Interleukin-4 upregulates the heat shock protein Hsp90alpha and enhances transcription of a reporter gene coupled to a single heat shock element.
Transcription of the heat shock protein Hsp90alpha was strongly upregulated in human T-cells by interleukin-4 (IL-4) and to a lesser extent by IL-2, reaching peak levels after 2-3 days of stimulation. Heat shock proteins are induced within minutes under stress conditions, via heat shock factors (HSF), which activate heat shock elements (HSE). IL-4, IL-2 and IL-13 upregulated transcription of a reporter gene coupled to a single HSE site and a minimal promoter. HSE may therefore be involved in cytokine induced heat shock gene transcription in the absence of cellular stress.
Metz
K
K
Theodor-Boveri-Institut für Biowissenchaften, Physiologische Chemie II, Würzburg, Germany.
Ezernieks
J
J
Sebald
W
W
Duschl
A
A
D001483:Base Sequence; D002478:Cells, Cultured; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D005786:Gene Expression Regulation; D017930:Genes, Reporter / Q000235:genetics; D018841:HSP90 Heat-Shock Proteins / Q000235:genetics*; D018869:Heat-Shock Response / Q000235:genetics*; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology; D015847:Interleukin-4 / Q000494:pharmacology*; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis; D013601:T-Lymphocytes; D014158:Transcription, Genetic / Q000502:physiology*; D014407:Tumor Cells, Cultured; D015854:Up-Regulation
D018841:HSP90 Heat-Shock Proteins; D007376:Interleukin-2; D012333:RNA, Messenger; D015847:Interleukin-4; D015500:Chloramphenicol O-Acetyltransferase