A nucleoside-nucleotide mixture and its components increase lymphoproliferative and delayed hypersensitivity responses in mice.
We studied the effect of individual components of a nucleoside-nucleotide mixture on T cell-mediated immunity in BALB/c and DBA/2 mice. Mice were fed for 4 wk a nucleotide-free 20% casein diet (control) or this diet supplemented with 3 mol/kg of one of the following; inosine, guanosine monophosphate, uridine, cytidine, thymidine, or a mixture of these. In both strains of mice, popliteal lymph node immunoproliferative response to alloantigeneic (C57BL/6 splenic cells) challenge in mice fed the mixture and guanosine monophosphate was greater (P < 0.05) than in the mice fed the control diet. BALB/c mice fed inosine, uridine, and thymidine also showed greater (P < 0.05) responses compared with control fed mice. In the sheep red blood cells challenge assay, foot pad weight-gain in both strains of mice fed the mixture and the individual components supplemented diets was greater (P < 0.05) than in those fed the control diet. In both strains of mice, interleukin-2 secretion by popliteal lymph node lymphocytes in mice fed the mixture and thymidine was higher (P < 0.05) compared with the rest of the groups except BALB/c mice fed cytidine. Significantly higher than control secretions of interferon-gamma were observed only in BALB/c mice fed inosine, thymidine and the mixture. We conclude that mice fed the nucleoside-nucleotide mixture and the individual components of the mixture had greater responses in the T cell-mediated immune functions studied and that the responses in mice fed the mixture were not different from those in mice fed the individual components.
Yamauchi
K
K
Department of Nutrition, Faculty of Medicine, University of the Ryukyus, Okinawa, Japan.
Adjei
A A
AA
Ameho
C K
CK
Chan
Y C
YC
Kulkarni
A D
AD
Sato
S
S
Okamoto
K
K
Yamamoto
S
S
D000818:Animals; D006157:Guanosine Monophosphate / Q000494:pharmacology; D006968:Hypersensitivity, Delayed / Q000276:immunology*; D007288:Inosine / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008811:Mice, Inbred DBA; D009705:Nucleosides / Q000494:pharmacology*; D009711:Nucleotides / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology*; D013936:Thymidine / Q000494:pharmacology; D014529:Uridine / Q000494:pharmacology
D009705:Nucleosides; D009711:Nucleotides; D007288:Inosine; D006157:Guanosine Monophosphate; D013936:Thymidine; D014529:Uridine