[An additional test in the identification of Enterobacteriaceae and some representatives of the genus Vibrio].
An additional test: aerobic redox fermentation in semiliquid Hiss' medium with mannitol is recommended for the indication of Enterobacteriaceae, Vibrio, etc. The majority of mannite-fermenting enterobacteria change the color of the indicator in a thin upper layer of a column of semiliquid Hiss' medium in comparison with the bulk of medium after 20-24 h growth in it. Such changes of the indicator are never observed with Shigella, Salmonella, Vibrio bacteria of the studied strains, Yersinia, some cocci, etc., which may be considered as an additional differential diagnostic test at early stages of investigation with due consideration for other known signs. The aerobic redox test does not require additional quantities of nutrient media, reagents, or glassware.
Bril'man
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IaE
D001424:Bacterial Infections / Q000175:diagnosis; D001431:Bacteriological Techniques; D002954:Citrobacter / Q000302:isolation & purification; D003470:Culture Media; D003937:Diagnosis, Differential; D004754:Enterobacter / Q000302:isolation & purification; D004755:Enterobacteriaceae / Q000302:isolation & purification*; D004926:Escherichia coli / Q000302:isolation & purification; D006801:Humans; D007202:Indicators and Reagents; D008353:Mannitol; D010084:Oxidation-Reduction; D012706:Serratia marcescens / Q000302:isolation & purification; D012760:Shigella / Q000302:isolation & purification; D014733:Vibrio / Q000302:isolation & purification*; D015008:Yersinia enterocolitica / Q000302:isolation & purification
D003470:Culture Media; D007202:Indicators and Reagents; D008353:Mannitol
Dopolnitel'nyĭ test dlia identifikatsii bakteriĭ semeĭstva Enterobacteriaceae i nekotorykh predstaviteleiĭ roda Vibrio.