Inducers of adipose conversion activate transcription promoted by a peroxisome proliferators response element in 3T3-L1 cells.
Nonmetabolizable fatty acids are shown here to induce adipose conversion of 3T3-L1 preadipocytes as well as to activate transcription of a reporter plasmid promoted by a peroxisome proliferators response element. This dual activity was also observed using the peroxisome proliferator bezafibrate or the differentiation inducer isobutyl methylxanthine. The data suggest a role for a peroxisome proliferators activated receptor (PPAR) in adipose conversion induced by fatty acids, isobutyl methylxanthine, or xenobiotic amphipathic carboxylates.
Brandes
R
R
Department of Human Nutrition and Metabolism, Hebrew University, Faculty of Medicine, Jerusalem, Israel.
Arad
R
R
Bar-Tana
J
J
D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D016475:3T3 Cells; D000273:Adipose Tissue / Q000187:drug effects*; D000818:Animals; D001629:Bezafibrate / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D015500:Chloramphenicol O-Acetyltransferase / Q000032:analysis / Q000235:genetics; D007004:Hypoglycemic Agents / Q000494:pharmacology; D000960:Hypolipidemic Agents / Q000494:pharmacology*; D051379:Mice; D008830:Microbodies / Q000187:drug effects*; D010168:Palmitates / Q000494:pharmacology; D010169:Palmitic Acids / Q000494:pharmacology; D010957:Plasmids; D018160:Receptors, Cytoplasmic and Nuclear / Q000187:drug effects* / Q000235:genetics; D014157:Transcription Factors / Q000187:drug effects* / Q000235:genetics; D014158:Transcription, Genetic / Q000187:drug effects; D014162:Transfection
D007004:Hypoglycemic Agents; D000960:Hypolipidemic Agents; D010168:Palmitates; D010169:Palmitic Acids; D018160:Receptors, Cytoplasmic and Nuclear; D014157:Transcription Factors; C022776:2-bromopalmitate; C056166:MEDICA 16; D015500:Chloramphenicol O-Acetyltransferase; D015056:1-Methyl-3-isobutylxanthine; D001629:Bezafibrate