title: Identification of multiple plasmids released from recombinant genomes of Hansenula polymorpha by transformation of Escherichia coli.
abstract: Total DNAs isolated from two Hansenula polymorpha (Pichia angusta) strains having chromosomal single or tandem multiple integrations of a pUC18-derived expression plasmid produced Escherichia coli transformants which contained plasmids of different size and/or organization than that of the expression plasmid. Evidence that plasmid-like structures are formed in H. polymorpha and that their formation is stimulated by DNA damage is presented in this study.
authors.affiliation: Fachbereich Biologie, Universität Oldenburg, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Graupner
authors.forename: W
authors.initials: W
authors.lastname: Wackernagel
mesh_terms: D004926:Escherichia coli / Q000235:genetics*; D010843:Pichia / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D014169:Transformation, Bacterial