Use of culture-filtrated antigen in an ELISA and a dot immunoassay for the diagnosis of melioidosis.
An enzyme-linked immunosorbent assay (ELISA) and a dot immunoassay with culture-filtrated antigen were developed for detection of Burkholderia pseudomallei specific antibodies in melioidosis patients. Sixty-eight sera of bacteriologically confirmed melioidosis patients, 45 sera of other bacterial infected patients and 80 sera of healthy blood donors from endemic area were investigated. The samples were subjected to those assays im comparison with indirect hemagglutination (IHA). The sensitivity, specificity, positive and negative predictive values in this dot immunoassay were 94.1%, 99.2%, 98.5% and 96.9%, respectively, with cut-off dilution at 1:4,000, whereas those in ELISA were 92.6%, 96.8%, 94.0% and 96.0%, respectively, with cut-off value of OD = 0.47 at 490 nm. Meanwhile, those in IHA were 64.7%, 93.6%, 84.6%, 83.0% respectively, with a cut-off value of > or = 1:80. The results in this study demonstrated that the dot immunoassay was more reliable and rapid than ELISA as the serological test for diagnosis of melioidosis.
Wongratanacheewin
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S
Department of Microbiology, Faculty of Medicine, Khon Kaen University, Thailand. sura-wng@kkul.kku.ac.th
Amornpunt
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S
Sermswan
R W
RW
Tattawasart
U
U
Wongwajana
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S
D000942:Antigens, Bacterial / Q000276:immunology; D016022:Case-Control Studies; D004797:Enzyme-Linked Immunosorbent Assay; D006386:Hemagglutination Tests; D006801:Humans; D015151:Immunoblotting; D008554:Melioidosis / Q000175:diagnosis*; D012680:Sensitivity and Specificity; D013785:Thailand
D000942:Antigens, Bacterial