title: Protective Role of TNF-alpha in respiratory syncytial virus infection in vitro and in vivo.
abstract: Respiratory syncytial virus (RSV) infection causes substantial morbidity in young children and immunocompromised adults, yet its pathogenesis is poorly understood. Because the proinflammatory cytokine tumor necrosis factor-alpha (TNF-alpha) may be important in host response to viral infection, HEp-2 cells were treated with TNF-alpha and mice were given TNF-alpha antibody before RSV infection. Pretreatment of HEp-2 cells with TNF-alpha inhibited RSV replication as determined by cytopathic effect. Respiratory syncytial virus-infected BALB/c mice treated with antibody to TNF-alpha had greater maximal weight loss and slower recovery time than control mice. These results suggest a protective role for TNF-alpha in RSV infection.
authors.affiliation: Departmemt of Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee 37232-2605, USA.
authors.forename: K M
authors.initials: KM
authors.lastname: Neuzil
authors.forename: Y W
authors.initials: YW
authors.lastname: Tang
authors.forename: B S
authors.initials: BS
authors.lastname: Graham
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology; D001835:Body Weight; D006528:Carcinoma, Hepatocellular; D003588:Cytopathogenic Effect, Viral; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D018357:Respiratory Syncytial Virus Infections / Q000503:physiopathology*; D012136:Respiratory Syncytial Viruses / Q000187:drug effects / Q000502:physiology*; D013047:Specific Pathogen-Free Organisms; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology / Q000494:pharmacology / Q000502:physiology*; D010948:Viral Plaque Assay; D014779:Virus Replication
chemical_list: D000906:Antibodies; D014409:Tumor Necrosis Factor-alpha