Lack of MHC class I complex expression has no effect on spread and control of cytomegalovirus infection in vivo.
It has been claimed that MHC class I proteins serve as receptors for murine cytomegalovirus (MCMV) and that this interaction is the most important mechanism for virus entry in most cells. This claim is based on the observation that the MHC haplotype contributes to the susceptibility to cytomegalovirus (CMV) infection in vivo. Results from in vitro studies support the concept that stable expression of correctly folded MHC class I molecules contributes to infection, since the individual properties of MHC class I alleles, the availability of beta 2-microglobulin (beta 2m) and also the degree of peptide charging of the MHC class I heavy chain beta 2m heterodimers determined the infection phenotype of cell lines. To assess the biological relevance of proper MHC class I expression we investigated CMV infection in beta 2m-deficient mice which fail to express ternary MHC class I complexes and lack peripheral CD8+ T lymphocytes. We found that organ virus titres and virus clearance kinetics were not altered in beta 2m mutant mice. In addition, there was no indication of diminished virus propagation in beta 2m-/- embryonic fibroblasts. beta 2m-/- mice suffered from the lack of CD8+ T lymphocytes that was partially compensated for by the function of CD4+ T lymphocytes. An organ-specific anti-virus function of natural killer (NK) cells was observed, independent from the beta 2m deletion. The immune control unique for salivary gland infection was maintained. From the data presented here, we confirm the role of MHC class I molecules in the immune surveillance of CMV infection but question the biological impact of correct MHC class I complexes for productive infection.
Polić
B
B
Department of Physiology and Immunology, Medical Faculty, University of Rijeka, Croatia.
Jonjić
S
S
Pavić
I
I
Crnković
I
I
Zorica
I
I
Hengel
H
H
Lucin
P
P
Koszinowski
U H
UH
D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000502:physiology; D003586:Cytomegalovirus Infections / Q000276:immunology*; D015395:Histocompatibility Antigens Class I / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D051381:Rats; D001613:beta 2-Microglobulin / Q000172:deficiency
D015395:Histocompatibility Antigens Class I; D001613:beta 2-Microglobulin