Two-peak kinetic curve of the chemiluminescence in phorbol-induced macrophage.
Phorbol-1,2-myristate-1,3-acetate can induce rat macrophage to emit chemiluminescence. The kinetic curve of the chemiluminescence clearly showed two peaks when a proper ratio of phorbol-1,2-myristate-1,3-acetate and macrophage were mixed. It was proved that the first one mainly came from the oxygen free radicals and the second one from NO generated in the phorbol-1,2-myristate-1,3-acetate stimulated cells by using the substitute and inhibitor of NO synthase.
Li
H
H
Institute of Biophysics, Academia Sinica, Beijing, China.
Zhao
B L
BL
Hou
J W
JW
Xin
W J
WJ
D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives / Q000494:pharmacology; D002460:Cell Line; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005609:Free Radicals / Q000378:metabolism; D007700:Kinetics; D008163:Luminescent Measurements; D008264:Macrophages / Q000187:drug effects / Q000502:physiology*; D009569:Nitric Oxide / Q000096:biosynthesis; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors / Q000378:metabolism; D019335:Nitroarginine; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D004791:Enzyme Inhibitors; D005609:Free Radicals; D019335:Nitroarginine; D009569:Nitric Oxide; D001120:Arginine; D019001:Nitric Oxide Synthase; D013755:Tetradecanoylphorbol Acetate