Molecular cloning of sheep connexin49 and its identity with MP70.
The nucleotide sequence of the sheep homologue of the lens-specific mouse connexin50, chicken connexin45.6, and human connexin50 has been obtained following screening of a sheep genomic library. This connexin comprises 1323 nucleotides, coding for a protein of 440 amino acid residues and a predicted molecular weight of 49,160 daltons, so by convention is termed sheep connexin49. A connexin49 cDNA probe detected a single major band with a mobility of 6.8 kb in sheep lens RNA, but not in RNA isolated from five other sheep organs. The N-terminal amino acid sequence of sheep connexin49 is identical to that of mouse connexin50 and closely matches that of MP70, indicating the identity of sheep connexin49 with MP70. The nucleotide and translated amino acid sequences of connexin49 have 69-87% and 76%-87% identity respectively with chicken connexin45.6, human connexin50 and mouse connexin50. Like other members of this lens connexin family, sheep connexin49 coding region is completely contained within one exon, and the sequence of the N-terminal region, the four transmembrane domains and the two extracellular loops are highly conserved.
Yang
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DI
Department of Biochemistry, University of Minnesota, St Paul 55108, USA.
Louis
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CF
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002645:Chickens; D003001:Cloning, Molecular; D017630:Connexins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D004247:DNA / Q000032:analysis; D017931:DNA Primers / Q000737:chemistry; D015342:DNA Probes; D005136:Eye Proteins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D015698:Genomic Library; D006801:Humans; D007908:Lens, Crystalline / Q000737:chemistry*; D051379:Mice; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010766:Phosphorylation; D016133:Polymerase Chain Reaction; D012313:RNA / Q000302:isolation & purification; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D012756:Sheep; D014018:Tissue Distribution; D000099264:Connexin 50
D017630:Connexins; D004247:DNA; D017931:DNA Primers; D015342:DNA Probes; D005136:Eye Proteins; D012313:RNA; D000099264:Connexin 50