Globin digest, acidic protease hydrolysate, inhibits dietary hypertriglyceridemia and Val-Val-Tyr-Pro, one of its constituents, possesses most superior effect.
Globin digest (GD), prepared from globin by acidic protease treatment, suppressed the elevation of serum triglyceride level in not only total but also chylomicron fraction after oral administration of olive oil. By screening with this lowering activity, we concluded that Val-Val-Tyr-Pro (VVYP) would be most effective constituent having hypotriglyceridemic action in GD. The mode of their action was dose dependent and did not show species specificity. Neither the repression of peristaltic movement of intestine nor the delaying of gastric emptying was caused by intake of GD or VVYP, however, the excretion of administered lipid was much more than that of control. Furthermore, administration of GD caused more prominent activation of hepatic triglyceride lipase (HTGL) and the increase of hepatic free fatty acid (FFA) concentration in early phase after administration of fat. From these results, it could be elucidated that GD, and also VVYP, inhibited fat absorption from digestive tract and enhanced activity of HTGL, so that more rapid clearance of dietary hypertriglyceridemia was caused.
Kagawa
K
K
Pharmacological Laboratory, Hankyu-Kyoei Bussan Co., Ltd., Osaka, Japan.
Matsutaka
H
H
Fukuhama
C
C
Watanabe
Y
Y
Fujino
H
H
D000595:Amino Acid Sequence; D000818:Animals; D004041:Dietary Fats / Q000008:administration & dosage*; D004285:Dogs; D010450:Endopeptidases / Q000378:metabolism*; D005260:Female; D005914:Globins / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D015228:Hypertriglyceridemia / Q000209:etiology / Q000517:prevention & control*; D008071:Lipoprotein Lipase / Q000097:blood; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar
D004041:Dietary Fats; D009842:Oligopeptides; C099540:valyl-valyl-tyrosyl-proline; D005914:Globins; D008071:Lipoprotein Lipase; D010450:Endopeptidases