Characterization of hitchhiker, a transposon insertion frequently associated with baculovirus FP mutants derived upon passage in the TN-368 cell line.
We characterize a new Lepidopteran transposon associated with FP mutations of baculoviruses. This transposon, designated hitchhiker, is 579 bp long with 39-bp imperfect inverted terminal repeats. hitchhiker inserts with extreme specificity for a single trinucleotide target site, TTA, within the 25K gene. This transposon is the most frequently identified insertion in serial passage baculovirus mutants isolated from TN-368 cells.
Bauser
C A
CA
Department of Biological Sciences, University of Notre Dame, Indiana 46556, USA. malcolm.j.fraser.1@en.edu
Elick
T A
TA
Fraser
M J
MJ
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D004251:DNA Transposable Elements / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics; D008969:Molecular Sequence Data; D017924:Nucleopolyhedroviruses / Q000235:genetics* / Q000378:metabolism; D012692:Serial Passage; D018411:Spodoptera / Q000166:cytology
D004251:DNA Transposable Elements; D004279:DNA, Viral