title: Analysis of 0.5-kilobase-pair repeats in the Mycoplasma hominis lmp gene system and identification of gene products.
abstract: Mycoplasma hominis, an opportunistic pathogenic bacterium of humans, has a small genome of 700 kb. Despite this, multiple copies of gene sequences with similarities to the structural gene (lmp1) of a 135-kDa surface-located membrane protein (Lmp1) have been identified on the genome of M. hominis PG21 (lmp2, lmp3, and lmp4). The distance between the lmp1-lmp2 region and the lmp3-lmp4 region was more than 110 kb. lmp3-lmp4 of M. hominis PG21 was sequenced and found to contain two putative genes. The gene region of 6.5 kb contained a 5' unique region and a 3' unique region separated by 9 0.5-kb repeats with 51 to 90% similarity to 10 similar repeats found in the lmp1-lmp2 region. The 0.5-kb DNA repeats thus comprised about 1% of the entire genome. In both regions, a base change in one of the repeats gave rise to a stop codon, and thereby lmp2 and lmp4 occurred. By PCR amplification of reverse-transcriptase-generated cDNA it was shown that all four genes were transcribed. By use of Lmp-specific antibodies we showed that both lmp1 and lmp3 were translated into proteins (Lmp1 and Lmp3). Each of the four lmp genes represented by their unique cloned segments was used as a probe to analyze the presence, distribution, and organization of the genes within the genome in 13 M. hominis isolates. The repetitive element was detected at one or two locations on the chromosome for all isolates. The lmp3-specific element was present in all isolates, and lmp1- and lmp2-specific elements were present in all but one isolate. The lmp4-specific element was present in about half the isolates tested. For five M. hominis isolates the chromosomal location of the lmp genes was mapped.
authors.affiliation: University of Aarhus, Aarhus, Denmark. sorenl@biobase.dk
authors.forename: S A
authors.initials: SA
authors.lastname: Ladefoged
authors.forename: L T
authors.initials: LT
authors.lastname: Jensen
authors.forename: B
authors.initials: B
authors.lastname: Brock
authors.forename: S
authors.initials: S
authors.lastname: Birkelund
authors.forename: G
authors.initials: G
authors.lastname: Christiansen
mesh_terms: D000595:Amino Acid Sequence; D000907:Antibodies, Bacterial; D001426:Bacterial Proteins / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D001483:Base Sequence; D017124:Conserved Sequence; D005798:Genes, Bacterial; D006801:Humans; D008969:Molecular Sequence Data; D009174:Mycoplasma / Q000145:classification / Q000235:genetics*; D009175:Mycoplasma Infections / Q000382:microbiology; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D014158:Transcription, Genetic
chemical_list: D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; C099481:Lmp3 protein, Mycoplasma; C099482:Lmp4 protein, Mycoplasma