Experimental liver fibrosis induced in rats receiving high doses of alcohol and alternating between regular and vitamin-depleted diets.
Liver fibrosis was induced in rats by simulating human alcoholic eating and drinking patterns. Alcohol addiction was established by gradually increasing the ethanol concentration in the drinking water; salts were added at the terminal stage. The hepatocytes of rats receiving alcohol concentrations exceeding 50% (v/v) (similar to vodka) exhibited alcoholic hyaline (Mallory bodies). Alcoholic liver fibrosis was induced by alternating between regular and autoclaved (vitamin-depleted) diets, simulating the irregular eating habits of human alcoholics. In the livers of rats receiving 70% (v/v) ethanol (comparable to absinthe) with 25% saline and fed the alternating diets, pericellular fibrosis was induced. No significant difference in calorie intake between control and alcohol rats was detected except when rats underwent drinking bouts (heavy drinking phase). This indicates that neither a high-fat diet nor a choline-depleted diet is necessary to induce the alcoholic fibrosis seen in human alcoholics.
Hirano
H
H
Department of Biochemistry, University of Occupational and Environmental Health, Kitakyushu, Japan.
Hirano
T
T
Hirata
K
K
Tamura
M
M
Yamaura
T
T
Hamada
T
T
D000818:Animals; D001361:Avitaminosis / Q000150:complications; D004032:Diet; D002149:Energy Intake; D000431:Ethanol / Q000008:administration & dosage*; D016326:Extracellular Matrix Proteins / Q000235:genetics; D015870:Gene Expression; D006801:Humans; D008099:Liver / Q000378:metabolism / Q000473:pathology; D008104:Liver Cirrhosis, Alcoholic / Q000209:etiology* / Q000473:pathology; D008106:Liver Cirrhosis, Experimental / Q000209:etiology* / Q000473:pathology; D008297:Male; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D014815:Vitamins / Q000008:administration & dosage*
D016326:Extracellular Matrix Proteins; D012333:RNA, Messenger; D014815:Vitamins; D000431:Ethanol