Suppression of interleukin-2 receptor expression on mouse CD4+ T cells by bovine kappa-caseinoglycopeptide.
Bovine kappa-caseinoglycopeptide (residues 106-169, CGP) completely inhibited the PHA-induced proliferation of mouse splenocytes when CGP was added simultaneously with PHA. The inhibitory effect, however, was reduced to about one-half when CGP was added after 24 h of cultivating the splenocytes with PHA or when anti-IL-1ra antibody was added simultaneously with PHA and CGP. On the other hand, CGP bound to mouse CD4+ T cells but not to CD8+ T cells. CGP suppressed IL-2 receptor expression of the PHA-stimulated mouse CD4+ T cells.
Otani
H
H
Division of Animal Science, Faculty of Agriculture, Shinshu University, Nagano, Japan.
Horimoto
Y
Y
Monnai
M
M
D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D002364:Caseins / Q000494:pharmacology*; D002417:Cattle; D002478:Cells, Cultured; D006020:Glycopeptides / Q000494:pharmacology*; D006801:Humans; D051379:Mice; D008809:Mice, Inbred C3H; D011817:Rabbits; D017472:Receptors, Interleukin-1 / Q000037:antagonists & inhibitors; D015375:Receptors, Interleukin-2 / Q000096:biosynthesis* / Q000187:drug effects; D013154:Spleen / Q000166:cytology
D002364:Caseins; D006020:Glycopeptides; D017472:Receptors, Interleukin-1; D015375:Receptors, Interleukin-2; C094310:kappa-caseinoglycopeptide