Mutagenic specificities and adduct distributions for 7-bromomethylbenz[a]anthracenes.
Mutation induction in the supF gene of the plasmid pS189 by 7-bromomethylbenz[a]anthracene and 7-bromomethyl-12-methylbenz[a]anthracene was examined. The former compound was substantially more mutagenic than the latter but a much greater proportion of the total mutations were located at mutation hotspots for the 12-methyl derivative. The overall correlation between sites of mutation and sites of polymerase arrest (an indicator of adduct formation) through the supF gene was poor. Although these bromocompounds should form only a single guanine adduct (unlike dihydrodiol epoxides that form both cis and trans adducts) more than one mutational change was found at a given site, although the predominant base substitution was G-->T for either compound.
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Chemistry of Carcinogenesis Laboratory, ABL-Basic Research Program, NCI-Frederick Cancer Research and Development Center, Frederick, MD 21702, USA.
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D001483:Base Sequence; D001551:Benz(a)Anthracenes / Q000378:metabolism / Q000633:toxicity*; D002461:Cell Line, Transformed; D005814:Genes, Viral / Q000187:drug effects* / Q000235:genetics; D005822:Genetic Vectors; D006801:Humans; D006842:Hydrocarbons, Brominated / Q000633:toxicity; D008969:Molecular Sequence Data; D009152:Mutagenicity Tests; D017354:Point Mutation; D015678:Viral Structural Proteins / Q000235:genetics*
D001551:Benz(a)Anthracenes; D006842:Hydrocarbons, Brominated; D015678:Viral Structural Proteins; C001903:7-bromomethylbenzanthracene