Effect of Different Temperature Upshifts on Protein Synthesis by the Psychrotrophic Bacterium Pseudomonas fragi
Pseudomonas fragi, a psychrotroph bacterium involved in meat product spoilage, was shifted either from 5degrees to 20degreesC or 30degreesC and from 28degrees to 34degreesC. The heat-shocked cells in the mid-log phase rapidly reached the characteristic growth rate of the postshock temperature. The patterns of synthesized proteins were compared by autoradiography of two-dimensional gel electrophoregrams. The rates of synthesis, after transfer of cells from 5degrees to 30degreesC, 5degrees to 20degreesC, and 28degrees to 34degreesC, changed for 30, 26, and 21 proteins respectively, of which 19, 17, and 12 were increased respectively. Thirteen proteins changed similarly for the three treatments, and two of the seven overexpressed proteins were immunologically related to the Escherichia coli DnaK and GroEL heat shock proteins. From the four low-molecular-mass proteins, belonging to the family of DNA-binding cold shock proteins (CSPs) such as CS7.4, the major E. coli CSP [15], the amounts of C7.0 and C8.0 decreased rapidly after the upshifts, whereas that of E7.0 and E8.0 increased greatly.
Michel
V
Station de Recherches sur la Viande, Unite de Recherches de Microbiologie, Institut National de la Recherche Agronomique de Theix, 63122 Saint-Genes-Champanelle, France
Labadie
J
Hebraud
M