Slow induction of gelatinase B mRNA by acidic culture conditions in mouse metastatic melanoma cells.
Gelatinase B has been thought to be a key enzyme for degradation of extracellular matrix in tumour invasion and metastasis. In this study, we examined the effect of acidic culture medium (pH 5.9) on the expression of gelatinase B mRNA in mouse metastatic melanoma cell line (B16-F10). Using reverse transcription-polymerase chain reaction (RT-PCR) analysis, we found that gelatinase B was induced by the acidic culture medium at 24 h, and then gradually diminished to 72 h. By gelatin zymographic analysis, gelatinase B was first detected at 24 h, continued to increase and then reached a plateau at around 48 h. These results suggest that the induction of gelatinase B secretion by acidic culture medium occurs as a result of the gene expression.
Kato
Y
Y
Department of Oral Biochemistry, Kanagawa Dental College, Yokosuka, Japan.
Ozono
S
S
Shuin
T
T
Miyazaki
K
K
D000818:Animals; D001483:Base Sequence; D017364:Collagenases / Q000235:genetics* / Q000378:metabolism; D003470:Culture Media / Q000494:pharmacology; D007773:Lactates / Q000494:pharmacology*; D019344:Lactic Acid; D020780:Matrix Metalloproteinase 9; D008545:Melanoma / Q000556:secondary; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism; D014158:Transcription, Genetic / Q000187:drug effects / Q000235:genetics; D014407:Tumor Cells, Cultured / Q000166:cytology / Q000187:drug effects / Q000201:enzymology
D003470:Culture Media; D007773:Lactates; D012333:RNA, Messenger; D019344:Lactic Acid; D017364:Collagenases; D020780:Matrix Metalloproteinase 9