Identification of cholesta-3,5-dien-7-one by gas chromatography-mass spectrometry in the erythrocyte membrane of alcoholic patients.
Lipids and oxidized lipids were analyzed by gas chromatography-mass spectrometry in the erythrocyte membranes of alcoholic and control subjects. Cholesta-3,5-dien-7-one and cholesta-trienes were detected in alcoholic samples examined, but not in significant amounts in controls. Levels of polyunsaturated fatty acids (arachidonic acid, 20:4; docosahexaenoic acid, 22:6; and docosatetraenoic acid, 22:4) in alcoholic samples declined significantly, whereas cholesta-3,5-dien-7-one levels increased. A high level of total bilirubin was observed in most patients. A possible mechanism of the accumulation of cholesta-3,5-dien-7-one in the erythrocyte membrane of alcoholics is discussed.
Adachi
J
J
Department of Legal Medicine, Kobe University School of Medicine, Japan.
Hojo
K
K
Ueno
Y
Y
Naito
T
T
Ninomiya
I
I
Imamichi
H
H
Tatsuno
Y
Y
D000328:Adult; D000437:Alcoholism / Q000097:blood* / Q000534:rehabilitation; D001663:Bilirubin / Q000097:blood; D002775:Cholestadienols / Q000097:blood*; D004910:Erythrocyte Membrane / Q000378:metabolism*; D005230:Fatty Acids, Nonesterified / Q000097:blood; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D015227:Lipid Peroxidation / Q000502:physiology; D008111:Liver Function Tests; D008297:Male; D008875:Middle Aged
D002775:Cholestadienols; D005230:Fatty Acids, Nonesterified; C071069:cholesta-3,5-dien-7-one; D001663:Bilirubin