Identification and biosynthesis of fibrinogen in human uterine cervix carcinoma cells.
Fibrinogen has been detected in ME-180 human uterine cervix carcinoma cells, and synthesis of fibrinogen by ME-180 cells has been measured using [35S] L-methionine incorporation. Expression of mRNA for the B beta-chain of the fibrinogen in ME-180 cells has been identified by PCR-assisted mRNA amplification. The A alpha-, B beta-, and gamma-chains of fibrinogen synthesized by ME-180 cells were chemically and immunologically identical to those of plasma fibrinogen. These findings suggest that fibrinogen is present and synthesized in ME-180 human uterine cervix carcinoma cells, and that fibrinogen plays a role in these malignant tumor cells.
Lee
S Y
SY
Department of Natural Sciences, Catholic University Medical College, Seoul, Korea.
Lee
K P
KP
Lim
J W
JW
D001483:Base Sequence; D005260:Female; D005340:Fibrinogen / Q000032:analysis* / Q000096:biosynthesis; D006801:Humans; D008969:Molecular Sequence Data; D009361:Neoplasm Invasiveness; D014407:Tumor Cells, Cultured; D002583:Uterine Cervical Neoplasms / Q000378:metabolism* / Q000473:pathology
D005340:Fibrinogen