Determination of chloramphenicol in muscle, liver, kidney and urine of pigs by means of immunoaffinity chromatography and gas chromatography with electron-capture detection.
A rapid and specific clean-up procedure based on immunoaffinity chromatography (IAC) with polyclonal antibodies for the gas chromatographic determination with electron-capture detection of chloramphenicol in pig muscle tissue, organs and urine is described. A commercially available IAC material was used for the analysis. A decrease in the capacity of the column after being used more than 100 times was observed. Mean recoveries were 69, 54, 62 and 95% for spiked pig muscle tissue, liver, kidney and urine, respectively. The limit of detection was 0.2 micrograms/kg for muscle tissue, 2.0 micrograms/kg for liver and kidney and 0.4 micrograms/kg for urine.
Gude
T
T
Bundesinstitut für gesundheitlichen Verbraucherschutz, Berlin, Germany.
Preiss
A
A
Rubach
K
K
D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis / Q000652:urine; D000906:Antibodies; D002701:Chloramphenicol / Q000032:analysis* / Q000652:urine; D002846:Chromatography, Affinity / Q000379:methods* / Q000706:statistics & numerical data; D002849:Chromatography, Gas / Q000379:methods* / Q000706:statistics & numerical data; D007202:Indicators and Reagents; D007668:Kidney / Q000737:chemistry*; D008099:Liver / Q000737:chemistry*; D008433:Mathematics; D008832:Microchemistry; D009132:Muscles / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013552:Swine
D000900:Anti-Bacterial Agents; D000906:Antibodies; D007202:Indicators and Reagents; D002701:Chloramphenicol