Immunohistochemical analysis of statin in colorectal adenocarcinoma, polyps, and normal mucosa.
OBJECTIVE
The search for an understanding of the kinetics of the malignant cell is an ongoing focus of research. The aim of the present study was to determine whether there were any differences in the expression of statin, a nonproliferation-specific nuclear protein, among different colorectal tissues and whether there is any relationship between statin presence and neoplastic aggressivity.


METHODS
The study population consisted of specimens from 19 patients who underwent resection for carcinoma, one for villous adenoma, and seven for colonoscopic polypectomies. Tissue samples were taken from the center of the specimen and from mucosa 10 cm from the lesion.


RESULTS
Statin immunoreactivity was evaluated by counting stained nuclei in ten randomly chosen fields, and percent of positive cells was calculated. Average percentage of statin-positive cells was 34.33 +/- 6.81 in the normal crypt, 44.42 +/- 7.28 for polyps, and 7.74 +/- 5.67 for carcinomas (significantly lower than normal mucosa and polyps; P < 0.001).


CONCLUSIONS
Statin expression is dramatically diminished in invasive carcinoma tissue, but it did not help determine aggressivity with respect to Dukes stage.
Kyzer
S D
SD
Department of Surgery, Lady Davis Institute for Medical Research, Montreal, Quebec, Canada.
Gordon
P H
PH
Wang
E
E
D000230:Adenocarcinoma / Q000737:chemistry* / Q000473:pathology; D000368:Aged; D000911:Antibodies, Monoclonal; D018797:Cell Cycle Proteins; D002455:Cell Division; D003106:Colon / Q000378:metabolism* / Q000473:pathology; D003111:Colonic Polyps / Q000378:metabolism* / Q000473:pathology; D015179:Colorectal Neoplasms / Q000737:chemistry* / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D020648:Peptide Elongation Factor 1; D011506:Proteins / Q000032:analysis*
D000911:Antibodies, Monoclonal; D018797:Cell Cycle Proteins; C503234:EEF1A2 protein, human; D020648:Peptide Elongation Factor 1; D011506:Proteins