Fine mapping of the human endothelin-converting enzyme gene by fluorescent in situ hybridization and radiation hybrids.
Based on the sequence of the human endothelin-1-converting enzyme (hECE-1) cDNA, we cloned a 1982 bp cDNA fragment and we amplified by PCR a 3' fragment located on the last exon (exon 19). Human metaphase chromosomes were studied by Fluorescent in Situ Hybridization (FISH) using the 1982 digoxigenated hECE-1 fragment as a probe and chromosome 1-specific probes. Twin spot signals on each of the two homologous chromosomes 1 were found by FISH on band p36. The results of monochromosomal hybrids confirmed that hECE-1 is on chromosome 1. Radiation hybrid mapping localized the hECE-1 gene 3.15 cR far from D1S2436 (WI-3177), at about 25 cM from the telomere of the short arm, possibly at the border between 1p36.3 and 1p36.2.
Albertin
G
G
Department of Clinical Medicine, University of Padova Medical School, University Hospital, Italy.
Rossi
G P
GP
Majone
F
F
Tiso
N
N
Mattara
A
A
Danieli
G A
GA
Pessina
A C
AC
Palù
G
G
D000818:Animals; D016282:Aspartic Acid Endopeptidases / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D002874:Chromosome Mapping; D002878:Chromosomes, Human, Pair 1; D018076:DNA, Complementary; D000072396:Endothelin-Converting Enzymes; D006801:Humans; D006822:Hybrid Cells / Q000528:radiation effects*; D017403:In Situ Hybridization; D008666:Metalloendopeptidases / Q000378:metabolism; D008969:Molecular Sequence Data; D012377:Rodentia
D018076:DNA, Complementary; D016282:Aspartic Acid Endopeptidases; D008666:Metalloendopeptidases; D000072396:Endothelin-Converting Enzymes