Leishmania chagasi: a gene encoding a protein kinase with a catalytic domain structurally related to MAP kinase kinase.
The gene for a serine/threonine protein kinase was isolated from Leishmania chagasi. The deduced amino acid sequence encoded by this Leishmania protein kinase (LPK-1) gene possesses all of the 11 conserved subdomains found in most other serine/threonine protein kinase catalytic domains. Sequence alignment with other known protein kinases demonstrates that the 42-kDa LPK-1 is a member of the mitogen-activated protein kinase kinase family involved in the signal-transduction pathways in yeast and mammalian cells. The single copy gene for LPK-1 specifies a 3.1-kb mRNA that is expressed in both logarithmic and stationary phase promastigotes with a twofold higher steady-state level in the infective stationary phase promastigotes.
Li
S
S
Department of Biochemistry, University of Iowa, Iowa City 52242, USA.
Wilson
M E
ME
Donelson
J E
JE
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D017124:Conserved Sequence; D017931:DNA Primers / Q000737:chemistry; D016054:DNA, Protozoan / Q000737:chemistry; D018503:Genome, Protozoan; D006801:Humans; D018314:Leishmania infantum / Q000201:enzymology / Q000235:genetics*; D020929:Mitogen-Activated Protein Kinase Kinases; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D016133:Polymerase Chain Reaction; D011494:Protein Kinases / Q000737:chemistry / Q000235:genetics*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction
D017931:DNA Primers; D016054:DNA, Protozoan; D011494:Protein Kinases; D020929:Mitogen-Activated Protein Kinase Kinases