title: Kinetics of pyrimidine(6-4)pyrimidone photoproduct repair in Escherichia coli.
abstract: We compared the removal of pyrimidine(6-4)pyrimidone photoproducts [(6-4) photoproducts] and cyclobutane pyrimidine dimers (CPDs) from the genome of repair-proficient Escherichia coli, using monoclonal antibodies specific for each type of lesion. We found that (6-4) photoproducts were removed at a higher rate than CPDs in the first 30 min following a moderate UV dose (40 J/m2). The difference in rates was less than that typically reported for cultured mammalian cells, in which the removal of (6-4) photoproducts is far more rapid than that of CPDs.
authors.affiliation: Department of Biological Sciences, Stanford University, California 94305-5020, USA.
authors.forename: D R
authors.initials: DR
authors.lastname: Koehler
authors.forename: J
authors.initials: J
authors.lastname: Courcelle
authors.forename: P C
authors.initials: PC
authors.lastname: Hanawalt
mesh_terms: D004249:DNA Damage; D004260:DNA Repair; D004307:Dose-Response Relationship, Radiation; D004926:Escherichia coli / Q000235:genetics* / Q000528:radiation effects; D007700:Kinetics; D011740:Pyrimidine Dimers / Q000276:immunology / Q000378:metabolism*; D014466:Ultraviolet Rays
chemical_list: D011740:Pyrimidine Dimers; C058406:pyrimidine-pyrimidone dimer