Construction of mini-F plasmid vectors for plasmid shuffling in Escherichia coli.
We have constructed two Escherichia coli mini-F plasmid vectors, pJK286 and pJK289, which have unique EcoRI, BamHI and HindIII sites downstream from the lac promoter. The mini-F vectors are useful for plasmid shuffling, with which we can efficiently carry out localized mutagenesis.
Kato
J
J
Department of Molecular Biology, University of Tokyo, Japan.
Ikeda
H
H
D002876:Chromosomes, Bacterial / Q000235:genetics; D003001:Cloning, Molecular / Q000379:methods*; D004269:DNA, Bacterial / Q000235:genetics; D004274:DNA, Recombinant / Q000235:genetics; D015245:Deoxyribonuclease BamHI; D015246:Deoxyribonuclease EcoRI; D015247:Deoxyribonuclease HindIII; D004926:Escherichia coli / Q000235:genetics*; D005144:F Factor / Q000235:genetics*; D018390:Gene Targeting; D005822:Genetic Vectors / Q000235:genetics*; D007763:Lac Operon / Q000235:genetics; D011401:Promoter Regions, Genetic
D004269:DNA, Bacterial; D004274:DNA, Recombinant; D015245:Deoxyribonuclease BamHI; D015246:Deoxyribonuclease EcoRI; D015247:Deoxyribonuclease HindIII