Characterization of the major citrate synthase of Bacillus subtilis.
The major citrate synthase of Bacillus subtilis (CS-II) was purified to near homogeneity and shown to correspond to the product of the citZ gene. Accumulation of CS-II during exponential growth and stationary phases paralleled expression of the citZ gene. The physical and kinetic properties of CS-II were similar to those of citrate synthase enzymes from Bacillus megaterium and from eukaryotic cells but differed from those of citrate synthases from many gram-negative bacteria.
Jin
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Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts 02111, USA.
Sonenshein
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D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000201:enzymology*; D015153:Blotting, Western; D002950:Citrate (si)-Synthase / Q000276:immunology / Q000378:metabolism*; D007700:Kinetics; D008969:Molecular Sequence Data; D008970:Molecular Weight
D002950:Citrate (si)-Synthase