Antibodies raised against guanosine bind to double-stranded DNA.
Antibodies elicited against guanosine have been reported to bind to single-stranded DNA. Using an avidin-biotin microELISA, we report that these antibodies also bind to double-stranded DNA. The binding is specific and is completely inhibited by the homologous hapten. The cross-reactivity of double-stranded DNA binding antibodies to single-stranded DNA is low. The antibodies are shown to bind to the topoisomers of plasmid DNA as assessed by a gel retardation assay.
Kala
K C
KC
Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore, India.
Antony
A
A
D000818:Animals; D000906:Antibodies / Q000276:immunology*; D003429:Cross Reactions; D004247:DNA / Q000378:metabolism*; D004277:DNA, Single-Stranded / Q000378:metabolism; D006151:Guanosine / Q000276:immunology* / Q000378:metabolism; D011817:Rabbits
D000906:Antibodies; D004277:DNA, Single-Stranded; D006151:Guanosine; D004247:DNA