Carbohydrate-deficient transferrin evaluation in dry blood spots.
The aim of this study was to assess the performance of the isoelectric focusing/immunoblotting/laser densitometry (IEF/IB/LD) procedure to evaluate carbohydrate-deficient transferrin (CDT) derived from dry blood spots. Serum specimens obtained from insurance applicants were analyzed for CDT by IEF/IB/LD. Dry blood spots derived from 50 serum specimens were analyzed by IEF/IB/LD. A comparative analysis of these serum specimens and the paired dry blood spots by IEF/IB/LD shows a highly significant correlation of the CDT values (r = 0.94, p < 0.0001). Stability studies indicate that, under proper storage conditions (2-3 days at room temperature, 2 weeks at 4 degrees C, or frozen at or below -20 degrees C indefinitely), dry blood spots can be used as a source of CDT for analysis by IEF/IB/LD, thus simplifying sampling, storage, and transportation of specimens to the testing site.
Bean
P
P
Specialty Laboratories, Inc., Santa Monica, California, USA.
Sutphin
M S
MS
Necessary
P
P
Agopian
M S
MS
Liegmann
K
K
Ludvigsen
C
C
Peter
J B
JB
D000437:Alcoholism / Q000097:blood / Q000175:diagnosis*; D001801:Blood Stains; D003720:Densitometry; D006801:Humans; D015151:Immunoblotting; D007341:Insurance; D007525:Isoelectric Focusing; D014168:Transferrin / Q000031:analogs & derivatives* / Q000032:analysis
D014168:Transferrin; C056759:carbohydrate-deficient transferrin