L ferritin accumulation in macrophages infiltrating the lung during rat Angiostrongylus cantonensis infection.
A 22-kDa protein was increased quantitatively, as measured by sodium dodecyl sulfate-polyacrylamide gel electrophoresis techniques, in lung microsomes prepared from Angiostrongylus cantonensis-infected rats. However, it was almost absent in normal rats. The protein was purified by sequential chromatography on Superdex 200 columns and identified chemically and immunologically as ferritin. Using isoelectric focusing and anion exchange chromatography, it was identified as L ferritin. Distribution of this 22-kDa protein in the lung tissue of A. cantonensis-infected rate was studied by immunocytochemistry. Positively stained cells were mainly infiltrated macrophages. Our results suggest that L ferritin accumulation in the macrophages may be related to the proliferation of connective tissue elements and the inflammatory response to A. cantonensis dwelling in the pulmonary arteries of the rat.
Lee
H H
HH
Department of Parasitology, Chung-Shan Medical and Dental College, Taiwan, R.O.C.
Jiang
S T
ST
Shyu
L Y
LY
Lin
W L
WL
Chian
H C
HC
Hsu
C C
CC
Chou
F P
FP
Wang
C J
CJ
D017185:Angiostrongylus cantonensis; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D005293:Ferritins / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D007106:Immune Sera / Q000276:immunology; D015151:Immunoblotting; D007150:Immunohistochemistry; D007525:Isoelectric Focusing; D008168:Lung / Q000378:metabolism / Q000473:pathology*; D008264:Macrophages / Q000378:metabolism*; D008297:Male; D011817:Rabbits; D051381:Rats; D017208:Rats, Wistar; D017206:Strongylida Infections / Q000378:metabolism* / Q000473:pathology
D007106:Immune Sera; D005293:Ferritins