title: A Leishmania major protein with extensive homology to silent information regulator 2 of Saccharomyces cerevisiae.
abstract: We have isolated a cDNA from the protozoan parasite Leishmania major (Lm) that encodes a protein homologous to the Saccharomyces cerevisiae and Kluyveromyces marxianus silent information regulator 2 (SIR2) proteins. The deduced Lm SIR2-related protein (termed LmSIR2rp) consists of 381 amino acids that share 40.5% identity with yeast SIR2, increasing to 60% when substitutions are included. Moreover, the LmSIR2rp aa sequence contains a single potential zinc-binding domain with a CysXaa2CysXaa20CysXaa2Cys motif, and its C-terminal part is rich in Ser (16 Ser residues over 40 aa) which constitute potential sites for phosphorylation. The characterization of a novel Lm gene product which shows considerable similarity to a yeast mating-type regulatory protein provides a new tool to investigate the parasite differentiation control mechanisms and gene expression regulation.
authors.affiliation: Laboratoire de Recherche sur les Trypanosomatidae, INSERM U 415, Institut Pasteur, Lille, France.
authors.forename: B
authors.initials: B
authors.lastname: Yahiaoui
authors.forename: A
authors.initials: A
authors.lastname: Taibi
authors.forename: A
authors.initials: A
authors.lastname: Ouaissi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000276:immunology; D005656:Fungal Proteins; D017125:Genes, Protozoan; D006655:Histone Deacetylases; D018320:Leishmania major / Q000235:genetics*; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D038281:Silent Information Regulator Proteins, Saccharomyces cerevisiae; D056565:Sirtuin 2; D037761:Sirtuins; D015534:Trans-Activators / Q000235:genetics* / Q000276:immunology; D016335:Zinc Fingers
chemical_list: D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D010455:Peptides; D038281:Silent Information Regulator Proteins, Saccharomyces cerevisiae; D015534:Trans-Activators; C087778:SIR2 protein, S cerevisiae; C447944:Sir2 protein, Kluyveromyces lactis; D056565:Sirtuin 2; D037761:Sirtuins; D006655:Histone Deacetylases