title: An inverted repeat in the Tn21 integron determines the production of stem-loop DNA.
abstract: A 580-bp-long stem-loop DNA (sl-DNA) molecule was produced by a recombinant plasmid containing complete int and aadA genes from the transposon Tn21 cloned in the vector pUC18. A 50-bp-long inverted repeat found near the int gene stop codon could be involved in the generation of the sl-DNA molecule. cloning of the dhfrII gene between the plasmid replication origin and this IR produced an extended sl-DNA including the dhfrII gene. Tn21 recombination hot spot 2 was also able to direct the production of sl-DNA.
authors.affiliation: Departamento de Biología Molecular, Facultad de Medicina Universidad de Cantabria, Santander, Spain.
authors.forename: M V
authors.initials: MV
authors.lastname: Francia
authors.forename: J M
authors.initials: JM
authors.lastname: García Lobo
mesh_terms: D003062:Codon / Q000235:genetics; D004261:DNA Replication; D004251:DNA Transposable Elements / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics*; D004276:DNA, Satellite / Q000235:genetics*; D009690:Nucleic Acid Conformation; D011995:Recombination, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D014296:Trimethoprim Resistance / Q000235:genetics
chemical_list: D003062:Codon; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D004276:DNA, Satellite