title: Expression of cytokeratin 8 in basal cell carcinoma: a comparative immunohistochemical and immunoelectron microscopy study.
abstract: The comparative study reported here was undertaken in order to resolve the discrepancies in the detection of cytokeratin (Ck) 8 reported in previous studies. The expression of Ck 8 was compared in 6 basal cell carcinomas (BCCs) using immunohistochemical and immunoelectron microscopic techniques and a panel of 4 different commercially available monoclonal antibodies (MoAbs). The results of this comparative study demonstrated not only that the consistent expression of Ck 8 using one of the MoAbs in immunohistochemistry was confirmed by immunoelectron microscopy, but that the inconsistent expression of Ck 8 observed using two other MoAbs was also confirmed. One of the MoAbs did not show any staining at all. The inability of this MoAb to detect the expression of Ck 8 using either of the techniques also indicated that this MoAb may be directed against an epitope of Ck8 that is not detectable in BCC in situ.
authors.affiliation: Department of Dermato-Venereology, Erasmus University, Rotterdam, The Netherlands.
authors.forename: A J
authors.initials: AJ
authors.lastname: Kooy
authors.forename: B
authors.initials: B
authors.lastname: Tank
authors.forename: T A
authors.initials: TA
authors.lastname: de Jong
authors.forename: V D
authors.initials: VD
authors.lastname: Vuzevski
authors.forename: F T
authors.initials: FT
authors.lastname: Bosman
authors.forename: T
authors.initials: T
authors.lastname: van Joost
mesh_terms: D000368:Aged; D000911:Antibodies, Monoclonal; D002280:Basal Cell Carcinoma / Q000737:chemistry* / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007633:Keratins / Q000032:analysis*; D008297:Male; D016253:Microscopy, Immunoelectron; D008875:Middle Aged; D012878:Skin Neoplasms / Q000737:chemistry* / Q000473:pathology
chemical_list: D000911:Antibodies, Monoclonal; D007633:Keratins