Solubility of artificial proteins with random sequences.
A library of artificial random proteins of 141 amino acid residues of which 95 are random and which includes the 20 kinds of amino acids was prepared. Out of the 25 identified random proteins, 5 were soluble in the cell lysate, indicating that about 20% of the random proteins expressed in Escherichia coli are expected to be soluble. The soluble random proteins RP3-42 and RP3-45 and insoluble RP3-70 were purified. The solubility of the purified form is the same as that in the cell lysate.
Prijambada
I D
ID
Department of Biotechnology, Faculty of Engineering, Osaka University, Japan.
Yomo
T
T
Tanaka
F
F
Kawama
T
T
Yamamoto
K
K
Hasegawa
A
A
Shima
Y
Y
Negoro
S
S
Urabe
I
I
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D001482:Base Composition; D001483:Base Sequence; D019143:Evolution, Molecular; D015723:Gene Library; D008969:Molecular Sequence Data; D011506:Proteins / Q000737:chemistry* / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000737:chemistry; D017422:Sequence Analysis, DNA; D012995:Solubility
D000596:Amino Acids; D011506:Proteins; D011993:Recombinant Fusion Proteins