Successful induction of severe destructive arthritis by the transfer of in vitro-activated synovial fluid T cells from patients with rheumatoid arthritis (RA) in severe combined immunodeficient (SCID) mice.
In order to investigate the role of pathogenic T cells in RA, the establishment of an RA model using patients' T cells is thought to be essential. In this study, multiple and severe destructive arthritis was established by transferring in vitro-stimulated synovial fluid T (SFT) cells from patients with RA through simultaneous injection into knee joint and peritoneal cavity of SCID mice without causing xenogeneic graft-versus-host disease (GVHD). Neither the transfer of unstimulated SFT cells nor sole i.p. injection was sufficient to induce severe arthritis. Interestingly, in contrast with SFT cells, in vitro-activated peripheral blood lymphocytes from RA patients failed to trigger such arthritis, suggesting that pathogenic T cells might be concentrated in synovial fluid of RA patients. This, the first severe arthritis model mimicking RA induced by RA patients' T cells, is expected to provide important information about RA pathogenesis and a possible therapeutic approach.
Sakata
A
A
Department of Immunology, Kumamoto University School of Medicine, Japan.
Sakata
K
K
Ping
H
H
Ohmura
T
T
Tsukano
M
M
Kakimoto
K
K
D000368:Aged; D000818:Animals; D000954:Antigens, Surface / Q000032:analysis; D001168:Arthritis / Q000209:etiology* / Q000276:immunology*; D001172:Arthritis, Rheumatoid / Q000276:immunology*; D004195:Disease Models, Animal; D005260:Female; D006086:Graft vs Host Disease / Q000209:etiology; D006801:Humans; D007150:Immunohistochemistry; D007963:Leukocytes, Mononuclear / Q000637:transplantation; D008213:Lymphocyte Activation; D017710:Lymphocyte Transfusion; D008297:Male; D051379:Mice; D016513:Mice, SCID; D008875:Middle Aged; D013582:Synovial Fluid / Q000276:immunology*; D013601:T-Lymphocytes / Q000145:classification / Q000276:immunology / Q000637:transplantation
D000954:Antigens, Surface