Isolation and identification of a diuretic hormone from the mealworm Tenebrio molitor.
A diuretic hormone of unusual structure was isolated from extracts of whole heads of the mealworm Tenebrio molitor. The hormone is a 37-aa peptide of 4371 Da, with the sequence SPTISITAPIDVLRKTWEQERARKQMVKNREFLNSLN. This peptide increases cAMP production in Malpighian tubules of T. molitor. The amino acid sequence reveals that this peptide is a member of the family of sauvagine/corticotropin-releasing factor/urotensin I-related insect diuretic hormones. The C-terminal sequence of this peptide is quite different from other members of this family, which have a hydrophobic C terminus (isoleucinamide or valinamide). When aligned comparably, T. molitor diuretic hormone has a more hydrophilic C terminus, leucylasparagine (free acid). In contrast to all other known diuretic hormones of this family, this peptide has exceptionally low stimulatory activity on cAMP production in Malpighian tubules of Manduca sexta. However, at nanomolar concentrations it stimulates cAMP production in Malpighian tubules of T. molitor. Diuretic hormones of this family have been isolated previously from Lepidoptera, Orthoptera, Dictyoptera, and Diptera. This appears to be the first diuretic hormone isolated from a coleopteran insect.
Furuya
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K
Department of Biochemistry, University of Nevada, Reno 89557, USA.
Schegg
K M
KM
Wang
H
H
King
D S
DS
Schooley
D A
DA
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D003346:Corticotropin-Releasing Hormone / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology; D000242:Cyclic AMP / Q000378:metabolism; D004231:Diuresis; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007301:Insect Hormones / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology; D008317:Malpighian Tubules / Q000187:drug effects / Q000502:physiology; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000302:isolation & purification; D051381:Rats; D017386:Sequence Homology, Amino Acid; D013712:Tenebrio / Q000502:physiology*
D000596:Amino Acids; D007301:Insect Hormones; D010446:Peptide Fragments; C097336:diuretic hormone, mealworm; D003346:Corticotropin-Releasing Hormone; D000242:Cyclic AMP