Coincidental expression of E5a and c-jun in human papillomavirus type 6/11-infected condylomata.
Previously, we have shown that E5a can induce expression of the c-jun gene in human papillomavirus (HPV)-11 E5a transformed NIH 3T3 cells and human epidermal keratinocytes. In this study, we investigated the relationship between expression of the E5a gene and c-jun in pathologically confirmed condylomata specimens using mRNA hybridization in situ. The c-jun RNA concentration was significantly higher in condylomata specimens with E5a mRNA expression than in specimens without E5a mRNA expression, or in normal cervical specimens. The cells with c-jun expression were located predominantly in the basal and parabasal cell layers. These layers were also the primary location of E5a-expressing cells. This is the first demonstration of a strong correlation (74%) between expression of the E5a and c-jun genes in condylomata specimens. This correlation might reflect regulation by HPV-11 E5a of c-jun gene expression in condyloma.
Chen
S L
SL
Department of Microbiology & Immunology, National Defense Medical Center, Taipei, Taiwan, Republic of China. slchen@ndmci.ndmctsgh.edu.tw
Hsieh
T B
TB
Tsao
Y P
YP
Han
C P
CP
Yang
Y F
YF
D016475:3T3 Cells; D000818:Animals; D002478:Cells, Cultured; D003218:Condylomata Acuminata / Q000235:genetics* / Q000473:pathology / Q000821:virology*; D005260:Female; D016758:Genes, jun; D006801:Humans; D017403:In Situ Hybridization; D015603:Keratinocytes / Q000166:cytology; D008297:Male; D051379:Mice; D009856:Oncogene Proteins, Viral / Q000096:biosynthesis*; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D016755:Proto-Oncogene Proteins c-jun / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000096:biosynthesis; D012189:Retrospective Studies; D012867:Skin / Q000166:cytology; D014158:Transcription, Genetic
C081917:E5A protein, Human papillomavirus type 11; D009856:Oncogene Proteins, Viral; D016755:Proto-Oncogene Proteins c-jun; D012333:RNA, Messenger