title: Electric myocardial impedance registration in humoral rejection after heart transplantation.
abstract: BACKGROUND
  Because of the absent lymphocyte infiltrate, humoral-mediated rejection after heart transplantation is not diagnosed by the usual staining technique (hematoxylin-eosin method) of the endomyocardial biopsy specimen. However, humoral rejection is characterized by a distinct myocardial edema caused by capillary leakage. Because tissue edema increases the electric myocardial impedance of the corresponding tissue compartment the electric myocardial impedance method should be able to detect these episodes more reliably than biopsy.
  
  
  METHODS
  To evaluate this hypothesis eight DLA-matched beagle dogs were subjected to heterotopic neck heart transplantation after multiple sensitization by skin grafts of the heart donor. For electric myocardial impedance registrations rectangular impulses (wide 1 msec) were applied over two intramyocardial electrodes and the impulse response was registered. Day-to-day comparisons were made and an increase of electric myocardial impedance of 10% or more was used as an indicator of rejection. To assess the influence of edema caused by electrode implantation, cortisone administration, narcosis, ischemia, or reperfusion on the electric myocardial impedance, identical electrodes were implanted in the native hearts of five additional dogs via lateral thoracotomy. These animals each received 100 mg methylprednisolone between postoperative days 20 and 22 and underwent heterotopic neck heart transplantation on postoperative day 28 without previous sensitization (protocol 2). Electric myocardial impedance electrodes were also implanted in these allografts (protocol 3). After transplantation myocardial biopsies were done every 2 days and the samples graded according to the International Society for Heart and Lung Transplantation classification in all dogs.
  
  
  RESULTS
  Despite triple-drug immunosuppression (cyclosporine A, prednisolone, azathioprine) humoral rejection developed in all sensitized dogs as established by immunofluorescent staining of myocardial biopsy samples and functional deterioration. All episodes were diagnosed by electric myocardial impedance (sensitivity 100%), whereas only in one case the biopsy specimen was positive (International Society for Heart and Lung Transplantation grade > 1) (sensitivity 12.5%). All eight episodes could be treated successfully, that is, myocardial performance and electric myocardial impedance showed an immediate and full recovery. During the first 12 days none of the nonsensitized dogs exhibited rejection. Protocol 2 indicated that narcosis and the administration of cortisone did not per se have an influence on electric myocardial impedance and the influence of electrode implantation was negligible. Contrarily, edema caused by ischemia and reperfusion during transplantation (protocols 1 and 3) led to a significant increase in electric myocardial impedance. However, after 2 days this edema had faded away such that it no longer disturbed rejection diagnosis.
  
  
  CONCLUSIONS
  We conclude that the registration of the electric myocardial impedance diagnoses humoral rejection episodes after heart transplantation not only reliably but also early, that is, before the onset of irreversible graft damage.
authors.affiliation: German Heart Institute, Berlin, Germany.
authors.forename: O
authors.initials: O
authors.lastname: Grauhan
authors.forename: J
authors.initials: J
authors.lastname: Müller
authors.forename: C
authors.initials: C
authors.lastname: Knosalla
authors.forename: T
authors.initials: T
authors.lastname: Cohnert
authors.forename: H
authors.initials: H
authors.lastname: Siniawski
authors.forename: H D
authors.initials: HD
authors.lastname: Volk
authors.forename: E
authors.initials: E
authors.lastname: Fietze
authors.forename: W
authors.initials: W
authors.lastname: Kupetz
authors.forename: R
authors.initials: R
authors.lastname: Hetzer
mesh_terms: D000818:Animals; D000917:Antibody Formation / Q000276:immunology*; D001706:Biopsy; D002199:Capillary Permeability / Q000187:drug effects / Q000276:immunology; D002307:Cardiography, Impedance / Q000295:instrumentation; D004285:Dogs; D004359:Drug Therapy, Combination; D004452:Echocardiography / Q000187:drug effects; D004487:Edema / Q000276:immunology / Q000473:pathology; D004567:Electrodes, Implanted; D006084:Graft Rejection / Q000175:diagnosis* / Q000276:immunology / Q000473:pathology; D016027:Heart Transplantation / Q000276:immunology* / Q000473:pathology; D007166:Immunosuppressive Agents / Q000494:pharmacology; D009206:Myocardium / Q000276:immunology / Q000473:pathology; D013997:Time Factors; D016042:Transplantation, Heterotopic / Q000276:immunology* / Q000473:pathology
chemical_list: D007166:Immunosuppressive Agents