title: Requirement for CD8+ T cells in the development of airway hyperresponsiveness in a marine model of airway sensitization.
abstract: To study the role of CD8+ T cells in allergic sensitization, we examined the effects of in vivo depletion of CD8+ T cells prior to sensitization on IgE production, immediate type cutaneous hypersensitivity and development of altered airway responsiveness. BALB/c mice were thymectomized and treated with anti-CD8 antibody resulting in depletion of CD8+ T cells (<1%) in spleen and lymphoid tissues. In these mice, sensitization to ovalbumin (OVA) via the airways still resulted in IgE anti-OVA responses and immediate cutaneous reactions to OVA, but the animals were unable to develop airway hyperresponsiveness, eosinophil infiltration of the lung parenchyma, or IL-5 production in the local lymph nodes of the airway. Transfer of CD8+ T cells from naive animals during sensitization (on day 8 of the 10-d protocol) fully restored the ability to develop airway hyperresponsiveness and this was accompanied by IL-5 production and eosinophil accumulation in the lung. These data indicate a critical role for CD8+ T cells in the production of IL-5 and the development of altered airway responsiveness after antigen sensitization through the airways.
authors.affiliation: Division of Basic Sciences, Department of Pediatrics, University of Colorado Health Science Center, Denver 80206, USA.
authors.forename: E
authors.initials: E
authors.lastname: Hamelmann
authors.forename: A
authors.initials: A
authors.lastname: Oshiba
authors.forename: J
authors.initials: J
authors.lastname: Paluh
authors.forename: K
authors.initials: K
authors.lastname: Bradley
authors.forename: J
authors.initials: J
authors.lastname: Loader
authors.forename: T A
authors.initials: TA
authors.lastname: Potter
authors.forename: G L
authors.initials: GL
authors.lastname: Larsen
authors.forename: E W
authors.initials: EW
authors.lastname: Gelfand
mesh_terms: D000485:Allergens / Q000276:immunology; D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D004195:Disease Models, Animal; D004804:Eosinophils; D015848:Interleukin-5 / Q000096:biosynthesis; D007428:Intradermal Tests; D008168:Lung / Q000473:pathology; D008198:Lymph Nodes / Q000166:cytology / Q000378:metabolism; D008212:Lymphocyte Depletion; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000276:immunology; D012130:Respiratory Hypersensitivity / Q000209:etiology / Q000276:immunology*
chemical_list: D000485:Allergens; D015848:Interleukin-5; D010047:Ovalbumin