[Radiobiologic in-vivo investigation of angiogenesis in the yolk sac vascular system of chicken embryos].
OBJECTIVE
The aim of this study was to investigate whether the yolk sac blood vessel system of chick embryos represents a useful model of in-vivo measurements of angiogenesis in radiobiology.


METHODS
The fertilized eggs of the crossbreeding White Plymouth Rox X Sussex were used for the study of the extraembryonic vascularisation after irradiation with different fractionation and dosage. On day 3 of incubation an oval window was cut into the shell and then the area vasculosa was irradiated with 1.0 cm in diameter. Forty-eight hours after irradiation in-vivo photographs were taken for quantitative evaluation of blood vessel density.


RESULTS
Irradiation from 2.0 to 8.0 Gy in a single fraction leads to a slight decrease in vascular density. After a single fraction of 10.0 Gy a distinct increase in vascular density occurs. Fractionated irradiation leads to a considerable clear increasing of vascular density in comparison with the single fraction and same total dose.


CONCLUSIONS
Our model of the fertilized egg enables in-vivo measurements of angiogenesis in radiobiology.
Plasswilm
L
L
Klinik für Strahlentherapie, Universität Erlangen-Nürnberg.
Höper
J
J
Sauer
R
R
D000818:Animals; D002642:Chick Embryo; D005306:Fertilization; D008833:Microcirculation / Q000528:radiation effects; D018919:Neovascularization, Physiologic / Q000528:radiation effects*; D011829:Radiation Dosage; D015017:Yolk Sac / Q000098:blood supply*