title: Association between plasma membrane (Ca+Mg) ATPase and calpain/calpastatin system in rat erythrocytes.
abstract: We studied the activity of plasma membrane (Ca+Mg)ATPase from erythrocytes of Milan hypertensive rat strain (MHS) and Milan low calpastatin rat strain (MLCS), that show an activity level of the specific calpain inhibitor, calpastatin, about five fold reduced in comparison with the Milan normotensive rat strain (MNS), while the protease activity level is similar. This imbalance of calpain:calpastatin ratio leads to a decrease of the erythrocyte plasma membrane (Ca+Mg)ATPase activity and to the appearance of 124 kDa fragments, which are the typical products of proteolytic calpain action on the 136 kDa (Ca+Mg)ATPase native form.
authors.affiliation: Department of Sciences and Biochemical Technologies, University of Milano, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Soldati
authors.forename: G
authors.initials: G
authors.lastname: Vezzoli
authors.forename: S
authors.initials: S
authors.lastname: Salardi
authors.forename: R
authors.initials: R
authors.lastname: Spaventa
authors.forename: B R
authors.initials: BR
authors.lastname: Barber
authors.forename: T
authors.initials: T
authors.lastname: Azzani
authors.forename: G
authors.initials: G
authors.lastname: Bianchi
mesh_terms: D000818:Animals; D001794:Blood Pressure; D015153:Blotting, Western; D017301:Ca(2+) Mg(2+)-ATPase / Q000097:blood* / Q000302:isolation & purification; D002135:Calcium-Binding Proteins / Q000097:blood* / Q000302:isolation & purification; D002154:Calpain / Q000037:antagonists & inhibitors / Q000097:blood* / Q000302:isolation & purification; D004791:Enzyme Inhibitors / Q000097:blood; D004910:Erythrocyte Membrane / Q000201:enzymology / Q000378:metabolism*; D006973:Hypertension / Q000097:blood* / Q000235:genetics / Q000503:physiopathology; D008297:Male; D008970:Molecular Weight; D051381:Rats; D011922:Rats, Mutant Strains
chemical_list: D002135:Calcium-Binding Proteins; D004791:Enzyme Inhibitors; C033668:calpastatin; D002154:Calpain; D017301:Ca(2+) Mg(2+)-ATPase