Structural differences between the free and bound states of the DNA-bisintercalating peptide YSPTSPSY.
The YSPTSPSY peptide is a DNA-bisintercalator that can adopt nonrandom conformations in solution. Strategies based on random conformational search and energy minimizations have been applied to generate populations of conformers characterizing YSPTSPSY. Subsequent analysis based on statistical methods and clustering allowed to determine the existence of four classes of conformers containing beta- and/or gamma-turns. NMR spectra of YSPTSPSY in solution provide evidence for such structures. Employing a Monte Carlo-based docking procedure, the YSPTSPSY peptide was docked in a DNA double-helical fragment with the sequence [d(GACGTC)]2. The peptide binds on the minor groove of DNA stacking the central CG base pairs, in a manner similar to that observed in complexes of triostin A with DNA. Upon binding, the structure of the C-terminal segment is modified into a type I beta-turn. Five intermolecular hydrogen bonds are observed, but the van der Waals interactions constitute the major stabilization factor for the complex. NMR chemical shifts, coupling constants, and NOESY connectivities are in agreement with the molecular model.
Khiat
A
A
INRS-Santé, Université du Québec, Pointe-Claire, Canada.
Lamoureux
M
M
Boulanger
Y
Y
D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000378:metabolism; D001482:Base Composition; D004247:DNA / Q000378:metabolism*; D006860:Hydrogen Bonding; D007364:Intercalating Agents / Q000737:chemistry* / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009010:Monte Carlo Method; D009842:Oligopeptides / Q000737:chemistry* / Q000378:metabolism* / Q000494:pharmacology; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D011810:Quinoxalines / Q000737:chemistry / Q000378:metabolism
D000900:Anti-Bacterial Agents; D007364:Intercalating Agents; D009842:Oligopeptides; D011810:Quinoxalines; C078883:tyrosyl-seryl-prolyl-threonyl-seryl-prolyl-seryl-tyrosine; C031945:triostin A; D004247:DNA