title: Actions of acidic fibroblast growth factor fragments on food intake in rats.
abstract: Acidic fibroblast growth factor (aFGF) has suppressive effects on food intake. In the present study, the effect of aFGF fragments on food intake were investigated in rats. Infusion of a carboxyl-terminal fragment of aFGF, aFGF-(114-140), did not affect food intake, whereas an amino-terminal fragment of aFGF, aFGF-(1-15), was significantly inhibitory. Other amino-terminal fragments, aFGF-(1-20), aFGF-(1-29) and aFGF-(9-29), did not affect food intake. However, [Ala16]aFGF-(1-29) and [Ser16]aFGF-(1-29) in which the cysteine residue at position 16 was replaced with alanine and serine, respectively, had significant suppressive effects on food intake. Infusion of a functional antagonist for FGF receptor, anti-FGFR-1 antibody, into the lateral hypothalamus (LHA) significantly increased food intake. The results suggest that: the amino-terminal portion of aFGF is active in food intake suppression; the replacement of cysteine residue by alanine or serine is important in some amino-terminal aFGF fragments; and the LHA is involved in feeding suppression actions by aFGF and some fragments.
authors.affiliation: Division of Bio-Information Engineering, Faculty of Engineering, Toyama University, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Sasaki
authors.forename: Y
authors.initials: Y
authors.lastname: Oomura
authors.forename: A J
authors.initials: AJ
authors.lastname: Li
authors.forename: K
authors.initials: K
authors.lastname: Hanai
authors.forename: I
authors.initials: I
authors.lastname: Tooyama
authors.forename: H
authors.initials: H
authors.lastname: Kimura
authors.forename: N
authors.initials: N
authors.lastname: Yanaihara
authors.forename: T
authors.initials: T
authors.lastname: Hori
mesh_terms: D000409:Alanine; D000818:Animals; D000906:Antibodies / Q000008:administration & dosage / Q000494:pharmacology; D004435:Eating / Q000187:drug effects*; D016220:Fibroblast Growth Factor 1 / Q000737:chemistry / Q000494:pharmacology*; D007026:Hypothalamic Area, Lateral / Q000187:drug effects; D008297:Male; D010446:Peptide Fragments / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D017468:Receptors, Fibroblast Growth Factor / Q000037:antagonists & inhibitors / Q000276:immunology; D012694:Serine; D013329:Structure-Activity Relationship
chemical_list: D000906:Antibodies; D010446:Peptide Fragments; D017468:Receptors, Fibroblast Growth Factor; D016220:Fibroblast Growth Factor 1; D012694:Serine; D000409:Alanine