title: cDNA cloning of thylakoid-bound ascorbate peroxidase in pumpkin and its characterization.
abstract: A cDNA for thylakoid-bound ascorbate peroxidase of pumpkin was cloned and characterized. Thylakoid-bound ascorbate peroxidase had a high similarity to cytosolic ascorbate peroxidases, and the precursor contained a transit peptide to chloroplasts at its amino-terminus and a putative membrane-spanning region at its carboxy-terminus.
authors.affiliation: Department of Cell Biology, National Institute for Basic Biology, Okazaki, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Yamaguchi
authors.forename: M
authors.initials: M
authors.lastname: Hayashi
authors.forename: M
authors.initials: M
authors.lastname: Nishimura
mesh_terms: D000595:Amino Acid Sequence; D060387:Ascorbate Peroxidases; D001483:Base Sequence; D002736:Chloroplasts / Q000201:enzymology; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics*; D005638:Fruit / Q000201:enzymology* / Q000235:genetics*; D008969:Molecular Sequence Data; D010544:Peroxidases / Q000235:genetics* / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D018744:DNA, Plant; D010544:Peroxidases; D060387:Ascorbate Peroxidases