An evaluation of the genotoxic properties of some chosen dyes using the micronucleus test in vivo.
The frequency of micronucleated polychromatic erythrocytes and the polychromatic to normochromatic erythrocyte ratio was studied in BalbC mice treated with four azo dyes: Direct Blue 74, Direct Blue 296, Direct Blue 297 and Direct Green 98 at two (40% and 80% LD50/kg body weight) concentrations. None of the studied compounds revealed a genotoxic activity in the micronucleus test. However, it was found that two dyes, direct Blue 297 at doses 40% and 80% LD50 and Direct Green 98 at dose 80% LD50, cause a significant decrease in the ration of polychromatic to normochromatic erythrocytes in bone marrow of mice, which means that at the doses specified above they can affect the proliferation of the blood cells.
Przybojewska
B
B
Department of Toxicological Biochemistry, Institute of Occupational Medicine, Lodz, Poland.
D000818:Animals; D004396:Coloring Agents / Q000633:toxicity*; D005260:Female; D007928:Lethal Dose 50; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D015162:Micronucleus Tests; D016685:Mitomycin / Q000633:toxicity; D009153:Mutagens / Q000633:toxicity*
D004396:Coloring Agents; D009153:Mutagens; D016685:Mitomycin