title: Spectrophotometric assay for processes involving changes in hydrogen ion concentration in aqueous solution.
abstract: A strategy for the design of simple colorimetric assays to follow any process that involves the net generation or consumption of hydrogen ions in aqueous solutions over the pH range of 3-10 is described. This procedure relies upon the measurement of the change in absorption when a weakly or moderately buffered solution of a pH indicator is subjected to a small change in pH. Buffers and indicators are chosen with closely matching pKa values. The versatility of this type of assay technique is illustrated using three examples.
authors.affiliation: Department of Pharmacology and Developmental Therapeutics Program, Yale University School of Medicine, New Haven, Connecticut, 06520, USA.
authors.forename: P G
authors.initials: PG
authors.lastname: Penketh
authors.forename: K
authors.initials: K
authors.lastname: Shyam
authors.forename: C L
authors.initials: CL
authors.lastname: Patton
authors.forename: A C
authors.initials: AC
authors.lastname: Sartorelli
mesh_terms: D000477:Alkylating Agents / Q000737:chemistry; D000818:Animals; D002021:Buffers; D003124:Colorimetry / Q000379:methods; D005978:Glutathione / Q000737:chemistry; D006846:Hydrocarbons, Halogenated / Q000737:chemistry; D011522:Protons; D012996:Solutions; D013053:Spectrophotometry; D014346:Trypanosoma brucei brucei / Q000378:metabolism; D014867:Water / Q000737:chemistry*
chemical_list: D000477:Alkylating Agents; D002021:Buffers; D006846:Hydrocarbons, Halogenated; D011522:Protons; D012996:Solutions; D014867:Water; D005978:Glutathione