Cloning and characterization of the bovine Fas.
Fas (APO-1, CD95), a member of the tumor necrosis factor (TNF) receptor superfamily, is a cell membrane protein that mediates programmed cell death (apoptosis). In an effort to characterize the possible role of Fas-mediated apoptosis in some important physiological processes in livestock, bovine Fas (bFas) cDNA was isolated and its nucleotide sequence determined. The predicted amino acid sequence encodes a 323-amino-acid protein that contains a leader peptide, a transmembrane domain, and three domains of cysteine-rich motif within the extracellular region. At the amino acid level, bFas is 57% and 50% identical to human (hFas) and mouse (mFas), respectively. Its expression is abundant in peripheral blood lymphocytes, lung, spleen, thymus, and ovary, but minimal in heart, liver, and brain. A polyclonal anti-bFas serum raised against the carboxy-terminal half of cysteine-rich motif I recognized a single 46-kD protein in bovine MDBK cells by Western blot analysis. To investigate the apoptotic activity of bFas, MDBK and bFas-transfected L929 cells were exposed to a monoclonal anti-hFas IgM. Unlike other cell culture systems, the antibody failed to trigger cell death in MDBK and bFas transfected L929 cells.
Yoo
J
J
U.S. Department of Agriculture, Agricultural Research Service, Roman L. Hruska U.S. Meat Animal Research Center (MARC), Clay Center, Nebraska 68933-0166, USA.
Stone
R T
RT
Beattie
C W
CW
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002460:Cell Line; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D015870:Gene Expression; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000276:immunology; D012333:RNA, Messenger / Q000235:genetics; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D012995:Solubility; D014018:Tissue Distribution; D019014:fas Receptor / Q000737:chemistry / Q000235:genetics*
D018076:DNA, Complementary; D010455:Peptides; D012333:RNA, Messenger; D019014:fas Receptor