Significantly different fatty acid profiles in various phospholipid head groups of neuroblastoma cell lines.
We studied lipid profiles of hybrid cells derived from fusion of sympathetic ganglia and neuroblastoma cells (NCB 20, F 11) and also CHO (Chinese hamster ovary) cells. The proportion of saturated to unsaturated fatty acids changed significantly in different phospholipid fractions. We observed 35.4% of total fatty acids as saturated fatty acids in the phosphatidyl ethanolamine (PE) fraction of NCB-20 cell lines while 47.2% of total fatty acids were saturated in the phosphatidyl inositol (PI) fraction. In general, in neuroblastoma cell lines, we observed the lowest proportion of saturated fatty acids in the PE fraction while the other lipid fraction showed a much higher proportion of saturated fatty acids. On the other hand the PE fraction of a non neuronal cell line, CHO, showed 57.4% saturated fatty acids in the PE fraction. This significant difference in saturated to unsaturated fatty acid ratios in different phospholipid head groups may be linked to the different biological functions of those hybrid cells.
Dasgupta
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A
Department of Pathology, University of New Mexico School of Medicine, Albuqueraue 87106, USA.
Banerjee
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P
D000818:Animals; D016466:CHO Cells / Q000378:metabolism; D006224:Cricetinae; D005227:Fatty Acids / Q000378:metabolism*; D005231:Fatty Acids, Unsaturated / Q000378:metabolism; D006822:Hybrid Cells; D051379:Mice; D009447:Neuroblastoma / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism*; D051381:Rats; D014407:Tumor Cells, Cultured
D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated; D010743:Phospholipids