The effect of roxithromycin on the generation of reactive oxygen species in vitro.
The effect of roxithromycin (ROM), a new oral semi-synthetic macrolide, on the generation of reactive oxygen species (ROS), using human neutrophils and a cell-free, xanthine-xanthine oxidase system was examined. The species investigated were the superoxide radical anion (O2-), hydrogen peroxide (H2O2), and the hydroxyl radical (OH.). ROM effectively inhibited the generation of O2-, H2O2 and OH. by human neutrophils. On the other hand, the drug did not markedly affect the ROS levels generated in the xanthine-xanthine oxidase system. The present study indicates that ROM may exert an anti-inflammatory action by inhibiting neutrophil oxygen radical generation at the sites of inflammation.
Akamatsu
H
H
Department of Dormatology, Kansai Medical University, Osaka, Japan.
Nishijima
S
S
Akamatsu
M
M
Kurokawa
I
I
Sasaki
H
H
Asada
Y
Y
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002474:Cell-Free System; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D017665:Hydroxyl Radical / Q000378:metabolism; D066298:In Vitro Techniques; D018836:Inflammation Mediators / Q000378:metabolism; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism*; D015575:Roxithromycin / Q000494:pharmacology*; D013481:Superoxides / Q000378:metabolism; D019820:Xanthine; D014969:Xanthine Oxidase / Q000378:metabolism; D014970:Xanthines / Q000378:metabolism
D000900:Anti-Bacterial Agents; D018836:Inflammation Mediators; D017382:Reactive Oxygen Species; D014970:Xanthines; D013481:Superoxides; D019820:Xanthine; D015575:Roxithromycin; D017665:Hydroxyl Radical; D006861:Hydrogen Peroxide; D014969:Xanthine Oxidase