Temporal regulation of gene expression in adipocyte differentiation.
Cells usually become 'committed' to differentiate long before any actual morphological change is apparent. In one model commitment is a decision which corresponds to the expression of a control gene, while differentiation is the ultimate consequence of that decision. We have been studying adipocyte commitment and differentiation at the molecular level. Earlier we showed that the introduction of a specific DNA sequence into 'uncommitted' cells renders those cells committed to differentiate into adipocytes. Here we report temporal regulation of the expression of this DNA sequence; furthermore, we show that this RNA is in the non-polyA+ fraction of total cellular RNA. These data suggest that coordinate regulation of this and other genes is important in promoting differentiation.
Martino
J J
JJ
Department of Chemical Biology, Laboratory for Cancer Research, College of Pharmacy, Rutgers University, Piscataway, NJ 08855, USA.
Chen
S
S
D016475:3T3 Cells; D017667:Adipocytes / Q000166:cytology*; D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D004247:DNA / Q000235:genetics; D005786:Gene Expression Regulation / Q000502:physiology*; D006801:Humans; D051379:Mice; D012313:RNA / Q000032:analysis; D014162:Transfection
D012313:RNA; D004247:DNA