Detection of canine erythrocytes infected with Babesia gibsoni by flow cytometry.
Peripheral blood samples from dogs infected with Babesia gibsoni were analyzed by flow cytometry for parasitized erythrocytes after staining with hydroethidine. Cells identified as positive by flow cytometry were erythrocytes infected with B. gibsoni. Analysis of 26 samples by flow cytometry for % parasitemia revealed a correlation coefficient of 0.97 in comparison to the conventional method of light microscopy.
Fukata
T
T
Department of Veterinary Medicine, College of Agriculture, Osaka Prefecture University, Japan.
Ohnishi
T
T
Okuda
S
S
Sasai
K
K
Baba
E
E
Arakawa
A
A
D000818:Animals; D001403:Babesia / Q000302:isolation & purification*; D001404:Babesiosis / Q000175:diagnosis*; D004283:Dog Diseases / Q000175:diagnosis*; D004285:Dogs; D004906:Erythrocyte Count / Q000662:veterinary; D004912:Erythrocytes / Q000469:parasitology*; D005434:Flow Cytometry / Q000662:veterinary; D005456:Fluorescent Dyes; D010617:Phenanthridines; D012156:Reticulocytes
D005456:Fluorescent Dyes; D010617:Phenanthridines; C058428:hydroethidine