Regulation of cell-cell contacts in developing Drosophila eyes by Dsrc41, a new, close relative of vertebrate c-src.
In Drosophila, Dsrc64 is considered a unique ortholog of the vertebrate c-src; however, we show evidence to the contrary. The closest relative of vertebrate c-src so far found in Drosophila is not Dsrc64, but Dsrc41, a gene identified for the first time here. In contrast to Dsrc64, overexpression of wild-type Dsrc41 caused little or no appreciable phenotypic change in Drosophila. Both gain-of-function and dominant-negative mutations of Dsrc41 caused the formation of supernumerary R7-type neurons, suppressible by one-dose reduction of boss, sev, Ras1, or other genes involved in the Sev pathway. Dominant-negative mutant phenotypes were suppressed and enhanced, respectively, by increasing and decreasing the copy number of wild-type Dsrc41. Colocalization of Dsrc41 protein, actin fibers and DE-cadherin, and Dsrc41-dependent disorganization of actin fibers and putative adherens junctions in precluster cells suggested that Dsrc41 may be involved in the regulation of cytoskeleton organization and cell-cell contacts in developing ommatidia.
Takahashi
F
F
Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Japan.
Endo
S
S
Kojima
T
T
Saigo
K
K
D000199:Actins / Q000032:analysis; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015820:Cadherins / Q000032:analysis; D002450:Cell Communication / Q000235:genetics; D003001:Cloning, Molecular; D004330:Drosophila / Q000235:genetics* / Q000254:growth & development; D029721:Drosophila Proteins; D005123:Eye / Q000166:cytology / Q000254:growth & development; D005136:Eye Proteins / Q000502:physiology; D018507:Gene Expression Regulation, Developmental; D017344:Genes, Insect / Q000235:genetics*; D016391:Genes, src / Q000235:genetics*; D008562:Membrane Glycoproteins / Q000502:physiology; D008969:Molecular Sequence Data; D009024:Morphogenesis; D009154:Mutation / Q000235:genetics; D017956:Photoreceptor Cells, Invertebrate / Q000254:growth & development*; D011505:Protein-Tyrosine Kinases; D016392:Proto-Oncogene Proteins pp60(c-src) / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics* / Q000502:physiology; D012333:RNA, Messenger / Q000032:analysis; D020794:Receptor Protein-Tyrosine Kinases / Q000502:physiology; D018000:Receptors, Peptide; D017422:Sequence Analysis, DNA; D014443:Tyrosine / Q000502:physiology; D014714:Vertebrates / Q000235:genetics
D000199:Actins; D015820:Cadherins; D029721:Drosophila Proteins; D005136:Eye Proteins; D008562:Membrane Glycoproteins; D012333:RNA, Messenger; D018000:Receptors, Peptide; C072141:boss protein, Drosophila; D014443:Tyrosine; D011505:Protein-Tyrosine Kinases; D020794:Receptor Protein-Tyrosine Kinases; C056737:sev protein, Drosophila; D016392:Proto-Oncogene Proteins pp60(c-src); C100382:Src42A protein, Drosophila