Biliary expression of heat shock protein: a non-specific feature of chronic cholestatic liver diseases.
OBJECTIVE
To analyse the expression of heat shock protein (HSP) 60 in biliary epithelium in auto-immune liver conditions and also in chronic cholestatic and other liver diseases.


METHODS
Hepatic expression of HSP-60 in frozen liver biopsy specimens from patients with primary sclerosing cholangitis (PSC), primary biliary cirrhosis (PBC), auto-immune hepatitis (AIH), obstructive jaundice (LDO), alcoholic liver disease (ALD), and from normal controls was studied by immunohistochemistry using the APAAP technique and confocal laser scanning microscopy.


RESULTS
Increased expression of HSP-60 was demonstrated in the biliary epithelium of patients with PBC, LDO and, to a lesser extent, with PSC. Focal, weaker, biliary epithelial expression of HSP-60 was observed in AIH, ALD and normal liver tissue. Increased expression was also seen on Kupffer cells in LDO and in hepatocytes in areas of piecemeal necrosis in AIH.


CONCLUSIONS
Enhanced biliary expression of HSP-60 is a common feature of chronic biliary disease irrespective of aetiology and is not specific to auto-immune diseases.
Martins
E B
EB
Department of Gastroenterology, John Radcliffe Hospital, Oxford.
Chapman
R W
RW
Marron
K
K
Fleming
K A
KA
D001327:Autoimmune Diseases / Q000378:metabolism*; D001652:Bile Ducts / Q000378:metabolism*; D001660:Biliary Tract Diseases / Q000378:metabolism*; D018834:Chaperonin 60 / Q000378:metabolism*; D015209:Cholangitis, Sclerosing / Q000378:metabolism; D002779:Cholestasis / Q000378:metabolism; D002908:Chronic Disease; D006801:Humans; D007124:Immunoenzyme Techniques; D008105:Liver Cirrhosis, Biliary / Q000378:metabolism; D008107:Liver Diseases / Q000378:metabolism*; D018613:Microscopy, Confocal
D018834:Chaperonin 60