title: Cone-associated c-fos gene expression in the light-damaged rat retina.
abstract: OBJECTIVE
  To examine whether light-controlled c-fos gene expression is mediated by a cone specific pathway in the rat retina.
  
  
  METHODS
  To produce a cone-rich retina, rats were placed under continuous lighting for 21 days. The illuminance in the cages ranged from 1200 to 2000 lux. The presence of cones was determined by peanut agglutinin and rods by the monoclonal antibody 1-E7. The presence of c-fos transcripts in the retinas was analyzed by in situ hybridization using single-stranded RNA probes.
  
  
  RESULTS
  After long-term exposure to continuous light, the 1-E7 immunoreactivity was not detected in the outer nuclear layer. The cone photoreceptors and cells in the ganglion cell (GCL) and inner nuclear layers (INL) survived. In normal retinas, there was strong hybridization for c-fos expression in the GCL and the INL 30 minutes after the onset of the light cycle. Light-damaged retinas also showed hybridization in the GCL but not in the INL under the same light regime.
  
  
  CONCLUSIONS
  These results suggest that c-fos gene expression can be controlled through a cone specific pathway in the retina.
authors.affiliation: Department of Ophthalmology, Hokkaido University School of Medicine, Sapporo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Harada
authors.forename: J
authors.initials: J
authors.lastname: Imaki
authors.forename: K
authors.initials: K
authors.lastname: Ohki
authors.forename: K
authors.initials: K
authors.lastname: Ono
authors.forename: T
authors.initials: T
authors.lastname: Ohashi
authors.forename: H
authors.initials: H
authors.lastname: Matsuda
authors.forename: K
authors.initials: K
authors.lastname: Yoshida
mesh_terms: D000818:Animals; D005786:Gene Expression Regulation; D016762:Genes, fos / Q000235:genetics; D007124:Immunoenzyme Techniques; D017403:In Situ Hybridization; D008027:Light / Q000009:adverse effects*; D008297:Male; D010786:Photoreceptor Cells / Q000378:metabolism; D016760:Proto-Oncogene Proteins c-fos / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011833:Radiation Injuries, Experimental / Q000209:etiology / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D011916:Rats, Inbred F344; D012160:Retina / Q000378:metabolism* / Q000473:pathology / Q000528:radiation effects; D017949:Retinal Cone Photoreceptor Cells / Q000378:metabolism*; D012162:Retinal Degeneration / Q000209:etiology / Q000378:metabolism* / Q000473:pathology
chemical_list: D016760:Proto-Oncogene Proteins c-fos; D012333:RNA, Messenger