title: Human genomic characterization of a novel locus-specific repetitive sequence.
abstract: A novel human chromosome locus-specific repetitive sequence was identified and characterized using arbitrary PCR. The repeat monomer consensus sequence is 100 bp long, and there are a minimum of 140 to 160 copies of the repetitive sequence per haploid human genome. The repetitive sequence is highly clustered on 20q12 within a 200- to 400-kb region. The highly polymorphic repeat array is inherited in a stable Mendelian fashion. Hybridization analysis revealed detectable conservation of the repeated element only among hominoids and Old World monkeys, where repeat arrangements are also polymorphic.
authors.affiliation: BIOS Laboratories, Inc., New Haven, Connecticut 06511, USA.
authors.forename: W L
authors.initials: WL
authors.lastname: Li
authors.forename: C S
authors.initials: CS
authors.lastname: Huckaby
authors.forename: R E
authors.initials: RE
authors.lastname: Kouri
authors.forename: G
authors.initials: G
authors.lastname: Ruano
mesh_terms: D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002520:Cercopithecidae / Q000235:genetics; D002874:Chromosome Mapping; D016384:Consensus Sequence; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D015894:Genome, Human; D015186:Hominidae / Q000235:genetics*; D006801:Humans; D006822:Hybrid Cells; D008297:Male; D008969:Molecular Sequence Data; D010375:Pedigree; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D019105:Random Amplified Polymorphic DNA Technique; D012091:Repetitive Sequences, Nucleic Acid; D012689:Sequence Homology, Nucleic Acid
chemical_list: D004247:DNA