Effect of chlorophyllin on gamma ray induced micronuclei in polychromatic erythrocytes of murine peripheral blood determined by the ABC strategy.
The effect of chlorophyllin on micronucleated polychromatic erythrocyte (MN-PCE) induction by gamma ray exposure in peripheral blood of mice was studied. The area beneath the curve (ABC) of MN-PCE frequency versus time was used as an index of total MN-PCE induction. The dose of 200 mg chlorophYllin per kg of body weight caused a slight, but not significant, reduction of the MN-PCE caused by 1.0 Gy exposure. This result indicates that chlorophyllin did not protect the cells against MN induction. In previous studies it was observed that the same chlorophyllin dose was able to protect 100% against sister chromatid exchange (SCE) induction by 1.0 gamma rays in both murine spermatogonia and bone marrow cells. These contradictory results indicate that chlorophyllin did not protect cells by scavenging free radicals, but by other mechanism, i.e. stimulating repair of lesions involved in SCE induction.
Morales-Ramírez
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P
Departamento de Radiobiología, Instituto Nacional de Investigaciones Nucleares, Mexico, D.F.
Vallarino-Kelly
T
T
Rodríguez-Reyes
R
R
D000818:Animals; D016587:Antimutagenic Agents / Q000494:pharmacology*; D002735:Chlorophyllides / Q000494:pharmacology*; D004912:Erythrocytes / Q000528:radiation effects*; D016166:Free Radical Scavengers / Q000494:pharmacology; D005720:Gamma Rays; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D048629:Micronuclei, Chromosome-Defective / Q000187:drug effects / Q000528:radiation effects*
D016587:Antimutagenic Agents; D002735:Chlorophyllides; D016166:Free Radical Scavengers; C007020:chlorophyllin