Protective activity of a murine monoclonal antibody against acute and chronic experimental infection with type IV group B streptococcus.
A murine IgM monoclonal antibody (MAb H11) was developed against the type polysaccharide capsular antigen of group B streptococcus (GBS), serotype IV, after intraperitoneal immunisation of BALB/c mice with heat-killed bacteria. MAb H11 reacted in immunodiffusion with the purified polysaccharide in both its sialylated and desialylated form, giving a line of identity, and opsonised type IV GBS strains in an in vitro assay. When administered at the time of intraperitoneal lethal challenge with homologous GBS, or 4 h earlier, MAb H11 protected 90% of the mice. Protection was still observed when MAb H11 was given 4 h after the challenge. This MAb was strongly effective in preventing septic arthritis induced by type IV GBS.
Ricci
M L
ML
Laboratory of Bacteriology and Medical Mycology, Istituto Superiore di Sanità, Rome, Italy.
von Hunolstein
C
C
Gomez
M J
MJ
Parisi
L
L
Tissi
L
L
Orefici
G
G
D000208:Acute Disease; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology* / Q000627:therapeutic use; D001170:Arthritis, Infectious / Q000517:prevention & control; D016667:Bacterial Capsules / Q000276:immunology; D002908:Chronic Disease; D005260:Female; D006825:Hybridomas; D005779:Immunodiffusion; D007075:Immunoglobulin M / Q000096:biosynthesis / Q000276:immunology / Q000627:therapeutic use; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010587:Phagocytosis; D011135:Polysaccharides, Bacterial / Q000276:immunology; D013290:Streptococcal Infections / Q000517:prevention & control*; D013292:Streptococcus agalactiae / Q000276:immunology*
D000911:Antibodies, Monoclonal; D007075:Immunoglobulin M; D011135:Polysaccharides, Bacterial