Physiological responses to repeated endotoxin challenge are selectively affected by recombinant bovine somatotropin administration to calves.
The study determined 1) whether the pretreatment of calves with recombinant bovine somatotropin (bST, sometribove) would alter the change in packed cell volume (PCV), rectal temperature (RT), and the plasma concentrations of Ca2+, Fe2+, glucose (G), urea N (PUN), nonesterified free fatty acids (NEFA), albumin (ALB), and blood cell populations after endotoxin challenge (EC) and 2) whether the natural development of physiologic tolerance to repeated EC was affected by bST. Twelve steer beef calves were assigned to either control (-bST) or +bST treatment in equal numbers. Calves were injected intramuscularly with either HCO3(-)-buffered saline or bST (0.1 mg/kg) daily for 5 d. On Day 6, the first EC was administered (Escherichia coli, 055:B5, 0.2 microgram/kg, intravenous bolus in pyrogen-free saline). Saline or bST injections were continued from Day 7 up to the repeat of EC on Day 11. RT and PCV were measured hourly through 12 and 6 hr, respectively. Jugular blood was obtained at 0, +1, 2, 3, 4, 6, 8, 12, and 24 hr relative to each EC. bST had no effect on the increase in RT, the hyperglycemic phase of the G response, the biphasic change in Fe2+, or increases in NEFA and PUN. PCV increased after each EC only in -bST. The mean decrease in G during the hypoglycemic phase was less in +bST. Hypocalcemic responses were significantly less in +bST. ALB concentrations decreased after each challenge; the response was unaffected by bST. CD2+, CD4+, and CD8+ T-lymphocyte populations were unaffected by bST and EC. Overall, the magnitude of change in all plasma variables was less after the second EC compared with the first, either in terms of magnitude or duration. The data suggest that the treatment of calves with bST diminishes the magnitude of hypoglycemic, hypocalcemic, and PCV changes after EC and does not compromise fever response, changes in blood cell populations, or tolerance to repeated EC.
Elsasser
T H
TH
Growth Biology Laboratory, U.S. Department of Agriculture, Beltsville, MD 20705, USA.
Richards
M
M
Collier
R
R
Hartnell
G F
GF
D000818:Animals; D001772:Blood Cell Count; D001786:Blood Glucose / Q000032:analysis; D001831:Body Temperature / Q000187:drug effects / Q000502:physiology; D002118:Calcium / Q000097:blood; D002417:Cattle / Q000097:blood* / Q000502:physiology; D004347:Drug Interactions; D004731:Endotoxins / Q000633:toxicity*; D004926:Escherichia coli / Q000502:physiology; D005230:Fatty Acids, Nonesterified / Q000097:blood; D013006:Growth Hormone / Q000494:pharmacology*; D007501:Iron / Q000097:blood; D008297:Male; D011994:Recombinant Proteins / Q000494:pharmacology; D012709:Serum Albumin / Q000032:analysis; D016176:T-Lymphocyte Subsets / Q000166:cytology; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis*; D014508:Urea / Q000097:blood
D001786:Blood Glucose; D004731:Endotoxins; D005230:Fatty Acids, Nonesterified; D011994:Recombinant Proteins; D012709:Serum Albumin; D014409:Tumor Necrosis Factor-alpha; D014508:Urea; D013006:Growth Hormone; D007501:Iron; C108583:sometribove; D002118:Calcium