Toxic effects of hexane derivatives on cultured rat Schwann cells.
The cytotoxic effects of the following five hexane-related compounds were examined on Schwann cell DNA synthesis: 2,5-hexanedione (2,5-HD), 2-hexanol (2-OH), 2-hexanone (MnBK), 2,5-dimethylfuran (DF) and gamma-valerolactone (VL). Schwann cells were isolated from the sciatic nerves of neonatal Sprague-Dawley rats and cultured. [(3)H]-thymidine incorporation into Schwann cell nuclei was measured by scintillation spectrometry and autoradiography when hexane derivatives were added to the culture medium. All of the hexane-related compounds suppressed [(3)H]-thymidine incorporation in a concentration-dependent manner. DF was the most cytotoxic for the inhibition of Schwann cell DNA synthesis among the compounds. The finding suggests that DF-mediated cytotoxicity should be taken into account as a possible additional mechanism of hexane intoxication, especially in the impairment of mitotic cells.
Kamijima
M
M
Department of Hygiene, Nagoya University School of Medicine, Japan.
Sobue
G
G
Ichihara
G
G
Shibata
E
E
Ono
Y
Y
Kondo
H
H
Villanueva
M B
MB
Itoh
T
T
Mitsuma
T
T
Takeuchi
Y
Y
D000818:Animals; D001345:Autoradiography; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis / Q000187:drug effects; D006586:Hexanes / Q000633:toxicity*; D008938:Mitosis / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D012583:Schwann Cells / Q000187:drug effects*
D006586:Hexanes; D004247:DNA