Physical mapping of the bloom syndrome region by the identification of YAC and P1 clones from human chromosome 15 band q26.1.
The gene for Bloom syndrome (BLM) has been mapped to human chromosome 15 band q26.1 by homozygosity mapping. Further refinement of the location of BLM has relied upon linkage-disequilibrium mapping and somatic intragenic recombination. In combination with these mapping approaches and to identify novel DNA markers and probes for the BLM candidate region, a contiguous representation of the 2-Mb region that contains the BLM gene was generated and is presented here. YAC and P1 clones from the region have been identified and ordered by using previously available genetic markers in the region along with newly developed sequence-tagged sites from radiation-reduced hybrids, polymorphic dinucleotide repeat loci, and end sequences of YACs and P1s. A long-range restriction map of the 2-Mb region that allowed estimation of the distance between polymorphic microsatellite loci is also reported. This map and the DNA markers derived from it were instrumental in the recent identification of the BLM gene.
Straughen
J
J
Department of Molecular Genetics, Biochemistry and Microbiology, University of Cincinnati College of Medicine, Cincinnati, Ohio, 45267-0524, USA.
Ciocci
S
S
Ye
T Z
TZ
Lennon
D N
DN
Proytcheva
M
M
Alhadeff
B
B
Goodfellow
P
P
German
J
J
Ellis
N A
NA
Groden
J
J
D000251:Adenosine Triphosphatases / Q000235:genetics*; D000818:Animals; D017101:Bacteriophage P1 / Q000235:genetics*; D001483:Base Sequence; D001816:Bloom Syndrome / Q000235:genetics*; D016466:CHO Cells; D002265:Carboxylic Ester Hydrolases / Q000235:genetics; D002874:Chromosome Mapping; D018244:Chromosomes, Artificial, Yeast / Q000235:genetics*; D002884:Chromosomes, Human, Pair 15 / Q000235:genetics* / Q000648:ultrastructure; D003241:Consanguinity; D006224:Cricetinae; D004265:DNA Helicases / Q000235:genetics*; D005808:Genes, Recessive; D006801:Humans; D006822:Hybrid Cells; D017404:In Situ Hybridization, Fluorescence; D015810:Linkage Disequilibrium; D018895:Microsatellite Repeats; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011505:Protein-Tyrosine Kinases; D011518:Proto-Oncogene Proteins; D051578:Proto-Oncogene Proteins c-fes; D011519:Proto-Oncogenes; D053484:RecQ Helicases
D011518:Proto-Oncogene Proteins; D011505:Protein-Tyrosine Kinases; C496645:FES protein, human; D051578:Proto-Oncogene Proteins c-fes; D002265:Carboxylic Ester Hydrolases; D000251:Adenosine Triphosphatases; C097151:Bloom syndrome protein; D004265:DNA Helicases; D053484:RecQ Helicases