title: [Gangliosides with 8-O-methyl-N-glycolylneuraminic acid and N-acetylgalactosamine from the star fish Asterias rathbuni].
abstract: The structure of gangliosides isolated from the liver of starfish Asterias rathbuni was established by chemical methods, mass spectrometry, and enzymic hydrolysis with a neuraminidase. The major ganglioside was found to be a disialoganglioside with two 8-O-methyl-N-glycolylneuraminic acid residues bound with an N-acetylgalactosamine residue: 8-O-Me-NeuGc alpha 2-3 (8-O-Me-NeuGc alpha 2-6)GalNAc beta 1-3Gal beta 1-4Glc beta 1-1 Cer. Its lipid part contains unsubstituted higher fatty acids (mainly palmitic and stearic) and sphingosines (for the most part C20 and C22). The minor ganglioside fraction consists of two monosialogangliosides the carbohydrate chains of which differed only in the position of the terminal 8-O-methyl-N-glycolylneuraminic acid residue in the N-acetylgalactosamine moiety: 8-O-Me-NeuGc alpha 2-3GalNAc beta 1-3Gal beta 1-4Glc beta 1-1 Cer and 8-O-Me-NeuGc alpha 2-6GalNAc beta 1-3Gal beta 1-4Glc beta 1-1 Cer. The higher fatty acid composition of the minor gangliosides was determined too.
authors.forename: N V
authors.initials: NV
authors.lastname: Chekareva
authors.forename: G P
authors.initials: GP
authors.lastname: Smirnova
authors.forename: V L
authors.initials: VL
authors.lastname: Sadovskaia
mesh_terms: D000818:Animals; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002849:Chromatography, Gas; D005732:Gangliosides / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D008099:Liver / Q000737:chemistry; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009438:Neuraminic Acids / Q000737:chemistry*; D013215:Starfish / Q000737:chemistry*
chemical_list: C099765:8-O-methyl-N-glycolylneuraminic acid; D005732:Gangliosides; D009438:Neuraminic Acids
vernacular_title: Gangliozidy s 8-O-mitil-N-glikolilneĭraminovoĭ kislotoĭ i N-atsetilgalaktozaminom iz morskoĭ zvezdy Asterias rathbuni.