Evidence that two genomic species of Rhizobium are associated with Medicago truncatula.
Seventy-three isolates of rhizobia sampled from root nodules of Medicago truncatula were analyzed by restriction fragment length polymorphism (RFLP) of DNA regions amplified by the polymerase chain reaction (PCR) targeting the symbiotic plasmid (nifD-K, nodD1, and nodD2 genes) and the chromosome (16S rDNA plus intergenic spacer). Two genotypic groups were found, regardless of the DNA region targeted. These two groups were given the status of genomic species based on results of DNA/DNA hybridization.
Rome
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S
Laboratoire d'Ecologie Microbienne du Sol, URA-CNRS 1977, Université Lyon I, Villeurbanne, France.
Brunel
B
B
Normand
P
P
Fernandez
M
M
Cleyet-Marel
J C
JC
D001483:Base Sequence; D002876:Chromosomes, Bacterial / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D016680:Genome, Bacterial; D005838:Genotype; D000455:Medicago sativa / Q000382:microbiology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012231:Rhizobium / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D013045:Species Specificity
D017931:DNA Primers; D004269:DNA, Bacterial; D004275:DNA, Ribosomal