title: Genotypic diversity revealed by allozymes and oligonucleotide DNA fingerprinting in French populations of the aquatic macrophyte Sparganium erectum.
abstract: The vast majority of perennial plants reproduce sexually and vegetatively at the same time. This may lead to important variation among clonal plant populations in their amount of genotypic diversity. In order to verify this assumption, we compare the clonal diversity of 10 natural populations of the aquatic clonal macrophyte Sparganium erectum in France. Diversity was quantified by DNA fingerprinting and allozyme electrophoresis for a sample of 10 shoots per population. Two DNA probes (CA)8 and (TAA)6TA, were selected among 10 synthetic oligonucleotide probes containing simple repeat motifs. Both allozymes and DNA fingerprints revealed different amounts of diversity among populations. Five populations consist of a single genotype, whereas two populations were genetically highly diverse. In four of the monomorphic populations, absence of fingerprints diversity was combined with uniformly heterozygous allozyme loci, suggesting that each population was composed of a single clone. In the highly diverse populations, the level of clonal diversity combined with the allele segregation of the two allozyme loci Lap and Est suggests frequent seedling recruitment. The origin of new genotypes remains unclear but the absence of widespread genotypes together with the discrete distribution of Sparganium erectum populations implies that new genotypes are locally produced through sexual reproduction.
authors.affiliation: Génétique et Evolution des Populations Végétales, URA CNRS 1185 - Université de LILLE I, Villeneuve d'Ascq, France, Piquot@univ-lille1.fr
authors.forename: Y
authors.initials: Y
authors.lastname: Piquot
authors.forename: P
authors.initials: P
authors.lastname: Saumitou-LaPrade
authors.forename: D
authors.initials: D
authors.lastname: Petit
authors.forename: P
authors.initials: P
authors.lastname: Vernet
authors.forename: J T
authors.initials: JT
authors.lastname: Epplen
mesh_terms: D001483:Base Sequence; D016172:DNA Fingerprinting; D018744:DNA, Plant / Q000235:genetics*; D017753:Ecosystem; D005602:France; D014644:Genetic Variation; D005838:Genotype; D008386:Marine Biology; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes / Q000235:genetics; D010944:Plants / Q000201:enzymology* / Q000235:genetics*
chemical_list: D018744:DNA, Plant; D015345:Oligonucleotide Probes