Isolation of a genomic DNA fragment having negative vitamin D response element.
The vitamin D receptor (VDR) binds to the vitamin D response element (VDRE) in the promoter region of target genes and acts as a ligand-dependent transcriptional regulator. In order to identify novel VDREs and new genes that are regulated by the active form of vitamin D [1,25-(OH)2D3], rat genomic DNA fragments bound by VDR were isolated. One of these fragments, designated as VBF5 was transcribed and the transcript was down-regulated by 1,25-(OH)2D3. These data strongly indicate that VBF5 may contain a VDRE regulating negatively an unidentified gene expression.
Sakoda
K
K
Department of Environmental Biochemistry, Faculty of Pharmaceutical Sciences, Osaka University, Japan.
Fujiwara
M
M
Arai
S
S
Suzuki
A
A
Nishikawa
J
J
Imagawa
M
M
Nishihara
T
T
D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002460:Cell Line; D003001:Cloning, Molecular; D016207:Cytokines / Q000096:biosynthesis / Q000235:genetics; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000378:metabolism*; D007668:Kidney / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000737:chemistry* / Q000378:metabolism; D053495:Osteopontin; D011401:Promoter Regions, Genetic; D051381:Rats; D018167:Receptors, Calcitriol / Q000096:biosynthesis / Q000378:metabolism*; D012045:Regulatory Sequences, Nucleic Acid; D012795:Sialoglycoproteins / Q000096:biosynthesis / Q000235:genetics; D013154:Spleen / Q000378:metabolism; D014158:Transcription, Genetic; D014162:Transfection; D014407:Tumor Cells, Cultured
D016207:Cytokines; D004268:DNA-Binding Proteins; D009838:Oligodeoxyribonucleotides; D018167:Receptors, Calcitriol; D012795:Sialoglycoproteins; C506896:Spp1 protein, mouse; C506897:Spp1 protein, rat; D053495:Osteopontin; D004247:DNA