title: Expression and distribution of CD9 in myelin of the central and peripheral nervous systems.
abstract: CD9 is a member of the newly identified tetra-membrane-spanning protein family. We show here that CD9 is a constituent of myelin in the central and peripheral nervous systems. Expression of CD9 was detected in human cerebral white matter and sciatic nerve by Northern and Western blotting. Myelin in the central and peripheral nervous systems was strongly stained with a monoclonal antibody against human CD9 antigen in paraffin-embedded sections. CD9 was detected in adult nervous tissue but not in developing brain at less than 20 weeks of gestation. Immunohistochemical studies indicated that expression of CD9 is correlated with myelination and is somewhat delayed compared with expression of myelin basic protein, a major component protein of myelin. In the central nervous system, CD9 was detected along the outermost membrane of compact myelin but not inside compact myelin or the periaxonal region. Although the membrane-anchored form of heparin-binding epidermal-growth-factor-like growth factor (proHB-EGF), which is identical to the diphtheria toxin receptor, forms a complex with CD9 in some human and monkey cell lines, proHB-EGF was not detected in myelin immunocytochemically. The distribution of CD9 in the outer surface of myelin and its relatively late developmental appearance suggest that CD9 may interact with the extracellular matrix or cell adhesion molecules and participate in the maintenance of the entire myelin sheath.
authors.affiliation: Department of Pathology, St. Mary's Hospital, Kurume University, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Nakamura
authors.forename: R
authors.initials: R
authors.lastname: Iwamoto
authors.forename: E
authors.initials: E
authors.lastname: Mekada
mesh_terms: D000375:Aging / Q000378:metabolism; D015703:Antigens, CD / Q000096:biosynthesis* / Q000648:ultrastructure; D001923:Brain Chemistry; D002490:Central Nervous System / Q000196:embryology / Q000378:metabolism*; D004815:Epidermal Growth Factor / Q000378:metabolism; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008562:Membrane Glycoproteins / Q000096:biosynthesis* / Q000648:ultrastructure; D016253:Microscopy, Immunoelectron; D009186:Myelin Sheath / Q000378:metabolism* / Q000648:ultrastructure; D017933:Peripheral Nervous System / Q000196:embryology / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D060245:Tetraspanin 29
chemical_list: D015703:Antigens, CD; C554869:CD9 protein, human; D008562:Membrane Glycoproteins; D012333:RNA, Messenger; D060245:Tetraspanin 29; D004815:Epidermal Growth Factor