Occurrence of nuclear-encoded tRNAIle in mitochondria of the liverwort Marchantia polymorpha.
Although 29 tRNA genes have been deduced from the complete nucleotide sequence of the mitochondrial genome from the liverwort Marchantia polymorpha, a tRNAIle gene decoding AUU and AUC codons is conspicuously absent. In order to address the question of the possible involvement of nuclear-encoded tRNA, we isolated and identified three variant copies of the nuclear-encoded tRNAIle(AAU) gene from the liverwort. Northern analysis showed the presence of nuclear-encoded tRNAIle both in the mitochondrion and the cytosol, while both chloroplast DNA-encoded tRNAIle and nuclear-encoded tRNATyr were absent in liverwort mitochondria. These results unequivocally establish that import of nuclear tRNAIle into mitochondria indeed occurs in one of the most primitive plants, M. polymorpha.
Akashi
K
K
Department of Agricultural Chemistry, Faculty of Agriculture, Kyoto University, Kyoto, 606-01, Japan.
Sakurai
K
K
Hirayama
J
J
Fukuzawa
H
H
Ohyama
K
K
D001483:Base Sequence; D001692:Biological Transport; D002467:Cell Nucleus / Q000235:genetics; D002478:Cells, Cultured; D003600:Cytosol / Q000378:metabolism; D018744:DNA, Plant; D008928:Mitochondria / Q000235:genetics*; D008969:Molecular Sequence Data; D010944:Plants; D018749:RNA, Plant; D012355:RNA, Transfer, Ile / Q000235:genetics* / Q000378:metabolism
D018744:DNA, Plant; D018749:RNA, Plant; D012355:RNA, Transfer, Ile