Mini-chromosomes derived from the human Y chromosome by telomere directed chromosome breakage.
We have used telomeric DNA to break two acrocentric derivatives of the human Y chromosome into mini-chromosomes that are small enough to be size- fractionated by pulsed-field gel electrophoresis. One of the mini-chromosomes is about 7 Mb in size and sequence-tagged site analysis of this molecule suggests that it corresponds to a simple truncation of the short arm of the Y chromosome. Five of the mini-chromosomes are derived from the long arm, are all rearranged by more than a simple truncation, and range in size from 4.0 Mb to 9 Mb. We have studied the mitotic stabilities of these mini-chromosomes and shown that they are stably maintained by cells proliferating in culture for about 100 cell divisions.
Heller
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R
Cancer Research Campaign Chromosome Molecular Biology Group, Biochemistry Department, Oxford University, England.
Brown
K E
KE
Burgtorf
C
C
Brown
W R
WR
D000818:Animals; D016466:CHO Cells; D002262:Carboxy-Lyases / Q000096:biosynthesis / Q000235:genetics; D002455:Cell Division; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D002874:Chromosome Mapping; D006224:Cricetinae; D005800:Genes, Fungal; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008938:Mitosis; D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000235:genetics; D016324:Sequence Tagged Sites; D016615:Telomere; D014162:Transfection; D014998:Y Chromosome
D002262:Carboxy-Lyases; C022378:phosphoribosylaminoimidazole carboxylase