title: Efficacy of hypochlorous acid scavengers in the prevention of protein carbonyl formation.
abstract: We observed that protein (bovine serum albumin) carbonyl content increases upon hypochlorite oxidation, and this increase is inhibited in a concentration-dependent manner in the presence of hypochlorite scavengers. Based on this observation, we tested whether some known hypochlorite scavengers (lipoic acid, cysteine, and glutathione) and some other antioxidants (uric acid, ascorbic acid, alpha-tocopherol, and probucol) could prevent protein carbonyl formation. N-acetylcysteine, dihydrolipoic acid, cysteine, and glutathione (reduced form, GSH), which otherwise could not be tested in a previously reported 5-thio-2-nitrobenzoic acid test system, were successfully evaluated in our assay. The hypochlorite scavenging capacity of different compounds, compared by determining the IC50 (concentration which produces 50% inhibition), showed that the compounds tested have the following potency: dihydrolipoic acid > GSH, N-acetylcysteine > cysteine > S-methyl glutathione > lipoic acid, ascorbic acid > cystine, GSSG, and uric acid. No scavenging ability was observed for either alpha-tocopherol or probucol.
authors.affiliation: Department of Molecular and Cell Biology, University of California, Berkeley, 94720-3200, USA.
authors.forename: L J
authors.initials: LJ
authors.lastname: Yan
authors.forename: M G
authors.initials: MG
authors.lastname: Traber
authors.forename: H
authors.initials: H
authors.lastname: Kobuchi
authors.forename: S
authors.initials: S
authors.lastname: Matsugo
authors.forename: H J
authors.initials: HJ
authors.lastname: Tritschler
authors.forename: L
authors.initials: L
authors.lastname: Packer
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000494:pharmacology; D002417:Cattle; D003545:Cysteine / Q000494:pharmacology; D016166:Free Radical Scavengers / Q000494:pharmacology*; D005978:Glutathione / Q000494:pharmacology; D006997:Hypochlorous Acid / Q000494:pharmacology*; D007700:Kinetics; D010084:Oxidation-Reduction; D011341:Probucol / Q000494:pharmacology; D012710:Serum Albumin, Bovine / Q000737:chemistry* / Q000187:drug effects; D008063:Thioctic Acid / Q000494:pharmacology; D014527:Uric Acid / Q000494:pharmacology; D014810:Vitamin E / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D016166:Free Radical Scavengers; D014810:Vitamin E; D014527:Uric Acid; D012710:Serum Albumin, Bovine; D006997:Hypochlorous Acid; D008063:Thioctic Acid; D005978:Glutathione; D003545:Cysteine; D011341:Probucol; D001205:Ascorbic Acid