title: Expressed sequence tags from the long arm of human chromosome 21.
abstract: We isolated expressed sequence tags (ESTs) on the long arm of chromosome 21. The ESTs were mapped by PCR using a monochromosomal somatic-cell mapping panel. Of a total of 55 cDNAs, 30 mapped back uniquely to chromosome 21, 7 mapped back to other chromosomes including chromosome 21, 8 mapped back to chromosomes other than 21, and 10 could not be assigned using this methodology. The 30 chromosome 21-specific markers so isolated represent useful EST markers. A rapid PCR-based method was used to delineate the expression pattern of these 30 pairs in different tissues.
authors.affiliation: Howard Hughes Medical Institute, University of Michigan Medical Center, MSRBI, Ann Arbor 48109-0650, USA.
authors.forename: P W
authors.initials: PW
authors.lastname: Chiang
authors.forename: G
authors.initials: G
authors.lastname: Dzida
authors.forename: J
authors.initials: J
authors.lastname: Grumet
authors.forename: J F
authors.initials: JF
authors.lastname: Cheng
authors.forename: W J
authors.initials: WJ
authors.lastname: Song
authors.forename: E
authors.initials: E
authors.lastname: Crombez
authors.forename: M L
authors.initials: ML
authors.lastname: Van Keuren
authors.forename: D M
authors.initials: DM
authors.lastname: Kurnit
mesh_terms: D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D002891:Chromosomes, Human, Pair 21; D017931:DNA Primers; D018076:DNA, Complementary; D005819:Genetic Markers; D006801:Humans; D006822:Hybrid Cells; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D016324:Sequence Tagged Sites
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D005819:Genetic Markers