Restriction endonucleases induce chromosomal aberrations in barley.
The clastogenic ability of the restriction endonucleases (MspI, HpaII and HaeIII) in germinating seeds of reconstructed barley karyotype was assessed. An effective induction of chromosomal aberrations after restrictase treatment was observed. The frequency, types and cell-cycle dependence of the observed abnormalities are discussed in relation to the distinct characteristics of the enzymes and the features of the plant genome. The capacity to induce aberrations was not significantly influenced by the nature of the double-strand breaks (blunt- or cohesive-ended); however, it was dependent on the methylation status of the plant DNA. The restriction enzymes displayed an S-independent mode of action revealing the transition between G1 and S as the most sensitive stage of the cell cycle in barley for induction of chromosomal damage.
Stoilov
L M
LM
Institute of Genetics Doncho Kostoff, Bulgarian Academy of Sciences, Kostinbrod, Bulgaria.
Mirkova
V N
VN
Dimitrova
A
A
Uzunova
V
V
Gecheff
K I
KI
D001483:Base Sequence; D002453:Cell Cycle; D002869:Chromosome Aberrations; D002875:Chromosomes / Q000187:drug effects / Q000648:ultrastructure; D004249:DNA Damage; D004262:DNA Restriction Enzymes / Q000633:toxicity*; D018744:DNA, Plant / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D019003:Deoxyribonuclease HpaII / Q000633:toxicity; D015252:Deoxyribonucleases, Type II Site-Specific / Q000633:toxicity; D001467:Hordeum / Q000166:cytology / Q000187:drug effects* / Q000235:genetics*; D008745:Methylation; D009153:Mutagens / Q000633:toxicity*
D018744:DNA, Plant; D009153:Mutagens; D004262:DNA Restriction Enzymes; D019003:Deoxyribonuclease HpaII; D015252:Deoxyribonucleases, Type II Site-Specific; C024403:GGCC-specific type II deoxyribonucleases