title: Genetic diversity in marine algal virus communities as revealed by sequence analysis of DNA polymerase genes.
abstract: Algal-virus-specific PCR primers were used to amplify DNA polymerase gene (pol) fragments (683 to 689 bp) from the virus-sized fraction (0.02 to 0.2 microns) concentrated from inshore and offshore water samples collected from the Gulf of Mexico. Algal-virus-like DNA pol genes were detected in five samples collected from the surface and deep chlorophyll maximum. PCR products from an offshore station were cloned, and the genetic diversity of 33 fragments was examined by restriction fragment length polymorphism and sequence analysis. The five different genotypes or operational taxonomic units (OTUs) that were identified on the basis of restriction fragment length polymorphism banding patterns were present in different relative abundances (9 to 34%). One clone from each OTU was sequenced, and phylogenetic analysis showed that all of the OTUs fell within the family Phycodnaviridae. Four of the OTUs fell within a group of viruses (MpV) which infect the photosynthetic picoplankter Micromonas pusilla. The genetic diversity among these genotypes was as large as that previously found for MpV isolates from different oceans. The remaining genotype formed its own clade between viruses which infect M. pusilla and Chrysochromulina brevifilum. These results imply that marine virus communities contain a diverse assemblage of MpV-like viruses, as well as other unknown members of the Phycodnaviridae.
authors.affiliation: Department of Marine Science, University of Texas at Austin, Port Aransas 78373-1267, USA. fchen2@uga.cc.uga.edu
authors.forename: F
authors.initials: F
authors.lastname: Chen
authors.forename: C A
authors.initials: CA
authors.lastname: Suttle
authors.forename: S M
authors.initials: SM
authors.lastname: Short
mesh_terms: D001483:Base Sequence; D004259:DNA-Directed DNA Polymerase / Q000235:genetics*; D056890:Eukaryota / Q000821:virology*; D014644:Genetic Variation; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D014780:Viruses / Q000235:genetics*; D014871:Water Microbiology
chemical_list: D004259:DNA-Directed DNA Polymerase