Pumpkin hydroxypyruvate reductases with and without a putative C-terminal signal for targeting to microbodies may be produced by alternative splicing.
Two full-length cDNAs encoding hydroxypyruvate reductase were isolated from a cDNA library constructed with poly(A)+ RNA from pumpkin green cotyledons. One of the cDNAs, designated HPR1, encodes a polypeptide of 386 amino acids, while the other cDNA, HPR2 encodes a polypeptide of 381 amino acids. Although the nucleotide and deduced amino acid sequences of these cDNAs are almost identical, the deduced HPR1 protein contains Ser-Lys-Leu at its carboxy-terminal end, which is known as a microbody-targeting signal, while the deduced HPR2 protein does not. Analysis of genomic DNA strongly suggests that HPR1 and HPR2 are produced by alternative splicing.
Hayashi
M
M
Department of Cell Biology, National Institute for Basic Biology, Okazaki, Japan.
Tsugeki
R
R
Kondo
M
M
Mori
H
H
Nishimura
M
M
D000429:Alcohol Oxidoreductases / Q000235:genetics*; D017398:Alternative Splicing; D000595:Amino Acid Sequence; D001483:Base Sequence; D001692:Biological Transport; D002451:Cell Compartmentation; D018548:Cotyledon / Q000201:enzymology; D018076:DNA, Complementary / Q000235:genetics; D015723:Gene Library; D050537:Hydroxypyruvate Reductase; D007527:Isoenzymes / Q000235:genetics*; D008830:Microbodies / Q000201:enzymology*; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014675:Vegetables / Q000201:enzymology / Q000235:genetics*
D018076:DNA, Complementary; D007527:Isoenzymes; D010940:Plant Proteins; D000429:Alcohol Oxidoreductases; D050537:Hydroxypyruvate Reductase