Alteration of interleukin-1 alpha production and interleukin-1 alpha binding sites in mouse brain during rabies infection.
We have evaluated the effect of rabies virus infection on interleukin-1 alpha (IL-1 alpha) production and its receptors in mouse brain. Study of virus dissemination in the central nervous system (CNS) showed a massive infection of main brain structures from day 4 post infection (p.i.) up to the agony stage on day 6 p.i. At the same time, IL-1 alpha concentrations increased in cortical and hippocampal homogenates, whereas no change was detected in serum. In non-infected mice, IL-1 alpha binding sites were observed in the dentate gyrus, the cortex, the choroid plexus, the meninges and the anterior pituitary. During rabies virus infection, a striking decrease in IL-1 alpha binding sites was observed on day 4 p.i. with a complete disappearance on day 6 p.i., except in the pituitary gland where they remained at control level. In conclusion, concomitantly with the early rabid pathological signs, brain IL-1 alpha production and IL-1 alpha binding sites are specifically and significantly altered by brain viral proliferation. These results indicate that IL-1 alpha could be involved in the brain response to viral infection as a mediator and could participate in the genesis of the rabies pathogeny.
Marquette
C
C
Rabies Unit, Pasteur Institute, Paris, France.
Ceccaldi
P E
PE
Ban
E
E
Weber
P
P
Tsiang
H
H
Haour
F
F
D000818:Animals; D001921:Brain / Q000378:metabolism* / Q000473:pathology / Q000821:virology; D002540:Cerebral Cortex / Q000378:metabolism; D006624:Hippocampus / Q000378:metabolism; D007375:Interleukin-1 / Q000096:biosynthesis*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011818:Rabies / Q000378:metabolism*; D011820:Rabies virus / Q000502:physiology; D017472:Receptors, Interleukin-1 / Q000378:metabolism*; D014779:Virus Replication
D007375:Interleukin-1; D017472:Receptors, Interleukin-1