mucS, a gene involved in activation of galactoglucan (EPS II) synthesis gene expression in Rhizobium meliloti.
In addition to the exopolysaccharide succinoglycan, Rhizobium meliloti can produce a galactoglucan exopolysaccharide, EPS II. The production of EPS II occurs in certain mutant strains, in strains containing extra copies of EPS II synthesis genes, or in the wild-type strain under phosphate-limiting conditions. We have identified a gene, mucS, that is in a locus required for EPS II induction by extra gene copies and by phosphate limitation, and that activates the expression of at least one other EPS II synthesis gene. mucS lies within a cluster of EPS II synthesis genes and contains an open reading frame of 190 amino acids. MucS does not show any significant similarity to known genes and may represent a new type of regulatory protein.
Astete
S G
SG
Department of Microbiology, University of Washington, Seattle, 98195, USA.
Leigh
J A
JA
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers; D004926:Escherichia coli; D005656:Fungal Proteins / Q000096:biosynthesis* / Q000378:metabolism; D005685:Galactans; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D005838:Genotype; D005936:Glucans; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D016133:Polymerase Chain Reaction; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000378:metabolism; D012091:Repetitive Sequences, Nucleic Acid; D016962:Sinorhizobium meliloti / Q000235:genetics / Q000378:metabolism*; D015534:Trans-Activators
D017931:DNA Primers; C100020:EXPG protein, Rhizobium meliloti; D005656:Fungal Proteins; D005685:Galactans; D005936:Glucans; D011135:Polysaccharides, Bacterial; D011993:Recombinant Fusion Proteins; D015534:Trans-Activators; C093768:galactoglucan