[Neoplasias in hematopoietic tissues].
Leukemia-specific chromosomal aberrations are the most reliable tumor markers. Although chromosome analysis is a powerful diagnostic tool, some aberrations like monosomy 7 in AML, 15; 17 translocation in APL and trisomy 12 in CLL are not adequately detected. Fluorescence in situ hybridization is a recent method to detect specific genetic alteration in both interphase and mitoic cells and overcomes the disadvantages of conventional cytogenetics, Southern blotting and RT-PCR. In this manuscript, the advantages and limitations of the methods used for detecting leukemia/lymphoma specific chromosomal markers were discussed.
Kamada
N
N
Department of Cancer Cytogenetics, Hiroshima University.
Ishimae
M
M
D002869:Chromosome Aberrations; D006801:Humans; D007938:Leukemia / Q000175:diagnosis*; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000175:diagnosis; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000175:diagnosis; D015470:Leukemia, Myeloid, Acute / Q000175:diagnosis; D015473:Leukemia, Promyelocytic, Acute / Q000175:diagnosis; D008223:Lymphoma / Q000175:diagnosis*; D016133:Polymerase Chain Reaction; D014178:Translocation, Genetic