title: An E-selectin-IgG chimera supports sialylated moiety dependent adhesion of colon carcinoma cells under fluid flow.
abstract: To further characterize the molecular mechanisms that govern carcinoma cell adhesion to stimulated endothelium, we studied the adhesion of a human colon carcinoma cell line, KM12-L4, to an E-selectin-IgG1 chimera and interleukin (IL)-1 beta-stimulated human umbilical vein endothelial cells (HUVEC) under in vitro fluid flow conditions. Between 0.6 and 1.8 dynes/cm2, KM12-L4 cells attach to and roll on IL-1 beta-stimulated HUVEC. The adhesion is E-selectin dependent and diminished upon pretreatment of the KM12-L4 cells with neuraminidase (neuraminidase sensitive). Between 0.7 and 1.8 dynes/cm2, surfaces coated with an E-selectin-IgG1 chimera support attachment and rolling of KM12-L4 cells. The adhesion to the E-selectin-IgG1 chimera is blocked by an antibody to the lectin domain of E-selectin and is neuraminidase sensitive. Rolling KM12-L4 cells exhibit variable velocity motion over both IL-1 beta-stimulated HUVEC and E-selectin-IgG1 chimera-coated surfaces. Our results provide the first direct evidence that sialylated moieties are involved in the adhesion of carcinoma cells to IL-1 beta-stimulated endothelium under flow conditions; E-selectin-IgG1 chimeras can support cell attachment and rolling under defined flow conditions; the topology of the endothelium is not the sole cause of variable velocity motion observed in cell rolling systems.
authors.affiliation: School of Chemical Engineering, Cornell University, Ithaca, NY 14853, USA.
authors.forename: D J
authors.initials: DJ
authors.lastname: Goetz
authors.forename: B K
authors.initials: BK
authors.lastname: Brandley
authors.forename: D A
authors.initials: DA
authors.lastname: Hammer
mesh_terms: D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology*; D003110:Colonic Neoplasms / Q000503:physiopathology*; D019040:E-Selectin / Q000502:physiology; D004730:Endothelium, Vascular / Q000502:physiology*; D005434:Flow Cytometry; D006801:Humans; D007074:Immunoglobulin G / Q000494:pharmacology / Q000502:physiology; D007375:Interleukin-1 / Q000494:pharmacology / Q000502:physiology; D009439:Neuraminidase / Q000378:metabolism / Q000494:pharmacology; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012212:Rheology; D013499:Surface Properties; D014407:Tumor Cells, Cultured / Q000502:physiology; D014471:Umbilical Veins / Q000502:physiology
chemical_list: D019040:E-Selectin; D007074:Immunoglobulin G; D007375:Interleukin-1; D011993:Recombinant Fusion Proteins; D009439:Neuraminidase