Detection of immunoglobulin G to a Sindbis-related virus by a membrane antigen enzyme immunoassay.
We determined the seroprevalence of a Sindbis-related virus isolated for the first time in 1975 from ticks in south-east Sicily and typed by Gresikova et al. in 1978. An indirect enzyme immunoassay based on viral membrane antigen for coating microtiter strips was used for the detection of immunoglobulin G to the Sindbis-related virus. The method appeared more sensitive than a similar enzyme immunoassay based on crude lysate antigen. Comparison of the results obtained from sera tested both by membrane antigen enzyme immunoassay and microneutralization test showed 92% agreement, while the agreement between microneutralization test and crude antigen enzyme immunoassay was 76%. An overall elevated seroprevalence (63.66%) was found in a population group living in and around the area of first isolation and seroprevalence in different age groups was also studied.
Scalia
G
G
Institute of Microbiology, University of Catania, Italy.
Garufi
M
M
Condorelli
F
F
Stivala
A
A
Costanzo
M C
MC
Tiralongo
M
M
Adragna
A D
AD
Marino
A
A
Guglielmino
S
S
Tempera
G
G
Castro
A
A
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D018354:Alphavirus Infections / Q000175:diagnosis* / Q000453:epidemiology; D000914:Antibodies, Viral / Q000097:blood*; D000956:Antigens, Viral / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D006801:Humans; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000097:blood; D007223:Infant; D008875:Middle Aged; D009500:Neutralization Tests; D011934:Reagent Strips; D012802:Sicily / Q000453:epidemiology; D012845:Sindbis Virus / Q000276:immunology*; D014763:Viral Matrix Proteins / Q000276:immunology
D000914:Antibodies, Viral; D000956:Antigens, Viral; D007074:Immunoglobulin G; D011934:Reagent Strips; D014763:Viral Matrix Proteins