Biological and molecular properties of a pathotype P-1 and a pathotype P-4 isolate of pea seed-borne mosaic virus.
Two isolates of pea seed-borne mosaic potyvirus, DPD1 and NY, were identified as pathotypes P-1 and P-4, respectively, by their infectivity on Pisum sativum L. lines homozygous for the recessive resistance genes sbm-1 and sbm-4. The two isolates differed in several biological characteristics. DPD1 induced transient vein clearing, downward rolling of leaflets and internode shortening on P. sativum, whereas NY only caused a slight growth reduction. DPD1 moved systemically in Chenopodium quinoa whereas NY was restricted to inoculated leaves. DPD1 was frequently transmitted by seeds whereas NY was rarely seed-transmitted: 24% and 0.3%, respectively, in P. sativum '549'. Both DPD1 and NY were transmitted by aphids (Myzus persicae), though a DAG triplet was not present in the N terminus of the coat protein. The nucleotide sequence and deduced amino acid sequence of NY were determined and compared to the corresponding sequences of DPD1.
Johansen
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Department of Microbiology, Oregon State University, Corvallis 97331-3804, USA. e.johansen@dips-Lyngby.dk
Keller
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KE
Dougherty
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WG
Hampton
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D002874:Chromosome Mapping; D003001:Cloning, Molecular; D018076:DNA, Complementary; D017343:Genes, Plant; D005808:Genes, Recessive; D016679:Genome, Viral; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D018532:Pisum sativum / Q000821:virology*; D010935:Plant Diseases / Q000235:genetics / Q000821:virology; D016133:Polymerase Chain Reaction; D017800:Potyvirus / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity*; D012639:Seeds; D014764:Viral Proteins / Q000235:genetics
D018076:DNA, Complementary; D014764:Viral Proteins