Prediction of the percutaneous penetration and metabolism of dodecyl decaethoxylate in rats using in vitro models.
Percutaneous absorption of a lipophilic surfactant, dodecyl decaethoxylate, can be predicted using in vitro models. In vivo, dermal penetration of dodecyl decaethoxylate was found to be 22.9% in 48 h. All of the absorbed dodecyl decaethoxylate in the rat was metabolised and excreted in expired air as carbon dioxide, or in the urine and faeces. Using rat skin mounted in the unoccluded flow-through diffusion cell with MEM as receptor fluid, in vivo absorption was predicted by the percentage of the applied dose recovered in the stratum corneum, epidermis, dermis and receptor fluid at 24 h (25%). Conversely, the penetration of dodecyl decaethoxylate was over-predicted in the unoccluded static diffusion cell using aqueous ethanol (50% v/v) as the receptor fluid where 49.4% recovered in the receptor fluid at 24 h. In vitro models may be used to predict percutaneous absorption and reduce animal use, provided a suitable receptor fluid is used in which the penetrant is soluble. Dermal metabolism of dodecyl decaethoxylate was low and not considered to influence dermal absorption.
Roper
C S
CS
Department of Environmental and Occupational Medicine, University of Newcastle upon Tyne, UK.
Howes
D
D
Blain
P G
PG
Williams
F M
FM
D000287:Administration, Topical; D000818:Animals; D004058:Diffusion; D007851:Dodecanol / Q000031:analogs & derivatives* / Q000378:metabolism; D008297:Male; D008954:Models, Biological; D051381:Rats; D017208:Rats, Wistar; D012869:Skin Absorption / Q000187:drug effects*; D013501:Surface-Active Agents / Q000008:administration & dosage / Q000378:metabolism* / Q000493:pharmacokinetics
D013501:Surface-Active Agents; C097884:dodecyl decaethoxylate; D007851:Dodecanol