[The use of the polymerase chain reaction in the forensic medical study of hairs].
In order to detect polymorphic DNA sequences in the bulbs of single human hairs, polymerase chain reaction (PCR) was used. Specific hypervariable regions of ApoB gene, D1S80 locus, and sex-specific sequences of X/Y chromosomes were amplified. The amount of DNA isolated from single hair bulbs was measured by fluorometry. Methodological aspects of using PCR for forensic medical examinations of single hairs are discussed.
Puchkov
G F
GF
Kukhar'kov
Iu V
IuV
Korban
V V
VV
Borovko
S R
SR
D004247:DNA / Q000032:analysis / Q000302:isolation & purification; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005554:Forensic Medicine / Q000379:methods*; D006197:Hair / Q000737:chemistry*; D018859:Hair Follicle / Q000737:chemistry; D006257:Head; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods*; D012727:Sex Characteristics
D004247:DNA
Primenenie polimeraznoĭ tsepnoĭ reaktsii pri sudebno-meditsinskom issledovanii volos.