In vitro evaluation of the cytotoxicity of calcium hydroxide-based root canal sealers.
The cytotoxicity of three calcium hydroxide-containing root canal sealers (Sealapex, CRCS and Apexit) was tested by using L929 and BHK 21/C13 cells. After setting for 24 h, the sealers were covered with cell suspension. Cytotoxicity was determined by a quantitative technique at 24 h, 48 h and 72 h. All the sealers were found to be cytotoxic. Sealapex showed the highest cytotoxicity, causing a significant decrease in cell density. CRCS was less toxic than Sealapex and more toxic than Apexit. Apexit proved to be the least toxic material.
Beltes
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P
Department of Dental Pathology and Therapeutics, Aristotle University Theagenion Cancer Institute, Thessaloniki, Greece.
Koulaouzidou
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E
Kotoula
V
V
Kortsaris
A H
AH
D000818:Animals; D002126:Calcium Hydroxide / Q000633:toxicity*; D002478:Cells, Cultured; D006224:Cricetinae; D005069:Evaluation Studies as Topic; D005347:Fibroblasts / Q000187:drug effects; D007668:Kidney / Q000166:cytology; D007739:L Cells; D051379:Mice; D012387:Root Canal Filling Materials / Q000633:toxicity*; D012459:Salicylates / Q000633:toxicity; D015034:Zinc Oxide / Q000633:toxicity
C074418:Apexit; D012387:Root Canal Filling Materials; D012459:Salicylates; C055601:Sealapex; C075563:Calcibiotic Root Canal Sealer; D002126:Calcium Hydroxide; D015034:Zinc Oxide