title: Mechanical effects on the kinetics of the HIV proteinase deactivation.
abstract: The proteinase from HIV undergoes rapid and irreversible deactivation caused by mild mechanical stirring. Both the free enzyme and the ternary Michaelis complex disappear in two separate first-order processes, with half-times of 3.0 and 0.8 minutes, respectively. Ignoring these deactivation steps distorts the results of kinetic analyses.
authors.affiliation: School of Pharmacy, Department of Chemistry, University of Wisconsin, Madison 53706, USA.
authors.forename: P
authors.initials: P
authors.lastname: Kuzmic
authors.forename: A G
authors.initials: AG
authors.lastname: Peranteau
authors.forename: G
authors.initials: G
authors.lastname: García-Echeverría
authors.forename: D H
authors.initials: DH
authors.lastname: Rich
mesh_terms: D000595:Amino Acid Sequence; D002384:Catalysis; D016333:HIV Protease / Q000378:metabolism*; D017320:HIV Protease Inhibitors / Q000494:pharmacology*; D006868:Hydrolysis; D007700:Kinetics; D008969:Molecular Sequence Data; D013379:Substrate Specificity
chemical_list: D017320:HIV Protease Inhibitors; D016333:HIV Protease