Interferon response depends on viral transcription in human papillomavirus-containing lesions.
Human papillomaviruses (HPVs) express various proteins which have been proven to interact with some cellular functions playing a role in cell cycle progression and differentiation. Therefore, these viral gene products might be candidates for interfering with IFN-mediated antiproliferative actions. Condyloma biopsies from patients subsequently demonstrated to be responsive or non-responsive to IFN treatment were investigated. mRNA levels of HPV genes and IFN-responsive genes were determined by RT-PCR and correlated with IFN responsiveness. Patients clinically nonresponsive to IFN demonstrated a characteristic HPV transcriptional activity differing from responder patients. Nonresponders expressed mostly early E7 mRNAs; responders demonstrated higher expression of the late L1 gene. This differential transcription of infecting HPV also correlated with the extent of IFN mediated antiproliferative effect. A hypothesis for further study is that HPV E7 may inhibit IFN responsiveness, while HPV L1 may promote IFN responsiveness.
Arany
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Department of Microbiology, University of Texas Medical Branch, Galveston 77555-1019, USA.
Goel
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Tyring
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D000998:Antiviral Agents / Q000494:pharmacology / Q000627:therapeutic use*; D003218:Condylomata Acuminata / Q000628:therapy* / Q000821:virology; D004351:Drug Resistance; D015967:Gene Expression Regulation, Viral / Q000187:drug effects*; D005814:Genes, Viral; D006801:Humans; D000077190:Interferon alpha-2; D016898:Interferon-alpha / Q000494:pharmacology / Q000627:therapeutic use*; D009856:Oncogene Proteins, Viral / Q000096:biosynthesis / Q000235:genetics*; D027383:Papillomaviridae / Q000187:drug effects / Q000235:genetics* / Q000502:physiology; D030361:Papillomavirus Infections / Q000628:therapy* / Q000821:virology; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis; D012367:RNA, Viral / Q000096:biosynthesis; D011994:Recombinant Proteins; D014158:Transcription, Genetic; D014412:Tumor Virus Infections / Q000628:therapy* / Q000821:virology
D000998:Antiviral Agents; D000077190:Interferon alpha-2; D016898:Interferon-alpha; D009856:Oncogene Proteins, Viral; D012333:RNA, Messenger; D012367:RNA, Viral; D011994:Recombinant Proteins