title: Detection of immunoglobulin G to a Sindbis-related virus by a membrane antigen enzyme immunoassay.
abstract: We determined the seroprevalence of a Sindbis-related virus isolated for the first time in 1975 from ticks in south-east Sicily and typed by Gresikova et al. in 1978. An indirect enzyme immunoassay based on viral membrane antigen for coating microtiter strips was used for the detection of immunoglobulin G to the Sindbis-related virus. The method appeared more sensitive than a similar enzyme immunoassay based on crude lysate antigen. Comparison of the results obtained from sera tested both by membrane antigen enzyme immunoassay and microneutralization test showed 92% agreement, while the agreement between microneutralization test and crude antigen enzyme immunoassay was 76%. An overall elevated seroprevalence (63.66%) was found in a population group living in and around the area of first isolation and seroprevalence in different age groups was also studied.
authors.affiliation: Institute of Microbiology, University of Catania, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Scalia
authors.forename: M
authors.initials: M
authors.lastname: Garufi
authors.forename: F
authors.initials: F
authors.lastname: Condorelli
authors.forename: A
authors.initials: A
authors.lastname: Stivala
authors.forename: M C
authors.initials: MC
authors.lastname: Costanzo
authors.forename: M
authors.initials: M
authors.lastname: Tiralongo
authors.forename: A D
authors.initials: AD
authors.lastname: Adragna
authors.forename: A
authors.initials: A
authors.lastname: Marino
authors.forename: S
authors.initials: S
authors.lastname: Guglielmino
authors.forename: G
authors.initials: G
authors.lastname: Tempera
authors.forename: A
authors.initials: A
authors.lastname: Castro
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D018354:Alphavirus Infections / Q000175:diagnosis* / Q000453:epidemiology; D000914:Antibodies, Viral / Q000097:blood*; D000956:Antigens, Viral / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D006801:Humans; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000097:blood; D007223:Infant; D008875:Middle Aged; D009500:Neutralization Tests; D011934:Reagent Strips; D012802:Sicily / Q000453:epidemiology; D012845:Sindbis Virus / Q000276:immunology*; D014763:Viral Matrix Proteins / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D007074:Immunoglobulin G; D011934:Reagent Strips; D014763:Viral Matrix Proteins