Direct database searching with MALDI-PSD spectra of peptides.
The analysis of matrix-assisted laser desorption ionization post-source decay (MALDI-PSD) mass spectra of peptides by using the cross-correlation method for database searching is illustrated. MALDI-PSD mass spectra are shown to contain sufficient fragmentation information to uniquely identify the correct amino acid sequence from large protein databases (approximately 160,000 entries). A search employing the MALDI-PSD mass spectrum of a phosphorylated peptide that correctly identifies the amino acid sequence and the site of phosphorylation is also illustrated.
Griffin
P R
PR
Department of Inflammation Research, Merck Research Laboratories, Rahway, NJ 07065-0900, USA.
MacCoss
M J
MJ
Eng
J K
JK
Blevins
R A
RA
Aaronson
J S
JS
Yates
J R
JR
D000595:Amino Acid Sequence; D016208:Databases, Factual; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D008969:Molecular Sequence Data; D010455:Peptides / Q000032:analysis*; D010748:Phosphopeptides / Q000032:analysis
D010455:Peptides; D010748:Phosphopeptides