Relevance of the arginine transport activity to the nitric oxide synthesis in mouse peritoneal macrophages stimulated with bacterial lipopolysaccharide.
Transport of arginine and production of nitrite have been investigated in mouse peritoneal macrophages stimulated with bacterial lipopolysaccharide (LPS). The arginine transport activity was induced by LPS at very low concentration (maximally induced at 1 ng/ml), whereas much higher concentration of LPS was required for the induction of nitrite production. Arginine was more concentrated in the cells when its transport activity was induced. Lysine, which is a competitive inhibitor of the transport of arginine, neutralized the concentrative effect of the induced transport activity and thus inhibited the nitrite production. Induction of the arginine transport activity seems to be prerequisite to the enhanced synthesis of nitric oxide in activated macrophages.
Shibazaki
T
T
Department of Biochemistry, University of Tsukuba, Ibaraki, Japan.
Fujiwara
M
M
Sato
H
H
Fujiwara
K
K
Abe
K
K
Bannai
S
S
D026905:Amino Acid Transport Systems; D000818:Animals; D001120:Arginine / Q000378:metabolism*; D001692:Biological Transport / Q000187:drug effects / Q000502:physiology; D002352:Carrier Proteins / Q000378:metabolism*; D002478:Cells, Cultured; D003470:Culture Media; D005110:Extracellular Space / Q000737:chemistry / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation / Q000235:genetics; D007424:Intracellular Fluid / Q000737:chemistry / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008239:Lysine / Q000494:pharmacology; D017737:Macrophages, Peritoneal / Q000378:metabolism*; D051379:Mice; D009569:Nitric Oxide / Q000096:biosynthesis*; D009573:Nitrites / Q000378:metabolism
D026905:Amino Acid Transport Systems; D002352:Carrier Proteins; D003470:Culture Media; D008070:Lipopolysaccharides; D009573:Nitrites; D009569:Nitric Oxide; D001120:Arginine; D008239:Lysine