[Detection of tyrosinase mRNA using reverse transcription/polymerase chain reaction with fine needle punctures of melanoma metastases].
Tyrosinase mRNA produced by melanoma cells can be detected by reverse transcriptase-polymerase chain reaction (rt-pcr) with fine-needle tissue punctures. Fine-needle punctures from six suspected skin and lymph node metastases in three patients with malignant melanoma were analysed via rt-pcr for tyrosinase mRNA. All the suspected metastases were surgically removed and histologically examined separately. In each case the rt-pcr results and the histological diagnosis corresponded. This less invasive and highly sensitive method could prove to be a useful alternative in the diagnosis of melanoma metastasis.
Hofmann
S
S
Dermatologische Klinik und Poliklinik der TU, München.
Remy
W
W
Borelli
S
S
von Reis
A
A
Weidinger
S
S
D000368:Aged; D000369:Aged, 80 and over; D001707:Biopsy, Needle; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D006801:Humans; D008198:Lymph Nodes / Q000473:pathology; D008297:Male; D008545:Melanoma / Q000235:genetics / Q000473:pathology*; D008875:Middle Aged; D014442:Monophenol Monooxygenase / Q000235:genetics*; D009362:Neoplasm Metastasis; D009367:Neoplasm Staging; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics*; D012867:Skin / Q000473:pathology; D012878:Skin Neoplasms / Q000235:genetics / Q000473:pathology*; D014158:Transcription, Genetic / Q000235:genetics*
D012333:RNA, Messenger; D014442:Monophenol Monooxygenase
Nachwesi von Tyrosinase-mRNA mittels Reverser Transkription/Polymerasekettenreaktion an Feinnadelpunktaten von Melanommetastasen.