[Disruption of processing of alkaline phosphatase as a result of single amino acid changes affects the composition and metabolism of phospholipids from Escherichia coli, secreting mutant proteins].
Amino acid substitutions in the cleavage site of the E. coli alkaline phosphatase signal peptide Val for Ala(-1) or Pro for Arg(+1) result in the block of the enzyme processing. In cells secreting such mutant proteins the relative content and rate of turnover of anionic phospholipids (phosphatidylglycerol and cardiolipin) are increased. The rise of the transfer of the phosphoglycerol residue from phosphatidylglycerol to periplasmic membrane derived oligosaccharides or to the model substrate, arbutin performed by the activity of phosphoglycerol transferase I testifies to phosphatidylglycerol accumulation on the outer surface of the cytoplasmic membrane. The results suggest of phosphatidylglycerol interaction with the alkaline phosphatase precursor and their subsequent joint translocation through the cytoplasmic membrane of E. coli.
Kalinin
A E
AE
Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Moscow.
Karamyshev
A L
AL
Nesmeianova
M A
MA
D000469:Alkaline Phosphatase / Q000235:genetics / Q000378:metabolism*; D002308:Cardiolipins / Q000378:metabolism*; D004792:Enzyme Precursors / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D016297:Mutagenesis, Site-Directed; D010743:Phospholipids / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D021382:Protein Sorting Signals / Q000378:metabolism; D013347:Subcellular Fractions / Q000201:enzymology
D002308:Cardiolipins; D004792:Enzyme Precursors; D010743:Phospholipids; D021382:Protein Sorting Signals; D000469:Alkaline Phosphatase
Narushenie protsessinga shchelochnoĭ fosfatazy kak sledstvie edinichnykh zamen aminokislot, vliiaet na sostav i obmen fosfolipidov kletok Escherichia coli, sekretiruiushchikh mutantnye belki.