title: Augmentation of tumor metastasis by platelet-activating factor.
abstract: The effect of platelet-activating factor (PAF) on experimental pulmonary metastasis by the B16F10 murine melanoma and the possible involvement of PAF in the activities of tumor necrosis factor alpha (TNF-alpha) and interleukin 1alpha (IL-1alpha) in tumor metastasis were investigated. i.p. injection of PAF enhanced the lung colonization in a dose- and time-dependent manner. PAF enhanced lung colonization when it was administered after, but not before, B16F10 inoculation. Multiple injections of PAF were more effective than a single injection. Neutralization of endogenous PAF with PAF antagonist BN50739 decreased lung colonization, suggesting that endogenous PAF plays an important role in pulmonary metastases. A single i.p. injection of TNF-alpha or IL-1alpha caused a marked enhancement in lung colonization. TNF-alpha- and IL-1alpha-mediated enhancement in lung colonies was significantly inhibited by BN50739. These results demonstrate that PAF has a metastasis-enhancing effect and is a mediator of the metastatic activities of TNF-alpha and IL-1alpha.
authors.affiliation: Department of Microbiology, Chunnam National University, Kwangju, Republic of Korea.
authors.forename: S Y
authors.initials: SY
authors.lastname: Im
authors.forename: H M
authors.initials: HM
authors.lastname: Ko
authors.forename: J W
authors.initials: JW
authors.lastname: Kim
authors.forename: H K
authors.initials: HK
authors.lastname: Lee
authors.forename: T Y
authors.initials: TY
authors.lastname: Ha
authors.forename: H B
authors.initials: HB
authors.lastname: Lee
authors.forename: S J
authors.initials: SJ
authors.lastname: Oh
authors.forename: S
authors.initials: S
authors.lastname: Bai
authors.forename: K C
authors.initials: KC
authors.lastname: Chung
authors.forename: Y B
authors.initials: YB
authors.lastname: Lee
authors.forename: H S
authors.initials: HS
authors.lastname: Kang
authors.forename: S B
authors.initials: SB
authors.lastname: Chun
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D001381:Azepines / Q000494:pharmacology; D005260:Female; D007375:Interleukin-1 / Q000494:pharmacology; D008175:Lung Neoplasms / Q000556:secondary; D008546:Melanoma, Experimental / Q000473:pathology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009362:Neoplasm Metastasis; D010972:Platelet Activating Factor / Q000037:antagonists & inhibitors / Q000502:physiology*; D014230:Triazoles / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
chemical_list: D000970:Antineoplastic Agents; D001381:Azepines; D007375:Interleukin-1; D010972:Platelet Activating Factor; D014230:Triazoles; D014409:Tumor Necrosis Factor-alpha; C064509:BN 50739