title: Activity of the mitochondrial multiple conductance channel is independent of the adenine nucleotide translocator.
abstract: The functional relationship between the adenine nucleotide translocator (ANT) and the mitochondrial multiple conductance channel (MCC) was investigated using patch-clamp techniques. MCC activity with the same conductance, ion selectivity, voltage dependence, and peptide sensitivity could be reconstituted from inner membrane fractions derived from mitochondria of ANT-deficient and wild-type Saccharomyces cerevisiae. In addition, the MCC activity of mouse kidney mitoplasts was unaffected by carboxyatractyloside, a known inhibitor of ANT and inducer of a permeability transition. These results suggest that MCC activity is independent of ANT.
authors.affiliation: Division of Molecular Medicine, Wadsworth Center, New York State Department of Health, Albany, New York, 12201 0509, USA.
authors.forename: T A
authors.initials: TA
authors.lastname: Lohret
authors.forename: R C
authors.initials: RC
authors.lastname: Murphy
authors.forename: T
authors.initials: T
authors.lastname: Drgoñ
authors.forename: K W
authors.initials: KW
authors.lastname: Kinnally
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001278:Atractyloside / Q000031:analogs & derivatives / Q000494:pharmacology; D003576:Electron Transport Complex IV / Q000737:chemistry; D004791:Enzyme Inhibitors / Q000494:pharmacology; D007425:Intracellular Membranes / Q000187:drug effects / Q000502:physiology*; D007473:Ion Channels / Q000187:drug effects / Q000502:physiology*; D007668:Kidney / Q000502:physiology; D007700:Kinetics; D008564:Membrane Potentials; D051379:Mice; D008928:Mitochondria / Q000502:physiology*; D000226:Mitochondrial ADP, ATP Translocases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D008969:Molecular Sequence Data; D018408:Patch-Clamp Techniques; D010455:Peptides / Q000138:chemical synthesis / Q000494:pharmacology*; D012441:Saccharomyces cerevisiae / Q000502:physiology
chemical_list: D004791:Enzyme Inhibitors; D007473:Ion Channels; D010455:Peptides; D001278:Atractyloside; D000226:Mitochondrial ADP, ATP Translocases; D003576:Electron Transport Complex IV; C003853:carboxyatractyloside