ATP-dependent H+ -pump activity in inverted vesicles of Methanosarcina mazei Gö1 and characterization of membrane ATPase.
ATP-dependent H+ -pump activity was found in inverted vesicles of Methanosarcina mazei Gö1 by using acridine orange as a fluorescent probe. The H+ -pump activity specifically required both Mg and sulfite ions, but azide, an inhibitor of F0F1-ATPase, did not inhibit the activity. The membranes prepared from M. mazei also had an Mg-ATPase activity, and at least the presence of vacuolar-type ATPase was detected.
Inatomi
K I
KI
Advanced Technology R & D Center, Mitsubishi Electric Corporation, Hyogo, Japan. inatomi@bio.crl.melco.co.jp
D000165:Acridine Orange; D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000378:metabolism*; D000595:Amino Acid Sequence; D017301:Ca(2+) Mg(2+)-ATPase / Q000378:metabolism; D005456:Fluorescent Dyes; D008565:Membrane Proteins / Q000378:metabolism*; D017020:Methanosarcina / Q000201:enzymology*; D008969:Molecular Sequence Data; D017494:Proton Pumps / Q000378:metabolism*; D006180:Proton-Translocating ATPases / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
D005456:Fluorescent Dyes; D008565:Membrane Proteins; D017494:Proton Pumps; D000255:Adenosine Triphosphate; D000251:Adenosine Triphosphatases; D017301:Ca(2+) Mg(2+)-ATPase; D006180:Proton-Translocating ATPases; D000165:Acridine Orange