The rat FGF-5 mRNA variant generated by alternative splicing encodes a novel truncated form of FGF-5.
We isolated rat FGF-5 cDNA and its variant from the embryo. The FGF-5 cDNA encodes a protein of 266 amino acid residues. The variant cDNA encodes a protein of 121 amino acid residues, the N-terminal 117-amino acid sequence of which is identical to that of FGF-5. Thus the variant cDNA encodes a novel truncated form of FGF-5. The rat FGF-5 gene consists of three exons (exons 1-3). The variant cDNA lacks the nucleotide sequence of exon 2, indicating that the variant mRNA is generated by alternative splicing. The variant mRNA as well as the FGF-5 mRNA was detected in the embryo and adult brain. This is the first description of the mRNA for a truncated form of FGF within the FGF family.
Hattori
Y
Y
Department of Genetic Biochemistry, Kyoto University Faculty of Pharmaceutical Sciences, Japan.
Yamasaki
M
M
Itoh
N
N
D000367:Age Factors; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000254:growth & development / Q000378:metabolism; D018076:DNA, Complementary / Q000235:genetics; D005326:Fetal Proteins / Q000235:genetics; D051521:Fibroblast Growth Factor 5; D005346:Fibroblast Growth Factors / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D014644:Genetic Variation; D051379:Mice; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000096:biosynthesis / Q000235:genetics; D009928:Organ Specificity; D012326:RNA Splicing; D012333:RNA, Messenger / Q000235:genetics*; D051381:Rats; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
D018076:DNA, Complementary; D005326:Fetal Proteins; C496450:Fgf5 protein, mouse; C496451:Fgf5 protein, rat; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D051521:Fibroblast Growth Factor 5; D005346:Fibroblast Growth Factors