Use of immobilized synthetic peptides for the identification of contact sites between human interleukin-6 and its receptor.
Synthetic peptides immobilized on cellulose membranes proved to be a powerful tool for the identification of sites in the cytokine IL-6 involved in receptor binding. Similarly, a region in the extracellular part of the IL-6 receptor which is important for interaction with its ligand was identified.
Weiergräber
O
O
Institut für Biochemie der RWTH Aachen, Germany.
Schneider-Mergener
J
J
Grötzinger
J
J
Wollmer
A
A
Küster
A
A
Exner
M
M
Heinrich
P C
PC
D000595:Amino Acid Sequence; D015703:Antigens, CD / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D001665:Binding Sites; D003001:Cloning, Molecular; D004926:Escherichia coli; D006801:Humans; D015850:Interleukin-6 / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D008961:Models, Structural; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry; D017433:Protein Structure, Secondary; D018123:Receptors, Interleukin / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D019947:Receptors, Interleukin-6; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism
D015703:Antigens, CD; D015850:Interleukin-6; D010455:Peptides; D018123:Receptors, Interleukin; D019947:Receptors, Interleukin-6; D011994:Recombinant Proteins