Cell adhesion and integrin binding to recombinant human fibrillin-1.
Fibrillin-1 is a major constituent of tissue microfibrils that occur in most connective tissues, either in close association with or independent of elastin. To test possible cell-adhesive functions of this protein, we used recombinant human fibrillin-1 polypeptides produced in a mammalian expression system in cell attachment and solid-phase integrin binding assays. Fibrillin-1 polypeptides containing the single RGD sequence located in the fourth 8-cysteine domain, mediated distinct cell adhesion of a variety of cell lines and bound to purified integrin alphaVbeta3. Integrins alphaIIbbeta3, alpha5beta1, alpha2beta1 and alpha1beta1 did not interact with any of the recombinant fibrillin-1 peptides. Our results indicate a novel role for fibrillin-1 in cellular interactions mediated via an RGD motif that is appropriately exposed for recognition by integrin alphaVbeta3.
Pfaff
M
M
Max Planck Institut für Biochemie, Martinsried, Germany.
Reinhardt
D P
DP
Sakai
L Y
LY
Timpl
R
R
D000595:Amino Acid Sequence; D001665:Binding Sites; D002448:Cell Adhesion; D002460:Cell Line; D000071838:Fibrillin-1; D000071837:Fibrillins; D005340:Fibrinogen / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D016023:Integrins / Q000378:metabolism*; D008840:Microfilament Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D012680:Sensitivity and Specificity; D014407:Tumor Cells, Cultured; D019096:Vitronectin / Q000378:metabolism; D054392:Adipokines
D000071838:Fibrillin-1; D000071837:Fibrillins; D005340:Fibrinogen; D016023:Integrins; D008840:Microfilament Proteins; D010446:Peptide Fragments; D011994:Recombinant Proteins; D019096:Vitronectin; C000605722:FBN1 protein, human; D054392:Adipokines