title: Relationship between nuclease-hypersensitive sites and meiotic recombination hot spot activity at the HIS4 locus of Saccharomyces cerevisiae.
abstract: Meiotic double-strand DNA breaks (DSBs), the lesions that initiate meiotic recombination at the HIS4 recombination hot spot, occur in a region upstream of the coding sequence associated with multiple DNase I-hypersensitive sites. Mutations in transcription factors that lead to loss of the DSBs result in the loss of some but not all DNase I-hypersensitive sites in the upstream region. A meiosis-specific change in chromatin structure is detected in strains with the wild-type hot spot but not in strains with alterations that elevate or reduce hot spot activity. The position and intensity of micrococcal nuclease-hypersensitive sites correlate poorly with the sites of DSB formation.
authors.affiliation: Department of Biology, University of North Carolina, Chapel Hill, 27599-3280, USA.
authors.forename: Q Q
authors.initials: QQ
authors.lastname: Fan
authors.forename: T D
authors.initials: TD
authors.lastname: Petes
mesh_terms: D000429:Alcohol Oxidoreductases; D000619:Aminohydrolases; D002843:Chromatin / Q000502:physiology; D004271:DNA, Fungal / Q000378:metabolism*; D003850:Deoxyribonuclease I; D005656:Fungal Proteins / Q000235:genetics*; D005838:Genotype; D008540:Meiosis; D008938:Mitosis; D009097:Multienzyme Complexes / Q000235:genetics; D011401:Promoter Regions, Genetic; D011755:Pyrophosphatases; D011995:Recombination, Genetic; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
chemical_list: D002843:Chromatin; D004271:DNA, Fungal; D005656:Fungal Proteins; D009097:Multienzyme Complexes; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D000429:Alcohol Oxidoreductases; C092116:HIS4 protein, S cerevisiae; D003850:Deoxyribonuclease I; D000619:Aminohydrolases; D011755:Pyrophosphatases