Centripetal transport of herpes simplex virus in human retinal pigment epithelial cells in vitro.
Herpes simplex virus displays tropism for neurons and other polarized epithelial cells. We have grown human retinal pigment epithelial cells in culture to study potential mechanisms whereby herpes simplex virus (type I) is transported from the plasma membrane of the cell to the nucleus. The cells were highly polarized as determined by a variety of criteria. They were tightly coupled by junctional complexes, as determined by electron microscopy, immunofluorescent staining of tight junctions and measurements of transepithelial electrical resistances > 200 omega cm2. Immunofluorescence and confocal microscopy were used to visualize microtubule orientation. The microtubules were arranged (i) in a single apical cilium, (ii) in a meshwork beneath the apical membrane and (iii) in longitudinally arranged bundles near the lateral membranes and nucleus. The latter microtubules were primarily oriented with their plus ends directed toward the basal surface of the cells. We infected retinal pigment epithelial cells at the apical surface with virus and assayed the uptake and transport of virus to the nucleus by quantitative immunoblot and immunocytochemical staining for the viral immediate early gene product, infected cell protein 4. The antigen first appeared in retinal pigment epithelial cells 2 h after infection. Treatment of the cells with 33 microM nocodazole, a microtubule-destabilizing drug, delayed the appearance of the viral antigen by 1 h. The effect of nocodazole treatment on microtubule integrity was confirmed by immunofluorescent staining and immunoblots of tubulin. Both cytoplasmic dynein and the ubiquitous form of kinesin were identified in the cells using immunoblots. These novel data indicate that human retinal pigment epithelial cells, like neurons, are susceptible to infection by herpes simplex virus and that the centripetal transport of virus to the nucleus in both cell types is facilitated by microtubules. The orientation of microtubules in retinal pigment epithelial cells suggests that the transport of herpes simplex virus from the apical surface is mediated by a microtubule-activated motor enzyme, possibly kinesin.
Topp
K S
KS
Department of Anatomy, University of California San Francisco 94143, USA.
Bisla
K
K
Saks
N D
ND
Lavail
J H
JH
D001692:Biological Transport / Q000502:physiology; D015153:Blotting, Western; D016764:Cell Polarity / Q000502:physiology; D002478:Cells, Cultured / Q000378:metabolism / Q000821:virology; D004398:Dyneins / Q000502:physiology; D006801:Humans; D016547:Kinesins / Q000502:physiology; D018613:Microscopy, Confocal; D008854:Microscopy, Electron; D008870:Microtubules / Q000737:chemistry / Q000187:drug effects; D015739:Nocodazole / Q000494:pharmacology; D010857:Pigment Epithelium of Eye / Q000378:metabolism* / Q000648:ultrastructure / Q000821:virology*; D018139:Simplexvirus / Q000378:metabolism* / Q000648:ultrastructure
D004398:Dyneins; D016547:Kinesins; D015739:Nocodazole