Sequence tags of provirus integration sites in DNAs of tumors induced by the murine retrovirus SL3-3.
The murine retrovirus SL3-3 is a potent inducer of T-cell lymphomas when inoculated into susceptible newborn mice. The proviral integration site sequences were surveyed in tumor DNAs by a simple two-step PCR method. From 20 SL3-3-induced tumors a total of 39 provirus-host junctions were amplified and sequenced. Seven showed homology to known sequences. These included the known common integration site c-myc as well as genes not previously identified as targets of provirus integration, namely N-ras and the genes coding for major histocompatibility complex class 11 E-beta, protein kinase C-eta, and T-cell receptor beta-chain. Among these genes, the integrations in c-myc as well as the one in N-ras were found to be clonal. One of the remaining 32 proviral integration site sequences that show no similarities to known sequences may represent a common integration site, as 2 of the 20 tumors demonstrated clonal provirus insertion into this region.
Sørensen
A B
AB
Department of Molecular and Structural Biology, University of Aarhus, Denmark.
Duch
M
M
Amtoft
H W
HW
Jørgensen
P
P
Pedersen
F S
FS
D000818:Animals; D001483:Base Sequence; D004273:DNA, Neoplasm / Q000235:genetics*; D005802:Genes, MHC Class II; D016259:Genes, myc; D011905:Genes, ras; D009052:Leukemia Virus, Murine / Q000235:genetics*; D016399:Lymphoma, T-Cell / Q000235:genetics / Q000821:virology*; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011493:Protein Kinase C / Q000235:genetics; D017346:Protein Serine-Threonine Kinases; D011518:Proto-Oncogene Proteins / Q000235:genetics; D051573:Proto-Oncogene Proteins c-pim-1; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid; D016662:Virus Integration
D004273:DNA, Neoplasm; D011518:Proto-Oncogene Proteins; D016693:Receptors, Antigen, T-Cell, alpha-beta; C496636:Pim1 protein, mouse; D017346:Protein Serine-Threonine Kinases; D051573:Proto-Oncogene Proteins c-pim-1; D011493:Protein Kinase C