Recombination-dependent mutation in non-dividing cells.
Over the past 6 years an unexpected way of making mutations in bacteria has challenged concepts of the genetic mechanisms behind evolution. Mechanistic studies of these so called 'adaptive' mutations are revealing a novel molecular mechanism involving DNA double-strand breaks, genetic recombination, probable DNA polymerase errors, and the possible suspension of mismatch repair during the reversion of a lac frameshift mutation in Escherichia coli. The molecular details of this process are altering our understanding of how mutations form in non-dividing cells.
Rosenberg
S M
SM
Department of Biochemistry, University of Alberta Faculty of Medicine, Edmonton, Canada.
Harris
R S
RS
Longerich
S
S
Galloway
A M
AM
D000222:Adaptation, Physiological; D002455:Cell Division; D004249:DNA Damage / Q000235:genetics; D004260:DNA Repair; D004261:DNA Replication / Q000235:genetics; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D016368:Frameshift Mutation; D007763:Lac Operon; D008745:Methylation; D008957:Models, Genetic; D016296:Mutagenesis; D011995:Recombination, Genetic
D004259:DNA-Directed DNA Polymerase