Construction of a mouse whole-genome radiation hybrid panel and application to MMU11.
Whole-genome radiation hybrids have been used to construct human genome maps that integrate different types of markers. To investigate this methodology in mammalian species other than humans, a panel of 164 mouse x hamster whole-genome radiation hybrids was constructed. This set of hybrids was used to produce a high-resolution map of a region on MMU11 that included microsatellite markers and cDNA sequences. The mouse homologue of the human SRY-related gene SOX9 was mapped to an interval of approximately 1.1 cM flanked by the microsatellite markers D11Mit11 and D11Mit291. This interval includes the region containing the mouse Tail-short mutation, a possible homologue of the human syndrome campomelic dysplasia, which is caused by mutations in SOX9. Our results suggest that whole-genome radiation hybrid technology will be a useful adjunct to mapping the genomes of nonhuman mammalian species.
Schmitt
K
K
Department of Genetics, University of Cambridge, Downing Street, Cambridge, CB2 3EH, United Kingdom.
Foster
J W
JW
Feakes
R W
RW
Knights
C
C
Davis
M E
ME
Spillett
D J
DJ
Goodfellow
P N
PN
D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D006224:Cricetinae; D017931:DNA Primers; D004276:DNA, Satellite; D005819:Genetic Markers; D016678:Genome; D006609:High Mobility Group Proteins / Q000235:genetics; D006801:Humans; D006822:Hybrid Cells / Q000528:radiation effects; D008297:Male; D051379:Mice / Q000235:genetics*; D008817:Mice, Mutant Strains; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D055758:SOX9 Transcription Factor; D012733:Sex Differentiation; D013234:Stem Cells / Q000528:radiation effects; D014157:Transcription Factors / Q000235:genetics
D017931:DNA Primers; D004276:DNA, Satellite; D005819:Genetic Markers; D006609:High Mobility Group Proteins; D055758:SOX9 Transcription Factor; C527932:SOX9 protein, human; C527933:Sox9 protein, mouse; D014157:Transcription Factors