[Immunocytochemical method of assessment of the proliferative status of lymphoid cells in lymphoproliferative diseases].
Search for the new approaches to the diagnosis of lymphoproliferative diseases and transition to the classification scheme "Working Classification of Non-Hodgkin's Lymphomas for Clinical Use" involved assessment of the proliferative status of pathological lymphoid cells by immunocytochemical method with the use of DAKO-PC (Ki-67) monoclonal antibodies. The course and specific features of immunocytochemical reaction with the use of peroxidase-antiperoxidase immune complexes and complexes of alkaline phosphatase and monoclonal antibodies to it on various types of preparations are described in detail, this appreciably extending the potentialities of the use of this method. The studies were carried out with lymphoid cells of different organs of the immune system and peripheral blood of 44 patients with lymphoproliferative diseases and of 5 donors. The results permit considering the developed and tried immunocytochemical method for assessment of the proliferative status of pathological cell populations informative as regards the use of the "Working Classification of Non-Hodgkin's Lymphomas".
Malashenko
O S
OS
Samoĭlova
R S
RS
D000911:Antibodies, Monoclonal; D002455:Cell Division; D006801:Humans; D007124:Immunoenzyme Techniques; D008214:Lymphocytes / Q000166:cytology* / Q000276:immunology / Q000473:pathology; D008228:Lymphoma, Non-Hodgkin / Q000145:classification / Q000175:diagnosis / Q000276:immunology*
D000911:Antibodies, Monoclonal
Immunotsitokhimicheskiĭ metod otsenki proliferativnogo statusa limfoidnykh kletok pri limfoproliferativnykh zabolevaniiakh.