Repair mechanisms of UV-induced DNA damage in soybean chloroplasts.
In order to better understand the biochemical mechanisms of DNA metabolism in chloroplasts, repair of UV induced plastome damage in vivo was determined by exposure of soybean suspension cells to UV light and subsequent quantitation of the damage remaining in nuclear and chloroplast encoded genes with time by quantitative polymerase chain reaction (QPCR). The kinetics of damage repair in the nuclear rbcS gene suggest that photoreactivation and dark mechanisms are active, while for the plastome encoded psbA gene only a light-dependent repair process was detected which is considerably slower than would be expected for photolyase-mediated photoreactivation.
Cannon
G C
GC
Department of Chemistry and Biochemistry, University of Southern Mississippi, Hattiesburg 39406-5043, USA.
Hedrick
L A
LA
Heinhorst
S
S
D001483:Base Sequence; D002467:Cell Nucleus / Q000378:metabolism / Q000528:radiation effects; D002478:Cells, Cultured; D002736:Chloroplasts / Q000378:metabolism / Q000528:radiation effects*; D004249:DNA Damage; D017931:DNA Primers; D004260:DNA Repair; D018744:DNA, Plant / Q000096:biosynthesis / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D017343:Genes, Plant; D007700:Kinetics; D008969:Molecular Sequence Data; D015388:Organelles / Q000378:metabolism; D045322:Photosynthetic Reaction Center Complex Proteins / Q000096:biosynthesis; D045332:Photosystem II Protein Complex; D016133:Polymerase Chain Reaction; D012273:Ribulose-Bisphosphate Carboxylase / Q000096:biosynthesis; D013025:Glycine max / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D014466:Ultraviolet Rays
D017931:DNA Primers; D018744:DNA, Plant; D045322:Photosynthetic Reaction Center Complex Proteins; D045332:Photosystem II Protein Complex; D012273:Ribulose-Bisphosphate Carboxylase