Recombinant human erythropoietin improves enzyme activity in erythrocytes of predialysis uremics.
The influence of r-HuEPO on the activity of erythrocyte transketolase (ETKA), glutathione reductase (GSH) and glutamic-pyruvic transaminase (EGPT) was determined by spectrophotometry. 150 IU/kg BW/week of r-HuEPO was given i.v. during 2 months to 12 predialyzed uremics (PDU). Twenty healthy volunteers (HV) served as controls. GSH and EGPT activity were expressed as a coefficient. ETKA in HV = 2.39 +/- 0.10 and PDU = 1.53 +/- 0.10 mumol/ml/min differed significantly (p < 0.001). GSH in HV = 1.20 +/- 0.09 and PDU = 1.25 +/- 0.07, EGPT in HV = 1.20 +/- 0.10 and PDU = 1.38 +/- 0.11 differed significantly (p < 0.01 and p < 0.001, respectively). After 2 months of r-HuEPO treatment, ETKA, GSH and EGPT rose significantly (p < 0.01). Thus, r-HuEPO increases the activity of enzymes related with the content of vitamins B1, B2 and B6 in uremics.
Pietrzak
I
I
Department of Nephrology, University School of Medical Sciences, Poznan, Poland.
Baczyk
K
K
Kubiak
W
W
D000410:Alanine Transaminase / Q000097:blood*; D003404:Creatinine / Q000097:blood; D004912:Erythrocytes / Q000201:enzymology*; D004921:Erythropoietin / Q000494:pharmacology / Q000627:therapeutic use*; D005980:Glutathione Reductase / Q000097:blood*; D006400:Hematocrit; D006801:Humans; D011994:Recombinant Proteins / Q000494:pharmacology / Q000627:therapeutic use; D012016:Reference Values; D006435:Renal Dialysis; D014174:Transketolase / Q000097:blood*; D014511:Uremia / Q000097:blood* / Q000201:enzymology / Q000628:therapy*
D011994:Recombinant Proteins; D004921:Erythropoietin; D003404:Creatinine; D005980:Glutathione Reductase; D014174:Transketolase; D000410:Alanine Transaminase