title: [Dietary fiber and pectin fractions of Beta vulgaris var. conditiva].
abstract: The alcohol-insoluble substance (AIS) from red beet (Beta vulgaris var. conditiva Alef.) (3.31% of the edible substance) was extracted sequentially with water, ammonium oxalate, 0.05 N HCl and 0.05 N NaOH. Accordingly 3.93 g, about 0.8 g, 2.96 g resp. 3.80 g galacturonan/100 g AIS were extracted with this procedure. These pectin extracts were purified as Cu2 +-salts and fractionated into a water-soluble and a water-insoluble part. The composition of neutral monosaccharide units was estimated in the fractions. Gal, Ara and Glc dominated; Xyl, Rha and Man were also present but in smaller amounts. A higher GalA content was found in the soluble fractions (with the exception of the alkali extract). Pectins from red beet are middle- or low-esterified and partially acetylated. The composition of the AIS and of the residue after pectin extraction (RE) was determined (14.6 resp. 9.5% pectin; 10,6 resp. 17.6% protein; 58.7 resp. 64.9% total polysaccharides). In the AIS 23.3% soluble and 54.7% insoluble dietary fiber were estimated, whereas in RE 15.3 resp. 54.7% were found (enzymatic method). Following dietary fiber fractions were determined by the detergent method for both preparations: 39.0 resp. 52.7% NDF; 6.3 resp. 4.5% NDF filtrate; 23.6 resp. 41.8% ADF; 1.2 resp. 1.8% lignin. The water binding capacity decreased from 19.85 g water (AIS) to 11. 53 g water (RE) related to 1 g AUS. From these just 50% were found in the NDF fractions and about 13% in the ADF fractions. Alterations of the grown biological structures during pectin extraction and dietary fiber analysis (detergent method) were investigated by scanning electron microscopy.
authors.affiliation: Deutsches Institut für Ernährungsforschung Potsdam-Rehbrücke, Bergholz-Rehbrücke, Germany.
authors.forename: G
authors.initials: G
authors.lastname: Dongowski
mesh_terms: D002849:Chromatography, Gas / Q000379:methods; D003300:Copper; D003902:Detergents; D004043:Dietary Fiber / Q000032:analysis*; D007202:Indicators and Reagents; D008855:Microscopy, Electron, Scanning; D009005:Monosaccharides / Q000032:analysis; D010368:Pectins / Q000032:analysis* / Q000737:chemistry; D014675:Vegetables / Q000737:chemistry* / Q000254:growth & development / Q000648:ultrastructure
chemical_list: D003902:Detergents; D004043:Dietary Fiber; D007202:Indicators and Reagents; D009005:Monosaccharides; D003300:Copper; D010368:Pectins