title: In vitro import of pre-ferredoxin-NADP+-oxidoreductase from Cyanophora paradoxa into cyanelles and into pea chloroplasts.
abstract: Using a novel cyanelle isolation procedure we showed that pre-ferredoxin-NADP+-oxidoreductase (pre-FNR) from C. paradoxa is translocated in vitro across the peptidoglycan-containing cyanelle envelope. Efficient import was also observed in a heterologous system with pea chloroplasts as the recipient organelles. These results support the conclusion derived from comparative analysis of plastid genome organization, that all plastids originate from a common semi-autonomous endosymbiotic ancestor.
authors.affiliation: Institut für Biochemie und Molekulare Zellbiologie der Universität Wien und Ludwig-Boltzmann-Forschungsstelle für Biochemie, Austria.
authors.forename: J
authors.initials: J
authors.lastname: Jakowitsch
authors.forename: C
authors.initials: C
authors.lastname: Neumann-Spallart
authors.forename: Y
authors.initials: Y
authors.lastname: Ma
authors.forename: J
authors.initials: J
authors.lastname: Steiner
authors.forename: H E
authors.initials: HE
authors.lastname: Schenk
authors.forename: H J
authors.initials: HJ
authors.lastname: Bohnert
authors.forename: W
authors.initials: W
authors.lastname: Löffelhardt
mesh_terms: D002458:Cell Fractionation; D002498:Centrifugation; D002736:Chloroplasts / Q000378:metabolism*; D004792:Enzyme Precursors / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism*; D056890:Eukaryota / Q000201:enzymology* / Q000648:ultrastructure; D005287:Ferredoxin-NADP Reductase / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism*; D008970:Molecular Weight; D018532:Pisum sativum / Q000378:metabolism*; D010457:Peptidoglycan / Q000378:metabolism; D018087:Plastids / Q000201:enzymology* / Q000648:ultrastructure; D014158:Transcription, Genetic
chemical_list: D004792:Enzyme Precursors; D010457:Peptidoglycan; C098699:pre-ferredoxin-NADP-oxidoreductase; D005287:Ferredoxin-NADP Reductase