title: Inactive allele-specific methylation and chromatin structure of the imprinted gene U2af1-rs1 on mouse chromosome 11.
abstract: The imprinted U2af1-rs1 gene that maps to mouse chromosome 11 is predominately expressed from the paternal allele. We examined the methylation of genomic sequences in and around the U2af1-rs1 locus to establish the extent of sequence modifications that accompanied the silencing of the maternal allele. The analysis of HapII or HhaI sites showed that the silent maternal allele was hypermethylated in a block of CpG sequences that covered more than 10 kb. By comparison, the expressed paternal allele was unmethylated from a CpG island upstream of the transcribed region through 2 kb. An analysis of DNaseI hypersensitivity of a putative promoter of U2af1-rs1 showed an open chromatin conformation only on the unmethylated, expressed paternal allele. These results suggest that allele-specific hypermethylation covering the gene and its upstream CpG island plays a role in maternal allele repression of U2af1-rs1, which is reflected in altered chromatin conformation of DNaseI hypersensitive sites.
authors.affiliation: Genome Science Laboratory, RIKEN Tsukuba Life Science Center, Institute of Physical and Chemical Research (RIKEN), 3-1-1 Koyadai, Tsukuba Ibaraki, 305, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Shibata
authors.forename: K
authors.initials: K
authors.lastname: Yoshino
authors.forename: S
authors.initials: S
authors.lastname: Sunahara
authors.forename: Y
authors.initials: Y
authors.lastname: Gondo
authors.forename: M
authors.initials: M
authors.lastname: Katsuki
authors.forename: T
authors.initials: T
authors.lastname: Ueda
authors.forename: M
authors.initials: M
authors.lastname: Kamiya
authors.forename: M
authors.initials: M
authors.lastname: Muramatsu
authors.forename: Y
authors.initials: Y
authors.lastname: Murakami
authors.forename: I
authors.initials: I
authors.lastname: Kalcheva
authors.forename: C
authors.initials: C
authors.lastname: Plass
authors.forename: V M
authors.initials: VM
authors.lastname: Chapman
authors.forename: Y
authors.initials: Y
authors.lastname: Hayashizaki
mesh_terms: D000483:Alleles; D000818:Animals; D002843:Chromatin / Q000235:genetics* / Q000648:ultrastructure; D002874:Chromosome Mapping; D018899:CpG Islands; D003433:Crosses, Genetic; D005260:Female; D005786:Gene Expression Regulation; D018392:Genomic Imprinting; D008297:Male; D008745:Methylation; D051379:Mice / Q000235:genetics*; D008810:Mice, Inbred C57BL; D009115:Muridae / Q000235:genetics; D009419:Nerve Tissue Proteins; D009687:Nuclear Proteins; D011506:Proteins / Q000235:genetics*; D012261:Ribonucleoproteins; D000072668:Splicing Factor U2AF
chemical_list: D002843:Chromatin; D009419:Nerve Tissue Proteins; D009687:Nuclear Proteins; D011506:Proteins; D012261:Ribonucleoproteins; D000072668:Splicing Factor U2AF; C000607495:Zrsr1 protein, mouse