title: A 28-bp negative element with multiple factor-binding activity controls expression of the vimentin-encoding gene.
abstract: The promoter of the human vimentin-encoding gene (VIM) contains two enhancers separated by a negative region. The distal and proximal enhancers bind the transcription factors, AP-1 and NK-kappaB, respectively, which contribute to serum induction of Vim synthesis. We were interested in looking for particular regulatory elements that might be responsible for tissue-specific extinction and culture-dependent activation of human VIM. We have identified a 48-bp sequence in the distal enhancer which had not been reported before. This sequence includes a negative element, NE2, which confers transcriptional repression in transfection experiments and binds at least two factors in vitro. NE2 may participate in the differentiation-stage-specific control of VIM expression which involves multiple regulatory sequences and several positive and negative trans-acting factors.
authors.affiliation: Laboratoire de Biologie Moléculaire de la Différenciation, Université Paris, France.
authors.forename: V
authors.initials: V
authors.lastname: Moura-Neto
authors.forename: M H
authors.initials: MH
authors.lastname: Kryszke
authors.forename: Z
authors.initials: Z
authors.lastname: Li
authors.forename: P
authors.initials: P
authors.lastname: Vicart
authors.forename: A
authors.initials: A
authors.lastname: Lilienbaum
authors.forename: D
authors.initials: D
authors.lastname: Paulin
mesh_terms: D001482:Base Composition; D001483:Base Sequence; D001665:Binding Sites; D015500:Chloramphenicol O-Acetyltransferase / Q000096:biosynthesis; D017931:DNA Primers; D003850:Deoxyribonuclease I; D004742:Enhancer Elements, Genetic; D005786:Gene Expression Regulation; D006367:HeLa Cells; D006801:Humans; D015151:Immunoblotting; D008969:Molecular Sequence Data; D016328:NF-kappa B / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D012689:Sequence Homology, Nucleic Acid; D018808:Transcription Factor AP-1 / Q000378:metabolism; D014162:Transfection; D014746:Vimentin / Q000096:biosynthesis* / Q000235:genetics*
chemical_list: D017931:DNA Primers; D016328:NF-kappa B; D011993:Recombinant Fusion Proteins; D018808:Transcription Factor AP-1; D014746:Vimentin; D015500:Chloramphenicol O-Acetyltransferase; D003850:Deoxyribonuclease I