Repetitive element sequence-based PCR for species and strain discrimination in the genus Listeria.
Repetitive element sequence-based PCR (rep-PCR) was used to generate DNA fingerprints for Listeria spp. Two primer sets (REP 1R-I REP 2-I and ERIC 1R ERIC 2) used in respectively REP- and ERIC-PCR revealed that bacteria of the genus Listeria possess short repetitive extragenic palindromic elements and enterobacterial repetitive intergenic consensus sequences. Specific band profiles obtained by ERIC-PCR enabled the identification of Listeria species. With both REP- and ERIC-PCR the L. monocytogenes serotypes 1/2a, 1/2b, 1/2c, 3b and 4b could be clearly distinguished from each other. Within the serotype 1/2a, REP-PCR showed a higher discriminative potential than ERIC-PCR and a comparable discriminative potential as RAPD combining 3-4 primers.
Jersek
B
B
Department of Food Science and Technology, University of Ljubijana, Biotechnical Faculty, Slovenia. barbara.jersek@uni-lj.si
Tcherneva
E
E
Rijpens
N
N
Herman
L
L
D001483:Base Sequence; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000235:genetics*; D008087:Listeria / Q000145:classification* / Q000235:genetics; D008089:Listeria monocytogenes / Q000145:classification / Q000235:genetics; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*; D013045:Species Specificity
D004269:DNA, Bacterial