Property of receptor for vasoactive intestinal contractor (VIC) expressed in Xenopus oocytes injected with mRNA from rat intestine.
Property of receptor for vasoactive intestinal contractor (VIC), a peptide related to the endothelin family, expressed in Xenopus oocytes by injecting mRNA obtained from the intestine of rat, were studied using the voltage-clamp method. Inward-current responses to VIC (1 nM-100 nM) were evoked in a concentration-dependent manner in mRNA-injected oocytes. Non-injected and water-injected oocytes failed to respond to VIC. The reversal potential for the VIC response was around -20 mV and the depolarizing shift was approximately 18 mV, when the external concentration of Cl-was halved, in agreement with the Nernst equation. The response to VIC was suppressed either by the external application to BAPTA/AM (10 microM) or by pertussis toxin 0.5 microgram/ml). These results indicate that the receptor for VIC, functionally expressed in Xenopus oocytes injected with rat intestinal mRNA, is coupled to pertussis toxin-sensitive G-protein and its activation leads to mobilization of intracellular Ca2+.
Yoshimura
M
M
Department of Pharmacology II, Nagasaki University School of Medicine, Japan.
Yoshida
S
S
Taniyama
K
K
D000818:Animals; D018118:Chloride Channels / Q000502:physiology; D002712:Chlorides / Q000378:metabolism; D003001:Cloning, Molecular; D008297:Male; D009865:Oocytes; D037342:Pertussis Toxin; D012333:RNA, Messenger / Q000008:administration & dosage / Q000235:genetics*; D051381:Rats; D018000:Receptors, Peptide / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D010566:Virulence Factors, Bordetella / Q000494:pharmacology; D014982:Xenopus laevis
D018118:Chloride Channels; D002712:Chlorides; D012333:RNA, Messenger; D018000:Receptors, Peptide; D010566:Virulence Factors, Bordetella; C071686:vasoactive intestinal contractor receptor; D037342:Pertussis Toxin