title: Expression of alpha 6 and beta 4 integrins in serous ovarian carcinoma correlates with expression of the basement membrane protein laminin.
abstract: The surface of a normal ovary is covered by a monolayer of epithelial cells that rest on a basement membrane. The glycoprotein laminin is the major noncollagenous protein present in the basement membrane. The integrins alpha 1 beta 1, alpha 2 beta 1, alpha 3 beta 1, alpha 6 beta 1, and alpha 6 beta 4 serve as cell surface receptors for laminin. During the progression of serous ovarian carcinoma, tumor cells are frequently exfoliated from the surface of the ovary, thereby losing contact with the basement membrane. This study was designed to determine whether alterations in integrin expression may be associated with the malignant phenotype of the primary ovarian tumor and exfoliated ovarian carcinoma cells in the ascites fluid. By immunohistochemical staining, the entire surface of epithelial cells of normal ovaries stained positively for beta 1, alpha 2, and alpha 3 integrins, whereas only the basal surface of the epithelial cells, where they are in contact with laminin, stained positively for alpha 6 and beta 4. The entire surface of epithelial cells of solid tumors from patients with serous ovarian carcinoma stained positively for beta 1, alpha 2, and alpha 3 integrins. In most cases, no intact basement membrane surrounded the tumor nests, and staining for alpha 6 and beta 4 was irregular. When present, the basement membrane stained positively for laminin, and the basal surface of the epithelial cells stained positively for alpha 6 and beta 4. Ovarian carcinoma ascites cells exhibited a distinct phenotype, with a significant decrease in expression of the alpha 6 and beta 4 integrin subunits. As alpha 6 and beta 4 integrin subunits are present at the basal surface of many epithelial cells and serve as receptors for laminin, it is possible that ovarian carcinoma epithelial cells may be released from the basement membrane of the ovary due to their deficit of alpha 6 and beta 4 integrin subunits.
authors.affiliation: Department of Laboratory Medicine and Pathology, University of Minnesota Medical School, Minneapolis 55455-0315, USA.
authors.forename: A P
authors.initials: AP
authors.lastname: Skubitz
authors.forename: R C
authors.initials: RC
authors.lastname: Bast
authors.forename: E A
authors.initials: EA
authors.lastname: Wayner
authors.forename: P C
authors.initials: PC
authors.lastname: Letourneau
authors.forename: M S
authors.initials: MS
authors.lastname: Wilke
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000276:immunology; D015703:Antigens, CD / Q000032:analysis* / Q000276:immunology; D001485:Basement Membrane / Q000737:chemistry / Q000648:ultrastructure; D001706:Biopsy; D015820:Cadherins / Q000032:analysis / Q000276:immunology; D003094:Collagen / Q000032:analysis / Q000276:immunology; D018284:Cystadenocarcinoma, Serous / Q000737:chemistry* / Q000473:pathology / Q000648:ultrastructure; D004847:Epithelial Cells; D004848:Epithelium / Q000737:chemistry / Q000473:pathology; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D039503:Integrin alpha6; D039663:Integrin beta4; D015603:Keratinocytes / Q000737:chemistry; D007797:Laminin / Q000032:analysis*; D008875:Middle Aged; D010051:Ovarian Neoplasms / Q000737:chemistry* / Q000473:pathology / Q000648:ultrastructure; D010053:Ovary / Q000737:chemistry; D014407:Tumor Cells, Cultured
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D015820:Cadherins; D039503:Integrin alpha6; D039663:Integrin beta4; D007797:Laminin; D003094:Collagen