title: JAK2 is associated with the c-kit proto-oncogene product and is phosphorylated in response to stem cell factor.
abstract: Stem cell factor (SCF) is a hematopoietic growth factor that interacts with the receptor tyrosine kinase, c-kit. We have found that SCF-stimulates rapid and transient tyrosine phosphorylation of JAK2 in human and murine cell lines, as well as in normal human progenitor cells. JAK2 and c-kit were associated in unstimulated cells with further recruitment of JAK2 to the c-kit receptor complex after SCF stimulation. Treatment of cells with JAK2 antisense oligonucleotides resulted in a 46% decrease in SCF-induced proliferation. These data demonstrate that SCF induces tyrosine phosphorylation of JAK2 and suggest that JAK2 is a component of the SCF signal transduction pathway.
authors.affiliation: Division of Cancer Treatment, and Biological Carcinogenesis and Development Program, SAIC Frederick, National Cancer Institute, Frederick Cancer Research and Development Center, MD 21702, USA.
authors.forename: S R
authors.initials: SR
authors.lastname: Weiler
authors.forename: S
authors.initials: S
authors.lastname: Mou
authors.forename: C S
authors.initials: CS
authors.lastname: DeBerry
authors.forename: J R
authors.initials: JR
authors.lastname: Keller
authors.forename: F W
authors.initials: FW
authors.lastname: Ruscetti
authors.forename: D K
authors.initials: DK
authors.lastname: Ferris
authors.forename: D L
authors.initials: DL
authors.lastname: Longo
authors.forename: D
authors.initials: D
authors.lastname: Linnekin
mesh_terms: D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D006801:Humans; D053614:Janus Kinase 2; D051379:Mice; D008969:Molecular Sequence Data; D010766:Phosphorylation / Q000187:drug effects; D011505:Protein-Tyrosine Kinases / Q000378:metabolism*; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins; D019009:Proto-Oncogene Proteins c-kit / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects*; D019089:Stem Cell Factor / Q000494:pharmacology*
chemical_list: C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins; D019089:Stem Cell Factor; D011505:Protein-Tyrosine Kinases; D019009:Proto-Oncogene Proteins c-kit; C507924:JAK2 protein, human; C507926:Jak2 protein, mouse; D053614:Janus Kinase 2