Interferon-gamma-activated macrophages release interleukin-1 alpha to increase tube formation from endothelial cells of rat aorta.
Interferon (IFN)-gamma (160 and 460 pM)-treated peritoneal macrophages (M phi) increase tube formation from subcultured aortic endothelial cells (EC) of rat in type I collagen gel (Kobayashi et al., Immunopharmacology, 27: 23, 1994). The present study was carried out to identify factors released from these M phi. Interleukin (IL)-1 alpha, platelet-derived growth factor (PDGF)-BB and basic fibroblast growth factor (bFGF) increased tube formation, and their 50% effective concentrations were 0.46, 6.4 and 140 pM, respectively. A polyclonal anti-mouse IL-1 alpha antibody (0.33%) inhibited 57.7 +/- 6.5% of the activity of conditioned medium (CM) from IFN-gamma-treated M phi, but not from medium of basal M phi or EC. A monoclonal anti-human bFGF antibody (20 ng/ml) inhibited both the activities of CM from IFN-gamma-treated and basal M phi by 43.2 +/- 15.0% and 46.7 +/- 15.9%, respectively. However, a polyclonal anti-human PDGF-BB antibody (0.33%; 3.3 micrograms/ml) did not inhibit either activity of M phi. These results demonstrate that the effect of IFN-gamma-treated M phi on tube formation was caused by released IL-1 alpha. The release of IL-1 alpha was dependent on the action of IFN-gamma, a different mechanism from those of bFGF and PDGF-BB.
Kobayashi
S
S
Department of Chemical Pharmacology, Toyama Medical and Pharmaceutical University, Japan.
Okabe
M
M
Kimura
I
I
Kimura
M
M
D043925:Angiogenesis Inducing Agents / Q000096:biosynthesis / Q000378:metabolism; D000818:Animals; D001013:Aorta, Thoracic / Q000166:cytology; D002478:Cells, Cultured; D003094:Collagen / Q000378:metabolism; D004730:Endothelium, Vascular / Q000166:cytology / Q000254:growth & development*; D016222:Fibroblast Growth Factor 2 / Q000276:immunology; D007371:Interferon-gamma / Q000276:immunology / Q000494:pharmacology*; D007375:Interleukin-1 / Q000378:metabolism*; D007700:Kinetics; D008262:Macrophage Activation; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D008297:Male; D018919:Neovascularization, Physiologic; D010982:Platelet-Derived Growth Factor / Q000276:immunology; D051381:Rats; D017208:Rats, Wistar
D043925:Angiogenesis Inducing Agents; D007375:Interleukin-1; D010982:Platelet-Derived Growth Factor; D016222:Fibroblast Growth Factor 2; D007371:Interferon-gamma; D003094:Collagen