title: The role of complement in the acute inflammatory process in the skin and in host-parasite interaction in hamsters inoculated with Leishmania (Leishmania) chagasi.
abstract: Tecidual reaction at the inoculation site of L. (L.)chagasi promastigotes in hamsters depleted and non-depleted of complement was studied within 2, 6, 12, 24, 48 and 72 hours of infection. The inflammatory reaction was characterized by early predominance of polymorphonuclear cells (PMN) at 2, 6 and 12 hours of infection, mixed infiltrate of PMN and mononuclear cells (MN) at 24 hours, followed by predominance of MN at 48 and 72 hours after infection. The group depleted of complement showed a higher number of PMN at 2 hours and lower numbers of MN at 72 hours after infection (P < 0.0001). In the depleted group the phagocytosis by PMN was lower at 2 and 24 hours and by MN was lower at 24, 48 and 72 hours after infection. Electron microscopy showed extracellular intact and degenerated parasites, and lysed intracellular parasites, in PMN; and, rarely, preserved intracellular parasites in MN at 2, 6 and 12 hours after infection. The groups examined at 24, 48 and 72 hours of infection showed only cellular and parasite debris in mononuclear inflammatory cells. C3b deposits were detected by immunofluorescence in the interstitium and in the cytoplasm of inflammatory cells in non-depleted group at 2, 6 and 12 hours of infection. No immunoglobulin was detected in either group. Visceralization was detected 240 days after infection. The complement system has an important role in the inflammatory reaction and phagocytosis. The ultrastructural findings showed that the escape of the parasite probably occurs soon after inoculation.
authors.affiliation: Department of Pathology, University of São Paulo, Brazil.
authors.forename: M D
authors.initials: MD
authors.lastname: Laurenti
authors.forename: C E
authors.initials: CE
authors.lastname: Corbett
authors.forename: M N
authors.initials: MN
authors.lastname: Sotto
authors.forename: I L
authors.initials: IL
authors.lastname: Sinhorini
authors.forename: H
authors.initials: H
authors.lastname: Goto
mesh_terms: D000208:Acute Disease; D000818:Animals; D003176:Complement C3 / Q000276:immunology*; D006224:Cricetinae; D006790:Host-Parasite Interactions / Q000276:immunology; D018314:Leishmania infantum / Q000502:physiology* / Q000648:ultrastructure; D007898:Leishmaniasis, Visceral / Q000276:immunology* / Q000469:parasitology / Q000473:pathology; D008264:Macrophages / Q000276:immunology; D008297:Male; D008647:Mesocricetus; D008854:Microscopy, Electron; D009504:Neutrophils / Q000276:immunology; D010587:Phagocytosis; D012867:Skin / Q000276:immunology*; D012876:Skin Diseases, Parasitic / Q000276:immunology* / Q000469:parasitology / Q000473:pathology; D013997:Time Factors
chemical_list: D003176:Complement C3