Estimating minimum allele frequencies for DNA profile frequency estimates for PCR-based loci.
In order that there can be confidence that DNA profile frequency estimates will not place undue bias against a defendant, 2 methods are described for estimating minimum allele frequency bounds for PCR-based loci. One approach estimates minimum allele frequencies for VNTR and STR loci using sample size and the observed heterozygosity at a locus, while the second approach, appropriate for loci typed with allele-specific oligonucleotide probes, is based only on sample size. The use of a minimum allele frequency enables compensation for sparse sampling of infrequent alleles in population databases.
Budowle
B
B
Forensic Science Research and Training Center, FBI Laboratory, FBI Academy, Quantico, Virginia 22135, USA.
Monson
K L
KL
Chakraborty
R
R
D016172:DNA Fingerprinting; D005787:Gene Frequency; D006579:Heterozygote; D006801:Humans; D018598:Minisatellite Repeats; D008957:Models, Genetic; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction; D018401:Sample Size
D015345:Oligonucleotide Probes