title: Ocular surface upregulation of intercellular adhesive molecule-1 (ICAM-1) by local interferon-gamma (IFN-gamma) in the rat.
abstract: Inflammatory mediators such as interferon-gamma (IFN-gamma) are known to induce the expression of class II HLA (HLA-DR) and intercellular adhesion molecule 1 (ICAM-1) in a variety of cell types including epithelial cells. The coexpression of ICAM-1 and HLA-DR has been implicated in the pathogenesis of immune mediated ocular disease. We investigated the expression of ICAM-1 on the ocular surface of the rat eye following subconjunctival administration of IFN-gamma. A dose response study was performed using 100, 1,000 and 10,000 IU IFN-gamma/dose. The presence of ICAM-1 was determined using a standard immuno-peroxidase staining technique. The cornea, limbus, and conjunctiva were evaluated. The degree of reaction product in each area was graded by a masked observer. We found constitutive expression of ICAM-1 on the conjunctiva but not on the cornea. There was a significant relationship between the dose of IFN-gamma and the intensity of ICAM-1 staining on the conjunctiva (p < or = 0.05) and on the limbus (p < or = 0.04). Subconjunctival IFN-gamma had no effect on the expression of ICAM-1 in the cornea.
authors.affiliation: Department of Ophthalmology, Loyola University Chicago, Maywood, IL 60153, USA.
authors.forename: C S
authors.initials: CS
authors.lastname: Bouchard
authors.forename: J B
authors.initials: JB
authors.lastname: Lasky
authors.forename: J E
authors.initials: JE
authors.lastname: Cundiff
authors.forename: B S
authors.initials: BS
authors.lastname: Smith
mesh_terms: D000818:Animals; D003228:Conjunctiva / Q000378:metabolism; D003315:Cornea / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005123:Eye / Q000378:metabolism*; D005260:Female; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism*; D007371:Interferon-gamma / Q000008:administration & dosage / Q000494:pharmacology*; D051381:Rats; D011917:Rats, Inbred Lew; D013194:Staining and Labeling; D013499:Surface Properties; D014018:Tissue Distribution; D015854:Up-Regulation
chemical_list: D018799:Intercellular Adhesion Molecule-1; D007371:Interferon-gamma