Absence of siderophore-like activity in Legionella pneumophila supernatants.
Conflicting reports have been given as to the existence of a Legionella pneumophila siderophore. Hence, we rigorously examined the reported siderophore-like activity using the chrome azurol S indicator. Although chrome azurol S reactivity was detected in supernatants, control experiments indicate that it was due to cysteine in the media. When bacteria were cultured in media without cysteine, no siderophores were detected.
Liles
M R
MR
Department of Microbiology-Immunology, Northwestern University, Chicago, Illinois 60611, USA.
Cianciotto
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NP
D000818:Animals; D003470:Culture Media; D003545:Cysteine / Q000378:metabolism; D006168:Guinea Pigs; D062385:Hydroxybenzoates; D007202:Indicators and Reagents; D016952:Legionella pneumophila / Q000254:growth & development / Q000378:metabolism*; D017262:Siderophores / Q000378:metabolism*
D003470:Culture Media; D062385:Hydroxybenzoates; D007202:Indicators and Reagents; D017262:Siderophores; C015076:chrome azurol S; D003545:Cysteine