title: Structure and function of a novel voltage-gated, tetrodotoxin-resistant sodium channel specific to sensory neurons.
abstract: Small neurons of the dorsal root ganglia (DRG) are known to play an important role in nociceptive mechanisms. These neurons express two types of sodium current, which differ in their inactivation kinetics and sensitivity to tetrodotoxin. Here, we report the cloning of the alpha-subunit of a novel, voltage-gated sodium channel (PN3) from rat DRG. Functional expression in Xenopus oocytes showed that PN3 is a voltage-gated sodium channel with a depolarized activation potential, slow inactivation kinetics, and resistance to high concentrations of tetrodotoxin. In situ hybridization to rat DRG indicated that PN3 is expressed primarily in small sensory neurons of the peripheral nervous system.
authors.affiliation: Institute of Pharmacology, Neurobiology Unit, Roche Bioscience, Palo Alto, CA 94304, USA.
authors.forename: L
authors.initials: L
authors.lastname: Sangameswaran
authors.forename: S G
authors.initials: SG
authors.lastname: Delgado
authors.forename: L M
authors.initials: LM
authors.lastname: Fish
authors.forename: B D
authors.initials: BD
authors.lastname: Koch
authors.forename: L B
authors.initials: LB
authors.lastname: Jakeman
authors.forename: G R
authors.initials: GR
authors.lastname: Stewart
authors.forename: P
authors.initials: P
authors.lastname: Sze
authors.forename: J C
authors.initials: JC
authors.lastname: Hunter
authors.forename: R M
authors.initials: RM
authors.lastname: Eglen
authors.forename: R C
authors.initials: RC
authors.lastname: Herman
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D004351:Drug Resistance; D005260:Female; D005727:Ganglia, Spinal / Q000378:metabolism; D017403:In Situ Hybridization; D066298:In Vitro Techniques; D015640:Ion Channel Gating; D008297:Male; D008564:Membrane Potentials; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009475:Neurons, Afferent / Q000378:metabolism*; D009865:Oocytes; D017933:Peripheral Nervous System / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D015222:Sodium Channels / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D013779:Tetrodotoxin / Q000494:pharmacology; D014981:Xenopus
chemical_list: D015222:Sodium Channels; D013779:Tetrodotoxin