Fast atom bombardment and electrospray ionization tandem mass spectrometry of sulfated Lewis(x) trisaccharides.
Structural characterization of mono-, di-, and trisulfated trisaccharides by fast atom bombardment (FAB) and electrospray ionization (ESI) mass spectrometry is described. Both FAB and ESI collision-induced dissociation tandem mass spectrometry (CID-MS/MS) of sulfated trisaccharides allowed identification of the sulfated sugar and provided information on the saccharide chain sequence. The ESI CID-MS/MS of multiple-charged ions provided even more structural information, and some of the useful daughter ions appeared in the higher mass region than the precursor owing to a lower charge-state. These methodologies can be applied to the structural analyses of glycoconjugates with much larger molecular masses and higher polarity, such as the poly-sulfated analogs.
Ii
T
T
Frontier Research Program, Institute of Physical and Chemical Research (RIKEN), Saitama.
Ohashi
Y
Y
Nunomura
S
S
Ogawa
T
T
Nagai
Y
Y
D002240:Carbohydrate Sequence; D013058:Mass Spectrometry / Q000379:methods; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000032:analysis; D000080506:Sialyl Lewis X Antigen; D016339:Spectrometry, Mass, Fast Atom Bombardment; D013431:Sulfates / Q000032:analysis; D014312:Trisaccharides / Q000032:analysis*
D009844:Oligosaccharides; D000080506:Sialyl Lewis X Antigen; D013431:Sulfates; D014312:Trisaccharides