title: Immunoprophylaxis of allergen-induced immunoglobulin E synthesis and airway hyperresponsiveness in vivo by genetic immunization.
abstract: The efficacy of an "allergen-gene immunization" protocol in altering allergic response was examined. Intramuscular injection of rats with a plasmid DNA encoding a house dust mite allergen into the muscle results in its long-term expression and the induction of specific immune responses. Significantly, this approach prevents the induction of immunoglobulin E synthesis, histamine release in bronchoalveolar fluids, and airway hyperresponsiveness in rats challenged with aerosolized allergen. Furthermore, this suppression is persistent and can be transferred into naive rats by CD8+ T cells from gene-immunized rats. These findings suggest that allergen-gene immunization is effective in modulating allergic responses, and may provide a novel therapeutic approach for allergic diseases.
authors.affiliation: Graduate Institute of Microbiology, College of Medicine, National Taiwan University, Taipei, Republic of China.
authors.forename: C H
authors.initials: CH
authors.lastname: Hsu
authors.forename: K Y
authors.initials: KY
authors.lastname: Chua
authors.forename: M H
authors.initials: MH
authors.lastname: Tao
authors.forename: Y L
authors.initials: YL
authors.lastname: Lai
authors.forename: H D
authors.initials: HD
authors.lastname: Wu
authors.forename: S K
authors.initials: SK
authors.lastname: Huang
authors.forename: K H
authors.initials: KH
authors.lastname: Hsieh
mesh_terms: D000818:Animals; D039741:Antigens, Dermatophagoides; D001992:Bronchoalveolar Lavage Fluid; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology / Q000637:transplantation; D004274:DNA, Recombinant / Q000627:therapeutic use*; D006023:Glycoproteins / Q000235:genetics / Q000276:immunology / Q000627:therapeutic use*; D006632:Histamine / Q000378:metabolism; D007073:Immunoglobulin E / Q000096:biosynthesis*; D007150:Immunohistochemistry; D008297:Male; D051381:Rats; D012130:Respiratory Hypersensitivity / Q000276:immunology / Q000517:prevention & control*; D014611:Vaccination
chemical_list: D039741:Antigens, Dermatophagoides; D004274:DNA, Recombinant; D006023:Glycoproteins; D007073:Immunoglobulin E; D006632:Histamine