Detection of primary direct and indirect human anti-porcine T cell responses using a porcine dendritic cell population.
The pathways of human anti-pig T cell xenorecognition have been investigated. Freshly isolated porcine alveolar lavage (AL) cells induced primary proliferative responses by human peripheral and cord blood mononuclear cells which were inhibited by anti-HLA-DR antibody (indirect xenorecognition). Following over-night culture, the AL cells acquired the capacity to stimulate proliferation by purified human T cells which was inhibited by anti-SLA-DR antibody (direct xenorecognition). The marked increase in immunogenicity in the porcine AL cells was accompanied by a phenotypic change consistent with dendritic cell maturation. Limiting dilution assays indicate that the total anti-pig T cell response, in particular that mediated by indirect xenorecognition, is stronger than comparable alloresponses.
Dorling
A
A
Department of Immunology, Royal Postgraduate Medical School, Hammersmith Hospital, London, GB.
Lombardi
G
G
Binns
R
R
Lechler
R I
RI
D000818:Animals; D015478:Antigens, Heterophile / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000502:physiology*; D006801:Humans; D013045:Species Specificity; D013552:Swine; D014183:Transplantation, Heterologous
D015478:Antigens, Heterophile