Site-directed mutational analysis of the osmotically regulated proU promoter of Salmonella typhimurium.
We carried out PCR mutagenesis of the proU promoter of Salmonella typhimurium, in order to identify sequences important for its osmotic control. We obtained five mutations in the -35 element: two decreased the promoter strength, one increased it, and the others had no effect. However, none abolished osmotic control, suggesting that the sequence of the -35 element is not crucial for osmotic control.
Zhang
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X
Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907-1392, USA.
Fletcher
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SA
Csonka
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LN
D026905:Amino Acid Transport Systems; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D016297:Mutagenesis, Site-Directed; D011401:Promoter Regions, Genetic; D012486:Salmonella typhimurium / Q000235:genetics*
D026905:Amino Acid Transport Systems; D001426:Bacterial Proteins; D002352:Carrier Proteins; C060774:ProU protein, Bacteria