title: Chronic ouabain infusion does not cause hypertension in sheep.
abstract: Ouabain is claimed to be a hormone of adrenal origin, capable of raising arterial pressure in rats. We infused ouabain in conscious sheep under carefully controlled circumstances to determine its effects on blood pressure, urine electrolytes, and vasoactive hormones. Eight healthy ewes were studied while taking a constant intake of dietary sodium and potassium. Ouabain infusion at 0.25 mg daily over 22 days reduced heart rate and arterial pressure and had no effect on pressor responsiveness to incremental intravenous infusions of angiotensin II. Ouabain induced minor, but statistically significant, decrements in urine volume, urinary sodium excretion, plasma renin and angiotensin II concentrations, and a rise in plasma aldosterone and cortisol. Plasma ouabain levels averaged 1.37 +/- 0.28 nmol/l during ouabain infusion. In conclusion, high-dose chronic ouabain infusion in sheep did not elevate arterial pressure or alter pressor responsiveness to angiotensin II, was antidiuretic and antinatriuretic, and induced minor perturbations in circulating renin, angiotensin II, aldosterone, and cortisol.
authors.affiliation: Department of Nephrology, Christchurch Hospital, New Zealand.
authors.forename: G B
authors.initials: GB
authors.lastname: Pidgeon
authors.forename: A M
authors.initials: AM
authors.lastname: Richards
authors.forename: M G
authors.initials: MG
authors.lastname: Nicholls
authors.forename: C J
authors.initials: CJ
authors.lastname: Charles
authors.forename: M T
authors.initials: MT
authors.lastname: Rademaker
authors.forename: K L
authors.initials: KL
authors.lastname: Lynn
authors.forename: R R
authors.initials: RR
authors.lastname: Bailey
authors.forename: L K
authors.initials: LK
authors.lastname: Lewis
authors.forename: T G
authors.initials: TG
authors.lastname: Yandle
mesh_terms: D000324:Adrenocorticotropic Hormone / Q000097:blood; D000450:Aldosterone / Q000097:blood; D000804:Angiotensin II / Q000097:blood / Q000494:pharmacology*; D000818:Animals; D009320:Atrial Natriuretic Factor / Q000097:blood; D001794:Blood Pressure / Q000187:drug effects*; D006152:Cyclic GMP / Q000097:blood; D004305:Dose-Response Relationship, Drug; D005260:Female; D006339:Heart Rate / Q000187:drug effects*; D006973:Hypertension / Q000139:chemically induced; D007262:Infusions, Intravenous; D010042:Ouabain / Q000008:administration & dosage / Q000097:blood / Q000494:pharmacology*; D011188:Potassium / Q000652:urine; D011674:Pulse / Q000187:drug effects; D051381:Rats; D012083:Renin / Q000097:blood; D012084:Renin-Angiotensin System / Q000187:drug effects; D012756:Sheep; D012964:Sodium / Q000652:urine; D013997:Time Factors
chemical_list: D000804:Angiotensin II; D000450:Aldosterone; D010042:Ouabain; D009320:Atrial Natriuretic Factor; D000324:Adrenocorticotropic Hormone; D012964:Sodium; D012083:Renin; D006152:Cyclic GMP; D011188:Potassium