[Pathological studies on the anti-invasive character of IL-6 gene transfected leukemia cells].
FBL-3 Leukemia cells transfected with IL-6 gene were expanded in vitro and inoculated into C57BL/6 mice subcutaneously. Tumor growth was observed and histologic analyses of the tumors in situ and the liver, spleen and bone marrow were performed at 14, 21, 28 and 35 days after inoculation. The mice inoculated with wild-type FBL-3 leukemia cells were used as the control. We found that the tumor invasiveness in the mice inoculated with FBL-3-IL-6+ occurred later than in the control group. The survival time of experimental mice was longer than in the control mice. The results demonstrated that inoculation of IL-6 high-secreting FBL-3 inhibited invasiveness of the leukemia cells, suggesting that the IL-6 gene transfected FBL-3 cells can be used as a vaccine to treat leukemia. The mechanism of the anti-invasiveness of IL-6 gene transfected leukemia cells needs further study.
Ge
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L
Department of Immunology, Second Military Medical University, Shanghai.
Cao
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X
Ma
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D
D000818:Animals; D001853:Bone Marrow / Q000473:pathology; D015850:Interleukin-6 / Q000235:genetics*; D004915:Leukemia, Erythroblastic, Acute / Q000235:genetics / Q000473:pathology*; D017254:Leukemic Infiltration; D008099:Liver / Q000473:pathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009368:Neoplasm Transplantation; D013154:Spleen / Q000473:pathology; D014162:Transfection; D014407:Tumor Cells, Cultured
D015850:Interleukin-6