Macrophage cytotoxicity against murine meth A sarcoma involves nitric oxide-mediated apoptosis.
We have studied the cytotoxic effect of stimulated macrophages on Meth A tumor cells in vitro. When stimulated with interferon-gamma and soluble beta-1,3-D-glucan, macrophages exerted cytotoxicity towards syngeneic Meth A tumor cells. This cytotoxicity was associated with a high level of nitric oxide production. Both cell death and nitric oxide production were significantly inhibited by the addition of aminoguanidine, a specific inhibitor of inducible nitric oxide synthase (iNOS), to the culture medium. The cytotoxic effect was accompanied by internucleosomal cleavage of DNA as shown by electrophoresis and DNA fragmentation assay.
Sveinbjørnsson
B
B
Institute of Medical Biology, University of Tromsø, Norway.
Olsen
R
R
Seternes
O M
OM
Seljelid
R
R
D000818:Animals; D017209:Apoptosis; D002478:Cells, Cultured; D018920:Coculture Techniques; D005260:Female; D005936:Glucans / Q000494:pharmacology*; D006146:Guanidines / Q000494:pharmacology; D007369:Interferon Inducers / Q000494:pharmacology*; D007371:Interferon-gamma / Q000494:pharmacology*; D008262:Macrophage Activation / Q000187:drug effects; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D009569:Nitric Oxide / Q000502:physiology*; D009573:Nitrites / Q000378:metabolism; D009599:Nitroprusside / Q000494:pharmacology*; D011994:Recombinant Proteins; D012513:Sarcoma, Experimental / Q000276:immunology* / Q000473:pathology / Q000648:ultrastructure; D014407:Tumor Cells, Cultured; D047071:beta-Glucans
D005936:Glucans; D006146:Guanidines; D007369:Interferon Inducers; D009573:Nitrites; D011994:Recombinant Proteins; D047071:beta-Glucans; D009599:Nitroprusside; D009569:Nitric Oxide; D007371:Interferon-gamma; C033363:beta-1,3-glucan; C004479:pimagedine