cDNA cloning of IX/X-BP, a heterogeneous two-chain anticoagulant protein from snake venom.
IX/X-bp is an anticoagulant protein isolated from the venom of the habu snake (Trimeresurus flavoviridis). It is a heterogeneous two-chain protein linked by an interchain S-S bond. We prepared a cDNA library from the venom gland of the habu snake in the vector pSPORT1. cDNA clones containing the coding sequences for IX/X-bp were isolated and sequenced to determine the structure of the proprotein of IX/X-bp. All cDNA clones containing coding sequences of either chain of IX/X-bp consisted of the 5'-end noncoding bases, the first ATG codon, a typical signal peptide sequence that was immediately followed by mature protein sequence that corresponded to one of the chains, a stop codon, the 3'-end noncoding bases, a polyadenylation signal, and a poly(A)+ region. These data indicate that the gene for each chain of the two-chain protein is transcribed and translated separately.
Matsuzaki
R
R
Department of Biochemistry, Meiji College of Pharmacy, Yatocho, Tanashi, Tokyo, Japan.
Yoshiara
E
E
Yamada
M
M
Shima
K
K
Atoda
H
H
Morita
T
T
D000595:Amino Acid Sequence; D000925:Anticoagulants; D001483:Base Sequence; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics*; D003001:Cloning, Molecular; D003062:Codon; D003435:Crotalid Venoms; D018076:DNA, Complementary / Q000737:chemistry*; D004220:Disulfides; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D030162:Reptilian Proteins; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA
D000925:Anticoagulants; D002352:Carrier Proteins; D003062:Codon; D003435:Crotalid Venoms; D018076:DNA, Complementary; D004220:Disulfides; D046911:Macromolecular Substances; D030162:Reptilian Proteins; C070302:factor IX-factor X-binding protein, Crotalinae