Novel specific endonuclease activity recognizing a 10-bp sequence.
We report here the generation of a novel restriction endonuclease (ENase) activity with the 10-bp recognition sequence, [symbol: see text]. This specificity could be achieved by first methylating a substrate DNA with M.MamI in vivo, followed by in vitro R.DpnI restriction.
Striebel
H M
HM
Department of Genetics, Yale University School of Medicine, New Haven, CT 06510, USA. LEROTC@biomed.med.yale.edu
Kessler
C
C
D001482:Base Composition; D001483:Base Sequence; D004247:DNA / Q000378:metabolism; D004262:DNA Restriction Enzymes / Q000378:metabolism*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism*; D008969:Molecular Sequence Data; D013379:Substrate Specificity
D004247:DNA; D004262:DNA Restriction Enzymes; C064059:endodeoxyribonuclease BsaAI; C020102:endodeoxyribonuclease DpnI; D015252:Deoxyribonucleases, Type II Site-Specific