title: Biochemical properties of cortical allograft bone using a new method of bone strength measurement. A comparison of fresh, fresh-frozen and irradiated bone.
abstract: There have been conflicting reports on the effects of gamma irradiation on the material properties of cortical allograft bone. To investigate changes which result from the method of preparation, test samples must be produced with similar mechanical properties to minimise variations other than those resulting from treatment. We describe a new method for the comparative measurement of bone strength using standard bone samples. We used 233 samples from six cadavers to study the effects of irradiation at a standard dose (28 kGy) alone and combined with deep freezing. We also investigated the effects of varying the dose from 6.8 to 60 kGy (n = 132). None of the treatments had any effect on the elastic behaviour of the samples, but there was a reduction in strength to 64% of control values (p < 0.01) after irradiation with 28 kGy. There was also a dose-dependent reduction in strength and in the ability of the samples to absorb work before failure. We suggest that irradiation may cause an alteration in the bone matrix of allograft bone, but provided it is used in situations in which loading is within its elastic region, then failure should not occur.
authors.affiliation: Northern General Hospital, Sheffield, England.
authors.forename: A J
authors.initials: AJ
authors.lastname: Hamer
authors.forename: J R
authors.initials: JR
authors.lastname: Strachan
authors.forename: M M
authors.initials: MM
authors.lastname: Black
authors.forename: C J
authors.initials: CJ
authors.lastname: Ibbotson
authors.forename: I
authors.initials: I
authors.lastname: Stockley
authors.forename: R A
authors.initials: RA
authors.lastname: Elson
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002102:Cadaver; D015925:Cryopreservation / Q000379:methods*; D004307:Dose-Response Relationship, Radiation; D004548:Elasticity; D005260:Female; D005269:Femur / Q000528:radiation effects* / Q000637:transplantation*; D005720:Gamma Rays / Q000009:adverse effects*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009926:Organ Preservation / Q000009:adverse effects* / Q000379:methods*; D013718:Tensile Strength; D014184:Transplantation, Homologous; D016474:Weight-Bearing