Linear correlation between bacterial overexpression of recombinant peptides and cell light scatter.
Fusion of multiple copies of a test peptide leads to insoluble inclusion bodies. Their presence within bacteria increases either forward-angle light scattering or, to a lesser extent, right-angle light scattering. A linear correlation has been established between cell forward-angle scattering and the level of overexpression of atrial natriuretic peptide. The correlation is valid only for unlysed cells and is protein product specific.
Lavergne-Mazeau
F
F
Institut de Biotechnologie, Faculté des Sciences, Limoges, France.
Maftah
A
A
Cenatiempo
Y
Y
Julien
R
R
D009320:Atrial Natriuretic Factor / Q000096:biosynthesis*; D004926:Escherichia coli / Q000235:genetics / Q000502:physiology*; D008027:Light; D011994:Recombinant Proteins / Q000096:biosynthesis*; D012542:Scattering, Radiation
D011994:Recombinant Proteins; D009320:Atrial Natriuretic Factor