title: [The use of the polymerase chain reaction in the forensic medical study of hairs].
abstract: In order to detect polymorphic DNA sequences in the bulbs of single human hairs, polymerase chain reaction (PCR) was used. Specific hypervariable regions of ApoB gene, D1S80 locus, and sex-specific sequences of X/Y chromosomes were amplified. The amount of DNA isolated from single hair bulbs was measured by fluorometry. Methodological aspects of using PCR for forensic medical examinations of single hairs are discussed.
authors.forename: G F
authors.initials: GF
authors.lastname: Puchkov
authors.forename: Iu V
authors.initials: IuV
authors.lastname: Kukhar'kov
authors.forename: V V
authors.initials: VV
authors.lastname: Korban
authors.forename: S R
authors.initials: SR
authors.lastname: Borovko
mesh_terms: D004247:DNA / Q000032:analysis / Q000302:isolation & purification; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005554:Forensic Medicine / Q000379:methods*; D006197:Hair / Q000737:chemistry*; D018859:Hair Follicle / Q000737:chemistry; D006257:Head; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods*; D012727:Sex Characteristics
chemical_list: D004247:DNA
vernacular_title: Primenenie polimeraznoĭ tsepnoĭ reaktsii pri sudebno-meditsinskom issledovanii volos.