title: Effects of three inhibitors of nitric oxide synthase on host resistance to bacterial infection.
abstract: OBJECTIVE
  To examine the effects of three nitric oxide synthase inhibitors on survival in a murine sepsis model.
  
  
  METHODS
  Prospective randomized experimental trials.
  
  
  METHODS
  Laboratory.
  
  
  METHODS
  Female Balb/c mice.
  
  
  METHODS
  Escherichia coli (10(8) colony-forming-units/body) were injected into the peritoneal cavities of Balb/c mice. NG-nitro-L-arginine-methyl-ester, NG-monomethyl-L-arginine, or N-iminoethyl-L-ornithine was given at various concentrations, intraperitoneally, one hour before bacterial challenge.
  
  
  METHODS
  One hundred and fifteen animals were observed for survival.
  
  
  RESULTS
  These inhibitors provided the mice no protection from the bacterial challenge. Notably, pretreatment with NG-nitro-L-arginine-methyl-ester (100 mg/kg i.p.) actually reduced survival time after E. coli challenge.
  
  
  CONCLUSIONS
  Inhibition of nitric oxide production improved neither the survival time nor rate in this murine sepsis model.
authors.affiliation: Department of Surgery, University of Tokyo, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Fukatsu
authors.forename: H
authors.initials: H
authors.lastname: Saito
authors.forename: R
authors.initials: R
authors.lastname: Fukushima
authors.forename: M T
authors.initials: MT
authors.lastname: Lin
authors.forename: T
authors.initials: T
authors.lastname: Inoue
authors.forename: T
authors.initials: T
authors.lastname: Inaba
authors.forename: S
authors.initials: S
authors.lastname: Furukawa
authors.forename: I
authors.initials: I
authors.lastname: Han
authors.forename: T
authors.initials: T
authors.lastname: Muto
mesh_terms: D000818:Animals; D001120:Arginine / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000494:pharmacology / Q000627:therapeutic use; D004195:Disease Models, Animal; D004791:Enzyme Inhibitors / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D004927:Escherichia coli Infections / Q000276:immunology* / Q000401:mortality / Q000517:prevention & control; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D019331:NG-Nitroarginine Methyl Ester; D009569:Nitric Oxide / Q000096:biosynthesis; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors*; D009952:Ornithine / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000494:pharmacology / Q000627:therapeutic use; D011446:Prospective Studies; D011897:Random Allocation; D019323:omega-N-Methylarginine
chemical_list: D004791:Enzyme Inhibitors; D019323:omega-N-Methylarginine; D009569:Nitric Oxide; C065027:N(G)-iminoethylornithine; D001120:Arginine; D009952:Ornithine; D019001:Nitric Oxide Synthase; D019331:NG-Nitroarginine Methyl Ester