Direct detection of Hb C (B6 Glu-Lys) by BseRI analysis.
Hemoglobin C (B6 Glu-Lys) can be identified by different techniques. Here we describe a restriction enzyme digestion protocol (BseRI GAGGAG N10) for direct detection of this variant.
Akar
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N
Pediatric Molecular Pathology Department, Ankara University, Turkey.
Akar
E
E
Taştan
H
H
Cin
S
S
D001483:Base Sequence; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism*; D004587:Electrophoresis, Agar Gel; D006580:Genetic Carrier Screening / Q000379:methods; D005914:Globins / Q000235:genetics; D006444:Hemoglobin C / Q000032:analysis* / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data
D005914:Globins; D004247:DNA; D006444:Hemoglobin C; C082523:endodeoxyribonuclease BseRI; D015252:Deoxyribonucleases, Type II Site-Specific