Flow cytometry analysis of alpha1-adrenoceptor subtypes.
To characterize the alpha1-adrenoceptor subtypes, we developed a flow cytometry method using the fluorescent ligand BODIPY-FL prazosin and the anti-peptide antibody against the alpha1b-adrenoceptor amino terminus (designated 1B-N1-C) as probes. Three alpha1-adrenoceptors (alpha1a, alpha1b and alpha1d) expressed in CHO cells were detected by BODIPY-FL prazosin; however, only alpha1b-adrenoceptor subtype was detected by the anti-peptide antibody 1B-N1-C. Furthermore, the flow cytometry analysis with 1B-N1-C specifically identified alpha1b-adrenoceptor in native cells of hamster DDT1-MF2 cells, rat hepatocytes and cardiomyocytes.
Hirasawa
A
A
Department of Molecular, Cell Pharmacology, National Children's Medical Research Center, Tokyo, Japan.
Tsumaya
K
K
Awaji
T
T
Shibata
K
K
Homma
N
N
Shinomiya
T
T
Tsujimoto
G
G
D000595:Amino Acid Sequence; D000818:Animals; D001896:Boron Compounds / Q000737:chemistry; D016466:CHO Cells; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D006224:Cricetinae; D005434:Flow Cytometry / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D008099:Liver / Q000166:cytology; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009206:Myocardium / Q000166:cytology; D011817:Rabbits; D051381:Rats; D018340:Receptors, Adrenergic, alpha-1 / Q000032:analysis* / Q000145:classification
C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; C547713:ADRA1A protein, human; C547716:ADRA1B protein, human; C547719:ADRA1D protein, human; C547715:Adra1a protein, rat; C547718:Adra1b protein, rat; C547721:Adra1d protein, rat; D001896:Boron Compounds; D005456:Fluorescent Dyes; D018340:Receptors, Adrenergic, alpha-1