Characterization of dp6troglycan-laminin interaction in peripheral nerve.
Dystoroglycan is encoded by a single gene and cleaved into two proteins, alpha and beta-dystroglycan, by posttranslational processing. The 120kDa peripheral nerve isoform of alpha-dystroglycan binds laminin-2 comprised of the alpha 2, beta 1, and gamma 1 chains. In congenital muscular dystrophy and dy mice deficient in laminin alpha 2 chain, peripheral myelination is disturbed, suggesting a role for the dystroglycan- laminin interaction in peripheral myelinogenesis. To begin to test this hypothesis, we have characterized the dystroglycan-laminin interaction in peripheral nerve. We demonstrate that (1) alpha-dystroglycan is an extracellular peripheral membrane glycoprotein that links beta-dystroglycan in the Schwann cell outer membrane with laminin-2 in the endoneurial basal lamina, and (2) dystrophin homologues Dp116 and utrophin are cytoskeletal proteins of the Schwann cell cytoplasm. We also present data that suggest a role for glycosylation of alpha-dystroglycan in the interaction with laminin.
Yamada
H
H
Department of Neurology and Neuroscience, Teikyo University School of Medicine, Tokyo, Japan.
Chiba
A
A
Endo
T
T
Kobata
A
A
Anderson
L V
LV
Hori
H
H
Fukuta-Ohi
H
H
Kanazawa
I
I
Campbell
K P
KP
Shimizu
T
T
Matsumura
K
K
D000818:Animals; D002241:Carbohydrates / Q000502:physiology; D002417:Cattle; D003598:Cytoskeletal Proteins / Q000378:metabolism* / Q000648:ultrastructure; D049030:Dystroglycans; D016189:Dystrophin / Q000378:metabolism* / Q000648:ultrastructure; D007797:Laminin / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000378:metabolism* / Q000648:ultrastructure; D010525:Peripheral Nerves / Q000378:metabolism*; D011817:Rabbits; D012584:Sciatic Nerve / Q000378:metabolism*
D002241:Carbohydrates; D003598:Cytoskeletal Proteins; D016189:Dystrophin; D007797:Laminin; D008562:Membrane Glycoproteins; D049030:Dystroglycans