title: Mutational analysis of the mengovirus poly(C) tract and surrounding heteropolymeric sequences.
abstract: Previously, we described three mengovirus mutants derived from cDNA plasmids, containing shortened poly(C) tracts (C8, C12, and C13UC10), that exhibited strong attenuation for virulence in mice yet grew like wild-type virus in HeLa cells. Thirteen additional mutants hav now been constructed and characterized. Five of these differ only in poly(C) length, including one with a precise deletion of the tract. The other mutants bear deletions into the regions juxtaposing poly(C). Studies with HeLa cells confirm the essential dispensability of mengovirus's poly(C) tract but reveal a subtle, measurable correlation between poly(C) length and plaque diameter. Virulence studies with mice also revealed a strong correlation between poly(C) length and virulence. For the poly(C)-flanking mutations, the 15 bases directly 5' of the tract proved dispensable for virus viability, whereas the 20 to 30 bases 3' of poly(C) were critical for growth, thus implicating this region in the basal replication of the virus.
authors.affiliation: Institute for Molecular Virology, University of Wisconsin, Madison 53706, USA. LRM@AHABS.wisc.edu
authors.forename: L R
authors.initials: LR
authors.lastname: Martin
authors.forename: G M
authors.initials: GM
authors.lastname: Duke
authors.forename: J E
authors.initials: JE
authors.lastname: Osorio
authors.forename: D J
authors.initials: DJ
authors.lastname: Hall
authors.forename: A C
authors.initials: AC
authors.lastname: Palmenberg
mesh_terms: D000818:Animals; D001483:Base Sequence; D018188:Cardiovirus Infections / Q000821:virology; D003001:Cloning, Molecular; D046508:Culture Techniques; D018076:DNA, Complementary; D004279:DNA, Viral; D005260:Female; D006367:HeLa Cells; D006801:Humans; D008574:Mengovirus / Q000235:genetics* / Q000378:metabolism / Q000472:pathogenicity; D051379:Mice; D008807:Mice, Inbred BALB C; D008813:Mice, Inbred ICR; D008969:Molecular Sequence Data; D016296:Mutagenesis; D009690:Nucleic Acid Conformation; D011066:Poly C / Q000235:genetics; D012367:RNA, Viral / Q000378:metabolism; D014774:Virulence / Q000235:genetics; D014779:Virus Replication / Q000235:genetics
chemical_list: D018076:DNA, Complementary; D004279:DNA, Viral; D012367:RNA, Viral; D011066:Poly C