Chemical sympathectomy blocks androgen biosynthesis during prepuberty.
Twenty-one-day old male Wistar rats were injected subcutaneously with guanethidine (GUA) at doses of 5 and 10 mg kg-1 day-1 for 20 days. Animals were sacrificed by decapitation during the prepubertal (41 days of age) and early-pubertal (51 days of age) periods of sexual development. The testes were collected, frozen in liquid N2 and stored at -70 degrees C until determination of testicular progesterone (P), androstenedione (A) and testosterone (T). Higher levels of P (2.18 +/- 0.24 ng/g, control = 1.24 +/- 0.16 ng/g) associated with decreased levels of androgens (A = 0.26 +/- 0.06 ng/g and T = 2.05 +/- 0.19 ng/g; control = 1.86 +/- 0.76 ng/g and 8.48 +/- 1.16 ng/g, respectively) were observed in 10 mg GUA-treated rats of prepubertal age, while only P levels (3.12 +/- 0.51 ng/g, control = 1.73 +/- 0.27 ng/g) were increased in rats of early pubertal age. It is important to note that in 41-day old male rats both 5 and 10 mg were effective in decreasing testicular concentration of testosterone. These results suggest that the sympathetic innervation of the testis is involved in the modulation of androgen biosynthesis, acting through a selective step in the steroid biochemical pathway during the pubertal process and that under the conditions employed the blockage in androgen biosynthesis in the prepubertal stage of sexual maturation in dependent on the dose of GUA.
Rosa-e-Silva
A A
AA
Departamento de Fisiologia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Brasil.
Guimarães
M A
MA
Lamano-Carvalho
T L
TL
Kempinas
W G
WG
D000728:Androgens / Q000096:biosynthesis*; D000818:Animals; D006145:Guanethidine / Q000008:administration & dosage*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012741:Sexual Maturation / Q000187:drug effects / Q000502:physiology*; D013563:Sympathectomy, Chemical / Q000009:adverse effects*
D000728:Androgens; D006145:Guanethidine