title: Sequence variation of latent membrane protein-1 of Epstein-Barr virus strains associated with hairy leukoplakia.
abstract: The Epstein-Barr virus (EBV) latent membrane protein (LMP)-1 is expressed in hairy leukoplakia (HL), but data on LMP-1 sequence variation of HL isolates are limited. Variation in the LMP-1 repeat region and presence of a 30-nt deletion were studied using DNA scrapings from 28 HL lesions. cDNAs from 3 different HL isolates were sequenced, 2 from lymphocyte cell lines (LCLs) generated using HL biopsy material. The deletion was found in 16 (57%) of 28 HL scraping, and multiple repeat region variants were seen in 13 scrapings (46%). HL LMP sequence were similar to those described in nasopharyngeal cancer and lymphoma tissues, including two motifs of four amino acid changes relative to B95-8 upstream and downstream of the repeat region, respectively. Generation of LCLs using HL biopsy material confirmed the ability of HL EBV strains to infect and transform lymphocytes.
authors.affiliation: Department of Stomatology, University of California, San Francisco, 94143-0100, USA.
authors.forename: J M
authors.initials: JM
authors.lastname: Palefsky
authors.forename: J
authors.initials: J
authors.lastname: Berline
authors.forename: M E
authors.initials: ME
authors.lastname: Penaranda
authors.forename: E T
authors.initials: ET
authors.lastname: Lennette
authors.forename: D
authors.initials: D
authors.lastname: Greenspan
authors.forename: J S
authors.initials: JS
authors.lastname: Greenspan
mesh_terms: D000595:Amino Acid Sequence; D002460:Cell Line; D002472:Cell Transformation, Viral; D014644:Genetic Variation; D004854:Herpesvirus 4, Human / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D006801:Humans; D017733:Leukoplakia, Hairy / Q000209:etiology / Q000821:virology*; D008214:Lymphocytes; D008969:Molecular Sequence Data; D017384:Sequence Deletion; D014763:Viral Matrix Proteins / Q000235:genetics*
chemical_list: C037662:EBV-associated membrane antigen, Epstein-Barr virus; D014763:Viral Matrix Proteins