Long-lasting immune response induced by recombinant bacillus Calmette-Guérin (BCG) secretion system.
The recombinant bacillus Calmette-Guérin (rBCG) secretion system utilizing an extracellular alpha antigen of Mycobacterium kansasii (alpha-K) was characterized biochemically and immunologically. The human immunodeficiency virus type 1 (HIV-1) p17gag B cell epitope fused to alpha-K was secreted in extremely large amounts. At least three mice out of seven inoculated with rBCG generated high titres of antibody to the epitope. The long-lasting antibody production persisted more than 14 months.
Wada
N
N
School of Dentistry, Nagasaki University, Nagasaki City, Japan.
Ohara
N
N
Kameoka
M
M
Nishino
Y
Y
Matsumoto
S
S
Nishiyama
T
T
Naito
M
M
Yukitake
H
H
Okada
Y
Y
Ikuta
K
K
Yamada
T
T
D000595:Amino Acid Sequence; D000818:Animals; D001500:BCG Vaccine / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D000939:Epitopes / Q000235:genetics / Q000276:immunology; D015683:Gene Products, gag / Q000235:genetics / Q000276:immunology; D005822:Genetic Vectors / Q000276:immunology* / Q000378:metabolism*; D015483:HIV Antibodies / Q000096:biosynthesis / Q000235:genetics; D015488:HIV Antigens / Q000235:genetics / Q000276:immunology; D015497:HIV-1 / Q000235:genetics / Q000276:immunology; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D011993:Recombinant Fusion Proteins / Q000378:metabolism*; D013997:Time Factors; D014614:Vaccines, Synthetic / Q000276:immunology* / Q000378:metabolism*; D014764:Viral Proteins; D054301:gag Gene Products, Human Immunodeficiency Virus
D001500:BCG Vaccine; D000939:Epitopes; D015683:Gene Products, gag; D015483:HIV Antibodies; D015488:HIV Antigens; D011993:Recombinant Fusion Proteins; D014614:Vaccines, Synthetic; D014764:Viral Proteins; D054301:gag Gene Products, Human Immunodeficiency Virus; C054858:p17 protein, Human Immunodeficiency Virus Type 1