[Expression of human immunodeficiency virus gag antigens and formation of virus-like particles in a cell culture, infected with recombinant vaccinia virus strains (an electron-microscopic study)].
The synthesis of gag antigens of human immunodeficiency virus (HIV) by recombinant vaccinia virus strains containing the expressed genes gag and gag-pol and the capacity of these proteins to formation of virus-like particles during infection of various cell cultures were studied. The recombinant strain containing the truncated gag gene (p48gag) was shown to effectively synthesize gag polypeptides and to form immature virus-like particles during infection of all the cell cultures tested (CV-1, Hep-2, HT-29). The morphogenesis of mature virus-like particles was detected by electron microscopy only in infection of Hep-2 cells with a strain containing a complete gag-pol sequence of HIV.
Grigor'ev
V B
VB
Lideman
L F
LF
Gibadulin
R A
RA
Sazykin
A Iu
AIu
Klimenko
S M
SM
D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D015685:Fusion Proteins, gag-pol / Q000235:genetics; D015683:Gene Products, gag / Q000235:genetics*; D015497:HIV-1 / Q000235:genetics* / Q000276:immunology; D006801:Humans; D008854:Microscopy, Electron; D011995:Recombination, Genetic; D014407:Tumor Cells, Cultured; D014616:Vaccinia virus / Q000235:genetics*; D014771:Virion
D015685:Fusion Proteins, gag-pol; D015683:Gene Products, gag
Ekspressiia gag-antigenov virusa immunodefitsita cheloveka i obrazovanie virusopodobnykh chastits v kul'ture kletok, infitsirovannykh rekombinantnymi shtammami virusa ospovaktsiny (élektronno-mikroskopicheskoe issldovanie).