title: Human rotavirus VP4 contains strain-specific, serotype-specific and cross-reactive neutralization sites.
abstract: The neutralization epitopes of human rotavirus VP4 were studied by using a panel of neutralization monoclonal antibodies previously shown to be strain-specific (RV-3:3), serotype-specific (RV-5:2, ST-3:3) or cross-reactive (F45:4). Antigenic variants of human rotaviruses RV-3, ST-3, RV-5 and F45 resistant to neutralization by the appropriate of VP4 specific monoclonal antibodies (RV-3:3, ST-3:3, RV-5:2 and F45:4 respectively) were selected. By nucleotide sequence analysis and single strand conformational polymorphism analysis of these variants, three sites of neutralization on VP5* and one site on VP8* were identified. At or near to the putative fusion region on VP5*, a strain-specific site (aa383), a serotype P1A-P2 cross-reactive site (aa392) and a serotype P2-specific site (aa397) were found. On VP8*, a serotype P1B-specific site at aa148 was detected. These results confirmed the importance of the putative fusion region in neutralization and have identified a new neutralization site in the hypervariable region of VP8* which is specific for serotype P1B human rotaviruses.
authors.affiliation: Department of Gastroenterology, Royal Children's Hospital, Parkville, Victoria, Australia.
authors.forename: C D
authors.initials: CD
authors.lastname: Kirkwood
authors.forename: R F
authors.initials: RF
authors.lastname: Bishop
authors.forename: B S
authors.initials: BS
authors.lastname: Coulson
mesh_terms: D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology; D000956:Antigens, Viral; D001483:Base Sequence; D002213:Capsid / Q000276:immunology*; D036022:Capsid Proteins; D002460:Cell Line; D003429:Cross Reactions; D018604:Epitope Mapping; D014644:Genetic Variation; D006801:Humans; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D018807:Polymorphism, Single-Stranded Conformational; D012401:Rotavirus / Q000235:genetics / Q000276:immunology*; D012703:Serotyping
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D036022:Capsid Proteins; C060339:VP4 protein, Rotavirus; C050779:VP7 protein, Rotavirus