A third human tissue transglutaminase homologue as a result of alternative gene transcripts.
A 2.4 kilobase (kb) cDNA encoding a new form of human tissue transglutaminase homologue (TGH2) was isolated from retinoic acid-induced human erythroleukemia cell (HEL) library. Full-length cDNA analysis gives an open reading frame coding for a polypeptide of 349 amino acid residues with a molecular mass of 38,700 Da. This variant differs from the previously reported homologue TGH in that it is 199 amino acids shorter and has an alternative, 63 amino acid COOH-terminal peptide. The 3'-untranslated region of the cDNA also differs from the previously reported sequences for both TGH and human tissue transglutaminase. The region coding for the first 286 amino acids of TGH2, which contains the active site is identical to TGH. Immunoprecipitation of the in vitro translation product from a synthetic TGH2 mRNA and immunoprecipitation of total protein of human heart, liver, kidney and cultured erythroleukemia HEL cell, revealed a protein with a molecular mass of 37,000 Da by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Comparison of the cDNA sequence for the previously known tissue transglutaminases with genomic DNA and the TGH2 cDNA described here indicate that the sequence divergence points correlate with known intron-exon boundaries. The smaller RNA species encode for truncated proteins with novel carboxyl termini. The TGH cDNA and the TGH2 cDNA both produce transcripts which start with the regular coding sequence for TGase and then fail to splice at specific donor sites, resulting in the use of an alternative exon that contains a stop codon.
Fraij
B M
BM
Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater 74078, USA.
Gonzales
R A
RA
D000595:Amino Acid Sequence; D001483:Base Sequence; D002460:Cell Line; D002474:Cell-Free System; D018076:DNA, Complementary / Q000235:genetics; D005091:Exons / Q000235:genetics; D005347:Fibroblasts / Q000737:chemistry; D005796:Genes; D006801:Humans; D004915:Leukemia, Erythroblastic, Acute / Q000139:chemically induced / Q000235:genetics / Q000473:pathology; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000235:genetics; D009928:Organ Specificity; D010920:Placenta / Q000737:chemistry; D016133:Polymerase Chain Reaction; D014176:Protein Biosynthesis; D012326:RNA Splicing; D012334:RNA, Neoplasm / Q000235:genetics; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic; D011503:Transglutaminases / Q000096:biosynthesis / Q000145:classification / Q000235:genetics*; D014212:Tretinoin / Q000633:toxicity; D014407:Tumor Cells, Cultured
D018076:DNA, Complementary; D009363:Neoplasm Proteins; D012334:RNA, Neoplasm; D014212:Tretinoin; D011503:Transglutaminases