title: Determination of serotonin released from coffee wax by liquid chromatography.
abstract: A simple hydrolysis and extraction method was developed for the release of serotonin (5-hydroxytryptamine) from a coffee wax sample obtained from decaffeination of coffee beans. The recoverable amount of serotonin was determined by reversed-phase high-performance liquid chromatography with gradient elution and UV detection, using the standard addition method. Different type of basic deactivated chromatographic columns were used for the separation.
authors.affiliation: Department of Medical Chemistry, University Medical School, Pécs, Hungary.
authors.forename: M
authors.initials: M
authors.lastname: Kele
authors.forename: R
authors.initials: R
authors.lastname: Ohmacht
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003069:Coffee / Q000737:chemistry*; D006868:Hydrolysis; D019800:Phenol; D010636:Phenols / Q000302:isolation & purification; D011725:Pyridines / Q000302:isolation & purification; D012701:Serotonin / Q000032:analysis*; D014885:Waxes
chemical_list: D003069:Coffee; D010636:Phenols; D011725:Pyridines; D014885:Waxes; D012701:Serotonin; D019800:Phenol; C023666:pyridine