[Reversing effect of dimethyl-4,4'-dimethoxy-5,6,5'6'-dimethylenedioxybiphenyl-2,2'- dicarboxylate(DDB) on the phenotypes of human hepatocarcinoma cell line].
When human Bel-7402 hepatocarcinoma cell line grew in a medium containing 10(-4)M DDB, the secretion of alpha-fetoprotein (AFP) and the activity of gamma-glutamyl-transpeptidase (gamma-GT) were significantly lower than the control cells, whereas the albumin (ALB) secretion and the activity of tyrosine-alpha-ketoglutarate transaminase (TAT) were markedly increased. DDB at the concentration of 10(-4)M could significantly increase the content of cAMP in Bel-7402 cells, and also suppressed the expressions of oncogene c-myc and hepatocarcinoma marker AFP gene and enhanced the anti-oncogene p53 expression. The results of this paper suggest that DDB has some reversing effects on the phenotypes of human Bel-7402 hepatocarcinoma cell line.
Liu
Z
Z
Department II of Pharmacology, Chinese Academy of Medical Sciences, Beijing.
Liu
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G
Zhang
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S
D006528:Carcinoma, Hepatocellular / Q000473:pathology*; D002471:Cell Transformation, Neoplastic / Q000187:drug effects*; D004149:Dioxoles / Q000494:pharmacology*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D016259:Genes, myc; D016158:Genes, p53; D006801:Humans; D008113:Liver Neoplasms / Q000473:pathology*; D014407:Tumor Cells, Cultured / Q000378:metabolism; D000509:alpha-Fetoproteins / Q000378:metabolism; D005723:gamma-Glutamyltransferase / Q000378:metabolism
D004149:Dioxoles; D004365:Drugs, Chinese Herbal; D000509:alpha-Fetoproteins; C041398:7,7'-dimethoxy-(4,4'-bi-1,3-benzodioxole)-5,5'-dicarboxylic acid dimethyl ester; D005723:gamma-Glutamyltransferase