title: 99mTc-dextran-antibody conjugates. Labelling procedures.
abstract: Dextran forms stable chelates with 99mTc, a radionuclide with ideal properties for planar scintigraphic and tomographic imaging. This study investigates some of the factors of importance to the formation of 99mTc-dextran. The complex was used for the technetium labelling of a monoclonal antibody. Two radiolabelling methods were studied: direct dextran labelling with the reductant dissolved in HCI and labelling via a weak 'transfer' chelator (tartaric acid) with the reductant dissolved in ethanol. Different conditions during the labelling reaction were studied. Finally, dextran was coupled to a monoclonal anticytokeratin antibody and the conjugate was subsequently radiolabelled with 99mTc. Gel filtration (GFR) and thin layer chromatography (TLC) were compared as methods for estimation of the labelling efficiency. When using 10-500 microM of ligand, 5-100 microM SnCl2 with 10-500 MBq of technetium at pH 7 incubated for 10-15 min, the radiolabelling seemed optimal (70-75% labelling efficiency). It was found that 100 microM tartaric acid used as a weak intermediate chelator with SnCl2 dissolved in ethanol improved the reproducibility of the labelling. The labelling efficiency was not affected by either the presence of oxygen or the addition of an oxygen scavenger during the labelling incubation. In general, TLC showed higher labelling efficiencies than GFR, indicating inadequate separation of the different moieties.
authors.affiliation: Department of Oncology, University Hospital, Uppsala, Sweden.
authors.forename: M
authors.initials: M
authors.lastname: Márquez
authors.forename: J E
authors.initials: JE
authors.lastname: Westlin
authors.forename: S
authors.initials: S
authors.lastname: Nilsson
authors.forename: A R
authors.initials: AR
authors.lastname: Holmberg
mesh_terms: D000911:Antibodies, Monoclonal / Q000737:chemistry*; D002614:Chelating Agents / Q000737:chemistry; D002850:Chromatography, Gel; D002855:Chromatography, Thin Layer; D003911:Dextrans / Q000138:chemical synthesis / Q000737:chemistry*; D000431:Ethanol / Q000737:chemistry; D006851:Hydrochloric Acid / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007633:Keratins / Q000276:immunology; D008024:Ligands; D015609:Organotechnetium Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D010084:Oxidation-Reduction; D010100:Oxygen / Q000737:chemistry; D016719:Radioimmunodetection; D011877:Radionuclide Imaging; D015203:Reproducibility of Results; D013644:Tartrates / Q000737:chemistry; D013667:Technetium / Q000737:chemistry; D017971:Tin Compounds / Q000737:chemistry; D014056:Tomography, X-Ray
chemical_list: D000911:Antibodies, Monoclonal; D002614:Chelating Agents; D003911:Dextrans; D008024:Ligands; D015609:Organotechnetium Compounds; D013644:Tartrates; D017971:Tin Compounds; C034146:technetium Tc 99m dextran; C023599:stannous chloride; D000431:Ethanol; D007633:Keratins; D013667:Technetium; D006851:Hydrochloric Acid; D010100:Oxygen; C029768:tartaric acid