Differential induction of 3-hydroxy-3-methylglutaryl CoA reductase in two cotton species following inoculation with Verticillium.
Gossypium barbadense cottons are typically more resistant to wilt pathogens than are Gossypium hirsutum cultivars. Both species make terpenoid phytoalexins in response to infection, implicating isoprenoid biosynthesis as a factor in resistance. Conserved regions in plant 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR), the first enzyme in the terpene biosynthesis pathway, were used to design polymerase chain reaction primers for cloning a fragment of a cotton HMGR gene. The clone was used as a probe on Northern blots to show that induction of HMGR mRNA following introduction of Verticillium dahliae spores into the vascular system is much more rapid in Seabrook Sea Island, a restant G. barbadense cotton, than it is in Rowden, a susceptible G. hirsutum. The amount of HMGR mRNA returned to near control levels in 4 days in the former variety but continued to accumulate in the latter. Specific enzyme activity of HMGR also increased more rapidly in stele extracts of Seabrook Sea Island than in Rowden.
Joost
O
O
Department of Plant Pathology and Microbiology, Texas A&M University College Station 77843, USA.
Bianchini
G
G
Bell
A A
AA
Benedict
C R
CR
Magill
C W
CW
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004790:Enzyme Induction; D003368:Gossypium / Q000201:enzymology / Q000382:microbiology*; D006903:Hydroxymethylglutaryl CoA Reductases / Q000096:biosynthesis* / Q000235:genetics; D007113:Immunity, Innate; D003904:Mitosporic Fungi / Q000472:pathogenicity*; D015336:Molecular Probe Techniques; D008969:Molecular Sequence Data; D010935:Plant Diseases; D012333:RNA, Messenger / Q000096:biosynthesis; D018749:RNA, Plant / Q000096:biosynthesis; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D013729:Terpenes / Q000378:metabolism
D012333:RNA, Messenger; D018749:RNA, Plant; D013729:Terpenes; D006903:Hydroxymethylglutaryl CoA Reductases