cDNA cloning, tissue distribution, and chromosomal localization of myelodysplasia/myeloid leukemia factor 2 (MLF2).
A fusion gene between nucleophosmin (NPM) and myelodysplasia/myeloid leukemia factor 1 (MLF1) is formed by a recurrent t(3;5)(q25.1;q34) in myelodysplastic syndrome and acute myeloid leukemia. Here we report the identification of a novel gene, MLF2, which contains an open reading frame of 744 bp encoding a 248-amino-acid protein highly related to the previously identified MLF1 protein (63% similarity, 40% identity). In contrast to the tissue-restricted expression pattern of MLF1, the MLF2 messenger RNA is expressed ubiquitously. The MLF2 gene locus was mapped by fluorescence in situ hybridization to human chromosome 12p13, a chromosomal region frequently involved in translocations and deletions in acute leukemias of lymphoid or myeloid lineage. In a physical map of chromosome 12, MLF2 was found to reside on the yeast artificial chromosome clone 765b9. Southern blotting analysis of malignant cell DNAs prepared from a series of acute lymphoblastic leukemia cases with translocations involving chromosome arm 12p, as well as a group of acute myeloid leukemias with various cytogenetic abnormalities, failed to reveal MLF2 gene rearrangements.
Kuefer
M U
MU
Department of Experimental Oncology,, St. Jude Children's Research Hospital, Memphis, Tennessee, 38101, USA.
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Williams
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Valentine
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Naeve
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Behm
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Mullersman
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Yoneda-Kato
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N
Montgomery
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Kucherlapati
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Morris
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D000595:Amino Acid Sequence; D001483:Base Sequence; D018797:Cell Cycle Proteins; D002869:Chromosome Aberrations; D025063:Chromosome Disorders; D002874:Chromosome Mapping; D002881:Chromosomes, Human, Pair 12; D002893:Chromosomes, Human, Pair 3; D002895:Chromosomes, Human, Pair 5; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D015870:Gene Expression; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007951:Leukemia, Myeloid / Q000235:genetics; D008969:Molecular Sequence Data; D005810:Multigene Family; D009369:Neoplasms / Q000235:genetics; D009687:Nuclear Proteins / Q000235:genetics; D000090243:Nucleophosmin; D016366:Open Reading Frames; D009928:Organ Specificity; D014176:Protein Biosynthesis; D011506:Proteins / Q000737:chemistry; D012333:RNA, Messenger / Q000096:biosynthesis; D017386:Sequence Homology, Amino Acid; D014178:Translocation, Genetic
D018797:Cell Cycle Proteins; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; C100852:MLF1 protein, human; C100853:MLF2 protein, human; C000716967:NPM1 protein, human; D009687:Nuclear Proteins; D011506:Proteins; D012333:RNA, Messenger; D000090243:Nucleophosmin