Determination of tryptophan and its kynurenine pathway metabolites in human serum by high-performance liquid chromatography with simultaneous ultraviolet and fluorimetric detection.
An isocratic reversed-phase high-performance liquid chromatographic method for the simultaneous determination of tryptophan and four metabolites of the kynurenine pathway (kynurenine, 3-hydroxykynurenine, kynurenic acid and 3-hydroxyanthranilic acid) in human serum is described. This new method, which uses both isocratic elution and two on-line connected programmable ultraviolet and spectrofluorimetric detectors, allows the determination of these metabolites, in the physiological ranges, with satisfying specificity and sensitivity within 30 min.
Hervé
C
C
Service de Biochimie, Hôpital Beaujon 100, Clichy, France.
Beyne
P
P
Jamault
H
H
Delacoux
E
E
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D007737:Kynurenine / Q000097:blood*; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D014364:Tryptophan / Q000097:blood*
D007737:Kynurenine; D014364:Tryptophan