title: Real-time two-photon confocal microscopy using a femtosecond, amplified Ti:sapphire system.
abstract: The bilateral imaging approach known from confocal applications operating in the line mode was used to realize real-time two-photon imaging. It is shown that the sectioning inherent to two-photon imaging could be improved by the introduction of a confocal line aperture in the imaging path. Using a high-power, low-repetition-rate amplified Ti:sapphire system, various biological objects were visualized including live boar sperm.
authors.affiliation: Department of Molecular Cell Biology, University of Amsterdam, The Netherlands.
authors.forename: G J
authors.initials: GJ
authors.lastname: Brakenhoff
authors.forename: J
authors.initials: J
authors.lastname: Squier
authors.forename: T
authors.initials: T
authors.lastname: Norris
authors.forename: A C
authors.initials: AC
authors.lastname: Bliton
authors.forename: M H
authors.initials: MH
authors.lastname: Wade
authors.forename: B
authors.initials: B
authors.lastname: Athey
mesh_terms: D000818:Animals; D007834:Lasers; D008297:Male; D008433:Mathematics; D018613:Microscopy, Confocal / Q000295:instrumentation / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods*; D011058:Pollen / Q000166:cytology*; D013094:Spermatozoa / Q000166:cytology*; D013552:Swine