Analysis of regulatory elements of the developmentally controlled chorion s15 promoter in transgenic Drosophila.
The Drosophila s15 chorion gene is expressed only in the follicular epithelium surrounding the developing oocyte, with tight quantitative control and a very narrow temporal specificity. We have used germ-line transformation analysis to conduct an extensive mutational dissection of its promoter between -189 and -39 bp relative to the transcriptional start site. Quantitative control and temporal specificity are disrupted by several of these mutations. The results suggest that this 150-bp DNA region encompasses many positive and negative, at least partially degenerate, cis-regulatory elements, which are involved in specifying the highly precise expression pattern of the s15 gene during development.
Mariani
B D
BD
Department of Biochemistry and Molecular Biology, Thomas Jefferson University, Philadelphia, PA 19107-5099, USA.
Shea
M J
MJ
Conboy
M J
MJ
Conboy
I
I
King
D L
DL
Kafatos
F C
FC
D000595:Amino Acid Sequence; D000818:Animals; D030801:Animals, Genetically Modified; D001483:Base Sequence; D004331:Drosophila melanogaster / Q000196:embryology / Q000235:genetics*; D004527:Egg Proteins / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D017344:Genes, Insect; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics; D016415:Sequence Alignment; D017384:Sequence Deletion; D017386:Sequence Homology, Amino Acid
D004527:Egg Proteins; D012333:RNA, Messenger