title: Amyloid beta protein and its 3-kDa fragment are present in the axoplasm fraction of the white matter in human brain.
abstract: Production of the soluble amyloid beta-protein (A beta) precedes abnormal accumulation of A beta amyloid in the brains of subjects with Alzheimer's disease. To determine the cellular source and generating mechanisms of soluble A beta in the human brain, we separated an axoplasm fraction from the cerebral white matter and analyzed it. The axoplasm fraction contained secretory isoforms of beta-amyloid precursor protein (APP) and 11.5 kDa A beta-bearing carboxyl-terminal fragments (CTFs) of APP. Furthermore, soluble 4 kDa A beta and 3 kDa fragments of A beta (p3) were obtained from the axoplasm fraction. These results suggest that amyloidogenic 4 kDa A beta is intracellularly produced in cerebral neurons and carried through the axons in human brain.
authors.affiliation: Department of Medicine (Neurology), Shinshu University School of Medicine, Matsumoto, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Tokuda
authors.forename: F
authors.initials: F
authors.lastname: Kametani
authors.forename: K
authors.initials: K
authors.lastname: Tanaka
authors.forename: N
authors.initials: N
authors.lastname: Sahara
authors.forename: S
authors.initials: S
authors.lastname: Ikeda
authors.forename: N
authors.initials: N
authors.lastname: Yanagisawa
mesh_terms: D000368:Aged; D016229:Amyloid beta-Peptides / Q000032:analysis*; D001369:Axons / Q000737:chemistry* / Q000473:pathology / Q000648:ultrastructure; D001921:Brain / Q000473:pathology*; D001923:Brain Chemistry; D003600:Cytosol / Q000737:chemistry / Q000473:pathology / Q000648:ultrastructure; D005260:Female; D006801:Humans; D008875:Middle Aged; D016874:Neurofibrillary Tangles / Q000473:pathology*; D010446:Peptide Fragments / Q000032:analysis*; D012016:Reference Values
chemical_list: D016229:Amyloid beta-Peptides; D010446:Peptide Fragments