Genomic rearrangement in genome segment 12 of rice dwarf phytoreovirus.
The genome segment 12 (S12) of rice dwarf phytoreovirus (RDV) isolated from the Philippines (RDV-P) and of a variant (RDV-S-6) of RDV severe strain (RDV-S) migrated abnormally slower during polyacrylamide gel electrophoresis than that of the isolate maintained at Hokkaido University (RDV-H). Nucleotide sequence analysis revealed that rearrangement had occurred in these segments, affecting the open reading frame. A polypeptide encoded by S12 (Pns12) of RDV-P had a duplication of 28 amino acids while 1/3 of the carboxyl terminus of Pns12 was deleted in RDV-S-6 by premature termination due to a frameshift. RDV-S is always present in plants infected with the RDV-S-6 variant, suggesting that Pns12 of RDV-S-6 is defective. On the other hand, Pns12 of RDV-P was expressed and appeared to be functional in infected cells in spite of the duplication, as demonstrated by immunoblot analyses using antibody raised against Pns12 expressed in Escherichia coli.
Murao
K
K
Department of Agrobiology and Bioresources, Faculty of Agriculture, Hokkaido University, Sapporo, Japan.
Uyeda
I
I
Ando
Y
Y
Kimura
I
I
Cabauatan
P Q
PQ
Koganezawa
H
H
D001483:Base Sequence; D017931:DNA Primers; D015321:Gene Rearrangement; D016679:Genome, Viral; D008969:Molecular Sequence Data; D012275:Oryza / Q000821:virology; D012367:RNA, Viral / Q000235:genetics; D012087:Reoviridae / Q000235:genetics*
D017931:DNA Primers; D012367:RNA, Viral