[Determination of radiochemical purity of 99mTc(Sn)-methylene diphosphonate using gel chromatography].
The paper presents results of determining radiochemical purity of the osteopathic ligand 99mTc(Sn)-methylene diphosphonate (99mTc(Sn)-MDP). Application possibilities of gel chromatography on Sephadex G 25M were investigated depending on concentrations of reagents and composition of the eluence (Ph = 4-6.5). Concentrations of the ligand were 6.3 x 10(-3) and 6.3 x 10(-1) mol/dm3 Na3 - methylene diphosphonate and of the reductant 4.2 x 10(-1) and 4.2 x 10(-5) mol/dm3 SnCl2. The ratio ligand/reductant remained constant. It was concluded that under the given experimental conditions results of analyses depend solely on the composition of the eluence. The complex partially decomposes if pure saline is used as the eluence. The best results are obtained if the eluence contained both ligand and reductant. According to the obtained results it can be recommended to use eluence with the same concentration of the reagents (excluding technetium), as in the labelled complex. Under this condition Sephadex can be applied in determining radiochemical purity of 99mTc(Sn)-methylene diphosphonate.
Vucina
J L
JL
Laboratorija za radioizotope, Institut za nuklearne nauke Vinca, Beograd.
D002850:Chromatography, Gel; D013669:Technetium Tc 99m Medronate / Q000302:isolation & purification*
C023568:technetium Tc 99m (Sn)methylenediphosphonate; D013669:Technetium Tc 99m Medronate
Odredivanje radiohemijske cistoće 99mTc(Sn)-metilen difosfonata gel hromatografijom.