The O:34-antigen lipopolysaccharide as an adhesin in Aeromonas hydrophila.
We compared the ability of different Aeromonas hydrophila strains from serogroup O:34 grown at different temperatures to adhere to Hep-2 cells. We found a high level of adhesion when the strains were grown at 20 degrees C but not when they were grown at 37 degrees C. We previously described that these strains were able to form the O-antigen lipopolysaccharide when they grow at low temperature but not at high temperature. We also obtained by transposon mutagenesis mutants only devoid of the O-antigen lipopolysaccharide (rfb mutants), and they showed significantly lower levels of adhesion to Hep-2 cells than the smooth strains. All these results prompted us to conclude that the O-antigen LPS, in these strains, is an important adhesin.
Merino
S
S
Departamento de Microbiología, Facultad de Biología, Universidad de Barcelona, Spain.
Rubires
X
X
Aguilar
A
A
Tomás
D016980:Aeromonas hydrophila / Q000166:cytology / Q000235:genetics*; D000941:Antigens / Q000502:physiology*; D001422:Bacterial Adhesion / Q000502:physiology; D002448:Cell Adhesion / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008070:Lipopolysaccharides / Q000276:immunology / Q000378:metabolism*; D016296:Mutagenesis / Q000502:physiology; D014407:Tumor Cells, Cultured
D000941:Antigens; D008070:Lipopolysaccharides