottle virus (WSMV) and tomato isolate of peanut bud necrosis (PBNV-To). Two subgenomic RNA species detected in PBNV infected tissue corresponded to the predicted sizes (1.65 and 1.4 kb) of the NSs and N mRNAs. The data presented show conclusively that PBNV should be included in serogroup IV, along with WSMV and PBNV-To.
authors.affiliation: Crop Protection Division, International Crops Research Institute for the Semi-Arid Tropics Asia Center (ICRISAT-IAC), Patancheru, India.
authors.forename: T
authors.initials: T
authors.lastname: Satyanarayana
authors.forename: S E
authors.initials: SE
authors.lastname: Mitchell
authors.forename: D V
authors.initials: DV
authors.lastname: Reddy
authors.forename: S
authors.initials: S
authors.lastname: Brown
authors.forename: S
authors.initials: S
authors.lastname: Kresovich
authors.forename: R
authors.initials: R
authors.lastname: Jarret
authors.forename: R A
authors.initials: RA
authors.lastname: Naidu
authors.forename: J W
authors.initials: JW
authors.lastname: Demski
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002213:Capsid / Q000737:chemistry / Q000235:genetics; D003001:Cloning, Molecular; D017931:DNA Primers; D018076:DNA, Complementary; D016679:Genome, Viral; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000737:chemistry* / Q000235:genetics* / Q000302:isolation & purification; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D017865:Tospovirus / Q000145:classification / Q000235:genetics*; D014758:Viral Core Proteins / Q000737:chemistry / Q000235:genetics; D017361:Viral Nonstructural Proteins / Q000737:chemistry / Q000235:genetics
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D012367:RNA, Viral; D014758:Viral Core Proteins; D017361:Viral Nonstructural Proteins