Effects of traditional herbal medicine on gastric mucin against ethanol-induced gastric injury in rats.
The effect of the traditional herbal medicine, Rikkunshi-to and its component crude drugs, Zingiberis Rhizoma and Glycyrrhizae Radix, on the gastric mucin was studied using a method developed to separate and quantify the mucin localized in the different layers of rat gastric mucosa. The oral administration of spray-dried extract to Rikkunshi-to (1000 mg/kg), Zingiberis Rhizoma (500 mg/kg) and Glycyrrhizae Radix (500 mg/kg) significantly prevented gastric mucosal damage induced by 70% ethanol in rats. In ethanol-treated rats the mucin content of the deep mucosa was reduced, and the reduction of the deep corpus mucin content was significantly inhibited by pretreatment of Rikkunshi-to and Zingiberis Rhizoma. Rikkunshi-to and Glycyrrhizae Radix pretreatment increased the surface mucin content by 140 and 146%, respectively. The effect on the gastric mucin by each drug differed in the different layers of the gastric mucosa.
Goso
Y
Y
Department of Biochemistry, Kitasato University School of Medicine, Sagamihara, Japan.
Ogata
Y
Y
Ishihara
K
K
Hotta
K
K
D000284:Administration, Oral; D000704:Analysis of Variance; D000818:Animals; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D000431:Ethanol / Q000008:administration & dosage / Q000633:toxicity*; D005753:Gastric Mucosa / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D006471:Gastrointestinal Hemorrhage / Q000517:prevention & control; D008297:Male; D009077:Mucins / Q000187:drug effects / Q000378:metabolism*; D017830:Octoxynol / Q000737:chemistry; D008517:Phytotherapy; D051381:Rats; D017208:Rats, Wistar
D004365:Drugs, Chinese Herbal; D009077:Mucins; C084136:liu-jun-zi-tang; D000431:Ethanol; D017830:Octoxynol