[Determination of free d-biotin--comparison of instrumental and non-instrumental methods of analysis].
Simple methods for determining free d-biotin were developed based on the latex agglutination reaction inhibition and competitive ELISA. Biotin-binding protein Str used as a biospecific marker was immobilized on the surface of colored polyacrolein latex particles or a nitrocellulose membrane. The sensitivity of both methods of the free d-biotin assay, which take 1.5 h, is 1.0-3.0 ng/ml.
Pavlova
I S
IS
Liubavina
I A
IA
Lukin
Iu V
IuV
Tsibul'skaia
M I
MI
Korolev
P N
PN
D000372:Agglutination Tests; D001426:Bacterial Proteins / Q000378:metabolism; D015415:Biomarkers; D001710:Biotin / Q000032:analysis* / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D012680:Sensitivity and Specificity; D019809:Streptavidin
D001426:Bacterial Proteins; D015415:Biomarkers; D001710:Biotin; D019809:Streptavidin
Opredelenie svobodnogo d-biotina--sravnenie instumental'nogo i neinstrumental'nogo metodov analiza.