Study of cellulose acetate membrane-based glucose biosensors.
A new immobilization procedure was designed to covalently immobilize enzymes onto a cellulose acetate membrane that was simple and easy to handle. The resultant enzyme membrane was very stable, with high specific activity. A glucose oxidase membrane was prepared by using this method, which was combined either with an oxygen electrode or with a hydrogen peroxide electrode to construct a glucose biosensor. The response characteristics of both biosensors were compared.
Wang
S
S
Shanghai Institute of Biochemistry, Chinese Academy of Sciences, China.
Ji
X
X
Yuan
Z
Z
D015374:Biosensing Techniques; D002482:Cellulose / Q000031:analogs & derivatives*; D004800:Enzymes, Immobilized; D005947:Glucose; D005949:Glucose Oxidase; D006861:Hydrogen Peroxide; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008567:Membranes, Artificial; D015203:Reproducibility of Results; D013696:Temperature; D013997:Time Factors
D004800:Enzymes, Immobilized; D008567:Membranes, Artificial; C005062:acetylcellulose; D002482:Cellulose; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase; D005947:Glucose