title: The exon sequence TAGG can inhibit splicing.
abstract: The fibroblast growth factor receptor-2 gene contains a pair of alternative exons, K-SAM and BEK, which are spliced in a cell type specific manner. We have shown previously that a 10 nucleotide sequence within the K-SAM exon exerts a negative effect on K-SAM exon splicing independent of cell type. We demonstrate here that this sequence works autonomously, as it can repress splicing of a heterologous exon, the EIIIb alternative exon of the rat fibronectin gene. By introducing point mutations into the 10 nucleotide sequence, we have shown that the functional portion is limited to 4 nucleotides, TAGG, the dinucleotide AG of which is particularly important. This short sequence may participate in the control of splicing of exons carrying it, provided that they carry weak splice sites.
authors.affiliation: INSERM U211, Institut de Biologie, Nantes, France.
authors.forename: F
authors.initials: F
authors.lastname: Del Gatto
authors.forename: M C
authors.initials: MC
authors.lastname: Gesnel
authors.forename: R
authors.initials: R
authors.lastname: Breathnach
mesh_terms: D000818:Animals; D001483:Base Sequence; D003062:Codon; D005091:Exons; D005353:Fibronectins / Q000235:genetics; D008969:Molecular Sequence Data; D016296:Mutagenesis; D017354:Point Mutation; D012326:RNA Splicing; D051381:Rats; D017468:Receptors, Fibroblast Growth Factor / Q000235:genetics*
chemical_list: D003062:Codon; D005353:Fibronectins; D017468:Receptors, Fibroblast Growth Factor