title: Human mineralocorticoid receptor interacts with actin under mineralocorticoid ligand modulation.
abstract: The human mineralocorticoid receptor of the steroid receptor family contains a modular structure with domain E which is considered to be a hormone binding domain. Recombinant protein approaches enabled us to clearly determine that this domain is also able to interact with F-actin (Kd about 2 microM) and G-actin. Moreover, it was revealed that this mineralocorticoid receptor domain/actin interaction was modulated by specific mineralocorticoid ligands.  Agonist (aldosterone) steroid binding almost totally (91%) abolished the interaction with F-actin, while antagonist (progesterone) binding allowed more than 30% of this binding. Steroid modulation of the interaction between domain E and actin indicated that this actin binding is specific and could be essential for cellular mineralocorticoid receptor activity.
authors.affiliation: Institut National de la Santé et de la Recherche Médicale, Unité 300, Faculté de Pharmacie, Montpellier, France.
authors.forename: S
authors.initials: S
authors.lastname: Jalaguier
authors.forename: D
authors.initials: D
authors.lastname: Mornet
authors.forename: D
authors.initials: D
authors.lastname: Mesnier
authors.forename: J J
authors.initials: JJ
authors.lastname: Léger
authors.forename: G
authors.initials: G
authors.lastname: Auzou
mesh_terms: D000199:Actins / Q000378:metabolism*; D000450:Aldosterone / Q000378:metabolism; D001665:Binding Sites; D006360:Heat-Shock Proteins / Q000378:metabolism; D006801:Humans; D008024:Ligands; D008901:Mineralocorticoids / Q000378:metabolism*; D010446:Peptide Fragments / Q000378:metabolism; D011374:Progesterone / Q000378:metabolism; D011485:Protein Binding; D018161:Receptors, Mineralocorticoid / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism
chemical_list: D000199:Actins; D006360:Heat-Shock Proteins; D008024:Ligands; D008901:Mineralocorticoids; D010446:Peptide Fragments; D018161:Receptors, Mineralocorticoid; D011993:Recombinant Fusion Proteins; D000450:Aldosterone; D011374:Progesterone