title: Non-radioisotopic and semi-quantitative procedure for terminal repeat amplification protocol.
abstract: We have used fluorescence-labeled primers and an auto-sequencer to detect telomerase activity quickly and easily. The current procedure is superior to the original telomeric repeat amplification protocol in several respects: 1) the result is obtained in real time during electrophoresis, 2) semi-quantitative results are possible without using a photo-capture system, and 3) no radioisotope is needed.
authors.affiliation: First Department of Internal Medicine, Tokyo Medical College, Japan.
authors.forename: J H
authors.initials: JH
authors.lastname: Ohyashiki
authors.forename: K
authors.initials: K
authors.lastname: Ohyashiki
authors.forename: T
authors.initials: T
authors.lastname: Sano
authors.forename: K
authors.initials: K
authors.lastname: Toyama
mesh_terms: D001483:Base Sequence; D017931:DNA Primers; D005456:Fluorescent Dyes; D018922:HL-60 Cells; D006801:Humans; D008969:Molecular Sequence Data; D021141:Nucleic Acid Amplification Techniques; D012091:Repetitive Sequences, Nucleic Acid; D019098:Telomerase / Q000378:metabolism
chemical_list: D017931:DNA Primers; D005456:Fluorescent Dyes; D019098:Telomerase