Selective activation of the proto-oncogene c-jun promoter by the transforming protein v-Rel.
The transcription factor v-Rel is a transforming protein of the reticuloendotheliosis virus. We found that v-Rel activates the promoter of the proto-oncogene c-jun. Two elements in the c-jun promoter were required for the activation by v-Rel. One was a kB-site (v-Rel binding site), and the other was a c-jun promoter region between -52 and +148 (c-jun promoter (-52/+148)). Two promoters with the kB-site(s), those of human immunodeficiency virus (HIV) and SV40, were not activated by v-Rel, but their kB-sites were activated when introduced upstream of the c-jun promoter (-52/+148). Thus, the c-jun promoter (-52/+148) had information for the selective activation of the c-jun promoter by v-Rel. v-Rel bound to the c-jun kB-site with the higher affinity than c-Rel, thereby activating the c-jun promoter more efficiently than c-Rel. Moreover, the activity of v-Rel mutants upon the c-jun promoter correlates with their transforming activity. Thus, the c-jun promoter activation by v-Rel may play a role in the transformation caused by v-Rel.
Fujii
M
M
Department of Molecular Virology and Oncology, Cancer Research Institute, Kanazawa University, Japan.
Minamino
T
T
Nomura
M
M
Miyamoto
K I
KI
Tanaka
J
J
Seiki
M
M
D000595:Amino Acid Sequence; D001483:Base Sequence; D002471:Cell Transformation, Neoplastic / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology*; D016758:Genes, jun; D006367:HeLa Cells; D006801:Humans; D008213:Lymphocyte Activation / Q000502:physiology; D008969:Molecular Sequence Data; D020572:Oncogene Proteins v-rel; D011401:Promoter Regions, Genetic / Q000502:physiology*; D000090063:Proto-Oncogene Mas; D016755:Proto-Oncogene Proteins c-jun / Q000096:biosynthesis / Q000235:genetics; D015682:Retroviridae Proteins, Oncogenic / Q000235:genetics / Q000502:physiology*; D018808:Transcription Factor AP-1 / Q000096:biosynthesis / Q000235:genetics; D014158:Transcription, Genetic
C000715470:MAS1 protein, human; D020572:Oncogene Proteins v-rel; D000090063:Proto-Oncogene Mas; D016755:Proto-Oncogene Proteins c-jun; D015682:Retroviridae Proteins, Oncogenic; D018808:Transcription Factor AP-1