title: [A method for differential cytolysis in the molecular genetic expert identification of material evidence: problems in optimizing the procedure].
abstract: The authors discuss the problem of selective derivation of the genetic material of spermatozoa for molecular genetic identification from mixed biological traces containing sperm on material evidence. Possible methods of improving the efficacy of differential lysis of cells present in mixed traces are analyzed. Effects of some routinely used extractants on biological substrata, most often contaminating the sperm in expert material, have been studied, and conditions for their most complete elimination from objects of investigation optimized.
authors.forename: L I
authors.initials: LI
authors.lastname: Gyské
authors.forename: P L
authors.initials: PL
authors.lastname: Ivanov
mesh_terms: D002458:Cell Fractionation / Q000379:methods; D004247:DNA / Q000032:analysis / Q000302:isolation & purification; D004587:Electrophoresis, Agar Gel; D005104:Expert Testimony / Q000379:methods* / Q000592:standards; D005554:Forensic Medicine / Q000379:methods* / Q000592:standards / Q000706:statistics & numerical data; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000592:standards / Q000706:statistics & numerical data; D012044:Regression Analysis; D012426:Russia; D013094:Spermatozoa / Q000737:chemistry / Q000648:ultrastructure
chemical_list: D004247:DNA
vernacular_title: Metod differentsial'nogo lizisa kletok v molekuliarno-geneticheskoĭ identifikatsionnoĭ ékspertize veshchestvennykh dokazatel'stv: voprosy optimizatsii protsedury.