title: Identification of a HeLa mRNA fraction which corrects the mitomycin C sensitivity of irs1 cells.
abstract: The hamster cell mutant irs1 is defective in its response to DNA lesions caused by a variety of mutagens, particularly cross-linking agents. These cells have been assigned to complementation group 2 of X-ray-sensitive mutants and the mutated gene is called XRCC2(X-ray repair cross complementing). We have identified, by microinjection, a human mRNA fraction which can transiently correct the sensitivity of these cells to cross-linking agents. This fraction contains mRNAs of 3.5 kb (+/- 0.25) including, therefore, the transcript of the XRCC2 gene.
authors.affiliation: Institut für Humangenetik, Virchow Klinikum, Humboldt Universität zu Berlin, Germany.
authors.forename: O
authors.initials: O
authors.lastname: Bender
authors.forename: N J
authors.initials: NJ
authors.lastname: Jones
authors.forename: K
authors.initials: K
authors.lastname: Sperling
authors.forename: M
authors.initials: M
authors.lastname: Digweed
mesh_terms: D000818:Animals; D002470:Cell Survival; D006224:Cricetinae; D004260:DNA Repair / Q000235:genetics*; D004305:Dose-Response Relationship, Drug; D005816:Genetic Complementation Test; D006367:HeLa Cells; D006801:Humans; D008845:Microinjections; D016685:Mitomycin / Q000494:pharmacology*; D009153:Mutagens / Q000494:pharmacology; D012333:RNA, Messenger / Q000302:isolation & purification*; D011836:Radiation Tolerance / Q000235:genetics
chemical_list: D009153:Mutagens; D012333:RNA, Messenger; D016685:Mitomycin