Observation of baker's yeast strains used in biotransformation by atomic force microscopy.
Different strains of baker's yeast (Saccharomyces cerevisiae) were imaged with an atomic force microscope (AFM). The images of uncoated and nonfixed samples were reproducible with high-constrast and nanometer-resolution. Molecules from the polysaccharide surface of the cell wall were pictured and the distance of atoms was measured. The preparation of samples was easy, suggesting that AFM is a useful tool in this type of analyses.
Pereira
R de S
Rde S
Instituto de Quimica, Biological Chemistry Laboratory, Universidade Estadual de Campinas, SP, Brazil.
Parizotto
N A
NA
Baranauskas
V
V
D001711:Biotransformation; D002473:Cell Wall / Q000378:metabolism / Q000648:ultrastructure; D018625:Microscopy, Atomic Force; D011134:Polysaccharides / Q000378:metabolism / Q000648:ultrastructure; D015203:Reproducibility of Results; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure
D011134:Polysaccharides