Expression domains of a zebrafish homologue of the Drosophila pair-rule gene hairy correspond to primordia of alternating somites.
her1 is a zebrafish cDNA encoding a bHLH protein with all features characteristic of members of the Drosophila HAIRY-E(SPL) family. During late gastrulation stages, her1 is expressed in the epibolic margin and in two distinct transverse bands of hypoblastic cells behind the epibolic front. After completion of epiboly, this pattern persists essentially unchanged through postgastrulation stages; the marginal domain is incorporated in the tail bud and, depending on the time point, either two or three paired bands of expressing cells are present within the paraxial presomitic mesoderm separated by regions devoid of transcripts. Labelling of cells within the her1 expression domains with fluorescein-dextran shows that the cells in the epibolic margin and the tail bud are not allocated to particular somites. However, allocation of cells to somites occurs between the marginal expression domain and the first expression band, anterior to it. Moreover, the her1 bands, and the intervening non-expressing zones, each represents the primordium of a somite. This expression pattern is highly reminiscent of that of Drosophila pair-rule genes. A possible participation of her1 in functions related to somite formation is discussed.
Müller
M
M
Institut für Entwicklungsbiologie, Universität zu Köln, Germany.
v Weizsäcker
E
E
Campos-Ortega
J A
JA
D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins; D018076:DNA, Complementary / Q000032:analysis; D005775:Gastrula / Q000166:cytology; D018507:Gene Expression Regulation, Developmental / Q000235:genetics*; D018398:Homeodomain Proteins / Q000235:genetics*; D017403:In Situ Hybridization; D007301:Insect Hormones / Q000235:genetics*; D019476:Insect Proteins; D008648:Mesoderm / Q000166:cytology / Q000502:physiology; D009024:Morphogenesis / Q000235:genetics; D012097:Repressor Proteins; D014157:Transcription Factors / Q000235:genetics*; D014158:Transcription, Genetic; D015027:Zebrafish / Q000196:embryology / Q000235:genetics*
D051792:Basic Helix-Loop-Helix Proteins; D018076:DNA, Complementary; D018398:Homeodomain Proteins; D007301:Insect Hormones; D019476:Insect Proteins; D012097:Repressor Proteins; D014157:Transcription Factors; C061841:hairy protein, insect