Isolation of cDNAs and tissue specific expression of ovine glucose transporters.
The facilitative glucose transporters are a family of proteins responsible for the transmembrane transport of glucose and other hexose sugars (1,2). In mammals, the seven glucose transporter isoforms display a characteristic tissue distribution reflecting the physiological requirement and metabolism of glucose. This report describes the isolation and sequencing of the full length ovine GLUT-3 cDNA and the tissue distribution of ovine GLUT-1 and GLUT-3 mRNA. The ovine GLUT-3 cDNA is 3854 base pairs and the coding nucleotides show 82% and 79% homology with the human and mouse GLUT-3 sequences respectively. In addition, a reverse transcriptase-polymerase chain reaction strategy is described for the rapid isolation of mammalian cDNA subclones for GLUT-1, GLUT-2 and GLUT-4. This method has been used to isolate the corresponding ovine subclones.
Bennett
B L
BL
Dairy Science Group, AgResearch, Ruakura Research Centre, Hamilton, New Zealand.
Prosser
C G
CG
Grigor
M R
MR
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000302:isolation & purification*; D005947:Glucose / Q000378:metabolism*; D051272:Glucose Transporter Type 1; D051274:Glucose Transporter Type 3; D051275:Glucose Transporter Type 4; D008969:Molecular Sequence Data; D009004:Monosaccharide Transport Proteins / Q000737:chemistry / Q000235:genetics*; D009124:Muscle Proteins; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger / Q000032:analysis; D017386:Sequence Homology, Amino Acid; D012756:Sheep; D014018:Tissue Distribution
D018076:DNA, Complementary; D051272:Glucose Transporter Type 1; D051274:Glucose Transporter Type 3; D051275:Glucose Transporter Type 4; D009004:Monosaccharide Transport Proteins; D009124:Muscle Proteins; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D005947:Glucose