Identification and synthetic pathway of sialyl-Lewis(x)-containing neolacto-series gangliosides in lens tissues. 2. Enzymatic synthesis of sialyl-Lewis(x) gangliosides in monkey and rat lenses.
In Japanese monkey lenses, 3H-labeled fucose and N-acetylneuraminic acid were enzymatically transferred to neolactotetraosylceramide (nLc4) and III 3 FucnLc4, respectively, suggesting the presence of a synthetic pathway of IV3 NeuAcIII3 FucnLc4 via III3 FucnLc4 in monkey lenses. Six rat strains, Wistar, Sprague-Dawley and pigmented strains, contained sialyl-Lewis(x) gangliosides in non-cataractous lenses in a strain-specific manner. Glycosyltransferase assay revealed that the transfer of 3H-labeled fucose to nLc4 occurred in all the strains, but that the transfer of 3H-labeled N-acetylneuraminic acid to III3 FucnLc4 was strain-specific. These results suggested that sialyl-Lewis(x) gangliosides were generally synthesized from neolactotetraosylceramide via Lewis(x) glycolipid (III3 FucnLc4) in lens tissues, differing from other tissues. Combining our results, we propose two synthetic pathways of sialyl-Le(x)- containing neolacto-series gangliosides and A-pathway ganglio-series gangliosides in human senile cataractous lens: one to sialyl-Lewis(x) gangliosides from nLc4 via Lewis(x) glycolipid, and the other to GD1a from GM3, via GM2 and GM1.
Ogiso
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Cell and Information, PRESTO, Research Development Corporation of Japan (JRDC), Tokyo, Japan.
Komoto
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Hoshi
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D000818:Animals; D002240:Carbohydrate Sequence; D002855:Chromatography, Thin Layer; D003569:Cytidine Monophosphate N-Acetylneuraminic Acid / Q000378:metabolism; D005643:Fucose / Q000378:metabolism; D005732:Gangliosides / Q000096:biosynthesis*; D005915:Globosides / Q000378:metabolism; D006028:Glycosphingolipids / Q000096:biosynthesis* / Q000737:chemistry; D016695:Glycosyltransferases / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D015151:Immunoblotting; D007908:Lens, Crystalline / Q000378:metabolism*; D007983:Lewis Blood Group Antigens; D016256:Lewis X Antigen / Q000378:metabolism; D008251:Macaca; D008969:Molecular Sequence Data; D051381:Rats; D011919:Rats, Inbred Strains; D000080506:Sialyl Lewis X Antigen; D005644:alpha-L-Fucosidase / Q000378:metabolism
D005732:Gangliosides; D005915:Globosides; D006028:Glycosphingolipids; D007983:Lewis Blood Group Antigens; D016256:Lewis X Antigen; D000080506:Sialyl Lewis X Antigen; C094688:sialyl Lewis(x) ganglioside; D005643:Fucose; D003569:Cytidine Monophosphate N-Acetylneuraminic Acid; C036363:paragloboside; D016695:Glycosyltransferases; D005644:alpha-L-Fucosidase