Antiperoxidative components in Thymus vulgaris.
A biphenyl compound, 3,4,3',4'-tetrahydroxy-5,5'-diisopropyl-2,2'-dimethylbiphenyl (1), and a flavonoid, eriodicytol (2), were isolated as antioxidative components from the leaves of Thymus vulgaris by bioassay-directed fractionation. These compounds inhibited superoxide anion production in the xanthine/xanthine oxidase system. Mitochondrial and microsomal lipid peroxidation induced by Fe(III)-ADP/NADH or Fe(III)-ADP/NADPH were also inhibited by these compounds. Compound 1 is an extremely potent antioxidant; complete inhibition was observed at 1 microM against both microsomal and mitochondrial peroxidation. Furthermore, compound 1 protected red cells against oxidative hemolysis. These phenolic compounds were shown to be effective to protect biological systems against various oxidative stresses.
Haraguchi
H
H
Faculty of Engineering, Fukuyama University, Japan.
Saito
T
T
Ishikawa
H
H
Date
H
H
Kataoka
S
S
Tamura
Y
Y
Mizutani
K
K
D000818:Animals; D000975:Antioxidants / Q000302:isolation & purification* / Q000494:pharmacology; D006461:Hemolysis; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects*; D019684:Magnoliopsida; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism; D010636:Phenols / Q000302:isolation & purification* / Q000494:pharmacology; D010936:Plant Extracts; D018515:Plant Leaves; D051381:Rats; D017208:Rats, Wistar; D013481:Superoxides / Q000378:metabolism; D019820:Xanthine; D014969:Xanthine Oxidase / Q000378:metabolism; D014970:Xanthines / Q000378:metabolism
D000975:Antioxidants; D010636:Phenols; D010936:Plant Extracts; D014970:Xanthines; D013481:Superoxides; D019820:Xanthine; D014969:Xanthine Oxidase