The inhibitor protein of phosphorylated nitrate reductase from spinach (Spinacia oleracea) leaves is a 14-3-3 protein.
The inhibitor protein (IP) that inactivates spinach leaf NADH:nitrate reductase (NR) has been identified for the first time as a member of the eukaryotic 14-3-3 protein family based on three lines of evidence. First, the sequence of an eight amino acid tryptic peptide, obtained from immunopurified IP, matched that of a highly conserved region of the 14-3-3 proteins. Second, an authentic member of the 14-3-3 family, recombinant Arabidopsis GF14omega, caused inactivation of phospho-NR in a magnesium-dependent manner identical to IP. Third, an anti-GF14 monoclonal antibody cross-reacted with IP and anti-IP monoclonal antibodies cross-reacted with GF14omega.
Bachmann
M
M
U.S. Department of Agriculture, Agriculture Research Service, Raleigh, NC 27695-7631, USA.
Huber
J L
JL
Liao
P C
PC
Gage
D A
DA
Huber
S C
SC
D048948:14-3-3 Proteins; D000595:Amino Acid Sequence; D000818:Animals; D029681:Arabidopsis Proteins; D002135:Calcium-Binding Proteins / Q000494:pharmacology; D005260:Female; D015151:Immunoblotting; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D050895:Nitrate Reductase (NADH); D009565:Nitrate Reductases / Q000037:antagonists & inhibitors*; D010766:Phosphorylation; D018515:Plant Leaves / Q000201:enzymology; D010940:Plant Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D011233:Precipitin Tests; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000494:pharmacology; D018724:Spinacia oleracea / Q000201:enzymology*; D014446:Tyrosine 3-Monooxygenase
D048948:14-3-3 Proteins; D029681:Arabidopsis Proteins; D002135:Calcium-Binding Proteins; C087620:GF14 protein, Arabidopsis; D010940:Plant Proteins; D011506:Proteins; D011994:Recombinant Proteins; D014446:Tyrosine 3-Monooxygenase; D009565:Nitrate Reductases; D050895:Nitrate Reductase (NADH)