[New technique for implanting Bomirski melanoma into the anterior chamber of Syrian hamster eyes].
OBJECTIVE
To describe a newly elaborated ocular tumor model, obtained by implanting Bomirski melanoma into the anterior chamber of the eye in Syrian hamsters (Mesocricetus auratus).


METHODS
The experiments were carried out using pigmented and non-pigmented lines of the tumor. Attempted injections of suspension of malignant melanoma cells in physiological salt solution to the anterior chamber with an infusion pump did not give the expected results. The growth of the tumor occurred only in 8% of eyes. The research material contained 132 adult pigmented hamsters. Tumor fragments of the size of 0,4-1,0 mm were implanted to the anterior chamber of the eyes. Melanotic cells were implanted in 71 hamsters, amelanotic ones in 61 hamsters. Hamsters were anesthetized with intravisceral vetbutal. The examination was carried out in 24 hour intervals.


RESULTS
During the first 2 to 3 days the disappearance of the cells were observed, and then iris tumors appeared, in the cases of amelanotic cells after 4-6 days, and in the cases of melanotic cells after 8-10 days. Enucleated eye-balls were examined histopathologically. In all cases confirmation of the growth of melanoma in the iris was obtained.
Romanowska
B
B
Katedry i Kliniki Okulistyki Collegium Medicum Uniwersytetu Jagiellońskiego w Krakowie.
Kukiełczak
B
B
Bryk
J
J
Mirkiewicz-Sieradzka
B
B
Heitzmann
J
J
Lukiewicz
S
S
D000818:Animals; D000867:Anterior Chamber; D002455:Cell Division; D006224:Cricetinae; D004195:Disease Models, Animal; D015811:Iris Neoplasms / Q000473:pathology; D008546:Melanoma, Experimental / Q000473:pathology; D009368:Neoplasm Transplantation / Q000379:methods*