Cloning and sequencing of cDNAs encoding the human sperm protein, Sp17.
In the present study we have cloned and sequenced two testis-specific cDNAs (1.3 kb and 1.6 kb) encoding a human sperm protein, designated HSp17. Each cDNA gave rise to identical protein sequences and differed only in the 5' untranslated region. The predicted amino-acid sequence revealed a protein of 17.5 kDa which exhibited a high degree of homology with both rabbit and mouse Sp17. Analysis of native and recombinant Sp17 by SDS-PAGE has shown the apparent molecular weight of the protein to be 24.5 kDa.
Lea
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IA
Department of Cell Biology and Anatomy, University of North Carolina at Chapel Hill 27599, USA. ialea@email.unc.edu
Richardson
R T
RT
Widgren
E E
EE
O'Rand
M G
MG
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry*; D006801:Humans; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D011506:Proteins / Q000737:chemistry / Q000235:genetics*; D011817:Rabbits; D013094:Spermatozoa / Q000737:chemistry*
D018076:DNA, Complementary; D011506:Proteins