45-kDa GTP-binding protein from rat olfactory epithelium: purification, characterization and localization.
The rat olfactory epithelium contains a specific water-soluble 45-kDa protein. This protein is recognized by anti-peptide antibodies which react with alpha-subunits of the known G-proteins. The 45-kDa protein has been isolated using DEAE-chromatography and gel-exclusion chromatography. The content of 45-kDa protein is about 2% of the total soluble proteins of the olfactory mucosa and it is located at the mucociliary surface. According to photo-affinity labeling, the 45-kDa protein possesses a high affinity to GTP and exhibits low GTP hydrolytic activity. The functions of the 45-kDa protein are discussed.
Novoselov
V I
VI
Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region, Russia.
Peshenko
I V
IV
Evdokimov
V A
VA
Nikolaev
J V
JV
Matveeva
E A
EA
Fesenko
E E
EE
D000345:Affinity Labels; D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies; D004848:Epithelium / Q000737:chemistry; D019204:GTP-Binding Proteins / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification*; D006160:Guanosine Triphosphate / Q000737:chemistry; D015151:Immunoblotting; D008297:Male; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009831:Olfactory Mucosa / Q000737:chemistry*; D010761:Phosphorus Radioisotopes; D011817:Rabbits; D051381:Rats; D017208:Rats, Wistar; D014420:Turbinates
D000345:Affinity Labels; D000906:Antibodies; D010761:Phosphorus Radioisotopes; D006160:Guanosine Triphosphate; D019204:GTP-Binding Proteins