Androgen-dependent expression of fibroblast growth factor-1 in submaxillary gland of mouse.
We have purified a 16,000 dalton protein that stimulates growth of human umbilical cord vein-derived endothelial cells (HUV-EC) from mouse submaxillary glands by using heparin-Sepharose affinity and C4 reverse phase chromatography. The purified molecule was identified as an FGF-1 on the basis of its biological activities, its affinity for heparin and its N-terminal amino-acid sequence. The concentrations of FGF-1 in the submaxillary gland of male or testosterone-treated female mice were about 12 times those of untreated females or castrated males. The 2.3 and 4.1 kb FGF-1 mRNAs were expressed in the glands of male mice older than 4 weeks but not in the glands of female mice. These results suggest that FGF-1 may have important functions for growth, differentiation and development of mouse submaxillary glands, and it may act as an endocrine hormone.
Okamoto
T
T
Department of Oral & Maxillofacial Surgery I, Hiroshima University School of Dentistry, Japan.
Myoken
Y
Y
Yabumoto
M
M
Osaki
T
T
Fujita
Y
Y
Whitney
R G
RG
Kan
M
M
Crabb
J W
JW
Sato
G H
GH
Kato
Y
Y
Takada
K
K
Sato
J D
JD
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002455:Cell Division; D002457:Cell Extracts; D002460:Cell Line; D004730:Endothelium, Vascular / Q000166:cytology; D005260:Female; D005346:Fibroblast Growth Factors / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012737:Sex Factors; D013363:Submandibular Gland / Q000378:metabolism*; D013739:Testosterone / Q000378:metabolism*
D002457:Cell Extracts; D012333:RNA, Messenger; D013739:Testosterone; D005346:Fibroblast Growth Factors