Positive elements in the laminin gamma 1 gene synergize to activate high level transcription during cellular differentiation.
Transcription of the murine laminin gamma 1 gene is activated during retinoic acid/cAMP induced differentiation of F9 embryonal carcinoma cells. Positive transcription control elements associated with two DNase I hypersensitive regions in the large first intron of the gene have been identified which confer a differentiation response on the laminin gamma 1 promoter. However, the kinetics of transcriptional activation suggest each DNA region interacts with transcription factors appearing at different times during differentiation. Synergy between the two regions in cis causes high level activation.
Chang
H S
HS
Department of Molecular Genetics and Biochemistry, University of Pittsburgh School of Medicine, PA 15261, USA.
Kim
N B
NB
Phillips
S L
SL
D000818:Animals; D001483:Base Sequence; D017931:DNA Primers / Q000737:chemistry; D003850:Deoxyribonuclease I; D004742:Enhancer Elements, Genetic; D018507:Gene Expression Regulation, Developmental; D007438:Introns; D007797:Laminin / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics; D015183:Restriction Mapping; D017384:Sequence Deletion; D014158:Transcription, Genetic
D017931:DNA Primers; D007797:Laminin; D012333:RNA, Messenger; C089141:laminin gamma 1; D003850:Deoxyribonuclease I