Purification and characterization of multiple forms of odorant/pheromone binding proteins in the antennae of Mamestra brassicae (Noctuidae).
Proteins extracted form the antennae of Mamestra brassicae (L.) (Lepidoptera: Noctuidae) adults were biochemically characterized as pheromone-binding proteins (PBP) and general odorant-binding proteins (GOBP). PBP and GOBP were purified by two successive and different HPLC (high performance liquid chromatography) systems and native polyacrylamide gel electrophoresis (native-PAGE). Their N-terminal sequence was determined by Edman microsequencing. The combined results showed evidence for three different PBPs in males, and two different PBPs in females. In addition, one GOBP was characterized in both males than in females antennae. In the males, two isoforms of PBP have the same N-terminal sequence, but different apparent mobilities and hydrophobicities: they could be separated by electrophoresis and reverse phase-HPLC (RP-HPLC). The other PBP sequence (SQEIM) showed particularly high homology (88%) with the PBP of Heliothis virescens, another noctuid moth. The existence of several forms of PBP in the same animal strongly supports the hypothesis of the specificity of binding between the proteins and their odorant ligands, the pheromonal compounds. The observed microdiversity at the soluble proteins level could provide a good model for studying their involvement in the initial stages of odor discrimination.
Nagnan-Le Meillour
P
P
Unité de Phytopharmacie et des Médiateurs Chimiques, INRA, Versailles, France.
Huet
J C
JC
Maibeche
M
M
Pernollet
J C
JC
Descoins
C
C
D000595:Amino Acid Sequence; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D007301:Insect Hormones / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D009036:Moths / Q000737:chemistry*; D018035:Receptors, Odorant / Q000737:chemistry* / Q000302:isolation & purification; D017386:Sequence Homology, Amino Acid
D007301:Insect Hormones; D018035:Receptors, Odorant; C045648:odorant-binding protein