title: Mice deficient for the amyloid precursor protein gene.
abstract: To understand the in vivo function of the amyloid precursor protein (APP) we generated an APP null mutation in mice by homologous recombination in embryonic stem (ES) cells. We show here that homozygous APP deficient mice were produced at expected frequencies. Neither APP mRNA nor protein could be detected in these animals. Yet the homozygous APP mutant mice are fertile and do not show overt abnormalities at up to 12 weeks of age. Neuroanatomical studies of the brain did not reveal significant differences in the knockout mice as compared to the wild-type controls. These results argue against an essential function of APP in mouse embryonic and early neuronal development.
authors.affiliation: Department of Genetics & Molecular Biology, Merck Research Laboratories, Merck & Co. Inc., Rahway, New Jersey 07065, USA.
authors.forename: H
authors.initials: H
authors.lastname: Zheng
authors.forename: M
authors.initials: M
authors.lastname: Jiang
authors.forename: M E
authors.initials: ME
authors.lastname: Trumbauer
authors.forename: R
authors.initials: R
authors.lastname: Hopkins
authors.forename: D J
authors.initials: DJ
authors.lastname: Sirinathsinghji
authors.forename: K A
authors.initials: KA
authors.lastname: Stevens
authors.forename: M W
authors.initials: MW
authors.lastname: Conner
authors.forename: H H
authors.initials: HH
authors.lastname: Slunt
authors.forename: S S
authors.initials: SS
authors.lastname: Sisodia
authors.forename: H Y
authors.initials: HY
authors.lastname: Chen
authors.forename: L H
authors.initials: LH
authors.lastname: Van der Ploeg
mesh_terms: D016564:Amyloid beta-Protein Precursor / Q000235:genetics*; D000818:Animals; D001921:Brain / Q000378:metabolism; D017353:Gene Deletion; D005822:Genetic Vectors; D006579:Heterozygote; D006720:Homozygote; D007668:Kidney / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D016564:Amyloid beta-Protein Precursor; D012333:RNA, Messenger