title: [Diagnosis of venous thrombosis and/or pulmonary embolism by determination of d-dimers using ELISA. Review based on a study of 80 consecutive patients hospitalized in an emergency unit].
abstract: The sensitivity and specificity of an ELISA method (Fibrinostika Fbdp Organon Teknika) for assay of D-dimers in the diagnosis of deep vein thrombosis and/or pulmonary embolism was studied in 80 consecutive patients seen at an emergency unit. Fifty-six of the patients presented clinical signs of deep vein thrombosis. Diagnosis was confirmed in 26 of the 56 patients with a D-dimer level above 370 ng/ml (sensitivity 92.3%) and 370 ng/ml for 13 of 30 patients with a negative venous ultrasound Doppler examination (specificity 43.3%). The positive predictive value was 58.5% and the negative predictive value was 87%. There was a significant difference in the level of D-dimers between distal and proximal deep vein thrombosis. In 40 cases with suspected pulmonary embolis, either alone or with suspected deep vein thrombosis, diagnosis was made in only 4 of 9 with a highly or intermediately probable ventilation/perfusion scan. D-dimer level was always above 3,000 ng/ml. Coupling the ELISA dimer test with noninvasive explorations improves negative predictive value but can also avoid invasive explorations (venography, pulmonary angiography) in certain patients. A D-dimer test as sensitive as the ELISA test and as rapid as the latex test remains to be described.
authors.affiliation: Service d'Urgence Médicale et de Médecine Interne, Hôpital Edouard Herriot, Lyon.
authors.forename: C
authors.initials: C
authors.lastname: Gavaud
authors.forename: J
authors.initials: J
authors.lastname: Ninet
authors.forename: D
authors.initials: D
authors.lastname: Ville
authors.forename: B
authors.initials: B
authors.lastname: Coppere
authors.forename: M
authors.initials: M
authors.lastname: Hanss
authors.forename: P
authors.initials: P
authors.lastname: Bureau Du Colombier
authors.forename: M
authors.initials: M
authors.lastname: Vaudaine
authors.forename: M C
authors.initials: MC
authors.lastname: Trzeciak
authors.forename: J J
authors.initials: JJ
authors.lastname: Darjinoff
authors.forename: M H
authors.initials: MH
authors.lastname: Girard Madoux
authors.forename: M
authors.initials: M
authors.lastname: Dechavanne
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005338:Fibrin Fibrinogen Degradation Products / Q000032:analysis*; D006801:Humans; D008297:Male; D008875:Middle Aged; D011237:Predictive Value of Tests; D011446:Prospective Studies; D011655:Pulmonary Embolism / Q000175:diagnosis*; D012680:Sensitivity and Specificity; D013924:Thrombophlebitis / Q000175:diagnosis*
chemical_list: D005338:Fibrin Fibrinogen Degradation Products; C036309:fibrin fragment D
vernacular_title: Le diagnostic de la thrombose veineuse et/ou de l'embolie pulmonaire par le dosage des D-Dimères ELISA. Mise au point à partir d'une étude portant sur 80 patients consécutifs hospitalisés dans un service d'urgence médicale.