Transcriptional regulation of the MHC class I HLA-A11 promoter by the zinc finger protein ZFX.
Regulation of the human MHC class I HLA-A11 promoter is governed by a complex array of regulatory elements. One of these elements, shown here to be critical for the transcriptional activity of the promoter, was used to screen a lambda gt11 library and allowed the identification of a cDNA which coded for the zinc finger protein ZFX. ZFX was shown to bind the sequences AGGGCCCCA and AGGCCCCGA, located respectively at positions -271 to -263 and -242 to -234 of the HLA-A11 promoter, with similar affinities through its three C-terminal zinc fingers. ZFX575, a short isoform of ZFX, activates transcription from the HLA-All promoter in a Leydig cell line.
L'Haridon
M
M
INSERM U93, Centre Hayem, Paris, France.
Paul
P
P
Xerri
J G
JG
Dastot
H
H
Dolliger
C
C
Schmid
M
M
de Angelis
N
N
Grollet
L
L
Sigaux
F
F
Degos
L
L
Gazin
C
C
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002460:Cell Line; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism* / Q000494:pharmacology; D005786:Gene Expression Regulation; D015234:HLA-A Antigens / Q000235:genetics*; D059870:HLA-A11 Antigen; D006367:HeLa Cells; D006801:Humans; D051741:Kruppel-Like Transcription Factors; D007985:Leydig Cells / Q000378:metabolism; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000494:pharmacology; D014157:Transcription Factors; D014158:Transcription, Genetic / Q000187:drug effects; D014162:Transfection; D016335:Zinc Fingers
D004268:DNA-Binding Proteins; D015234:HLA-A Antigens; D059870:HLA-A11 Antigen; D051741:Kruppel-Like Transcription Factors; D011994:Recombinant Proteins; D014157:Transcription Factors; C062076:zinc finger protein, X-linked; D004247:DNA