Efficient gene transfer into mammalian cells with cholesteryl-spermidine.
The naturally occurring polyamine spermidine was covalently conjugated with cholesterol, resulting in a novel cationic compound that mediates efficient gene transfer into mammalian cells. Using reporter plasmids coding for firefly luciferase and beta-galactosidase, a simple procedure was developed allowing highly reproducible and efficient transient and stable transfection of HuH-7 cells. Transfection efficiency could be further increased when a fusogenic peptide derived from the influenza virus hemagglutinin HA2 aminoterminal sequence was included in the cholesteryl-spermidine-DNA complex. Cholesteryl-spermidine (Transfectall) represents a novel cationic compound for efficient transfection of cultured cells in vitro and has the potential to be used for gene transfer in vivo.
Moradpour
D
D
Molecular Hepatology Laboratory, Massachusetts General Hospital Cancer Center, Harvard Medical School, Charlestown, USA.
Schauer
J I
JI
Zurawski
V R
VR
Wands
J R
JR
Boutin
R H
RH
D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D002461:Cell Line, Transformed; D002784:Cholesterol / Q000737:chemistry / Q000378:metabolism*; D018014:Gene Transfer Techniques; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D006389:Hemagglutinins, Viral / Q000378:metabolism; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D013095:Spermidine / Q000737:chemistry / Q000378:metabolism*; D014162:Transfection; D014407:Tumor Cells, Cultured
D019267:Hemagglutinin Glycoproteins, Influenza Virus; D006389:Hemagglutinins, Viral; D002784:Cholesterol; D013095:Spermidine