Mapping of four mouse genes encoding eye lens-specific structural, gap junction, and integral membrane proteins: Cryba1 (crystallin beta A3/A1), Crybb2 (crystallin beta B2), Gja8 (MP70), and Lim2 (MP19).
Four genes encoding eye lens-specific proteins, potential candidate genes for congenital cataract (CC) mutations, were mapped in the mouse genome using a panel of somatic cell hybrids and DNAs from the EU-CIB (European Collaborative Interspecific Backcross). Two of them are lens fiber cell structural proteins: the Cryba1 locus encoding crystallinbetaA3/A1 maps to chromosome 11, 2.5 +/- 2.5 cM distal to D11Mit31, and the Crybb2 locus encoding crystallinbetaB2 maps to chromosome 5, 9.1 +/- 4.3 cM distal to D5Mit88. The other two genes encode lens-specific gap junction and integral membrane proteins, respectively: The Gja8 locus encoding gap juction membrane channel protein alpha8, also called connexin50 or MP70, maps to chromosome 3, 11.9 +/- 5.0 cM distal to D3Mit22, and the Lim2 locus encoding lens intrinsic membrane protein 2, also called MP19, maps to chromosome 7, 2.5 +/- 2.5 cM proximal to Ngfg. All four map positions, when compared with the corresponding positions in human, lie within known regions of conserved synteny between mouse and human chromosomes.
Kerscher
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MRC Radiobiology Unit, Didcot, Oxfordshire, United Kingdom.
Church
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Boyd
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Lyon
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D000818:Animals; D002874:Chromosome Mapping; D017630:Connexins; D006224:Cricetinae; D003412:Cricetulus; D003433:Crosses, Genetic; D003459:Crystallins / Q000235:genetics*; D005136:Eye Proteins / Q000235:genetics*; D005260:Female; D016678:Genome; D006822:Hybrid Cells; D007908:Lens, Crystalline / Q000378:metabolism*; D008297:Male; D008562:Membrane Glycoproteins; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D011995:Recombination, Genetic; D015183:Restriction Mapping; D038205:beta-Crystallin A Chain; D000099264:Connexin 50
D017630:Connexins; D003459:Crystallins; D005136:Eye Proteins; D008562:Membrane Glycoproteins; D038205:beta-Crystallin A Chain; D000099264:Connexin 50; C489108:CRYBA1 protein, human; C051374:Cryba1 protein, mouse; C489025:Lim2 protein, mouse