The Hox-2.4 gene is not involved in the generation of IL-3 dependent multipotent FDCP-mix cell lines.
The establishment of IL-3-dependent multipotent progenitor cell lines from Hox-2.4-expressing bone marrow cells suggests that homeobox genes may contribute to immortalization of early myeloid cells. A survey of 20 independently derived multipotent IL-3-dependent cell lines established from either src-virus-infected long-term bone marrow cultures (FDCP-mix) or Multi-CSF-virus (M3MuV)-infected bone marrow revealed that Hox-2.4 was not expressed in any of these cell lines. In addition DNA rearrangements were not observed. We conclude that activation of Hox-2.4 is not an obligatory event in the immortalization of early myeloid cells.
Just
U
U
Department of Experimental Haematology, Paterson Institute for Cancer Research, Manchester, UK.
Kan
O
O
Fennelly
J
J
Dexter
T M
TM
Spooncer
E
E
D000818:Animals; D001854:Bone Marrow Cells; D002460:Cell Line; D015870:Gene Expression; D015321:Gene Rearrangement; D005801:Genes, Homeobox; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D018398:Homeodomain Proteins / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D007377:Interleukin-3 / Q000494:pharmacology*; D009052:Leukemia Virus, Murine; D051379:Mice; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics; D014157:Transcription Factors / Q000096:biosynthesis
D018398:Homeodomain Proteins; C095822:Hoxb8 protein, mouse; D007377:Interleukin-3; D009363:Neoplasm Proteins; D014157:Transcription Factors