title: Calcium transported to isolated rat liver nuclei by nicotinamide adenine dinucleotide is insensitive to thapsigargin.
abstract: Calcium uptake by isolated nuclei was mediated by nicotinamide adenine dinucleotide. Oxidized nicotinamide nucleotide analogues were more effective mediators of nuclear calcium uptake. Thapsigargin inhibited ATP-mediated nuclear calcium transport without affecting NAD-mediated nuclear calcium uptake. Whilst DBHQ did not influence ATP-induced calcium transport, it did stimulate NAD-mediated nuclear calcium entry. Calcium channel blockers did not influence the action of NAD. This study provides a further mechanism for nuclear calcium transport regulated by changes in the cytosolic NAD(+)/NADH ratio.
authors.affiliation: Laboratoire de Neurobiologie Moléculaire des Interactions Cellulaires, Centre de Neurochimie du Centre National de la Recerche Scientifique, Strasbourg, France.
authors.forename: N
authors.initials: N
authors.lastname: Matter
authors.forename: A N
authors.initials: AN
authors.lastname: Malviya
mesh_terms: D000818:Animals; D001692:Biological Transport; D002118:Calcium / Q000378:metabolism*; D002132:Calcium Radioisotopes; D002467:Cell Nucleus / Q000378:metabolism; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D009243:NAD / Q000378:metabolism*; D051381:Rats; D013729:Terpenes / Q000494:pharmacology*; D019284:Thapsigargin
chemical_list: D002132:Calcium Radioisotopes; D013729:Terpenes; D009243:NAD; D019284:Thapsigargin; D002118:Calcium