[Use of LKhM-80 chromatograph in the analysis of urinary steroids].
The paper describes a simple way of updating a Russian production-type LKaM-80 chromatograph in order to make a gas chromatographic analysis of urinary steroids for separation by using high performance capillary columns. To connect capillary columns, the units consisting of a gas heaving T-joint, a stream separating chamber, and a releasing restrictor were applied. To achieve satisfactory results, it is necessary to place a column through the tube connecting the union for attachment of the column and the flame-ionization detector so that the end of a capillary should be located at the detector as soon as closer. The updated chromatograph has been successfully used to obtain urinary steroidal profiles which are essential for the differential diagnosis of causes of hyperandrogyny in females, including pregnants.
Orlov
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EN
Antipov
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EM
D000728:Androgens / Q000652:urine; D002849:Chromatography, Gas / Q000295:instrumentation*; D005260:Female; D006801:Humans; D008297:Male; D011247:Pregnancy; D013256:Steroids / Q000652:urine*
D000728:Androgens; D013256:Steroids