Biased T cell receptor V gene usage in tissues with periodontal disease.
In an attempt to characterize TCR V gene usage in human periodontally diseased tissue, V alpha 2, V beta 5.2-3, V beta 5.3, V beta 5.1, V beta 6.7, V beta 6.7, V beta 8 and V beta 12.1 expressions were examined. Serial cryostat sections obtained from 20 periodontitis and 9 gingivitis biopsies were then reacted with monoclonal antibodies directed to each repertoire. The technique was combined with a sensitive alkaline phosphatase-anti-alkaline phosphatase method. Peripheral blood was obtained from 10 periodontitis and 2 gingivitis patients. TCR repertoire was also quantified by flow cytofluorography with FITC-conjugated antibodies. Cells displaying binding of each antibody were counted. The proportions to CD3-positive cells were then calculated. The pattern of each TCR V gene product expression in inflamed gingiva exhibited individual variation, nevertheless, a consistent pattern emerged. The V beta 5 subfamily and V beta 6.7 were frequently used repertoires in gingiva, whereas the V alpha 2 and V beta 8 subfamily were underexpressed in most cases. Furthermore, the TCR V gene product expression in gingival tissue was biased compared with autologous peripheral blood. Three of 10 periodontitis subjects showed 1 or 2 strikingly overrepresented repertoire comparatively with autologous blood. In these 3 subjects V beta 6.7 was overexpressed in two cases and 5.2-3, V beta 8 and V beta 12.1 were overexpressed in one case. These results suggest that gingival T-cells are not randomly mobilized from peripheral blood and that local events influence the TCR repertoire at the level of T-cell recruitment or T-cell expansion.
Nakajima
T
T
Department of Periodontology, Niigata University School of Dentistry, Japan.
Yamazaki
K
K
Hara
K
K
D000328:Adult; D000911:Antibodies, Monoclonal; D017252:CD3 Complex; D000939:Epitopes; D005434:Flow Cytometry; D015870:Gene Expression; D005891:Gingivitis / Q000276:immunology*; D006801:Humans; D007135:Immunoglobulin Variable Region / Q000235:genetics; D007150:Immunohistochemistry; D016130:Immunophenotyping; D008875:Middle Aged; D010518:Periodontitis / Q000276:immunology*; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D018709:Statistics, Nonparametric; D016176:T-Lymphocyte Subsets
D000911:Antibodies, Monoclonal; D017252:CD3 Complex; D000939:Epitopes; D007135:Immunoglobulin Variable Region; D016693:Receptors, Antigen, T-Cell, alpha-beta