The complete cDNA sequence encoding plasminogen from the European hedgehog (Erinaceus europaeus).
Using a PCR-based strategy, we have determined the complete cDNA sequence encoding hedgehog plasminogen (Plg). The 2700-nucleotide cDNA (corresponding to a 2.9-kb liver-derived transcript) encodes an open reading frame of 811 amino acids which shares 74-76% identity with Plg characterized from mouse, human and rhesus monkey. Residues corresponding to the catalytic triad, tPA-cleavage site, as well as seven of the eight lysine-binding residues in kringle IV are conserved in the hedgehog. However, potential N-linked glycosylation sites which have been reported in human and rhesus Plg are not present in analogous positions in the hedgehog Plg sequence.
Belczewski
A R
AR
Department of Biochemistry, Queen's University, Kingston, Ontario, Canada.
Laplaud
P M
PM
Chapman
M J
MJ
Koschinsky
M L
ML
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D015152:Blotting, Northern; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification; D005060:Europe; D006031:Glycosylation; D000882:Haplorhini; D006364:Hedgehogs / Q000235:genetics*; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D010958:Plasminogen / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D010958:Plasminogen