Mapping of the dilute-opisthotonus (dop) gene on chromosome 8 of the rat.
The rat dilute-opisthotonus (dop) autosomal recessive gene, causing ataxia and coat color dilution, was mapped on chromosome 8 by PCR-amplified microsatellite markers. To facilitate the linkage analysis, an intersubspecific cross with a Japanese wild rat strain was used. The recombination frequencies were 12.8% between Apoc3 and dop, and 32.1% between dop and Mylc1v. The following order of three genes is proposed; Apoc3-dop-Mylc1v. This mutation appears to be homologous to dilute-lethal (d1) of the mouse in terms of clinical symptoms, coat color effect and chromosomal location of the gene loci. Key words: ataxic mutant rat, dilute-opisthotonus (dop), gene mapping.
Ohno
K
K
School of Agricultural Sciences, Nagoya University, Japan.
Kanou
Y
Y
Oda
S
S
Wakasugi
N
N
Inouye
M
M
Yamamura
H
H
D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D002875:Chromosomes / Q000235:genetics*; D004247:DNA / Q000302:isolation & purification; D004587:Electrophoresis, Agar Gel; D005260:Female; D005787:Gene Frequency; D005808:Genes, Recessive / Q000235:genetics; D008040:Genetic Linkage; D008297:Male; D018895:Microsatellite Repeats; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D051381:Rats; D017208:Rats, Wistar
D004247:DNA