title: The putative acetyl-CoA synthetase gene of Cryptosporidium parvum and a new conserved protein motif in acetyl-CoA synthetases.
abstract: We determined the nucleotide (nt) sequence of the putative gene encoding acetyl-coenzyme A synthetase (ACS) from the parasitic protozoan Cryptosporidium parvum. The gene is single copy, located on a chromosome of approximately 1.08 mb, and has no introns. The gene is characterized by low codon usage bias and encodes a 694-amino acid (aa) protein with a predicted molecular size of 78 kDa, similar to other ACSs from different prokaryotic and eukaryotic species. Comparison of multiple protein alignments of ACSs revealed a new conserved sequence motif PKT(R/V/L)SGK(I/V/T)(T/M/V/K)R(R/N) near the C-terminus, which may be a signature for ACSs. This motif shares significant homology with sequences from other members of the AMP-binding family, has secondary structure similar to the purine-binding motif of ATP- and GTP-ases, and may play a role in the enzymatic activity of proteins from the AMP-binding family.
authors.affiliation: Division of Biology, Kansas State University, Manhattan 66506, USA.
authors.forename: N V
authors.initials: NV
authors.lastname: Khramtsov
authors.forename: D S
authors.initials: DS
authors.lastname: Blunt
authors.forename: B A
authors.initials: BA
authors.lastname: Montelone
authors.forename: S J
authors.initials: SJ
authors.lastname: Upton
mesh_terms: D000106:Acetate-CoA Ligase / Q000737:chemistry* / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D003062:Codon / Q000737:chemistry; D017124:Conserved Sequence; D016785:Cryptosporidium parvum / Q000201:enzymology* / Q000235:genetics*; D016054:DNA, Protozoan / Q000032:analysis / Q000737:chemistry; D017125:Genes, Protozoan; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
chemical_list: D003062:Codon; D016054:DNA, Protozoan; D000106:Acetate-CoA Ligase