DNA sequencing by delayed extraction-matrix-assisted laser desorption/ionization time of flight mass spectrometry.
Matrix-assisted laser desorption/ionization (MALDI) time of flight mass spectrometry was used to detect and order DNA fragments generated by Sanger dideoxy cycle sequencing. This was accomplished by improving the sensitivity and resolution of the MALDI method using a delayed ion extraction technique (DE-MALDI). The cycle sequencing chemistry was optimized to produce as much as 100 fmol of each specific dideoxy terminated fragment, generated from extension of a 13-base primer annealed on 40- and 50-base templates. Analysis of the resultant sequencing mixture by DE-MALDI identified the appropriate termination products. The technique provides a new non-gel-based method to sequence DNA which may ultimately have considerable speed advantages over traditional methodologies.
Roskey
M T
MT
PerSeptive Biosystems, Framingham, MA 01701, USA.
Juhasz
P
P
Smirnov
I P
IP
Takach
E J
EJ
Martin
S A
SA
Haff
L A
LA
D001483:Base Sequence; D004247:DNA / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D005069:Evaluation Studies as Topic; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000235:genetics; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA / Q000379:methods* / Q000706:statistics & numerical data; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods* / Q000706:statistics & numerical data
D017931:DNA Primers; D009838:Oligodeoxyribonucleotides; D004247:DNA