title: Identification and characterization of the Drosophila histone H4 replacement gene.
abstract: Replacement variant genes for different histones have been described in most higher eukaryotes. However, so far no such gene has been found for histone H4. We have isolated from both Drosophila melanogaster and D. hydei a novel histone H4 encoding gene, H4r, which displays all the properties of a histone replacement variant gene: it contains introns, generates polyadenylated mRNA, represents the predominant H4 transcript in non-dividing tissues and is present in the genome as a single copy. The encoded polypeptide is identical to the Drosophila cell-cycle regulated histone H4. The fact that it is a single copy gene makes it prone to genetic analysis and it might be a useful tool for studying nucleosome structure and function.
authors.affiliation: Department of Molecular and Developmental Genetics, Faculty of Sciences, Catholic University of Nijmegen, The Netherlands.
authors.forename: A
authors.initials: A
authors.lastname: Akhmanova
authors.forename: K
authors.initials: K
authors.lastname: Miedema
authors.forename: W
authors.initials: W
authors.lastname: Hennig
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005075:Biological Evolution; D015152:Blotting, Northern; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004330:Drosophila / Q000235:genetics* / Q000378:metabolism; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D006657:Histones / Q000235:genetics*; D008969:Molecular Sequence Data
chemical_list: D018076:DNA, Complementary; D029721:Drosophila Proteins; C100492:His4r protein, Drosophila; D006657:Histones