title: Explorative study of interleukin levels in the human cornea.
abstract: BACKGROUND
  The presence of interleukins has been demonstrated in the cornea and other ocular tissues. Although pathogenic mechanisms are unknown, interleukins seem to be involved in inflammatory disorders of the cornea. The present study was undertaken to analyse concentrations of interleukin-1 beta (IL-1 beta) and interleukin-6 (IL-6) in human corneas with various clinical diagnoses.
  
  
  METHODS
  Immediately after keratoplasty 127 explanted human corneas with various corneal diseases were snap frozen and cryosections were prepared for histological examination. Furthermore, the protein content was measured according to the method of Bradford and the concentration of IL-1 beta and IL-6 were determined using a specific immunosorbent test (ELISA).
  
  
  RESULTS
  It was found that IL-1 beta and IL-6 level were clearly higher in corneas with ulcerations and distinct inflammatory signs. Lower levels of both interleukins were found in corneas with a weak expression of inflammatory signs.
  
  
  CONCLUSIONS
  Keratitis, keratoconus with inflammatory signs, and ulcerating processes showed higher interleukin levels than corneas with non-inflammatory disorders like scar formation, corneal dystrophy and keratoconus. The results could show that, depending on the clinical diagnosis, the inflammatory status of the cornea may be evaluated by the interleukin levels determined in the corneal tissue.
authors.affiliation: Klinikum der RWTH Aachen, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Becker
authors.forename: S
authors.initials: S
authors.lastname: Salla
authors.forename: U
authors.initials: U
authors.lastname: Dohmen
authors.forename: C
authors.initials: C
authors.lastname: Redbrake
authors.forename: M
authors.initials: M
authors.lastname: Reim
mesh_terms: D003315:Cornea / Q000378:metabolism* / Q000601:surgery; D003316:Corneal Diseases / Q000378:metabolism / Q000601:surgery; D004797:Enzyme-Linked Immunosorbent Assay; D005136:Eye Proteins / Q000378:metabolism; D006801:Humans; D007375:Interleukin-1 / Q000378:metabolism*; D015850:Interleukin-6 / Q000378:metabolism*; D007634:Keratitis / Q000378:metabolism / Q000601:surgery; D015948:Keratoplasty, Penetrating
chemical_list: D005136:Eye Proteins; D007375:Interleukin-1; D015850:Interleukin-6