title: Effect of growth factors on growth of bovine parathyroid cells in serum-free medium.
abstract: Published work has suggested a possible role of the epidermal growth factor receptor (EGFr) in parathyroid disease. Bovine parathyroid cells (BPCs) are frequently used as a tissue model for studying parathyroid disorders. We have studied the effect of the EGFr ligands EGF and transforming growth factor alpha (TGFalpha), alone and with insulin-like growth factors IGF-I and IGF-II on BPC growth. Experiments were run in triplicate and repeated three times. Cell numbers were assessed on day 5 by colorimetric MTT assay as well as by tritiated thymidine uptake. Results show that TGFalpha alone (p < 0.05) and IGF-I and IGF-II alone (p < 0.05) significantly stimulated growth over controls (t-test). Furthermore, the combination of TGFalpha with IGF-I and IGF-II exhibited significant enhancement above that seen with IGF-I and IGF-II alone (p < 0.01). EGF did not stimulate growth over controls. EGFr may be expressed in BPCs, but TGFalpha exhibits a more potent growth stimulus than EGF. Addition of IGF-I or IGF-II to the growth medium further enhances this effect.
authors.affiliation: Department of Surgery, University Hospital of Wales, Heath Park, CF4 4XN Cardiff, U.K.
authors.forename: G P
authors.initials: GP
authors.lastname: Sadler
authors.forename: D L
authors.initials: DL
authors.lastname: Jones
authors.forename: J S
authors.initials: JS
authors.lastname: Woodhead
authors.forename: K
authors.initials: K
authors.lastname: Horgan
authors.forename: M H
authors.initials: MH
authors.lastname: Wheeler
mesh_terms: D000818:Animals; D002417:Cattle; D002452:Cell Count / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003124:Colorimetry; D004396:Coloring Agents; D016895:Culture Media, Serum-Free; D004338:Drug Combinations; D004357:Drug Synergism; D004815:Epidermal Growth Factor / Q000494:pharmacology; D066246:ErbB Receptors / Q000187:drug effects / Q000502:physiology; D006133:Growth Substances / Q000494:pharmacology*; D007334:Insulin-Like Growth Factor I / Q000008:administration & dosage / Q000494:pharmacology; D007335:Insulin-Like Growth Factor II / Q000008:administration & dosage / Q000494:pharmacology; D008854:Microscopy, Electron; D010280:Parathyroid Glands / Q000166:cytology / Q000187:drug effects*; D013778:Tetrazolium Salts; D013844:Thiazoles; D013936:Thymidine / Q000378:metabolism; D016211:Transforming Growth Factor alpha / Q000008:administration & dosage / Q000494:pharmacology; D014316:Tritium
chemical_list: D004396:Coloring Agents; D016895:Culture Media, Serum-Free; D004338:Drug Combinations; D006133:Growth Substances; D013778:Tetrazolium Salts; D013844:Thiazoles; D016211:Transforming Growth Factor alpha; D014316:Tritium; D004815:Epidermal Growth Factor; D007334:Insulin-Like Growth Factor I; D007335:Insulin-Like Growth Factor II; D066246:ErbB Receptors; C022616:thiazolyl blue; D013936:Thymidine