Rotational motion of Ca-ATPase monitored by electron spin echoes.
Electron spin echoes are used to study the dynamics of different aggregational forms of spin-labeled Ca-ATPase in the sarcoplasmic reticulum membrane. The 2D-ESE measurements are sensitive to motions on the microsecond time scale. The motional information is extracted from the variation of the echo decays across the CW-ESR absorption spectrum. The motional contribution to the decays is described by assuming that the Ca-ATPase molecule is perfectly oriented along the normal to the membrane surface and only undergoes rotational motion about its long axis. The echo-amplitude decays have been evaluated in the time domain by solving the Bloch equations for the stochastic spin Hamiltonian on making use of stochastic trajectories for the orientational behavior of the spin-labeled protein. This approach provides a useful insight into the information provided by the 2D-ESE measurements and affords a direct comparison of the results obtained with different experimental techniques. It is shown that the 2D-ESE technique monitors the orientational motions of dimers or larger aggregates of Ca-ATPase molecules whose rotational correlation times vary between 200 microseconds and 1 ms for the temperature range between 37 and 4 degrees C.
van der Struijf
C
C
Department of Molecular Biophysics, Debye Research Center, Utrecht, The Netherlands.
Pelupessy
T P
TP
van Faassen
E E
EE
Levine
Y K
YK
D000818:Animals; D000252:Calcium-Transporting ATPases / Q000032:analysis*; D004578:Electron Spin Resonance Spectroscopy; D008968:Molecular Conformation; D011817:Rabbits; D012519:Sarcoplasmic Reticulum / Q000201:enzymology*; D013113:Spin Labels
D013113:Spin Labels; D000252:Calcium-Transporting ATPases