title: Enhanced resolution in the fingerprinting of short-chain oligonucleotides using the dodecasodium salt of phytic acid in capillary electrophoresis.
abstract: The inclusion of the dodecasodium salt of phytic acid in separation buffers has been found to improve considerably the separation of a number of synthetic short-chain oligonucleotides. A combination of the simplicity and the improved resolving power of this methodology allows the facile analysis of mixtures of such compounds. The presence of a large concentration of sodium ions reduces electroosmotic mobility due to more efficient shielding of negative charges on the inner surface of the capillary wall. This, in turn, leads to a more gradual fall in potential between this surface and the center of the capillary.
authors.affiliation: SmithKline Beecham Pharmaceuticals, The Frythe, Welwyn, Herts, UK.
authors.forename: G
authors.initials: G
authors.lastname: Okafo
authors.forename: G
authors.initials: G
authors.lastname: Harland
authors.forename: P
authors.initials: P
authors.lastname: Camilleri
mesh_terms: D001483:Base Sequence; D016172:DNA Fingerprinting / Q000379:methods*; D019075:Electrophoresis, Capillary; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000032:analysis*; D010833:Phytic Acid / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D009841:Oligonucleotides; D010833:Phytic Acid