Effects of sodium sulfite on recovery and composition of detergent fiber and lignin.
Use of sodium sulfite to reduce nitrogenous contamination in fiber analysis was evaluated. The effects of sodium sulfite on analytical accuracy and precision were examined for amylase-treated neutral detergent fiber (aNDF), sequentially determined acid detergent fiber (sADF), and acid detergent lignin (sADL) in animal feeds. In one experiment, 0.5 g sodium sulfite was added per sample during neutral detergent (ND) extraction. The treatment consistently reduced aNDF, sADF, and sADL values of 180 alfalfa samples and improved precision (decreased within-sample variance of replicated analyses). The greatest effect was on precision of sADL analysis, with within-sample variance reduced by more than 50%. In a second experiment, 24 animal feeds were analyzed for a aNDF, sADF, and sADL with and without addition of 0.5 g sodium sulfite per sample during ND extraction. Nitrogen contents of the recovered fiber fractions were determined. Sodium sulfite reduced fiber and lignin values and decreased nitrogen concentration in residues. Within-sample variance was lower in all analyses. In a third experiment, 23 animal feeds were analyzed for aNDF with sodium sulfite at 0, 0.25, 0.5, and 1.0 g per sample. Average aNDF of feeds was reduced by each additional increment of sodium sulfite; however, 1.0 g sodium sulfite resulted in only a slight reduction in aNDF compared with 0.5 g. Therefore, 0.5 g sodium sulfite per sample should be added to samples prior to aNDF analysis.
Hintz
R W
RW
U.S. Department of Agriculture Research Service, U.S. Dairy Forage Research Center, Madison, WI 53706, USA.
Mertens
D R
DR
Albrecht
K A
KA
D000681:Amylases / Q000494:pharmacology; D000821:Animal Feed / Q000032:analysis*; D003902:Detergents / Q000032:analysis*; D004043:Dietary Fiber / Q000032:analysis*; D005504:Food Analysis / Q000706:statistics & numerical data*; D006863:Hydrogen-Ion Concentration; D008031:Lignin / Q000032:analysis*; D000455:Medicago sativa / Q000737:chemistry*; D009584:Nitrogen / Q000032:analysis; D012680:Sensitivity and Specificity; D013447:Sulfites / Q000494:pharmacology*
D003902:Detergents; D004043:Dietary Fiber; D013447:Sulfites; D008031:Lignin; D000681:Amylases; D009584:Nitrogen; C025026:sodium sulfite