Oxytocin affects the release of steroids, insulin-like growth factor-I, prostaglandin F2alpha and cyclic nucleotides by human granulosa cells in vitro.
The aim of the present experiments was to examine the effects of oxytocin (1-10 000 ng/ml) on hormone and cyclic nucleotide secretion by human granulosa cells cultured in a serum-supplemented medium. The release of progesterone, oestradiol, insulin-like growth factor-I, prostaglandin F2alpha, cAMP and cGMP into the incubation medium was analysed by radioimmunoassay. An inhibition of progesterone, but not of oestradiol release was observed. Oxytocin also stimulated insulin-like growth factor-I, prostaglandin F2alpha, cAMP and cGMP output. The results suggest an involvement of oxytocin in the autocrine/paracrine regulation of steroid, insulin-like growth factor, prostaglandin and cyclic nucleotide release by human ovarian cells.
Sirotkin
A V
AV
Research Institute of Animal Production, 949 92 Nitra, Slovakia.
Schaeffer
H J
HJ
Mlyncek
M
M
Missik
J
J
Bulla
J
J
D000328:Adult; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000378:metabolism; D006152:Cyclic GMP / Q000378:metabolism; D015237:Dinoprost / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004958:Estradiol / Q000378:metabolism; D005260:Female; D006107:Granulosa Cells / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000378:metabolism*; D009712:Nucleotides, Cyclic / Q000378:metabolism*; D010121:Oxytocin / Q000008:administration & dosage / Q000494:pharmacology*; D011374:Progesterone / Q000378:metabolism; D013256:Steroids / Q000378:metabolism*
D009712:Nucleotides, Cyclic; D013256:Steroids; D011374:Progesterone; D004958:Estradiol; D010121:Oxytocin; D007334:Insulin-Like Growth Factor I; D015237:Dinoprost; D000242:Cyclic AMP; D006152:Cyclic GMP