title: Isolation of a full-length cDNA clone for human GTP cyclohydrolase I type 1 from pheochromocytoma.
abstract: Although the existence of three different cDNA forms of human GTP cyclohydrolase I (GCH I) have been reported (Togari et al., 1992), the full-length sequence of any human GCH I cDNA involving poly (A) tail has not yet been documented. In the present study, we first isolated a full-length cDNA clone encoding human GCH I type 1 from human pheochromocytoma cDNA library. The length of the cDNA insert was 2,921 base pairs including poly (A) tail. RNA blot analysis showed a single mRNA species of 4.0 kb in human pheochromocytoma tissue.
authors.affiliation: Department of Pharmacology, Fujita Health University School of Medicine, Aichi, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Nomura
authors.forename: M
authors.initials: M
authors.lastname: Ohtsuki
authors.forename: S
authors.initials: S
authors.lastname: Matsui
authors.forename: C
authors.initials: C
authors.lastname: Sumi-Ichinose
authors.forename: H
authors.initials: H
authors.lastname: Nomura
authors.forename: Y
authors.initials: Y
authors.lastname: Hagino
authors.forename: K
authors.initials: K
authors.lastname: Iwase
authors.forename: H
authors.initials: H
authors.lastname: Ichinose
authors.forename: K
authors.initials: K
authors.lastname: Fujita
authors.forename: T
authors.initials: T
authors.lastname: Nagatsu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000302:isolation & purification*; D004273:DNA, Neoplasm / Q000302:isolation & purification*; D006136:GTP Cyclohydrolase / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D010673:Pheochromocytoma / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics
chemical_list: D018076:DNA, Complementary; D004273:DNA, Neoplasm; D006136:GTP Cyclohydrolase