[Urate production in a porcine model of myocardial ischemia-reperfusion].
OBJECTIVE
This study was designed to investigate urate production by swine hearts using an in vivo regionally ischemic-reperfused model.


METHODS
Ten female pigs underwent 60 minutes of myocardial ischemia by clamping of the left anterior descending artery and afterwards 120 minutes of reperfusion. Epicardial biopsies and blood samples from coronary sinus were taken before ligation, at the end of ischemic period and 5, 30, 60 and 120 minutes upon reperfusion.


RESULTS
During ischemia, tissue levels of ATP and ADP greatly declined with a subsequent increase in the concentration of AMP, inosine and hypoxanthine (33 +/- 12 vs 93 +/- 17, 26 +/- 8 vs 768 +/- 86 and 32 +/- 10 vs 219 +/- 26 nmol/g dry weight, p < 0.01 for each). Despite the great increase in the hypoxanthine levels, uric acid concentration remained constant (69 +/- 9 vs 32 +/- 12 nmol/g dry weight, NS). Hypoxanthine, xanthine and uric acid concentrations increased in blood samples obtained from the coronary sinus at the end of ischemic period (17.99 vs 31.03 nmol/ml, p < 0.01, 0.29 vs 1.45 nmol/ml, p < 0.05 and 1.20 vs 2.31 nmol/ml, p < 0.01 respectively) and were enhanced upon reperfusion (35.8 and 3.89 nmol/ml for hypoxanthine and uric acid respectively, p < 0.05) without any significant modifications in their concentrations at the arterial level.


CONCLUSIONS
These results demonstrate that the ischemic-reperfused swine heart produces urate probably outside the myocardium.
Martín-Jadraque
R
R
Instituto de Investigaciones Biomédicas del CSIC, Universidad Autónoma de Madrid.
Montero
C
C
Mostaza
J M
JM
López-Sendón
J L
JL
Argomaniz
L
L
Llorente
P
P
Martín-Jadraque
L
L
D000818:Animals; D005260:Female; D005609:Free Radicals; D019271:Hypoxanthine; D007042:Hypoxanthines / Q000097:blood; D015428:Myocardial Reperfusion Injury / Q000097:blood / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism; D013552:Swine; D014527:Uric Acid / Q000097:blood / Q000378:metabolism*; D019820:Xanthine; D014970:Xanthines / Q000097:blood
D005609:Free Radicals; D007042:Hypoxanthines; D014970:Xanthines; D019820:Xanthine; D014527:Uric Acid; D019271:Hypoxanthine
Producción de urato en un modelo porcino de isquemia-reperfusion miocárdica.