A cDNA encoding canine muscle-type phosphofructokinase.
The canine muscle-type-phosphofructokinase-encoding gene (M-PFK) was sequenced by using a combination of cDNA cloning and RT-PCR amplification. The canine M-PFK sequence shares 88 and 90% identity with rabbit and human M-PFK, respectively. The canine ORF was determined to be 6-bp longer than either human or rabbit M-PFK due to a 6-bp insertion at the end of exon 13.
Smith
B F
BF
School of Veterinary Medicine, University of Pennsylvania, Philadelphia 19104-6010, USA. smithbf@mail.auburn.edu
Henthorn
P S
PS
Rajpurohit
Y
Y
Stedman
H
H
Wolfe
J H
JH
Patterson
D F
DF
Giger
U
U
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004251:DNA Transposable Elements; D018076:DNA, Complementary; D004285:Dogs / Q000235:genetics*; D015723:Gene Library; D006801:Humans; D007527:Isoenzymes / Q000235:genetics*; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000201:enzymology*; D010732:Phosphofructokinase-1 / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011817:Rabbits; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
D004251:DNA Transposable Elements; D018076:DNA, Complementary; D007527:Isoenzymes; D010732:Phosphofructokinase-1