title: [Tissue lipid peroxidation in nutritional encephalomalacia of broiler chickens].
abstract: The consequences of different dietary fats in combination with two vitamin E levels on peroxidative tissue damage of chicken brain and liver and its meaning for development of nutritional encephalomalacia (NE) were investigated. A feeding experiment was performed with 1-day-old chickens from hens on a vitamin-E-poor diet. The animals received a vitamin-E-deficient basic diet containing 10% fat, rich in either C18:3n3-, C18:2n6- or C18:1n9-fatty acids. The fat was given either fresh or oxidized (peroxidation number: 250) and 0 or 50 ppm alpha-tocopherylacetate was added. Typical symptoms of NE occurred mainly in those groups fed with n6-fatty acids beginning on day 7. In order to evaluate oxidative tissue damage, conjugated dienes, fluorescent pigments and TBA-reactive substances were determined in liver, cerebrum and cerebellum. Brain was examined histologically. In liver and cerebrum, the feeding of oxidized fats led to a 20% increase in conjugated dienes. Fluorescent pigments could be determined only in the brain tissues. However, feeding conditions had no effect, although autofluorescence was observed histologically in the affected animals. TBA-reactive substances were heightened in cerebrum (30%) and liver (130%) as a result of feeding linolenic acid. Vitamin E deficiency doubled TBA-reactive substances only in the liver. The parameters measured did not show intensified lipid peroxidation in the cerebellum of the animals fed the NE producing diet. Rather, the liver seems to be affected by the oxidative stress.
authors.affiliation: Institut für Physiologische Chemie, Hannover, Deutschland.
authors.forename: H
authors.initials: H
authors.lastname: Fuhrmann
authors.forename: S
authors.initials: S
authors.lastname: Schultheis
authors.forename: W
authors.initials: W
authors.lastname: Drommer
authors.forename: F J
authors.initials: FJ
authors.lastname: Kaup
authors.forename: H P
authors.initials: HP
authors.lastname: Sallmann
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism / Q000473:pathology; D002645:Chickens; D004041:Dietary Fats / Q000008:administration & dosage / Q000494:pharmacology; D004678:Encephalomalacia / Q000209:etiology / Q000378:metabolism / Q000662:veterinary*; D005260:Female; D015227:Lipid Peroxidation / Q000502:physiology*; D008099:Liver / Q000378:metabolism / Q000473:pathology; D011201:Poultry Diseases / Q000209:etiology / Q000378:metabolism*; D014810:Vitamin E / Q000008:administration & dosage / Q000494:pharmacology; D014811:Vitamin E Deficiency / Q000150:complications / Q000662:veterinary*
chemical_list: D004041:Dietary Fats; D014810:Vitamin E
vernacular_title: Zur Erfassung der Gewebs-Lipidperoxidation bei der Nutritiven Enzephalomalazie des Broilers.