Molecular mechanisms in the disassembly and reassembly of the mammalian Golgi apparatus during M-phase.
The mitotic disassembly and reassembly of the mammalian Golgi apparatus is an ideal system to study the molecular mechanisms involved in biogenesis and maintenance of membranous organelles. As cells enter M-phase, Golgi stacks are converted into Golgi clusters of small membrane fragments, which are dispersed throughout the cytoplasmic space during metaphase. Disassembly is dependent on the action of cdc2-kinase and at least two distinct pathways contribute to the fragmentation: one involves the budding of COP I-coated vesicles from Golgi cisternae, the other is a less well characterised COP I-independent pathway. During telophase, the Golgi fragments reassemble and fuse into a fully functional Golgi stack, using at least two distinct ATPase-mediated fusion pathways.
Misteli
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Cold Spring Harbor Laboratory, NY 11724, USA. misteli@cshl.org
D000818:Animals; D006056:Golgi Apparatus / Q000378:metabolism / Q000502:physiology*; D008322:Mammals; D008938:Mitosis / Q000502:physiology*