title: Requirement of multiple DNA-protein interactions for inducible expression of RNR3 gene in Saccharomyces cerevisiae in response to DNA damage.
abstract: The RNR3 gene encodes the large subunit of ribonucleotide reductase. Transcription of this gene is induced 12-fold in response to DNA damage or by a DNA replication blocker. To investigate cis-acting regulation, deletion analysis of the promoter region of the RNR3 gene was performed and we identified two upstream-repressing sequences in the RNR3 regulatory region. An 18-base-pairs fragment, termed DNA-damage responsive element 1 (DRE1) located between -212 and -194 in this region was found to be essential for the induction of RNR3. This fragment contained a negatively acting sequence where a protein factor bound to the region during normal growth but disappeared by exposure to 4-nitroquinoline-1-oxide. The other repressive element homologue to DRE1 was located at -263 to -254. One possible upstream-activating sequence which regulates the basal expression of RNR3 was also found. These results show that at least three potential cis-elements are necessary for the inducible expression of yeast expression of yeast RNR3 in response to DNA damage.
authors.affiliation: Department of Public Health, Kansai Medical University, Osaka, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Endo-Ichikawa
authors.forename: H
authors.initials: H
authors.lastname: Kohno
authors.forename: T
authors.initials: T
authors.lastname: Furukawa
authors.forename: T
authors.initials: T
authors.lastname: Ueda
authors.forename: Y
authors.initials: Y
authors.lastname: Ogawa
authors.forename: R
authors.initials: R
authors.lastname: Tokunaga
authors.forename: S
authors.initials: S
authors.lastname: Taketani
mesh_terms: D015112:4-Nitroquinoline-1-oxide / Q000494:pharmacology; D001482:Base Composition; D001483:Base Sequence; D004249:DNA Damage; D017931:DNA Primers; D015342:DNA Probes; D004261:DNA Replication; D004271:DNA, Fungal / Q000737:chemistry / Q000378:metabolism*; D004790:Enzyme Induction; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects; D005800:Genes, Fungal; D006918:Hydroxyurea / Q000494:pharmacology; D046911:Macromolecular Substances; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology; D008969:Molecular Sequence Data; D009153:Mutagens / Q000494:pharmacology; D016133:Polymerase Chain Reaction; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D012045:Regulatory Sequences, Nucleic Acid; D012264:Ribonucleotide Reductases / Q000096:biosynthesis*; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism*; D017384:Sequence Deletion; D014158:Transcription, Genetic; D001616:beta-Galactosidase / Q000096:biosynthesis
chemical_list: D017931:DNA Primers; D015342:DNA Probes; D004271:DNA, Fungal; D046911:Macromolecular Substances; D009153:Mutagens; D011993:Recombinant Fusion Proteins; D008769:Methylnitronitrosoguanidine; D015112:4-Nitroquinoline-1-oxide; D012264:Ribonucleotide Reductases; D001616:beta-Galactosidase; D006918:Hydroxyurea