Isolation and in vitro propagation of human choroidal fibroblasts. Morphology and characterization of the cultures.
Subretinal ingrowth of choroidal fibroblasts is of importance in several clinical settings such as age-related macular degeneration or after traumas such as laser treatment and infection. In the present investigation we describe a method for isolation and propagation of human choroidal fibroblasts in vitro. Outgrowth from the outseeded choroid was studied by means of phase contrast microscopy, scanning electron microscopy and by immunohistochemical methods. The secondary cultures were found to contain almost only fibroblasts (more than 95% of the cells), some contaminating retinal pigment cells were also found. Choroidal fibroblasts contribute to the fibrovascular subretinal scarring process, and our cell culturing system seems to be a suitable tool for studying factors regulating the behavior of these cells in vitro.
Klyve
P
P
Department of Ophthalmology, Ullevål University Hospital, University of Oslo, Norway.
Nicolaissen
B
B
Naess
O
O
D000368:Aged; D000369:Aged, 80 and over; D002455:Cell Division; D002465:Cell Movement; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D002829:Choroid / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure; D003598:Cytoskeletal Proteins / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure; D006801:Humans; D007124:Immunoenzyme Techniques; D008855:Microscopy, Electron, Scanning; D008858:Microscopy, Phase-Contrast; D008875:Middle Aged
D003598:Cytoskeletal Proteins