title: Cloning of a potential cytochrome P450 from the archaeon Sulfolobus solfataricus.
abstract: Abstract A gene, CYP119, for a potential cytochrome P450 has been isolated and sequenced from the extreme acidothermophilic archaeon Sulfolobus solfataricus. The gene predicts a polypeptide of 368 amino acids containing the consensus heme-binding sequence Phe-Gly-Xaa-Gly-Xaa-His-Xaa-Cys-Xaa-Gly- Xaa3-Ala-Arg-Xaa-Glu. It most closely resembles the cytochrome P450s found in the bacterium Bacillus subtilis, with which it shares 129 identical amino acid residues (35%). This first sequence of a potential archaeal cytochrome P450 represents an important step in tracing the complex evolutionary history of this biologically important enzyme family.
authors.affiliation: Department of Biochemistry and Anaerobic Microbiology, Virginia Polytechnic Institute and State University, Blacksburg 24061-0308, USA.
authors.forename: R L
authors.initials: RL
authors.lastname: Wright
authors.forename: K
authors.initials: K
authors.lastname: Harris
authors.forename: B
authors.initials: B
authors.lastname: Solow
authors.forename: R H
authors.initials: RH
authors.lastname: White
authors.forename: P J
authors.initials: PJ
authors.lastname: Kennelly
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D017124:Conserved Sequence; D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry / Q000235:genetics*; D019143:Evolution, Molecular; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D016944:Sulfolobus / Q000201:enzymology* / Q000235:genetics
chemical_list: D003577:Cytochrome P-450 Enzyme System