title: Integration host factor amplifies the induction of the aceBAK operon of Escherichia coli by relieving IclR repression.
abstract: A binding site for integration host factor (IHF) was identified upstream of the aceBAK promoter. Under inducing conditions, IHF activates aceB::lacZ expression by opposing IclR repression. In contrast, IHF has little effect on aceB::lacZ expression under repressing conditions. The ability of IHF to relieve repression under inducing but not repressing conditions allows this protein to amplify the induction of aceBAK.
authors.affiliation: Department of Biochemistry, University of Minnesota, Minneapolis 55455, USA.
authors.forename: E
authors.initials: E
authors.lastname: Resnik
authors.forename: B
authors.initials: B
authors.lastname: Pan
authors.forename: N
authors.initials: N
authors.lastname: Ramani
authors.forename: M
authors.initials: M
authors.lastname: Freundlich
authors.forename: D C
authors.initials: DC
authors.lastname: LaPorte
mesh_terms: D000085:Acetates / Q000378:metabolism; D001426:Bacterial Proteins / Q000378:metabolism* / Q000502:physiology*; D001483:Base Sequence; D001665:Binding Sites; D004269:DNA, Bacterial / Q000378:metabolism; D004274:DNA, Recombinant; D004268:DNA-Binding Proteins / Q000378:metabolism*; D004790:Enzyme Induction; D004794:Enzyme Repression; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D005947:Glucose / Q000378:metabolism; D034982:Integration Host Factors; D008969:Molecular Sequence Data; D009876:Operon; D011401:Promoter Regions, Genetic / Q000235:genetics; D012097:Repressor Proteins / Q000502:physiology*; D014157:Transcription Factors; D001616:beta-Galactosidase / Q000235:genetics
chemical_list: D000085:Acetates; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004274:DNA, Recombinant; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; D034982:Integration Host Factors; D012097:Repressor Proteins; D014157:Transcription Factors; C067479:IclR protein, E coli; D001616:beta-Galactosidase; D005947:Glucose