Interaction of alkanols and local anesthetics with spin-labeled Ca(2+)-ATPase of sarcoplasmic reticulum vesicles.
Alkanols and tertiary amine derivative local anesthetics modify the activity of Ca(2+)-ATPase. In order to investigate the primary binding sites, associated to the functional changes, sarcoplasmic reticulum (SR) Ca(2+)-ATPase was labeled with maleimide derivative spin labels which bind covalently to SH groups of cysteine residues and allow to probe the regions of the protein close to those residues. The EPR measurements showed motional constraints induced by drug-treatment which indicate changes in the enzyme dynamics and structure. n-Alkanols are shown to affect some of the protein-bound labels by restricting their motion. There is, however, no correlation between the functional effects and the observed motional restriction, in the sense that concentrations of the different alcohols leading to the same functional effects do not induce the same degree of restriction. Dibucaine and tetracaine at functional relevant concentrations also restrict the movement of protein bound labels. But, in this case, correlation between spectral changes and functional effects is observed.
Anteneodo
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Centro Brasileiro de Pesquisas Físicas, Rio de Janeiro, Brazil.
Louro
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SR
Wajnberg
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D020001:1-Butanol; D000779:Anesthetics, Local / Q000494:pharmacology*; D000818:Animals; D000440:Butanols / Q000494:pharmacology*; D000252:Calcium-Transporting ATPases / Q000378:metabolism*; D003992:Dibucaine / Q000494:pharmacology; D004578:Electron Spin Resonance Spectroscopy; D006863:Hydrogen-Ion Concentration; D008301:Maleimides; D018482:Muscle, Skeletal / Q000201:enzymology*; D011817:Rabbits; D012519:Sarcoplasmic Reticulum / Q000201:enzymology*; D013113:Spin Labels; D013748:Tetracaine / Q000494:pharmacology
D000779:Anesthetics, Local; D000440:Butanols; D008301:Maleimides; D013113:Spin Labels; D013748:Tetracaine; C043592:maleimide; D020001:1-Butanol; D000252:Calcium-Transporting ATPases; D003992:Dibucaine