title: pMPY-ZAP: a reusable polymerase chain reaction-directed gene disruption cassette for Saccharomyces cerevisiae.
abstract: Gene disruption is an important method for genetic analysis in Saccharomyces cerevisiae. We have designed a polymerase chain reaction-directed gene disruption cassette that allows rapid disruption of genes in S. cerevisiae without previously cloning them. In addition, this cassette allows recycling of URA3, generating gene disruptions without the permanent loss of the ura3 marker. An indefinite number of disruptions can therefore be made in the same strain.
authors.affiliation: Cold Spring Harbor Laboratory, NY 11724, USA.
authors.forename: B L
authors.initials: BL
authors.lastname: Schneider
authors.forename: B
authors.initials: B
authors.lastname: Steiner
authors.forename: W
authors.initials: W
authors.lastname: Seufert
authors.forename: A B
authors.initials: AB
authors.lastname: Futcher
mesh_terms: D001483:Base Sequence; D005819:Genetic Markers; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional / Q000379:methods*; D009964:Orotidine-5'-Phosphate Decarboxylase / Q000235:genetics; D016133:Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D012641:Selection, Genetic; D014170:Transformation, Genetic
chemical_list: D005819:Genetic Markers; D009964:Orotidine-5'-Phosphate Decarboxylase