Effects of tunicamycin on glycoprotein antigens of Aspergillus fumigatus.
Tunicamycin, which inhibits N-glycosylation of proteins, was used as a tool to determine the type of linkage which occurs in glycoprotein antigens of Aspergillus fumigatus. When A. fumigatus extracts were electrophoretically separated and blotted then probed with anti-Aspergillus patients' sera, differences in antigenic profiles were noted when tunicamycin-treated samples were compared with controls. Tunicamycin had no detectable effect on the cellular proteinases of A. fumigatus, most of which are glycosylated. Some enzymatic components were lacking when extracellular proteinases were compared with those of control samples. The major catalase component of A. fumigatus is a concanavalin A (ConA)-binding glycoprotein. In cultures grown in the presence of tunicamycin, partially-deglycosylated catalase components were obtained which could be distinguished from the native catalase by altered mobilities in polyacrylamide gels. The effect of deglycosylation on catalase antigens was monitored using an antiserum raised to a ConA-binding fraction of A. fumigatus mycelium. These antibodies bound both to the native glycoprotein and the partially deglycosylated material. These latter two were largely unaffected when incubated with an antiserum raised to a non-ConA-binding fraction of A. fumigatus which is essentially carbohydrate free. The ability to produce partially-glycosylated antigens of A. fumigatus offers a model to study the effect of basic structural modifications on both the enzymatic and antigenic activities of these molecules.
Hearn
V M
VM
Mycology Reference Laboratory, Central Public Health Laboratory, London, Great Britain.
Shimizu
M
M
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000946:Antigens, Fungal / Q000032:analysis / Q000187:drug effects*; D001232:Aspergillus fumigatus / Q000187:drug effects* / Q000201:enzymology / Q000276:immunology; D002374:Catalase / Q000187:drug effects / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D010450:Endopeptidases / Q000187:drug effects / Q000378:metabolism; D006023:Glycoproteins / Q000032:analysis / Q000187:drug effects*; D006801:Humans; D011817:Rabbits; D014415:Tunicamycin / Q000494:pharmacology*
D000900:Anti-Bacterial Agents; D000946:Antigens, Fungal; D006023:Glycoproteins; D014415:Tunicamycin; D002374:Catalase; D010450:Endopeptidases