Influence of benzylisothiocyanate and 13-cis-retinoic acid on micronucleus formation induced by benzo[a]pyrene.
The purpose of this study was to investigate the influence of benzylisothiocyanate (BIT) and 13-cis-retinoic acid (RA) upon the genotoxic potential of benzo[a]pyrene (BaP) to induce micronucleus formation in the bone marrow of mice. Eighty-two male mice were divided into 10 groups. One group served as a negative control (olive oil intubation). Four groups received an oral intubation of various concentrations of BIT (15 to 120 mg/kg) and i.p. injections of BaP (185 mg/kg). Another four groups were treated identically, but received RA (20 to 150 mg/kg) in place of BIT. Finally, one group received only i.p. injection of BaP (185 mg/kg). The results showed that both BIT and RA significantly reduced the frequency of micronucleus formation in the bone marrow of the BaP treated animals. BIT was found to be effective at all the tested concentrations. RA was effective only at three of the four tested concentrations (40, 75 and 150 mg/kg). These findings indicate that both BIT and RA may reduce the genotoxic effects of benzo[a]pyrene in the mice under the test conditions utilized.
AlDosari
A
A
Department of Oral Biology, Indiana University, School of Dentistry, Indianapolis 46202, USA.
McDonald
J
J
Olson
B
B
Noblitt
T
T
Li
Y
Y
Stookey
G
G
D000818:Animals; D016587:Antimutagenic Agents / Q000494:pharmacology*; D001564:Benzo(a)pyrene / Q000633:toxicity*; D001853:Bone Marrow; D017879:Isothiocyanates / Q000494:pharmacology*; D015474:Isotretinoin / Q000494:pharmacology*; D008297:Male; D051379:Mice; D015162:Micronucleus Tests; D009153:Mutagens / Q000633:toxicity*
D016587:Antimutagenic Agents; D017879:Isothiocyanates; D009153:Mutagens; D001564:Benzo(a)pyrene; C031403:benzyl isothiocyanate; D015474:Isotretinoin