Modulation of idiotypic and antiidiotypic immunoglobulin G responses in an alloimmune thrombocytopenic patient associated with extracorporeal protein A immunoadsorption.
In the present case study, a patient with Non-Hodgkin. Lymphoma underwent combination chemotherapy resulting in severe pancytopenia requiring transfusion support with blood products. The patient became refractory to random donor platelet transfusions and subsequently received five immunoadsorption treatments. The patient's clinical response to immunoadsorption therapy was assessed by monitoring platelet transfusion recovery and survival. In addition, changes in antibody responses were assessed. Early during the course of immunoadsorption therapy, antiplatelet immunoglobulin G (IgG) alloantibody was detected. There was a decline in antiplatelet IgG alloantibody levels by the last immunoadsorption treatment associated with increases to platelet correct count increments after completion of immunoadsorption therapy. In addition, elevated levels of antiidiotypic IgG antibody detected early during the course of therapy were significantly reduced by the last immunoadsorption treatment. This case study suggests that specific alloimmune idiotypic IgG antibody and corresponding antiidiotypic IgG antibody responses may be modulated in association with extracorporeal immunoadsorption employing protein A/silica columns.
Balint
J P
JP
IMRE Corporation, Seattle, Washington, U.S.A.
Hussein
M A
MA
Quagliata
F
F
Cochran
S
S
Jones
F R
FR
D000293:Adolescent; D000888:Antibodies, Anti-Idiotypic / Q000096:biosynthesis / Q000276:immunology; D001792:Blood Platelets / Q000276:immunology*; D004359:Drug Therapy, Combination; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis* / Q000276:immunology; D007130:Immunoglobulin Idiotypes / Q000096:biosynthesis / Q000276:immunology; D007163:Immunosorbent Techniques; D007518:Isoantibodies / Q000276:immunology; D008228:Lymphoma, Non-Hodgkin / Q000188:drug therapy; D008297:Male; D010198:Pancytopenia / Q000139:chemically induced / Q000276:immunology / Q000628:therapy; D017713:Platelet Transfusion; D013205:Staphylococcal Protein A / Q000378:metabolism*; D013921:Thrombocytopenia / Q000139:chemically induced / Q000276:immunology* / Q000628:therapy
D000888:Antibodies, Anti-Idiotypic; D007074:Immunoglobulin G; D007130:Immunoglobulin Idiotypes; D007518:Isoantibodies; D013205:Staphylococcal Protein A