Characterization of an N6-oxopropenyl-2'-deoxyadenosine adduct in malondialdehyde-modified DNA using liquid chromatography/electrospray ionization tandem mass spectrometry.
Malondialdehyde (MDA), a product of lipid peroxidation, causes mutations in bacterial and mammalian cells and cancer in rats. MDA reacts with deoxynucleosides in vitro and the monomeric adduct of MDA with deoxyguanosine (M1G-dR) is the major adduct. M1G-dR has been detected in rat and human liver. Random mutagenesis studies with MDA-modified DNA and recent 32P-postlabeling studies indicate that in addition to M1G-dR, adducts to deoxyadenosine may also be formed. We have utilized liquid chromatography coupled with electrospray ionization tandem mass spectrometry to characterize an N6-oxopropenyl-2'-deoxyadenosine adduct (M1A-dR) in calf DNA modified with MDA.
Chaudhary
A K
AK
A.B. Hancock Jr Memorial Laboratory for Cancer Research, Vanderbilt University School of Medicine, Nashville, TN 37232, USA.
Reddy
G R
GR
Blair
I A
IA
Marnett
L J
LJ
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D018736:DNA Adducts / Q000737:chemistry*; D003839:Deoxyadenosines / Q000378:metabolism*; D006801:Humans; D008315:Malondialdehyde / Q000378:metabolism*; D013058:Mass Spectrometry; D051381:Rats
D018736:DNA Adducts; D003839:Deoxyadenosines; C586683:N6-oxopropenyl-2'-deoxyadenosine; D008315:Malondialdehyde; C058118:2'-deoxyadenosine