title: Adenovirus-mediated transfer of a wild-type p53 gene and induction of apoptosis in cervical cancer.
abstract: In most cervical cancers, the function of p53 is down regulated. To explore the potential use of p53 in gene therapy for cervical cancer, we introduced wild-type p53 into cervical cancer cell lines via a recombinant adenoviral vector, Ad5CMV-p53, and analyzed its effects on cell and tumor growth. The transduction efficiencies of all cell lines were 100% at a multiplicity of infection of 100 or greater. The p53 protein was detected in Ad5CMV-p53-infected cells. Protein expression peaked at day 3 after infection and lasted 15 days. The Ad5CMV-p53-infected cells underwent apoptosis, and cell growth was greatly suppressed. The Ad5CMV-p53 treatment significantly reduced the volumes of established s.c. tumors in vivo. These results indicate that transfection of cervical cancer cells with the wild-type p53 gene via Ad5CMV-p53 is a potential novel approach to the therapy of cervical cancer.
authors.affiliation: Section of Thoracic Molecular Oncology, Department of Thoracic and Cardiovascular Surgery, The University of Texas M.D. Anderson Cancer Center, Houston 77030, USA.
authors.forename: K
authors.initials: K
authors.lastname: Hamada
authors.forename: R
authors.initials: R
authors.lastname: Alemany
authors.forename: W W
authors.initials: WW
authors.lastname: Zhang
authors.forename: W N
authors.initials: WN
authors.lastname: Hittelman
authors.forename: R
authors.initials: R
authors.lastname: Lotan
authors.forename: J A
authors.initials: JA
authors.lastname: Roth
authors.forename: M F
authors.initials: MF
authors.lastname: Mitchell
mesh_terms: D000256:Adenoviridae / Q000235:genetics / Q000378:metabolism; D000258:Adenovirus Infections, Human / Q000235:genetics; D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D002455:Cell Division / Q000502:physiology; D005260:Female; D015870:Gene Expression; D016158:Genes, p53; D015316:Genetic Therapy; D005822:Genetic Vectors; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D014161:Transduction, Genetic; D014407:Tumor Cells, Cultured; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis; D002583:Uterine Cervical Neoplasms / Q000235:genetics / Q000628:therapy* / Q000821:virology
chemical_list: D016159:Tumor Suppressor Protein p53