Genetic organization of a DNA-processing region required for mobilization of a non-self-transmissible plasmid, pEC3, isolated from Erwinia carotovora subsp. carotovora.
A non-self-transmissible multiple-copy plasmid, pEC3, isolated from the phytopathogenic bacterium, Erwinia carotovora subsp. carotovora, can be mobilized by an IncP-type plasmid. The hybrid plasmid vector, pETC3, constructed from pEC3 by fusion to markers conferring TcR and CmR, was transferred by conjugation from Escherichia coli (Ec) to various genera of Enterobacteriaceae and to other genera of Gram(-) bacteria which included Xanthomonas, Agrobacterium and Rhizobium. Deletion analysis and successive subcloning of pEC3 revealed that a cis-acting locus, oriT and a trans-acting locus, mob, were involved in mobilization of pEC3. Five open reading frames (ORFs) were found in the mob region, of which four were identified as mobA, B, C and D. The mobA gene overlapped with mobC, B, D and ORF1 that were transcribed polycistronically from upstream from mobC. The nature of the four products of mob genes, MobA, B, C and D, was verified by use of the T7 promoter system in Ec.
Nomura
N
N
Department of Fermentation Technology, Faculty of Engineering, Hiroshima University, Japan.
Yamashita
M
M
Murooka
Y
Y
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics; D017123:Bacteriophage T7 / Q000235:genetics; D001483:Base Sequence; D003227:Conjugation, Genetic / Q000235:genetics*; D004352:Drug Resistance, Microbial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D029968:Escherichia coli Proteins; D005798:Genes, Bacterial; D006090:Gram-Negative Bacteria / Q000235:genetics; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D016973:Pectobacterium carotovorum / Q000235:genetics*; D010957:Plasmids / Q000235:genetics* / Q000302:isolation & purification; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000235:genetics; D013045:Species Specificity; D015534:Trans-Activators / Q000235:genetics
D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; C099206:MobB protein, bacteria; C099207:MobC protein, bacteria; D011994:Recombinant Proteins; D015534:Trans-Activators; C095754:mobA protein, E coli; C099208:mobilization protein MobD, bacteria