title: Two different constituents of plasma increase cytosolic calcium selectively in neurons or glia of primary rat cerebellar cultures.
abstract: 1. The ability of several serum fractions to increase the cytosolic calcium concentration ([Ca2+]i) was tested in rat cerebellar cells maintained in primary culture. 2. Serum filtered through an ultrafiltration membrane with 3000 Da molecular mass cut-off (filtered serum, FS) selectively stimulated neurons whereas dialysed serum (DS) selectively stimulated glia. 3. The effects of FS were due to glutamate as they were reproduced by N-methyl-D-aspartate (NMDA), blocked by NMDA receptor antagonists and prevented by enzymatic removal of glutamate. 4. The effects of DS on glia were not reproduced by platelet-activating factor, thrombin or bradykinin. They were not lost on heating or extraction with diethyl ether. They were reproduced by a methanol-chloroform-HCl extract from DS and by several commercial fraction V plasma albumins. 5. These [Ca2+]i-increasing factors present in blood could contribute to brain damage during ischaemia if they reached the brain interstitium on disruption of the blood-brain barrier.
authors.affiliation: Universidad de Valladolid y Consejo Superior de Investigaciones Cientificas, Departamento de Bioquímica y Biología Molecular y Fisiología, Facultad de Medicina, Spain.
authors.forename: L
authors.initials: L
authors.lastname: Nuñez
authors.forename: J
authors.initials: J
authors.lastname: García-Sancho
mesh_terms: D000818:Animals; D001798:Blood Proteins / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D002531:Cerebellum / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D009457:Neuroglia / Q000187:drug effects* / Q000378:metabolism; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012709:Serum Albumin / Q000494:pharmacology*; D013997:Time Factors
chemical_list: D001798:Blood Proteins; D012709:Serum Albumin; D002118:Calcium