Evidence of involvement of cytotoxic antibodies directed against patients's HLA class II produced by transfused donor-derived B cells in post-transfusion graft-versus-host disease.
Post-transfusion graft-versus-host disease (PTGVHD) is one of the most severe side-effects of blood transfusion. To characterize the effector cells causing this disease, we cloned lymphocytes from a PTGVHD patient's peripheral blood. T-cell and B-cell clones were established, the origins of which were proven to be transfused donor lymphocytes. It was found that the B cells produced IgG that mediated complement-dependent cytotoxicity to the cells bearing the patient's HLA class II genotype. Our results suggest, for the first time, the involvement of B-cell-produced cytotoxic antibodies directed against patient's HLA class II in the pathogenesis of PTGVHD.
Nishimura
M
M
Department of Research, The Japanese Red Cross Central Blood Centre, Tokyo, Japan.
Uchida
S
S
Mitsunaga
S
S
Tokunaga
K
K
Tadokoro
K
K
Juji
T
T
D000906:Antibodies / Q000235:genetics / Q000627:therapeutic use*; D001402:B-Lymphocytes / Q000276:immunology*; D002999:Clone Cells; D003602:Cytotoxicity, Immunologic; D005260:Female; D005802:Genes, MHC Class II; D006086:Graft vs Host Disease / Q000276:immunology*; D006801:Humans; D008875:Middle Aged; D013601:T-Lymphocytes / Q000276:immunology*; D065227:Transfusion Reaction
D000906:Antibodies