title: Characterization of a cDNA encoding murine coagulation factor VII.
abstract: The cDNA encoding murine coagulation factor VII (mfVII) was isolated and reconstructed from a lambda Zap cDNA library generated from murine liver mRNA. The cDNA contains 1903 nucleotides spanning 15 bases upstream of the 5'-translation initiation codon, an open reading frame of 1338 nucleotides, 550 nucleotides downstream of the first stop codon and a 3' poly(A) tail. The translation product is composed of a 41-amino acid signal/propeptide region followed by a 405-residue mature protein. The latter is highly homologous to that of human, rabbit, bovine, Rhesus monkey, and canine fVII. All protein domains of hfVII are strictly conserved in mfVII.
authors.affiliation: Department of Chemistry and Biochemistry, University of Notre Dame, Indiana 46556, USA.
authors.forename: E
authors.initials: E
authors.lastname: Idusogie
authors.forename: E
authors.initials: E
authors.lastname: Rosen
authors.forename: J P
authors.initials: JP
authors.lastname: Geng
authors.forename: P
authors.initials: P
authors.lastname: Carmeliet
authors.forename: D
authors.initials: D
authors.lastname: Collen
authors.forename: F J
authors.initials: FJ
authors.lastname: Castellino
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D003001:Cloning, Molecular; D003327:Coronary Disease / Q000235:genetics / Q000503:physiopathology*; D018076:DNA, Complementary / Q000235:genetics*; D004285:Dogs; D005167:Factor VII / Q000235:genetics*; D005815:Genetic Code; D006801:Humans; D008253:Macaca mulatta; D051379:Mice; D008969:Molecular Sequence Data; D017354:Point Mutation; D011817:Rabbits; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
chemical_list: D018076:DNA, Complementary; D005167:Factor VII