title: Differential expression of N-CAM, vimentin and MAP1B during initial pathfinding of olfactory receptor neurons in the mouse embryo.
abstract: Olfactory receptor neurons extend their primary axons from the nasal epithelium to the olfactory bulb primordium via the frontonasal mesenchyme. In the present study, expression of neuronal markers (vimentin and MAP1B) and N-CAM was immunohistochemically investigated in the development of the olfactory system in mouse embryos. Expression of vimentin and MAP1B was first observed at early day 10 of gestation (D10) in the posterosuperior region of the medial nasal epithelium, while N-CAM was initially detected in the mesenchyme adjacent to the vimentin- and MAP1B-positive nasal epithelium. As development proceeded, the localization of neuronal marker-positive cells was mostly included in the N-CAM positive region. In addition, we adopted in situ labelling with vital dye (DiI) to directly determine the localization of the olfactory nerve and N-CAM on the same sections. We demonstrated that most extending axons were located in the N-CAM positive region. These results suggest that the expression of N-CAM plays a crucial role in the initial pathfinding of the olfactory nerve.
authors.affiliation: Department of Developmental Biology, Graduate School of Dentistry, Tokyo Medical and Dental University, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Aoki
authors.forename: N
authors.initials: N
authors.lastname: Osumi-Yamashita
authors.forename: Y
authors.initials: Y
authors.lastname: Ninomiya
authors.forename: K
authors.initials: K
authors.lastname: Eto
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis; D015816:Cell Adhesion Molecules, Neuronal / Q000032:analysis*; D051379:Mice; D008810:Mice, Inbred C57BL; D008869:Microtubule-Associated Proteins / Q000032:analysis*; D009830:Olfactory Bulb / Q000737:chemistry / Q000196:embryology; D009831:Olfactory Mucosa / Q000737:chemistry / Q000196:embryology; D009833:Olfactory Pathways / Q000737:chemistry* / Q000196:embryology*; D018034:Olfactory Receptor Neurons / Q000737:chemistry / Q000196:embryology; D014746:Vimentin / Q000032:analysis*
chemical_list: D015415:Biomarkers; D015816:Cell Adhesion Molecules, Neuronal; D008869:Microtubule-Associated Proteins; D014746:Vimentin; C085284:microtubule-associated protein 1B