Female sex hormone stimulates cultured human keratinocyte proliferation and its RNA- and protein-synthetic activities.
The present study was carried out to assess the effect of female sex hormones, i.e., estrogen and progesterone, on human keratinocyte proliferation, and its RNA- and protein-synthetic activities in a culture system. The presence of receptors for estrogen and progesterone and their messenger ribonucleic acids (mRNAs) in the cultured cells was also investigated. Human keratinocytes were cultured in the experimental DMEM-Ham's F12 medium containing various concentrations of estrogen or progesterone, which was followed by determining cell yields and [3H]thymidine incorporation. The keratinocytes were also tested for RNA- and protein-synthetic activities by measuring [3H]uridine and [3H]leucine incorporation. Both estrogen and progesterone receptors were determined by the enzyme immunoassay method using monoclonal antibodies, and mRNA expression for these hormone receptors was detected by in situ hybridization. Cell yields and [3H]thymidine incorporation increased gradually until 3 x 10(-10) M of both estrogen and progesterone, decreased thereafter until 3 x 10(-7) M, and peaked at 3 x 10(-10) M. [3H]Uridine and [3H]leucine uptake followed almost the same pattern as the cell proliferation, peaking at 3 x 10(-10) M of both hormones. Small amounts of estrogen and progesterone receptors were present in the cultured cells, and their mRNAs were found to be present in the cell cytoplasm. These results clearly suggest that sex hormones play an important role in human keratinocyte proliferation, and its RNA- and protein-synthetic activities, at least in part, via their hormone receptors.
Urano
R
R
Department of Dermatology, Tokai University School of Medicine, Kanagawa, Japan.
Sakabe
K
K
Seiki
K
K
Ohkido
M
M
D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D017403:In Situ Hybridization; D015603:Keratinocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D011374:Progesterone / Q000494:pharmacology*; D014176:Protein Biosynthesis; D012313:RNA / Q000096:biosynthesis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011960:Receptors, Estrogen / Q000235:genetics / Q000378:metabolism; D011980:Receptors, Progesterone / Q000235:genetics / Q000378:metabolism
D012333:RNA, Messenger; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone; D011374:Progesterone; D004958:Estradiol; D012313:RNA