Peptide synthesis using unprotected peptides through orthogonal coupling methods.
We describe an approach to the synthesis of peptides from segments bearing no protecting groups through an orthogonal coupling method to capture the acyl segment as a thioester that then undergoes an intramolecular acyl transfer to the amine component with formation of a peptide bond. Two orthogonal coupling methods to give the covalent ester intermediate were achieved by either a thiol-thioester exchange mediated by a trialkylphosphine and an alkylthiol or a thioesterification by C alpha-thiocarboxylic acid reacting with a beta-bromo amino acid. With this approach, unprotected segments ranging from 4 to 37 residues were coupled to aqueous solution to give free peptides up to 54 residues long with high efficiency.
Tam
J P
JP
Department of Microbiology and Immunology, Vanderbilt University, Nashville, TN 37232-2363, USA.
Lu
Y A
YA
Liu
C F
CF
Shao
J
J
D000215:Acylation; D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid; D003545:Cysteine; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000138:chemical synthesis / Q000737:chemistry; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry; D010720:Phosphines; D012996:Solutions; D013438:Sulfhydryl Compounds
D007202:Indicators and Reagents; D009842:Oligopeptides; D010455:Peptides; D010720:Phosphines; D012996:Solutions; D013438:Sulfhydryl Compounds; D003545:Cysteine