title: Possible involvement of calpain in the growth of estrogen receptor positive breast cancer cells.
abstract: Calpain (Ca2(+)-activated neutral protease, EC 3.4.22.17) has been reported to hydrolyze the estrogen receptor (ER). However, there has been no report available regarding the role of calpain in the growth of breast cancer cells. To investigate the role of calpain in the growth of various breast cancer cell lines, we employed a synthetic peptide, calpeptin, which is a cell permeable specific inhibitor of calpain. Calpeptin inhibited the cell growth of ER positive breast cancer cells, such as MCF-7, T-47D, and ZR-75-1 in a dose dependent manner in the presence of E2. However, the growth of ER negative breast cancer cells, MDA-MB-231, was not inhibited by calpeptin. It is suggested that calpain plays an important role in the growth of ER positive breast cancer cells.
authors.affiliation: Department of Surgical Oncology, Osaka University Medical School, Japan.
authors.forename: E
authors.initials: E
authors.lastname: Shiba
authors.forename: S
authors.initials: S
authors.lastname: Kim
authors.forename: M
authors.initials: M
authors.lastname: Fujitani
authors.forename: J I
authors.initials: JI
authors.lastname: Kambayashi
authors.forename: I
authors.initials: I
authors.lastname: Kawamura
authors.forename: S
authors.initials: S
authors.lastname: Tsujimoto
authors.forename: K
authors.initials: K
authors.lastname: Shimomura
authors.forename: Y
authors.initials: Y
authors.lastname: Tanji
authors.forename: T
authors.initials: T
authors.lastname: Taguchi
authors.forename: Y
authors.initials: Y
authors.lastname: Kimoto
authors.forename: M
authors.initials: M
authors.lastname: Izukura
authors.forename: S I
authors.initials: SI
authors.lastname: Takai
mesh_terms: D000818:Animals; D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology*; D002154:Calpain / Q000037:antagonists & inhibitors / Q000502:physiology*; D002455:Cell Division; D004151:Dipeptides / Q000378:metabolism / Q000494:pharmacology*; D005260:Female; D006801:Humans; D051381:Rats; D011960:Receptors, Estrogen / Q000378:metabolism; D014407:Tumor Cells, Cultured
chemical_list: D004151:Dipeptides; D011960:Receptors, Estrogen; C071482:calpeptin; D002154:Calpain