Dynamics of the GroEL-protein complex: effects of nucleotides and folding mutants.
Chaperonins are a ubiquitous class of ring-shaped oligomeric protein complexes that are of crucial importance for protein folding in vivo. Analysis of the underlying functional principles had relied mainly on model proteins the (un)folding of which is dominated by irreversible side-reactions. We used maltose-binding protein (MBP) as a substrate protein for GroEL, since the refolding of this protein is completely reversible and thus allows a detailed analysis of the molecular parameters that determine the interaction of GroEL with non-native protein. We show that MBP folding intermediates are effectively trapped by GroEL in a diffusion-controlled reaction. This complex is stabilized via unspecific hydrophobic interactions. Stabilization energies for wild-type MBP increasing linearly with ionic strength from 50 kJ/mol to 60 kJ/mol. Depending on the intrinsic folding rate and the hydrophobicity of the substrate protein, the interaction of GroEL with MBP folding intermediates leads to a dramatically decreased apparent refolding rate of MBP (wild-type) or a complete suppression of folding (MBP folding mutant Y283D). On the basis of our data, a quantitative kinetic model of the GroEL-mediated folding cycle is proposed, which allows simulation of the partial reactions of the binding and release cycles under all conditions tested. In the presence of ATP and non-hydrolysable analogues, MBP is effectively released from GroEL, since the overall dissociation constant is reduced by three orders of magnitude. Interestingly, binding of nucleotide does not change the off rate by more than a factor of 3. However the on-rate is decreased by at least two orders of magnitude. Therefore, the rebinding reaction is prevented and folding occurs in solution.
Sparrer
H
H
Institut für Biophysik & Physikalische Biochemie, Universität Regensburg, Germany.
Lilie
H
H
Buchner
J
J
D000227:Adenine Nucleotides / Q000378:metabolism*; D001667:Binding, Competitive; D002352:Carrier Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D018834:Chaperonin 60 / Q000378:metabolism*; D029968:Escherichia coli Proteins; D007700:Kinetics; D058619:Maltose-Binding Proteins; D009154:Mutation; D033902:Periplasmic Binding Proteins; D011485:Protein Binding; D017510:Protein Folding; D011498:Protein Precursors / Q000235:genetics / Q000378:metabolism
D000227:Adenine Nucleotides; D002352:Carrier Proteins; D018834:Chaperonin 60; D029968:Escherichia coli Proteins; D058619:Maltose-Binding Proteins; D033902:Periplasmic Binding Proteins; D011498:Protein Precursors; C028003:RbsB protein, E coli