Identification of bldA mutants of Streptomyces griseus.
The bldA gene (encoding tRNA(UUA)Leu) from Streptomyces griseus (Sg) was cloned by hybridization with bldA from Streptomyces coelicolor (Sc). Introduction of Sg bldA into Sc bldA mutants restored sporulation and actinorhodin production. Sporulation of a subset of Sg bald mutants, which produce no aerial mycelium or spores, was restored in the presence of bldA from Sc or Sg. The nucleotide sequences of the bldA alleles from two such bald mutants revealed point mutations in the anticodon stem and the T psi C stem.
Kwak
J
J
Department of Microbiology, Ohio State University, Columbus 43210, USA.
McCue
L A
LA
Kendrick
K E
KE
D000880:Anthraquinones / Q000378:metabolism; D001483:Base Sequence; D003001:Cloning, Molecular; D017124:Conserved Sequence / Q000235:genetics; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D017354:Point Mutation / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics; D012356:RNA, Transfer, Leu / Q000737:chemistry / Q000235:genetics*; D013171:Spores, Bacterial / Q000235:genetics; D013302:Streptomyces / Q000235:genetics; D013305:Streptomyces griseus / Q000235:genetics*; D013489:Suppression, Genetic; D000095622:Benzoisochromanequinones
D000880:Anthraquinones; D012356:RNA, Transfer, Leu; C013390:actinorhodin; D000095622:Benzoisochromanequinones