title: Effect of morphine on interleukin-1 and tumor necrosis factor alpha production from mouse peritoneal macrophages in vitro.
abstract: OBJECTIVE
  To study the influence of morphine on lymphokine production from mouse peritoneal macrophages (PM phi).
  
  
  METHODS
  The productions of interleukin-1 (IL-1) and tumor necrosis factor (TNF-alpha) from PM phi under various concentrations of morphine with or without naloxone were measured by testing thymocyte proliferation and L929 cell lysis in vitro.
  
  
  RESULTS
  Both morphine (1 x 10(-8) - 1 x 10(-3) mol L-1) and naloxone (50 mumol L-1) inhibited IL-1 production from mouse PM phi primed by sodium thioglycolate. Naloxone did not block the inhibitory effect of morphine. TNF-alpha production from PM phi was inhibited by only high concentration (1 mmol L-1) of morphine which was not blocked by naloxone.
  
  
  CONCLUSIONS
  Morphine had a direct inhibitory effect on PM phi, which was not mediated by opioid receptor of PM phi.
authors.affiliation: Shanghai Institute of Materia Medica, Chinese Academy of Sciences, China.
authors.forename: T H
authors.initials: TH
authors.lastname: Bian
authors.forename: X F
authors.initials: XF
authors.lastname: Wang
authors.forename: X Y
authors.initials: XY
authors.lastname: Li
mesh_terms: D000818:Animals; D005260:Female; D005350:Fibroma / Q000473:pathology; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D007375:Interleukin-1 / Q000096:biosynthesis*; D017737:Macrophages, Peritoneal / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008813:Mice, Inbred ICR; D009020:Morphine / Q000494:pharmacology*; D009270:Naloxone / Q000494:pharmacology; D009292:Narcotic Antagonists / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000187:drug effects; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*
chemical_list: D007166:Immunosuppressive Agents; D007375:Interleukin-1; D009292:Narcotic Antagonists; D014409:Tumor Necrosis Factor-alpha; D009270:Naloxone; D009020:Morphine