Applications of heteroduplex analysis for mutation detection in disease genes.
Double-stranded heteroduplex molecules that form between a mutant and wild-type DNA strand are often distinguished from homoduplex molecules upon gel electrophoresis. This method, heteroduplex analysis (HA), can be performed rapidly without radioisotopes or specialized equipment. Modifications and enhancements of the HA method have been developed that increase the sensitivity of detection of single-base pair alterations.
Glavac
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D
National Institute of Chemistry, Ljubljana, Slovenia.
Dean
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M
D003550:Cystic Fibrosis / Q000235:genetics; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000235:genetics; D004252:DNA Mutational Analysis / Q000379:methods*; D005814:Genes, Viral; D005820:Genetic Testing / Q000379:methods*; D015488:HIV Antigens / Q000235:genetics; D006650:Histocompatibility Testing; D006801:Humans; D009154:Mutation; D009692:Nucleic Acid Heteroduplexes / Q000032:analysis* / Q000235:genetics; D018807:Polymorphism, Single-Stranded Conformational; D012680:Sensitivity and Specificity
C505032:CFTR protein, human; D015488:HIV Antigens; D009692:Nucleic Acid Heteroduplexes; D019005:Cystic Fibrosis Transmembrane Conductance Regulator