The factors affecting transformation efficiency of coryneform bacteria by electroporation.
High-voltage electroporation was performed to transfer plasmid DNA of pXZ10145 into different kinds of corynebacteria strains. A number of factors that affected transformation efficiency were investigated. Cells grown in the presence of 4% glycine and harvested in the early exponential growth phase (OD600 was about 0.2) were much more easily transformed. Transformation efficiency up to 8 x 10(6) transformants per microgram of plasmid DNA with homologously derived DNA was obtained. If heterologously derived DNA was used, transformation efficiency was 10(2) to 10(3) times lower than the former one.
Na
S
S
Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.
Shen
T
T
Xiao
W
W
Jia
P
P
D000192:Actinomycetales / Q000235:genetics* / Q000254:growth & development; D002455:Cell Division / Q000187:drug effects; D004269:DNA, Bacterial / Q000378:metabolism; D004553:Electric Conductivity; D018274:Electroporation / Q000379:methods*; D005998:Glycine / Q000494:pharmacology; D014169:Transformation, Bacterial
D004269:DNA, Bacterial; D005998:Glycine