Purification and activity of recombinant chicken stem cell factor produced by using a baculovirus vector.
Stem cell factor is a pleiotropic cytokine that plays an essential role in the development of hematopoietic cells, germ cells, and melanocytes. To obtain recombinant soluble chicken stem cell factor (chSCF), a baculovirus containing the cDNA encoding chSCF polypeptide from amino acids -25 to 170 was constructed. The chSCF produced in insect cells infected with the virus was purified by ion exchange column chromatography. The ability of the purified protein to induce the outgrowth of neurites from chicken dorsal root ganglia cultured in vitro was demonstrated.
Zhou
J H
JH
Institute of Society for Techno-innovation of Agriculture, Forestry and Fisheries, Ibaraki, Japan.
Ohtaki
M
M
Inumaru
S
S
Kitani
H
H
Sakurai
M
M
D000818:Animals; D016367:Baculoviridae / Q000235:genetics*; D001483:Base Sequence; D002460:Cell Line; D002478:Cells, Cultured; D002645:Chickens / Q000235:genetics*; D002852:Chromatography, Ion Exchange / Q000379:methods / Q000662:veterinary; D017931:DNA Primers / Q000737:chemistry; D018076:DNA, Complementary / Q000032:analysis / Q000737:chemistry / Q000235:genetics; D005727:Ganglia, Spinal / Q000166:cytology; D005822:Genetic Vectors; D007313:Insecta; D008969:Molecular Sequence Data; D016501:Neurites / Q000187:drug effects; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology; D019089:Stem Cell Factor / Q000235:genetics* / Q000302:isolation & purification* / Q000494:pharmacology
D017931:DNA Primers; D018076:DNA, Complementary; D011994:Recombinant Proteins; D019089:Stem Cell Factor