title: 113Cd nuclear magnetic resonance studies of cabbage histidinol dehydrogenase.
abstract: Histidinol dehydrogenase (HDH), a dimeric protein, catalyzes two sequential oxidation reactions to yield L-histidine from L-histidinol via L-histidinal. HDH contains 1 mol of Zn(II) per mol of subunit, and removal of this metal abolishes the enzymatic activity. On substitution of Zn(II) with 113Cd(II), the enzyme ([113Cd]HDH) showed similar catalytic activity. The 113Cd NMR spectra of [113Cd]HDH were measured under various conditions. The 113Cd NMR spectrum of [113Cd]HDH showed a resonance at 110 ppm, which indicates that the metal ion is bound to the protein by a combination of nitrogen and oxygen ligands. 113Cd NMR spectra of [113Cd]HDH were measured as complexes with two substrates (L-histidinol and DL-histidinal) and four inhibitors (imidazole, histamine, L-histidine, and DL-4-(4-imidazolyl)-3-amino-2-butanone) in the absence and presence of NAD+. Significant shifts of [113Cd]-HDH resonance in the presence of the ligand indicate that the metal ion is located in the catalytic site of HDH and that substrates and inhibitors interact with the metal ion. The role of the metal ion in the HDH reaction is discussed.
authors.affiliation: International Research Laboratories, Ciba-Geigy Japan Ltd., Takarazuka, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Kanaori
authors.forename: N
authors.initials: N
authors.lastname: Uodome
authors.forename: A
authors.initials: A
authors.lastname: Nagai
authors.forename: D
authors.initials: D
authors.lastname: Ohta
authors.forename: A
authors.initials: A
authors.lastname: Ogawa
authors.forename: G
authors.initials: G
authors.lastname: Iwasaki
authors.forename: A Y
authors.initials: AY
authors.lastname: Nosaka
mesh_terms: D000429:Alcohol Oxidoreductases / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000378:metabolism; D001937:Brassica / Q000201:enzymology*; D002104:Cadmium; D002384:Catalysis; D007554:Isotopes; D009682:Magnetic Resonance Spectroscopy; D009243:NAD / Q000737:chemistry; D013379:Substrate Specificity
chemical_list: D007554:Isotopes; D002104:Cadmium; D009243:NAD; D000429:Alcohol Oxidoreductases; C021358:histidinol dehydrogenase