Preparation of liposomes that mimic the membrane of endoplasmic reticulum of rat hepatocytes.
To examine the interaction between biomembranes and membrane-bound proteins, large unilamellar liposomes have been required. In the present study, we prepared liposomes from a mixture of phospholipids having a phospholipid composition similar to that in the endoplasmic reticula (microsomes) of rat hepatocytes by eight different methods. The resulting liposomes were examined by a combination of the freeze-fracture-replica procedure with biochemical methods. The freeze-thawing method of Pick (1981) gave the best results; large unilamellar liposomes that mimic the membrane of endoplasmic reticulum were obtained. Liposomes made by this method are thus suitable for analysis of the interaction between the endoplasmic reticulum membrane and membrane-bound proteins.
Watanabe
J
J
Department of Anatomy, Kansai Medical University, Osaka, Japan.
Asaka
Y
Y
Mino
K
K
Kanamura
S
S
D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000378:metabolism / Q000648:ultrastructure*; D005614:Freeze Fracturing / Q000379:methods*; D007425:Intracellular Membranes / Q000378:metabolism / Q000648:ultrastructure; D008081:Liposomes / Q000737:chemistry / Q000302:isolation & purification; D008099:Liver / Q000378:metabolism / Q000648:ultrastructure*; D008854:Microscopy, Electron / Q000379:methods; D009251:NADPH-Ferrihemoprotein Reductase / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
D008081:Liposomes; D003577:Cytochrome P-450 Enzyme System; D009251:NADPH-Ferrihemoprotein Reductase