Study of genotoxic effects of antidiarrheal medicinal herbs on human cells in vitro.
The use of medicinal herbs has been a common practice in Asia but their genotoxic properties are little known. In the present study, genotoxic effects of three antidiarrheal herbs, guava leaf, mangosteen peel and pomegranate peel, were examined using established human cell lines, Raji and P3HR-1. Cells were treated with boiled-water extract of the herbs at various concentrations for 24 and 48 hours in vitro. Cell growth and viability were dose dependently reduced. No apparent chromosomal aberrations were induced by the treatment. Administration of pomegranate extract induced apoptotic DNA fragmentation. This genotoxicity test system is simple and convenient for the primary screening.
Settheetham
W
W
Department of Physiology, Faculty of Medicine, Srinakharinwirot University, Bangkok, Thailand.
Ishida
T
T
D000930:Antidiarrheals / Q000633:toxicity*; D002051:Burkitt Lymphoma; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002869:Chromosome Aberrations; D004273:DNA, Neoplasm / Q000187:drug effects; D005638:Fruit; D006801:Humans; D019684:Magnoliopsida / Q000633:toxicity*; D008516:Medicine, Chinese Traditional; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity*; D008517:Phytotherapy; D010936:Plant Extracts / Q000633:toxicity; D010946:Plants, Medicinal; D014407:Tumor Cells, Cultured
D000930:Antidiarrheals; D004273:DNA, Neoplasm; D009153:Mutagens; D010936:Plant Extracts