Separation of B-3 monodesamidoinsulin from human insulin by high-performance liquid chromatography under alkaline conditions.
The separation of human insulin (HI) and related compounds such as A-21 monodesamido HI (A-21DHI) and B-3 monodesamido HI (B-3DHI) was investigated using reversed-phase HPLC and capillary zone electrophoresis (CZE). In the case of HPLC under the usual acidic conditions, whereas A-21DHI was separately eluted, B-3DHI was included in the HI peak. Then it was found that by applying a novel ODS column resistant to alkaline conditions, a good separation of B-3DHI from HI could be achieved using an alkaline eluent, and two peaks corresponding to B-3DHI were eluted in front of the HI peak.
Yomota
C
C
National Institute of Health Sciences, Osaka Branch, Japan.
Yoshii
Y
Y
Takahata
T
T
Okada
S
S
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D019075:Electrophoresis, Capillary; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007328:Insulin / Q000031:analogs & derivatives* / Q000302:isolation & purification*
D007328:Insulin; C099825:insulin, desamido (B-3)