Studies on interaction of different zinc-finger domains of Xenopus laevis TFIIIA with eukaryotic 5S rRNAs.
We studied interactions between various domains of transcriptional factor IIIA (TFIIIA) from Xenopus laevis containing variable number of zinc finger with 5S ribosomal RNA (5S rRNA) from plants. The results clearly show that the complexes of peptide-RNA are formed in addition they strongly support that the tertiary structure of 5S rRNA is well conserved among eukaryotes. We optimalized conditions of complex formation reaction in order to study topography of these complexes.
Barciszewska
M Z
MZ
Institute of Biorganic Chemistry, Polish Academy of Sciences, Poznan, Poland.
Giel-Pietraszuk
M
M
Thogersen
H C
HC
Barciszewski
J
J
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D018749:RNA, Plant / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012341:RNA, Ribosomal, 5S / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D035582:Transcription Factor TFIIIA; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014982:Xenopus laevis; D016335:Zinc Fingers
D004268:DNA-Binding Proteins; D018749:RNA, Plant; D012341:RNA, Ribosomal, 5S; D011994:Recombinant Proteins; D035582:Transcription Factor TFIIIA; D014157:Transcription Factors