title: [Analysis of the stability of some properties of tick-borne encephalitis virus (strain 205) upon passaging in mice].
abstract: Comparative analysis of the characteristics of tick-borne encephalitis (TBE) strain 205 used for the production of vaccine against TBE and of its variants obtained by passages in mouse brain showed the stability of such properties as infective activity, neurovirulence, sensitivity to physical (heating to 50 C) and chemical (sodium deoxycholate treatment) factors. At the same time increased neurovirulence of variants 205/M10 and 205/M20, which undergone through 10 and 20 passages in white mouse brain, for low-sensitive Syrian hamsters was revealed. Use of a panel of 5 monoclonal antibodies to protein E and of 4 monoclonal antibodies to protein E and of 4 monoclonal antibodies to protein NS3 helped differentiate between not only strains 205 and Sofyin, but between variants of strain 205 as well.
authors.forename: M R
authors.initials: MR
authors.lastname: Karpova
authors.forename: M S
authors.initials: MS
authors.lastname: Vorob'eva
authors.forename: A A
authors.initials: AA
authors.lastname: Kushch
authors.forename: N N
authors.initials: NN
authors.lastname: Kiseleva
authors.forename: M N
authors.initials: MN
authors.lastname: Rasshchepkina
authors.forename: I P
authors.initials: IP
authors.lastname: Ladyzhenskaia
authors.forename: E E
authors.initials: EE
authors.lastname: Mel'nikova
authors.forename: M V
authors.initials: MV
authors.lastname: Shpilevaia
authors.forename: S N
authors.initials: SN
authors.lastname: Amanadze
authors.forename: M F
authors.initials: MF
authors.lastname: Vorovich
mesh_terms: D000818:Animals; D001921:Brain / Q000821:virology; D006224:Cricetinae; D003840:Deoxycholic Acid / Q000494:pharmacology; D004669:Encephalitis Viruses, Tick-Borne / Q000187:drug effects / Q000276:immunology* / Q000472:pathogenicity; D006358:Hot Temperature; D008253:Macaca mulatta; D008647:Mesocricetus; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D012692:Serial Passage; D014765:Viral Vaccines; D014774:Virulence
chemical_list: D014765:Viral Vaccines; D003840:Deoxycholic Acid
vernacular_title: Analiz stabil'nosti nekotorykh svoĭstv virusa kleshchevogo éntsefalita (shtamm 205) pri passirovanii na myshakh.