Fluorescence in situ hybridization on human metaphase chromosomes detected by near-field scanning optical microscopy.
Fluorescence in situ hybridization on human metaphase chromosomes is detected by near-field scanning optical microscopy. This combination of cytochemical and scanning probe techniques enables the localization and identification of several fluorescently labelled genomic DNA fragments on a single chromosome with an unprecedented resolution. Three nucleic acid probes are used: pUC1.77, p1-79 and the plasmid probe alpha-spectrin. The hybridization signals are very well resolved in the near-field fluorescence images, while the exact location of the probes can be correlated accurately with the chromosome topography as afforded by the shear force image.
Moers
M H
MH
Department of Applied Physics, University of Twente, AE Enschede, The Netherlands.
Kalle
W H
WH
Ruiter
A G
AG
Wiegant
J C
JC
Raap
A K
AK
Greve
J
J
de Grooth
B G
BG
van Hulst
N F
NF
D001710:Biotin; D002503:Centromere / Q000648:ultrastructure; D002875:Chromosomes / Q000737:chemistry* / Q000648:ultrastructure; D004247:DNA / Q000032:analysis* / Q000235:genetics; D015342:DNA Probes; D004076:Digoxigenin; D016650:Fluorescein-5-isothiocyanate; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008214:Lymphocytes; D008297:Male; D008677:Metaphase; D008856:Microscopy, Fluorescence; D013049:Spectrin / Q000235:genetics; D016615:Telomere / Q000648:ultrastructure
D015342:DNA Probes; D013049:Spectrin; D001710:Biotin; D004247:DNA; D016650:Fluorescein-5-isothiocyanate; D004076:Digoxigenin