title: Polylysine enhances cationic liposome-mediated transfection of the hepatoblastoma cell line Hep G2.
abstract: Plasmid DNA condensed by polylysine enhanced cationic-liposome-mediated transfection of Hep G2 cells. The luciferase expression plasmid pCMVL was complexed with the polycation poly-L-lysine and mixed with liposomes that contained a 1:1 molar ratio of the cationic lipid 1,2-dioleoyloxy-3-trimethyl-ammoniumpropane, with the neutral phospholipid 1,2-di-(cis-9-octadecenoyl)-sn-glycero-3-phosphoethanolamine. Polylysine enhanced cationic-liposome-mediated transfection of the hepatoblastoma cell line Hep G2 9-fold compared with pCMVL complexed alone with liposomes. The ratio of cationic to anionic charge of the polylysine-pCMVL complexes, and the quantity of cationic liposomes, are important determinants for optimal transfection of Hep G2 cells.
authors.affiliation: Department of Microbiology, University of California, Davis 95616, USA.
authors.forename: K D
authors.initials: KD
authors.lastname: Mack
authors.forename: R L
authors.initials: RL
authors.lastname: Walzem
authors.forename: K
authors.initials: K
authors.lastname: Lehmann-Bruinsma
authors.forename: J S
authors.initials: JS
authors.lastname: Powell
authors.forename: J B
authors.initials: JB
authors.lastname: Zeldis
mesh_terms: D002412:Cations; D004587:Electrophoresis, Agar Gel; D005229:Fatty Acids, Monounsaturated / Q000737:chemistry / Q000378:metabolism; D018197:Hepatoblastoma / Q000473:pathology*; D006801:Humans; D008081:Liposomes; D008113:Liver Neoplasms / Q000473:pathology*; D008156:Luciferases / Q000378:metabolism; D010316:Particle Size; D010714:Phosphatidylethanolamines / Q000737:chemistry / Q000378:metabolism; D010957:Plasmids / Q000378:metabolism; D011107:Polylysine / Q000378:metabolism / Q000494:pharmacology*; D000644:Quaternary Ammonium Compounds / Q000737:chemistry / Q000378:metabolism; D014162:Transfection; D014407:Tumor Cells, Cultured
chemical_list: D002412:Cations; D005229:Fatty Acids, Monounsaturated; D008081:Liposomes; D010714:Phosphatidylethanolamines; D000644:Quaternary Ammonium Compounds; D011107:Polylysine; C020888:1,2-dielaidoylphosphatidylethanolamine; D008156:Luciferases; C070046:1,2-dioleoyloxy-3-(trimethylammonium)propane