title: Microsatellite instability in follicle centre cell lymphoma.
abstract: Fluorescent polymerase chain reaction (PCR) was used to assay 12 microsatellite markers (APC x 2, DCC, P53 x 2, RB1, NM23, WT1, D6S260, D6S262, D6S281 and TNFa) to look for evidence of microsatellite instability in 40 cases of follicle centre cell lymphoma (FCC). Evidence of novel alleles seen in the tumour tissue but not the normal uninvolved tissue was seen in seven cases (17%). In only two of these cases (5%) was more than one locus involved but in these cases multiple affected loci were seen (4/12 and 7/12 respectively). The detection of microsatellite instability indicates a DNA repair defect such as that which would be predicted to occur in cells with mutated mismatch repair genes, a novel finding in FCC lymphoma.
authors.affiliation: Centre for Haematologic Oncology, University of Leeds.
authors.forename: J
authors.initials: J
authors.lastname: Randerson
authors.forename: L
authors.initials: L
authors.lastname: Cawkwell
authors.forename: A
authors.initials: A
authors.lastname: Jack
authors.forename: J A
authors.initials: JA
authors.lastname: Child
authors.forename: F
authors.initials: F
authors.lastname: Lewis
authors.forename: N
authors.initials: N
authors.lastname: Hall
authors.forename: P
authors.initials: P
authors.lastname: Johnson
authors.forename: P
authors.initials: P
authors.lastname: Evans
authors.forename: S
authors.initials: S
authors.lastname: Barrans
authors.forename: G J
authors.initials: GJ
authors.lastname: Morgan
mesh_terms: D002883:Chromosomes, Human, Pair 14; D002887:Chromosomes, Human, Pair 18; D004260:DNA Repair; D006801:Humans; D008224:Lymphoma, Follicular / Q000235:genetics*; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction; D011518:Proto-Oncogene Proteins / Q000235:genetics; D019253:Proto-Oncogene Proteins c-bcl-2; D014178:Translocation, Genetic
chemical_list: D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2