MAT1, cdk7 and cyclin H form a kinase complex which is UV light-sensitive upon association with TFIIH.
MAT1, cyclin H and cdk7 are part of TFIIH, a class II transcription factor which possesses numerous subunits of which several have been shown to be involved in processes other than transcription. Two of them, XPD (ERCC2) and XPB (ERCC3), are helicases involved in nucleotide excision repair (NER), whereas cdk7, cyclin H and MAT1 are thought to participate in cell cycle regulation. MAT1, cyclin H and cdk7 exist as a ternary complex either free or associated with TFIIH from which the latter can be dissociated at high salt concentration. MAT1 is strongly associated with cdk7 and cyclin H. Although not strictly required for the formation and activity of the complex, it stimulates its kinase activity. The kinase activity of TFIIH, which is constant during the cell cycle, is reduced after UV light irradiation.
Adamczewski
J P
JP
Institut de Génétique et de Biologie Moléculaire et Cellulaire, UPR 6520 (CNRS), Unité 184 (INSERM), CU de Strasbourg, France.
Rossignol
M
M
Tassan
J P
JP
Nigg
E A
EA
Moncollin
V
V
Egly
J M
JM
D000595:Amino Acid Sequence; D000818:Animals; D002453:Cell Cycle; D002460:Cell Line; D056748:Cyclin H; D018844:Cyclin-Dependent Kinases; D016213:Cyclins / Q000737:chemistry* / Q000235:genetics / Q000528:radiation effects; D004249:DNA Damage; D004265:DNA Helicases / Q000737:chemistry / Q000235:genetics / Q000528:radiation effects; D004260:DNA Repair; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009097:Multienzyme Complexes / Q000737:chemistry / Q000235:genetics / Q000528:radiation effects; D009363:Neoplasm Proteins / Q000737:chemistry* / Q000235:genetics / Q000528:radiation effects; D011494:Protein Kinases / Q000737:chemistry / Q000235:genetics / Q000528:radiation effects; D017346:Protein Serine-Threonine Kinases / Q000737:chemistry* / Q000235:genetics / Q000528:radiation effects; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000528:radiation effects; D051758:Transcription Factor TFIIH; D014157:Transcription Factors / Q000737:chemistry* / Q000235:genetics / Q000528:radiation effects; D019473:Transcription Factors, TFII; D014466:Ultraviolet Rays; D000095043:Cyclin-Dependent Kinase-Activating Kinase
D056748:Cyclin H; D016213:Cyclins; D046911:Macromolecular Substances; D009097:Multienzyme Complexes; D009363:Neoplasm Proteins; D011994:Recombinant Proteins; D014157:Transcription Factors; D019473:Transcription Factors, TFII; D051758:Transcription Factor TFIIH; D011494:Protein Kinases; C060137:carboxy-terminal domain kinase; D017346:Protein Serine-Threonine Kinases; D018844:Cyclin-Dependent Kinases; D004265:DNA Helicases; D000095043:Cyclin-Dependent Kinase-Activating Kinase