Refined carbohydrate enhancement of aberrant crypt foci (ACF) in rat colon induced by the food-borne carcinogen 2-amino-3-methyl-imidazo[4,5-f]quinoline (IQ).
The aberrant crypt foci (ACF) bioassay has been used extensively to study the early effects of different dietary components on the colonic mucosa of laboratory rodents. ACF are proposed to represent preneoplastic lesions of colon cancer. Compared to the normally used initiators 1,2-dimethylhydrazine dihydrochloride (DMH) and azoxymethane (AOM), the use of a diet-related colon cancer initiator, such as the heterocyclic amine 2-amino-3-methyl-imidazo[4,5-f]quinoline (IQ) formed during meat cooking, would probably give a more relevant insight into diet-related colon carcinogenesis. In the present study it is shown that a feeding regimen with continuous low IQ doses (0.03% in the diet) throughout a study period of 10 weeks has a significant effect on the induction of ACF in the colon of male F344 rats. In addition, the study illustrates that the incidence of the IQ-induced ACF can be modulated by the amount of refined carbohydrates in the diet. Rats given a high sucrose/dextrin diet showed a significantly higher number of ACF compared to rats given a diet high in starches. The effect on tumor outcome will await the termination of a ongoing parallel study.
Kristiansen
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Institute of Toxicology, National Food Agency, Mørkhøj Bygade, Søborg, Denmark. ek@1st.min.dk
Meyer
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Thorup
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D000284:Administration, Oral; D000818:Animals; D015197:Carcinogenicity Tests; D002273:Carcinogens / Q000008:administration & dosage; D003106:Colon / Q000473:pathology; D003110:Colonic Neoplasms / Q000473:pathology*; D004040:Dietary Carbohydrates / Q000494:pharmacology*; D005526:Food, Formulated; D008297:Male; D011230:Precancerous Conditions / Q000139:chemically induced*; D011804:Quinolines / Q000008:administration & dosage; D051381:Rats; D011916:Rats, Inbred F344
D002273:Carcinogens; D004040:Dietary Carbohydrates; D011804:Quinolines; C029216:2-amino-3-methylimidazo(4,5-f)quinoline