Carbon fibre micro-electrode and in vitro or in brain slices voltammetric measurement of ascorbate, catechol and indole oxidation signals: influence of temperature and physiological media.
Carbon fibre micro-electrodes have been used to determine the influence of temperature and physiological media on the oxidation potential value of three carboxylic acids of physiological interest such as ascorbate (AA), 3,4-dihydroxyphenylacetic acid (DOPAC) and 5-hydroxyindolacetic acid (5HIAA). Standard calibrations at room temperature (18-20 degrees C) in phosphate buffered saline (PBS, pH 7.4), in Krebs (pH 7.4) or in artificial cerebral spinal fluid (ACSF, pH 7.4) have been compared with calibrations performed at 37 degrees C under 95% oxygen, 5% carbon dioxide. Ex vivo experiments were then performed with the electrode inserted in the striatum of rat brain slices maintained in ACSF at 37 degrees C under 95% oxygen, 5% carbon dioxide. The results obtained from both in vitro and ex vivo experimentation indicate that the oxidation potential of peak 2 (DOPAC) is highly sensitive to changes in temperature and medium. Therefore the extrapolation from in vitro electrode calibrations performed in PBS at room temperature to ex vivo (brain slices) and possibly in vivo measurements of DOPAC oxidation should be reconsidered.
Crespi
F
F
Department of Pharmacology, Glaxo Research Laboratories, Verona, Italy.
D015102:3,4-Dihydroxyphenylacetic Acid / Q000032:analysis* / Q000378:metabolism; D000818:Animals; D001205:Ascorbic Acid / Q000032:analysis* / Q000378:metabolism; D001923:Brain Chemistry; D004566:Electrodes; D006897:Hydroxyindoleacetic Acid / Q000032:analysis* / Q000378:metabolism; D008297:Male; D010084:Oxidation-Reduction; D051381:Rats; D017208:Rats, Wistar; D013696:Temperature
D015102:3,4-Dihydroxyphenylacetic Acid; D006897:Hydroxyindoleacetic Acid; D001205:Ascorbic Acid