title: Definition of a tumor suppressor gene locus on the short arm of chromosome 3 in squamous cell carcinoma of the head and neck by means of microsatellite markers.
abstract: BACKGROUND
  Tumor suppressor genes are important in the development of head and neck cancer. Using microsatellite markers that map close to the region 3p24-pter, we determined the frequency of allele loss close to this site with a view to narrowing the search for a putative tumor suppressor gene involved in the development of squamous cell carcinoma of the head and neck, which may facilitate future positional cloning techniques.
  
  
  METHODS
  Laboratory-based project with tumor and normal specimens subjected to molecular genetic analysis. Tumor-normal tissue DNA pairs were analyzed for allelic imbalance and microsatellite instability on chromosome 3p in the region 3p24-pter by the polymerase chain reaction and microsatellite markers D3S1304, D3S656, D3S1252, D3S1293, THRB, and D3S1266.
  
  
  METHODS
  Molecular genetics and oncology research laboratory.
  
  
  METHODS
  Paired tumor-normal DNA samples were obtained from 46 patients with tumors of the head and neck.
  
  
  METHODS
  Detection of loss of heterozygosity and microsatellite instability on chromosome 3 in the region 3p24-p25.1.
  
  
  RESULTS
  We found loss of heterozygosity with at least one marker in 48% of informative cases and loss of heterozygosity or microsatellite instability in 57% of informative cases. The minimal region of loss was found in the region bounded by D3S656 and D31293.
  
  
  CONCLUSIONS
  A putative tumor suppressor gene in head and neck cancer lies between D3S656 and D3S1293 in the 3p25.1 region.
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, University of Liverpool, England.
authors.forename: H
authors.initials: H
authors.lastname: Rowley
authors.forename: A
authors.initials: A
authors.lastname: Jones
authors.forename: D
authors.initials: D
authors.lastname: Spandidos
authors.forename: J
authors.initials: J
authors.lastname: Field
mesh_terms: D000483:Alleles; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology / Q000648:ultrastructure; D002893:Chromosomes, Human, Pair 3 / Q000235:genetics*; D004273:DNA, Neoplasm; D016147:Genes, Tumor Suppressor / Q000235:genetics*; D006580:Genetic Carrier Screening; D016678:Genome; D006258:Head and Neck Neoplasms / Q000235:genetics* / Q000473:pathology; D006801:Humans; D007012:Hypopharyngeal Neoplasms / Q000235:genetics* / Q000473:pathology; D007013:Hypopharynx / Q000473:pathology; D007621:Karyotyping; D007822:Laryngeal Neoplasms / Q000235:genetics / Q000473:pathology; D007830:Larynx / Q000473:pathology; D018895:Microsatellite Repeats; D009055:Mouth / Q000473:pathology; D009062:Mouth Neoplasms / Q000235:genetics / Q000473:pathology; D009959:Oropharyngeal Neoplasms / Q000235:genetics / Q000473:pathology; D009960:Oropharynx / Q000473:pathology; D016133:Polymerase Chain Reaction
chemical_list: D004273:DNA, Neoplasm