Structural and immunological characterization of Burkholderia pseudomallei O-polysaccharide-flagellin protein conjugates.
The O-polysaccharide moiety of Burkholderia pseudomallei 319a lipopolysaccharide was covalently linked to flagellin protein isolated from the same strain. A glycoconjugate incorporating adipic acid dihydrazide as a spacer molecule elicited high-titer immunoglobulin G responses to both the protein and carbohydrate components of the construct. This immunoglobulin G was capable of protecting diabetic rats from challenge with a heterologous B. pseudomallei strain.
Brett
P J
PJ
Department of Microbiology and Infectious Diseases, Faculty of Medicine, University of Calgary Health Sciences Centre, Alberta, Canada.
Woods
D E
DE
D000818:Animals; D000907:Antibodies, Bacterial / Q000008:administration & dosage / Q000096:biosynthesis; D016957:Burkholderia pseudomallei / Q000737:chemistry* / Q000276:immunology*; D003921:Diabetes Mellitus, Experimental / Q000150:complications / Q000276:immunology; D005408:Flagellin / Q000737:chemistry* / Q000276:immunology*; D006001:Glycoconjugates / Q000737:chemistry / Q000276:immunology; D007116:Immunization, Passive; D007120:Immunochemistry; D018796:Immunoconjugates / Q000737:chemistry* / Q000276:immunology*; D007074:Immunoglobulin G / Q000008:administration & dosage / Q000096:biosynthesis; D008554:Melioidosis / Q000150:complications / Q000276:immunology / Q000517:prevention & control; D015394:Molecular Structure; D019081:O Antigens / Q000737:chemistry* / Q000276:immunology*; D011817:Rabbits; D051381:Rats; D017207:Rats, Sprague-Dawley
D000907:Antibodies, Bacterial; D006001:Glycoconjugates; D018796:Immunoconjugates; D007074:Immunoglobulin G; D019081:O Antigens; D005408:Flagellin