Characterization of small intestinal submucosa regenerated canine detrusor: assessment of reinnervation, in vitro compliance and contractility.
OBJECTIVE
We characterized small intestinal submucosa regenerated canine bladder.


METHODS
We subjected 15-month small intestinal submucosa regenerated canine bladder strips to in vitro muscle bath compliance, contractility testing and immunohistochemical staining.


RESULTS
Compliance studies demonstrated no significant difference between small intestinal submucosa regenerated and control bladders, which were 30-fold more compliant than native small intestinal submucosal graft material. Contractility studies demonstrated contractile responses and innervation similar to those of normal canine bladder. Afferent nerves were demonstrated through immunohistochemical techniques.


CONCLUSIONS
These characteristics further support the regenerative capacity of small intestinal submucosa and its potential use as a bladder augmentation material.
Kropp
B P
BP
Department of Urology, Riley Children's Hospital, Indiana University School of Medicine, Lafayette, USA.
Sawyer
B D
BD
Shannon
H E
HE
Rippy
M K
MK
Badylak
S F
SF
Adams
M C
MC
Keating
M A
MA
Rink
R C
RC
Thor
K B
KB
D000818:Animals; D004285:Dogs; D066298:In Vitro Techniques; D007413:Intestinal Mucosa / Q000637:transplantation*; D007421:Intestine, Small / Q000637:transplantation*; D009119:Muscle Contraction / Q000187:drug effects / Q000502:physiology*; D009130:Muscle, Smooth / Q000187:drug effects / Q000502:physiology*; D012038:Regeneration; D001743:Urinary Bladder / Q000294:innervation* / Q000502:physiology* / Q000601:surgery