ET-Kyoto solution for 48-hour canine lung preservation.
BACKGROUND
ET-Kyoto (ET-K) solution, proven safe for 20-hour lung preservation, was modified to achieve longer preservation: ET-K2 solution with more buffer capacity and ET-K3 with less potassium.


METHODS
Lungs were preserved with one of the three solutions (with prostaglandin E1 at 4 degrees C for 48 hours (n = 5 for each). Left lung transplantation was performed and evaluated for 6 hours.


RESULTS
Each solution became acidic after preservation (p < 0.01), though the change was lowest in the ET-K2 solution. All animals in the ET-K and ET-K3 groups survived for 6 hours after reperfusion, but only 1 survived in the ET-K2 group (p < 0.05). In all groups, partial pressure of oxygen in arterial blood decreased gradually after reperfusion. Pulmonary vascular resistance after reperfusion was significantly lower in the ET-K group than in the ET-K3 group (p < 0.01). Scanning electron microscopic examination showed that endothelial cell swelling and disruption were milder in the ET-K group (with the solution containing potassium of 44 mEq/L) than in the ET-K3 group.


CONCLUSIONS
Lung preservation can be achieved for 48 hours in ET-K and ET-K3 solutions. Enhancement of buffer capacity provides no advantage. Potassium at 44 mEq/L does not cause deterioration of endothelial cells.
Wada
H
H
Department of Thoracic Surgery, Chest Disease Research Institute, Kyoto university, Japan.
Fukuse
T
T
Nakamura
T
T
Liu
C J
CJ
Bando
T
T
Kosaka
S
S
Ariyasu
T
T
Hitomi
S
S
D000818:Animals; D004285:Dogs; D005942:Gluconates / Q000627:therapeutic use*; D006895:Hydroxyethyl Starch Derivatives / Q000627:therapeutic use*; D008168:Lung / Q000473:pathology; D009926:Organ Preservation; D010710:Phosphates; D013997:Time Factors; D014199:Trehalose / Q000627:therapeutic use*
C087059:ET-Kyoto solution; D005942:Gluconates; D006895:Hydroxyethyl Starch Derivatives; D010710:Phosphates; D014199:Trehalose