title: Soluble interleukin-2 receptor alpha is elevated in sera of patients with benign ovarian neoplasms and epithelial ovarian cancer.
abstract: BACKGROUND
  Previous studies have established that soluble interleukin-2 receptor alpha (sIL-2R alpha) levels are elevated in ascites and sera from individuals with advanced ovarian cancer (International Federation of Gynecology and Obstetrics [FIGO] Stage III/IV). This study was undertaken to evaluate sIL-2R alpha levels in individuals with benign ovarian neoplasms and early stage ovarian cancer (FIGO Stage I/II). Comparison with CA 125 levels was performed to assess screening potential.
  
  
  METHODS
  Sera from 92 healthy individuals, 61 with benign adnexal masses, 12 patients with FIGO Stage I/II ovarian cancers, and 27 patients with FIGO Stage III/IV ovarian cancers were assayed for sIL-2R alpha by enzyme-linked immunosorbent assay and CA 125 by radioimmunoassay.
  
  
  RESULTS
  The mean serum sIL-2R alpha levels for benign pelvic masses, and Stage I/II and Stage III/IV epithelial ovarian cancer were 1507 +/- 82, 1631 +/- 274, and 2596 +/- 384 U/ml, respectively. The difference between mean serum sIL-2R alpha levels in individuals with benign adnexal masses and Stage III/IV epithelial ovarian cancer was statistically significant (P < 0.05). In addition, of the four individuals with FIGO Stage I/II ovarian cancer who had CA125 levels below 35 U/ml, the accepted upper limit of normal, three patients had elevated serum sIL-2R alpha levels. Eleven of 12 patients (92%) with potentially curable Stage I/II disease had elevated serum levels of either sIL-2R alpha or CA125 and 8 of 12 (67%) had elevations of both sIL-2R alpha and CA125. Sensitivity and specificity of a combination of CA 125 and soluble IL-2R alpha were 88.5% and 27.1%, respectively.
  
  
  CONCLUSIONS
  Soluble interleukin-2 receptor alpha levels do not appear to differentiate between benign adnexal lesions and early malignancy; however, measurement of sIL-2R alpha levels in combination with CA125 warrants further evaluation to determine if together they will identify individuals with Stages I and II ovarian cancer.
authors.affiliation: Division of Oncology Research, Toronto General Hospital, Ontario, Canada.
authors.forename: J A
authors.initials: JA
authors.lastname: Hurteau
authors.forename: R P
authors.initials: RP
authors.lastname: Woolas
authors.forename: I J
authors.initials: IJ
authors.lastname: Jacobs
authors.forename: D C
authors.initials: DC
authors.lastname: Oram
authors.forename: C C
authors.initials: CC
authors.lastname: Kurman
authors.forename: L A
authors.initials: LA
authors.lastname: Rubin
authors.forename: D L
authors.initials: DL
authors.lastname: Nelson
authors.forename: A
authors.initials: A
authors.lastname: Berchuck
authors.forename: R C
authors.initials: RC
authors.lastname: Bast
authors.forename: G B
authors.initials: GB
authors.lastname: Mills
mesh_terms: D014408:Biomarkers, Tumor / Q000097:blood*; D018394:CA-125 Antigen / Q000097:blood; D002277:Carcinoma / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D010051:Ovarian Neoplasms / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D011237:Predictive Value of Tests; D011863:Radioimmunoassay; D015375:Receptors, Interleukin-2 / Q000378:metabolism*; D012680:Sensitivity and Specificity; D012995:Solubility
chemical_list: D014408:Biomarkers, Tumor; D018394:CA-125 Antigen; D015375:Receptors, Interleukin-2