title: Multiple transduction pathways regulate the sodium-extrusion gene PMR2/ENA1 during salt stress in yeast.
abstract: The yeast PMR2/ENA1 gene encodes an ATPase involved in sodium extrusion and induced by NaCl. At low salt concentrations (0.3 M) induction is mediated by the HOG-MAP kinase pathway, a system activated by non-specific osmotic stress. At high salt concentrations (0.8 M) induction is mediated by the protein phosphatase calcineurin and is specific for sodium. Protein kinase A and Sis2p/Hal3p modulate PMR2/ENA1 expression as negative and positive factors, respectively but Sis2p/Hal3p does not participate in the transduction of the salt signal. Salt stress decreases the level of cAMP and the resulting decrease in protein kinase A activity may contribute to HOG-mediated induction.
authors.affiliation: Instituto de Biologia Molecular y Celular de Plantas, Valencia, Spain.
authors.forename: J A
authors.initials: JA
authors.lastname: Márquez
authors.forename: R
authors.initials: R
authors.lastname: Serrano
mesh_terms: D000251:Adenosine Triphosphatases / Q000235:genetics*; D019703:Calcineurin; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000502:physiology; D002148:Calmodulin-Binding Proteins / Q000037:antagonists & inhibitors / Q000502:physiology; D027682:Cation Transport Proteins; D018797:Cell Cycle Proteins; D017868:Cyclic AMP-Dependent Protein Kinases / Q000502:physiology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005656:Fungal Proteins / Q000502:physiology; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects*; D005800:Genes, Fungal / Q000235:genetics; D017930:Genes, Reporter; D017136:Ion Transport; D009997:Osmotic Pressure; D010749:Phosphoprotein Phosphatases / Q000037:antagonists & inhibitors / Q000502:physiology; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins; D015398:Signal Transduction / Q000502:physiology*; D012964:Sodium / Q000378:metabolism; D012965:Sodium Chloride / Q000494:pharmacology*; D000254:Sodium-Potassium-Exchanging ATPase; D016559:Tacrolimus / Q000494:pharmacology; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
chemical_list: D002148:Calmodulin-Binding Proteins; D027682:Cation Transport Proteins; D018797:Cell Cycle Proteins; C086375:ENA1 protein, S cerevisiae; D004791:Enzyme Inhibitors; D005656:Fungal Proteins; D011993:Recombinant Fusion Proteins; C092936:SIS2 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D012965:Sodium Chloride; D012964:Sodium; D017868:Cyclic AMP-Dependent Protein Kinases; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D019703:Calcineurin; D010749:Phosphoprotein Phosphatases; D001616:beta-Galactosidase; D000251:Adenosine Triphosphatases; D000254:Sodium-Potassium-Exchanging ATPase; D016559:Tacrolimus