[Effects of dynorphin A (1-8) on cell cycle and total cellular protein during neuronal aging in vitro].
Serum-free culture of mouse neuroblastoma cells was used as the experimental model for the study of neuronal aging, with flow cytometry of cell cycle, DNA and total cellular protein as the indices of neuronal aging. After addition of dynorphin A (1-8) 10(-7) mol.L-1 into the culture medium, the following general tendencies were obtained: (1) The number of S and G2 + M phase cells was increased and the number of G1 phase cells decreased. (2) The total cellular protein in aged experimental neural cells decreased. The results implies that Dyn A (1-8) Produced effects on cell cycle, DNA and total cellular protein in the direction of delaying neuronal aging. The relation between Dyn A (1-8) and neuronal aging merits further investigation.
Sun
X J
XJ
Laboratory of Neurobiology, Shanghai Second Medical University.
Cai
Y
Y
Su
M
M
Wang
J
J
Zhu
H
H
Lu
Q C
QC
Deng
J P
JP
Zhang
Y
Y
D000818:Animals; D002453:Cell Cycle / Q000187:drug effects*; D016922:Cellular Senescence / Q000187:drug effects; D016895:Culture Media, Serum-Free; D004247:DNA / Q000096:biosynthesis; D004399:Dynorphins / Q000494:pharmacology*; D051379:Mice; D009447:Neuroblastoma / Q000473:pathology; D010446:Peptide Fragments / Q000494:pharmacology*; D014176:Protein Biosynthesis; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000378:metabolism
D016895:Culture Media, Serum-Free; D010446:Peptide Fragments; D004399:Dynorphins; C026770:dynorphin (1-8); D004247:DNA