The s29x gene of symbiotic bacteria in Amoeba proteus with a novel promoter.
Gram-symbiotic bacteria (called X-bacteria), present in the xD strain of Amoeba proteus as required cell components, synthesize and export a large amount of a 29-kDa protein, S29x. S29x is exported into the host's cytoplasm across the bacterial membranes and the symbiosome membrane. The complete nucleotide (nt) sequence of the s29x gene of X-bacteria has been determined, and the promoter sequence and tsp have also been identified. The gene has a nonconventional promoter with putative nt sequences different from the known consensus sequences. When Escherichia coli cells are transformed with s29x, the gene is expressed and the product is secreted into the culture medium. Functions of S29x are not fully known, but it is suspected that S29x plays an important role in the symbiotic relationship between amoebae and X-bacteria.
Pak
J W
JW
Cell Biology Laboratory, BCMB, University of Tennessee, Knoxville 37996, USA.
Jeon
K W
KW
D000595:Amino Acid Sequence; D000656:Amoeba / Q000382:microbiology*; D000818:Animals; D001426:Bacterial Proteins; D001483:Base Sequence; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial / Q000235:genetics*; D006090:Gram-Negative Bacteria / Q000235:genetics*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011401:Promoter Regions, Genetic / Q000235:genetics*; D021382:Protein Sorting Signals / Q000235:genetics; D015800:Protozoan Proteins / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000737:chemistry; D017422:Sequence Analysis, DNA; D013559:Symbiosis; D014158:Transcription, Genetic / Q000235:genetics
D001426:Bacterial Proteins; D021382:Protein Sorting Signals; D015800:Protozoan Proteins; D011993:Recombinant Fusion Proteins; C099986:S29X protein, Amoeba proteus symbiotic bacterium