From DNA sequence to biological function.
Genome sequencing is leading to the discovery of new genes at a rate 50-100 times greater than that achieved by classical genetics, but the biological function of almost half of these genes is completely unknown. In order fully to exploit genome sequence data, a systematic approach to the discovery of gene function is required. Possible strategies are discussed here in the context of functional analysis in the yeast Saccharomyces cerevisiae, a model eukaryote whose genome sequence will soon be completed.
Oliver
S G
SG
Department of Biochemistry and Applied Molecular Biology, UMIST, Manchester, UK.
D001483:Base Sequence; D004271:DNA, Fungal; D005800:Genes, Fungal / Q000502:physiology*; D016678:Genome; D006801:Humans; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010641:Phenotype; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
D004271:DNA, Fungal