Sequence analysis of the 5'-flanking region of the gene encoding human N-acetylglucosaminyltransferase III.
We have isolated and characterized the immediate (1651 bP) 5'-flanking region of the gene (GnT-III) encoding N-acetylglucosaminyltransferase III (GnT-III) from a human placental genomic library. Analysis of promoter elements shows a similarity to the 5'-flanking region of murine 1,4-galactosyltransferase. The sequence lacks obvious TATA elements and CCAAT boxes; however, putative regulatory sites, including 2 potential cAMP-response regulatory elements (CRE), 11 insulin-response element consensus sequences (IRE), 7 potential AP-2 binding sites, 2 SP1 consensus sequences (GC boxes) and 2 sequences similar to the half-palindromic glucocorticoid-responsive element (GRE), are present.
Kim
Y J
YJ
Laboratory of Molecular and Cellular Biology, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Taejon, South Korea.
Park
J H
JH
Kim
K S
KS
Chang
J E
JE
Ko
J H
JH
Kim
M H
MH
Chung
D H
DH
Chung
T W
TW
Choe
I S
IS
Lee
Y C
YC
Kim
C H
CH
D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D004247:DNA; D006801:Humans; D008969:Molecular Sequence Data; D017351:N-Acetylglucosaminyltransferases / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012045:Regulatory Sequences, Nucleic Acid
D004247:DNA; D017351:N-Acetylglucosaminyltransferases; C054599:beta-1,4-mannosyl-glycoprotein beta-1,4-N-acetylglucosaminyltransferase