The nucleus of the human lens: demonstration of a highly characteristic protein pattern by two-dimensional electrophoresis and introduction of a new method of lens dissection.
A practical method for dissection of human lenses is described. The method utilizes the suture patterns as a guide to identify the developmental stage in which fiber cells were formed. Lenses were separated into cortex and adult, infantile, fetal and embryonic nuclear regions. Analysis of the proteins in each of these regions in adult lenses shows that the lens nucleus has a highly characteristic two-dimensional protein pattern distinct from that of the cortex. Each of the nuclear regions has essentially the same protein pattern. The data suggest that the conversion of cortical fibers to mature nuclear fibers involves well controlled processes.
Garland
D L
DL
Laboratory of Mechanisms of Ocular Disease, National Eye Institute, Bethesda, MD 20892, USA.
Duglas-Tabor
Y
Y
Jimenez-Asensio
J
J
Datiles
M B
MB
Magno
B
B
D000293:Adolescent; D000328:Adult; D000886:Anthropometry; D002648:Child; D002675:Child, Preschool; D003459:Crystallins / Q000032:analysis*; D004210:Dissection; D015180:Electrophoresis, Gel, Two-Dimensional; D006652:Histological Techniques; D006801:Humans; D007223:Infant; D007904:Lens Cortex, Crystalline / Q000033:anatomy & histology / Q000737:chemistry; D007907:Lens Nucleus, Crystalline / Q000033:anatomy & histology* / Q000737:chemistry; D008875:Middle Aged
D003459:Crystallins