title: Activation of yeast protein kinase C by Rho1 GTPase.
abstract: We have investigated the role of the essential Rho1 GTPase in cell integrity signaling in budding yeast. Conditional rho1 mutants display a cell lysis defect that is similar to that of mutants in the cell integrity signaling pathway mediated by protein kinase C (Pkc1), which is suppressed by overexpression of Pkc1.rho1 mutants are also impaired in pathway activation in response to growth at elevated temperature. Pkc1 co-immunoprecipitates with Rho1 in yeast extracts, and recombinant Rho1 associates with Pkc1 in vitro in a GTP-dependent manner. Recombinant Rho1 confers upon Pkc1 the ability to be stimulated by phosphatidylserine, indicating that Rho1 controls signal transmission through Pkc1.
authors.affiliation: Department of Biochemisty, Johns Hopkins University School of Public Health, Baltimore, Maryland 21205, USA.
authors.forename: Y
authors.initials: Y
authors.lastname: Kamada
authors.forename: H
authors.initials: H
authors.lastname: Qadota
authors.forename: C P
authors.initials: CP
authors.lastname: Python
authors.forename: Y
authors.initials: Y
authors.lastname: Anraku
authors.forename: Y
authors.initials: Y
authors.lastname: Ohya
authors.forename: D E
authors.initials: DE
authors.lastname: Levin
mesh_terms: D018797:Cell Cycle Proteins / Q000302:isolation & purification / Q000378:metabolism; D004789:Enzyme Activation; D020558:GTP Phosphohydrolases / Q000302:isolation & purification / Q000378:metabolism*; D019204:GTP-Binding Proteins / Q000302:isolation & purification / Q000378:metabolism*; D006160:Guanosine Triphosphate / Q000378:metabolism; D008954:Models, Biological; D016297:Mutagenesis, Site-Directed; D011493:Protein Kinase C / Q000302:isolation & purification / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000302:isolation & purification / Q000378:metabolism; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D029701:Saccharomyces cerevisiae Proteins; D013696:Temperature; D020846:cdc42 GTP-Binding Protein, Saccharomyces cerevisiae; D020741:rho GTP-Binding Proteins
chemical_list: D018797:Cell Cycle Proteins; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; D029701:Saccharomyces cerevisiae Proteins; D006160:Guanosine Triphosphate; D011493:Protein Kinase C; D020558:GTP Phosphohydrolases; D019204:GTP-Binding Proteins; C051302:RHO1 protein, S cerevisiae; D020846:cdc42 GTP-Binding Protein, Saccharomyces cerevisiae; D020741:rho GTP-Binding Proteins