title: Muscle protein synthesis rate decreases 24 hours after abdominal surgery irrespective of total parenteral nutrition.
abstract: BACKGROUND
  Muscle protein synthesis rate is known to decrease postoperatively as a part of the catabolic response to trauma. Conventional total parenteral nutrition (TPN) in the postoperative period does not seem to counteract the decrease in protein synthesis. However, it is still unclear if ongoing TPN given continuously after surgery would inhibit this fall in muscle protein synthesis.
  
  
  METHODS
  The rate of protein synthesis in skeletal muscle was determined before and 24 hours after open cholecystectomy, used as a standardized human model of trauma. Patients (n = 14) were randomized to receive either TPN continuously throughout the postoperative period or saline as postoperative fluid therapy. The protein synthesis rate was calculated from the increase in enrichment of labeled phenylalanine in protein after an IV flooding dose of [2H5] phenylalanine, 45 mg/kg body weight.
  
  
  RESULTS
  The fractional synthesis rate decreased by 31% from 1.74 +/- 0.13% to 1.15 +/- 0.10% per 24 hours in the saline group (p < .02) and by 23% from 1.59 +/- 0.10% to 1.22 +/- 0.07% per 24 hours in the group receiving TPN (p < .01), showing no significant difference between the two groups.
  
  
  CONCLUSIONS
  A continuous and ongoing infusion of conventional TPN started immediately after surgery did not counteract the obligatory decline of muscle protein synthesis, observed 24 hours postoperatively.
authors.affiliation: Department of Anesthesiology, Huddinge University Hospital, Karolinska Institute, Stockholm, Sweden.
authors.forename: I
authors.initials: I
authors.lastname: Tjäder
authors.forename: P
authors.initials: P
authors.lastname: Essen
authors.forename: A
authors.initials: A
authors.lastname: Thörne
authors.forename: P J
authors.initials: PJ
authors.lastname: Garlick
authors.forename: J
authors.initials: J
authors.lastname: Wernerman
authors.forename: M A
authors.initials: MA
authors.lastname: McNurlan
mesh_terms: D000328:Adult; D001786:Blood Glucose / Q000378:metabolism; D002763:Cholecystectomy; D005260:Female; D006801:Humans; D007328:Insulin / Q000097:blood; D007700:Kinetics; D008297:Male; D008875:Middle Aged; D009124:Muscle Proteins / Q000096:biosynthesis*; D018482:Muscle, Skeletal / Q000378:metabolism*; D010289:Parenteral Nutrition, Total
chemical_list: D001786:Blood Glucose; D007328:Insulin; D009124:Muscle Proteins