A soluble preparation from developing groundnut seeds (Arachis hypogaea) catalyzes de novo synthesis of long chain fatty acids.
A 100,000 x g supernatant fraction prepared from developing groundnut seeds (30-35 days after flowering) catalyzed the synthesis of fatty acids from [l-14C]acetate at a rate of 120nmoles of acetate incorporated per hr per gram fresh weight of tissue. 90% of this incorporated label was associated with fatty acids. The major fatty acids formed were stearic- (77%) and palmitic acids (14%) with 4% of oleic acid. The fatty acid synthetase activity was stable when stored at 0-4 degrees C for at least fifteen days. It is concluded from these results that acetyl-coA carboxylase and all the enzymes of fatty acid synthetase from developing groundnut seeds are soluble.
Sreenivas
A
A
Department of Biochemistry, Indian Institute of Science, Bangalore, India.
Sastry
P S
PS
D010367:Arachis / Q000201:enzymology* / Q000254:growth & development; D002384:Catalysis; D005227:Fatty Acids / Q000138:chemical synthesis* / Q000737:chemistry; D012639:Seeds / Q000201:enzymology* / Q000254:growth & development; D012995:Solubility
D005227:Fatty Acids