A yeast artificial chromosome-based map of the region of chromosome 20 containing the diabetes-susceptibility gene, MODY1, and a myeloid leukemia related gene.
We have generated a physical map of human chromosome bands 20q11.2-20q13.1, a region containing a gene involved in the development of one form of early-onset, non-insulin-dependent diabetes mellitus, MODY1, as well as a putative myeloid tumor suppressor gene. The yeast artificial chromosome contig consists of 71 clones onto which 71 markers, including 20 genes, 5 expressed sequence tags, 32 simple tandem repeat DNA polymorphisms, and 14 sequence-tagged sites have been ordered. This region spans about 18 Mb, which represents about 40% of the physical length of 20q. Using this physical map, we have refined the location of MODY1 to a 13-centimorgan interval (approximately equal to 7 Mb) between D20S169 and D20S176. The myeloid tumor suppressor gene was localized to an 18-centimorgan interval (approximately equal to 13 Mb) between RPN2 and D20S17. This physical map will facilitate the isolation of MODY1 and the myeloid tumor suppressor gene.
Stoffel
M
M
Howard Hughes Medical Institute, University of Chicago, IL 60637, USA.
Le Beau
M M
MM
Espinosa
R
R
Bohlander
S F
SF
Le Paslier
D
D
Cohen
D
D
Xiang
K S
KS
Cox
N J
NJ
Fajans
S S
SS
Bell
G I
GI
D001483:Base Sequence; D002874:Chromosome Mapping; D018244:Chromosomes, Artificial, Yeast; D002890:Chromosomes, Human, Pair 20; D004247:DNA / Q000302:isolation & purification; D017931:DNA Primers; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics*; D004198:Disease Susceptibility; D004587:Electrophoresis, Agar Gel; D005260:Female; D016147:Genes, Tumor Suppressor; D005819:Genetic Markers; D006239:Haplotypes; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007951:Leukemia, Myeloid / Q000235:genetics*; D008297:Male; D008969:Molecular Sequence Data; D010375:Pedigree; D011110:Polymorphism, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D016324:Sequence Tagged Sites
D017931:DNA Primers; D005819:Genetic Markers; D004247:DNA