Determination of gentiopicroside, mangiferin, palmatine, berberine, baicalin, wogonin and glycyrrhizin in the traditional Chinese medicinal preparation sann-joong-kuey-jian-tang by high-performance liquid chromatography.
High-performance liquid chromatography was employed to determine the contents of several marker substances such as gentiopicroside, mangiferin, palmatine, berberine, baicalin, wogonin and glycyrrhizin in Sann-Joong-Kuey-Jian-Tang. The separation was performed on a Cosmosil 5C18-AR column by gradient elution with 0.03% (v/v) phosphoric acid-acetonitrile (0 min, 90:10; 10 min, 87:13; 17-27 min, 77:23; 40 min, 62:38; 50 min, 55:45) as the mobile phase at a flow-rate of 1.0 ml/min, with detection at 254 nm. n-Propylparaben was used as the internal standard and seven regression equations revealed linear relationships between the peak-area ratios (marker substances/internal standard) and concentrations. The repeatability and reproducibility (relative standard deviation) of the method were in the ranges 0.02-1.78% and 1.44-4.95%, respectively.
Lin
S J
SJ
National Laboratories of Foods and Drugs, Department of Health, Nankang, Taipei, Taiwan.
Tseng
H H
HH
Wen
K C
KC
Suen
T T
TT
D001599:Berberine / Q000032:analysis; D001600:Berberine Alkaloids / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D044950:Flavanones; D005419:Flavonoids / Q000032:analysis; D005960:Glucosides / Q000032:analysis; D006034:Glycyrrhetinic Acid / Q000031:analogs & derivatives / Q000032:analysis; D019695:Glycyrrhizic Acid; D057888:Iridoid Glucosides; D039823:Iridoids; D011714:Pyrans / Q000032:analysis; D015203:Reproducibility of Results; D014966:Xanthenes / Q000032:analysis; D044004:Xanthones
D001600:Berberine Alkaloids; D004365:Drugs, Chinese Herbal; D044950:Flavanones; D005419:Flavonoids; D005960:Glucosides; D057888:Iridoid Glucosides; D039823:Iridoids; D011714:Pyrans; D014966:Xanthenes; D044004:Xanthones; C584126:sann-joong-kuey-jian-tang; D001599:Berberine; C012997:gentiopicroside; C013592:mangiferin; C038044:baicalin; D019695:Glycyrrhizic Acid; C005413:palmatine; D006034:Glycyrrhetinic Acid; C085514:wogonin