Change in membrane permeability induced by protegrin 1: implication of disulphide bridges for pore formation.
Protegrin 1 (PG-1) is a naturally occurring cationic antimicrobial peptide that is 18 residues long, has an aminated carboxy terminus and contains two disulphide bridges. Here, we investigated the antimicrobial activity of PG-1 and three linear analogues. Then, the membrane permeabilisation induced by these peptides was studied upon Xenopus laevis oocytes by electrophysiological methods. From the results obtained, we concluded that protegrin is able to form anion channels. Moreover, it seems clear that the presence of disulphide bridges is a prerequisite for the pore formation at the membrane level and not for the antimicrobial activity.
Mangoni
M E
ME
Centre de Recherches de Biochimie Macromoléculaire, CNRS-INSERM, Montpellier, France.
Aumelas
A
A
Charnet
P
P
Roumestand
C
C
Chiche
L
L
Despaux
E
E
Grassy
G
G
Calas
B
B
Chavanieu
A
A
D000595:Amino Acid Sequence; D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology; D023181:Antimicrobial Cationic Peptides; D001798:Blood Proteins / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D002463:Cell Membrane Permeability / Q000187:drug effects*; D023082:Defensins; D004220:Disulfides / Q000737:chemistry / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects; D007473:Ion Channels / Q000187:drug effects / Q000378:metabolism; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D009865:Oocytes; D018408:Patch-Clamp Techniques; D010455:Peptides / Q000737:chemistry / Q000494:pharmacology; D011506:Proteins / Q000737:chemistry / Q000494:pharmacology*; D016415:Sequence Alignment; D013210:Staphylococcus / Q000187:drug effects; D013329:Structure-Activity Relationship; D014982:Xenopus laevis
D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides; D001798:Blood Proteins; D023082:Defensins; D004220:Disulfides; D007473:Ion Channels; D010455:Peptides; D011506:Proteins; C094880:protegrin-1; D002118:Calcium