Do intestinal parasites interfere with the seroepidemiologic surveillance of Schistosoma mansoni infection?
In view of the known cross-reactivity of sera from patients with intestinal parasites to some Schistosoma mansoni antigens, field work was conducted in an area of Venezuela non-endemic for schistosomiasis using the routine immunoenzymatic assay (ELISA) with soluble egg antigen (SEA). False positive reactions represented 15.3% of the total population as determined by SEA-ELISA. SEA-immunoblotting of the false positive sera indicated that protein fractions of 91 and 80 kDa appear to be responsible for cross-reactivity. Sera from hookworm infected individuals produced a higher frequency and intensity of cross-reaction than other sera. SEA-fractions of 105, 54, 46, 42, 32, 25 and 15 kDa were the most specific.
Alarcón de Noya
B
B
Sección de Biohelmintiasis, Universidad Central de Venezuela, Caracas, Venezuela.
Colmenares
C
C
Losada
S
S
Fermin
Z
Z
Masroua
G
G
Ruiz
L
L
Soto
L
L
Noya
O
O
D000293:Adolescent; D000328:Adult; D000818:Animals; D000947:Antigens, Helminth / Q000302:isolation & purification; D002648:Child; D002675:Child, Preschool; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D005189:False Positive Reactions; D006725:Hookworm Infections / Q000150:complications / Q000453:epidemiology*; D006801:Humans; D007223:Infant; D008875:Middle Aged; D015995:Prevalence; D015203:Reproducibility of Results; D012550:Schistosoma mansoni / Q000302:isolation & purification; D012555:Schistosomiasis mansoni / Q000150:complications / Q000453:epidemiology*; D016036:Seroepidemiologic Studies; D014687:Venezuela / Q000453:epidemiology
D000947:Antigens, Helminth