Cloning of the human homologue of the TGF beta-stimulated clone 22 gene.
We have isolated the human homologue if the TGF beta-stimulated clone 22 gene from a human embryo cDNA library. This gene maps to the q14 region of chromosome 13. Its deduced amino acid sequence is almost 99% identical to that of mouse or rat proteins and includes a putative leucine zipper motif. An abundant major transcript of 1.8 kb and in some instances an additional 5 kb transcript were detected by Northern blotting of several human tissues. The TSC-22 protein has been shown to be well conserved across evolution as evidenced by the existence of a Drosophila homologue. These observations prompt discussion on the strong conservation of TSC-22 during evolution but also on its general function as a primary response gene expressed either when stimulated by several different factors during early human development, or in the adult in response to inducing differentiation signals.
Jay
P
P
Centre de Recherches de Biochimie Macromoléculaire, CNRS UPR9008-INSERM U249, Montpellier, France.
Ji
J W
JW
Marsollier
C
C
Taviaux
S
S
Bergé-Lefranc
J L
JL
Berta
P
P
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D002882:Chromosomes, Human, Pair 13; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D015870:Gene Expression; D017781:Genes, Immediate-Early; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D011506:Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D012097:Repressor Proteins; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013552:Swine; D016212:Transforming Growth Factor beta / Q000494:pharmacology*
D018076:DNA, Complementary; D011506:Proteins; D012333:RNA, Messenger; D012097:Repressor Proteins; C099726:TSC22D1 protein, human; C490983:Tgfb1i4 protein, mouse; D016212:Transforming Growth Factor beta; C490982:Tsc22d1 protein, rat