title: Genomic DNA analysis from mouse toe lysates.
abstract: The protocol described in this report provides a simple, accurate and efficient assay for detection of transgenes and mutations in large colonies of rodents, using crude lysates prepared from the digit cut from the animals for identification purposes. This can be done as early as 6 days of age, minimizing trauma to the mice and allowing assays to be completed long before weaning.
authors.affiliation: Department of Biological Sciences, University of Tulsa, OK 74104, USA.
authors.forename: N L
authors.initials: NL
authors.lastname: Nadon
authors.forename: K
authors.initials: K
authors.lastname: Draeger
mesh_terms: D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000032:analysis* / Q000302:isolation & purification; D004252:DNA Mutational Analysis; D016678:Genome; D051379:Mice / Q000235:genetics*; D008816:Mice, Jimpy; D008817:Mice, Mutant Strains / Q000235:genetics*; D008822:Mice, Transgenic / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D018991:Myelin Proteolipid Protein / Q000235:genetics; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D014034:Toes; D019076:Transgenes
chemical_list: D018991:Myelin Proteolipid Protein; D004247:DNA