Microsatellite sequences in a conifer, Pinus sylvestris.
Scots pine (Pinus sylvestris) genomic libraries were constructed and screened with oligonucleotides probes (GT)10, (CT)10, and (AT)10. Eight microsatellites were identified from 6000 clones screened. The longest microsatellite stretch found, (CT)9(N)21(AT)24, was amplified from bud and single pollen grain samples. In order to clarify the complex amplification pattern revealed, two PCR products were sequenced. The size differences were caused both by varying repeat numbers of the microsatellite stretches and by differences in other parts of the amplified sequence. This kind of complex molecular basis of microsatellite amplification within a species has been previously reported. Microsatellite sequences were used as PCR primers to detect polymorphisms and to estimate the abundance of microsatellites.
Kostia
S
S
Department of Biosciences, University of Helsinki, Finland.
Varvio
S L
SL
Vakkari
P
P
Pulkkinen
P
P
D001482:Base Composition; D001483:Base Sequence; D016172:DNA Fingerprinting; D018744:DNA, Plant / Q000235:genetics*; D005784:Gene Amplification; D018895:Microsatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data; D041605:Pinus sylvestris; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA
D018744:DNA, Plant