Characterisation of the mcpA and mcpB genes capable of encoding methyl-accepting type chemoreceptors in Rhodobacter capsulatus.
Two contiguous mcp genes, mcpA and mcpB, transcribed from the same DNA strand and capable of encoding methyl-accepting chemotaxis proteins (Mcp) have been isolated from Rhodobacter capsulatus (Rc), sequences and overexpressed in Escherichia coli (Ec). The deduced proteins (McpA, 69 171 Da; McpB, 81 629 Da) show a structure similar to that of Ec Mcp. The products of mcpA and mcpB, overproduced in Ec, were recognized by anti-Ec Mcp (Trg) antibodies.
Michotey
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CEA/Grenoble, Laboratoire de Biochimie Microbienne, CNRS URA 1130 alliée à l'INSERM, DBMS, Grenoble, France.
Toussaint
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Richaud
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Vignais
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D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology; D001483:Base Sequence; D002628:Chemoreceptor Cells; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D005798:Genes, Bacterial; D008565:Membrane Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology; D000072236:Methyl-Accepting Chemotaxis Proteins; D008745:Methylation; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016354:Rhodobacter capsulatus / Q000235:genetics* / Q000276:immunology; D017385:Sequence Homology; D013045:Species Specificity
D001426:Bacterial Proteins; D008565:Membrane Proteins; D000072236:Methyl-Accepting Chemotaxis Proteins