Cloning and chromosomal mapping of three novel genes, GPR9, GPR10, and GPR14, encoding receptors related to interleukin 8, neuropeptide Y, and somatostatin receptors.
We employed the polymerase chain reaction and genomic DNA library screening to clone novel human genes, GPR9 and GPR10, and a rat gene, GPR14. GPR9, GPR10, and GPR14 each encode G protein-coupled receptors. GPR10 and GPR14 are intronless within their coding regions, while GPR9 contains at least one intron. The receptor encoded by GPR9 shares the highest identity with human IL-8 receptor type B (38% overall and 53% in the transmembrane regions), followed by IL-8 receptor type A (36% overall and 51% in the transmembrane domains). GPR10 encodes a receptor that shares highest identity with the neuropeptide Y receptor (31% overall and 46% in the transmembrane domains). The receptor encoded by GPR14 shares highest identity with the somatostatin receptor SSTR 4 (27% overall and 41% in the transmembrane domains). Fluorescence in situ hybridization analysis localized GPR9 to chromosome 8p11.2-p12 and GPR10 to chromosome 10q25.3-q26.
Marchese
A
A
Addiction Research Foundation, Toronto, Ontario, Canada.
Heiber
M
M
Nguyen
T
T
Heng
H H
HH
Saldivia
V R
VR
Cheng
R
R
Murphy
P M
PM
Tsui
L C
LC
Shi
X
X
Gregor
P
P
D000595:Amino Acid Sequence; D015703:Antigens, CD / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D015152:Blotting, Northern; D001921:Brain / Q000378:metabolism; D002874:Chromosome Mapping; D002898:Chromosomes, Human, Pair 8; D003001:Cloning, Molecular; D004247:DNA; D015723:Gene Library; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D016209:Interleukin-8 / Q000378:metabolism; D008214:Lymphocytes / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000302:isolation & purification / Q000378:metabolism; D018123:Receptors, Interleukin / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D023062:Receptors, Interleukin-8A; D017476:Receptors, Neuropeptide Y / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D011957:Receptors, Opioid / Q000737:chemistry / Q000235:genetics; D017481:Receptors, Somatostatin / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry; D017386:Sequence Homology, Amino Acid
D015703:Antigens, CD; D016209:Interleukin-8; D012333:RNA, Messenger; D018123:Receptors, Interleukin; D023062:Receptors, Interleukin-8A; D017476:Receptors, Neuropeptide Y; D011957:Receptors, Opioid; D017481:Receptors, Somatostatin; D011994:Recombinant Proteins; D004247:DNA