title: Effect of progesterone and estradiol benzoate on superoxide dismutase activity in the brain of male rats.
abstract: The activities of mitochondrial, manganese-containing superoxide dismutase (MnSOD) and cytoplasmic, copper-zinc-containing superoxide dismutase (CuZnSOD) were measured in subcellular fractions of whole brain homogenates prepared from intact and gonadectomized (GDX) male rats, untreated or treated subcutaneously (sc) with a single dose of 2 mg progesterone (P) and/or 5 micrograms estradiol benzoate (EB). Neither MnSOD nor CuZnSOD was affected by the removal of the testes. Similarly, CuZnSOD activity was steady following systemic administration of P and/or EB to intact and GDX animals 2 h or 24 h prior to sacrifice. On the other hand, both P and EB suppressed MnSOD in the brain of either intact or GDX rats. These results suggest involvement of P and EB in the control of MnSOD activity in the brain of male rats.
authors.affiliation: Laboratory for Molecular Biology and Endocrinology, Vinća Institute for Nuclear Sciences, Belgrade, Yugoslavia.
authors.forename: S
authors.initials: S
authors.lastname: Pajović
authors.forename: Z S
authors.initials: ZS
authors.lastname: Saicić
authors.forename: M B
authors.initials: MB
authors.lastname: Spasić
authors.forename: V M
authors.initials: VM
authors.lastname: Petrović
authors.forename: J V
authors.initials: JV
authors.lastname: Martinović
mesh_terms: D000818:Animals; D001921:Brain / Q000187:drug effects / Q000201:enzymology*; D002474:Cell-Free System; D003593:Cytoplasm / Q000201:enzymology; D004958:Estradiol / Q000494:pharmacology*; D008297:Male; D009919:Orchiectomy; D011374:Progesterone / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism*
chemical_list: D011374:Progesterone; D004958:Estradiol; D013482:Superoxide Dismutase