title: Analysis of conserved positions in nuclear RNase P RNA.
abstract: Secondary structure models of eubacterial and eukaryotic nuclear RNase P RNA subunits show extensive structural similarities, allowing the identification of highly conserved nucleotide positions and molecular modeling of the enzyme-substrate complex in three dimensions. Based on this information, we present a preliminary tertiary structure model of the yeast nuclear RNase P RNA. In addition, the most conserved positions in the structure have been subjected to sequence randomization, with viable sequence variations identified by selection in vivo and characterized for phenotypic consequences.
authors.affiliation: Department of Biological Chemistry, University of Michigan, Ann Arbor 40109-0606, USA.
authors.forename: E
authors.initials: E
authors.lastname: Pagán-Ramos
authors.forename: A J
authors.initials: AJ
authors.lastname: Tranguch
authors.forename: J M
authors.initials: JM
authors.lastname: Nolan
authors.forename: N R
authors.initials: NR
authors.lastname: Pace
authors.forename: D R
authors.initials: DR
authors.lastname: Engelke
mesh_terms: D001483:Base Sequence; D002467:Cell Nucleus / Q000201:enzymology; D017124:Conserved Sequence; D004722:Endoribonucleases / Q000737:chemistry / Q000235:genetics*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D016296:Mutagenesis; D009690:Nucleic Acid Conformation; D010641:Phenotype; D016337:RNA, Catalytic / Q000737:chemistry / Q000235:genetics*; D012331:RNA, Fungal / Q000737:chemistry / Q000235:genetics*; D043262:Ribonuclease P; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics
chemical_list: D016337:RNA, Catalytic; D012331:RNA, Fungal; D004722:Endoribonucleases; D043262:Ribonuclease P