Molecular cloning and nucleotide sequencing of the 3'-terminal region of a South African isolate of ryegrass mosaic virus RNA and in vitro expression of the coat protein gene.
The 2094 nucleotides at the 3'-terminus of a South African isolate of ryegrass mosaic virus (RGMV) was cloned and sequenced. Two putative polyprotein cleavage sites were found: Q/L and E/A, both of which are novel in the Potyviridae. The RGMV-SA cDNA was cloned into an expression vector, pUEX, and a fusion protein of 185 kDa was obtained which reacted strongly to anti-RGMV-SA antiserum. Alignment of the predicted amino acid sequence of RGMV-SA with those of other Potyviridae members showed limited identity, indicating that RGMV-SA is a definite and distinct virus.
Salm
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Range and Forage Institute, University of the Witwatersrand, Johannesburg, Pretoria, South Africa.
Rey
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French
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D000595:Amino Acid Sequence; D001483:Base Sequence; D002213:Capsid / Q000096:biosynthesis* / Q000235:genetics; D017124:Conserved Sequence; D017931:DNA Primers; D018076:DNA, Complementary; D015870:Gene Expression; D005814:Genes, Viral; D008129:Lolium / Q000821:virology*; D008969:Molecular Sequence Data; D009029:Mosaic Viruses / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D013019:South Africa
D017931:DNA Primers; D018076:DNA, Complementary; D012367:RNA, Viral