Extraction of high quality DNA from bloodstains using diatoms.
A simple method is described for the extraction of high quality DNA for PCR amplification. The DNA was extracted by using Chelex-100 ion exchange resin or a special cell lysis buffer containing proteinase K. For further purification the DNA was bound to silica in the presence of a chaotrophic agent. Hence it is possible to unlimitedly wash the bound DNA and inhibitory substances are removed. By using diatoms as a source of silicates, this method is very economical and can therefore be used as a routine method.
Günther
S
S
Institut für Rechtsmedizin, Julius-Maximilians-Universität, Würzburg, Germany.
Herold
J
J
Patzelt
D
D
D001801:Blood Stains; D004247:DNA / Q000302:isolation & purification*; D017377:Diatoms; D019286:Endopeptidase K; D006801:Humans; D016133:Polymerase Chain Reaction / Q000191:economics / Q000379:methods*; D012697:Serine Endopeptidases
D004247:DNA; D012697:Serine Endopeptidases; D019286:Endopeptidase K