Cloning, sequencing and functional studies of the gene encoding human GTP cyclohydrolase I.
We have identified a genomic clone containing the 5' regulatory region of the gene GTP-CH encoding human GTP cyclohydrolase I. The transcription start point (tsp) was mapped by 5'-rapid amplification of cDNA ends (5'-RACE). The 2.6-kb region upstream from the tsp showed promoter activity when ligated upstream from a reporter gene. The truncation of approximately 2 kb of the promoter did not change expression activity, while a further removal of 243 bp halved the activity. The promoter contains CCAAT and TATA boxes. The GC-rich region close to the tsp, which contains several putative Sp1-responsive elements, is required for maximum promoter activity. Interferon-gamma treatment of B-cells transfected with reporter constructs had no influence on the expression activity.
Witter
K
K
GSF-Institut für Klinische Molekularbiologie, München, Germany.
Werner
T
T
Blusch
J H
JH
Schneider
E M
EM
Riess
O
O
Ziegler
I
I
Rödl
W
W
Bacher
A
A
Gütlich
M
M
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002460:Cell Line / Q000187:drug effects; D003001:Cloning, Molecular; D004331:Drosophila melanogaster / Q000201:enzymology / Q000235:genetics; D006136:GTP Cyclohydrolase / Q000235:genetics* / Q000378:metabolism; D017930:Genes, Reporter; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology; D008969:Molecular Sequence Data; D012045:Regulatory Sequences, Nucleic Acid; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D014162:Transfection
D007371:Interferon-gamma; D006136:GTP Cyclohydrolase