The polyether bistratene A activates protein kinase C-delta and induces growth arrest in HL60 cells.
Bistratene A (BisA) induced growth arrest in G2/M in HL60 cells. In addition, BisA-treated cells (50 nM for 48 h) became adherent and expressed the adhesion molecule CD11c, but did not express the monocyte enzyme alpha-napthyl acetate esterase or phagocytose complement coated yeasts. BisA activated protein kinase C (PKC)-delta and induced translocation of PKC-delta to the nucleus. This suggests that activation of PKC-delta can induce growth arrest and cell adhesion, but is insufficient to mediate full differentiation of HL60 cells. BisA has potential as a new probe for determining the function of PKC isoenzymes, specifically PKC-delta.
Griffiths
G
G
Department of Immunology, Birmingham University, United Kingdom.
Garrone
B
B
Deacon
E
E
Owen
P
P
Pongracz
J
J
Mead
G
G
Bradwell
A
A
Watters
D
D
Lord
J
J
D000081:Acetamides; D002448:Cell Adhesion / Q000187:drug effects; D002451:Cell Compartmentation / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D004789:Enzyme Activation / Q000187:drug effects; D004988:Ethers, Cyclic / Q000494:pharmacology*; D019084:Fluorescent Antibody Technique, Indirect; D006131:Growth Inhibitors / Q000494:pharmacology*; D018922:HL-60 Cells; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism*; D010766:Phosphorylation; D011493:Protein Kinase C / Q000378:metabolism*; D051745:Protein Kinase C-delta; D011714:Pyrans; D013141:Spiro Compounds
D000081:Acetamides; D004988:Ethers, Cyclic; D006131:Growth Inhibitors; D007527:Isoenzymes; D011714:Pyrans; D013141:Spiro Compounds; C059975:bistratene A; C497258:PRKCD protein, human; D011493:Protein Kinase C; D051745:Protein Kinase C-delta