Structural analysis of the predicted coiled-coil rod domain of the cytoplasmic bullous pemphigoid antigen (BPAG1). Empirical localization of the N-terminal globular domain-rod boundary.
The bullous pemphigoid antigen BPAG1 is required for keratin filament linkage to the hemidesmosome, an adhesion complex in epithelial basal cells. BPAG1 structural organization is similar to the intermediate filament-associated proteins desmoplakin I (DPI) and plectin. All three proteins have predicted dumbbell-like structure with central alpha-helical coiled-coil rod and regions of N- and C-terminal homology. To characterize the size of the N-terminal globular domain in BPAG1, two polypeptides spanning possible boundaries with the coiled-coil rod domain of BPAG1 were expressed in Escherichia coli. BP-1 (Mr = 111,000), containing amino acids 663-1581 of BPAG1 (Sawamura, D., Li, K., Chu, M.-L., and Uitto, J. (1991) J. Biol. Chem. 266, 17784-17790), and BP-1A, with a 186 amino acid N-terminal deletion, were purified. BP-1 and BP-1A behave as highly asymmetric dimers in aqueous solution according to velocity sedimentation and gel filtration. Both have globular heads with rod-like tails of roughly equal length, 55-60 nm, upon rotary shadowing. BP-1A content of alpha-helix, determined by circular dichroism, is approximately 90%, consistent with alpha-helical coiled-coil formation in the rod-like tails. The estimated rod length, 383 +/- 57 amino acids (0.15 nm/amino acid), implies that globular folding in the BPAG1 N-terminal extends to the end of N-terminal homology with DPI and plectin. These findings support the existence of a common domain structure in the N-terminal regions of the BPAG1/DPI/plectin family.
Tang
H Y
HY
Department of Medical Biochemistry and Genetics, Texas A & M College of Medicine, College Station, Texas 77843, USA.
Chaffotte
A F
AF
Thacher
S M
SM
D000595:Amino Acid Sequence; D001324:Autoantigens / Q000096:biosynthesis / Q000737:chemistry* / Q000648:ultrastructure; D002352:Carrier Proteins; D002942:Circular Dichroism; D003001:Cloning, Molecular; D003094:Collagen; D003598:Cytoskeletal Proteins / Q000737:chemistry; D051180:Desmoplakins; D000071821:Dystonin; D004848:Epithelium / Q000502:physiology; D006801:Humans; D007381:Intermediate Filament Proteins / Q000737:chemistry; D007633:Keratins / Q000502:physiology; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins; D024041:Non-Fibrillar Collagens; D010391:Pemphigoid, Bullous / Q000276:immunology*; D051190:Plectin; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000648:ultrastructure; D017386:Sequence Homology, Amino Acid; D000097805:Collagen Type XVII
D001324:Autoantigens; D002352:Carrier Proteins; D003094:Collagen; D003598:Cytoskeletal Proteins; D051180:Desmoplakins; D000071821:Dystonin; D007381:Intermediate Filament Proteins; D007633:Keratins; D009419:Nerve Tissue Proteins; D024041:Non-Fibrillar Collagens; D051190:Plectin; D011994:Recombinant Proteins; D000097805:Collagen Type XVII; C495294:DSP protein, human; C486268:DST protein, human; C495332:PLEC protein, human