Lymphocytes utilize CD11b/CD18 for adhesion to Candida albicans.
Large granular lymphocytes require adherence to hyphae of Candida albicans to inhibit growth of this fungus. This study was undertaken to identify the lymphocyte surface structures that mediate this adhesion. Monoclonal antibodies specific for epitopes of the alpha subunit (CD11b) and the beta 2 subunit (CD18) of Mac-1 eliminated lymphocyte adhesion to C. albicans hyphae. Significant inhibition of lymphocyte adhesion to C. albicans was also achieved with known protein ligands of Mac-1. These proteins included the extracellular matrix proteins vitronectin, laminin, and fibrinogen as well as two engineered peptides containing RGD (arginine-glycine-aspartic acid) sequences. Carbohydrates including N-acetyl-D-glucosamine which have been demonstrated to inhibit Mac-l-mediated adhesion to whole yeast and yeast zymosan also blocked lymphocyte adhesion to hyphae. These results identify Mac-1 (CD11b/CD18) as the surface structure that mediates lymphocyte adhesion to C. albicans. A model is proposed for lymphocyte Mac-1 activation by microbial ligands.
Forsyth
C B
CB
Department of Microbiology and Immunology, Stritch School of Medicine, University of Chicago, Maywood, Illinois 60153, USA.
Mathews
H L
HL
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D000935:Antifungal Agents / Q000494:pharmacology; D001667:Binding, Competitive / Q000276:immunology; D015153:Blotting, Western; D018821:CD18 Antigens / Q000276:immunology* / Q000302:isolation & purification; D002176:Candida albicans / Q000254:growth & development / Q000276:immunology*; D002241:Carbohydrates / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects / Q000276:immunology*; D016326:Extracellular Matrix Proteins / Q000494:pharmacology; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology*; D016177:Macrophage-1 Antigen / Q000276:immunology* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000276:immunology / Q000494:pharmacology; D011233:Precipitin Tests
D000911:Antibodies, Monoclonal; D000935:Antifungal Agents; D018821:CD18 Antigens; D002241:Carbohydrates; D016326:Extracellular Matrix Proteins; D016177:Macrophage-1 Antigen; D009842:Oligopeptides; C047981:arginyl-glycyl-aspartic acid