Isolation and characterization of a cDNA from Trichoderma harzianum P1 encoding a 14-3-3 protein homolog.
A full-length cDNA close, Th1433, (GenBank accession No. U24158), was isolated and characterized from the filamentous fungus, Trichoderma harzianum. The deduced amino acid (aa) sequence showed an acidic 30-kDa protein homologous to the 14-3-3 proteins, a family of putative kinase regulators originally characterized in mammalian brain tissue. The greatest homology, 71% identical aa, was found to BMH1, the corresponding protein from Saccharomyces cerevisiae and to the epsilon isoform from sheep brain. Southern analysis of genomic DNA indicated that Th1433 is a member of a small genomic family. At least two genes encoding 14-3-3-like proteins exist in T. harzianum. Northern analysis showed the highest level of expression during the first day after inoculation of the culture with conidial spores.
Klemsdal
S S
SS
Norwegian Crop Research Institute, Department of Plant Pathology, As, Norway. sonja.klemsdal@planteforsk.nlh.no
Hayes
C K
CK
Hjeljord
L
L
Lorito
M
M
Harman
G E
GE
Tronsmo
A
A
D048948:14-3-3 Proteins; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D004271:DNA, Fungal / Q000032:analysis / Q000235:genetics; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics*; D005800:Genes, Fungal / Q000235:genetics; D008969:Molecular Sequence Data; D011506:Proteins / Q000737:chemistry / Q000235:genetics*; D012331:RNA, Fungal / Q000096:biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014242:Trichoderma / Q000235:genetics*; D014446:Tyrosine 3-Monooxygenase
D048948:14-3-3 Proteins; D018076:DNA, Complementary; D004271:DNA, Fungal; D005656:Fungal Proteins; D011506:Proteins; D012331:RNA, Fungal; D012333:RNA, Messenger; C099989:TH1433 protein, Trichoderma harzianum; D014446:Tyrosine 3-Monooxygenase