Expressed sequence tags from the long arm of human chromosome 21.
We isolated expressed sequence tags (ESTs) on the long arm of chromosome 21. The ESTs were mapped by PCR using a monochromosomal somatic-cell mapping panel. Of a total of 55 cDNAs, 30 mapped back uniquely to chromosome 21, 7 mapped back to other chromosomes including chromosome 21, 8 mapped back to chromosomes other than 21, and 10 could not be assigned using this methodology. The 30 chromosome 21-specific markers so isolated represent useful EST markers. A rapid PCR-based method was used to delineate the expression pattern of these 30 pairs in different tissues.
Chiang
P W
PW
Howard Hughes Medical Institute, University of Michigan Medical Center, MSRBI, Ann Arbor 48109-0650, USA.
Dzida
G
G
Grumet
J
J
Cheng
J F
JF
Song
W J
WJ
Crombez
E
E
Van Keuren
M L
ML
Kurnit
D M
DM
D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D002891:Chromosomes, Human, Pair 21; D017931:DNA Primers; D018076:DNA, Complementary; D005819:Genetic Markers; D006801:Humans; D006822:Hybrid Cells; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D016324:Sequence Tagged Sites
D017931:DNA Primers; D018076:DNA, Complementary; D005819:Genetic Markers