Substitution of L-fucose by L-galactose in cell walls of Arabidopsis mur1.
An Arabidopsis thaliana mutant (mur1) has less than 2 percent of the normal amounts of L-fucose in the primary cell walls of aerial portions of the plant. The survival of mur1 plants challenged the hypothesis that fucose is a required component of biologically active oligosaccharides derived from cell wall xyloglucan. However, the replacement of L-fucose (that is, 6-deoxy-L-galactose) by L-galactose does not detectably alter the biological activity of the oligosaccharides derived from xyloglucan. Thus, essential structural and conformational features of xyloglucan and xyloglucan-derived oligosaccharides are retained when L-galactose replaces L-fucose.
Zablackis
E
E
Complex Carbohydrate Research Center, University of Georgia, Athens 30602-4712, USA.
York
W S
WS
Pauly
M
M
Hantus
S
S
Reiter
W D
WD
Chapple
C C
CC
Albersheim
P
P
Darvill
A
A
D015084:2,4-Dichlorophenoxyacetic Acid / Q000494:pharmacology; D017360:Arabidopsis / Q000737:chemistry* / Q000235:genetics / Q000502:physiology; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002473:Cell Wall / Q000737:chemistry* / Q000502:physiology; D005643:Fucose / Q000032:analysis* / Q000502:physiology; D005647:Fucosyltransferases / Q000378:metabolism; D005690:Galactose / Q000032:analysis* / Q000502:physiology; D005936:Glucans; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009154:Mutation; D009844:Oligosaccharides / Q000494:pharmacology; D018532:Pisum sativum; D018547:Plant Stems / Q000187:drug effects / Q000254:growth & development; D011134:Polysaccharides / Q000737:chemistry*; D016339:Spectrometry, Mass, Fast Atom Bombardment; D014990:Xylans
D005936:Glucans; D009844:Oligosaccharides; D011134:Polysaccharides; D014990:Xylans; D015084:2,4-Dichlorophenoxyacetic Acid; D005643:Fucose; C029353:xyloglucan; D005647:Fucosyltransferases; D005690:Galactose