The use of non-uniform deuterium labelling ['NMR-window'] to study the NMR structure of a 21mer RNA hairpin.
The first synthesis of a non-uniformly deuterium labelled 21mer RNA is reported using our 'NMR-window' concept, showing its unique application in the unambiguous NMR assignment of the non-exchangeable aromatic and sugar protons.
Földesi
A
A
Department of Bioorganic Chemistry, Biomedical Center, Uppsala University, Sweden.
Yamakage
S I
SI
Nilsson
F P
FP
Maltseva
T V
TV
Chattopadhyaya
J
J
D003903:Deuterium; D006860:Hydrogen Bonding; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D009690:Nucleic Acid Conformation; D009843:Oligoribonucleotides / Q000737:chemistry*
D009843:Oligoribonucleotides; D003903:Deuterium