title: Rapid and simple method for determination of cholesterol in processed food.
abstract: An improved method for determination of cholesterol in processed food with only one extraction and without solvent removal was developed. Total time to analyze a sample including gas chromatographic (GC) analysis is 45 min. Food samples spiked with internal standard are hydrolyzed in a screw-capped vial with saturated methanolic KOH. Cyclohexane is added to the mixture, and the upper layer is analyzed by GC on a capillary column. Average recoveries of spiked white eggs are 99 +/- 0.5%. Fifteen types of processed food containing shrimp, fish, meat, cheese, eggs, and vegetables were analyzed with this method and with the AOAC method.
authors.affiliation: Kuwait Institute for Scientific Research, Central Analytical Laboratory, Safat, Kuwait.
authors.forename: E D
authors.initials: ED
authors.lastname: Naeemi
authors.forename: N
authors.initials: N
authors.lastname: Ahmad
authors.forename: T K
authors.initials: TK
authors.lastname: al-Sharrah
authors.forename: M
authors.initials: M
authors.lastname: Behbahani
mesh_terms: D002776:Cholestanes / Q000032:analysis; D002791:Cholesterol, Dietary / Q000032:analysis*; D002849:Chromatography, Gas / Q000379:methods*; D005504:Food Analysis; D005511:Food Handling; D012015:Reference Standards; D015203:Reproducibility of Results; D013997:Time Factors
chemical_list: D002776:Cholestanes; D002791:Cholesterol, Dietary