Calmodulin isoforms differentially enhance the binding of cauliflower nuclear proteins and recombinant TGA3 to a region derived from the Arabidopsis Cam-3 promoter.
Many stimuli increase cytoplasmic Ca2+ concentrations as an early signal transduction event and alter the patterns of nuclear gene transcription, but the mechanisms by which Ca2+ signals are transduced to the nucleus are not known. This article shows that at least four DNA binding proteins from cauliflower nuclear extracts are also calmodulin (CaM) binding proteins. CaM enhances the binding of these proteins to a C/G-box sequence element in the Arabidopsis Cam-3 promoter. Binding to the C/G-box is enhanced preferentially by the CaM isoform encoded by Cam-3. However, it is not clear whether the effect is mediated directly by CaM or indirectly through the activity of a CaM-regulated protein phosphatase. CaM also binds recombinant TGA3 and enhances its binding to the same Cam-3 promoter element. These results are consistent with the idea that a Ca(2+)-mediated signalling pathway eliciting some changes in gene expression may consist of CaM, or a structurally related Ca2+ binding protein, and transcription factors.
Szymanski
D B
DB
Department of Plant Biology, University of Illinois, Urbana 61801-3838, USA.
Liao
B
B
Zielinski
R E
RE
D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins; D001483:Base Sequence; D050976:Basic-Leucine Zipper Transcription Factors; D001937:Brassica / Q000378:metabolism*; D002147:Calmodulin / Q000096:biosynthesis / Q000378:metabolism*; D002148:Calmodulin-Binding Proteins / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism*; D018983:DNA Footprinting; D018744:DNA, Plant / Q000737:chemistry / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism*; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000378:metabolism*; D010749:Phosphoprotein Phosphatases / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000096:biosynthesis; D014157:Transcription Factors / Q000378:metabolism*
D029681:Arabidopsis Proteins; D050976:Basic-Leucine Zipper Transcription Factors; D002147:Calmodulin; D002148:Calmodulin-Binding Proteins; D018744:DNA, Plant; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D010940:Plant Proteins; D011994:Recombinant Proteins; C087770:TGA3 protein, Arabidopsis; D014157:Transcription Factors; D010749:Phosphoprotein Phosphatases