Identification of a HeLa mRNA fraction which corrects the mitomycin C sensitivity of irs1 cells.
The hamster cell mutant irs1 is defective in its response to DNA lesions caused by a variety of mutagens, particularly cross-linking agents. These cells have been assigned to complementation group 2 of X-ray-sensitive mutants and the mutated gene is called XRCC2(X-ray repair cross complementing). We have identified, by microinjection, a human mRNA fraction which can transiently correct the sensitivity of these cells to cross-linking agents. This fraction contains mRNAs of 3.5 kb (+/- 0.25) including, therefore, the transcript of the XRCC2 gene.
Bender
O
O
Institut für Humangenetik, Virchow Klinikum, Humboldt Universität zu Berlin, Germany.
Jones
N J
NJ
Sperling
K
K
Digweed
M
M
D000818:Animals; D002470:Cell Survival; D006224:Cricetinae; D004260:DNA Repair / Q000235:genetics*; D004305:Dose-Response Relationship, Drug; D005816:Genetic Complementation Test; D006367:HeLa Cells; D006801:Humans; D008845:Microinjections; D016685:Mitomycin / Q000494:pharmacology*; D009153:Mutagens / Q000494:pharmacology; D012333:RNA, Messenger / Q000302:isolation & purification*; D011836:Radiation Tolerance / Q000235:genetics
D009153:Mutagens; D012333:RNA, Messenger; D016685:Mitomycin