Natural antimutagenic agents.
Following a brief review of recent discoveries in the field of natural antimutagenic and tumor chemopreventive agents, contemporary findings in the author's laboratories employing the direct acting mutagen, ethyl methanesulfonate, in modified Ames tests and eukaryotic murine FM3A mammary tumor cells modified to be subject to thymidine-less death are described to illustrate the underlying principles. The EMS studies are illustrated with the isolation of the novel antimutagen, plicatin B, from the medicinal plants, Psoralea juncaea and P. plicata. The FM3A studies are carried out with extracts of Styrax asiatica, a plant previously studied extensively with the EMS system. The FM3A findings closely parallel the earlier work with EMS showing that the responsible agents, cinnamic acid, cinnamoyl ricinoleate and cinnamoyl cinnamate are effective both in prokaryotic and eukaryotic tests and that the new FM3A assay system has useful properties for screening and assay of novel antimutagenic agents.
Mitscher
L A
LA
Department of Medicinal Chemistry, Kansas University, Lawrence, USA.
Telikepalli
H
H
McGhee
E
E
Shankel
D M
DM
D000179:Acrylates / Q000302:isolation & purification / Q000494:pharmacology; D000818:Animals; D016588:Anticarcinogenic Agents / Q000494:pharmacology; D016587:Antimutagenic Agents / Q000737:chemistry / Q000494:pharmacology*; D004353:Drug Evaluation, Preclinical; D005020:Ethyl Methanesulfonate / Q000633:toxicity; D005260:Female; D008325:Mammary Neoplasms, Experimental; D051379:Mice; D008815:Mice, Inbred Strains; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity; D010936:Plant Extracts / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D000179:Acrylates; D016588:Anticarcinogenic Agents; D016587:Antimutagenic Agents; D009153:Mutagens; D010936:Plant Extracts; C098495:plicatin B acrylate; D005020:Ethyl Methanesulfonate