title: Determination of epitestosterone and testosterone in urine by high-performance liquid chromatography.
abstract: This paper describes an alternative HPLC method for the determination of testosterone and epitestosterone, which is simple, rapid, selective, sensitive and reproducible. Samples were prepared using a previous enzymatic hydrolysis with liquid-liquid extraction. The determination was carried out on a Hypersil BDS-C18 reversed-phase column with gradient elution and UV absorbance detection (240 nm). The limits of quantification (signal-to-noise ratio = 6) were 20 ng/ml for testosterone and 30 ng/ml for epitestosterone.
authors.affiliation: Laboratorio de Control de Dopaje, Consejo Superior de Deportes, Madrid, Spain.
authors.forename: R
authors.initials: R
authors.lastname: Navajas
authors.forename: C
authors.initials: C
authors.lastname: Imaz
authors.forename: D
authors.initials: D
authors.lastname: Carreras
authors.forename: M
authors.initials: M
authors.lastname: García
authors.forename: M
authors.initials: M
authors.lastname: Pérez
authors.forename: C
authors.initials: C
authors.lastname: Rodríguez
authors.forename: A F
authors.initials: AF
authors.lastname: Rodríguez
authors.forename: R
authors.initials: R
authors.lastname: Cortés
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000706:statistics & numerical data; D004845:Epitestosterone / Q000652:urine*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013739:Testosterone / Q000652:urine*
chemical_list: D013739:Testosterone; D004845:Epitestosterone