Chromatographic resolution of partially perdeuterated diglucosyldiacylglycerols from Acholeplasma laidlawii.
Diglucosyldiacylglycerol from Acholeplasma laidlawii was isolated by adsorption chromatography and subsequently subjected to molecular species analysis by reversed-phase high-performance liquid chromatography. Twenty-three molecular species were resolved. The bacterium was grown in a medium supplemented with a mixture of oleic acid and perdeuterated palmitic acid, and incorporation of perdeuterated acyl moieties occurred in several molecular species. Short and odd-numbered acyl chains were found in combinations with palmitic acid, perdeuterated palmitic acid and oleic acid. Also, molecular species differing only by perdeuterated and protonated acyl chain content were resolved chromatographically. Separation between diglucosyldiacylglycerol and digalactosyldiacylglycerol isolated from a plant source was achieved with adsorption chromatography. The polarity of diglucosyldiacylglycerol as compared to digalactosyldiacylglycerol is discussed.
Bergqvist
M H
MH
Department of Analytical Chemistry, Stockholm University, Sweden.
D000128:Acholeplasma laidlawii / Q000737:chemistry*; D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003903:Deuterium; D004075:Diglycerides / Q000737:chemistry / Q000302:isolation & purification*; D013058:Mass Spectrometry; D008969:Molecular Sequence Data
D004075:Diglycerides; D003903:Deuterium