title: The inflammatory response promotes cutaneous metastasis in hamsters infected with Leishmania (Viannia) panamensis.
abstract: The influence of nonspecific and immunologically elicited inflammatory responses on the development of metastatic lesions was examined in the hamster model of Leishmania (Viannia) panamensis infection. Delayed type hypersensitivity (DTH) responses were induced using the contact sensitizing agent DNFB (2, 4-dinitro-1-fluorobenzene) and infection with L. panamensis followed by intradermal application of leishmanin. Nonspecific inflammatory response was achieved by the surgical excision of toes. The inductive and eliciting procedures were performed on the ears and fore and hind paws of the right side of experimental groups of hamsters that were inoculated in the snout with a highly metastatic strain of L. panamensis (MHOM/COL/84/1099). Skin metastases were detected by physical evaluation at 15-day intervals over a period of 7-8 mo. Suspected metastases were parasitologically confirmed by culture of tissue fluid aspirated from the lesion. The frequency of metastatic lesions was greater in hamsters subjected to inflammatory stimuli (14/38) than control animals (6/33; P = 0.035). Likewise, the frequency of metastases at the site of induction and elicitation of inflammation (18/22 lesions) in the experimental groups was greater than that observed at the same site in control animals (5/11 lesions; P = 0.017). These findings support a causal relationship between inflammatory response and the development of lesions in this model of secondary disease caused by L. panamensis.
authors.affiliation: Centro Internacional de Entrenamiento e Investigaciones Médicas, Cali, Colombia.
authors.forename: B L
authors.initials: BL
authors.lastname: Travi
authors.forename: Y
authors.initials: Y
authors.lastname: Osorio
authors.forename: N G
authors.initials: NG
authors.lastname: Saravia
mesh_terms: D000818:Animals; D000953:Antigens, Protozoan / Q000276:immunology; D006224:Cricetinae; D004139:Dinitrofluorobenzene; D004195:Disease Models, Animal; D005260:Female; D006968:Hypersensitivity, Delayed; D007249:Inflammation; D007428:Intradermal Tests; D018334:Leishmania guyanensis / Q000276:immunology / Q000502:physiology*; D007897:Leishmaniasis, Mucocutaneous / Q000276:immunology / Q000473:pathology*; D008297:Male; D008647:Mesocricetus; D012867:Skin / Q000276:immunology / Q000293:injuries / Q000473:pathology*; D014947:Wounds and Injuries / Q000150:complications
chemical_list: D000953:Antigens, Protozoan; C032127:leishmanin; D004139:Dinitrofluorobenzene