Increased oxidative DNA damage in Helicobacter pylori-infected human gastric mucosa.
Helicobacter pylori causes type B gastritis. It shows strong association with the development of gastric carcinoma. A plausible hypothesis for the missing link between H. pylori infection and gastric carcinogenesis involves oxygen free radical-induced DNA damage. To test this hypothesis, we compared the amount of 9-hydroxydeoxyguanosine, a marker for oxygen free radical-induced DNA damage, in the DNA of human gastric mucosa with and without H. pylori infection. Gastric antral biopsies were taken from pediatric patients and volunteers to select H. pylori-positive and H. pylori-negative specimens. The 8-hydroxydeoxyguanosine content of the gastric mucosal DNA was measured after H. pylori-positive and H. pylori-negative volunteers were identified. The increased level of oxidative DNA damage suggests the mechanistic link between H. pylori infection and gastric carcinoma.
Baik
S C
SC
Department of Microbiology, Gyeongsang National University College of Medicine, Chinju, Kyung-Nam, Republic of Korea.
Youn
H S
HS
Chung
M H
MH
Lee
W K
WK
Cho
M J
MJ
Ko
G H
GH
Park
C K
CK
Kasai
H
H
Rhee
K H
KH
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D004247:DNA / Q000737:chemistry* / Q000187:drug effects; D004249:DNA Damage; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000032:analysis; D005260:Female; D005753:Gastric Mucosa / Q000737:chemistry* / Q000382:microbiology / Q000473:pathology; D005756:Gastritis / Q000235:genetics* / Q000382:microbiology / Q000473:pathology; D016481:Helicobacter Infections / Q000150:complications* / Q000235:genetics / Q000473:pathology; D016480:Helicobacter pylori; D006801:Humans; D007223:Infant; D008297:Male; D017382:Reactive Oxygen Species / Q000633:toxicity*
D017382:Reactive Oxygen Species; D000080242:8-Hydroxy-2'-Deoxyguanosine; D004247:DNA; D003849:Deoxyguanosine