A general assay for restriction endonucleases and other DNA-modifying enzymes with plasmid substrates.
A procedure for measuring the activities of enzymes that alter the covalent structure of DNA is described. The assay utilizes covalently closed circles of DNA as the substrate and yields quantitative data on the fraction of this DNA converted to both open-circle and linear forms.
Vipond
I B
IB
Department of Biochemistry, Centre for Molecular Recognition, University of Bristol, UK.
Baldwin
G S
GS
Oram
M
M
Erskine
S G
SG
Wentzell
L M
LM
Szczelkun
M D
MD
Nobbs
T J
TJ
Halford
S E
SE
D010582:Bacteriophage lambda / Q000235:genetics; D004254:DNA Nucleotidyltransferases / Q000032:analysis / Q000378:metabolism; D004262:DNA Restriction Enzymes / Q000032:analysis* / Q000378:metabolism; D004270:DNA, Circular / Q000737:chemistry; D015246:Deoxyribonuclease EcoRI / Q000502:physiology; D004587:Electrophoresis, Agar Gel; D009691:Nucleic Acid Denaturation; D010957:Plasmids / Q000235:genetics; D011995:Recombination, Genetic; D019895:Transposases
D004270:DNA, Circular; D004254:DNA Nucleotidyltransferases; D019895:Transposases; D004262:DNA Restriction Enzymes; D015246:Deoxyribonuclease EcoRI