title: Characterization of nuclear structures in cells infected with herpes simplex virus type 1 in the absence of viral DNA replication.
abstract: Herpes simplex virus type 1 DNA replication occurs in nuclear domains termed replication compartments, which are areas of viral single-stranded DNA-binding protein (UL29) localization (M.P. Quinlan, L. B. Chen, and D. M. Knipe, Cell 36:857-868). In the presence of herpesvirus-specific polymerase inhibitors, UL29 localizes to punctate nuclear foci called prereplicative sites. Using versions of the helicase-primase complex proteins containing short peptide epitopes which can be detected in an immunofluorescence assay, we have found that the helicase-primase complex localizes to prereplicative sites and replication compartments. To determine if prereplicative site formation is dependent upon these and other essential viral replication proteins, we have studied UL29 localization in cells infected with replication-defective viruses. Cells infected with viruses that fail to express one of the three helicase-primase subunits or the origin-binding protein show a diffuse nuclear staining for UL29. However, in the presence of polymerase inhibitors, mutant-infected cells contain UL29 in prereplicative sites. Replication-defective viruses containing subtle mutations in the helicase or origin-binding proteins behaved identically to their null mutant counterparts. In contrast, cells infected with viral mutants which fail to express the polymerase protein contain prereplicative sites in the absence and presence of polymerase inhibitors. We propose that active viral polymerase prevents the formation of prereplicative sites. Models of the requirement of essential viral replication proteins in the assembly of prereplicative sites are presented.
authors.affiliation: Department of Microbiology, University of Connecticut Health Center, Farmington 06030, USA.
authors.forename: C J
authors.initials: CJ
authors.lastname: Lukonis
authors.forename: S K
authors.initials: SK
authors.lastname: Weller
mesh_terms: D000818:Animals; D001483:Base Sequence; D002467:Cell Nucleus / Q000821:virology*; D002522:Chlorocebus aethiops; D004265:DNA Helicases / Q000235:genetics / Q000378:metabolism; D019915:DNA Primase; D004261:DNA Replication; D004279:DNA, Viral / Q000096:biosynthesis*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D004259:DNA-Directed DNA Polymerase / Q000235:genetics; D003673:Defective Viruses / Q000502:physiology; D000939:Epitopes; D018259:Herpesvirus 1, Human / Q000235:genetics* / Q000502:physiology; D006801:Humans; D008969:Molecular Sequence Data; D010746:Phosphonoacetic Acid / Q000494:pharmacology; D017354:Point Mutation; D014709:Vero Cells; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism; D014779:Virus Replication
chemical_list: D004279:DNA, Viral; D004268:DNA-Binding Proteins; D000939:Epitopes; C045462:ICP8 protein, Simplexvirus; D014764:Viral Proteins; C064565:UL9 protein, Human herpesvirus 1; D019915:DNA Primase; D004259:DNA-Directed DNA Polymerase; C062021:helicase-primase, Human herpesvirus 1; D004265:DNA Helicases; D010746:Phosphonoacetic Acid