Molecular cloning and sequencing of a cDNA encoding dihydropyrimidinase from the rat liver.
A cDNA encoding dihydropyrimidinase has been isolated from a rat cDNA library. The N-terminal and an internal amino acid sequences were determined, and PCR primers were designed based on these sequences. Using a cDNA fragment amplified by RT-PCR with these primers, three cDNA clones were isolated from a rat liver library. The clone with the longest insert of 2129 bp contained a 1557 bp open reading frame encoding a polypeptide of 519 residues with a molecular mass of 56,832 Da.
Matsuda
K
K
Faculty of Nutrition, Kobe Gakuin University, Japan. kmatsuda@ipc.kobegakuin.ac.jp
Sakata
S
S
Kaneko
M
M
Hamajima
N
N
Nonaka
M
M
Sasaki
M
M
Tamaki
N
N
D000581:Amidohydrolases / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary; D008099:Liver / Q000201:enzymology*; D008969:Molecular Sequence Data; D051381:Rats; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D000581:Amidohydrolases; C020047:dihydropyrimidinase