Evaluation of ethanol on gastric epithelial restoration in vitro.
Ethanol exerts damaging effects on gastric mucosa and delays ulcer healing. To investigate the effect of ethanol on the wound repairing process, we used a wound repair model using primary cultured gastric mucosal cells. A confluent monolayer gastric mucosal cell sheet consisting mainly of mucous cells was wounded to make a cell-free area of constant size. Cell-free area was restored with time after wounding and monitored every 12 hr using a computer image analyzer to observe epithelial cell restoration quantitatively in the presence and absence of ethanol (2.0%). It was found that, although the control wound was completely repaired in 36 to 48 hr, the group treated with 2.0% ethanol showed a significant delay of repair. In the control, 5-bromodeoxyuridine-positive cells appeared around the wound in 24 to 36 hr. In contrast, the group treated with 2.0% ethanol showed no 5-bromodeoxyuridine-positive cells during the experiment. In conclusion, 2.0% ethanol retarded the repair of gastric mucosal restoration by inhibiting the initial gastric cell migration, followed by inhibition of proliferation of cells.
Murai
T
T
Department of Gastroenterology, Juntendo University School of Medicine, Tokyo, Japan.
Watanabe
S
S
Hirose
M
M
Kobayashi
O
O
Maehiro
K
K
Ohkura
R
R
Miwa
H
H
Kitamura
T
T
Ogihara
T
T
Oide
H
H
Miyazaki
A
A
Sato
N
N
D000818:Animals; D002455:Cell Division / Q000187:drug effects / Q000502:physiology; D046508:Culture Techniques; D000431:Ethanol / Q000633:toxicity*; D005753:Gastric Mucosa / Q000473:pathology*; D007091:Image Processing, Computer-Assisted; D011817:Rabbits; D013276:Stomach Ulcer / Q000473:pathology*; D014945:Wound Healing / Q000187:drug effects* / Q000502:physiology
D000431:Ethanol