title: Direct detection of Hb C (B6 Glu-Lys) by BseRI analysis.
abstract: Hemoglobin C (B6 Glu-Lys) can be identified by different techniques. Here we describe a restriction enzyme digestion protocol (BseRI GAGGAG N10) for direct detection of this variant.
authors.affiliation: Pediatric Molecular Pathology Department, Ankara University, Turkey.
authors.forename: N
authors.initials: N
authors.lastname: Akar
authors.forename: E
authors.initials: E
authors.lastname: Akar
authors.forename: H
authors.initials: H
authors.lastname: Taştan
authors.forename: S
authors.initials: S
authors.lastname: Cin
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism*; D004587:Electrophoresis, Agar Gel; D006580:Genetic Carrier Screening / Q000379:methods; D005914:Globins / Q000235:genetics; D006444:Hemoglobin C / Q000032:analysis* / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data
chemical_list: D005914:Globins; D004247:DNA; D006444:Hemoglobin C; C082523:endodeoxyribonuclease BseRI; D015252:Deoxyribonucleases, Type II Site-Specific