Chromosomal assignment and imprinting tests for the mouse delta subunit of the cytosolic chaperonin containing TCP-1 (Cct4) gene to proximal chromosome 11.
The CCT (chaperonin containing TCP-1) complex functions as a molecular chaperone in the eukaryotic cytosol. This complex consists of several species of related polypeptides. The chromosomal localization of the mouse Cct4 gene encoding the delta subunit of CCT was assigned in this study to proximal chromosome 11 by genetic mapping. Restriction mapping analysis using YAC and pulse-field gel electrophoresis showed that Cct4 is located within a region about 300 kb from the imprinted gene U2af1-rs1. Expression of Cct4 was biallelic, and therefore Cct4 is not imprinted in neonatal mice. The localization of the human homologue of Cct4 on chromosome 2 corresponds well with the fact that homologues of other genes in the proximal region of mouse chromosome 11 also map to the region of conserved synteny in human chromosome 2.
Nabetani
A
A
Department of Bioscience, National Cardiovascular Center Research Institute, Suita, 565, Japan.
Hatada
I
I
Morisaki
H
H
Mukai
T
T
D000483:Alleles; D000818:Animals; D000831:Animals, Newborn; D001483:Base Sequence; D056404:Chaperonin Containing TCP-1; D018833:Chaperonins / Q000096:biosynthesis* / Q000235:genetics*; D002874:Chromosome Mapping; D002889:Chromosomes, Human, Pair 2; D003433:Crosses, Genetic; D003600:Cytosol / Q000378:metabolism; D017931:DNA Primers; D005260:Female; D015870:Gene Expression; D008040:Genetic Linkage; D018392:Genomic Imprinting; D006801:Humans; D046911:Macromolecular Substances; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D009115:Muridae; D016133:Polymerase Chain Reaction
C533300:CCT4 protein, human; D017931:DNA Primers; D046911:Macromolecular Substances; C533315:Tcp1 protein, mouse; D056404:Chaperonin Containing TCP-1; D018833:Chaperonins