Protective Role of TNF-alpha in respiratory syncytial virus infection in vitro and in vivo.
Respiratory syncytial virus (RSV) infection causes substantial morbidity in young children and immunocompromised adults, yet its pathogenesis is poorly understood. Because the proinflammatory cytokine tumor necrosis factor-alpha (TNF-alpha) may be important in host response to viral infection, HEp-2 cells were treated with TNF-alpha and mice were given TNF-alpha antibody before RSV infection. Pretreatment of HEp-2 cells with TNF-alpha inhibited RSV replication as determined by cytopathic effect. Respiratory syncytial virus-infected BALB/c mice treated with antibody to TNF-alpha had greater maximal weight loss and slower recovery time than control mice. These results suggest a protective role for TNF-alpha in RSV infection.
Neuzil
K M
KM
Departmemt of Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee 37232-2605, USA.
Tang
Y W
YW
Graham
B S
BS
D000818:Animals; D000906:Antibodies / Q000276:immunology; D001835:Body Weight; D006528:Carcinoma, Hepatocellular; D003588:Cytopathogenic Effect, Viral; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D018357:Respiratory Syncytial Virus Infections / Q000503:physiopathology*; D012136:Respiratory Syncytial Viruses / Q000187:drug effects / Q000502:physiology*; D013047:Specific Pathogen-Free Organisms; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology / Q000494:pharmacology / Q000502:physiology*; D010948:Viral Plaque Assay; D014779:Virus Replication
D000906:Antibodies; D014409:Tumor Necrosis Factor-alpha