Label-free observation of DNA-hybridisation and endonuclease activity on a wave guide surface using a grating coupler.
Hybridisation of nucleic acid oligomers to an immobilised target has been observed in real time using evanescent field technology. A biotinylated 24-mer with random sequence including the EcoRI recognition site was immobilised via streptavidin onto a grating coupler wave guide surface. Hybridisation of 22-mer, 15-mer and 8-mer was observed. Activity of restriction endonuclease EcoRI was visualised by measurement of the loss of bound DNA after incubation.
Bier
F F
FF
Institute of Biochemistry and Molecular Physiology, University of Potsdam, Berlin, Germany.
Scheller
F W
FW
D001483:Base Sequence; D015374:Biosensing Techniques; D003199:Computer Systems; D004247:DNA / Q000032:analysis*; D015246:Deoxyribonuclease EcoRI / Q000032:analysis*; D007700:Kinetics; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D055095:Optics and Photonics; D013499:Surface Properties; D013698:Templates, Genetic; D014159:Transducers
D004247:DNA; D015246:Deoxyribonuclease EcoRI