Action of interferon alpha and beta on four human melanoma cell lines in vitro.
Four human melanoma cell lines with different copy numbers of chromosomes 9 and 21q, as studied by the G-band technique, fluorescent in situ hybridisation (FISH) and Polymerase chain reaction (PCR), were tested for their sensitivity to Interferon-alpha (IFN-alpha) and Interferon-beta (IFN-beta) in relation to dosage of interferon genes (#9) and interferon receptor genes (#21p). The two most sensitive cell lines were those containing the highest numbers of #9 per cell, while the number of #21q copies (receptor genes) seemed to have no influence on the interferon sensitivity.
Köpf
J
J
Department of Oncology, University of Göteborg, Sweden.
Hanson
C
C
Delle
U
U
Weimarck
A
A
Stierner
U
U
D002899:Chromosomes, Human, Pair 9; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D016898:Interferon-alpha / Q000235:genetics / Q000494:pharmacology*; D016899:Interferon-beta / Q000235:genetics / Q000494:pharmacology*; D008545:Melanoma / Q000188:drug therapy* / Q000235:genetics; D016133:Polymerase Chain Reaction; D014178:Translocation, Genetic; D014407:Tumor Cells, Cultured
D016898:Interferon-alpha; D016899:Interferon-beta