Spontaneous and induced synthesis of cytokines by peripheral blood monocytes in patients with endometriosis.
OBJECTIVE
To investigate the capacity of peripheral blood monocytes (PBM) from women with endometriosis to secrete tumor necrosis factor-alpha (TNF-alpha), interleukin (IL) IL-6, IL-8, and IL-10.


METHODS
Peripheral blood monocytes were cultured in the presence and absence of lipopolysaccharide for 24 hours before assessment of cytokines in supernatants by enzyme immunoassay.


METHODS
Institute for the Study and Treatment of Endometriosis and university-based research laboratories.


METHODS
Fertile controls, n - 10; women with endometriosis, n = 20.


METHODS
None.


RESULTS
Basal synthesis of TNF-alpha, IL-6, and IL-8 and the lipopolysaccharide-stimulated synthesis of TNF-alpha by PBM from women with endometriosis was significantly greater in comparison to that of fertile controls. Endometriosis had no effect on the basal or stimulated synthesis of IL-10.


CONCLUSIONS
These data show that endometriosis is associated with increased basal and stimulated synthesis and secretion of several different cytokines by PBM. Each of the cytokines found to be affected has the capacity to play a role in the symptomatology or pathogenesis of the disease.
Braun
D P
DP
Institute for the Study and Treatment of Endometriosis, Rush Medical College, Chicago, Illinois 60612, USA.
Gebel
H
H
House
R
R
Rana
N
N
Dmowski
N P
NP
D016207:Cytokines / Q000096:biosynthesis*; D004715:Endometriosis / Q000097:blood*; D005260:Female; D006801:Humans; D016753:Interleukin-10 / Q000096:biosynthesis; D015850:Interleukin-6 / Q000096:biosynthesis; D016209:Interleukin-8 / Q000096:biosynthesis; D008070:Lipopolysaccharides / Q000494:pharmacology; D009000:Monocytes / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D016207:Cytokines; D015850:Interleukin-6; D016209:Interleukin-8; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10