title: The insE open reading frame of IS1 is not required for formation of cointegrates.
abstract: The role of the insE open reading frame in transposition of IS1 was reexamined by using an insE nonsense mutation that does not alter the amino acid sequence of InsA inhibitor or InsAB transposase. The mutant was active in all strains tested, showing that insE is not essential for formation of cointegrates.
authors.affiliation: Hedco Molecular Biology Laboratories, University of Southern California, Los Angeles 90089-1340, USA.
authors.forename: E T
authors.initials: ET
authors.lastname: Freund
authors.forename: M M
authors.initials: MM
authors.lastname: Susskind
mesh_terms: D004251:DNA Transposable Elements / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics*; D004755:Enterobacteriaceae / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D016254:Mutagenesis, Insertional; D016366:Open Reading Frames; D012486:Salmonella typhimurium / Q000235:genetics
chemical_list: D004251:DNA Transposable Elements; D004269:DNA, Bacterial