[Ribosomal RNA degradation in gram-negative and gram-positive bacteria under the action of minimal bactericidal doses of chlorhexidine and heating].
The mechanism of the minimal bactericidal action of chlorhexidine, an antiseptic, and heating was studied. The bactericidal doses (BD99) of the above factors were determined with respect to the representatives of 5 families: Enterobacteriaceae, Pseudomonas, Vibrio, Bacillus and Bifidobacterium. Their effect on the ribosomes in the cells of the exponentially growing cultures was estimated. It was shown that these factors induced selective damage of the 30S subunits in the gram-negative bacteria at the account of a specific degradation rRNA in them while in the gram-positive bacteria there was observed no rRNA degradation under such conditions. Differences in the mechanisms of the ribosome damage in the cells of the gram-negative bacteria of the various families were detected. Since the above factors at the levels used had no effect on the bacterial ribosomes in vitro, it was suggested that the primary link in the damages was the cell membrane while the ribosome degradation was secondary, though biologically the process leading to the bacteria death was of great importance.
Lishchenko
N N
NN
Volkodav
L V
LV
D000891:Anti-Infective Agents, Local / Q000494:pharmacology*; D002710:Chlorhexidine / Q000494:pharmacology*; D006090:Gram-Negative Bacteria / Q000187:drug effects* / Q000235:genetics; D006094:Gram-Positive Bacteria / Q000187:drug effects* / Q000235:genetics; D006358:Hot Temperature; D012329:RNA, Bacterial / Q000378:metabolism*; D012335:RNA, Ribosomal / Q000378:metabolism*
D000891:Anti-Infective Agents, Local; D012329:RNA, Bacterial; D012335:RNA, Ribosomal; D002710:Chlorhexidine
Osobennosti degradatsii ribosomal'nykh RNK u gramotritsatel'nykh i grampolozhitel'nykh bakteriĭ pod deĭstviem minimal'nykh backteritsidnykh doz khlorgeksidina i nagrevaniia.