[Low incidence of mutations in codon 12 of the c-K-ras gene in bladder cancer].
During vesical carcinogenesis a variety of genetic alterations such as oncogene mutation or loss of suppressor genes have been detected. Codon 12 mutation of the c-K-ras gene has been seen with a high frequency in several human neoplasias but its participation in the development of vesical cancers has not been fully dilucidated. Using the DNA restriction fragments polymorphism (RFLP) technique enhanced by a polymerase chain reaction (PCR) a study has been made of codon 12 mutation at the c-K-ras gene in 55 patient with vesical cancer undergoing surgery between 1991 and 1992. The tumoral stage was superficial (Ta-Tl) in 24 cases, infiltrant (T2-T4) in 28 cases and unknown in 3 cases. Two patients (3.6%) showed codon 12 mutation at the c-K-ras gene. One case was a fast evolving infiltrant tumour (T2-T3) which caused death of the patient after 4 months while the other case was a surface tumour (G2Ta) which relapsed early, the pathological anatomy revealing a stage T2-T3 squamous carcinoma. Our results suggest that codon 12 mutation at the c-K-ras gene is not a meaningful genetic change in the genesis of vesical cancer. Its emergence, however, appears to be related to a more aggressive tumoural behaviour.
Ejarque
M J
MJ
Servicio de Urología, Hospital de la Santa Cruz y San Pablo, Barcelona.
Villanueva
A
A
Chechile
G
G
Regalado
R
R
Errando
C
C
Algaba
F
F
Capella
G
G
Lluis
F
F
Villavicencio
H
H
Vicente
J
J
D000368:Aged; D000369:Aged, 80 and over; D001483:Base Sequence; D002295:Carcinoma, Transitional Cell / Q000235:genetics*; D003062:Codon; D004252:DNA Mutational Analysis; D005260:Female; D011905:Genes, ras / Q000235:genetics*; D006801:Humans; D015994:Incidence; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009154:Mutation; D001749:Urinary Bladder Neoplasms / Q000235:genetics*
D003062:Codon
Baja incidencia de mutación del codon 12 del gen c-K-ras en el cáncer vesical.