Photo-patterning of sensor surfaces with biomolecular structures: characterisation using AFM and fluorescence microscopy.
The miniaturisation of biosensors has resulted in the need to develop techniques for the high resolution patterning of different biological molecules onto surfaces. In this paper, we describe a procedure for the selective deposition of antibodies using biological self-assembly with photo-activation of a bound ligand, and we will detail methods which may subsequently be used to characterise the resultant biomolecular constructs.
Morgan
H
H
Department of Electronics & Electrical Engineering, University of Glasgow, UK.
Pritchard
D J
DJ
Cooper
J M
JM
D000911:Antibodies, Monoclonal; D001360:Avidin / Q000737:chemistry; D001386:Azides / Q000737:chemistry; D015374:Biosensing Techniques; D001710:Biotin / Q000031:analogs & derivatives / Q000737:chemistry; D018625:Microscopy, Atomic Force / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods; D010777:Photochemistry; D012822:Silicon Dioxide
D000911:Antibodies, Monoclonal; D001386:Azides; D001360:Avidin; D001710:Biotin; D012822:Silicon Dioxide; C045549:photobiotin