title: Hyperosmotic exposure alters total taurine quantity and cellular transport in rat astrocyte cultures.
abstract: Taurine content and cellular taurine transport were characterized in astrocytes from rat cerebral cortex after growth in isoosmotic or hyperosmotic culture conditions to investigate mechanisms of taurine accumulation during conditions of increased osmolality. Total taurine content of the culture dishes was significantly (P < 0.05) elevated after 8, 24, and 48 h of hyperosmotic exposure compared to cultures grown for the same period in isoosmotic (300 mOsm, control) conditions. Hyperosmotic medium elevated intracellular taurine (nmol/mg protein) levels by 29-108% over control cultures. Significant (P < 0.02) increases in carrier-mediated taurine uptake rates were observed in astrocytes exposed to 350, 400, and 450 mOsm culture medium for 24 h compared to control cultures at the same time point. The increase in uptake rate decreased to control values by 48 h in 450 mOsm treated cultures. The carrier-mediated transport binding constant for taurine uptake, Km, was not altered at any time after hyperosmotic treatment. Maximal velocity of uptake, V(max), increased by 70% and 36% after 24 h growth in 400 and 450 mOsm culture medium, respectively, compared to control cells at the same time. After 48 h of hyperosmotic exposure, V(max) returned to control values. The diffusional transport rate for taurine efflux, Kdiff, was not affected by hyperosmotic exposure at any time point. Taurine release rates were increased by over two-fold during the first 8 h of exposure to 450 mOsm medium compared with cells grown in control conditions. After 24 and 48 h hyperosmotic exposure, release rates decreased to 44-72% of the release from control cultures. These data indicate at least three mechanisms contribute to taurine accumulation in cultured cerebral astrocytes exposed to hyperosmotic conditions. These mechanisms are (i) an increased rate of taurine uptake from the extracellular space within 24 h, (ii) a decrease in net taurine efflux by 48 h, and (iii) an enhanced rate of taurine synthesis.
authors.affiliation: Department of Emergency Medicine, Wright State University School of Medicine, Dayton, OH 45429, USA. beetschj@kids.wustl.edu
authors.forename: J W
authors.initials: JW
authors.lastname: Beetsch
authors.forename: J E
authors.initials: JE
authors.lastname: Olson
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000502:physiology*; D001692:Biological Transport; D002478:Cells, Cultured; D003470:Culture Media; D005260:Female; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013654:Taurine / Q000378:metabolism*; D013997:Time Factors; D014882:Water-Electrolyte Balance
chemical_list: D003470:Culture Media; D013654:Taurine