title: Molecular cloning and nucleotide sequences of cDNA clones of sheep and goat adrenocortical cytochromes P450scc (CYP11A1).
abstract: We isolated cDNA clones of the mRNAs for cytochromes P450scc (CYP11A1) from sheep and goat adrenocortices using the RT-PCR method. We determined the complete nucleotide sequences of the coding and 3'-flanking regions of these cDNA clones. The results confirmed the amino terminal sequence of the sheep cytochrome P450scc reported previously [Miyatake et al., Biochim. Biophys. Acta 1215,176-182 (1994)]. On the basis of comparison of the deduced amino acid sequences of various animals and rainbow trout cytochromes P450scc, and other mitochondrial cytochrome P450 isozymes, we discussed the substrate-associated and adreno-ferredoxin-binding region of cytochrome P450scc.
authors.affiliation: Department of Biochemistry, Kagawa Medical School, Japan.
authors.forename: E
authors.initials: E
authors.lastname: Okuyama
authors.forename: T
authors.initials: T
authors.lastname: Okazaki
authors.forename: A
authors.initials: A
authors.lastname: Furukawa
authors.forename: R F
authors.initials: RF
authors.lastname: Wu
authors.forename: Y
authors.initials: Y
authors.lastname: Ichikawa
mesh_terms: D000302:Adrenal Cortex / Q000201:enzymology*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002786:Cholesterol Side-Chain Cleavage Enzyme / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D005260:Female; D006041:Goats; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012756:Sheep
chemical_list: D018076:DNA, Complementary; D002786:Cholesterol Side-Chain Cleavage Enzyme