Analysis of lipopolysaccharide-response genes in B-lineage cells demonstrates that they can have differentiation stage-restricted expression and contain SH2 domains.
Bacterial lipopolysaccharide (LPS) is a potent stimulator of B-cell activation, proliferation, and differentiation. We examined the genetic response of B-lineage cells to LPS via trapping of expressed genes with a gene-trap retrovirus. This analysis showed that expression of only a small fraction of genes is altered during LPS stimulation of B-lineage cells. Isolation of the cellular portion of the trapped LPS-response genes via 5' RACE (rapid amplification of cDNA ends) cloning identified novel genes for all the cloned loci. These novel LPS-response genes were also found to have differentiation stage-restricted expression within the B-lymphoid lineage. That LPS-response genes in B cells also have differentiation stage-restricted expression suggests that these genes may be involved in the control of B-cell function and differentiation, since the known members of this class of genes have frequently been found to play a role in the function and differentiation of B-lineage cells. The isolation of novel members of this class of genes, including a gene that contains a putative SH2 domain, will further increase our understanding of the molecular events involved in the control of B-cell differentiation and function.
Kerr
W G
WG
Department of Genetics, Stanford University School of Medicine, CA 94305, USA.
Heller
M
M
Herzenberg
L A
LA
D000595:Amino Acid Sequence; D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology / Q000502:physiology*; D001483:Base Sequence; D002454:Cell Differentiation; D002460:Cell Line; D018920:Coculture Techniques; D017931:DNA Primers; D005091:Exons; D005786:Gene Expression Regulation; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008213:Lymphocyte Activation; D008969:Molecular Sequence Data; D015688:Oncogene Protein pp60(v-src) / Q000737:chemistry; D016366:Open Reading Frames; D016133:Polymerase Chain Reaction; D016315:Proto-Oncogene Proteins c-abl / Q000737:chemistry; D011994:Recombinant Proteins / Q000096:biosynthesis; D017386:Sequence Homology, Amino Acid; D014162:Transfection; D001616:beta-Galactosidase / Q000096:biosynthesis; D018909:src Homology Domains
D017931:DNA Primers; D008070:Lipopolysaccharides; D011994:Recombinant Proteins; D015688:Oncogene Protein pp60(v-src); D016315:Proto-Oncogene Proteins c-abl; D001616:beta-Galactosidase