Natural killer cell activation after interferon administration in patients with metastatic renal cell carcinoma: an ultrastructural and immunohistochemical study.
OBJECTIVE
We studied the intralesional number of natural killer (NK) cells and the ultrastructural evidence of their activation in patients with metastatic renal cell carcinoma, after preoperative interferon (IFN) administration.


METHODS
Tumor sections from 10 patients with metastatic renal cell carcinoma who received preoperative IFN (5 MU x 3 days) and from 10 patients with renal cell carcinoma were obtained. The number of NK cells was estimated immunohistochemically in paraffin sections with the CD57 monoclonal antibody and their activation was evaluated ultrastructurally by routine electron microscopy processing.


RESULTS
The number of NK cells was increased in the tumors of the IFN-administered patients (5.3 cells vs. 1.6 in the controls, p = 0.03) while their ultrastructural features revealed activation and enhanced cytolytic activity, through facilitation of granule content release.


CONCLUSIONS
IFN promotes the aggregation and activation of NK cells within the renal cell tumor, further strengthening the concept of its immunomodulatory activity.
Toliou
T
T
Theagenio Cancer Hospital, Thessaloniki, Greece.
Stravoravdi
P
P
Polyzonis
M
M
Vakalikos
J
J
D000368:Aged; D000936:Antigen-Antibody Complex; D018951:Antigens, CD20; D018959:CD57 Antigens / Q000378:metabolism; D002292:Carcinoma, Renal Cell / Q000188:drug therapy / Q000473:pathology* / Q000556:secondary / Q000648:ultrastructure; D004396:Coloring Agents; D006801:Humans; D007150:Immunohistochemistry; D007372:Interferons / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D007680:Kidney Neoplasms / Q000188:drug therapy / Q000473:pathology* / Q000648:ultrastructure; D007694:Killer Cells, Natural / Q000166:cytology / Q000187:drug effects* / Q000648:ultrastructure; D008854:Microscopy, Electron; D008875:Middle Aged; D009942:Organometallic Compounds / Q000737:chemistry
D000936:Antigen-Antibody Complex; D018951:Antigens, CD20; D018959:CD57 Antigens; D004396:Coloring Agents; D009942:Organometallic Compounds; C005460:uranyl acetate; D007372:Interferons