title: Enhancing effects of epidermal growth factor on human squamous cell carcinoma motility and matrix degradation but not growth.
abstract: In order to ascertain the effects of epidermal growth factor (EGF) on human cancer invasion abilities, three cell lines of human oral squamous cell carcinoma were studied using a phagokinetic track assay and zymography. EGF (1-100 ng/ml) was found to inhibit the growth but enhance the random motility of all three cell lines in a concentration-dependent fashion. Exposure to EGF, dose-dependently, led to an increased production of urokinase-type plasminogen activator and M(r) 92 kD matrix metalloproteinase by the same cells. These results strongly suggest that EGF may promote human squamous cell carcinoma invasion and metastasis.
authors.affiliation: Department of Oral Surgery, Health Sciences University of Hokkaido, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Shibata
authors.forename: T
authors.initials: T
authors.lastname: Kawano
authors.forename: H
authors.initials: H
authors.lastname: Nagayasu
authors.forename: K
authors.initials: K
authors.lastname: Okumura
authors.forename: M
authors.initials: M
authors.lastname: Arisue
authors.forename: J
authors.initials: J
authors.lastname: Hamada
authors.forename: N
authors.initials: N
authors.lastname: Takeichi
authors.forename: M
authors.initials: M
authors.lastname: Hosokawa
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D002455:Cell Division; D002465:Cell Movement / Q000187:drug effects; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D005109:Extracellular Matrix / Q000378:metabolism; D006801:Humans; D008666:Metalloendopeptidases / Q000378:metabolism; D014407:Tumor Cells, Cultured; D014568:Urokinase-Type Plasminogen Activator / Q000378:metabolism
chemical_list: D004815:Epidermal Growth Factor; D014568:Urokinase-Type Plasminogen Activator; D008666:Metalloendopeptidases