Identification of two prolactin cDNA sequences from a goldfish pituitary cDNA library.
We have identified two different cDNA clones encoding for goldfish prolactin (gfPRL) from a pituitary cDNA library. The coding regions of these clones are predicted to encode for an identical amino acid (aa) sequence with four silent mutations. The 3'-untranslated regions (UTRs) of these clones show only 72% nucleotide (nt) sequence identity. The two genes each encoding gfPRL might have derived from recent gene duplication before the divergence of goldfish from other Cypriniforms. Genomic Southern blot analysis of goldfish DNA also demonstrated that there is a small family of two genes for prolactin in the genome of goldfish.
Chan
Y H
YH
Department of Biochemistry, Chinese University of Hong Kong, Shatin, N.T., Hong Kong.
Cheng
K W
KW
Yu
K L
KL
Chan
K M
KM
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D006054:Goldfish / Q000235:genetics*; D008969:Molecular Sequence Data; D010902:Pituitary Gland / Q000737:chemistry*; D011388:Prolactin / Q000737:chemistry / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D011388:Prolactin