Characterization of monoclonal antibodies specific for the human growth hormone 22K and 20K isoforms.
We have derived and characterized a set of monoclonal antibodies (mAb) specific for the different human GH (hGH) isoforms. The binding characteristics of each antibody to the hGH isoforms (22K and 20K) were analyzed in direct and competitive immunoassays as well as by Western blot. We studied the effects of these mAb on the biological activity of hGH and showed that they specifically block their respective activities. Using these mAb, we developed several immunoassays that have been applied for the quantitation of the different hGH isoforms in body fluids. Therefore, these mAb may help to unravel the biological function of these variants.
Mellado
M
M
Department of Immunology and Oncology, Centro Nacional de Biotecnologia, Campus Cantoblanco, Universidad Autonoma, Madrid, Spain. jmmelladosamba.cnb.uam.es.
Rodriguez-Frade
J M
JM
Kremer
L
L
Martinez-Alonso
C
C
D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D015153:Blotting, Western / Q000379:methods; D001826:Body Fluids / Q000737:chemistry; D013006:Growth Hormone / Q000032:analysis* / Q000276:immunology; D006801:Humans; D007124:Immunoenzyme Techniques; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D009124:Muscle Proteins / Q000096:biosynthesis*; D062085:RNA, Long Noncoding; D022661:RNA, Untranslated; D011863:Radioimmunoassay / Q000379:methods; D011994:Recombinant Proteins / Q000032:analysis / Q000276:immunology; D012680:Sensitivity and Specificity
D000911:Antibodies, Monoclonal; C094335:H19 long non-coding RNA; D009124:Muscle Proteins; D062085:RNA, Long Noncoding; D022661:RNA, Untranslated; D011994:Recombinant Proteins; D013006:Growth Hormone