Improving the engineered activity of mutants of clostridial glutamate dehydrogenase towards monocarboxylic substrates: substitution of Ala163 with glycine.
Wang
X G
XG
Department of Biochemistry, UCD. Belfield, Dublin 4; Eire UK.
Britton
L K
LK
Baker
P J
PJ
Rice
D W
DW
Engel
P C
PC
D000409:Alanine; D002264:Carboxylic Acids / Q000378:metabolism*; D003013:Clostridium / Q000201:enzymology*; D005969:Glutamate Dehydrogenase / Q000378:metabolism*; D005998:Glycine; D007700:Kinetics; D016297:Mutagenesis, Site-Directed; D009243:NAD / Q000378:metabolism; D017354:Point Mutation; D011994:Recombinant Proteins / Q000378:metabolism*; D013379:Substrate Specificity
D002264:Carboxylic Acids; D011994:Recombinant Proteins; D009243:NAD; D005969:Glutamate Dehydrogenase; D000409:Alanine; D005998:Glycine