title: Persistent activation of CDK4 during neuronal differentiation of rat pheochromocytoma PC12 cells.
abstract: It has been shown that suppression of cyclin-dependent kinase 4 (CDK4) expression or its kinase activity is a critical event for the differentiation of hematopoietic and myoblastic cells. To investigate the role of CDK4 during NGF-mediated neuronal differentiation of PC12 cells, we examined the changes in the expression and kinase activity of CDK4. Unexpectedly, both the expression of CDK4 and its kinase activity remained high throughout 10 days of exposure to NGF. Furthermore, constitutive overexpression of the human CDK4 did not inhibit the differentiation. These results suggest that suppression of CDK4 is not required for the NGF-induced neuronal differentiation of PC12 cells.
authors.affiliation: Department of Oncogene Research, Research Institute for Microbial Diseases, Osaka University, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Dobashi
authors.forename: T
authors.initials: T
authors.lastname: Kudoh
authors.forename: K
authors.initials: K
authors.lastname: Toyoshima
authors.forename: T
authors.initials: T
authors.lastname: Akiyama
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D051358:Cyclin-Dependent Kinase 4; D018844:Cyclin-Dependent Kinases / Q000378:metabolism*; D018076:DNA, Complementary; D004789:Enzyme Activation; D006801:Humans; D008969:Molecular Sequence Data; D009414:Nerve Growth Factors / Q000494:pharmacology; D009474:Neurons / Q000166:cytology*; D016716:PC12 Cells; D011518:Proto-Oncogene Proteins; D051381:Rats
chemical_list: D018076:DNA, Complementary; D009414:Nerve Growth Factors; D011518:Proto-Oncogene Proteins; C495900:CDK4 protein, human; C495903:Cdk4 protein, rat; D051358:Cyclin-Dependent Kinase 4; D018844:Cyclin-Dependent Kinases