title: Long-term potentiation in cultures of single hippocampal granule cells: a presynaptic form of plasticity.
abstract: We have explored the mechanisms of mossy fiber long-term potentiation (LTP) at autapses in single-cell cultures of guinea pig hippocampal dentate granule cells. L-AP4-sensitive, but not insensitive, cells responded to a brief tetanus with a sustained potentiation in the synaptic responses. The induction of this LTP appeared identical to that observed in hippocampal mossy fiber synapses in situ, in that it required a rise in presynaptic Ca2+ and activation of protein kinase A. Its expression also appeared to be presynaptic and was due, at least in part, to events that occurred after the entry of Ca2+ and to the switching on of previously silent release sites.
authors.affiliation: Department of Cellular and Molecular Pharmacology, University of California, San Francisco 94143, USA.
authors.forename: G
authors.initials: G
authors.lastname: Tong
authors.forename: R C
authors.initials: RC
authors.lastname: Malenka
authors.forename: R A
authors.initials: RA
authors.lastname: Nicoll
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D016291:Dizocilpine Maleate / Q000494:pharmacology; D006168:Guinea Pigs; D006624:Hippocampus / Q000187:drug effects / Q000502:physiology*; D017774:Long-Term Potentiation / Q000187:drug effects / Q000502:physiology*; D016202:N-Methylaspartate / Q000494:pharmacology; D009473:Neuronal Plasticity / Q000187:drug effects / Q000502:physiology*; D013997:Time Factors
chemical_list: D016202:N-Methylaspartate; D016291:Dizocilpine Maleate