title: Characterization of the acid resistance phenotype and rpoS alleles of shiga-like toxin-producing Escherichia coli.
abstract: Shiga-like toxin-producing Escherichia coli (SLTEC) strains are an important group of enteric pathogens. In this study we have examined the abilities of 58 SLTEC isolates to survive at pH 2.5 and found 13 of these isolates to be defective in acid resistance. Introduction of rpoS on a plasmid conferred acid resistance to the majority of the acid-sensitive isolates. The rpoS genes from two of these isolates were sequenced; both isolates contained lesions in the rpoS gene resulting in a nonfunctional RpoS. These results show that mutant rpoS alleles exist in natural populations of E. coli. Such mutations may play an important role in determining the infective dose of SLTEC and suggest that isolates may vary in infectivity.
authors.affiliation: Microscopy Branch, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840 USA.
authors.forename: S R
authors.initials: SR
authors.lastname: Waterman
authors.forename: P L
authors.initials: PL
authors.lastname: Small
mesh_terms: D000483:Alleles; D001426:Bacterial Proteins / Q000235:genetics*; D001427:Bacterial Toxins / Q000096:biosynthesis*; D001483:Base Sequence; D003092:Colitis / Q000209:etiology / Q000382:microbiology; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism* / Q000472:pathogenicity; D004927:Escherichia coli Infections / Q000209:etiology / Q000382:microbiology; D005798:Genes, Bacterial; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D009154:Mutation; D010641:Phenotype; D010957:Plasmids / Q000235:genetics; D012808:Sigma Factor / Q000235:genetics*; D014774:Virulence
chemical_list: D001426:Bacterial Proteins; D001427:Bacterial Toxins; D017931:DNA Primers; D004269:DNA, Bacterial; D012808:Sigma Factor; C069567:sigma factor KatF protein, Bacteria