title: [Genetic differentiation of hantaviruses using the polymerase chain reaction and sequencing].
abstract: Thirty-two hantavirus strains and 8 samples of lung tissue from rodents collected in different regions of Russia have been examined by molecular biological methods. Two methodological approaches have been employed for the study of genetic relationships between the viruses: nested PCR assay and common RT-PCR with subsequent direct sequencing of 200 and 365 base pair of G2 protein encoding regions of M-segment, respectively, and the resultant sequences were compared with those of the prototype hantavirus. The study revealed a mosaic pattern of distribution of different hantavirus genotypes on the territory of Russia.
authors.forename: A E
authors.initials: AE
authors.lastname: Dekonenko
authors.forename: E A
authors.initials: EA
authors.lastname: Tkachenko
authors.forename: G Iu
authors.initials: GIu
authors.lastname: Lipskaia
authors.forename: T K
authors.initials: TK
authors.lastname: Dzagurova
authors.forename: A P
authors.initials: AP
authors.lastname: Ivanov
authors.forename: L I
authors.initials: LI
authors.lastname: Ivanov
authors.forename: R A
authors.initials: RA
authors.lastname: Slonova
authors.forename: S A
authors.initials: SA
authors.lastname: Markeshin
authors.forename: E A
authors.initials: EA
authors.lastname: Ivanidze
authors.forename: T M
authors.initials: TM
authors.lastname: Shutkova
authors.forename: V V
authors.initials: VV
authors.lastname: Iakimenko
authors.forename: V
authors.initials: V
authors.lastname: Chizhikov
mesh_terms: D000818:Animals; D003411:Arvicolinae; D001483:Base Sequence; D017931:DNA Primers; D005838:Genotype; D018063:Hantaan virus / Q000145:classification / Q000235:genetics*; D008969:Molecular Sequence Data; D009030:Mosaicism; D009115:Muridae; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D013045:Species Specificity
chemical_list: D017931:DNA Primers
vernacular_title: Geneticheskaia differentsiatsiia khantavirusov s pomoshch'iu polimeraznoĭ tsepnoĭ reaktsii i sekvenirovaniia.