title: Histidine patch thioredoxins. Mutant forms of thioredoxin with metal chelating affinity that provide for convenient purifications of thioredoxin fusion proteins.
abstract: A cluster of surface amino acid residues on Escherichia coli thioredoxin were systematically mutated in order to provide the molecule with an ability to chelate metal ions. The combined effect of two histidine mutants, E30H and Q62H, gave thioredoxin the capacity to bind to nickel ions immobilized on iminodiacetic acid- and nitrilotriacetic acid-Sepharose resins. Even though these two histidines were more than 30 residues apart in thioredoxin's primary sequence, they were found to satisfy the geometric constraints for metal ion coordination as a result of the thioredoxin tertiary fold. A third histidine mutation, S1H, provided additional metal ion chelation affinity, but the native histidine at position 6 of thioredoxin was found not to participate in binding. All of the histidine mutants exhibited decreased thermal stability as compared with wild-type thioredoxin; however, the introduction of an additional mutation, D26A, increased their melting temperatures beyond that of wild-type thioredoxin. The metal chelating abilities of these histidine mutants of thioredoxin were successfully utilized for convenient purifications of human interleukin-8 and -11 expressed in E. coli as soluble thioredoxin fusion proteins.
authors.affiliation: Genetics Institute, Inc., Cambridge, Massachusetts 02140, USA.
authors.forename: Z
authors.initials: Z
authors.lastname: Lu
authors.forename: E A
authors.initials: EA
authors.lastname: DiBlasio-Smith
authors.forename: K L
authors.initials: KL
authors.lastname: Grant
authors.forename: N W
authors.initials: NW
authors.lastname: Warne
authors.forename: E R
authors.initials: ER
authors.lastname: LaVallie
authors.forename: L A
authors.initials: LA
authors.lastname: Collins-Racie
authors.forename: M T
authors.initials: MT
authors.lastname: Follettie
authors.forename: M J
authors.initials: MJ
authors.lastname: Williamson
authors.forename: J M
authors.initials: JM
authors.lastname: McCoy
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D002614:Chelating Agents; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003001:Cloning, Molecular; D003198:Computer Simulation; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000378:metabolism*; D006639:Histidine; D006801:Humans; D008958:Models, Molecular; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D017354:Point Mutation; D017510:Protein Folding; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D013879:Thioredoxins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D002614:Chelating Agents; D011993:Recombinant Fusion Proteins; D006639:Histidine; D013879:Thioredoxins