title: Localization and physical mapping of genes encoding the A+U-rich element RNA-binding protein AUF1 to human chromosomes 4 and X.
abstract: Messenger RNAs encoding many oncoproteins and cytokines are relatively unstable. Their instability, which ensures appropriate levels and timing of expression, is controlled in part by proteins that bind to A+U-rich instability elements (AREs) present in the 3'-untranslated regions of the mRNAs. cDNAs encoding the AUF1 family of ARE-binding proteins were cloned from human and murine cDNA libraries. In the present study monochromosomal somatic cell hybrids were used to localize two AUF1 loci to human chromosomes 4 and X. In situ hybridization analyses using P1 clones as probes identified the 4q21.1-q21.2 and Xq12 regions as the locations of the AUF1 genes.
authors.affiliation: Department of Microbiology, Bowman Gray School of Medicine of Wake Forest University, Winston-Salem, North Carolina, 27157, USA.
authors.forename: B J
authors.initials: BJ
authors.lastname: Wagner
authors.forename: L
authors.initials: L
authors.lastname: Long
authors.forename: P N
authors.initials: PN
authors.lastname: Rao
authors.forename: M J
authors.initials: MJ
authors.lastname: Pettenati
authors.forename: G
authors.initials: G
authors.lastname: Brewer
mesh_terms: D000818:Animals; D001482:Base Composition; D001665:Binding Sites; D002874:Chromosome Mapping; D002894:Chromosomes, Human, Pair 4; D003001:Cloning, Molecular; D018076:DNA, Complementary; D015723:Gene Library; D000081035:Heterogeneous Nuclear Ribonucleoprotein D0; D034502:Heterogeneous-Nuclear Ribonucleoprotein D; D006801:Humans; D006822:Hybrid Cells; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D012333:RNA, Messenger / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000378:metabolism; D014960:X Chromosome
chemical_list: D018076:DNA, Complementary; C000630986:HNRNPD protein, human; D000081035:Heterogeneous Nuclear Ribonucleoprotein D0; D034502:Heterogeneous-Nuclear Ribonucleoprotein D; C000630987:Hnrpd protein, mouse; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D011994:Recombinant Proteins