Molecular cloning and site-specific mutagenesis of a gene involved in arylsulfatase production in Campylobacter jejuni.
The arylsulfatase gene from Campylobacter jejuni 81-176 encodes a predicted protein of 69,293 Da which shows no sequence similarity with other known arylsulfatases. The gene hybridizes to other Ast+ strains of C. jejuni and Campylobacter sputorum subsp. bubulus, as well as to many Ast- strains of C. jejuni.
Yao
R
R
Enterics Program, Naval Medical Research Institute, Bethesda, Maryland 20814, USA.
Guerry
P
P
D000595:Amino Acid Sequence; D001192:Arylsulfatases / Q000096:biosynthesis / Q000235:genetics*; D001483:Base Sequence; D016123:Campylobacter jejuni / Q000201:enzymology / Q000235:genetics*; D003001:Cloning, Molecular; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D016366:Open Reading Frames; D010957:Plasmids
D001192:Arylsulfatases