Induction of endothelial adhesion molecules by rat cytomegalovirus in allogeneic lung transplantation in the rat.
Cytomegalovirus (CMV) infection is known to be a major risk factor for the development of chronic transplant rejection in heart and lung transplantation. A possible mechanism for the induction of lung transplant rejection by CMV infection is the inflammatory upregulation of adhesion ligand molecules by the viral infection leading to an increased endothelial-leucocyte interaction. To study this question, an experimental model was established in the rat using a rat cytomegalovirus (RCMV) infection and acute lung transplant rejection in left single lung transplantation. The distribution of RCMV, intercellular adhesion molecule-1 (ICAM-1) and its leucocyte receptor CD11a (LFA-1) were investigated by immunohistochemistry. The viral infection was observed in transplant lungs of infected hosts as early as day 11. The expression of ICAM-1 on endothelial cells was induced and enhanced by RCMV infection, and infiltration of CD11a-positive leucocytes found to be increased in infected recipients. An acceleration of the rejection of the allografts by the hosts was found.
Steinhoff
G
G
Department of Cardiovascular Surgery, University of Kiel, Germany.
You
X M
XM
Steinmüller
C
C
Boeke
K
K
Stals
F S
FS
Bruggeman
C A
CA
Haverich
A
A
D000818:Animals; D018845:CD11 Antigens / Q000032:analysis; D015815:Cell Adhesion Molecules / Q000032:analysis*; D003586:Cytomegalovirus Infections / Q000209:etiology / Q000276:immunology* / Q000473:pathology; D004195:Disease Models, Animal; D004730:Endothelium, Vascular / Q000276:immunology / Q000473:pathology; D006085:Graft Survival; D007150:Immunohistochemistry; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis; D016040:Lung Transplantation / Q000009:adverse effects* / Q000276:immunology; D008297:Male; D051381:Rats; D011914:Rats, Inbred BN; D011917:Rats, Inbred Lew; D016028:Receptors, Leukocyte-Adhesion / Q000032:analysis*; D014181:Transplantation Immunology / Q000276:immunology*; D014184:Transplantation, Homologous
D018845:CD11 Antigens; D015815:Cell Adhesion Molecules; D016028:Receptors, Leukocyte-Adhesion; D018799:Intercellular Adhesion Molecule-1