title: Solution structure of bovine angiogenin by 1H nuclear magnetic resonance spectroscopy.
abstract: The three-dimensional structure of bovine angiogenin has been determined using two- and three-dimensional proton NMR spectroscopy. The solution structure is very close to that recently determined by X-ray diffraction analysis. This structure appears well defined, even if five loops and one helix exhibit greater flexibility. Analysis of the active site geometry confirms the position of the Glu-118 residue which obstructs the pyrimidine binding site. There is no experimental evidence of an unobstructed conformation of angiogenin in solution. In addition, it appears that the Glu-118 and Ser-119 residues and the cell receptor binding loop may play an important role in the differences of C-terminal fragment organization and ribonucleolytic activity observed between angiogenins and ribonuclease A.
authors.affiliation: Départment de Chimie-Synthèse Organique, URA 1308 du CNRS, Ecole Polytechnique, Palaiseau, France.
authors.forename: O
authors.initials: O
authors.lastname: Lequin
authors.forename: C
authors.initials: C
authors.lastname: Albaret
authors.forename: F
authors.initials: F
authors.lastname: Bontems
authors.forename: G
authors.initials: G
authors.lastname: Spik
authors.forename: J Y
authors.initials: JY
authors.lastname: Lallemand
mesh_terms: D043925:Angiogenesis Inducing Agents / Q000737:chemistry*; D000818:Animals; D001665:Binding Sites; D002417:Cattle; D003198:Computer Simulation; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry*; D012259:Ribonuclease, Pancreatic / Q000737:chemistry; D012996:Solutions
chemical_list: D043925:Angiogenesis Inducing Agents; D011506:Proteins; D012996:Solutions; C046366:angiogenin; D012259:Ribonuclease, Pancreatic