title: [Recombinant proteins containing oxytocin oligomeric sequences].
abstract: Expression plasmids were constructed with genes encoding the ILOX3, ILOX6, and ILOX9 recombinant proteins, which contain the C-terminal fragments of trimer, hexamer, or nonamer of oxytocinoyl-Lys. Upon expression in E. coli, all three genes yielded inclusion bodies containing protein products of similar length and heterogeneous in the C-terminal region. It is likely that in the case of the ilox3 gene, the obtained protein mixture includes the full-length product of translation with the C-terminal lysine. In the case of the ilox6 and ilox9 genes, the protein products are formed as the result of a site-specific proteolysis in the regions between the second and the fourth oxytocin units.
authors.forename: A I
authors.initials: AI
authors.lastname: Gurevich
authors.forename: T A
authors.initials: TA
authors.lastname: Kachalina
authors.forename: A L
authors.initials: AL
authors.lastname: Kaiushin
authors.forename: M D
authors.initials: MD
authors.lastname: Korosteleva
authors.forename: K V
authors.initials: KV
authors.lastname: Mal'tsev
authors.forename: O A
authors.initials: OA
authors.lastname: Mirgorodskaia
authors.forename: A I
authors.initials: AI
authors.lastname: Miroshnikov
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D001704:Biopolymers; D004274:DNA, Recombinant; D006868:Hydrolysis; D008969:Molecular Sequence Data; D010121:Oxytocin / Q000235:genetics* / Q000378:metabolism; D010957:Plasmids; D014176:Protein Biosynthesis; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D001704:Biopolymers; D004274:DNA, Recombinant; D011994:Recombinant Proteins; D010121:Oxytocin
vernacular_title: Rekombinantnye belki, soderzhashchie oligomernye posledovatel'nosti oksitotsina.