Carbohydrate-mediated sorting in aggregating embryonal carcinoma cells.
The oligosaccharide structure carried by embryoglycan, Lex hapten, as well as E-cadherin, has been described to mediate Ca(2+)-dependent cell-cell adhesions in embryonal carcinoma cells. To examine the contribution of these two systems to intercellular adhesion, we analyzed aggregation properties of previously isolated embryoglycan-defective mutants of P19 embryonal carcinoma cells. Our data indicate that the absence of Lex and embryoglycan has no effect on homotypic cell aggregation. Pretreatment of the cells with E-cadherin-specific antibody reduced homotypic aggregation of both parental and mutant cells, suggesting that E-cadherin plays a major role in this system. When parental cells were mixed with mutant cells, the aggregates contained either parental or mutant cells; no heterotypic aggregation was observed. The absence of mixed aggregates formed between parental and Lex-or embryoglycan-negative mutant P19 cells suggests that carbohydrates are involved in cadherin-mediated cell sorting.
Boubelík
M
M
Institute of Molecular Genetics, Academy of Sciences of the Czech Republic. boubelik@biomed.cas.cz
Dráberová
L
L
Dráber
P
P
D000818:Animals; D000911:Antibodies, Monoclonal; D015820:Cadherins / Q000737:chemistry / Q000276:immunology / Q000502:physiology*; D002118:Calcium / Q000494:pharmacology; D002240:Carbohydrate Sequence; D018236:Carcinoma, Embryonal; D002448:Cell Adhesion / Q000187:drug effects; D002449:Cell Aggregation / Q000187:drug effects; D002478:Cells, Cultured; D002999:Clone Cells; D004492:Edetic Acid / Q000494:pharmacology; D005434:Flow Cytometry; D007075:Immunoglobulin M; D007700:Kinetics; D016256:Lewis X Antigen / Q000737:chemistry / Q000276:immunology / Q000502:physiology*; D051379:Mice; D008809:Mice, Inbred C3H; D008969:Molecular Sequence Data; D016296:Mutagenesis; D011134:Polysaccharides / Q000172:deficiency / Q000276:immunology / Q000502:physiology*; D014312:Trisaccharides
D000911:Antibodies, Monoclonal; D015820:Cadherins; D007075:Immunoglobulin M; D016256:Lewis X Antigen; D011134:Polysaccharides; D014312:Trisaccharides; C045474:embryoglycan; D004492:Edetic Acid; D002118:Calcium