Immunohistochemical determination of glutathione S-transferases in gastric carcinomas and in adjacent normal gastric epithelium.
Glutathione S-transferases (GSTs) are a family of isoenzymes that play an important role in protecting cells against cytotoxic and carcinogenic agents. The distribution and levels of GST Alpha and Pi in normal and malignant gastric tissue of 34 patients with gastric cancer were examined immunohistochemically. Expression of GST Alpha and Pi was observed in 47 and 100 percent of the tumors, respectively. In normal mucosa both enzyme classes were present in 100 percent of the specimens. Mucous cells showed staining for GST Alpha and Pi in 88 and 97 percent, parietal cells in 93 and 67 percent, and chief cells in 82 and 30 percent, respectively. No correlation was observed between the amount or pattern of GST Alpha or Pi in carcinomas and the clinical and pathological characteristics of the patients. So it can be concluded that both GST Alpha and Pi cannot be considered as prognostic factors for gastric cancer.
Schipper
D L
DL
Department of Medical Oncology, University Hospital Nijmegen, The Netherlands.
Wagenmans
M J
MJ
Van Haelst
U
U
Peters
W H
WH
Wobbes
T
T
Verhofstad
A A
AA
Lange
W P
WP
Wagener
D J
DJ
D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000378:metabolism*; D005260:Female; D005753:Gastric Mucosa / Q000201:enzymology*; D005982:Glutathione Transferase / Q000032:analysis*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000378:metabolism*; D013274:Stomach Neoplasms / Q000201:enzymology*
D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins; D005982:Glutathione Transferase