Analysis of 94 kb of the chlorella virus PBCV-1 330-kb genome: map positions 88 to 182.
Analysis of 94 kb of DNA, located between map positions 88 and 182 kb in the 330-kb chlorella virus PBCV-1 genome, revealed 195 open reading frames (ORFs) 65 codons or longer. One hundred and five of the 195 ORFs were considered major ORFs. Twenty-six of the 105 major ORFs resembled genes in the databases including three chitinases, a chitosanase, three serine/threonine protein kinases, two additional protein kinases, a tyrosine protein phosphatase, two ankyrins, an ornithine decarboxylase, a copper/zinc-superoxide dismutase, a proliferating cell nuclear antigen, a DNA polymerase, a fibronectin-binding protein, the yeast Ski2 protein, an adenine DNA methyltransferase and its corresponding DNA site-specific endonuclease, and an amidase. The genes for the 105 major ORFs were evenly distributed along the genome and, except for one noncoding 1788-nucleotide stretch, the genes were close together. Unexpectedly, a 900-bp region in the 1788-bp noncoding sequence resembled a CpG island.
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Plum Island Animal Disease Center, ARS, USDA, NAA, Greenport, New York 11944-0848, USA.
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Kutish
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Rock
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Van Etten
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D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002708:Chlorella / Q000821:virology; D002874:Chromosome Mapping; D016208:Databases, Factual; D016679:Genome, Viral; D006801:Humans; D007438:Introns; D008969:Molecular Sequence Data; D005810:Multigene Family; D017862:Phycodnaviridae / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D014764:Viral Proteins / Q000235:genetics
D014764:Viral Proteins