[Study of pharmacokinetics of liver targeting antimalarial agent neoglycoalbumin-primaquine conjugate (NGA-PQ) and primaquine phosphate in mouse].
A normal phase high-performance liquid chromatography process was used to separate and detect primaquine in blood and liver after a single intravenous dose of the hepatic targeting agent neoglycoalbumine-primaquine conjugate (NGA-PQ) and primaquine phosphate (PQP) in mice. 6-Methoxy-8-(4-amino-butyrylamino) quinoline synthesized and identified by us was used as an internal standard to be added to biologic samples obtained from mice at different times after given NGA-PQ or PQP. The mixture was extracted with ether after alkalinization in the PQP group. In the NGA-PQ group, the biological samples must be hydrolized by heating under nitrogen and acid condition in a domestic pressure cooker before extraction. The extracts were evaporated to dryness under nitrogen, then dissolved in the mobile phase (chloroform-methanol-amonium hydroxide = 86.8: 12.5: 0.7). The results showed that the hepatic PQ collecting ratio and the retention time of PQ in liver in the NGA-PQ group were higher and longer than those in the PQP group. The results also point out that NGA-PQ has liver targeting property.
Li
T L
TL
College of Pharmacy, West China University of Medical Sciences, Chengdu.
Pang
Q J
QJ
He
Y L
YL
Wang
P
P
D000418:Albumins / Q000493:pharmacokinetics*; D000818:Animals; D000962:Antimalarials / Q000493:pharmacokinetics*; D004337:Drug Carriers; D008099:Liver / Q000378:metabolism*; D051379:Mice; D011319:Primaquine / Q000493:pharmacokinetics*
D000418:Albumins; D000962:Antimalarials; D004337:Drug Carriers; D011319:Primaquine