Ionic conductivity, transference numbers, composition and mobility of ions in cross-linked lysozyme crystals.
Micromethods for measurements of electric conductivity, transference numbers and concentrations of inorganic ions within immobilized protein crystals have been developed and applied to study tetragonal lysozyme crystals cross-linked with glutaraldehyde. Donnan equilibria and mobilities of ions in this crystal were calculated using the data of these methods and the data of crystal pH titration. Taken together these results characterize the lysozyme crystal as an ion exchanger whose electrical properties and ion composition differ greatly from those of the external solution. Although anions transfer most of the current in the crystals, anion mobility is considerably lower than that of cations. Mobility of all ions in the crystal is considerably lower than in solution (3.5-50 times for cations and 120-330 times for anions) and depends on steric restrictions and charges of both ions and lysozyme molecules. Similar features in behavior of crystalline and biological channels are discussed.
Morozova TYa
Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow region, Russia. morozov@is2.nyu.edu
Kachalova
G S
GS
Lanina
N F
NF
Evtodienko
V U
VU
Botin
A S
AS
Shlyapnikova
E A
EA
Morozov
V N
VN
D055598:Chemical Phenomena; D002627:Chemistry, Physical; D003432:Cross-Linking Reagents / Q000737:chemistry; D003460:Crystallization; D004553:Electric Conductivity; D004789:Enzyme Activation; D005976:Glutaral / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007473:Ion Channels / Q000737:chemistry; D007477:Ions; D009113:Muramidase / Q000737:chemistry*; D009994:Osmolar Concentration; D012965:Sodium Chloride / Q000737:chemistry
D003432:Cross-Linking Reagents; D007473:Ion Channels; D007477:Ions; D012965:Sodium Chloride; D009113:Muramidase; D005976:Glutaral