title: F-actin forms transient perinuclear shells at the mitosis-interphase transition.
abstract: Intermediate filaments and microtubules are known to be involved in establishing and maintaining nuclear shape. F-actin may also be involved in determining nuclear shape, since we have found it associated with reforming nuclei very briefly after cell division. We stained cells from vertebrate tissue cultures (3T3 and NRK-49F) and epidermal cells from an insect with rhodamine-phalloidin and Hoechst #33342 to localize F-actin in relation to the nucleus. We found that F-actin forms shells only around nuclei during reorganization in late mitosis and early interphase. We suggest that perinuclear F-actin shells may be generally present in eukaryotes, but that they are easily missed because of their delicacy and transience.
authors.affiliation: Department of Zoology, University of Western Ontario, London, Canada.
authors.forename: B H
authors.initials: BH
authors.lastname: Clubb
authors.forename: M
authors.initials: M
authors.lastname: Locke
mesh_terms: D016475:3T3 Cells / Q000737:chemistry* / Q000166:cytology / Q000502:physiology; D000199:Actins / Q000502:physiology*; D000818:Animals; D001562:Benzimidazoles; D002467:Cell Nucleus / Q000737:chemistry / Q000502:physiology / Q000648:ultrastructure; D000078404:Epidermal Cells; D004817:Epidermis / Q000737:chemistry; D005404:Fixatives; D005456:Fluorescent Dyes; D007399:Interphase / Q000502:physiology; D007668:Kidney / Q000166:cytology; D007915:Lepidoptera; D051379:Mice; D008938:Mitosis / Q000502:physiology*; D010590:Phalloidine; D051381:Rats; D012235:Rhodamines; D013194:Staining and Labeling
chemical_list: D000199:Actins; D001562:Benzimidazoles; D005404:Fixatives; D005456:Fluorescent Dyes; D012235:Rhodamines; D010590:Phalloidine; C017807:bisbenzimide ethoxide trihydrochloride