Effect of platelet-activating factor on cell differentiation of Trypanosoma cruzi.
The effects of platelet-activating factor (PAF), at (10)-6M and (10)-9M, on cell growth and cell differentiation of Trypanosoma cruzi were investigated. Cell differentiation was evaluated by both light and electron microscopy. At the concentrations used, PAF slightly interfered with the protozoan growth. However, parasites growth in the presence of PAF were significantly more differentiated than those grown in the absence of PAF, beginning on the fourth day of culture. A specific PAF receptor antagonist (WEB 2086) totally abrogated PAF effect on cell differentiation. These findings indicate that PAF triggers the process of cell differentiation in T. cruzi and suggest that these parasites have receptors for PAF.
Rodrigues
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Instituto de Microbiologia, Universidade Federal do Rio de Janeiro, Brazil.
Dutra
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Souto-Padrón
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Cordeiro
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Lopes
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D000818:Animals; D001381:Azepines / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D007700:Kinetics; D008854:Microscopy, Electron; D010972:Platelet Activating Factor / Q000494:pharmacology*; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology; D014230:Triazoles / Q000494:pharmacology; D014349:Trypanosoma cruzi / Q000187:drug effects* / Q000254:growth & development* / Q000648:ultrastructure
D001381:Azepines; D010972:Platelet Activating Factor; D010975:Platelet Aggregation Inhibitors; D014230:Triazoles; C052518:WEB 2086