title: Short report: simultaneous detection by polymerase chain reaction of mosquito species and Plasmodium falciparum infection in Anopheles gambiae sensu lato.
abstract: Total RNA purified from Anopheles gambiae mosquitoes can be used for detection of both 1) infection by Plasmodium falciparum using a reverse transcriptase-polymerase chain reaction (RT-PCR) assay specific for P. falciparum ribosomal RNA (rRNA) of sporogonic stage parasites, and 2) mosquito species using a PCR assay that distinguishes members of the Anopheles gambiae complex.
authors.affiliation: Departement d'Epidemiologie des Affectations Parasitaires, Ecole Nationale de Medecine et de Pharmacie, Bamako, Mali.
authors.forename: M
authors.initials: M
authors.lastname: Bouare
authors.forename: D
authors.initials: D
authors.lastname: Sangare
authors.forename: M
authors.initials: M
authors.lastname: Bagayoko
authors.forename: A
authors.initials: A
authors.lastname: Toure
authors.forename: Y T
authors.initials: YT
authors.lastname: Toure
authors.forename: K D
authors.initials: KD
authors.lastname: Vernick
mesh_terms: D000818:Animals; D000852:Anopheles / Q000145:classification* / Q000469:parasitology; D007303:Insect Vectors / Q000145:classification* / Q000469:parasitology; D010963:Plasmodium falciparum / Q000254:growth & development / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D016053:RNA, Protozoan / Q000032:analysis*; D012335:RNA, Ribosomal / Q000032:analysis*; D013045:Species Specificity
chemical_list: D016053:RNA, Protozoan; D012335:RNA, Ribosomal