Influence of salbutamol and isoproterenol on the production of TNF and reactive oxygen species by bovine alveolar macrophages and calcitriol differentiated HL-60 cells.
The influence of the beta-adrenergic agonists Salbutamol and Isoproterenol on the release of reactive oxygen species and Tumor Necrosis Factor (TNF) was tested with bovine alveolar macrophages and HL-60 cells differentiated to macrophages by calcitriol. The production of reactive oxygen species was analyzed by a microplate assay using dichlorofluorescein-diacetate. It could be shown that this method almost exclusively measures superoxide anions. TNF was determined by a bioassay with WEHI cells. The superoxide anion production was stimulated by Zymosan, the TNF release by LPS. By incubation with 5 x 10(-6) and 5 x 10(-7) M Salbutamol or 5 x 10(-7) and 5 x 10(-8) M Isoproterenol prior to the stimulation, the production of superoxide anions as well as of TNF was inhibited to a significant degree. The inhibitory effects of the adrenergic agonists were completely or at least partially inhibited by the respective antagonists, ICI 118.551 and Propranolol, respectively.
Gu
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Forschungszentrum Karlsruhe, Technik und Umwelt, Institut für Toxikologie, Germany.
Seidel
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D000420:Albuterol / Q000494:pharmacology*; D000818:Animals; D002117:Calcitriol / Q000494:pharmacology*; D002417:Cattle; D002454:Cell Differentiation / Q000187:drug effects; D018922:HL-60 Cells; D006801:Humans; D007545:Isoproterenol / Q000494:pharmacology*; D016676:Macrophages, Alveolar / Q000187:drug effects* / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*
D017382:Reactive Oxygen Species; D014409:Tumor Necrosis Factor-alpha; D002117:Calcitriol; D007545:Isoproterenol; D000420:Albuterol