Inversely correlated expression of p16 and Rb protein in non-small cell lung cancers: an immunohistochemical study.
Cdk4-mediated phosphorylation of Rb protein is inhibited by p16, a product of a possible tumor suppressor gene. We examined the expression of p16 and Rb protein by means of immunohistochemistry in 61 non-small cell lung cancers and have demonstrated an inverse relationship between the expression of p16 and Rb protein: 28/30 specimens that did not stain for p16 stained for Rb and 21/31 p16-positive specimens did not stain for Rb. Only 1 of the p16-negative specimens had a mutation of exon 2 of the CDKN2 gene. Our results indirectly support the theory that p16 expression is negatively regulated by the functional Rb protein.
Sakaguchi
M
M
First Department of Surgery, Osaka University Medical School, Japan.
Fujii
Y
Y
Hirabayashi
H
H
Yoon
H E
HE
Komoto
Y
Y
Oue
T
T
Kusafuka
T
T
Okada
A
A
Matsuda
H
H
D001483:Base Sequence; D002289:Carcinoma, Non-Small-Cell Lung / Q000737:chemistry* / Q000235:genetics; D002352:Carrier Proteins / Q000032:analysis* / Q000235:genetics; D019941:Cyclin-Dependent Kinase Inhibitor p16; D005260:Female; D016147:Genes, Tumor Suppressor; D006801:Humans; D007150:Immunohistochemistry; D008175:Lung Neoplasms / Q000737:chemistry* / Q000235:genetics; D008297:Male; D008969:Molecular Sequence Data; D009154:Mutation; D016160:Retinoblastoma Protein / Q000032:analysis*
D002352:Carrier Proteins; D019941:Cyclin-Dependent Kinase Inhibitor p16; D016160:Retinoblastoma Protein