title: Expression of IFN regulatory factor family proteins in lymphocytes. Induction of Stat-1 and IFN consensus sequence binding protein expression by T cell activation.
abstract: Interferon consensus sequence binding protein (ICSBP) is a transcription factor of the IFN regulatory factor (IRF) family. Evidence indicates that this family has a function in the immune system. Unlike other members of the family, ICSBP is expressed exclusively in the immune system. In this work, immunoblot analysis was performed to study expression of ICSBP and other members of the family in various murine lymphocytes. The results show that all IRF family members are expressed constitutively in B cells throughout development, and in resting and activated cells. In contrast, ICSBP expression was undetectable in thymocytes and resting T cells, while all other IRF proteins tested (IRF-1, IRF-2, and ISGF3-gamma) were detected in these cells. Induction of ICSBP (and weakly IRF-1, but not other members) was observed upon activation of T cells following anti-CD3 Ab binding or Con A stimulation. Once T cells were activated, ICSBP was expressed stably in both Th1 and Th2 cells. We show that Stat-1, which binds to the IFN-gamma-responsive element of the ICSBP promoter, was induced following anti-CD3 Ab and Con A stimulation. Stat-1 induction was found in T cells of IFN-gamma+/+, but not of IFN-gamma-/- mice, indicating that T cell activation stimulates the Stat pathway of transcription that is mediated through IFN-gamma. IFN-gamma-activated Stat-1 partly accounted for ICSBP induction in activated T cells, as levels of induction were lower in IFN-gamma-/- than in IFN+/+ T cells. Taken together, these results show that activation of ICSBP is coupled with T cell activation that is partly due to IFN-gamma-induced Stat-1.
authors.affiliation: Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, Bethesda, MD 20892, USA.
authors.forename: N
authors.initials: N
authors.lastname: Nelson
authors.forename: Y
authors.initials: Y
authors.lastname: Kanno
authors.forename: C
authors.initials: C
authors.lastname: Hong
authors.forename: C
authors.initials: C
authors.lastname: Contursi
authors.forename: T
authors.initials: T
authors.lastname: Fujita
authors.forename: B J
authors.initials: BJ
authors.lastname: Fowlkes
authors.forename: E
authors.initials: E
authors.lastname: O'Connell
authors.forename: J
authors.initials: J
authors.lastname: Hu-Li
authors.forename: W E
authors.initials: WE
authors.lastname: Paul
authors.forename: D
authors.initials: D
authors.lastname: Jankovic
authors.forename: A F
authors.initials: AF
authors.lastname: Sher
authors.forename: J E
authors.initials: JE
authors.lastname: Coligan
authors.forename: A
authors.initials: A
authors.lastname: Thornton
authors.forename: E
authors.initials: E
authors.lastname: Appella
authors.forename: Y
authors.initials: Y
authors.lastname: Yang
authors.forename: K
authors.initials: K
authors.lastname: Ozato
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D001483:Base Sequence; D017252:CD3 Complex / Q000276:immunology; D002352:Carrier Proteins / Q000096:biosynthesis*; D002454:Cell Differentiation / Q000276:immunology; D002460:Cell Line; D004268:DNA-Binding Proteins / Q000096:biosynthesis*; D006825:Hybridomas / Q000378:metabolism; D050856:Interferon Regulatory Factor-1; D050835:Interferon Regulatory Factors; D007371:Interferon-gamma / Q000235:genetics / Q000502:physiology*; D007399:Interphase / Q000276:immunology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008213:Lymphocyte Activation; D051379:Mice; D008969:Molecular Sequence Data; D010750:Phosphoproteins / Q000096:biosynthesis; D011948:Receptors, Antigen, T-Cell / Q000502:physiology; D012097:Repressor Proteins; D050794:STAT1 Transcription Factor; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism*; D018417:Th1 Cells / Q000276:immunology / Q000378:metabolism; D018418:Th2 Cells / Q000276:immunology / Q000378:metabolism; D015534:Trans-Activators / Q000096:biosynthesis*; D000099185:Interferon Regulatory Factor-8
chemical_list: D000911:Antibodies, Monoclonal; D017252:CD3 Complex; D002352:Carrier Proteins; D004268:DNA-Binding Proteins; D050856:Interferon Regulatory Factor-1; D050835:Interferon Regulatory Factors; D007371:Interferon-gamma; D008070:Lipopolysaccharides; D010750:Phosphoproteins; D011948:Receptors, Antigen, T-Cell; D012097:Repressor Proteins; D050794:STAT1 Transcription Factor; D015534:Trans-Activators; D000099185:Interferon Regulatory Factor-8; C494258:Irf1 protein, mouse; C494080:Stat1 protein, mouse