title: On-line Raman spectroscopy of ribonucleotides preconcentrated by capillary isotachophoresis.
abstract: Normal Raman spectroscopy is used as an on-line detector for capillary isotachophoresis (ITP) of adenosine 5'-triphosphate, adenosine 5'-diphosphate, and adenosine 5'-monophosphate in phosphate buffers. Preconcentration is from a 1 x 10(-2) M phosphate buffer (pH 7.5) into a leading electrolyte of 0.1 M KCl or Na2SO4, with a terminating electrolyte of 0.1 M 4-morpholinepropane-sulfonic acid. The ribonucleotides are concentrated to above 10(-2) M at the detection window, allowing measurement of Raman spectra with 1 s integration, from starting concentrations of 5 x 10(-6) M or higher.
authors.affiliation: Department of Chemistry, University of Michigan, Ann Arbor 48109-1055, USA.
authors.forename: P A
authors.initials: PA
authors.lastname: Walker
authors.forename: W K
authors.initials: WK
authors.lastname: Kowalchyk
authors.forename: M D
authors.initials: MD
authors.lastname: Morris
mesh_terms: D000244:Adenosine Diphosphate / Q000032:analysis*; D000249:Adenosine Monophosphate / Q000032:analysis*; D000255:Adenosine Triphosphate / Q000032:analysis*; D019075:Electrophoresis, Capillary; D013059:Spectrum Analysis, Raman
chemical_list: D000249:Adenosine Monophosphate; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate