An improved procedure for in situ RTPCR.
Direct in situ RTPCR has been successfully applied to paraffin-embedded human placental specimens. The expression of stem cell factor (SCF) mRNA visualized in cytotrophoblast and stromal cells is validated by the lack of any specific signal in parallel specimens where reverse transcription is omitted.
Volpi
L
L
Dept. of Biology and Genetics, University of Milan, Milan, Italy.
Doneda
L
L
Grimoldi
M G
MG
Pollina
E
E
Larizza
L
L
D001483:Base Sequence; D003856:Deoxyuracil Nucleotides; D006801:Humans; D008969:Molecular Sequence Data; D016612:Paraffin Embedding; D010920:Placenta / Q000737:chemistry; D016133:Polymerase Chain Reaction / Q000379:methods*; D012333:RNA, Messenger / Q000032:analysis*; D012194:RNA-Directed DNA Polymerase; D011868:Radioisotopes; D019089:Stem Cell Factor / Q000235:genetics*
D003856:Deoxyuracil Nucleotides; D012333:RNA, Messenger; D011868:Radioisotopes; D019089:Stem Cell Factor; D012194:RNA-Directed DNA Polymerase