title: Cyclooxygenase-2 overexpression and tumor formation are blocked by sulindac in a murine model of familial adenomatous polyposis.
abstract: Inducible cyclooxygenase (Cox-2), also known as prostaglandin H synthase 2 (PGH-2) is a key enzyme in the formation of prostaglandins and thromboxanes. Cox-2 is the product of an immediate-early gene that is expressed in response to growth factors, tumor promoters, or cytokines. Overexpression of Cox-2 is associated with both human colon cancers and suppression of apoptosis in cultured epithelia] cells, an activity that is reversed by the nonsteroidal anti-inflammatory drug, sulindac sulfide. To address the relationship between Cox-2, apoptosis, and tumor development in vivo, we studied C57BL/6J-Min/+(Min) mice, a strain containing a fully penetrant dominant mutation in the Apc gene, leading to the development of gastrointestinal adenomas by 110 days of age. Min mice were fed AIN-76A chow diet and given sulindac (0.5 +/- 0.1 mg/day) in drinking water. Control Min mice and homozygous C57BL/6J-+/+ normal littermates lacking the Apc mutation (+/+) were fed AIN-76A diet and given tap water to drink. At 110 days of age, all mice were sacrificed, and their intestinal tracts were examined. Control Min mice had 11.9 +/- 7.8 tumors per mouse compared to 0.1 +/- 0.1 tumors for sulindac-treated Min mice. As expected, +/+ littermates had no macroscopic tumors. Examination of histologically normal-appearing small bowel from Min animals revealed increased amounts of Cox-2 and prostaglandin E(2) compared to +/+ littermates. Using two different in situ techniques, terminal transferase-mediated dUTP nick end labeling and a direct immunoperoxidase method, Min animals also demonstrated a 27-47% decrease in enterocyte apoptosis compared to +/+ animals. Treatment with sulindac not only inhibited tumor formation but decreased small bowel Cox-2 and prostaglandin E(2) to baseline and restored normal levels of apoptosis. These data suggest that overexpression of Cox-2 is associated with tumorigenesis in the gastrointestinal epithelium, and that both are inhibited by sulindac administration.
authors.affiliation: Strang Cancer Prevention Center, New York, 10021, USA.
authors.forename: S K
authors.initials: SK
authors.lastname: Boolbol
authors.forename: A J
authors.initials: AJ
authors.lastname: Dannenberg
authors.forename: A
authors.initials: A
authors.lastname: Chadburn
authors.forename: C
authors.initials: C
authors.lastname: Martucci
authors.forename: X J
authors.initials: XJ
authors.lastname: Guo
authors.forename: J T
authors.initials: JT
authors.lastname: Ramonetti
authors.forename: M
authors.initials: M
authors.lastname: Abreu-Goris
authors.forename: H L
authors.initials: HL
authors.lastname: Newmark
authors.forename: M L
authors.initials: ML
authors.lastname: Lipkin
authors.forename: J J
authors.initials: JJ
authors.lastname: DeCosse
authors.forename: M M
authors.initials: MM
authors.lastname: Bertagnolli
mesh_terms: D011125:Adenomatous Polyposis Coli / Q000188:drug therapy*; D000818:Animals; D017209:Apoptosis; D001483:Base Sequence; D051546:Cyclooxygenase 2; D052246:Cyclooxygenase 2 Inhibitors; D016861:Cyclooxygenase Inhibitors / Q000627:therapeutic use*; D016207:Cytokines / Q000235:genetics; D017931:DNA Primers / Q000737:chemistry; D004847:Epithelial Cells; D005260:Female; D015870:Gene Expression; D007413:Intestinal Mucosa / Q000166:cytology; D007527:Isoenzymes / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D008969:Molecular Sequence Data; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D013467:Sulindac / Q000627:therapeutic use*
chemical_list: D052246:Cyclooxygenase 2 Inhibitors; D016861:Cyclooxygenase Inhibitors; D016207:Cytokines; D017931:DNA Primers; D007527:Isoenzymes; D012333:RNA, Messenger; D013467:Sulindac; D051546:Cyclooxygenase 2; D011451:Prostaglandin-Endoperoxide Synthases