Terminal long tandem repeats in chromosomes form Chironomus pallidivittatus.
We provide evidence that a chromosome end in the dipteran Chironomus pallidivittatus contains 340-bp tandem repeats reaching the extreme terminus of the chromosome. After adding synthetic oligonucleotide tails to DNA extracted from the microdissected right end of the fourth chromosome, we could demonstrate that the blocks of repeats were tailed at only one end, the chromosome terminus, the interior of the arrays being unavailable for tailing. Using PCR, we furthermore showed that the added tails were connected to 340-bp repeat DNA directly, i.e., without intervening DNA of any other kind. The tailed repeats belong to a subfamily previously known to be the most peripheral one of the different types of 340-bp units. Using plasmid controls, we could also make certain that we did not amplify rare or nonrepresentative DNA termini.
Löpez
C C
CC
Department of Genetics, Lund University, Sweden.
Nielsen
L
L
Edström
J E
JE
D000818:Animals; D001482:Base Composition; D001483:Base Sequence; D002683:Chironomidae / Q000235:genetics*; D002875:Chromosomes / Q000737:chemistry*; D003001:Cloning, Molecular; D016384:Consensus Sequence; D004247:DNA / Q000737:chemistry*; D017931:DNA Primers; D008969:Molecular Sequence Data; D010957:Plasmids; D011089:Polydeoxyribonucleotides; D016133:Polymerase Chain Reaction; D012091:Repetitive Sequences, Nucleic Acid
D017931:DNA Primers; D011089:Polydeoxyribonucleotides; D004247:DNA