Completion of the amino acid sequence of the C-terminal half of the porcine estradiol receptor by Edman degradation: reconfirmation of the absence of O-linked sugars and phosphates.
The peptide A569-Y582 of the porcine estradiol receptor containing the missing sequence T570-M581 (1) was isolated and sequenced. The 4 seryl- and 2 threonyl-PTH amino acids were recovered in normal yields, excluding their posttranslational modification and reconfirming the absence of O-glycosylation and O-phosphorylation in H267-I595.
Thole
H H
HH
Max-Planck-institut für Experimentelle Endokrinologie, Hannover, Germany.
Jungblut
P W
PW
D000595:Amino Acid Sequence; D000818:Animals; D002241:Carbohydrates / Q000032:analysis; D002846:Chromatography, Affinity; D005260:Female; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry* / Q000302:isolation & purification; D010449:Peptide Mapping; D010710:Phosphates / Q000032:analysis; D011959:Receptors, Estradiol / Q000737:chemistry*; D012741:Sexual Maturation; D013552:Swine; D014599:Uterus / Q000737:chemistry
D002241:Carbohydrates; D010446:Peptide Fragments; D010710:Phosphates; D011959:Receptors, Estradiol