Microdetermination of proteins by resonance light scattering spectroscopy with bromophenol blue.
Reaction of bromophenol blue with proteins results in an enhanced resonance light scattering at 334 nm. Based on this, a new quantitative determination method for proteins in the aqueous solution is established. This assay is characterized by high sensitivity (0.34-18.7 microg/ml), short reaction time (>2 min), and simplicity (a one-step assay). Due to protein-to-protein variability, this method gave results higher than that of the bromocresol green assay in detection of human serum albumin.
Ma
C Q
CQ
Department of Chemistry, Peking University, Beijing, 100871, China.
Li
K A
KA
Tong
S Y
SY
D000596:Amino Acids / Q000097:blood; D001774:Blood Chemical Analysis / Q000379:methods*; D001798:Blood Proteins / Q000032:analysis*; D001978:Bromphenol Blue; D004396:Coloring Agents; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008027:Light; D012542:Scattering, Radiation; D012709:Serum Albumin / Q000032:analysis
D000596:Amino Acids; D001798:Blood Proteins; D004396:Coloring Agents; D012709:Serum Albumin; D001978:Bromphenol Blue