[Preparation and study of properties of anti-idiotypic antibodies, carrying hemagglutinating paratopes of tick-borne encephalitis virus on their surface].
Rabbit polyclonal and rat monoclonal anti-idiotypical antibodies (AIA) against two hemagglutinating murine monoclonal antibodies to glycoprotein E of tick-borne encephalitis virus (TBE) have been obtained, purified, and studied. All AIA reacted with murine monoclonal antibodies to TBE protein E and did not react with normal murine immunoglobulins in enzyme immunoassay (EIA). Polyclonal AIA to monoclonal E6B and 10H10 antibodies specifically reacted with RH, SPEV, and goose red cells. In addition, polyclonal AIA were capable of agglutinating goose erythrocytes. Mice immunized with purified polyclonal and monoclonal AIA produced antiviral sera, which reacted in EIA with purified TBE virions. These results indicate that AIA carry the TBE virus image and mimic the hemagglutination activity epitopes on glycoprotein E. Interactions of AIA with cell membranes indicates that they fix the cellular receptor needed for adsorption and penetration of TBE virus into the cells.
Protopopova
E V
EV
Khusainova
A D
AD
Konovalova
S N
SN
Loktev
V B
VB
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000096:biosynthesis* / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology; D000954:Antigens, Surface / Q000276:immunology*; D004669:Encephalitis Viruses, Tick-Borne / Q000276:immunology* / Q000502:physiology; D000939:Epitopes / Q000276:immunology; D005260:Female; D006386:Hemagglutination Tests; D006825:Hybridomas; D008561:Membrane Fusion; D051379:Mice; D051381:Rats; D014407:Tumor Cells, Cultured; D014759:Viral Envelope Proteins / Q000276:immunology
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D000939:Epitopes; D014759:Viral Envelope Proteins; C065696:glycoprotein E, Flavivirus
Poluchenie i izuchenie svoĭstv antiidiotipicheskikh antitel, nesushchikh na svoeĭ poverkhnosti gemaggliutiniruiushchie paratopy virusa kleshchevogo éntsefalita.