title: Involvement of LFA-1/ICAM-2 adhesive interactions and PKC in activation-induced cell death following SEB rechallenge.
abstract: Ligation of T-cell receptor (TCR) causes mature T cells to proliferate or, on re-exposure to antigen, can cause them to die by activation-induced cell death (AICD). In proliferative responses, costimulatory and adhesive interactions are required and activation of protein kinase C (PKC) has been shown to be essential. Whether or not interactions involving costimulatory signals and PKC have a role in facilitating AICD remains unclear. Here we have examined the role of CD28/B7 and leucocyte function associated antigen-1 (LFA-1)/intracellular adhesion molecule (ICAM) mediated interactions in AICD triggered by staphylococcal enterotoxin B (SEB) in murine lymph node T cells. We show that, after a primary proliferative response to SEB, LFA-1/ICAM-2 adhesive interactions can play a part in AICD following SEB rechallenge, while B7 and ICAM-1 mediated interactions are not essential for this process. In addition, using a highly selective PKC inhibitor, Ro31.8425, we show that PKC activation is essential for the regulation of AICD by SEB rechallenge.
authors.affiliation: Department of Anatomy/Centre for Clinical Research in Immunology and Signalling, University of Birmingham, UK.
authors.forename: E Z
authors.initials: EZ
authors.lastname: Tchilian
authors.forename: G
authors.initials: G
authors.lastname: Anderson
authors.forename: N C
authors.initials: NC
authors.lastname: Moore
authors.forename: J J
authors.initials: JJ
authors.lastname: Owen
authors.forename: E J
authors.initials: EJ
authors.lastname: Jenkinson
mesh_terms: D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology; D015703:Antigens, CD / Q000276:immunology*; D017209:Apoptosis / Q000276:immunology*; D018122:B7-1 Antigen / Q000032:analysis; D015815:Cell Adhesion Molecules / Q000276:immunology*; D018929:Cell Culture Techniques; D004768:Enterotoxins / Q000276:immunology*; D005260:Female; D006168:Guinea Pigs; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D008198:Lymph Nodes / Q000276:immunology; D008213:Lymphocyte Activation; D016169:Lymphocyte Function-Associated Antigen-1 / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D011493:Protein Kinase C / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D015703:Antigens, CD; D018122:B7-1 Antigen; D015815:Cell Adhesion Molecules; D004768:Enterotoxins; D000949:Histocompatibility Antigens Class II; C065289:ICAM2 protein, human; D016169:Lymphocyte Function-Associated Antigen-1; C031818:enterotoxin B, staphylococcal; D011493:Protein Kinase C