Epithelial tumour cells in bone marrow of patients with pancreatic carcinoma detected by immunocytochemical staining.
In the present study, epithelial cells in the bone marrow of 42 patients with pancreatic carcinoma were identified immunocytochemically with monoclonal antibodies (MAbs) CK2, KL1 and A45-B/B3 directed to epithelial cytokeratins (CK), using the alkaline phosphatase anti-alkaline phosphatase method. The specificity of the MAbs was demonstrated by negative staining of marrow from 25 non-carcinoma age-matched control patients. Analysis of bone marrow aspirates from cancer patients revealed CK-positive cells in 14 (58.3%) of 24 cancer patients treated with curative intent and 10 (55.6%) of 18 patients with extended disease. After a median follow-up of 15.6 months (range 3-31 months), 5 (35.7%) out of 14 patients who underwent complete surgical resection but had tumour cells in bone marrow presented with distant metastasis and 6 (42.9%) with local relapse as compared to none of 10 corresponding patients without such cells (P < 0.05). The described technique may help to identify patients with pancreatic cancer and potential high risk of early metastatic relapse. The results promise to be of important assistance in determining prognosis and consequences in therapy of early stage pancreatic cancer.
Thorban
S
S
Department of Surgery, Technische Universität München, Germany.
Roder
J D
JD
Pantel
K
K
Siewert
J R
JR
D000230:Adenocarcinoma / Q000175:diagnosis / Q000378:metabolism / Q000556:secondary*; D000911:Antibodies, Monoclonal; D019046:Bone Marrow Neoplasms / Q000175:diagnosis / Q000378:metabolism / Q000556:secondary*; D005500:Follow-Up Studies; D006801:Humans; D007124:Immunoenzyme Techniques; D007633:Keratins / Q000378:metabolism; D009363:Neoplasm Proteins / Q000378:metabolism; D009364:Neoplasm Recurrence, Local; D010190:Pancreatic Neoplasms / Q000473:pathology*; D011446:Prospective Studies
D000911:Antibodies, Monoclonal; D009363:Neoplasm Proteins; D007633:Keratins