title: Differentiation of follicular dendritic cells and full antibody responses require tumor necrosis factor receptor-1 signaling.
abstract: Using mice double deficient for tumor necrosis factor (TNF) and lymphotoxin alpha (LT alpha), we demonstrated that TNF and/or LT alpha are necessary for development of a normal splenic microarchitecture and for isotype switch after immunization with sheep red blood cells (SRBC). In the present study, we extended these observations by determining which TNF receptor (TNFR) is involved in morphological and functional differentiation of the spleen. Spleen morphology and antibody response were investigated in wild-type, TNFR1-/-, TNFR2-/- and TNF/LT alpha-/- mice immunized with SRBC. TNF/LT alpha-/- mice, which have a complete disruption of the TNF/LT alpha signaling system including the LT beta-receptor pathway, displayed an abnormal microarchitecture, and isotype switch did not take place. TNFR1-/- and TNFR2-/- mice displayed a normal spleen microarchitecture and mounted an IgM and IgG antibody response to SRBC. However, the IgG production in TNFR1-/- mice was minimal, with citers leveling off 6 d after immunization. In this strain, immunofluorescence revealed a lack of follicular dendritic cells (FDC) network, detected with FDC-M1 as well as anti-CR1, and a lack of germinal centers, detected with peanut agglutinin. In conclusion, whereas normal splenic microarchitecture and isotype switch might require the LT beta receptor, differentiation of FDC network, development of germinal centers, and full IgG response depend on signaling via TNFR1.
authors.affiliation: Swiss Institute of Technology, Institute of Toxicology, Schwerzenbach, Switzerland.
authors.forename: M
authors.initials: M
authors.lastname: Le Hir
authors.forename: H
authors.initials: H
authors.lastname: Bluethmann
authors.forename: M H
authors.initials: MH
authors.lastname: Kosco-Vilbois
authors.forename: M
authors.initials: M
authors.lastname: Müller
authors.forename: F
authors.initials: F
authors.lastname: di Padova
authors.forename: M
authors.initials: M
authors.lastname: Moore
authors.forename: B
authors.initials: B
authors.lastname: Ryffel
authors.forename: H P
authors.initials: HP
authors.lastname: Eugster
mesh_terms: D000818:Animals; D000917:Antibody Formation; D002454:Cell Differentiation; D003433:Crosses, Genetic; D003713:Dendritic Cells / Q000166:cytology* / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D017578:Immunoglobulin Class Switching; D007074:Immunoglobulin G / Q000097:blood / Q000145:classification; D007075:Immunoglobulin M / Q000097:blood; D008233:Lymphotoxin-alpha / Q000235:genetics* / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D008817:Mice, Mutant Strains; D018124:Receptors, Tumor Necrosis Factor / Q000502:physiology*; D015398:Signal Transduction; D013045:Species Specificity; D013154:Spleen / Q000166:cytology / Q000276:immunology
chemical_list: D007074:Immunoglobulin G; D007075:Immunoglobulin M; D008233:Lymphotoxin-alpha; D018124:Receptors, Tumor Necrosis Factor