title: The application of microridge analysis in the diagnosis of gastro-oesophageal reflux disease.
abstract: BACKGROUND
  The percentage of epithelial surface area covered by microridges (%MR) seen during scanning electron microscopy of oesophageal biopsy specimens has previously been shown to correlate with symptomatic reflux disease, a result < or = 35% being abnormal. The aim of this study was to compare %MR with endoscopy, light microscopy, and pH monitoring results.
  
  
  METHODS
  Sixty-seven patients with heartburn were divided into oesophagitis or none on the basis of endoscopy and light microscopy findings and into those with and without abnormal acid reflux on the basis of pH monitoring.
  
  
  RESULTS
  The endoscopic and light microscopic oesophagitis groups had significantly greater degrees of acid reflux than those without oesophagitis (p < 0.05), even though neither the specific %MR nor the number of patients below the 35% cutoff showed any difference between those with and without endoscopic oesophagitis, light microscopic oesophagitis or those with normal and abnormal acid reflux on pH monitoring.
  
  
  CONCLUSIONS
  Despite the significant relationship between endoscopic and light microscopic oesophagitis and abnormal pH monitoring microridge analysis did not correlate with any of these variables
authors.affiliation: Dept. of Medicine, Queen's University, Belfast, UK.
authors.forename: B T
authors.initials: BT
authors.lastname: Johnston
authors.forename: S
authors.initials: S
authors.lastname: Nunn
authors.forename: J M
authors.initials: JM
authors.lastname: Sloan
authors.forename: J S
authors.initials: JS
authors.lastname: Collins
authors.forename: R J
authors.initials: RJ
authors.lastname: McFarland
authors.forename: S
authors.initials: S
authors.lastname: Parkin
authors.forename: K E
authors.initials: KE
authors.lastname: Carr
authors.forename: B J
authors.initials: BJ
authors.lastname: Collins
mesh_terms: D001706:Biopsy; D004942:Esophagitis, Peptic / Q000175:diagnosis* / Q000473:pathology; D004945:Esophagoscopy; D004947:Esophagus / Q000473:pathology*; D005260:Female; D005745:Gastric Acidity Determination; D005753:Gastric Mucosa / Q000473:pathology*; D005764:Gastroesophageal Reflux / Q000175:diagnosis* / Q000473:pathology; D006801:Humans; D008297:Male; D008855:Microscopy, Electron, Scanning; D008875:Middle Aged; D012680:Sensitivity and Specificity