Comparative analysis of ribonuclease P RNA structure in Archaea.
Although the structure of the catalytic RNA component of ribonuclease P has been well characterized in Bacteria, it has been little studied in other organisms, such as the Archaea. We have determined the sequences encoding RNase P RNA in eight euryarchaeal species: Halococcus morrhuae, Natronobacterium gregoryi, Halobacterium cutirubrum, Halobacteriurn trapanicum, Methanobacterium thermoautotrophicum strains deltaH and Marburg, Methanothermus fervidus and Thermococcus celer strain AL-1. On the basis of these and previously available sequences from Sulfolobus acidocaldarius, Haloferax volcanii and Methanosarcina barkeri the secondary structure of RNase P RNA in Archaea has been analyzed by phylogenetic comparative analysis. The archaeal RNAs are similar in both primary and secondary structure to bacterial RNase P RNAs, but unlike their bacterial counterparts these archaeal RNase P RNAs are not by themselves catalytically proficient in vitro.
Haas
E S
ES
Department of Microbiology, North Carolina State University, Raleigh 27695, USA.
Armbruster
D W
DW
Vucson
B M
BM
Daniels
C J
CJ
Brown
J W
JW
D001105:Archaea / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004722:Endoribonucleases / Q000235:genetics*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics*; D016337:RNA, Catalytic / Q000235:genetics*; D043262:Ribonuclease P; D012689:Sequence Homology, Nucleic Acid
D012329:RNA, Bacterial; D016337:RNA, Catalytic; D004722:Endoribonucleases; D043262:Ribonuclease P