[Expression of oncogene and anti-oncogene in mouse lung cancer induced by coal-burning smoke].
Previous epidemiology studies have shown association between coal burning and human lung cancer. To confirm relationship of coal burning to lung cancer formation and progression the expression of p53 and c-myc in 13 mouse lung cancer induced by coal-burning smoke and 5 mouse lung tissue control was studied by DNA-RNA in situ hybridization (ISH). Nine of 13 specimens showed c-myc overexpression but it occurred only 1 in 9 in the adjacent tissue. There was overexpression of p53 mRNA in all 13 lung cancer and 5 adjacent tissues. None in the controls was expression of p53 and c-myc detected. When compared to controls, there was significant higher expression of c-myc gene (P = 0.002) and p53 gene (P = 0.0001). The results confirm that overexpression of p53 and c-myc are common molecular events of lung cancer by coal-burning smoke and provide further evidence that smoke from coal burning is a causative agent of lung cancer.
Lin
C
C
Cancer Research Institute, Harbin Medical University.
Dai
X
X
Sun
X
X
D000230:Adenocarcinoma / Q000209:etiology / Q000235:genetics*; D000818:Animals; D003031:Coal / Q000009:adverse effects*; D005260:Female; D015870:Gene Expression; D016259:Genes, myc; D016158:Genes, p53; D008175:Lung Neoplasms / Q000209:etiology / Q000235:genetics*; D008297:Male; D051379:Mice; D012333:RNA, Messenger / Q000096:biosynthesis; D012906:Smoke / Q000009:adverse effects*
D003031:Coal; D012333:RNA, Messenger; D012906:Smoke