Molecular cloning and chromosomal mapping of the mouse gene encoding cyclin-dependent kinase 5 regulatory subunit p35.
A neural-specific activating subunit, p35, of cyclin-dependent kinase 5 (Cdk5) was recently reported to differ from other mammalian cyclins, suggesting a new type of regulatory subunit for Cdk activity. The mouse gene encoding p35, Cdk5r, was isolated from a mouse 129/SvJ genomic library, and the genomic structure of Cdk5r was characterized. The most notable features of Cdk5r are the absence of introns in the amino acid coding region and the high homology of amino acid sequence among species. The 5'-flanking region of Cdk5r contained no canonical TATA or CAAT box but had several putative promoter elements, including Sp1, AP2, MRE, and NGFIA. The mouse Cdk5r transcript was detected only in the brain by Northern blot analysis. Mouse Cdk5r was mapped to a position on mouse chromosome 11.
Ohshima
T
T
Developmental and Metabolic Neurology Branch, National Institutes of Health, Bethesda, Maryland, 20892-4326, USA.
Kozak
C A
CA
Nagle
J W
JW
Pant
H C
HC
Brady
R O
RO
Kulkarni
A B
AB
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D003433:Crosses, Genetic; D051360:Cyclin-Dependent Kinase 5; D018844:Cyclin-Dependent Kinases; D017931:DNA Primers; D018076:DNA, Complementary; D005819:Genetic Markers; D006801:Humans; D046911:Macromolecular Substances; D051379:Mice / Q000235:genetics*; D008969:Molecular Sequence Data; D009115:Muridae; D016133:Polymerase Chain Reaction; D017346:Protein Serine-Threonine Kinases / Q000096:biosynthesis / Q000235:genetics*; D011995:Recombination, Genetic
D017931:DNA Primers; D018076:DNA, Complementary; D005819:Genetic Markers; D046911:Macromolecular Substances; D051360:Cyclin-Dependent Kinase 5; D017346:Protein Serine-Threonine Kinases; C495905:CDK5 protein, human; C495906:Cdk5 protein, mouse; D018844:Cyclin-Dependent Kinases