title: Sperm interaction with human oviductal cells in vitro.
abstract: In this article we describe the in-vitro interaction between human spermatozoa and oviductal epithelial cell monolayers. Freshly obtained spermatozoa were added to culture dishes containing human oviductal cells (co-culture), culture medium (control) or culture medium which had previously been used for culture of oviductal cells (conditioned medium). At 0, 5, 24, and 48 h of incubation the percentage of motile spermatozoa was determined and their motion characteristics analysed. Aliquots were taken to determine the percentage of acrosome-reacted spermatozoa. The spermatozoa were motile for a longer period in the presence of oviductal cells (54 +/- 9% co-culture versus 18 +/- 3% control, at 48 h) and the kinetics of the acrosome reaction exhibited a different pattern. In the control the percentage of reacted spermatozoa increased progressively throughout incubation. In co-culture, there was an increase only at 5 h; thereafter, the percentage of acrosome reactions did not change. Spermatozoa incubated in conditioned medium exhibited a behaviour halfway between the control and the co-culture. The pattern of sperm movement was not different in any of the experimental conditions. Although there was no binding between spermatozoa and oviductal epithelial cells, the frequency of the ciliary beat increased after spermatozoa were added to the oviductal cell monolayers. These results suggest that incubation with oviductal cells increases sperm survival, stabilizes the acrosome, and modifies the frequency of ciliary beat.
authors.affiliation: Unit of Reproduction and Development, Faculty of Biological Sciences, Pontificia Universidad Catolica de Chile, PO Box 114-D, Santiago, Chile.
authors.forename: P
authors.initials: P
authors.lastname: Morales
authors.forename: V
authors.initials: V
authors.lastname: Palma
authors.forename: A M
authors.initials: AM
authors.lastname: Salgado
authors.forename: M
authors.initials: M
authors.lastname: Villalon
mesh_terms: D000177:Acrosome / Q000502:physiology; D000328:Adult; D000818:Animals; D002478:Cells, Cultured; D002923:Cilia / Q000502:physiology; D017077:Culture Media, Conditioned; D004847:Epithelial Cells; D005187:Fallopian Tubes / Q000166:cytology*; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D008855:Microscopy, Electron, Scanning; D008875:Middle Aged; D013081:Sperm Motility; D013094:Spermatozoa / Q000502:physiology*
chemical_list: D017077:Culture Media, Conditioned