Singlet oxygen (1 delta g) generation from coproporphyrin in Propionibacterium acnes on irradiation.
Although singlet oxygen has been postulated to be a highly reactive and toxic intermediate, there has been no evidence of considerable generation of singlet oxygen in vivo level except for special cases. In this work, we firstly measured the near-infrared emission spectra corresponding to the O2(1 delta g) --> O2(3 epsilon g-) transition of singlet oxygen of cutaneous Propionibacterium acnes (P. acnes) porphyrin under laser excitation. A comparison of the singlet oxygen production of coproporphyrin, which is produced predominantly from P. acnes, with that of other photosensitizers revealed coproporphyrin to be a highly efficient singlet oxygen generator under ultraviolet light A irradiation on the skin. These results suggest that singlet oxygen can be generated on the skin surface from P. acnes porphyrin under ultraviolet irradiation and induce serious damage to the skin.
Arakane
K
K
Research Laboratory, Kosé Corporation, Tokyo, Japan.
Ryu
A
A
Hayashi
C
C
Masunaga
T
T
Shinmoto
K
K
Mashiko
S
S
Nagano
T
T
Hirobe
M
M
D003306:Coproporphyrins / Q000302:isolation & purification / Q000528:radiation effects*; D006801:Humans; D007700:Kinetics; D007834:Lasers; D010100:Oxygen; D017319:Photosensitizing Agents / Q000528:radiation effects; D011425:Propionibacterium acnes / Q000302:isolation & purification / Q000378:metabolism*; D026082:Singlet Oxygen; D012867:Skin / Q000382:microbiology*; D013055:Spectrophotometry, Infrared; D014466:Ultraviolet Rays
D003306:Coproporphyrins; D017319:Photosensitizing Agents; D026082:Singlet Oxygen; D010100:Oxygen