Localization of mRNA encoding c-kit during the initiation of folliculogenesis in ovine fetal ovaries.
The c-kit protein is a transmembrane tyrosine kinase receptor that binds the growth factor stem cell factor. Mutant alleles of the genes coding for both the receptor (c-kit) and its ligand (stem cell factor) affect gametogenesis, development of melanoblasts and some aspects of haematopoiesis. The aim of this study was to examine expression of the c-kit gene during folliculogenesis in fetal sheep ovaries using in situ hybridization. A 422 bp cDNA encoding the extracellular domain of the c-kit protein was amplified from sheep ovarian RNA using reverse-transcription-polymerase chain reaction (RT-PCR), cloned and sequenced. Riboprobes transcribed from the ovine cDNA encoding c-kit were used to detect the presence of mRNA encoding c-kit within the ovaries of fetal sheep on days 90, 100, 120 and 135 of gestation (term = 147 days). In day 90 and 100 fetal ovaries, mRNA encoding c-kit was not detected in association with oogonia during the period of meiosis (to prophase I) but was present in some of the isolated oocytes. In ovaries from day 90 to day 135, mRNA encoding c-kit was detected in the oocytes at every stage of follicular growth--primordial through to antral follicles. This pattern of localization is consistent with that demonstrated in mice.
Clark
D E
DE
AgResearch, Wallaceville Animal Research Centre, Upper Hutt, New Zealand.
Tisdall
D J
DJ
Fidler
A E
AE
McNatty
K P
KP
D000818:Animals; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D005260:Female; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D006080:Ovarian Follicle / Q000502:physiology*; D010053:Ovary / Q000737:chemistry / Q000196:embryology*; D016133:Polymerase Chain Reaction; D019009:Proto-Oncogene Proteins c-kit / Q000032:analysis / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis*; D012756:Sheep / Q000196:embryology*
D017931:DNA Primers; D012333:RNA, Messenger; D019009:Proto-Oncogene Proteins c-kit