Analysis of linear oligogalacturonic acids by negative-ion electrospray ionization mass spectrometry.
Linear oligogalacturonic acids (1,4-linked alpha-D-galacturonic acid oligomers), obtained by partial acid hydrolysis of orange polygalacturonides, were studied by negative-ion electrospray ionization mass spectrometry, without prior sample derivatization. After preparative separation using high-resolution anion-exchange chromatography, some fractions enriched in uronic acids were desalted, transferred into a methanol+water solution adjusted to pH 10, and directly submitted to electrospray ionization mass spectrometry in the negative-ion recording mode. Clear molecular mass assignments of the oligomers, covering a degree of polymerization between 4 and 7, were obtained.
Xie
M
M
Institut National Agronomique, Laboratoire de Chimie Analytique, Parix, France.
Giraud
D
D
Bertheau
Y
Y
Casetta
B
B
Arpino
P
P
D002240:Carbohydrate Sequence; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000032:analysis*; D010368:Pectins / Q000032:analysis*
D009844:Oligosaccharides; D010368:Pectins; C003181:polygalacturonic acid