Topography and immunogenicity of bluetongue virus VP7 epitopes.
The core of bluetongue virus (BTV) consists of ten dsRNA viral genome segments and five proteins, including two major (VP7 and VP3) and three minor (VP1, VP4 and VP6) components. The major core protein VP7 is believed to be an important structural constituent because it interacts, not only with the underlying core protein VP3, but also with two outer capsid proteins (VP2 and VP5). In this communication we summarise data on the mapping of at least six different epitopes of VP7 distributed along the molecule. Two of the six epitopes have not been mapped previously. The accessibility of these epitopes in intact virions and core particles was analysed using immunoelectron microscopy. The epitope located near the N-terminus of VP7 was accessible at the surface of intact virions and core particles. Epitopes in other parts of the VP7 molecule were detected weakly in core particles but not in intact virions. These results support the proposal that VP7 molecules are orientated with their N-terminus accessible on the surface of either the particle or at least one of the three different channels observed by cryoelectron microscopy in the outer capsid layer. Analysis of the immune response to BTV-infected or -immunised sheep and rabbits to three selected epitopes, which are located in different regions of the VP7 molecule, demonstrated that all of them were recognised by the animals tested. These results provided further molecular evidence suggesting that VP7 is indeed a major immunogenic antigen ideal for BTV antibody detection.
Wang
L F
LF
CSIRO Australian Animal Health Laboratory, Geelong, Victoria, Australia.
Hyatt
A D
AD
Whiteley
P L
PL
Andrew
M
M
Li
J K
JK
Eaton
B T
BT
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis / Q000097:blood; D000956:Antigens, Viral / Q000032:analysis / Q000235:genetics / Q000276:immunology*; D001483:Base Sequence; D001819:Bluetongue / Q000175:diagnosis / Q000276:immunology; D001820:Bluetongue virus / Q000235:genetics* / Q000276:immunology* / Q000648:ultrastructure; D002213:Capsid / Q000032:analysis / Q000235:genetics / Q000276:immunology*; D036022:Capsid Proteins; D002417:Cattle; D002460:Cell Line; D017931:DNA Primers; D004279:DNA, Viral / Q000235:genetics; D000939:Epitopes / Q000032:analysis / Q000737:chemistry; D016679:Genome, Viral; D016253:Microscopy, Immunoelectron; D008969:Molecular Sequence Data; D016296:Mutagenesis; D016133:Polymerase Chain Reaction; D011487:Protein Conformation; D012330:RNA, Double-Stranded / Q000235:genetics; D011817:Rabbits; D011993:Recombinant Fusion Proteins / Q000032:analysis / Q000096:biosynthesis / Q000276:immunology; D017384:Sequence Deletion; D012756:Sheep
D000914:Antibodies, Viral; D000956:Antigens, Viral; D036022:Capsid Proteins; D017931:DNA Primers; D004279:DNA, Viral; D000939:Epitopes; D012330:RNA, Double-Stranded; D011993:Recombinant Fusion Proteins; C050779:VP7 protein, Rotavirus