Implications for the assay and biological activity of interleukin-4. Results of a WHO international collaborative study.
Five ampouled preparations of interleukin-4 (IL-4) have been evaluated by 36 laboratories in 14 countries for their suitability to serve as an international standard for this material in a joint international collaborative study for interleukin-3 (IL-3) and IL-4. The preparations were assayed in a wide range of in vitro bioassays and immunoassays. It is clear from the study that different recombinant preparations of IL-4 can have very different biological specific activities, including those from the same source (i.e., E. coli). In addition, immunoassay estimates of IL-4 levels did not correlate with those of bioassays, illustrating the fact that immunoassays do not necessarily measure biologically active cytokine. It is of interest that the estimates provided by the different bioassays were less variable than those produced by the immunoassays, suggesting that bioassays can be as accurate, if not more so, than immunoassays. The large reduction in the variability of estimates with the inclusion of a single reference preparation clearly illustrates the need for a single standard to assay IL-4. On the basis of the results reported here, with the agreement of the participants of the study and with the authorisation of the Expert Committee on Biological Standardization (ECBS) of the World Health Organization (WHO) the preparation of IL-4 (88/656) was established as the international standard for interleukin-4 with an assigned unitage of 1000 IU/ampoule.
Mire-Sluis
A R
AR
Division of Immunobiology, National Institute for Biological Standards and Control, South Mimms, Potters Bar, Herts, UK.
Das
R G
RG
Thorpe
R
R
D001681:Biological Assay; D002138:Calibration; D002460:Cell Line; D006801:Humans; D007118:Immunoassay; D007377:Interleukin-3 / Q000032:analysis; D015847:Interleukin-4 / Q000032:analysis* / Q000276:immunology
D007377:Interleukin-3; D015847:Interleukin-4