title: Coronavirus isolation from nasal swab samples in cattle with signs of respiratory tract disease after shipping.
abstract: OBJECTIVE
  To monitor the prevailing viral respiratory tract infections in cattle after transportation to feedlots.
  
  
  METHODS
  100 cattle with signs of respiratory tract disease on arrival at 2 feedlots.
  
  
  METHODS
  Nasal swab samples were obtained from each animal and were used for inoculation of defined cell culture systems that detected bovine viruses known to cause respiratory tract infections, as well as viruses previously not recognized as respiratory pathogens for cattle.
  
  
  RESULTS
  Bovine respiratory coronaviruses were isolated from 38 of the 100 cattle, including 6 of 50 cattle from California, 22 of 31 cattle from Oklahoma, 6 of 11 cattle from Texas, and 4 of 8 cattle of unknown origin. Parainfluenza 3 viruses also were isolated from 5 California cattle, but other bovine viruses were not detected.
  
  
  CONCLUSIONS
  The high rate of coronavirus isolations from feedlot cattle with signs of respiratory tract disease implied wide distribution and high susceptibility among cattle to this infection, which had not been detected by use of viral isolation systems in previous etiologic evaluations of feedlot cattle affected with bovine respiratory disease complex.
authors.affiliation: Department of Veterinary Microbiology and Parasitology, School of Veterinary Medicine, Louisiana State University, Baton Rouge 70803, USA.
authors.forename: J
authors.initials: J
authors.lastname: Storz
authors.forename: L
authors.initials: L
authors.lastname: Stine
authors.forename: A
authors.initials: A
authors.lastname: Liem
authors.forename: G A
authors.initials: GA
authors.lastname: Anderson
mesh_terms: D000818:Animals; D001130:Arizona / Q000453:epidemiology; D002140:California / Q000453:epidemiology; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology / Q000821:virology*; D002460:Cell Line; D017934:Coronavirus / Q000302:isolation & purification*; D018352:Coronavirus Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D007615:Kansas / Q000453:epidemiology; D009297:Nasal Mucosa / Q000821:virology*; D009825:Oklahoma / Q000453:epidemiology; D015995:Prevalence; D012141:Respiratory Tract Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D013781:Texas / Q000453:epidemiology; D014186:Transportation