Differential spatiotemporal expression of E- and P-cadherin during mouse tooth development.
Changes in E- and P-cadherin (E- and P-CD) expression during embryonic mouse first molar development were analyzed by immunohistochemistry. During the induction and morphogenesis stages (bud, cap and early bell stages), E-CD was expressed in the cells of the invaginating epithelial tooth bud and in the cells of the outer enamel epithelium, stellate reticulum and stratum intermedium, suggesting a role for this molecule in the maintenance of enamel organ architecture. On the other hand, P-CD was strongly expressed in the inner enamel epithelium suggesting its participation in the processes of mesenchymal induction. during the cytodifferentiation stage (late bell stage), E-CD was expressed in polarizing preameloblasts, but cadherin expression was restricted to the basal and apical poles of differentiated secretory ameloblasts, where the zonula adherens type of cell-cell junctions is located. The present study demonstrates for the first time the spatiotemporal expression of cadherins during tooth development and suggests differential and specific roles for E-CD and P-CD during the morphogenesis and cytodifferentiation processes of this organ.
Palacios
J
J
Departamento de Anatomía Patológica, Hospital La Paz, Madrid, Spain.
Benito
N
N
Berraquero
R
R
Pizarro
A
A
Cano
A
A
Gamallo
C
C
D000818:Animals; D000911:Antibodies, Monoclonal; D015820:Cadherins / Q000032:analysis / Q000096:biosynthesis*; D003743:Dental Enamel / Q000166:cytology / Q000196:embryology; D005314:Embryonic and Fetal Development; D004848:Epithelium / Q000502:physiology; D005260:Female; D015870:Gene Expression; D005865:Gestational Age; D007150:Immunohistochemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D008963:Molar / Q000166:cytology / Q000196:embryology*; D009805:Odontogenesis; D011247:Pregnancy
D000911:Antibodies, Monoclonal; D015820:Cadherins