Comet assay studies indicate that caffeine-mediated increase in radiation risk of embryos is due to inhibition of DNA repair.
It is well known that under specific conditions caffeine is able to enhance radiation risk of mammalian cells by a factor of approximately 1.5-2. Various mechanisms are discussed in the literature as possible explanations for this interaction. Inhibition of DNA repair plays a crucial role in the discussion, although direct evidence for this assumption is difficult to obtain. We used the "comet assay' in order to analyse the significance of repair inhibition by caffeine in the two-cell stage of mammalian gestation. Our data show that at the concentration necessary for increasing radiation risk (2 mM), caffeine effectively inhibits the restitution of radiation-damaged DNA.
Müller
W U
WU
Institut für Medizinische Strahlenbiologie, Universitätsklinikum Essen, Germany.
Bauch
T
T
Wojcik
A
A
Böcker
W
W
Streffer
C
C
D000818:Animals; D002110:Caffeine / Q000633:toxicity*; D000697:Central Nervous System Stimulants / Q000633:toxicity*; D004247:DNA / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D004249:DNA Damage; D004260:DNA Repair / Q000187:drug effects*; D004622:Embryo, Mammalian / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects*; D005260:Female; D066298:In Vitro Techniques; D051379:Mice; D009153:Mutagens / Q000633:toxicity*; D011247:Pregnancy; D012307:Risk Factors
D000697:Central Nervous System Stimulants; D009153:Mutagens; D002110:Caffeine; D004247:DNA