Drosophila photoreceptor axon guidance and targeting requires the dreadlocks SH2/SH3 adapter protein.
Mutations in the Drosophila gene dreadlocks (dock) disrupt photoreceptor cell (R cell) axon guidance and targeting. Genetic mosaic analysis and cell-type-specific expression of dock transgenes demonstrate dock is required in R cells for proper innervation. Dock protein contains one SH2 and three SH3 domains, implicating it in tyrosine kinase signaling, and is highly related to the human proto-oncogene Nck. Dock expression is detected in R cell growth cones in the target region. We propose Dock transmits signals in the growth cone in response to guidance and targeting cues. These findings provide an important step for dissection of signaling pathways regulating growth cone motility.
Garrity
P A
PA
Department of Biological Chemistry, Molecular Biology Institute, Howard Hughes Medical Institute, The School of Medicine, University of California, Los Angeles, California 90024, USA.
Rao
Y
Y
Salecker
I
I
McGlade
J
J
Pawson
T
T
Zipursky
S L
SL
D048868:Adaptor Proteins, Signal Transducing; D000595:Amino Acid Sequence; D000818:Animals; D001369:Axons / Q000378:metabolism; D004330:Drosophila / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D029721:Drosophila Proteins; D005260:Female; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D009030:Mosaicism; D009154:Mutation; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D015513:Oncogene Proteins / Q000235:genetics / Q000378:metabolism; D017956:Photoreceptor Cells, Invertebrate / Q000254:growth & development / Q000378:metabolism*; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D000090063:Proto-Oncogene Mas; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction; D018909:src Homology Domains
D048868:Adaptor Proteins, Signal Transducing; D029721:Drosophila Proteins; C000715470:MAS1 protein, human; C078332:Nck protein; D009419:Nerve Tissue Proteins; D015513:Oncogene Proteins; D000090063:Proto-Oncogene Mas; C099753:dock protein, Drosophila; D011505:Protein-Tyrosine Kinases