Expression of inositol 1,4,5-trisphosphate receptors in rat adrenocortical zones.
Previously we demonstrated the presence of InsP3R-I, -II and -III subtypes in the zona glomerulosa. Now we have examined the expression of different subtypes of inositol 1,4,5-trisphosphate receptor (InsP3R) in the inner zones of rat adrenal cortex. RNA extracted from decapsulated adrenal tissue (zonae fasciculata-reticularis and the medulla) or from fasciculata-reticularis cells was reverse transcribed. Subsequent polymerase chain reaction revealed the presence of InsP3R-I, -II and -III subtypes in decapsulated tissue but failed to demonstrate the expression of any known subtypes of InsP3R in fasciculata-reticularis cells. Accordingly, InsP3 receptors expressed in the decapsulated tissue are of medullary origin.
Szabadkai
G
G
Department of Physiology, Semmelweis University of Medicine, Budapest, Hungary.
Horváth
A
A
Rohács
T
T
Vimláti
L
L
Spät
A
A
Enyedi
P
P
D000199:Actins / Q000737:chemistry / Q000235:genetics; D000302:Adrenal Cortex / Q000378:metabolism; D000818:Animals; D001483:Base Sequence; D015220:Calcium Channels / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D053496:Inositol 1,4,5-Trisphosphate Receptors; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D012326:RNA Splicing; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D018160:Receptors, Cytoplasmic and Nuclear / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D015383:Zona Fasciculata / Q000737:chemistry / Q000166:cytology / Q000378:metabolism*; D015385:Zona Reticularis / Q000737:chemistry / Q000166:cytology / Q000378:metabolism*
D000199:Actins; D015220:Calcium Channels; D018076:DNA, Complementary; D053496:Inositol 1,4,5-Trisphosphate Receptors; D012333:RNA, Messenger; D018160:Receptors, Cytoplasmic and Nuclear