title: Accelerated evolution of Trimeresurus okinavensis venom gland phospholipase A2 isozyme-encoding genes.
abstract: Three Trimeresurus okinavensis (To; himehabu snake, Crotalinae) venom gland phospholipase A2 (PLA2) isozymeencoding genes, gPLA2-o1, gPLA2-o2 and gPLA2-o3, were isolated from its genomic DNA library. The nucleotide (nt) sequence analysis revealed that two of the three genes (gPLA2-o2 and gPLA2-o3) occasionally have been converted to inactivated genes by introduction of one base insertion or substitution. It was confirmed from Southern blot analysis that the To haploid genome contains only three venom gland PLA2 isozyme genes herein isolated. Comparison of these genes showed that nonsynonymous nt substitutions have occurred more frequently than synonymous nt substitutions in the protein-coding regions, except for the signal-peptide coding domain, implying that To venom gland PLA2 isozyme genes have evolved via accelerated evolution. Such an evolutionary feature of To venom gland PLA2 isozyme genes proves the general universality of accelerated evolution previously drawn for venom gland PLA2 isozyme genes of other crotalinae snakes. The variability in the mature protein-coding regions of three To venom gland PLA2 isozyme genes appears to have been brought about by natural selection for point mutations.
authors.affiliation: Department of Chemistry, Faculty of Science, Kyushu University, Fukuoka, Japan.
authors.forename: I
authors.initials: I
authors.lastname: Nobuhisa
authors.forename: K
authors.initials: K
authors.lastname: Nakashima
authors.forename: M
authors.initials: M
authors.lastname: Deshimaru
authors.forename: T
authors.initials: T
authors.lastname: Ogawa
authors.forename: Y
authors.initials: Y
authors.lastname: Shimohigashi
authors.forename: Y
authors.initials: Y
authors.lastname: Fukumaki
authors.forename: Y
authors.initials: Y
authors.lastname: Sakaki
authors.forename: S
authors.initials: S
authors.lastname: Hattori
authors.forename: H
authors.initials: H
authors.lastname: Kihara
authors.forename: M
authors.initials: M
authors.lastname: Ohno
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003435:Crotalid Venoms / Q000201:enzymology* / Q000235:genetics; D018076:DNA, Complementary / Q000302:isolation & purification; D019143:Evolution, Molecular; D006238:Haploidy; D007527:Isoenzymes / Q000235:genetics*; D008969:Molecular Sequence Data; D010741:Phospholipases A / Q000235:genetics*; D054467:Phospholipases A2; D010802:Phylogeny; D017841:Trimeresurus / Q000235:genetics*
chemical_list: D003435:Crotalid Venoms; D018076:DNA, Complementary; D007527:Isoenzymes; C029748:Trimeresurus venoms; D010741:Phospholipases A; D054467:Phospholipases A2