Recombinant bovine somatotropin decreases hepatic amino acid catabolism in female rats.
The effect of recombinant bovine somatotropin (rbST) on hepatic amino acid catabolism in female rats was investigated. Daily injections of rbST for 5 d decreased liver homogenate lysine alpha-ketoglutarate reductase (EC 1.5.1.8) activity (P < 0.05) and liver homogenate lysine oxidation (P < 0.05) approximately 35%. Liver homogenate methionine and valine oxidation were depressed approximately 20 (P = 0.13) and 35% (P < 0.05), respectively. These data show a decrease in hepatic capacity to oxidize amino acids in rats administered rbST. Whether depressed liver amino acid degrading enzyme activity plays a role in amino acid oxidation in vivo remains to be evaluated.
Blemings
K P
KP
Department of Meat and Animal Science, University of Wisconsin-Madison, Madison, WI 53706, USA.
Gahl
M J
MJ
Crenshaw
T D
TD
Benevenga
N J
NJ
D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D005260:Female; D013006:Growth Hormone / Q000494:pharmacology*; D008099:Liver / Q000033:anatomy & histology / Q000187:drug effects* / Q000378:metabolism*; D008239:Lysine / Q000378:metabolism; D008715:Methionine / Q000378:metabolism; D009929:Organ Size / Q000187:drug effects; D010084:Oxidation-Reduction; D051381:Rats; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins / Q000494:pharmacology; D012443:Saccharopine Dehydrogenases / Q000378:metabolism; D014633:Valine / Q000378:metabolism; D015430:Weight Gain / Q000187:drug effects
D000596:Amino Acids; D011994:Recombinant Proteins; D013006:Growth Hormone; D008715:Methionine; D012443:Saccharopine Dehydrogenases; C029496:saccharopine dehydrogenase (NADP+, lysine-forming); D014633:Valine; D008239:Lysine