title: A single amino acid change in acetolactate synthase confers resistance to valine in tobacco.
abstract: The metabolic control of branches chain amino acid (BCAA) biosynthesis involves allosteric regulation of acetolactate synthase (ALS) by the end-products of the pathway, valine, leucine and isoleucine. We describe here the molecular basis of valine resistance. We cloned and sequenced an ALS gene from the tobacco mutant Valr-1 and found a single basepair substitution relative to the wild-type allele. This mutation causes a serine to leucine change in the amino acid sequence of ALS at position 214. We then mutagenized the wild-type allele of the ALS gene of Arabidopsis and found that it confers valine resistance when introduced into tobacco plants. Taken together, these results suggest that the serine to leucine change at position 214 of ALS is responsible for valine resistance in tobacco.
authors.affiliation: Laboratoire de Biologie Cellulaire, INRA, Versailles, France.
authors.forename: F
authors.initials: F
authors.lastname: Hervieu
authors.forename: H
authors.initials: H
authors.lastname: Vaucheret
mesh_terms: D000095:Acetolactate Synthase / Q000235:genetics*; D000595:Amino Acid Sequence; D017360:Arabidopsis; D001482:Base Composition; D001483:Base Sequence; D001665:Binding Sites; D018744:DNA, Plant; D004351:Drug Resistance / Q000235:genetics; D015870:Gene Expression; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D010947:Plants, Toxic; D013329:Structure-Activity Relationship; D014026:Nicotiana / Q000187:drug effects / Q000235:genetics; D014633:Valine / Q000494:pharmacology*
chemical_list: D018744:DNA, Plant; D000095:Acetolactate Synthase; D014633:Valine