Simplified riboprobe purification using translucent straws as gel tubes.
Gel purification of radioactive riboprobes enhances the quality of the ribonuclease protection assay. A simple and effective method for riboprobe purification is described. The method uses acrylamide gels in plastic tubes to achieve electrophoretic separation of the RNA polymerase products.
Kol
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Department of Obstetrics and Gynecology, University of Maryland School of Medicine, Baltimore 21201, USA.
Ben-Shlomo
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Adashi
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Rohan
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RM
D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D015347:RNA Probes / Q000302:isolation & purification*; D051381:Rats
D015347:RNA Probes