32P-postlabelling of bulky human DNA adducts enriched by different methods including immunoaffinity chromatography.
DNA adducts in lymphocytes and granulocytes of men exposed occupationally and environmentally to high concentrations of aromatic compounds in air were measured by the 32P-postlabelling method. Adducts in the same samples were characterized using nuclease P1 enrichment, butanol extraction and immunoaffinity purification with an antiserum raised against benzo[alpha]pyrene diol epoxide (BPDE). Only part of the adducts found in human samples were extracted by butanol. It also seemed, that only a small part of them belonged to the group of polycyclic aromatic hydrocarbons (PAHs) recognised by the antibody. Relative content of hydrophobic adducts and those with a structure similar to PAHs was higher in winter samples (when exposure to aromatic chemicals in air was higher) in comparison to samples collected in summer.
Widlak
P
P
Center for Nutrition and Toxicology, Karolinska Institute, Huddinge, Sweden.
Grzybowska
E
E
Hemminki
K
K
Santella
R
R
Chorazy
M
M
D015123:7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide / Q000032:analysis / Q000494:pharmacology; D000393:Air Pollutants / Q000378:metabolism / Q000633:toxicity*; D002273:Carcinogens / Q000494:pharmacology; D002846:Chromatography, Affinity; D002855:Chromatography, Thin Layer; D004247:DNA / Q000032:analysis / Q000378:metabolism; D018736:DNA Adducts / Q000032:analysis / Q000737:chemistry*; D003848:Deoxyguanine Nucleotides / Q000378:metabolism; D006098:Granulocytes / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D007164:Immunosorbents / Q000276:immunology / Q000378:metabolism; D008214:Lymphocytes / Q000737:chemistry / Q000378:metabolism; D008297:Male; D016273:Occupational Exposure; D011044:Poland; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis / Q000737:chemistry*; D012621:Seasons; D015719:Single-Strand Specific DNA and RNA Endonucleases / Q000378:metabolism
D000393:Air Pollutants; D002273:Carcinogens; D018736:DNA Adducts; D003848:Deoxyguanine Nucleotides; D007164:Immunosorbents; D011084:Polycyclic Aromatic Hydrocarbons; C095911:polycyclic aromatic hydrocarbons-DNA adduct; D015123:7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide; C007257:2'-deoxyguanosine 5'-phosphate; D004247:DNA; D015719:Single-Strand Specific DNA and RNA Endonucleases