Regional expression and subcellular localization of the tyrosine-specific phosphatase SH-PTP2 in the adult human nervous system.
The protein tyrosine phosphatase SH-PTP2 has been implicated in a variety of cell signaling cascades, including those mediating neuronal survival. We therefore investigated the expression of SH-PTP2 in the adult human nervous system using Western blotting, immunohistochemistry and immunoelectron microscopy. SH-PTP2 immunoreactivity was noted only in neurons, but was not restricted to a specific neuronal type or location. Immunohistochemistry showed perikaryal staining, whereas Western blotting and ultrastructural analysis suggested that SH-PTP2 is present in axons as well. While immunohistochemistry showed a Nissl-like pattern in large motor neurons, immunoelectron microscopy demonstrated a diffuse pattern of cytoplasmic staining, without apparent preferential localization. The presence of the SH2 domain-containing tyrosine-specific phosphatase SH-PTP2 in diverse neurons in the adult nervous system suggests that SH-PTP2 may play a role in a broad spectrum of neuronal responses.
Reeves
S A
SA
Molecular Neuro-Oncology Laboratory, Massachusetts General Hospital, Charlestown, USA.
Ueki
K
K
Sinha
B
B
Difiglia
M
M
Louis
D N
DN
D000328:Adult; D015153:Blotting, Western; D001921:Brain / Q000166:cytology / Q000201:enzymology*; D006801:Humans; D007150:Immunohistochemistry; D047908:Intracellular Signaling Peptides and Proteins; D008854:Microscopy, Electron; D009474:Neurons / Q000201:enzymology / Q000648:ultrastructure; D054592:Protein Tyrosine Phosphatase, Non-Receptor Type 11; D053824:Protein Tyrosine Phosphatase, Non-Receptor Type 6; D017027:Protein Tyrosine Phosphatases / Q000032:analysis*; D054593:SH2 Domain-Containing Protein Tyrosine Phosphatases; D013116:Spinal Cord / Q000166:cytology / Q000201:enzymology*
D047908:Intracellular Signaling Peptides and Proteins; C516724:PTPN11 protein, human; C509249:PTPN6 protein, human; D054592:Protein Tyrosine Phosphatase, Non-Receptor Type 11; D053824:Protein Tyrosine Phosphatase, Non-Receptor Type 6; D017027:Protein Tyrosine Phosphatases; D054593:SH2 Domain-Containing Protein Tyrosine Phosphatases