title: Differential expression of chemokines in patients with localized and diffuse cutaneous American leishmaniasis.
abstract: The abundance of macrophages in localized cutaneous leishmaniasis (LCL) and diffuse cutaneous leishmaniasis (DCL) lesions and differences in the composition of T cell subsets indicate involvement of cell-specific chemotaxis processes. The expression of macrophage chemoattractant protein (MCP)-1, macrophage inflammatory protein (MIP)-1 alpha and -1 beta, RANTES (regulated on activation, normal T cell expressed and secreted), I-309, and interleukin-8 were investigated in lesions of patients with LCL or DCL. In LCL, high levels of MCP-1 and moderate levels of MIP-1 alpha were detected. In DCL, MCP-1 expression was significantly lower and MIP-1 alpha expression was predominant. All other chemokines investigated were minimally expressed or absent. These findings suggest that MCP-1 and MIP-alpha are responsible for the recruitment of macrophages and T cells in cutaneous leishmaniasis. The results show that self-healing LCL is associated with higher levels of MCP-1, which may stimulate macrophage microbicidal mechanisms, and nonhealing DCL is associated with higher levels of MIP-alpha.
authors.affiliation: Infectious Diseases Research Center, University of Würzburg, Germany.
authors.forename: U
authors.initials: U
authors.lastname: Ritter
authors.forename: H
authors.initials: H
authors.lastname: Moll
authors.forename: T
authors.initials: T
authors.lastname: Laskay
authors.forename: E
authors.initials: E
authors.lastname: Bröcker
authors.forename: O
authors.initials: O
authors.lastname: Velazco
authors.forename: I
authors.initials: I
authors.lastname: Becker
authors.forename: R
authors.initials: R
authors.lastname: Gillitzer
mesh_terms: D000818:Animals; D001483:Base Sequence; D054402:Chemokine CCL1; D018932:Chemokine CCL2 / Q000378:metabolism; D054407:Chemokine CCL4; D018946:Chemokine CCL5 / Q000378:metabolism; D018925:Chemokines / Q000378:metabolism*; D019742:Chemokines, CC; D002633:Chemotaxis; D016207:Cytokines / Q000378:metabolism; D017931:DNA Primers / Q000235:genetics; D016054:DNA, Protozoan / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D016209:Interleukin-8 / Q000378:metabolism; D007894:Leishmania mexicana / Q000235:genetics / Q000302:isolation & purification; D016773:Leishmaniasis, Cutaneous / Q000276:immunology* / Q000469:parasitology / Q000473:pathology; D016774:Leishmaniasis, Diffuse Cutaneous / Q000276:immunology* / Q000469:parasitology / Q000473:pathology; D019402:Macrophage Inflammatory Proteins; D008264:Macrophages / Q000276:immunology; D008969:Molecular Sequence Data; D015846:Monokines / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: C065971:CCL1 protein, human; D054402:Chemokine CCL1; D018932:Chemokine CCL2; D054407:Chemokine CCL4; D018946:Chemokine CCL5; D018925:Chemokines; D019742:Chemokines, CC; D016207:Cytokines; D017931:DNA Primers; D016054:DNA, Protozoan; D016209:Interleukin-8; D019402:Macrophage Inflammatory Proteins; D015846:Monokines