title: Depurination of A4256 in 28 S rRNA by the ribosome-inactivating proteins from barley and ricin results in different ribosome conformations.
abstract: Ribosomal function in protein synthesis requires dynamic flexibility of the ribosomal structure. The two translational inhibitors derived from seeds of ricin and barley destroy the dynamic properties of the ribosome by selective depurination of A4256 in the phylogenetically conserved alpha-sarcin/ricin loop of mouse 28 S rRNA. As the alpha-sarcin/ricin loop is involved in binding of elongation factors to the ribosome, depurination blocks the protein synthesis elongation cycle. Depurination by the barley translational inhibitor (BTI) mainly effects eEF-1 alpha related functions, while ricin interferes with the interaction of eEF-2 with the ribosome. Analysis of the ribosomal structure after inhibitor shows that the accessibility of the rRNAs for single-strand-specific chemical modification was altered. Reactivity changes were seen in domains I, II and V of 28 S rRNA and in 5 S rRNA. A majority of the reactivity changes were found in putative functional regions of the rRNAs, such as the regions involved in peptidyltransferase activity, subunit interaction and in the binding of elongation factors. Most of the observed structural changes made the rRNAs less accessible for chemical modification, suggesting that the ribosomal particles became less flexible after inhibitor treatment. Moreover, the modification patterns obtained from the two inhibitor-treated ribosomal particles were only partly overlapping, indicating that the structure of the large ribosomal subunit differed after ricin and BTI treatment. Surprisingly, depurination in the alpha-sarcin/ricin loop of 28 S rRNA also affected the structure of the 3' major domain in 18 S rRNA.
authors.affiliation: Department of Zoological Cell Biology, Arrhenius Laboratories E5, Stockholm University, Sweden.
authors.forename: L
authors.initials: L
authors.lastname: Holmberg
authors.forename: O
authors.initials: O
authors.lastname: Nygård
mesh_terms: D000225:Adenine / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D001467:Hordeum / Q000737:chemistry*; D008274:Magnesium / Q000378:metabolism / Q000494:pharmacology; D051379:Mice; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D037121:Plant Lectins; D010940:Plant Proteins / Q000494:pharmacology*; D011687:Purines / Q000737:chemistry / Q000378:metabolism; D012337:RNA, Ribosomal, 18S / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D012339:RNA, Ribosomal, 28S / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism; D012341:RNA, Ribosomal, 5S / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D012270:Ribosomes / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D012276:Ricin / Q000737:chemistry*
chemical_list: D017931:DNA Primers; D037121:Plant Lectins; D010940:Plant Proteins; D011687:Purines; D012337:RNA, Ribosomal, 18S; D012339:RNA, Ribosomal, 28S; D012341:RNA, Ribosomal, 5S; D012276:Ricin; D008274:Magnesium; D000225:Adenine; C030985:purine