Expressed sequence tags identify human isologs of the ARF-dependent phospholipase D.
By searching into Expressed Sequence Tags databases (dbEST) using Blast X algorithm software and a plant phospholipase D as template, we have identified a cDNA from human brain (Z45777) which encodes for a protein similar to the amino acid region 743-929 of the human phospholipase D1 (PLD1), and a cDNA from human liver (R93485) which encodes for a protein similar to region 815-932 of PLD1. Sequence comparison between cloned phospholipases showed the presence of 3 conserved amino acid sequences: AFVGGIDLAYGRWD (box A), IIGSANINDRS (box B), and YIYIENQFFI (box C). Phylogenic analysis indicated that the cDNA from brain and liver encoded for human isologs of PLD1.
Ribbes
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Unité INSERM 326, Phospholipides Membranaires, Signalisation Cellulaire et Lipoproteines, Toulouse, France.
Henry
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Cariven
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C
Pontarotti
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Chap
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Record
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D020727:ADP-Ribosylation Factors; D000595:Amino Acid Sequence; D001921:Brain / Q000201:enzymology; D002352:Carrier Proteins / Q000378:metabolism; D016384:Consensus Sequence; D017124:Conserved Sequence; D016208:Databases, Factual; D019204:GTP-Binding Proteins / Q000378:metabolism*; D006801:Humans; D007527:Isoenzymes / Q000096:biosynthesis / Q000737:chemistry; D008099:Liver / Q000201:enzymology; D008969:Molecular Sequence Data; D012275:Oryza / Q000201:enzymology; D010739:Phospholipase D / Q000096:biosynthesis* / Q000737:chemistry* / Q000235:genetics; D010802:Phylogeny; D010944:Plants / Q000201:enzymology; D016364:Reading Frames; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D017386:Sequence Homology, Amino Acid; D016324:Sequence Tagged Sites; D012984:Software
D002352:Carrier Proteins; D007527:Isoenzymes; D010739:Phospholipase D; D019204:GTP-Binding Proteins; D020727:ADP-Ribosylation Factors