Sequence, genomic structure, and chromosomal assignment of human DOC-2.
DOC-2 is a human gene originally identified as a 767-bp cDNA fragment isolated from normal ovarian epithelial cells by differential display against ovarian carcinoma cells. We have now determined the complete cDNA sequence of the 3.2-kb DOC-2 transcript and localized the gene to chromosome 5. A 12.5-kb genomic fragment at the 5'-end of DOC-2 has also been sequenced, revealing the intron-exon structure of the first eight exons (788 bases) of the DOC-2 gene. Translation of the DOC-2 cDNA predicts a hydrophobic protein of 770 amino acid residues with a molecular weight of 82.5 kDa. Comparison of the DNA and amino acid sequences of DOC-2 to publicly accessible sequence databases revealed 83% identify to p96, a murine protein of similar size, thought to be a mitogen-responsive phosphoprotein. In addition, about 45% identity was observed between the first 140 N-terminal residues of DOC-2 and the Caenorhabditas elegans M110.5 and Drosophila melanogaster Dab genes.
Albertsen
H M
HM
Eccles Institute of Human Genetics, Salt Lake City, Utah, USA. Hans.Albertsen@genetics.utah.edu
Smith
S A
SA
Melis
R
R
Williams
B
B
Holik
P
P
Stevens
J
J
White
R
R
D048868:Adaptor Proteins, Signal Transducing; D033942:Adaptor Proteins, Vesicular Transport; D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D051017:Apoptosis Regulatory Proteins; D001483:Base Sequence; D002460:Cell Line; D002895:Chromosomes, Human, Pair 5; D018076:DNA, Complementary; D005091:Exons; D005260:Female; D016147:Genes, Tumor Suppressor; D006801:Humans; D007438:Introns; D008969:Molecular Sequence Data; D011506:Proteins / Q000235:genetics*; D012377:Rodentia; D017386:Sequence Homology, Amino Acid; D025521:Tumor Suppressor Proteins
D048868:Adaptor Proteins, Signal Transducing; D033942:Adaptor Proteins, Vesicular Transport; D051017:Apoptosis Regulatory Proteins; C102885:DAB2 protein, human; D018076:DNA, Complementary; D011506:Proteins; D025521:Tumor Suppressor Proteins