title: Carbohydrate-deficient transferrin evaluation in dry blood spots.
abstract: The aim of this study was to assess the performance of the isoelectric focusing/immunoblotting/laser densitometry (IEF/IB/LD) procedure to evaluate carbohydrate-deficient transferrin (CDT) derived from dry blood spots. Serum specimens obtained from insurance applicants were analyzed for CDT by IEF/IB/LD. Dry blood spots derived from 50 serum specimens were analyzed by IEF/IB/LD. A comparative analysis of these serum specimens and the paired dry blood spots by IEF/IB/LD shows a highly significant correlation of the CDT values (r = 0.94, p < 0.0001). Stability studies indicate that, under proper storage conditions (2-3 days at room temperature, 2 weeks at 4 degrees C, or frozen at or below -20 degrees C indefinitely), dry blood spots can be used as a source of CDT for analysis by IEF/IB/LD, thus simplifying sampling, storage, and transportation of specimens to the testing site.
authors.affiliation: Specialty Laboratories, Inc., Santa Monica, California, USA.
authors.forename: P
authors.initials: P
authors.lastname: Bean
authors.forename: M S
authors.initials: MS
authors.lastname: Sutphin
authors.forename: P
authors.initials: P
authors.lastname: Necessary
authors.forename: M S
authors.initials: MS
authors.lastname: Agopian
authors.forename: K
authors.initials: K
authors.lastname: Liegmann
authors.forename: C
authors.initials: C
authors.lastname: Ludvigsen
authors.forename: J B
authors.initials: JB
authors.lastname: Peter
mesh_terms: D000437:Alcoholism / Q000097:blood / Q000175:diagnosis*; D001801:Blood Stains; D003720:Densitometry; D006801:Humans; D015151:Immunoblotting; D007341:Insurance; D007525:Isoelectric Focusing; D014168:Transferrin / Q000031:analogs & derivatives* / Q000032:analysis
chemical_list: D014168:Transferrin; C056759:carbohydrate-deficient transferrin