Identification of Tnr3, a suppressor-mutator/enhancer-like transposable element from rice.
We isolated members of the retroposon family p-SINE1 in rice and found that one member contained an insertion.Aa 3-bp sequence at the insertion site within p-SINE1 appeared duplicated. The insertion sequence, 1539 bp in length, carried imperfect inverted repeats of about 13 bp at its termini which begin with 5'-CACTA---3'; these repeats are similar to those found in members of the En/Spm transposable element family. These results indicate that the insertion sequence is a transposable element belonging to the En/Spm family and is thus named Tnr3 (transposable element in rice no. 3). In fact, Tnr carried long subterminal regions containing direct and inverted repeats of short DNA sequences of 15 bp, another characteristic of the En/Spm family. The subterminal repeat sequences in Tnr3 are, however, of two kinds, although they share homology with each other. Tnr3 and its relatives were present in multiple copies in rice. considering the length of Tnr3, it cannot represent an autonomous type element, but is a non-autonomous element probably derived by deletion from an autonomous transposon.
Motohashi
R
R
Institute of Molecular and Cellular Biosciences, University of Tokyo, Japan.
Ohtsubo
E
E
Ohtsubo
H
H
D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D017931:DNA Primers / Q000737:chemistry; D004251:DNA Transposable Elements / Q000235:genetics*; D004742:Enhancer Elements, Genetic / Q000235:genetics*; D008969:Molecular Sequence Data; D012275:Oryza / Q000737:chemistry / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012091:Repetitive Sequences, Nucleic Acid; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013489:Suppression, Genetic / Q000235:genetics*
D017931:DNA Primers; D004251:DNA Transposable Elements