Influence of freezing at different temperatures on the transcription activity of buffalo sperm chromatin.
The effect of freezing at different temperatures, -20 degrees C, -70 degrees C and -196 degrees C, on the transcription activity of buffalo sperm chromatin was investigated. The kinetic data of incorporation of 3H-UTP in pre-mRNA indicate that temperatures of -70 degrees C and -196 degrees C are the most favourable for preservation, since transcription activity gradually increases with time, reaching maximum values within 30 min. Freezing at -20 degrees C or preservation at 4 degrees C leads to a rapid decrease of transcriptional activity within 10 and 20 min, respectively. It is suggested that the optimal temperature for storage of buffalo spermatozoa is below -20 degrees C as judged by the transcription assay.
Pironcheva
G L
GL
Institute of Molecular Biology, Bulgarian Academy of Sciences, Sofia, Bulgaria.
Miteva
K
K
Vaisberg
C
C
Russev
G
G
D000818:Animals; D002020:Buffaloes / Q000235:genetics / Q000502:physiology*; D002843:Chromatin / Q000235:genetics*; D015925:Cryopreservation / Q000379:methods / Q000662:veterinary*; D008297:Male; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary*; D013094:Spermatozoa / Q000378:metabolism*; D013696:Temperature; D014158:Transcription, Genetic
D002843:Chromatin