Construction, expression and characterisation of a single chain anti-tumour antibody (scFv)-IL-2 fusion protein.
We describe the production and preliminary characterisation of a fusion protein between interleukin-2 and a single-chain Fv version of the H17E2 anti-placental alkaline phosphatase (PLAP) antibody. This molecule could be used to target interleukin-2 to PLAP-expressing tumours.
Boleti
E
E
Department of Immunology, Royal Postgraduate Medical School, Hammersmith Hospital, London, UK.
Deonarain
M P
MP
Spooner
R A
RA
Smith
A J
AJ
Epenetos
A A
AA
George
A J
AJ
D000469:Alkaline Phosphatase / Q000378:metabolism*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism*; D000912:Antibodies, Neoplasm / Q000378:metabolism*; D001666:Binding Sites, Antibody; D002455:Cell Division; D002460:Cell Line; D003001:Cloning, Molecular; D004797:Enzyme-Linked Immunosorbent Assay; D007376:Interleukin-2 / Q000378:metabolism*; D007527:Isoenzymes / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D000912:Antibodies, Neoplasm; C106201:H17E2 monoclonal antibody; D007376:Interleukin-2; D007527:Isoenzymes; D011993:Recombinant Fusion Proteins; C055791:germ-cell AP isoenzyme; D000469:Alkaline Phosphatase