The homeotic target gene centrosomin encodes an essential centrosomal component.
The centrosomin (cnn) gene encodes a protein associated with mitotic centrosomes in Drosophila melanogaster and is a target of homeotic gene regulation. Here we report that CNN is an essential component of the centrosome. Loss of zygotic cnn expression disrupts the development of the second midgut constriction, the gastric caeca, and the nervous system. Embryos that lack maternal as well as zygotic cnn expression display defects in nuclear division, chromosome alignment, and microtubule organization, while adult flies that are mosaic for cnn-cells exhibit defects indicative of a block in cell proliferation. We propose that cnn provides an example of homeotic genes directly regulating the accumulation of essential cellular proteins to carry out segment-specific morphogenetic functions.
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Howard Hughes Medical Institute Department of Biology Indiana University, Bloomington 47405, USA.
Kaufman
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D000818:Animals; D001756:Blastoderm / Q000502:physiology; D002453:Cell Cycle / Q000502:physiology; D002467:Cell Nucleus / Q000235:genetics; D018385:Centrosome / Q000737:chemistry / Q000502:physiology*; D002999:Clone Cells / Q000502:physiology; D003593:Cytoplasm / Q000737:chemistry; D004330:Drosophila / Q000196:embryology / Q000235:genetics*; D029721:Drosophila Proteins; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000502:physiology; D018095:Germ-Line Mutation / Q000502:physiology; D018398:Homeodomain Proteins / Q000235:genetics* / Q000378:metabolism; D016350:Leucine Zippers / Q000235:genetics; D008297:Male; D008870:Microtubules / Q000737:chemistry; D009024:Morphogenesis / Q000235:genetics; D016296:Mutagenesis / Q000502:physiology; D009866:Oogenesis / Q000502:physiology; D010766:Phosphorylation; D013091:Spermatogenesis / Q000502:physiology
D029721:Drosophila Proteins; D018398:Homeodomain Proteins; C098130:cnn protein, Drosophila