Nectrisine is a potent inhibitor of alpha-glucosidases, demonstrating activities similarly at enzyme and cellular levels.
Nectrisine, discovered as an immunomodulator, was found to inhibit alpha-glucosidase, alpha- and beta-mannosidases, beta-glucosidase and beta-N-acetylglucosaminidase, in that order of inhibition strength. Beta-Galactosidase, alpha-fucosidase, and neuraminidase were insensitive to this antibiotic. Also sensitive was the trimming glucosidase I which participates in the first step of modifying N-glycosidic oligosaccharide. Nectrisine demonstrated an inhibitory effect at the cellular level as strong as expected based on its action at enzyme levels; castanospermine and 1-deoxynojirimycin did not. Nectrisine and castanospermine suppressed syncytium formation and hemolytic activity in Newcastle disease virus (NDV)-infected BHK cells, without blocking the synthesis and cell-surface expression of HANA glycoprotein of NDV.
Tsujii
E
E
Animal and Cellular Systems Laboratory, The Institute of Physical and Chemical Research (RIKEN), Saitama, Japan.
Muroi
M
M
Shiragami
N
N
Takatsuki
A
A
D017485:1-Deoxynojirimycin / Q000494:pharmacology; D000118:Acetylglucosaminidase / Q000037:antagonists & inhibitors; D000276:Adjuvants, Immunologic; D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D015726:Giant Cells / Q000187:drug effects; D006023:Glycoproteins / Q000378:metabolism; D065089:Glycoside Hydrolase Inhibitors; D015707:HN Protein / Q000096:biosynthesis; D050113:Imino Furanoses; D007212:Indolizines / Q000494:pharmacology; D008361:Mannosidases / Q000037:antagonists & inhibitors; D011759:Pyrrolidines / Q000494:pharmacology*; D051381:Rats; D043323:alpha-Mannosidase; D001617:beta-Glucosidase / Q000037:antagonists & inhibitors; D044902:beta-Mannosidase; D054836:Indolizidines
D017485:1-Deoxynojirimycin; D000118:Acetylglucosaminidase; D000276:Adjuvants, Immunologic; D004791:Enzyme Inhibitors; D006023:Glycoproteins; D065089:Glycoside Hydrolase Inhibitors; D015707:HN Protein; D050113:Imino Furanoses; D007212:Indolizines; D008361:Mannosidases; D011759:Pyrrolidines; D043323:alpha-Mannosidase; D001617:beta-Glucosidase; D044902:beta-Mannosidase; C055825:nectrisine; C037806:castanospermine; D054836:Indolizidines