title: The chromatin of the Saccharomyces cerevisiae centromere shows cell-type specific changes.
abstract: We have analysed the centromeric chromatin from chromosome XIV of Saccharomyces cerevisiae at different stages of mitosis with the help of mutants of the cell division cycle. The pattern of centromeric chromatin in cells arrested using cdc20-1, tub2-401 and cdc15-1 alleles was indistinguishable from that of vegetatively growing cells, indicating that the centromeric complex is constitutively present during mitosis and possibly throughout the entire cell cycle. In contrast chromatin isolated from G0 cells and spores exhibited distinct differences in centromeric chromatin probably due to structural rearrangements of the centromeric complex. In particular the alterations found in spores are indicative of an inactive centromeric complex. The differences in centromeric chromatin in spores do not reflect a general reorganisation of the chromatin in this cell type, as the chromatin structure of the PHO3/PHO5 locus in spores was found to be identical to that in vegetative cells under repressed conditions. Thus the structural analysis of the centromere in different cell types provides evidence about the requirement of CEN DNA/protein complexes in different cell types and in different stages of the cell cycle.
authors.affiliation: Institut für Mikrobiologie und Molekularbiologie, Justus-Liebig-Universität, Frankfurter Strasse 107, D-35392 Giessen, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Wilmen
authors.forename: J H
authors.initials: JH
authors.lastname: Hegemann
mesh_terms: D000135:Acid Phosphatase; D002352:Carrier Proteins / Q000235:genetics; D002453:Cell Cycle / Q000235:genetics; D002503:Centromere / Q000737:chemistry*; D002843:Chromatin / Q000737:chemistry*; D015825:Chromosomes, Fungal / Q000737:chemistry*; D004271:DNA, Fungal / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics; D026901:Membrane Transport Proteins / Q000235:genetics; D008870:Microtubules / Q000378:metabolism; D008938:Mitosis; D028061:Phosphate Transport Proteins; D012441:Saccharomyces cerevisiae / Q000737:chemistry* / Q000235:genetics / Q000254:growth & development; D029701:Saccharomyces cerevisiae Proteins; D013172:Spores, Fungal
chemical_list: D002352:Carrier Proteins; D002843:Chromatin; D004271:DNA, Fungal; D005656:Fungal Proteins; D026901:Membrane Transport Proteins; D028061:Phosphate Transport Proteins; D029701:Saccharomyces cerevisiae Proteins; C026989:thiamine-binding protein; C055831:phosphate permease; D000135:Acid Phosphatase; C488913:PHO3 protein, S cerevisiae; C488916:PHO5 protein, S cerevisiae