Molecular cloning of the forkhead transcription factor HNF-3 alpha from a human pulmonary adenocarcinoma cell line.
We have cloned the human homologue of HNF-3 alpha from the pulmonary adenocarcinoma cell line NCI-H441. Full-length HNF-3 alpha which was found to be 2872 bp encoded a protein of 473 amino acids. The human protein is highly related to the rat and mouse homologues and the forkhead DNA binding domain is highly conserved between all species yet identified. Northern blotting with a variety of human-derived cell lines identified a single 3 kb mRNA species. RNA levels are > 10-fold higher in the H441 cells than in any of the others.
Bingle
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DH Department of Toxicology, St Bartholomew's School of Medicine, London, UK.
Gowan
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S
D000230:Adenocarcinoma / Q000737:chemistry / Q000378:metabolism / Q000473:pathology; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D017124:Conserved Sequence; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics*; D051541:Hepatocyte Nuclear Factor 3-alpha; D006801:Humans; D008175:Lung Neoplasms / Q000737:chemistry / Q000378:metabolism / Q000473:pathology; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors; D014407:Tumor Cells, Cultured
D004268:DNA-Binding Proteins; C496499:FOXA1 protein, human; D051541:Hepatocyte Nuclear Factor 3-alpha; D009687:Nuclear Proteins; D014157:Transcription Factors