Di-isodityrosine, a novel tetrametric derivative of tyrosine in plant cell wall proteins: a new potential cross-link.
A novel amino acid, di-isodityrosine, has been isolated from hydrolysates of cell walls of tomato cell culture. Analysis by UV spectrometry, partial derivatization with 2,4-dinitrofluorobenzene and mass and NMR spectrometry show that the compound is composed to two molecules of isodityrosine, joined by a biphenyl linkage. The possible reactions involved in the formation of this molecule in vivo are discussed, as is the possibility that it could form an interpolypeptide linkage between cell wall proteins such as extensin, and hence aid in the insolubilization of the protein in the wall.
Brady
J D
JD
Institute of Cell and Molecular Biology, University of Edinburgh, Edinburgh, Scotland, U.K.
Sadler
I H
IH
Fry
S C
SC
D000595:Amino Acid Sequence; D001713:Biphenyl Compounds / Q000032:analysis*; D002473:Cell Wall / Q000737:chemistry; D003432:Cross-Linking Reagents / Q000032:analysis*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000032:analysis*; D010944:Plants / Q000737:chemistry / Q000648:ultrastructure; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000032:analysis
D001713:Biphenyl Compounds; D003432:Cross-Linking Reagents; D010940:Plant Proteins; D014443:Tyrosine