Immunoprophylaxis of allergen-induced immunoglobulin E synthesis and airway hyperresponsiveness in vivo by genetic immunization.
The efficacy of an "allergen-gene immunization" protocol in altering allergic response was examined. Intramuscular injection of rats with a plasmid DNA encoding a house dust mite allergen into the muscle results in its long-term expression and the induction of specific immune responses. Significantly, this approach prevents the induction of immunoglobulin E synthesis, histamine release in bronchoalveolar fluids, and airway hyperresponsiveness in rats challenged with aerosolized allergen. Furthermore, this suppression is persistent and can be transferred into naive rats by CD8+ T cells from gene-immunized rats. These findings suggest that allergen-gene immunization is effective in modulating allergic responses, and may provide a novel therapeutic approach for allergic diseases.
Hsu
C H
CH
Graduate Institute of Microbiology, College of Medicine, National Taiwan University, Taipei, Republic of China.
Chua
K Y
KY
Tao
M H
MH
Lai
Y L
YL
Wu
H D
HD
Huang
S K
SK
Hsieh
K H
KH
D000818:Animals; D039741:Antigens, Dermatophagoides; D001992:Bronchoalveolar Lavage Fluid; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology / Q000637:transplantation; D004274:DNA, Recombinant / Q000627:therapeutic use*; D006023:Glycoproteins / Q000235:genetics / Q000276:immunology / Q000627:therapeutic use*; D006632:Histamine / Q000378:metabolism; D007073:Immunoglobulin E / Q000096:biosynthesis*; D007150:Immunohistochemistry; D008297:Male; D051381:Rats; D012130:Respiratory Hypersensitivity / Q000276:immunology / Q000517:prevention & control*; D014611:Vaccination
D039741:Antigens, Dermatophagoides; D004274:DNA, Recombinant; D006023:Glycoproteins; D007073:Immunoglobulin E; D006632:Histamine