Improved single-step PCR assay for sex identification post-allogeneic sex-mismatched BMT.
A method is presented which allows sex-identification simultaneously of both male and female cells in cell mixtures. The sensitivity of this PCR-based assay is one male cell in 10(6) female cells. The test can be used routinely to analyze bone marrow and peripheral blood lymphocytes post-allogeneic sex-mismatched bone marrow transplantation to detect early engraftment or rejection, as well as host cell regeneration.
Pugatsch
T
T
Department of Bone Marrow Transplantation, Hadassah University Hospital, Jerusalem, Israel.
Oppenheim
A
A
Slavin
S
S
D053523:Amelogenin; D001483:Base Sequence; D001853:Bone Marrow / Q000473:pathology; D016026:Bone Marrow Transplantation / Q000473:pathology*; D002470:Cell Survival; D002678:Chimera / Q000235:genetics*; D017931:DNA Primers; D003746:Dental Enamel Proteins / Q000235:genetics; D005260:Female; D005819:Genetic Markers; D006085:Graft Survival / Q000235:genetics*; D006801:Humans; D008214:Lymphocytes / Q000648:ultrastructure; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D012732:Sex Determination Analysis / Q000379:methods*; D014960:X Chromosome / Q000235:genetics; D014998:Y Chromosome / Q000235:genetics
D053523:Amelogenin; D017931:DNA Primers; D003746:Dental Enamel Proteins; D005819:Genetic Markers