Determination of heterocyclic amines by pneumatically assisted electrospray liquid chromatography-mass spectrometry.
Electrospray ionization mass spectrometry was applied to the study of the amines IQ, Trp-P-1, Trp-P-2, PhIP and A alpha C and the co-mutagens harman and norharman. The results obtained on a triple quadrupole mass spectrometer equipped with a pneumatically assisted electrospray source are reported. The chromatographic conditions were optimized with a reversed-phase column (1 mm I.D.) using acetonitrile-5 mM ammonium acetate (pH 6.7) (50:50) as the mobile phase at a flow-rate of 50 microliters min-1. Different parameters influencing the mass spectra were investigated. For these compounds [M + H]+ in the positive-ion mode and also some fragments produced through collisionally activated decomposition in the interface were observed. Detection limits of 5.4-44 pg were obtained for standard solutions of these amines. Analysis of a meat extract was performed by HPLC-MS using single-ion monitoring after a solid-phase extraction clean-up.
Galceran
M T
MT
Department de Química Analítica, Universitat de Barcelona, Spain.
Moyano
E
E
Puignou
L
L
Pais
P
P
D000588:Amines / Q000032:analysis*; D000818:Animals; D002138:Calibration; D002243:Carbolines / Q000032:analysis; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000706:statistics & numerical data; D006247:Harmine / Q000031:analogs & derivatives / Q000032:analysis; D006571:Heterocyclic Compounds / Q000032:analysis*; D013058:Mass Spectrometry / Q000379:methods* / Q000706:statistics & numerical data; D008460:Meat / Q000032:analysis*; D009153:Mutagens / Q000032:analysis
D000588:Amines; D002243:Carbolines; D006571:Heterocyclic Compounds; D009153:Mutagens; D006247:Harmine; C005010:harman; C010262:norharman; C019132:3-amino-1,4-dimethyl-5H-pyrido(4,3-b)indole