A new plasmid-encoded proteic killer gene system: cloning, sequencing, and analyzing hig locus of plasmid Rts1.
A new proteic killer gene system, hig, was identified on the plasmid Rts1. The hig locus consisting of a higA and higB is directly related to the temperature sensitive host cell growth conferred by Rts1. We proved that higB encoding presumably a 92-amino-acid polypeptide inhibited segregation of plasmid free cells, and higA encoding a 104-amino-acid polypeptide suppressed the higB function both in cis and in trans.
Tian
Q B
QB
Department of Bacteriology, Shinshu University School of Medicine, Japan.
Ohnishi
M
M
Tabuchi
A
A
Terawaki
Y
Y
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D029968:Escherichia coli Proteins; D008969:Molecular Sequence Data; D010641:Phenotype; D010957:Plasmids; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D013696:Temperature
D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; C099610:HigA protein, E coli; C099611:HigB protein, E coli; D011994:Recombinant Proteins