title: The superiority of hollow fiber membrane over bubble oxygenator in a perfusion circuit for the evaluation of small caliber endothelialized arterial prostheses.
abstract: A perfusion circuit was constructed from a pneumatic ventricular assist device, 2 compliance chambers, 4 small-diameter silicone tubes (ID 4 mm) simulating shear inducing vascular prostheses, and an oxygenator with a heat exchanger. A bubble oxygenator (in a BO circuit) and a hollow fiber membrane oxygenator (in an MO circuit) were studied. The circuits were perfused with 30% human serum containing culture medium for 7 days at 37 degrees C. The pH, Po2, PCo2, Na+, K+, Ca2+, Cl, glucose, and total protein concentrations remained the same in BO and MO circuits during the 7 days of perfusion. The differences between the values measured in the perfusion medium and in the medium maintained in the static conditions of cell culture were not significant. In the BO circuit, the amount of cholesterol and triglyceride concentrations decreased whereas the relative amounts of albumin, alpha 1, alpha 2, beta, and gamma globulins remained stable in the perfusion medium. The medium from the BO circuit did not promote the proliferation of cultured human saphenous vein endothelial cells. In the medium from the MO circuit, the cholesterol and triglyceride concentrations did not change with perfusion time; the proliferation rate and anticoagulant function of endothelial cells were maintained. The hollow fiber membrane oxygenator preserves the biological characteristics of the cell culture medium in a perfusion circuit. The MO circuit permits the performance of relevant studies on shear stress resistance and functional activity of human endothelial cells seeded onto vascular prostheses.
authors.affiliation: Centre de Recherches Chirurgicales Henri Mondor, C.N.R.S., Hôpital Henri Mondor, Créteil, France.
authors.forename: J P
authors.initials: JP
authors.lastname: Mazzucotelli
authors.forename: L
authors.initials: L
authors.lastname: Lecouls
authors.forename: A
authors.initials: A
authors.lastname: Hamzaoui
authors.forename: C
authors.initials: C
authors.lastname: Philippon
authors.forename: E
authors.initials: E
authors.lastname: Bizouard
authors.forename: M
authors.initials: M
authors.lastname: Moczar
authors.forename: D Y
authors.initials: DY
authors.lastname: Loisance
mesh_terms: D001807:Blood Vessel Prosthesis / Q000592:standards*; D002118:Calcium / Q000378:metabolism; D002452:Cell Count; D002478:Cells, Cultured; D002712:Chlorides / Q000378:metabolism; D002784:Cholesterol / Q000378:metabolism; D001026:Coronary Artery Bypass; D004730:Endothelium, Vascular / Q000166:cytology* / Q000502:physiology; D006353:Heart-Assist Devices; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008567:Membranes, Artificial; D010101:Oxygen Consumption / Q000502:physiology; D010477:Perfusion; D011188:Potassium / Q000378:metabolism; D012501:Saphenous Vein / Q000166:cytology / Q000378:metabolism; D012964:Sodium / Q000378:metabolism; D014280:Triglycerides / Q000378:metabolism
chemical_list: D002712:Chlorides; D008567:Membranes, Artificial; D014280:Triglycerides; D002784:Cholesterol; D012964:Sodium; D011188:Potassium; D002118:Calcium