title: Bacterial contamination of anaerobic vitreous cultures: using techniques employed for endophthalmitis.
abstract: OBJECTIVE
  To investigate the occurrence of contaminated cultures of vitreous specimens from non-infected eyes obtained using anaerobic techniques employed for endophthalmitis.
  
  
  METHODS
  Vitreous specimens were obtained using meticulous sterile techniques employed for endophthalmitis from seventeen patients undergoing pars plana vitrectomy for non-infective indications: vitreous hemorrhage (12 eyes), retinal detachment (3), Coat's disease (1), and congenital dislocated lens(1). Vitreous specimens were inoculated in the operating room onto an anaerobic blood agar plate and into thioglycolate broth. Bacterial growth occurring before 10 days was considered positive.
  
  
  RESULTS
  Three organisms were isolated from three separate eyes. One colony of Staphylococcus species was isolated on an anaerobic blood agar plate on day 3. A single colony of Propionibacterium acnes grew on an anaerobic blood agar plate on day 6. Alpha-hemolytic streptococci grew from thioglycolate broth on day 10.
  
  
  CONCLUSIONS
  Growth as detected in this study might represent contaminating rather than an infecting organism in an eye suspected of having endophthalmitis.
authors.affiliation: Department of Microbiology, Cleveland Clinic Foundation, 9510 Euclid Avenue, Desk A31, Cleveland, OH 44195, USA.
authors.forename: K C
authors.initials: KC
authors.lastname: Chern
authors.forename: D M
authors.initials: DM
authors.lastname: Meisler
authors.forename: G S
authors.initials: GS
authors.lastname: Hall
authors.forename: S M
authors.initials: SM
authors.lastname: Meyers
authors.forename: R E
authors.initials: RE
authors.lastname: Foster
authors.forename: Z N
authors.initials: ZN
authors.lastname: Zakov
authors.forename: C Y
authors.initials: CY
authors.lastname: Lowder
mesh_terms: D000328:Adult; D000368:Aged; D000693:Anaerobiosis; D001424:Bacterial Infections / Q000175:diagnosis / Q000382:microbiology; D001431:Bacteriological Techniques; D009877:Endophthalmitis / Q000175:diagnosis / Q000382:microbiology*; D005189:False Positive Reactions; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D011425:Propionibacterium acnes / Q000302:isolation & purification; D013210:Staphylococcus / Q000302:isolation & purification; D013291:Streptococcus / Q000302:isolation & purification; D014822:Vitreous Body / Q000382:microbiology*