[Fluorophotometry of the animal eye].
We assessed the usefulness of the Fluorotron Master fitted with a small animal adapter. We also discuss the measurement conditions for the tupai, which is a promising experimental mammal. A good concentration for measurements with this instrument ranged from 0.5 x 10(-6) to 1.0 x 10(-6) g/ml. A suitable time for fluorophotometry of the tupai was 30 min. after injection of fluorescein-Na, and a suitable dose of the fluorescein-Na was 2 mg/kg. We could do ultrafiltration for measurement of the protein unbound fluorescein concentration from a minimal sample of the blood using a hematocrit tube, and thus could reduce the deleterious effects of blood sampling on the animal. This instrument is useful for the estimation of the blood-ocular barrier permeability of animal models.
Ishiko
S
S
Department of Ophthalmology, Asahikawa Medical College, Hokkaido, Japan.
Yoshida
A
A
Kitaya
N
N
Abiko
T
T
Mori
F
F
Kado
M
M
Takahashi
M
M
Saito
K
K
D000818:Animals; D018916:Blood-Aqueous Barrier; D001813:Blood-Retinal Barrier; D015590:Fluorophotometry; D009799:Ocular Physiological Phenomena; D014417:Tupaia