title: Calreticulin binding affinity for glycosylated laminin.
abstract: Several lines of evidence indicate that calreticulin has lectin-like properties. As a molecular chaperone, calreticulin binds preferentially to nascent glycoproteins via their immature carbohydrates; this property closely resembles that seen for calnexin, a chaperone with extensive molecular identity to calreticulin. A cell surface form of calreticulin also exhibits lectin-like properties, binding specific oligomannosides including those covalently linked to laminin. In the present study we examined the interaction between calreticulin and laminin by means of surface plasmon resonance. The results show that calreticulin specifically binds to glycosylated laminin but fails to specifically bind tunicamycin-derived unglycosylated laminin or bovine serum albumin. Calreticulin binding to glycosylated laminin requires calcium and is abolished in the presence of EDTA. Scatchard analysis of binding yields an apparent association constant, Ka, of 2.1 +/- 0.9 x 10(6) m-1 while kinetic analysis yields an estimate of the association on rate, (Kassoc), as 2 x 10(5) m-1 s-1. The composite results support calreticulin's lectin-like properties as well as its proposed role in laminin recognition, both in the cell interior and on the cell surface.
authors.affiliation: Randall Institute, King's College, London WC2B 5RL, United Kingdom.
authors.forename: J M
authors.initials: JM
authors.lastname: McDonnell
authors.forename: G E
authors.initials: GE
authors.lastname: Jones
authors.forename: T K
authors.initials: TK
authors.lastname: White
authors.forename: M L
authors.initials: ML
authors.lastname: Tanzer
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D002135:Calcium-Binding Proteins / Q000378:metabolism*; D037282:Calreticulin; D002417:Cattle; D006023:Glycoproteins / Q000378:metabolism; D007700:Kinetics; D007797:Laminin / Q000378:metabolism*; D037102:Lectins; D051379:Mice; D011485:Protein Binding; D012261:Ribonucleoproteins / Q000378:metabolism*
chemical_list: D002135:Calcium-Binding Proteins; D037282:Calreticulin; D006023:Glycoproteins; D007797:Laminin; D037102:Lectins; D012261:Ribonucleoproteins; D002118:Calcium