Effect of chronic ethanol consumption on the activities of residual small bowel brush-border enzymes after proximal jejunum resection in the rat.
Ethanol consumption has a toxic effect on the epithelium of the small bowel, but enterocyte maturity is very difficult to measure under these circumstances. However, when ethanol intake is combined with enterectomy, enterocyte immaturity is greater, permitting an easier separation of these two effects. In a group of rats (13 male Wistar rats weighing approximately 220 g) fed a liquid diet containing 35% ethanol for 4 weeks after resection of the proximal jejunum, the residual small intestine brush border maltase, sucrase, and lactase activities were similar to those of a pair-fed control group (13 animals). However, alkaline phosphatase activity was decreased in the mucosa and in the enterocyte brush border, probably because of the lower activity of this enzyme in the jejunum-ileum remnant of the alcoholic group.
Zucoloto
S
S
Department of Pathology, University of São Paulo, Ribeirão Preto, Brazil.
Braulio
V B
VB
Santos
G C
GC
Ramalho
F S
FS
Scandar
M P
MP
de Freitas
O
O
de Oliveira
J A
JA
D000437:Alcoholism / Q000201:enzymology*; D000469:Alkaline Phosphatase / Q000378:metabolism*; D000818:Animals; D000431:Ethanol / Q000633:toxicity*; D007413:Intestinal Mucosa / Q000187:drug effects* / Q000201:enzymology; D007583:Jejunum / Q000601:surgery*; D043322:Lactase; D008297:Male; D008871:Microvilli / Q000187:drug effects* / Q000201:enzymology; D051381:Rats; D017208:Rats, Wistar; D013393:Sucrase / Q000378:metabolism*; D000520:alpha-Glucosidases / Q000378:metabolism*; D001616:beta-Galactosidase / Q000378:metabolism*
D000431:Ethanol; D000469:Alkaline Phosphatase; D043322:Lactase; D000520:alpha-Glucosidases; D001616:beta-Galactosidase; D013393:Sucrase