Structure and regional distribution of nociceptin/orphanin FQ precursor.
We have cloned cDNA carrying the entire coding sequence of the precursor protein for nociceptin/orphanin FQ, a neuropeptide-ligand for an opioid-receptor like G-protein coupled receptor. The deduced nociceptin/orphanin FQ precursor shows sequence similarity to the opioid peptide precursors and shares characteristic structural features particularly with preprodynorphin. In situ hybridization analysis of nociceptin precursor mRNA in the mouse central nervous system revealed that it is highly expressed in discrete neuronal sites with the pattern distinct from those of opioid peptides.
Houtani
T
T
Department of Anatomy, Kansai Medical University, Osaka, Japan.
Nishi
M
M
Takeshima
H
H
Nukada
T
T
Sugimoto
T
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000378:metabolism*; D003001:Cloning, Molecular; D017931:DNA Primers; D018076:DNA, Complementary; D004399:Dynorphins / Q000737:chemistry; D017403:In Situ Hybridization; D051379:Mice; D008969:Molecular Sequence Data; D009474:Neurons / Q000378:metabolism*; D018847:Opioid Peptides / Q000096:biosynthesis* / Q000737:chemistry; D009928:Organ Specificity; D016133:Polymerase Chain Reaction; D011498:Protein Precursors / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D051381:Rats; D011957:Receptors, Opioid / Q000819:agonists; D017386:Sequence Homology, Amino Acid; D013116:Spinal Cord / Q000378:metabolism*; D000097629:Nociceptin
D017931:DNA Primers; D018076:DNA, Complementary; D004399:Dynorphins; D018847:Opioid Peptides; D011498:Protein Precursors; D012333:RNA, Messenger; D011957:Receptors, Opioid; D000097629:Nociceptin