Immunophenotypic analysis of hematologic malignancy by laser scanning cytometry.
The authors tested a newly-developed computerized laser scanning cytometer (LSC) as a means of performing immunophenotypic analysis of hematologic specimens within their community hospital. Results were compared on a case-by-case basis with parallel flow cytometric and immunohistochemical data. A total of 71 specimens analyzed include 22 excised lymph nodes or other tissue biopsies, 18 peripheral bloods, 17 bone marrow aspirates, 7 body fluids, and 7 fine-needle aspiration biopsies of lymphoid tissue. The LSC proved to be a useful instrument capable of generating simultaneous two-color immunofluorescent data directly analogous to that obtained via conventional flow cytometry. However, laser scanning cytometric analysis provides advantages over flow cytometric analysis, because the LSC measures cells on a slide rather than in a fluid stream. Specifically, cells can be microscopically examined at any time--before, during, or after automated immunofluorescent analysis. In addition, specimen preparation techniques are less restricted and more cost efficient. Lastly, even extremely small and/or hypocellular specimens (such as body fluids and fine-needle aspiration biopsies) can be successfully analyzed.
Clatch
R J
RJ
Department of Pathology, Christ Hospital, Oak Lawn, IL 60453, USA.
Walloch
J L
JL
Zutter
M M
MM
Kamentsky
L A
LA
D003936:Diagnosis, Computer-Assisted; D006801:Humans; D019044:Image Cytometry / Q000295:instrumentation / Q000379:methods*; D007150:Immunohistochemistry; D016130:Immunophenotyping; D007834:Lasers; D007938:Leukemia / Q000175:diagnosis*; D008223:Lymphoma / Q000175:diagnosis*