Growth suppression of a cervical cancer cell line (TMCC-1) by the human wild-type p53 gene.
To investigate the effects of human wild-type p53 expression on the proliferation of cervical carcinoma cells, a plasmid, pMO7-hp53, which contains a full-length cDNA of the human wild-type p53 (wt-p53) gene, was transfected into a cell line (TMCC-1) derived from an endocervical type, human papilloma virus-positive adenocarcinoma of the uterine cervix. The exogenous wt-p53 expression induced growth suppression, morphological changes, and loss of anchorage-independent growth of the tumor cells. As the wt-p53 gene apparently plays a negative role in growth regulation of cervical carcinoma cells, this gene may possibly be of some use for treating subjects with a cervical carcinoma.
Zheng
P S
PS
Department of Obstetrics and Gynecology, Saga Medical School, Japan.
Iwasaka
T
T
Ouchida
M
M
Fukuda
K
K
Yokoyama
M
M
Sugimori
H
H
D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology*; D000704:Analysis of Variance; D000818:Animals; D001483:Base Sequence; D015197:Carcinogenicity Tests; D002455:Cell Division / Q000235:genetics; D002999:Clone Cells; D018076:DNA, Complementary / Q000032:analysis; D004273:DNA, Neoplasm / Q000032:analysis; D005260:Female; D015870:Gene Expression; D016158:Genes, p53; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D008969:Molecular Sequence Data; D009368:Neoplasm Transplantation; D015345:Oligonucleotide Probes / Q000235:genetics; D010641:Phenotype; D014162:Transfection; D014407:Tumor Cells, Cultured / Q000473:pathology; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000473:pathology*
D018076:DNA, Complementary; D004273:DNA, Neoplasm; D015345:Oligonucleotide Probes