title: Intestinal neuromuscular function after preservation and transplantation.
abstract: While it is well known that prolonged preservation of the intestinal graft causes severe mucosal damage after transplantation, little is known about the effect on neuromuscular function. The entire small intestine of adult hound dogs was flushed and preserved with cold lactated Ringer's solution and autotransplanted either immediately (n = 6) or after 24 hr (n = 6). Animals undergoing sham operation (n = 4) were used as a control. Fasting motility and the response of the intestinal smooth muscle and enteric nerves to bethanechol (100 microg/kg/0.5 hr, iv) and cisapride (0.5 mg/kg, iv) were determined by a multiple strain gauge method on Postoperative Days 2,4,7,14,21, and 28. Compared to the control, immediately transplanted grafts and those preserved for 24 hr developed delayed reappearance of migrating myoelectric complexes (MMC), hypercontractile activity, and reduced response to bethanechol and cisapride administration. Animals in the preservation group developed more abnormal fasting motility after transplantation, but responses to bethanechol and cisapride stimulation were not markedly different from those of the immediate group. The reappearance of MMC occurred 3 weeks postoperatively in the preservation group compared to 2 days in the immediate group. The results of our study indicate that intestinal dysmotility is augmented in prolonged-preservation grafts compared to those with brief preservation. The dysmotility was transient and normalized 3 to 4 weeks after surgery. Preservation and reperfusion injury to the neuromuscular system of intestinal grafts are reversible and are attenuated by simple hypothermia.
authors.affiliation: Pittsburgh Transplantation Institute, University of Pittsburgh, School of Medicine, Pittsburgh, PA 15213, USA.
authors.forename: N
authors.initials: N
authors.lastname: Hamada
authors.forename: W R
authors.initials: WR
authors.lastname: Hutson
authors.forename: K
authors.initials: K
authors.lastname: Nakada
authors.forename: A
authors.initials: A
authors.lastname: Ikoma
authors.forename: T
authors.initials: T
authors.lastname: Suzuki
authors.forename: Y
authors.initials: Y
authors.lastname: Zhu
authors.forename: T E
authors.initials: TE
authors.lastname: Starzi
authors.forename: S
authors.initials: S
authors.lastname: Todo
mesh_terms: D000818:Animals; D018723:Bethanechol / Q000494:pharmacology; D020117:Cisapride; D004285:Dogs; D005215:Fasting; D005260:Female; D007422:Intestines / Q000294:innervation* / Q000637:transplantation*; D008297:Male; D016065:Myoelectric Complex, Migrating / Q000187:drug effects; D009469:Neuromuscular Junction / Q000502:physiology*; D009926:Organ Preservation; D010880:Piperidines / Q000494:pharmacology; D013997:Time Factors; D014182:Transplantation, Autologous
chemical_list: D010880:Piperidines; D018723:Bethanechol; D020117:Cisapride