title: Cloning and chromosomal mapping of three novel genes, GPR9, GPR10, and GPR14, encoding receptors related to interleukin 8, neuropeptide Y, and somatostatin receptors.
abstract: We employed the polymerase chain reaction and genomic DNA library screening to clone novel human genes, GPR9 and GPR10, and a rat gene, GPR14. GPR9, GPR10, and GPR14 each encode G protein-coupled receptors. GPR10 and GPR14 are intronless within their coding regions, while GPR9 contains at least one intron. The receptor encoded by GPR9 shares the highest identity with human IL-8 receptor type B (38% overall and 53% in the transmembrane regions), followed by IL-8 receptor type A (36% overall and 51% in the transmembrane domains). GPR10 encodes a receptor that shares highest identity with the neuropeptide Y receptor (31% overall and 46% in the transmembrane domains). The receptor encoded by GPR14 shares highest identity with the somatostatin receptor SSTR 4 (27% overall and 41% in the transmembrane domains). Fluorescence in situ hybridization analysis localized GPR9 to chromosome 8p11.2-p12 and GPR10 to chromosome 10q25.3-q26.
authors.affiliation: Addiction Research Foundation, Toronto, Ontario, Canada.
authors.forename: A
authors.initials: A
authors.lastname: Marchese
authors.forename: M
authors.initials: M
authors.lastname: Heiber
authors.forename: T
authors.initials: T
authors.lastname: Nguyen
authors.forename: H H
authors.initials: HH
authors.lastname: Heng
authors.forename: V R
authors.initials: VR
authors.lastname: Saldivia
authors.forename: R
authors.initials: R
authors.lastname: Cheng
authors.forename: P M
authors.initials: PM
authors.lastname: Murphy
authors.forename: L C
authors.initials: LC
authors.lastname: Tsui
authors.forename: X
authors.initials: X
authors.lastname: Shi
authors.forename: P
authors.initials: P
authors.lastname: Gregor
mesh_terms: D000595:Amino Acid Sequence; D015703:Antigens, CD / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D015152:Blotting, Northern; D001921:Brain / Q000378:metabolism; D002874:Chromosome Mapping; D002898:Chromosomes, Human, Pair 8; D003001:Cloning, Molecular; D004247:DNA; D015723:Gene Library; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D016209:Interleukin-8 / Q000378:metabolism; D008214:Lymphocytes / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000302:isolation & purification / Q000378:metabolism; D018123:Receptors, Interleukin / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D023062:Receptors, Interleukin-8A; D017476:Receptors, Neuropeptide Y / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D011957:Receptors, Opioid / Q000737:chemistry / Q000235:genetics; D017481:Receptors, Somatostatin / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry; D017386:Sequence Homology, Amino Acid
chemical_list: D015703:Antigens, CD; D016209:Interleukin-8; D012333:RNA, Messenger; D018123:Receptors, Interleukin; D023062:Receptors, Interleukin-8A; D017476:Receptors, Neuropeptide Y; D011957:Receptors, Opioid; D017481:Receptors, Somatostatin; D011994:Recombinant Proteins; D004247:DNA