Glyceraldehyde-3-phosphate dehydrogenase is over-expressed during apoptotic death of neuronal cultures and is recognized by a monoclonal antibody against amyloid plaques from Alzheimer's brain.
The age-induced apoptotic death of cerebellar neurons in culture is associated with over-expression of a 38-kDa particulate protein identified as glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Both the age-induced apoptosis and the 38-kDa protein overexpression were effectively suppressed by the presence of tetrahydroaminoacridine, an antidementia drug, or aurintricarboxylic acid. This over-expressed 38-kDa protein and purified GAPDH were found to react with a monoclonal antibody (mAb), Am-3, which was raised against amyloid plaques from Alzheimer's brain, but not with mAb, AmT-1, which was produced using synthetic amyloid beta peptide. These results raise the possibility that GAPDH is also involved in the neurodegeneration during the development of Alzheimer's disease.
Sunaga
K
K
Group on Cellular Neurobiology, Josai University, Saitama, Japan.
Takahashi
H
H
Chuang
D M
DM
Ishitani
R
R
D000544:Alzheimer Disease / Q000276:immunology; D000682:Amyloid / Q000276:immunology*; D000818:Animals; D000911:Antibodies, Monoclonal; D017209:Apoptosis / Q000502:physiology*; D015153:Blotting, Western; D002478:Cells, Cultured / Q000737:chemistry / Q000166:cytology; D002531:Cerebellum / Q000166:cytology; D003470:Culture Media; D004591:Electrophoresis, Polyacrylamide Gel; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000096:biosynthesis* / Q000276:immunology / Q000378:metabolism; D008970:Molecular Weight; D009474:Neurons / Q000166:cytology* / Q000201:enzymology; D011189:Potassium Chloride; D051381:Rats; D017207:Rats, Sprague-Dawley
D000682:Amyloid; D000911:Antibodies, Monoclonal; D003470:Culture Media; D011189:Potassium Chloride; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases