title: Modulation of collagen gel contraction by decorin.
abstract: The small dermatan sulphate protein decorin interacts via its core protein with fibrillar collagens, and its glycosaminoglycan chains were proposed to be capable of self-association. It was therefore of interest to study the role of decorin in the contraction of cell-populated collagen lattices. Stable transfection of dihydrofolate reductase-deficient CHO cells with decorin cDNA resulted in impaired collagen lattice contraction. Using normal human skin fibroblasts in serum-free cultures, inclusion of 0.3 microM decorin in the culture medium also led to a delayed collagen gel contraction. Protein-free dermatan sulphate and the dermatan sulphate-degrading enzyme chondroitin ABC lyase were ineffective. Potential interactions between dermatan sulphate chains were studied by gel filtration. A shift in the elution position of [35S]sulphate-labelled decorin-derived glycosaminoglycans by unlabelled decorin could be observed only when the chains were prepared by trypsin. Chains liberated by beta-elimination or by cathepsin C were eluted at identical positions in the presence or absence of decorin. It is therefore unlikely, that the effect of decorin on collagen-gel retraction is brought about solely by glycosaminoglycan-glycosaminoglycan interactions.
authors.affiliation: Institute of Physiological Chemistry and Pathobiochemistry, University of Münster, Germany.
authors.forename: K
authors.initials: K
authors.lastname: Bittner
authors.forename: C
authors.initials: C
authors.lastname: Liszio
authors.forename: P
authors.initials: P
authors.lastname: Blumberg
authors.forename: E
authors.initials: E
authors.lastname: Schönherr
authors.forename: H
authors.initials: H
authors.lastname: Kresse
mesh_terms: D000293:Adolescent; D000818:Animals; D016466:CHO Cells; D002648:Child; D002675:Child, Preschool; D002808:Chondroitin Lyases / Q000378:metabolism; D003094:Collagen / Q000737:chemistry* / Q000187:drug effects; D006224:Cricetinae; D058575:Decorin; D003871:Dermatan Sulfate / Q000031:analogs & derivatives / Q000737:chemistry; D016326:Extracellular Matrix Proteins; D005347:Fibroblasts; D005700:Galactosyltransferases / Q000172:deficiency; D005782:Gels; D006025:Glycosaminoglycans / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D011509:Proteoglycans / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D014162:Transfection; D014357:Trypsin / Q000378:metabolism
chemical_list: C546878:DCN protein, human; D058575:Decorin; D016326:Extracellular Matrix Proteins; D005782:Gels; D006025:Glycosaminoglycans; D011509:Proteoglycans; D003871:Dermatan Sulfate; D003094:Collagen; D005700:Galactosyltransferases; C063053:xylosylprotein 4-beta-galactosyltransferase; D014357:Trypsin; D002808:Chondroitin Lyases