Structural changes in fluorosed dental enamel of red deer (Cervus elaphus L.) from a region with severe environmental pollution by fluorides.
A macroscopic, microradiographic and scanning electron microscope study was performed on the structure of fluorosed dental enamel in red deer from a fluoride polluted region (North Bohemia, Czech Republic). As was revealed by analysis of mandibular bone fluoride content, the rate of skeletal fluoride accumulation in the fluorotic deer was about 6 times that in controls taken from a region not exposed to excessive fluoride deposition. In all fluorosed mandibles, the 1st molar was consistently less fluorotic than the other permanent teeth. This was related to the fact that crown formation in the M1 takes place prenatally and during the lactation period. Fluorosed teeth exhibited opaque and posteruptively stained enamel, reduction or loss of enamel ridges, moderately to grossly increased wear and, in more severe cases, also enamel surface lesions of partly posteruptive, partly developmental origin. Microradiographically, fluorosed enamel was characterised by subsurface hypomineralisation, interpreted as a result of fluoride interference with the process of enamel maturation. In addition, an accentuation of the incremental pattern due to the occurrence of alternating bands with highly varying mineral content was observed in severely fluorosed teeth, denoting fluoride disturbance during the secretory stage of amelogenesis. A corresponding enhancement of the incremental pattern was also seen in the dentine. The enamel along the more pronounced hypoplasias consisted of stacked, thin layers of crystals arranged in parallel, indicating that the ameloblasts in these locations had lost the distal (prism-forming) portions of their Tomes processes. The findings of the present study indicate that red deer are highly sensitive bioindicators of environmental pollution by fluorides.
Kierdorf
U
U
Royal Dental College, Faculty of Health Sciences, University of Aarhus, Denmark.
Kierdorf
H
H
Sedlacek
F
F
Fejerskov
O
O
D000818:Animals; D018153:Czech Republic; D003670:Deer; D003743:Dental Enamel / Q000737:chemistry / Q000473:pathology / Q000648:ultrastructure; D003744:Dental Enamel Hypoplasia / Q000209:etiology* / Q000473:pathology / Q000662:veterinary*; D004787:Environmental Pollution / Q000009:adverse effects*; D005458:Fluoride Poisoning / Q000150:complications / Q000473:pathology / Q000662:veterinary*; D008297:Male; D008852:Microradiography; D008855:Microscopy, Electron, Scanning; D008963:Molar