title: Quantal acetylcholine release induced by mediatophore transfection.
abstract: Mediatophore is a protein of approximately 200 kDa able to translocate acetylcholine in response to calcium. It was purified from the presynaptic plasma membranes of the electric organ nerve terminals. Mediatophore is a homooligomer of a 16-kDa subunit, homologous to the proteolipid of V-ATPase. Cells of the N18TG-2 neuronal line are not able to produce quantal acetylcholine release. We show here that transfection of N18TG-2 cells with a plasmid encoding the mediatophore subunit restored calcium-dependent release. The essential feature of such a release was its quantal nature, similar to what is observed in situ in cholinergic synapses from which mediatophore was purified.
authors.affiliation: Département Pharmacologie, C.M.U., CH-1211 Geneva, Switzerland.
authors.forename: J
authors.initials: J
authors.lastname: Falk-Vairant
authors.forename: P
authors.initials: P
authors.lastname: Corrèges
authors.forename: L
authors.initials: L
authors.lastname: Eder-Colli
authors.forename: N
authors.initials: N
authors.lastname: Salem
authors.forename: E
authors.initials: E
authors.lastname: Roulet
authors.forename: A
authors.initials: A
authors.lastname: Bloc
authors.forename: F
authors.initials: F
authors.lastname: Meunier
authors.forename: B
authors.initials: B
authors.lastname: Lesbats
authors.forename: F
authors.initials: F
authors.lastname: Loctin
authors.forename: M
authors.initials: M
authors.lastname: Synguelakis
authors.forename: M
authors.initials: M
authors.lastname: Israel
authors.forename: Y
authors.initials: Y
authors.lastname: Dunant
mesh_terms: D000109:Acetylcholine / Q000378:metabolism*; D000818:Animals; D002460:Cell Line; D002999:Clone Cells; D015870:Gene Expression; D007700:Kinetics; D046911:Macromolecular Substances; D009419:Nerve Tissue Proteins / Q000096:biosynthesis / Q000378:metabolism*; D009447:Neuroblastoma; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000378:metabolism; D014101:Torpedo; D014162:Transfection; D014407:Tumor Cells, Cultured
chemical_list: D046911:Macromolecular Substances; D009419:Nerve Tissue Proteins; D011994:Recombinant Proteins; C050299:mediatophore; D000109:Acetylcholine