Peptide epitopes from noncytosolic Listeria monocytogenes can be presented by major histocompatibility complex class I molecules.
Listeria monocytogenes is an intracellular pathogen which escapes the phagosome and resides in the cytosol of the host cell. Using Listeria innocua and a mutant strain of L. monocytogenes (listeriolysin O negative), which do not enter the cytosol of the host cell, we demonstrate class I presentation of an epitope of p60, a protein secreted by L. monocytogenes, to a class I-restricted CD8+ cytotoxic T lymphocyte clone.
Zwickey
H L
HL
Department of Medicine, National Jewish Center for Immunology and Respiratory Medicine, Denver, CO 80206-2761, USA.
Potter
T A
TA
D000818:Animals; D017951:Antigen Presentation; D000938:Antigen-Presenting Cells / Q000276:immunology / Q000382:microbiology; D000942:Antigens, Bacterial / Q000378:metabolism*; D001427:Bacterial Toxins; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D002460:Cell Line; D003600:Cytosol / Q000382:microbiology; D003602:Cytotoxicity, Immunologic; D000939:Epitopes / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000235:genetics / Q000276:immunology; D006460:Hemolysin Proteins; D015395:Histocompatibility Antigens Class I / Q000378:metabolism*; D066298:In Vitro Techniques; D008089:Listeria monocytogenes / Q000235:genetics / Q000276:immunology*; D008264:Macrophages / Q000276:immunology / Q000382:microbiology; D051379:Mice; D008807:Mice, Inbred BALB C; D009154:Mutation; D010455:Peptides / Q000276:immunology* / Q000378:metabolism
D000942:Antigens, Bacterial; D001427:Bacterial Toxins; D000939:Epitopes; D006360:Heat-Shock Proteins; D006460:Hemolysin Proteins; D015395:Histocompatibility Antigens Class I; D010455:Peptides; C038705:hlyA protein, Listeria monocytogenes