Heat-labile uracil-DNA glycosylase: purification and characterization.
A uracil-DNA glycosylase (UNG) from a psychrophilic marine bacterium (BMTU 3346) has been purified to apparent homogeneity. The enzyme has a molecular weight of 23400 Da. It is stable in complex buffers (containing glycerol/BSA), whereas it is heat-labile in dilute buffers (free of stabilizers) with a half-life of 2 min at 40 degrees C. Due to the thermolability, uracil-DNA glycosylase is suitable for application in the carryover prevention technique showing less residual activity and/or a slower reactivation rate than the usually applied UNG from Escherichia coli.
Sobek
H
H
Boehringer Mannheim GmbH, Penzberg, Germany.
Schmidt
M
M
Frey
B
B
Kaluza
K
K
D001419:Bacteria / Q000201:enzymology; D001483:Base Sequence; D045647:DNA Glycosylases; D004795:Enzyme Stability; D006207:Half-Life; D006358:Hot Temperature; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009699:N-Glycosyl Hydrolases / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D051981:Uracil-DNA Glycosidase
D045647:DNA Glycosylases; D009699:N-Glycosyl Hydrolases; D051981:Uracil-DNA Glycosidase