Naturally occurring modified low density lipoproteins are similar if not identical: more electronegative and desialylated lipoprotein subfractions.
Previous studies demonstrated that more electronegative low density lipoprotein (LDL) isolated from human blood by ion exchange chromatography has a chemical composition and physical properties similar to desialylated LDL obtained by lectin chromatography (Avogaro et al., 1988; Orekhov et al., 1989). In this study, sialic acid content and percentage of desialylated LDL in the electronegative LDL (LDL-) subfraction have been investigated. The sialic acid content of the LDL- was 2- to 6-fold lower than that of native LDL and close to that of desialylated LDL. In the native LDL subfraction, 83% of lipoprotein particles did not bind to the Ricinus communis agglutinin, indicating lack of terminal galactose, presumably appearing as a result of desialylation of LDL carbohydrate chains. By contrast, a major proportion of human LDL- (81%) was bound to the lectin. It was also found that the desialylated LDL subfraction consists of 88% LDL-. Native LDL did not affect the contents of free and esterified cholesterol in intimal cells cultured from grossly normal human aorta, while LDL- and desialylated LDL induced a 1.5- to 3-fold increase in the intracellular content of cholesteryl esters. Thus, LDL- is desialylated LDL which induces lipid accumulation in intimal cells. Our findings suggest that the LDL- particle is similar if not identical to the desialylated LDL particle.
Tertov
V V
VV
Institute of Experimental Cardiology, Moscow, Russia.
Bittolo-Bon
G
G
Sobenin
I A
IA
Cazzolato
G
G
Orekhov
A N
AN
Avogaro
P
P
D000328:Adult; D000368:Aged; D001011:Aorta / Q000737:chemistry; D001665:Binding Sites; D002478:Cells, Cultured; D005591:Chemical Fractionation; D002784:Cholesterol / Q000032:analysis; D002788:Cholesterol Esters / Q000032:analysis; D002852:Chromatography, Ion Exchange; D003324:Coronary Artery Disease / Q000097:blood; D006801:Humans; D008077:Lipoproteins, LDL / Q000097:blood / Q000737:chemistry*; D008297:Male; D008875:Middle Aged; D009131:Muscle, Smooth, Vascular / Q000737:chemistry / Q000166:cytology; D012794:Sialic Acids / Q000032:analysis* / Q000097:blood; D017539:Tunica Intima / Q000737:chemistry
D002788:Cholesterol Esters; D008077:Lipoproteins, LDL; D012794:Sialic Acids; D002784:Cholesterol