p53 and MDM2 expression in oral squamous cell carcinoma.
The p53 tumor suppressor gene is the most commonly mutated gene in human cancer and is a frequent abnormality in oral squamous cell carcinoma and its precancerous lesions. MDM2 (murine double minute-2), a new proto-oncogene, may be associated with p53 gene products and may negatively affect the transcriptional activating function of p53. The purpose of this study was to investigate the incidence of MDM2 and its relationship to the expression of p53 in oral squamous cell carcinoma and precancerous lesions. Overexpression of p53 and MDM2 proteins was detected in 52 and 40% of oral squamous cell carcinomas, respectively. p53 gene mutation, absent in normal oral epithelium was observed in 31% of the carcinoma cases. Our finding suggested that MDM2 protein may be an alternative mechanism causing p53 protein dysfunction in oral squamous cell carcinoma.
Matsumura
T
T
Department of Oral and Maxillofacial Surgery II, Okayama University Dental School, Japan.
Yoshihama
Y
Y
Kimura
T
T
Shintani
S
S
Alcalde
R E
RE
D001483:Base Sequence; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000473:pathology*; D017931:DNA Primers; D015870:Gene Expression; D016158:Genes, p53; D006801:Humans; D007150:Immunohistochemistry; D008969:Molecular Sequence Data; D009062:Mouth Neoplasms / Q000235:genetics / Q000473:pathology*; D009154:Mutation; D009363:Neoplasm Proteins / Q000032:analysis / Q000096:biosynthesis; D009687:Nuclear Proteins; D016376:Oligonucleotides, Antisense; D016133:Polymerase Chain Reaction; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins / Q000032:analysis / Q000096:biosynthesis*; D051736:Proto-Oncogene Proteins c-mdm2; D011519:Proto-Oncogenes; D012189:Retrospective Studies; D016159:Tumor Suppressor Protein p53 / Q000032:analysis / Q000096:biosynthesis*
D017931:DNA Primers; C000715470:MAS1 protein, human; D009363:Neoplasm Proteins; D009687:Nuclear Proteins; D016376:Oligonucleotides, Antisense; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins; D016159:Tumor Suppressor Protein p53; C497206:MDM2 protein, human; D051736:Proto-Oncogene Proteins c-mdm2