Growth hormone modulates IL-alpha and IFN-gamma release by murine splenocytes activated by LPS or porins of Salmonella typhimurium.
The effect of growth hormone (GH) on the release of IL-1alpha and IFN-gamma from murine splenocytes was investigated. Their release from splenocytes activated by Salmonella enterica serovar Typhimurium lipopolysaccharide (LPS) 0.5 microg/ml was increased by c. 65% in the presence of GH 100 pg/ml. With splenocytes activated by S. Typhimurium porins 5 microg/ml, GH increased the production of both IL-1alpha and IFN-gamma by c. 56%. Polymyxin treatment abolished the cytokine-releasing activity of LPS but had no effect on the activity of the porin preparation.
Sommese
L
L
Istituto di Microbiologia, Facoltà di Medicina e Chirurgia, Seconda Università degli Studi di Napoli, Italy.
Donnarumma
G
G
de l'Ero
C
C
Marcatili
A
A
Vitiello
M
M
Galdiero
M
M
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D015870:Gene Expression; D013006:Growth Hormone / Q000494:pharmacology*; D007371:Interferon-gamma / Q000235:genetics / Q000378:metabolism*; D007375:Interleukin-1 / Q000235:genetics / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000737:chemistry / Q000494:pharmacology*; D008213:Lymphocyte Activation / Q000187:drug effects; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D016133:Polymerase Chain Reaction; D011113:Polymyxins / Q000494:pharmacology; D018272:Porins / Q000737:chemistry / Q000494:pharmacology*; D012333:RNA, Messenger / Q000096:biosynthesis; D012486:Salmonella typhimurium / Q000276:immunology; D013154:Spleen / Q000166:cytology* / Q000187:drug effects / Q000276:immunology; D013552:Swine
D000900:Anti-Bacterial Agents; D007375:Interleukin-1; D008070:Lipopolysaccharides; D011113:Polymyxins; D018272:Porins; D012333:RNA, Messenger; D007371:Interferon-gamma; D013006:Growth Hormone