Induction of microsomal enzymes in liver of rats treated with cyclohexanol.
The S9 fraction obtained from rats orally pretreated for 3 days with cyclohexanol was able to activate the pro-mutagen N-nitrosodimethylamine (NDMA) into highly mutagenic metabolite(s) detected in the TA100 strain of Salmonella typhimurium. NDMA was not mutagenic when uninduced S9 was used as metabolic source but was approximately twice more mutagenic with cyclohexanol-induced S9 compared to ethanol-induced S9. Separation of microsomal proteins by sodium dodecylsulfate gel electrophoresis, displayed protein bands situated in the range of 50,000 to 52,000 molecular weight induced by both, ethanol and cyclohexanol. These results are evidence of the induction properties of cyclohexanol.
Espinosa-Aguirre
J J
JJ
Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, México, D.F. jjea@servidor.unam.mx
Rubio
J
J
Cassani
M
M
Nosti
R
R
Caballero
S
S
González
I
I
Martínez
G
G
D000818:Animals; D003511:Cyclohexanols / Q000494:pharmacology*; D003577:Cytochrome P-450 Enzyme System / Q000187:drug effects* / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004790:Enzyme Induction / Q000187:drug effects; D000431:Ethanol / Q000633:toxicity; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology*; D009153:Mutagens / Q000633:toxicity; D009603:Nitroso Compounds / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics
D003511:Cyclohexanols; D009153:Mutagens; D009603:Nitroso Compounds; C008651:4-nitrosodimethylaniline; D000431:Ethanol; D003577:Cytochrome P-450 Enzyme System