Selenium-containing compounds protect DNA from single-strand breaks caused by peroxynitrite.
Peroxynitrite (ONOO-/ONOOH) is a powerful oxidant that can induce mutations and cause single-strand breaks in plasmid supercoiled DNA. The selenium-containing compound ebselen rapidly reacts with peroxynitrite. Therefore, ebselen [2-phenyl-1,2-benzisoselenazol-3(2H)-one] and other selenium-containing molecules, selenomethionine and selenocystine, were studied as agents protecting DNA from single-strand breaks induced by peroxynitrite. Selenomethionine and selenocystine protected DNA from single-strand breaks more effectively than their sulfur analogs, methionine and cystine, and they also were more effective than glutathione or the hydroxyl radical scavenger mannitol. These results suggest that selenium-containing compounds can protect DNA from damage caused by peroxynitrite.
Roussyn
I
I
Insitut für Physiologische Chemie I, Heinrich-Universität Düsseldorf, Germany.
Briviba
K
K
Masumoto
H
H
Sies
H
H
D003553:Cystine / Q000031:analogs & derivatives* / Q000494:pharmacology; D004247:DNA / Q000737:chemistry / Q000187:drug effects*; D004249:DNA Damage; D008353:Mannitol / Q000494:pharmacology; D008715:Methionine / Q000494:pharmacology; D009566:Nitrates / Q000494:pharmacology*; D016566:Organoselenium Compounds / Q000494:pharmacology*; D010957:Plasmids / Q000187:drug effects*; D012645:Selenomethionine / Q000494:pharmacology*; D013438:Sulfhydryl Compounds / Q000494:pharmacology*
D009566:Nitrates; D016566:Organoselenium Compounds; D013438:Sulfhydryl Compounds; C009226:selenocystine; C039642:peroxynitric acid; D008353:Mannitol; D003553:Cystine; D004247:DNA; D012645:Selenomethionine; D008715:Methionine