Application of capillary electrophoresis in the identification of phenotypes containing the beta-lactoglobulin C variant.
A method for the separation of the beta-LG variants A, B, and C was developed using free zone capillary electrophoresis. A separation buffer consisting of 50 mM 2-(N-morpholino)ethane sulfonic acid (pH 8.0) and polyoxyethylene (20)-sorbitan monolaurate was used for the separation. Milk samples from 424 Jersey cows were phenotyped using this method, and the gene frequencies for beta-LG A, B, and C were .41, .53, and .06, respectively. These frequencies were different from those found in studies of Jersey populations in other countries, where the beta-LG B allele had a significantly higher frequency, and the beta-LG A allele a significantly lower frequency, than those of this present study. The frequency of the beta-LG C allele was intermediate to those of other studies. The concentration of beta-LG in the milk was different among the beta-LG phenotypes and was, from greatest to least: AA, AB, AC, BB, and BC.
Paterson
G R
GR
Food Science Section, New Zealand Diary Research Institute, Palmerston North, New Zealand.
Otter
D E
DE
Hill
J P
JP
D000818:Animals; D002417:Cattle; D019075:Electrophoresis, Capillary; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D014644:Genetic Variation; D007774:Lactation; D007782:Lactoglobulins / Q000235:genetics / Q000302:isolation & purification*; D008892:Milk / Q000737:chemistry*; D010641:Phenotype
D007782:Lactoglobulins