Identification of a promoter region in the rat prion protein gene.
We have demonstrated the presence of a rat prion protein (RaPrP) gene promoter upstream of multiple initiation sites. A 0.1-kb fragment upstream of the 5'-untranslated region contains specific DNA motifs characteristic of promoter elements including an AP-1 binding site, an inverted CCAAT motif and three inverted Sp-1 binding sites. This fragment directs transcription of a luciferase reporter gene in pheochromocytoma cells (PC12) and rat glioma cells (C6), suggesting that it contains the promoter for the RaPrP gene. To more precisely localize the transcription regulatory elements in this region, a series of 5'-deletion mutants were generated. Deletion analysis showed that an inverted CCAAt and adjoining Sp-1 binding sequences may play an important role in transcription of the RaPrP gene.
Saeki
K
K
Department of Molecular Immunology, Faculty of Agriculture, University, of Tokyo, Japan.
Matsumoto
Y
Y
Matsumoto
Y
Y
Onodera
T
T
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D006224:Cricetinae; D017931:DNA Primers; D005091:Exons; D015698:Genomic Library; D005910:Glioma; D007438:Introns; D008099:Liver / Q000378:metabolism; D008156:Luciferases / Q000032:analysis / Q000096:biosynthesis; D051379:Mice; D008969:Molecular Sequence Data; D016716:PC12 Cells; D016133:Polymerase Chain Reaction; D011328:Prions / Q000235:genetics*; D011401:Promoter Regions, Genetic; D051381:Rats; D011994:Recombinant Proteins / Q000032:analysis / Q000096:biosynthesis; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000378:metabolism; D014162:Transfection; D014407:Tumor Cells, Cultured
D017931:DNA Primers; D011328:Prions; D011994:Recombinant Proteins; D014157:Transcription Factors; D008156:Luciferases