Effect of UVB radiation on corneal aldehyde dehydrogenase.
OBJECTIVE
A Class 3 aldehyde dehydrogenase happens to be a major soluble protein constituent of the cornea. Its role is conjectured to be manifold: to protect the tissue from oxidative damage by eliminating the toxic aldehydes produced upon lipid peroxidation under oxidative stress, to act as an UV-absorber, and to maintain the level of the coenzyme NADH in the cornea. We have studied the effect of UVB on the structure and enzyme activity of corneal aldehyde dehydrogenase.


METHODS
Aldehyde dehydrogenase was irradiated at 295 nm for varying periods of time and change in its enzyme activity assayed. The structural changes in the molecule accompanying irradiation were monitored using fluorescence and circular dichroism spectroscopy, and its hydrodynamic behavior and surface hydrophobicity studied using gel filtration chromatography and binding of the hydrophobic fluorophore ANS. The protective ability of aldehyde dehydrogenase in preventing aggregation of photolabile proteins, such as Gamma-crystallin of the eye lens, was studied by monitoring the scattering value of the test protein with irradiation by UVB.


RESULTS
Aldehyde dehydrogenase is seen to undergo photodamage with alterations in its quaternary structure, though no significant change is noticed in the peptide chain conformation. Under such conditions the molecule continues to act as a protectant by preventing aggregation of photolabile proteins such as the eye lens Gamma-crystallin.


CONCLUSIONS
Our earlier studies have shown that the free sulfhydryl groups are important for the antioxidant abilities of aldehyde dehydrogenase. Its protective ability towards photoaggregation of Gamma-crystallin seen here might arise both due to: (i) oxyradical quenching and (ii) the increased surface hydrophobicity of the molecule upon irradiation, which allows it to bind to, and thus inhibit the aggregation of interacting proteins.
Uma
L
L
Center for Cellular and Molecular Biology, Uppal Road, Hyderabad 500 007, India.
Jayaram
H
H
Hariharan
J
J
Sharma
Y
Y
Balasubramanian
D
D
D000444:Aldehyde Dehydrogenase / Q000737:chemistry / Q000378:metabolism / Q000528:radiation effects*; D000818:Animals; D002417:Cattle; D003315:Cornea / Q000201:enzymology* / Q000528:radiation effects*; D066298:In Vitro Techniques; D015394:Molecular Structure; D010777:Photochemistry; D011487:Protein Conformation / Q000528:radiation effects; D017433:Protein Structure, Secondary / Q000528:radiation effects; D014466:Ultraviolet Rays / Q000009:adverse effects*
D000444:Aldehyde Dehydrogenase