Molecular characterization of seizure-related genes isolated by differential screening.
To isolate seizure-related genes, we applied differential screening technique to the cDNA library which constructed from primary cultured cerebral cortical cells treated with pentylenetetrazol (PTZ). Northern blotting analysis of mRNA levels in the cerebra after systemic administration of PTZ confirmed the results of differential screening procedure: 6 clones showed increased mRNA level and 3 clones showed decreased expression with PTZ. Interestingly, 4 genes which were isolated by this technique were related to intracellular calcium action. They were cytosolic phospholipase A2, 78 kDa glucose regulated protein, SEZ-15 which has an EF hand motif and PTZ-17 that causes calcium current in Xenopus oocyte with PTZ application. These data and our previous results suggest that intracellular calcium may play an important role for seizure-related pathophysiological changes in neuronal cells.
Kajiwara
K
K
Department of Physiology, Kanagawa Dental College, Yokosuka, Japan.
Nagawawa
H
H
Shimizu-Nishikawa
S
S
Ookuri
T
T
Kimura
M
M
Sugaya
E
E
D000595:Amino Acid Sequence; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002352:Carrier Proteins / Q000096:biosynthesis / Q000235:genetics; D002478:Cells, Cultured; D003001:Cloning, Molecular; D018076:DNA, Complementary; D000091342:Endoplasmic Reticulum Chaperone BiP; D005260:Female; D015870:Gene Expression; D016762:Genes, fos; D006360:Heat-Shock Proteins; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018832:Molecular Chaperones / Q000096:biosynthesis / Q000235:genetics; D008969:Molecular Sequence Data; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D009865:Oocytes / Q000502:physiology; D010433:Pentylenetetrazole / Q000494:pharmacology; D010741:Phospholipases A / Q000096:biosynthesis / Q000235:genetics; D054467:Phospholipases A2; D016760:Proto-Oncogene Proteins c-fos / Q000096:biosynthesis; D011994:Recombinant Proteins / Q000096:biosynthesis; D012640:Seizures / Q000235:genetics* / Q000503:physiopathology; D017385:Sequence Homology; D014981:Xenopus
D002352:Carrier Proteins; D018076:DNA, Complementary; D000091342:Endoplasmic Reticulum Chaperone BiP; D006360:Heat-Shock Proteins; D018832:Molecular Chaperones; D016760:Proto-Oncogene Proteins c-fos; D011994:Recombinant Proteins; D010741:Phospholipases A; D054467:Phospholipases A2; D002118:Calcium; D010433:Pentylenetetrazole