Negative transcriptional regulation of PH081 expression in Saccharomyces cerevisiae.
The PH081 gene product functions as an inhibitor of the cyclin-cyclin-dependent kinase pair, Pho80-Pho85, and is required for derepression of acid phosphatase-encoding gene (PH05) expression. PH081 is the only known regulator of this system whose transcriptional expression is regulated by the level of inorganic phosphate. This effect is mediated by the gene products of the PH04 and PH02 (BAS2, GRF10) genes which act as transcription factors. Fine structural analysis of the PH081 promoter region has revealed the existence of a negative regulatory sequence (NRS). That is, removal of this element causes an approx. 4-fold increase in PH081 expression. The NRS functions in either orientation, but only when located downstream from activation sequences. Interestingly, this element shows significant homology to a sequence present in the promoter of the PH08 gene, encoding a phosphate-repressible alkaline phosphatase. An electrophoretic mobility shift assay (EMSA) using whole-cell extracts and a NRS-containing DNA fragment detects a protein which specifically binds to this element.
Creasy
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CL
Department of Microbiology and Immunology, Medical College of Pennsylvania, Philadelphia, 19102, USA.
Shao
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D
Begman
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LW
D000135:Acid Phosphatase / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D003001:Cloning, Molecular; D018844:Cyclin-Dependent Kinases / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal / Q000235:genetics*; D017930:Genes, Reporter / Q000235:genetics; D018398:Homeodomain Proteins; D007763:Lac Operon / Q000235:genetics; D026901:Membrane Transport Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D028061:Phosphate Transport Proteins; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012097:Repressor Proteins; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D017384:Sequence Deletion / Q000235:genetics; D015534:Trans-Activators / Q000235:genetics; D014157:Transcription Factors; D014158:Transcription, Genetic / Q000235:genetics
D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D018398:Homeodomain Proteins; D026901:Membrane Transport Proteins; C051611:PHO2 protein, S cerevisiae; C060292:PHO4 protein, S cerevisiae; C081227:PHO81 protein, S cerevisiae; D028061:Phosphate Transport Proteins; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; D015534:Trans-Activators; D014157:Transcription Factors; C055831:phosphate permease; D018844:Cyclin-Dependent Kinases; C058605:PHO85 protein, S cerevisiae; D000135:Acid Phosphatase