title: Analysis and comparison of the mouse and human immunoglobulin heavy chain JH-Cmu-Cdelta locus.
abstract: We report here 23,686 bases of contiguous DNA sequences from the mouse germline immunoglobulin heavy chain (H) constant (C) mu delta region. The sequence spans the joining (JH) regions, the mu constant region (C mu), the delta constant region (C delta) coding regions, a domain relic, the mu switch region (S mu), seven blocks of simple sequence repeats, a large unique sequence inverted repeat, a large unique sequence forward repeat, and all of the intervening material. A comparison of this 23.7-kb region with the corresponding human C mu/C delta region reveals clear homology in the coding and introns of C mu but not in the 5' flanking J gene segments nor in the intergenic and C delta regions. This mixed pattern of similarity between the human and the mouse sequences contrasts with high levels of similarity found in the T-cell receptor C alpha/C delta region and alpha and beta myosin genes and the very low levels found in the gamma-crystallin, XRCC1, and beta-globin gene clusters. The human and mouse comparison further suggests the incorporation of novel sequences into expressed genes of IgD.
authors.affiliation: Center for Environmental Health-Biology, University of Victoria, British Columbia, Canada.
authors.forename: B F
authors.initials: BF
authors.lastname: Koop
authors.forename: J E
authors.initials: JE
authors.lastname: Richards
authors.forename: T D
authors.initials: TD
authors.lastname: Durfee
authors.forename: J
authors.initials: J
authors.lastname: Bansberg
authors.forename: J
authors.initials: J
authors.lastname: Wells
authors.forename: A C
authors.initials: AC
authors.lastname: Gilliam
authors.forename: H L
authors.initials: HL
authors.lastname: Chen
authors.forename: A
authors.initials: A
authors.lastname: Clausell
authors.forename: P W
authors.initials: PW
authors.lastname: Tucker
authors.forename: F R
authors.initials: FR
authors.lastname: Blattner
mesh_terms: D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D004247:DNA / Q000235:genetics*; D005803:Genes, Immunoglobulin; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D007144:Immunoglobulin J-Chains / Q000235:genetics; D007138:Immunoglobulin delta-Chains / Q000235:genetics; D007148:Immunoglobulin mu-Chains / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D012689:Sequence Homology, Nucleic Acid
chemical_list: D007143:Immunoglobulin Heavy Chains; D007144:Immunoglobulin J-Chains; D007138:Immunoglobulin delta-Chains; D007148:Immunoglobulin mu-Chains; D004247:DNA