The major fimbrial subunit of Bordetella pertussis binds to sulfated sugars.
Bordetella pertussis fimbriae are composed of major and minor subunits, and recently it was shown that the minor fimbrial subunit binds to Vla-5, a receptor located on monocytes (W. Hazenbos, C. Geuijen, B. van den Berg, F. Mooi, and R. van Furth, J. Infect. Dis. 171:924-929, 1995). Here we present evidence that the major subunits bind to sulfated sugars, which are ubiquitous in the respiratory tract. Binding was observed to chondroitin sulfate, heparan sulfate, and dextran sulfate but not to dextran. Removal of the minor subunit from fimbriae did not significantly affect binding to sulfated sugars, indicating that the major subunit alone is sufficient for this binding. Fimbriae were also able to bind HEp-2 cells, which are known to display glycoconjugates on their surface. This binding was not dependent on the presence of the minor subunit. However, binding was dependent on the sulfation state of the glycoconjugates, since inhibition of the sulfation resulted in a significant reduction of fimbria binding. The specificity of fimbria binding was further characterized by using heparan sulfate-derived disaccharides in inhibition assays. Two disaccharides were highly effective inhibitors, and it was observed that both the degree of sulfation and the arrangement of the sulfate groups on the disaccharides were important for binding to fimbriae. B. pertussis bacteria also bound to sulfated sugars and HEp-2 cells, and analysis of B. pertussis mutants indicated that both filamentous hemagglutinin and fimbriae were required for this binding. A host protein present in the extracellular matrix, fibronectin, has binding activities similar to those of B. pertussis fimbriae, binding to both Vla-5 and sulfated sugars. Two regions in the major fimbrial subunit were identified which showed similarity with fibronectin peptides which bind to sulfated sugars. Thus, B. pertussis fimbriae exemplify molecular mimicry and may co-opt host processes by mimicking natural ligand-receptor interactions.
Geuijen
C A
CA
Molecular Microbiology Unit, National Institute of Health and Environmental Protection, The Netherlands.
Willems
R J
RJ
Mooi
F R
FR
D000595:Amino Acid Sequence; D001422:Bacterial Adhesion / Q000502:physiology; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001886:Bordetella pertussis / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity; D050260:Carbohydrate Metabolism; D002240:Carbohydrate Sequence; D002460:Cell Line; D002809:Chondroitin Sulfates / Q000378:metabolism; D016264:Dextran Sulfate / Q000378:metabolism; D004187:Disaccharides / Q000737:chemistry / Q000378:metabolism; D005353:Fibronectins / Q000235:genetics / Q000378:metabolism; D010861:Fimbriae, Bacterial / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D006497:Heparan Sulfate / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D013431:Sulfates / Q000378:metabolism
D001426:Bacterial Proteins; D002809:Chondroitin Sulfates; D016264:Dextran Sulfate; D004187:Disaccharides; D005353:Fibronectins; D006497:Heparan Sulfate; D013431:Sulfates