Two transcripts with different sizes derived from a rice homeobox gene, OSH1.
A rice homeobox gene, OSH1, contains two functionally independent promoters which generate a larger transcript and a smaller transcript. In Arabidopsis, each promoter can drive the expression of a reporter gene in a different manner, indicating that the expression of different sized transcripts is independently regulated by each promoter. Over-expression of the larger transcript in transformed plants caused altered morphologies (Matsuoka et al., Plant Cell, 1993, 5, 1039-1048); in contrast, over-expression of the smaller transcript did not cause any morphological changes. The results suggest that the product of the smaller transcript fails to alter the expression of its target gene(s) in the transformants, while that of the larger transcript is capable of altering the expression of its target gene(s).
Tamaoki
M
M
BioScience Center, Nagoya University, Japan.
Ichikawa
H
H
Kayano
T
T
Kano-Murakami
Y
Y
Yamamoto
N
N
Matsuoka
M
M
D000595:Amino Acid Sequence; D001483:Base Sequence; D005091:Exons; D005801:Genes, Homeobox; D005966:Glucuronidase / Q000235:genetics; D018398:Homeodomain Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000235:genetics; D015534:Trans-Activators / Q000235:genetics*
D018398:Homeodomain Proteins; C099194:OSH1 protein, Oryza sativa; D010940:Plant Proteins; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D015534:Trans-Activators; D005966:Glucuronidase