title: Urine trypsinogen-2 as marker of acute pancreatitis.
abstract: We examined the clinical utility of urine trypsinogen-2 as a marker of acute pancreatitis (AP). Fifty-nine patients with AP, 42 with acute abdominal diseases of extrapancreatic origin, and 63 without evidence of acute abdominal disease were studied. Urine trypsinogen-2 was determined by a time-resolved immunofluorometric assay. As reference methods we used serum trypsinogen-2, urine amylase, and serum amylase. The diagnostic accuracy of the markers was evaluated by receiver-operating characteristic (ROC) analysis. At admission, urine trypsinogen-2 differentiated patients with AP from controls with high accuracy. The area under the ROC curve (AUC) was 0.978, which was equal to that of serum trypsinogen-2 (0.998) and serum amylase (0.969) and significantly larger than that of urine amylase. For differentiation between severe and mild AP, urine trypsinogen-2 (0.730) was equal to serum trypsinogen-2 (0.721), and clearly better than amylase in serum and urine. These results suggest that determination of urine trypsinogen-2 is a useful test to detect AP and to evaluate disease severity.
authors.affiliation: Department of Clinical Chemistry, University of Helsinki, Finland.
authors.forename: J
authors.initials: J
authors.lastname: Hedström
authors.forename: V
authors.initials: V
authors.lastname: Sainio
authors.forename: E
authors.initials: E
authors.lastname: Kemppainen
authors.forename: P
authors.initials: P
authors.lastname: Puolakkainen
authors.forename: R
authors.initials: R
authors.lastname: Haapiainen
authors.forename: E
authors.initials: E
authors.lastname: Kivilaakso
authors.forename: K O
authors.initials: KO
authors.lastname: Schauman
authors.forename: U H
authors.initials: UH
authors.lastname: Stenman
mesh_terms: D000208:Acute Disease; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000681:Amylases / Q000097:blood / Q000652:urine; D005260:Female; D015200:Fluoroimmunoassay; D006801:Humans; D008297:Male; D008875:Middle Aged; D010195:Pancreatitis / Q000175:diagnosis / Q000652:urine*; D012016:Reference Values; D014357:Trypsin; D014362:Trypsinogen / Q000097:blood / Q000652:urine*
chemical_list: C064831:PRSS2 protein, human; D014362:Trypsinogen; D000681:Amylases; D014357:Trypsin