title: Role of granulocyte elastase in the formation of hemorrhagic shock-induced gastric mucosal lesions in the rat.
abstract: OBJECTIVE
  To investigate the mechanism by which activated leukocytes induce gastric mucosal lesions, we examined whether granulocyte elastase is involved in the formation of such lesions in a rat model of hemorrhagic shock.
  
  
  METHODS
  Prospective, randomized, controlled study.
  
  
  METHODS
  Research laboratory at a university medical center.
  
  
  METHODS
  Male Wistar rats, weighing 220 to 280 g.
  
  
  METHODS
  Animals were subjected to hemorrhagic shock by phlebotomy. ONO-5046, a granulocyte elastase inhibitor (300 mg/kg ip), was administered 30 mins before or after phlebotomy. The effects of antithrombotic substances and tranexamic acid on hemorrhagic shock-induced gastric mucosal lesions also were examined. The effects of granulocyte elastase on the thrombomodulin activity and 35S-glycosaminoglycan content of endothelial cells were examined, using cultured human umbilical vein endothelial cells.
authors.affiliation: Department of Laboratory Medicine, Kumamoto University Medical School, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Kushimoto
authors.forename: K
authors.initials: K
authors.lastname: Okajima
authors.forename: H
authors.initials: H
authors.lastname: Okabe
authors.forename: B R
authors.initials: BR
authors.lastname: Binder
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000201:enzymology; D005753:Gastric Mucosa / Q000187:drug effects / Q000503:physiopathology*; D005998:Glycine / Q000031:analogs & derivatives / Q000494:pharmacology / Q000627:therapeutic use; D006801:Humans; D019272:Leukocyte Elastase; D008297:Male; D010196:Pancreatic Elastase / Q000037:antagonists & inhibitors / Q000502:physiology*; D018962:Phlebotomy; D011446:Prospective Studies; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D015842:Serine Proteinase Inhibitors / Q000494:pharmacology / Q000627:therapeutic use; D012771:Shock, Hemorrhagic / Q000150:complications*; D013276:Stomach Ulcer / Q000209:etiology* / Q000517:prevention & control; D013449:Sulfonamides / Q000494:pharmacology / Q000627:therapeutic use
chemical_list: D015842:Serine Proteinase Inhibitors; D013449:Sulfonamides; C069195:sivelestat; D010196:Pancreatic Elastase; D019272:Leukocyte Elastase; D005998:Glycine