Characterisation of antimitotic products from marine organisms that disorganise the microtubule network: ecteinascidin 743, isohomohalichondrin-B and LL-15.
The effect of selected marine compounds with anti-tumoral activity on the cell microtubule network was tested by immunofluorescence analyses, or by other in vitro analyses involving competition with colchicine or with GTP for tubulin binding and tubulin polymerisation, studies that were carried out in parallel with other microtubule poisons used as controls. Three compounds were found to disorganise the microtubule network: isohomohalichondrin B, LL-15 and ecsteinascidin 743. The first two compounds prevent microtubule assembly and GTP binding to tubulin. Ecteinascidin 743 disorganises the microtubule network but it does not seem to interact directly with tubulin.
García-Rocha
M
M
Centro de Biología Molecular Severo Ochoa, Universidad Autónoma de Madrid, Spain.
García-Gravalos
M D
MD
Avila
J
J
D008841:Actin Cytoskeleton / Q000187:drug effects; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D004149:Dioxoles / Q000494:pharmacology*; D006160:Guanosine Triphosphate / Q000378:metabolism; D006367:HeLa Cells / Q000187:drug effects; D006801:Humans; D007546:Isoquinolines / Q000494:pharmacology*; D051379:Mice; D008870:Microtubules / Q000187:drug effects*; D009281:Naphthalenes / Q000494:pharmacology*; D011714:Pyrans / Q000494:pharmacology*; D013141:Spiro Compounds / Q000494:pharmacology*; D044005:Tetrahydroisoquinolines; D000077606:Trabectedin
D000970:Antineoplastic Agents; D004149:Dioxoles; D007546:Isoquinolines; C099149:LL 15; D009281:Naphthalenes; D011714:Pyrans; D013141:Spiro Compounds; D044005:Tetrahydroisoquinolines; C099151:isohomohalichondrin B; D006160:Guanosine Triphosphate; D000077606:Trabectedin