High-performance anion-exchange chromatography of oligonucleotides and oligodeoxynucleotides on quaternized polyethylenimine-coated zirconia.
The use of porous, quaternized polyethylenimine-coated zirconia particles for the separation of nucleosides, nucleotides, oligonucleotides, and oligodeoxynucleotides is described. Separations of these biomolecules on quaternized PEI-zirconia are presented. Quaternized PEI-zirconia and a quaternary amine functionalized silica are compared chromatographically. Quaternized PEI-zirconia was able to separate oligonucleotides and oligodeoxynucleotides differing in length by a single nucleotide unit. It could also separate RNAs of the same length but differing by only one deoxynucleotide. In contrast, the silica-based phase was unstable at elevated temperatures (75 degrees C), and all retention toward anions was lost quite rapidly.
McNeff
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Department of Chemistry, University of Minnesota, Minneapolis 55455, USA.
Carr
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PW
D001483:Base Sequence; D002852:Chromatography, Ion Exchange; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000302:isolation & purification*; D011094:Polyethyleneimine; D015040:Zirconium
D009841:Oligonucleotides; D011094:Polyethyleneimine; D015040:Zirconium; C028541:zirconium oxide