Morphology of glycerol-preserved human cadaver skin.
Donor allograft skin preserved in 85 per cent glycerol has been used successfully as a temporary coverage for large burn wounds. The glycerol preservation is a method with low costs and has practical advantages such as antibacterial and virucidal effects. This report shows that the glycerol treatment did not affect the fundamental structural integrity of the skin. Intact keratinocytes and Langerhans cells with their characteristic Birbeck granules were still present in the glycerol-treated skin. After treatment with glycerol, the cells in the prepared epidermal cell suspensions were non-viable. MHC class II positive and CD1a positive cells could still be identified in situ and in the suspension.
Richters
C D
CD
Department of Cell Biology and Immunology, Faculty of Medicine, Vrije Universiteit, Amsterdam, The Netherlands.
Hoekstra
M J
MJ
van Baare
J
J
du Pont
J S
JS
Kamperdijk
E W
EW
D003451:Cryoprotective Agents / Q000494:pharmacology*; D005990:Glycerol / Q000494:pharmacology*; D006801:Humans; D016130:Immunophenotyping; D008854:Microscopy, Electron; D012867:Skin / Q000166:cytology / Q000187:drug effects* / Q000648:ultrastructure; D014021:Tissue Preservation
D003451:Cryoprotective Agents; D005990:Glycerol