title: Karyotype conversion in two patients with chronic myeloid leukaemia after busulphan-induced marrow hypoplasia.
abstract: We report two patients with chronic myeloid leukaemia (CML) developing hypoplasia and karyotype conversion after conventional busulphan therapy. Initially, the percentage of Ph-positive metaphases in marrow for both patients was 100%, which steadily diminished up to a complete disappearance in case 1 and decreased dramatically in case 2 following hypoplasia. Thereafter Southern blot and RT-PCR assays revealed no abnormalities. Both patients have survived 9 years and remained in good clinical and haematological remission without any treatment until recently. We believe that the high sensitivity to busulphan therapy result in hypoplasia and karyotype conversion, which contributed to prolonged survival.
authors.affiliation: Leukaemia Research Unit, Jiangsu Institute of Haematology, Suzhou, P. R. China.
authors.forename: Y
authors.initials: Y
authors.lastname: Xue
authors.forename: X J
authors.initials: XJ
authors.lastname: Zhou
authors.forename: F
authors.initials: F
authors.lastname: Yu
authors.forename: J
authors.initials: J
authors.lastname: Gu
authors.forename: Y
authors.initials: Y
authors.lastname: Guo
authors.forename: X
authors.initials: X
authors.lastname: Xie
authors.forename: B
authors.initials: B
authors.lastname: Lin
mesh_terms: D000328:Adult; D018906:Antineoplastic Agents, Alkylating / Q000009:adverse effects*; D001483:Base Sequence; D015139:Blotting, Southern; D001855:Bone Marrow Diseases / Q000139:chemically induced*; D002066:Busulfan / Q000009:adverse effects*; D002881:Chromosomes, Human, Pair 12; D002886:Chromosomes, Human, Pair 17; D002892:Chromosomes, Human, Pair 22; D002899:Chromosomes, Human, Pair 9; D006801:Humans; D007621:Karyotyping; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000188:drug therapy* / Q000235:genetics; D008297:Male; D008969:Molecular Sequence Data; D014178:Translocation, Genetic
chemical_list: D018906:Antineoplastic Agents, Alkylating; D002066:Busulfan