title: Construction of a CUP1 promoter-based vector to modulate gene expression in Saccharomyces cerevisiae.
abstract: We report the construction and characterization of a yeast shuttle-expression vector that can be used to express genes in yeast in a modulated form. This vector is centromeric, with a polylinker that optionally can provide an ATG start of translation. Expression features are based on the CUP1 yeast metallothionein gene promoter, which can be tightly modulated by copper.
authors.affiliation: Departamento de Biología Molecular de Plantas, UNAM, Cuernavaca, Morelos, Mexico.
authors.forename: J O
authors.initials: JO
authors.lastname: Mascorro-Gallardo
authors.forename: A A
authors.initials: AA
authors.lastname: Covarrubias
authors.forename: R
authors.initials: R
authors.lastname: Gaxiola
mesh_terms: D001483:Base Sequence; D002352:Carrier Proteins; D003001:Cloning, Molecular; D004271:DNA, Fungal; D015966:Gene Expression Regulation, Fungal; D005822:Genetic Vectors; D008668:Metallothionein / Q000235:genetics*; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics*
chemical_list: C500769:CUP1-1 protein, S cerevisiae; D002352:Carrier Proteins; D004271:DNA, Fungal; C007121:copper thionein; D008668:Metallothionein