Localization of highly repetitive, species-specific EcoRI elements from Lupinus luteus L.
Using Southern, dot-blot and in situ hybridization, molecular and cytological localization of repetitive Lupinus luteus DNA sequence was shown. Under CsCl gradient centrifugation conditions CG-rich satellite fraction appeared. Dot-blot hybridization clearly indicated that 1070bp repetitive element being a member of previously described EcoRI fragments family appeared only in the main band. The use of that DNA fragment as an in situ hybridization probe revealed the hybridization signal in the euchromatin area.
Sakowicz
T
T
Department of Plant Cytology and Cytochemistry, University of Lódź, Poland.
Luchniak
P
P
D015139:Blotting, Southern; D002467:Cell Nucleus / Q000235:genetics; D002586:Cesium; D002712:Chlorides; D018744:DNA, Plant / Q000032:analysis; D004276:DNA, Satellite / Q000032:analysis; D015246:Deoxyribonuclease EcoRI; D015151:Immunoblotting; D017403:In Situ Hybridization; D007399:Interphase / Q000235:genetics; D018517:Plant Roots / Q000235:genetics; D010944:Plants / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*; D012639:Seeds / Q000235:genetics; D016622:Silver Staining
D002712:Chlorides; D018744:DNA, Plant; D004276:DNA, Satellite; D002586:Cesium; D015246:Deoxyribonuclease EcoRI; C028019:cesium chloride