Sequence analysis of the 5'-untranslated region of the human H1 histamine receptor-encoding gene.
A clone containing the H1 histamine receptor (H1HR)-encoding gene was isolated from a human genomic DNA library. The 5'-UTR of the H1HR gene reported here differs upstream from bp -142 from that reported previously [Fukui et al., Biochem. Biophys. Res. Comm. 201 (1994) 894-901]. PCR amplification utilizing primer pairs derived from the 5'-UTR reported herein amplified a DNA fragment of the expected size from human genomic DNA whereas 5'-UTR primers derived from the Fukui et al. sequence did not yield a PCR product. The 5'-UTR of H1HR contains potential TATA and CCAAT boxes, a CACCC sequence, potential GREs and other DNA-binding motifs.
Max
S I
SI
Department of Molecular and Cellular Biology, Institute for Genomic Research, Rockville, MD 20850, USA.
Chowdhury
B A
BA
Fraser
C M
CM
D001483:Base Sequence; D001665:Binding Sites; D015139:Blotting, Southern; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D014176:Protein Biosynthesis; D011965:Receptors, Glucocorticoid / Q000378:metabolism; D011969:Receptors, Histamine H1 / Q000235:genetics* / Q000378:metabolism; D017422:Sequence Analysis, DNA; D016385:TATA Box
D011965:Receptors, Glucocorticoid; D011969:Receptors, Histamine H1