title: Developmental regulation of collagen differential expression in the rabbit bladder.
abstract: OBJECTIVE
  It has been suggested that the amount and relative ratios of different types of collagen influence bladder compliance. To understand the mechanisms regulating collagen synthesis and degradation in the bladder we investigated the gene expression of collagen types I and III in the rabbit bladder during normal development.
  
  
  METHODS
  New Zealand white rabbits ages fetus to adult were used for this study. The mid portion of the bladder wall was harvested. Northern blot hybridization was performed to analyze quantitatively collagen types I and III messenger (m) ribonucleic acid (RNA), and in situ hybridization was done to localize the messages. Corresponding protein distributions were obtained by immunohistochemical staining.
  
  
  RESULTS
  Types I and III collagen are developmentally regulated at the level of gene expression. The temporal and spatial distribution of the mRNA transcripts of alpha 1(I) and alpha 1(III) correlates with extracellular protein deposition. Differential distribution of the mRNA transcripts is also developmentally regulated.
  
  
  CONCLUSIONS
  This study characterizes the relationship between collagen gene expression and normal rabbit bladder development. The expression of alpha 1(I) and alpha 1(III) alters as the bladder grows. High levels and nonconcordant up and down regulation of different types of collagen mRNA during early development demonstrate the complexity of the extracellular matrix in a young bladder. This observation may be important in our understanding of the injury response in a developing versus mature bladder.
authors.affiliation: Division of Pediatric Urology, Children's Hospital and Medical Center, University of Washington, Seattle, USA.
authors.forename: L Y
authors.initials: LY
authors.lastname: Yao
authors.forename: S
authors.initials: S
authors.lastname: Tekgul
authors.forename: K K
authors.initials: KK
authors.lastname: Kim
authors.forename: H
authors.initials: H
authors.lastname: Li
authors.forename: M E
authors.initials: ME
authors.lastname: Mitchell
authors.forename: M C
authors.initials: MC
authors.lastname: Carr
mesh_terms: D000818:Animals; D003094:Collagen / Q000096:biosynthesis* / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D012333:RNA, Messenger / Q000032:analysis; D011817:Rabbits; D001743:Urinary Bladder / Q000033:anatomy & histology / Q000254:growth & development / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D003094:Collagen