In vivo evaluation of a collagen corneal allograft derived from rabbit dermis.
BACKGROUND
This study evaluates epithelialization, clarity, intraocular inflammation, and fibroblast ingrowth of a collagen corneal allograft derived from rabbit dermis.


METHODS
Dermal collagen fibers were dispersed intact and chemically modified to make them soluble. The allografts consisted of a fibrous, opaque peripheral zone and a central clear area. Twelve New Zealand white rabbits underwent penetrating keratoplasty with implantation of an allograft. The grafts were evaluated daily for clarify, anterior segment inflammation, and extent of reepithelialization with a slit-lamp microscope.


RESULTS
Epithelialization of the fibrous peripheral zone of the graft ranged from 0% to 90%. The central clear area did not epithelialize in any of the animals. Fibroblasts migrated into the peripheral zone in all eyes. Complications included ulceration of the central clear area in two eyes. There was no ulceration or leakage at the graft-host interface and no synechia, fibrous, or inflammatory retrocorneal membranes in any of the eyes.


CONCLUSIONS
Our study is the first to describe the method of modifying dermal type I collagen into a clear corneal allograft. Survival of the corneal collagen allograft beyond 1 month may be limited by several factors including lack of epithelialization of the central clear area.
Kornmehl
E W
EW
Cornea and Anterior Sergment Service, New England Eye Center, Tufts University School of Medicine, Boston, USA.
Bredvik
B K
BK
Kelman
C D
CD
Raizman
M B
MB
DeVore
D P
DP
D000818:Animals; D000869:Anterior Eye Segment / Q000473:pathology; D003094:Collagen / Q000302:isolation & purification; D003315:Cornea / Q000473:pathology / Q000503:physiopathology / Q000601:surgery*; D016039:Corneal Transplantation; D004848:Epithelium / Q000503:physiopathology; D007249:Inflammation; D008854:Microscopy, Electron; D011817:Rabbits; D012038:Regeneration; D012867:Skin / Q000737:chemistry*; D014184:Transplantation, Homologous
D003094:Collagen