Reduction of Saccharomyces cell adhesion by liquid mechanical vibration.
The effect of liquid mechanical vibration on the adhesion of Saccharomyces cerevisiae cells to the internal glass surface of a pipette was studied using a 25 Hz vibration source. The maximum vibration amplitude was 1.06 mm (peak to peak) along the pipette direction. Relative movements between the pipette and yeast suspension in it were produced by vibration and reduced the cell adhesion. The reduction in adhesion was affected by both vibration amplitude and suspension pH. Analysis showed that in routine cell counts, cell adhesion to the pipette wall was a significant error source. The construction of a vibration device for routine cell count work appears feasible.
Jiang
C
C
Department of Biological Science and Biotechnology, Hangzhou University, People's Republic of China.
Yu
H
H
Ding
Z
Z
Ma
Y
Y
D002448:Cell Adhesion; D015169:Colony Count, Microbial; D005898:Glass; D006863:Hydrogen-Ion Concentration; D012440:Saccharomyces / Q000502:physiology*; D014732:Vibration