title: Rapid internalization and degradation of surface-bound antibodies by Tritrichomonas foetus.
abstract: Tritrichomonas foetus is a protozoan parasite of cattle that can be cultured axenically. Three monoclonal antibodies specific for surface antigens of T. foetus were found to be rapidly internalized and degraded by these cells after binding. Degradation was not due to secreted or artificially liberated proteases but depended on targeting to internal degradative compartments. Radiolabeled catabolites of the antibodies were subsequently incorporated into the parasite's own proteins. Antibody degradation could be inhibited by certain protease inhibitors or lowered temperatures; a sharp reduction in degradation between 20 C and 15 C was similar to a well documented block in endocytic transport to degradative compartments of mammalian cells. Growth and proliferation of T.foetus in the continuous presence of the antibodies appeared unhindered, but there was a general shift toward expression of both more and less of each epitope among cells within each population. Subclones of these populations always exhibited striking variability in epitope expression levels, with patterns similar to the parent cultures. These findings may lead to a better understanding of how T. foetus resists host immune responses.
authors.affiliation: Veterinary Molecular Biology Laboratory, Montana State University, Bozeman 59717, USA.
authors.forename: B L
authors.initials: BL
authors.lastname: Granger
authors.forename: S J
authors.initials: SJ
authors.lastname: Warwood
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism*; D000913:Antibodies, Protozoan / Q000378:metabolism*; D000953:Antigens, Protozoan / Q000096:biosynthesis / Q000276:immunology; D000954:Antigens, Surface / Q000096:biosynthesis / Q000276:immunology; D001345:Autoradiography; D002417:Cattle; D003902:Detergents / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D010450:Endopeptidases / Q000378:metabolism; D000939:Epitopes / Q000096:biosynthesis; D005455:Fluorescent Antibody Technique; D005960:Glucosides / Q000378:metabolism; D006358:Hot Temperature; D006825:Hybridomas; D006863:Hydrogen-Ion Concentration; D051379:Mice; D017830:Octoxynol / Q000378:metabolism; D011480:Protease Inhibitors / Q000494:pharmacology; D012503:Saponins / Q000378:metabolism; D016835:Tritrichomonas foetus / Q000201:enzymology / Q000276:immunology / Q000378:metabolism*
chemical_list: D000911:Antibodies, Monoclonal; D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D000954:Antigens, Surface; D003902:Detergents; D000939:Epitopes; D005960:Glucosides; D011480:Protease Inhibitors; D012503:Saponins; C018619:octyl-beta-D-glucoside; D017830:Octoxynol; D010450:Endopeptidases