A polymorphic and hypervariable locus in the pseudoautosomal region of the CBA/H mouse sex chromosomes.
We have identified a genomic locus (DXYH1) that is polymorphic and hypervariable within the CBA/H colony. Using a panel of C57BL/6 x Mus spretus backcross offspring, it was mapped to the distal end of the X chromosome. Pseudoautosomal inheritance was demonstrated through three generations of CBA/H x CBA/H and CBA/H x C57BL/6 crosses and confirmed through linkage to the Sxr locus in X/Y Sxr x 3H1 crosses. Meiotic recombination frequencies place DXYH1 similar 28% into the pseudoautosomal region from the boundary. The de novo generation of CBA/H variant DXYH1 restriction fragment length polymorphisms during spermatogenesis is suggestive of the germline instability associated with hypermutable human minisatellites. The absence of DXYH1-related sequences in Mus spretus provides DNA sequence evidence to support the observed failure of X-Y pairing during meiosis and consequent hybrid infertility in C57BL/6 x Mus spretus male F1 offspring.
Fennelly
J
J
MRC Radiation and Genomic Stability Unit, Chilton, Oxon, OX11 ORD, United Kingdom.
Laval
S
S
Wright
E
E
Plumb
M
M
D000818:Animals; D005260:Female; D008040:Genetic Linkage; D005819:Genetic Markers; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA / Q000235:genetics*; D008811:Mice, Inbred DBA; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D012732:Sex Determination Analysis; D014960:X Chromosome; D014998:Y Chromosome
D005819:Genetic Markers