Functional difference between Thy-1-positive and Thy-1-negative gamma delta T cells induced by Escherichia coli infection in mice.
There is an increase in number of gamma delta T cells in the peritoneal cavity after intraperitoneal (i.p.) inoculation with Escherichia coli. The E. coli-induced gamma delta T cells in C3H/He mice contain a large amount of Thy-1-negative population in addition to the Thy-1-positive population. We investigated the difference between the Thy-1-positive and the Thy-1-negative gamma delta T cells. Although it was found that only up to 4% of the gamma delta T cells on day 5 after infection were in cycling phase, and that the gamma delta T cells did not proliferate by immobilized anti-T-cell receptor (TCR) gamma delta monoclonal antibody stimulation, the gamma delta T cells proliferated in the presence of interleukin-2 (IL-2) and IL-7. The Thy-1-negative gamma delta T cells showed higher proliferative response compared with the Thy-1-positive gamma delta T cells. Furthermore, the Thy-1-negative gamma delta T cells showed lower IFN-gamma mRNA expression than the Thy-1-positive gamma delta T cells. On the other hand, both the Thy-1-positive and Thy-1-negative gamma delta T cells predominantly expressed V gamma 1, V gamma 4, V gamma 5, V gamma 6 and V delta 1, and no difference of V region usage was detected between them. These results suggest that functions of Thy-1-positive gamma delta T cells differ from Thy-1-negative gamma delta T cells although Thy-1-positive and Thy-1-negative gamma delta T cells may have similar V region repertoire and, possibly, similar antigen specificity.
Takada
H
H
Department of Immunology, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan.
Matsuzaki
G
G
Yoshida
H
H
Sumichika
H
H
Nomoto
K
K
D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage; D001483:Base Sequence; D002455:Cell Division / Q000276:immunology; D016207:Cytokines / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D004927:Escherichia coli Infections / Q000276:immunology*; D005260:Female; D007375:Interleukin-1 / Q000494:pharmacology; D007376:Interleukin-2 / Q000494:pharmacology; D051379:Mice; D008809:Mice, Inbred C3H; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000276:immunology*; D018417:Th1 Cells / Q000166:cytology / Q000276:immunology*
D000911:Antibodies, Monoclonal; D016207:Cytokines; D017931:DNA Primers; D007375:Interleukin-1; D007376:Interleukin-2; D012333:RNA, Messenger; D016692:Receptors, Antigen, T-Cell, gamma-delta