Determination of dietary fiber and carbohydrate composition of foods by a modified version of the Englyst method.
A modified version of the Englyst procedure for determination of dietary fiber and carbohydrate fractions of foods is described. The method is based on hydrolysis of separated fractions and capillary gas chromatographic (GC) analysis of derivatized monomer sugars, UV photometry of uronic acids, gravimetric determination of residue, and analysis of nitrogen content of the residue by using a C, H, N elemental analyzer. Modifications of fractionation, derivatization, and GC procedures are discussed. Rigorous sample preparation is required for reproducibility of 200 mg test portions. Contaminants interfering with chromatography must be minimized. Results for reference samples (wheat bran and Buerre Bosc pears) and white and whole meal bread agree with literature values.
Lichon
M J
MJ
University of Tasmania, Department of Plant Science, Hobart 7001, Australia.
James
K W
KW
D001939:Bread / Q000032:analysis; D002849:Chromatography, Gas / Q000379:methods; D004040:Dietary Carbohydrates / Q000032:analysis*; D004043:Dietary Fiber / Q000032:analysis*; D005504:Food Analysis / Q000379:methods* / Q000706:statistics & numerical data; D005638:Fruit / Q000737:chemistry; D006868:Hydrolysis; D009584:Nitrogen / Q000032:analysis; D012680:Sensitivity and Specificity; D014908:Triticum / Q000737:chemistry; D014574:Uronic Acids / Q000032:analysis
D004040:Dietary Carbohydrates; D004043:Dietary Fiber; D014574:Uronic Acids; D009584:Nitrogen