Phospholipase D signaling is essential for meiosis.
Phospholipid metabolism plays an important role in cellular regulation by generating second messengers for signal transduction. Many stimuli activate a phospholipase D, which catalyzes the hydrolysis of phosphatidylcholine, producing phosphatidic acid and choline. Here we report that the yeast SP014 gene, which is essential for meiosis [Honigberg, S. M., Conicella, C. & Esposito, R. E. (1992) Genetics 130, 703-716], encodes a phospholipase D. SP014 RNA and protein activity are induced during late meiotic prophase, and the enzyme has properties similar to mammalian phosphatidylinositol 4,5-bisphosphate-regulated phospholipase D. Characterization of an unusual allele of SP014 defines regions of the protein important for enzyme catalysis and regulation. These results implicate phospholipase D signaling in regulating cellular differentiation.
Rose
K
K
Department of Pharmacology, State University of New York, Stony Brook 11794-8651, USA.
Rudge
S A
SA
Frohman
M A
MA
Morris
A J
AJ
Engebrecht
J
J
D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D005800:Genes, Fungal; D008322:Mammals; D008540:Meiosis / Q000235:genetics / Q000502:physiology*; D008969:Molecular Sequence Data; D010739:Phospholipase D / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction; D014162:Transfection
D011994:Recombinant Proteins; D010739:Phospholipase D