Crystal structure of a human TATA box-binding protein/TATA element complex.
The TATA box-binding protein (TBP) is required by all three eukaryotic RNA polymerases for correct initiation of transcription of ribosomal, messenger, small nuclear, and transfer RNAs. The cocrystal structure of the C-terminal/core region of human TBP complexed with the TATA element of the adenovirus major late promoter has been determined at 1.9 angstroms resolution. Structural and functional analyses of the protein-DNA complex are presented, with a detailed comparison to our 1.9-angstroms resolution structure of Arabidopsis thaliana TBP2 bound to the same TATA box.
Nikolov
D B
DB
Laboratory of Molecular Biophysics, The Rockefeller University, NY 10021, USA.
Chen
H
H
Halay
E D
ED
Hoffman
A
A
Roeder
R G
RG
Burley
S K
SK
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000737:chemistry; D001483:Base Sequence; D017124:Conserved Sequence; D018360:Crystallography, X-Ray; D004247:DNA / Q000737:chemistry / Q000235:genetics; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008958:Models, Molecular; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009690:Nucleic Acid Conformation; D010940:Plant Proteins / Q000737:chemistry; D011487:Protein Conformation; D016385:TATA Box; D035181:TATA-Box Binding Protein; D014157:Transcription Factors / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
D004268:DNA-Binding Proteins; D010940:Plant Proteins; D035181:TATA-Box Binding Protein; D014157:Transcription Factors; D004247:DNA