title: The effect of cytokine stimulation on IL-1 receptor mRNA expression by intestinal epithelial cells.
abstract: Interleukin-1 (IL-1) can induce intestinal epithelial cells (IEC) to produce several cytokines and acute phase proteins, suggesting that IEC may be important in a cytokine network at the intestinal mucosa to amplify the effects of IL-1 during an inflammatory response. However, little is known about the type of IL-1 receptor expressed by IEC and the effect of cytokines on the expression of these receptors. In this study, the expression by IEC of IL-1 receptor Type I (IL-1RI) and type II (IL-1RII) mRNA was examined by reverse transcriptase polymerase chain reaction. Both isolated rat IEC and the rat IEC-6 intestinal epithelial cell line were found to express mRNA for IL-1RI but not IL-1RII. Stimulation of the IEC-6 cells with IL-1beta or TNF-alpha down-regulated the expression of mRNA for IL-1RI at 24 hr, yet transforming growth factor-beta1 was found to have no effect. These results suggest a possible mechanism to limit the effect of IL-1 on IEC function during the mucosal inflammatory response by down-regulating the expression of the Type I IL-1 receptor.
authors.affiliation: Department of Biological Sciences, Binghamton University (SUNY), New York 13902-6000, USA.
authors.forename: D W
authors.initials: DW
authors.lastname: McGee
authors.forename: S J
authors.initials: SJ
authors.lastname: Vitkus
authors.forename: P
authors.initials: P
authors.lastname: Lee
mesh_terms: D000818:Animals; D002460:Cell Line; D015536:Down-Regulation / Q000187:drug effects*; D004848:Epithelium / Q000378:metabolism; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology*; D007413:Intestinal Mucosa / Q000378:metabolism*; D051379:Mice; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D051381:Rats; D011916:Rats, Inbred F344; D017472:Receptors, Interleukin-1 / Q000096:biosynthesis* / Q000145:classification / Q000235:genetics; D011994:Recombinant Proteins / Q000494:pharmacology; D013045:Species Specificity; D013154:Spleen / Q000166:cytology; D013552:Swine; D016212:Transforming Growth Factor beta / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
chemical_list: D007375:Interleukin-1; D012333:RNA, Messenger; D017472:Receptors, Interleukin-1; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; D014409:Tumor Necrosis Factor-alpha