title: Elucidation of antioxidant activity of dihydrolipoic acid toward hydroxyl radical using a novel hydroxyl radical generator NP-III.
abstract: The photosensitive organic hydroperoxide, NP-III, which produces hydroxyl radicals on illumination by UVA light, was used to examine the antioxidant activity of dihydrolipoic acid toward hydroxyl radical. Apolipoprotein (apo-B) of human low density lipoprotein (LDL), and bovine serum albumin (BSA), were irradiated with UVA in the presence of NP-III and dihydrolipoic acid. The oxidation of BSA and apo-B of LDL by NP-III was completely inhibited by dihydrolipoic acid. ESR studies using dimethylpyrroline oxide (DMPO) as a spin trapping reagent also revealed that in the presence of dihydrolipoic acid, the DMPO-OH adduct produced from the irradiation of NP-III and DMPO completely disappeared. Hence, the scavenging activity of dihydrolipoic acid is not due to its chelating activity toward transition metals (ferrous ions). The results lead us to conclude that dihydrolipoic acid is an efficient hydroxyl radical scavenger through the direct reaction of dihydrolipoic acid with hydroxyl radical.
authors.affiliation: Department of Molecular and Cell Biology, University of California at Berkeley 94720-3200, USA.
authors.forename: S
authors.initials: S
authors.lastname: Matsugo
authors.forename: L J
authors.initials: LJ
authors.lastname: Yan
authors.forename: D
authors.initials: D
authors.lastname: Han
authors.forename: H J
authors.initials: HJ
authors.lastname: Tritschler
authors.forename: L
authors.initials: L
authors.lastname: Packer
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D001055:Apolipoproteins B / Q000737:chemistry*; D003497:Cyclic N-Oxides; D004578:Electron Spin Resonance Spectroscopy; D016166:Free Radical Scavengers / Q000494:pharmacology; D010084:Oxidation-Reduction; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D019097:Spin Trapping; D008063:Thioctic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D014466:Ultraviolet Rays
chemical_list: D000975:Antioxidants; D001055:Apolipoproteins B; D003497:Cyclic N-Oxides; D016166:Free Radical Scavengers; D012710:Serum Albumin, Bovine; C017245:5,5-dimethyl-1-pyrroline-1-oxide; D008063:Thioctic Acid; C015144:dihydrolipoic acid