High cell density cultivation and high recombinant protein production of Escherichia coli strain expressing uricase.
Uricase from Cellulomonas flavigena SK-4 is an industrially useful enzyme for commercial formulations of hair coloring. The uricase production by recombinant Escherichia coli strain with a high cell density cultivation technique was described. Of three kinds of media, synthetic media with the feeding of a high concentration of glucose solution were suitable for high cell density cultivation. As for feeding, both biomass concentration and uricase productivity were increased by about two (61.2 g dry cell weight (DCW)/liter) and three times (1037 U/ml broth), respectively, in 24 h by continuous supply. In the case of feeding by a DO-stat method, however, cell concentration was comparable to continuous glucose supply but uricase activity was reduced. By supplying pure oxygen to compensate for oxygen limitation during cultivation, the highest values of 77.4g DCW/liter and 1113 U/ml broth of the uricase activity were achieved with the total cultivation time of 15 h.
Nakagawa
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S
Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., Japan.
Oda
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H
Anazawa
H
H
D000192:Actinomycetales / Q000201:enzymology; D001431:Bacteriological Techniques; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D005656:Fungal Proteins / Q000096:biosynthesis* / Q000235:genetics; D005947:Glucose / Q000494:pharmacology; D010100:Oxygen / Q000494:pharmacology; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D014503:Urate Oxidase / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism
D005656:Fungal Proteins; D011994:Recombinant Proteins; D014503:Urate Oxidase; D005947:Glucose; D010100:Oxygen