Inhibition of mucosal lipid hyperoxidation by green tea extract in 1,2-dimethylhydrazine-induced rat colonic carcinogenesis.
Phosphatidylcholine hydroperoxide (PCOOH) measured using a chemiluminescence detector to examine colonic mucosal lipid hyperoxidation increased after injection of 1,2-dimethylhydrazine and green tea extract (GTE), which we previously showed inhibited carcinogenesis and oxidative DNA damage in the gastrointestinal tract. Therefore, the hyperoxidation of membrane phospholipids reflected well the degree of DNA damage and carcinogenic alteration, and may be a useful intermediate biomarker for initiation of carcinogenesis.
Matsumoto
H
H
First Department of Surgery, Kyoto Prefectural University of Medicine, Japan.
Yamane
T
T
Inagake
M
M
Nakatani
H
H
Iwata
Y
Y
Takahashi
T
T
Nishimura
H
H
Nishino
H
H
Nakagawa
K
K
Miyazawa
T
T
D019813:1,2-Dimethylhydrazine; D000080242:8-Hydroxy-2'-Deoxyguanosine; D000818:Animals; D003110:Colonic Neoplasms / Q000139:chemically induced / Q000378:metabolism*; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000032:analysis; D004127:Dimethylhydrazines / Q000633:toxicity; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D010713:Phosphatidylcholines / Q000032:analysis; D010936:Plant Extracts / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013662:Tea
D004127:Dimethylhydrazines; D010713:Phosphatidylcholines; D010936:Plant Extracts; D013662:Tea; C057590:phosphatidylcholine hydroperoxide; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine; D019813:1,2-Dimethylhydrazine