title: Functional disturbance of naive T lymphocytes in very high IgE producers: depletion of interleukin-4-induced interleukin-4-producing cells.
abstract: We examined the capacity of T cells from normal individuals and allergic patients with very high IgE to differentiate into interleukin-4(IL-4)-producing cells in vitro. T cells incubated with anti-CD3 monoclonal antibody plus IL-4 or plus anti-IL-4 antibody in the presence of antigen-presenting cells for 7 days were restimulated and their capacity to express IL-4 mRNA was examined by RT-PCR. In T cells from normal individuals, there was a marked increase in the expression of IL-4 mRNA following the addition of IL-4. After fractionation of normal T cells into naive T (CD45RA+) and memory T (CD45RO+) cells, induction of the increase of IL-4 mRNA was restricted to the naive T cell population. In contrast, in T cells from allergic patients, the stimulation of whole or naive T cells with anti-CD 3 monoclonal antibody in the presence of IL-4 induced much less IL-4 mRNA. These findings suggest the presence of a functional abnormality in IL-4-dependent development of IL-4-producing T cells in the peripheral-blood naive T cells from allergic patients.
authors.affiliation: Department of Internal Medicine III, Osaka University Medical School, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Ochi
authors.forename: T
authors.initials: T
authors.lastname: Tanaka
authors.forename: Y
authors.initials: Y
authors.lastname: Katada
authors.forename: T
authors.initials: T
authors.lastname: Naka
authors.forename: M
authors.initials: M
authors.lastname: Aitani
authors.forename: S
authors.initials: S
authors.lastname: Hashimoto
authors.forename: K
authors.initials: K
authors.lastname: Maeda
authors.forename: K
authors.initials: K
authors.lastname: Toyoshima
authors.forename: T
authors.initials: T
authors.lastname: Igarashi
authors.forename: M
authors.initials: M
authors.lastname: Suemura
authors.forename: T
authors.initials: T
authors.lastname: Kishimoto
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology; D001483:Base Sequence; D002454:Cell Differentiation / Q000187:drug effects / Q000276:immunology; D017637:Clonal Deletion / Q000187:drug effects; D006801:Humans; D006967:Hypersensitivity / Q000276:immunology*; D007073:Immunoglobulin E / Q000096:biosynthesis*; D007156:Immunologic Memory / Q000187:drug effects; D015847:Interleukin-4 / Q000096:biosynthesis* / Q000235:genetics / Q000502:physiology*; D008213:Lymphocyte Activation / Q000187:drug effects; D018655:Lymphocyte Count; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000096:biosynthesis / Q000187:drug effects; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology* / Q000378:metabolism
chemical_list: D000276:Adjuvants, Immunologic; D012333:RNA, Messenger; D015847:Interleukin-4; D007073:Immunoglobulin E