title: Human high affinity, Na(+)-dependent L-glutamate/L-aspartate transporter GLAST-1 (EAAT-1): gene structure and localization to chromosome 5p11-p12.
abstract: The gene of the human L-glutamate transporter hGLAST-1 (EAAT-1) has been isolated and characterized. The 1626 bp cDNA open reading frame (542 aa) is distributed over ten exons and at least 85 kb on chromosome 5p11-p12. The gene is unrelated to any other previously described neurotransmitter transporter gene family, but its exon/intron structure corresponds largely to that of the Na(+)-dependent neutral amino acid transporter ASCT-1. GLAST-1, ASCT-1 and the glutamate transporters GLT-1 and EAAC-1 have strongly similar amino acid sequences. The L-glutamate transporter gene structures might help to understand the correlation of L-glutamate reuptake in neurodegenerative disorders.
authors.affiliation: Institut für Biochemie, Universität zu Köln, Germany. akc03@rs1.rrz.uni-kocln.de
authors.forename: W
authors.initials: W
authors.lastname: Stoffel
authors.forename: J
authors.initials: J
authors.lastname: Sasse
authors.forename: M
authors.initials: M
authors.lastname: Düker
authors.forename: R
authors.initials: R
authors.lastname: Müller
authors.forename: K
authors.initials: K
authors.lastname: Hofmann
authors.forename: T
authors.initials: T
authors.lastname: Fink
authors.forename: P
authors.initials: P
authors.lastname: Lichter
mesh_terms: D000595:Amino Acid Sequence; D027322:Amino Acid Transport System X-AG; D001483:Base Sequence; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics*; D002874:Chromosome Mapping; D002895:Chromosomes, Human, Pair 5; D003001:Cloning, Molecular; D004247:DNA; D006023:Glycoproteins / Q000737:chemistry / Q000235:genetics*; D006801:Humans; D007438:Introns; D008969:Molecular Sequence Data; D012964:Sodium / Q000494:pharmacology*
chemical_list: D027322:Amino Acid Transport System X-AG; D002352:Carrier Proteins; D006023:Glycoproteins; D004247:DNA; D012964:Sodium