Early pathologic and biochemical changes in Creutzfeldt-Jakob disease: study of brain biopsies.
We examined brain biopsy tissue from five patients with a neurologic syndrome consistent with Creutzfeldt-Jakob disease using Western blot analysis and immunohistochemistry for the detection of protease-resistant prion protein, in addition to histopathologic examination. Our results indicate that the formation of protease-resistant prion protein is an early event in disease pathogenesis and Western blot analysis can detect protease-resistant prion protein in the absence of structural lesions using a small amount of brain biopsy tissue.
Castellani
R
R
Institute of Pathology, Division of Neuropathology, Cleveland, OH 44106, USA.
Parchi
P
P
Stahl
J
J
Capellari
S
S
Cohen
M
M
Gambetti
P
P
D000328:Adult; D000368:Aged; D001706:Biopsy; D015153:Blotting, Western; D001921:Brain / Q000473:pathology*; D001923:Brain Chemistry; D007562:Creutzfeldt-Jakob Syndrome / Q000175:diagnosis / Q000378:metabolism / Q000473:pathology*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D011328:Prions / Q000032:analysis*
D011328:Prions