title: Outcome of intracytoplasmic sperm injection with testicular spermatozoa in obstructive and non-obstructive azoospermia.
abstract: From 1 August 1993 until 30 September 1994, 69 couples suffering from azoospermia underwent testicular sperm extraction and intracytoplasmic sperm injection. In 50 couples with obstructive azoospermia a total of 631 metaphase-II oocytes were injected after testicular sperm extraction yielding a 2-PN fertilization rate of 57%. In female patients <40 years of age an ongoing pregnancy rate per transfer of 42% (14/33) was obtained. So far, eight healthy babies have been born, including two singletons and three twin gestations. In 19 couples with non-obstructive azoospermia a total of 264 metaphase-II oocytes were injected after testicular sperm extraction, yielding a 2-PN fertilization rate of 58%. An ongoing pregnancy rate per transfer of 31% (5/16) was established. So far, six healthy babies have been born including one singleton, one twin and one triplet gestation.
authors.affiliation: Centre for Reproductive Medicine, University Hospital, Dutch-speaking Brussels Free University, Laarbeeklaan 101, 1090 Brussels, Belgium.
authors.forename: P
authors.initials: P
authors.lastname: Devroey
authors.forename: P
authors.initials: P
authors.lastname: Nagy
authors.forename: H
authors.initials: H
authors.lastname: Tournaye
authors.forename: J
authors.initials: J
authors.lastname: Liu
authors.forename: S
authors.initials: S
authors.lastname: Silber
authors.forename: A
authors.initials: A
authors.lastname: Van Steirteghem
mesh_terms: D000328:Adult; D003593:Cytoplasm; D004624:Embryo Transfer; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D006801:Humans; D008297:Male; D008845:Microinjections; D008875:Middle Aged; D009845:Oligospermia / Q000628:therapy*; D011247:Pregnancy; D011256:Pregnancy Outcome; D013094:Spermatozoa; D013737:Testis / Q000166:cytology*