Cloning of SEZ-12 encoding seizure-related and membrane-bound adhesion protein.
SEZ-12 is one of the seizure-related cDNAs which was isolated by differential hybridization from primary cultured neurons from the mouse cerebral cortex with or without pentylenetetrazol (PTZ). SEZ-12 expression is transiently down-regulated in the mouse brain by injection of PTZ. To characterize SEZ-12, isolation of full-length cDNA and nucleotide sequence analysis were performed. The deduced amino acid sequence of SEZ-12 revealed that it encodes membrane-bound C-type lectin and has a significant homology to that of human cDNA, DGCR2 and IDD, which were cloned from a balanced translocation breakpoint associated with the DiGeorge syndrome. The isolated cDNA was about 4 kb in length and the message was expressed ubiquitously in various organs with low-abundance. Previously, we also cloned a transmembrane protein which is probably involved in cell-cell interaction by the differential hybridization technique. These findings suggest that transmembrane signaling in neuronal cells may have an important role in PTZ-induced seizure.
Kajiwara
K
K
Department of Molecular and Developmental Science, Tokai University, Kanagawa, Japan.
Nagasawa
H
H
Shimizu-Nishikawa
K
K
Ookura
T
T
Kimura
M
M
Sugaya
E
E
D000595:Amino Acid Sequence; D000818:Animals; D015815:Cell Adhesion Molecules / Q000235:genetics*; D003001:Cloning, Molecular; D003292:Convulsants / Q000494:pharmacology; D018076:DNA, Complementary / Q000235:genetics; D006801:Humans; D037102:Lectins / Q000235:genetics*; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D051379:Mice; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics*; D010433:Pentylenetetrazole / Q000494:pharmacology; D019038:Platelet Glycoprotein GPIb-IX Complex; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
D015815:Cell Adhesion Molecules; D003292:Convulsants; D018076:DNA, Complementary; D037102:Lectins; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D019038:Platelet Glycoprotein GPIb-IX Complex; C094990:adhesion receptor; D010433:Pentylenetetrazole