title: [Spermatozoa antigen SAA-1 and co-precipitating molecules: regulation of human acrosome reaction].
abstract: OBJECTIVE
  Examination of the regulation of the human sperm acrosome reaction as an example of ligand-induced exocytosis.
  
  
  METHODS
  Development of monoclonal antibodies (mab) against immunaffinity-purified sperm antigen SAA-1. Examination of cross-reactivity of the mabs to human tissues using immunohistochemistry. Examination of reactivity to endometrial carcinoma cell lines and to PC-12 cells by immunohistochemistry and by radioimmunoassay.
  
  
  RESULTS
  Mabs to immunoaffinity-purified SAA-1 recognize three coprecipitating molecules. Cross-reactivity was demonstrated to glandular epithelia exhibiting ligand-induced exocytosis, and to endometrial carcinoma and PC-12 cells.
  
  
  CONCLUSIONS
  The proteins described could be components of the exocytosis-regulating machinery of human spermatozoa.
authors.affiliation: I. Frauenklinik, Ludwig-Maximilians-Universität, München.
authors.forename: C
authors.initials: C
authors.lastname: Brucker
authors.forename: G v
authors.initials: Gv
authors.lastname: Herder
authors.forename: A
authors.initials: A
authors.lastname: Keberle
authors.forename: G
authors.initials: G
authors.lastname: Kuhn
authors.forename: V
authors.initials: V
authors.lastname: Schlamp
authors.forename: G B
authors.initials: GB
authors.lastname: Lipford
mesh_terms: D000177:Acrosome / Q000502:physiology*; D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D002868:Chromosomal Proteins, Non-Histone / Q000302:isolation & purification / Q000502:physiology*; D005089:Exocytosis / Q000502:physiology*; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011247:Pregnancy; D013084:Sperm-Ovum Interactions / Q000502:physiology*; D013094:Spermatozoa / Q000502:physiology*
chemical_list: D000911:Antibodies, Monoclonal; D002868:Chromosomal Proteins, Non-Histone; C053886:spermatid transition proteins
vernacular_title: Spermatozoen-Antigen SAA-1 und kopräzipitierende Moleküle: Regulation der menschlichen Akrosomenreaktion.