The half-life of RAG-1 protein in precursor B cells is increased in the absence of RAG-2 expression.
Site-specific recombination of immunoglobulin and T cell receptor gene segments in B and T lymphocytes is dependent on the expression of two recombinant activation genes, Rag-1 and Rag-2. Here, we show that RAG-1 protein turnover in pre-B cells depends on the expression of RAG-2. The apparent half-life of RAG-1 protein is increased when RAG-2 is not expressed in differentiating pre-B cells.
Grawunder
U
U
Basel Institute for Immunology, Switzerland.
Schatz
D G
DG
Leu
T M
TM
Rolink
A
A
Melchers
F
F
D000818:Animals; D001402:B-Lymphocytes / Q000378:metabolism*; D004268:DNA-Binding Proteins; D005260:Female; D005786:Gene Expression Regulation; D006207:Half-Life; D006412:Hematopoietic Stem Cells / Q000378:metabolism*; D018398:Homeodomain Proteins; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008817:Mice, Mutant Strains; D008822:Mice, Transgenic; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis; D011995:Recombination, Genetic
D004268:DNA-Binding Proteins; D018398:Homeodomain Proteins; D011506:Proteins; D012333:RNA, Messenger; C499308:Rag2 protein, mouse; C064655:V(D)J recombination activating protein 2; C064658:RAG-1 protein