Determination of lactic acid and pyruvic acid in serum and cerebrospinal fluid by ion-exclusion chromatography with a bulk acoustic wave detector.
A chromatographic method base don a combination of ion-exclusion chromatography separation and bulk acoustic wave series piezoelectric quartz crystal detector quantification for the determination of pyruvic acid and lactic acid in serum and cerebrospinal fluid (CSF) was developed. The separation was carried out using a Shim-pak SCR-102H ion-exclusion column with phosphoric acid solution as eluent. The method shows an acceptable detection limit and anti-interference ability. Serum and CSF from healthy individuals and patient were analysed successfully.
Chen
P
P
New Materials Research Institute, Hunan University, Changsha, China.
Nie
L H
LH
Yao
S Z
SZ
D000162:Acoustics; D002845:Chromatography / Q000379:methods* / Q000706:statistics & numerical data; D006801:Humans; D007773:Lactates / Q000097:blood* / Q000134:cerebrospinal fluid*; D019344:Lactic Acid; D010756:Phosphoric Acids; D011773:Pyruvates / Q000097:blood* / Q000134:cerebrospinal fluid*; D019289:Pyruvic Acid; D012016:Reference Values; D012680:Sensitivity and Specificity; D012996:Solutions
D007773:Lactates; D010756:Phosphoric Acids; D011773:Pyruvates; D012996:Solutions; D019344:Lactic Acid; D019289:Pyruvic Acid; C030242:phosphoric acid