The chsA gene, encoding a class-I chitin synthase from Ampelomyces quisqualis.
Degenerate oligodeoxyribonucleotide primers, designed on the basis of conserved regions of the chitin synthase gene family, were used to amplify a fragment of the Ampelomyces quisqualis (Aq) chsA gene. Subsequently, the PCR product was used as a probe in order to identify and isolate genomic clones harboring the entire chsA gene. Aq chsA is 2786-nt long, has one intron and encodes a 910-amino-acid polypeptide belonging to the class-I chitin synthases. Low-stringency Southern hybridizations to Aq genomic DNA provided evidence for the presence of additional DNA fragments resembling chsA in the fungal genome, suggesting the presence of a multigene family of chitin synthases in Aq.
Weiss
N
N
Department of Plant Pathology and Microbiology, Faculty of Agriculture, Hebrew University of Jerusalem, Israel.
Sztejnberg
A
A
Yarden
O
O
D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D002686:Chitin / Q000235:genetics; D002687:Chitin Synthase / Q000737:chemistry / Q000235:genetics*; D003001:Cloning, Molecular; D017931:DNA Primers / Q000737:chemistry; D005800:Genes, Fungal; D003904:Mitosporic Fungi / Q000201:enzymology / Q000235:genetics*; D008969:Molecular Sequence Data; D005810:Multigene Family; D017421:Sequence Analysis
D017931:DNA Primers; D002686:Chitin; D002687:Chitin Synthase