Cadmium ions influence the chemiluminescence activity of murine splenocytes both in vitro and in vivo.
The luminol-enhanced chemiluminescence (CL) activity of splenocytes of mice of the strain (CBA x C57B1)F1 was monitored after treatment with Cd2+ (cadmium chloride) in both in vitro and in vivo experiments. Cd2+ (at concentrations of 1 microM-1 mM) increased the CL reaction of the splenocytes (2 x 10(6) cells/ml) in vitro in a dose-dependent manner, both instantaneously and after incubation for 1 h. In in vivo experiments, Cd2+ was administered in two ways. Following a 14-day administration of cadmium to mice in drinking water (300 mg Cd2+/l), the CL reaction of the splenocytes was significantly reduced. On the other hand, after i.p. administration of CdCl2 dissolved in PBS (2 mg/kg body mass, repeated seven times during 14 days), the metabolic activity of the phagocytes was increased. From the results it follows that cadmium affects the immune system. However, its toxicity is dependent on the route of administration.
Cíz
M
M
Institute of Biophysics, Academy of Sciences of the Czech Republic, Brno, Czech Republic.
Lojek
A
A
Hosek
B
B
Feilhauerová
M
M
D000284:Administration, Oral; D000818:Animals; D002104:Cadmium / Q000008:administration & dosage / Q000633:toxicity*; D019256:Cadmium Chloride; D002273:Carcinogens / Q000633:toxicity; D002712:Chlorides / Q000008:administration & dosage / Q000633:toxicity; D004305:Dose-Response Relationship, Drug; D004326:Drinking; D066298:In Vitro Techniques; D007274:Injections, Intraperitoneal; D007700:Kinetics; D008163:Luminescent Measurements; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D010586:Phagocytes / Q000187:drug effects*; D010587:Phagocytosis / Q000187:drug effects; D013154:Spleen / Q000166:cytology / Q000187:drug effects* / Q000276:immunology
D002273:Carcinogens; D002712:Chlorides; D002104:Cadmium; D019256:Cadmium Chloride