Collagen types I and III localization by in situ hybridization and immunohistochemistry in the partially obstructed young rabbit bladder.
OBJECTIVE
The objective of the study was to investigate the alteration of collagen expression patterns at the transcription and translation levels during partial outlet obstruction and to compare changes in collagen types I and III gene expression, and protein deposition during early and subacute phases of obstruction.


METHODS
Following 1, 2, 7, 14 and 21 days of partial bladder obstruction in 2-week-old rabbits bladder tissues were evaluated for collagen types I and III messenger ribonucleic acid localization by in situ hybridization and for protein localization by immunohistochemical study.


RESULTS
There is up regulation of collagen types I and III gene expression after obstruction. Up regulation of collagen gene expression immediately after obstruction is within the lamina propria and the message moves into the muscular layer as obstruction progresses. Protein deposition for both collagen types is analogous to that of gene expression.


CONCLUSIONS
Up regulation of collagen types I and III gene expression, and increased protein deposition after partial obstruction demonstrate that the alteration in collagen synthesis is at least partially transcription regulated. This alteration starts from superficial layers of the bladder wall and becomes deeper as the pathological process progresses.
Tekgul
S
S
Division of Pediatric Urology, University of Washington, Seattle, USA.
Yoshino
K
K
Bagli
D
D
Carr
M C
MC
Mitchell
M E
ME
Yao
L Y
LY
D000367:Age Factors; D000818:Animals; D003094:Collagen / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D005786:Gene Expression Regulation; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D009929:Organ Size; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000032:analysis; D011817:Rabbits; D014158:Transcription, Genetic; D001743:Urinary Bladder / Q000473:pathology; D001748:Urinary Bladder Neck Obstruction / Q000378:metabolism*
D012333:RNA, Messenger; D003094:Collagen