Gonadotropin hyperstimulation influences the 35S-methionine metabolism of mouse preimplantation embryos.
The effects of gonadotropin stimulation on mouse embryo uptake and incorporation of 35S-methionine were studied. We found that the uptake of 35S-methionine was reduced in embryos of stimulated females in both the two-cell and the blastocyst developmental stage. The incorporation of 35S-methionine into protein was not statistically significantly different between the embryos of stimulated and those of unstimulated females. Qualitatively, protein synthesis was equal in both groups as determined with one-dimensional SDS-PAGE. The results are discussed and we conclude that mouse embryo viability in vivo is decreased by ovarian stimulation.
Wetzels
A M
AM
Department of Gynecology and Obstetrics, University Hospital Nijmegen, The Netherlands.
Artz
M T
MT
Goverde
H J
HJ
Bastiaans
B A
BA
Hamilton
C J
CJ
Rolland
R
R
D000818:Animals; D001755:Blastocyst / Q000187:drug effects / Q000378:metabolism*; D005314:Embryonic and Fetal Development; D005260:Female; D006062:Gonadotropins / Q000494:pharmacology*; D008715:Methionine / Q000378:metabolism*; D051379:Mice; D014176:Protein Biosynthesis
D006062:Gonadotropins; D008715:Methionine