The Drosophila tamou gene, a component of the activating pathway of extramacrochaetae expression, encodes a protein homologous to mammalian cell-cell junction-associated protein ZO-1.
In Drosophila sensory organ development, the balance of activities between proneural genes and repressor genes defines a proneural cluster as a population of competent cells for neural development. In this study, we report the isolation and analysis of the tamou (tam) gene that encodes a cell-cell junction-associated protein, which is homologous to mammalian ZO-1, a member of the membrane-associated guanylate kinase homolog family. The tam mutation reduces the transcription of a repressor gene, extramacrochaetae, and causes enlargement of a proneural cluster where supernumerary precursor cells emerge, resulting in extra mechanosensory organs in the fly. These results suggest that the membrane-associated Tam protein is involved in the signaling pathway that activates emc expression.
Takahisa
M
M
Mitsubishi Kasei Institute of Life Sciences, Machida-shi, Tokyo 194, Japan.
Togashi
S
S
Suzuki
T
T
Kobayashi
M
M
Murayama
A
A
Kondo
K
K
Miyake
T
T
Ueda
R
R
D000595:Amino Acid Sequence; D000818:Animals; D030801:Animals, Genetically Modified; D001483:Base Sequence; D051792:Basic Helix-Loop-Helix Proteins; D002455:Cell Division / Q000235:genetics; D002874:Chromosome Mapping; D018076:DNA, Complementary / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D004330:Drosophila / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism; D029721:Drosophila Proteins; D017344:Genes, Insect; D007365:Intercellular Junctions / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D010641:Phenotype; D010750:Phosphoproteins / Q000235:genetics*; D012313:RNA / Q000235:genetics / Q000378:metabolism; D012097:Repressor Proteins; D012679:Sense Organs / Q000254:growth & development; D017386:Sequence Homology, Amino Acid; D062826:Zonula Occludens-1 Protein; D062827:Zonula Occludens-2 Protein
D051792:Basic Helix-Loop-Helix Proteins; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D008565:Membrane Proteins; D010750:Phosphoproteins; D012313:RNA; D012097:Repressor Proteins; D062826:Zonula Occludens-1 Protein; D062827:Zonula Occludens-2 Protein; C063577:emc protein, Drosophila