Solid-phase extraction for the selective isolation of polycyclic aromatic hydrocarbons, azaarenes and heterocyclic aromatic amines in charcoal-grilled meat.
A method for the simultaneous analysis of 12 mutagenic and/or carcinogenic compounds is described; these substances belong to three different chemical groups: polycyclic aromatic hydrocarbons (PAHs), azaarenes, i.e., nitrogen-containing polycyclic aromatic hydrocarbons (PANHs), and heterocyclic aromatic amines (HAAs). The selective enrichment procedure includes coupling of solid-phase extraction (SPE) steps using diatomaceous earth, propylsulfonic acid, silica gel and octadecylsilane columns. The eluted fractions were analysed by high-performance liquid chromatography with UV and electrochemical detection. Levels measured were estimated to be 4-19 ng g-1. Peak confirmation was carried out by GC-MS for both PAHs and PANHs, and by LC with a photodiode array detector for HAAs. The method was applied to the analysis of charcoal-grilled meat and was judged to be generally applicable for detection of these mutagens at the ppb level in processed foods.
Rivera
L
L
Departament de Química Analítica, Universitat de Barcelona, Spain.
Curto
M J
MJ
Pais
P
P
Galceran
M T
MT
Puignou
L
L
D000588:Amines / Q000032:analysis / Q000302:isolation & purification; D001372:Aza Compounds / Q000032:analysis / Q000302:isolation & purification*; D002273:Carcinogens / Q000032:analysis / Q000302:isolation & purification; D002606:Charcoal; D002851:Chromatography, High Pressure Liquid; D003296:Cooking; D008401:Gas Chromatography-Mass Spectrometry; D006571:Heterocyclic Compounds / Q000032:analysis / Q000302:isolation & purification*; D008460:Meat / Q000032:analysis*; D009153:Mutagens / Q000032:analysis / Q000302:isolation & purification; D011083:Polycyclic Compounds / Q000032:analysis / Q000302:isolation & purification*; D013056:Spectrophotometry, Ultraviolet
D000588:Amines; D001372:Aza Compounds; D002273:Carcinogens; D006571:Heterocyclic Compounds; D009153:Mutagens; D011083:Polycyclic Compounds; D002606:Charcoal