title: Synonymous codon selection controls in vivo turnover and amount of mRNA in Escherichia coli bla and ompA genes.
abstract: A number of silent codon changes were made in two Escherichia coli genes. For the ompA gene, the replacement of seven consecutive frequently used codons with synonymous infrequently used codons reduced the ompA mRNA level and its half-life. For the bla gene, the exchange of 24 codons for the most frequently used synonymous codons extended the bla mRNA half-life. A modification of ribosome traffic could account for these observations.
authors.affiliation: Laboratoire Structure et Dynamique du Génome, Centre de Génétique Moléculaire, Centre National de la Recherch Scientifique, Gif sur Yvette, France.
authors.forename: A
authors.initials: A
authors.lastname: Deana
authors.forename: R
authors.initials: R
authors.lastname: Ehrlich
authors.forename: C
authors.initials: C
authors.lastname: Reiss
mesh_terms: D000595:Amino Acid Sequence; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D001483:Base Sequence; D003062:Codon / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D006207:Half-Life; D008969:Molecular Sequence Data; D009154:Mutation; D012329:RNA, Bacterial / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D014158:Transcription, Genetic / Q000235:genetics; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D001425:Bacterial Outer Membrane Proteins; D003062:Codon; D012329:RNA, Bacterial; D012333:RNA, Messenger; D001618:beta-Lactamases