RcsC-mediated induction of colanic acid by secretion of streptokinase in Escherichia coli K-12.
The introduction of a plasmid containing skc (streptokinase-coding gene) fused with ompA signal sequence into Escherichia coli K-12 strains, rendered the bacteria mucoid. Measurement of the synthesis of beta-galactosidase from a cps-lacZ fusion (lacZ fusion to a gene necessary for capsule synthesis) showed that the mucoid phenotype was due to induction of the capsular polysaccharide colanic acid synthesis. The introduction of a plasmid carrying skc fused with malE (gene encoding maltose-binding protein) also induced cps-lacZ expression, but intracellular expression of streptokinase in E. coli did not. The cps expression by secretion of streptokinase was diminished to the basal level in a cps-lacZ strain carrying a rcsC mutation. These results show that the secretion of streptokinase in E. coli induces colanic acid synthesis through the RcsC-dependent pathway.
Lee
S H
SH
Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Yusong-gu, Taejon, South Korea.
Kim
I C
IC
Lee
W S
WS
Byun
S M
SM
D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D029968:Escherichia coli Proteins; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes; D010749:Phosphoprotein Phosphatases; D011134:Polysaccharides / Q000235:genetics*; D011135:Polysaccharides, Bacterial / Q000235:genetics; D011494:Protein Kinases; D013300:Streptokinase / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics*
D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D009097:Multienzyme Complexes; D011134:Polysaccharides; D011135:Polysaccharides, Bacterial; D014157:Transcription Factors; C489728:rcsC protein, E coli; C004275:colanic acid; D011494:Protein Kinases; D010749:Phosphoprotein Phosphatases; D013300:Streptokinase