Role of membrane fluidity in Epstein Barr virus (EBV) infectivity on Akata cell line.
Infection of Epstein Barr virus (EBV) to its host cells is initiated by the attachment of the glycoprotein gp 350/220 to the CR2 molecule. We used the sensitivity at the polar environment of the fluorescent probe Laurdan to study the membrane viscosity distribution from single leaving cells on two lymphoid cell lines Raji and Akata. Lipid analysis on both cells line demonstrated a lower cholesterol to phospholipid molar ratio on Akata than Raji cells. Cell fluidity analysis by Laurdan generalized emission polarization (GP) or by DPH polarization, indicated that membrane viscosity of Akata was lower than Raji cells. This difference was correlated to the increased susceptibility of Akata cells in expressing EBV early antigens (EA) after EBV superinfection.
Pozzi
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Dipartimento di Medicina Sperimentale e Patologia, Università 'La Sapienza' Roma, Italy.
Lisi
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A
Lanzilli
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G
Grimaldi
S
S
D015081:2-Naphthylamine / Q000031:analogs & derivatives; D002460:Cell Line; D002462:Cell Membrane / Q000502:physiology*; D002784:Cholesterol / Q000378:metabolism; D005456:Fluorescent Dyes; D004854:Herpesvirus 4, Human / Q000472:pathogenicity / Q000502:physiology*; D006801:Humans; D007848:Laurates; D008560:Membrane Fluidity; D008561:Membrane Fusion; D008563:Membrane Lipids / Q000378:metabolism; D008856:Microscopy, Fluorescence; D010743:Phospholipids / Q000378:metabolism; D011493:Protein Kinase C / Q000378:metabolism; D012680:Sensitivity and Specificity; D013696:Temperature; D013816:Thermodynamics; D014783:Viscosity
D005456:Fluorescent Dyes; D007848:Laurates; D008563:Membrane Lipids; D010743:Phospholipids; D002784:Cholesterol; D015081:2-Naphthylamine; D011493:Protein Kinase C; C065580:laurdan