title: Reduced humoral immunity in uremic mice genetically selected for high antibody response.
abstract: Biozzi's Selection IV-A mice, genetically selected for 25 generations for high and low antibody response to sheep red blood cells (SRBC), 2-3 months old, were made uremic by subtotal nephrectomy and characterized for antibody production against the selection antigen. T cell activity was evaluated in vitro by lymphocyte proliferation and interleukin 2 (IL 2) production in response to the super antigen staphylococcal enterotoxin B (SEB). Total and IgM antibody titers (log2) were similar in uremic and non-uremic low responder mice (total antibody: 4.0 +/- 0.6 vs 3.6 +/- 0.6; IgG: 3.0 +/- 0.7 vs 2.4 +/- 0.4), while uremic high responders presented with non-uremic animals (total antibody: 10.8 +/- 1.6 vs 13.0 +/- 0.2; IgG: 10.3 +/- 1.5 vs 11.7 +/- 0.3). T cell proliferation and IL 2 production were similar in uremic and non-uremic groups after SEB stimulation. The results indicate a genetic effect on sensitivity to humoral immune response modulation by uremic status, with deficient antibody production despite a normal T cell proliferative response to mitogen stimulation in short-duration renal failure in high responder mice.
authors.affiliation: Laboratório de Fisiopatologia Renal, Faculdade de Medicina, Universidade de São Paulo, Brasil.
authors.forename: E B
authors.initials: EB
authors.lastname: Prado
authors.forename: W H
authors.initials: WH
authors.lastname: Cabrera
authors.forename: A L
authors.initials: AL
authors.lastname: Nicastri
authors.forename: M J
authors.initials: MJ
authors.lastname: Prado
authors.forename: P
authors.initials: P
authors.lastname: Léo
authors.forename: M
authors.initials: M
authors.lastname: Marcondes
authors.forename: M
authors.initials: M
authors.lastname: Siqueira
authors.forename: O C
authors.initials: OC
authors.lastname: Ibañez
mesh_terms: D000818:Animals; D000917:Antibody Formation / Q000235:genetics*; D051379:Mice; D008815:Mice, Inbred Strains; D013601:T-Lymphocytes / Q000502:physiology*; D014511:Uremia / Q000276:immunology*