Involvement of the oxidative pentose phosphate pathway in thiamine biosynthesis in Salmonella typhimurium.
purF mutants of Salmonella typhimurium are known to require a source of both purine and thiamine; however, exogenous pantothenate may be substituted for the thiamine requirement. We show here that the effect of pantothenate is prevented by blocks in the oxidative pentose phosphate pathway, gnd (encoding gluconate 6-phosphate [6-P] dehydrogenase) or zwf (encoding glucose 6-P dehydrogenase). We further show that the defects caused by these mutations can be overcome by increasing ribose 5-P, suggesting that ribose 5-P may play a role in the ability of pantothenate to substitute for thiamine.
Enos-Berlage
J L
JL
Department of Bacteriology, University of Wisconsin--Madison 53706, USA.
Downs
D M
DM
D000332:Aerobiosis; D000693:Anaerobiosis; D005954:Glucosephosphate Dehydrogenase / Q000235:genetics / Q000378:metabolism; D008954:Models, Biological; D009154:Mutation; D010084:Oxidation-Reduction; D010205:Pantothenic Acid / Q000378:metabolism; D010427:Pentose Phosphate Pathway; D010734:Phosphogluconate Dehydrogenase / Q000235:genetics / Q000378:metabolism; D011743:Pyrimidines / Q000378:metabolism; D012267:Ribosemonophosphates / Q000378:metabolism; D012486:Salmonella typhimurium / Q000235:genetics / Q000378:metabolism*; D013831:Thiamine / Q000096:biosynthesis*
D011743:Pyrimidines; D012267:Ribosemonophosphates; D010205:Pantothenic Acid; C031626:ribose-5-phosphate; D010734:Phosphogluconate Dehydrogenase; C029757:phosphogluconate dehydrogenase (decarboxylating); D005954:Glucosephosphate Dehydrogenase; D013831:Thiamine