[Changes in the opsonizing capacity of the blood serum in rats after irradiation and after a combined radiation-thermal lesion].
We studied changes in the blood serum opsonic capacity after irradiation, thermal burn, or combined radiation-thermal injuries. It was shown using chemiluminescence of macrophages, that phagocytized zymosan opsonized by the serum of control or experimental animals, and inhibition of complement-dependent reactions of opsonization was observed only after combined radiation-thermal injuries and was rather short-term. Incubation of normal macrophages with a serum obtained after the studied influences decreased the chemiluminescent response of macrophages to latex absorption. The most pronounced decrease in the intensity of luminescence was observed in the presence of the serum of rats subjected to combined radiation-thermal injuries and was due, within 24 h, to the burn component and, within 3 and 7 days, to the summed burn and radiation components of combined radiation-thermal injuries. We propose that the decreased opsonic capacity of the serum is due to the appearance of inhibitors of phagocytosis in the blood of animals after combined radiation-thermal injuries, rather than to deficiency of opsonizing factors.
Khlopovskaia
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EI
Budagov
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RS
D000818:Animals; D002056:Burns / Q000276:immunology*; D008163:Luminescent Measurements; D017737:Macrophages, Peritoneal / Q000276:immunology / Q000528:radiation effects; D008297:Male; D009895:Opsonin Proteins / Q000097:blood / Q000528:radiation effects*; D010587:Phagocytosis / Q000528:radiation effects; D011833:Radiation Injuries, Experimental / Q000276:immunology*; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
D009895:Opsonin Proteins