[The nuclei of the follicular cells regulate steroidogenesis stimulated by a pituitary suspension in the follicles of the common frog in vitro].
It has already been shown that the follicle cell nuclei are not involved in regulation of amphibian oocyte maturation stimulated by the gonadotropic hormones (Skoblina and Kondrat'eva, 1992). In order to elucidate their involvement in regulation of steroidogenesis, we determined changes in the content of progesterone, testosterone, and estradiol-17beta in the common frog follicles stimulated by the pituitary suspension after preliminary treatment with actinomycin D and without it. Treatment with actinomycin D (5 micrograms/ml) reliably decreased the content of progesterone and increased that of testosterone. The content of estradiol-17beta in the control and actinomycin-treated follicles was below the method sensitivity. Treatment of the follicles with the inhibitor of chloride channels SITS (10 microM) reduced the progesterone content to the initial level, did not affect the testosterone content and suppressed oocyte maturation.
Skoblina
M N
MN
Huhtaniemi
I
I
Matikainen
T
T
D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid / Q000494:pharmacology; D000818:Animals; D002467:Cell Nucleus / Q000187:drug effects* / Q000378:metabolism; D003609:Dactinomycin / Q000494:pharmacology; D004958:Estradiol / Q000096:biosynthesis*; D005260:Female; D066298:In Vitro Techniques; D006080:Ovarian Follicle / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D010902:Pituitary Gland / Q000502:physiology*; D011374:Progesterone / Q000096:biosynthesis*; D011896:Rana temporaria; D013268:Stimulation, Chemical; D013535:Suspensions; D013739:Testosterone / Q000096:biosynthesis*; D013997:Time Factors; D014020:Tissue Extracts / Q000494:pharmacology*
D013535:Suspensions; D014020:Tissue Extracts; D003609:Dactinomycin; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid; D013739:Testosterone; D011374:Progesterone; D004958:Estradiol
Iadra follikuliarnykh kletok reguliruiut steroidogenez, stimulirovannyĭ suspenzieĭ gipofizov v follikulakh travianoĭ liagushki in vitro.