title: Pax-1 in the development of the cervico-occipital transitional zone.
abstract: The Pax-1 gene has been found to play an important role in the development of the vertebral column. The cervico-occipital transitional zone is a specialized region of the vertebral column, and malformations of this region have frequently been described in humans. The exact embryonic border between head and trunk is a matter of controversy. In order to determine a possible role of Pax-1 in the development of the cervico-occipital transitional zone we studied the expression of this gene in a series of quail embryos and murine fetuses with in situ hybridization and immunohistochemistry. Pax-1 is expressed in all somites of the embryo, including the first five occipital ones. During embryonic days 3-5 the gene is down-regulated in the caudal direction within the first five somites, whereas more caudally Pax-1 is strongly expressed in the cells of the perinotochordal tube. In 5-day-old quail embryos, the cartilaginous anlage of the basioccipital bone has developed and ther is no more expression of Pax-1 in this region. The fusion of the dens axis with the body of the axis also coincides with switching off of the Pax-1 gene. More caudally, the gene is continuously expressed in the intervertebral discs of murine embryos and therefore seems to be important for the process of resegmentation. Quail embryos do not possess permanent intervertebral discs. ¿Hyper-¿ or ¿hyposegmentation¿ defects may be explained by an over- or under-expression of Pax-1 during development. We also reinvestigated the border between the head and trunk in chick embryos by performing homotopical grafting experiments of the 5th somite between chick and quail embryos.
authors.affiliation: Anatomisches Institut der Albert-Ludwigs-Universität, Lehrstuhl II, Freiburg, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Wilting
authors.forename: C
authors.initials: C
authors.lastname: Ebensperger
authors.forename: T S
authors.initials: TS
authors.lastname: Müller
authors.forename: H
authors.initials: H
authors.lastname: Koseki
authors.forename: J
authors.initials: J
authors.lastname: Wallin
authors.forename: B
authors.initials: B
authors.lastname: Christ
mesh_terms: D000818:Animals; D002574:Cervical Vertebrae / Q000737:chemistry* / Q000196:embryology*; D003370:Coturnix; D004268:DNA-Binding Proteins / Q000032:analysis*; D051379:Mice; D009777:Occipital Bone / Q000737:chemistry* / Q000196:embryology*; D051761:Paired Box Transcription Factors; D014157:Transcription Factors / Q000032:analysis*
chemical_list: D004268:DNA-Binding Proteins; D051761:Paired Box Transcription Factors; D014157:Transcription Factors; C070835:PAX1 transcription factor