title: Stimulation of erythrocyte membrane Mg(2+)-ATPase activity by glutathione S-conjugates.
abstract: Pig erythrocyte membrane Mg2+-ATPase activity was stimulated by various glutathione S-conjugates. For alkyl S-conjugates, the Km for the stimulation was lower, the more hydrophobic was the conjugate. 2,4-Dinitrophenyl-S-(N-acetyl)cysteine also stimulated the Mg2+-ATPase activity, suggesting a low specificity of the ¿glutathione S-conjugate pump¿. The Km values for the stimulation by 2,4-dinitrophenyl conjugates were lower than predictable on the basis of hydrophobicity which indicates a high affinity of the transporter for these conjugates.
authors.affiliation: Department of Molecular Biophysics, University of Lódź, Poland.
authors.forename: A
authors.initials: A
authors.lastname: Sokal
authors.forename: B
authors.initials: B
authors.lastname: Walkowiak
authors.forename: A
authors.initials: A
authors.lastname: Kałuzna
authors.forename: K
authors.initials: K
authors.lastname: Król
authors.forename: M
authors.initials: M
authors.lastname: Cieślak
authors.forename: B
authors.initials: B
authors.lastname: Rychlik
authors.forename: R
authors.initials: R
authors.lastname: Sychowski
authors.forename: G
authors.initials: G
authors.lastname: Bartosz
mesh_terms: D000111:Acetylcysteine / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000494:pharmacology; D000251:Adenosine Triphosphatases / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004910:Erythrocyte Membrane / Q000187:drug effects* / Q000201:enzymology* / Q000378:metabolism; D005978:Glutathione / Q000031:analogs & derivatives* / Q000494:pharmacology*; D007700:Kinetics; D008274:Magnesium / Q000378:metabolism*; D012995:Solubility; D013329:Structure-Activity Relationship; D013379:Substrate Specificity; D013552:Swine
chemical_list: C098267:2,4-dinitrophenyl-S-(N-acetylcysteine); C030190:S-(2,4-dinitrophenyl)glutathione; D000251:Adenosine Triphosphatases; D005978:Glutathione; D008274:Magnesium; D000111:Acetylcysteine