[Synergistic effects of tumor necrosis factor interferon-gamma and chemotherapeutic drugs on human ovarian cancer cell lines in vitro].
Three ovarian cancer cell lines, NIH: OVCAR-3, 3AO, and AO were used in this study to assess the synergistic effect of TNF-alpha, IFN-gamma and chemotherapeutic drugs (epirubicin, carboplatin, fluorouracil). It was found that NIH: OVCAR-3 and 3AO cells were resistant to the individual treatment with TNF-alpha and IFN-gamma, while AO cells were sensitive to TNF-alpha but not to INF-gamma. Epirubicin and TNF-alpha were the two major cytotoxic agents to NIH: OVCAR-3. 3AO cells were susceptible to epirubicin, 5-Fu, TNF-alpha and IFN-gamma, while AO cells were sensitive to TNF and epirubicin. Carboplatin did not show any synergistic effect on all these three cell lines when combined with cytokines.
Li
J
J
Changhai Hospital, Second Military Medical University, Shanghai.
Hui
N
N
Zhu
L
L
D000971:Antineoplastic Combined Chemotherapy Protocols / Q000494:pharmacology*; D016190:Carboplatin / Q000008:administration & dosage; D002455:Cell Division / Q000187:drug effects; D004357:Drug Synergism; D015251:Epirubicin / Q000008:administration & dosage; D005260:Female; D005472:Fluorouracil / Q000008:administration & dosage; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology*; D010051:Ovarian Neoplasms / Q000473:pathology*; D014407:Tumor Cells, Cultured / Q000187:drug effects; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
D014409:Tumor Necrosis Factor-alpha; D015251:Epirubicin; D007371:Interferon-gamma; D016190:Carboplatin; D005472:Fluorouracil