title: Fine structural cytochemical and immunocytochemical analysis of nucleic acids and ribonucleoprotein distribution in nuclei of pig oocytes and early preimplantation embryos.
abstract: The fine structure of pig oocytes at the germinal vesicle (GV) stage and early preimplantation embryos (one to four blastomeres) isolated at slaughter was investigated by cytochemical and immunocytochemical methods. The distribution of nucleic acids and ribonucleoproteins (RNPs) in "compact nucleoli" [denominated nucleolus-like bodies (NLB) in oocytes and nucleolus precursor bodies (NPB) in early embryos] and in intranuclear bodies or granules was investigated by staining methods preferential for nuclear RNPs or using the osmium ammine or ethidium bromide-phosphotungstic acid (EB-PTA) reactions for nucleic acids. The distributions of the Sm antigen of nucleoplasmic small nuclear RNPs (snRNPs), the methyl-3 guanosine (m3G) cap of snRNAs and the splicing factor SC-35 were detected by immunoelectron microscopy using specific antibodies. The RNP nature of both NLBs and NPBs, and of nuclear granules in oocytes and embryos, and of fibrillar strands radially projecting from NLBs was revealed. Cytochemical evidence for RNA as a component of NLBs was further provided by EB-PTA staining in combination with the enzymatic removal of RNA, or by osmium-ammine staining without previous acid hydrolysis, while the absence of DNA in NLBs was established by Feulgen-like osmium-ammine staining. In addition, autoradiography demonstrated the absence of [6-3H]thymidine incorporation into NPBs. Other autoradiographic evidence attested the accumulation of RNA in NLBs of oocytes after a 60 min in vitro pulse of [5-3H]uridine. Immunoelectron microscopy using specific antibodies revealed the occurrence of nucleoplasmic snRNPs in both NLBs and NPBs. The presence of snRNA in NLB was confirmed by means of an antibody recognizing the m3G-cap structure. Another spliceosomal component, the protein SC-35 was also detected in NLBs. Among the numerous and variable intranuclear granules occurring mostly in aggregates, the Sm antigen was clearly detected only in the interchromatin granule-type component. Some Sm labeling was occasionally seen in other categories of larger granules. No reaction was detected over any granules when using the anti-m3G-cap antibody. The aggregates consisting of large granules and a finely fibrillar component were intensely immunolabeled by the anti-SC-35 splicing factor probe. Our observations suggest that the compact nucleoli, known to be present before and after fertilization in mammals (NLBs of oocytes and NPBs of early embryos), represent nuclear structural elements containing nonnucleolar, spliceosomal components.
authors.affiliation: Centre of Electron Microscopy, University of Lausanne, Bugnon 27, CH-1005 Lausanne, Switzerland.
authors.forename: V
authors.initials: V
authors.lastname: Kopecny
authors.forename: M
authors.initials: M
authors.lastname: Biggiogera
authors.forename: J
authors.initials: J
authors.lastname: Laurincik
authors.forename: J
authors.initials: J
authors.lastname: Pivko
authors.forename: P
authors.initials: P
authors.lastname: Grafenau
authors.forename: T E
authors.initials: TE
authors.lastname: Martin
authors.forename: X D
authors.initials: XD
authors.lastname: Fu
authors.forename: S
authors.initials: S
authors.lastname: Fakan
mesh_terms: D000818:Animals; D000906:Antibodies; D001345:Autoradiography / Q000379:methods; D002466:Cell Nucleolus / Q000502:physiology / Q000648:ultrastructure; D004622:Embryo, Mammalian / Q000737:chemistry / Q000648:ultrastructure*; D047108:Embryonic Development / Q000502:physiology*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008854:Microscopy, Electron / Q000379:methods; D009687:Nuclear Proteins / Q000737:chemistry / Q000276:immunology; D009696:Nucleic Acids / Q000737:chemistry*; D009865:Oocytes / Q000737:chemistry* / Q000502:physiology; D011247:Pregnancy; D012313:RNA / Q000032:analysis / Q000737:chemistry; D012261:Ribonucleoproteins / Q000737:chemistry* / Q000235:genetics; D017411:Ribonucleoproteins, Small Nuclear / Q000737:chemistry / Q000276:immunology; D000068103:Serine-Arginine Splicing Factors; D013194:Staining and Labeling; D013552:Swine
chemical_list: D000906:Antibodies; D009687:Nuclear Proteins; D009696:Nucleic Acids; D012261:Ribonucleoproteins; D017411:Ribonucleoproteins, Small Nuclear; C073288:SRSF2 protein, human; D000068103:Serine-Arginine Splicing Factors; D012313:RNA