The effect of calcipotriol on lesional fibroblasts from patients with active morphoea.
We examined the responsiveness of cultured dermal fibroblasts from biopsies of 6 patients with active morphoea and a similar number of matched controls to the cell proliferation inhibition activity of calcipotriol. Cultured fibroblasts from controls showed no significant response to calcipotriol (at concentrations of 1 x 10(-8) to 1 x 10(-4) M). However, calcipotriol did inhibit the proliferation response of morphoea fibroblasts at all concentrations when compared with controls. There was 4- to 20-fold inhibition in 2 of the morphoea patients when compared with control samples. Four other morphoea samples showed inhibition but to a lesser extent compared with controls.
Bottomley
W W
WW
Department of Dermatology, Leeds General Infirmary, UK.
Jutley
J
J
Wood
E J
EJ
Goodfield
M D
MD
D000293:Adolescent; D000328:Adult; D001706:Biopsy; D002117:Calcitriol / Q000031:analogs & derivatives* / Q000494:pharmacology; D016022:Case-Control Studies; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003879:Dermatologic Agents / Q000494:pharmacology*; D005260:Female; D005347:Fibroblasts / Q000187:drug effects*; D006801:Humans; D008297:Male; D008875:Middle Aged; D012594:Scleroderma, Localized / Q000473:pathology / Q000503:physiopathology*
D003879:Dermatologic Agents; C055085:calcipotriene; D002117:Calcitriol