Bone chondroadherin promotes attachment of osteoblastic cells to solid-state substrates and shows affinity to collagen.
Chondroadherin, which is reported to be synthesized by chondrocytes and to promote their attachment, was purified from bovine bone. It was a minor component of bone organic matrix, and was present in the 4 M guanidine extract of demineralized bone. Chondroadherin promoted attachment of osteoblastic cells to solid-state substrates, and bound to collagen. Binding of chondroadherin to collagen was significantly higher than that of osteonectin or decorin. These findings imply that chondroadherin may play a role in maintaining bone cells on the collagen matrices of bone.
Mizuno
M
M
Department of Biochemistry, School of Dentistry Hokkaido University, Sapporo Japan 060.
Fujisawa
R
R
Kuboki
Y
Y
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D015153:Blotting, Western; D001842:Bone and Bones / Q000737:chemistry* / Q000166:cytology / Q000378:metabolism; D002417:Cattle; D002448:Cell Adhesion; D003094:Collagen / Q000378:metabolism*; D058575:Decorin; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D016326:Extracellular Matrix Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000502:physiology*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism*; D015676:Osteonectin / Q000378:metabolism; D011485:Protein Binding; D011509:Proteoglycans / Q000378:metabolism; D012710:Serum Albumin, Bovine / Q000378:metabolism
D000596:Amino Acids; D058575:Decorin; D016326:Extracellular Matrix Proteins; D015676:Osteonectin; D011509:Proteoglycans; C088980:chondroadherin; D012710:Serum Albumin, Bovine; D003094:Collagen