title: Synthesis of potato virus X RNAs by membrane-containing extracts.
abstract: Membrane-containing extracts isolated from tobacco plants infected with the plus-strand RNA virus, potato virus X (PVX), supported synthesis of four major, high-molecular-weight PVX RNA products (R1 to R4). Nuclease digestion and hybridization studies indicated that R1 and R2 are a mixture of partially single-stranded replicative intermediates and double-stranded replicative forms. R3 and R4 are double-stranded products containing sequences typical of the two major PVX subgenomic RNAs. The newly synthesized RNAs were demonstrated to have predominantly plus-strand polarity. Synthesis of these products was remarkably stable in the presence of ionic detergents.
authors.affiliation: Department of Biochemistry, North Carolina State University, Raleigh, North Carolina 27695-7622, USA.
authors.forename: S V
authors.initials: SV
authors.lastname: Doronin
authors.forename: C
authors.initials: C
authors.lastname: Hemenway
mesh_terms: D004720:Endonucleases / Q000378:metabolism; D006037:Glyoxal / Q000494:pharmacology; D008836:Micrococcal Nuclease / Q000378:metabolism; D010936:Plant Extracts; D010947:Plants, Toxic; D017863:Potexvirus / Q000235:genetics*; D012367:RNA, Viral / Q000096:biosynthesis*; D014026:Nicotiana / Q000378:metabolism
chemical_list: D010936:Plant Extracts; D012367:RNA, Viral; D006037:Glyoxal; D004720:Endonucleases; D008836:Micrococcal Nuclease