Differentiation of Borrelia burgdorferi sensu lato strains isolated from skin biopsies and tick by pulsed-field gel electrophoresis.
Fifteen isolates of Borrelia burgdorferi sensu lato from skin biopsies (8) and ticks (7) were investigated for species differentiation by pulsed-field gel electrophoresis (PFGE). The skin isolates were further characterized by plasmid profile as well as SDS-PAGE and revealed six Borrelia afzelii-, one Borrelia burgdorferi sensu stricto-, and one Borrelia garinii species. The tick isolates showed three Borrelia afzelii--and four Borrelia garinii species. The skin isolates could be further discriminated by plasmid profile analysis--all strains showed an individual pattern. The SDS-PAGE of whole-cell lysates obtained from skin isolate showed a different protein pattern for each of the skin isolates.
Busch
U
U
Max von Pettenkofer-Institut für Hygiene and Medizinische Mikrobiologie, Ludwig Maximilians Universität München, Germany.
Hizo Teufel
C
C
Boehmer
R
R
Preacmursic
V
V
D000818:Animals; D015748:Borrelia burgdorferi Group / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000032:analysis; D016521:Electrophoresis, Gel, Pulsed-Field; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D066298:In Vitro Techniques; D012150:Polymorphism, Restriction Fragment Length; D012867:Skin / Q000382:microbiology*; D013045:Species Specificity; D013987:Ticks / Q000382:microbiology
D004269:DNA, Bacterial