Synthesis and properties of oligonucleotides containing the mutagenic base O4-benzylthymidine.
The preparation of synthetic oligodeoxynucleotides containing O4-benzylthymidine (Tbn) is described. The use of standard and t-butylphenoxyacetyl amino protecting groups is compared. The thermal stabilities of duplexes containing Tbn paired with adenine and guanine have been measured.
Fabrega
C
C
Department of Molecular Genetics, CID-CSIC, Barcelona, Spain.
Eritja
R
R
Sinha
N D
ND
Dosanjh
M K
MK
Singer
B
B
D001482:Base Composition; D001483:Base Sequence; D002942:Circular Dichroism; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009841:Oligonucleotides / Q000138:chemical synthesis*; D009943:Organophosphorus Compounds / Q000737:chemistry; D013936:Thymidine / Q000031:analogs & derivatives*
D009841:Oligonucleotides; D009943:Organophosphorus Compounds; D013936:Thymidine