Immunohistochemical detection of rinderpest virus: effects of autolysis and period of fixation.
Samples of eyelid, tongue, soft palate and palatine tonsil were collected from calves infected experimentally with rinderpest virus. The tissues were fixed in 10 per cent neutral buffered formalin immediately, 24 or 48 hours post mortem. Then, after three days, 10 days, 28 days or three months in formalin, they were processed into paraffin blocks and examined immunohistochemically for rinderpest viral antigen. The tonsil was the best of the four tissues in providing a consistently positive immunohistochemical signal for the presence of virus, despite autolytic changes and/or prolonged fixation.
Brown
C C
CC
Foreign Animal Disease Diagnostic Laboratory, NVSL-VS-APHIS-USDA, Plum Island, New York, USA.
Ojok
L
L
Mariner
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JC
D000818:Animals; D001329:Autolysis; D002417:Cattle; D005143:Eyelids / Q000473:pathology / Q000821:virology; D005557:Formaldehyde; D006652:Histological Techniques; D007150:Immunohistochemistry / Q000379:methods; D010160:Palate, Soft / Q000473:pathology / Q000821:virology; D014066:Palatine Tonsil / Q000473:pathology / Q000821:virology; D011180:Postmortem Changes; D012301:Rinderpest / Q000175:diagnosis* / Q000473:pathology / Q000821:virology; D012302:Rinderpest virus / Q000302:isolation & purification*; D013997:Time Factors; D014059:Tongue / Q000473:pathology / Q000821:virology
D005557:Formaldehyde