cDNA cloning and expression of the Xenopus laevis vitellogenin receptor.
A Xenopus laevis oocyte cDNA library was screened with a PCR-generated X. laevis vitellogenin (VTG) receptor probe and a 3.6 kb cDNA clone containing the entire open reading frame, and 5' and 3' noncoding regions were isolated. The deduced amino acid sequence was 72% homologous to the chicken VLDL/VTG receptor, and the characteristic domains were highly conserved. Ligand binding analysis confirmed that the cloned receptor was Xenopus VTG-specific. Although Northern blotting analysis revealed that this gene was expressed as a major transcript of 3.6 kb in Xenopus ovary, weak but significant expression was observed in other tissues by RT-PCR analysis. The fact that major expression of the gene occurs in the ovary suggests that it has an important function in this organ.
Okabayashi
K
K
Department of Life Science, University of Tokyo, Japan.
Shoji
H
H
Nakamura
T
T
Hashimoto
O
O
Asashima
M
M
Sugino
H
H
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002645:Chickens; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D016384:Consensus Sequence; D017931:DNA Primers; D018076:DNA, Complementary; D004527:Egg Proteins; D005260:Female; D015870:Gene Expression; D015723:Gene Library; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D009865:Oocytes / Q000378:metabolism*; D009928:Organ Specificity; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D011956:Receptors, Cell Surface / Q000096:biosynthesis* / Q000737:chemistry / Q000378:metabolism; D011973:Receptors, LDL / Q000737:chemistry; D011994:Recombinant Proteins / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D014162:Transfection; D014819:Vitellogenins / Q000378:metabolism; D014982:Xenopus laevis
D017931:DNA Primers; D018076:DNA, Complementary; D004527:Egg Proteins; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D011973:Receptors, LDL; D011994:Recombinant Proteins; C036053:VLDL receptor; D014819:Vitellogenins; C030474:vitellogenin receptor