Effect of 3-amino-1-propanol, indomethacin and 4-bromophenacyl bromide on the hormone binding of insulin pretreated Tetrahymena: influence of phospholipid metabolism disturbances on hormonal imprinting.
The compound 3-amino-1-propanol which replaces ethanolamine in phosphatidyl-ethanolamine and inhibits transformation of it, hindered insulin imprinting of Tetrahymena. Indomethacin, an inhibitor of prostaglandin synthesis and arachidonate metabolism provoked negative imprinting. 4-Bromophenacyl bromide, a phospholipase A2 inhibitor, hindered imprintability by insulin. Immediately after treatment these substances inhibited insulin binding. The experiment calls attention to the importance of intactness of metabolic pathways and second messenger systems in the mechanism of imprinting.
Kovács
P
P
Department of Biology, Semmelweis University of Medicine, Budapest, Hungary.
Csaba
G
G
D000098:Acetophenones / Q000494:pharmacology; D000818:Animals; D016861:Cyclooxygenase Inhibitors / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D016650:Fluorescein-5-isothiocyanate / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D007213:Indomethacin / Q000494:pharmacology; D007328:Insulin / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D010741:Phospholipases A / Q000037:antagonists & inhibitors; D054467:Phospholipases A2; D010743:Phospholipids / Q000378:metabolism*; D011412:Propanolamines / Q000494:pharmacology*; D013769:Tetrahymena pyriformis / Q000187:drug effects* / Q000378:metabolism
D000098:Acetophenones; D016861:Cyclooxygenase Inhibitors; D004791:Enzyme Inhibitors; D007328:Insulin; D010743:Phospholipids; D011412:Propanolamines; C031573:insulin, fluorescein-isothiocyanated-; D010741:Phospholipases A; D054467:Phospholipases A2; D016650:Fluorescein-5-isothiocyanate; C006821:4-bromophenacyl bromide; D007213:Indomethacin