title: The pharmacokinetics, bioavailability and biodistribution in mice of a rationally designed 2-nitroimidazole hypoxia probe SR-4554.
abstract: N-(2-Hydroxy-3,3,3-trifluoropropyl)-2-(2-nitro-1-imidazolyl) acetamide (SR-4554) is a fluorinated 2-nitroimidazole which has been rationally designed as a non-invasive probe for tumor hypoxia. The key selection criteria for this molecule were low central nervous system penetration and toxicity, high metabolic stability other than nitroreduction, good tumor uptake and high sensitivity for detection by magnetic resonance spectroscopy. As part of the pre-clinical development strategy, pharmacokinetic, bioavailability and biodistribution studies were performed in mice. Pharmacokinetic studies in mice demonstrated that SR-4554 was rapidly absorbed into plasma following i.p. administration and eliminated with a half-life of 42 min, similar to other 2-nitroimidazoles. By comparing the areas under the concentration-time curve (AUC), the tumor exposure towards SR-4554 was on average 84% of the value obtained for the plasma exposure. SR-4554 penetrated tumor tissue extremely well but, in contrast to misonidazole and certain other fluorinated analogues, its distribution into brain tissue was poor (AUCbrain/AUCplasma = 0.07), suggesting potentially lower toxicity in spite of its higher lipophilicity (P = 0.43 versus 0.63, respectively). The bioavailability of SR-4554 from i.p. and p.o. routes was 100 and 96% respectively. In non-tumor-bearing mice, SR-4554 was excreted mainly as unchanged drug. The percentage of the injected i.p. dose of SR-4554 excreted unchanged in the urine over 24 h was 68 +/- 8%. Neither SR-4554 nor its metabolites were detected in mouse feces. We propose that these favorable pharmacokinetic properties of SR-4554 are due to the hydrophilic character and hydrogen-bonding capability of the amide and hydroxyl functions in the compound.
authors.affiliation: CRC Department of Medical Oncology, University of Glasgow, Beatson Laboratories, UK.
authors.forename: E O
authors.initials: EO
authors.lastname: Aboagye
authors.forename: A D
authors.initials: AD
authors.lastname: Lewis
authors.forename: M A
authors.initials: MA
authors.lastname: Graham
authors.forename: M
authors.initials: M
authors.lastname: Tracy
authors.forename: A B
authors.initials: AB
authors.lastname: Kelson
authors.forename: K J
authors.initials: KJ
authors.lastname: Ryan
authors.forename: P
authors.initials: P
authors.lastname: Workman
mesh_terms: D000818:Animals; D001682:Biological Availability; D001921:Brain / Q000378:metabolism; D015687:Cell Hypoxia; D005260:Female; D008099:Liver / Q000378:metabolism; D008325:Mammary Neoplasms, Experimental / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D015335:Molecular Probes; D009368:Neoplasm Transplantation; D009593:Nitroimidazoles / Q000493:pharmacokinetics* / Q000652:urine; D014018:Tissue Distribution
chemical_list: D015335:Molecular Probes; D009593:Nitroimidazoles; C095171:SR 4554