Upregulation of the APC gene product during neuronal differentiation of rat pheochromocytoma PC12 cells.
The adenomatous polyposis coli (APC) gene, the mutation of which is responsible for familial adenomatous polyposis and sporadic colorectal tumors, is highly expressed in the central nervous system. To elucidate the contribution of the APC protein to neuronal differentiation, changes in APC expression were examined during nerve growth factor (NGF)-induced differentiation of rat pheochromocytoma PC12 cells. The expression of APC gradually increased throughout the time course, in particular it increased markedly after 7 days of exposure to NGF. However, forced expression of APC did not induce neuronal differentiation of PC12 cells. These results suggest that the APC protein itself does not have the potential to induce neuronal differentiation, but rather is upregulated secondary to the differentiation of PC12 cells.
Dobashi
Y
Y
Department of Oncogene Research, Research Institute for Microbial Diseases, Osaka University, Japan.
Bhattacharjee
R N
RN
Toyoshima
K
K
Akiyama
T
T
D025601:Adenomatous Polyposis Coli Protein; D000310:Adrenal Gland Neoplasms; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D003598:Cytoskeletal Proteins / Q000096:biosynthesis*; D015870:Gene Expression / Q000187:drug effects; D017491:Genes, APC; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000096:biosynthesis; D009414:Nerve Growth Factors / Q000494:pharmacology*; D016501:Neurites / Q000187:drug effects / Q000502:physiology; D009474:Neurons / Q000166:cytology* / Q000378:metabolism; D016716:PC12 Cells; D010673:Pheochromocytoma; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D014162:Transfection
D025601:Adenomatous Polyposis Coli Protein; D003598:Cytoskeletal Proteins; D008164:Luminescent Proteins; D009414:Nerve Growth Factors; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins