title: The cloning and characterization of a cDNA encoding Xenopus laevis DNA ligase I.
abstract: A cDNA clone coding for DNA ligase I (LigI) was isolated from a Xenopus laevis oocyte cDNA library. The 3766-bp sequence showed a putative ORF capable of encoding a 1070-amino-acid protein whose overall identity with two mammalian sequences is 63%. This identity, however, rises to 72.5% in the C-terminal portion of the protein that contains the active site. Expression of the cDNA in a prokaryotic system produces a protein that is immunologically identical to LigI and can be adenylated. The 180-kDa size of the recombinant protein is similar to the LigI detected in oocyte. Northern blot analysis of ovary and embryo RNAs revealed the expression of two (4.1 and 6 kb) LigI transcripts.
authors.affiliation: Département de Biologie et Génétique du Développement, URA 256 CNRS, Université de Rennes I, France.
authors.forename: D
authors.initials: D
authors.lastname: Lepetit
authors.forename: P
authors.initials: P
authors.lastname: Thiebaud
authors.forename: S
authors.initials: S
authors.lastname: Aoufouchi
authors.forename: C
authors.initials: C
authors.lastname: Prigent
authors.forename: R
authors.initials: R
authors.lastname: Guesné
authors.forename: N
authors.initials: N
authors.lastname: Thézé
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D000072481:DNA Ligase ATP; D011088:DNA Ligases / Q000235:genetics* / Q000378:metabolism; D018076:DNA, Complementary; D004625:Embryo, Nonmammalian / Q000201:enzymology; D004926:Escherichia coli; D008969:Molecular Sequence Data; D014982:Xenopus laevis
chemical_list: D018076:DNA, Complementary; D011088:DNA Ligases; D000072481:DNA Ligase ATP