Specific binding of allergenic soybean protein Gly m Bd 30K with alpha'- and alpha-subunits of conglycinin in soy milk.
When defatted soy milk was ultracentrifuged, 34kDa allergenic soyabean protein Gly m Bd 30 K was more abundant in the precipitate than in the supernatant by an SDS-PAGE analysis. The addition of more than 10 mM of 2-mercaptoethanol (2-ME) to the soy milk resulted not only in further removal of the 34kDa allergenic protein to the precipitate, but also in better recovery of conglycinin in the supernatant. After a two-dimensional SDS-PAGE analysis (the first dimension, minus 2-ME; the second, plus 2-ME) of the precipitates, superimposition between the CBB-stained gel and the eletroblotted membrane stained with a monoclonal antibody specific to Gly m Bd 30 K indicated that part of Gly m Bd 30 K was preferentially bound to the alpha'- and alpha-subunits of conglycinin, and that part of them had formed the dimer through a disulfide bond.
Samoto
M
M
Central Research Institute, Fuji Oil Co., Ltd., Ibaraki, Japan.
Miyazaki
C
C
Akasaka
T
T
Mori
H
H
Kawamura
Y
Y
D000485:Allergens / Q000378:metabolism*; D052179:Antigens, Plant; D005916:Globulins / Q000378:metabolism*; D015151:Immunoblotting; D008623:Mercaptoethanol / Q000494:pharmacology; D010940:Plant Proteins / Q000378:metabolism*; D011485:Protein Binding; D055314:Seed Storage Proteins; D030262:Soybean Proteins; D013025:Glycine max / Q000378:metabolism*
D000485:Allergens; D052179:Antigens, Plant; D005916:Globulins; D010940:Plant Proteins; D055314:Seed Storage Proteins; D030262:Soybean Proteins; C100565:alpha-conglycinin, Glycine max; D008623:Mercaptoethanol