title: Exploring the functional robustness of an enzyme by in vitro evolution.
abstract: The evolution of natural proteins is thought to have occurred by successive fixation of individual mutations. In vitro protein evolution seeks to accelerate this process. RNA hypermutagenesis, cDNA synthesis in the presence of biased dNTP concentrations, delivers elevated mutant and mutation frequencies. Here lineages of active enzymes descended from the homotetrameric 78 residue dihydrofolate reductase (DHFR) encoded by the Escherichia coli R67 plasmid were generated by iterative RNA hypermutagenesis, resulting in >20% amino acid replacement. The 22 residue N-terminus could be deleted yielding a minimum functional entity refractory to further changes, designating it as a determinant of R67 robustness. Complete substitution of the segment still allowed fixation of mutations. By the facile introduction of multiple mutations, RNA hypermutagenesis allows the generation of active proteins derived from extant genes through a mode unexplored by natural selection.
authors.affiliation: Unité de Rétrovirologie Moléculaire, Institut Pasteur, Paris, France.
authors.forename: M A
authors.initials: MA
authors.lastname: Martinez
authors.forename: V
authors.initials: V
authors.lastname: Pezo
authors.forename: P
authors.initials: P
authors.lastname: Marlière
authors.forename: S
authors.initials: S
authors.lastname: Wain-Hobson
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D005075:Biological Evolution; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D008969:Molecular Sequence Data; D016296:Mutagenesis; D017354:Point Mutation; D012329:RNA, Bacterial / Q000235:genetics; D017384:Sequence Deletion; D017386:Sequence Homology, Amino Acid; D013329:Structure-Activity Relationship; D013762:Tetrahydrofolate Dehydrogenase / Q000235:genetics* / Q000378:metabolism; D014296:Trimethoprim Resistance / Q000235:genetics
chemical_list: D012329:RNA, Bacterial; C098921:dihydrofolate reductase type II; D013762:Tetrahydrofolate Dehydrogenase