title: Repair of the in vitro HIV-1-induced immunosuppression and blockade of the generation of functional suppressive CD8 cells by anti-alpha interferon and anti-Tat antibodies.
abstract: The acute human immunodeficiency virus type 1 (HIV-1) infection of activated peripheral blood mononuclear cells (PBMCs) from normal donors results in inhibition of cell proliferation and generation of functional suppressive T cells. Cultured HIV-1 infected PBMCs but not uninfected PBMCs, following irradiation, can inhibit the proliferation of antigen-activated autologous T cells in a dose-dependent way. CD8+ cell subpopulation is responsible for this inhibition. The presence of anti-alpha interferon (IFN alpha) and anti-Tat antibodies in the culture medium counteracts the HIV-1-induced immunosuppression and prevents the generation of suppressive T cells by these PBMCs. The reported data should have major implications for strategies of AIDS treatment which, in association with antiviral drugs, aim at targetting immune disorders.
authors.affiliation: Université Pierre et Marie Curie, Paris, France.
authors.forename: A
authors.initials: A
authors.lastname: Lachgar
authors.forename: J
authors.initials: J
authors.lastname: Bernard
authors.forename: B
authors.initials: B
authors.lastname: Bizzini
authors.forename: A
authors.initials: A
authors.lastname: Astgen
authors.forename: H
authors.initials: H
authors.lastname: Le Coq
authors.forename: M
authors.initials: M
authors.lastname: Fouchard
authors.forename: V
authors.initials: V
authors.lastname: Chams
authors.forename: M
authors.initials: M
authors.lastname: Feldman
authors.forename: A
authors.initials: A
authors.lastname: Burny
authors.forename: J F
authors.initials: JF
authors.lastname: Zagury
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000276:immunology* / Q000821:virology; D000906:Antibodies / Q000494:pharmacology; D018414:CD8-Positive T-Lymphocytes / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects; D015696:Gene Products, tat / Q000276:immunology*; D015497:HIV-1; D006801:Humans; D007108:Immune Tolerance / Q000187:drug effects; D066298:In Vitro Techniques; D016898:Interferon-alpha / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000187:drug effects; D050378:T-Lymphocytes, Regulatory / Q000187:drug effects; D054322:tat Gene Products, Human Immunodeficiency Virus
chemical_list: D000906:Antibodies; D015696:Gene Products, tat; D016898:Interferon-alpha; D054322:tat Gene Products, Human Immunodeficiency Virus