v-src interferes with the in vitro erythropoietic stimulatory ability of spleen stromal cells through repression of vascular cell adhesion molecule-1 and stem cell factor.
A mouse spleen stromal cell line, MSS62, can create an in vitro erythropoietic microenvironment in which development of erythropoietin-responsive progentor cells is stimulated by cell-cell contact via stem cell factor (SCF)/c-Kit and vascular cell adhesion molecule-1 (VCAM-1)/very late activation antigen-4 (VLA-4) interactions between stromal and erythroid cells. To find out the effect of src on the erythropoietic microenvironment, MSS62 cells were transfected with v-src oncogene, and its effect on erythropoietic stimulatory activity was measured. Transfectants with high v-Src activity showed reduction in erythropoietic stimulatory activity. A decrease in cell-surface VCAM-1 and SCF mRNA was accompanied by high v-Src activity. These results suggest that v-Src interferes with the erythropoietic stimulatory activity of the stromal cells through repression of VCAM-1 and SCF.
Yanai
N
N
Department of Cell Biology, Institute of Development, Aging and Cancer, Tohoku University, Sendai, Japan.
Shimizu
A
A
Koguma
M
M
Obinata
M
M
D000818:Animals; D002448:Cell Adhesion; D002455:Cell Division; D002460:Cell Line; D018920:Coculture Techniques; D003114:Colony-Forming Units Assay; D017077:Culture Media, Conditioned; D004920:Erythropoiesis; D005434:Flow Cytometry; D016391:Genes, src; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D004915:Leukemia, Erythroblastic, Acute / Q000503:physiopathology; D051379:Mice; D015688:Oncogene Protein pp60(v-src) / Q000096:biosynthesis / Q000502:physiology*; D010766:Phosphorylation; D011994:Recombinant Proteins / Q000096:biosynthesis; D013154:Spleen / Q000166:cytology; D019089:Stem Cell Factor / Q000502:physiology*; D017154:Stromal Cells; D014162:Transfection; D014407:Tumor Cells, Cultured; D019010:Vascular Cell Adhesion Molecule-1 / Q000502:physiology*
D017077:Culture Media, Conditioned; D011994:Recombinant Proteins; D019089:Stem Cell Factor; D019010:Vascular Cell Adhesion Molecule-1; D015688:Oncogene Protein pp60(v-src)