Classification of sugar chains of glycoproteins by analyzing reducing end oligosaccharides obtained by partial acid hydrolysis.
Sugar chain types were classified on the basis of reducing end di- and trisaccharide structures. Sugar chains liberated from glycoproteins by the hydrazinolysis-N-acetylation method were pyridylaminated, and pyridylamino (PA-) sugar chains were purified by HPLC. The PA-sugar chains thus purified were partially hydrolyzed with 1 M trifluoroacetic acid. The acid hydrolysis conditions were investigated with the object of obtaining PA-di- and trisaccharides with high yields for different types of PA-sugar chains. The acid hydrolysates were separated by size-fractionation HPLC into PA-mono-, PA-di-, and PA-trisaccharides, and each fraction was analyzed by reversed-phase HPLC. The structures were then identified by comparing the HPLC elution positions with those of authentic PA-oligosaccharides derived from N-linked sugar chains and 12 types of O-linked sugar chains.
Makino
Y
Y
Department of Chemistry, Osaka University College of Science, Machikaneyama-cho 1-1, Osaka, Toyonaka, 560, Japan.
Kuraya
N
N
Omichi
K
K
Hase
S
S
D000107:Acetylation; D000143:Acids; D000818:Animals; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000032:analysis*; D002813:Chondrosarcoma / Q000737:chemistry; D004187:Disaccharides / Q000737:chemistry*; D006023:Glycoproteins / Q000737:chemistry*; D006834:Hydrazines / Q000737:chemistry; D006868:Hydrolysis; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D011509:Proteoglycans / Q000737:chemistry; D051381:Rats; D014312:Trisaccharides / Q000737:chemistry*
D000143:Acids; D002241:Carbohydrates; D004187:Disaccharides; D006023:Glycoproteins; D006834:Hydrazines; D011509:Proteoglycans; D014312:Trisaccharides