The tRNATyr multigene family of Triticum aestivum: genome organization, sequence analyses and maturation of intron-containing pre-tRNAs in wheat germ extract.
Southern analysis of Triticum DNA has revealed that nuclear tRNATyr genes are dispersed at a minimum of 16 loci in the genome. We have isolated six independent tRNATyr genes from a Triticum aestivum library in addition to three known members of the Triticum tRNATyr family. Four of the sequenced tRNATyr genes code for Triticum tRNA Tyr and two code for tRNA2Tyr. Three genes encode tRNAsTyr which carry one or two nucleotide substitutions as compared to the conventional genes. The nine Triticum tRNATyr genes possess highly conserved intron sequences ranging in size from 12 to 14 nucleotides. A common secondary intron structure with the 5' and 3' splice site loops separated by five base pairs can be formed by all pre-tRNAs Tyr which are efficiently spliced in the homologous wheat germ extract.
Arends
S
S
Institut für Biochemie, Bayerishche Julius-Maximillans-Universität, Biozentrum, Würzburg, Germany.
Kraus
J
J
Beier
H
H
D001483:Base Sequence; D002457:Cell Extracts; D018744:DNA, Plant / Q000737:chemistry; D005091:Exons; D018745:Genome, Plant; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D005810:Multigene Family; D010936:Plant Extracts / Q000737:chemistry; D012322:RNA Precursors; D012323:RNA Processing, Post-Transcriptional; D012326:RNA Splicing; D012365:RNA, Transfer, Tyr / Q000737:chemistry* / Q000235:genetics*; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic; D014908:Triticum / Q000235:genetics*
D002457:Cell Extracts; D018744:DNA, Plant; D010936:Plant Extracts; D012322:RNA Precursors; D012365:RNA, Transfer, Tyr