Effect of symplasmic isolation and antigibberellin treatment on morphogenesis in Chara.
The effects of plasmolytically induced symplasmic isolation and AMO-1618 treatment on the development of antheridia of Chara vulgaris L. were compared. After 24 h both factors inhibited endoreplication in manubria. In the youngest antheridia the inhibition of 3H-thymidine incorporation following plasmolysis was irreversible while the same effect caused by AMO-1618 disappeared after 3 days. Four-five days after plasmolysis or five days after AMO-1618 treatment antheridia which were at the stage of spermiogenesis had a lower level of DNA in manubria and revealed decreased productivity due to the smaller number of mitotic divisions in antheridial filaments. On the basis of the previous and present data a hypothesis was put forward that in both experiments premature spermiogenesis was caused by a rapid decrease in gibberellin level in the antheridia.
Kwiatkowska
M
M
Department of Cytophysiology, University of Lódź, Poland.
D002250:Carbon Radioisotopes; D000460:Chlorophyta / Q000166:cytology / Q000502:physiology* / Q000648:ultrastructure; D005875:Gibberellins / Q000037:antagonists & inhibitors*; D009024:Morphogenesis / Q000187:drug effects; D010937:Plant Growth Regulators / Q000494:pharmacology; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology; D013936:Thymidine / Q000378:metabolism; D013997:Time Factors; D014316:Tritium
D002250:Carbon Radioisotopes; D005875:Gibberellins; D010937:Plant Growth Regulators; D000644:Quaternary Ammonium Compounds; D014316:Tritium; C006155:AMO-1618; D013936:Thymidine