title: Low temperature storage and grafting of human ovarian tissue.
abstract: Ovarian tissue storage at low temperatures is a promising new method for protecting young cancer patients from the sterilizing effects of chemotherapy and/or radiotherapy. Tissue can be stored and returned to the body in due course as a thin cortical graft since the primordial follicles are distributed peripherally and are relatively resistant to ischaemia. Slices of tissue donated by healthy patients were trimmed to a uniform size and preserved by slow freezing to liquid nitrogen temperatures for up to 2 months in one of the following cryoprotectants: dimethylsulphoxide, ethylene glycol, glycerol, propylene glycol. Their viability was assessed by counting follicles in histological sections 18 days after grafting under the kidney capsules of severe combined immunodeficiency (SCID) mice, and the results were expressed as percentages of the numbers in comparable pieces of ungrafted tissue. While only 10% of the total number of follicles was found in the glycerol group compared to controls, significantly higher percentages (44-84%) survived cryopreservation in the other media. The tissues were sterile when frozen and thawed without a cryoprotectant. These results suggest that if comparable results could be achieved by autografting, a patient's fertility should be safeguarded from cytotoxic treatment.
authors.affiliation: Division of Obstetrics and Gynaecology, School of Clinical Medicine, University of Leeds, Clarendon Wing, Leeds General Infirmary, Leeds LS2 9NS, UK.
authors.forename: H
authors.initials: H
authors.lastname: Newton
authors.forename: Y
authors.initials: Y
authors.lastname: Aubard
authors.forename: A
authors.initials: A
authors.lastname: Rutherford
authors.forename: V
authors.initials: V
authors.lastname: Sharma
authors.forename: R
authors.initials: R
authors.lastname: Gosden
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D000970:Antineoplastic Agents / Q000009:adverse effects; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents; D005069:Evaluation Studies as Topic; D005260:Female; D006801:Humans; D007247:Infertility, Female / Q000209:etiology / Q000628:therapy; D051379:Mice; D016513:Mice, SCID; D009369:Neoplasms / Q000188:drug therapy / Q000532:radiotherapy; D010053:Ovary / Q000033:anatomy & histology / Q000637:transplantation*; D011878:Radiotherapy / Q000009:adverse effects; D014182:Transplantation, Autologous; D014183:Transplantation, Heterologous
chemical_list: D000970:Antineoplastic Agents; D003451:Cryoprotective Agents