RN-tre identifies a family of tre-related proteins displaying a novel potential protein binding domain.
Eps8 is a recently identified SH3-containing substrate for tyrosine kinase receptors. To understand the role of eps8 in receptor-mediated signaling, we cloned cDNAs encoding proteins that bind to its SH3 domain. One of these cDNAs predicts the synthesis of an 828 amino acid protein with homology to the N-terminal region of the tre oncogene. We designated this protein RN-tre for Related to the N-terminus of tre. RN-tre is ubiquitously expressed and maps to 10p13, a region known to be involved in translocations in various leukemias. In addition, a 10p13 monosomy syndrome, characterized by developmental alterations, has been reported. The regional homology between RN-tre and tre, which is limited to their N-terminal portion, prompted us to investigate the origin of the tre oncogene transcriptional unit. We were able to show that tre is the fusion product of a 5' genetic element, homologous to RN-tre and a 3' element, encoding a de-ubiquinating enzyme. Moreover, we identified, within the N-terminus of RN-tre and tre, a domain (named TrH, for Tre Homology), which is conserved within several proteins from yeast to mammals and has protein-binding properties in vitro.
Matòsková
B
B
IEO, European Institute of Oncology, Milan, Italy.
Wong
W T
WT
Seki
N
N
Nagase
T
T
Nomura
N
N
Robbins
K C
KC
Di Fiore
P P
PP
D016475:3T3 Cells; D048868:Adaptor Proteins, Signal Transducing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002874:Chromosome Mapping; D002879:Chromosomes, Human, Pair 10; D017124:Conserved Sequence; D003598:Cytoskeletal Proteins; D018076:DNA, Complementary / Q000096:biosynthesis / Q000302:isolation & purification; D010450:Endopeptidases; D019143:Evolution, Molecular; D020690:GTPase-Activating Proteins / Q000235:genetics*; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D051379:Mice; D008969:Molecular Sequence Data; D015513:Oncogene Proteins; D015514:Oncogene Proteins, Fusion / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D011506:Proteins / Q000235:genetics / Q000378:metabolism; D011518:Proto-Oncogene Proteins; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic; D043222:Ubiquitin Thiolesterase; D018909:src Homology Domains
D048868:Adaptor Proteins, Signal Transducing; D003598:Cytoskeletal Proteins; D018076:DNA, Complementary; D010450:Endopeptidases; D020690:GTPase-Activating Proteins; D047908:Intracellular Signaling Peptides and Proteins; D015513:Oncogene Proteins; D015514:Oncogene Proteins, Fusion; D011506:Proteins; D011518:Proto-Oncogene Proteins; D011993:Recombinant Fusion Proteins; D043222:Ubiquitin Thiolesterase; C489989:EPS8 protein, human; C083430:Eps8 protein, mouse; C000590733:Usp6nl protein, Mouse; C100665:USP6NL protein, human; C473330:USP6 protein, human