title: NF-kappaB p50 subunit cross-linking to DNA duplexes, containing a monosubstituted pyrophosphate internucleotide bond.
abstract: The new express technique based on the use of BrCN to synthesize DNA duplexes, containing non-substituted or monosubstituted pyrophosphate internucleotide bonds has been proposed. Using this technique, DNA duplexes having modified internucleotide bonds between dT and dC residues in the human NF-kappaB transcription factor recognition sequence in HIV-1 (5'-GGAAAGTCCC-3') have been prepared. We demonstrate that these internucleotide bonds within the recognition site do not prevent the formation of NF-kappaB p50 subunit complex with the corresponding duplexes. The cross-linking of NF-kappaB p50 subunit to the DNA duplex containing a monosubstituted pyrophosphate internucleotide bond has been successfully performed.
authors.affiliation: A.N. Belozersky Institute of Physical and Chemical Biology and Department of Chemistry, Moscow State University, Russia.
authors.forename: E A
authors.initials: EA
authors.lastname: Kubareva
authors.forename: O A
authors.initials: OA
authors.lastname: Fedorova
authors.forename: M B
authors.initials: MB
authors.lastname: Gottikh
authors.forename: H
authors.initials: H
authors.lastname: Tanaka
authors.forename: C
authors.initials: C
authors.lastname: Malvy
authors.forename: Z A
authors.initials: ZA
authors.lastname: Shabarova
mesh_terms: D001482:Base Composition; D001483:Base Sequence; D001665:Binding Sites; D003432:Cross-Linking Reagents; D004247:DNA / Q000378:metabolism*; D004279:DNA, Viral / Q000737:chemistry / Q000378:metabolism*; D011756:Diphosphates; D005982:Glutathione Transferase / Q000302:isolation & purification / Q000378:metabolism; D015497:HIV-1 / Q000235:genetics; D006801:Humans; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D016328:NF-kappa B / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D009838:Oligodeoxyribonucleotides / Q000302:isolation & purification / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D003432:Cross-Linking Reagents; D004279:DNA, Viral; D011756:Diphosphates; D046911:Macromolecular Substances; D016328:NF-kappa B; D009838:Oligodeoxyribonucleotides; D011993:Recombinant Fusion Proteins; D004247:DNA; D005982:Glutathione Transferase