Human monoclonal antibody against colon cancer.
The purpose of the study was to produce human monoclonal antibodies (hMcAb) against human colon cancer for use in radioimmunoimaging. Human-mouse heterohybridomas were developed by fusing SHM-D33 mouse-human hybrid heteromyeloma cells with human lymphocytes from colon cancer tumor-draining lymph nodes. The hybridomas capable of secreting human monoclonal antibodies were screened by using human colon cancer cell lines and pathological biopsies with ELISA and immunohistochemical methods. hMcAb clone H11 was selected for a large-scale antibody production, which was purified from mouse ascites. Biodistribution study demonstrated that specific uptake of 125I-hMcAb H11 by human colon cancer xenografts was significantly higher than by normal tissues. Radioimmunoimaging of human colon cancer xenografts exhibited distinct tumor visualization during the period of 72-96 hr after intraperitoneal injection of 125I-hMcAb H11. The development of human monoclonal antibodies such as hMcAb H11 may be useful for radioimmunodetection and therapy of colon cancer.
Yao
C Z
CZ
Department of Surgery, University of Texas Medical Branch, Galveston 77555-0722, USA.
Ishizuka
J
J
Bold
R J
RJ
Sperling
H E
HE
Townsend
C M
CM
Thompson
J C
JC
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D003110:Colonic Neoplasms / Q000000981:diagnostic imaging / Q000276:immunology*; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D011877:Radionuclide Imaging; D013997:Time Factors; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured / Q000276:immunology
D000911:Antibodies, Monoclonal