dup(10q) lacking alpha-satellite DNA in bone marrow cells of a patient with acute myeloid leukemia.
A marker chromosome was identified in leukemic cells on an AML patient. The G-banding pattern resembled on i(10q), but its centromeric position was not clear; in some cells it had a telocentric shape, in others a metacentric or acentric shape. The origin of the marker chromosome was confirmed by FISH, using chromosome-10-specific painting. To determine the centromeric position, C-banding and alpha-satellite probes were applied in FISH, and none of them gave a positive signal. Despite the absence of the centromeric alpha-satellite sequences and the constricted feature of the centromere, the essential centromeric activity was retained in this chromosome, namely, the separation of sister chromatids in anaphase.
Abeliovich
D
D
Department of Human Genetics, Hadassah University Hospital, Hebrew University, Hadassah Medical School, Jerusolem, Israel.
Yehuda
O
O
Ben-Neriah
S
S
Kapelushnik
Y
Y
Ben-Yehuda
D
D
D000293:Adolescent; D001853:Bone Marrow / Q000473:pathology; D002869:Chromosome Aberrations; D007446:Chromosome Inversion; D002879:Chromosomes, Human, Pair 10; D004276:DNA, Satellite / Q000032:analysis*; D005819:Genetic Markers; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D015470:Leukemia, Myeloid, Acute / Q000235:genetics*; D008297:Male
D004276:DNA, Satellite; D005819:Genetic Markers