Up-regulation of rat adrenomedullin gene expression by endotoxin: relation to nitric oxide synthesis.
The effect of bacterial endotoxin (LPS) on adrenomedullin (AM) gene expression was investigated in cultured rat aortic vascular smooth muscle (VSM) cells and in tissues from anesthetized rats. The addition of LPS together with interferon-gamma to VSM cells resulted in a marked increase in the abundance of AM mRNA as well as the appearance of mRNA for the inducible isoform of nitric oxide (NO) synthase (iNOS). Intravenous injection of LPS into rats also increased AM mRNA abundance and induced iNOS mRNA in lung, heart, liver, and kidney. AM significantly enhanced NO synthesis evoked by LPS and interferon-gamma in cultured VSM cells. These data suggest that AM may contribute to circulatory failure during endotoxin shock, in part, by modulating NO synthesis.
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Department of Endocrinology, Dokkyo University School of Medicine, Tochigi, Japan.
Hattori
Y
Y
Kasai
K
K
Shimoda
S
S
Gross
S S
SS
D053607:Adrenomedullin; D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D017931:DNA Primers; D005786:Gene Expression Regulation / Q000187:drug effects*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008297:Male; D008969:Molecular Sequence Data; D009569:Nitric Oxide / Q000096:biosynthesis*; D010455:Peptides / Q000097:blood / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011863:Radioimmunoassay; D051381:Rats; D017208:Rats, Wistar; D015854:Up-Regulation
D017931:DNA Primers; D008070:Lipopolysaccharides; D010455:Peptides; D012333:RNA, Messenger; D053607:Adrenomedullin; D009569:Nitric Oxide