Repair of the in vitro HIV-1-induced immunosuppression and blockade of the generation of functional suppressive CD8 cells by anti-alpha interferon and anti-Tat antibodies.
The acute human immunodeficiency virus type 1 (HIV-1) infection of activated peripheral blood mononuclear cells (PBMCs) from normal donors results in inhibition of cell proliferation and generation of functional suppressive T cells. Cultured HIV-1 infected PBMCs but not uninfected PBMCs, following irradiation, can inhibit the proliferation of antigen-activated autologous T cells in a dose-dependent way. CD8+ cell subpopulation is responsible for this inhibition. The presence of anti-alpha interferon (IFN alpha) and anti-Tat antibodies in the culture medium counteracts the HIV-1-induced immunosuppression and prevents the generation of suppressive T cells by these PBMCs. The reported data should have major implications for strategies of AIDS treatment which, in association with antiviral drugs, aim at targetting immune disorders.
Lachgar
A
A
Université Pierre et Marie Curie, Paris, France.
Bernard
J
J
Bizzini
B
B
Astgen
A
A
Le Coq
H
H
Fouchard
M
M
Chams
V
V
Feldman
M
M
Burny
A
A
Zagury
J F
JF
D000163:Acquired Immunodeficiency Syndrome / Q000276:immunology* / Q000821:virology; D000906:Antibodies / Q000494:pharmacology; D018414:CD8-Positive T-Lymphocytes / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects; D015696:Gene Products, tat / Q000276:immunology*; D015497:HIV-1; D006801:Humans; D007108:Immune Tolerance / Q000187:drug effects; D066298:In Vitro Techniques; D016898:Interferon-alpha / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000187:drug effects; D050378:T-Lymphocytes, Regulatory / Q000187:drug effects; D054322:tat Gene Products, Human Immunodeficiency Virus
D000906:Antibodies; D015696:Gene Products, tat; D016898:Interferon-alpha; D054322:tat Gene Products, Human Immunodeficiency Virus