Selective extraction of beta-blockers from biological fluids by column-switching high-performance liquid chromatography using an internal-surface phenylboronic acid precolumn.
A column-switching HPLC method using an internal-surface phenylboronic acid precolumn for the selective extraction of beta-blockers from biological fluids has been developed. Filtered urine and plasma samples (50 microliters) were injected onto the precolumn equilibrated with methanol-0.05 M disodium hydrogenphosphate (5:95, v/v). After the precolumn had been washed briefly, the selectively retained beta-blockers were eluted with methanol-0.05 M phosphate buffer (pH 2.0) and transferred to a reversed-phase analytical column, on which they were then separated. Even after exposure to at least 160 injections of non-treated urine and plasma samples, the retention efficiency of the precolumn was maintained with no increase in back pressure. Quantitative recoveries and good reproducibility were demonstrated with pindolol.
Ohta
T
T
Faculty of Pharmaceutical Sciences, Science University of Tokyo, Japan.
Niida
S
S
Nakamura
H
H
D000319:Adrenergic beta-Antagonists / Q000097:blood* / Q000652:urine*; D001897:Boronic Acids; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010869:Pindolol / Q000097:blood* / Q000652:urine*; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet
D000319:Adrenergic beta-Antagonists; D001897:Boronic Acids; D010869:Pindolol; C010686:benzeneboronic acid