title: Phenotypic analysis of mice expressing exclusively apolipoprotein B48 or apolipoprotein B100.
abstract: Apolipoprotein (apo)-B is found in two forms in mammals: apo-B100, which is made in the liver and the yolk sac, and apo-B48, a truncated protein made in the intestine. To provide models for understanding the physiologic purpose for the two forms of apo-B, we used targeted mutagenesis of the apo-B gene to generate mice that synthesize exclusively apo-B48 (apo-B48-only mice) and mice that synthesize exclusively apo-B100 (apo-B100-only mice). Both the apo-B48-only mice and apo-B100-only mice developed normally, were healthy, and were fertile. Thus, apo-B48 synthesis was sufficient for normal embryonic development, and the synthesis of apo-B100 in the intestines of adult mice caused no readily apparent adverse effects on intestinal function or nutrition. Compared with wild-type mice fed a chow diet, the levels of low density lipoprotein (LDL)-cholesterol and very low density lipoprotein- and LDL-triacylglycerols were lower in apo-B48-only mice and higher in the apo-B100-only mice. In the setting of apo-E-deficiency, the apo-B100-only mutation lowered cholesterol levels, consistent with the fact that apo-B100-lipoproteins can be cleared from the plasma via the LDL receptor, whereas apo-B48-lipoproteins lacking apo-E cannot. The apo-B48-only and apo-B100-only mice should prove to be valuable models for experiments designed to understand the purpose for the two forms of apo-B in mammalian metabolism.
authors.affiliation: Gladstone Institute of Cardiovascular Disease, San Francisco, CA 94141-9100, USA.
authors.forename: R V
authors.initials: RV
authors.lastname: Farese
authors.forename: M M
authors.initials: MM
authors.lastname: Véniant
authors.forename: C M
authors.initials: CM
authors.lastname: Cham
authors.forename: L M
authors.initials: LM
authors.lastname: Flynn
authors.forename: V
authors.initials: V
authors.lastname: Pierotti
authors.forename: J F
authors.initials: JF
authors.lastname: Loring
authors.forename: M
authors.initials: M
authors.lastname: Traber
authors.forename: S
authors.initials: S
authors.lastname: Ruland
authors.forename: R S
authors.initials: RS
authors.lastname: Stokowski
authors.forename: D
authors.initials: D
authors.lastname: Huszar
authors.forename: S G
authors.initials: SG
authors.lastname: Young
mesh_terms: D000818:Animals; D053299:Apolipoprotein B-100; D053283:Apolipoprotein B-48; D001055:Apolipoproteins B / Q000235:genetics*; D001483:Base Sequence; D008078:Cholesterol, LDL / Q000097:blood; D015243:Cholesterol, VLDL / Q000097:blood; D004041:Dietary Fats / Q000008:administration & dosage; D005260:Female; D051379:Mice; D008969:Molecular Sequence Data; D010641:Phenotype; D014280:Triglycerides / Q000097:blood
chemical_list: D053299:Apolipoprotein B-100; D053283:Apolipoprotein B-48; D001055:Apolipoproteins B; D008078:Cholesterol, LDL; D015243:Cholesterol, VLDL; D004041:Dietary Fats; D014280:Triglycerides