[A comparison of some methods for administering the Ca-dependent photoprotein obelin into the adipocytes of the brown fat in the rat].
An attempt was taken to incorporate Ca2(+)-dependent photoprotein obelin into young brown adipocytes using the three methods: 1) osmotic lysis of pinocytotic vesicles; 2) electroporation in a high electric field; 3) hypoosmotic shock. The young adipocytes were isolated from rat interscapular brown fat. The maximum incorporation of obelin into these cells was achieved using the hypoosmotic shock technique. A constant luminescence of intact cells loaded with obelin following hypoosmotic shock was observed in three independent experiments.
Elsukova
E I
EI
Medvedev
L N
LN
Luzhnov
Iu P
IuP
D017667:Adipocytes / Q000166:cytology / Q000187:drug effects*; D002001:Adipose Tissue, Brown / Q000166:cytology / Q000187:drug effects*; D000818:Animals; D018274:Electroporation / Q000379:methods; D005260:Female; D008163:Luminescent Measurements; D008164:Luminescent Proteins / Q000008:administration & dosage*; D009995:Osmosis / Q000187:drug effects; D009997:Osmotic Pressure / Q000187:drug effects; D010873:Pinocytosis / Q000187:drug effects; D051381:Rats
D008164:Luminescent Proteins; C010497:obelin
Sravnenie neskol'kikh metodov vvedeniia Ca-zavisimogo fotobelka obelina v adipotsity burogo zhira krysy.