Evaluation of the Abbott Cell-DYN 3500 hematology analyzer in university hospital.
The Abbott (R) Cell-Dyn 3500 (Abbott CD 3500, Abbott Diagnostics Division, Mountain View, CA) is a fully automated hematology analyzer capable of providing a complete blood count (CBC) profile, including a five-part differential leukocyte count (DLC) and flagging system in this study. The CBC profile and DLC flagging system of the Abbott CD 3500 were evaluated according to the HA-20 protocol of the National Committee for Clinical and Laboratory Standards (NCCLS) and compared to the Technicon H*2 blood analyzer currently used in the authors' laboratory. Linearity, carryover, precision, and stability were all within the limits established by the manufacturer. No significant break-downs were found during the evaluation period. Evaluation of DLC indicated an excellent correlation with the manual reference method for neutrophils, lymphocytes, and eosinophils (r = 0.916, 0.936, 0.967, respectively), a good correlation for monocytes (r = 0.811) and a poor correlation for basophils (r = 0.224). Overall flagging for morphologic abnormalities displayed higher sensitivity (85%) than specificity (67%), with a false-positive ratio of 33%. In general, these results are in accordance with those obtained by other authors in the same period of time.
Vives-Corrons
J L
JL
Hematology Laboratory Department, Hospital Clínic i Provincial, University of Barcelona, Spain.
Besson
I
I
Jou
J M
JM
Gutierrez
G
G
D000046:Academic Medical Centers; D001772:Blood Cell Count / Q000295:instrumentation* / Q000379:methods; D001793:Blood Preservation; D001800:Blood Specimen Collection; D003951:Diagnostic Errors; D005069:Evaluation Studies as Topic; D006801:Humans; D007958:Leukocyte Count / Q000379:methods; D015203:Reproducibility of Results; D013696:Temperature; D013997:Time Factors