Catalytic activities of the human T-cell leukemia virus type II integrase.
Despite the widespread nature of HTLV-II in New World populations and intravenous drug users, the enzymatic activities of the pol genes have not been reported. To ascertain the activity of the HTLV-II(G12) integrase (IN), the coding region was isolated and the encoded protein was purified, using nickel-affinity chromatography, to greater than 90% homogeneity. HTLV-II(G12) IN proved active on HTLV-II(G12) and HIV-1 integration and disintegration substrates. Distinct differences in requirements for enzyme concentration for 3'-processing, strand-transfer, and disintegration reactions were observed. Catalysis of integration reactions occurred in the presence of either Mn2+ or Mg2+, although strand-transfer activity preferred Mn2+. In comparison, HTLV-II(G12) IN catalyzed disintegration reactions with almost 10-fold less protein, was not selective for Mn2+ or Mg2+, and tolerated higher NaCl concentrations than integration. HTLV-II(G12) IN was unable to catalyze the "splicing" reaction, which suggests that this may not be an activity ubiquitous to all retroviral integrases.
Balakrishnan
M
M
Graduate Program in Molecular Biology, New Mexico State University, Las Cruces, New Mexico 88003 USA.
Zastrow
D
D
Jonsson
C B
CB
D000818:Animals; D001483:Base Sequence; D002384:Catalysis; D004254:DNA Nucleotidyltransferases / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D017931:DNA Primers; D004926:Escherichia coli; D016325:HIV Long Terminal Repeat; D015497:HIV-1 / Q000235:genetics; D015367:Human T-lymphotropic virus 2 / Q000201:enzymology*; D006801:Humans; D019426:Integrases; D007477:Ions; D008670:Metals / Q000378:metabolism; D008969:Molecular Sequence Data; D009994:Osmolar Concentration; D011817:Rabbits; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D013696:Temperature; D016662:Virus Integration
D017931:DNA Primers; D007477:Ions; D008670:Metals; D011994:Recombinant Proteins; D004254:DNA Nucleotidyltransferases; D019426:Integrases