Construction of a chromosome specific library of human MARs and mapping of matrix attachment regions on human chromosome 19.
Using a novel procedure a representative human chromosome 19-specific library was constructed of short sequences, which bind preferentially to the nuclear matrix (matrix attachment regions, or MARs). Judging by 20 clones sequenced so far, the library contains > 50% of human inserts, about 90% of which are matrix-binding by the in vitro test. Computer analysis of sequences of eight human MARs did not reveal any significant homologies with the EMBL Nucleotide Data Base entries as well as between MARs themselves. Eight MARs were assigned to individual positions on the chromosome 19 physical map. The library constructed can serve as a good source of MAR sequences for comparative analysis and classification and for further chromosome mapping of MARs as well.
Nikolaev
L G
LG
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
Tsevegiyn
T
T
Akopov
S B
SB
Ashworth
L K
LK
Sverdlov
E D
ED
D001483:Base Sequence; D001665:Binding Sites; D002888:Chromosomes, Human, Pair 19; D017931:DNA Primers / Q000737:chemistry; D004268:DNA-Binding Proteins / Q000378:metabolism*; D015698:Genomic Library; D006801:Humans; D008969:Molecular Sequence Data; D015530:Nuclear Matrix / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D014407:Tumor Cells, Cultured
D017931:DNA Primers; D004268:DNA-Binding Proteins