Identification and quantitation of cholest-5-ene-3 beta,4 beta-diol in rat liver and human plasma.
Cholest-5-ene-3 beta,4 beta-diol (4 beta-hydroxycholesterol) was identified in human plasma and rat liver by gas-liquid chromatography-mass spectrometry. An assay based on isotope dilution-mass spectrometry with a deuterium-labeled internal standard was developed for quantitation of this compound. The concentration of cholest-5-ene-3 beta,4 beta-diol in plasma from healthy subjects was 36 +/- 4.3 ng/ml (mean +/- SD, n = 8). The concentration in rat liver was 0.62 +/- 0.19 microgram/g wet weight (mean +/- SD, n = 6). These levels are of the same order of magnitude as other common oxysterols.
Breuer
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Karolinska Institutet, Department of Medical Laboratory Sciences and Technology, Huddinge University Hospital, Sweden.
D000328:Adult; D000818:Animals; D004032:Diet; D005260:Female; D006801:Humans; D006888:Hydroxycholesterols / Q000032:analysis* / Q000097:blood; D008099:Liver / Q000737:chemistry*; D008297:Male; D008875:Middle Aged; D051381:Rats; D017207:Rats, Sprague-Dawley; D012016:Reference Values
D006888:Hydroxycholesterols; C099828:cholest-5-ene-3,4-diol