Depressed unloaded sarcomere shortening velocity in acute murine coxsackievirus myocarditis: myocardial remodelling in the absence of necrosis or hypertrophy.
We used right ventricular papillary muscles to study cellular dysfunction in acute murine coxsackievirus myocarditis. We measured unloaded sarcomere shortening velocity (V0) with laser diffraction (HeNe, lambda = 623.8 nm) 7 days after coxsackievirus infection (M) (n = 7) and after infection + monoclonal antibodies to eliminate T cells (T) (n = 4) and in normals (N) (n = 8). A servomotor rapidly shortened a muscle until slack early in contraction and V0 was measured at the onset of zero force. V0 in N was 4.14 +/- 0.84 microns.s-1 at Sl = 2.08 +/- 0.09 microns, 1.70 +/- 0.33 microns.s-1 at 2.06 +/- 0.08 microns in M and, in preliminary experiments, 4.75 +/- 0.96 microns.s-1 at 2.06 +/- 0.07 microns in T. Resting force and stiffness were normal in M. Ventriculor weights in M and T were the same as N. There was an increase in mononuclear cells in M papillary muscles, but no fibrosis or necrosis. Thus, V0 was markedly reduced in acute viral myocarditis in the absence of tissue disruption or hypertrophy, but not if T cells were absent. Pyrophosphate gel electrophoresis showed a shift from predominantly fast in N to a slow myosin isoform in M. Myosin remodelling and reduced unloaded sarcomere shortening velocity occur early in acute coxsackievirus myocarditis and are dependent on immune responses to the virus, but are not a result of histopathological changes.
Hamrell
B B
BB
Department of Molecular Physiology, College of Medicine, University of Vermont, Burlington 05446-2500, USA.
Huber
S A
SA
Leslie
K O
KO
D000818:Animals; D002312:Cardiomyopathy, Hypertrophic / Q000276:immunology / Q000473:pathology / Q000503:physiopathology; D003384:Coxsackievirus Infections / Q000276:immunology / Q000473:pathology* / Q000503:physiopathology; D029822:Enterovirus B, Human / Q000276:immunology / Q000472:pathogenicity; D006352:Heart Ventricles / Q000276:immunology / Q000473:pathology / Q000503:physiopathology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009200:Myocardial Contraction / Q000276:immunology / Q000502:physiology*; D009205:Myocarditis / Q000276:immunology / Q000473:pathology* / Q000503:physiopathology; D009336:Necrosis; D010210:Papillary Muscles / Q000276:immunology / Q000473:pathology / Q000503:physiopathology; D012518:Sarcomeres / Q000276:immunology / Q000473:pathology* / Q000502:physiology; D013601:T-Lymphocytes / Q000276:immunology; D014774:Virulence / Q000276:immunology