Myometrial arginase activity increases with advancing pregnancy in the guinea pig.
OBJECTIVE
Arginase has been suggested to play an important role in cellular growth and development, particularly important to the fetus, by supplying L-ornithine for the synthesis of polyamines. The purpose of this investigation was to determine whether pregnancy alters myometrial arginase activity and whether estradiol was responsible for the change.


METHODS
Myometrium and kidney were obtained from nonpregnant and pregnant guinea pigs of known gestational age. Arginase activity was measured under physiologic conditions by the conversion carbon 14-labeled guanidino-L-arginine to carbon 14-labeled urea. The concentrations of the enzyme's substrate, L-arginine, and its principal metabolite, L-ornithine, were measured in myometrium from near-term pregnant animals by use of an amino acid analyzer. Finally, a group of random cycle guinea pigs received 500 microgram/kg estradiol for 5 days before the myometrium was removed.


RESULTS
Myometrial arginase activity in pregnant animals was more than double that of myometrium from nonpregnant animals by the time the first measurement was made at 0.14 gestation. It continued to rise, peaking at values >25-fold higher than the nonpregnant activity by 0.90 gestation. Arginase activity in the myometrium underlying the placental implantation site was >25 fold higher (p<0.05) than myometrium from nonpregnant animals when first studied at 0.63 gestation and 10-fold higher than the contralateral fundal myometrium at the same time of gestation. Myometrial arginase activity in the sterile horn of six pregnant animals was half that of the horn containing one or more pups, but still five times higher than that of nonpregnant animals. Renal arginase activity also rose with advancing pregnancy, but the magnitude of the increase (up to 2-fold) was much smaller than that observed in either the fundal or placental implantation site myometrium. Estradiol had no significant effect on myometrial arginase activity.


CONCLUSIONS
These studies demonstrate that pregnancy increases myometrial arginase activity and that the presence of placenta or fetus is necessary for the maximal effect.
Weiner
C P
CP
Wellcome Research Laboratories, Beckenham, United Kingdom.
Knowles
R G
RG
Stegink
L D
LD
Dawson
J
J
Moncada
S
S
D000818:Animals; D001119:Arginase / Q000378:metabolism*; D001120:Arginine / Q000378:metabolism; D010064:Embryo Implantation; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D006168:Guinea Pigs; D007668:Kidney / Q000201:enzymology; D009215:Myometrium / Q000187:drug effects / Q000201:enzymology*; D009952:Ornithine / Q000378:metabolism; D010920:Placenta / Q000201:enzymology; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000378:metabolism*; D013997:Time Factors; D014599:Uterus / Q000378:metabolism
D004958:Estradiol; D001120:Arginine; D009952:Ornithine; D001119:Arginase