Electrotransformation of highly DNA-restrictive corynebacteria with synthetic DNA.
Highly DNA-restrictive Corynebacteria can be transformed with DNA made in vitro by PCR amplification of a sequence that contains the replication origin of pBL1, a plasmid common to many Corynebacteria. In all strains examined, the transformation efficiencies of PCR-synthetized DNA equal or improve the performances of heterologous DNA extracted from wild-type and dam(-)-dcm-strains of Escherichia coli. The transformation efficiencies obtained with PCR-made DNA may be high enough to permit its general application to experiments of gene integration.
Ankri
S
S
Institut de Génétique et Microbiologie, Université de Paris-Sud, Orsay, France.
Reyes
O
O
Leblon
G
G
D003352:Corynebacterium / Q000201:enzymology / Q000235:genetics*; D015254:DNA Modification Methylases / Q000235:genetics / Q000378:metabolism; D004262:DNA Restriction Enzymes / Q000378:metabolism*; D004269:DNA, Bacterial / Q000235:genetics*; D004274:DNA, Recombinant / Q000235:genetics*; D018274:Electroporation; D004926:Escherichia coli / Q000235:genetics; D029968:Escherichia coli Proteins; D008745:Methylation; D010957:Plasmids / Q000235:genetics*; D016133:Polymerase Chain Reaction; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific) / Q000235:genetics / Q000378:metabolism; D014169:Transformation, Bacterial
D004269:DNA, Bacterial; D004274:DNA, Recombinant; D029968:Escherichia coli Proteins; D015254:DNA Modification Methylases; C058693:Dam methyltransferase; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific); C499294:dam protein, E coli; D004262:DNA Restriction Enzymes