Induction of L-arginine-dependent production of nitric oxide in bovine monocytes by interferon gamma and lipopolysaccharide.
Bovine monocytes freshly isolated from peripheral blood were induced to produce nitric oxide by exposing them to recombinant bovine interferon gamma (rbIFN-y) and Escherichia coli lipopolysaccharide (LPS) in vitro. Moderate amounts of nitric oxide were induced by rbIFN-gamma alone, but larger amounts were induced by rbIFN-gamma and LPS together, the amount being dependent on the quantity of rbIFN-gamma added. Reactive nitrogen intermediates (RNI) were produced within six hours, their concentration peaked at four days and they were detectable for at least eight days after the cells had been stimulated with rbIFN-gamma and LPS. The production of RNI was diminished by the addition of NG-monomethyl-L-arginine. The data suggest that bovine monocytes can produce RNI via a pathway involving an inducible nitric oxide synthase.
Zhao
B Y
BY
University of Wisconsin-Madison 53706, USA.
Collins
M T
MT
Czuprynski
C J
CJ
D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives / Q000494:pharmacology*; D002417:Cattle; D002478:Cells, Cultured; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005260:Female; D007371:Interferon-gamma / Q000494:pharmacology*; D007700:Kinetics; D008070:Lipopolysaccharides / Q000494:pharmacology*; D009000:Monocytes / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D009569:Nitric Oxide / Q000097:blood*; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors; D011994:Recombinant Proteins; D013997:Time Factors; D019323:omega-N-Methylarginine
D004791:Enzyme Inhibitors; D008070:Lipopolysaccharides; D011994:Recombinant Proteins; D019323:omega-N-Methylarginine; D009569:Nitric Oxide; D007371:Interferon-gamma; D001120:Arginine; D019001:Nitric Oxide Synthase