Endothelial-cell injury of the basilar artery caused by ethanol infusion in dogs.
This study in mongrel dogs was designed to observe the effects of ethanol on both endothelial cells of the basilar artery and brain function. By use of sterile surgical technique, a super-selective catheter was placed in the proximal portion of the basilar artery in the dogs. Five dogs received 3 ml of 25% ethanol and 5 dogs received 3 ml of 50% of ethanol through the catheter over 2 minutes. The remaining 5 dogs received 3 ml of saline as a control. Auditory brain stem response (ABR) was monitored for 2 hours after ethanol infusion, and then perfusion-fixation was performed from the heart with 4% paraformaldehyde. The basilar artery was observed with scanning electron microscopy after routine procedures. The endothelial cells were intact in the control group. The 50% group showed a higher level of injury to the endothelium as well as a higher degree of platelet adhesion and fibrin clot formation compared with the 25% group. The extensive endothelial-cell damage subsequently caused thrombus formation. The ABR disappeared immediately after ethanol infusion in both ethanol groups, and recovered gradually in the 25% group, but did not re-appear during the time course of 2 hours in the 50% group. The ethanol less than 25% in concentration near the endothelium is considered to be safe as a transcatheter embolic agent with the attention to the central toxicity.
Kobayashi
H
H
Department of Neurosurgery, Fukui Medical School, Japan.
Ide
H
H
Kabuto
M
M
Handa
Y
Y
Kubota
T
T
D000818:Animals; D001488:Basilar Artery / Q000187:drug effects* / Q000473:pathology; D001933:Brain Stem / Q000098:blood supply / Q000187:drug effects / Q000473:pathology; D004285:Dogs; D004305:Dose-Response Relationship, Drug; D004621:Embolization, Therapeutic; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000473:pathology; D000431:Ethanol / Q000633:toxicity*; D016057:Evoked Potentials, Auditory, Brain Stem / Q000187:drug effects* / Q000502:physiology; D005260:Female; D005337:Fibrin / Q000378:metabolism; D007261:Infusions, Intra-Arterial; D008297:Male; D008855:Microscopy, Electron, Scanning; D010973:Platelet Adhesiveness / Q000187:drug effects
D000431:Ethanol; D005337:Fibrin