Regulation of olfactory signalling via cGMP-dependent protein kinase.
Strong odor stimuli elicit a slow and sustained increase of the cGMP concentration in isolated rat olfactory cilia. Elevated cGMP levels appear to attenuate the primary response to odorant stimulation. Incubating cilia with membrane-permeable cGMP derivates caused a significantly reduced cAMP signal in response to odorant stimulation. This inhibitory effect was mimicked by 8-(4-chlorophenlythio)-cGMP, a selective activator of cGMP-activated protein kinases; in contrast, a selective inhibitor, [8-(4-chlorophenylthio)-guanosine-3',5'-cyclic monophosphorothioate] of cGMP kinases enhanced the reactivity to odorant stimulation. The data suggest that the responsiveness of olfactory sensory cells is governed by a cGMP-dependent protein kinase. Western-blot analysis using subtype-specific antibodies indicated that cytosolic type-I cGMP kinase, but not the membrane-associated type-II cGMP kinase, is expressed in olfactory sensory neurons.
Kroner
C
C
University Stuttgart-Hohenheim, Institute of Zoophysiology, Germany.
Boekhoff
I
I
Lohmann
S M
SM
Genieser
H G
HG
Breer
H
H
D000818:Animals; D002628:Chemoreceptor Cells / Q000502:physiology*; D000242:Cyclic AMP / Q000378:metabolism; D017869:Cyclic GMP-Dependent Protein Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D009831:Olfactory Mucosa / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015290:Second Messenger Systems; D015398:Signal Transduction; D012903:Smell / Q000502:physiology*
D000242:Cyclic AMP; D017869:Cyclic GMP-Dependent Protein Kinases