[Analysis of carbapenem resistant plasmid isolated from Serratia marcescens].
Four nontransferable plasmids of about 28-32 kbp were isolated in 120 Serratia marcescens strains isolated from patients with urinary tract infection. All of these plasmids were tranformable in Escherichia coli, and showed a high frequency of mobilization by R9-5 plasmid of incompatibility group F II. The MICs of carbapenems were significantly lower than those of cephalosporins. This trend was more marked when MICs were determined at 10(6)cfu/ml. Based on the substrate profiles and on the effect of inhibitors of the beta-lactamase, the enzyme was classified as a class B beta-lactamase, so called carbapenemase. The carbapenem resistance genes of these 4 plasmids were cloned into vector plasmid pHSG398 using Bam H1 digestion and the size of the DNA was found to be 4.9 kb.
Yosue
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Yosue Medical Clinic for Stomack and Intestinal Illness, Fukuoka.
D001426:Bacterial Proteins; D015780:Carbapenems / Q000494:pharmacology*; D010957:Plasmids; D012706:Serratia marcescens / Q000187:drug effects* / Q000235:genetics*; D018440:beta-Lactam Resistance; D001618:beta-Lactamases / Q000302:isolation & purification
D001426:Bacterial Proteins; D015780:Carbapenems; D001618:beta-Lactamases; C063912:carbapenemase