A kinase/phosphatase system involving the protooncogene Lck is necessary for inducible modulation of Sob 1 during T cell activation.
The phosphotyrosine containing protein Sob 1 regulates inducibility of a DNA binding complex (Band A) at the SRE of c-fos during T cell activation. In the present study, Sob 1 was regulated by activation of T cells through the T cell antigen receptor. Inhibition of protein tyrosine kinases (PTK) inactivated Sob 1 function and allowed the Band A DNA-protein complex to form. Inhibition of protein tyrosine phosphatases (PTP) caused constitutive activation of Sob 1 function and prevented induction of Band A at the SRE. Thus, Sob 1 is regulated by a constitutive PTP/PTK system. J.CaM 1 cells that lack a functional form of the PTK Lck are unable to induce Band A through the T cell antigen receptor. J.CaM 1 cells reconstituted with Lck seem to induce Band A normally. Thus, functional Lck is required for regulation of Sob 1.
Benson
B A
BA
Department of Pharmacology and Toxicology, University of Kansas, Lawrence, Kansas 66045-2505, USA.
Butler
Y X
YX
Ackerman
M
M
Benedict
S H
SH
D001483:Base Sequence; D004247:DNA / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism; D008213:Lymphocyte Activation; D019860:Lymphocyte Specific Protein Tyrosine Kinase p56(lck); D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D010750:Phosphoproteins / Q000378:metabolism*; D011485:Protein Binding; D017027:Protein Tyrosine Phosphatases / Q000378:metabolism*; D011518:Proto-Oncogene Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D011948:Receptors, Antigen, T-Cell / Q000378:metabolism; D012045:Regulatory Sequences, Nucleic Acid; D013601:T-Lymphocytes / Q000201:enzymology / Q000276:immunology* / Q000378:metabolism; D014407:Tumor Cells, Cultured
D004268:DNA-Binding Proteins; D009838:Oligodeoxyribonucleotides; D010750:Phosphoproteins; D011518:Proto-Oncogene Proteins; D011948:Receptors, Antigen, T-Cell; D004247:DNA; D019860:Lymphocyte Specific Protein Tyrosine Kinase p56(lck); D017027:Protein Tyrosine Phosphatases