[Immunochemical and immunohistochemical study of calpastatin, an endogenous calpain inhibitor, in the masseter muscle of the rabbit].
The calpains-calpastatin system (calcium-activated neutral proteases and endogenous inhibitor) seems to be, in the skeletal muscle, a fine enzymatic system of myofibrillar turnover regulation, in normal as well as pathological conditions (for ex., dystrophic muscle). The purpose of the research is to establish in qualitative and quantitative terms whether the level of calpastatin can evidence differences between a muscle in normal activity conditions and one having dysfunctional alterations experimentally induced. So the masseter muscle of rabbit in normal conditions and with experimentally modified occlusal plane has been used. Our results confirm the presence of the 68 KDa calpastatin in the masseter muscle. The presence of the inhibitor in the same subcellular structures in which the calpains have been detected (myofibrillars, sarcolemma, endomysial connective) has been confirmed. Finally, variations in calpastatin amount in the muscle of animals experimentally treated with respect to the controls have been found. Thus, calpastatin seems to act as a marker of muscle dysfunctions connected to occlusal plane alteration and to loss of vertical dimention.
Pompili
E
E
Istituto di Anatomia Umana, Università Cattolica Sacro Cuore-Roma.
Pantanali
F
F
Deli
R
R
De Renzis
G
G
D000818:Animals; D015153:Blotting, Western; D002135:Calcium-Binding Proteins / Q000276:immunology / Q000493:pharmacokinetics*; D002154:Calpain / Q000276:immunology / Q000493:pharmacokinetics*; D015853:Cysteine Proteinase Inhibitors / Q000276:immunology / Q000493:pharmacokinetics*; D004797:Enzyme-Linked Immunosorbent Assay; D007120:Immunochemistry; D007150:Immunohistochemistry; D008310:Malocclusion / Q000503:physiopathology; D008406:Masseter Muscle / Q000737:chemistry* / Q000276:immunology; D011817:Rabbits; D014072:Tooth Abrasion / Q000503:physiopathology; D014716:Vertical Dimension
D002135:Calcium-Binding Proteins; D015853:Cysteine Proteinase Inhibitors; C033668:calpastatin; D002154:Calpain
Studio immunochimico ed immunoistochimico della calpastatina, inibitore endogeno delle calpaine, nel muscolo massetere di coniglio.