title: Intensity of halothane- and hypercapnia-induced cerebral hyperemia is strain-dependent in rats.
abstract: Cerebrovascular responses to physiologic and pharmacologic stimuli vary between laboratories using different strains of the same species. We tested whether the cerebral blood flow (CBF) response to 1% halothane or hypercapnia is strain-dependent in rats. Age-matched adult male (n = 14 of each strain) Wistar, Wistar-Kyoto (WKY), and spontaneously hypertensive rats (SHR) were anesthetized with pentobarbital and mechanically ventilated. Under baseline conditions blood flow to cerebrum (microspheres) in WKY (66 +/- 5 mL.min-1.100 g-1) was less than (P < 0.05) in Wistar (88 +/- 5 mL.min-1.100g-1) and SHR (83 +/- 5 mL.min-1.100 g-1). Blood flow to brainstem was greater (P < 0.05) in Wistar (106 +/- 8 mL. min-1.100 g-1) than in WKY (71 +/- 5 mL.min-1. 100 g-1) and SHR (84 +/- 4 mL.min-1.100 g-1). In the halothane protocol (n = 8 each strain), administration of 1% halothane, during normocapnia, increased blood flow to the cerebrum in WKY (64 +/- 6 to 120 +/- 12 mL.min-1.100 g-1, P < 0.05) and SHR (78 +/- 6 to 115 +/- 8 mL.min-1.100 g-1, P < 0.05) but not Wistar rats (88 +/- 8 to 102 +/- 5 mL.min-1.100 g-1, not significant). Discontinuing halothane caused blood flow to return to baseline values. In the hypercapnia protocol (n = 6 each strain), exposure to 3% CO2 (to achieve a PaCO2 of 50-55 mm Hg) and 6% CO2 (to achieve a PaCO2 of 60-70 mm Hg) caused blood flow to the cerebrum to increase in Wistar (87 +/- 11 to 112 +/- 15 to 162 +/- 23) to similar amount as observed in WKY (69 +/- 7 to 115 +/- 13 to 162 +/- 23 mL.min-1. 100 g-1) but less than that observed in SHR (89 +/- 7 to 174 +/- 24 to 237 +/- 28 mL.min-1.100 g-1). These data demonstrate that the cerebral hyperemic response to vasodilator stimuli is strain-dependent in rats.
authors.affiliation: Department of Anesthesiology and Critical Care Medicine, Johns Hopkins Medical Institutions, Baltimore, Maryland, USA.
authors.forename: H
authors.initials: H
authors.lastname: Takahashi
authors.forename: J R
authors.initials: JR
authors.lastname: Kirsch
authors.forename: T
authors.initials: T
authors.lastname: Okada
authors.forename: R J
authors.initials: RJ
authors.lastname: Traystman
mesh_terms: D018685:Anesthetics, Inhalation / Q000494:pharmacology*; D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D001921:Brain / Q000098:blood supply* / Q000187:drug effects; D001933:Brain Stem / Q000098:blood supply / Q000187:drug effects; D002245:Carbon Dioxide / Q000008:administration & dosage / Q000097:blood; D016022:Case-Control Studies; D002540:Cerebral Cortex / Q000098:blood supply / Q000187:drug effects; D002560:Cerebrovascular Circulation / Q000187:drug effects; D006221:Halothane / Q000494:pharmacology*; D006935:Hypercapnia / Q000150:complications*; D006940:Hyperemia / Q000209:etiology* / Q000503:physiopathology; D008297:Male; D010100:Oxygen / Q000097:blood; D051381:Rats; D011918:Rats, Inbred SHR; D011919:Rats, Inbred Strains / Q000502:physiology*; D011921:Rats, Inbred WKY; D017208:Rats, Wistar; D014664:Vasodilation
chemical_list: D018685:Anesthetics, Inhalation; D002245:Carbon Dioxide; D010100:Oxygen; D006221:Halothane