title: Linear correlation between bacterial overexpression of recombinant peptides and cell light scatter.
abstract: Fusion of multiple copies of a test peptide leads to insoluble inclusion bodies. Their presence within bacteria increases either forward-angle light scattering or, to a lesser extent, right-angle light scattering. A linear correlation has been established between cell forward-angle scattering and the level of overexpression of atrial natriuretic peptide. The correlation is valid only for unlysed cells and is protein product specific.
authors.affiliation: Institut de Biotechnologie, Faculté des Sciences, Limoges, France.
authors.forename: F
authors.initials: F
authors.lastname: Lavergne-Mazeau
authors.forename: A
authors.initials: A
authors.lastname: Maftah
authors.forename: Y
authors.initials: Y
authors.lastname: Cenatiempo
authors.forename: R
authors.initials: R
authors.lastname: Julien
mesh_terms: D009320:Atrial Natriuretic Factor / Q000096:biosynthesis*; D004926:Escherichia coli / Q000235:genetics / Q000502:physiology*; D008027:Light; D011994:Recombinant Proteins / Q000096:biosynthesis*; D012542:Scattering, Radiation
chemical_list: D011994:Recombinant Proteins; D009320:Atrial Natriuretic Factor