Induction of gap junctional intercellular communication by vitamin D in human skin fibroblasts is dependent on the nuclear Induction of gap junctional intercellular communication by vitamin D in human skin fibroblasts is dependent on the nuclear vitamin D receptor.
The physiologically active metabolite of vitamin D, 1alpha,25-dihydroxyvitamin D3 (calcitriol), induces gap junctional intercellular communication in human skin fibroblasts 161BR at a concentration of 10(-7) M. In human skin fibroblasts, FIB5, devoid of a functional nuclear vitamin D receptor (VDR), there is no effect on gap junctional intercellular communication. Parallel to the increase in cell-cell communication, we observed a VDR-dependent increase in connexin43 protein and connexin43 mRNA levels. These results suggest that 1alpha,25-dihydroxyvitamin D3 affects gap junctional intercellular communication at the level of transcription or of mRNA stability via the nuclear VDR.
Clairmont
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A
Institut Für Physiologische Chemie I, Heinrich-Heine-Universitt, Düsseldorf,Germany.
Tessman
D
D
Stock
A
A
Nicolai
S
S
Stahl
W
W
Sies
H
H
D002117:Calcitriol / Q000378:metabolism / Q000494:pharmacology; D002450:Cell Communication / Q000187:drug effects*; D002467:Cell Nucleus / Q000378:metabolism / Q000502:physiology; D018031:Connexin 43 / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D017629:Gap Junctions / Q000187:drug effects*; D006801:Humans; D012333:RNA, Messenger / Q000378:metabolism; D018167:Receptors, Calcitriol / Q000502:physiology*; D012867:Skin / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D014807:Vitamin D / Q000378:metabolism / Q000494:pharmacology*
D018031:Connexin 43; D012333:RNA, Messenger; D018167:Receptors, Calcitriol; D014807:Vitamin D; D002117:Calcitriol