[Secretion of endothelin-1 by trophoblast cells and binding of endothelin-1 to trophoblast membranes in healthy and pre-eclampsia pregnancies].
OBJECTIVE
The hypothesis was tested that the synthesis or binding of endothelin-1 (ET-1) by the trophoblast are altered in preeclampsia.


METHODS
Trophoblast cells (TC) were isolated from 15 normal (C) und 18 pre-eclamptic pregnancies (P). Binding of [125I]ET-1 to plasma membranes of TC was determined after 48 h in culture. Concentration of ET-1 in culture media were measured after each 24 h over 5 day culture period.


RESULTS
Specific binding was 150% (week 37-40) - 400% (week 31-36) higher (P < 0.01) in P. In contrast, the release of ET-1 was 60% (week 31-36) - 71% (week 37-40) lower (P < 0.01) in P than in healthy controls.


CONCLUSIONS
Pre-eclampsia is associated with a lower release and increased binding of ET-1 by trophoblast cells.
Cervar
M
M
Geburtshilflich-Gynäkologische Universitätsklinik Graz.
Kainer
F
F
Desoye
G
G
D000328:Adult; D002462:Cell Membrane / Q000473:pathology; D016232:Endothelins / Q000378:metabolism*; D005260:Female; D005865:Gestational Age; D006801:Humans; D007231:Infant, Newborn; D011225:Pre-Eclampsia / Q000473:pathology*; D011247:Pregnancy; D011869:Radioligand Assay; D044022:Receptor, Endothelin A; D017466:Receptors, Endothelin / Q000378:metabolism*; D012016:Reference Values; D014327:Trophoblasts / Q000473:pathology*
D016232:Endothelins; D044022:Receptor, Endothelin A; D017466:Receptors, Endothelin
Sekretion von Endothelin-1 aus Trophoblastzellen und Bindung von Endothelin-1 an Trophoblastmembranen bei gesunden und präeklamptischen Schwangerschaften.