Isolation and characterization of Azospirillum lipoferum locus that complements Rhizobium meliloti dctA and dctB mutations.
A DNA probe containing the structural gene for dicarboxylate transport (dct A) of Rhizobium meliloti hybridized strongly with the fragments of Azospirillum lipoferum genomic DNA. A genomic library of A. lipoferum was screened for the dct A gene by complementation of a dct A mutant of Rhizobium meliloti. A recombinant cosmid, p37D, capable of restoring growth of the dct A mutant on dicarboxylates was isolated and found to hybridize to the dctA probe. The ability of p37D to complement the dct B mutant of R. meloliti indicated that dct A and dct B genes in A. lipoferum may be organized adjacent to each other.
Tripathi
A K
AK
School of Biotechnology, Faculty of Science, Banaras Hindu University, Varanasi, India.
Mishra
B M
BM
D016949:Azospirillum / Q000235:genetics*; D001426:Bacterial Proteins; D001693:Biological Transport, Active / Q000235:genetics; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D003001:Cloning, Molecular; D003360:Cosmids; D015342:DNA Probes; D004269:DNA, Bacterial / Q000235:genetics; D029382:Dicarboxylic Acid Transporters; D003998:Dicarboxylic Acids / Q000378:metabolism; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D005810:Multigene Family; D009154:Mutation; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism; D015183:Restriction Mapping; D016962:Sinorhizobium meliloti / Q000235:genetics* / Q000378:metabolism
D001426:Bacterial Proteins; D002352:Carrier Proteins; D015342:DNA Probes; D004269:DNA, Bacterial; D029382:Dicarboxylic Acid Transporters; D003998:Dicarboxylic Acids; D011956:Receptors, Cell Surface; C073786:dctA protein, Sinorhizobium meliloti; C073787:DCTB protein, Sinorhizobium; D011494:Protein Kinases