Protoplast culture and plant regeneration from the suspension cells of Gynostemma pentaphyllum (Thumb) Mak.
From the suspension cultures of Gynostemma pentaphyllum (Thumb) Mak. protoplasts were isolated and cultured in a different medium with liquid and nurse culture method. Cell division was observed within 4 days and microcalli were formed within 4 weeks. On an MS solid medium with 1 mg/L of KT and 0.5 mg/L of IAA, protoplast-derived calli differentiated into embryos. Stems and leaves were formed on the MS medium with 1 mg/L of 6-BA and 0.5 mg/L of IAA. Finally, complete plantlets were obtained on a hormone-free MS solid medium.
Zhang
H
H
Cytogenetics Institute, Nanjing Agricultural University, China.
Wu
Q
Q
Liu
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D
D002478:Cells, Cultured / Q000166:cytology; D010946:Plants, Medicinal / Q000166:cytology / Q000254:growth & development*; D011523:Protoplasts / Q000166:cytology*; D012639:Seeds / Q000166:cytology / Q000254:growth & development