Effects of bradykinin on the intracellular calcium concentration of pancreatic acinar AR42J cells.
We examined the effects of bradykinin (BK) on the intracellular free calcium concentration ([Ca2+]i) in rat pancreatic acinar AR42J cells. BK induced a dose-dependent rise in [Ca+2]i in AR42J cells between the concentrations of 10(-12)M and 10(-7)M. The BK-evoked response was not affected by the presence of Co2+ or the absence of extracellular calcium. This response was suppressed by neomycin or the B2 antagonist, but not by the B1 antagonist. The response was also attenuated by treatment with dexamethasone. These results suggest that BK increases [Ca2+]i through the B2 receptors by promoting the phosphatidyl inositol turn-over and that, in the process of azaserine-induced undifferentiation, the pancreatic acinar cells strongly express the BK receptors.
Saito
Y
Y
Department of Laboratory Medicine, School of Medicine, Gunma University, Maebashi, Japan.
Kato
M
M
Kobayashi
I
I
Tatemoto
K
K
D000818:Animals; D001920:Bradykinin / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D065168:Bradykinin Receptor Antagonists; D002118:Calcium / Q000378:metabolism*; D002460:Cell Line; D003907:Dexamethasone / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D007424:Intracellular Fluid / Q000378:metabolism; D009355:Neomycin / Q000494:pharmacology; D010179:Pancreas / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D051381:Rats
D065168:Bradykinin Receptor Antagonists; C053748:B 4162; C051090:bradykinin, Leu(8)-des-Arg(9)-; D003907:Dexamethasone; D009355:Neomycin; D001920:Bradykinin; D002118:Calcium