title: Prolonged intraocular pressure reduction following intravitreal barium injection in rabbits.
abstract: The isolated ciliary epithelium contains barium-inhibitable potassium channels. The present study was aimed at testing the in vivo effects of barium on aqueous humor dynamics in rabbits. BaCl2 was administered to one eye by topical delivery or intravitreal injection. Dynamic measurements included intraocular pressure, outflow facility, episcleral venous pressure and aqueous flow (fluorophotometry). Barium dynamics were studied using 133Ba. Intraocular pressure was not altered after topical administration of BaCl2. 133Ba was not detected in the aqueous after delivery of eyedrops containing the radiochemical. Intraocular pressure decreased following intravitreal injection of BaCl2 (0.15 microgram). The onset of this pressure reduction was 12 to 16 hr, the maximum decrease (-11.3 mmHg) occurred at 2 days, and the effect persisted (-4.2 mmHg) for approximately 12 days after the injection. Outflow facility and episcleral venous pressure were not altered. However, aqueous humor flow 5 to 6 days after the injection was decreased by 42% to 63% as determined by fluorophotometry or calculated tonographic data. The injection was not associated with findings of intraocular inflammation. Radioactivity was detected in the vitreous within the first 3 days after the injection; however, activity was present in the ciliary body, equally distributed between the cell membrane and soluble fractions, seven days after the injection. Intravitreally injected BaCl2 results in a prolonged intraocular pressure decrease relating to reduced aqueous formation. While the mechanism(s) for the BaCl2-induced decrease in pressure are not clear, possibilities include a Ba2+ interaction with ciliary epithelial K+ or N-type Ca2+ channels.
authors.affiliation: Department of Ophthalmology, Northwestern University Medical School, Chicago, IL 60611, USA.
authors.forename: T
authors.initials: T
authors.lastname: Krupin
authors.forename: O K
authors.initials: OK
authors.lastname: Park
authors.forename: A
authors.initials: A
authors.lastname: Hyderi
authors.forename: D
authors.initials: D
authors.lastname: Karalekas
authors.forename: M J
authors.initials: MJ
authors.lastname: Fritz
mesh_terms: D000818:Animals; D001082:Aqueous Humor / Q000187:drug effects; D017609:Barium Compounds / Q000494:pharmacology*; D001465:Barium Radioisotopes / Q000493:pharmacokinetics; D002712:Chlorides / Q000494:pharmacology*; D002924:Ciliary Body / Q000378:metabolism; D007429:Intraocular Pressure / Q000187:drug effects*; D007498:Iris / Q000378:metabolism; D011817:Rabbits; D012160:Retina / Q000378:metabolism; D013997:Time Factors; D014822:Vitreous Body / Q000378:metabolism
chemical_list: D017609:Barium Compounds; D001465:Barium Radioisotopes; D002712:Chlorides; C024986:barium chloride