Effects of the anti-gastric secretory drugs IT-066 and omeprazole mitogenic activities in the gastric juice of the rat.
BACKGROUND
Salivary epidermal growth factor (EGF) retains its biologic function in gastric juice and may play a physiologic role. Little is know, however, about the existence of mitogens other than EGF and the constitutional alterations of these factors in gastric juice by anti-secretagogues.


METHODS
The mitogenic activity was evaluated by measuring [3H]-thymidine incorporation, and the EGF contribution was determined by using a specific anti-rat EGF antibody. An H2-receptor antagonist (IT-066) and a proton pump inhibitor (omeprazole) were used to determine whether these drugs alter the relative composition of active mitogens in gastric juice.


RESULTS
Normal gastric juice significantly increased DNA synthesis. This activity was suppressed by antibody (87-88%). Both drugs increased EGF concentrations and activity dose-dependently IT-066 specifically increased total amount and activity of EGF. Approximately 50% of this activity was reduced by boiling or antibody.


CONCLUSIONS
The major mitogenic activity of normal rat gastric juice depends on EGF, and antisecretory drugs enhance the mitogenic activity by preserving and including intraluminal mitogens than EGF.
Kasugai
K
K
First Dept. of Internal Medicine, Nagoya City University Medical School, Japan.
Joh
T
T
Kataoka
H
H
Kato
T
T
Itoh
M
M
D000818:Animals; D000897:Anti-Ulcer Agents / Q000494:pharmacology*; D002460:Cell Line; D004261:DNA Replication / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004815:Epidermal Growth Factor / Q000378:metabolism; D005750:Gastric Juice / Q000187:drug effects* / Q000378:metabolism; D006133:Growth Substances / Q000378:metabolism*; D006635:Histamine H2 Antagonists / Q000494:pharmacology*; D007124:Immunoenzyme Techniques; D009853:Omeprazole / Q000494:pharmacology*; D010880:Piperidines / Q000494:pharmacology*; D011725:Pyridines / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley
D000897:Anti-Ulcer Agents; D006133:Growth Substances; D006635:Histamine H2 Antagonists; D010880:Piperidines; D011725:Pyridines; C063924:IT 066; D004815:Epidermal Growth Factor; D009853:Omeprazole