Daily short exposure of cultured mesothelial cells to lactated, high-glucose, low-pH peritoneal dialysis fluid induces a low-profile regenerative steady state.
This study was designed to evaluate cytotoxic effects and influence upon cell growth of cultured mesothelial cells exposed to modified 4.25% Dianeal dialysate fluid (M-199 in Dianeal solution, glucose 4.25 g, pH 5.2) (Expr. group), 60 min a day for a total period of follow-up of 13 consecutive days, compared with that observed in a control group (C). Beginning on day 7, the cell counts in group C were significantly higher than those observed at zero time (P < 0.05). Cell counts in the experimental group showed no significant differences between the first day of culture and each one of the 13 consecutive days of follow-up. Thymidine incorporation into DNA observed on the first day in C, was significantly higher (P < 0.01) beginning on the 10th day. Values observed in the experimental group were low during the whole period of follow-up. LDH mean values at each time interval, were significantly higher (P < 0.01 and < 0.001) for cells exposed to the dialysis solution. Repeated exposure of the mesothelium to 40 mMol/l lactate and high glucose concentrations induced severe cell injury and death, decreased cell growth and, consequently, a reduced rate of regeneration which is extended as long as the repeated exposure is maintained.
Shostak
A
A
Department of Nephrology, Central Emek Hospital, Afula, Israel.
Pivnik
K
K
Gotloib
L
L
D000818:Animals; D002452:Cell Count; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D015314:Dialysis Solutions / Q000494:pharmacology*; D004847:Epithelial Cells; D004848:Epithelium / Q000187:drug effects; D005947:Glucose / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D007770:L-Lactate Dehydrogenase / Q000187:drug effects / Q000378:metabolism; D007773:Lactates / Q000494:pharmacology*; D019344:Lactic Acid; D010529:Peritoneal Cavity / Q000166:cytology; D010530:Peritoneal Dialysis; D010537:Peritoneum / Q000166:cytology* / Q000187:drug effects; D051381:Rats; D013936:Thymidine / Q000378:metabolism
D015314:Dialysis Solutions; D007773:Lactates; D019344:Lactic Acid; D004247:DNA; D007770:L-Lactate Dehydrogenase; D005947:Glucose; D013936:Thymidine