Purification and self-association equilibria of the lysis-lysogeny switch proteins of coliphage 186.
The CI repressor protein, responsible for maintenance of the lysogenic state, and the Apl protein, required for efficient prophage induction, are the two control proteins of the lysis-lysogeny transcriptional switch of coliphage 186.  These proteins have been overexpressed, purified, and their self-association behavior examined by sedimentation equilibrium.  Phage 186 CI dimers self-associate in solution through tetramers to octamers in a concerted process.  The Apl protein of 186 is an unusual example of a helix-turn-helix protein which is monomeric in solution.
Shearwin
K E
KE
Department of Biochemistry, University of Adelaide, Australia.
Egan
J B
JB
D001483:Base Sequence; D001665:Binding Sites; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D003001:Cloning, Molecular; D003090:Coliphages / Q000502:physiology*; D004247:DNA / Q000378:metabolism; D017931:DNA Primers; D004268:DNA-Binding Proteins; D004926:Escherichia coli; D019077:Helix-Turn-Helix Motifs; D007700:Kinetics; D008242:Lysogeny; D046911:Macromolecular Substances; D008961:Models, Structural; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012097:Repressor Proteins / Q000096:biosynthesis / Q000302:isolation & purification* / Q000378:metabolism*; D013816:Thermodynamics; D014158:Transcription, Genetic; D014461:Ultracentrifugation; D014764:Viral Proteins; D054334:Viral Regulatory and Accessory Proteins
D017931:DNA Primers; D004268:DNA-Binding Proteins; D046911:Macromolecular Substances; D012097:Repressor Proteins; D014764:Viral Proteins; D054334:Viral Regulatory and Accessory Proteins; C016649:phage repressor proteins; D004247:DNA