A gene-sized DNA molecule encoding heat-shock protein 70 in Oxytricha nova.
The gene-sized DNA molecule in the macronucleus (mac) of Oxytricha nova (On) encoding heat-shock protein 70 (Hsp70) was cloned and sequenced. It contains 2654 bp, including telomeres. It consists of a 394-bp A+T-rich 5' leader, a 1956-bp open reading frame (ORF) encoding a putative polypeptide of 651 amino acids (aa), and a 240-bp A+T-rich 3' trailer. The 5' leader contains two copies of the 13-bp heat-shock element (HSE) consensus sequence of other eukaryotes. On responds to heat and CdCl2 stress with a major increase in hsp70 transcripts. The presence of HSE in the nucleotide sequence of hsp70 and the stress-induced in hsp70 transcripts are the first evidence of conventional transcriptional regulation of a hypotrich gene-size DNA molecule. The ORF has large putative ATPase and polypeptide-binding domains with high aa identity to Hsp70 polypeptides of other eukaryotes.
Anderson
R C
RC
Department of Molecular, Cellular, University of Colorado, Boulder, 80309-0347, USA.
Lindauer
K R
KR
Prescott
D M
DM
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002104:Cadmium / Q000494:pharmacology; D019256:Cadmium Chloride; D002712:Chlorides / Q000494:pharmacology; D003001:Cloning, Molecular; D016384:Consensus Sequence / Q000235:genetics; D016054:DNA, Protozoan / Q000235:genetics*; D004587:Electrophoresis, Agar Gel; D017125:Genes, Protozoan; D018840:HSP70 Heat-Shock Proteins / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D016813:Oxytricha / Q000737:chemistry / Q000235:genetics*; D017421:Sequence Analysis; D013312:Stress, Physiological / Q000235:genetics; D014158:Transcription, Genetic / Q000235:genetics
D002712:Chlorides; D016054:DNA, Protozoan; D018840:HSP70 Heat-Shock Proteins; D002104:Cadmium; D019256:Cadmium Chloride