A recombinant antibody with the antigen-specific, major histocompatibility complex-restricted specificity of T cells.
Specific recognition of peptide/major histocompatibility complex (MHC) molecule complexes by the T-cell receptor is a key reaction in the specific immune response. Antibodies against peptide/MHC complexes would therefore be valuable tools in studying MHC function and T-cell recognition and might lead to novel approaches in immunotherapy. However, it has proven difficult to generate antibodies with the specificity of T cells by conventional hybridoma techniques. Here we report that the phage display technology is a feasible alternative to generate antibodies recognizing specific, predetermined peptide/MHC complexes.
Andersen
P S
PS
Department of Biological Sciences, The Royal Danish School of Pharmacy, Copenhagen, Denmark.
Stryhn
A
A
Hansen
B E
BE
Fugger
L
L
Engberg
J
J
Buus
S
S
D000595:Amino Acid Sequence; D000818:Animals; D000918:Antibody Specificity; D001435:Bacteriophages / Q000235:genetics; D015723:Gene Library; D008285:Major Histocompatibility Complex; D051379:Mice; D008806:Mice, Inbred AKR; D008969:Molecular Sequence Data; D010455:Peptides / Q000276:immunology*; D011994:Recombinant Proteins / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
D010455:Peptides; D011994:Recombinant Proteins