title: Alkylation of cellulosic membranes results in reduced complement activation.
abstract: 4-Vinyl pyridine was grafted to the surface of the cellulosic membrane Cuprophan, and subsequently alkylated with both C10 and C16 aliphatic chains. Complement activation of heparinized human blood, corrected for anaphylatoxin adhesion, was measured by radioimmunoassay. The surface treatments both yielded substantial reductions in C5a activity, with a lessor reduction in C3a and C4a activity. Alkylation with 10 and 16 carbon chains resulted both in enhancements of albumin adsorption and stability. These enhancements as well as the reductions in complement activation were statistically indistinguishable between the two treatments. The reduction in complement activation was influenced more by adsorption of endogenous albumin and possibly by the vinyl pyridine graft, than the removal of surface active hydroxyl groups from Cuprophan.
authors.affiliation: Carbomedics, Austin, TX 78752-1793, USA.
authors.forename: J R
authors.initials: JR
authors.lastname: Frautschi
authors.forename: R C
authors.initials: RC
authors.lastname: Eberhart
authors.forename: J A
authors.initials: JA
authors.lastname: Hubbell
authors.forename: B D
authors.initials: BD
authors.lastname: Clark
authors.forename: J A
authors.initials: JA
authors.lastname: Gelfand
mesh_terms: D000327:Adsorption; D000478:Alkylation; D000704:Analysis of Variance; D001672:Biocompatible Materials; D002482:Cellulose / Q000031:analogs & derivatives*; D003167:Complement Activation / Q000187:drug effects; D015926:Complement C3a / Q000187:drug effects / Q000378:metabolism; D015934:Complement C4a / Q000187:drug effects / Q000378:metabolism; D015936:Complement C5a / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D008567:Membranes, Artificial; D011725:Pyridines / Q000494:pharmacology*; D012709:Serum Albumin / Q000378:metabolism; D013329:Structure-Activity Relationship; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D008567:Membranes, Artificial; D011725:Pyridines; D012709:Serum Albumin; D015926:Complement C3a; D015934:Complement C4a; D015936:Complement C5a; D002482:Cellulose; C009865:cuprammonium cellulose; C029351:4-vinylpyridine