A role for BP-3/BST-1 antigen in early T cell development.
In the mouse thymus, pre-T cells are defined by their CD3(-)CD4(-)CD8(-) triple-negative, CD44lo/- CD25+ phenotype. We made a rat mAb IF-7, that, among all T cell subsets analyzed, reacted exclusively with pre-T cells. Molecular cloning revealed that the antigen recognized by IF-7 was identical to BP-3/BST-1, a glycosyl-phosphatidylinositol-linked, CD38-related molecule previously described as a possible co-activation molecule of pre-B cells. We found that IF-7 cross-linking enhances the proliferative response of sorted pre-T cells to anti-CD3 stimulation. In addition, IF-7 enhances and accelerates the development of fetal thymic organ culture (FTOC), although the gamma delta lineage is unaffected by the treatment. In addition, sorted IF-7+ pre-T cells give preferentially rise to alpha beta TCR+ thymocytes in FTOC. Our observations strongly suggest that BP-3/BST-1 is implicated in both early B and T cell growth and development, and is an early marker for the alpha beta lineage.
Vicari
A P
AP
DNAX Research Institute, Palo Alto, CA 94304-1104, USA.
Bean
A G
AG
Zlotnik
A
A
D036541:ADP-ribosyl Cyclase; D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry; D015703:Antigens, CD; D017252:CD3 Complex / Q000276:immunology; D005260:Female; D058851:GPI-Linked Proteins; D006410:Hematopoiesis / Q000276:immunology*; D007519:Isoantigens / Q000276:immunology*; D008213:Lymphocyte Activation; D008297:Male; D008562:Membrane Glycoproteins / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D018345:Mice, Knockout; D016513:Mice, SCID; D009924:Organ Culture Techniques; D011247:Pregnancy; D013234:Stem Cells / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D013950:Thymus Gland / Q000166:cytology / Q000196:embryology
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D017252:CD3 Complex; D058851:GPI-Linked Proteins; D007519:Isoantigens; D008562:Membrane Glycoproteins; D036541:ADP-ribosyl Cyclase; C087521:ADP-ribosyl cyclase 2