[Increase in the effectiveness of site-specific cleavage of target DNA by tetranucleotide bleomycin derivatives using oligonucleotide-effectors].
The efficiency of site-specific interaction of target DNA with bleomycin derivatives of short nucleotides can be significantly increased using flanking effector oligonucleotides. The cleavage of a 20-mer single-stranded target DNA by a tetranucleotide containing a bleomycin A5 residue at the 5'-end was studied in the presence of effector oligonucleotides bearing phenazine residues at the 5'- and 3'-ends. In the presence of two effectors, the extent of the target DNA modification at 37 degrees C increased from 20 to 70%. Site-specific cleavage occurs, by up to 90%, at a single site of the target DNA. The melting temperature of the complementary complex formed by the bleomycin A5-modified tetranucleotide and target DNA in the presence of two effectors was 45 degrees C, whereas in the absence of the effectors, below 7 degrees C.
Vorob'ev
P E
PE
Markushin
Iu Ia
IuIa
Sergeev
D S
DS
Zarytova
V F
VF
D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D001483:Base Sequence; D001761:Bleomycin / Q000031:analogs & derivatives* / Q000494:pharmacology; D004247:DNA / Q000187:drug effects* / Q000378:metabolism; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000494:pharmacology*
D000903:Antibiotics, Antineoplastic; D009841:Oligonucleotides; D001761:Bleomycin; C025703:bleomycetin; D004247:DNA
Povyshenie éffektivnosti saĭt-spetsificheskogo rasshepleniia DNK- misheni bleomitsinovym proizvodnym tetranukleotida s pomoshch'iu oligonukleotidov-éffektorov.