Toward cell-targeting gene therapy vectors: selection of cell-binding peptides from random peptide-presenting phage libraries.
Ideal gene therapy vectors would be delivered intravenously to transfect only specific cells. Existing vectors only transfect cells in vivo in a manner determined by blood flow and the site of introduction. As a general and systematic approach for generating cell-targeting ligands for gene therapy vectors, we have used peptide-presenting phage libraries to select peptides that bind and enter several different cell types. Because of their small size, cell-binding peptides such as these could be incorporated into biological or physical gene therapy vectors. In addition, peptide-presenting phage themselves may also be candidates for gene therapy vectors.
Barry
M A
MA
Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, 75235-8573, USA.
Dower
W J
WJ
Johnston
S A
SA
D000595:Amino Acid Sequence; D000818:Animals; D001435:Bacteriophages / Q000235:genetics; D001667:Binding, Competitive; D002460:Cell Line; D005347:Fibroblasts / Q000378:metabolism; D015723:Gene Library; D018390:Gene Targeting / Q000379:methods*; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors; D007150:Immunohistochemistry; D051379:Mice; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009154:Mutation; D010455:Peptides / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D011485:Protein Binding; D011487:Protein Conformation
D010455:Peptides