title: Zone-specific cell biosynthetic activity in mature bovine articular cartilage: a new method using confocal microscopic stereology and quantitative autoradiography.
abstract: A new methodology was developed to measure spatial variations in chondrocyte/matrix structural parameters and chondrocyte biosynthetic activity in articular cartilage. This technique is based on the use of a laser scanning confocal microscope that can "optically" section chemically fixed, unembedded tissue. The confocal images are used for morphometric measurement of stereologic parameters such as cell density (cells/mm3), cell volume fraction (%), surface density (l/cm), mean cell volume (micron3), and mean cell surface area (micron2). Adjacent pieces of tissue are simultaneously processed for conventional liquid emulsion autoradiography, and a semiautomated grain counting program is used to measure the silver grain density at regions corresponding to the same sites used for structural measurements. An estimate of chondrocyte biosynthetic activity in terms of grains per cell is obtained by dividing the value for grain density by that for cell density. In this paper, the newly developed methodology was applied to characterize the zone-specific behavior of adult articular cartilage in the free-swelling state. Cylinders of young adult bovine articular cartilage were labelled with either [3H]proline or [35S]sulfate, and chondrocyte biosynthesis and structural parameters were measured from the articular surface to the tidemark. The results showed that chondrocytes of the radial zone occupied twice the volume and surface area of the chondrocytes of the superficial zone but were 10 times more synthetically active. This efficient and unbiased technique may prove useful in studying the correlation between mechanically induced changes in cell form and biosynthetic activity within inhomogeneous tissue as well as metabolic changes in cartilage due to ageing and disease.
authors.affiliation: M. E. Mueller Institute for Biomechanics, University of Bern, Switzerland.
authors.forename: M
authors.initials: M
authors.lastname: Wong
authors.forename: P
authors.initials: P
authors.lastname: Wuethrich
authors.forename: P
authors.initials: P
authors.lastname: Eggli
authors.forename: E
authors.initials: E
authors.lastname: Hunziker
mesh_terms: D000818:Animals; D001345:Autoradiography / Q000379:methods*; D002358:Cartilage, Articular / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure; D002417:Cattle; D005260:Female; D018613:Microscopy, Confocal / Q000379:methods*