Binding of Acanthamoeba to hydrogel contact lenses.
The numbers of Acanthamoeba binding to new hydrogel contact lenses of different polymer and water content were determined with two quantitative methods, a radiolabeled-cell method and a detaching-fluid method. Numbers of amoebae retained on nonionic lenses increased with increasing water content of the lenses. With both nonionic and ionic lenses numbers of associated amoebae decreased with successive rinsing steps. The retentions of amoebae on unworn hydrogel lenses, in contrast to the irreversible adhesion of bacteria, were tenuous and appeared to be effected mainly by surface tension, surface charge and water content.
Gorlin
A I
AI
Department of Biology, Georgia State University, Atlanta 30302-4010, USA.
Gabriel
M M
MM
Wilson
L A
LA
Ahearn
D G
DG
D000048:Acanthamoeba; D000818:Animals; D018441:Biofilms; D018639:Contact Lens Solutions; D003261:Contact Lenses; D004866:Equipment Contamination; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D007101:Immersion; D011092:Polyethylene Glycols / Q000737:chemistry; D014867:Water / Q000032:analysis
D018639:Contact Lens Solutions; D014867:Water; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D011092:Polyethylene Glycols