Simultaneous determination of serotonin, N-acetylserotonin and melatonin in the pineal gland of the juvenile golden hamster by high-performance liquid chromatography with electrochemical detection.
A simple and simultaneous determination of melatonin and its precursors, serotonin (5-HT) and N-acetylserotonin, was achieved by reversed-phase high-performance liquid chromatography with electrochemical detection. The addition of an ion-pairing agent, sodium 1-octanesulfonate, to the chromatographic mobile phase caused an increase of the retention time of 5-HT, and resulted in the successful simultaneous resolution of these three indoleamines. This method was used to quantitate these indoleamines in the pineal gland of juvenile golden hamsters.
Harumi
T
T
Department of Anatomy, Asahikawa Medical College, Japan.
Akutsu
H
H
Matsushima
S
S
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006224:Cricetinae; D004563:Electrochemistry; D008550:Melatonin / Q000032:analysis*; D008647:Mesocricetus; D010870:Pineal Gland / Q000737:chemistry*; D012701:Serotonin / Q000031:analogs & derivatives* / Q000032:analysis*
D012701:Serotonin; D008550:Melatonin; C006389:N-acetylserotonin