New forms of HMW MAP2 are preferentially expressed in the spinal cord.
The high molecular weight forms of microtubule-associated protein 2 (MAP2a and b) play a central role in the specification of dendrites. RT-PCR amplification of a portion of the N-terminal and middle MAP2b domains of rat spinal cord cDNAs allowed identification of new variants containing both exon 8 (246 bp) and a new exon, 7A (237 bp), located at the beginning of the middle MAP2b region. The brain and the spinal cord express transcripts containing exon 8, whereas exon 7A alone or exons 7A+8 were detected, whatever the developmental stage, only in the spinal cord.
Couchie
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D
INSERM U 282-CNRS, 'Hormones and Cell Differentiation', Hôpital Henri Mandor, Créteil, France.
Chabas
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S
Mavilia
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C
Nunez
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J
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001923:Brain Chemistry; D003001:Cloning, Molecular; D005091:Exons / Q000235:genetics; D015870:Gene Expression; D018507:Gene Expression Regulation, Developmental; D014644:Genetic Variation / Q000235:genetics*; D008869:Microtubule-Associated Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis*; D051381:Rats; D017422:Sequence Analysis, DNA; D013116:Spinal Cord / Q000737:chemistry*
D008869:Microtubule-Associated Proteins; D012333:RNA, Messenger