title: Isolation and characterization of a novel Deinococcus radiodurans mutant abnormally susceptible to mutation induction by UV, gamma-ray, and mitomycin C.
abstract: We isolated and characterized a novel, radiation 'hypermutable' mutant of Deinococcus radiodurans. Compared with the wild-type strain D. radiodurans IR, this mutator strain, designated S101, exhibited sensitivity to UV light, gamma-ray, mitomycin C, and N-methyl-N'-nitro-N-nitrosoguanidine. Spontaneous revertants of S101 that restored wild-type phenotype (non-mutability and resistance to these DNA-damaging agents) were also isolated. Furthermore, the increased susceptibility to DNA-damaging agents and mutability observed in S101 could be mimicked by treating D. radiodurans IR with Mn(II) ions. Our results suggest a putative new pathway of DNA repair in the extremely radioresistant bacterium D. radiodurans.
authors.affiliation: Institute for Radiation Biology, National Tsing Hua University, Hsinschu, Taiwan, Republic of China.
authors.forename: C L
authors.initials: CL
authors.lastname: Lin
authors.forename: S T
authors.initials: ST
authors.lastname: Tan
mesh_terms: D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D004249:DNA Damage; D004260:DNA Repair; D005720:Gamma Rays; D008837:Micrococcus / Q000187:drug effects / Q000235:genetics / Q000528:radiation effects*; D016685:Mitomycin / Q000494:pharmacology*; D009154:Mutation; D011836:Radiation Tolerance; D014466:Ultraviolet Rays
chemical_list: D000903:Antibiotics, Antineoplastic; D016685:Mitomycin