Polymorphonuclear leukocytes present laminin peptides in endocytic compartments.
Rat and Human neutrophils presented cytoplasmic vacuoles immunoreactive for laminin at the electron microscopy level. Colocalization of the anti-laminin labeling with albumin-gold complexes and alkaline phosphatase activity in rat PMN suggest an endocytic nature for this compartment. Immunoblot analysis of human normal peripheral blood neutrophils revealed the presence of two laminin peptides around 100 kDa.
Vannier-Santos
M A
MA
Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Brasil. vannier@ibccf.biof.ufrj.br
de Souza
S J
SJ
Brentani
R R
RR
de Souza
W
W
D000818:Animals; D002451:Cell Compartmentation; D004705:Endocytosis; D006801:Humans; D007150:Immunohistochemistry; D007797:Laminin / Q000378:metabolism*; D009504:Neutrophils / Q000378:metabolism*; D051381:Rats
D007797:Laminin