title: The defective secretion of a naturally occurring alpha-1-antichymotrypsin variant with a frameshift mutation.
abstract: A newly found variant alpha-1-antichymotrypsin (ACT), ACT Isehara-2, has a deletion of two bases (AA) at codon 391 near the carboxyl terminus. This frameshift mutation caused a change in the amino acid sequence and generated 10 extra amino acids (408 amino acids total) [Tsuda, M., Sei, Y., Matsumoto, M., Kamiguchi, H., Yamamoto, Y., Shinohara, Y., Igarashi, T. & Yamamura, M. (1992) Hum. Genet. 91. 467-468]. The serum ACT levels in three unrelated heterozygotes with this mutant ACT gene were 37% 49% and 54% that of the normal individuals. To examine the reduced serum levels, the normal ACT and the mutant ACT created by site-directed mutagenesis were transfected into COS-7 cells for comparison. The value for the retention rate (intracellular ACT/total ACT) was apparently higher in the cells expressing mutant ACT Isehara-2 than those bearing the normal gene. In the pulse-chase experiments, the secretion of the synthesized mutant ACT into the medium was not observed, whereas the normal ACT was mostly secreted as a 64-kDa form. The endoglycosidase H digestion and an electron microscopic analysis indicated that the retained mutant ACT was present in the endoplasmic reticulum. These results provide the biochemical basis for the decreased serum ACT level of individuals with ACT Isehara-2, and suggest the importance of the carboxyl-terminal region for its secretion.
authors.affiliation: Department of Molecular Life Science, Tokai University, School of Medicine, Kanagawa, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Tsuda
authors.forename: Y
authors.initials: Y
authors.lastname: Sei
authors.forename: T
authors.initials: T
authors.lastname: Ohkubo
authors.forename: M
authors.initials: M
authors.lastname: Yamamura
authors.forename: H
authors.initials: H
authors.lastname: Kamiguchi
authors.forename: A
authors.initials: A
authors.lastname: Akatsuka
authors.forename: T
authors.initials: T
authors.lastname: Tsuda
authors.forename: H
authors.initials: H
authors.lastname: Tachikawa
authors.forename: M
authors.initials: M
authors.lastname: Yamamoto
authors.forename: Y
authors.initials: Y
authors.lastname: Shinohara
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003470:Culture Media; D018076:DNA, Complementary; D016521:Electrophoresis, Gel, Pulsed-Field; D016368:Frameshift Mutation; D006801:Humans; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D014162:Transfection; D000514:alpha 1-Antichymotrypsin / Q000097:blood / Q000235:genetics* / Q000378:metabolism*
chemical_list: D003470:Culture Media; D018076:DNA, Complementary; D000514:alpha 1-Antichymotrypsin