Structural and immunochemical characterization of the type VIII group B Streptococcus capsular polysaccharide.
The type VIII capsular polysaccharide has been isolated and purified from a newly described strain of group B Streptococcus which is a leading cause of sepsis and neonatal meningitis in Japan. The polysaccharide contains D-glucose, D-galactose, L-rhamnose, and sialic acid in the molar ratio 1:1:1:1. By means of high resolution 1H nuclear magnetic resonance (1H NMR), 13C NMR, and homo- and heterocorrelated NMR, the repeating unit structure of the type VIII polysaccharide was delineated as the following, [formula: see text] Enzymatic studies established this polysaccharide as the first from which sialic acid, linked to a branched beta-D-galactopyranosyl residue, is known to be removed by bacterial neuraminidase.
Kogan
G
G
Institute for Biological Sciences, National Research Council of Canada, Ottawa, Ontario, K1A 0R6, Canada.
Uhrín
D
D
Brisson
J R
JR
Paoletti
L C
LC
Blodgett
A E
AE
Kasper
D L
DL
Jennings
H J
HJ
D000942:Antigens, Bacterial / Q000737:chemistry*; D016667:Bacterial Capsules / Q000737:chemistry* / Q000276:immunology*; D002240:Carbohydrate Sequence; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D012794:Sialic Acids; D013292:Streptococcus agalactiae / Q000737:chemistry* / Q000276:immunology*
D000942:Antigens, Bacterial; D012794:Sialic Acids