Carnosine sustains the retention of cell morphology in continuous fibroblast culture subjected to nutritional insult.
L- Carnosine (beta-alanyl L-histidine), occurring abundantly in skeletal muscles, has been suggested to possess antioxidant and anti-aging properties. Using three different experimental approaches (microscopic, flow cytometric and ELISA for one of the markers of DNA oxidative damage) this study on rat embryonic fibroblasts demonstrates that L-carnosine at 30 mM concentration sustains the retention of cell morphology even during a nutritional insult for five weeks. Also, L-carnosine significantly reduces the formation of 8-hydroxy deoxyguanosine (8-OH dG) in the cells after four weeks of continuous culture. Thus it could be inferred that the anti-senescent effect of L-carnosine is probably linked to its inhibition of formation of intracellular 8-OH dG during oxidative stress.
Kantha
S S
SS
Japan Institute for Control of Aging, Fukuroi City, Japan.
Wada
S
S
Tanaka
H
H
Takeuchi
M
M
Watabe
S
S
Ochi
H
H
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000818:Animals; D002336:Carnosine / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects*; D002999:Clone Cells; D046508:Culture Techniques / Q000379:methods; D004249:DNA Damage; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000032:analysis; D004622:Embryo, Mammalian; D004797:Enzyme-Linked Immunosorbent Assay; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D005434:Flow Cytometry; D008858:Microscopy, Phase-Contrast; D018384:Oxidative Stress; D051381:Rats; D011916:Rats, Inbred F344
D000080242:8-Hydroxy-2'-Deoxyguanosine; D002336:Carnosine; D003849:Deoxyguanosine