Detection of antibodies to Staphylococcus aureus in water buffalo milk by flow cytometry.
An assay has been developed to detect antibodies to Staphylococcus aureus in water buffalo milk by flow cytometry. The method was the protein A-deficient strain Wood 46 of S aureus incubated with milk samples and fluorescein-labelled rabbit anti-water buffalo antiserum. The assay can detect antibodies when the pathogen is not detectable by bacterial tests and can determine the antibody titre directly on undiluted samples.
D'Apice
L
L
University of Naples, School of Agriculture, Italy.
Fenizia
D
D
Capparelli
R
R
Scala
F
F
Iannelli
D
D
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis*; D002020:Buffaloes; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D008892:Milk / Q000276:immunology* / Q000382:microbiology*; D011817:Rabbits; D012680:Sensitivity and Specificity; D013211:Staphylococcus aureus / Q000276:immunology*
D000907:Antibodies, Bacterial