title: Primary structure of a cysteine proteinase inhibitor from the fruit of avocado (Persea americana Mill).
abstract: The complete amino acid sequence of a proteinaceous cysteine proteinase inhibitor from the fruit of avocado (avocado cystatin) is presented. The protein consists of 100 amino acid residues and has a molecular mass of 11,300 Da. Comparison of this sequence with sequences of plant cysteine proteinase inhibitors (phytocystatins), including oryzacystatins I and II from rice seeds, cowpea cystatin, and corn cystatin, showed that the avocado cystatin molecule has 60% and 54% residues identical with the two forms of the rice seed proteins, oryzacystatins I and II, respectively, and 64% and 63% with the cowpea and corn proteins, respectively. The totally conserved sequence, Gln-Val-Val-Ala-Gly, among several of the animal cystatins as well as phytocystatins, is at positions 47-51 in the avocado cystatin molecule.
authors.affiliation: Laboratory of Biochemistry, Faculty of Agriculture, Kyushu University, Fukuoka, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Kimura
authors.forename: T
authors.initials: T
authors.lastname: Ikeda
authors.forename: D
authors.initials: D
authors.lastname: Fukumoto
authors.forename: N
authors.initials: N
authors.lastname: Yamasaki
authors.forename: M
authors.initials: M
authors.lastname: Yonekura
mesh_terms: D000595:Amino Acid Sequence; D015891:Cystatins / Q000032:analysis*; D015853:Cysteine Proteinase Inhibitors / Q000032:analysis*; D005638:Fruit / Q000737:chemistry*; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000032:analysis*; D017386:Sequence Homology, Amino Acid
chemical_list: D015891:Cystatins; D015853:Cysteine Proteinase Inhibitors; D010940:Plant Proteins