Infection and cellular activation by human T-cell leukemia viruses, types I and II.
Resting peripheral blood mononuclear cells (PBMC) or purified T-cells can be induced to proliferate when cocultured in vitro with fixed HTLV-infected T-cells. This process of HTLV-dependent cellular activation and induction of proliferation has been considered distinctive because of an apparent independence from conventional T-cell costimulatory signals. We have examined several HTLV-infected cell lines and found that proliferation was readily induced in resting PBMC by T-cells that were productively-infected with HTLV. However, equivalent HTLV-productive infection in a B-cell line failed to induce proliferation in PBMC, suggesting that HTLV-dependent induction of proliferation in PBMC was, at least in part, dependent upon a T-cell-specific signal. Furthermore, the induction of proliferation in PBMC populations was found to overlap with, and actually require, transfer and establishment of HTLV infection within the T-cell compartment of the PBMC population. These findings suggest that virus-induced activation of target cells may be directly associated with transfer and spread of HTLV infection.
Martin
T C
TC
Department of Microbiology, University of Minnesota, Minneapolis 55455, USA.
Southern
P J
PJ
D015703:Antigens, CD / Q000378:metabolism; D000998:Antiviral Agents / Q000494:pharmacology; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology*; D002455:Cell Division; D002460:Cell Line; D015686:Gene Products, env / Q000378:metabolism; D015368:Human T-lymphotropic virus 1 / Q000187:drug effects / Q000276:immunology* / Q000472:pathogenicity; D015367:Human T-lymphotropic virus 2 / Q000187:drug effects / Q000276:immunology* / Q000472:pathogenicity; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000276:immunology*; D008213:Lymphocyte Activation; D015682:Retroviridae Proteins, Oncogenic / Q000378:metabolism; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*; D014407:Tumor Cells, Cultured; D016047:Zalcitabine / Q000494:pharmacology; D015215:Zidovudine / Q000494:pharmacology; D054299:env Gene Products, Human Immunodeficiency Virus
D015703:Antigens, CD; D000998:Antiviral Agents; D015686:Gene Products, env; D015682:Retroviridae Proteins, Oncogenic; D054299:env Gene Products, Human Immunodeficiency Virus; C055266:gp46 protein, Human T-cell leukemia virus type I; C069568:gp46 protein, Human immunodeficiency virus 2; D015215:Zidovudine; D016047:Zalcitabine