Hepatitis E virus (87A strain) propagated in A549 cells.
A strain of hepatitis E virus (HEV), the 87A strain isolated in 2BS cells from the feces of a patient with hepatitis E, has been reported previously. In this study, the 87A strain was propagated in A549 cells, and the marked cytopathic effect (CPE) appeared in the infected monolayer cells. The size of this virus is about 30 nm in diameter. Furthermore, HEV-RNA from the supernatants of the virus of different passages was detected by polymerase chain reaction (PCR) amplification using ET1.1 HEV primers. A band of HEV for 239 bp from PCR products was revealed by electrophoresis. PCR products of the fourth passage were sequenced. These results show that the 87A virus replicates in the A549 cell line.
Huang
R
R
Department of Public Health, Kansai Medical University, Osaka, Japan.
Nakazono
N
N
Ishii
K
K
Li
D
D
Kawamata
O
O
Kawaguchi
R
R
Tsukada
Y
Y
D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D006224:Cricetinae; D016751:Hepatitis E / Q000821:virology; D016752:Hepatitis E virus / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification; D006801:Humans; D016133:Polymerase Chain Reaction; D014709:Vero Cells