Species differences in hepatic bile acid uptake: comparative evaluation of taurocholate and tauroursodeoxycholate extraction in rat and rabbit.
Dose-response curves for taurocholate and tauroursodeoxycholate were performed in rat and rabbit livers to get more insight into species differences in the hepatic bile acid uptake. The bile acids showed saturation kinetics in both animals, the Vmax in rat being higher than in rabbit and the Km being lower in the rat than in the rabbit for both the bile acids, with no significant difference in the hepatic cells morphometric parameters. Therefore, it is possible that differences in the kinetic parameters are related to the number and, to a lesser extent, to the affinity of the transporters on the sinusoidal plasma membranes.
Montagnani
M
M
Cattedra di Gastroenterologia, Università di Bologna, Italy.
Aldini
R
R
Roda
A
A
Caruso
M L
ML
Gioacchini
A M
AM
Lenzi
P L
PL
Roda
E
E
D000818:Animals; D001647:Bile Acids and Salts / Q000378:metabolism*; D066298:In Vitro Techniques; D008099:Liver / Q000033:anatomy & histology / Q000166:cytology / Q000378:metabolism*; D008297:Male; D009929:Organ Size / Q000502:physiology; D010477:Perfusion; D011169:Portal Vein / Q000378:metabolism; D011817:Rabbits; D051381:Rats; D017207:Rats, Sprague-Dawley; D013045:Species Specificity; D013655:Taurochenodeoxycholic Acid / Q000378:metabolism*; D013656:Taurocholic Acid / Q000378:metabolism*
D001647:Bile Acids and Salts; D013655:Taurochenodeoxycholic Acid; D013656:Taurocholic Acid; C031655:ursodoxicoltaurine