Ti plasmid-encoded genes responsible for catabolism of the crown gall opine mannopine by Agrobacterium tumefaciens are homologs of the T-region genes responsible for synthesis of this opine by the plant tumor.
Agrobacterium tumefaciens NT1 harboring pSaB4, which contains the 14-kb BamHI fragment 4 from the octopine/mannityl opine-type Ti plasmid pTi15955, grew well with agropine (AGR) but slowly with mannopine (MOP) as the sole carbon source. When a second plasmid encoding a dedicated transport system for MOP was introduced, these cells grew well with both AGR and MOP. Transposon insertion mutagenesis and subcloning identified a 5.7-kb region of BamHI fragment 4 that encodes functions required for the degradation of MOP. DNA sequence analysis revealed seven putative genes in this region: mocD (moc for mannityl opine catabolism) and mocE, oriented from right to left, and mocRCBAS, oriented from left to right. Significant identities exist at the nucleotide and derived amino acid sequence levels between these moc genes and the mas genes that are responsible for opine biosynthesis in crown gall tumors. MocD is a homolog of Mas2, the anabolic conjugase encoded by mas2'. MocE and MocC are related to the amino half and the carboxyl half, respectively, of Mas1 (MOP reductase), the second enzyme for MOP biosynthesis. These results indicate that the moc and mas genes evolved from a common origin. MocR and MocS are related to each other and to a putative repressor for the AGR degradation system encoded by the rhizogenic plasmid pRiA4. MocB and MocA are homologs of 6-phosphogluconate dehydratase and glucose-6-phosphate dehydrogenase, respectively. Mutations in mocD and mocE, but not mocC, are suppressed by functions encoded by the chromosome or the 450-kb megaplasmid present in many Agrobacterium isolates. We propose that moc genes derived from genes located elsewhere in the bacterial genome and that the tumor-expressed mas genes evolved from the bacterial moc genes.
Kim
K S
KS
Department of Microbiology, University of Illinois at Urbana-Champaign, 61801, USA.
Farrand
S K
SK
D016960:Agrobacterium tumefaciens / Q000235:genetics* / Q000378:metabolism*; D000595:Amino Acid Sequence; D001483:Base Sequence; D004251:DNA Transposable Elements; D029721:Drosophila Proteins; D005798:Genes, Bacterial; D008353:Mannitol / Q000031:analogs & derivatives* / Q000378:metabolism; D008969:Molecular Sequence Data; D010078:Oxazines / Q000378:metabolism; D010941:Plant Tumors; D010957:Plasmids; D011506:Proteins / Q000235:genetics; D012697:Serine Endopeptidases
D004251:DNA Transposable Elements; D029721:Drosophila Proteins; D010078:Oxazines; D011506:Proteins; D008353:Mannitol; C025992:agropine; C041352:mannopine; C091914:mas protein, Drosophila; D012697:Serine Endopeptidases