title: Epidermal differentiation in the human external auditory meatus.
abstract: The differentiation of epidermis in the various parts of the human ear canal was documented on the basis of cytokeratin (Ck) expression patterns. Immunohistochemistry was performed on cryostat sections of normal meatal skin using a comprehensive panel of monospecific Ck antibodies representing the main lines of epithelial differentiation. The epidermis of the cartilaginous part showed a Ck profile characteristic of normal skin type differentiation. The deep meatal skin, including the tympanic membrane, showed a peculiar type of differentiation: in addition to epidermal Cks, hyperproliferation-associated Cks 6, 16, and 17 were expressed in the suprabasal cells, while the simple epithelia cell marker Ck 19 was found in the basal cells. The presence of hyperproliferative Cks in the deep meatal skin could only partly be related to areas of proliferative activity. Keratinocytes, which express markers of hyperproliferation, are migratory. Therefore, their presence in the meatal skin is likely to be related to the peculiar pattern of keratinocyte migration, the purpose of which is to keep the meatus free from desquamation products.
authors.affiliation: Department of Otorhinolaryngology, University of Nijmegen, The Netherlands.
authors.forename: P P
authors.initials: PP
authors.lastname: Vennix
authors.forename: W
authors.initials: W
authors.lastname: Kuijpers
authors.forename: T A
authors.initials: TA
authors.lastname: Peters
authors.forename: E L
authors.initials: EL
authors.lastname: Tonnaer
authors.forename: F C
authors.initials: FC
authors.lastname: Ramaekers
mesh_terms: D002356:Cartilage / Q000166:cytology / Q000378:metabolism; D002454:Cell Differentiation; D004424:Ear Canal / Q000166:cytology* / Q000378:metabolism; D000078404:Epidermal Cells; D004817:Epidermis / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D015603:Keratinocytes / Q000166:cytology / Q000378:metabolism; D007633:Keratins / Q000378:metabolism; D012016:Reference Values; D014432:Tympanic Membrane / Q000166:cytology / Q000378:metabolism
chemical_list: D007633:Keratins