Structural characterization of a new galactofuranose-containing glycolipid antigen of Paracoccidioides brasiliensis.
An acidic glycolipid (Band 1), purified from P. brasiliensis by a combination of ion exchange chromatography, HPLC, and HPTLC, was found to be reactive with sera of all patients with paracoccidioidomycosis (PCM). Monosaccharide analysis of Band 1 yielded mannose and galactose in a 2:1 ratio, while mild acid hydrolysis and mild periodate oxidation/NaB3H4 reduction indicated the presence of a terminal galactofuranose. Preliminary analysis of 1H-NMR and MS data suggests that the structure of the glycan is Galf beta 1-->6(Manp alpha 1-->3)Manp beta 1-->2Ins (Ins = myo-inositol). Removal of the galacto-furanose decreased by 60-80% the reactivity of sera from PCM patients with Band 1, suggesting that this residue is immunodominant. With the presumed absence of galactofuranose in mammalian hosts, compounds containing this residue may be useful targets for therapy of several parasitic and fungal diseases.
Levery
S B
SB
Perkin-Elmer Applied Biosystems Division, Foster City, California 94404, USA.
Toledo
M S
MS
Suzuki
E
E
Salyan
M E
ME
Hakomori
S
S
Straus
A H
AH
Takahashi
H K
HK
D000946:Antigens, Fungal / Q000097:blood / Q000737:chemistry* / Q000302:isolation & purification; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D004797:Enzyme-Linked Immunosorbent Assay; D005690:Galactose / Q000032:analysis*; D006017:Glycolipids / Q000097:blood / Q000737:chemistry* / Q000302:isolation & purification; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry / Q000302:isolation & purification; D010228:Paracoccidioides / Q000737:chemistry*; D010229:Paracoccidioidomycosis / Q000097:blood* / Q000276:immunology; D016339:Spectrometry, Mass, Fast Atom Bombardment
D000946:Antigens, Fungal; D006017:Glycolipids; D009844:Oligosaccharides; D005690:Galactose