title: Evaluation of ethanol on gastric epithelial restoration in vitro.
abstract: Ethanol exerts damaging effects on gastric mucosa and delays ulcer healing. To investigate the effect of ethanol on the wound repairing process, we used a wound repair model using primary cultured gastric mucosal cells. A confluent monolayer gastric mucosal cell sheet consisting mainly of mucous cells was wounded to make a cell-free area of constant size. Cell-free area was restored with time after wounding and monitored every 12 hr using a computer image analyzer to observe epithelial cell restoration quantitatively in the presence and absence of ethanol (2.0%). It was found that, although the control wound was completely repaired in 36 to 48 hr, the group treated with 2.0% ethanol showed a significant delay of repair. In the control, 5-bromodeoxyuridine-positive cells appeared around the wound in 24 to 36 hr. In contrast, the group treated with 2.0% ethanol showed no 5-bromodeoxyuridine-positive cells during the experiment. In conclusion, 2.0% ethanol retarded the repair of gastric mucosal restoration by inhibiting the initial gastric cell migration, followed by inhibition of proliferation of cells.
authors.affiliation: Department of Gastroenterology, Juntendo University School of Medicine, Tokyo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Murai
authors.forename: S
authors.initials: S
authors.lastname: Watanabe
authors.forename: M
authors.initials: M
authors.lastname: Hirose
authors.forename: O
authors.initials: O
authors.lastname: Kobayashi
authors.forename: K
authors.initials: K
authors.lastname: Maehiro
authors.forename: R
authors.initials: R
authors.lastname: Ohkura
authors.forename: H
authors.initials: H
authors.lastname: Miwa
authors.forename: T
authors.initials: T
authors.lastname: Kitamura
authors.forename: T
authors.initials: T
authors.lastname: Ogihara
authors.forename: H
authors.initials: H
authors.lastname: Oide
authors.forename: A
authors.initials: A
authors.lastname: Miyazaki
authors.forename: N
authors.initials: N
authors.lastname: Sato
mesh_terms: D000818:Animals; D002455:Cell Division / Q000187:drug effects / Q000502:physiology; D046508:Culture Techniques; D000431:Ethanol / Q000633:toxicity*; D005753:Gastric Mucosa / Q000473:pathology*; D007091:Image Processing, Computer-Assisted; D011817:Rabbits; D013276:Stomach Ulcer / Q000473:pathology*; D014945:Wound Healing / Q000187:drug effects* / Q000502:physiology
chemical_list: D000431:Ethanol