Syntenic assignment of human serotonin receptor subtype 2 (HTR2), esterase D (ESD), and fms-related tyrosine kinase (FLT) homologs to bovine chromosome 12.
Bovine x rodent somatic hybrid cells have been used to syntenically map three bovine genes homologous to loci on human Chromosome (Chr) 13. These three loci, fms-related tyrosine kinase gene (FLT), esterase D (ESD), and 5-hydroxytryptamine receptor 2 (HTR2; serotonin receptor subtype 2), were assigned to bovine Chr 12 (BTA12) with 91-95% concordance to the coagulation factor 10 (F10) locus. Along with a previously mapped BTA12 gene, retinoblastoma-1 (RB1), this conserved synteny group spans 178 cM on human Chr 13 (HSA13). Previous reports suggested homology between HSA13 and both BTA2 and BTA12. Results reported here extend the boundary of the HSA13-BTA12 comparative map, contradict the previous preliminary assignment of ESD to BTA2, and suggest instead that the q arm of HSA13 may be entirely conserved in BTA12.
Sun
H S
HS
Department of Meat and Animal Science, University of Wisconsin-Madison, 1675 Observatory Drive, Madison, Wisconsin 53706, USA.
Womack
J E
JE
Kirkpatrick
B W
BW
D000818:Animals; D001483:Base Sequence; D043182:Carboxylesterase; D002265:Carboxylic Ester Hydrolases / Q000235:genetics*; D002417:Cattle / Q000235:genetics*; D002874:Chromosome Mapping; D002882:Chromosomes, Human, Pair 13; D017931:DNA Primers; D015894:Genome, Human; D006801:Humans; D006822:Hybrid Cells; D051379:Mice / Q000235:genetics; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D020794:Receptor Protein-Tyrosine Kinases / Q000235:genetics*; D017978:Receptors, Growth Factor / Q000235:genetics; D011985:Receptors, Serotonin / Q000235:genetics*; D012756:Sheep / Q000235:genetics; D013552:Swine / Q000235:genetics; D040281:Vascular Endothelial Growth Factor Receptor-1
D017931:DNA Primers; D011518:Proto-Oncogene Proteins; D017978:Receptors, Growth Factor; D011985:Receptors, Serotonin; D020794:Receptor Protein-Tyrosine Kinases; D040281:Vascular Endothelial Growth Factor Receptor-1; D002265:Carboxylic Ester Hydrolases; D043182:Carboxylesterase; C020118:ESD protein, human