title: Site-directed mutational analysis of the osmotically regulated proU promoter of Salmonella typhimurium.
abstract: We carried out PCR mutagenesis of the proU promoter of Salmonella typhimurium, in order to identify sequences important for its osmotic control. We obtained five mutations in the -35 element: two decreased the promoter strength, one increased it, and the others had no effect. However, none abolished osmotic control, suggesting that the sequence of the -35 element is not crucial for osmotic control.
authors.affiliation: Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907-1392, USA.
authors.forename: X
authors.initials: X
authors.lastname: Zhang
authors.forename: S A
authors.initials: SA
authors.lastname: Fletcher
authors.forename: L N
authors.initials: LN
authors.lastname: Csonka
mesh_terms: D026905:Amino Acid Transport Systems; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D016297:Mutagenesis, Site-Directed; D011401:Promoter Regions, Genetic; D012486:Salmonella typhimurium / Q000235:genetics*
chemical_list: D026905:Amino Acid Transport Systems; D001426:Bacterial Proteins; D002352:Carrier Proteins; C060774:ProU protein, Bacteria