Membrane changes associated with the temperature-sensitive P85gag-mos-dependent transformation of rat kidney cells as determined by dielectrophoresis and electrorotation.
Conventional dielectrophoresis (cDEP) and electrorotation (ROT) measurements have been used to determine the dielectric properties of a clone of normal rat kidney cells, designated 6m2, that exhibits a transformed phenotype at 33 degrees C and a non-transformed phenotype at 39 degrees C. cDEP measurements of the crossover frequencies at which individual 6m2 cells experienced zero cDEP force performed as a function of the conductivity of the suspension medium revealed that, in response to a temperature shift from 33 degrees C to 39 degrees C for 24 h, the mean specific cell membrane capacitance and conductance fell significantly (P < 0.01) from 42.3 (+/-1.3) to 30.3 (+/-2.9) mF/m2 and 743 (+/-422) to 567 (+/-326) S/m2, respectively. ROT analyses demonstrated a similar reduction for the membrane capacitance from 37.2 (+/-7.3) to 27.4 (+/-6.1) mF/m2, and also showed that accompanying changes in the mean internal electrical conductivity and dielectric permittivity of the cells were insignificant. Scanning electron microscopy was used to examine the surface morphology of the cells and, in agreement with our previous reports for leukemia cells, the observed membrane capacitance values correlated closely with the morphological complexity of the cell membrane surface. The observed changes in the membrane dielectric properties are discussed in terms of their biological significance and their relationship to previously-detected changes in cell surface charge.
Huang
Y
Y
Department of Molecular Pathology, University of Texas, MD Anderson Cancer Center, Houston 77030, USA.
Wang
X B
XB
Becker
F F
FF
Gascoyne
P R
PR
D000818:Animals; D002461:Cell Line, Transformed; D002462:Cell Membrane / Q000502:physiology* / Q000648:ultrastructure; D004553:Electric Conductivity; D004563:Electrochemistry; D004566:Electrodes; D004586:Electrophoresis / Q000379:methods*; D004594:Electrophysiology; D007668:Kidney / Q000648:ultrastructure*; D008855:Microscopy, Electron, Scanning; D051381:Rats; D013696:Temperature