Construction of gene expression system in cultured tobacco cells.
To construct a gene expression system in cultured tobacco cells, useful regulatory elements of plant genes were studied. The promoter of the horseradish peroxidase gene, prxC2, showed high activity in tobacco cells, and it contained enhancer sequences and a cis element for wound induction. The heat shock promoter of the HSP18.2 gene from A. thaliana had strong activity of transcription when the incubation temperature of tobacco cells was shifted from 25 degrees C to 37 degrees C. These elements could be good candidates for foreign gene expression in tobacco cells.
Shinmyo
A
A
Graduate School of Biological Science, Nara Institute of Science and Technology, Japan.
Yoshida
K
K
Kasai
T
T
D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D002455:Cell Division; D002478:Cells, Cultured; D004742:Enhancer Elements, Genetic; D015870:Gene Expression; D017343:Genes, Plant; D005966:Glucuronidase / Q000096:biosynthesis*; D006360:Heat-Shock Proteins / Q000096:biosynthesis; D006735:Horseradish Peroxidase / Q000096:biosynthesis*; D010947:Plants, Toxic; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000096:biosynthesis; D012045:Regulatory Sequences, Nucleic Acid; D016385:TATA Box; D014026:Nicotiana / Q000166:cytology / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism; D014162:Transfection / Q000379:methods*
D006360:Heat-Shock Proteins; D011994:Recombinant Proteins; D014157:Transcription Factors; D006735:Horseradish Peroxidase; D005966:Glucuronidase