title: Retroviral integrases and their cousins.
abstract: The recently determined structures of the catalytic domains of HIV integrase, avian sarcoma virus integrase and the Mu transposase are strikingly similar to each other and also exhibit significant similarity to several nucleases. All these enzymes of cut polynucleotides, leaving 3'OH and 5'PO4 groups. The integrase and transposase also possess a strand-transfer activity that splices DNA. The structural similarities among members of this superfamily of polynucleotidyl transferases suggest that they share a similar mechanism of catalysis.
authors.affiliation: Laboratory of Molecular Biology,National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892-0540, USA.
authors.forename: P
authors.initials: P
authors.lastname: Rice
authors.forename: R
authors.initials: R
authors.lastname: Craigie
authors.forename: D R
authors.initials: DR
authors.lastname: Davies
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry; D010583:Bacteriophage mu / Q000201:enzymology / Q000235:genetics; D002384:Catalysis; D004247:DNA / Q000378:metabolism; D004254:DNA Nucleotidyltransferases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D004706:Endodeoxyribonucleases / Q000737:chemistry; D004926:Escherichia coli / Q000201:enzymology; D029968:Escherichia coli Proteins; D015497:HIV-1 / Q000201:enzymology / Q000235:genetics; D019426:Integrases; D008958:Models, Molecular; D011487:Protein Conformation; D012190:Retroviridae / Q000201:enzymology* / Q000235:genetics; D012191:Retroviridae Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D016914:Ribonuclease H / Q000737:chemistry; D017386:Sequence Homology, Amino Acid; D016963:Thermus thermophilus / Q000201:enzymology; D019895:Transposases
chemical_list: D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D012191:Retroviridae Proteins; C069806:ruvC protein, E coli; D004247:DNA; D004254:DNA Nucleotidyltransferases; D019426:Integrases; D019895:Transposases; D004706:Endodeoxyribonucleases; D016914:Ribonuclease H