Patch-clamp study on membrane properties and transmitter activated currents of rabbit area postrema neurons.
Using the patch-clamp technique in combination wit sliced tissue preparation the membrane properties of newborn rabbit area postrema neurons were investigated. The neurons responded upon depolarization with a fast Na+-current followed by an inactivating and non-inactivating K+-current. GABA-activated currents were investigated resulting in a large C1-(-)conductance, indicating the expression of GABAA-receptors. The expression of glutamate receptor mRNA was studied by in situ hybridization and electrophysiological measurements of these receptors by means of the patch-clamp technique. As a main result it was found that ionotropic glutamate receptors in the area postrema are composed of "flop" variants of the GluA-, GluB- and GluC-subunits.
Jahn
K
K
Neurologische Klinik, Technischen Universität München, Germany.
Bufler
J
J
Weindl
A
A
Arzberger
T
T
Hatt
H
H
D000818:Animals; D001483:Base Sequence; D001933:Brain Stem / Q000166:cytology / Q000502:physiology; D002462:Cell Membrane / Q000502:physiology; D002552:Cerebral Ventricles / Q000166:cytology / Q000502:physiology*; D018690:Excitatory Amino Acid Agonists / Q000494:pharmacology; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology; D006651:Histocytochemistry; D017403:In Situ Hybridization; D066298:In Vitro Techniques; D015640:Ion Channel Gating / Q000502:physiology; D007473:Ion Channels / Q000187:drug effects / Q000502:physiology*; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D008969:Molecular Sequence Data; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D018377:Neurotransmitter Agents / Q000502:physiology*; D018408:Patch-Clamp Techniques; D015347:RNA Probes; D011817:Rabbits; D018079:Receptors, GABA / Q000378:metabolism / Q000502:physiology; D017470:Receptors, Glutamate / Q000378:metabolism / Q000502:physiology
D018690:Excitatory Amino Acid Agonists; D018691:Excitatory Amino Acid Antagonists; D007473:Ion Channels; D018377:Neurotransmitter Agents; D015347:RNA Probes; D018079:Receptors, GABA; D017470:Receptors, Glutamate