SH3 domain-dependent interaction of the proto-oncogene product Vav with the focal contact protein zyxin.
Scr homology 3 (SH3) domain-mediated protein-protein interactions have been implicated in the localization of proteins to specific sites within the cell. We present evidence that the product of the vav proto-oncogene, p95vav, interacts specifically with the focal adhesion protein zyxin both in vitro and in yeast two hybrid system. Solution binding and two-hybrid system experiments demonstrate that association of Vav with the LIM domain protein zyxin is mediated by the C-terminal SH3 domain of the Vav and involves the proline-rich N-terminus of zyxin. The interaction appears to be selective, since no binding of the proline-rich N-terminus of zyxin with other SH3 domain-containing proteins such as GRB-2, phospholipase C gamma, GTPase-activating protein, or p85 was detected.
Hobert
O
O
Department of Molecular Biology, Max-Planck-Institut für Biochemie, Martinsried, Germany.
Schilling
J W
JW
Beckerle
M C
MC
Ullrich
A
A
Jallal
B
B
D000595:Amino Acid Sequence; D002448:Cell Adhesion; D018797:Cell Cycle Proteins; D002460:Cell Line; D003598:Cytoskeletal Proteins; D005982:Glutathione Transferase / Q000235:genetics; D006023:Glycoproteins; D006801:Humans; D008667:Metalloproteins / Q000378:metabolism*; D008969:Molecular Sequence Data; D011485:Protein Binding; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D051567:Proto-Oncogene Proteins c-vav; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D016335:Zinc Fingers; D060589:Zyxin; D018909:src Homology Domains
D018797:Cell Cycle Proteins; D003598:Cytoskeletal Proteins; D006023:Glycoproteins; C000715470:MAS1 protein, human; D008667:Metalloproteins; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins; D051567:Proto-Oncogene Proteins c-vav; D011993:Recombinant Fusion Proteins; C496618:VAV1 protein, human; C092842:ZYX protein, human; D060589:Zyxin; D005982:Glutathione Transferase