title: Cell cycle phase duration in bone marrow cells from malnourished rats during suckling.
abstract: The generation time and duration of the different phases of the cell cycle were estimated in cultured bone marrow cells from malnourished and well-nourished rats during the lactation period by the percentage labeled mitosis (PLM) technique after a short pulse treatment with tritiated thymidine. The PLM were obtained in sequential analysis after every 3 h from removing labeled thymidine to 27 h of incubation. Results have shown a longer cell cycle time in cells from malnourished rats. The G1 + 1/2M phases are sensitive to experimental severe protein malnutrition (PCM), while S and G2 + 1/2 M phases did not show any differences in duration between malnourished and well-nourished rats.
authors.affiliation: Departamento de Ciencias de la Salud, Universidad Autónoma Metropolitana-Iztapalapa, México, D.F., Mexico.
authors.forename: J L
authors.initials: JL
authors.lastname: Gómez
authors.forename: C
authors.initials: C
authors.lastname: Campos
authors.forename: P
authors.initials: P
authors.lastname: Rangel
authors.forename: R
authors.initials: R
authors.lastname: Ortiz
mesh_terms: D000818:Animals; D000833:Animals, Suckling; D001835:Body Weight; D001853:Bone Marrow / Q000473:pathology / Q000503:physiopathology*; D002453:Cell Cycle / Q000502:physiology*; D002478:Cells, Cultured; D005260:Female; D007774:Lactation; D008297:Male; D008938:Mitosis; D009748:Nutrition Disorders / Q000503:physiopathology*; D051381:Rats; D017208:Rats, Wistar