Involvement of interleukin-6 and interferon-alpha in the poly(A).Poly(U)-induced 2',5'-oligoadenylate synthetase activity in the mouse monocyte-macrophage cell line, J774A1.
The synthetic polyribonucleotide poly(A).poly(U) induces 2',5'-oligoadenylate synthetase activity in the murine macrophage cell line J774A1. The possible role of several cytokines involved in macrophage activation (i.e., IL-1, IL-6, TNF, and IFN) was examined in the present study. It was first demonstrated that among the anticytokine antibodies, only monoclonal antibodies directed against IL-6 inhibited the induction of 2',5'-oligoadenylate synthetase by poly(A).poly(U) in a dose-dependent manner. Moreover, it was established that poly(A).poly(U) elicited IL-6 production in J774A1 cells in a time-and dose-dependent manner. Consequently, the effect of IL-6 on 2',5'-oligoadenylate synthetase activity was studied. IL-6 either alone or in combination with IL-1 and TNF did not induce 2',5'-oligoadenylate synthetase activity. IL-6 did not potentiate IFN-gamma-induced 2'-5'-oligoadenylate synthetase activity. In contrast, addition of IL-6 to the incubation medium potentiated the stimulation of 2'-5'-oligoadenylate synthetase activity by IFN-alpha. These results suggest that IL-6 is a necessary but not sufficient factor in the induction of 2'-5'-oligoadenylate synthetase activity in the J774A1 cell line by poly(A).poly(U).
Marin
J G
JG
Institut Henri Beaufour, Les Ulis, France.
Mencia-Huerta
J M
JM
Braquet
P
P
Chabrier
P E
PE
D015088:2',5'-Oligoadenylate Synthetase / Q000096:biosynthesis*; D000818:Animals; D000906:Antibodies / Q000097:blood; D007369:Interferon Inducers / Q000494:pharmacology*; D016898:Interferon-alpha / Q000276:immunology / Q000502:physiology*; D007375:Interleukin-1 / Q000276:immunology / Q000502:physiology; D015850:Interleukin-6 / Q000276:immunology / Q000502:physiology*; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D011063:Poly A-U / Q000494:pharmacology*; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology / Q000502:physiology
D000906:Antibodies; D007369:Interferon Inducers; D016898:Interferon-alpha; D007375:Interleukin-1; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D011063:Poly A-U; D015088:2',5'-Oligoadenylate Synthetase