title: Effect of hypoxia on lung, heart, and liver insulin-like growth factor-I gene and receptor expression in the newborn rat.
abstract: OBJECTIVE
  We examined the effect of 7 days of hypoxia in the newborn rat on: a) body, heart, and lung growth; b) circulating insulin-like growth factor-I (IGF-I); c) lung, heart, and liver IGF-I gene expression; and d) lung IGF-I type 1 receptor gene expression and IGF-I receptor binding. We hypothesize that hypoxic exposure would modify body and organ growth and alter IGF-I gene and receptor expression in an organ specific manner.
  
  
  METHODS
  Randomized, controlled prospective study.
  
  
  METHODS
  University research laboratory.
  
  
  METHODS
  Eleven newborn rat litters (n = 10 per litter) comprised the hypoxia-exposed group and 11 litters comprised the control group (room air).
  
  
  METHODS
  Hypoxia-group rats were placed in a chamber with an FIO2 of 0.12 on postnatal day 1. Control group rats breathed room air. Exposure to hypoxia continued for 7 days.
  
  
  RESULTS
  Hepatic, lung, and cardiac IGF-I mRNA levels and lung IGF-I type 1 receptor mRNA were analyzed, using the ribonuclease protection assay. Crude membrane extracts were used for competitive binding studies with IGF-I and insulin. Somatic growth in the hypoxic group was reduced by 22% (final weight: hypoxic, 14.8 +/- 1.2 g; control, 17.1 +/- 1.5 g; p < .001). The relative weight (organ weight/body weight [mg/g]) of the heart was increased by 39% (p < .001) in the hypoxic pups compared with the normoxic animals, while the relative weight of the lung was unchanged. With hypoxia, IFG-I mRNA concentrations were significantly increased both in the heart and lung (30% and 33%, respectively, p < .02); but, in contrast, IGF-I mRNA concentrations were not significantly different in the liver. The IGF-I receptor mRNA in the lung was increased by 200% (p < .02) in hypoxia compared with controls. There was no effect of hypoxia on specific or nonspecific binding of IGF-I or insulin in the lung tissue. However, specific binding was 33% greater in the IGF-I compared with the insulin experiments.
  
  
  CONCLUSIONS
  a) Hypoxia increased IGF-I mRNA in the heart, and increased both IGF-I mRNA and IGF-I type 1 receptor mRNA in the lung. b) The effects of hypoxia on IFG-I are tissue-specific.
authors.affiliation: Division of Respiratory and Critical Care, Harbor-UCLA Medical Center, Torrance, CA 90509, USA.
authors.forename: D Y
authors.initials: DY
authors.lastname: Moromisato
authors.forename: M Y
authors.initials: MY
authors.lastname: Moromisato
authors.forename: S
authors.initials: S
authors.lastname: Zanconato
authors.forename: C T
authors.initials: CT
authors.lastname: Roberts
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001667:Binding, Competitive; D001835:Body Weight; D004435:Eating; D005260:Female; D018507:Gene Expression Regulation, Developmental; D006321:Heart / Q000254:growth & development*; D000860:Hypoxia / Q000503:physiopathology*; D007334:Insulin-Like Growth Factor I / Q000235:genetics* / Q000378:metabolism; D008099:Liver / Q000254:growth & development*; D008168:Lung / Q000254:growth & development*; D009929:Organ Size; D011446:Prospective Studies; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D017526:Receptor, IGF Type 1 / Q000235:genetics / Q000378:metabolism
chemical_list: D007334:Insulin-Like Growth Factor I; D017526:Receptor, IGF Type 1