title: Two separate functions are encoded by the carboxyl-terminal domains of the yeast cyclase-associated protein and its mammalian homologs. Dimerization and actin binding.
abstract: The yeast adenylyl cyclase-associated protein, CAP, was identified as a component of the RAS-activated cyclase complex. CAP consists of two functional domains separated by a proline-rich region. One domain, which localizes to the amino terminus, mediates RAS signaling through adenylyl cyclase, while a domain at the carboxyl terminus is involved in the regulation of cell growth and morphogenesis. Recently, the carboxyl terminus of yeast CAP was shown to sequester actin, but whether this function has been conserved, and is the sole function of this domain, is unclear. Here, we demonstrate that the carboxyl-terminal domains of CAP and CAP homologs have two separate functions. We show that carboxyl-terminals of both yeast CAP and a mammalian CAP homolog, MCH1, bind to actin. We also show that this domain contains a signal for dimerization, allowing both CAP and MCH1 to form homodimers and heterodimers. The properties of actin binding and dimerization are mediated by separate regions on the carboxyl terminus; the last 27 amino acids of CAP being critical for actin binding. Finally, we present evidence that links a segment of the proline-rich region of CAP to its localization in yeast. Together, these results suggest that all three domains of CAP proteins are functional.
authors.affiliation: Department of Cell Biology and Anatomy, Mount Sinai School of Medicine, New York, New York 10029, USA.
authors.forename: A
authors.initials: A
authors.lastname: Zelicof
authors.forename: V
authors.initials: V
authors.lastname: Protopopov
authors.forename: D
authors.initials: D
authors.lastname: David
authors.forename: X Y
authors.initials: XY
authors.lastname: Lin
authors.forename: V
authors.initials: V
authors.lastname: Lustgarten
authors.forename: J E
authors.initials: JE
authors.lastname: Gerst
mesh_terms: D000199:Actins / Q000378:metabolism*; D000262:Adenylyl Cyclases / Q000737:chemistry; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002451:Cell Compartmentation; D018797:Cell Cycle Proteins; D003598:Cytoskeletal Proteins; D029721:Drosophila Proteins; D005347:Fibroblasts; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D008840:Microfilament Proteins; D008969:Molecular Sequence Data; D011233:Precipitin Tests; D011392:Proline; D011485:Protein Binding; D011487:Protein Conformation; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D017385:Sequence Homology; D013329:Structure-Activity Relationship; D015003:Yeasts
chemical_list: D000199:Actins; C081782:Cap1 protein, rat; C410502:Capt protein, Drosophila; D018797:Cell Cycle Proteins; D003598:Cytoskeletal Proteins; D029721:Drosophila Proteins; D005656:Fungal Proteins; D008840:Microfilament Proteins; D011506:Proteins; D011993:Recombinant Fusion Proteins; D011392:Proline; D000262:Adenylyl Cyclases