Alpha-fetoprotein-mediated targeting of anti-cancer drugs to tumor cells in vitro.
An oncofetal protein, human alpha-fetoprotein, was selected as a vector molecule for targeted delivery of antitumor substances. Conjugates of alpha-fetoprotein with doxorubicin, daunomycin, calichemicin, carboxyphosphamide, bleomycetin, chlorbutin, cis-platinum, methotrexate were synthesized. All conjugates displayed a highly selective antitumor cytotoxic activity towards human cell cultures. The optimal alpha-fetoprotein:cytotoxic compound ratio was shown to be 1:3 - 1:5. The results obtained serve as a basis for creating antitumor preparations of a new generation, which are highly selective to tumor cells owing to receptor-mediated endocytosis.
Severin
S E
SE
Moscow's Research Institute of Medical Ecology, Russia.
Moskaleva EYu
Shmyrev
I I
II
Posypanova
G A
GA
Belousova YuV
Sologub
V K
VK
Luzhkov YuM
Nakachian
R
R
Andreani
J
J
Severin
E S
ES
D000970:Antineoplastic Agents / Q000008:administration & dosage* / Q000737:chemistry; D016923:Cell Death / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D016503:Drug Delivery Systems; D006801:Humans; D014407:Tumor Cells, Cultured; D000509:alpha-Fetoproteins / Q000008:administration & dosage* / Q000737:chemistry
D000970:Antineoplastic Agents; D000509:alpha-Fetoproteins