Monocyte chemoattractant protein-1 levels in patients with glomerular disease.
Chemokines such as monocyte chemoattractant protein-1 (MCP-1) and interleukin-8 have been implicated in recruiting leukocytes to the glomerulus during immune renal injury. To detect the expression of MCP-1 in human glomerular disease, we measured urinary MCP-1 levels in patients with a variety of glomerulopathies. These data demonstrated that MCP-1 was present in the urine of patients with glomerular disease and that patients with inflammatory glomerulopathies had higher levels of urinary MCP-1. Urinary MCP-1 correlated with the extent of proteinuria (r = 0.71, P < 0.0001), but not with serum MCP-1 levels (r = 0.14, P > 0.3). The MCP-1 present in urine was biologically active, increasing monocyte migration in a microchemotaxis assay. This activity was attenuated by the addition of anti-MCP-1 antibody to the samples. Enumerating glomerular macrophages demonstrated that glomerular inflammation was correlated with urinary MCP-1 levels (r = 0.53, P < 0.02). Individuals with light microscopic evidence of severe glomerular injury (ie, crescents, necrosis, endocapillary proliferation) had significantly higher levels of MCP-1 in their urine than patients with less severe glomerular changes (1,962 +/- 612 pg MCP-1/mg creatinine v 314 +/- 45 pg MCP-1/mg creatinine; P < 0.002). Taken together, these results suggest that urinary MCP-1 reflects the extent to which MCP-1 that is produced in the glomerulus participates in the glomerular inflammatory response.
Rovin
B H
BH
Department of Medicine, Ohio State University, Columbus, 43210, USA.
Doe
N
N
Tan
L C
LC
D018932:Chemokine CCL2 / Q000097:blood / Q000652:urine*; D002634:Chemotaxis, Leukocyte; D003404:Creatinine / Q000652:urine; D005260:Female; D005921:Glomerulonephritis / Q000097:blood / Q000473:pathology / Q000652:urine*; D006801:Humans; D016209:Interleukin-8 / Q000652:urine; D007678:Kidney Glomerulus / Q000276:immunology / Q000473:pathology; D008262:Macrophage Activation; D008264:Macrophages / Q000473:pathology; D008297:Male; D009336:Necrosis; D011507:Proteinuria / Q000652:urine
D018932:Chemokine CCL2; D016209:Interleukin-8; D003404:Creatinine