title: Expression of RHD and RHCE gene products using retroviral transduction of K562 cells establishes the molecular basis of Rh blood group antigens.
abstract: Retroviral-mediated gene transfer using cDNA transcripts of the RHD and RHCE genes resulted in the isolation of K562 clones expressing D and G or c and E antigens, respectively. These results represent the first direct demonstration that the RHD gene encodes the D and G antigens and the RHCE gene encodes the c and E antigens. Both c and E antigens were expressed after transduction of K562 cells with a single cDNA, indicating that the c antigen does not arise by alternative splicing (exon skipping) of the product of the RHCE gene, as has been suggested.
authors.affiliation: International Blood Group Reference Laboratory, Bristol, UK.
authors.forename: J S
authors.initials: JS
authors.lastname: Smythe
authors.forename: N D
authors.initials: ND
authors.lastname: Avent
authors.forename: P A
authors.initials: PA
authors.lastname: Judson
authors.forename: S F
authors.initials: SF
authors.lastname: Parsons
authors.forename: P G
authors.initials: PG
authors.lastname: Martin
authors.forename: D J
authors.initials: DJ
authors.lastname: Anstee
mesh_terms: D000941:Antigens / Q000235:genetics* / Q000276:immunology; D001483:Base Sequence; D001789:Blood Group Antigens / Q000235:genetics* / Q000276:immunology; D003001:Cloning, Molecular; D005434:Flow Cytometry; D018014:Gene Transfer Techniques; D006801:Humans; D008969:Molecular Sequence Data; D014407:Tumor Cells, Cultured
chemical_list: D000941:Antigens; D001789:Blood Group Antigens