Ameliorating effect of vitamin C on murine sperm toxicity induced by three pesticides (endosulfan, phosphamidon and mancozeb).
The ameliorating effect of vitamin C (injected intraperitoneally) was evaluated against changes in sperm count and sperm head morphology in mice fed either 3, 6 or 1000 mg/kg body wt/day endosulfan, phosphamidon or mancozeb, respectively. The animals received aqueous preparations of the pesticides and/or vitamin C once daily for 35 consecutive days. All three pesticides, irrespective of their chemical nature, significantly decreased the sperm count, as well as increased the frequency of sperm with aberrant head morphology. Out of the three doses of vitamin C used the middle and higher ones (20 and 40 mg/kg body wt/day, respectively) afforded comparatively more significant amelioration. The lower dose (10 mg/kg body wt/day) of this vitamin (quantitatively equivalent to the human therapeutic dose according to body weight) was least efficacious in both the tests. However, amelioration was never up to the control level in any case. Vitamin C doses, when administered alone, did not produce any adverse effect on sperm count and sperm head morphology.
Khan
P K
PK
Department of Zoology, Bhagalpur University, India.
Sinha
S P
SP
D000818:Animals; D016587:Antimutagenic Agents / Q000008:administration & dosage / Q000494:pharmacology; D001205:Ascorbic Acid / Q000008:administration & dosage / Q000494:pharmacology*; D004347:Drug Interactions; D004726:Endosulfan / Q000633:toxicity; D005659:Fungicides, Industrial / Q000633:toxicity; D006801:Humans; D007306:Insecticides / Q000633:toxicity; D008297:Male; D008344:Maneb / Q000633:toxicity; D051379:Mice; D008815:Mice, Inbred Strains; D009153:Mutagens / Q000633:toxicity*; D010575:Pesticides / Q000633:toxicity*; D010707:Phosphamidon / Q000633:toxicity; D013076:Sperm Count / Q000187:drug effects; D013077:Sperm Head / Q000187:drug effects / Q000473:pathology; D013094:Spermatozoa / Q000187:drug effects*; D015038:Zineb / Q000633:toxicity
D016587:Antimutagenic Agents; D005659:Fungicides, Industrial; D007306:Insecticides; D009153:Mutagens; D010575:Pesticides; D008344:Maneb; D010707:Phosphamidon; D004726:Endosulfan; D001205:Ascorbic Acid; C013099:mancozeb; D015038:Zineb