Hexose recognition by insulin-secreting BRIN-BD11 cells.
Clonal BRIN-BD11 cells were produced by electrofusion of NEDH rat islet B-cells with immortal RINm5F cells. Western blotting analysis revealed that unlike RINm5F, novel BRIN-BD11 cells expressed high levels of the glucose transport protein GLUT-2, coupled with a rapid and sustained uptake of D-glucose, significantly greater than RINm5F after only 5 min (p < 0.05). Whereas BRIN-BD11 cells expressed a high glucokinase:hexokinase ratio with 1.4-2.0 fold and 1.4-1.7 fold stepwise stimulation of insulin secretion with 4.2-16.7 mM D-glucose and D-mannose respectively, RINm5F had a lower glucokinase:hexokinase ratio (p < 0.001) and were notably unresponsive to D-glucose and D-mannose. Unlike RINm5F cells, BRIN-BD11 were unresponsive to other hexoses, with RINm5F only responding to D-galactose (p < 0.05). BRIN-BD11 cells should be useful for studies of nutrient-induced insulin secretion.
McClenaghan
N H
NH
School of Biomedical Sciences, University of Ulster, Coleraine, United Kingdom.
Gray
A M
AM
Barnett
C R
CR
Flatt
P R
PR
D000818:Animals; D002459:Cell Fusion; D002460:Cell Line; D002999:Clone Cells; D003847:Deoxyglucose / Q000378:metabolism; D005941:Glucokinase / Q000096:biosynthesis; D005947:Glucose / Q000378:metabolism* / Q000494:pharmacology; D006593:Hexokinase / Q000096:biosynthesis; D006601:Hexoses / Q000494:pharmacology*; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007340:Insulinoma; D007515:Islets of Langerhans / Q000187:drug effects / Q000378:metabolism*; D007700:Kinetics; D008358:Mannose / Q000494:pharmacology; D010190:Pancreatic Neoplasms; D051381:Rats
D006601:Hexoses; D007328:Insulin; D003847:Deoxyglucose; D006593:Hexokinase; D005941:Glucokinase; D005947:Glucose; D008358:Mannose