Proton nuclear magnetic resonance spectroscopy of lactate production in isolated rat liver during cold preservation.
Lactate-edited 1H NMR difference spectra have been acquired from intact rat liver tissue following flushing and preservation in ice. A peak, initially at 1.26 ppm, was seen to increase in the liver tissue with preservation time. This peak was assigned to lactate, despite the fact that its chemical shift was initially shifted by approximately -0.1 ppm relative to an externally added standard. The assignment was based on the following: (a) the peak increased over a 24-h ischemic storage period; (b) it was coupled to a signal 2.78 +/- 0.02 ppm upfield; and (c) a parallel increase in lactate was noted in perchloric acid extracts of tissue from the same liver. An additional peak, assigned to alanine, was also observed during storage and was also shifted by approximately -0.1 ppm. Inclusion of dimethyl sulfoxide, which readily permeates liver tissue, demonstrated that this chemical shift alteration was a tissue-specific effect. These results demonstrate that 1H NMR spectroscopy of intact liver tissue during hypothermic ischemia is possible, though chemical shift assignments should be made with caution.
Lockett
C J
CJ
Institute of Child Health, Royal College of Surgeons Unit of Biophysics, London, United Kingdom.
Busza
A L
AL
Proctor
E
E
Churchill
T A
TA
Williams
S R
SR
Fuller
B J
BJ
D000818:Animals; D003080:Cold Temperature; D066298:In Vitro Techniques; D007773:Lactates / Q000096:biosynthesis*; D019344:Lactic Acid; D008099:Liver / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D009926:Organ Preservation / Q000379:methods*; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
D007773:Lactates; D019344:Lactic Acid