Gene transfer and amplification in CHO cells. Efficient methods for maximizing specific productivity and assessment of genetic consequences.
Wurm
F M
FM
Genentech Inc., Process Sciences, South San Francisco, California 94080, USA.
Johnson
A
A
Ryll
T
T
Köhne
C
C
Scherthan
H
H
Glaab
F
F
Lie
Y S
YS
Petropoulos
C J
CJ
Arathoon
W R
WR
D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D007074:Immunoglobulin G / Q000096:biosynthesis; D008727:Methotrexate / Q000494:pharmacology; D018124:Receptors, Tumor Necrosis Factor / Q000096:biosynthesis / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D011994:Recombinant Proteins / Q000096:biosynthesis*; D012190:Retroviridae; D013762:Tetrahydrofolate Dehydrogenase / Q000096:biosynthesis / Q000235:genetics; D014162:Transfection / Q000379:methods*
D007074:Immunoglobulin G; D018124:Receptors, Tumor Necrosis Factor; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; D013762:Tetrahydrofolate Dehydrogenase; D008727:Methotrexate