title: Evaluation of non-isotopic in situ hybridization for mRNA in reactive and neoplastic lymphoid cells.
abstract: An evaluation of nonisotopic in situ hybridization (NISH) for mRNA in archival lymphoid tissue was carried out and an analysis of factors affecting the final outcome was performed. A modification of the in situ reverse transcription method for RNA preservation assessment has been used and described. We have shown that, for frozen samples mRNA detection is optimal within 3 months of the biopsy being taken, while preservation declines after 1 year of storage.
authors.affiliation: Department of Histopathology, King's College School of Medicine and Dentistry, London, England.
authors.forename: R
authors.initials: R
authors.lastname: Pitera
authors.forename: J E
authors.initials: JE
authors.lastname: Pitera
authors.forename: J R
authors.initials: JR
authors.lastname: Salisbury
mesh_terms: D001706:Biopsy; D005069:Evaluation Studies as Topic; D005629:Frozen Sections; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods*; D008198:Lymph Nodes / Q000737:chemistry; D008223:Lymphoma / Q000235:genetics*; D011309:Preservation, Biological; D012333:RNA, Messenger / Q000032:analysis*; D013997:Time Factors; D014158:Transcription, Genetic
chemical_list: D012333:RNA, Messenger