A human chromosome 22 fosmid resource: mapping and analysis of 96 clones.
We have created a resource for chromosome 22 consisting of 96 unique, well-characterized Fosmids. The Fosmid vector permits efficient cloning of DNA fragments averaging 40 kb in a single-copy vector based on the F factor of Escherichia coli. We have found that Fosmid clones from human chromosome 22 show remarkable stability and are useful for a wide variety of applications in genome analysis. These 96 clones have been localized by FISH, using high-resolution fluorescent banding and multicolor mapping techniques, and their position on the chromosome was correlated with their content of a number of common repeated sequence elements. We identified a subset of clones likely to contain genes by restriction analysis using the enzymes NotI, MluI, SacII, and BssHII. This collection of cytogenetically anchored clones, representing nearly 7% of the chromosome, is of immediate value for detecting chromosomal rearrangements, for use in gene isolation, and as a framework for physical mapping.
Birren
B W
BW
Division of Biology 147-75, California Institute of Technology, Pasadena, California, 91125, USA.
Tachi-iri
Y
Y
Kim
U J
UJ
Nguyen
M
M
Shizuya
H
H
Korenberg
J R
JR
Simon
M I
MI
D001665:Binding Sites; D002503:Centromere / Q000737:chemistry / Q000235:genetics; D002874:Chromosome Mapping / Q000379:methods*; D002892:Chromosomes, Human, Pair 22; D003001:Cloning, Molecular; D004247:DNA / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005144:F Factor / Q000235:genetics; D005822:Genetic Vectors; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping
D004275:DNA, Ribosomal; D004247:DNA