Structure and expression of the rat mRNA encoding a novel member of the fibroblast growth factor family.
We isolated the cDNA encoding a novel member of the fibroblast growth factor (FGF) family from rat embryos by homology-based polymerase chain reaction. The FGF-related cDNA encodes a protein of 215 amino acids (approximately 24 kDa), which has a conserved approximately 120-amino acid core with approximately 30-60% amino acid sequence identity with the FGF family. This protein with a hydrophobic amino terminus appears to be a secreted protein. The cDNA was translated in a coupled in vitro transcription-translation system. The molecular mass of the translation product was observed to be approximately 26 kDa. The expression of the FGF-related mRNA in the rat embryo and adult tissues was determined by Northern analysis and in situ hybridization. The mRNA was expressed in several discrete regions of the embryo. In adult tissues, the mRNA was preferentially expressed in the lung. The expression profile of the FGF-related mRNA was different from those of other FGF family mRNAs. As this protein is the 10th documented protein related to FGFs, we tentatively term this protein FGF-10.
Yamasaki
M
M
Department of Genetic Biochemistry, Kyoto University Faculty of Pharmaceutical Sciences, Kyoto 606-01, Japan.
Miyake
A
A
Tagashira
S
S
Itoh
N
N
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017124:Conserved Sequence; D018076:DNA, Complementary; D004622:Embryo, Mammalian; D051526:Fibroblast Growth Factor 10; D051518:Fibroblast Growth Factor 3; D051523:Fibroblast Growth Factor 7; D005346:Fibroblast Growth Factors / Q000096:biosynthesis* / Q000737:chemistry; D005865:Gestational Age; D006133:Growth Substances / Q000737:chemistry; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D009928:Organ Specificity; D016133:Polymerase Chain Reaction; D011518:Proto-Oncogene Proteins / Q000737:chemistry; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; C496465:Fgf10 protein, rat; C489199:Fgf3 protein, rat; C496455:Fgf7 protein, rat; D051526:Fibroblast Growth Factor 10; D051518:Fibroblast Growth Factor 3; D006133:Growth Substances; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D051523:Fibroblast Growth Factor 7; D005346:Fibroblast Growth Factors