Role of type 1 fimbriae in the adhesion of Escherichia coli to salivary mucin and secretory immunoglobulin A.
Saliva is known to modulate the adhesion of bacteria in the oral cavity. The present work was performed to assess the effect of salivary components on the adhesion of Escherichia coli to a model oral surface. Several genetically engineered E. coli strains were used to examine the role of type 1 fimbriation in the interaction of these strains with salivary components in solution or adsorbed to hydroxyapatite. High (MG1) and low (MG2) molecular weight salivary mucins, and secretory immunoglobulin A (sIgA), were found to interact with the surface of E. coli, and these interactions were independent of the expression of fimbriae or capsule. In contrast, fimbriated strains of E. coli adhered to a greater extent to saliva-coated synthetic hydroxyapatite (HAP) than did nonfimbriated strains. Testing of salivary components separated by gel filtration chromatography revealed that only high-molecular-weight components promoted adhesion of E. coli to HAP. Additional studies found that purified MG2 and sIgA promoted the adhesion of E. coli to HAP. Expression of type 1 fimbriae enhanced adhesion, while mannose inhibited adhesion of fimbriated strains, to saliva-coated HAP and to HAP coated with MG2 and sIgA. We conclude that salivary MG2 and sIgA may provide receptors for the adhesion of type 1 fimbriated E. coli to oral surfaces.
Moshier
A
A
Department of Oral Biology, 318 Foster Hall, School of Dental Medicine, State University of New York at Buffalo, Buffalo, NY 14214, USA.
Reddy
M S
MS
Scannapieco
F A
FA
D001422:Bacterial Adhesion; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics / Q000502:physiology*; D010861:Fimbriae, Bacterial / Q000502:physiology*; D005818:Genetic Engineering; D006882:Hydroxyapatites; D015151:Immunoblotting; D007071:Immunoglobulin A, Secretory / Q000276:immunology*; D008358:Mannose / Q000494:pharmacology; D009055:Mouth / Q000276:immunology / Q000382:microbiology; D009077:Mucins / Q000037:antagonists & inhibitors / Q000502:physiology*; D012463:Saliva / Q000276:immunology* / Q000378:metabolism*
D006882:Hydroxyapatites; D007071:Immunoglobulin A, Secretory; D009077:Mucins; D008358:Mannose