Spontaneous granulocyte-macrophage colony growth by peripheral blood mononuclear cells in myeloproliferative disorders.
In the present study, the ability of peripheral blood (PB) progenitor cells to form granulocyte-macrophage (GM) colonies spontaneously in methylcellulose was investigated in healthy controls and patients with myeloproliferative disorders (MPDs). Spontaneous colony formation was observed in only one of the 18 control cases (6%), but in 22 of the 29 MPD patients (76%). The incidence of spontaneous GM colonies correlated both with the number of blast cells and the amount of c-kit positive cells present in the initial sample. Spontaneous GM colony growth in PB mononuclear cells isolated from patients with MPDs seems to be a frequent phenomenon in contrast to the healthy controls and may present a marker of malignancy.
Siitonen
T
T
Department of Internal Medicine, University of Oulu, Finland.
Zheng
A
A
Savolainen
E R
ER
Koistinen
P
P
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D018952:Antigens, CD34 / Q000378:metabolism; D002455:Cell Division; D003114:Colony-Forming Units Assay; D005260:Female; D006098:Granulocytes / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D006412:Hematopoietic Stem Cells / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D008264:Macrophages / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D009196:Myeloproliferative Disorders / Q000097:blood* / Q000276:immunology / Q000473:pathology; D019009:Proto-Oncogene Proteins c-kit / Q000378:metabolism
D018952:Antigens, CD34; D019009:Proto-Oncogene Proteins c-kit