title: Dynamics of ammonia uptake in nitrogen limited anaerobic cultures of Saccharomyces cerevisiae.
abstract: Dynamics of the ammonia uptake by Saccharomyces cerevisiae under anaerobic conditions was studied in ammonia limited continuous cultures. A large number of pulse additions of ammonia (25-100 mg 1(-1)) were made at different dilution rates (0.05-0.20 h-1). The response was followed by on-line monitoring of the carbon dioxide evolution rate (CER), optical density, and by frequent analysis of extra- and intracellular metabolites. The uptake of a pulse of ammonia proceeded in a qualitatively highly reproducible pattern. Initially, a rapid and growth rate dependent uptake of ammonia was observed (lasting for about 10-15 min). Next followed a phase with little uptake (approx. 5 min). Finally, the rest of the ammonia pulse was taken up at a somewhat smaller rate which also depended on the growth rate. The first phase coincided with an increase in CER caused by mobilization of the intracellular carbohydrate trehalose and subsequently of glycogen. Regardless of dilution rate and the amount of ammonia added, the initial high uptake rate of ammonia was maintained until approximately the same amount of ammonia had been taken up. Transition from the first to the second uptake phase was associated with an increased glycerol production, indicating an elevated anabolic activity.
authors.affiliation: Department of Biotechnology, Technical University of Denmark, Lyngby, Denmark.
authors.forename: U
authors.initials: U
authors.lastname: Schulze
authors.forename: G
authors.initials: G
authors.lastname: Lidén
authors.forename: J
authors.initials: J
authors.lastname: Villadsen
mesh_terms: D000641:Ammonia / Q000378:metabolism*; D000693:Anaerobiosis; D001692:Biological Transport; D018533:Biomass; D003470:Culture Media; D007700:Kinetics; D009584:Nitrogen / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000254:growth & development / Q000378:metabolism*
chemical_list: D003470:Culture Media; D000641:Ammonia; D009584:Nitrogen