title: A new set of versatile vectors for the heterologous expression of foreign genes using the baculovirus system.
abstract: A new set of versatile advanced baculovirus (BV) vectors for the production of fused proteins in insect cells, under the control of the strong polyhedrin promoter of the Autographa california nuclear polyhedrosis virus (AcMNPV), has been constructed. The vectors contain peptide tags which allow immunological detection, as well as purification of recombinant protein produced via the BV expression system.
authors.affiliation: Max-Planck-Institut für Biophysik, Abteilung Molekulare Membranbiologie, Frankfurt/Main, Germany.
authors.forename: T
authors.initials: T
authors.lastname: Lenhard
authors.forename: G
authors.initials: G
authors.lastname: Maul
authors.forename: W
authors.initials: W
authors.lastname: Haase
authors.lastname: Reiländer
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D004247:DNA; D015870:Gene Expression / Q000235:genetics*; D016259:Genes, myc; D005822:Genetic Vectors / Q000235:genetics*; D008555:Melitten / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D017924:Nucleopolyhedroviruses / Q000235:genetics*; D011498:Protein Precursors / Q000235:genetics; D021382:Protein Sorting Signals / Q000235:genetics; D011985:Receptors, Serotonin / Q000235:genetics; D016324:Sequence Tagged Sites; D018411:Spodoptera / Q000166:cytology
chemical_list: D011498:Protein Precursors; D021382:Protein Sorting Signals; D011985:Receptors, Serotonin; C078730:serotonin 5 receptor; D008555:Melitten; C016694:prepromelittin; D004247:DNA