Spectrophotometric assay for processes involving changes in hydrogen ion concentration in aqueous solution.
A strategy for the design of simple colorimetric assays to follow any process that involves the net generation or consumption of hydrogen ions in aqueous solutions over the pH range of 3-10 is described. This procedure relies upon the measurement of the change in absorption when a weakly or moderately buffered solution of a pH indicator is subjected to a small change in pH. Buffers and indicators are chosen with closely matching pKa values. The versatility of this type of assay technique is illustrated using three examples.
Penketh
P G
PG
Department of Pharmacology and Developmental Therapeutics Program, Yale University School of Medicine, New Haven, Connecticut, 06520, USA.
Shyam
K
K
Patton
C L
CL
Sartorelli
A C
AC
D000477:Alkylating Agents / Q000737:chemistry; D000818:Animals; D002021:Buffers; D003124:Colorimetry / Q000379:methods; D005978:Glutathione / Q000737:chemistry; D006846:Hydrocarbons, Halogenated / Q000737:chemistry; D011522:Protons; D012996:Solutions; D013053:Spectrophotometry; D014346:Trypanosoma brucei brucei / Q000378:metabolism; D014867:Water / Q000737:chemistry*
D000477:Alkylating Agents; D002021:Buffers; D006846:Hydrocarbons, Halogenated; D011522:Protons; D012996:Solutions; D014867:Water; D005978:Glutathione