title: Screening for IgA deficiency on the Olympus PK7100 by haemagglutination inhibition.
abstract: Severe or life-threatening anaphylactic reactions during blood transfusion to patients with anti-IgA, though infrequent, can be avoided by transfusing blood products prepared from IgA deficient donors. This report describes the development and use a passive haemagglutination assay adapted for use on the Olympus PK7100 blood group analyser. Donor plasma samples identified as being presumptive IgA deficient were further screened by a manual passive haemagglutination inhibition technique and finally confirmed by means of a quantitative enzyme-linked immunosorbent assay. Of 5723 donors tested simultaneously for IgA deficiency, blood grouping and red cell antibody screening, six (1 in 954) were confirmed as IgA deficient (i.e. < 100 ng/ml of IgA). The test is simple and cost effective and can be used to establish a database of IgA-deficient donors.
authors.affiliation: National Blood Transfusion Service (Wales), Rhydlafar, Nr St Fagans, Cardiff, UK.
authors.forename: L A
authors.initials: LA
authors.lastname: Mohabir
authors.forename: T J
authors.initials: TJ
authors.lastname: Rees
mesh_terms: D001788:Blood Grouping and Crossmatching / Q000295:instrumentation*; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D017098:IgA Deficiency / Q000175:diagnosis*; D008403:Mass Screening / Q000379:methods*