title: 32P-postlabelling of bulky human DNA adducts enriched by different methods including immunoaffinity chromatography.
abstract: DNA adducts in lymphocytes and granulocytes of men exposed occupationally and environmentally to high concentrations of aromatic compounds in air were measured by the 32P-postlabelling method. Adducts in the same samples were characterized using nuclease P1 enrichment, butanol extraction and immunoaffinity purification with an antiserum raised against benzo[alpha]pyrene diol epoxide (BPDE). Only part of the adducts found in human samples were extracted by butanol. It also seemed, that only a small part of them belonged to the group of polycyclic aromatic hydrocarbons (PAHs) recognised by the antibody. Relative content of hydrophobic adducts and those with a structure similar to PAHs was higher in winter samples (when exposure to aromatic chemicals in air was higher) in comparison to samples collected in summer.
authors.affiliation: Center for Nutrition and Toxicology, Karolinska Institute, Huddinge, Sweden.
authors.forename: P
authors.initials: P
authors.lastname: Widlak
authors.forename: E
authors.initials: E
authors.lastname: Grzybowska
authors.forename: K
authors.initials: K
authors.lastname: Hemminki
authors.forename: R
authors.initials: R
authors.lastname: Santella
authors.forename: M
authors.initials: M
authors.lastname: Chorazy
mesh_terms: D015123:7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide / Q000032:analysis / Q000494:pharmacology; D000393:Air Pollutants / Q000378:metabolism / Q000633:toxicity*; D002273:Carcinogens / Q000494:pharmacology; D002846:Chromatography, Affinity; D002855:Chromatography, Thin Layer; D004247:DNA / Q000032:analysis / Q000378:metabolism; D018736:DNA Adducts / Q000032:analysis / Q000737:chemistry*; D003848:Deoxyguanine Nucleotides / Q000378:metabolism; D006098:Granulocytes / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D007164:Immunosorbents / Q000276:immunology / Q000378:metabolism; D008214:Lymphocytes / Q000737:chemistry / Q000378:metabolism; D008297:Male; D016273:Occupational Exposure; D011044:Poland; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis / Q000737:chemistry*; D012621:Seasons; D015719:Single-Strand Specific DNA and RNA Endonucleases / Q000378:metabolism
chemical_list: D000393:Air Pollutants; D002273:Carcinogens; D018736:DNA Adducts; D003848:Deoxyguanine Nucleotides; D007164:Immunosorbents; D011084:Polycyclic Aromatic Hydrocarbons; C095911:polycyclic aromatic hydrocarbons-DNA adduct; D015123:7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide; C007257:2'-deoxyguanosine 5'-phosphate; D004247:DNA; D015719:Single-Strand Specific DNA and RNA Endonucleases