title: Inverse polymerase chain reaction for cloning complete human immunoglobulin variable regions and leaders conserving the original sequence.
authors.affiliation: Research Laboratory of Biotechnology, University Hospital-I.R.C.C.S. Policlinico S. Matteo, P. le Golgi 2, Pavia, Italy.
authors.forename: V
authors.initials: V
authors.lastname: Perfetti
authors.forename: M
authors.initials: M
authors.lastname: Sassano
authors.forename: P
authors.initials: P
authors.lastname: Ubbiali
authors.forename: M C
authors.initials: MC
authors.lastname: Vignarelli
authors.forename: E
authors.initials: E
authors.lastname: Arbustini
authors.forename: A
authors.initials: A
authors.lastname: Corti
authors.forename: G
authors.initials: G
authors.lastname: Merlini
mesh_terms: D000686:Amyloidosis / Q000276:immunology; D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods*; D017124:Conserved Sequence; D017931:DNA Primers; D004587:Electrophoresis, Agar Gel; D006801:Humans; D007135:Immunoglobulin Variable Region / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D017931:DNA Primers; D007135:Immunoglobulin Variable Region