Recombinant barnase as a label in ELISA.
Recombinant barnase was proposed as a label in the enzyme-linked immunosorbent assay (ELISA). Barnase-conjugated pig transferrin was prepared by the periodate oxidation procedure. Solid-phase bound barnase activity was determined from the change in RNA-ethidium bromide complex fluorescence upon RNA hydrolysis. The sensitivity of transferrin-barnase conjugate determination in ELISA was no less than 5 ng per well. The conjugate was applied in competition ELISA for free transferrin determination.
Yazynin
S A
SA
Engelhardt Institute of Molecular Biology, The Russian Academy of Sciences, Moscow, Russian Federation.
Deyev
S M
SM
D000818:Animals; D001426:Bacterial Proteins; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D004996:Ethidium; D007202:Indicators and Reagents; D012313:RNA / Q000032:analysis; D011994:Recombinant Proteins; D012260:Ribonucleases; D013552:Swine; D014168:Transferrin
D001426:Bacterial Proteins; D007202:Indicators and Reagents; D011994:Recombinant Proteins; D014168:Transferrin; D012313:RNA; D012260:Ribonucleases; C019760:Bacillus amyloliquefaciens ribonuclease; D004996:Ethidium