Reduced humoral immunity in uremic mice genetically selected for high antibody response.
Biozzi's Selection IV-A mice, genetically selected for 25 generations for high and low antibody response to sheep red blood cells (SRBC), 2-3 months old, were made uremic by subtotal nephrectomy and characterized for antibody production against the selection antigen. T cell activity was evaluated in vitro by lymphocyte proliferation and interleukin 2 (IL 2) production in response to the super antigen staphylococcal enterotoxin B (SEB). Total and IgM antibody titers (log2) were similar in uremic and non-uremic low responder mice (total antibody: 4.0 +/- 0.6 vs 3.6 +/- 0.6; IgG: 3.0 +/- 0.7 vs 2.4 +/- 0.4), while uremic high responders presented with non-uremic animals (total antibody: 10.8 +/- 1.6 vs 13.0 +/- 0.2; IgG: 10.3 +/- 1.5 vs 11.7 +/- 0.3). T cell proliferation and IL 2 production were similar in uremic and non-uremic groups after SEB stimulation. The results indicate a genetic effect on sensitivity to humoral immune response modulation by uremic status, with deficient antibody production despite a normal T cell proliferative response to mitogen stimulation in short-duration renal failure in high responder mice.
Prado
E B
EB
Laboratório de Fisiopatologia Renal, Faculdade de Medicina, Universidade de São Paulo, Brasil.
Cabrera
W H
WH
Nicastri
A L
AL
Prado
M J
MJ
Léo
P
P
Marcondes
M
M
Siqueira
M
M
Ibañez
O C
OC
D000818:Animals; D000917:Antibody Formation / Q000235:genetics*; D051379:Mice; D008815:Mice, Inbred Strains; D013601:T-Lymphocytes / Q000502:physiology*; D014511:Uremia / Q000276:immunology*