Isolation of vasodilatory active flavonoids from the traditional remedy Satureja obovata.
In the course of screening natural products for smooth muscle relaxant properties, the effects of constituents of Satureja obovata var. obovata (Lamiaceae) on vascular reactivity experiments were studied. Bioassay-guided fractionation led to pure vasodilator compounds, identified by spectroscopic methods as the flavonoids: naringenin, eriodictyol, and luteolin. The relaxant effects induced by different extracts and fractions obtained on KCl (80 mM) and noradrenaline (NA) (10(-6) M) induced contractions on isolated rat aorta were selected as bioassay procedures. The sustained contraction induced by NA (10(-6) M) and K+ (80 mM) were relaxed by luteoline (98.7 +/- 0.7%; 40.30 +/- 2.4%), naringenin (12.41 +/- 1.8%; 3.05 +/- 0.3%), and eriodictyol (67.48 +/- 3.3%; 17.93 +/- 2.1%) each at the dose of 5 x 10(-5)M, respectively.
Sánchez de Rojas
V R
VR
Departamento de Farmacología, Facultad de Farmacia, Universidad Complutense, Madrid, Spain.
Somoza
B
B
Ortega
T
T
Villar
A M
AM
D000818:Animals; D001011:Aorta / Q000187:drug effects / Q000502:physiology; D044950:Flavanones; D005419:Flavonoids / Q000302:isolation & purification* / Q000494:pharmacology; D066298:In Vitro Techniques; D047311:Luteolin; D009119:Muscle Contraction / Q000187:drug effects*; D009126:Muscle Relaxation / Q000187:drug effects; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000502:physiology*; D009638:Norepinephrine / Q000494:pharmacology; D008517:Phytotherapy; D010946:Plants, Medicinal; D051381:Rats; D013030:Spain; D014665:Vasodilator Agents / Q000302:isolation & purification* / Q000494:pharmacology
D044950:Flavanones; D005419:Flavonoids; D014665:Vasodilator Agents; C005273:naringenin; D047311:Luteolin; C007619:eriodictyol; D009638:Norepinephrine