title: Use of peptide immunization for porcine insulin of a very high homology with a host protein.
abstract: An anti-peptide antibody was obtained against a peptide corresponding to the 12 amino acids of the C-terminal region of porcine insulin B chain. The adsorption characteristics of this antibody were compared with those of anti-porcine insulin and anti-sheep insulin antibodies. Immunization of rabbits using the peptide corresponding to the C-terminal region of porcine insulin B chain produced an anti-peptide antibody which reacted with native porcine insulin with a much higher association constant than that of an anti-porcine insulin antibody. In addition, this immunization technique did not cause any observable physiologically harmful effects, such as hypoglycemia, in the immunized rabbits. Thus, peptide immunization may be a useful strategy when target proteins have biological activity and/or toxicity, and also have a very high degree of homology with the corresponding proteins of the immunized animal, which may inhibit the production of antibodies with a high association constant.
authors.affiliation: Department of Synthetic Chemistry and Biological Chemistry, Kyoto University, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Katoh
authors.forename: M
authors.initials: M
authors.lastname: Terashima
authors.forename: K
authors.initials: K
authors.lastname: Tomioka
authors.forename: N
authors.initials: N
authors.lastname: Hikida
mesh_terms: D000327:Adsorption; D000595:Amino Acid Sequence; D000818:Animals; D000915:Antibody Affinity; D003429:Cross Reactions; D007114:Immunization; D007328:Insulin / Q000737:chemistry / Q000276:immunology*; D007330:Insulin Antibodies / Q000737:chemistry; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000276:immunology*; D011817:Rabbits; D051381:Rats; D017386:Sequence Homology, Amino Acid; D013552:Swine
chemical_list: D007328:Insulin; D007330:Insulin Antibodies; D010455:Peptides