Active complex formation of type I and type II activin and TGF beta receptors in vivo as studied by overexpression in zebrafish embryos.
We have investigated the involvement of activin receptors and TGF beta type I receptor in zebrafish development. Overexpression of either full-length or a truncated form of mouse ActR-IIA interferes with the development. Different splice variants of mouse ActR-IIB have distinct effects; ActR-IIB4 induces abnormal embryos, whereas ActR-IIB2 does not. Activin and TGF beta type I receptors can induce axis duplications. Co-expression of ActR-IA or ActR-IB with the type II activin receptors results in a synergistic increase of the frequency of axis duplication. Moreover, ActR-IIB2 is synergistic with ActR-IA and ActR-IB, demonstrating that ActR-IIB2 can interact with the zebrafish ligand. Overexpression of TGF beta R-I with ActR-IIA or ActR IIB4 results in a synergistic increase in frequency of abnormal embryos, whereas in combination with ActR-IIB2 no such increase occurs.
de Vries
C J
CJ
Hubrecht Laboratory, Netherlands Institute for Developmental Biology, Utrecht, The Netherlands.
de Boer
J
J
Joore
J
J
Strähle
U
U
van Achterberg
T A
TA
Huylebroeck
D
D
Verschueren
K
K
Miyazono
K
K
van den Eijnden-van Raaij
A J
AJ
Zivkovic
D
D
D028341:Activins; D000818:Animals; D001483:Base Sequence; D007265:Inhibins / Q000235:genetics / Q000378:metabolism*; D008648:Mesoderm; D051379:Mice; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D011485:Protein Binding; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D012313:RNA / Q000008:administration & dosage; D018125:Receptors, Transforming Growth Factor beta / Q000235:genetics / Q000378:metabolism*; D015027:Zebrafish / Q000196:embryology* / Q000235:genetics
D009838:Oligodeoxyribonucleotides; D018125:Receptors, Transforming Growth Factor beta; D028341:Activins; D007265:Inhibins; D012313:RNA; D017346:Protein Serine-Threonine Kinases