title: Peculiarities of the regulation of fermentation and respiration in the crabtree-negative, xylose-fermenting yeast Pichia stipitis.
abstract: The respiration of Pichia stipitis was not repressed by either high concentrations of fermentable sugars or oxygen limitation. Fermentation was not induced by high sugar concentrations, but was inactivated by aerobic conditions. The activity of pyruvate dehydrogenase was constitutive. In contrast, pyruvate decarboxylase, alcohol dehydrogenase, and aldehyde dehydrogenase were induced by a reduction in the oxygen tension. It was demonstrated that in P. stipitis, the pyruvate decarboxylase is not induced by a signal from glycolysis. Contrary to Saccharomyces cerevisiae, the pyruvate decarboxylase was not inhibited by phosphate.
authors.affiliation: Institut für Biologie IV (Mikrobiologie), RWTH Aachen, Germany.
authors.forename: V
authors.initials: V
authors.lastname: Passoth
authors.forename: M
authors.initials: M
authors.lastname: Zimmermann
authors.forename: U
authors.initials: U
authors.lastname: Klinner
mesh_terms: D000426:Alcohol Dehydrogenase / Q000096:biosynthesis / Q000378:metabolism; D000444:Aldehyde Dehydrogenase / Q000096:biosynthesis / Q000378:metabolism; D004790:Enzyme Induction; D005285:Fermentation / Q000502:physiology*; D006019:Glycolysis / Q000502:physiology; D007700:Kinetics; D010101:Oxygen Consumption / Q000502:physiology*; D010843:Pichia / Q000201:enzymology / Q000378:metabolism*; D011767:Pyruvate Decarboxylase / Q000096:biosynthesis / Q000378:metabolism; D011768:Pyruvate Dehydrogenase Complex / Q000096:biosynthesis / Q000378:metabolism; D014994:Xylose / Q000378:metabolism*
chemical_list: D011768:Pyruvate Dehydrogenase Complex; D014994:Xylose; D000426:Alcohol Dehydrogenase; D000444:Aldehyde Dehydrogenase; D011767:Pyruvate Decarboxylase