Irsogladine inhibits ionomycin-induced decrease in intercellular communication in cultured rabbit gastric epithelial cells.
Effects of irsogladine on ionomycin-induced decrease in intercellular communication and increase in intracellular concentration of Ca2+ ([Ca2+]i) were investigated in cultured rabbit gastric epithelial cells. Ionomycin (10(-7)-10(-6) M) transiently and concentration-dependently inhibited intercellular communication concomitantly with the elevation of [Ca2+]i in the presence and absence of extracellular Ca2+. Irsogladine (10(-5) M), which has been shown to facilitate intercellular communication, suppressed the ionomycin-induced elevation of [Ca2+]i and decrease in intercellular communication. The suppression of the ionomycin effects by irsogladine was independent of extracellular Ca2+. TMB-8 [8-(diethylamino)octyl-3,4,5-trimethoxy-benzoate hydrochloride] (10(-6) M) also suppressed the ionomycin-induced elevation of [Ca2+]i and decrease in intercellular communication. These results indicate that the ionomycin-induced decrease in intercellular communication may be due to Ca2+ mobilization from intracellular stores. Inhibitory effects of irsogladine and TMB-8 on the ionomycin-induced decrease in intercellular communication may be produced by suppressing Ca2+ mobilization.
Kameda
Y
Y
Research Laboratories, Nippon Shinyaku Co., Ltd., Kyoto, Japan.
Ueda
F
F
D000818:Animals; D000897:Anti-Ulcer Agents / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism*; D002452:Cell Count / Q000187:drug effects; D002478:Cells, Cultured / Q000187:drug effects; D004533:Egtazic Acid / Q000494:pharmacology; D004848:Epithelium / Q000187:drug effects; D015759:Ionomycin / Q000494:pharmacology*; D011817:Rabbits; D013270:Stomach / Q000187:drug effects*; D013997:Time Factors; D014227:Triazines / Q000494:pharmacology*
D000897:Anti-Ulcer Agents; D014227:Triazines; D004533:Egtazic Acid; D015759:Ionomycin; C041836:irsogladine; D002118:Calcium