title: Sequence of a gene encoding a putative primary sigma factor from Borrelia burgdorferi strain B31.
abstract: Utilizing a polymerase chain reaction-based approach, the gene (rpoD) encoding the primary sigma factor from Borrelia burgdorferi strain B31 was cloned and sequenced. Nucleotide sequence analysis revealed an open reading frame (ORF) of 1632 bp (543 amino acids (aa), 63.7 kDa). Comparison with Escherichia coli sigma 70 and Bacillus subtilis sigma 43 showed a high degree of similarity in the aa sequences, especially for the regions that are known to be required for promoter recognition and core binding.
authors.affiliation: Department of Veterinary Medicine, National Taiwan University, Taipei, Republic of China.
authors.forename: C P
authors.initials: CP
authors.lastname: Tsai
authors.forename: M J
authors.initials: MJ
authors.lastname: Pan
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D015748:Borrelia burgdorferi Group / Q000737:chemistry / Q000235:genetics*; D003001:Cloning, Molecular; D017124:Conserved Sequence / Q000235:genetics; D017931:DNA Primers / Q000737:chemistry; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D017421:Sequence Analysis; D012689:Sequence Homology, Nucleic Acid; D012808:Sigma Factor / Q000737:chemistry / Q000235:genetics*
chemical_list: D017931:DNA Primers; D012808:Sigma Factor