title: Pore formation by S. aureus alpha-toxin in liposomes and planar lipid bilayers: effects of nonelectrolytes.
abstract: Nonelectrolytes such as polyethylene glycols (PEG) and dextrans (i) promote the association of S. aureus alpha-toxin with liposomes (shown by Coomassie staining) and (ii) enhance the rate and extent of calcein leakage from calcein-loaded liposomes; such leakage is inhibited by H+, Zn2+ and Ca2+ to the same extent as that of nonPEG-treated liposomes. Incubation of liposomes treated with alpha-toxin in the presence of PEG with the hydrophobic photo-affinity probe 3-(trifluoromethyl)-3-m-[125I]iodophenyl) diazirine (125I-TID) labels monomeric and-predominantly-hexameric forms of liposome-associated alpha-toxin; in the absence of PEG little labeling is apparent. At high concentrations of H+ and Zn2+ but not of Ca(2+)-all of which inhibit calcein leakage-the distribution of label between hexamer and monomer is perturbed in favor of the latter. In alpha-toxin-treated planar lipid bilayers from which excess toxin has been washed away, PEGs and dextrans strongly promote the appearance of ion-conducting pores. The properties of such pores are similar in most regards to pores induced in the absence of nonelectrolytes; the differ only in being more sensitive to "closure" by voltage (as are pores induced in cells). In both systems, the stimulation by nonelectrolytes increase with concentration and with molecular mass up to a maximum around 2,000 Da. We conclude (i) that most of the alpha toxin that becomes associated with liposome or planar lipid bilayers does not form active pores and (ii) that the properties of alpha-toxin-induced pores in lipid bilayers can be modulated to resemble those in cells.
authors.affiliation: Department of Cellular & Molecular Sciences, St George's Hospital Medical School, London, UK.
authors.forename: C L
authors.initials: CL
authors.lastname: Bashford
authors.forename: G M
authors.initials: GM
authors.lastname: Alder
authors.forename: L G
authors.initials: LG
authors.lastname: Fulford
authors.forename: Y E
authors.initials: YE
authors.lastname: Korchev
authors.forename: E
authors.initials: E
authors.lastname: Kovacs
authors.forename: A
authors.initials: A
authors.lastname: MacKinnon
authors.forename: C
authors.initials: C
authors.lastname: Pederzolli
authors.forename: C A
authors.initials: CA
authors.lastname: Pasternak
mesh_terms: D000818:Animals; D001427:Bacterial Toxins / Q000737:chemistry* / Q000633:toxicity*; D003911:Dextrans / Q000494:pharmacology; D004912:Erythrocytes / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D005452:Fluoresceins / Q000493:pharmacokinetics; D006460:Hemolysin Proteins / Q000737:chemistry* / Q000633:toxicity*; D006461:Hemolysis / Q000187:drug effects; D066298:In Vitro Techniques; D007473:Ion Channels / Q000737:chemistry / Q000187:drug effects; D008051:Lipid Bilayers / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000494:pharmacology; D011817:Rabbits; D013211:Staphylococcus aureus
chemical_list: D001427:Bacterial Toxins; D003911:Dextrans; D005452:Fluoresceins; D006460:Hemolysin Proteins; D007473:Ion Channels; D008051:Lipid Bilayers; D008081:Liposomes; C021091:staphylococcal alpha-toxin; D011092:Polyethylene Glycols; C007740:fluorexon