title: Cloning and sequencing of cDNAs encoding the human sperm protein, Sp17.
abstract: In the present study we have cloned and sequenced two testis-specific cDNAs (1.3 kb and 1.6 kb) encoding a human sperm protein, designated HSp17. Each cDNA gave rise to identical protein sequences and differed only in the 5' untranslated region. The predicted amino-acid sequence revealed a protein of 17.5 kDa which exhibited a high degree of homology with both rabbit and mouse Sp17. Analysis of native and recombinant Sp17 by SDS-PAGE has shown the apparent molecular weight of the protein to be 24.5 kDa.
authors.affiliation: Department of Cell Biology and Anatomy, University of North Carolina at Chapel Hill 27599, USA. ialea@email.unc.edu
authors.forename: I A
authors.initials: IA
authors.lastname: Lea
authors.forename: R T
authors.initials: RT
authors.lastname: Richardson
authors.forename: E E
authors.initials: EE
authors.lastname: Widgren
authors.forename: M G
authors.initials: MG
authors.lastname: O'Rand
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry*; D006801:Humans; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D011506:Proteins / Q000737:chemistry / Q000235:genetics*; D011817:Rabbits; D013094:Spermatozoa / Q000737:chemistry*
chemical_list: D018076:DNA, Complementary; D011506:Proteins