Deficient DNA repair capacity, a predisposing factor in breast cancer.
Women with breast cancer and a family history of breast cancer and some with sporadic breast cancer are deficient in the repair of radiation-induced DNA damage compared with normal donors with no family history of breast cancer. DNA repair was measured indirectly by quantifying chromatid breaks in phytohaemagglutinin (PHA)-stimulated blood lymphocytes after either X-irradiation or UV-C exposure, with or without post treatment with the DNA repair inhibitor, 1-beta-D-arabinofuranosylcytosine (ara-C). We have correlated chromatid breaks with unrepaired DNA strand breaks using responses to X-irradiation of cells from xeroderma pigmentosum patients with well-characterised DNA repair defects or responses of repair-deficient mutant Chinese hamster ovary (CHO) cells with or without transfected human DNA repair genes. Deficient DNA repair appears to be a predisposing factor in familial breast cancer and in some sporadic breast cancers.
Parshad
R
R
Department of Pathology, Howard University College of Medicine, Washington, DC 20059, USA.
Price
F M
FM
Bohr
V A
VA
Cowans
K H
KH
Zujewski
J A
JA
Sanford
K K
KK
D000818:Animals; D001943:Breast Neoplasms / Q000097:blood / Q000209:etiology* / Q000235:genetics; D016466:CHO Cells / Q000502:physiology / Q000528:radiation effects; D002842:Chromatids / Q000528:radiation effects; D006224:Cricetinae; D003561:Cytarabine / Q000494:pharmacology; D004249:DNA Damage; D004260:DNA Repair / Q000187:drug effects / Q000235:genetics / Q000528:radiation effects; D004273:DNA, Neoplasm / Q000528:radiation effects; D005260:Female; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects / Q000528:radiation effects; D008214:Lymphocytes / Q000187:drug effects / Q000528:radiation effects; D010835:Phytohemagglutinins / Q000494:pharmacology; D012307:Risk Factors; D014162:Transfection; D014466:Ultraviolet Rays; D014965:X-Rays; D014983:Xeroderma Pigmentosum / Q000378:metabolism / Q000473:pathology / Q000532:radiotherapy
D004273:DNA, Neoplasm; D010835:Phytohemagglutinins; D003561:Cytarabine