Identification of activities that catalyze the cis-trans isomerization of the double bond of a mono-unsaturated fatty acid in Pseudomonas sp. strain E-3.
A cell-free extract of Pseudomonas sp. strain E-3 catalyzed the conversion of 9-cis-hexadecenoic acid [16:1(9c)] to 9-trans-hexadecenoic acid [16:1(9t)] in the free acid form and when 16:1(9c) was esterified to phosphatidylethanolamine (PE). The cytosolic fraction catalyzed the isomerizations of free 16:1(9c) by itself and of 16:1(9c) esterified to PE in the presence of the membrane fraction. Tracer experiments using [2,2-2H2]16:1(9c) demonstrated that the isomerization of free 16:1(9c) occurred independently of the isomerization of 16:1(9c) esterified to PE, indicating that this bacterium has two types of activities that catalyze the cis-trans isomerization of the double bond of a mono-unsaturated fatty acid.
Okuyama
H
H
Laboratory of Environmental Molecular Biology, Graduate School of Environmental Earth Science, Hokkaido University, Kita-ku, Sapporo 060, Japan.
Enari
D
D
Shibahara
A
A
Yamamoto
K
K
Morita
N
N
D005231:Fatty Acids, Unsaturated / Q000378:metabolism*; D007535:Isomerases / Q000378:metabolism*; D010714:Phosphatidylethanolamines / Q000378:metabolism; D011549:Pseudomonas / Q000378:metabolism*
D005231:Fatty Acids, Unsaturated; D010714:Phosphatidylethanolamines; D007535:Isomerases