Characteristics of phosphatidic acid-containing lipoproteins which selectively inhibit bitter taste: high affinity to frog tongue surface and hydrophobic model membranes.
In previous studies (Katsuragi and Kurihara (1993) Nature 365,213--214; Katsuragi et al. (1995) Pharm. Res. 12,658--662) we showed that a lipoprotein composed of phosphatidic acid (PA) and beta-lactoglobulin (LG) selectively suppressed the taste responses to bitter substances without affecting those to other taste stimuli in the frog and man, while complexes composed of other lipids except for phosphatidylserine and LG had little inhibitory activity. In the present study, we found that the lipoproteins having inhibitory activity are adsorbed on the frog tongue surface, while those having no inhibitory activity are not adsorbed. We also examined adsorption of the lipoproteins on model lipid membranes coated on a quartz-crystal microbalance by measuring changes in its frequency. The lipoproteins having inhibitory activity were well adsorbed on the hydrophobic lipid membranes, while the lipoproteins having no inhibitory activity were little adsorbed on the membranes. It seems that receptor sites for bitter substances on the taste cell membranes are hydrophobic and those for other taste stimuli such as salts, acids and sugars are hydrophilic. Hence, the binding of PA-LG to hydrophobic sites of the receptor membranes will lead to selective inhibition of bitterness.
Katsuragi
Y
Y
Kao Corporation, Food Products Research Laboratories, Kashima, Japan.
Sugiura
Y
Y
Otsuji
K
K
Kurihara
K
K
D000327:Adsorption; D000818:Animals; D001665:Binding Sites; D006801:Humans; D066298:In Vitro Techniques; D007782:Lactoglobulins / Q000737:chemistry / Q000494:pharmacology; D008074:Lipoproteins / Q000737:chemistry* / Q000494:pharmacology*; D008567:Membranes, Artificial; D010712:Phosphatidic Acids / Q000737:chemistry* / Q000494:pharmacology*; D011892:Rana catesbeiana; D013649:Taste / Q000187:drug effects* / Q000502:physiology*; D013650:Taste Buds / Q000187:drug effects / Q000502:physiology; D014059:Tongue / Q000187:drug effects* / Q000502:physiology*
D007782:Lactoglobulins; D008074:Lipoproteins; D008567:Membranes, Artificial; D010712:Phosphatidic Acids