Interaction of NMDA and dopamine D2L receptors in human neuroblastoma SH-SY5Y cells.
To understand the mechanism of interaction of the dopamine D2L receptors with NMDA receptors, we have developed a model by transfecting human neuroblastoma SH-SY5Y cells with the human dopamine D2L receptor gene. In vitro blockade of NMDA receptors by the specific antagonists MK-801 and (+/-)-3-(2-carboxypiperazin-4-yl)-propyl-1-phosphonic acid (CPP) on human neuroblastoma SH-SY5Y cells expressing human dopamine D2L receptors resulted in a significant increase in the density of D2L receptors without a significant change in receptor affinity. Moreover, the dopamine receptor mRNA level increased by approximately 50% by the blockade of NMDA with MK-801. These results suggest a possible interaction of NMDA and dopamine D2L receptors in neuroblastoma SH-SY5Y cells. This system would serve as an excellent model to study the molecular mechanisms involved in the interaction of these two receptors.
Nair
V D
VD
Department of Psychiatry, McMaster University, Hamilton, Ontario, Canada.
Niznik
H B
HB
Mishra
R K
RK
D001483:Base Sequence; D016291:Dizocilpine Maleate / Q000494:pharmacology; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology; D006801:Humans; D008969:Molecular Sequence Data; D009447:Neuroblastoma; D010879:Piperazines / Q000494:pharmacology; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis; D017448:Receptors, Dopamine D2 / Q000235:genetics / Q000502:physiology*; D016194:Receptors, N-Methyl-D-Aspartate / Q000037:antagonists & inhibitors / Q000502:physiology*; D014162:Transfection; D014407:Tumor Cells, Cultured / Q000737:chemistry; D015854:Up-Regulation / Q000502:physiology
D018691:Excitatory Amino Acid Antagonists; D010879:Piperazines; D012333:RNA, Messenger; D017448:Receptors, Dopamine D2; D016194:Receptors, N-Methyl-D-Aspartate; D016291:Dizocilpine Maleate; C050592:3-(2-carboxypiperazin-4-yl)propyl-1-phosphonic acid