title: Selective reduction in alpha-hydroxypalmitic acid-containing sphingomyelin and concurrent increase in hydroxylated ceramides in murine skin tumors induced by an initiation-promotion regimen.
abstract: The sphingomyelin cycle is activated to accumulate ceramides in the process of epidermal differentiation. We found that sphingomyelin in the epidermis of 4 different murine strains gave three bands on TLC, the lower band containing alpha-hydroxypalmitic acid (C16h:0(alpha)). However, in the papillomas induced in the skin of SENCAR and SSIN mice by initiation with 7,12-dimethylbenz[a]anthracene followed by promotion with 12-O-tetradecanoylphorbol acetate, the concentration of C16h:0(alpha)-containing sphingomyelin was selectively diminished with a concomitant increase in the concentrations of the ceramides containing alpha-hydroxy fatty acids. These findings indicate a possible involvement of the selective hydrolysis of alpha-hydroxy fatty acid-containing sphingomyelin in the process of tumorigenesis in mouse skin.
authors.affiliation: Department of Plastic Surgery, The University of Tokyo, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Kitano
authors.forename: Y
authors.initials: Y
authors.lastname: Iwamori
authors.forename: K
authors.initials: K
authors.lastname: Kiguchi
authors.forename: J
authors.initials: J
authors.lastname: DiGiovanni
authors.forename: T
authors.initials: T
authors.lastname: Takahashi
authors.forename: K
authors.initials: K
authors.lastname: Kasama
authors.forename: T
authors.initials: T
authors.lastname: Niwa
authors.forename: K
authors.initials: K
authors.lastname: Harii
authors.forename: M
authors.initials: M
authors.lastname: Iwamori
mesh_terms: D000818:Animals; D002518:Ceramides / Q000737:chemistry / Q000378:metabolism; D002855:Chromatography, Thin Layer; D004817:Epidermis / Q000737:chemistry*; D005227:Fatty Acids / Q000032:analysis*; D006868:Hydrolysis; D051379:Mice; D008815:Mice, Inbred Strains; D009374:Neoplasms, Experimental / Q000139:chemically induced / Q000737:chemistry; D010169:Palmitic Acids / Q000032:analysis; D010212:Papilloma / Q000139:chemically induced / Q000737:chemistry*; D012878:Skin Neoplasms / Q000139:chemically induced / Q000737:chemistry*; D013109:Sphingomyelins / Q000737:chemistry* / Q000378:metabolism
chemical_list: D002518:Ceramides; D005227:Fatty Acids; D010169:Palmitic Acids; D013109:Sphingomyelins; C024707:2-hydroxyhexadecanoic acid