Flagellar genes from Rhodobacter sphaeroides are homologous to genes of the fliF operon of Salmonella typhimurium and to the type-III secretion system.
A flagellar region of the genome of Rhodobacter sphaeroides was cloned and sequenced. Three ORFs were identified and arranged in the same order as fliH, fliI and fliJ of Salmonella typhimurium (St). ORF2 is highly similar to FliI from St (49% similarity) showing Walker's A and B motifs. Similar scores were found with proteins of the type-III secretion system of virulence factors. ORF3 shows 16.4 and 11.1% similarity to FliJ from St and Bacillus subtilis, respectively. This work also shows that ORF3 is similar to HrpJ5 from Pseudomonas syringae (19.2% similarity). It was found that ORF2 and ORF3 start immediately downstream from the adjacent coding region, suggesting a single transcriptional unit.
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Departamento de Bioenergética, Universidad Nacional Autónoma de México, D.F., México.
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Dreyfus
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D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D005407:Flagella / Q000378:metabolism*; D005798:Genes, Bacterial; D008565:Membrane Proteins; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics*; D006180:Proton-Translocating ATPases; D012242:Rhodobacter sphaeroides / Q000235:genetics* / Q000472:pathogenicity; D012486:Salmonella typhimurium / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D014774:Virulence / Q000235:genetics
D001426:Bacterial Proteins; C075661:Flif protein, Bacteria; D008565:Membrane Proteins; D011506:Proteins; C069250:fliH protein, Bacteria; C069251:fliI protein, bacteria; C069253:fliJ protein, Bacteria; D006180:Proton-Translocating ATPases