title: The sequence of a small subunit of cytochrome c oxidase from Crithidia fasciculata which is homologous to mammalian subunit IV.
abstract: The sequence of subunit 8 of cytochrome c oxidase from Crithidia fasciculata was determined by sequencing cDNA and N-terminus of the mature protein (Mr = 15.7 kDA). The (inferred) protein is homologous to mammalian cox IV and the corresponding cox subunits from yeast, Neurospora crassa and Dictyostelium discoideum, which is reflected in a very similar hydropathy profile. Elements that are conserved in the C. fasciculata sequence include (i) an N-terminal (D/E)-(K/R)-X-K-(X2)-W-(X2)-(I/L) motif, (ii) a putative membrane-spanning region in the middle portion of the protein, and (iii) a C-terminal W-(X13)-(N/D)-P motif. The C. fasciculata protein is synthesized with a cleavable presequence.
authors.affiliation: E.C. Slater Institute, University of Amsterdam, Academic Medical Centre, The Netherlands.
authors.forename: D
authors.initials: D
authors.lastname: Speijer
authors.forename: C K
authors.initials: CK
authors.lastname: Breek
authors.forename: A O
authors.initials: AO
authors.lastname: Muijsers
authors.forename: P X
authors.initials: PX
authors.lastname: Groenevelt
authors.forename: H
authors.initials: H
authors.lastname: Dekker
authors.forename: A
authors.initials: A
authors.lastname: de Haan
authors.forename: R
authors.initials: R
authors.lastname: Benne
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017124:Conserved Sequence; D016831:Crithidia fasciculata / Q000201:enzymology*; D017931:DNA Primers; D018076:DNA, Complementary; D003576:Electron Transport Complex IV / Q000096:biosynthesis* / Q000737:chemistry* / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D046911:Macromolecular Substances; D008322:Mammals; D051379:Mice; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016133:Polymerase Chain Reaction; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification; D017386:Sequence Homology, Amino Acid
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D046911:Macromolecular Substances; D011994:Recombinant Proteins; D003576:Electron Transport Complex IV