title: Establishment of a new extrahepatic bile duct carcinoma cell line, TFK-1.
abstract: A new human extrahepatic bile duct carcinoma cell line (TFK-1) was established from a surgically resected tumor specimen, which was histologically diagnosed as partly papillary adenocarcinoma and partly differentiated tubular adenocarcinoma. The tumor cells cultured in RPMI-1640 medium supplemented with 10% FBS grew as monolayers showing epithelial-like morphology with a population doubling time of 37 hr during exponential growth at passage 40. Chromosome number was distributed in the range of 72 to 76, with a modal number of 73. Tumor markers (CEA, CA19-9, ST-439, DUPAN-2) were negative in culture supernatant and plasma of SCID mice grafted with TFK-1 cells. Though no point mutation at 12 codon of K-ras was detected, expression of c-erb B-2 product and MUC1 antigen was positive. TFK-1 is the third cell line established from extrahepatic bile duct carcinomas in the world literature, and should provide useful information on various aspects of this type of neoplasm.
authors.affiliation: First Department of Surgery, Tohoku University School of Medicine, Sendai, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Saijyo
authors.forename: T
authors.initials: T
authors.lastname: Kudo
authors.forename: M
authors.initials: M
authors.lastname: Suzuki
authors.forename: Y
authors.initials: Y
authors.lastname: Katayose
authors.forename: M
authors.initials: M
authors.lastname: Shinoda
authors.forename: T
authors.initials: T
authors.lastname: Muto
authors.forename: K
authors.initials: K
authors.lastname: Fukuhara
authors.forename: T
authors.initials: T
authors.lastname: Suzuki
authors.forename: S
authors.initials: S
authors.lastname: Matsuno
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism / Q000473:pathology* / Q000648:ultrastructure; D000818:Animals; D001483:Base Sequence; D001650:Bile Duct Neoplasms / Q000378:metabolism / Q000473:pathology* / Q000648:ultrastructure; D017734:Bile Ducts, Extrahepatic / Q000378:metabolism / Q000473:pathology* / Q000648:ultrastructure; D014408:Biomarkers, Tumor / Q000378:metabolism; D002455:Cell Division; D004252:DNA Mutational Analysis; D005260:Female; D005434:Flow Cytometry; D011905:Genes, ras; D006801:Humans; D007150:Immunohistochemistry; D007621:Karyotyping; D008297:Male; D051379:Mice; D016513:Mice, SCID; D008875:Middle Aged; D008969:Molecular Sequence Data; D018396:Mucin-1 / Q000096:biosynthesis; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured
chemical_list: D014408:Biomarkers, Tumor; D018396:Mucin-1