Genomic structure of carp mitogen-activated protein kinase kinase 1 gene.
Carp mitogen-activated protein kinase kinase 1 (cMKK1) gene was isolated from a liver genomic library. The sequence around the exon-intron boundaries and 2 kb of the promoter region were determined. Our data indicate that this gene is composed of 11 exons and 10 introns spanning about 9 kb. Multiple potential transcription initiation sites were located by primer extension analysis. Examination of 2 kb of 5'-flanking sequence revealed potential binding sites for a variety of transcription factors such as E2F, Ets-1, GATA-1, Myb, NF-IL6, Sp1, and NF-kB.
Leu
J H
JH
Institute of Biological Chemistry, Academia Sinicia, Taipei, Taiwan.
Lee
M S
MS
Chen
K T
KT
Chang
G D
GD
Chou
C k
Ck
Huang
C J
CJ
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002347:Carps / Q000235:genetics*; D002352:Carrier Proteins; D018797:Cell Cycle Proteins; D018387:Codon, Initiator; D018076:DNA, Complementary; D004268:DNA-Binding Proteins / Q000378:metabolism; D050684:E2F Transcription Factors; D050981:Erythroid-Specific DNA-Binding Factors; D048369:MAP Kinase Kinase 1; D020929:Mitogen-Activated Protein Kinase Kinases; D008969:Molecular Sequence Data; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*; D011505:Protein-Tyrosine Kinases / Q000235:genetics*; D056465:Retinoblastoma-Binding Protein 1; D016329:Sp1 Transcription Factor / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism; D014158:Transcription, Genetic
D002352:Carrier Proteins; D018797:Cell Cycle Proteins; D018387:Codon, Initiator; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D050684:E2F Transcription Factors; D050981:Erythroid-Specific DNA-Binding Factors; D056465:Retinoblastoma-Binding Protein 1; D016329:Sp1 Transcription Factor; D014157:Transcription Factors; D011505:Protein-Tyrosine Kinases; D017346:Protein Serine-Threonine Kinases; D048369:MAP Kinase Kinase 1; D020929:Mitogen-Activated Protein Kinase Kinases