Rapid detection of mitochondrial sequence polymorphisms using multiplex solid-phase fluorescent minisequencing.
This work describes a novel method, multiplex solid-phase fluorescent minisequencing, for the simultaneous detection of several point mutations and/or small deletions and insertions. The method is applied to the analysis of mitochondrial DNA polymorphisms for the purposes of individual identification. A database of 152 British Caucasians and 103 British Afro-Caribbeans has been constructed, and the probability of a chance match between two unrelated individuals is calculated as 0.054 for Caucasians and 0.026 for Afro-Caribbeans.
Tully
G
G
Service Development, The Forensic Science Service, Priory House, Birmingham, B5 6QQ, United Kingdom.
Sullivan
K M
KM
Nixon
P
P
Stones
R E
RE
Gill
P
P
D001483:Base Sequence; D044383:Black People / Q000235:genetics; D017931:DNA Primers; D004272:DNA, Mitochondrial / Q000737:chemistry / Q000235:genetics*; D005453:Fluorescence; D005828:Genetics, Population; D006239:Haplotypes; D006801:Humans; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D011336:Probability; D017422:Sequence Analysis, DNA / Q000379:methods*; D006113:United Kingdom / Q000208:ethnology; D044465:White People / Q000235:genetics
D017931:DNA Primers; D004272:DNA, Mitochondrial