title: Rabbit translation elongation factor 1 alpha stimulates the activity of homologous aminoacyl-tRNA synthetase.
abstract: Functional and structural sequestration of aminoacyl-tRNA has been recently found in eukaryotic cells and the aminoacyl-tRNA channeling has been suggested [B.S. Negrutskii et al., Proc. Natl. Acad. Sci. 91 (1994) 964-968], but molecular details and mechanism of the process remained unclear. In this paper we have verified a possible interaction between rabbit aminoacyl-tRNA synthetase and homologous translation elongation factor 1 alpha (EF-1 alpha), the proteins which may play a role of sequential components involved in the transfer of the aminoacyl-tRNA along the protein synthetic metabolic chain. The stimulation of the phenylalanyl-tRNA synthetase activity by EF-1 alpha is found. The effect is shown to be specific towards the origin of tRNA and elongation factor molecules. The data obtained favor the direct transfer mechanism of the aminoacyl-tRNA channeling process during eukaryotic protein synthesis.
authors.affiliation: Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Kiev.
authors.forename: B S
authors.initials: BS
authors.lastname: Negrutskii
authors.forename: T V
authors.initials: TV
authors.lastname: Budkevich
authors.forename: V F
authors.initials: VF
authors.lastname: Shalak
authors.forename: G V
authors.initials: GV
authors.lastname: Turkovskaya
authors.forename: A V
authors.initials: AV
authors.lastname: El'Skaya
mesh_terms: D000818:Animals; D006153:Guanosine Diphosphate / Q000378:metabolism; D007700:Kinetics; D008099:Liver / Q000378:metabolism; D020648:Peptide Elongation Factor 1; D010444:Peptide Elongation Factor Tu / Q000494:pharmacology; D010445:Peptide Elongation Factors / Q000378:metabolism* / Q000494:pharmacology; D010652:Phenylalanine-tRNA Ligase / Q000378:metabolism*; D012360:RNA, Transfer, Phe / Q000096:biosynthesis; D011817:Rabbits; D046249:Transfer RNA Aminoacylation / Q000502:physiology; D015003:Yeasts
chemical_list: D020648:Peptide Elongation Factor 1; D010445:Peptide Elongation Factors; D012360:RNA, Transfer, Phe; D006153:Guanosine Diphosphate; D010444:Peptide Elongation Factor Tu; D010652:Phenylalanine-tRNA Ligase