Plasma serotonin and platelet aggregation during ischemia-reperfusion in dogs: effect of dipyridamole and coenzyme Q10.
Platelet aggregation and plasma serotonin were studied during ischemia-reperfusion of the small intestine in dogs. Blood was withdrawn from the superior mesenteric vein before and 1 h after ischemia, then 5, 30 and 60 min after reperfusion. Dipyridamole (5 mg/kg body weight) and coenzyme Q10 (CoQ10; 10 mg/kg body weight) were administered intravenously 5 min before reperfusion, following 1 h ischemia, in order to investigate their effects on platelet function and free serotonin. Ischemia-reperfusion resulted in an increased local free serotonin concentration together with an enhanced platelet response to ADP, collagen and arachidonic acid. Administration of dipyridamole and CoQ10 prior to reperfusion prevented, at least in part, augmented platelet activation and serotonin release. It appeared that dipyridamole was more potent than CoQ10. Our results may indicate a possible protective effect of dipyridamole on enhanced platelet activation during ischemia-reperfusion in dogs.
Choudhury
N A
NA
Second Department of Surgery, Hamamatsu University School of Medicine, Japan.
Malyszko
J
J
Ahmed
M H
MH
Pietraszek
M H
MH
Nakamura
S
S
Nakai
K
K
Rashid
M A
MA
Takada
A
A
Baba
S
S
D000818:Animals; D003067:Coenzymes; D004176:Dipyridamole / Q000494:pharmacology*; D004285:Dogs; D004353:Drug Evaluation, Preclinical; D007422:Intestines / Q000098:blood supply*; D008297:Male; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*; D015427:Reperfusion Injury / Q000097:blood*; D012701:Serotonin / Q000097:blood / Q000502:physiology*; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000494:pharmacology
D003067:Coenzymes; D010975:Platelet Aggregation Inhibitors; D014451:Ubiquinone; D012701:Serotonin; D004176:Dipyridamole; C024989:coenzyme Q10