title: Gonadotropin hyperstimulation influences the 35S-methionine metabolism of mouse preimplantation embryos.
abstract: The effects of gonadotropin stimulation on mouse embryo uptake and incorporation of 35S-methionine were studied. We found that the uptake of 35S-methionine was reduced in embryos of stimulated females in both the two-cell and the blastocyst developmental stage. The incorporation of 35S-methionine into protein was not statistically significantly different between the embryos of stimulated and those of unstimulated females. Qualitatively, protein synthesis was equal in both groups as determined with one-dimensional SDS-PAGE. The results are discussed and we conclude that mouse embryo viability in vivo is decreased by ovarian stimulation.
authors.affiliation: Department of Gynecology and Obstetrics, University Hospital Nijmegen, The Netherlands.
authors.forename: A M
authors.initials: AM
authors.lastname: Wetzels
authors.forename: M T
authors.initials: MT
authors.lastname: Artz
authors.forename: H J
authors.initials: HJ
authors.lastname: Goverde
authors.forename: B A
authors.initials: BA
authors.lastname: Bastiaans
authors.forename: C J
authors.initials: CJ
authors.lastname: Hamilton
authors.forename: R
authors.initials: R
authors.lastname: Rolland
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000187:drug effects / Q000378:metabolism*; D005314:Embryonic and Fetal Development; D005260:Female; D006062:Gonadotropins / Q000494:pharmacology*; D008715:Methionine / Q000378:metabolism*; D051379:Mice; D014176:Protein Biosynthesis
chemical_list: D006062:Gonadotropins; D008715:Methionine