A promiscuous T cell hybridoma restricted to various I-A molecules.
In a previous study, we identified T cell receptor and major histocompatibility complex (MHC) contact sites on the pigeon cytochrome c p43-58 peptide. Positions 46 and 54 of p43-58 were shown to be the MHC-binding sites. Specific amino acids were identified on the MHC-binding sites which bound to the relevant I-A molecule. In the present study, using NOD (I-Ag7) mice, we established a T cell hybridoma specific for a p43-58 analog 46R50E54A with arginine (R) and alanine (A) at positions 46 and 54, respectively. Interestingly, NOE 33-1-2 recognized 46R50E54A in the presence of not only I-Ag7, but also I-Ad, s, u and v. In contrast to previous reports that promiscuous T cells were able to recognize peptide antigens with various HLA-DR or I-E molecules consist of monomorphic alpha and polymorphic beta chains, the promiscuous T cell clone NOE33-1-2 recognized peptides with various I-A molecules lacking the monomorphic chain.
Katoh
M
M
Section of Pathology, Hokkaido University, Sapporo, Japan.
Itoh
Y
Y
Ogasawara
K
K
Kajino
K
K
Nishihori
H
H
Takahashi
A
A
Matsuki
N
N
Iwabuchi
K
K
Yoshida
T O
TO
Good
R A
RA
Onoé
K
K
D000595:Amino Acid Sequence; D000818:Animals; D017951:Antigen Presentation; D000949:Histocompatibility Antigens Class II / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D006825:Hybridomas / Q000737:chemistry / Q000145:classification / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D016688:Mice, Inbred NOD; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000276:immunology; D011485:Protein Binding / Q000276:immunology; D011487:Protein Conformation; D013601:T-Lymphocytes / Q000737:chemistry / Q000145:classification / Q000276:immunology*
D000949:Histocompatibility Antigens Class II; D010446:Peptide Fragments