title: Ethanol-induced inhibition of [3H]thienylcyclohexylpiperidine (TCP) binding to NMDA receptors in brain synaptic membranes and to a purified protein complex.
abstract: N-Methyl-D-aspartate receptors (NMDARs) are a major target of ethanol effects in the nervous system. Haloperidol-insensitive, but dizocilpine (MK-801)-sensitive, binding of N-[1-(2-[3H]thienyl)cyclohexyl]piperidine ([3H]TCP) to synaptic membranes has the characteristics of ligand interaction with the ion channel of NMDARs. In the present studies, ethanol produced a concentration-dependent decrease in the maximal activation of [3H]TCP binding to synaptic membranes by NMDA and Gly, but a moderate change in the activation by L-Glu when L-Glu was present at concentrations < 100 microM. However, ethanol (100 mM) inhibited completely the activation of [3H]TCP binding produced by high concentrations of L-Glu (200-400 microM). It also inhibited strongly the activation of [3H]TCP binding by spermidine or spermidine plus Gly. In a purified complex of proteins that has L-Glu-, Gly-, and [3H]TCP-binding sites, ethanol (100 mM) decreased significantly the maximal activation of [3H]TCP binding produced by either L-Glu or Gly. Activation constants (Kact) for L-Glu and Gly acting on the purified complex were 12 and 28 microM, respectively. Ethanol had no significant effect on the Kact of L-Glu but caused an increase in Kact of Gly. These studies have identified at least one protein complex in neuronal membranes whose response to both L-Glu and Gly is inhibited by ethanol. These findings may explain some of the effects of acute and chronic ethanol treatment on the function and expression of the subunits of this complex in brain neurons.
authors.affiliation: Department of Pharmcology, University of Kansas, Lawrence, 66045, USA.
authors.forename: E K
authors.initials: EK
authors.lastname: Michaelis
authors.forename: X
authors.initials: X
authors.lastname: Chen
authors.forename: D B
authors.initials: DB
authors.lastname: Joseph
authors.forename: M
authors.initials: M
authors.lastname: Hurlbert
authors.forename: K N
authors.initials: KN
authors.lastname: Kumar
authors.forename: M L
authors.initials: ML
authors.lastname: Michaelis
mesh_terms: D000818:Animals; D001923:Brain Chemistry / Q000187:drug effects / Q000502:physiology; D016291:Dizocilpine Maleate / Q000494:pharmacology; D018492:Dopamine Antagonists / Q000494:pharmacology; D000431:Ethanol / Q000494:pharmacology*; D018690:Excitatory Amino Acid Agonists / Q000494:pharmacology; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology; D018698:Glutamic Acid / Q000494:pharmacology; D005998:Glycine / Q000494:pharmacology; D006220:Haloperidol / Q000494:pharmacology; D013287:Illicit Drugs / Q000378:metabolism; D007700:Kinetics; D008297:Male; D008565:Membrane Proteins / Q000187:drug effects / Q000378:metabolism; D016202:N-Methylaspartate / Q000494:pharmacology; D010622:Phencyclidine / Q000031:analogs & derivatives* / Q000037:antagonists & inhibitors / Q000378:metabolism; D011485:Protein Binding / Q000502:physiology; D011506:Proteins / Q000302:isolation & purification; D051381:Rats; D017207:Rats, Sprague-Dawley; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism*; D012680:Sensitivity and Specificity; D013095:Spermidine / Q000494:pharmacology; D013570:Synaptic Membranes / Q000737:chemistry / Q000187:drug effects; D014316:Tritium / Q000378:metabolism
chemical_list: D018492:Dopamine Antagonists; D018690:Excitatory Amino Acid Agonists; D018691:Excitatory Amino Acid Antagonists; D013287:Illicit Drugs; D008565:Membrane Proteins; D011506:Proteins; D016194:Receptors, N-Methyl-D-Aspartate; D014316:Tritium; D000431:Ethanol; D018698:Glutamic Acid; D016202:N-Methylaspartate; D016291:Dizocilpine Maleate; C012058:tenocyclidine; D010622:Phencyclidine; D006220:Haloperidol; D005998:Glycine; D013095:Spermidine