Mutual interactions among ingredients of betel quid in inducing genotoxicity on Chinese hamster ovary cells.
The purpose of this study is to explore the mutual interactions among the chemical ingredients of betel quid including arecoline, sodium fluoride, catechin and glycyrrhizin in producing genotoxicity on Chinese hamster ovary (CHO) cells using the micronucleus method. Our results show that arecoline at a rather low concentration of 0.2-2 microM which could be in the oral cavity during betel quid chewing and NaF(0.8-2.4 mM) significant elevated the number of micronucleated cells in a concentration-dependent manner. In addition, significant prolongation of cell cycles was observed by treatment with arecoline (> or = 2.0 microM) or NaF (2.4 nM) in CHO cells. Both catechin and glycyrrhizin could antagonize not only the increased micronucleated cells induced by arecoline and NaF but also the prolonged cell cycle induced by arecoline in CHO cells. This find implies that the adjuvant ingredients, catechu and liquorice root extract provide not only a flavor but also an antagonist against the genotoxicity of arecoline and fluoride containing betel quid.
Lee
C H
CH
Department of Pharmacology, Chung Shan Medical and Dental College, Taichung, Taiwan.
Lin
R H
RH
Liu
S H
SH
Lin-Shiau
S Y
SY
D000818:Animals; D016587:Antimutagenic Agents / Q000494:pharmacology*; D001114:Areca; D001115:Arecoline / Q000633:toxicity*; D016466:CHO Cells; D002392:Catechin / Q000494:pharmacology*; D006224:Cricetinae; D006034:Glycyrrhetinic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D019695:Glycyrrhizic Acid; D015162:Micronucleus Tests; D009153:Mutagens / Q000633:toxicity*; D010946:Plants, Medicinal; D012969:Sodium Fluoride / Q000633:toxicity*
D016587:Antimutagenic Agents; D009153:Mutagens; D001115:Arecoline; D019695:Glycyrrhizic Acid; D002392:Catechin; D012969:Sodium Fluoride; D006034:Glycyrrhetinic Acid