title: Developmental heterogeneity of V gamma 1.1 T cells in the mouse liver.
abstract: Modifications at V-(D)-J junctions increase the diversity of T-cell receptors (TCR). It has been shown that the levels of N-nucleotide insertion at the V-(D)-J junction in TCR transcripts are different between fetal and adult stages. To clarify developmental stages and pathways of gamma delta T cells in the liver, we analysed the nucleotide sequence of V gamma 1.1-J gamma 4 junctions of intra-hepatic lymphocytes (IHL), spleen cells and developing thymocytes from normal and athymic nude mice. The level of N-insertion increased in thymocytes during ontogeny. The percentage of V gamma 1.1-J gamma 4 transcripts with N-insertion was 3% at day 16 of gestation, 42% at newborn, and 89% at 7 weeks. Transcripts from normal IHL showed intermediate levels of N-insertion between those of newborn and adult thymocytes. In contrast the percentage of N-insertion in nude IHL was 47%, and this value was comparable to that of newborn thymocytes. Among the transcripts of normal IHL, the sequences common with nude IHL showed a newborn level of N-insertion (38%), and the remaining sequences showed an adult level (89%). These results suggested the possibility that V gamma 1.1-expressing T cells in IHL might be a heterogeneous population consisting of the cells developed extrathymically as well as the cells developed intrathymically. The V gamma 1.1-J gamma 4 junctions from spleen cells showed less variability than those from IHL and adult thymocytes. It suggested that gamma delta T cells bearing specific V gamma 1.1 TCR develop and/or home in the spleen.
authors.affiliation: Department of Disease-Related Gene Regulation Research (Sandoz), University of Tokyo, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Kodaira
authors.forename: K
authors.initials: K
authors.lastname: Yokomuro
authors.forename: S
authors.initials: S
authors.lastname: Tanaka
authors.forename: J I
authors.initials: JI
authors.lastname: Miyazaki
authors.forename: K
authors.initials: K
authors.lastname: Ikuta
mesh_terms: D000367:Age Factors; D000818:Animals; D001483:Base Sequence; D004253:DNA Nucleotidylexotransferase / Q000235:genetics; D007111:Immunity, Cellular; D007133:Immunoglobulin Joining Region / Q000235:genetics; D007135:Immunoglobulin Variable Region / Q000235:genetics; D008099:Liver / Q000166:cytology / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000235:genetics* / Q000378:metabolism; D013154:Spleen / Q000276:immunology; D013950:Thymus Gland / Q000196:embryology / Q000254:growth & development / Q000276:immunology
chemical_list: D007133:Immunoglobulin Joining Region; D007135:Immunoglobulin Variable Region; D012333:RNA, Messenger; D016692:Receptors, Antigen, T-Cell, gamma-delta; D004253:DNA Nucleotidylexotransferase