The cDNA sequence and infectious transcripts of peanut stripe virus.
A full-length cDNA clone of the blotch isolate of the peanut stripe potyvirus (PStV) RNA genome was constructed downstream from the bacteriophage SP6 RNA polymerase promoter. The full-length PStV cDNA clone (PStVSF9) was sequenced and compared to the previously published sequence of PStV-B. In vitro-synthesized PStV transcripts capped with m7GpppG were infectious in Nicotiana benthamiana plants, and the progeny virus was aphid transmissible. To confirm the origin of infection, a mutant PStV (PStVDAE), with a Gly14 to Glu mutation in the coat protein-encoding gene, was constructed. Transcripts from PStVDAE produced symptoms indistinguishable from native or PStVSF9 virus, but was not transmitted by aphids.
Flasinski
S
S
Plant Biology Division, Samuel Roberts Noble Foundation, Ardmore, OK 73402, USA.
Gunasinghe
U B
UB
Gonzales
R A
RA
Cassidy
B G
BG
D010367:Arachis / Q000821:virology*; D001483:Base Sequence; D002213:Capsid / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D008969:Molecular Sequence Data; D009154:Mutation; D017800:Potyvirus / Q000235:genetics* / Q000472:pathogenicity; D014158:Transcription, Genetic
D018076:DNA, Complementary