title: The protein fold of the hyaluronate-binding proteoglycan tandem repeat domain of link protein, aggrecan and CD44 is similar to that of the C-type lectin superfamily.
abstract: Link protein and aggrecan of the extracellular matrix each contain two proteoglycan tandem repeat (PTR) domains that interact with hyaluronate. Consensus secondary structure predictions for 59 PTR sequences and 129 C-type lectin sequences give similar patterns of two alpha-helices and up to seven beta-strands. Protein fold recognition analyses show that the 59 PTR sequences are highly compatible with the C-type lectin crystal structure. The predicted fold consists of a conserved motif formed from an antiparallel beta-sheet flanked by two alpha-helices, the motif being attached to two distinct types of beta-sheet region in the two superfamilies. Arg9 or Lys11 on an exposed loop and up to three other Arg residues in the beta-sheet region are conserved and may form part of a hyaluronate binding site.
authors.affiliation: Department of Biochemistry and Molecular Biology, Royal Free Hospital School of Medicine, London, UK.
authors.forename: N C
authors.initials: NC
authors.lastname: Brissett
authors.forename: S J
authors.initials: SJ
authors.lastname: Perkins
mesh_terms: D053674:Aggrecans; D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D017124:Conserved Sequence; D016326:Extracellular Matrix Proteins / Q000737:chemistry*; D006801:Humans; D018960:Hyaluronan Receptors / Q000737:chemistry*; D006820:Hyaluronic Acid / Q000737:chemistry*; D037102:Lectins; D037181:Lectins, C-Type; D008958:Models, Molecular; D008969:Molecular Sequence Data; D017510:Protein Folding; D017433:Protein Structure, Secondary; D011506:Proteins / Q000737:chemistry*; D011509:Proteoglycans / Q000737:chemistry*; D017386:Sequence Homology, Amino Acid; D000099249:Proteoglycan Link Protein
chemical_list: D053674:Aggrecans; D016326:Extracellular Matrix Proteins; D018960:Hyaluronan Receptors; D006820:Hyaluronic Acid; D037102:Lectins; D037181:Lectins, C-Type; D011506:Proteins; D011509:Proteoglycans; D000099249:Proteoglycan Link Protein