title: Androgens are not major down-regulators of androgen receptor levels during growth of the immature rat penis .
abstract: This study was undertaken to investigate the prevalent hypothesis that androgens are responsible for the organ-specific down-regulation of penile androgen receptors (ARs) and decline of penile growth in the rat during sexual maturation. Sexually immature male rats (21 days old) were castrated and treated for 3 days ("short-term"), with high doses of: (a) testosterone and the alpha-reductase inhibitor finasteride (T/F); (b) dihydrotestosterone (DHT); or (c) finasteride alone (F). Intact and castrate controls received vehicle only. PolyA + RNA was analysed by Northern blot hybridization and ARs were estimated in the penis and ventral prostates by (3-H)R-1881 binding in the cytosol. Short-term castration, with or without F, increased penile AR mRNA, whereas high doses of T/F and DHT reduced it considerably. Although penile cytosol AR concentration in the control castrates, with or without F, paralleled the AR mRNA rise, treatment with androgens left cytosol AR content per organ and AR concentration above those of the intact rat penis despite the drop in AR mRNA. A "long-term" treatment (10 days) on 19-day-old rats with either medium or high doses of T/F and DHT also failed to down-regulate penile cytosol ARs below the intact controls. Western blot analysis of penile cytosol AR levels confirmed these results. Block of pituitary FSH and LH release by a GnRH antagonist in castrates receiving T/F or DHT at high doses did not modify the response. In the case of intact rats, high doses of T/F or DHT actually increased penile cytosol AR content. No difference was observed between T/F and DHT effects. In contrast to what occurs during sexual maturation, the prostate ARs and growth rate responded to all treatments in a similar way to what was observed in the penis. Our results suggest that increases in serum T or DHT are not major factors in the physiological down-regulation of ARs and androgen-dependent growth in the rat corpora cavernosa.
authors.affiliation: Division of Urology, Department of Surgery, Harbor-UCLA Medical Center, University of California School of Medicine, Torrance 90509, USA.
authors.forename: R
authors.initials: R
authors.lastname: Shen
authors.forename: M C
authors.initials: MC
authors.lastname: Lin
authors.forename: F
authors.initials: F
authors.lastname: Sadeghi
authors.forename: R S
authors.initials: RS
authors.lastname: Swerdloff
authors.forename: J
authors.initials: J
authors.lastname: Rajfer
authors.forename: N F
authors.initials: NF
authors.lastname: Gonzalez-Cadavid
mesh_terms: D000728:Androgens / Q000502:physiology*; D000818:Animals; D042944:Cholestenone 5 alpha-Reductase; D003600:Cytosol / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D015536:Down-Regulation; D004791:Enzyme Inhibitors / Q000494:pharmacology; D018120:Finasteride / Q000494:pharmacology; D007987:Gonadotropin-Releasing Hormone / Q000037:antagonists & inhibitors; D007030:Hypothalamo-Hypophyseal System / Q000502:physiology; D008297:Male; D009919:Orchiectomy; D009929:Organ Size / Q000187:drug effects; D010088:Oxidoreductases / Q000037:antagonists & inhibitors; D010413:Penis / Q000254:growth & development* / Q000378:metabolism; D011467:Prostate / Q000254:growth & development / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D011944:Receptors, Androgen / Q000378:metabolism*; D012741:Sexual Maturation
chemical_list: D000728:Androgens; D004791:Enzyme Inhibitors; D012333:RNA, Messenger; D011944:Receptors, Androgen; D007987:Gonadotropin-Releasing Hormone; D018120:Finasteride; D010088:Oxidoreductases; D042944:Cholestenone 5 alpha-Reductase