Opposite actions of transforming growth factor-beta 1 on the gene expression of atrial natriuretic peptide biological and clearance receptors in a murine thymic stromal cell line.
The regulation of the gene expression of the atrial natriuretic peptide receptor (ANPR) subtypes, ANPR-A, ANPR-B, and ANPR-C, was investigated in a murine thymic stromal cell line, MRL 104.8a. When MRL 104.8a cells were cultured with transforming growth factor (TGF)-beta1, [125I]ANP binding sites increased with increasing dose of TGF-beta1. These binding sites were identified as ANPR-C by a displacement experiment with ANPR-C-specific ligand, C-ANF, and by the affinity cross-linking of the [125I]ANP binding sites with a chemical cross-linker to determine the molecular weight of the ANPR. This augmentation of the ANPR-C expression was elucidated to occur at the transcriptional level by Northern blot experiment, comparison of the relative amounts of mRNA by reverse transcription (RT)-PCR, and in vitro nuclear transcription assay. Conversely, the expression of the ANP biological receptors, ANPR-A and ANPR-B, was shown to be down-regulated by TGF-beta1. These data suggest that TGF-beta1 regulates the gene expression of ANPRs in the thymic stromal cells and that ANP and TGF-beta1 might affect the thymic stromal cell functions.
Agui
T
T
Institute for Experimental Animal Science, Nagoya City University Medical School, Aichi.
Xin
X
X
Cai
Y
Y
Shim
G
G
Muramatsu
Y
Y
Yamada
T
T
Fujiwara
H
H
Matsumoto
K
K
D000818:Animals; D009320:Atrial Natriuretic Factor / Q000096:biosynthesis / Q000235:genetics*; D001483:Base Sequence; D002478:Cells, Cultured; D015536:Down-Regulation / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects*; D051379:Mice; D008969:Molecular Sequence Data; D017461:Receptors, Atrial Natriuretic Factor / Q000096:biosynthesis / Q000145:classification / Q000235:genetics*; D017154:Stromal Cells / Q000187:drug effects / Q000502:physiology; D013950:Thymus Gland / Q000166:cytology / Q000187:drug effects* / Q000502:physiology*; D016212:Transforming Growth Factor beta / Q000494:pharmacology* / Q000502:physiology
D016212:Transforming Growth Factor beta; D009320:Atrial Natriuretic Factor; D017461:Receptors, Atrial Natriuretic Factor