Cloning and expression of the cDNA encoding rat tissue inhibitor of metalloproteinase 3 (TIMP-3).
We have cloned the cDNA encoding the rat tissue inhibitor of metalloproteinase 3 (TIMP-3) by a PCR cloning method. Sequence analysis reveals an open reading frame containing 211 amino acids that show 99% identity to mouse TIMP-3 and 95% identity to human TIMP-3, respectively. High-level expression of TIMP-3 was detected in rat kidney, lungs and heart.
Wu
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I
Laboratory for Surgical Research, Children's Hospital, Boston, MA 02115, USA.
Moses
M A
MA
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002813:Chondrosarcoma / Q000378:metabolism; D003001:Cloning, Molecular; D017124:Conserved Sequence; D017931:DNA Primers; D015870:Gene Expression; D006801:Humans; D007668:Kidney / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D008969:Molecular Sequence Data; D009206:Myocardium / Q000378:metabolism; D016366:Open Reading Frames; D009928:Organ Specificity; D016133:Polymerase Chain Reaction; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D019716:Tissue Inhibitor of Metalloproteinase-2
D017931:DNA Primers; D011506:Proteins; D012333:RNA, Messenger; D019716:Tissue Inhibitor of Metalloproteinase-2