Identification and characterization of novel dermal Thy-1 antigen-bearing dendritic cells in murine skin.
Using immunofluorescence on dermal sheets of mouse ear, we identified novel Thy-1+ dermal dendritic cells in murine skin. These cells are resistant to topical corticosteroid treatment. The number of these dermal Thy-1+ dendritic cells is not altered with aging of the mice or with 3 d of culture. Dermal Thy-1+ dendritic cells have melanosomes in their cytoplasm; hence, phagocytic activity is likely to be present. Double immunofluorescence revealed that most of these dermal Thy-1+ dendritic cells express CD45. Furthermore, conventional immunofluorescence and confocal laser scanning microscopic findings showed that most of these Thy-1+ dermal dendritic cells express gamma delta and V gamma 3 T-cell receptors. The relationship between these dermal Thy-1+ dendritic cells and dendritic epidermal T cells is discussed.
Tamaki
K
K
Department of Dermatology, Yamanashi Medical University and The University of Tokyo, Japan.
Yasaka
N
N
Chang
C H
CH
Ohtake
N
N
Saitoh
A
A
Nakamura
K
K
Furue
M
M
D000818:Animals; D003713:Dendritic Cells / Q000276:immunology* / Q000648:ultrastructure; D005260:Female; D005455:Fluorescent Antibody Technique; D007150:Immunohistochemistry; D051379:Mice; D008806:Mice, Inbred AKR; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D018613:Microscopy, Confocal; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000378:metabolism; D012867:Skin / Q000166:cytology / Q000276:immunology*; D018800:Thy-1 Antigens / Q000378:metabolism*
D016692:Receptors, Antigen, T-Cell, gamma-delta; D018800:Thy-1 Antigens