title: Improved rapid method for the isolation, purification and identification of collagen glycosides.
abstract: An updated method for obtaining purified glucosylgalactosyl hydroxylysine (Glc-Gal-Hyl) is presented. Marine sponge is subjected to alkaline hydrolysis followed by ion-exchange chromatography and gel filtration. Identification is confirmed by consecutive mild acid hydrolysis of the extract to produce galactosyl hydroxylysine (Gal-Hyl) and hydroxylysine (Hyl). High-performance liquid chromatography (HPCL) with a sodium acetate-acetonitrile buffer and dabsyl chloride as the derivatizing agent demonstrated the purity of the extract and its hydrolyzates. Glc-Gal-Hyl is not commercially available but this easy and efficient method can provide large amounts of it for collagen metabolism studies.
authors.affiliation: Collagen Research Laboratory, Houston, TX 77030, USA.
authors.forename: H
authors.initials: H
authors.lastname: Garza
authors.forename: N
authors.initials: N
authors.lastname: Bennett
authors.forename: G P
authors.initials: GP
authors.lastname: Rodriguez
mesh_terms: D000818:Animals; D002240:Carbohydrate Sequence; D003094:Collagen / Q000737:chemistry*; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D011161:Porifera
chemical_list: D006027:Glycosides; D003094:Collagen