[Comparative characterization of results of the immunoenzyme method of interleukin-1 determination in blast transformation reaction with indomethacin].
Comparison of the results of blast transformation test and enzyme immunoassay of interleukin-1 in the blood serum and culture fluid demonstrated that assessment of phytohemagglutinin (PHA)-effected level of proliferative activity of lymphocytes is more informative for the diagnosis of manifest forms of HIV infection. Combined addition of PHA and indomethacin to cell culture by shifting the balance between prostaglandin E and interleukin-1 helps assess the contribution of macrophages to the development of HIV infection.
Plavan
V V
VV
Shemshura
A B
AB
Siziakina
L P
LP
D002478:Cells, Cultured; D002648:Child; D002675:Child, Preschool; D015658:HIV Infections / Q000276:immunology*; D006801:Humans; D007124:Immunoenzyme Techniques; D007213:Indomethacin; D007375:Interleukin-1 / Q000032:analysis* / Q000097:blood; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000166:cytology / Q000276:immunology; D010835:Phytohemagglutinins; D011458:Prostaglandins E / Q000097:blood
D007375:Interleukin-1; D010835:Phytohemagglutinins; D011458:Prostaglandins E; D007213:Indomethacin
Savnitel'naia kharakteristika rezul'tatov opredeleniia interleĭkina-1 immunofermentnym metodom i v reaktsii blasttransformatsii s indometatsinom.