title: Sterol 14-demethylase P450 activity expressed in rat gonads: contribution to the formation of mammalian meiosis-activating sterol.
abstract: Sterol 14-demethylase P450 (P45014DM)-dependent formation of 4,4-dimethylcholesta-8,14,24-trienol, that has been reported for a mammalian meiosis-activating sterol, in rat ovaries was confirmed. Transient elevation of ovarian P45014DM activity, that might be related to the female sexual cycle of rat, was observed. The P45014DM activity of immature ovaries was induced by pregnant mare's serum gonadotropin. These facts suggest the contribution of ovarian P45014DM to the gonadotropin-dependent formation of the meiosis-activating sterol, and provide a clue to understanding the mechanism how gonadotropins initiate the resumption of meiosis of mammalian oocytes. It was also found that rat testicular P45014DM participated in the production of 4,4-dimethylcholesta-8,24-dienol that was reported for another meiosis-activated sterol isolated from bull testes.
authors.affiliation: School of Pharmaceutical Sciences, Mukogawa Women's University, Nishinomiya, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Yoshida
authors.forename: C
authors.initials: C
authors.lastname: Yamashita
authors.forename: M
authors.initials: M
authors.lastname: Noshiro
authors.forename: M
authors.initials: M
authors.lastname: Fukuda
authors.forename: Y
authors.initials: Y
authors.lastname: Aoyama
mesh_terms: D000818:Animals; D002417:Cattle; D002782:Cholestenes / Q000302:isolation & purification / Q000378:metabolism*; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis*; D005260:Female; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D006064:Gonadotropins, Equine / Q000494:pharmacology; D007700:Kinetics; D007810:Lanosterol / Q000378:metabolism; D008297:Male; D008322:Mammals; D008540:Meiosis; D009929:Organ Size / Q000187:drug effects; D010053:Ovary / Q000166:cytology / Q000187:drug effects / Q000201:enzymology*; D010088:Oxidoreductases / Q000096:biosynthesis*; D051381:Rats; D012741:Sexual Maturation; D058886:Sterol 14-Demethylase; D013737:Testis / Q000166:cytology / Q000201:enzymology*
chemical_list: D002782:Cholestenes; C548417:Cyp51 protein, rat; D006064:Gonadotropins, Equine; D007810:Lanosterol; C100324:4,4-dimethylcholesta-8,14,24-trienol; D003577:Cytochrome P-450 Enzyme System; D010088:Oxidoreductases; D058886:Sterol 14-Demethylase