Induction of morphological and functional differentiation of human promyelocytic leukemia cells (HL-60) by Tubeimoside 1.
Two functional parameters evaluated by nitroblue tetrazolium (NBT) dye reduction and zymosan particle phagocytosis and a morphological change assessed by the Wright-Giemsa stain of human promyelocytic leukemia cells (HL-60 cells) were used to determine the extent of HL-60 cell differentiation induced by tubeimoside 1. The results revealed that under the action of tubeimoside 1, HL-60 cells could be induced to differentiate to more mature cells with the functional characteristics of granulocytes. Therefore, tubeimoside 1 could be considered as a promising antileukemic agent for further animal trials.
Yu
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Department of Biochemistry, Shaanxi Provincial Academy of Traditional Chinese Medicine and Pharmacology, Xian, People's Republic of China.
Ma
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Yu
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D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects; D004365:Drugs, Chinese Herbal; D018922:HL-60 Cells; D006801:Humans; D012503:Saponins / Q000302:isolation & purification / Q000494:pharmacology*; D014315:Triterpenes / Q000302:isolation & purification / Q000494:pharmacology*
D000972:Antineoplastic Agents, Phytogenic; D004365:Drugs, Chinese Herbal; D012503:Saponins; D014315:Triterpenes; C051084:tubeimoside I