Molecular cloning and characterization of a novel stromal cell-derived cDNA encoding a protein that facilitates gene activation of recombination activating gene (RAG)-1 in human lymphoid progenitors.
The activation and expression of recombination activation genes (RAGs) in lymphoid progenitors are regulated by signals from surface molecules of stromal cells and/or cytokines. Using a mRNA differential display method, we isolated a novel stromal cell-derived cDNA clone, C2.3, whose transcripts were intensively expressed in RAG-1-inducible stromal cell line, but rarely expressed in RAG-1-non inducible mutant cell line (PA6). The cDNA sequence had no homology to the known genes. The sequence revealed an open reading frame that encodes a 221 amino acid protein with 4 potential transmembrane domains, suggesting a possible role of C2.3 product as a membrane receptor. Introduction of C2.3 cDNA into PA6 mutant line restored the ability to activate RAG-1 gene in lymphoid progenitors, indicating that a C2.3 product may be involved in the induction of RAG-1 gene activation.
Tagoh
H
H
Department of Immunology, Toyama Medical and Pharmaceutical University, Japan.
Kishi
H
H
Muraguchi
A
A
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D018076:DNA, Complementary; D005786:Gene Expression Regulation / Q000502:physiology; D006412:Hematopoietic Stem Cells / Q000378:metabolism*; D018398:Homeodomain Proteins; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics* / Q000502:physiology; D051379:Mice; D008969:Molecular Sequence Data; D009004:Monosaccharide Transport Proteins; D011506:Proteins / Q000235:genetics*; D015533:Transcriptional Activation
D018076:DNA, Complementary; D018398:Homeodomain Proteins; D008565:Membrane Proteins; D009004:Monosaccharide Transport Proteins; D011506:Proteins; C099350:SLC50A1 protein, human; C064658:RAG-1 protein