Sequence of the mouse adenovirus type-1 DNA encoding the 100-kDa, 33-kDa and DNA-binding proteins.
The genomic nucleotide sequence for the region of 66 to 77 map units (m.u.) of mouse adenovirus type 1 (MAV-1) was determined and predicted to encode proteins homologous to the human adenovirus (Ad) 100-kDa, 33kDa and DNA-binding proteins (DBP). The putative MAV-1 100-kDa protein has 65-70% amino-acid similarity to 100-kDa proteins from five different human Ad serotypes. The mRNA for the putative 33-kDa protein is internally spliced within the coding sequence, as are its human Ad counterparts [Oosterom-Dragon and Anderson, J. Virol 45 (1983) 251-263]. The N-terminal region of the putative MAV-1 33-kDa protein has 41-44% similarity to two human Ad 33-kDa N-termini, and the C-terminal regions are more conserved, with 60-65% similarity. The MAV-1 DBP is predicted to be encoded in this region and was compared to six different human Ad DBP N-and C-termini. The N-termini of the MAV-1 and Ad DBP were 33-48% similar and the C-termini were 56-60% similar. The MAV-1 DBP contains conserved regions (CR) 1,2 and 3, and it retains important residues for a putative zinc finger (Zf) motif identified in Ad DBP [Eagle and Klessig, Virology 187 (1992) 777-787]. Additional sequence features of these proteins have also been identified.
Cauthen
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Department of Genetics, University of Georgia, Athens 30602-7223, USA.
Spindler
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KR
D000256:Adenoviridae / Q000235:genetics* / Q000378:metabolism; D000260:Adenoviruses, Human / Q000235:genetics / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017124:Conserved Sequence; D004279:DNA, Viral / Q000737:chemistry* / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010957:Plasmids; D017384:Sequence Deletion; D017386:Sequence Homology, Amino Acid
D004279:DNA, Viral; D004268:DNA-Binding Proteins