Nitrite-induced export of glutathione disulfide from erythrocytes.
Export of glutathione disulfide (GSSG) from human erythrocytes induced by several agents (H2O2, t-butyl hydroperoxide, phenazine methosulfate and NaNO2/) was compared. NaNO2 treatment was found optimal for induction of GSSG transport. GSSG was exported from NaNO2-treated erythrocytes mainly via the low-affinity kinetic component. The transport rate increased with increasing pH in the pH range of 6.5-8.0. The transport was inhibited by fluoride, vanadate and intracellular DNP-SG. On the basis of the obtained results, a possibility of existence of one common low-affinity transporter for glutathione S-conjugates and GSSG is discussed.
Lapshina
E A
EA
Department of Molecular Biophysics, University of Lódź, Poland.
Bartosz
G
G
D001692:Biological Transport / Q000187:drug effects; D004912:Erythrocytes / Q000187:drug effects* / Q000378:metabolism; D005978:Glutathione / Q000031:analogs & derivatives* / Q000097:blood; D019803:Glutathione Disulfide; D006801:Humans; D012016:Reference Values; D012977:Sodium Nitrite / Q000494:pharmacology*
D005978:Glutathione; D012977:Sodium Nitrite; D019803:Glutathione Disulfide