Q/R site editing in kainate receptor GluR5 and GluR6 pre-mRNAs requires distant intronic sequences.
RNA editing by adenosine deamination in brain-expressed pre-mRNAs for glutamate receptor (GluR) subunits alters gene-specified codons for functionally critical positions, such as the channel's Q/R site. We show by transcript analysis of minigenes transiently expressed in PC-12 cells that, in contrast to GluR-B pre-mRNA, where the two editing sites (Q/R and R/G) require base pairing with nearby intronic editing site complementary sequences (ECSs), editing in GluR5 and GluR6 pre-mRNAs recruits an ECS located as far as 1900 nucleotides distal to the Q/R site. The exon-intron duplex structure of the GluR5 and GluR6 pre-mRNAs appears to be a substrate of double-stranded RNA-specific adenosine deaminase. This enzyme when coexpressed in HEK 293 cells preferentially targets the adenosine of the Q/R site and of an unpaired position in the ECS which is highly edited in brain.
Herb
A
A
Laboratory for Molecular Neuroendocrinololgy, Zentrum für Molekulare Biologie, Heidelberg, Germany.
Higuchi
M
M
Sprengel
R
R
Seeburg
P H
PH
D000241:Adenosine / Q000737:chemistry; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000502:physiology; D017931:DNA Primers / Q000737:chemistry; D006860:Hydrogen Bonding; D007438:Introns; D051379:Mice; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D016716:PC12 Cells; D017393:RNA Editing; D012322:RNA Precursors; D012330:RNA, Double-Stranded / Q000235:genetics; D051381:Rats; D018092:Kainic Acid Receptors / Q000235:genetics*
D000241:Adenosine; D017931:DNA Primers; D012322:RNA Precursors; D012330:RNA, Double-Stranded; D018092:Kainic Acid Receptors