Inhibition of 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) mutagenicity by black and green tea extracts and polyphenols.
Solutions of lyophilized preparations of standard black and green tea extracts were made and tested over a range of six concentrations as inhibitors of the mutagenicity caused by the fool mutagen 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) in the Salmonella typhimurium TA98 assay containing S9 fraction from rats induced with alpha-naphthoflavone and phenobarbital. Extracts of both black and green tea were equally good inhibitors of mutagenicity. Purified polyphenols were prepared from tea extracts by solvent extraction. The polyphenols of black tea were more potent inhibitors of mutagenicity than the polyphenols of green tea. These findings suggest that black tea may have similar health-promoting properties to those reported previously for green tea.
Apostolides
Z
Z
Department of Biochemistry, University of Pretoria, South Africa.
Balentine
D A
DA
Harbowy
M E
ME
Weisburger
J H
JH
D000818:Animals; D016587:Antimutagenic Agents / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005419:Flavonoids; D007093:Imidazoles / Q000037:antagonists & inhibitors*; D008297:Male; D009153:Mutagens; D010636:Phenols / Q000494:pharmacology*; D010936:Plant Extracts / Q000494:pharmacology; D011108:Polymers / Q000494:pharmacology*; D059808:Polyphenols; D051381:Rats; D017207:Rats, Sprague-Dawley; D013662:Tea
D016587:Antimutagenic Agents; D005419:Flavonoids; D007093:Imidazoles; D009153:Mutagens; D010636:Phenols; D010936:Plant Extracts; D011108:Polymers; D059808:Polyphenols; D013662:Tea; C049584:2-amino-1-methyl-6-phenylimidazo(4,5-b)pyridine