Isolation and characterization of 45 polymorphic microsatellites from the bovine genome.
A small-insert bovine genomic library was constructed in pBluescript II SK(+) and enriched for microsatellites by selective rescue of single-stranded pBluescript DNA carrying (CA)n/(TG)n tandem repeats. Approximately 50% of the clones in the enriched library contained (CA)n repeats or CA-rich sequences. Sequencing of clones selected for (CA)n repeats resulted in the identification and characterization of 45 (CA)n polymorphic microsatellites. Genotyping in 9 large paternal half-sib families indicated that 40 of these microsatellite markers exhibit autosomal Mendelian inheritance. The numbers of alleles range from 2 to 18, with an average of 6.3 per locus. The polymorphic microsatellite markers we have identified and characterized will contribute to the construction of a high-resolution linkage map of bovine genome.
Ma
R Z
RZ
Department of Animal Sciences, University of Illinois at Urbana-Champaign 61801, USA.
Russ
I
I
Park
C
C
Heyen
D W
DW
Beever
J E
JE
Green
C A
CA
Lewin
H A
HA
D000818:Animals; D001483:Base Sequence; D002417:Cattle / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D018900:Dinucleotide Repeats; D005260:Female; D015698:Genomic Library; D008297:Male; D018895:Microsatellite Repeats; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic
D017931:DNA Primers