title: Improved diagnosis of cowpea aphid-borne mosaic virus in Africa: significance for cowpea seed-indexing, breeding programs and potyvirus taxonomy.
abstract: Large-scale surveys in Africa for blackeye cowpea mosaic (B1CMV) and cowpea aphid-borne mosaic (CABMV) showed that several CABMV isolates from Southern Africa were either not or poorly recognized by monoclonal antibodies prepared to isolates collected in West Africa. Selection of three new monoclonal antibodies prepared against the Maputo (Mozambique) isolate of CABMV, and their incorporation into a revised panel of monoclonal antibodies, resulted in the assignment of four of these new CABMV isolates to existing serotypes (II, IV, and V) and three others to a new serotype (VI). The South African isolate of passiflora mosaic virus was shown to be related to CABMV isolates in serotype IV. It is proposed that CABMV isolates be assembled into a distinct species in the legume-infecting, aphid-transmissible potyviruses.
authors.affiliation: Pacific Agriculture Research Centre, Agriculture and Agri-Food Canada.
authors.forename: C
authors.initials: C
authors.lastname: Huguenot
authors.forename: M T
authors.initials: MT
authors.lastname: Furneaux
authors.forename: J A
authors.initials: JA
authors.lastname: Clare
authors.forename: R I
authors.initials: RI
authors.lastname: Hamilton
mesh_terms: D000349:Africa; D000353:Africa, Southern; D000818:Animals; D001042:Aphids / Q000821:virology*; D007887:Fabaceae / Q000821:virology*; D005843:Geography; D009029:Mosaic Viruses / Q000145:classification* / Q000302:isolation & purification / Q000502:physiology; D010935:Plant Diseases / Q000821:virology; D010946:Plants, Medicinal; D017800:Potyvirus / Q000145:classification* / Q000302:isolation & purification / Q000502:physiology