title: RNA-mediated resistance with nonstructural genes from the tobacco etch virus genome.
abstract: Sense RNA-mediated virus resistance has been described for transgenic plants expressing potyviral capsid protein sequences. This study was undertaken to determine if expression of other viral sequences could induce this type of virus resistance. Plants showing highly resistant or 'recovery' phenotypes were generated by expressing the tobacco etch virus (TEV) 6 kDa/21 kDa reading frames. Expression of translatable or untranslatable versions of this TEV sequence produced resistant lines. Highly resistant and recovery phenotype plants expressing TEV coat protein sequences. High transcription rates with low steady-state levels of the transgene transcript generally correlated with resistance. During recovery and induction of the resistance, RNA and protein steady-state levels decreased 5- to 20-fold, while transcription of the transgene continued at a similar level. A posttranscriptional, cellular system eliminating sequences contained in the transgene transcript and viral genome would be consistent with the results.
authors.affiliation: Department of Microbiology, Oregon State University, Corvallis 97331-3804, USA.
authors.forename: S
authors.initials: S
authors.lastname: Swaney
authors.forename: H
authors.initials: H
authors.lastname: Powers
authors.forename: J
authors.initials: J
authors.lastname: Goodwin
authors.forename: L S
authors.initials: LS
authors.lastname: Rosales
authors.forename: W G
authors.initials: WG
authors.lastname: Dougherty
mesh_terms: D001483:Base Sequence; D005814:Genes, Viral; D007113:Immunity, Innate / Q000235:genetics; D008969:Molecular Sequence Data; D010641:Phenotype; D010935:Plant Diseases / Q000821:virology*; D030821:Plants, Genetically Modified; D010947:Plants, Toxic; D017800:Potyvirus / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics*; D012641:Selection, Genetic; D014026:Nicotiana / Q000235:genetics / Q000821:virology*; D014158:Transcription, Genetic
chemical_list: D012367:RNA, Viral