title: Recombinant insulin-like growth factor-1 modulates aggregation in human platelets via extracellular calcium.
abstract: Insulin like growth factor-1 (IGF-1) potentiated aggregation of human platelets induced by thrombin-, collagen- and ADP in a dose-dependent manner over the range 30-300 nM. IGF-1 (100 nM) reduced EC50 values for thrombin, collagen and ADP-induced aggregation by 19.6%, 53.6% and 22.8% respectively. Potentiation by IGF-1 was dependent on the presence of extracellular Ca2+ and was inhibited by verapamil or nifedipine. Further, IGF-1 enhanced the elevation in free intraplatelet Ca2+ induced by the platelet agonists collagen and thrombin.
authors.affiliation: William Harvey Research Institute, London, U.K.
authors.forename: A S
authors.initials: AS
authors.lastname: Motani
authors.forename: E E
authors.initials: EE
authors.lastname: Anggård
authors.forename: G A
authors.initials: GA
authors.lastname: Ferns
mesh_terms: D000244:Adenosine Diphosphate / Q000494:pharmacology; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism*; D002118:Calcium / Q000097:blood* / Q000494:pharmacology; D003094:Collagen / Q000494:pharmacology; D004357:Drug Synergism; D004533:Egtazic Acid / Q000494:pharmacology; D005110:Extracellular Space / Q000378:metabolism; D006801:Humans; D007328:Insulin / Q000494:pharmacology; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D010974:Platelet Aggregation / Q000187:drug effects*; D011994:Recombinant Proteins / Q000494:pharmacology; D013268:Stimulation, Chemical; D013917:Thrombin / Q000494:pharmacology
chemical_list: D007328:Insulin; D011994:Recombinant Proteins; D004533:Egtazic Acid; D000244:Adenosine Diphosphate; D007334:Insulin-Like Growth Factor I; D003094:Collagen; D013917:Thrombin; D002118:Calcium