Transcription of the rat angiotensin II type 2 receptor gene.
The promoter region of the rat angiotensin II type 2 receptor gene was cloned and the nucleotide sequences were determined. A computer homology search for a 1.2 Kb promoter region showed that there are several consensus cis DNA elements such as C/EBP, NF-IL6, GRE and AP1 in this region. Primer extension experiments showed that there is one transcription initiation site 15 bp-downstream of the TATA box. Deletion mutants of the 1.2 Kb segment were prepared and fused to a luciferase reporter gene. These type 2 receptor promoter-luciferase constructs were introduced into PC12W cells, a pheochromocytoma cell line expressing the type 2 receptor, and luciferase activity was measured. It showed that (1) a DNA segment between -1208 bp and -749 bp suppresses the promoter activity of type 2 receptor gene, (2) a positive regulatory element is present in a DNA segment between -749 bp and -216 bp; and (3) a DNA segment between -44 bp +58 bp is important for the basal promoter activity of the type 2 receptor gene.
Ichiki
T
T
Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA.
Kambayashi
Y
Y
Inagami
T
T
D000310:Adrenal Gland Neoplasms; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D003001:Cloning, Molecular; D016384:Consensus Sequence; D004268:DNA-Binding Proteins / Q000378:metabolism; D008156:Luciferases / Q000096:biosynthesis; D008969:Molecular Sequence Data; D016716:PC12 Cells; D010673:Pheochromocytoma; D011401:Promoter Regions, Genetic; D051381:Rats; D044139:Receptor, Angiotensin, Type 2; D011945:Receptors, Angiotensin / Q000096:biosynthesis* / Q000235:genetics*; D011994:Recombinant Proteins / Q000096:biosynthesis; D012045:Regulatory Sequences, Nucleic Acid; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic; D014162:Transfection
D004268:DNA-Binding Proteins; D044139:Receptor, Angiotensin, Type 2; D011945:Receptors, Angiotensin; D011994:Recombinant Proteins; D008156:Luciferases