In-vitro activity of cephalosporins alone and combined with sulbactam against various strains of Acinetobacter baumannii with different antibiotic resistance profiles.
The beta-lactamases of six strains of Acinetobacter baumannii were investigated using analytical isoelectric focusing, and the MIC values for 16 beta-lactam antibiotics determined against the strains. Three strains produced a chromosomal cephalosporinase (pI > 8.5), one strain a CARB-5 beta-lactamase (pI = 6.35), one strain a TEM-1 penicillinase (pI = 5.4) as well as a cephalosporinase (pI > 8.5), and one carbapenem-resistant strain a beta-lactamase with a pI > 8.5. Ceftazidime, cefepime, cefpirome, imipenem and meropenem were found to be the most effective beta-lactams against five strains. Sulbactam was active against four of the strains at 1-4 mg/L and showed enhanced killing effects with cefpirome.
Aubert
G
G
Department of Bacteriology, CHRU Bellevue Hospital, Saint-Etienne, France.
Guichard
D
D
Vedel
G
G
D000150:Acinetobacter / Q000187:drug effects*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002511:Cephalosporins / Q000494:pharmacology*; D004352:Drug Resistance, Microbial; D008826:Microbial Sensitivity Tests; D013407:Sulbactam / Q000494:pharmacology*; D001618:beta-Lactamases / Q000378:metabolism
D000900:Anti-Bacterial Agents; D002511:Cephalosporins; D001618:beta-Lactamases; D013407:Sulbactam