Cytokines and programmed cell death in burkitt lymphoma cells.
Tumour necrosis Factor (TNF), Interleukin 1alpha and beta (IL-alpha and IL-beta), Interleukin 7 (IL-7) and Stem Cell Factor (SCF) are cytokines synthesized by immune system cells under stimulation of various agents. Apoptosis, or programmed cell death, is a process that appears in response to specific stimuli, apparently following an intrinsic program. In this work we examined, in RA-1 human lymphoblastoid B cell line, the effect induced by different cytokines in cell proliferation and in programmed cell death. After 48 hours of treatment is present an antiproliferative affects, detected by 3H-thymidine incorporation and morphological changes related to apoptotic process.
Trubiani
O
O
Istituto di Morfologia Umana Normale, Università di Chieti, Italy.
Ciancarelli
M
M
Rapino
M
M
Di Primio
R
R
D017209:Apoptosis / Q000187:drug effects*; D002051:Burkitt Lymphoma / Q000188:drug therapy / Q000235:genetics / Q000473:pathology*; D002454:Cell Differentiation / Q000187:drug effects; D002843:Chromatin / Q000187:drug effects; D016207:Cytokines / Q000494:pharmacology*; D004586:Electrophoresis; D005434:Flow Cytometry; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology; D015851:Interleukin-7 / Q000494:pharmacology; D011994:Recombinant Proteins / Q000494:pharmacology; D019089:Stem Cell Factor / Q000494:pharmacology; D013936:Thymidine / Q000378:metabolism / Q000493:pharmacokinetics; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D002843:Chromatin; D016207:Cytokines; D007375:Interleukin-1; D015851:Interleukin-7; D011994:Recombinant Proteins; D019089:Stem Cell Factor; D014409:Tumor Necrosis Factor-alpha; D013936:Thymidine