Expression of heparin-binding EGF-like growth factor in rat liver injured by carbon tetrachloride or D-galactosamine.
We reported recently that heparin-binding EGF-like growth factor (HB-EGF), a member of the EGF family, is a new hepatotrophic factor for the regeneration of rat liver after partial hepatectomy. The current study examined changes in the amount of HB-EGF mRNA in liver injured by hepatotoxins. The level of HB-EGF mRNA was very low in normal rat liver, but increased markedly in the liver of rats injured by CCl4, showing two peaks, the first at 6 h and the second at 36 h. Western blot analysis showed that HB-EGF protein in the liver of CCl4-treated rats (at 6 h) was increased about 3.4-fold above normal. The level of HB-EGF mRNA also increased markedly in the liver of rats treated with D-galactosamine, showing a major peak at 18 h, and a smaller one at 6 h. These results indicate that HB-EGF may play a role in the regeneration of the liver following hepatotoxic injury.
Kiso
S
S
Second Department of Internal Medicine, Osaka University Medical School, Japan.
Kawata
S
S
Tamura
S
S
Ito
N
N
Tsushima
H
H
Yamada
A
A
Higashiyama
S
S
Taniguchi
N
N
Matsuzawa
Y
Y
D000818:Animals; D015153:Blotting, Western; D002251:Carbon Tetrachloride; D056486:Chemical and Drug Induced Liver Injury; D004815:Epidermal Growth Factor / Q000235:genetics* / Q000378:metabolism; D005688:Galactosamine; D015870:Gene Expression; D006493:Heparin / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008107:Liver Diseases / Q000378:metabolism; D008297:Male; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
D012333:RNA, Messenger; D004815:Epidermal Growth Factor; D005688:Galactosamine; D006493:Heparin; D002251:Carbon Tetrachloride