Efficient transfer of regulated genes in adipocytes and hepatoma cells by the combination of liposomes and replication-deficient adenovirus.
Efficient transfer of genes maintaining a correct hormonal control in transfected cells is the prerequisite for gene regulation studies and for gene therapy. Differentiated cells, like adipocytes or hepatocytes, are difficult to transfect. In an attempt to improve gene transfer, we first transiently transfected cultured 3T3-F442A adipocytes with a construct containing the simian virus 40 (SV40) promoter fused to the chloramphenicol acetyltransferase (CAT) gene (pSV2-CAT), using various cationic liposomes. Among these, only lipofectAMINE was five times more efficient than the standard calcium phosphate procedure. To further augment efficiency, we transfected 3T3-F442A adipocytes and FAO hepatoma cells with the lipofectAMINE/pSV2-CAT complex in the presence of replication-deficient recombinant type-5 adenovirus at 200 pfu/cell. CAT activity of transiently transfected cells was increased about 50-fold when compared to the calcium phosphate procedure. To determine whether this methodology would be useful for obtaining stable transfectants and would not interfere with correct gene regulation, we used a construct containing -2100 to +69 bp of the phosphoenolpyruvate carboxykinase gene fused to the CAT gene (pPL1-CAT). This construct was shown previously to be cAMP-responsive after calcium-phosphate-mediated transfection of adipocytes and hepatoma cells. 3T3-F442A or FAO cells in which pPL1-CAT was either transiently or stably transferred by lipofectAMINE and adenovirus responded to isoproterenol or cAMP, respectively, with a 2-3-fold increase in CAT activity. Therefore the association of liposomes and adenovirus is an efficient method for transient or stable transfer of regulated genes in adipocytes and hepatoma cells.
Meunier-Durmort
C
C
Centre de Recherche sur l'Endocrinologie Moléculaire et le Développement, CNRS, Meudon, France.
Ferry
N
N
Hainque
B
B
Delattre
J
J
Forest
C
C
D016475:3T3 Cells; D000260:Adenoviruses, Human / Q000235:genetics* / Q000502:physiology; D000818:Animals; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D005786:Gene Expression Regulation; D018014:Gene Transfer Techniques; D008055:Lipids / Q000737:chemistry; D008081:Liposomes / Q000737:chemistry; D008114:Liver Neoplasms, Experimental / Q000235:genetics; D051379:Mice; D051381:Rats; D013997:Time Factors; D014162:Transfection; D014407:Tumor Cells, Cultured; D014779:Virus Replication
D008055:Lipids; D008081:Liposomes; D015500:Chloramphenicol O-Acetyltransferase