High-level secretion of a virally encoded anti-fungal toxin in transgenic tobacco plants.
Ustilago maydis killer toxins are small polypeptides (7-14 kDa) which kill susceptible cells of closely related fungal species. The KP4 toxin is a single polypeptide subunit with a molecular weight of 11.1 kDa. In this work, a transgenic tobacco plant was constructed which secretes the KP4 toxin at a high level. The KP4 toxin expressed in this transgenic plant was of the same size and specificity as the authentic Ustilago KP4 toxin. The expression level was at least 500 times higher than that of the KP6 toxin expressed in plants. Transgenic crop plants producing the KP4 toxin could be rendered resistant to KP4-susceptible fungal pathogens.
Park
C M
CM
Department of Biological Sciences, State University of New York at Buffalo, 14260, USA.
Berry
J O
JO
Bruenn
J A
JA
D015336:Molecular Probe Techniques; D009183:Mycotoxins / Q000235:genetics / Q000378:metabolism*; D010935:Plant Diseases; D030821:Plants, Genetically Modified; D012328:RNA Viruses / Q000235:genetics*; D011994:Recombinant Proteins / Q000378:metabolism; D014588:Ustilago / Q000821:virology*; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
D009183:Mycotoxins; D011994:Recombinant Proteins; D014764:Viral Proteins; C086592:Ustilago maydis viral toxin KP4