title: Elevated levels of homologous DNA recombination activity in the regenerating rat liver.
abstract: We have characterized homologous DNA recombination activity in nuclear protein extracts prepared from quiescent and regenerating rat livers. Activity measured in regenerating liver extracts was elevated approximately 35-fold above control, and its appearance closely mirrored the first wave of DNA synthesis, peaking 24 hours after a regenerative stimulus, and returning fairly rapidly to basal levels. We also identified a strand-transferase protein of approximately 100 kDa whose presence in these extracts correlates with homologous recombination activity. Recent evidence suggests that mammalian somatic cells possess a recombinational DNA repair mechanism analogous to that described in the yeast Saccharomyces cerevisiae. Our results indicate that this recombinational repair process may be regulated in vivo by, or play a role in, progression through the cell division cycle.
authors.affiliation: Department of Pharmacology, University of Minnesota Medical School, Minneapolis 55455, USA.
authors.forename: B
authors.initials: B
authors.lastname: Thyagarajan
authors.forename: J L
authors.initials: JL
authors.lastname: Cruise
authors.forename: C
authors.initials: C
authors.lastname: Campbell
mesh_terms: D000818:Animals; D002457:Cell Extracts; D002467:Cell Nucleus / Q000378:metabolism; D004247:DNA / Q000096:biosynthesis / Q000235:genetics; D004254:DNA Nucleotidyltransferases / Q000737:chemistry; D004260:DNA Repair / Q000502:physiology; D005260:Female; D008115:Liver Regeneration / Q000235:genetics*; D008970:Molecular Weight; D051381:Rats; D011916:Rats, Inbred F344; D011995:Recombination, Genetic
chemical_list: D002457:Cell Extracts; D004247:DNA; D004254:DNA Nucleotidyltransferases