title: Structure of the human myelin/oligodendrocyte glycoprotein gene and multiple alternative spliced isoforms.
abstract: Myelin/oligodendrocyte glycoprotein (MOG), a specific component of the central nervous system localized on the outermost lamellae of mature myelin, is a member of the immunoglobulin superfamily. We report here the organization of the human MOG gene, which spans approximately 17 kb, and the characterization of six MOG mRNA splicing variants. The intron/exon structure of the human MOG gene confirmed the splicing pattern, supporting the hypothesis that mRNA isoforms could arise by alternative splicing of a single gene. In addition to the eight exons coding for the major. MOG isoform, the human MOG gene also contains, in the 3' region, a previously unknown alternatively spliced coding exon, VIA. Alternative utilization of two acceptor splicing sites for exon VIII could produce two different C-termini. The nucleotide sequences presented here may be a useful tool to study further possible involvement of the MOG gene in hereditary neurological disorders.
authors.affiliation: Unité 1488 du Centre National de la Recherche Scientifique, Laboratoire de Neurogénétique Moléculaire, Université de Paris VI, France.
authors.forename: D
authors.initials: D
authors.lastname: Pham-Dinh
authors.forename: B
authors.initials: B
authors.lastname: Della Gaspera
authors.forename: N
authors.initials: N
authors.lastname: Kerlero de Rosbo
authors.forename: A
authors.initials: A
authors.lastname: Dautigny
mesh_terms: D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D001921:Brain / Q000378:metabolism*; D005091:Exons; D014644:Genetic Variation; D006801:Humans; D007438:Introns; D008969:Molecular Sequence Data; D009185:Myelin Proteins; D018992:Myelin-Associated Glycoprotein / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D063308:Myelin-Oligodendrocyte Glycoprotein; D009419:Nerve Tissue Proteins / Q000096:biosynthesis / Q000235:genetics; D017433:Protein Structure, Secondary; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D015183:Restriction Mapping; D014158:Transcription, Genetic
chemical_list: C571326:MOG protein, human; D009185:Myelin Proteins; D018992:Myelin-Associated Glycoprotein; D063308:Myelin-Oligodendrocyte Glycoprotein; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger