title: Dynamin II binds to the trans-Golgi network.
abstract: Two dynamin II-specific antibodies were used to detect dynamin II attached to highly purified Golgi membranes of HepG2 cells. Within the Golgi apparatus dynamin II is predominantly localized to the trans-Golgi network, as shown by immunoelectron microscopy. Increased binding of dynamin II to the trans-Golgi network in the presence of GTP-gamma-S and a reduced binding upon stimulation of secretion by phorbolester indicates that dynamin II may have a function in post-Golgi vesicle transport.
authors.affiliation: Division of Cell Biology, Max-Delbrück-Center for Molecular Medicine, Berlin, Germany.
authors.forename: O
authors.initials: O
authors.lastname: Maier
authors.forename: M
authors.initials: M
authors.lastname: Knoblich
authors.forename: P
authors.initials: P
authors.lastname: Westermann
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies; D015153:Blotting, Western; D002460:Cell Line; D034281:Dynamins; D004591:Electrophoresis, Polyacrylamide Gel; D020558:GTP Phosphohydrolases / Q000032:analysis / Q000378:metabolism*; D006056:Golgi Apparatus / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D016244:Guanosine 5'-O-(3-Thiotriphosphate) / Q000494:pharmacology; D006801:Humans; D007425:Intracellular Membranes / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D016253:Microscopy, Immunoelectron; D008870:Microtubules / Q000378:metabolism / Q000648:ultrastructure; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000737:chemistry / Q000276:immunology; D011817:Rabbits; D014407:Tumor Cells, Cultured
chemical_list: D000906:Antibodies; D010446:Peptide Fragments; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D020558:GTP Phosphohydrolases; D034281:Dynamins