title: Extraction of high quality DNA from bloodstains using diatoms.
abstract: A simple method is described for the extraction of high quality DNA for PCR amplification. The DNA was extracted by using Chelex-100 ion exchange resin or a special cell lysis buffer containing proteinase K. For further purification the DNA was bound to silica in the presence of a chaotrophic agent. Hence it is possible to unlimitedly wash the bound DNA and inhibitory substances are removed. By using diatoms as a source of silicates, this method is very economical and can therefore be used as a routine method.
authors.affiliation: Institut für Rechtsmedizin, Julius-Maximilians-Universität, Würzburg, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Günther
authors.forename: J
authors.initials: J
authors.lastname: Herold
authors.forename: D
authors.initials: D
authors.lastname: Patzelt
mesh_terms: D001801:Blood Stains; D004247:DNA / Q000302:isolation & purification*; D017377:Diatoms; D019286:Endopeptidase K; D006801:Humans; D016133:Polymerase Chain Reaction / Q000191:economics / Q000379:methods*; D012697:Serine Endopeptidases
chemical_list: D004247:DNA; D012697:Serine Endopeptidases; D019286:Endopeptidase K