[Quantitative determination of the infective activity of the human immunodeficiency virus by plaque formation using monolayer cultures].
A method for assessment of the infective activity of HIV using plaque formation method with human diploid cells (strains L-65, L-68, L-72) has been developed. This method is not inferior to plaque formation with poly-L-lysine in sensitivity, but superior to it in reproducibility of results and standardization of the test conditions. The method is fit for titration of HIV and virus-neutralizing antibodies, as well as for assessment of the activity of anti-HIV preparations.
Nosik
M N
MN
Stepanova
L G
LG
Matsevich
G R
GR
D000914:Antibodies, Viral / Q000276:immunology; D002460:Cell Line; D006678:HIV / Q000276:immunology / Q000472:pathogenicity* / Q000502:physiology; D006801:Humans; D009500:Neutralization Tests; D015203:Reproducibility of Results; D010948:Viral Plaque Assay
D000914:Antibodies, Viral
Kolichestvennoe opredelenie infektsionnoĭ aktivnosti virusa immunodefitsita cheloveka metodom bliashkoobrazovaniia s ispol'zovaniem monosloĭnykh kul'tur.