The role of calcium channel entry blocker in experimental ischemia-reperfusion-induced intestinal injury.
The effect of verapamil on mesenteric ischemia reperfusion trauma was evaluated. Ischemia was performed with clamping a mesenterica superior for a period of 15 min. In 55 rats in histopathologically investigated group, the abdomen was opened 4 h after from ischemia and intestinal biopsies were performed. 55 rats were classified into 5 groups. In the first group 13 rats were chosen as control, in the second group 11 rats were chosen as SHAM control. Only midline incision was made for these animals without superior mesenteric artery clamping; in the third group (n = 10) intravenous verapamil (0.3 mg/kg) was given 15 min before ischemia; in the group 4 (n = 10) 10 min after ischemia; in the group 5 (n = 11) 10 min after reperfusion. There was statistically significant difference between group 2 and group 1 in injury severity (p < 0.05). Similarly, the injury severity of group 3 was statistically significant as compared to group 1 (p < 0.05). There was no significant change in severity of injury in group 4 and group 5 as compared to group 1 (p > 0.05). We concluded that verapamil was protective when administered during the ischemic period but did not exert this action if administered after established ischemia.
Mocan
H
H
Department of Pediatrics, Karadeniz Technical University, Trabzon, Turkey.
Gedik
Y
Y
Erduran
E
E
Mocan
Z M
ZM
Okten
A
A
Gacar
N
N
Pul
N
N
D000818:Animals; D002121:Calcium Channel Blockers / Q000627:therapeutic use*; D003250:Constriction; D007422:Intestines / Q000098:blood supply*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000209:etiology / Q000517:prevention & control*; D014700:Verapamil / Q000627:therapeutic use*
D002121:Calcium Channel Blockers; D014700:Verapamil