Precolumn fluorescence derivatization of the antagonist [Arg6,D-Trp7,9,MePhe8]-substance P[6-11] with benzoin in high-performance liquid chromatography and selective detection of arginine-containing fragments in its degradation products.
Precolumn fluorescence derivatization for the determination of the antagonist [Arg6,D-Trp7,9,MePhe8]-Substance P¿6-11¿ (antagonist G) using benzoin in HPLC was studied. Under the conditions chosen (0.067 M NaOH, heating at 100 degrees C for 10 s), a good yield of fluorescent derivatives was obtained and no methodology-related degradation occurred. The detection limit of antagonist G was 0.21 nmol/ml. The method has been applied to the selective and sensitive detection of arginine-containing fragments in degradation products of antagonist G.
Cui
H
H
Department of Pharmaceutical Analysis, Faculty of Pharmacy, Utrecht University, Netherlands.
Reubsaet
J L
JL
Bult
A
A
D000595:Amino Acid Sequence; D001120:Arginine / Q000737:chemistry*; D001573:Benzoin / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D005456:Fluorescent Dyes / Q000737:chemistry; D007202:Indicators and Reagents / Q000737:chemistry; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000032:analysis* / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013373:Substance P / Q000031:analogs & derivatives* / Q000737:chemistry
D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D010446:Peptide Fragments; C046958:substance P (6-11), Arg(6)-Trp(7,9)-Me-Phe(8)-; D013373:Substance P; D001120:Arginine; D001573:Benzoin