[A method of detecting soluble immune complexes in tissue biopsies].
Pretreatment of cryostat slices of tissue biopsy specimens with 30-50% water ethanol mixture increases the rate of detection of immune complexes in the intercellular substance of the epidermis to 100% in patients with acantholytic pemphigus. This method permitted the detection of immune complexes in all the examined cases with benign familial Hailey-Hailey pemphigus, which has never been reported heretofore. The authors believe that 30-50% water-ethanol mixture is capable of soft denaturation of proteins, stabilizes the slightly affine antibody-antigen relations, and aggregates soluble immune complexes into larger protein formations, this preventing their washing out from tissues.
Beletskaia
L V
LV
Makhneva
N V
NV
D000936:Antigen-Antibody Complex / Q000032:analysis*; D001706:Biopsy; D019085:Fluorescent Antibody Technique, Direct; D006801:Humans; D010392:Pemphigus / Q000276:immunology* / Q000473:pathology; D016506:Pemphigus, Benign Familial / Q000276:immunology / Q000473:pathology; D012867:Skin / Q000276:immunology / Q000473:pathology; D012995:Solubility
D000936:Antigen-Antibody Complex
Sposob vyiavleniia rastvorimykh immunnykh kompleksov v bioptatakh tkaneĭ.