Binding specificity and tissue-specific expression pattern of the Arabidopsis bZIP transcription factor TGA2.
The binding specificity and tissue-specific expression pattern of TGA2 (AHBP-1b), an Arabidopsis bZIP transcription factor have been determined. Filter-binding and gel-shift assays showed that TGA2 has high affinity for C-boxes (ATGACGTCAT). In this respect TGA2 is similar to other members of the Arabidopsis TGA family (such as TGA1, TGA3 and OBF4) and to tobacco TGA1a. Genomic Southern blot analysis confirmed that TGA2 is a member of the gene family. Northern blot analysis showed that the gene is expressed at similar levels in root, stem, leaf and flower t at somewhat lower levels in siliques. TGA3 was also found to be expressed at the same level throughout the plant, whereas genes encoding TGA1 and OBF4 have relatively high RNA expression levels in root. The differential expression of these genes suggests that they have distinct functions.
de Pater
S
S
Center for Phytotechnology, Leiden University, The Netherlands.
Pham
K
K
Memelink
J
J
Kijne
J
J
D017360:Arabidopsis / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D050976:Basic-Leucine Zipper Transcription Factors; D001665:Binding Sites / Q000235:genetics; D015152:Blotting, Northern; D015139:Blotting, Southern; D015342:DNA Probes / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D004926:Escherichia coli / Q000737:chemistry / Q000235:genetics; D051790:G-Box Binding Factors; D018506:Gene Expression Regulation, Plant / Q000235:genetics*; D016350:Leucine Zippers / Q000235:genetics; D008969:Molecular Sequence Data; D010947:Plants, Toxic; D014026:Nicotiana / Q000737:chemistry / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
D050976:Basic-Leucine Zipper Transcription Factors; D015342:DNA Probes; D004268:DNA-Binding Proteins; D051790:G-Box Binding Factors; D014157:Transcription Factors