title: Fluorometric riboflavin titration in plasma by riboflavin-binding apoprotein as a method for vitamin B2 status assessment.
abstract: A method for plasma riboflavin determination by riboflavin-binding apoprotein titration has been proposed for vitamin B2 status evaluation. The method is based on the formation of riboflavin-apoprotein complex accompanied by full loss of fluorescence peculiar to free riboflavin. The data obtained have demonstrated a correlation with indicators of the vitamin B2 status such as urinary excretion, erythrocyte content, and stimulation of the glutathione reductase activity by flavin adenine dinucleotide. Levels > 6 ng/ml blood plasma may be considered to be a criterion for normal vitamin B2 supply.
authors.affiliation: Laboratory of Vitamin and Mineral Metabolism, Institute of Nutrition, Russian Academy of Medical Sciences, Moscow, Russia.
authors.forename: V M
authors.initials: VM
authors.lastname: Kodentsova
authors.forename: O A
authors.initials: OA
authors.lastname: Vrzhesinskaya
authors.forename: V B
authors.initials: VB
authors.lastname: Spirichev
mesh_terms: D000293:Adolescent; D000328:Adult; D001059:Apoproteins / Q000097:blood*; D002648:Child; D002675:Child, Preschool; D005260:Female; D005182:Flavin-Adenine Dinucleotide / Q000494:pharmacology; D006801:Humans; D008297:Male; D008875:Middle Aged; D009752:Nutritional Status; D012256:Riboflavin / Q000097:blood* / Q000652:urine; D013050:Spectrometry, Fluorescence
chemical_list: D001059:Apoproteins; D005182:Flavin-Adenine Dinucleotide; D012256:Riboflavin