Complementation of an Arabidopsis thaliana biotin auxotroph with an Escherichia coli biotin biosynthetic gene.
The Arabidopsis thaliana biotin auxotroph biol was rendered prototrophic by transformation with a chimeric transgene containing the Escherichia coli bio A gene driven by a constitutive promoter. The bio A gene encodes the biotin biosynthetic enzyme 7, 8-diaminopelargonic acid aminotransferase. Unlike the untransformed control plants, transgenic plants expressing the bacterial transgene synthesized biotin and grew to maturity without biotin-deficiency symptoms. These findings demonstrate that bio1/bio1 mutant plants are defective in the gene encoding 7,8-diaminopelargonic acid aminotransferase.
Patton
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Ciba Agricultural Biotechnology, Research Triangle Park, NC 27709, USA.
Volrath
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Ward
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D000599:Amino Acids, Diamino / Q000235:genetics*; D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development; D001483:Base Sequence; D001710:Biotin / Q000096:biosynthesis*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D015870:Gene Expression; D005798:Genes, Bacterial / Q000235:genetics; D017343:Genes, Plant / Q000235:genetics; D008969:Molecular Sequence Data; D009154:Mutation; D030821:Plants, Genetically Modified / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D018749:RNA, Plant / Q000032:analysis; D019076:Transgenes / Q000235:genetics
D000599:Amino Acids, Diamino; D012333:RNA, Messenger; D018749:RNA, Plant; C004720:7,8-diaminopelargonic acid; D001710:Biotin