Identification of multiple plasmids released from recombinant genomes of Hansenula polymorpha by transformation of Escherichia coli.
Total DNAs isolated from two Hansenula polymorpha (Pichia angusta) strains having chromosomal single or tandem multiple integrations of a pUC18-derived expression plasmid produced Escherichia coli transformants which contained plasmids of different size and/or organization than that of the expression plasmid. Evidence that plasmid-like structures are formed in H. polymorpha and that their formation is stimulated by DNA damage is presented in this study.
Graupner
S
S
Fachbereich Biologie, Universität Oldenburg, Germany.
Wackernagel
W
W
D004926:Escherichia coli / Q000235:genetics*; D010843:Pichia / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D014169:Transformation, Bacterial