Mapping of DNA-binding proteins along the yeast genome by UV-induced DNA-protein crosslinking.
UV-induced crosslinking of DNA-binding proteins to DNA in intact nuclei of Saccharomyces cerevisiae and subsequent 'protein image' hybridization were applied to map non-histone proteins along single-copy genes of yeast. We detected two polypeptides that most probably correspond to core subunits of yeast RNA-polymerase II in the coding region of transketolase gene (TKL2). Several non-histone proteins were also detected which bind to the upstream region of TKL2 gene, and to the intergenic spacer between calmodulin (CMD1) and beta-mannosyl transferase (ALG1) genes.
Papatsenko
D A
DA
W.A. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia.
Priporova
I V
IV
Belikov
S V
SV
Karpov
V L
VL
D002147:Calmodulin / Q000235:genetics; D004271:DNA, Fungal / Q000032:analysis / Q000378:metabolism* / Q000528:radiation effects; D004268:DNA-Binding Proteins / Q000032:analysis / Q000378:metabolism* / Q000528:radiation effects; D005800:Genes, Fungal; D016681:Genome, Fungal; D007438:Introns; D008364:Mannosyltransferases / Q000235:genetics; D012319:RNA Polymerase II / Q000032:analysis / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism*; D014466:Ultraviolet Rays
D002147:Calmodulin; D004271:DNA, Fungal; D004268:DNA-Binding Proteins; D008364:Mannosyltransferases; C035777:chitobiosyldiphosphodolichol beta-mannosyltransferase; D012319:RNA Polymerase II