[The application of immunoblotting micromethod for the detection of oligoclonal Igg in cerebrospinal fluid in multiple sclerosis].
The demonstration of the presence of intrathecal synthesis of immunoglobulins as oligoclonal band (OB) in cerebrospinal fluid (CSF) is considered to be important as an aid to multiple sclerosis diagnosis. We used the Phast System equipment for OB detection in the CSF. Separation was performed on polyacrylamide gels pI 3-9.7. After separation proteins were visualised with silver staining according to manufacturer instruction or transferred electrophoretically on to nitro-cellulose membrane and developed by immunostaining technique. We also calculated indexes of intrathecal synthesis of IgG. We tested CSF of patients with clinically defined multiple sclerosis (n = 29) according to Poser criteria. In all cases IgG indexes were normal. OB were present in 83% with silver staining and in 93% when we used immunoblotting technique.


CONCLUSIONS
1. Detection of OB with Past System is easily performed in routine diagnostic process. 2. Immunoblotting technique increases sensitivity of detection of OB and needs small amounts of CSF. 3. The demonstration of OB in CSF is a method of choice in diagnostic process of inflammatory diseases of nervous system, especially in cases of MS.
Zaborski
J
J
Kliniki Chorób Naczyniowych Układu Nerwowego Instytutu Psychiatrii i Neurologii w Warszawie.
Fiszer
U
U
Korlak
J
J
Członkowska
A
A
D000328:Adult; D005260:Female; D006801:Humans; D015151:Immunoblotting; D007074:Immunoglobulin G / Q000134:cerebrospinal fluid*; D008297:Male; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000134:cerebrospinal fluid*
D007074:Immunoglobulin G
Zastosowanie mikrometody immunoblotingu w wykrywaniu oligoklonalnej immunoglobuliny G w płynie mózgowo-rdzeniowym u chorych na stwardnienie rozsiane.