title: Elevation of acid glycosidase activities in thyroid and gastric tumors.
abstract: Numerous investigators have suggested that cell glycoconjugates are modified by the development of cancer and the progression of this to a malignant form. Accordingly, in the present work, beta-D-galactosidase, alpha-L-fucosidase, beta-N-acetyl-D-glucosaminidase and beta-N-acetyl-D-galactosaminidase activities were studied in human thyroid and gastric tumours. Samples were obtained from human gastric mucosa and thyroid gland tumours together with a part of the surrounding normal tissue (control). Enzyme activity was determined spectrophotometrically based on the release of p-nitrophenol from suitable p-nitrophenyl-derivative substrates. Results showed that beta-D-galactosidase, alpha-L-fucosidase, beta-N-acetyl-D-glucosaminidase and beta-N-acetyl-D-galactosaminidase activities were detected in tumour and control samples from thyroid and gastric tissues. The gastric mucosa also showed alpha-L-mannosidase activity. The specific activities of these glycosidases were higher (two- or three-fold) in tumour tissues as compared with their controls. beta-D-galactosidase, beta-N-acetyl-D-glucosaminidase and beta-N-acetyl-D-galactosaminidase activities from thyroid and gastric tumours showed a significant increase in V(max) as compared with their respective controls (P < 0.05 or P < 0.001). Thyroid alpha-L-fucosidase activity showed a statistically and significantly increased affinity (lower K(m)) in tumour samples as compared to normal tissue. In conclusion both gastric and thyroid tumours showed enhanced glycosidase activity as compared with enzyme activity observed in normal tissue. These results are in agreement with the notion of a markedly raised degradation within lysosomes of tumour cells.
authors.affiliation: Department of Fundamental Biology, Faculty of Sciences, University of Vigo, Spain.
authors.forename: E
authors.initials: E
authors.lastname: Gil-Martín
authors.forename: S
authors.initials: S
authors.lastname: Gil-Seijo
authors.forename: C
authors.initials: C
authors.lastname: Nieto-Novoa
authors.forename: A
authors.initials: A
authors.lastname: Fernández-Briera
mesh_terms: D000118:Acetylglucosaminidase / Q000378:metabolism; D000143:Acids; D000230:Adenocarcinoma / Q000201:enzymology*; D000704:Analysis of Variance; D006026:Glycoside Hydrolases / Q000378:metabolism*; D006596:Hexosaminidases / Q000378:metabolism; D006801:Humans; D013274:Stomach Neoplasms / Q000201:enzymology*; D013964:Thyroid Neoplasms / Q000201:enzymology*; D014020:Tissue Extracts / Q000378:metabolism; D005644:alpha-L-Fucosidase / Q000378:metabolism; D001616:beta-Galactosidase / Q000378:metabolism; D048828:beta-N-Acetyl-Galactosaminidase
chemical_list: D000143:Acids; D014020:Tissue Extracts; D006026:Glycoside Hydrolases; D006596:Hexosaminidases; D001616:beta-Galactosidase; D005644:alpha-L-Fucosidase; D000118:Acetylglucosaminidase; D048828:beta-N-Acetyl-Galactosaminidase