Cytotoxicity of antitumor agents toward cultured murine leukemia L1210 cells under a serum-deprived apoptotic condition.
Apoptosis is known to be induced in L1210 cells that are cultured in serum-deprived medium. In this study, inhibitory effects of some antitumor agents on the growth of L1210 cells cultured in a normal and a serum-deprived medium were compared. All the antitumor agents examined were equally cytotoxic to cells in both media, indicating that the cytotoxic effects of these agents were not coupled with programmed apoptosis so as to induce synergistic cell death. The single cell-gel electrophoresis assay (comet assay) revealed that antitumor agents induced DNA strand breakage in a dose-dependent manner under either normal or serum-deprived culture conditions without any synergistic increases in DNA strand breakage.
Kasamatsu
T
T
Faculty of Pharmaceutical Sciences, Nagoya City University, Japan.
Kohda
K
K
Kawazoe
Y
Y
D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology; D000964:Antimetabolites, Antineoplastic / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000145:classification / Q000494:pharmacology*; D018906:Antineoplastic Agents, Alkylating / Q000494:pharmacology; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D002945:Cisplatin / Q000494:pharmacology; D016895:Culture Media, Serum-Free / Q000494:pharmacology*; D004249:DNA Damage; D004273:DNA, Neoplasm / Q000032:analysis; D004587:Electrophoresis, Agar Gel; D007939:Leukemia L1210 / Q000473:pathology*; D051379:Mice; D014407:Tumor Cells, Cultured / Q000187:drug effects
D000903:Antibiotics, Antineoplastic; D000964:Antimetabolites, Antineoplastic; D000970:Antineoplastic Agents; D018906:Antineoplastic Agents, Alkylating; D000972:Antineoplastic Agents, Phytogenic; D016895:Culture Media, Serum-Free; D004273:DNA, Neoplasm; D002945:Cisplatin