Human p300 protein is a coactivator for the transcription factor MyoD.
Human p300 protein is a cellular target of adenoviral E1A oncoprotein and a potential transcriptional coactivator. Both p300 and Rb family protein-binding regions of E1A are required for the repression of muscle gene expression, which is regulated by MyoD family transactivators. This implies that p300 is involved in MyoD-dependent transactivation. We show that the repression of MyoD-mediated E box (MyoD consensus) reporter activity by E1A is correlated with its interaction with p300, indicating that p300 participates in MyoD-dependent transactivation. In addition, p300 is able to interact both in vivo and in vitro with MyoD through a portion at the carboxyl-terminal cysteine/histidine-rich domain and associates with the components of the basal transcriptional complex through its two separate transactivation domains at the amino and carboxyl termini. Consistent with its role as a coactivator, p300 potentiates MyoD-activated transcription.
Yuan
W
W
Jefferson Cancer Institute, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA.
Condorelli
G
G
Caruso
M
M
Felsani
A
A
Giordano
A
A
D003545:Cysteine / Q000737:chemistry; D004742:Enhancer Elements, Genetic; D006639:Histidine / Q000737:chemistry; D006801:Humans; D046911:Macromolecular Substances; D017570:MyoD Protein / Q000737:chemistry* / Q000502:physiology; D009687:Nuclear Proteins / Q000737:chemistry*; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D017384:Sequence Deletion; D013329:Structure-Activity Relationship; D015534:Trans-Activators; D014157:Transcription Factors / Q000737:chemistry*; D014158:Transcription, Genetic; D015533:Transcriptional Activation
D046911:Macromolecular Substances; D017570:MyoD Protein; D009687:Nuclear Proteins; D015534:Trans-Activators; D014157:Transcription Factors; D006639:Histidine; D003545:Cysteine