title: Motion of spin-labeled side chains in T4 lysozyme. Correlation with protein structure and dynamics.
abstract: Thirty single cysteine substitution mutants of T4 lysozyme have been prepared and spin-labeled with a sulfhydryl-specific nitroxide reagent in order to systematically investigate the relationship between nitroxide side-chain mobility and protein structure. The perturbation caused by replacement of a native residue with a nitroxide amino acid was assessed from the resulting changes in biological activity, circular dichroism, and free energy of folding. The nitroxide produced context-dependent changes in stability and activity similar to those observed for substitution with natural amino acids at the same site but had little effect on the circular dichroism spectra. At solvent-exposed sites, the structural perturbation appears to be small at the level of the backbone fold. Nitroxide side-chain mobility faithfully reflects the protein tertiary fold at all sites investigated. The primary determinants of nitroxide side-chain mobility are tertiary interactions and backbone dynamics. Tertiary interactions constrain the side-chain mobility to an extent closely correlated with the degree of interaction. At interhelical loop sites, the side chains have a high mobility, consistent with high crystallographic thermal factors. On the exposed surfaces of alpha-helices, the side-chain mobility is not restricted by interactions with nearest neighbor side chains but appears to be determined by backbone dynamics. An unexpected result is a striking difference between the mobility of residues near the C- and N-termini of helices. These results provide the foundation for another dimension of information in site-directed spin-labeling experiments that can be interpreted in terms of the protein tertiary fold, its equilibrium dynamics and time-dependent conformational changes.
authors.affiliation: Jules Stein Eye Institute, University of California, Los Angeles 90095-7008, USA.
authors.forename: H S
authors.initials: HS
authors.lastname: Mchaourab
authors.forename: M A
authors.initials: MA
authors.lastname: Lietzow
authors.forename: K
authors.initials: K
authors.lastname: Hideg
authors.forename: W L
authors.initials: WL
authors.lastname: Hubbell
mesh_terms: D017122:Bacteriophage T4 / Q000201:enzymology*; D002942:Circular Dichroism; D003001:Cloning, Molecular; D004578:Electron Spin Resonance Spectroscopy; D004926:Escherichia coli; D007700:Kinetics; D008958:Models, Molecular; D008961:Models, Structural; D009113:Muramidase / Q000737:chemistry* / Q000378:metabolism*; D016297:Mutagenesis, Site-Directed; D010457:Peptidoglycan / Q000378:metabolism; D017354:Point Mutation; D011487:Protein Conformation; D011489:Protein Denaturation; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D013113:Spin Labels; D013439:Sulfhydryl Reagents; D013816:Thermodynamics
chemical_list: D010457:Peptidoglycan; D011994:Recombinant Proteins; D013113:Spin Labels; D013439:Sulfhydryl Reagents; D009113:Muramidase