title: Cross-reactivity between the major allergen from olive pollen and unrelated glycoproteins: evidence of an epitope in the glycan moiety of the allergen.
abstract: Ole e 1, the major allergen from olive pollen, is a glycoprotein containing a single Asn-linked glycan moiety. Rabbit antiserum against this protein has been obtained; and its immunologic cross-reactivities in Western blotting with ascorbate oxidase, horseradish peroxidase, bromelain, ovalbumin, and honeybee venom phospholipase A2 have been studied. Ascorbate oxidase, peroxidase, and bromelain are recognized by the Ole e 1 antiserum. When these three proteins are deglycosylated by periodate treatment, such an immunologic reaction does not occur. The relative affinities of these proteins have been analyzed by direct and inhibition ELISA experiments. A commercially available antibody against horseradish peroxidase has also been considered in these studies. This antibody reacts with Ole e 1 but not with the periodate-deglycosylated allergen. Horseradish peroxidase, bromelain, and ascorbate oxidase are recognized by the IgE of sera from patients who are hypersensitive to olive tree pollen. This binding is also abolished by periodate treatment. The results are interpreted in terms of the presence of an epitope in the carbohydrate moiety of Ole e 1, which would contain a xylose involved in recognition by both IgE and IgG antibodies.
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Facultad de Química, Universidad Complutense, Madrid, Spain.
authors.forename: E
authors.initials: E
authors.lastname: Batanero
authors.forename: M
authors.initials: M
authors.lastname: Villalba
authors.forename: R I
authors.initials: RI
authors.lastname: Monsalve
authors.forename: R
authors.initials: R
authors.lastname: Rodríguez
mesh_terms: D000485:Allergens / Q000737:chemistry / Q000276:immunology*; D000818:Animals; D000915:Antibody Affinity; D002240:Carbohydrate Sequence; D003429:Cross Reactions; D000939:Epitopes; D006023:Glycoproteins / Q000276:immunology*; D006801:Humans; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010940:Plant Proteins / Q000276:immunology; D011058:Pollen / Q000737:chemistry / Q000276:immunology*; D011817:Rabbits; D013329:Structure-Activity Relationship; D014197:Trees / Q000276:immunology*
chemical_list: D000485:Allergens; D000939:Epitopes; D006023:Glycoproteins; D010940:Plant Proteins