title: Expression of a novel member of estrogen response element-binding nuclear receptors is restricted to the early stages of chorion formation during mouse embryogenesis.
abstract: Members of the nuclear hormone receptor gene family of transcription factors have been shown to be expressed in characteristic patterns during mouse organogenesis and postnatal development. Using an RT-PCR based screening assay, we have identified nuclear receptors expressed in embryonal carcinoma stem cells. One of the cDNAs characterized, mERR-2, was found to be expressed exclusively during a narrow developmental window in trophoblast progenitor cells between days 6.5 and 7.5 post coitum (p.c.). From 8.5 days p.c. and onwards, the mERR-2 gene activity evaded detection as analysed by in situ hybridization. We also show that the mERR-2 gene product and the estrogen receptor share a common target DNA-sequence recognition specificity unique among members of the gene family. Furthermore, efficient homodimerization and DNA-binding of the orphan receptor mERR-2 was found to be dependent on interaction with the heat shock protein 90, a molecular chaperone hitherto recognized to interact only with the steroid hormone receptor subgroup of nuclear receptors. Based on our results we suggest that the mouse orphan receptor mERR-2 has the potential to regulate overlapping gene networks with the estrogen receptor and may participate in signal transduction pathways during a short developmental period coinciding with the formation of the chorion.
authors.affiliation: Karolinska Institute, Department of Medical Nutrition, F60 NOVUM, Huddinge, Sweden.
authors.forename: K
authors.initials: K
authors.lastname: Pettersson
authors.forename: K
authors.initials: K
authors.lastname: Svensson
authors.forename: R
authors.initials: R
authors.lastname: Mattsson
authors.forename: B
authors.initials: B
authors.lastname: Carlsson
authors.forename: R
authors.initials: R
authors.lastname: Ohlsson
authors.forename: A
authors.initials: A
authors.lastname: Berkenstam
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002454:Cell Differentiation; D002460:Cell Line; D002823:Chorion / Q000196:embryology*; D004247:DNA / Q000378:metabolism; D018076:DNA, Complementary; D005314:Embryonic and Fetal Development / Q000235:genetics*; D005260:Female; D006360:Heat-Shock Proteins / Q000378:metabolism; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D011485:Protein Binding; D012333:RNA, Messenger / Q000235:genetics; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics* / Q000378:metabolism; D011960:Receptors, Estrogen / Q000235:genetics* / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; C098925:Esrrb protein, mouse; D006360:Heat-Shock Proteins; D012333:RNA, Messenger; D018160:Receptors, Cytoplasmic and Nuclear; D011960:Receptors, Estrogen; D004247:DNA