Localization of the human homolog of the yeast cell division control 27 gene (CDC27) proximal to ITGB3 on human chromosome 17q21.3.
The human homolog of the Saccharomyces cerevisiae cell division control 27 gene (CDC27) was mapped to human chromosome 17q12-q21 using a panel of human/rodent somatic cell hybrids and localized distal to the breast cancer susceptibility gene, BRCA1, using a panel of radiation hybrids. The radiation hybrid panel indicates that the most likely position of human CDC27 on human chromosomes 17 is between the marker D17S409 and the beta 3 subunit of integrin (ITGB3). Further confirmation of this localization comes from the sequence tagged site (STS) mapping of human CDC27 to the same yeast artificial chromosomes (YACs) positive for ITGB3. The estimated distance between ITGB3 and human CDC27 is less than 600 kb.
Ho
P P
PP
Wayne State University, Department of Immunology and Microbiology, Detroit, Michigan 48201, USA.
Couch
F J
FJ
Brody
L C
LC
Abel
K J
KJ
Boehnke
M
M
Shearon
T H
TH
Chandrasekharappa
S C
SC
Collins
F S
FS
Weber
B L
BL
D000818:Animals; D015703:Antigens, CD / Q000235:genetics*; D064191:Apc3 Subunit, Anaphase-Promoting Complex-Cyclosome; D019313:BRCA1 Protein; D001483:Base Sequence; D001943:Breast Neoplasms / Q000235:genetics; D018797:Cell Cycle Proteins / Q000235:genetics*; D002455:Cell Division / Q000235:genetics; D002874:Chromosome Mapping; D018244:Chromosomes, Artificial, Yeast; D002886:Chromosomes, Human, Pair 17; D006224:Cricetinae; D004258:DNA Polymerase III; D017931:DNA Primers; D005260:Female; D005819:Genetic Markers; D006801:Humans; D006822:Hybrid Cells; D039661:Integrin beta3; D051379:Mice; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000235:genetics*; D016366:Open Reading Frames; D010980:Platelet Membrane Glycoproteins / Q000235:genetics*; D016133:Polymerase Chain Reaction; D051381:Rats; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D012568:Schizosaccharomyces / Q000166:cytology / Q000235:genetics; D029702:Schizosaccharomyces pombe Proteins; D016324:Sequence Tagged Sites; D014157:Transcription Factors / Q000235:genetics*; D044767:Ubiquitin-Protein Ligases
D015703:Antigens, CD; D064191:Apc3 Subunit, Anaphase-Promoting Complex-Cyclosome; D019313:BRCA1 Protein; C577355:CDC27 protein, S cerevisiae; C577366:CDC27 protein, human; D018797:Cell Cycle Proteins; D017931:DNA Primers; D005819:Genetic Markers; D039661:Integrin beta3; D009363:Neoplasm Proteins; D010980:Platelet Membrane Glycoproteins; D029701:Saccharomyces cerevisiae Proteins; D029702:Schizosaccharomyces pombe Proteins; D014157:Transcription Factors; C513024:cdc27 protein, S pombe; D044767:Ubiquitin-Protein Ligases; D004258:DNA Polymerase III