[Effect of free radicals on proliferation and metabolism of chondrocytes and on sensitivity of a cellular monolayer to proteases].
Oxygen radicals (OR) were demonstrated to suppress the proliferation of chondrocytes and to decrease the synthesis of collagen by these cells. The concomitant action of OR and proteases (collagenase or trypsin) sharply increased the detachment of cells and destruction of the intercellular matrix at very low enzymatic concentrations (10-100-fold dilution of routine doses for cultural techniques) as compared with the control.
Panasiuk
A F
AF
Iaroshuk
G V
GV
Mitrovich
D
D
D000818:Animals; D002358:Cartilage, Articular / Q000166:cytology / Q000378:metabolism*; D002455:Cell Division; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis; D017364:Collagenases / Q000378:metabolism*; D005609:Free Radicals; D006868:Hydrolysis; D011817:Rabbits; D014357:Trypsin / Q000378:metabolism*
D005609:Free Radicals; D003094:Collagen; D014357:Trypsin; D017364:Collagenases