Herpes virus-like sequences are specifically found in Kaposi sarcoma lesions.
OBJECTIVE
To detect the prevalence of herpes virus-like DNA sequences in AIDS associated Kaposi sarcoma (KSHV) lesions and normal tissue.


METHODS
KSHV detection was performed by polymerase chain reaction (PCR) using four different sets of primers. PCR products were cloned, sequenced, and analysed.


RESULTS
All of four biopsies of Kaposi sarcoma lesions and all of three paraffin embedded Kaposi sarcoma tissues were positive for KSHV, while normal tissue from the same patients was negative. Sequence analysis of amplification products revealed polymorphisms that result in amino acid changes of the predicted sequence.


CONCLUSIONS
KSHV is prevalent in tissues from Kaposi sarcoma, suggesting a role in the development of the tumour. On this basis, anti-herpes virus agents should be considered to control Kaposi sarcoma.
O'Neill
E
E
Department of Virology/Molecular Biology, St Jude Children's Research Hospital, Memphis, TN 38105, USA.
Henson
T H
TH
Ghorbani
A J
AJ
Land
M A
MA
Webber
B L
BL
Garcia
J V
JV
D000163:Acquired Immunodeficiency Syndrome / Q000150:complications / Q000821:virology*; D000596:Amino Acids / Q000032:analysis; D001483:Base Sequence; D004279:DNA, Viral / Q000032:analysis; D006564:Herpesviridae / Q000302:isolation & purification*; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012514:Sarcoma, Kaposi / Q000209:etiology / Q000821:virology*
D000596:Amino Acids; D004279:DNA, Viral