title: The crystal structure of the DNA-binding domain of yeast RAP1 in complex with telomeric DNA.
abstract: Telomeres, the nucleoprotein complexes at the ends of eukaryotic chromosomes, are essential for chromosome stability. In the yeast S. cerevisiae, telomeric DNA is bound in a sequence-specific manner by RAP1, a multifunctional protein also involved in transcriptional regulation. Here we report the crystal structure of the DNA-binding domain of RAP1 in complex with telomeric DNA site at 2.25 A resolution. The protein contains two similar domains that bind DNA in a tandem orientation, recognizing a tandemly repeated DNA sequence. The domains are structurally related to the homeodomain and the proto-oncogene Myb, but show novel features in their DNA-binding mode. A structured linker between the domains and a long C-terminal tail contribute to the binding specificity. This structure provides insight into the recognition of the conserved telomeric DNA sequences by a protein.
authors.affiliation: Medical Research Council, Laboratory of Molecular Biology, Cambridge, United Kingdom.
authors.forename: P
authors.initials: P
authors.lastname: Konig
authors.forename: R
authors.initials: R
authors.lastname: Giraldo
authors.forename: L
authors.initials: L
authors.lastname: Chapman
authors.forename: D
authors.initials: D
authors.lastname: Rhodes
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003461:Crystallography; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism; D005656:Fungal Proteins / Q000737:chemistry*; D019204:GTP-Binding Proteins / Q000737:chemistry* / Q000378:metabolism; D007091:Image Processing, Computer-Assisted; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D016615:Telomere / Q000737:chemistry* / Q000378:metabolism; D015003:Yeasts / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D020668:rap GTP-Binding Proteins
chemical_list: D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D019204:GTP-Binding Proteins; D020668:rap GTP-Binding Proteins