The tomato Dwarf gene isolated by heterologous transposon tagging encodes the first member of a new cytochrome P450 family.
To transposon tag the tomato Dwarf (D) gene, a tomato line that carries a T-DNA containing a maize Activator (Ac) transposable element closely linked to D was pollinated with a stock homozygous for the d mutation. Hybrid seedlings were screened for dwarf progeny, and three independent dwarf lines were obtained. Two of these lines showed inheritance of a recessive phenotype similar to that conferred by the extreme dwarf (dx) allele. Variegation for the dwarf phenotype in one of these lines suggested that D had been tagged by Ac. Genomic DNA adjacent to Ac in these two lines was isolated by use of the inverse polymerase chain reaction, and the two insertions mapped approximately 2 kb apart. Partial complementation of d was observed when the corresponding wild-type sequence was used in transformation experiments. A cDNA clone of D was sequenced, and the predicted amino acid sequence has homology to cytochrome P450 enzymes.
Bishop
G J
GJ
Sainsbury Laboratory, John Innes Centre, Norwich, United Kingdom.
Harrison
K
K
Jones
J D
JD
D000483:Alleles; D000595:Amino Acid Sequence; D001483:Base Sequence; D017124:Conserved Sequence; D003433:Crosses, Genetic; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis* / Q000235:genetics; D017931:DNA Primers; D004251:DNA Transposable Elements; D018076:DNA, Complementary; D018744:DNA, Plant / Q000302:isolation & purification*; D017343:Genes, Plant; D005808:Genes, Recessive; D018551:Solanum lycopersicum / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D005810:Multigene Family; D016254:Mutagenesis, Insertional; D010641:Phenotype; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D003313:Zea mays / Q000235:genetics
D017931:DNA Primers; D004251:DNA Transposable Elements; D018076:DNA, Complementary; D018744:DNA, Plant; D003577:Cytochrome P-450 Enzyme System