title: Characterization of a cDNA and gene encoding the mouse theta class glutathione transferase mGSTT2 and its localization to chromosome 10B5-C1.
abstract: In this study, we have isolated and characterized a gene and cDNA encoding a mouse Theta class GST. The gene, mGSTT2, spans approximately 3.1 kb and is composed of five exons interrupted by four introns. The gene was localized to Chromosome 10B5-C1 by in situ hybridization. Southern blot analysis of mouse genomic DNA suggests that there is only one copy of mGSTT2 in the mouse genome. The cDNA derived from mGSTT2 was isolated from a mouse liver cDNA library and has an open reading frame of 732 bp encoding a peptide of 244 amino acids with a calculated molecular weight of 26,676 Da. The encoded protein shares amino acid sequence identities of 92, 77, 51, and 55% with rat subunit Yrs, human subunit GSTT2, rat subunit 5, and human subunit GSTT1, respectively.
authors.affiliation: Molecular Genetics Group,John Curtin School of Medical Research, Australian National University, Canberra, Australian Capital Territory, 2601, Australia.
authors.forename: A T
authors.initials: AT
authors.lastname: Whittington
authors.forename: G C
authors.initials: GC
authors.lastname: Webb
authors.forename: R T
authors.initials: RT
authors.lastname: Baker
authors.forename: P G
authors.initials: PG
authors.lastname: Board
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D018076:DNA, Complementary / Q000235:genetics; D005796:Genes; D005982:Glutathione Transferase / Q000235:genetics*; D006801:Humans; D017403:In Situ Hybridization; D007438:Introns; D051379:Mice; D008969:Molecular Sequence Data; D051381:Rats; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
chemical_list: D018076:DNA, Complementary; C484260:Gstt2 protein, mouse; C091152:Gstt2 protein, rat; D005982:Glutathione Transferase