title: Cooperative oxygen binding to scapharca inaequivalvis hemoglobin in the crystal.
abstract: Oxygen binding to homodimeric Scapharca inaequivalvis hemoglobin (HbI) crystals has been investigated by single-crystal polarized absorption microspectrophotometry. The saturation curve, characterized by a Hill coefficient nH = 1.45 and an oxygen pressure at half saturation p50 = 4.8 torr, at 15 degrees C, shows that HbI in the crystalline state retains positive cooperativity in ligand binding. This finding will permit the correlation of the oxygen-linked conformational changes in the crystal with the expression of cooperativity. Polarized absorption spectra of deoxy-HbI, oxy-HbI, and oxidized HbI crystals indicate that oxygenation does not induce heme reorientation, whereas oxidation does. Lattice interactions prevent the dissociation of oxidized dimers that occurs in solution and stabilize an equilibrium distribution of pentacoordinate and hexacoordinate high spin species.
authors.affiliation: Istituto di Scienze Biochimiche, Università di Parma, 43100 Parma, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Mozzarelli
authors.forename: S
authors.initials: S
authors.lastname: Bettati
authors.forename: C
authors.initials: C
authors.lastname: Rivetti
authors.forename: G L
authors.initials: GL
authors.lastname: Rossi
authors.forename: G
authors.initials: G
authors.lastname: Colotti
authors.forename: E
authors.initials: E
authors.lastname: Chiancone
mesh_terms: D000818:Animals; D049872:Bivalvia; D003460:Crystallization; D006454:Hemoglobins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D046911:Macromolecular Substances; D009994:Osmolar Concentration; D010084:Oxidation-Reduction; D010100:Oxygen; D010108:Oxyhemoglobins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D012996:Solutions; D013053:Spectrophotometry
chemical_list: D006454:Hemoglobins; D046911:Macromolecular Substances; D010108:Oxyhemoglobins; D012996:Solutions; C083082:dimeric hemoglobin, Scapharca inaequivalvis; D010100:Oxygen