title: The sensitivity of in vitro erythropoietic progenitor cells to different erythropoietin reagents during development and the role of cell death in culture.
abstract: With the availability of several recombinant erythropoietin (Epo) reagents, it has been possible to undertake a systematic study of the relative Epo sensitivity of late erythroid colony-forming units (CFU-E) in 8.5-day embryos, 13.5-day fetal liver, and adult bone marrow of the mouse. All Epo preparations tested, including one from impure sheep and a highly purified human native Epo preparation, produced parallel, but displaced, dose-response curves when Epo concentration was plotted against percent CFU-E response calculated from the optimal Epo concentration. It was found that the CFU-E derived from 8.5-day embryos demonstrated the greatest Epo sensitivity which decreased in fetal liver and adult bone marrow CFU-E populations. Modifications to the culture system allowed CFU-E to be stimulated with as little as 0.003 mU/mL, equivalent to approximately 0.03 fg Epo. Under these culture conditions, no evidence for apoptosis was found, although a normal programmed cell death function cannot be ruled out.
authors.affiliation: Department of Transfusion Medicine, University of U1m, Germany.
authors.forename: F
authors.initials: F
authors.lastname: Zimmermann
authors.forename: I N
authors.initials: IN
authors.lastname: Rich
mesh_terms: D000818:Animals; D017209:Apoptosis; D001853:Bone Marrow / Q000187:drug effects; D016923:Cell Death; D002478:Cells, Cultured; D004249:DNA Damage; D004305:Dose-Response Relationship, Drug; D004351:Drug Resistance; D004622:Embryo, Mammalian / Q000187:drug effects / Q000502:physiology; D015672:Erythroid Precursor Cells / Q000187:drug effects*; D004920:Erythropoiesis / Q000187:drug effects* / Q000502:physiology; D004921:Erythropoietin / Q000494:pharmacology*; D005260:Female; D008099:Liver / Q000187:drug effects / Q000196:embryology; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D011994:Recombinant Proteins / Q000494:pharmacology*
chemical_list: D011994:Recombinant Proteins; D004921:Erythropoietin