Cloning of a DNA repeat element from horse: DNA sequence and chromosomal localization.
A DNA repeat element, revealed initially by digestion of horse DNA with TaqI, was cloned and characterized by Southern and in situ hybridization studies and nucleotide sequencing. The clone, e4/1, consisted of 32 tandem reiteration of a unit repeat of 21-22 bp, and produced multilocus DNA fingerprinting profiles that were useful for parentage analysis in horses. The tandem repeat element was shown by in situ hybridization to be localized in the centromeres of the acrocentric but not metacentric classes of horse chromosomes.
Broad
T E
TE
AgResearch, Grasslands Research Centre, Palmerston North, New Zealand.
Forrest
J W
JW
Lewis
P E
PE
Pearce
P D
PD
Phua
S H
SH
Pugh
P A
PA
Stewart-Scott
I A
IA
D000818:Animals; D001483:Base Sequence; D002503:Centromere; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D016384:Consensus Sequence / Q000235:genetics; D016172:DNA Fingerprinting; D005220:Fathers; D005260:Female; D006736:Horses / Q000235:genetics*; D017403:In Situ Hybridization; D008297:Male; D008969:Molecular Sequence Data; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid