Demonstration of a glycoprotein derived from the 24p3 gene in mouse uterine luminal fluid.
A glycoprotein in mouse uterine luminal fluid was purified to homogeneity via a series of purification steps involving Sephadex G-100 chromatography, Sephadex G-50 chromatography and HPLC on a reverse-phase C18 column, in that order. Automated Edman degradation was unable to determine the N-terminal residue of the glycoprotein and the partial sequences determined from its trypsin digests were found to be identical with the protein sequence deduced from 24p3 cDNA. The core protein and the total amount of carbohydrate together gave a molecular mass of 25.8 kDa. Results from the characterization of the glycopeptide bond indicated the presence of N-linked carbohydrate but no O-linked carbohydrate in the protein, which has two potential sites for N-linked carbohydrate at Asn81 and Asn85, as deduced from analysis of the primary structure. The core protein was shown to have a molecular mass equal to that of the putative protein deduced from cDNA, suggesting that this protein may contain no signal peptide. Results of Northern-blot analysis for various tissues of adult mice revealed that the 24p3 gene was expressed in lung, spleen, uterus, vagina and epididymis.
Chu
S T
ST
Institute of Biological Chemistry, Academia Sinica, Taipel, Taiwan, Republic of China.
Huang
H L
HL
Chen
J M
JM
Chen
Y H
YH
D000209:Acute-Phase Proteins / Q000737:chemistry* / Q000235:genetics* / Q000302:isolation & purification; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006023:Glycoproteins / Q000737:chemistry* / Q000302:isolation & purification; D006031:Glycosylation; D000071068:Lipocalin-2; D054834:Lipocalins; D051379:Mice; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009005:Monosaccharides / Q000032:analysis; D015513:Oncogene Proteins / Q000737:chemistry* / Q000235:genetics* / Q000302:isolation & purification; D010446:Peptide Fragments / Q000737:chemistry; D016133:Polymerase Chain Reaction; D017385:Sequence Homology; D015843:Serpins; D014357:Trypsin / Q000378:metabolism
D000209:Acute-Phase Proteins; D006023:Glycoproteins; D000071068:Lipocalin-2; D054834:Lipocalins; D009005:Monosaccharides; D015513:Oncogene Proteins; D010446:Peptide Fragments; D015843:Serpins; C029708:uterine luminal fluid proteins; C071332:Lcn2 protein, mouse; D014357:Trypsin