Peroxisome proliferators activate cytochrome P450 genes in an alkane-assimilating yeast, Candida maltosa.
Candida maltosa can assimilate n-alkane as a sole carbon source and cytochromes P450ALK (P450ALK) are critical for the first oxidation step. Four major P450ALKs that are encoded by genes ALK1, ALK2, ALK3 and ALK5 are induced by n-alkane and repressed by glucose at the transcriptional level. In the present work, we found that all these four genes but ALK5 are transcriptionally activated in response to a peroxisome proliferator, clofibrate. This is the first report on the peroxisome proliferator responsive gene expression in lower eucaryotes.
Ohtomo
R
R
Department of Biotechnology, University of Tokyo, Japan.
Kobayashi
K
K
Muraoka
S
S
Ohkuma
M
M
Ohta
A
A
Takagi
M
M
D000473:Alkanes / Q000378:metabolism; D002175:Candida / Q000201:enzymology*; D002273:Carcinogens / Q000494:pharmacology; D002994:Clofibrate / Q000494:pharmacology*; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D005656:Fungal Proteins; D015971:Gene Expression Regulation, Enzymologic; D015966:Gene Expression Regulation, Fungal; D008830:Microbodies / Q000187:drug effects; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics; D014158:Transcription, Genetic / Q000187:drug effects
D000473:Alkanes; D002273:Carcinogens; D005656:Fungal Proteins; D012333:RNA, Messenger; D003577:Cytochrome P-450 Enzyme System; C499277:Alk2 protein, Candida; D002994:Clofibrate