[Amplification of oncogene c-erb-B1 and cellular DNA content in squamous cell carcinoma of the head and neck].
Tumoral DNA content was studied by flow cytometry and PCR amplification of c-erbB1 in tissue samples from 31 patients with squamous cell carcinoma of the head and neck. Eighteen cases (58%) were aneuploid and 13 (42%) were diploid. Aneuploidy correlated with pharyngeal site and poorly differentiated tumors, but not with clinical stage or metastases. Six (19.3%) cases had c-erbB1 amplification, which correlated with tumor size, nodal metastasis, poor differentiation, and hypopharyngeal site. Only 20% of patients with amplification survived 30 months, compared with 64% of patients without amplification. None of the patients with aneuploidy and c-erbB1 amplification survived more than 15 months. To conclude, the measurement of cellular DNA content and c-erbB1 amplification seem to have prognostic value in squamous cell carcinoma of the head and neck.
Rodrigo Tapia
J P
JP
Servicio de ORL, Hospital Central de Asturias.
Martínez Sánchez
J A
JA
Sánchez Lazo
P
P
Ramos
S
S
Sampedro Nuño
A
A
Suárez Nieto
C
C
D000328:Adult; D000368:Aged; D001483:Base Sequence; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology*; D066246:ErbB Receptors / Q000235:genetics*; D005260:Female; D005434:Flow Cytometry; D005784:Gene Amplification; D006801:Humans; D007012:Hypopharyngeal Neoplasms / Q000235:genetics* / Q000473:pathology*; D007013:Hypopharynx / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009055:Mouth / Q000473:pathology*; D009062:Mouth Neoplasms / Q000235:genetics* / Q000473:pathology*; D009959:Oropharyngeal Neoplasms / Q000235:genetics* / Q000473:pathology*; D009960:Oropharynx / Q000473:pathology*; D016133:Polymerase Chain Reaction; D012189:Retrospective Studies
D066246:ErbB Receptors
Amplificación del oncogén c-erbB1 y contenido celular de ADN en los carcinomas epidermoides de cabeza y cuello.