The structure and expression of an hsc70 gene from Lycopersicon esculentum.
A tomato hsc70 genomic clone (Lehsc70-3; Lycopersicon esculentum heat-shock cognate 70-3) was obtained by screening a genomic library with the tomato Lehsc70-2 cDNA. Two restriction fragments of 2.6 and 5 kb, which compose the Lehsc70-3 gene, were subcloned into pBluescriptIIKS+ and analyzed. Transcript mapping reveals that the mature Lehsc70-3 mRNA contains a 122-nt 5' untranslated region (UTR), a coding region of 1956 nt corresponding to a polypeptide of 651 amino acids, an intron of 717 nt and a 3' UTR. Analysis of genomic DNA indicates that Lehsc70-3 is present as a low-copy-number gene. We note that the sequence upstream from the coding region of Lehsc70-3 shares common features with a number of hsc/hsp genes. High-temperature treatment (37 degrees C) caused a twofold increase in the level of the Lehsc70-3 mRNA. However, Lehsc70-3 has also been expressed at substantial levels in tomato vegetative tissues, suggesting a general function of this hsc70 gene in L. esculentum.
Sun
S W
SW
Department of Life Science, National Tsing Hua University, Hsinchu, Taiwan, R.O.C.
Chung
M C
MC
Lin
T Y
TY
D000595:Amino Acid Sequence; D001483:Base Sequence; D018744:DNA, Plant; D018628:Gene Dosage; D015870:Gene Expression; D017343:Genes, Plant; D050883:HSC70 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics*; D007438:Introns; D018551:Solanum lycopersicum / Q000235:genetics*; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics*; D014158:Transcription, Genetic
D018744:DNA, Plant; D050883:HSC70 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; D010940:Plant Proteins; C099901:hsc70-3 protein, Lycopersicon esculentum