title: Gas chromatography/mass spectometry identification of long-term excreted metabolites of the anabolic steroid 4-chloro-1,2-dehydro-17alpha-methyltestosterone in humans.
abstract: The misuse of anabolic steroids by athletes has been banned by sports organizations and is controlled by the analysis of urine samples obtained from athletes using gas chromatography/mass spectrometry (GC/MS). To extend the retrospectivity of the analytical methods, research is focused on long-term excreted metabolites. Preliminary results concerning the long-term detection of metabolites of the anabolic androgenic steroid 4-chloro-1,2-dehydro-17alpha-methyltestosterone I are presented. A new metabolite 4-chloro-3alpha, 6 beta, 17beta-trihydroxy-17alpha-methyl-5beta-androst-l-en-16-one was isolated by high performance liquid chromatography (HPLC) from urine following a single oral administration of 40 mg of I and characterized. Metabolite II was excreted into urine with a maximum excretion rate at approximately 48 h after administration and could be detected by gas chromatography/high resolution mass spectrometry (GC/HRMS) for up to 14 days. Two further partly characterized metabolites III and IV were confirmed for more than 9 days. The same three metabolites, II-IV, in varying amounts were also detected in urine samples from athletes who administered I.
authors.affiliation: Institute of Biochemistry, German Sports University, Köln, Germany.
authors.forename: W
authors.initials: W
authors.lastname: Schanzer
authors.forename: S
authors.initials: S
authors.lastname: Horning
authors.forename: G
authors.initials: G
authors.lastname: Opfermann
authors.forename: M
authors.initials: M
authors.lastname: Donike
mesh_terms: D000328:Adult; D045930:Anabolic Agents / Q000737:chemistry* / Q000378:metabolism; D002849:Chromatography, Gas; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D008297:Male; D008777:Methyltestosterone / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism
chemical_list: D045930:Anabolic Agents; D008777:Methyltestosterone