title: A classical strain of Vibrio cholerae with diminished ability to process the proteolytically sensitive site in the A subunit of cholera toxin.
abstract: Vibrio cholerae O1, No. 31, a strain isolated from a patient with mild diarrhea, produced mainly the unnicked cholera toxin. The amount of toxin that had accumulated in the cells was approximately 200 times lower than that secreted into the culture medium. When the unnicked toxin was purified by three successive column chromatographies and then extracted from the polyacrylamide gel, the unnicked toxin showed two bands corresponding to the A and B subunits by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and the A1 fragment was detected by trypsinization. Biological and enzymatic activities of the purified toxin with trypsinization were identical to those of cholera toxin from V. cholerae 569B as seen in the rabbit skin permeability test and the NAD:agmatine ADP-ribosyltransferase assay. DNA sequences of the A and B subunits were identical to those of the A- and B-subunit genes from the El Tor 2125 and classical 0395 strains, respectively. These data suggest that the wild V. cholerae strain, No. 31, produces a toxin identical to toxins previously reported in the literature and secretes it without accumulation in the cell, as is the case with other strains. However, strain No. 31's ability to nick the toxin is diminished compared with such abilities of other strains.
authors.affiliation: Department of Bacteriology, Institute of Tropical Medicine, Nagasaki University, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Ichinose
authors.forename: T
authors.initials: T
authors.lastname: Tsuji
authors.forename: M
authors.initials: M
authors.lastname: Kato
authors.forename: B C
authors.initials: BC
authors.lastname: Neves
authors.forename: K
authors.initials: K
authors.lastname: Morita
authors.forename: M
authors.initials: M
authors.lastname: Ehara
authors.forename: T
authors.initials: T
authors.lastname: Hirayama
mesh_terms: D000818:Animals; D002772:Cholera Toxin / Q000378:metabolism*; D010450:Endopeptidases / Q000502:physiology*; D006801:Humans; D011817:Rabbits; D014734:Vibrio cholerae / Q000378:metabolism*
chemical_list: D002772:Cholera Toxin; D010450:Endopeptidases