Translational regulation in vivo of the Drosophila melanogaster mRNA encoding succinate dehydrogenase iron protein via iron responsive elements.
Some mRNA encoding proteins related to iron metabolism contain a specific stem-loop structure--iron responsive element (IRE)-in the 5' UTR. Binding of the iron regulatory protein (IRP) to the IRE, in response to decreases in cellular iron levels, leads to a block in translation of these mRNAs. We here describe the drosophila melanogaster succinate dehydrogenase iron protein (SDH-IP) as a fourth example of an mRNA species being translationally regulated by an IRE, based on iron dependent regulation of SDH-IP translation in vivo by immunoprecipitations and northern blotting in drosophila cell lines. Addition of hemin to the drosophila cells lead to fragmentation of the SDH-IP, which might suggest additional mode to specifically down-regulate the expression of this protein and Krebs cycle function.
Melefors
O
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Department of Cell and Molecular Biology, Karolinska Insitute, Stockholm, Sweden.
D000818:Animals; D001483:Base Sequence; D002952:Citric Acid Cycle; D004331:Drosophila melanogaster / Q000235:genetics*; D006801:Humans; D007501:Iron / Q000378:metabolism*; D035925:Iron-Regulatory Proteins; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics*; D016601:RNA-Binding Proteins / Q000378:metabolism*; D013385:Succinate Dehydrogenase / Q000235:genetics*
D035925:Iron-Regulatory Proteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D007501:Iron; D013385:Succinate Dehydrogenase