Cloning and characterization of cDNAs encoding two forms of avian stem cell factor.
Stem cell factor (SCF), also known as Steel factor, is a transmembrane cytokine involved in several developmental processes and the ligand for the receptor tyrosine kinase c-kit. In several mammalian species, two isoforms of stem cell factor have been reported, a long form in which soluble SCF is released after proteolytic cleavage of the extracellular domain and a short membrane-anchored form in which a region containing a cleavage site is deleted. Currently, only the longer, soluble form has been identified in birds. Therefore, the cDNAs encoding two forms of quail stem cell factor (qSCF) were obtained using RT-PCR with nested primers. The deduced amino acid sequence of the long form of qSCF showed a high degree of homology with chicken (98%) and relatively low homology (approximately 53%) with various mammalian SCFs. Northern blot analysis with the qSCF cDNA revealed the expression of a 5.9 and a 2.7 kb transcript in several quail tissues.
Petitte
J N
JN
Department of Poultry Science, North Carolina State University, Raleigh 27695-7608, USA. j_petitte@ncsu.edu
Kulik
M J
MJ
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002645:Chickens; D003001:Cloning, Molecular; D018076:DNA, Complementary; D008969:Molecular Sequence Data; D011784:Quail; D017386:Sequence Homology, Amino Acid; D019089:Stem Cell Factor / Q000235:genetics*
D018076:DNA, Complementary; D019089:Stem Cell Factor