title: Reduction of oxalate content of foods by the oxalate degrading bacterium, Eubacterium lentum WYH-1.
abstract: BACKGROUND
  Urinary oxalate may contribute far more than urinary calcium to the pathogenesis of urinary calculi. Urinary oxalate may be reduced by restricting the intake of foods high in oxalate. The oxalate content foods might be reduced by oxalate-degrading bacteria. The purpose of this experiment was to reduce the oxalate content of foods with an oxalate-degrading bacterium which was isolated from the feces of Japanese male.
  
  
  METHODS
  An artificial intestinal juice was prepared by modifying Rogosa medium. An infusion of black tea was prepared from a commercial tea bag. The oxalate-degrading bacteria used were Eubacterium lentum WYH-1 which were have isolated. To 5 ml of the above oxalate-containing artificial intestinal juice and infusion of black tea, 0.5 ml of the bacterial culture was added and incubated anaerobically at 37 degrees C. Oxalic acid in the supernatant of the culture medium was assayed by high-performance liquid chromatography.
  
  
  RESULTS
  In 24 hours, 1 x 10(6) cells/ml of Eubacterium lentum WYH-1 decomposed 100% of 1 mg/ml oxalate in the artificial intestinal juice. The oxalate in the black tea infusion (1 mg/mL) was also decomposed completely within 48 hours by 1 x 10(7) cells/mL of bacteria.
  
  
  CONCLUSIONS
  Eubacterium lentum WYH-1 was able to efficiently decompose the oxalate in foods.
authors.affiliation: Department of Urology, Teikyo University School of Medicine, Ichihara Hospital, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Ito
authors.forename: N
authors.initials: N
authors.lastname: Miura
authors.forename: M
authors.initials: M
authors.lastname: Masai
authors.forename: K
authors.initials: K
authors.lastname: Yamamoto
authors.forename: T
authors.initials: T
authors.lastname: Hara
mesh_terms: D002851:Chromatography, High Pressure Liquid; D005051:Eubacterium / Q000378:metabolism*; D005243:Feces / Q000382:microbiology; D005504:Food Analysis; D005516:Food Microbiology; D006801:Humans; D007419:Intestinal Secretions / Q000737:chemistry / Q000382:microbiology; D008297:Male; D010070:Oxalates / Q000032:analysis* / Q000652:urine*; D014545:Urinary Calculi / Q000178:diet therapy / Q000209:etiology / Q000382:microbiology*
chemical_list: D010070:Oxalates