Sequence variation of latent membrane protein-1 of Epstein-Barr virus strains associated with hairy leukoplakia.
The Epstein-Barr virus (EBV) latent membrane protein (LMP)-1 is expressed in hairy leukoplakia (HL), but data on LMP-1 sequence variation of HL isolates are limited. Variation in the LMP-1 repeat region and presence of a 30-nt deletion were studied using DNA scrapings from 28 HL lesions. cDNAs from 3 different HL isolates were sequenced, 2 from lymphocyte cell lines (LCLs) generated using HL biopsy material. The deletion was found in 16 (57%) of 28 HL scraping, and multiple repeat region variants were seen in 13 scrapings (46%). HL LMP sequence were similar to those described in nasopharyngeal cancer and lymphoma tissues, including two motifs of four amino acid changes relative to B95-8 upstream and downstream of the repeat region, respectively. Generation of LCLs using HL biopsy material confirmed the ability of HL EBV strains to infect and transform lymphocytes.
Palefsky
J M
JM
Department of Stomatology, University of California, San Francisco, 94143-0100, USA.
Berline
J
J
Penaranda
M E
ME
Lennette
E T
ET
Greenspan
D
D
Greenspan
J S
JS
D000595:Amino Acid Sequence; D002460:Cell Line; D002472:Cell Transformation, Viral; D014644:Genetic Variation; D004854:Herpesvirus 4, Human / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D006801:Humans; D017733:Leukoplakia, Hairy / Q000209:etiology / Q000821:virology*; D008214:Lymphocytes; D008969:Molecular Sequence Data; D017384:Sequence Deletion; D014763:Viral Matrix Proteins / Q000235:genetics*
C037662:EBV-associated membrane antigen, Epstein-Barr virus; D014763:Viral Matrix Proteins