Effect of mutation of potassium-efflux system, KefA, on mechanosensitive channels in the cytoplasmic membrane of Escherichia coli.
The effect of a kefA mutation on the mechanosensitive channels in the cytoplasmic membrane of Escherichia coli was established by introducing a mutation of the kefA gene into wild-type E. coli by P1 transduction. The mutation of the kefA gene not only made the cells sensitive to K+ in the medium but also changed the mechanosensitive channel activity. The kefA mutation did not change the conductances of the two mechanosensitive channels in the cytoplasmic membrane of E. coli, but it prolonged the channel open time. Also, the kefA mutation made the cells more sensitive to pressure in comparison to wild-type cells. The high sensitivity to pressure of the kefA mutant was not modulated by betaine or by the potassium gradient across the membrane. The effect of the kefA mutation on mechanosensitive channels was not due to a membrane fluidity change. KefA might be a regulator for mechanosensitive channels.
Cui
C
C
Department of Biochemistry, University of Wisconsin-Madison 53706, USA.
Adler
J
J
D001696:Biomechanical Phenomena; D002462:Cell Membrane / Q000378:metabolism; D004553:Electric Conductivity; D004926:Escherichia coli / Q000378:metabolism*; D007473:Ion Channels / Q000235:genetics / Q000378:metabolism*; D008560:Membrane Fluidity; D009154:Mutation; D011188:Potassium / Q000378:metabolism*; D011312:Pressure
D007473:Ion Channels; D011188:Potassium