Construction of a cytogenetically characterized porcine somatic cell hybrid panel and its use as a mapping tool.
A new panel of cytogenetically characterized pig-rodent somatic cell hybrids was constructed and tested for twelve microsatellite markers with PCR. Cytogenetic characterization of hybrids was accomplished by fluorescence painting and GTG-banding of metaphase chromosomes. The panel consists of 15 independent pig-hamster and 6 independent pig-mouse cell lines. In the panel, all pig autosomes and the X Chromosome (Chr) are represented, and it is informative for all chromosome pairs except 2-14, 2-15, 3-9, 14-15, 14-16, and 16-17. The microsatellites tested were S0022, S0023, S0084, S0098, S0112, S0113, S0114, S0115, S0117, S0118, S0119, and S0120. The PCR results obtained in the 21 hybrids were compared with the cytogenetic data and analyzed for concordancy and correlation. Eight microsatellites could be assigned to specific pig chromosomes, confirming seven assignments based on linkage analysis.
Zijlstra
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Department of Cell Biology and Histology, Faculty of Veterinary Medicine, Utrecht University, PO Box 80.157, 3508 TD Utrecht, The Netherlands.
Bosma
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AA
de Haan
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NA
Mellink
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C
D000818:Animals; D002874:Chromosome Mapping / Q000379:methods*; D006224:Cricetinae; D003582:Cytogenetics; D008040:Genetic Linkage; D006822:Hybrid Cells; D051379:Mice; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction; D013552:Swine / Q000235:genetics*