Porcine polypyrimidine tract-binding protein stimulates translation initiation at the internal ribosome entry site of foot-and-mouth-disease virus.
The cDNA for porcine polypyrimidine tract-binding protein (sPTB) was cloned. The sPTB amino acid sequence is highly homologous to the human PTB sequence (97% identity), and the sPTB sequence corresponds to that of the longest human PTB, PTB4. The specificity of binding in the UV-crosslink of sPTB to the internal ribosome entry site (IRES) of foot-and-mouth-disease virus (FMDV) is similar to that of human PTB. Purified recombinant sPTB efficiently stimulates internal translation initiation directed by the FMDV IRES in a rabbit reticulocyte lysate translation system from which the internal PTB had been depleted.
Niepmann
M
M
Institut für Biochemie, Giessen, Germany.
D000595:Amino Acid Sequence; D000818:Animals; D005537:Aphthovirus / Q000235:genetics*; D001483:Base Sequence; D001665:Binding Sites; D003001:Cloning, Molecular; D003432:Cross-Linking Reagents; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000494:pharmacology / Q000502:physiology*; D006801:Humans; D008969:Molecular Sequence Data; D010442:Peptide Chain Initiation, Translational / Q000187:drug effects / Q000502:physiology*; D038941:Polypyrimidine Tract-Binding Protein; D012367:RNA, Viral / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000235:genetics* / Q000494:pharmacology / Q000502:physiology*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D012045:Regulatory Sequences, Nucleic Acid / Q000502:physiology*; D012270:Ribosomes; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013552:Swine; D014466:Ultraviolet Rays
D003432:Cross-Linking Reagents; D004268:DNA-Binding Proteins; D012367:RNA, Viral; D016601:RNA-Binding Proteins; D011993:Recombinant Fusion Proteins; D038941:Polypyrimidine Tract-Binding Protein