title: Dibutyl phthalate purged autologous bone marrow transplant in the treatment of leukemia.
abstract: It has been proved that di-N-butyl phthalate (DBP) is singular in killing leukemic cells selectively or accelerating the deterioration of residual leukemic cells in long-term marrow culture in vitro. Based on this principle, the DBP-purged autologous bone marrow transplant has been applied to the treatment of a group of 14 patients suffering from acute nonlymphocytic leukemia. After 5-10 days of in vitro co-culture of marrow cells with DBP at a concentration of 50 micrograms/ml, the recovery of total nucleated cells and the amount of CFU-GM were 67.5% and 68.1%, respectively. In all patients, the reconstitution of hematopoiesis was observed after pre-conditioning and transfusion of purged marrow cells. Among these, two patients had a relapse, two patients died from complications of transplant, one patient died from non-leukemic disease, and the others are all alive and free of disease; the mean survival time as calculated recently was 15 months. These preliminary clinical data support that marrow culture in the presence of DBP is a safe and effective measure for treating leukemia in purged autologous bone marrow transplant.
authors.affiliation: Department of Experimental Hematology, Beijing Institute of Radiation Medicine, People's Republic of China.
authors.forename: L
authors.initials: L
authors.lastname: Wang
authors.forename: C
authors.initials: C
authors.lastname: Wu
authors.forename: X
authors.initials: X
authors.lastname: Pei
authors.forename: L
authors.initials: L
authors.lastname: Xu
authors.forename: J
authors.initials: J
authors.lastname: Cao
authors.forename: H
authors.initials: H
authors.lastname: Xue
authors.forename: K
authors.initials: K
authors.lastname: Yang
authors.forename: A
authors.initials: A
authors.lastname: Teng
authors.forename: S
authors.initials: S
authors.lastname: Ji
mesh_terms: D000293:Adolescent; D000328:Adult; D016465:Bone Marrow Purging; D016026:Bone Marrow Transplantation; D002648:Child; D003993:Dibutyl Phthalate / Q000494:pharmacology*; D018572:Disease-Free Survival; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D015470:Leukemia, Myeloid, Acute / Q000628:therapy*; D008297:Male; D014182:Transplantation, Autologous
chemical_list: D003993:Dibutyl Phthalate