title: Association of gastric adenocarcinoma with the HLA class II gene DQB10301.
abstract: OBJECTIVE
  The HLA class II gene DQB1*0301 has been linked to several cancers. This study was designed to determine if HLA-DQB1*0301 is present at altered frequency in patients with gastric, colorectal, or pancreatic adenocarcinoma.
  
  
  METHODS
  Oligotyping for HLA-DQB1*0301 was performed for 159 Caucasian patients with 160 gastrointestinal adenocarcinomas (52 gastric, 62 colorectal, and 46 pancreatic adenocarcinomas) and compared with 260 Caucasian noncancer controls. Patients with gastric adenocarcinoma underwent extended HLA class II region oligotyping. Immunoglobulin G to Helicobacter pylori was detected by enzyme-linked immunosorbent assay.
  
  
  RESULTS
  HLA-DQB1*0301 was more common in patients with gastric adenocarcinoma than controls (54% vs. 27%; bonferroni-corrected chi 2 P = 0.003; odds ratio, 3.2). HLA-DQB1*0301 was not associated with colorectal or pancreatic adenocarcinoma. No other HLA-DQB1 allele and no HLA-DQA1 or transporter associated with antigen processing 2 (TAP2) allele were present at altered frequency in patients with gastric adenocarcinoma. Serological evidence for H. pylori infection was less frequent in HLA-DQB1*0301-positive patients with gastric adenocarcinoma compared with HLA-DQB1*0301-negative patients (52% vs. 88%; Fisher's Exact Test; P = 0.007).
  
  
  CONCLUSIONS
  HLA-DQB1*0301 is more common in caucasian patients with gastric adenocarcinoma than noncancer controls. The mechanism linking HLA-DQB1*0301 with gastric adenocarcinoma is not likely through increased susceptibility to H. pylori infection.
authors.affiliation: Department of Surgical Oncology, University of Texas M. D. Anderson Cancer Center, Houston, USA.
authors.forename: J E
authors.initials: JE
authors.lastname: Lee
authors.forename: A M
authors.initials: AM
authors.lastname: Lowy
authors.forename: W A
authors.initials: WA
authors.lastname: Thompson
authors.forename: M
authors.initials: M
authors.lastname: Lu
authors.forename: P T
authors.initials: PT
authors.lastname: Loflin
authors.forename: J M
authors.initials: JM
authors.lastname: Skibber
authors.forename: D B
authors.initials: DB
authors.lastname: Evans
authors.forename: S A
authors.initials: SA
authors.lastname: Curley
authors.forename: P F
authors.initials: PF
authors.lastname: Mansfield
authors.forename: J D
authors.initials: JD
authors.lastname: Reveille
mesh_terms: D000071450:ATP Binding Cassette Transporter, Subfamily B, Member 3; D018528:ATP-Binding Cassette Transporters / Q000235:genetics; D000230:Adenocarcinoma / Q000235:genetics / Q000276:immunology*; D016009:Chi-Square Distribution; D015179:Colorectal Neoplasms / Q000235:genetics / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005787:Gene Frequency; D005802:Genes, MHC Class II; D006683:HLA-DQ Antigens / Q000235:genetics*; D059848:HLA-DQ alpha-Chains; D059866:HLA-DQ beta-Chains; D016480:Helicobacter pylori / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D010190:Pancreatic Neoplasms / Q000235:genetics / Q000276:immunology; D013274:Stomach Neoplasms / Q000235:genetics / Q000276:immunology*
chemical_list: D000071450:ATP Binding Cassette Transporter, Subfamily B, Member 3; D018528:ATP-Binding Cassette Transporters; D006683:HLA-DQ Antigens; D059848:HLA-DQ alpha-Chains; D059866:HLA-DQ beta-Chains; C069050:HLA-DQA1 antigen; C069051:HLA-DQB1 antigen; D007074:Immunoglobulin G; C071692:TAP2 protein, human