Chemokines and T lymphocyte recruitment to lymph nodes in HIV infection.
Recruitment of T lymphocytes to lymph nodes in patients with HIV infection is critical to the pathogenesis of disease. Chemokines are a family of cytokines, which are potent regulators of leukocyte migration. We studied the leukocyte populations and expression of chemokines known to be active upon T cells in lymph nodes of four HIV infected patients and seven control subjects using in situ hybridization, immunohistochemistry, and FACS analysis. The HIV lymph nodes showed CD8+ T lymphocyte accumulation and strongly enhanced chemokine expression, notably for the CD8+ T cell chemoattractant, macrophage inflammatory protein (MIP)-1 alpha. Resident macrophages appeared to be a major cellular source of chemokines in the HIV nodes. RANTES expression was present in both HIV and control lymph nodes, suggesting a physiological role for this chemokine in T lymphocyte recirculation. Chemokines may be important determinants of T lymphocyte accumulation in lymphoid tissue of patients with HIV/AIDS.
Tedla
N
N
Inflammation Research Unit, School of Pathology, University of New South Wales, Sydney, Australia.
Palladinetti
P
P
Kelly
M
M
Kumar
R K
RK
DiGirolamo
N
N
Chattophadhay
U
U
Cooke
B
B
Truskett
P
P
Dwyer
J
J
Wakefield
D
D
Lloyd
A
A
D000328:Adult; D000368:Aged; D016827:CD8 Antigens / Q000032:analysis; D002465:Cell Movement / Q000502:physiology; D054407:Chemokine CCL4; D018946:Chemokine CCL5 / Q000378:metabolism / Q000502:physiology; D018925:Chemokines / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D005434:Flow Cytometry; D015658:HIV Infections / Q000209:etiology / Q000276:immunology / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008198:Lymph Nodes / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D019402:Macrophage Inflammatory Proteins; D008297:Male; D008875:Middle Aged; D015846:Monokines / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism / Q000473:pathology*
D016827:CD8 Antigens; D054407:Chemokine CCL4; D018946:Chemokine CCL5; D018925:Chemokines; D019402:Macrophage Inflammatory Proteins; D015846:Monokines; D012333:RNA, Messenger