[The effect of the conditions for Neisseria meningitidis cultivation on the protein composition in the protein-polysaccharide complex--the base of meningococcal serogroup B vaccine].
New data concerning the influence of cultivation conditions on the synthesis aimed at producing immunologically active antigens of group B Neisseria meningitides were obtained. The combination of conditions (deficiency in ions of iron, pH of the medium, growth phase of the culture) capable of essentially increasing (9- to 28-fold) the content of iron-dependent protein with a mol. wt. of 75-76 kD, was selected. At the same time the content of other immunologically active proteins with mol. wt. of 92-94 kD, 100-102 kD and 141-144 kD perceptibly increased (1.5 to 2.2-fold). Group B meningococcal vaccine prepared on the basis of native protein-polysaccharide complex with a higher content of the above-mentioned species-specific proteins may be expected to have a wider spectrum of immunological action.
Kuvakina
V I
VI
Basnak'ian
I A
IA
Aleksakhina
N N
NN
Artiukhova
I I
II
Bobyleva
G V
GV
Omarova
S M
SM
D001425:Bacterial Outer Membrane Proteins / Q000032:analysis / Q000096:biosynthesis* / Q000276:immunology; D001426:Bacterial Proteins; D001428:Bacterial Vaccines / Q000032:analysis / Q000096:biosynthesis*; D003470:Culture Media; D006863:Hydrogen-Ion Concentration; D007501:Iron / Q000032:analysis / Q000276:immunology / Q000378:metabolism*; D033862:Iron-Binding Proteins; D008970:Molecular Weight; D009345:Neisseria meningitidis / Q000145:classification / Q000254:growth & development* / Q000276:immunology; D033902:Periplasmic Binding Proteins; D011135:Polysaccharides, Bacterial / Q000032:analysis / Q000096:biosynthesis* / Q000276:immunology; D012703:Serotyping
D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D003470:Culture Media; D033862:Iron-Binding Proteins; D033902:Periplasmic Binding Proteins; D011135:Polysaccharides, Bacterial; D007501:Iron
Vliianie usloviĭ kul'tivirovaniia Neisseria meningitidis na sostav belok v belkovo-polisakharidnom komplekse--osnove meningokokkovoĭ vaktsiny serogruppy B.