Characterization of the NHA1 gene encoding a Na+/H+-antiporter of the yeast Saccharomyces cerevisiae.
The NHA1 gene (2958 nt) encoding a putative Na(+)/H(+) antiporter (986 aa) in Saccharomyces cerevisiae was cloned by selection based on increased NaCl tolerance. The putative protein is highly similar to sodium/proton antiporters from Schizosaccharomyces pombe (gene sod2), and Zygosaccharomyces rouxii (gene Z-SOD2). Overexpression of the NHA1 gene results in higher and partially pH-dependent tolerance to sodium and lithium; its disruption leads to an increased sensitivity towards these ions.
Prior
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Laboratoire de Microbiologie et de Genetique, URA n-1481 Universite Louis Pasteur, Strasbourg, France.
Potier
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Souciet
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Sychrova
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D000595:Amino Acid Sequence; D001483:Base Sequence; D027682:Cation Transport Proteins; D003001:Cloning, Molecular; D004271:DNA, Fungal; D008565:Membrane Proteins / Q000378:metabolism*; D008969:Molecular Sequence Data; D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins; D017386:Sequence Homology, Amino Acid; D012965:Sodium Chloride / Q000494:pharmacology; D017923:Sodium-Hydrogen Exchangers / Q000235:genetics / Q000378:metabolism*
D027682:Cation Transport Proteins; D004271:DNA, Fungal; D008565:Membrane Proteins; C000631624:NHA1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D017923:Sodium-Hydrogen Exchangers; D012965:Sodium Chloride