title: Detailed description of four YAC contigs representing 17 Mb of chromosome 4 of Arabidopsis thaliana ecotype Columbia.
abstract: The detailed arrangement of 563 YAC clones comprising four contigs covering approximately 17 Mbp of chromosome 4 is presented. YAC clones were positioned relative to each other and to markers by taking into account marker and end fragment hybridization data and the sizes of all YAC clones. This analysis made it possible to estimate physical distances between the majority of chromosome 4 markers. It also identified a relatively large number of YAC clones containing chimaeric inserts. The YAC contig map of the Columbia ecotype presents an important resource for map-based cloning experiments, rapid mapping of DNA sequences and large-scale genomic sequencing programs.
authors.affiliation: Department of Molecular Genetics, John Innes Centre, Colney, UK.
authors.forename: R
authors.initials: R
authors.lastname: Schmidt
authors.forename: J
authors.initials: J
authors.lastname: West
authors.forename: G
authors.initials: G
authors.lastname: Cnops
authors.forename: K
authors.initials: K
authors.lastname: Love
authors.forename: A
authors.initials: A
authors.lastname: Balestrazzi
authors.forename: C
authors.initials: C
authors.lastname: Dean
mesh_terms: D017360:Arabidopsis / Q000235:genetics*; D001483:Base Sequence; D002678:Chimera; D002874:Chromosome Mapping; D016386:Chromosome Walking; D018244:Chromosomes, Artificial, Yeast; D015825:Chromosomes, Fungal; D003001:Cloning, Molecular; D017931:DNA Primers; D015342:DNA Probes; D004271:DNA, Fungal / Q000737:chemistry; D015723:Gene Library; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012091:Repetitive Sequences, Nucleic Acid
chemical_list: D017931:DNA Primers; D015342:DNA Probes; D004271:DNA, Fungal