title: Pharmacokinetics and biodistribution of oligonucleotide adsorbed onto poly(isobutylcyanoacrylate) nanoparticles after intravenous administration in mice.
abstract: OBJECTIVE
  The goal of this study was to evaluate the ability of nanoparticles to be used as a targeted delivery system for oligonucleotides.
  
  
  METHODS
  Pharmacokinetic and tissue distribution were carried out in mice by measuring radioactivity associated to the model oligothymidylate 33P-pdT16 loaded to poly(isobutylcyanoacryate) (PIBCA) nanoparticles. In addition, we have used a TLC linear analyzer to measure quantitatively on a polyacrylamide gel electrophoresis, the amount of non degraded pdT16.
  
  
  RESULTS
  Organ distribution study has shown that nanoparticles deliver 33P-pdT16 specifically to the liver reducing its distribution in the kidney and in the bone marrow. Nanoparticles could partially protect pdT16 against degradation in the plasma and in the liver 5 min after administration, whereas free oligonucleotide was totally degraded at the same time.
  
  
  CONCLUSIONS
  Nanoparticles protect oligonucleotides in vivo against degradation and deliver them to the liver.
authors.affiliation: Pharmaceuticals Research Center, Kanebo, LTD., Osaka, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Nakada
authors.forename: E
authors.initials: E
authors.lastname: Fattal
authors.forename: M
authors.initials: M
authors.lastname: Foulquier
authors.forename: P
authors.initials: P
authors.lastname: Couvreur
mesh_terms: D000327:Adsorption; D000818:Animals; D003487:Cyanoacrylates / Q000737:chemistry*; D004337:Drug Carriers; D004355:Drug Stability; D004659:Enbucrilate; D005069:Evaluation Studies as Topic; D007275:Injections, Intravenous; D008099:Liver / Q000378:metabolism; D051379:Mice; D008815:Mice, Inbred Strains; D008863:Microspheres; D010316:Particle Size; D011108:Polymers / Q000737:chemistry*; D013942:Thymine Nucleotides / Q000737:chemistry / Q000493:pharmacokinetics*; D014018:Tissue Distribution / Q000502:physiology
chemical_list: D003487:Cyanoacrylates; D004337:Drug Carriers; D011108:Polymers; D013942:Thymine Nucleotides; D004659:Enbucrilate