Interferon induction by HIV-1-infected cells: a possible role of sulfatides or related glycolipids.
We have investigated the mechanism of interferon (IFN) induction in peripheral blood mononuclear cells by HIV-1(IIIB)-infected H9 cells or by recombinant gp120. A monoclonal antibody specific for the galactosylsphingosinyl moiety in galactocerebrosides and sulfatides inhibited IFN induction in a dose-dependent manner. Furthermore, exogenous sulfatides inhibited with an ID50 of approximately 1 microM, whereas galactocerebrosides were not inhibitory at 40 times higher concentrations. These studies suggest that sulfate containing galactolipids such as sulfatides on responder cells may be part of the gp120-membrane complex that initiates the induction of IFN. A partial homology of an epitope on the V3 loop of gp 120 with a previously suggested binding domain for sulfated glycoconjugates supports this conclusion.
Ankel
H
H
Medical College of Wisconsin, Milwaukee 53226, USA.
Capobianchi
M R
MR
Frezza
F
F
Castilletti
C
C
Dianzani
F
F
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000276:immunology; D002478:Cells, Cultured; D006017:Glycolipids / Q000276:immunology*; D015699:HIV Envelope Protein gp120 / Q000276:immunology; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D016898:Interferon-alpha / Q000096:biosynthesis*; D008969:Molecular Sequence Data; D010455:Peptides / Q000276:immunology; D011609:Psychosine / Q000276:immunology; D013433:Sulfoglycosphingolipids / Q000276:immunology*; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D006017:Glycolipids; D015699:HIV Envelope Protein gp120; D016898:Interferon-alpha; D010455:Peptides; D013433:Sulfoglycosphingolipids; D011609:Psychosine