In vivo microdialysis determination of collagen-induced serotonin release in rat blood.
We developed a sensitive microbore HPLC method coupled with an on-line microdialysis system to simultaneously measure endogenous 5-hydroxytryptamine (serotonin; 5-HT) and its major metabolite 5-hydroxyindoleacetic acid (5-HIAA) in the rat blood in vivo. A dialysis tube was placed in the right jugular vein. The validity of the procedure is demonstrated because analysis of the aggregating agents, collagen (1 mg/kg) plus epinephrine (0.3 mg/kg) after intravenous injection, showed that they induced an increase in 5-HT and 5-HIAA levels in the jugular vein of the rat.
Tsai
T H
TH
Department of Pharmacology, National Research Institute of Chinese Medicine, Taipei, Taiwan.
Chen
C F
CF
D000818:Animals; D003094:Collagen / Q000494:pharmacology*; D004837:Epinephrine / Q000494:pharmacology; D006897:Hydroxyindoleacetic Acid / Q000097:blood; D007700:Kinetics; D008297:Male; D017551:Microdialysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D012701:Serotonin / Q000097:blood*
D012701:Serotonin; D006897:Hydroxyindoleacetic Acid; D003094:Collagen; D004837:Epinephrine