Immunological crossreactivity to the catabolite control protein CcpA Bacillus megaterium is found in many gram-positive bacteria.
The catabolite control protein CcpA from Bacillus megaterium was overproduced as a fusion protein to a 6xhis affinity tag and purified to homogeneity. Polyclonal antibodies of high affinity and specificity were raised against the purified protein. The serum did not crossreact with purified Lac repressor despite the fact that CcpA and LacI belong to the same protein family. Using this antiserum we identified proteins that share antigenic determinants with CcpA in many Gram-positive bacteria, including bacilli, staphylococci, lactic acid bacteria, and some actinomycetes.
Küster
E
E
Lehrstuhl für Mikrobiologie, Friedrich-Alexander-Universität Erlagen-Nürnberg, Germany.
Luesink
E J
EJ
de Vos
W M
WM
Hillen
W
W
D000595:Amino Acid Sequence; D000918:Antibody Specificity; D001410:Bacillus megaterium / Q000737:chemistry* / Q000235:genetics / Q000276:immunology*; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology; D015153:Blotting, Western; D003001:Cloning, Molecular; D003429:Cross Reactions; D004268:DNA-Binding Proteins / Q000276:immunology*; D006094:Gram-Positive Bacteria / Q000737:chemistry / Q000276:immunology; D008969:Molecular Sequence Data; D012097:Repressor Proteins / Q000276:immunology*; D012680:Sensitivity and Specificity
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D012097:Repressor Proteins; C069286:catabolite control proteins, bacteria