title: Effects of neocarzinostatin-chimeric Fab conjugates on the growth of human pancreatic carcinoma xenografts.
abstract: Neocarzinostatin (NCS) was bound covalently to human/mouse chimeric Fab fragments of MAb A7 (chA7Fab) directed against human pancreatic carcinoma. The anti-tumour effect of chA7Fab-NCS was tested in a nude mouse model on pancreatic carcinoma and compared with A7-NCS or NCS alone. The anti-tumour effect of chA7Fab-NCS increased in a dose-dependent manner and was significantly greater than either A7-NCS or NCS. Tumour growth was completely suppressed after the administration of chA7Fab-NCS. An enzyme-linked immunosorbent assay with rabbit anti-mouse immunoglobulin was performed to examine the antigenicity of chA7Fab. ChA7Fab had less reactivity with rabbit anti-mouse immunoglobulin than either whole antibody A7 or murine Fab fragments of A7. Thus, chA7Fab-NCS can inhibit human pancreatic cancer growth in an animal and may be useful for targeting chemotherapy to pancreatic cancer in humans.
authors.affiliation: First Department of Surgery, Kyoto Prefectural University of Medicine, Japan.
authors.forename: E
authors.initials: E
authors.lastname: Otsuji
authors.forename: T
authors.initials: T
authors.lastname: Yamaguchi
authors.forename: H
authors.initials: H
authors.lastname: Tsuruta
authors.forename: Y
authors.initials: Y
authors.lastname: Yata
authors.forename: H
authors.initials: H
authors.lastname: Nishi
authors.forename: K
authors.initials: K
authors.lastname: Okamoto
authors.forename: K
authors.initials: K
authors.lastname: Taniguchi
authors.forename: M
authors.initials: M
authors.lastname: Kato
authors.forename: T
authors.initials: T
authors.lastname: Kotani
authors.forename: K
authors.initials: K
authors.lastname: Kitamura
authors.forename: T
authors.initials: T
authors.lastname: Takahashi
mesh_terms: D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000096:biosynthesis; D002294:Carcinoma, Squamous Cell / Q000628:therapy*; D006801:Humans; D000922:Immunotoxins / Q000008:administration & dosage*; D051379:Mice; D008819:Mice, Nude; D009374:Neoplasms, Experimental; D010190:Pancreatic Neoplasms / Q000628:therapy*; D011993:Recombinant Fusion Proteins; D014183:Transplantation, Heterologous; D009353:Zinostatin / Q000008:administration & dosage*
chemical_list: D000888:Antibodies, Anti-Idiotypic; D000922:Immunotoxins; D011993:Recombinant Fusion Proteins; D009353:Zinostatin