A recombinant PCR approach requiring only three non-chimeric primers to generate a minigene of interest.
The recombinant PCR allows construction of chimeric molecules. Here we describe this approach utilizing non-chimeric primers. Unlike previous recombinant PCR methods, this approach eliminates the need of multiple sets of primers and multiple rounds of PCR making it an economical and expeditious alternative. We have used this approach to generate an FGF-1 minigene.
Chotani
M A
MA
Program in Molecular, Cellular and Developmental Biology, Ohio State University, Columbus 43210, USA.
Chiu
I M
IM
D001483:Base Sequence; D017931:DNA Primers; D005346:Fibroblast Growth Factors / Q000235:genetics; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*
D017931:DNA Primers; D005346:Fibroblast Growth Factors