title: A linkage map of sweet cherry based on RAPD analysis of a microspore-derived callus culture population.
abstract: A partial linkage map was constructed for the sweet cherry (Prunus avium L.) cultivar Emperor Francis from a population of 56 microspore-derived callus culture individuals. The callus cultures were genotyped for two allozymes and 90 random amplified polymorphic DNA (RAPD) markers using 79 random decanucleotide DNA primers and the polymerase chain reaction (PCR). Eighty-nine markers mapped to 10 linkage groups totaling 503.3 cM. DNA blot and hybridization analysis using five cloned RAPDs as probes demonstrated that one of the decanucleotide primers amplified a region of the Emperor Francis genome containing a unique sequence, whereas the other four decanucleotide primers amplified regions of the Emperor Francis genome containing repeated sequences. The five cloned RAPD probes also recognized putative homologous regions in ground cherry, P.fruticosa Pall., and sour cherry, P. cerasus L., a naturally occurring allopolyploid between P.fruticosa and P.avium.
authors.affiliation: Department of Crop Science, Michigan State University, East Lansing 48824, USA.
authors.forename: E J
authors.initials: EJ
authors.lastname: Stockinger
authors.forename: C A
authors.initials: CA
authors.lastname: Mulinix
authors.forename: C M
authors.initials: CM
authors.lastname: Long
authors.forename: T S
authors.initials: TS
authors.lastname: Brettin
authors.forename: A F
authors.initials: AF
authors.lastname: Iezzoni
mesh_terms: D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D046508:Culture Techniques; D018744:DNA, Plant; D005638:Fruit / Q000235:genetics*; D008040:Genetic Linkage; D005819:Genetic Markers; D018745:Genome, Plant; D008969:Molecular Sequence Data; D019105:Random Amplified Polymorphic DNA Technique
chemical_list: D018744:DNA, Plant; D005819:Genetic Markers