[A simultaneous determination of evodiamine and rutaecarpine in oriental pharmaceutical decoctions containing Evodia fruit by ion-pair high-performance liquid chromatography].
A simple and precise method was established for the simultaneous determination of evodiamine and rutaecarpine in oriental pharmaceutical decoctions containing Evodia Fruit using high-performance liquid chromatography with sodium dodecyl sulfate (SDS) as an ion-pair reagent. Evodiamine and rutaecarpine were eluted within 60 min without interference from co-existing components using an ODS column and a mixture of water-methanol-acetonitrile-SDS-phosphoric acid (600:330:70:5:0.01, v/v/v/w/v, pH 5.0) as a mobile phase.
Ueda
J
J
Tohoku College of Pharmacy, Sendai, Miyagi, Japan.
Momma
N
N
Yamauchi
Y
Y
Yoshie
F
F
Ohsawa
K
K
D000470:Alkaloids / Q000032:analysis*; D002316:Cardiotonic Agents / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D026121:Indole Alkaloids; D010936:Plant Extracts; D011799:Quinazolines / Q000032:analysis*; D052999:Quinazolinones
D000470:Alkaloids; D002316:Cardiotonic Agents; D004365:Drugs, Chinese Herbal; D026121:Indole Alkaloids; D010936:Plant Extracts; D011799:Quinazolines; C028632:rutecarpine; D052999:Quinazolinones; C049639:evodiamine