Neutralization of human cytomegalovirus by human CD13-specific antibodies.
The mechanisms of inhibition of cytomegalovirus (CMV) infection by human CD13 (aminopeptidase N)-specific antibodies were studied. These antibodies protect CD13-negative and -positive cells from CMV infection only if incubated with the virus inoculum, suggesting they bind to CMV virions. The association of a CD13-like molecule with virions was further supported by the transfer of CD13 immunoreactivity to the surface of CD13-negative cells upon binding of CMV; the binding of CD13-specific antibodies directly to the surface of CMV virions; and the presence of anti-CD13 immunoreactive bands, including one with mobility similar to that of native cellular CD13 on immunoblots of proteins of purified CMV particles. Importantly, CD13-specific antibodies neutralize CMV in urine of neonates with congenital CMV, indicating that the CD13-like molecule is associated with natural CMV and not acquired in vitro. These studies demonstrate that a CD13-like molecule is associated with CMV particles and may be important in CMV pathogenesis.
Giugni
T D
TD
Division of Pediatrics, City of Hope National Medical Center, Duarte, California 91010-0269, USA.
Söderberg
C
C
Ham
D J
DJ
Bautista
R M
RM
Hedlund
K O
KO
Möller
E
E
Zaia
J A
JA
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000914:Antibodies, Viral / Q000276:immunology*; D000918:Antibody Specificity; D018826:CD13 Antigens / Q000276:immunology*; D002460:Cell Line; D003429:Cross Reactions; D003587:Cytomegalovirus / Q000276:immunology*; D003586:Cytomegalovirus Infections / Q000151:congenital / Q000276:immunology / Q000821:virology; D000939:Epitopes / Q000032:analysis; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D051379:Mice; D009500:Neutralization Tests; D014771:Virion / Q000378:metabolism
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000939:Epitopes; D018826:CD13 Antigens