title: A variant of MDCK cell line which restricted growth of influenza viruses mainly through suppression of viral primary transcription.
abstract: By serial subculture of MDCK cells which survived high multiplicity infections with AWBY-140, a weakly cytolytic mutant of influenza virus A/WSN (H1N1), we established a variant cell line (MDCK-L cells) that was uniquely resistant to infection with influenza A and B viruses, yielding 3 to 4 orders lower amount of progeny virus compared with MDCK cells. Competitive polymerase chain reaction revealed that the amount of primary transcript produced in MDCK-L cells infected with 10 PFU/cell of influenza virus A/Aichi was suppressed to 1/100 of that in MDCK cells similarly infected, although the amount of virus adsorbed to MDCK-L cells was 1/4 of MDCK cells. Even when MDCLK-L cells were infected with 40 PFU/cell of Aichi to overcome the lower amount of internalized virus in those cells, the results were the same. The synthesis of v-, c- and mRNAs, as well as proteins of infected A/Aichi was below detectable level in MDCK-L cells, in contrast with MDCK cells, where they were clearly demonstrable by ribonuclease protection assay or polyacrylamide gel electrophoresis.
authors.affiliation: Department of Virology, Jichi Medical School, Tochigi-Ken, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Jin
authors.forename: M
authors.initials: M
authors.lastname: Urabe
authors.forename: K
authors.initials: K
authors.lastname: Tobita
mesh_terms: D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D004285:Dogs; D009980:Influenza A virus / Q000235:genetics / Q000254:growth & development*; D007668:Kidney / Q000821:virology; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000096:biosynthesis; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000096:biosynthesis
chemical_list: D012367:RNA, Viral; D014764:Viral Proteins