title: Nitric oxide and superoxide anion production decrease with age in resident and activated rat peritoneal macrophages.
abstract: Superoxide anion and nitric oxide production have been studied in resident and activated peritoneal macrophages of 3-, 12-, and 24-month-old rats. Some key enzymes involved in the metabolism of glucose were also studied in relation to aging. Production of O2 and NO was reduced in all cases in middle-aged (12 months) and old (24 months) animals. Malic enzyme and citrate synthase activity shows a progressive reduction with age. Hexoquinase, pyruvate quinase, and lactate dehydrogenase activities decrease sharply from 3 to 12 months with no significant change between 12 and 24 months. Taken as a whole, the results of enzyme activity suggest that aging may reduce the capacity for glucose utilization in macrophages.
authors.affiliation: Departamento de Bioquimica Medica y Biologia Molecular, Facultad de Medicina, Seville, Spain.
authors.forename: E
authors.initials: E
authors.lastname: Alvarez
authors.forename: A
authors.initials: A
authors.lastname: Machado
authors.forename: F
authors.initials: F
authors.lastname: Sobrino
authors.forename: C
authors.initials: C
authors.lastname: Santa Maria
mesh_terms: D000375:Aging / Q000378:metabolism*; D000818:Animals; D002478:Cells, Cultured; D008262:Macrophage Activation; D017737:Macrophages, Peritoneal / Q000378:metabolism*; D009569:Nitric Oxide / Q000096:biosynthesis*; D051381:Rats; D013481:Superoxides / Q000378:metabolism*
chemical_list: D013481:Superoxides; D009569:Nitric Oxide