title: Detection of antibodies to Staphylococcus aureus in water buffalo milk by flow cytometry.
abstract: An assay has been developed to detect antibodies to Staphylococcus aureus in water buffalo milk by flow cytometry. The method was the protein A-deficient strain Wood 46 of S aureus incubated with milk samples and fluorescein-labelled rabbit anti-water buffalo antiserum. The assay can detect antibodies when the pathogen is not detectable by bacterial tests and can determine the antibody titre directly on undiluted samples.
authors.affiliation: University of Naples, School of Agriculture, Italy.
authors.forename: L
authors.initials: L
authors.lastname: D'Apice
authors.forename: D
authors.initials: D
authors.lastname: Fenizia
authors.forename: R
authors.initials: R
authors.lastname: Capparelli
authors.forename: F
authors.initials: F
authors.lastname: Scala
authors.forename: D
authors.initials: D
authors.lastname: Iannelli
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis*; D002020:Buffaloes; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D008892:Milk / Q000276:immunology* / Q000382:microbiology*; D011817:Rabbits; D012680:Sensitivity and Specificity; D013211:Staphylococcus aureus / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial