title: Neutralization of human cytomegalovirus by human CD13-specific antibodies.
abstract: The mechanisms of inhibition of cytomegalovirus (CMV) infection by human CD13 (aminopeptidase N)-specific antibodies were studied. These antibodies protect CD13-negative and -positive cells from CMV infection only if incubated with the virus inoculum, suggesting they bind to CMV virions. The association of a CD13-like molecule with virions was further supported by the transfer of CD13 immunoreactivity to the surface of CD13-negative cells upon binding of CMV; the binding of CD13-specific antibodies directly to the surface of CMV virions; and the presence of anti-CD13 immunoreactive bands, including one with mobility similar to that of native cellular CD13 on immunoblots of proteins of purified CMV particles. Importantly, CD13-specific antibodies neutralize CMV in urine of neonates with congenital CMV, indicating that the CD13-like molecule is associated with natural CMV and not acquired in vitro. These studies demonstrate that a CD13-like molecule is associated with CMV particles and may be important in CMV pathogenesis.
authors.affiliation: Division of Pediatrics, City of Hope National Medical Center, Duarte, California 91010-0269, USA.
authors.forename: T D
authors.initials: TD
authors.lastname: Giugni
authors.forename: C
authors.initials: C
authors.lastname: Söderberg
authors.forename: D J
authors.initials: DJ
authors.lastname: Ham
authors.forename: R M
authors.initials: RM
authors.lastname: Bautista
authors.forename: K O
authors.initials: KO
authors.lastname: Hedlund
authors.forename: E
authors.initials: E
authors.lastname: Möller
authors.forename: J A
authors.initials: JA
authors.lastname: Zaia
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000914:Antibodies, Viral / Q000276:immunology*; D000918:Antibody Specificity; D018826:CD13 Antigens / Q000276:immunology*; D002460:Cell Line; D003429:Cross Reactions; D003587:Cytomegalovirus / Q000276:immunology*; D003586:Cytomegalovirus Infections / Q000151:congenital / Q000276:immunology / Q000821:virology; D000939:Epitopes / Q000032:analysis; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D051379:Mice; D009500:Neutralization Tests; D014771:Virion / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000939:Epitopes; D018826:CD13 Antigens