Distribution of myristoyl-CoA:protein N-myristoyl transferase activity in rabbit intestine.
Myristoyl-CoA:protein N-myristoyl transferase (NMT) attaches the fatty acid, myristate, to the amino-terminal glycine residue of various proteins involved in cellular regulation and/or signal transduction. We report differences in the activity and properties of NMT in New Zealand rabbit small intestine, ascending colon and descending colon. The mucosa of the small intestine, ascending colon and descending colon was assayed for NMT activity using peptides of known myristoylated proteins (pp60src and catalytic subunit of cAMP dependent protein kinase). Total NMT activity per gram tissue was 5-fold higher in the small intestine and 1.5-fold higher in the ascending colon than in the descending colon. Smooth muscle from the colon also contained low levels of NMT activity. NMT activity was 2- to 3-fold higher in the particulate fraction than in the cytosolic fraction of the mucosa in the descending colon. The apparent molecular mass of NMT in the intestine mucosa was 78 kDa.
Magnuson
B A
BA
Department of Pathology, College of Medicine, University of Saskatchewan, Saskatoon, Canada.
Raju
R V
RV
Sharma
R K
RK
D000214:Acyl Coenzyme A / Q000378:metabolism; D000217:Acyltransferases / Q000737:chemistry / Q000378:metabolism*; D000818:Animals; D002458:Cell Fractionation; D002850:Chromatography, Gel; D003106:Colon / Q000201:enzymology*; D007082:Ileum / Q000201:enzymology*; D007413:Intestinal Mucosa / Q000201:enzymology*; D007700:Kinetics; D008297:Male; D008970:Molecular Weight; D009130:Muscle, Smooth / Q000201:enzymology; D010446:Peptide Fragments / Q000378:metabolism; D011817:Rabbits
D000214:Acyl Coenzyme A; D010446:Peptide Fragments; C040349:S-tetradecanoyl-coenzyme A; D000217:Acyltransferases; C050480:glycylpeptide N-tetradecanoyltransferase