title: Structural organization of the reduced folate carrier gene in Chinese hamster ovary cells.
abstract: The reduced folate carrier gene (rfc) encodes a putative protein that is involved in the intracellular accumulation of folates. In this report, we describe the organization of the rfc gene from Chinese hamster ovary cells. The hamster rfc gene contains 7 exons and 6 introns, which span 15.3 kilobases. It codes for two alternatively spliced messenger RNAs, one that contains all 7 exons and one that lacks exon 2 but contains the remaining 6 exons. The transcriptional start of the gene has been mapped to six sites approximately 200 base pairs upstream of the putative ATG initiation codon. The promoter region has no TATA box-like sequence but contains a consensus Sp1 binding site. This is the first report of the genomic structure of the reduced folate carrier gene from any species.
authors.affiliation: Department of Microbiology and Immunology, University of Western Ontario, London, Ontario N6A 5C1, Canada.
authors.forename: R C
authors.initials: RC
authors.lastname: Murray
authors.forename: F M
authors.initials: FM
authors.lastname: Williams
authors.forename: W F
authors.initials: WF
authors.lastname: Flintoff
mesh_terms: D017398:Alternative Splicing; D000818:Animals; D001483:Base Sequence; D001692:Biological Transport; D016466:CHO Cells; D002352:Carrier Proteins / Q000235:genetics*; D006224:Cricetinae; D017931:DNA Primers; D005091:Exons; D007438:Introns; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D008727:Methotrexate / Q000378:metabolism; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics; D058978:Reduced Folate Carrier Protein; D012260:Ribonucleases / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D002352:Carrier Proteins; D017931:DNA Primers; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D012333:RNA, Messenger; D058978:Reduced Folate Carrier Protein; D012260:Ribonucleases; D008727:Methotrexate