Production of extracellular virulence factors by Pseudomonas aeruginosa isolates obtained from tracheal, urinary tract, and wound infections.
This study was conducted to determine the effect of the local environment within the host on the ability of P. aeruginosa to produce different extracellular virulence factors (elastase, phospholipase C, toxin A, and exoenzyme S). A total of 105 P. aeruginosa isolates was obtained from patients with tracheal, urinary tract, and wound infections. Quantitative analysis of the virulence factors was done by growing the isolates in vitro in different defined media. Single colonies of each isolate were inoculated from the primary isolation plates into the defined medium. All four virulence factors were produced by most isolates. However, depending on the location of their isolation, the isolates varied in the level of virulence factors they produced. High levels of elastase and phospholipase C were produced by most isolates obtained from trachea, urinary tract, and wounds. A significantly higher level of toxin A was produced by wound isolates, while a significantly higher level of exoenzyme S was produced by wound and urinary tract isolates. Some P. aeruginosa strains were frequently isolated from the same site of infection (persistent infection isolates). Comparative analysis of virulence factors produced by these isolates showed that, regardless of the isolation site, subsequent isolates produced higher levels of exoenzyme S. These results suggest that: (1) elastase, phospholipase C, toxin A, and exoenzyme S are produced by P. aeruginosa isolates from different sites of infection; (2) the production of higher levels of elastase and phospholipase C is important in all types of infections, while the production of toxin A and exoenzyme S is important in wound infection; (3) persistent infection with P.aeruginosa may enhance exoenzyme S production.
Hamood
A N
AN
Department of Microbiology and Immunology, Texas Tech University Health Sciences Center, Lubbock 79430, USA.
Griswold
J A
JA
Duhan
C M
CM
D036002:ADP Ribose Transferases; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D005098:Exotoxins / Q000032:analysis / Q000096:biosynthesis; D006801:Humans; D008666:Metalloendopeptidases / Q000032:analysis / Q000096:biosynthesis; D011065:Poly(ADP-ribose) Polymerases / Q000032:analysis / Q000096:biosynthesis; D011550:Pseudomonas aeruginosa / Q000378:metabolism / Q000472:pathogenicity*; D014132:Trachea / Q000382:microbiology*; D010738:Type C Phospholipases / Q000032:analysis / Q000096:biosynthesis; D014551:Urinary Tract / Q000382:microbiology*; D014774:Virulence; D037521:Virulence Factors; D014946:Wound Infection / Q000382:microbiology*; D000097668:Pseudomonas aeruginosa Exotoxin A
D036002:ADP Ribose Transferases; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D005098:Exotoxins; D008666:Metalloendopeptidases; D011065:Poly(ADP-ribose) Polymerases; D010738:Type C Phospholipases; D037521:Virulence Factors; D000097668:Pseudomonas aeruginosa Exotoxin A; C444820:exoenzyme S; C043859:pseudolysin, Pseudomonas aeruginosa