Isocratic high-performance liquid chromatographic determination of tryptophan in infant formulas.
The application to infant formulas of a method for tryptophan determination by isocratic HPLC with UV detection at 254 nm, after derivatization with phenyl isothiocyanate, was studied. Protein was hydrolysed by barium hydroxide at 120 degrees C for 8 h, followed by derivatization with phenyl isothiocyanate, HPLC and UV detection at 254 nm. The optimum chromatographic conditions (pH, ionic strength of elution solvent and eluent ratio) were established. The analytical parameters (linearity, precision, accuracy of derivatization and limits of detection and quantification) were determined. The values obtained demonstrated that the method is useful for determining the tryptophan content of infant formulas. The tryptophan contents in six different types of infant formulas were 0.535-0.848 g per 100 g of protein.
Alegría
A
A
Nutrition and Food Chemistry, University of Valencia, Burjassot, Spain.
Barberá
R
R
Farré
R
R
Ferrerés
M
M
Lagarda
M J
MJ
López
J C
JC
D000085:Acetates; D019342:Acetic Acid; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000706:statistics & numerical data; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007223:Infant; D007225:Infant Food / Q000032:analysis*; D007231:Infant, Newborn; D017879:Isothiocyanates; D009994:Osmolar Concentration; D012680:Sensitivity and Specificity; D012997:Solvents; D013861:Thiocyanates / Q000737:chemistry; D014364:Tryptophan / Q000032:analysis*
D000085:Acetates; D017879:Isothiocyanates; D012997:Solvents; D013861:Thiocyanates; C005441:phenylisothiocyanate; D014364:Tryptophan; D019342:Acetic Acid