Assignment of the human alpha-tropomyosin gene TPM4 to band 19p13.1 by fluorescence in situ hybridization.
Sequence-tagged sites (STSs) were developed for the human alpha-tropomyosin gene TPM4. One STS was used to amplify DNA from somatic cell hybrids to localize TPM4 to chromosome 19. The other, a product from a long-range PCR, was used directly as a probe to refine the localization of TPM4 to 19p13.1 by fluorescence in situ hybridization to metaphase chromosome spreads.
Wilton
S D
SD
Australian Neuromuscular Research Institute, QE II Medical Centre, Nedlands, Western Australia, Australia.
Lim
L
L
Dorosz
S D
SD
Gunn
H C
HC
Eyre
H J
HJ
Callen
D F
DF
Laing
N G
NG
D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D002888:Chromosomes, Human, Pair 19 / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D015342:DNA Probes / Q000235:genetics; D006801:Humans; D006822:Hybrid Cells; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D016324:Sequence Tagged Sites; D014335:Tropomyosin / Q000235:genetics*
D017931:DNA Primers; D015342:DNA Probes; D014335:Tropomyosin