title: Leishmania amazonensis: the Asian rhesus macaques (Macaca mulatta) as an experimental model for study of cutaneous leishmaniasis.
abstract: As a means of assessing the usefulness of the Rhesus macaque (Macaca mulatta) as a nonhuman primate model for studying cutaneous leishmaniasis, monkeys were infected with Leishmania amazonensis. Variation in the level of susceptibility was found; however, animals inoculated with 10(8) promastigotes provided consistent results as indicated by an earlier onset and/or larger size of lesions. Three monkeys, which had recovered from skin lesions, were challenge-infected using the same parasite strain/dose; although these animals remained susceptible to homologous infection, lesion size was smaller and healed faster than in the initial infection. The immunologic features during infection were assessed. Levels of IgM and IgG antibodies to promastigote antigens rose during active infection and then declined; immunoblot analyses indicated that numerous leishmanial antigens (predominately >30 kDa) were recognized. Delayed type hypersensitivity (DTH) responses and proliferative responses (PBL) developed during active infection and/or rechallenge. Circulating peripheral T cell subpopulations varied throughout the course of infection. Initially (6-8 weeks p.i.), CD4+ T cells appear to predominate; subsequently (15-21 weeks p.i.), an increase in CD8+ T cells was observed. Pathologic analyses indicated that lesions contained amastigotes with a mononuclear infiltrate of macrophages, lymphocytes, and plasma cells, and formation of tuberculoid-type granulomas. As the progression and resolution of leishmanial infection in rhesus macaques are very similar to those observed in humans, this primate model could be employed for elucidating the mechanisms of protective immunity in cutaneous leishmaniasis.
authors.affiliation: Departamento de Imunologia, Instituto Oswaldo Cruz, Rio de Janeiro, Brazil.
authors.forename: V F
authors.initials: VF
authors.lastname: Amaral
authors.forename: V A
authors.initials: VA
authors.lastname: Ransatto
authors.forename: F
authors.initials: F
authors.lastname: Conceicão-Silva
authors.forename: E
authors.initials: E
authors.lastname: Molinaro
authors.forename: V
authors.initials: V
authors.lastname: Ferreira
authors.forename: S G
authors.initials: SG
authors.lastname: Coutinho
authors.forename: D
authors.initials: D
authors.lastname: McMahon-Pratt
authors.forename: G
authors.initials: G
authors.lastname: Grimaldi
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D000953:Antigens, Protozoan; D015153:Blotting, Western; D004195:Disease Models, Animal; D004198:Disease Susceptibility; D005260:Female; D007111:Immunity, Cellular; D016773:Leishmaniasis, Cutaneous / Q000276:immunology / Q000473:pathology / Q000662:veterinary*; D008213:Lymphocyte Activation; D008253:Macaca mulatta / Q000469:parasitology*; D008297:Male; D012867:Skin / Q000473:pathology
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan