In vivo activation of T-cell induction into the primed phenotype and programmed cell death by staphylococcal enterotoxin B.
The authors demonstrate that SEB immunization activates V beta 8+ T cells and induces the acquisition of the primed phenotype as defined previously by low MEL-14 and high Pgp-1 expression. SEB-activated spleen CD4+ and CD8V beta 8+ T cells have different population dynamics and regulate the expression of MEL-14 and Pgp-1 differentially, suggesting that the SEB-MHC class II complex preferentially activates CD4V beta 8+ T cells. Interestingly, at day 3 after SEB immunization, V beta 8+ T cells expressing low, but not high, levels of MEL-14 undergo apoptosis, indicating that T-cell activation is a prerequisite for triggering programmed cell death. These results might help to trace antigen-reactive cells to the activated or primed pool, as well as to identify those cells which will undergo programmed cell death.
Aroeira
L S
LS
Department of Immunology and Oncology, Universidad Autonoma de Madrid, Spain.
Moreno
M C
MC
Martinez
C
C
D000818:Animals; D017209:Apoptosis / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology; D002453:Cell Cycle; D004768:Enterotoxins / Q000494:pharmacology*; D005260:Female; D008213:Lymphocyte Activation / Q000235:genetics / Q000502:physiology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010641:Phenotype; D013211:Staphylococcus aureus / Q000276:immunology; D018089:Superantigens / Q000494:pharmacology; D013601:T-Lymphocytes / Q000276:immunology*
D004768:Enterotoxins; D018089:Superantigens; C031818:enterotoxin B, staphylococcal