[Recombinant proteins containing oxytocin oligomeric sequences].
Expression plasmids were constructed with genes encoding the ILOX3, ILOX6, and ILOX9 recombinant proteins, which contain the C-terminal fragments of trimer, hexamer, or nonamer of oxytocinoyl-Lys. Upon expression in E. coli, all three genes yielded inclusion bodies containing protein products of similar length and heterogeneous in the C-terminal region. It is likely that in the case of the ilox3 gene, the obtained protein mixture includes the full-length product of translation with the C-terminal lysine. In the case of the ilox6 and ilox9 genes, the protein products are formed as the result of a site-specific proteolysis in the regions between the second and the fourth oxytocin units.
Gurevich
A I
AI
Kachalina
T A
TA
Kaiushin
A L
AL
Korosteleva
M D
MD
Mal'tsev
K V
KV
Mirgorodskaia
O A
OA
Miroshnikov
A I
AI
D000595:Amino Acid Sequence; D001483:Base Sequence; D001704:Biopolymers; D004274:DNA, Recombinant; D006868:Hydrolysis; D008969:Molecular Sequence Data; D010121:Oxytocin / Q000235:genetics* / Q000378:metabolism; D010957:Plasmids; D014176:Protein Biosynthesis; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
D001704:Biopolymers; D004274:DNA, Recombinant; D011994:Recombinant Proteins; D010121:Oxytocin
Rekombinantnye belki, soderzhashchie oligomernye posledovatel'nosti oksitotsina.