Neurotensin stimulates neutrophil adherence to bronchial epithelial cells in vitro.
Neutrophils and neuropeptides have both been implicated in airway inflammation. We hypothesized that neurotensin, a neuropeptide found in the airways, would stimulate neutrophil adherence to bronchial epithelial cells. Adherence was assessed using 51Cr-labelled human neutrophils added to confluent monolayers of bovine bronchial epithelial cells. Neurotensin added to bronchial epithelial cells produced a time-and concentration-dependent increase in adherence which was maximal at 4 h and 10(-10)M (17.6 +/- 1.4% vs. 6.1 +/- 0.4%, p < 0.01). Conversely, neurotensin stimulation of neutrophils induced a concentration-dependent and rapid ( < 5 min) increase in adherence which was also maximal at 10(-10)M (27.1 +/- 1.9% vs. 10.1 +/- 1.4%, p < 0.01). The effects were reproduced by the carboxy portion of the molecule. Anti-CD11a, -CD11b or -ICAM-1 antibodies significantly decreased the neurotensin-induced increase in adherence. These data suggest an important role for neurotensin in modulating airway inflammation.
Robbins
R A
RA
Research Service, Omaha VA Medical Center, NE, USA.
Nelson
K J
KJ
Gossman
G L
GL
Rubinstein
I
I
D000818:Animals; D001980:Bronchi / Q000166:cytology*; D018845:CD11 Antigens / Q000502:physiology; D002417:Cattle; D002448:Cell Adhesion / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004847:Epithelial Cells; D018799:Intercellular Adhesion Molecule-1 / Q000502:physiology; D009496:Neurotensin / Q000494:pharmacology*; D009504:Neutrophils / Q000187:drug effects* / Q000502:physiology
D018845:CD11 Antigens; D018799:Intercellular Adhesion Molecule-1; D009496:Neurotensin