High-performance liquid chromatographic determination of the silicon phthalocyanine Pc 4 in human blood.
A sensitive and reproducible method has been developed for the measurement of the silicon phthalocyanine Pc 4 in red blood cell concentrates (RBCC). The procedure involves extraction of the RBCC with acetonitrile, purification of the extracts with reversed-phase Sep-Pak C18 cartridges and determination of Pc 4 in the extracts by high-performance liquid chromatography (HPLC) using a reversed-phase C18 column. The detection limit with 1-ml RBCC samples is 2 ng. This method is applicable to monitoring Pc 4 during its use as a photosensitizer for the inactivation of viruses in RBCC prior to transfusion. It has the potential to be adapted for measuring Pc 4 in tissues during its use in photodynamic therapy of cancer.
Zuk
M M
MM
New York Blood Center, NY 10021, USA.
Kenney
M E
ME
Horowitz
B
B
Ben-Hur
E
E
D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000706:statistics & numerical data; D006801:Humans; D007211:Indoles / Q000097:blood*; D017646:Organosilicon Compounds / Q000097:blood*; D017319:Photosensitizing Agents / Q000097:blood*; D012680:Sensitivity and Specificity; D012821:Silanes
D007211:Indoles; D017646:Organosilicon Compounds; D017319:Photosensitizing Agents; D012821:Silanes; C082854:silicon phthalocyanine; C052464:bis(tri-n-hexylsiloxy)(2,3-naphthalocyaninato)silicon