title: Evidence for sltA1 as a salt-sensitive allele of the arginase gene (agaA) in the ascomycete Aspergillus nidulans.
abstract: Strains of Aspergillus nidulans carrying the sltA1 mutation, conferring sensitivity to KCl and NaCl, also showed an arginine-sensitive phenotype whereby concentrations of the L-amino acid at or above 10 mM were toxic to growth. Sexual progeny of a cross between a sltA1 mutant and a wild-type strain showed a co-segregation of salt and arginine sensitivity. Similarly, revertants to salt tolerance showed a loss of arginine sensitivity as did sltA1 strains that were transformed with a cosmid carrying the putative sltA1+ wild-type allele. In addition, arginine sensitivity could be relieved by L-ornithine. It is suggested that sltA1 is a salt-sensitive allele of the arginase gene (agaA).
authors.affiliation: School of Applied Sciences, University of Wolverhampton, Wulfruna Street, Wolverhampton, West Midlands, WV1 1LY, UK.
authors.forename: D J
authors.initials: DJ
authors.lastname: Clement
authors.forename: M S
authors.initials: MS
authors.lastname: Stanley
authors.forename: N A
authors.initials: NA
authors.lastname: Attwell
authors.forename: N J
authors.initials: NJ
authors.lastname: Clipson
authors.forename: D A
authors.initials: DA
authors.lastname: Fincham
authors.forename: P
authors.initials: P
authors.lastname: Hooley
mesh_terms: D000483:Alleles; D001119:Arginase / Q000235:genetics*; D001120:Arginine / Q000031:analogs & derivatives / Q000494:pharmacology; D001233:Aspergillus nidulans / Q000187:drug effects / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D002712:Chlorides / Q000494:pharmacology*; D003433:Crosses, Genetic; D004352:Drug Resistance, Microbial; D005800:Genes, Fungal; D008540:Meiosis; D016296:Mutagenesis; D010641:Phenotype; D011189:Potassium Chloride / Q000494:pharmacology; D011523:Protoplasts / Q000187:drug effects; D012965:Sodium Chloride / Q000494:pharmacology; D014170:Transformation, Genetic
chemical_list: D002712:Chlorides; D012965:Sodium Chloride; D011189:Potassium Chloride; D001120:Arginine; D001119:Arginase