Isolation and characterization of a human cDNA clone (GCN5L1) homologous to GCN5, a yeast transcription activator.
We have isolated a novel human cDNA with a predicted amino acid sequence homologous to GCN5, a protein considered to be a regulator of transcriptional activation in yeast. This cDNA, termed GCN5L1 (GCN5-like 1), consists of 545 nucleotides including an open reading frame of 378 bp that encodes a 126-amino-acid peptide having 23.5% identity to yeast GCN5. Northern-blot analysis revealed transcription of this gene in all human tissues examined. We isolated a genomic clone corresponding to this cDNA from a human cosmid library and mapped it to chromosome 12q13 --> q14 by fluorescent in situ hybridization (FISH).
Inoue
M
M
Laboratory of Molecular Medicine, University of Tokyo, Japan.
Isomura
M
M
Ikegawa
S
S
Fujiwara
T
T
Shin
S
S
Moriya
H
H
Nakamura
Y
Y
D000595:Amino Acid Sequence; D001483:Base Sequence; D002881:Chromosomes, Human, Pair 12 / Q000235:genetics*; D003001:Cloning, Molecular; D003360:Cosmids; D018076:DNA, Complementary / Q000235:genetics*; D004268:DNA-Binding Proteins; D005656:Fungal Proteins / Q000235:genetics*; D015870:Gene Expression; D015723:Gene Library; D005796:Genes; D005800:Genes, Fungal; D051548:Histone Acetyltransferases; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins; D016366:Open Reading Frames; D011494:Protein Kinases / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D015534:Trans-Activators / Q000235:genetics*; D000099224:p300-CBP-Associated Factor
D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D051548:Histone Acetyltransferases; D009419:Nerve Tissue Proteins; D011494:Protein Kinases; D029701:Saccharomyces cerevisiae Proteins; D015534:Trans-Activators; C104378:BLOC1S1 protein, human; C501392:GCN5 protein, S cerevisiae; D000099224:p300-CBP-Associated Factor