title: A general assay for restriction endonucleases and other DNA-modifying enzymes with plasmid substrates.
abstract: A procedure for measuring the activities of enzymes that alter the covalent structure of DNA is described. The assay utilizes covalently closed circles of DNA as the substrate and yields quantitative data on the fraction of this DNA converted to both open-circle and linear forms.
authors.affiliation: Department of Biochemistry, Centre for Molecular Recognition, University of Bristol, UK.
authors.forename: I B
authors.initials: IB
authors.lastname: Vipond
authors.forename: G S
authors.initials: GS
authors.lastname: Baldwin
authors.forename: M
authors.initials: M
authors.lastname: Oram
authors.forename: S G
authors.initials: SG
authors.lastname: Erskine
authors.forename: L M
authors.initials: LM
authors.lastname: Wentzell
authors.forename: M D
authors.initials: MD
authors.lastname: Szczelkun
authors.forename: T J
authors.initials: TJ
authors.lastname: Nobbs
authors.forename: S E
authors.initials: SE
authors.lastname: Halford
mesh_terms: D010582:Bacteriophage lambda / Q000235:genetics; D004254:DNA Nucleotidyltransferases / Q000032:analysis / Q000378:metabolism; D004262:DNA Restriction Enzymes / Q000032:analysis* / Q000378:metabolism; D004270:DNA, Circular / Q000737:chemistry; D015246:Deoxyribonuclease EcoRI / Q000502:physiology; D004587:Electrophoresis, Agar Gel; D009691:Nucleic Acid Denaturation; D010957:Plasmids / Q000235:genetics; D011995:Recombination, Genetic; D019895:Transposases
chemical_list: D004270:DNA, Circular; D004254:DNA Nucleotidyltransferases; D019895:Transposases; D004262:DNA Restriction Enzymes; D015246:Deoxyribonuclease EcoRI