title: A silent trans-splicing signal in the cuticlin-encoding gene of the plant-parasitic nematode Meloidogyne artiellia.
abstract: A gene (cut-1) coding for a cuticular protein, cuticlin-1, has been isolated and sequenced in the plant parasitic nematode, Meloidogyne artiellia. The nucleotide sequence revealed a typical eukaryotic-like organization, exons and introns bordered by canonical sequences. The 5' flanking region presents nematode-specific sequence motifis, including a trans-splicing signal. Studies on the expression of this gene demonstrated that, while in the adult females cut-1 is not expressed, the removal of the introns occurs in the eggs. These experiments also indicate that cis-splicing precedes the processing of the 5' untranslated region. In no case has a trans-spliced transcript been detected.
authors.affiliation: Dipartimento di Biochimica e Biologia Molecolare, Università di Bari, Italy. e103cd01@area.ba.cnr.it
authors.forename: C
authors.initials: C
authors.lastname: De Giorgi
authors.forename: F
authors.initials: F
authors.lastname: De Luca
authors.forename: F
authors.initials: F
authors.lastname: Lamberti
mesh_terms: D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018509:DNA, Helminth; D018628:Gene Dosage; D015801:Helminth Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D010944:Plants / Q000469:parasitology; D012326:RNA Splicing; D018510:RNA, Helminth / Q000378:metabolism*; D015398:Signal Transduction; D014158:Transcription, Genetic; D014431:Tylenchoidea / Q000235:genetics* / Q000254:growth & development
chemical_list: D018509:DNA, Helminth; D015801:Helminth Proteins; D018510:RNA, Helminth