Increased proteolytic activity in lymphocytes from patients with early onset Alzheimer's disease.
The levels of calpains (m-calpain and mu-calpain) in peripheral blood lymphocytes from patients with Alzheimer's disease were determined via Western blotting. The Ca-dependent proteolytic activity and the calpastatin activity were estimated using incubation with exogenous substrate. Evidence was obtained for an increased Ca-dependent proteolytic activity in lymphocytes from patients with early onset Alzheimer's disease. There was also an increased level of membrane-bound mu-calpain in this group of patients. The observed changes may be caused by a general dysregulation of Ca homeostasis in peripheral cells of early onset Alzheimer victims.
Karlsson
J O
JO
Department of Anatomy and Cell Biology, Göteborg University, Sweden.
Blennow
K
K
Janson
I
I
Blomgren
K
K
Karlsson
I
I
Regland
B
B
Wallin
A
A
Gottfries
C G
CG
D017668:Age of Onset; D000368:Aged; D000544:Alzheimer Disease / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism; D002135:Calcium-Binding Proteins / Q000378:metabolism*; D002154:Calpain / Q000378:metabolism*; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism*; D008297:Male; D008875:Middle Aged
D002135:Calcium-Binding Proteins; C033668:calpastatin; D002154:Calpain; D002118:Calcium