Zfp60, a mouse zinc finger gene expressed transiently during in vitro muscle differentiation.
The complete cDNA coding sequence of the zinc finger gene Zfp60 is reported. The predicted amino acid sequence of the Zfp60 protein has been found to contain 19 zinc finger motives clustered at the C-terminus. At its N-terminus, Zfp60 shares with other members of the zinc finger gene family two additional conserved amino acid modules named Kruppel Associated Boxes (KRAB). The expression patterns of Zfp60, MyoD and MHC mRNAs have been followed during in vitro myogenic differentiation of C2 cells. We show that the bacterial produced Zfp60 protein binds DNA only in presence of zinc ions. Zfp60 locus has been mapped in chromosome 7, where other Zfp loci are localised.
Perez
M
M
Istituto Pasteur-Fondazione Cenci Bolognetti, Dipartimento di Biopatologia Umana, Sezione di Biologia Cellulare, Università di Roma La Sapienza, Rome, Italy.
Rompato
G
G
Corbi
N
N
De Gregorio
L
L
Dragani
T A
TA
Passananti
C
C
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002454:Cell Differentiation; D002460:Cell Line; D002874:Chromosome Mapping; D004247:DNA / Q000378:metabolism; D017931:DNA Primers; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D009132:Muscles / Q000166:cytology / Q000378:metabolism*; D011485:Protein Binding; D014407:Tumor Cells, Cultured; D016335:Zinc Fingers / Q000235:genetics*
D017931:DNA Primers; D004268:DNA-Binding Proteins; C100343:Zfp60 protein, mouse; D004247:DNA