title: The lytA gene and the DNA region located downstream of this gene are not involved in the formation of the type 3 capsular polysaccharide of Streptococcus pneumoniae.
abstract: Streptococcus pneumoniae strain M31, an unencapsulated, serotype 2 (S2(-)) mutant having a deletion of at least 5.5 kb containing the gene lytA that encodes the main pneumococcal autolysin, was transformed to the encapsulated serotype 3 (S3(+)) with DNA from the clinical pneumococcal strain 406. Hybridization analysis revealed that the S3(+) transformants also have the deletion demonstrating that lytA and the DNA region located downstream of this gene are not involved in the encapsulation of S. pneumoniae.
authors.affiliation: Departamento de Microbiología Molecular, Centro de Investigaciones Biológicas, CSIC, 28006 Madrid, Spain.
authors.forename: E
authors.initials: E
authors.lastname: García
authors.forename: C
authors.initials: C
authors.lastname: Arrecubieta
authors.forename: R
authors.initials: R
authors.lastname: López
mesh_terms: D016667:Bacterial Capsules / Q000096:biosynthesis* / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics*; D017353:Gene Deletion; D005798:Genes, Bacterial / Q000502:physiology*; D009238:N-Acetylmuramoyl-L-alanine Amidase / Q000235:genetics*; D013296:Streptococcus pneumoniae / Q000201:enzymology / Q000235:genetics*; D014169:Transformation, Bacterial
chemical_list: D004269:DNA, Bacterial; D009238:N-Acetylmuramoyl-L-alanine Amidase