title: Brush border enzymes in evaluation of acute small bowel rejection.
abstract: The change in activity of three disaccharidase enzymes (maltase, sucrase and lactase) was determined according to the method of Dahlqvist during acute rejection in non-immunosuppressed piglet small bowel grafts. In addition, two brush border enzymes, lactase and aminopeptidase, were stained with monoclonal antibodies. Diminishing disaccharidase activity was an early event during rejection. Diminution began 2 days before distinct morphological changes were seen in the mucosal biopsies. Evaluation of disaccharidase activity can thus be used as a confirmatory method in detecting rejection. Reduction in immunohistological staining of lactase and aminopeptidase with monoclonal antibodies and changes in mucosal morphology were observed to progress simultaneously.
authors.affiliation: Second Department of Surgery, Helsinki University Central Hospital, Finland.
authors.forename: P
authors.initials: P
authors.lastname: Kuusanmäki
authors.forename: J
authors.initials: J
authors.lastname: Lauronen
authors.forename: T
authors.initials: T
authors.lastname: Paavonen
authors.forename: M
authors.initials: M
authors.lastname: Pakarinen
authors.forename: E
authors.initials: E
authors.lastname: Savilahti
authors.forename: P
authors.initials: P
authors.lastname: Häyry
authors.forename: J
authors.initials: J
authors.lastname: Halttunen
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D001706:Biopsy; D004186:Disaccharidases / Q000378:metabolism; D005260:Female; D006084:Graft Rejection / Q000201:enzymology*; D007150:Immunohistochemistry; D007421:Intestine, Small / Q000033:anatomy & histology / Q000473:pathology / Q000637:transplantation*; D043322:Lactase; D008871:Microvilli / Q000201:enzymology*; D013194:Staining and Labeling; D013393:Sucrase / Q000378:metabolism; D013552:Swine; D000520:alpha-Glucosidases / Q000378:metabolism; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D004186:Disaccharidases; D043322:Lactase; D000520:alpha-Glucosidases; D001616:beta-Galactosidase; D013393:Sucrase