An autonomously replicating eukaryotic expression vector with a tetracycline-responsive promoter.
The control elements of the tetracycline-resistance operon encoded in Tn10 of Escherichia coli have previously been utilized by Gossen and Bujard [ Proc. Natl. Acad. Sci. 89 (1992) 5547-5551] to establish an efficient transcription regulatory system in mammalian cells. We describe here a modification of this vector system that encodes all required elements, including a neomycin-resistance gene, on an autonomously replicating episome. This, thereby, allows for improved transfection frequency and episome stability, providing an easily selectable marker gene and a promoter whose activity can be easily modulated to control gene expression.
Lang
Z
Z
Bone Marrow Transplant Center, Rush University School of Medicine, Chicago, IL 60612, USA.
Feingold
J M
JM
D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D004261:DNA Replication; D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000235:genetics*; D005057:Eukaryotic Cells; D005786:Gene Expression Regulation; D005822:Genetic Vectors; D007668:Kidney; D008156:Luciferases / Q000096:biosynthesis; D008322:Mammals; D009876:Operon; D010957:Plasmids; D011401:Promoter Regions, Genetic / Q000187:drug effects; D011994:Recombinant Proteins / Q000096:biosynthesis; D015183:Restriction Mapping; D013752:Tetracycline / Q000494:pharmacology*; D014158:Transcription, Genetic
D004251:DNA Transposable Elements; D011994:Recombinant Proteins; D008156:Luciferases; D013752:Tetracycline