High-concentration ethanol production from cooked corn starch by using medium-temperature cooking process.
An improved process for high-concentration ethanol production from cooked corn starch by Saccharomyces sp. W4 has been well developed. Simultaneous cooking and liquefaction of corn starch were performed by using thermophilic alpha-amylase at 95 degrees to 105 degrees C. The mash was then saccharified at 60 degrees C by using high-efficiency glucoamylase. Saccharomyces sp. W4 could produce 18.3% (v/v) ethanol at 30 degrees C within 60 hrs, with 1.2% reducing sugar and 4.1% total sugar remaining in the fermented mash. It was found that ammonium sulfate could accelerate the fermentation rate. When 0.9 g of ammonium sulfate was added to 280 mL of the fermentation media, 18.9% (v/v) ethanol could be reached in the mash after 50 hrs of fermentation, leaving 0.27% reducing sugar and 3.1% total sugar in the fermented media.
Chi
Z
Z
Department of Microbiology, Shandong University, Jinan, China.
Liu
J
J
Xu
P
P
D000645:Ammonium Sulfate / Q000494:pharmacology; D003296:Cooking; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation / Q000187:drug effects / Q000502:physiology; D005516:Food Microbiology; D005087:Glucan 1,4-alpha-Glucosidase / Q000378:metabolism / Q000494:pharmacology; D012440:Saccharomyces / Q000201:enzymology; D013213:Starch / Q000737:chemistry* / Q000378:metabolism; D013696:Temperature; D000516:alpha-Amylases / Q000378:metabolism / Q000494:pharmacology
D000431:Ethanol; D013213:Starch; D000516:alpha-Amylases; D005087:Glucan 1,4-alpha-Glucosidase; D000645:Ammonium Sulfate