Mapping of type I loci from human chromosome 7 reveals segments of conserved synteny on pig chromosomes 3, 9, and 18.
We have mapped in the pig (Sus scrofa) the genes for zona pellucida glycoprotein 3 (ZP3), erythropoietin (EPO), and malate dehydrogenase 2 (MDH2) by somatic cell hybrid analysis in the pig genome. Previously, the gene for the T-cell receptor beta cluster (TCRB) was assigned to SSC 18 and that for interleukin 6 (IL6) to SSC 9. However, statistical analysis of mapping data for ZP3, EPO, and MDH2 did not discriminate between SSC 9 and SSC 3. Porcine-specific PCR primers for ZP3 and IL6 were used to isolate probes from porcine YAC libraries. These two genes were mapped by FISH to porcine chromosome regions 3pter --> p15 (ZP3) and 9q14 --> q15 (IL6). Comparative mapping considerations permit the tentative assignment of porcine EPO and MDH2 to 3pter --> p15.
Bruch
J
J
Abteilung Medizinische Genetik, Universität Ulm, Germany.
Rettenberger
G
G
Leeb
T
T
Meier-Ewert
S
S
Klett
C
C
Brenig
B
B
Hameister
H
H
D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D002897:Chromosomes, Human, Pair 7; D017124:Conserved Sequence; D017931:DNA Primers; D006801:Humans; D006822:Hybrid Cells; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D013552:Swine / Q000235:genetics*
D017931:DNA Primers