UV- and gamma-irradiation-induced DNA single-strand breaks and their repair in human blood granulocytes and lymphocytes.
Ionizing irradiation and UV-irradiation cause DNA damage. Ionizing irradiation induces single-strand breaks, much less abundantly double-strand breaks, alkali-labile sites, and various oxidized purines and pyrimidines. UV-irradiation, on the other hand, causes cyclobutane pyrimidine dimers, (6-4) photoproducts, and various monomeric base damages. The deposition of energy in DNA may result directly in single-strand breaks (predominant form after ionizing radiation), or the strand breaks may be generated during the repair process (predominant form after UV-irradiation). We investigated the formation and repair of DNA single-strand breaks in human blood granulocytes and lymphocytes by the single-cell gel electrophoresis or comet assay. The induction and repair of DNA lesions by gamma-irradiation was comparable in human blood granulocytes and lymphocytes. The finding is consistent with the expression of the pertinent base excision repair proteins in these cells. In contrast to gamma-irradiation, fewer single-strand breaks were observed immediately after UV-irradiation; the maximum number of breaks were seen when the cells were incubated for 30-60 min. After an incubation period of 150 min, a significant reduction of single-strand breaks was noted. It is conceivable that the first 30-60 min represented a period during which the incision-excision phase of nucleotide excision repair (NER) predominated. After that, strand joining was dominant, evidently representing the synthesis and ligation phase of NER. These results indicate that the approx. 30 different polypeptides required for complete NER are functional in these mature blood cells. This is the first demonstration of the expression of global NER in human granulocytes.
Lankinen
M H
MH
Department of Clinical Chemistry, Tampere University Hospital, Finland.
Vilpo
L M
LM
Vilpo
J A
JA
D000328:Adult; D004249:DNA Damage; D004260:DNA Repair; D004277:DNA, Single-Stranded / Q000032:analysis; D004307:Dose-Response Relationship, Radiation; D005260:Female; D005720:Gamma Rays; D006098:Granulocytes / Q000378:metabolism* / Q000528:radiation effects; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism* / Q000528:radiation effects; D008297:Male; D008875:Middle Aged; D014466:Ultraviolet Rays
D004277:DNA, Single-Stranded