Liposome-mediated delivery of photosensitizers: localization of zinc (II)-phthalocyanine within implanted tumors after intravenous administration.
CGP55847, liposomal zinc(II)-phthalocyanine (Zn-Pc), was administered by the intravenous route to Swiss mice bearing intramuscularly implanted Ehrlich carcinomas or to C57/BL6 mice bearing subcutaneously implanted B16 melanomas. Tumors were removed 3 h or 24 h after dosing the intratumoral distribution determined by fluorescence microscopy. Localization of the photosensitizer occurred more rapidly in the Ehrlich carcinoma than in the B16 melanoma; this difference in photosensitizer uptake may be related to a higher degree of vascularization of the carcinoma. The photosensitizer was found in association with blood vessels at 3 h but not 24 h after dosing and appeared to have a greater affinity for areas of tissue necrosis within the tumor compared to viable tumor tissue. Little or no Zn-Pc was detected in the muscle tissue invaded by the Ehrlich carcinoma and was associated with the membranes and the cytosol, but not the nucleus, of cells in both tumors.
Love
W G
WG
Ciba Pharmaceuticals, Horsham, West Sussex, UK.
Duk
S
S
Biolo
R
R
Jori
G
G
Taylor
P W
PW
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000473:pathology*; D004337:Drug Carriers; D005189:False Positive Reactions; D007211:Indoles / Q000008:administration & dosage* / Q000032:analysis / Q000493:pharmacokinetics; D007275:Injections, Intravenous; D054833:Isoindoles; D008081:Liposomes; D008546:Melanoma, Experimental / Q000098:blood supply / Q000473:pathology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008856:Microscopy, Fluorescence; D018482:Muscle, Skeletal / Q000473:pathology; D009336:Necrosis; D009942:Organometallic Compounds / Q000008:administration & dosage* / Q000032:analysis / Q000493:pharmacokinetics; D017319:Photosensitizing Agents / Q000008:administration & dosage* / Q000032:analysis / Q000493:pharmacokinetics; D014018:Tissue Distribution; D015032:Zinc; D017967:Zinc Compounds
D004337:Drug Carriers; D007211:Indoles; D054833:Isoindoles; D008081:Liposomes; D009942:Organometallic Compounds; D017319:Photosensitizing Agents; D017967:Zinc Compounds; C052159:Zn(II)-phthalocyanine; D015032:Zinc