title: Deletion of glycoprotein gE reduces the propagation of pseudorabies virus in the nervous system of mice after intranasal inoculation.
abstract: A pseudorabies virus (PrV) mutant, deficient in the nonessential glycoprotein E (gE) and expressing the LacZ gene (gE- beta gal+ PrV), and its rescued virus were inoculated intranasally in mice. The median lethal dose of gE- beta gal+ PrV was similar to that of the parental Kaplan strain, but mice survived longer and did not develop symptoms of pseudorabies. In the nasal mucosa, gE- beta gal+ PrV replicated less efficiently than rescued virus. gE- beta gal+ PrV could infect first-order trigeminal and sympathetic neurons innervating the nasal mucosa. However, transneuronal transfer to second-order cells groups did not occur in trigeminal pathways and was severely reduced in sympathetic pathways. The mutant was also unable to propagate in the parasympathetic system. In contrast, gE-rescued virus was transferred transneuronally in trigeminal, sympathetic, and parasympathetic pathways, like wild-type PrV. These findings provide further evidence that deletion of gE specifically affects transneuronal transfer of PrV more than penetration and multiplication of the virus in first-order neurons.
authors.affiliation: Laboratoire de Génétique des Virus, C.N.R.S., Gif-sur-Yvette, France.
authors.forename: N
authors.initials: N
authors.lastname: Babic
authors.forename: B
authors.initials: B
authors.lastname: Klupp
authors.forename: A
authors.initials: A
authors.lastname: Brack
authors.forename: T C
authors.initials: TC
authors.lastname: Mettenleiter
authors.forename: G
authors.initials: G
authors.lastname: Ugolini
authors.forename: A
authors.initials: A
authors.lastname: Flamand
mesh_terms: D000281:Administration, Intranasal; D000818:Animals; D002522:Chlorocebus aethiops; D004195:Disease Models, Animal; D005260:Female; D017353:Gene Deletion; D011558:Herpesvirus 1, Suid / Q000235:genetics / Q000502:physiology*; D051379:Mice; D009296:Nasal Cavity / Q000821:virology; D009474:Neurons / Q000821:virology*; D009833:Olfactory Pathways / Q000166:cytology / Q000821:virology; D010275:Parasympathetic Nervous System / Q000821:virology; D011557:Pseudorabies / Q000821:virology*; D012137:Respiratory System / Q000166:cytology / Q000821:virology; D013564:Sympathetic Nervous System / Q000821:virology; D014276:Trigeminal Nerve / Q000821:virology; D014709:Vero Cells; D014759:Viral Envelope Proteins / Q000235:genetics / Q000502:physiology*; D014779:Virus Replication
chemical_list: D014759:Viral Envelope Proteins; C089580:glycoprotein E, Suid herpesvirus 1