The specificity and product of quenching singlet oxygen by 2,2,6,6-tetramethylpiperidine.
The specificity of 2,2,6,6-tetramethylpiperidine to singlet oxygen was shown using Rose Bengal as a singlet oxygen generator, and Xanthine-Xanthine Oxidase and KO2 as the sources for the superoxide radical. The highest concentration of produced-singlet oxygen occurred at 25% of O2 by Rose Bengal photosensitization. The linewidth of the EPR signal for photosensitized nitroxyl radical, increasing solvent polarity. Deuterated solvents enlarge the EPR signal intensity in a dose-dependent manner. No EPR signal increase was observed in xanthine-xanthine oxidase reaction or KO2 systems, indicating that TEMP does not react with the superoxide anion. Thus, reaction of TEMP with 1O2 is highly specific.
Zang
L Y
LY
Department of Biomedical Sciences, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Tech, Blacksburg 24061-0442, USA.
van Kuijk
F J
FJ
Misra
B R
BR
Misra
H P
HP
D003497:Cyclic N-Oxides / Q000737:chemistry; D004578:Electron Spin Resonance Spectroscopy; D005609:Free Radicals / Q000737:chemistry; D010100:Oxygen / Q000737:chemistry*; D010880:Piperidines / Q000737:chemistry*; D012395:Rose Bengal / Q000737:chemistry*; D014969:Xanthine Oxidase / Q000737:chemistry
D003497:Cyclic N-Oxides; D005609:Free Radicals; D010880:Piperidines; D012395:Rose Bengal; D014969:Xanthine Oxidase; D010100:Oxygen; C003959:TEMPO