title: Mosaic structures of neurotoxins produced from Clostridium botulinum types C and D organisms.
abstract: We isolated the gene encoding a botulinum neurotoxin (BoNT) of 1285 amino acids with a molecular weight of 147,364 from the toxigenic bacteriophage d-sA of Clostridium botulinum type D strain South African (Dsa). The BoNT of Dsa (BoNT/Dsa) is composed of three regions on the basis of the homology to BoNT types C1 (BoNT/C1) and D (BoNT/D). The N-terminal (Met-1 to Val-522) and the C-terminal regions (Trp-945 to Glu-1285) have high identity to corresponding regions of BoNT/D (96% identity) and BoNT/C1 (74% identity), respectively. The core region (Pro-523 to Lys-944) is common to three toxins (83% to 92% identity). These results suggest that neurotoxins produced from Clostridium botulinum types C and D are composed in a mosaic-like fashion.
authors.affiliation: Department of Veterinary Science, National Institute of Health, Tokyo, Japan. kohji@nih.go.jp
authors.forename: K
authors.initials: K
authors.lastname: Moriishi
authors.forename: M
authors.initials: M
authors.lastname: Koura
authors.forename: N
authors.initials: N
authors.lastname: Abe
authors.forename: N
authors.initials: N
authors.lastname: Fujii
authors.forename: Y
authors.initials: Y
authors.lastname: Fujinaga
authors.forename: K
authors.initials: K
authors.lastname: Inoue
authors.forename: K
authors.initials: K
authors.lastname: Ogumad
mesh_terms: D000595:Amino Acid Sequence; D001905:Botulinum Toxins / Q000235:genetics*; D003001:Cloning, Molecular; D003014:Clostridium botulinum / Q000378:metabolism*; D008969:Molecular Sequence Data; D009030:Mosaicism; D017386:Sequence Homology, Amino Acid
chemical_list: D001905:Botulinum Toxins