Cloning, characterization and functional analysis of groEL-like gene from thermophilic cyanobacterium Synechococcus vulcanus, which does not form an operon with groES.
A gene encoding 57 102 Da polypeptide homologous to groEL of Escherichia coli but accompanying no groES, has been cloned and sequenced from a thermophilic cyanobacterium, Synechococcus vulcanus. The amount of the gene transcript increased several folds by heat shock. The gene was expressed as a minor component of two types of HSP60, and designated as groEL2. Although expressed and induced well upon heat shock treatment in the E. coli, introduction of the cloned groEL2 gene of S. vulcanus into an E. coli groEL-less mutant did not result in the complementation of heat sensitivity.
Furuki
M
M
Department of Biochemistry and Molecular Biology, Saitama University, Urawa, Japan.
Tanaka
N
N
Hiyama
T
T
Nakamoto
H
H
D000595:Amino Acid Sequence; D001483:Base Sequence; D018834:Chaperonin 60 / Q000096:biosynthesis*; D003001:Cloning, Molecular; D000458:Cyanobacteria / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000254:growth & development / Q000378:metabolism; D005798:Genes, Bacterial; D006358:Hot Temperature; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D015183:Restriction Mapping; D014158:Transcription, Genetic
D018834:Chaperonin 60