title: Genetic and phenotypic overlap between autophagy and the cytoplasm to vacuole protein targeting pathway.
abstract: We have explored the phenotypic and genetic overlap between autophagocytosis and cytoplasm to vacuole targeting in the yeast Saccharomyces cerevisiae. Complementation analysis was performed with mutants in each of these groups (aut and cvt, respectively), and three complementation groups were found to overlap. Also, most of the unique aut mutants accumulated precursor aminopeptidase I in the cytoplasm, while maintaining wild type kinetics and maturation of proteins targeted to the vacuole via the secretory pathway. The majority of the non-overlapping cvt mutants were found to be at least partially defective in autophagy. Some mutants in each group, however, appear to be only marginally affected in the other phenotype, implying that these pathways only partially overlap. We propose that import of aminopeptidase I into the vacuole shares a number of components required for bulk autophagocytosis, but is made specific, saturable, and constitutive by the presence of a receptor or other interacting protein(s).
authors.affiliation: Section of Microbiology, University of California, Davis, California 95616, USA.
authors.forename: T M
authors.initials: TM
authors.lastname: Harding
authors.forename: A
authors.initials: A
authors.lastname: Hefner-Gravink
authors.forename: M
authors.initials: M
authors.lastname: Thumm
authors.forename: D J
authors.initials: DJ
authors.lastname: Klionsky
mesh_terms: D000595:Amino Acid Sequence; D000626:Aminopeptidases / Q000378:metabolism*; D001343:Autophagy / Q000235:genetics*; D001692:Biological Transport; D002451:Cell Compartmentation; D003593:Cytoplasm / Q000201:enzymology / Q000378:metabolism*; D008969:Molecular Sequence Data; D009154:Mutation; D010641:Phenotype; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D014617:Vacuoles / Q000201:enzymology / Q000378:metabolism*
chemical_list: D029701:Saccharomyces cerevisiae Proteins; D000626:Aminopeptidases; C019689:APE1 protein, S cerevisiae