title: Copy-dependent and position-independent expression of rat aldolase A gene.
abstract: In order to understand the molecular mechanisms of the temporal and spatial differences of gene expression in higher organisms, rat aldolase A gene carrying two distinct promoters was introduced into fertilized eggs and the resulting transgenic mice were analyzed. The transgene expression is tissue-specific and is developmentally regulated. In addition, the expression is regulated in a copy-dependent manner irrespective of where the transgene is integrated, suggesting that a mechanism excluding the effect of the integration site exists within the transgene itself. To explore the conformational change of this gene in the genome, the DNase I hypersensitive sites of the gene were examined. Three sites (DHS-1,2, and 3) were identified upstream and downstream of the gene and these sites were retained in the transgene as well as in the gene observed endogenously.
authors.affiliation: Department of Bioscience, National Cardiovascular Center Research Institute, Osaka.
authors.forename: K
authors.initials: K
authors.lastname: Hashido
authors.forename: Y
authors.initials: Y
authors.lastname: Arai
authors.forename: S
authors.initials: S
authors.lastname: Kajihara
authors.forename: K
authors.initials: K
authors.lastname: Joh
authors.forename: H
authors.initials: H
authors.lastname: Yatsuki
authors.forename: K
authors.initials: K
authors.lastname: Hori
authors.forename: J
authors.initials: J
authors.lastname: Miyazaki
authors.forename: K
authors.initials: K
authors.lastname: Yamamura
authors.forename: T
authors.initials: T
authors.lastname: Mukai
mesh_terms: D000818:Animals; D001665:Binding Sites; D003850:Deoxyribonuclease I / Q000378:metabolism; D005634:Fructose-Bisphosphate Aldolase / Q000235:genetics*; D015971:Gene Expression Regulation, Enzymologic; D051379:Mice; D008822:Mice, Transgenic; D009690:Nucleic Acid Conformation; D051381:Rats; D012680:Sensitivity and Specificity
chemical_list: D003850:Deoxyribonuclease I; D005634:Fructose-Bisphosphate Aldolase