title: Rederivation of mice by means of in vitro fertilization and embryo transfer.
abstract: In vitro fertilization and embryo transfer were performed for rederivation of four strains of mice harbouring mouse hepatitis virus (MHV) and/or Pasteurella pneumotropica (P. pneumotropica). Superovulated oocytes were fertilized by preincubated cauda epididymis sperm in vitro. Fertilized eggs at 2-cell stage were transferred into the oviducts of specific pathogen free (SPF) recipients. Microbial examination of sperm and/or oocyte donors verified the presence of P. pneumotropica and/or of antibodies to MHV in all strains, but neither in the recipients nor in the offspring antibodies to MHV could they be detected. The results indicate that an in vitro fertilization-embryo transfer (IVF-ET) system is an effective and simple alternative to cesarean operation in infected mice.
authors.affiliation: Chugai Pharmaceutical Co., Ltd, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Suzuki
authors.forename: K
authors.initials: K
authors.lastname: Yorozu
authors.forename: T
authors.initials: T
authors.lastname: Watanabe
authors.forename: M
authors.initials: M
authors.lastname: Nakura
authors.forename: J
authors.initials: J
authors.lastname: Adachi
mesh_terms: D000818:Animals; D000831:Animals, Newborn / Q000382:microbiology; D001947:Breeding / Q000379:methods*; D004624:Embryo Transfer / Q000379:methods*; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008822:Mice, Transgenic; D006517:Murine hepatitis virus / Q000302:isolation & purification*; D009865:Oocytes / Q000382:microbiology; D010325:Pasteurella / Q000302:isolation & purification*; D012107:Research Design; D013047:Specific Pathogen-Free Organisms; D013094:Spermatozoa / Q000382:microbiology