Liposomal vaccines.
Liposomes have been used therapeutically to deliver drugs to certain anatomical sites. The use of liposomes to deliver antigens, although not a new concept, has received less attention. At least two vaccines of nearly identical liposome base composition to our vaccines have been tested in humans. A malaria vaccine study showed that the liposomal preparation is quite safe: reaction profiles of volunteers receiving the vaccine demonstrated little reactivity and virtually no pyrogenicity (14). The concentration of MPLA in the vaccine was substantially higher (nearly 50,000 times) than the pyrogenic dose of free lipid A. The same vaccine, but different antigen (gp120, an HIV protein), was tested in volunteers and had the same lack of toxicity (27). In both studies, antibodies and cytotoxic cells specific for the respective antigens were produced. We have several subunit vaccines under development for infectious diseases (gram negative sepsis, fungal infections, protozoan infections), metabolic disorders (hypercholesterolemia, diabetic retinopathy, macular degeneration), and neoplastic diseases (multi-drug resistant cancer, primary and metastatic tumors, and angiogenic hyperproliferative disorders). In each case, one or more antigens were identified that might be useful in immunologic control of biologic proliferation (i.e., pathogen or tumor growth, rise in serum cholesterol, growth of blood vessels). We anticipate that at least one of these vaccines will be ready for testing in humans in the next calendar year.
Green
S
S
EntreMed, Inc., Rockville, Maryland 20850, USA.
Fortier
A
A
Dijkstra
J
J
Madsen
J
J
Swartz
G
G
Einck
L
L
Gubish
E
E
Nacy
C
C
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000276:immunology; D000276:Adjuvants, Immunologic; D000818:Animals; D000941:Antigens / Q000008:administration & dosage; D002784:Cholesterol / Q000276:immunology; D004337:Drug Carriers; D006801:Humans; D008081:Liposomes; D018805:Sepsis / Q000517:prevention & control; D014612:Vaccines / Q000008:administration & dosage*
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000276:Adjuvants, Immunologic; D000941:Antigens; D004337:Drug Carriers; D008081:Liposomes; D014612:Vaccines; D002784:Cholesterol