Genomic structure and promoter analysis of the human obese gene.
The human gene encoding the homolog of the mouse obese (ob) gene was isolated and partially characterized. The human ob gene consists of three exons and two introns and spans about 18 kilobase pairs (kb), encoding a 3.5-kb cDNA. A 3-kb 5'-flanking region of the gene was cloned and transient transfection assay with luciferase reporter confirmed the promoter activity in differentiated F442-A adipocytes. Potential regulatory elements are discussed in this report.
Gong
D W
DW
Molecular and Cellular Endocrinology Branch and Phoenix Epidemiology and Clinical Research Branch, NIDDK, National Institutes of Health, Bethesda, Maryland 20892, USA.
Bi
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S
Pratley
R E
RE
Weintraub
B D
BD
D017667:Adipocytes / Q000378:metabolism; D000273:Adipose Tissue / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D018076:DNA, Complementary; D006801:Humans; D020738:Leptin; D008156:Luciferases / Q000032:analysis / Q000096:biosynthesis; D051379:Mice; D008969:Molecular Sequence Data; D009765:Obesity / Q000235:genetics*; D011401:Promoter Regions, Genetic; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000378:metabolism; D011994:Recombinant Proteins / Q000032:analysis / Q000096:biosynthesis; D012045:Regulatory Sequences, Nucleic Acid; D014162:Transfection
D018076:DNA, Complementary; D020738:Leptin; D011506:Proteins; D012333:RNA, Messenger; D011994:Recombinant Proteins; D008156:Luciferases