Bumetanide is not transported by the Ntcp or by the oatp: evidence for a third organic anion transporter in rat liver cells.
The loop diuretic bumetanide which inhibits hepatic bile acid uptake competitively according to its transport kinetics has been proposed to serve as a substrate of a multispecific bile acid transport system in liver parenchymal cells. However, when the in vitro transcripts of two cloned hepatic bile acid uptake carriers, the Ntcp (Na+/taurocholate cotransporting polypeptide) and the oatp (organic anion transporting polypeptide), was expressed for three days in Xenopus laevis oocytes [3H]bumetanide uptake was not increased although bile acid uptake was stimulated. The data presented show that bumetanide is taken up by a third organic anion transport system which is different from the cloned bile acid transporters.
Horz
J A
JA
Institute of Pharmacology and Toxicology, Giessen, Germany.
Honscha
W
W
Petzinger
E
E
D018528:ATP-Binding Cassette Transporters; D000818:Animals; D027321:Anion Transport Proteins; D001692:Biological Transport; D002034:Bumetanide / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism*; D004232:Diuretics / Q000378:metabolism*; D006000:Glycocholic Acid / Q000378:metabolism; D017136:Ion Transport; D008099:Liver / Q000378:metabolism*; D009865:Oocytes / Q000378:metabolism; D016372:RNA, Antisense / Q000378:metabolism / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D012964:Sodium / Q000494:pharmacology; D014982:Xenopus laevis
D018528:ATP-Binding Cassette Transporters; D027321:Anion Transport Proteins; D002352:Carrier Proteins; D004232:Diuretics; D016372:RNA, Antisense; D012333:RNA, Messenger; C038659:taurocholate carrier protein, rat; D002034:Bumetanide; D012964:Sodium; D006000:Glycocholic Acid