Glycans of the trabecular meshwork in primary open angle glaucoma.
OBJECTIVE
Glycan expression was compared in glaucomatous trabecular meshwork (TM) and normal TM in order to determine any differences which may reflect pathological changes underlying primary open angle glaucoma (POAG).


METHODS
Resin embedded TM from trabeculectomy specimens from 15 eyes with POAG and from 12 eyes with normal anterior segments were probed with a panel of biotinylated lectins and an avidin-peroxidase revealing system at the light microscope level. Statistical analyses were performed on the comparative staining results.


RESULTS
The lectins ConA and ePHA showed strong staining in all areas of both glaucomatous and normal TM; ePHA staining of Schlemm's canal (SC) from POAG TM was significantly less than that from normal TM (ePHA-SC p = 0.04). The lectins PSA, LCA, and SNA bound moderately strongly to SC endothelium and weakly to the endothelium of the corneoscleral meshwork (CSM); glaucomatous SC endothelial binding was significantly less than that of normal SC endothelium for PSA and LCA (PSA-SC p = 0.002, LCA-SC p = 0.002). STA and DSA showed moderately strong binding while WGA, ECA, AHA, and MPA bound weakly throughout the TM; for DSA and MPA this staining was significantly greater in POAG than in normal TM (DSA-SC p = 0.001, DSA-CSM p = 0.002, MPA-SC p = 0.01, MPA-CSM p = 0.02). Jac stained strongly throughout the TM and showed no significant difference in POAG compared with normal TM (Jac-SC p = 0.6, Jac-CSM p = 1). 1PHA, SBA, DBA, CTA, UEA-1 and LTA did not bind to glaucomatous TM or normal TM. There were no age-related changes seen.


CONCLUSIONS
The expression of some complex and hybrid, bisected and non-bisected N-linked glycans is significantly diminished in glaucomatous TM compared with normal TM. Some glycans with multiple N-acetylglucosamine residues and O-linked glycans with terminal and subterminal galactosyl groups are significantly increased in POAG TM. Glycan expression does not change significantly with age in POAG or normal TM.
Chapman
S A
SA
Department of Pathological Sciences, University of Manchester.
Bonshek
R E
RE
Stoddart
R W
RW
O'Donoghue
E
E
Goodall
K
K
McLeod
D
D
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001710:Biotin / Q000031:analogs & derivatives / Q000378:metabolism*; D016022:Case-Control Studies; D015870:Gene Expression; D005902:Glaucoma, Open-Angle / Q000378:metabolism* / Q000473:pathology; D006651:Histocytochemistry; D006801:Humans; D037102:Lectins / Q000378:metabolism*; D008875:Middle Aged; D010835:Phytohemagglutinins / Q000378:metabolism; D011134:Polysaccharides / Q000378:metabolism*; D014129:Trabecular Meshwork / Q000378:metabolism* / Q000473:pathology; D014909:Wheat Germ Agglutinins / Q000378:metabolism
D037102:Lectins; D010835:Phytohemagglutinins; D011134:Polysaccharides; D014909:Wheat Germ Agglutinins; D001710:Biotin