Monocyte activity in Behçet's disease.
Monocytes obtained from patients with Behçet's disease (BD) were examined for differentiation markers (expression of CD14 and antigens reacting with monoclonal antibodies 25F9 and G16/1) and for expression of selected adhesion molecules. There was significantly raised expression of the CD14 molecule, and increased staining with 25F9 and G16/1 antibodies in monocytes obtained from patients with BD. A monocyte activation marker, soluble CD14, was also found to be raised in patients' serum compared with normal serum (8.1 +/- 9.2 vs 1.4 +/- 0.7 micrograms/ml). Furthermore, BD patients' monocyte culture supernatants caused significantly increased adhesion of normal neutrophils to endothelial cell monolayers in vitro. All these findings show that BD patients' monocytes are active in vivo and produce a number of pro-inflammatory cytokines which may play a role in the chronic inflammation found in these patients.
Sahin
S
S
Department of Immunology, Marmara Medical School, Istanbul, Turkey.
Lawrence
R
R
Direskeneli
H
H
Hamuryudan
V
V
Yazici
H
H
Akoğlu
T
T
D001528:Behcet Syndrome / Q000276:immunology*; D018821:CD18 Antigens / Q000378:metabolism; D002448:Cell Adhesion; D002478:Cells, Cultured; D004727:Endothelium / Q000166:cytology; D005434:Flow Cytometry; D006801:Humans; D018950:Lipopolysaccharide Receptors / Q000378:metabolism; D016169:Lymphocyte Function-Associated Antigen-1 / Q000378:metabolism; D016177:Macrophage-1 Antigen / Q000378:metabolism; D009000:Monocytes / Q000276:immunology* / Q000502:physiology; D009504:Neutrophils / Q000166:cytology
D018821:CD18 Antigens; D018950:Lipopolysaccharide Receptors; D016169:Lymphocyte Function-Associated Antigen-1; D016177:Macrophage-1 Antigen