Identification and cDNA cloning of two novel low molecular weight host-protective antigens from Taenia ovis oncospheres.
Oncosphere antigens of Taenia ovis were solubilised in sodium dodecyl sulphate and separated by electrophoresis in polyacrylamide gels (SDS-PAGE). Antigen-containing gel fractions cut from the region covering 18-12 kDa were shown to be highly immunogenic in sheep challenge experiments. Specific antisera against 2 candidate antigens at 18 and 16 kDa were used to screen a cDNA library prepared from T. ovis oncosphere mRNA. Recombinant proteins selected with antibody to the 16 and 18 kDa native antigens were expressed as GST fusion proteins. Vaccination trials using either of the 2 fusion proteins To16.17-GST and To18-GST, revealed that each was capable of inducing high levels of immunity in sheep against challenge infection with T. ovis eggs. Antibodies induced by vaccination with the recombinant antigens reacted specifically with their respective 18 or 16 kDa native oncosphere antigens. There was no apparent homology between the T. ovis cDNA coding for To18 and To16.17, or with another host-protective antigen, To45W, described previously. These additional host-protective antigens should prove a valuable adjunct to To45W and permit the development of effective vaccination strategies.
Harrison
G B
GB
Mallinckrodt Veterinary New Zealand Ltd, Development Department, Upper Hutt, New Zealand.
Heath
D D
DD
Dempster
R P
RP
Gauci
C
C
Newton
S E
SE
Cameron
W G
WG
Robinson
C M
CM
Lawrence
S B
SB
Lightowlers
M W
MW
Rickard
M D
MD
D000595:Amino Acid Sequence; D000818:Animals; D000947:Antigens, Helminth / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification*; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D018509:DNA, Helminth / Q000235:genetics*; D015801:Helminth Proteins / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D012756:Sheep; D012757:Sheep Diseases / Q000276:immunology / Q000517:prevention & control; D013621:Taenia / Q000235:genetics* / Q000276:immunology*; D013622:Taeniasis / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary; D014611:Vaccination / Q000662:veterinary
D000947:Antigens, Helminth; D018076:DNA, Complementary; D018509:DNA, Helminth; D015801:Helminth Proteins; D011993:Recombinant Fusion Proteins