title: Rab11 is an apically located small GTP-binding protein in epithelial tissues.
abstract: Rab proteins are involved in many aspects of dynamic vesicle processing within eukaryotic cells. We have previously identified Rab11 in gastric parietal cell tubulovesicle membranes. We have produced a monoclonal antibody that is specific for Rab11. In all rabbit tissues examined, Rab11 immunoreactivity was highly enriched in epithelial cells. In the gastric fundus, parietal cells were stained in a pattern consistent with localization on tubulovesicles. Surface mucous cells of both the fundus and antrum demonstrated punctate subapical staining. Ileal and proximal colonic enterocyte labeling was observed deep to the brush borders. In the distal colon, staining was observed in the apical regions of mid-crypt cells. In skin and esophagus, punctate immunoreactivity was present in the medial layers of the squamous epithelia. Prominent Rab11 immunoreactivity was present in hepatocytes deep to the bile canaliculi. Punctate subapical staining was observed in pancreatic acinar cells. Apical staining was also observed in collecting duct cells and in the glandular cells of the prostate. These results indicate the Rab11 is expressed in apical vesicular populations in discrete epithelial cell populations.
authors.affiliation: Department of Medicine, Medical College of Georgia, Augusta 30912, USA.
authors.forename: J R
authors.initials: JR
authors.lastname: Goldenring
authors.forename: J
authors.initials: J
authors.lastname: Smith
authors.forename: H D
authors.initials: HD
authors.lastname: Vaughan
authors.forename: P
authors.initials: P
authors.lastname: Cameron
authors.forename: W
authors.initials: W
authors.lastname: Hawkins
authors.forename: J
authors.initials: J
authors.lastname: Navarre
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D001483:Base Sequence; D015153:Blotting, Western; D003106:Colon; D017931:DNA Primers; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism; D004947:Esophagus / Q000166:cytology; D019204:GTP-Binding Proteins / Q000032:analysis* / Q000096:biosynthesis*; D005748:Gastric Fundus; D005753:Gastric Mucosa / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000166:cytology* / Q000378:metabolism; D007668:Kidney / Q000166:cytology; D008099:Liver / Q000166:cytology; D008969:Molecular Sequence Data; D009092:Mucous Membrane / Q000166:cytology; D016376:Oligonucleotides, Antisense; D009928:Organ Specificity; D010179:Pancreas / Q000166:cytology; D010295:Parietal Cells, Gastric / Q000166:cytology* / Q000378:metabolism; D011706:Pyloric Antrum; D011817:Rabbits; D011994:Recombinant Proteins / Q000096:biosynthesis; D017386:Sequence Homology, Amino Acid; D012867:Skin / Q000166:cytology; D020691:rab GTP-Binding Proteins; D000099193:rab11 GTP-Binding Proteins
chemical_list: D000911:Antibodies, Monoclonal; D017931:DNA Primers; D019204:GTP-Binding Proteins; D016376:Oligonucleotides, Antisense; D011994:Recombinant Proteins; D020691:rab GTP-Binding Proteins; D000099193:rab11 GTP-Binding Proteins