Evidence that biliverdin-IX beta reductase and flavin reductase are identical.
A search of the database shows that human biliverdin-IX beta reductase and flavin reductase are identical. We have isolated flavin reductase from bovine erythrocytes and show that the activity co-elutes with biliverdin-IX beta reductase. Preparations of the enzyme that are electrophoretically homogeneous exhibit both flavin reductase and biliverdin-IX beta reductase activities; however, they are not capable of catalysing the reduction of biliverdin-IX alpha. Although there is little obvious sequence identity between biliverdin-IX alpha reductase (BVR-A) and biliverdin-IX beta reductase (BVR-B), they do show weak immunological cross-reactivity. Both enzymes bind to 2',5'-ADP-Sepharose.
Shalloe
F
F
Department of Biochemistry, Trinity College, Dublin, Ireland.
Elliott
G
G
Ennis
O
O
Mantle
T J
TJ
D000595:Amino Acid Sequence; D000818:Animals; D001664:Biliverdine / Q000378:metabolism*; D002417:Cattle; D002850:Chromatography, Gel; D004912:Erythrocytes / Q000201:enzymology*; D038181:FMN Reductase; D006801:Humans; D008969:Molecular Sequence Data; D009247:NADH, NADPH Oxidoreductases / Q000737:chemistry*; D010088:Oxidoreductases / Q000737:chemistry*; D044925:Oxidoreductases Acting on CH-CH Group Donors; D010446:Peptide Fragments / Q000737:chemistry; D016415:Sequence Alignment; D017421:Sequence Analysis
D010446:Peptide Fragments; D010088:Oxidoreductases; D044925:Oxidoreductases Acting on CH-CH Group Donors; C019789:biliverdin reductase; D038181:FMN Reductase; D009247:NADH, NADPH Oxidoreductases; D001664:Biliverdine