title: Assembly of T7 capsids from independently expressed and purified head protein and scaffolding protein.
abstract: Prohead-like capsid shells containing the scaffolding and head proteins of bacteriophage T7 were isolated after both proteins were expressed from the cloned genes in the same cell. When the head-tail connector protein was also expressed, the isolated capsids contained neither connector nor scaffolding protein and resembled mature phage capsids rather than proheads. However, only a small fraction of the head protein was converted to stable capsid structures in either case. Purified scaffolding protein (expressed individually from the cloned gene) appeared to be a monomer in solution; purified head protein appeared to be a tetramer. The purified proteins reacted in the presence of polyethylene glycol or dextran to produce prohead-like capsid shells and also polycapsids consisting primarily of head protein, similar to the polycapsids observed after infection by T7 mutants lacking connector or core proteins. Neither capsids nor polycapsids were produced in the absence of scaffolding protein. Polycapsids were usually the predominant product even when scaffolding protein was in excess, and a small fraction of scaffolding protein catalyzed the conversion of an excess of head protein to polycapsids. Our results suggest that the first step in the natural pathway to prohead formation is the assembly of incomplete prohead shells, which are normally closed by insertion of a connector-core complex. In the absence of a functional connector-core complex, incomplete capsid shells apparently react further to form polycapsids or completely closed capsid shells.
authors.affiliation: Biology Department, Brookhaven National Laboratory, Upton, NY 11973, USA.
authors.forename: M E
authors.initials: ME
authors.lastname: Cerritelli
authors.forename: F W
authors.initials: FW
authors.lastname: Studier
mesh_terms: D017123:Bacteriophage T7 / Q000502:physiology* / Q000648:ultrastructure; D002213:Capsid / Q000378:metabolism*; D005591:Chemical Fractionation; D003001:Cloning, Molecular; D014764:Viral Proteins / Q000378:metabolism; D019065:Virus Assembly
chemical_list: D014764:Viral Proteins