The C-C chemokine MCP-1 differentially modulates the avidity of beta 1 and beta 2 integrins on T lymphocytes.
The ability of chemokines, particularly MCP-1, to induce integrin-dependent binding of T lymphocytes to endothelial adhesion molecules or extracellular matrix (ECM) components was examined. MCP-1 induced significant adhesion to fibronectin (FN) and to endothelial-secreted ECM but not to purified ICAM-1 or VCAM-1, or to activated endothelium. The MCP-1-induced binding of T lymphocytes to FN was rapid, dose dependent, and resulted from activation of both VLA-4 and VLA-5. Like MCP-1, the chemokines RANTES and MIP-1 beta induced T lymphocyte binding to FN, but not to ICAM-1. We suggest therefore, that these T lymphocyte chemokines may be most important, not in initiating integrin-dependent firm adhesion of T lymphocytes to the vascular wall, but rather, in subsequent adhesive interactions during migration into tissue.
Carr
M W
MW
The Center for Blood Research, Harvard Medical School Department of Pathology, Boston, Massachusetts 02115, USA.
Alon
R
R
Springer
T A
TA
D018821:CD18 Antigens / Q000187:drug effects / Q000378:metabolism*; D002448:Cell Adhesion / Q000187:drug effects / Q000276:immunology; D002478:Cells, Cultured; D018932:Chemokine CCL2 / Q000276:immunology / Q000494:pharmacology*; D004730:Endothelium, Vascular; D005109:Extracellular Matrix / Q000378:metabolism; D006801:Humans; D019012:Integrin beta1 / Q000187:drug effects / Q000378:metabolism*; D016023:Integrins / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism*; D018799:Intercellular Adhesion Molecule-1 / Q000494:pharmacology; D011485:Protein Binding / Q000187:drug effects / Q000276:immunology; D013601:T-Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D014471:Umbilical Veins; D019010:Vascular Cell Adhesion Molecule-1 / Q000494:pharmacology
D018821:CD18 Antigens; D018932:Chemokine CCL2; D019012:Integrin beta1; D016023:Integrins; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1