Antioxidant and glutathione-associated enzymes in Wilms' tumour after chemotherapy.
The present study demonstrates the activities of antioxidant and glutathione-associated enzymes and the level of glutathione in Wilms' tumour (nephroblastoma) samples after chemotherapy (mainly actinomycin D and vincristine). We observed higher activity of superoxide dismutase in Wilms' tumour compared to adjacent morphologically unchanged kidney. On the other hand, in this tumour lower activities of catalase and the glutathione-associated enzymes glutathione synthetase, gamma-glutamyl transpeptidase, glutathione reductase and total glutathione S-transferases (GST) were found. Using isoelectric focusing we separated different forms of GST in tested tissues and revealed lower activities of the basic enzymes in Wilms' tumour, which may be responsible for the decrease of total GST activity. Moreover, we found the acidic isoenzymes to be the predominant class of GST in nephroblastoma. In Wilms' tumours with unfavourable histology a high activity of these isoenzymes together with a high level of GSH were observed. We suggest that these parameters may participate in the known phenomenon of anticancer drug resistance of tumours with unfavourable histology.
Gajewska
J
J
Department of Biochemistry, National Research Institute of Mother and Child, Warsaw, Poland.
Szczypka
M
M
Izbicki
T
T
Klepacka
T
T
Laskowska-Klita
T
T
D000975:Antioxidants / Q000032:analysis*; D002675:Child, Preschool; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism*; D006801:Humans; D007223:Infant; D007527:Isoenzymes / Q000378:metabolism*; D007680:Kidney Neoplasms / Q000378:metabolism*; D009396:Wilms Tumor / Q000378:metabolism*
D000975:Antioxidants; D007527:Isoenzymes; D005979:Glutathione Peroxidase; D005982:Glutathione Transferase; D005978:Glutathione