title: Immunophenotypic analysis of HL-60 cells during basophilic differentiation.
abstract: Differentiation along specific myeloid lineages may be accompanied by characteristic cell surface marker changes. We have examined leukemic HL-60 cell changes under conditions which induce basophilic differentiation. An increased surface expression of CD35, CD11b, and decreased expression of CD15 was found by flow cytometry during the 5-day induction period. Further investigation revealed two cell populations after 5 days in vitro: (i) a CD35-positive population (61% of cells present) containing a significant number of CD15-negative cells, and (ii) a CD15-positive/CD35-negative population. The CD35-positive subset appears to account for the majority of the basophilic cells induced under these conditions, as measured by histamine content and metachromatic staining. In addition, this subset contains a small number of early monocytic cells (CD14 and CD23 positive). The expression of CD11b is variably found on the CD15-positive/ CD35-negative subset of induced cells. These results suggest that CD35 and CD15 surface immunophenotyping can be used to map steps involved in myeloid development. A role for CD35 and CD15 in early basophil differentiation is proposed.
authors.affiliation: Department of Medicine, McMaster University, Hamilton, Ont., Canada.
authors.forename: D A
authors.initials: DA
authors.lastname: Wong
authors.forename: P
authors.initials: P
authors.lastname: Valent
authors.forename: P
authors.initials: P
authors.lastname: Bettelheim
authors.forename: J
authors.initials: J
authors.lastname: Switzer
authors.forename: J A
authors.initials: JA
authors.lastname: Denburg
mesh_terms: D015703:Antigens, CD / Q000032:analysis; D000954:Antigens, Surface / Q000032:analysis; D001491:Basophils / Q000737:chemistry* / Q000166:cytology / Q000276:immunology*; D002454:Cell Differentiation / Q000276:immunology; D002469:Cell Separation; D018922:HL-60 Cells; D006801:Humans; D016130:Immunophenotyping
chemical_list: D015703:Antigens, CD; D000954:Antigens, Surface