title: Guanidine-HCl extraction of proteins expressed in Escherichia coli using polycistronic operons based on the pUR expression vectors.
authors.affiliation: Institute of Bioorganic Chemistry, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia.
authors.forename: K V
authors.initials: KV
authors.lastname: Pugachev
authors.forename: A G
authors.initials: AG
authors.lastname: Pletnev
mesh_terms: D000818:Animals; D023181:Antimicrobial Cationic Peptides; D001483:Base Sequence; D004669:Encephalitis Viruses, Tick-Borne / Q000235:genetics*; D004926:Escherichia coli / Q000737:chemistry* / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics*; D019791:Guanidine; D006146:Guanidines; D006801:Humans; D008969:Molecular Sequence Data; D010452:Peptide Biosynthesis; D010455:Peptides / Q000235:genetics / Q000302:isolation & purification; D014176:Protein Biosynthesis; D011489:Protein Denaturation; D011506:Proteins / Q000235:genetics / Q000302:isolation & purification; D020365:RNA Helicases; D011898:Ranidae / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification*; D012259:Ribonuclease, Pancreatic; D012697:Serine Endopeptidases; D012997:Solvents; D017361:Viral Nonstructural Proteins / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification*; D001616:beta-Galactosidase / Q000235:genetics; D043583:Nucleoside-Triphosphatase; D053487:DEAD-box RNA Helicases; D000085242:Viral Proteases
chemical_list: D023181:Antimicrobial Cationic Peptides; D019791:Guanidine; D006146:Guanidines; D010455:Peptides; D011506:Proteins; D020365:RNA Helicases; D011993:Recombinant Fusion Proteins; D012259:Ribonuclease, Pancreatic; D012697:Serine Endopeptidases; D012997:Solvents; D017361:Viral Nonstructural Proteins; D001616:beta-Galactosidase; C060028:NS3 protein, flavivirus; D043583:Nucleoside-Triphosphatase; D053487:DEAD-box RNA Helicases; D000085242:Viral Proteases; C067773:magainin A; C046366:angiogenin