title: Determination of synthetic estrogens in illegal veterinary formulations by HPTLC and HPLC.
abstract: To determine the actual amount of diethylstilbestrol, hexestrol, and dienestrol in formulations such as pellets and oily injections that are illegally available on the Brazilian market, a simple methanol extraction is used for the analysis of the pellets and an ether extraction with Sephadex columns (for clean-up) is used for the oily injections. High-performance thin-layer chromatography is used for identification (as a qualitative and semiquantitative method), and high-performance liquid chromatography is used for quantitation. The results of the analysis show that all the formulations are not in accordance with the information listed on their labels.
authors.affiliation: Departmento de Análises Clínicas e Toxicology, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo.
authors.forename: E S
authors.initials: ES
authors.lastname: Nascimento
authors.forename: M C
authors.initials: MC
authors.lastname: Salvadori
authors.forename: L M
authors.initials: LM
authors.lastname: Ribeiro-Neto
mesh_terms: D000818:Animals; D001938:Brazil; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000706:statistics & numerical data; D004028:Dienestrol / Q000032:analysis; D004054:Diethylstilbestrol / Q000032:analysis; D004348:Drug Labeling; D045166:Estradiol Congeners / Q000032:analysis*; D006589:Hexestrol / Q000032:analysis; D013287:Illicit Drugs / Q000032:analysis; D007880:Legislation, Drug; D014730:Veterinary Medicine
chemical_list: D045166:Estradiol Congeners; D013287:Illicit Drugs; D006589:Hexestrol; D004054:Diethylstilbestrol; D004028:Dienestrol