title: Direct infection of CD34+ progenitor cells by human cytomegalovirus: evidence for inhibition of hematopoiesis and viral replication.
abstract: We successfully infected fluorescence-activated cell-sorted CD34+ cells from normal cord blood by the human cytomegalovirus (HCMV) laboratory strain Towne. An inhibitory effect of HCMV on clonogenic myeloid progenitors was observed in primary methylcellulose cultures. After an initial 7-day liquid culture of CD34(+)-infected cells, this inhibition was further amplified in secondary methylcellulose cultures, then involving both the myeloid and erythroid lineages. Under these conditions, viral DNA was detected both in erythroid and myeloid colonies using the polymerase chain reaction (PCR), but reverse transcription PCR (RT-PCR) failed to detect viral RNA. In contrast, when CD34(+)-infected cells were maintained in liquid suspension, both immediate, early, and late transcripts were detected as soon as day 3. In addition, viral production was demonstrated in the culture supernatants, thus confirming that a complete viral cycle occurred under liquid conditions. Furthermore, by resorting cells into CD34+ and CD34- fractions, we showed by RT-PCR that viral replication took place in cells still expressing CD34 antigen, whereas no RNA was found in more differentiated cells that had subsequently lost their CD34 antigen. These findings suggest that HCMV replication can occur at the early steps of progenitor differentiation and may be involved in the viral-induced myelosuppression.
authors.affiliation: Laboratory for Biology and Therapy of Immune Pathology, Hospital Pitié Salpêtrière, Paris, France.
authors.forename: M
authors.initials: M
authors.lastname: Movassagh
authors.forename: J
authors.initials: J
authors.lastname: Gozlan
authors.forename: B
authors.initials: B
authors.lastname: Senechal
authors.forename: C
authors.initials: C
authors.lastname: Baillou
authors.forename: J C
authors.initials: JC
authors.lastname: Petit
authors.forename: F M
authors.initials: FM
authors.lastname: Lemoine
mesh_terms: D018952:Antigens, CD34 / Q000032:analysis; D000956:Antigens, Viral / Q000032:analysis; D001483:Base Sequence; D002478:Cells, Cultured; D003587:Cytomegalovirus / Q000472:pathogenicity*; D017931:DNA Primers / Q000737:chemistry; D004279:DNA, Viral / Q000032:analysis; D005312:Fetal Blood; D015967:Gene Expression Regulation, Viral; D006410:Hematopoiesis; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000276:immunology / Q000382:microbiology*; D006801:Humans; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics; D012367:RNA, Viral / Q000032:analysis; D014779:Virus Replication
chemical_list: D018952:Antigens, CD34; D000956:Antigens, Viral; D017931:DNA Primers; D004279:DNA, Viral; D012333:RNA, Messenger; D012367:RNA, Viral