Expression of ferredoxin-NADP+ reductase in heterocysts from Anabaena sp.
The expression of ferredoxin-NADP+ reductase (FNR) from Anabaena sp. PCC 7119 in heterocysts and vegetative cells has been quantified. Specific reductase activity in heterocysts was approximately 10 times higher than in vegetative cells, corresponding to the increased FNR protein content. This was confirmed by immunoquantification of the FNR protein from whole filaments of Anabaena sp. PCC 7120 grown in media with and without combined nitrogen. Transcription of the petH gene was markedly enhanced in the absence of combined nitrogen. This suggests that the increased RNA level is mainly responsible for the up-regulation of FNR in heterocysts. As has been observed for nif genes, iron deficiency also increased transcription of petH. Characterization of the FNR purified from isolated heterocysts showed no detectable differences from the enzyme from vegetative cells. Although nitrogen stress was a key regulatory factor, localization of the petH gene in the genomic map of Anabaena PCC 7120 showed that this gene is not physically associated with the nif cluster.
Razquin
P
P
Departamento de Bioquímica y Biología Molecular y Cellular, Facultad de Ciencias, Universidad de Zaragoza, Spain.
Fillat
M F
MF
Schmitz
S
S
Stricker
O
O
Böhme
H
H
Gómez-Moreno
C
C
Peleato
M L
ML
D017033:Anabaena / Q000201:enzymology / Q000502:physiology*; D001426:Bacterial Proteins / Q000032:analysis / Q000378:metabolism*; D015152:Blotting, Northern; D029968:Escherichia coli Proteins; D005287:Ferredoxin-NADP Reductase / Q000096:biosynthesis* / Q000302:isolation & purification; D015870:Gene Expression; D018506:Gene Expression Regulation, Plant; D007506:Iron-Sulfur Proteins / Q000032:analysis / Q000378:metabolism*; D014157:Transcription Factors
D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; C060704:FNR protein, E coli; D007506:Iron-Sulfur Proteins; D014157:Transcription Factors; D005287:Ferredoxin-NADP Reductase