title: The polyubiquitin gene of the mosquito Anopheles gambiae: structure and expression.
abstract: The polyubiquitin gene from the mosquito Anopheles gambiae has been cloned and sequenced, and its structure is reported along with sequence analysis results. The gene consists of approximately seven tandem head-to-tail repeat units of the seventy-six amino acid-coding ubiquitin monomer. It is expressed constitutively in larvae, pupae and adults of An. gambiae, as well as in a cell line derived from this mosquito species. A probe made from a DNA fragment containing the coding region of the gene recognizes transcripts of approximately 3.6 kb and 4.4 kb in RNA isolated from all mosquito developmental stages and a unique transcript of approximately 3.0 kb in RNA from the cell line. Single monomeric units of the An. gambiae polyubiquitin gene shared from 75.9% to 85.5% identity at the DNA level with homologous sequences from other organisms ranging from yeast to man. A comparison of individual repeat units of the An. gambiae gene revealed that, in general, the 5' ends of the individual monomers are more highly conserved than the 3' ends. The gene mapped by in situ hybridization on ovarian nurse cell polytene chromosomes to a primary site at division 12C on chromosome 2R and to a secondary site at division 9C on the same chromosome.
authors.affiliation: Division of Parasitic Diseases, Centers for Disease Control and Prevention, US Public Health Service, Atlanta, GA 30341-3724, USA.
authors.forename: C B
authors.initials: CB
authors.lastname: Beard
authors.forename: A J
authors.initials: AJ
authors.lastname: Cornel
authors.forename: F H
authors.initials: FH
authors.lastname: Collins
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000852:Anopheles / Q000235:genetics*; D001483:Base Sequence; D001704:Biopolymers / Q000235:genetics*; D002874:Chromosome Mapping; D005260:Female; D015870:Gene Expression; D017344:Genes, Insect; D008297:Male; D008969:Molecular Sequence Data; D025821:Polyubiquitin; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D014452:Ubiquitins / Q000235:genetics*
chemical_list: D001704:Biopolymers; D014452:Ubiquitins; D025821:Polyubiquitin