PrP27-30 is a normal soluble prion protein fragment released by human platelets.
Prion diseases are neurodegenerative disorders characterized by the accumulation of abnormal isoforms of prion protein (PrPSc) in the central nervous system. PrPSc isoforms differ from their normal homologue (PrPC), in that they possess increased beta-sheet conformation, are partially protease resistant and may be associated with amyloid deposition. Amyloid proteins are thought to derive from soluble precursors or fragments thereof, present in biological fluids, which in the disease state undergo conformational change leading to aggregation and deposition in target tissues. We report here that platelets carry PrP mRNA and release PrPC, a sialoglycoprotein bound to the cell surface by a glycosylphosphatidylinositol (GPI) anchor. Soluble PrPC, and a N-terminal truncated PrPC isoform starting at position 90 are secreted by resting and agonist-stimulated platelets and are detectable after partial deglycosylation of releasates. N-terminal sequence analysis of the soluble 27-30 kDa isoform, GQGGGTHSQ(W)NKP, revealed homology to scrapie PrP27-30, the protease resistant core derived from PrPSc. These findings indicate that in addition to PrPC, platelets process a soluble PrP27-30 isoform. Whether this isoform can be converted in scrapie PrP27-30 remains to be determined.
Perini
F
F
Department of Pathology, New York University Medical Center, New York 10016, USA.
Vidal
R
R
Ghetti
B
B
Tagliavini
F
F
Frangione
B
B
Prelli
F
F
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001792:Blood Platelets / Q000502:physiology*; D017931:DNA Primers; D006801:Humans; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000097:blood / Q000737:chemistry; D016133:Polymerase Chain Reaction; D018622:PrP 27-30 Protein / Q000097:blood* / Q000737:chemistry; D011328:Prions / Q000097:blood* / Q000737:chemistry; D012313:RNA / Q000097:blood; D012608:Scrapie; D017386:Sequence Homology, Amino Acid
D017931:DNA Primers; D010446:Peptide Fragments; D011328:Prions; D018622:PrP 27-30 Protein; D012313:RNA