title: Estimating analytical variances in measurement of polycyclic aromatic hydrocarbons and application to monitoring contaminants in American lobster (Homarus americanus).
abstract: A method is presented for estimating replicate polycyclic aromatic hydrocarbon (PAH) concentrations in American lobster (Homarus americanus) digestive gland tissue based on recoveries of added perdeuterated surrogates from a single satisfactory analysis. PAH concentrations demonstrated a large interanimal variance, even in specimens captured at the same time in the same place. Principal component analysis showed that the variability of the total system of biological variables (carapace length, lobster weight, and digestive gland weight) could be adequately summarized by the first principal component alone in each data set. Ranks provide ordered classification of individuals, allowing data analysis by statistical methods for continuous variables (i.e., analysis of variance). PAH concentrations in individual lobsters were generally highly sensitive to animal size, sex, and fishing area. Efficient monitoring would result from analyzing individual animals of a single sex from a study area, using as small a geographical study area as possible, measuring a single biological variable, and using individual specimens of as narrow a size range as possible.
authors.affiliation: Department of Fisheries and Oceans, Marine Environmental Sciences Division, Maritimes Region, Halifax, Canada.
authors.forename: J F
authors.initials: JF
authors.lastname: Uthe
authors.forename: R K
authors.initials: RK
authors.lastname: Misra
authors.forename: T L
authors.initials: TL
authors.lastname: King
authors.forename: C J
authors.initials: CJ
authors.lastname: Musial
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D005260:Female; D008297:Male; D008121:Nephropidae / Q000737:chemistry*; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis*; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D011084:Polycyclic Aromatic Hydrocarbons; D014874:Water Pollutants, Chemical