title: A v-SNARE implicated in intra-Golgi transport.
abstract: We report the identification of a putative v-SNARE (GOS-28), localized primarily to transport vesicles at the terminal rims of Golgi stacks. In vitro, GOS-28, A Golgi SNARE of 28 kD, is efficiently packaged into Golgi-derived vesicles, which are most likely COPI coated. Antibodies directed against GOS-28 block its ability to bind alpha-SNAP, partially inhibit transport from the cis to the medial cisternae, and do not inhibit budding of COP-coated vesicles, but do accumulate docked uncoated vesicles.
authors.affiliation: Cellular Biochemistry and Biophysics Program, Memorial Sloan-Kettering Cancer Center, New York 10021, USA.
authors.forename: M
authors.initials: M
authors.lastname: Nagahama
authors.forename: L
authors.initials: L
authors.lastname: Orci
authors.forename: M
authors.initials: M
authors.lastname: Ravazzola
authors.forename: M
authors.initials: M
authors.lastname: Amherdt
authors.forename: L
authors.initials: L
authors.lastname: Lacomis
authors.forename: P
authors.initials: P
authors.lastname: Tempst
authors.forename: J E
authors.initials: JE
authors.lastname: Rothman
authors.forename: T H
authors.initials: TH
authors.lastname: Söllner
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000918:Antibody Specificity; D001483:Base Sequence; D001667:Binding, Competitive / Q000502:physiology; D001692:Biological Transport / Q000502:physiology; D016466:CHO Cells / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure; D002352:Carrier Proteins / Q000276:immunology / Q000378:metabolism; D002458:Cell Fractionation; D018699:Coated Vesicles / Q000737:chemistry; D006224:Cricetinae; D003593:Cytoplasm / Q000737:chemistry; D006056:Golgi Apparatus / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D007425:Intracellular Membranes / Q000737:chemistry; D008565:Membrane Proteins / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D016253:Microscopy, Immunoelectron; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000302:isolation & purification / Q000378:metabolism; D050600:SNARE Proteins; D052067:Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins; D033921:Vesicular Transport Proteins
chemical_list: D002352:Carrier Proteins; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D050600:SNARE Proteins; D052067:Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins; D033921:Vesicular Transport Proteins