Cloning of a cDNA encoding a new ribosome-inactivating protein from Beta vulgaris vulgaris (mangold).
By means of a lambda ZAP II cDNA library constructed from seedings of Beta vulgaris vulgaris and immunoscreening, a cDNA clone containing a partial sequence of a new ribosome-inactivating protein (RIP) was obtained. As confirmed by Western blot analysis, this clone produced a RIP upon induction with IPTG. We called it betavulgin (Bvg). The recombinant protein (re-Bvg) was somewhat smaller than plant-derived RIP (28 versus 30 and 32 kDa), but showed the specific N-glycosidase activity on tobacco ribosomes, confirming its RIP character. The cDNA was sequenced and the missing 5'-end was established by RACE using bvg-specific primers. The entire cDNA was 1080 nucleotides in length and encoded a protein of 272 amino acids with a sequence identity of 26-40% with other RIP.
Hornung
E
E
Biological Institute, Department of Molecular Biology and Plant Virology, Stuttgart, Germany.
Wajant
H
H
Jeske
H
H
Mundry
K W
KW
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary; D018744:DNA, Plant; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism; D014675:Vegetables / Q000235:genetics* / Q000378:metabolism
C099900:Bvg protein, Beta vulgaris; D018076:DNA, Complementary; D018744:DNA, Plant; D010940:Plant Proteins