title: Cytochrome f encoded by the chloroplast genome is imported into thylakoids via the SecA-dependent pathway.
abstract: In vitro import of the precursor of tobacco cytochrome f, which is is encoded by the chloroplast genome, into isolated pea thylakoids was analyzed. Upon incubation with thylakoids and a stromal fraction, the precursor of cytochrome f was efficiently imported into thylakoids. The imported cytochrome f was tightly integrated into the thylakoid membrane. Insertion of cytochrome f into the thylakoid membrane was blocked by nigericin, sodium azide, and antibodies against pea chloroplast SecA. These results suggest that cytochrome f utilizes the bacterial-type SecA-dependent pathway to be imported into thylakoids.
authors.affiliation: Department of Chemistry, Faculty of Science, Nagoya University, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Nohara
authors.forename: T
authors.initials: T
authors.lastname: Asai
authors.forename: M
authors.initials: M
authors.lastname: Nakai
authors.forename: M
authors.initials: M
authors.lastname: Sugiura
authors.forename: T
authors.initials: T
authors.lastname: Endo
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism*; D001386:Azides / Q000494:pharmacology; D001426:Bacterial Proteins / Q000378:metabolism*; D001692:Biological Transport; D002736:Chloroplasts / Q000378:metabolism*; D003580:Cytochromes / Q000096:biosynthesis / Q000378:metabolism*; D045348:Cytochromes f; D029968:Escherichia coli Proteins; D017343:Genes, Plant; D018745:Genome, Plant; D026901:Membrane Transport Proteins; D009550:Nigericin / Q000494:pharmacology; D015388:Organelles / Q000378:metabolism*; D010947:Plants, Toxic; D014176:Protein Biosynthesis; D011498:Protein Precursors / Q000378:metabolism; D011499:Protein Processing, Post-Translational / Q000187:drug effects; D000069816:SEC Translocation Channels; D000081416:SecA Proteins; D019810:Sodium Azide; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D001386:Azides; D001426:Bacterial Proteins; D003580:Cytochromes; D029968:Escherichia coli Proteins; D026901:Membrane Transport Proteins; D011498:Protein Precursors; D000069816:SEC Translocation Channels; D045348:Cytochromes f; D019810:Sodium Azide; D000251:Adenosine Triphosphatases; D000081416:SecA Proteins; D009550:Nigericin