Anti-TNF treatment reverts increased muscle ubiquitin gene expression in tumour-bearing rats.
Implantation of the ascitic tumour Yoshida AH-130 hepatoma (a cachectic tumour) resulted in important increases in muscle ubiquitin gene expression. Administration of daily injections of 25 mg/kg b.w. polyclonal goat anti-murine TNF IgG preparation to tumour-bearing rats abolished the increase in muscle ubiquitin gene expression observed in the control (non-anti-TNF-treated) tumour-bearing rats. It is concluded that TNF can have an important role in the activation of the ubiquitin-dependent proteolytic system during tumour growth.
Llovera
M
M
Departament de Bioquímica i Biologia Molecular, Universitat de Universitat de Barcelona, Spain.
Carbó
N
N
García-Martínez
C
C
Costelli
P
P
Tessitore
L
L
Baccino
F M
FM
Agell
N
N
Bagby
G J
GJ
López-Soriano
F J
FJ
Argilés
J M
JM
D000818:Animals; D000906:Antibodies / Q000276:immunology; D002100:Cachexia / Q000150:complications; D003546:Cysteine Endopeptidases / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic / Q000276:immunology*; D008114:Liver Neoplasms, Experimental / Q000150:complications / Q000235:genetics*; D008297:Male; D009097:Multienzyme Complexes / Q000235:genetics; D018482:Muscle, Skeletal / Q000378:metabolism*; D009368:Neoplasm Transplantation; D046988:Proteasome Endopeptidase Complex; D051381:Rats; D017208:Rats, Wistar; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors* / Q000276:immunology; D014452:Ubiquitins / Q000235:genetics*
D000906:Antibodies; D009097:Multienzyme Complexes; D014409:Tumor Necrosis Factor-alpha; D014452:Ubiquitins; D003546:Cysteine Endopeptidases; D046988:Proteasome Endopeptidase Complex