A microwave-mediated saponification of galactosylceramide and galactosylceramide I3-sulfate and identification of their lyso-compounds by delayed extraction matrix-assisted laser desorption ionization time-of-flight mass spectrometry.
Small amounts of galactosylceramide (cerebroside) and galactosylceramide I3-sulfate (sulfatide) obtained from porcine spinal cord and equine kidney were deacylated by a rapid method of microwave-mediated saponification to prepare their lyso-compounds. Mass spectra of their protonated or deprotonated molecular ion peaks were detected by recently developed new technology of a delayed extraction matrix-assisted laser desorption ionization time-of-flight mass spectrometer with reflector detector in positive or negative ion mode. Long chain bases of lysocerebroside and lysosulfatide were different between porcine spinal cord and equine kidney, but similar to each other in the same organ, suggesting their common synthetic pathway. It is noted that the new rapid method can be similarly applied to the deacylation of both cerebroside and sulfatide in contrast to our classical method which was able to be applied to cerebroside, but not to sulfatide.
Taketomi
T
T
Department of Biochemistry, Research Center on Aging and Adaptation, Shinshu University School of Medicine, Matsumoto, Japan.
Hara
A
A
Uemura
K
K
Kurahashi
H
H
Sugiyama
E
E
D000818:Animals; D005699:Galactosylceramides / Q000737:chemistry / Q000302:isolation & purification / Q000528:radiation effects*; D006736:Horses; D007668:Kidney / Q000737:chemistry; D008872:Microwaves; D011609:Psychosine / Q000737:chemistry / Q000302:isolation & purification; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D013116:Spinal Cord / Q000737:chemistry; D013433:Sulfoglycosphingolipids / Q000737:chemistry / Q000302:isolation & purification / Q000528:radiation effects*; D013552:Swine
D005699:Galactosylceramides; D013433:Sulfoglycosphingolipids; D011609:Psychosine