Protection against oxidative damage by dihydroflavonols in Engelhardtia chrysolepis.
Dihydroflavonol taxifolin and its glycoside, astilbin, from Engelhardtia chrysolepis were evaluated as antioxidants and radical scavengers. These dihydroflavonols inhibited superoxide anion production in the xanthine/xanthine oxidase system. Microsomal lipid peroxidation induced by NADPH-cytochrome P-450 reductase was also inhibited by these flavonoids. Mitochondrial lipid peroxidation was inhibited only by the aglycon. Taxifolin protected peroxy radical-damaged mitochondria with no effect on enzyme activity. Furthermore, taxifolin and astilbin protected red cells against oxidative hemolysis. These dihydroflavonols were found to be effective for protecting subcellular systems and red blood cells against oxidative stress in vitro.
Haraguchi
H
H
Faculty of Engineering, Fukuyama University, Japan.
Mochida
Y
Y
Sakai
S
S
Masuda
H
H
Tamura
Y
Y
Mizutani
K
K
Tanaka
O
O
Chou
W H
WH
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002417:Cattle; D005419:Flavonoids / Q000494:pharmacology*; D044948:Flavonols; D006461:Hemolysis; D006801:Humans; D015227:Lipid Peroxidation; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism; D018384:Oxidative Stress; D011794:Quercetin / Q000031:analogs & derivatives* / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D013481:Superoxides / Q000378:metabolism; D014197:Trees / Q000378:metabolism
D000975:Antioxidants; D005419:Flavonoids; D044948:Flavonols; D013481:Superoxides; C099069:astilbin; D011794:Quercetin; C003377:taxifolin