Extracorporeal immunoadsorption from whole blood based on the avidin-biotin concept. Evaluation of a new method.
This study of 36 rats with rat colon adenocarcinoma transplants was carried out to investigate the efficacy of a new method of whole blood immunoadsorption (WBIA) in removing biotinylated monoclonal antibodies (MAbs) directly from unseparated blood, in order to increase 'the tumor/normal-tissue uptake ratio', as compared with extracorporeal immunoadsorption (ECIA) of antibodies from plasma. Compared with the ECIA system, the overall volume of the WBIA system (comprising only a pump, an adsorption column, a drop-chamber and tubings) was less (3.6 vs. 6.2 ml), and procedure duration 2 h less. The 17 rats undergoing the WBIA procedure, started 12 h after i.v. injection of 4.0-4.5 MBq 125I-BR96-biotin, manifested neither hemolysis nor any other complication; no signs of organ edema were found at dissection; whole body and blood radioactivity values were reduced by 51% and 89.5%, respectively. The WBIA method was as effective as ECIA, but technically simpler, safer and more reliable.
Garkavij
M
M
Department of Oncology, Lund University, Lund, Sweden.
Tennvall
J
J
Strand
S E
SE
Nilsson
R
R
Lindgren
L
L
Chen
J
J
Isaksson
M
M
Eriksson
H
H
Sjögren
H
H
D000230:Adenocarcinoma / Q000532:radiotherapy*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage / Q000097:blood* / Q000627:therapeutic use; D001360:Avidin; D001710:Biotin / Q000008:administration & dosage / Q000097:blood* / Q000627:therapeutic use; D003110:Colonic Neoplasms / Q000532:radiotherapy*; D004487:Edema / Q000209:etiology; D005112:Extracorporeal Circulation / Q000295:instrumentation / Q000379:methods; D006461:Hemolysis; D007163:Immunosorbent Techniques / Q000295:instrumentation; D007275:Injections, Intravenous; D007457:Iodine Radioisotopes / Q000008:administration & dosage / Q000627:therapeutic use; D008297:Male; D009368:Neoplasm Transplantation; D011851:Radioactivity; D016499:Radioimmunotherapy; D051381:Rats; D011914:Rats, Inbred BN; D012449:Safety; D013997:Time Factors; D014184:Transplantation, Homologous
C106558:125I-BR96-biotin; D000911:Antibodies, Monoclonal; D007457:Iodine Radioisotopes; D001360:Avidin; D001710:Biotin