Human high affinity, Na(+)-dependent L-glutamate/L-aspartate transporter GLAST-1 (EAAT-1): gene structure and localization to chromosome 5p11-p12.
The gene of the human L-glutamate transporter hGLAST-1 (EAAT-1) has been isolated and characterized. The 1626 bp cDNA open reading frame (542 aa) is distributed over ten exons and at least 85 kb on chromosome 5p11-p12. The gene is unrelated to any other previously described neurotransmitter transporter gene family, but its exon/intron structure corresponds largely to that of the Na(+)-dependent neutral amino acid transporter ASCT-1. GLAST-1, ASCT-1 and the glutamate transporters GLT-1 and EAAC-1 have strongly similar amino acid sequences. The L-glutamate transporter gene structures might help to understand the correlation of L-glutamate reuptake in neurodegenerative disorders.
Stoffel
W
W
Institut für Biochemie, Universität zu Köln, Germany. akc03@rs1.rrz.uni-kocln.de
Sasse
J
J
Düker
M
M
Müller
R
R
Hofmann
K
K
Fink
T
T
Lichter
P
P
D000595:Amino Acid Sequence; D027322:Amino Acid Transport System X-AG; D001483:Base Sequence; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics*; D002874:Chromosome Mapping; D002895:Chromosomes, Human, Pair 5; D003001:Cloning, Molecular; D004247:DNA; D006023:Glycoproteins / Q000737:chemistry / Q000235:genetics*; D006801:Humans; D007438:Introns; D008969:Molecular Sequence Data; D012964:Sodium / Q000494:pharmacology*
D027322:Amino Acid Transport System X-AG; D002352:Carrier Proteins; D006023:Glycoproteins; D004247:DNA; D012964:Sodium