title: A recombinant antibody with the antigen-specific, major histocompatibility complex-restricted specificity of T cells.
abstract: Specific recognition of peptide/major histocompatibility complex (MHC) molecule complexes by the T-cell receptor is a key reaction in the specific immune response. Antibodies against peptide/MHC complexes would therefore be valuable tools in studying MHC function and T-cell recognition and might lead to novel approaches in immunotherapy. However, it has proven difficult to generate antibodies with the specificity of T cells by conventional hybridoma techniques. Here we report that the phage display technology is a feasible alternative to generate antibodies recognizing specific, predetermined peptide/MHC complexes.
authors.affiliation: Department of Biological Sciences, The Royal Danish School of Pharmacy, Copenhagen, Denmark.
authors.forename: P S
authors.initials: PS
authors.lastname: Andersen
authors.forename: A
authors.initials: A
authors.lastname: Stryhn
authors.forename: B E
authors.initials: BE
authors.lastname: Hansen
authors.forename: L
authors.initials: L
authors.lastname: Fugger
authors.forename: J
authors.initials: J
authors.lastname: Engberg
authors.forename: S
authors.initials: S
authors.lastname: Buus
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000918:Antibody Specificity; D001435:Bacteriophages / Q000235:genetics; D015723:Gene Library; D008285:Major Histocompatibility Complex; D051379:Mice; D008806:Mice, Inbred AKR; D008969:Molecular Sequence Data; D010455:Peptides / Q000276:immunology*; D011994:Recombinant Proteins / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D010455:Peptides; D011994:Recombinant Proteins