Unilateral enucleation induces an increase of 160 kd neurofilament in lateral geniculate nuclei synapses.
Ultrastructural synaptic changes of retinal origin in the pars dorsalis lateral geniculate nuclei (dLGN) after enucleation have been studied in this laboratory, showing a filamentous hypertrophy with maximal expression at 4-6 days post-lesion in monkeys (Pecci Saavedra et al., 1970, 1971). The aim of this work was to elucidate the nature of the newly formed filament in dLGN in post-enucleated rats. Male Wistar rats were fixed with 4% paraformaldehyde plus 0.25% glutaraldehyde in 0.1M phosphate buffer, through the abdominal aorta after 3, 5, and 7 days postenucleation. Sections obtained were incubated with antibodies to the phosphorylated portion of the 160 Kd neurofilaments (1:3000) and anti-GFAP (1:25000). There was an increase in 160 Kd neurofilament staining in axons and degenerating nerve endings in dLGN, as well as a typical astroglial immunostained reaction. Our results show that the newly formed neurofilaments after deafferentation are of the 160 Kd type, commonly present in normal axons.
Capani
F
F
Instituto de Biología Celular y Neurociencias Prof. Eduardo De Robertis, Facultad de Medicina, Universidad de Buenos Aires, República Argentina.
Loidl
C F
CF
Pecci Saavedra
J
J
D000818:Animals; D001253:Astrocytes / Q000737:chemistry; D015353:Eye Enucleation; D005829:Geniculate Bodies / Q000737:chemistry* / Q000166:cytology; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D008297:Male; D009410:Nerve Degeneration / Q000502:physiology; D016900:Neurofilament Proteins / Q000032:analysis / Q000378:metabolism*; D009474:Neurons / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure; D051381:Rats; D017208:Rats, Wistar; D013569:Synapses / Q000737:chemistry* / Q000378:metabolism
D005904:Glial Fibrillary Acidic Protein; D016900:Neurofilament Proteins