The deduced primary structure of subunit I from cytochrome c oxidase suggests that the genus Polytomella shares a common mitochondrial origin with Chlamydomonas.
We cloned and sequenced the mitochondrial gene encoding subunit I of cytochrome c oxidase (coxI) of Polytomella spp., a colorless alga related to Chlamydomonas. The purpose was to explore whether homology between the two species also exists at the level of a mitochondrial enzyme. The gene is 1512 bp long and contains no introns. The translated protein sequence exhibits 73.8% identity with its Chlamydomonas reinhardtii counterpart. The data obtained support the hypothesis that the separation of the colorless alga from the Chlamydomonas lineage was a late event in evolution, that occurred after the endosymbiotic process that gave rise to mitochondria.
Antaramian
A
A
Departamento de Bioenergética, Instituto de Fisiología Celular, U.N.A.M., Mexico City 045100, Mexico.
Coria
R
R
Ramírez
J
J
González-Halphen
D
D
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002696:Chlamydomonas / Q000145:classification / Q000201:enzymology* / Q000235:genetics; D000460:Chlorophyta / Q000145:classification / Q000201:enzymology* / Q000235:genetics; D003062:Codon; D004272:DNA, Mitochondrial / Q000235:genetics*; D003576:Electron Transport Complex IV / Q000737:chemistry* / Q000235:genetics; D046911:Macromolecular Substances; D008928:Mitochondria / Q000201:enzymology; D008969:Molecular Sequence Data; D010802:Phylogeny; D017386:Sequence Homology, Amino Acid
D003062:Codon; D004272:DNA, Mitochondrial; D046911:Macromolecular Substances; D003576:Electron Transport Complex IV