Breast cancer cells cultured in a system of new design presecrete an extracellular matrix and proliferate within it without cell-cell adhesion.
Breast cancer cells, MCF-7 line, when cultured in microporous, hydrophobic polymer tubes, presecrete ribbon shaped, gauze-like extracellular matrices within which they then reside and proliferate without observable cell-cell adhesion and can do so in the complete absence of serum supplement in the culture media. This cell functioning is attributed to the direct access of oxygen to the cell attachment site.
Fishman
J H
JH
Strang Cancer Research Laboratory, The Rockefeller University, New York, 10021, USA.
González
A
A
Osborne
M P
MP
D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology*; D002448:Cell Adhesion; D002455:Cell Division; D016895:Culture Media, Serum-Free; D003584:Cytological Techniques / Q000295:instrumentation; D005109:Extracellular Matrix / Q000378:metabolism*; D006801:Humans; D014407:Tumor Cells, Cultured
D016895:Culture Media, Serum-Free