title: Determination of cyclophosphamide metabolites by gas chromatography and thermionic specific detection. Interindividual differences in hepatic biotransformation of cyclophosphamide in man in vitro.
abstract: Sensitive methods for the determination of the cyclophosphamide metabolites nornitrogen mustard, 4-ketocyclophosphamide and carboxyphosphamide are presented. After liquid-liquid extraction and derivatization, the metabolites are determined by gas chromatography and thermionic specific detection. The methods were used to study the in vitro biotransformation of cyclophosphamide with S-9 liver fractions of human donors. The results show large interindividual differences in the formation of nornitrogen mustard and carboxyphosphamide. 4-Ketocyclophosphamide was not detected.
authors.affiliation: Department of Toxicology, Faculty of Medical Sciences, University of Nijmegen, Netherlands.
authors.forename: P J
authors.initials: PJ
authors.lastname: Sessink
authors.forename: W H
authors.initials: WH
authors.lastname: Vaes
authors.forename: P H
authors.initials: PH
authors.lastname: van den Broek
authors.forename: J
authors.initials: J
authors.lastname: Noordhoek
authors.forename: R P
authors.initials: RP
authors.lastname: Bos
mesh_terms: D002849:Chromatography, Gas / Q000379:methods* / Q000706:statistics & numerical data; D003520:Cyclophosphamide / Q000032:analysis / Q000378:metabolism* / Q000493:pharmacokinetics*; D006801:Humans; D008099:Liver / Q000737:chemistry / Q000378:metabolism*; D009584:Nitrogen / Q000032:analysis; D010752:Phosphoramide Mustards / Q000032:analysis
chemical_list: D010752:Phosphoramide Mustards; D003520:Cyclophosphamide; C000028:carboxyphosphamide; D009584:Nitrogen