Selective reduction in alpha-hydroxypalmitic acid-containing sphingomyelin and concurrent increase in hydroxylated ceramides in murine skin tumors induced by an initiation-promotion regimen.
The sphingomyelin cycle is activated to accumulate ceramides in the process of epidermal differentiation. We found that sphingomyelin in the epidermis of 4 different murine strains gave three bands on TLC, the lower band containing alpha-hydroxypalmitic acid (C16h:0(alpha)). However, in the papillomas induced in the skin of SENCAR and SSIN mice by initiation with 7,12-dimethylbenz[a]anthracene followed by promotion with 12-O-tetradecanoylphorbol acetate, the concentration of C16h:0(alpha)-containing sphingomyelin was selectively diminished with a concomitant increase in the concentrations of the ceramides containing alpha-hydroxy fatty acids. These findings indicate a possible involvement of the selective hydrolysis of alpha-hydroxy fatty acid-containing sphingomyelin in the process of tumorigenesis in mouse skin.
Kitano
Y
Y
Department of Plastic Surgery, The University of Tokyo, Japan.
Iwamori
Y
Y
Kiguchi
K
K
DiGiovanni
J
J
Takahashi
T
T
Kasama
K
K
Niwa
T
T
Harii
K
K
Iwamori
M
M
D000818:Animals; D002518:Ceramides / Q000737:chemistry / Q000378:metabolism; D002855:Chromatography, Thin Layer; D004817:Epidermis / Q000737:chemistry*; D005227:Fatty Acids / Q000032:analysis*; D006868:Hydrolysis; D051379:Mice; D008815:Mice, Inbred Strains; D009374:Neoplasms, Experimental / Q000139:chemically induced / Q000737:chemistry; D010169:Palmitic Acids / Q000032:analysis; D010212:Papilloma / Q000139:chemically induced / Q000737:chemistry*; D012878:Skin Neoplasms / Q000139:chemically induced / Q000737:chemistry*; D013109:Sphingomyelins / Q000737:chemistry* / Q000378:metabolism
D002518:Ceramides; D005227:Fatty Acids; D010169:Palmitic Acids; D013109:Sphingomyelins; C024707:2-hydroxyhexadecanoic acid