cDNA cloning of a novel CYP3A from rat brain.
One full length cDNA clone, designated 3aH15, was isolated from a control male rat brain cDNA library. 3aH15 encoded a protein composed of 503 amino acid residues. The deduced amino acid sequence of 3aH15 was 92% identical to Cyp3a-13 and had a 68.4% to 76.5% homology with the other reported CYP3A sequences. Clone 3aH15 was thus named CYP3A9. No significant induction of the CYP3A9 expression in rat brain by dexamethasone was observed by Northern blot analysis. CYP3A9 cDNA was expressed in E. coli and the expressed P450 3A9 is active in the demethylation of erythromycin as well as benzphetamine.
Wang
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Department of Biochemistry and Molecular Biology, University of Texas Medical School, Houston, 77225, USA.
Kawashima
H
H
Strobel
H W
HW
D000595:Amino Acid Sequence; D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases; D001483:Base Sequence; D015152:Blotting, Northern; D001921:Brain / Q000201:enzymology*; D003001:Cloning, Molecular; D019392:Cytochrome P-450 CYP2E1; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D018076:DNA, Complementary; D004926:Escherichia coli / Q000235:genetics; D008297:Male; D006899:Mixed Function Oxygenases / Q000235:genetics*; D008969:Molecular Sequence Data; D010089:Oxidoreductases, N-Demethylating / Q000235:genetics*; D051381:Rats; D017207:Rats, Sprague-Dawley
D018076:DNA, Complementary; D003577:Cytochrome P-450 Enzyme System; D006899:Mixed Function Oxygenases; D019392:Cytochrome P-450 CYP2E1; D001189:Aryl Hydrocarbon Hydroxylases; C415842:Cyp3a9 protein, rat; D010089:Oxidoreductases, N-Demethylating