Organization and chromosomal localization of a B1-like containing repeat of Microtus subarvalis.
A repetitive DNA sequence, MS2, was isolated from EcoRI-digested genomic DNA of the vole, Microtus subarvalis. The fragment was cloned and sequenced. Sequence analysis of this 1194-bp fragment revealed a 156-bp region demonstrating a 55% homology with the mouse B1 repeat. The remaining MS2 sequence shows no significant homology with other known GenBank sequences. The results of in situ hybridization of MS2 on vole metaphase chromosomes indicate the fragment is confined to heterochromatin blocks of the sex chromosomes in all but one species (M. arvalis). Distribution of MS2 sequences provides evidence for heterogeneity of the giant heterochromatin blocks of the XY Chromosomes (Chrs) in voles, for the unique cluster-like localization of MS2 within these blocks.
Mayorov
V I
VI
Institute of Cytology and Genetics, Siberian Department of the Russian Academy of Sciences, Novosibirsk Russia 630090, USA.
Adkison
L R
LR
Vorobyeva
N V
NV
Khrapov
E A
EA
Kholodhov
N G
NG
Rogozin
I B
IB
Nesterova
T B
TB
Protopopov
A I
AI
Sablina
O V
OV
Graphodatsky
A S
AS
Zakian
S M
SM
D000595:Amino Acid Sequence; D000818:Animals; D003411:Arvicolinae / Q000235:genetics*; D001483:Base Sequence; D002871:Chromosome Banding; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D016384:Consensus Sequence; D015246:Deoxyribonuclease EcoRI; D005260:Female; D006570:Heterochromatin; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D014960:X Chromosome; D014998:Y Chromosome
D006570:Heterochromatin; D015246:Deoxyribonuclease EcoRI