[Saltatory movements of the cytoplasmic granules in the cells of an ESK culture].
Saltatory movements of large (0.3-0.8 micron) granules in the cytoplasm of PK cells were described in norm and after nocodazole and sodium azide treatments. In untreated cells the length of single movements was up to 2 micron and even more (sometimes up to 5 micron). Nocodazole at a concentration of 0.2 micron and sodium azide at a concentration of 20 micron inhibited all rapid movements longer than 1.2 micron, but did not affect the frequency of movements shorter than 1 micron. The effect of nocodazole was reversible: the long rapid movements were seen resumed after its removal. A comparison of histograms (length versus frequency) of the rapid movements of granules in norm and after nocodazole and sodium azide treatments made it possible to conclude that real saltations (ATP and microtubule-dependent motions) are rapid translocations which exceed 1 micron.
Speranskaia
S R
SR
Votchal
M S
MS
Vorob'ev
I A
IA
D000818:Animals; D001386:Azides / Q000494:pharmacology; D002460:Cell Line; D002478:Cells, Cultured; D003594:Cytoplasmic Granules / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D003595:Cytoplasmic Streaming / Q000187:drug effects / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D004622:Embryo, Mammalian; D007668:Kidney / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D008858:Microscopy, Phase-Contrast; D009153:Mutagens / Q000494:pharmacology; D015739:Nocodazole / Q000494:pharmacology; D012815:Signal Processing, Computer-Assisted; D019810:Sodium Azide; D013552:Swine
D001386:Azides; D009153:Mutagens; D019810:Sodium Azide; D015739:Nocodazole
Sal'tatornye dvizheniia tsitoplazmaticheskikh granul v kletkakh kul'tury SPEV.