TFIIH functions in regulating transcriptional elongation by RNA polymerase II in Xenopus oocytes.
We investigated the role of TFIIH in transcription by RNA polymerase II (pol II) in vivo by microinjection of antibodies against this factor into Xenopus oocytes. Five different antibodies directed against four subunits of TFIIH were tested for effects on transcription of coinjected human immunodeficiency virus type 2 and c-myc templates. Each of these antibodies severely reduced the efficiency of elongation through human immunodeficiency virus type 2 and c-myc terminator elements. In contrast, an anti-TFIIB antibody did not inhibit elongation. Anti-TFIIH antibodies also had a much smaller inhibitory effect on total transcription than did anti-TFIIB or anti-pol II large subunit. Three inhibitors of TFIIH kinase activity, H-7, H-8, and dichlororibofuranosylbenzimidazole (DRB), inhibited elongation similarly to anti-TFIIH antibodies. These results strongly suggest a role for TFIIH in the stimulation of transcriptional elongation in vivo.
Yankulov
K Y
KY
Amgen Institute, Toronto, Ontario, Canada.
Pandes
M
M
McCracken
S
S
Bouchard
D
D
Bentley
D L
DL
D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D004265:DNA Helicases / Q000378:metabolism; D004260:DNA Repair; D004268:DNA-Binding Proteins / Q000276:immunology / Q000378:metabolism; D029721:Drosophila Proteins; D005260:Female; D005786:Gene Expression Regulation; D016259:Genes, myc; D015498:HIV-2 / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D009865:Oocytes / Q000502:physiology*; D011506:Proteins / Q000276:immunology / Q000378:metabolism; D012319:RNA Polymerase II / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D013698:Templates, Genetic; D013728:Terminator Regions, Genetic; D051758:Transcription Factor TFIIH; D014157:Transcription Factors / Q000276:immunology / Q000378:metabolism*; D019473:Transcription Factors, TFII; D014158:Transcription, Genetic; D014982:Xenopus laevis; D051759:Xeroderma Pigmentosum Group D Protein
D000906:Antibodies; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D011506:Proteins; D011994:Recombinant Proteins; D014157:Transcription Factors; D019473:Transcription Factors, TFII; D051758:Transcription Factor TFIIH; C078672:hay protein, Drosophila; D012319:RNA Polymerase II; D004265:DNA Helicases; D051759:Xeroderma Pigmentosum Group D Protein; C497292:ERCC2 protein, human