title: Analysis of linear oligogalacturonic acids by negative-ion electrospray ionization mass spectrometry.
abstract: Linear oligogalacturonic acids (1,4-linked alpha-D-galacturonic acid oligomers), obtained by partial acid hydrolysis of orange polygalacturonides, were studied by negative-ion electrospray ionization mass spectrometry, without prior sample derivatization. After preparative separation using high-resolution anion-exchange chromatography, some fractions enriched in uronic acids were desalted, transferred into a methanol+water solution adjusted to pH 10, and directly submitted to electrospray ionization mass spectrometry in the negative-ion recording mode. Clear molecular mass assignments of the oligomers, covering a degree of polymerization between 4 and 7, were obtained.
authors.affiliation: Institut National Agronomique, Laboratoire de Chimie Analytique, Parix, France.
authors.forename: M
authors.initials: M
authors.lastname: Xie
authors.forename: D
authors.initials: D
authors.lastname: Giraud
authors.forename: Y
authors.initials: Y
authors.lastname: Bertheau
authors.forename: B
authors.initials: B
authors.lastname: Casetta
authors.forename: P
authors.initials: P
authors.lastname: Arpino
mesh_terms: D002240:Carbohydrate Sequence; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000032:analysis*; D010368:Pectins / Q000032:analysis*
chemical_list: D009844:Oligosaccharides; D010368:Pectins; C003181:polygalacturonic acid