title: Casein kinase 2 inhibits the renaturation of complementary DNA strands mediated by p53 protein.
abstract: Considerable effort is currently being devoted to understand the functions of protein p53, a major regulator of cell proliferation. The protein p53 has been reported to catalyse the annealing of complementary DNA or RNA strands. We report that this activity is inhibited in the presence of the serine/threonine protein kinase CK2. It is shown that this inhibition can be explained by the occurrence of a high-affinity molecular association between p53 and CK2. The molecular complex involves an interaction between the C-terminal domain of p53 and the beta subunit of the oligomeric kinase. Accordingly, the isolated alpha subunit of the kinase was without effect. In addition, after phosphorylation by CK2, phosphorylated p53 lost its DNA annealing activity. Because the C-terminal domain of p53 is both involved in the association with CK2 and phosphorylated by it, our results suggest that either protein-protein interaction or phosphorylation of this domain might control the base pairing of complementary sequences promoted by p53 in processes related to DNA replication and repair.
authors.affiliation: CEA, Biochimie des Régulations Cellulaires Endocrines, INSERM Unit 244, Grenoble, France.
authors.forename: O
authors.initials: O
authors.lastname: Filhol
authors.forename: J
authors.initials: J
authors.lastname: Baudier
authors.forename: E M
authors.initials: EM
authors.lastname: Chambaz
authors.forename: C
authors.initials: C
authors.lastname: Cochet
mesh_terms: D000818:Animals; D047390:Casein Kinase II; D002460:Cell Line; D004247:DNA / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D004330:Drosophila; D006801:Humans; D007700:Kinetics; D046911:Macromolecular Substances; D009691:Nucleic Acid Denaturation; D009695:Nucleic Acid Renaturation; D017346:Protein Serine-Threonine Kinases / Q000302:isolation & purification / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D018411:Spodoptera; D014162:Transfection; D016159:Tumor Suppressor Protein p53 / Q000037:antagonists & inhibitors / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D046911:Macromolecular Substances; D011994:Recombinant Proteins; D016159:Tumor Suppressor Protein p53; D004247:DNA; D047390:Casein Kinase II; D017346:Protein Serine-Threonine Kinases