title: Involvement of oxidative stress in D-xylose-induced cataractogenesis in cultured rat lenses.
abstract: In order to clarify the involvement of oxidative stress in in vitro sugar-induced cataractogenesis, we examined changes in lipid peroxide, reduced glutathione, vitamin E, and water contents following cataractogenesis in rat lenses cultured with 20 mM D-xylose over a period of 24 h. In D-xylose-treated lenses, an apparent opacity appeared at the equator after 6 h of D-xylose treatment, increases in lipid peroxide and water contents and decreases in reduced glutathione and vitamin E contents occurred with the appearance of opacity. We further examined the effect of the treatment of vitamin E in a liposomal form on cataractogenesis and the changes of lipid peroxide, reduced glutathione, vitamin E, water, and xylitol contents in rat lenses cultured with 20 mM D-xylose for 24 h. This vitamin E treatment significantly prevented not only cataractogenesis, but also an increase in lipid peroxide content and a decrease in vitamin E content in the D-xylose-treated lenses. However, the vitamin E treatment had no effect not only on a decrease in reduced glutathione content, but also on increases in water and xylitol contents, which are known to be induced via aldose reductase, in the D-xylose-treated lenses. These results indicate that not only osmotic stress but also oxidative stress should be involved in cataractogenesis in rat lenses cultured with D-xylose and support the involvement of oxidative stress in in vitro sugar-induced cataractogenesis.
authors.affiliation: Department of Biochemistry, School of Medicine, Fujita Health University, Aichi, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Ohta
authors.forename: H
authors.initials: H
authors.lastname: Torii
authors.forename: H
authors.initials: H
authors.lastname: Okada
authors.forename: H
authors.initials: H
authors.lastname: Hattori
authors.forename: Y
authors.initials: Y
authors.lastname: Majima
authors.forename: I
authors.initials: I
authors.lastname: Ishiguro
mesh_terms: D000818:Animals; D001834:Body Water / Q000378:metabolism; D002386:Cataract / Q000139:chemically induced / Q000378:metabolism* / Q000517:prevention & control; D003470:Culture Media; D046508:Culture Techniques; D004337:Drug Carriers; D005978:Glutathione / Q000378:metabolism; D007908:Lens, Crystalline / Q000187:drug effects; D008054:Lipid Peroxides / Q000378:metabolism; D008081:Liposomes; D008297:Male; D018384:Oxidative Stress / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D014810:Vitamin E / Q000378:metabolism / Q000494:pharmacology; D014993:Xylitol / Q000378:metabolism; D014994:Xylose / Q000494:pharmacology
chemical_list: D003470:Culture Media; D004337:Drug Carriers; D008054:Lipid Peroxides; D008081:Liposomes; D014810:Vitamin E; D014994:Xylose; D005978:Glutathione; D014993:Xylitol