title: Binding of 2',3'-cyclic nucleotide 3'-phosphodiesterase to myelin: an in vitro study.
abstract: The binding of 2', 3'-cyclic nucleotide 3'-phosphodiesterase isoform 1 (CNP1) to myelin and its association with cytoskeletal elements of the sheath have been characterized with in vitro synthesized polypeptides and purified myelin. We have previously shown that the cysteine residue present in the carboxy-terminal CXXX box of CNP1 is isoprenylated, and that both C15 farnesyl and C20 geranylgeranyl isoprenoids can serve as substrates for the modification. Here, we have mutated the CXXX box to obtain selectively farnesylated CNP1 or geranyl- geranylated CNP1 and found that these two modified forms of CNP1 behave identically in all of the assays performed. Isoprenylation is essential but not sufficient for the binding of in vitro synthesized CNP1 to purified myelin, because a control nonmyelin protein is isoprenylated, yet unable to bind to myelin. In our assay, membrane-bound CNP1 partitions quantitatively into the nonionic detergent-insoluble phase of myelin, suggesting that CNP1 binds to cytoskeletal elements within myelin. However, isoprenylated CNP1 fails to bind to the cytoskeletal matrix isolated from myelin by detergent treatment, implying that both detergent-soluble and insoluble myelin components are involved in the binding of CNP1. A model for the interactions between CNP1 and myelin is presented, consistent with models proposed for other isoprenylated proteins.
authors.affiliation: Biochemistry Department, McGill University, Montreal, Quebec, Canada.
authors.forename: D A
authors.initials: DA
authors.lastname: De Angelis
authors.forename: P E
authors.initials: PE
authors.lastname: Braun
mesh_terms: D063309:2',3'-Cyclic Nucleotide 3'-Phosphodiesterase; D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases / Q000737:chemistry* / Q000378:metabolism; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D005347:Fibroblasts / Q000201:enzymology; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D009185:Myelin Proteins / Q000737:chemistry* / Q000378:metabolism; D010727:Phosphoric Diester Hydrolases; D011485:Protein Binding; D017368:Protein Prenylation; D014162:Transfection
chemical_list: D009185:Myelin Proteins; D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases; D010727:Phosphoric Diester Hydrolases; D063309:2',3'-Cyclic Nucleotide 3'-Phosphodiesterase