Stimulation by C-type natriuretic peptide of the differentiation of clonal osteoblastic MC3T3-E1 cells.
We examined the effects of C-type natriuretic peptide (CNP) and B-type natriuretic peptide receptor (NPR-B) system, which stimulates the intracellular production of cGMP, on osteoblastic differentiation using clonal murine calvarial MC3T3-E1 cells. CNP-like immunoreactivity was detected in the conditioned medium and in lysates of MC3T3-E1 cells. Exposure of cells to CNP caused an increase in the intracellular production of cGMP and the increase was dose-dependent, while ANP had no effect. These results imply that CNP regulates osteoblastic metabolism via NPR-B in an autocrine manner. Northern blot analysis revealed that treatment of MC3T3-E1 cells with CNP increased the steady-state levels of mRNAs for type-I collagen, cellular alkaline phosphatase (ALPase), and osteocalcin, which are well known as markers of osteoblastic differentiation. Our observations suggest the possibility that CNP functions as a local regulator of osteoblastic differentiation, acting via a cGMP-mediated pathway.
Inoue
A
A
Research Center for Experimental Biology, Tokyo Institute of Technology, Yokohama, Japan.
Hiruma
Y
Y
Hirose
S
S
Yamaguchi
A
A
Furuya
M
M
Tanaka
S
S
Hagiwara
H
H
D016475:3T3 Cells; D000818:Animals; D009320:Atrial Natriuretic Factor / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D006152:Cyclic GMP / Q000096:biosynthesis; D051379:Mice; D020098:Natriuretic Peptide, C-Type; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D011506:Proteins / Q000494:pharmacology*; D051381:Rats
D011506:Proteins; D020098:Natriuretic Peptide, C-Type; D009320:Atrial Natriuretic Factor; D006152:Cyclic GMP