Establishment of AIDS-related lymphoma cell lines from lymphomatous effusions.
AIDS-related non-Hodgkin lymphomas (AIDS-NHL) are most frequently derived from B cells and include small non-cleaved cell lymphoma (SNCCL) and diffuse large cell lymphoma (DLCL) and less frequently anaplastic large cell lymphoma (ALCL) or body cavity-based lymphoma (BCBL). AIDS-NHL cell lines have proved useful to study AIDS-NHL pathogenesis. In this report, we describe the establishment and molecular characterization of two novel AIDS-NHL cell lines (HBL-4 and HBL-6) derived from lymphomatous effusions. HBL-4 was derived from a patient with SNCCL, whereas HBL-6 was derived from a patient with BCBL. The identity of the cell lines with the original tumor clone was established by immunoglobulin gene rearrangement analysis. Both HBL-4 and HBL-6 carry a monoclonal EBV infection and do not contain HIV. In addition, HBL-6 harbors DNA sequences of the recently identified Kaposi's sarcoma-associated herpesvirus (KSHV), now formally called human herpesvirus 8 (HHV8). Finally, HBL-4, but not HBL-6, harbors a rearranged c-MYC allele, while the BCL-6 gene displayed a germline configurations in both cell lines. These AIDS-NHL cell lines may prove useful in understanding the biologic events contributing to AIDS-NHL development.
Gaidano
G
G
Department of Pathology, College of Physicians and Surgeons, Columbia University, New York, USA.
Cechova
K
K
Chang
Y
Y
Moore
P S
PS
Knowles
D M
DM
Dalla-Favera
R
R
D001202:Ascitic Fluid / Q000473:pathology*; D001483:Base Sequence; D015321:Gene Rearrangement; D015322:Gene Rearrangement, B-Lymphocyte; D016259:Genes, myc; D006564:Herpesviridae / Q000302:isolation & purification; D004854:Herpesvirus 4, Human / Q000302:isolation & purification; D006801:Humans; D016483:Lymphoma, AIDS-Related / Q000235:genetics / Q000473:pathology* / Q000821:virology; D008228:Lymphoma, Non-Hodgkin / Q000235:genetics / Q000473:pathology / Q000821:virology; D008969:Molecular Sequence Data; D014407:Tumor Cells, Cultured