Deletion mapping of gliomas suggest the presence of two small regions for candidate tumor-suppressor genes in a 17-cM interval on chromosome 10q.
The loss of genetic material on chromosome 10q is frequent in different tumors and particularly in malignant gliomas. We analyzed 90 of these tumors and found loss of heterozygosity (LOH) in >90% of the informative loci in glioblastoma multiforme (GBM). Initial studies restricted the common LOH region to 10q24-qter. Subsequently, the study of a pediatric GBM suggested D10S221 and D10S209, respectively, as centromeric and telomeric markers of a 4-cM LOH region. It is interesting to note that, in one subset of cells from this tumor, locus D10S209 seems involved in the allelic imbalance of a larger region, with D10S214 as telomeric marker. This 17-cM region contains the D10S587-D10S216 interval of common deletion recently defined on another set of gliomas.
Albarosa
R
R
Department of Biochemistry, Istituto Nazionale Neurologico C. Besta, Milan, Italy.
Colombo
B M
BM
Roz
L
L
Magnani
I
I
Pollo
B
B
Cirenei
N
N
Giani
C
C
Conti
A M
AM
DiDonato
S
S
Finocchiaro
G
G
D001932:Brain Neoplasms / Q000235:genetics* / Q000473:pathology; D002503:Centromere; D002648:Child; D002874:Chromosome Mapping; D002879:Chromosomes, Human, Pair 10; D004273:DNA, Neoplasm / Q000032:analysis; D017353:Gene Deletion; D016147:Genes, Tumor Suppressor; D005819:Genetic Markers; D015698:Genomic Library; D005910:Glioma / Q000235:genetics* / Q000473:pathology; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D008279:Magnetic Resonance Imaging; D016615:Telomere
D004273:DNA, Neoplasm; D005819:Genetic Markers