An epidemiological study on viral infantile diarrhoea in Tirana.
During the period May 1993-April 1994, an epidemiological survey was conducted on enteric viruses which cause gastroenteritis in infants and young children in Tirana, Albania. Specimens from 321 cases were screened by direct electron microscopy and by an enzyme-linked immunosorbent assay specific for rotavirus group A antigen. By ultrastructural analysis, rotaviruses were detected in 10.3% of cases and adenoviruses in 0.6%, whereas small round structured viruses and small round viruses were found in 2.8% and 2.2% of cases, respectively. Different percentages of rotavirus excretors were revealed by enzyme-linked immunosorbent assay (12.15%) and electron microscopy. Samples rotavirus-positive in at least one of these assays were also analyzed by agglutination of latex particles and electron microscopy results were confirmed. Analysis of electron microscopy-positive samples by rotaviral RNA polyacrylamide gel electrophoresis showed five different long electropherotypes of rotavirus among which a single, largely predominant electropherotype (65.5%) was observed.
Diamanti
E
E
Department of Epidemiology, Research Institute of Hygiene, Tirana, Albania.
Superti
F
F
Tinari
A
A
Marziano
M L
ML
Giovannangeli
S
S
Tafaj
F
F
Xhelili
L
L
Gani
D
D
Donelli
G
G
D000256:Adenoviridae / Q000302:isolation & purification; D000415:Albania / Q000453:epidemiology; D000956:Antigens, Viral / Q000302:isolation & purification; D002675:Child, Preschool; D003968:Diarrhea, Infantile / Q000453:epidemiology* / Q000821:virology; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000821:virology; D005759:Gastroenteritis / Q000453:epidemiology* / Q000821:virology; D006306:Health Surveys; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D012367:RNA, Viral / Q000302:isolation & purification; D012401:Rotavirus / Q000302:isolation & purification; D012400:Rotavirus Infections / Q000453:epidemiology*; D014777:Virus Diseases / Q000453:epidemiology*; D014780:Viruses / Q000302:isolation & purification
D000956:Antigens, Viral; D012367:RNA, Viral