Cloning, characterization, and regulation of nifF from Rhodobacter capsulatus.
The Rhodobacter capsulatus nifF gene and upstream sequence were cloned by using a probe based on the N-terminal sequence of NifF. nifF was found to not be contained in the previously described nif regions I, II, and III. Comparison of the deduced amino acid sequence showed that it is highly similar to NifF from Azotobacter vinelandii and NifF from Klebsiella pneumoniae. Analysis of translational fusions demonstrated that the regulation of transcription was the same as previously reported at the protein level. Insertional mutagen esis showed that NifF contributes significantly to nitrogenase activity under normal nitrogen-fixing conditions and that it is absolutely required for nitrogen fixation under iron limitation.
Gennaro
G
G
Département de Microbiologie et Immunologie, Université de Montréal, Canada.
Hübner
P
P
Sandmeier
U
U
Yakunin
A F
AF
Hallenbeck
P C
PC
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial; D005418:Flavodoxin / Q000235:genetics*; D005798:Genes, Bacterial; D007763:Lac Operon; D008969:Molecular Sequence Data; D009154:Mutation; D009586:Nitrogen Fixation / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000235:genetics; D016354:Rhodobacter capsulatus / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
D004269:DNA, Bacterial; D005418:Flavodoxin; D011993:Recombinant Fusion Proteins