title: Distribution of copper in biopsied canine liver.
abstract: Biopsied canine liver tissues were investigated histochemically by electron microscopy to demonstrate copper localization. The samples were fixed and stained with the modified sulfide-silver method with a combination of iron and zinc removal. Using this method, the hepatic copper, even at a low concentration of 30 micrograms/g dry weight, was detectable. Copper was found in the hepatocyte lysosomes or in the cytoplasm as electrondense granules.
authors.affiliation: Department of Clinical Pathology, Nippon Veterinary and Animal Science University, Tokyo, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Kawamura
authors.forename: T
authors.initials: T
authors.lastname: Washizu
authors.forename: T
authors.initials: T
authors.lastname: Ishida
authors.forename: I
authors.initials: I
authors.lastname: Tomoda
mesh_terms: D000818:Animals; D001706:Biopsy; D003300:Copper / Q000032:analysis*; D003593:Cytoplasm / Q000737:chemistry / Q000648:ultrastructure; D004285:Dogs; D005260:Female; D006651:Histocytochemistry; D008099:Liver / Q000737:chemistry* / Q000648:ultrastructure; D008247:Lysosomes / Q000737:chemistry / Q000648:ultrastructure; D008297:Male; D008854:Microscopy, Electron; D013194:Staining and Labeling / Q000379:methods / Q000662:veterinary; D014018:Tissue Distribution
chemical_list: D003300:Copper