Determination of serotonin released from coffee wax by liquid chromatography.
A simple hydrolysis and extraction method was developed for the release of serotonin (5-hydroxytryptamine) from a coffee wax sample obtained from decaffeination of coffee beans. The recoverable amount of serotonin was determined by reversed-phase high-performance liquid chromatography with gradient elution and UV detection, using the standard addition method. Different type of basic deactivated chromatographic columns were used for the separation.
Kele
M
M
Department of Medical Chemistry, University Medical School, Pécs, Hungary.
Ohmacht
R
R
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003069:Coffee / Q000737:chemistry*; D006868:Hydrolysis; D019800:Phenol; D010636:Phenols / Q000302:isolation & purification; D011725:Pyridines / Q000302:isolation & purification; D012701:Serotonin / Q000032:analysis*; D014885:Waxes
D003069:Coffee; D010636:Phenols; D011725:Pyridines; D014885:Waxes; D012701:Serotonin; D019800:Phenol; C023666:pyridine