Detection of fluorescence in situ hybridization on human metaphase chromosomes by near-field scanning optical microscopy.
Fluorescence in situ hybridization signals on human metaphase chromosomes are detected by a near-field scanning optical microscope. This makes it possible to localize and identify several fluorescently labeled genomic DNA fragments on a single chromosome with a resolution superior to traditional fluorescence microscopy. Several nucleic acid probes have been used. The hybridization signals are well resolved in the near-field fluorescence images, and the exact location of the probes can be correlated to the topography as it is afforded by the shear-force feedback.
Moers
M H
MH
Department of Applied Physics, University of Twente, Enschede, The Netherlands.
Ruiter
A G
AG
Jalocha
A
A
van Hulst
N F
NF
D002878:Chromosomes, Human, Pair 1 / Q000648:ultrastructure*; D015342:DNA Probes; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008297:Male; D018625:Microscopy, Atomic Force / Q000379:methods*
D015342:DNA Probes