Reconstitution of GTP-gamma-S-dependent phospholipase D activity with ARF, RhoA, and a soluble 36-kDa protein.
For activation of kidney membrane phospholipase D (PLD), cytosol is absolutely needed in addition to GTP-gamma-S. The active component of cytosol consists of three protein factors: ADP-ribosylation factor, RhoA, and a soluble 36-kDa protein. Any combination of these two factors synergistically activates PLD to some extent, but the presence of the three factors causes full activation. The 36-kDa protein is stable at 60 degrees C but inactivated at 80 degrees C for 10 min. Tissue distribution of the 36-kDa protein roughly coincides with that of PLD, suggesting physiological relevance of the protein in the regulation of PLD.
Shimooku
K
K
Department of Biochemistry, Kobe Univeristy School of Medicine, Kobe, Japan.
Akisue
T
T
Jinnai
H
H
Hitomi
T
T
Ogino
C
C
Yoshida
K
K
Nakamura
S
S
Nishizuka
Y
Y
D020727:ADP-Ribosylation Factors; D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D003600:Cytosol / Q000378:metabolism; D004789:Enzyme Activation; D019204:GTP-Binding Proteins / Q000378:metabolism*; D016244:Guanosine 5'-O-(3-Thiotriphosphate) / Q000378:metabolism*; D008969:Molecular Sequence Data; D010739:Phospholipase D / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism; D012995:Solubility; D020742:rhoA GTP-Binding Protein
D011506:Proteins; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D010739:Phospholipase D; D019204:GTP-Binding Proteins; D020727:ADP-Ribosylation Factors; D020742:rhoA GTP-Binding Protein