Relative contribution of the selectins in the neutrophil recruitment caused by the chemokine cytokine-induced neutrophil chemoattractant (CINC).
Rat CINC induced a dose- and time-dependent accumulation of neurophils into murine air-pouches, a response which was inhibited by two selective H1-antagonists, mepyramine and triprolidine (approximately 60%). As pretreatment with fucoidin abolished CINC effect, the relative time-related contribution of selectins on this process was then investigated by using specific monoclonal antibodies (mAb). Anti-CD62L mAb gave a similar degree of inhibition of CINC-induced cell accumulation both at the 2h and 4h time-point (approximately 75%). Anti-CD62P mAb, but not the anti-CD62E mAb, inhibited PMN accumulation at 2h (65%), but only co-administration of these two mAbs inhibited the cell response to CINC at the 4h time-point (90%). Thus endogenous histamine, CD62L, CD62P, and CD62E, though to a different degree, are required for PMN extravasation observed in response to CINC administration.
Harris
J G
JG
Department of Biochemical Pharmacology, William Harvey Research Institute, London, United Kingdom.
Flower
R J
RJ
Watanabe
K
K
Tsurufuji
S
S
Wolitzky
B A
BA
Perretti
M
M
D000818:Animals; D002465:Cell Movement / Q000502:physiology; D054360:Chemokine CXCL1; D019743:Chemokines, CXC; D002630:Chemotactic Factors / Q000502:physiology*; D006133:Growth Substances / Q000502:physiology*; D006632:Histamine / Q000502:physiology; D036341:Intercellular Signaling Peptides and Proteins; D008297:Male; D051379:Mice; D009504:Neutrophils / Q000166:cytology*; D010972:Platelet Activating Factor / Q000502:physiology; D051381:Rats; D019087:Selectins / Q000502:physiology*
D054360:Chemokine CXCL1; D019743:Chemokines, CXC; D002630:Chemotactic Factors; C475895:Cxcl1 protein, mouse; C513297:Cxcl1 protein, rat; D006133:Growth Substances; D036341:Intercellular Signaling Peptides and Proteins; D010972:Platelet Activating Factor; D019087:Selectins; D006632:Histamine