Evaluation of non-isotopic in situ hybridization for mRNA in reactive and neoplastic lymphoid cells.
An evaluation of nonisotopic in situ hybridization (NISH) for mRNA in archival lymphoid tissue was carried out and an analysis of factors affecting the final outcome was performed. A modification of the in situ reverse transcription method for RNA preservation assessment has been used and described. We have shown that, for frozen samples mRNA detection is optimal within 3 months of the biopsy being taken, while preservation declines after 1 year of storage.
Pitera
R
R
Department of Histopathology, King's College School of Medicine and Dentistry, London, England.
Pitera
J E
JE
Salisbury
J R
JR
D001706:Biopsy; D005069:Evaluation Studies as Topic; D005629:Frozen Sections; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods*; D008198:Lymph Nodes / Q000737:chemistry; D008223:Lymphoma / Q000235:genetics*; D011309:Preservation, Biological; D012333:RNA, Messenger / Q000032:analysis*; D013997:Time Factors; D014158:Transcription, Genetic
D012333:RNA, Messenger