[Effect of microwave pretreatment on the detection of Epstein-Barr virus EBER RNA's using in situ hybridization].
We have tested the influence of microwave pretreatment of tissue sections on in situ hybridization applied to the detection of Epstein-Barr virus-encoded small RNAs (EBER). To this end, different protocols have been tested on slides of 4 lymph node lymphomas: microwave pretreatment, proteinase K pretreatment, microwave followed by proteinase K and no pretreatment. To compare the sensitivity of each technique, several dilutions of oligoprobes were used. Hybrids have been detected by immunohistochemistry. The analysis of the nuclear stainings obtained shows an enhancement of the sensitivity in protocols using microwaves.
Commo
F
F
Service d'Anatomie et de Cytologie Pathologiques, Hôpital Tenon, Paris.
Sibony
M
M
Antoine
M
M
Fouret
P
P
Callard
P
P
D019286:Endopeptidase K; D004854:Herpesvirus 4, Human / Q000302:isolation & purification* / Q000528:radiation effects; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008223:Lymphoma / Q000821:virology*; D008872:Microwaves; D012367:RNA, Viral / Q000302:isolation & purification* / Q000528:radiation effects; D012697:Serine Endopeptidases
D012367:RNA, Viral; D012697:Serine Endopeptidases; D019286:Endopeptidase K
Effets du prétraitement aux micro-ondes sur la détection des ARN EBER du virus d'Esptein-Barr (EBV) par hybridation in situ.