Inhibition by verapamil of cholecystokinin-enhancement of pancreatic carcinogenesis induced by azaserine in Wistar rats.
The effect of a calcium channel blocker, verapamil, on cholecystokinin (CCK)-enhancement of pancreatic carcinogenesis induced by azaserine was investigated in Wistar rats. During and after 25 weekly injections of azaserine, each rat received alternate-day injections of CCK-octapeptide (CCK-8) and/or verapamil. Carcinogen-induced pancreatic lesions staining for mu class glutathione S-transferase (GST-mu) were examined histochemically at week 62. Prolonged administration of CCK-8 significantly increased the number and area as a percentage of parenchyma of GST-mu-positive lesions. Concomitant administration of verapamil significantly attenuated the enhancing effect of CCK-8. These findings indicate that calcium may play an important role in CCK-enhancement of pancreatic carcinogenesis.
Nakaizumi
A
A
Department of Gastrointestinal Oncology, Osaka Medical Center for Cancer and Cardiovascular Diseases, Japan.
Uehara
H
H
Baba
M
M
Iishi
H
H
Tatsuta
M
M
D000818:Animals; D001377:Azaserine / Q000008:administration & dosage*; D002121:Calcium Channel Blockers / Q000494:pharmacology; D002766:Cholecystokinin / Q000008:administration & dosage*; D004357:Drug Synergism; D005982:Glutathione Transferase / Q000378:metabolism; D008297:Male; D010190:Pancreatic Neoplasms / Q000139:chemically induced*; D051381:Rats; D017208:Rats, Wistar; D014700:Verapamil / Q000008:administration & dosage*
D002121:Calcium Channel Blockers; D001377:Azaserine; D002766:Cholecystokinin; D014700:Verapamil; D005982:Glutathione Transferase