title: Antitumor effect induced by the expression of granulocyte macrophage-colony stimulating factor gene in murine colon carcinoma cells.
abstract: Murine colon carcinoma cells which secrete several kinds of cytokine after retroviral transduction with corresponding genes, were examined for their antitumor effects in syngeneic mice. The mice inoculated with granulocyte macrophage-colony stimulating factor (GM-CSF) producer cells showed not only prolonged survival but also reduced tumorigenicity. The antitumor effect caused by the expression of interleukin-4 was less than that of GM-CSF, and interleukin-6 producer cells did not show any effects on the survival of the host animals. Histological examination of the GM-CSF-producing tumor revealed predominant infiltration of neutrophils and necrotic change of the tumor. The present study indicates the feasibility of cancer gene therapy with the expression of GM-CSF gene in tumor cells.
authors.affiliation: Department of Surgery (II), School of Medicine, Chiba University, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Gunji
authors.forename: M
authors.initials: M
authors.lastname: Tagawa
authors.forename: H
authors.initials: H
authors.lastname: Matsubara
authors.forename: K
authors.initials: K
authors.lastname: Takenaga
authors.forename: H
authors.initials: H
authors.lastname: Shimada
authors.forename: F
authors.initials: F
authors.lastname: Kondo
authors.forename: T
authors.initials: T
authors.lastname: Suzuki
authors.forename: K
authors.initials: K
authors.lastname: Nakajima
authors.forename: M
authors.initials: M
authors.lastname: Sugaya
authors.forename: T
authors.initials: T
authors.lastname: Asano
authors.forename: T
authors.initials: T
authors.lastname: Ochiai
authors.forename: K
authors.initials: K
authors.lastname: Isono
authors.forename: K
authors.initials: K
authors.lastname: Horitsu
authors.forename: H
authors.initials: H
authors.lastname: Kageyama
authors.forename: Y
authors.initials: Y
authors.lastname: Nakamura
authors.forename: S
authors.initials: S
authors.lastname: Sakiyama
mesh_terms: D000818:Animals; D003110:Colonic Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D005240:Feasibility Studies; D005260:Female; D015870:Gene Expression; D015316:Genetic Therapy; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000235:genetics* / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D014162:Transfection; D014407:Tumor Cells, Cultured
chemical_list: D015850:Interleukin-6; D015847:Interleukin-4; D016178:Granulocyte-Macrophage Colony-Stimulating Factor