Obese gene expression alters the ability of 30A5 preadipocytes to respond to lipogenic hormones.
Leptin, the product of the ob gene, controls food-intake and weight loss in the ob mouse. Although the target(s) of the circulating leptin is presumed to be the brain which then orchestrates food-intake and weight loss, how leptin functions in the process of weight loss is unknown. In this report, we present evidence that ob gene expression in cultured cells suppresses acetyl-CoA carboxylase gene expression and lipid synthesis which are induced by hormone treatment. This is the first example in which leptin has been found to suppress defined biochemical reactions that contribute to lipid accumulation without the participation of the brain.
Bai
Y
Y
Department of Biochemistry, Purdue University, West Lafayette, Indiana 47907, USA.
Zhang
S
S
Kim
K S
KS
Lee
J K
JK
Kim
K H
KH
D000103:Acetyl-CoA Carboxylase / Q000096:biosynthesis*; D017667:Adipocytes; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003470:Culture Media; D017931:DNA Primers; D018076:DNA, Complementary; D003907:Dexamethasone / Q000494:pharmacology*; D005227:Fatty Acids / Q000096:biosynthesis; D015870:Gene Expression / Q000187:drug effects; D005993:Glycerolphosphate Dehydrogenase / Q000378:metabolism; D007328:Insulin / Q000494:pharmacology*; D007700:Kinetics; D020738:Leptin; D008055:Lipids / Q000096:biosynthesis*; D051379:Mice; D008969:Molecular Sequence Data; D009765:Obesity / Q000235:genetics; D016133:Polymerase Chain Reaction; D014176:Protein Biosynthesis; D011506:Proteins / Q000494:pharmacology; D011994:Recombinant Proteins / Q000096:biosynthesis; D013489:Suppression, Genetic; D014162:Transfection
D003470:Culture Media; D017931:DNA Primers; D018076:DNA, Complementary; D005227:Fatty Acids; D007328:Insulin; D020738:Leptin; D008055:Lipids; D011506:Proteins; D011994:Recombinant Proteins; D003907:Dexamethasone; D005993:Glycerolphosphate Dehydrogenase; D000103:Acetyl-CoA Carboxylase