title: Human endogenous retrovirus expression and reverse transcriptase activity in the T47D mammary carcinoma cell line.
abstract: We have examined the human mammary tumor cell line T47D and have found that these cells produce virus-like particles which band at the typical density for retroviral particles on a sucrose gradient, possess reverse transcriptase activity, and package HERV-K10-like sequences. Using this information and a bacterial expression system to identify long open reading frames, we have identified individual clones which have full-length open reading frames for reverse transcriptase and RNase H and which could encode the reverse transcriptase activity detected in these cells.
authors.affiliation: Chester Beatty Laboratories, Institute of Cancer Research, London, United Kingdom.
authors.forename: C
authors.initials: C
authors.lastname: Patience
authors.forename: G R
authors.initials: GR
authors.lastname: Simpson
authors.forename: A A
authors.initials: AA
authors.lastname: Colletta
authors.forename: H M
authors.initials: HM
authors.lastname: Welch
authors.forename: R A
authors.initials: RA
authors.lastname: Weiss
authors.forename: M T
authors.initials: MT
authors.lastname: Boyd
mesh_terms: D001483:Base Sequence; D004279:DNA, Viral; D005260:Female; D006801:Humans; D008969:Molecular Sequence Data; D012194:RNA-Directed DNA Polymerase / Q000032:analysis* / Q000235:genetics; D012190:Retroviridae / Q000201:enzymology / Q000302:isolation & purification*; D014407:Tumor Cells, Cultured / Q000821:virology*
chemical_list: D004279:DNA, Viral; D012194:RNA-Directed DNA Polymerase