title: Studies of human and rat blood under oxidative stress: changes in plasma thiol level, antioxidant enzyme activity, protein carbonyl content, and fluidity of erythrocyte membrane.
abstract: Previously by selection and inbreeding of Wistar rats susceptible or resistant to the cataractogenic effect of galactose the S and R rat strains were developed as a model of oxidative stress. It was earlier found S rats have elevated level of OH-radical generation and enhanced lipid peroxidation compared with that for R rats. Our data show that superoxide dismutase and catalase activities in the blood of S rats are twice lower than corresponding values of R rats. Increased level of disulfides and protein carbonyl groups was obtained in the blood plasma of S-rats. It was found that fluidity of erythrocyte membrane measured by photobleaching method is definitely higher for S rats compared with that for S rats. Similar effect of decreased catalase activity and increased disulfide content has been observed for kyphoscoliosis patients.
authors.affiliation: Institute of Chemical Kinetics and Combustion, Novosibirsk, Russia.
authors.forename: V
authors.initials: V
authors.lastname: Yelinova
authors.forename: Y
authors.initials: Y
authors.lastname: Glazachev
authors.forename: V
authors.initials: V
authors.lastname: Khramtsov
authors.forename: L
authors.initials: L
authors.lastname: Kudryashova
authors.forename: V
authors.initials: V
authors.lastname: Rykova
authors.forename: R
authors.initials: R
authors.lastname: Salganik
mesh_terms: D000818:Animals; D001798:Blood Proteins / Q000737:chemistry / Q000378:metabolism*; D002374:Catalase / Q000097:blood*; D004910:Erythrocyte Membrane / Q000378:metabolism; D006801:Humans; D008297:Male; D008560:Membrane Fluidity; D018384:Oxidative Stress; D051381:Rats; D017208:Rats, Wistar; D013438:Sulfhydryl Compounds / Q000097:blood*; D013482:Superoxide Dismutase / Q000097:blood*
chemical_list: D001798:Blood Proteins; D013438:Sulfhydryl Compounds; D002374:Catalase; D013482:Superoxide Dismutase