External stenting reduces early medial and neointimal thickening in a pig model of arteriovenous bypass grafting.
The long-term clinical success of coronary artery bypass grafting with autologous saphenous vein is limited by progressive medial and neointimal thickening in the graft and superimposed atherosclerosis. We sought to reduce wall thickening by applying an external stent to experimental grafts in pigs. The diameter of the stent was designed to allow unrestricted initial expansion of the vein in response to arterial pressure and the stent material was highly porous so as to minimize adventitial [correction of advential] disruption. Four weeks after graft implantation, stented grafts had a larger lumen (11.2 +/- 6.2 [standard deviation] mm2 versus 7.6 +/- 3.4 mm2, p < 0.05, n = 9) and an almost fourfold thinner media (0.14 +/- 0.08 versus 0.49 +/- 0.22, p < 0.001) and neointima (0.10 +/- 0.07 versus 0.35 +/- 0.24, p < 0.001) than paired unstented grafts in the same animals. Cell proliferation was also greatly reduced by stenting in the neointimal and medial layers. The stenting procedure devised here is readily applicable to clinical coronary bypass grafts.
Angelini
G D
GD
Bristol Heart Institute, University of Bristol, United Kingdom.
Izzat
M B
MB
Bryan
A J
AJ
Newby
A C
AC
D000818:Animals; D002455:Cell Division; D001026:Coronary Artery Bypass / Q000379:methods*; D004195:Disease Models, Animal; D004730:Endothelium, Vascular / Q000737:chemistry / Q000473:pathology*; D004867:Equipment Design; D007150:Immunohistochemistry; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D019736:Prostheses and Implants; D012501:Saphenous Vein / Q000473:pathology / Q000637:transplantation*; D015607:Stents; D013552:Swine
D018809:Proliferating Cell Nuclear Antigen