title: Immunobiology of methicillin-resistant Staphylococcus aureus: immune response of rabbits and patients to systemic infection.
abstract: Teichoic acid (TA) and peptidoglycan (PG) extracted from Staphylococcus aureus strains ATCC 25923 and Lafferty as well as formalinized cells of these two strains and several clinical methicillin-resistant S. aureus (MRSA) isolates were immunogenic for New Zealand White rabbits. Rabbits which had recovered from experimental bacteremia due to MRSA seroconverted, i.e. demonstrated raised titers of antibodies against TA and PG of the S. aureus strain Lafferty and against whole cells (WC) and ultrasound cell lysates (UCL) of MRSA isolates No. 1 and 2 (representative of nosocomial MRSA strain I), as determined with enzyme-linked immunosorbent assays. Furthermore, sera from 2 long-term survivor rabbits recognized four polypeptides (apparent molecular weight = 38.9, 33.9, 30.9, and 28.2 kDa) shared by UCL extracts from MRSA isolates No. 1 and 2, as determined with immunoblots. Neither normal nor immune rabbit sera augmented the bactericidal activity of fresh defibrinated human blood (65% v/v) against selected MRSA isolates and S. aureus strain ATCC 25923. Sera from 12 patients with documented systemic infection due to MRSA outbreak strain I were examined for IgM and IgG antibodies against TA, WC, and UCL antigens. Three patient sera exhibited raised IgM antibodies against TA; 7 of 12 patient sera showed increased IgG anti-TA titers. Only 1 patient had a markedly raised IgM anti-WC titer, whereas 4 and 10 of the patients had increased IgG titers against WC from MRSA isolates No. 1 and 2, respectively. However, all 12 patients had raised IgG titers against UCL from MRSA isolate No.2 versus 4 of 12 patients with elevated IgG titers against UCL from MRSA isolate No.1. Immunoblots with 3 selected patient sera revealed IgG antibodies to be more multifaceted than IgM antibodies. Sera from 11 of the 12 patients contained antimicrobial drug(s); yet only 5 of these 11 sera (used at 10% v/v in broth) killed inocula of MRSA isolate No. 43. None of the 12 patient sera (10% v/v) enhanced the bactericidal activity of human blood against selected MRSA isolates. Neither three commercial intravenously applicable IgG preparations nor an IgG anti-alpha-hemolysin formulation (employed at 10% v/v) augmented the bactericidal activity of fresh defibrinated human blood against selected MRSA isolates comprising MRSA outbreak strain I.
authors.affiliation: Institut für Medizinische Mikrobiologie und Hygiene, Universität des Saarlandes, Homburg/Saar, Germany.
authors.forename: W H
authors.initials: WH
authors.lastname: Traub
authors.forename: D
authors.initials: D
authors.lastname: Bauer
authors.forename: B
authors.initials: B
authors.lastname: Leonhard
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007075:Immunoglobulin M / Q000097:blood; D016106:Methicillin Resistance; D011817:Rabbits; D013203:Staphylococcal Infections / Q000276:immunology*; D013211:Staphylococcus aureus / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D007075:Immunoglobulin M