Characterization of representative enzymes from a sulfate reducing bacterium implicated in the corrosion of steel.
This communication reports the isolation, purification and characterization of key enzymes involved in dissimilatory sulfate reduction of a sulfate reducing bacterium classified as Desulfovibrio desulfuricans subspecies desulfuricans New Jersey (NCIMB 8313) (Ddd NJ). The chosen strain, originally recovered from a corroding cast iron heat exchanger, was grown in large scale batch cultures. Physico-chemical and spectroscopic studies of the purified enzymes were carried out. These analyses revealed a high degree of similarity between proteins isolated from the DddNJ strain and the homologous proteins obtained from Desulfomicrobium baculatus Norway 4. In view of the results obtained, taxonomic reclassification of Desulfovibrio desulfuricans subspecies desulfuricans New Jersey (NCIMB 8313) into Desulfomicrobium baculatus (New Jersey) is proposed.
Pereira
A S
AS
Departmento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Portugal.
Franco
R
R
Feio
M J
MJ
Pinto
C
C
Lampreia
J
J
Reis
M A
MA
Calvete
J
J
Moura
I
I
Beech
I
I
Lino
A R
AR
Moura
J J
JJ
D003343:Corrosion; D003574:Cytochrome c Group / Q000378:metabolism*; D003901:Desulfovibrio / Q000201:enzymology*; D004578:Electron Spin Resonance Spectroscopy; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000378:metabolism*; D050862:Oxidoreductases Acting on Sulfur Group Donors / Q000378:metabolism*; D013232:Steel / Q000737:chemistry*
D003574:Cytochrome c Group; D013232:Steel; C017165:cytochrome c(3); D010088:Oxidoreductases; C019981:cytochrome c3 hydrogenase; D050862:Oxidoreductases Acting on Sulfur Group Donors