[Study on the specific killing activity of albumin nanoparticles containing adriamycin targeted by monoclonal antibody BDI-1 to human bladder cancer cells].
A highly specific immuno-nanoparticle (ADR-NP-Ab) has been constituted by chemically coupling a monoclonal antibody BDI-1 to albumin nanoparticle containing adriamycin (ADR-NP). Different molecular ratios of antibody to ADR-NP were tried to determine the optimal condition for preparing the immuno-nanoparticle. The results of immunoflurecence and microphotographic analysis showed that the activity of ADR-NP-Ab was well preserved. The result of the cytotoxicity of ADR-NP-Ab in vitro assay showed strong killing activity of ADR-NP-Ab to bladder cancer cells (EJ), while no apparent cytotoxic activity to non-targeted human colon carcinoma cells (Lovo) was observed.
Sheng
J
J
Department of Pharmaceutics, Beijing Medical University.
Samten
B K
BK
Xie
S S
SS
Wei
S L
SL
D000418:Albumins; D000818:Animals; D000911:Antibodies, Monoclonal; D004317:Doxorubicin / Q000494:pharmacology*; D005260:Female; D006168:Guinea Pigs; D000922:Immunotoxins / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008863:Microspheres; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000276:immunology; D001749:Urinary Bladder Neoplasms / Q000473:pathology*
D000418:Albumins; D000911:Antibodies, Monoclonal; D000922:Immunotoxins; D004317:Doxorubicin