Interaction of mtTFB and mtRNA polymerase at core promoters for transcription of Xenopus laevis mtDNA.
Transcription of Xenopus laevis mitochondrial DNA requires mtRNA polymerase and a dissociable factor, xl-mtTFB, that is distinct from the HMG-box factor known as mtTFA. This paper presents the purification of mtTFB and characterizes its DNA binding properties. xl-mtTFB activity copurifies with a 40-kDa polypeptide on silver-stained protein gels. Activity can be recovered following elution of this 40-kDa polypeptide from an SDS-polyacrylamide gel. xl-mtTFB is capable of binding to DNA, but this binding is relatively nonspecific and is easily competed by heterologous DNA.
Bogenhagen
D F
DF
Department of Pharmacological Sciences, University of Stony Brook, New York 11794-8651, USA.
D000818:Animals; D004247:DNA / Q000378:metabolism; D004272:DNA, Mitochondrial / Q000235:genetics*; D012321:DNA-Directed RNA Polymerases / Q000302:isolation & purification / Q000378:metabolism*; D005260:Female; D011401:Promoter Regions, Genetic; D014157:Transcription Factors / Q000302:isolation & purification / Q000378:metabolism*; D014158:Transcription, Genetic; D014982:Xenopus laevis
D004272:DNA, Mitochondrial; D014157:Transcription Factors; D004247:DNA; D012321:DNA-Directed RNA Polymerases