Behavior of the cell cycle-associated proteins in an unusual G0-arrestable cancer cell line.
An adenocarcinoma cell line which has the ability to arrest in G0 phase under exhausted culture conditions was established. Using the cell line, we investigated the expression of cell cycle-associated proteins including cdc2, cdk2, cyclin A, cyclin Dl, and Rb during entry into or withdrawal from the cell cycle. MAP kinase expression was also investigated as one of the most downstream proteins of the signal transduction of growth factors. The cells in the quiescent state did not express cdc2. In contrast, cdk2 was expressed weakly, and cyclin A and cyclin D1 were strongly expressed in the quiescent cells. The expression of cdk2 and cyclin D1 in the quiescent cells was reduced after stimulation by renewal of the medium and then increased, accompanied by Rb phosphorylation and cdc2 expression around the G1/S transition. Cdc2 and the hyperphosphorylated form of Rb disappeared as the cells became quiescent. MAP kinase expression was unchanged throughout all the phases analyzed. The results indicate that down-regulation of neither cdk2, cyclin A, cyclin D1, nor MAP kinase is necessary to arrest cells in G0, but that only Rb dephosphorylation and down-regulation of cdc2 are accompanied by an arrest of cell proliferation in G0 in the cell line.
Ohta
T
T
First Department of Surgery, St. Marianna University School of Medicine, Kawasaki, Japan.
Fukuda
M
M
Wanebo
H J
HJ
Jogo
K
K
Yamaguchi
S
S
D000230:Adenocarcinoma / Q000378:metabolism / Q000473:pathology; D000368:Aged; D000818:Animals; D016203:CDC2 Protein Kinase / Q000096:biosynthesis; D042846:CDC2-CDC28 Kinases; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000096:biosynthesis; D002453:Cell Cycle / Q000502:physiology*; D051357:Cyclin-Dependent Kinase 2; D018844:Cyclin-Dependent Kinases / Q000096:biosynthesis; D016213:Cyclins / Q000096:biosynthesis; D005260:Female; D006801:Humans; D007621:Karyotyping; D008175:Lung Neoplasms / Q000378:metabolism / Q000473:pathology; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D017346:Protein Serine-Threonine Kinases / Q000096:biosynthesis; D016192:Resting Phase, Cell Cycle; D016160:Retinoblastoma Protein / Q000096:biosynthesis; D013997:Time Factors; D014407:Tumor Cells, Cultured / Q000166:cytology / Q000378:metabolism
D016213:Cyclins; D016160:Retinoblastoma Protein; D017346:Protein Serine-Threonine Kinases; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D016203:CDC2 Protein Kinase; D042846:CDC2-CDC28 Kinases; C495894:CDK2 protein, human; C495893:Cdk2 protein, mouse; D051357:Cyclin-Dependent Kinase 2; D018844:Cyclin-Dependent Kinases