Perturbation of nifT expression in Klebsiella pneumoniae has limited effect on nitrogen fixation.
In the nitrogenase system of Klebsiella pneumoniae, nifT is located between nifDK, the structural genes for dinitrogenase, and nifY, whose product is involved in nitrogenase maturation. It is, therefore, a reasonable hypothesis that the NifT protein might also have a role in the maturation of nitrogenase. However, the phenotypic characterization of nifT and nifT-overexpressing strains for effects on the regulation, maturation, and activity of nitrogenase identified no properties that were distinct from those of the wild type. We conclude that the K. pneumoniae NifT protein is not essential for nitrogen fixation under the conditions examined.
Simon
H M
HM
Department of Bacteriology, University of Wisconsin-Madison 53706, USA.
Homer
M J
MJ
Roberts
G P
GP
D001426:Bacterial Proteins / Q000235:genetics*; D005798:Genes, Bacterial; D007711:Klebsiella pneumoniae / Q000235:genetics*; D016296:Mutagenesis; D009586:Nitrogen Fixation / Q000235:genetics*; D009591:Nitrogenase / Q000096:biosynthesis; D017384:Sequence Deletion
D001426:Bacterial Proteins; D009591:Nitrogenase