Purification and some properties of hemagglutinin from the Myxomycete, Physarum polycephalum.
A new hemagglutinin was isolated from the plasmodium of Physarum polycephalum by salting out with ammonium sulphate followed by chromatography on DE-32, DEAE-Toyopearl and hydroxyapatite. This hemagglutinin, named physarumin, was purified 1000-fold over crude extracts. The molecular weight of physarumin was determined to be 22,000 by size exclusion chromatography on Bio-Gel P-60 and 8,700 by SDS-polyacrylamide gel electrophoresis. Physarumin agglutinated rabbit, guinea pig, horse and human erythrocytes. Physarumin-induced hemagglutination was inhibited by fetuin and alpha 1-acid glycoprotein, but not by commercially available mono- and disaccharides. Hemagglutinating activity was blocked by EDTA, and was restored by adding Ca2+ but not by Mg2+.
Yokota
M
M
Department of Health Sciences, Asahikawa College of Hokkaido University of Education, Japan.
Nitta
K
K
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002122:Calcium Chloride / Q000494:pharmacology; D002850:Chromatography, Gel; D004492:Edetic Acid / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D006168:Guinea Pigs; D006384:Hemagglutination; D006388:Hemagglutinins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D006736:Horses; D006801:Humans; D007526:Isoelectric Point; D037181:Lectins, C-Type; D015636:Magnesium Chloride / Q000494:pharmacology; D008970:Molecular Weight; D016839:Physarum polycephalum / Q000737:chemistry*; D011817:Rabbits
D000596:Amino Acids; D006388:Hemagglutinins; D037181:Lectins, C-Type; C100660:physarumin; D015636:Magnesium Chloride; D004492:Edetic Acid; D002122:Calcium Chloride; D002118:Calcium