Purification and identification of allergenic alpha (2u)-globulin species of rat urine.
Amino-acid compositional and sequence analyses as well as mass spectrometric determinations of purified rat urine proteins, previously termed prealbumin and alpha(2)-euglobulin, have revealed a high homology between the two forms which have now been identified as alpha(2)-globulin species. The "prealbumin' fraction was found to correspond to alpha(2u)-globulin originating from salivary gland and the 'alpha(2)-euglobulin' fraction was identical with the major urinary protein (MUP) or alpha(2u)-globulin. The results indicate that the two major protein fractions of rat urine constitute different forms of the same parent protein, alpha(2u)-globulin, having no amino-acid sequence resemblance to prealbumin (transthyretin) of rat serum.
Bayard
C
C
Division of Respiratory Allergy and Immunology, National Institute for Working Life, Solna, Sweden.
Holmquist
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L
Vesterberg
O
O
D000485:Allergens / Q000737:chemistry / Q000302:isolation & purification* / Q000652:urine; D000510:Alpha-Globulins / Q000737:chemistry / Q000276:immunology / Q000652:urine*; D000595:Amino Acid Sequence; D000818:Animals; D008297:Male; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011506:Proteins / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D051381:Rats; D017207:Rats, Sprague-Dawley
D000485:Allergens; D000510:Alpha-Globulins; D011506:Proteins; C013803:alpha 2u globulin; C035737:major urinary proteins