Studies on cooperative binding of an extended distamycin A analogue in the minor groove of DNA by NMR spectroscopy.
An analogue of the DNA minor groove binding antibiotic distamycin A, P4+, was found to bind in a 2:1 cooperative manner into the narrow groove of oligodeoxybibonucleotide d(CGTATATACG)2 by 1D-NMR titration. The resulting 2:1 ligand-DNA complex was characterized by 2D NMR experiments including NOESY, COSY, and TOCSY. Two molecules are oriented side by side opposite to each other; each has the N terminus to C terminus direction parallel to the 5' to 3' direction of its adjacent DNA strand. This study corroborates our previous CD and ethidium fluorometry results and provides new support for the generality of the antiparallel side by side binding motif first observed with distamycin A.
Yang
Y
Y
Department of Chemistry, University of Alberta, Edmonton, Canada.
Chen
Y H
YH
Pon
R T
RT
Lown
J W
JW
D000900:Anti-Bacterial Agents / Q000737:chemistry; D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D004214:Distamycins / Q000737:chemistry*; D006860:Hydrogen Bonding; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000737:chemistry
D000900:Anti-Bacterial Agents; D004214:Distamycins; D009838:Oligodeoxyribonucleotides; C030000:stallimycin; D004247:DNA