Significant induction of a 54-kDa protein in rat liver with homologous alignment to mouse selenium binding protein by a coplanar polychlorinated biphenyl, 3,4,5,3',4'-pentachlorobiphenyl and 3-methylcholanthrene.
A 54-kDa protein in rat liver cytosol was significantly induced by treatment with 3,4,5,3',4'-pentachlorobiphenyl (25 mg/kg, single i.p.) and 3-methylcholanthrene (20 mg/kg, once a day for 3 days, i.p.). The protein exhibited pI of 6.8 on two-dimensional gel electrophoresis. The amino acid sequences of peptide fragments from the protein digested in situ were highly similar to a selenium binding protein in mice and to the isoform acetaminophen binding protein in mice. The present result clearly demonstrates that a coplanar polychlorinated biphenyl and 3-methylcholanthrene are responsible for induction of selenium binding protein homologues. The physiological role of the mouse proteins, however, is not yet elucidated.
Ishii
Y
Y
Faculty of Pharmaceutical Sciences, Kyushu University-62, Fukuoka, Japan.
Hatsumura
M
M
Ishida
T
T
Ariyoshi
N
N
Oguri
K
K
D000595:Amino Acid Sequence; D000818:Animals; D002273:Carcinogens / Q000378:metabolism / Q000633:toxicity*; D002352:Carrier Proteins / Q000032:analysis / Q000187:drug effects* / Q000235:genetics; D003600:Cytosol / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D008099:Liver / Q000737:chemistry / Q000187:drug effects*; D008297:Male; D008748:Methylcholanthrene / Q000633:toxicity*; D008969:Molecular Sequence Data; D011078:Polychlorinated Biphenyls / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar; D012643:Selenium / Q000032:analysis*; D051137:Selenium-Binding Proteins
D002273:Carcinogens; D002352:Carrier Proteins; C000622413:Selenbp1 protein, rat; D051137:Selenium-Binding Proteins; D008748:Methylcholanthrene; D011078:Polychlorinated Biphenyls; D012643:Selenium; C023035:3,4,5,3',4'-pentachlorobiphenyl