Expression of Epstein-Barr virus latent infection genes and oncogenes in lymphoma cell lines derived from pyothorax-associated lymphoma.
Malignant lymphomas frequently develop in the pleural cavity of patients with long-standing pyothorax. The term pyothorax-associated lymphoma (PAL) has been proposed for this type of tumor. Most PALs are diffuse lymphomas of the B-cell type and contain Epstein-Barr virus (EBV) DNA. We have established 2 lymphoma cell lines from biopsy specimens of PAL cases, OPL-1 and OPL-2, and examined their growth characteristics and the expression of EBV latent infection genes and oncogenes. OPL-2 exhibited a more rapid growth and higher saturation density than OPL-1, and only OPL-2 exhibited colony-forming activity in soft agar. OPL-1 and -2 were positive for B-cell differentiation markers and showed clonal surface immunoglobulins. Both line contained a single predominant form of episomal EBV DNA, indicating clonal cellular proliferation of an EBV-infected progenitor cell. OPL-1 and -2 contained type B and A EBV genome, respectively. Expression of EBV nuclear antigen (EBNA)2 mRNA and protein was detected by Northern and Western blot analysis in OPL-1, but not in OPL-2. On the other hand, the expression of latent membrane protein (LMP)1 mRNA in both OPL-1 and -2 was extremely weak and detectable only by reverse transcription-polymerase chain reaction. Protein expression of LMP1 was not observed by Western blot analysis or immunocytochemistry. Both lines expressed c-myc mRNA. Only OPL-1 expressed mRNA of c-fgr, an oncogene whose expression is upregulated by EBNA2. Both OPLs expressed bcl-2 mRNA without detectable expression of LMP1 protein.
Kanno
H
H
Departmemt of Pathology, Osaka University Medical School, Japan.
Yasunaga
Y
Y
Ohsawa
M
M
Taniwaki
M
M
Iuchi
K
K
Naka
N
N
Torikai
K
K
Shimoyama
M
M
Aozasa
K
K
D000368:Aged; D000956:Antigens, Viral / Q000032:analysis; D001483:Base Sequence; D002869:Chromosome Aberrations; D004268:DNA-Binding Proteins / Q000032:analysis; D016724:Empyema, Pleural / Q000150:complications*; D019309:Epstein-Barr Virus Nuclear Antigens; D015321:Gene Rearrangement; D005803:Genes, Immunoglobulin; D016679:Genome, Viral; D004854:Herpesvirus 4, Human / Q000235:genetics*; D006801:Humans; D016130:Immunophenotyping; D008223:Lymphoma / Q000235:genetics / Q000821:virology*; D008297:Male; D008969:Molecular Sequence Data; D009857:Oncogenes; D014407:Tumor Cells, Cultured; D014763:Viral Matrix Proteins / Q000032:analysis
D000956:Antigens, Viral; D004268:DNA-Binding Proteins; C037662:EBV-associated membrane antigen, Epstein-Barr virus; D019309:Epstein-Barr Virus Nuclear Antigens; D014763:Viral Matrix Proteins