title: Construction, expression and characterisation of a single chain anti-tumour antibody (scFv)-IL-2 fusion protein.
abstract: We describe the production and preliminary characterisation of a fusion protein between interleukin-2 and a single-chain Fv version of the H17E2 anti-placental alkaline phosphatase (PLAP) antibody. This molecule could be used to target interleukin-2 to PLAP-expressing tumours.
authors.affiliation: Department of Immunology, Royal Postgraduate Medical School, Hammersmith Hospital, London, UK.
authors.forename: E
authors.initials: E
authors.lastname: Boleti
authors.forename: M P
authors.initials: MP
authors.lastname: Deonarain
authors.forename: R A
authors.initials: RA
authors.lastname: Spooner
authors.forename: A J
authors.initials: AJ
authors.lastname: Smith
authors.forename: A A
authors.initials: AA
authors.lastname: Epenetos
authors.forename: A J
authors.initials: AJ
authors.lastname: George
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism*; D000912:Antibodies, Neoplasm / Q000378:metabolism*; D001666:Binding Sites, Antibody; D002455:Cell Division; D002460:Cell Line; D003001:Cloning, Molecular; D004797:Enzyme-Linked Immunosorbent Assay; D007376:Interleukin-2 / Q000378:metabolism*; D007527:Isoenzymes / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism*
chemical_list: D000911:Antibodies, Monoclonal; D000912:Antibodies, Neoplasm; C106201:H17E2 monoclonal antibody; D007376:Interleukin-2; D007527:Isoenzymes; D011993:Recombinant Fusion Proteins; C055791:germ-cell AP isoenzyme; D000469:Alkaline Phosphatase