An improved technique for the cryopreservation of Gaigeria pachyscelis (Sandveld hookworm).
A technique for the cryopreservation of third-stage larvae of Gaigeria pachyscelis is described. It consists of incubating sheathed infective larvae at 37 degrees C in 40% (v/v) ethylene glycol for 7 min, followed by 2 min at 0 degree C in 82.8% (v/v) cryoprotectant mixture, prior to transferring the larvae to liquid nitrogen. The survival rate obtained with this technique is consistently high: 69.1% as assessed by motility.
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Helminthology Division, Onderstepoort Veterinary Institute, South Africa.
D000723:Ancylostomatoidea; D000818:Animals; D015925:Cryopreservation / Q000379:methods*; D007814:Larva; D013019:South Africa