The phzI gene of Pseudomonas aureofaciens 30-84 is responsible for the production of a diffusible signal required for phenazine antibiotic production.
The production of phenazine (Ph) antibiotics in Pseudomonas aureofaciens (Pau) 30-84 is positively regulated by PhzR, a protein belonging to the LuxR family of transcriptional activators. We have now identified phzI, a second gene required for PH production. The product of phzI is a member of the LuxI family of N-acyl-homoserine lactone (N-acyl-HSL) synthases. Inactivation of phzI results in the loss of Ph production in Pau 30-84. The presence of phzI in Escherichia coli is sufficient for the production of a diffusible signal which activates phzB expression in Pau 30-84 and traA expression in a N-acyl-HSL-dependent reporter strain of Agrobacterium tumefaciens. In addition, synthetic N-(3-oxohexanoyl)-L-HSL induces phzB expression in Pau 30-84. These results suggest that Pau 30-84 produces a N-acyl-HSL signal that regulates Ph production, and that phzI plays a central role in this signaling pathway.
Wood
D W
DW
Department of Plant Pathology, University of Arizona, Tucson, 85721, USA.
Pierson
L S
LS
D015107:4-Butyrolactone / Q000031:analogs & derivatives / Q000378:metabolism / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000494:pharmacology; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D001483:Base Sequence; D002455:Cell Division / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression / Q000235:genetics; D005798:Genes, Bacterial; D005809:Genes, Regulator; D008969:Molecular Sequence Data; D010619:Phenazines / Q000378:metabolism* / Q000494:pharmacology; D010675:Pheromones / Q000494:pharmacology; D011401:Promoter Regions, Genetic / Q000235:genetics; D011549:Pseudomonas / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics; D015183:Restriction Mapping; D013053:Spectrophotometry; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; C085427:LuxI protein, Bacteria; D010619:Phenazines; D010675:Pheromones; C098602:PhzI protein, Pseudomonas sp.; C088011:PhzR protein, Pseudomonas; D011993:Recombinant Fusion Proteins; D015534:Trans-Activators; D014157:Transcription Factors; C088386:homoserine lactone; C030654:N-(3-oxohexanoyl)-3-aminodihydro-2(3H)-furanone; D001616:beta-Galactosidase; D015107:4-Butyrolactone