title: Nucleotide sequence and characterization of erythromycin resistance determinant that encodes macrolide 2'-phosphotransferase I in Escherichia coli.
abstract: The DNA fragment (3.3 kb) containing the erythromycin resistance determinant was cloned from Escherichia coli Tf481A and sequenced. Deletion and complementation analyses indicated that the expression of high-level resistance to erythromycin requires two genes, mphA and mrx, which encode macrolide 2'-phosphotransferase I and an unidentified hydrophobic protein, respectively.
authors.affiliation: Department of Microbiology, School of Pharmacy, Tokyo University of Pharmacy and Life Science, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Noguchi
authors.forename: A
authors.initials: A
authors.lastname: Emura
authors.forename: H
authors.initials: H
authors.lastname: Matsuyama
authors.forename: K
authors.initials: K
authors.lastname: O'Hara
authors.forename: M
authors.initials: M
authors.lastname: Sasatsu
authors.forename: M
authors.initials: M
authors.lastname: Kono
mesh_terms: D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000378:metabolism* / Q000494:pharmacology*; D001483:Base Sequence; D004352:Drug Resistance, Microbial / Q000235:genetics*; D004917:Erythromycin / Q000494:pharmacology*; D004926:Escherichia coli / Q000201:enzymology*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010770:Phosphotransferases / Q000235:genetics*; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D004917:Erythromycin; D010770:Phosphotransferases; D017853:Phosphotransferases (Alcohol Group Acceptor); C061369:macrolide 2'-kinase