title: BstF5I, an unusual isoschizomer of FokI.
abstract: BstF5I, a new restriction endonuclease (ENase) from Bacillus stearothermophilus F5, has been discovered. This enzyme recognizes 5'-GGATG-3' and cleaves DNA, generating a 2-base 3'extension: 5'-GGATG NN[symbol: see text]-3' 3'-CCTAC[symbol: see text]NN-5' BstF5I is an isoschizomer of FokI and seems to be evolutionarily close to other nonpalindromic-recognizing ENases from thermophilic bacilli.
authors.affiliation: SibEnzyme, Novosibirsk, Russia. info@siben.nsk.su
authors.forename: M A
authors.initials: MA
authors.lastname: Abdurashitov
authors.forename: E V
authors.initials: EV
authors.lastname: Kileva
authors.forename: N M
authors.initials: NM
authors.lastname: Shinkarenko
authors.forename: A V
authors.initials: AV
authors.lastname: Shevchenko
authors.forename: V S
authors.initials: VS
authors.lastname: Dedkov
authors.forename: S Kh
authors.initials: SKh
authors.lastname: Degtyarev
mesh_terms: D001483:Base Sequence; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism*; D001411:Geobacillus stearothermophilus / Q000201:enzymology; D008969:Molecular Sequence Data; D013379:Substrate Specificity
chemical_list: C035042:endodeoxyribonuclease FokI; D015252:Deoxyribonucleases, Type II Site-Specific