Resuscitation of cadaveric livers from non-heart-beating donors after warm ischemic insult: a novel technique tested in the rat.
Clinical liver transplantation has become the therapy of choice in end-stage liver disease, but the limited availability of suitable donor organs still impedes its widespread application. In order to increase the availability of donor organs for liver transplantation, it would be advantageous if ischemically damaged livers could be resuscitated from cadavers in which the heart has stopped beating. A method for doing this has been developed in a rat model. Compared to livers excised from rats in which the heart is still beating, severe deteriorations of tissue integrity and functional performance were evident in predamaged livers after cold preservation without supplementary treatment. A treatment of those livers which included an antioxidant rinse with superoxide dismutase, and venous vascular insufflation of gaseous oxygen during preservation, completely prevented tissue alterations upon reperfusion, and promoted a functional recovery of the livers, making them comparable to organs harvested from heart-beating donors.
Minor
T
T
Institute for Experimental Medicine, University of Cologne, Germany.
Klauke
H
H
Isselhard
W
W
D000227:Adenine Nucleotides / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001646:Bile / Q000378:metabolism; D002102:Cadaver; D004563:Electrochemistry; D004734:Energy Metabolism; D006358:Hot Temperature; D007511:Ischemia; D015227:Lipid Peroxidation; D008099:Liver / Q000098:blood supply* / Q000502:physiology; D016031:Liver Transplantation; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D014019:Tissue Donors; D014021:Tissue Preservation
D000227:Adenine Nucleotides; D008315:Malondialdehyde; D000255:Adenosine Triphosphate