Quantitative competitive polymerase chain reaction: analysis of amplified products of the HIV-1 gag gene by capillary electrophoresis with laser-induced fluorescence detection.
A quantitative competitive polymerase chain reaction (PCR) assay for the detection and quantification of HIV-1 has been developed using capillary electrophoresis. Separation of the PCR products is carried out in coated capillaries filled with replaceable linear polyacrylamide, and detection is performed using laser-induced fluorescence. The quantitative capabilities of this assay are described. We show results from analysis of a noninfectious plasmid encoding the HIV genome and integrated proviral DNA. Potential applications of this assay are discussed.
Williams
S J
SJ
Department of Analytical Chemistry, Genentech, Inc., 460 Point San Bruno Boulevard, South San Francisco, California, 94080, USA.
Schwer
C
C
Krishnarao
A S
AS
Heid
C
C
Karger
B L
BL
Williams
P M
PM
D002460:Cell Line; D004279:DNA, Viral / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000379:methods*; D015750:Genes, gag; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D011533:Proviruses / Q000737:chemistry / Q000235:genetics; D013050:Spectrometry, Fluorescence
D004279:DNA, Viral