Effects of degraded schizophyllans on regeneration of protoplast cells of Saccharomyces cerevisiae.
Schizophyllan was heated at 100 degrees C in 85% dimethyl sulfoxide (DMSO) containing 0.01 M H2SO4 for various times, and fractionated by gel-permeation chromatography. Molecular weights (M(r)) of the depolymerized products thus obtained were measured in water and DMSO by GPC-LALLS to estimate their conformations in water. The products with triple helical structure stimulated regeneration of Saccharomyces cerevisiae protoplast cells, while those of single chain conformation were totally inactive.
Hisamatsu
M
M
Department of Agricultural Chemistry, Faculty of Bioresources, Mie University, Tsu, Japan.
Hayano
M
M
Mishima
T
T
Teranishi
K
K
Yamada
T
T
D006868:Hydrolysis; D011523:Protoplasts / Q000187:drug effects* / Q000502:physiology*; D012038:Regeneration / Q000187:drug effects*; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology*; D012566:Sizofiran / Q000378:metabolism* / Q000494:pharmacology*
D012566:Sizofiran