title: Antiviral and antiphlogistic activities of Hamamelis virginiana bark.
abstract: A crude hydroalcoholic extract from Hamamelis virginiana bark was subjected to ultrafiltration (UF) with a cut-off limit of 3 kDa to obtain a higher and a lower molecular weight fraction. Characterisation of the fractions was attempted with TLC, HPLC, acidic hydrolysis, and chromatography over Sephadex LH-20. The UF-concentrate was shown to consist mainly of oligomeric to polymeric proanthocyanidins (PA). This fraction was found to exhibit significant antiviral activity against Herpes simplex virus type 1 (HSV-1). In addition, the UV-concentrate displayed radical scavenging properties, inhibited alpha-glucosidase as well as human leukocyte elastase (HLE), and exhibited strong antiphlogistic effects in the croton oil ear edema test in the mouse. With the exception of the antioxidant potential and the inhibition of HLE-action the lower molecular fraction possessed weaker activities and contained mainly hamamelitannin, catechin, and further, unidentified constituents.
authors.affiliation: Dr. Willmar Schwabe Arzneimittel, Research and Development, Karlsruhe, Germany.
authors.forename: C A
authors.initials: CA
authors.lastname: Erdelmeier
authors.forename: J
authors.initials: J
authors.lastname: Cinatl
authors.forename: H
authors.initials: H
authors.lastname: Rabenau
authors.forename: H W
authors.initials: HW
authors.lastname: Doerr
authors.forename: A
authors.initials: A
authors.lastname: Biber
authors.forename: E
authors.initials: E
authors.lastname: Koch
mesh_terms: D000163:Acquired Immunodeficiency Syndrome; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000302:isolation & purification* / Q000494:pharmacology; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology; D000998:Antiviral Agents / Q000302:isolation & purification* / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D003436:Croton Oil; D004791:Enzyme Inhibitors / Q000302:isolation & purification* / Q000494:pharmacology; D065089:Glycoside Hydrolase Inhibitors; D018259:Herpesvirus 1, Human / Q000187:drug effects* / Q000302:isolation & purification; D006801:Humans; D007249:Inflammation / Q000139:chemically induced / Q000188:drug therapy; D007668:Kidney; D019272:Leukocyte Elastase; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D010196:Pancreatic Elastase / Q000037:antagonists & inhibitors*; D010936:Plant Extracts / Q000494:pharmacology*; D018547:Plant Stems; D010946:Plants, Medicinal; D014462:Ultrafiltration
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D000975:Antioxidants; D000998:Antiviral Agents; D004791:Enzyme Inhibitors; D065089:Glycoside Hydrolase Inhibitors; D010936:Plant Extracts; D003436:Croton Oil; D010196:Pancreatic Elastase; D019272:Leukocyte Elastase