Role of granulocyte elastase in the formation of hemorrhagic shock-induced gastric mucosal lesions in the rat.
OBJECTIVE
To investigate the mechanism by which activated leukocytes induce gastric mucosal lesions, we examined whether granulocyte elastase is involved in the formation of such lesions in a rat model of hemorrhagic shock.


METHODS
Prospective, randomized, controlled study.


METHODS
Research laboratory at a university medical center.


METHODS
Male Wistar rats, weighing 220 to 280 g.


METHODS
Animals were subjected to hemorrhagic shock by phlebotomy. ONO-5046, a granulocyte elastase inhibitor (300 mg/kg ip), was administered 30 mins before or after phlebotomy. The effects of antithrombotic substances and tranexamic acid on hemorrhagic shock-induced gastric mucosal lesions also were examined. The effects of granulocyte elastase on the thrombomodulin activity and 35S-glycosaminoglycan content of endothelial cells were examined, using cultured human umbilical vein endothelial cells.
Kushimoto
S
S
Department of Laboratory Medicine, Kumamoto University Medical School, Japan.
Okajima
K
K
Okabe
H
H
Binder
B R
BR
D000818:Animals; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000201:enzymology; D005753:Gastric Mucosa / Q000187:drug effects / Q000503:physiopathology*; D005998:Glycine / Q000031:analogs & derivatives / Q000494:pharmacology / Q000627:therapeutic use; D006801:Humans; D019272:Leukocyte Elastase; D008297:Male; D010196:Pancreatic Elastase / Q000037:antagonists & inhibitors / Q000502:physiology*; D018962:Phlebotomy; D011446:Prospective Studies; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D015842:Serine Proteinase Inhibitors / Q000494:pharmacology / Q000627:therapeutic use; D012771:Shock, Hemorrhagic / Q000150:complications*; D013276:Stomach Ulcer / Q000209:etiology* / Q000517:prevention & control; D013449:Sulfonamides / Q000494:pharmacology / Q000627:therapeutic use
D015842:Serine Proteinase Inhibitors; D013449:Sulfonamides; C069195:sivelestat; D010196:Pancreatic Elastase; D019272:Leukocyte Elastase; D005998:Glycine