Expression of acylphosphatase in Saccharomyces cerevisiae enhances ethanol fermentation rate.
Previous experiments in vitro have demonstrated the ability of acylphosphatase to increase the rate of glucose fermentation in yeast. To evaluate the possibility of increasing fermentation in vivo also, a chemically synthesized DNA sequence coding for human muscle acylphosphatase was expressed at high level in Saccharomyces cerevisiae. Ethanol production was measured in these engineered strains in comparison with a control. Acylphosphatase expression strongly increased the rate of ethanol production both in aerobic and anaerobic culture. This finding may be potentially important for the development of more efficient industrial fermentation processes.
Raugei
G
G
Dipartimento Scienze Biochimiche, Università di Firenze, Italy.
Modesti
A
A
Magherini
F
F
Marzocchini
R
R
Vecchi
M
M
Ramponi
G
G
D017766:Acid Anhydride Hydrolases / Q000096:biosynthesis / Q000235:genetics*; D050260:Carbohydrate Metabolism; D004247:DNA / Q000737:chemistry / Q000235:genetics; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D008965:Molasses; D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics; D000097024:Acylphosphatase
D017766:Acid Anhydride Hydrolases; D004247:DNA; D000431:Ethanol; D000097024:Acylphosphatase