Human immune response to HIV-1 Nef. II. Induction of HIV-1/HIV-2 Nef cross-reactive cytotoxic T lymphocytes in peripheral blood lymphocytes of non-infected healthy individuals.
HIV-specific CD8+ cytotoxic T lymphocytes (CTL) are thought to have a beneficial role in HIV infection. In a previous report we have shown that HIV-1 Nef-specific CTL can be readily induced in peripheral blood lymphocytes of seronegative healthy young adults by in vitro stimulation with autologous Epstein-Barr virus-transformed B lymphoblastoid cell lines transfected with the HIV-1 nef gene. Here we demonstrate that these Nef-specific CTL can efficiently lyse HIV-infected primary CD4+ T lymphocytes. CTL of the blood donor tested were Nef-specific and restricted by the autologous MHC class I molecules HLA-A2 and HLA-B7. They recognized HIV-1 Nef in association with both restriction elements but HIV-2 Nef only in association with HLA-B7. The cross-reactivity of the induced effector cells together with the potent immunogenicity of Nef in healthy seronegatives further support the inclusion of Nef as a constituent of HIV vaccines.
Lucchiari-Hartz
M
M
Max-Planck-Institut für Immunbiologie, Freiburg, Germany.
Bauer
M
M
Niedermann
G
G
Maier
B
B
Meyerhans
A
A
Elchmann
K
K
D001402:B-Lymphocytes / Q000821:virology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D016827:CD8 Antigens / Q000276:immunology; D002461:Cell Line, Transformed; D003429:Cross Reactions; D003602:Cytotoxicity, Immunologic; D015693:Gene Products, nef / Q000276:immunology*; D015497:HIV-1 / Q000737:chemistry* / Q000276:immunology*; D015498:HIV-2 / Q000737:chemistry* / Q000276:immunology*; D015789:HLA-A2 Antigen / Q000276:immunology; D015793:HLA-B7 Antigen / Q000276:immunology; D006801:Humans; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014162:Transfection; D054311:nef Gene Products, Human Immunodeficiency Virus
D016827:CD8 Antigens; D015693:Gene Products, nef; D015789:HLA-A2 Antigen; D015793:HLA-B7 Antigen; D054311:nef Gene Products, Human Immunodeficiency Virus