title: The 4-kDa nuclear-encoded PetM polypeptide of the chloroplast cytochrome b6f complex. Nucleic acid and protein sequences, targeting signals, transmembrane topology.
abstract: The 4-kDa subunit of cytochrome b6f complex encoded by the nuclear PetM gene in Chlamydomonas reinhardtii has been characterized. 38 of the 39 residues of the mature protein have been established by Edman degradation, a cDNA clone encoding the complete precursor has been isolated and sequenced, and a 0.6-kb transcript detected. The deduced amino acid sequence of the precursor includes an N-terminal transit peptide of 60 amino acids with stromal targeting features. Examination of the sequence suggests that PetM spans the membrane as a single transmembrane alpha-helix, which is supported by its non-extractability following dissociating treatments. When PetM and PetG, another small subunit of the b6f complex, are folded into alpha-helices, an array of identical residues becomes apparent. Proteolysis data, charge distribution, and homology with PetG are consistent with a lumenal localization of the N terminus of PetM.
authors.affiliation: Service de Photosynthèse, CNRS/URA 1187, Institut de Biologie Physico-Chimique, Paris, France.
authors.forename: C
authors.initials: C
authors.lastname: de Vitry
authors.forename: C
authors.initials: C
authors.lastname: Breyton
authors.forename: Y
authors.initials: Y
authors.lastname: Pierre
authors.forename: J L
authors.initials: JL
authors.lastname: Popot
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002462:Cell Membrane / Q000378:metabolism; D002736:Chloroplasts / Q000201:enzymology*; D003573:Cytochrome b Group / Q000737:chemistry*; D045346:Cytochrome b6f Complex; D018076:DNA, Complementary; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D010940:Plant Proteins; D011487:Protein Conformation
chemical_list: D003573:Cytochrome b Group; D018076:DNA, Complementary; D009687:Nuclear Proteins; C098901:PetM protein, Chlamydomonas reinhardtii; D010940:Plant Proteins; D045346:Cytochrome b6f Complex