title: Rabbit liver phosphofructokinase: rapid purification and phosphorylation site identification.
abstract: Liver phosphofructokinase can be selectively precipitated by the addition of protamine sulfate to a heat-treated crude extract and redissolved, giving nearly full recovery of catalytic activity in combination with a 67-fold increase in specific activity. We have incorporated protamine sulfate precipitation into a five step purification procedure that can be completed in one day--giving 47% recovery of electrophoretically homogeneous liver phosphofructokinase having a specific activity of 50 units/mg. The radio-labeled fragment isolated from a CNBr digest of liver phosphofructokinase that has undergone in vitro phosphorylation catalyzed by the cAMP-dependent protein kinase has the sequence AEYVSGELEHVTRRSLS.
authors.affiliation: Department of Biochemistry and Biophysics, Oregon State University, Corvallis 97331, USA.
authors.forename: Z
authors.initials: Z
authors.lastname: Zhao
authors.forename: R W
authors.initials: RW
authors.lastname: Pascalar
authors.forename: D A
authors.initials: DA
authors.lastname: Malencik
authors.forename: S R
authors.initials: SR
authors.lastname: Anderson
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000201:enzymology; D002850:Chromatography, Gel; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D051379:Mice; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000201:enzymology; D010446:Peptide Fragments / Q000737:chemistry / Q000302:isolation & purification; D010732:Phosphofructokinase-1 / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism; D010748:Phosphopeptides / Q000737:chemistry / Q000302:isolation & purification; D010766:Phosphorylation; D011479:Protamines; D011817:Rabbits; D017386:Sequence Homology, Amino Acid
chemical_list: D010446:Peptide Fragments; D010748:Phosphopeptides; D011479:Protamines; D000255:Adenosine Triphosphate; D010732:Phosphofructokinase-1