title: Tomato Prf is a member of the leucine-rich repeat class of plant disease resistance genes and lies embedded within the Pto kinase gene cluster.
abstract: In tomato, resistance to Pseudomonas syringae pv. tomato (Pst) strains expressing the avirulence gene avrPto requires the presence of at least two host genes, designated Pto and Prf. Here we report that Prf encodes a protein with leucine-zipper, nucleotide-binding, and leucine-rich repeat motifs, as are found in a number of resistance gene products from other plants. prf mutant alleles (4) were found to carry alterations within the Prf coding sequence. A genomic fragment containing Prf complemented a prf mutant tomato line both for resistance to Pst strains expressing avrPto and for sensitivity to the insecticide Fenthion. Prf resides in the middle of the Pto gene cluster, 24 kb from the Pto gene and 500 bp from the Fen gene.
authors.affiliation: Department of Plant Biology, University of California, Berkeley 94720, USA.
authors.forename: J M
authors.initials: JM
authors.lastname: Salmeron
authors.forename: G E
authors.initials: GE
authors.lastname: Oldroyd
authors.forename: C M
authors.initials: CM
authors.lastname: Rommens
authors.forename: S R
authors.initials: SR
authors.lastname: Scofield
authors.forename: H S
authors.initials: HS
authors.lastname: Kim
authors.forename: D T
authors.initials: DT
authors.lastname: Lavelle
authors.forename: D
authors.initials: D
authors.lastname: Dahlbeck
authors.forename: B J
authors.initials: BJ
authors.lastname: Staskawicz
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D001483:Base Sequence; D018244:Chromosomes, Artificial, Yeast / Q000235:genetics; D003001:Cloning, Molecular; D003360:Cosmids / Q000235:genetics; D005816:Genetic Complementation Test; D007113:Immunity, Innate / Q000235:genetics; D016350:Leucine Zippers / Q000235:genetics*; D018551:Solanum lycopersicum / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D010935:Plant Diseases / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics*; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D010940:Plant Proteins; C120613:Prf protein, Lycopersicon esculentum; C078090:abiC protein, Lactococcus lactis; C091599:Fen protein, Lycopersicon esculentum; C084354:Pto protein, Lycopersicon; D017346:Protein Serine-Threonine Kinases