title: A selenium supplement associated or not with vitamin E delays early renal lesions in experimental diabetes in rats.
abstract: Seventy rats were separated into five groups: one group of 12 was used as a control and received a purified diet, and four groups of streptozotocin-induced diabetic rats, totalling 58, were fed the same diet without or with selenium (Se) supplementation. Of the noncontrol rats, 14 were without supplementation (Group D), 14 were fed a Se-rich yeast diet (i.e., selenion) (Group DSel), 14 received selenomethionine (Group DSm), and 16 received selenomethionine + tocopherol acetate (Group DSmE). Supplementation with Se in all groups was 0.99 micromole/100g of diet and with tocopherol acetate was 0.145 micromole/100 g. All diabetic rats were mildly balanced by insulin. After 24 weeks of diet, plasma glucose tended to decrease in diabetic Se-supplemented groups DSmE > DSm > DSel versus Group D. In DSm and DSmE groups, plasma lipid peroxides also decreased compared with Group D, but this decrease reached significance only for DSmE (P < 0.01 for both TBARS and conjugated dienes). Plasma triglycerides also decreased in DSm and DSmE groups versus Group D (P < 0.01; P < 0.05, respectively). At the same time, Se increased significantly in kidneys of Groups DSel and DSm versus D and more weakly in Group DSmE, but in this case was associated with a large increase of vitamin E. These beneficial effects of selenium supplement and more so of selenium combined with vitamin E were associated with a protection of kidneys in diabetic rats which found expression in a significant correction of renal hyperfiltration (P < 0.05) and in a diminution of the number and severity of glomerular lesions (P < 0.0005).
authors.affiliation: National Institute of Health and Medical Research, Bron, France.
authors.forename: C
authors.initials: C
authors.lastname: Douillet
authors.forename: A
authors.initials: A
authors.lastname: Tabib
authors.forename: M
authors.initials: M
authors.lastname: Bost
authors.forename: M
authors.initials: M
authors.lastname: Accominotti
authors.forename: F
authors.initials: F
authors.lastname: Borson-Chazot
authors.forename: M
authors.initials: M
authors.lastname: Ciavatti
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000187:drug effects; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000150:complications* / Q000473:pathology; D003928:Diabetic Nephropathies / Q000473:pathology / Q000517:prevention & control*; D005527:Food, Fortified; D007678:Kidney Glomerulus / Q000473:pathology; D008297:Male; D009929:Organ Size; D051381:Rats; D017207:Rats, Sprague-Dawley; D012643:Selenium / Q000627:therapeutic use*; D013311:Streptozocin; D014810:Vitamin E / Q000627:therapeutic use*
chemical_list: D001786:Blood Glucose; D014810:Vitamin E; D013311:Streptozocin; D012643:Selenium