title: Perfluorocarbon-associated gas exchange improves oxygenation, lung mechanics, and survival in a model of adult respiratory distress syndrome.
abstract: OBJECTIVE
  To compare the effectiveness of perfluorocarbon-associated gas exchange to volume controlled positive pressure breathing in supporting gas exchange, lung mechanics, and survival in an acute lung injury model.
  
  
  METHODS
  A prospective, randomized study.
  
  
  METHODS
  A university medical school laboratory approved for animal research.
  
  
  METHODS
  Neonatal piglets.
  
  
  METHODS
  Eighteen piglets were randomized to receive perfluorcarbon-associated gas exchange with perflubron (n=10) or volume controlled continuous positive pressure breathing (n=8) after acute lung injury was induced by oleic acid infusion (0.15 mL/kg iv).
  
  
  RESULTS
  Arterial and venous blood gases, hemodynamics, and lung mechanics were measured every 15 mins during a 3-hr study period. All animals developed a metabolic and a respiratory acidosis during the infusion of oleic acid. Following randomization, the volume controlled positive pressure breathing group developed a profound acidosis (p<.05), while pH did not change in the perfluorocarbon-associated gas exchange group. Within 15 mins of initiating perfluorocarbon-associated gas exchange, oxygenation increased from a PaO2 of 52 +/- 12 torr (6.92 +/- 1.60 kPa) to 151 +/- 93 torr (20.0 +/- 12.4 kPa) and continued to improve throughout the study (p<.05). Animals that received volume controlled positive pressure breathing remained hypoxic with no appreciable change in PaO2. Although both groups developed hypercarbia during oleic acid infusion, PaCO2, steadily increased over time in the control group (p<.01). Static lung compliance significantly increased postrandomization (60 mins) in the animals supported by perflurocarbon-associated gas exchange (p<.05), whereas it remained unchanged over time in the volume controlled positive pressure breathing group. However, survival was significantly higher in the perfluorocarbon-associated gas exchange group with eight (80%) of ten animals surviving the entire study period. Only two (25%) of the eight animals in the volume controlled positive pressure breathing group were alive at the end of the study period (log-rank statistic, p=.013).
  
  
  CONCLUSIONS
  Perflurocarbon-associated gas exchange enhanced gas exchange, pulmonary mechanics, and survival in this model of acute lung injury.
authors.affiliation: Division of Pediatric Critical Care Medicine, Children's Hospital of Buffalo (SUNY), New York 14222, USA.
authors.forename: M C
authors.initials: MC
authors.lastname: Papo
authors.forename: P R
authors.initials: PR
authors.lastname: Paczan
authors.forename: B P
authors.initials: BP
authors.lastname: Fuhrman
authors.forename: D M
authors.initials: DM
authors.lastname: Steinhorn
authors.forename: L J
authors.initials: LJ
authors.lastname: Hernan
authors.forename: C L
authors.initials: CL
authors.lastname: Leach
authors.forename: B A
authors.initials: BA
authors.lastname: Holm
authors.forename: J E
authors.initials: JE
authors.lastname: Fisher
authors.forename: B A
authors.initials: BA
authors.lastname: Kahn
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D006801:Humans; D010100:Oxygen / Q000097:blood*; D011175:Positive-Pressure Respiration / Q000379:methods; D011446:Prospective Studies; D011897:Random Allocation; D012121:Respiration, Artificial; D012128:Respiratory Distress Syndrome / Q000401:mortality / Q000503:physiopathology / Q000628:therapy*; D015656:Respiratory Mechanics / Q000187:drug effects*; D016019:Survival Analysis; D013552:Swine
chemical_list: D010100:Oxygen