title: An on-line semi-automated solid-phase extraction procedure for high-performance liquid chromatographic determination of lonidamine in serum.
abstract: A semi-automated solid-phase extraction procedure on-line with gradient elution reversed-phase chromatography permits the determination of lonidamine and its metabolite in human serum. The average recovery from serum at the 2.5 micrograms ml-1 level was (92.8 +/- 3.4)%. The limit of quantitation for a 100 microliter sample size was 50 ng ml-1. The within-day (n = 5) and between-day (n = 5) relative standard deviations for lonidamine determination in serum samples spiked at the 2.5 micrograms ml-1 level were 2.7% and 4.5%, respectively.
authors.affiliation: Istituto Oncologico, Bari, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Bottalico
authors.forename: G
authors.initials: G
authors.lastname: Micelli
authors.forename: A
authors.initials: A
authors.lastname: Guerrieri
authors.forename: F
authors.initials: F
authors.lastname: Palmisano
authors.forename: V
authors.initials: V
authors.lastname: Lorusso
authors.forename: M
authors.initials: M
authors.lastname: de Lena
mesh_terms: D000970:Antineoplastic Agents / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006801:Humans; D007191:Indazoles / Q000097:blood*; D010051:Ovarian Neoplasms / Q000097:blood / Q000188:drug therapy
chemical_list: D000970:Antineoplastic Agents; D007191:Indazoles; C016371:lonidamine