Possible involvement of C5/C5a in the efferent and elicitation phases of contact sensitivity.
The elicitation of 24-h contact sensitivity (CS) in mice requires a serotonin-dependent, 2-h response called CS initiation. We studied the role of complement (C) by comparing CS in DBA/1 (C5-normal) vs DBA/2 (C5-deficient) mice and found impaired 2-h, but not 24-h, CS. We showed previously that 2-h responses represent CS initiation and are required for elicitation of 24-h responses. Treatment of C5-deficient mice with absent macroscopic responses (ear swelling) with a selective serotonin antagonist inhibited 24-h CS, suggesting that C5-deficient mice had submacroscopic (i.e., microscopic), serotonin-dependent CS initiation. When normal mouse serum was used as a source of C5 to reconstitute C5-deficient mice, significant 2-h responses were restored. Furthermore, heat treatment of normal mouse serum to inactivate C abrogated restoration of 2-h responses. Thus, C5 was suggested to be involved in CS initiation. Using a suboptimal immunizing dose of Ag revealed an impaired 24-h component of CS in DBA/2 mice, but not in DBA/1 mice, and also in C5-deficient B10.D2/o mice compared with C5-normal B10.D2/n mice with a suboptimal eliciting dose of Ag. Again, reconstitution of B10.D2/o mice with normal mouse serum restored deficient 24-h CS responses. Thus, 2-h and classical 24-h CS probably depend in part on C5. These results imply that C5 may play a role in the elicitation of 24-h CS, probably via required preceding CS initiation.
Tsuji
R F
RF
Noda Institute for Scientific Research, KIKKOMAN Corporation, Japan.
Kikuchi
M
M
Askenase
P W
PW
D000818:Animals; D000941:Antigens / Q000008:administration & dosage; D003182:Complement C5 / Q000502:physiology*; D015936:Complement C5a / Q000502:physiology*; D003877:Dermatitis, Contact / Q000276:immunology*; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D007650:Ketanserin / Q000494:pharmacology; D051379:Mice; D008815:Mice, Inbred Strains; D011985:Receptors, Serotonin / Q000502:physiology; D012702:Serotonin Antagonists / Q000494:pharmacology
D000941:Antigens; D003182:Complement C5; D011985:Receptors, Serotonin; D012702:Serotonin Antagonists; D015936:Complement C5a; D007650:Ketanserin