Rederivation of mice by means of in vitro fertilization and embryo transfer.
In vitro fertilization and embryo transfer were performed for rederivation of four strains of mice harbouring mouse hepatitis virus (MHV) and/or Pasteurella pneumotropica (P. pneumotropica). Superovulated oocytes were fertilized by preincubated cauda epididymis sperm in vitro. Fertilized eggs at 2-cell stage were transferred into the oviducts of specific pathogen free (SPF) recipients. Microbial examination of sperm and/or oocyte donors verified the presence of P. pneumotropica and/or of antibodies to MHV in all strains, but neither in the recipients nor in the offspring antibodies to MHV could they be detected. The results indicate that an in vitro fertilization-embryo transfer (IVF-ET) system is an effective and simple alternative to cesarean operation in infected mice.
Suzuki
H
H
Chugai Pharmaceutical Co., Ltd, Japan.
Yorozu
K
K
Watanabe
T
T
Nakura
M
M
Adachi
J
J
D000818:Animals; D000831:Animals, Newborn / Q000382:microbiology; D001947:Breeding / Q000379:methods*; D004624:Embryo Transfer / Q000379:methods*; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008822:Mice, Transgenic; D006517:Murine hepatitis virus / Q000302:isolation & purification*; D009865:Oocytes / Q000382:microbiology; D010325:Pasteurella / Q000302:isolation & purification*; D012107:Research Design; D013047:Specific Pathogen-Free Organisms; D013094:Spermatozoa / Q000382:microbiology