Retrieval of leukocyte antigens in paraffin-embedded rat tissues.
We compared the immunohistochemical reactivity of various rat leukocyte antigens in frozen and paraffin-embedded thymus, spleen, abdominal lymph node, liver, and brain tissues of healthy Sprague-Dawley rats, fixed in various fixatives. Immune reactivity after fixation in Methacarn was superior to that of 4% neutral buffered formalin, a mercury-based fixative (B-5), or Carnoy. Microwave (MW) antigen retrieval (AR) enhanced antigen reactivity. Ten of the 11 leukocyte antigens studied could be retrieved in Methacarn-fixed, paraffin-embedded sections with a reactivity comparable to that obtained on frozen sections.
Shetye
J D
JD
Department of Oncology-Pathology, Karolinska Institute and Hospital, Stockholm, Sweden.
Scheynius
A
A
Mellstedt
H T
HT
Biberfeld
P
P
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology; D001921:Brain / Q000276:immunology / Q000648:ultrastructure; D005404:Fixatives; D006649:Histocompatibility Antigens / Q000032:analysis*; D007150:Immunohistochemistry / Q000379:methods*; D007694:Killer Cells, Natural / Q000276:immunology; D007962:Leukocytes / Q000276:immunology*; D008198:Lymph Nodes / Q000276:immunology / Q000648:ultrastructure; D008264:Macrophages / Q000276:immunology; D051379:Mice; D016612:Paraffin Embedding; D051381:Rats; D017207:Rats, Sprague-Dawley; D013154:Spleen / Q000276:immunology / Q000648:ultrastructure; D013601:T-Lymphocytes / Q000276:immunology; D013950:Thymus Gland / Q000276:immunology / Q000648:ultrastructure
D000911:Antibodies, Monoclonal; D005404:Fixatives; D006649:Histocompatibility Antigens