Ability of paralyzed flagella mutants of Chlamydomonas to move.
Chlamydomonas mutants missing the central pair or radial spokes are paralyzed despite the fact that they have the full wild-type complement of functional dynein ATPases. We show here that these mutants can move under conditions of low ATP concentration, a combination of ATP and ADP, and a combination of ATP and ribose-modified ATP analogs. These conditions suggest an inhibitory role of ATP and that this inhibition can be relieved by ADP or analogs. The function of the central-pair/radial spoke complex may be to release this ATP inhibition in a controlled manner.
Omoto
C K
CK
Department of Genetics and Cell Biology, Washington State University, Pullman 99164-4234, USA.
Yagi
T
T
Kurimoto
E
E
Kamiya
R
R
D000244:Adenosine Diphosphate / Q000031:analogs & derivatives / Q000378:metabolism / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000031:analogs & derivatives / Q000378:metabolism / Q000494:pharmacology; D000818:Animals; D002465:Cell Movement / Q000502:physiology*; D002696:Chlamydomonas / Q000166:cytology* / Q000235:genetics / Q000648:ultrastructure; D004398:Dyneins / Q000235:genetics / Q000378:metabolism; D005407:Flagella / Q000737:chemistry / Q000502:physiology*; D018715:Microscopy, Video; D009154:Mutation / Q000502:physiology
D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D004398:Dyneins