title: Engagement of adhesion molecules (CD18, CD11a, CD45, CD44, and CD58) enhances human immunodeficiency virus type 1 replication in monocytic cells through a tumor necrosis factor-modulated pathway.
abstract: Engagement of monocytic cell membrane proteins was shown to enhance human immunodeficiency virus type 1 (HIV-1) replication in monocytic cells. Cross-linking of CD18, CD11a, or CD45 by immobilized antibodies produced up to an 11-fold enhancement of HIV-1 release in the OM10.1 monocytic cell line in a tumor necrosis factor-alpha (TNF-alpha)-dependent manner. In addition, adhesion of OM10.1 cells to immobilized intercellular adhesion molecule-1 (ligand for CD18/CD11a) induced similar TNF-alpha-dependent enhancement of HIV-1 replication. After phenotypic differentiation of OM10.1 cells, engagement of cell membrane proteins CD18, CD11a, CD44, CD45, or CD58 by soluble antibodies enhanced HIV-1 replication in a TNF-alpha-dependent manner. These data suggest that cross-linkage of monocytic cell membrane proteins during cell-cell interaction and specifically during antigen presentation to CD4 T cells may enhance HIV-1 replication, facilitating infection of adjacent cells.
authors.affiliation: Division of Infectious Diseases, St. George's Hospital Medical School, London, United Kingdom.
authors.forename: R J
authors.initials: RJ
authors.lastname: Shattock
authors.forename: G P
authors.initials: GP
authors.lastname: Rizzardi
authors.forename: P
authors.initials: P
authors.lastname: Hayes
authors.forename: G E
authors.initials: GE
authors.lastname: Griffin
mesh_terms: D015703:Antigens, CD / Q000502:physiology*; D018845:CD11 Antigens / Q000502:physiology; D018821:CD18 Antigens / Q000502:physiology; D018968:CD58 Antigens / Q000502:physiology; D015815:Cell Adhesion Molecules / Q000502:physiology*; D002478:Cells, Cultured; D005434:Flow Cytometry; D015497:HIV-1; D018960:Hyaluronan Receptors / Q000502:physiology; D007375:Interleukin-1 / Q000502:physiology; D017493:Leukocyte Common Antigens / Q000502:physiology; D009000:Monocytes / Q000821:virology*; D054562:Protein Tyrosine Phosphatase, Non-Receptor Type 1; D014409:Tumor Necrosis Factor-alpha / Q000502:physiology*; D014779:Virus Replication
chemical_list: D015703:Antigens, CD; D018845:CD11 Antigens; D018821:CD18 Antigens; D018968:CD58 Antigens; D015815:Cell Adhesion Molecules; D018960:Hyaluronan Receptors; D007375:Interleukin-1; D014409:Tumor Necrosis Factor-alpha; D017493:Leukocyte Common Antigens; D054562:Protein Tyrosine Phosphatase, Non-Receptor Type 1