title: Adhesion proteins increase cellular attachment, follicle-stimulating hormone receptors, and progesterone production in cultured porcine granulosa cells.
abstract: We sought to determine the influence of different constituents of the extracellular matrix on porcine granulosa cell function by assessing cellular attachment, cellular morphology, follicle-stimulating hormone (FSH) receptors, and progesterone production. Cells from immature porcine ovarian follicles were cultured for up to 6 days in serum-free medium containing porcine FSH (pFSH, 10 ng/ml) in culture dishes either uncoated or coated with one of the following adhesion proteins: gelatin (1 mg/cm2), fibronectin (1 microgram/cm2), laminin (1 microgram/cm2), type I collagen (10 micrograms/cm2), or type IV collagen (7.8 micrograms/cm2). Fibronectin, laminin, type I collagen, and type IV collagen increased cellular attachment significantly (P < 0.05). All adhesion proteins except gelatin influenced cellular morphology. Cells cultured on laminin or type IV collagen formed dense clusters of rounded cells. Cells cultured in dishes coated with each adhesion protein except gelatin had higher 125I-pFSH binding per cell than cells cultured in uncoated dishes, with increases of 7- to 12-fold over control (P < 0.05). All adhesion proteins increased progesterone production, ranging from 10- to 50-fold over control (P < 0.05). In summary, not only did adhesion proteins increase attachment to the dishes but they also increased FSH receptors and differentiated function (progesterone production) of granulosa cells from immature porcine ovarian follicles.
authors.affiliation: Department of Obstetrics and Gynecology, University of Cincinnati College of Medicine, Ohio 45267-0526, USA.
authors.forename: C K
authors.initials: CK
authors.lastname: Sites
authors.forename: B
authors.initials: B
authors.lastname: Kessel
authors.forename: A R
authors.initials: AR
authors.lastname: LaBarbera
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000494:pharmacology*; D002478:Cells, Cultured; D003094:Collagen / Q000494:pharmacology; D005260:Female; D005353:Fibronectins / Q000494:pharmacology; D005640:Follicle Stimulating Hormone / Q000378:metabolism; D005780:Gelatin / Q000494:pharmacology; D006107:Granulosa Cells / Q000378:metabolism*; D007797:Laminin / Q000494:pharmacology; D011374:Progesterone / Q000096:biosynthesis*; D011962:Receptors, FSH / Q000378:metabolism*; D013552:Swine; D013997:Time Factors
chemical_list: D015815:Cell Adhesion Molecules; D005353:Fibronectins; D007797:Laminin; D011962:Receptors, FSH; D011374:Progesterone; D005780:Gelatin; D005640:Follicle Stimulating Hormone; D003094:Collagen