Preliminary experiments for the analysis of tetrahydrotetrols of benz[a]anthracene and benzo[a]pyrene derived from their hemoglobin adducts using a coupled-column high-performance liquid chromatographic system.
Improved technologies for the detection of polycyclic aromatic hydrocarbon adducts are required for human biomonitoring. Therefore, a coupled-column high-performance liquid chromatographic method, with system-integrated sample processing, has been developed and its applicability for determination of tetrahydrotetrols of polycyclic aromatic hydrocarbons in acid hydrolysates of human hemoglobin has been investigated. A novel column-switching technique applying "thermotransfer' is used to separate tetrahydrotetrols of benzo[a]pyrene and benz[a]anthracene more efficiently. Derivatives of polycyclic aromatic hydrocarbons from blood hydrolysates are concentrated on a pre-column and then transferred to the analytical column by applying an electrical current to heat the solvent eluting the pre-column. This method allows for rapid and quantitative transfer of the analytes from the pre-column to the analytical column, after HPLC-integrated sample processing.
Hackl
C
C
GSF-Forschungszentrum für Umwelt und Gesundheit GmbH, Institut für Okologische Chemie, Oberschleissheim, Germany.
Lintelmann
J
J
Sauerbrey
R
R
Kettrup
A
A
D001551:Benz(a)Anthracenes / Q000032:analysis*; D001580:Benzopyrenes / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006454:Hemoglobins / Q000737:chemistry*; D006801:Humans; D006868:Hydrolysis
D001551:Benz(a)Anthracenes; D001580:Benzopyrenes; D006454:Hemoglobins; C032107:8,9,10,11-tetrahydroxy-8,9,10,11-tetrahydrobenzanthracene; C032174:7,8,9,10-tetrahydroxytetrahydrobenzo(a)pyrene