title: Stereospecific dihydroxylation of the styrene vinyl group by purified naphthalene dioxygenase from Pseudomonas sp. strain NCIB 9816-4.
abstract: Naphthalene dioxygenase (NDO) from Pseudomonas sp. strain NCIB 9816-4 adds both atoms of the dioxygen molecule to styrene to form (R)-l-phenyl-1,2-ethanediol. Product formation is tightly coupled to dioxygen consumption and NADH oxidation. NDO oxidizes styrene-d8 at almost the same initial rate as styrene. The results indicate that dioxygen activation by NDO is different from that by cytochrome P-450 and other monooxygenases, which oxidize styrene to styrene 1,2-oxide.
authors.affiliation: Department of Microbiology, University of Iowa, Iowa City, 52242, USA.
authors.forename: K
authors.initials: K
authors.lastname: Lee
authors.forename: D T
authors.initials: DT
authors.lastname: Gibson
mesh_terms: D049308:Dioxygenases; D006900:Hydroxylation; D009097:Multienzyme Complexes / Q000494:pharmacology*; D009243:NAD / Q000378:metabolism; D010084:Oxidation-Reduction; D010105:Oxygenases / Q000494:pharmacology*; D011549:Pseudomonas / Q000201:enzymology*; D020058:Styrene; D013343:Styrenes / Q000378:metabolism*
chemical_list: D009097:Multienzyme Complexes; D013343:Styrenes; D009243:NAD; D020058:Styrene; D010105:Oxygenases; D049308:Dioxygenases; C038703:naphthalene dioxygenase