A new allogeneic model for metastatic melanoma.
Metastatic melanoma cells, clonally derived from an affected lymph node of an ultraviolet-irradiated laboratory opossum, were allografted subcutaneously into suckling young, juveniles and adults to determine their tumorigenicity and metastatic potential. All injected 1- and 3-week-old suckling young survived well beyond weaning at 8 weeks. One died 12 weeks after injection from the effects of rampant metastatic involvement, while the rest were killed 13 to 26 weeks after injection. At necropsy, most animals showed extensive primary tumour growth, many showed metastasis to nodes and/or lungs, and in some there was dissemination to distant sites including liver and spleen. Animals injected as juveniles or adults rejected the allografts. Injection of allogeneic malignant melanoma cells during early postnatal development facilitates successful, long-term allografting and metastasis without concomitant immunosuppressive agents. Developmental lack of self-recognition (immunological immaturity) or induced tolerance may be responsible. This unique model system will be useful for further metastasis studies and may be valuable for investigations of novel antineoplastic therapies.
Robinson
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Department of Genetics, Southwest Foundation for Biomedical Research, San Antonio, Texas 78228-0147, USA.
Dooley
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D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D008297:Male; D008545:Melanoma / Q000473:pathology* / Q000556:secondary* / Q000648:ultrastructure; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000473:pathology / Q000648:ultrastructure; D009381:Neoplasms, Radiation-Induced / Q000473:pathology / Q000556:secondary / Q000648:ultrastructure; D009893:Opossums; D014184:Transplantation, Homologous