title: Characterization of small intestinal submucosa regenerated canine detrusor: assessment of reinnervation, in vitro compliance and contractility.
abstract: OBJECTIVE
  We characterized small intestinal submucosa regenerated canine bladder.
  
  
  METHODS
  We subjected 15-month small intestinal submucosa regenerated canine bladder strips to in vitro muscle bath compliance, contractility testing and immunohistochemical staining.
  
  
  RESULTS
  Compliance studies demonstrated no significant difference between small intestinal submucosa regenerated and control bladders, which were 30-fold more compliant than native small intestinal submucosal graft material. Contractility studies demonstrated contractile responses and innervation similar to those of normal canine bladder. Afferent nerves were demonstrated through immunohistochemical techniques.
  
  
  CONCLUSIONS
  These characteristics further support the regenerative capacity of small intestinal submucosa and its potential use as a bladder augmentation material.
authors.affiliation: Department of Urology, Riley Children's Hospital, Indiana University School of Medicine, Lafayette, USA.
authors.forename: B P
authors.initials: BP
authors.lastname: Kropp
authors.forename: B D
authors.initials: BD
authors.lastname: Sawyer
authors.forename: H E
authors.initials: HE
authors.lastname: Shannon
authors.forename: M K
authors.initials: MK
authors.lastname: Rippy
authors.forename: S F
authors.initials: SF
authors.lastname: Badylak
authors.forename: M C
authors.initials: MC
authors.lastname: Adams
authors.forename: M A
authors.initials: MA
authors.lastname: Keating
authors.forename: R C
authors.initials: RC
authors.lastname: Rink
authors.forename: K B
authors.initials: KB
authors.lastname: Thor
mesh_terms: D000818:Animals; D004285:Dogs; D066298:In Vitro Techniques; D007413:Intestinal Mucosa / Q000637:transplantation*; D007421:Intestine, Small / Q000637:transplantation*; D009119:Muscle Contraction / Q000187:drug effects / Q000502:physiology*; D009130:Muscle, Smooth / Q000187:drug effects / Q000502:physiology*; D012038:Regeneration; D001743:Urinary Bladder / Q000294:innervation* / Q000502:physiology* / Q000601:surgery