title: Isolation of vasodilatory active flavonoids from the traditional remedy Satureja obovata.
abstract: In the course of screening natural products for smooth muscle relaxant properties, the effects of constituents of Satureja obovata var. obovata (Lamiaceae) on vascular reactivity experiments were studied. Bioassay-guided fractionation led to pure vasodilator compounds, identified by spectroscopic methods as the flavonoids: naringenin, eriodictyol, and luteolin. The relaxant effects induced by different extracts and fractions obtained on KCl (80 mM) and noradrenaline (NA) (10(-6) M) induced contractions on isolated rat aorta were selected as bioassay procedures. The sustained contraction induced by NA (10(-6) M) and K+ (80 mM) were relaxed by luteoline (98.7 +/- 0.7%; 40.30 +/- 2.4%), naringenin (12.41 +/- 1.8%; 3.05 +/- 0.3%), and eriodictyol (67.48 +/- 3.3%; 17.93 +/- 2.1%) each at the dose of 5 x 10(-5)M, respectively.
authors.affiliation: Departamento de Farmacología, Facultad de Farmacia, Universidad Complutense, Madrid, Spain.
authors.forename: V R
authors.initials: VR
authors.lastname: Sánchez de Rojas
authors.forename: B
authors.initials: B
authors.lastname: Somoza
authors.forename: T
authors.initials: T
authors.lastname: Ortega
authors.forename: A M
authors.initials: AM
authors.lastname: Villar
mesh_terms: D000818:Animals; D001011:Aorta / Q000187:drug effects / Q000502:physiology; D044950:Flavanones; D005419:Flavonoids / Q000302:isolation & purification* / Q000494:pharmacology; D066298:In Vitro Techniques; D047311:Luteolin; D009119:Muscle Contraction / Q000187:drug effects*; D009126:Muscle Relaxation / Q000187:drug effects; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000502:physiology*; D009638:Norepinephrine / Q000494:pharmacology; D008517:Phytotherapy; D010946:Plants, Medicinal; D051381:Rats; D013030:Spain; D014665:Vasodilator Agents / Q000302:isolation & purification* / Q000494:pharmacology
chemical_list: D044950:Flavanones; D005419:Flavonoids; D014665:Vasodilator Agents; C005273:naringenin; D047311:Luteolin; C007619:eriodictyol; D009638:Norepinephrine