title: A feedback-controlled pump produces stable hypotension in anaesthetised rabbits.
abstract: A method is described for the reliable production of controlled hypotension in experimental animals. Reduction in arterial blood pressure was obtained in rabbits by withdrawing arterial blood using a computer-driven pump operating within a feedback control system. Arterial blood pressure, blood flow velocity in the basilar artery (measured using transcranial Doppler), and anterior cortical microcirculation (measured using laser Doppler) were monitored. The aim of the experiments was to compare stability of hypotension produced using arterial blood pressure or basilar flow velocity as feedback control variables. Basilar artery flow velocity provided the most stable profound hypotension and during reinfusion when animals were not autoregulating. However, arterial blood pressure provided the most accurate stepwise control in autoregulating animals.
authors.affiliation: MRC Cambridge Centre for Brain Repair, Addenbrooke's Hospital, University of Cambridge, England.
authors.forename: P
authors.initials: P
authors.lastname: Smielewski
authors.forename: P J
authors.initials: PJ
authors.lastname: Kirkpatrick
authors.forename: H
authors.initials: H
authors.lastname: Richards
authors.forename: J D
authors.initials: JD
authors.lastname: Pickard
authors.forename: M
authors.initials: M
authors.lastname: Czosnyka
mesh_terms: D000758:Anesthesia; D000818:Animals; D001488:Basilar Artery / Q000503:physiopathology; D001783:Blood Flow Velocity; D002540:Cerebral Cortex / Q000098:blood supply; D002560:Cerebrovascular Circulation; D004195:Disease Models, Animal; D005246:Feedback; D005260:Female; D007022:Hypotension / Q000209:etiology* / Q000503:physiopathology*; D007260:Infusion Pumps; D017078:Laser-Doppler Flowmetry; D008297:Male; D008833:Microcirculation; D011817:Rabbits; D015203:Reproducibility of Results