title: Phylogenetic analysis of the isopenicillin-N-synthetase horizontal gene transfer.
abstract: A phylogenetic study of the isopenicillin-N-synthetase (IPNS) gene sequence from prokaryotic and lower eukaryotic producers of beta-lactam antibiotics by means of a maximum-likelihood approach has been carried out. After performing an extensive search, rather than invoking a global molecular clock, the results obtained are best explained by a model with three rates of evolution. Grouped in decreasing order, these correspond to A. nidulans and then to the rest of the eukaryotes and prokaryotes, respectively. The estimated branching date between prokaryotic and fungal IPNS sequences (852 +/- 106 MY) strongly supports the hypothesis that the IPNS gene was horizontally transferred from bacterial beta-lactam producers to filamentous fungi.
authors.affiliation: Departamento de Genética, Facultad de Biología, Universidad de Valencia, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Buades
authors.forename: A
authors.initials: A
authors.lastname: Moya
mesh_terms: D001233:Aspergillus nidulans / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics*; D005656:Fungal Proteins / Q000235:genetics*; D006090:Gram-Negative Bacteria / Q000235:genetics; D006094:Gram-Positive Bacteria / Q000235:genetics; D016013:Likelihood Functions; D008957:Models, Genetic; D010088:Oxidoreductases / Q000235:genetics*; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012331:RNA, Fungal / Q000235:genetics; D012341:RNA, Ribosomal, 5S / Q000235:genetics; D016415:Sequence Alignment; D013045:Species Specificity; D013997:Time Factors; D014170:Transformation, Genetic
chemical_list: D001426:Bacterial Proteins; D005656:Fungal Proteins; D012329:RNA, Bacterial; D012331:RNA, Fungal; D012341:RNA, Ribosomal, 5S; D010088:Oxidoreductases; C038370:isopenicillin N synthetase