title: Expression of human immunodeficiency virus type 1 reverse transcriptase in trans during virion release and after infection.
abstract: The normal reverse transcription of retroviral RNA is a complex process which depends on the orchestration of several steps throughout the virus life cycle. During the assembly of retroviruses, reverse transcriptase (RT) is directed into the virion as a component of the Gag-Pol polyprotein. In the maturation of the Gag-Pol polyprotein of human immunodeficiency virus type 1 (HIV-1), cleavage by the viral protease occurs during viral budding. After infection, reverse transcription of viral RNA into double-stranded DNA is completed in the cytoplasm of the infected cell. In this study, the processing and reverse transcription of HIV-1 have been examined by separate expression of mature HIV-1 RT and proviral molecules bearing RT mutations. The effects of RT expression in trans during virion release and after viral entry were investigated. Constitutive expression of HIV-1 RT was established in CD4- and non-CD4-expressing cells via the coexpression of its individual subunits, and three HIV-1 RT mutant constructs were generated. The results indicate that a bona fide RT trans complementation does not occur during virion release or after infection. However, after infection of an RT-expressing cell with a high titer RT-defective virus, intracellular reverse transcription can be detected.
authors.affiliation: Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA.
authors.forename: M A
authors.initials: MA
authors.lastname: Ansari-Lari
authors.forename: R A
authors.initials: RA
authors.lastname: Gibbs
mesh_terms: D001483:Base Sequence; D002460:Cell Line; D015685:Fusion Proteins, gag-pol / Q000502:physiology; D016325:HIV Long Terminal Repeat; D054303:HIV Reverse Transcriptase; D015497:HIV-1 / Q000502:physiology*; D008969:Molecular Sequence Data; D009154:Mutation; D012194:RNA-Directed DNA Polymerase / Q000096:biosynthesis*; D018626:Retroelements; D014771:Virion / Q000502:physiology*; D019065:Virus Assembly
chemical_list: D015685:Fusion Proteins, gag-pol; D018626:Retroelements; D054303:HIV Reverse Transcriptase; D012194:RNA-Directed DNA Polymerase