A novel monoclonal antibody to N-myristoyl glycine moiety found a new N-myristoylated HIV-1 p28gag protein in HIV-1-infected cells.
A novel monoclonal antibody was raised against a synthetic N-myristoyl glycine that is characteristic of all N-myristoylated proteins. The immunoreaction suppressed in the presence of hemocyanin as well as albumin conjugated with N-myristoyl glycine and other N-myristoyl glycyl peptides, while underivatized and myristoyl amino acid proteins or various fatty acids other myristic acid exerted no effect. The antibody specifically reacted with N-myristoylated pp60c-src in human colon adenocarcinoma cells, N-myristoylated pp60v-src in Rous sarcoma virus-infected cells, and N-myristoylated Gag precursor protein Pr55gag in HIV-1 producing cells. Furthermore, the antibody immunoreacted with a new N-myristoylated p28gag derived from HIV-1 gag protein. The antibody is shown to be a very useful tool for identification of N-myristoylated proteins.
Furuishi
K
K
Department of Biochemistry, Faculty of Pharmaceutical Sciences, Kumamoto University, Japan.
Misumi
S
S
Shoji
S
S
D000230:Adenocarcinoma; D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D001358:Avian Sarcoma Viruses / Q000235:genetics; D002478:Cells, Cultured; D002642:Chick Embryo; D003110:Colonic Neoplasms; D004797:Enzyme-Linked Immunosorbent Assay; D005347:Fibroblasts; D015683:Gene Products, gag / Q000032:analysis / Q000096:biosynthesis* / Q000276:immunology / Q000378:metabolism; D005998:Glycine / Q000031:analogs & derivatives* / Q000032:analysis / Q000276:immunology; D015497:HIV-1 / Q000502:physiology*; D006801:Humans; D009227:Myristic Acids / Q000032:analysis* / Q000276:immunology; D011498:Protein Precursors / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D016392:Proto-Oncogene Proteins pp60(c-src) / Q000032:analysis / Q000276:immunology; D014779:Virus Replication
D000911:Antibodies, Monoclonal; D015683:Gene Products, gag; D009227:Myristic Acids; D011498:Protein Precursors; C059767:p55 gag precursor protein, Human immunodeficiency virus 1; C057393:N-myristoylglycine; D016392:Proto-Oncogene Proteins pp60(c-src); D005998:Glycine