Characterization of p53 gene mutations in basal-cell carcinomas: comparison between sun-exposed and less-exposed skin areas.
Mutations in the p53 gene in 32 basal-cell carcinomas (BCC) developed in Japanese patients were identified by the polymerase chain reaction and single-strand-conformation polymorphism analysis, followed by sequencing the DNA. Among 16 BCC developed in continuously sun-exposed areas, 6 tumors showed 7 base substitutions, most of which were G:C to A:T transitions, mainly at the dipyrimidine sites. Seven out of 16 BCC developed in less-exposed areas showed 8 base substitutions, but the majority (75.0%) of them were transversions. These results suggest that the mutation in the p53 gene plays a significant role in the tumorigenesis of BCC developed in less-exposed areas as well as those in sun-exposed areas in Japanese patients. There must be therefore causative factors other than UV irradiation for BCC in less-exposed areas.
Matsumura
Y
Y
Department of Radiation Genetics, Faculty of Medicine, Kyoto University, Japan.
Nishigori
C
C
Yagi
T
T
Imamura
S
S
Takebe
H
H
D000368:Aged; D000369:Aged, 80 and over; D001483:Base Sequence; D002280:Basal Cell Carcinoma / Q000209:etiology* / Q000235:genetics*; D005260:Female; D016158:Genes, p53 / Q000528:radiation effects*; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009154:Mutation; D009381:Neoplasms, Radiation-Induced / Q000209:etiology* / Q000235:genetics*; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D012867:Skin / Q000528:radiation effects; D012878:Skin Neoplasms / Q000209:etiology* / Q000235:genetics*; D013472:Sunlight / Q000009:adverse effects*