title: Ploidy as a prognostic feature in colonic adenocarcinoma.
abstract: OBJECTIVE
  To determine whether DNA content and cell-cycle kinetic characteristics in Dukes stage B colonic adenocarcinomas provide additional prognostic information in the context of clinicopathologic variables of known significance.
  
  
  METHODS
  Archival, paraffin-embedded tissue blocks from 210 Dukes B colonic adenocarcinomas were retrieved. After confirming stage, tumor cell nuclei were extracted, suspended, and stained. Cell nuclei from adjacent normal colon mucosa were used as controls.
  
  
  METHODS
  University-based, tertiary cancer referral center.
  
  
  METHODS
  Samples obtained from tumors resected at our institution between 1965 and 1984 were analyzed by flow cytometry for DNA index (DI) and percentages of cells in synthesis (S) phase (%S) and in G2 and mitosis (M) phases (%G2M). The data were correlated with 5-year survival. Follow-up was complete in all patients to at least 5 years.
  
  
  RESULTS
  Univariate analysis showed that the highest survival rates were associated with DI values near 1 and 2 (diploid and tetraploid tumors, P = .02) and the lowest %G2M values (tumors with fewer mitoses; P = .01). Five-year survival rates also differed significantly between patients with diploid (DI < 1.1) and those with aneuploid (1.1 < DI < 2) tumors (80% vs 64%, respectively; P = .02). Multivariate analysis revealed that race (P < .01), lymphatic or capillary microinvasion (P < .03), and ploidy (P < .05) were significantly associated with outcome. The influence of ploidy, race, and microinvasion on 5-year survival was estimated with logistic regression, and 8 subgroups of patients emerged with 5-year survival probabilities ranging from 39% for black patients with aneuploid tumors and microinvasion to 88% for white patients with diploid tumors and no microinvasion.
  
  
  CONCLUSIONS
  Tumor DNA content provides additional independent information that allows further refinement of our prognostic ability in patients with Dukes B colonic adenocarcinoma. This may aid in the identification of a cohort of patients who may potentially benefit from aggressive adjuvant therapy.
authors.affiliation: Department of Surgery, University of Chicago, Ill, USA.
authors.forename: D M
authors.initials: DM
authors.lastname: Takanishi
authors.forename: J
authors.initials: J
authors.lastname: Hart
authors.forename: P
authors.initials: P
authors.lastname: Covarelli
authors.forename: R
authors.initials: R
authors.lastname: Chappell
authors.forename: F
authors.initials: F
authors.lastname: Michelassi
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D000704:Analysis of Variance; D000782:Aneuploidy; D002453:Cell Cycle; D003106:Colon / Q000473:pathology; D003110:Colonic Neoplasms / Q000235:genetics* / Q000401:mortality* / Q000473:pathology; D004273:DNA, Neoplasm / Q000032:analysis; D004171:Diploidy; D005434:Flow Cytometry; D005500:Follow-Up Studies; D006801:Humans; D015999:Multivariate Analysis; D011003:Ploidies; D011123:Polyploidy; D011336:Probability; D011379:Prognosis; D013997:Time Factors
chemical_list: D004273:DNA, Neoplasm