Drug sensitivity testing for clinical samples from oesophageal cancer using adhesive tumour cell culture system.
A total of 83 specimens of surgically resected tumours from 78 patients with oesophageal cancer were assayed for drug sensitivity using an adhesive tumour cell culture system (LifeTrac CSA assay). Seventyone of 83 specimens had a sufficient number of cells to permit growth in culture and 57 of 71 (80%) were evaluable for drug response. Cells (3 x 10(3) ml-1 well-1) were cultured for 14 days and exposed to drugs on days 3-8. Growing cells were confirmed as cancer cells by immunohistochemical staining. IC90 values against several anti-cancer drugs were determined and population distributions of IC90 for each drug served as the basis for judging sensitivity. The 10th percentiles of IC90 (microgram ml-1) for CDDP, 5-FU, DOX, CPM, MTX, VP16, IFOS, VDS, BLM and CDDP + 5-FU were 0.3, 0.16, 0.005, 0.9, 0.006, 0.09, 0.8, 0.006, 0.04 and 0.15 + 0.09 respectively. The population distribution of IC90 against each drug showed a specific pattern that was very similar among histopathological gradings and stages of the disease. This system appeared to be a clinically applicable drug sensitivity test for human oesophageal cancer.
Terashima
M
M
Department of Surgery, Iwate Medical University, Morioka, Japan.
Hayashi
K
K
Fukushima
M
M
Ide
H
H
Iizuka
T
T
Kakegawa
T
T
Ando
N
N
Tanaka
O
O
Shinoda
M
M
Isono
K
K
Ishida
K
K
Ikeuchi
S
S
Endo
M
M
Takiyama
W
W
Yanagawa
T
T
D000970:Antineoplastic Agents / Q000494:pharmacology*; D002294:Carcinoma, Squamous Cell / Q000188:drug therapy* / Q000601:surgery; D002448:Cell Adhesion / Q000502:physiology; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor; D004938:Esophageal Neoplasms / Q000188:drug therapy* / Q000601:surgery; D005069:Evaluation Studies as Topic; D006801:Humans; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents