Production and crystallization of MHC class I B allele single peptide complexes.
Major histocompatibility complex class I B alleles, HLA B8, B53 and B3501 have been cloned, expressed, refolded and crystallized in specific complexes with a number of different 8-mer and 9-mer peptides. For some of these crystallization was initiated by cross-seeding between different B allele complexes. All crystallize in the space group P212121, with similar unit cell dimensions of approximately 52 A X 81 A X 112 A, contain one complex per asymmetric unit and diffract to approximately 2.0 A resolution.
Reid
S W
SW
Laboratory of Molecular Biophysics, Oxford, UK.
Smith
K J
KJ
Jakobsen
B K
BK
O'Callaghan
C A
CA
Reyburn
H
H
Harlos
K
K
Stuart
D I
DI
McMichael
A J
AJ
Bell
J I
JI
Jones
E Y
EY
D000483:Alleles; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D017931:DNA Primers; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000737:chemistry; D004926:Escherichia coli / Q000235:genetics; D005805:Genes, MHC Class I / Q000235:genetics*; D006680:HLA Antigens / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D015795:HLA-B8 Antigen / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000737:chemistry / Q000378:metabolism*; D011487:Protein Conformation; D017510:Protein Folding; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism
D017931:DNA Primers; D000939:Epitopes; D006680:HLA Antigens; C078438:HLA-B53 antigen; D015795:HLA-B8 Antigen; D009842:Oligopeptides; D011994:Recombinant Proteins