The v-erbA oncogene selectively inhibits iodide uptake in rat thyroid cells.
v-erbA is the oncogenic form of the c-erbA proto-oncogene, which encodes the receptor for thyroid hormones. The expression of the v-erbA oncogene in thyroid differentiated cells, PC Cl 3, inhibits iodide uptake and thyrotropin-dependent growth, whereas it has no effect on the expression of the other thyroid specific markers, i.e. thyroglobulin, thyroperoxidase and thyrotropin receptor. The activity of transcription factor AP-1, evaluated by a specific DNA binding assay and by transcription of AP-induced promoter (TRE) is enhanced in PC v-erbA cells. v-erbA mutants in the DNA binding domain do not affect the iodide uptake of thyroid cells nor AP-1 activity. We suggest that this transcriptional activation mediates the selective effects of v-erbA on the expression of thyroid specific markers.
Trapasso
F
F
Dipartimento di Medicina Sperimentale e Clinica, Facoltà di Medicina eChirurgia di Cantanzaro, Università degli Studi di Reggio Calabria, Italy.
Martelli
M L
ML
Battaglia
C
C
Angotti
E
E
Mele
E
E
Stella
A
A
Samarut
J
J
Avvedimento
V E
VE
Fusco
A
A
D000818:Animals; D001483:Base Sequence; D002454:Cell Differentiation / Q000235:genetics; D002455:Cell Division / Q000235:genetics; D002461:Cell Line, Transformed; D002999:Clone Cells; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D018776:Genes, erbA; D007454:Iodides / Q000378:metabolism*; D008969:Molecular Sequence Data; D009154:Mutation; D010641:Phenotype; D051381:Rats; D011916:Rats, Inbred F344; D013961:Thyroid Gland / Q000166:cytology / Q000378:metabolism*; D018808:Transcription Factor AP-1 / Q000378:metabolism; D014162:Transfection
D004268:DNA-Binding Proteins; D007454:Iodides; D018808:Transcription Factor AP-1