Oscillations in the peroxidase-oxidase reaction: a comparison of different peroxidases.
The nonlinear behavior of the peroxidase-oxidase reaction was studied using structurally different peroxidases. For the first time sustained oscillations with peroxidases other than horseradish peroxidase in a single-enzyme system were observed. All peroxidases that showed significant oxidase activity were able to generate sustained oscillations. When adjusting the overall reaction rate, either of the two modifiers 2,4-dichlorophenol or Methylene blue could be omitted from the reaction. Due to the observation of different enzyme intermediates when using different peroxidases, we conclude that the mechanisms responsible for oscillatory kinetics may vary from one peroxidase to the other.
Kummer
U
U
Institute for Chemical Plant Physiology, University of Tübingen, Germany. ursula.kummer@uni-tuebingen.de
Valeur
K R
KR
Baier
G
G
Wegmann
K
K
Olsen
L F
LF
D000818:Animals; D002417:Cattle; D002711:Chloride Peroxidase / Q000378:metabolism; D002733:Chlorophenols; D003302:Coprinus / Q000201:enzymology; D006735:Horseradish Peroxidase / Q000378:metabolism; D066298:In Vitro Techniques; D007700:Kinetics; D007784:Lactoperoxidase / Q000378:metabolism; D008751:Methylene Blue; D008892:Milk / Q000201:enzymology; D003904:Mitosporic Fungi / Q000201:enzymology; D009991:Oscillometry / Q000295:instrumentation; D010088:Oxidoreductases / Q000378:metabolism*; D010544:Peroxidases / Q000378:metabolism*; D013025:Glycine max / Q000201:enzymology
D002733:Chlorophenols; D010088:Oxidoreductases; D006735:Horseradish Peroxidase; D007784:Lactoperoxidase; D010544:Peroxidases; C021799:microperoxidase; D002711:Chloride Peroxidase; C004762:2,4-dichlorophenol; D008751:Methylene Blue