Fluorometric riboflavin titration in plasma by riboflavin-binding apoprotein as a method for vitamin B2 status assessment.
A method for plasma riboflavin determination by riboflavin-binding apoprotein titration has been proposed for vitamin B2 status evaluation. The method is based on the formation of riboflavin-apoprotein complex accompanied by full loss of fluorescence peculiar to free riboflavin. The data obtained have demonstrated a correlation with indicators of the vitamin B2 status such as urinary excretion, erythrocyte content, and stimulation of the glutathione reductase activity by flavin adenine dinucleotide. Levels > 6 ng/ml blood plasma may be considered to be a criterion for normal vitamin B2 supply.
Kodentsova
V M
VM
Laboratory of Vitamin and Mineral Metabolism, Institute of Nutrition, Russian Academy of Medical Sciences, Moscow, Russia.
Vrzhesinskaya
O A
OA
Spirichev
V B
VB
D000293:Adolescent; D000328:Adult; D001059:Apoproteins / Q000097:blood*; D002648:Child; D002675:Child, Preschool; D005260:Female; D005182:Flavin-Adenine Dinucleotide / Q000494:pharmacology; D006801:Humans; D008297:Male; D008875:Middle Aged; D009752:Nutritional Status; D012256:Riboflavin / Q000097:blood* / Q000652:urine; D013050:Spectrometry, Fluorescence
D001059:Apoproteins; D005182:Flavin-Adenine Dinucleotide; D012256:Riboflavin