title: Note: development of an external quality assurance scheme for the detection and enumeration of Pseudomonas aeruginosa from water and comparison of results using modified King's A broth and a commercial agar.
abstract: Two trials of the isolation and enumeration of a given strain of Pseudomonas aeruginosa from water are reported. In each trial participants received concentrated samples from two batches, one with low and one with high counts, to be diluted to 500 ml in sterile distilled or deionized water and examined for Ps. aeruginosa by membrane filtration. Membranes were incubated at 37 degrees C for 48 h on pads soaked in modified King's A broth (MKAB) and Unipath Pseudomonas Agar plus CFC supplement (PCFC). The first trial involved eight Public Health Laboratories (PHL) and the organizers provided media from single batches. The second trial, involving 50 PHL, examined the feasibility of a large scale external quality assessment (EQA) distribution. Participants were invited to use the same two media and their usual medium if different. Average counts were close to expected and the spread of results was comparable to that observed from the EQA scheme for indicator organisms. From the results of the two trials a better isolation of the strain of Ps. aeruginosa under consideration was noted with PCFC compared with MKAB.
authors.affiliation: Public Health Laboratory, General Hospital, Newcastle upon Tyne, UK.
authors.forename: B M
authors.initials: BM
authors.lastname: Place
authors.forename: H E
authors.initials: HE
authors.lastname: Tillett
authors.forename: N F
authors.initials: NF
authors.lastname: Lightfoot
mesh_terms: D003470:Culture Media; D011550:Pseudomonas aeruginosa / Q000302:isolation & purification*; D011786:Quality Control; D014871:Water Microbiology
chemical_list: D003470:Culture Media