Binding and functional effects of transcriptional factor Sp1 on the murine interleukin-6 promotor.
The NF-kappaB and NF-IL6 elements have previously been shown to play an important role in regulation of both the mouse and human interleukin-6 gene. Between these two elements lies a G/C-rich sequence, which contains three repeats of the element CCACC, protein binding to which has not been previously characterized. In this study we demonstrate that the transcription factor Sp1 binds to these repeats and plays an important role in basal and in inducible expression of the murine interleukin-6 gene.
Kang
S H
SH
Lynx Therapeutics, Hayward, California 94545, USA.
Brown
D A
DA
Kitajima
I
I
Xu
X
X
Heidenreich
O
O
Gryaznov
S
S
Nerenberg
M
M
D016475:3T3 Cells; D000818:Animals; D001402:B-Lymphocytes; D001483:Base Sequence; D001665:Binding Sites; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D018983:DNA Footprinting; D017931:DNA Primers; D005347:Fibroblasts; D015870:Gene Expression; D016355:Genes, pX; D015850:Interleukin-6 / Q000096:biosynthesis* / Q000235:genetics*; D008156:Luciferases / Q000096:biosynthesis; D051379:Mice; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000096:biosynthesis; D012091:Repetitive Sequences, Nucleic Acid; D016329:Sp1 Transcription Factor / Q000378:metabolism*; D014162:Transfection
D017931:DNA Primers; D015850:Interleukin-6; D011994:Recombinant Proteins; D016329:Sp1 Transcription Factor; D008156:Luciferases