An ICAM-1 like cell adhesion molecule is responsible for CD34 positive haemopoietic stem cells adhesion to bone-marrow stroma.
The microenvironment in the haematopoietic organs plays an important role in regulating and sustaining differentiation and self-renewal of haematopoietic stem cells. Although crucial for stem cell maintenance and homing, the stromal cell-stem cell interactions are poorly understood. Here we show that an ICAM-like molecule is responsible for stem cell adhesion to stromal cells in vitro. The molecule was characterized by a monoclonal antibody 3E10. Immunoblotting results indicated that the molecule had an electrophoretic mobility equal to that of intercellular cell adhesion molecule-1 (ICAM-1). Binding inhibition assays, however, showed that inhibition of binding of enriched CD34 cells by 3E10 was more prominent in comparison with that of ICAM-1.
Rao
S G
SG
Chemotherapy & Stem Cell Biology Division, Cancer Research Institute, Parel, Bombay, India.
Chitnis
V S
VS
Deora
A
A
Tanavde
V
V
Desai
S S
SS
D000911:Antibodies, Monoclonal; D018952:Antigens, CD34 / Q000032:analysis*; D001854:Bone Marrow Cells; D002448:Cell Adhesion / Q000502:physiology; D002478:Cells, Cultured / Q000737:chemistry / Q000166:cytology; D003470:Culture Media; D005434:Flow Cytometry; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000276:immunology; D006801:Humans; D015151:Immunoblotting; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis / Q000276:immunology / Q000502:physiology*; D017154:Stromal Cells / Q000737:chemistry / Q000166:cytology
D000911:Antibodies, Monoclonal; D018952:Antigens, CD34; D003470:Culture Media; D018799:Intercellular Adhesion Molecule-1