title: Evidence of involvement of CD44 in endothelial cell proliferation, migration and angiogenesis in vitro.
abstract: Angiogenesis is essential for tumor growth and metastasis. In the process of angiogenesis, the interaction between adhesive proteins of endothelial cells and extracellular matrix components plays an important role by mediating cell attachment, which is indispensable for their motility, and by transmitting the regulatory signals for cell locomotion and proliferation. In this study, we examined the hypothesis that CD44 expressed on the endothelial cell surface is involved in the angiogenesis process. The experiments using calf pulmonary artery endothelial cells (CPAE) and a human microvascular endothelial cell line (HMEC-1) show that a monoclonal antibody against CD44 (clone J 173) inhibits endothelial cell proliferation by about 30% and migration by 25-50%, and abolishes the stimulating effect of hyaluronan polysaccharides on endothelial cell migration and proliferation. This antibody also suppresses the capillary formation of CPAE in an in vitro model of angiogenesis using fibrin matrix. These results provide evidence of the involvement of endothelial-cell-associated CD44 in angiogenesis.
authors.affiliation: Inserm U 353, Institut d'Hématologie, Hôpital Saint Louis, Paris, France.
authors.forename: V
authors.initials: V
authors.lastname: Trochon
authors.forename: C
authors.initials: C
authors.lastname: Mabilat
authors.forename: P
authors.initials: P
authors.lastname: Bertrand
authors.forename: Y
authors.initials: Y
authors.lastname: Legrand
authors.forename: F
authors.initials: F
authors.lastname: Smadja-Joffe
authors.forename: C
authors.initials: C
authors.lastname: Soria
authors.forename: B
authors.initials: B
authors.lastname: Delpech
authors.forename: H
authors.initials: H
authors.lastname: Lu
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D002196:Capillaries / Q000166:cytology / Q000502:physiology; D002417:Cattle; D002455:Cell Division / Q000187:drug effects / Q000502:physiology; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology / Q000502:physiology*; D006801:Humans; D018960:Hyaluronan Receptors / Q000276:immunology / Q000502:physiology*; D006820:Hyaluronic Acid / Q000494:pharmacology; D051379:Mice; D018919:Neovascularization, Physiologic / Q000502:physiology*; D009844:Oligosaccharides / Q000494:pharmacology; D013268:Stimulation, Chemical
chemical_list: D000911:Antibodies, Monoclonal; D018960:Hyaluronan Receptors; D009844:Oligosaccharides; D006820:Hyaluronic Acid