A calorimetric method for continuous recording of lymphocyte proliferation.
We have developed a calorimetric technique as an alternative non-radioactive method for measuring mitogen-induced lymphocyte proliferation. Power-time curves can be recorded from as few as 2X10(5) cells suspended in 4 ml medium. This density is, however, too low for the detection of proliferation during a 72 h incubation. Cell numbers exceeding 4X10(6) suspended in 4 ml medium cause pronounced crowding and are therefore too high for use during proliferation studies. We found the optimal initial cell concentration to be 2X10(6) cells suspended in 4 ml of medium. In a four-channel bioactivity monitor, up to four separate incubations can be run in parallel. Furthermore, this method offers the advantage of one-line monitoring of cell proliferation throughout the incubation.
Karlsson
H
H
Department of Medical Microbiology, Section of Clinical Immunology, University of Lund, Solvegatan 23, Sweden.
Nässberger
L
L
D002151:Calorimetry / Q000379:methods; D006801:Humans; D008213:Lymphocyte Activation; D015375:Receptors, Interleukin-2 / Q000032:analysis
D015375:Receptors, Interleukin-2