CTL lysis: there is a hyperbolic relation of killing rate to exocytosable granzyme A for highly cytotoxic murine cytotoxic T lymphocytes.
The lysis of susceptible targets by efficient cytotoxic T lymphocytes (CTL) increases both with time and with the ratio of CTL to target. Simple methods for calculating a killing rate constant from the time dependence of killing and for calculating the relation of the killing rate constant to the concentration of exocytosable granzyme A are given. Application of these methods to the killing of target cells by the highly efficient mouse CTL AR1 is presented. AR1 needed granzyme A for efficient killing. AR1 contained sufficient exocytosable granzyme A to kill at about 80% of the rate possible at infinite exocytosable granzyme A.
Poe
M
M
Department of Immunology Research, Merck Research Laboratories, Rahway, NJ 07065-0900, USA.
Wu
J K
JK
Talento
A
A
Koo
G C
GC
D000818:Animals; D002460:Cell Line; D003601:Cytotoxicity Tests, Immunologic; D003602:Cytotoxicity, Immunologic; D005089:Exocytosis / Q000276:immunology*; D053804:Granzymes; D007700:Kinetics; D051379:Mice; D012697:Serine Endopeptidases / Q000276:immunology / Q000378:metabolism*; D013602:T-Lymphocytes, Cytotoxic / Q000201:enzymology* / Q000276:immunology*
D053804:Granzymes; D012697:Serine Endopeptidases