title: Protein engineering of a cephalosporin C acylase from Pseudomonas strain N176.
authors.affiliation: Pharmacological research Laboratories, Fujisawa Pharmaceutical Co., Ltd. Osaka, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Saito
authors.forename: Y
authors.initials: Y
authors.lastname: Ishii
authors.forename: T
authors.initials: T
authors.lastname: Fujimura
authors.forename: H
authors.initials: H
authors.lastname: Sasaki
authors.forename: Y
authors.initials: Y
authors.lastname: Noguchi
authors.forename: H
authors.initials: H
authors.lastname: Yamada
authors.forename: M
authors.initials: M
authors.lastname: Niwa
authors.forename: K
authors.initials: K
authors.lastname: Shimomura
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D001709:Biotechnology / Q000295:instrumentation / Q000379:methods; D005798:Genes, Bacterial; D005822:Genetic Vectors; D007700:Kinetics; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009838:Oligodeoxyribonucleotides; D010399:Penicillin Amidase / Q000096:biosynthesis* / Q000378:metabolism; D010957:Plasmids; D017354:Point Mutation; D015202:Protein Engineering / Q000295:instrumentation / Q000379:methods; D011549:Pseudomonas / Q000201:enzymology* / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000378:metabolism; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D013379:Substrate Specificity
chemical_list: D009838:Oligodeoxyribonucleotides; D011994:Recombinant Proteins; D010399:Penicillin Amidase