Improved cloning of antibody variable regions from hybridomas by an antisense-directed RNase H digestion of the P3-X63-Ag8.653 derived pseudogene mRNA.
Ostermeier
C
C
Max-Planck-Institut für Biophysik, Frankfurt, Germany.
Michel
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H
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular; D003576:Electron Transport Complex IV / Q000276:immunology; D006825:Hybridomas / Q000276:immunology*; D007135:Immunoglobulin Variable Region / Q000235:genetics*; D008969:Molecular Sequence Data; D011544:Pseudogenes; D016372:RNA, Antisense / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D016914:Ribonuclease H / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D007135:Immunoglobulin Variable Region; D016372:RNA, Antisense; D012333:RNA, Messenger; D003576:Electron Transport Complex IV; D016914:Ribonuclease H