Envelope gene sequences of human T-lymphotropic virus type 1 in Taiwan.
Three major types of HTLV-1 had been proposed, the Melanesian type, the Zairian type, and the cosmopolitan type, which was further divided into subtypes A, B and C, according to the phylogenetic tree constructed from LTR sequences of current HTLV-isolates. In this study, the envelope gene sequences of HTLV-1 from 9 Taiwanese were analyzed. Based on the phylogenetic tree constructed by unweighted pair group method and the sequence homology analysis by GCG computer programs, the envelope gene sequences of HTLV-1 proviruses from these 9 Taiwanese belonged to subtype A or subtype B of the cosmopolitan type and were closely related to HTLV-1 from Japan. Twelve subtype-specific nucleotide variations were deduced from the comparison of complete or partial envelope gene sequences of 16 HTLV-1 isolates of known subtypes as well as those of 9 Taiwanese. These data provided the basis for subtyping the cosmopolitan type of HTLV-1 by amplification of envelope gene sequences and restriction fragment length polymorphism studies. A more extensive survey based upon this proposal was warranted.
Lin
M T
MT
Department of Oncology, National Taiwan University Hospital, Taipei.
Chen
Y C
YC
Chen
P J
PJ
Yang
Y C
YC
Tang
J L
JL
Wu
J M
JM
Chuang
S E
SE
Yang
C S
CS
D001483:Base Sequence; D017931:DNA Primers; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D015752:Genes, env; D014644:Genetic Variation; D015490:HTLV-I Infections / Q000453:epidemiology; D015368:Human T-lymphotropic virus 1 / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D007564:Japan; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D015995:Prevalence; D012689:Sequence Homology, Nucleic Acid; D012984:Software; D013624:Taiwan / Q000453:epidemiology
D017931:DNA Primers; D004279:DNA, Viral