title: Mechanisms and uses of hydrogen exchange.
abstract: Recent work has largely completed our understanding of the hydrogen-exchange chemistry of unstructured proteins and nucleic acids. Some of the high-energy structural fluctuations that determine the hydrogen-exchange behavior of native macromolecules have been explained; others remain elusive. A growing number of applications are exploiting hydrogen-exchange behavior to study difficult molecular systems and elicit otherwise inaccessible information on protein structure, dynamics and energetics.
authors.affiliation: Johnson Research Foundation, Department of Biochemistry & Biophysics, University of Pennsylvania School of Medicine, Philadelphia 19104-6059, USA. walter@hx2.med.upenn.edu
authors.forename: S W
authors.initials: SW
authors.lastname: Englander
authors.forename: T R
authors.initials: TR
authors.lastname: Sosnick
authors.forename: J J
authors.initials: JJ
authors.lastname: Englander
authors.forename: L
authors.initials: L
authors.lastname: Mayne
mesh_terms: D006801:Humans; D006859:Hydrogen / Q000378:metabolism*; D011487:Protein Conformation; D017510:Protein Folding; D011506:Proteins / Q000378:metabolism*; D011522:Protons
chemical_list: D011506:Proteins; D011522:Protons; D006859:Hydrogen