Specific deposition of passively transferred monoclonal antibodies against herpes simplex virus type 1 in rat brain infected with the virus.
The kinetics of human monoclonal antibody (anti-gB) to herpes simplex virus type 1 (HSV-1) were investigated after intravenous injection of anti-gB into an HSV-1 encephalitis animal model. Immunohistochemical study revealed specific deposition of passively transferred anti-gB in the hippocampus and thalamus of the infected rat brain, and it bound to the same neurons in which HSV-1 antigen was positively stained. To examine the macroscopic distribution of anti-gB in the infected brain, we undertook an 125I-labeled anti-gB injection study, and the same distribution of 125I-labeled anti-gB deposition was observed by brain semimicroautoradiography as in the immunohistochemical study. These results suggest that anti-gB easily permeates the capillary wall and is deposited in the inflammatory site where HSV-1-specific antigen is detectable. The use of radioisotope-labeled anti-gB injection and external brain imaging could lead to a noninvasive diagnostic tool for the early detection of HSV-1 antigen in cases of suspected HSV-1 encephalitis.
Tamada
K
K
Division of Pediatrics, Tachikawa Kyosai Hospital, Tokyo, Japan.
Fujinaga
S
S
Watanabe
R
R
Yamashita
R
R
Takeuchi
Y
Y
Osano
M
M
D000818:Animals; D000911:Antibodies, Monoclonal / Q000493:pharmacokinetics*; D001921:Brain / Q000382:microbiology; D018792:Encephalitis, Viral / Q000276:immunology* / Q000382:microbiology; D005260:Female; D006561:Herpes Simplex / Q000276:immunology* / Q000821:virology; D018259:Herpesvirus 1, Human / Q000276:immunology*; D007116:Immunization, Passive; D007150:Immunohistochemistry; D007457:Iodine Radioisotopes; D051381:Rats; D014759:Viral Envelope Proteins / Q000276:immunology* / Q000302:isolation & purification; D014779:Virus Replication
D000911:Antibodies, Monoclonal; D007457:Iodine Radioisotopes; D014759:Viral Envelope Proteins; C040495:glycoprotein B, Simplexvirus