title: Activation of factor VII during alimentary lipemia occurs in healthy adults and patients with congenital factor XII or factor XI deficiency, but not in patients with factor IX deficiency.
abstract: Factor VII activity (FVIIc), a risk marker for coronary heart disease, is increased during postprandial lipemia. Factor VII activation accompanies lipolysis of triglyceride-rich lipoproteins, but the nature of this association and whether it is causal remain uncertain. To explore this issue, four patients with homozygous factor XII deficiency, four with complete factor XI deficiency, six with factor IX deficiency, and their respective age- and sex-matched controls were given two isocaloric dietary regimens, one providing on average 136 g fat and the other 19 g fat. Blood was taken before breakfast, immediately before lunch at 195 minutes, and at completion of the study at 390 minutes. All samples for each subject and matched control were assayed as one batch for FVIIc, activated factor VII, and factor VII antigen (FVIIag). Activation of factor VII was observed with the high-fat regimen but not with the low-fat regimen in all controls, factor XII-deficient patients, and factor XI-deficient patients. No factor VII activation was observed during either regimen in factor IX-deficient patients, but a normal postprandial responsiveness of factor VII to dietary fat was restored in one patient who replicated the study after factor IX therapy. Plasma FVIIag was not altered postprandially in either regimen in any group of patients or controls. Factor IX apparently plays an obligatory role in the postprandial activation of factor VII, although the mechanism remains to be determined.
authors.affiliation: Medical Research Council Epidemiology and Medical Care Unit, Medical College of St Bartholomew's Hospital, London.
authors.forename: G J
authors.initials: GJ
authors.lastname: Miller
authors.forename: J C
authors.initials: JC
authors.lastname: Martin
authors.forename: K A
authors.initials: KA
authors.lastname: Mitropoulos
authors.forename: M P
authors.initials: MP
authors.lastname: Esnouf
authors.forename: J A
authors.initials: JA
authors.lastname: Cooper
authors.forename: J H
authors.initials: JH
authors.lastname: Morrissey
authors.forename: D J
authors.initials: DJ
authors.lastname: Howarth
authors.forename: E G
authors.initials: EG
authors.lastname: Tuddenham
mesh_terms: D000328:Adult; D015992:Body Mass Index; D002784:Cholesterol / Q000097:blood; D018752:Diet, Fat-Restricted; D004041:Dietary Fats / Q000008:administration & dosage / Q000494:pharmacology*; D004435:Eating; D004789:Enzyme Activation / Q000187:drug effects; D005164:Factor IX / Q000494:pharmacology; D005167:Factor VII / Q000378:metabolism*; D015942:Factor VIIa / Q000096:biosynthesis*; D005173:Factor XI Deficiency / Q000097:blood*; D005175:Factor XII Deficiency / Q000097:blood*; D005215:Fasting; D005260:Female; D002836:Hemophilia B / Q000097:blood*; D006801:Humans; D008066:Lipolysis; D008297:Male; D008875:Middle Aged; D012307:Risk Factors; D014280:Triglycerides / Q000097:blood
chemical_list: D004041:Dietary Fats; D014280:Triglycerides; D005167:Factor VII; D005164:Factor IX; D002784:Cholesterol; D015942:Factor VIIa