title: Isolation and characterization of mutants as an approach to a transformation system in Kluyveromyces marxianus.
abstract: A method to obtain K. marxianus mutants has been developed. Different auxotrophic mutants were isolated by nystatin and snail-enzyme enrichment procedures using an incubation time of 2 h before adding the antibiotic or the enzyme respectively. All his mutants analyzed by complementation tests turned out to belong to the same complementation group. Some of them were transformed and complemented by the S. cerevisiae HIS3 gene. These non-reverting his3 mutants contain no heterologous sequence, which is essential to make them acceptable for application in the food industry.
authors.affiliation: Bioindustry Division, Center for Genetic Engineering and Biotechnology, PO Box 6162, Havana, Cuba.
authors.forename: L
authors.initials: L
authors.lastname: Basabe
authors.forename: N
authors.initials: N
authors.lastname: Cabrera
authors.forename: V
authors.initials: V
authors.lastname: Yong
authors.forename: J
authors.initials: J
authors.lastname: Menéndez
authors.forename: J M
authors.initials: JM
authors.lastname: Delgado
authors.forename: L
authors.initials: L
authors.lastname: Rodríguez
mesh_terms: D005800:Genes, Fungal; D005816:Genetic Complementation Test; D006639:Histidine / Q000235:genetics; D007716:Kluyveromyces / Q000235:genetics*; D016296:Mutagenesis; D009154:Mutation; D010957:Plasmids / Q000235:genetics; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics; D014170:Transformation, Genetic
chemical_list: D006639:Histidine