Modulation of expression of class II MHC and CD40 molecules in murine B cells by various muramyl dipeptides.
Several compounds of the MDP (muramyl dipeptide) series which have the capacity to enhance the immune response to antigens exerted a comitogenic effect on murine splenic B cells. The expression of surface class II major histocompatibility and CD40 antigens was used to more accurately evaluate the comparative influence of the synthetic agents on mature B cells and on the pre-B cell line 70Z/3. MDP and two adjuvant-active analogs enhanced expression of both surface molecules and increased the response to lipopolysaccharide (LPS) or interferon-gamma (IFN-gamma) in splenic B cells. The three synthetic adjuvants alone did not lead to expression of cell-surface I-Ad or CD40 in 70Z/3 cells, indicating that they were unable by themselves to achieve differentiation of pre-B cells to a mature B cell phenotype. However, they increased the CD40 level induced by treatment with LPS. In this cell line, the response (CD40 protein and mRNA) to IFN-gamma was strongly increased by MDP but not by the two other compounds. Actually, MDP was the only adjuvant among the three compounds to functionally activate the transcription factor NF-kappaB, to induce kappa transcription, and to stimulate surface kappa light-chain expression in 70Z/3 cells. The response to muramyl dipeptides in mature splenic B cells could appear independent of the transcription factor.
Cohen
L Y
LY
Laboratoire d'Immunopharmacologie, CNRS URA, Institut des Cordeliers, Paris, France.
Bahr
G M
GM
Darcissac
E C
EC
Parant
M A
MA
D000119:Acetylmuramyl-Alanyl-Isoglutamine / Q000494:pharmacology*; D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D001483:Base Sequence; D019013:CD40 Antigens / Q000096:biosynthesis*; D002478:Cells, Cultured; D005260:Female; D005434:Flow Cytometry; D000949:Histocompatibility Antigens Class II / Q000096:biosynthesis*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data
D000276:Adjuvants, Immunologic; D019013:CD40 Antigens; D000949:Histocompatibility Antigens Class II; D000119:Acetylmuramyl-Alanyl-Isoglutamine