title: Growth of cultured human bronchiogenic epithelioid CCD-14 Br cells and dermal fibroblasts, NB1 RGB treated with ginseng tetrapeptide and its isomer.
abstract: The configurations of the component amino acids in ginseng tetrapeptide 1 isolated from Panax ginseng were determined by HPLC with an optical resolution column and the structure of 1 was established to be H-L-Val-gamma-D-Glu-D-Arg- Gly-OH. Synthesis of the ginseng tetrapeptide, 1, and of the configuration and conjugation isomers, H-L-Val-gamma-L-Glu-L-Arg-Gly-OH (2), H-L-Val-D-Glu- D-Arg-Gly-OH (3), and H-L-Val-L-Glu-L-Arg-Gly-OH (4) was carried out by a solid-phase method using the Fmoc strategy. The effects of 1-4 on the proliferation of baby hamster kidney (BHK)-21 cells, normal female bronchiogenic epithelioid (CCD-14 Br) cells, and normal human epidermal fibroblast (NB1 RGB) were examined. Only 1 showed 32 and 23% enhancement of BHK-21 and human CCD-14 Br cells growth, respectively, at a concentration of 13.6 microM and 41% enhancement of NB1 RGB cells growth at a concentration of 32 microM under the conditions employed. It was shown that both the configuration of the component amino acids and the peptide conjugation at a gamma-position of D-Glu in 1 are important for proliferation of the cells. Compound 1 exerted a prominent effect on cell stimulation and growth rate without any morphological change and showed no cytotoxicity.
authors.affiliation: Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama University, Hiroshima, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Yagi
authors.forename: T
authors.initials: T
authors.lastname: Ishizu
authors.forename: N
authors.initials: N
authors.lastname: Okamura
authors.forename: S
authors.initials: S
authors.lastname: Noguchi
authors.forename: H
authors.initials: H
authors.lastname: Itoh
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001980:Bronchi; D002455:Cell Division / Q000187:drug effects*; D002460:Cell Line; D006224:Cricetinae; D004848:Epithelium; D005260:Female; D005347:Fibroblasts; D006801:Humans; D007536:Isomerism; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000138:chemical synthesis / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D005894:Panax; D010946:Plants, Medicinal; D012867:Skin
chemical_list: D009842:Oligopeptides; C099793:valyl-glutamyl-arginyl-glycine