Bacterial adhesion to orthopedic implant polymers.
The degradable polymers poly(orthoester) (POE), poly(L-lactic acid) (PLA), and the nondegradable polymers polysulfone (PSF), polyethylene (PE), and poly(ether ether ketone) (PEEK) were exposed to cultures of Staphylococcus epidermidis, Pseudomonas aeruginosa, or Escherichia coli. Bacteria washed and resuspended in phosphate buffered saline (PBS) adhered to polymers in amounts nearly twice those of bacteria that were left in their growth medium, tryptic soy broth (TSB). In TSB, there was variation in adhesion from species to species, but no significant variation from polymer to polymer within one species. In PBS there were significant differences in the amounts of bacteria adhering to the various polymers with the exception, of S. epidermidis, which had similar adhesion to all polymers. As a whole, P. aeruginosa was the most adherent while S. epidermidis was the least adherent. The estimated values of the free energy of adhesion (delta Fadh) correlated with the amount of adherent P. aeruginosa. When POE, PLA, and PSF were exposed to hyaluronic acid (HA) before exposure to the bacteria, there was 50% more adhesion of E. coli and P. aeruginosa on POE and PLA. With respect to bacterial adhesion, the biodegradable polymers (POE and PLA) in general were not significantly different from the nondegradable polymers.
Barton
A J
AJ
Department of Chemical Engineering, Brigham Young University, Provo, Utah 84602, USA.
Sagers
R D
RD
Pitt
W G
WG
D001422:Bacterial Adhesion / Q000187:drug effects / Q000502:physiology*; D001672:Biocompatible Materials / Q000737:chemistry*; D002021:Buffers; D003470:Culture Media; D004926:Escherichia coli / Q000254:growth & development / Q000502:physiology; D006820:Hyaluronic Acid / Q000494:pharmacology; D009984:Orthopedic Fixation Devices; D011108:Polymers; D011550:Pseudomonas aeruginosa / Q000254:growth & development / Q000502:physiology; D017550:Spectroscopy, Fourier Transform Infrared; D013212:Staphylococcus epidermidis / Q000187:drug effects / Q000254:growth & development / Q000502:physiology; D013499:Surface Properties; D013816:Thermodynamics
D001672:Biocompatible Materials; D002021:Buffers; D003470:Culture Media; D011108:Polymers; D006820:Hyaluronic Acid