title: Dibefurin, a novel fungal metabolite inhibiting calcineurin phosphatase activity.
abstract: The novel calcineurin inhibitor, dibefurin, has been isolated from the fungal culture AB 1650I-759. The isolation was bioactivity-directed fractionation using an assay which measures the phosphatase activity of calcineurin. The compound was purified by countercurrent, reverse phase and gel filtration chromatographies. Several studies, including crystallographic, NMR and MS, revealed that dibefurin is a novel dimeric compound of a unique structural type.
authors.affiliation: Abbott Laboratories, Abbott Park, Illinois 60064, USA.
authors.forename: G M
authors.initials: GM
authors.lastname: Brill
authors.forename: U
authors.initials: U
authors.lastname: Premachandran
authors.forename: J P
authors.initials: JP
authors.lastname: Karwowski
authors.forename: R
authors.initials: R
authors.lastname: Henry
authors.forename: D K
authors.initials: DK
authors.lastname: Cwik
authors.forename: L M
authors.initials: LM
authors.lastname: Traphagen
authors.forename: P E
authors.initials: PE
authors.lastname: Humphrey
authors.forename: M
authors.initials: M
authors.lastname: Jackson
authors.forename: J J
authors.initials: JJ
authors.lastname: Clement
authors.forename: N S
authors.initials: NS
authors.lastname: Burres
authors.forename: S
authors.initials: S
authors.lastname: Kadam
authors.forename: R H
authors.initials: RH
authors.lastname: Chen
authors.forename: J B
authors.initials: JB
authors.lastname: McAlpine
mesh_terms: D001572:Benzofurans / Q000737:chemistry / Q000494:pharmacology*; D019703:Calcineurin; D002148:Calmodulin-Binding Proteins / Q000037:antagonists & inhibitors*; D002850:Chromatography, Gel; D018360:Crystallography, X-Ray; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D005285:Fermentation; D005658:Fungi / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D010749:Phosphoprotein Phosphatases / Q000037:antagonists & inhibitors*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D001572:Benzofurans; D002148:Calmodulin-Binding Proteins; D004791:Enzyme Inhibitors; C099199:dibefurin; D019703:Calcineurin; D010749:Phosphoprotein Phosphatases