Production of a novel monoclonal antibody, JT-95, which can detect antigen of thyroid carcinoma.
Monoclonal antibody (MAb) JT-95 was produced by immunization of mice with membrane fractions of a human thyroid carcinoma. Immuno-histochemical staining has demonstrated that the antigen recognized by JT-95 is strongly expressed in 95 (95%) of 100 cases of papillary carcinomas and in 3 (75%) of 4 cases of follicular carcinomas. In benign diseases of the thyroid gland, MAb JT-95 reacted with 0 (0%) of 39 adenomas, 1 (4%) of 21 adenomatous goiters, 0 (0%) of 8 hyperthyroidism specimens, and 3 (38%) of 8 chronic thyroiditis specimens. The antigen detected by MAb JT-95 has an apparent Mr 250,000 in thyroid carcinomas. Moreover, circulating antigen in thyroid carcinoma patients was detected by MAb JT-95 in an ELISA and in Western blotting. The circulating antigen has a Mr 105,000. MAb JT-95 conjugated with (131) I was administrated to nude mice bearing a human thyroid carcinoma. JT-95 131I accumulation at the transplanted tumor was visualized by autoradiography with 2.68-14.75-fold higher levels detected at the xenograft compared to that for normal organs. Based on these data, MAb JT-95 may be useful in the diagnosis detection and therapy of thyroid carcinoma.
Takeyama
H
H
First Department of Surgury, Jikei University School of Medicine, Tokyo, Japan.
Hosoya
T
T
Sakurai
K
K
Mori
Y
Y
Watanabe
M
M
Kisaki
H
H
Ohno
T
T
D018263:Adenocarcinoma, Follicular / Q000473:pathology; D000236:Adenoma / Q000473:pathology; D000818:Animals; D000911:Antibodies, Monoclonal / Q000493:pharmacokinetics; D000951:Antigens, Neoplasm / Q000032:analysis*; D000954:Antigens, Surface / Q000032:analysis*; D001345:Autoradiography; D002291:Carcinoma, Papillary / Q000473:pathology; D003001:Cloning, Molecular; D004797:Enzyme-Linked Immunosorbent Assay; D006042:Goiter / Q000473:pathology; D006801:Humans; D006980:Hyperthyroidism / Q000473:pathology; D007150:Immunohistochemistry; D007457:Iodine Radioisotopes; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology; D008819:Mice, Nude; D012016:Reference Values; D012680:Sensitivity and Specificity; D013959:Thyroid Diseases / Q000473:pathology*; D013964:Thyroid Neoplasms / Q000473:pathology*; D014018:Tissue Distribution; D014183:Transplantation, Heterologous
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D007457:Iodine Radioisotopes