title: 5-Nitrosothio-2-nitrobenzoate: a reagent for the nitrosation of thiol groups in proteins.
abstract: The S-nitroso derivative of 5-thio-2-nitrobenzoate was synthesized from 5,5'-dithiobis(2-nitrobenzoic acid) and partially characterized. Although relatively unstable, it is easy to prepare and reacts very rapidly with thiols and thiol groups of proteins to give corresponding S-nitrosothiols and 5-thio-2-nitrobenzoate dianion. The latter's easy spectrophotometric detection makes such reactions easy to follow and to quantitate.
authors.affiliation: Department of Biochemistry, The Ohio State University, 484 West 12th Avenue, Columbus, Ohio, 43210, USA.
authors.forename: M S
authors.initials: MS
authors.lastname: Studebaker
authors.forename: H
authors.initials: H
authors.lastname: Zhang
authors.forename: G E
authors.initials: GE
authors.lastname: Means
mesh_terms: D000818:Animals; D002417:Cattle; D007202:Indicators and Reagents / Q000138:chemical synthesis; D009603:Nitroso Compounds / Q000138:chemical synthesis; D011506:Proteins / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry; D013053:Spectrophotometry; D013438:Sulfhydryl Compounds / Q000737:chemistry*
chemical_list: C101007:5-nitrosothio-2-nitrobenzoate; D007202:Indicators and Reagents; D009603:Nitroso Compounds; D011506:Proteins; D013438:Sulfhydryl Compounds; D012710:Serum Albumin, Bovine