Immunohistochemical study of the existence and coexistence of catecholamine synthesizing enzymes and some neuropeptides in perivascular nerve fibres of the main thoraco-cranial arteries in the pig.
The presence and pattern of coexistence of catecholamine-synthesizing enzymes and some neuropeptides in nerve fibres supplying thoraco-cranial arteries of the sexually immature gilts were investigated in whole mount preparations. The studied substances included: tyrosine hydroxylase (TH), dopamine-beta-hydroxylase (D beta H) (as markers of catecholaminergic nerve fibres), neuropeptide Y (NPY), Leu5-enkephalin (LENK), vasoactive intestinal polipeptide (VIP), calcitonin gene-related peptide (CGRP), substance P (SP), galanin (GAL), somatostatin (SOM) and serotonin (5-HT). The arteries were found to be richly supplied by TH/D beta H-immunoreactive (TH/D beta H) nerve fibers. Of the neuropeptides studied, NPY (rich innervation), LENK (moderate innervation), VIP (moderate innervation) and CGRP, SP, GAL (only a few nerve fibres) were detected in periarterial nerves. The following patterns of coexistence of the studied substances were found: TH+/D beta H+, TH+/D beta H+/NPY+, TH+/D beta H+/LENK+, TH-/D beta H-/NPY+, TH-/D beta H-/VIP+, TH+/D beta H+/VIP+ (only a few nerve fibres in the cerebral arteries), LENK+/NPY+, LENK-/NPY+, LENK+/NPY-. No SOM and 5-HT-positive structures were observed in the porcine blood vessels.
Sienkiewicz
W
W
Department of Animal Anatomy, Faculty of Veterinary Medicine, Agricultural and Technical University, Olsztyn, Poland.
Majewski
M
M
Kaleczyc
J
J
Lakomy
M
M
D000818:Animals; D001011:Aorta / Q000201:enzymology / Q000294:innervation; D001158:Arteries / Q000201:enzymology / Q000294:innervation*; D015415:Biomarkers / Q000032:analysis; D015740:Calcitonin Gene-Related Peptide / Q000032:analysis; D002339:Carotid Arteries / Q000201:enzymology / Q000294:innervation; D002395:Catecholamines / Q000096:biosynthesis; D002536:Cerebral Arteries / Q000201:enzymology / Q000294:innervation; D004299:Dopamine beta-Hydroxylase / Q000032:analysis / Q000276:immunology / Q000378:metabolism*; D004743:Enkephalin, Leucine / Q000032:analysis; D019004:Galanin / Q000032:analysis; D007150:Immunohistochemistry; D009412:Nerve Fibers / Q000737:chemistry* / Q000201:enzymology; D009478:Neuropeptide Y / Q000032:analysis; D009479:Neuropeptides / Q000032:analysis*; D012701:Serotonin / Q000032:analysis; D013004:Somatostatin / Q000032:analysis; D013373:Substance P / Q000032:analysis; D013552:Swine; D014446:Tyrosine 3-Monooxygenase / Q000032:analysis / Q000276:immunology / Q000378:metabolism*; D014660:Vasoactive Intestinal Peptide / Q000032:analysis
D015415:Biomarkers; D002395:Catecholamines; D009478:Neuropeptide Y; D009479:Neuropeptides; D012701:Serotonin; D013373:Substance P; D014660:Vasoactive Intestinal Peptide; D013004:Somatostatin; D004743:Enkephalin, Leucine; D019004:Galanin; D014446:Tyrosine 3-Monooxygenase; D004299:Dopamine beta-Hydroxylase; D015740:Calcitonin Gene-Related Peptide