5'-regulatory region of Agrobacterium tumefaciens T-DNA gene 6b directs organ-specific, wound-inducible and auxin-inducible expression in transgenic tobacco.
The regulatory activity of a 826 bp DNA fragment located upstream of the pTiBo542 TL-DNA gene 6b coding region was analyzed in transgenic tobacco, using beta-glucuronidase (gus) as a reporter gene. The region was shown to drive organ-specific, wound- and auxin-inducible expression of the reporter, the effect being dependent on the type and concentration of auxin.
Bagyan
I L
IL
Centre of Bioengineering, Academy of Sciences of Russia, Moscow, Russia.
Revenkova
E V
EV
Pozmogova
G E
GE
Kraev
A S
AS
Skryabin
K G
KG
D016960:Agrobacterium tumefaciens / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D016384:Consensus Sequence; D004269:DNA, Bacterial / Q000235:genetics*; D004277:DNA, Single-Stranded / Q000235:genetics; D015870:Gene Expression / Q000187:drug effects*; D005798:Genes, Bacterial; D005966:Glucuronidase / Q000096:biosynthesis; D007210:Indoleacetic Acids / Q000494:pharmacology*; D008969:Molecular Sequence Data; D009928:Organ Specificity; D030821:Plants, Genetically Modified; D010947:Plants, Toxic; D011994:Recombinant Proteins / Q000096:biosynthesis; D012689:Sequence Homology, Nucleic Acid; D016385:TATA Box; D014026:Nicotiana / Q000378:metabolism*; D014947:Wounds and Injuries
D004269:DNA, Bacterial; D004277:DNA, Single-Stranded; D007210:Indoleacetic Acids; D011994:Recombinant Proteins; C036483:T-DNA; D005966:Glucuronidase