title: The N-terminal sequences (5-20) of thymosin beta 4 binds to monomeric actin in an alpha-helical conformation.
abstract: The relationship between the conformation of a peptide in solution and its interaction capacity is generally unclear. Trifluoroethanol (TFE), which stabilizes alpha-helical conformations, can be used to induce definite folding in synthetic peptides. The N-terminal part of thymosin beta 4, including the 5-20 sequences, is implicated in binding to monomeric actin. The corresponding peptide was synthesized and its conformation studied by CD. The peptide is unstructured in solution, and becomes folded at medium TFE concentrations, below 30%. In contrast, TFE does not significantly modify the conformation of monomeric actin which conserves its intrinsic properties, such as gelsolin interaction and DNase-I inactivation. We report here that the apparent affinity of the synthetic peptide to monomeric actin is increased by an order of magnitude in the presence of TFE, which implies that the peptide adopts a folded conformation needed for accurate interaction.
authors.affiliation: CNRS, UPR 9008 Centre de Recherches de Biochimie Macromoléculaire, INSERM U.249, Université de Montpellier I, France.
authors.forename: J
authors.initials: J
authors.lastname: Feinberg
authors.forename: F
authors.initials: F
authors.lastname: Heitz
authors.forename: Y
authors.initials: Y
authors.lastname: Benyamin
authors.forename: C
authors.initials: C
authors.lastname: Roustan
mesh_terms: D000199:Actins / Q000378:metabolism* / Q000648:ultrastructure; D000595:Amino Acid Sequence; D000818:Animals; D002942:Circular Dichroism; D018260:Gelsolin / Q000737:chemistry; D008840:Microfilament Proteins / Q000737:chemistry*; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry; D017433:Protein Structure, Secondary / Q000187:drug effects; D011817:Rabbits; D013050:Spectrometry, Fluorescence; D013947:Thymosin / Q000737:chemistry / Q000378:metabolism*; D014270:Trifluoroethanol / Q000494:pharmacology; D014364:Tryptophan / Q000737:chemistry; D000099201:Thymosin beta(4)
chemical_list: D000199:Actins; D018260:Gelsolin; D008840:Microfilament Proteins; D010446:Peptide Fragments; D013947:Thymosin; D014270:Trifluoroethanol; D014364:Tryptophan; D000099201:Thymosin beta(4)