Cloning and sequencing of a cDNA encoding a larval-pupal-specific cuticular protein in Tenebrio molitor (Insecta, Coleoptera). Developmental expression and effect of a juvenile hormone analogue.
A cDNA clone encoding a larval-pupal cuticular protein, named TMLPCP-22, has been isolated by screening a library in expression vector with a monoclonal antibody made against pupal cuticular proteins of Tenebrio molitor. Northern-blot and in situ hybridization analyses showed that the expression of TMLPCP-22 is regulated in a stage-specific and tissue-specific manner; the transcript was present during the secretion of preecdysial larval and pupal cuticles and was restricted to epidermal cells. No expression was observed during adult cuticle deposition. In supernumerary pupae obtained after application of a juvenile hormone analogue, which is known to inhibit the adult programme, TMLPCP-22 mRNA was expressed again, confirming its larval-pupal specificity.
Rondot
I
I
CNRS URA 674, Université de Bourgogne, Dijon, France.
Quennedey
B
B
Courrent
A
A
Lemoine
A
A
Delachambre
J
J
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D017344:Genes, Insect; D017403:In Situ Hybridization; D007605:Juvenile Hormones / Q000494:pharmacology; D007814:Larva / Q000235:genetics; D008675:Metamorphosis, Biological / Q000235:genetics; D008969:Molecular Sequence Data; D011506:Proteins / Q000235:genetics*; D011679:Pupa / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013712:Tenebrio / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development
D017931:DNA Primers; D018076:DNA, Complementary; D007605:Juvenile Hormones; D011506:Proteins; D012333:RNA, Messenger