Studies of the activities of lysosomal enzymes in serum and buccal pouch tissue of hamsters during 7,12-dimethylbenz[a]anthracene-induced carcinogenesis.
Alterations in the activities of certain lysosomal enzymes such as beta-D-galactosidase, beta-D-glucosidase, beta-D-glucuronidase, alpha-L-fucosidase, N-acetyl-beta-D-glucosaminidase, cathepsins B and D were studied in serum and tissue homogenates of buccal mucosa of hamsters treated with 0.5%, 7,12-dimethylbenz[a]anthracene (DMBA) in liquid paraffin. Among the enzymes studied, the activities of beta-D-galactosidase and N-acetyl-beta-D-glucosaminidase showed significant elevation both in serum and tissue homogenates fro papilloma onwards and the elevations were progressive with the development of carcinomas. The elevations in the activities of alpha-D-fucosidase and cathepsin D were found to be significant from papillomatous tissue onwards whereas in serum they showed higher activities only in carcinoma stages. The activities of beta-D-glucosidase, beta-D-glucuronidase and cathepsin B in both serum and in tissue homogenate were elevated markedly only in carcinoma stages. It is suggested that beta-D-galactosidase and N-acetyl-beta-D-glucosaminidase may be used as diagnostic markers for premalignant and malignant lesions of oral mucosa.
Balasubramanian
S
S
Department of Biochemistry, University of Madras, Guindy Campus, India.
Nagarajan
B
B
Govindasamy
S
S
D015127:9,10-Dimethyl-1,2-benzanthracene; D000118:Acetylglucosaminidase / Q000378:metabolism; D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000139:chemically induced / Q000201:enzymology*; D002401:Cathepsin B / Q000378:metabolism; D002402:Cathepsin D / Q000378:metabolism; D002610:Cheek / Q000473:pathology; D006224:Cricetinae; D005966:Glucuronidase / Q000378:metabolism; D006965:Hyperplasia / Q000139:chemically induced / Q000201:enzymology; D008297:Male; D008647:Mesocricetus; D009062:Mouth Neoplasms / Q000139:chemically induced / Q000201:enzymology*; D010212:Papilloma / Q000139:chemically induced / Q000201:enzymology*; D011230:Precancerous Conditions / Q000139:chemically induced / Q000201:enzymology*; D005644:alpha-L-Fucosidase / Q000378:metabolism; D001616:beta-Galactosidase / Q000378:metabolism
D015127:9,10-Dimethyl-1,2-benzanthracene; D001616:beta-Galactosidase; D005966:Glucuronidase; D005644:alpha-L-Fucosidase; D000118:Acetylglucosaminidase; D002401:Cathepsin B; D002402:Cathepsin D