Modification of mitomycin C-induced clastogenicity by Terminalia catappa L. in vitro and in vivo.
The water extract of the leaves of Terminalia catappa L. was tested for inhibition of mitomycin C-induced micronuclei in CHO-K1 cells. The simultaneous and pre-treatment of CHO-K1 cells with T. catappa extract (75 and 150 micrograms/ml) significantly (P < 0.05) suppressed mitomycin C-induced micronuclei. Furthermore, gastric intubation of T. catappa extract (4.8 and 24 mg/animal per day) to male ICR mice for 8 days significantly (P < 0.01) reduced mitomycin C-induced micronuclei in peripheral blood. In addition, T. catappa dose dependently inhibited lipid peroxidation in vitro and TPA-induced hydrogen peroxide formation in human mononuclear leukocytes. The anticlastogenic effects of T. catappa in vitro and in vivo may be attributed to its antioxidative potential.
Liu
T Y
TY
Department of Medical Research, Veterans General Hospital-Taipei, Taiwan, ROC.
Ho
L K
LK
Tsai
Y C
YC
Chiang
S H
SH
Chao
T W
TW
Li
J H
JH
Chi
C W
CW
D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry*; D001628:Beverages / Q000032:analysis*; D016466:CHO Cells; D006224:Cricetinae; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D008054:Lipid Peroxides / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D015162:Micronucleus Tests; D016685:Mitomycin / Q000008:administration & dosage*; D051381:Rats
D000970:Antineoplastic Agents; D008054:Lipid Peroxides; D016685:Mitomycin; D006861:Hydrogen Peroxide