title: The linkage map of sheep Chromosome 6 compared with orthologous regions in other species.
abstract: The genetic linkage map of sheep Chromosome (Chr) 6 has been extended to include 35 loci with the addition of 11 RFLP and 12 microsatellite loci. The sex-averaged linkage map now spans 154 cM from phosphodiesterase cyclic GMP beta polypeptide (PDE6B) to OarCP125, an anonymous sheep microsatellite. The male and female map lengths, at 180 cM and 132 cM respectively, did not differ significantly. The physical assignment of PDE6B to Chr 6q33-qter orientates the linkage map on sheep Chr 6 with PDE6B near the telomere and OarCP125 towards the centromere. The order and genetic distances between loci are similar for the sheep Chr 6 and cattle Chr 6 maps, except for the position of the casein genes. The sheep Chr 6 linkage map is also comparable to portions of human Chr 4, mouse Chrs 5 and 3, and pig Chr 8. The synteny between sheep Chr 6 and human Chr 4 has been extended from PDE6B (4p16.3) to epidermal growth factor (EGF, 4q25-q27). However, a region from platelet-derived growth factor receptor alpha polypeptide (PDGFRA) to bone morphogenetic protein 3 (BMP3), which spans 19 cM on sheep Chr 6, appears to be inverted with respect to the human and mouse loci. Other differences in the gene order between sheep, pig, and mouse suggest more complex rearrangements.
authors.affiliation: AgResearch Molecular Biology Unit, Department of Biochemistry and Centre for Gene Research, University of Otago, P.O. Box 56, Dunedin, New Zealand.
authors.forename: E A
authors.initials: EA
authors.lastname: Lord
authors.forename: J M
authors.initials: JM
authors.lastname: Lumsden
authors.forename: K G
authors.initials: KG
authors.lastname: Dodds
authors.forename: H M
authors.initials: HM
authors.lastname: Henry
authors.forename: A M
authors.initials: AM
authors.lastname: Crawford
authors.forename: H A
authors.initials: HA
authors.lastname: Ansari
authors.forename: P D
authors.initials: PD
authors.lastname: Pearce
authors.forename: D W
authors.initials: DW
authors.lastname: Maher
authors.forename: R T
authors.initials: RT
authors.lastname: Stone
authors.forename: S M
authors.initials: SM
authors.lastname: Kappes
authors.forename: C W
authors.initials: CW
authors.lastname: Beattie
authors.forename: G W
authors.initials: GW
authors.lastname: Montgomery
mesh_terms: D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping / Q000662:veterinary*; D018076:DNA, Complementary / Q000235:genetics; D005260:Female; D005298:Fertility / Q000235:genetics; D008040:Genetic Linkage; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D012756:Sheep / Q000235:genetics*; D013552:Swine / Q000235:genetics
chemical_list: D018076:DNA, Complementary