Four dually resistant human cytomegalovirus strains from AIDS patients: single mutations in UL97 and UL54 open reading frames are responsible for ganciclovir- and foscarnet-specific resistance, respectively.
Four human cytomegalovirus strains with double resistance to both ganciclovir and foscarnet were recovered from acquired immunodeficiency syndrome (AIDS) patients. The four isolates were genetically unrelated and each of them consisted of a single viral population. In addition, for each isolate, UL97 and UL54 open reading frames sequencing showed a single aminoacid change in a conserved domain of each of the two genes.
Baldanti
F
F
Viral Diagnostic Service IRCCS Policlinico S. Matteo, Pavia, Italy.
Sarasini
A
A
Silini
E
E
Barbi
M
M
Lazzarin
A
A
Biron
K K
KK
Gerna
G
G
D017088:AIDS-Related Opportunistic Infections / Q000188:drug therapy* / Q000821:virology; D000998:Antiviral Agents / Q000494:pharmacology / Q000627:therapeutic use; D003587:Cytomegalovirus / Q000187:drug effects / Q000235:genetics*; D003586:Cytomegalovirus Infections / Q000188:drug therapy* / Q000821:virology; D004352:Drug Resistance, Microbial / Q000235:genetics*; D018432:Drug Resistance, Multiple / Q000235:genetics*; D017245:Foscarnet / Q000494:pharmacology / Q000627:therapeutic use; D015774:Ganciclovir / Q000494:pharmacology / Q000627:therapeutic use; D005814:Genes, Viral / Q000235:genetics*; D006801:Humans; D009154:Mutation; D016133:Polymerase Chain Reaction; D014759:Viral Envelope Proteins / Q000235:genetics
D000998:Antiviral Agents; D014759:Viral Envelope Proteins; D017245:Foscarnet; D015774:Ganciclovir