title: Study of second messenger levels and of sugar catabolism enzyme activities in transformed cells resistant to ionizing radiations.
abstract: We measured the level of second messengers, the activity of carbohydrate metabolism enzymes, and the resistance to ionizing radiations in normal 32D hematopoietic cells, in v-erbB transformants and in spontaneous transformants. v-erbB and spontaneous transformants were resistant to radiations as compared with their normal counterpart. The second messenger diacylglycerol was elevated in radioresistant clones. Only v-erbB transformants showed increase of the activities of enolase and glucose-6-phosphate dehydrogenase. v-erbB-transformed NIH/3T3 cells, selected as control, showed identical correlation between radioresistance, increase of diacylglycerol, and of enolase and glucose-6-phosphate dehydrogenase activity. These results indicate that increase of diacylglycerol is correlated with resistance to the killing effect of ionizing radiations and could be proposed as a marker of radioresponse.
authors.affiliation: Institute of General Pathology, University of Firenze, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Pacini
authors.forename: M
authors.initials: M
authors.lastname: Ruggiero
authors.forename: F
authors.initials: F
authors.lastname: Casamassima
authors.forename: M A
authors.initials: MA
authors.lastname: Santucci
authors.forename: F
authors.initials: F
authors.lastname: Milano
authors.forename: F
authors.initials: F
authors.lastname: Ranaldi
authors.forename: S
authors.initials: S
authors.lastname: Vanni
authors.forename: E
authors.initials: E
authors.lastname: Giachetti
mesh_terms: D016475:3T3 Cells / Q000378:metabolism / Q000528:radiation effects; D000818:Animals; D050260:Carbohydrate Metabolism; D002460:Cell Line; D002461:Cell Line, Transformed; D002471:Cell Transformation, Neoplastic / Q000528:radiation effects*; D004075:Diglycerides / Q000378:metabolism; D004307:Dose-Response Relationship, Radiation; D018773:Genes, erbB-1 / Q000235:genetics; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism*; D006412:Hematopoietic Stem Cells / Q000201:enzymology / Q000378:metabolism* / Q000528:radiation effects*; D007295:Inositol Phosphates / Q000378:metabolism; D007377:Interleukin-3 / Q000378:metabolism / Q000494:pharmacology; D051379:Mice; D010751:Phosphopyruvate Hydratase / Q000378:metabolism*; D010767:Phosphorylcholine / Q000378:metabolism; D011836:Radiation Tolerance; D014162:Transfection
chemical_list: D004075:Diglycerides; D007295:Inositol Phosphates; D007377:Interleukin-3; D010767:Phosphorylcholine; D005954:Glucosephosphate Dehydrogenase; D010751:Phosphopyruvate Hydratase