Detection of Taenia saginata antigens in faeces by ELISA.
Stool samples were examined by capture ELISA for the presence of T. saginata antigens. The specimens were from 303 patients infected with T. saginata, 43 samples from individuals suffering from bacterial, fungal, protozoan and nematode infections and 36 ones deriving from uninfected persons. The samples were diluted 1:10 (w:v) in PBS + 0.35% Tween 20, centrifuged twice at 5000 g for 20 min at room temperature and resulting supernatant at 12,000 g for 30 min at 0 degree C. The capturing and detecting polyclonal antibodies were prepared in rabbits immunizing them with T. saginata surface antigens. The stool samples freshly obtained, stored at 4 degrees C not longer than 72 h, or kept at at -20 to -30 degrees C even for 24 months showed reliable results in ELISA. The samples kept for a long time at 4 degrees C, dried and covered with moult were negative in the test. All samples taken from patients infected with other than T. saginata parasites or uninfeced were negative.
Machnicka
B
B
Institute of Parasitology of the Polish Academy of Sciences, Warsaw, Poland.
Dziemian
E
E
Zwierz
C
C
D000818:Animals; D000947:Antigens, Helminth / Q000032:analysis*; D003551:Cysticercosis / Q000175:diagnosis / Q000276:immunology* / Q000469:parasitology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005243:Feces / Q000469:parasitology*; D006801:Humans; D013621:Taenia / Q000276:immunology / Q000302:isolation & purification*
D000947:Antigens, Helminth