Adhesive mucous gel layer and mucus release as intestinal barrier in rats.
BACKGROUND
Although it has been reported that total parenteral nutrition induces an increased intestinal permeability and a decreased mucous gel layer covering the intestinal epithelium, the role of mucous gel on intestinal permeability has not been well understood. We examined the in vivo effects of N-acetyl cysteine (NAC) as mucolytic agent and colchicine as suppressant of the mucus production on the intestinal transmission of fluorescein isothiocyanate dextran 70,000 (FITC-dextran).


METHODS
Rats were divided into four groups. In each group, FITC-dextran (750 mg/kg) with or without NAC (3000 mg/kg) was injected into the small intestinal lumen 3 hours after intraperitoneal injection of saline or colchicine (Col, 10 mg/kg). Thirty minutes after injection of FITC-dextran, blood samples were taken from portal vein to analyze plasma fluorescein concentration by fluorescence spectrometry. Samples of small intestine were sectioned in a cryostat for fluorescence microscopy, and the identical sections were stained by periodic acid-Schiff reaction.


RESULTS
Plasma FITC-dextran level in NAC group was higher than that in control group (p < .01), that in Col + NAC group was higher than that in Col group (p < .01) and that in Col + NAC group was higher than that in NAC group (p < .05). The spaces between villi were filled with mucous gel in the control and Col groups, whereas those were not entirely filled with mucous gel in NAC and Col + NAC groups. FITC-dextran and mucous gel showed complementary distribution in all rats. The villous interstitial edema was recognized in NAC group and the villi were disrupted in Col + NAC group.


CONCLUSIONS
These results suggest that intestinal permeability is possibly affected not only by the mucous gel covering the intestinal epithelium but also by mucus release from goblet cells of the small intestine.
Iiboshi
Y
Y
Department of Pediatric Surgery, Osaka University Medical School, Japan.
Nezu
R
R
Cui
L
L
Chen
K
K
Khan
J
J
Yoshida
H
H
Sando
K
K
Kamata
S
S
Takagi
Y
Y
Okada
A
A
D000111:Acetylcysteine / Q000494:pharmacology*; D000268:Adhesiveness; D000818:Animals; D002463:Cell Membrane Permeability / Q000187:drug effects; D003078:Colchicine / Q000494:pharmacology*; D003911:Dextrans / Q000378:metabolism; D016650:Fluorescein-5-isothiocyanate / Q000031:analogs & derivatives / Q000378:metabolism; D005782:Gels; D007413:Intestinal Mucosa / Q000187:drug effects / Q000502:physiology*; D007421:Intestine, Small / Q000033:anatomy & histology / Q000502:physiology; D008297:Male; D008856:Microscopy, Fluorescence; D009093:Mucus / Q000378:metabolism*; D010503:Periodic Acid-Schiff Reaction; D051381:Rats; D017207:Rats, Sprague-Dawley
D003911:Dextrans; D005782:Gels; C015219:fluorescein isothiocyanate dextran; D016650:Fluorescein-5-isothiocyanate; D003078:Colchicine; D000111:Acetylcysteine