title: Bias in the gamma delta T cell response to Listeria monocytogenes. V delta 6.3+ cells are a major component of the gamma delta T cell response to Listeria monocytogenes.
abstract: Despite extensive research, the role that gamma delta T cells play in the immune response to infectious disease has yet to be established. Here we report the generation of a mAb specific for the V delta 6.3 TCR and investigate the gamma delta+ and V delta 6.3+ T cell responses to the intracellular bacterium Listeria monocytogenes in BALB/c mice. By infecting animals with various doses of Listeria and analyzing the components of the cellular immune response at the two primary sites of infection, the liver and spleen, we have shown that the kinetics, composition, and magnitude of the gamma delta and V delta 6.3 T cell responses are dependent upon the injected dose of bacteria and the organ in which the infection is established. At low doses of infection, the gamma delta T cell response occurs late in the disease course, while at high doses, the response is earlier and of greater magnitude, particularly in the liver. At all infectious doses and in both tissues, the V delta 6.3+ population predominates and together with V delta 4+ cells composes the bulk of the gamma delta T cell response. Changes in the morphology of gamma delta+ and V delta 6.3+ cells at the site of infection are consistent with cellular activation and suggest that these cells are active participants in the Listeria-induced immune response. The results of our study suggest that many features of the gamma delta T cell response to Listeria are dose and tissue related.
authors.affiliation: Department of Microbiology, University of Pennsylvania School of Medicine, Philadelphia 19104, USA.
authors.forename: C
authors.initials: C
authors.lastname: Belles
authors.forename: A K
authors.initials: AK
authors.lastname: Kuhl
authors.forename: A J
authors.initials: AJ
authors.lastname: Donoghue
authors.forename: Y
authors.initials: Y
authors.lastname: Sano
authors.forename: R L
authors.initials: RL
authors.lastname: O'Brien
authors.forename: W
authors.initials: W
authors.lastname: Born
authors.forename: K
authors.initials: K
authors.lastname: Bottomly
authors.forename: S R
authors.initials: SR
authors.lastname: Carding
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D001483:Base Sequence; D015169:Colony Count, Microbial; D017931:DNA Primers / Q000235:genetics; D007700:Kinetics; D008089:Listeria monocytogenes / Q000276:immunology*; D008088:Listeriosis / Q000276:immunology / Q000382:microbiology; D008099:Liver / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D051381:Rats; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000235:genetics / Q000378:metabolism*; D013154:Spleen / Q000276:immunology; D016176:T-Lymphocyte Subsets / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D017931:DNA Primers; D016692:Receptors, Antigen, T-Cell, gamma-delta