title: Early detection of metastasis by alterations in the cellular immune system in the murine liver and blood.
abstract: We investigated the reaction of the cellular immune system of liver and blood in the C57BL/6 mouse to a metastasizing Lewis lung carcinoma. The cellular immune system of the liver consists of mature and immature macrophages, B-cells, T-cells including their subpopulations, and natural killer cells, and their percentage frequencies differ significantly from those in the corresponding mononuclear blood cell (MBC) compartment. This suggests that the hepatic immune cells represent a system with autonomous function showing a typical homing of its members. Imminent metastasis to the liver is signalled by impressive alterations in the percentage frequencies of nonparenchymal liver cells (NPLC). There are a dramatic loss of mature macrophages, an increase in immature macrophages, a reduction of T-helper cells leading to a low CD4/CD8 ratio, and an increase in natural killer cells. In the blood, the corresponding precursor cells show comparable changes with a delay of at least 2 days. Early metastasis is accompanied by a significant increase in mononuclear NPLC producing tumour necrosis factor alpha. The alterations in percentage frequencies of the NPLC during tumour metastasis differ markedly from the changes in these cells in the liver during endotoxinaemia.
authors.affiliation: Pathologisches Institut, Universität, Freiburg, Germany.
authors.forename: N
authors.initials: N
authors.lastname: Freudenberg
authors.forename: P
authors.initials: P
authors.lastname: Rahner
authors.forename: C
authors.initials: C
authors.lastname: Darda
authors.forename: A
authors.initials: A
authors.lastname: Kiss
authors.forename: G
authors.initials: G
authors.lastname: Veres
authors.forename: T
authors.initials: T
authors.lastname: Nees
authors.forename: R
authors.initials: R
authors.lastname: Lamers
authors.forename: U N
authors.initials: UN
authors.lastname: Riede
authors.forename: C
authors.initials: C
authors.lastname: Kortsik
authors.forename: M
authors.initials: M
authors.lastname: Schubert
authors.forename: K
authors.initials: K
authors.lastname: Frenzer-Welle
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D016516:CD4-CD8 Ratio; D002452:Cell Count; D005260:Female; D007150:Immunohistochemistry; D008099:Liver / Q000276:immunology* / Q000648:ultrastructure; D008113:Liver Neoplasms / Q000276:immunology* / Q000556:secondary*; D008175:Lung Neoplasms / Q000276:immunology*; D018655:Lymphocyte Count; D008264:Macrophages / Q000276:immunology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008854:Microscopy, Electron; D013601:T-Lymphocytes / Q000276:immunology; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis
chemical_list: D014409:Tumor Necrosis Factor-alpha