title: The association between lung innate immunity and differential airway antigen-specific immune responses.
abstract: The mechanisms involved in the differential regulation of airway immune responses in atopic versus non-atopic individuals are poorly understood. In this study, the association between non-specific immunity and the differential airway antigen-specific immune responses was examined in a murine model. The disparity in antigen-specific IgE and IgG2a productions between the two strains of mice was observed to be significant. C57BL/6J mice were much more efficient than BALB/cJ mice in making IgE antibody to inhaled ovalbumin (OVA) antigen. On the contrary, BALB/cJ mice did make more IgG2a antibodies than C57BL/6J mice to inhaled OVA. These findings suggest that in C57BL/6J mouse strain a predominant Th2 type of immune response develops in response to inhaled OVA antigen. In contrast, BALB/cJ mice mount a Th1 type of immune response to aerosolized OVA antigen. Furthermore, after lipopolysaccharide (LPS) stimulation, the IL-12 mRNA expression of lung-derived cells from BALB/cJ mice was higher than that from C57BL/6J cells. However, the lung-derived cells of C57BL/6J mice stimulated by LPS produced higher levels of IL-10 and prostaglandin E2 than BALB/cJ lung-derived cells did. Therefore, our study demonstrated that the difference of lung-derived cells in their ability to produce cytokine and prostaglandin between BALB/cJ and C57BL/6J mice correlates well with the type of the airway antigen-specific immune effector functions.
authors.affiliation: Graduate Institutes of Microbiology and Immunology, College of Medicine, National Taiwan University, Taipei, ROC.
authors.forename: J Y
authors.initials: JY
authors.lastname: Lin
authors.forename: L F
authors.initials: LF
authors.lastname: Wang
authors.forename: R H
authors.initials: RH
authors.lastname: Lin
mesh_terms: D000818:Animals; D001483:Base Sequence; D001980:Bronchi / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000502:physiology*; D015232:Dinoprostone / Q000096:biosynthesis; D005260:Female; D006969:Hypersensitivity, Immediate; D007113:Immunity, Innate; D007073:Immunoglobulin E / Q000096:biosynthesis; D007074:Immunoglobulin G / Q000096:biosynthesis; D016753:Interleukin-10 / Q000096:biosynthesis; D018664:Interleukin-12 / Q000096:biosynthesis; D008070:Lipopolysaccharides / Q000276:immunology; D008168:Lung / Q000166:cytology / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D010063:Ovum / Q000276:immunology; D016176:T-Lymphocyte Subsets / Q000502:physiology*; D018417:Th1 Cells / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology
chemical_list: D007074:Immunoglobulin G; D008070:Lipopolysaccharides; D016753:Interleukin-10; D018664:Interleukin-12; D007073:Immunoglobulin E; D015232:Dinoprostone