One electron reduction of vanadate(V) to oxovanadium(IV) by low-molecular-weight biocomponents like saccharides and ascorbic acid: effect of oxovanadium(IV) complexes on pUC18 DNA and on lipid peroxidation in isolated rat hepatocytes.
One electron reduction of vanadate(V) to vanadyl(IV) occurs in the presence of biocomponents, such as, saccharide s and L-ascorbic acid under physiological conditions. The vanadyl(IV) complexes thus generated have been demonstrated to introduce nicks in pUC18 DNA in the absence or in the presence of hydrogen peroxide and also exhibit lipid peroxidation in isolated rat hepatocytes. Thus the reducing and complexing abilities of these molecules may help in understanding the in vivo reduction and the resultant toxicity to cells incubated with vanadate(V).
Sreedhara
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A
Department of Chemistry, Indian Institute of Technology, Powai, Bombay, India.
Susa
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N
Patwardhan
A
A
Rao
C P
CP
D000818:Animals; D001205:Ascorbic Acid / Q000737:chemistry / Q000494:pharmacology*; D002478:Cells, Cultured; D006601:Hexoses / Q000737:chemistry / Q000494:pharmacology*; D007700:Kinetics; D015227:Lipid Peroxidation / Q000187:drug effects*; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D009942:Organometallic Compounds / Q000737:chemistry / Q000494:pharmacology*; D010957:Plasmids / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis; D014638:Vanadates / Q000737:chemistry / Q000494:pharmacology*
D006601:Hexoses; D009942:Organometallic Compounds; D017392:Thiobarbituric Acid Reactive Substances; D014638:Vanadates; D001205:Ascorbic Acid