Use of follicle-stimulating hormone (FSH) to increase the in vitro fertilization (IVF) efficiency of mice.
OBJECTIVE
The possibility of increasing the efficiency of an in vitro fertilization system (IVF) for Swiss OF1 mice was studied. The experimental protocol proposed analyzed the use of FSH as a superovulatory inducing hormone in comparison to traditional PMSG treatment. At the same time, the quality of IVF-derived embryos was evaluated both in vitro, with culture in CZB medium and fixation in advanced stages of development, and in vivo, by transfer to female recipients.


RESULTS
Treatment with FSH induced a much higher ovulation number compared PMSG (64.26 vs 33.85; P < 0.01). With this gonadotropin, IVF provided a positive tendency to normal fecundation (67.76 vs 64.72; P < 0.1) and a much lower index of abnormal division in embryos (10.57 vs 15.11; P < 0.05). The viability of embryos obtained from donors treated with hormones was similar, although differences did exist regarding embryo origin: those obtained following natural fertilization showed a higher developmental capacity from in vivo (P < 0.05) and in vivo (P < 0.05).


CONCLUSIONS
We conclude that FSH is an improved superovulation alternative treatment in comparison to PMSG for IVF. It provides a higher number of embryos with the same in vitro and in vivo viability as those obtained from PMSG.
Muñoz
I
I
Department of Patología Animal (Reproducción), Facultad de Veterinaria, Universidad de Zaragoza, Spain.
del Niño Jesus
A
A
Josa
A
A
Espinosa
E
E
Gil
I
I
D000818:Animals; D001755:Blastocyst; D004624:Embryo Transfer; D004622:Embryo, Mammalian / Q000166:cytology; D005314:Embryonic and Fetal Development; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D005640:Follicle Stimulating Hormone / Q000494:pharmacology*; D006064:Gonadotropins, Equine / Q000494:pharmacology; D008297:Male; D051379:Mice; D013480:Superovulation / Q000187:drug effects*
D006064:Gonadotropins, Equine; D005640:Follicle Stimulating Hormone