A human monoclonal antibody to carbohydrate moiety of carcinoembryonic antigen.
Hybridoma BSRF-S-97, secreting a human monoclonal antibody of IgG1 subclass reactive to the carcinoembryonic antigen, was generated by fusing the regional lymph node lymphocytes from a cervical cancer patient with RF-S1 human-mouse heteromyeloma fusion line. This monoclonal antibody was found specifically reactive to carinoembryonic antigen-producing cell lines, including those of cervical cancer (SKG-II), mucinous type ovarian cancer (RMUG-L), stomach cancer (MKN-45), and lung cancer (PC-10). The monoclonal antibody reactivity with pepsin- and periodate-treated carcinoembryonic antigen demonstrated that this monoclonal antibody recognizes the carbohydrate moiety of carcinoembryonic antigen specifically. Possibilities of the monoclonal antibody reaction with mucin and blood-group antigens were excluded by the comparative studies with a placental mucin-containing protein which reacted with carcinoembryonic antigen-specific rabbit polyclonal antibody. The monoclonal antibody conjugated with Pseudomonas exotoxin showed potent regression effects on the growth of the MKN-45 cell line in both the dish culture and xenografted nude mice, indicating potential usefulness of this human monoclonal antibody as a promising tumor targeting vehicle.
Tsukazaki
K
K
Department of Obstetrics and Gynecology, School of Medicine, Keio University, Tokyo, Japan.
Kuroda
K
K
Mochizuki
K
K
Kubushiro
K
K
Fukuchi
T
T
Kato
M
M
Hashizume
S
S
Nozawa
S
S
D000818:Animals; D000911:Antibodies, Monoclonal / Q000627:therapeutic use; D002241:Carbohydrates / Q000737:chemistry / Q000276:immunology; D002272:Carcinoembryonic Antigen / Q000737:chemistry / Q000276:immunology*; D000939:Epitopes / Q000737:chemistry; D005260:Female; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D000922:Immunotoxins / Q000627:therapeutic use; D008175:Lung Neoplasms / Q000276:immunology; D051379:Mice; D008819:Mice, Nude; D009374:Neoplasms, Experimental / Q000276:immunology / Q000628:therapy; D010051:Ovarian Neoplasms / Q000276:immunology; D011817:Rabbits; D013274:Stomach Neoplasms / Q000276:immunology; D014407:Tumor Cells, Cultured; D002583:Uterine Cervical Neoplasms / Q000276:immunology
D000911:Antibodies, Monoclonal; D002241:Carbohydrates; D002272:Carcinoembryonic Antigen; D000939:Epitopes; D000922:Immunotoxins