Significance of anti-nuclear matrix antibodies in patients with in vivo speckled antinuclear antibody staining.
OBJECTIVE
Evidence suggests that patients with in vivo speckled antinuclear antibody (ANA) patterns have high titers of circulating ANA, specifically anti-U1 RNP antibody. A small percentage of patients with high titers of anti-U1 RNP antibody have anti-nuclear matrix antibodies, and some also demonstrate in vivo ANA. This study was designed to screen for the presence of anti-nuclear matrix antibodies in patients with in vivo ANA.


METHODS
Anti-nuclear matrix antibodies were detected by indirect immunofluorescence on HCI-extracted HEp-2 cell substrate, by enzyme-linked immunosorbent assay, and by immunoblot analysis.


RESULTS
All 10 patients with in vivo ANA were found to have anti-nuclear matrix antibody demonstrated using HCI-extracted HEp-2 cell substrate, and all exhibited antibody activity to a 36-kd protein from nuclear matrix antigen.


CONCLUSIONS
These results suggest that anti-nuclear matrix antibodies are a major factor in the development of in vivo ANA.
Deng
J S
JS
University of Pittsburgh, Pennsylvania, USA.
Benedict
L
L
Deng
D H
DH
Oddis
C V
CV
Fratto
J
J
D000974:Antibodies, Antinuclear / Q000032:analysis* / Q000097:blood / Q000302:isolation & purification; D000918:Antibody Specificity; D034961:Antigens, Nuclear; D001324:Autoantigens / Q000276:immunology* / Q000302:isolation & purification; D002467:Cell Nucleus / Q000737:chemistry / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D015151:Immunoblotting; D015603:Keratinocytes / Q000737:chemistry / Q000276:immunology; D009687:Nuclear Proteins / Q000276:immunology* / Q000302:isolation & purification; D012216:Rheumatic Diseases / Q000276:immunology*; D013194:Staining and Labeling
D000974:Antibodies, Antinuclear; D034961:Antigens, Nuclear; D001324:Autoantigens; D009687:Nuclear Proteins