Measurement of ABH blood group substances in human saliva by immunoassay using artificial antigens as standard substances.
Introducing artificial antigens (trisaccharide-albumin complexes for A, B and H activities) as reference substances for immunoassay, blood group substances, A, B, and H in human saliva were measured. A good correlation between hemagglutination inhibition titers and concentrations of A, B, and H in saliva was demonstrated. Since the anti-H 1E3 used showed the specificity for both H type 1 and H type 2 chains and anti-H 224 for H type 2 chain, we measured H type (1 and 2) and H type 2 separately, and found 10- to 20-fold higher concentration of H type (1 and 2) compared with that of H type 2 in human saliva. The H type 2 was not found in all the saliva samples from nonsecretors irrespective of ABO subtypes. The results suggest that about 90% of A and B blood group substances in human saliva is also type 1 chain.
Wang
B
B
Department of Legal Medicine, Kurume University School of Medicine, Fukuoka, Japan.
Wang
Q
Q
Koda
Y
Y
Akiyama
K
K
Kimura
H
H
D000017:ABO Blood-Group System / Q000032:analysis*; D000911:Antibodies, Monoclonal; D006801:Humans; D007118:Immunoassay; D012015:Reference Standards; D012463:Saliva / Q000276:immunology*; D014312:Trisaccharides
D000017:ABO Blood-Group System; D000911:Antibodies, Monoclonal; D014312:Trisaccharides