[Endothelin-1 levels in sputum and plasma of asthmatic patient after allergen provocation].
Recently, endothelin-1 (ET-1) has a potent of contractive effect to bronchial smooth muscle, and it suggests that ET-1 contributes to pathophysiology of bronchial asthma. To study the role of endothelin-1 (ET-1) in allergic asthma, we measured ET concentration in plasma and in sputum of nine allergic subjects following allergen provocation by using sandwich-enzyme immunoassay (EIA). ET-1 concentration in plasma were higher during IAR (2.31 +/- 0.24 pg/ml) (0.05 < p < 0.1) and LAR (2.55 +/- 0.27 pg/ml) (0.05 < p < 0.1) than prechallenge value (1.99 +/- 0.23 pg/ml) but there was no significant difference. On the other hand ET-1 concentration in sputum were significantly higher during IAR (14.75 +/- 2.77 pg/ml) (p < 0.05) and LAR (18.51 +/- 4.57 pg/ml) (p < 0.05) than prechallenge values (4.29 +/- 2.55 pg/ml). Thus these results suggest that ET-1 play a role of allergic bronchoconstraction (IAR and LAR) after allergen challenge.
Kurokawa
M
M
First Department of Internal Medicine, School of Medicine, Showa University, Japan.
Konno
S
S
Gonokami
Y
Y
Kouno
Y
Y
Kawazu
K
K
Okamoto
M
M
Adachi
M
M
D000293:Adolescent; D000328:Adult; D000485:Allergens / Q000276:immunology*; D001249:Asthma / Q000276:immunology* / Q000503:physiopathology; D015415:Biomarkers / Q000032:analysis; D001985:Bronchial Provocation Tests; D016084:Bronchoconstriction; D016232:Endothelins / Q000032:analysis*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D013183:Sputum / Q000737:chemistry
D000485:Allergens; D015415:Biomarkers; D016232:Endothelins