High arrythmogenesis during early reperfusion of ischaemic myocardium: participation of oxygen free radicals.
The early period of reperfusion of ischaemic myocardium leads to a high incidence of severe tachyarrhythmias including ventricular fibrillation (VF), accompanied by a sudden transitional dysfunction. Oxygen free radicals (OFR) have been identified as one of the principal factors responsible for reperfusion-induced events. However, direct evidence for participation of OFR in the arrhythmogenic mechanisms upon reperfusion is still lacking. In the present study, in isolated Langendorff-perfused rat hearts subjected to 30 min global ischaemia, the onset of reperfusion induced 100% incidence of both ventricular tachycardia (VT) and VF with their gradual cessation during 5 min of reperfusion. Generation of H2O2 in the myocardium in the first minutes of reperfusion was demonstrated by means of cerium cytochemistry. There was an increased density of cerium perhydroxide precipitate distributed throughout the myocytes and endothelial cells, confirmed by X-ray microanalysis. The mechanism of the arrhythmogenic effect of OFR may involve the inhibition of the sarcolemmal Na+/K+ ATPase activity, as was revealed by subjecting the isolated sarcolemmal fraction of rat heart to the action of an oxy-radical generating system (H2O2 + FeSO4).
Ravingerova
T
T
Institute for Heart Research, Slovak Academy of Sciences, Bratislava, Slovakia.
Slezak
J
J
Tribulova
N
N
Dzurba
A
A
Uhrik
B
B
Ziegelhoffer
A
A
D000818:Animals; D001145:Arrhythmias, Cardiac / Q000503:physiopathology*; D005609:Free Radicals; D007511:Ischemia / Q000503:physiopathology*; D008297:Male; D009206:Myocardium / Q000648:ultrastructure*; D010100:Oxygen / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D015424:Reperfusion; D013997:Time Factors
D005609:Free Radicals; D010100:Oxygen