Complete cDNA sequence of the preproform of human pregnancy-associated plasma protein-A. Evidence for expression in the brain and induction by cAMP.
A cDNA that encodes the prepropeptide of pregnancy-associated plasma protein-A (preproPAPP-A), a putative metalloproteinase, has been cloned and sequenced. PAPP-A is synthesized in the placenta as a 1627-residue precursor preproprotein with a putative 22-residue signal peptide and a highly basic propeptide of 58 residues. The prepro-PAPP-A-encoding transcript contains a region with an extremely high G+C content and has an unusually long 5' untranslated region with several upstream short ORF. No alternatively spliced products could be identified by means of Northern blotting experiments or with rapid amplification of 5' cDNA ends experiments. A stretch within the 5' untranslated region shows sequence identities to a partial cDNA isolated from brain and a to cAMP-inducible sequence from a choriocarcinoma cell line.
Haaning
J
J
Department of Molecular and Structural Biology, University of Aarhus, Denmark.
Oxvig
C
C
Overgaard
M T
MT
Ebbesen
P
P
Kristensen
T
T
Sottrup-Jensen
L
L
D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000494:pharmacology*; D000595:Amino Acid Sequence; D001483:Base Sequence; D001921:Brain / Q000378:metabolism*; D002460:Cell Line; D003001:Cloning, Molecular; D018076:DNA, Complementary; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011266:Pregnancy-Associated Plasma Protein-A / Q000235:genetics*; D011498:Protein Precursors / Q000096:biosynthesis / Q000235:genetics*; D014327:Trophoblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism
D018076:DNA, Complementary; D011498:Protein Precursors; D015124:8-Bromo Cyclic Adenosine Monophosphate; D011266:Pregnancy-Associated Plasma Protein-A