The ganglioside GM1 enhances microtubule networks and changes the morphology of Neuro-2a cells in vitro by altering the distribution of MAP2.
The effect of ganglioside GM1 on components of the neuronal cytoskeleton was studied in Neuro-2a neuroblastoma cells using immunofluorescent, immunogold-labeled, and Western-blot analysis. Exposure of cells to GM1 for 24 h resulted in an increased microtubular network and level of tubulin, a redistribution of MAP2 immunoreactivity from perikarya to distal neuritic processes, and an increased MAP2 gold label in the subplasmalemmal cytoplasm, neuritic spines, and growth cones. A similar change in the distribution of actin-positive fluorescent immunoreactivity was observed. In contrast to the redistribution of MAP2, immunolocalization of MAP5 and tau did not change following 24 h GM1 exposure. Our results suggest that gangliosides enhance neuritogenesis by selectively altering the distribution of MAP2 from perikaryon to neuritic spines. Furthermore, the enhanced presence of MAP2 in regions known to be rich in microfilaments following GM1 treatment suggests that an interaction of MAP2 with microfilaments may be necessary for early neurite formation.
Wang
L J
LJ
Department of Anatomical Sciences and Neurobiology, University of Louisville School of Medicine, Kentucky 40292, USA.
Colella
R
R
Yorke
G
G
Roisen
F J
FJ
D000199:Actins / Q000032:analysis; D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D005455:Fluorescent Antibody Technique; D005677:G(M1) Ganglioside / Q000494:pharmacology*; D051379:Mice; D016253:Microscopy, Immunoelectron; D008869:Microtubule-Associated Proteins / Q000032:analysis / Q000187:drug effects*; D008870:Microtubules / Q000187:drug effects*; D008969:Molecular Sequence Data; D009447:Neuroblastoma; D009474:Neurons / Q000737:chemistry / Q000166:cytology / Q000648:ultrastructure; D014407:Tumor Cells, Cultured / Q000737:chemistry
D000199:Actins; D008869:Microtubule-Associated Proteins; D005677:G(M1) Ganglioside