title: Primary structure of alboaggregin-B purified from the venom of Trimeresurus albolabris.
abstract: The complete amino acid sequences of alpha and beta subunits of alboaggregin-beta are presented. The alpha and beta subunits were separated by reversed-phase HPLC after reduction and S-pyridylethylation, and their sequences were determined by analysis of peptides generated by enzymatic or chemical digestion. The alpha and beta subunits consist of 133 and 123 amino acid residues, respectively. The sequences are highly homologous to each other (41.4% identity) and also to those of the alpha and beta subunits of botrocetin (a von Willebrand factor modulator) and the A and B chains of factor IX/X binding protein from other snake venoms. It is also homologous to C-type lectins with a homodimeric structure, but it shows no lectin-like activity.
authors.affiliation: Department of Pharmaceutics, Gifu Pharmaceutical University, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Usami
authors.forename: M
authors.initials: M
authors.lastname: Suzuki
authors.forename: E
authors.initials: E
authors.lastname: Yoshida
authors.forename: Y
authors.initials: Y
authors.lastname: Sakurai
authors.forename: K
authors.initials: K
authors.lastname: Hirano
authors.forename: T
authors.initials: T
authors.lastname: Kawasaki
authors.forename: Y
authors.initials: Y
authors.lastname: Fujimura
authors.forename: K
authors.initials: K
authors.lastname: Titani
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D037263:Asialoglycoprotein Receptor; D002851:Chromatography, High Pressure Liquid; D003435:Crotalid Venoms / Q000737:chemistry* / Q000302:isolation & purification; D017839:Crotalus; D006801:Humans; D037102:Lectins / Q000737:chemistry; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000302:isolation & purification; D011956:Receptors, Cell Surface / Q000737:chemistry; D017386:Sequence Homology, Amino Acid
chemical_list: D037263:Asialoglycoprotein Receptor; D003435:Crotalid Venoms; D037102:Lectins; D046911:Macromolecular Substances; D010446:Peptide Fragments; D011956:Receptors, Cell Surface; C029748:Trimeresurus venoms; C030961:botrocetin