5-Nitrosothio-2-nitrobenzoate: a reagent for the nitrosation of thiol groups in proteins.
The S-nitroso derivative of 5-thio-2-nitrobenzoate was synthesized from 5,5'-dithiobis(2-nitrobenzoic acid) and partially characterized. Although relatively unstable, it is easy to prepare and reacts very rapidly with thiols and thiol groups of proteins to give corresponding S-nitrosothiols and 5-thio-2-nitrobenzoate dianion. The latter's easy spectrophotometric detection makes such reactions easy to follow and to quantitate.
Studebaker
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Department of Biochemistry, The Ohio State University, 484 West 12th Avenue, Columbus, Ohio, 43210, USA.
Zhang
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Means
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D000818:Animals; D002417:Cattle; D007202:Indicators and Reagents / Q000138:chemical synthesis; D009603:Nitroso Compounds / Q000138:chemical synthesis; D011506:Proteins / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry; D013053:Spectrophotometry; D013438:Sulfhydryl Compounds / Q000737:chemistry*
C101007:5-nitrosothio-2-nitrobenzoate; D007202:Indicators and Reagents; D009603:Nitroso Compounds; D011506:Proteins; D013438:Sulfhydryl Compounds; D012710:Serum Albumin, Bovine