Cationic liposomes enhance retrovirus-mediated multinucleated cell formation and retroviral transduction.
A retroviral vector carrying herpes simplex virus thymidine kinase (HSV-TK) gene was introduced into psi 2 packaging cells (psi 2tkn) and XC tumor cells (XCtkn). psi 2tkn, XCtkn and XC cells co-cultured with TK-carrying cells were killed by ganciclovir (GCV) treatment. The growth of XC cells transplanted into nude mice was also suppressed by intratumoral injection of psi 2tkn or XCtkn cells and subsequent GCV administration. In addition, the XC cells cultured with either psi 2tkn cells or cell-free retrovirus suspension formed giant multinucleated cells. The multinucleated cell formation was specific to the combination of XC cells and the retrovirus produced by the psi 2tkn cells. Cationic liposomes enhanced the retrovirus-induced multinucleated cell formation and retroviral transduction. The correlation between the two actions of liposomes suggests that liposomes which enhance multinucleated cell formation are potent enhancers of retroviral transduction.
Kaneko
Y
Y
First Department of Medicine, Faculty of Medicine, University of Tokyo, Japan.
Tsukamoto
A
A
D000818:Animals; D002412:Cations; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D015774:Ganciclovir / Q000008:administration & dosage; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors; D008081:Liposomes / Q000737:chemistry*; D051379:Mice; D012190:Retroviridae; D013937:Thymidine Kinase / Q000235:genetics; D014161:Transduction, Genetic
D002412:Cations; D008081:Liposomes; D013937:Thymidine Kinase; D015774:Ganciclovir