[Analysis of penbutolol and its metabolites in human body fluid by gas chromatography--mass spectrometry].
A method for the detection and identification of penbutolol and its metabolites in human body fluid by using gas chromatography-mass spectrometry was established. The urine was extracted with diethyl ether-isopropanol and the plasma was extracted with Sep-Pak column. After TMS derivatization the sample was analyzed with GC-MSD. Six metabolites of penbutolol were found in urine sample. The recovery was 90.83% for urine and 85.88% for plasma. The detection limit of penbutolol was 5 pg.
Shen
L
L
China Doping Control Center, National Research Institute of Sports Medicine, Beijing.
Xu
Y X
YX
Zhang
C J
CJ
Wu
Y
Y
Wang
S
S
Cui
K R
KR
D000319:Adrenergic beta-Antagonists / Q000378:metabolism* / Q000652:urine; D000328:Adult; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D008297:Male; D010394:Penbutolol / Q000378:metabolism* / Q000652:urine
D000319:Adrenergic beta-Antagonists; D010394:Penbutolol