title: Differential regulation of GPI-linked molecules on leukaemic promyelocytes treated in vitro with all-trans retinoic acid.
abstract: It has been demonstrated that certain cell-surface proteins are anchored to the cell membrane by a unique structure known as the glycosylphosphatidylinositol (GPI) anchor whose absence has been reported on blood cells from patients with paroxysmal nocturnal haemoglobinuria. We have investigated the expression of CD16/Fc(tau)R-III and CD66b GPI linked molecules at the surface of blast cells from five acute promyelocytic leukaemia (APL) patients before and after in vitro stimulation with all-trans retinoic acid (ATRA). We observed that whereas CD66b antigen exhibited a strong ATRA-driven up-regulation in all cases studied, CD16 expression was unaffected by the treatment with the drug.
authors.affiliation: Divisione di Oncologia Sperimentale C, Immunologia, Istituto Nazionale dei Tumori, Fondazione G. Pascale, Naples, Italy.
authors.forename: R
authors.initials: R
authors.lastname: Di Noto
authors.forename: E M
authors.initials: EM
authors.lastname: Schiavone
authors.forename: C
authors.initials: C
authors.lastname: Lo Pardo
authors.forename: F
authors.initials: F
authors.lastname: Ferrara
authors.forename: C
authors.initials: C
authors.lastname: Manzo
authors.forename: C
authors.initials: C
authors.lastname: Vacca
authors.forename: L
authors.initials: L
authors.lastname: Del Vecchio
mesh_terms: D015703:Antigens, CD; D000951:Antigens, Neoplasm; D015815:Cell Adhesion Molecules; D058851:GPI-Linked Proteins; D017261:Glycosylphosphatidylinositols / Q000378:metabolism; D006801:Humans; D015473:Leukemia, Promyelocytic, Acute / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000378:metabolism; D017452:Receptors, IgG / Q000378:metabolism; D014212:Tretinoin / Q000494:pharmacology*; D015854:Up-Regulation
chemical_list: D015703:Antigens, CD; D000951:Antigens, Neoplasm; C504120:CEACAM8 protein, human; D015815:Cell Adhesion Molecules; D058851:GPI-Linked Proteins; D017261:Glycosylphosphatidylinositols; D008562:Membrane Glycoproteins; D017452:Receptors, IgG; D014212:Tretinoin