title: Genomic rearrangement in genome segment 12 of rice dwarf phytoreovirus.
abstract: The genome segment 12 (S12) of rice dwarf phytoreovirus (RDV) isolated from the Philippines (RDV-P) and of a variant (RDV-S-6) of RDV severe strain (RDV-S) migrated abnormally slower during polyacrylamide gel electrophoresis than that of the isolate maintained at Hokkaido University (RDV-H). Nucleotide sequence analysis revealed that rearrangement had occurred in these segments, affecting the open reading frame. A polypeptide encoded by S12 (Pns12) of RDV-P had a duplication of 28 amino acids while 1/3 of the carboxyl terminus of Pns12 was deleted in RDV-S-6 by premature termination due to a frameshift. RDV-S is always present in plants infected with the RDV-S-6 variant, suggesting that Pns12 of RDV-S-6 is defective. On the other hand, Pns12 of RDV-P was expressed and appeared to be functional in infected cells in spite of the duplication, as demonstrated by immunoblot analyses using antibody raised against Pns12 expressed in Escherichia coli.
authors.affiliation: Department of Agrobiology and Bioresources, Faculty of Agriculture, Hokkaido University, Sapporo, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Murao
authors.forename: I
authors.initials: I
authors.lastname: Uyeda
authors.forename: Y
authors.initials: Y
authors.lastname: Ando
authors.forename: I
authors.initials: I
authors.lastname: Kimura
authors.forename: P Q
authors.initials: PQ
authors.lastname: Cabauatan
authors.forename: H
authors.initials: H
authors.lastname: Koganezawa
mesh_terms: D001483:Base Sequence; D017931:DNA Primers; D015321:Gene Rearrangement; D016679:Genome, Viral; D008969:Molecular Sequence Data; D012275:Oryza / Q000821:virology; D012367:RNA, Viral / Q000235:genetics; D012087:Reoviridae / Q000235:genetics*
chemical_list: D017931:DNA Primers; D012367:RNA, Viral