Cloning of extracellular lipase gene from Xanthomonas campestris pathovar sesami on to Escherichia coli.
A lipase gene from X. campestris pv. sesami (strain XcS 1) causal agent of leaf spot disease of Sesamum indicum, was cloned onto E. colt. XcS showed the presence of lip+ transformants on Dye's medium with 1% glycerol as sole carbon source. The recombinant plasmids were isolated and when digested with Eco R1, yielded 2 fragments with molecular weights 4.0 and 4.8 kb. Thus a 8.8 kb insert DNA fragment was obtained which showed lipase activity.
Sheela
P
P
Centre for Advanced Study in Botany, University of Madras.
Amuthan
G
G
Mahadevan
A
A
D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D008049:Lipase / Q000235:genetics*; D016959:Xanthomonas campestris / Q000201:enzymology / Q000235:genetics*
D008049:Lipase