Northern analysis of highly folded goat alpha S1 casein mRNA.
Northern blotting using glyoxal to denature a highly folded mRNA, such as goat alpha S1-Casein E, can lead to the detection of multiple incompletely denatured forms. Formaldehyde appears to be the most suitable agent for Northern blotting due to its effective denaturing capacity and lower toxicity than methylmercuric hydroxide.
Jansà Pérez
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Departament de Patologia i Producció Animals, Facultat de Veterinària, Universitat Autònoma de Barcelona, Bellaterra, Spain. Marta@guara.uab.es
Leroux
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Sànchez Bonastre
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A
Martin
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P
D000818:Animals; D015152:Blotting, Northern / Q000379:methods*; D002364:Caseins / Q000235:genetics*; D005557:Formaldehyde / Q000494:pharmacology; D006037:Glyoxal / Q000494:pharmacology*; D006041:Goats; D008767:Methylmercury Compounds / Q000494:pharmacology; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D012333:RNA, Messenger / Q000032:analysis*
D002364:Caseins; D008767:Methylmercury Compounds; D012333:RNA, Messenger; D005557:Formaldehyde; D006037:Glyoxal; C020771:methylmercury hydroxide