title: The effect of immune sera from hamsters immunized with sandfly gut and whole body extract antigens on the fecundity and mortality of Phlebotomus duboscqi (Diptera: Psychodidae).
abstract: Phlebotomus duboscqi were fed on hamsters previously immunized with different concentrations of homogenized crude sandfly gut antigen and supernatant obtained from whole body extract. The humoral response in the rodents was quantified at different times post-immunization by the enzyme-linked immunosorbent assay. Sandflies were fed on either immunized or saline control hamsters and the effect of the blood meals on sandfly feeding, survival and fecundity was investigated. The humoral response in immunized hamsters as measured by the presence of P. duboscqi-specific IgG antibodies was significantly greater (P < 0.05) as compared to the controls. This difference was noted in sera collected on 15 and 25 days post-immunization. Sandflies fed on immunized hamsters had a significantly higher mortality (P < 0.05) and decreased egg production (P < 0.05) than those fed on unimmunized control hamsters.
authors.affiliation: Department of Zoology, Moi University, Eldoret, Kenya.
authors.forename: P
authors.initials: P
authors.lastname: Ingonga
authors.forename: P A
authors.initials: PA
authors.lastname: Mbati
authors.forename: C O
authors.initials: CO
authors.lastname: Anjili
authors.forename: A
authors.initials: A
authors.lastname: Mutani
authors.forename: B
authors.initials: B
authors.lastname: Wishitemi
authors.forename: S
authors.initials: S
authors.lastname: Odongo
authors.forename: L L
authors.initials: LL
authors.lastname: Robert
authors.forename: J I
authors.initials: JI
authors.lastname: Githure
mesh_terms: D000818:Animals; D000941:Antigens / Q000008:administration & dosage* / Q000097:blood / Q000276:immunology; D006224:Cricetinae; D004797:Enzyme-Linked Immunosorbent Assay; D005247:Feeding Behavior; D005260:Female; D005298:Fertility / Q000276:immunology; D007106:Immune Sera / Q000008:administration & dosage / Q000494:pharmacology*; D007116:Immunization, Passive; D007074:Immunoglobulin G / Q000097:blood; D008297:Male; D008647:Mesocricetus; D010691:Phlebotomus / Q000254:growth & development / Q000276:immunology*; D016019:Survival Analysis
chemical_list: D000941:Antigens; D007106:Immune Sera; D007074:Immunoglobulin G