Genetic identification of Staphylococcus aureus by polymerase chain reaction using single-base-pair mismatch in 16S ribosomal RNA gene.
Staphylococcus aureus is the most predominant and important pathogen in clinical microbiology. A DNA amplification assay using the polymerase chain reaction (PCR) was designed to identify S. aureus through a single-base-pair mismatch in the sequences of staphylococcal 16S ribosomal RNA (16S rRNA) genes. It was able to detect and identify S. aureus without requiring additional analytical techniques. Twenty-eight staphylococcal and non-staphylococcal strains were tested to verify the specificity of the assay, and only S. aureus strains gave a positive reaction. It may be possible to provide immediate and exact information for the identification of S. aureus.
Saruta
K
K
Department of Laboratory Medicine, Jikei University School of Medicine, Tokyo, Japan.
Hoshina
S
S
Machida
K
K
D001483:Base Sequence; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000032:analysis / Q000235:genetics*; D012680:Sensitivity and Specificity; D012689:Sequence Homology, Nucleic Acid; D013211:Staphylococcus aureus / Q000302:isolation & purification*
D012336:RNA, Ribosomal, 16S