title: Evaluation of monoclonal antibodies with specificity for human IgA, IgA subclasses and allotypes and secretory component. Results of an IUIS/WHO collaborative study.
abstract: 51 monoclonal antibodies (McAb) with putative specificity for human IgA, the IgA subclasses, Am allotypes or secretory component (SC) were evaluated for immunoreactivity and specificity by nine laboratories employing immunodiffusion, agglutination, immunohistological assays, immunoblotting and direct binding and competitive inhibition enzyme immunoassays. McAbs specific for IgA PAN (n = 24), IgA1 (n = 7), IgA2 (n = 3), IgA2m(2) (n = 2), non-IgA2m(2) (n = 4) and SC or secretory IgA (n = 5) were identified that were immunoreactive and specific in the assays employed. The McAbs identified as IgA- or SC-reactive were shown to be non-reactive to human IgG, IgM, IgD, IgE, kappa and lambda by direct binding and competitive inhibition immunoassays. Interestingly, no McAbs with restricted specificity for IgA2m(1) were identified. Some McAbs displayed higher affinity and/or better performance in one or several of the assay groups. The IgA- and SC-specific McAbs identified in this international collaborative study have potential as immunochemical reference reagents to identify and quantitate monomeric and polymeric IgA in human serum and secretions.
authors.affiliation: University of Alabama, Birmingham, USA.
authors.forename: J
authors.initials: J
authors.lastname: Mestecky
authors.forename: R G
authors.initials: RG
authors.lastname: Hamilton
authors.forename: C G
authors.initials: CG
authors.lastname: Magnusson
authors.forename: R
authors.initials: R
authors.lastname: Jefferis
authors.forename: J P
authors.initials: JP
authors.lastname: Vaerman
authors.forename: M
authors.initials: M
authors.lastname: Goodall
authors.forename: G G
authors.initials: GG
authors.lastname: de Lange
authors.forename: I
authors.initials: I
authors.lastname: Moro
authors.forename: P
authors.initials: P
authors.lastname: Aucouturier
authors.forename: J
authors.initials: J
authors.lastname: Radl
authors.forename: C
authors.initials: C
authors.lastname: Cambiaso
authors.forename: C
authors.initials: C
authors.lastname: Silvain
authors.forename: J L
authors.initials: JL
authors.lastname: Preud'homme
authors.forename: K
authors.initials: K
authors.lastname: Kusama
authors.forename: G M
authors.initials: GM
authors.lastname: Carlone
authors.forename: J
authors.initials: J
authors.lastname: Biewenga
authors.forename: K
authors.initials: K
authors.lastname: Kobayashi
authors.forename: F
authors.initials: F
authors.lastname: Skvaril
authors.forename: C B
authors.initials: CB
authors.lastname: Reimer
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry* / Q000145:classification*; D000918:Antibody Specificity; D001666:Binding Sites, Antibody; D000939:Epitopes / Q000737:chemistry / Q000276:immunology; D006801:Humans; D007070:Immunoglobulin A / Q000737:chemistry / Q000145:classification* / Q000276:immunology*; D007071:Immunoglobulin A, Secretory / Q000276:immunology; D007126:Immunoglobulin Allotypes / Q000737:chemistry / Q000276:immunology*; D007158:Immunologic Techniques / Q000592:standards; D051379:Mice; D012015:Reference Standards; D012635:Secretory Component / Q000737:chemistry / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000939:Epitopes; D007070:Immunoglobulin A; D007071:Immunoglobulin A, Secretory; D007126:Immunoglobulin Allotypes; D012635:Secretory Component