Relationship between stress response toward bile salts, acid and heat treatment in Enterococcus faecalis.
Stress tolerance and cross-protection in Enterococcus faecalis ATCC19433 were examined after exposure to bile salts, acid or heat shock. Bile salts and heat adapted cells demonstrated induced homologous tolerance and cross-resistance. No cross-protection of heat adapted cells against acid stress is observed and pretreatment with bile salts even sensitized the cells to this challenge. Whole-cell protein extract analysis revealed that each treatment induced a battery of stress proteins. Some of these polypeptides are induced by more than one treatment. The greatest overlap is observed between bile salts and heat treatments. Eighteen stress proteins, including DnaK and GroEL, are common between these stresses.
Flahaut
S
S
Laboratoire de Microbiologie de l'Environnement, Université de Caen, France. lme@unicaen.fr
Hartke
A
A
Giard
J C
JC
Benachour
A
A
Boutibonnes
P
P
Auffray
Y
Y
D001647:Bile Acids and Salts / Q000494:pharmacology; D018834:Chaperonin 60 / Q000096:biosynthesis; D013293:Enterococcus faecalis / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D029968:Escherichia coli Proteins; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis; D006360:Heat-Shock Proteins / Q000096:biosynthesis* / Q000302:isolation & purification; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration
D001647:Bile Acids and Salts; D018834:Chaperonin 60; D029968:Escherichia coli Proteins; D018840:HSP70 Heat-Shock Proteins; D006360:Heat-Shock Proteins; C063842:dnaK protein, E coli