Molecular modeling of the amino-terminal zinc ring domain of BRCA1.
The equine herpes virus zinc ring domain nuclear magnetic resonance structure was used for homology-based modeling of the amino-terminal zinc ring domain of the BRCA1 breast and ovarian cancer susceptibility gene. The zinc ring domain of BRCA1 is of particular interest because it is the location of significant and frequently occurring missense (Cys(61)Gly, Cys(64)Gly, and Cys(64)Tyr) and frameshift (185delAG) mutations observed in several high-risk kindreds. The BRCA1 zinc ring domain possesses 54% sequence similarity with the equine herpes virus zinc ring domain. The model structure undergoes little conformational variance after 140 ps of solvated molecular dynamics. This model proposes BRCA1 zinc ring domain residues that may play a role in DNA binding and/or protein-protein interactions. These predictions provide a point of departure for the design of mutants to probe BRCA1 zinc ring domain functionality.
Bienstock
R J
RJ
National Institute of Environmental Health Sciences, Laboratories of Quantitative and Computational Biology, Research Triangle Park, North Carolina 27709, USA. rachelle@picard.cichs.nih.gov
Darden
T
T
Wiseman
R
R
Pedersen
L
L
Barrett
J C
JC
D000595:Amino Acid Sequence; D019313:BRCA1 Protein; D003198:Computer Simulation; D004268:DNA-Binding Proteins / Q000737:chemistry*; D006564:Herpesviridae / Q000737:chemistry; D008667:Metalloproteins / Q000737:chemistry; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000737:chemistry*; D009687:Nuclear Proteins; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D012997:Solvents; D014157:Transcription Factors / Q000737:chemistry*; D025521:Tumor Suppressor Proteins; D014764:Viral Proteins / Q000737:chemistry; D015032:Zinc
D019313:BRCA1 Protein; D004268:DNA-Binding Proteins; D008667:Metalloproteins; D009363:Neoplasm Proteins; D009687:Nuclear Proteins; D012997:Solvents; D014157:Transcription Factors; D025521:Tumor Suppressor Proteins; D014764:Viral Proteins; D015032:Zinc