Alpha-fetoprotein binding and uptake by primary cultures of human skeletal muscle.
alpha-Fetoprotein (AFP), a serum alpha-globulin mainly synthesized by the fetal liver and the yolk sac, is the major carrier of polyunsaturated fatty acids during embryo-fetal development. One property characteristic of fetal cells undergoing growth and differentiation is their ability to bind and internalize AFP. In the present work, we have studied the binding and endocytosis of AFP by human muscular cells developing in vitro. Primary cultures of human skeletal muscle, obtained from biopsies and examined at two stages of differentiation (myoblasts and myotubes), were incubated for different times, at 0 and 37 degrees C, with a colloidal-gold-conjugated human AFP probe and studied by light and electron microscopy, as well as by laser scanning confocal microscopy in the reflection mode. The results obtained show that (a) human myoblasts in primary culture bind and internalize the protein, probably through specific AFP receptors, (b) this property is strongly reduced or lost in well-differentiated myotubes, and (c) AFP is also bound, throughout culture development, to the extracellular matrix of fusing myoblasts and differentiated myotubes. The physiological significance of AFP uptake by human myoblasts undergoing growth and differentiation may be based on the ability of AFP to carry and deliver fatty acids to fetal cells.
Lorenzo
H C
HC
Laboratoire de Chimie des Protéines, Institut de Recherches sur le Cancer, Villejuif, France.
Geuskens
M
M
Macho
A
A
Lachkar
S
S
Verdiere-Sahuque
M
M
Pineiro
A
A
Uriel
J
J
D001665:Binding Sites; D018929:Cell Culture Techniques; D003593:Cytoplasm / Q000648:ultrastructure; D005109:Extracellular Matrix / Q000378:metabolism / Q000648:ultrastructure; D006801:Humans; D018613:Microscopy, Confocal; D008854:Microscopy, Electron; D018482:Muscle, Skeletal / Q000166:cytology / Q000378:metabolism*; D011485:Protein Binding; D018000:Receptors, Peptide / Q000378:metabolism* / Q000648:ultrastructure; D000509:alpha-Fetoproteins / Q000378:metabolism*
D018000:Receptors, Peptide; D000509:alpha-Fetoproteins; C042144:alpha-fetoprotein receptor, human