Notochord signals control the transcriptional cascade of myogenic bHLH genes in somites of quail embryos.
Microsurgical, tissue grafting and in situ hybridization techniques have been used to investigate the role of the neural tube and notochord in the control of the myogenic bHLH genes, QmyoD, Qmyf5, Qmyogenin and the cardiac alpha-actin gene, during somite formation in stage 12 quail embryos. Our results reveal that signals from the axial neural tube/notochord complex control both the activation and the maintenance of expression of QmyoD and Qmyf5 in myotomal progenitor cells during the period immediately following somite formation and prior to myotome differentiation. QmyoD and Qmyf5 expression becomes independent of axial signals during myotome differentiation when somites activate expression of Qmyogenin and alpha-actin. Ablation studies reveal that the notochord controls QmyoD activation and the initiation of the transcriptional cascade of myogenic bHLH genes as epithelial somites condense from segmental plate mesoderm. The dorsal medial neural tube then contributes to the maintenance of myogenic bHLH gene expression in newly formed somites. Notochord grafts can activate ectopic QmyoD expression during somite formation, establishing that the notochord is a necessary and sufficient source of diffusible signals to initiate QmyoD expression. Myogenic bHLH gene expression is localized to dorsal medial cells of the somite by inhibitory signals produced by the lateral plate and ventral neural tube. Signaling models for the activation and maintenance of myogenic gene expression and the determination of myotomal muscle in somites are discussed.
Pownall
M E
ME
Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia 19104-6058, USA.
Strunk
K E
KE
Emerson
C P
CP
D000818:Animals; D017690:Cell Transplantation; D004268:DNA-Binding Proteins; D004627:Embryonic Induction; D018257:Helix-Loop-Helix Motifs; D017403:In Situ Hybridization; D008954:Models, Biological; D009124:Muscle Proteins / Q000096:biosynthesis / Q000235:genetics; D009132:Muscles / Q000196:embryology*; D017570:MyoD Protein / Q000096:biosynthesis / Q000235:genetics; D051787:Myogenic Regulatory Factor 5; D018006:Myogenic Regulatory Factors / Q000096:biosynthesis* / Q000235:genetics; D018008:Myogenin / Q000096:biosynthesis / Q000235:genetics; D009672:Notochord / Q000196:embryology*; D011784:Quail; D015347:RNA Probes; D015534:Trans-Activators; D014158:Transcription, Genetic
D004268:DNA-Binding Proteins; D009124:Muscle Proteins; D017570:MyoD Protein; D051787:Myogenic Regulatory Factor 5; D018006:Myogenic Regulatory Factors; D018008:Myogenin; D015347:RNA Probes; D015534:Trans-Activators