title: Nuclear encoding of a chloroplast RNA polymerase sigma subunit in a red alga.
abstract: A chloroplast RNA polymerase sigma factor is encoded by a nuclear gene, sigA, in the red alga Cyanidium caldarium RK-1. The encoded protein functions as an RNA polymerase sigma factor in vitro and it is localized to the chloroplast in vivo. SigA shows high sequence similarity to the sigma factors of cyanobacteria, which is indicative of the ancestral endosymbiotic event and subsequent transfer of the sigA gene to the nuclear genome.
authors.affiliation: Institute of Molecular and Cellular Biosciences, University of Tokyo, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Tanaka
authors.forename: K
authors.initials: K
authors.lastname: Oikawa
authors.forename: N
authors.initials: N
authors.lastname: Ohta
authors.forename: H
authors.initials: H
authors.lastname: Kuroiwa
authors.forename: T
authors.initials: T
authors.lastname: Kuroiwa
authors.forename: H
authors.initials: H
authors.lastname: Takahashi
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D002467:Cell Nucleus / Q000235:genetics; D002736:Chloroplasts / Q000201:enzymology* / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000378:metabolism; D000461:Rhodophyta / Q000201:enzymology / Q000235:genetics* / Q000648:ultrastructure; D016415:Sequence Alignment; D012808:Sigma Factor / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D011994:Recombinant Proteins; D012808:Sigma Factor; C081775:RNA polymerase sigma 70; D012321:DNA-Directed RNA Polymerases