title: The x-ray crystal structure of phosphomannose isomerase from Candida albicans at 1.7 angstrom resolution.
abstract: Phosphomannose isomerase (PMI) catalyses the reversible isomerization of fructose-6-phosphate (F6P) and mannose-6-phosphate (M6P). Absence of PMI activity in yeasts causes cell lysis and thus the enzyme is a potential target for inhibition and may be a route to antifungal drugs. The 1.7 A crystal structure of PMI from Candida albicans shows that the enzyme has three distinct domains. The active site lies in the central domain, contains a single essential zinc atom, and forms a deep, open cavity of suitable dimensions to contain M6P or F6P The central domain is flanked by a helical domain on one side and a jelly-roll like domain on the other.
authors.affiliation: Glaxo Wellcome Research and Development, Department of Biomolecular Structure, Stevenage, UK.
authors.forename: A
authors.initials: A
authors.lastname: Cleasby
authors.forename: A
authors.initials: A
authors.lastname: Wonacott
authors.forename: T
authors.initials: T
authors.lastname: Skarzynski
authors.forename: R E
authors.initials: RE
authors.lastname: Hubbard
authors.forename: G J
authors.initials: GJ
authors.lastname: Davies
authors.forename: A E
authors.initials: AE
authors.lastname: Proudfoot
authors.forename: A R
authors.initials: AR
authors.lastname: Bernard
authors.forename: M A
authors.initials: MA
authors.lastname: Payton
authors.forename: T N
authors.initials: TN
authors.lastname: Wells
mesh_terms: D001665:Binding Sites; D002176:Candida albicans / Q000201:enzymology* / Q000235:genetics; D003198:Computer Simulation; D018360:Crystallography, X-Ray; D008359:Mannose-6-Phosphate Isomerase / Q000737:chemistry* / Q000235:genetics; D008667:Metalloproteins / Q000737:chemistry*; D008958:Models, Molecular; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D011994:Recombinant Proteins / Q000737:chemistry; D013045:Species Specificity; D015032:Zinc / Q000737:chemistry*
chemical_list: D008667:Metalloproteins; D011994:Recombinant Proteins; D008359:Mannose-6-Phosphate Isomerase; D015032:Zinc