LFA-1-deficient mice show normal CTL responses to virus but fail to reject immunogenic tumor.
The leukocyte integrin LFA-1 (CD11a/CD18) plays an important role in lymphocyte recirculation and homotypic interactions. Leukocytes from mice lacking CD11a displayed defects in in vitro homotypic aggregation, in proliferation in mixed lymphocyte reactions, and in response to mitogen. Mutant mice mounted normal cytotoxic T cell (CTL) responses against systemic LCMV and VSV infections and showed normal ex vivo CTL function. However, LFA-1-deficient mice did not reject immunogenic tumors grafted into footpads and did not demonstrate priming response against tumor-specific antigen. Thus CD11a deficiency causes a selective defect in induction of peripheral immune responses whereas responses to systemic infection are normal.
Schmits
R
R
Amgen Institute, Ontario Cancer Institute, Department of Medical Biophysics, Toronto, Canada.
Kündig
T M
TM
Baker
D M
DM
Shumaker
G
G
Simard
J J
JJ
Duncan
G
G
Wakeham
A
A
Shahinian
A
A
van der Heiden
A
A
Bachmann
M F
MF
Ohashi
P S
PS
Mak
T W
TW
Hickstein
D D
DD
D000818:Animals; D001483:Base Sequence; D018821:CD18 Antigens / Q000235:genetics / Q000276:immunology*; D006720:Homozygote; D016023:Integrins / Q000235:genetics / Q000276:immunology*; D016169:Lymphocyte Function-Associated Antigen-1 / Q000235:genetics / Q000276:immunology*; D008217:Lymphocytic choriomeningitis virus / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D008969:Molecular Sequence Data; D016296:Mutagenesis; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014721:Vesicular stomatitis Indiana virus / Q000276:immunology; D014777:Virus Diseases / Q000276:immunology*
D018821:CD18 Antigens; D016023:Integrins; D016169:Lymphocyte Function-Associated Antigen-1