Correlations of Ki-67 and PCNA to DNA ploidy, S-phase fraction and survival in uveal melanoma.
In 79 patients with uveal melanoma, the tumours were investigated by DNA flow cytometry and immunohistochemical staining of PCNA and Ki-67. S-phase as a continuous variable was significantly correlated with Ki-67 (P = 0.033), but not with PCNA. DNA ploidy was not correlated with either of the two antigens. Ki-67 was significantly correlated with histopathological type (P < 0.001) and tumour size (P < 0.001). Large tumours and epithelioid cell type were associated with a high frequency of Ki-67 positive cells. A high level of Ki-67 positivity (> or = 6.5%) was also associated with a shorter survival (P = 0.0037), and when adjusted for DNA ploidy, histopathological type and tumour size, Ki-67 in the multivariate analysis remained an important prognostic factor (P = 0.017).
Karlsson
M
M
Department of Oncology, University Hospital, Sweden.
Boeryd
B
B
Carstensen
J
J
Frånlund
B
B
Gustafsson
B
B
Kågedal
B
B
Sun
X F
XF
Wingren
S
S
D000951:Antigens, Neoplasm / Q000032:analysis*; D002455:Cell Division; D005434:Flow Cytometry; D006801:Humans; D007124:Immunoenzyme Techniques; D019394:Ki-67 Antigen; D008545:Melanoma / Q000235:genetics / Q000276:immunology / Q000473:pathology*; D009363:Neoplasm Proteins / Q000032:analysis*; D009687:Nuclear Proteins / Q000032:analysis*; D011003:Ploidies; D011379:Prognosis; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis*; D012307:Risk Factors; D016196:S Phase; D015996:Survival Rate; D014604:Uveal Neoplasms / Q000235:genetics / Q000276:immunology / Q000473:pathology*
D000951:Antigens, Neoplasm; D019394:Ki-67 Antigen; D009363:Neoplasm Proteins; D009687:Nuclear Proteins; D018809:Proliferating Cell Nuclear Antigen