title: Determination of alternariol and alternariol methyl ether in apple juice using solid-phase extraction and high performance liquid chromatography.
abstract: The present work describes a new method for determination of alternariol (AOH) and alternariol methyl ether (AME) in apple juice using solid-phase extraction (SPE) columns for extraction and cleanup of samples for high-performance liquid chromatography (HPLC). Chromatograms of spiked samples show that both toxins can be easily detected without interferences, and good recoveries for AOH (82.8 +/- 7.4%) and AME (91.9 +/- 6.1%) with detection limits as low as 1.6 and 0.7 micrograms/l, respectively, were obtained.
authors.affiliation: Instituto Fermentaciones Industriales, Consejo Superior de Investigaciones Científicas (C.S.I.C.), Madrid, Spain.
authors.forename: T
authors.initials: T
authors.lastname: Delgado
authors.forename: C
authors.initials: C
authors.lastname: Gómez-Cordovés
authors.forename: P M
authors.initials: PM
authors.lastname: Scott
mesh_terms: D001628:Beverages / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005638:Fruit; D007783:Lactones / Q000032:analysis*; D009183:Mycotoxins / Q000032:analysis*; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
chemical_list: D007783:Lactones; D009183:Mycotoxins; C005197:alternariol; C018206:alternariol monomethyl ether