The inflammatory macrophage response to MCMV in mice with a retroviral immunodeficiency syndrome (MAIDS).
We have shown that normal C57BL/6J mice are moderately resistant to infection with murine cytomegalovirus (MCMV) and that this resistance is impaired by prior infection with LP-BM5 MuLV, which causes a disease (MAIDS) similar to early HIV-induced disease. This study investigates macrophage function in MAIDS+ mice challenged with MCMV. MAIDS reduces the influx of cells into the peritoneal cavity seen in normal C57BL/6J mice 6 days after MCMV infection. The infiltrates contained cells that resembled activated macrophages, as they took up colloidal gold, expressed the macrophage marker Mac-1, had high levels of acid phosphatase activity, and were lymphocytostatic when co-cultured with activated T cells. MAIDS+ mice had a higher percentage of cells able to take up colloidal gold and higher acid phosphatase activity per cell. The cells were also more lymphocytostatic and produced higher levels of interleukin-1 and tumor necrosis factor-alpha on days 4 and 6 after MCMV infection. Hence, MAIDS enhances baseline and induced macrophage activity, but depresses infiltration into the site of inflammation.
Allcock
R J
RJ
Department of Microbiology, University of Western Australia, Nedlands, Australia.
Peacock
C D
CD
Price
P
P
D000135:Acid Phosphatase / Q000032:analysis; D000818:Animals; D002478:Cells, Cultured; D018920:Coculture Techniques; D003587:Cytomegalovirus / Q000302:isolation & purification; D003586:Cytomegalovirus Infections / Q000150:complications* / Q000276:immunology; D005260:Female; D005434:Flow Cytometry; D007249:Inflammation; D007375:Interleukin-1 / Q000096:biosynthesis; D008213:Lymphocyte Activation; D008262:Macrophage Activation; D016177:Macrophage-1 Antigen / Q000032:analysis; D017737:Macrophages, Peritoneal / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D016183:Murine Acquired Immunodeficiency Syndrome / Q000150:complications* / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D007375:Interleukin-1; D016177:Macrophage-1 Antigen; D014409:Tumor Necrosis Factor-alpha; D000135:Acid Phosphatase