Expression of cDNAs encoding mouse cardiac troponin T isoforms: characterization of a large sample of independent clones.
We have isolated 52 mouse cardiac troponin-T-encoding cDNA clones (TnT) by specific antibody screening of a lambda ZAPII expression library. Sequencing data from the large sample of independent cDNAs demonstrated relationships among the expression of four alternatively-spliced exons of the cardiac TnT gene, producing seven classes of cDNAs encoding four protein isoforms differing in two variable regions. In the N-terminal variable region and next to the embryonic-specific exon 4, an alternatively spliced exon 3a was identified in 20% of the adult isoforms. The alternatively spliced exon 12, corresponding to a central variable region between the two functional domains of TnT, was found in approx. 79% of the 52 mouse cardiac TnT cDNAs with a single base mutation completely abolishing the splicing at an internal acceptor site. Three novel alternative splicing acceptor sites in the 5'-untranslated portion of exon 2 have been identified with different frequencies.
Jin
J P
JP
Department of Medical Biochemistry, University of Calgary, Alberta, Canada. jjin@acs.ucalgary.ca
Wang
J
J
Zhang
J
J
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002645:Chickens; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004742:Enhancer Elements, Genetic; D005091:Exons; D015870:Gene Expression; D015723:Gene Library; D006801:Humans; D051379:Mice / Q000235:genetics*; D008969:Molecular Sequence Data; D009206:Myocardium / Q000378:metabolism*; D017354:Point Mutation; D011110:Polymorphism, Genetic; D011817:Rabbits; D051381:Rats; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D012756:Sheep; D014336:Troponin / Q000096:biosynthesis / Q000235:genetics*; D020107:Troponin T
D018076:DNA, Complementary; D014336:Troponin; D020107:Troponin T