Direct quantification of HIV-1 RNA in human plasma by free solution capillary electrophoresis (FSCE).
The levels of human immunodeficiency virus type 1 (HIV-1) RNA have been directly quantitated, after an isolation step, in plasma from patients with primary HIV-1 infection by free solution capillary electrophoresis (FSCE) with ultraviolet detection. HIV-1 RNA was detected and quantified at physiological levels by measuring the absorbance by FSCE. All the patients with primary infection showed concentrations in a range of 1.08-1.71 x 10(8) virions/ml of plasma. No signals were observed in seronegative donors. This procedure represents a practical alternative to other methods to quantify HIV-1 RNA and may be useful in assessing the efficiency of antiretroviral agents, especially during the early stage when other conventional viral markers are often negative.
Fernández-Arcás
N
N
Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Málaga, Spain.
Dieguez-Lucena
J L
JL
García-Villanova
J G
JG
Peña
J
J
Morell-Ocaña
M
M
Reyes-Engel
A
A
D016022:Case-Control Studies; D019075:Electrophoresis, Capillary / Q000379:methods* / Q000592:standards / Q000706:statistics & numerical data; D005069:Evaluation Studies as Topic; D015658:HIV Infections / Q000821:virology*; D018023:HIV Seronegativity; D015497:HIV-1 / Q000302:isolation & purification* / Q000502:physiology; D006801:Humans; D012367:RNA, Viral / Q000097:blood* / Q000592:standards; D012015:Reference Standards; D015203:Reproducibility of Results; D014779:Virus Replication
D012367:RNA, Viral