Physical and biological effects of a surface coating procedure on polyurethane catheters.
Central venous catheters are widely used in clinical practice; however, complications such as venous thrombosis or infection are frequent. The physical and biological effects of a coating procedure designed to improve the blood-contacting properties of polyurethane central venous catheters (CVCs) were studied. The surface atomic composition of poly(vinyl pyrrolidone) (PVP)-coated or uncoated Pellethane single lumen CVCs was characterized by electron spectroscopy for chemical analysis (ESCA), which confirmed the presence of an oxygen-rich PVP layer on the former material. Topological analysis of both single and triple lumen CVCs by scanning force microscopy (SFM) revealed a very smooth surface in PVP-coated catheters compared to the more frequent surface irregularities found either in uncoated Pellethane or in four additional randomly selected, commercially available triple lumen polyurethane CVCs. The PVP-coated Pellethane showed a strong reduction in either fibrinogen or fibronectin adsorption compared to all other PVP-free polyurethane CVCs. This decreased protein adsorption led to a proportional reduction in protein-mediated adhesion of either Staphylococcus aureus or Staphylococcus epidermidis and in the binding of a monoclonal antibody directed against the cell-binding domain of fibronectin. Increased surface smoothness and hydrophilic properties of polyurethane CVCs might decrease the risk of bacterial colonization and infection.
Francois
P
P
Division of Infectious Diseases, University Hospital, Geneva, Switzerland.
Vaudaux
P
P
Nurdin
N
N
Mathieu
H J
HJ
Descouts
P
P
Lew
D P
DP
D000327:Adsorption; D000911:Antibodies, Monoclonal; D001422:Bacterial Adhesion; D002404:Catheterization / Q000592:standards*; D002405:Catheterization, Central Venous / Q000592:standards*; D004577:Electron Probe Microanalysis; D004797:Enzyme-Linked Immunosorbent Assay; D005353:Fibronectins / Q000276:immunology / Q000378:metabolism; D006801:Humans; D018625:Microscopy, Atomic Force; D011140:Polyurethanes / Q000737:chemistry*; D011205:Povidone / Q000737:chemistry*; D013203:Staphylococcal Infections / Q000517:prevention & control; D013211:Staphylococcus aureus / Q000378:metabolism; D013212:Staphylococcus epidermidis / Q000378:metabolism; D013499:Surface Properties
D000911:Antibodies, Monoclonal; D005353:Fibronectins; D011140:Polyurethanes; D011205:Povidone