A comparative study of detection of hepatitis E virus RNA by RT-PCR and digoxin probe techniques.
Techniques for detecting hepatitis E virus nucleic acid by RT-PCR and digoxin probe spot hybridization tests were developed. The examination by RT-PCR test of 250 stored stool specimens from patients with acute hepatitis E gave a positive rate of 40.9% and was positive in one stool specimen collected 28 days after the patient had contracted the illness. Serologic examination yielded a positive rate of 66.7%, and also utilizing the DIG probe labelled with PCR product it was 66.6%. RT-PCR with the digoxin probe proved to be suitable for clinical diagnosis and basic research on hepatitis E.
Zhao
S Y
SY
Department of Infectious Diseases, People's General Hospital of Xinjiang, P.R. China.
Zhou
L Y
LY
Zhou
W Y
WY
Yang
Z L
ZL
Zhong
Y N
YN
D001483:Base Sequence; D015342:DNA Probes; D004077:Digoxin; D016751:Hepatitis E / Q000821:virology; D016752:Hepatitis E virus / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D012367:RNA, Viral / Q000032:analysis*; D014158:Transcription, Genetic
D015342:DNA Probes; D012367:RNA, Viral; D004077:Digoxin