[Analysis of copies of the suffix-retroposon of Drosophila, produced using PCR on genomic DNA].
Nineteen DNA clones, containing copies of the suffix element retroposon of the Drosophila genome and produced by a polymerase chain reaction (PCR), were sequenced. Insertions of the copies in both orientations into microsatellite sequences (CAACA)n/(TGTTG)n and (TTTGT)n/(CACAAA)n were revealed. It was found that, if the microsatellite sequence has both a decanucleotide GCGGCCCGGG (GC-box) and a colinear alternating sequence (A)5(T)4(A)3(T)2(A)1(t)n (AT-box), the insertion of the suffix occurs in only one orientation. It is suggested, that in such apparently heterochromatic sequences, suffix element copies and probably some other retroposons can be inserted a particular orientation by means of site-specific recombination. An approach to analysis of the molecular organization of such sequences is proposed.
Kolesnikov
A V
AV
Ponomarenko
N A
NA
Churikov
N A
NA
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004330:Drosophila / Q000235:genetics*; D016678:Genome; D006570:Heterochromatin; D017403:In Situ Hybridization; D018895:Microsatellite Repeats; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D018626:Retroelements / Q000235:genetics*
D006570:Heterochromatin; D018626:Retroelements
Analiz kopiĭ suffiksa-retropozona drozofily, narobotannykh s pomoshch'iu PTsR na genomnoĭ DNK.