title: Envelope gene sequences of human T-lymphotropic virus type 1 in Taiwan.
abstract: Three major types of HTLV-1 had been proposed, the Melanesian type, the Zairian type, and the cosmopolitan type, which was further divided into subtypes A, B and C, according to the phylogenetic tree constructed from LTR sequences of current HTLV-isolates. In this study, the envelope gene sequences of HTLV-1 from 9 Taiwanese were analyzed. Based on the phylogenetic tree constructed by unweighted pair group method and the sequence homology analysis by GCG computer programs, the envelope gene sequences of HTLV-1 proviruses from these 9 Taiwanese belonged to subtype A or subtype B of the cosmopolitan type and were closely related to HTLV-1 from Japan. Twelve subtype-specific nucleotide variations were deduced from the comparison of complete or partial envelope gene sequences of 16 HTLV-1 isolates of known subtypes as well as those of 9 Taiwanese. These data provided the basis for subtyping the cosmopolitan type of HTLV-1 by amplification of envelope gene sequences and restriction fragment length polymorphism studies. A more extensive survey based upon this proposal was warranted.
authors.affiliation: Department of Oncology, National Taiwan University Hospital, Taipei.
authors.forename: M T
authors.initials: MT
authors.lastname: Lin
authors.forename: Y C
authors.initials: YC
authors.lastname: Chen
authors.forename: P J
authors.initials: PJ
authors.lastname: Chen
authors.forename: Y C
authors.initials: YC
authors.lastname: Yang
authors.forename: J L
authors.initials: JL
authors.lastname: Tang
authors.forename: J M
authors.initials: JM
authors.lastname: Wu
authors.forename: S E
authors.initials: SE
authors.lastname: Chuang
authors.forename: C S
authors.initials: CS
authors.lastname: Yang
mesh_terms: D001483:Base Sequence; D017931:DNA Primers; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D015752:Genes, env; D014644:Genetic Variation; D015490:HTLV-I Infections / Q000453:epidemiology; D015368:Human T-lymphotropic virus 1 / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D007564:Japan; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D015995:Prevalence; D012689:Sequence Homology, Nucleic Acid; D012984:Software; D013624:Taiwan / Q000453:epidemiology
chemical_list: D017931:DNA Primers; D004279:DNA, Viral