Molecular cloning and characterization of human bone morphogenic protein (BMP)-5 gene promoter.
The bone morphogenic proteins (BMPs) constitute a novel subfamily of the transforming growth factor type beta (TGF-beta) supergene family and play a critical role in modulating mesenchymal differentiation and inducing the processes of cartilage and bone formation. In this study we isolated the 5'-flanking region of the human BMP-5 gene. Nucleotide sequencing, primer extension, DNase I foot printing and functional analysis by transient expression showed that the cloned 1.5 kb promoter region contains two transcription start sites, a canonical TATA box (-17 approximately -12) and a number of consensus recognition sequences including GATA-1 (-582 approximately -576) and engrailed (-549 approximately -541).
Sakaue
M
M
Second Department of Pathology, Kobe University School of Medicine, Japan.
Kitazawa
S
S
Nishida
K
K
Kitazawa
R
R
Maeda
S
S
D001483:Base Sequence; D019485:Bone Morphogenetic Proteins; D003001:Cloning, Molecular; D004247:DNA; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D011506:Proteins / Q000235:genetics*; D014158:Transcription, Genetic
D019485:Bone Morphogenetic Proteins; D011506:Proteins; D004247:DNA