title: Preservation of myocardial blood flow by calcium antagonists does not prevent attenuation of regional myocardial function after repetitive brief periods of myocardial ischaemia in the rat heart.
abstract: The aim of this study was to assess the effect of two different calcium channel blockers on myocardial blood flow and function in a rat model of myocardial 'stunning' by repeated short episodes of ischaemia ('repetitive ischaemia'). In an open chest rat model, the left anterior descending coronary artery was ligated for 10 min followed by 15 min reperfusion. In total, five periods of ischaemia and reperfusion were performed. Myocardial blood flow was assessed by the hydrogen clearance technique and systolic thickening fraction by pulsed Doppler. After five episodes of ischaemia, myocardial blood flow adn myocardial thickening in the ischaemic area were reduced by 60 +/- 8% and 52 +/- 7% (n=9), respectively, as compared to baseline. Continuous intravenous infusion of the calcium channel blockers nifedipine (n=6) and gallopamil (n=6), started 20 min prior to onset of ischaemia, attenuated the ischaemia-induced decrease of myocardial perfusion. Nifedipine was the most effective with only 5 +/- 2% reduction in blood flow after five ischaemic episodes, whereas reduction of myocardial blood flow was 30 +/- 4% in the presence of gallopamil. However, neither nifedipine nor gallopamil were able to prevent regional ventricular dysfunction induced by repetitive ischaemia. Despite the preservation of myocardial blood flow following repetitive ischaemia, calcium channel blockers do not prevent ischaemia-induced reduction of myocardial function in the ischaemic area.
authors.affiliation: Medizinische Universitätsklinik III, Ruprecht-Karls-Universität, Heidelberg, Germany.
authors.forename: C P
authors.initials: CP
authors.lastname: Tiefenbacher
authors.forename: R
authors.initials: R
authors.lastname: Zimmermann
authors.forename: N
authors.initials: N
authors.lastname: Parekh
authors.forename: K
authors.initials: K
authors.lastname: Amann
authors.forename: H
authors.initials: H
authors.lastname: Tillmanns
authors.forename: B
authors.initials: B
authors.lastname: Rauch
authors.forename: W
authors.initials: W
authors.lastname: Kübler
mesh_terms: D000818:Animals; D001783:Blood Flow Velocity / Q000187:drug effects; D002121:Calcium Channel Blockers / Q000627:therapeutic use*; D005260:Female; D005711:Gallopamil / Q000627:therapeutic use*; D007262:Infusions, Intravenous; D017202:Myocardial Ischemia / Q000473:pathology / Q000503:physiopathology* / Q000517:prevention & control; D009543:Nifedipine / Q000627:therapeutic use*; D051381:Rats; D011920:Rats, Inbred WF
chemical_list: D002121:Calcium Channel Blockers; D005711:Gallopamil; D009543:Nifedipine