Dietary fat influences Ia antigen expression, cytokines and prostaglandin E2 production of immune cells in autoimmune-prone NZB x NZW F1 mice.
To elucidate further the influences of dietary fat on autoimmune diseases, two groups of NZB/W F1 mice were fed with diets containing 200 g dietary fat/kg and 50 g dietary fat/kg (control) respectively. The difference in energy intake between these two groups was compensated with carbohydrate. Mice were bled regularly every month and some of them were killed for in vitro experiments after 5 months experimental diets. Higher immunoglobulin (Ig)M and IgG anti-double stranded DNA antibody levels, shortened life span and worsened proteinuria were noted in mice fed on the high-fat diet compared with those fed on 50 g dietary fat/kg. Phenotypic analyses of spleen cells and peritoneal exudate cells showed that the percentage of CD5+ B cells and the mean fluorescent intensity of major histocompatibility molecules on the surface of both types of cells were higher in mice fed on the high-fat diet. In general, higher type 2 T-helper cell activity was noted in mice fed on the high-fat diet. In addition, cytokines such as interleukin-6, tumour necrosis factor-alpha and prostaglandin E2 (PGE2) produced by lipopolysaccharide-stimulated peritoneal exudate cells were also higher in the high-dietary-fat group. These studies suggest that high dietary fat and its related PGE2 level might have a critical effect on the frequency of CD5+ B cells, cytokine production, macrophage function and subsequent autoimmune regulation in autoimmune mice.
Lin
B F
BF
Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan, Republic of China.
Huang
C H
CH
Chiang
B L
BL
Jeng
S J
SJ
D000818:Animals; D000974:Antibodies, Antinuclear / Q000276:immunology; D015551:Autoimmunity / Q000502:physiology*; D001402:B-Lymphocytes / Q000276:immunology; D018956:CD5 Antigens / Q000276:immunology; D016207:Cytokines / Q000378:metabolism*; D004041:Dietary Fats / Q000008:administration & dosage / Q000276:immunology*; D015232:Dinoprostone / Q000096:biosynthesis*; D005260:Female; D000949:Histocompatibility Antigens Class II / Q000378:metabolism*; D015850:Interleukin-6 / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D008180:Lupus Erythematosus, Systemic / Q000276:immunology; D051379:Mice; D008817:Mice, Mutant Strains; D010537:Peritoneum / Q000378:metabolism; D013154:Spleen / Q000276:immunology; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D000974:Antibodies, Antinuclear; D018956:CD5 Antigens; D016207:Cytokines; D004041:Dietary Fats; D000949:Histocompatibility Antigens Class II; D015850:Interleukin-6; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D015232:Dinoprostone