Structure and characterization of the gene encoding a mouse kappa3-related opioid receptor.
A genomic clone comprising the entire cDNA sequence encoding a mouse kappa3-related opioid receptor (KOR-3) was isolated by screening a mouse genomic library with a radiolabeled mouse KOR-3 cDNA probe. Sequence analysis of the genomic clone indicates that the KOR-3 gene contains five exons separated by four introns. The transcription start point (tsp) of KOR-3 was mapped by primer extension analysis of RNAs synthesized either in vivo or in vitro. A TATA-box and several potential regulatory elements, including five GRE sites, four NF-E1 binding sites and one MRE site, are present in the 2 kb of 5'-flanking region. A putative poly(A) signal (AATAAA) is found in the 3'-flanking region.
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Cotzias Laboratory of Neuro-Oncology, Memorial Sloan-Kettering Cancer Center, New York, NY 10021, USA.
Xu
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Pasternak
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GW
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000378:metabolism; D050981:Erythroid-Specific DNA-Binding Factors; D006367:HeLa Cells; D006801:Humans; D051379:Mice / Q000235:genetics*; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics* / Q000378:metabolism; D011965:Receptors, Glucocorticoid / Q000378:metabolism; D011957:Receptors, Opioid; D017422:Sequence Analysis, DNA; D016385:TATA Box; D014157:Transcription Factors / Q000378:metabolism; D014158:Transcription, Genetic; D000094942:Nociceptin Receptor
D004268:DNA-Binding Proteins; D050981:Erythroid-Specific DNA-Binding Factors; D009419:Nerve Tissue Proteins; D011965:Receptors, Glucocorticoid; D011957:Receptors, Opioid; D014157:Transcription Factors; D000094942:Nociceptin Receptor