Antenatal glucocorticoid treatment attenuates immediate postnatal prostacyclin and thromboxane levels in plasma of very preterm lambs.
The effect of antenatal glucocorticoid treatment on the production of cyclooxygenase metabolites was studied in very preterm lambs. Seven fetal lambs, 121 days of gestation, received a single dose of betamethasone, 0.5 mg/kg i.m., 48 h prior to delivery. Five age-matched controls received saline intramuscularly. Each fetus was delivered and ventilated for 3 h and sacrificed. Plasma was prepared from blood drawn from the umbilical cord of each fetus, and 60, 120 and 180 min after delivery. Mesenteric (MESA) and femoral (FEMA) arteries were isolated and incubated in Krebs' buffer for 10 min at 37 degrees C. Samples were extracted for prostacyclin (PGI2), and thromboxane (Tx)A2, purified by HPLC and measured by specific radioimmunoassay. Amounts of metabolites measured postnatally from betamethasone-treated preterm lambs were significantly lower (p < 0.05) than the amounts from saline lambs. Prostacyclin production by MESA and FEMA of betamethasone-treated lambs was lower than by vessels of saline-treated lambs. There was no difference in TxA2 production by vessels from the two groups of preterm lambs. Our data show that antenatal betamethasone treatment decreased systemic prostanoid production suggesting a decreased reactivity of the vascular membrane.
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Department of Pediatrics, Harbor-UCLA Medical Center, Torrance 90502, USA.
Okogbule-Wonodi
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Raj
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D000818:Animals; D000831:Animals, Newborn / Q000097:blood*; D001623:Betamethasone / Q000008:administration & dosage / Q000494:pharmacology*; D002245:Carbon Dioxide / Q000097:blood; D015232:Dinoprostone / Q000096:biosynthesis; D011464:Epoprostenol / Q000096:biosynthesis / Q000097:blood*; D005260:Female; D005263:Femoral Artery / Q000187:drug effects / Q000378:metabolism; D005865:Gestational Age; D005938:Glucocorticoids / Q000008:administration & dosage / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D008638:Mesenteric Arteries / Q000187:drug effects / Q000378:metabolism; D010100:Oxygen / Q000097:blood; D011247:Pregnancy; D012756:Sheep; D013928:Thromboxane A2 / Q000096:biosynthesis / Q000097:blood*
D005938:Glucocorticoids; D002245:Carbon Dioxide; D013928:Thromboxane A2; D001623:Betamethasone; D011464:Epoprostenol; D015232:Dinoprostone; D010100:Oxygen