A 11.5-kb 5'-terminal cDNA sequence of chicken breast muscle connectin/titin reveals its Z line binding region.
A partial 5'-region cDNA (11.5 kb) of chicken breast muscle connectin/titin encoding 3752 amino acids was sequenced. The predicted amino acid sequence contains 31 immunoglobulin C2 motifs and 10 interdomains. The sequence suggests a skeletal muscle type of connectin isoforms. Immunoelectron microscopic studies using antisera raised against several products of the cDNA fragments expressed in E. coli revealed that a region of some 800 amino acids from the N terminus of connectin is involved in its binding to the Z line in a sarcomere.
Yajima
H
H
Department of Biology, Faculty of Science, Chiba University, Japan.
Ohtsuka
H
H
Kawamura
Y
Y
Kume
H
H
Murayama
T
T
Abe
H
H
Kimura
S
S
Maruyama
K
K
D000818:Animals; D001665:Binding Sites; D002352:Carrier Proteins / Q000096:biosynthesis; D002645:Chickens; D003001:Cloning, Molecular; D064211:Connectin; D018076:DNA, Complementary / Q000737:chemistry / Q000378:metabolism*; D004926:Escherichia coli; D015723:Gene Library; D005982:Glutathione Transferase; D016253:Microscopy, Immunoelectron; D009124:Muscle Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000648:ultrastructure; D018482:Muscle, Skeletal / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism; D011494:Protein Kinases / Q000096:biosynthesis* / Q000235:genetics / Q000648:ultrastructure; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure; D012518:Sarcomeres / Q000378:metabolism / Q000648:ultrastructure
D002352:Carrier Proteins; D064211:Connectin; D018076:DNA, Complementary; D009124:Muscle Proteins; D011993:Recombinant Fusion Proteins; D005982:Glutathione Transferase; D011494:Protein Kinases