Expression of CD44 variant exons 8-10 in gastric cancer.
The expression of CD44 variant containing variant exons 8-10 product (CD44v8-10) was studied by western blot analysis and immunohistochemistry in gastric cancers using a monoclonal antibody, 44-1V. On western blots, a single band of 130 kD was recognized in stomach cancer cell lines. CD44v8-10 expression, with reactivity localized in the cell membrane, was found in 65 (33.5%) of the 194 advanced gastric cancers. There was no correlation between CD44v8-10 immunoreactivity and serosal, lymphatic, or lymph node invasion. However, there was significant correlation with CD44v8-10 immunoreactivity and venous invasion. CD44v8-10-positive cancers were more frequently associated with hematogenous metastasis than those which were immunonegative. There was an inverse association between CD44v8-10 immunoreactivity and peritoneal dissemination, especially in diffuse type adenocarcinomas. These observations indicate that CD44v8-10 may play a role in the metastasis of gastric cancer.
Yamaguchi
A
A
First Department of Surgery, Fukui Medical School.
Saito
M
M
Gio
T
T
Iida
A
A
Takeuchi
K
K
Hirose
K
K
Nakagawara
G
G
Urano
T
T
Furukawa
K
K
Shiku
H
H
D000230:Adenocarcinoma / Q000235:genetics / Q000473:pathology; D000911:Antibodies, Monoclonal / Q000276:immunology; D000951:Antigens, Neoplasm / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology; D015153:Blotting, Western; D002277:Carcinoma / Q000235:genetics* / Q000473:pathology; D005091:Exons / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D018960:Hyaluronan Receptors / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology; D008207:Lymphatic Metastasis / Q000235:genetics; D009362:Neoplasm Metastasis; D009360:Neoplastic Cells, Circulating; D011993:Recombinant Fusion Proteins / Q000276:immunology; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D018960:Hyaluronan Receptors; D011993:Recombinant Fusion Proteins