title: Identification of residues in alpha-macroglobulins important for binding to the alpha2-macroglobulin receptor/Low density lipoprotein receptor-related protein.
abstract: Variants of the receptor binding domain of both human alpha2-macroglobulin and the corresponding domain of hen egg white ovomacroglobulin have been expressed in Escherichia coli and refolded in vitro. Competition experiments with methylamine-treated alpha2-macroglobulin for binding to the multifunctional alpha2-macroglobulin receptor identify two Lys residues (residues 1370 and 1374 in human alpha2-macroglobulin) spaced by three amino acid residues as crucial for receptor binding. From this result and mutational evidence from other ligands for the alpha2-macroglobulin receptor, a tentative sequence motif for receptor binding is proposed.
authors.affiliation: Department of Chemistry, University of Aarhus, 8000 Arhus C, Denmark.
authors.forename: K L
authors.initials: KL
authors.lastname: Nielsen
authors.forename: T L
authors.initials: TL
authors.lastname: Holtet
authors.forename: M
authors.initials: M
authors.lastname: Etzerodt
authors.forename: S K
authors.initials: SK
authors.lastname: Moestrup
authors.forename: J
authors.initials: J
authors.lastname: Gliemann
authors.forename: L
authors.initials: L
authors.lastname: Sottrup-Jensen
authors.forename: H C
authors.initials: HC
authors.lastname: Thogersen
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015387:Egg Proteins, Dietary / Q000378:metabolism; D006801:Humans; D026503:Low Density Lipoprotein Receptor-Related Protein-1; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D011485:Protein Binding; D011971:Receptors, Immunologic / Q000235:genetics / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D000511:alpha-Macroglobulins / Q000235:genetics / Q000378:metabolism*
chemical_list: D015387:Egg Proteins, Dietary; D026503:Low Density Lipoprotein Receptor-Related Protein-1; D011971:Receptors, Immunologic; D000511:alpha-Macroglobulins