Expression and purification strategies for the production of single-chain antibody and T-cell receptor fragments in E. coli.
This work describes protocols for the production of single-chain antibody and T-cell receptor fragments in E. coli. A choice of methods is given for the purification of the recombinant fragments that rely on the use of either immunoaffinity or metal chelate affinity chromatography. The TCR fragments may have to be denatured and refolded before the fragments attain their proper conformation.
Molloy
P E
PE
Department of Molecular and Cell Biology, Marischal College, University of Aberdeen, Scotland.
Graham
B M
BM
Cupit
P M
PM
Grant
S D
SD
Porter
A J
AJ
Cunningham
C
C
D002846:Chromatography, Affinity; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000737:chemistry / Q000235:genetics*; D015870:Gene Expression; D005798:Genes, Bacterial; D007128:Immunoglobulin Fragments / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification; D007136:Immunoglobulins / Q000737:chemistry / Q000302:isolation & purification; D010446:Peptide Fragments / Q000737:chemistry / Q000302:isolation & purification; D010957:Plasmids / Q000235:genetics; D017433:Protein Structure, Secondary; D011948:Receptors, Antigen, T-Cell / Q000096:biosynthesis*; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification
D007128:Immunoglobulin Fragments; D007136:Immunoglobulins; D010446:Peptide Fragments; D011948:Receptors, Antigen, T-Cell; D011994:Recombinant Proteins