[Effect of internal sequences in the Moloney murine leukemia virus genome on replication].
Construction and using of retrovirus vectors derived from the Moloney murine leukemia virus (MoMuLV) are described. These vectors, designated minimal vectors, contain the left and right long terminal repeats (LTRs), a binding site for proline tRNA, a polypurine tract (PPT), and a dominant marker for selective introduction of vectors into a packaging cell line, but lack the internal sequences of the virus genome. The experiments showed that the minimal vectors can be replicated and that their titer was approximately 1500-fold lower than that of wild-type vectors. The minimal vectors were shown to contain all the cis-acting sequences necessary for correct reverse transcription. One infectious virion. like wild-type viruses, produced only one provirus. Unlike the avian reticuloendotheliosis virus (REV), psi+ and psi(-)-genomes of MoMuLV did not compete for virion proteins in the psi2 packaging cell line. When an insert was introduced into a central part of the lTR U5 region, the titer of the minimal vector remained the same, while the titer of the wild-type vector decreased approximately 40-fold.
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D016475:3T3 Cells; D000818:Animals; D004261:DNA Replication / Q000235:genetics*; D005822:Genetic Vectors; D016679:Genome, Viral; D051379:Mice; D008979:Moloney murine leukemia virus / Q000235:genetics* / Q000472:pathogenicity; D012091:Repetitive Sequences, Nucleic Acid; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000378:metabolism; D014771:Virion / Q000235:genetics / Q000472:pathogenicity
D014764:Viral Proteins
Vliianie vnutrennikh posledovatel'nosteĭ genoma virusa leĭkemii mysheĭ moloni na replikatsiiu.