title: Regional assignment of human ESTs by whole-genome radiation hybrid mapping.
abstract: The UK HGMP Resource Centre's collection of human partial cDNA sequences (ESTs) have been examined for suitability for mapping by PCR on a panel of somatic cell hybrids. The chromosomal assignments of 92 ESTs were determined with a monochromosomal hybrid panel, and a subset of 45 were linked to genetic markers with a panel of whole-genome radiation hybrids (WG-RHs). These results demonstrate the potential of WG-RHs to construct a transcript map of the human genome.
authors.affiliation: HGMP Resource Centre, Hinxton, Cambridgeshire, UK.
authors.forename: P D
authors.initials: PD
authors.lastname: Hayes
authors.forename: K
authors.initials: K
authors.lastname: Schmitt
authors.forename: H B
authors.initials: HB
authors.lastname: Jones
authors.forename: G
authors.initials: G
authors.lastname: Gyapay
authors.forename: J
authors.initials: J
authors.lastname: Weissenbach
authors.forename: P N
authors.initials: PN
authors.lastname: Goodfellow
mesh_terms: D001483:Base Sequence; D002874:Chromosome Mapping; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics; D016678:Genome; D006801:Humans; D006822:Hybrid Cells / Q000528:radiation effects; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D016324:Sequence Tagged Sites
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary