title: Comparative studies of gamma-interferon receptor-like proteins of variola major and variola minor viruses.
abstract: To study specific properties of the human gamma-interferon (gamma-IFN) receptor-like proteins of the highly virulent and low virulent strains of variola (smallpox) virus (VAR) recombinant plasmids determining synthesis of these proteins in E. coli cells have been constructed. The recombinant viral gamma-IFN receptor-like proteins have been found to have high interferon-neutralising activity with regard to human gamma-IFN but not murine gamma-IFN and human alpha-IFN. The variola major and variola minor proteins under study do not differ in the efficiency of human gamma-IFN antiviral activity inhibition.
authors.affiliation: Institute of Molecular Biology, State Research Center of Virology and Biotechnology, Koltsovo, Russia.
authors.forename: S V
authors.initials: SV
authors.lastname: Seregin
authors.forename: I N
authors.initials: IN
authors.lastname: Babkina
authors.forename: A E
authors.initials: AE
authors.lastname: Nesterov
authors.forename: A N
authors.initials: AN
authors.lastname: Sinyakov
authors.forename: S N
authors.initials: SN
authors.lastname: Shchelkunov
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004926:Escherichia coli / Q000235:genetics; D005814:Genes, Viral / Q000235:genetics; D006801:Humans; D007371:Interferon-gamma / Q000037:antagonists & inhibitors*; D051379:Mice; D008969:Molecular Sequence Data; D017471:Receptors, Interferon / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000302:isolation & purification / Q000494:pharmacology; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D012901:Variola virus / Q000235:genetics / Q000472:pathogenicity*; D015678:Viral Structural Proteins / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology*; D014774:Virulence
chemical_list: D017471:Receptors, Interferon; D011993:Recombinant Fusion Proteins; D015678:Viral Structural Proteins; D007371:Interferon-gamma