The reducing ability of iron chelates by NADH-cytochrome B5 reductase or cytochrome B5 responsible for NADH-supported lipid peroxidation.
We studied iron reduction, NADH oxidation, and lipid peroxidation in the presence of iron chelates with a chelator, such as nitrilotriacetate, ADP, citrate, and pyrophosphate, in NADH-supported reconstituted system. The results showed the selectivity of NADH-cytochrome b5 reductase or cytochrome b5 towards iron chelates and the subsequent ability of this system to promote peroxidation. The lipid peroxidation was partially inhibited by superoxide dismutase. In the presence of any iron chelate, hydrogen peroxide was produced in the systems including the reductase, and the production was accompanied with an increase in NADH oxidation.
Miura
A
A
Division of Environmental Hygiene, Hokkaido College of Pharmacy, Japan.
Tampo
Y
Y
Yonaha
M
M
D000244:Adenosine Diphosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002951:Citrates / Q000378:metabolism; D019343:Citric Acid; D003579:Cytochrome Reductases / Q000378:metabolism*; D042966:Cytochrome-B(5) Reductase; D015786:Cytochromes b5 / Q000378:metabolism*; D011756:Diphosphates / Q000378:metabolism; D007502:Iron Chelating Agents / Q000378:metabolism*; D015227:Lipid Peroxidation / Q000187:drug effects; D008862:Microsomes, Liver / Q000378:metabolism; D008954:Models, Biological; D009243:NAD / Q000378:metabolism*; D009249:NADP / Q000378:metabolism; D010084:Oxidation-Reduction; D051381:Rats
D002951:Citrates; D011756:Diphosphates; D007502:Iron Chelating Agents; D009243:NAD; D019343:Citric Acid; D009249:NADP; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D015786:Cytochromes b5; D003579:Cytochrome Reductases; D042966:Cytochrome-B(5) Reductase