A concurrent human herpesvirus-6 infection renders two human hematopoietic progenitor (TF-1 and KG-1) cell lines susceptible to human immunodeficiency virus type-1.
In human immunodeficiency virus type-1 (HIV-1) infected individuals, CD34+ hematopoietic stem/progenitor cells are profoundly impaired in their proliferation/differentiation capacities. The bulk of the available experimental evidence seems to indicate that hematopoietic progenitors are not susceptible to HIV-1 infection and their defects seem rather the consequence of direct or indirect negative influences of HIV-1-specific soluble proteins released by productively infected accessory cells. We have now shown that in the presence of a concurrent human herpesvirus-6 infection, two hematopoietic (TF-1 [erythromyeloid] and KG-1 [lymphomyeloid]) progenitor cell lines and human CD34+ hematopoietic progenitors isolated from the bone marrow of normal donors, became susceptible to HIV-1 infection and permissive to HIV-1 replication, although with a limited virus yield. These results suggest a further possible mechanism leading to hematopoietic derangement in HIV-1-infected subjects and may help to clarify the controversial issue of the susceptibility of human hematopoietic progenitors to HIV-1 infection.
Furlini
G
G
Institute of Microbiology of the University of Bologna, Italy.
Vignoli
M
M
Ramazzotti
E
E
Re
M C
MC
Visani
G
G
La Placa
D000911:Antibodies, Monoclonal / Q000494:pharmacology; D001483:Base Sequence; D001854:Bone Marrow Cells; D015704:CD4 Antigens / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D002460:Cell Line; D018920:Coculture Techniques; D004198:Disease Susceptibility; D015536:Down-Regulation; D015967:Gene Expression Regulation, Viral; D015497:HIV-1 / Q000472:pathogenicity* / Q000502:physiology; D006412:Hematopoietic Stem Cells / Q000821:virology*; D015654:Herpesvirus 6, Human / Q000502:physiology*; D006801:Humans; D015459:Leukemia-Lymphoma, Adult T-Cell / Q000473:pathology; D008969:Molecular Sequence Data; D014407:Tumor Cells, Cultured; D015854:Up-Regulation; D014779:Virus Replication
D000911:Antibodies, Monoclonal; D015704:CD4 Antigens