Cyclic AMP inhibits expression of D-type cyclins and cdk4 and induces p27Kip1 in G-CSF-treated NFS-60 cells.
The addition of cAMP inhibits G-CSF-mediated proliferation and suppresses pRB phosphorylation in NFS-60 cells. We show that the latter could be attributed to different effects of cAMP in these cells: (i) down-regulation of the levels of cyclins D2 and D3, and cdk4, and (ii) induction of the p27Kip1 inhibitor of cdk4.
Ward
A C
AC
Department of Medicine, Royal Melbourne Hospital, University of Melbourne, Parkville, Victoria, Australia.
Csar
X F
XF
Hoffmann
B W
BW
Hamilton
J A
JA
D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000031:analogs & derivatives / Q000494:pharmacology*; D000818:Animals; D015152:Blotting, Northern; D018797:Cell Cycle Proteins; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D000242:Cyclic AMP / Q000031:analogs & derivatives / Q000494:pharmacology*; D056741:Cyclin D; D051358:Cyclin-Dependent Kinase 4; D050760:Cyclin-Dependent Kinase Inhibitor p27; D018844:Cyclin-Dependent Kinases / Q000096:biosynthesis*; D016213:Cyclins / Q000096:biosynthesis*; D004247:DNA / Q000096:biosynthesis; D015232:Dinoprostone / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D016147:Genes, Tumor Suppressor; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D007700:Kinetics; D008869:Microtubule-Associated Proteins / Q000096:biosynthesis*; D010766:Phosphorylation; D011518:Proto-Oncogene Proteins; D025521:Tumor Suppressor Proteins
D018797:Cell Cycle Proteins; D056741:Cyclin D; D016213:Cyclins; D008869:Microtubule-Associated Proteins; D011518:Proto-Oncogene Proteins; D025521:Tumor Suppressor Proteins; D016179:Granulocyte Colony-Stimulating Factor; D050760:Cyclin-Dependent Kinase Inhibitor p27; D015124:8-Bromo Cyclic Adenosine Monophosphate; D004247:DNA; D000242:Cyclic AMP; D051358:Cyclin-Dependent Kinase 4; D018844:Cyclin-Dependent Kinases; D015232:Dinoprostone; D015056:1-Methyl-3-isobutylxanthine