Alternative splicing of the Na(+)-Ca2+ exchanger gene, NCX1.
We describe an analysis of the NCX1 gene and show that various tissues express different alternatively spliced forms of the gene. Alternative splicing has been confirmed by the genomic analysis of the Na(+)-Ca2+ exchanger gene. We also describe the Drosophila Na(+)-Ca2+ exchanger as having many of the same structural characteristics of the mammalian exchangers and this locus as possibly undergoing alternative splicing in the same region that has been described in the NCX1 gene. The general structure of the exchangers is similar to that of the alpha-subunit of the (Na(+)+ K+)-A Pase. Finally, sequence comparison of the various molecules demonstrates that structural characteristics of these molecules are more strongly conserved than the primary sequence of these products.
Schulze
D H
DH
Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore 21201, USA.
Kofuji
P
P
Valdivia
C
C
He
S
S
Luo
S
S
Ruknudin
A
A
Wisel
S
S
Kirby
M S
MS
duBell
W
W
Lederer
W J
WJ
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002352:Carrier Proteins / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D017385:Sequence Homology; D012964:Sodium / Q000378:metabolism; D019831:Sodium-Calcium Exchanger; D014162:Transfection
D002352:Carrier Proteins; D019831:Sodium-Calcium Exchanger; D012964:Sodium; D002118:Calcium