Is actin polymerization relevant to neurosecretion? A study on neuroblastoma cells.
We have investigated the relevance of actin rearrangement to neurotransmitter release, in neuroblastoma cell line SH-SY5Y stimulated with carbachol (1 mM). Carbachol reversibly polymerizes actin and releases norepinephrine in undifferentiated SH-SY5Y cells as well as in tetradecanoylphorbol 13-acetate (16 nM) and retinoic acid (5 microM) differentiated SH-SY5Y cells. Microscopic analysis of F-actin distribution indicates polymerization of cortical actin. Prior treatment with iota toxin E from Clostridium perfringens inhibits both carbachol-induced actin polymerization and norepinephrine release, slightly affecting the basal actin network. These data suggest that actin polymerization is associated with norepinephrine release.
Viviani
B
B
Laboratory of Toxicology, University of Milan, Italy.
Galli
C L
CL
Marinovich
M
M
D000199:Actins / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D002217:Carbachol / Q000494:pharmacology*; D002454:Cell Differentiation; D002460:Cell Line; D006801:Humans; D007700:Kinetics; D009447:Neuroblastoma; D009638:Norepinephrine / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013997:Time Factors; D014212:Tretinoin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D000199:Actins; D014212:Tretinoin; D002217:Carbachol; D013755:Tetradecanoylphorbol Acetate; D009638:Norepinephrine