title: The response regulator SprE controls the stability of RpoS.
abstract: In Escherichia coli, the sigma factor, RpoS, is a central regulator in stationary-phase cells. We have identified a gene, sprE (stationary-phase regulator), as essential for the negative regulation of rpoS expression. SprE negatively regulates the rpoS gene product at the level of protein stability, perhaps in response to nutrient availability. The ability of SprE to destabilize RpoS is dependent on the ClpX/ClpP protease. Based on homology, SprE is a member of the response regulator family of proteins. SprE is the first response regulator identified that is implicated in the control of protein stability. Moreover, SprE is the first reported protein that appears to regulate rpoS in response to a specific environmental parameter.
authors.affiliation: Department of Molecular Biology, Princeton University, New Jersey 08544, USA.
authors.forename: L A
authors.initials: LA
authors.lastname: Pratt
authors.forename: T J
authors.initials: TJ
authors.lastname: Silhavy
mesh_terms: D000074183:ATPases Associated with Diverse Cellular Activities; D000251:Adenosine Triphosphatases / Q000378:metabolism; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics; D001426:Bacterial Proteins / Q000502:physiology*; D002452:Cell Count; D004268:DNA-Binding Proteins; D049071:Endopeptidase Clp; D004926:Escherichia coli / Q000235:genetics* / Q000254:growth & development; D029968:Escherichia coli Proteins; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D005947:Glucose / Q000378:metabolism; D018832:Molecular Chaperones; D011485:Protein Binding; D012697:Serine Endopeptidases / Q000378:metabolism; D012808:Sigma Factor / Q000235:genetics* / Q000378:metabolism; D014157:Transcription Factors; D014882:Water-Electrolyte Balance
chemical_list: D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; D018832:Molecular Chaperones; D012808:Sigma Factor; D014157:Transcription Factors; C098923:rssB protein, E coli; D012697:Serine Endopeptidases; D049071:Endopeptidase Clp; D000251:Adenosine Triphosphatases; C088072:ClpX protein, E coli; D000074183:ATPases Associated with Diverse Cellular Activities; D005947:Glucose