title: 45-kDa GTP-binding protein from rat olfactory epithelium: purification, characterization and localization.
abstract: The rat olfactory epithelium contains a specific water-soluble 45-kDa protein. This protein is recognized by anti-peptide antibodies which react with alpha-subunits of the known G-proteins. The 45-kDa protein has been isolated using DEAE-chromatography and gel-exclusion chromatography. The content of 45-kDa protein is about 2% of the total soluble proteins of the olfactory mucosa and it is located at the mucociliary surface. According to photo-affinity labeling, the 45-kDa protein possesses a high affinity to GTP and exhibits low GTP hydrolytic activity. The functions of the 45-kDa protein are discussed.
authors.affiliation: Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region, Russia.
authors.forename: V I
authors.initials: VI
authors.lastname: Novoselov
authors.forename: I V
authors.initials: IV
authors.lastname: Peshenko
authors.forename: V A
authors.initials: VA
authors.lastname: Evdokimov
authors.forename: J V
authors.initials: JV
authors.lastname: Nikolaev
authors.forename: E A
authors.initials: EA
authors.lastname: Matveeva
authors.forename: E E
authors.initials: EE
authors.lastname: Fesenko
mesh_terms: D000345:Affinity Labels; D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies; D004848:Epithelium / Q000737:chemistry; D019204:GTP-Binding Proteins / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification*; D006160:Guanosine Triphosphate / Q000737:chemistry; D015151:Immunoblotting; D008297:Male; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009831:Olfactory Mucosa / Q000737:chemistry*; D010761:Phosphorus Radioisotopes; D011817:Rabbits; D051381:Rats; D017208:Rats, Wistar; D014420:Turbinates
chemical_list: D000345:Affinity Labels; D000906:Antibodies; D010761:Phosphorus Radioisotopes; D006160:Guanosine Triphosphate; D019204:GTP-Binding Proteins