Detection of BCR/ABL fusion gene in CML: a preliminary report.
The BCR/ABL fusion gene in 31 patients with chronic myeloid leukemia (CML) was detected by RT/PCR. In 18 cases of Ph' positive patients, 13 had BCR 3/ABL II rearrangement, 1 had BCR 2/ABL II rearrangement and 4 had both rearrangements. One case with complex translocation: 46,XY,t(9;9;22), had BCR 3/ABL II rearrangement. In 8 cases of Ph' negative patients, 4 had BCR 3/ABL II rearrangement, 3 had both rearrangements while 1 had no BCR/ABL rearrangement. Interestingly, in 4 patients who had no cytogenetic result, we could observe BCR 3/ABL II rearrangement in 3 cases and both rearrangements in 1 case. The results suggest that this procedure is sensitive and independent of the presence or absence of an identifiable Ph' chromosome.
Rerkamnuaychoke
B
B
Department of Pathology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Thailand.
Kangwanpong
D
D
Jootar
S
S
Puranakanitstha
C
C
D001853:Bone Marrow / Q000473:pathology; D005091:Exons; D005260:Female; D016044:Fusion Proteins, bcr-abl / Q000096:biosynthesis / Q000235:genetics*; D015321:Gene Rearrangement; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000097:blood / Q000235:genetics* / Q000473:pathology; D008297:Male; D010677:Philadelphia Chromosome; D016133:Polymerase Chain Reaction; D014158:Transcription, Genetic
D016044:Fusion Proteins, bcr-abl