Nested-polymerase chain reaction for the detection of Helicobacter pylori infection with novel primers designed by sequence analysis of urease A gene in clinically isolated bacterial strains.
We have established a highly sensitive semi-nested PCR assay for the detection of H. pylori infection using gastric juice samples, which can be aspirated with disposable nasogastric tubes. The primers targeting H. pylori urease A gene were designed based on the sequence conservation analysis of sixteen H. pylori strains isolated from Japanese patients. The efficacy of the PCR assay, designated as the URA-PCR, was confirmed by in vitro and in vivo assessments. Its sensitivity was 97.5% in the gastric juice samples aspirated from forty patients with proven H. pylori infection, and was significantly higher than that obtained with previously described PCR assays.
Kawamata
O
O
Center for Molecular Biology and Cytogenetics, SRL Inc., Tokyo, Japan.
Yoshida
H
H
Hirota
K
K
Yoshida
A
A
Kawaguchi
R
R
Shiratori
Y
Y
Omata
M
M
D001483:Base Sequence; D017931:DNA Primers; D005750:Gastric Juice / Q000382:microbiology; D005798:Genes, Bacterial; D016481:Helicobacter Infections / Q000175:diagnosis*; D016480:Helicobacter pylori / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012689:Sequence Homology, Nucleic Acid; D014510:Urease / Q000235:genetics*
D017931:DNA Primers; D014510:Urease