A 'mixed' self-assembled monolayer for an impedimetric immunosensor.
A synthetic peptide with the amino acid sequence 135-154 of the capsid protein VP1 of the foot-and-mouth-disease virus was modified with omega-hydroxyundecanethiol and applied together with non-derivatised omega-hydroxyundecanethiol for consecutive adsorption onto gold electrodes according to self-assembling procedures. The binding of a specific antibody to prepared recognition layers could be monitored by measurement of impedance or capacitance. In order to avoid non-specific effects, all measurements were performed in the presence of BSA. The complex between the antigenic peptide and the antibody was split by applying 6 M urea solution. The gold electrodes were mounted into an optimised flow-through system in order to perform capacitance-time measurements. The immobilised peptide can be recognised repeatedly by specific antibodies.
Rickert
J
J
Institute of Physical and Theoretical Chemistry, University of Tübingen, Germany.
Göpel
W
W
Beck
W
W
Jung
G
G
Heiduschka
P
P
D000595:Amino Acid Sequence; D000937:Antigen-Antibody Reactions; D015374:Biosensing Techniques; D002213:Capsid / Q000276:immunology; D036022:Capsid Proteins; D017097:Electric Impedance; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D010446:Peptide Fragments / Q000276:immunology
D036022:Capsid Proteins; D010446:Peptide Fragments