title: Synthesis and properties of oligonucleotides containing the mutagenic base O4-benzylthymidine.
abstract: The preparation of synthetic oligodeoxynucleotides containing O4-benzylthymidine (Tbn) is described. The use of standard and t-butylphenoxyacetyl amino protecting groups is compared. The thermal stabilities of duplexes containing Tbn paired with adenine and guanine have been measured.
authors.affiliation: Department of Molecular Genetics, CID-CSIC, Barcelona, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Fabrega
authors.forename: R
authors.initials: R
authors.lastname: Eritja
authors.forename: N D
authors.initials: ND
authors.lastname: Sinha
authors.forename: M K
authors.initials: MK
authors.lastname: Dosanjh
authors.forename: B
authors.initials: B
authors.lastname: Singer
mesh_terms: D001482:Base Composition; D001483:Base Sequence; D002942:Circular Dichroism; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009841:Oligonucleotides / Q000138:chemical synthesis*; D009943:Organophosphorus Compounds / Q000737:chemistry; D013936:Thymidine / Q000031:analogs & derivatives*
chemical_list: D009841:Oligonucleotides; D009943:Organophosphorus Compounds; D013936:Thymidine