Maltokinase (ATP:maltose 1-phosphotransferase) from Actinoplanes sp.: demonstration of enzyme activity and characterization of the reaction product.
Cell free extract of the acarbose producer Actinoplanes sp. catalyzes ATP-dependent phosphorylation of maltose. This was shown by two different assays. The product was purified and its structure determined to be alpha-maltose-1-phosphate by chemical analysis and NMR spectroscopy.
Drepper
A
A
Institut für Mikrobiologie, Westfälische Wilhelms-Universität, Münster, Germany.
Peitzmann
R
R
Pape
H
H
D000192:Actinomycetales / Q000201:enzymology*; D000255:Adenosine Triphosphate / Q000378:metabolism*; D001665:Binding Sites; D009682:Magnetic Resonance Spectroscopy; D008320:Maltose / Q000378:metabolism*; D009249:NADP / Q000378:metabolism; D010766:Phosphorylation; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000378:metabolism*; D013403:Sugar Phosphates / Q000378:metabolism
D013403:Sugar Phosphates; C080751:maltose 1-phosphate; D009249:NADP; D008320:Maltose; D000255:Adenosine Triphosphate; D017853:Phosphotransferases (Alcohol Group Acceptor); C100489:maltokinase