Human cytomegalovirus is not implicated in benign prostatic hyperplasia: a study using immunohistochemistry and the polymerase chain reaction.
OBJECTIVE
To evaluate whether human cytomegalovirus (CMV) may play a role in the pathogenesis of benign prostatic hyperplasia.


METHODS
We used immunohistochemistry and the polymerase chain reaction (PCR) to evaluate the presence of CMV in normal prostatic tissue (n = 12) and BPH tissue (n = 21).


RESULTS
Immunostaining for CMV in prostatic tissue was negative; however, staining was evident in mononuclear cells seen in the tissues of both groups. With nested PCR, CMV genomic material was demonstrated in 1 tissue specimen from each group (p > 0.1).


CONCLUSIONS
These data suggest that CMV is unlikely to be a significant factor in the pathogenesis of BPH.
Stapleton
A M
AM
Matsunaga-Conte Prostate Cancer Research Center, Scott Department of Urology, Baylor College of Medicine, Houston, Texas 77030, USA.
Williams
R H
RH
Timme
T L
TL
Yang
G
G
Truong
L D
LD
Thompson
T C
TC
D001483:Base Sequence; D003587:Cytomegalovirus / Q000235:genetics / Q000302:isolation & purification*; D004279:DNA, Viral / Q000032:analysis; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011470:Prostatic Hyperplasia / Q000821:virology*
D004279:DNA, Viral