title: Fluorescence in situ hybridization on human metaphase chromosomes detected by near-field scanning optical microscopy.
abstract: Fluorescence in situ hybridization on human metaphase chromosomes is detected by near-field scanning optical microscopy. This combination of cytochemical and scanning probe techniques enables the localization and identification of several fluorescently labelled genomic DNA fragments on a single chromosome with an unprecedented resolution. Three nucleic acid probes are used: pUC1.77, p1-79 and the plasmid probe alpha-spectrin. The hybridization signals are very well resolved in the near-field fluorescence images, while the exact location of the probes can be correlated accurately with the chromosome topography as afforded by the shear force image.
authors.affiliation: Department of Applied Physics, University of Twente, AE Enschede, The Netherlands.
authors.forename: M H
authors.initials: MH
authors.lastname: Moers
authors.forename: W H
authors.initials: WH
authors.lastname: Kalle
authors.forename: A G
authors.initials: AG
authors.lastname: Ruiter
authors.forename: J C
authors.initials: JC
authors.lastname: Wiegant
authors.forename: A K
authors.initials: AK
authors.lastname: Raap
authors.forename: J
authors.initials: J
authors.lastname: Greve
authors.forename: B G
authors.initials: BG
authors.lastname: de Grooth
authors.forename: N F
authors.initials: NF
authors.lastname: van Hulst
mesh_terms: D001710:Biotin; D002503:Centromere / Q000648:ultrastructure; D002875:Chromosomes / Q000737:chemistry* / Q000648:ultrastructure; D004247:DNA / Q000032:analysis* / Q000235:genetics; D015342:DNA Probes; D004076:Digoxigenin; D016650:Fluorescein-5-isothiocyanate; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008214:Lymphocytes; D008297:Male; D008677:Metaphase; D008856:Microscopy, Fluorescence; D013049:Spectrin / Q000235:genetics; D016615:Telomere / Q000648:ultrastructure
chemical_list: D015342:DNA Probes; D013049:Spectrin; D001710:Biotin; D004247:DNA; D016650:Fluorescein-5-isothiocyanate; D004076:Digoxigenin