Complete nucleotide sequence of the mouse CTLA8 gene.
The gene encoding the mouse cytotoxic T-lymphocyte-associated antigen 8 (CTLA8) has been cloned and its complete nucleotide (nt) sequence determined. Sequence and polymerase chain reaction (PCR) analysis indicated that the published CTLA8 sequence[Rouvier et al., J. Immunol. 150 (1993), 5445-5456] was of rat rather than mouse origin. The mouse CTLA8 gene contains two exons and one intron. The 5'flanking region contains several consensus motifs for binding to transcription factors and the 3' untranslated region (UTR) has AU-rich motifs associated with RNA instability. The putative exon sequences predict that the full-length mouse CTLA8 molecule contains 147 amino acids (aa) and shares 88% aa identity with rat CTLA8 and 57% aa identity to HSV13, an open reading frame (ORF) from herpesvirus saimiri (HVS).
Yao
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Z
Immunex Corporation, Seattle, Washington 98101, USA. yaoz@immunex.com
Timour
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M
Painter
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S
Fanslow
W
W
Spriggs
M
M
D000818:Animals; D000945:Antigens, Differentiation, T-Lymphocyte / Q000096:biosynthesis / Q000235:genetics*; D001483:Base Sequence; D001665:Binding Sites; D003001:Cloning, Molecular; D016384:Consensus Sequence; D017124:Conserved Sequence; D017931:DNA Primers; D004268:DNA-Binding Proteins / Q000378:metabolism; D015698:Genomic Library; D006567:Herpesvirus 2, Saimiriine / Q000235:genetics; D020381:Interleukin-17; D051379:Mice / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D016133:Polymerase Chain Reaction; D051381:Rats; D011994:Recombinant Proteins / Q000096:biosynthesis; D012689:Sequence Homology, Nucleic Acid; D013601:T-Lymphocytes / Q000276:immunology; D016385:TATA Box
D000945:Antigens, Differentiation, T-Lymphocyte; D017931:DNA Primers; D004268:DNA-Binding Proteins; C098680:Il17a protein, mouse; D020381:Interleukin-17; D011994:Recombinant Proteins