Characterization of Saccharomyces cerevisiae deficient in expression of phospholipase D.
A gene encoding phospholipase D (PLD) in Saccharomyces cerevisiae was identified. The 195 kDa product of PLD1 has 24% overall sequence identity with a plant PLD. Expression of yeast PLD activity was eliminated by one-step gene disruption. Yeast haploids lacking PLD activity were deficient in growth on non-fermentable carbon sources. Diploids lacking expression of PLD1 were unable to sporulate.
Ella
K M
KM
Department of Cell and Molecular Pharmacology, Medical University of South Carolina, Charleston, 29425-2251, USA.
Dolan
J W
JW
Qi
C
C
Meier
K E
KE
D000595:Amino Acid Sequence; D001483:Base Sequence; D002855:Chromatography, Thin Layer; D003001:Cloning, Molecular; D017931:DNA Primers / Q000737:chemistry; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D010641:Phenotype; D010739:Phospholipase D / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D011003:Ploidies; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics / Q000502:physiology; D017421:Sequence Analysis; D017384:Sequence Deletion; D017386:Sequence Homology, Amino Acid; D013172:Spores, Fungal / Q000502:physiology; D014170:Transformation, Genetic
D017931:DNA Primers; D010739:Phospholipase D