title: Factors conditioning detection of Taenia saginata antigens in faeces.
abstract: The conditions which optimize detection of T. saginata antigens in faeces were subject of examination. The most appropriate appeared diluent of PBS + 0.3% Tween 20. The highest effectiveness was achieved using capturing and detecting antibodies to surface antigens in comparison with somatic and metabolic ones. Two step centrifugation and final dilution of samples 1:10 (w:v) were recommended. The stability of freezed faecal supernatants after the first centrifugation was limited to 2 months. The sensitivity of capture ELISA established in our examinations allow to signalize 1 ng/ml antigen in high absorbance values. The antigens recognized by the test had MW bigger than 100,000.
authors.affiliation: Institute of Parasitology of the Polish Academy of Sciences, Warsaw, Poland.
authors.forename: B
authors.initials: B
authors.lastname: Machnicka
authors.forename: E
authors.initials: E
authors.lastname: Dziemian
authors.forename: C
authors.initials: C
authors.lastname: Zwierz
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000032:analysis*; D003551:Cysticercosis / Q000276:immunology / Q000469:parasitology*; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000469:parasitology*; D005615:Freezing; D006801:Humans; D011817:Rabbits; D012680:Sensitivity and Specificity; D013621:Taenia / Q000276:immunology / Q000302:isolation & purification*
chemical_list: D000947:Antigens, Helminth