title: Effects of ethanol metabolism on PKC activity in isolated rat hepatocytes.
abstract: Isolated rat hepatocytes were exposed to increasing concentrations of ethanol. During exposure of cells to ethanol a moderate but significant modification in the level of hepatic PKC c-isoforms has been observed. The ethanol-induced effect on liver protein kinase C was reversed by 4-methylpyrazole, an inhibitor of alcohol dehydrogenase, indicating that the conversion of ethanol to acetaldehyde may be involved in the enzyme inactivation. The involvement of the alcohol metabolite in PKC modifications was confirmed by the exposure of hepatocytes or partially purified liver enzyme to acetaldehyde concentrations of pathological interest.
authors.affiliation: Institute of General Pathology, University of Genoa, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Domenicotti
authors.forename: D
authors.initials: D
authors.lastname: Paola
authors.forename: A
authors.initials: A
authors.lastname: Lamedica
authors.forename: R
authors.initials: R
authors.lastname: Ricciarelli
authors.forename: E
authors.initials: E
authors.lastname: Chiarpotto
authors.forename: U M
authors.initials: UM
authors.lastname: Marinari
authors.forename: G
authors.initials: G
authors.lastname: Poli
authors.forename: E
authors.initials: E
authors.lastname: Melloni
authors.forename: M A
authors.initials: MA
authors.lastname: Pronzato
mesh_terms: D000079:Acetaldehyde / Q000494:pharmacology; D000426:Alcohol Dehydrogenase / Q000037:antagonists & inhibitors / Q000378:metabolism; D000818:Animals; D004791:Enzyme Inhibitors / Q000494:pharmacology; D000431:Ethanol / Q000378:metabolism* / Q000494:pharmacology; D000077604:Fomepizole; D015151:Immunoblotting; D007527:Isoenzymes / Q000037:antagonists & inhibitors; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism; D008297:Male; D011493:Protein Kinase C / Q000037:antagonists & inhibitors*; D011720:Pyrazoles / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar
chemical_list: D004791:Enzyme Inhibitors; D007527:Isoenzymes; D011720:Pyrazoles; D000431:Ethanol; D000077604:Fomepizole; D000426:Alcohol Dehydrogenase; D011493:Protein Kinase C; D000079:Acetaldehyde