title: Preparation of pyridylaminated O-linked sugar chains from glycoproteins blotted on a polyvinylidene difluoride membrane and application to human granulocyte colony-stimulating factor.
authors.affiliation: Fuji-Gotemba Research Laboratories, Chugai Pharmaceutical Co., Ltd, Shizuoka, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Oh-eda
authors.forename: E
authors.initials: E
authors.lastname: Tominaga
authors.forename: Y
authors.initials: Y
authors.lastname: Nabuchi
authors.forename: T
authors.initials: T
authors.lastname: Matsuura
authors.forename: N
authors.initials: N
authors.lastname: Ochi
authors.forename: M
authors.initials: M
authors.lastname: Tamura
authors.forename: S
authors.initials: S
authors.lastname: Hase
mesh_terms: D000631:Aminopyridines / Q000737:chemistry; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000737:chemistry*; D006023:Glycoproteins / Q000737:chemistry*; D016179:Granulocyte Colony-Stimulating Factor / Q000737:chemistry*; D006801:Humans; D015151:Immunoblotting; D008567:Membranes, Artificial; D008969:Molecular Sequence Data; D010100:Oxygen / Q000737:chemistry; D011145:Polyvinyls; D011994:Recombinant Proteins / Q000737:chemistry; D005465:Fluorocarbon Polymers
chemical_list: D000631:Aminopyridines; D002241:Carbohydrates; D006023:Glycoproteins; D016179:Granulocyte Colony-Stimulating Factor; D008567:Membranes, Artificial; D010100:Oxygen; D011145:Polyvinyls; D011994:Recombinant Proteins; C024865:polyvinylidene fluoride; D005465:Fluorocarbon Polymers