Inhibition of 12-O-tetradecanoylphorbol-13-acetate promoted mouse skin papilloma by digalactosyl diacylglycerols from the fresh water cyanobacterium Phormidium tenue.
To search for possible antitumor-promoters, two digalactosyl diacylglycerols (DGDGs), which were obtained from the freshwater cyanobacterium Phormidium tenue and possessed a single pair of acyl residues, were evaluated for their inhibitory effects on the two-stage carcinogenesis test in mouse skin. Papillomas in mouse skin were initiated with 390 nmol of 7,12-O-dimethylbenz[a]anthracene and 1 week later, were promoted twice a week with 1.7 nmol of 12-O-tetradecanoylphorbol-13-acetate (TPA). Two DGDGs effectively inhibited tumor formation in the sensitive mouse stock even when these compounds were given 1 h before TPA treatment.
Tokuda
H
H
Department of Biochemistry, Kyoto Prefectural University of Medicine, Japan.
Nishino
H
H
Shirahashi
H
H
Murakami
N
N
Nagatsu
A
A
Sakakibara
J
J
D015127:9,10-Dimethyl-1,2-benzanthracene / Q000633:toxicity; D000287:Administration, Topical; D000818:Animals; D016588:Anticarcinogenic Agents / Q000302:isolation & purification / Q000627:therapeutic use*; D002240:Carbohydrate Sequence; D015197:Carcinogenicity Tests; D002273:Carcinogens / Q000633:toxicity*; D000458:Cyanobacteria / Q000737:chemistry*; D005260:Female; D038983:Galactolipids; D006017:Glycolipids / Q000302:isolation & purification / Q000627:therapeutic use*; D051379:Mice; D008813:Mice, Inbred ICR; D008969:Molecular Sequence Data; D010212:Papilloma / Q000139:chemically induced* / Q000517:prevention & control*; D012878:Skin Neoplasms / Q000139:chemically induced* / Q000517:prevention & control*; D013755:Tetradecanoylphorbol Acetate / Q000037:antagonists & inhibitors* / Q000633:toxicity
D016588:Anticarcinogenic Agents; D002273:Carcinogens; D038983:Galactolipids; D006017:Glycolipids; C007388:digalactosyldiacylglycerol; D015127:9,10-Dimethyl-1,2-benzanthracene; D013755:Tetradecanoylphorbol Acetate