The apparent coupling between synthesis and posttranslational modification of Escherichia coli acyl carrier protein is due to inhibition of amino acid biosynthesis.
Acyl carrier protein (ACP) is modified on serine 36 by the covalent posttranslational attachment of 4'-phosphopantetheine from coenzyme A (CoA), and this modification is required for lipid biosynthesis. Jackowski and Rock (J. Biol. Chem 258:15186-15191, 1983) reported that upon depletion of the CoA pool by starvation for a CoA precursor, no accumulation of the unmodified form of ACP (apo-ACP) was detected. We report that this lack of apo-ACP accumulation results from decreased translation of the acpP mRNAs because of the limitation of the synthesis of glutamate and other amino acids made directly from tricarboxylic acid cycle intermediates.
Keating
D H
DH
Department of Microbiology, University of Illinois, Urbana 61801, USA.
Zhang
Y
Y
Cronan
J E
JE
D000213:Acyl Carrier Protein / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism*; D000596:Amino Acids / Q000096:biosynthesis*; D001059:Apoproteins / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism*; D003065:Coenzyme A / Q000502:physiology; D004926:Escherichia coli / Q000378:metabolism; D029968:Escherichia coli Proteins; D054889:Fatty Acid Synthase, Type II; D005798:Genes, Bacterial; D010442:Peptide Chain Initiation, Translational; D011401:Promoter Regions, Genetic; D011499:Protein Processing, Post-Translational / Q000502:physiology*; D012329:RNA, Bacterial / Q000096:biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D014158:Transcription, Genetic; D001616:beta-Galactosidase / Q000378:metabolism
D000213:Acyl Carrier Protein; D000596:Amino Acids; D001059:Apoproteins; D029968:Escherichia coli Proteins; D012329:RNA, Bacterial; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; C040157:acpP protein, E coli; D001616:beta-Galactosidase; D054889:Fatty Acid Synthase, Type II; D003065:Coenzyme A