Comparative fluorescence in situ hybridization mapping of primate chromosomes with Alu polymerase chain reaction generated probes from human/rodent somatic cell hybrids.
We have used Alu polymerase chain reaction generated probes from rearranged human/rodent somatic cell hybrids for fluorescence in situ hybridization and comparative mapping of some intrachromosomal changes in the karyotypes of great apes (Pan troglodytes, P. paniscus, Gorilla gorilla, Pongo pygmaeus), a gibbon (Hylobates lar), and an Old World monkey (Macaca fuscata). Probes containing chromosomes 2 and 18 fragments confirmed inversions already suggested by the banding pattern of great ape homologues. However, a chromosome 3 fragment showed complex rearrangements in the gibbon and macaque karyotype which were previously not well defined from banding. 'Subchromosomal painting' will allow the identification of intrachromosomal changes on the basis of DNA homology and provides a powerful method to study karyological and genomic evolution.
Müller
S
S
Institut für Anthropologie und Humangenetik, Universität München, Germany.
Koehler
U
U
Weinberg
J
J
Marzella
R
R
Finelli
P
P
Antonacci
R
R
Rocchi
M
M
Archidiacono
N
N
D000818:Animals; D005075:Biological Evolution; D002871:Chromosome Banding; D002874:Chromosome Mapping / Q000379:methods*; D002887:Chromosomes, Human, Pair 18 / Q000737:chemistry; D002889:Chromosomes, Human, Pair 2 / Q000737:chemistry; D015342:DNA Probes; D015321:Gene Rearrangement; D015186:Hominidae / Q000235:genetics*; D006801:Humans; D006822:Hybrid Cells / Q000737:chemistry*; D006922:Hylobates / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D008251:Macaca / Q000235:genetics*; D051379:Mice; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D051381:Rats; D012091:Repetitive Sequences, Nucleic Acid
D015342:DNA Probes