title: Cloning and characterization of the Saccharomyces cerevisiae C-22 sterol desaturase gene, encoding a second cytochrome P-450 involved in ergosterol biosynthesis.
abstract: The ERG5 gene from Saccharomyces cerevisiae was cloned by complementation of an erg5-1 mutation using a negative selection protocol involving screening for nystatin-sensitive transformants. ERG5 is the putative gene encoding the C-22 sterol desaturase required in ergosterol biosynthesis. The functional gene was localized to a 2.15-kb SacI-EcoRI DNA fragment containing an open reading frame of 538 amino acids (aa). ERG5 contains a 10-aa motif consistent with its role as a cytochrome P-450 (CyP450) enzyme and is similar to a number of mammalian CyP450 enzymes. Gene disruption demonstrates that ERG5 is not essential for cell viability.
authors.affiliation: Department of Biology, Indiana University-Purdue University at Indianapolis 46202, USA.
authors.forename: B A
authors.initials: BA
authors.lastname: Skaggs
authors.forename: J F
authors.initials: JF
authors.lastname: Alexander
authors.forename: C A
authors.initials: CA
authors.lastname: Pierson
authors.forename: K S
authors.initials: KS
authors.lastname: Schweitzer
authors.forename: K T
authors.initials: KT
authors.lastname: Chun
authors.forename: C
authors.initials: C
authors.lastname: Koegel
authors.forename: R
authors.initials: R
authors.lastname: Barbuch
authors.forename: M
authors.initials: M
authors.lastname: Bard
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D004875:Ergosterol / Q000096:biosynthesis*; D015870:Gene Expression; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000235:genetics*; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins; D017386:Sequence Homology, Amino Acid
chemical_list: D029701:Saccharomyces cerevisiae Proteins; D003577:Cytochrome P-450 Enzyme System; D010088:Oxidoreductases; C066577:ERG5 protein, S cerevisiae; D004875:Ergosterol