Improved graft survival in cadaveric renal retransplantation by flow crossmatching.
OBJECTIVE
To evaluate the role of flow cytometry cross-matching on graft survival in patients undergoing cadaveric renal retransplantation compared with our conventional antihuman globulin cytotoxic crossmatch.


METHODS
In 1990, 6 of 7 transplantation centers in 1 organ procurement organization service area began performing cadaveric renal retransplantation only if the flow T-cell IgG crossmatch was negative. During that period, 1 center continued to use only the antihuman globulin T-cell IgG crossmatch. Prior to 1990, all centers used only the antihuman globulin T-cell IgG crossmatch as their crossmatch selection criterion for retransplantation. Regraft survival was compared between those centers by crossmatch selection criteria.


METHODS
Patient selection and immunosuppression decisions were made at the transplantation center.


METHODS
All flow cytometry crossmatches for all 7 centers participating in the evaluation were performed at the Histocompatibility Laboratory of the Midwest Organ Bank Inc, Westwood, Kan.


RESULTS
Graft survival is significantly better (P = .03 [logrank test]) in regrafts when the flow crossmatch is used to select patients for transplantation.


CONCLUSIONS
Flow crossmatching improves graft survival in cadaveric renal retransplantation by identifying a subset of patients with donor-directed HLA class I antibodies that are not detectable by our conventional antihuman globulin crossmatch.
Nelson
P W
PW
Midwest Organ Bank Inc, Westwood, Kan, USA.
Eschliman
P
P
Shield
C F
CF
Aeder
M I
MI
Luger
A M
AM
Pierce
G E
GE
Bryan
C F
CF
D002102:Cadaver; D003627:Data Interpretation, Statistical; D005069:Evaluation Studies as Topic; D005260:Female; D005434:Flow Cytometry; D006085:Graft Survival; D006650:Histocompatibility Testing / Q000379:methods*; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D016030:Kidney Transplantation; D007959:Lymphocyte Culture Test, Mixed; D008297:Male; D008875:Middle Aged; D012086:Reoperation; D013601:T-Lymphocytes / Q000276:immunology
D007074:Immunoglobulin G