title: Secondary structure and tertiary fold of the birch pollen allergen Bet v 1 in solution.
abstract: Bet v 1 is the major birch pollen allergen and therefore the main cause of type I allergies observed in early spring. It is composed of 159 amino acid residues adding up to a molecular mass of 17 kDa. We determined the secondary structure and tertiary fold of full-length Bet v 1 by NMR spectroscopy. Two- and three-dimensional NMR measurements suggest that Bet v 1 is a globular monomer in solution with a high content of well defined secondary structure. Of the total of 159 residues, 135 could be sequentially assigned, using an improved assignment strategy based mainly on heteronuclear experiments. An improved strategy for structure calculation revealed three helices and two beta-sheets as major elements of secondary structure. The globular tertiary structure is mainly stabilized by two antiparallel beta-sheets. The two helices at the C terminus are in accordance with the results from the solution structure of the chemically synthesized peptide Bet v 1-(125-154). This peptide is composed of two helices connected by a hinge. The structural features of Bet v 1 are highly similar to those found in the Ambrosia allergen Amb t V.
authors.affiliation: Lehrstuhl für Biopolymere, Universität Bayreuth, Universitätsstr. 30, D-95447 Bayreuth, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Faber
authors.forename: A
authors.initials: A
authors.lastname: Lindemann
authors.forename: H
authors.initials: H
authors.lastname: Sticht
authors.forename: A
authors.initials: A
authors.lastname: Ejchart
authors.forename: A
authors.initials: A
authors.lastname: Kungl
authors.forename: M
authors.initials: M
authors.lastname: Susani
authors.forename: R W
authors.initials: RW
authors.lastname: Frank
authors.forename: D
authors.initials: D
authors.lastname: Kraft
authors.forename: M
authors.initials: M
authors.lastname: Breitenbach
authors.forename: P
authors.initials: P
authors.lastname: Rösch
mesh_terms: D000485:Allergens / Q000737:chemistry* / Q000235:genetics; D000595:Amino Acid Sequence; D052179:Antigens, Plant; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry* / Q000235:genetics; D017510:Protein Folding; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D012996:Solutions
chemical_list: D000485:Allergens; D052179:Antigens, Plant; D010940:Plant Proteins; D012996:Solutions; C061312:Bet v 1 allergen, Betula