Retention behaviour of anti-emetic serotonin antagonists in reversed phase high performance liquid chromatography.
Granisetron, Ondansetron and Tropisetron, three 5-HT3 antagonists showing anti-emetic activity, were analysed by HPLC on lipophilic stationary phases. The addition of an amine or quaternary ammonium salt to the eluents was a powerful tool in the analysis of these basic substances. The influence on chromatographic parameters of pH, ionic strength and various counter-ions in the aqueous phase as well as of different organic modifiers is discussed. Some of the proposed experimental conditions allow a more strictly partition-based separation mechanism and can produce chromatographic parameters suitable for structure-activity studies. These experimental conditions are also suitable for analysis of the considered compounds in pharmaceutical dosage forms or in biological fluids.
Barbato
F
F
Dipartimento di Chimica Farmaceutica e Tossicologica, Facoltà di Farmacia Università degli Studi di Napoli Federico II, Italy.
La Rotonda
M I
MI
Quaglia
F
F
D000932:Antiemetics / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D017829:Granisetron / Q000032:analysis*; D007211:Indoles / Q000032:analysis*; D017294:Ondansetron / Q000032:analysis*; D012702:Serotonin Antagonists / Q000032:analysis*; D000077526:Tropisetron
D000932:Antiemetics; D017829:Granisetron; D007211:Indoles; D017294:Ondansetron; D012702:Serotonin Antagonists; D000077526:Tropisetron