title: The molecular basis for the natural resistance of the cytochrome bc1 complex from strobilurin-producing basidiomycetes to center Qp inhibitors.
abstract: Mitochondria from the strobilurin A producing basidiomycetes Strobilurus tenacellus and Mycena galopoda exhibit natural resistance to (E)-beta-methoxyacrylate inhibitors of the ubiquinol oxidation center(center Qp) of the cytochrome bc1 complex. Isolated cytochrome bc1 complex from S. tenacellus was found to be highly similar to that of Saccharomyces cerevisiae with respect to subunit composition, as well as spectral characteristics and midpoint potentials of the heme centers. To understand the molecular basis of natural resistance, we determined the exon/intron organization and deduced the sequences of cytochromes b from S. tenacellus, M. galopoda and a third basidiomycete, Mycena viridimarginata, which produces no strobilurin A. Comparative sequence analysis of two regions of cytochrome b known to contribute to the formation of center Qp suggested that the generally lower sensitivity of all three basidiomycetes was due to the replacement of a small amino acid residue in position 127 by isoleucine. For M. galopoda replacement of Gly143 by alanine and Gly153 by serine, for S. tenacellus replacement of a small residue in position 254 by glutamine and Asn261 by aspartate was found to be the likely causes for resistance to (E)-beta-methoxyacrylates. The latter exchange is also found in Schizosaccharomyces pombe, which we found also to be naturally resistant to (E)-beta-methoxyacrylates.
authors.affiliation: Universitätsklinikum Frankfurt, Zentrum der Biologischen Chemie, Frankfurt am Main, Germany.
authors.forename: P
authors.initials: P
authors.lastname: Kraiczy
authors.forename: U
authors.initials: U
authors.lastname: Haase
authors.forename: S
authors.initials: S
authors.lastname: Gencic
authors.forename: S
authors.initials: S
authors.lastname: Flindt
authors.forename: T
authors.initials: T
authors.lastname: Anke
authors.forename: U
authors.initials: U
authors.lastname: Brandt
authors.forename: G
authors.initials: G
authors.lastname: Von Jagow
mesh_terms: D000595:Amino Acid Sequence; D000935:Antifungal Agents / Q000096:biosynthesis*; D001487:Basidiomycota / Q000235:genetics / Q000378:metabolism*; D002352:Carrier Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D003001:Cloning, Molecular; D016384:Consensus Sequence; D003573:Cytochrome b Group / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics; D004272:DNA, Mitochondrial / Q000235:genetics; D014450:Electron Transport Complex III / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D005231:Fatty Acids, Unsaturated / Q000096:biosynthesis / Q000494:pharmacology; D005800:Genes, Fungal; D008689:Methacrylates; D008928:Mitochondria / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D010101:Oxygen Consumption; D017510:Protein Folding; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D000073739:Strobilurins; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000037:antagonists & inhibitors / Q000378:metabolism
chemical_list: D000935:Antifungal Agents; D002352:Carrier Proteins; D003573:Cytochrome b Group; D004271:DNA, Fungal; D004272:DNA, Mitochondrial; D005231:Fatty Acids, Unsaturated; D008689:Methacrylates; D000073739:Strobilurins; C027208:ubiquinone-binding proteins; D014451:Ubiquinone; C008556:mucidin; D014450:Electron Transport Complex III; C003741:ubiquinol