Study of second messenger levels and of sugar catabolism enzyme activities in transformed cells resistant to ionizing radiations.
We measured the level of second messengers, the activity of carbohydrate metabolism enzymes, and the resistance to ionizing radiations in normal 32D hematopoietic cells, in v-erbB transformants and in spontaneous transformants. v-erbB and spontaneous transformants were resistant to radiations as compared with their normal counterpart. The second messenger diacylglycerol was elevated in radioresistant clones. Only v-erbB transformants showed increase of the activities of enolase and glucose-6-phosphate dehydrogenase. v-erbB-transformed NIH/3T3 cells, selected as control, showed identical correlation between radioresistance, increase of diacylglycerol, and of enolase and glucose-6-phosphate dehydrogenase activity. These results indicate that increase of diacylglycerol is correlated with resistance to the killing effect of ionizing radiations and could be proposed as a marker of radioresponse.
Pacini
S
S
Institute of General Pathology, University of Firenze, Italy.
Ruggiero
M
M
Casamassima
F
F
Santucci
M A
MA
Milano
F
F
Ranaldi
F
F
Vanni
S
S
Giachetti
E
E
D016475:3T3 Cells / Q000378:metabolism / Q000528:radiation effects; D000818:Animals; D050260:Carbohydrate Metabolism; D002460:Cell Line; D002461:Cell Line, Transformed; D002471:Cell Transformation, Neoplastic / Q000528:radiation effects*; D004075:Diglycerides / Q000378:metabolism; D004307:Dose-Response Relationship, Radiation; D018773:Genes, erbB-1 / Q000235:genetics; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism*; D006412:Hematopoietic Stem Cells / Q000201:enzymology / Q000378:metabolism* / Q000528:radiation effects*; D007295:Inositol Phosphates / Q000378:metabolism; D007377:Interleukin-3 / Q000378:metabolism / Q000494:pharmacology; D051379:Mice; D010751:Phosphopyruvate Hydratase / Q000378:metabolism*; D010767:Phosphorylcholine / Q000378:metabolism; D011836:Radiation Tolerance; D014162:Transfection
D004075:Diglycerides; D007295:Inositol Phosphates; D007377:Interleukin-3; D010767:Phosphorylcholine; D005954:Glucosephosphate Dehydrogenase; D010751:Phosphopyruvate Hydratase