CD40/CD40L interactions and cytokines regulate HIV replication in B cells in vitro.
Using our in vitro model of normal B cell infection, we investigated whether cellular interactions and/or cytokines directing the B cell response also regulate HIV replication. Phorbol esters and CD40 Ab plus IL4, added prior to infection, substantially increased subsequent viral replication. Postinfection, IL2 with or without IL4 and, to a lesser extent, CD40/CD40L interactions enhanced viral replication. In contrast, IL10 down-regulated HIV replication induced by cytokines, without affecting spontaneous or CD40 Ab-induced replication. Both enhancing and inhibitory effects of cytokines on viral replication were independent of their ability to modulate B cell proliferation. Thus, these two phenomena seem to be independently regulated in human B cells.
Gras
G
G
Service de Neurovirologie, CEA/CRSSA, Institut Paris-Sud sur les Cytokines, Fontenay aux Roses, France.
Legendre
C
C
Krzysiek
R
R
Dormont
D
D
Galanaud
P
P
Richard
Y
Y
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology / Q000821:virology*; D019013:CD40 Antigens / Q000276:immunology*; D023201:CD40 Ligand; D002455:Cell Division; D002478:Cells, Cultured; D002648:Child; D016207:Cytokines / Q000276:immunology*; D015536:Down-Regulation; D016655:HIV Core Protein p24 / Q000096:biosynthesis; D015497:HIV-1 / Q000276:immunology / Q000502:physiology*; D006801:Humans; D016753:Interleukin-10 / Q000276:immunology; D007376:Interleukin-2 / Q000276:immunology; D015847:Interleukin-4 / Q000276:immunology; D008562:Membrane Glycoproteins / Q000276:immunology*; D012756:Sheep; D013601:T-Lymphocytes / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D014779:Virus Replication
D019013:CD40 Antigens; D016207:Cytokines; D016655:HIV Core Protein p24; D007376:Interleukin-2; D008562:Membrane Glycoproteins; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D023201:CD40 Ligand; D015847:Interleukin-4