Meal-feeding specifically induces obese mRNA expression.
Meal-feeding caused a rapid and specific induction of ob mRNA levels in rat adipose tissue. A high-carbohydrate meal caused a mean 5-fold increase in ob mRNA within 3 h after feeding and almost reached the levels of expression seen in rats fed ad lib. After the meal ob mRNA declined with a half-life of less than 2 h. The increase in ob mRNA in response to the meal was prevented by prior injection of actinomycin-D, an inhibitor of transcription. None of the mRNAs for other genes involved in lipid metabolism (lipoprotein lipase, fatty acid synthase, malic enzyme, hormone-sensitive lipase, S14 or adipsin) showed any significant difference between pre-meal and post-meal levels.
Thompson
M P
MP
Department of Biochemistry, University of Otago, Dunedin, New Zealand. maryt@sanger.otago.ac.nz
D000199:Actins / Q000096:biosynthesis; D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D015342:DNA Probes; D003609:Dactinomycin / Q000494:pharmacology; D004435:Eating / Q000502:physiology*; D004822:Epididymis; D005786:Gene Expression Regulation / Q000187:drug effects; D020738:Leptin; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D009765:Obesity / Q000235:genetics*; D016133:Polymerase Chain Reaction; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar; D014158:Transcription, Genetic / Q000187:drug effects
D000199:Actins; D017931:DNA Primers; D015342:DNA Probes; D020738:Leptin; D012333:RNA, Messenger; D003609:Dactinomycin