A candidate gene for RNA export carrier protein has two RBDs (RNA binding domain and Ran binding domain).
The human Ras-related nuclear protein Ran/TC4 is the member of a well conserved family of GTPases that can regulate cell-cycle progression, nuclear structure, protein import, and RNA export through nuclear pore complex (NPC). Translocation of RNA through the NPC also needs a RNA-binding protein as a possible mediator of export. By a low-stringency hybridization with a PCR fragment encoding RNA-binding domain as a probe, we obtained a partial human cDNA from skeletal muscle cDNA library. The deduced amino acid sequence indicated that the gene possessed both RNA-binding domain and Ran-binding domain, therefore it is a candidate gene for RNA export carrier protein. RNA blot analysis showed that this gene appeared approximately 8-10 kilo base in length and that the gene expression level in several human tissues is ubiquitous. The function of the gene will be discussed.
Endo
H
H
Department of Biochemistry, Jichi Medical School, Tochigi, Japan.
Ueno
E
E
Ichida
M
M
Tsukamoto
T
T
Hamamoto
T
T
Kagawa
Y
Y
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D005796:Genes; D006801:Humans; D066298:In Vitro Techniques; D051379:Mice; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D020931:ran GTP-Binding Protein
D017931:DNA Primers; D018076:DNA, Complementary; D009687:Nuclear Proteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D020931:ran GTP-Binding Protein