[Do cell cycle changes of human ovarian carcinoma cells after exposure to cytostatic drugs in vitro correlate with cytotoxicity?].
OBJECTIVE
Is there a correlation between cell cycle perturbations and cytotoxicity in ovarian cancer cells exposed to cytotoxic drugs in vitro?


METHODS
We tested the association between cytotoxicity and in vitro cell cycle perturbations after exposure of human ovarian cancer cells to seven cytotoxic agents.


RESULTS
Three principal patterns of cell cycle alterations were observed; a sequential S-G/M block after exposure to the non phase-specific agents cis-platinum, 4-hydroperoxy-eyclophosphamide, and mitomycin C (Group I); an isolated G2/M block after exposure to the G2/M phase-specific drugs etoposide and vincristine (Group II); and an isolated S block following exposure to the S phase-specific agents 5-fluorouracil and cytosine arabinoside (Group III). Overall, there was no direct correlation between the degree of cell cycle perturbations and cytotoxicity. However, when the three subgroups of phase non-specific agents, S phase-specific agents, and G2/M specific agents were analyzed separately, positive correlations between the magnitude of cell kinetic alterations and cytotoxicity were observed.


CONCLUSIONS
Cell kinetic alterations appeared to precede cytotoxicity. The experimental model may be particularly useful to study cell kinetic effects after high-dose chemotherapy since, in contrast to conventional chemotherapy, the magnitude of cell kinetic perturbations after this kind of treatment is significantly increased.
Petru
E
E
Geburtshilflich-Gynäkol. Univ. Klinik Graz.
Haas
J
J
Schaider
H
H
Sevin
B U
BU
D000970:Antineoplastic Agents / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D010051:Ovarian Neoplasms / Q000473:pathology*; D014407:Tumor Cells, Cultured / Q000187:drug effects* / Q000473:pathology
D000970:Antineoplastic Agents
Korrelieren Zellzyklus-Veränderungen von humanen Ovarialkarzinomzellen nach Exposition gegenüber Zytostatika in vitro mit der Zytotoxizität?