title: Gene organization in the ada-rplL region of Streptomyces virginiae.
abstract: The gene organization of a 7.4-kb region of the Streptomyces virginiae (Sv) chromosome was determined. The predicted open reading frames (ORFs) and their predicted products, in sequence order, were (i) ada, encoding adenosine deaminase [EC 3.5.4.4], (ii) aat, encoding a protein homologous to aspartate aminotransferase [EC 2.6.1.1], (iii) secE, encoding a protein involved in protein secretion, (iv) vbrA, encoding a NusG-like protein involved in antitermination of transcription as described by Okamoto et al. [J. Biol. Chem. 267 (1992) 1093-1098], and (v) rplKAJL, encoding the large subunits of the ribosomal proteins L11, L1, L10 and L12. Six of the ORFs (secE-rplL) were oriented in the same direction, but the other two (ada and aat) had the opposite orientation. The gene organization of the secE-rplL region in Sv was identical to that in Escherichia coli.
authors.affiliation: Department of Biotechnology, Faculty of Engineering, Osaka University, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Katayama
authors.forename: Y
authors.initials: Y
authors.lastname: Sakai
authors.forename: S
authors.initials: S
authors.lastname: Okamoto
authors.forename: F
authors.initials: F
authors.lastname: Ihara
authors.forename: T
authors.initials: T
authors.lastname: Nihira
authors.forename: Y
authors.initials: Y
authors.lastname: Yamada
mesh_terms: D000243:Adenosine Deaminase / Q000235:genetics*; D001219:Aspartate Aminotransferases / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics; D003001:Cloning, Molecular; D029968:Escherichia coli Proteins; D005798:Genes, Bacterial / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D015183:Restriction Mapping; D012269:Ribosomal Proteins / Q000235:genetics; D000069816:SEC Translocation Channels; D017386:Sequence Homology, Amino Acid; D013302:Streptomyces / Q000235:genetics*; D014157:Transcription Factors
chemical_list: D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D012269:Ribosomal Proteins; D000069816:SEC Translocation Channels; C065493:SecE protein, E coli; D014157:Transcription Factors; C082569:VbrA protein, Streptomyces virginiae; D001219:Aspartate Aminotransferases; D000243:Adenosine Deaminase