title: Effect of heat-drug sequences on thermoenhancement and uptake of cis-DDP in human pharyngeal carcinoma.
abstract: To discover the point of maximum interactive effect, we examined the time sequence of high (above (42.5 degrees C) or low (below 42.5 degrees C) -hyperthermia and cis-diammine dichloroplatinum (II) (CDDP). Simultaneous or post-hyperthermic CDDP (0.5 micrograms/ml) treatment at 43 degrees C resulted in a slight synergistic effect, with thermoenhancement ratios (TER) of 1.42 or 1.38, respectively, and there was a significant increase CDDP uptake after both combinations compared with pre-hyperthermic CDDP treatment. However, at 42 degrees C, the maximal interaction (TER = 8.57) was obtained when KB cells were simultaneously heated with CDDP, there was also a significant increase of CDDP uptake by simultaneous procedures compared with pre or post-hyperthermic CDDP treatment. These results indicate that simultaneous or post-hyperthermic CDDP treatment for high-hyperthermia and simultaneous CDDP treatment for low-hyperthermia are the most effective means of CDDP thermochemotherapy with hyperthermia.
authors.affiliation: Department of Otolaryngology, Fukui Medical School, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Ohtsubo
authors.forename: H
authors.initials: H
authors.lastname: Saito
authors.forename: N
authors.initials: N
authors.lastname: Tanaka
authors.forename: I
authors.initials: I
authors.lastname: Noda
authors.forename: T
authors.initials: T
authors.lastname: Saito
authors.forename: E
authors.initials: E
authors.lastname: Kano
mesh_terms: D000970:Antineoplastic Agents / Q000493:pharmacokinetics / Q000494:pharmacology*; D016923:Cell Death / Q000187:drug effects / Q000502:physiology; D002945:Cisplatin / Q000493:pharmacokinetics / Q000494:pharmacology*; D003131:Combined Modality Therapy; D006801:Humans; D006979:Hyperthermia, Induced; D007624:KB Cells; D013696:Temperature
chemical_list: D000970:Antineoplastic Agents; D002945:Cisplatin