Regional assignment of human ESTs by whole-genome radiation hybrid mapping.
The UK HGMP Resource Centre's collection of human partial cDNA sequences (ESTs) have been examined for suitability for mapping by PCR on a panel of somatic cell hybrids. The chromosomal assignments of 92 ESTs were determined with a monochromosomal hybrid panel, and a subset of 45 were linked to genetic markers with a panel of whole-genome radiation hybrids (WG-RHs). These results demonstrate the potential of WG-RHs to construct a transcript map of the human genome.
Hayes
P D
PD
HGMP Resource Centre, Hinxton, Cambridgeshire, UK.
Schmitt
K
K
Jones
H B
HB
Gyapay
G
G
Weissenbach
J
J
Goodfellow
P N
PN
D001483:Base Sequence; D002874:Chromosome Mapping; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics; D016678:Genome; D006801:Humans; D006822:Hybrid Cells / Q000528:radiation effects; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D016324:Sequence Tagged Sites
D017931:DNA Primers; D018076:DNA, Complementary