Effects of calcitonin gene-related peptide and interleukin 6 on myoblast differentiation.
Effects of calcitonin gene-related peptide (CGRP) and interleukin 6 (IL-6) on muscle cell differentiation were studied using cultured rat myoblasts (L6 cells). Cell morphology and the amounts of the messenger RNAs (mRNAs) of myogenin and Myf-5, DNA content, creatine kinase (CK) activity, and myoglobin (Mb) content in the cultured cells were examined serially over 10 days of culture. In the presence of CGRP or IL-6, the mRNAs of myogenin and Myf-5 were expressed earlier and at a higher concentration in the treated cells than in the control cells. The ratios of CK activity to DNA content (CK/DNA) and of Mb content to DNA content (Mb/DNA) on day 10 of culture also were greater than in the control cells. Furthermore, the mRNAs of myogenin and Myf-5 in cultured cells incubated with both CGRP and IL-6 increased more rapidly than in cells cultured with CGRP or IL-6 alone, and the ratios of CK/DNA and Mb/DNA on day 10 were more than twice those in the presence of CGRP or IL-6. These findings indicate that both CGRP and IL-6 facilitate the differentiation of myoblasts and may have an additive effect.
Okazaki
S
S
First Department of Internal Medicine, School of Medicine, University of Tokushima, Japan.
Kawai
H
H
Arii
Y
Y
Yamaguchi
H
H
Saito
S
S
D000818:Animals; D001483:Base Sequence; D015740:Calcitonin Gene-Related Peptide / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D003402:Creatine Kinase / Q000378:metabolism; D004247:DNA / Q000096:biosynthesis; D017931:DNA Primers / Q000737:chemistry; D004268:DNA-Binding Proteins; D018507:Gene Expression Regulation, Developmental; D015850:Interleukin-6 / Q000494:pharmacology*; D008969:Molecular Sequence Data; D009124:Muscle Proteins / Q000235:genetics; D018482:Muscle, Skeletal / Q000166:cytology*; D051787:Myogenic Regulatory Factor 5; D018008:Myogenin / Q000235:genetics; D009211:Myoglobin / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D013997:Time Factors; D015534:Trans-Activators
D017931:DNA Primers; D004268:DNA-Binding Proteins; D015850:Interleukin-6; D009124:Muscle Proteins; C503803:Myog protein, rat; D051787:Myogenic Regulatory Factor 5; D018008:Myogenin; D009211:Myoglobin; D012333:RNA, Messenger; D015534:Trans-Activators; D004247:DNA; D003402:Creatine Kinase; D015740:Calcitonin Gene-Related Peptide