Intracellular free Ca2+ dynamic changes to histamine are reduced in cystic fibrosis human tracheal gland cells.
This study documents a difference between cystic fibrosis human (CF-HTG) and normal human (HTG) tracheal gland cells: the ability of histamine to induce an increase of intracellular free calcium concentration [Ca2+]i was abnormally reduced in CF-HTG cells. The magnitude of the [Ca2+]i peak rise in response to histamine is smaller in CF-HTG cells than in HTG cells, and the percentage of CF-HTG cells that increase [Ca2+]i is decreased compared with HTG cells. In contrast to histamine, the human neutrophil elastase (HNE) stimulation of both CF-HTG and HTG cells generated [Ca2+]i asynchronous oscillations and the magnitude of the peak [Ca2+]i response as well as the percentage of responding cells were similar for both groups. By videomicroscopy observations, the secretory response (exocytosis of secretion granules) of CF-HTG cells occurred with HNE, but not with histamine, thus suggesting that [Ca2+]i asynchronous oscillations may be linked to the exocytosis process in human tracheal gland cells.
Jacquot
J
J
INSERM U. 314, Université de Reims, France.
Maizières
M
M
Spilmont
C
C
Millot
J M
JM
Sébille
S
S
Merten
M
M
Kammouni
W
W
Manfait
M
M
D000001:Calcimycin / Q000031:analogs & derivatives / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D003550:Cystic Fibrosis / Q000378:metabolism* / Q000473:pathology; D006632:Histamine / Q000494:pharmacology*; D006801:Humans; D007476:Ionophores / Q000494:pharmacology; D019272:Leukocyte Elastase; D010196:Pancreatic Elastase / Q000494:pharmacology; D014132:Trachea / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*
D007476:Ionophores; D000001:Calcimycin; C033170:4-bromo-A-23187; D006632:Histamine; D010196:Pancreatic Elastase; D019272:Leukocyte Elastase; D002118:Calcium