One-step purification of recombinant proteins with the 6xHis tag and Ni-NTA resin.
The 6xHis/Ni-NTA system allows rapid and efficient affinity purification of recombinant proteins from virtually any expression system. Protocols and tips for purification under both native and denaturing conditions are provided, as well as a rapid spin procedure for protein minipreps.
Crowe
J
J
QIAGEN Inc., Chatsworth, CA 91311, USA.
Masone
B S
BS
Ribbe
J
J
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000096:biosynthesis; D002614:Chelating Agents; D002846:Chromatography, Affinity; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D005822:Genetic Vectors / Q000737:chemistry; D006639:Histidine / Q000737:chemistry*; D008969:Molecular Sequence Data; D009571:Nitrilotriacetic Acid / Q000031:analogs & derivatives* / Q000737:chemistry; D009942:Organometallic Compounds / Q000737:chemistry*; D010957:Plasmids / Q000235:genetics; D011489:Protein Denaturation; D011994:Recombinant Proteins / Q000302:isolation & purification*; D011995:Recombination, Genetic
D001426:Bacterial Proteins; D002614:Chelating Agents; D009942:Organometallic Compounds; D011994:Recombinant Proteins; C088321:nickel nitrilotriacetic acid; D006639:Histidine; D009571:Nitrilotriacetic Acid