Platelet-mediated lymphocyte delivery to high endothelial venules.
Circulating lymphocytes gain access to lymph nodes owing to their ability to initiate rolling along specialized high endothelial venules (HEVs). One mechanism of rolling involves L-selectin binding to peripheral node addressin (PNAd) on HEVs. Activated platelets are shown to bind to circulating lymphocytes and to mediate rolling in HEVs, in vivo, through another molecule, P-selectin, which also interacts with PNAd. In vitro, activated platelets enhanced tethering of lymphocytes to PNAd and sustained lymphocyte rolling, even in the absence of functional L-selectin. Thus, a platelet pathway operating through P-selectin provides a second mechanism for lymphocyte delivery to HEVs.
Diacovo
T G
TG
Center for Blood Research, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA.
Puri
K D
KD
Warnock
R A
RA
Springer
T A
TA
von Andrian
U H
UH
D000818:Animals; D000954:Antigens, Surface / Q000378:metabolism*; D001792:Blood Platelets / Q000502:physiology*; D002448:Cell Adhesion; D002465:Cell Movement; D004730:Endothelium, Vascular / Q000166:cytology; D006801:Humans; D019041:L-Selectin / Q000502:physiology; D008024:Ligands; D008198:Lymph Nodes / Q000098:blood supply* / Q000166:cytology; D008214:Lymphocytes / Q000166:cytology / Q000502:physiology*; D008562:Membrane Glycoproteins / Q000378:metabolism; D008565:Membrane Proteins; D051379:Mice; D019007:P-Selectin / Q000378:metabolism; D015539:Platelet Activation; D016201:Receptors, Lymphocyte Homing / Q000378:metabolism; D014162:Transfection; D014407:Tumor Cells, Cultured; D014699:Venules / Q000166:cytology
D000954:Antigens, Surface; C069886:L-selectin counter-receptors; D008024:Ligands; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D019007:P-Selectin; C084882:P-selectin ligand protein; D016201:Receptors, Lymphocyte Homing; D019041:L-Selectin