title: Immunohistochemical detection of Bax and Bcl-2 proteins in gerbil hippocampus following transient forebrain ischemia.
abstract: Time-course expression of Bax and Bcl-2 proteins, identified as apoptosis-regulating molecules, was assessed in gerbil hippocampus following transient forebrain ischemia. Brain sections from animals sacrificed at 48, 72, 96 h and 7 days following 5 min ischemia were immunohistochemically evaluated using polyclonal antibodies specific for Bax and Bcl-2 proteins, respectively. The intensity of Bax expression in CA1 neurons increased with time and peaked at 72 h, and immediately disappeared at 96 h following 5 min ischemia. No expression of Bcl-2 in the CA1 neurons was recognized in all the time evaluated.
authors.affiliation: Department of Pathology, Gifu University School of Medicine, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Hara
authors.forename: T
authors.initials: T
authors.lastname: Iwai
authors.forename: M
authors.initials: M
authors.lastname: Niwa
authors.forename: T
authors.initials: T
authors.lastname: Uematsu
authors.forename: N
authors.initials: N
authors.lastname: Yoshimi
authors.forename: T
authors.initials: T
authors.lastname: Tanaka
authors.forename: H
authors.initials: H
authors.lastname: Mori
mesh_terms: D000818:Animals; D002545:Brain Ischemia / Q000276:immunology*; D016923:Cell Death / Q000276:immunology; D004247:DNA / Q000276:immunology; D005849:Gerbillinae; D006624:Hippocampus / Q000378:metabolism*; D007150:Immunohistochemistry; D011518:Proto-Oncogene Proteins / Q000276:immunology*; D019253:Proto-Oncogene Proteins c-bcl-2; D013997:Time Factors; D051028:bcl-2-Associated X Protein
chemical_list: D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; D004247:DNA