Sequence-dependent in vivo importation of tRNAs into the mitochondrion of Leishmania tarentolae.
Sequence determinants for the importation of tRNAs into the mitochondrion of Leishmania tarentolae in vivo were investigated. tRNA(Ile)(UAU) is exclusively localized within the mitochondrion and tRNA(Gln)(CUG) exclusively in the cytosol (Lye LF, Chen DHT, Suyama Y, 1993, Mol Biochem Parasitol 58:233-246; Shi X, Chen DHT, Suyama Y, 1994, Mol Biochem Parasitol 65:23-37). L. tarentolae cells were transfected with plasmids encoding either tRNA(Ile) or tRNA (Gln) that were tagged with altered sequences in the D loop, permitting discrimination from the endogenous tRNAs. Primer extension analysis was used to show that the plasmid-encoded genes were expressed and that the tagged tRNAs showed a similar intracellular localization as the endogenous tRNAs. Exchange or deletion of the 5'-flanking genomic sequences had no effect on the expression or mitochondrial localization of the tagged tRNA(Ile) or on the expression or cytosolic localization of the tagged tRNA(Gln), suggesting that the signals for importation are localized within the tRNA itself. Swapping the D loop+stem from the exclusively cytosolic tRNA(Gln) with that from the tRNA(Ile) produced a partial mitochondrial localization of the plasmid-expressed mutated tRNA(Gln). However, D loop exchange did not eliminate the mitochondrial localization of the plasmid-expressed mutated tRNA(Ile), suggesting that tertiary structure or additional sequence elements may be involved in the importation signal.
Lima
B D
BD
Departamento de Biologia Celular, Universidade de Brasilia, Brazil.
Simpson
L
L
D000818:Animals; D001483:Base Sequence; D003600:Cytosol / Q000378:metabolism; D017931:DNA Primers; D007891:Leishmania / Q000235:genetics / Q000378:metabolism*; D008928:Mitochondria / Q000378:metabolism*; D008969:Molecular Sequence Data; D010957:Plasmids; D016053:RNA, Protozoan / Q000378:metabolism*; D012351:RNA, Transfer, Gln / Q000378:metabolism*; D012355:RNA, Transfer, Ile / Q000378:metabolism*; D014162:Transfection; D014170:Transformation, Genetic
D017931:DNA Primers; D016053:RNA, Protozoan; D012351:RNA, Transfer, Gln; D012355:RNA, Transfer, Ile