title: Catalase and superoxide dismutase activities as biomarkers of oxidative stress in workers exposed to mercury vapors.
abstract: For this article we investigated the role of three blood antioxidant enzyme activities and total antioxidant status (TAS) as biological markers of oxidative stress in workers exposed to mercury (Hg(o)) vapors. Twenty-two female workers took part in the study. The examination included a questionnaire on age, educational level, occupational history, actual health status, previous accidents and diseases, smoking and dietary habits, and alcohol consumption. Blood and urine sampling for biological analyses completed this examination. The workers were classified into three subgroups according to their creatinine-corrected Hg concentration in urine. Blood antioxidant enzyme activities and TAS were compared between groups with nonparametric distribution-free methods. A significant difference existed in catalase activity and a slight, but not significant, difference existed in Cu2+/Zn2+ superoxide dismutase (Cu2+/Zn2+ SOD) activity between the three groups. No differences were observed in either the glutathione peroxidase activity or the TAS between these groups. Catalase and Cu2+/Zn2+ SOD activities were increased in the groups of workers with higher creatinine-corrected urinary Hg concentrations when compared with the group of lower creatinine-corrected urinary Hg concentrations. Catalase activity was positively correlated with the creatinine-corrected concentration of Hg in urine, and Cu2+/Zn2+ SOD activity was slightly correlated with the creatinine-corrected concentration of Hg in urine. The role of erythrocyte catalase and Cu2+/Zn2+ SOD activities we have measured is in agreement with the hypothesis of the involvement of reactive oxygen species production as an important event in chronic exposure to Hg(o) vapors in humans. In spite of the small size of the sample, these results indicate that erythrocyte catalase and Cu2+/Zn2+ SOD activities could be considered as markers of biological effect in workers exposed to Hg(o) vapors.
authors.affiliation: INSERM U 420, Faculté de Médecine, Vandoeuvre-lès-Nancy, France. nadif@u420.nancy.inserm.fr
authors.forename: R
authors.initials: R
authors.lastname: Perrin-Nadif
authors.forename: M
authors.initials: M
authors.lastname: Dusch
authors.forename: C
authors.initials: C
authors.lastname: Koch
authors.forename: P
authors.initials: P
authors.lastname: Schmitt
authors.forename: J M
authors.initials: JM
authors.lastname: Mur
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000097:blood / Q000652:urine; D002374:Catalase / Q000097:blood* / Q000187:drug effects; D015331:Cohort Studies; D004912:Erythrocytes / Q000187:drug effects / Q000201:enzymology; D005260:Female; D005979:Glutathione Peroxidase / Q000097:blood / Q000187:drug effects; D006801:Humans; D008628:Mercury / Q000009:adverse effects* / Q000097:blood / Q000652:urine; D008630:Mercury Poisoning / Q000201:enzymology; D008875:Middle Aged; D016273:Occupational Exposure / Q000009:adverse effects*; D018384:Oxidative Stress / Q000502:physiology*; D012907:Smoking / Q000378:metabolism; D013482:Superoxide Dismutase / Q000097:blood* / Q000187:drug effects; D011795:Surveys and Questionnaires; D014835:Volatilization
chemical_list: D015415:Biomarkers; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D008628:Mercury