Inhibition of histamine synthesis by glycyrrhetinic acid in mast cells cocultured with Swiss 3T3 fibroblasts.
The effect of glycyrrhetinic acid (18-O-beta-glycyrrhetinic acid, GA) on histamine metabolism was investigated in cultured mast cells (CMCs) cocultured with Swiss 3T3 fibroblasts. GA strongly inhibited histamine synthesis in the cocultured CMCs. Since 50 microM GA inhibited about 80% of histidine decarboxylase (HDC) activity, the inhibitory activity of GA for histamine synthesis was considered to be derived from the inhibition of HDC activity. The number of berberine-sulfate-positive cells also decreased in the presence of GA, which indicated that maturation of CMCs was inhibited by GA. Furthermore, we examined the effect of GA on the mRNA expression of novel protein kinase C delta (nPKC delta), a major isoform of CMCs, by northern blot analysis. The expression of nPKC delta mRNA in the presence of GA was significantly lower than in the absence of GA. These results suggest the possibility that the inhibition of histamine synthesis by GA is regulated by nPKC delta.
Lee
Y M
YM
Department of Microbiology and Immunology, Wonkwang University School of Medicine, Chonbuk, Republic of Korea.
Hirota
S
S
Jippo-Kanemoto
T
T
Kim
H R
HR
Shin
T Y
TY
Yeom
Y
Y
Lee
K K
KK
Kitamura
Y
Y
Nomura
S
S
Kim
H M
HM
D016475:3T3 Cells; D000818:Animals; D001599:Berberine / Q000494:pharmacology; D018920:Coculture Techniques; D004789:Enzyme Activation / Q000187:drug effects; D006034:Glycyrrhetinic Acid / Q000494:pharmacology*; D006632:Histamine / Q000096:biosynthesis*; D006633:Histamine Antagonists / Q000494:pharmacology*; D006640:Histidine Decarboxylase / Q000187:drug effects; D008407:Mast Cells / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism*; D051379:Mice; D008817:Mice, Mutant Strains; D011493:Protein Kinase C / Q000096:biosynthesis / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000187:drug effects; D019089:Stem Cell Factor / Q000187:drug effects / Q000235:genetics; D014158:Transcription, Genetic / Q000187:drug effects
D006633:Histamine Antagonists; D012333:RNA, Messenger; D019089:Stem Cell Factor; D001599:Berberine; D006632:Histamine; D011493:Protein Kinase C; D006640:Histidine Decarboxylase; D006034:Glycyrrhetinic Acid