Characterization of a nuclear protein conferring brefeldin A resistance in Schizosaccharomyces pombe.
The fungal metabolite brefeldin A disrupts protein secretion and causes the redistribution of the Golgi complex to the endoplasmic reticulum. Previously we isolated six genes that, when present in multiple copies, confer brefeldin A resistance to wild type Schizosaccharomyces pombe. Here we describe the characterization of one of these genes, hba1. This gene encodes an essential protein that shares homology with the mammalian protein RanBP1 and the protein encoded by the Saccharomyces cerevisiae gene YRB1 and contains a peptide motif present in several proteins found within the nuclear pore complex. The protein encoded by hba1 is localized to the nucleus, and it was determined that this protein is phosphorylated in vivo. The characterization of hba1 thus demonstrates a novel mechanism of drug resistance in S. pombe.
Turi
T G
TG
Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06510, USA.
Mueller
U W
UW
Sazer
S
S
Rose
J K
JK
D000595:Amino Acid Sequence; D000935:Antifungal Agents / Q000494:pharmacology*; D001483:Base Sequence; D020126:Brefeldin A; D002451:Cell Compartmentation / Q000187:drug effects; D016384:Consensus Sequence; D003517:Cyclopentanes / Q000494:pharmacology*; D017931:DNA Primers / Q000737:chemistry; D004352:Drug Resistance, Microbial; D005656:Fungal Proteins / Q000378:metabolism; D019204:GTP-Binding Proteins / Q000737:chemistry / Q000378:metabolism; D005800:Genes, Fungal; D006056:Golgi Apparatus / Q000187:drug effects; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000737:chemistry / Q000502:physiology*; D010750:Phosphoproteins / Q000502:physiology*; D015183:Restriction Mapping; D012568:Schizosaccharomyces / Q000187:drug effects / Q000235:genetics*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D020931:ran GTP-Binding Protein
D000935:Antifungal Agents; D003517:Cyclopentanes; D017931:DNA Primers; D005656:Fungal Proteins; D009687:Nuclear Proteins; D010750:Phosphoproteins; C091775:ran-binding protein 1; D020126:Brefeldin A; D019204:GTP-Binding Proteins; D020931:ran GTP-Binding Protein