Inhibition of aldose reductase by dihydroflavonols in Engelhardtia chrysolepis and effects on other enzymes.
Astilbin and neoastilbin, dihydroflavonol rhamnosides from Engelhardtia chrysolepis, showed potent inhibition of lens aldose reductase. Kinetic analysis showed astilbin exhibited uncompetitive inhibition against both dl-glyceraldehyde and NADPH. These taxifolin glycosides were selective inhibitors of aldose reductase with no inhibition of NADH oxidase.
Haraguchi
H
H
Faculty of Engineering, Fukuyama University, Japan.
Ohmi
I
I
Masuda
H
H
Tamura
Y
Y
Mizutani
K
K
Tanaka
O
O
Chou
W H
WH
D000449:Aldehyde Reductase / Q000037:antagonists & inhibitors*; D000818:Animals; D002417:Cattle; D004365:Drugs, Chinese Herbal; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005419:Flavonoids / Q000494:pharmacology*; D044948:Flavonols; D005985:Glyceraldehyde / Q000494:pharmacology; D007668:Kidney / Q000201:enzymology*; D007700:Kinetics; D007908:Lens, Crystalline / Q000201:enzymology*; D008930:Mitochondria, Liver / Q000201:enzymology; D009097:Multienzyme Complexes / Q000032:analysis; D009247:NADH, NADPH Oxidoreductases / Q000032:analysis; D009249:NADP / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry; D010944:Plants / Q000737:chemistry*; D011794:Quercetin / Q000494:pharmacology; D013552:Swine
D004365:Drugs, Chinese Herbal; D004791:Enzyme Inhibitors; D005419:Flavonoids; D044948:Flavonols; D009097:Multienzyme Complexes; C099069:astilbin; D005985:Glyceraldehyde; D009249:NADP; D011794:Quercetin; D000449:Aldehyde Reductase; C023107:NADH oxidase; D009247:NADH, NADPH Oxidoreductases