title: The arsenical resistance operon of IncN plasmid R46.
abstract: The arsenical resistance operon of the IncN plasmid R46 consists of 4696 bp and starts with predicted transcriptional control and initiation signals, followed by five genes, arsD, arsA, and arsC. The corresponding Escherichia coli chromosomal ars operon and two staphylococcal ars operons lack arsA and arsD genes. The R46 system contains only the second known versions of arsA and arsD, after those of plasmid R773. Western blot analysis identified the R46 proteins using antibodies against R773 ArsA, ArsD and ArsR.
authors.affiliation: Biotechnologies Department, Idaho National Engineering Laboratory, Idaho Falls 83415-2203, USA.
authors.forename: D F
authors.initials: DF
authors.lastname: Bruhn
authors.forename: J
authors.initials: J
authors.lastname: Li
authors.forename: S
authors.initials: S
authors.lastname: Silver
authors.forename: F
authors.initials: F
authors.lastname: Roberto
authors.forename: B P
authors.initials: BP
authors.lastname: Rosen
mesh_terms: D000251:Adenosine Triphosphatases / Q000235:genetics*; D001151:Arsenic / Q000494:pharmacology*; D053501:Arsenite Transporting ATPases; D001426:Bacterial Proteins; D001483:Base Sequence; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics*; D029968:Escherichia coli Proteins; D005798:Genes, Bacterial / Q000235:genetics; D016623:Ion Pumps; D008565:Membrane Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes; D009876:Operon / Q000235:genetics; D010957:Plasmids / Q000235:genetics; D017422:Sequence Analysis, DNA; D015534:Trans-Activators / Q000235:genetics
chemical_list: C489527:ArsR protein, E coli; D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D016623:Ion Pumps; D008565:Membrane Proteins; D009097:Multienzyme Complexes; D015534:Trans-Activators; D000251:Adenosine Triphosphatases; D053501:Arsenite Transporting ATPases; D001151:Arsenic