Arrested DNA replication in Xenopus and release by Escherichia coli mutagenesis proteins.
Xenopus oocytes and oocyte nuclear extracts repair ultraviolet photoproducts on double-stranded (ds) DNA and replicate single-stranded (ss) to ds DNA. M13 ss DNA molecules containing cyclobutane pyrimidine dimers were maintained but not replicated in Xenopus oocytes yet were replicated in progesterone-matured oocytes. The replication arrest functioned only in cis. The replication arrest was alleviated by injection into oocytes of messenger RNAs encoding the prokaryotic mutagenesis proteins UmuD'C or MucA'B. These results may help explain how cells stabilize repair or replication events on DNA with unrepairable lesions.
Oda
N
N
Office of Scientific Director, National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK), National Institutes of Health, Bethesda, Maryland 20892, USA.
Levin
J D
JD
Spoonde
A Y
AY
Frank
E G
EG
Levine
A S
AS
Woodgate
R
R
Ackerman
E J
EJ
D000818:Animals; D001426:Bacterial Proteins / Q000502:physiology*; D017104:Bacteriophage M13 / Q000235:genetics; D010584:Bacteriophage phi X 174 / Q000235:genetics; D004247:DNA / Q000096:biosynthesis; D004249:DNA Damage; D004260:DNA Repair; D004261:DNA Replication; D004277:DNA, Single-Stranded / Q000096:biosynthesis; D004259:DNA-Directed DNA Polymerase; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins; D009865:Oocytes / Q000378:metabolism*; D014466:Ultraviolet Rays; D014981:Xenopus
D001426:Bacterial Proteins; D004277:DNA, Single-Stranded; D029968:Escherichia coli Proteins; C063757:MucA protein, Bacteria; C093590:MucB protein, Bacteria; D004247:DNA; C078202:UmuC protein, E coli; D004259:DNA-Directed DNA Polymerase; C055218:UmuD protein, E coli