cDNA cloning of thylakoid-bound ascorbate peroxidase in pumpkin and its characterization.
A cDNA for thylakoid-bound ascorbate peroxidase of pumpkin was cloned and characterized. Thylakoid-bound ascorbate peroxidase had a high similarity to cytosolic ascorbate peroxidases, and the precursor contained a transit peptide to chloroplasts at its amino-terminus and a putative membrane-spanning region at its carboxy-terminus.
Yamaguchi
K
K
Department of Cell Biology, National Institute for Basic Biology, Okazaki, Japan.
Hayashi
M
M
Nishimura
M
M
D000595:Amino Acid Sequence; D060387:Ascorbate Peroxidases; D001483:Base Sequence; D002736:Chloroplasts / Q000201:enzymology; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics*; D005638:Fruit / Q000201:enzymology* / Q000235:genetics*; D008969:Molecular Sequence Data; D010544:Peroxidases / Q000235:genetics* / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
D017931:DNA Primers; D018076:DNA, Complementary; D018744:DNA, Plant; D010544:Peroxidases; D060387:Ascorbate Peroxidases