title: Crystal structure of the T state of allosteric yeast chorismate mutase and comparison with the R state.
abstract: The crystal structure of the tyrosine-bound T state of allosteric yeast Saccharomyces cerevisiae chorismate mutase was solved by molecular replacement at a resolution of 2.8 angstroms using a monomer of the R-state structure as the search model. The allosteric inhibitor tyrosine was found to bind in the T state at the same binding site as the allosteric activator tryptophan binds in the R state, thus defining one regulatory binding site for each monomer. Activation by tryptophan is caused by the larger steric size of its side chain, thereby pushing apart the allosteric domain of one monomer and helix H8 of the catalytic domain of the other monomer. Inhibition is caused by polar contacts of tyrosine with Arg-75 and Arg-76 of one monomer and with Gly-141, Ser-142, and Thr-145 of the other monomer, thereby bringing the allosteric and catalytic domains closer together. The allosteric transition includes an 8 degree rotation of each of the two catalytic domains relative to the allosteric domains of each monomer (domain closure). Alternatively, this transition can be described as a 15 degree rotation of the catalytic domains of the dimer relative to each other.
authors.affiliation: Gibbs Chemical Laboratory, Harvard University, Cambridge, MA 02138, USA.
authors.forename: N
authors.initials: N
authors.lastname: Strater
authors.forename: K
authors.initials: K
authors.lastname: Hakansson
authors.forename: G
authors.initials: G
authors.lastname: Schnappauf
authors.forename: G
authors.initials: G
authors.lastname: Braus
authors.forename: W N
authors.initials: WN
authors.lastname: Lipscomb
mesh_terms: D000495:Allosteric Site / Q000235:genetics; D001665:Binding Sites / Q000235:genetics; D002826:Chorismate Mutase / Q000737:chemistry* / Q000235:genetics; D003460:Crystallization; D008958:Models, Molecular; D015394:Molecular Structure; D017354:Point Mutation; D011487:Protein Conformation; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics
chemical_list: D002826:Chorismate Mutase