Application of accelerator mass spectrometry (AMS) for high-sensitivity measurements of 14CO2 in long-term studies of fat metabolism.
Long-term measurements of 14C in CO2 expired after ingestion of 14C-labelled triolein were performed using accelerator mass spectrometry (AMS). About 30% of a given amount of 14C-labelled triolein was catabolized rapidly, while the remaining 70% had a very slow turnover. The study shows the potential of the AMS technique for the study of the long-term biokinetics of 14C-labelled pharmaceuticals. The AMS technique allows the administered activity to be reduced by several orders of magnitude without compromising the study. It may also allow studies of rare drug metabolites.
Stenström
K
K
Department of Nuclear Physics, University of Lund, Sweden.
Leide-Svegborn
S
S
Erlandsson
B
B
Hellborg
R
R
Mattsson
S
S
Nilsson
L E
LE
Nosslin
B
B
Skog
G
G
Wiebert
A
A
D000328:Adult; D002245:Carbon Dioxide / Q000032:analysis*; D002250:Carbon Radioisotopes; D004041:Dietary Fats; D006801:Humans; D050356:Lipid Metabolism; D008297:Male; D013058:Mass Spectrometry / Q000379:methods; D008875:Middle Aged; D013997:Time Factors; D014304:Triolein / Q000378:metabolism*
D002250:Carbon Radioisotopes; D004041:Dietary Fats; D014304:Triolein; D002245:Carbon Dioxide