title: Modulation of vitellogenin II gene by estradiol and progesterone in the Japanese quail.
abstract: Estrogen and progesterone receptors are reported to functionally cooperate in gene activation if their cognate binding sites are close to one another in the gene. Our studies show that the expression of the vitellogenin (VTG) gene is induced by estradiol alone or along with progesterone. Progesterone alone inhibits the expression completely. Methylation status of the VTG gene remains unaltered by steroid hormones. Gel mobility shift assay shows that qualitative and quantitative changes occur in the trans-acting factor(s) that bind to estradiol and progesterone responsive elements (ERE and PRE) after administration of these steroid hormones. We, therefore, conclude that the interaction of trans-acting factors that bind to ERE and PRE play a role in the regulation of VTG gene expression.
authors.affiliation: Department of Zoology, Baharas Hindu University, Varanasi, India.
authors.forename: S
authors.initials: S
authors.lastname: Gupta
authors.forename: M S
authors.initials: MS
authors.lastname: Kanungo
mesh_terms: D000818:Animals; D001665:Binding Sites; D003370:Coturnix; D004268:DNA-Binding Proteins / Q000378:metabolism; D004958:Estradiol / Q000502:physiology*; D005786:Gene Expression Regulation; D008745:Methylation; D011374:Progesterone / Q000502:physiology*; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics; D015533:Transcriptional Activation; D014819:Vitellogenins / Q000235:genetics*
chemical_list: D004268:DNA-Binding Proteins; D012333:RNA, Messenger; D014819:Vitellogenins; D011374:Progesterone; D004958:Estradiol