Predominant involvement of CD8+CD28- lymphocytes in human immunodeficiency virus-specific cytotoxic activity.
Distinct functional CD8+ T-cell populations have been observed during human immunodeficiency virus (HIV) infection. One of these functions is the inhibition of viral replication by a noncytotoxic mechanism, which was shown to be mediated by the CD8+CD28+ subpopulation. On the other hand, CD8+ T cells exert an HIV-specific cytotoxic activity. The present study shows that CD8+CD28- lymphocytes display this HIV-specific cytotoxic activity, which is detectable immediately after the cells are purified from peripheral blood. The CD28- population is also able to proliferate and to retain its cytotoxic activity after in vitro restimulation with autologous blast cells. Finally, HIV-specific cytotoxic T cells can be obtained in vitro from the CD8+CD28+ population.
Fiorentino
S
S
Laboratoire d'Immunologie des Pathologies Infectieuses et Tumorales, Unité 445 de l'Institut National de la Santé et de la Recherche Médicale, Hôpital Cochin, Paris, France.
Dalod
M
M
Olive
D
D
Guillet
J G
JG
Gomard
E
E
D018106:CD28 Antigens / Q000276:immunology*; D016827:CD8 Antigens / Q000276:immunology*; D002478:Cells, Cultured; D003601:Cytotoxicity Tests, Immunologic; D006678:HIV / Q000276:immunology*; D006679:HIV Seropositivity / Q000097:blood / Q000276:immunology; D006801:Humans; D013602:T-Lymphocytes, Cytotoxic / Q000166:cytology / Q000276:immunology*
D018106:CD28 Antigens; D016827:CD8 Antigens