title: Sequence of the Bacillus stearothermophilus gene encoding aspartokinase II.
abstract: The complete nucleotide sequence of the gene encoding aspartokinase (Ask) II from thermophilic Bacillus stearothermophilus has been determined. Degenerate oligodeoxyribonucleotides primed the amplification of a 932-bp gene. This sequence was successively used for constructing new primers applied in inverse polymerase chain reaction using, as template, self-ligating DNA fragments. The deduced amino-acid sequence is 68.7% identical with the sequence of the Bacillus sp. strain MGA3 Ask II.
authors.affiliation: Dipartimento di Genetica e Microbiologia, University of Pavia, Italy.
authors.forename: R
authors.initials: R
authors.lastname: Cantoni
authors.forename: M
authors.initials: M
authors.lastname: Labó
authors.forename: E
authors.initials: E
authors.lastname: De Rossi
authors.forename: G
authors.initials: G
authors.lastname: Riccardi
mesh_terms: D000595:Amino Acid Sequence; D001222:Aspartate Kinase / Q000235:genetics*; D001483:Base Sequence; D017931:DNA Primers / Q000737:chemistry; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D001411:Geobacillus stearothermophilus / Q000201:enzymology / Q000235:genetics*; D008969:Molecular Sequence Data
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial; D001222:Aspartate Kinase