Mapping of HPV-11 E1 binding site and determination of other important cis elements for replication of the origin.
The viral proteins E1 and E2 are essential for the replication of the HPV-11 origin. We have now mapped the E1 binding site (E1BS) and show by mutation analysis that the E1BS and an adjoining poly(A)-rich region are necessary for efficient replication of the origin. We have also shown, using suboptimal levels of E1 protein, that enhancement of E1 binding by E2 partially protects the E1BS. Finally, we show that E1 can enhance the binding of E2 even in the absence of the E1BS.
Sun
Y N
YN
Department of Microbiology and Immunology, University of Rochester, New York 14642, USA.
Lu
J Z
JZ
McCance
D J
DJ
D001483:Base Sequence; D001665:Binding Sites; D018983:DNA Footprinting; D004275:DNA, Ribosomal / Q000235:genetics; D004279:DNA, Viral / Q000096:biosynthesis* / Q000235:genetics; D004268:DNA-Binding Proteins / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation; D027383:Papillomaviridae / Q000235:genetics* / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D012045:Regulatory Sequences, Nucleic Acid; D018741:Replication Origin / Q000235:genetics*; D014764:Viral Proteins / Q000378:metabolism*
D004275:DNA, Ribosomal; D004279:DNA, Viral; D004268:DNA-Binding Proteins; C084304:E1 protein, Human papillomavirus type 11; C051550:E2 protein, Bovine papillomavirus; D011993:Recombinant Fusion Proteins; D014764:Viral Proteins