[Spermatozoa antigen SAA-1 and co-precipitating molecules: regulation of human acrosome reaction].
OBJECTIVE
Examination of the regulation of the human sperm acrosome reaction as an example of ligand-induced exocytosis.


METHODS
Development of monoclonal antibodies (mab) against immunaffinity-purified sperm antigen SAA-1. Examination of cross-reactivity of the mabs to human tissues using immunohistochemistry. Examination of reactivity to endometrial carcinoma cell lines and to PC-12 cells by immunohistochemistry and by radioimmunoassay.


RESULTS
Mabs to immunoaffinity-purified SAA-1 recognize three coprecipitating molecules. Cross-reactivity was demonstrated to glandular epithelia exhibiting ligand-induced exocytosis, and to endometrial carcinoma and PC-12 cells.


CONCLUSIONS
The proteins described could be components of the exocytosis-regulating machinery of human spermatozoa.
Brucker
C
C
I. Frauenklinik, Ludwig-Maximilians-Universität, München.
Herder
G v
Gv
Keberle
A
A
Kuhn
G
G
Schlamp
V
V
Lipford
G B
GB
D000177:Acrosome / Q000502:physiology*; D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D002868:Chromosomal Proteins, Non-Histone / Q000302:isolation & purification / Q000502:physiology*; D005089:Exocytosis / Q000502:physiology*; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011247:Pregnancy; D013084:Sperm-Ovum Interactions / Q000502:physiology*; D013094:Spermatozoa / Q000502:physiology*
D000911:Antibodies, Monoclonal; D002868:Chromosomal Proteins, Non-Histone; C053886:spermatid transition proteins
Spermatozoen-Antigen SAA-1 und kopräzipitierende Moleküle: Regulation der menschlichen Akrosomenreaktion.