title: Biochemical characterization and protein kinase C dependency of monokine-inducing activities of Toxoplasma gondii.
abstract: Previous reports have indicated that the early induction of interleukin-12 (IL-12), tumor necrosis factor alpha (TNF-alpha), IL-1beta, and IL-10 is crucial for the establishment and regulation of host cell-mediated immunity to the intracellular protozoan parasite Toxoplasma gondii. In this study, we demonstrate that a soluble tachyzoite extract (soluble tachyzoite antigen) can trigger the expression of these four monokines by murine inflammatory macrophages. Further characterization revealed that the parasite molecules in soluble tachyzoite antigen responsible for monokine induction are heat stable at 100 degree C but differ in sensitivity to protease digestion. Thus, the tachyzoite factors that stimulate TNF-alpha and IL-to expression were found to be more resistant to treatment with proteinase K than those responsible for IL-12 and IL-10 induction. Similarly, while the factors responsible for the induction of all four monokines were found to be sensitive to periodate oxidation, the TNF-alpha-stimulating activity was partially resistant to treatment with the compound at a low concentration (1 mM). A further dichotomy in monokine induction signals was inferred from experiments with isoquinoline sulfonamide protein kinase inhibitors. The latter work suggested that the pathways for TNF-alpha and IL-1beta are protein kinase C dependent, while expression of IL-12 and expression of IL-10 share distinct signal transduction mechanisms involving other kinases. Together, these data argue that monokine induction by T. gondii is mediated by glycoproteins that may belong to distinct groups in terms of their biochemical properties and intracellular signaling pathways.
authors.affiliation: Immunobiology Section, Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892, USA.
authors.forename: E
authors.initials: E
authors.lastname: Grunvald
authors.forename: M
authors.initials: M
authors.lastname: Chiaramonte
authors.forename: S
authors.initials: S
authors.lastname: Hieny
authors.forename: M
authors.initials: M
authors.lastname: Wysocka
authors.forename: G
authors.initials: G
authors.lastname: Trinchieri
authors.forename: S N
authors.initials: SN
authors.lastname: Vogel
authors.forename: R T
authors.initials: RT
authors.lastname: Gazzinelli
authors.forename: A
authors.initials: A
authors.lastname: Sher
mesh_terms: D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D000818:Animals; D000953:Antigens, Protozoan / Q000276:immunology; D010450:Endopeptidases / Q000494:pharmacology; D005260:Female; D015870:Gene Expression; D007375:Interleukin-1 / Q000096:biosynthesis; D007546:Isoquinolines / Q000494:pharmacology; D051379:Mice; D008809:Mice, Inbred C3H; D015846:Monokines / Q000096:biosynthesis* / Q000235:genetics; D010084:Oxidation-Reduction; D010879:Piperazines / Q000494:pharmacology; D016133:Polymerase Chain Reaction; D011493:Protein Kinase C / Q000502:physiology*; D013449:Sulfonamides; D014122:Toxoplasma / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
chemical_list: D000953:Antigens, Protozoan; D007375:Interleukin-1; D007546:Isoquinolines; D015846:Monokines; D010879:Piperazines; D013449:Sulfonamides; D014409:Tumor Necrosis Factor-alpha; C094052:soluble tachyzoite antigen, Toxoplasma gondii; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; C043204:N-(2-guanidinoethyl)-5-isoquinolinesulfonamide; D011493:Protein Kinase C; D010450:Endopeptidases