Exclusion of Int-6 from PML nuclear bodies by binding to the HTLV-I Tax oncoprotein.
The Tax transactivator of the human T cell leukemia virus type I (HTLV-I) exhibits oncogenic properties. A screen for proteins interacting with Tax yielded a complementary DNA (cDNA) encoding the human Int-6 protein. In mice, the Int-6 gene can be converted into a putative dominant negative oncogene after retroviral insertion. Here, Int-6 was localized in the cell nucleus to give a speckled staining pattern superposed to that of the promyelocytic leukemia (PML) protein. The binding of Tax to Int-6 caused its redistribution from the nuclear domains to the cytoplasm. Thus, Int-6 is a component of the PML nuclear bodies and Tax disrupts its normal cellular localization by binding to it.
Desbois
C
C
Centre National de la Recherche Scientifique UMR49, Ecole Normale Supérieure de Lyon, 69364 Lyon Cedex 07, France.
Rousset
R
R
Bantignies
F
F
Jalinot
P
P
D000818:Animals; D002460:Cell Line; D002467:Cell Nucleus / Q000737:chemistry*; D003593:Cytoplasm / Q000737:chemistry; D039621:Eukaryotic Initiation Factor-3; D016356:Gene Products, tax / Q000032:analysis / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D009363:Neoplasm Proteins; D009687:Nuclear Proteins; D000071156:Promyelocytic Leukemia Protein; D011518:Proto-Oncogene Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D014157:Transcription Factors / Q000032:analysis*; D014162:Transfection; D025521:Tumor Suppressor Proteins
D039621:Eukaryotic Initiation Factor-3; D016356:Gene Products, tax; D009363:Neoplasm Proteins; D009687:Nuclear Proteins; C513293:Pml protein, mouse; D000071156:Promyelocytic Leukemia Protein; D011518:Proto-Oncogene Proteins; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors; D025521:Tumor Suppressor Proteins; C070576:PML protein, human