Participation of gap-junctional cell communication on the adaptive response in human cells induced by low dose of X-rays.
To investigate the radioadaptive response of normal cells to low-dose radiation, we irradiated human embryonic (HE) cells and HeLa cells with low-dose X-rays and examined the changes in sensitivity to subsequent high-dose X-irradiation using the trypan blue dye-exclusion test. When HE cells were irradiated by 200 cGy, the growth ratio of the living cells 5 days after the irradiation decreased to 37% of that of the cells which received no X-irradiation. When the cells received a conditioning irradiation of 10-20 cGy 4 h before the irradiation of 200 cGy, the relative growth ratios increased significantly to 45-53%, and a peak was reached at a conditioning dose of 13 cGy to the cells. This conditioning effect was not observed in LeLa cells. When the HE cells were suspended in a Ca2+ ion-free medium or TPA added to the medium while receiving the conditioning irradiation of 13 cGy, the effect of the conditioning dose was not observed. This indicates that normal cells show an adaptive response to low-dose radiation and become more radioresistant. These results suggest that gap junctional intercellular communication may play a role in radioadaptive responses in human cells.
Ishii
K
K
Radiation Safety Group, Central Research Institute of Electric Power Industry, Tokyo, Japan.
Watanabe
M
M
D000222:Adaptation, Physiological / Q000528:radiation effects; D002118:Calcium / Q000494:pharmacology; D002450:Cell Communication / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects*; D002455:Cell Division / Q000528:radiation effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D004249:DNA Damage; D004307:Dose-Response Relationship, Radiation; D005456:Fluorescent Dyes; D017629:Gap Junctions / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects*; D006367:HeLa Cells; D006801:Humans; D007546:Isoquinolines; D011836:Radiation Tolerance; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D005456:Fluorescent Dyes; D007546:Isoquinolines; C017475:lucifer yellow; D013755:Tetradecanoylphorbol Acetate; D002118:Calcium