A putative mechanism for the non-specific uptake of intact radiolabelled monoclonal antibodies in the testes and prostate.
Non-specific testicular accumulation of radiolabelled intact anti-CEA monoclonal antibody (MAb), (A431/26, Behringwerke AG) was observed in 11 out of 12 patients with the testes and prostate included in the examination field at radioimmunoscintigraphy (RIS). Previous studies have shown that placental alkaline phosphatase (PLAP) serves as an Fc-receptor, mediating IgG transport through the placenta. A closely related protein, the germ cell alkaline phosphatase (GCAP), is expressed in the testes. The testicular uptake of IgG is observed only when intact but not fragmented MAbs are used, indicating involvement of Fc-receptors. MDCK cells (dog kidney cell line) transfected with the plasmid pSVT7 containing the GCAP gene were shown to acquire the capacity to both express membrane bound GCAP and to bind IgG on the cell surface. This might indicate that GCAP is responsible for the non-specific accumulation of intact MAb in the testes and prostate often observed when intact murine MAbs are used for radioimmunolocalization (RIL).
Ahlström
K E
KE
Department of Diagnostic Radiology, Umea University, Umea, Sweden.
Makiya
R E
RE
Ullén
A P
AP
Damber
J
J
Hietala
S
S
Stigbrand
T I
TI
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000469:Alkaline Phosphatase / Q000235:genetics / Q000378:metabolism; D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism; D002272:Carcinoembryonic Antigen / Q000276:immunology; D002460:Cell Line; D004285:Dogs; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D018796:Immunoconjugates / Q000493:pharmacokinetics; D007128:Immunoglobulin Fragments / Q000378:metabolism; D007074:Immunoglobulin G / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D011467:Prostate / Q000000981:diagnostic imaging*; D016719:Radioimmunodetection; D011961:Receptors, Fc / Q000378:metabolism; D013737:Testis / Q000000981:diagnostic imaging* / Q000201:enzymology; D014162:Transfection
D000911:Antibodies, Monoclonal; D002272:Carcinoembryonic Antigen; D018796:Immunoconjugates; D007128:Immunoglobulin Fragments; D007074:Immunoglobulin G; D011961:Receptors, Fc; D000469:Alkaline Phosphatase