In vivo expression of intercellular adhesion molecule 1 in type II pneumocytes during hyperoxia.
Cell-to-cell communication is often disrupted when tissue damage occurs, triggering new signals to cope with the injury. The expression of intercellular adhesion molecule (ICAM-1), a protein involved in the migration, binding, and activation of leukocytes, is markedly increased in mouse lungs damaged by acute hyperoxic exposure. Type I alveolar epithelial cells are sensitive to hyperoxic lung injury, and must be removed from the air spaces following their destruction. In contrast, type II pneumocytes are relatively resistant to hyperoxia and may have a role in the removal process. Two reports demonstrate increased ICAM-1 in alveoli after hyperoxia (Welty et al., 1993, AJRCMB 9:393-400; and Kang et al., 1993, AJRCMB 9:350-355), but the cellular site(s) of ICAM-1 synthesis were not determined. We hypothesized that during in vivo exposure to 100% oxygen (O2), type II pneumocytes synthesize and secrete ICAM-1, an important step in attracting inflammatory cells to the site of injury. Adult mice were exposed to 100% O2 for up to 72 h. To determine whether type II cells express ICAM-1, tissue sections were studied by electron microscopy single-label in situ hybridization or light microscopy dual-label in situ hybridization, using radiolabeled and nonradiolabeled probes. In the lungs of unexposed animals, ICAM-1 mRNA was detected in many cells-including type I pneumocytes-but not in type II cells. After hyperoxia, ICAM-1 transcripts were detected in bona fide, surfactant protein C mRNA-containing, type II alveolar epithelial cells. This observation suggests that type II cells play an important and previously unrecognized role in pulmonary inflammation from O2 toxicity and emphasizes the importance of type II pneumocytes in alveolar repair after injury.
Piedboeuf
B
B
Department of Pediatrics (Neonatology), Centre de Recherche du C.H.U.L., Laval University, Sainte-Foy, Québec, Canada.
Frenette
J
J
Petrov
P
P
Welty
S E
SE
Kazzaz
J A
JA
Horowitz
S
S
D000818:Animals; D004848:Epithelium / Q000473:pathology / Q000502:physiology / Q000648:ultrastructure; D018496:Hyperoxia / Q000473:pathology / Q000503:physiopathology*; D017403:In Situ Hybridization; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics*; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D016253:Microscopy, Immunoelectron; D011650:Pulmonary Alveoli / Q000166:cytology* / Q000648:ultrastructure; D012333:RNA, Messenger / Q000032:analysis
D012333:RNA, Messenger; D018799:Intercellular Adhesion Molecule-1