title: Lysogenic conversion by a filamentous phage encoding cholera toxin.
abstract: Vibrio cholerae, the causative agent of cholera, requires two coordinately regulated factors for full virulence: cholera toxin (CT), a potent enterotoxin, and toxin-coregulated pili (TCP), surface organelles required for intestinal colonization. The structural genes for CT are shown here to be encoded by a filamentous bacteriophage (designated CTXphi), which is related to coliphage M13. The CTXphi genome chromosomally integrated or replicated as a plasmid. CTXphi used TCP as its receptor and infected V. cholerae cells within the gastrointestinal tracts of mice more efficiently than under laboratory conditions. Thus, the emergence of toxigenic V. cholerae involves horizontal gene transfer that may depend on in vivo gene expression.
authors.affiliation: Department of Microbiology and Molecular Genetics, Shipley Institute of Medicine, Harvard Medical School, Boston, Massachusetts 02115, USA.
authors.forename: M K
authors.initials: MK
authors.lastname: Waldor
authors.forename: J J
authors.initials: JJ
authors.lastname: Mekalanos
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001435:Bacteriophages / Q000235:genetics* / Q000502:physiology; D001483:Base Sequence; D002771:Cholera / Q000382:microbiology*; D002772:Cholera Toxin / Q000235:genetics*; D017931:DNA Primers; D004064:Digestive System / Q000382:microbiology; D004731:Endotoxins; D010861:Fimbriae, Bacterial / Q000502:physiology / Q000821:virology; D015870:Gene Expression; D005798:Genes, Bacterial; D008242:Lysogeny; D051379:Mice; D008969:Molecular Sequence Data; D009024:Morphogenesis; D009154:Mutation; D014161:Transduction, Genetic; D014734:Vibrio cholerae / Q000235:genetics / Q000472:pathogenicity* / Q000821:virology*; D014774:Virulence / Q000235:genetics
chemical_list: D017931:DNA Primers; D004731:Endotoxins; C077728:zonula occludens toxin, Vibrio cholerae; D002772:Cholera Toxin