title: Lack of mutation in tumour-suppressor gene p53 in gestational trophoblastic tumours.
abstract: The objectives of this study were to better our understanding of the carcinogenesis of gestational trophoblastic tumours and to investigate the possible presence of mutational alteration of the p53 tumour-suppressor gene in these tumours. Amplification-based direct DNA sequencing was performed on 14 hydatidiform moles, six invasive moles, eight choriocarcinomas and ten normal early placental tissues. No mutation in exons 5-8 was detected in any of these 38 tissue specimens. These results suggest that a mutation in p53 tumour suppressor either does not exist or is a very rare event in gestational trophoblastic tumours. The gestational trophoblastic tumours probably involve a tumour-suppressor gene other than p53 gene or may follow a completely different pathway to their malignant phenotype.
authors.affiliation: Women's Hospital, Zhejiang Medical University, Hangzhou, China.
authors.forename: Y F
authors.initials: YF
authors.lastname: Shi
authors.forename: X
authors.initials: X
authors.lastname: Xie
authors.forename: C L
authors.initials: CL
authors.lastname: Zhao
authors.forename: D F
authors.initials: DF
authors.lastname: Ye
authors.forename: S M
authors.initials: SM
authors.lastname: Lu
authors.forename: J J
authors.initials: JJ
authors.lastname: Hor
authors.forename: C C
authors.initials: CC
authors.lastname: Pao
mesh_terms: D000818:Animals; D001483:Base Sequence; D002467:Cell Nucleus / Q000378:metabolism; D002822:Choriocarcinoma / Q000235:genetics*; D017931:DNA Primers / Q000737:chemistry; D005260:Female; D016158:Genes, p53; D006828:Hydatidiform Mole / Q000235:genetics*; D002820:Hydatidiform Mole, Invasive / Q000235:genetics*; D008969:Molecular Sequence Data; D017354:Point Mutation; D011247:Pregnancy; D014327:Trophoblasts / Q000502:physiology; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*
chemical_list: D017931:DNA Primers; D016159:Tumor Suppressor Protein p53