Nerve coaptation studies with and without a gap in rabbits.
Appropriate matching of proximal and distal fibers is a major objective when suturing a lacerated nerve. Recent studies suggest that neurotropic factors may influence motor/sensory specificity and affect the functional outcome. This was studied in animal models by direct coaptation of cut nerve ends inside a 5-mm collagen tube with and without appropriate sensory/motor alignment, as well as in models where the cut nerve ends were placed in a 10-mm collagen tube with a 5-mm gap, with and without appropriate sensory/motor alignment. The radial nerve of 49 New Zealand white rabbits was chosen because it has distinct motor and sensory divisions. The animals were killed at 24 weeks and electrophysiologic, histologic, and muscle contraction studies were performed. Axon counts and diameters were measured from the distal motor and sensory stumps. Nerve conduction velocity, dry muscle weight, and motor axon counts were not statistically different between the groups. The malaligned group without a gap had better regeneration in sensory nerves than other groups. The muscle contraction force of the malaligned group without a gap was significantly less than the other groups. The malaligned group with a 5-mm gap had the same muscle contraction force as the aligned group without a gap. In this study, a short nerve gap lessened the misdirection of motor fibers after nerve-end coaptation.
Hasegawa
J
J
Department of Orthopaedic Surgery, Niigata University School of Medicine, Japan.
Shibata
M
M
Takahashi
H
H
D000714:Anastomosis, Surgical / Q000379:methods*; D000818:Animals; D001369:Axons / Q000502:physiology; D001684:Biological Dressings; D008866:Microsurgery / Q000379:methods*; D009046:Motor Neurons / Q000502:physiology; D009119:Muscle Contraction / Q000502:physiology; D009413:Nerve Fibers, Myelinated / Q000502:physiology; D009416:Nerve Regeneration / Q000502:physiology; D009431:Neural Conduction / Q000502:physiology; D059348:Peripheral Nerve Injuries; D010525:Peripheral Nerves / Q000503:physiopathology / Q000601:surgery; D011817:Rabbits; D011826:Radial Nerve / Q000293:injuries / Q000503:physiopathology / Q000601:surgery; D011984:Sensory Receptor Cells / Q000503:physiopathology; D013536:Suture Techniques