Universality of mitochondrial RNA editing in cytochrome-c oxidase subunit I (coxI) among the land plants.
Plant mitochondrial pre-mRNAs often undergo C-to-U conversions, a phenomenon termed RNA editing. The molecular source of specificity and phylogenetic depth of the editing machinery remain to be determined. We amplified coxI gene fragments via the polymerase chain reaction from a diversity of taxa within the land plants, and sequenced each. Alignment and comparison of 25 homologous coxI gene sequences with those from plant species having known RNA editing sites which restore amino acid sequence consensus was used to infer sites of C-to-U conversions. Our results, derived using the comparative approach, imply that the plant mitochondrial editing machinery extends throughout vascular plant phylogeny, and also that this phenomenon is present in every major branch of the (non-vascular) Bryophyta: liverworts (Hepaticae), hornworts (Anthocerotae), and mosses (Musci). These results have important consequences for our thoughts on the evolutionary history of the plant RNA editing process, as they imply that editing is older than was previously believed.
Sper-Whitis
G L
GL
Department of Zoology, Miami University, Oxford, OH 45056, USA.
Moody
J L
JL
Vaughn
J C
JC
D000595:Amino Acid Sequence; D001483:Base Sequence; D003576:Electron Transport Complex IV / Q000235:genetics*; D007438:Introns; D008969:Molecular Sequence Data; D010802:Phylogeny; D010944:Plants / Q000235:genetics*; D012313:RNA / Q000235:genetics*; D017393:RNA Editing; D000077278:RNA, Mitochondrial
D000077278:RNA, Mitochondrial; D012313:RNA; D003576:Electron Transport Complex IV