Thymidylate synthase in advanced gastrointestinal and breast cancers.
Knowledge of population thymidylate synthase (TS) levels in malignant tumors and normal tissues is essential for the use of TS as a predictor for 5-fluorouracil treatment. Tumor tissue TS levels in fresh frozen surgical biopsies from 136 patients with gastrointestinal or breast cancer, not previously subjected to chemotherapy, were analysed by [3H]FdUMP radioligand binding assay. TS levels were 2.4 +/- 0.31 pmol/g in liver metastases of colorectal cancer (n = 87), 4.2 +/- 1.0 pmol/g in primary colorectal cancer (n = 13), 2.7 +/- 0.93 pmol/g in gastric cancer (n = 13), 3.1 +/- 1.7 pmol/g in pancreatic cancer (n = 10), 3.4 +/- 1.4 pmol/g in breast cancer (n = 13) and 0.58 +/- 0.075 pmol/g in normal liver parenchyma (n = 24). TS levels were significantly higher in malignant tumor tissues compared to normal liver parenchyma.
Larsson
P A
PA
Department of Surgery, Ostra Sjukhuset, Göteborg, Sweden.
Carlsson
G
G
Gustavsson
B
B
Spears
C P
CP
D000964:Antimetabolites, Antineoplastic / Q000627:therapeutic use; D014408:Biomarkers, Tumor / Q000032:analysis; D001706:Biopsy; D001943:Breast Neoplasms / Q000188:drug therapy / Q000201:enzymology* / Q000473:pathology; D003110:Colonic Neoplasms / Q000201:enzymology / Q000473:pathology; D005260:Female; D005472:Fluorouracil / Q000627:therapeutic use; D005544:Forecasting; D005629:Frozen Sections; D005770:Gastrointestinal Neoplasms / Q000188:drug therapy / Q000201:enzymology* / Q000473:pathology; D006801:Humans; D008099:Liver / Q000201:enzymology / Q000473:pathology; D008113:Liver Neoplasms / Q000201:enzymology / Q000556:secondary; D010190:Pancreatic Neoplasms / Q000201:enzymology / Q000473:pathology; D012004:Rectal Neoplasms / Q000201:enzymology / Q000473:pathology; D013274:Stomach Neoplasms / Q000201:enzymology / Q000473:pathology; D013940:Thymidylate Synthase / Q000032:analysis*; D014316:Tritium
D000964:Antimetabolites, Antineoplastic; D014408:Biomarkers, Tumor; D014316:Tritium; D013940:Thymidylate Synthase; D005472:Fluorouracil