Immunocytochemical mapping of a C-terminus anti-peptide antibody to the GABA receptor subunit, RDL in the nervous system in Drosophila melanogaster.
An antibody raised against a peptide based on the C-terminal derived amino acid sequence from a cloned Drosophila melanogaster (fruit fly) gene, Rdl (resistant to dieldrin), was used to investigate localization of a GABA receptor subunit in adult male D. melanogaster. Many regions in the brain and thoracic ganglia were stained with this antibody. For example, staining was detected in the medulla, lobula and lobular plate optic neurpiles. Also stained were the antennal lobe glomeruli, the ellipsoid body of the central complex and the mushroom bodies. These results suggest possible roles for an RDL-like GABA receptor subunit in the processing of olfactory, visual and mechanosensory information in the nervous system of D. melanogaster.
Harrison
J B
JB
Babraham Institute Laboratory of Molecular Signalling, Cambridge, UK.
Chen
H H
HH
Sattelle
E
E
Barker
P J
PJ
Huskisson
N S
NS
Rauh
J J
JJ
Bai
D
D
Sattelle
D B
DB
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000032:analysis / Q000276:immunology; D000917:Antibody Formation; D000918:Antibody Specificity; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000033:anatomy & histology*; D017952:Ganglia, Invertebrate / Q000737:chemistry; D007150:Immunohistochemistry; D007305:Insecticide Resistance; D008969:Molecular Sequence Data; D009420:Nervous System / Q000737:chemistry; D009899:Optic Lobe, Nonmammalian / Q000737:chemistry; D010455:Peptides / Q000032:analysis / Q000276:immunology; D011963:Receptors, GABA-A / Q000032:analysis / Q000276:immunology*
D000906:Antibodies; D029721:Drosophila Proteins; D010455:Peptides; C082030:Rdl protein, Drosophila; D011963:Receptors, GABA-A