title: Determination of hepatitis A antibody response to vaccination by an enzyme immunoassay.
abstract: The quantitative responses to vaccination with hepatitis A vaccine was determined in 113 volunteers using a commercially available enzyme-linked immunosorbent assay for total antibodies to hepatitis A. Administration of vaccine or control preparation was carried out according to two regimens; at 0, 1 and 12 months (regimen I) and at 0, 0.5 and 12 months (regimen II). Seroconversion rates (concentrations of HAV antibodies > 50 IU/l) were between 94 and 97% at month 1 for regimen I and regimen II, respectively. The geometric mean titres (GMTs) fell gradually by month 12, and increased rapidly 10-100 fold 1 month after the booster dose at month 12. The GMTs of the groups receiving the control preparation remained below 50 IU/l. No significant differences were found between the antibody responses after regimen I or regimen II. It is concluded that the antibody test (Hepanostika HAV Antibody) can be used safely and adequately for quantitation of responses to hepatitis A immunisation.
authors.affiliation: Academic Unit of Travel Medicine and Vaccines, Royal Free Hospital School of Medicine, London, UK.
authors.forename: J N
authors.initials: JN
authors.lastname: Zuckerman
authors.forename: L
authors.initials: L
authors.lastname: Powell
authors.forename: R M
authors.initials: RM
authors.lastname: Lequin
authors.forename: A J
authors.initials: AJ
authors.lastname: Zuckerman
mesh_terms: D000328:Adult; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D035922:Hepatitis A Antibodies; D022362:Hepatitis A Vaccines; D006508:Hepatitis Antibodies / Q000097:blood*; D006507:Hepatovirus / Q000276:immunology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D011933:Reagent Kits, Diagnostic; D014611:Vaccination; D014761:Viral Hepatitis Vaccines / Q000276:immunology*
chemical_list: D035922:Hepatitis A Antibodies; D022362:Hepatitis A Vaccines; D006508:Hepatitis Antibodies; D011933:Reagent Kits, Diagnostic; D014761:Viral Hepatitis Vaccines