title: A novel expression vector for transfection of bovine MHC class I genes.
abstract: A vector is described for the expression of genomic or cDNA copies of bovine major histocompatibility complex (MHC) class I genes in transfected mouse Ltk- cells. Class I gene fragments are amplified by the polymerase chain reaction, using primers in conserved parts of exon 2 and the 3'-untranslated region of the gene. Amplified class I gene fragments can then be subcloned into the expression vector, pBoLA-21, which contains the necessary 5'- and 3'-sequences for correct expression. The vector was tested by subcloning and expressing genomic and cDNA clones.
authors.affiliation: Division of Molecular Biology, Roslin Institute, Midlothian, UK.
authors.forename: S M
authors.initials: SM
authors.lastname: Sawhney
authors.forename: J
authors.initials: J
authors.lastname: Hopkins
authors.forename: R L
authors.initials: RL
authors.lastname: Spooner
authors.forename: G C
authors.initials: GC
authors.lastname: Russell
mesh_terms: D000818:Animals; D001483:Base Sequence; D002417:Cattle / Q000235:genetics*; D003001:Cloning, Molecular; D017931:DNA Primers; D018076:DNA, Complementary / Q000096:biosynthesis; D005805:Genes, MHC Class I; D005822:Genetic Vectors / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D014162:Transfection / Q000379:methods*
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary