Multicolor spectral karyotyping of human chromosomes.
The simultaneous and unequivocal discernment of all human chromosomes in different colors would be of significant clinical and biologic importance. Whole-genome scanning by spectral karyotyping allowed instantaneous visualization of defined emission spectra for each human chromosome after fluorescence in situ hybridization. By means of computer separation (classification) of spectra, spectrally overlapping chromosome-specific DNA probes could be resolved, and all human chromosomes were simultaneously identified.
Schröck
E
E
Diagnostic Development Branch, National Center for Human Genome Research, National Institutes of Health, Bethesda, MD 20892-4470, USA.
du Manoir
S
S
Veldman
T
T
Schoell
B
B
Wienberg
J
J
Ferguson-Smith
M A
MA
Ning
Y
Y
Ledbetter
D H
DH
Bar-Am
I
I
Soenksen
D
D
Garini
Y
Y
Ried
T
T
D000818:Animals; D001943:Breast Neoplasms / Q000235:genetics; D002869:Chromosome Aberrations; D002877:Chromosomes, Human / Q000648:ultrastructure*; D015342:DNA Probes; D005456:Fluorescent Dyes; D005583:Fourier Analysis; D006801:Humans; D006922:Hylobates / Q000235:genetics; D007091:Image Processing, Computer-Assisted; D017404:In Situ Hybridization, Fluorescence; D007368:Interferometry; D007621:Karyotyping / Q000379:methods*; D013057:Spectrum Analysis; D014178:Translocation, Genetic; D014407:Tumor Cells, Cultured
D015342:DNA Probes; D005456:Fluorescent Dyes