title: The aerial mycelium-defective phenotype of Streptomyces griseus resulting from A-factor deficiency is suppressed by a Ser/Thr kinase of S. coelicolor A3(2).
abstract: A-factor (2-isocapryloyl-3R-hydroxymethyl-gamma-butyrolactone) is essential for aerial mycelium formation and streptomycin (Sm) production in Streptomyces griseus. A protein Ser/Thr kinase (AfsK), the product of the Streptomyces coelicolor A3(2) afsK gene, controlling secondary metabolism in this strain, reversed the aerial mycelium-negative phenotype of an A-factor-deficient mutant strain, S. griseus HH1, and induced sporulation without affecting A-factor productivity or Sm production. A mutant AfsK protein lacking kinase activity failed to induce aerial mycelium formation which indicates the importance of the kinase activity for suppression in S. griseus. These data suggest that a Ser/Thr kinase functionally similar to S. coelicolor A3(2) AfsK plays a regulatory role in aerial mycelium formation in S. griseus, either as a member in the A-factor regulatory network or independently of this network.
authors.affiliation: Department of Biotechnology, The University of Tokyo, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Ueda
authors.forename: T
authors.initials: T
authors.lastname: Umeyama
authors.forename: T
authors.initials: T
authors.lastname: Beppu
authors.forename: S
authors.initials: S
authors.lastname: Horinouchi
mesh_terms: D015107:4-Butyrolactone / Q000031:analogs & derivatives / Q000378:metabolism; D002227:Carbazoles / Q000494:pharmacology; D002454:Cell Differentiation; D004791:Enzyme Inhibitors / Q000494:pharmacology; D016153:Genes, Suppressor; D026121:Indole Alkaloids; D011493:Protein Kinase C / Q000037:antagonists & inhibitors; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D015183:Restriction Mapping; D013171:Spores, Bacterial / Q000166:cytology; D013302:Streptomyces / Q000201:enzymology*; D013305:Streptomyces griseus / Q000166:cytology / Q000201:enzymology*
chemical_list: D002227:Carbazoles; D004791:Enzyme Inhibitors; D026121:Indole Alkaloids; C025035:A-factor (Streptomyces); C049985:staurosporine aglycone; D017346:Protein Serine-Threonine Kinases; D011493:Protein Kinase C; D015107:4-Butyrolactone