The murine homolog of TB2/DP1, a gene of the familial adenomatous polyposis (FAP) locus.
The cDNA of the murine counterpart of the human TB2/DP1 (deleted in polyposis) gene, one of the six genes deleted in severe cases of familial adenomatous polyposis (FAP) disease, was isolated and analyzed. The murine transcript is 734-bp long and thereby considerably shorter than the 3100-bp human counterpart. This is due to a completely different 3' untranslated region in mouse which starts immediately after the translational stop codon, thereby deleting a RFLP (restriction-fragment length polymorphism) marker for this disease. The amino acid sequence, however, is 92% conserved between mouse and man. Triggering of murine mast cells by IgE plus antigen results in a decrease of TB2/DP1 mRNA up to 60% after 2 h implying a possible role of this gene in regulation of the allergic effector cell. Reverse transcription-polymerase chain reaction (RT-PCR) analysis shows an ubiquitous expression pattern in a number of mouse cell lines and tissues.
Prieschl
E E
EE
Department of Immunodermatology, Sandoz Research Institute, Vienna, Austria.
Pendl
G G
GG
Harrer
N E
NE
Baumruker
T
T
D011125:Adenomatous Polyposis Coli / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D004247:DNA; D015536:Down-Regulation; D015870:Gene Expression; D017491:Genes, APC; D006801:Humans; D007073:Immunoglobulin E; D008407:Mast Cells / Q000166:cytology; D051379:Mice; D008969:Molecular Sequence Data
D007073:Immunoglobulin E; D004247:DNA