title: The effect of hydrocortisone and thyroxine on development of calcium homeostasis in embryonic intestinal epithelium.
abstract: Cytoplasmic Ca2+ concentration of epithelial cells from 14-day embryonic chick duodena decreased during 72 h of organ culture to a value 54% of that found at 17 days in vivo. The ability of cells to maintain a constant Ca2+ concentration when challenged with high extracellular calcium was also significantly reduced. Addition of 1 microM hydrocortisone during culture restored both parameters of Ca2+ homeostasis to that of 16-day uncultured duodena, and rise in cytoplasmic Ca2+ was significant within 4 h of hormone treatment. Thyroxine influenced epithelial Ca2+ similarly, but to a lesser degree and only after 48-72 h of culture. These data indicate that glucocorticoids, and possibly thyroid hormones, influence the development of calcium homeostasis in intestinal epithelium.
authors.affiliation: Department of Zoology, North Carolina State University, Raleigh 27695-7617, USA.
authors.forename: J O
authors.initials: JO
authors.lastname: Rogers
authors.forename: B L
authors.initials: BL
authors.lastname: Black
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002642:Chick Embryo; D003593:Cytoplasm / Q000378:metabolism; D004386:Duodenum / Q000187:drug effects / Q000196:embryology* / Q000378:metabolism; D004848:Epithelium / Q000187:drug effects / Q000196:embryology / Q000378:metabolism; D006706:Homeostasis / Q000187:drug effects*; D006854:Hydrocortisone / Q000494:pharmacology*; D009924:Organ Culture Techniques; D013974:Thyroxine / Q000494:pharmacology*; D013997:Time Factors
chemical_list: D013974:Thyroxine; D002118:Calcium; D006854:Hydrocortisone