title: Intestinal expression of apolipoprotein A-IV and C-III is coordinately regulated by dietary lipid in newborn swine.
abstract: The purpose of this study was to determine the effects of dietary fatty acids on intestinal apolipoprotein (apo) A-IV and C-III expression in the newborn piglet. Two-day-old female piglets received one of three isocaloric formulas containing medium-chain triglycerides (TG) (MCT), intermediate-chain saturated TG (ICST), or long-chain polyunsaturated TG (LCPUT) by continuous duodenal infusion for 24 hrs. Control groups received a low-TG elemental formula infusion (LTG group) and a high-TG long-chain unsaturated lipid emulsion infusion (HTG group). Jejunal synthesis of both apo A-IV and C-III was increased by all infusions compared to the LTG group. Apo A-IV and C-III mRNA levels paralleled synthesis levels except for apo C-III with the MCT diet, where regulation appeared to occur at the translational level. Among all groups, apo A-IV synthesis and mRNA abundance were linearly correlated with that of apo C-III.
authors.affiliation: Department of Pediatrics, University of Arkansas for Medical Sciences, Arkansas Children's Hospital Research Institute, Little Rock 72202, USA.
authors.forename: D D
authors.initials: DD
authors.lastname: Black
authors.forename: H
authors.initials: H
authors.lastname: Wang
authors.forename: F
authors.initials: F
authors.lastname: Hunter
authors.forename: R
authors.initials: R
authors.lastname: Zhan
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D053305:Apolipoprotein C-III; D001054:Apolipoproteins A / Q000235:genetics*; D001056:Apolipoproteins C / Q000235:genetics*; D004041:Dietary Fats / Q000494:pharmacology*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects*; D007583:Jejunum / Q000378:metabolism*; D013552:Swine
chemical_list: D053305:Apolipoprotein C-III; D001054:Apolipoproteins A; D001056:Apolipoproteins C; D004041:Dietary Fats; C022205:apolipoprotein A-IV