Molecular cloning of prohormone convertase 1 from the atrial gland of Aplysia.
We have screened an Aplysia atrial gland cDNA library using a prohormone convertase (PC)1 probe prepared by polymerase chain reaction (PCR) and have isolated an Aplysia PC1-related full-length 3.6-kb cDNA clone. The cDNA sequence (3,565 bp) encoded a putative preproendoprotease (APC1) of 703 amino acid residues that showed considerable sequence identity with other eukaryotic PC1s, and indicated a high degree of sequence identity with an Aplysia nervous system PC sequence (aPC1B). Northern blot analysis of atrial gland RNA identified two APC1 transcripts of 3.9 kb and 5.0 kb. APC1 is a candidate PC that may play an important role in the processing of egg-laying hormone (ELH)-related precursors in atrial gland secretory cells and represents one of the first examples of PC1 expression in an exocrine tissue.
Gorham
E L
EL
Department of Human Biological Chemistry and Genetics, The University of Texas Medical Branch, Galveston 77555, USA.
Nagle
G T
GT
Smith
J S
JS
Shen
H
H
Kurosky
A
A
D000818:Animals; D001048:Aplysia / Q000201:enzymology* / Q000235:genetics*; D016282:Aspartic Acid Endopeptidases / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D015342:DNA Probes; D018076:DNA, Complementary; D004702:Endocrine Glands / Q000201:enzymology; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009420:Nervous System / Q000201:enzymology; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D045664:Proprotein Convertase 1; D043484:Proprotein Convertases
D015342:DNA Probes; D018076:DNA, Complementary; D043484:Proprotein Convertases; C473656:Pcsk1 protein, mouse; D045664:Proprotein Convertase 1; D016282:Aspartic Acid Endopeptidases