title: Inhibition of the association of RNA polymerase II with the preinitiation complex by a viral transcriptional repressor.
abstract: Transcriptional repression is an important component of regulatory networks that govern gene expression. In this report, we have characterized the mechanisms by which the immediate early protein 2 (IE2 or IE86), a master transcriptional regulator of human cytomegalovirus, down-regulates its own expression. In vitro transcription and DNA binding experiments demonstrate that IE2 blocks specifically the association of RNA polymerase II with the preinitiation complex. Although, to our knowledge, this is the first report to describe a eukaryotic transcriptional repressor that selectively impedes RNA polymerase II recruitment, we present data that suggest that this type of repression might be widely used in the control of transcription by RNA polymerase II.
authors.affiliation: Tularik Inc., South San Francisco, California 94080, USA.
authors.forename: G
authors.initials: G
authors.lastname: Lee
authors.forename: J
authors.initials: J
authors.lastname: Wu
authors.forename: P
authors.initials: P
authors.lastname: Luu
authors.forename: P
authors.initials: P
authors.lastname: Ghazal
authors.forename: O
authors.initials: O
authors.lastname: Flores
mesh_terms: D001483:Base Sequence; D001667:Binding, Competitive; D003587:Cytomegalovirus / Q000235:genetics; D004268:DNA-Binding Proteins / Q000378:metabolism; D015967:Gene Expression Regulation, Viral; D017874:Immediate-Early Proteins / Q000378:metabolism*; D046911:Macromolecular Substances; D008562:Membrane Glycoproteins; D008969:Molecular Sequence Data; D012319:RNA Polymerase II / Q000037:antagonists & inhibitors / Q000378:metabolism*; D012097:Repressor Proteins / Q000378:metabolism*; D015534:Trans-Activators; D035362:Transcription Factor TFIID; D014157:Transcription Factors / Q000378:metabolism; D014158:Transcription, Genetic; D014759:Viral Envelope Proteins; D014764:Viral Proteins
chemical_list: D004268:DNA-Binding Proteins; C069186:IE2 protein, Cytomegalovirus; D017874:Immediate-Early Proteins; D046911:Macromolecular Substances; D008562:Membrane Glycoproteins; D012097:Repressor Proteins; D015534:Trans-Activators; D035362:Transcription Factor TFIID; D014157:Transcription Factors; C077406:UL115 protein, Human herpesvirus 5; D014759:Viral Envelope Proteins; D014764:Viral Proteins; C071440:glycoprotein H, Cytomegalovirus; C499358:glycoprotein H, Human cytomegalovirus; C439068:glycoprotein O, cytomegalovirus; D012319:RNA Polymerase II