title: Expression and regulation of Cek-8, a cell to cell signalling receptor in developing chick limb buds.
abstract: The Eph-related receptor tyrosine kinase gene, Cek-8, is expressed in mesenchyme at the tip of chick limb buds, with high levels of transcripts posteriorly and apically but fading out anteriorly. Expression of Cek-8 in distal mesenchyme is regulated by apical ridge- and FGF-polarising signals and retinoic acid, and is uniform across the anteroposterior axis in talpid3 mutants. These data indicate that Cek-8 expression responds to regulatory signals during limb patterning and suggest that this receptor tyrosine kinase may have a role in coordinating responses to signals in the progress zone of early buds. Later on in limb development, Cek-8 expression is associated with cell condensations that form tendons and their attachments to cartilage rudiments and then in developing feather buds.
authors.affiliation: Division of Developmental Neurobiology, National Institute for Medical Research, London, UK.
authors.forename: K
authors.initials: K
authors.lastname: Patel
authors.forename: R
authors.initials: R
authors.lastname: Nittenberg
authors.forename: D
authors.initials: D
authors.lastname: D'Souza
authors.forename: C
authors.initials: C
authors.lastname: Irving
authors.forename: D
authors.initials: D
authors.lastname: Burt
authors.forename: D G
authors.initials: DG
authors.lastname: Wilkinson
authors.forename: C
authors.initials: C
authors.lastname: Tickle
mesh_terms: D000818:Animals; D019485:Bone Morphogenetic Proteins; D002450:Cell Communication; D002642:Chick Embryo; D005241:Feathers / Q000196:embryology; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects / Q000502:physiology*; D006133:Growth Substances / Q000494:pharmacology; D018878:Limb Buds / Q000166:cytology / Q000254:growth & development / Q000502:physiology*; D008648:Mesoderm / Q000737:chemistry; D051379:Mice; D008810:Mice, Inbred C57BL; D008863:Microspheres; D009154:Mutation; D009479:Neuropeptides / Q000235:genetics*; D011506:Proteins / Q000494:pharmacology; D012333:RNA, Messenger / Q000032:analysis; D020794:Receptor Protein-Tyrosine Kinases / Q000235:genetics*; D036122:Receptor, EphA4; D013710:Tendons / Q000737:chemistry / Q000196:embryology; D014212:Tretinoin / Q000494:pharmacology
chemical_list: D019485:Bone Morphogenetic Proteins; D006133:Growth Substances; D009479:Neuropeptides; D011506:Proteins; D012333:RNA, Messenger; D016222:Fibroblast Growth Factor 2; D014212:Tretinoin; D020794:Receptor Protein-Tyrosine Kinases; D036122:Receptor, EphA4