title: Endoglucanase II (EGII) of Penicillium janthinellum: cDNA sequence, heterologous expression and promotor analysis.
abstract: The cDNA coding for the endoglucanase EGII of P. janthinellum was cloned and sequenced. The open reading frame comprises 1230 nucleotides and the deduced amino-acid sequence shows an overall homology of 63% with the T. reesei egl2. The cellulose-binding domain of EGII represents a typical member of the A family of cellulases. The egl2 gene is only induced by cellulose or cellobiose and not by sophorose. A promotor fragment including 1 kb was cloned and sequenced. Three major transcription startpoints were identified. Five motifs matching the binding site of the carbon-catabolite repressor CREA of A. nidulans were detected. Their potential implication in repression was analyzed by bandshift assays.
authors.affiliation: AG Mikrobiologie, Institut für Biologie, Humboldt-Universität zu Berlin, D-14532 Kleinmachnow, Germany.
authors.forename: G
authors.initials: G
authors.lastname: Mernitz
authors.forename: A
authors.initials: A
authors.lastname: Koch
authors.forename: B
authors.initials: B
authors.lastname: Henrissat
authors.forename: G
authors.initials: G
authors.lastname: Schulz
mesh_terms: D001483:Base Sequence; D015152:Blotting, Northern; D002480:Cellulase / Q000096:biosynthesis / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D010407:Penicillium / Q000201:enzymology / Q000235:genetics*; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000096:biosynthesis; D012441:Saccharomyces cerevisiae / Q000235:genetics; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D013379:Substrate Specificity
chemical_list: D018076:DNA, Complementary; D011994:Recombinant Proteins; C056373:endoglucanase 2; D002480:Cellulase