A comparative analysis of plant mitochondrial small subunit ribosomal RNA sequences.
The small subunit of the ribosomal RNA has long been used as a tool in determining phylogenetic relationships. This project explored a region of the mitochondrial small subunit ribosomal RNA (MSrRNA) that is found only in the plant mitochondrial genome referred to as Variable Region 7 (V7). The V7 region of cauliflower and radish, both members of the Brassicaceae family, was amplified with the polymerase chain reaction, cloned into the expression vector PBSSK +, and sequenced. There was only a 0.3% sequences difference between the cauliflower and radish V7 region, thus suggesting that there will not be sequence variation in this region within a plant species. Cauliflower and radish V7 sequence was compared with the 6 other sequences of plant MSrRNA V7 region available: wheat, corn, oats, evening primrose, soybean and lupine. Based on percent difference between the V7 region sequences, a phylogenetic tree was constructed that supports the placement of these 8 species in Cronquist's morphologically based phylogenetic tree of flowering plants.
Glendening
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Department of Biological and Environmental Sciences, Morehead State University, KY 40351, USA.
Tuerk
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D001483:Base Sequence; D008928:Mitochondria / Q000737:chemistry*; D008969:Molecular Sequence Data; D010944:Plants / Q000235:genetics* / Q000648:ultrastructure*; D016133:Polymerase Chain Reaction; D012313:RNA / Q000737:chemistry*; D000077278:RNA, Mitochondrial; D018749:RNA, Plant / Q000737:chemistry*; D012335:RNA, Ribosomal / Q000737:chemistry*; D016415:Sequence Alignment
D000077278:RNA, Mitochondrial; D018749:RNA, Plant; D012335:RNA, Ribosomal; D012313:RNA