title: Structure of mitochondrial creatine kinase.
abstract: Creatine kinase (CK, EC 2.7.3.2), an enzyme important for energy metabolism in cells of high and fluctuating energy requirements, catalyses the reversible transfer of a phosphoryl goup from phosphocreatine to ADP. We have solved the structure of the octameric mitochondrial isoform, Mib-CK, which is located in the intermembrane compartment and along the cristae membranes. Mib-CK consumes ATP produced in the mitochondria for the production of phosphocreatine, which is then exported into the cytosol for fast regeneration of ATP by the cytosolic CK isoforms. The octamer has 422 point-group symmetry, and appears as a cube of side length 93 angstrom with a channel 20 angstrom wide extending along the four-fold axis. Positively charged amino acids at the four-fold faces of the octamer possibly interact with negatively charged mitochondrial membranes. Each monomer consists of a small alpha-helical domain and a large domain containing an eight-stranded antiparallel beta-sheet flanked by seven alpha-helices. The conserved residues of the CK family form a compact cluster that covers the active site between the domains.
authors.affiliation: Max-Planck-Institut für medizinische Forschung, Abteilung Biophysik, Heidelberg, Germany.
authors.forename: K
authors.initials: K
authors.lastname: Fritz-Wolf
authors.forename: T
authors.initials: T
authors.lastname: Schnyder
authors.forename: T
authors.initials: T
authors.lastname: Wallimann
authors.forename: W
authors.initials: W
authors.lastname: Kabsch
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002645:Chickens; D003402:Creatine Kinase / Q000737:chemistry*; D007425:Intracellular Membranes / Q000201:enzymology; D008929:Mitochondria, Heart / Q000201:enzymology; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011487:Protein Conformation
chemical_list: D003402:Creatine Kinase