Structure of the O-specific polysaccharide of an Aeromonas trota strain cross-reactive with Vibrio cholerae O139 Bengal.
The O-specific polysaccharide of an Aeromonas trota strain was isolated by hydrolysis of the lipopolysaccharide at pH 4.5 followed by gel-permeation chromatography and found to consist of hexasaccharide repeating units containing D-galactose, L-rhamnose, 3,6-dideoxy-L-xylo-hexose (colitose, Col), 2-acetamido-2-deoxy-D-glucose and 2-acetamido-2-deoxy-D-galactose in the ratios 1:1:2:1:1. Partial hydrolysis of the polysaccharide with 48% hydrofluoric acid resulted in selective removal of colitose to give a modified polysaccharide containing the other four sugar constituents. On the basis of methylation analysis and NMR spectroscopic studies of the initial and modified, colitose-free polysaccharide, it was concluded that the repeating unit of the O-specific polysaccharide has the following structure [sequence: see text] The known cross-reactivity between the strain studied and Vibrio cholerae O139 Bengal is substantiated by the presence of a common colitose-containing epitope shared by the O-specific polysaccharide of A. trota and the capsular polysaccharide of V. cholerae, which is thought to carry determinants of O-specificity.
Knirel
Y A
YA
Karolinska Institute, Clinical Research Center, Huddinge University Hospital, Sweden.
Senchenkova
S N
SN
Jansson
P E
PE
Weintraub
A
A
Ansaruzzaman
M
M
Albert
M J
MJ
D000333:Aeromonas / Q000737:chemistry*; D002240:Carbohydrate Sequence; D000939:Epitopes / Q000737:chemistry; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008745:Methylation; D008969:Molecular Sequence Data; D019081:O Antigens / Q000737:chemistry* / Q000276:immunology* / Q000378:metabolism; D014734:Vibrio cholerae / Q000737:chemistry / Q000276:immunology*
D000939:Epitopes; D019081:O Antigens