An on-line semi-automated solid-phase extraction procedure for high-performance liquid chromatographic determination of lonidamine in serum.
A semi-automated solid-phase extraction procedure on-line with gradient elution reversed-phase chromatography permits the determination of lonidamine and its metabolite in human serum. The average recovery from serum at the 2.5 micrograms ml-1 level was (92.8 +/- 3.4)%. The limit of quantitation for a 100 microliter sample size was 50 ng ml-1. The within-day (n = 5) and between-day (n = 5) relative standard deviations for lonidamine determination in serum samples spiked at the 2.5 micrograms ml-1 level were 2.7% and 4.5%, respectively.
Bottalico
C
C
Istituto Oncologico, Bari, Italy.
Micelli
G
G
Guerrieri
A
A
Palmisano
F
F
Lorusso
V
V
de Lena
M
M
D000970:Antineoplastic Agents / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006801:Humans; D007191:Indazoles / Q000097:blood*; D010051:Ovarian Neoplasms / Q000097:blood / Q000188:drug therapy
D000970:Antineoplastic Agents; D007191:Indazoles; C016371:lonidamine