Chromosomal localization and expression pattern of the RNase L inhibitor gene.
2-5A-Dependent RNase (RNase L), an important component of the 2-5A pathway, is directly implicated in the molecular mechanism of interferon action. We have cloned and sequenced following immunoscreening, a full-length cDNA that encodes the RNase L inhibitor (RLI). Northern blot analysis from a variety of human tissues revealed that two transcript forms (3.8 kb and 2.4 kb) are ubiquitously expressed but differences in levels of expression suggest a tissue-specific regulation. The RLI gene was localized to locus 4q31 by in situ hybridization indicating that this gene and other enzymes of the 2-5A pathway are not organized in cluster in the human genome.
Aubry
F
F
Laboratoire de Recombinaisons Génétiques - UPR 41 CNRS, Faculté de Médecine, Rennes, France.
Mattéi
M G
MG
Barque
J P
JP
Galibert
F
F
D018528:ATP-Binding Cassette Transporters; D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D018833:Chaperonins; D002871:Chromosome Banding; D002874:Chromosome Mapping; D002894:Chromosomes, Human, Pair 4; D018076:DNA, Complementary; D004722:Endoribonucleases / Q000037:antagonists & inhibitors; D004791:Enzyme Inhibitors; D005260:Female; D015894:Genome, Human; D006801:Humans; D017403:In Situ Hybridization; D008297:Male; D008969:Molecular Sequence Data; D009928:Organ Specificity; D011247:Pregnancy; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis*; D014158:Transcription, Genetic
C501917:ABCE1 protein, human; D018528:ATP-Binding Cassette Transporters; D018076:DNA, Complementary; D004791:Enzyme Inhibitors; D011506:Proteins; D012333:RNA, Messenger; D004722:Endoribonucleases; C031956:2-5A-dependent ribonuclease; D018833:Chaperonins