Analysis of cis-acting elements in the 5' leader sequence of alfalfa mosaic virus RNA 3.
The leader sequence of RNA 3 of the Leiden isolate of alfalfa mosaic virus strain 425 consists of 345 nucleotides (nt) and contains four putative stem-loop structures each with a motif in the loop that resembles the internal control region 2 (ICR2) of tRNA genes. The sequence of the 5' terminal 112 nt of this leader contains one of these stem-loop structures and is sufficient for a reduced accumulation of RNA 3 in protoplasts and a delayed accumulation in plants (E. A. G. van der Vossen et al., Nucleic Acids Res. 21, 1361-1367 (1993). A number of mutations were made in this 112-nt leader sequence to investigate its role in RNA 3 accumulation. Deletion of nucleotides 23-43, 44-90, or 55-112 and inversion or duplication of nucleotides 44-90 all abolished RNA 3 accumulation. Similarly, two base substitutions in the ICR2 motif (nucleotides 60-77) abolished RNA'3 accumulation. Mutations in the stem sections of the putative stem-loop structure had various effects on RNA 3 accumulation and supported the notion that this structure is important for plus-strand promoter activity.
van der Vossen
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EA
Institute of Molecular Plant Sciences, Gorlaeus Laboratories, Leiden University, The Netherlands.
Bol
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JF
D017794:Alfalfa mosaic virus / Q000235:genetics*; D001483:Base Sequence; D004279:DNA, Viral; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012333:RNA, Messenger / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D012367:RNA, Viral / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D012045:Regulatory Sequences, Nucleic Acid
D004279:DNA, Viral; D012333:RNA, Messenger; D012367:RNA, Viral