Mechanical effects on the kinetics of the HIV proteinase deactivation.
The proteinase from HIV undergoes rapid and irreversible deactivation caused by mild mechanical stirring. Both the free enzyme and the ternary Michaelis complex disappear in two separate first-order processes, with half-times of 3.0 and 0.8 minutes, respectively. Ignoring these deactivation steps distorts the results of kinetic analyses.
Kuzmic
P
P
School of Pharmacy, Department of Chemistry, University of Wisconsin, Madison 53706, USA.
Peranteau
A G
AG
García-Echeverría
G
G
Rich
D H
DH
D000595:Amino Acid Sequence; D002384:Catalysis; D016333:HIV Protease / Q000378:metabolism*; D017320:HIV Protease Inhibitors / Q000494:pharmacology*; D006868:Hydrolysis; D007700:Kinetics; D008969:Molecular Sequence Data; D013379:Substrate Specificity
D017320:HIV Protease Inhibitors; D016333:HIV Protease