title: Viability of Theileria parva trivalent stabilate after thawing and maintenance at 4 degrees C for up to 15 hours.
abstract: Two experiments were conducted to determine the effect of prolonged maintenance at 4 degrees C on thawed Theileria parva stabilates. Samples maintained at 4 degrees C for 1, 12, 15 and 18 h were all infective for cattle. Immunized cattle were able to resist potentially lethal challenges 29 days later. These results indicate that the handling of T. parva trivalent stabilates for immunization against East-Coast fever in the field should be much easier than previously envisaged.
authors.affiliation: Tick-borne Disease Vaccine Production Center, Lilongwe, Malawi.
authors.forename: F L
authors.initials: FL
authors.lastname: Musisi
authors.forename: J C
authors.initials: JC
authors.lastname: Quiroga
authors.forename: L M
authors.initials: LM
authors.lastname: Njuguna
authors.forename: S P
authors.initials: SP
authors.lastname: Kamwendo
authors.forename: K E
authors.initials: KE
authors.lastname: Chamambala
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D002417:Cattle; D003080:Cold Temperature; D005334:Fever / Q000209:etiology / Q000662:veterinary; D005615:Freezing; D007114:Immunization / Q000662:veterinary*; D016797:Theileria parva / Q000276:immunology* / Q000472:pathogenicity / Q000502:physiology; D013801:Theileriasis / Q000209:etiology / Q000276:immunology / Q000517:prevention & control*; D013997:Time Factors
chemical_list: D000913:Antibodies, Protozoan