Functional stability of porcine hepatocyte spheroids in various culture systems under 100% porcine and human plasma conditions.
To select an immobilization method suitable for bioartificial liver (BAL) modules utilizing porcine hepatocyte spheroids, functional activities were compared in various systems in 100% porcine and human plasma together with a synthesized medium. The spheroids, continuously suspended in rotating dishes or entrapped in collagen (CN) gel, expressed approximately two times higher ammonium detoxification abilities over conventional monolayers during 8 days of direct contact with 100% human or porcine plasma with a standardized inoculum cell number. No significant deterioration was observed in the abilities as compared with that in a synthesized medium. Although the cell number gradually decreased in rotational culture, the abilities per cells remaining on Day 10 were two times higher than in the CN-gel entrapped spheroids in all the media examined, presumably due to the diffusion limitation by the gel. Thus, in utilizing porcine hepatocyte spheroids in BAL modules, immobilization allowing direct contact of spheroids with perfused patient plasma was concluded to be possible and suitable.
Sakai
Y
Y
Fourth Department, Institute of Industrial Science, University of Tokyo, Japan.
Naruse
K
K
Nagashima
I
I
Muto
T
T
Suzuki
M
M
D000818:Animals; D001187:Artificial Organs; D001790:Blood Physiological Phenomena; D002452:Cell Count; D002478:Cells, Cultured; D003094:Collagen / Q000737:chemistry; D003470:Culture Media; D005456:Fluorescent Dyes / Q000737:chemistry; D005470:Fluorometry; D005782:Gels; D006801:Humans; D007211:Indoles / Q000737:chemistry; D008099:Liver / Q000166:cytology* / Q000502:physiology; D017114:Liver Failure, Acute / Q000628:therapy; D016031:Liver Transplantation; D000644:Quaternary Ammonium Compounds / Q000378:metabolism; D016060:Sorption Detoxification; D018874:Spheroids, Cellular / Q000166:cytology* / Q000502:physiology; D013552:Swine
D003470:Culture Media; D005456:Fluorescent Dyes; D005782:Gels; D007211:Indoles; D000644:Quaternary Ammonium Compounds; C007293:DAPI; D003094:Collagen