Prevalence of mucosotropic human papillomaviruses in squamous-cell carcinoma of the head and neck.
The prevalence of mucosotropic human papillomavirus (HPV) DNA in 63 squamous-cell carcinomas (SCC) from different anatomic sites in the head and neck was determined by general primer-mediated polymerase chain reaction (GP-PCR). HPV DNA was detected in 20.6% of SCC. Additional type-specific PCR for HPV 6, 11, 16, 18, 31 and 33 demonstrated the presence of HPV 16 alone in these carcinomas. HPV 16 was also detected in normal epithelium from the resection margins of the majority of HPV-positive SCC. HPV status did not correlate with tumour site, whether primary or recurrent, TNM stage, metastases, degree of differentiation, smoking or alcohol history, fate or survival.
Snijders
P J
PJ
Department of Pathology, Section of Molecular Pathology, Free University Hospital, Amsterdam, The Netherlands.
Scholes
A G
AG
Hart
C A
CA
Jones
A S
AS
Vaughan
E D
ED
Woolgar
J A
JA
Meijer
C J
CJ
Walboomers
J M
JM
Field
J K
JK
D000428:Alcohol Drinking; D001483:Base Sequence; D002294:Carcinoma, Squamous Cell / Q000821:virology*; D017931:DNA Primers / Q000737:chemistry; D004279:DNA, Viral / Q000032:analysis; D006258:Head and Neck Neoplasms / Q000821:virology*; D006801:Humans; D008969:Molecular Sequence Data; D009061:Mouth Mucosa / Q000821:virology; D027383:Papillomaviridae / Q000472:pathogenicity*; D016133:Polymerase Chain Reaction; D012907:Smoking
D017931:DNA Primers; D004279:DNA, Viral