title: [Certain features of monoclonal rheumatoid immunoglobulin M fragmentation by "hot" trypsin].
abstract: Using analytical ultracentrifugation and affinity chromatography, it has been shown that "hot" trypsin (hydrolysis at 58-60 degrees C) releases two types of fragments from the monoclonal (Waldenstrom disease) immunoglobulin M possessing a rheumatoid activity (IgM-RF). The first fragment corresponds to a normal Fab-fragment as can be judged from its molecular weight. The other fragment designated as a (Fc)*5-fragment has a much higher molecular weight as compared with typical (Fc)*5-fragment released from non-rheumatoid IgM. According to calculations, the (Fc)*5-fragment retains two uncleaved Fab-regions and displays a rheumatoid activity. The Fab-fragments pool cleaved from IgM-RF consists of non-rheumatoid and rheumatoid components at an approximate ratio of 3:1. It seems, therefore, likely, that ten Fab-regions of IgM-RF are nonidentical with regard to their functional and structural properties. Most of those do not possess a rheumatoid activity and are more readily cleaved from IgM-RF by "hot" trypsin in comparison with rheumatoid active fragments.
authors.affiliation: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow.
authors.forename: V A
authors.initials: VA
authors.lastname: Lapuk
authors.forename: V Ia
authors.initials: VIa
authors.lastname: Cherniak
authors.forename: N N
authors.initials: NN
authors.lastname: Magretova
mesh_terms: D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000378:metabolism*; D002846:Chromatography, Affinity; D006801:Humans; D006868:Hydrolysis; D007140:Immunoglobulin Fab Fragments / Q000737:chemistry / Q000378:metabolism; D007075:Immunoglobulin M / Q000378:metabolism; D008970:Molecular Weight; D012217:Rheumatoid Factor / Q000737:chemistry / Q000378:metabolism*; D014357:Trypsin / Q000378:metabolism*; D014461:Ultracentrifugation
chemical_list: D000911:Antibodies, Monoclonal; D007140:Immunoglobulin Fab Fragments; D007075:Immunoglobulin M; D012217:Rheumatoid Factor; D014357:Trypsin
vernacular_title: Nekotorye osobennosti fragmentatsii monklonal'nogo revmatoidnogo immunoglobulina M "goriachim" tripsinom.