Use of peptide immunization for porcine insulin of a very high homology with a host protein.
An anti-peptide antibody was obtained against a peptide corresponding to the 12 amino acids of the C-terminal region of porcine insulin B chain. The adsorption characteristics of this antibody were compared with those of anti-porcine insulin and anti-sheep insulin antibodies. Immunization of rabbits using the peptide corresponding to the C-terminal region of porcine insulin B chain produced an anti-peptide antibody which reacted with native porcine insulin with a much higher association constant than that of an anti-porcine insulin antibody. In addition, this immunization technique did not cause any observable physiologically harmful effects, such as hypoglycemia, in the immunized rabbits. Thus, peptide immunization may be a useful strategy when target proteins have biological activity and/or toxicity, and also have a very high degree of homology with the corresponding proteins of the immunized animal, which may inhibit the production of antibodies with a high association constant.
Katoh
S
S
Department of Synthetic Chemistry and Biological Chemistry, Kyoto University, Japan.
Terashima
M
M
Tomioka
K
K
Hikida
N
N
D000327:Adsorption; D000595:Amino Acid Sequence; D000818:Animals; D000915:Antibody Affinity; D003429:Cross Reactions; D007114:Immunization; D007328:Insulin / Q000737:chemistry / Q000276:immunology*; D007330:Insulin Antibodies / Q000737:chemistry; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000276:immunology*; D011817:Rabbits; D051381:Rats; D017386:Sequence Homology, Amino Acid; D013552:Swine
D007328:Insulin; D007330:Insulin Antibodies; D010455:Peptides