Phosphatidylcholine increases the secretion of triacylglycerol-rich lipoproteins by CaCo-2 cells.
The regulation of lipid synthesis and secretion by phosphatidylcholine was investigated in CaCo-2 cells grown on semi-permeable filters. In cells incubated with 1 mM taurocholate and 100-500 microM phosphatidylcholine, cholesteryl ester synthesis was decreased, triacylglycerol synthesis was increased modestly, whereas phospholipid synthesis was unaffected. Acyl-CoA-cholesterol acyltransferase activity was decreased secondary to a decrease in the substrate (cholesterol) supply. The basolateral secretion of newly synthesized triacyglycerol and triacyglycerol mass was significantly increased by phosphatidylcholine, whereas cellular triacylglycerol mass decreased. This effect was no specific for phosphatidylcholine as phosphatidylethanolamine and phosphatidylserine also increased the secretion of newly synthesized triacylglycerols. Dioleoylphosphatidylcholine was as effective as egg phosphatidylcholine in increasing triacylglycerol transport. Dipalmitoylphosphatidylcholine, in contrast, was without effect. Phosphatidylcholine also increased the basolateral secretion of apolipoprotein B (apoB) mass without altering apoB mRNA levels. Disruption of the Golgi apparatus by monensin or brefeldin A prevented the increase in apoB secretion by phosphatidylcholine. Compared with microsomes prepared from control cells, those from cells incubated with phosphatidylcholine contained more newly synthesized apoB. The percentage of new synthesized apoB isolated from teh lumen of microsomes (as an estimate of apoB destined for secretion), however, was similar in the two preparations. Thus in CaCo-2 cells incubated with phosphatidylcholine, the transport of apoB and triacylglycerols is increased whereas cholesteryl ester synthesis and secretion are decreased. A normally functioning secretory pathway is required for phosphatidylcholine to increase triacylglycerol-rich lipoprotein secretion.
Mathur
S N
SN
University of Iowa, Department of Internal Medicine, Iowa City, IA 52242, U.S.A.
Born
E
E
Murthy
S
S
Field
F J
FJ
D018938:Caco-2 Cells; D006801:Humans; D008074:Lipoproteins / Q000378:metabolism*; D010713:Phosphatidylcholines / Q000378:metabolism*; D014280:Triglycerides / Q000378:metabolism*
D008074:Lipoproteins; D010713:Phosphatidylcholines; D014280:Triglycerides