title: Photo-patterning of sensor surfaces with biomolecular structures: characterisation using AFM and fluorescence microscopy.
abstract: The miniaturisation of biosensors has resulted in the need to develop techniques for the high resolution patterning of different biological molecules onto surfaces. In this paper, we describe a procedure for the selective deposition of antibodies using biological self-assembly with photo-activation of a bound ligand, and we will detail methods which may subsequently be used to characterise the resultant biomolecular constructs.
authors.affiliation: Department of Electronics & Electrical Engineering, University of Glasgow, UK.
authors.forename: H
authors.initials: H
authors.lastname: Morgan
authors.forename: D J
authors.initials: DJ
authors.lastname: Pritchard
authors.forename: J M
authors.initials: JM
authors.lastname: Cooper
mesh_terms: D000911:Antibodies, Monoclonal; D001360:Avidin / Q000737:chemistry; D001386:Azides / Q000737:chemistry; D015374:Biosensing Techniques; D001710:Biotin / Q000031:analogs & derivatives / Q000737:chemistry; D018625:Microscopy, Atomic Force / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods; D010777:Photochemistry; D012822:Silicon Dioxide
chemical_list: D000911:Antibodies, Monoclonal; D001386:Azides; D001360:Avidin; D001710:Biotin; D012822:Silicon Dioxide; C045549:photobiotin