In vivo effect of prednisolone on release of leukotriene B4 from neutrophils from asthmatic patients.
We examined the release of leukotriene B4 from calcium ionophore A23187-stimulated neutrophils from asthmatic patients treated with or without intravenous prednisolone during an asthmatic attack. The mean level of LTB4 in the supernatant of stimulated neutrophils from patients treated with intravenous prednisolone was significantly lower than that in the supernatant of stimulated neutrophils from those without prednisolone treatment. Preincubation with prednisolone caused a dose-dependent inhibition of LTB4 release from calcium ionophore A23187-stimulated neutrophils. These findings suggest that intravenous prednisolone inhibits the release of LTB4 from neutrophils in vivo.
Shindo
K
K
First Department of Internal Medicine, Yokohama City University School of Medicine, Japan.
Koide
K
K
Fukumura
M
M
Hirai
Y
Y
D000328:Adult; D001249:Asthma / Q000378:metabolism*; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000502:physiology; D002478:Cells, Cultured; D006801:Humans; D007476:Ionophores / Q000494:pharmacology; D007958:Leukocyte Count / Q000187:drug effects; D007975:Leukotriene B4 / Q000378:metabolism*; D008875:Middle Aged; D009504:Neutrophils / Q000378:metabolism*; D011239:Prednisolone / Q000494:pharmacology*; D012636:Secretory Rate / Q000187:drug effects
D007476:Ionophores; D007975:Leukotriene B4; D000001:Calcimycin; D011239:Prednisolone; D002118:Calcium