Activity of the mitochondrial multiple conductance channel is independent of the adenine nucleotide translocator.
The functional relationship between the adenine nucleotide translocator (ANT) and the mitochondrial multiple conductance channel (MCC) was investigated using patch-clamp techniques. MCC activity with the same conductance, ion selectivity, voltage dependence, and peptide sensitivity could be reconstituted from inner membrane fractions derived from mitochondria of ANT-deficient and wild-type Saccharomyces cerevisiae. In addition, the MCC activity of mouse kidney mitoplasts was unaffected by carboxyatractyloside, a known inhibitor of ANT and inducer of a permeability transition. These results suggest that MCC activity is independent of ANT.
Lohret
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Division of Molecular Medicine, Wadsworth Center, New York State Department of Health, Albany, New York, 12201 0509, USA.
Murphy
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Drgoñ
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Kinnally
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D000595:Amino Acid Sequence; D000818:Animals; D001278:Atractyloside / Q000031:analogs & derivatives / Q000494:pharmacology; D003576:Electron Transport Complex IV / Q000737:chemistry; D004791:Enzyme Inhibitors / Q000494:pharmacology; D007425:Intracellular Membranes / Q000187:drug effects / Q000502:physiology*; D007473:Ion Channels / Q000187:drug effects / Q000502:physiology*; D007668:Kidney / Q000502:physiology; D007700:Kinetics; D008564:Membrane Potentials; D051379:Mice; D008928:Mitochondria / Q000502:physiology*; D000226:Mitochondrial ADP, ATP Translocases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D008969:Molecular Sequence Data; D018408:Patch-Clamp Techniques; D010455:Peptides / Q000138:chemical synthesis / Q000494:pharmacology*; D012441:Saccharomyces cerevisiae / Q000502:physiology
D004791:Enzyme Inhibitors; D007473:Ion Channels; D010455:Peptides; D001278:Atractyloside; D000226:Mitochondrial ADP, ATP Translocases; D003576:Electron Transport Complex IV; C003853:carboxyatractyloside