Collagen synthesis and cell growth in chick embryo fibroblasts: influence of colchicine, cytochalasin B and concanavalin A.
Culturing of chick embryo fibroblasts in the presence of colchicine or cytochalasin B with and without concanavalin A (Con A) demonstrated that colchicine induces greater neosynthesis of endocellular type I collagen, whereas cytochalasin B boosts secretion. The effects are modified by the addition of Con A, which increases alpha 2 more than a1 chain production. 3H-thymidine incorporation is unaffected by cytochalasin B, but stimulated by colchicine. Con A neutralizes the stimulatory action of colchicine. It would therefore seem that Con A exerts transmembrane control of effects induced by colchicine and cytochalasin B by binding to cell surface receptors and so triggering rearrangement of the cytoskeleton.
Bodo
M
M
Istituto di Istologia ed Embriologia generale, Università di Ferrara, Italia.
Carinci
P
P
Baroni
T
T
Becchetti
E
E
Bellucci
C
C
Pezzetti
F
F
Giammarioli
M
M
Stabellini
G
G
Arena
N
N
D000199:Actins / Q000037:antagonists & inhibitors; D000818:Animals; D002455:Cell Division / Q000187:drug effects / Q000502:physiology; D002642:Chick Embryo; D003078:Colchicine / Q000494:pharmacology*; D003094:Collagen / Q000096:biosynthesis* / Q000187:drug effects; D003208:Concanavalin A / Q000494:pharmacology*; D003571:Cytochalasin B / Q000494:pharmacology*; D003599:Cytoskeleton / Q000187:drug effects / Q000502:physiology; D004247:DNA / Q000096:biosynthesis; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D005456:Fluorescent Dyes; D013936:Thymidine / Q000378:metabolism; D014316:Tritium / Q000378:metabolism; D050257:Tubulin Modulators
D000199:Actins; D005456:Fluorescent Dyes; D050257:Tubulin Modulators; D014316:Tritium; D003208:Concanavalin A; D003571:Cytochalasin B; D003094:Collagen; D004247:DNA; D003078:Colchicine; D013936:Thymidine