title: Lepidoglyphus destructor acarus in the urban house environment.
abstract: To assess the presence of Lepidoglyphus destructor in the household environment of sensitized children living in an urban environment, samples of house dust were collected at the homes where two groups of patients were living, as well as in two bakeries in the city of Valencia, which were taken as a reference. Patients were divided into two groups. Group A included atopic children suffering from rhinitis and/or asthma, who were sensitized to L. destructor, as proven by prick test and specific IgE (CAP). Group B included children with the same features as those included in Group A, who were sensitized to Dermatophagoides pteronyssinus, with prick and CAP tests showing no significant sensitization to L. destructor. The samples of dust were analyzed, and the amounts of Der p I, Der f I, Der II and Lep d I per gram of dust were assessed through a solid-phase ELISA with monoclonal antibodies. In Group A, all patients but two showed a sensitization to D. pteronyssinus by prick test and serum IgE. At the homes of the patients from both groups, significant levels of Dermatophagoides were found. In Group A, only three houses showed levels of L. destructor which were comparable to those found in bakeries. Lep d I was not found in the houses of Group B patients. This means that a sensitization to L. destructor, as assessed with full extracts, is not always an indicator of its presence at the patient's house environment; it may rather refer to cross-reactivity to Dermatophagoides. Thus, availability of the main antigen Lep d I seems necessary to increase the specificity of the allergologic study.
authors.affiliation: Pediatrics Allergy Section, University General Hospital, University of Valencia, Spain.
authors.forename: J C
authors.initials: JC
authors.lastname: Juliá
authors.forename: A
authors.initials: A
authors.lastname: Martorell
authors.forename: P
authors.initials: P
authors.lastname: Ventas
authors.forename: J C
authors.initials: JC
authors.lastname: Cerdá
authors.forename: I
authors.initials: I
authors.lastname: Torró
authors.forename: J
authors.initials: J
authors.lastname: Carreira
authors.forename: E
authors.initials: E
authors.lastname: Guinot
authors.forename: J
authors.initials: J
authors.lastname: Sanz
authors.forename: V
authors.initials: V
authors.lastname: Alvarez
mesh_terms: D000293:Adolescent; D000485:Allergens / Q000032:analysis / Q000276:immunology*; D000818:Animals; D001249:Asthma / Q000209:etiology*; D002648:Child; D002675:Child, Preschool; D004391:Dust / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000175:diagnosis / Q000209:etiology / Q000276:immunology*; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology / Q000302:isolation & purification; D008297:Male; D008925:Mites / Q000276:immunology*; D012220:Rhinitis / Q000209:etiology*; D012882:Skin Tests; D014505:Urban Population
chemical_list: D000485:Allergens; D004391:Dust; D007073:Immunoglobulin E