Bacterial mutagenic evaluation of Luxabendazole, a new broad spectrum anthelmintic, with the Salmonella typhimurium His- and the Escherichia coli Tryp- reversion tests.
Luxabendazole is a new benzimidazole carbamate chemotherapeutic agent, which has proved to be effective against adult and immature stages of the major gastrointestinal nematodes, trematodes and cestodes. The mutagenic properties of Luxabendazole were investigated in the in vitro Ames Salmonella and E. coli tests. The product was tested at concentrations of 0.5, 5, 50, 500, 1250 and 2500 micrograms/plate in the TA1535, TA1538, TA98 and TA100 strains of Salmonella typhimurium, and 0.5, 5, 50 and 500 micrograms/plate in the WP2, WP2uvrA- and its pKM 101-containing derivative CM891 (WP2 uvrA- pKM101) strains of Escherichia coli, with and without S9 microsomal activation (post-mitochondrial liver fraction from Wistar rats pretreated with Aroclor(R)). Positive and negative controls were included in each experiment. From the present study it can be concluded that Luxabendazole, over a dose range of 0.5-2500 micrograms/plate, is unlikely to present a mutagenic hazard, as demonstrated by the Ames test.
Ortiz
A I
AI
Departamento de Fisiologá, Farmacología y Toxicologia, Facultad de Veterinaria, Universidad de Leon, Spain.
Pollastrini
M T
MT
Barea
M
M
Ordóñez
D
D
D000818:Animals; D000871:Anthelmintics / Q000493:pharmacokinetics / Q000633:toxicity*; D001562:Benzimidazoles / Q000493:pharmacokinetics / Q000633:toxicity*; D001711:Biotransformation; D002219:Carbamates / Q000493:pharmacokinetics / Q000633:toxicity*; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism; D009152:Mutagenicity Tests / Q000379:methods*; D009153:Mutagens / Q000493:pharmacokinetics / Q000633:toxicity*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics
D000871:Anthelmintics; D001562:Benzimidazoles; D002219:Carbamates; D009153:Mutagens; C058092:luxabendazole