title: High resolution localization of cruzipain and Ssp4 in Trypanosoma cruzi by replica staining label fracture.
abstract: The replica staining label fracture technique was used to analyse the distribution of cruzipain and Ssp4 in Trypanosoma cruzi. Intense labeling for the two proteins was seen on the E fracture face of amastigote forms. Gold particles did not co-localize with the intramembranous particles. Labeling was abolished by previous treatment of the parasites with phospholipase C from Trypanosoma brucei, which removes glycosylphosphatidyl inositol (GPI) anchored proteins. These observations suggest that cruzipain and Ssp4 are attached to the parasite surface via a GPI anchor.
authors.affiliation: Laboratório de Imunopatologia Keizo Azami, Universidade Federal de Pernanbuco, Recife, Brasil.
authors.forename: A E
authors.initials: AE
authors.lastname: Nascimento
authors.forename: W
authors.initials: W
authors.lastname: de Souza
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan; D000918:Antibody Specificity; D003208:Concanavalin A; D003546:Cysteine Endopeptidases / Q000032:analysis* / Q000276:immunology; D005614:Freeze Fracturing; D006023:Glycoproteins / Q000032:analysis / Q000276:immunology; D006046:Gold; D008565:Membrane Proteins / Q000032:analysis / Q000276:immunology; D015800:Protozoan Proteins / Q000032:analysis* / Q000276:immunology; D014349:Trypanosoma cruzi / Q000737:chemistry* / Q000254:growth & development
chemical_list: D000913:Antibodies, Protozoan; D006023:Glycoproteins; D008565:Membrane Proteins; D015800:Protozoan Proteins; D003208:Concanavalin A; D006046:Gold; D003546:Cysteine Endopeptidases; C063214:cruzipain