Expression of cytokeratin 8 in basal cell carcinoma: a comparative immunohistochemical and immunoelectron microscopy study.
The comparative study reported here was undertaken in order to resolve the discrepancies in the detection of cytokeratin (Ck) 8 reported in previous studies. The expression of Ck 8 was compared in 6 basal cell carcinomas (BCCs) using immunohistochemical and immunoelectron microscopic techniques and a panel of 4 different commercially available monoclonal antibodies (MoAbs). The results of this comparative study demonstrated not only that the consistent expression of Ck 8 using one of the MoAbs in immunohistochemistry was confirmed by immunoelectron microscopy, but that the inconsistent expression of Ck 8 observed using two other MoAbs was also confirmed. One of the MoAbs did not show any staining at all. The inability of this MoAb to detect the expression of Ck 8 using either of the techniques also indicated that this MoAb may be directed against an epitope of Ck8 that is not detectable in BCC in situ.
Kooy
A J
AJ
Department of Dermato-Venereology, Erasmus University, Rotterdam, The Netherlands.
Tank
B
B
de Jong
T A
TA
Vuzevski
V D
VD
Bosman
F T
FT
van Joost
T
T
D000368:Aged; D000911:Antibodies, Monoclonal; D002280:Basal Cell Carcinoma / Q000737:chemistry* / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007633:Keratins / Q000032:analysis*; D008297:Male; D016253:Microscopy, Immunoelectron; D008875:Middle Aged; D012878:Skin Neoplasms / Q000737:chemistry* / Q000473:pathology
D000911:Antibodies, Monoclonal; D007633:Keratins