title: Aprotinin inhibits unspecific degradation of collagen in rat and human pancreas.
abstract: CONCLUSIONS
  Addition of aprotinin in human and rat pancreatic extracts inactivates nonspecific proteases that completely degrade collagen.
  
  
  BACKGROUND
  We sought to clarify the relative roles of collagenase and nonspecific proteases in the breakdown of collagen by the pancreas.
  
  
  METHODS
  The degradation of [3H] collagen fibrils by pancreatic extracts to small fragments of low molecular weight was determined by SDS-electrophoresis and autoradiography. Aprotinin (0.14 mg/mL) was added to inhibit nonspecific protease activity.
  
  
  RESULTS
  Rat and human pancreas extracts contained a high collagenolytic activity that was demonstrated to be the result of the combined action of collagenase and other pancreatic proteases. Seventy percent of the total collagenolytic activity in rat pancreas extracts was inhibited by aprotinin. The same aprotinin concentration had no effect on two commercially available collagenases. The electrophoretic pattern obtained from [3H] collagen treated with rat and human pancreatic extracts containing aprotinin confirmed the presence of a true specific collagenase in the pancreas.
authors.affiliation: Gastroenterology Service, Hospital Clínic i Provincial, Barcelona, Spain.
authors.forename: J M
authors.initials: JM
authors.lastname: López
authors.forename: R
authors.initials: R
authors.lastname: Valderrama
authors.forename: S
authors.initials: S
authors.lastname: Navarro
authors.forename: S
authors.initials: S
authors.lastname: Imperial
mesh_terms: D000818:Animals; D007611:Aprotinin / Q000494:pharmacology*; D001345:Autoradiography; D003094:Collagen / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008297:Male; D061965:Matrix Metalloproteinase Inhibitors; D010179:Pancreas / Q000187:drug effects* / Q000201:enzymology*; D051381:Rats; D015842:Serine Proteinase Inhibitors / Q000494:pharmacology*
chemical_list: D061965:Matrix Metalloproteinase Inhibitors; D015842:Serine Proteinase Inhibitors; D003094:Collagen; D007611:Aprotinin