A multicopy suppressor gene, MSS10, restores STA2 expression in Saccharomyces cerevisiae strains containing the STA10 repressor gene.
Transcription of the three unlinked, homologous STA1-3 glucoamylase-encoding genes, involved in starch degradation by Saccharomyces cerevisiae, was previously shown to be down-regulated by the presence of STA10, acting via three upstream repression sequence regions that were identified in the STA2 promoter. Here we report the cloning and characterization of a putative transcriptional activator gene, MSS10 (multicopy suppressor of STA10), which, when present in multiple copies, overcomes STA10 repression. Deletion of MSS10, located on chromosome XV, resulted in media-specific extinction of glucoamylase synthesis. The nucleotide sequence of MSS10 is identical to three other genes from S. cerevisiae identified as: FUP1, a gene that enhances iron-limited growth; PHD2, a gene identified for its ability to induce pseudohyphal growth in diploid cells grown on nitrogen-limited media; and MSN1, a gene encoding a transcriptional activator involved in invertase regulation.
Lambrechts
M G
MG
Department of Microbiology and Institute for Wine Biotechnology, University of Stellenbosch, Stellenbosch 7600, South Africa.
Sollitti
P
P
Marmur
J
J
Pretorius
I S
IS
D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000235:genetics; D004171:Diploidy; D015870:Gene Expression; D005800:Genes, Fungal; D005809:Genes, Regulator; D016153:Genes, Suppressor; D005087:Glucan 1,4-alpha-Glucosidase / Q000096:biosynthesis / Q000235:genetics; D006579:Heterozygote; D005810:Multigene Family; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D013213:Starch / Q000378:metabolism; D015533:Transcriptional Activation
D004271:DNA, Fungal; D013213:Starch; D005087:Glucan 1,4-alpha-Glucosidase