title: Expression of glycine receptor subunits in the cochlear nucleus and superior olivary complex using non-radioactive in-situ hybridization.
abstract: The distribution of glycine receptor (GlyR) subunit mRNAs was examined in the cochlear nucleus (CN) and superior olivary complex of 5-6-week-old and 8-10-week-old rats using a non-radioactive in-situ hybridization method. In the younger rats, GlyR alpha 1-, alpha 2-, and alpha 3- and beta-subunits were observed in all major ventral cochlear nucleus (VCN) and superior olivary complex (SOC) neurons, while only alpha 1-, alpha 3- and beta-subunits were observed in dorsal cochlear nucleus (DCN) neurons. In 8-10-week-old rats, GlyR alpha 1-, alpha 3- and beta-subunits were observed in all major CN and SOC neurons, while mRNA for GlyR alpha 2-subunit was not observed. These results indicate that GlyR is being expressed all major CN and SOC neurons, with alpha 1-, alpha 3- and beta-subunit components of the mature receptor and the alpha 2-subunit, a component of the immature GlyR, which is not down-regulated until after 6 weeks of age in most CN and SOC neurons.
authors.affiliation: Kresge Hearing Research Institute, University of Michigan, Ann Arbor 48109, USA.
authors.forename: K
authors.initials: K
authors.lastname: Sato
authors.forename: H
authors.initials: H
authors.lastname: Kuriyama
authors.forename: R A
authors.initials: RA
authors.lastname: Altschuler
mesh_terms: D000375:Aging / Q000378:metabolism; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D017626:Cochlear Nucleus / Q000166:cytology / Q000378:metabolism*; D015870:Gene Expression; D017403:In Situ Hybridization; D008297:Male; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D015345:Oligonucleotide Probes; D009847:Olivary Nucleus / Q000166:cytology / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D018009:Receptors, Glycine / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D017557:Ventral Tegmental Area / Q000166:cytology / Q000378:metabolism*
chemical_list: D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D018009:Receptors, Glycine