A comparative map of the porcine and human genomes demonstrates ZOO-FISH and gene mapping-based chromosomal homologies.
ZOO-FISH with chromosome-specific DNA libraries (CSLs) from individual flow-sorted human chromosomes was applied on porcine metaphase chromosomes to establish segment homology between the pig and human karyotypes. Forty-seven porcine chromosomal segments corresponding to all human chromosomes except the Y were delineated, resulting in a nearly complete coverage of the porcine karyotype. The syntenic segments detected were further confirmed by the gene mapping information available in the two species. A map demarcating physical boundaries of human homologies on individual pig chromosomes is complemented with a detail survey of the physical and genetic linkage mapping data in the two species. The resultant map, thus, provides a comprehensive and updated comparative status of the human and porcine genomes.
Frönicke
L
L
Division of Human Biology and Human Genetics, University of Kaiserslautern, Erwin-Schroedingerstr., D-67663 Kaiserslautern, Germany.
Chowdhary
B P
BP
Scherthan
H
H
Gustavsson
I
I
D000818:Animals; D002874:Chromosome Mapping / Q000379:methods*; D005260:Female; D016678:Genome; D015894:Genome, Human; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D007621:Karyotyping; D008297:Male; D013045:Species Specificity; D013552:Swine / Q000235:genetics*