Regulation of transforming growth factor-beta 1-mediated apoptosis in head and neck squamous cell carcinoma.
The apoptotic cell death in Cal-27 cells induced by exposure to transforming growth factor-beta 1 was inhibited by the endonuclease inhibitor aurintricarboxylic acid (ATA) in a concentration-dependent fashion. In vitro studies of cytotoxicity, DNA fragmentation, and protein synthesis by Cal-27 cell lines were performed. Inhibition of cytotoxicity as well as endonucleolytic DNA cleavage was detected. ATA did not inhibit cytotoxicity either via transforming growth factor cell-surface-receptor alteration or by inhibition of macromolecular synthesis. ATA-sensitive events occurred late during treatment. These data suggest that endonucleolytic DNA cleavage is a mandatory event leading to cell death in this system.
Duffey
D C
DC
Division of Head and Neck Surgery, University of California at Los Angeles School of Medicine, 90024-1624, USA.
Calcaterra
T C
TC
Lichtenstein
A K
AK
D017209:Apoptosis; D001312:Aurintricarboxylic Acid / Q000494:pharmacology; D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D004273:DNA, Neoplasm / Q000032:analysis; D004720:Endonucleases / Q000037:antagonists & inhibitors; D006258:Head and Neck Neoplasms / Q000473:pathology*; D006801:Humans; D014176:Protein Biosynthesis; D016212:Transforming Growth Factor beta / Q000502:physiology*; D014407:Tumor Cells, Cultured
D004273:DNA, Neoplasm; D016212:Transforming Growth Factor beta; D001312:Aurintricarboxylic Acid; D004720:Endonucleases