Characterization of Streptomyces venezuelae ATCC 10595 rRNA gene clusters and cloning of rrnA.
Streptomyces venezuelae ATCC 10595 harbors seven rRNA gene clusters which can be distinguished by BglII digestion. The three rRNA genes present in each set are closely linked with the general structure 16S-23S-5S. We cloned rrnA and sequenced the 16S-23S spacer region and the region downstream of the 5S rRNA gene. No tRNA gene was found in these regions.
La Farina
M
M
Dipartimento di Biologia Cellulare e dello Sviluppo, Università di Palermo, Italy.
Stira
S
S
Mancuso
R
R
Grisanti
C
C
D003001:Cloning, Molecular; D004275:DNA, Ribosomal / Q000235:genetics*; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D005810:Multigene Family; D009693:Nucleic Acid Hybridization; D015347:RNA Probes; D012335:RNA, Ribosomal / Q000235:genetics*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D012341:RNA, Ribosomal, 5S / Q000235:genetics; D013045:Species Specificity; D013302:Streptomyces / Q000235:genetics*
D004275:DNA, Ribosomal; D015347:RNA Probes; D012335:RNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S; D012341:RNA, Ribosomal, 5S