title: Characterization of the NHA1 gene encoding a Na+/H+-antiporter of the yeast Saccharomyces cerevisiae.
abstract: The NHA1 gene (2958 nt) encoding a putative Na(+)/H(+) antiporter (986 aa) in Saccharomyces cerevisiae was cloned by selection based on increased NaCl tolerance. The putative protein is highly similar to sodium/proton antiporters from Schizosaccharomyces pombe (gene sod2), and Zygosaccharomyces rouxii (gene Z-SOD2). Overexpression of the NHA1 gene results in higher and partially pH-dependent tolerance to sodium and lithium; its disruption leads to an increased sensitivity towards these ions.
authors.affiliation: Laboratoire de Microbiologie et de Genetique, URA n-1481 Universite Louis Pasteur, Strasbourg, France.
authors.forename: C
authors.initials: C
authors.lastname: Prior
authors.forename: S
authors.initials: S
authors.lastname: Potier
authors.forename: J L
authors.initials: JL
authors.lastname: Souciet
authors.forename: H
authors.initials: H
authors.lastname: Sychrova
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D027682:Cation Transport Proteins; D003001:Cloning, Molecular; D004271:DNA, Fungal; D008565:Membrane Proteins / Q000378:metabolism*; D008969:Molecular Sequence Data; D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins; D017386:Sequence Homology, Amino Acid; D012965:Sodium Chloride / Q000494:pharmacology; D017923:Sodium-Hydrogen Exchangers / Q000235:genetics / Q000378:metabolism*
chemical_list: D027682:Cation Transport Proteins; D004271:DNA, Fungal; D008565:Membrane Proteins; C000631624:NHA1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D017923:Sodium-Hydrogen Exchangers; D012965:Sodium Chloride