Differential display PCR reveals increased expression of 2',3'-cyclic nucleotide 3'-phosphodiesterase by lithium.
Differential display PCR was used to study the effects of lithium on gene expression. Four candidate genes were isolated and verified by Northern hybridization after 1 week treatment of C6 glioma cells with therapeutically relevant concentrations of LiCl (1 mM). Sequencing analysis revealed three previously unidentified cDNA fragments in addition to a sequence with 99% homology with the cDNA for 2',3'-cyclic nucleotide 3'-phosphodiesterase type II (CNPaseII). Since CNPaseII is important in myelinogenesis and possibly neuronal growth and repair, the present findings suggest that lithium treatment may regulate these processes.
Wang
J F
JF
Department of Psychiatry, McMaster University, Hamilton, Ont., Canada.
Young
L T
LT
D063309:2',3'-Cyclic Nucleotide 3'-Phosphodiesterase; D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases / Q000235:genetics*; D001483:Base Sequence; D002220:Carbamazepine / Q000494:pharmacology; D015870:Gene Expression / Q000187:drug effects*; D006801:Humans; D018021:Lithium Chloride / Q000494:pharmacology*; D008969:Molecular Sequence Data; D009457:Neuroglia / Q000187:drug effects / Q000378:metabolism; D009838:Oligodeoxyribonucleotides; D010727:Phosphoric Diester Hydrolases; D016133:Polymerase Chain Reaction; D012965:Sodium Chloride / Q000494:pharmacology; D014407:Tumor Cells, Cultured; D014635:Valproic Acid / Q000494:pharmacology
D009838:Oligodeoxyribonucleotides; D002220:Carbamazepine; D012965:Sodium Chloride; D014635:Valproic Acid; D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases; D010727:Phosphoric Diester Hydrolases; D063309:2',3'-Cyclic Nucleotide 3'-Phosphodiesterase; C571337:CNP protein, human; D018021:Lithium Chloride