title: [K-ras gene mutations in adenomas from familial adenomatous polyposis].
abstract: A 18-year-old woman underwent total colectomy for familial adenomatous polyposis. In order to clarify the significance of K-ras mutations in early colorectal carcinogenesis, K-ras mutations were analyzed in multiple adenomas by PCR-SSCP method. A total of 256 adenomas were found throughout the entire colon and rectum, and the distribution was a sparse type. The correlation between K-ras gene and clinicopathological factors was examined in 90 adenomas. There was no correlation among K-ras mutations and anatomical distribution, or morphological classification, but K-ras mutation was more frequent in severe compared with slight atypia. We investigated the correlation between the size of adenoma in the horizontal and vertical directions and K-ras mutation. K-ras mutation was more frequent in the horizontal size greater than 6 mm in diameter, and also more frequent in vertical size greater than 20 mm in height. It was concluded that the adenomas detecting K-ras mutations might have proliferating potential, and would be applied to determine polypectomy.
authors.affiliation: Second Dept. of Surgery, Fukushima Medical College, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Ohki
authors.forename: M
authors.initials: M
authors.lastname: Onda
authors.forename: T
authors.initials: T
authors.lastname: Nomizu
authors.forename: A
authors.initials: A
authors.lastname: Tsuchiya
authors.forename: H
authors.initials: H
authors.lastname: Ami
authors.forename: T
authors.initials: T
authors.lastname: Fukushima
authors.forename: K
authors.initials: K
authors.lastname: Takita
authors.forename: M
authors.initials: M
authors.lastname: Kanazawa
authors.forename: F
authors.initials: F
authors.lastname: Watanabe
authors.forename: Y
authors.initials: Y
authors.lastname: Andoh
authors.forename: T
authors.initials: T
authors.lastname: Yoshida
authors.forename: R
authors.initials: R
authors.lastname: Abe
mesh_terms: D000236:Adenoma / Q000235:genetics* / Q000473:pathology; D011125:Adenomatous Polyposis Coli / Q000235:genetics* / Q000473:pathology; D003111:Colonic Polyps / Q000235:genetics; D005260:Female; D017491:Genes, APC; D011905:Genes, ras / Q000235:genetics*; D006801:Humans; D008297:Male; D009154:Mutation; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational