Selective loss of myelin-associated glycoprotein from myelin correlates with anti-MAG antibody titre in demyelinating paraproteinaemic polyneuropathy.
The IgM monoclonal autoantibodies of patients with demyelinating paraproteinaemic polyneuropathy recognize a carbohydrate structure present on both myelin-associated glycoprotein (MAG) and protein zero (P0). These autoantibodies are sufficient to cause the disease but the mechanism of demyelination remains unclear. We have analysed nerve biopsies from eight patients with polyneuropathy and anti-MAG antibodies by quantitative immunohistochemistry and find a concordant pattern of reduced expression of myelin markers with the loss of myelinated fibres. We report here novel features of this disease, in particular a selective lack of detectable MAG in a large proportion of myelinated-fibres containing P0, myelin basic protein (MBP) and periaxin. There is also an inverse correlation of the distribution of MAG in peripheral nerve myelin with the serum anti-MAG antibody titres but no correlation of these titres with the loss of myelinated fibres. Double immunofluorescence staining of paraproteinaemic polyneuropathy (PPN) nerves shows anti-MAG IgM deposited on the periphery of myelinated fibres associated with or lacking MAG staining. These data suggest that the binding of anti-MAG antibodies to MAG and/or other myelin component(s) results in MAG downregulation and may have an essential role in the molecular mechanisms leading to demyelination and partial regeneration in this disease.
Gabriel
J M
JM
Department of Research, University Hospital Basel, Switzerland.
Erne
B
B
Miescher
G C
GC
Miller
S L
SL
Vital
A
A
Vital
C
C
Steck
A J
AJ
D000918:Antibody Specificity; D001323:Autoantibodies / Q000097:blood*; D015415:Biomarkers; D001706:Biopsy; D018959:CD57 Antigens / Q000032:analysis / Q000378:metabolism; D003711:Demyelinating Diseases / Q000276:immunology*; D005260:Female; D005455:Fluorescent Antibody Technique; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis / Q000378:metabolism; D006801:Humans; D007075:Immunoglobulin M / Q000097:blood; D007150:Immunohistochemistry; D008297:Male; D009185:Myelin Proteins / Q000737:chemistry / Q000378:metabolism; D009186:Myelin Sheath / Q000737:chemistry / Q000378:metabolism; D018992:Myelin-Associated Glycoprotein / Q000276:immunology*; D010265:Paraproteinemias / Q000276:immunology*; D010523:Peripheral Nervous System Diseases / Q000276:immunology*; D010543:Peroneal Nerve / Q000737:chemistry; D013194:Staining and Labeling
D001323:Autoantibodies; D015415:Biomarkers; D018959:CD57 Antigens; D005904:Glial Fibrillary Acidic Protein; D007075:Immunoglobulin M; D009185:Myelin Proteins; D018992:Myelin-Associated Glycoprotein