A short peptide eluted from the H-2Kb molecule of a polyomavirus-positive tumor corresponds to polyomavirus large T antigen peptide at amino acids 578 to 585 and induces polyomavirus-specific immunity.
A short peptide in complex with the H-2Kb molecule on PyRMA, a polyomavirus transfectant of the mouse lymphoma cell line RMA, was identified as a polyomavirus tumor-specific transplantation antigen. The peptide was obtained by affinity chromatography, acidic extraction, and reverse-phase high-pressure liquid chromatography (HPLC). In one HPLC fraction, a peptide sequence in which 5 of 8 amino acids, GKxGLxxA, corresponded to residues 578 to 585 of polyomavirus large T antigen was identified. In tumor rejection assays, we therefore tested three related synthetic peptides, corresponding to the octapeptide LT 578-585, GKTGLAAA; the nonapeptide LT 578-586, GKTGLAAAL; and the decapeptide LT 578-587, GKTGLAAALI. The octapeptide was found to give the most effective immunization against the outgrowth of the polyomavirus DNA-positive PyRMA tumor. However, none of the three peptides immunized against the original polyoma-virus-negative RMA line.
Berke
Z
Z
Division of Clinical Virology, Karolinska Institutet, Huddinge University Hospital, Sweden.
Palmer
S
S
Bergman
T
T
Wester
D
D
Svedmyr
J
J
Linder
S
S
Jornvall
H
H
Dalianis
T
T
D000595:Amino Acid Sequence; D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000276:immunology*; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D006183:H-2 Antigens / Q000276:immunology*; D006649:Histocompatibility Antigens / Q000276:immunology*; D007109:Immunity; D016399:Lymphoma, T-Cell; D051379:Mice; D008969:Molecular Sequence Data; D009368:Neoplasm Transplantation; D010446:Peptide Fragments / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification; D011120:Polyomavirus / Q000276:immunology*; D027601:Polyomavirus Infections / Q000276:immunology*; D013997:Time Factors; D014162:Transfection; D014407:Tumor Cells, Cultured; D014412:Tumor Virus Infections / Q000276:immunology*; D014765:Viral Vaccines / Q000276:immunology*
D000952:Antigens, Polyomavirus Transforming; D006183:H-2 Antigens; C032876:H-2Kb protein, mouse; D006649:Histocompatibility Antigens; D010446:Peptide Fragments; D014765:Viral Vaccines