[The role of oxygen radicals formed during function of the membrane redox chain, in damage of nuclear DNA].
Nuclear DNA damage resulting from incubation of isolated rat liver nuclei in the presence of NADPH or NADH and ethylenediamine tetraacetic acid, or diethylenetriamine pentaacetic acid, or desferoxamine complexes with Fe3+, has been studied. The degree of DNA damage was assessed by agarose electrophoresis. The observed damage was prevented by catalase but not superoxide dismutase. The mechanism of oxidative damage of nuclear DNA during membrane redox chains functioning is discussed.
Peskin
A V
AV
N.K. Kol'tsov Institute of Developmental Biology, Russian Academy of Sciences, Moscow. peskin@ibrran.msk.su
D000818:Animals; D002374:Catalase / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism*; D004249:DNA Damage; D004492:Edetic Acid; D005609:Free Radicals; D008099:Liver / Q000378:metabolism; D009243:NAD / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D051381:Rats; D017382:Reactive Oxygen Species; D013482:Superoxide Dismutase / Q000378:metabolism
D005609:Free Radicals; D017382:Reactive Oxygen Species; D009243:NAD; D009249:NADP; D004492:Edetic Acid; D002374:Catalase; D013482:Superoxide Dismutase
Rol' kislorodnykh radikalov, obrazuiushchikhsia pri funktsionirovanii membrannykh redoks-tsepeĭ, v povrezhdenii iadernoĭ DNK.