title: The pathogenesis of multicystic dysplastic kidney disease: insights from the study of fetal kidneys.
abstract: The pathogenesis of multicystic dysplastic kidney disease (MCDKD) is unknown. Most morphologic studies of MCDKD kidneys have been performed when the kidneys are resected postnatally, when their architecture has been distorted by massive cyst enlargement. We obtained two MCDKD kidneys at an early stage of development (14 and 19 weeks' gestation) and examined the pattern of nephrogenesis in detail. In both affected kidneys, we identified islands of spatially dislocated metanephric blastema adjacent to zones containing all the normal structural elements of nephrogenesis, including aggregates of induced mesenchyme, S-shaped bodies and maturing glomerull, and proximal and distal tubules. Metanephric blastemal cells displayed characteristic vimentin and smooth muscle actin immunoreactivity and insulin-like growth factor II gene expression, whereas induced elements exhibited appropriate cytokeratin immunoreactivity and Wilms' tumor gene expression. In most other zones, renal cysts were lined with epithelia varying from a flattened squamous to a cuboidal morphology and expression of markers suggested their origin to be from all portions of the nephron including Bowman's space, proximal tubule, and collecting duct. In some cysts, small clusters of epithelial cells were identified within the cyst lumen. These studies suggest that in the early stages of MCDKD, normal nephrogenesis occurs in what seems to be a normal metanephric blastema; however, an intrinsic abnormality in the branching morphogenesis of the ureteric duct might be responsible for the development of the histopathologic changes described.
authors.affiliation: Department of Pediatrics, University of Western Ontario, London, Canada.
authors.forename: D G
authors.initials: DG
authors.lastname: Matsell
authors.forename: T
authors.initials: T
authors.lastname: Bennett
authors.forename: P
authors.initials: P
authors.lastname: Goodyer
authors.forename: C
authors.initials: C
authors.lastname: Goodyer
authors.forename: V K
authors.initials: VK
authors.lastname: Han
mesh_terms: D000199:Actins / Q000032:analysis; D004268:DNA-Binding Proteins / Q000032:analysis / Q000235:genetics; D005315:Fetal Diseases / Q000473:pathology; D005333:Fetus; D016162:Genes, Wilms Tumor; D005865:Gestational Age; D006801:Humans; D007150:Immunohistochemistry; D007335:Insulin-Like Growth Factor II / Q000032:analysis / Q000235:genetics; D007633:Keratins / Q000032:analysis; D007668:Kidney / Q000737:chemistry / Q000196:embryology* / Q000473:pathology; D007678:Kidney Glomerulus / Q000737:chemistry / Q000196:embryology / Q000473:pathology; D007684:Kidney Tubules / Q000737:chemistry / Q000196:embryology / Q000473:pathology; D009024:Morphogenesis; D007690:Polycystic Kidney Diseases / Q000196:embryology* / Q000473:pathology*; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D014157:Transcription Factors / Q000032:analysis / Q000235:genetics; D014746:Vimentin / Q000032:analysis; D025721:WT1 Proteins
chemical_list: D000199:Actins; D004268:DNA-Binding Proteins; D012333:RNA, Messenger; D014157:Transcription Factors; D014746:Vimentin; D025721:WT1 Proteins; D007335:Insulin-Like Growth Factor II; D007633:Keratins