Altered biodistribution of an antibody--enzyme conjugate modified with polyethylene glycol.
Polyethylene glycol modification of the antibody--enzyme conjugate, F(ab')2-A5B7-CPG2, extends its duration in the circulation of nude mice bearing human colonic cancer xenografts (LS174T). Increased concentration of modified conjugate is achieved in the tumour, but residual non-specific enzyme concentrations in normal tissue and blood demonstrate the fundamental requirement to remove or inactivate non-specifically held enzyme in this system.
Eno-Amooquaye
E A
EA
Department of Medical Oncology, Charing Cross Hospital, London, UK.
Searle
F
F
Boden
J A
JA
Sharma
S K
SK
Burke
P J
PJ
D000818:Animals; D000911:Antibodies, Monoclonal; D002272:Carcinoembryonic Antigen / Q000276:immunology; D003110:Colonic Neoplasms / Q000097:blood / Q000378:metabolism*; D006801:Humans; D007140:Immunoglobulin Fab Fragments; D007457:Iodine Radioisotopes; D051379:Mice; D008819:Mice, Nude; D011092:Polyethylene Glycols; D011865:Radioisotope Dilution Technique; D012044:Regression Analysis; D013997:Time Factors; D014018:Tissue Distribution; D014183:Transplantation, Heterologous; D011623:gamma-Glutamyl Hydrolase / Q000493:pharmacokinetics*
D000911:Antibodies, Monoclonal; D002272:Carcinoembryonic Antigen; D007140:Immunoglobulin Fab Fragments; D007457:Iodine Radioisotopes; D011092:Polyethylene Glycols; D011623:gamma-Glutamyl Hydrolase