title: Cloning and functional expression of a human Ca2+-permeable cation channel activated by calcium store depletion.
abstract: Depletion of intracellular calcium stores generates a signal that activates Ca2+-permeable channels in the plasma membrane. We have identified a human cDNA, TRPC1A, from a human fetal brain cDNA library. TRPC1A is homologous to the cation channels trp and trpl in Drosophila and is a splice variant of the recently identified cDNA Htrp-1. Expression of TRPC1A in CHO cells induced nonselective cation currents with similar permeabilities for Na+, Ca2+, and Cs+. The currents were activated by intracellular infusion of myo inositol 1,4,5-trisphosphate or thapsigargin. Expression of TRPC1A significantly enhanced increases in the intracellular free calcium concentration induced by Ca2+ restitution after prolonged depletion. Similar results were obtained in Sf9 cells. We conclude that TRPC1A encodes a Ca2+-permeable cation channel activated by depletion of intracellular calcium stores.
authors.affiliation: Institut für Pharmakologie, Freie Universität Berlin, Federal Republic of Germany.
authors.forename: C
authors.initials: C
authors.lastname: Zitt
authors.forename: A
authors.initials: A
authors.lastname: Zobel
authors.forename: A G
authors.initials: AG
authors.lastname: Obukhov
authors.forename: C
authors.initials: C
authors.lastname: Harteneck
authors.forename: F
authors.initials: F
authors.lastname: Kalkbrenner
authors.forename: A
authors.initials: A
authors.lastname: Lückhoff
authors.forename: G
authors.initials: G
authors.lastname: Schultz
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D016466:CHO Cells; D015220:Calcium Channels / Q000235:genetics / Q000502:physiology*; D002412:Cations; D003001:Cloning, Molecular; D006224:Cricetinae; D006801:Humans; D007473:Ion Channels / Q000235:genetics / Q000502:physiology*; D008564:Membrane Potentials / Q000502:physiology; D008969:Molecular Sequence Data; D018408:Patch-Clamp Techniques
chemical_list: D015220:Calcium Channels; D002412:Cations; D007473:Ion Channels