Cloning and expression of a second human natural killer cell granule tryptase, HNK-Tryp-2/granzyme 3.
Cytotoxic lymphocytes possess a number of serine proteases (granzymes) usually localized in cytoplasmic granules. To date, the DNA sequences of four human granzymes have been reported. A fifth human granzyme (granzyme 3) has been biochemically purified and its N-terminal amino acid sequence has been reported. This enzyme was described as possessing tryptase activity, cleaving synthetic substrates after arginine or lysine. We recently cloned a rat granzyme tryptase (RNK-Tryp-2), and used this cDNA to screen human cDNA libraries. Isolation of cDNA fragments of a human gene could be overlapped to provide a complete cDNA sequence, which we designated HNK-Tryp-2. The N-terminal amino acid sequence deduced from HNK-Tryp-2 was identical to that reported for granzyme 3. This gene appears to be a single copy gene that is expressed in isolated natural killer cells and T cells as well as in tissues containing these cells.
Sayers
T J
TJ
Biological Carcinogenesis and Developmental Program, SAIC Frederick, National Cancer Institute, Frederick Cancer Research and Developmental Center, Maryland 21702, USA.
Lloyd
A R
AR
McVicar
D W
DW
O'Connor
M D
MD
Kelly
J M
JM
Carter
C R
CR
Wiltrout
T A
TA
Wiltrout
R H
RH
Smyth
M J
MJ
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000302:isolation & purification; D053804:Granzymes; D006801:Humans; D007694:Killer Cells, Natural / Q000201:enzymology*; D051379:Mice; D008969:Molecular Sequence Data; D051381:Rats; D012697:Serine Endopeptidases / Q000737:chemistry / Q000235:genetics*
D018076:DNA, Complementary; C088546:GZMK protein, human; D053804:Granzymes; D012697:Serine Endopeptidases