Patch-clamp detection of neurotransmitters in capillary electrophoresis.
Gamma-aminobutyrate acid, L-glutamate, and N-methyl-D-aspartate were separated by capillary electrophoresis and detected by the use of whole-cell and outside-out patch-clamp techniques on freshly dissociated rat olfactory interneurons. These neuroactive compounds could be identified from their electrophoretic migration times, unitary channel conductances, and power spectra that yielded corner frequencies and mean single-channel conductances characteristic for each of the different agonist-receptor interactions. This technique has the sensitivity to observe the opening of a single ion channel for agonists separated by capillary electrophoresis.
Orwar
O
O
Department of Chemistry, Stanford University, California 94305, USA.
Jardemark
K
K
Jacobson
I
I
Moscho
A
A
Fishman
H A
HA
Scheller
R H
RH
Zare
R N
RN
D000818:Animals; D015374:Biosensing Techniques; D019075:Electrophoresis, Capillary; D018698:Glutamic Acid / Q000032:analysis* / Q000302:isolation & purification; D007395:Interneurons / Q000737:chemistry*; D007473:Ion Channels / Q000502:physiology; D016202:N-Methylaspartate / Q000032:analysis* / Q000302:isolation & purification; D009830:Olfactory Bulb / Q000166:cytology; D018408:Patch-Clamp Techniques; D051381:Rats; D018079:Receptors, GABA / Q000502:physiology; D017470:Receptors, Glutamate / Q000502:physiology; D016194:Receptors, N-Methyl-D-Aspartate / Q000502:physiology; D012680:Sensitivity and Specificity; D005680:gamma-Aminobutyric Acid / Q000032:analysis* / Q000302:isolation & purification
D007473:Ion Channels; D018079:Receptors, GABA; D017470:Receptors, Glutamate; D016194:Receptors, N-Methyl-D-Aspartate; D018698:Glutamic Acid; D005680:gamma-Aminobutyric Acid; D016202:N-Methylaspartate