title: Identification and characterization of novel dermal Thy-1 antigen-bearing dendritic cells in murine skin.
abstract: Using immunofluorescence on dermal sheets of mouse ear, we identified novel Thy-1+ dermal dendritic cells in murine skin. These cells are resistant to topical corticosteroid treatment. The number of these dermal Thy-1+ dendritic cells is not altered with aging of the mice or with 3 d of culture. Dermal Thy-1+ dendritic cells have melanosomes in their cytoplasm; hence, phagocytic activity is likely to be present. Double immunofluorescence revealed that most of these dermal Thy-1+ dendritic cells express CD45. Furthermore, conventional immunofluorescence and confocal laser scanning microscopic findings showed that most of these Thy-1+ dermal dendritic cells express gamma delta and V gamma 3 T-cell receptors. The relationship between these dermal Thy-1+ dendritic cells and dendritic epidermal T cells is discussed.
authors.affiliation: Department of Dermatology, Yamanashi Medical University and The University of Tokyo, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Tamaki
authors.forename: N
authors.initials: N
authors.lastname: Yasaka
authors.forename: C H
authors.initials: CH
authors.lastname: Chang
authors.forename: N
authors.initials: N
authors.lastname: Ohtake
authors.forename: A
authors.initials: A
authors.lastname: Saitoh
authors.forename: K
authors.initials: K
authors.lastname: Nakamura
authors.forename: M
authors.initials: M
authors.lastname: Furue
mesh_terms: D000818:Animals; D003713:Dendritic Cells / Q000276:immunology* / Q000648:ultrastructure; D005260:Female; D005455:Fluorescent Antibody Technique; D007150:Immunohistochemistry; D051379:Mice; D008806:Mice, Inbred AKR; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D018613:Microscopy, Confocal; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000378:metabolism; D012867:Skin / Q000166:cytology / Q000276:immunology*; D018800:Thy-1 Antigens / Q000378:metabolism*
chemical_list: D016692:Receptors, Antigen, T-Cell, gamma-delta; D018800:Thy-1 Antigens