Experimental assessment of the sensitizing properties of formaldehyde.
Formaldehyde causes upper respiratory tract irritation and has been reported in some investigations to be a cause of occupational allergic asthma. The data are equivocal, however, and it has proved difficult to confirm that exposure to formaldehyde induces respiratory sensitization or provokes the production of specific immunoglobulin E (IgE) antibody. In this study the sensitizing properties of formaldehyde were examined experimentally. This chemical elicited strong positive responses in three independent methods for the prospective identification of contact sensitizing chemicals-the guinea pig maximization test, the occluded patch test of Buehler and the murine local lymph node assay. In contrast, in a novel predictive test method for assessment of respiratory sensitization potential-the mouse IgE test-formaldehyde at the same test concentrations was negative. Furthermore, formaldehyde induced in mice a pattern of cytokine secretion by draining lymph node cells inconsistent with the stimulation of IgE antibody responses or respiratory sensitization. These data indicate that, although formaldehyde is a potent contact allergen, it lacks a significant potential to cause sensitization of the respiratory tract.
Hilton
J
J
Zeneca Central Toxicology Laboratory, Alderley Park, Macclesfield, Cheshire, UK.
Dearman
R J
RJ
Basketter
D A
DA
Scholes
E W
EW
Kimber
I
I
D000287:Administration, Topical; D000485:Allergens / Q000633:toxicity*; D000818:Animals; D016207:Cytokines / Q000187:drug effects / Q000378:metabolism; D017449:Dermatitis, Allergic Contact / Q000209:etiology* / Q000276:immunology; D004137:Dinitrochlorobenzene / Q000633:toxicity; D004305:Dose-Response Relationship, Drug; D005260:Female; D005557:Formaldehyde / Q000008:administration & dosage / Q000633:toxicity*; D006168:Guinea Pigs; D007073:Immunoglobulin E / Q000187:drug effects; D007371:Interferon-gamma / Q000096:biosynthesis / Q000187:drug effects; D016753:Interleukin-10 / Q000096:biosynthesis; D007509:Irritants / Q000633:toxicity; D008198:Lymph Nodes / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D010328:Patch Tests / Q000379:methods; D010796:Phthalic Anhydrides / Q000633:toxicity
D000485:Allergens; D016207:Cytokines; D004137:Dinitrochlorobenzene; D007509:Irritants; D010796:Phthalic Anhydrides; D016753:Interleukin-10; D005557:Formaldehyde; D007073:Immunoglobulin E; C015559:trimellitic anhydride; D007371:Interferon-gamma