Cloning and characterization of cDNAs coding for heavy and light chains of a monoclonal antibody (MabB23) specific for human plasma apolipoprotein B-100.
We have determined the nucleotide sequences encoding the heavy and light chains of the Fab fragment of murine monoclonal antibody MabB23(gamma2b,lambda), which is specific for human plasma apolipoprotein B-100 of low-density lipoproteins. The sequence analyses revealed that the variable regions of the heavy and light chains are members of mouse heavy-chain subgroup I(B) and lambda light-chain, respectively. A few unusual amino acids in the framework and constant regions of the heavy-chain were also noticed.
Kwak
J W
JW
Korea Research Institute of Bioscience and Biotechnology, KIST, Taejon, South Korea. jwkwak@geri4680.geri.ve.kr
Choi
B K
BK
Lee
D I
DI
Kang
Y K
YK
Seo
Y G
YG
Cho
W K
WK
Han
M H
MH
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000235:genetics* / Q000276:immunology; D058846:Antibodies, Monoclonal, Murine-Derived; D053299:Apolipoprotein B-100; D001055:Apolipoproteins B / Q000276:immunology*; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000235:genetics*; D007143:Immunoglobulin Heavy Chains / Q000737:chemistry / Q000235:genetics*; D007147:Immunoglobulin Light Chains / Q000737:chemistry / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D017421:Sequence Analysis; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D058846:Antibodies, Monoclonal, Murine-Derived; D053299:Apolipoprotein B-100; D001055:Apolipoproteins B; D018076:DNA, Complementary; D007140:Immunoglobulin Fab Fragments; D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; C099704:heavy chain, monoclonal antibody B23; C099703:light chain, monoclonal antibody B23