Blue and ultraviolet light-absorbing opsin from the retinal pigment epithelium.
The retinal pigment epithelium (RPE) contains an abundant opsin that is distinct from rhodopsin and cone visual pigments and is able to bind the retinaldehyde chromophore. The putative retinal G protein-coupled receptor (RGR) was isolated in digitonin solution from bovine RPE microsomes and copurified consistently with a minor 34-kDa protein. The absorption spectrum of RGR revealed endogenous pH-sensitive absorbance in the blue and near-ultraviolet regions of light. Membrane-bound RGR was incubated with exogenously added all-trans-retinal and formed two long-lived pH-dependent photopigments with absorption maxima of 469 +/- 2.4 and 370 +/- 7.3 nm. The effects of hydrogen ion concentration suggest that the blue and near-UV photopigments are tautomeric forms of RGR, in which an all-trans-retinal Schiff base is protonated or unprotonated, respectively. The RPE pigment was also demonstrable by its reactivity to hydroxylamine in the dark. The retinaldehyde-RGR conjugate at neutral pH favors the near-UV pigment and is a novel light-absorbing opsin in the vertebrate eye.
Hao
W
W
Department of Microbiology, University of Southern California School of Medicine, Los Angeles, USA.
Fong
H K
HK
D000818:Animals; D002417:Cattle; D005136:Eye Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000528:radiation effects; D019204:GTP-Binding Proteins / Q000302:isolation & purification; D006863:Hydrogen-Ion Concentration; D019811:Hydroxylamine; D006898:Hydroxylamines; D066298:In Vitro Techniques; D008027:Light; D010857:Pigment Epithelium of Eye / Q000737:chemistry* / Q000528:radiation effects*; D011956:Receptors, Cell Surface / Q000737:chemistry* / Q000302:isolation & purification / Q000528:radiation effects; D043562:Receptors, G-Protein-Coupled; D012172:Retinaldehyde / Q000737:chemistry / Q000528:radiation effects; D017299:Rod Opsins / Q000737:chemistry* / Q000302:isolation & purification / Q000528:radiation effects*; D012545:Schiff Bases / Q000302:isolation & purification; D013053:Spectrophotometry; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism; D014466:Ultraviolet Rays
D005136:Eye Proteins; C089754:G protein-coupled receptor RGR; D006898:Hydroxylamines; D011956:Receptors, Cell Surface; D043562:Receptors, G-Protein-Coupled; D017299:Rod Opsins; D012545:Schiff Bases; D019811:Hydroxylamine; D019204:GTP-Binding Proteins; D012172:Retinaldehyde