Characterization of a chicken monoclonal antibody that recognizes the apical complex of Eimeria acervulina sporozoites and partially inhibits sporozoite invasion of CD8+ T lymphocytes in vitro.
A stable chicken hybridoma secreting a monoclonal antibody (mAb) that detects the apical complex of Eimeria acervulina sporozoites has been developed by fusing a thymidine kinase (TK)-deficient chicken myeloma with spleen cells from chickens immunized with sporozoite antigen. The mAb, designated as 6D-12-G10, recognized the apical complex of a sporozoite of 20-21 kDa molecular mass on western blots. Immunoelectron microscopic examination revealed that mAb 6D-12-G10 stained the conoid antigen. Furthermore, mAb 6D-12-G10 inhibited the invasion of sporozoites into CD8+ T cells in vitro. These results suggest that the conoid may play an important role in the recognition and invasion of host cells by Eimeria sporozoites.
Sasai
K
K
Parasite Immunobiology Laboratory, United States Department of Agriculture, ARS Beltsville, Maryland 20705, USA.
Lillehoj
H S
HS
Matsuda
H
H
Wergin
W P
WP
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology*; D000918:Antibody Specificity; D000953:Antigens, Protozoan / Q000737:chemistry / Q000276:immunology; D015153:Blotting, Western; D018414:CD8-Positive T-Lymphocytes / Q000469:parasitology*; D002478:Cells, Cultured; D002645:Chickens; D004306:Dose-Response Relationship, Immunologic; D004539:Eimeria / Q000276:immunology* / Q000648:ultrastructure; D019084:Fluorescent Antibody Technique, Indirect; D006825:Hybridomas; D016253:Microscopy, Immunoelectron; D008970:Molecular Weight; D009101:Multiple Myeloma; D013154:Spleen / Q000166:cytology; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan