[The use of a new strain of Vibrio cholerae O139 as a producer of an enteric chemical vaccine].
Conditions for the submerged cultivation of a strain of V. cholerae O139, were worked out. These conditions ensured a high yield of biomass, soluble O-antigen and exoenzymes (proteinase, phospholipase A) into the culture medium, which exceeded their production by strains of serovar O1, respectively, 2, 3, 4 and 8 times. The preparation, isolated from the culture fluid and lyophilized, contained up to 50% of O-antigen and exoenzymes. In experiments on white mice the preparation exhibited low toxicity (LD50 was equal, on the average, to 1.2 mg) and immunogenicity (ED50 was equal to 3-5 micrograms) with respect to V. cholerae O139, which corresponded to the protective potency of commercial vaccine against V. cholerae O1 infection.
Dzhaparidze
M N
MN
Naumov
A V
AV
Gromova
O V
OV
Adamov
A K
AK
Eliseev
Iu Iu
IuIu
Kosmoenko
O M
OM
Kuz'michenko
I A
IA
Kopyrzov
V N
VN
Zavorotnykh
V I
VI
Zakharova
T L
TL
Chekhovskaia
G V
GV
D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology / Q000302:isolation & purification / Q000633:toxicity; D001428:Bacterial Vaccines / Q000096:biosynthesis* / Q000276:immunology / Q000302:isolation & purification / Q000633:toxicity; D003470:Culture Media; D000939:Epitopes / Q000276:immunology / Q000302:isolation & purification / Q000633:toxicity; D007114:Immunization; D007928:Lethal Dose 50; D051379:Mice; D011817:Rabbits; D014614:Vaccines, Synthetic / Q000096:biosynthesis* / Q000276:immunology / Q000302:isolation & purification / Q000633:toxicity; D014734:Vibrio cholerae / Q000254:growth & development / Q000276:immunology*
D000942:Antigens, Bacterial; D001428:Bacterial Vaccines; D003470:Culture Media; D000939:Epitopes; D014614:Vaccines, Synthetic
Ispol'zovanie novogo shtamma Vibrio cholerae O139 v kachestve produtsenta énteral'noĭ khimicheskoĭ vaktsiny.