Protein patterns, osmolytes, and aldose reductase of L-929 cells exposed to hyperosmotic media.
L-929 cells acclimated to media made hyperosmotic (600 mosmol/kgH2O) by addition of NaCl, sorbitol, or mannitol show, on SDS-polyacrylamide gels, a markedly enhanced protein band at 40 kDa, most likely corresponding to the enzyme aldose reductase. The effect was not observed in cells acclimated to a medium rendered hyperosmotic by addition of proline. The major organic osmolyte accumulated is sorbitol in cells acclimated to high-sorbitol or high-NaCl medium, proline in cells acclimated to high-proline medium. Cells acclimated to any of these hyperosmotic media display unaltered Na+ levels and similarly increased K+ levels and decreased Cl-levels. These results are interpreted in terms of the mechanisms involved in aldose reductase induction and in regulation of the enzyme activity in long-term acclimation to hyperosmotic media.
Libioulle
C
C
Laboratory of Animal Physiology, University of Liège, Belgium.
Llabres
G
G
Gilles
R
R
D000222:Adaptation, Physiological; D000449:Aldehyde Reductase / Q000378:metabolism*; D000818:Animals; D007739:L Cells; D051379:Mice; D008970:Molecular Weight; D011506:Proteins / Q000737:chemistry / Q000378:metabolism; D013012:Sorbitol / Q000378:metabolism; D013997:Time Factors; D014882:Water-Electrolyte Balance
D011506:Proteins; D013012:Sorbitol; D000449:Aldehyde Reductase