SINE sequences detect DNA fingerprints in salmonid fishes.
DNA probes homologous to two previously described salmonid short interspersed nuclear elements (SINEs) detected DNA fingerprint patterns in 14 species of salmonid fishes. The probes showed more homology to some species than to others and little homology to three nonsalmonid fishes. The DNA fingerprint patterns derived from the SINE probes are individual-specific and inherited in a Mendelian manner. Probes derived from different regions of the same SINE detect only partially overlapping banding patterns, reflecting a more complex SINE structure than has been previously reported. Like the human Alu sequence, the SINEs found in salmonids could provide useful genetic markers and primer sites for PCR-based techniques. These elements may be more desirable for some applications than traditional DNA fingerprinting probes that detect tandemly repeated arrays.
Spruell
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Department of Zoology, Washington State University, Pullman 99164-4236, USA.
Thorgaard
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GH
D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D016172:DNA Fingerprinting; D005819:Genetic Markers; D006801:Humans; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D017683:Oncorhynchus / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid; D012487:Salmonidae / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
D005819:Genetic Markers; D015345:Oligonucleotide Probes