The unique organization of the rpoB region of Spiroplasma citri: a restriction and modification system gene is adjacent to rpoB.
A 6.5-kb DNA fragment containing the gene (rpoB) encoding the RNA polymerase (RNAP) beta subunit, from the mollicute Spiroplasma citri (Sc), was cloned and sequenced. The classical eubacterial organization, with the genes (rplK, A, J and L) encoding ribosomal proteins L11, L1, L10 and L12 located immediately upstream from rpoB, was not found in the Sc DNA. Instead, an open reading frame (hsdS) potentially encoding a component of a type I restriction and modification system was identified upstream from rpoB, and sequences showing similarities with insertion elements were found between hsdS and rpoB.
Laigret
F
F
Laboratoire de Biologie Cellulaire et Moléculaire, Institut National de la Recherche Agronomique et Université de Bordeaux II, Villenave d'Ornon, France. laigret@bordeaux.inra.fr
Gaurivaud
P
P
Bové
J M
JM
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D015280:DNA Restriction-Modification Enzymes / Q000235:genetics*; D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D015253:Deoxyribonucleases, Type I Site-Specific / Q000235:genetics; D005798:Genes, Bacterial / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013149:Spiroplasma / Q000201:enzymology / Q000235:genetics*
D015280:DNA Restriction-Modification Enzymes; D012321:DNA-Directed RNA Polymerases; D015253:Deoxyribonucleases, Type I Site-Specific