Liquid chromatographic determination of mebendazole and its metabolites, aminomebendazole and hydroxymebendazole, in eel muscle tissue.
An analytical method is presented for liquid chromatographic (LC) determination of mebendazole (MBZ), hydroxymebendazole (MBZ-OH), and aminomebendazole (MBZ-NH2) in eel muscle tissue. Muscle tissue is extracted with ethyl acetate at pH 7.5. After addition of n-hexane, the extract is cleaned up and concentrated on an aminopropyl solid-phase extraction column. The test solutions are analyzed isocratically on a ChromSpher B LC column with acetonitrile-phosphate buffer, pH 6.2, as mobile phase. Limits of detection and quantitation were 0.7 and 1.1 micrograms/kg, respectively, for MBZ-OH; 1.4 and 2.3 micrograms/kg, respectively, for MBZ; and 1.5 and 2.1 micrograms/kg, respectively, for MBZ-NH2- Interand intraday coefficients of variation were 3.5 and 3.4%, respectively, for MBZ-OH; 2.5 and 3.1%, respectively, for MBZ; and 5.8 and 4.8%, respectively, for MBZ-NH2. Mean recoveries were 90% for MBZ, 74% for MBZ-NH2, and 92% for MBZ-OH. A linear range of applicability of at least 10-1000 micrograms/kg was found for each analyte. Incurred MBZ-NH2 (181.3 micrograms/kg) was identified in eel muscle tissue apart from MBZ (23.7 micrograms/kg) after 48 h exposure in a treatment bath containing MBZ at 1 mg/L.
Hajee
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University of Utrecht, Faculty of Veterinary Medicine, Department of the Science of Food of Animal Origin, The Netherlands.
Haagsma
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D000818:Animals; D000969:Antinematodal Agents / Q000032:analysis*; D002853:Chromatography, Liquid; D004524:Eels / Q000378:metabolism*; D008463:Mebendazole / Q000032:analysis* / Q000378:metabolism; D009132:Muscles / Q000737:chemistry*
D000969:Antinematodal Agents; D008463:Mebendazole