Role of leukotriene C4 in follicle-stimulating hormone (FSH) secretion in female rat pituitary.
Leukotriene C4, at doses of 0.01 and 0.1 nmol/l added to superfused cells in pulse of 4-min duration, evoked follicle-stimulating hormone (FSH) release up to 12- to 26-fold of basal secretion. Higher and lower concentrations of leukotriene C4 were not able to induce FSH secretion. Gonadotropin-releasing hormone (GnRH)-induced FSH release was reduced by 38-57% by the leukotriene receptor antagonist FPL 55712 (10 mumol/l). Moreover, we have shown that FSH release occurs parallel to leukotriene C4 synthesis in rat anterior pituitary cells. Mellitin (100 nmol/l), an activator of phospholipase A2, induced FSH and radioactivity secretion in rat anterior pituitary cells previously preincubated for 24 h with [3H]arachidonic acid (AA).
Przylipiak
A
A
Department of Obstetrics and Gynaecology, University Women's Hospital, Heidelberg, Germany.
Rabe
T
T
Kiesel
L
L
Przylipiak
J
J
Klinga
K
K
Runnebaum
B
B
D000818:Animals; D002478:Cells, Cultured; D002867:Chromones / Q000494:pharmacology; D005260:Female; D005640:Follicle Stimulating Hormone / Q000096:biosynthesis / Q000378:metabolism*; D007987:Gonadotropin-Releasing Hormone / Q000494:pharmacology; D017997:Leukotriene C4 / Q000096:biosynthesis / Q000502:physiology*; D016859:Lipoxygenase Inhibitors / Q000494:pharmacology; D008555:Melitten / Q000494:pharmacology; D010903:Pituitary Gland, Anterior / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D011863:Radioimmunoassay; D051381:Rats; D017207:Rats, Sprague-Dawley
D002867:Chromones; D016859:Lipoxygenase Inhibitors; D008555:Melitten; D017997:Leukotriene C4; D007987:Gonadotropin-Releasing Hormone; C006312:FPL 55712; D005640:Follicle Stimulating Hormone