Induction of interleukin (IL)-8 gene expression by respiratory syncytial virus involves activation of nuclear factor (NF)-kappa B and NF-IL-6.
Respiratory syncytial virus (RSV) preferentially infects respiratory epithelium and is an important cause of lower respiratory tract infections in young children. RSV induces the production of interleukin (IL)-8 in airway epithelial cells; however, the mechanism of this induction is not known. To define the mechanism by which RSV induces IL-8 gene activation, A549 epithelial cells were transfected with plasmids containing serial deletions of the 5'-flanking region of the IL-8 gene and then exposed to RSV for 24 h. A positive cooperative effect of the binding sites for the transcription factors, nuclear factor (NF)-kappa B and NF-IL-6, was observed. Mutations in either region abates responsiveness of the promoter to RSV infection. RSV also increases activation of the NF-kappa B and NF-IL-6 transcription factors. These data suggest that RSV may increase IL-8 production in airway epithelium partly via activation of the transcription factors NF-kappa B and NF-IL-6.
Mastronarde
J G
JG
Division of Pulmonary, Critical Care, University of Iowa College of Medicine, Iowa City, USA.
He
B
B
Monick
M M
MM
Mukaida
N
N
Matsushima
K
K
Hunninghake
G W
GW
D001483:Base Sequence; D001665:Binding Sites; D022762:CCAAT-Enhancer-Binding Proteins; D004268:DNA-Binding Proteins / Q000378:metabolism*; D005786:Gene Expression Regulation; D016209:Interleukin-8 / Q000235:genetics*; D008969:Molecular Sequence Data; D016328:NF-kappa B / Q000378:metabolism*; D009687:Nuclear Proteins / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D011650:Pulmonary Alveoli / Q000821:virology*; D012136:Respiratory Syncytial Viruses / Q000254:growth & development*; D015533:Transcriptional Activation; D014407:Tumor Cells, Cultured
D022762:CCAAT-Enhancer-Binding Proteins; D004268:DNA-Binding Proteins; D016209:Interleukin-8; D016328:NF-kappa B; D009687:Nuclear Proteins