title: Separation of the transcriptional coactivator and antirepression functions of transcription factor IIA.
abstract: Human transcription factor IIA (TFIIA) is composed of three subunits (alpha, beta, and gamma). TFIIA interacts with the TATA-box binding protein and can overcome repression of transcription. TFIIA was found to be necessary for VP16-mediated transcriptional activation through a coactivator function. We have separated the coactivator and antirepression activities of TFIIA. A TFIIA lacking the alpha subunit was isolated from HeLa cells. This "mini-TFIIA" interacts with the TATA-box binding protein and can overcome repression of transcription, but it is defective in transcriptional coactivator function.
authors.affiliation: Howard Hughes Medical Institute, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, Piscataway, 08854-5635, USA.
authors.forename: D
authors.initials: D
authors.lastname: MA
authors.forename: I
authors.initials: I
authors.lastname: Olave
authors.forename: A
authors.initials: A
authors.lastname: Merino
authors.forename: D
authors.initials: D
authors.lastname: Reinberg
mesh_terms: D004268:DNA-Binding Proteins / Q000378:metabolism; D005656:Fungal Proteins / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D012097:Repressor Proteins / Q000378:metabolism*; D016385:TATA Box; D035181:TATA-Box Binding Protein; D015534:Trans-Activators / Q000378:metabolism*; D035561:Transcription Factor TFIIA; D014157:Transcription Factors / Q000302:isolation & purification / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D004268:DNA-Binding Proteins; D005656:Fungal Proteins; C070633:Gal-VP16; D012097:Repressor Proteins; D035181:TATA-Box Binding Protein; D015534:Trans-Activators; D035561:Transcription Factor TFIIA; D014157:Transcription Factors