title: [Fungi and yeasts isolated in mycological studies in skin and nail infections in The Netherlands, 1992-1993].
abstract: OBJECTIVE
  To describe fungi and yeasts isolated from skin and nail infections in the Netherlands.
  
  
  METHODS
  Retrospective.
  
  
  METHODS
  Centraalbureau voor Schimmelcultures (CBS), Baarn, the Netherlands.
  
  
  METHODS
  Results of mycological investigation of skin and nail samples in the period 1992-1993 were analysed. After a clinical diagnosis of mycosis, performed by dermatologists and general practitioners, material was sent to the CBS for mycological research.
  
  
  RESULTS
  The clinical diagnosis of onychomycosis was rather accurate, especially if made performed by dermatologists. Mycoses of the skin were sometimes confused with other skin diseases. When microscopical observation showed a positive result, 93% of the cultures were positive as well. The main agent of onychomycosis was Trichophyton rubrum; T. mentagrophytes was more frequently isolated from tinea manuum/pedis and T. tonsurans from tinea corporis/cruris. Epidermophyton floccosum was only isolated from skin lesions and Microsporum canis, T. soudanense and T. verrucosum only from tinea corporis/cruris. The most important yeasts isolated were Trichosporon mucoides, Candida guilliermondii, C. parapsilosis, C. famata and Malassezia furfur. Other fungi isolated were either pigmented (melanin, carotene), thermophilic or belonged to the order of the Onygenales.
  
  
  CONCLUSIONS
  Mycological research to confirm the clinical diagnosis of a skin mycosis is advisable. Species isolated differed in their predilection for different parts of the human body. Yeasts were mainly isolated as double infections. Apart from the dermatophytes there is a special group of fungi which can cause mycoses.
authors.affiliation: Centraalbureau voor Schimmelcultures, afd. Toegepast onderzoek, Baarn.
authors.forename: A F
authors.initials: AF
authors.lastname: Kuijpers
authors.forename: C S
authors.initials: CS
authors.lastname: Tan
mesh_terms: D003881:Dermatomycoses / Q000382:microbiology*; D005658:Fungi / Q000302:isolation & purification*; D006801:Humans; D014009:Onychomycosis / Q000382:microbiology*; D012189:Retrospective Studies; D015003:Yeasts / Q000302:isolation & purification*
vernacular_title: Schimmels en gisten gevonden bij mycologisch onderzoek van huid- en nagelinfecties in Nederland, 1992-1993.