title: A clonally distinct recurrence of Burkitt's lymphoma at 15 years.
abstract: A human immunodeficiency virus-negative male was successfully treated for two occurrences of Burkitt's lymphoma, 15 years apart. As consolidation of his second remission, he underwent high-dose chemotherapy with peripheral blood stem cell transplantation. In an effort to prove whether the second lymphoma was a relapse of the first or a second primary lymphoma, we obtained paraffin-embedded material from both lymphomas. DNA was extracted from this material and amplified by polymerase chain reaction (PCR) using consensus JH and VH region primers. Analysis of the PCR products, which mostly reflects VDJ joints, showed two sharp bands of different molecular size, proving the monoclonal nature of the lymphomas and suggesting that each had different Ig gene rearrangements. Sequencing of both PCR products showed a marked dissimilarity in nucleotide sequence in the clonally unique VDJ joint region, providing strong evidence for the separate cellular genesis of each lymphoma. These results suggest that late relapses of Burkitt's lymphoma should be examined for clonal distinctiveness. If the second lymphoma is distinct from the primary one, it might be treated as a primary lymphoma rather than as recurrent disease.
authors.affiliation: Department of Medicine, University of Pittsburgh School of Medicine, PA 15213-2582, USA.
authors.forename: J
authors.initials: J
authors.lastname: Lister
authors.forename: J A
authors.initials: JA
authors.lastname: Miklos
authors.forename: S H
authors.initials: SH
authors.lastname: Swerdlow
authors.forename: D W
authors.initials: DW
authors.lastname: Bahler
mesh_terms: D000328:Adult; D001483:Base Sequence; D002051:Burkitt Lymphoma / Q000235:genetics / Q000473:pathology*; D002999:Clone Cells; D004273:DNA, Neoplasm / Q000235:genetics; D015326:Gene Rearrangement, B-Lymphocyte, Heavy Chain; D005803:Genes, Immunoglobulin; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D012008:Recurrence; D013997:Time Factors
chemical_list: D004273:DNA, Neoplasm