Specification of pituitary cell lineages by the LIM homeobox gene Lhx3.
During pituitary organogenesis, the progressive differentiation of distinct pituitary-specific cell lineages from a common primordium involves a series of developmental decisions and inductive interactions. Targeted gene disruption in mice showed that Lhx3, a LIM homeobox gene expressed in the pituitary throughout development, is essential for differentiation and proliferation of pituitary cell lineages. In mice homozygous for the Lhx3 mutation, Rathke's pouch formed but failed to grow and differentiate; such mice lacked both the anterior and intermediate lobes of the pituitary. The determination of all pituitary cell lineages, except the corticotrophs, was affected, suggesting that a distinct, Lhx3-independent ontogenetic pathway exists for the initial specification of this lineage.
Sheng
H Z
HZ
Laboratory of Mammalian Genes and Development, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA.
Zhadanov
A B
AB
Mosinger
B
B
Fujii
T
T
Bertuzzi
S
S
Grinberg
A
A
Lee
E J
EJ
Huang
S P
SP
Mahon
K A
KA
Westphal
H
H
D000818:Animals; D002352:Carrier Proteins / Q000235:genetics; D002454:Cell Differentiation; D019070:Cell Lineage; D005314:Embryonic and Fetal Development; D018507:Gene Expression Regulation, Developmental; D018390:Gene Targeting; D005801:Genes, Homeobox; D015292:Glycoprotein Hormones, alpha Subunit / Q000096:biosynthesis / Q000235:genetics; D018398:Homeodomain Proteins / Q000235:genetics*; D060850:LIM-Homeodomain Proteins; D008565:Membrane Proteins; D051379:Mice; D009154:Mutation; D049294:Phospholipid Transfer Proteins; D010902:Pituitary Gland / Q000002:abnormalities / Q000166:cytology* / Q000196:embryology; D010903:Pituitary Gland, Anterior / Q000002:abnormalities / Q000166:cytology* / Q000196:embryology; D011333:Pro-Opiomelanocortin / Q000096:biosynthesis / Q000235:genetics; D014157:Transcription Factors
D002352:Carrier Proteins; D015292:Glycoprotein Hormones, alpha Subunit; D018398:Homeodomain Proteins; D060850:LIM-Homeodomain Proteins; C094898:Lhx3 protein; D008565:Membrane Proteins; D049294:Phospholipid Transfer Proteins; D014157:Transcription Factors; D011333:Pro-Opiomelanocortin