Mating-induced FOS-like immunoreactivity in the rat forebrain: a sex comparison and a dimorphic effect of pelvic nerve transection.
Previous research has shown that mating induces the expression of the immediate-early gene, c-fos, as detected by the increased presence of nuclear FOS-like immunoreactivity (FOS-IR), in specific forebrain regions of both male and female rats. In the male both olfactory-vomeronasal (O/V) and genital/somatosensory (G/S) inputs appear to contribute to the neural FOS response to mating whereas in the female G/S input carried by the pelvic nerves appears to mediate the forebrain FOS response. To date, however, no direct sex comparison of the mating-induced forebrain FOS response has been made in rats maintained under the same steroidal conditions nor has the contribution of afferent sensory input from the pelvic nerves been assessed in males. We first compared the level of FOS-IR in brain regions of mated and unpaired gonadectomized male and female rats given 5 micrograms/kg estradiol benzoate (EB) for 7 days and 500 micrograms progesterone (P) 4 h prior to testing. One h after experiencing 1 ejaculation, both sexes showed increased FOS-IR in the medial preoptic a (MPOA), bed nucleus of the stria terminalis (BNST), medial amygdala, the ventro-lateral portion of the ventromedial nucleus of the hypothalamus (VMN), and the midbrain central tegmental field (CTF). This increase was significantly greater in the MPOA and medial amygdala of mated females than of males. Bilateral transection of the pelvic nerves significantly attenuated the increase in FOS-IR after mating in the CTF of male rats and in the MPOA, BNST, VMN, medial amygdala and CTF of females. Thus, following mating there is no sex difference in the brain regions which express c-fos, but there is a dimorphism in the contribution of afferent information conveyed by the pelvic nerves to the mating-induced FOS response. The neural FOS response of the female to mating is heavily dependent upon the G/S afferent inputs carried by the pelvic nerves whereas the male's neural c-fos response may depend on O/V input plus G/S input conveyed via other efferent pathways such as the pudendal nerves.
Wersinger
S R
SR
Department of Biology, Boston University, MA 02215, USA.
Baum
M J
MJ
Erskine
M S
MS
D000818:Animals; D004542:Ejaculation / Q000502:physiology; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D007150:Immunohistochemistry; D008297:Male; D009919:Orchiectomy; D010052:Ovariectomy; D010525:Peripheral Nerves / Q000502:physiology*; D011301:Preoptic Area / Q000166:cytology / Q000502:physiology; D016548:Prosencephalon / Q000033:anatomy & histology / Q000378:metabolism*; D016760:Proto-Oncogene Proteins c-fos / Q000096:biosynthesis*; D051381:Rats; D012727:Sex Characteristics; D012726:Sexual Behavior, Animal / Q000502:physiology*; D013787:Thalamic Nuclei / Q000502:physiology; D014697:Ventromedial Hypothalamic Nucleus / Q000166:cytology / Q000378:metabolism
D016760:Proto-Oncogene Proteins c-fos; D004958:Estradiol