cis-regulatory elements of the peroxidase gene in Arabidopsis thaliana involved in root-specific expression and responsiveness to high-salt stress.
The pattern of organ-specific expression of Arabidopsis thaliana peroxidase was similar to that in horseradish. Tobacco plants transformed with the gus gene fused to the -580 bp deletion (Ea-580) of A. thaliana exhibited high GUS expression in roots. Gel retardation and footprinting analyses showed that at least three domains of fragment between -172 and -1 bp have cis-acting element activities. Several physiological functions for plant peroxidases have been suggested; for example, a metabolic adaptation to salinity in the environment can be induced by certain specific elements of the peroxidase gene. The prxEa promoter fragments (Ea-580 and Ea-390) show multiple cis-acting elements in the control of expression in high-salt stress. These data suggest that the DNA-binding factor may be involved in the regulation of gene expression in specific organ and salt stress.
Wanapu
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Department of Biochemistry, Faculty of Science, Prince of Songkla University, Hatyai, Thailand. ichokcha@ratree.psu.ac.th
Shinmyo
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A
D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics*; D001483:Base Sequence; D018983:DNA Footprinting; D015870:Gene Expression; D017343:Genes, Plant; D005966:Glucuronidase / Q000096:biosynthesis; D006735:Horseradish Peroxidase / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D007700:Kinetics; D008969:Molecular Sequence Data; D009994:Osmolar Concentration; D010544:Peroxidases / Q000096:biosynthesis* / Q000235:genetics* / Q000378:metabolism; D018517:Plant Roots; D030821:Plants, Genetically Modified; D010947:Plants, Toxic; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D012045:Regulatory Sequences, Nucleic Acid; D017384:Sequence Deletion; D014026:Nicotiana
D011993:Recombinant Fusion Proteins; D006735:Horseradish Peroxidase; D010544:Peroxidases; D005966:Glucuronidase