title: Schistosoma mansoni infection in humans and primates induces cytolytic antibodies to surface Le(x) determinants on myeloid cells.
abstract: The Lewis x antigen (Le(x); Gal beta 1-4[Fuc alpha 1-3]GlcNac beta1-R), which is present on the surfaces of human cells, is also synthesized by the human helminthic parasite Schistosoma mansoni. We now report that IgM and IgG antibodies to Le(x) antigens are present in the sera of humans and rhesus monkeys infected with S. mansoni, whereas these antibodies are completely absent in uninfected individuals. The sera from infected humans and monkeys mediate specific complement-dependent cytolysis of human promyelocytic leukemic HL-60 cells, which bear surface Le(x) antigen. Furthermore, the major activity in sera from infected individuals toward HL-60 cells is due to anti-Le(x) reactivity.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City 73104, USA.
authors.forename: A K
authors.initials: AK
authors.lastname: Nyame
authors.forename: J B
authors.initials: JB
authors.lastname: Pilcher
authors.forename: V C
authors.initials: VC
authors.lastname: Tsang
authors.forename: R D
authors.initials: RD
authors.lastname: Cummings
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis* / Q000097:blood / Q000276:immunology; D003165:Complement System Proteins / Q000276:immunology; D003602:Cytotoxicity, Immunologic; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D007075:Immunoglobulin M / Q000097:blood / Q000276:immunology; D015473:Leukemia, Promyelocytic, Acute; D016256:Lewis X Antigen / Q000276:immunology*; D008253:Macaca mulatta; D012550:Schistosoma mansoni / Q000276:immunology*; D012555:Schistosomiasis mansoni / Q000276:immunology*; D014407:Tumor Cells, Cultured
chemical_list: D000909:Antibodies, Helminth; D007106:Immune Sera; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D016256:Lewis X Antigen; D003165:Complement System Proteins