Cloning and chromosomal localization of a mouse cDNA with homology to the Saccharomyces cerevisiae gene zuotin.
The eukaryotic DnaJ homologs form a family of proteins with diverse functions. One member of the family, the Saccharomyces cerevisiae gene zuotin, was isolated for its ability to bind Z-DNA. Here, we have isolated a mouse cDNA called ZRF1 (for zuotin-related factor1) with significant homology to zuotin. The DnaJ domain and candidate phosphorylation sites of zuotin and ZRF1 are highly conserved. ZRF1 gene is localized on chromosome 5. The structural similarity of zuotin and ZRF1 suggests conservation of function of this DnaJ subfamily.
Hughes
R
R
Division of Hematology/Oncology, Howard Hughes Medical Institute, Children's Hospital, Boston, Massachusetts 02115, USA.
Chan
F Y
FY
White
R A
RA
Zon
L I
LI
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D016384:Consensus Sequence; D017124:Conserved Sequence; D004247:DNA / Q000378:metabolism; D018076:DNA, Complementary; D004268:DNA-Binding Proteins / Q000235:genetics*; D005656:Fungal Proteins / Q000235:genetics*; D005800:Genes, Fungal; D008040:Genetic Linkage; D050956:HSP40 Heat-Shock Proteins; D006360:Heat-Shock Proteins / Q000235:genetics; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D018832:Molecular Chaperones; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010766:Phosphorylation; D012150:Polymorphism, Restriction Fragment Length; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics*; D016601:RNA-Binding Proteins; D012091:Repetitive Sequences, Nucleic Acid; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic
D018076:DNA, Complementary; D004268:DNA-Binding Proteins; C097474:Dnajc2 protein, mouse; D005656:Fungal Proteins; D050956:HSP40 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D018832:Molecular Chaperones; D011506:Proteins; D016601:RNA-Binding Proteins; D029701:Saccharomyces cerevisiae Proteins; C077019:ZUO1 protein, S cerevisiae; D004247:DNA