title: Pregnancy resulting from intracytoplasmic injection of cryopreserved spermatozoa recovered from testicular biopsy.
abstract: We report a clinical pregnancy occurring in a 31 year old patient following intracytoplasmic sperm injection (ICSI) of cryopreserved spermatozoa obtained from a testicular biopsy. This was the couple's second attempt at an ovarian stimulated cycle resulting in the collection of 17 metaphase II ova which were all injected with progressively motile spermatozoa. A fertilization rate of 58% and a cleavage rate of 90% were achieved. This report is our first case of ICSI using cryopreserved testicular spermatozoa which resulted in normal fertilization, embryo development and an on-going singleton pregnancy.
authors.affiliation: Lingard Fertility Centre, 23 Merewether Street, Merewether, NSW, Australia 2291.
authors.forename: B T
authors.initials: BT
authors.lastname: Podsiadly
authors.forename: R J
authors.initials: RJ
authors.lastname: Woolcott
authors.forename: J D
authors.initials: JD
authors.lastname: Stanger
authors.forename: K
authors.initials: K
authors.lastname: Stevenson
mesh_terms: D000328:Adult; D001706:Biopsy; D002469:Cell Separation / Q000379:methods; D015925:Cryopreservation; D003593:Cytoplasm; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D006801:Humans; D008297:Male; D008845:Microinjections; D009845:Oligospermia / Q000473:pathology; D009865:Oocytes / Q000166:cytology; D011247:Pregnancy; D013737:Testis / Q000166:cytology*