Pharmacological and molecular characterization of the neurotensin receptor expressed in Sf9 cells.
The rat neurotensin receptor was expressed in Spodoptera frugiperda insect (Sf9) cells using infection with a recombinant baculovirus. Immunoblot experiments performed with an antibody raised against the C-terminus of the receptor showed major bands at 47 (corresponding to the unglycosylated receptor protein) and 50 kDa, and minor bands at 65 and 36 kDa. The expressed receptor bound 125I-neurotensin with high affinity, was coupled to endogenous G-proteins, and agonist-induced inositol phosphate production was observed at early times after infection. These results show that the rat neurotensin receptor retains functional properties when expressed in the heterologous insect cell system.
Boudin
H
H
Institut National de la Santé et de la Recherche Médicale, Hôpital St Antoine, Paris, France.
Labrecque
J
J
Lhiaubet
A M
AM
Dennis
M
M
Rostène
W
W
Pélaprat
D
D
D000818:Animals; D016367:Baculoviridae / Q000235:genetics; D015153:Blotting, Western; D002460:Cell Line; D003001:Cloning, Molecular; D005822:Genetic Vectors; D007457:Iodine Radioisotopes; D009496:Neurotensin / Q000378:metabolism; D011485:Protein Binding; D051381:Rats; D018028:Receptors, Neurotensin / Q000235:genetics* / Q000378:metabolism; D018411:Spodoptera
D007457:Iodine Radioisotopes; D018028:Receptors, Neurotensin; D009496:Neurotensin