Regenerated cellulose-based hemodialyzers with immobilized proteins as potential devices for extracorporeal immunoadsorption procedures: an assessment of protein coupling capacity and in vitro dialysis performances.
The development of immunoadsorbents usable with whole blood should offer the potential for making significant improvements in extracorporeal immunoadsorption procedures. In contrast to traditional chromatographic media, these hemocompatible matrices could be used without requiring the previous step of the separation of blood cells and plasma. Conventional hemodialyzers seem to be particularly appropriate for such a purpose. This paper describes a feasibility study of the preparation of immunoaffinity supports, from regenerated cellulose (Cuprophan)-based dialyzers by cyanogen bromide activation and coupling of bovine serum albumin or human immunoglobin G, used as models for immunochemical ligands. Several parameters of the activation and coupling steps were studied in order to define the optimum preparation conditions. In addition, the preservation of the transport properties (clearance and ultrafiltration) of the modified hemodialyzers was evaluated in vitro to ensure that the device potentially could be used in future human therapeutic applications with no risk of massive removal of solutes and fluid from the blood. Results indicate that 150-300 mg of immunoglobulins can be immobilized per square meter of Cuprophan. Modified hemodialyzers show a slight decrease of their clearance values and a slight increase of their ultrafiltration coefficients, and thus they can be proposed as reliable carrier material for extracorporeal cleansing systems.
Vallar
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Centre de Recherche INSERM, C.H.U. de Brabois, Vandoeuvre-lés-Nancy, France.
Rivat
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C
D000818:Animals; D002417:Cattle; D002482:Cellulose / Q000031:analogs & derivatives / Q000737:chemistry / Q000378:metabolism*; D003124:Colorimetry; D003488:Cyanogen Bromide / Q000737:chemistry / Q000378:metabolism; D005112:Extracorporeal Circulation; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism; D007163:Immunosorbent Techniques; D008024:Ligands; D008567:Membranes, Artificial; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*; D006435:Renal Dialysis; D012710:Serum Albumin, Bovine / Q000378:metabolism; D014462:Ultrafiltration
D007074:Immunoglobulin G; D008024:Ligands; D008567:Membranes, Artificial; D011506:Proteins; D012710:Serum Albumin, Bovine; D002482:Cellulose; C009865:cuprammonium cellulose; D003488:Cyanogen Bromide