title: Fluorescence in situ hybridization: a brief review.
abstract: Fluorescence in situ hybridization (FISH) is used for many purposes, including analysis of chromosomal damage, gene mapping, clinical diagnostics, molecular toxicology and cross-species chromosome homology. FISH allows an investigator to identify the presence and location of a region of cellular DNA or RNA within morphologically preserved chromosome preparations, fixed cells or tissue sections. This report describes in situ hybridization, and discusses the past, present and future applications of this method for genetic analysis and molecular toxicology.
authors.affiliation: Biology and Biotechnology Research Program, Lawrence Livermore National Laboratory, Livermore, California, USA.
authors.forename: R R
authors.initials: RR
authors.lastname: Swiger
authors.forename: J D
authors.initials: JD
authors.lastname: Tucker
mesh_terms: D000818:Animals; D002869:Chromosome Aberrations; D004247:DNA / Q000235:genetics* / Q000378:metabolism; D015342:DNA Probes; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D016133:Polymerase Chain Reaction; D012313:RNA / Q000235:genetics* / Q000378:metabolism; D013696:Temperature
chemical_list: D015342:DNA Probes; D012313:RNA; D004247:DNA