Cloning of a gene encoding a DNase I-like endonuclease in the human Xq28 region.
To contribute to the isolation of genes within the q-24-qter region of the human X chromosome,we screened three cDNA libraries (human fetal brain, liver and skeletal muscle) with a cosmid clone containing a CpG island previously mapped in the q28 region. A full-length 2.1-kb cDNA clone was isolated (XIB); DNA databank searches revealed identity with an EST fragment (XAP-1), residing between the RCP/GCP and G6PD loci. The XIB coding region (909 bp) showed 44% amino acid (aa) identity to pig DNase I. Several conserved residues have been observed between these two genes including aa in the active site. XIB expressed a single transcript in adult heart and skeletal muscle, whereas, in some fetal tissues, two different-sized transcripts were seen. Zoo blot analysis showed a remarkable cross-species conservation. Expression and sequence of this novel gene are reported.
Pergolizzi
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R
Istituto Tecnologie Biomediche Avanzate, Milano, Italy.
Appierto
V
V
Bosetti
A
A
DeBellis
G L
GL
Rovida
E
E
Biunno
I
I
D000328:Adult; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D016384:Consensus Sequence; D017124:Conserved Sequence; D003360:Cosmids; D018076:DNA, Complementary; D003850:Deoxyribonuclease I / Q000096:biosynthesis / Q000235:genetics*; D005333:Fetus; D005399:Fishes; D006801:Humans; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000201:enzymology; D009206:Myocardium / Q000201:enzymology; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D014158:Transcription, Genetic; D014960:X Chromosome
D018076:DNA, Complementary; D003850:Deoxyribonuclease I