title: Cloning and sequencing of the gene for Na+/H+ antiporter of Vibrio parahaemolyticus.
abstract: A gene encoding an Na+/H+ antiporter was cloned from vibrio parahaemolyticus into the plasmid pBR322 and expressed in Escherichia coli cells. The gene enabled mutant E. coli cells to grow in the presence of 0.2 M NaCl (or 10 mM LiCl). These cells were originally unable to grow under such conditions because of the lack of major Na+(Li+)/H+ antiporters. We detected Na+/H+ antiporter activity due to the gene in membrane vesicles. The gene was sequenced and the deduced amino acid sequence was found to be 72% identical to the NhaB Na+/H+ antiporter of E. coli.
authors.affiliation: Department of Microbiology, Faculty of Pharmaceutical Sciences, Okayama University, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Nozaki
authors.forename: K
authors.initials: K
authors.lastname: Inaba
authors.forename: T
authors.initials: T
authors.lastname: Kuroda
authors.forename: M
authors.initials: M
authors.lastname: Tsuda
authors.forename: T
authors.initials: T
authors.lastname: Tsuchiya
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D017923:Sodium-Hydrogen Exchangers / Q000235:genetics* / Q000378:metabolism; D014736:Vibrio parahaemolyticus / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial; D017923:Sodium-Hydrogen Exchangers