Detection of t(2;5)(p23;q35) translocation by reverse transcriptase polymerase chain reaction and in situ hybridization in CD30-positive primary cutaneous lymphoma and lymphomatoid papulosis.
The t(2;5) generates a chimeric NPM-ALK transcript encoded by the nucleophosmin NPM gene fused to the anaplastic lymphoma kinase gene ALK. Using a reverse transcriptase nested polymerase chain reaction assay we have detected NPM-ALK transcripts within CD30+ primary cutaneous lymphoma and lymphomatoid papulosis (LP). The t(2;5) was identified in 4 out of 9 CD30+ anaplastic lymphomas and in 1 out of 4 CD30+ pleomorphic lymphomas. Moreover, the t(2;5) was detected in 3 out of 10 LPs. All NPM-ALK-positive lymphomas and 1 NPM-ALK-positive LP exhibited a clonal rearrangement of the T cell receptor gamma-chain gene. The t(2;5) was detected in 2 cases of LP without other evidence for a clonal lymphoid population. To identify cells carrying the t(2;5) translocation, we used immunohistochemistry to detect the ALK-encoded p80 protein and in situ hybridization for the specific detection of NPM-ALK transcripts. Both p80 protein and NPM-ALK transcripts were expressed by anaplastic or large CD30+ lymphoma cells with positive NPM-ALK amplification. The presence of t(2;5) in a subset of CD30+ cutaneous lymphoma and LP may indicate a common pathogenesis with a subset of anaplastic nodal lymphoma.
Beylot-Barry
M
M
Department of Pathology, CHU de Bordeaux et Université de Bordeaux II, France.
Lamant
L
L
Vergier
B
B
de Muret
A
A
Fraitag
S
S
Delord
B
B
Dubus
P
P
Vaillant
L
L
Delaunay
M
M
MacGrogan
G
G
Beylot
C
C
de Mascarel
A
A
Delsol
G
G
Merlio
J P
JP
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000951:Antigens, Neoplasm / Q000032:analysis; D001483:Base Sequence; D002889:Chromosomes, Human, Pair 2 / Q000235:genetics*; D002895:Chromosomes, Human, Pair 5 / Q000235:genetics*; D004273:DNA, Neoplasm / Q000235:genetics; D015321:Gene Rearrangement; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization / Q000379:methods; D015603:Keratinocytes / Q000737:chemistry; D017730:Ki-1 Antigen / Q000032:analysis; D017728:Lymphoma, Large-Cell, Anaplastic / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D017731:Lymphomatoid Papulosis / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D008875:Middle Aged; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000032:analysis; D010641:Phenotype; D016133:Polymerase Chain Reaction / Q000379:methods; D012194:RNA-Directed DNA Polymerase; D011993:Recombinant Fusion Proteins / Q000032:analysis / Q000235:genetics; D012189:Retrospective Studies; D014178:Translocation, Genetic / Q000235:genetics*
D000951:Antigens, Neoplasm; D004273:DNA, Neoplasm; D017730:Ki-1 Antigen; D009363:Neoplasm Proteins; D011993:Recombinant Fusion Proteins; C036987:protein p80; D012194:RNA-Directed DNA Polymerase