Effects of cell cycle dependent histone H1 phosphorylation on chromatin structure and chromatin replication.
We have reconstituted salt-treated SV40 minichromosomes with differentially phosphorylated forms of histone H1 extracted from either G0-, S- or M-phase cells. Sedimentation studies revealed a clear difference between minichromosomes reconstituted with S-phase histone H1 compared with histone H1 from G0- or M-phase cells, indicating that the phosphorylation state of histone H1 has a direct effect on chromatin structure. Using reconstituted minichromosomes as substrate in the SV40 in vitro replication system, we measured a higher replication efficiency for SV40 minichromosomes reconstituted with S-phase histone H1 compared with G0- or M-phase histone H1. These data indicate that the chromatin structure induced by the phosphorylation of histone H1 influences the replication efficiency of SV40 minichromosomes in vitro.
Halmer
L
L
Division of Biology, Universität Konstanz, Germany.
Gruss
C
C
D000818:Animals; D002453:Cell Cycle; D002460:Cell Line; D002522:Chlorocebus aethiops; D002843:Chromatin / Q000378:metabolism / Q000648:ultrastructure*; D004261:DNA Replication; D004279:DNA, Viral / Q000378:metabolism / Q000648:ultrastructure*; D006657:Histones / Q000302:isolation & purification / Q000378:metabolism*; D008938:Mitosis; D010766:Phosphorylation; D016192:Resting Phase, Cell Cycle; D016196:S Phase; D013539:Simian virus 40 / Q000235:genetics* / Q000502:physiology
D002843:Chromatin; D004279:DNA, Viral; D006657:Histones