title: Clustering and functional cooperation of Ng-CAM and axonin-1 in the substratum-contact area of growth cones.
abstract: Growth cones and neurites of chicken dorsal root ganglia neurons cultured on laminin, Ng-CAM, or axonin-1 exhibit substratum-dependent morphology and growth patterns which are accompanied by distinctive distributions of axonin-1 and Ng-CAM in the growth cone membrane. On either Ng-CAM or axonin-1 substratum, both Ng-CAM and axonin-1 were depleted from some areas of the apical growth cone membrane. In contrast, on laminin, both axonin-1 and Ng-CAM remained randomly distributed. Removal of axonin-1 from growth cones resulted in a blockage of neurite outgrowth on both Ng-CAM and axonin-1 substrata, indicating that in these neurons axonin-1 cooperates with Ng-CAM in the activation of axon growth. Based on these results possible molecular models for cooperation between axonin-1 and Ng-CAM on the growth cone are discussed.
authors.affiliation: Biochemisches Institut, Universität Zürich, Winterthurerstrasse 190, Zürich, CH-8057, Switzerland.
authors.forename: E T
authors.initials: ET
authors.lastname: Stoeckli
authors.forename: U
authors.initials: U
authors.lastname: Ziegler
authors.forename: A J
authors.initials: AJ
authors.lastname: Bleiker
authors.forename: P
authors.initials: P
authors.lastname: Groscurth
authors.forename: P
authors.initials: P
authors.lastname: Sonderegger
mesh_terms: D000818:Animals; D019138:Antibodies, Blocking; D001369:Axons / Q000502:physiology* / Q000648:ultrastructure; D019062:Cell Adhesion Molecules, Neuron-Glia / Q000032:analysis / Q000494:pharmacology / Q000502:physiology*; D015816:Cell Adhesion Molecules, Neuronal / Q000032:analysis / Q000494:pharmacology / Q000502:physiology*; D002478:Cells, Cultured; D002642:Chick Embryo; D058969:Contactin 2; D005455:Fluorescent Antibody Technique; D005727:Ganglia, Spinal / Q000166:cytology / Q000196:embryology; D007150:Immunohistochemistry; D008855:Microscopy, Electron, Scanning; D016253:Microscopy, Immunoelectron; D016501:Neurites / Q000737:chemistry / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure
chemical_list: D019138:Antibodies, Blocking; D019062:Cell Adhesion Molecules, Neuron-Glia; D015816:Cell Adhesion Molecules, Neuronal; D058969:Contactin 2