title: Distribution of cellular repopulation and collagen synthesis in a canine anterior cruciate ligament autograft.
abstract: Whether the central core of an anterior cruciate ligament autograft reconstruction is nutritionally compromised at a time when revascularization is known to be complete has not been determined by methods that detect matrix synthesis. In a canine model of anterior cruciate ligament reconstruction with patellar tendon autograft, the adequacy of the supply of metabolites for cellular matrix synthesis was determined by autoradiographic analysis. Total collagen synthesis and cellularity were also quantified. Total collagen synthesis was found to be significantly elevated (p = 0.014 by analysis of variance) in the ligament reconstructions as compared with normal anterior cruciate ligaments or patellar tendons but cellularity was not (p = 0.13 by analysis of variance). Autoradiography demonstrated even distribution of [3H]proline incorporation throughout the graft and normal tissue. When revascularization was complete there was an adequate supply of metabolites for cellular synthesis of protein macromolecules within all regions of the ligament reconstruction. At 3 months after reconstruction, the grafts were found to be actively remodeling their collagen matrix. Since the long-term function of an anterior cruciate ligament autograft is dependent on viable fibroblasts to maintain the collagen matrix the canine anterior cruciate ligament reconstruction contains living cells that are able to remodel the matrix under appropriate conditions.
authors.affiliation: Departments of Orthopaedic Surgery, Cleveland Clinic Foundation, USA.
authors.forename: K P
authors.initials: KP
authors.lastname: Spindler
authors.forename: J T
authors.initials: JT
authors.lastname: Andrish
authors.forename: R R
authors.initials: RR
authors.lastname: Miller
authors.forename: K
authors.initials: K
authors.lastname: Tsujimoto
authors.forename: D I
authors.initials: DI
authors.lastname: Diz
mesh_terms: D000818:Animals; D016118:Anterior Cruciate Ligament / Q000166:cytology / Q000601:surgery*; D001345:Autoradiography; D002455:Cell Division / Q000502:physiology; D003094:Collagen / Q000096:biosynthesis*; D004285:Dogs; D010329:Patella; D013710:Tendons / Q000737:chemistry / Q000637:transplantation*; D014182:Transplantation, Autologous
chemical_list: D003094:Collagen