Sequence and expression of a bovine herpesvirus-1 gene homologous to the glycoprotein K-encoding gene of herpes simplex virus-1.
In the bovine herpes virus-1 (BHV-1) genome, a gene equivalent to the glycoprotein k (gK)-encoding gene of other herpesviruses was identified and sequenced. The primary translation product is predicted to comprise 338 amino acids (aa) and to exhibit a molecular mass of 37.5 kDa. It possesses characteristics typical for membrane glycoproteins including a potential cleavable signal sequence, three transmembrane domains and two potential N-linked glycosylation sites. Comparison to the gK proteins of the other herpesviruses revealed aa sequence homologies of 46, 44, 53, 43, and 46% with the gK counterparts of herpes simplex viruses-1 and 2 (HSV-1 and 2), equine herpesvirus-1 (EHV-1), Marek's disease virus (MDV) and varicella zoster virus (VZV), respectively. A 30-kDa primary translation product was identified following in vitro translation of in vitro transcribed mRNA. When canine microsomal membranes were added to the translation reaction, a 38-kDa glycosylated protein was detected. Treatment with endoglycosidase F or H (endo or H) removed the glycosyl groups and reduced the apparent molecular mass of the 38-kDa glycoprotein.
Khadr
A
A
Veterinary Infectious Disease Organization, University of Saskatchewan, Saskatoon, Canada.
Tikoo
S K
SK
Babiuk
L A
LA
van Drunen Littel-van den Hurk
S
S
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D015870:Gene Expression; D005814:Genes, Viral; D016679:Genome, Viral; D006031:Glycosylation; D006564:Herpesviridae / Q000235:genetics; D007242:Herpesvirus 1, Bovine / Q000235:genetics* / Q000378:metabolism; D004861:Herpesvirus 1, Equid / Q000235:genetics; D018259:Herpesvirus 1, Human / Q000235:genetics* / Q000378:metabolism; D006736:Horses; D006801:Humans; D008861:Microsomes / Q000378:metabolism; D008969:Molecular Sequence Data; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D017386:Sequence Homology, Amino Acid; D014764:Viral Proteins / Q000096:biosynthesis / Q000235:genetics*
D012333:RNA, Messenger; C072448:UL53 protein, Human herpesvirus 1; D014764:Viral Proteins