title: Vital staining with iodine solution in delineating the border of oral dysplastic lesions.
abstract: OBJECTIVE
  The borders of dysplastic lesions of the oral mucosa are often vaguely delineated. The purpose of this study was to determine if vital staining with an iodine solution can precisely delineate the boundary of superficial spread of dysplastic or malignant epithelium.
  
  
  METHODS
  Thirty-seven biopsies were obtained from 18 patients with possible malignant lesions of the oral mucosa. Deviations between the clinical boundary obtained with vital staining and the histopathologic boundary determined in hemotoxylin-eosin-and lectin-stained sections were measured with light microscopy.
  
  
  RESULTS
  The clinical boundaries defined with the vital staining agreed well with the pathologic border lines and were in error by less than 3 mm.
  
  
  CONCLUSIONS
  This result suggests that vital staining with an iodine solution has great potential in determining the extent and precise borders of the dysplastic epithelium. A 5-mm border of normal tissue peripheral to the iodine-positive area would be adequate for the complete removal of the dysplastic epithelium.
authors.affiliation: Department of Dentistry and Oral Surgery, Shinshu University School of Medicine, Matsumoto, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Kurita
authors.forename: K
authors.initials: K
authors.lastname: Kurashina
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis*; D004848:Epithelium / Q000473:pathology; D005260:Female; D005990:Glycerol; D006801:Humans; D007455:Iodine; D017676:Lichen Planus, Oral / Q000175:diagnosis; D008297:Male; D008875:Middle Aged; D009059:Mouth Diseases / Q000175:diagnosis; D009061:Mouth Mucosa / Q000473:pathology; D009062:Mouth Neoplasms / Q000175:diagnosis*; D012996:Solutions / Q000737:chemistry; D013194:Staining and Labeling / Q000379:methods; D013431:Sulfates; D017967:Zinc Compounds; D019287:Zinc Sulfate
chemical_list: D012996:Solutions; D013431:Sulfates; D017967:Zinc Compounds; D019287:Zinc Sulfate; D007455:Iodine; D005990:Glycerol