DNA affinity to biological membranes is enhanced due to complexation with hydrophobized polycation.
The interaction of negatively charged liquid phosphatidylcholine/cardiolipin liposomes with water-soluble negatively charged DNA/cetylpyridinium bromide and DNA/poly(N-alkyl-4-vinylpyridinium bromide) complexes was studied. It is shown that the DNA/cetylpyridinium bromide complex while interacting with the liposomes is destroyed, so that the cetylpyridinium cation is incorporated into the liposomal membrane and DNA remains in the solution. The DNA/poly-(N-ethyl-4-vinylpyridinium bromide) complex does not interact at all with the liposomes. On the contrary, the complex of DNA with the poly(vinylpyridinium) cation carrying a small amount of N-cetyl groups is adsorbed on the membrane as a whole. The data obtained indicate that complexation of DNA with hydrophobized polycations can be used for enhancing DNA affinity to biological membranes.
Yaroslavov
A A
AA
Department of Polymer Sciences, Faculty of Chemistry, M.V. Lomonosov Moscow State University, Russian Federation.
Sukhishvili
S A
SA
Obolsky
O L
OL
Yaroslavova
E G
EG
Kabanov
A V
AV
Kabanov
V A
VA
D002308:Cardiolipins / Q000378:metabolism; D002412:Cations / Q000378:metabolism*; D002594:Cetylpyridinium / Q000378:metabolism*; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004247:DNA / Q000378:metabolism*; D008081:Liposomes / Q000378:metabolism*; D008563:Membrane Lipids / Q000378:metabolism; D010713:Phosphatidylcholines / Q000378:metabolism; D011145:Polyvinyls / Q000378:metabolism*
D002308:Cardiolipins; D002412:Cations; D008081:Liposomes; D008563:Membrane Lipids; D010713:Phosphatidylcholines; D011145:Polyvinyls; C027968:poly-4-vinyl-N-ethylpyridine; D004247:DNA; D002594:Cetylpyridinium