Role of intraduodenally administered enterostatin in rats: inhibition of food.
Central and peripheral administration of enterostatin have been reported to reduce fat or high-fat food intake in rats. Enterostatin is formed in the intestinal lumen by tryptic cleavage of pancreatic procolipase during intraluminal fat digestion. The present experiments were designed to test if enterostatin following intraintestinal infusion would affect food intake in a similar way as intracerebraventricularly or intravenously administered enterostatin. Female Sprague-Dawley rats were fitted with a duodenal catheter and adapted to feeding schedule for 6 hours each day. After 10 days enterostatin (5.65 and 11.3 nmol/kg/min) or saline were infused into the duodenum and food intake measured. Enterostatin significantly reduced high-fat food intake during the 6 hours of feeding, but had no inhibitory effect on low-fat food intake. Addition of tetracaine to the enterostatin infusates blocked the satiating potency of intestinal enterostatin. These results support the hypothesis of a preabsorptive site of action for enterostatin.
Mei
J
J
Department of Cell and Molecular Biology, University of Lund, Sweden.
Erlanson-Albertsson
C
C
D000779:Anesthetics, Local / Q000494:pharmacology; D000818:Animals; D003089:Colipases / Q000008:administration & dosage* / Q000494:pharmacology*; D004041:Dietary Fats / Q000008:administration & dosage; D004386:Duodenum / Q000187:drug effects*; D004435:Eating / Q000187:drug effects*; D004792:Enzyme Precursors; D005260:Female; D011498:Protein Precursors / Q000008:administration & dosage* / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013748:Tetracaine / Q000494:pharmacology
D000779:Anesthetics, Local; D003089:Colipases; D004041:Dietary Fats; D004792:Enzyme Precursors; D011498:Protein Precursors; D013748:Tetracaine; C037107:procolipase