title: The use of jejunal transplants to treat a genetic enzyme deficiency.
abstract: BACKGROUND
  The Gunn rat is an excellent animal model of Crigler-Najjar syndrome, type 1. The liver and small intestine synthesize no functional bilirubin uridine diphosphoglucuronosyl transferase and, consequently, the animals cannot conjugate bilirubin. In prior studies, the authors have shown that 15- to 20-cm jejunal transplants from normal Wistar rats lowered but did not normalize serum bilirubin levels. Phenobarbital has been used to increase enzyme conjugation of bilirubin.
  
  
  OBJECTIVE
  Phenobarbital treatment of Gunn recipients of jejunal transplants from Wistar rats normalizes serum bilirubin levels.
  
  
  METHODS
  Forty-three Gunn recipients of jejunal transplants from Wistar rats were divided into four groups: 1) heterotopically placed grafts (Thiry-Vella loops), saline-treated, n = 14; 2) heterotopically placed grafts, phenobarbital-treated (80 mg/kg/day), n = 17; 3) orthotopically placed (in intestinal continuity) grafts, saline-treated, n = 5; and 4) orthotopically placed grafts, phenobarbital-treated, n = 7. Serum was collected before operation and weekly for 8 weeks for measurement of serum total, indirect, and direct bilirubin levels. Animals received cyclosporine, 5 micrograms/kg, daily intramuscularly.
  
  
  RESULTS
  Phenobarbital significantly augmented the bilirubin-lowering effect of heterotopic jejunal transplants (group 2). Mean total serum bilirubin fell from 9.14 +/- 0.01 to a nadir of 1.63 +/- 0.11 mg/dL at 6 weeks, after which time, levels began to rise toward baseline (as noted previously). Serum indirect bilirubin levels behaved in a similar fashion. Phenobarbital treatment "normalized" serum bilirubin levels in recipients of orthotopic Wistar jejunal grafts (group 4). Mean total serum bilirubin plummeted from 8.41 +/- 0.20 to 0.76 +/- 0.15 mg/dL at 1 week, and levels remained within the normal range for the entire 8-week study period. Identical changes were observed for serum indirect bilirubin levels.
  
  
  CONCLUSIONS
  The combination of phenobarbital treatment and orthotopic small bowel transplantation may be an appropriate therapeutic alternative to liver transplantation in the management of Crigler-Najjar syndrome, type 1.
authors.affiliation: Department of Surgery, Tulane University School of Medicine, New Orleans, Louisiana, USA.
authors.forename: B M
authors.initials: BM
authors.lastname: Jaffe
authors.forename: A A
authors.initials: AA
authors.lastname: Burgos
authors.forename: M
authors.initials: M
authors.lastname: Martinez-Noack
mesh_terms: D000818:Animals; D001663:Bilirubin / Q000097:blood; D003414:Crigler-Najjar Syndrome / Q000097:blood / Q000601:surgery*; D004195:Disease Models, Animal; D007583:Jejunum / Q000637:transplantation*; D010634:Phenobarbital / Q000627:therapeutic use; D051381:Rats; D011911:Rats, Gunn; D017208:Rats, Wistar
chemical_list: D001663:Bilirubin; D010634:Phenobarbital