Molecular characterization of a ribonucleotide reductase (nrdF) gene fragment of Mycoplasma hyopneumoniae and assessment of the recombinant product as an experimental vaccine for enzootic pneumonia.
A Mycoplasma hyopneumoniae clone bank was screened with hyperimmune pig serum. One clone exhibited sequence homology to the prokaryotic R2 subunit of ribonucleotide reductase and was expressed as an 11-kDa protein fused to beta-galactosidase. The vaccine potential of the fusion protein was assessed in pig trials. Following experimental challenge with a virulent isolate of M. hyopneumoniae, gross lung pathology (mean Goodwin lung score) of vaccinated animals, irrespective of adjuvant treatment, was significantly reduced compared with that of control unvaccinated pigs (P < 0.05).
Fagan
P K
PK
Department of Biological Sciences, University of Wollongong, New South Wales, Australia.
Djordjevic
S P
SP
Eamens
G J
GJ
Chin
J
J
Walker
M J
MJ
D000595:Amino Acid Sequence; D000818:Animals; D001428:Bacterial Vaccines / Q000276:immunology*; D001483:Base Sequence; D008969:Molecular Sequence Data; D009174:Mycoplasma / Q000201:enzymology / Q000235:genetics / Q000276:immunology*; D018410:Pneumonia, Bacterial / Q000517:prevention & control / Q000662:veterinary*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D012264:Ribonucleotide Reductases / Q000235:genetics / Q000276:immunology*; D013552:Swine; D013553:Swine Diseases / Q000517:prevention & control*; D014614:Vaccines, Synthetic / Q000276:immunology
D001428:Bacterial Vaccines; D011993:Recombinant Fusion Proteins; D014614:Vaccines, Synthetic; D012264:Ribonucleotide Reductases