title: [The use of a new strain of Vibrio cholerae O139 as a producer of an enteric chemical vaccine].
abstract: Conditions for the submerged cultivation of a strain of V. cholerae O139, were worked out. These conditions ensured a high yield of biomass, soluble O-antigen and exoenzymes (proteinase, phospholipase A) into the culture medium, which exceeded their production by strains of serovar O1, respectively, 2, 3, 4 and 8 times. The preparation, isolated from the culture fluid and lyophilized, contained up to 50% of O-antigen and exoenzymes. In experiments on white mice the preparation exhibited low toxicity (LD50 was equal, on the average, to 1.2 mg) and immunogenicity (ED50 was equal to 3-5 micrograms) with respect to V. cholerae O139, which corresponded to the protective potency of commercial vaccine against V. cholerae O1 infection.
authors.forename: M N
authors.initials: MN
authors.lastname: Dzhaparidze
authors.forename: A V
authors.initials: AV
authors.lastname: Naumov
authors.forename: O V
authors.initials: OV
authors.lastname: Gromova
authors.forename: A K
authors.initials: AK
authors.lastname: Adamov
authors.forename: Iu Iu
authors.initials: IuIu
authors.lastname: Eliseev
authors.forename: O M
authors.initials: OM
authors.lastname: Kosmoenko
authors.forename: I A
authors.initials: IA
authors.lastname: Kuz'michenko
authors.forename: V N
authors.initials: VN
authors.lastname: Kopyrzov
authors.forename: V I
authors.initials: VI
authors.lastname: Zavorotnykh
authors.forename: T L
authors.initials: TL
authors.lastname: Zakharova
authors.forename: G V
authors.initials: GV
authors.lastname: Chekhovskaia
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology / Q000302:isolation & purification / Q000633:toxicity; D001428:Bacterial Vaccines / Q000096:biosynthesis* / Q000276:immunology / Q000302:isolation & purification / Q000633:toxicity; D003470:Culture Media; D000939:Epitopes / Q000276:immunology / Q000302:isolation & purification / Q000633:toxicity; D007114:Immunization; D007928:Lethal Dose 50; D051379:Mice; D011817:Rabbits; D014614:Vaccines, Synthetic / Q000096:biosynthesis* / Q000276:immunology / Q000302:isolation & purification / Q000633:toxicity; D014734:Vibrio cholerae / Q000254:growth & development / Q000276:immunology*
chemical_list: D000942:Antigens, Bacterial; D001428:Bacterial Vaccines; D003470:Culture Media; D000939:Epitopes; D014614:Vaccines, Synthetic
vernacular_title: Ispol'zovanie novogo shtamma Vibrio cholerae O139 v kachestve produtsenta énteral'noĭ khimicheskoĭ vaktsiny.