title: Platelet-mediated lymphocyte delivery to high endothelial venules.
abstract: Circulating lymphocytes gain access to lymph nodes owing to their ability to initiate rolling along specialized high endothelial venules (HEVs). One mechanism of rolling involves L-selectin binding to peripheral node addressin (PNAd) on HEVs. Activated platelets are shown to bind to circulating lymphocytes and to mediate rolling in HEVs, in vivo, through another molecule, P-selectin, which also interacts with PNAd. In vitro, activated platelets enhanced tethering of lymphocytes to PNAd and sustained lymphocyte rolling, even in the absence of functional L-selectin. Thus, a platelet pathway operating through P-selectin provides a second mechanism for lymphocyte delivery to HEVs.
authors.affiliation: Center for Blood Research, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA.
authors.forename: T G
authors.initials: TG
authors.lastname: Diacovo
authors.forename: K D
authors.initials: KD
authors.lastname: Puri
authors.forename: R A
authors.initials: RA
authors.lastname: Warnock
authors.forename: T A
authors.initials: TA
authors.lastname: Springer
authors.forename: U H
authors.initials: UH
authors.lastname: von Andrian
mesh_terms: D000818:Animals; D000954:Antigens, Surface / Q000378:metabolism*; D001792:Blood Platelets / Q000502:physiology*; D002448:Cell Adhesion; D002465:Cell Movement; D004730:Endothelium, Vascular / Q000166:cytology; D006801:Humans; D019041:L-Selectin / Q000502:physiology; D008024:Ligands; D008198:Lymph Nodes / Q000098:blood supply* / Q000166:cytology; D008214:Lymphocytes / Q000166:cytology / Q000502:physiology*; D008562:Membrane Glycoproteins / Q000378:metabolism; D008565:Membrane Proteins; D051379:Mice; D019007:P-Selectin / Q000378:metabolism; D015539:Platelet Activation; D016201:Receptors, Lymphocyte Homing / Q000378:metabolism; D014162:Transfection; D014407:Tumor Cells, Cultured; D014699:Venules / Q000166:cytology
chemical_list: D000954:Antigens, Surface; C069886:L-selectin counter-receptors; D008024:Ligands; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D019007:P-Selectin; C084882:P-selectin ligand protein; D016201:Receptors, Lymphocyte Homing; D019041:L-Selectin