Lipid peroxide levels and superoxide-scavenging abilities of Sera obtained from hotbred (Thoroughbred) horses.
Hotbred (Thoroughbred) horses were grouped into three classes according to the levels of constant physical exercise (foals, 6 months old; racing horses, 5 years old; horses for breeding, 6-10 years old), and lipid peroxide levels in their sera were measured as thiobarbituric acid-reactive substances. No significant differences were observed among them. The superoxide-scavenging abilities of sera were measured next; to examine the antioxidative properties of hotbreds, and were found to be highest in the racing horses. The higher scavenging ability of the racing horses might contribute to keep their lipid peroxide levels as low as those of the other two groups. HPLC analysis of substances in sera suggested that the presence of albumin-bound bilirubin was one of the reasons for the high superoxide-scavenging ability of sera of the racing horses. When the hotbreds were compared with coldbred (crossbred) horses, the lipid peroxide levels of hotbreds was higher (7.0 +/- 1.2 nmol/ml) than that of coldbreds (2.6 +/- 0.7 nmol/ml). Comparison of the superoxide-scavenging abilities of sera between hotbreds and coldbreds showed that the hotbreds possessed higher scavenging ability than the coldbreds. These results indicated that the lipid peroxide level in sera of hotbreds was higher than that of coldbreds regardless of the higher superoxide-scavenging abilities of sera.
Kuwabara
M
M
Department of Environmental Veterinary Science, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
Inukai
N
N
Inanami
O
O
Miyake
Y I
YI
Tsunoda
N
N
Maki
Y
Y
Sato
F
F
D000818:Animals; D001663:Bilirubin / Q000097:blood; D001947:Breeding; D002851:Chromatography, High Pressure Liquid / Q000662:veterinary; D004578:Electron Spin Resonance Spectroscopy / Q000662:veterinary; D005260:Female; D016166:Free Radical Scavengers / Q000097:blood*; D006736:Horses / Q000097:blood* / Q000235:genetics / Q000502:physiology; D008054:Lipid Peroxides / Q000097:blood*; D008297:Male; D010805:Physical Conditioning, Animal / Q000502:physiology; D013481:Superoxides / Q000378:metabolism*; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis
D016166:Free Radical Scavengers; D008054:Lipid Peroxides; D017392:Thiobarbituric Acid Reactive Substances; D013481:Superoxides; D001663:Bilirubin