Plasticity in expression of immunoreactivity for neuropeptide Y, enkephalins and neurotensin in the hypothalamic tubero-infundibular dopaminergic system during lactation in mice.
In lactating nursing vs lactating pup-deprived mice, single or multiple immunolabeling was performed to compare immunoreactivities (ir) for neuropeptide Y (NPY), enkephalins (ENK) and neurotensin (NT) in the tyrosine hydroxylase (TH)-ir (ENK) and neurotensin (NT) in the tyrosine hydroxylase (TH)-ir hypothalamic tubero-infundibular dopaminergic (TIDA) system. NPY-, ENK- and NT-irs were intensely expressed and coexisted in virtually all TH-ir endings in the median eminence (ME) of nursing mice. Removal of the pups induced a marked depletion of the peptide-irs from the ME TH-ir endings. In the arcuate nucleus (ARC) of colchicine-treated nursing mice which received peripheral injections of Fluoro-Gold (FG) to retrogradely label neuroendocrine cells, virtually all dorsal A12 TH-ir perikarya simultaneously expressed, with individual variations, NPY-, ENK- and NT-irs, and all contained FG. These results suggest that the synthesis of NPY, ENK and NT is enhanced in TIDA neurons during lactation and that these neuromessengers may be co-released together with DA from the ME to regulate the suckling-induced prolactin secretion at the hypothalamic and/or pituitary levels.
Ciofi
P
P
U. 156 INSERM, Lille, France.
Crowley
W R
WR
Pillez
A
A
Schmued
L L
LL
Tramu
G
G
Mazzuca
M
M
D000818:Animals; D001111:Arcuate Nucleus of Hypothalamus / Q000378:metabolism; D003078:Colchicine / Q000494:pharmacology; D004298:Dopamine / Q000502:physiology*; D004745:Enkephalins / Q000096:biosynthesis*; D005260:Female; D005456:Fluorescent Dyes; D007031:Hypothalamus / Q000378:metabolism*; D007150:Immunohistochemistry; D007774:Lactation / Q000378:metabolism*; D051379:Mice; D009473:Neuronal Plasticity / Q000502:physiology; D009478:Neuropeptide Y / Q000096:biosynthesis*; D009496:Neurotensin / Q000096:biosynthesis*; D013266:Stilbamidines; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
C049774:2-hydroxy-4,4'-diamidinostilbene, methanesulfonate salt; D004745:Enkephalins; D005456:Fluorescent Dyes; D009478:Neuropeptide Y; D013266:Stilbamidines; D009496:Neurotensin; D014446:Tyrosine 3-Monooxygenase; D003078:Colchicine; D004298:Dopamine