title: Nucleotide sequences of four pathogen-induced alfalfa peroxidase-encoding cDNAs.
abstract: We constructed an alfalfa cDNA library from mRNA extracted from leaves after infection with Pseudomonas syringae (incompatible interaction). Screening with oligodeoxyribonucleotides designed from regions conserved in all known peroxidases allowed the isolation of four cDNAs (Msprx1A, 1B, 1C and 2). Sequence analysis revealed the presence of open reading frames of 351, 355, 358 and 323 amino acids, respectively, with the characteristic consensus sequences of plant peroxidases. Sequence comparison showed that the Msprx2 product is significantly different from the others and, particularly, lacks a C-terminal propeptide which might be required for vacuolar targeting.
authors.affiliation: Institut des Sciences Végétales, CNRS, Gif-sur-Yvette, France.
authors.forename: J
authors.initials: J
authors.lastname: el-Turk
authors.forename: O
authors.initials: O
authors.lastname: Asemota
authors.forename: J
authors.initials: J
authors.lastname: Leymarie
authors.forename: C
authors.initials: C
authors.lastname: Sallaud
authors.forename: S
authors.initials: S
authors.lastname: Mesnage
authors.forename: C
authors.initials: C
authors.lastname: Breda
authors.forename: D
authors.initials: D
authors.lastname: Buffard
authors.forename: A
authors.initials: A
authors.lastname: Kondorosi
authors.forename: R
authors.initials: R
authors.lastname: Esnault
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D018076:DNA, Complementary; D018744:DNA, Plant; D000455:Medicago sativa / Q000201:enzymology* / Q000382:microbiology; D008969:Molecular Sequence Data; D010544:Peroxidases / Q000145:classification / Q000235:genetics*; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics*; D011549:Pseudomonas / Q000502:physiology*; D017386:Sequence Homology, Amino Acid
chemical_list: D018076:DNA, Complementary; D018744:DNA, Plant; D010940:Plant Proteins; D010544:Peroxidases