Effects of a synthetic N-terminal fragment of stanniocalcin on the metabolism of mammalian bone in vitro.
A synthetic peptide corresponding to the N-terminal amino acid residues of stanniocalcin (STC1-20) and including a region that is known to be an active site in teleosts was prepared and tested for its effects on the metabolism of mammalian bone in vitro. STC1-20 (10(-10)-10(-12) M) inhibited increases in the number of tartrate-resistant acid phosphatase-positive, multinucleated cells promoted by an N-terminal fragment of human parathyroid hormone (hPTH1-34) in cultures of murine hemopoietic cells. STC1-20 also slightly decreased the rate of loss of radioactivity from calvariae of fetal rats that had been prelabeled with 45Ca, both with and without stimulation by hPTH1-34. The accumulation of cAMP induced by hPTH1-34 in ROS 17/2.8-5 cells was suppressed by STC1-20 (10(-10)-10(-12) M). Treatment with STC1-20 (10(-11)-10(-13) M) caused increases of the rate of incorporation of [3H]proline into the collagenase-digestible protein of calvariae in newborn mice. From these results, it appears that STC1-20 has diverse effects on the metabolism of mammalian bone, causing a biphasic response. Such effects have not been observed with intact stanniocalcin or with materials from the corpuscles of Stannius and they are also different from the effects of hPTH1-34.
Yoshiko
Y
Y
Pharmaceutical Research Laboratories, POLA R and D Laboratories, Yokohama, Japan.
Kosugi
T
T
Koide
Y
Y
D000135:Acid Phosphatase / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D001842:Bone and Bones / Q000187:drug effects / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D002452:Cell Count / Q000187:drug effects; D000242:Cyclic AMP / Q000378:metabolism; D005260:Female; D006023:Glycoproteins / Q000737:chemistry / Q000494:pharmacology*; D006728:Hormones / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D010281:Parathyroid Hormone / Q000494:pharmacology; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012474:Salmon; D017386:Sequence Homology, Amino Acid; D012886:Skull; D000071681:Tartrate-Resistant Acid Phosphatase; D019379:Teriparatide; D014407:Tumor Cells, Cultured
D006023:Glycoproteins; D006728:Hormones; D007527:Isoenzymes; D010281:Parathyroid Hormone; D010446:Peptide Fragments; D019379:Teriparatide; C051795:teleocalcin; D000242:Cyclic AMP; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase; D002118:Calcium