High-performance liquid chromatographic method for the analysis of imipramine metabolism in vitro by liver and brain microsomes.
A new sensitive method for resolution and quantitation metabolites of in vitro imipramine metabolism has been developed for use in liver and brain microsomes. Separation of metabolites was done using a Supelcosil PCN column with a mobile phase of acetonitrile-methanol-potassium phosphate dibasic (40:35:25, v/v/v), pH 7. Resolution is achieved for 2- and 10-hydroxyimipramine, and desipramine. Varying levels of these metabolites formed during in vitro incubations of rat liver and brain microsomes following treatments.
Sequeira
D J
DJ
Department of Biochemistry and Molecular Biology, University of Texas Medical School, Houston 77225, USA.
Strobel
H W
HW
D000818:Animals; D001921:Brain / Q000378:metabolism* / Q000648:ultrastructure; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000706:statistics & numerical data; D003891:Desipramine / Q000032:analysis; D006900:Hydroxylation; D007099:Imipramine / Q000031:analogs & derivatives / Q000032:analysis / Q000378:metabolism*; D008297:Male; D008861:Microsomes / Q000378:metabolism; D008862:Microsomes, Liver / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
C036691:didesipramine; C033803:2-hydroxyimipramine; D007099:Imipramine; D003891:Desipramine