Cloning and sequencing of a human cDNA encoding a putative transcription factor containing a bromodomain.
A 2985 bp cDNA was isolated from a Lambda Zap Express library and sequenced. The cDNA appeared to represent a previously unknown gene that encodes and acidic 757 amino acid protein containing a bromodomain, several potential sites for phosphorylation by casein kinase-II and small proline-rich segments. The results suggest that the encoded protein might be a novel transcription factor.
Nielsen
M S
MS
Department of Medical Biochemistry, University of Aarhus, Denmark. msn@biobase.dk
Petersen
C M
CM
Gliemann
J
J
Madsen
P
P
D000595:Amino Acid Sequence; D001483:Base Sequence; D047390:Casein Kinase II; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D015723:Gene Library; D005796:Genes; D006801:Humans; D015459:Leukemia-Lymphoma, Adult T-Cell / Q000235:genetics / Q000473:pathology; D008969:Molecular Sequence Data; D010766:Phosphorylation; D011499:Protein Processing, Post-Translational; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D017434:Protein Structure, Tertiary; D011988:Receptors, Thyroid Hormone; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000235:genetics*; D014407:Tumor Cells, Cultured; D000092468:Bromodomain Containing Proteins
D047390:Casein Kinase II; D018076:DNA, Complementary; D017346:Protein Serine-Threonine Kinases; D011988:Receptors, Thyroid Hormone; D014157:Transcription Factors; C099139:BRD8 protein, human; D000092468:Bromodomain Containing Proteins