Isolation and characterization of an auxin-inducible glutathione S-transferase gene of Arabidopsis thaliana.
Genes homologous to the auxin-inducible Nt103 glutathione S-transferase (GST) gene of tobacco, were isolated from a genomic library of Arabidopsis thaliana. We isolated a lambda clone containing an auxin-inducible gene, At103-1a, and part of a constitutively expressed gene, At103-1b. The coding regions of the Arabidopsis genes were highly homologous to each other and to the coding region of the tobacco gene but distinct from the GST genes that have been isolated from arabidopsis thusfar. Overexpression of a cDNA clone in Escherichia coli revealed that the AT103-1A protein had GST activity.
van der Kop
D A
DA
Institute of Molecular Plant Sciences, Leiden University, The Netherlands.
Schuyer
M
M
Scheres
B
B
van der Zaal
B J
BJ
Hooykaas
P J
PJ
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics*; D001483:Base Sequence; D004790:Enzyme Induction; D004926:Escherichia coli / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D015698:Genomic Library; D005982:Glutathione Transferase / Q000096:biosynthesis / Q000235:genetics*; D007210:Indoleacetic Acids / Q000494:pharmacology; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D018749:RNA, Plant / Q000032:analysis; D011994:Recombinant Proteins / Q000096:biosynthesis; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D007210:Indoleacetic Acids; D010940:Plant Proteins; D012333:RNA, Messenger; D018749:RNA, Plant; D011994:Recombinant Proteins; D005982:Glutathione Transferase