title: Bicarbonate absorption in eel intestine: evidence for the presence of membrane-bound carbonic anhydrase on the brush border membranes of the enterocyte.
abstract: Bicarbonate absorptive fluxes through the isolated intestine of the European eel (Anguilla anguilla) were evaluated by the pH-stat method under short-circuited conditions. It was found that bicarbonate absorptive flux was dependent on the luminal Na+ and was inhibited by luminal 4-acetamido-4' stilbene-2-2' disulfonic acid (SITS; 2.5 x 10(-4) M) and luminal acetazolamide (10(-4) M), while luminal amiloride (1 mM) was without effect. Furthermore, by using brush border membrane vesicles (BBMV) isolated from eel intestine, the existence of two carbonic anhydrase (CA) isoforms, one tightly associated to the brush border membrane (BBM) and the other soluble in the cytosol, was demonstrated. The membrane-bound CA differs from the cytoplasmic isoform in that 1) it is relatively resistant to treatment with 0.045% lauryl sulfate sodium salt (SDS); 2) it is less inhibitable by ethoxzolamide and sulfanilamide; and 3) its Kmapp is significantly lower than that of the cytoplasmic isoform. These results suggest that a BBM-bound CA isozyme would play an important role in bicarbonate absorption from the lumen, facilitating the HCO3- transfer through the luminal membrane of the eel enterocyte most likely via a Na+ (HCO3-) or (OH-) cotransport system.
authors.affiliation: Dipartimento di Biologia, Università di Lecce, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Maffia
authors.forename: F
authors.initials: F
authors.lastname: Trischitta
authors.forename: M G
authors.initials: MG
authors.lastname: Lionetto
authors.forename: C
authors.initials: C
authors.lastname: Storelli
authors.forename: T
authors.initials: T
authors.lastname: Schettino
mesh_terms: D000811:Anguilla / Q000378:metabolism*; D000818:Animals; D001639:Bicarbonates / Q000493:pharmacokinetics*; D002257:Carbonic Anhydrase Inhibitors / Q000494:pharmacology; D002256:Carbonic Anhydrases / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D066298:In Vitro Techniques; D007408:Intestinal Absorption / Q000502:physiology; D007413:Intestinal Mucosa / Q000166:cytology / Q000378:metabolism*; D007527:Isoenzymes / Q000032:analysis / Q000037:antagonists & inhibitors; D008871:Microvilli / Q000201:enzymology* / Q000378:metabolism; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D000077145:Sulfanilamide; D013424:Sulfanilamides / Q000494:pharmacology
chemical_list: D001639:Bicarbonates; D002257:Carbonic Anhydrase Inhibitors; D007527:Isoenzymes; D013424:Sulfanilamides; D000077145:Sulfanilamide; D012967:Sodium Dodecyl Sulfate; D002256:Carbonic Anhydrases