[Structure of a new lysine-containing O-specific polysaccharide from Proteus mirabilis O26].
The composition and structure of the O-specific polysaccharide chain of the bacterium Proteus mirabilis O26 lipopolysaccharide have been studied using one- and two-dimensional 1H- and 13C-NMR spectroscopy, including homonuclear correlation spectroscopy (COSY), H-detected 1H,13C heteronuclear multi-quantum coherence and rotating-frame nuclear Overhauser effect spectroscopy. It has been found that the polysaccharide is acidic due to the presence of D-galacturonic acid (D-GalA) residues, part of which are O-acetylated, while the other part form an amide with the alpha-amino group of L-lysine and is built up of tetrasaccharide repeating units having the following structure: [formula: see text] The P. mirabilis O26 polysaccharide is structurally similar to the O-specific polysaccharide of P. mirabilis O28 studied earlier by us and containing amides of D-galacturonic acid with L-lysine and L-serine.
Shashkov
A S
AS
N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow.
Toukach
F V
FV
Paramonov
N A
NA
Senchenkova
S N
SN
Katsa
V
V
Knirel'
Iu A
IuA
D000577:Amides / Q000737:chemistry; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D006603:Hexuronic Acids / Q000737:chemistry; D008239:Lysine / Q000032:analysis*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D019081:O Antigens / Q000737:chemistry*; D011513:Proteus mirabilis / Q000737:chemistry*
D000577:Amides; D006603:Hexuronic Acids; D019081:O Antigens; C007819:galacturonic acid; D008239:Lysine
Struktura novogo lizinsoderzhashchego O-spetsificheskogo polisakharida bakterii Proteus mirabilis O26.