title: Interaction of lysozyme with a surface protein antigen of Streptococcus mutans.
abstract: The interaction of salivary lysozyme with the surface protein antigen (PAc) of Streptococcus mutans and the interaction of lysozyme with the pathogen were examined by ELISA using S. mutans MT8148 (PAc+) and the PAc-defective mutant EM-2 (PAc-). The lysozyme clearly bound to the S. mutans wild type but not to the S. mutans mutant. Furthermore, lysozyme bound directly in the fluid phase to the rPAc, of which the binding kinetics were determined (Kon = 3.63 +/- 0.04 x 10(3) M-1 s-1, K(off) = 1.72 +/- 0.04 x 10-5 s-1 and Kon/K(off) = 2.11 x 10(8) M-1) using surface plasmon resonance. The kinetics of both association and dissociation were relatively slow. In addition, anti-lysozyme antibody significantly inhibited the binding of salivary components to the rPAc. The present findings indicate that lysozyme is one of the major salivary components interacting with S. mutans PAc.
authors.affiliation: Department of Oral Science, National Institute of Health, Tokyo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Senpuku
authors.forename: H
authors.initials: H
authors.lastname: Kato
authors.forename: M
authors.initials: M
authors.lastname: Todoroki
authors.forename: N
authors.initials: N
authors.lastname: Hanada
authors.forename: T
authors.initials: T
authors.lastname: Nisizawa
mesh_terms: D000328:Adult; D000954:Antigens, Surface / Q000378:metabolism; D001422:Bacterial Adhesion / Q000502:physiology; D001426:Bacterial Proteins / Q000378:metabolism; D015374:Biosensing Techniques; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007700:Kinetics; D008297:Male; D009113:Muramidase / Q000037:antagonists & inhibitors / Q000378:metabolism* / Q000494:pharmacology; D009154:Mutation / Q000502:physiology; D011485:Protein Binding / Q000502:physiology; D012463:Saliva / Q000201:enzymology; D013295:Streptococcus mutans / Q000276:immunology*
chemical_list: D000954:Antigens, Surface; D001426:Bacterial Proteins; D009113:Muramidase