Interaction of HIV Rev peptides with the Rev response element RNA.
As a model system for understanding RNA-protein interactions, we are studying the structure of peptides derived from HIV Rev in complex with an RNA derived from the Rev Response Element (RRE) using NMR spectroscopy. Formation of the Rev peptide-RRE complex is accompanied by formation of two purine-purine base pairs in the RRE. These base pairs create an unusual geometry that widens the major groove of the RRE RNA. The Rev peptide is predominantly in an alpha-helical conformation in the complex. The specific recognition of the RRE by Rev involves widening of the major groove to accommodate the Rev alpha-helix.
Williamson
J R
JR
Department of Chemistry, Massachusetts Institute of Technology, Cambridge 02139, USA.
Battiste
J L
JL
Mao
H
H
Frankel
A D
AD
D001483:Base Sequence; D001665:Binding Sites; D015694:Gene Products, rev / Q000737:chemistry / Q000378:metabolism*; D015752:Genes, env; D006678:HIV / Q000235:genetics* / Q000502:physiology*; D009682:Magnetic Resonance Spectroscopy; D008954:Models, Biological; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009690:Nucleic Acid Conformation; D011485:Protein Binding; D017433:Protein Structure, Secondary; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D014779:Virus Replication; D054321:rev Gene Products, Human Immunodeficiency Virus
D015694:Gene Products, rev; D012367:RNA, Viral; D054321:rev Gene Products, Human Immunodeficiency Virus