Hormonal and environmental regulation of a plant PDR5-like ABC transporter.
The PDR5 gene from yeast encodes an ABC (ATP-binding cassette) transporter involved in the ATP-dependent efflux of a variety of structurally unrelated cytotoxic compounds. We report here on the cDNA cloning and characterization of a PDR5 homolog (TUR2) from a higher eukaryote, the aquatic plant Spirodela polyrrhiza. We show that TUR2 transcripts accumulate throughout the plant following treatment with the steroid-like hormone, abscisic acid, and that this induction can be repressed by the adenine-derived hormone, kinetin. Furthermore, TUR2 gene expression is induced by environmental stress treatments such as low temperature and high salt. These data indicate that PDR5 homologs are present in plants, that they may function during stress conditions in an analogous fashion to that described in yeast, and that the expression of such ABC transporters is subject to a complex hormonal and environmental regulation.
Smart
C C
CC
Institute of Plant Sciences, Plant Biochemistry and Physiology, ETH Zürich, Universitätsstrasse 2, CH-8092 Zürich, Switzerland.
Fleming
A J
AJ
D018528:ATP-Binding Cassette Transporters / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D004777:Environment; D005656:Fungal Proteins / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000502:physiology; D008565:Membrane Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D010937:Plant Growth Regulators / Q000502:physiology*; D010940:Plant Proteins / Q000235:genetics*; D010944:Plants / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins; D017386:Sequence Homology, Amino Acid
D018528:ATP-Binding Cassette Transporters; D005656:Fungal Proteins; D008565:Membrane Proteins; C092438:PDR5 protein, S cerevisiae; D010937:Plant Growth Regulators; D010940:Plant Proteins; D012333:RNA, Messenger; D029701:Saccharomyces cerevisiae Proteins; C100878:TUR2 protein, Spirodela polyrrhiza