title: Selective loss of human leukocyte antigen class I allele expression in advanced renal cell carcinoma.
abstract: The expression of human leukocyte antigen (HLA) class I alleles was analyzed in 65 renal cell carcinomas using one-dimensional isoelectric focusing. Normal organ tissue and peripheral blood lymphocytes were used as controls. The patients were serologically typed using the standard microcytotoxicity test. Forty-two patients were staged as pT1 or pT2, and 23 patients had advanced tumor stages (pT3/pT4). In all cases the HLA-A,B phenotypes were confirmed using one-dimensional isoelectric focusing. The expression of HLA expression was reduced in two tumors [1 x HLA-A1(pT2); 1 x HLA-A28 (pT2)]. In three carcinomas the expression of HLA-A1 was lost. One tumor showed a combined loss of HLA-A2 and HLA-B38. These selective losses occurred in tumor stage pT3 (n = 1) or pT4 (n = 3; P = 0.013, Fisher's exact test). This leads to the conclusion that the loss of HLA expression is predominantly present in advanced tumor stages.
authors.affiliation: Department of Urology, Medical School Essen, Germany.
authors.forename: H J
authors.initials: HJ
authors.lastname: Luboldt
authors.forename: B S
authors.initials: BS
authors.lastname: Kubens
authors.forename: H
authors.initials: H
authors.lastname: Rübben
authors.forename: H
authors.initials: H
authors.lastname: Grosse-Wilde
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000483:Alleles; D002292:Carcinoma, Renal Cell / Q000235:genetics / Q000276:immunology* / Q000473:pathology / Q000601:surgery; D005260:Female; D015870:Gene Expression; D005805:Genes, MHC Class I; D014644:Genetic Variation; D015234:HLA-A Antigens / Q000032:analysis / Q000096:biosynthesis*; D015235:HLA-B Antigens / Q000032:analysis / Q000096:biosynthesis*; D006801:Humans; D007525:Isoelectric Focusing; D007680:Kidney Neoplasms / Q000235:genetics / Q000276:immunology* / Q000473:pathology / Q000601:surgery; D008214:Lymphocytes / Q000276:immunology; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D016133:Polymerase Chain Reaction
chemical_list: D015234:HLA-A Antigens; D015235:HLA-B Antigens