DNA replication fork pause sites dependent on transcription.
Replication fork pause (RFP) sites transiently arresting replication fork movement were mapped to transfer RNA (tRNA) genes of Saccharomyces cerevisiae in vivo. RFP sites are polar, stalling replication forks only when they oppose the direction of tRNA transcription. Mutant tRNA genes defective in assembly of transcription initiation complexes and a temperature-sensitive RNA polymerase III mutant (rpc160-41) defective in initiation of transcription do not stall replication forks, suggesting that transcription is required for RFP activity.
Deshpande
A M
AM
Department of Micobiology and Molecular Genetics, UMDNJ Medical School and UMDNJ-Graduate School of Biomedical Sciences, Newark, NJ 07103, USA.
Newlon
C S
CS
D001483:Base Sequence; D003001:Cloning, Molecular; D004261:DNA Replication; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D017354:Point Mutation; D012320:RNA Polymerase III / Q000378:metabolism; D012331:RNA, Fungal / Q000235:genetics*; D012343:RNA, Transfer / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid; D018741:Replication Origin; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D017384:Sequence Deletion; D013696:Temperature; D014157:Transcription Factors / Q000378:metabolism; D014158:Transcription, Genetic
D012331:RNA, Fungal; D014157:Transcription Factors; D012343:RNA, Transfer; D012320:RNA Polymerase III