Physiological measures of conduction velocity and refractory period for putative reward-relevant MFB axons arising in the rostral MFB.
Extracellular recordings were obtained, in urethane-anesthetized rats, from 44 neurons in the rostral bed nuclei of the medial forebrain bundle (MFB). These cells were antidromically activated by stimulation of MFB sites that typically support self-stimulation. Recording sites included the magnocellular preoptic nucleus, substantia innominata, ventral pallidum, olfactory tubercle, and horizontal limb of the diagonal band. Refractory period estimates ranged from 0.35 to 1.20 ms (mean +/- SD = 0.72 +/- 0.30 ms, n = 15) for stimulation sites in the lateral hypothalamic and ventral tegmental areas when using currents of twice threshold and procedures designed to estimate excitability at or near the site of stimulation. Interelectrode conduction velocity estimates ranged from 1.48 to 20.0 m/s (mean +/- SD = 9.26 +/- 7.22 m/s, n = 11) and were obtained by dividing the interelectrode distance by the difference in the response latency from the two MFB stimulation sites. The refractory period and conduction velocity estimates for these neurons overlap the psychophysically derived estimates for MFB reward neurons. These data are consistent with the hypothesis that neurons arising in the rostral bed nucleus of the MFB compose at least part of the directly activated substrate for MFB self-stimulation.
Murray
B
B
Department of Psychology, Concordia University, Montréal, Qué., Canada.
Shizgal
P
P
D000818:Animals; D001369:Axons / Q000502:physiology*; D004558:Electric Stimulation; D004567:Electrodes, Implanted; D004594:Electrophysiology; D007026:Hypothalamic Area, Lateral / Q000033:anatomy & histology / Q000166:cytology / Q000502:physiology; D008297:Male; D008474:Medial Forebrain Bundle / Q000033:anatomy & histology / Q000166:cytology / Q000502:physiology*; D008839:Microelectrodes; D009431:Neural Conduction / Q000502:physiology*; D051381:Rats; D012032:Refractory Period, Electrophysiological / Q000502:physiology*; D012201:Reward; D017557:Ventral Tegmental Area / Q000033:anatomy & histology / Q000166:cytology / Q000502:physiology