title: REGA-1 is a GPI-linked member of the immunoglobulin superfamily present on restricted regions of sheath cell processes in grasshopper.
abstract: REGA-1 is a glycoprotein localized to sheath cell processes in the developing CNS when NBs are producing progeny and neurons are maturing and extending processes. It is also present on a subset of muscles and on the lumenal surface of the ectoderm in the embryonic appendages when pioneer neurons are growing into the CNS. REGA-1 is associated with the extracellular side of the cell membrane by a glycosyl-phosphatidylinositol linkage. We have identified a cDNA clone encoding REGA-1 using a sequence from purified protein. Sequence analysis defines REGA-1 as a novel member of the immunoglobulin superfamily containing three immunoglobulin domains and one fibronectin type III repeat. Each Ig domain has distinct sequence characteristics that suggest discrete functions. REGA-1 is similar to other Ig superfamily members involved in cell adhesion events and neurite outgrowth.
authors.affiliation: Department of Biology, University of Utah, Salt Lake City, 84112, USA.
authors.forename: E C
authors.initials: EC
authors.lastname: Seaver
authors.forename: E M
authors.initials: EM
authors.lastname: Carpenter
authors.forename: M J
authors.initials: MJ
authors.lastname: Bastiani
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002846:Chromatography, Affinity; D003001:Cloning, Molecular; D017124:Conserved Sequence; D017931:DNA Primers; D018076:DNA, Complementary; D004625:Embryo, Nonmammalian / Q000166:cytology* / Q000502:physiology*; D005353:Fibronectins / Q000737:chemistry; D017261:Glycosylphosphatidylinositols / Q000378:metabolism*; D006110:Grasshoppers / Q000196:embryology*; D007136:Immunoglobulins / Q000096:biosynthesis / Q000737:chemistry*; D007150:Immunohistochemistry; D019476:Insect Proteins; D008562:Membrane Glycoproteins / Q000032:analysis / Q000096:biosynthesis* / Q000737:chemistry*; D051379:Mice; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000096:biosynthesis* / Q000737:chemistry*; D009928:Organ Specificity; D016133:Polymerase Chain Reaction; D017433:Protein Structure, Secondary; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D005353:Fibronectins; D017261:Glycosylphosphatidylinositols; D007136:Immunoglobulins; D019476:Insect Proteins; D008562:Membrane Glycoproteins; D009419:Nerve Tissue Proteins; C098695:REGA-1 protein, Schistocerca americana