Isolation of Rhodobacter capsulatus transketolase: cloning and sequencing of its structural tktA gene.
Rhodobacter capsulatus transketolase (Tkt) protein has been isolated from strain B10 by heparin affinity chromatography. Oligodeoxyribonucleotides (oligo) constructed as based on the amino-acid sequences were used for polymerase chain reaction (PCR) amplification on total genomic DNA. Southern hybridization with the PCR product as a probe allowed the isolation of a 5-kb PstI DNA fragment containing the structural Tkt-encoding gene (tktA) which was cloned and sequenced. The deduced tktA product of 671 aa (72815 Da) shares 59% identity with Rhodobacter sphaeroides Tkt.
de Sury d'Aspremont
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R
CEA Grenoble, Biochimie Microbienne, Département de Biologie Moléculaire et Structurale, France.
Toussaint
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B
Vignais
P M
PM
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000302:isolation & purification; D016354:Rhodobacter capsulatus / Q000235:genetics*; D014174:Transketolase / Q000235:genetics*
D011994:Recombinant Proteins; D014174:Transketolase