title: Efficacy of perfluorocarbon partial liquid ventilation in a large animal model of acute respiratory failure.
abstract: OBJECTIVE
  To demonstrate the efficacy of partial perfluorocarbon liquid ventilation in large animal model of acute respiratory failure.
  
  
  METHODS
  Prospective, randomized, controlled trial.
  
  
  METHODS
  Animal laboratory at a university medical center.
  
  
  METHODS
  Ten adult sheep, weighing 53.0 +/- 2.8 kg.
  
  
  METHODS
  After assessment of baseline physiologic data, acute respiratory failure was induced by right atrial injection of oleic acid (0.2 mL/kg). Five animals (partial liquid ventilation group) underwent sequential intratracheal dosing of 10 mL/kg of perflubron at 30-min intervals to the following cumulative doses: 10, 20, 30, 40, and 50 mL/kg. The remaining five animals were gas ventilated (control group). Physiologic data were assessed at 30-min intervals in both groups for the 2.5-hr experimental period or until death.
  
  
  RESULTS
  When compared with control animals, intratracheal perfluorocarbon instillation resulted in significant improvements in arterial oxygen saturation (arterial oxygen saturation after 50 mL/kg: partial liquid ventilation, 96 +/- 3%; control, 55 +/- 8%; p = .001) and physiologic shunt (physiologic shunt after 50 mL/kg dose: partial liquid ventilation, 2 +/- 8%; control, 64 +/- 5%; p = .004). Oxygen delivery improved with perfluorocarbon instillation, but this improvement was not significant. No significant difference in pulmonary compliance was observed during partial liquid ventilation when compared with controls (pulmonary compliance: partial liquid ventilation, 0.43 +/- 0.04 mL/ cm H2O/kg; control, 0.53 +/- 0.03 mL/cm H2O/kg; p = .102).
  
  
  CONCLUSIONS
  Partial liquid ventilation with perflubron provides effective improvement in gas exchange in an adult animal model of respiratory failure.
authors.affiliation: Department of Surgery, University of Michigan, Ann Arbor 48109-0245, USA.
authors.forename: M C
authors.initials: MC
authors.lastname: Overbeck
authors.forename: T
authors.initials: T
authors.lastname: Pranikoff
authors.forename: C M
authors.initials: CM
authors.lastname: Yadao
authors.forename: R B
authors.initials: RB
authors.lastname: Hirschl
mesh_terms: D000208:Acute Disease; D000280:Administration, Inhalation; D000818:Animals; D004195:Disease Models, Animal; D005466:Fluorocarbons / Q000008:administration & dosage*; D011446:Prospective Studies; D011897:Random Allocation; D012121:Respiration, Artificial; D012128:Respiratory Distress Syndrome / Q000188:drug therapy; D012131:Respiratory Insufficiency / Q000188:drug therapy*; D012756:Sheep
chemical_list: D005466:Fluorocarbons