Maintenance of pluripotency in mouse embryonic stem cells persistently infected with murine coronavirus.
A persistently coronavirus-infected embryonic stem (ES) cell line A3/MHV was established by infecting an ES cell line, A3-1, with mouse hepatitis virus type-2. Although almost all A3/MHV cells were found infected, both A3/MHV and A3-1 cells expressed comparable levels of cell surface differentiation markers. In addition, A3/MHV cells retained the ability to form embryoid bodies. These results suggest that persistent coronavirus infection does not affect the differentiation of ES cells.
Okumura
A
A
Department of Animal Pathology, Institute of Medical Science, University of Tokyo, Japan.
Machii
K
K
Azuma
S
S
Toyoda
Y
Y
Kyuwa
S
S
D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D004622:Embryo, Mammalian / Q000166:cytology; D005260:Female; D051379:Mice; D006517:Murine hepatitis virus / Q000502:physiology*; D011247:Pregnancy; D013234:Stem Cells / Q000166:cytology / Q000821:virology*