title: Micronucleus frequency in peripheral blood lymphocytes from patients with systemic lupus erythematosus.
abstract: In this study we evaluated the effect of immunosuppressive drug, cyclophosphamide, on lymphocytes from systemic lupus erythematosus (SLE), using the micronucleus test. Micronucleated peripheral blood lymphocytes were analyzed in cytochalasin B treated binucleated lymphocytes. There was significant increase in cells with micronuclei (P < 0.001) in SLE patients who had received cyclophosphamide (10.05 +/- 4.7) in comparison to the patients with SLE who had not received any medical treatment (6.24 +/- 2.42) and control normal subjects (5.48 +/- 2.24). The SLE patients who used cyclophosphamide showed a slightly higher frequency of micronucleated cells in the first 48 h after treatment. The present study emphasized the lack of correlation between age, sex and frequency of micronuclei.
authors.affiliation: Department of Biology and Genetics, Faculty of Medicine, Bucharest.
authors.forename: E
authors.initials: E
authors.lastname: Severin
mesh_terms: D003520:Cyclophosphamide / Q000009:adverse effects* / Q000627:therapeutic use; D005260:Female; D006801:Humans; D007166:Immunosuppressive Agents / Q000009:adverse effects* / Q000627:therapeutic use; D008180:Lupus Erythematosus, Systemic / Q000097:blood* / Q000188:drug therapy* / Q000235:genetics; D008214:Lymphocytes / Q000648:ultrastructure*; D008297:Male; D048629:Micronuclei, Chromosome-Defective / Q000187:drug effects* / Q000648:ultrastructure
chemical_list: D007166:Immunosuppressive Agents; D003520:Cyclophosphamide