title: Characterization of dp6troglycan-laminin interaction in peripheral nerve.
abstract: Dystoroglycan is encoded by a single gene and cleaved into two proteins, alpha and beta-dystroglycan, by posttranslational processing. The 120kDa peripheral nerve isoform of alpha-dystroglycan binds laminin-2 comprised of the alpha 2, beta 1, and gamma 1 chains. In congenital muscular dystrophy and dy mice deficient in laminin alpha 2 chain, peripheral myelination is disturbed, suggesting a role for the dystroglycan- laminin interaction in peripheral myelinogenesis. To begin to test this hypothesis, we have characterized the dystroglycan-laminin interaction in peripheral nerve. We demonstrate that (1) alpha-dystroglycan is an extracellular peripheral membrane glycoprotein that links beta-dystroglycan in the Schwann cell outer membrane with laminin-2 in the endoneurial basal lamina, and (2) dystrophin homologues Dp116 and utrophin are cytoskeletal proteins of the Schwann cell cytoplasm. We also present data that suggest a role for glycosylation of alpha-dystroglycan in the interaction with laminin.
authors.affiliation: Department of Neurology and Neuroscience, Teikyo University School of Medicine, Tokyo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Yamada
authors.forename: A
authors.initials: A
authors.lastname: Chiba
authors.forename: T
authors.initials: T
authors.lastname: Endo
authors.forename: A
authors.initials: A
authors.lastname: Kobata
authors.forename: L V
authors.initials: LV
authors.lastname: Anderson
authors.forename: H
authors.initials: H
authors.lastname: Hori
authors.forename: H
authors.initials: H
authors.lastname: Fukuta-Ohi
authors.forename: I
authors.initials: I
authors.lastname: Kanazawa
authors.forename: K P
authors.initials: KP
authors.lastname: Campbell
authors.forename: T
authors.initials: T
authors.lastname: Shimizu
authors.forename: K
authors.initials: K
authors.lastname: Matsumura
mesh_terms: D000818:Animals; D002241:Carbohydrates / Q000502:physiology; D002417:Cattle; D003598:Cytoskeletal Proteins / Q000378:metabolism* / Q000648:ultrastructure; D049030:Dystroglycans; D016189:Dystrophin / Q000378:metabolism* / Q000648:ultrastructure; D007797:Laminin / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000378:metabolism* / Q000648:ultrastructure; D010525:Peripheral Nerves / Q000378:metabolism*; D011817:Rabbits; D012584:Sciatic Nerve / Q000378:metabolism*
chemical_list: D002241:Carbohydrates; D003598:Cytoskeletal Proteins; D016189:Dystrophin; D007797:Laminin; D008562:Membrane Glycoproteins; D049030:Dystroglycans