title: Composition of Pneumocystis carinii neutral lipids and identification of coenzyme Q10 as the major ubiquinone homolog.
abstract: The lipids of purified preparations of Pneumocystis carinii carinii freshly isolated from infected rats were analyzed and compared with those of whole lungs from normal and methylprednisolone-immunosuppressed uninfected rats. In this study, the neutral lipid fraction was examined in detail; the relative concentrations of individual classes making up this fraction were quantified. Of particular interest was the nature of the organism's ubiquinone (coenzyme Q, CoQ) fraction because atovaquone, a hydroxynaphtho-quinone (566C80) analog of ubiquinone, is efficacious in the treatment of P. carinii pneumonia. The ubiquinone concentration in both P. carinii and lung tissues was relatively low compared to that present in rat heart and liver tissues. Two homologs were identified in the organism: CoQ10 was the predominant homolog with lesser amounts of CoQ9 present. In contrast, the lungs of normal and immunosuppressed uninfected rats had CoQ9 and lesser amounts of CoQ8, but no detectable CoQ10. Furthermore, radiolabeled mevalonic acid was incorporated in vitro into the ubiquinone fraction of P. carinii indicating that the organism has the de novo branch of the isoprenoid biosynthetic pathway leading to polyprenyl formation. Hence, it was concluded that CoQ10 (if not both CoQ10 and CoQ9) in P. carinii was not scavenged from the host but was synthesized by the organism. Although lung tissues contained substantial free fatty acids, the organism was enriched in these lipids. The high concentration of free fatty acids and relatively low level of triglycerides in P. carinii suggest that fatty acids may represent major carbon sources for ATP production by the organism.
authors.affiliation: Department of Biological Sciences, University of Cincinnati, Ohio 45221, USA.
authors.forename: J E
authors.initials: JE
authors.lastname: Ellis
authors.forename: M A
authors.initials: MA
authors.lastname: Wyder
authors.forename: L
authors.initials: L
authors.lastname: Zhou
authors.forename: A
authors.initials: A
authors.lastname: Gupta
authors.forename: H
authors.initials: H
authors.lastname: Rudney
authors.forename: E S
authors.initials: ES
authors.lastname: Kaneshiro
mesh_terms: D000818:Animals; D003067:Coenzymes; D008055:Lipids / Q000032:analysis* / Q000096:biosynthesis; D008168:Lung / Q000382:microbiology; D015394:Molecular Structure; D011010:Pneumocystis / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D011020:Pneumonia, Pneumocystis / Q000382:microbiology; D051381:Rats; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000032:analysis / Q000096:biosynthesis
chemical_list: D003067:Coenzymes; D008055:Lipids; D014451:Ubiquinone; C024989:coenzyme Q10