Mode of antibacterial action of totarol, a diterpene from Podocarpus nagi.
The antimicrobial mechanism of totarol was studied using Pseudomonas aeruginosa IFO 3080. This diterpene inhibited oxygen consumption and respiratory-driven proton translocation in whole cells, and oxidation of NADH in membrane preparation. NADH-cytochrome c reductase was inhibited by totarol while cytochrome c oxidase was not. NADH-DPIP reductase and NADH-CoQ reductase were also inhibited. The site of respiratory inhibition of totarol was thought to be near CoQ in the bacterial electron transport chain.
Haraguchi
H
H
Faculty of Engineering, Fukuyama University, Japan.
Oike
S
S
Muroi
H
H
Kubo
I
I
D045784:Abietanes; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002462:Cell Membrane / Q000378:metabolism; D004224:Diterpenes / Q000494:pharmacology*; D004579:Electron Transport / Q000187:drug effects; D042967:Electron Transport Complex I; D003576:Electron Transport Complex IV / Q000378:metabolism; D004926:Escherichia coli / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D009243:NAD / Q000378:metabolism; D009245:NADH Dehydrogenase / Q000037:antagonists & inhibitors; D009247:NADH, NADPH Oxidoreductases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D010101:Oxygen Consumption / Q000187:drug effects; D010936:Plant Extracts; D018517:Plant Roots; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000378:metabolism; D013211:Staphylococcus aureus / Q000187:drug effects
D045784:Abietanes; D000900:Anti-Bacterial Agents; D004224:Diterpenes; D010936:Plant Extracts; D009243:NAD; C078548:totarol; D009247:NADH, NADPH Oxidoreductases; D009245:NADH Dehydrogenase; D003576:Electron Transport Complex IV; D042967:Electron Transport Complex I