title: A novel plasmid from Staphylococcus epidermidis specifying resistance to kanamycin, neomycin and tetracycline.
abstract: The naturally occurring plasmid pSTS7 from Staphylococcus epidermidis mediated resistance to tetracycline via a tetL gene and to kanamycin and neomycin via an aadD gene. Plasmid pSTS7 showed partial restriction map and sequence homology to the previously described tetracycline resistance plasmid pNS1981 from Bacillus subtilis and to the kanamycin/neomycin/bleomycin resistance plasmid pUB110 from S. aureus. Sequence analysis of the regions flanking the two resistance genes in pSTS7 led to the identification of a novel site for interplasmid recombination which could explain the derivation of pSTS7 from the incompatible pNS1981- and pUB110-like parental plasmids under tetracycline-selective pressure.
authors.affiliation: Institut für Kleintierforschung Celle/Merbitz der Bundesforschungsanstalt für Landwirtschaft Braunschweig-Völkenrode (FAL), Germany.
authors.forename: S
authors.initials: S
authors.lastname: Schwarz
authors.forename: P D
authors.initials: PD
authors.lastname: Gregory
authors.forename: C
authors.initials: C
authors.lastname: Werckenthin
authors.forename: S
authors.initials: S
authors.lastname: Curnock
authors.forename: K G
authors.initials: KG
authors.lastname: Dyke
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001412:Bacillus subtilis / Q000187:drug effects / Q000235:genetics; D001483:Base Sequence; D004269:DNA, Bacterial / Q000737:chemistry; D004352:Drug Resistance, Microbial / Q000235:genetics; D004818:Epidermitis, Exudative, of Swine / Q000382:microbiology; D007613:Kanamycin Resistance / Q000235:genetics*; D008969:Molecular Sequence Data; D009355:Neomycin / Q000494:pharmacology*; D011815:R Factors / Q000737:chemistry; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D013211:Staphylococcus aureus / Q000187:drug effects / Q000235:genetics; D013212:Staphylococcus epidermidis / Q000187:drug effects* / Q000235:genetics; D013552:Swine; D013753:Tetracycline Resistance / Q000235:genetics*; D014169:Transformation, Bacterial
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D009355:Neomycin