Identification and characterization of the pckA gene from Staphylococcus aureus.
The Staphylococcus aureus pckA gene was identified and characterized. A pckA mutant lacked detectable phosphoenolpyruvate carboxykinase activity and grew poorly in the absence of glucose. Both enzymatic activity and pckA promoter activity in wild-type cells grown in the absence of glucose were at least 22-fold greater than activities in cells grown in the presence of glucose.
Scovill
W H
WH
Department of Biological Sciences, University of Maryland Baltimore County, 21228, USA.
Schreier
H J
HJ
Bayles
K W
KW
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D010729:Phosphoenolpyruvate Carboxykinase (GTP) / Q000235:genetics*; D011401:Promoter Regions, Genetic; D013211:Staphylococcus aureus / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D014158:Transcription, Genetic
D010729:Phosphoenolpyruvate Carboxykinase (GTP)