Activation of Src-family protein tyrosine kinases and phosphatidylinositol 3-kinase in 3T3-L1 mouse preadipocytes by interleukin-11.
Tyrosine phosphorylation is an early event of interleukin-11 (IL-11)-mediated signal transduction. The IL-11-responsive cell line 3T3-L1 (mouse preadipocytes) was tested for the presence of Src-family protein tyrosine kinases (PTKs). Only p62yes, p59fyn, and p60src were found to be expressed. Immune-complex kinase reactions using the artificial substrate enolase showed that upon stimulation with IL-11, p62yes and p60src were activated, but p59fyn did not appear to be activated. These results correlate with antiphosphotyrosine (APT) immunoblots. IL-11-stimulated cells also revealed increased phosphatidylinositol 3-kinase (PI3K) activity. Increased activity of PI3K was found to be associated with tyrosine phosphorylated proteins and p62yes, after IL-11 treatment. Immunoprecipitation studies with anti-PI3K revealed for the first time that p62yes associated with PI3K in response to IL-11. To summarize, IL-11 activates p60src, p62yes and PI3K but not p59fyn, and this activation of p62yes leads to its association with PI3K and PI3K activation.
Fuhrer
D K
DK
Department of Medicine (Hematology/Oncology) and Walther Oncology Center, Indiana University School of Medicine, Indianapolis 46202, USA.
Yang
Y C
YC
D016475:3T3 Cells / Q000187:drug effects* / Q000201:enzymology; D000818:Animals; D004789:Enzyme Activation / Q000187:drug effects; D016391:Genes, src; D017370:Interleukin-11 / Q000494:pharmacology*; D015850:Interleukin-6 / Q000494:pharmacology; D051379:Mice; D005810:Multigene Family; D019869:Phosphatidylinositol 3-Kinases; D010751:Phosphopyruvate Hydratase / Q000378:metabolism; D010766:Phosphorylation / Q000187:drug effects; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000378:metabolism*; D011499:Protein Processing, Post-Translational / Q000187:drug effects*; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D051076:Proto-Oncogene Proteins c-fyn; D051575:Proto-Oncogene Proteins c-yes; D016392:Proto-Oncogene Proteins pp60(c-src) / Q000378:metabolism*; D011994:Recombinant Proteins / Q000494:pharmacology; D015398:Signal Transduction; D019061:src-Family Kinases
D017370:Interleukin-11; D015850:Interleukin-6; D011518:Proto-Oncogene Proteins; D011994:Recombinant Proteins; D019869:Phosphatidylinositol 3-Kinases; D017853:Phosphotransferases (Alcohol Group Acceptor); D011505:Protein-Tyrosine Kinases; C494960:Fyn protein, mouse; D051076:Proto-Oncogene Proteins c-fyn; D051575:Proto-Oncogene Proteins c-yes; D016392:Proto-Oncogene Proteins pp60(c-src); C496639:Yes1 protein, mouse; D019061:src-Family Kinases; D010751:Phosphopyruvate Hydratase