title: VLA-5-mediated interaction with fibronectin induces cytokine production by human chondrocytes.
abstract: Adhesion molecules of the integrin family, including very late activation antigens (VLA), have been implicated in various cellular functions. In this study, we investigated the contribution of integrin-mediated interaction with ECM proteins to the cytokine gene expression in human chondrocytes. Human articular chondrocytes expressed VLA-1, -2, -3 and -5 on the cell surface, and could adhere to various ECM proteins, especially to fibronectin (FN). Furthermore, the production of GM-CSF and IL-6 was potently induced by culturing chondrocytes on immobilized FN. This stimulative effect of FN was completely inhibited by an anti-integrin alpha 5 chain mAb, as well as by anti-integrin beta 1 chain mAbs. These results indicate an important role of the VLA-5-mediated interaction with FN in regulating inflammatory cytokine production by human articular chondrocytes.
authors.affiliation: Department of Orthopedic Surgery, Juntendo University School of Medicine, Tokyo, Japan.
authors.forename: I
authors.initials: I
authors.lastname: Yonezawa
authors.forename: K
authors.initials: K
authors.lastname: Kato
authors.forename: H
authors.initials: H
authors.lastname: Yagita
authors.forename: Y
authors.initials: Y
authors.lastname: Yamauchi
authors.forename: K
authors.initials: K
authors.lastname: Okumura
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D002358:Cartilage, Articular / Q000276:immunology* / Q000378:metabolism; D002448:Cell Adhesion; D002478:Cells, Cultured; D003094:Collagen / Q000494:pharmacology; D016207:Cytokines / Q000096:biosynthesis*; D016326:Extracellular Matrix Proteins / Q000494:pharmacology; D005353:Fibronectins / Q000494:pharmacology*; D015870:Gene Expression; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000096:biosynthesis; D006801:Humans; D016023:Integrins / Q000032:analysis / Q000096:biosynthesis*; D015850:Interleukin-6 / Q000096:biosynthesis; D046911:Macromolecular Substances; D051379:Mice; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D051381:Rats; D017469:Receptors, Fibronectin / Q000276:immunology / Q000502:physiology*
chemical_list: D000911:Antibodies, Monoclonal; D016207:Cytokines; D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D016023:Integrins; D015850:Interleukin-6; D046911:Macromolecular Substances; D012333:RNA, Messenger; D017469:Receptors, Fibronectin; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D003094:Collagen