Wortmannin-mediated inhibition of phosphatidylinositol 3-kinase activity triggers apoptosis in normal and neoplastic B lymphocytes which are in cell cycle.
The B cell functional response following ligation of surface (s) IgM is dependent upon the differentiation stage of the population studied: cross-linking sIgM promotes proliferation of resting tonsillar follicular mantle (FM) B lymphocytes but induces apoptosis in the susceptible Epstein-Barr virus genome-negative Burkitt lymphoma (BL) cell line Ramos (Ramos-BL). This study investigates whether phosphatidylinositol-3-kinase (Pl3-kinase), which has been reported to be intimately involved in the regulation of cellular growth, plays a role in the regulation of these sigpromoted B cell responses, and uses the selective and irreversible inhibitor of Pl3-kinase activity, wortmannin (Wm). In Ramos-BL B cells, at 8 h post-treatment, Wm triggers a transient increase in apoptosis of 16 +/- 6.9% with a concomitant cellular loss of 16 +/- 6.1% from the G1 phase of cell cycle; [3H]thymidine incorporation also decreases by 33 +/- 5.0%, from 37,274 c.p.m. +/- 10% to 25,127 c.p.m. +/- 4.0%. Moreover, at 72 h culture, Wm inhibits anti-IgM-induced FM B lymphocyte levels of [3H]thymidine incorporation typically by 47% and triggers 80% apoptosis from the G0G1 phase of cell cycle. Ramos-BL B cells exhibit high basal levels of Pl3-kinase activity, as determined by immunoprecipitation with antibody to the p85 regulatory subunit of Pl3-kinase and 32P incorporation into phosphatidylinositol, which is not significantly affected by anti-IgM stimulation; by contrast, anti-IgM stimulates significant Pl3-kinase activity over negligible basal levels in FM B lymphocytes. Pre-treatment with Wm inhibits Pl3-kinase activity in both cell types. Taken together these data indicate that in Ramos-BL B cells sigM-triggered growth arrest and apoptosis is Pl3-kinase independent, whereas Pl3-kinase activity is critical for sIgM-triggered mitogenesis of FM B lymphocytes. Thus Pl3-kinase plays a pivotal role in the regulation of both normal and neoplastic B lymphocyte progression through the cell cycle, such that if this Pl3-kinase-dependent pathway is inhibited these cells default to apoptosis.
Padmore
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Department of Biochemistry, University of Oxford, UK.
Radda
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GK
Knox
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KA
D019870:1-Phosphatidylinositol 4-Kinase; D000730:Androstadienes / Q000378:metabolism*; D000888:Antibodies, Anti-Idiotypic / Q000502:physiology; D017209:Apoptosis; D001402:B-Lymphocytes / Q000201:enzymology* / Q000502:physiology*; D015153:Blotting, Western; D002051:Burkitt Lymphoma / Q000276:immunology*; D002453:Cell Cycle; D002455:Cell Division; D002478:Cells, Cultured; D004791:Enzyme Inhibitors / Q000378:metabolism*; D006801:Humans; D007075:Immunoglobulin M / Q000276:immunology / Q000378:metabolism; D008026:Ligation; D008854:Microscopy, Electron; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000037:antagonists & inhibitors*; D011947:Receptors, Antigen, B-Cell / Q000502:physiology; D014407:Tumor Cells, Cultured; D000077191:Wortmannin
D000730:Androstadienes; D000888:Antibodies, Anti-Idiotypic; D004791:Enzyme Inhibitors; D007075:Immunoglobulin M; D011947:Receptors, Antigen, B-Cell; D017853:Phosphotransferases (Alcohol Group Acceptor); D019870:1-Phosphatidylinositol 4-Kinase; D000077191:Wortmannin