Construction of a CUP1 promoter-based vector to modulate gene expression in Saccharomyces cerevisiae.
We report the construction and characterization of a yeast shuttle-expression vector that can be used to express genes in yeast in a modulated form. This vector is centromeric, with a polylinker that optionally can provide an ATG start of translation. Expression features are based on the CUP1 yeast metallothionein gene promoter, which can be tightly modulated by copper.
Mascorro-Gallardo
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JO
Departamento de Biología Molecular de Plantas, UNAM, Cuernavaca, Morelos, Mexico.
Covarrubias
A A
AA
Gaxiola
R
R
D001483:Base Sequence; D002352:Carrier Proteins; D003001:Cloning, Molecular; D004271:DNA, Fungal; D015966:Gene Expression Regulation, Fungal; D005822:Genetic Vectors; D008668:Metallothionein / Q000235:genetics*; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics*
C500769:CUP1-1 protein, S cerevisiae; D002352:Carrier Proteins; D004271:DNA, Fungal; C007121:copper thionein; D008668:Metallothionein