Behavioral and cellular effects of serotonin on locomotion and male mating posture in Ascaris suum (Nematoda).
The site and mode of action of serotonin on locomotion were investigated in the parasitic nematode Ascaris suum. Injection of serotonin into Ascaris immediately caused paralysis in animals that were generating locomotory waveforms. Injected serotonin also increased body length and decreased the number of propagating body waves. Similar injections into the male tail produced a ventral tail curl. Injection of N-acetyl-serotonin had no effect on the generation of locomotory waveforms, but increased the body length and decreased the number of body waves in the waveform. Other biogenic amines were also tested but were much less potent. Serotonin decreased the amplitude of a submaximal acetylcholine-induced muscle contraction and increased the time to attain this contraction. The time course of this effect on the response to ACh was much slower than the action of injected serotonin on locomotory waveforms, suggesting that additional elements are involved in the action of serotonin on locomotory behavior. Serotonin abolished spontaneous slow potentials in VI motor neurons and decreased the frequency of EPSPs in DE2 motor neurons, probably by a pre-synaptic mechanism. In the male tail, serotonin depolarized the male-specific transverse ventral muscle cells, but did not affect either dorsal or ventral longitudinal muscle cells.
Reinitz
C A
CA
Neuroscience Training Program, University of Wisconsin, Madison 53706, USA.
Stretton
A O
AO
D000818:Animals; D017165:Ascaris suum; D004305:Dose-Response Relationship, Drug; D005260:Female; D008124:Locomotion / Q000187:drug effects*; D008297:Male; D009046:Motor Neurons / Q000502:physiology*; D009348:Nematoda; D011187:Posture / Q000502:physiology; D012701:Serotonin / Q000494:pharmacology*; D012726:Sexual Behavior, Animal / Q000187:drug effects*
D012701:Serotonin