Isolation of a beta-tubulin gene from Fusarium moniliforme that confers cold-sensitive benomyl resistance.
A beta-tubulin gene from a UV-irradiated benomyl-resistant mutant of Fusarium moniliforme was isolated, cloned, and sequenced. The gene encodes a 446-amino-acid polypeptide with homology to other fungal beta-tubulins. RNA blot analysis showed expression of the gene during vegetative growth and conidial germination but no expression during conidiation. A point mutation, which likely confers benomyl resistance, has been identified in the cloned gene; this mutation results in a single amino acid substitution of asparagine for tyrosine at position 50. Expression of benomyl resistance in the mutant was also cold sensitive. Sexual crosses betweeen the mutant and a wild-type strain indicated cosegregation of benomyl resistance and cold sensitivity.
Yan
K
K
Department of Plant Pathology, University of Nebraska, Lincoln 68583-0722, USA.
Dickman
M B
MB
D000595:Amino Acid Sequence; D001483:Base Sequence; D001542:Benomyl / Q000494:pharmacology*; D003080:Cold Temperature; D004351:Drug Resistance; D005659:Fungicides, Industrial / Q000494:pharmacology*; D005670:Fusarium / Q000187:drug effects / Q000235:genetics*; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D009154:Mutation; D014404:Tubulin / Q000235:genetics*
D005659:Fungicides, Industrial; D014404:Tubulin; D001542:Benomyl