Changes in the gene expression of a protein with the cdc10/SWI6 motif, V-1, during rat follicular development and corpus luteum formation.
We examined the gene expression of V-1, a novel soluble protein with the cdc10/SWI6 motif, in pseudopregnant rat ovaries. Northern blot analysis on days 1, 5, and 11 of pseudopregnancy revealed an approximately 2-fold increase in the V-1 messenger RNA (mRNA) expression level on day 5 to that on day 1, and no significant change was observed between those on day 5 and day 11. An injection of human CG on days 5 further increased th V-1 mRNA level to about 1.6-fold of that of the untreated control. Western blot analysis showed higher V-1 protein expression on days 5 and 11 of pseudopregnancy than that on day 1. In situ hybridization and immunohistochemistry with ovaries on day 3 of pseudopregnancy showed that luteal cells of corpora lutea and also cells of the coexisting follicles including oocytes express V-1 mRNA and the protein, with apparent rank order of the expression: oocytes > luteal cells > follicular cells >> atretic follicular cells including oocytes. These data indicate the dynamic change in the V-1 gene expression in the ovarian steroidogenic cells and oocytes and suggest potential roles of the V-1 protein in ovarian functions including corpus luteum formation and folliculogenesis.
Song
S Y
SY
Mitsubishi Kasei Institute of Life Sciences, Machida, Tokyo 194, Japan.
Asakai
R
R
Kenmotsu
N
N
Taoka
M
M
Isobe
T
T
Yamakuni
T
T
D000818:Animals; D015152:Blotting, Northern; D015153:Blotting, Western; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D003338:Corpus Luteum / Q000502:physiology*; D005260:Female; D015870:Gene Expression; D018816:Genes, cdc / Q000235:genetics*; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D036341:Intercellular Signaling Peptides and Proteins; D009419:Nerve Tissue Proteins / Q000235:genetics*; D006080:Ovarian Follicle / Q000502:physiology*; D010053:Ovary / Q000166:cytology / Q000378:metabolism; D011555:Pseudopregnancy / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats
D006063:Chorionic Gonadotropin; D036341:Intercellular Signaling Peptides and Proteins; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; C065700:myotrophin