title: Analysis of the catalytic domain of the lysin of the lactococcal bacteriophage Tuc2009 by chimeric gene assembling.
abstract: An active chimeric cell wall lytic enzyme (Tsl) has been constructed by fusing the region coding for the N-terminal half of the lactococcal phage Tuc2009 lysin and the region coding for the C-terminal domain of the major pneumococcal autolysin. The chimeric enzyme exhibited a glycosidase activity capable of hydrolysing choline-containing pneumococcal cell walls. This experimental approach demonstrated that the Tuc2009 lysin possesses a modular structure and further supports the hypothesis that many cell wall lytic enzymes have evolved by the fusion of preexisting catalytic and peptidoglycan-binding domains.
authors.affiliation: Departamento de Microbiología Molecular, Centro de Investigaciones Biológicas, Consejo Superior de Investigaciones Científicas, Madrid, Spain.
authors.forename: M M
authors.initials: MM
authors.lastname: Sheehan
authors.forename: J L
authors.initials: JL
authors.lastname: García
authors.forename: R
authors.initials: R
authors.lastname: López
authors.forename: P
authors.initials: P
authors.lastname: García
mesh_terms: D000581:Amidohydrolases / Q000235:genetics / Q000378:metabolism; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics; D001435:Bacteriophages / Q000737:chemistry* / Q000201:enzymology / Q000235:genetics*; D001483:Base Sequence; D004591:Electrophoresis, Polyacrylamide Gel; D006026:Glycoside Hydrolases / Q000235:genetics / Q000378:metabolism; D017045:Lactococcus / Q000737:chemistry* / Q000201:enzymology / Q000821:virology; D008969:Molecular Sequence Data; D010957:Plasmids; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics; D013296:Streptococcus pneumoniae / Q000737:chemistry / Q000201:enzymology; D015678:Viral Structural Proteins / Q000737:chemistry / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D011993:Recombinant Fusion Proteins; C088289:S protein, bacteriophage Tuc2009; D015678:Viral Structural Proteins; D006026:Glycoside Hydrolases; D000581:Amidohydrolases; C019665:amidase