Novel anthocyanins produced in petals of genetically transformed lisianthus.
The structures of the pigments in the deep purple flowers of a lisianthus line, transformed with a UDP-glucose:flavonoid-3-O-glucosyltransferase cDNA from Antirrhinum majus, have been studied using paper chromatography, HPLC and 1H and 13C NMR spectroscopy involving the use of 1H-1H COSY and TOCSY techniques. The transgenic line is shown to have produced the new anthocyanins delphinidin-3-O-beta-D-(6-O-alpha-L -rhamnopyranosylglucopyranoside)-5-O-beta-D-[6-E (and Z)-p-coumaroylglucopyranoside] and delphinidin -3-O-beta-D-glucopyranoside-5-O-beta-D-(6-O-E-p -coumaroylglucopyranoside) in addition to those found in the untransformed control plants.
Markham
K R
KR
New Zealand Institute for Industrial Research and Development, Lower Hutt, New Zealand.
D000872:Anthocyanins / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D018076:DNA, Complementary; D005964:Glucosyltransferases / Q000096:biosynthesis / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D015394:Molecular Structure; D030821:Plants, Genetically Modified; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000378:metabolism
D000872:Anthocyanins; D018076:DNA, Complementary; D011994:Recombinant Proteins; D005964:Glucosyltransferases; C023249:flavonol 3-O-glucosyltransferase