Analysis of STR-PCR products with high-resolution denaturing PAGE.
The Pharmacia Multiphor II horizontal electrophoresis chamber is a widespread tool for analysis of PCR products in forensic casework. Up to date, however, there is no protocol for successfully running high-resolution denaturing PAGE (poly-acrylamide gel electrophoresis) on a horizontal electrophoresis chamber. We modified the electrophoresis conditions to make this possible.
Neuhuber
F
F
Institute of Legal Medicine, University of Salzburg, Austria.
Radacher
M
M
Sorgo
G
G
D004247:DNA; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D006801:Humans; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*
D004247:DNA