title: Effects of microgravity on osteoblast growth activation.
abstract: Space flight is an environmental condition where astronauts can lose up to 19% of weight-bearing bone during long duration missions. We used the MC3T3-E1 osteoblast to investigate bone cell growth in microgravity (10(-6) to 10(-9)g). Osteoblasts were launched on the STS-56 shuttle flight in a quiescent state with 0.5% fetal calf serum (FCS) medium and growth activation was initiated by adding fresh medium with 10% FCS during microgravity exposure. Four days after serum activation, the cells were fixed before return to normal Earth gravity. Ground controls were treated in parallel with the flight samples in identical equipment. On landing, cell number, cell cytoskeleton, glucose utilization, and prostaglandin synthesis in flight (n = 4) and ground controls (n = 4) were examined. The flown osteoblasts grew slowly in microgravity with total cell number significantly reduced (55 +/- 6 vs 141 +/- 8 cells per microscopic field). The cytoskeleton of the flight osteoblasts had a reduced number of stress fibers and a unique abnormal morphology. Nuclei in the ground controls were large and round with punctate Hoechst staining of the DNA nucleosomes. The flight nuclei were 30% smaller than the controls (P < 0.0001) and oblong in shape, with fewer punctate areas. Due to their reduced numbers, the cells activated in microgravity used significantly less glucose than ground controls (80.2 +/- 0.7 vs 50.3 +/- 3.7 mg of glucose/dl remaining in the medium) and had reduced prostaglandin E2 (PGE2) synthesis when compared to controls (57.3 +/- 17 vs 138.3 +/- 41 pmol/ml). Cell viability was normal since, on a per-cell basis, glucose use and prostaglandin synthesis were comparable for flight and ground samples. Taken together, these data suggest that growth activation in microgravity results in reduced growth, causing reduced glucose utilization and reduced prostaglandin synthesis, with significantly altered actin cytoskeleton in osteoblasts.
authors.affiliation: Laboratory of Cell Growth and Differentiation, Department of Veterans Affairs, San Francisco, California, 94121, USA.
authors.forename: M
authors.initials: M
authors.lastname: Hughes-Fulford
authors.forename: M L
authors.initials: ML
authors.lastname: Lewis
mesh_terms: D000818:Animals; D002455:Cell Division; D002467:Cell Nucleus / Q000648:ultrastructure; D003470:Culture Media; D003599:Cytoskeleton / Q000648:ultrastructure; D051379:Mice; D010006:Osteoblasts / Q000502:physiology*; D011453:Prostaglandins / Q000096:biosynthesis; D013026:Space Flight; D014893:Weightlessness
chemical_list: D003470:Culture Media; D011453:Prostaglandins