Distribution of specific substance P binding sites in the heart and adjacent great vessels of the Wistar white rat.
Magnesium(Mg)-deficiency, whether dietary or an effect of a clinical condition such as diabetes, results in a variety of cardiovascular pathologies. Substance P (SP) has been implicated in the induction of cardiac focal inflammatory lesions that occur during Mg-deficiency. Blockade of SP receptors results in a significant reduction in the incidence of lesion formation. In an effort to identify potential endogenous cell populations of the heart, which may play a role in SP-dependent lesion formation, film- and light-microscopic autoradiography were used to map the distribution of specific SP binding sites in frozen sections of the normal rat heart and adjacent great vessels. Binding was assessed with 0.1 nM I-125 Bolton-Hunter labelled SP in the absence (total binding) or presence (non-specific binding) of excess unlabelled SP, prolactin, or L-703,606, a non-peptide antagonist of SP receptors. Film autoradiograms revealed prominent small foci of intense autoradiographic reactions dispersed intermittently around the periphery of the great vessels and coronary arteries, among the interstitial connective tissue of the heart, and along the cusps of the cardiac valves. Excess unlabelled SP caused a significant reduction (97.7% displacement; P < 0.001) in the focal autoradiographic reactions. L-703,606 caused a similar reduction in SP binding (97.3% displacement; P < 0.001), while prolactin had no statistically significant effect on the binding of radiolabelled SP. Light-microscopic autoradiograms revealed that the SP binding sites occurred within clusters of connective tissue cells or in rarely observed parasympathetic ganglia. No evidence was found to suggest the presence of SP receptors on endothelial cells, cardiac muscle fibers, or smooth muscle fibers. The connective tissue cells which bound SP within the heart will likely include types that are susceptible to SP activation and thus may play a role in initiation of the focal inflammation characteristic of Mg-deficiency.
Walsh
R J
RJ
Department of Anatomy, George Washington University Medical Center, Washington, D.C., USA.
Weglicki
W B
WB
Correa-de-Araujo
R
R
D000818:Animals; D001011:Aorta / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure; D001158:Arteries / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure; D001345:Autoradiography; D001665:Binding Sites; D009202:Cardiomyopathies / Q000378:metabolism; D003331:Coronary Vessels / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure; D006351:Heart Valves / Q000166:cytology; D008275:Magnesium Deficiency; D008297:Male; D018485:Muscle Fibers, Skeletal / Q000737:chemistry; D009131:Muscle, Smooth, Vascular / Q000737:chemistry; D009206:Myocardium / Q000737:chemistry* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D016622:Silver Staining; D013373:Substance P / Q000032:analysis* / Q000378:metabolism
D013373:Substance P