Effects of GGF/neuregulins on neuronal survival and neurite outgrowth correlate with erbB2/neu expression in developing rat retina.
We have examined the expression of neuregulin and its putative receptors, erbB2/neu, erbB3 and erbB4/tyro2 during retinal development, and tested several potential functions of this class of molecules in dissociated rat retinal cell cultures. At least one form of neuregulin is expressed in the retina, from the earliest stages of retinal development examined; in addition, all three of the known receptors are expressed by retinal neurons in a developmentally regulated manner. When added to cultures of embryonic or neonatal rat retinal cells, neuregulin (rhGGF2) promotes survival and neurite extension from retinal neurons in a dose-dependent manner. These results indicate that in addition to their well described effects on glia, the neuregulins also have direct effects on central nervous system neurons.
Bermingham-McDonogh
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Cambridge NeuroScience, Seattle, WA 98195, USA.
McCabe
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D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D016895:Culture Media, Serum-Free; D066246:ErbB Receptors / Q000096:biosynthesis*; D018815:Genes, erbB; D006023:Glycoproteins / Q000494:pharmacology*; D017403:In Situ Hybridization; D009414:Nerve Growth Factors / Q000494:pharmacology*; D020880:Neuregulins; D016501:Neurites / Q000187:drug effects*; D009924:Organ Culture Techniques; D012333:RNA, Messenger / Q000302:isolation & purification; D051381:Rats; D011919:Rats, Inbred Strains; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000096:biosynthesis*; D012160:Retina / Q000187:drug effects / Q000196:embryology* / Q000254:growth & development; D012165:Retinal Ganglion Cells / Q000187:drug effects
D016895:Culture Media, Serum-Free; D066246:ErbB Receptors; D006023:Glycoproteins; D009414:Nerve Growth Factors; D020880:Neuregulins; D012333:RNA, Messenger; D018719:Erb-b2 Receptor Tyrosine Kinases; C064740:epidermal growth factor receptor-neu receptor