IL-1Rrp is a novel receptor-like molecule similar to the type I interleukin-1 receptor and its homologues T1/ST2 and IL-1R AcP.
A novel member of the interleukin-1 receptor family has been cloned by polymerase chain reaction using degenerate oligonucleotide primers derived from regions of sequence conservation, using as template a yeast artificial chromosome known to contain both interleukin-1 (IL-1) receptors and T1/ST2. The new receptor, called IL-1 receptor-related protein or IL-1Rrp, fails to bind any of the known IL-1 ligands. A chimeric receptor, in which the IL-1Rrp cytoplasmic domain is fused to the extracellular and transmembrane regions of the IL-1 receptor, responds to IL-1 following transfection into COS cells by activation of NFkappaB and induction of IL-8 promoter function.
Parnet
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Immunex Corporation, Seattle, Washington 98101, USA.
Garka
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Bonnert
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Dower
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Sims
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D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D003593:Cytoplasm / Q000378:metabolism; D017931:DNA Primers; D006801:Humans; D053727:Interleukin-18 Receptor alpha Subunit; D016209:Interleukin-8 / Q000096:biosynthesis / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D016328:NF-kappa B / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D014176:Protein Biosynthesis; D011506:Proteins / Q000737:chemistry*; D017472:Receptors, Interleukin-1 / Q000096:biosynthesis* / Q000737:chemistry*; D053724:Receptors, Interleukin-18; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000737:chemistry; D017386:Sequence Homology, Amino Acid; D014162:Transfection
D017931:DNA Primers; C508572:IL18R1 protein, human; C508573:Il18r1 protein, mouse; D053727:Interleukin-18 Receptor alpha Subunit; D016209:Interleukin-8; D016328:NF-kappa B; D011506:Proteins; D017472:Receptors, Interleukin-1; D053724:Receptors, Interleukin-18; D011993:Recombinant Fusion Proteins