Analysis of neutral glycosphingolipids of Theileria sergenti piroplasms.
Neutral glycosphingolipids (GSLs) were isolated from a lipid extract of Theileria sergenti piroplasms and analyzed by thin-layer chromatography (TLC) and liposome immune lysis assay (LILA); two predominant GSLs, designated as N-1 and N-2 were separated on TLC. N-1 GSL showed the same mobility as lactosylceramide (LacCer) on the TLC plate. On the other hand, the mobility of N-2 GSL on the TLC plate was identical to that of galactosylparagloboside. In order to characterize the molecular species of neutral GSLs from T. sergenti, N-1 and N-2 GSLs were tested by LILA with antibodies against LacCer and galactosylparagloboside, respectively, N-1 GSL had reactivity to anti-LacCer antibody and N-2 reacted with the antibody to galactosylparagloboside. These results suggest that N-1 and N-2 GSLs are LacCer (Gal beta 1-4Glc beta 1-1Cer) and galactosylparagloboside (Gal alpha 1-3Gal beta 1-4GlcNAc beta 1-3Gal beta 1-4Glc beta 1-1Cer), respectively.
Watarai
S
S
Department of Cell Chemistry, Okayama University Medical School, Japan.
Sugimoto
C
C
Onuma
M
M
Kobayashi
K
K
Yasuda
T
T
D000818:Animals; D000913:Antibodies, Protozoan / Q000276:immunology; D002240:Carbohydrate Sequence; D002417:Cattle; D002855:Chromatography, Thin Layer / Q000379:methods / Q000662:veterinary; D006028:Glycosphingolipids / Q000032:analysis* / Q000737:chemistry / Q000276:immunology; D007106:Immune Sera / Q000276:immunology; D008969:Molecular Sequence Data; D016794:Theileria / Q000737:chemistry* / Q000276:immunology; D013801:Theileriasis / Q000276:immunology
D000913:Antibodies, Protozoan; D006028:Glycosphingolipids; D007106:Immune Sera