title: Exposure to environmental tobacco smoke results in an increased production of (+)-anti-benzo[a]pyrene-7,8-dihydrodiol-9,10-epoxide in juvenile ferret lung homogenates.
abstract: Six-week-old ferrets were exposed head-only to clean air or environmental tobacco smoke (ETS) at an average particulate concentration of 38 +/- 13 mg/m3 for 2 h/d, 5 d/wk for up to 15 wk. Twenty four hours after last exposure, the ferrets were sacrificed and the metabolism of benzo[a]pyrene and (-)-7R-trans-benzo[a]pyrene-7,8-dihydrodiol was studied in lung homogenates. The results show that after ETS exposure total metabolism of benzo[a]pyrene, measured by the accumulation of hexane nonextractable radioactivity, was increased by 35% in the males and 66% in the females (p < .05), respectively, of that observed with air-exposed controls. With (-)-7R-trans-benzo[a]pyrene-7,8-dihydrodiol as substrate, the formation of both benzo[a]pyrene-r-7,t-8,9,c-10-tetrahydrotetraol and (+)-anti-benzo[a]-pyrene-7,8-dihydrodiol-9,10-epoxide-derived tetraols by lung homogenates of ETS-exposed male and female ferrets was significantly increased compared to the air-exposed controls (p < .01). DNA-bound radioactivity was significantly increased in both the males (p < .01) and females (p < .05) compared to the air-exposed ferrets.
authors.affiliation: Department of Pathology, College of Medicine, University of California at Irvine 92717, USA.
authors.forename: R K
authors.initials: RK
authors.lastname: Sindhu
authors.forename: R E
authors.initials: RE
authors.lastname: Rasmussen
authors.forename: Y
authors.initials: Y
authors.lastname: Kikkawa
mesh_terms: D015123:7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide / Q000378:metabolism*; D000818:Animals; D001564:Benzo(a)pyrene / Q000378:metabolism*; D002273:Carcinogens / Q000378:metabolism*; D004247:DNA / Q000378:metabolism; D004781:Environmental Exposure; D005260:Female; D005289:Ferrets; D008168:Lung / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D011247:Pregnancy; D012727:Sex Characteristics; D013237:Stereoisomerism; D014028:Tobacco Smoke Pollution / Q000009:adverse effects*
chemical_list: D002273:Carcinogens; D014028:Tobacco Smoke Pollution; D001564:Benzo(a)pyrene; D015123:7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide; D004247:DNA