Trafficking of major histocompatibility complex class II molecules through intracellular compartments containing HLA-DM.
The endosomal site(s) where MHC class II molecules become competent to bind antigenic peptide has not been completely characterized. We identified endocytic compartments through which newly synthesized MHC class II molecules move prior to their expression on the plasma membrane. The compartments co-sediment with lysosomes in the most dense regions of Percoll gradients. The appearance of proteolytic fragments of the invariant chain (I chain), namely leupeptin-induced proteins (LIPs) and class-II-associated invariant chain peptides (CLIP), in this region of the gradient suggests that the release of MHC class II molecules from I chain association occurs within these vesicles. The formation of SDS-stable alpha beta dimers indicated that MHC class II molecules contained within these compartments are receptive to peptide binding. A majority of the HLA-DM protein was found in the same region of the Percoll gradient, consistent with its established function in MHC class-II-restricted antigen presentation. Immunoelectron micrographs of dense-sedimenting compartments indicated that I chain, MHC class II, and DM molecules are contained within both multivesicular and multilamellar vesicles. The final stages of I chain dissociation from MHC class II molecules and DM-mediated peptide loading probably occur in these compartments.
Robbins
N F
NF
Section of Immunobiology, Howard Hughes Medical Institute, New Haven, CT 06510, USA.
Hammond
C
C
Denzin
L K
LK
Pan
M
M
Cresswell
P
P
D017951:Antigen Presentation; D000944:Antigens, Differentiation, B-Lymphocyte / Q000378:metabolism; D001402:B-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D001693:Biological Transport, Active / Q000276:immunology; D002451:Cell Compartmentation / Q000276:immunology*; D002461:Cell Line, Transformed; D002499:Centrifugation, Density Gradient; D006681:HLA-D Antigens / Q000378:metabolism*; D000949:Histocompatibility Antigens Class II / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D011205:Povidone; D012822:Silicon Dioxide; D013347:Subcellular Fractions / Q000276:immunology
D000944:Antigens, Differentiation, B-Lymphocyte; C416189:H2-M antigens; D006681:HLA-D Antigens; C084245:HLA-DM antigens; D000949:Histocompatibility Antigens Class II; C045084:invariant chain; C016039:Percoll; D012822:Silicon Dioxide; D011205:Povidone