title: Expression of 16 kDa proteolipid of vacuolar-type H(+)-ATPase in human pancreatic cancer.
abstract: Recent studies have shown that bafilomycin A1-sensitive vacuolar-type H(+)-ATPase (V-ATPase) plays important roles in cell growth and differentiation. However, there is no published study that has focused on the expression of V-ATPase in human tumour tissues. This study was designed to examine the mRNA and protein levels for the 16 kilodalton (kDa) proteolipid of V-ATPase in human pancreatic carcinoma tissues. We first investigated the mRNA level for V-ATPase in six cases of invasive pancreatic cancers and two normal pancreases, using reverse transcription-polymerase chain reaction technique. Then, we examined immunohistochemically the level of V-ATPase protein in 49 pancreatic cancers and ten benign cystic neoplasms of the pancreas, using antisera raised against the 16 kDa proteolipid. There was a notable difference in the level of V-ATPase mRNA between normal and pancreatic carcinoma tissues, with no evident difference in the expression of the beta-actin gene. Immunohistochemically, 42 out of 46 invasive ductal cancers (92%) displayed a mild to marked immunoreactivity for V-ATPase in the cytoplasm, whereas neither non-invasive ductal cancers nor benign cystic neoplasms expressed detectable immunoreactive proteins. These findings suggest that the overexpression of V-ATPase protein is characteristic of invasive pancreatic tumours. V-ATPase may play some crucial roles in tumour progression.
authors.affiliation: Department of Surgery (II), School of Medicine, Kanazawa University, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Ohta
authors.forename: M
authors.initials: M
authors.lastname: Numata
authors.forename: H
authors.initials: H
authors.lastname: Yagishita
authors.forename: F
authors.initials: F
authors.lastname: Futagami
authors.forename: Y
authors.initials: Y
authors.lastname: Tsukioka
authors.forename: H
authors.initials: H
authors.lastname: Kitagawa
authors.forename: M
authors.initials: M
authors.lastname: Kayahara
authors.forename: T
authors.initials: T
authors.lastname: Nagakawa
authors.forename: I
authors.initials: I
authors.lastname: Miyazaki
authors.forename: M
authors.initials: M
authors.lastname: Yamamoto
authors.forename: S
authors.initials: S
authors.lastname: Iseki
authors.forename: S
authors.initials: S
authors.lastname: Ohkuma
mesh_terms: D000230:Adenocarcinoma / Q000201:enzymology* / Q000473:pathology; D000328:Adult; D000368:Aged; D000595:Amino Acid Sequence; D001483:Base Sequence; D018270:Carcinoma, Ductal, Breast / Q000201:enzymology* / Q000473:pathology; D018450:Disease Progression; D005260:Female; D006801:Humans; D046911:Macromolecular Substances; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D010179:Pancreas / Q000201:enzymology; D010190:Pancreatic Neoplasms / Q000201:enzymology* / Q000473:pathology; D011510:Proteolipids / Q000096:biosynthesis*; D006180:Proton-Translocating ATPases / Q000096:biosynthesis* / Q000276:immunology; D012333:RNA, Messenger / Q000378:metabolism*; D012016:Reference Values; D014617:Vacuoles / Q000201:enzymology
chemical_list: D046911:Macromolecular Substances; D011510:Proteolipids; D012333:RNA, Messenger; D006180:Proton-Translocating ATPases