A comparative immunocytochemical study using an antiserum against a synthetic analogue of the corpora cardiaca peptide Pea-CAH-I (MI, neurohormone D) of Periplaneta americana.
An antiserum against the octapeptide Pea-CAH-I, a member of the adipokinetic hormone/red pigment-concentrating hormone family, has been produced for immunocytochemical staining in insects and various other invertebrate species. The anti-Pea-CAH-I serum stains the glandular corpora cardiaca cells of those insect species that synthesize identical or structurally similar peptides. In the corpora cardiaca of species producing peptides with a different C-terminus, these cells remain unstained. Pea-CAH-I-like immunoreactivity has also been found in neurons of the central nervous system of all invertebrate orders studied. The antiserum recognizes the C-terminal sequence Pro-Asn-Trp-NH2 of the Pea-CAH-I molecule as established by enzyme immunoassay. The widespread Pea-CAH-I-like immunoreactivity in all nervous systems of the studied animals probably does not reflect the presence of Pea-CAH-I but the occurrence of peptides carrying similar epitopes.
Eckert
M
M
Institut für Allgemeine Zoologie und Tierphysiologie, F.-Schiller-Universität, Jena, Germany.
Gabriel
J
J
Birkenbeil
H
H
Greiner
G
G
Rapus
J
J
Gäde
G
G
D000595:Amino Acid Sequence; D000818:Animals; D000918:Antibody Specificity; D001923:Brain Chemistry; D005260:Female; D017952:Ganglia, Invertebrate / Q000737:chemistry; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D007301:Insect Hormones / Q000032:analysis / Q000138:chemical synthesis / Q000276:immunology; D007313:Insecta; D008297:Male; D016253:Microscopy, Immunoelectron; D008969:Molecular Sequence Data; D009420:Nervous System / Q000737:chemistry / Q000648:ultrastructure; D009479:Neuropeptides / Q000032:analysis / Q000138:chemical synthesis / Q000276:immunology*; D010526:Periplaneta / Q000737:chemistry*; D011817:Rabbits
D007301:Insect Hormones; D009479:Neuropeptides; C043192:glutamyl-valyl-asparaginyl-phenylalanyl-seryl-prolyl-asparaginyl-tryptophanamide