Sequence of the Bacillus stearothermophilus gene encoding aspartokinase II.
The complete nucleotide sequence of the gene encoding aspartokinase (Ask) II from thermophilic Bacillus stearothermophilus has been determined. Degenerate oligodeoxyribonucleotides primed the amplification of a 932-bp gene. This sequence was successively used for constructing new primers applied in inverse polymerase chain reaction using, as template, self-ligating DNA fragments. The deduced amino-acid sequence is 68.7% identical with the sequence of the Bacillus sp. strain MGA3 Ask II.
Cantoni
R
R
Dipartimento di Genetica e Microbiologia, University of Pavia, Italy.
Labó
M
M
De Rossi
E
E
Riccardi
G
G
D000595:Amino Acid Sequence; D001222:Aspartate Kinase / Q000235:genetics*; D001483:Base Sequence; D017931:DNA Primers / Q000737:chemistry; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D001411:Geobacillus stearothermophilus / Q000201:enzymology / Q000235:genetics*; D008969:Molecular Sequence Data
D017931:DNA Primers; D004269:DNA, Bacterial; D001222:Aspartate Kinase