title: Blockage by adenovirus E4orf6 of transcriptional activation by the p53 tumor suppressor.
abstract: The adenovirus E4orf6 protein is shown here to interact with the cellular tumor suppressor protein p53 and to block p53-mediated transcriptional activation. The adenovirus protein inhibited the ability of p53 to bind to human TAFII31, a component of transcription factor IID (TFIID). Earlier work demonstrated that the interaction of p53 with TAFII31 involves a sequence near the NH2-terminus of p53, whereas the E4orf6-p53 interaction occurs within amino acids 318 to 360 of p53. Thus, the E4orf6 protein interacts at a site on p53 distinct from the domain that binds to TAFII31 but nevertheless inhibits the p53-TAFII31 interaction.
authors.affiliation: Institut für Medizinische Mikrobiologie und Hygiene, Universität Regensburg, Germany.
authors.forename: T
authors.initials: T
authors.lastname: Dobner
authors.forename: N
authors.initials: N
authors.lastname: Horikoshi
authors.forename: S
authors.initials: S
authors.lastname: Rubenwolf
authors.forename: T
authors.initials: T
authors.lastname: Shenk
mesh_terms: D000256:Adenoviridae / Q000502:physiology; D017504:Adenovirus E4 Proteins / Q000276:immunology / Q000378:metabolism*; D002460:Cell Line; D004247:DNA / Q000378:metabolism; D016158:Genes, p53; D006367:HeLa Cells; D006801:Humans; D015151:Immunoblotting; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D035381:TATA-Binding Protein Associated Factors; D015534:Trans-Activators / Q000378:metabolism*; D035362:Transcription Factor TFIID; D015533:Transcriptional Activation; D014162:Transfection; D016159:Tumor Suppressor Protein p53 / Q000737:chemistry / Q000378:metabolism*
chemical_list: D017504:Adenovirus E4 Proteins; D011993:Recombinant Fusion Proteins; C106849:TAF9 protein, human; D035381:TATA-Binding Protein Associated Factors; D015534:Trans-Activators; D035362:Transcription Factor TFIID; D016159:Tumor Suppressor Protein p53; D004247:DNA