Comparison of capillary electrophoretic and liquid chromatographic determination of hypoxanthine and xanthine for the diagnosis of xanthinuria.
A capillary electrophoretic (CE) method for the determination of hypoxanthine and xanthine in urine was developed to diagnose xanthinuria. The linearity was excellent up to 200 mumol l-1 for the two compounds and the limit of quantitation was 2 mumol l-1. A comparison o the results obtained using CE was made with those obtained by the high-performance liquid chromatographic (HPLC) technique described previously. With regard to specificity, sensitivity and reproducibility, the results are similar but CE is more rapid than HPLC.
Bory
C
C
Laboratoire d'Etudes des Maladies Métaboliques, Hôpital Debrousse, Lyon, France.
Chantin
C
C
Boulieu
R
R
D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000706:statistics & numerical data; D019075:Electrophoresis, Capillary / Q000379:methods* / Q000706:statistics & numerical data; D006801:Humans; D019271:Hypoxanthine; D007042:Hypoxanthines / Q000652:urine*; D011686:Purine-Pyrimidine Metabolism, Inborn Errors / Q000175:diagnosis* / Q000652:urine; D019820:Xanthine; D014970:Xanthines / Q000652:urine*
D007042:Hypoxanthines; D014970:Xanthines; D019820:Xanthine; D019271:Hypoxanthine