Different adhesion types and active sensitivity of platelet subpopulations.
Two different adhesion patterns of blood platelets with different sensitivity to adenosine were demonstrated by means of reflection contrast microscopy with consecutive image analysis and cell affinity chromatography. High-performance liquid chromatography revealed that thrombin-induced serotonin release of adenosine-sensitive platelets was lower than that of adenosine-resistant cells. Our results indicate platelet heterogeneity and suggest that the platelets with lower adenosine sensitivity may be actively involved in the early interaction between platelets and injured endothelium.
Zhao
B
B
Platelet Research and Image Analysis Unit, Institute of Anatomy, University of Münster, Germany.
Filler
T J
TJ
Rickert
C H
CH
Liu
J F
JF
Skholl-Engberts
A D
AD
Gurevich
V
V
D000241:Adenosine / Q000494:pharmacology*; D001792:Blood Platelets / Q000187:drug effects / Q000502:physiology; D002851:Chromatography, High Pressure Liquid; D004351:Drug Resistance; D006801:Humans; D010973:Platelet Adhesiveness / Q000187:drug effects*; D012701:Serotonin / Q000378:metabolism; D013917:Thrombin / Q000494:pharmacology
D012701:Serotonin; D013917:Thrombin; D000241:Adenosine