Iron metabolism-related genes and mitochondrial genes are induced during involution of mouse mammary gland.
To understand molecular mechanisms of mammary gland involution, several clones were isolated after the primary differential screening of a total 40,000 pfu of involution-specific cDNA library, and further characterized. The partial sequences and Northern analysis revealed that iron metabolism-related genes and mitochondrial genes were induced during mammary gland involution. The expression of the lactoferrin gene was induced at involution days 1, 2, and 3. The expression of ferritin heavy chain gene was induced at involution days 1, 2, 3 and 4. Cytochrome oxidase subunit 1 and cytochrome oxidase subunit 2 genes were induced at involution days 4 and 7. The expression of cytochrome b gene was induced at involution day 7. These results imply that iron metabolism and mitochondrial function may be altered during mammary gland involution.
Lee
M
M
Department of Genetic Engineering, Chonnam National University, Kwangju, Korea.
Kim
H
H
Jeon
D
D
Hwang
I
I
Choi
B
B
Myung
K
K
Kim
H
H
Choi
Y
Y
Paik
S
S
Baik
M
M
D000818:Animals; D015152:Blotting, Northern; D015139:Blotting, Southern; D003001:Cloning, Molecular; D003573:Cytochrome b Group / Q000096:biosynthesis*; D018076:DNA, Complementary; D003576:Electron Transport Complex IV / Q000096:biosynthesis*; D004926:Escherichia coli; D005260:Female; D005786:Gene Expression Regulation; D015723:Gene Library; D007501:Iron / Q000378:metabolism*; D007781:Lactoferrin / Q000096:biosynthesis*; D008321:Mammary Glands, Animal / Q000502:physiology*; D051379:Mice; D008813:Mice, Inbred ICR; D008928:Mitochondria / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D014158:Transcription, Genetic; D014886:Weaning
D003573:Cytochrome b Group; D018076:DNA, Complementary; D007501:Iron; D003576:Electron Transport Complex IV; D007781:Lactoferrin