Expression of endothelin precursor genes in human trophoblast in culture.
We have shown previously the presence of immunoreactive endothelin in cultured trophoblastic cells from human term placenta as well as in the trophoblast-conditioned medium. To confirm whether or not the differentiated syncytiotrophoblast is a site for endothelin synthesis, we investigated, by reverse transcription and polymerase chain reaction, the expression of the three preproendothelin genes in 3-day cultured trophoblast. While no endothelin-2 precursor mRNA was detected, preproendothelin-1 mRNA was found to be expressed by the trophoblast. The endothelin-3 precursor gene was also expressed, but at low level and it was detected only after Southern blotting and oligonucleotide hybridization. The ability of trophoblast in culture to express the endothelin precursor genes supports the idea that, in human term placenta, villous syncytiotrophoblast that lines the intervillous space containing maternal blood acts as an endothelial layer.
Robert
B
B
INSERM U.361, Université René Descartes, Maternité Baudelocque, Paris, France.
Malassiné
A
A
Bourgeois
C
C
Mignot
T M
TM
Cronier
L
L
Ferré
F
F
Duc-Goiran
P
P
D001483:Base Sequence; D015139:Blotting, Southern; D002478:Cells, Cultured; D019332:Endothelin-1; D016232:Endothelins / Q000235:genetics*; D015870:Gene Expression; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011498:Protein Precursors / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism; D014327:Trophoblasts / Q000378:metabolism*; D001613:beta 2-Microglobulin / Q000235:genetics
D019332:Endothelin-1; D016232:Endothelins; D011498:Protein Precursors; D012333:RNA, Messenger; D001613:beta 2-Microglobulin; C099648:preproendothelin 2; C075625:preproendothelin-3