title: Purification and characterization of carbamoyl-phosphate synthetase from the deep-sea hyperthermophilic archaebacterium Pyrococcus abyssi.
abstract: Carbamoyl-phosphate synthetase was purified from the deep-sea hyperthermophilic archaebacterium Pyrococcus abyssi. This enzyme appears to be monomeric and uses ammonium salts as nitrogen donor. Its activity is inhibited by some nucleotides that compete with ATP. In contrast with the carbamoyl-phosphate synthetases investigated so far, this enzyme is very resistant to high temperature. Its low molecular mass (46.6 kDa) and its catalytic properties suggest that the gene coding for this enzyme is a previously postulated ancestor, whose duplication gave the genes coding for carbamoyl-phosphate synthetases and carbamate kinases.
authors.affiliation: Laboratoire de Biochimie des Signaux Régulateurs Cellulaires et Moléculaires, Université Pierre et Marie Curie, Paris, France.
authors.forename: C
authors.initials: C
authors.lastname: Purcarea
authors.forename: V
authors.initials: V
authors.lastname: Simon
authors.forename: D
authors.initials: D
authors.lastname: Prieur
authors.forename: G
authors.initials: G
authors.lastname: Hervé
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D001105:Archaea / Q000201:enzymology*; D002222:Carbamoyl-Phosphate Synthase (Ammonia) / Q000037:antagonists & inhibitors / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D002221:Carbamyl Phosphate / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D006874:Hydrostatic Pressure; D007526:Isoelectric Point; D009711:Nucleotides / Q000494:pharmacology; D017851:Phosphotransferases (Carboxyl Group Acceptor); D011487:Protein Conformation; D013696:Temperature
chemical_list: D004791:Enzyme Inhibitors; D009711:Nucleotides; D002221:Carbamyl Phosphate; D000255:Adenosine Triphosphate; D017851:Phosphotransferases (Carboxyl Group Acceptor); C019288:carbamate kinase; D002222:Carbamoyl-Phosphate Synthase (Ammonia)