Molecular cloning and characterization of a gibberellin-inducible, putative alpha-glucosidase gene from barley.
A putative alpha-glucosidase clone has been isolated from a cDNA library constructed from mRNA of barley aleurones treated with gibberellin A 3 (GA). The clone is 2752 bp in length and has an uninterrupted open reading frame encoding a polypeptide of 877 amino acids. A 680 amino acid region is 43% identical to human lysosomal alpha-glucosidase and other glycosyl hydrolases. In isolated aleurones, the levels of the corresponding mRNA increase strongly after the application of GA, similar to the pattern exhibited by low-pI alpha-amylase mRNA. High levels are also observed in the aleurone and scutellum after germination, while low levels are found in developing seeds. The genome contains a single form of this alpha-glucosidase gene and two additional sequences that may be related genes or pseudogenes.
Tibbot
B K
BK
Department of Agronomy, University of Wisconsin, Madison, WI 53706, USA.
Skadsen
R W
RW
D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D017124:Conserved Sequence; D018076:DNA, Complementary / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D015723:Gene Library; D018745:Genome, Plant; D005875:Gibberellins / Q000494:pharmacology*; D001467:Hordeum / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D014018:Tissue Distribution; D000520:alpha-Glucosidases / Q000096:biosynthesis / Q000235:genetics*
D018076:DNA, Complementary; D005875:Gibberellins; D000520:alpha-Glucosidases