title: In vitro attachment of endothelial cells to different graft materials.
abstract: The purpose of this study was to compare the degree of endothelial cell attachment to different synthetic vascular graft materials. Human microvessel endothelial cells were labelled with indium-111-oxine, injected into 9 different vascular grafts and left to adhere for 40 min. Unattached cells were removed and radioactivity of the grafts measured. Grafts were flushed with 20 ml phosphate-buffered saline during 5 s to remove cells with poor attachment and radioactivity was measured again. Attachment differed between the grafts. In order of decreasing attachment the grafts were albumin-coated Dacron (47%), preclotted Dacron (35-44%), gelatin-coated Dacron (20%), gelatin-coated polyurethane (17%), collagen-coated Dacron (12%), preclotted expanded polytetrafluoroethylene (ePTFE; 3%). Albumin-coated Dacron showed the highest degree of cell attachment of ePTFE the lowest. Statistically significant differences exist between ePTFE and all other grafts and between albumin-coated and collagen-coated Dacron, gelatin-coated polyurethane and gelatin-coated Dacron.
authors.affiliation: Department of Surgery, Lund University, Malmö, Sweden.
authors.forename: N
authors.initials: N
authors.lastname: Jensen
authors.forename: B
authors.initials: B
authors.lastname: Lindblad
authors.forename: D
authors.initials: D
authors.lastname: Bergqvist
mesh_terms: D001807:Blood Vessel Prosthesis; D002448:Cell Adhesion; D004730:Endothelium, Vascular / Q000166:cytology*; D006801:Humans; D066298:In Vitro Techniques