title: Transcriptional analysis of the mtA idiomorph of Neurospora crassa identifies two genes in addition to mtA-1.
abstract: In Neurospora crassa, mating and heterokaryon formation between opposite mating-types is controlled by a single locus with two alternate forms termed mt A and mt a. Previously, an open reading frame (mt A-1) that confers mating identity and heterokaryon incompatibility was characterized in the 5.3 kb mt A idiomorph. In this study, we describe the structural and transcriptional characterization of two additional genes in the mt A idiomorph, Mt A-2 and mt A-3. The 373 amino acid mt A-2 ORF has 23% identity to the SMR1 ORF of Podospora anserina. DNA sequence analysis of a mutation affecting ascospore to 129 amino acids. The 324 amino acids mt A-3 ORF has an HMG domain and shows 22% amino acid identity to SMR2 of P. anserina. Transcripts from mt A-2 and mt A-3 are constitutively expressed during both vegetative and sexual reproduction. The presence of upstream ORFs in the mt A-2 and mt A-3 transcripts suggests the possibility of post-transcriptional regulation of the expression mt A-2 and mt A-3 polypeptides.
authors.affiliation: Department of Botany, University of British Columbia, Vancouver, Canada.
authors.forename: A V
authors.initials: AV
authors.lastname: Ferreira
authors.forename: S
authors.initials: S
authors.lastname: Saupe
authors.forename: N L
authors.initials: NL
authors.lastname: Glass
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D016384:Consensus Sequence / Q000235:genetics; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics*; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics*; D015966:Gene Expression Regulation, Fungal / Q000235:genetics; D005800:Genes, Fungal / Q000235:genetics*; D008969:Molecular Sequence Data; D009492:Neurospora crassa / Q000737:chemistry / Q000235:genetics*; D016366:Open Reading Frames; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment; D017421:Sequence Analysis; D014158:Transcription, Genetic / Q000235:genetics
chemical_list: D004268:DNA-Binding Proteins; D005656:Fungal Proteins; C097997:MTA-1 protein, Neurospora crassa; C099382:MTA-2 protein, Neurospora crassa; C099383:MTA-3 protein, Neurospora crassa