title: Novel specific endonuclease activity recognizing a 10-bp sequence.
abstract: We report here the generation of a novel restriction endonuclease (ENase) activity with the 10-bp recognition sequence, [symbol: see text]. This specificity could be achieved by first methylating a substrate DNA with M.MamI in vivo, followed by in vitro R.DpnI restriction.
authors.affiliation: Department of Genetics, Yale University School of Medicine, New Haven, CT 06510, USA. LEROTC@biomed.med.yale.edu
authors.forename: H M
authors.initials: HM
authors.lastname: Striebel
authors.forename: C
authors.initials: C
authors.lastname: Kessler
mesh_terms: D001482:Base Composition; D001483:Base Sequence; D004247:DNA / Q000378:metabolism; D004262:DNA Restriction Enzymes / Q000378:metabolism*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism*; D008969:Molecular Sequence Data; D013379:Substrate Specificity
chemical_list: D004247:DNA; D004262:DNA Restriction Enzymes; C064059:endodeoxyribonuclease BsaAI; C020102:endodeoxyribonuclease DpnI; D015252:Deoxyribonucleases, Type II Site-Specific