Metabolism of glycerol 3-phosphate by mature boar spermatozoa.
Under anaerobic conditions boar spermatozoa metabolized fructose and glucose to lactate but did not produce ATP to the extent of that produced under aerobic conditions; the ketogenic amino acids leucine, tryptophan, phenylalanine and tyrosine were not oxidatively metabolized. Glycerol 3-phosphate was metabolized rapidly in the presence or absence of the glycolytic inhibitor, 3-chloro-1-hydroxypropanone (CHOP). In the absence of CHOP, glycerol 3-phosphate was converted to CO2, lactate, glucose 6-phosphate and fructose 6-phosphate, and ATP was produced. In the presence of CHOP, glycerol 3-phosphate did not produce CO2, lactate or ATP, but formed fructose 1,6-bisphosphate and dihydroxyacetone phosphate. With dihydroxyacetone phosphate as substrate, fructose 1,6-bisphosphate, lactate, glucose 6-phosphate, fructose 6-phosphate and ATP were produced. Accumulation of glucose 6-phosphate and fructose 6-phosphate from glycerol 3-phosphate appeared to depend on the production of ATP; if ATP was not produced, dihydroxyacetone phosphate and fructose 1,6-bisphosphate accumulated. The conversion of glycerol 3-phosphate to glycolytic intermediates appeared to be a mechanism for the conversion of substrates for the ultimate production of lactate.
Jones
A R
AR
Department of Biochemistry, University of Sydney, NSW, Australia.
Gillan
L
L
D000096:Acetone / Q000031:analogs & derivatives / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002245:Carbon Dioxide / Q000378:metabolism; D004099:Dihydroxyacetone Phosphate / Q000378:metabolism; D005636:Fructosephosphates / Q000378:metabolism; D019298:Glucose-6-Phosphate; D005958:Glucosephosphates / Q000378:metabolism; D005994:Glycerophosphates / Q000378:metabolism*; D006019:Glycolysis / Q000187:drug effects; D007773:Lactates / Q000378:metabolism; D019344:Lactic Acid; D008297:Male; D013094:Spermatozoa / Q000378:metabolism*; D013552:Swine / Q000378:metabolism*
D005636:Fructosephosphates; D005958:Glucosephosphates; D005994:Glycerophosphates; D007773:Lactates; D000096:Acetone; D002245:Carbon Dioxide; D019344:Lactic Acid; D019298:Glucose-6-Phosphate; D004099:Dihydroxyacetone Phosphate; C027618:fructose-6-phosphate; D000255:Adenosine Triphosphate; C029620:alpha-glycerophosphoric acid; C027160:1-hydroxy-3-chloroacetone