title: The binding site of the IclR repressor protein overlaps the promoter of aceBAK.
abstract: In Escherichia coli, repression of the aceBAK operon is mediated by the IclR protein. We used an in vitro oligonucleotide selection technique to determine the consensus recognition sequence for MR. Mutational analysis confirmed the contribution of this sequence to repression in vivo and identified the -35 element of the promoter.
authors.affiliation: Department of Biochemistry, University of Minnesota, Minneapolis, Minnesota 55455, USA.
authors.forename: B
authors.initials: B
authors.lastname: Pan
authors.forename: I
authors.initials: I
authors.lastname: Unnikrishnan
authors.forename: D C
authors.initials: DC
authors.lastname: LaPorte
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D001483:Base Sequence; D001665:Binding Sites; D016384:Consensus Sequence; D004269:DNA, Bacterial / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins; D007522:Isocitrate Lyase / Q000235:genetics; D008292:Malate Synthase / Q000235:genetics; D008969:Molecular Sequence Data; D016296:Mutagenesis; D009876:Operon; D010749:Phosphoprotein Phosphatases / Q000235:genetics; D011401:Promoter Regions, Genetic; D017346:Protein Serine-Threonine Kinases / Q000235:genetics; D012097:Repressor Proteins / Q000378:metabolism*; D014157:Transcription Factors
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; D012097:Repressor Proteins; D014157:Transcription Factors; C067479:IclR protein, E coli; D008292:Malate Synthase; D017346:Protein Serine-Threonine Kinases; C042301:isocitrate dehydrogenase kinase; C042300:isocitrate dehydrogenase phosphatase; D010749:Phosphoprotein Phosphatases; D007522:Isocitrate Lyase