title: Depiction of infarct frequency distribution by computer-assisted image mapping in rat brains with middle cerebral artery occlusion. Comparison of photothrombotic and intraluminal suture models.
abstract: OBJECTIVE
  Histopathologic analysis of experimental brain damage has traditionally been performed by measuring areas of infarction and/or selective neuronal alterations on a section-by-section basis in individual animals. For series containing multiple replicate animals, quantitation of tissue injury is typically performed at similar coronal levels throughout an experimental group. A means of facilitating pictorial group comparisons of these histopathologic alterations between different series of replicate studies is highly desirable.
  
  
  METHODS
  We introduce a newly designed approach to achieve this goal, based on a linear affine transformation that is used to map corresponding sections at the same anatomic level into a common template to yield a frequency distribution map depicting the aggregate data set. We have applied this approach to compare the histopathologic features of two models of middle cerebral artery (MCA) occlusion in rats: (1) photothrombotically induced permanent distal MCA occlusion in spontaneously hypertensive rats (SHR) and (2) temporary MCA occlusion by intraluminal suture in Wistar rats.
  
  
  RESULTS
  The brains of SHR rats with permanent distal MCA occlusion showed a high frequency of infarction involving the dorsolateral and lateral portions of the ipsilateral neocortex, whereas Wistar rats with 90-minute MCA suture occlusion showed a zone of infarction largely concentrated in the dorsolateral portion of the ipsilateral caudoputamen. Infarct frequency distributions for the two animal groups were compared statistically at three corresponding anatomic levels by Fisher's exact test; the resulting statistical parametric maps are shown.
  
  
  CONCLUSIONS
  With the use of frequency distribution maps, the pattern of trends within a group can be observed coronally or three-dimensionally. One can directly access data as to numbers of rats with infarction for any point on the map. Studies performed under different experimental conditions can also be compared with one another by means of the generated data sets.
authors.affiliation: Department of Neurology, University of Miami (Fla) School of Medicine 33101, USA.
authors.forename: W
authors.initials: W
authors.lastname: Zhao
authors.forename: M D
authors.initials: MD
authors.lastname: Ginsberg
authors.forename: R
authors.initials: R
authors.lastname: Prado
authors.forename: L
authors.initials: L
authors.lastname: Belayev
mesh_terms: D000818:Animals; D001157:Arterial Occlusive Diseases / Q000209:etiology / Q000473:pathology*; D002545:Brain Ischemia / Q000209:etiology / Q000473:pathology; D001931:Brain Mapping / Q000379:methods*; D002421:Caudate Nucleus / Q000473:pathology; D002539:Cerebral Arterial Diseases / Q000209:etiology / Q000473:pathology*; D002540:Cerebral Cortex / Q000473:pathology; D002544:Cerebral Infarction / Q000209:etiology / Q000473:pathology*; D004195:Disease Models, Animal; D005456:Fluorescent Dyes; D007091:Image Processing, Computer-Assisted; D002542:Intracranial Embolism and Thrombosis / Q000209:etiology / Q000473:pathology; D002546:Ischemic Attack, Transient / Q000209:etiology / Q000473:pathology; D007834:Lasers; D008297:Male; D017319:Photosensitizing Agents; D011699:Putamen / Q000473:pathology; D051381:Rats; D011918:Rats, Inbred SHR; D017208:Rats, Wistar; D012395:Rose Bengal; D013537:Sutures
chemical_list: D005456:Fluorescent Dyes; D017319:Photosensitizing Agents; D012395:Rose Bengal