Mitochondrial RNA editing is sequence specific and independent of transcript abundance in Sorghum bicolor.
The DNA sequence which encodes the amino-terminal extension to the conserved core of the atp6-1 gene found in line IS1112C is absent from Tx398. Sequences further upstream are present in Tx398, but at a different genomic location. The atp6-2 genes are present in similar copy numbers in IS1112C and A3Tx398, a near-isogenic line carrying the IS1112C cytoplasm in a Tx398 background. However, transcript abundance of atp6-2 in these lines is about ten-times higher than that of atp6-1. RNA editing of atp6-1 transcripts is identical to that of atp6-2 and therefore sequence specific. A single non-silent editing site in the unique atp6-1 pre-piece sequence may indicate mitochondrial-guided RNA editing. While in Petunia the abundance and RNA editing of a transcript are correlated, we show here that RNA editing is independent of transcript abundance and is sequence specific in Sorghum.
Kempken
F
F
Lehrstuhl für Allgemeine Botanik, Ruhr-Universität, D-44780 Bochum, Germany.
Howad
W
W
D018076:DNA, Complementary; D008928:Mitochondria / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics; D006109:Poaceae / Q000235:genetics*; D006180:Proton-Translocating ATPases; D012313:RNA / Q000378:metabolism*; D017393:RNA Editing; D012333:RNA, Messenger / Q000378:metabolism; D000077278:RNA, Mitochondrial
D018076:DNA, Complementary; D010940:Plant Proteins; D012333:RNA, Messenger; D000077278:RNA, Mitochondrial; D012313:RNA; D006180:Proton-Translocating ATPases