In vitro import of pre-ferredoxin-NADP+-oxidoreductase from Cyanophora paradoxa into cyanelles and into pea chloroplasts.
Using a novel cyanelle isolation procedure we showed that pre-ferredoxin-NADP+-oxidoreductase (pre-FNR) from C. paradoxa is translocated in vitro across the peptidoglycan-containing cyanelle envelope. Efficient import was also observed in a heterologous system with pea chloroplasts as the recipient organelles. These results support the conclusion derived from comparative analysis of plastid genome organization, that all plastids originate from a common semi-autonomous endosymbiotic ancestor.
Jakowitsch
J
J
Institut für Biochemie und Molekulare Zellbiologie der Universität Wien und Ludwig-Boltzmann-Forschungsstelle für Biochemie, Austria.
Neumann-Spallart
C
C
Ma
Y
Y
Steiner
J
J
Schenk
H E
HE
Bohnert
H J
HJ
Löffelhardt
W
W
D002458:Cell Fractionation; D002498:Centrifugation; D002736:Chloroplasts / Q000378:metabolism*; D004792:Enzyme Precursors / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism*; D056890:Eukaryota / Q000201:enzymology* / Q000648:ultrastructure; D005287:Ferredoxin-NADP Reductase / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism*; D008970:Molecular Weight; D018532:Pisum sativum / Q000378:metabolism*; D010457:Peptidoglycan / Q000378:metabolism; D018087:Plastids / Q000201:enzymology* / Q000648:ultrastructure; D014158:Transcription, Genetic
D004792:Enzyme Precursors; D010457:Peptidoglycan; C098699:pre-ferredoxin-NADP-oxidoreductase; D005287:Ferredoxin-NADP Reductase