Human trabecular meshwork cell survival is dependent on perfusion rate.
OBJECTIVE
To determine whether suppression of flow may be detrimental to trabecular cell survival and to the morphologic characteristics of the trabecular meshwork.


METHODS
The anterior segments of normal human eye bank eyes were placed in perfusion organ culture. The effect of various perfusion rates of culture medium, and of the constant flow and constant pressure methods of delivery of culture medium, were studied. Trabecular cell survival was determined by quantitation of cell nuclei in histologic sections and by morphologic observation.


RESULTS
Trabecular meshworks with perfusion rates of 1 microliter/minute and higher had significantly more trabecular cells than meshworks with lower perfusion rates. A significant loss of trabecular cells was found in meshworks cultured with the constant pressure technique when compared with fellow eyes cultured with the constant flow of medium. Those constant pressure cultured meshworks with surviving cells had higher flow rates than those with necrotic cells.


CONCLUSIONS
A minimum perfusion rate of 1 microliter/minute is required for trabecular cell survival in perfusion organ culture. Constant pressure perfusion of medium is unsuccessful in maintaining trabecular cells in long-term culture if low perfusion rates occur. Constant flow appears to mimic the in vivo situation more closely.
Johnson
D H
DH
Department of Ophthalmology, Mayo Clinic 55905, USA.
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000867:Anterior Chamber / Q000502:physiology; D001082:Aqueous Humor / Q000502:physiology; D002452:Cell Count; D002470:Cell Survival / Q000502:physiology*; D003470:Culture Media; D004247:DNA / Q000096:biosynthesis; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009924:Organ Culture Techniques; D010477:Perfusion; D014129:Trabecular Meshwork / Q000166:cytology / Q000502:physiology*
D003470:Culture Media; D004247:DNA