Expression of the Renilla reniformis luciferase gene in mammalian cells.
A cDNA encoding the Renilla reniformis luciferase was expressed in similan and murine cells in a transient and stable manner, respectively. Light emission catalyzed by luciferase was detected from transfected cells both in vitro and in vivo. This work establishes the Renilla luciferase gene as a new efficient marker of gene expression in mammalian cells.
Lorenz
W W
WW
Department of Biochemistry, University of Georgia, Athens 30602, USA.
Cormier
M J
MJ
O'Kane
D J
DJ
Hua
D
D
Escher
A A
AA
Szalay
A A
AA
D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D002460:Cell Line; D002522:Chlorocebus aethiops; D003063:Cnidaria / Q000201:enzymology* / Q000235:genetics; D017931:DNA Primers; D018076:DNA, Complementary; D005347:Fibroblasts; D015870:Gene Expression; D008156:Luciferases / Q000096:biosynthesis* / Q000378:metabolism; D008163:Luminescent Measurements; D008322:Mammals; D051379:Mice; D018715:Microscopy, Video; D008969:Molecular Sequence Data; D010957:Plasmids; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000378:metabolism; D014162:Transfection
D017931:DNA Primers; D018076:DNA, Complementary; D011994:Recombinant Proteins; D008156:Luciferases