Induction of NO synthesis in macrophages by Newcastle disease virus is associated with activation of nuclear factor-kappa B.
Newcastle disease virus (NDV) has received much attention recently because of its non-specific immune stimulating potential and its various anti-tumor activities. Here we describe that NDV induces synthesis of NO and causes an activation of nuclear factor-kappa B (NF-kappa B) in murine macrophages. These reactions were part of an activation process which included also stimulation of adenosine deaminase and inhibition of 5'-nucleotidase. NDV-mediated NO synthesis and NF-kappa B activation were blocked by an antioxidant (butylated hydroxyanisole), by an inhibitor of protein tyrosine kinase (genistein) and of protein kinase A (H-89), but not by an inhibitor of protein kinase C (staurosporin). These data suggest that signalling requirements of NF-kappa B activation and NO production in NDV-treated macrophages are similar.
Umansky
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Tumor Immunology Program, German Cancer Research Center, Heidelberg, Germany.
Shatrov
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Lehmann
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Schirrmacher
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D000243:Adenosine Deaminase / Q000378:metabolism; D000818:Animals; D017868:Cyclic AMP-Dependent Protein Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism; D008262:Macrophage Activation; D008264:Macrophages / Q000201:enzymology / Q000378:metabolism* / Q000821:virology*; D051379:Mice; D008811:Mice, Inbred DBA; D009699:N-Glycosyl Hydrolases / Q000378:metabolism; D016328:NF-kappa B / Q000502:physiology*; D009521:Newcastle Disease / Q000276:immunology* / Q000378:metabolism*; D009522:Newcastle disease virus / Q000276:immunology*; D009569:Nitric Oxide / Q000378:metabolism*; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism; D015398:Signal Transduction; D013154:Spleen / Q000737:chemistry / Q000201:enzymology
D016328:NF-kappa B; D009569:Nitric Oxide; D011505:Protein-Tyrosine Kinases; D017868:Cyclic AMP-Dependent Protein Kinases; D009699:N-Glycosyl Hydrolases; C030681:purine nucleosidase; D000243:Adenosine Deaminase