title: Expression of heparin-binding EGF-like growth factor in rat liver injured by carbon tetrachloride or D-galactosamine.
abstract: We reported recently that heparin-binding EGF-like growth factor (HB-EGF), a member of the EGF family, is a new hepatotrophic factor for the regeneration of rat liver after partial hepatectomy. The current study examined changes in the amount of HB-EGF mRNA in liver injured by hepatotoxins. The level of HB-EGF mRNA was very low in normal rat liver, but increased markedly in the liver of rats injured by CCl4, showing two peaks, the first at 6 h and the second at 36 h. Western blot analysis showed that HB-EGF protein in the liver of CCl4-treated rats (at 6 h) was increased about 3.4-fold above normal. The level of HB-EGF mRNA also increased markedly in the liver of rats treated with D-galactosamine, showing a major peak at 18 h, and a smaller one at 6 h. These results indicate that HB-EGF may play a role in the regeneration of the liver following hepatotoxic injury.
authors.affiliation: Second Department of Internal Medicine, Osaka University Medical School, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Kiso
authors.forename: S
authors.initials: S
authors.lastname: Kawata
authors.forename: S
authors.initials: S
authors.lastname: Tamura
authors.forename: N
authors.initials: N
authors.lastname: Ito
authors.forename: H
authors.initials: H
authors.lastname: Tsushima
authors.forename: A
authors.initials: A
authors.lastname: Yamada
authors.forename: S
authors.initials: S
authors.lastname: Higashiyama
authors.forename: N
authors.initials: N
authors.lastname: Taniguchi
authors.forename: Y
authors.initials: Y
authors.lastname: Matsuzawa
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002251:Carbon Tetrachloride; D056486:Chemical and Drug Induced Liver Injury; D004815:Epidermal Growth Factor / Q000235:genetics* / Q000378:metabolism; D005688:Galactosamine; D015870:Gene Expression; D006493:Heparin / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008107:Liver Diseases / Q000378:metabolism; D008297:Male; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D012333:RNA, Messenger; D004815:Epidermal Growth Factor; D005688:Galactosamine; D006493:Heparin; D002251:Carbon Tetrachloride