Thalidomide increases the synthesis of IL-2 in cultures of human mononuclear cells stimulated with Concanavalin-A, Staphylococcal enterotoxin A, and purified protein derivative.
Thalidomide significantly increases the quantity of extracellular IL-2 in cultures of human mononuclear cells stimulated with mitogens or antigen. Cells from 7 donors exposed for 2 h to 4.0 micrograms/ml of thalidomide and stimulated for 16-18 h with 20 micrograms/ml of Concanavalin-A (Con-A) averaged producing 187 +/- 49% more IL-2 than cells stimulated with Con-A alone. In similar experimental procedures and comparisons the pg/ml of IL-2 secreted by thalidomide-treated cells from five donors stimulated with 50 ng/ml of Staphylococcal enterotoxin A (SEA) increased by 159 +/- 32%, and the pg/ml of IL-2 secreted by thalidomide-treated cells from 2 donors stimulated with 5.0 micrograms/ml of purified protein derivative of Mycobacterium tuberculosis increased by 120 +/- 4%. Thalidomide also significantly increases the quantity of intracellular IL-2 in cells stimulated with mitogens. Cells exposed to thalidomide and stimulated with Con-A had an increase in intracellular IL-2 of 130% after 8 h and 157% after 12 h in culture; cells stimulated with SEA had an increase in intracellular IL-2 of 120% after 8 h and 182% after 12 h in culture. Thalidomide did not alter the percent of lymphocytes expressing the alpha-chain of IL-2 receptor, nor did it significantly increase incorporation of [3H]thymidine by cells.
Shannon
E J
EJ
Immunology Research Department, G.W. Long Hansen's Disease Center, Baton Rouge, LA 70894, USA.
Sandoval
F
F
D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D004768:Enterotoxins / Q000494:pharmacology; D005260:Female; D006801:Humans; D007376:Interleukin-2 / Q000096:biosynthesis*; D007700:Kinetics; D008297:Male; D009000:Monocytes / Q000276:immunology / Q000378:metabolism*; D013792:Thalidomide / Q000494:pharmacology*; D014373:Tuberculin / Q000494:pharmacology
D004768:Enterotoxins; D007376:Interleukin-2; D014373:Tuberculin; D003208:Concanavalin A; C026066:enterotoxin A, Staphylococcal; D013792:Thalidomide