Restriction enzyme buffers and 5-azacytidine induce loss of protein and banding in plant metaphase chromosomes.
The incubation of root meristem of Vicia faba L. with 5-azacytidine for 72 h (5 cell cycles) resulted in shorter DNA fragments after digestion with a restriction enzyme Sau3A. Under the same condition in situ digestion of metaphase chromosomes with Sau3A revealed a stronger banding (C-banding-like) pattern in 5-azacytidine-treated chromosomes than in the control. This difference could hardly be attributed to the euchromatin demethylation as 5-azacytidine caused a loss of chromosomal proteins; similar effect was found as a result of incubation with some restriction endonuclease buffers which did not diminish the labeling with 3H-thymidine. After 5-azacytidine administration the entrance of cells into mitosis was delayed, the metaphase chromosomes were undercondensed and the pattern of DNA replication remained unchanged.
Olszewska
M J
MJ
Department of Plant Cytology and Cytochemistry, University of Lódź, Poland.
Gernand
D
D
Luchniak
P
P
Sakowicz
T
T
D001374:Azacitidine / Q000494:pharmacology*; D002021:Buffers; D002871:Chromosome Banding; D002875:Chromosomes / Q000187:drug effects*; D004262:DNA Restriction Enzymes / Q000494:pharmacology*; D018744:DNA, Plant / Q000032:analysis / Q000187:drug effects / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific; D007887:Fabaceae; D007621:Karyotyping; D008677:Metaphase / Q000235:genetics; D008745:Methylation; D010940:Plant Proteins / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects / Q000378:metabolism; D010946:Plants, Medicinal
D002021:Buffers; D018744:DNA, Plant; D010940:Plant Proteins; D004262:DNA Restriction Enzymes; D015252:Deoxyribonucleases, Type II Site-Specific; C024405:GATC-specific type II deoxyribonucleases; D001374:Azacitidine