Molecular cloning of thi-4, a gene necessary for the biosynthesis of thiamine in Neurospora crassa.
The thiamine-4 (thi-4) gene was cloned by functional complementation of a thi-4 mutant of Neurospora crassa. The product of this gene is believed to be involved in the condensation of pyrimidine and thiazole precursors, which is necessary for the synthesis of thiamine. The thi-4 gene has ten introns which vary in length from 57 to 200 bp and the junction and internal (lariat) sequences are in good agreement with the consensus sequences for the splicing of introns in N. crassa. The thi-4 gene encodes a protein of 538 amino acids which is similar in terms of amino-acid sequence to proteins encoded by Saccharomyces cerevisiae whose function is unknown. The expression of the thi-4 gene in N. crassa was not repressed by thiamine.
Akiyama
M
M
Department of Biology, Faculty of Science, Okayama University, Okayama 700, Japan.
Nakashima
H
H
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics*; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D007438:Introns; D008969:Molecular Sequence Data; D009492:Neurospora crassa / Q000235:genetics* / Q000378:metabolism*; D012150:Polymorphism, Restriction Fragment Length; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins; D017386:Sequence Homology, Amino Acid; D013831:Thiamine / Q000096:biosynthesis*
D017931:DNA Primers; D004271:DNA, Fungal; D005656:Fungal Proteins; D029701:Saccharomyces cerevisiae Proteins; C089358:THI4 protein, S cerevisiae; D013831:Thiamine