title: Interaction of mitochondrial elongation factors Tu.Ts with aminoacyl-tRNA.
abstract: The interaction between the bovine mitochondrial translational elongation factor Tu.Ts complex (EF-Tu.Tsmt) and aminoacyl-tRNA has been investigated using a nuclease protection assay and fluorescence enhancement of [AEDANS-s2C]Tyr-tRNA(Tyr). The equilibrium dissociation constant, Kd, for the EF-Tu.Tsmt:GTP:E. coli Phe-tRNA complex is approximately 50 nM. A similar binding constant (30 nM) is obtained using bovine mitochondrial Phe-tRNA. The equilibrium binding constant for the EF-Tu.Tsmt:GTP:yeast [AEDANS-s2C]Tyr-tRNA(Tyr) complex is approximately 4 nM when determined using the fluorescence enhancement assay.
authors.affiliation: University of North Carolina at Chapel Hill, Department of Chemistry 27599-3290, USA.
authors.forename: L A
authors.initials: LA
authors.lastname: Benkowski
authors.forename: C
authors.initials: C
authors.lastname: Takemoto
authors.forename: G
authors.initials: G
authors.lastname: Ott
authors.forename: M
authors.initials: M
authors.lastname: Beikman
authors.forename: T
authors.initials: T
authors.lastname: Ueda
authors.forename: K
authors.initials: K
authors.lastname: Watanabe
authors.forename: M
authors.initials: M
authors.lastname: Sprinzl
authors.forename: L L
authors.initials: LL
authors.lastname: Spremulli
mesh_terms: D000818:Animals; D002417:Cattle; D004926:Escherichia coli / Q000378:metabolism; D005456:Fluorescent Dyes; D066298:In Vitro Techniques; D007700:Kinetics; D008928:Mitochondria / Q000378:metabolism; D009282:Naphthalenesulfonates; D010444:Peptide Elongation Factor Tu / Q000378:metabolism*; D010445:Peptide Elongation Factors / Q000378:metabolism*; D012346:RNA, Transfer, Amino Acyl / Q000378:metabolism*; D012360:RNA, Transfer, Phe / Q000378:metabolism; D012365:RNA, Transfer, Tyr / Q000378:metabolism; D012260:Ribonucleases / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D013050:Spectrometry, Fluorescence
chemical_list: D005456:Fluorescent Dyes; D009282:Naphthalenesulfonates; D010445:Peptide Elongation Factors; D012346:RNA, Transfer, Amino Acyl; D012360:RNA, Transfer, Phe; D012365:RNA, Transfer, Tyr; C023512:elongation factor Ts; C006421:1,5-I-AEDANS; D012260:Ribonucleases; D010444:Peptide Elongation Factor Tu