Improved rapid method for the isolation, purification and identification of collagen glycosides.
An updated method for obtaining purified glucosylgalactosyl hydroxylysine (Glc-Gal-Hyl) is presented. Marine sponge is subjected to alkaline hydrolysis followed by ion-exchange chromatography and gel filtration. Identification is confirmed by consecutive mild acid hydrolysis of the extract to produce galactosyl hydroxylysine (Gal-Hyl) and hydroxylysine (Hyl). High-performance liquid chromatography (HPCL) with a sodium acetate-acetonitrile buffer and dabsyl chloride as the derivatizing agent demonstrated the purity of the extract and its hydrolyzates. Glc-Gal-Hyl is not commercially available but this easy and efficient method can provide large amounts of it for collagen metabolism studies.
Garza
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Collagen Research Laboratory, Houston, TX 77030, USA.
Bennett
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N
Rodriguez
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GP
D000818:Animals; D002240:Carbohydrate Sequence; D003094:Collagen / Q000737:chemistry*; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D011161:Porifera
D006027:Glycosides; D003094:Collagen