Polymorphism of F-actin assembly. 1. A quantitative phase diagram of F-actin.
We have made the first quantitative phase diagram of actin filament (F-actin) assembly represented by the concentration of F-actin and the chi parameter which characterizes solvent-solute interaction energy. We manipulated the chi value of F-actin by adding a high molecular weight poly- (ethylene glycol) with average molecular weight 6000 (PEG 6K). The preferential exclusion of PEG 6K from the region adjacent to F-actin increases the chi value of F-actin. We qualified the PEG 6K-induced increase of the chi value through analysis of the PEG-induced solubility change of protein. The phase diagram shows that F-actin changes its assembly structure from isotropic disordered distribution to anisotropic ordered phase of a lyotropic liquid crystalline with an increase in the concentration and to concentrated anisotropic ordered phase of a crystalline-like bundle with a small increase in chi respectively, in the physiological concentration range. The formation of the crystalline- like bundle suggests that some specific force may act between F-actin. The present results demonstrate that F-actin can take various assembly structures as observed in cytoplasm by itself, indicating that the versatility of F-actin assembly in cytoplasm may be based on the thermodynamic properties of F-actin as a rod-like molecule.
Suzuki
A
A
Department of Biophysics, Kyoto University School of Science, Japan.
Yamazaki
M
M
Ito
T
T
D000199:Actins / Q000737:chemistry* / Q000187:drug effects / Q000648:ultrastructure; D003460:Crystallization; D008956:Models, Chemical; D011092:Polyethylene Glycols / Q000494:pharmacology; D012995:Solubility; D013816:Thermodynamics
D000199:Actins; D011092:Polyethylene Glycols