title: Effects of aminofluorene and acetylaminofluorene DNA adducts on transcriptional elongation by RNA polymerase II.
abstract: A prominent model for the mechanism of transcription-coupled DNA repair proposes that an arrested RNA polymerase directs the nucleotide excision repair complex to the transcription-blocking lesion. The specific role for RNA polymerase II in this mechanism can be examined by comparing the extent of polymerase arrest with the extent of transcription-coupled repair for a specific DNA lesion. Previously we reported that a cyclobutane pyrimidine dimer that is repaired preferentially in transcribed genes is a strong block to transcript elongation by RNA pol II (Donahue, B.A., Yin, S., Taylor, J.-S., Reines, D., and Hanawalt, P. C. (1994) Proc. Natl. Acad. Sci. U. S. A. 91, 8502-8506). Here we report the extent of RNA polymerase II arrest by the C-8 guanine DNA adduct formed by N-2-aminofluorene, a lesion that does not appear to be preferentially repaired. Templates for an in vitro transcription assay were constructed with either an N-2-aminofluorene adduct or the helix-distorting N-2-acetylaminofluorene adduct situated at a specific site downstream from the major late promoter of adenovirus. Consistent with the model for transcription-coupled repair, an aminofluorene adduct located on the transcribed strand was a weak pause site for RNA polymerase II. An acetylaminofluorene adduct located on the transcribed strand was an absolute block to transcriptional elongation. Either adduct located on the nontranscribed strand enhanced polymerase arrest at a nearby sequence-specific pause site.
authors.affiliation: Department of Biological Sciences, Stanford University, California 94305-5020, USA.
authors.forename: B A
authors.initials: BA
authors.lastname: Donahue
authors.forename: R P
authors.initials: RP
authors.lastname: Fuchs
authors.forename: D
authors.initials: D
authors.lastname: Reines
authors.forename: P C
authors.initials: PC
authors.lastname: Hanawalt
mesh_terms: D015073:2-Acetylaminofluorene / Q000633:toxicity*; D001483:Base Sequence; D004247:DNA / Q000187:drug effects; D018736:DNA Adducts; D005449:Fluorenes / Q000633:toxicity*; D008969:Molecular Sequence Data; D009153:Mutagens / Q000633:toxicity*; D012319:RNA Polymerase II / Q000037:antagonists & inhibitors / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D013698:Templates, Genetic; D014158:Transcription, Genetic
chemical_list: D018736:DNA Adducts; D005449:Fluorenes; D009153:Mutagens; D012333:RNA, Messenger; C012177:2-aminofluorene; D004247:DNA; D015073:2-Acetylaminofluorene; D012319:RNA Polymerase II