Interleukin-2 inhibits 3T3 fibroblast proliferation.
In order to clarify the role played by interleukin-2 (IL-2) in the regulation of fibroblast function, we investigated the effect of rat IL-2 and human recombinant IL-2 on 3T3 fibroblast proliferation and collagen synthesis. Fibroblasts were incubated with various concentrations of IL-2 for different periods of time. IL-2 was found to decrease in time- and dose-dependent manner the proliferation of 3T3 fibroblasts. This effect correlated with ability of IL-2 to enhance PGE2 production by 3T3 fibroblasts. When 3T3 fibroblasts were cocultured with rat peritoneal mast cells (MC), the growth-inhibiting effect of IL-2 was significantly less pronounced. Treatment of the cultures with IL-2 had no effect on collagen production by both 3T3 fibroblasts and fibroblasts cocultured with MC. In conclusion, in this study we provide evidence that IL-2, the key cytokine in T-cell growth and differentiation, can affect fibroblast functions.
Rubinchik
E
E
Dept. Pharmacology, School of Pharmacy Hebrew University-Hadassah Medical School, Jerusalem, Israel.
Levi-Schaffer
F
F
D016475:3T3 Cells; D000818:Animals; D002450:Cell Communication; D002455:Cell Division / Q000187:drug effects*; D003094:Collagen / Q000096:biosynthesis; D015232:Dinoprostone / Q000096:biosynthesis; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology*; D007700:Kinetics; D008407:Mast Cells / Q000187:drug effects / Q000502:physiology; D051379:Mice; D051381:Rats; D011994:Recombinant Proteins / Q000494:pharmacology
D007376:Interleukin-2; D011994:Recombinant Proteins; D003094:Collagen; D015232:Dinoprostone