title: Antibodies to Haemophilus influenzae type b polysaccharide affect bacterial adherence and multiplication.
abstract: Since immunization of infants with conjugated Haemophilus influenzae type b (Hib) capsular polysaccharide (PS) vaccines results in a reduction of colonization, we determined the inhibitory effect of anti-Hib PS on two steps in the colonization, i.e., adherence of H. influenzae to nasopharyngeal epithelium and bacterial growth. Monoclonal antibody (MAb) E117-5 specific for Hib PS inhibited at a concentration of at least 80 microg/ml in vitro the adherence of Hib strain 770235f+b+ to oropharyngeal epithelial cells by 50% (P <, 0.02), but this MAb and sera from children immunized with Hib PS conjugate vaccine (n = 10) were not inhibitory in final dilutions containing up to 20 microg of anti-Hib PS per ml. The growth of Hib strain 770235f+b+ did completely stop in the presence of 5 microg of anti-Hib PS MAb E117-5 per ml and human sera with an anti-Hib PS concentration of 2 microg/ml or more, in contrast to the growth of the nonencapsular variant strain 770235f+b0.
authors.affiliation: Department of Medical Microbiology, University of Amsterdam, The Netherlands.
authors.forename: L
authors.initials: L
authors.lastname: van Alphen
authors.forename: P
authors.initials: P
authors.lastname: Eijk
authors.forename: H
authors.initials: H
authors.lastname: Käyhty
authors.forename: J
authors.initials: J
authors.lastname: van Marle
authors.forename: J
authors.initials: J
authors.lastname: Dankert
mesh_terms: D000371:Agglutination; D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology*; D001422:Bacterial Adhesion; D016667:Bacterial Capsules; D010861:Fimbriae, Bacterial / Q000502:physiology; D018073:Haemophilus Vaccines / Q000276:immunology*; D006193:Haemophilus influenzae / Q000254:growth & development / Q000276:immunology*; D006801:Humans; D051379:Mice; D009960:Oropharynx / Q000382:microbiology; D011135:Polysaccharides, Bacterial / Q000276:immunology*; D014407:Tumor Cells, Cultured
chemical_list: D000907:Antibodies, Bacterial; D018073:Haemophilus Vaccines; C044237:Haemophilus influenzae type b polysaccharide vaccine; D011135:Polysaccharides, Bacterial