title: The cytomegalovirus antigenemia assay: a plea for standardization.
abstract: Its high diagnostic accuracy, rapidity, quantitative nature and technical simplicity have made the cytomegalovirus (CMV) antigenaemia assay one of the cornerstone methods for diagnosis and management of active CMV infection in immunocompromised patients. Many technical variations have been introduced in an effort to optimize the assay. Now, standardization of the assay and quality control are becoming of increasing importance. In this review we first discuss the nature and origin of the CMV antigens in distinct blood cells during active CMV infection. Further, some of the most important technical variations of the assay are considered and a proposal for standardization is made to indicate how quality control might be achieved. Acceptance of these proposals by the international community would be an important step forward, e.g. towards multicentre antigenaemia-directed intervention studies.
authors.affiliation: Department of Clinical Immunology, University Hospital, Groningen, The Netherlands.
authors.forename: T H
authors.initials: TH
authors.lastname: The
authors.forename: A P
authors.initials: AP
authors.lastname: van den Berg
authors.forename: M C
authors.initials: MC
authors.lastname: Harmsen
authors.forename: W
authors.initials: W
authors.lastname: van der Bij
authors.forename: W J
authors.initials: WJ
authors.lastname: van Son
mesh_terms: D000956:Antigens, Viral / Q000097:blood*; D003587:Cytomegalovirus / Q000276:immunology / Q000302:isolation & purification*; D003586:Cytomegalovirus Infections / Q000175:diagnosis* / Q000276:immunology; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D007124:Immunoenzyme Techniques; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000592:standards; D014763:Viral Matrix Proteins / Q000097:blood*
chemical_list: D000956:Antigens, Viral; D014763:Viral Matrix Proteins