title: Growth and viability of Alcaligenes eutrophus JMP134 in seawater as affected by substrate and nutrient amendment.
abstract: Growth and viability of Alcaligenes eutrophus JMP134 was studied in laboratory microcosms with 0.2 microns-filtered seawater prior to release in field-based mesocosms. In unamended systems JMP134 did not grow and viability, measured as direct viable counts combined with immunofluorescence microscopy, was 40-50%. Addition of a nitrogen + phosphorus nutrient mixture caused a greater growth response than amendment with a carbon substrate. Amendment with substrate and/or nutrients caused an increase in viability to ca 100% but only for a brief period coinciding with cell proliferation. Hence, Alc. eutrophus JMP134 has a limited survival potential in seawater unless it is supplied with additional nutrients.
authors.affiliation: Department of Ecology and Molecular Biology, Royal Veterinary and Agricultural University, Frederiksberg C, Denmark.
authors.forename: O
authors.initials: O
authors.lastname: Nybroe
authors.forename: K
authors.initials: K
authors.lastname: Einarson
authors.forename: T
authors.initials: T
authors.lastname: Ahl
mesh_terms: D000421:Alcaligenes / Q000254:growth & development*; D015169:Colony Count, Microbial; D003470:Culture Media; D012623:Seawater / Q000382:microbiology*; D014871:Water Microbiology
chemical_list: D003470:Culture Media