Isolation of a full-length cDNA clone for human GTP cyclohydrolase I type 1 from pheochromocytoma.
Although the existence of three different cDNA forms of human GTP cyclohydrolase I (GCH I) have been reported (Togari et al., 1992), the full-length sequence of any human GCH I cDNA involving poly (A) tail has not yet been documented. In the present study, we first isolated a full-length cDNA clone encoding human GCH I type 1 from human pheochromocytoma cDNA library. The length of the cDNA insert was 2,921 base pairs including poly (A) tail. RNA blot analysis showed a single mRNA species of 4.0 kb in human pheochromocytoma tissue.
Nomura
T
T
Department of Pharmacology, Fujita Health University School of Medicine, Aichi, Japan.
Ohtsuki
M
M
Matsui
S
S
Sumi-Ichinose
C
C
Nomura
H
H
Hagino
Y
Y
Iwase
K
K
Ichinose
H
H
Fujita
K
K
Nagatsu
T
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000302:isolation & purification*; D004273:DNA, Neoplasm / Q000302:isolation & purification*; D006136:GTP Cyclohydrolase / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D010673:Pheochromocytoma / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics
D018076:DNA, Complementary; D004273:DNA, Neoplasm; D006136:GTP Cyclohydrolase