title: In vivo expression of a single viral DNA-binding protein generates systemic lupus erythematosus-related autoimmunity to double-stranded DNA and histones.
abstract: Although the origin of autoimmune antibodies to double-stranded DNA is not known, the variable-region structures of such antibodies indicate that they are produced in response to antigen-selective stimulation. In accordance with this, results from experiments using artificial complexes of DNA and DNA-binding polypeptides for immunizations have indicated that DNA may induce these antibodies. Hence, the immunogenicity of DNA in vivo may depend upon other structures or processes that may render DNA immunogenic. We report that in vivo expression of a single DNA-binding protein, the polyoma virus T antigen, is sufficient to initiate production of anti-double-stranded DNA and anti-histone antibodies but not a panel of other autoantigens. Expression of a mutant, non-DNA-binding T antigen did result in strong production of antibodies to the T antigen, but only borderline levels of antibodies to DNA and no detectable antibodies to histones. Nonexpressing plasmid DNA containing the complete cDNA sequence for T antigen did not evoke such immune responses, indicating that DNA by itself is not immunogenic in vivo. The results represent a conceptual advance in understanding a potential molecular basis for initiation of autoimmunity in systemic lupus erythematosus.
authors.affiliation: Department of Virology, University of Tromsø, Norway.
authors.forename: U
authors.initials: U
authors.lastname: Moens
authors.forename: O M
authors.initials: OM
authors.lastname: Seternes
authors.forename: A W
authors.initials: AW
authors.lastname: Hey
authors.forename: Y
authors.initials: Y
authors.lastname: Silsand
authors.forename: T
authors.initials: T
authors.lastname: Traavik
authors.forename: B
authors.initials: B
authors.lastname: Johansen
authors.forename: O P
authors.initials: OP
authors.lastname: Rekvig
mesh_terms: D016475:3T3 Cells; D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000096:biosynthesis* / Q000276:immunology; D001323:Autoantibodies / Q000096:biosynthesis*; D001324:Autoantigens / Q000276:immunology; D015551:Autoimmunity; D001483:Base Sequence; D004247:DNA / Q000276:immunology*; D017931:DNA Primers; D018076:DNA, Complementary; D004268:DNA-Binding Proteins / Q000096:biosynthesis* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D006657:Histones / Q000276:immunology*; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D010957:Plasmids; D016133:Polymerase Chain Reaction / Q000379:methods; D011401:Promoter Regions, Genetic
chemical_list: D000952:Antigens, Polyomavirus Transforming; D001323:Autoantibodies; D001324:Autoantigens; D017931:DNA Primers; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D006657:Histones; D004247:DNA