title: Direct database searching with MALDI-PSD spectra of peptides.
abstract: The analysis of matrix-assisted laser desorption ionization post-source decay (MALDI-PSD) mass spectra of peptides by using the cross-correlation method for database searching is illustrated. MALDI-PSD mass spectra are shown to contain sufficient fragmentation information to uniquely identify the correct amino acid sequence from large protein databases (approximately 160,000 entries). A search employing the MALDI-PSD mass spectrum of a phosphorylated peptide that correctly identifies the amino acid sequence and the site of phosphorylation is also illustrated.
authors.affiliation: Department of Inflammation Research, Merck Research Laboratories, Rahway, NJ 07065-0900, USA.
authors.forename: P R
authors.initials: PR
authors.lastname: Griffin
authors.forename: M J
authors.initials: MJ
authors.lastname: MacCoss
authors.forename: J K
authors.initials: JK
authors.lastname: Eng
authors.forename: R A
authors.initials: RA
authors.lastname: Blevins
authors.forename: J S
authors.initials: JS
authors.lastname: Aaronson
authors.forename: J R
authors.initials: JR
authors.lastname: Yates
mesh_terms: D000595:Amino Acid Sequence; D016208:Databases, Factual; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D008969:Molecular Sequence Data; D010455:Peptides / Q000032:analysis*; D010748:Phosphopeptides / Q000032:analysis
chemical_list: D010455:Peptides; D010748:Phosphopeptides