Nucleotide sequence and characterization of erythromycin resistance determinant that encodes macrolide 2'-phosphotransferase I in Escherichia coli.
The DNA fragment (3.3 kb) containing the erythromycin resistance determinant was cloned from Escherichia coli Tf481A and sequenced. Deletion and complementation analyses indicated that the expression of high-level resistance to erythromycin requires two genes, mphA and mrx, which encode macrolide 2'-phosphotransferase I and an unidentified hydrophobic protein, respectively.
Noguchi
N
N
Department of Microbiology, School of Pharmacy, Tokyo University of Pharmacy and Life Science, Japan.
Emura
A
A
Matsuyama
H
H
O'Hara
K
K
Sasatsu
M
M
Kono
M
M
D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000378:metabolism* / Q000494:pharmacology*; D001483:Base Sequence; D004352:Drug Resistance, Microbial / Q000235:genetics*; D004917:Erythromycin / Q000494:pharmacology*; D004926:Escherichia coli / Q000201:enzymology*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010770:Phosphotransferases / Q000235:genetics*; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics*
D000900:Anti-Bacterial Agents; D004917:Erythromycin; D010770:Phosphotransferases; D017853:Phosphotransferases (Alcohol Group Acceptor); C061369:macrolide 2'-kinase