Promoter regions of cysteine endopeptidase genes from legumes confer germination-specific expression in transgenic tobacco seeds.
Cysteine endopeptidases, SH-EP from Vigna mungo and EP-C1 from Phaseolus vulgaris, act to degrade seed storage protein during seed germination. Using transgenic tobacco plants, expression of SH-EP and promoter activity of the EP-C1 gene were analyzed in transgenic tobacco plants. The promoters of the two genes in tobacco seeds showed germination-specific activation, although post-translational processing of SH-EP and regulatory regions of promoter of the gene for EP-C1 were found to differ between leguminous seeds and transgenic tobacco seeds.
Yamauchi
D
D
Department of Biology, Tokyo Metropolitan University, Japan.
Terasaki
Y
Y
Okamoto
T
T
Minamikawa
T
T
D003546:Cysteine Endopeptidases / Q000235:genetics*; D007887:Fabaceae / Q000201:enzymology / Q000235:genetics*; D018506:Gene Expression Regulation, Plant; D017930:Genes, Reporter; D018525:Germination / Q000235:genetics*; D005966:Glucuronidase / Q000096:biosynthesis; D006651:Histocytochemistry; D030821:Plants, Genetically Modified; D010946:Plants, Medicinal; D010947:Plants, Toxic; D011401:Promoter Regions, Genetic; D014018:Tissue Distribution; D014026:Nicotiana / Q000235:genetics / Q000254:growth & development
D005966:Glucuronidase; D003546:Cysteine Endopeptidases