cDNA cloning and genomic structure of human bone morphogenetic protein-3B (BMP-3b).
BMP-3b (also termed GDF-10) is a novel BMP-3 related protein recently discovered in rat femur tissue by molecular cloning. In this paper, we have isolated cDNA and the gene for human BMP-3b and determined their structure. Cloned human BMP-3b cDNA with a size of 2632 base pairs encodes a 478 amino acid precursor protein sharing 83% identity with rat BMP-3b. The human BMP-3b gene is composed of 3 exons and spans approximately 13 kilobases of DNA. The 5' flanking region carries no typical TATA box but G+C rich sequences. Southern blot analysis revealed that the BMP-3b gene is situated in a single locus of chromosome 10. By Northern analysis, human BMP-3b transcripts were detected primarily in femur, brain, lung, skeletal muscle, pancreas and testis.
Hino
J
J
National Cardiovascular Center Research Institute, Fujishirodai, Suita, Osaka, Japan.
Takao
M
M
Takeshita
N
N
Konno
Y
Y
Nishizawa
T
T
Matsuo
H
H
Kangawa
K
K
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D055398:Bone Morphogenetic Protein 3; D019485:Bone Morphogenetic Proteins; D001842:Bone and Bones / Q000378:metabolism; D001921:Brain / Q000378:metabolism; D002874:Chromosome Mapping; D002879:Chromosomes, Human, Pair 10; D003001:Cloning, Molecular; D017931:DNA Primers; D018076:DNA, Complementary; D005091:Exons; D055413:Growth Differentiation Factor 10; D006133:Growth Substances / Q000096:biosynthesis* / Q000235:genetics*; D006801:Humans; D008168:Lung / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000378:metabolism; D010179:Pancreas / Q000378:metabolism; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D012045:Regulatory Sequences, Nucleic Acid; D015183:Restriction Mapping; D016385:TATA Box; D013737:Testis / Q000378:metabolism; D014158:Transcription, Genetic
C525757:BMP3 protein, human; C525759:Bmp3 protein, rat; D055398:Bone Morphogenetic Protein 3; D019485:Bone Morphogenetic Proteins; D017931:DNA Primers; D018076:DNA, Complementary; C525785:GDF10 protein, human; C525786:Gdf10 protein, rat; D055413:Growth Differentiation Factor 10; D006133:Growth Substances; D012333:RNA, Messenger