A novel expression vector for transfection of bovine MHC class I genes.
A vector is described for the expression of genomic or cDNA copies of bovine major histocompatibility complex (MHC) class I genes in transfected mouse Ltk- cells. Class I gene fragments are amplified by the polymerase chain reaction, using primers in conserved parts of exon 2 and the 3'-untranslated region of the gene. Amplified class I gene fragments can then be subcloned into the expression vector, pBoLA-21, which contains the necessary 5'- and 3'-sequences for correct expression. The vector was tested by subcloning and expressing genomic and cDNA clones.
Sawhney
S M
SM
Division of Molecular Biology, Roslin Institute, Midlothian, UK.
Hopkins
J
J
Spooner
R L
RL
Russell
G C
GC
D000818:Animals; D001483:Base Sequence; D002417:Cattle / Q000235:genetics*; D003001:Cloning, Molecular; D017931:DNA Primers; D018076:DNA, Complementary / Q000096:biosynthesis; D005805:Genes, MHC Class I; D005822:Genetic Vectors / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D014162:Transfection / Q000379:methods*
D017931:DNA Primers; D018076:DNA, Complementary