An affinity method for the purification of mannose 6-phosphate receptor proteins (MPR 215) from rat tissues and goat liver.
An affinity matrix (Sepharose 6B-divinyl sulfone-pentaphosphomannan) has been developed which can be efficiently used for the purification of the MPR 215 from different tissues of rat as well as from goat liver. The matrix developed is relatively easy to prepare compared with the available procedures, and can be used for the purification of similar receptor proteins from other sources.
Kumar
N S
NS
Biochemistry Department, School of Life Sciences, University of Hyderabad, India.
D000818:Animals; D001921:Brain / Q000378:metabolism; D002846:Chromatography, Affinity / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel; D006041:Goats; D007202:Indicators and Reagents; D008099:Liver / Q000378:metabolism*; D008297:Male; D008970:Molecular Weight; D051381:Rats; D017208:Rats, Wistar; D017527:Receptor, IGF Type 2 / Q000302:isolation & purification*; D013737:Testis / Q000378:metabolism
D007202:Indicators and Reagents; D017527:Receptor, IGF Type 2