Urinary promutagens of smokers: comparison of concentration methods and relation to cigarette consumption.
Different extraction techniques can be used to concentrate the promutagens of cigarette smokers' urine before evaluation of their mutagenic potency by Ames test. In this study, three solid adsorbents, C18, XAD2 and CN were compared for their ability to concentrate the promutagens of smokers' urine prior to acetone elution. C18 extracts were observed to have a higher promutagenicity than XAD2 and CN extracts. The mutagenicity of smokers' urine depended on the smoking habits, and a strong correlation was observed between urinary promutagenicity, daily cigarette consumption, and the tobacco type (black or blond). Smokers of black tobacco had a higher urinary genotoxicity than smokers of blond tobacco, after taking into account the level of tobacco consumption. Urinary promutagenicity did not appear to depend on the tar level of cigarettes.
Kuenemann-Migeot
C
C
Laboratoire d'Hygiène et de Santé Publique, Faculté des Sciences Pharmaceutiques et Biologiques, Paris, France.
Callais
F
F
Momas
I
I
Festy
B
B
D003367:Cotinine / Q000032:analysis; D005260:Female; D006801:Humans; D016014:Linear Models; D008297:Male; D009152:Mutagenicity Tests; D009153:Mutagens / Q000032:analysis*; D009538:Nicotine / Q000032:analysis; D010947:Plants, Toxic; D010972:Platelet Activating Factor / Q000031:analogs & derivatives / Q000737:chemistry; D011137:Polystyrenes / Q000737:chemistry; D012117:Resins, Synthetic / Q000737:chemistry; D012486:Salmonella typhimurium / Q000187:drug effects; D012907:Smoking / Q000009:adverse effects / Q000652:urine*; D013638:Tars / Q000032:analysis; D014026:Nicotiana / Q000737:chemistry; D014556:Urine / Q000737:chemistry
D009153:Mutagens; D010972:Platelet Activating Factor; D011137:Polystyrenes; D012117:Resins, Synthetic; D013638:Tars; D009538:Nicotine; C026613:1-0-octadecyl 2-0-acetyl sn-glycero-3-phosphorylcholine; C003771:Amberlite XAD-2 resin; D003367:Cotinine