Nuclear matrix interactions within the sperm genome.
Analysis of the haploid-expressed human PRM1 --> PRM2 --> TNP2 genic domain has revealed two regions of attachment to the sperm nuclear matrix. These sperm nuclear matrix attachment regions delimit the DNase I-sensitive domain of this haploid-expressed locus. The domain is intermediately associated with but not attached to the nuclear matrix. DNase I-sensitive genes within the mature sperm nucleus, such as protamine 1, protamine 2, transition protein 2, alpha-globin, and beta-actin, display this intermediate affinity for the sperm nuclear matrix. This may denote their role in templating the male genome prior to fertilization, thus ensuring the formation of a viable male pronucleus during early embryonic development.
Kramer
J A
JA
Department of Obstetrics and Gynecology, Center for Molecular Medicine and Genetics, Wayne State University School of Medicine, Detroit, Michigan 48102, USA.
Krawetz
S A
SA
D001483:Base Sequence; D002868:Chromosomal Proteins, Non-Histone; D002874:Chromosome Mapping; D004247:DNA / Q000378:metabolism; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D015530:Nuclear Matrix / Q000378:metabolism; D009687:Nuclear Proteins / Q000235:genetics*; D011479:Protamines / Q000235:genetics*; D013094:Spermatozoa / Q000648:ultrastructure*
D002868:Chromosomal Proteins, Non-Histone; D009687:Nuclear Proteins; C082073:PRM1 protein, human; D011479:Protamines; C068621:protamine 2; C053886:spermatid transition proteins; D004247:DNA