v-rel and c-rel induce a membrane protein, p75, on avian hematopoietic cells.
v-rel-induced neoplasias are thought to be caused by the aberrant expression of cellular genes. Using a monoclonal antibody we detected a 75 kDa-protein (p75) on v-rel-transformed chicken hematopoietic cells. This protein is normally expressed only on a certain type of non-T, non-B cells in the chicken caecal tonsils. Overexpression of v-rel in two B-cell lines and in splenic cells induced the expression of p75. In contrast, overexpression of c-rel in these cells resulted in variable expression of p75. These data indicated that p75 is ectopically expressed on v-rel-transformed hematopoietic cells and that it responds differently to v-rel and c-rel induction.
Zhang
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Mary Babb Randolph Cancer Center, West Virginia University, Morgantown 26506-9177, USA.
Humphries
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EH
D000818:Animals; D000911:Antibodies, Monoclonal; D001402:B-Lymphocytes / Q000378:metabolism*; D002461:Cell Line, Transformed; D002642:Chick Embryo; D002645:Chickens; D016393:Lymphoma, B-Cell / Q000378:metabolism; D008565:Membrane Proteins / Q000737:chemistry / Q000378:metabolism*; D008970:Molecular Weight; D011519:Proto-Oncogenes / Q000502:physiology*; D013601:T-Lymphocytes / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D008565:Membrane Proteins