title: The differential role of CD86 and CD80 co-stimulatory molecules in the induction and the effector phases of contact hypersensitivity.
abstract: Contact hypersensitivity (CH) has served as a useful model for investigating the allergen-specific immune responses of T cells and skin-associated antigen-presenting cells. We examined the distinct role between CD80 and CD86 on hapten-induced CH in both an induction and an effector phase. Intraperitoneal injection of mAb against CD86, but not CD80, 2 h before sensitization with epicutaneous application of dinitrofluorobenzene led to an almost complete inhibition of ear swelling, and histologically a marked reduction of edema, inflammatory polymorphonuclear cells and lymphocyte infiltration in the dermis. In contrast, the administration with either anti-CD80 or CD86 mAb 2 h before challenge partially inhibited CH reactions and a combination of both mAb did not improve the inhibitory effect. Although Langerhans cells (LC) expressing MHC class II were observed in both the epidermis and dermis 24 h after primary sensitization, CD86(+) LC were observed only in the subepidermal regions and CD80-bearing cells were not detected. Dendritic cells (DC) expressing both CD80 and CD86 were preferentially observed in the T cell areas of draining lymph nodes 24 h after challenge. The administration of anti-CD86 mAb in the induction phase prominently reduced the up-regulation of CD80 and CD86 on DC in the lymph nodes. The predominant role of CD86 was further supported by a marked reduction in proliferation of lymph node T cells against the sensitized hapten after the anti-CD86 treatment. These results suggest that CD86 plays a critical role in the initiation of primary immune responses in the skin, while CD80 and CD86 are not essential in the effector phase of CH reactions.
authors.affiliation: Department of Immunology, Juntendo University School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo 113, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Nuriya
authors.forename: H
authors.initials: H
authors.lastname: Yagita
authors.forename: K
authors.initials: K
authors.lastname: Okumura
authors.forename: M
authors.initials: M
authors.lastname: Azuma
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D015703:Antigens, CD / Q000276:immunology*; D018122:B7-1 Antigen / Q000276:immunology*; D051940:B7-2 Antigen; D003877:Dermatitis, Contact / Q000209:etiology* / Q000276:immunology*; D005260:Female; D006241:Haptens / Q000276:immunology; D007114:Immunization / Q000379:methods; D007801:Langerhans Cells / Q000276:immunology; D008213:Lymphocyte Activation / Q000276:immunology; D008562:Membrane Glycoproteins / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D015398:Signal Transduction / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D018122:B7-1 Antigen; D051940:B7-2 Antigen; C497965:Cd86 protein, mouse; D006241:Haptens; D008562:Membrane Glycoproteins