Real-time two-photon confocal microscopy using a femtosecond, amplified Ti:sapphire system.
The bilateral imaging approach known from confocal applications operating in the line mode was used to realize real-time two-photon imaging. It is shown that the sectioning inherent to two-photon imaging could be improved by the introduction of a confocal line aperture in the imaging path. Using a high-power, low-repetition-rate amplified Ti:sapphire system, various biological objects were visualized including live boar sperm.
Brakenhoff
G J
GJ
Department of Molecular Cell Biology, University of Amsterdam, The Netherlands.
Squier
J
J
Norris
T
T
Bliton
A C
AC
Wade
M H
MH
Athey
B
B
D000818:Animals; D007834:Lasers; D008297:Male; D008433:Mathematics; D018613:Microscopy, Confocal / Q000295:instrumentation / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods*; D011058:Pollen / Q000166:cytology*; D013094:Spermatozoa / Q000166:cytology*; D013552:Swine