Characterization of a cDNA encoding cysteine proteinase inhibitor from Chinese cabbage (Brassica campestris L. ssp. pekinensis) flower buds.
A cDNA encoding a new phytocystatin isotype named BCPI-1 was isolated from a cDNA library of Chinese cabbage flower buds. The BCPI-1 clone encodes 199 amino acids resulting in a protein much larger than other known phytocystatins. BCPI-1 has an unusually long C-terminus. A BCPI-1 fusion protein expressed in Escherichia coli strongly inhibits the enzymatic activity of papain, a cysteine proteinase. Genomic Southern blot analysis revealed that the BCPI gene is a member of a small multi-gene family in Chinese cabbage. Northern blot analysis showed that it is differentially expressed in the flower bud, leaf and root.
Lim
C O
CO
Plant Molecular Biology and Biotechnology Research Center, Chinju, Korea.
Lee
S I
SI
Chung
W S
WS
Park
S H
SH
Hwang
I
I
Cho
M J
MJ
D000595:Amino Acid Sequence; D001483:Base Sequence; D001937:Brassica / Q000235:genetics*; D015853:Cysteine Proteinase Inhibitors / Q000096:biosynthesis / Q000235:genetics* / Q000494:pharmacology; D018076:DNA, Complementary / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D018628:Gene Dosage; D017343:Genes, Plant; D008969:Molecular Sequence Data; D005810:Multigene Family; D010206:Papain / Q000037:antagonists & inhibitors; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000494:pharmacology; D018520:Plant Shoots; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000494:pharmacology; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
C099228:BCPI-1 protein, Brassica rapa; D015853:Cysteine Proteinase Inhibitors; D018076:DNA, Complementary; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; D010206:Papain