Comparison of the metabolism of alcohols by rat hepatic and pulmonary alcohol dehydrogenase.
The metabolism of 1-butanol, 1-pentanol and 1-propanol by rat hepatic and pulmonary cytosolic preparations was measured with regard to ADH activity as influenced by pH and substrate concentration. Compared to lung, hepatic ADH activity showed little pH dependence with apparent Vmax values similar for the 3 alcohols. Apparent Km values were also similar and were lower than previously reported for ethanol. In contrast to the liver, little ADH activity was observed in pulmonary preparations at pH 7.2 or 9.0 with any alcohol. Pulmonary apparent Km values were considerably higher than those in the liver. Thus the optimum conditions for pulmonary ADH activity require an alkaline pH and high substrate concentrations.
Carlson
G P
GP
Department of Pharmacology and Toxicology School of Pharmacy and Pharmacal Sciences, Purdue University, West Lafayette, IN 47907-1334, USA.
Olson
R M
RM
D020001:1-Butanol; D000433:1-Propanol / Q000378:metabolism; D000426:Alcohol Dehydrogenase / Q000378:metabolism*; D000438:Alcohols / Q000378:metabolism*; D000818:Animals; D000440:Butanols / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008099:Liver / Q000201:enzymology* / Q000378:metabolism*; D008168:Lung / Q000201:enzymology* / Q000378:metabolism*; D008297:Male; D000439:Pentanols / Q000378:metabolism; D051381:Rats; D013379:Substrate Specificity
D000438:Alcohols; D000440:Butanols; D000439:Pentanols; D000431:Ethanol; D020001:1-Butanol; D000433:1-Propanol; D000426:Alcohol Dehydrogenase; C024999:n-pentanol