Purification and N-terminal amino acid sequence of dextranicin 24, a bacteriocin of Leuconostoc sp.
Leuconostoc mesenteroides subsp. dextranicum strain J24 synthesized a bacteriocin named Dextranicin 24 (Dex-24), which inhibited only other Leuconostoc sp. strains. It was purified by a two-step procedure from the fraction of the bacteriocin bound to the producer cells at the end of the growth: desorption form the cells at acidic pH, followed by reserve phase HPLC. The N-terminal sequence of Dex-24 was the following: NH2(-) K G V L G W L S M A S S A L T G P Q Q . . .
Revol-Junelles
A M
AM
Laboratoire de Microbiologie Industrielle et Alimentaire, Ecole Nationale Supérieure d'Agronomie et des Industries Alimentaires, Institut National Polytechnique de Lorraine, Nancy, France.
Lefebvre
G
G
D000595:Amino Acid Sequence; D001430:Bacteriocins / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D002611:Cheese / Q000382:microbiology; D007934:Leuconostoc / Q000187:drug effects / Q000378:metabolism*; D008969:Molecular Sequence Data
D001430:Bacteriocins; C100883:dextranicin-24