title: Inversely correlated expression of p16 and Rb protein in non-small cell lung cancers: an immunohistochemical study.
abstract: Cdk4-mediated phosphorylation of Rb protein is inhibited by p16, a product of a possible tumor suppressor gene. We examined the expression of p16 and Rb protein by means of immunohistochemistry in 61 non-small cell lung cancers and have demonstrated an inverse relationship between the expression of p16 and Rb protein: 28/30 specimens that did not stain for p16 stained for Rb and 21/31 p16-positive specimens did not stain for Rb. Only 1 of the p16-negative specimens had a mutation of exon 2 of the CDKN2 gene. Our results indirectly support the theory that p16 expression is negatively regulated by the functional Rb protein.
authors.affiliation: First Department of Surgery, Osaka University Medical School, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Sakaguchi
authors.forename: Y
authors.initials: Y
authors.lastname: Fujii
authors.forename: H
authors.initials: H
authors.lastname: Hirabayashi
authors.forename: H E
authors.initials: HE
authors.lastname: Yoon
authors.forename: Y
authors.initials: Y
authors.lastname: Komoto
authors.forename: T
authors.initials: T
authors.lastname: Oue
authors.forename: T
authors.initials: T
authors.lastname: Kusafuka
authors.forename: A
authors.initials: A
authors.lastname: Okada
authors.forename: H
authors.initials: H
authors.lastname: Matsuda
mesh_terms: D001483:Base Sequence; D002289:Carcinoma, Non-Small-Cell Lung / Q000737:chemistry* / Q000235:genetics; D002352:Carrier Proteins / Q000032:analysis* / Q000235:genetics; D019941:Cyclin-Dependent Kinase Inhibitor p16; D005260:Female; D016147:Genes, Tumor Suppressor; D006801:Humans; D007150:Immunohistochemistry; D008175:Lung Neoplasms / Q000737:chemistry* / Q000235:genetics; D008297:Male; D008969:Molecular Sequence Data; D009154:Mutation; D016160:Retinoblastoma Protein / Q000032:analysis*
chemical_list: D002352:Carrier Proteins; D019941:Cyclin-Dependent Kinase Inhibitor p16; D016160:Retinoblastoma Protein