title: A Cdc2 dependent checkpoint maintains diploidy in Drosophila.
abstract: DNA replication in G2 does not normally occur due to the checkpoint control. To elucidate its mechanism, the functions of the escargot and Dmcdc2 genes of Drosophila were studied. When escargot function was eliminated, diploid imaginal cells that were arrested in G2 lost Cyclin A, a regulatory subunit of G2/M cdk, and entered an endocycle. escargot genetically interacted with Dmcdc2 which encodes a catalytic subunit of G2/M cdk. The mutant phenotypes of Dmcdc2 itself was similar to those of escargot: many diploid cells in imaginal discs, salivary glands and the central nervous system entered an endocycle and sometimes formed polytene chromosomes. Since mitotically quiescent abdominal histoblasts still required Dmcdc2 to remain diploid, the inhibitory activity of G2/M cdk on DNA replication appeared to be separable from its activity as the mitosis promoting factor. These results suggest that in G2, escargot is required to maintain a high level of G2/M cdk that actively inhibits the entry into S phase.
authors.affiliation: National Institute of Genetics, Mishima, Shizuoka-ken, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Hayashi
mesh_terms: D000818:Animals; D016203:CDC2 Protein Kinase / Q000378:metabolism / Q000502:physiology*; D002453:Cell Cycle / Q000502:physiology*; D018797:Cell Cycle Proteins; D016213:Cyclins / Q000032:analysis; D004261:DNA Replication / Q000502:physiology; D004171:Diploidy; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000166:cytology* / Q000235:genetics; D016195:G2 Phase / Q000502:physiology; D015870:Gene Expression; D017344:Genes, Insect; D018869:Heat-Shock Response; D007814:Larva; D008938:Mitosis / Q000502:physiology; D009154:Mutation; D010749:Phosphoprotein Phosphatases / Q000096:biosynthesis / Q000235:genetics; D017027:Protein Tyrosine Phosphatases; D014157:Transcription Factors / Q000235:genetics / Q000502:physiology
chemical_list: D018797:Cell Cycle Proteins; D016213:Cyclins; D029721:Drosophila Proteins; D014157:Transcription Factors; D016203:CDC2 Protein Kinase; D010749:Phosphoprotein Phosphatases; D017027:Protein Tyrosine Phosphatases; C059217:stg protein, Drosophila