Interaction of RGD liposomes with platelets.
The interactions of platelets and liposomes with the tripeptide arginine-glycine-aspartic acid (RGD) as a surface ligand (RGD liposomes) were studied. The results suggest that the presence of the RGD ligand on the liposomes results in receptor-mediated mixing of lipid and aqueous contents of the liposomes and platelets. Mixing of the lipid and aqueous contents of RGD liposomes with platelets is approximately 4-9 times and 3-4 times greater than that for unlabeled liposomes, respectively. Measurements of the cytoplasmic free Ca2+ concentration in platelets, [Ca2+]i, show that the RGD liposomes have little effect on [Ca2+]i.
Nishiya
T
T
Artificial Cells and Organs Research Centre, McGill University, Montreal, Quebec, Canada.
Sloan
S
S
D000818:Animals; D001792:Blood Platelets / Q000187:drug effects / Q000502:physiology*; D002118:Calcium / Q000097:blood*; D002417:Cattle; D004134:Dimyristoylphosphatidylcholine; D004337:Drug Carriers; D005456:Fluorescent Dyes; D016257:Fura-2; D066298:In Vitro Techniques; D007700:Kinetics; D008081:Liposomes; D009842:Oligopeptides / Q000008:administration & dosage / Q000494:pharmacology*; D010715:Phosphatidylglycerols
D004337:Drug Carriers; D005456:Fluorescent Dyes; D008081:Liposomes; D009842:Oligopeptides; D010715:Phosphatidylglycerols; C047981:arginyl-glycyl-aspartic acid; C002773:dimyristoylphosphatidylglycerol; D002118:Calcium; D016257:Fura-2; D004134:Dimyristoylphosphatidylcholine