title: Assessment of donor cell and matrix survival in fresh articular cartilage allografts in a goat model.
abstract: The long-term survival of allografts of articular cartilage has been proposed to be dependent on the survival of the cells that maintain the unique structural and material properties of the allograft. In this study, we assessed cell survival in 24 fresh articular cartilage allografts of the medial plateau in a Spanish-goat model. A DNA-probe technique was used to distinguish clearly between DNA from donor (allograft) and host cells. The intraarticular survival of viable allograft chondrocytes in the transplanted articular cartilage started to diminish as early as 3 weeks after transplantation; however, there was considerable variation in the amount of donor cell DNA detected in the allografts at 6 and 12 months following transplantation. This contrasts with our experience with fresh allografts of ligament, tendon, and meniscus, in which no donor DNA was detected 4 weeks after transplantation. DNA from host cells was present in all articular cartilage allografts, as evidenced by detectable unique host DNA patterns. Histological and histochemical assays showed that none of the transplants demonstrated normal structure and composition at 1 year after transplantation. The grafts in which large quantities of donor DNA were present appeared grossly superior to those with no or reduced remaining demonstrable donor DNA.
authors.affiliation: Orthopaedic Research Institute, Southern California Center for Sports Medicine, Long Beach, USA.
authors.forename: D W
authors.initials: DW
authors.lastname: Jackson
authors.forename: J
authors.initials: J
authors.lastname: Halbrecht
authors.forename: C
authors.initials: C
authors.lastname: Proctor
authors.forename: D
authors.initials: D
authors.lastname: Van Sickle
authors.forename: T M
authors.initials: TM
authors.lastname: Simon
mesh_terms: D000818:Animals; D002358:Cartilage, Articular / Q000737:chemistry / Q000166:cytology* / Q000637:transplantation*; D002470:Cell Survival; D004247:DNA / Q000032:analysis; D015342:DNA Probes; D005109:Extracellular Matrix / Q000737:chemistry; D006041:Goats; D006085:Graft Survival; D006651:Histocytochemistry; D014019:Tissue Donors; D014182:Transplantation, Autologous
chemical_list: D015342:DNA Probes; D004247:DNA