Expression and the role of cathepsin H in human glioma progression and invasion.
Proteinases and their inhibitors may play a role in the development and progression of many cancers. Several studies suggested that lysosomal proteinases cathepsin B, L, and D may be involved in the malignant progression of some human neoplastic diseases. In this study, we determined the levels of cathepsin H in human glioma progression and the significance of cathepsin H in glioma cell invasion. Levels of cathepsin H antigen were found to be significantly higher in glioblastomas and anaplastic astrocytoma when compared with normal brain tissue and low-grade gliomas. Western blotting confirmed the presence of authentic cathepsin H with a doublet at 27 and 25 kDa in normal brain tissue and tumor samples. However, the intensity of the band increased significantly in glioblastoma samples. Cathepsin H antibody inhibited the invasion of glioblastoma cell lines through Matrigel invasion assay. These data suggest that the tumor-specific increase in antigen may be a useful independent marker of tumor progression in central nervous system neoplasms.
Sivaparvathi
M
M
Department of Neurosurgery, University of Texas M.D. Anderson Cancer Center, Houston 77030, USA.
Sawaya
R
R
Gokaslan
Z L
ZL
Chintala
S K
SK
Rao
J S
JS
Chintala
K S
KS
D000951:Antigens, Neoplasm / Q000096:biosynthesis / Q000378:metabolism; D015153:Blotting, Western; D001932:Brain Neoplasms / Q000201:enzymology* / Q000473:pathology*; D056655:Cathepsin H; D002403:Cathepsins / Q000096:biosynthesis / Q000378:metabolism*; D003546:Cysteine Endopeptidases; D018450:Disease Progression; D004797:Enzyme-Linked Immunosorbent Assay; D005910:Glioma / Q000201:enzymology* / Q000473:pathology*; D006801:Humans; D009361:Neoplasm Invasiveness
D000951:Antigens, Neoplasm; D002403:Cathepsins; D003546:Cysteine Endopeptidases; C534126:CTSH protein, human; D056655:Cathepsin H