title: Sequential determinations of Blomia tropicalis allergens in mattress and floor dust samples in a tropical city.
abstract: BACKGROUND
  The mite species Blomia tropicalis is commonly found in tropical and subtropical regions, and it is an important source of allergen in the city of Cartagena, Columbia.
  
  
  OBJECTIVE
  The study was designed to determine seasonal allergen levels of B. tropicalis in homes of patients with asthma and mite allergy.
  
  
  METHODS
  Dust samples from mattresses and floors in 20 homes were collected on a monthly basis for 1 year. Outdoor temperature, relative humidity, and rainfall were recorded. RAST inhibition was performed on extracts of dust samples. Allergen levels were compared with variations in climate.
  
  
  RESULTS
  B. tropicalis allergens were detected in all mattress samples. More than 50% RAST inhibition was detected in 30% of mattress samples and in 4.3% of floor samples, reflecting a high concentration of allergen. Significant correlations were only found between allergen levels and absolute humidity.
  
  
  CONCLUSIONS
  Levels of B. tropicalis allergen fluctuated minimally in Cartagena, Colombia.
authors.affiliation: Institute of Immunological Research, University of Cartagena, Columbia.
authors.forename: L
authors.initials: L
authors.lastname: Puerta
authors.forename: E
authors.initials: E
authors.lastname: Fernãndez-Caldas
authors.forename: D
authors.initials: D
authors.lastname: Mercado
authors.forename: R F
authors.initials: RF
authors.lastname: Lockey
authors.forename: L R
authors.initials: LR
authors.lastname: Caraballo
mesh_terms: D000485:Allergens / Q000032:analysis*; D000818:Animals; D001512:Bedding and Linens; D004391:Dust; D006801:Humans; D006813:Humidity; D008925:Mites / Q000276:immunology*; D012621:Seasons; D014329:Tropical Climate; D014505:Urban Population
chemical_list: D000485:Allergens; D004391:Dust