Imaging and biodistribution of 125I tyramine oligonucleotide in nude mice bearing human breast tumor. Preliminary report.
Although the range of applications for antisense oligonucleotides is vast, current research concentrates mainly on virology and oncology. We have conducted in vivo and in vitro investigations of radiolabelling and biodistribution of a 22-mer phosphodiester oligonucleotide injected in athymic mice bearing xenograft of human mammary tumor (coculture: MCF7 and fibroblasts strain AF-11). Tumor/healthy tissue ratio of the 22-mer phosphodiester oligonucleotide fixation is high during the 24 hours after injection instead of fast elimination.
Cammilleri
S
S
Nuclear Medicine Department, CHU Timone, Marseille, France.
Perdereau
B
B
Brixy
F
F
Pierga
J Y
JY
Benyahia
B
B
Chypre
C
C
Magdelenat
H
H
D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D007457:Iodine Radioisotopes / Q000493:pharmacokinetics; D008325:Mammary Neoplasms, Experimental / Q000000981:diagnostic imaging*; D051379:Mice; D008819:Mice, Nude; D016376:Oligonucleotides, Antisense / Q000493:pharmacokinetics*; D014018:Tissue Distribution; D014055:Tomography, Emission-Computed; D014439:Tyramine
D007457:Iodine Radioisotopes; D016376:Oligonucleotides, Antisense; D014439:Tyramine