Selective induction of cytokines in mouse brain infected with canine distemper virus: structural, cellular and temporal expression.
We have previously shown that, in experimentally inoculated mice, canine distemper virus (CDV), a neurotropic virus, selectively infects certain brain structures (hypothalamus, hippocampus, monoaminergic nuclei, etc). Here we demonstrate that tumor necrosis factor (TNF)-alpha, interleukin (IL)-1 beta and IL-6 transcripts are selectively expressed in these CDV-targeted structures, except in the dentate gyrus, where cytokines are induced without prior CDV replication. The time-course of TNF-alpha expression vs. viral replication in the hypothalamus was different from that in hippocampus. In addition, we show that a substantial number of neurons express TNF-alpha and IL-6. These findings provide new insights into the possible participation of cytokines in the neurological disorders triggered by CDV infection.
Bencsik
A
A
INSERM U433, Neurobiologie Expérimentale et Physiopathologie, Faculté de Médecine A. Carrel, Lyon, France bencsik@cimac-res.univ-lyonl.fr
Malcus
C
C
Akaoka
H
H
Giraudon
P
P
Belin
M F
MF
Bernard
A
A
D000818:Animals; D001483:Base Sequence; D016207:Cytokines / Q000235:genetics* / Q000276:immunology; D004217:Distemper Virus, Canine / Q000235:genetics / Q000276:immunology*; D005260:Female; D005786:Gene Expression Regulation; D015967:Gene Expression Regulation, Viral / Q000276:immunology; D006624:Hippocampus / Q000166:cytology / Q000276:immunology* / Q000821:virology; D007031:Hypothalamus / Q000166:cytology / Q000276:immunology* / Q000821:virology; D017403:In Situ Hybridization; D007375:Interleukin-1 / Q000235:genetics / Q000276:immunology; D015850:Interleukin-6 / Q000235:genetics / Q000276:immunology; D051379:Mice; D008969:Molecular Sequence Data; D018185:Morbillivirus Infections / Q000276:immunology*; D009474:Neurons / Q000276:immunology / Q000821:virology; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000276:immunology
D016207:Cytokines; D007375:Interleukin-1; D015850:Interleukin-6; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha