High resolution localization of cruzipain and Ssp4 in Trypanosoma cruzi by replica staining label fracture.
The replica staining label fracture technique was used to analyse the distribution of cruzipain and Ssp4 in Trypanosoma cruzi. Intense labeling for the two proteins was seen on the E fracture face of amastigote forms. Gold particles did not co-localize with the intramembranous particles. Labeling was abolished by previous treatment of the parasites with phospholipase C from Trypanosoma brucei, which removes glycosylphosphatidyl inositol (GPI) anchored proteins. These observations suggest that cruzipain and Ssp4 are attached to the parasite surface via a GPI anchor.
Nascimento
A E
AE
Laboratório de Imunopatologia Keizo Azami, Universidade Federal de Pernanbuco, Recife, Brasil.
de Souza
W
W
D000818:Animals; D000913:Antibodies, Protozoan; D000918:Antibody Specificity; D003208:Concanavalin A; D003546:Cysteine Endopeptidases / Q000032:analysis* / Q000276:immunology; D005614:Freeze Fracturing; D006023:Glycoproteins / Q000032:analysis / Q000276:immunology; D006046:Gold; D008565:Membrane Proteins / Q000032:analysis / Q000276:immunology; D015800:Protozoan Proteins / Q000032:analysis* / Q000276:immunology; D014349:Trypanosoma cruzi / Q000737:chemistry* / Q000254:growth & development
D000913:Antibodies, Protozoan; D006023:Glycoproteins; D008565:Membrane Proteins; D015800:Protozoan Proteins; D003208:Concanavalin A; D006046:Gold; D003546:Cysteine Endopeptidases; C063214:cruzipain