title: Genetic variants of human T-lymphotrophic virus type II in American Indian groups.
abstract: The human T-lymphotropic virus type II (HTLV-II) is found in many New World Indian groups in North and South America and may have entered the New World from Asia with the earliest migration of ancestral Amerindians over 15,000 years ago. To characterize the phylogenetic relationships of HTLV-II strains infecting geographically diverse Indian populations, we used polymerase chain reaction to amplify HTLV-II sequences from lymphocytes of seropositive Amerindians from Brazil (Kraho, Kayapo, and Kaxuyana), Panama (Guaymi), and the United States (the Navajo and Pueblo tribes of the southwestern states and the Seminoles of Florida). Sequence analysis of a 780-base pair fragment (located between the env gene and the second exons of tax/rex) revealed that Amerindian viruses clustered in the same two genetic subtypes (IIa and IIb) previously identified for viruses from intravenous drug users. Most infected North and Central American Indians had subtype IIb, while HTLV-II infected members of three remote Amazonian tribes clustered as a distinct group within subtype IIa. These findings suggest that the ancestral Amerindians migrating to the New World brought at least two genetic subtypes, IIa and IIb. Because HTLV-II strains from Amazonian Indians form a distinct group within subtype HTLV-IIa, these Brazilian tribes are unlikely to be the source of IIa viruses in North American drug users. Finally, the near identity of viral sequences from geographically diverse populations indicate that HTLV-II is a very ancient virus of man.
authors.affiliation: Viral Epidemiology Branch, National Cancer Institute, Bethesda, Maryland, USA.
authors.forename: R J
authors.initials: RJ
authors.lastname: Biggar
authors.forename: M E
authors.initials: ME
authors.lastname: Taylor
authors.forename: J V
authors.initials: JV
authors.lastname: Neel
authors.forename: B
authors.initials: B
authors.lastname: Hjelle
authors.forename: P H
authors.initials: PH
authors.lastname: Levine
authors.forename: F L
authors.initials: FL
authors.lastname: Black
authors.forename: G M
authors.initials: GM
authors.lastname: Shaw
authors.forename: P M
authors.initials: PM
authors.lastname: Sharp
authors.forename: B H
authors.initials: BH
authors.lastname: Hahn
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D004279:DNA, Viral; D014644:Genetic Variation; D015367:Human T-lymphotropic virus 2 / Q000145:classification / Q000235:genetics*; D006801:Humans; D007198:Indians, North American; D008969:Molecular Sequence Data; D010802:Phylogeny; D012689:Sequence Homology, Nucleic Acid
chemical_list: D004279:DNA, Viral