Analysis of the amino acid sequences of plant Bowman-Birk inhibitors.
Plant seeds contain a large number of protease inhibitors of animal, fungal, and bacterial origin. One of the well-studied families of these inhibitors is the Bowman-Birk family(BBI). The BBIs from dicotyledonous seeds are 8K, double-headed proteins. In contrast, the 8K inhibitors from monocotyledonous seeds are single headed. Monocots also have a 16K, double-headed inhibitor. We have determined the primary structure of a Bowman-Birk inhibitor from a dicot, horsegram, by sequential edman analysis of the intact protein and peptides derived from enzymatic and chemical cleavage. The 76-residue-long inhibitor is very similar to that of Macrotyloma axillare. An analysis of this inhibitor along with 26 other Bowman-Birk inhibitor domains (MW 8K) available in the SWISSPROT databank revealed that the proteins from monocots and dicots belong to related but distinct families. Inhibitors from monocots show larger variation in sequence. Sequence comparison shows that a crucial disulphide which connects the amino and carboxy termini of the active site loop is lost in monocots. The loss of a reactive site in monocots seems to be correlated to this. However, it appears that this disulphide is not absolutely essential for retention of inhibitory function. Our analysis suggests that gene duplication leading to a 16K inhibitor in monocots has occurred, probably after the divergence of monocots and dicots, and also after the loss of second reactive site in monocots.
Prakash
B
B
Molecular Biophysics Unit, Indian Institute of Science, Bangalore, India.
Selvaraj
S
S
Murthy
M R
MR
Sreerama
Y N
YN
Rao
D R
DR
Gowda
L R
LR
D000595:Amino Acid Sequence; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D016384:Consensus Sequence; D008969:Molecular Sequence Data; D005810:Multigene Family; D010802:Phylogeny; D010944:Plants / Q000235:genetics; D012639:Seeds / Q000737:chemistry; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014358:Trypsin Inhibitor, Bowman-Birk Soybean / Q000737:chemistry*
D014358:Trypsin Inhibitor, Bowman-Birk Soybean