Reduced carnitine palmitoyl transferase activity and altered acyl-trafficking in red blood cells from hemodialysis patients.
We measured carnitine palmitoyl transferase activity, free carnitine, and long chain acyl carnitine levels in erythrocytes from 15 uremic patients and 25 controls. Carnitine palmitoyl transferase levels in patients were significantly lower than in controls. The levels of free carnitine and long chain acyl carnitines as well as the long chain acyl carnitine/free carnitine ratio were significantly higher in patients than in controls. Our results suggest that hemodialysis causes alteration in the acyl-trafficking in red blood cells membrane.
de los Reyes
B
B
Centro de Investigacíon, Hosp. 12 de Octubre, Madrid, Spain.
Perez-García
R
R
Liras
A
A
Arenas
J
J
D000328:Adult; D000368:Aged; D002331:Carnitine / Q000097:blood*; D002334:Carnitine O-Palmitoyltransferase / Q000097:blood*; D003576:Electron Transport Complex IV / Q000097:blood; D004910:Erythrocyte Membrane / Q000201:enzymology / Q000378:metabolism; D004912:Erythrocytes / Q000201:enzymology / Q000378:metabolism*; D005260:Female; D006801:Humans; D008297:Male; D008563:Membrane Lipids / Q000097:blood; D008565:Membrane Proteins; D008875:Middle Aged; D010743:Phospholipids / Q000097:blood; D006435:Renal Dialysis; D014511:Uremia / Q000097:blood*
D008563:Membrane Lipids; D008565:Membrane Proteins; D010743:Phospholipids; D003576:Electron Transport Complex IV; D002334:Carnitine O-Palmitoyltransferase; D002331:Carnitine