Structure of the ripple phase in lecithin bilayers.
The phases of the x-ray form factors are derived for the ripple (Pbeta') thermodynamic phase in the lecithin bilayer system. By combining these phases with experimental intensity data, the electron density map of the ripple phase of dimyristoyl-phosphatidylcholine is constructed. The phases are derived by fitting the intensity data to two-dimensional electron density models, which are created by convolving an asymmetric triangular ripple profile with a transbilayer electron density profile. The robustness of the model method is indicated by the result that many different models of the transbilayer profile yield essentially the same phases, except for the weaker, purely ripple (0,k) peaks. Even with this residual ambiguity, the ripple profile is well determined, resulting in 19 angstroms for the ripple amplitude and 10 degrees and 26 degrees for the slopes of the major and the minor sides, respectively. Estimates for the bilayer head-head spacings show that the major side of the ripple is consistent with gel-like structure, and the minor side appears to be thinner with lower electron density.
Sun
W J
WJ
Department of Physics, Carnegie Mellon University, Pittsburg, PA 15213, USA.
Tristram-Nagle
S
S
Suter
R M
RM
Nagle
J F
JF
D004583:Electrons; D008051:Lipid Bilayers / Q000737:chemistry*; D008433:Mathematics; D008958:Models, Molecular; D008962:Models, Theoretical; D010713:Phosphatidylcholines / Q000737:chemistry*; D014961:X-Ray Diffraction
D008051:Lipid Bilayers; D010713:Phosphatidylcholines