title: The pel1 mutant of Saccharomyces cerevisiae is deficient in cardiolipin and does not survive the disruption of the CHO1 gene encoding phosphatidylserine synthase.
abstract: Cells of the pel1 mutant of Saccharomyces cerevisiae were found to contain an extremely low content of cardiolipin, a decreased level of phosphatidylcholine and an increased level of phosphatidylinositol. Disruption of the PEL1 gene in cells containing a null mutation in the CHO1 gene was lethal. Despite its putative functional homology with CHO1, the overexpression of the PEL1 gene in the cho1 null mutant did not restore the wild-type properties of the transformed cells and failed to stimulate the incorporation of L-[3-3H]serine into total lipids of the intact yeast cells.
authors.affiliation: Comenius University, Department of Microbiology and Virology, Bratislava, Slovak Republic.
authors.forename: M
authors.initials: M
authors.lastname: Janitor
authors.forename: M
authors.initials: M
authors.lastname: Obernauerová
authors.forename: S D
authors.initials: SD
authors.lastname: Kohlwein
authors.forename: J
authors.initials: J
authors.lastname: Subík
mesh_terms: D000941:Antigens / Q000235:genetics; D010717:CDPdiacylglycerol-Serine O-Phosphatidyltransferase / Q000235:genetics*; D002308:Cardiolipins / Q000235:genetics / Q000378:metabolism*; D005656:Fungal Proteins / Q000235:genetics*; D005800:Genes, Fungal / Q000502:physiology; D050356:Lipid Metabolism; D008869:Microtubule-Associated Proteins / Q000235:genetics*; D009154:Mutation / Q000502:physiology; D010641:Phenotype; D010718:Phosphatidylserines / Q000502:physiology; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins
chemical_list: D000941:Antigens; C490519:CHO1 protein, S cerevisiae; D002308:Cardiolipins; D005656:Fungal Proteins; D008869:Microtubule-Associated Proteins; D010718:Phosphatidylserines; D029701:Saccharomyces cerevisiae Proteins; D010717:CDPdiacylglycerol-Serine O-Phosphatidyltransferase; C084652:PGS1 protein, S cerevisiae