Bilirubin glucuronidation by intact Gunn rat fibroblasts expressing bilirubin UDP-glucuronosyltransferase.
Crigler-Najjar (CN) disease is an inherited disorder of bilirubin metabolism. The disease is caused by a deficiency of the hepatic enzyme bilirubin UDP-glucuronosyltransferase (B-UGT). Patients with CN disease have high serum levels of the toxic compound, unconjugated bilirubin. The only defect in bilirubin metabolism of CN patients is the absence of B-UGT activity. The transplantation of cells able to glucuronidate bilirubin should therefore lower serum bilirubin levels. The Gunn rat is the animal model of CN disease. Primary Gunn rat fibroblasts (GURF) were transduced with a recombinant retrovirus, capable of transferring B-UGT cDNA. A cell line was obtained expressing B-UGT at a level comparable to hepatocytes. Bilirubin added to the culture medium of these cells was glucuronidated and excreted. The B-UGT activities of transduced GURF and freshly isolated Wistar hepatocytes were compared at different bilirubin concentrations. The specific B-UGT activities of these two cell types were comparable when physiological bilirubin concentrations (5-10 microM) were present in the culture media. At higher bilirubin concentrations (20-80 microM) the hepatocytes were more active than the transduced GURF. We conclude that with the addition of only one enzyme (B-UGT) fibroblasts can perform the complete set of reactions necessary for bilirubin glucuronidation. The difference in B-UGT activity between transduced GURF and hepatocytes at 20-80 microM bilirubin can be explained by lower UDP-glucuronic acid and glutathione S-transferase levels in GURF. Our findings also indicate that these cells could be used to develop extrahepatic gene therapy for CN disease.
Seppen
J
J
Department of Gastrointestinal and Liver Disease, Academic Medical Centre, Amsterdam, The Netherlands.
Tada
K
K
Hellwig
S
S
Bakker
C T
CT
Prasad
V R
VR
Roy Chowdhury
N
N
Roy Chowdhury
J
J
Bosma
P J
PJ
Oude Elferink
R P
RP
D000818:Animals; D001663:Bilirubin / Q000378:metabolism*; D002999:Clone Cells; D005347:Fibroblasts / Q000201:enzymology / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D014453:Glucuronosyltransferase / Q000235:genetics* / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D008099:Liver / Q000166:cytology / Q000201:enzymology; D051381:Rats; D011911:Rats, Gunn; D017208:Rats, Wistar; D012190:Retroviridae / Q000235:genetics; D014162:Transfection
C019787:bilirubin glucuronoside glucuronosyltransferase; D014453:Glucuronosyltransferase; D005982:Glutathione Transferase; D001663:Bilirubin