title: Characterization of two class II chitinase genes from peanut and expression studies in transgenic tobacco plants.
abstract: Two different genes encoding class II chitinases from peanut (Arachis hypogaea L. cv. NC4), A.h.Chi2;1 and A.h.Chi2;2, have been cloned. In peanut cell suspension cultures, mRNA levels of A.h.Chi2;2 increased after ethylene or salicylate treatment and in the presence of conidia from Botrytis cinerea. The second gene, A.h.Chi2;1, was only expressed after treatment with the fungal spores. Transgenic tobacco plants containing the complete peanut A.h.Chi2;1 gene exhibited essentially the same expression pattern in leaves as observed in peanut cell cultures. Expression characteristics of transgenic tobacco carrying a promoter-GUS fusion of A.h.Chi2;1 are described.
authors.affiliation: Max-Planck-Institut für Züchtungsforschung, Köln, FRG.
authors.forename: J W
authors.initials: JW
authors.lastname: Kellmann
authors.forename: T
authors.initials: T
authors.lastname: Kleinow
authors.forename: K
authors.initials: K
authors.lastname: Engelhardt
authors.forename: C
authors.initials: C
authors.lastname: Philipp
authors.forename: D
authors.initials: D
authors.lastname: Wegener
authors.forename: J
authors.initials: J
authors.lastname: Schell
authors.forename: P H
authors.initials: PH
authors.lastname: Schreier
mesh_terms: D000595:Amino Acid Sequence; D010367:Arachis / Q000201:enzymology / Q000235:genetics* / Q000382:microbiology; D001483:Base Sequence; D002688:Chitinases / Q000096:biosynthesis / Q000145:classification / Q000235:genetics*; D005030:Ethylenes / Q000494:pharmacology; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D017930:Genes, Reporter; D015698:Genomic Library; D003904:Mitosporic Fungi / Q000472:pathogenicity; D008969:Molecular Sequence Data; D010937:Plant Growth Regulators / Q000494:pharmacology; D010940:Plant Proteins / Q000096:biosynthesis / Q000145:classification / Q000235:genetics*; D030821:Plants, Genetically Modified; D010947:Plants, Toxic; D011994:Recombinant Proteins / Q000096:biosynthesis; D012459:Salicylates / Q000494:pharmacology; D020156:Salicylic Acid; D017386:Sequence Homology, Amino Acid; D014026:Nicotiana / Q000235:genetics
chemical_list: D005030:Ethylenes; D010937:Plant Growth Regulators; D010940:Plant Proteins; D011994:Recombinant Proteins; D012459:Salicylates; C053376:pathogenesis-related proteins, plant; C036216:ethylene; C099227:CHI2,2 protein, Arachis hypogaea; D002688:Chitinases; C099226:chi2-1 protein, Arachis hypogaea; D020156:Salicylic Acid