Spermidine regulation of protein synthesis at the level of initiation complex formation of Met-tRNAi, mRNA and ribosomes.
Spermidine regulation of protein synthesis (stimulation at low concentrations and inhibition at high concentrations) was studied using a mRNA with a GC-rich 5'-untranslated region. It was found that the initiation complex formation of mRNA, Met-tRNAi, and 40 S ribosomal subunits was regulated by spermidine. The inhibition of initiation complex formation at high spermidine concentrations was greater with 80 S ribosomes than with 40S ribosomal subunits. This was partially explained by the spermidine inhibition of initiation factor-dependent RNA helicase activity.
Shimogori
T
T
Faculty of Pharmaceutical Sciences, Chiba University, Japan.
Kashiwagi
K
K
Igarashi
K
K
D000199:Actins / Q000235:genetics; D012437:Adenosylmethionine Decarboxylase / Q000235:genetics; D000818:Animals; D001482:Base Composition; D001483:Base Sequence; D002474:Cell-Free System; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D016366:Open Reading Frames; D009955:Ornithine Decarboxylase / Q000235:genetics / Q000378:metabolism; D010442:Peptide Chain Initiation, Translational / Q000187:drug effects*; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism*; D012358:RNA, Transfer, Met / Q000378:metabolism*; D011817:Rabbits; D012156:Reticulocytes / Q000378:metabolism; D012270:Ribosomes / Q000187:drug effects / Q000378:metabolism*; D013095:Spermidine / Q000494:pharmacology*; D013937:Thymidine Kinase / Q000235:genetics
D000199:Actins; D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger; D012358:RNA, Transfer, Met; D013937:Thymidine Kinase; D009955:Ornithine Decarboxylase; D012437:Adenosylmethionine Decarboxylase; D013095:Spermidine