title: Reg gene expression is increased in rat gastric enterochromaffin-like cells following water immersion stress.
abstract: OBJECTIVE
  Reg gene has been isolated from regenerating rat pancreatic islets, and subsequent studies have shown a trophic effect of Reg protein on islet cells. However, little is known about the role of Reg protein in the stomach. The aim of this study was to clarify the localization of Reg messenger RNA (mRNA) and its product in the stomach and to examine changes in the level of their expression during regeneration of gastric mucosal cells.
  
  
  METHODS
  Gastric lesions were experimentally induced in Sprague-Dawley rats by water immersion stress. Northern blot analysis and in situ hybridization studies were performed to examine changes in mRNA levels. Immunohistochemical studies were performed to identify the cellular localization and to investigate the change in Reg protein level.
  
  
  RESULTS
  Reg mRNA and its product were distributed in the basal part of the oxyntic mucosa and were expressed mainly in enterochromaffin-like cells. Levels of both Reg mRNA and its product were markedly increased in the water immersion-induced gastric lesions.
  
  
  CONCLUSIONS
  Reg mRNA and its product are expressed in gastric enterochromaffin-like cells, and their levels are increased during the healing process of water immersion-induced gastric lesions.
authors.affiliation: Division of Gerontology, Department of Internal Medicine, Kobe University School of Medicine, Kobe, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Asahara
authors.forename: S
authors.initials: S
authors.lastname: Mushiake
authors.forename: S
authors.initials: S
authors.lastname: Shimada
authors.forename: H
authors.initials: H
authors.lastname: Fukui
authors.forename: Y
authors.initials: Y
authors.lastname: Kinoshita
authors.forename: C
authors.initials: C
authors.lastname: Kawanami
authors.forename: T
authors.initials: T
authors.lastname: Watanabe
authors.forename: S
authors.initials: S
authors.lastname: Tanaka
authors.forename: A
authors.initials: A
authors.lastname: Ichikawa
authors.forename: Y
authors.initials: Y
authors.lastname: Uchiyama
authors.forename: Y
authors.initials: Y
authors.lastname: Narushima
authors.forename: S
authors.initials: S
authors.lastname: Takasawa
authors.forename: H
authors.initials: H
authors.lastname: Okamoto
authors.forename: M
authors.initials: M
authors.lastname: Tohyama
authors.forename: T
authors.initials: T
authors.lastname: Chiba
mesh_terms: D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002135:Calcium-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D004759:Enterochromaffin Cells / Q000378:metabolism*; D005753:Gastric Mucosa / Q000378:metabolism* / Q000473:pathology; D015870:Gene Expression; D007101:Immersion; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D051847:Lithostathine; D008297:Male; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013270:Stomach / Q000473:pathology; D013276:Stomach Ulcer / Q000209:etiology / Q000378:metabolism* / Q000473:pathology; D013312:Stress, Physiological / Q000503:physiopathology*
chemical_list: D002135:Calcium-Binding Proteins; D051847:Lithostathine; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; C497616:Reg1a protein, rat