title: An experimental ovine foot abscess model using a Fusobacterium necrophorum biotype AB.
abstract: An experimental procedure is described for the production of foot abscess in sheep that mimics the natural disease. Lesions were produced by the intradermal inoculation of suspensions of Fusobacterium necrophorum biotype AB containing from 5 x 10(2) to 5 x 10(8) bacteria, into interdigital skin devitalized by freezing with liquid nitrogen. A dose of 5 x 10(5) bacteria induced the development of foot abscess in 3 of 4 and 8 of 8 inoculated feet. It was found that to produce foot abscess in devitalized tissue required between 10(3) and 10(6) fewer bacteria than were necessary to produce similar lesions in healthy tissue.
authors.affiliation: CSIRO, Animal Health Research Laboratory, Vic, Australia.
authors.forename: L A
authors.initials: LA
authors.lastname: Corner
authors.forename: N D
authors.initials: ND
authors.lastname: Collins
authors.forename: J A
authors.initials: JA
authors.lastname: Vaughan
mesh_terms: D000038:Abscess / Q000382:microbiology / Q000473:pathology / Q000662:veterinary*; D000818:Animals; D005533:Foot Dermatoses / Q000382:microbiology / Q000473:pathology / Q000662:veterinary*; D005674:Fusobacterium Infections / Q000382:microbiology / Q000473:pathology / Q000662:veterinary*; D005675:Fusobacterium necrophorum; D008297:Male; D012756:Sheep; D012757:Sheep Diseases / Q000382:microbiology / Q000473:pathology*