Engineered RNA-mediated resistance to tomato spotted wilt virus is sequence specific.
Transgenic plants were produced that expressed a wide range of randomly chosen sequences of the tripartite tomato spotted wilt virus (TSWV) RNA genome or its complement. Testing the progenies of these plants revealed that only transgenic expression of N or NS(M) gene sequences resulted in resistance to TSWV.
Prins
M
M
Department of Virology, Wageningen Agricultural University, Wageningen; The Netherlands.
Resende
R de O
Rde O
Anker
C
C
van Schepen
A
A
de Haan
P
P
Goldbach
R
R
D002213:Capsid / Q000096:biosynthesis; D016679:Genome, Viral; D007113:Immunity, Innate; D018551:Solanum lycopersicum / Q000235:genetics / Q000821:virology*; D010935:Plant Diseases; D010942:Plant Viruses / Q000235:genetics* / Q000472:pathogenicity*; D030821:Plants, Genetically Modified; D010947:Plants, Toxic; D012328:RNA Viruses / Q000235:genetics / Q000472:pathogenicity; D012367:RNA, Viral / Q000096:biosynthesis*; D014026:Nicotiana; D014158:Transcription, Genetic; D014758:Viral Core Proteins / Q000096:biosynthesis; D017361:Viral Nonstructural Proteins / Q000096:biosynthesis
D012367:RNA, Viral; D014758:Viral Core Proteins; D017361:Viral Nonstructural Proteins