Novel properties of L-type polypeptide subunits in mouse ferritin molecules.
Properties of the L- and H-type polypeptide subunits forming ferritin 24-mer molecules in mice were investigated, using the products of in vitro transcription and translation from the two cloned genes, and recombinant ferritin molecules (H24L0 or H0L24) produced by transformation in Escherichia coli. Several different conditions for analytical electrophoresis reproducibly show that the relative migration position of the two mouse ferritin subunits is reversed from that reported for ferritin H- and L-subunits in all other mammals; since mouse and human H-polypeptides almost co-migrate, this unusual relative mobility is due largely to novel properties of the murine L-subunit. This unusual electrophoretic property of the mouse L-subunit has led to conflicting reports about the subunit composition of natural mouse ferritin. Here, we show that the single major electrophoretic band given by liver ferritin purified from mice having a short-term iron overload matches that produced by the genetically defined L-polypeptide and that some bona fide H-subunits are also detected. In conclusion, it is reasonable to assume that, when mouse ferritin samples will be analyzed under the same conditions as those described here, the slower species will correspond to the L-type subunit. However, when dealing with ferritin from species other than human or mouse, it should be kept in mind that upon electrophoretic analysis of ferritin polypeptide, the designation of an electrophoretic band as being H- or L-type subunits will be very uncertain without corroboration from genetic, immunological, or amino acid sequencing data.
Beaumont
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INSERM U409, Faculté de Medecine Xavier Bichat, 75870 Paris Cedex 18, France.
Torti
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Massover
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D000818:Animals; D001052:Apoferritins / Q000737:chemistry; D002478:Cells, Cultured; D018076:DNA, Complementary / Q000235:genetics; D005293:Ferritins / Q000737:chemistry*; D006801:Humans; D051379:Mice; D051381:Rats; D011994:Recombinant Proteins
D018076:DNA, Complementary; D011994:Recombinant Proteins; D005293:Ferritins; D001052:Apoferritins