title: Functional correlation of NMDA receptor epsilon subunits expression with the properties of single-channel and synaptic currents in the developing cerebellum.
abstract: NMDA receptor (NMDAR) subunits epsilon 1-epsilon 4 are expressed differentially with respect to brain region and ontogenic period, but their functional roles still are unclear. We have compared an epsilon 1 subunit-ablated mutant mouse with the wild-type to characterize the effect of epsilon subunit expression on NMDAR-mediated single-channel currents and synaptic currents of granule cells in cerebellar slices. Single-channel and Western blot analyses indicated that the epsilon 2 subunit disappeared gradually during the first postnatal month in both wild-type and mutant mice. Concomitantly, the voltage-dependent Mg2+ block of NMDAR-mediated EPSCs (NMDA-EPSCs) was decreased. Throughout the developmental period studied, postnatal day 7-24 (P7-P24), the decay time course of NMDA-EPSCs in epsilon 1 mutant (-/-) mice was slower than in wild-type mice. We suggest that the expression of the epsilon 3 subunit late in development is responsible for a reduction in the sensitivity of NMDA-EPSCs to block by extracellular Mg2+ and that receptors containing the epsilon 1 subunit determine the fast kinetics of the NMDA-EPSCs.
authors.affiliation: Department of Neurophysiology, Institute for Brain Research, University of Tokyo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Takahashi
authors.forename: D
authors.initials: D
authors.lastname: Feldmeyer
authors.forename: N
authors.initials: N
authors.lastname: Suzuki
authors.forename: K
authors.initials: K
authors.lastname: Onodera
authors.forename: S G
authors.initials: SG
authors.lastname: Cull-Candy
authors.forename: K
authors.initials: K
authors.lastname: Sakimura
authors.forename: M
authors.initials: M
authors.lastname: Mishina
mesh_terms: D017677:Age Distribution; D000818:Animals; D000831:Animals, Newborn; D002531:Cerebellum / Q000502:physiology*; D008274:Magnesium / Q000494:pharmacology; D051379:Mice; D008817:Mice, Mutant Strains; D018408:Patch-Clamp Techniques; D017729:Presynaptic Terminals / Q000502:physiology*; D016194:Receptors, N-Methyl-D-Aspartate / Q000502:physiology*
chemical_list: D016194:Receptors, N-Methyl-D-Aspartate; D008274:Magnesium