A new set of primers for the detection of Toxoplasma gondii in amniotic fluid using polymerase chain reaction.
A new PCR system including a pair of primers, a probe and an internal control were designed from the B1 gene of Toxoplasma gondii. The system described allowed the detection of less than 10 tachyzoites of the RH strain of T. gondii. Among 21 amniotic fluid samples, this system diagnosed the cases of congenital toxoplasmosis which were simultaneously diagnosed using mice inoculation, in vitro culture, and serology from both amniotic fluid and fetal blood. These results show that these new primers allow for a highly sensitive detection of T. gondii DNA.
Pelloux
H
H
Département de Parasitologie-Myocologie Médicale et Moléculaire, Faculté de Médecine, Université Joseph Fourier, Grenoble, France.
Weiss
J
J
Simon
J
J
Muet
F
F
Fricker-Hidalgo
H
H
Goullier-Fleuret
A
A
Ambroise-Thomas
P
P
D000653:Amniotic Fluid / Q000469:parasitology*; D000818:Animals; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics*; D016054:DNA, Protozoan / Q000235:genetics* / Q000302:isolation & purification; D003951:Diagnostic Errors; D005260:Female; D005312:Fetal Blood / Q000469:parasitology; D017125:Genes, Protozoan; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000706:statistics & numerical data; D011247:Pregnancy; D012680:Sensitivity and Specificity; D014122:Toxoplasma / Q000235:genetics* / Q000302:isolation & purification*; D014125:Toxoplasmosis, Congenital / Q000175:diagnosis / Q000469:parasitology
D017931:DNA Primers; D016054:DNA, Protozoan