title: Epidermal growth factor promotes rapid response to epithelial injury in rabbit duodenum in vitro.
abstract: OBJECTIVE
  Growth factors are mainly involved in the regulation of intestinal epithelial barrier function. This study investigated the effect of epidermal growth factor (EGF) and insulin-like growth factor 1 (IGF-1) on epithelial restitution of rabbit duodenum in vitro.
  
  
  METHODS
  Rabbit duodenal mucosal strips mounted in an Ussing chamber were luminally exposed to 10 mmol/L HCl for 10 minutes and then incubated with buffer alone or luminal buffer containing various concentrations of EGF and IGF-1 for 3 hours. Resistance was calculated from potential difference and short-circuit current. Damage was assessed by morphometry on H&E-stained sections.
  
  
  RESULTS
  HCl caused resistance to decrease from 112 +/- 2 to 51 +/- 4 ohms x cm2 10 minutes after injury (n = 6; P < 0.05). Postinjury treatment with 25 or 50 ng/mL luminal EGF for 3 hours stimulated resistance to recover to 94 +/- 3 and 104 +/- 3 ohms x cm2, respectively, vs. 81 +/- 3 omega x cm2 in controls (P < 0.05). Ten minutes after injury, 62% of the mucosa was damaged; 3 hours after injury, damage was reduced to 24% +/- 1.09% and 10% +/- 1.42% in the 25 and 50 ng/mL EGF group, respectively, vs. 38% +/- 0.93% in controls (n = 6 per group). EGF stimulated enterocyte migration. IGF-1 did not impair epithelial restitution.
  
  
  CONCLUSIONS
  EGF, but not IGF-1, promoted epithelial restitution of rabbit duodenum in vitro.
authors.affiliation: University Clinic of Surgery, Vienna; Austria.
authors.forename: M
authors.initials: M
authors.lastname: Riegler
authors.forename: R
authors.initials: R
authors.lastname: Sedivy
authors.forename: T
authors.initials: T
authors.lastname: Sogukoglu
authors.forename: E
authors.initials: E
authors.lastname: Cosentini
authors.forename: G
authors.initials: G
authors.lastname: Bischof
authors.forename: B
authors.initials: B
authors.lastname: Teleky
authors.forename: W
authors.initials: W
authors.lastname: Feil
authors.forename: R
authors.initials: R
authors.lastname: Schiessel
authors.forename: G
authors.initials: G
authors.lastname: Hamilton
authors.forename: E
authors.initials: E
authors.lastname: Wenzl
mesh_terms: D000818:Animals; D002465:Cell Movement; D004386:Duodenum / Q000473:pathology* / Q000502:physiology; D004594:Electrophysiology; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D004848:Epithelium / Q000473:pathology / Q000502:physiology; D066298:In Vitro Techniques; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology; D007413:Intestinal Mucosa / Q000473:pathology / Q000502:physiology; D011817:Rabbits
chemical_list: D004815:Epidermal Growth Factor; D007334:Insulin-Like Growth Factor I