Expression of Hel-N1 and Hel-N2 in small-cell lung carcinoma.
Hel-N1 and HuD are neuron-specific RNA-binding proteins that are antigenic targets of anti-Hu antibodies. Although expression of Hu antigens is most commonly seen in small-cell lung carcinoma, their exact identity (e.g., Hel-NI, Hel-N2, HuD, and HuC cannot be distinguished by immunological methods. Analysis of messenger RNA expression is needed for this distinction. Here we demonstrate that Hel-NI and Hel-N2 are expressed in small-cell lung carcinoma using reverse transcription-polymerase chain reaction.
King
P H
PH
Department of Neurology, The University of Alabama, Birmingham, 35294-0007, USA.
Dropcho
E J
EJ
D001483:Base Sequence; D018288:Carcinoma, Small Cell / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D051959:ELAV Proteins; D000067777:ELAV-Like Protein 2; D000067779:ELAV-Like Protein 4; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology; D006801:Humans; D007536:Isomerism; D004915:Leukemia, Erythroblastic, Acute; D008175:Lung Neoplasms / Q000235:genetics*; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics*; D016133:Polymerase Chain Reaction; D016601:RNA-Binding Proteins / Q000235:genetics*; D014407:Tumor Cells, Cultured / Q000502:physiology
D017931:DNA Primers; D051959:ELAV Proteins; D000067777:ELAV-Like Protein 2; D000067779:ELAV-Like Protein 4; C086811:ELAVL2 protein, human; C498010:ELAVL4 protein, human; D009419:Nerve Tissue Proteins; D016601:RNA-Binding Proteins