title: +P5 (D1S3309E), a novel target binding site for the Wilms' tumour suppressor 1 (WT1) gene, maps to human chromosome 1q21-->22.
abstract: The Wilms' tumor suppressor 1 gene (WT1) encodes a zinc finger transcription factor critical for normal urogenital development. We have previously isolated a DNA fragment, +P5 (D1S3309E), to which all WT1 protein isoforms bind. Using PCR of a human x rodent somatic cell hybrid mapping panel, together with two-color fluorescence in situ hybridisation of +P5-containing cosmids and previously localised human chromosome 1q cosmids, we have mapped the +P5 fragment to chromosome 1q21-->q22.
authors.affiliation: Centre for Molecular and Cellular Biology, University of Queensland, St. Lucia, Australia.
authors.forename: K
authors.initials: K
authors.lastname: Negus
authors.forename: G H
authors.initials: GH
authors.lastname: Holmes
authors.forename: C
authors.initials: C
authors.lastname: Wicking
authors.forename: B J
authors.initials: BJ
authors.lastname: Wainwright
authors.forename: M H
authors.initials: MH
authors.lastname: Little
mesh_terms: D000818:Animals; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D002874:Chromosome Mapping; D002878:Chromosomes, Human, Pair 1 / Q000235:genetics* / Q000648:ultrastructure; D003360:Cosmids; D004247:DNA / Q000235:genetics* / Q000378:metabolism*; D017931:DNA Primers / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D016162:Genes, Wilms Tumor; D006801:Humans; D006822:Hybrid Cells; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D014157:Transcription Factors / Q000235:genetics*; D025721:WT1 Proteins; D016335:Zinc Fingers / Q000235:genetics*
chemical_list: D017931:DNA Primers; D004268:DNA-Binding Proteins; D014157:Transcription Factors; D025721:WT1 Proteins; D004247:DNA