A modified Coomassie blue staining of proteins in polyacrylamide gels with Bismark brown R.
A rapid and sensitive protein staining method employing a mixed dye technique has been developed. Solutions of Coomassie brilliant blue R-250 (CB, 0.2%) and Bismark brown R (BBR, 0.05%) were mixed in the volume ratio of 1:0.75 for staining (final molar ratio, 4.5:1). In this staining, BBR inhibits the binding of CB to gel matrix by forming ion-pairing complex with excess CB; therefore, it reduces staining/destaining times and also enhances the staining effect of CB on protein band. As a result, the mixed dye staining enables complete staining/destaining within 20/25 min and detection of up to 25 ng of bovine serum albumin.
Choi
J K
JK
College of Pharmacy, Chonnam National University, Kwangju, 500-757, Korea.
Yoon
S H
SH
Hong
H Y
HY
Choi
D K
DK
Yoo
G S
GS
D000818:Animals; D001391:Azo Compounds; D002417:Cattle; D004396:Coloring Agents; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D011506:Proteins / Q000032:analysis*; D012710:Serum Albumin, Bovine / Q000032:analysis; D013997:Time Factors
D001391:Azo Compounds; D004396:Coloring Agents; D011506:Proteins; D012710:Serum Albumin, Bovine; C032806:Bismark Brown