Cell-cycle-dependent expression of the STK-1 gene encoding a novel murine putative protein kinase.
We have cloned a novel putative serine/threonine kinase-encoding gene, designed STK-1, from murine embryonic stem (ES) cell and testis cDNA libraries. The kinase most closely related to STK-1 is Xenopus laevis XLP46 protein kinase which shows 71% amino-acid identity to STK-1 between their kinase domains. Nevertheless, STK-1 is conserved throughout phylogeny with hybridizing sequences being detected in DNA from mammals, amphibians, insects and yeast. STK-1 mRNA is detected in testis, intestine and spleen, tissues that contain a large number of proliferating cells, but not in other tissues. All cell lines tested expressed STK-1 mRNA with levels being dependent upon proliferation rates. In NIH 3T3 cells, STK-1 is expressed in a cell-cycle-dependent fashion. These findings suggest a role for STK-1 in cell growth.
Niwa
H
H
Department of Developmental Genetics, Kumamoto University School of Medicine, Japan.
Abe
K
K
Kunisada
T
T
Yamamura
K
K
D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D064107:Aurora Kinase B; D064127:Aurora Kinases; D001483:Base Sequence; D002453:Cell Cycle; D002455:Cell Division; D002460:Cell Line; D017124:Conserved Sequence; D018076:DNA, Complementary; D015870:Gene Expression / Q000235:genetics*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D017346:Protein Serine-Threonine Kinases / Q000737:chemistry / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D013737:Testis / Q000502:physiology
D018076:DNA, Complementary; C576924:Aurkb protein, mouse; D064107:Aurora Kinase B; D064127:Aurora Kinases; D017346:Protein Serine-Threonine Kinases