title: Structural and immunochemical characterization of the type VIII group B Streptococcus capsular polysaccharide.
abstract: The type VIII capsular polysaccharide has been isolated and purified from a newly described strain of group B Streptococcus which is a leading cause of sepsis and neonatal meningitis in Japan. The polysaccharide contains D-glucose, D-galactose, L-rhamnose, and sialic acid in the molar ratio 1:1:1:1. By means of high resolution 1H nuclear magnetic resonance (1H NMR), 13C NMR, and homo- and heterocorrelated NMR, the repeating unit structure of the type VIII polysaccharide was delineated as the following, [formula: see text] Enzymatic studies established this polysaccharide as the first from which sialic acid, linked to a branched beta-D-galactopyranosyl residue, is known to be removed by bacterial neuraminidase.
authors.affiliation: Institute for Biological Sciences, National Research Council of Canada, Ottawa, Ontario, K1A 0R6, Canada.
authors.forename: G
authors.initials: G
authors.lastname: Kogan
authors.forename: D
authors.initials: D
authors.lastname: Uhrín
authors.forename: J R
authors.initials: JR
authors.lastname: Brisson
authors.forename: L C
authors.initials: LC
authors.lastname: Paoletti
authors.forename: A E
authors.initials: AE
authors.lastname: Blodgett
authors.forename: D L
authors.initials: DL
authors.lastname: Kasper
authors.forename: H J
authors.initials: HJ
authors.lastname: Jennings
mesh_terms: D000942:Antigens, Bacterial / Q000737:chemistry*; D016667:Bacterial Capsules / Q000737:chemistry* / Q000276:immunology*; D002240:Carbohydrate Sequence; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D012794:Sialic Acids; D013292:Streptococcus agalactiae / Q000737:chemistry* / Q000276:immunology*
chemical_list: D000942:Antigens, Bacterial; D012794:Sialic Acids