The human cysteine-rich secretory protein (CRISP) family. Primary structure and tissue distribution of CRISP-1, CRISP-2 and CRISP-3.
We report the isolation and characterisation of cDNAs encoding three different, human members of the cysteine-rich secretory protein (CRISP) family. The novel CRISP-1 exists in five cDNA subtypes differing by the presence or absence of a stretch coding for a C-terminal cysteine-rich domain so far found in all members of the family, and by the length of their 3'-untranslated region. CRISP-2 cDNA corresponds to the previously described TPX1 form, with so far unreported 5'-untranslated sequence heterogeneities while CRISP-3 cDNA codes for a new, unique protein. Northern blot analysis of various human organs indicates that CRISP-1 transcripts are epididymis-specific whereas CRISP-2/TPX1 transcripts are detected mainly in the testis and also in the epididymis. CRISP-3 transcripts are more widely distributed and found predominantly in the salivary gland, pancreas and prostate, and in less abundance in the epididymis, ovary, thymus and colon. A protein reacting with an anti-mouse CRISP-1 antibody was isolated from human epididymal extracts and N-terminal sequencing revealed that it corresponded to the CRISP-1 cDNA we have isolated. In contrast to findings on its rat counterpart epididymal protein DE/acidic epididymal glycoprotein (AEG), no significant association of CRISP-1 with human spermatozoa was observed.
Krätzschmar
J
J
Research Laboratories of Schering AG, Berlin, Germany.
Haendler
B
B
Eberspaecher
U
U
Roosterman
D
D
Donner
P
P
Schleuning
W D
WD
D000368:Aged; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015815:Cell Adhesion Molecules; D018076:DNA, Complementary; D004797:Enzyme-Linked Immunosorbent Assay; D004822:Epididymis / Q000378:metabolism*; D015723:Gene Library; D006023:Glycoproteins / Q000032:analysis / Q000096:biosynthesis* / Q000737:chemistry*; D006801:Humans; D008297:Male; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D051379:Mice; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D009928:Organ Specificity; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D051381:Rats; D011994:Recombinant Proteins / Q000032:analysis / Q000096:biosynthesis / Q000737:chemistry; D012471:Salivary Proteins and Peptides / Q000032:analysis / Q000096:biosynthesis* / Q000737:chemistry*; D029589:Seminal Plasma Proteins; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D013094:Spermatozoa / Q000378:metabolism; D013737:Testis / Q000378:metabolism*; D014158:Transcription, Genetic
C103538:CRISP1 protein, human; C062621:CRISP2 protein, human; C489926:CRISP3 protein, human; D015815:Cell Adhesion Molecules; C486321:Crisp1 protein, mouse; C483816:Crisp2 protein, mouse; C489927:Crisp3 protein, mouse; C486320:Crisp3 protein, rat; D018076:DNA, Complementary; D006023:Glycoproteins; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger; D011994:Recombinant Proteins; D012471:Salivary Proteins and Peptides; D029589:Seminal Plasma Proteins