Complementary effects of paeoniflorin and glycyrrhizin on intracellular Ca2+ mobilization in the nerve-stimulated skeletal muscle of mice.
Effects of paeoniflorin (PF) and glycyrrhizin (GLR), contained in paeony and licorice roots, respectively, on contractile and non-contractile Ca2+ mobilization were examined by measuring the Ca(2+)-aequorin luminescence (Ca2+ transients) of the nerve-stimulated skeletal muscle of mice in the presence of neostigmine (0.3 microM). PF (0.1-1 mM) prolonged the duration of non-contractile Ca2+ transients, which may induce the desensitization of nicotinic acetylcholine receptor, but did not affect contractile Ca2+ transients. GLR (0.3-1 mM) depressed contractile Ca2+ transients without affecting non-contractile transients. These results suggest that PF and GLR may have complementary effects on intracellular Ca2+ mobilization to block the neuromuscular transmission.
Dezaki
K
K
Department of Chemical Pharmacology, Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University, Japan.
Kimura
I
I
Miyahara
K
K
Kimura
M
M
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D001565:Benzoates; D001952:Bridged-Ring Compounds; D002118:Calcium / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D005960:Glucosides / Q000494:pharmacology*; D006034:Glycyrrhetinic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D019695:Glycyrrhizic Acid; D051379:Mice; D008815:Mice, Inbred Strains; D039821:Monoterpenes; D018482:Muscle, Skeletal / Q000187:drug effects*; D006027:Glycosides
D000894:Anti-Inflammatory Agents, Non-Steroidal; D001565:Benzoates; D001952:Bridged-Ring Compounds; D002118:Calcium; D005960:Glucosides; D006034:Glycyrrhetinic Acid; D019695:Glycyrrhizic Acid; D039821:Monoterpenes; C015423:peoniflorin; D006027:Glycosides