Hydrogen oxidation by membranes from autotrophically grown Alcaligenes eutrophus H16: role of the cyanide-resistant pathway in energy transduction.
Eighty percent of the ATP and proton electrochemical gradient (-ZDeltapH) formed during H2 oxidation in membranes from autotrophically grown exponential-phase cells of Alcaligenes eutrophus H16 was derived from a redox pathway that includes the membrane-bound hydrogenase complex and the cyanide-resistant (bo-type) oxidase. The H2/ubiquinone-1 oxidoreductase activity was coupled to energy transduction and was fully inhibited by the quinone along 2-n-hepthyl-4-hydroxyquinoline-N-oxide. We conclude that the cytochrome-c-containing pathway in exponential-phase A. eutrophus H16 cells plays a minor role in energy conservation.
Komen
R
R
Institut für Mikrobiologie der Georg-August Universität, Göttingen, D-37077 Göttingen, Germany.
Schmidt
K
K
Zannoni
D
D
D000421:Alcaligenes / Q000378:metabolism*; D004734:Energy Metabolism; D006859:Hydrogen / Q000378:metabolism*; D010084:Oxidation-Reduction; D011190:Potassium Cyanide / Q000494:pharmacology*
D006859:Hydrogen; D011190:Potassium Cyanide