Purification and coat protein gene sequence of a Montana RMV-like isolate of barley yellow dwarf virus.
A Montana barley yellow dwarf virus (BYDV) isolate, BYDV-RMV-MT, is serologically identical to the New York RMV type isolate (RMV-NY) but differs in aphid transmission phenotype. A purification procedure for BYDV-RMV-MT was developed and cDNAs encompassing the entire coat protein gene and a portion of the putative polymerase gene of both RMV-MT and RMV-NY were cloned and sequenced. Diameters of RMV-MT virions averaged 24.7 nm. Average virus yield was 4.2 mg/kg plant tissue. There was 81% sequence identity between the clones of MT and NY RMV isolates at the nucleotide level. At the amino acid level the polymerase genes were 91% identical to each other and 74% homologous with that of beet western yellow virus. The coat protein amino acid sequences of the two RMV isolates were only 81% identical and, compared to other sequenced luteoviruses, both were most similar to cucurbit aphid-borne yellows virus.
Geske
S M
SM
Department of Plant Pathology, Montana State University-Bozeman, USA.
French
R
R
Robertson
N L
NL
Carroll
T W
TW
D000595:Amino Acid Sequence; D000818:Animals; D001042:Aphids; D001483:Base Sequence; D002213:Capsid / Q000235:genetics*; D003001:Cloning, Molecular; D017931:DNA Primers; D004279:DNA, Viral; D017793:Luteovirus / Q000235:genetics / Q000302:isolation & purification* / Q000648:ultrastructure; D008969:Molecular Sequence Data; D009009:Montana; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012367:RNA, Viral
D017931:DNA Primers; D004279:DNA, Viral; D012367:RNA, Viral