In vivo drug screening applications of HIV-infected cells cultivated within hollow fibers in two physiologic compartments of mice.
Previous studies demonstrated that human cell lines can be cultivated in hollow fibers in the subcutaneous and intraperitoneal compartments of mice. We have extended the range of cell lines to include cells infected with the human immunodeficiency virus (HIV). Furthermore, these HIV-infected cells have been shown to replicate in the hollow fibers located in both physiologic compartments (intraperitoneal and subcutaneous) of SCID mice. Treatment of the host mice with antiviral agents can suppress virus replication in these hollow fiber cultures. The potential use of this system for early in vivo screening of anti-HIV compounds is discussed.
Hollingshead
M
M
Biological Testing Branch, DTP, DCT, NCI, Fairview Center, Frederick, MD 21701, USA.
Roberson
J
J
Decker
W
W
Buckheit
R
R
Elder
C
C
Malspeis
L
L
Mayo
J
J
Grever
M
M
D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D006678:HIV / Q000187:drug effects*; D016655:HIV Core Protein p24 / Q000032:analysis; D006801:Humans; D051379:Mice; D016513:Mice, SCID; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D014776:Virus Cultivation; D014779:Virus Replication / Q000187:drug effects; D016047:Zalcitabine / Q000494:pharmacology; D015215:Zidovudine / Q000494:pharmacology
D000998:Antiviral Agents; D016655:HIV Core Protein p24; D015215:Zidovudine; D016047:Zalcitabine; D012194:RNA-Directed DNA Polymerase