NP-06: a novel anti-human immunodeficiency virus polypeptide produced by a Streptomyces species.
From an extract of a Streptomyces culture, we identified and purified a novel compound, NP-06, which is active against human immunodeficiency virus (HIV) in vitro. Analyses indicate that NP-06 is a hydrophobic 21-mer oligopeptide, N terminally cyclized through the side chain of Asp-9, containing two intramolecular cystine linkages with a molecular weight of 2,163.4. The 50% inhibitory concentrations were 2.8 and 1.3 microM when NP-06 was tested for in vitro anti-HIV-1 activity in ATH8 cells and phytohemagglutinin-activated peripheral blood mononuclear cells, respectively, NP-06 appears to block the early stage of HIV-1 infection, most likely at the stage of virus-cell fusion.
Chokekijchai
S
S
Medicine Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA.
Kojima
E
E
Anderson
S
S
Nomizu
M
M
Tanaka
M
M
Machida
M
M
Date
T
T
Toyota
K
K
Ishida
S
S
Watanabe
K
K
D000595:Amino Acid Sequence; D000998:Antiviral Agents / Q000737:chemistry / Q000494:pharmacology*; D006678:HIV / Q000187:drug effects*; D006801:Humans; D008969:Molecular Sequence Data; D010456:Peptides, Cyclic / Q000737:chemistry / Q000494:pharmacology*; D013302:Streptomyces / Q000378:metabolism*
D000998:Antiviral Agents; C097450:NP-06 cyclic peptide; D010456:Peptides, Cyclic; C085040:RP 71955