title: Determination of tryptophan and its kynurenine pathway metabolites in human serum by high-performance liquid chromatography with simultaneous ultraviolet and fluorimetric detection.
abstract: An isocratic reversed-phase high-performance liquid chromatographic method for the simultaneous determination of tryptophan and four metabolites of the kynurenine pathway (kynurenine, 3-hydroxykynurenine, kynurenic acid and 3-hydroxyanthranilic acid) in human serum is described. This new method, which uses both isocratic elution and two on-line connected programmable ultraviolet and spectrofluorimetric detectors, allows the determination of these metabolites, in the physiological ranges, with satisfying specificity and sensitivity within 30 min.
authors.affiliation: Service de Biochimie, Hôpital Beaujon 100, Clichy, France.
authors.forename: C
authors.initials: C
authors.lastname: Hervé
authors.forename: P
authors.initials: P
authors.lastname: Beyne
authors.forename: H
authors.initials: H
authors.lastname: Jamault
authors.forename: E
authors.initials: E
authors.lastname: Delacoux
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D007737:Kynurenine / Q000097:blood*; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D014364:Tryptophan / Q000097:blood*
chemical_list: D007737:Kynurenine; D014364:Tryptophan