Cloning of Xenopus TFIIS and its expression in oocytes and early embryos.
The transcriptional factor TFIIS has been cloned from Xenopus laevis. The length of the cDNA is 1668bp and contains the complete open reading frame of 303 amino acids. Xenopus TFIIS has high homologies to its human and mouse counterparts. In Northern blot analyses, TFIIS mRNAs that consisted of four different sizes were expressed relatively highly from the early stages of Xenopus oogenesis. During oocyte maturation, the pattern of Xenopus TFIIS messages showed a transient peak of expression. TFIIS mRNA occurred maternally and its level increased in later stage embryos. These data suggest that TFIIS mRNA is expressed in a developmentally regulated way in Xenopus laevis.
Kugawa
F
F
Department of Biological Pharmaceutical Sciences, College of Pharmacy, Nihon University, Chiba, Japan.
Shinga
J
J
Imagawa
M
M
Arae
K
K
Nagano
M
M
Shibata
M
M
Shiokawa
K
K
Aoki
M
M
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D017124:Conserved Sequence; D017931:DNA Primers; D004330:Drosophila; D004625:Embryo, Nonmammalian / Q000502:physiology*; D005260:Female; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009865:Oocytes / Q000502:physiology*; D009866:Oogenesis; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis; D011994:Recombinant Proteins / Q000096:biosynthesis; D012441:Saccharomyces cerevisiae; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000096:biosynthesis*; D035165:Transcription Factors, General; D014158:Transcription, Genetic; D035602:Transcriptional Elongation Factors; D014982:Xenopus laevis; D016335:Zinc Fingers
D017931:DNA Primers; D012333:RNA, Messenger; D011994:Recombinant Proteins; D014157:Transcription Factors; D035165:Transcription Factors, General; D035602:Transcriptional Elongation Factors; C018648:transcription factor S-II