Preparation and evaluation of slurry-packed liquid chromatography microcolumns with inner diameters from 12 to 33 microns.
Fused silica capillary liquid chromatography columns with inner diameters between 12 and 33 microns were slurry packed with 5 microns octadecylsilane-modified silica particles. Column efficiencies and van Deemter coefficients were compared. A linear decrease of the A term as column diameter was decreased was the most significant contributor to a lower overall plate height at the optimum velocity.
Hsieh
S
S
Department of Chemistry, University of North Carolina at Chapel Hill, TE 27599-3290, USA.
Jorgenson
J W
JW
D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002853:Chromatography, Liquid / Q000379:methods*; D005069:Evaluation Studies as Topic; D008832:Microchemistry / Q000379:methods; D010316:Particle Size; D012822:Silicon Dioxide
D012822:Silicon Dioxide