Cloning of a cDNA encoding a human homologue of CDC47, a member of the MCM family.
A complementary DNA (cDNA) clone encoding a 719-amino acid (aa) protein was isolated, which has a 49% aa identity with budding yeast CDC47, a member of the MCM family. Antiserum raised against a C-terminal polypeptide bacterially produced from the cDNA clone detected a cellular protein about 80 kDa, which coincides with the size of the in vitro transcription/translation product of the cDNA. These results indicate that the cDNA covers the entire coding region of a human homologue of CDC47.
Kiyono
T
T
Laboratory of Viral Oncology, Research Institute, Aichi Cancer Centre, Nagoya, Japan.
Fujita
M
M
Hayashi
Y
Y
Ishibashi
M
M
D000595:Amino Acid Sequence; D001483:Base Sequence; D018797:Cell Cycle Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D003001:Cloning, Molecular; D017124:Conserved Sequence; D018076:DNA, Complementary / Q000737:chemistry; D004268:DNA-Binding Proteins; D005800:Genes, Fungal; D006801:Humans; D064168:Minichromosome Maintenance Complex Component 7; D008969:Molecular Sequence Data; D009687:Nuclear Proteins; D010446:Peptide Fragments / Q000276:immunology; D010802:Phylogeny; D014176:Protein Biosynthesis; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic
D018797:Cell Cycle Proteins; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D010446:Peptide Fragments; C101624:MCM7 protein, human; D064168:Minichromosome Maintenance Complex Component 7