title: Redefinition of tropism of common macrophage-tropic human immunodeficiency virus type 1.
abstract: Previous studies have suggested that the abilities of human immunodeficiency virus type 1 (HIV-1) to infect primary macrophages and transformed T cell lines are mutually exclusive and define an important biological distinction among HIV-1 strains. In a survey of eight macrophage-tropic HIV-1 strains and nine T cell lines, all frequently used in studies of tropism, we have found that six virus strains replicate in one or more T cell lines and that four T cell lines are highly susceptible to macrophage-tropic HIV-1. Passage through T cell lines did not affect the tropism or the env V3 sequence of monocytotropic HIV-1 strains. We conclude that HIV-1 replication in transformed T cells and primary macrophages are not mutually exclusive, and that as such, these definitions of tropism per se are not generally useful markers for other biological properties of HIV-1.
authors.affiliation: Molecular Virology Laboratory, Columbia University, New York, New York 10019, USA.
authors.forename: I H
authors.initials: IH
authors.lastname: Chowdhury
authors.forename: M J
authors.initials: MJ
authors.lastname: Potash
authors.forename: D J
authors.initials: DJ
authors.lastname: Volsky
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002460:Cell Line; D015752:Genes, env / Q000235:genetics; D015497:HIV-1 / Q000145:classification / Q000254:growth & development*; D006801:Humans; D008264:Macrophages / Q000821:virology*; D008969:Molecular Sequence Data; D013601:T-Lymphocytes / Q000821:virology*; D018523:Tropism / Q000235:genetics* / Q000276:immunology; D014779:Virus Replication