Direct transformation of Neisseria gonorrhoeae by gel-isolated DNA.
The naturally competent organism, Neisseria gonorrhoeae, can efficiently transform a marker carried on DNA purified in low-melting-temperature agarose without prior purification or dilution. Neither the agarose or buffer components inhibit transformation frequencies, but exposure to UV irradiation completely abrogates transformation.
Pritchard
K H
KH
Department of Microbiology-Immunology, Northwestern University Medical School, Chicago, IL 60611, USA.
Seifert
H S
HS
D004587:Electrophoresis, Agar Gel; D009344:Neisseria gonorrhoeae / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D014169:Transformation, Bacterial; D014466:Ultraviolet Rays