[Chromatographic mass spectrometry detection of microorganisms in anaerobic infectious processes].
The selective ion assay (mass-fragmentography) was used to detect some species of microbes in the human biological fluids (urea, pus discharge, pleural fluid, and sperm). This technique is highly sensitive and selective and prompt-to-use. Examinations of the chemical composition of pure microbial cultures determined structural marker substances from a number of fatty acids, hydroxy acids, and aldehydes of the cellular membrane and lipopolysaccharides. The assay can detect microorganisms typical of infectious processes within hours if the bacteria amount to more than 10,000 cells in the culture sample.
Osipov
G A
GA
Demina
A M
AM
D001421:Bacteria, Anaerobic / Q000302:isolation & purification*; D001424:Bacterial Infections / Q000175:diagnosis* / Q000382:microbiology; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D008297:Male; D010996:Pleural Effusion / Q000382:microbiology; D012661:Semen / Q000382:microbiology; D013492:Suppuration / Q000382:microbiology; D014556:Urine / Q000382:microbiology
Khromato-mass-spektrometricheskoe obnaruzhenie mikroorganizmov pri anaérobnykh infektsionnykh protsessakh.