BstF5I, an unusual isoschizomer of FokI.
BstF5I, a new restriction endonuclease (ENase) from Bacillus stearothermophilus F5, has been discovered. This enzyme recognizes 5'-GGATG-3' and cleaves DNA, generating a 2-base 3'extension: 5'-GGATG NN[symbol: see text]-3' 3'-CCTAC[symbol: see text]NN-5' BstF5I is an isoschizomer of FokI and seems to be evolutionarily close to other nonpalindromic-recognizing ENases from thermophilic bacilli.
Abdurashitov
M A
MA
SibEnzyme, Novosibirsk, Russia. info@siben.nsk.su
Kileva
E V
EV
Shinkarenko
N M
NM
Shevchenko
A V
AV
Dedkov
V S
VS
Degtyarev
S Kh
SKh
D001483:Base Sequence; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism*; D001411:Geobacillus stearothermophilus / Q000201:enzymology; D008969:Molecular Sequence Data; D013379:Substrate Specificity
C035042:endodeoxyribonuclease FokI; D015252:Deoxyribonucleases, Type II Site-Specific