The interaction of macrophage and non-macrophage tropic isolates of HIV-1 with thymic and tonsillar dendritic cells in vitro.
Dendritic cells isolated from thymus and tonsil were tested for susceptibility to HIV-1 strains that are tropic for macrophages or for T cell lines. DCs were purified by cell sorting and before infection expressed high levels of CD4 and HLA-DR and lacked markers for T, B, NK cells, or macrophages. Viral entry and reverse transcription was found after pulsing with strains of HIV-1 that could infect macrophages. During the first 36 h the PCR signals for gag sequences increased in DCs and macrophages. In contrast little if any viral DNA was found after pulsing macrophages or DCs with HIV-1 that was able to infect T cell lines. DCs pulsed with HIV-1 were able to transmit infection to responding T cells during an allogeneic or superantigen response. Selection for virus able to infect lymphoid DCs and other DCs expressing CD4 and its transfer to T cells during subsequent immune responses may provide a mechanism for the observed predominance of macrophage-tropic HIV-1 after in vivo transmission.
Cameron
P U
PU
AIDS Pathogenesis Unit, Macfarlane Burnet Centre for Medical Research, Fairfield Hospital, Victoria, Australia.
Lowe
M G
MG
Sotzik
F
F
Coughlan
A F
AF
Crowe
S M
SM
Shortman
K
K
D015704:CD4 Antigens / Q000032:analysis; D002469:Cell Separation; D002648:Child; D003713:Dendritic Cells / Q000821:virology*; D015750:Genes, gag; D054303:HIV Reverse Transcriptase; D015497:HIV-1 / Q000254:growth & development*; D006684:HLA-DR Antigens / Q000032:analysis; D006801:Humans; D008264:Macrophages / Q000821:virology; D014066:Palatine Tonsil / Q000166:cytology / Q000821:virology*; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D013601:T-Lymphocytes / Q000821:virology; D013950:Thymus Gland / Q000166:cytology / Q000821:virology*; D014158:Transcription, Genetic
D015704:CD4 Antigens; D006684:HLA-DR Antigens; D054303:HIV Reverse Transcriptase; D012194:RNA-Directed DNA Polymerase