Transgenic Arabidopsis tester lines with dominant marker genes.
The map positions of a set of eight T-DNA insertions in the Arabidopsis genome have been determined by using closely linked visible markers. The insertions are dispersed over four of the five chromosomes. Each T-DNA insert contains one or more of the chimeric marker genes neomycin phosphotransferase (neo), hygromycin phosphotransferase (hpt), phosphinothricin acetyltransferase (bar), beta-glucuronidase (gusA) and indole-3-acetamide hydrolase (iaaH). The neo, hpt and bar marker genes are dominant in a selective germination assay or when used as DNA markers in a polymerase chain reaction. These dominant markers will allow recombinants to be discerned in a germinating F2 population, one generation earlier than with a conventional recessive marker. The transgenic marker lines will speed up and simplify the isolation of recombinants in small genetic intervals, a rate-limiting step in positional cloning strategies. The transgenic lines containing the hpt marker will also be of interest for the isolation of deletion mutants at the T-DNA integration sites.
Van Lijsebettens
M
M
Laboratorium voor Genetica, VIB Universiteit Gent, Belgium.
Wang
X
X
Cnops
G
G
Boerjan
W
W
Desnos
T
T
Höfte
H
H
Van Montagu
M
M
D000123:Acetyltransferases / Q000235:genetics; D000581:Amidohydrolases / Q000235:genetics; D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics*; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular / Q000379:methods*; D004251:DNA Transposable Elements / Q000235:genetics*; D005799:Genes, Dominant; D017343:Genes, Plant / Q000235:genetics*; D005819:Genetic Markers / Q000235:genetics; D005966:Glucuronidase / Q000235:genetics; D019868:Kanamycin Kinase; D008969:Molecular Sequence Data; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics; D030821:Plants, Genetically Modified / Q000201:enzymology / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D011995:Recombination, Genetic
D004251:DNA Transposable Elements; D005819:Genetic Markers; D000123:Acetyltransferases; C056243:phosphinothricin N-acetyltransferase; D017853:Phosphotransferases (Alcohol Group Acceptor); C063242:hygromycin-B kinase; D019868:Kanamycin Kinase; D005966:Glucuronidase; D000581:Amidohydrolases; C058772:indole-3-acetamide hydrolase