The hydration of proteins in solutions by self-diffusion coefficients. NMR study.
The hydration of the globular (lysozyme, albumin) and fibrillar (fibrinogen) proteins in solution has been determined from the measurements of the self-diffusion coefficient by NMR pulsed gradient method. It has been concluded that the concentration dependencies of the self-diffusion coefficient of water molecules in protein solution are controlled by two additive factors: obstruction effect correlated with the protein dimension and direct hydration effect proportional to the number of the adsorption sites on the protein surface.
Baranowska
H M
HM
Department of Physics, Agricultural University of Poznań, Poland.
Olszewski
K J
KJ
D000818:Animals; D001665:Binding Sites; D002417:Cattle; D002645:Chickens; D004058:Diffusion; D005340:Fibrinogen / Q000737:chemistry; D006801:Humans; D066298:In Vitro Techniques; D009682:Magnetic Resonance Spectroscopy; D008956:Models, Chemical; D009113:Muramidase / Q000737:chemistry; D011506:Proteins / Q000737:chemistry*; D012709:Serum Albumin / Q000737:chemistry; D012996:Solutions; D014867:Water / Q000737:chemistry
D011506:Proteins; D012709:Serum Albumin; D012996:Solutions; D014867:Water; D005340:Fibrinogen; D009113:Muramidase