title: In vivo studies on genotoxicity and cogenotoxicity of ingested UICC anthophyllite asbestos.
abstract: Early cytogenetic action of oral exposure to UICC anthophyllite, an amphibole type of asbestos, was studied in Fischer-344 rats. The animals were gavaged with a suspension of untreated fibres (50 mg/kg) and fibres which had been allowed to adsorb benzo[alpha]pyrene molecules from aqueous solutions of 0.25-2.5 micrograms/ml. HPLC measurements indicated effective adsorption of the benzo[alpha]pyrene molecules on the fibres. The authors consider this system a suitable model for the drinking of water containing asbestos fibres and organic micropollutants. The formation of micronuclei and sister chromatid exchanges was studied in bone marrow samples taken from animals 24 h after oral administration of suspensions. Whereas anthophyllite fibres failed to induce cytogenetic alterations, fibres pretreated with the polycyclic aromatic solutions caused dose-dependent increase in the sister chromatid exchange frequencies. The observed cytogenetic impact can be explained by a local action of carcinogen molecules accumulated and subsequently transported. The results support the hypothesis that epidemiological evidence of carcinogenicity of asbestos in potable water may rather be explained by cogenotoxic action of the asbestos fibres and biologically active organic micropollutants adsorbed on their surface.
authors.affiliation: Department of Hygiene and Epidemiology, University Medical School of Debrecen, Hungary.
authors.forename: C
authors.initials: C
authors.lastname: Varga
authors.forename: G
authors.initials: G
authors.lastname: Horváth
authors.forename: V
authors.initials: V
authors.lastname: Timbrell
mesh_terms: D000284:Administration, Oral; D000818:Animals; D017636:Asbestos, Amphibole / Q000008:administration & dosage / Q000737:chemistry / Q000633:toxicity*; D001564:Benzo(a)pyrene / Q000737:chemistry; D001853:Bone Marrow / Q000187:drug effects*; D001854:Bone Marrow Cells; D002273:Carcinogens / Q000008:administration & dosage / Q000633:toxicity*; D002851:Chromatography, High Pressure Liquid; D003582:Cytogenetics / Q000379:methods; D004912:Erythrocytes / Q000187:drug effects; D005260:Female; D006410:Hematopoiesis / Q000187:drug effects; D008938:Mitosis / Q000187:drug effects; D009152:Mutagenicity Tests; D051381:Rats; D011916:Rats, Inbred F344; D012854:Sister Chromatid Exchange / Q000187:drug effects
chemical_list: D017636:Asbestos, Amphibole; D002273:Carcinogens; D001564:Benzo(a)pyrene