Recognition of CD52 allelic gene products by CAMPATH-1H antibodies.
Cloning of the CD52 from a B-lymphocyte tumour cDNA library revealed two closely related sequences differing only at two amino acids C-terminal to the proposed point of glycosylphosphatidylinositol (GPI)-linkage. When transfected into CHO cells only one of these sequences gave high-level expression of the antigen recognized by the prototypic anti-CD52 antibody CAMPATH-1 whereas in JURKAT cells good expression levels were obtained with both sequences. Fusion of the sequence from the second sequence to DNA encoding the extracellular domain of CD4 indicated that this sequence was capable of directing GPI linkage. The possible implications for the function of CD52 and serotherapy with anti-CD52 antibodies are discussed.
Hale
C
C
Molecular Immunology, Wellcome Research Laboratories, Kent, UK.
Bartholomew
M
M
Taylor
V
V
Stables
J
J
Topley
P
P
Tite
J
J
D000074323:Alemtuzumab; D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D061067:Antibodies, Monoclonal, Humanized; D000912:Antibodies, Neoplasm / Q000276:immunology*; D000937:Antigen-Antibody Reactions; D015703:Antigens, CD / Q000235:genetics* / Q000276:immunology; D000951:Antigens, Neoplasm; D001483:Base Sequence; D000074301:CD52 Antigen; D016466:CHO Cells; D002460:Cell Line; D003001:Cloning, Molecular; D006224:Cricetinae; D017931:DNA Primers / Q000235:genetics; D015870:Gene Expression; D005821:Genetic Techniques; D006023:Glycoproteins; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013601:T-Lymphocytes / Q000276:immunology
D000911:Antibodies, Monoclonal; D061067:Antibodies, Monoclonal, Humanized; D000912:Antibodies, Neoplasm; D015703:Antigens, CD; D000951:Antigens, Neoplasm; D000074301:CD52 Antigen; C000613862:CD52 protein, human; D017931:DNA Primers; D006023:Glycoproteins; D000074323:Alemtuzumab