Seven genes from human chromosome 18 map to chromosome 24 in the bovine.
Bovine sequence tagged sites (STSs) were developed for seven genes and used for synteny mapping with a hybrid bovine x rodent cell line panel. The genes were thymidylate synthase (TYMS), pituitary adenylate cyclase activating peptide (ADCYAP1), and melanocortin-2 receptor (MC2R) from the short arm of human chromosome (HSA) 18 and N-cadherin (CDH2), transthyretin (TTR), gastrin-releasing peptide (GRP), and plasminogen activator inhibitor 2 (PAI2) from the long arm of HSA 18. Primers for these genes were designed with human, ovine, or bovine sequences aligned with a sequence from a second species. The bovine PCR product was cloned, and the fragment was sequenced to verify that the homologous gene was indeed amplified. A second set of bovine-specific PCR primers were developed for each gene from these sequences. These STSs were used for synteny mapping, and all seven genes were syntenic with markers of bovine chromosome (BTA) 24. The concordance with BTA 24 was at least 96.5% for all genes.
Larsen
N J
NJ
Department of Animal Sciences, University of Wisconsin, Madison 53706, USA.
Womack
J E
JE
Kirkpatrick
B W
BW
D000818:Animals; D001483:Base Sequence; D015820:Cadherins / Q000235:genetics; D002417:Cattle / Q000235:genetics*; D002874:Chromosome Mapping; D002887:Chromosomes, Human, Pair 18; D017931:DNA Primers; D019886:Gastrin-Releasing Peptide; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000235:genetics; D010455:Peptides / Q000235:genetics; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; D017396:Plasminogen Activator Inhibitor 2 / Q000235:genetics; D011228:Prealbumin / Q000235:genetics; D051381:Rats; D018043:Receptors, Corticotropin / Q000235:genetics; D044101:Receptors, Melanocortin; D016324:Sequence Tagged Sites; D012756:Sheep; D013940:Thymidylate Synthase / Q000235:genetics
C495401:ADCYAP1 protein, human; C495402:Adcyap1 protein, mouse; C495403:Adcyap1 protein, rat; D015820:Cadherins; D017931:DNA Primers; D009479:Neuropeptides; D010455:Peptides; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; D017396:Plasminogen Activator Inhibitor 2; D011228:Prealbumin; D018043:Receptors, Corticotropin; D044101:Receptors, Melanocortin; D019886:Gastrin-Releasing Peptide; D013940:Thymidylate Synthase