DNA minor groove accessibility in the nucleosome core deduced from specific interactions with DAPI.
Equilibrium binding properties of DAPI for purified nucleosome cores were derived from fluorescence intensity enhancement. Both the affinity and the number of sites of the primary, minor groove binding are preserved with respect to the corresponding isolated DNA, suggesting that the capacity for specific interactions of the minor groove may well be exerted in chromatin.
Caneva
R
R
Centro di Studio per gli Acidi Nucleici del CNR, Dipartimento di Genetica e Biologia Molecolare, Rome,Italy.
Rossetti
L
L
Savino
M
M
D000818:Animals; D001665:Binding Sites; D002645:Chickens; D004247:DNA / Q000378:metabolism*; D005456:Fluorescent Dyes; D007211:Indoles / Q000378:metabolism*; D009707:Nucleosomes / Q000378:metabolism*; D013050:Spectrometry, Fluorescence
D005456:Fluorescent Dyes; D007211:Indoles; D009707:Nucleosomes; C007293:DAPI; D004247:DNA