In vivo and in vitro toxicity of newly synthesized monofunctional sulfur mustard derivatives.
The toxicity of two new monofunctional sulfur mustard derivatives was tested. The compound (4-carboxybutyl 2-chloroethyl sulfide, CBCS; 10-carboxydecyl 2-chloroethyl sulfide, CDCS) possess the 2-chloroethyl sulfide moiety present in mustard gas. Exposure of guinea pig skin to CBCS resulted in a dose-related ulcerative effect. CDCS exhibited similar pathological effects. Dimethylsulfoxide (DMSO) exacerbated CBCS toxicity. Regeneration and healing were prominent six days after application. Concentration-related effects were found in in vitro systems, using human SH-SY5Y neuroblastoma cells for acute toxicity and Y79 retinoblastoma cells for colony forming assay. CBCS or derivatives may serve as models compounds for investigating the mechanism of action of alkylating agents.
Wormser
U
U
Department of Pharmacology, Faculty of Medicine, Jerusalem, Israel.
Green
B S
BS
Arad-Yellin
R
R
Brodsky
B
B
Shatz
I
I
Nyska
A
A
D000287:Administration, Topical; D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D006168:Guinea Pigs; D006801:Humans; D009150:Mustard Compounds / Q000633:toxicity*; D009447:Neuroblastoma; D012175:Retinoblastoma; D012867:Skin / Q000187:drug effects; D014407:Tumor Cells, Cultured
C099676:10-carboxydecyl-2-chloroethylsulfide; C099675:4-carboxybutyl-2-chloroethylsulfide; D009150:Mustard Compounds