[Antitumor effect of interleukin-2 gene-transfected tumor vaccine in combination with interleukin-6 gene-transfected tumor vaccine].
It has been shown that IL-2 and IL-6 can cooperate to induce immune responses more effectively. In order to increase the therapeutic efficacy of cytokine gene-transfected tumor vaccines, we treated the experimental pulmonary metastatic melanoma-bearing mice with IL-2 gene-transfected tumor vaccine and IL-6 gene-transfected tumor vaccine. After the combined treatment, the pulmonary metastases were reduced more significantly and the survival time of tumor-bearing mice was also prolonged more significantly. The CTL activity, NK activity and IL-2-induced LAK activity, IL-2 and TNF secretion from the splenocytes of the above tumor-bearing mice increased more significantly than that of tumor-bearing mice treated with IL-2 gene-transfected vaccine or IL-6 gene-transfected vaccine alone. These results demonstrated that the combined use of IL-2 gene-transfected tumor vaccine and IL-6 gene-transfected tumor vaccine could achieve more potent antitumor effect via more efficient activation of immune functions. The experiment outlines a novel approach to the cytokine gene therapy of cancer.
Cao
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X
Department of Immunology, Second Military Medical University, Shanghai.
Zhang
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W
Tao
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Q
D000818:Animals; D015316:Genetic Therapy; D007376:Interleukin-2 / Q000235:genetics*; D015850:Interleukin-6 / Q000235:genetics*; D008175:Lung Neoplasms / Q000556:secondary / Q000628:therapy*; D008297:Male; D008546:Melanoma, Experimental / Q000556:secondary / Q000628:therapy*; D051379:Mice; D008810:Mice, Inbred C57BL; D014162:Transfection; D014407:Tumor Cells, Cultured
D007376:Interleukin-2; D015850:Interleukin-6