Molecular and genetic studies on the region of translocation and duplication in the neuroblastoma cell line NGP at the 1p36.13-p36.32 chromosomal site.
Cytogenetic and molecular studies suggest that chromosome 1p might contain oncosuppressor genes involved in the pathogenesis of neuroblastoma and other adult tumors. The isolation of these genes by the 'positional cloning' approach will be facilitated by the characterization of cell lines with well defined chromosomal aberrations. In the present report we provide molecular data on the NGP neuroblastoma cell line which contains a reciprocal t(1;15) translocation. Two regions, possibly hosting oncosuppressor genes, have been identified: one is distal to the ENO1 locus, the other one is comprised between PND and A12M2 and corresponds to that of a constitutional t(1;17) translocation described in a neuroblastoma patient. Genetic data also suggest that the NGP cell line, despite the presence of two chromosomes 1, might be hemizygous for the subtelomeric 1p region.
Casciano
I
I
Laboratory of Population Genetics, Istituto Nazionale per la Ricerca sul Cancro, Alghero, Italy.
Marchi
J V
JV
Muresu
R
R
Volpi
E V
EV
Rozzo
C
C
Opdenakker
G
G
Romani
M
M
D000328:Adult; D001483:Base Sequence; D018244:Chromosomes, Artificial, Yeast / Q000235:genetics; D002878:Chromosomes, Human, Pair 1; D002884:Chromosomes, Human, Pair 15; D004273:DNA, Neoplasm / Q000235:genetics; D015321:Gene Rearrangement; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D005810:Multigene Family; D009447:Neuroblastoma / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid; D014178:Translocation, Genetic; D014407:Tumor Cells, Cultured
D004273:DNA, Neoplasm