title: Influence of human papillomavirus type 16 gene expression on in vitro differentiation of the human teratocarcinoma cell line 2102Ep.
abstract: Human papillomaviruses (HPVs) are known to infect human keratinocytes and cause alterations in epithelial differentiation. We showed in this study that expression of the HPV-16 genome was able to interfere with the in vitro differentiation of a human simple-epithelial cell type, the 2102Ep teratocarcinoma cell line. Stable HPV-16 genome-expressing 2102Ep cell lines were generated, and subsequent alterations in differentiation were analyzed in comparison with parental 2102Ep cells. We found that in 2102Ep cells phorbol ester-induced differentiation led to changes in the expression of SSEA antigens, whereas in HPV-transfected cell lines only minor changes were observed.
authors.affiliation: Angewandte Tumorvirologie, Deutsches Krebsforschungszentrum, Heidelberg, Germany.
authors.forename: H
authors.initials: H
authors.lastname: Stöppler
authors.forename: T
authors.initials: T
authors.lastname: Kirchhoff
authors.forename: J
authors.initials: J
authors.lastname: Kartenbeck
authors.forename: L
authors.initials: L
authors.lastname: Gissmann
authors.forename: A
authors.initials: A
authors.lastname: Alonso
mesh_terms: D000951:Antigens, Neoplasm / Q000096:biosynthesis; D015295:Antigens, Tumor-Associated, Carbohydrate; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D004279:DNA, Viral / Q000235:genetics*; D015870:Gene Expression; D005814:Genes, Viral; D006028:Glycosphingolipids / Q000096:biosynthesis; D006801:Humans; D016256:Lewis X Antigen / Q000096:biosynthesis; D008297:Male; D027383:Papillomaviridae / Q000235:genetics* / Q000378:metabolism; D055746:Stage-Specific Embryonic Antigens; D018243:Teratocarcinoma / Q000378:metabolism / Q000473:pathology* / Q000821:virology*; D013736:Testicular Neoplasms / Q000378:metabolism / Q000473:pathology* / Q000821:virology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014162:Transfection; D014407:Tumor Cells, Cultured
chemical_list: D000951:Antigens, Neoplasm; D015295:Antigens, Tumor-Associated, Carbohydrate; D004279:DNA, Viral; D006028:Glycosphingolipids; D016256:Lewis X Antigen; D055746:Stage-Specific Embryonic Antigens; C052924:stage-specific embryonic antigen-3; D013755:Tetradecanoylphorbol Acetate