Tentative direct antimicrobial susceptibility testing in urine.
OBJECTIVE
Tentative direct disk susceptibilities in bacteriuria compared to standard techniques were reevaluated.


METHODS
A sterile cotton swab was saturated with urine and uniformly streaked over the surface of a Mueller-Hinton agar plate. Susceptibility disks were added within 5 minutes and the zone of inhibition around the antibiotic disk was read after 16 to 18 hours of incubation.


RESULTS
The tentative direct susceptibilities showed an accuracy of 98.2% compared to standard techniques in 2,906 positive pure cultures. The false-positive rate was 0.53% and the false-negative rate was 0.14%.


CONCLUSIONS
Results of tentative direct antibiotic susceptibility in bacteriuria are comparable to those of standard techniques and are obtained 24 hours sooner.
Gillenwater
J Y
JY
Department of Urology, University of Virginia, Charlottesville, USA.
Clark
M M
MM
D001419:Bacteria / Q000187:drug effects; D005188:False Negative Reactions; D005189:False Positive Reactions; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods*; D014556:Urine / Q000382:microbiology*