title: Human papillomavirus infection and transitional cell carcinoma of the bladder. Immunohistochemistry and in situ hybridization.
abstract: The aim of the present research was to investigate human papillomavirus (HPV) infection by means of immunohistochemistry and in situ hybridization in 76 bladder cancer specimens. A biotinylated DNA probe that recognizes HPV 6/11, HPV 16/18 and HPV 31/33/35 was used for in situ hybridization. A polyclonal antibody recognizing HPV capsid antigen (HPVcAg) was used for immunohistochemistry. In situ hybridization and immunohistochemistry were developed by alkaline phosphatase and immunogold-silver techniques respectively. Our results showed that 25 (32.8%) out of 76 bladder carcinoma specimens reacted with HPVcAg. Twelve (15.7%) out of 76 cases were positive for HPV 16/18-DNA using non-isotopic in situ hybridization. Sixteen cases had koilocytosis. No positive signals were found for HPV 6/11 or 31/33/35-DNA probes.
authors.affiliation: Pathology, Unit, Córdoba University Medical School, Spain.
authors.forename: A
authors.initials: A
authors.lastname: López-Beltrán
authors.forename: A L
authors.initials: AL
authors.lastname: Escudero
authors.forename: J C
authors.initials: JC
authors.lastname: Carrasco-Aznar
authors.forename: L
authors.initials: L
authors.lastname: Vicioso-Recio
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D001710:Biotin; D002213:Capsid / Q000276:immunology; D002295:Carcinoma, Transitional Cell / Q000821:virology*; D015344:DNA Probes, HPV; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization / Q000379:methods; D008297:Male; D008875:Middle Aged; D027383:Papillomaviridae / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000175:diagnosis*; D014412:Tumor Virus Infections / Q000175:diagnosis*; D001749:Urinary Bladder Neoplasms / Q000821:virology*
chemical_list: D015344:DNA Probes, HPV; D001710:Biotin