The role of transforming growth factor beta in Dupuytren's disease.
This study was undertaken to mark immunologically intracellular and extracellular sites of two common transforming growth factor beta (TGF-beta) isoforms, TGF-beta1 and TGF-beta2, in the proliferative, involutional, and residual stages of Dupuytren's disease. The effect of TGF-beta on myofibroblast proliferation was also studied using explant cultures from Dupuytren's nodules in the proliferative or involutional stage. TGF-beta1, TGF-beta2 and the combination of both isoforms were studied at low and high myofibroblast plating densities to simulate respectively proliferative or involutional disease stage conditions. Our results indicate that TGF-beta1 showed an intense intracellular marking pattern associated with fibroblasts, myofibroblasts, and capillary endothelial cells in all Dupuytren's samples, regardless of disease stage. TGF-beta2 showed an intense intracellular localization within myofibroblasts in the proliferative and involutional stages. Fibroblasts in the residual stage did not contain TGF-beta2. Neither isoform was present in the extracellular matrix. Results of cell culture indicate that compared with control myofibroblasts, the addition of TGF-beta1, TGF-beta2 and TGF-beta1 + beta2 had significant effects on myofibroblast proliferation, especially at higher plating densities. However, TGF-beta2 had the most significant proliferative effect.
Badalamente
M A
MA
Department of Orthopaedics, State University of New York at Stony Brook 11794, USA.
Sampson
S P
SP
Hurst
L C
LC
Dowd
A
A
Miyasaka
K
K
D002455:Cell Division / Q000502:physiology*; D002478:Cells, Cultured; D004387:Dupuytren Contracture / Q000473:pathology* / Q000601:surgery; D005205:Fascia / Q000473:pathology; D000071938:Fasciotomy; D005260:Female; D005347:Fibroblasts / Q000473:pathology; D006227:Hand Deformities, Acquired / Q000473:pathology* / Q000601:surgery; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007124:Immunoenzyme Techniques; D008297:Male; D008875:Middle Aged; D016212:Transforming Growth Factor beta / Q000032:analysis*
D016212:Transforming Growth Factor beta