title: Effects of free fatty acids on the metabolic response to oral fructose in lean healthy humans.
abstract: OBJECTIVE
  To study the effects of an experimental increase in plasma FFA concentration on fructose to glucose conversion, total hepatic glucose output and glycaemic response to oral fructose.
  
  
  METHODS
  Six healthy subjects (three men, three women; age: 24.3 +/- 2.3 years; BMI: 21.6 +/- 0.8 kg/m2).
  
  
  METHODS
  Each subject absorbed 0.5 g/kg of 13C-enriched fructose and randomly received either a triglyceride-heparin infusion or saline.
  
  
  METHODS
  Total hepatic glucose output was traced with 6,6-2H2-glucose. Appearance in plasma of glucose synthesized from fructose was calculated from the isotopic enrichment in 13C of plasma glucose. Substrates oxidation was assessed with indirect calorimetry.
  
  
  RESULTS
  The triglycerides-heparin infusion increased FFA concentration before fructose as compared to saline (1086 +/- 40 vs 451 +/- 67 microM; p < 0.001) and lipid oxidation was 15% and 70% increased before and during fructose, respectively as compared to saline. Total hepatic glucose output, plasma appearance of glucose synthesized from fructose and glycaemic response were not affected. Glycogen storage over the first 3 h following fructose was increased (6.2 +/- 2.1 g vs 0.3 +/- 2.1 g; p < 0.01).
  
  
  CONCLUSIONS
  Triglycerides-heparin infusion did not stimulate plasma glucose appearance from fructose. Liver glucose-6-phosphate could have been produced in excess and diverted towards glycogen synthesis.
authors.affiliation: Laboratoire de Nutrition et Jeune Equipe 313 Lipides et Croissance Hôpital Bretonneau, Tours, France.
authors.forename: J
authors.initials: J
authors.lastname: Delarue
authors.forename: S
authors.initials: S
authors.lastname: Normand
authors.forename: C
authors.initials: C
authors.lastname: Couet
authors.forename: C
authors.initials: C
authors.lastname: Pachiaudi
authors.forename: C
authors.initials: C
authors.lastname: Urbain
authors.forename: F
authors.initials: F
authors.lastname: Lamisse
authors.forename: J P
authors.initials: JP
authors.lastname: Riou
mesh_terms: D000328:Adult; D001786:Blood Glucose / Q000378:metabolism; D002153:Calorimetry, Indirect; D003903:Deuterium; D005230:Fatty Acids, Nonesterified / Q000097:blood / Q000494:pharmacology*; D005260:Female; D005632:Fructose / Q000008:administration & dosage / Q000494:pharmacology*; D005947:Glucose / Q000378:metabolism; D006003:Glycogen / Q000378:metabolism; D006493:Heparin / Q000494:pharmacology; D006801:Humans; D007700:Kinetics; D015227:Lipid Peroxidation; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D014280:Triglycerides / Q000494:pharmacology
chemical_list: D001786:Blood Glucose; D005230:Fatty Acids, Nonesterified; D014280:Triglycerides; D005632:Fructose; D006493:Heparin; D006003:Glycogen; D003903:Deuterium; D005947:Glucose