A nitric oxide-sensitive electrode: requirement of lower oxygen concentration for detecting nitric oxide from the tissue.
In order to directly detect nitric oxide (NO) liberated from isolated tissue, a practical and convenient method using a nitric oxide-sensitive electrode is described. To avoid the nonselective signal caused by ionic substances, the electrode was covered with three layers but remains permeable for gaseous substances. In a solution bubbled with 20% oxygen (pO2, approximately 150 mm Hg), administration of S-nitroso-N-acetyl-d, l-penicillamine (SNAP) at concentrations greater than 10(-7) mol/L elicited an electrode response. Based on a comparison with the chemical determination of NO released from SNAP, the electrode may be able to detect nitric oxide around nmol/L. At least 30 nmol NO per liter in anoxic conditions was reported to be detected by this electrode (Matsui, 1995). In a specially designed small chamber, the electrode was attached on the surface of endothelial side of the isolated aorta of the guinea pig. When carbachol was added to the chamber, the electrode responded when the solution was bubbled with 20% but not with 40% or 95% of oxygen, suggesting a much faster decomposition of nitric oxide in the presence of higher concentrations of oxygen. The electrode response to carbachol was abolished in the presence of NG-monomethyl-L-arginine or nitro arginine. These results suggest that the electrode method described in this manuscript is suitable for detecting nitric oxide liberated from isolated tissues when comparatively low oxygen levels are present in the physiological salt solution.
Ishida
Y
Y
Mitsubishi Kasei Institute of Life Sciences, Tokyo, Japan.
Hashimoto
M
M
Fukushima
S
S
Masumura
S
S
Sasaki
T
T
Nakayama
K
K
Tamura
K
K
Murakami
E
E
Isokawa
S
S
Momose
K
K
D000818:Animals; D001013:Aorta, Thoracic / Q000378:metabolism; D001120:Arginine / Q000031:analogs & derivatives / Q000378:metabolism; D002217:Carbachol / Q000737:chemistry / Q000378:metabolism; D004566:Electrodes / Q000592:standards; D006168:Guinea Pigs; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000378:metabolism*; D009569:Nitric Oxide / Q000032:analysis / Q000378:metabolism*; D019335:Nitroarginine; D009638:Norepinephrine / Q000378:metabolism / Q000494:pharmacology; D010100:Oxygen / Q000378:metabolism*; D010313:Partial Pressure; D010396:Penicillamine / Q000031:analogs & derivatives / Q000378:metabolism / Q000494:pharmacology; D026423:S-Nitroso-N-Acetylpenicillamine; D014665:Vasodilator Agents / Q000378:metabolism / Q000494:pharmacology; D019323:omega-N-Methylarginine
D014665:Vasodilator Agents; D019335:Nitroarginine; D019323:omega-N-Methylarginine; D009569:Nitric Oxide; D026423:S-Nitroso-N-Acetylpenicillamine; D002217:Carbachol; D001120:Arginine; D010396:Penicillamine; D010100:Oxygen; D009638:Norepinephrine