Using SAR and QSAR analysis to model the activity and structure of the quinolone-DNA complex.
A set of 78 quinolone derivatives were used in a structure-activity study to identify structural features correlating with antibacterial activity. Distinct combinations of functional properties were identified for Gram-negative and Gram-positive bacteria. 3-D Quantitative structure-activity relationship (QSAR) studies identified specific hydrophobic, topologic and electronic properties of the molecules for both in vitro and in vivo activities. From these results, a three-dimensional model of a DNA-quinolone complex was built using molecular modeling techniques. It was based on the intercalation of quinolone into the double helix of DNA. We conclude that the intercalation model is consistent with most available data on the structure of the quinolone complex. This predicted structure is stabilized by the binding of magnesium ion with the sp2 oxygens present in quinolone, a phosphate and a purine base of the DNA. Substituents R1 and R7 are predicted to make hydrophobic interactions in the major and minor groove of DNA, respectively. R7 could also form hydrogen bonds with amino groups of guanines and the aspartic acid residue at position 87 in DNA gyrase subunit A.
Llorente
B
B
Départment de Biochimie, Université de Montréal, Canada.
Leclerc
F
F
Cedergren
R
R
D004250:DNA Topoisomerases, Type II / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry* / Q000378:metabolism; D008954:Models, Biological; D008958:Models, Molecular; D015363:Quinolones / Q000737:chemistry* / Q000378:metabolism; D013329:Structure-Activity Relationship
D004269:DNA, Bacterial; D015363:Quinolones; D004250:DNA Topoisomerases, Type II