title: A cleavage-site-directed inhibitor of interleukin-1 beta-converting enzyme-like proteases inhibits apoptosis in primary cultures of rat hepatocytes.
abstract: Apoptosis induced in primary hepatocytes by transforming growth factor beta1 and staurosporine produced chromatin condensation, DNA cleavage is detected by in situ end-labelling, field inversion and conventional gel electrophoresis, and cell detachment. These effects were abolished by benzyloxycarbonyl-valinylalanylaspartylfluoromethyl ketone, a cleavage-site-directed inhibitor of interleukin-1beta-converting enzyme-like proteases, and this finding suggests that these enzymes are involved in liver apoptosis.
authors.affiliation: MRC Toxicology Unit, Leicester, U.K.
authors.forename: K
authors.initials: K
authors.lastname: Cain
authors.forename: S H
authors.initials: SH
authors.lastname: Inayat-Hussain
authors.forename: C
authors.initials: C
authors.lastname: Couet
authors.forename: G M
authors.initials: GM
authors.lastname: Cohen
mesh_terms: D000470:Alkaloids / Q000494:pharmacology; D000590:Amino Acid Chloromethyl Ketones / Q000494:pharmacology*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D020170:Caspase 1; D002467:Cell Nucleus / Q000187:drug effects; D002478:Cells, Cultured; D002843:Chromatin / Q000187:drug effects / Q000378:metabolism; D003546:Cysteine Endopeptidases / Q000378:metabolism*; D015853:Cysteine Proteinase Inhibitors / Q000494:pharmacology*; D004247:DNA / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D008099:Liver / Q000166:cytology* / Q000187:drug effects; D008297:Male; D009707:Nucleosomes / Q000187:drug effects / Q000378:metabolism; D009842:Oligopeptides / Q000494:pharmacology; D011480:Protease Inhibitors / Q000494:pharmacology; D051381:Rats; D019311:Staurosporine; D016212:Transforming Growth Factor beta / Q000494:pharmacology
chemical_list: D000470:Alkaloids; D000590:Amino Acid Chloromethyl Ketones; D002843:Chromatin; D015853:Cysteine Proteinase Inhibitors; C098738:N-acetyl-tyrosyl-valyl-alanyl-aspartyl chloromethyl ketone; D009707:Nucleosomes; D009842:Oligopeptides; D011480:Protease Inhibitors; D016212:Transforming Growth Factor beta; C096713:benzyloxycarbonylvalyl-alanyl-aspartyl fluoromethyl ketone; C096429:L 709049; D004247:DNA; D003546:Cysteine Endopeptidases; D020170:Caspase 1; D019311:Staurosporine