Interleukin-6 enhances glucose transport in 3T3-L1 adipocytes.
To study the effect of interleukin-6 (IL-6) on glucose transport, 2-deoxy-D-glucose (2-DOG) uptake in 3T3-L1 adipocytes after incubation with IL-6 was measured. IL-6 increased 2-DOG uptake maximally after 5 hours by 30 +/- 12%, with a half-maximal effect at an IL-6 concentration of approximately 800 U/mL. 3-O-methylglucose uptake was stimulated in a similar way, indicating that IL-6 enhanced glucose transport rather than phosphorylation. IL-6-induced glucose transport was additive to the effect of insulin. Addition of cycloheximide did not affect the stimulatory action of IL-6, although cycloheximide itself had profound effects on basal glucose transport. We conclude that IL-6 may enhance glucose uptake by increasing GLUT-1 intrinsic activity.
Stouthard
J M
JM
Metabolic Research Group of the Department of Internal Medicine, Academic Medical Center, University of Amsterdam, The Netherlands.
Oude Elferink
R P
RP
Sauerwein
H P
HP
D019325:3-O-Methylglucose; D016475:3T3 Cells; D017667:Adipocytes / Q000378:metabolism*; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D003513:Cycloheximide / Q000494:pharmacology; D003847:Deoxyglucose / Q000378:metabolism; D005947:Glucose / Q000378:metabolism*; D007328:Insulin / Q000494:pharmacology; D015850:Interleukin-6 / Q000494:pharmacology*; D007700:Kinetics; D008757:Methylglucosides / Q000378:metabolism; D051379:Mice; D010766:Phosphorylation; D011994:Recombinant Proteins / Q000494:pharmacology
D007328:Insulin; D015850:Interleukin-6; D008757:Methylglucosides; D011994:Recombinant Proteins; D019325:3-O-Methylglucose; D003513:Cycloheximide; D003847:Deoxyglucose; D005947:Glucose