title: Disruption of CENP antigen function perturbs dynein anchoring to the mitotic kinetochore.
abstract: Injection of purified autoantibodies against human centromeric proteins into HeLa cells during interphase disrupts the organization of the kinetochore and interferes with chromosomal movements during the subsequent mitosis even though the chromosomes retain the ability to bind microtubules. We have investigated the hypothesis that this phenotype arises from effects on cytoplasmic dynein, the microtubule motor protein. In previous experiments we found that introduction of anticentromere antibodies into cell nuclei during the G1- or S-phases causes a prometaphase-like arrest, while injections during G2-phase cause a metaphase arrest. We show here that, in both cases, the level of detectable cytoplasmic dynein at kinetochores is significantly decreased. In contrast, when injected cells were permitted to enter mitosis in the absence of microtubules (conditions where trilaminar kinetochores could be detected by electron microscopy), the intensity of dynein labeling on the kinetochores was identical to that seen in uninjected control cells exposed to colcemid. Therefore, the loss of dynein label on mitotic kinetochores was correlated both with the injection of anticentromere antibodies and with the presence of intact spindle microtubules. We suggest that the injection of anticentromere antibodies somehow weakens the association of dynein with the kinetochore, so that when microtubules are present, these motor molecules are pulled away from the kinetochores as they generate force. This model offers an explanation for the failure of chromosomes of injected cells to move normally in mitosis even though they have attached microtubules.
authors.affiliation: Department of Physiology and Biophysics, SJ-40, University of Washington Medical School, Seattle, WA 98195, USA.
authors.forename: L
authors.initials: L
authors.lastname: Wordeman
authors.forename: W C
authors.initials: WC
authors.lastname: Earnshaw
authors.forename: R L
authors.initials: RL
authors.lastname: Bernat
mesh_terms: D000906:Antibodies / Q000494:pharmacology; D001324:Autoantigens / Q000235:genetics / Q000276:immunology; D002503:Centromere / Q000235:genetics / Q000276:immunology; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics / Q000276:immunology*; D003593:Cytoplasm / Q000737:chemistry; D003703:Demecolcine / Q000494:pharmacology; D004398:Dyneins / Q000737:chemistry / Q000187:drug effects / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D007074:Immunoglobulin G / Q000494:pharmacology; D018386:Kinetochores / Q000737:chemistry / Q000187:drug effects / Q000502:physiology*; D008677:Metaphase / Q000187:drug effects; D008845:Microinjections; D008870:Microtubules / Q000378:metabolism; D008938:Mitosis / Q000187:drug effects* / Q000276:immunology; D008957:Models, Genetic; D008941:Spindle Apparatus / Q000187:drug effects / Q000235:genetics; D013194:Staining and Labeling
chemical_list: D000906:Antibodies; D001324:Autoantigens; D002868:Chromosomal Proteins, Non-Histone; D007074:Immunoglobulin G; D004398:Dyneins; D003703:Demecolcine