[Identification analysis using PCR of fresh or fixed and paraffin wax embedded tissue].
The aim of the work was a selection of the best method of DNA extraction from soft tissues, and than application of this method to the fixed and paraffin wax embedded tissue. Isolated DNA was amplified using PCR method. Amplification was done for amelogenin locus (sex specific sequences) and some polymorphic systems like mini- and microsatellites. It was found that it is possible to amplify 788 and 977 bp long amelogenin fragments, using DNA isolated from fixed and paraffin wax embedded tissue stored for two years. The case of identification of unknown person (victim of fire at Gdańsk Shipyard show room) on basis of DNA isolated from soft tissue was presented.
Pawłowski
R
R
Katedra i Zakład Medycyny Sadowej AM w Gdańsku.
D053523:Amelogenin; D004247:DNA / Q000032:analysis*; D003746:Dental Enamel Proteins / Q000235:genetics; D018732:Forensic Anthropology / Q000379:methods*; D006801:Humans; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction / Q000379:methods*; D016707:Tissue Fixation; D014083:Tooth Germ / Q000737:chemistry
D053523:Amelogenin; D003746:Dental Enamel Proteins; D004247:DNA
Badania identyfikacyjne metoda PCR tkanek swiezych oraz utrwalonych i zatopionych w blokach parafinowych.