The mariner transposable element in natural populations of Drosophila teissieri.
The mariner transposable elements of several natural populations of Drosophila teissieri, a rainforest species endemic to tropical Africa, were studied. Natural populations trapped along a transect from Zimbabwe to the Ivory Coast were analyzed by Southern blotting, in situ hybridization, cloning, and sequencing of PCR products. The Brazzaville population had some full-length elements, while the remaining populations had mainly deleted elements. The main class of deleted elements lacked a 500-bp segment. A mechanism is proposed that could generate such elements rapidly. In situ hybridizations showed that there are no mariner elements in pericentromeric heterochromatin. Finally, the phylogeny of the Mos1-like mariner full-length elements is consistent with vertical transmission from the ancestor of the melanogaster subgroup.
Brunet
F
F
Laboratoire Populations, Génétique et Evolution, CNRS, 91198 Gif sur Yvette, Cedex, France.
Godin
F
F
Bazin
C
C
David
J R
JR
Capy
P
P
D000349:Africa; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D004251:DNA Transposable Elements / Q000235:genetics; D004330:Drosophila / Q000145:classification / Q000235:genetics*; D005260:Female; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
D004251:DNA Transposable Elements