title: Significance of anti-nuclear matrix antibodies in patients with in vivo speckled antinuclear antibody staining.
abstract: OBJECTIVE
  Evidence suggests that patients with in vivo speckled antinuclear antibody (ANA) patterns have high titers of circulating ANA, specifically anti-U1 RNP antibody. A small percentage of patients with high titers of anti-U1 RNP antibody have anti-nuclear matrix antibodies, and some also demonstrate in vivo ANA. This study was designed to screen for the presence of anti-nuclear matrix antibodies in patients with in vivo ANA.
  
  
  METHODS
  Anti-nuclear matrix antibodies were detected by indirect immunofluorescence on HCI-extracted HEp-2 cell substrate, by enzyme-linked immunosorbent assay, and by immunoblot analysis.
  
  
  RESULTS
  All 10 patients with in vivo ANA were found to have anti-nuclear matrix antibody demonstrated using HCI-extracted HEp-2 cell substrate, and all exhibited antibody activity to a 36-kd protein from nuclear matrix antigen.
  
  
  CONCLUSIONS
  These results suggest that anti-nuclear matrix antibodies are a major factor in the development of in vivo ANA.
authors.affiliation: University of Pittsburgh, Pennsylvania, USA.
authors.forename: J S
authors.initials: JS
authors.lastname: Deng
authors.forename: L
authors.initials: L
authors.lastname: Benedict
authors.forename: D H
authors.initials: DH
authors.lastname: Deng
authors.forename: C V
authors.initials: CV
authors.lastname: Oddis
authors.forename: J
authors.initials: J
authors.lastname: Fratto
mesh_terms: D000974:Antibodies, Antinuclear / Q000032:analysis* / Q000097:blood / Q000302:isolation & purification; D000918:Antibody Specificity; D034961:Antigens, Nuclear; D001324:Autoantigens / Q000276:immunology* / Q000302:isolation & purification; D002467:Cell Nucleus / Q000737:chemistry / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D015151:Immunoblotting; D015603:Keratinocytes / Q000737:chemistry / Q000276:immunology; D009687:Nuclear Proteins / Q000276:immunology* / Q000302:isolation & purification; D012216:Rheumatic Diseases / Q000276:immunology*; D013194:Staining and Labeling
chemical_list: D000974:Antibodies, Antinuclear; D034961:Antigens, Nuclear; D001324:Autoantigens; D009687:Nuclear Proteins