Effect of crude fractions of psoralea corylifolia seed extract on bone calcification.
Non-polar crude fractions (Ho-0 and Ho-1) of an acetone extract of Psoralea corylifolia seeds were administrated orally to untreated and experimental rachitic rats. In the biological screening of the fractions, Ho-1, an elution with n-hexane-ethyl acetate by column chromatography over silica gel of the acetone extract, untreated rats showed a significant elevation of the serum inorganic phosphorus and revealed histomorphometrically a significant increase in bone calcification. When Ho-0, an elution with n-hexane, and Ho-1 were administrated to the rachitic rats fed with a vitamin D-free, low-phosphorus diet, they not only increased significantly the concentration of inorganic phosphorus in serum, but also significantly promoted bone calcification. Administration of 30 mg/kg of Ho-1 resulted in a marked decrease of osteoid volume and improvement of hyperosteoidosis in rachitic rats. These results suggested that Ho-0 and Ho-1 are useful as a remedy for bone fracture, osteomalacia, osteoporosis, and related conditions.
Miura
H
H
Hikone Research Laboratories, Maruho Co., Ltd., Shiga, Japan.
Nishida
H
H
Linuma
M
M
D000818:Animals; D002113:Calcification, Physiologic / Q000187:drug effects*; D002118:Calcium / Q000097:blood; D007887:Fabaceae; D008297:Male; D010710:Phosphates / Q000097:blood; D010936:Plant Extracts / Q000494:pharmacology*; D010946:Plants, Medicinal; D051381:Rats; D011916:Rats, Inbred F344; D017208:Rats, Wistar; D012279:Rickets / Q000097:blood / Q000188:drug therapy* / Q000503:physiopathology; D012639:Seeds
D010710:Phosphates; D010936:Plant Extracts; D002118:Calcium