A factor derived from polymorphonuclear leukocytes enhances interleukin-1-induced synovial cell collagenase and prostaglandin E2 production in rats.
We found that short-term culture medium and homogenate of casein-induced rat peritoneal polymorphonuclear leukocytes (PMN) markedly induced collagenase and prostaglandin E2 (PGE2) production by normal rat synovial cells and these effects were abrogated by anti-(rat interleukin-1 alpha) (IL-1 alpha) polyclonal antibodies. However, collagenase activity and PGE2 induced by recombinant rat IL-1 alpha were less than those induced by rat PMN culture medium. It was also proved by radioimmunoassay that rat PMN culture medium contains a relatively small amount of IL-1 alpha. The introduction of IL-1 alpha-deleted PMN culture medium and recombinant rat IL-1 alpha together into the synovial cell culture system revealed that IL-1 alpha deleted PMN culture medium has a significant enhancing activity on IL-1 alpha-induced synovial cell collagenase and PGE2 production. This new factor, which was shown to be a negatively charged protein of about 80 kDa, may have important roles in connective tissue destruction and chronic inflammation in diseases such as rheumatoid arthritis.
Hashida
R
R
Tsukuba Research Laboratories, Eisai Co., Ltd., Japan.
Kuwada
M
M
Chiba
K I
KI
Horizoe
T
T
Shirota
H
H
Nagai
Y
Y
D000818:Animals; D002364:Caseins / Q000494:pharmacology; D017364:Collagenases / Q000378:metabolism*; D015232:Dinoprostone / Q000096:biosynthesis*; D007375:Interleukin-1 / Q000008:administration & dosage*; D008297:Male; D009504:Neutrophils / Q000502:physiology*; D051381:Rats; D011917:Rats, Inbred Lew; D011994:Recombinant Proteins; D013583:Synovial Membrane / Q000201:enzymology*
D002364:Caseins; D007375:Interleukin-1; D011994:Recombinant Proteins; D017364:Collagenases; D015232:Dinoprostone