Analysis of total lipid extracts from human liver by 13C and 1H nuclear magnetic resonance spectroscopy.
Lipid extracts of liver tissue from normal donors and from patients with acute liver failure were analyzed by 13C NMR and 1H NMR at 9.4 T. The spectra allowed estimation of (i) the free fatty acid to total fatty acid chains ratio, (ii) the polyunsaturated to monounsaturated lipids ratio, (iii) the glycerophospholipids to triacylglycerols ratio, (iv) the total cholesterol to total fatty acids molar ratio, (v) the acylcholesterol to cholesterol ratio, (vi) the phosphatidylcholine to phosphatidylethanolamine molar ratio, and (vii) the unsaturation ratio of the fatty acid chains. The values obtained for normal livers agree with literature data on lipid composition obtained by other techniques. The two pathologic livers differ from the normal in the composition of some lipids and show a higher free fatty acid to total fatty acid chains ratio and a lower polyunsaturated to monounsaturated lipid ratio, a lower acylcholesterol to cholesterol ratio, and a lower phosphatidylcholine to phosphatidylethanolamine ratio, whereas the ratio of total cholesterol to fatty acids and the unsaturation ratio remained unchanged. The detailed assignment of the 13C NMR spectra of lipid extracts from normal and pathologic human liver may contribute to direct future research programs on 13C NMR spatially localized spectroscopy of the liver in vivo.
Pollesello
P
P
Department of Medical Chemistry, University of Helsinki (Siltavuorenpenger 10), Helsinki, FIN-00014, Finland.
Eriksson
O
O
Hockerstedt
K
K
D002784:Cholesterol / Q000032:analysis; D005230:Fatty Acids, Nonesterified / Q000032:analysis; D006801:Humans; D008055:Lipids / Q000032:analysis*; D008099:Liver / Q000737:chemistry*; D008107:Liver Diseases / Q000175:diagnosis*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D010743:Phospholipids / Q000032:analysis
D005230:Fatty Acids, Nonesterified; D008055:Lipids; D010743:Phospholipids; D002784:Cholesterol