title: New anthracycline metabolites produced by the aklavinone 11-hydroxylase gene in Streptomyces galilaeus ATCC 31133.
abstract: Transformation of Streptomyces galilaeus ATCC 31133 with the aklavinone 11-hydroxylase gene (dnrF) resulted in the production of many red pigments. The new metabolites were purified and their structures were determined as 11-hydroxylated aclacinomycin A, B and T by spectral analysis. This result indicated that the dnrF was stably expressed in the strain S. galilaeus ATCC 31133 to give rise to hybrid aclacinomycins. In addition, a new aclacinomycin analog named 11-hydroxyaclacinomycin X was isolated from the same culture. Its structure was elucidated as 2"'-amino-11-hydroxyaclacinomycin Y. It showed strong cytotoxicity against several human tumor cell lines, especially leukemia and melanoma cell lines.
authors.affiliation: Korea Research Institute of Bioscience and Biotechnology, KIST, Taejon, Korea.
authors.forename: H S
authors.initials: HS
authors.lastname: Kim
authors.forename: Y S
authors.initials: YS
authors.lastname: Hong
authors.forename: Y H
authors.initials: YH
authors.lastname: Kim
authors.forename: O J
authors.initials: OJ
authors.lastname: Yoo
authors.forename: J J
authors.initials: JJ
authors.lastname: Lee
mesh_terms: D015250:Aclarubicin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D018943:Anthracyclines / Q000378:metabolism*; D000970:Antineoplastic Agents / Q000378:metabolism / Q000494:pharmacology*; D001189:Aryl Hydrocarbon Hydroxylases / Q000235:genetics* / Q000378:metabolism*; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D015394:Molecular Structure; D013302:Streptomyces / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism
chemical_list: C099664:11-hydroxyaclacinomycin X; D018943:Anthracyclines; D000970:Antineoplastic Agents; C011157:aclacinomycins; D015250:Aclarubicin; C090407:aklavinone 11-hydroxylase; D001189:Aryl Hydrocarbon Hydroxylases