Bone morphogenetic protein-12 and -13 inhibit terminal differentiation of myoblasts, but do not induce their differentiation into osteoblasts.
Effects of bone morphogenetic protein (BMP)-12 and BMP-13, new members of the BMP family which belong to the transforming growth factor (TGF)-beta superfamily, on terminal differentiation of myoblasts were examined in C2C12 and L-6 myoblasts. When the myoblasts were cultured with BMP-12 or BMP-13, the expression of the myosin heavy chain and the formation of multinucleated myotubes mRNA in L-6 cells. The inhibitory effects of BMP-12 and BMP-13 on myogenic differentiation were similar to the effects of BMP-2, though their potencies were lower than BMP-2. Unlike BMP-2, neither BMP-12 nor BMP-13 induced alkaline phosphatase activity in C2C12 myoblasts. The differences in the biological activities of these new BMPs suggest that the intracellular signalling pathway used by BMP-12 and BMP-13 differs from that of BMP-2.
Inada
M
M
Department of Biochemistry, School of Dentistry, Showa University, Tokyo, Japan.
Katagiri
T
T
Akiyama
S
S
Namika
M
M
Komaki
M
M
Yamaguchi
A
A
Kamoi
K
K
Rosen
V
V
Suda
T
T
D000469:Alkaline Phosphatase / Q000096:biosynthesis; D000818:Animals; D019485:Bone Morphogenetic Proteins; D002454:Cell Differentiation / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004790:Enzyme Induction; D006801:Humans; D007700:Kinetics; D051379:Mice; D018482:Muscle, Skeletal / Q000166:cytology* / Q000187:drug effects; D018995:Myosin Heavy Chains / Q000096:biosynthesis; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects; D011506:Proteins / Q000494:pharmacology*; D012333:RNA, Messenger / Q000096:biosynthesis; D011994:Recombinant Proteins / Q000494:pharmacology; D016212:Transforming Growth Factor beta / Q000494:pharmacology
D019485:Bone Morphogenetic Proteins; D011506:Proteins; D012333:RNA, Messenger; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; D000469:Alkaline Phosphatase; D018995:Myosin Heavy Chains