Genetic analysis of plasmid determinants for microcin J25 production and immunity.
Microcin J25 (MccJ25) is a small peptide antibiotic produced by an Escherichia coli strain isolated from human feces. The genetic determinants for MccJ25 synthesis and immunity have been cloned from the low-copy-number wild-type plasmid pTUC1OO into the compatible vectors pBR322 and pACYC184. Physical and phenotypical analysis of insertion mutations and complementation tests defined three contiguous genes involved in MccJ25 production which span a region of about 2.2 kb. Immunity to the antibiotic is provided by an additional gene adjacent to the production region.
Solbiati
J O
JO
Departamento de Bioquímica de la Nutrición, Instituto Superior de Investigaciones Biológicas, Tucumán, Argentina.
Ciaccio
M
M
Farías
R N
RN
Salomón
R A
RA
D001430:Bacteriocins / Q000235:genetics*; D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D016254:Mutagenesis, Insertional; D010957:Plasmids; D015183:Restriction Mapping
D001430:Bacteriocins; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; C020411:microcin