Genomic structure of the rice aldolase isozyme C-1 gene and its regulation through a Ca 2+ -mediated protein kinase-phosphatase pathway.
Complementary and genomic DNA clones coding for aldolase C-1, the fourth-type isozyme of aldolase in rice Oryza sativa L., have been characterized. The organization of the gene is quite similar to those encoding rice aldolase C-a and a maize cytoplasmic-type aldolase, in that introns are located in the same position. Amino acid sequences are highly conserved among cytoplasmic aldolases in plants. Expression of the gene in rice callus is activated by a protein phosphatase inhibitor okadaic acid, and is inhibited in the presence of thapsigargin, a reagent which increases calcium influx into the cytoplasm. The inhibition is rescued by the simultaneous addition of protein kinase inhibitor H-7. Thus, it is suggested that expression of the aldolase C-1 gene is regulated through a signal transduction pathway involving a Ca 2+ -mediated protein kinase-protein phosphatase system.
Nakamura
H
H
Institute for Cell Biology and Genetics, Faculty of Agriculture, Iwate University, Japan.
Satoh
W
W
Hidaka
S
S
Kagaya
Y
Y
Ejiri
S
S
Tsutsumi
K
K
D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D000595:Amino Acid Sequence; D001483:Base Sequence; D002118:Calcium / Q000378:metabolism; D000252:Calcium-Transporting ATPases / Q000037:antagonists & inhibitors; D004988:Ethers, Cyclic / Q000494:pharmacology; D005634:Fructose-Bisphosphate Aldolase / Q000235:genetics*; D015971:Gene Expression Regulation, Enzymologic; D017343:Genes, Plant; D007527:Isoenzymes / Q000235:genetics*; D007546:Isoquinolines / Q000494:pharmacology; D008969:Molecular Sequence Data; D019319:Okadaic Acid; D012275:Oryza / Q000201:enzymology / Q000235:genetics*; D010749:Phosphoprotein Phosphatases / Q000037:antagonists & inhibitors / Q000378:metabolism; D010879:Piperazines / Q000494:pharmacology; D047428:Protein Kinase Inhibitors; D011494:Protein Kinases / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction; D013729:Terpenes / Q000494:pharmacology; D019284:Thapsigargin
D004988:Ethers, Cyclic; D007527:Isoenzymes; D007546:Isoquinolines; D010879:Piperazines; D047428:Protein Kinase Inhibitors; D013729:Terpenes; D019319:Okadaic Acid; D019284:Thapsigargin; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D011494:Protein Kinases; D010749:Phosphoprotein Phosphatases; D005634:Fructose-Bisphosphate Aldolase; D000252:Calcium-Transporting ATPases; D002118:Calcium