Over-expression of the NUD1-coded endo-exonuclease in Saccharomyces cerevisiae enhances DNA recombination and repair.
The NUD1(=NUC2) gene of Saccharomyces cerevisiae has been subcloned and over-expressed in multi-copy plasmids. Enhanced expression of this nuclear endo-exonuclease gene was confirmed by Northern hybridization (>10-fold increase), and increased enzymatic activity (2.4-fold increase) was demonstrated by direct immunological assay using antibody raised against the purified Neurospora crassa endo-exonuclease. We found that increased expression of NUD1 was associated with an increase in cell survival after irradiation treatment with gamma rays, and an increase in radiation-induced mitotic recombination frequencies between duplicated gene sequences. The results presented here are consistent with previous biochemical data and confirm a role for the NUD1 gene product in recombination/repair processes.
Sadekova
S
S
Department of Oncology, Division of Radiation-Oncology, McGill University, Montreal General Hospital, 1650 Avenue Cedar, Montreal, Quebec H3G 1A4, Canada.
Chow
T Y
TY
D004260:DNA Repair / Q000235:genetics / Q000502:physiology; D003851:Deoxyribonucleases / Q000235:genetics* / Q000378:metabolism*; D005720:Gamma Rays; D015870:Gene Expression / Q000528:radiation effects; D005800:Genes, Fungal / Q000528:radiation effects; D011995:Recombination, Genetic / Q000528:radiation effects; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism* / Q000528:radiation effects; D029701:Saccharomyces cerevisiae Proteins; D012359:tRNA Methyltransferases
D029701:Saccharomyces cerevisiae Proteins; D012359:tRNA Methyltransferases; D003851:Deoxyribonucleases; C077501:TRM2 protein, S cerevisiae