title: Production of thermostable direct hemolysin by Vibrio parahaemolyticus enhanced by conjugated bile acids.
abstract: The effects of conjugated bile acids, glycocholic acid, and taurocholic acid (TC) on production of thermostable direct hemolysin (TDH) by Vibrio parahaemolyticus were determined by a reversed passive latex agglutination assay against TDH. The amount of TDH excreted in growth medium containing either glycocholic acid or taurocholic acid (5 mM/liter) was, on a per-cell basis, 4- to 16-fold greater than that excreted in medium without the bile acids. The amounts of TDH released from lysed cells grown with the bile acids (5 mM/liter) were 4- to 32-fold greater than those from lysed cells grown without, suggesting that the bile acids enhanced synthesis of TDH within bacterial cells. These data imply that the conjugated bile acids play a key role in the pathogenicity of V. parahaemolyticus.
authors.affiliation: Department of Bacteriology and Pathology, Kanagawa Prefectural Public Health Laboratory, Yokohama, Japan.
authors.forename: R
authors.initials: R
authors.lastname: Osawa
authors.forename: S
authors.initials: S
authors.lastname: Yamai
mesh_terms: D006000:Glycocholic Acid / Q000494:pharmacology*; D006460:Hemolysin Proteins / Q000096:biosynthesis*; D006358:Hot Temperature; D013656:Taurocholic Acid / Q000494:pharmacology*; D014736:Vibrio parahaemolyticus / Q000378:metabolism* / Q000472:pathogenicity
chemical_list: D006460:Hemolysin Proteins; D013656:Taurocholic Acid; D006000:Glycocholic Acid