cDNAs encoding spinach stromal and thylakoid-bound ascorbate peroxidase, differing in the presence or absence of their 3'-coding regions.
Two cDNA clones encoding stromal (SAP28) and thylakoid-bound (SAP22) ascorbate peroxidase were isolated from a spinach cDNA library constructed by greening cotyledons. The SAP22 and SAP28 contained an open reading frame encoding mature protein of 295 and 345 amino acids with calculated molecular mass of 32239 Da and 37710 Da, respectively, preceded by the common transit peptides of 70 amino acid residues. Interestingly, the N-terminal 364 amino acids of SAP22 were 100% identical with SAP28 except for one C-terminal amino acid residue (Asp-365), and the C-terminal of SAP22, which is the putative transmembrane segment, was 50 amino acids longer than that of SAP28.
Ishikawa
T
T
Department of Food and Nutrition, Faculty of Agriculture, Kinki University, Nara, Japan.
Sakai
K
K
Yoshimura
K
K
Takeda
T
T
Shigeoka
S
S
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D060387:Ascorbate Peroxidases; D001483:Base Sequence; D001665:Binding Sites; D002462:Cell Membrane / Q000378:metabolism; D002736:Chloroplasts / Q000648:ultrastructure; D003001:Cloning, Molecular / Q000379:methods; D017124:Conserved Sequence; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005055:Euglena / Q000276:immunology; D007527:Isoenzymes / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010544:Peroxidases / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism*; D016133:Polymerase Chain Reaction / Q000379:methods; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D018724:Spinacia oleracea / Q000201:enzymology* / Q000235:genetics*
D000911:Antibodies, Monoclonal; D018076:DNA, Complementary; D007527:Isoenzymes; D011994:Recombinant Proteins; D010544:Peroxidases; D060387:Ascorbate Peroxidases