Generation and characterization of circular Bacillus subtilis RNase P RNA; activation by RNase P protein.
A circular form of Bacillus subtilis ribonuclease P RNA (C-P RNA) was generated in vitro by splicing permuted intron-exon (PIE) sequences containing the P RNA sequence. Steady-state cleavage of pre-tRNA(Asp) catalyzed by circular P RNA is slightly faster than the linear form. Furthermore, steady-state turnover catalyzed by circular RNase P RNA is activated by the addition of the Bacillus subtilis protein component of RNase P, to a rate constant equal to the linear holoenzyme under identical conditions. Also, the circles are resistant to nuclease degradation, have less sequence heterogeneity, and may enhance the formation of a unique structure. Therefore, circular forms of RNase P RNA should prove useful for mutagenesis and structural studies.
Puttaraju
M
M
Department of Biochemistry, Duke University Medical Center, Durham, NC 27710, USA.
Beebe
J A
JA
Niranjanakumari
S
S
Been
M D
MD
Fierke
C A
CA
D001412:Bacillus subtilis / Q000378:metabolism*; D004355:Drug Stability; D004722:Endoribonucleases / Q000378:metabolism*; D007700:Kinetics; D015394:Molecular Structure; D012313:RNA / Q000737:chemistry / Q000378:metabolism*; D012322:RNA Precursors / Q000378:metabolism; D012326:RNA Splicing; D012329:RNA, Bacterial / Q000737:chemistry / Q000378:metabolism*; D016337:RNA, Catalytic / Q000378:metabolism*; D000079962:RNA, Circular; D012349:RNA, Transfer, Asp / Q000378:metabolism; D043262:Ribonuclease P
D012322:RNA Precursors; D012329:RNA, Bacterial; D016337:RNA, Catalytic; D000079962:RNA, Circular; D012349:RNA, Transfer, Asp; D012313:RNA; D004722:Endoribonucleases; D043262:Ribonuclease P