title: Heat-labile uracil-DNA glycosylase: purification and characterization.
abstract: A uracil-DNA glycosylase (UNG) from a psychrophilic marine bacterium (BMTU 3346) has been purified to apparent homogeneity. The enzyme has a molecular weight of 23400 Da. It is stable in complex buffers (containing glycerol/BSA), whereas it is heat-labile in dilute buffers (free of stabilizers) with a half-life of 2 min at 40 degrees C. Due to the thermolability, uracil-DNA glycosylase is suitable for application in the carryover prevention technique showing less residual activity and/or a slower reactivation rate than the usually applied UNG from Escherichia coli.
authors.affiliation: Boehringer Mannheim GmbH, Penzberg, Germany.
authors.forename: H
authors.initials: H
authors.lastname: Sobek
authors.forename: M
authors.initials: M
authors.lastname: Schmidt
authors.forename: B
authors.initials: B
authors.lastname: Frey
authors.forename: K
authors.initials: K
authors.lastname: Kaluza
mesh_terms: D001419:Bacteria / Q000201:enzymology; D001483:Base Sequence; D045647:DNA Glycosylases; D004795:Enzyme Stability; D006207:Half-Life; D006358:Hot Temperature; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009699:N-Glycosyl Hydrolases / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D051981:Uracil-DNA Glycosidase
chemical_list: D045647:DNA Glycosylases; D009699:N-Glycosyl Hydrolases; D051981:Uracil-DNA Glycosidase