title: A functional Spo0A is required for maximal aprE expression in Bacillus subtilis.
abstract: The initiation of sporulation in Bacillus subtilis is under control of the transcriptional factor Spo0A. Most Spo0A mutants fail to initiate the sporulation process and all the sporulation initiated processes such as the synthesis of subtilisin. However, the product of spo0A9V, one of the several spo0A mutants characterized, distinguishes itself in the fact that, while it appears to effectively repress abrB, it fails to activate the spoIIA operon. The aim of this study was to examine the effect of the spo0A9V mutation on aprE expression and we found that in different genetic backgrounds, the spo0A9V mutation has a negative effect on aprE::lacZ expression.
authors.affiliation: Molecular Microbiology Department, Instituto de Biotecnologia, Universidad Nacional Autónoma de México, Cuernavaca, Mexico.
authors.forename: J
authors.initials: J
authors.lastname: Olmos
authors.forename: V
authors.initials: V
authors.lastname: Bolaños
authors.forename: S
authors.initials: S
authors.lastname: Causey
authors.forename: E
authors.initials: E
authors.lastname: Ferrari
authors.forename: F
authors.initials: F
authors.lastname: Bollvar
authors.forename: F
authors.initials: F
authors.lastname: Valle
mesh_terms: D001412:Bacillus subtilis / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D017931:DNA Primers; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D005838:Genotype; D026901:Membrane Transport Proteins; D008969:Molecular Sequence Data; D009154:Mutation; D010957:Plasmids; D016133:Polymerase Chain Reaction; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D015183:Restriction Mapping; D013045:Species Specificity; D013171:Spores, Bacterial; D014157:Transcription Factors / Q000378:metabolism*; D001616:beta-Galactosidase / Q000096:biosynthesis
chemical_list: C088074:AprE protein, Bacteria; D001426:Bacterial Proteins; D017931:DNA Primers; D026901:Membrane Transport Proteins; D011993:Recombinant Fusion Proteins; C080950:Spo0A protein, Bacillus subtilis; D014157:Transcription Factors; D001616:beta-Galactosidase