title: The additional predictive value contributed by quantitative chromatin pattern description as compared to DNA ploidy level measurement in 257 superficial bladder transitional cell carcinomas.
abstract: The results of quantitative chromatin pattern description were compared with the determination of the DNA ploidy level, and the histological grading and clinical staging of superficial transitional cell carcinomas (sTCCs) of the bladder. We investigated whether this quantitative description adds useful information to the one obtained by the other methods used for predicting the biological behavior of sTCCs. The quantitative chromatin pattern description was carried out by means of the digital cell image analysis of Feulgen-stained nuclei in a series of 257 sTCCs. The results show that according to the clinical sequence "remission (134 patients) --> stable recurrence (101 patients) --> progression (13 patients) --> death (9 patients)', quantitative chromatin pattern description shows that certain areas of the nucleus undergo a continual process of condensation and that the distribution and spread of the chromatin becomes more and more heterogeneous. This is accompanied by a more frequent appearance of pale areas in the nucleus and an increase in nuclear size. Of the 257 sTCC patients, 148 had pTa/PpT1 grade I or III tumors. Of these 148, histological grading and clinical staging made it possible to correctly predict the biological behavior of the tumors of 120/148 (81%). DNA ploidy level determination increased this correct prediction from 81 to 84%. When the information contributed by quantitative chromatin pattern description was added to that contributed by the combination of histological grading, clinical staging and DNA ploidy level determination, the prediction level was 93%.
authors.affiliation: Department of Surgery, Institute J. Bordet, Brussels, Belgium.
authors.forename: R
authors.initials: R
authors.lastname: van Velthoven
authors.forename: M
authors.initials: M
authors.lastname: Petein
authors.forename: W J
authors.initials: WJ
authors.lastname: Oosterlinck
authors.forename: G
authors.initials: G
authors.lastname: Raviv
authors.forename: T
authors.initials: T
authors.lastname: Janssen
authors.forename: H
authors.initials: H
authors.lastname: Roels
authors.forename: J L
authors.initials: JL
authors.lastname: Pasteels
authors.forename: C
authors.initials: C
authors.lastname: Schulman
authors.forename: R
authors.initials: R
authors.lastname: Kiss
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000704:Analysis of Variance; D002295:Carcinoma, Transitional Cell / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D002467:Cell Nucleus / Q000473:pathology; D002843:Chromatin / Q000378:metabolism*; D015331:Cohort Studies; D004247:DNA / Q000378:metabolism*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local; D009365:Neoplasm Regression, Spontaneous; D009367:Neoplasm Staging; D011003:Ploidies; D011237:Predictive Value of Tests; D001749:Urinary Bladder Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology
chemical_list: D002843:Chromatin; D004247:DNA