title: Cloning and characterization of an Arabidopsis thaliana cDNA clone encoding an organellar isoform of serine acetyltransferase.
abstract: We have cloned an Arabidopsis thaliana cDNA encoding serine acetyltransferase (EC 2.3.1.30) by functional complementation of the Escherichia coli cysE mutant JM15. The cDNA clone Sat-1 conferred serine acetyltransferase activity (with apparent Km for the two substrates acetyl CoA and L-serine of 0.043 and 3.47 mmol/dm3 respectively) on the cysE mutant. The 1515 bp full-length cDNA encodes a deduced protein of 391 amino acids which includes a putative chloroplastic targeting presequence. Northern analysis revealed a single message of 1.5 kb, while Southern hybridisation suggests a small multigene family of related sequences.
authors.affiliation: Plant Sciences Laboratory, Research Division of Environmental and Evolutionary Biology, School of Biological and Medical Sciences, University of St. Andrews, Fife, UK.
authors.forename: M A
authors.initials: MA
authors.lastname: Roberts
authors.forename: J L
authors.initials: JL
authors.lastname: Wray
mesh_terms: D000123:Acetyltransferases / Q000235:genetics*; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D015388:Organelles / Q000201:enzymology*; D017386:Sequence Homology, Amino Acid; D051050:Serine O-Acetyltransferase
chemical_list: D018076:DNA, Complementary; D000123:Acetyltransferases; D051050:Serine O-Acetyltransferase