title: Molecular basis of the soluble and membrane-bound forms of the murine leukemia inhibitory factor receptor alpha-chain. Expression in normal, gestating, and leukemia inhibitory factor nullizygous mice.
abstract: The murine leukemia inhibitory factor receptor alpha-chain (mLIFR) exists in a membrane-bound and a soluble form. The two major classes of mRNA transcript correspond to either the soluble or membrane-bound form of the mLIFR. In this study we have identified a complex and heterogeneous pattern of expression of mRNA transcripts for this receptor in normal mouse tissues and cell lines. In order to understand the molecular basis of these transcripts, genomic clones encompassing the region of divergence from the soluble to the membrane-bound form of the receptor were isolated. cDNAs encoding the membrane-bound form of the mLIFR were generated by an alternative splicing event where an exon that is specific to the soluble mLIFR was skipped. The membrane-bound form of the mLIFR was heterogeneously polyadenylated with at least five different sites of polyadenylation. The mRNA transcript encoding the soluble form of the mLIFR contained a region highly homologous to a murine B2 repetitive element, thus providing a possible explanation for the genesis of this transcript. The different forms of the mLIFR were analyzed in a wide range of mouse tissues in pseudopregnant mice and in mice at various stages of pregnancy. Only liver, placenta, and uterus showed an increase in the levels of mLIFR mRNA expression during pregnancy, indicating an important role for the LIFR in this process. However, somewhat surprisingly, there was no detectable difference in mLIFR mRNA levels or levels of soluble protein in leukemia inhibitory factor nullizygous mice when compared with normal mice.
authors.affiliation: Walter and Eliza Hall Institute of Medical Research, P. O. Royal Melbourne Hospital, Victoria 3050, Australia.
authors.forename: C M
authors.initials: CM
authors.lastname: Owczarek
authors.forename: M J
authors.initials: MJ
authors.lastname: Layton
authors.forename: L G
authors.initials: LG
authors.lastname: Robb
authors.forename: N A
authors.initials: NA
authors.lastname: Nicola
authors.forename: C G
authors.initials: CG
authors.lastname: Begley
mesh_terms: D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D001921:Brain / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D004247:DNA / Q000302:isolation & purification; D017931:DNA Primers; D005091:Exons; D005260:Female; D015870:Gene Expression; D006131:Growth Inhibitors / Q000378:metabolism*; D006801:Humans; D015850:Interleukin-6; D007438:Introns; D053605:Leukemia Inhibitory Factor; D053680:Leukemia Inhibitory Factor Receptor alpha Subunit; D008099:Liver / Q000378:metabolism; D008222:Lymphokines / Q000378:metabolism*; D046911:Macromolecular Substances; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D010920:Placenta / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000378:metabolism*; D018809:Proliferating Cell Nuclear Antigen / Q000235:genetics; D011555:Pseudopregnancy; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D018121:Receptors, Cytokine / Q000096:biosynthesis* / Q000235:genetics*; D053606:Receptors, OSM-LIF; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D013234:Stem Cells; D014158:Transcription, Genetic; D014599:Uterus / Q000378:metabolism
chemical_list: D017931:DNA Primers; D006131:Growth Inhibitors; D015850:Interleukin-6; C507837:LIF protein, human; C507840:LIFR protein, human; D053605:Leukemia Inhibitory Factor; D053680:Leukemia Inhibitory Factor Receptor alpha Subunit; C507838:Lif protein, mouse; C507841:Lifr protein, mouse; D008222:Lymphokines; D046911:Macromolecular Substances; D018809:Proliferating Cell Nuclear Antigen; D012333:RNA, Messenger; D018121:Receptors, Cytokine; D053606:Receptors, OSM-LIF; D004247:DNA