title: The complete sequence of a Singapore isolate of odontoglossum ringspot virus and comparison with other tobamoviruses.
abstract: The complete sequence of a Singapore isolate of odontoglossum ringspot virus (ORSV-S1) comprises 6609 nucleotides (nt) and four open reading frames (ORFs 1 to 4). The 126/183-kDa RNA-dependent RNA polymerase (RdRp), 33-kDa movement protein (MP) and 18-kDa coat protein (CP) cistrons are located at nt 63-3401/4901, 4807-5718, and 5721-6197 on the genome, respectively. The 5' UTR contains three copies of an 8-base direct repeat and (CAA)n motifs. Characteristic tRNA-like structure and three consecutive homologous regions were present in the 3' UTR. The genomic RNA and MP of ORSV-S1 are one of the longest among all members of the TOV group. Phylogenetic analysis of all four genes indicates evolutionary divergence, but within each gene there are some degrees of evolutionary convergence. The conserved amino acid sequences in the MP can be used for the classification of tobamoviruses.
authors.affiliation: Department of Botany, National University of Singapore, Singapore.
authors.forename: C G
authors.initials: CG
authors.lastname: Chng
authors.forename: S M
authors.initials: SM
authors.lastname: Wong
authors.forename: P H
authors.initials: PH
authors.lastname: Mahtani
authors.forename: C S
authors.initials: CS
authors.lastname: Loh
authors.forename: C J
authors.initials: CJ
authors.lastname: Goh
authors.forename: M C
authors.initials: MC
authors.lastname: Kao
authors.forename: M C
authors.initials: MC
authors.lastname: Chung
authors.forename: Y
authors.initials: Y
authors.lastname: Watanabe
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D016679:Genome, Viral; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D053830:Plant Viral Movement Proteins; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics; D018741:Replication Origin; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D012846:Singapore; D017898:Tobamovirus / Q000145:classification / Q000235:genetics*; D014758:Viral Core Proteins / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics
chemical_list: D053830:Plant Viral Movement Proteins; D014758:Viral Core Proteins; D014764:Viral Proteins; D012324:RNA-Dependent RNA Polymerase