title: Attenuated vaccinia virus-circumsporozoite protein recombinants confer protection against rodent malaria.
abstract: NYVAC-based vaccinia virus recombinants expressing the circumsporozoite protein (CSP) were evaluated in the Plasmodium berghei rodent malaria model system. Immunization of mice with a NYVAC-based CSP recombinant elicited a high level of protection (60 to 100%). Protection did not correlate with CS repeat-specific antibody responses and was abrogated by in vivo CD8+ T-cell depletion. Protection was not enhanced by modification of the subcellular localization of CSP. These results suggest the potential of poxvirus-based vectors for the development of vaccine candidates for human malaria.
authors.affiliation: Department of Immunology, Walter Reed Army Institute of Research, Washington, DC 20307, USA.
authors.forename: D E
authors.initials: DE
authors.lastname: Lanar
authors.forename: J A
authors.initials: JA
authors.lastname: Tine
authors.forename: C
authors.initials: C
authors.lastname: de Taisne
authors.forename: M C
authors.initials: MC
authors.lastname: Seguin
authors.forename: W I
authors.initials: WI
authors.lastname: Cox
authors.forename: J P
authors.initials: JP
authors.lastname: Winslow
authors.forename: L A
authors.initials: LA
authors.lastname: Ware
authors.forename: E B
authors.initials: EB
authors.lastname: Kauffman
authors.forename: D
authors.initials: D
authors.lastname: Gordon
authors.forename: W R
authors.initials: WR
authors.lastname: Ballou
authors.forename: E
authors.initials: E
authors.lastname: Paoletti
authors.forename: J C
authors.initials: JC
authors.lastname: Sadoff
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000096:biosynthesis; D006801:Humans; D007111:Immunity, Cellular; D008288:Malaria / Q000276:immunology / Q000517:prevention & control*; D051379:Mice; D008807:Mice, Inbred BALB C; D010962:Plasmodium berghei / Q000235:genetics / Q000276:immunology*; D015800:Protozoan Proteins / Q000235:genetics / Q000276:immunology*; D016052:Protozoan Vaccines / Q000235:genetics / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D016176:T-Lymphocyte Subsets / Q000276:immunology; D014613:Vaccines, Attenuated / Q000235:genetics / Q000494:pharmacology; D014614:Vaccines, Synthetic / Q000235:genetics / Q000494:pharmacology*; D014616:Vaccinia virus / Q000235:genetics / Q000276:immunology
chemical_list: D000913:Antibodies, Protozoan; D015800:Protozoan Proteins; D016052:Protozoan Vaccines; D011994:Recombinant Proteins; D014613:Vaccines, Attenuated; D014614:Vaccines, Synthetic; C041595:circumsporozoite protein, Protozoan