Nerve growth factor (NGF)-mediated protection of neural crest cells from antimitotic agent-induced apoptosis: the role of the low-affinity NGF receptor.
Prevention by nerve growth factor (NGF) of apoptotic death in neural cells has been variously ascribed to binding of NGF to its low-affinity (p75) or high-affinity (trkA) receptor or to a cooperative interaction between the two. In a series of studies using, in turn, neuroblastoma cell lines that express only p75, mutant NGF species that bind selectively to either p75 or trkA, and a polyclonal antibody that binds to the NGF-binding domain of p75, we demonstrate that NGF binding to p75 is both necessary and sufficient for the abrogation of apoptosis in neuroblastoma cells treated with antimitotic agents.
Cortazzo
M H
MH
Department of Pediatrics, University of Pittsburgh, Pennsylvania 15213, USA.
Kassis
E S
ES
Sproul
K A
KA
Schor
N F
NF
D000970:Antineoplastic Agents / Q000494:pharmacology; D017209:Apoptosis / Q000502:physiology; D001426:Bacterial Proteins / Q000187:drug effects / Q000378:metabolism; D015415:Biomarkers; D002448:Cell Adhesion / Q000187:drug effects; D006801:Humans; D016297:Mutagenesis, Site-Directed / Q000502:physiology; D009414:Nerve Growth Factors / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D009432:Neural Crest / Q000166:cytology*; D009447:Neuroblastoma; D018696:Neuroprotective Agents / Q000378:metabolism / Q000494:pharmacology; D020917:Receptor, trkA / Q000187:drug effects / Q000378:metabolism; D017475:Receptors, Nerve Growth Factor / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D014407:Tumor Cells, Cultured / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D009353:Zinostatin / Q000494:pharmacology
D000970:Antineoplastic Agents; D001426:Bacterial Proteins; D015415:Biomarkers; D009414:Nerve Growth Factors; D018696:Neuroprotective Agents; D017475:Receptors, Nerve Growth Factor; D009353:Zinostatin; D020917:Receptor, trkA