title: Improved cloning of antibody variable regions from hybridomas by an antisense-directed RNase H digestion of the P3-X63-Ag8.653 derived pseudogene mRNA.
authors.affiliation: Max-Planck-Institut für Biophysik, Frankfurt, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Ostermeier
authors.forename: H
authors.initials: H
authors.lastname: Michel
mesh_terms: D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular; D003576:Electron Transport Complex IV / Q000276:immunology; D006825:Hybridomas / Q000276:immunology*; D007135:Immunoglobulin Variable Region / Q000235:genetics*; D008969:Molecular Sequence Data; D011544:Pseudogenes; D016372:RNA, Antisense / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D016914:Ribonuclease H / Q000378:metabolism*
chemical_list: D000911:Antibodies, Monoclonal; D007135:Immunoglobulin Variable Region; D016372:RNA, Antisense; D012333:RNA, Messenger; D003576:Electron Transport Complex IV; D016914:Ribonuclease H