[The use of proteases for enhancing the sensitivity of reactions to detect group isohemagglutinins in the forensic medical study of blood stains].
Group a and b isohemagglutinins are much better detected in blood stains by enzyme-treated, but not native red cells. This is specially true for antibodies of poorly dissolved strains, whose group antibodies can be detected virtually only by enzyme-treated red cells in papain extracts possessing the highest immunological activity. Two sensitive methods for the detection of isohemagglutinins have been developed: extract titration and modified Lattes' method, permitting a reliable detection of group antibodies in old blood stains, undetectable by routine methods.
Sakharov
R S
RS
Nofal'
Kh K
KhK
Druz'
A F
AF
Shchupik
Iu P
IuP
D000017:ABO Blood-Group System / Q000097:blood*; D001801:Blood Stains; D010450:Endopeptidases; D004912:Erythrocytes / Q000187:drug effects / Q000276:immunology; D005554:Forensic Medicine / Q000379:methods*; D006388:Hemagglutinins / Q000097:blood* / Q000187:drug effects; D006801:Humans; D007202:Indicators and Reagents; D012680:Sensitivity and Specificity; D013997:Time Factors
D000017:ABO Blood-Group System; D006388:Hemagglutinins; D007202:Indicators and Reagents; D010450:Endopeptidases
Primenenie proteaz dlia povysheniia chuvstvitel'nosti reaktsiĭ vyiavleniia gruppovykh izogemaggliutininov pri sudebno-meditsinskom issledovanii sledov krovi.