title: Bedside measurement of factor VIII:C activity in individuals with hemophilia A.
abstract: Factor VIII replacement therapy for patients with hemophilia A is conventionally monitored using a plasma-based factor VIII:C assay (a modified activated partial thromboplastin time [APTT] test). The plasma factor VIII assay requires the preparation of plasma from citrated whole blood and measurement of the clotting times of mixtures of patient plasma, factor VIII-deficient substrate, and APTT reagent. Results are not routinely available in less than 1.5 hr, reducing the clinical value of the laboratory data regarding the ability to immediately adjust patient therapy. Results from the whole blood factor VIII assay, performed on a portable coagulation analyzer and using test tubes prefilled with the necessary APTT and factor VIII-deficient reagents, are available within 5-7 min. This immediate determination of the factor VIII:C level from citrated whole blood provides the opportunity to greatly reduce turnaround time and improve the efficacy of factor VIII replacement therapy. Based on clotting time, factor VIII:C activity is read from a standard curve. A clinical evaluation of this whole blood test was performed in two hemophilia centers. A high degree of correlation was seen (r=0.813, n=220) between the whole blood values obtained and conventional laboratory results. This level of correlation was superior to that obtained when comparing two different plasma-based systems(r=0.753, n=23). Factor VIII:C activity levels measured using the whole blood assay system were similar,irrespective of the test operator (laboratory technologist, nurse clinician, or patient). This study indicates that the whole blood factor VIII assay provides results comparable to those of conventional plasma-based assays, but in a more rapid and efficient manner. It provides an opportunity to reduce unnecessary patient consumption of replacement preparations, hence reducing the cost of hemophilia A maintenance and prophylaxis regimens, and to reduce overall patient exposure to human blood products.
authors.affiliation: Division of Hematology/Oncology, George Washington University Medical Center, Washington, DC, USA.
authors.forename: C M
authors.initials: CM
authors.lastname: Kessler
authors.forename: Z
authors.initials: Z
authors.lastname: Bernstein
authors.forename: S
authors.initials: S
authors.lastname: Ghesani
authors.forename: Z
authors.initials: Z
authors.lastname: Shamsipour
authors.forename: C
authors.initials: C
authors.lastname: Frances
authors.forename: M L
authors.initials: ML
authors.lastname: Zucker
authors.forename: F M
authors.initials: FM
authors.lastname: LaDuca
mesh_terms: D005169:Factor VIII / Q000032:analysis*; D006467:Hemophilia A / Q000097:blood*; D006801:Humans; D015588:Observer Variation; D010314:Partial Thromboplastin Time; D019095:Point-of-Care Systems / Q000706:statistics & numerical data; D012680:Sensitivity and Specificity
chemical_list: D005169:Factor VIII