First report of the inhibition of arbuscular mycorrhizal infection of Pisum sativum by specific and irreversible inhibition of polyamine biosynthesis or by gibberellic acid treatment.
DFMO (alpha-DL-difluoromethylornithine), a specific irreversible inhibitor of ornithine decarboxylase (ODC), a polyamine biosynthetic pathway enzyme, strongly inhibits root growth and arbuscular mycorrhizal infection of Pisum sativum (P56 myc+, isogenic mutant of cv. Frisson). This inhibition is reversed when exogenous polyamine (putrescine) is included in the DFMO treatment, showing that the effect of DFMO on arbuscular mycorrhizal infection is indeed due to putrescine limitation and suggesting that ODC may have a role in root growth and mycorrhizal infection. However, treatment with gibberellic acid (GA3) which increased root titers of polyamines strongly inhibited arbuscular mycorrhizal development. The possible role of polyamines in the regulation of the development of arbuscular mycorrhizal infection is discussed.
El Ghachtouli
N
N
Laboratoire de Phytoparasitologie INRA-CNRS, SGAP, INRA, BV 1540, Dijon, France.
Martin-Tanguy
J
J
Paynot
M
M
Gianinazzi
S
S
D002103:Cadaverine / Q000378:metabolism; D000518:Eflornithine / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005658:Fungi / Q000502:physiology*; D005875:Gibberellins / Q000494:pharmacology*; D009154:Mutation; D065108:Ornithine Decarboxylase Inhibitors; D018532:Pisum sativum / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism / Q000382:microbiology*; D010935:Plant Diseases; D010937:Plant Growth Regulators / Q000494:pharmacology; D018517:Plant Roots / Q000254:growth & development / Q000378:metabolism; D011073:Polyamines / Q000378:metabolism* / Q000494:pharmacology; D011700:Putrescine / Q000378:metabolism / Q000494:pharmacology; D013095:Spermidine / Q000378:metabolism; D013096:Spermine / Q000378:metabolism
D004791:Enzyme Inhibitors; D005875:Gibberellins; D065108:Ornithine Decarboxylase Inhibitors; D010937:Plant Growth Regulators; D011073:Polyamines; D013096:Spermine; C007842:gibberellic acid; D002103:Cadaverine; D013095:Spermidine; D011700:Putrescine; D000518:Eflornithine