Flow cytometric analysis using lipophilic dye PKH-2 for adhesion of Vibrio cholerae to Intestine 407 cells.
A comparative study of indirect and direct flow cytometric analysis for adherence of Vibrio cholerae to Intestine 407 cells was performed. The direct flow cytometric analysis employed the lipophilic dye PKH-2. It was concluded that direct flow cytometry using the lipophilic dye PKH-2 is useful and convenient for analyzing bacterium-host cell interactions, since it does not require any specific antibody as the first antibody.
Taguchi
H
H
Department of Microbiology, Kyorin University School of Medicine, Tokyo, Japan.
Osaki
T
T
Yamaguchi
H
H
Kamiya
S
S
D001422:Bacterial Adhesion; D002469:Cell Separation; D002478:Cells, Cultured; D004848:Epithelium / Q000382:microbiology; D005434:Flow Cytometry / Q000379:methods*; D005456:Fluorescent Dyes; D006801:Humans; D007413:Intestinal Mucosa / Q000382:microbiology*; D009930:Organic Chemicals; D014734:Vibrio cholerae / Q000502:physiology*
D005456:Fluorescent Dyes; D009930:Organic Chemicals; C068241:PKH-2