title: Evidence for a beta 1 integrin fibronectin receptor in Candida tropicalis.
abstract: The binding of Candida tropicalis to fibronectin (FN) was studied in order to characterize the FN receptor in this species. FN binding was saturable at a concentration of 1.8 x 10(-9) M and exhibited a Kd of 2.3 x 10(-9) M and a receptor density of 854 receptors per cell. Extracts of C. tropicalis cell membrane at dilutions of 1:100-1:1000 significantly inhibited the binding of 3H-labeled FN to C. tropicalis cells (P < .03). Purified FN, antibodies to the integrin alpha 5 beta 1 (FN receptor on human placenta), and antibodies specific for the integrin beta 1 subunit recognized a C. tropicalis membrane protein of 125 +/- 25 kDa on immunoblots. Immunoprecipitation of radiolabeled proteins from C. tropicalis with purified human FN yielded a protein of 105 +/- 15 kDa. Thus, C. tropicalis expresses a protein with antigenic and functional similarity to the vertebrate beta 1 integrin FN receptor.
authors.affiliation: Department of Pediatrics, University of Minnesota, Minneapolis, USA.
authors.forename: G P
authors.initials: GP
authors.lastname: DeMuri
authors.forename: M K
authors.initials: MK
authors.lastname: Hostetter
mesh_terms: D002175:Candida / Q000145:classification / Q000378:metabolism / Q000648:ultrastructure*; D006801:Humans; D015151:Immunoblotting; D008565:Membrane Proteins / Q000378:metabolism; D011233:Precipitin Tests; D017469:Receptors, Fibronectin / Q000276:immunology / Q000302:isolation & purification*
chemical_list: D008565:Membrane Proteins; D017469:Receptors, Fibronectin