title: Argon laser induced autofluorescence may distinguish between normal and tumor human urothelial cells: a microspectrofluorimetric study.
abstract: OBJECTIVE
  To assess the ability of argon laser-induced autofluorescence spectroscopy (LIAFS) to discriminate normal from tumor human urothelial cells.
  
  
  METHODS
  Emission spectra of single living cells excited at 488 nm. have been studied with confocal microspectrofluorimeter.
  
  
  RESULTS
  Cellular autofluorescence appeared as a broad band with a maximum in the same "green" spectral range, 550 to 560 nm., probably corresponding to oxidized flavoprotein emission. However, the maximum autofluorescence intensity of normal urothelial cells was much higher, 10 times (p<0.0001) that of any of the tumor cell types tested.
  
  
  CONCLUSIONS
  These results, suggesting a significantly reduced oxidized flavoprotein concentration in tumor urothelial cells, should prompt us to evaluate argon LIAFS as a potential tool to detect occult urothelial severe dysplasia and carcinoma in situ.
authors.affiliation: Departement d'Urologie, Hopital Saint-Louis, Paris, France.
authors.forename: M
authors.initials: M
authors.lastname: Anidjar
authors.forename: O
authors.initials: O
authors.lastname: Cussenot
authors.forename: J
authors.initials: J
authors.lastname: Blais
authors.forename: O
authors.initials: O
authors.lastname: Bourdon
authors.forename: S
authors.initials: S
authors.lastname: Avrillier
authors.forename: D
authors.initials: D
authors.lastname: Ettori
authors.forename: J M
authors.initials: JM
authors.lastname: Villette
authors.forename: J
authors.initials: J
authors.lastname: Fiet
authors.forename: P
authors.initials: P
authors.lastname: Teillac
authors.forename: A
authors.initials: A
authors.lastname: Le Duc
mesh_terms: D001128:Argon; D002295:Carcinoma, Transitional Cell / Q000737:chemistry / Q000175:diagnosis* / Q000473:pathology; D002478:Cells, Cultured; D003937:Diagnosis, Differential; D005420:Flavoproteins / Q000032:analysis; D006801:Humans; D007834:Lasers; D013050:Spectrometry, Fluorescence; D001743:Urinary Bladder / Q000166:cytology*; D001749:Urinary Bladder Neoplasms / Q000737:chemistry / Q000175:diagnosis* / Q000473:pathology
chemical_list: D005420:Flavoproteins; D001128:Argon