Inverse polymerase chain reaction for cloning complete human immunoglobulin variable regions and leaders conserving the original sequence.
Perfetti
V
V
Research Laboratory of Biotechnology, University Hospital-I.R.C.C.S. Policlinico S. Matteo, P. le Golgi 2, Pavia, Italy.
Sassano
M
M
Ubbiali
P
P
Vignarelli
M C
MC
Arbustini
E
E
Corti
A
A
Merlini
G
G
D000686:Amyloidosis / Q000276:immunology; D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods*; D017124:Conserved Sequence; D017931:DNA Primers; D004587:Electrophoresis, Agar Gel; D006801:Humans; D007135:Immunoglobulin Variable Region / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*
D017931:DNA Primers; D007135:Immunoglobulin Variable Region