Electrospray-collision-induced dissociation mass spectrometry of mono-, di- and tri-hydroxylated lipoxygenase products, including leukotrienes of the B-series and lipoxins.
Collision-induced dissociation (CID) mass spectra were recorded on underivatized, hydroxylated lipoxygenase products, including S(S)-, 12(S)- and 15(S)-hydroxyeicosatetraenoic adds (HETE), leukotriene (LT) B4, LTB5, the LTB4 metabolites 20-OH-LTB4, 20-COOH-LTB4 and 10,11-dihydro-LTB4, the LTB4 isomers 6-trans-LTB4, 6-trans,12-epi-LTB4 and S(S),12(S)-dihydroxyeicosatetraenoic acid (diHETE) as well as S(S),6(R)-diHETE, 14(S), 15(R)-diHETE and lipoxin (LX) A4 and B4. Pseudomolecular [M-H]- parent ions were generated by electrospray ionization. The ions were accelerated to 4 keV, selected by the double focusing sectors Of 2 hybrid tandem mass spectrometer, decelerated to 400 eV and focused into a collision cell containing xenon. The resulting fragment ions were mass analysed by an orthogonal time-of-flight analyser. The CID spectra obtained were structurally informative allowing isomer differentiation.
Griffiths
W J
WJ
Division of Physiological Chemistry, Department of Medical Biochemistry & Biophysics, Karolinska Institutet, Stockholm, Sweden.
Yang
Y
Y
Sjövall
J
J
Lindgren
J A
JA
D001792:Blood Platelets / Q000737:chemistry / Q000187:drug effects; D000001:Calcimycin / Q000494:pharmacology; D015777:Eicosanoids / Q000097:blood / Q000378:metabolism*; D006098:Granulocytes / Q000737:chemistry; D006801:Humans; D006893:Hydroxyeicosatetraenoic Acids / Q000097:blood / Q000378:metabolism; D066298:In Vitro Techniques; D015289:Leukotrienes / Q000097:blood / Q000378:metabolism*; D008084:Lipoxygenase / Q000097:blood / Q000378:metabolism*; D013058:Mass Spectrometry
D015777:Eicosanoids; D006893:Hydroxyeicosatetraenoic Acids; D015289:Leukotrienes; D000001:Calcimycin; D008084:Lipoxygenase