H2O2 renders cells accessible to lysis by exogenous phospholipase A2: a novel mechanism for cell damage in inflammatory processes.
Phospholipase A2 (PLA2) and H2O2, secreted from activated inflammatory cells, play a central role in the tissue damage occurring in inflammatory processes. However, while exogenous PLA2 alone does not cause cell lysis, it readily does so when acting with H2O2. We have found that H2O2 degrades cell surface proteoglycans, thus rendering the membrane PL accessible to hydrolysis by exogenous PLA2. This novel mechanism introduces a role for cell surface proteoglycans in protection of cells from damage by pro-inflammatory agents, and may assign a central role for the combined action of H2O2 and PLA2 in inflammatory and bacteriocidal processes.
Dan
P
P
Department of Biochemistry, Hebrew-University-Hadassah Medical School, Jerusalem, Israel.
Nitzan
D W
DW
Dagan
A
A
Ginsburg
I
I
Yedgar
S
S
D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D002470:Cell Survival; D002522:Chlorocebus aethiops; D002847:Chromatography, Agarose; D002857:Chromium / Q000378:metabolism; D005949:Glucose Oxidase / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism / Q000494:pharmacology*; D007668:Kidney; D008081:Liposomes / Q000378:metabolism; D008563:Membrane Lipids / Q000378:metabolism*; D010741:Phospholipases A / Q000378:metabolism*; D054467:Phospholipases A2; D010743:Phospholipids / Q000378:metabolism*; D011509:Proteoglycans / Q000378:metabolism*; D013849:Thimerosal / Q000494:pharmacology
D008081:Liposomes; D008563:Membrane Lipids; D010743:Phospholipids; D011509:Proteoglycans; D002857:Chromium; D013849:Thimerosal; D016718:Arachidonic Acid; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase; D010741:Phospholipases A; D054467:Phospholipases A2