title: Kaposi's sarcoma-associated herpesvirus (KSHV or HHV8) in primary effusion lymphoma: ultrastructural demonstration of herpesvirus in lymphoma cells.
abstract: Recent molecular evidence suggests an association with a new herpesvirus, Kaposi's sarcoma-associated herpesvirus (KSHV/HHV-8), and primary effusion lymphomas (PELs). PELs have a characteristic morphology, phenotype, and clinical presentation, with malignant effusions in the absence of a contiguous solid tumor mass. We have established a cell line (KS-1) from a KSHV-positive human immunodeficiency virus (HIV)-negative patient with pleural cavity-based lymphoma that was passaged into triple-immunodeficient BNX mice. In contrast to cell lines from body cavity-based lymphomas derived from HIV-positive individuals that contain both KSHV and Epstein Barr viral genome, these cells contain only KSHV, allowing for uncontaminated virologic studies. Ultrastructural examination identified malignant cells with features of late differentiating B cells (immunoblasts). Cells with viral cytopathic effect contained typical 110-nm intranuclear herpesvirus nucleocapsids and complete cytoplasmic virions, confirming the association of PEL with KSHV.
authors.affiliation: Department of Pathology, Cedars Sinai Medical Center, Los Angeles, CA 90048, USA.
authors.forename: W
authors.initials: W
authors.lastname: Said
authors.forename: K
authors.initials: K
authors.lastname: Chien
authors.forename: S
authors.initials: S
authors.lastname: Takeuchi
authors.forename: T
authors.initials: T
authors.lastname: Tasaka
authors.forename: H
authors.initials: H
authors.lastname: Asou
authors.forename: S K
authors.initials: SK
authors.lastname: Cho
authors.forename: S
authors.initials: S
authors.lastname: de Vos
authors.forename: E
authors.initials: E
authors.lastname: Cesarman
authors.forename: D M
authors.initials: DM
authors.lastname: Knowles
authors.forename: H P
authors.initials: HP
authors.lastname: Koeffler
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000648:ultrastructure / Q000821:virology; D001483:Base Sequence; D002213:Capsid / Q000648:ultrastructure; D003588:Cytopathogenic Effect, Viral; D004279:DNA, Viral / Q000032:analysis; D006566:Herpesviridae Infections / Q000821:virology*; D006801:Humans; D016393:Lymphoma, B-Cell / Q000648:ultrastructure / Q000821:virology*; D051379:Mice; D008819:Mice, Nude; D008969:Molecular Sequence Data; D014411:Neoplastic Stem Cells / Q000648:ultrastructure / Q000821:virology; D010996:Pleural Effusion / Q000821:virology*; D010997:Pleural Neoplasms / Q000648:ultrastructure / Q000821:virology*; D016133:Polymerase Chain Reaction; D018144:Rhadinovirus / Q000302:isolation & purification* / Q000648:ultrastructure; D014407:Tumor Cells, Cultured
chemical_list: D004279:DNA, Viral