[Introduction of mutator phage D3112 of Pseudomonas aeruginosa into Alcaligenes eutrophus var. metallotollerans (Strain CH34)].
It is demonstrated that the intact genome of a D3112 tranposable phage (TP) of Pseudomonas aeruginosa, integrated into a recombinant plasmid RP4 :: D3112, can be transferred by means of conjugation from P. putida PpG1 (RP4npt :: D3112) donor cells into Alcaligenes eutrophus var. metallotollerans cells. P. aeruginosa strains are unacceptable as donors because they have a bactericidal effect on A. eutrophus. RP4npt :: D3112 plasmid is stably inherited by A. eutrophus with D3112 being expressed and successfully reproduced. However, TP loses the induction ability after UV irradiation or mitomycin C treatment. It is suggested that D3112 TP and its miniderivatives could be used in manipulations with A. eutrophus var. metallotolerans.
Krylov
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D000421:Alcaligenes / Q000821:virology*; D001435:Bacteriophages / Q000187:drug effects / Q000235:genetics* / Q000528:radiation effects; D003227:Conjugation, Genetic; D004251:DNA Transposable Elements; D015967:Gene Expression Regulation, Viral; D016679:Genome, Viral; D016685:Mitomycin / Q000494:pharmacology; D016254:Mutagenesis, Insertional; D010957:Plasmids; D011550:Pseudomonas aeruginosa / Q000821:virology*; D014466:Ultraviolet Rays
D004251:DNA Transposable Elements; D016685:Mitomycin
Vvedenie mutatornogo faga D3112 Pseudomonas aeruginosa v Alcaligenes eutrophus var. metallotollerans (strain CH34).