Use of the polymerase chain reaction for the detection of reticuloendotheliosis virus in Marek's disease vaccines and chicken tissues.
Reticuloendotheliosis virus (REV) proviral DNA appears to be a frequent contaminant in Marek's disease (MD) vaccines. A polymerase chain reaction (PCR) was established and evaluated for its ability to detect REV proviral DNA in infected cell cultures and chicken tissues. Deoxynucleotide primers were selected from the highly conserved gag region of the REV genome. The amplification products were identified by electrophoresis, nested PCR and by hybridization with a digoxigenine-labelled oligonucleotide. The PCR results correlated well with the diagnosis obtained by conventional procedures, i.e. virus isolation or indirect immuno-fluorescence test (IIFT).
Reimann
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Federal Research Centre for Virus Diseases of Animals, Friedrich-Loeffler-Institutes, Isle of Riems, Germany.
Werner
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O
D000818:Animals; D001483:Base Sequence; D002642:Chick Embryo; D002645:Chickens; D017931:DNA Primers / Q000737:chemistry; D004279:DNA, Viral / Q000032:analysis* / Q000737:chemistry; D019084:Fluorescent Antibody Technique, Indirect / Q000662:veterinary; D016679:Genome, Viral; D008381:Herpesvirus 2, Gallid / Q000276:immunology*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011533:Proviruses / Q000235:genetics* / Q000302:isolation & purification; D017986:Reticuloendotheliosis Viruses, Avian / Q000235:genetics* / Q000302:isolation & purification; D012680:Sensitivity and Specificity; D014765:Viral Vaccines / Q000737:chemistry* / Q000592:standards
D017931:DNA Primers; D004279:DNA, Viral; D014765:Viral Vaccines