title: Purification and identification of allergenic alpha (2u)-globulin species of rat urine.
abstract: Amino-acid compositional and sequence analyses as well as mass spectrometric determinations of purified rat urine proteins, previously termed prealbumin and alpha(2)-euglobulin, have revealed a high homology between the two forms which have now been identified as alpha(2)-globulin species. The "prealbumin' fraction was found to correspond to alpha(2u)-globulin originating from salivary gland and the 'alpha(2)-euglobulin' fraction was identical with the major urinary protein (MUP) or alpha(2u)-globulin. The results indicate that the two major protein fractions of rat urine constitute different forms of the same parent protein, alpha(2u)-globulin, having no amino-acid sequence resemblance to prealbumin (transthyretin) of rat serum.
authors.affiliation: Division of Respiratory Allergy and Immunology, National Institute for Working Life, Solna, Sweden.
authors.forename: C
authors.initials: C
authors.lastname: Bayard
authors.forename: L
authors.initials: L
authors.lastname: Holmquist
authors.forename: O
authors.initials: O
authors.lastname: Vesterberg
mesh_terms: D000485:Allergens / Q000737:chemistry / Q000302:isolation & purification* / Q000652:urine; D000510:Alpha-Globulins / Q000737:chemistry / Q000276:immunology / Q000652:urine*; D000595:Amino Acid Sequence; D000818:Animals; D008297:Male; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011506:Proteins / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000485:Allergens; D000510:Alpha-Globulins; D011506:Proteins; C013803:alpha 2u globulin; C035737:major urinary proteins