BCR/ABL modulates the cytokine and retinoic acid response of c-Rel in human myeloid cells.
A human myeloid cell line, Mo7, and a daughter cell line expressing the bcr/abl oncogene, Mo7-P210, were used in a comparative study analyzing the effects of p210BCR/ABL expression on tyrosine phosphorylation, specific DNA binding and expression of the proto-oncoprotein c-Rel. The steady state expression of c-Rel was indistinguishable in both cell lines. Tyrosine phosphorylation and DNA binding of c-Rel were slightly elevated in Mo7-P210 cells. Further, Mo7 and Mo7-P210 cells showed different responses concerning c-Rel after stimulation with cytokines and retinoic acid. The results presented here demonstrate that c-Rel can be modulated by hematopoietic cytokines and suggest that bcr/abl expression has an impact on these responses and that c-Rel may be a downstream effector for p210BCR/ABL.
Neumann
M
M
Institute of Pathology, University of Wurzburg, Germany.
Wilisch
A
A
Ma
B C
BC
Druker
B J
BJ
Serfling
E
E
Franza
B R
BR
D001483:Base Sequence; D016207:Cytokines / Q000494:pharmacology*; D004273:DNA, Neoplasm / Q000378:metabolism; D016044:Fusion Proteins, bcr-abl / Q000378:metabolism / Q000502:physiology*; D006801:Humans; D007947:Leukemia, Megakaryoblastic, Acute / Q000378:metabolism*; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000378:metabolism; D010766:Phosphorylation; D011518:Proto-Oncogene Proteins / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology*; D020569:Proto-Oncogene Proteins c-rel; D015398:Signal Transduction / Q000502:physiology; D014212:Tretinoin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000187:drug effects; D014443:Tyrosine / Q000378:metabolism
D016207:Cytokines; D004273:DNA, Neoplasm; D009363:Neoplasm Proteins; D011518:Proto-Oncogene Proteins; D020569:Proto-Oncogene Proteins c-rel; D014443:Tyrosine; D014212:Tretinoin; D016044:Fusion Proteins, bcr-abl