Analysis of CSK homologous kinase (CHK/HYL) in hematopoiesis by utilizing gene knockout mice.
CHK/HYL is a non-receptor tyrosine kinase that belongs to CSK (C-terminal Src kinase) family. Northern blotting and RT-PCR analyses showed that CHK/HYL was expressed in large spectrum of hematopoietic cells except for erythroid cells and brain. To explore the function of CHK/HYL in hematopoietic cells, we generated CHK/HYL deficient mice. The mutant mice were apparently normal and fertile, while CSK knockout mice died until E11.5 from a defect in the neural tube formation. Hematological observations including blood counts and FACS analysis showed no significant abnormalities in CHK/HYL mutant mice. CHK/HYL did not affect the activity of Src, Hck, and Fgr in cultured bone marrow cells, although CSK negatively regulates Src family kinases. These results suggest that CHK/HYL might not have the same function as CSK.
Hamaguchi
I
I
Department of Cell Differentiation, Kumamoto University School of Medicine, Japan.
Yamaguchi
N
N
Suda
J
J
Iwama
A
A
Hirao
A
A
Hashiyama
M
M
Aizawa
S
S
Suda
T
T
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D001483:Base Sequence; D001772:Blood Cell Count; D001853:Bone Marrow / Q000201:enzymology; D002478:Cells, Cultured; D002678:Chimera; D003433:Crosses, Genetic; D017931:DNA Primers; D005260:Female; D015870:Gene Expression; D006410:Hematopoiesis; D006412:Hematopoietic Stem Cells / Q000201:enzymology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008969:Molecular Sequence Data; D016296:Mutagenesis; D009928:Organ Specificity; D011505:Protein-Tyrosine Kinases / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D016392:Proto-Oncogene Proteins pp60(c-src); D013154:Spleen; D013601:T-Lymphocytes / Q000276:immunology; D013950:Thymus Gland
D017931:DNA Primers; C086603:Matk protein, mouse; D011505:Protein-Tyrosine Kinases; D016392:Proto-Oncogene Proteins pp60(c-src)