Intranasal monoclonal IgA antibody to respiratory syncytial virus protects rhesus monkeys against upper and lower respiratory tract infection.
Respiratory syncytial virus (RSV), the major cause of lower respiratory tract disease in infants, is thought to infect the upper airways before spreading to the lower respiratory tract. A rhesus monkey model of RSV infection after upper airway inoculation was used to test the protective effect of intranasal treatment with HNK20, a mouse monoclonal IgA antibody against RSV F glycoprotein. HNK20 was administered once daily for 2 days before RSV challenge and 4 days after challenge. Treatment with 0.5 mg/kg HNK20 reduced viral shedding in the nose, throat, and lungs by 3-4 log10/mL (P < or = .002). All monkeys developed RSV neutralizing antibody in serum, even in the absence of detectable viral replication. Neutralizing concentrations of monoclonal antibody remained in nasal secretions for > 1 day after treatment. These results suggest that nose-drop application of monoclonal antibody could provide convenient and effective protection against RSV infection in human infants at risk of severe lower respiratory tract disease.
Weltzin
R
R
OraVax, Inc., Cambridge, Massachusetts 02139, USA.
Traina-Dorge
V
V
Soike
K
K
Zhang
J Y
JY
Mack
P
P
Soman
G
G
Drabik
G
G
Monath
T P
TP
D000281:Administration, Intranasal; D000818:Animals; D000911:Antibodies, Monoclonal / Q000627:therapeutic use*; D000914:Antibodies, Viral / Q000627:therapeutic use*; D001992:Bronchoalveolar Lavage Fluid / Q000821:virology; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D015707:HN Protein; D007071:Immunoglobulin A, Secretory / Q000627:therapeutic use*; D008253:Macaca mulatta; D051379:Mice; D009297:Nasal Mucosa / Q000276:immunology; D010614:Pharynx / Q000821:virology; D018357:Respiratory Syncytial Virus Infections / Q000517:prevention & control*; D012136:Respiratory Syncytial Viruses / Q000276:immunology / Q000302:isolation & purification; D012141:Respiratory Tract Infections / Q000517:prevention & control*; D013997:Time Factors; D014759:Viral Envelope Proteins; D014764:Viral Proteins / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D015707:HN Protein; D007071:Immunoglobulin A, Secretory; D014759:Viral Envelope Proteins; D014764:Viral Proteins; C116204:attachment protein G