Purification and N-terminal amino acid sequence of Enterocin CRL 35, a 'pediocin-like' bacteriocin produced by Enterococcus faecium CRL 35.
Enterocin CRL 35, a bacteriocin produced by Enterococcus faecium CRL 35 that inhibits food-borne pathogens, was purified by precipitation with (NH4)2SO4, gel filtration, ion exchange and reverse phase chromatography. The partial N-terminal amino acid sequence indicated a strong homology with other 'pediocin-like bacteriocins' previously described.
Farías
M E
ME
Departamento de Bioquímica de la Nutrición, Instituto Superior de Investigaciones Biologicas (INSIBIO), Tucumán, Argentina.
Farías
R N
RN
de Ruiz Holgado
A P
AP
Sesma
F
F
D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000302:isolation & purification*; D001430:Bacteriocins / Q000302:isolation & purification*; D001952:Bridged-Ring Compounds / Q000737:chemistry / Q000302:isolation & purification; D016984:Enterococcus faecium / Q000378:metabolism*; D008969:Molecular Sequence Data
D000900:Anti-Bacterial Agents; D001430:Bacteriocins; D001952:Bridged-Ring Compounds; C012306:enterocin