Estrogen regulation of JE/MCP-1 mRNA expression in fibroblasts.
We have recently demonstrated that 17 beta-estradiol (E2) inhibits peritoneal adhesion formation. Because macrophages play a central role in inflammation and wound healing, we chose to investigate whether the E2 could inhibit the expression of JE, the murine monocyte chemoattractant protein-1 (MCP-1). To accomplish this, murine fibroblasts were cultured with physiological concentrations of E2 (3-300 pg/ml) with or without inducers of JE/MCP-1 mRNA expression. Untreated cells failed to express the message, but, following stimulation, a marked increase in JE/MCP-1 mRNA expression was observed. At 10-30 pg/ml, E2 had no effect on JE/MCP-1 mRNA expression in stimulated fibroblasts. In contrast, lower and higher doses of E2 inhibited the expression of JE/MCP-1 mRNA in stimulated fibroblasts. Treatment with tamoxifen reversed the E2-inhibition of expression of the message. These data demonstrate that JE/MCP-1 mRNA expression is controlled, in part, by estrogen and suggest that macrophage recruitment may be affected by circulating levels of E2.
Kovacs
E J
EJ
Department of Cell Biology, Loyola University, Maywood, IL, USA.
Faunce
D E
DE
Ramer-Quinn
D S
DS
Mott
F J
FJ
Dy
P W
PW
Frazier-Jessen
M R
MR
D016475:3T3 Cells / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D000818:Animals; D002478:Cells, Cultured; D018932:Chemokine CCL2 / Q000096:biosynthesis* / Q000235:genetics; D003907:Dexamethasone / Q000494:pharmacology; D004958:Estradiol / Q000494:pharmacology*; D004965:Estrogen Antagonists / Q000494:pharmacology; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism* / Q000502:physiology; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D051379:Mice; D010982:Platelet-Derived Growth Factor / Q000037:antagonists & inhibitors / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D012680:Sensitivity and Specificity; D013629:Tamoxifen / Q000494:pharmacology
D018932:Chemokine CCL2; D004965:Estrogen Antagonists; D010982:Platelet-Derived Growth Factor; D012333:RNA, Messenger; D013629:Tamoxifen; D004958:Estradiol; D003907:Dexamethasone