[An artificial gene, biosynthesis and properties of the recombinant Fc fragment of human immunoglobulin G1].
Chemicoenzymatic synthesis and cloning of a gene encoding the Fc domain of human immunoglobulin G1 were carried out. The artificial gene was expressed in Escherichia coli cells in plasmid vectors under control of a late T7 promoter. The recombinant protein isolated from the bacterial cells is capable of forming dimers and binding protein A from Staphylococcus aureus.
Efimov
V A
VA
Kalinkina
A L
AL
Fradkov
A F
AF
Chakhmakhcheva
O G
OG
D000595:Amino Acid Sequence; D017123:Bacteriophage T7 / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular; D004274:DNA, Recombinant; D005813:Genes, Synthetic; D006801:Humans; D007141:Immunoglobulin Fc Fragments / Q000235:genetics*; D007074:Immunoglobulin G / Q000235:genetics*; D008969:Molecular Sequence Data; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000235:genetics
D004274:DNA, Recombinant; D007141:Immunoglobulin Fc Fragments; D007074:Immunoglobulin G; D011994:Recombinant Proteins
Iskusstvennyĭ gen, biosintez i svoĭstva rekombinantnogo Fc-fragmenta immunoglobulina G1 cheloveka.