A novel PNET cell line with melanotic differentiation.
The primitive neuroectodermal tumor (PNET) cell line, ONS-99, was established from a human melanotic neuroectodermal tumor of infancy (MNTI). ONS-99 cells were polygonal adherent cells and transplantable to BALB/c-nu/nu mouse. ONS-99 cells were positive for NSE, HMB-45 (melanoma-associated antigen) and N-myc, but lacked the antigenic features of terminally differentiated neurons or glias. ONS-99 cells had many intermediate filaments in the cytoplasm and tight intercellular junctions with scant cytoplasmic structures by electron microscopy. To date, ONS-99 is the only example of an established PNET cell line with melanotic differentiation.
Moriuchi
S
S
Department of Neurosurgery, Center for Adult Diseases, Osaka, Japan.
Shimizu
K
K
Miyao
Y
Y
Hayakawa
T
T
D000818:Animals; D002455:Cell Division; D006801:Humans; D007223:Infant; D007621:Karyotyping; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008854:Microscopy, Electron; D017600:Neuroectodermal Tumor, Melanotic / Q000235:genetics / Q000276:immunology / Q000473:pathology*; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured / Q000473:pathology*