title: Evidence that biliverdin-IX beta reductase and flavin reductase are identical.
abstract: A search of the database shows that human biliverdin-IX beta reductase and flavin reductase are identical. We have isolated flavin reductase from bovine erythrocytes and show that the activity co-elutes with biliverdin-IX beta reductase. Preparations of the enzyme that are electrophoretically homogeneous exhibit both flavin reductase and biliverdin-IX beta reductase activities; however, they are not capable of catalysing the reduction of biliverdin-IX alpha. Although there is little obvious sequence identity between biliverdin-IX alpha reductase (BVR-A) and biliverdin-IX beta reductase (BVR-B), they do show weak immunological cross-reactivity. Both enzymes bind to 2',5'-ADP-Sepharose.
authors.affiliation: Department of Biochemistry, Trinity College, Dublin, Ireland.
authors.forename: F
authors.initials: F
authors.lastname: Shalloe
authors.forename: G
authors.initials: G
authors.lastname: Elliott
authors.forename: O
authors.initials: O
authors.lastname: Ennis
authors.forename: T J
authors.initials: TJ
authors.lastname: Mantle
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001664:Biliverdine / Q000378:metabolism*; D002417:Cattle; D002850:Chromatography, Gel; D004912:Erythrocytes / Q000201:enzymology*; D038181:FMN Reductase; D006801:Humans; D008969:Molecular Sequence Data; D009247:NADH, NADPH Oxidoreductases / Q000737:chemistry*; D010088:Oxidoreductases / Q000737:chemistry*; D044925:Oxidoreductases Acting on CH-CH Group Donors; D010446:Peptide Fragments / Q000737:chemistry; D016415:Sequence Alignment; D017421:Sequence Analysis
chemical_list: D010446:Peptide Fragments; D010088:Oxidoreductases; D044925:Oxidoreductases Acting on CH-CH Group Donors; C019789:biliverdin reductase; D038181:FMN Reductase; D009247:NADH, NADPH Oxidoreductases; D001664:Biliverdine