title: Molecular cloning and sequencing of a cDNA encoding dihydropyrimidinase from the rat liver.
abstract: A cDNA encoding dihydropyrimidinase has been isolated from a rat cDNA library. The N-terminal and an internal amino acid sequences were determined, and PCR primers were designed based on these sequences. Using a cDNA fragment amplified by RT-PCR with these primers, three cDNA clones were isolated from a rat liver library. The clone with the longest insert of 2129 bp contained a 1557 bp open reading frame encoding a polypeptide of 519 residues with a molecular mass of 56,832 Da.
authors.affiliation: Faculty of Nutrition, Kobe Gakuin University, Japan. kmatsuda@ipc.kobegakuin.ac.jp
authors.forename: K
authors.initials: K
authors.lastname: Matsuda
authors.forename: S
authors.initials: S
authors.lastname: Sakata
authors.forename: M
authors.initials: M
authors.lastname: Kaneko
authors.forename: N
authors.initials: N
authors.lastname: Hamajima
authors.forename: M
authors.initials: M
authors.lastname: Nonaka
authors.forename: M
authors.initials: M
authors.lastname: Sasaki
authors.forename: N
authors.initials: N
authors.lastname: Tamaki
mesh_terms: D000581:Amidohydrolases / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary; D008099:Liver / Q000201:enzymology*; D008969:Molecular Sequence Data; D051381:Rats; D017386:Sequence Homology, Amino Acid
chemical_list: D018076:DNA, Complementary; D000581:Amidohydrolases; C020047:dihydropyrimidinase