A new enzymatic method for the measurement of creatinine involving a novel ATP-dependent enzyme, N-methylhydantoin amidohydrolase.
A new enzymatic method for the measurement of serum and urine creatinine is described. The method is based on a novel microbial creatinine degradation pathway via N-methylhydantoin [Shimizu et al., Clin. Chim. Acta, 185, 241-252 (1989)]. By using two novel enzymes, N-methylhydantoin amidohydrolase and N-carbamoylsarcosine amidohydrolase, as key enzymes, coupled with a colorimetric procedure for hydrogen peroxide detection, the creatinine level can be measured. The results obtained for human serum and urine show good correlation with those obtained by a standard chemical method based on the Jaffe reaction. The new method is simple and specific, and shows excellent sensitivity and reliability.
Ogawa
J
J
Department of Agricultural Chemistry, Kyoto University, Japan.
Nirdnoy
W
W
Tabata
M
M
Yamada
H
H
Shimizu
S
S
D000255:Adenosine Triphosphate / Q000378:metabolism*; D000581:Amidohydrolases / Q000378:metabolism*; D000641:Ammonia / Q000378:metabolism; D003124:Colorimetry / Q000379:methods; D003401:Creatine / Q000378:metabolism; D003404:Creatinine / Q000097:blood* / Q000652:urine*; D006801:Humans; D012680:Sensitivity and Specificity
D000641:Ammonia; D000255:Adenosine Triphosphate; D003404:Creatinine; D000581:Amidohydrolases; C051522:N-methylhydantoin amidohydrolase; D003401:Creatine