Characterization of Toronto virus capsid protein expressed in baculovirus.
Toronto virus (TV), previously called "minireovirus", a human calicivirus classified as genogroup 2 and phylogenetic type P2-A, was originally described in association with diarrhea in children. The second open reading frame, encoding the capsid protein of TV24, was expressed in a baculovirus recombinant. The recombinant baculovirus produced a protein (rTV) with an apparent molecular mass of 58 kDa that self-assembled into virus-like particles approximately 30 nm in diameter with a density of 1.29 g/ml. Antigenic and immunogenic characteristics of these particles were determined by protein immunoblot, immunoprecipitation, and enzyme immunoassay. Seroconversion to the rTV protein was detected in 6 of 8 (75%) patients from a recent outbreak of gastroenteritis associated with a virus of similar phylogenetic type. These results confirm and extend the previous reports of the expression of the Norwalk and Mexico virus capsid proteins.
Leite
J P
JP
Viral Gastroenteritis Section, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
Ando
T
T
Noel
J S
JS
Jiang
B
B
Humphrey
C D
CD
Lew
J F
JF
Green
K Y
KY
Glass
R I
RI
Monroe
S S
SS
D000818:Animals; D016367:Baculoviridae / Q000235:genetics; D001483:Base Sequence; D002139:Caliciviridae / Q000737:chemistry*; D002213:Capsid / Q000096:biosynthesis* / Q000276:immunology / Q000302:isolation & purification; D008969:Molecular Sequence Data; D011817:Rabbits; D011994:Recombinant Proteins / Q000096:biosynthesis; D018411:Spodoptera; D019065:Virus Assembly
D011994:Recombinant Proteins