Phosphatase 2A regulates the responsiveness of olfactory cilia.
The odorant-induced second messenger response in olfactory cilia was monitored in the presence of phosphatase modulators. Okadaic acid, a phosphatase inhibitor, attenuated the odorant-induced cAMP-response in a dose-dependent manner, half maximal inhibition was obtained at 1.5 nM okadaic acid indicating that phosphatase 2A may be involved. Protamine, a selective activator of phosphatase 2A, led to significantly stronger cAMP-responses. Western blot and immunohistochemical analysis employing specific antibodies revealed that phosphatase 2A is present in olfactory tissues in particular in olfactory cilia. The results suggest that phosphatase 2A may play a regulatory role in governing the responsiveness of olfactory neurons.
Kroner
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C
Institute of Zoophysiology, University Stuttgart-Hohenheim, Germany.
Boekhoff
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I
Breer
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H
D000818:Animals; D000906:Antibodies / Q000276:immunology; D015153:Blotting, Western; D002923:Cilia / Q000187:drug effects / Q000201:enzymology*; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000378:metabolism / Q000494:pharmacology; D004988:Ethers, Cyclic / Q000494:pharmacology; D007150:Immunohistochemistry; D009812:Odorants; D019319:Okadaic Acid; D018034:Olfactory Receptor Neurons / Q000187:drug effects / Q000201:enzymology*; D010749:Phosphoprotein Phosphatases / Q000378:metabolism*; D010766:Phosphorylation; D011479:Protamines / Q000494:pharmacology; D054648:Protein Phosphatase 2; D051381:Rats; D017207:Rats, Sprague-Dawley; D015290:Second Messenger Systems / Q000502:physiology; D015398:Signal Transduction / Q000502:physiology*
D000906:Antibodies; D004988:Ethers, Cyclic; D011479:Protamines; D005576:Colforsin; D019319:Okadaic Acid; D000242:Cyclic AMP; D010749:Phosphoprotein Phosphatases; D054648:Protein Phosphatase 2