Detection of the gene for toxic shock syndrome toxin 1 in Staphylococcus aureus by enzyme-labelled oligonucleotide probes.
A colony hybridisation method with enzyme-labelled oligonucleotide probes was developed to detect the gene for toxic shock syndrome toxin 1 (tst). For rapid identification, bacterial colonies were transferred from agar plates directly on to nylon membranes. These procedures took only 3 h. Results obtained by this test correlated well with those obtained by the reverse passive latex agglutination test. Thus, this method is convenient and reliable for the detection of tst in staphylococci, which could be useful for both research and clinical purposes. This method demonstrated that tst was more prevalent in methicillin-resistant Staphylococcus aureus (56%) than in methicillin-susceptible S. aureus (4%).
Shimaoka
M
M
Department of Bacterial Infections, Research Institute for Microbial Diseases, Osaka University, Japan.
Yoh
M
M
Takarada
Y
Y
Yamamoto
K
K
Honda
T
T
D001427:Bacterial Toxins / Q000235:genetics*; D001483:Base Sequence; D004768:Enterotoxins / Q000235:genetics*; D005798:Genes, Bacterial; D007841:Latex Fixation Tests; D008712:Methicillin / Q000494:pharmacology; D016106:Methicillin Resistance; D015336:Molecular Probe Techniques; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes; D010406:Penicillins / Q000494:pharmacology; D013211:Staphylococcus aureus / Q000235:genetics*; D018089:Superantigens
D001427:Bacterial Toxins; D004768:Enterotoxins; D015345:Oligonucleotide Probes; D010406:Penicillins; D018089:Superantigens; C030107:enterotoxin F, Staphylococcal; D008712:Methicillin