Cervical cytology by means of fluorescence in situ hybridization with a set of chromosome-specific DNA probes.
OBJECTIVE
To reveal the numerical aberration of chromosome 1 and chromosome 17 in cervical neoplasia.


METHODS
Fluorescence in situ hybridization (FISH) with chromosome-specific repetitive DNA probes was applied on cervical smears of cervical intraepithelial neoplasia (CIN) I, CIN II, and CIN III, and of invasive carcinoma cases, to detect numerical aberrations of chromosome 1 (#1) and chromosome 17 (#17). The cases were histologically classified as CIN I (n = 9), CIN II (n = 12), CIN III [severe dysplasia (n = 14), carcinoma in situ (CIS) (n = 14)], and invasive carcinoma (IC) squamous-cell carcinoma, large-cell nonkeratinizing type (n = 12). FISH was applied on the same cervical smears of these cases after Papanicolaou's staining, and copies in marked atypical cells were counted using a fluorescence microscope.


RESULTS
The 9.49 +/- 2.59%/9.72 +/- 1.40% (#1/#17) cells showed an aneuploid pattern in CIN I, 22.5 +/- 3.98%/15.5 +/- 3.02% (#1/#17) in CIN II, 44.3 +/- 7.18%/ 45.01 +/- 5.61% (#1/#17) in severe dysplasia, 52.66 +/- 6.32/48.9 +/- 7.55% (#1/#17) in CIS, and 66.22 +/- 3.20%/57.38 +/- 5.35% in IC. The loss of a chromosome in CIN III cases and the gain of a chromosome in CIS and IC cases should be noted (p < 0.01).


CONCLUSIONS
Because FISH has revealed the numerical aberrations of chromosome 1 and 17 in cervical neoplasia, this is an especially useful method for biological dosimetry and cancer biology.
Hariu
H
H
Department of Obstetrics and Gynecology, Iwate Medical University, Morioka, Japan.
Matsuta
M
M
D002278:Carcinoma in Situ / Q000235:genetics* / Q000473:pathology; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D002584:Cervix Uteri / Q000473:pathology; D002869:Chromosome Aberrations / Q000235:genetics*; D002878:Chromosomes, Human, Pair 1 / Q000235:genetics*; D002886:Chromosomes, Human, Pair 17 / Q000235:genetics*; D015342:DNA Probes; D004273:DNA, Neoplasm / Q000032:analysis; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D012189:Retrospective Studies; D002578:Uterine Cervical Dysplasia / Q000235:genetics* / Q000473:pathology; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000473:pathology; D014626:Vaginal Smears
D015342:DNA Probes; D004273:DNA, Neoplasm