title: Identification of a second functional glutaredoxin encoded by the bacteriophage T4 genome.
abstract: Thioredoxins and glutaredoxins are small ubiquitous redox proteins that were discovered as hydrogen donors for ribonucleotide reductase, the key enzyme for deoxyribonucleotide biosynthesis. Some organisms encode more than one redox protein. In this study, we demonstrate that an open reading frame in the bacteriophage T4 genome, reported earlier and designated as Y55.7 (Tomaschewski, J., and Rüger, W. (1987) Nucleic Acids Res. 15, 3632-3633), encodes a second functional redox protein. Gene y55.7 was cloned and expressed in Escherichia coli. Purified Y55.7 protein had glutathione-dependent thioltransferase and dehydroascorbate reductase activities indicative of a functional glutaredoxin. The protein is expressed at all stages of the T4 infection cycle and can serve as a hydrogen donor for the phage ribonucleotide reductase in in vitro experiments.
authors.affiliation: Department of Biochemistry and Biophysics, Oregon State University, Corvallis, Oregon 97331-7305, USA.
authors.forename: B O
authors.initials: BO
authors.lastname: Gvakharia
authors.forename: E
authors.initials: E
authors.lastname: Hanson
authors.forename: E K
authors.initials: EK
authors.lastname: Koonin
authors.forename: C K
authors.initials: CK
authors.lastname: Mathews
mesh_terms: D017122:Bacteriophage T4 / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D017931:DNA Primers / Q000737:chemistry; D005814:Genes, Viral; D054477:Glutaredoxins; D005978:Glutathione / Q000378:metabolism; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D010088:Oxidoreductases; D011233:Precipitin Tests; D017433:Protein Structure, Secondary; D011506:Proteins / Q000378:metabolism*; D015678:Viral Structural Proteins / Q000235:genetics
chemical_list: D017931:DNA Primers; D054477:Glutaredoxins; D011506:Proteins; D015678:Viral Structural Proteins; D010088:Oxidoreductases; D005978:Glutathione