Paramagnetic cobalt and nickel derivatives of Alcaligenes denitrificans azurin and its M121Q mutant. A 1H NMR study.
Using cobalt or nickel to replace copper in native azurin allows one to fingerprint the metal coordination site of the protein. The metal sites of wild type Alcaligenes denitrificans azurin and its M121Q mutant are clearly distinguishable through the paramagnetic 1H NMR spectra of the Ni(II) and Co(II) derivatives. In the wild type azurin, Gly45 coordinates to nickel or cobalt, while Met121 appears as a weak metal ligand. On the contrary, in the M121Q azurin mutant, the metal exhibits a clear preference for the Gln121, which coordinates through the side chain carbonyl oxygen, and Gly45 is not a ligand. Changes in the isotropic shifts and relaxation properties of signals from the Cys112, His46, and His117 metal ligands suggest a movement of the metal ion out of the equatorial plane, indicating that the metal site is tetrahedral. These effects are less pronounced in the Ni(II) M121Q azurin than in the Co(II) metalloderivative. The similarity between the NMR spectra of the Co(II) derivatives of stellacyanin and the M121Q azurin is in agreement with a very similar metal site in both proteins and supports the existence of a coordinated Gln in stellacyanin.
Salgado
J
J
Department of Inorganic Chemistry, University of Valencia, Burjassot, Spain.
Jiménez
H R
HR
Moratal
J M
JM
Kroes
S
S
Warmerdam
G C
GC
Canters
G W
GW
D000421:Alcaligenes / Q000235:genetics / Q000378:metabolism; D001400:Azurin / Q000031:analogs & derivatives* / Q000737:chemistry* / Q000235:genetics; D001665:Binding Sites; D003035:Cobalt / Q000737:chemistry; D004579:Electron Transport; D009682:Magnetic Resonance Spectroscopy; D008667:Metalloproteins / Q000737:chemistry; D015394:Molecular Structure; D009532:Nickel / Q000737:chemistry; D010940:Plant Proteins / Q000737:chemistry; D017354:Point Mutation; D011487:Protein Conformation; D013053:Spectrophotometry; D013056:Spectrophotometry, Ultraviolet
D008667:Metalloproteins; D010940:Plant Proteins; D001400:Azurin; C003343:stellacyanin protein, plant; D003035:Cobalt; D009532:Nickel