Transport of glutathione S-conjugates in the yeasts Saccharomyces cerevisiae.
Transport of 2,4-dinitrophenyl-S-glutathione (DNP-SG) and a fluorescent glutathione S-conjugate, bimane-S-glutathione (B-SG) was studied in the baker's yeasts (S. cerevisiae). Both conjugates were exported from the cells; the transport was inhibited by fluoride and vanadate like in mammalian cells. B-SG was also found to be accumulated in the vacuoles. The transport rate of DNP-SG outside the cell was higher in a vacuolar-deficient strain. A significant ATP-dependent uptake of (3H)-DNP-SG by vacuoles was found. These results indicate that S. cerevisiae transport glutathione S-conjugates both outside the cells and into the vacuoles.
Zadziński
R
R
Department of Molecular Biophysics, University of Lódź, Poland.
Maszewski
J
J
Bartosz
G
G
D001692:Biological Transport / Q000502:physiology; D001643:Bridged Bicyclo Compounds / Q000493:pharmacokinetics; D002352:Carrier Proteins / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000493:pharmacokinetics; D005978:Glutathione / Q000031:analogs & derivatives / Q000493:pharmacokinetics; D006241:Haptens / Q000378:metabolism; D026901:Membrane Transport Proteins; D008856:Microscopy, Fluorescence; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D014316:Tritium
D001643:Bridged Bicyclo Compounds; D002352:Carrier Proteins; D005456:Fluorescent Dyes; D006241:Haptens; D026901:Membrane Transport Proteins; C086860:glutathione transporter; C080120:glutathione-bimane; D014316:Tritium; C030190:S-(2,4-dinitrophenyl)glutathione; D005978:Glutathione