[Comparison of repeat DNA sequences in the Erinaceidae mammal family by restriction analysis].
Hedgehog (Erinaceidae) DNA was digested with teh Sau 96 I, Bsp 143 I, Csp 6 I, Taq I, Hinf I, Msp I, Eco 130I, Bcn I, BsuR I restriction endonucleases. The obtained products were end-labeled and electrophoretically separated in polyacrylamide gel. DNA fragments consisting of highly repetitive genomic sequences were detected as a set of bands corresponding to fragments between 30 and 500 bp in length. Comparison of DNA restriction patterns of the species analyzed revealed the presence of species-specific bands as well as common bands. Phylogenetic trees were constructed by means of the maximum parsimony method and the bootstrap procedure. Our data suggest that hedgehog species from arid areas are clearly distinguished from forest species.
Bannikova
A A
AA
Fedorova
L V
LV
Fedorov
A N
AN
Troitskiĭ
A V
AV
Grechko
V V
VV
Dolgov
V A
VA
Lomov
A A
AA
Mednikov
B M
BM
D000818:Animals; D004247:DNA / Q000378:metabolism; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D006364:Hedgehogs / Q000235:genetics*; D010802:Phylogeny; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D013045:Species Specificity
D004247:DNA; D004262:DNA Restriction Enzymes
Sravnenie povtoriaiushchikhsia posledovatel'nosteĭ DNK mlekopitaiushchikh semeĭstva Erinaceidae metodom restriktaznogo analiza.