title: The Tie receptor tyrosine kinase is expressed by human hematopoietic progenitor cells and by a subset of megakaryocytic cells.
abstract: Growth factor receptors in human hematopoietic progenitor cells have become the focus of intense interest, because they may provide tools for the monitoring, enrichment, and expansion of stem cells. We have shown earlier that the Tie receptor tyrosine kinase is expressed in erythroid and megakaryoblastic human leukemia cell lines, in the blood islands of the yolk sac, and in endothelial cells starting from day 8.0 of mouse development. Here, the expression of Tie was studied in human hematopoietic cells of various sources. Peripheral blood mononuclear cells were Tie-. However, a large fraction of CD34+ cells from umbilical cord blood (UCB) and bone marrow (BM) expressed tie protein and mRNA. On average, 64% of the fluorescence-activated cell sorting-gated UCB CD34+ cells including CD38- cells and a fraction of cells expressing low levels of c-Kit were Tie+. Also, 30% to 60% of BM CD34+ cells were Tie+, including most of the BM CD34+CD38-, CD34+Thy-1+, and CD34+HLA-DR- cells. Under culture conditions allowing myeloid, erythroid, and/or megakaryocytic differentiation, purified UCB CD34+ cells lost Tie mRNA and protein expression concomitantly with that of CD34; however, a significant fraction of cells expressed Tie during megakaryocytic differentiation. These data suggest that, in humans, the Tie receptor and presumably its ligand may function at an early stage of hematopoietic cell differentiation.
authors.affiliation: Laboratoire de biologie molécularie et cellulaire des facteurs de croissance, UPR 9044/CNRS, Villejuif, France.
authors.forename: P
authors.initials: P
authors.lastname: Batard
authors.forename: P
authors.initials: P
authors.lastname: Sansilvestri
authors.forename: C
authors.initials: C
authors.lastname: Scheinecker
authors.forename: W
authors.initials: W
authors.lastname: Knapp
authors.forename: N
authors.initials: N
authors.lastname: Debili
authors.forename: W
authors.initials: W
authors.lastname: Vainchenker
authors.forename: H J
authors.initials: HJ
authors.lastname: Bühring
authors.forename: M N
authors.initials: MN
authors.lastname: Monier
authors.forename: E
authors.initials: E
authors.lastname: Kukk
authors.forename: J
authors.initials: J
authors.lastname: Partanen
authors.forename: M T
authors.initials: MT
authors.lastname: Matikainen
authors.forename: R
authors.initials: R
authors.lastname: Alitalo
authors.forename: J
authors.initials: J
authors.lastname: Hatzfeld
authors.forename: K
authors.initials: K
authors.lastname: Alitalo
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D018952:Antigens, CD34; D001854:Bone Marrow Cells; D002454:Cell Differentiation; D002461:Cell Line, Transformed; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D017077:Culture Media, Conditioned; D004730:Endothelium, Vascular / Q000201:enzymology; D004790:Enzyme Induction; D005312:Fetal Blood / Q000166:cytology; D006412:Hematopoietic Stem Cells / Q000201:enzymology*; D006801:Humans; D007938:Leukemia / Q000201:enzymology / Q000473:pathology; D017493:Leukocyte Common Antigens; D008533:Megakaryocytes / Q000737:chemistry / Q000201:enzymology*; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000235:genetics; D019039:Platelet Glycoprotein GPIIb-IIIa Complex; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020794:Receptor Protein-Tyrosine Kinases / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D011956:Receptors, Cell Surface / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D042764:Receptors, TIE; D011994:Recombinant Proteins / Q000494:pharmacology; D013926:Thrombopoietin / Q000494:pharmacology; D014407:Tumor Cells, Cultured
chemical_list: D000911:Antibodies, Monoclonal; D018952:Antigens, CD34; D017077:Culture Media, Conditioned; D009363:Neoplasm Proteins; D019039:Platelet Glycoprotein GPIIb-IIIa Complex; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D011994:Recombinant Proteins; D013926:Thrombopoietin; D020794:Receptor Protein-Tyrosine Kinases; D042764:Receptors, TIE; D017493:Leukocyte Common Antigens