title: The influence of a pneumoperitoneum on the peritoneal implantation of free intraperitoneal colon cancer cells.
abstract: BACKGROUND
  In order to test the influence of a pneumoperitoneum on the peritoneal implantation of free intraperitoneal colon cancer cells, 40 male syngeneic WAG rats were at random divided into four groups.
  
  
  METHODS
  Group 1 (n = 10) animals underwent a midline laparotomy and 10(4) CC531 colon cancer cells were injected intraperitoneally (IP); in group 2 (n = 10) 10(4) CC531 cells were injected IP without further manipulation; in group 3 (n = 10) a pneumoperitoneum up to 10 mmHg was created after the IP injection of the same amount of CC531 cells. The pneumoperitoneum was maintained for 15 min. Finally in group 4 (n = 10) after the IP injection of 10(4) CC531 cells and after the creation of a pneumoperitoneum, two 14-G IV catheters simulating trocars were introduced in each flank. A follow-up period of 8 weeks was used. Tumor implantation was scored according to the peritoneal cancer index of Eggermont and the index of Chauffert.
  
  
  RESULTS
  Tumor nodules were found varying from 60% in groups 1-3 to 50% in group 4. There was no statistical difference between the implantation rate in the four groups. A port-site recurrence was seen in group 4; all the other tumor implants were located in the mesenterium, omentum, internal genitals, or parietal peritoneum.
  
  
  CONCLUSIONS
  The presence of a pneumoperitoneum does not enhance the implantation of free intraperitoneal malignant colon cancer cells in the rat, but the presence of a "port" may lead to abdominal-wall metastases.
authors.affiliation: Laboratory of Experimental Surgery, University of Antwerp, Wilrijkstraat 10, 2650 Antwerp, Belgium.
authors.forename: G
authors.initials: G
authors.lastname: Hubens
authors.forename: M
authors.initials: M
authors.lastname: Pauwels
authors.forename: A
authors.initials: A
authors.lastname: Hubens
authors.forename: P
authors.initials: P
authors.lastname: Vermeulen
authors.forename: E
authors.initials: E
authors.lastname: Van Marck
authors.forename: E
authors.initials: E
authors.lastname: Eyskens
mesh_terms: D000230:Adenocarcinoma / Q000556:secondary*; D000818:Animals; D003110:Colonic Neoplasms / Q000473:pathology*; D004195:Disease Models, Animal; D005500:Follow-Up Studies; D008297:Male; D009368:Neoplasm Transplantation / Q000379:methods; D010534:Peritoneal Neoplasms / Q000556:secondary*; D010537:Peritoneum / Q000601:surgery*; D011028:Pneumoperitoneum, Artificial; D051381:Rats; D014407:Tumor Cells, Cultured