Mechanisms and uses of hydrogen exchange.
Recent work has largely completed our understanding of the hydrogen-exchange chemistry of unstructured proteins and nucleic acids. Some of the high-energy structural fluctuations that determine the hydrogen-exchange behavior of native macromolecules have been explained; others remain elusive. A growing number of applications are exploiting hydrogen-exchange behavior to study difficult molecular systems and elicit otherwise inaccessible information on protein structure, dynamics and energetics.
Englander
S W
SW
Johnson Research Foundation, Department of Biochemistry & Biophysics, University of Pennsylvania School of Medicine, Philadelphia 19104-6059, USA. walter@hx2.med.upenn.edu
Sosnick
T R
TR
Englander
J J
JJ
Mayne
L
L
D006801:Humans; D006859:Hydrogen / Q000378:metabolism*; D011487:Protein Conformation; D017510:Protein Folding; D011506:Proteins / Q000378:metabolism*; D011522:Protons
D011506:Proteins; D011522:Protons; D006859:Hydrogen