Glutathione depletion induces glycogenolysis dependent ascorbate synthesis in isolated murine hepatocytes.
The relationship between glutathione deficiency, glycogen metabolism and ascorbate synthesis was investigated in isolated murine hepatocytes. Glutathione deficiency caused by various agents increased ascorbate synthesis with a stimulation of glycogen breakdown. Increased ascorbate synthesis from UDP-glucose or gulonolactone could not be further affected by glutathione depletion. Fructose prevented the stimulated glycogenolysis and ascorbate synthesis caused by glutathione consumption. Reduction of oxidised glutathione by dithiothreitol decreased the elevated glycogenolysis and ascorbate synthesis in diamide or menadione treated hepatocytes. Our results suggest that a change in GSH/GSSG ratio seems to be a sufficient precondition of altering glycogenolysis and a consequent ascorbate synthesis.
Braun
L
L
Department of Medical Chemistry, Semmelweis University of Medicine, Budapest, Hungary.
Csala
M
M
Poussu
A
A
Garzó
T
T
Mandl
J
J
Bánhegyi
G
G
D000082:Acetaminophen / Q000494:pharmacology; D000818:Animals; D001205:Ascorbic Acid / Q000096:biosynthesis*; D003994:Bucladesine / Q000494:pharmacology; D019328:Buthionine Sulfoximine; D000242:Cyclic AMP / Q000378:metabolism; D003958:Diamide / Q000494:pharmacology; D005632:Fructose / Q000494:pharmacology; D005978:Glutathione / Q000172:deficiency / Q000378:metabolism*; D006003:Glycogen / Q000378:metabolism*; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008717:Methionine Sulfoximine / Q000031:analogs & derivatives / Q000494:pharmacology; D051379:Mice; D013400:Sugar Acids / Q000494:pharmacology; D014532:Uridine Diphosphate Glucose; D014812:Vitamin K / Q000494:pharmacology
D013400:Sugar Acids; D003958:Diamide; D014812:Vitamin K; D008717:Methionine Sulfoximine; D005632:Fructose; C007895:gulonolactone; D000082:Acetaminophen; D019328:Buthionine Sulfoximine; D003994:Bucladesine; D006003:Glycogen; D000242:Cyclic AMP; D005978:Glutathione; D001205:Ascorbic Acid; D014532:Uridine Diphosphate Glucose