title: Accumulation of gossypol enantiomers in ovine tissues.
abstract: Tissue residue levels of gossypol enantiomers in cottonseed-fed and lethally intoxicated lambs were determined by the high-performance liquid chromatography-ultraviolet detection method. Gossypol was derivatized with (+)-2-amino-1-propanol and separated with a reversed-phase C18 column and the elution of analytes was monitored at 254 nm. The highest residue level was found in the liver tissue (318-416 ng total gossypol/mg dry tissue), and the residue of (-)-gossypol was higher than (+)-gossypol in the heart, muscle and spleen. The detection limit was 2 ng, and the detector response of gossypol-amine adducts was linear between 2 and 100 ng enantiomers.
authors.affiliation: Department of Veterinary Physiology and Pharmacology, Texas A+M University College Station 77843, USA.
authors.forename: H L
authors.initials: HL
authors.lastname: Kim
authors.forename: M C
authors.initials: MC
authors.lastname: Calhoun
authors.forename: R D
authors.initials: RD
authors.lastname: Stipanovic
mesh_terms: D000821:Animal Feed; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003369:Cottonseed Oil; D006072:Gossypol / Q000493:pharmacokinetics* / Q000633:toxicity*; D011041:Poisoning / Q000378:metabolism / Q000662:veterinary*; D015203:Reproducibility of Results; D012756:Sheep; D012757:Sheep Diseases; D013237:Stereoisomerism; D014018:Tissue Distribution
chemical_list: D003369:Cottonseed Oil; D006072:Gossypol