Assessment of the relationship between standard probe and implantable fiber measurements of cortical bone blood flow: a canine study.
Laser Doppler flowmetry (LDF) has been used to assess cortical bone blood flow in various clinical situations, such as osteomyelitis and osteonecrosis. Standard metal-sheathed probes containing optical fibers, applied to cortical bone for perfusion measurements, require direct exposure of the bone surface for each measurement, making nonanesthetized assessments over time impractical. Implantable optical fibers offer a noninvasive method for evaluating cortical bone perfusion without repeated surgical exposure of the bone after initial surgical implantation of the fibers. In vitro studies have shown the reliability of laser Doppler (LD) fibers compared with those of the standard probe. This investigation studied the relationship between measurements of cortical bone perfusion obtained by implanted optical (LD) fibers and standard (LDF) probes in vivo. Midshaft tibial fractures were created in the right hindlimb of 11 adult, large (>25 kg) dogs and stabilized by low contact-dynamic compression plate fixation. Cortical bone blood flow was measured by LDF using standard probes and implantable fibers at five sites along the tibia prefracture, postfracture, immediately postplate application, and at 10 weeks postplating, immediately prior to euthanasia. The implantable fibers were secured onto the cortical bone via the plate and led through a percutaneous exit site. Histological examination of the inguinal and popliteal lymph nodes and soft tissue surrounding the fibers revealed mild inflammation. No significant correlation of blood flow assessed by the implantable fibers and standard probe occurred immediately postfracture (r < 0. 13, p > 0.62). However, a statistically significant correlation was seen postplate application at one of the measurement sites in the distal fracture fragment (r = 0.78, P < 0.003). The fibers remained intact and functional until an average of 3 weeks at which time they either fractured or were removed by the animals. This is the first in vivo study assessing the reliability of implantable fibers for the measurement of cortical bone blood flow. Further modification of the fibers will be necessary to improve their longevity and durability for assessment of cortical bone blood flow.
Jain
R
R
Department of Surgery, St. Michael's Hospital, University of Toronto, Toronto, Ontario, Canada.
Podworny
N
N
Anderson
G I
GI
Schemitsch
E H
EH
D000818:Animals; D004285:Dogs; D005336:Fiber Optic Technology; D017078:Laser-Doppler Flowmetry / Q000295:instrumentation*; D055100:Optical Fibers; D019736:Prostheses and Implants; D012039:Regional Blood Flow; D013977:Tibia / Q000098:blood supply*