Determination of loratadine and pheniramine from human serum by gas chromatography-mass spectrometry.
In this work, a method for the determination of the antihistaminic drugs loratadine and pheniramine from human serum is presented. Serum samples are extracted under basic conditions with hexane-n-amyl alcohol (95:5, v/v), the analytes are reextracted into diluted hydrochloric acid and, after basification, are once again extracted into the organic phase. The samples are measured by GC-MS. The limits o detection of the assay are 0.5 ng/ml for loratadine and 2 ng/ml for pheniramine. The R.S.D.s in the day-to-day precision test for loratadine are 7.0% at 20 ng/ml and 12.4% at 2 ng/ml. for pheniramine, the R.S.D. are 6.4% at 300 ng/ml and 10.2% at 20 ng/ml.
Martens
J
J
Department of Clinical Pharmacology, University Hospital, Magdeburg, Germany.
D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods* / Q000706:statistics & numerical data; D006634:Histamine H1 Antagonists / Q000097:blood*; D006801:Humans; D007700:Kinetics; D017336:Loratadine / Q000097:blood* / Q000493:pharmacokinetics; D008832:Microchemistry; D010632:Pheniramine / Q000097:blood* / Q000493:pharmacokinetics; D012680:Sensitivity and Specificity
D006634:Histamine H1 Antagonists; D010632:Pheniramine; D017336:Loratadine