title: Characterization and solubilization of testosterone 17 beta-hydroxysteroid dehydrogenase in human hyperplastic prostate.
abstract: 17 beta-Hydroxysteroid dehydrogenase is a membrane-bound enzyme in human prostate. Solubilization of this enzyme can only be obtained in the presence of detergents. The optimal solubilization mixture contained 50 mM Tris-HCl buffer pH 9.0, 20% glycerol, 0.1 M KCl and 5 mg/ml of the non-ionic detergent N-octyl glucoside. In these conditions, the soluble fraction contained more than 90% of the enzymatic activity. A 2.5-fold increase of specific activity was obtained during solubilization under optimal conditions.
authors.affiliation: Laboratoire de Biochimie, Faculté de Médecine, Brest, France.
authors.forename: J L
authors.initials: JL
authors.lastname: Carre
authors.forename: E
authors.initials: E
authors.lastname: Quemener
authors.forename: Y
authors.initials: Y
authors.lastname: Amet
authors.forename: B
authors.initials: B
authors.lastname: Simon
authors.forename: F
authors.initials: F
authors.lastname: Berthou
authors.forename: P
authors.initials: P
authors.lastname: Mangin
authors.forename: H H
authors.initials: HH
authors.lastname: Floch
authors.forename: J H
authors.initials: JH
authors.lastname: Abalain
mesh_terms: D015067:17-Hydroxysteroid Dehydrogenases / Q000737:chemistry / Q000378:metabolism*; D003902:Detergents; D006801:Humans; D008297:Male; D011467:Prostate / Q000201:enzymology*; D011470:Prostatic Hyperplasia / Q000201:enzymology*; D012995:Solubility; D013379:Substrate Specificity; D013739:Testosterone / Q000378:metabolism
chemical_list: D003902:Detergents; D013739:Testosterone; D015067:17-Hydroxysteroid Dehydrogenases; C044256:3 (or 17)-beta-hydroxysteroid dehydrogenase