Reversed-phase liquid chromatography on a microspherical carbon column at high temperature.
The chromatographic conditions for the use of a spherical carbon column at high temperatures up to 160 degrees C are described. The column heating system and the eluate cooling system are also described. Under the conditions studied, it was found that the carbon column at elevated temperature was stable and could be applicable to any compounds that are durable under these conditions. The peptide bonds at this high temperature and at this acidity were still stable enough for analysis, but the indole residues showed poor stability.
Yamaki
S
S
Department of Chemistry, Faculty of Science, Tokyo Metropolitan University, Japan.
Isobe
T
T
Okuyama
T
T
Shinoda
T
T
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis / Q000737:chemistry; D000803:Angiotensin I / Q000032:analysis / Q000737:chemistry; D002244:Carbon; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D008863:Microspheres; D008969:Molecular Sequence Data; D013373:Substance P / Q000032:analysis / Q000737:chemistry; D014364:Tryptophan / Q000032:analysis / Q000737:chemistry
D000596:Amino Acids; D013373:Substance P; D002244:Carbon; D014364:Tryptophan; D000803:Angiotensin I