cDNA cloning and tissue-specific expression of the phosphatidylcholine transfer protein gene.
We have isolated a cDNA containing the complete coding sequence of bovine liver phosphatidylcholine transfer protein (PC-TP). The deduced amino acid sequence consists of 213 amino acid residues and is, except for a lysine instead of an arginine at position 167, identical to the sequence determined by Edman degradation [Akeroyd, Moonen, Westerman, Puyk and Wirtz (1981) Eur. J. Biochem. 114, 385-391]. A cDNA encoding amino acid residues 41-214 of mouse lung PC-TP was also isolated. The predicted amino acid sequence was 90% similar (81% identical) to the corresponding sequence of bovine liver PC-TP, demonstrating that PC-TP is conserved among mammalian species. By Southern blot analysis, evidence was obtained for the presence of a single bovine PC-TP-encoding gene. The expression of the PC-TP gene was determined during mouse embryonic development and in adult mouse tissues using an RNase protection assay. PC-TP RNA was present in embryos at all stages of development as early as the embryonic stem cell, suggesting a role for PC-TP in cell growth and differentiation. Towards the end of embryonic development, just before term, high levels of PC-TP RNA were found in the liver. This level was even higher 7 days post-term. In addition to adult liver, high levels of PC-TP RNA were also found in kidney and testis. The prominent presence of PC-TP in developing and adult liver is compatible with its proposed role in bile formation.
Geijtenbeek
T B
TB
Division of Molecular Biology, The Netherlands Cancer Institute, Amsterdam, The Netherlands.
Smith
A J
AJ
Borst
P
P
Wirtz
K W
KW
D000595:Amino Acid Sequence; D000727:Androgen-Binding Protein; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000096:biosynthesis*; D002417:Cattle; D003001:Cloning, Molecular; D017931:DNA Primers; D018076:DNA, Complementary; D005314:Embryonic and Fetal Development; D005260:Female; D015870:Gene Expression; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010713:Phosphatidylcholines / Q000378:metabolism; D049294:Phospholipid Transfer Proteins; D016133:Polymerase Chain Reaction; D060789:Prostatein; D051381:Rats; D060765:Secretoglobins; D017386:Sequence Homology, Amino Acid; D014598:Uteroglobin; D060788:Mammaglobin B
D000727:Androgen-Binding Protein; D002352:Carrier Proteins; D017931:DNA Primers; D018076:DNA, Complementary; D010713:Phosphatidylcholines; D049294:Phospholipid Transfer Proteins; D060789:Prostatein; D060765:Secretoglobins; D014598:Uteroglobin; C511484:Scgb1d2 protein, rat; C511483:Scgb1d4 protein, rat; C556468:Scgb2a1 protein, rat; D060788:Mammaglobin B