The humoral immune response of turbot to recently isolated pathogenic Enterococcus strains. Cross-reactivity with other Gram-positive bacteria.
An Enterococcus sp. causing severe mortalities among farmed turbot (Scophthalmus maximus) has recently been detected in northwest Spain. We found that specific turbot serum antibodies raised against isolate RA-99.1 of the new pathogen by intraperitoneal immunization, did not cross react in enzyme-linked immunosorbent assay (ELISA) with other enterococcal or non-enterococcal Gram-positive bacteria. In immunoblotting, antibodies raised against strain RA-99.1 recognized the same components in the homologous total soluble antigen preparation (TSA) as in TSA of two other isolates of the pathogen obtained from different farms. Anti-RA-99.1 serum also recognized some components of the TSA of several other Gram-positive bacteria. Immunogold labelling indicated that the antigens which provoke the humoral immune response to this pathogen are located mainly on the bacterial surface.
Leiro
J
J
Departamento de Microbiología y Parasitología, Universidad de Santiago de Compostela, Spain.
Toranzo
A E
AE
Estevez
J
J
Lamas
J
J
Barja
J L
JL
Ubeira
F M
FM
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D003429:Cross Reactions; D016983:Enterococcus / Q000276:immunology*; D005393:Fish Diseases / Q000276:immunology* / Q000382:microbiology; D005412:Flatfishes / Q000276:immunology* / Q000382:microbiology; D016908:Gram-Positive Bacterial Infections / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D008854:Microscopy, Electron / Q000662:veterinary
D000907:Antibodies, Bacterial