An immunoglobulin E-dependent recombinant histamine-releasing factor induces interleukin-4 secretion from human basophils.
A novel recombinant histamine-releasing factor (rHFR), which stimulates secretion from a subpopulation of human basophils that express a particular type of immunoglobulin E (IgE) or IgE+, was found to induce interleukin-4 (IL-4) production from cells isolated from these same donors. The secretion of IL-4 protein induced by rHRF significantly correlated with histamine release and the amount of protein generated, and the kinetics were identical to those caused by anti-IgE activation. Furthermore, the ability of rHRF to induce IL-4 protein production from cells not normally responsive to this protein was transferred by passive sensitization with plasma containing IgE+ antibody. That this novel protein stimulates both mediator release and the secretion of IL-4 protein from human basophils suggests a prominent role for this molecule in allergic disease.
Schroeder
J T
JT
The Johns Hopkins Asthma and Allergy Center, Department of Medicine, Baltimore, Maryland 22124, USA.
Lichtenstein
L M
LM
MacDonald
S M
SM
D001491:Basophils / Q000187:drug effects / Q000276:immunology*; D014408:Biomarkers, Tumor; D002478:Cells, Cultured; D018932:Chemokine CCL2 / Q000494:pharmacology; D003001:Cloning, Molecular; D018076:DNA, Complementary; D006636:Histamine Release / Q000187:drug effects; D006801:Humans; D007116:Immunization, Passive; D007073:Immunoglobulin E / Q000096:biosynthesis*; D007377:Interleukin-3 / Q000494:pharmacology; D015847:Interleukin-4 / Q000096:biosynthesis*; D007700:Kinetics; D008222:Lymphokines / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000494:pharmacology; D000090324:Tumor Protein, Translationally-Controlled 1
D014408:Biomarkers, Tumor; D018932:Chemokine CCL2; D018076:DNA, Complementary; D007377:Interleukin-3; D008222:Lymphokines; D011994:Recombinant Proteins; D000090324:Tumor Protein, Translationally-Controlled 1; D015847:Interleukin-4; D007073:Immunoglobulin E