title: Beta-1,2-linked oligomannosides inhibit Candida albicans binding to murine macrophage.
abstract: Interaction of Candida albicans with cells of the macrophage lineage was examined by using heat-killed (HK) and live yeast cells. Laminarin, an analogue of the cell wall beta-glucans, strongly inhibited HK yeasts adherence to J774 cell line but had no effect on live yeast binding. Phosphopeptidomannan (PPM) from Saccharomyces cerevisiae had a limited effect on the binding of both HK and live yeasts but significant inhibition was achieved by the use of C. albicans PPM. The role of beta-1,2-oligomannosides was examined with regard to their exclusive presence within C. albicans PPM. PPM acid labile beta-1,2-oligomannosides or a synthetic beta-1,2-mannotetraose, inhibited yeasts binding in a manner comparable to the original PPM. These latter results were confirmed by using mouse peritoneal macrophages, thus suggesting a general role for beta-1,2-oligomannosides in the adherence of the yeast to the macrophage membrane.
authors.affiliation: Unité INSERM 42, Domaine du CERTIA, Villeneuve d'Ascq, France.
authors.forename: C
authors.initials: C
authors.lastname: Fradin
authors.forename: T
authors.initials: T
authors.lastname: Jouault
authors.forename: A
authors.initials: A
authors.lastname: Mallet
authors.forename: J M
authors.initials: JM
authors.lastname: Mallet
authors.forename: D
authors.initials: D
authors.lastname: Camus
authors.forename: P
authors.initials: P
authors.lastname: Sinaÿ
authors.forename: D
authors.initials: D
authors.lastname: Poulain
mesh_terms: D000818:Animals; D002176:Candida albicans / Q000187:drug effects / Q000502:physiology*; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D006358:Hot Temperature; D008264:Macrophages / Q000187:drug effects / Q000502:physiology*; D008351:Mannans / Q000494:pharmacology; D051379:Mice; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000494:pharmacology*; D010587:Phagocytosis / Q000187:drug effects*; D010748:Phosphopeptides / Q000494:pharmacology; D012441:Saccharomyces cerevisiae
chemical_list: D008351:Mannans; D009844:Oligosaccharides; D010748:Phosphopeptides; C028569:oligomannoside; C072108:phosphopeptidomannan