Mosaic structures of neurotoxins produced from Clostridium botulinum types C and D organisms.
We isolated the gene encoding a botulinum neurotoxin (BoNT) of 1285 amino acids with a molecular weight of 147,364 from the toxigenic bacteriophage d-sA of Clostridium botulinum type D strain South African (Dsa). The BoNT of Dsa (BoNT/Dsa) is composed of three regions on the basis of the homology to BoNT types C1 (BoNT/C1) and D (BoNT/D). The N-terminal (Met-1 to Val-522) and the C-terminal regions (Trp-945 to Glu-1285) have high identity to corresponding regions of BoNT/D (96% identity) and BoNT/C1 (74% identity), respectively. The core region (Pro-523 to Lys-944) is common to three toxins (83% to 92% identity). These results suggest that neurotoxins produced from Clostridium botulinum types C and D are composed in a mosaic-like fashion.
Moriishi
K
K
Department of Veterinary Science, National Institute of Health, Tokyo, Japan. kohji@nih.go.jp
Koura
M
M
Abe
N
N
Fujii
N
N
Fujinaga
Y
Y
Inoue
K
K
Ogumad
K
K
D000595:Amino Acid Sequence; D001905:Botulinum Toxins / Q000235:genetics*; D003001:Cloning, Molecular; D003014:Clostridium botulinum / Q000378:metabolism*; D008969:Molecular Sequence Data; D009030:Mosaicism; D017386:Sequence Homology, Amino Acid
D001905:Botulinum Toxins