Hel-N1, an RNA-binding protein, is a ligand for an A + U rich region of the GLUT1 3' UTR.
Hel-N1, is an RRM protein which is a mammalian homologue of the Drosophila melanogaster RNA binding protein, ELAV (embryonic lethal abnormal vision). Hel-N1 binds to RNA containing short stretches of uridylates similar to those found in the 3' untranslated regions (3'-UTRs) of oncoprotein and cytokine mRNAs. The GLUT1 glucose transporter has an extensive 3' UTR that is AU-rich reminiscent of the 3'UTR of an oncogene mRNA. An in vitro RNA binding assay using Hel-N1 demonstrated binding to a specific portion of the GLUT1 3'UTR. Analysis of the folding pattern of this region depicted the retention of a stem loop structure, wherein the loop is composed of a stretch of uridylates. To further analyze the potential function of Hel-N1, stable transfectants were made in the 3T3-L1 cell line. The transfectants have been characterized, and the presence of the Hel-N1 DNA and protein verified. Data indicate Hel-N1 is a ligand for GLUT1 and its binding affects the stability and translatability of the GLUT1 message.
Jain
R G
RG
School of Medicine, East Carolina University, Greenville, NC 27834, USA.
Andrews
L G
LG
McGowan
K M
KM
Gao
F
F
Keene
J D
JD
Pekala
P P
PP
D016475:3T3 Cells; D000818:Animals; D001482:Base Composition; D001483:Base Sequence; D004331:Drosophila melanogaster; D051959:ELAV Proteins; D000067777:ELAV-Like Protein 2; D051272:Glucose Transporter Type 1; D006801:Humans; D008024:Ligands; D051379:Mice; D008969:Molecular Sequence Data; D009004:Monosaccharide Transport Proteins / Q000235:genetics*; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009690:Nucleic Acid Conformation; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000378:metabolism*; D012689:Sequence Homology, Nucleic Acid
D051959:ELAV Proteins; D000067777:ELAV-Like Protein 2; C086811:ELAVL2 protein, human; D051272:Glucose Transporter Type 1; D008024:Ligands; D009004:Monosaccharide Transport Proteins; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; C495677:SLC2A1 protein, human; C495678:Slc2a1 protein, mouse