A delayed-early response nuclear gene encoding MRPL12, the mitochondrial homologue to the bacterial translational regulator L7/L12 protein.
We have characterized a new delayed-early response mRNA encoding a 21-kDa product (MRPL12) that accumulates during the G1 phase of growth-stimulated cells. MRPL12 is the mammalian homologue to chloroplastic and bacterial L12 ribosomal proteins. Immunofluorescence microscopy and cell fractionation indicate a predominant mitochondrial localization in various mammalian cell lines. The NH2-terminal 49 amino acids are necessary and sufficient to target the protein within the mitochondria and are probably cleaved off during import. MRPL12 proteins associated in vitro and cofractionate with ribosomal structures, as is the case for prokaryotic L12 proteins. Expression of a dominant inhibitory truncated protein leads to a severe reduction in cell growth by inhibiting mitochondrial ATP production. MRPL12 is the first mammalian mitochondrial ribosomal protein to be characterized.
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Institut de Génétique Moléculaire, UMR5535, CNRS-Universités Montpellier I et II, France.
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D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins / Q000378:metabolism; D001483:Base Sequence; D018797:Cell Cycle Proteins; D002455:Cell Division; D002460:Cell Line; D002467:Cell Nucleus; D002522:Chlorocebus aethiops; D002736:Chloroplasts / Q000378:metabolism; D003001:Cloning, Molecular; D006224:Cricetinae; D018076:DNA, Complementary; D004926:Escherichia coli / Q000378:metabolism; D015870:Gene Expression; D001411:Geobacillus stearothermophilus / Q000378:metabolism; D005982:Glutathione Transferase / Q000096:biosynthesis; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D008928:Mitochondria / Q000378:metabolism*; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000096:biosynthesis* / Q000235:genetics* / Q000378:metabolism; D009838:Oligodeoxyribonucleotides; D010802:Phylogeny; D014176:Protein Biosynthesis; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000378:metabolism; D012269:Ribosomal Proteins / Q000096:biosynthesis* / Q000235:genetics* / Q000378:metabolism*; D012270:Ribosomes; D017386:Sequence Homology, Amino Acid; D018724:Spinacia oleracea
D001426:Bacterial Proteins; D018797:Cell Cycle Proteins; D018076:DNA, Complementary; C099418:MRPL12 protein, human; D009687:Nuclear Proteins; D009838:Oligodeoxyribonucleotides; D011993:Recombinant Fusion Proteins; D012269:Ribosomal Proteins; C016700:ribosomal protein L7-L12; D005982:Glutathione Transferase