Changes in permeability of sheep's lungs by oleic acid are dose dependent.
OBJECTIVE
To evaluate a two-isotope technique to detect graded changes in pulmonary microvascular permeability.


METHODS
Open experimental study.


METHODS
University hospital, Sweden.


METHODS
Fifty-seven sheep.


METHODS
Catherisation of one carotid artery, pulmonary artery and central vein. Control group 1 (n = 10), control group 2 (n = 12) had an additional pulmonary artery balloon catheter inserted, experimental group 3 (n = 9) was given oleic acid 0.005 mg kg-1 BW, experimental group 4 (n = 12) received oleic acid 0.02 mg kg-1 BW and experimental group 5 (n = 11) 0.05 mg kg-1 BW. Groups 3-5 had all PA catheters. All animals were intubated and ventilated artificially. Duration of experiments was 6 hours.


METHODS
Transferrin was labelled in vivo with 113mIn chloride and erythrocytes with 99mTc following injection of stannous chloride. External gamma counting was corrected for background, decay and scatter. Blood activity was used as reference. Normalised slope index (NSI) and transferrin leak index (TLI) were calculated as measures of pulmonary microvascular permeability.


RESULTS
A graded response in both NSI and TLI was found. Insertion of the PA catheter (group 2) significantly increased NSI from (group 1) (1.4 (0.1)) 10(-4) min-1 to (11 (2)) 10(-4) min-1 (p < 0.05). TLI increased significantly from (9 (2)) 10(-4) min-1 to (72 (13)) 10(-4) min-1. Oleic acid increased NSI significantly to (13 (1)) 10(-4) min-1, (32 (2)) 10(-4) min-1 and (61 (5)) 10(-4) min-1 in groups 3-5, respectively. Corresponding values for TLI were (95 (13)) 10(-4) min-1, (162 (6)) 10(-4) min-1 and (228 (26)) 10-4 min-1, respectively.


CONCLUSIONS
The double-isotope technique of external monitoring of permeability changes to protein in the lungs was sensitive to pick up graded increments in leakage, related in a dose-dependent way to lung injury.
Bergdahl
S
S
Department of Surgery, Ostra Hospital, Göteborg University, Sweden.
Larsson
A
A
Smith
L
L
Alpsten
M
M
Risberg
B
B
D000818:Animals; D002199:Capillary Permeability / Q000187:drug effects / Q000502:physiology*; D004305:Dose-Response Relationship, Drug; D004912:Erythrocytes; D006439:Hemodynamics; D007205:Indium Radioisotopes; D007553:Isotope Labeling / Q000379:methods*; D008168:Lung / Q000098:blood supply* / Q000187:drug effects; D019301:Oleic Acid; D009829:Oleic Acids / Q000633:toxicity*; D011652:Pulmonary Circulation / Q000502:physiology; D011654:Pulmonary Edema / Q000139:chemically induced / Q000503:physiopathology*; D012680:Sensitivity and Specificity; D012756:Sheep; D013667:Technetium
D007205:Indium Radioisotopes; D009829:Oleic Acids; D019301:Oleic Acid; D013667:Technetium