Morphological analysis of saphenous vein used for aortocoronary graft.
Histological, histochemical, immunohistochemical and ultrastructural analyses of 120 saphenous veins (of 120 patients) prepared for aortocoronary graft, revealed in 57 cases lesser or larger morphological changes, such as endothelial damage, hyperplasia of t. intima, or t. media, and fibrosis of t. adventitia that could be the result of postoperative complications (thrombosis of the graft lumen, or its occlusion due to progressive hyperplasia of t. intima). The analyses have proved significantly increased number of smooth muscle cells and fibroblasts in t. intima of the damaged veins in relation to t. media, contrary to the findings in normal veins, and it was suggested that these smooth muscle cells in the damaged veins migrated from t. media to t. intima causing hyperplasia, as was confirmed by histochemical, immunohistochemical and ultrastructural findings. Since in 57 of 120 analyzed veins morphological changes were found which developed before the implantation of vein and which could compromise the vein function as the aortocoronary graft and cause severe postoperative complications, forming of vein banks is recommended from the chosen donors to avoid vein implantation with the already existent morphological changes, that could be proven by their immediate histological examination.
Tatić
V
V
Military Medical Academy, Institute for Pathology and Forensic Medicine, Belgrade.
Spasić
P
P
Kanjuh
V
V
Todorić
M
M
D000328:Adult; D000368:Aged; D001026:Coronary Artery Bypass; D005169:Factor VIII / Q000032:analysis; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007381:Intermediate Filament Proteins / Q000032:analysis; D008297:Male; D008875:Middle Aged; D012501:Saphenous Vein / Q000737:chemistry / Q000473:pathology* / Q000637:transplantation / Q000648:ultrastructure
D007381:Intermediate Filament Proteins; D005169:Factor VIII