A cyclophilin gene-like sequence maps to human X-chromosome.
We isolated cDNA fragments encoded in an X-chromosome specific YAC from the locus DXS995 by using direct cDNA selection method. Several of the selected cDNA fragments were identical to various exons of cyclophilin A gene. Hybridization of the selected cyclophilin cDNA fragments to the target YAC, presence of these sequences in an X-chromosome specific phage library and absence of a cross hybridizing fragment in a cell line (XL-45) that contains deletions of the interval Xq21, demonstrates that a cyclophilin like sequence is present in the human X-chromosome.
Kandpal
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Fels Institute for Cancer Research and Molecular Biology, Temple University School of Medicine, Philadelphia, Pennsylvania 19140, USA.
Jacob
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Kandpal
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D000591:Amino Acid Isomerases / Q000235:genetics*; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D002460:Cell Line; D002872:Chromosome Deletion; D002874:Chromosome Mapping; D018244:Chromosomes, Artificial, Yeast; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D005091:Exons / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D019696:Peptidylprolyl Isomerase; D017422:Sequence Analysis, DNA; D014960:X Chromosome / Q000235:genetics*
D002352:Carrier Proteins; D018076:DNA, Complementary; D000591:Amino Acid Isomerases; D019696:Peptidylprolyl Isomerase