Mechanical stress eliminates the effects of plasma from patients with preeclampsia on endothelial cells.
OBJECTIVE
Our purpose was to determine whether mechanical deformation alters in vitro effects of plasma from patients with preeclampsia on endothelial cell function to produce a paradigm similar to the in vivo disease state.


METHODS
The effects of 2% plasma from 12 patients with preeclampsia and 12 normal pregnant women on prostacyclin, nitric oxide, and endothelin production by cultured endothelial cells were measured in the presence or absence of cyclic stretch and laminar shear stress.


RESULTS
In the absence of mechanical stress plasma from patients with preeclampsia resulted in greater prostacyclin and nitric oxide production (but no change in endothelin production) compared with plasma from normal pregnant women. Cyclic stretch did not affect prostacyclin or endothelin production but produced similar increases in nitric oxide production in cells exposed to plasma from the two groups. Shear stress markedly increased prostacyclin and nitric oxide production (but did not alter endothelin production). In the presence of shear stress there were no differences in production rates of nitric oxide or prostacyclin between cells exposed to plasma from the two groups.


CONCLUSIONS
Shear stress alters the effects of plasma on endothelial cells. This is an important caveat in the interpretation of previous static in vitro culture studies and may partially explain the dichotomy between in vitro studies and in vivo findings in preeclampsia.
Baker
P N
PN
Magee-Womens Research Institute, University of Pittsburgh, PA 15213, USA.
Stranko
C P
CP
Davidge
S T
ST
Davies
P S
PS
Roberts
J M
JM
D000328:Adult; D000818:Animals; D001769:Blood; D002417:Cattle; D002478:Cells, Cultured; D003331:Coronary Vessels; D003470:Culture Media; D016232:Endothelins / Q000096:biosynthesis; D004730:Endothelium, Vascular / Q000378:metabolism*; D011464:Epoprostenol / Q000096:biosynthesis; D005260:Female; D006801:Humans; D009569:Nitric Oxide / Q000096:biosynthesis; D011225:Pre-Eclampsia / Q000097:blood*; D011247:Pregnancy; D013314:Stress, Mechanical
D003470:Culture Media; D016232:Endothelins; D009569:Nitric Oxide; D011464:Epoprostenol