Antioxidant survey to assess antagonism to redox stress using a prokaryotic and an eukaryotic system.
Using a prokaryote (Escherichia coli) and a metazoa-resembling eukaryote (Ochromonas danica), we surveyed antioxidants which might overcome redox stress imposed by menadione sodium bisulphite (MD) and buthionine sulphoximine (BSO). BSO oxidant stress was evident only in O. danica; MD oxidant stress was evident in both organisms. Glutathione, its precursors, e.g. cysteine, homocysteine, and 2-oxo-4-thiazolidine carboxylic acid, and red blood cells, emerged as prime antioxidants for relieving BSO and MD oxidant stress. BSO and MD oxidant activity and antioxidant-annulling effect in O. danica were judged comparable to those found in animal cells whereas the results E. coli were not entirely equivalent. The O. danica system emerged as a practical, rapid, and useful system for pinpointing oxidant stressors and antioxidants, and shows promise for studies with mammalian systems.
Baker
H
H
Department of Preventive Medicine, New Jersey Medical School, Newark 07107, USA.
DeAngelis
B
B
Frank
O
O
Khalil
M
M
Hutner
S H
SH
Baker
E R
ER
D000975:Antioxidants / Q000494:pharmacology*; D019328:Buthionine Sulfoximine; D003545:Cysteine / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects* / Q000254:growth & development; D056890:Eukaryota / Q000187:drug effects* / Q000254:growth & development; D005609:Free Radicals; D005978:Glutathione / Q000494:pharmacology; D006710:Homocysteine / Q000494:pharmacology; D008717:Methionine Sulfoximine / Q000031:analogs & derivatives / Q000494:pharmacology; D018384:Oxidative Stress / Q000187:drug effects*; D011761:Pyrrolidonecarboxylic Acid; D013844:Thiazoles / Q000494:pharmacology; D053778:Thiazolidines; D014812:Vitamin K / Q000031:analogs & derivatives / Q000494:pharmacology; D024483:Vitamin K 3
D000975:Antioxidants; D005609:Free Radicals; D013844:Thiazoles; D053778:Thiazolidines; D006710:Homocysteine; D014812:Vitamin K; D008717:Methionine Sulfoximine; D019328:Buthionine Sulfoximine; D024483:Vitamin K 3; D005978:Glutathione; D003545:Cysteine; D011761:Pyrrolidonecarboxylic Acid; C029536:2-oxothiazolidine-4-carboxylic acid