Hepatitis C virus sequences encoding truncated core proteins detected in a hepatocellular carcinoma.
RNA was specifically extracted from tumor and peritumor tissue of a hepatitis C virus (HCV)-associated hepatocellular carcinoma after microdissection. RT-PCR products of the HCV 5'-noncoding (NC), core and nonstructural (NS)-5 gene were examined. Nucleotide sequences of the core region derived from the tumor tissue revealed deletions and mutations resulting in truncated proteins. Peritumor and serum HCV sequences were unaffected.
Rüster
B
B
Chemotherapeutisches Forschungsinstitut Georg-Speyer-Haus, Frankfurt a. M., Germany.
Zeuzem
S
S
Roth
W K
WK
D000595:Amino Acid Sequence; D001483:Base Sequence; D006528:Carcinoma, Hepatocellular / Q000821:virology*; D017931:DNA Primers; D005814:Genes, Viral; D016174:Hepacivirus / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008113:Liver Neoplasms / Q000821:virology*; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009154:Mutation; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification*; D017384:Sequence Deletion; D014758:Viral Core Proteins / Q000096:biosynthesis* / Q000235:genetics; D017361:Viral Nonstructural Proteins / Q000096:biosynthesis / Q000235:genetics
D017931:DNA Primers; D012367:RNA, Viral; D014758:Viral Core Proteins; D017361:Viral Nonstructural Proteins