NF-kappa B is induced in the nuclei of cultured rat aortic smooth muscle cells by stimulation of various growth factors.
We investigated whether induction of transcription factor NF-kappa B is involved in the proliferation of cultured rat aortic smooth muscle cell using electrophoretic mobility shift assay and immunocytochemistry. NF-kappa B was induced in the nucleus in a dose-dependent manner when the smooth muscle cells were stimulated by various growth factors such as PDGF-BB, bFGF, EGF and IGF-1, but not growth inhibitors such as TGF-beta and IFN-gamma. Among growth factors, PDGF-BB and bFGF, more potent growth stimulators, induced higher kappa B binding activity than EGF or IGF-1. These evidences were also supported by the results obtained with immunocytochemistry. Immunocytochemistry also showed that the induced NF-kappa B contained p50 and p65. These results suggest that NF-kappa B induction may be involved in the proliferation of vascular smooth muscle cell.
Obata
H
H
First Department of Internal Medicine, Faculty of Medicine, Kagoshima University, Japan.
Biro
S
S
Arima
N
N
Kaieda
H
H
Kihara
T
T
Eto
H
H
Miyata
M
M
Tanaka
H
H
D000818:Animals; D001011:Aorta; D001483:Base Sequence; D002455:Cell Division / Q000187:drug effects; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism*; D002478:Cells, Cultured; D017931:DNA Primers; D015342:DNA Probes; D004742:Enhancer Elements, Genetic; D006133:Growth Substances / Q000494:pharmacology*; D006801:Humans; D008969:Molecular Sequence Data; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D016328:NF-kappa B / Q000096:biosynthesis*; D009838:Oligodeoxyribonucleotides; D051381:Rats; D015375:Receptors, Interleukin-2 / Q000096:biosynthesis
D017931:DNA Primers; D015342:DNA Probes; D006133:Growth Substances; D016328:NF-kappa B; D009838:Oligodeoxyribonucleotides; D015375:Receptors, Interleukin-2