Application of anti-E1 monoclonal antibodies to the study of the pyruvate dehydrogenase complex.
It has been shown that monoclonal antibody (mAb) F7F10 raised against pyruvate dehydrogenase component (E1) of pigeon breast muscle pyruvate dehydrogenase complex (PDC) has no influence on the E1 activity, measured in the system with artificial oxidants. However it inhibited the full NAD+ and coenzyme A dependent activity of PDC. The competition of the F7F10 antibody with the E2 component of PDC for the binding with E1 was revealed by immunoenzymatic and kinetic analysis. It is suggested that F7F10 mAb interacts with an antigenic determinant, located in the immediate vicinity of or overlapping with the E1 region, responsible for the interaction with the E2 component of PDC.
Stafejeva
O A
OA
A.N. Bakh Institute of Biochemistry Russian Academy of Sciences, Moscow, Russian Federation.
Khailova
L S
LS
Katrukha
A G
AG
Bulargina
T V
TV
D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism / Q000494:pharmacology*; D001666:Binding Sites, Antibody; D001667:Binding, Competitive; D010856:Columbidae; D000939:Epitopes / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D007655:Ketoglutarate Dehydrogenase Complex / Q000187:drug effects / Q000276:immunology; D007700:Kinetics; D009132:Muscles / Q000201:enzymology; D009206:Myocardium / Q000201:enzymology; D009243:NAD / Q000378:metabolism; D011768:Pyruvate Dehydrogenase Complex / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D013835:Thiamine Pyrophosphate / Q000276:immunology / Q000378:metabolism
D000911:Antibodies, Monoclonal; D000939:Epitopes; D011768:Pyruvate Dehydrogenase Complex; D009243:NAD; D007655:Ketoglutarate Dehydrogenase Complex; D013835:Thiamine Pyrophosphate