title: Pleomorphic adenoma and adenoid-cystic carcinoma of salivary glands: immunohistochemical assessment of proliferative activity in comparison with flow-cytometric study.
abstract: In this study, 32 pleomorphic adenomas (PAs) and seven adenoid cystic carcinomas (ACCs) were analysed for the evaluation of proliferating cell nuclear antigen (PCNA) indices and flow cytometric variables. Our aim was to assess any possible relationship between these parameters and the clinico-pathological variables and to clarify their histogenesis and reasons for their biological differences. The tumours were divided into three groups, mainly epithelial (E), myxoid (M) and chondroid (C); PCNA labelling index (LI) and weighted mean index (WI) and the WI/LI ratio were analysed in the predominant components; a single PCNA index, weighted by the percentage of each component, was also calculated. Only WI/LI was found to be significantly different in the three components, while PCNA single index did not show either significant differences by sex, age, site and size, or any correlation with the S phase fraction. A significant difference was found between PAs and ACCs by site (P < 0.01) and DNA ploidy (P < 0.05); furthermore, all PCNA indices (single index) were significantly lower in PAs than in ACCs.
authors.affiliation: Istituto di Anatomia ed Istologia Patologica, Palermo, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Daniele
authors.forename: V
authors.initials: V
authors.lastname: Tralongo
authors.forename: V
authors.initials: V
authors.lastname: Morello
authors.forename: C
authors.initials: C
authors.lastname: Nagar
authors.forename: A
authors.initials: A
authors.lastname: Russo
authors.forename: V
authors.initials: V
authors.lastname: Bazan
authors.forename: G
authors.initials: G
authors.lastname: Dardanoni
authors.forename: S
authors.initials: S
authors.lastname: Ciotta
authors.forename: R
authors.initials: R
authors.lastname: Nuara
authors.forename: R M
authors.initials: RM
authors.lastname: Tomasino
mesh_terms: D008949:Adenoma, Pleomorphic / Q000235:genetics / Q000503:physiopathology*; D000293:Adolescent; D000328:Adult; D000368:Aged; D000782:Aneuploidy; D003528:Carcinoma, Adenoid Cystic / Q000235:genetics / Q000503:physiopathology*; D002455:Cell Division / Q000502:physiology; D004273:DNA, Neoplasm / Q000032:analysis; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D011003:Ploidies; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D016196:S Phase / Q000502:physiology; D012468:Salivary Gland Neoplasms / Q000235:genetics / Q000503:physiopathology*
chemical_list: D004273:DNA, Neoplasm; D018809:Proliferating Cell Nuclear Antigen