title: Trisomy 12 is a rare cytogenetic finding in typical chronic lymphocytic leukemia.
abstract: We have studied 61 cases of B-chronic lymphocytic leukemia (CLL), combining cytological features, conventional cytogenetics and in situ hybridization (ISH). The comparison of these results constitutes the main subject of this study. The patients were cytologically classified according to the FAB criteria as: chronic lymphocytic leukemia (CLL) typical type (48 cases) and CLL atypical types (13 cases). Chromosome analysis was carried out on lymphoid cells from peripheral blood. The following mitogens were used: phytohemagglutinin (PHA) 5%, pokeweed (PWM) and lipopolysaccharide from E. coli. The ISH was performed with a biotin-labeled, chromosome 12-specific alpha satellite DNA probe, pSP12-1. Trisomy 12 was not found in any of the 48 patients with the typical type of CLL and in contradistinction it was present in some patients with atypical types. This study emphasizes the great importance of a closer link between hematological morphology and the cytogenetic approach.
authors.affiliation: Laboratori de Citologia Hematològica, Hospital Central L'Aliança, Barcelona, Spain.
authors.forename: S
authors.initials: S
authors.lastname: Woessner
authors.forename: F
authors.initials: F
authors.lastname: Solé
authors.forename: A
authors.initials: A
authors.lastname: Pérez-Losada
authors.forename: L
authors.initials: L
authors.lastname: Florensa
authors.forename: R M
authors.initials: RM
authors.lastname: Vilá
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D002881:Chromosomes, Human, Pair 12; D005069:Evaluation Studies as Topic; D005260:Female; D006801:Humans; D017403:In Situ Hybridization; D007621:Karyotyping; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000235:genetics* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D014314:Trisomy