Molecular cloning of a mouse homologue for the Drosophila splicing regulator Tra2.
We report the identification of a mouse cDNA, SIG41, encoding a protein of 288 amino acids that is 45% identical (58% similar) to the Drosophila splicing regulator Tra2. SIG41 cDNA contains four polyadenylation signals whose alternative use gives rise to four types of transcripts (2.1, 2.0, 1.5, and 1.4 kb) in mouse cells. Northern analysis and RT-PCR assays showed that SIG41 mRNA is present in virtually all the cell lines and tissues studied, with remarkable levels of expression in uterus and brain tissues. Differential stability of the SIG41 mRNAs was detected in mouse macrophage cells.
Segade
F
F
Departamento de Bioquímica y Biología Molecular, Universidad de Oviedo, Spain.
Hurlé
B
B
Claudio
E
E
Ramos
S
S
Lazo
P S
PS
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D017931:DNA Primers; D018076:DNA, Complementary; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000378:metabolism; D015870:Gene Expression; D051379:Mice; D008969:Molecular Sequence Data; D009687:Nuclear Proteins; D012326:RNA Splicing; D012333:RNA, Messenger; D016601:RNA-Binding Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D012261:Ribonucleoproteins / Q000737:chemistry / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D000068103:Serine-Arginine Splicing Factors
D017931:DNA Primers; D018076:DNA, Complementary; D029721:Drosophila Proteins; D009687:Nuclear Proteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D012261:Ribonucleoproteins; C100345:Tra2b protein, mouse; C066174:tra2 protein, Drosophila; D000068103:Serine-Arginine Splicing Factors