title: Phospholipase D inhibitors from a Myrsine species.
abstract: The phospholipase D-inhibitory activity of a methanol extract from the leaves of a New Zealand plant, Myrsine australis, has been attributed to two new saponins 1 and 2. Compound 1 was assigned as 3-0-[-beta-D-xylopyranosyl-(1-->2)-0-beta-D-glucopyranosyl-(1-->4)- -[0-beta-D -glucopyranosyl-(1-->2)]-alpha-L-arabinosyl]-16alpha-hydroxy-+ ++13beta,28-epoxyoleanane and 2 as 3beta-0-[-beta-rhamnopyranosyl-(1-->2)-0-beta-D-glucopyranosyl-(1-->4)-[ 0-beta-D-glucopranosyl]-alpha-L-arabinopyranosyl]-16alpha  -hydroxy-13beta, 28-epoxyleanane. Compounds 1 and 2 showed IC50 values of 3 and 2 microM, respectively, versus phorbol 12-myrisate-13-acetate-stimulated phosphlipase D in human promyelocytic leukemic (HL-60) cells. Compounds 1 and 2 also inhibited fMLP (formyl-Met-Leu-Phe) stimulated phospholipase D with IC50 values of 8 and 24 microM, respectively.
authors.affiliation: Schering-Plough Research Institute, Kenilworth, New Jersey 07033, USA.
authors.forename: V R
authors.initials: VR
authors.lastname: Hegde
authors.forename: J
authors.initials: J
authors.lastname: Silver
authors.forename: M G
authors.initials: MG
authors.lastname: Patel
authors.forename: R
authors.initials: R
authors.lastname: Bryant
authors.forename: J
authors.initials: J
authors.lastname: Pai
authors.forename: P R
authors.initials: PR
authors.lastname: Das
authors.forename: M S
authors.initials: MS
authors.lastname: Puar
authors.forename: P A
authors.initials: PA
authors.lastname: Cox
mesh_terms: D002240:Carbohydrate Sequence; D002852:Chromatography, Ion Exchange; D004791:Enzyme Inhibitors / Q000302:isolation & purification* / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D010739:Phospholipase D / Q000037:antagonists & inhibitors*; D010944:Plants / Q000737:chemistry*; D012503:Saponins / Q000302:isolation & purification* / Q000494:pharmacology; D016339:Spectrometry, Mass, Fast Atom Bombardment
chemical_list: D004791:Enzyme Inhibitors; D012503:Saponins; D010739:Phospholipase D