Hybrid proteins between Pseudomonas aeruginosa exotoxin A and poliovirus 2Apro cleave p220 in HeLa cells.
Cleavage of p220, a component of the initiation factor eIF-4F, has been correlated with the inhibition of host translation during poliovirus infection. To obtain p220 cleavage in the absence of any other poliovirus gene products, hybrid proteins containing Pseudomonas aeruginosa exotoxin A and poliovirus protease 2Apro have been constructed. The addition of the hybrid molecules to cultured cells did not lead to substantial p220 cleavage. However, the simultaneous presence of the hybrid toxin with replicationally inactive chicken adenovirus particles results in efficient cleavage of p220 in the intact cells. Under these conditions, cellular translation continues unabated for several hours, arguing against a direct requirement for intact p220 in each round of the initiation of translation of cellular mRNAs.
Novoa
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I
Centro de Biología Molecular, UAM-Consejo Superior de Investigaciones Científicas, Universidad Autónoma de Madrid, Spain.
Cotten
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Carrasco
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D036002:ADP Ribose Transferases; D001427:Bacterial Toxins; D003546:Cysteine Endopeptidases / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D039562:Eukaryotic Initiation Factor-4F; D005098:Exotoxins / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D046911:Macromolecular Substances; D010448:Peptide Initiation Factors / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D017955:Poliovirus / Q000378:metabolism; D014176:Protein Biosynthesis; D011550:Pseudomonas aeruginosa; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism; D014764:Viral Proteins; D037521:Virulence Factors; D000097668:Pseudomonas aeruginosa Exotoxin A
D036002:ADP Ribose Transferases; D001427:Bacterial Toxins; D003546:Cysteine Endopeptidases; D039562:Eukaryotic Initiation Factor-4F; D005098:Exotoxins; D046911:Macromolecular Substances; D010448:Peptide Initiation Factors; D011993:Recombinant Fusion Proteins; D014764:Viral Proteins; D037521:Virulence Factors; D000097668:Pseudomonas aeruginosa Exotoxin A; C057400:picornain 2A, Picornavirus