Cloning of the rat steroid sulfatase gene (Sts), a non-pseudoautosomal X-linked gene that undergoes X inactivation.
Although the human steroid sulfatase (STS) gene has been cloned and characterized in detail, several attempts to clone its mouse homologue, with either anti-human STS antibodies or human STS cDNA probes, have failed, suggesting a substantial divergence between these genes. However, partial amino-terminal sequence from purified rat liver STS is very similar to its human counterpart, and sequence comparisons have revealed several domains that are conserved among all the sulfatases characterized to date. Thus, we used a degenerate-primer RT-PCR approach to amplify a 321-bp fragment from rat liver cDNA, which was used as a probe to clone and characterize the complete cDNA. Comparison of the protein coding region between the rat and human genes showed 66% homology both at the DNA and the protein levels. STS activity was conferred to STS(-) A9 cells upon transfection with a rat Sts expression construct, indicating the authenticity of the cloned cDNA. While Sts has been shown to be located in the mouse pseudoautosomal region, both physical and genetic mapping demonstrate that Sts is not pseudoautosomal in the rat. The overall genomic organization of rat Sts and human STS is very similar, except that the insertion site for intron 1 in the rat is 26 bp upstream from that in the human. Rat Sts is only 8.2 kb long, while the human STS spans over 146 kb.
Li
X M
XM
UCSF School of Medicine, Department of Pediatrics 94143, USA.
Salido
E C
EC
Gong
Y
Y
Kitada
K
K
Serikawa
T
T
Yen
P H
PH
Shapiro
L J
LJ
D000595:Amino Acid Sequence; D000818:Animals; D001192:Arylsulfatases / Q000235:genetics*; D001483:Base Sequence; D002874:Chromosome Mapping; D002875:Chromosomes; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004303:Dosage Compensation, Genetic; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D008040:Genetic Linkage; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D051381:Rats; D017207:Rats, Sprague-Dawley; D043266:Steryl-Sulfatase
D018076:DNA, Complementary; D001192:Arylsulfatases; D043266:Steryl-Sulfatase