title: [Specific serologic studies with a novel authentic HPV antigen (virus-like particles) for HPV-6 antibodies in gynecologic patient samples].
abstract: OBJECTIVE
  A serological assay for genital HPV infection would provide important additional information to HPV DNA diagnostic methods, since it would evaluate prior exposure to the viruses, detect significant systemic immunologic response to virus infection, and could be performed in most clinical laboratories.
  
  
  METHODS
  Serum samples from three groups of patients attending a gynecology clinic were analysed by direct ELISA for specific IgG antibodies to baculovirus-expressed HPV-6 and BPV-1-L1-VLPs.
  
  
  RESULTS
  Positive IgG reactivity to HPV-6-L1-VLPs were 4/72 (6%) in a control group, 28/73 (38%) in a condyloma group and 17/62 (17%) in cervical intraepithelial neoplasia patients. Individual IgG ELISA values of condyloma and CIN patients for HPV-6-L1-VLPs demonstrated no correlation to results with BPV-1-L1-VLPs.
  
  
  CONCLUSIONS
  These data show that HPV-6-L1-VLPs are effective antigens for serological studies and can detect species specific antibodies with important implications for diagnosis, epidemiology, insights to natural course of disease, prognosis and evaluation of vaccination.
authors.affiliation: Universitätsklinik für Frauenheilkunde, Innsbruck.
authors.forename: K
authors.initials: K
authors.lastname: Heim
authors.forename: N D
authors.initials: ND
authors.lastname: Christensen
authors.forename: R
authors.initials: R
authors.lastname: Höpfl
authors.forename: B
authors.initials: B
authors.lastname: Wartusch
authors.forename: A
authors.initials: A
authors.lastname: Zeimet
authors.forename: C
authors.initials: C
authors.lastname: Larcher
authors.forename: N
authors.initials: N
authors.lastname: Ruth
authors.forename: A
authors.initials: A
authors.lastname: Bergant
authors.forename: G
authors.initials: G
authors.lastname: Pirschner
authors.forename: M P
authors.initials: MP
authors.lastname: Dierich
mesh_terms: D000293:Adolescent; D000328:Adult; D000914:Antibodies, Viral / Q000097:blood*; D000918:Antibody Specificity / Q000276:immunology; D002213:Capsid / Q000276:immunology; D036022:Capsid Proteins; D003218:Condylomata Acuminata / Q000175:diagnosis / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005833:Genital Neoplasms, Female / Q000175:diagnosis* / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D008875:Middle Aged; D009856:Oncogene Proteins, Viral / Q000276:immunology*; D027383:Papillomaviridae / Q000276:immunology*; D030361:Papillomavirus Infections / Q000175:diagnosis* / Q000276:immunology; D014412:Tumor Virus Infections / Q000175:diagnosis* / Q000276:immunology; D014764:Viral Proteins
chemical_list: D000914:Antibodies, Viral; D036022:Capsid Proteins; D007074:Immunoglobulin G; C085217:L1 protein, Bovine papillomavirus; D009856:Oncogene Proteins, Viral; D014764:Viral Proteins; C091560:L1 protein, Human papillomavirus type 6
vernacular_title: Spezifische serologische Untersuchungen mit einem neuartigen authentischen HPV-Antigen (Virus-like Particles) auf HPV-6 Antikörper bei gynäkologischen Patientenkollektiven.