Elevation of internal 6-methyladenine mRNA methyltransferase activity after cellular transformation.
A comparison of internal 6-methyladenine mRNA methyltransferase activity in a variety of cell types demonstrated an 8-15-fold increase as a result of cellular transformation. Utilizing adenovirus transformed rat embryo cells, it was found that the increase in methyltransferase activity was concomitant with or occurred rapidly after transformation. An 80-fold increase in activity was observed in the cells isolated from the transformed foci and remained elevated through subsequent passages. The relationship between methyltransferase activity and tumor formation was also investigated. High level expression of the avian ski oncogene in mouse L cells causes a reversion of the transformed phenotype to a non-transformed state, and resulted in a 47% reduction in the specific activity of the methyltransferase as compared with mock transfected cells.
Tuck
M T
MT
Department of Chemistry, Clippinger Laboratories, Ohio University, Athens 45701, USA.
James
C B
CB
Kelder
B
B
Kopchick
J J
JJ
D000256:Adenoviridae; D000818:Animals; D001717:Birds; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D006224:Cricetinae; D006367:HeLa Cells; D006801:Humans; D007700:Kinetics; D007739:L Cells; D008780:Methyltransferases / Q000378:metabolism*; D051379:Mice; D009687:Nuclear Proteins / Q000096:biosynthesis; D009857:Oncogenes; D051381:Rats; D015682:Retroviridae Proteins, Oncogenic / Q000096:biosynthesis; D013762:Tetrahydrofolate Dehydrogenase / Q000096:biosynthesis; D014157:Transcription Factors; D014158:Transcription, Genetic
D009687:Nuclear Proteins; D015682:Retroviridae Proteins, Oncogenic; D014157:Transcription Factors; C070358:v-ski protein, Avian retrovirus; D013762:Tetrahydrofolate Dehydrogenase; C065638:6-methyladenine mRNA methyltransferase; D008780:Methyltransferases