Comparison of latex-specific IgE binding among nonammoniated latex, ammoniated latex, and latex glove allergenic extracts by ELISA and immunoblot inhibition.
BACKGROUND
Nonammoniated latex, ammoniated latex, and latex glove extracts have been used as source materials for the preparation of allergenic extracts for the diagnosis of latex allergy. These materials showed different patterns of protein bands and immunoreactive bands. However, their IgE-reactive repertoires were not compared.


OBJECTIVE
The goals of this study were to compare the IgE reactivity and to define the common IgE-reactive epitopes among three latex allergenic extracts.


METHODS
Two serum pools were obtained from adults and children with latex allergy to evaluate the IgE reactivity among three latex extracts. IgE reactivity and IgE-reactive proteins were compared by inhibition ELISA and inhibition immunoblot methods, respectively.


RESULTS
In this study inhibition curves were similar for nonammoniated latex and ammoniated latex but were different when the latex glove extracts were used. Several protein bands of ammoniated latex and latex glove extracts could not be inhibited by the nonammoniated latex. The ammoniated latex and latex glove extracts were able to remove all the latex-specific IgE from the serum.


CONCLUSIONS
The IgE-reactive proteins differ among different latex extracts. Ammoniated latex and latex glove extracts contain more complete immunoreactive repertoires for detecting IgE antibodies. Our study provides useful information for selecting the latex extract.
Akasawa
A
A
Laboratory of Immunobiochemistry, Food and Drug Administration, Rockville, MD 20852-1441,USA.
Hsieh
L S
LS
Lin
Y
Y
D000328:Adult; D000641:Ammonia / Q000378:metabolism; D001666:Binding Sites, Antibody; D001667:Binding, Competitive; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D015151:Immunoblotting; D007073:Immunoglobulin E / Q000378:metabolism*; D007840:Latex / Q000378:metabolism*; D012408:Rubber / Q000378:metabolism*
D007840:Latex; D007073:Immunoglobulin E; D000641:Ammonia; D012408:Rubber