Identification of a cDNA encoding a thiazide-sensitive sodium-chloride cotransporter from the human and its mRNA expression in various tissues.
We report here the identification of a cDNA encoding a human thiazide-sensitive sodium-chloride cotransporter (hTSC) using a PCR-based method. The homology of the hTSC with rat TSC (rTSC) and rat bumetanide-sensitive sodium-potassium-chloride cotransporter (rBSC) was 86% and 64%, respectively, at the nucleotide level, and 92% and 61%, respectively, at the amino acid level. Using fluorescence in situ hybridization (FISH), the hTSC gene has been mapped to chromosome 16q13. Northern blot analysis using polyA+RNA from various human tissues, revealed a major 4.5 kb transcript and a minor 6.5 kb specifically in the kidney, but low level of expression was also observed in small intestine, placenta, prostate, colon, and spleen.
Chang
H
H
Fourth Department of Internal Medicine, Faculty of Medicine, Universityof Tokyo, Japan. hohang-tky@umin.u-tokyo.ac.jp
Tashiro
K
K
Hirai
M
M
Ikeda
K
K
Kurokawa
K
K
Fujita
T
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001581:Benzothiadiazines; D002352:Carrier Proteins / Q000096:biosynthesis* / Q000737:chemistry* / Q000235:genetics; D002874:Chromosome Mapping; D002885:Chromosomes, Human, Pair 16; D003001:Cloning, Molecular; D003106:Colon / Q000378:metabolism; D017931:DNA Primers; D018076:DNA, Complementary; D004232:Diuretics; D005260:Female; D005432:Flounder; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007421:Intestine, Small / Q000378:metabolism; D007668:Kidney / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010920:Placenta / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D011467:Prostate / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D011955:Receptors, Drug / Q000096:biosynthesis* / Q000737:chemistry* / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D049993:Sodium Chloride Symporter Inhibitors / Q000378:metabolism; D049992:Sodium Chloride Symporters; D064486:Solute Carrier Family 12, Member 3; D013154:Spleen / Q000378:metabolism; D027981:Symporters; D014158:Transcription, Genetic
D001581:Benzothiadiazines; D002352:Carrier Proteins; D017931:DNA Primers; D018076:DNA, Complementary; D004232:Diuretics; D012333:RNA, Messenger; D011955:Receptors, Drug; C578874:SLC12A3 protein, human; C578876:Slc12a3 protein, rat; D049993:Sodium Chloride Symporter Inhibitors; D049992:Sodium Chloride Symporters; D064486:Solute Carrier Family 12, Member 3; D027981:Symporters; C064415:thiazide receptor