Cloning and sequencing of the 5' region of the human glucose-6-phosphatase gene: transcriptional regulation by cAMP, insulin and glucocorticoids in H4IIE hepatoma cells.
We have cloned and sequenced the first 1.2 kb of the 5' region of the human glucose-6-phosphatase gene. Transfection of H4IIE hepatoma cells with the 1.2 kb fragment fused to a luciferase reporter gene demonstrated both basal and hormone responsive luciferase activity. Dexamethasone increased and insulin decreased luciferase activity. Insulin and dibutyryl cyclic AMP both significantly decreased activity in the presence of dexamethasone.
Schmoll
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Department of Obstetrics and Gynaecology, Ninewells Hospital and Medical School, University of Dundee, UK.
Allan
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Burchell
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A
D000818:Animals; D001483:Base Sequence; D003994:Bucladesine / Q000494:pharmacology*; D003001:Cloning, Molecular; D003907:Dexamethasone / Q000494:pharmacology*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D017930:Genes, Reporter; D005952:Glucose-6-Phosphatase / Q000235:genetics*; D006801:Humans; D007328:Insulin / Q000494:pharmacology*; D008114:Liver Neoplasms, Experimental; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics; D051381:Rats; D014158:Transcription, Genetic; D014162:Transfection / Q000235:genetics; D014407:Tumor Cells, Cultured
D007328:Insulin; D003994:Bucladesine; D003907:Dexamethasone; D005952:Glucose-6-Phosphatase