A role for the KP leucine zipper in regulating P element transposition in Drosophila melanogaster.
The KP element can repress P element mobility in Drosophila melanogaster. Three mutant KP elements were made that had either two amino acid substitutions or a single amino acid deletion in the putative leucine zipper domain found in the KP polypeptide. Each KP element was expressed from the actin 5C proximal promoter. The wild-type control construct strongly repressed P element mobility, measured by the GD sterility and sn(w) mutability assays, in a position-independent manner. The single amino acid deletion mutant failed to repress P mobility by the double amino acid substitution mutants was position dependent. The results show that the leucine zipper of the KP polypeptide is important for P element regulation. This supports the multimer-poisoning model of P element repression, because leucine zipper motifs are involved in protein-protein interactions.
Andrews
J D
JD
Department of Biochemistry, University of Western Ontario, London, Canada.
Gloor
G B
GB
D000199:Actins / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D004251:DNA Transposable Elements; D004331:Drosophila melanogaster / Q000235:genetics*; D008040:Genetic Linkage; D005838:Genotype; D016350:Leucine Zippers; D008540:Meiosis; D008969:Molecular Sequence Data; D016296:Mutagenesis; D016297:Mutagenesis, Site-Directed; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D011995:Recombination, Genetic; D012097:Repressor Proteins / Q000096:biosynthesis / Q000235:genetics; D015183:Restriction Mapping
D000199:Actins; D017931:DNA Primers; D004251:DNA Transposable Elements; D011993:Recombinant Fusion Proteins; D012097:Repressor Proteins