title: Schizosaccharomyces pombe rad23 is allelic with swi10, a mating-type switching/radioresistance gene that shares sequence homology with human and mouse ERCC1.
abstract: Schizosaccharomyces pombe (Sp) rad23-1 mutant cells are extremely sensitive to UV light and ionizing radiation. A genomic DNA fragment that contains wild-type (wt) rad23 has been cloned. The DNA sequence of this cloned gene has been determined and was found to be identical to the previously characterized mating-type switching/radioresistance gene, swi10. Complementation tests between rad23-1 and swi10-154 mutant cells exclusively produce UV-sensitive progeny and confirm that these two genes are allelic. The DNA sequences of rad23-1 and swi10-154 reveal that each contains a single, unique point mutation. In rad23-1, Glu231 changes to a stop codon, resulting in the production of a truncated protein. In swi10-154, a G to A transition mutation is within a splice consensus sequence for intron 1. Therefore, the corresponding mRNA is incapable of being processed appropriately.
authors.affiliation: Center for Radiological Research, Columbia University, New York, NY 10032, USA.
authors.forename: H
authors.initials: H
authors.lastname: Hang
authors.forename: D N
authors.initials: DN
authors.lastname: Hager
authors.forename: L
authors.initials: L
authors.lastname: Goriparthi
authors.forename: K M
authors.initials: KM
authors.lastname: Hopkins
authors.forename: H
authors.initials: H
authors.lastname: Shih
authors.forename: H B
authors.initials: HB
authors.lastname: Lieberman
mesh_terms: D000483:Alleles; D000818:Animals; D001483:Base Sequence; D003062:Codon / Q000235:genetics; D004252:DNA Mutational Analysis; D004260:DNA Repair; D004271:DNA, Fungal / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D004720:Endonucleases; D005656:Fungal Proteins / Q000235:genetics*; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009154:Mutation; D011506:Proteins / Q000235:genetics*; D011836:Radiation Tolerance / Q000235:genetics; D012568:Schizosaccharomyces / Q000235:genetics* / Q000528:radiation effects; D029702:Schizosaccharomyces pombe Proteins; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D014466:Ultraviolet Rays
chemical_list: D003062:Codon; D004271:DNA, Fungal; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D011506:Proteins; C490845:RHP23 protein, S pombe; C079010:SWI10 protein, S pombe; D029702:Schizosaccharomyces pombe Proteins; C071447:ERCC1 protein, human; D004720:Endonucleases; C499360:Ercc1 protein, mouse