Generation of novel killer hybridomas derived from proliferation-suppressed somatic cell hybrids between YACUT T cell lymphoma and normal lymphocytes activated in secondary mixed lymphocyte cultures.
Somatic cell hybridization between the YACUT T cell lymphoma cell line with normal lymphocytes activated in secondary mixed lymphocyte cultures (MLCs) consistently yielded IL-2-dependent CD4- CD8 alpha+ beta- Fc gamma RIII+ hybrids with cytotoxic function. The hybrids expressed T cell receptors other than that of YACUT origin, and fusion of the YACUT with a CD8 alpha+ beta+ Fc gamma RIII- T cell line also yielded hybrids with an unexpected CD8 alpha+ beta- Fc gamma RIII+ phenotype, which two observations strongly suggested that CD8+ T cells became the parental cell of the hybrids. Prolonged growth of the hybrids with IL-2 resulted in the generation of autonomously growing hybrids (hybridomas) without abrogating the cytotoxic function. The hybridomas exhibited MHC-unrestricted cytotoxicity in a Ca(2+)-dependent manner without prior stimulation and also mediated antibody-dependent cellular cytotoxicity. These results indicate that novel killer hybridomas can be produced following cell transformation of proliferation-suppressed cytotoxic YACUT x MLC cell hybrids. The killer hybridomas may be of value for analyzing recognition mechanisms and molecules involved in MHC-unrestricted cell-mediated cytotoxicity.
Kubota
K
K
Department of Microbiology, Kitasato University School of Medicine, Kanagawa, Japan.
Nakazato
K
K
Tamauchi
H
H
Sasahara
T
T
Katoh
H
H
D000818:Animals; D000920:Antibody-Dependent Cell Cytotoxicity; D015703:Antigens, CD / Q000032:analysis; D001483:Base Sequence; D002455:Cell Division / Q000276:immunology; D002459:Cell Fusion; D002461:Cell Line, Transformed; D003602:Cytotoxicity, Immunologic; D006822:Hybrid Cells / Q000145:classification / Q000166:cytology / Q000276:immunology*; D006825:Hybridomas / Q000276:immunology*; D016130:Immunophenotyping; D007694:Killer Cells, Natural / Q000276:immunology*; D007940:Leukemia L5178 / Q000276:immunology / Q000378:metabolism; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D016399:Lymphoma, T-Cell / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008811:Mice, Inbred DBA; D008969:Molecular Sequence Data; D011948:Receptors, Antigen, T-Cell / Q000096:biosynthesis; D017452:Receptors, IgG / Q000235:genetics; D014407:Tumor Cells, Cultured
D015703:Antigens, CD; D011948:Receptors, Antigen, T-Cell; D017452:Receptors, IgG