title: Phosphocholine and choline content of rat sarcoma cells grown in the presence and absence of serum.
abstract: Rat sarcoma cells were grown in vitro in tissue culture medium (DMEM) supplemented with 10% foetal calf serum for 5 to 8 days followed by serum withdrawal to produce populations of cells with a variety of cell cycle distributions. Phosphocholine (PCho) and choline content and S + G2 fraction were determined. The phosphocholine content of faster growing populations of serum supplemented cells was higher than the slower growing populations. Choline content was not consistently associated with S + G2 fraction. Serum deprivation was accompanied by a decrease in S + G2 fraction after 24 hours but even after 48 hours PCho content was only slightly decreased.
authors.affiliation: Department of Nuclear Medicine, Royal Marsden Hospital, Sutton, Surrey, U.K.
authors.forename: T A
authors.initials: TA
authors.lastname: Smith
authors.forename: S
authors.initials: S
authors.lastname: Eccles
authors.forename: G
authors.initials: G
authors.lastname: Box
authors.forename: J C
authors.initials: JC
authors.lastname: Titley
authors.forename: M O
authors.initials: MO
authors.lastname: Leach
authors.forename: V R
authors.initials: VR
authors.lastname: McCready
mesh_terms: D000818:Animals; D001790:Blood Physiological Phenomena; D002794:Choline / Q000032:analysis*; D003470:Culture Media; D016195:G2 Phase; D010767:Phosphorylcholine / Q000032:analysis*; D051381:Rats; D016196:S Phase; D012513:Sarcoma, Experimental / Q000737:chemistry* / Q000473:pathology
chemical_list: D003470:Culture Media; D010767:Phosphorylcholine; D002794:Choline