Interaction of Vav with ENX-1, a putative transcriptional regulator of homeobox gene expression.
The proto-oncogene product Vav plays a critical role in hematopoietic signal transduction. By using the yeast two-hybrid system, we identified a novel human protein, ENX-1, which interacts specifically with Vav both in vitro and in vivo. ENX-1 represents the human homolog of the Drosophila Enhancer of zeste gene, a member of the Polycomb group of genes, which are transcriptional regulators of homeobox gene expression. Interaction with ENX-1 suggests that Vav functions as an upstream element in the transcriptional regulation of homeobox genes, known to be important effectors in the hematopoietic system.
Hobert
O
O
Department of Molecular Biology, Max-Planck-Institut für Biochemie, Martinsried, Germany.
Jallal
B
B
Ullrich
A
A
D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D018797:Cell Cycle Proteins; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004330:Drosophila / Q000235:genetics; D029721:Drosophila Proteins; D005786:Gene Expression Regulation; D005801:Genes, Homeobox; D017344:Genes, Insect; D006410:Hematopoiesis / Q000235:genetics; D006801:Humans; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D009687:Nuclear Proteins; D063151:Polycomb Repressive Complex 2; D011485:Protein Binding; D011506:Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D051567:Proto-Oncogene Proteins c-vav; D012097:Repressor Proteins; D012441:Saccharomyces cerevisiae / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction / Q000235:genetics
D018797:Cell Cycle Proteins; D018076:DNA, Complementary; D029721:Drosophila Proteins; C000715470:MAS1 protein, human; D009687:Nuclear Proteins; D011506:Proteins; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins; D051567:Proto-Oncogene Proteins c-vav; D012097:Repressor Proteins; C496618:VAV1 protein, human; C083329:E(z) protein, Drosophila; D063151:Polycomb Repressive Complex 2