Plasmalogens and their oxidative degradation products in low and high density lipoprotein.
The kinetics of the autoxidation reaction of LDL and HDL with Fe2+/ascorbate was investigated. Aldehydes derived from plasmalogens were determined by conversion to 2-alkyl-1,3-dithiolanes. Their oxidation products, 2-hydroxy-aldehydes, were trapped by reaction with pentafluorobenzylhydroxylamine. After derivatisation, the pentafluorobenzyloximes could be separated from other compounds by thin-layer chromatograpy. They were detected by gas chromatography applying an electron capture detector. LDL plasmalogens are oxidized nearly completely after 180 min while HDL plasmalogens suffer oxidation only to 5%. Oxidation of linoleic and arachidonic acid was investigated by simultaneous determination of 2-hydroxyheptanal.
Jira
W
W
Lehrstuhl Organische Chemie I, Universität Bayreuth, Germany.
Spiteller
G
G
D000818:Animals; D005069:Evaluation Studies as Topic; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D066298:In Vitro Techniques; D007700:Kinetics; D015227:Lipid Peroxidation; D008075:Lipoproteins, HDL / Q000737:chemistry / Q000378:metabolism*; D008077:Lipoproteins, LDL / Q000737:chemistry / Q000378:metabolism*; D010084:Oxidation-Reduction; D010955:Plasmalogens / Q000737:chemistry / Q000378:metabolism*; D013552:Swine
D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D010955:Plasmalogens