title: rK39: a cloned antigen of Leishmania chagasi that predicts active visceral leishmaniasis.
abstract: The diagnosis of visceral leishmaniasis (VL), a serious and often fatal parasitic disease caused by members of the Leishmania donovani complex, remains problematic. Current methods rely on clinical criteria, parasite identification in aspirate material, and serology. The latter methods use crude antigen preparations lacking in specificity. A previously described cloned antigen, rK39, of Leishmania specific for all members of the L. donovani complex (L. chagasi, L. donovani, L. infantum) was very useful in the serodiagnosis by ELISA of both human and canine VL. The present study demonstrated that rK39 seroreactivity correlated with active disease. The sera from early or self-healing infected subjects reacted with leishmanial lysate and were generally nonreactive with rK39. These data demonstrate the utility of rK39 in the serodiagnosis of VL and as an indicator of active disease.
authors.affiliation: Federal University of Bahia, Salvador, Brazil.
authors.forename: R
authors.initials: R
authors.lastname: Badaró
authors.forename: D
authors.initials: D
authors.lastname: Benson
authors.forename: M C
authors.initials: MC
authors.lastname: Eulálio
authors.forename: M
authors.initials: M
authors.lastname: Freire
authors.forename: S
authors.initials: S
authors.lastname: Cunha
authors.forename: E M
authors.initials: EM
authors.lastname: Netto
authors.forename: D
authors.initials: D
authors.lastname: Pedral-Sampaio
authors.forename: C
authors.initials: C
authors.lastname: Madureira
authors.forename: J M
authors.initials: JM
authors.lastname: Burns
authors.forename: R L
authors.initials: RL
authors.lastname: Houghton
authors.forename: J R
authors.initials: JR
authors.lastname: David
authors.forename: S G
authors.initials: SG
authors.lastname: Reed
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D000918:Antibody Specificity; D000953:Antigens, Protozoan / Q000235:genetics; D015415:Biomarkers; D001938:Brazil / Q000453:epidemiology; D002648:Child; D003001:Cloning, Molecular; D003429:Cross Reactions; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D018314:Leishmania infantum / Q000235:genetics / Q000276:immunology*; D007898:Leishmaniasis, Visceral / Q000175:diagnosis* / Q000453:epidemiology / Q000276:immunology*; D015800:Protozoan Proteins / Q000235:genetics / Q000276:immunology*; D012698:Serologic Tests
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D015415:Biomarkers; D015800:Protozoan Proteins; C079253:K39 antigen, Leishmania