Filtration of human seminal specimens using LeukoSorb leukocyte-retention medium.
OBJECTIVE
To compare LeukoSorb filtration with Percoll centrifugation in preparing sperm.


METHODS
Each of 26 human seminal specimens were analyzed before and after two preparation methods.


RESULTS
In normospermic specimens (n = 11), LeukoSorb produced higher results than Percoll did with regard to mean percentage of motility (72.7% vs 59.2%), total swollen sperm in a hypo-osmotic swelling test (84.8% vs 59.0%), (g)-type swollen sperm (37.8% vs 23.4%) and tail abnormality (20.4% vs 10.1%). In asthenospermic specimens (n = 15), LeukoSorb produced higher results than Percoll did with regard to mean percentage of motile sperm recovery (22.2% vs 10.1%), motility (46.9% vs 23.2%), viability (75.6% vs 54.7%), total swollen sperm (84.8% vs 59.0%) and tail abnormality (42.2% vs 24.3%), although the mean percentage of head abnormality was lower with LeukoSorb than with Percoll (30.6% vs 40.2%) vs 40.2%). In pyospermic specimens (n = 10), the mean concentration of round cells after LeukoSorb decreased significantly more than with Percoll (p = 0.029). The filtration time correlated with the total sperm concentration.


CONCLUSIONS
LeukoSorb filtration is useful for preparing asthenospermic and pyospermic seminal specimens.
Fujii
S
S
Department of Obstetrics and Gynecology, Hirosaki University School of Medicine, Japan.
Sagara
M
M
Ikeda
S
S
Kagiya
A
A
Sato
S
S
Saito
Y
Y
D002498:Centrifugation; D005374:Filtration / Q000379:methods*; D006801:Humans; D008297:Male; D013048:Specimen Handling / Q000379:methods*; D013076:Sperm Count; D013081:Sperm Motility; D013094:Spermatozoa; D013997:Time Factors