Effects of nine active ingredients in Chinese herbal medicine sho-saiko-to on 2-(2-furyl)-3-(5-nitro-2-furyl)acrylamide mutagenicity.
The antimutagenic effects of nine active compounds in the Chinese herbal medicine "sho-saiko-to" on mutagenesis induced by a direct-acting mutagen, 2-(2-furyl)-3-(5-nitro-2-furyl)acrylamide (AF-2) were investigated in Salmonella typhimurium, strain TA100. The active compounds examined were classified into two major groups, saponins and flavonoids, the former comprising glycyrrhizin, saikosaponins a, c, and d, and ginsenosides Rb1 and Rg1, and the latter, baicalin, baicalein and wogonin. Saikosaponin a and ginsenoside Rb1 were found to reduce the mutagenicity of AF-2 significantly when applied post-AF-2-treatment in the Salmonella mutagenicity assay. Ginsenoside Rb1 also decreased the mutagenic activity of AF-2 in a simultaneous treatment protocol. The results indicate that saikosaponin a and ginsenoside Rb1 may enhance DNA repair, and ginsenoside Rb1 may also have the ability to inactivate the mutagenic activity of AF-2 directly. On the other hand, saikosaponin d and baicalin showed a slight enhancing effect. None of the compounds, except baicalein, showed any toxic effect on the test strain. These findings may be useful for the development of chemopreventive agents.
Ohtsuka
M
M
First Department of Pathology, Kurume University School of Medicine, Hita, Oita.
Fukuda
K
K
Yano
H
H
Kojiro
M
M
D000818:Animals; D016587:Antimutagenic Agents / Q000494:pharmacology*; D001711:Biotransformation; D004260:DNA Repair / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D044950:Flavanones; D005419:Flavonoids / Q000494:pharmacology* / Q000633:toxicity; D005668:Furylfuramide / Q000633:toxicity*; D036145:Ginsenosides; D008297:Male; D015394:Molecular Structure; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity*; D009828:Oleanolic Acid / Q000031:analogs & derivatives*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012486:Salmonella typhimurium / Q000187:drug effects* / Q000235:genetics; D012502:Sapogenins / Q000494:pharmacology; D012503:Saponins / Q000494:pharmacology*
D016587:Antimutagenic Agents; D004365:Drugs, Chinese Herbal; D044950:Flavanones; D005419:Flavonoids; D036145:Ginsenosides; D009153:Mutagens; D012502:Sapogenins; D012503:Saponins; D005668:Furylfuramide; C006680:baicalein; C018142:saiko-keishi-to; D009828:Oleanolic Acid; C025759:saikosaponin D