title: The sheep Ig variable region repertoire consists of a single VH family.
abstract: Nine germ-line Ig heavy chain variable (VH) segments (including three pseudogenes) were isolated from a genomic DNA library, and the other six were obtained by PCR, using 5'and 3' primers deduced from the first three. They appear to belong to a homogeneous VH gene family, with >80% sequence identity. This sheep VH gene family is related to the human VH4 family and to the murine VH1 subgroup (clan II). Southern blot analysis shows a maximum of 10 positive restriction fragments; therefore, the nine VH genes isolated probably constitute the major part of the repertoire. Thirty-one expressed mu variable regions (and one gamma 1 variable region) were obtained from adult spleen by either cDNA cloning or anchored reverse transcriptase-PCR; they are >80% similar to each other (in their leader to framework 3 regions) and to the germ-line sequences as well. The sheep VH repertoire thus seems to derive from a small (approximately 10 members) germ-line gene family, and its diversification must rely chiefly on junctional (D and/or N regions) diversity and somatic hypermutations.
authors.affiliation: Institute for Molecular Biology and Genetic Engineering, CNRS URA 1172, Poitiers, France.
authors.forename: V
authors.initials: V
authors.lastname: Dufour
authors.forename: S
authors.initials: S
authors.lastname: Malinge
authors.forename: F
authors.initials: F
authors.lastname: Nau
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D001483:Base Sequence; D015139:Blotting, Southern; D004247:DNA / Q000302:isolation & purification; D005786:Gene Expression Regulation; D015326:Gene Rearrangement, B-Lymphocyte, Heavy Chain / Q000276:immunology; D005803:Genes, Immunoglobulin / Q000276:immunology*; D007143:Immunoglobulin Heavy Chains / Q000235:genetics* / Q000302:isolation & purification; D007135:Immunoglobulin Variable Region / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000276:immunology*; D011544:Pseudogenes / Q000276:immunology; D012756:Sheep / Q000276:immunology*; D013154:Spleen / Q000378:metabolism
chemical_list: D007143:Immunoglobulin Heavy Chains; D007135:Immunoglobulin Variable Region; D004247:DNA