title: Diversity of the envelope glycoprotein among human immunodeficiency virus type 1 isolates of clade E from Asia and Africa.
abstract: Human immunodeficiency virus type 1 isolates of clade E, known to be largely responsible for the fulminating epidemic in Southeast Asia, have been derived exclusively from Asia and Africa. Here we provide full or partial sequences of the envelope glycoprotein gene from 13 additional clade E isolates from Asia representing patients in both early and late stages of disease. More extensive comparison of isolates within clade E by geographic locale, stage of disease, and year of isolation is now possible. The genetic diversity of clade E isolates from Asia, particularly among those derived from early-stage patients, is restricted compared with African isolates (mean interisolate distances in gp120, 5.4 and 20.2%, respectively). However, patients hospitalized with AIDS-related illnesses in Thailand harbored clade E isolates exhibiting broader interisolate diversity and with highly heterogeneous third hypervariable loop sequences. An additional pair of cysteine residues, predicting a novel disulfide bridge and present in 80% of clade E isolates from Asia, was uniformly absent from six African isolates. Clade E isolates in Thailand from early-stage subjects continue to be genetically similar to potential vaccine prototype strains, providing a favorable environment for the evaluation of genotype E candidate vaccines. However, evidence of increasing interisolate diversity is appearing among late-stage patients in Asia. This diversification of the clade E virus, if sustained, may impact preventive vaccine development strategies.
authors.affiliation: Henry M. Jackson Foundation, Rockville, Maryland 20850, USA.
authors.forename: F E
authors.initials: FE
authors.lastname: McCutchan
authors.forename: A W
authors.initials: AW
authors.lastname: Artenstein
authors.forename: E
authors.initials: E
authors.lastname: Sanders-Buell
authors.forename: M O
authors.initials: MO
authors.lastname: Salminen
authors.forename: J K
authors.initials: JK
authors.lastname: Carr
authors.forename: J R
authors.initials: JR
authors.lastname: Mascola
authors.forename: X F
authors.initials: XF
authors.lastname: Yu
authors.forename: K E
authors.initials: KE
authors.lastname: Nelson
authors.forename: C
authors.initials: C
authors.lastname: Khamboonruang
authors.forename: D
authors.initials: D
authors.lastname: Schmitt
authors.forename: M P
authors.initials: MP
authors.lastname: Kieny
authors.forename: J G
authors.initials: JG
authors.lastname: McNeil
authors.forename: D S
authors.initials: DS
authors.lastname: Burke
mesh_terms: D000349:Africa; D000595:Amino Acid Sequence; D001208:Asia; D015704:CD4 Antigens / Q000378:metabolism; D015699:HIV Envelope Protein gp120 / Q000737:chemistry; D015700:HIV Envelope Protein gp41 / Q000737:chemistry; D015497:HIV-1 / Q000737:chemistry*; D006801:Humans; D008969:Molecular Sequence Data; D014759:Viral Envelope Proteins / Q000737:chemistry*
chemical_list: D015704:CD4 Antigens; D015699:HIV Envelope Protein gp120; D015700:HIV Envelope Protein gp41; D014759:Viral Envelope Proteins