title: Expression of growth hormone receptors in murine lymphoid cells analyzed by flow cytofluorometry.
abstract: We have analyzed the expression of GH receptors (GHR) in murine lymphoid organs using flow cytofluorometry with biotinylated bovine GH and specific fluorescein isothiocyanate-labeled monoclonal antibodies defining distinct lymphoid cell populations. GHR were widely expressed in al murine hematopoietic tissues and in fetuses, newborns, and 3- and 7-week-old animals. In the bone marrow, all hematopoietic lineages expressed variable levels of GH receptors, whereas in the thymus, this expression was mainly seen in CD4-, CD8-, CD4+CD8+, and CD8+ subpopulations. At the periphery, 50% of splenocytes and peripheral blood lymphocytes and 20% of lymph node cells were GHR positive, with a wider receptor expression on B cells and macrophages (approximately 50%) than on T cells (approximately 20%), where the labeling was seen on both CD4+ and CD8+ cell subsets. Interestingly, the proportion of GHR-bearing CD4+ and CD8+ splenocytes significantly increased after T cell activation with Concanavalin A or anti-CD3. Finally, we demonstrated that all peripheral T cells expressing GHR also expressed PRL receptors. Our study provides a molecular basis to study the factors controlling GHR expression and to better understand the influence of GH in the regulation of immune function.
authors.affiliation: CNRS URA 1461, Université Paris V, Hôpital Necker, France.
authors.forename: M C
authors.initials: MC
authors.lastname: Gagnerault
authors.forename: M C
authors.initials: MC
authors.lastname: Postel-Vinay
authors.forename: M
authors.initials: M
authors.lastname: Dardenne
mesh_terms: D000818:Animals; D001710:Biotin; D015496:CD4-Positive T-Lymphocytes / Q000737:chemistry; D018414:CD8-Positive T-Lymphocytes / Q000737:chemistry; D005314:Embryonic and Fetal Development; D005260:Female; D005434:Flow Cytometry; D013006:Growth Hormone / Q000378:metabolism; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000737:chemistry / Q000378:metabolism; D008221:Lymphoid Tissue / Q000737:chemistry* / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D011981:Receptors, Prolactin / Q000032:analysis; D011986:Receptors, Somatotropin / Q000032:analysis* / Q000378:metabolism; D013154:Spleen / Q000737:chemistry / Q000196:embryology; D013601:T-Lymphocytes / Q000737:chemistry; D014018:Tissue Distribution
chemical_list: D011981:Receptors, Prolactin; D011986:Receptors, Somatotropin; D001710:Biotin; D013006:Growth Hormone