title: Studies into the microbial spectrum of apical periodontitis.
abstract: This study examined the variety of obligate and facultative anaerobic bacterial species recovered from cases of acute apical periodontitis. A total of 19 root canal samples and 24 periapical granuloma samples were taken from patients suffering pain and discomfort. Bacteria were identified by applying the following techniques: culturing on various media, Gram-staining and using commercially available biochemical test strips. In addition, Prevotella intermedia and Porphyromonas endodontalis were differentiated on a molecular genetic level using species-specific oligodeoxynucleotide probes. The most frequently identified bacteria were Prevotella intermedia, Bifidobacterium spp., Streptococcus sanguis, Streptococcus milleri-group and Bacteroides spp. Obligate anaerobes occurred at a rate of 82.3%, and the average number of isolates was 6.4 per sample.
authors.affiliation: Clinic of Conservative and Preventive Dentistry & Periodontology, Technical University (RWTH), Aachen, Germany.
authors.forename: A W
authors.initials: AW
authors.lastname: Brauner
authors.forename: G
authors.initials: G
authors.lastname: Conrads
mesh_terms: D000190:Actinomyces / Q000302:isolation & purification; D000293:Adolescent; D000328:Adult; D015373:Bacterial Typing Techniques; D001439:Bacteroides / Q000302:isolation & purification; D001644:Bifidobacterium / Q000302:isolation & purification; D002206:Capnocytophaga / Q000302:isolation & purification; D002648:Child; D015342:DNA Probes; D004269:DNA, Bacterial / Q000032:analysis; D003786:Dental Pulp Cavity / Q000382:microbiology; D005051:Eubacterium / Q000302:isolation & purification; D005260:Female; D005673:Fusobacterium / Q000302:isolation & purification; D006089:Gram-Negative Anaerobic Bacteria / Q000302:isolation & purification; D006801:Humans; D008297:Male; D008875:Middle Aged; D010484:Periapical Granuloma / Q000382:microbiology*; D010485:Periapical Periodontitis / Q000382:microbiology; D017861:Porphyromonas / Q000302:isolation & purification; D018720:Prevotella / Q000302:isolation & purification; D011424:Propionibacterium / Q000302:isolation & purification; D011671:Pulpitis / Q000382:microbiology*; D013210:Staphylococcus / Q000302:isolation & purification; D013291:Streptococcus / Q000302:isolation & purification; D014678:Veillonella / Q000302:isolation & purification
chemical_list: D015342:DNA Probes; D004269:DNA, Bacterial