title: Regulation of neutral cholesterol esterase activity by phospholipids containing negative charges in substrate liposome.
abstract: The effect of phospholipids on cholesteryl ester hydrolysis by neutral cholesterol esterase in alveolar macrophages was studied. Among the phospholipids used as emulsifiers, those with a negative charge, such as phosphatidylserine, phosphatidic acid, phosphatidylinositol, and cardiolipin, gave a higher level of hydrolysis by neutral cholesterol esterase than other less negatively charged phospholipids, such as phosphatidylcholine, lysophosphatidylcholine, phosphatidylethanolamine, and sphingomyelin. Phospholipase D treatment of liposomes emulsified with phosphatidylcholine produced phosphatidic acid and enhanced cholesteryl ester hydrolysis. Phospholipase A2 treatment produced lysophosphatidylcholine and decreased the hydrolysis. The hydrolysis of cholesteryl ester in lipid droplets obtained from cholesterol-laden macrophages elicited by thioglycollate in the rat peritoneal cavity was low compared to artificial liposomes emulsified with phosphatidylcholine. The reason for this was speculated to be that lipid droplets were low in total phospholipids and poor in phospholipids with strong negative charges but rich in phosphatidylethanolamine and sphingomyelin. These results suggest that the polar heads of phospholipids may play an important role in cholesteryl ester hydrolysis by neutral cholesterolesterase.
authors.affiliation: Department of Biochemistry, Faculty of Pharmaceutical Sciences, Chiba University, Japan.
authors.forename: I
authors.initials: I
authors.lastname: Ishii
authors.forename: R
authors.initials: R
authors.lastname: Onozaki
authors.forename: E
authors.initials: E
authors.lastname: Takahashi
authors.forename: S
authors.initials: S
authors.lastname: Takahashi
authors.forename: N
authors.initials: N
authors.lastname: Fujio
authors.forename: T
authors.initials: T
authors.lastname: Harada
authors.forename: N
authors.initials: N
authors.lastname: Morisaki
authors.forename: K
authors.initials: K
authors.lastname: Shirai
authors.forename: Y
authors.initials: Y
authors.lastname: Saito
authors.forename: S
authors.initials: S
authors.lastname: Hirose
mesh_terms: D000818:Animals; D002788:Cholesterol Esters / Q000378:metabolism; D004563:Electrochemistry; D006868:Hydrolysis; D008079:Lipoproteins, VLDL / Q000032:analysis; D008081:Liposomes; D016676:Macrophages, Alveolar / Q000201:enzymology*; D010740:Phospholipases / Q000378:metabolism; D010743:Phospholipids / Q000737:chemistry / Q000502:physiology*; D011817:Rabbits; D051381:Rats; D017208:Rats, Wistar; D002787:Sterol Esterase / Q000187:drug effects / Q000378:metabolism*; D013379:Substrate Specificity; D013656:Taurocholic Acid / Q000494:pharmacology
chemical_list: D002788:Cholesterol Esters; D008079:Lipoproteins, VLDL; D008081:Liposomes; D010743:Phospholipids; D013656:Taurocholic Acid; D010740:Phospholipases; D002787:Sterol Esterase