Molecular cloning of cDNA and analysis of expression of the gene for alpha-glucosidase from the hypopharyngeal gland of the honeybee Apis mellifera L.
Previously, we identified and purified an alpha-glucosidase with a molecular mass of 70 kDa from a homogenate of the hypopharyngeal gland of the older worker bee (forager bee) Apis mellifera L. (T. Kubo et al, J. Biochem., 1996, 119, 291-295). Here, we isolated and sequenced a cDNA for the alpha-glucosidase. The cDNA encoded a protein consisting of 650 amino acids, which had high sequence identity with fruit fly and mosquito possible maltase gene products. RT-PCR showed that the gene was expressed specifically in the hypopharyngeal gland of the forager bee.
Ohashi
K
K
Faculty of Pharmaceutical Sciences, University of Tokyo, Japan.
Sawata
M
M
Takeuchi
H
H
Natori
S
S
Kubo
T
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001516:Bees; D003001:Cloning, Molecular; D018076:DNA, Complementary; D015971:Gene Expression Regulation, Enzymologic; D008969:Molecular Sequence Data; D010614:Pharynx / Q000201:enzymology*; D017386:Sequence Homology, Amino Acid; D000520:alpha-Glucosidases / Q000235:genetics*
D018076:DNA, Complementary; D000520:alpha-Glucosidases