Arachidonic acid activatable electrogenic H+ transport in the absence of cytochrome b558 in human T lymphocytes.
To test the suggested structural relationship between the electrogenic H+ transporting system and the NADPH oxidase of phagocytes, the existence of the enzyme and the transport process was investigated in human tonsillar T lymphocytes. It is shown that tonsillar T cells possess an arachidonic acid activatable, Cd(2+)- and Zn(2+)-sensitive electrogenic H+ efflux pathway with similar properties as reported earlier in various phagocytic cells. The presence of cytochrome b558, the membrane component of the oxidase, could not be detected in tonsillar T lymphocytes either by immunoblot or by flow cytometric analysis. It is suggested that the electrogenic H+ transporting pathway is structurally independent of the NADPH oxidase complex.
Káldi
K
K
Department of Physiology, Semmelweis University of Medicine, Budapest, Hungary.
Szászi
K
K
Koncz
G
G
Suszták
K
K
Ligeti
E
E
D016718:Arachidonic Acid / Q000494:pharmacology*; D002104:Cadmium / Q000494:pharmacology; D003573:Cytochrome b Group / Q000032:analysis / Q000172:deficiency / Q000378:metabolism*; D004024:Dicyclohexylcarbodiimide / Q000494:pharmacology; D005434:Flow Cytometry; D006098:Granulocytes / Q000276:immunology / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D009637:Masoprocol / Q000494:pharmacology; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism; D019255:NADPH Oxidases; D014066:Palatine Tonsil; D006180:Proton-Translocating ATPases / Q000187:drug effects / Q000378:metabolism*; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D015032:Zinc / Q000494:pharmacology
D003573:Cytochrome b Group; D002104:Cadmium; D016718:Arachidonic Acid; D004024:Dicyclohexylcarbodiimide; D009637:Masoprocol; C036888:cytochrome b558; D009247:NADH, NADPH Oxidoreductases; D019255:NADPH Oxidases; D006180:Proton-Translocating ATPases; D015032:Zinc