In vivo detection of mouse liver nitric oxide generation by spin trapping electron paramagnetic resonance spectroscopy.
Nitric oxide, a paramagnetic molecule synthesized in biological systems, plays an important role in many pathophysiological processes. In vivo electron paramagnetic resonance spectroscopy/imaging could be a useful tool to study, in situ and in real time, nitric oxide generation. In this study the intracellular production of nitric oxide in tissues of living septic-shock mouse was detected by the spin trapping technique in combination with electron paramagnetic resonance spectroscopy. A lipophilic spin trap agent was used and nitric oxide formation was determined by the intensity of its iron-mononitrosyl complex. Among all examined tissues at 20 degrees C, the highest signal intensity of the trapped nitric oxide was found in the liver homogenates (n = 5). The amount of complex found in the kidneys was about 40% of that found in the liver. In the brain and lung, around 10% was found. This study reports, for the first time, the in vivo detection of nitric oxide generation in the upper abdomen of septic-shock mice (n = 3). Within 1 h after the trap injection, the signal was stable, indicating that the formation had reached a steady state.
Quaresima
V
V
Department of Sciences and Biomedical Technologies, University of L'Aquila, Italy. vale@univaq.it.
Takehara
H
H
Tsushima
K
K
Ferrari
M
M
Utsumi
H
H
D000818:Animals; D004050:Ditiocarb / Q000031:analogs & derivatives / Q000493:pharmacokinetics; D004578:Electron Spin Resonance Spectroscopy; D005260:Female; D005296:Ferrous Compounds / Q000493:pharmacokinetics; D008099:Liver / Q000378:metabolism*; D051379:Mice; D008813:Mice, Inbred ICR; D009569:Nitric Oxide / Q000032:analysis / Q000096:biosynthesis*; D019097:Spin Trapping; D014018:Tissue Distribution
D005296:Ferrous Compounds; D009569:Nitric Oxide; D004050:Ditiocarb