An Archaea 5S rRNA analog is stably expressed in Escherichia coli.
Mini-genes for 5S-like rRNA were constructed. These genes had a sequence which largely resembles that of the naturally occurring 5S rRNA of a bacterium, Halococcus morrhuae, which phylogenetically belongs to the Archaea. Plasmids carrying the mini-genes were transformed into Escherichia coli (Ec). Ribosomal incorporation was not a prerequisite for stable accumulation of the RNA product. However, only those constructs with a well-base-paired helix I accumulated RNA product. This result strongly implies that this aspect of the structure is likely to be an important condition for stabilizing 5S rRNA-like products. The results are consistent with our current understanding of 5S rRNA processing in Ec. When used in conjunction with rRNA probe technology, the resulting chimeric RNA may be useful as a monitoring tool for genetically engineered microorganisms or naturally occurring organisms that are released into the environment.
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Department of Biomedical and Biophysical Sciences, University of Houston, TX 77204-5934, USA.
Fox
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D001483:Base Sequence; D015152:Blotting, Northern; D015342:DNA Probes / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D004784:Environmental Monitoring / Q000379:methods; D004926:Escherichia coli / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics; D005818:Genetic Engineering; D005822:Genetic Vectors / Q000235:genetics; D006216:Halobacteriaceae / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed / Q000235:genetics; D009690:Nucleic Acid Conformation; D010802:Phylogeny; D015347:RNA Probes / Q000235:genetics; D012329:RNA, Bacterial / Q000096:biosynthesis / Q000235:genetics*; D012341:RNA, Ribosomal, 5S / Q000096:biosynthesis / Q000235:genetics*; D012270:Ribosomes / Q000378:metabolism; D014170:Transformation, Genetic / Q000235:genetics
D015342:DNA Probes; D015347:RNA Probes; D012329:RNA, Bacterial; D012341:RNA, Ribosomal, 5S