The moss, Physcomitrella patens, transformed with apoaequorin cDNA responds to cold shock, mechanical perturbation and pH with transient increases in cytoplasmic calcium.
The gene for apoaequorin has been used previously to indicate cytosolic calcium changes in higher plants. Here we report the transformation of the moss Physcomitrella patens with the cDNA for apoaequorin. Stable transformants were obtained in the wild type which reconstitute the calcium-sensitive luminescent protein aequorin in vivo after incubation in coelenterazine, and continue to grow normally. The wild type responds to cold-shock (0-10 degrees C) with increases in cytosolic calcium. Mechanical perturbation, in the form of touch, also induces transient increases in cytosolic calcium. A smaller response to pH, distinct from the touch response and exhibiting different kinetics, can also be detected.
Russell
A J
AJ
Department of Applied Genetics, John Innes Centre, Norwich, UK.
Knight
M R
MR
Cove
D J
DJ
Knight
C D
CD
Trewavas
A J
AJ
Wang
T L
TL
D000331:Aequorin / Q000235:genetics* / Q000378:metabolism; D001059:Apoproteins / Q000235:genetics* / Q000378:metabolism; D019068:Bryopsida / Q000235:genetics* / Q000378:metabolism; D002118:Calcium / Q000378:metabolism*; D003080:Cold Temperature; D003593:Cytoplasm / Q000378:metabolism; D018076:DNA, Complementary / Q000235:genetics*; D006863:Hydrogen-Ion Concentration; D008163:Luminescent Measurements; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D013314:Stress, Mechanical; D014170:Transformation, Genetic
D001059:Apoproteins; D018076:DNA, Complementary; D011994:Recombinant Proteins; C060014:apoaequorin; D000331:Aequorin; D002118:Calcium