Purification and properties of the first specific 5S rRNA binding protein from plants which shows transcription factor IIIA activity.
Eukaryotic transcription factor IIIA (TF IIIA) is know to activate specifically transcription of 5S rRNA gene. It also interacts with mature 5S rRNA. The best known TF IIIA has been purified from Xenopus laevis. Up to know this protein has not been isolated from plants. In this paper we show for the first time purification and properties of TF IIIA like protein from tulip (Tulipa whittalli). It shows 5S rRNA and 5S rRNA gene binding activity.
Wyszko
E
E
Institute of Biorganic Chemistry, Polish Academy of Sciences, Poznan, Poland.
Barciszewski
J
J
Barciszewska
M
M
D000818:Animals; D001665:Binding Sites; D004268:DNA-Binding Proteins / Q000302:isolation & purification* / Q000378:metabolism; D017343:Genes, Plant; D010940:Plant Proteins / Q000302:isolation & purification* / Q000378:metabolism; D010944:Plants / Q000235:genetics / Q000378:metabolism; D012341:RNA, Ribosomal, 5S / Q000235:genetics / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000302:isolation & purification* / Q000378:metabolism; D035582:Transcription Factor TFIIIA; D014157:Transcription Factors / Q000302:isolation & purification* / Q000378:metabolism; D014982:Xenopus laevis
D004268:DNA-Binding Proteins; D010940:Plant Proteins; D012341:RNA, Ribosomal, 5S; D016601:RNA-Binding Proteins; D035582:Transcription Factor TFIIIA; D014157:Transcription Factors