Soluble CD40 ligand induces expression of CD25 and CD23 in resting human tonsillar B lymphocytes.
In this report, we describe the dose-dependent increase in both CD25 and CD23 levels on resting human B cells in response to CD40 ligation, as mediated by soluble CD40 ligand (sCD40L) or anti-CD40 antibody. In combination with interleukin (IL)-4, sCD40L had limited additive effects on CD25 expression, but significantly enhanced CD23 expression on tonsillar B cells. Interferon-gamma (IFN-gamma) exerted no inhibitory effect upon increases in CD25 or CD23 driven by CD40 ligation with sCD40L or anti-CD40 antibody. These data suggest that the induction of CD25 and CD23 genes by IL-4 is mediated, at least in part, by an IFN-gamma-sensitive component, whereas gene activation driven via CD40 ligation involves signaling pathways which are not sensitive to IFN-gamma.
Burlinson
E L
EL
Division of Biochemistry and Molecular Biology, Institute of Biomedical and Life Sciences, University of Glasgow, Scotland.
Graber
P
P
Bonnefoy
J Y
JY
Ozanne
B W
BW
Cushley
W
W
D000911:Antibodies, Monoclonal / Q000494:pharmacology; D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D019013:CD40 Antigens / Q000276:immunology; D023201:CD40 Ligand; D004357:Drug Synergism; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology; D015847:Interleukin-4 / Q000494:pharmacology; D007399:Interphase / Q000187:drug effects / Q000276:immunology; D008024:Ligands; D018655:Lymphocyte Count; D008562:Membrane Glycoproteins / Q000037:antagonists & inhibitors / Q000276:immunology / Q000494:pharmacology*; D014066:Palatine Tonsil / Q000187:drug effects / Q000378:metabolism*; D017455:Receptors, IgE / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000187:drug effects; D015375:Receptors, Interleukin-2 / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000187:drug effects; D012995:Solubility
D000911:Antibodies, Monoclonal; D019013:CD40 Antigens; D008024:Ligands; D008562:Membrane Glycoproteins; D017455:Receptors, IgE; D015375:Receptors, Interleukin-2; D023201:CD40 Ligand; D015847:Interleukin-4; D007371:Interferon-gamma