title: Cross-reactivity of antibodies raised to Pseudomonas fluorescens protease with extracellular proteins produced by meat-spoiling pseudomonads.
abstract: The cross-reactivity patterns of antibodies to Pseudomonas fluorescens protease with the extracellular proteins produced by a number of meat-spoiling pseudomonads were studied. Immunoblotting studies showed that purified IgG to Ps. fluorescens protease cross-reacted with extracellular proteins in the cell culture supernatant fluids of Pseudomonas spp., including Ps. fragi and Ps. lundensis. In the case of Ps. lundensis and Pseudomonas spp. 11390, the cross-reactive moieties were of similar molecular weight to the Ps. fluorescens protease (46 kDa). However, in Ps. fragi the cross-reactive moiety was a lower molecular weight protein (8 kDa). This may represent a fragment of the active enzyme. These results indicate the presence of common antigenic determinants among the proteases of meat spoiling pseudomonads.
authors.affiliation: Food Studies Research Unit, University of Ulster at Jordanstown, Newtownabbey, Co. Antrim, Northern Ireland, UK.
authors.forename: F T
authors.initials: FT
authors.lastname: Lundy
authors.forename: A C
authors.initials: AC
authors.lastname: Magee
authors.forename: I S
authors.initials: IS
authors.lastname: Blair
authors.forename: D A
authors.initials: DA
authors.lastname: McDowell
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology; D002417:Cattle; D003429:Cross Reactions; D010450:Endopeptidases / Q000276:immunology* / Q000302:isolation & purification; D015151:Immunoblotting; D008460:Meat / Q000382:microbiology*; D011551:Pseudomonas fluorescens / Q000201:enzymology* / Q000276:immunology; D011817:Rabbits; D013552:Swine
chemical_list: D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D010450:Endopeptidases