Constitutively enhanced nbl expression is associated with the induction of internucleosomal DNA cleavage by actinomycin D.
Previous studies have found nbl expression to transiently rise and fall during glucocorticoid-induced thymic apoptosis. This induction of apoptosis is blocked by the transcriptional inhibitor actinomycin D. However, actinomycin D can trigger apoptosis in other cell types, e.g., HL-60 cells. This study found that internucleosomal DNA cleavage typical of apoptosis is induced by actinomycin D in cell lines such as HL-60 which constitutively express high levels of nbl above a certain "threshold." In contrast, "DNA ladder" formation was not induced by actinomycin D in cell lines with low constitutive nbl expression. Enhanced nbl expression therefore appears to be associated with apoptosis which is either blocked or induced by actinomycin D.
Naora
H
H
Research School of Biological Sciences, Australian National University, Canberra, Australia.
Nishida
T
T
Shindo
Y
Y
Adachi
M
M
Naora
H
H
D000650:Amnion; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002460:Cell Line; D004247:DNA / Q000187:drug effects; D015342:DNA Probes; D004273:DNA, Neoplasm / Q000187:drug effects; D003609:Dactinomycin / Q000494:pharmacology*; D015870:Gene Expression / Q000187:drug effects; D018922:HL-60 Cells; D006801:Humans; D007700:Kinetics; D015458:Leukemia, T-Cell; D008099:Liver; D016403:Lymphoma, Large B-Cell, Diffuse; D016399:Lymphoma, T-Cell; D051379:Mice; D009363:Neoplasm Proteins / Q000096:biosynthesis*; D009707:Nucleosomes / Q000187:drug effects / Q000502:physiology*
D015342:DNA Probes; D004273:DNA, Neoplasm; D009363:Neoplasm Proteins; D009707:Nucleosomes; D003609:Dactinomycin; D004247:DNA