title: Subunit orientation in the filamentous virus Ff(fd, f1, M13).
abstract: The Ff filamentous viruses (fd, M13, f1) are important models for membrane protein assembly and are used extensively as cloning vectors and vehicles for peptide display. The thread-like virion (approximately 6 nm x 880 nm) comprises a single-stranded DNA genome sheathed by approximately 2700 copies of a 50-residue alpha-helical subunit (pVIII). The average inclination of pVIII subunits from the virion axis has been determined experimentally by polarized Raman microspectroscopy of oriented fd fibers, using the amide I Raman tensors obtained from a peptide single crystal. It is found that the average tilt angle, theta, between the pVIII alpha-helix and the virion axis falls within the range 13< theta <20 degrees.
authors.affiliation: Division of Cell Biology and Biophysics, School of Biological Sciences, University of Missouri-Kansas City, 64110, USA.
authors.forename: S A
authors.initials: SA
authors.lastname: Overman
authors.forename: M
authors.initials: M
authors.lastname: Tsuboi
authors.forename: G J
authors.initials: GJ
authors.lastname: Thomas
mesh_terms: D017104:Bacteriophage M13 / Q000648:ultrastructure; D017912:Inovirus / Q000378:metabolism / Q000648:ultrastructure*; D008958:Models, Molecular; D017433:Protein Structure, Secondary; D013059:Spectrum Analysis, Raman / Q000295:instrumentation / Q000379:methods; D014758:Viral Core Proteins / Q000737:chemistry*; D014771:Virion / Q000737:chemistry / Q000648:ultrastructure
chemical_list: D014758:Viral Core Proteins