Nod factors produced by Rhizobium leguminosarum biovar viciae induce ethylene-related changes in root cortical cells of Vicia sativa ssp. nigra.
Vicia sativa ssp. nigra plants develop the "Thick short root" (Tsr) phenotype when both (i) the roots are inoculated with the root nodule inducing bacterium Rhizobium leguminosarum biovar viciae, and (ii) the plants, including the roots, are grown in the light. Tsr roots have a reduced length, are locally twice as thick as normal roots and have an increased number of root hairs. Development of the Tsr phenotype is correlated with the presence of nod (nodulation) genes in the rhizobia. Nod factors (lipochitin oligosaccharides), products of these nod genes, can induce the Tsr phenotype in the absence of rhizobia. The Tsr phenotype can be mimicked by addition of the ethylene-releasing compound ethephon. Using several microscopical techniques, we compared roots showing the Tsr phenotype (Tsr roots) with normal roots and roots grown in the presence of the ethylene inhibitor aminoethoxyvinylglycine (AVG). The thickening of Tsr roots appeared to be caused by a swelling of the cortical cells, which corresponded with (i) a reorientation of the interphase cortical microtubules from a transverse to a longitudinal direction, (ii) general cell wall modifications, (iii) frequent absence of middle lamellae, and (iv) local maceration. The same changes could be induced by ethephon and were inhibited by AVG. This strongly suggests that the Tsr phenotype is caused by excessive ethylene production. The ethylene-related changes mentioned above are also seen during infection thread formation, but only very locally. Apparently, Vicia roots when grown in the light overrespond to Nod factors leading to overproduction of ethylene and to a non-local "ripening" process. These phenomena inhibit nodulation of the main root by preventing formation of pre-infected threads and by reducing formation of root nodule primordia. Local controlled production of ethylene, as induced by Nod factors, may, however, be an essential element of the nodulation process.
van Spronsen
P C
PC
Institute of Molecular Plant Sciences, Leiden University, The Netherlands.
van Brussel
A A
AA
Kijne
J W
JW
D002473:Cell Wall / Q000737:chemistry / Q000648:ultrastructure; D005030:Ethylenes / Q000378:metabolism*; D005998:Glycine / Q000031:analogs & derivatives / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D008870:Microtubules / Q000737:chemistry / Q000648:ultrastructure; D009943:Organophosphorus Compounds / Q000494:pharmacology; D010937:Plant Growth Regulators / Q000494:pharmacology; D018517:Plant Roots / Q000737:chemistry / Q000382:microbiology / Q000502:physiology*; D016961:Rhizobium leguminosarum / Q000378:metabolism* / Q000502:physiology; D013559:Symbiosis
D005030:Ethylenes; D008070:Lipopolysaccharides; C092736:Nod factor, Rhizobium leguminosarum; D009943:Organophosphorus Compounds; D010937:Plant Growth Regulators; C088137:aminoethoxyvinylglycine; D005998:Glycine; C005073:ethephon