title: The red cell chemokine receptor is distinct from the Fy6 epitope.
abstract: BACKGROUND
  Previous studies established an association between the red cell chemokine receptor and Fy6 of the Duffy glycoprotein. The relationship between Duffy system antigens and interleukin 8 binding by red cells was examined.
  
  
  METHODS
  Interleukin 8 adsorption was measured by using red cells from owl, squirrel, and rhesus monkeys, and human red cells before and after saturation with anti-Fya (anti-Fy1), anti-Fyb (anti-Fy2), or anti-Fy6. The effect of cell saturation with interleukin 8 on Duffy system antigen expression and the effect of enzyme treatment on interleukin 8 adsorption were also examined.
  
  
  RESULTS
  Fy:1,-2,6 or Fy:-1,2,6 (owl monkey, squirrel monkey, human) red cells adsorbed interleukin 8 over a wide range of concentrations (125-1000 pg/mL). Human Fy:-1,-2,-6 red cells bound minimal interleukin 8. Human red cells saturated with corresponding antibodies displayed decreased interleukin 8 binding. Saturation of human cells with interleukin 8 failed to interfere with Duffy system antigen expression. Rhesus monkey cells lacking Fy1, Fy2, and Fy6 were able to adsorb interleukin 8 in a manner similar to Fy:1,-2,6 or Fy:-1,2,6 primate cells. Ficin treatment eliminated both Duffy system antigen expression and interleukin 8 adsorption from red cells.
  
  
  CONCLUSIONS
  The Duffy glycoprotein binds interleukin 8. Although previous studies suggested that interleukin 8 bound either directly to or close to the Fy6 epitope, the ability of Fy:-1,-2,-6 rhesus monkey cells to adsorb interleukin 8 suggests that the interleukin 8 receptor is distinct from Fy6, Fy1, and Fy2.
authors.affiliation: Department of Pathology, Deaconess Hospital, Boston, Massachusetts, USA.
authors.forename: E
authors.initials: E
authors.lastname: Hausman
authors.forename: W
authors.initials: W
authors.lastname: Dzik
authors.forename: D
authors.initials: D
authors.lastname: Blanchard
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000032:analysis*; D004375:Duffy Blood-Group System / Q000032:analysis* / Q000276:immunology; D000939:Epitopes; D004912:Erythrocytes / Q000737:chemistry*; D000882:Haplorhini; D006801:Humans; D016209:Interleukin-8 / Q000378:metabolism; D018123:Receptors, Interleukin / Q000032:analysis*; D023062:Receptors, Interleukin-8A
chemical_list: D015703:Antigens, CD; D004375:Duffy Blood-Group System; D000939:Epitopes; D016209:Interleukin-8; D018123:Receptors, Interleukin; D023062:Receptors, Interleukin-8A