title: 3pK, a new mitogen-activated protein kinase-activated protein kinase located in the small cell lung cancer tumor suppressor gene region.
abstract: NotI linking clones, localized to the human chromosome 3p21.3 region and homozygously deleted in small cell lung cancer cell lines NCI-H740 and NCI-H1450, were used to search for a putative tumor suppressor gene(s). One of these clones, NL1G210, detected a 2.5-kb mRNA in all examined human tissues, expression being especially high in the heart and skeletal muscle. Two overlapping cDNA clones containing the entire open reading frame were isolated from a human heart cDNA library and fully characterized. Computer analysis and a search of the GenBank database to reveal high sequence identity of the product of this gene to serine-threonine kinases, especially to mitogen-activated protein kinase-activated protein kinase 2, a recently described substrate of mitogen-activated kinases. Sequence identitiy was 72% at the nucleotide level and 75% at the amino acid level, strongly suggesting that this protein is a serine-threonine kinase. Here we demonstrate that the new gene, referred to as 3pK (for chromosome 3p kinase), in fact encodes a mitogen-activated protein kinase-regulated protein serine-threonine kinase with a novel substrate specificity.
authors.affiliation: Biological Carcinogenesis and Development Program, PRI/DynCorp, National Cancer Institute, Frederick Cancer Research and Development Center, Maryland 21702-1201, USA.
authors.forename: G
authors.initials: G
authors.lastname: Sithanandam
authors.forename: F
authors.initials: F
authors.lastname: Latif
authors.forename: F M
authors.initials: FM
authors.lastname: Duh
authors.forename: R
authors.initials: R
authors.lastname: Bernal
authors.forename: U
authors.initials: U
authors.lastname: Smola
authors.forename: H
authors.initials: H
authors.lastname: Li
authors.forename: I
authors.initials: I
authors.lastname: Kuzmin
authors.forename: V
authors.initials: V
authors.lastname: Wixler
authors.forename: L
authors.initials: L
authors.lastname: Geil
authors.forename: S
authors.initials: S
authors.lastname: Shrestha
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D018288:Carcinoma, Small Cell / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification; D016147:Genes, Tumor Suppressor; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D008175:Lung Neoplasms / Q000235:genetics*; D008969:Molecular Sequence Data; D017346:Protein Serine-Threonine Kinases / Q000235:genetics* / Q000302:isolation & purification; D016415:Sequence Alignment
chemical_list: D018076:DNA, Complementary; D047908:Intracellular Signaling Peptides and Proteins; C098416:MAP-kinase-activated kinase 3; D017346:Protein Serine-Threonine Kinases