Asymmetric accumulation of Ash1p in postanaphase nuclei depends on a myosin and restricts yeast mating-type switching to mother cells.
Cell division in haploid yeast gives rise to a "mother" cell capable of mating-type switching and a "daughter" cell that is not. Switching is initiated by the HO endonuclease, whose gene is only transcribed in cells that have previously given birth to a bud (mother cells). HO expression depends on a minimyosin, She1p/Myo4p, which accumulates preferentially in growing buds. We describe a gene, ASH1, that is necessary to repress HO in daughters. ASH1 encodes a zinc finger protein whose preferential accumulation in daughter cell nuclei at the end of anaphase depends on She1p/Myo4p. The greater abundance of Ash1p in daughter cells is responsible for restricting HO expression to mother cells.
Bobola
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Research Institute of Molecular Pathology, Vienna, Austria.
Jansen
R P
RP
Shin
T H
TH
Nasmyth
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K
D000595:Amino Acid Sequence; D000705:Anaphase; D001483:Base Sequence; D002467:Cell Nucleus / Q000378:metabolism*; D003001:Cloning, Molecular; D003433:Crosses, Genetic; D004268:DNA-Binding Proteins; D015252:Deoxyribonucleases, Type II Site-Specific / Q000096:biosynthesis* / Q000235:genetics; D000939:Epitopes / Q000032:analysis; D005656:Fungal Proteins / Q000096:biosynthesis / Q000378:metabolism*; D015870:Gene Expression; D005800:Genes, Fungal; D049770:Genes, Mating Type, Fungal; D008969:Molecular Sequence Data; D016296:Mutagenesis; D018995:Myosin Heavy Chains; D024701:Myosin Type V; D009218:Myosins / Q000096:biosynthesis / Q000378:metabolism*; D016366:Open Reading Frames; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry; D012097:Repressor Proteins; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000235:genetics / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D014157:Transcription Factors / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D014158:Transcription, Genetic; D016335:Zinc Fingers
C098733:ASH1 protein, S cerevisiae; D004268:DNA-Binding Proteins; D000939:Epitopes; D005656:Fungal Proteins; C098732:Myo4 protein, S cerevisiae; D011994:Recombinant Proteins; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; C530845:HO protein, S cerevisiae; C037725:SCEI protein, S cerevisiae; D015252:Deoxyribonucleases, Type II Site-Specific; D024701:Myosin Type V; D018995:Myosin Heavy Chains; D009218:Myosins