title: redD and actII-ORF4, pathway-specific regulatory genes for antibiotic production in Streptomyces coelicolor A3(2), are transcribed in vitro by an RNA polymerase holoenzyme containing sigma hrdD.
abstract: redD and actII-ORF4, regulatory genes required for synthesis of the antibiotics undecylprodigiosin and actinorhodin by Streptomyces coelicolor A3(2), were transcribed in vitro by an RNA polymerase holoenzyme containing sigma hrdD. Disruption of hrdD had no effect on antibiotic production, indicating that redD and actII-ORF4 are transcribed in vivo by at least one other RNA polymerase holoenzyme. These data provide the first experimental evidence that HrdD can function as a sigma factor.
authors.affiliation: Department of Genetics, Jonn Innes Centre, Norwich Research Park, Colney, United Kingdom.
authors.forename: T
authors.initials: T
authors.lastname: Fujii
authors.forename: H C
authors.initials: HC
authors.lastname: Gramajo
authors.forename: E
authors.initials: E
authors.lastname: Takano
authors.forename: M J
authors.initials: MJ
authors.lastname: Bibb
mesh_terms: D000595:Amino Acid Sequence; D000880:Anthraquinones / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D012321:DNA-Directed RNA Polymerases / Q000494:pharmacology*; D005798:Genes, Bacterial; D005809:Genes, Regulator; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D011353:Prodigiosin / Q000031:analogs & derivatives* / Q000096:biosynthesis; D012808:Sigma Factor / Q000502:physiology*; D013302:Streptomyces / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic; D000095622:Benzoisochromanequinones
chemical_list: D000880:Anthraquinones; D000900:Anti-Bacterial Agents; D012321:DNA-Directed RNA Polymerases; D011353:Prodigiosin; D012808:Sigma Factor; C047347:undecylprodigiosin; C013390:actinorhodin; D000095622:Benzoisochromanequinones