The X-ray contrast medium iodixanol detects increased colonic permeability equally well as 51Cr-labeled ethylenediaminetetraacetic acid in experimental colitis of rats.
BACKGROUND
Non-ionic, water-soluble radiographic contrast media have been suggested as intestinal permeability probes. We studied the permeability of the isosmolar contrast medium iodixanol and 51Cr-labeled ethylenediaminetetraacetic acid (EDTA) from the non-perforated colon after induction of colonic inflammation.


METHODS
Colonic inflammation and ulcerations were induced by luminal colonic instillation of trinitrobenzenesulfonic acid, dissolved in 40% ethanol. Controls received saline. Fourteen days later iodixanol, 320 mg I/ml, and 51Cr-EDTA were given as an enema. Urine was collected for the subsequent 6 h and subjected to high-performance liquid chromatography and gamma activity counting.


RESULTS
Urinary recovery of iodixanol and 51Cr-EDTA increased gradually with severity of the colonic inflammation. The correlation between iodixanol and 51Cr-EDTA recovery was strong (corr.coeff = 0.97).


CONCLUSIONS
Iodixanol shows as good properties as 51Cr-EDTA when used as intestinal permeability probe in the inflamed and ulcerated rat colon. Use of the radiopaque properties of iodixanol enable intestinal probe exposure registration by film or fluoroscopy.
Andersen
R
R
Institute for Surgical Research, National Hospital, Oslo, Norway.
Hosaka
J
J
Halstensen
T S
TS
Stordahl
A
A
Tverdal
A
A
Aabakken
L
L
Laerum
F
F
D000818:Animals; D002463:Cell Membrane Permeability / Q000502:physiology*; D002860:Chromium Radioisotopes / Q000493:pharmacokinetics; D003092:Colitis / Q000139:chemically induced / Q000503:physiopathology*; D003287:Contrast Media / Q000493:pharmacokinetics; D004492:Edetic Acid / Q000493:pharmacokinetics; D005119:Extravasation of Diagnostic and Therapeutic Materials / Q000503:physiopathology*; D007408:Intestinal Absorption / Q000502:physiology*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D014283:Triiodobenzoic Acids / Q000493:pharmacokinetics; D014302:Trinitrobenzenesulfonic Acid / Q000633:toxicity
D002860:Chromium Radioisotopes; D003287:Contrast Media; D014283:Triiodobenzoic Acids; D014302:Trinitrobenzenesulfonic Acid; D004492:Edetic Acid; C044834:iodixanol