Generation and mapping of Mus spretus strain-specific markers for rapid genomic scanning.
We describe here a set of genetic markers, based on IRS-PCR amplification difference, that are specifically designed for efficient, high throughput genetic mapping in [(M. domesticus x wild-derived) F1 x M. domesticus] interspecific backcrosses. 146 new genetic loci have been mapped, and strain distribution for these markers has been determined in 96 mouse strains. 103 (81%) of 127 tested markers are present only in one or more wild-derived strains, but absent in 76 other commonly used strains, demonstrating their utility in a variety of mouse pair combinations. Because of the ease of genotyping with this marker set, rapid genome scans for complex genetic trait loci involving crosses between wild-derived strains and other commonly used strains can now be carried out efficiently with large numbers of animals.
Elango
R
R
Center for Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
Riba
L
L
Housman
D
D
Hunter
K
K
D000818:Animals; D002874:Chromosome Mapping / Q000379:methods*; D003433:Crosses, Genetic; D005260:Female; D005819:Genetic Markers; D016678:Genome; D006824:Hybridization, Genetic; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL / Q000235:genetics; D008815:Mice, Inbred Strains / Q000235:genetics; D009115:Muridae / Q000235:genetics*; D016133:Polymerase Chain Reaction; D013382:Subtraction Technique
D005819:Genetic Markers