Lymphocyte migration across the anterior and posterior blood-retinal barrier in vitro.
The migration of lymphocytes through monolayers of rat retinal pigment epithelium (RPE) and retinal vascular endothelium, which form the posterior and anterior blood-retinal barrier (BRB) respectively, was investigated in vitro. After a 4-hr assay the migration of untreated peripheral lymph node (PLN) cells through RPE monolayers was negligible (<1%) with only a small increase found after activation of the PLN cells with concanavalin A or by cross-linking CD3. Activation of the RPE with IFN-gamma augmented migration with maximal PLN cell migration being achieved with a combination of CD3 cross-linking and IFN-gamma activation (17% migration). The highest level of lymphocyte migration was observed with three CD4+ antigen-specific T cell lines specific for purified protein derivative (PPD; 33% migration), ovalbumin (OA; 31%), and S-antigen (S-Ag; 57%). Migration of both untreated and Con A-activated PLN cells through retinal endothelial cells (EC) from PVG rats was negligible, whereas the migration of the antigen-specific T cell lines was 23, 29 and 23% for PPD, OA, and S-Ag lines, respectively. Migration of these cell lines through retinal endothelium derived from Lewis rats was significantly greater (44% for PPD, 39% for OA, and 39% for S-Ag) which corresponded with a greater expression of ICAM-1 on the EC.
Devine
L
L
Department of Clinical Science, Institute of Ophthalmology, University College London, United Kingdom.
Lightman
S
S
Greenwood
J
J
D000818:Animals; D000941:Antigens / Q000276:immunology; D019393:Arrestin; D001813:Blood-Retinal Barrier; D002448:Cell Adhesion; D002460:Cell Line; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism; D005136:Eye Proteins / Q000276:immunology; D007371:Interferon-gamma / Q000494:pharmacology; D007375:Interleukin-1 / Q000494:pharmacology; D008198:Lymph Nodes / Q000166:cytology; D008213:Lymphocyte Activation; D016131:Lymphocyte Subsets / Q000187:drug effects / Q000502:physiology*; D016853:Muromonab-CD3 / Q000494:pharmacology; D010047:Ovalbumin / Q000276:immunology; D010857:Pigment Epithelium of Eye / Q000098:blood supply*; D051381:Rats; D011917:Rats, Inbred Lew; D011919:Rats, Inbred Strains; D017260:Receptor-CD3 Complex, Antigen, T-Cell / Q000276:immunology; D011994:Recombinant Proteins / Q000494:pharmacology; D012171:Retinal Vessels / Q000502:physiology*; D013047:Specific Pathogen-Free Organisms; D006377:T-Lymphocytes, Helper-Inducer / Q000187:drug effects / Q000276:immunology; D014373:Tuberculin / Q000276:immunology
D000941:Antigens; D019393:Arrestin; D005136:Eye Proteins; D007375:Interleukin-1; D016853:Muromonab-CD3; D017260:Receptor-CD3 Complex, Antigen, T-Cell; D011994:Recombinant Proteins; D014373:Tuberculin; D007371:Interferon-gamma; D010047:Ovalbumin