title: Immunostaining of p53 protein in ovarian carcinoma: correlation with histopathological data and clinical outcome.
abstract: OBJECTIVE
  The objective of this study was to analyze the incidence of immunohistochemically detectable p53 protein accumulation in epithelial ovarian carcinomas and to correlate these data with the clinical outcome so as to clarify further the role of p53 mutations in prognosis with these patients.
  
  
  METHODS
  Tumor tissues from 179 patients with epithelial ovarian carcinoma were used for immuno-histochemical analysis with monoclonal antibody DO1 and BP 53-12-1 on formalin-fixed, paraffin-embedded tissue.
  
  
  RESULTS
  A total of 78 cases (44%) showed positive nuclear p53 staining. The p53-positive cases were found in all histological types of epithelial ovarian tumors. p53 staining was found in tumors of all stages with a higher percentage of positive cases in stage IV ovarian carcinomas (not significant). Poorly differentiated carcinomas showed a significantly higher percentage of p53 protein expression than did highly differentiated tumors (P = 0.0002). Clinical follow-up of up to 14 years (median 25 months) showed a slightly but not significantly shortened disease-free and overall survival time for patients with p53-positive epithelial ovarian carcinomas.
  
  
  CONCLUSIONS
  We conclude from our data that p53 expression in ovarian carcinoma is associated with poor differentiation but not with the disease being in an advanced stage. There was a tendency for shortened disease-free and overall survival for patients with p53-positive tumors.
authors.affiliation: Department of Gynecology and Obstetrics, Virchow-Klinikum, Humboldt-University, Berlin, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Reles
authors.forename: A
authors.initials: A
authors.lastname: Schmider
authors.forename: M F
authors.initials: MF
authors.lastname: Press
authors.forename: I
authors.initials: I
authors.lastname: Schönborn
authors.forename: W
authors.initials: W
authors.lastname: Friedmann
authors.forename: S
authors.initials: S
authors.lastname: Huber-Schumacher
authors.forename: T
authors.initials: T
authors.lastname: Strohmeyer
authors.forename: W
authors.initials: W
authors.lastname: Lichtenegger
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D009367:Neoplasm Staging; D010051:Ovarian Neoplasms / Q000737:chemistry* / Q000473:pathology / Q000601:surgery; D016159:Tumor Suppressor Protein p53 / Q000032:analysis* / Q000276:immunology
chemical_list: D016159:Tumor Suppressor Protein p53