title: The utility of fluorescent in vivo reporter genes in molecular cardiology.
abstract: In vivo reporter genes can be used in different ways in molecular cardiology. In this paper studies are presented using the green fluorescent protein and one of its mutants, S65T-GFP, as in vivo reporter genes. With this new molecular tool we studied cell type specificity of the murine ventricular myosin light chain 2 promoter, positive cell identification prior to patch clamp procedures, and the use of fluorescence activated cell sorting of transiently transfected mammalian cells.
authors.affiliation: Department of Cardiology, Cardiovascular Research Institute Maastricht, The Netherlands.
authors.forename: P A
authors.initials: PA
authors.lastname: Doevendans
authors.forename: K D
authors.initials: KD
authors.lastname: Becker
authors.forename: R H
authors.initials: RH
authors.lastname: An
authors.forename: R S
authors.initials: RS
authors.lastname: Kass
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D015220:Calcium Channels / Q000096:biosynthesis / Q000502:physiology*; D020746:Calcium Channels, L-Type; D002460:Cell Line; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular / Q000379:methods; D005347:Fibroblasts; D049452:Green Fluorescent Proteins; D006352:Heart Ventricles; D006801:Humans; D007668:Kidney; D008164:Luminescent Proteins / Q000032:analysis / Q000096:biosynthesis*; D051379:Mice; D018994:Myosin Light Chains / Q000096:biosynthesis* / Q000235:genetics*; D018408:Patch-Clamp Techniques; D010957:Plasmids; D017354:Point Mutation; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000032:analysis / Q000096:biosynthesis*; D015183:Restriction Mapping; D012680:Sensitivity and Specificity; D014162:Transfection
chemical_list: D015220:Calcium Channels; D020746:Calcium Channels, L-Type; D008164:Luminescent Proteins; D018994:Myosin Light Chains; D011994:Recombinant Proteins; D049452:Green Fluorescent Proteins