Assembly of a 1-Mb restriction-mapped cosmid contig spanning the candidate region for Finnish congenital nephrosis (NPHS1) in 19q13.1.
We describe the assembly of a 1-Mb cosmid contig and restriction map spanning the candidate region for Finnish congenital nephrosis (NPHS1) in 19q13.1. The map was constructed from 16 smaller contigs assembled by fingerprinting, a BAC and a PAC clone, and 42 previously unmapped cosmids. In most cases, single-step cosmid walks were sufficient to join two previously assembled contigs, and all but one gap was filled from this cosmid contig library. The remaining gap of about 19 kb was spanned with a single BAC and a single PAC clone. EcoRI mapping of a dense set of overlapping clones validated the assembly of the map and indicated a length of 1040 kb for the contig. This high-resolution clone map provides an ideal resource for gene identification through cDNA selection, exon trapping, and DNA sequencing.
Olsen
A S
AS
Human Genome Center, Lawrence Livermore National Laboratory, Livermore, California, 94551, USA.
Georgescu
A
A
Johnson
S
S
Carrano
A V
AV
D002874:Chromosome Mapping; D002888:Chromosomes, Human, Pair 19; D003001:Cloning, Molecular; D003360:Cosmids; D015246:Deoxyribonuclease EcoRI; D005091:Exons; D005387:Finland / Q000453:epidemiology; D015723:Gene Library; D005808:Genes, Recessive; D005819:Genetic Markers; D006801:Humans; D015994:Incidence; D009401:Nephrosis / Q000151:congenital / Q000453:epidemiology / Q000235:genetics*; D015183:Restriction Mapping
D005819:Genetic Markers; D015246:Deoxyribonuclease EcoRI