Association of the expression of an SR-cyclophilin with myeloid cell differentiation.
The role of a 150-kD SR-cyclophilin (NK-TR1) in monocyte differentiation was investigated. Using an antipeptide monoclonal antibody, we have detected NK-TR1 in human peripheral blood monocytes and HL-60 cells. Unstimulated monocytes showed a low intracellular level of NK-TR1 protein that increased over 3 days of lipopolysaccharide + interferon-gamma treatment, consistent with the kinetics of monocyte differentiation. Normal HL-60 cells also had a low level of NK-TR1 protein, and exposure to 1.25% dimethyl sulfoxide (DMSO) resulted in a marked transient increase in expression that returned to basal levels before the development of granulocyte differentiation-associated biochemical changes. Phorbol myristate acetate, a promoter of monocytic differentiation in HL-60 cells, also caused a significant increase in NK-TR1 over basal levels. Transfection of a vector expressing NK-TR1 antisense RNA into HL-60 cells suppressed DMSO-mediated growth arrest. In addition, the development of a more mature phenotype, as measured by expression of CD16, and the ability to reduce nitroblue tetrazoleum dye was inhibited in transfectants when compared with controls. These results are consistent with the hypothesis that the NK-TR1 gene product is required for the progression towards a mature differentiated phenotype.
Giardina
S L
SL
Biological Carcinogenesis and Development Program, SAIC Frederick, MD 21702-1201, USA.
Coffman
J D
JD
Young
H A
HA
Potter
S J
SJ
Frey
J L
JL
Ortaldo
J R
JR
Anderson
S K
SK
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001798:Blood Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D002454:Cell Differentiation / Q000235:genetics*; D002522:Chlorocebus aethiops; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D005260:Female; D005786:Gene Expression Regulation; D018922:HL-60 Cells; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology; D007694:Killer Cells, Natural; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000473:pathology; D015459:Leukemia-Lymphoma, Adult T-Cell / Q000473:pathology; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D009000:Monocytes / Q000166:cytology*; D009580:Nitroblue Tetrazolium; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D010084:Oxidation-Reduction; D051381:Rats; D017452:Receptors, IgG / Q000096:biosynthesis / Q000235:genetics; D055607:Receptors, Natural Killer Cell; D011994:Recombinant Proteins / Q000378:metabolism; D014162:Transfection; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D001798:Blood Proteins; D008070:Lipopolysaccharides; D016376:Oligonucleotides, Antisense; D017452:Receptors, IgG; D055607:Receptors, Natural Killer Cell; D011994:Recombinant Proteins; D009580:Nitroblue Tetrazolium; D007371:Interferon-gamma; D004121:Dimethyl Sulfoxide