title: Localization of the expression of complement component 3 in the human endometrium by in situ hybridization.
abstract: C3 production by the human endometrium has been previously described. The objective of the current study was to localize the site of expression and regulation of the third component of complement, C3, in the endometrium. Eight secretory and eight proliferative archival endometrial samples from hysterectomy and endometrial biopsy specimens were used for in situ hybridization analysis. This analysis was performed with a radiolabeled riboprobe synthesized from a 736-bp template representing sequence 1944-2680 of the human C3 complementary DNA. Duplicate sections were hybridized with sense and antisense riboprobes. Resultant autoradiograms were analyzed qualitatively by light- and darkfield microscopy. In proliferative endometrium, minimal expression of C3 was observed and was limited to a few stromal patches and glands throughout the section. In the secretory samples, prominent C3 expression was observed in both the glands and stroma of the basalis layer. Endometrial lymphocytes did not express C3. Endometrial stromal and glandular cells express the C3 gene. Endometrial lymphocytes did not express C3, but other nondistinct lymphoid elements scattered in the stroma may be expressing C3. There was a visibly more intense expression of C3 in the basalis layer of the secretory endometrium than in proliferative endometrium. The spatial and temporal pattern of C3 expression may have implications in normal menstrual physiology and in the immunological response of the endometrium to the invading trophoblast during placentation.
authors.affiliation: Vincent Memorial Obstetrics and Gynecology Service, Massachusetts General Hospital, Boston, USA.
authors.forename: R A
authors.initials: RA
authors.lastname: Sayegh
authors.forename: X J
authors.initials: XJ
authors.lastname: Tao
authors.forename: J T
authors.initials: JT
authors.lastname: Awwad
authors.forename: K B
authors.initials: KB
authors.lastname: Isaacson
mesh_terms: D000328:Adult; D001706:Biopsy; D003176:Complement C3 / Q000096:biosynthesis*; D004715:Endometriosis / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D004717:Endometrium / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D005260:Female; D015870:Gene Expression; D005786:Gene Expression Regulation; D006801:Humans; D007044:Hysterectomy; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008875:Middle Aged; D015347:RNA Probes
chemical_list: D003176:Complement C3; D015347:RNA Probes