Endothelin stimulates sis-inducing factor-like DNA binding activity in CHO-K1 cells expressing ETA receptors.
ET-1, a member of the family of peptides known as endothelins, binds to a G-protein-coupled receptor, ET(A), and stimulates a variety of cellular responses, including contraction, growth, and mitogenesis. ET-1 stimulation of a chinese hamster ovary cell line stably transfected with the ET(A) receptor (CHO/ET(A)) induced formation of SIF (sis-inducing factor), a key component of the STAT (Signal Transducers and Activators of Transcription) pathway, in a concentration-dependent manner. SIF induction was blocked by a specific inhibitor of ET(A), BQ610, and by genistein, a tyrosine kinase inhibitor. This report demonstrates that ET-1 stimulates the STAT pathway of signal transduction through a G-protein-coupled receptor, ET(A), in this stably transfected cell line.
Peeler
T C
TC
Weis Center for Research, Geisinger Clinic, Danville, Pennsylvania 17822, USA.
Conrad
K M
KM
Baker
K M
KM
D000818:Animals; D001483:Base Sequence; D016466:CHO Cells; D006224:Cricetinae; D015342:DNA Probes; D004268:DNA-Binding Proteins / Q000378:metabolism*; D016232:Endothelins / Q000378:metabolism*; D019833:Genistein; D007529:Isoflavones / Q000494:pharmacology; D008969:Molecular Sequence Data; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors; D044022:Receptor, Endothelin A; D017466:Receptors, Endothelin / Q000235:genetics*
D015342:DNA Probes; D004268:DNA-Binding Proteins; D016232:Endothelins; D007529:Isoflavones; D044022:Receptor, Endothelin A; D017466:Receptors, Endothelin; D019833:Genistein; D011505:Protein-Tyrosine Kinases