Antibodies specific for the DNA quadruplex [d(CGC G4 GCG)4] isolated from autoimmune mice.
An autoantibody specific for a DNA quadruplex structure has been isolated and cloned from three-month-old autoimmune "viable motheaten" mice. This antibody (mev-alpha Q1) has been tested extensively in vitro and found to bind specifically and preferentially to the parallel-stranded quadruplex structure formed by the oligonucleotide d(CGC G4 GCG). The anti-quadruplex antibody does not show specific affinity for single-, double-, or triple-stranded oligonuclotides of similar CG-rich sequence motifs.
Brown
B A
BA
Department of Biochemistry, North Carolina State University, Raleigh 27695, USA.
Lin
Y
Y
Roberts
J F
JF
Hardin
C C
CC
D000818:Animals; D000974:Antibodies, Antinuclear / Q000235:genetics / Q000302:isolation & purification*; D000918:Antibody Specificity; D015551:Autoimmunity / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000737:chemistry* / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D008969:Molecular Sequence Data
D000974:Antibodies, Antinuclear; D004247:DNA