title: Sequence, genomic structure, and chromosomal assignment of human DOC-2.
abstract: DOC-2 is a human gene originally identified as a 767-bp cDNA fragment isolated from normal ovarian epithelial cells by differential display against ovarian carcinoma cells. We have now determined the complete cDNA sequence of the 3.2-kb DOC-2 transcript and localized the gene to chromosome 5. A 12.5-kb genomic fragment at the 5'-end of DOC-2 has also been sequenced, revealing the intron-exon structure of the first eight exons (788 bases) of the DOC-2 gene. Translation of the DOC-2 cDNA predicts a hydrophobic protein of 770 amino acid residues with a molecular weight of 82.5 kDa. Comparison of the DNA and amino acid sequences of DOC-2 to publicly accessible sequence databases revealed 83% identify to p96, a murine protein of similar size, thought to be a mitogen-responsive phosphoprotein. In addition, about 45% identity was observed between the first 140 N-terminal residues of DOC-2 and the Caenorhabditas elegans M110.5 and Drosophila melanogaster Dab genes.
authors.affiliation: Eccles Institute of Human Genetics, Salt Lake City, Utah, USA. Hans.Albertsen@genetics.utah.edu
authors.forename: H M
authors.initials: HM
authors.lastname: Albertsen
authors.forename: S A
authors.initials: SA
authors.lastname: Smith
authors.forename: R
authors.initials: R
authors.lastname: Melis
authors.forename: B
authors.initials: B
authors.lastname: Williams
authors.forename: P
authors.initials: P
authors.lastname: Holik
authors.forename: J
authors.initials: J
authors.lastname: Stevens
authors.forename: R
authors.initials: R
authors.lastname: White
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D033942:Adaptor Proteins, Vesicular Transport; D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D051017:Apoptosis Regulatory Proteins; D001483:Base Sequence; D002460:Cell Line; D002895:Chromosomes, Human, Pair 5; D018076:DNA, Complementary; D005091:Exons; D005260:Female; D016147:Genes, Tumor Suppressor; D006801:Humans; D007438:Introns; D008969:Molecular Sequence Data; D011506:Proteins / Q000235:genetics*; D012377:Rodentia; D017386:Sequence Homology, Amino Acid; D025521:Tumor Suppressor Proteins
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D033942:Adaptor Proteins, Vesicular Transport; D051017:Apoptosis Regulatory Proteins; C102885:DAB2 protein, human; D018076:DNA, Complementary; D011506:Proteins; D025521:Tumor Suppressor Proteins