Parenteral strobilar development of Echinococcus multilocularis in scid mice.
Parenteral strobilation of Echinococcus multilocularis was observed in acid (severe combined immuno-deficient) mice after intracerebral, subcutaneous and intraperitoneal inoculation with protoscoleces. Evaginated protoscoleces and segmented worms were obtained at the inoculated sites. Most worms recovered from peritoneal cavity of scid mice were encapsulated by connective tissue and granulocytes but showed a maximum of 3 proglottids, elongation of genital primordia and vesiculation. Viability of worms recovered from the subcutaneous tissue and peritoneal cavity of scid mice were higher (69.1-91.4%) than those from the immunologically normal C.B-17 (4.0-48.0%) control mice. However, viabilities of worms from the cerebrum of both scid and C.B-17 were almost the same (87.7-94.4%). Worms recovered from scid mice showed further development of reproductive organs when transplanted into the small intestine of prednisolone treated golden hamster. These findings suggest that the parenteral milieu of scid mice allows adult development of E. multilocularis protoscoleces.
Inohara
J
J
Department of Animal Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
Playford
M C
MC
Nonaka
N
N
Oor
H K
HK
Oku
Y
Y
Ito
M
M
Kamiya
M
M
D000818:Animals; D001921:Brain / Q000469:parasitology; D003238:Connective Tissue / Q000469:parasitology; D006224:Cricetinae; D004443:Echinococcosis / Q000469:parasitology / Q000662:veterinary*; D004446:Echinococcus / Q000254:growth & development*; D005260:Female; D008647:Mesocricetus; D051379:Mice; D016513:Mice, SCID / Q000469:parasitology*; D010529:Peritoneal Cavity / Q000469:parasitology; D012376:Rodent Diseases / Q000469:parasitology*