Messenger RNA expression of somatostatin receptor subtypes in human and rat gastric mucosae.
In several tissues including gastric mucosa, somatostatin displays various biological effects. Five seven-transmembrane-domain somatostatin receptor subtypes (SSTR1-5) have been recently cloned and only SSTR1 has been shown to be present in the human stomach. We used the polymerase chain reaction on reverse transcripts (RT-PCR) to characterize further the SSTR's mRNAs in human and rat gastric mucosae and in the human gastric tumoral cell-line HGTL. The SSTR1-5's mRNAs were found in both human fundic and antral mucosae as well as in the HGT1 cell and rat antrum. The four SSTR2-5's mRNA's but not SSTR1's were detected in the rat fundic mucosa. Furthermore, the use of rat isolated and purified fundic mucosal cells allowed us to localize SSTR2-5 in the parietal cell-enriched fraction, whereas SSTR2 and SSTR5 were the only subtypes found in the endocrine cell-enriched fraction. These results are the first to demonstrate the presence of five SSTR's mRNA subtypes in the stomach.
Le Romancer
M
M
Unité de Recherches Biologie et Pathogie de l'Epithelium Digestif, Paris, France.
Cherifi
Y
Y
Levasseur
S
S
Laigneau
J P
JP
Peranzi
G
G
Jais
P
P
Lewin
M J
MJ
Reyl-Desmars
F
F
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers; D005260:Female; D005748:Gastric Fundus; D005753:Gastric Mucosa / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D016376:Oligonucleotides, Antisense; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D017481:Receptors, Somatostatin / Q000096:biosynthesis*; D013274:Stomach Neoplasms; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
D017931:DNA Primers; D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger; D017481:Receptors, Somatostatin