Interleukin 4 (IL-4) blocks the IL-2-induced increased in natural killer activity and DNA synthesis of decidual CD16-CD56bright NK cells by inhibiting expression of the IL-2 receptor alpha, beta, and gamma.
The natural killer (NK) activity and DNA synthesis of decidual CD16-CD56bright NK cells were markedly elevated by treatment with interleukin 2 (IL-2). IL-4 did not affect NK activity or DNA synthesis of decidual CD16-CD56bright NK cells, but inhibited the IL-2-induced NK activity and DNA synthesis in a dose-dependent manner. Flow cytometry of decidual mononuclear cells cultured in IL-2 or IL-4 or both IL-2 and IL-4 demonstrated IL-4 inhibition of the expression of the IL-2 receptor alpha (IL-2R alpha), IL-2R beta, and IL-2R gamma on decidual CD16-CD56bright NK cells. This suggests that IL-4 blocks the IL-2-induced NK activity and DNA synthesis of decidual CD16-CD56bright NK cells by inhibiting the expression of IL-2R alpha, IL-2R beta, and IL-2R gamma.
Saito
S
S
Department of Obstetrics and Gynecology, Nara Medical University, Japan.
Umekage
H
H
Nishikawa
K
K
Morii
T
T
Narita
N
N
Enomoto
M
M
Sakakura
S
S
Harada
N
N
Ichijo
M
M
Morikawa
H
H
D019002:CD56 Antigen / Q000276:immunology; D003602:Cytotoxicity, Immunologic / Q000187:drug effects* / Q000235:genetics; D004261:DNA Replication / Q000187:drug effects / Q000276:immunology*; D003656:Decidua / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D007376:Interleukin-2 / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D015847:Interleukin-4 / Q000494:pharmacology*; D007694:Killer Cells, Natural / Q000145:classification / Q000187:drug effects* / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000276:immunology; D011247:Pregnancy; D017452:Receptors, IgG / Q000276:immunology; D015375:Receptors, Interleukin-2 / Q000037:antagonists & inhibitors* / Q000096:biosynthesis / Q000187:drug effects
D019002:CD56 Antigen; D007166:Immunosuppressive Agents; D007376:Interleukin-2; D017452:Receptors, IgG; D015375:Receptors, Interleukin-2; D015847:Interleukin-4