Eimeria tenella: infection with a single sporocyst gives a clonal population.
Two genetically defined parents of Eimeria tenella, each characterized by a selectable phenotype, were mated to provide "recombinant' oocysts for an analysis of the inheritance of DNA markers into the sporocyst stage of the life-cycle. A total of 23 populations was established from infection with a putative single sporocyst and their inheritance of DNA markers provided compelling evidence that the 2 sporozoites within each sporocyst are genetically identical. EAch of the 5 polymorphic DNA markers used segregated independently within the "recombinant' parasites and only 1 of the parental forms was inherited in 22 of the populations aimed to have been established from a single sporocyst.
Shirley
M W
MW
Institute for Animal Health, Compton Laboratory, Nr Newbury, Berks, UK. shirley@bbsrc.ac.uk.
Harvey
D A
DA
D000225:Adenine / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D002645:Chickens; D003049:Coccidiostats / Q000494:pharmacology; D003433:Crosses, Genetic; D004351:Drug Resistance; D016786:Eimeria tenella / Q000235:genetics* / Q000254:growth & development*; D005819:Genetic Markers; D010641:Phenotype
D003049:Coccidiostats; D005819:Genetic Markers; C016245:arprinocid; D000225:Adenine