Modifications of tumor-associated antigen expression on human lung cancer cells by hyperthermia and cytokine.
The effect of hyperthermia and cytokine (IFN-gamma) in the expression of carcinoembryonic antigen (CEA) on the surface of cells was studied in vitro. The human lung cancer cell line, GLL-1, was used. The results demonstrate increase in CEA expression after hyperthermia. The magnitude of elevated CEA expression increased with increasing temperature (41-43 degrees C). Time-course study demonstrated that the peak CEA expression was 3 days after heating at 43 degrees C. The relative CEA expression was 1.3 at 1 day, 1.6 at 2 days, 1.9 at 3 days, 1.8 at 4 days, and 1.5 at 5 days, respectively. Hyperthermia plus IFN-gamma showed a synergistic effect in the expression of CEA. The four fold marked increase of CEA expression was detected. Furthermore, the pattern of time-course of CEA expression in hyperthermia combined with IFN-gamma was different from that in hyperthermia alone.
Takahashi
T
T
Department of Radiology and Radiation Oncology, Gunma University School of Medicine, Japan.
Mitsuhashi
N
N
Sakurai
H
H
Niibe
H
H
D000230:Adenocarcinoma / Q000473:pathology*; D000368:Aged; D000951:Antigens, Neoplasm / Q000096:biosynthesis* / Q000235:genetics; D002272:Carcinoembryonic Antigen / Q000096:biosynthesis* / Q000235:genetics; D002453:Cell Cycle; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D006801:Humans; D006979:Hyperthermia, Induced; D007371:Interferon-gamma / Q000494:pharmacology*; D008175:Lung Neoplasms / Q000473:pathology*; D008297:Male; D011994:Recombinant Proteins; D014407:Tumor Cells, Cultured
D000951:Antigens, Neoplasm; D002272:Carcinoembryonic Antigen; D011994:Recombinant Proteins; D007371:Interferon-gamma