title: Relationships between antibodies against human soluble complement receptor 1 (hsCR1) from various species.
abstract: The relationships between antibodies against human soluble complement receptor 1 (hsCR1) were studied in rodents, dogs, nonhuman primates, and humans. An antibody response occurred in all species except humans. The anti-hsCR1 antibodies from the various species were characterized to determine if they recognize similar epitopes on the hsCR1 molecule. Dog and monkey sera, positve for hsCR1 binding, were used as blocking antibodies against mouse anti-hsCR1 monoclonal antibodies as well as mouse and rat anti-hsCR1-positive sera. Human sera (blood group antisera: anti-Knops, anti-McCoy, anti-Knops/McCoy, anti-Swain-Langley) and serum from one burn patient (who became seropositive despite ever receiving treatment with hsCR1) were also used to test blocking of mouse, rat, dog, and monkey anti-hsCR1. Characterization of anti-hsCR1 antibodies from different species demonstrated that hsCR1 causes divergent antibody responses among animals. While mouse, rat, and dog antibodies cross inhibit binding by approximately 50%, monkey antibodies recognize primarily different epitopes of the hsCR1 molecule. Moreover, human antibodies binding hsCR1 are completely different from the animal antibodies, including monkey. This study indicates that although hsCR1 is immunogenic in animals, there is a difference in response between species, particularly between nonprimates and primates, and finally, that this antibody response is not predictive for humans.
authors.affiliation: SmithKline Beecham Pharmaceuticals, King of Prussia, Pennsylvania 19406-0939, USA.
authors.forename: E V
authors.initials: EV
authors.lastname: Ruggieri
authors.forename: P J
authors.initials: PJ
authors.lastname: Bugelski
authors.forename: J M
authors.initials: JM
authors.lastname: Kaplan
authors.forename: D
authors.initials: D
authors.lastname: Everitt
authors.forename: J
authors.initials: J
authors.lastname: Lipani
authors.forename: D K
authors.initials: DK
authors.lastname: Jorkasky
authors.forename: S C
authors.initials: SC
authors.lastname: Boike
authors.forename: F
authors.initials: F
authors.lastname: DeClement
authors.forename: F D
authors.initials: FD
authors.lastname: Moore
authors.forename: D J
authors.initials: DJ
authors.lastname: Herzyk
mesh_terms: D000818:Animals; D000906:Antibodies / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification; D000915:Antibody Affinity / Q000276:immunology*; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology; D005260:Female; D006801:Humans; D008252:Macaca fascicularis; D008297:Male; D051379:Mice; D051381:Rats; D011951:Receptors, Complement / Q000276:immunology*; D011994:Recombinant Proteins / Q000276:immunology; D013045:Species Specificity
chemical_list: D000906:Antibodies; D000939:Epitopes; D011951:Receptors, Complement; D011994:Recombinant Proteins