Dibutyl phthalate purged autologous bone marrow transplant in the treatment of leukemia.
It has been proved that di-N-butyl phthalate (DBP) is singular in killing leukemic cells selectively or accelerating the deterioration of residual leukemic cells in long-term marrow culture in vitro. Based on this principle, the DBP-purged autologous bone marrow transplant has been applied to the treatment of a group of 14 patients suffering from acute nonlymphocytic leukemia. After 5-10 days of in vitro co-culture of marrow cells with DBP at a concentration of 50 micrograms/ml, the recovery of total nucleated cells and the amount of CFU-GM were 67.5% and 68.1%, respectively. In all patients, the reconstitution of hematopoiesis was observed after pre-conditioning and transfusion of purged marrow cells. Among these, two patients had a relapse, two patients died from complications of transplant, one patient died from non-leukemic disease, and the others are all alive and free of disease; the mean survival time as calculated recently was 15 months. These preliminary clinical data support that marrow culture in the presence of DBP is a safe and effective measure for treating leukemia in purged autologous bone marrow transplant.
Wang
L
L
Department of Experimental Hematology, Beijing Institute of Radiation Medicine, People's Republic of China.
Wu
C
C
Pei
X
X
Xu
L
L
Cao
J
J
Xue
H
H
Yang
K
K
Teng
A
A
Ji
S
S
D000293:Adolescent; D000328:Adult; D016465:Bone Marrow Purging; D016026:Bone Marrow Transplantation; D002648:Child; D003993:Dibutyl Phthalate / Q000494:pharmacology*; D018572:Disease-Free Survival; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D015470:Leukemia, Myeloid, Acute / Q000628:therapy*; D008297:Male; D014182:Transplantation, Autologous
D003993:Dibutyl Phthalate