title: Normal human ovary and ovarian tumors express glycodelin, a glycoprotein with immunosuppressive and contraceptive properties.
abstract: Glycodelin is a glycoprotein with potent immunosuppressive and contraceptive activities. It reacts with antibodies against placental protein 14, or progesterone-associated endometrial protein, and has a unique carbohydrate structure. Previous nomenclature is misleading, because glycodelin is neither synthesized in the placenta nor is it endometrium specific. No ovarian synthesis of glycodelin has been demonstrated. We present evidence for glycodelin synthesis in the human ovary and ovarian tumors. In follicular phase, immunoperoxidase staining of microwave-treated tissue sections employing affinity-purified polyclonal antibodies localized glycodelin to areas of stromal cell condensation in ovarian cortex, theca interna, and the granulosa. In luteal phase, cortical stroma was negative or only weakly positive, whereas glycodelin was present in theca interna of the corpus luteum and luteinized granulosa cells and also in corpus albicans and Leydig cells of the ovarian hilus. In situ hybridization gave negative results for glycodelin mRNA in normal ovary, whereas in ovarian tumors strong expression of both the glycodelin mRNA and the protein were found in benign and malignant serous cystadenomas, mucinous ovarian tumors being negative. We conclude that glycodelin is synthesized in human ovarian tumors, and its occurrence in normal human ovary may represent either synthesis or a site of glycodelin action.
authors.affiliation: Department of Obstetrics and Gynecology, Helsinki University Central Hospital, University of Helsinki, Finland.
authors.forename: M
authors.initials: M
authors.lastname: Kämäräinen
authors.forename: I
authors.initials: I
authors.lastname: Leivo
authors.forename: R
authors.initials: R
authors.lastname: Koistinen
authors.forename: M
authors.initials: M
authors.lastname: Julkunen
authors.forename: U
authors.initials: U
authors.lastname: Karvonen
authors.forename: E M
authors.initials: EM
authors.lastname: Rutanen
authors.forename: M
authors.initials: M
authors.lastname: Seppälä
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015152:Blotting, Northern; D003271:Contraceptive Agents, Female / Q000494:pharmacology*; D003338:Corpus Luteum / Q000737:chemistry; D005260:Female; D000071816:Glycodelin; D006023:Glycoproteins / Q000032:analysis* / Q000235:genetics / Q000502:physiology*; D006801:Humans; D007150:Immunohistochemistry; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D017403:In Situ Hybridization; D008875:Middle Aged; D010051:Ovarian Neoplasms / Q000737:chemistry* / Q000378:metabolism / Q000473:pathology; D010053:Ovary / Q000737:chemistry* / Q000166:cytology / Q000378:metabolism; D011257:Pregnancy Proteins / Q000032:analysis* / Q000235:genetics / Q000502:physiology*; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism
chemical_list: D003271:Contraceptive Agents, Female; D000071816:Glycodelin; D006023:Glycoproteins; D007166:Immunosuppressive Agents; C045246:PAEP protein, human; D011257:Pregnancy Proteins; D012333:RNA, Messenger