Identification of 3 beta-hydroxy-5 alpha-cholest-6-ene-5-hydroperoxide in human oxidized LDL.
Oxidized LDL exhibits cytotoxic activity towards endothelial cells, which is thought to be mediated by the products derived also from cholesterol oxidation, mainly involving the B-ring. By LC-PB-EI-MS and EI-MS/MS analysis, we have identified 3 beta-hydroxy-5 alpha-cholest-6-ene-5-hydroperoxide among the cholesterol oxidation products in LDL incubated with micromolar concentrations of copper ions.
Caruso
D
D
Institute of Pharmacological Sciences, Milan, Italy.
Rasetti
M F
MF
De Angelis
L
L
Galli
G
G
D002784:Cholesterol / Q000031:analogs & derivatives* / Q000097:blood / Q000737:chemistry / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D003300:Copper; D006801:Humans; D015227:Lipid Peroxidation; D008077:Lipoproteins, LDL / Q000097:blood / Q000737:chemistry*; D013058:Mass Spectrometry; D015394:Molecular Structure; D010084:Oxidation-Reduction
D008077:Lipoproteins, LDL; D003300:Copper; C002843:cholesterol hydroperoxide; D002784:Cholesterol