Flow cytometric analysis of T and Tn epitopes on chronic lymphocytic leukemia cells.
Immunophenotyping of peripheral blood lymphocytes from six patients with B-cell chronic lymphocytic leukemia (B-CLL) and five normal volunteers was done and their T and Tn epitopes analyzed using specific monoclonal antibodies and flow cytometry. Lymphocytes from all patients showed strong Tn expression as compared to normal control lymphocytes. By contrast, T antigen was not expressed, The TN expression may be a useful diagnostic and prognostic marker for B-CLL.
Aller
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Department of Microbiology and Immunology, Finch University Health Science, Chicago Medical School, North Chicago, Illinois 60064, USA.
Kucuk
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Springer
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Gilman-Sachs
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D000328:Adult; D000368:Aged; D015703:Antigens, CD / Q000032:analysis; D000951:Antigens, Neoplasm / Q000032:analysis*; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000032:analysis*; D001402:B-Lymphocytes / Q000276:immunology*; D014408:Biomarkers, Tumor / Q000032:analysis*; D000939:Epitopes / Q000032:analysis*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D016130:Immunophenotyping / Q000379:methods*; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D014411:Neoplastic Stem Cells / Q000276:immunology*
D015703:Antigens, CD; D000951:Antigens, Neoplasm; D015295:Antigens, Tumor-Associated, Carbohydrate; D014408:Biomarkers, Tumor; D000939:Epitopes; C040286:Tn antigen; C016467:Thomsen-Friedenreich antigen