PCR DNA typing of stamps: evaluation of the DNA extraction.
Today, the PCR analysis of DNA from the saliva deposited on a stamp of an anonymous letter can lead to an identification. However, this analysis still involves problems, and DNA extraction can be particularly difficult. A comparative study of two DNA extraction methods with two categories of postage stamps was carried out and a purification process was tested. This study shows that the extraction with phenol/chloroform gives much better results than Chelex extraction. A purification process such as the use of Centricon 100 microconcentrators is recommended when an inhibition of PCR is present. This operation, however, results in a large loss of material.
Fridez
F
F
Institut de Police Scientifique et de Criminologie, University of Lausanne, Switzerland.
Coquoz
R
R
D004247:DNA / Q000032:analysis* / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D005554:Forensic Medicine; D006801:Humans; D010678:Philately; D016133:Polymerase Chain Reaction; D012463:Saliva / Q000737:chemistry*
D004247:DNA