title: Thiocyanate-independent nitrosation in humans with carcinogenic parasite infection.
abstract: Infection with the liver fluke, Opisthorchis viverrini, is a causative agent of cholangiocarcinoma. One possible contributing factor in this carcinogenesis is the chronic, local generation of nitric oxide by inflammatory cells expressing inducible nitric oxide synthase and the production of N-nitroso compounds via the reaction between amines and nitrosating agents derived from nitric oxide. Our previous studies provided evidence that nitric oxide synthesis is elevated during human liver fluke infection. Here we present data on the same sample of men which definitively demonstrates increased nitrosation of proline and thioproline (thiazolidine-4-carboxylic acid) among infected men compared to uninfected control subjects on a low nitrate diet. This difference was specifically abolished by co-administration of ascorbic acid with proline and by elimination of parasites by praziquantel treatment. Multivariate statistical models demonstrate the importance of salivary thiocyanate levels to variation in the nitrosation of proline among uninfected individuals, but not among those with current fluke infection. This suggests that considerable generation of nitrosating agents (N203/N204) in infected people may be occurring via oxidation of arginine by nitric oxide synthase in inflamed tissue which is thiocyanate insensitive. Analyses revealed positive associations between N-nitrosoproline excretion and nitrate/nitrite levels in urine, plasma and saliva and with usual alcohol intake; with variation in these trends between groups. In conclusion, we have confirmed the relationship between O.viverrini infection and enhanced endogenous nitrosation, showing evidence of its extragastric site. New information is also provided on the determinants of N-nitrosamino acid excretion in men on a controlled low nitrate diet without smoking, conditions which reduce exogenous sources of nitrosating agents.
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Khon Kaen University, Thailand.
authors.forename: S
authors.initials: S
authors.lastname: Satarug
authors.forename: M R
authors.initials: MR
authors.lastname: Haswell-Elkins
authors.forename: M
authors.initials: M
authors.lastname: Tsuda
authors.forename: P
authors.initials: P
authors.lastname: Mairiang
authors.forename: P
authors.initials: P
authors.lastname: Sithithaworn
authors.forename: E
authors.initials: E
authors.lastname: Mairiang
authors.forename: H
authors.initials: H
authors.lastname: Esumi
authors.forename: S
authors.initials: S
authors.lastname: Sukprasert
authors.forename: P
authors.initials: P
authors.lastname: Yongvanit
authors.forename: D B
authors.initials: DB
authors.lastname: Elkins
mesh_terms: D000328:Adult; D000704:Analysis of Variance; D001650:Bile Duct Neoplasms / Q000209:etiology*; D001653:Bile Ducts, Intrahepatic; D018281:Cholangiocarcinoma / Q000209:etiology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009603:Nitroso Compounds / Q000378:metabolism*; D009889:Opisthorchiasis / Q000150:complications / Q000378:metabolism*; D011223:Praziquantel / Q000494:pharmacology; D013861:Thiocyanates / Q000378:metabolism
chemical_list: D009603:Nitroso Compounds; D013861:Thiocyanates; D011223:Praziquantel