title: The activity of the high-affinity K+ uptake system Kdp sensitizes cells of Escherichia coli to methylglyoxal.
abstract: Expression of the Kdp system sensitizes cells to methylglyoxal (MG) whether this electrophile is added externally or is synthesized endogenously. The basis of this enhanced sensitivity is the maintenance of a higher cytoplasmic pH (pHi) in cells expressing Kdp. In such cells, MG elicits rapid cytoplasmic acidification via KefB and KefC, but the steady-state pHi attained is still too high to confer protection Lowering pHi further by incubation with acetate increases the sensitivity of cells to MG.
authors.affiliation: Department of Molecular & Cell Biology, University of Aberdeen, United Kingdom.
authors.forename: G P
authors.initials: GP
authors.lastname: Ferguson
authors.forename: A D
authors.initials: AD
authors.lastname: Chacko
authors.forename: C H
authors.initials: CH
authors.lastname: Lee
authors.forename: I R
authors.initials: IR
authors.lastname: Booth
authors.forename: C
authors.initials: C
authors.lastname: Lee
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism*; D017920:Antiporters / Q000378:metabolism; D001426:Bacterial Proteins / Q000378:metabolism; D002352:Carrier Proteins / Q000378:metabolism*; D027682:Cation Transport Proteins; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D029968:Escherichia coli Proteins; D006863:Hydrogen-Ion Concentration; D011188:Potassium / Q000378:metabolism*; D015221:Potassium Channels / Q000378:metabolism; D027961:Potassium-Hydrogen Antiporters; D011765:Pyruvaldehyde / Q000494:pharmacology*
chemical_list: D017920:Antiporters; D001426:Bacterial Proteins; D002352:Carrier Proteins; D027682:Cation Transport Proteins; D029968:Escherichia coli Proteins; D015221:Potassium Channels; D027961:Potassium-Hydrogen Antiporters; C069287:KefC protein, E coli; D011765:Pyruvaldehyde; D000251:Adenosine Triphosphatases; C058166:potassium translocating Kdp-ATPase, E coli; D011188:Potassium