A new approach for creating double-stranded DNA biosensors.
The principle of 'sandwich'-type biosensors based on liquid-crystalline dispersions formed from [DNA-polycation] complexes is outlined. These biosensors will find application in the determination of a range of compounds and physical factors that affect the ability of a given polycationic molecule to maintain intermolecular crosslinks between neighbouring DNA molecules. In the case of liquid-crystalline dispersions formed from [DNA-protamine] complexes, the lowest concentration of hydrolytic enzyme (trypsin) detectable was approximately 10-(14) M.
Skuridin
S G
SG
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia.
Yevdokimov
Y M
YM
Efimov
V S
VS
Hall
J M
JM
Turner
A P
AP
D000818:Animals; D015374:Biosensing Techniques; D004247:DNA / Q000032:analysis*; D008297:Male
D004247:DNA