Acidic phospholipids inhibit the phospholipase D activity of rat brain neuronal nuclei.
An oleate dependent form of phospholipase D is present in rat brain neuronal nuclei and both the hydrolytic and transphosphatidylation activities measured. Several acidic phospholipids were found to inhibit this activity in a dose dependent manner. The IC50 values varied from 3.5 microM for PIP2 to 200 microM for phosphatidic acid. The hydrolysis of PIP2 by phospholipase C would be expected to result in the disinhibition of the oleate dependent phospholipase D activity.
Kanfer
J N
JN
Department of Biochemistry and Molecular Biology, University of Manitoba, Winnipeg, Canada.
McCartney
D G
DG
Singh
I N
IN
Freysz
L
L
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D002308:Cardiolipins / Q000494:pharmacology; D002467:Cell Nucleus / Q000201:enzymology; D020404:Glycerophospholipids; D006160:Guanosine Triphosphate / Q000494:pharmacology; D007527:Isoenzymes / Q000037:antagonists & inhibitors; D009474:Neurons / Q000187:drug effects* / Q000201:enzymology; D019301:Oleic Acid; D009829:Oleic Acids / Q000494:pharmacology; D010712:Phosphatidic Acids / Q000378:metabolism / Q000494:pharmacology; D010715:Phosphatidylglycerols / Q000494:pharmacology; D019269:Phosphatidylinositol 4,5-Diphosphate; D018129:Phosphatidylinositol Phosphates / Q000494:pharmacology; D010718:Phosphatidylserines / Q000494:pharmacology; D010739:Phospholipase D / Q000037:antagonists & inhibitors*; D010743:Phospholipids / Q000494:pharmacology*; D051381:Rats; D010738:Type C Phospholipases / Q000378:metabolism
D002308:Cardiolipins; D020404:Glycerophospholipids; D007527:Isoenzymes; D009829:Oleic Acids; D010712:Phosphatidic Acids; D010715:Phosphatidylglycerols; D019269:Phosphatidylinositol 4,5-Diphosphate; D018129:Phosphatidylinositol Phosphates; D010718:Phosphatidylserines; D010743:Phospholipids; C051521:phosphatidylethanol; D019301:Oleic Acid; D006160:Guanosine Triphosphate; D000255:Adenosine Triphosphate; D010738:Type C Phospholipases; D010739:Phospholipase D