title: CTL lysis: there is a hyperbolic relation of killing rate to exocytosable granzyme A for highly cytotoxic murine cytotoxic T lymphocytes.
abstract: The lysis of susceptible targets by efficient cytotoxic T lymphocytes (CTL) increases both with time and with the ratio of CTL to target. Simple methods for calculating a killing rate constant from the time dependence of killing and for calculating the relation of the killing rate constant to the concentration of exocytosable granzyme A are given. Application of these methods to the killing of target cells by the highly efficient mouse CTL AR1 is presented. AR1 needed granzyme A for efficient killing. AR1 contained sufficient exocytosable granzyme A to kill at about 80% of the rate possible at infinite exocytosable granzyme A.
authors.affiliation: Department of Immunology Research, Merck Research Laboratories, Rahway, NJ 07065-0900, USA.
authors.forename: M
authors.initials: M
authors.lastname: Poe
authors.forename: J K
authors.initials: JK
authors.lastname: Wu
authors.forename: A
authors.initials: A
authors.lastname: Talento
authors.forename: G C
authors.initials: GC
authors.lastname: Koo
mesh_terms: D000818:Animals; D002460:Cell Line; D003601:Cytotoxicity Tests, Immunologic; D003602:Cytotoxicity, Immunologic; D005089:Exocytosis / Q000276:immunology*; D053804:Granzymes; D007700:Kinetics; D051379:Mice; D012697:Serine Endopeptidases / Q000276:immunology / Q000378:metabolism*; D013602:T-Lymphocytes, Cytotoxic / Q000201:enzymology* / Q000276:immunology*
chemical_list: D053804:Granzymes; D012697:Serine Endopeptidases