title: Phosphorylation of myosin light chain at distinct sites and its association with the cytoskeleton during enteropathogenic Escherichia coli infection.
abstract: Myosin light chain, the most prominent host cell phosphoprotein during adhesion of enteropathogenic Escherichia coli to cultured HEp-2 cells, was shown to be distributed between cytosolic and cytoskeletal cell fractions; its association with the cytoskeletal fraction increased with time of enteropathogenic E. coli incubation. Phosphopeptide mapping indicated cytosolic and cytoskeletal myosin light chain phosphorylation at different sites by protein kinase C and myosin light chain kinase.
authors.affiliation: Laboratory of Protein Structure, National Institute for Medical Structure, London, United Kingdom.
authors.forename: H A
authors.initials: HA
authors.lastname: Manjarrez-Hernandez
authors.forename: T J
authors.initials: TJ
authors.lastname: Baldwin
authors.forename: P H
authors.initials: PH
authors.lastname: Williams
authors.forename: R
authors.initials: R
authors.lastname: Haigh
authors.forename: S
authors.initials: S
authors.lastname: Knutton
authors.forename: A
authors.initials: A
authors.lastname: Aitken
mesh_terms: D002478:Cells, Cultured; D003599:Cytoskeleton / Q000378:metabolism*; D003967:Diarrhea / Q000209:etiology*; D004926:Escherichia coli / Q000472:pathogenicity*; D006801:Humans; D018994:Myosin Light Chains / Q000378:metabolism*; D010766:Phosphorylation
chemical_list: D018994:Myosin Light Chains