Enhancing immunoelectrochemiluminescence (IECL) for sensitive bacterial detection.
Immunoelectrochemiluminescence (IECL) as an alternative method versus immunochemiluminescent and immunofluorescent methods can be used for versatile applications in biological agent detection. Although IECL offered high reproducibility and sensitive detection capability for soluble antigens and nucleic acids in aqueous phase, the IECL assays are not optimal and many factors which can affect the IECL performance still remain unclear. Further IECL kinetic studies, improvement of antibody biotinylation, magnetic particle selection and reducing non-specific binding have shown that the enhanced IECL sensitivities (signal to background noise ratios) can be potentially increased at least ten-fold compared to the sensitivities with general IECL assay procedures for bacterial detection.
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Calspan SRL Corporation, USAF-AL, Aberdeen Proving Ground, MD 21010, USA.
D000942:Antigens, Bacterial / Q000032:analysis*; D001408:Bacillus anthracis / Q000276:immunology; D001412:Bacillus subtilis / Q000276:immunology; D001426:Bacterial Proteins; D001666:Binding Sites, Antibody; D001710:Biotin; D004563:Electrochemistry; D004926:Escherichia coli / Q000276:immunology / Q000302:isolation & purification; D005434:Flow Cytometry; D007120:Immunochemistry / Q000379:methods*; D018189:Immunomagnetic Separation; D007700:Kinetics; D008163:Luminescent Measurements; D008856:Microscopy, Fluorescence; D012680:Sensitivity and Specificity; D019809:Streptavidin
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D001710:Biotin; D019809:Streptavidin