title: An improved technique for the cryopreservation of Gaigeria pachyscelis (Sandveld hookworm).
abstract: A technique for the cryopreservation of third-stage larvae of Gaigeria pachyscelis is described. It consists of incubating sheathed infective larvae at 37 degrees C in 40% (v/v) ethylene glycol for 7 min, followed by 2 min at 0 degree C in 82.8% (v/v) cryoprotectant mixture, prior to transferring the larvae to liquid nitrogen. The survival rate obtained with this technique is consistently high: 69.1% as assessed by motility.
authors.affiliation: Helminthology Division, Onderstepoort Veterinary Institute, South Africa.
authors.forename: G A
authors.initials: GA
authors.lastname: Titoy
mesh_terms: D000723:Ancylostomatoidea; D000818:Animals; D015925:Cryopreservation / Q000379:methods*; D007814:Larva; D013019:South Africa