Isolation of a novel gene down-regulated by v-src.
We have isolated a novel gene which was expressed in normal rat cells, but completely suppressed in cells transformed by v-src. The molecularly cloned cDNA was about 1.8 kb in size, containing an open reading frame composed of 464 amino acid residues. DNA sequence analysis showed that there was no corresponding gene in the data bases. Besides the suppression of gene expression in the v-src transformed cells, its expression was also strongly suppressed in cells transformed by other oncogenes such as v-abl, v-fps, v-mos, v-sis, v-K-ras, and polyomavirus middle T, but not affected in cells transformed by human papillomavirus type 16 E6E7 and polyomavirus large T. We named the gene drs for a gene down-regulated by v-src.
Pan
J
J
Department of Tumor Virology, Research Institute for Microbial Diseases, Osaka University, Japan.
Nakanishi
K
K
Yutsudo
M
M
Inoue
H
H
Li
Q
Q
Oka
K
K
Yoshioka
N
N
Hakura
A
A
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002461:Cell Line, Transformed; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D002472:Cell Transformation, Viral / Q000235:genetics; D016384:Consensus Sequence / Q000235:genetics; D018076:DNA, Complementary / Q000737:chemistry; D015536:Down-Regulation / Q000235:genetics*; D005796:Genes; D016391:Genes, src / Q000235:genetics*; D006801:Humans; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D009857:Oncogenes / Q000235:genetics; D051381:Rats; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics; D019087:Selectins / Q000737:chemistry / Q000235:genetics; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D008565:Membrane Proteins; C486297:SRPX protein, human; D019087:Selectins; C099179:Srpx protein, rat