In vivo role of IL-10 and IL-12 during development of Sjögren's syndrome in MRL/lpr mice.
Expression of local cytokine genes including interleukin 10 (IL-10) and IL-12 was analyzed in the salivary gland tissues of MRL/lpr mice with Sjögren's syndrome. We demonstrate a significant role of IL-10 and IL-12 in vivo during development of Sjögren's syndrome in MRL/lpr mice. IL-10 mRNA expression was detected before the onset of disease and was upregulated during the course of autoimmune sialadenitis by RT-PCR. A predominant level of expression of IL-12 mRNA was also detected earlier in the proinflammatory stage of autoimmune sialadenities. Moreover, MHC class II (I-Ak) mRNA was detected before the onset of inflammatory lesions until older ages in the salivary glands of MRL/lpr mice. These results suggest that endogenous IL-10 and IL-12 may play important roles on immune-mediated destruction of the salivary glands during development of organ-specific autoimmunity in MRL/lpr mice.
Yanagi
K
K
Department of Pathology, Tokushima University School of Dentistry, Japan.
Haneji
N
N
Hamano
H
H
Takahashi
M
M
Higashiyama
H
H
Hayashi
Y
Y
D000818:Animals; D001327:Autoimmune Diseases / Q000235:genetics / Q000503:physiopathology*; D001483:Base Sequence; D004195:Disease Models, Animal; D018450:Disease Progression; D005260:Female; D005786:Gene Expression Regulation; D000949:Histocompatibility Antigens Class II / Q000096:biosynthesis / Q000235:genetics; D016753:Interleukin-10 / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D018664:Interleukin-12 / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008817:Mice, Mutant Strains; D008969:Molecular Sequence Data; D009928:Organ Specificity; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger; D012469:Salivary Glands / Q000378:metabolism* / Q000473:pathology; D012793:Sialadenitis / Q000209:etiology / Q000235:genetics / Q000503:physiopathology; D012859:Sjogren's Syndrome / Q000235:genetics / Q000503:physiopathology*; D013154:Spleen / Q000378:metabolism / Q000473:pathology; D019014:fas Receptor / Q000235:genetics / Q000502:physiology*
D000949:Histocompatibility Antigens Class II; D012333:RNA, Messenger; D019014:fas Receptor; D016753:Interleukin-10; D018664:Interleukin-12