Natriuretic peptide receptors on human trabecular meshwork cells.
The effects of natriuretic peptides on cGMP formation and [125I]ANP binding in human trabecular meshwork cells were investigated. CNP at 1 microM stimulated cGMP formation approximately 18-25 fold, with a half maximal effective concentration approximately 20-30nM. BNP at 1 microM stimulated approximately 7 fold, while ANP stimulated cGMP formation 2-fold at 1 microM but had little or no effect at concentrations below 1 microM. Displacement binding of [125I]ANP to intact TM cells in the presence of unlabeled ANP indicated a single binding site with a dissociation constant approximately 0.15nM.c-ANP, which binds specifically to natriuretic peptide C receptors, displaced > 95% [125I]ANP binding to surface receptor sites with a half-maximal effective concentration comparable to that of ANP or BNP. c-ANP had no inhibitory effect on CNP stimulation of cGMP formation. The data suggest that human TM cells possess natriuretic peptide B receptors as the primary guanylyl cyclase-containing subtype and C receptors as the numerically predominant subtype of natriuretic peptide receptors.
Chang
A T
AT
Department of Ophthalmology, University of California, San Francisco 94143, USA.
Polansky
J R
JR
Crook
R B
RB
D000328:Adult; D009320:Atrial Natriuretic Factor / Q000378:metabolism / Q000494:pharmacology; D001667:Binding, Competitive; D019208:Brain-Derived Neurotrophic Factor / Q000378:metabolism / Q000494:pharmacology; D002478:Cells, Cultured; D006162:Guanylate Cyclase / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D020098:Natriuretic Peptide, C-Type; D011506:Proteins / Q000378:metabolism / Q000494:pharmacology; D020801:Receptor, Ciliary Neurotrophic Factor; D017461:Receptors, Atrial Natriuretic Factor / Q000378:metabolism*; D017475:Receptors, Nerve Growth Factor / Q000378:metabolism*; D014129:Trabecular Meshwork / Q000166:cytology / Q000378:metabolism*
D019208:Brain-Derived Neurotrophic Factor; D011506:Proteins; D020801:Receptor, Ciliary Neurotrophic Factor; D017475:Receptors, Nerve Growth Factor; D020098:Natriuretic Peptide, C-Type; D009320:Atrial Natriuretic Factor; D006162:Guanylate Cyclase; D017461:Receptors, Atrial Natriuretic Factor; C073616:atrial natriuretic factor receptor C