Structural heterogeneity of phospholipase D in 10 dicots.
The occurrence of multiple forms of phospholipase D (EC 3.1.4.4) was investigated in different tissues of castor bean (Ricinus communis) and in other plant species. Phospholipase D variants were resolved by nondenaturing and isoelectric focusing polyacrylamide gel electrophoresis and detected by immunoblotting using anti-phospholipase D antibodies and by enzyme activity assay. Three phospholipase D variants were produced differentially in the roots, endosperm, cotyledons, and hypocotyl of 5-day postgermination seedlings of castor bean. Furthermore, different patterns of phospholipase D variants were found in the different regions of hypocotyl (elongated and hook). Multiple phospholipase D forms were found in florets of cauliflower and broccoli, leaves of cabbage, celery, tomato, and potato, and alfalfa sprouts, suggesting that structural heterogeneity of phospholipase D occurs widely in plants.
Dyer
J H
JH
Department of Biochemistry, Kansas State University, Manhattan, 66506, USA.
Zheng
S
S
Wang
X
X
D004591:Electrophoresis, Polyacrylamide Gel; D007887:Fabaceae / Q000201:enzymology*; D007525:Isoelectric Focusing; D010739:Phospholipase D / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D010946:Plants, Medicinal; D013045:Species Specificity
D010739:Phospholipase D