title: [Applications of luminescence reactions in life science].
abstract: Bioluminescence and chemiluminescence have emerged in the last decade as a major tool for biochemical and biological studies. Several very sensitive assays have been developed in our laboratory such as enzymatic assays, immunoassays and detection of nucleic acids. The use of a new instrumentation allowing to dimensional photon counting has permit the emergence of new investigations at the cellular level. In addition to the advantages of sensitivity and the real-time non-invasive nature of this detection system, the imaging potential of using low-light and photon counting video cameras has been particularly influential in establishing its ascendence over more traditional systems. This review provides a reflection in this field through several applications in life sciences.
authors.affiliation: Centre de Recherches INSERM, Montpellier, France.
authors.forename: J C
authors.initials: JC
authors.lastname: Nicolas
authors.forename: A M
authors.initials: AM
authors.lastname: Boussioux
authors.forename: A
authors.initials: A
authors.lastname: Joyeux
authors.forename: P
authors.initials: P
authors.lastname: Balaguer
authors.forename: B
authors.initials: B
authors.lastname: Térouanne
authors.forename: S
authors.initials: S
authors.lastname: Roux
authors.forename: D
authors.initials: D
authors.lastname: Gagne
authors.forename: M
authors.initials: M
authors.lastname: Pons
mesh_terms: D000255:Adenosine Triphosphate / Q000032:analysis; D007124:Immunoenzyme Techniques; D066298:In Vitro Techniques; D008163:Luminescent Measurements; D009249:NADP / Q000032:analysis; D015341:Nucleic Acid Probes; D017382:Reactive Oxygen Species / Q000032:analysis
chemical_list: D015341:Nucleic Acid Probes; D017382:Reactive Oxygen Species; D009249:NADP; D000255:Adenosine Triphosphate
vernacular_title: Applications des réactions de luminescence dans le domaine des sciences de la vie.