Lipid fluidity of triacylglycerol-rich lipoproteins isolated from copper-deficient rats.
Triacylglycerol-rich lipoproteins (TGRLP) were isolated from Cu-deficient and control rats. TGRLP from Cu-deficient rats appeared more fluid than those from controls as sensed by the fluorescence anisotropy of 1,6-diphenyl-1,3,5-hexatriene (DPH). This high fluidity was related to a low cholesterol:phospholipid ratio and high triacylglycerol content in these lipoproteins. TGRLP from Cu-deficient rats were more susceptible to in vitro peroxidation than lipoproteins from control rats as shown by the rate of diene conjugation. The damage induced by the peroxidation resulted in a more ordered state of the lipid fraction especially in lipoproteins from Cu-deficient rats. Thus, after in vitro peroxidation, TGRLP from Cu-deficient rats were more rigid than those from controls. These results suggest that Cu deficiency induces modifications in physicochemical properties of TGRLP which could affect their metabolism.
Motta
C
C
Centre de Recherche en Nutrition Humaine de Clermont-Ferrand, Hôtel Dieu, Clermont-Ferrand, France.
Gueux
E
E
Mazur
A
A
Rayssiguier
Y
Y
D000818:Animals; D003300:Copper / Q000008:administration & dosage* / Q000172:deficiency; D004161:Diphenylhexatriene; D066298:In Vitro Techniques; D050356:Lipid Metabolism; D008074:Lipoproteins / Q000097:blood* / Q000737:chemistry / Q000378:metabolism; D008297:Male; D010084:Oxidation-Reduction; D051381:Rats; D017208:Rats, Wistar; D014280:Triglycerides / Q000378:metabolism*
D008074:Lipoproteins; D014280:Triglycerides; D004161:Diphenylhexatriene; D003300:Copper