Structures of diphospho-myo-inositol pentakisphosphate and bisdiphospho-myo-inositol tetrakisphosphate from Dictyostelium resolved by NMR analysis.
Diphospho-myo-inositol phosphates (PP-InsP5 and bis-PP-InsP4) were isolated from Dictyostelium in order to clarify the precise positional isomerism by two-dimensional 1H/31P-NMR analysis. The diphosphorylated inositol phosphates are 4-PP-Ins(1,2,3,5,6)P5 and 4,5-bis-PP-Ins(1,2,3,6)P4 or their corresponding enantiomers. The vicinal arrangement of the diphospho groups with its steric and electrostatic constraints possibly qualifies bis-PP-InsP4 as a metabolite with high phosphate-group-transfer potential in phosphotransferase reactions.
Laussmann
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Fachbereich 9-Chemie, Bergische Universität GHS Wuppertal, Federal Republic of Germany.
Eujen
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R
Weisshuhn
C M
CM
Thiel
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U
Vogel
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G
D000818:Animals; D004023:Dictyostelium / Q000737:chemistry*; D006859:Hydrogen / Q000737:chemistry; D007295:Inositol Phosphates / Q000737:chemistry* / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D010759:Phosphorus Isotopes
D007295:Inositol Phosphates; D010759:Phosphorus Isotopes; C079849:1-diphosphoinositol pentakisphosphate; C079848:bis(1,4)-diphosphoinositol tetrakisphosphate; D006859:Hydrogen