Identification of cryptic peanut agglutinin-reactive sites in human lipoprotein(a).
After treatment of human lipoprotein(a) (Lp(a)) with neuraminidase, formerly cryptic sites became available for binding to peanut agglutinin (PNA) lectin and a Thomsen-Friedenreich antigen (T-antigen)-specific monoclonal antibody. The PNA-reactive sites were localized to the apo(a) moiety of Lp(a) and O-specific carbohydrate side chains. Lp(a) with larger isoforms of apo(a) contained more potential PNA reactivity per molecule of Lp(a) apoB than did smaller isoforms. Very low density lipoproteins (VLDL), low density lipoproteins (LDL), and high density lipoproteins (HDL) did not contain comparable amounts of the cryptic PNA-reactive sites.
Lee
L T
LT
Department of Physiology, Louisiana State University Medical Center, New Orleans 70112, USA.
Scheer
W D
WD
Thompson
J J
JJ
D015295:Antigens, Tumor-Associated, Carbohydrate / Q000737:chemistry / Q000378:metabolism; D001054:Apolipoproteins A / Q000737:chemistry / Q000378:metabolism; D001665:Binding Sites; D002236:Carbohydrate Conformation; D004587:Electrophoresis, Agar Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006026:Glycoside Hydrolases / Q000494:pharmacology; D006031:Glycosylation; D006801:Humans; D015151:Immunoblotting; D018082:Kringles; D037102:Lectins / Q000378:metabolism*; D017270:Lipoprotein(a) / Q000737:chemistry* / Q000378:metabolism*; D009439:Neuraminidase / Q000494:pharmacology; D019887:Peanut Agglutinin
D015295:Antigens, Tumor-Associated, Carbohydrate; D001054:Apolipoproteins A; D037102:Lectins; D017270:Lipoprotein(a); D019887:Peanut Agglutinin; C016467:Thomsen-Friedenreich antigen; D006026:Glycoside Hydrolases; C020668:glycanase; D009439:Neuraminidase