Characterization of long-term cultures of hepatitis C virus.
The human T- and B-cell lines HPBMa10-2 and Daudi produced infectious hepatitis C virus (HCV) for more than 1 year after infection. The infectivity titer of the cell culture-grown HCV and its genome titer were comparable. The virion density in sucrose was around 1.12 g/ml. Among the 13 variants detected in the inoculum, 7 were adsorbed by the cells and one particular HCV sequence which was present in minor quantities in the inoculum persisted.
Nakajima
N
N
Department of Bacteriology, Faculty of Medicine, University of Tokyo, Japan.
Hijikata
M
M
Yoshikura
H
H
Shimizu
Y K
YK
D000818:Animals; D001402:B-Lymphocytes; D001483:Base Sequence; D002460:Cell Line; D014644:Genetic Variation; D016679:Genome, Viral; D016174:Hepacivirus / Q000235:genetics / Q000472:pathogenicity* / Q000502:physiology*; D006801:Humans; D008969:Molecular Sequence Data; D002679:Pan troglodytes; D016133:Polymerase Chain Reaction; D013601:T-Lymphocytes; D013997:Time Factors; D014407:Tumor Cells, Cultured; D014771:Virion / Q000502:physiology