Enhancing effects of epidermal growth factor on human squamous cell carcinoma motility and matrix degradation but not growth.
In order to ascertain the effects of epidermal growth factor (EGF) on human cancer invasion abilities, three cell lines of human oral squamous cell carcinoma were studied using a phagokinetic track assay and zymography. EGF (1-100 ng/ml) was found to inhibit the growth but enhance the random motility of all three cell lines in a concentration-dependent fashion. Exposure to EGF, dose-dependently, led to an increased production of urokinase-type plasminogen activator and M(r) 92 kD matrix metalloproteinase by the same cells. These results strongly suggest that EGF may promote human squamous cell carcinoma invasion and metastasis.
Shibata
T
T
Department of Oral Surgery, Health Sciences University of Hokkaido, Japan.
Kawano
T
T
Nagayasu
H
H
Okumura
K
K
Arisue
M
M
Hamada
J
J
Takeichi
N
N
Hosokawa
M
M
D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D002455:Cell Division; D002465:Cell Movement / Q000187:drug effects; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D005109:Extracellular Matrix / Q000378:metabolism; D006801:Humans; D008666:Metalloendopeptidases / Q000378:metabolism; D014407:Tumor Cells, Cultured; D014568:Urokinase-Type Plasminogen Activator / Q000378:metabolism
D004815:Epidermal Growth Factor; D014568:Urokinase-Type Plasminogen Activator; D008666:Metalloendopeptidases