title: Calcium-bound recoverin targets rhodopsin kinase to membranes to inhibit rhodopsin phosphorylation.
abstract: In rod photoreceptor cells, Ca2+-bound recoverin associates with disk membranes and inhibits light-dependent phosphorylation of rhodopsin. However, the functional significance of Ca2+-induced membrane association of recoverin has not been fully evaluated. We found that Ca2+-bound recoverin forms a complex with rhodopsin kinase preferentially at the membrane surface. Addition of increasing amounts of membranes promoted the membrane association of recoverin, and remarkably suppressed rhodopsin kinase activity. It was concluded that the Ca2+-recoverin-rhodopsin kinase complex is stabilized by membrane association, leading to effective suppression of the kinase activity.
authors.affiliation: Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Sanada
authors.forename: F
authors.initials: F
authors.lastname: Shimizu
authors.forename: K
authors.initials: K
authors.lastname: Kameyama
authors.forename: K
authors.initials: K
authors.lastname: Haga
authors.forename: T
authors.initials: T
authors.lastname: Haga
authors.forename: Y
authors.initials: Y
authors.lastname: Fukada
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism / Q000494:pharmacology; D002135:Calcium-Binding Proteins / Q000378:metabolism*; D002417:Cattle; D002462:Cell Membrane / Q000201:enzymology / Q000378:metabolism*; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005136:Eye Proteins; D051606:G-Protein-Coupled Receptor Kinase 1; D019204:GTP-Binding Proteins / Q000378:metabolism; D051597:Hippocalcin; D008074:Lipoproteins; D009419:Nerve Tissue Proteins; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism*; D051603:Recoverin; D012243:Rhodopsin / Q000378:metabolism*; D012374:Rod Cell Outer Segment / Q000378:metabolism* / Q000648:ultrastructure; D051552:beta-Adrenergic Receptor Kinases
chemical_list: D002135:Calcium-Binding Proteins; D005136:Eye Proteins; D008074:Lipoproteins; D009419:Nerve Tissue Proteins; D051603:Recoverin; D051597:Hippocalcin; D012243:Rhodopsin; D011494:Protein Kinases; D017868:Cyclic AMP-Dependent Protein Kinases; D051606:G-Protein-Coupled Receptor Kinase 1; D051552:beta-Adrenergic Receptor Kinases; D019204:GTP-Binding Proteins; D002118:Calcium