The human S3a ribosomal protein: sequence, location and cell-free transcription of the functional gene.
The intron-containing gene encoding human ribosomal protein S3a (hRPS3a) was isolated by utilizing a PCR-based strategy to detect a gene-specific intron which was subsequently used as a probe for cloning of the entire gene. The hRPS3a gene is composed of six exons and five introns spanning 5013 bp. As described for other hRP-encoding genes, the promoter lacks a canonical TATA sequence and a defined CAAT box. Primer extension experiments, as well as cell-free transcription, revealed that a cytosine functions as the major transcription start point in a polypyrimidine region, but a guanosine at position -1 was also able to initiate transcription. Hybridization analysis of chromosomal DNA from a panel of human-rodent somatic cell hybrids revealed that hRPS3a is encoded by a single locus in the human genome, present on chromosome 4.
Nolte
D
D
Institut für Molekularbiologie und Tumorforschung, Philipps-Universität Marburg, Germany.
Taimor
G
G
Kalff-Suske
M
M
Seifart
K H
KH
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002474:Cell-Free System; D002874:Chromosome Mapping; D006224:Cricetinae; D004247:DNA / Q000235:genetics; D018628:Gene Dosage; D015870:Gene Expression; D006367:HeLa Cells; D006801:Humans; D006822:Hybrid Cells; D007438:Introns / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012269:Ribosomal Proteins / Q000737:chemistry / Q000235:genetics*; D014158:Transcription, Genetic / Q000235:genetics*
C031016:RPS3A protein, human; D012269:Ribosomal Proteins; C488930:Rsp3a1 protein, mouse; D004247:DNA