title: NF-kappa B is induced in the nuclei of cultured rat aortic smooth muscle cells by stimulation of various growth factors.
abstract: We investigated whether induction of transcription factor NF-kappa B is involved in the proliferation of cultured rat aortic smooth muscle cell using electrophoretic mobility shift assay and immunocytochemistry. NF-kappa B was induced in the nucleus in a dose-dependent manner when the smooth muscle cells were stimulated by various growth factors such as PDGF-BB, bFGF, EGF and IGF-1, but not growth inhibitors such as TGF-beta and IFN-gamma. Among growth factors, PDGF-BB and bFGF, more potent growth stimulators, induced higher kappa B binding activity than EGF or IGF-1. These evidences were also supported by the results obtained with immunocytochemistry. Immunocytochemistry also showed that the induced NF-kappa B contained p50 and p65. These results suggest that NF-kappa B induction may be involved in the proliferation of vascular smooth muscle cell.
authors.affiliation: First Department of Internal Medicine, Faculty of Medicine, Kagoshima University, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Obata
authors.forename: S
authors.initials: S
authors.lastname: Biro
authors.forename: N
authors.initials: N
authors.lastname: Arima
authors.forename: H
authors.initials: H
authors.lastname: Kaieda
authors.forename: T
authors.initials: T
authors.lastname: Kihara
authors.forename: H
authors.initials: H
authors.lastname: Eto
authors.forename: M
authors.initials: M
authors.lastname: Miyata
authors.forename: H
authors.initials: H
authors.lastname: Tanaka
mesh_terms: D000818:Animals; D001011:Aorta; D001483:Base Sequence; D002455:Cell Division / Q000187:drug effects; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism*; D002478:Cells, Cultured; D017931:DNA Primers; D015342:DNA Probes; D004742:Enhancer Elements, Genetic; D006133:Growth Substances / Q000494:pharmacology*; D006801:Humans; D008969:Molecular Sequence Data; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D016328:NF-kappa B / Q000096:biosynthesis*; D009838:Oligodeoxyribonucleotides; D051381:Rats; D015375:Receptors, Interleukin-2 / Q000096:biosynthesis
chemical_list: D017931:DNA Primers; D015342:DNA Probes; D006133:Growth Substances; D016328:NF-kappa B; D009838:Oligodeoxyribonucleotides; D015375:Receptors, Interleukin-2