Detection and differentiation of epizootic haemorrhagic disease of deer and bluetongue viruses by serogroup-specific sandwich ELISA.
A serogroup specific sandwich ELISA was developed for the detection of epizootic haemorrhagic disease of deer viruses (EHDV) in infected insects and tissue culture preparations. Polyclonal rabbit antiserum against purified EHDV core particles was used to capture viral antigen and specific binding detected using guinea pig antisera against EHDV core particles followed by anti-guinea pig immunoglobulin enzyme-labelled conjugate. The assay is EHDV specific and detects all 8 serotypes. No cross-reactions were found with related viruses such as bluetongue (BTV), Palyam, Tilligery or African horse sickness virus (AHSV). A similar serogroup specific sandwich ELISA was also developed for BTV. The assays showed a similar sensitivity in detecting the respective EHDV or BTV antigens in a pool of 500 midges where only 2 were infected. These assays allow a simple and rapid means of detecting and differentiating members of these closely related serogroups. The sensitivity of the tests will allow more extensive studies on vector competence and virus/vector distribution.
Thevasagayam
J A
JA
Institute for Animal Health, Pirbright, Woking, Surrey, UK.
Wellby
M P
MP
Mertens
P P
PP
Burroughs
J N
JN
Anderson
J
J
D000818:Animals; D001820:Bluetongue virus / Q000302:isolation & purification*; D002460:Cell Line; D002519:Ceratopogonidae / Q000821:virology*; D002642:Chick Embryo; D006224:Cricetinae; D003670:Deer; D004797:Enzyme-Linked Immunosorbent Assay; D006168:Guinea Pigs; D018164:Hemorrhagic Disease Virus, Epizootic / Q000302:isolation & purification*; D011817:Rabbits; D012680:Sensitivity and Specificity