Human herpesvirus-8 DNA sequences in human immunodeficiency virus-negative angioimmunoblastic lymphadenopathy and benign lymphadenopathy with giant germinal center hyperplasia and increased vascularity.
Human herpesvirus-8 (HHV-8) DNA sequences have been reported to be strictly associated not only with various forms of Kaposi's sarcoma but also with an unusual subgroup of acquired immunodeficiency syndrome (AIDS)-related B-cell lymphomas. A possible relation of this putative virus also with multicentric Castleman's disease (MCD) has been recently suggested. We used polymerase chain reaction to look for the presence of HHV-8 sequences in a well characterized series of benign, atypical, and malignant lymphoid tissues from 45 Hodgkin's disease and 43 non-Hodgkin's lymphoma (NHL) cases, as well as from 5 MCD, 15 angioimmunoblastic lymphadenopathy (AILD), and 23 benign lymphadenopathy cases. Among the 38 AIDS-related lymphoid lesions, only 1 NHL and 1 persistent generalized lymphadenopathy (PGL) case were positive. Furthermore, among the 92 non-AIDS-related lymphoproliferative disorders, HHV-8 sequences were detected in 3 classic AILD cases and in 4 reactive lymphadenopathies. Six of 9 HHV-8 positive lymphoid lesions (1 NHL, 1 PGL, 1 AILD, and 3 reactive lymphadenopathy cases) were also positive for Epstein-Barr viral sequences. The four human immunodeficiency virus (HIV) negative lymphadenopathies positive for HHV-8 sequences showed an almost identical histology, characterized by a predominantly follicular lesion, with giant germinal center hyperplasia, and increased vascularity, resembling HIV-related lymphadenopathy and MCD. Our results, while providing the first evidence of the presence of HHV-8 sequences in AILD cases, suggest a possible association of these herpes viral sequences with a distinct histologic type of non-neoplastic lymphadenopathy, not associated with other common herpes infections.
Luppi
M
M
Department of Medical Sciences, University of Modena, Italy.
Barozzi
P
P
Maiorana
A
A
Artusi
T
T
Trovato
R
R
Marasca
R
R
Savarino
M
M
Ceccherini-Nelli
L
L
Torelli
G
G
D001483:Base Sequence; D004279:DNA, Viral / Q000032:analysis; D006564:Herpesviridae / Q000302:isolation & purification*; D006689:Hodgkin Disease / Q000473:pathology / Q000821:virology*; D006801:Humans; D006965:Hyperplasia; D007119:Immunoblastic Lymphadenopathy / Q000473:pathology / Q000821:virology*; D008198:Lymph Nodes / Q000098:blood supply / Q000473:pathology; D016483:Lymphoma, AIDS-Related / Q000473:pathology / Q000821:virology*; D008228:Lymphoma, Non-Hodgkin / Q000473:pathology / Q000821:virology*; D008969:Molecular Sequence Data; D009389:Neovascularization, Pathologic; D017421:Sequence Analysis
D004279:DNA, Viral