Evaluation of WHO monoclonal antibody kit for diagnosis of acute respiratory viral infections.
In 1990 and 1991, six laboratories located in the WHO Western Pacific Region (WPR) and South East Asian Region (SEAR) were selected, based on their experience in the immunofluorescence antibody technique (IFAT), to participate in the evaluation of a WHO monoclonal antibody (Mab) kit to detect respiratory syncytial (RS) virus, influenza A virus, influenza B virus, parainfluenza virus and adenovirus. Despite differences in the initial standardization procedures, the WHO monoclonal antibodies were found to be of high quality, sensitivity and specificity when tested on clinical specimens. The constant supply of affordable high quality reagents from WHO would enable their use in clinical virological laboratories in the developing countries as well as promote the utilization of IFAT as an adjunct to cell culture isolation in the diagnosis of acute respiratory viral infections.
Khairullah
N S
NS
Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
Lam
S K
SK
D000256:Adenoviridae / Q000302:isolation & purification*; D000911:Antibodies, Monoclonal; D001210:Asia, Southeastern; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D009980:Influenza A virus / Q000302:isolation & purification; D009981:Influenza B virus / Q000302:isolation & purification; D009975:Orthomyxoviridae / Q000302:isolation & purification*; D010139:Pacific Islands; D010252:Paramyxoviridae / Q000302:isolation & purification*; D011933:Reagent Kits, Diagnostic / Q000592:standards*; D018113:Respiratory Syncytial Virus, Human / Q000302:isolation & purification; D012141:Respiratory Tract Infections / Q000175:diagnosis* / Q000821:virology; D018108:Respirovirus / Q000302:isolation & purification; D014944:World Health Organization
D000911:Antibodies, Monoclonal; D011933:Reagent Kits, Diagnostic