Activation of a protein tyrosine phosphatase and inactivation of Raf-1 by somatostatin.
Human somatostatin receptor 3 ('hsstr3') was transiently expressed in NIH 3T3 cells stably transformed with Ha-Ras (G12V). Somatostatin activated a protein tyrosine phosphatase and inactivated the constitutively active, membrane-associated form of the Raf-1 serine kinase present in these cells in vivo and in vitro.
Reardon
D B
DB
Howard Hughes Medical Institute, University of Virginia, Charlottesville, VA 22908, U.S.A.
Wood
S L
SL
Brautigan
D L
DL
Bell
G I
GI
Dent
P
P
Sturgill
T W
TW
D016475:3T3 Cells; D000818:Animals; D002461:Cell Line, Transformed; D004789:Enzyme Activation; D011905:Genes, ras; D051379:Mice; D011485:Protein Binding; D017346:Protein Serine-Threonine Kinases / Q000037:antagonists & inhibitors*; D017027:Protein Tyrosine Phosphatases / Q000378:metabolism*; D011518:Proto-Oncogene Proteins / Q000037:antagonists & inhibitors*; D019908:Proto-Oncogene Proteins c-raf; D013004:Somatostatin / Q000378:metabolism / Q000494:pharmacology*
D011518:Proto-Oncogene Proteins; D013004:Somatostatin; D017346:Protein Serine-Threonine Kinases; D019908:Proto-Oncogene Proteins c-raf; D017027:Protein Tyrosine Phosphatases