title: YAC and cosmid contigs spanning the Batten disease (CLN3) region at 16p12.1-p11.2.
abstract: A yeast artificial chromosome (YAC) contig has been constructed in 16p12.1-p11.2 that encompasses three loci (D16S288, D16S299, and D16S298) closely linked to the gene causing Batten disease or juvenile-onset neuronal ceroid lipofuscinosis (CLN3). The physical map has been ordered using 42 sequence tagged sites. Four genes, interleukin-4 receptor (IL4R), phenol-preferring phenol sulfotransferase (STP), monoamine-preferring phenol sulfotransferase (STM), and sialophorin (SPN), have been mapped to the YAC contig. A partial genomic restriction map has been constructed to confirm the order and distances between D16S298, predicted to be the locus closest to CLN3. The overlapping genomic clones are a valuable resource for cloning the Batten gene (CLN3) and other genes in the region.
authors.affiliation: Department of Paediatrics, UCL Medical School, Rayne Institute, London, United Kingdom.
authors.forename: I E
authors.initials: IE
authors.lastname: Järvelä
authors.forename: H M
authors.initials: HM
authors.lastname: Mitchison
authors.forename: A M
authors.initials: AM
authors.lastname: O'Rawe
authors.forename: P B
authors.initials: PB
authors.lastname: Munroe
authors.forename: P E
authors.initials: PE
authors.lastname: Taschner
authors.forename: N
authors.initials: N
authors.lastname: de Vos
authors.forename: T J
authors.initials: TJ
authors.lastname: Lerner
authors.forename: K L
authors.initials: KL
authors.lastname: D'Arigo
authors.forename: D F
authors.initials: DF
authors.lastname: Callen
authors.forename: A D
authors.initials: AD
authors.lastname: Thompson
mesh_terms: D015703:Antigens, CD / Q000235:genetics; D015239:Arylsulfotransferase / Q000235:genetics; D001483:Base Sequence; D002874:Chromosome Mapping; D018244:Chromosomes, Artificial, Yeast; D002885:Chromosomes, Human, Pair 16; D003360:Cosmids; D017931:DNA Primers; D008040:Genetic Linkage; D005819:Genetic Markers; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D015847:Interleukin-4 / Q000378:metabolism; D007527:Isoenzymes / Q000235:genetics; D051917:Leukosialin; D008969:Molecular Sequence Data; D009472:Neuronal Ceroid-Lipofuscinoses / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D018123:Receptors, Interleukin / Q000235:genetics; D019948:Receptors, Interleukin-4; D015183:Restriction Mapping; D016324:Sequence Tagged Sites; D012795:Sialoglycoproteins / Q000235:genetics
chemical_list: D015703:Antigens, CD; D017931:DNA Primers; D005819:Genetic Markers; D007527:Isoenzymes; D051917:Leukosialin; D018123:Receptors, Interleukin; D019948:Receptors, Interleukin-4; C465014:SPN protein, human; D012795:Sialoglycoproteins; D015847:Interleukin-4; D015239:Arylsulfotransferase