Long-term lymphohematopoietic reconstitution by a single CD34-low/negative hematopoietic stem cell.
Hematopoietic stem cells (HSCs) supply all blood cells throughout life by making use of their self-renewal and multilineage differentiation capabilities. A monoclonal antibody raised to the mouse homolog of CD34 (mCD34) was used to purify mouse HSCs to near homogeneity. Unlike in humans, primitive adult mouse bone marrow HSCs were detected in the mCD34 low to negative fraction. Injection of a single mCD34(lo/-), c-Kit+, Sca-1(+), lineage markers negative (Lin-) cell resulted in long-term reconstitution of the lymphohematopoietic system in 21 percent of recipients. Thus, the purified HSC population should enable analysis of the self-renewal and multilineage differentiation of individual HSCs.
Osawa
M
M
Department of Immunology, Institute of Basic Medical Sciences and Center for Tsukuba Advanced Research Alliance, University of Tsukuba, Tsukuba Science-City, Ibaraki 305, Japan.
Hanada
K
K
Hamada
H
H
Nakauchi
H
H
D000818:Animals; D018952:Antigens, CD34 / Q000032:analysis*; D001483:Base Sequence; D001854:Bone Marrow Cells; D002454:Cell Differentiation; D019070:Cell Lineage; D002469:Cell Separation; D017931:DNA Primers; D006410:Hematopoiesis; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D013997:Time Factors
D018952:Antigens, CD34; D017931:DNA Primers