Characterization of a rice sucrose-phosphate synthase-encoding gene.
A rice genomic clone (sps1) coding for sucrose phosphate synthase (SPS) was isolated and sequenced. Rice sps1 contains 13 exons and 12 introns, an unusually long 366-bp leader region with a highly organized primary structure and a promoter region with no obvious homology with eukaryotic promoter consensus sequences. Southern blot analysis showed that SPS is encoded by a single-copy gene in the rice genome. Comparison of the rice, maize, potato and spinach SPS deduced amino acid (aa) sequences showed that these enzymes have a well conserved region comprising their first 700 aa, and a variable C-terminal region. Analysis of rice sps1 expression showed that mRNA levels change during leaf development. SPS activity and mRNA were undetectable in roots.
Valdez-Alarcón
J J
JJ
Departamento de Ingeniería Genética de Plantas, Centro de Investigación y Estudios Avanzados del I.P.N., Unidad Irapuato, Guanajuato, Argentina.
Ferrando
M
M
Salerno
G
G
Jimenez-Moraila
B
B
Herrera-Estrella
L
L
D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D018744:DNA, Plant; D015870:Gene Expression; D018745:Genome, Plant; D005964:Glucosyltransferases / Q000235:genetics*; D008969:Molecular Sequence Data; D012275:Oryza / Q000201:enzymology* / Q000235:genetics; D018515:Plant Leaves / Q000254:growth & development / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
D018744:DNA, Plant; D005964:Glucosyltransferases; C022698:sucrose-phosphate synthase