Arachidonic acid metabolism and cell proliferation in rat mammary carcinoma cells treated with indomethacin.
To determine the specificity of action of indomethacin as an inhibitor of cyclooxygenase in the mammary epithelium, cell proliferation and levels of PGE2 and LTB4 were quantitated in 13762 MAT rat mammary carcinoma cells treated with 10(-4) to 10(-10) M concentrations of drug. Suppression of proliferation of 13762 MAT cells by indomethacin was associated with reduced levels of both PGE2 and LTB4. The antiproliferative activity of indomethacin in rat mammary carcinoma cells may be modulated through inhibition of both cyclooxygenase and lipoxygenase pathways of arachidonic acid metabolism.
Tripathi
S C
SC
Life Sciences Department, IIT Research Institute, Chicago, Illinois 60616, USA.
Vosseller
K A
KA
McCormick
D L
DL
D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism*; D002455:Cell Division / Q000187:drug effects*; D015232:Dinoprostone / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D007213:Indomethacin / Q000494:pharmacology*; D007975:Leukotriene B4 / Q000378:metabolism; D008325:Mammary Neoplasms, Experimental / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D014407:Tumor Cells, Cultured
D007975:Leukotriene B4; D016718:Arachidonic Acid; D015232:Dinoprostone; D007213:Indomethacin