title: The cDNA sequence and infectious transcripts of peanut stripe virus.
abstract: A full-length cDNA clone of the blotch isolate of the peanut stripe potyvirus (PStV) RNA genome was constructed downstream from the bacteriophage SP6 RNA polymerase promoter. The full-length PStV cDNA clone (PStVSF9) was sequenced and compared to the previously published sequence of PStV-B. In vitro-synthesized PStV transcripts capped with m7GpppG were infectious in Nicotiana benthamiana plants, and the progeny virus was aphid transmissible. To confirm the origin of infection, a mutant PStV (PStVDAE), with a Gly14 to Glu mutation in the coat protein-encoding gene, was constructed. Transcripts from PStVDAE produced symptoms indistinguishable from native or PStVSF9 virus, but was not transmitted by aphids.
authors.affiliation: Plant Biology Division, Samuel Roberts Noble Foundation, Ardmore, OK 73402, USA.
authors.forename: S
authors.initials: S
authors.lastname: Flasinski
authors.forename: U B
authors.initials: UB
authors.lastname: Gunasinghe
authors.forename: R A
authors.initials: RA
authors.lastname: Gonzales
authors.forename: B G
authors.initials: BG
authors.lastname: Cassidy
mesh_terms: D010367:Arachis / Q000821:virology*; D001483:Base Sequence; D002213:Capsid / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D008969:Molecular Sequence Data; D009154:Mutation; D017800:Potyvirus / Q000235:genetics* / Q000472:pathogenicity; D014158:Transcription, Genetic
chemical_list: D018076:DNA, Complementary