[Protective effect of polysaccharide sulfate on human umbilical vein endothelial cell (HUVEC) injury in vitro].
The influence of four kinds of polysaccharide sulfate [heparin (Hep), low molecular weight heparin (LMWH), proglylene glucol mannate sulfate (MS871), chondroitin A sulfate (CAS)] on HUVEC injured by polycations and oxygen free radicals was investigated. The degree of injury was determined by checking the release of LDH to medium and 3H-TdR incorporation. The four kinds of polysaccharide sulfate were shown to protect cells from injury caused by polylysine concentration-dependently. The study also showed that the effects of Hep and CAS were stronger than those of LMWH and MS871 at the same dose level. In view of the level of LDH, the four kinds of polysaccharide sulfate can protect cells from oxygen free radical injury. The effects of Hep and CAS were more active than those of LMWH.
Hu
M W
MW
Zhou
X B
XB
Zhang
L H
LH
Xu
H Y
HY
D002478:Cells, Cultured; D002809:Chondroitin Sulfates / Q000494:pharmacology*; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects*; D016166:Free Radical Scavengers / Q000494:pharmacology*; D006493:Heparin / Q000494:pharmacology*; D006495:Heparin, Low-Molecular-Weight / Q000494:pharmacology; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D014471:Umbilical Veins / Q000166:cytology / Q000187:drug effects*
D016166:Free Radical Scavengers; D006495:Heparin, Low-Molecular-Weight; D006493:Heparin; D002809:Chondroitin Sulfates; D007770:L-Lactate Dehydrogenase