Cloning, sequencing, and expression of a gene from Campylobacter jejuni encoding a protein (Omp18) with similarity to peptidoglycan-associated lipoproteins.
A Campylobacter jejuni genomic plasmid library was screened with antiserum generated against whole C. jejuni, revealing two immunoreactive clones. Sequence analysis of the recombinant plasmids revealed a common open reading frame of 498 nucleotides encoding a protein of 165 amino acids with a calculated molecular mass of 18,018 Da. The recombinant product partitioned to the outer membrane fractions of Escherichia coli transformants and has been designated Omp18. The deduced amino acid sequence of the cloned C. jejuni gene exhibits considerable similarity to peptidoglycan-associated lipoproteins from other gram-negative bacteria.
Konkel
M E
ME
Department of Microbiology, Washington State University, Pullman 99164, USA. Konkel@mail.wsu.edu
Mead
D J
DJ
Cieplak
W
W
D000595:Amino Acid Sequence; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics* / Q000276:immunology; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology; D001483:Base Sequence; D002169:Campylobacter Infections / Q000276:immunology / Q000517:prevention & control; D016123:Campylobacter jejuni / Q000235:genetics* / Q000276:immunology; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D006090:Gram-Negative Bacteria / Q000235:genetics; D006801:Humans; D008074:Lipoproteins / Q000235:genetics / Q000276:immunology; D008969:Molecular Sequence Data; D010457:Peptidoglycan / Q000235:genetics / Q000276:immunology; D017386:Sequence Homology, Amino Acid
D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D008074:Lipoproteins; D010457:Peptidoglycan; C097965:outer membrane protein p18, Campylobacter