Identification of a human RAD52 pseudogene located on chromosome 2.
A human testis cDNA library was screened with a hybridization probe encoding the mouse RAD52 gene. Two classes of clones were identified, one derived from the human RAD52 homolog (hRAD52), the other derived from a pseudogene. In addition to many point mutations, several of which encode stop codons, the pseudogene contains a number of frame shifts and a 103-bp deletion. We further determined that the pseudogene is processed and is located on human chromosome 2, in contrast to hRAD52 which is found on chromosome 12. Reverse transcription-PCR analysis of cultured human diploid fibroblasts, as well as fibrosarcoma cells, revealed that while hRAD52 is expressed at low, but detectable levels in these cells, the pseudogene is not.
Johnson
B L
BL
Department of Pharmacology, University of Minnesota Medical School, Minneapolis 55455, USA.
Campbell
C
C
D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D002889:Chromosomes, Human, Pair 2 / Q000235:genetics*; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D015870:Gene Expression; D015723:Gene Library; D015894:Genome, Human; D006801:Humans; D006822:Hybrid Cells; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011544:Pseudogenes / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D013737:Testis / Q000502:physiology
D004268:DNA-Binding Proteins; D004247:DNA