Increase of a type of oxidative DNA damage, 8-hydroxyguanine, and its repair activity in human leukocytes by cigarette smoking.
To investigate the oxidative stress induced by cigarette smoking, the levels of a form of oxidative DNA damage, 8-hydroxyguanine (8-OH-Gua), and its repair activity in the leukocytes of current smokers, ex-smokers, and complete nonsmokers were measured. The mean level of 8-OH-Gua was 1.88-fold higher in smokers as compared to complete nonsmokers (the difference was statistically significant, P = 0.013). The mean 8-OH-Gua repair activity was 1.6-fold higher in smokers than complete nonsmokers (the difference was statistically significant, P = 0.0053). A positive association was observed for the 8-OH-Gua levels and its repair activity. Considerable interindividual variations in the 8-OH-Gua levels (current smokers, 3.5-fold; nonsmokers, 7.2-fold) and in its repair activity (current smokers, 7.5-fold; nonsmokers. 5.5-fold) were observed. These results demonstrate that not only smoking status but also life-style, environment, and genetic differences might have effects on the level of 8-OH-Gua and its repair activity in human leukocytes.
Asami
S
S
Department of Environmental Oncology, University of Occupational and Environmental Health, Kitakyushu, Japan.
Hirano
T
T
Yamaguchi
R
R
Tomioka
Y
Y
Itoh
H
H
Kasai
H
H
D000328:Adult; D001483:Base Sequence; D004249:DNA Damage; D004260:DNA Repair; D004706:Endodeoxyribonucleases / Q000378:metabolism; D006147:Guanine / Q000031:analogs & derivatives* / Q000737:chemistry; D006801:Humans; D007962:Leukocytes / Q000737:chemistry; D008875:Middle Aged; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D010084:Oxidation-Reduction; D012907:Smoking
D009838:Oligodeoxyribonucleotides; C024829:8-hydroxyguanine; D006147:Guanine; D004706:Endodeoxyribonucleases