Determination of radiation-induced damage in lymphocytes using the micronucleus and microgel electrophoresis 'Comet' assays.
DNA damage assays may be useful as rapid predictors of normal tissue radiosensitivity in clinical samples. We measured in vitro radiation-induced (2 Gy) damage to lymphocytes from cancer patients and normal healthy donors using both the micronucleus and microgel electrophoresis (Comet) assays simultaneously. For damage assessment, there was a good correlation (P < 0.001) between the mean comet lengths and the fraction of cells with comets. There was no correlation with initial damage, determined as the proportion of cells within a sample that formed comets, in comparison with the mean frequency of micronuclei per binucleate cell. However, there appeared to be an association between the determination of repair proficiency in the Comet assay and the mean frequency of micronuclei per binucleate cell in lymphocytes from cancer patients.
Malcolmson
A M
AM
Research Laboratories, Velindre NHS Trust, Whitchurch, Cardiff, U.K.
Davies
G
G
Hanson
J A
JA
Deeley
J O
JO
Gaffney
C C
CC
McGregor
A D
AD
Kerby
I J
IJ
D004247:DNA / Q000528:radiation effects*; D004249:DNA Damage; D004260:DNA Repair; D004587:Electrophoresis, Agar Gel; D005720:Gamma Rays; D006801:Humans; D008214:Lymphocytes / Q000528:radiation effects*; D015162:Micronucleus Tests; D009369:Neoplasms / Q000503:physiopathology
D004247:DNA