title: A third P-domain peptide gene (TFF3), human intestinal trefoil factor, maps to 21q22.3.
abstract: Small peptides displaying a cysteine-rich module (termed P-domain or trefoil motif) form a recently increasing group of peptides abundantly expressed at mucosal surfaces of specific tissues and are associated with the maintenance of surface integrity. The estrogen-inducible pS2 gene (BCEI) and the human homolog to the porcine spasmolytic peptide (hsP) gene (SML1) appear synchronously expressed in healthy stomach mucosa and several carcinomas of the gastrointestinal tract. Both genes were shown to be located at 21q22.3. A new trefoil peptide from human intestinal mucosa (hITF/hP1.B) and its gene (TFF3) were described recently. By PCR analysis of a somatic cell hybrid panel and FISH using two large genomic recombinants (110 kb, 210 kb) cloned in the Bacterial Artificial Chromosome (BAC) system, we show that this gene coding for the new member of human P-domain/trefoil peptides also maps to chromosome region 21q22.3 suggesting a physical linkage of all three trefoil peptide genes.
authors.affiliation: Department of Anthropology and Human Genetics, University of Tübingen, Germany. holger.schmitt@uni-tuebingin.de
authors.forename: H
authors.initials: H
authors.lastname: Schmitt
authors.forename: I
authors.initials: I
authors.lastname: Wundrack
authors.forename: S
authors.initials: S
authors.lastname: Beck
authors.forename: P
authors.initials: P
authors.lastname: Gött
authors.forename: C
authors.initials: C
authors.lastname: Welter
authors.forename: H
authors.initials: H
authors.lastname: Shizuya
authors.forename: M I
authors.initials: MI
authors.lastname: Simon
authors.forename: N
authors.initials: N
authors.lastname: Blin
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001419:Bacteria / Q000235:genetics; D001483:Base Sequence; D002874:Chromosome Mapping; D002891:Chromosomes, Human, Pair 21 / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D008040:Genetic Linkage; D006133:Growth Substances / Q000235:genetics*; D006801:Humans; D006822:Hybrid Cells; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D009077:Mucins; D009124:Muscle Proteins; D009479:Neuropeptides; D010455:Peptides / Q000235:genetics*; D013552:Swine; D000071164:Trefoil Factor-2; D000071165:Trefoil Factor-3
chemical_list: D017931:DNA Primers; D006133:Growth Substances; D009077:Mucins; D009124:Muscle Proteins; D009479:Neuropeptides; D010455:Peptides; C000604007:TFF2 protein, human; C099037:TFF3 protein, rat; D000071164:Trefoil Factor-2; D000071165:Trefoil Factor-3