[Hydroxyethyl starch for reversing edema in short-term culture media for donor corneas].
OBJECTIVE
To investigate the feasibility to use hydroxyethylstarch as an alternative deswelling additive in short-term preservation media.


METHODS
Corneoscleral discs were prepared from pairs of eye balls of freshly slaughtered pigs. Corneas were stored in MEM-medium containing either 10% or 20% hydroxyethylstarch 450 000 at 4 degrees C in a refrigerator. Subsequently, the tissue was stored for 24 hours in organ culture at 37 degrees C in MEM-medium containing 10% fetal calf serum to detect latent endothelial cell damage. Mate corneas were treated the same except for being stored in Optisol GS during 4 degrees C storage. We determined corneal endothelial cell density, stromal thickness, and glucose concentration in the medium directly after preparation, after short-term storage at 4 degrees C, and after subsequent organ culture at 37 degrees C. Scanning electron microscopy of corneal endothelium was performed at each step during the experimental course.


RESULTS
We did not observe any significant differences in endothelial-cell density between experimental groups and control groups. No decrease in endothelial-cell density was observed during the course of experiments. No increase in stromal thickness was determined in any group after short-term storage at 4 degrees C. Corneas stored in medium containing 20% hydroxyethylstarch showed a decrease in stromal thickness after short-term storage. After subsequent organ culture all corneas displayed a uniform stromal swelling. Glucose concentrations in the media decreased in all groups during the experiment. In scanning-electron microscopy we observed a reversible degeneration of cell borders after storage at 4 degrees C. Additionally, corneas stored in Optisol GS showed a reversible cobblestone appearance at this stage of the experiments.


CONCLUSIONS
Hydroxyethylstarch appears to be an alternative to the use of dextran and chondroitin sulfate as a deswelling additive in corneal preservation media.
Hagenah
M
M
Augenklinik Medizinische Hochschule Hannover.
Halberstadt
M
M
Wehming
S
S
Brewitt
H
H
Winter
R
R
D000818:Animals; D002452:Cell Count; D003319:Corneal Stroma / Q000187:drug effects / Q000473:pathology; D016039:Corneal Transplantation / Q000473:pathology*; D015925:Cryopreservation; D003470:Culture Media; D004728:Endothelium, Corneal / Q000187:drug effects* / Q000473:pathology; D006895:Hydroxyethyl Starch Derivatives / Q000494:pharmacology*; D008855:Microscopy, Electron, Scanning; D009924:Organ Culture Techniques; D013552:Swine
D003470:Culture Media; D006895:Hydroxyethyl Starch Derivatives
Hydroxyäthylstärke als Entquellungssubstanz in Kurzzeitkulturmedien für Spenderhornhäute.