title: Prevotella intermedia and Prevotella nigrescens serotypes, ribotypes and binding characteristics.
abstract: Type strains and 62 clinical isolates of Prevotella intermedia and Prevotella nigrescens were typed with the use of genomic DNA fingerprints and rRNA gene probes. The strains were further serotyped with monoclonal antibodies and characterized with SDS-PAGE, enzymatic activities, hemolysis and hemagglutination and coaggregation with Streptococcus and Actinomyces spp. P. intermedia and P. nigrescens were found to have distinct ribotype patterns which correspond to previously defined serotyupes I and II/III, respectively. No clear phenotypic difference related to hemolysis, hemagglutination and coaggregation with streptococcus and actinomyces species, or expression of aminopeptides and lipase was found between P. intermedia and P. migrescens.
authors.affiliation: Department of Oral Microbiology, Faculty of Odontology, University of Göteborg, Sweden.
authors.forename: G G
authors.initials: GG
authors.lastname: Dahlén
authors.forename: J R
authors.initials: JR
authors.lastname: Johnson
authors.forename: R
authors.initials: R
authors.lastname: Gmür
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion; D006384:Hemagglutination; D006461:Hemolysis; D006801:Humans; D066298:In Vitro Techniques; D010641:Phenotype; D018720:Prevotella / Q000145:classification* / Q000235:genetics* / Q000502:physiology; D018713:Prevotella intermedia / Q000145:classification* / Q000235:genetics* / Q000502:physiology; D012329:RNA, Bacterial / Q000235:genetics; D012335:RNA, Ribosomal / Q000235:genetics; D011817:Rabbits; D012703:Serotyping; D013045:Species Specificity
chemical_list: D012329:RNA, Bacterial; D012335:RNA, Ribosomal