title: Epidermal urocanic acid and suppression of contact hypersensitivity by ultraviolet radiation in Monodelphis domestica.
abstract: A single specific epidermal photoreceptor for the immunosuppressive action of UV radiation has not been defined, although separate evidence is accruing in favour of each of two candidates, trans-urocanic acid and DNA. In Monodelphis domestica, specific photoreactivation repair of UV radiation-induced pyrimidine dimers has been shown to abrogate the suppression of contact hypersensitivity (CHS), thus suggesting that DNA is the target for this impairment. However, the both haired and hairless mice, immunosuppressive effects of UV radiation have been reproduced by the exogenous administration of the UV photoproduct of urocanic acid, cis-urocanic acid. We show here that the epidermis of M. domestica contains urocanic acid, that UV irradiation of the shaved dorsal skin has resulted in an increase in epidermal cis-urocanic acid and that the topical application of a cis-urocanic acid-containing lotion significantly depressed the capacity of Monodelphis to respond to contact sensitisers, in a manner analogous to these responses in the hairless mouse. Therefore in Monodelphis, suppression of CHS by UV irradiation appears to involve both urocanic acid photo-isomerisation and epidermal DNA damage.
authors.affiliation: Department of Veterinary Pathology, University of Sydney, Australia.
authors.forename: V E
authors.initials: VE
authors.lastname: Reeve
authors.forename: R D
authors.initials: RD
authors.lastname: Ley
authors.forename: W G
authors.initials: WG
authors.lastname: Reilly
authors.forename: M
authors.initials: M
authors.lastname: Bosnic
mesh_terms: D000287:Administration, Topical; D000818:Animals; D003877:Dermatitis, Contact / Q000209:etiology / Q000276:immunology*; D004817:Epidermis / Q000737:chemistry* / Q000276:immunology; D005260:Female; D007166:Immunosuppressive Agents / Q000008:administration & dosage / Q000494:pharmacology / Q000528:radiation effects; D008297:Male; D051379:Mice; D008819:Mice, Nude; D009893:Opossums; D013237:Stereoisomerism; D014466:Ultraviolet Rays; D014560:Urocanic Acid / Q000008:administration & dosage / Q000494:pharmacology / Q000528:radiation effects*
chemical_list: D007166:Immunosuppressive Agents; D014560:Urocanic Acid