Cloning and sequencing of the first plant GlnRS and GluRS genes.
We have cloned and sequenced glutamate-tRNA synthetase (GluRS) and glutaminyl-tRNA synthetase (GlnRS) from Arabidopsis thaliana. They have conservative motifs found in all known GlxRS genes. For Lupinus luteus we found only one gene of GlxRS. At the moment we do not know exactly, whether it corresponds to GlnRS or GluRS.
Siatecka
M
M
Institute of Bioorganic Chemistry, Polish Academy of Science, Poznan, Poland.
Rozek
M
M
Ignacak
M
M
Barciszewski
J
J
D000595:Amino Acid Sequence; D000604:Amino Acyl-tRNA Synthetases / Q000235:genetics*; D000818:Animals; D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics; D003001:Cloning, Molecular; D017124:Conserved Sequence; D018076:DNA, Complementary / Q000235:genetics; D007887:Fabaceae / Q000201:enzymology / Q000235:genetics; D017343:Genes, Plant; D005975:Glutamate-tRNA Ligase / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D010946:Plants, Medicinal; D016133:Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D000604:Amino Acyl-tRNA Synthetases; D005975:Glutamate-tRNA Ligase; C020659:glutaminyl-tRNA synthetase