Delay in manifestations of aging by grafting NGF cultured chromaffin cells in adulthood.
Dopamine agonists or grafts compensate impaired motor functions in aged rats. However, there is no evidence showing whether grafting in adulthood retard aging manifestations. Motor performance of 13-month-old rats was tested on 2 meter-long wooden beams which had a 15 degree inclination and whose thickness varied from 3, 6, 12, 18, to 24 mm. Rats at 14 months were randomly assigned to 3 groups: sham graft (Group 1); intrastriatal graft of chromaffin cells cultured with NGF (Group 2); intrastriatal graft of chromaffin cells (Group 3). Motor performance was tested at monthly intervals up until rats were 26 months old. Two more groups were included: 26-month-old naive rats (Group 4); and 3- to 5-month-old naive rats (Group 5) both evaluated only once. At 26 months, the basal activity of ventral mesencephalic dopaminergic neurons was recorded. Results showed in Group 2 delay of motor detriments seen in aged rats, maintenance of basal firing rates of nigral cells compared to those of younger rats, and greater survival of substantia nigra cells. It is suggested that NGF cultured chromaffin cells produce a delay of motor detriments in aged rats, as a result of inducing survival and firing rates of nigral cells comparable to those seen in young rats.
Mendoza-Ramírez
J L
JL
Depto. de Neurociencias, Facultad de Medicina, Universidad Nacional Autónoma de México, D.F. México.
Beltrán-Parrazal
L
L
Verdugo-Díaz
L
L
Morgado-Valle
C
C
Drucker-Colín
R
R
D000367:Age Factors; D000375:Aging / Q000502:physiology*; D000818:Animals; D002478:Cells, Cultured; D002838:Chromaffin System / Q000378:metabolism*; D009043:Motor Activity / Q000502:physiology; D009414:Nerve Growth Factors / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D013378:Substantia Nigra / Q000378:metabolism
D009414:Nerve Growth Factors