title: Inhibition by verapamil of cholecystokinin-enhancement of pancreatic carcinogenesis induced by azaserine in Wistar rats.
abstract: The effect of a calcium channel blocker, verapamil, on cholecystokinin (CCK)-enhancement of pancreatic carcinogenesis induced by azaserine was investigated in Wistar rats. During and after 25 weekly injections of azaserine, each rat received alternate-day injections of CCK-octapeptide (CCK-8) and/or verapamil. Carcinogen-induced pancreatic lesions staining for mu class glutathione S-transferase (GST-mu) were examined histochemically at week 62. Prolonged administration of CCK-8 significantly increased the number and area as a percentage of parenchyma of GST-mu-positive lesions. Concomitant administration of verapamil significantly attenuated the enhancing effect of CCK-8. These findings indicate that calcium may play an important role in CCK-enhancement of pancreatic carcinogenesis.
authors.affiliation: Department of Gastrointestinal Oncology, Osaka Medical Center for Cancer and Cardiovascular Diseases, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Nakaizumi
authors.forename: H
authors.initials: H
authors.lastname: Uehara
authors.forename: M
authors.initials: M
authors.lastname: Baba
authors.forename: H
authors.initials: H
authors.lastname: Iishi
authors.forename: M
authors.initials: M
authors.lastname: Tatsuta
mesh_terms: D000818:Animals; D001377:Azaserine / Q000008:administration & dosage*; D002121:Calcium Channel Blockers / Q000494:pharmacology; D002766:Cholecystokinin / Q000008:administration & dosage*; D004357:Drug Synergism; D005982:Glutathione Transferase / Q000378:metabolism; D008297:Male; D010190:Pancreatic Neoplasms / Q000139:chemically induced*; D051381:Rats; D017208:Rats, Wistar; D014700:Verapamil / Q000008:administration & dosage*
chemical_list: D002121:Calcium Channel Blockers; D001377:Azaserine; D002766:Cholecystokinin; D014700:Verapamil; D005982:Glutathione Transferase