Characterization of a cDNA encoding murine coagulation factor VII.
The cDNA encoding murine coagulation factor VII (mfVII) was isolated and reconstructed from a lambda Zap cDNA library generated from murine liver mRNA. The cDNA contains 1903 nucleotides spanning 15 bases upstream of the 5'-translation initiation codon, an open reading frame of 1338 nucleotides, 550 nucleotides downstream of the first stop codon and a 3' poly(A) tail. The translation product is composed of a 41-amino acid signal/propeptide region followed by a 405-residue mature protein. The latter is highly homologous to that of human, rabbit, bovine, Rhesus monkey, and canine fVII. All protein domains of hfVII are strictly conserved in mfVII.
Idusogie
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Department of Chemistry and Biochemistry, University of Notre Dame, Indiana 46556, USA.
Rosen
E
E
Geng
J P
JP
Carmeliet
P
P
Collen
D
D
Castellino
F J
FJ
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D003001:Cloning, Molecular; D003327:Coronary Disease / Q000235:genetics / Q000503:physiopathology*; D018076:DNA, Complementary / Q000235:genetics*; D004285:Dogs; D005167:Factor VII / Q000235:genetics*; D005815:Genetic Code; D006801:Humans; D008253:Macaca mulatta; D051379:Mice; D008969:Molecular Sequence Data; D017354:Point Mutation; D011817:Rabbits; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
D018076:DNA, Complementary; D005167:Factor VII