The RNA component of mitochondrial ribonuclease P from Aspergillus nidulans.
Several RNA molecules that copurified with Aspergillus nidulans mitochondrial ribonuclease (RNase) P were identified [Lee, Y C., Lee, B. J., Hwang, D. S. & Kang, H. S. (1996) Eur J. Biochem. 235, 289-296], and their partial sequences were determined. Using an oligonucleotide probe, we cloned and mapped the gene encoding this putative RNA component of RNase P (RNase P-RNA), situated between URFA3 (unidentified reading frame A3) and cobA (apocytochrome b) genes in the mitochondrial genome of A. nidulans. The gene is extremely (A+T)-rich and contains two regions of sequence similarity conserved among the known mitochondrial RNase P-RNAs and the eubacterial RNase P-RNAs. The determination of 5' and 3' termini by primer extension and sequencing indicated that the length of the RNA transcript is 232 nucleotides. Northern-blot analysis revealed that its only subcellular location was the mitochondria. Two RNase P-RNA fragments of 110 nucleotides and 80 nucleotides, each containing one of the two conserved regions, could be recovered from the nuclease-treated enzyme without significant loss of activity. The sizes of these fragments appeared to be the minimum lengths required for the vitro activity of the enzyme.
Lee
Y C
YC
Department of Microbiology, College of Natural Sciences, Seoul National University, Korea.
Lee
B J
BJ
Kang
H S
HS
D001233:Aspergillus nidulans / Q000201:enzymology* / Q000235:genetics*; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D016384:Consensus Sequence; D017124:Conserved Sequence; D015342:DNA Probes / Q000235:genetics; D004722:Endoribonucleases / Q000235:genetics* / Q000302:isolation & purification; D005800:Genes, Fungal; D008928:Mitochondria / Q000201:enzymology; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D016337:RNA, Catalytic / Q000235:genetics* / Q000302:isolation & purification; D012331:RNA, Fungal / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D043262:Ribonuclease P; D015003:Yeasts / Q000201:enzymology / Q000235:genetics
D015342:DNA Probes; D016337:RNA, Catalytic; D012331:RNA, Fungal; D004722:Endoribonucleases; D043262:Ribonuclease P