title: Assignment of the human alpha-tropomyosin gene TPM4 to band 19p13.1 by fluorescence in situ hybridization.
abstract: Sequence-tagged sites (STSs) were developed for the human alpha-tropomyosin gene TPM4. One STS was used to amplify DNA from somatic cell hybrids to localize TPM4 to chromosome 19. The other, a product from a long-range PCR, was used directly as a probe to refine the localization of TPM4 to 19p13.1 by fluorescence in situ hybridization to metaphase chromosome spreads.
authors.affiliation: Australian Neuromuscular Research Institute, QE II Medical Centre, Nedlands, Western Australia, Australia.
authors.forename: S D
authors.initials: SD
authors.lastname: Wilton
authors.forename: L
authors.initials: L
authors.lastname: Lim
authors.forename: S D
authors.initials: SD
authors.lastname: Dorosz
authors.forename: H C
authors.initials: HC
authors.lastname: Gunn
authors.forename: H J
authors.initials: HJ
authors.lastname: Eyre
authors.forename: D F
authors.initials: DF
authors.lastname: Callen
authors.forename: N G
authors.initials: NG
authors.lastname: Laing
mesh_terms: D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D002888:Chromosomes, Human, Pair 19 / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D015342:DNA Probes / Q000235:genetics; D006801:Humans; D006822:Hybrid Cells; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D016324:Sequence Tagged Sites; D014335:Tropomyosin / Q000235:genetics*
chemical_list: D017931:DNA Primers; D015342:DNA Probes; D014335:Tropomyosin