Incidence of human papilloma virus in patients with invasive cervical carcinoma.
The dot-blot hybridization of biotin-dUTP-labeled HPV-16 DNA with genomic DNA extracted from biopsies taken from patients with invasive carcinoma and abnormal cytology showed the presence of HPV-DNA in 88% and 80% cases under relaxed conditions and 40% and 20% cases under stringent conditions of hybridization, respectively. Southern blot revealed the HPV-DNA in randomly integrated form in two cases and in episomal form in the other two.
Sobti
R C
RC
Department of Biotechnology, Panjab University, Chandigarh, India.
Capalash
N
N
Sehgal
S
S
Gupta
B D
BD
Sharma
P
P
D015139:Blotting, Southern; D004279:DNA, Viral / Q000302:isolation & purification*; D005260:Female; D006801:Humans; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D002583:Uterine Cervical Neoplasms / Q000821:virology*
D004279:DNA, Viral