Tissue specific expression of testis angiotensin converting enzyme is not determined by the -32 nonconsensus TATA motif.
Testis ACE is an isozyme of angiotensin-converting enzyme (ACE) made by male germ cells. These cells recognize a small intragenic promoter which contains a positive regulatory element, TCTTAT, at position -32. A probe containing this element was gel shifted to an identical location by rat testis and rat liver nuclear extracts; formation of the complex was blocked by anti-TATA binding protein antibody. Recombinant TATA binding protein recognized the testis ACE TCTTAT motif as well as a probe containing the consensus TATA motif. Mice transgenic for testis ACE promoter constructs containing either the wild type testis ACE motif or a consensus TATA sequence expressed a reporter gene at high levels only within the testis. These data suggest that the testis ACE motif TCTTAT is a non-consensus TATA, but is not responsible for the highly restricted pattern of testis ACE gene expression.
Zhou
Y
Y
Department of Pathology, Emory University, Atlanta, Georgia 30322, USA.
Overbeek
P A
PA
Bernstein
K E
KE
D000818:Animals; D000906:Antibodies; D001483:Base Sequence; D002467:Cell Nucleus / Q000378:metabolism; D016384:Consensus Sequence; D004268:DNA-Binding Proteins / Q000276:immunology / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D007438:Introns; D008099:Liver / Q000201:enzymology*; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D009928:Organ Specificity; D007703:Peptidyl-Dipeptidase A / Q000096:biosynthesis* / Q000235:genetics; D011401:Promoter Regions, Genetic; D051381:Rats; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000378:metabolism; D012045:Regulatory Sequences, Nucleic Acid; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D016385:TATA Box; D035181:TATA-Box Binding Protein; D013737:Testis / Q000201:enzymology*; D014157:Transcription Factors / Q000276:immunology / Q000378:metabolism
D000906:Antibodies; D004268:DNA-Binding Proteins; D011994:Recombinant Proteins; D035181:TATA-Box Binding Protein; D014157:Transcription Factors; D007703:Peptidyl-Dipeptidase A