A Ca2+/CAM protein kinase associated with Ca2+ transport in sarco(endo)plasmic vesicles from tracheal smooth muscle.
In this work, we show evidence to support the existence of a Ca2+ calmodulin (CAM) dependent protein kinase and a substrate, a 17 kilodaltons (KDA) polypeptide being both associated to sarco(endo)plasmic reticulum vesicles from tracheal smooth muscle. Anti-CAM drugs such as compound 48/80 inhibited this protein kinase activity and this inhibition was reversed in the presence of Ca2+CAM. Moreover, as a result of this phosphorylation, there is a significant increase in the ATP dependent Ca2+ transport in these sarco(endo)plasmic vesicles.
de Alfonzo
R G
RG
Sección de Biomembranas, Universidad Central de Venezuela, Sabana Grande, Caracas, Venezuela.
de Becemberg
I L
IL
Alfonzo
M J
MJ
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000378:metabolism*; D002417:Cattle; D004721:Endoplasmic Reticulum / Q000201:enzymology / Q000378:metabolism*; D017136:Ion Transport; D009130:Muscle, Smooth / Q000201:enzymology / Q000378:metabolism*; D010766:Phosphorylation; D012519:Sarcoplasmic Reticulum / Q000201:enzymology / Q000378:metabolism*; D013347:Subcellular Fractions / Q000201:enzymology; D013379:Substrate Specificity
D000255:Adenosine Triphosphate; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D002118:Calcium