Evaluation of blood clearance rates and biodistribution of poly(2-oxazoline)-grafted liposomes.
Two amphipatic polymers of the poly(2-oxazoline) family, poly(2-methyl-2-oxazoline) (PMOZ) and poly(2-ethyl-2-oxazoline) (PEOZ), were synthesized with the carboxylic group positioned at either the initiation or termination ends of the polymer chains. Distearoylphosphatidylethanolamine was covalently linked to the carboxyl groups of the polymers, resulting in conjugates which incorporate readily into liposomes. Systematic evaluation of plasma clearance kinetics and biodistribution of liposomes containing hydrogenated soy phosphatidylcholine, cholesterol, and 5 mol % the polymer-lipid conjugates in mice revealed the following. Both polymers, PMOZ and PEOZ, exhibited long plasma lifetimes and low hepatosplenic uptake. PMOZ was more effective at decreasing blood clearance rates than PEOZ. The best results, which were quantitatively comparable to the results obtained with the optimized preparations of methoxypolyethylene glycol(PEG)-2000-grafted liposomes, were obtained with formulations containing PMOZ of molecular weight 3260.
Zalipsky
S
S
SEQUUS Pharmaceuticals, Inc., Menlo Park, CA 94025, USA.
Hansen
C B
CB
Oaks
J M
JM
Allen
T M
TM
D000818:Animals; D005260:Female; D007700:Kinetics; D008081:Liposomes / Q000737:chemistry* / Q000378:metabolism*; D051379:Mice; D008815:Mice, Inbred Strains; D008970:Molecular Weight; D011108:Polymers / Q000737:chemistry / Q000378:metabolism*; D014018:Tissue Distribution
D008081:Liposomes; D011108:Polymers