title: Shark-cartilage containing preparation protects cells against hydrogen peroxide induced damage and mutagenesis.
abstract: Natural products from flora and fauna are frequently used as nutritional supplements and medicaments. Two short-term assays were carried out and negative results were obtained for shark-cartilage containing preparation. The tests employed were the Salmonella/mammalian microsome assay using tester strains TA97, TA98, TA100, TA102 and TA1535 with or without S9 mix and the SOS-Chromotest with Escherichia coli strain PQ37. Evidence for shark-cartilage containing preparation functioning as an antimutagen was detected. Using bacterial survival assays with Escherichia coli fpg (BH20) and xthA (BW9091), we investigated the putative role of shark-cartilage containing preparation in protecting cells against lesions induced by hydrogen peroxide in normal and low iron level conditions. Our data suggest that shark-cartilage containing preparation can play a scavenger role for reactive oxygen species and protect against DNA lesions in both conditions.
authors.affiliation: CETOX-Departamento de Biofísica e Biometria, Universidade do Estado do Rio de Janeiro, Brazil.
authors.forename: E M
authors.initials: EM
authors.lastname: Gomes
authors.forename: P R
authors.initials: PR
authors.lastname: Souto
authors.forename: I
authors.initials: I
authors.lastname: Felzenszwalb
mesh_terms: D015082:2,2'-Dipyridyl / Q000494:pharmacology; D015112:4-Nitroquinoline-1-oxide / Q000378:metabolism / Q000633:toxicity; D000818:Animals; D016587:Antimutagenic Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology; D001711:Biotransformation; D002356:Cartilage / Q000737:chemistry*; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics; D016166:Free Radical Scavengers / Q000494:pharmacology; D006861:Hydrogen Peroxide / Q000378:metabolism / Q000633:toxicity*; D007501:Iron / Q000378:metabolism; D016296:Mutagenesis; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity; D017382:Reactive Oxygen Species / Q000378:metabolism; D013014:SOS Response, Genetics; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics; D012754:Sharks
chemical_list: D016587:Antimutagenic Agents; D000975:Antioxidants; D016166:Free Radical Scavengers; D009153:Mutagens; D017382:Reactive Oxygen Species; D015082:2,2'-Dipyridyl; D015112:4-Nitroquinoline-1-oxide; D006861:Hydrogen Peroxide; D007501:Iron