Molecular epidemiology of an SHV-5 extended-spectrum beta-lactamase in enterobacteriaceae isolated from infants in a neonatal intensive care unit.
Klebsiella oxytoca that produced extended-spectrum beta-lactamase (ESBL) and were resistant to ceftazidime were isolated from infants in a neonatal intensive care unit (NICU). During a 30-week period, 3 infants developed infections and an additional 60 infants were colonized with these bacteria. The molecular typing data suggested transmission of a single strain of ceftazidime-resistant K. oxytoca among 48 of the 63 infants. The ESBL of 46 of the 48 similar isolates, 14 of the remaining 15 isolates, and 6 other Enterobacteriaceae appeared to be associated with a conjugative plasmid of approximately 85 kb. The ESBL gene was cloned, and DNA sequencing confirmed that the ESBL was an SHV-5. Hybridization data suggested that the SHV-5 gene was transmitted to other Enterobacteriaceae in vivo. The spread of the ESBL was reduced through adherence to infection control practices.
Venezia
R A
RA
Department of Pathology and Laboratory Medicine, Albany Medical Center Hospital, New York 12208, USA.
Scarano
F J
FJ
Preston
K E
KE
Steele
L M
LM
Root
T P
TP
Limberger
R
R
Archinal
W
W
Kacica
M A
MA
D015373:Bacterial Typing Techniques; D002442:Ceftazidime / Q000627:therapeutic use; D018550:Cephalosporin Resistance / Q000235:genetics; D002511:Cephalosporins / Q000627:therapeutic use; D003001:Cloning, Molecular; D003428:Cross Infection / Q000188:drug therapy / Q000453:epidemiology* / Q000382:microbiology; D004196:Disease Outbreaks; D017809:Fatal Outcome; D005798:Genes, Bacterial; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D015931:Intensive Care, Neonatal; D007525:Isoelectric Focusing; D007709:Klebsiella / Q000201:enzymology* / Q000302:isolation & purification; D007710:Klebsiella Infections / Q000188:drug therapy / Q000453:epidemiology* / Q000382:microbiology; D010957:Plasmids; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism*
D002511:Cephalosporins; D002442:Ceftazidime; D001618:beta-Lactamases