Microcalorimetric study of mitochondria isolated from fish liver tissue.
We determined the thermogenesis curves of mitochondria isolated from fish liver tissue by using an LKB 2277 Bioactivity Monitor. After isolation from the fish liver, mitochondria still have activity and can live for a long time by using the stored nutrients. We calculated the recovery rate constants of mitochondria. We found that the thermogenesis curves of mitochondria are similar to those obtained from prokaryotic cells, but not similar to those obtained from eukaryotic cells. We determined the metabolic thermogenesis curves of mitochondria isolated from two kinds of carp liver tissue, scattered-scaled mirror carp and harvest carp. There are some important similarities and some important differences between these thermogenesis curves.
Tan
A M
AM
Department of Chemistry, Wuhan University, People's Republic of China.
Xie
C L
CL
Qu
S S
SS
D000818:Animals; D002151:Calorimetry / Q000379:methods*; D002347:Carps; D002458:Cell Fractionation / Q000379:methods; D002498:Centrifugation / Q000379:methods; D007700:Kinetics; D008930:Mitochondria, Liver / Q000378:metabolism*; D012680:Sensitivity and Specificity