Determination of alternariol and alternariol methyl ether in apple juice using solid-phase extraction and high performance liquid chromatography.
The present work describes a new method for determination of alternariol (AOH) and alternariol methyl ether (AME) in apple juice using solid-phase extraction (SPE) columns for extraction and cleanup of samples for high-performance liquid chromatography (HPLC). Chromatograms of spiked samples show that both toxins can be easily detected without interferences, and good recoveries for AOH (82.8 +/- 7.4%) and AME (91.9 +/- 6.1%) with detection limits as low as 1.6 and 0.7 micrograms/l, respectively, were obtained.
Delgado
T
T
Instituto Fermentaciones Industriales, Consejo Superior de Investigaciones Científicas (C.S.I.C.), Madrid, Spain.
Gómez-Cordovés
C
C
Scott
P M
PM
D001628:Beverages / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005638:Fruit; D007783:Lactones / Q000032:analysis*; D009183:Mycotoxins / Q000032:analysis*; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
D007783:Lactones; D009183:Mycotoxins; C005197:alternariol; C018206:alternariol monomethyl ether