Spermatocyte-specific expression of the gene for mouse testis-specific transcription elongation factor S-II.
Previously, we characterized a rat cDNA for testis-specific transcription elongation factor S-II (SII-T1) (Q. Xu et al., J. Biol. Chem. 269, 3100-3103 (1994)). Here, we isolated a 335-bp fragment of the cDNA for mouse SII-T1, and used it to examine the expression of the SII-T1 gene in the testis by in situ hybridization. The results indicated that the SII-T1 gene is expressed exclusively in spermatocytes, showing no appreciable expression in spermatogonia, spermatids, or Leydig cells. RT-PCR experiments using testis RNA from W/Wv mutant mice also suggested that SII-T1 is a specific transcription elongation factor essential for spermatogenesis.
Ito
T
T
Faculty of Pharmaceutical Sciences, University of Tokyo, Japan.
Xu
Q
Q
Takeuchi
H
H
Kubo
T
T
Natori
S
S
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis; D018076:DNA, Complementary / Q000235:genetics; D005786:Gene Expression Regulation; D017403:In Situ Hybridization; D008297:Male; D051379:Mice; D008817:Mice, Mutant Strains; D008969:Molecular Sequence Data; D009928:Organ Specificity; D012333:RNA, Messenger / Q000032:analysis; D017386:Sequence Homology, Amino Acid; D013090:Spermatocytes / Q000737:chemistry / Q000378:metabolism*; D013737:Testis / Q000737:chemistry / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics*; D035165:Transcription Factors, General; D035602:Transcriptional Elongation Factors
D018076:DNA, Complementary; D012333:RNA, Messenger; D014157:Transcription Factors; D035165:Transcription Factors, General; D035602:Transcriptional Elongation Factors; C018648:transcription factor S-II; D004247:DNA