title: Molecular cloning of human hyaluronan synthase.
abstract: We report here the cDNA sequence of human hyaluronan synthase. The cDNA clone was isolated from a human fetal brain cDNA library by screening with a DNA probe generated from the coding sequence of murine hyaluronan synthase. It contains a 2107 bp cDNA insert which is composed of a 1629 bp open reading frame and a short segment of the 3'-untranslated region. Comparative analysis of the predicted coding region with the murine hyaluronan synthase sequence indicates 84.4% and 96.0% identity in nucleotide sequence and amino acid sequences, respectively. Northern analysis indicated that the gene was ubiquitously expressed in various human tissues.
authors.affiliation: Institute for Molecular Science of Medicine, Aichi Medical University, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Itano
authors.forename: K
authors.initials: K
authors.lastname: Kimata
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers / Q000737:chemistry; D018076:DNA, Complementary / Q000235:genetics; D015870:Gene Expression; D014453:Glucuronosyltransferase / Q000235:genetics*; D016695:Glycosyltransferases; D006801:Humans; D000076002:Hyaluronan Synthases; D008565:Membrane Proteins; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014166:Transferases; D029867:Xenopus Proteins
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D008565:Membrane Proteins; D012333:RNA, Messenger; D029867:Xenopus Proteins; D014166:Transferases; D016695:Glycosyltransferases; D014453:Glucuronosyltransferase; C056944:HAS1 protein, Xenopus; D000076002:Hyaluronan Synthases