Analysis of T cell receptor V beta expression in rabbit T lymphocytes induced to proliferate by an HTLV-I-transformed leukemogenic T cell line.
Peripheral blood lymphocytes (PBL) from rabbits of the Chbb:HM strain proliferated in coculture with an X-ray-irradiated HTLV-I-transformed leukemogenic cell line of (B/J x Chbb:HM) F1 origin, whereas PBL from rabbits of the B/J and F1 strains hardly proliferated at all in co-culture with the same cell line. A proviral HTLV-I genome was detected in high-molecular-mass DNA from these proliferating cells. An analysis of T cell receptor V beta expression revealed that these lymphocytes were of restricted V beta subfamilies, suggesting that the preferential stimulation and transformation of lymphocytes occurred in this co-culture. Staphylococcal enterotoxins similarly stimulated lymphocytes and the proliferated lymphocytes were mostly of distinct V beta subfamilies depending on stimulator enterotoxins. These results suggested that the leukemogenic cell line possesses an antigen that preferentially stimulates lymphocytes of restricted V beta subfamilies.
Isono
T
T
Department of Microbiology, Shiga University of Medical Science, Japan.
Isegawa
Y
Y
Seto
A
A
D000818:Animals; D001483:Base Sequence; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral / Q000276:immunology*; D004768:Enterotoxins / Q000276:immunology; D015368:Human T-lymphotropic virus 1 / Q000235:genetics*; D007938:Leukemia / Q000276:immunology; D008213:Lymphocyte Activation; D008969:Molecular Sequence Data; D011817:Rabbits; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000032:analysis*; D018089:Superantigens / Q000032:analysis*; D013601:T-Lymphocytes / Q000276:immunology*
D004768:Enterotoxins; D016693:Receptors, Antigen, T-Cell, alpha-beta; D018089:Superantigens; C031818:enterotoxin B, staphylococcal