title: Isolation of Rhodobacter capsulatus transketolase: cloning and sequencing of its structural tktA gene.
abstract: Rhodobacter capsulatus transketolase (Tkt) protein has been isolated from strain B10 by heparin affinity chromatography. Oligodeoxyribonucleotides (oligo) constructed as based on the amino-acid sequences were used for polymerase chain reaction (PCR) amplification on total genomic DNA. Southern hybridization with the PCR product as a probe allowed the isolation of a 5-kb PstI DNA fragment containing the structural Tkt-encoding gene (tktA) which was cloned and sequenced. The deduced tktA product of 671 aa (72815 Da) shares 59% identity with Rhodobacter sphaeroides Tkt.
authors.affiliation: CEA Grenoble, Biochimie Microbienne, Département de Biologie Moléculaire et Structurale, France.
authors.forename: R
authors.initials: R
authors.lastname: de Sury d'Aspremont
authors.forename: B
authors.initials: B
authors.lastname: Toussaint
authors.forename: P M
authors.initials: PM
authors.lastname: Vignais
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000302:isolation & purification; D016354:Rhodobacter capsulatus / Q000235:genetics*; D014174:Transketolase / Q000235:genetics*
chemical_list: D011994:Recombinant Proteins; D014174:Transketolase