Evaluation of the thymidine kinase (tk) locus as an insertion site in the highly attenuated vaccinia MVA strain.
The highly attenuated 'modified vaccinia Ankara' (MVA) strain is a potential live vaccine vector. Insertional inactivation of the tk-gene resulted in viruses difficult to purify. Co-integration of a functional fowlpox virus tk-gene allowed easy generation of recombinants, indicating that the genetically stable tk-gene region is a suitable insertion site, if tk-gene activity is substituted.
Scheiflinger
F
F
Biomedical Research Center, Immuno AG, Orth/Donau, Austria.
Falkner
F G
FG
Dorner
F
F
D000818:Animals; D002642:Chick Embryo; D016254:Mutagenesis, Insertional; D010957:Plasmids; D013937:Thymidine Kinase / Q000235:genetics*; D014613:Vaccines, Attenuated / Q000235:genetics*; D014614:Vaccines, Synthetic / Q000235:genetics; D014616:Vaccinia virus / Q000201:enzymology / Q000235:genetics*
D014613:Vaccines, Attenuated; D014614:Vaccines, Synthetic; D013937:Thymidine Kinase