Identification of a third thioredoxin gene from Corynebacterium nephridii.
We identified and sequenced a gene encoding a third thioredoxin (C3) from Corynebacterium nephridii. The determined nucleotide sequence encodes a thioredoxin of 145 amino acid residues, which is larger than most thioredoxins found in microbial cells and contains 6 cysteine residues. C. nephridii thioredoxin C3 is able to serve as a subunit of T7 DNA polymerase. C. nephridii is the first nonphotosynthetic procaryotic organism known to carry three different thioredoxins.
Lim
C J
CJ
Department of Biochemistry, Kangwon National University, Chuncheon, South Korea.
Sa
J H
JH
Fuchs
J A
JA
D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D001483:Base Sequence; D003001:Cloning, Molecular; D003352:Corynebacterium / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D013879:Thioredoxins / Q000737:chemistry* / Q000235:genetics*
D001426:Bacterial Proteins; D011994:Recombinant Proteins; C099780:thioredoxin C3 protein, Corynebacterium nephridii; D013879:Thioredoxins