title: Studies on intranuclear inclusions and nuclear grooves in papillary thyroid cancer by light, scanning electron and transmission electron microscopy.
abstract: OBJECTIVE
  To successively examine intranuclear inclusions and nuclear grooves in the same papillary thyroid cancer specimens using a light microscope (LM), scanning electron microscope (SEM) and transmission electron microscope (TEM).
  
  
  METHODS
  We stained cells by the Papanicolaou method after fixation in 1.25% glutaraldehyde for LM and then attempted to observe them successively by SEM-TEM after fixation in 2% paraformaldehyde and 2% osmium tetroxide.
  
  
  RESULTS
  On SEM, intranuclear inclusions were observed as elevated parts, like hills, and nuclear grooves were observed as deep fissures or shallow cracks, sometimes with a few in one cell. On TEM, both intranuclear inclusions and nuclear grooves seemed formed by the nuclear membranes. Intranuclear inclusions also possessed cytoplasm and/or cytoplasmic organelles within some expanded areas in the nuclear grooves.
  
  
  CONCLUSIONS
  It was evident from our three-step technique that intranuclear inclusions and nuclear grooves were essentially the same structures.
authors.affiliation: Institute for Comprehensive Medical Science, Fujita Health University School of Medicine, Toyoake, Aichi, Japan.
authors.forename: C
authors.initials: C
authors.lastname: Kaneko
authors.forename: M
authors.initials: M
authors.lastname: Shamoto
authors.forename: H
authors.initials: H
authors.lastname: Niimi
authors.forename: A
authors.initials: A
authors.lastname: Osada
authors.forename: M
authors.initials: M
authors.lastname: Shimizu
authors.forename: M
authors.initials: M
authors.lastname: Shinzato
mesh_terms: D001707:Biopsy, Needle; D002291:Carcinoma, Papillary / Q000473:pathology* / Q000648:ultrastructure; D002467:Cell Nucleus / Q000473:pathology* / Q000648:ultrastructure; D006801:Humans; D002479:Inclusion Bodies / Q000473:pathology* / Q000648:ultrastructure; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D013194:Staining and Labeling; D013964:Thyroid Neoplasms / Q000473:pathology* / Q000648:ultrastructure