title: Rac-dependent and -independent pathways mediate growth factor-induced Ca2+ influx.
abstract: We report that expressing interfering mutants of the small Ras-related GTPase Rac, using either recombinant vaccinia virus or stable DNA transfection, eliminates epidermal growth factor-induced Ca2+ signaling, without affecting Ca2+ mobilization or influx from G protein-coupled receptors. Platelet-derived growth factor-dependent Ca2+ influx, however, is only partly sensitive to dominant negative Rac proteins. Thus, whereas epidermal growth factor-induced Ca2+ influx is completely mediated by Rac proteins, platelet-derived growth factor-induced Ca2+ influx involves Rac-dependent and -independent signaling pathways.
authors.affiliation: Laboratory for Physiological Chemistry, Utrecht University, Universiteitsweg 100, NL-3584 CG Utrecht, The Netherlands.
authors.forename: M P
authors.initials: MP
authors.lastname: Peppelenbosch
authors.forename: L G
authors.initials: LG
authors.lastname: Tertoolen
authors.forename: A M
authors.initials: AM
authors.lastname: de Vries-Smits
authors.forename: R G
authors.initials: RG
authors.lastname: Qiu
authors.forename: L
authors.initials: L
authors.lastname: M'Rabet
authors.forename: M H
authors.initials: MH
authors.lastname: Symons
authors.forename: S W
authors.initials: SW
authors.lastname: de Laat
authors.forename: J L
authors.initials: JL
authors.lastname: Bos
mesh_terms: D000818:Animals; D002118:Calcium / Q000502:physiology*; D002478:Cells, Cultured; D004815:Epidermal Growth Factor / Q000502:physiology; D066246:ErbB Receptors / Q000502:physiology*; D019204:GTP-Binding Proteins / Q000502:physiology*; D006367:HeLa Cells; D006632:Histamine / Q000494:pharmacology; D006801:Humans; D010982:Platelet-Derived Growth Factor / Q000502:physiology; D051381:Rats; D017479:Receptors, Platelet-Derived Growth Factor / Q000502:physiology*; D011994:Recombinant Proteins; D015398:Signal Transduction; D014616:Vaccinia virus; D020744:rac GTP-Binding Proteins; D020741:rho GTP-Binding Proteins
chemical_list: D010982:Platelet-Derived Growth Factor; D011994:Recombinant Proteins; D004815:Epidermal Growth Factor; D006632:Histamine; D066246:ErbB Receptors; D017479:Receptors, Platelet-Derived Growth Factor; D019204:GTP-Binding Proteins; D020744:rac GTP-Binding Proteins; D020741:rho GTP-Binding Proteins; D002118:Calcium