title: Hexokinase production from S. cerevisiae. Culture conditions.
abstract: The effects of pH (4.0, 4.5, or 5.0), temperature (T) (30, 35, or 40 degrees C) and dissolved oxygen (DO) (0.2, 2.0, 4.0,or 6.0 mg O2/L) on hexokinase and invertase formation by yeast were studied. The highest enzyme activities were attained at pH 4.0, DO = 4.0 mg O2/L, and T = 35 or 40 degrees C.
authors.affiliation: Faculdade de Ciências Farmacêuticas, University of São Paulo, Brasil.
authors.forename: J
authors.initials: J
authors.lastname: Abrahão-Neto
authors.forename: P
authors.initials: P
authors.lastname: Infanti
authors.forename: M
authors.initials: M
authors.lastname: Vitolo
mesh_terms: D006026:Glycoside Hydrolases / Q000096:biosynthesis; D006593:Hexokinase / Q000096:biosynthesis*; D006863:Hydrogen-Ion Concentration; D010100:Oxygen / Q000494:pharmacology*; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000201:enzymology*; D013696:Temperature; D043324:beta-Fructofuranosidase
chemical_list: D006593:Hexokinase; D006026:Glycoside Hydrolases; D043324:beta-Fructofuranosidase; D010100:Oxygen