title: Generation of VV-hemorphin-7 from globin by peritoneal macrophages.
abstract: Bovine globin has been incubated with mice peritoneal macrophages in order to study its hydrolysis by lysosomal enzymes, among which chiefly cathepsin D. Analysis of resulting peptides, by reversed-phase high-performance liquid chromatography (RP-HPLC), showed the release of a bioactive peptide, VV-hemorphin-7. When a carboxyl proteinase inhibitor such as pepstatin A was added, no hemorphin was generated. Our results clearly demonstrated that VV-hemorphin-7 generation was principally due to cathepsin D. This study allowed us to hypothesize a possible pathway for in vivo hemorphins appearance from globin catabolism by macrophages.
authors.affiliation: Laboratoire de Génie Protéique et Cellulaire, Pôle Sciences et Technologies, La Rochelle, France.
authors.forename: N
authors.initials: N
authors.lastname: Dagouassat
authors.forename: I
authors.initials: I
authors.lastname: Garreau
authors.forename: F
authors.initials: F
authors.lastname: Sannier
authors.forename: Q
authors.initials: Q
authors.lastname: Zhao
authors.forename: J M
authors.initials: JM
authors.lastname: Piot
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002402:Cathepsin D / Q000378:metabolism; D002417:Cattle; D002478:Cells, Cultured; D005260:Female; D005914:Globins / Q000378:metabolism*; D006454:Hemoglobins / Q000737:chemistry / Q000378:metabolism*; D006868:Hydrolysis; D007976:Leupeptins / Q000494:pharmacology; D008247:Lysosomes / Q000201:enzymology; D017737:Macrophages, Peritoneal / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D010436:Pepstatins / Q000494:pharmacology; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism*; D011480:Protease Inhibitors / Q000494:pharmacology; D013047:Specific Pathogen-Free Organisms
chemical_list: D000596:Amino Acids; D006454:Hemoglobins; D007976:Leupeptins; D010436:Pepstatins; D010446:Peptide Fragments; D011480:Protease Inhibitors; C014195:Streptomyces pepsin inhibitor; C094394:VV-hemorphin-7; D005914:Globins; D002402:Cathepsin D; C032854:leupeptin; C031375:pepstatin