title: Induction of apoptosis as well as necrosis by hypoxia and predominant prevention of apoptosis by Bcl-2 and Bcl-XL.
abstract: The molecular mechanism of cell death due to hypoxia has not been elucidated. Our recent observations that overexpression of the anti-apoptotic proto-oncogene bcl-2 and a bcl-2-related gene, bcl-x, prevents hypoxic cell death suggest that hypoxia induces apoptosis. Using electron microscopy and confocal and nonconfocal fluorescence microscopy, we show here that hypoxia does, in fact, induce both necrosis and apoptosis, and that the proportion of these two modes is highly dependent on the cell type. Overexpression of Bcl-2 or Bcl-Xl blocks hypoxia-induced apoptosis in a dose-dependent manner.
authors.affiliation: First Department of Surgery, Osaka University Medical School, Suita, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Shimizu
authors.forename: Y
authors.initials: Y
authors.lastname: Eguchi
authors.forename: W
authors.initials: W
authors.lastname: Kamiike
authors.forename: Y
authors.initials: Y
authors.lastname: Itoh
authors.forename: J
authors.initials: J
authors.lastname: Hasegawa
authors.forename: K
authors.initials: K
authors.lastname: Yamabe
authors.forename: Y
authors.initials: Y
authors.lastname: Otsuki
authors.forename: H
authors.initials: H
authors.lastname: Matsuda
authors.forename: Y
authors.initials: Y
authors.lastname: Tsujimoto
mesh_terms: D000818:Animals; D017209:Apoptosis; D015687:Cell Hypoxia; D018014:Gene Transfer Techniques; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D009336:Necrosis; D016716:PC12 Cells / Q000378:metabolism / Q000473:pathology; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis* / Q000235:genetics; D019253:Proto-Oncogene Proteins c-bcl-2; D051381:Rats; D051020:bcl-X Protein
chemical_list: C494813:Bcl2l1 protein, rat; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D051020:bcl-X Protein