New vectors for peptide display on the surface of filamentous bacteriophage.
We have modified the genome of the filamentous bacteriophage fd and also constructed a number of new vectors for the purpose of displaying peptides on the surface of the virion. These vectors facilitate the directional cloning of DNA encoding a peptide of interest at or near the N terminus of the major coat protein, the product of the bacteriophage gene VIII, and the construction of hybrid capsids in which the modified coat protein is interspersed with wild-type coat protein subunits.
Malik
P
P
Cambridge Centre for Molecular Recognition, Department of Biochemistry, University of Cambridge, UK. pm@mole.bio.cam.ac.uk
Perham
R N
RN
D000595:Amino Acid Sequence; D001483:Base Sequence; D002213:Capsid / Q000096:biosynthesis / Q000235:genetics; D003001:Cloning, Molecular / Q000379:methods; D005822:Genetic Vectors / Q000235:genetics*; D016679:Genome, Viral; D017912:Inovirus / Q000235:genetics*; D008969:Molecular Sequence Data; D010455:Peptides / Q000235:genetics
D010455:Peptides