title: An alanine tRNA gene cluster from Nephila clavipes.
abstract: We report the sequence of a 2.3-kb genomic DNA fragment from the orb-web spider, Nephila clavipes (Nc). The fragment contains four regions of high homology to tRNA(Ala). The members of this irregularly spaced cluster of genes are oriented in the same direction and have the same anticodon (GCA), but their sequence differs at several positions. Initiation and termination signals, as well as consensus intragenic promoter sequences characteristic of tRNA genes, have been identified in all genes. tRNA(Ala) are involved in the regulation of the fibroin synthesis in the large ampullate Nc glands.
authors.affiliation: Department of Biology, University of Puerto Rico, San Juan 00931-3360.
authors.forename: E
authors.initials: E
authors.lastname: Luciano
authors.forename: G C
authors.initials: GC
authors.lastname: Candelas
mesh_terms: D000818:Animals; D001483:Base Sequence; D008969:Molecular Sequence Data; D005810:Multigene Family; D012344:RNA, Transfer, Ala; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D013112:Spiders / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D012344:RNA, Transfer, Ala