Primary structure of a cysteine proteinase inhibitor from the fruit of avocado (Persea americana Mill).
The complete amino acid sequence of a proteinaceous cysteine proteinase inhibitor from the fruit of avocado (avocado cystatin) is presented. The protein consists of 100 amino acid residues and has a molecular mass of 11,300 Da. Comparison of this sequence with sequences of plant cysteine proteinase inhibitors (phytocystatins), including oryzacystatins I and II from rice seeds, cowpea cystatin, and corn cystatin, showed that the avocado cystatin molecule has 60% and 54% residues identical with the two forms of the rice seed proteins, oryzacystatins I and II, respectively, and 64% and 63% with the cowpea and corn proteins, respectively. The totally conserved sequence, Gln-Val-Val-Ala-Gly, among several of the animal cystatins as well as phytocystatins, is at positions 47-51 in the avocado cystatin molecule.
Kimura
M
M
Laboratory of Biochemistry, Faculty of Agriculture, Kyushu University, Fukuoka, Japan.
Ikeda
T
T
Fukumoto
D
D
Yamasaki
N
N
Yonekura
M
M
D000595:Amino Acid Sequence; D015891:Cystatins / Q000032:analysis*; D015853:Cysteine Proteinase Inhibitors / Q000032:analysis*; D005638:Fruit / Q000737:chemistry*; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000032:analysis*; D017386:Sequence Homology, Amino Acid
D015891:Cystatins; D015853:Cysteine Proteinase Inhibitors; D010940:Plant Proteins