title: Cloning of SEZ-12 encoding seizure-related and membrane-bound adhesion protein.
abstract: SEZ-12 is one of the seizure-related cDNAs which was isolated by differential hybridization from primary cultured neurons from the mouse cerebral cortex with or without pentylenetetrazol (PTZ). SEZ-12 expression is transiently down-regulated in the mouse brain by injection of PTZ. To characterize SEZ-12, isolation of full-length cDNA and nucleotide sequence analysis were performed. The deduced amino acid sequence of SEZ-12 revealed that it encodes membrane-bound C-type lectin and has a significant homology to that of human cDNA, DGCR2 and IDD, which were cloned from a balanced translocation breakpoint associated with the DiGeorge syndrome. The isolated cDNA was about 4 kb in length and the message was expressed ubiquitously in various organs with low-abundance. Previously, we also cloned a transmembrane protein which is probably involved in cell-cell interaction by the differential hybridization technique. These findings suggest that transmembrane signaling in neuronal cells may have an important role in PTZ-induced seizure.
authors.affiliation: Department of Molecular and Developmental Science, Tokai University, Kanagawa, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Kajiwara
authors.forename: H
authors.initials: H
authors.lastname: Nagasawa
authors.forename: K
authors.initials: K
authors.lastname: Shimizu-Nishikawa
authors.forename: T
authors.initials: T
authors.lastname: Ookura
authors.forename: M
authors.initials: M
authors.lastname: Kimura
authors.forename: E
authors.initials: E
authors.lastname: Sugaya
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015815:Cell Adhesion Molecules / Q000235:genetics*; D003001:Cloning, Molecular; D003292:Convulsants / Q000494:pharmacology; D018076:DNA, Complementary / Q000235:genetics; D006801:Humans; D037102:Lectins / Q000235:genetics*; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D051379:Mice; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics*; D010433:Pentylenetetrazole / Q000494:pharmacology; D019038:Platelet Glycoprotein GPIb-IX Complex; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
chemical_list: D015815:Cell Adhesion Molecules; D003292:Convulsants; D018076:DNA, Complementary; D037102:Lectins; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D019038:Platelet Glycoprotein GPIb-IX Complex; C094990:adhesion receptor; D010433:Pentylenetetrazole