title: Oocyte donation programme: results obtained with intracytoplasmic sperm injection in cases of severe male factor infertility or previous failed fertilization.
abstract: Intracytoplasmic sperm injection was carried out in 15 oocyte donation cycles of 15 infertile couples where oocytes had failed to fertilize after in-vitro fertilization (IVF) procedures or where the male partner had severe male factor infertility. A total of 62 oocytes were donated, but only 46 of these, in metaphase II, were injected. Of the injected oocytes, 31 (67.3%) had two pronuclei the morning after the injection procedure. On the following day, 29 embryos were obtained (93% of the fertilized oocytes) and 25 were transferred. Two patients were not successful and consequently did not undergo embryo transfer. A total of five clinical pregnancies were obtained, giving pregnancy rates of 33.3 and 38.4% per started cycle and embryo transfer respectively.
authors.affiliation: TECNOBIOS, Centre for Reproductive Health, Bologna, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Borini
authors.forename: M G
authors.initials: MG
authors.lastname: Bafaro
authors.forename: L
authors.initials: L
authors.lastname: Bianchi
authors.forename: F
authors.initials: F
authors.lastname: Violini
authors.forename: M A
authors.initials: MA
authors.lastname: Bonu
authors.forename: C
authors.initials: C
authors.lastname: Flamigni
mesh_terms: D000328:Adult; D003593:Cytoplasm; D004624:Embryo Transfer; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D006801:Humans; D007246:Infertility / Q000628:therapy*; D007248:Infertility, Male / Q000628:therapy; D008297:Male; D008845:Microinjections; D018587:Oocyte Donation; D011247:Pregnancy; D011256:Pregnancy Outcome; D013094:Spermatozoa