[Applications of luminescence reactions in life science].
Bioluminescence and chemiluminescence have emerged in the last decade as a major tool for biochemical and biological studies. Several very sensitive assays have been developed in our laboratory such as enzymatic assays, immunoassays and detection of nucleic acids. The use of a new instrumentation allowing to dimensional photon counting has permit the emergence of new investigations at the cellular level. In addition to the advantages of sensitivity and the real-time non-invasive nature of this detection system, the imaging potential of using low-light and photon counting video cameras has been particularly influential in establishing its ascendence over more traditional systems. This review provides a reflection in this field through several applications in life sciences.
Nicolas
J C
JC
Centre de Recherches INSERM, Montpellier, France.
Boussioux
A M
AM
Joyeux
A
A
Balaguer
P
P
Térouanne
B
B
Roux
S
S
Gagne
D
D
Pons
M
M
D000255:Adenosine Triphosphate / Q000032:analysis; D007124:Immunoenzyme Techniques; D066298:In Vitro Techniques; D008163:Luminescent Measurements; D009249:NADP / Q000032:analysis; D015341:Nucleic Acid Probes; D017382:Reactive Oxygen Species / Q000032:analysis
D015341:Nucleic Acid Probes; D017382:Reactive Oxygen Species; D009249:NADP; D000255:Adenosine Triphosphate
Applications des réactions de luminescence dans le domaine des sciences de la vie.