Quantitation of human cytomegalovirus (HCMV) DNA in cerebrospinal fluid by competitive PCR in AIDS patients with different HCMV central nervous system diseases.
The current study was designed to quantitate human cytomegalovirus (HCMV) DNA in cerebrospinal fluid (CSF) of persons with AIDS with specific HCMV-related CNS disease. DNA present in CSF obtained from AIDS patients was initially detected by a qualitative PCR procedure and then quantitated using a competitive PCR assay. In a group of 21 AIDS patients with HCMV-related CNS disease, 12 patients with HCMV polyradiculopathy had a mean +/- SEM of 11,982 +/- 4,480 copies/microliters in their CSF compared to 1,747 +/- 929 for 9 patients with HCMV encephalitis p = 0.017). Of the 14 patients with > 1,000 copies/microliters of HCMV DNA in CSF, 11(79%) had HCMV polyradiculopathy including all 3 with > 10,000 copies/microliters. Ganciclovir treatment of 3 patients with HCMV-related CNS disease was associated with a decline in HCMV DNA detectable within CSF. These data indicate that quantities of HCMV DNA in CSF are higher in persons with HCMV-related polyradiculopathy than encephalitis, and that quantitation of HCMV DNA can be useful in monitoring antiviral therapy.
Shinkai
M
M
Department of Pediatrics, University of California, San Diego, La Jolla, USA.
Spector
S A
SA
D017088:AIDS-Related Opportunistic Infections / Q000134:cerebrospinal fluid* / Q000188:drug therapy / Q000821:virology; D000998:Antiviral Agents / Q000627:therapeutic use; D002493:Central Nervous System Diseases / Q000134:cerebrospinal fluid* / Q000821:virology; D003587:Cytomegalovirus / Q000302:isolation & purification*; D003586:Cytomegalovirus Infections / Q000134:cerebrospinal fluid* / Q000188:drug therapy; D004279:DNA, Viral / Q000032:analysis / Q000134:cerebrospinal fluid*; D018792:Encephalitis, Viral / Q000134:cerebrospinal fluid / Q000821:virology; D015774:Ganciclovir / Q000627:therapeutic use; D006801:Humans; D016133:Polymerase Chain Reaction; D011128:Polyradiculopathy / Q000134:cerebrospinal fluid / Q000821:virology; D011379:Prognosis
D000998:Antiviral Agents; D004279:DNA, Viral; D015774:Ganciclovir