title: NP-06: a novel anti-human immunodeficiency virus polypeptide produced by a Streptomyces species.
abstract: From an extract of a Streptomyces culture, we identified and purified a novel compound, NP-06, which is active against human immunodeficiency virus (HIV) in vitro. Analyses indicate that NP-06 is a hydrophobic 21-mer oligopeptide, N terminally cyclized through the side chain of Asp-9, containing two intramolecular cystine linkages with a molecular weight of 2,163.4. The 50% inhibitory concentrations were 2.8 and 1.3 microM when NP-06 was tested for in vitro anti-HIV-1 activity in ATH8 cells and phytohemagglutinin-activated peripheral blood mononuclear cells, respectively, NP-06 appears to block the early stage of HIV-1 infection, most likely at the stage of virus-cell fusion.
authors.affiliation: Medicine Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA.
authors.forename: S
authors.initials: S
authors.lastname: Chokekijchai
authors.forename: E
authors.initials: E
authors.lastname: Kojima
authors.forename: S
authors.initials: S
authors.lastname: Anderson
authors.forename: M
authors.initials: M
authors.lastname: Nomizu
authors.forename: M
authors.initials: M
authors.lastname: Tanaka
authors.forename: M
authors.initials: M
authors.lastname: Machida
authors.forename: T
authors.initials: T
authors.lastname: Date
authors.forename: K
authors.initials: K
authors.lastname: Toyota
authors.forename: S
authors.initials: S
authors.lastname: Ishida
authors.forename: K
authors.initials: K
authors.lastname: Watanabe
mesh_terms: D000595:Amino Acid Sequence; D000998:Antiviral Agents / Q000737:chemistry / Q000494:pharmacology*; D006678:HIV / Q000187:drug effects*; D006801:Humans; D008969:Molecular Sequence Data; D010456:Peptides, Cyclic / Q000737:chemistry / Q000494:pharmacology*; D013302:Streptomyces / Q000378:metabolism*
chemical_list: D000998:Antiviral Agents; C097450:NP-06 cyclic peptide; D010456:Peptides, Cyclic; C085040:RP 71955