Sphingosine-1-phosphate inhibits actin nucleation and pseudopodium formation to control cell motility of mouse melanoma cells.
Sphingosine-1-phosphate (Sph-1-P), the initial product of sphingosine (Sph) catabolism, has been reported to inhibit motility of mouse melanoma B16/F1 and other types of cells at very low concentrations (10-100 nM). Sph-1-P (100 nM-1 microM) inhibited pseudopodium formation by blocking polymerization and reorganization of actin filaments in newly formed pseudopodia, and reduced F-actin by approximately 25% in F1 cells. A pyrene-labeled actin nucleation assay revealed that Sph-1-P (100 nM) inhibits actin nucleation mediated by F1 cell plasma membranes. These results suggest that Sph-1-P interacts with molecules associated with actin nucleation to inhibit reorganization of pseudopodium formation and cell motility.
Yamamura
S
S
The Biomembrane Institute, Seattle, WA 98119, USA.
Sadahira
Y
Y
Ruan
F
F
Hakomori
S
S
Igarashi
Y
Y
D000199:Actins / Q000378:metabolism*; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D002465:Cell Movement / Q000187:drug effects*; D017077:Culture Media, Conditioned / Q000494:pharmacology; D008246:Lysophospholipids; D008545:Melanoma; D051379:Mice; D011554:Pseudopodia / Q000187:drug effects*; D013110:Sphingosine / Q000031:analogs & derivatives* / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D000199:Actins; D017077:Culture Media, Conditioned; D008246:Lysophospholipids; C060506:sphingosine 1-phosphate; D013110:Sphingosine; D002118:Calcium