title: A 11.5-kb 5'-terminal cDNA sequence of chicken breast muscle connectin/titin reveals its Z line binding region.
abstract: A partial 5'-region cDNA (11.5 kb) of chicken breast muscle connectin/titin encoding 3752 amino acids was sequenced. The predicted amino acid sequence contains 31 immunoglobulin C2 motifs and 10 interdomains. The sequence suggests a skeletal muscle type of connectin isoforms. Immunoelectron microscopic studies using antisera raised against several products of the cDNA fragments expressed in E. coli revealed that a region of some 800 amino acids from the N terminus of connectin is involved in its binding to the Z line in a sarcomere.
authors.affiliation: Department of Biology, Faculty of Science, Chiba University, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Yajima
authors.forename: H
authors.initials: H
authors.lastname: Ohtsuka
authors.forename: Y
authors.initials: Y
authors.lastname: Kawamura
authors.forename: H
authors.initials: H
authors.lastname: Kume
authors.forename: T
authors.initials: T
authors.lastname: Murayama
authors.forename: H
authors.initials: H
authors.lastname: Abe
authors.forename: S
authors.initials: S
authors.lastname: Kimura
authors.forename: K
authors.initials: K
authors.lastname: Maruyama
mesh_terms: D000818:Animals; D001665:Binding Sites; D002352:Carrier Proteins / Q000096:biosynthesis; D002645:Chickens; D003001:Cloning, Molecular; D064211:Connectin; D018076:DNA, Complementary / Q000737:chemistry / Q000378:metabolism*; D004926:Escherichia coli; D015723:Gene Library; D005982:Glutathione Transferase; D016253:Microscopy, Immunoelectron; D009124:Muscle Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000648:ultrastructure; D018482:Muscle, Skeletal / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism; D011494:Protein Kinases / Q000096:biosynthesis* / Q000235:genetics / Q000648:ultrastructure; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure; D012518:Sarcomeres / Q000378:metabolism / Q000648:ultrastructure
chemical_list: D002352:Carrier Proteins; D064211:Connectin; D018076:DNA, Complementary; D009124:Muscle Proteins; D011993:Recombinant Fusion Proteins; D005982:Glutathione Transferase; D011494:Protein Kinases