Immunochemical and biochemical identification of the rice seed protein encoded by cDNA clone A3-12.
Previously isolated cDNA clone A3-12 that was expressed in E. coli as the fusion protein with Trp E showed immunoreactivity with the mouse antibody raised against isolated alpha-globulin from rice seed. The N-terminal amino acid sequences determined for the purified alpha-globulin and its tryptic peptides were identical with the deduced amino acid sequence reported, except for two residues at the protein N terminus. An error in the reported sequence was confirmed by re-sequencing the cDNA, the nucleotide sequence for the two N-terminal residues being shown to be CAGCTG and not CACGTG. Thus, the protein encoded by cDNA clone A3-12 was identified to be the major rice seed globulin, alpha-globulin, with an apparent molecular mass of 26kDa.
Nakase
M
M
Department of Applied Biological Sciences, School of Agricultural Sciences, Nagoya University, Japan.
Alvarez
A M
AM
Adachi
T
T
Aoki
N
N
Nakamura
R
R
Matsuda
T
T
D000510:Alpha-Globulins / Q000032:analysis* / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D000595:Amino Acid Sequence; D000818:Animals; D002852:Chromatography, Ion Exchange; D018076:DNA, Complementary; D015151:Immunoblotting; D051379:Mice; D008969:Molecular Sequence Data; D012275:Oryza / Q000737:chemistry*; D010940:Plant Proteins / Q000032:analysis* / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification
D000510:Alpha-Globulins; D018076:DNA, Complementary; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins