A novel myb-related gene from Arabidopsis thaliana.
A novel myb-like gene (Atmyb5) has been isolated from a genomic library of Arabidopsis thaliana. The gene contains a single intron in the region coding for the Myb domains. The Myb domains are highly homologous to other animal and plant Myb proteins. Arabidopsis plants transgenic for a chimeric Atmyb5 promoter/GUS gene expressed the enzyme in a developmentally controlled and tissue specific manner. The GUS activity was detected in developing leaf trichomes, stipules, epidermal cells on the margins of young rosette and cauline leaves, and in immature seeds. Atmyb5 mRNA appears between fertilization and the 16 cell stage of embryo development and persists beyond the heart stage.
Li
S F
SF
School of Botany, La Trobe University, Bundoora, Melbourne, Victoria, Australia.
Santini
J M
JM
Nicolaou
O
O
Parish
R W
RW
D000595:Amino Acid Sequence; D000818:Animals; D017360:Arabidopsis / Q000166:cytology / Q000235:genetics* / Q000502:physiology; D029681:Arabidopsis Proteins; D001483:Base Sequence; D017124:Conserved Sequence; D017931:DNA Primers; D017343:Genes, Plant; D015698:Genomic Library; D005966:Glucuronidase / Q000096:biosynthesis; D008969:Molecular Sequence Data; D009857:Oncogenes; D018515:Plant Leaves; D010940:Plant Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D011518:Proto-Oncogene Proteins / Q000737:chemistry; D020598:Proto-Oncogene Proteins c-myb; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D012639:Seeds; D017386:Sequence Homology, Amino Acid; D015534:Trans-Activators / Q000737:chemistry
D029681:Arabidopsis Proteins; C099515:Atmyb5 protein, Arabidopsis; D017931:DNA Primers; D010940:Plant Proteins; D011518:Proto-Oncogene Proteins; D020598:Proto-Oncogene Proteins c-myb; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D015534:Trans-Activators; D005966:Glucuronidase