Distinct distribution of protein disulfide isomerase family proteins in rat tissues.
We investigated the distribution of protein disulfide isomerase (PDI) family proteins PDI, ERp72, and ERp61 in rat tissues and compared their localization by immunohistochemical double staining. The extent of their expression was diverse in different cell types, although they were ubiquitously distributed in a wide variety of cell types. Prominent staining for all three proteins was detected in thyroid follicular epithelia, tracheal mucous gland cells and chondrocytes, and chief cells and mucous neck cells of the stomach. Hepatocytes, neuronal cells in brain and spinal cord, and pancreatic acinar cells were also consistently and uniformly stained, but with low intensity. On the other hand, distinct differences were observed for the expression of the three proteins in plasma cells, pancreatic islet cells, goblet cells, and Paneth's cells of intestines, seminiferous epithelia, and salivary gland ductal epithelia. The similarities and differences in the distribution of the three proteins provide helpful clues to their biological functions.
Iida
K I
KI
The Second Department of Pathology, Nagoya University School of Medicine, Japan.
Miyaishi
O
O
Iwata
Y
Y
Kozaki
K I
KI
Matsuyama
M
M
Saga
S
S
D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D005260:Female; D005455:Fluorescent Antibody Technique; D006360:Heat-Shock Proteins / Q000378:metabolism*; D006801:Humans; D007535:Isomerases / Q000378:metabolism*; D008297:Male; D008562:Membrane Glycoproteins / Q000302:isolation & purification / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D019704:Protein Disulfide-Isomerases; D011817:Rabbits; D051381:Rats; D017208:Rats, Wistar; D014018:Tissue Distribution
D006360:Heat-Shock Proteins; D008562:Membrane Glycoproteins; C062477:endoplasmic reticulum glycoprotein p72; D007535:Isomerases; C513196:PDIA3 protein, rat; C513195:Pdia3 protein, mouse; D019704:Protein Disulfide-Isomerases; C085868:PDIA3 protein, human