[A histopathological study of corneal amyloidosis secondary to trichiasis].
We present a case of secondary corneal amyloidosis whose etiological mechanism was investigated by immunohistochemistry and electron microscopy. A 48-year-old woman had suffered from trichiasis in the right eye for 35 years, and developed secondary corneal amyloidosis, a phenomenon previously described but whose etiological mechanism has not been explained. Slitlamp examination of the cornea revealed a white excrescence with a diameter of 2 mm. The lesion was excised and examined by light and electron microscopy. Large deposits of an amorphous eosinophilic material were observed beneath the atrophic epithelium. Amyloid was detected in these deposits using Congo red stain, polarized light, and electron microscopy. Neither vascularization nor infiltration of inflammatory cells was observed. Immunohistochemical tests for protein AL, protein AA, prealbumin, beta 2-microglobulin and cytokeratin in paraffin sections were all negative. Characteristic findings were observed in the border zone between the basal cells and the deposits. Numerous digitiform cell processes and membrane-bound globular fragments of basal cells were seen in the superficial region of the deposits. The cell membrane of some globules was interrupted and the contents appeared to have been discharged into the stroma. These findings suggest that basal cells of the corneal epithelium provide an amyloid precursor on the stroma.
Kigasawa
K
K
Department of Ophthalmology, Tokai University School of Medicine, Japan.
Mashima
Y
Y
Ogata
T
T
Tashiro
Y
Y
D000686:Amyloidosis / Q000209:etiology / Q000378:metabolism*; D003316:Corneal Diseases / Q000209:etiology / Q000378:metabolism*; D005140:Eyelashes / Q000002:abnormalities*; D005141:Eyelid Diseases / Q000150:complications; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged