Cloning and sequencing of the gene for Na+/H+ antiporter of Vibrio parahaemolyticus.
A gene encoding an Na+/H+ antiporter was cloned from vibrio parahaemolyticus into the plasmid pBR322 and expressed in Escherichia coli cells. The gene enabled mutant E. coli cells to grow in the presence of 0.2 M NaCl (or 10 mM LiCl). These cells were originally unable to grow under such conditions because of the lack of major Na+(Li+)/H+ antiporters. We detected Na+/H+ antiporter activity due to the gene in membrane vesicles. The gene was sequenced and the deduced amino acid sequence was found to be 72% identical to the NhaB Na+/H+ antiporter of E. coli.
Nozaki
K
K
Department of Microbiology, Faculty of Pharmaceutical Sciences, Okayama University, Japan.
Inaba
K
K
Kuroda
T
T
Tsuda
M
M
Tsuchiya
T
T
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D017923:Sodium-Hydrogen Exchangers / Q000235:genetics* / Q000378:metabolism; D014736:Vibrio parahaemolyticus / Q000235:genetics*
D004269:DNA, Bacterial; D017923:Sodium-Hydrogen Exchangers