Cloning and characterization of cDNAs for novel proteins with glutamic acid-proline dipeptide tandem repeats.
cDNAs with identical 3' sequences containing a hexanucleotide repeat -(GAGCCG)9- were isolated from rat pheochromocytoma and brain cDNA libraries. The cDNA with the longest open reading frame codes for a protein of 24.6 kDa containing a 16-fold -(Glu-Pro)- dipeptide repeat within a glutamate and proline rich region at its deduced C-terminus. cDNAs with the identical 3' sequence and a divergent 5' sequence were isolated from a rat skeletal muscle cDNA library. The latter are predicted to code for a protein of 15.5 kDa with a C-terminal repetitive domain identical to that in the pheochromocytoma and brain cDNAs. The cDNAs recognize a 1.8 kb mRNA species present in a variety of tissues, being particularly abundant in cardiac and skeletal muscle.
Geertman
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Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla 10595, USA.
McMahon
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A
Sabban
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D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000196:embryology; D002478:Cells, Cultured; D003001:Cloning, Molecular; D018076:DNA, Complementary; D018698:Glutamic Acid / Q000235:genetics*; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000378:metabolism; D016366:Open Reading Frames; D010673:Pheochromocytoma / Q000235:genetics; D011392:Proline / Q000235:genetics*; D011506:Proteins / Q000235:genetics*; D051381:Rats; D012091:Repetitive Sequences, Nucleic Acid; D014407:Tumor Cells, Cultured
D018076:DNA, Complementary; D011506:Proteins; D018698:Glutamic Acid; D011392:Proline