title: Production and crystallization of MHC class I B allele single peptide complexes.
abstract: Major histocompatibility complex class I B alleles, HLA B8, B53 and B3501 have been cloned, expressed, refolded and crystallized in specific complexes with a number of different 8-mer and 9-mer peptides. For some of these crystallization was initiated by cross-seeding between different B allele complexes. All crystallize in the space group P212121, with similar unit cell dimensions of approximately 52 A X 81 A X 112 A, contain one complex per asymmetric unit and diffract to approximately 2.0 A resolution.
authors.affiliation: Laboratory of Molecular Biophysics, Oxford, UK.
authors.forename: S W
authors.initials: SW
authors.lastname: Reid
authors.forename: K J
authors.initials: KJ
authors.lastname: Smith
authors.forename: B K
authors.initials: BK
authors.lastname: Jakobsen
authors.forename: C A
authors.initials: CA
authors.lastname: O'Callaghan
authors.forename: H
authors.initials: H
authors.lastname: Reyburn
authors.forename: K
authors.initials: K
authors.lastname: Harlos
authors.forename: D I
authors.initials: DI
authors.lastname: Stuart
authors.forename: A J
authors.initials: AJ
authors.lastname: McMichael
authors.forename: J I
authors.initials: JI
authors.lastname: Bell
authors.forename: E Y
authors.initials: EY
authors.lastname: Jones
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D017931:DNA Primers; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000737:chemistry; D004926:Escherichia coli / Q000235:genetics; D005805:Genes, MHC Class I / Q000235:genetics*; D006680:HLA Antigens / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D015795:HLA-B8 Antigen / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000737:chemistry / Q000378:metabolism*; D011487:Protein Conformation; D017510:Protein Folding; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D017931:DNA Primers; D000939:Epitopes; D006680:HLA Antigens; C078438:HLA-B53 antigen; D015795:HLA-B8 Antigen; D009842:Oligopeptides; D011994:Recombinant Proteins