title: Chemiluminescence emission during reactions between superoxide and selected aliphatic and aromatic halocarbons in aprotic media.
abstract: The reactions between superoxide free radical anion (.O2-) with the halocarbons CCl4. CHCl3, BrCH2CH2Br(EDB), decachlora-biphenyl (DCBP), and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in dimethyl sulphoxide (DMSO) results in the emission of chemiluminescence (CL). The chemiluminescence reactions are characterized as having biphasic second order kinetics, CL wavelengths between 350 nm and 650 nm, and exhibiting perturbation by chemicals reactive with singlet oxygen. These data suggest that singlet oxygen species are the excited state responsible for the light emissions. Polarographic studies confirm .O2- consumption and halide release in the reactions, while gas liquid chromatography and NBT reduction demonstrate the decomposition of the halocarbons into products. A chemiluminescent reaction mechanism is proposed involving reductive dehalogenation of the halocarbons and the generation of singlet oxygen. The significance of singlet oxygen generation is discussed with respect to a general mechanism for explaining the rapid initiation of lipid peroxidative membrane damage in halocarbon toxigenicity in animal and plant tissues.
authors.affiliation: Health Protection Section, Bowman Gray School of Medicine, Wake Forest University, Winston-Salem, NC 27157, USA.
authors.forename: A R
authors.initials: AR
authors.lastname: Shoaf
authors.forename: A U
authors.initials: AU
authors.lastname: Shaikh
authors.forename: J H
authors.initials: JH
authors.lastname: Ford
authors.forename: W C
authors.initials: WC
authors.lastname: Carlson
authors.forename: R H
authors.initials: RH
authors.lastname: Steele
mesh_terms: D000818:Animals; D002251:Carbon Tetrachloride / Q000737:chemistry*; D002725:Chloroform / Q000737:chemistry*; D004121:Dimethyl Sulfoxide; D007700:Kinetics; D015227:Lipid Peroxidation; D008163:Luminescent Measurements; D009580:Nitroblue Tetrazolium; D010100:Oxygen; D010944:Plants; D011048:Polarography / Q000379:methods; D011078:Polychlorinated Biphenyls / Q000737:chemistry*; D000072317:Polychlorinated Dibenzodioxins / Q000737:chemistry*; D026082:Singlet Oxygen; D013481:Superoxides / Q000737:chemistry*
chemical_list: D000072317:Polychlorinated Dibenzodioxins; D013481:Superoxides; D026082:Singlet Oxygen; D009580:Nitroblue Tetrazolium; D002725:Chloroform; D002251:Carbon Tetrachloride; D011078:Polychlorinated Biphenyls; C005381:decachlorobiphenyl; D010100:Oxygen; D004121:Dimethyl Sulfoxide