Streptococcus mitis cell walls and lipopolysaccharide induce lethality in D-galactosamine-sensitized mice by a tumor necrosis factor-dependent pathway.
Purified cells walls of Streptococcus mitis induced tumor necrosis factor in vitro in whole blood of both lipopolysaccharide (LPS)-sensitive OF1 and LPS-resistant C3H/HeJ mice. They were as effective as heat-killed bacteria in inducing death in both strains of mice sensitized with D-galactosamine. Lethality was suppressed by anti-tumor necrosis factor antibodies. The histopathophysiological findings in mice after challenge with LPS or gram-positive cell walls were indistinguishable.
Le Roy
D
D
Department of Medicine, CHUV-Lausanne, Switzerland.
Morand
P
P
Lengacher
S
S
Celio
M
M
Grau
G E
GE
Glauser
M P
MP
Heumann
D
D
D000818:Animals; D000942:Antigens, Bacterial / Q000008:administration & dosage; D002473:Cell Wall / Q000276:immunology*; D005688:Galactosamine / Q000276:immunology*; D007114:Immunization; D066298:In Vitro Techniques; D008070:Lipopolysaccharides / Q000633:toxicity*; D008099:Liver / Q000473:pathology; D051379:Mice; D008809:Mice, Inbred C3H; D009504:Neutrophils / Q000473:pathology / Q000502:physiology; D012772:Shock, Septic / Q000209:etiology; D013290:Streptococcal Infections / Q000209:etiology; D013291:Streptococcus / Q000276:immunology* / Q000472:pathogenicity*; D014409:Tumor Necrosis Factor-alpha / Q000502:physiology*; D014774:Virulence
D000942:Antigens, Bacterial; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D005688:Galactosamine