Elimination of the effects of stray light in measurements by total internal reflection aqueous fluorescence (TIRAF).
Total internal reflection aqueous fluorescence has been shown to be capable of achieving spatial resolution in surface contours of about 1 nm. When used with highly structured objects, errors in measurements can arise from light scattered either by the object or within the body of the microscope. We describe how these errors can be eliminated when studying surface contours of human platelets.
Gingell
D
D
Department of Anatomy & Developmental Biology, University College of London, U.K.
Heavens
O
O
D001792:Blood Platelets / Q000166:cytology*; D006801:Humans; D007834:Lasers; D008027:Light; D008433:Mathematics; D008856:Microscopy, Fluorescence / Q000379:methods; D017785:Photons; D012542:Scattering, Radiation