B-type natriuretic peptide isolated from frog cardiac ventricles.
A new natriuretic peptide with 27 amino acid residues has been isolated from cardiac ventricles of the bullfrog, Rana catesbeiana. Since this ventricular peptide had high sequence identity to B-type (brain) natriuretic peptide (BNP), especially to chicken BNP (74%), we named it bullfrog BNP. Thus, semi-aquatic amphibians have tetrapod-type BNP, but do not seem to have fish-type ventricular natriuretic peptide (VNP) in their ventricles. Compared with other known BNPs, the C-terminus of bullfrog BNP was elongated by two amino acid residues and was not amidated. Bullfrog BNP dose-dependently decreased arterial blood pressure in the bullfrog with a potency twofold greater than that of human ANP. Bullfrog BNP also exhibited vasodepressor, natriuretic and diuretic activities in the rat, but it was 1/3, 1/7, and 1/17 as potent as human ANP in this mammalian species.
Fukuzawa
A
A
Ocean Research Institute, University of Tokyo, Japan.
Watanabe
T X
TX
Itahara
Y
Y
Nakajima
K
K
Yoshizawa-Kumagaye
K
K
Takei
Y
Y
D000595:Amino Acid Sequence; D000818:Animals; D009320:Atrial Natriuretic Factor / Q000494:pharmacology; D002417:Cattle; D002645:Chickens; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D004231:Diuresis / Q000187:drug effects; D004285:Dogs; D005260:Female; D005399:Fishes; D006352:Heart Ventricles; D006801:Humans; D008297:Male; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009206:Myocardium / Q000737:chemistry*; D020097:Natriuretic Peptide, Brain; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D011892:Rana catesbeiana; D051381:Rats; D017207:Rats, Sprague-Dawley; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D013552:Swine
D009419:Nerve Tissue Proteins; D020097:Natriuretic Peptide, Brain; D009320:Atrial Natriuretic Factor