title: Selective photosensitizer distribution in vulvar condyloma acuminatum after topical application of 5-aminolevulinic acid.
abstract: OBJECTIVE
  Our purpose was to determine the feasibility of selective photosensitization of vulvar condylomas by use of tropical application of 5-aminolevulinic acid.
  
  
  METHODS
  In vivo fluorescence was assessed and biopsy specimens of condylomas were taken for fluorescence microscopy in 24 patients at different times after application of 2.5% 5-aminolevulinic acid ointment or 20% 5-aminolevulinic acid cream.
  
  
  RESULTS
  Both in vivo fluorescence imaging and fluorescence microscopy showed selective fluorescence of condylomas of the labia minora and vestibule only within short time intervals, because fluorescence of poorly keratinized normal epithelium was induced by both 5-aminolevulinic acid formulations. In non-hair-bearing skin, lesional fluorescence remained highly selective. Fluorescence microscopy showed that 90 minutes after drug application peak selectivity in epithelial lesional fluorescence was significantly higher with 2.5% 5-aminolevulinic acid ointment (4.5 +/- 0.9) than it was with 20% cream (2.1 +/- 0.2).
  
  
  CONCLUSIONS
  Selective fluorescence of vulvar condyloma acuminatum can be induced by nonselective topical 5-aminolevulinic acid application. Studies evaluating selective photodynamic destruction of condylomas are justified.
authors.affiliation: Beckman Laser Institute and Medical Clinic, University of California, Irvine 92715, USA.
authors.forename: M K
authors.initials: MK
authors.lastname: Fehr
authors.forename: C F
authors.initials: CF
authors.lastname: Chapman
authors.forename: T
authors.initials: T
authors.lastname: Krasieva
authors.forename: B J
authors.initials: BJ
authors.lastname: Tromberg
authors.forename: J L
authors.initials: JL
authors.lastname: McCullough
authors.forename: M W
authors.initials: MW
authors.lastname: Berns
authors.forename: Y
authors.initials: Y
authors.lastname: Tadir
mesh_terms: D000287:Administration, Topical; D000328:Adult; D000622:Aminolevulinic Acid / Q000008:administration & dosage / Q000493:pharmacokinetics* / Q000627:therapeutic use; D003218:Condylomata Acuminata / Q000188:drug therapy* / Q000378:metabolism; D005240:Feasibility Studies; D005260:Female; D006801:Humans; D008856:Microscopy, Fluorescence; D009824:Ointments; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D014018:Tissue Distribution; D014845:Vulvar Diseases / Q000188:drug therapy* / Q000378:metabolism
chemical_list: D009824:Ointments; D017319:Photosensitizing Agents; D000622:Aminolevulinic Acid