Autoantibodies to fibrillarin in systemic sclerosis (scleroderma). An immunogenetic, serologic, and clinical analysis.
OBJECTIVE
To determine the frequency, clinical associations, and any major histocompatibility complex correlations of antifibrillarin antibodies in patients with systemic sclerosis (SSc).


METHODS
Antifibrillarin antibodies were determined by indirect immunofluorescence, immunoblotting, and immunoprecipitation, and HLA class II alleles by DNA oligotyping, in a large cohort of SSc patients.


RESULTS
Antifibrillarin was found in 8% of 335 SSc sera and was significantly more common in blacks (16%) than whites (5%), in males (33%) than females (14%), and in patients with cardiac, renal, or gut involvement. The HLA class II haplotype DRB1*1302, DQB1*0604 was found significantly more frequently in SSc patients with antifibrillarin compared with race-matched normal controls and 260 SSc patients without antifibrillarin. In addition, 1 or more of the HLA-DQB1 alleles *0604, *0301, *0602, and/or *0302 was found in all antifibrillarin-positive patients, and 62% of the antifibrillarin-positive patients had 2 of these HLA-DQB1 alleles, a highly significant difference from both race-matched normal controls and antifibrillarin-negative SSc patients.


CONCLUSIONS
Antifibrillarin, although an infrequent nucleolar autoantibody, is a marker for severe SSc, especially in blacks and males, and is strongly associated with a unique HLA haplotype, as well as with combinations of certain HLA-DQB1 alleles.
Arnett
F C
FC
University of Texas-Houston Health Science Center 77030, USA.
Reveille
J D
JD
Goldstein
R
R
Pollard
K M
KM
Leaird
K
K
Smith
E A
EA
Leroy
E C
EC
Fritzler
M J
MJ
D000483:Alleles; D001323:Autoantibodies / Q000097:blood*; D001483:Base Sequence; D044383:Black People / Q000235:genetics; D002868:Chromosomal Proteins, Non-Histone / Q000276:immunology*; D015331:Cohort Studies; D005260:Female; D005802:Genes, MHC Class II / Q000235:genetics*; D006239:Haplotypes; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D009656:North America / Q000208:ethnology; D012595:Scleroderma, Systemic / Q000150:complications / Q000208:ethnology / Q000276:immunology*; D013223:Statistics as Topic; D044465:White People / Q000235:genetics
D001323:Autoantibodies; D002868:Chromosomal Proteins, Non-Histone; C047458:fibrillarin