title: Enhancing effects of vasoconstrictors on bile flow and bile acid excretion in the isolated perfused rat liver.
abstract: The effects of vasoconstrictors on bile flow and bile acid excretion were examined in single-pass isolated perfused rat livers. Administration of norepinephrine (NE), 4 nmol/min, plus continuous infusion of taurocholate (TC) (1.0 mumol/min) rapidly increased bile flow in 1 min, and from min 5 until the end of NE administration (late period) bile flow remained above the basal level (111.7 +/- 2.2%), as did bile acid output (114.6 +/- 1.8%). Without TC infusion, administration of NE produced no increase in the late period. Administration of NE plus taurochenodeoxycholate (1.0 mumol/min) increased bile flow and bile acid output in the late period to 121.9 +/- 7.0 and 137.1 +/- 6.8%, respectively. With NE plus taurodehydrocholate, the respective values were only 105.4 +/- 1.6 and 104.1 +/- 4.0%. When horseradish peroxidase (HRP) (25 mg) was infused over 1 min with continuous NE, the late peak (20-25 min) of HRP elimination into bile significantly exceeded that of untreated controls (P < 0.01). These observations suggest that vasoconstrictors enhance biliary excretion of more hydrophobic bile acids, in part by stimulating vesicular transport.
authors.affiliation: First Department of Internal Medicine, Nagoya City University Medical School, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Hoshino
authors.forename: A
authors.initials: A
authors.lastname: Tanaka
authors.forename: T
authors.initials: T
authors.lastname: Hayakawa
authors.forename: T
authors.initials: T
authors.lastname: Ohiwa
authors.forename: K
authors.initials: K
authors.lastname: Katagiri
authors.forename: M
authors.initials: M
authors.lastname: Miyaji
authors.forename: K
authors.initials: K
authors.lastname: Tsukada
authors.forename: T
authors.initials: T
authors.lastname: Takeuchi
mesh_terms: D000818:Animals; D001646:Bile / Q000502:physiology*; D001647:Bile Acids and Salts / Q000502:physiology*; D008099:Liver / Q000502:physiology*; D008297:Male; D009638:Norepinephrine / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D014661:Vasoconstriction / Q000187:drug effects / Q000502:physiology*
chemical_list: D001647:Bile Acids and Salts; D009638:Norepinephrine