Factors conditioning detection of Taenia saginata antigens in faeces.
The conditions which optimize detection of T. saginata antigens in faeces were subject of examination. The most appropriate appeared diluent of PBS + 0.3% Tween 20. The highest effectiveness was achieved using capturing and detecting antibodies to surface antigens in comparison with somatic and metabolic ones. Two step centrifugation and final dilution of samples 1:10 (w:v) were recommended. The stability of freezed faecal supernatants after the first centrifugation was limited to 2 months. The sensitivity of capture ELISA established in our examinations allow to signalize 1 ng/ml antigen in high absorbance values. The antigens recognized by the test had MW bigger than 100,000.
Machnicka
B
B
Institute of Parasitology of the Polish Academy of Sciences, Warsaw, Poland.
Dziemian
E
E
Zwierz
C
C
D000818:Animals; D000947:Antigens, Helminth / Q000032:analysis*; D003551:Cysticercosis / Q000276:immunology / Q000469:parasitology*; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000469:parasitology*; D005615:Freezing; D006801:Humans; D011817:Rabbits; D012680:Sensitivity and Specificity; D013621:Taenia / Q000276:immunology / Q000302:isolation & purification*
D000947:Antigens, Helminth