Tissue specific and androgen-regulated expression of human prostate-specific transglutaminase.
Transglutaminases (TGases) are calcium-dependent enzymes catalysing the post-translational cross-linking of proteins. In the prostate at least two TGases are present, the ubiquitously expressed tissue-type TGase (TGC), and a prostate-restricted TGase (TGP). This paper deals with the molecular cloning and characterization of the cDNA encoding the human prostate TGase (hTGP). For this purpose we have screened a human prostate cDNA library with a probe from the active-site region of TGC. The largest isolated cDNA contained an open reading frame encoding a protein of 684 amino acids with a predicted molecular mass of 77 kDa as confirmed by in vitro transcription-translation and subsequent SDS/PAGE. The hTGP gene was tissue-specifically expressed in the prostate, yielding an mRNA of approx. 3.5 kb. Furthermore, a 3-fold androgen-induced upregulation of hTGP mRNA expression has been demonstrated in the recently developed human prostate cancer cell line, PC346C. Other well established human prostate cancer cell lines, LNCaP and PC-3, showed no detectable hTGP mRNA expression on a Northern bolt. The gene coding for prostate TGase was assigned to chromosome 3.
Dubbink
H J
HJ
Department of Urology, Erasmus University, Rotterdam, The Netherlands.
Verkaik
N S
NS
Faber
P W
PW
Trapman
J
J
Schröder
F H
FH
Romijn
J C
JC
D000595:Amino Acid Sequence; D000728:Androgens / Q000378:metabolism*; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D002893:Chromosomes, Human, Pair 3 / Q000235:genetics; D003001:Cloning, Molecular; D006224:Cricetinae; D015342:DNA Probes / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D006822:Hybrid Cells; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D011467:Prostate / Q000201:enzymology*; D011471:Prostatic Neoplasms / Q000201:enzymology / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution; D011503:Transglutaminases / Q000235:genetics / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D000728:Androgens; D015342:DNA Probes; D018076:DNA, Complementary; D011503:Transglutaminases