A light and scanning electron microscopic evaluation of electro-discharge-compacted porous titanium implants in rabbit tibia.
This study used light and scanning electron microscopic (SEM) histomorphometric methods to quantitate the rate of osseointegration of totally porous titanium alloy (Ti-6Al-4V) implants prepared by a novel fabrication technique--electrodischarge compaction (EDC). EDC was used to fuse 150-250-micrometer spherical titanium alloy beads into 4 X 6 mm cylindrical implants through application of a 300-microsecond pulse of high-voltage/high-current density. Two sterilized implants were surgically placed into each tibia of 20 New Zealand white rabbits and left in situ for periods corresponding to 2, 4, 8, 12, and 24 weeks. At each time point, 4 rabbits were humanely killed, and the implants with surrounding bone were removed, fixed, and sectioned for light and SEM studies. The degree of osseointegration was quantitated by means of a True Grid Digitizing Pad and Jandel Scan Version 3.9 software on an IBM PS/2 computer. The total pore area occupied by bone was divided by the total pore area available for bone ingrowth, and a Bone Ingrowth Factor (BIF) was calculated as a percent. The light microscopic results showed BIFs of 4% at week 2, 47% at week 4, 62% at week 8, 84% at week 12, and greater than 90% at week 24. The SEM results showed BIFs of 5% at week 2, 34% at week 4, 69% at week 8, 75% at week 12, and in excess of 90% at week 24. The results of this study show that EDC implants are biocompatible and support rapid osseointegration in the rabbit tibia and suggest that, after additional studies, they may be suitable for use as dental implants in humans.
Drummond
J F
JF
Department of Oral Health Science, University of Kentucky College of Dentistry, Lexington 40536-0084, USA.
Dominici
J T
JT
Sammon
P J
PJ
Okazaki
K
K
Geissler
R
R
Lifland
M I
MI
Anderson
S A
SA
Renshaw
W
W
D000497:Alloys; D000535:Aluminum; D000704:Analysis of Variance; D000818:Animals; D003722:Dental Alloys / Q000737:chemistry; D015921:Dental Implants; D017267:Dental Prosthesis Design; D008669:Metallurgy / Q000379:methods; D008855:Microscopy, Electron, Scanning; D009716:Numerical Analysis, Computer-Assisted; D016348:Osseointegration; D010012:Osteogenesis; D016062:Porosity; D019736:Prostheses and Implants; D011817:Rabbits; D013499:Surface Properties; D013977:Tibia; D013997:Time Factors; D014025:Titanium / Q000737:chemistry; D014639:Vanadium
D000497:Alloys; D003722:Dental Alloys; D015921:Dental Implants; D014639:Vanadium; C031462:titanium alloy (TiAl6V4); D000535:Aluminum; D014025:Titanium