title: Carbohydrate moieties of three radish peroxidases.
abstract: The carbohydrate moieties of two anionic peroxidases, termed A1 and A2, and one cationic peroxidase, named C3, from Korean radish (Raphanus sativus) were studied. For profiling of N-glycans, each peroxidase was treated with peptidyl N-glycosidase F and hydrazine. These peroxidases were more susceptible to hydrazine than to peptidyl N-glycosidase F. When these three peroxidases were subjected to trifluoroacetic acid treatment, mannose, fucose and N-acetylglucosamine were released. Two major N-glycans of peroxidase C3 were isolated and treated with several glycohydrolases. Analysis of digested products of the two major N-glycans on polyacrylamide gel suggested that core-fucosylated trimannosylchitobiose may contain a different linkage from the typical alpha-1,6 of native N-linked oligosaccharide.
authors.affiliation: Department of Biochemistry, College of Science and Bioproducts Research Center, Yonsei University, Seoul, Korea.
authors.forename: S H
authors.initials: SH
authors.lastname: Kim
authors.forename: S S
authors.initials: SS
authors.lastname: Kim
mesh_terms: D000117:Acetylglucosamine / Q000032:analysis; D002240:Carbohydrate Sequence; D005643:Fucose / Q000032:analysis; D006023:Glycoproteins / Q000737:chemistry*; D006026:Glycoside Hydrolases; D008358:Mannose / Q000032:analysis; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry; D010544:Peroxidases / Q000737:chemistry*; D014675:Vegetables / Q000201:enzymology*
chemical_list: D006023:Glycoproteins; D009844:Oligosaccharides; D005643:Fucose; D010544:Peroxidases; D006026:Glycoside Hydrolases; D008358:Mannose; D000117:Acetylglucosamine