title: Rapid, small scale purification of rice hoja blanca and Echinochloa hoja blanca tenuivirus ribonucleoprotein.
abstract: Highly purified tenuivirus ribonucleoprotein was obtained from small amounts of leaf tissue by sedimenting the ribonucleoprotein particles from debris-free plant extract into a 30% sucrose cushion, in 1.5-mL microfuge tubes. Using this protocol, significant size differences were discovered in the double-stranded forms of the viral genomic RNAs of rice hoja blanca tenuivirus and a tenuivirus isolated from Echinochloa colonum, a common weed of rice cultivation.
authors.affiliation: CIBCM, University of Costa Rica, San José, Costa Rica.
authors.forename: J R
authors.initials: JR
authors.lastname: de Miranda
authors.forename: A M
authors.initials: AM
authors.lastname: Espinoza
authors.forename: R
authors.initials: R
authors.lastname: Hull
mesh_terms: D012275:Oryza / Q000821:virology; D010942:Plant Viruses / Q000737:chemistry*; D010944:Plants / Q000821:virology; D012328:RNA Viruses / Q000737:chemistry*; D012261:Ribonucleoproteins / Q000302:isolation & purification*; D014764:Viral Proteins / Q000302:isolation & purification*
chemical_list: D012261:Ribonucleoproteins; D014764:Viral Proteins