pMPY-ZAP: a reusable polymerase chain reaction-directed gene disruption cassette for Saccharomyces cerevisiae.
Gene disruption is an important method for genetic analysis in Saccharomyces cerevisiae. We have designed a polymerase chain reaction-directed gene disruption cassette that allows rapid disruption of genes in S. cerevisiae without previously cloning them. In addition, this cassette allows recycling of URA3, generating gene disruptions without the permanent loss of the ura3 marker. An indefinite number of disruptions can therefore be made in the same strain.
Schneider
B L
BL
Cold Spring Harbor Laboratory, NY 11724, USA.
Steiner
B
B
Seufert
W
W
Futcher
A B
AB
D001483:Base Sequence; D005819:Genetic Markers; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional / Q000379:methods*; D009964:Orotidine-5'-Phosphate Decarboxylase / Q000235:genetics; D016133:Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D012641:Selection, Genetic; D014170:Transformation, Genetic
D005819:Genetic Markers; D009964:Orotidine-5'-Phosphate Decarboxylase