[The relationship between 4-hydroxynonenal-derived epitopes on apolipoprotein B and coronary heart disease].
When lipid peroxidation takes place in plasma low density lipoprotein (LDL), a lot of aldehydes-derived epitopes are generated. To explore the relationship between these aldehydes-derived epitopes on apolipoprotein B (apo B) and coronary heart disease (CHD), we used both antibody against 4-hydroxynonenal (HNE)-derived epitopes and antibody against apo B to establish a sandwich enzyme-linked immunoassay (ELISA). The sera from 160 normal controls as well as from 103 patients with CHD were tested by ELISA for the expression of HNE-derived epitopes on apo B. The measurements showed that the mean expression of HNE-epitopes in the patients with CHD (183.5 +/- 63.6 mg/L, n = 103) was higher than that of normal controls (133.3 +/- 47.5 mg/L, n = 160). The difference of the expression of HNE-epitopes on serum apo B between the patients and normal controls was statistically significant. The results analysed by a multiple regression demonstrated that the expression of HNE-epitopes, levels of LDL-cholesterol and age were positively related to CHD, while the levels of HDL-cholesterol and female were negatively related to CHD. Thus, it was proven for the first time that the enhanced expression of HNE-epitopes on apo B might be an independent risk factor of CHD.
Chen
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Atherosclerosis Research Center, Nanjing Medical University.
Cai
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Chen
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D000447:Aldehydes / Q000276:immunology*; D001055:Apolipoproteins B / Q000276:immunology*; D008078:Cholesterol, LDL / Q000097:blood; D003327:Coronary Disease / Q000276:immunology*; D005260:Female; D006801:Humans; D016056:Immunodominant Epitopes / Q000276:immunology*; D008297:Male; D008875:Middle Aged
D000447:Aldehydes; D001055:Apolipoproteins B; D008078:Cholesterol, LDL; D016056:Immunodominant Epitopes; C027576:4-hydroxy-2-nonenal