Acetylcholinesterase from Bungarus venom: a monomeric species.
The venom of Bungarus fasciatus, an Elapidae snake, contains a high level of AChE activity. Partial peptide sequences show that it is closely homologous to other AChEs. Bungarus venom AChE is a non-amphiphilic monomeric species, a molecular form of AChE which has not been previously found in significant levels in other tissues. The composition of carbohydrates suggests the presence of N-glycans of the 'complex' and 'hybrid' types. Ion exchange chromatography, isoelectric focusing and electrophoresis in non-denaturing and denaturing conditions reveal a complex microheterogeneity of this enzyme, which is partly related to its glycosylation.
Cousin
X
X
Unité des Venins, Institut Pasteur, Paris, France.
Créminon
C
C
Grassi
J
J
Méflah
K
K
Cornu
G
G
Saliou
B
B
Bon
S
S
Massoulié
J
J
Bon
C
C
D000110:Acetylcholinesterase / Q000032:analysis* / Q000737:chemistry / Q000633:toxicity; D000595:Amino Acid Sequence; D000818:Animals; D002038:Bungarotoxins / Q000737:chemistry* / Q000633:toxicity; D017838:Bungarus; D002241:Carbohydrates / Q000032:analysis; D004563:Electrochemistry; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid
D002038:Bungarotoxins; D002241:Carbohydrates; D000110:Acetylcholinesterase