Growth ability and immunological properties of Erysipelothrix rhusiopathiae serotype 2.
Five field strains of Erysipelothrix rhusiopathiae belonging to serotype 2 were compared for their growth ability, immunogenicity in mice, SDS-PAGE profile of cell surface proteins and their immunoblotting patterns. Strain Tama-96 showed the most stable growth in Feist medium and tryptose phosphate broth with Tween 80 (TPB), and its immunogenicity was highest in a mouse protection test using the inactivated vaccines prepared from 20-h TPB culture. The 50% mouse protective dose of the vaccine was only 12 microliter. SDS-PAGE and immunoblotting patterns of the proteins were similar among the strains in general and indicated that 66 to 64 kDa protein antigens were dominant.
Zarkasie
K
K
Department of Veterinary Microbiology, Nippon Veterinary and Animal Science University, Tokyo, Japan.
Sawada
T
T
Yoshida
T
T
Takahashi
I
I
Takahashi
T
T
D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis; D000954:Antigens, Surface / Q000032:analysis; D001428:Bacterial Vaccines; D003470:Culture Media; D004591:Electrophoresis, Polyacrylamide Gel; D004888:Erysipelothrix / Q000145:classification / Q000254:growth & development* / Q000276:immunology; D004889:Erysipelothrix Infections / Q000276:immunology* / Q000382:microbiology / Q000517:prevention & control; D007700:Kinetics; D051379:Mice; D012703:Serotyping; D013552:Swine; D013553:Swine Diseases; D014774:Virulence
D000942:Antigens, Bacterial; D000954:Antigens, Surface; D001428:Bacterial Vaccines; D003470:Culture Media