title: Induction of immediate-early, ornithine decarboxylase and antizyme gene expression in the rat small intestine after transient ischaemia.
abstract: The expression of the immediate early genes (IEG)s c-fos, c-jun and zif/268, and the genes coding for ornithine decarboxylase (ODC) and its regulatory protein antizyme (AZ), was studied in rat small intestine following transient ischemia. The ischemic stimulus for 10 min alone did not alter the expression of these genes. A rapid and transitory induction of all IEG mRNAs occurred in a coordinated manner peaking at 30 min following recirculation and returned to basal levels 3 hr after recirculation. Protein products of the IEGs accumulated in the smooth muscle layer of the intestine by 2-3 hr after recirculation. Expression of both ODC and AZ mRNAs initially decreased to 70% of control levels 1 hr after recirculation but markedly increased at 2 to 4 hr after recirculation. The functional significance of these changes in gene expression in relation to tissue integrity and function after the ischaemia/reperfusion is discussed.
authors.affiliation: Department of Biochemistry, University of Queensland, St Lucia, Australia.
authors.forename: Z
authors.initials: Z
authors.lastname: Pujic
authors.forename: I
authors.initials: I
authors.lastname: Matsumoto
authors.forename: A
authors.initials: A
authors.lastname: Yamataka
authors.forename: T
authors.initials: T
authors.lastname: Miyano
authors.forename: P
authors.initials: P
authors.lastname: Wilce
mesh_terms: D000818:Animals; D004268:DNA-Binding Proteins / Q000235:genetics; D051766:Early Growth Response Protein 1; D015971:Gene Expression Regulation, Enzymologic; D017781:Genes, Immediate-Early; D016762:Genes, fos; D016758:Genes, jun; D017874:Immediate-Early Proteins; D007421:Intestine, Small / Q000098:blood supply / Q000201:enzymology*; D007511:Ischemia / Q000201:enzymology / Q000235:genetics*; D008297:Male; D009955:Ornithine Decarboxylase / Q000235:genetics*; D011506:Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D014157:Transcription Factors / Q000235:genetics
chemical_list: D004268:DNA-Binding Proteins; D051766:Early Growth Response Protein 1; C486110:Egr1 protein, rat; D017874:Immediate-Early Proteins; D011506:Proteins; D012333:RNA, Messenger; D014157:Transcription Factors; C031017:ornithine decarboxylase antizyme; D009955:Ornithine Decarboxylase