title: Identification of a fibronectin-binding protein (GfbA) in pathogenic group G streptococci.
abstract: Attachment to eukaryotic cell surfaces is an essential step in the establishment of colonization and infection by bacterial pathogens. This report examines the adherence capabilities of pathogenic group G streptococci and demonstrates that certain group G streptococcal clinical isolates express a fibronectin-binding protein. This protein, termed GfbA for group G streptococcal fibronectin-binding protein, mediates adherence to human skin fibroblasts (HSF). The gene encoding this protein, gfbA, was isolated, and the complete DNA sequence of gfbA was determined. From this sequence GfbA was predicted to be a 580-amino-acid protein (molecular weight = 64,979) with significant amino acid identity to the group A streptococcal fibronectin-binding proteins SfbI and protein F (PrtF) (76 and 78% identity, respectively). GfbA contains regions with notable identity to the fibronectin-binding repeat domains of PrtF. gfbA(+) strains were able to bind to HSF, and preincubation of the gfbA(+) strains with fibronectin blocked this adherence. In addition, gfbA(+) strains were able to bind radiolabeled fibronectin, and this binding was inhibited with addition of excess unlabeled fibronectin. gfbA-negative strains were not able to bind either the HSF or radiolabeled fibronectin. DNA homologous to gfbA was found in 36% of the group G streptococcal isolates examined. Since not all group G streptococcal strains examined contained gfbA, this suggests there might be other tissue-specific adherence molecules expressed by these pathogenic strains.
authors.affiliation: Department of Microbiology, University of Miami School of Medicine, Florida 33101, USA.
authors.forename: J B
authors.initials: JB
authors.lastname: Kline
authors.forename: S
authors.initials: S
authors.lastname: Xu
authors.forename: A L
authors.initials: AL
authors.lastname: Bisno
authors.forename: C M
authors.initials: CM
authors.lastname: Collins
mesh_terms: D018829:Adhesins, Bacterial; D000595:Amino Acid Sequence; D001422:Bacterial Adhesion; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000302:isolation & purification*; D001426:Bacterial Proteins; D001483:Base Sequence; D002352:Carrier Proteins; D005353:Fibronectins / Q000378:metabolism; D005798:Genes, Bacterial; D006801:Humans; D008969:Molecular Sequence Data; D013291:Streptococcus / Q000737:chemistry* / Q000235:genetics / Q000502:physiology
chemical_list: D018829:Adhesins, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D002352:Carrier Proteins; D005353:Fibronectins; C078635:fibronectin-binding proteins, bacterial