The reliability of the differential polymerase chain reaction compared to restriction fragment length polymorphism for the detection of gene loss in primary tumors.
The loss of genetic information from a number of specific regions of the genome has been documented in human tumors in vivo. To estimate the frequency with which IFN beta 1 gene loss occurs in human gliomas, both restriction fragment length polymorphism (RFLP) analysis and polymerase chain reaction (PCR) amplification, using the IFN-gamma gene as a reference, were used and the results obtained with the two methods were compared. The relative intensity of PCR bands (IFN beta 1/IFN gamma) in the gliomas with loss of heterozygosity for the IFN beta 1 was significantly different (P < 0.05) with regard to the gliomas with the IFN beta 1 gene. Consequently, these findings indicate that differential PCR is a reliable and non-radioactive method of demonstrating gene loss in tumors.
Sugawa
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Department of Neurosurgery, Kyoto Prefectural University of Medicine, Kyoto, Japan.
Ueda
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S
D000293:Adolescent; D000328:Adult; D000368:Aged; D000483:Alleles; D001483:Base Sequence; D002648:Child; D002675:Child, Preschool; D002872:Chromosome Deletion; D017931:DNA Primers; D004273:DNA, Neoplasm / Q000032:analysis; D005260:Female; D017353:Gene Deletion; D005910:Glioma / Q000235:genetics* / Q000473:pathology; D006801:Humans; D007371:Interferon-gamma / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D015203:Reproducibility of Results
D017931:DNA Primers; D004273:DNA, Neoplasm; D007371:Interferon-gamma