title: In vitro progenitor analysis in a Diamond Blackfan anaemia patient who responded once but not twice to interleukin-3 therapy, using short-term and long-term cultures and c-kit analysis.
abstract: Interleukin-3 (IL-3) therapy as a treatment for Diamond Blackfan anaemia (DBA) patients has been largely disappointing despite early hope it would be suitable for stimulating arrested erythropoiesis. Initial hope came from in vitro discoveries that IL-3 (+EPO) generated well-haemoglobinized BFU-E colonies in some patients, but was soon tempered by the realization that in vitro and in vivo IL-3 response did not, in the majority of cases, correlate. Nevertheless in vitro testing has been the main focus in analysing the abnormality in the stem and progenitor cell compartment in DBA. Here we report in vitro analysis of a DBA patient who responded once to IL-3 therapy, but not a second time following relapse, using short-term culture, long-term culture and c-kit analysis. Progenitor numbers before and after the first therapy were in the high normal range, but after relapse were much reduced below normal levels. Long-term cultures suggested some arrested progenitors had been reactivated into normal cycle by the first therapy, but may not have been replaced by more immature progenitors. c-kit analysis revealed increased expression in all tested cell populations. These results imply that the first IL-3 therapy reactivated some erythroid progenitors but left the progenitor pool depleted when more immature cells remained arrested.
authors.affiliation: Division of Haematology, St George's Hospital Medical School, London, UK.
authors.forename: C P
authors.initials: CP
authors.lastname: McGuckin
authors.forename: M R
authors.initials: MR
authors.lastname: Uhr
authors.forename: S E
authors.initials: SE
authors.lastname: Ball
authors.forename: E C
authors.initials: EC
authors.lastname: Gordon-Smith
mesh_terms: D002452:Cell Count; D002478:Cells, Cultured; D002675:Child, Preschool; D003114:Colony-Forming Units Assay; D015672:Erythroid Precursor Cells / Q000473:pathology*; D004921:Erythropoietin / Q000494:pharmacology; D005199:Fanconi Anemia / Q000473:pathology* / Q000628:therapy; D005260:Female; D006801:Humans; D007377:Interleukin-3 / Q000627:therapeutic use*; D019009:Proto-Oncogene Proteins c-kit / Q000032:analysis*; D017211:Treatment Failure
chemical_list: D007377:Interleukin-3; D004921:Erythropoietin; D019009:Proto-Oncogene Proteins c-kit