title: SNAP-25 is differentially expressed by noradrenergic and adrenergic chromaffin cells.
abstract: This study examines chromaffin cell expression of the synaptosomal-associated protein SNAP-25 in the adrenal medulla by immunoblotting, immunocytochemistry and PCR. Both mRNAs coding for the SNAP-25 isoforms a and b were detected and SNAP-25 was found to be present in all chromaffin cells in adult rat adrenal gland sections. It was essentially restricted to a zone close to the cytoplasmic face of the plasma membrane in the majority of cells, but located extensively throughout the cytoplasm in a chromaffin cell sub-population, identified by double immunofluorescence labelling to have a noradrenergic phenotype. This differential SNAP-25 expression may reflect different stages in the phenotypic development of the sympathoadrenal lineage and be related to an additional functional role in noradrenergic chromaffin cells not associated with secretion.
authors.affiliation: Unité INSERM U-338-Biologie de la Communication Cellulaire, Centre de Neurochimie, Strasbourg, France.
authors.forename: R
authors.initials: R
authors.lastname: Kannan
authors.forename: N J
authors.initials: NJ
authors.lastname: Grant
authors.forename: D
authors.initials: D
authors.lastname: Aunis
authors.forename: K
authors.initials: K
authors.lastname: Langley
mesh_terms: D000313:Adrenal Medulla / Q000166:cytology / Q000201:enzymology / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D002837:Chromaffin Granules / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism; D015870:Gene Expression / Q000235:genetics; D015151:Immunoblotting; D007150:Immunohistochemistry; D008565:Membrane Proteins / Q000378:metabolism; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000235:genetics*; D016716:PC12 Cells; D010641:Phenotype; D010625:Phenylethanolamine N-Methyltransferase / Q000032:analysis* / Q000276:immunology; D016133:Polymerase Chain Reaction; D011188:Potassium / Q000494:pharmacology; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D050825:Synaptosomal-Associated Protein 25
chemical_list: D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; C494210:Snap25 protein, rat; D050825:Synaptosomal-Associated Protein 25; D010625:Phenylethanolamine N-Methyltransferase; D011188:Potassium