Dehydrogenase binding to the 3'-untranslated region of GLUT1 mRNA.
Employing RNA gel mobility shift assays we detected specific binding events between several dehydrogenases and various regions of the GLUT1 mRNA 3'-untranslated region. In particular, the enzymes glyceraldehyde 3-phosphate dehydrogenase (G3PDH), lactate dehydrogenase (LDH), and glucose 6-phosphate dehydrogenase (G6PDH) bound to the GLUT1 3'-UTR while isocitrate dehydrogenase (IDH) and glutamate dehydrogenase (GluDH) did not. Comparison of shifts obtained with purified dehydrogenases to those obtained using authentic cell extracts indicate that G3PDH and G6PDH may play a role in the intact cell.
McGowan
K
K
Department of Biochemistry, East Carolina University, School of Medicine, Greenville, North Carolina 27834, USA.
Pekala
P H
PH
D016475:3T3 Cells; D000818:Animals; D001483:Base Sequence; D004247:DNA; D005947:Glucose / Q000378:metabolism; D051272:Glucose Transporter Type 1; D051379:Mice; D008969:Molecular Sequence Data; D009004:Monosaccharide Transport Proteins / Q000235:genetics*; D010088:Oxidoreductases / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D013379:Substrate Specificity
D051272:Glucose Transporter Type 1; D009004:Monosaccharide Transport Proteins; D012333:RNA, Messenger; C495678:Slc2a1 protein, mouse; D004247:DNA; D010088:Oxidoreductases; D005947:Glucose