Isolation and characterization of a novel gene expressed in multiple cancers.
Using differential display method, we have isolated and characterized a novel gene, N8, encoding an approximately 24 kDa protein. It is located on human chromosome 8q13 region. N8 gene is expressed at high levels in tumor derived cell lines from multiple cancers. It is also expressed at higher levels in lung tumors than normal lung tissue. N8 is also differentially expressed in fetal and adult tissues. In adult, N8 is expressed at high levels in brain, kidney, prostate, pancreas and intestine and at very low levels in lung, liver, hematopoietic cells and gonads. During murine embryonic development N8 is expressed in the epithelium of the intestine, stomach, olfactory epithelium, neuronal layers of retina, kidney and salivary gland. Taken together, these results suggest that N8 may play different roles during embryogenesis and in the adult animals.
Chen
S L
SL
Laboratory of Molecular Oncology, National Cancer Institute, Frederick, Maryland 21702-1201, USA.
Maroulakou
I G
IG
Green
J E
JE
Romano-Spica
V
V
Modi
W
W
Lautenberger
J
J
Bhat
N K
NK
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D002460:Cell Line; D002874:Chromosome Mapping; D002898:Chromosomes, Human, Pair 8; D016384:Consensus Sequence; D017931:DNA Primers; D018076:DNA, Complementary; D005260:Female; D015870:Gene Expression; D006801:Humans; D017403:In Situ Hybridization; D007621:Karyotyping; D016350:Leucine Zippers; D008168:Lung / Q000378:metabolism; D008175:Lung Neoplasms / Q000235:genetics; D008297:Male; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000235:genetics*; D009369:Neoplasms / Q000235:genetics* / Q000473:pathology; D009928:Organ Specificity; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D012097:Repressor Proteins / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D014407:Tumor Cells, Cultured
D017931:DNA Primers; D018076:DNA, Complementary; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D012097:Repressor Proteins; C114751:TPD52 protein, human