title: Characterization of the membranous denitrification enzymes nitrite reductase (cytochrome cd1) and copper-containing nitrous oxide reductase from Thiobacillus denitrificans.
abstract: Cytochrome cd1-nitrite reductase and nitrous oxide reductase of Thiobacillus denitrificans were purified and characterized by biochemical and immunochemical methods. In contrast to the generally soluble nature of the denitrification enzymes, these two enzymes were isolated from the membrane fraction of T. denitrificans and remained active after solubilization with Triton X-100. The properties of the membrane-derived enzymes were similar to those of their soluble counterparts from the same organism. Nitrous oxide reductase activity was inhibited by acetylene. Nitrite reductase and nitrous oxide reductase cross-reacted with antisera raised against the soluble enzymes from Pseudomonas stutzeri. The nirS, norBC, and nosZ genes encoding the cytochrome cd1-nitrite reductase, nitric oxide reductase, and nitrous oxide reductase, respectively, from P. stutzeri hybridized with genomic DNA from T. denitrificans. Cross-reactivity and similar N-terminal amino acid and gene sequences suggest that the primary structures of the Thiobacillus enzymes are homologous to the soluble proteins from P. stutzeri.
authors.affiliation: Fakultät fur Biologie, Universität Konstanz, Germany.
authors.forename: U H
authors.initials: UH
authors.lastname: Hole
authors.forename: K U
authors.initials: KU
authors.lastname: Vollack
authors.forename: W G
authors.initials: WG
authors.lastname: Zumft
authors.forename: E
authors.initials: E
authors.lastname: Eisenmann
authors.forename: R A
authors.initials: RA
authors.lastname: Siddiqui
authors.forename: B
authors.initials: B
authors.lastname: Friedrich
authors.forename: P M
authors.initials: PM
authors.lastname: Kroneck
mesh_terms: D000595:Amino Acid Sequence; D015139:Blotting, Southern; D008969:Molecular Sequence Data; D009572:Nitrite Reductases / Q000302:isolation & purification / Q000378:metabolism*; D010088:Oxidoreductases / Q000302:isolation & purification / Q000378:metabolism*; D013855:Thiobacillus / Q000201:enzymology*
chemical_list: D010088:Oxidoreductases; D009572:Nitrite Reductases; C022763:nitrous oxide reductase