title: T-cell infiltration of primary CNS lymphoma.
abstract: We stained 13 primary CNS lymphomas (PCNSLs) (six from patients with AIDS, seven from immunocompetent patients) with a panel of antibodies to T cells (pan T cell [CD3], T helper cell [CD4], T suppressor cell [CD8], delta/delta cell [CD4-8-]), B cells (CD20), hematopoietic cells (T200), and NK cell (CD56). We estimated the percentage of tumor cells staining with each antibody. All tumors were B-cell lymphomas. The non-AIDS tumors showed a significant infiltration with CD3+ cells (mean of 10.82% of total cells). The AIDS patients' tumors showed a smaller percentage of CD3+ infiltrating cells (mean, 4.88% of total cells) (p<0.01). CD4+ cells were 9.11% of the total hematopoietic cells in the non-AIDS patients and 3.13% in AIDS patients (p<0.01). AIDS patients showed some CD8+ cells (0.3%), which was significantly higher than in immunocompetent patients (0%) (p<0.05). Very few tumor cells stained with the NK cell and delta/delta cell markers. Both immunocompetent and AIDS patients with PCNSL exhibit significant CD3+ and CD4+ cell infiltration of their tumors; this infiltration is significantly lower in AIDS patients. AIDS patients show a minor CD8+ cell infiltration of their tumors. These results on PCNSL are different from systemic lymphomas, which show a higher CD4 and CD8 cell infiltration, and may offer insights into the more aggressive nature of AIDS-related PCNSL.
authors.affiliation: Department of Internal Medicine, University of Nebraska Medical Center, Omaha,NE 68198-2045, USA.
authors.forename: R
authors.initials: R
authors.lastname: Bashir
authors.forename: M
authors.initials: M
authors.lastname: Chamberlain
authors.forename: E
authors.initials: E
authors.lastname: Ruby
authors.forename: F H
authors.initials: FH
authors.lastname: Hochberg
mesh_terms: D015703:Antigens, CD / Q000032:analysis; D001932:Brain Neoplasms / Q000473:pathology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D002452:Cell Count; D016543:Central Nervous System Neoplasms / Q000473:pathology*; D006801:Humans; D016130:Immunophenotyping; D007694:Killer Cells, Natural / Q000473:pathology; D016246:Lymphocytes, Tumor-Infiltrating / Q000473:pathology*; D016483:Lymphoma, AIDS-Related / Q000473:pathology*; D016393:Lymphoma, B-Cell / Q000473:pathology*; D013601:T-Lymphocytes / Q000473:pathology*; D006377:T-Lymphocytes, Helper-Inducer / Q000473:pathology; D050378:T-Lymphocytes, Regulatory / Q000473:pathology
chemical_list: D015703:Antigens, CD