title: A novel member of the rho family of small GTP-binding proteins is specifically required for cytokinesis.
abstract: Several members of the rho/rac family of small GTP-binding proteins are known to regulate the distribution of the actin cytoskeleton in various subcellular processes. We describe here a novel rac protein, racE, which is specifically required for cytokinesis, an actomyosin-mediated process. The racE gene was isolated in a molecular genetic screen devised to isolate genes required for cytokinesis in Dictyostelium. Phenotypic characterization of racE mutants revealed that racE is not essential for any other cell motility event, including phagocytosis, chemotaxis, capping, or development. Our data provide the first genetic evidence for the essential requirement of a rho-like protein, specifically in cytokinesis, and suggest a role for these proteins in coordinating cytokinesis with the mitotic events of the cell cycle.
authors.affiliation: Department of Cell Biology, Duke University Medical Center, Durham, North Carolina 27710, USA.
authors.forename: D A
authors.initials: DA
authors.lastname: Larochelle
authors.forename: K K
authors.initials: KK
authors.lastname: Vithalani
authors.forename: A
authors.initials: A
authors.lastname: De Lozanne
mesh_terms: D000199:Actins / Q000032:analysis; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002455:Cell Division; D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000032:analysis; D004023:Dictyostelium / Q000166:cytology* / Q000235:genetics / Q000502:physiology; D019204:GTP-Binding Proteins / Q000235:genetics* / Q000502:physiology; D005800:Genes, Fungal / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D009218:Myosins / Q000032:analysis; D010641:Phenotype; D010802:Phylogeny; D012331:RNA, Fungal / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013172:Spores, Fungal; D020744:rac GTP-Binding Proteins
chemical_list: D000199:Actins; D004271:DNA, Fungal; D012331:RNA, Fungal; D012333:RNA, Messenger; C100414:RacE protein, Dictyostelium discoideum; D019204:GTP-Binding Proteins; D009218:Myosins; D020744:rac GTP-Binding Proteins