The mutation frequency of feline immunodeficiency virus enhanced by 3'-azido-3'-deoxythymidine.
We have developed a host range system to measure the mutation frequency of feline immunodeficiency virus (FIV), the feline homologue of human immunodeficiency virus type 1 (HIV-1). When wild-type FIV was grown in the presence of a known mutagen, 5-bromo-2'-deoxyuridine (BUdR), a dose-dependent increase of host range mutants was detected. Using this system, we have evaluated the effects of antiviral drugs upon the mutation frequency of FIV. Subinhibitory concentrations of 3'-azido-3'-deoxythymidine (AZT), the most common antiviral drug used in AIDS chemotherapy, increased the mutation frequency of FIV in a dose-dependent manner. Two other antivirals, 2',3'-dideoxyinosine (ddI) and 2'3'-dideoxycytidine (ddC), did not show this effect.
LaCasse
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Division of Biological Sciences, University of Montana, Missoula, 59812, USA.
Remington
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North
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D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D001973:Bromodeoxyuridine / Q000494:pharmacology; D002415:Cats; D002460:Cell Line; D016049:Didanosine / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005787:Gene Frequency / Q000187:drug effects; D016089:Immunodeficiency Virus, Feline / Q000187:drug effects* / Q000235:genetics; D007668:Kidney / Q000166:cytology / Q000821:virology; D009154:Mutation / Q000187:drug effects*; D014776:Virus Cultivation; D016047:Zalcitabine / Q000494:pharmacology; D015215:Zidovudine / Q000494:pharmacology*
D000998:Antiviral Agents; D015215:Zidovudine; D016047:Zalcitabine; D001973:Bromodeoxyuridine; D016049:Didanosine