title: A blinded comparison of two methods of viral susceptibility testing: plaque reduction assay versus microplate in situ ELISA.
abstract: Viral susceptibility testing has been shown to have a role in the management of patients with herpes simplex infections. In this study, 25 isolates of herpes simplex virus representing a broad spectrum of acyclovir-susceptible and -resistant phenotypes were tested using a microplate in situ enzyme-linked immunosorbent assay (MISE). This method is objective and more rapid than the traditional plaque reduction assay (PRA). The previously derived PRA results were not known at the time of testing with the MISE method. The correlation coefficient between PRA and MISE was 0.85. Agreement on sensitive or resistant was reached for 21 of 25 isolates. The standardised microplate in situ ELISA was found to be an acceptable alternative to the plaque reduction assay.
authors.affiliation: Department of Microbiology, Royal Perth Hospital, Australia.
authors.forename: B J
authors.initials: BJ
authors.lastname: Leahy
authors.forename: K J
authors.initials: KJ
authors.lastname: Christiansen
authors.forename: P
authors.initials: P
authors.lastname: Collins
mesh_terms: D000212:Acyclovir / Q000494:pharmacology*; D000998:Antiviral Agents / Q000494:pharmacology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D018139:Simplexvirus / Q000187:drug effects* / Q000302:isolation & purification; D010948:Viral Plaque Assay / Q000379:methods*
chemical_list: D000998:Antiviral Agents; D000212:Acyclovir