A rat genetic map constructed by representational difference analysis markers with suitability for large-scale typing.
Representational difference analysis (RDA) was applied to isolate chromosomal markers in the rat. Four series of RDA [restriction enzymes, BamHI and HindIII; subtraction of ACI/N (ACI) amplicon from BUF/Nac (BUF) amplicon and vice versa] yielded 131 polymorphic markers; 125 of these markers were mapped to all chromosomes except for chromosome X. This was done by using a mapping panel of 105 ACI x BUF F2 rats. To complement the relative paucity of chromosomal markers in the rat, genetically directed RDA, which allows isolation of polymorphic markers in the specific chromosomal region, was performed. By changing the F2 driver-DNA allele frequency around the region, four markers were isolated from the D1Ncc1 locus. Twenty-five of 27 RDA markers were informative regarding the dot blot analysis of amplicons, hybridizing only with tester amplicons. Dot blot analysis at a high density per unit of area made it possible to process a large number of samples. Quantitative trait loci can now be mapped in the rat genome by processing a large number of samples with RDA markers and then by isolating markers close to the loci of interest by genetically directed RDA.
Toyota
M
M
Carcinogenesis Division, National Cancer Center Research Institute, Tokyo, Japan.
Canzian
F
F
Ushijima
T
T
Hosoya
Y
Y
Kuramoto
T
T
Serikawa
T
T
Imai
K
K
Sugimura
T
T
Nagao
M
M
D000483:Alleles; D000818:Animals; D015139:Blotting, Southern; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D004247:DNA / Q000302:isolation & purification; D015245:Deoxyribonuclease BamHI; D015247:Deoxyribonuclease HindIII; D004587:Electrophoresis, Agar Gel; D005819:Genetic Markers; D016133:Polymerase Chain Reaction; D051381:Rats / Q000145:classification / Q000235:genetics*; D011912:Rats, Inbred ACI / Q000235:genetics; D011915:Rats, Inbred BUF / Q000235:genetics; D015183:Restriction Mapping
D005819:Genetic Markers; D004247:DNA; D015245:Deoxyribonuclease BamHI; D015247:Deoxyribonuclease HindIII