A novel method for studying preadipocyte differentiation in vitro.
In vitro differentiation of rat preadipocytes has typically been induced in medium supplemented with pharmacological concentrations of hormonal mixtures. These conditions probably do not reflect the milieu within adipose tissue in vivo. We have developed a new method for inducing differentiation of preadipocytes using culture medium which has been conditioned by isolated adipocytes (ACM). In the presence of ACM, 70%-80% of test preadipocytes contained lipid inclusions compared to < 5% of control. When differentiation was assessed by assay of glycerol-3-phosphate dehydrogenase activity, ACM activity was shown to be reproducible and the consistency of response to ACM by different pools of preadipocytes was comparable to that induced by standard differentiation procedures. We have also demonstrated that the adipogenic activity of ACM may not depend on prostaglandin secretion by adipocytes. We propose that use of paracrine factors produced by components of adipose tissue provides a new approach to preadipocyte differentiation induction which may more closely reproduce the adipose tissue environment.
Shillabeer
G
G
Department of Medicine, University of Ottawa, Ontario, Canada.
Li
Z H
ZH
Hatch
G
G
Kumar
V
V
Lau
D C
DC
D000241:Adenosine / Q000494:pharmacology; D017667:Adipocytes / Q000166:cytology* / Q000378:metabolism; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D017077:Culture Media, Conditioned; D005993:Glycerolphosphate Dehydrogenase / Q000378:metabolism; D007213:Indomethacin / Q000494:pharmacology; D007700:Kinetics; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D013234:Stem Cells / Q000166:cytology*; D014280:Triglycerides / Q000378:metabolism
D017077:Culture Media, Conditioned; D014280:Triglycerides; D005993:Glycerolphosphate Dehydrogenase; D000241:Adenosine; D007213:Indomethacin