Viscoelasticity in wild-type and vinculin-deficient (5.51) mouse F9 embryonic carcinoma cells examined by atomic force microscopy and rheology.
We have been studying mouse F9 embryonic carcinoma cells which contain no detectable vinculin protein (5.51 cells), and compared them with F9 wild-type cells. Employing atomic force microscopy, we probed the elastic properties of individual F9 wild-type and 5.51 cells by measuring the dynamic response of controlled loads of the cantilever tip. An elastic modulus (Young) of approximately 3.8 and approximately 2.5 kPa was calculated for wild-type and 5.51 cells, respectively. Using disc rheometry, we detected a marked change in shear of a 1000g pellet of approximately 55 x 10(6) cells between wild-type and 5.51 mutants. These differences are attributed to the loss of vinculin and altered cytoskeletal organization in these cells.
Goldmann
W H
WH
Surgery Research Unit, Harvard Medical School, Charlestown, Massachusetts, 02129, USA.
Ezzell
R M
RM
D000818:Animals; D003599:Cytoskeleton / Q000502:physiology; D004548:Elasticity; D051379:Mice; D018625:Microscopy, Atomic Force; D014411:Neoplastic Stem Cells / Q000737:chemistry / Q000166:cytology*; D012212:Rheology; D016596:Vinculin / Q000172:deficiency*; D014783:Viscosity
D016596:Vinculin