title: In vivo expression of inducible nitric oxide synthase in cerebellar neurons.
abstract: In the CNS, nitric oxide (NO) functions as both neuromodulator and neurotoxic agent. In vivo neuronal expression of NO synthase (NOS) has been attributed to constitutive NOS--both the neuronal and the endothelial types. The other class of NOS--the inducible NOS (iNOS)--is known to mediate toxic effects of NO in various tissues. In this study, we show for the first time that direct intracerebellar injection of endotoxin and cytokine (lipopolysaccharide and interferon-gamma) induced in vivo neuronal expression of the iNOS gene, as demonstrated by fluorescent in situ hybridization and immunohistochemical staining analyzed by confocal laser-scanning microscopy. This raises the possibility that neuronal iNOS might contribute significantly to the vulnerability of the brain to various insults.
authors.affiliation: Clinical Neuroscience Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland, USA.
authors.forename: D
authors.initials: D
authors.lastname: Minc-Golomb
authors.forename: G
authors.initials: G
authors.lastname: Yadid
authors.forename: I
authors.initials: I
authors.lastname: Tsarfaty
authors.forename: J H
authors.initials: JH
authors.lastname: Resau
authors.forename: J P
authors.initials: JP
authors.lastname: Schwartz
mesh_terms: D000818:Animals; D002531:Cerebellum / Q000166:cytology*; D015870:Gene Expression / Q000187:drug effects; D007150:Immunohistochemistry; D017404:In Situ Hybridization, Fluorescence; D007371:Interferon-gamma / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D008845:Microinjections; D018613:Microscopy, Confocal; D009474:Neurons / Q000201:enzymology*; D019001:Nitric Oxide Synthase / Q000032:analysis / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D014158:Transcription, Genetic / Q000187:drug effects
chemical_list: D008070:Lipopolysaccharides; D012333:RNA, Messenger; D007371:Interferon-gamma; D019001:Nitric Oxide Synthase