title: A one-dimensional (proton and phosphorus) and two-dimensional (proton) in vivo NMR spectroscopic study of reversible global cerebral ischemia.
abstract: The suitability of two-dimensional (2D) proton spectroscopy for monitoring, in vivo, the changes in levels of brain metabolites induced by cerebral ischemia was investigated in an experimental model of 30-min reversible ischemia induced by four-vessel occlusion in the rat. The resulting data were compared with those obtained by one-dimensional (1D) proton and phosphorus spectroscopy. Phosphorus spectra obtained during ischemia showed significant drops in levels of phosphocreatine (-73%), beta-ATP (-60%), and intracellular pH (to 6.30) and an increase in inorganic phosphate level (905%). 1D and 2D proton spectra showed decreases in the N-acetylaspartate/creatine-phosphocreatine ratio that were not significantly different [-21% (1D) and -32% (2D)]. Similarly, the increases in lactate/creatine-phosphocreatine ratio were not significantly different [2,546% (1D) and 3,020% (2D)]. 2D spectroscopy also indicated a decrease in aspartate (-66%) and an increase in the inositol-choline derivative (+124%) pools during ischemia and an increase in alanine pool (+516%) during reperfusion. The glutamate-glutamine pool and taurine content did not change significantly during ischemia but decreased during reperfusion. The glucose level transiently decreased (-67%) during ischemia and increased immediately after (+261%). The levels of all the metabolites investigated returned to control values within 175 min after ischemia. 2D spectroscopy seems to be a reliable method of monitoring the changes in levels of cerebral compounds known to be involved in ischemia.
authors.affiliation: Laboratoire de Recherches Cérébrovasculaires, CNRS URA 641, Université Paris VII, France.
authors.forename: S
authors.initials: S
authors.lastname: Brulatout
authors.forename: P
authors.initials: P
authors.lastname: Méric
authors.forename: I
authors.initials: I
authors.lastname: Loubinoux
authors.forename: J
authors.initials: J
authors.lastname: Borredon
authors.forename: J L
authors.initials: JL
authors.lastname: Corrèze
authors.forename: P
authors.initials: P
authors.lastname: Roucher
authors.forename: B
authors.initials: B
authors.lastname: Gillet
authors.forename: G
authors.initials: G
authors.lastname: Bérenger
authors.forename: J C
authors.initials: JC
authors.lastname: Beloeil
authors.forename: B
authors.initials: B
authors.lastname: Tiffon
authors.forename: J
authors.initials: J
authors.lastname: Mispelter
authors.forename: J
authors.initials: J
authors.lastname: Seylaz
mesh_terms: D000818:Animals; D002545:Brain Ischemia / Q000503:physiopathology*; D006859:Hydrogen; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D010759:Phosphorus Isotopes; D016548:Prosencephalon / Q000098:blood supply*; D011522:Protons; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000503:physiopathology*
chemical_list: D010759:Phosphorus Isotopes; D011522:Protons; D006859:Hydrogen