Characterization and solubilization of testosterone 17 beta-hydroxysteroid dehydrogenase in human hyperplastic prostate.
17 beta-Hydroxysteroid dehydrogenase is a membrane-bound enzyme in human prostate. Solubilization of this enzyme can only be obtained in the presence of detergents. The optimal solubilization mixture contained 50 mM Tris-HCl buffer pH 9.0, 20% glycerol, 0.1 M KCl and 5 mg/ml of the non-ionic detergent N-octyl glucoside. In these conditions, the soluble fraction contained more than 90% of the enzymatic activity. A 2.5-fold increase of specific activity was obtained during solubilization under optimal conditions.
Carre
J L
JL
Laboratoire de Biochimie, Faculté de Médecine, Brest, France.
Quemener
E
E
Amet
Y
Y
Simon
B
B
Berthou
F
F
Mangin
P
P
Floch
H H
HH
Abalain
J H
JH
D015067:17-Hydroxysteroid Dehydrogenases / Q000737:chemistry / Q000378:metabolism*; D003902:Detergents; D006801:Humans; D008297:Male; D011467:Prostate / Q000201:enzymology*; D011470:Prostatic Hyperplasia / Q000201:enzymology*; D012995:Solubility; D013379:Substrate Specificity; D013739:Testosterone / Q000378:metabolism
D003902:Detergents; D013739:Testosterone; D015067:17-Hydroxysteroid Dehydrogenases; C044256:3 (or 17)-beta-hydroxysteroid dehydrogenase