title: Overexpression of the proto-oncogene c-jun in association with low-risk type specific human papillomavirus infection in condyloma acuminata.
abstract: Infection with different types of human papillomavirus (HPV) is associated with neoplasia at different anatomic sites. The "low-risk" HPVs (LR-HPV) are responsible for benign genital lesions such as condyloma acuminata. In order to clarify the tumorigenic mechanism of LR-HPV, the HPV infection status was investigated and the expression of the c-jun proto-oncogene in different HPV-related skin and genital lesions analyzed. Of the 17 condyloma specimens analyzed by Western blotting, 13 cases (76.5%) exhibited overexpression of the c-jun gene. All 13 cases harbored high copy numbers of the LR-HPV genome with an average of 926 copies per cell, whereas the other four cases had an average of 12 copies of LR-HPV per cell (P < 0.001). Further typing of HPV by Southern blotting revealed that HPV-6 and HPV-11 infections predominated in c-jun positive cases. The c-jun protein was detected much less frequently in cervical cancers (three of 29, or 10.3%) and skin warts (one of 10), and was not detected in five genital polyps or in five normal cervical tissues. These findings suggest a type 6/11-specific induction of c-jun gene expression in HPV-related neoplastic lesions.
authors.affiliation: Department of Obstetrics and Gynecology, Tri-Service General Hospital, Taipei, Taiwan, Republic of China.
authors.forename: Y F
authors.initials: YF
authors.lastname: Yang
authors.forename: Y P
authors.initials: YP
authors.lastname: Tsao
authors.forename: C S
authors.initials: CS
authors.lastname: Yin
authors.forename: S L
authors.initials: SL
authors.lastname: Chen
authors.forename: T Y
authors.initials: TY
authors.lastname: Chu
mesh_terms: D000818:Animals; D003218:Condylomata Acuminata / Q000378:metabolism* / Q000821:virology; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D015870:Gene Expression; D006801:Humans; D051379:Mice; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000378:metabolism* / Q000821:virology; D000090063:Proto-Oncogene Mas; D016755:Proto-Oncogene Proteins c-jun / Q000378:metabolism*; D012307:Risk Factors; D002583:Uterine Cervical Neoplasms / Q000378:metabolism / Q000821:virology*
chemical_list: D004279:DNA, Viral; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D016755:Proto-Oncogene Proteins c-jun