title: Characterization of a cDNA encoding cysteine proteinase inhibitor from Chinese cabbage (Brassica campestris L. ssp. pekinensis) flower buds.
abstract: A cDNA encoding a new phytocystatin isotype named BCPI-1 was isolated from a cDNA library of Chinese cabbage flower buds. The BCPI-1 clone encodes 199 amino acids resulting in a protein much larger than other known phytocystatins. BCPI-1 has an unusually long C-terminus. A BCPI-1 fusion protein expressed in Escherichia coli strongly inhibits the enzymatic activity of papain, a cysteine proteinase. Genomic Southern blot analysis revealed that the BCPI gene is a member of a small multi-gene family in Chinese cabbage. Northern blot analysis showed that it is differentially expressed in the flower bud, leaf and root.
authors.affiliation: Plant Molecular Biology and Biotechnology Research Center, Chinju, Korea.
authors.forename: C O
authors.initials: CO
authors.lastname: Lim
authors.forename: S I
authors.initials: SI
authors.lastname: Lee
authors.forename: W S
authors.initials: WS
authors.lastname: Chung
authors.forename: S H
authors.initials: SH
authors.lastname: Park
authors.forename: I
authors.initials: I
authors.lastname: Hwang
authors.forename: M J
authors.initials: MJ
authors.lastname: Cho
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D001937:Brassica / Q000235:genetics*; D015853:Cysteine Proteinase Inhibitors / Q000096:biosynthesis / Q000235:genetics* / Q000494:pharmacology; D018076:DNA, Complementary / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D018628:Gene Dosage; D017343:Genes, Plant; D008969:Molecular Sequence Data; D005810:Multigene Family; D010206:Papain / Q000037:antagonists & inhibitors; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000494:pharmacology; D018520:Plant Shoots; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000494:pharmacology; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: C099228:BCPI-1 protein, Brassica rapa; D015853:Cysteine Proteinase Inhibitors; D018076:DNA, Complementary; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; D010206:Papain