title: High-performance liquid chromatographic determination of urinary 2,5-hexanedione as mono-2,4-dinitrophenylhydrazone using ultraviolet detection.
abstract: The good correlation between exposure to n-hexane and 2,5-hexanedione urinary excretion confers on this diketone an important toxicological meaning. this paper proposes a reversed-phase HPLC method which includes, after acid hydrolysis, a derivatization step of 2,5-hexanedione with 2,4-dinitrophenylhydrazine at 70 degrees C for 20 min. The reaction conditions, such as temperature, reagent concentration and time, are optimized so as to allow the condensation of a single carbonyl group. A linear response was obtained in the 0.19-20.0 mg/l range with a detection limit of 0.03 mg/l, corresponding to a signal-to-noise ratio of 3. A phosphate buffer (pH 3.3)-acetonitrile mixture (50:50) as the eluent and UV detection at 334 nm were used.
authors.affiliation: Istituto di Medicina del Lavoro, Università di Padova, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Gori
authors.forename: G B
authors.initials: GB
authors.lastname: Bartolucci
authors.forename: A
authors.initials: A
authors.lastname: Sturaro
authors.forename: G
authors.initials: G
authors.lastname: Parvoli
authors.forename: L
authors.initials: L
authors.lastname: Doretti
authors.forename: R
authors.initials: R
authors.lastname: Troiano
authors.forename: B
authors.initials: B
authors.lastname: Casetta
mesh_terms: D002800:Cholinesterase Inhibitors / Q000652:urine*; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000706:statistics & numerical data; D006586:Hexanes; D006588:Hexanones / Q000652:urine*; D006801:Humans; D006835:Hydrazones / Q000652:urine*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D016273:Occupational Exposure; D011786:Quality Control; D012016:Reference Values
chemical_list: C001072:2,4-dinitrophenylhydrazone; D002800:Cholinesterase Inhibitors; D006586:Hexanes; D006588:Hexanones; D006835:Hydrazones; C026385:n-hexane; C011269:2,5-hexanedione