Healing response to anorganic bone implantation in periodontal intrabony defects in dogs. Part I. Bone regeneration. A microradiographic study.
The purpose of the present study was to explore the regenerative potential of anorganic bone plus collagen (AB-C) in experimental intrabony defects. Eight healthy female beagle dogs, 3 to 4 years old and weighing 15 to 16 kilos, were used. After extraction of the mandibular third premolars (P3), surgical defects were created and inflammation induced by placement of cotton and steel braids. Eight weeks later, the braids were removed. The experimental lesions thus obtained were either treated by plain flap curettage (group 1: control) or were, in addition, implanted with AB-C (group 2: experimental). Blocks of AB-C alone were observed by scanning electron microscopy (SEM). The results show that the surface of the particles have the characteristics of a bone tissue. These particles are gathered together with a fibrillar network. Six, 18, and 36 weeks postoperative (PO), non-decalcified specimens from both groups were examined histologically by contact microradiography. In group 1, no significant bone regeneration was observed at 6, 18, or 36 weeks PO. In group 2, trabeculae undergoing mineralization and circumscribing dense particles above the reference notch were seen at 6 weeks PO; 18 and 36 week specimens showed significant bone regeneration with more or less dense remaining particles. The periodontal ligament space was always clear and the only signs of ankylosis noticed were deep in the notch on one 18 week group 2 specimen and on one 36 week group 1 specimen.
Clergeau
L P
LP
Department of Oral Surgery, Dental School, Nantes University, France.
Danan
M
M
Clergeau-Guérithault
S
S
Brion
M
M
D016301:Alveolar Bone Loss / Q000000981:diagnostic imaging / Q000473:pathology / Q000601:surgery*; D000818:Animals; D000844:Ankylosis / Q000473:pathology; D001641:Bicuspid; D001861:Bone Regeneration; D016025:Bone Transplantation / Q000000981:diagnostic imaging / Q000379:methods* / Q000473:pathology; D002113:Calcification, Physiologic; D003094:Collagen / Q000627:therapeutic use / Q000648:ultrastructure; D003475:Curettage; D004285:Dogs; D005260:Female; D008334:Mandible; D008852:Microradiography; D008855:Microscopy, Electron, Scanning; D010513:Periodontal Ligament / Q000473:pathology; D010518:Periodontitis / Q000601:surgery; D013499:Surface Properties; D013524:Surgical Flaps; D013997:Time Factors; D014945:Wound Healing
D003094:Collagen