Inhibitory mechanisms of H(+)-ATPase inhibitor bafilomycin A1 and carbonic anhydrase II inhibitor acetazolamide on experimental bone resorption.
The effects of the vacuolar-type H(+)-ATPase inhibitor bafilomycin A1 (baf.A1) and the carbonic anhydrase II inhibitor acetazolamide (AZ) on bone resorption and procathepsin L secretion of rat osteoclasts were investigated using the bone slice assay method, pit formation test. Baf.A1 completely suppressed osteoclastic bone resorption stimulated by parathyroid hormone (PTH), but did not affect procathepsin L secretion, while AZ suppressed both bone resorption and procathepsin L secretion. These findings suggest that bone resorption by procathepsin L secretion and its processing are regulated by proton production and proton secretion.
Ohba
Y
Y
Department of Orthodontics, School of Dentistry, Tokushima University, Japan.
Ohba
T
T
Sumitani
K
K
Tagami-Kondoh
K
K
Hiura
K
K
Miki
Y
Y
Kakegawa
H
H
Takano-Yamamoto
T
T
Katunuma
N
N
D000086:Acetazolamide / Q000494:pharmacology*; D000251:Adenosine Triphosphatases / Q000037:antagonists & inhibitors*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001862:Bone Resorption / Q000378:metabolism*; D002257:Carbonic Anhydrase Inhibitors / Q000494:pharmacology*; D056668:Cathepsin L; D002403:Cathepsins / Q000378:metabolism*; D004792:Enzyme Precursors / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D018942:Macrolides; D010010:Osteoclasts / Q000187:drug effects / Q000378:metabolism; D010281:Parathyroid Hormone / Q000494:pharmacology; D017494:Proton Pumps / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D025262:Vacuolar Proton-Translocating ATPases
D000900:Anti-Bacterial Agents; D002257:Carbonic Anhydrase Inhibitors; D004792:Enzyme Precursors; D018942:Macrolides; D010281:Parathyroid Hormone; D017494:Proton Pumps; C040929:bafilomycin A1; D002403:Cathepsins; C063905:procathepsin L; D056668:Cathepsin L; D000251:Adenosine Triphosphatases; D025262:Vacuolar Proton-Translocating ATPases; D000086:Acetazolamide