Sequence comparison of the VP7 gene encoding the outer capsid glycoprotein among animal and human group C rotaviruses.
The nucleotide sequences of the outer capsid glycoprotein (VP7) genes from the Shintoku bovine and the HF and WH porcine group C rotaviruses were determined and compared with those of the published corresponding genes from the Cowden porcine and Ehime human group C rotaviruses. The VP7 genes of all 5 strains were 1063 nucleotides in length and possess one open reading frame encoding a polypeptide of 332 amino acids. Comparative analysis of the deduced amino acid sequences indicated that 85.2-97.0% identity was observed for the VP7 of the serotypically related strains of group C rotaviruses (Cowden, WH and Ehime) whereas 69.9-74.7% identity was observed among the serotypically distinct strains (Shintoku; Cowden, WH and Ehime; and HF). At least 8 variable regions in the VP7 were recognized among serotypically distinct strains, and these locations were similar to those of the variable regions in the VP7 of group A rotaviruses.
Tsunemitsu
H
H
Food Animal Health Research Program, Ohio State University, Wooster, USA.
Jiang
B
B
Saif
L J
LJ
D000818:Animals; D000956:Antigens, Viral; D001483:Base Sequence; D002213:Capsid / Q000235:genetics*; D036022:Capsid Proteins; D002417:Cattle; D002460:Cell Line; D017931:DNA Primers; D004279:DNA, Viral; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral; D012401:Rotavirus / Q000145:classification / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D012703:Serotyping; D013552:Swine
D000956:Antigens, Viral; D036022:Capsid Proteins; D017931:DNA Primers; D004279:DNA, Viral; D012367:RNA, Viral; C050779:VP7 protein, Rotavirus