The utility of fluorescent in vivo reporter genes in molecular cardiology.
In vivo reporter genes can be used in different ways in molecular cardiology. In this paper studies are presented using the green fluorescent protein and one of its mutants, S65T-GFP, as in vivo reporter genes. With this new molecular tool we studied cell type specificity of the murine ventricular myosin light chain 2 promoter, positive cell identification prior to patch clamp procedures, and the use of fluorescence activated cell sorting of transiently transfected mammalian cells.
Doevendans
P A
PA
Department of Cardiology, Cardiovascular Research Institute Maastricht, The Netherlands.
Becker
K D
KD
An
R H
RH
Kass
R S
RS
D000818:Animals; D000831:Animals, Newborn; D015220:Calcium Channels / Q000096:biosynthesis / Q000502:physiology*; D020746:Calcium Channels, L-Type; D002460:Cell Line; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular / Q000379:methods; D005347:Fibroblasts; D049452:Green Fluorescent Proteins; D006352:Heart Ventricles; D006801:Humans; D007668:Kidney; D008164:Luminescent Proteins / Q000032:analysis / Q000096:biosynthesis*; D051379:Mice; D018994:Myosin Light Chains / Q000096:biosynthesis* / Q000235:genetics*; D018408:Patch-Clamp Techniques; D010957:Plasmids; D017354:Point Mutation; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000032:analysis / Q000096:biosynthesis*; D015183:Restriction Mapping; D012680:Sensitivity and Specificity; D014162:Transfection
D015220:Calcium Channels; D020746:Calcium Channels, L-Type; D008164:Luminescent Proteins; D018994:Myosin Light Chains; D011994:Recombinant Proteins; D049452:Green Fluorescent Proteins