Insulin-like growth factor-I (IGF-I) mRNA in the small intestine of suckling and adult rats.
The presence and cellular localization of insulin-like growth factor-I (IGF-I) mRNA in the small intestine of suckling and adult rats was studied. A sensitive reverse transcription (RT) competitive-polymerase chain reaction (PCR) revealed IGF-I gene expression in both age groups. Adult tissue contained 3-fold higher levels of IGF-I mRNA in comparison with sucklings. Using an in situ hybridization technique, IGF-I transcripts were localized mainly in enterocytes and goblet cells in the intestinal crypts of adult rats. By using this technique, IGF-I mRNA was not detected in jejunum of 12-day-old rats.
Dvorák
B
B
Department of Pediatrics and Steele Memorial Children's Research Center, University of Arizona, Tucson, AZ 85724, USA. dvorakb@ccit.arizona.edu
Stephana
A L
AL
Holubec
H
H
Williams
C S
CS
Philipps
A F
AF
Koldovskoý
O
O
D000818:Animals; D000831:Animals, Newborn; D001483:Base Sequence; D017931:DNA Primers; D007334:Insulin-Like Growth Factor I / Q000235:genetics / Q000378:metabolism*; D007421:Intestine, Small / Q000378:metabolism* / Q000648:ultrastructure; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis*; D051381:Rats; D017207:Rats, Sprague-Dawley
D017931:DNA Primers; D012333:RNA, Messenger; D007334:Insulin-Like Growth Factor I