Characterization of a cDNA and gene encoding the mouse theta class glutathione transferase mGSTT2 and its localization to chromosome 10B5-C1.
In this study, we have isolated and characterized a gene and cDNA encoding a mouse Theta class GST. The gene, mGSTT2, spans approximately 3.1 kb and is composed of five exons interrupted by four introns. The gene was localized to Chromosome 10B5-C1 by in situ hybridization. Southern blot analysis of mouse genomic DNA suggests that there is only one copy of mGSTT2 in the mouse genome. The cDNA derived from mGSTT2 was isolated from a mouse liver cDNA library and has an open reading frame of 732 bp encoding a peptide of 244 amino acids with a calculated molecular weight of 26,676 Da. The encoded protein shares amino acid sequence identities of 92, 77, 51, and 55% with rat subunit Yrs, human subunit GSTT2, rat subunit 5, and human subunit GSTT1, respectively.
Whittington
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AT
Molecular Genetics Group,John Curtin School of Medical Research, Australian National University, Canberra, Australian Capital Territory, 2601, Australia.
Webb
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GC
Baker
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RT
Board
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PG
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D018076:DNA, Complementary / Q000235:genetics; D005796:Genes; D005982:Glutathione Transferase / Q000235:genetics*; D006801:Humans; D017403:In Situ Hybridization; D007438:Introns; D051379:Mice; D008969:Molecular Sequence Data; D051381:Rats; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; C484260:Gstt2 protein, mouse; C091152:Gstt2 protein, rat; D005982:Glutathione Transferase