Storage of noncryopreserved periphered blood stem cells for transplantation.
Mobilized peripheral blood stem cells (PBSC) were collected in autologous plasma and acid-citrate-dextrose formula A (ACD-A) by leukaphereses using the CS3000 cell separator (Baxter) and stored at 4 degrees C in a refrigerator for 8 days. We have looked at the viability of the nucleated cells with the trypan blue test and the proliferation and differentiation capacity using a standardized progenitor cell cloning assay. The changes in viability, granulocyte-macrophage colony-forming units (CFU-GM), erythroid burst-forming units (BFU-E), and mixed-lineage colony-forming units (CFU-GEMM) were determined daily during the storage period. Viability was 90.8% (SD 8%) at day 0 and declined to a mean of 69.5% (SD 15.5%) at day 8. CFU-GM decreased to 47% (SD 28.7%), CFU-GEMM to 48% (SD 42.2%), and BFU-E to 40.1% (SD 18.4%) after 6 days. After 5 days of storage the mean viability was 79.7% (SD 17.8%), whereas the mean CFU-GM were 65.3% (SD 28.4%) the mean CFU-GEMM were 61.8% (SD 30.4%) and the mean BFU-E were 55.1% (SD 18.2%). At day 4 viability was still 82.5% (SD 17.0%), recovery of CFU-GM was 78.5% (SD 28.8%), recovery of CFU-GEMM was 70.7% (SD 40.4%) and recovery of BFU-E was 65.0% (SD 17.5%). These data show, that PBSC can be stored safely over at least 5 days at 4 degrees C while the patient receives high-dose chemotherapy.
Hechler
G
G
Department of Hematology/Oncology, Philipps University Marburg, Germany.
Weide
R
R
Heymanns
J
J
Köppler
H
H
Havemann
K
K
D001781:Blood Component Removal; D001793:Blood Preservation; D002454:Cell Differentiation; D002455:Cell Division; D002470:Cell Survival; D002951:Citrates; D019343:Citric Acid; D015925:Cryopreservation; D015672:Erythroid Precursor Cells; D005947:Glucose / Q000031:analogs & derivatives; D006098:Granulocytes; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D007700:Kinetics; D008264:Macrophages; D013234:Stem Cells / Q000166:cytology / Q000502:physiology*
D002951:Citrates; C002113:acid citrate dextrose; D019343:Citric Acid; D005947:Glucose