Forced over-expression of the myeloid zinc finger gene MZF-1 inhibits apoptosis and promotes oncogenesis in interleukin-3-dependent FDCP.1 cells.
The myeloid zinc finger protein MZF-1 is important in hematopoiesis. Previous studies have found that reducing expression of MZF-1 inhibited G-CSF-driven human marrow colony formation assays. In this study we found that retrovirally overexpressing MZF-1 in IL-3-dependent FDCP.1 cells inhibited their apoptosis when IL-3 was withdrawn. The MZF-1-transduced FDCP.1 cells also formed tumors when injected into congenic mice, whereas control FDCP.1 cells did not.
Hromas
R
R
Division of Hematology/Oncology, Indiana University Medical Center, Indianapolis 46202, USA.
Boswell
S
S
Shen
R N
RN
Burgess
G
G
Davidson
A
A
Cornetta
K
K
Sutton
J
J
Robertson
K
K
D000818:Animals; D017209:Apoptosis / Q000235:genetics*; D001853:Bone Marrow / Q000378:metabolism*; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D002478:Cells, Cultured; D004268:DNA-Binding Proteins / Q000235:genetics*; D015870:Gene Expression; D006801:Humans; D007377:Interleukin-3 / Q000494:pharmacology*; D051741:Kruppel-Like Transcription Factors; D051379:Mice; D009368:Neoplasm Transplantation; D014157:Transcription Factors / Q000235:genetics*; D016335:Zinc Fingers / Q000235:genetics*
D004268:DNA-Binding Proteins; D007377:Interleukin-3; D051741:Kruppel-Like Transcription Factors; C085969:MZF1 protein, human; C490095:Mzf1 protein, mouse; D014157:Transcription Factors