title: Detection of primary direct and indirect human anti-porcine T cell responses using a porcine dendritic cell population.
abstract: The pathways of human anti-pig T cell xenorecognition have been investigated. Freshly isolated porcine alveolar lavage (AL) cells induced primary proliferative responses by human peripheral and cord blood mononuclear cells which were inhibited by anti-HLA-DR antibody (indirect xenorecognition). Following over-night culture, the AL cells acquired the capacity to stimulate proliferation by purified human T cells which was inhibited by anti-SLA-DR antibody (direct xenorecognition). The marked increase in immunogenicity in the porcine AL cells was accompanied by a phenotypic change consistent with dendritic cell maturation. Limiting dilution assays indicate that the total anti-pig T cell response, in particular that mediated by indirect xenorecognition, is stronger than comparable alloresponses.
authors.affiliation: Department of Immunology, Royal Postgraduate Medical School, Hammersmith Hospital, London, GB.
authors.forename: A
authors.initials: A
authors.lastname: Dorling
authors.forename: G
authors.initials: G
authors.lastname: Lombardi
authors.forename: R
authors.initials: R
authors.lastname: Binns
authors.forename: R I
authors.initials: RI
authors.lastname: Lechler
mesh_terms: D000818:Animals; D015478:Antigens, Heterophile / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000502:physiology*; D006801:Humans; D013045:Species Specificity; D013552:Swine; D014183:Transplantation, Heterologous
chemical_list: D015478:Antigens, Heterophile