Vasodilation induced by vasopressin V2 receptor stimulation in afferent arterioles.
We have previously reported that vasopressin (AVP) V2 receptor stimulation increased renal blood flow in dogs anesthetized with pentobarbital. In this study, we examined the direct effects of AVP on afferent arterioles to clarify the role played by V2 receptors in regulating afferent arteriolar tone. We microdissected a superficial afferent arteriole with glomerulus from the kidney of a New Zealand White rabbit. Each afferent arteriole was cannulated with a pipette system and microperfused in vitro at 60 mm Hg. The effects of vasoactive substances were evaluated by changes in the lumen diameter of afferent arterioles. We found that AVP decreased the lumen diameter of microperfused afferent arterioles dose-dependently and that a V1 antagonist, OPC21268, inhibited the vasoconstrictor action of AVP. However, AVP 10(-8) M increased the lumen diameter of norepinephrine (NE)-constricted afferent arterioles pretreated with OPC21268 (OPC + NE, 8.2 +/- 0.7 microns; OPC + NE + AVP, 9.9 +/- 0.9 microns*; *P < 0.05, N = 13). This vasodilatory effect of AVP was abolished by pretreatment with a V2 antagonist, OPC31260. Desmopressin (dDAVP), a V2 agonist, increased the lumen diameter of the NE-constricted afferent arterioles (NE, 7.4 +/- 0.9 microns; NE + dDAVP, 10.1 +/- 0.7 microns*; *P < 0.05, N = 9). These results suggest that AVP V2 receptors are present in rabbit afferent arterioles and that V2 receptor stimulation induces vasodilation in rabbit afferent arterioles.
Tamaki
T
T
Department of Pharmacology, Kagawa Medical School, Japan.
Kiyomoto
K
K
He
H
H
Tomohiro
A
A
Nishiyama
A
A
Aki
Y
Y
Kimura
S
S
Abe
Y
Y
D000818:Animals; D065092:Antidiuretic Hormone Receptor Antagonists; D001160:Arterioles / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D003894:Deamino Arginine Vasopressin / Q000494:pharmacology; D007678:Kidney Glomerulus / Q000098:blood supply*; D008297:Male; D009638:Norepinephrine / Q000494:pharmacology; D010477:Perfusion; D010880:Piperidines / Q000494:pharmacology; D015363:Quinolones / Q000494:pharmacology; D011817:Rabbits; D017483:Receptors, Vasopressin / Q000502:physiology*; D012076:Renal Agents / Q000494:pharmacology; D014662:Vasoconstrictor Agents / Q000494:pharmacology; D014664:Vasodilation / Q000187:drug effects / Q000502:physiology*
D065092:Antidiuretic Hormone Receptor Antagonists; D010880:Piperidines; D015363:Quinolones; D017483:Receptors, Vasopressin; D012076:Renal Agents; D014662:Vasoconstrictor Agents; C068644:OPC 21268; D003894:Deamino Arginine Vasopressin; D009638:Norepinephrine