Plasmodium falciparum: naturally occurring rabbit immunoglobulins recognize human band 3 peptide motifs and malaria-infected red cells.
The reactivity to overlapping decapeptides based on sequences of human band 3 protein was determined for 24 rabbit serum samples. A high percentage of the sera recognized sequences of amino acid 650-670, as well as several other sequences representing putative exofacial regions of the band 3 protein. When sera were used to stain immunofluorescently human erythrocytes, some of which were infected with P. falciparum, those samples that predominantly reacted with amino acids 650-670 stained P. falciparum-infected red cells, whereas those that reacted with other regions of band 3 stained all erythrocytes. A positive correlation was found between anti-band 3 reactivity and the capacity of a serum to inhibit the cytoadherence of P. falciparum-infected erythrocytes to C32 amelanotic melanoma cells. Also, some rabbit sera immunoprecipitated a high-molecular-weight protein from extracts of surface iodinated P. falciparum-infected red cells.
Crandall
I
I
Department of Biology, University of California, Riverside 92521, USA.
Guthrie
N
N
Sherman
I W
IW
D000595:Amino Acid Sequence; D000818:Animals; D001457:Anion Exchange Protein 1, Erythrocyte / Q000276:immunology*; D002448:Cell Adhesion / Q000187:drug effects; D017124:Conserved Sequence; D003429:Cross Reactions; D000939:Epitopes; D004912:Erythrocytes / Q000187:drug effects; D005455:Fluorescent Antibody Technique; D006801:Humans; D007136:Immunoglobulins / Q000097:blood / Q000494:pharmacology; D016778:Malaria, Falciparum / Q000276:immunology*; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000276:immunology*; D011233:Precipitin Tests; D011817:Rabbits / Q000276:immunology*; D017386:Sequence Homology, Amino Acid
D001457:Anion Exchange Protein 1, Erythrocyte; D000939:Epitopes; D007136:Immunoglobulins; D010446:Peptide Fragments