title: A genetic linkage map of the Syrian hamster and localization of cardiomyopathy locus on chromosome 9qa2.1-b1 using RLGS spot-mapping.
abstract: The Syrian cardiomyopathic hamster (BIO14.6) has an inherited form of progressive myocardial necrosis and congestive heart failure. Although widely studied as an animal model for human hypertrophic cardiomyopathy, further genetic analysis has been limited by a scarcity of DNA markers. Until now, only six autosomal linkage groups have been described and the number of polymorphic loci was extremely limited. In this study, we applied the restriction landmark genome scanning (RLGS) spot-mapping method to construct a genetic map of the Syrian hamster (Mesocricetus auratus) using 72 back-cross progeny. Although the polymorphic rate is very low (3-7%) between the strains, 531 polymorphic spots/loci were mapped, showing the power of this approach and reasonable applicability to other organisms lacking a well-defined genetic map. Further, the spot markers which flank the cardiomyopathy (cm) locus were cloned to determine the chromosomal location of cm by fluorescent in situ hybridization (FISH) analysis, resulting in the assignment of the locus to the centromeric region of hamster chromosome 9qa2.1-b1. Several candidate genes responsible for hypertrophic cardiomyopathy in humans have been excluded.
authors.affiliation: Genome Science Laboratory, Tsukuba Life Science Center, The Institute of Physical and Chemical Research, Ibaraki, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Okazaki
authors.forename: H
authors.initials: H
authors.lastname: Okuizumi
authors.forename: T
authors.initials: T
authors.lastname: Ohsumi
authors.forename: O
authors.initials: O
authors.lastname: Nomura
authors.forename: S
authors.initials: S
authors.lastname: Takada
authors.forename: M
authors.initials: M
authors.lastname: Kamiya
authors.forename: N
authors.initials: N
authors.lastname: Sasaki
authors.forename: Y
authors.initials: Y
authors.lastname: Matsuda
authors.forename: M
authors.initials: M
authors.lastname: Nishimura
authors.forename: O
authors.initials: O
authors.lastname: Tagaya
authors.forename: M
authors.initials: M
authors.lastname: Muramatsu
authors.forename: Y
authors.initials: Y
authors.lastname: Hayashizaki
mesh_terms: D000818:Animals; D001483:Base Sequence; D009202:Cardiomyopathies / Q000235:genetics*; D002312:Cardiomyopathy, Hypertrophic / Q000235:genetics; D002874:Chromosome Mapping; D006224:Cricetinae; D003433:Crosses, Genetic; D017931:DNA Primers; D005260:Female; D008040:Genetic Linkage; D006333:Heart Failure / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008647:Mesocricetus / Q000235:genetics*; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D015183:Restriction Mapping
chemical_list: D017931:DNA Primers