Major differences between the rrnA operons of two strains of Agrobacterium vitis.
The sequence of the rrnA operon and its flanking regions was determined for the Agrobacterium vitis type strain NCPPB3554. Compared to the earlier obtained rrnA sequence of A. vitis strain S4, several important differences were noted: the sequences diverged at the 5'-flanking region, within the 16S-23S intergenic region, and within the 23S rRNA sequence. The B8 stem-loop structure at the 5'-end of the 23S rRNA of strain NCPPB3554 was 142 nt shorter than that of strain S4. These findings have important consequences for the use of ribosomal RNA gene sequences in phylogenetic comparisons.
Otten
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Institute of Plant Molecular Biology of the C. N. R. S., Rue du Général Zimmer 12, F-67084 Strasbourg Cedex, France.
De Ruffray
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D001483:Base Sequence; D008969:Molecular Sequence Data; D012335:RNA, Ribosomal / Q000032:analysis; D012231:Rhizobium / Q000235:genetics*; D012407:rRNA Operon
D012335:RNA, Ribosomal