[Antioxidative enzyme activity in the blood of healthy persons].
BACKGROUND
Under physiological conditions the dynamic balance between the reactive oxygen production and detoxication is maintained.


OBJECTIVE
The study was aimed at the detection of the activity of antioxidant enzymes: superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) in erythrocytes and the level of malondialdehyde (MDA) in the serum of healthy subjects, their relation to age, body weight, sex, smoking, and the possible correlation between individual parameters.


METHODS
The authors have examined 128 healthy volunteers (57 men and 71 women) from 20 to 72 years of age. The results were statistically evaluated by means of the ANOVA programme.


RESULTS
The group of healthy people had a large dispersion of interindividual activities of antioxidant enzymes and MDA levels. Age and weight were reversely correlated to the CAT activity in erythrocytes. The CAT activity was significantly higher in women than in men, and the GPx activity was lower in smokers than in non-smokers. The rest of investigated parameters were not significantly influenced by sex of examined people and smoking. Mutual correlation between individual enzymes were highly significant.


CONCLUSIONS
A large dispersion of activities of the investigated enzymes and MDA levels in healthy individuals do not allow to make a standard use of these parameters in clinical practice. (Tab. 5, Fig. 2, Ref. 20.).
Volkovová
K
K
Výskumný ústav výzivy v Bratislave, Slovakia.
Beno
I
I
Staruchová
M
M
Bobek
P
P
Mekinová
D
D
Tatara
M
M
D000328:Adult; D000368:Aged; D000975:Antioxidants / Q000378:metabolism; D002374:Catalase / Q000097:blood*; D005260:Female; D005979:Glutathione Peroxidase / Q000097:blood*; D006801:Humans; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D008875:Middle Aged; D012016:Reference Values; D012907:Smoking / Q000097:blood; D013482:Superoxide Dismutase / Q000097:blood*
D000975:Antioxidants; D008315:Malondialdehyde; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase
Aktivita antioxidacných enzýmov v krvi zdrav ch osôb.