A new pathway for protein export in Saccharomyces cerevisiae.
Several physiologically important proteins lack a classical secretory signal sequence, yet they are secreted from cells. To investigate the secretion mechanism of such proteins, a representative mammalian protein that is exported by a nonclassical mechanism, galectin-1, has been expressed in yeast. Galectin-1 is exported across the yeast plasma membrane, and this export does not require the classical secretory pathway nor the yeast multidrug resistance-like protein Ste6p, the transporter for the peptide a factor. A screen for components of the export machinery has identified genes that are involved in nonclassical export. These findings demonstrate a new pathway for protein export that is distinct from the classical secretory pathway in yeast.
Cleves
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AE
Department of Biochemistry and Biophysics, University of California, San Francisco, 94143-0534, USA. cleves@cgl.ucsf.edu
Cooper
D N
DN
Barondes
S H
SH
Kelly
R B
RB
D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001693:Biological Transport, Active / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics; D000939:Epitopes / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D037483:Galectin 1; D005800:Genes, Fungal; D006023:Glycoproteins; D006388:Hemagglutinins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins
D018528:ATP-Binding Cassette Transporters; D018076:DNA, Complementary; D004271:DNA, Fungal; D000939:Epitopes; D005656:Fungal Proteins; D037483:Galectin 1; D006023:Glycoproteins; D006388:Hemagglutinins; D011994:Recombinant Proteins; C489277:STE6 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins