A high-resolution whole genome radiation hybrid map of human chromosome 17q22-q25.3 across the genes for GH and TK.
We have constructed a whole genome radiation hybrid (WG-RH) map across a region of human chromosome 17q, from growth hormone (GH) to thymidine kinase (TK). A panel of 128 WG-RH hybrid cell lines generated by X-irradiation and fusion has been tested for the retention of 39 sequence-tagged site (STS) markers by the polymerase chain reaction. This genome mapping technique has allowed the integration of existing VNTR and microsatellite markers with additional new markers and existing STS markers previously mapped to this region by other means. The WG-RH map includes eight expressed sequence tag (EST) and three anonymous markers developed for this study, together with 23 anonymous microsatellites and five existing ESTs. Analysis of these data resulted in a high-density comprehensive map across this region of the genome. A subset of these markers has been used to produce a framework map consisting of 20 loci ordered with odds greater than 1000:1. The markers are of sufficient density to build a YAC contig across this region based on marker content. We have developed sequence tags for both ends of a 2.1-Mb YAC and mapped these using the WG-RH panel, allowing a direct comparison of cRay6000 to physical distance.
Foster
J W
JW
Department of Genetics, University of Cambridge, United Kingdom. j_foster@mole.bio.cam.ac.uk
Schafer
A J
AJ
Critcher
R
R
Spillett
D J
DJ
Feakes
R W
RW
Walter
M A
MA
Dominguez-Steglich
M
M
Guioli
S
S
Brook
J D
JD
Goodfellow
P N
PN
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002874:Chromosome Mapping; D002886:Chromosomes, Human, Pair 17; D006224:Cricetinae; D017931:DNA Primers; D005819:Genetic Markers; D013006:Growth Hormone / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D016324:Sequence Tagged Sites; D013937:Thymidine Kinase / Q000235:genetics*
D017931:DNA Primers; D005819:Genetic Markers; D013006:Growth Hormone; D013937:Thymidine Kinase