Evidence for involvement of equine herpesvirus 1 glycoprotein B in cell-cell fusion.
Monoclonal antibodies specific for equine herpesvirus 1 (EHV-1) glycoproteins (gB, gD, gp2 and a cleaved translation product of gene 71) were tested for ability to inhibit cell-cell fusion as measured by syncytium formation in EHV-1 infected cell cultures. Syncytium formation was inhibited by a complement-dependent neutralising antibody (7B10) which recognised the large subunit of EHV-1 gB. This indicated that EHV-1 gB, in common with gB homologues of herpes simplex virus and other herpesviruses, plays a role in the cell-cell fusion process.
Wellington
J E
JE
School of Biological Sciences, Macquarie University, Sydney, New South Wales, Australia.
Love
D N
DN
Whalley
J M
JM
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D002459:Cell Fusion / Q000187:drug effects; D002460:Cell Line; D005814:Genes, Viral; D015726:Giant Cells; D004861:Herpesvirus 1, Equid / Q000502:physiology*; D006736:Horses; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology; D014759:Viral Envelope Proteins / Q000096:biosynthesis / Q000276:immunology / Q000502:physiology*
D000911:Antibodies, Monoclonal; D014759:Viral Envelope Proteins