A new biochemical assay in the diagnostic management of nasal cerebrospinal fluid leakage.
A new method for the detection of cerebrospinal fluid (CSF) leakage is described, and is a refinement of the method originally reported by Oberascher and Arrer in 1986. Immuno-electrophoretic measurements are performed in a two-buffer system, making the test easier to do and providing qualitatively better images. Genetic variants of transferrin (which has a general population incidence of 2-4%) can be discriminated from false-positive test results in affected families. The test described is recommended as the method of choice for initial screening of suspected CSF leakage.
Middelweerd
M J
MJ
Department of Otorhinolaryngology/Head & Neck Surgery, Free University Hospital, Amsterdam, The Netherlands.
de Vries
N
N
Calliauw
J
J
van Kamp
G J
GJ
D000328:Adult; D000368:Aged; D001888:Boric Acids; D002021:Buffers; D002559:Cerebrospinal Fluid Rhinorrhea / Q000175:diagnosis* / Q000628:therapy*; D002951:Citrates; D019343:Citric Acid; D004587:Electrophoresis, Agar Gel; D005260:Female; D015522:Frontal Sinusitis / Q000601:surgery; D014644:Genetic Variation; D006801:Humans; D007122:Immunoelectrophoresis / Q000379:methods*; D007202:Indicators and Reagents; D018020:Lithium Compounds; D008297:Male; D010254:Paranasal Sinus Diseases / Q000150:complications; D011183:Postoperative Complications; D013101:Sphenoid Sinus / Q000473:pathology; D014168:Transferrin / Q000032:analysis / Q000235:genetics; D014325:Tromethamine
D001888:Boric Acids; D002021:Buffers; D002951:Citrates; D007202:Indicators and Reagents; D018020:Lithium Compounds; D014168:Transferrin; D014325:Tromethamine; D019343:Citric Acid; C028467:lithium hydroxide; C032688:boric acid