Monoclonal antibody against the fusion junction of a deletion-mutant epidermal growth factor receptor.
A mouse monoclonal antibody (IgG2b), 3C10, was produced against the truncated epidermal growth factor receptor (EGFR), encoded by the (type III) in-frame deletion mutation of 801 nucleotides of EGFR affecting the external domain, known to be expressed in some human glioblastoma. As this mutation newly generates a glycine residue at the fusion point, a 14 amino acid peptide around the fusion junction including this glycine was chemically synthesised and used for immunisation of (B6 x DBA/2) F1 mice. Flow cytometric analysis showed 3C10 antibody staining of a mouse NIH/3T3 transfectant (ERM5) with the type III EGFR deletion-mutant gene, but not one with wild-type EGFR. The antibody immunoprecipitated the truncated EGFR protein with a molecular mass of approximately 140 kDa from ERM5 cells. Immunostaining of glioblastomas revealed binding in the case with the type III EGFR mutation, the five other specimens without the mutation being negative despite overexpression of EGFR in some cases.
Okamoto
S
S
Laboratory of Immunology, Aichi Cancer Center Research Institute, Nagoya, Japan.
Yoshikawa
K
K
Obata
Y
Y
Shibuya
M
M
Aoki
S
S
Yoshida
J
J
Takahashi
T
T
D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D001483:Base Sequence; D001932:Brain Neoplasms / Q000235:genetics* / Q000473:pathology; D017931:DNA Primers; D015342:DNA Probes; D066246:ErbB Receptors / Q000032:analysis / Q000235:genetics* / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D005909:Glioblastoma / Q000235:genetics* / Q000473:pathology; D005910:Glioma; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000276:immunology; D017384:Sequence Deletion; D014162:Transfection; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D017931:DNA Primers; D015342:DNA Probes; D009838:Oligodeoxyribonucleotides; D010455:Peptides; D011994:Recombinant Proteins; D066246:ErbB Receptors