Comparative mapping on the mouse X chromosome defines a myotubular myopathy equivalent region.
The gene for X-linked myotubular myopathy (MTM1) has been localized to a 300-kb critical region in human Xq28 between IDS and GABRA3. As part of an effort to clone this gene, we developed a YAC contig on the mouse X Chromosome (Chr) which includes loci homologous to those within the human MTM1 critical region. The murine contig consists of 18 YACs and spans 2.5-3.0 Mb. We have aligned the human and murine physical maps by isolating conserved mouse genomic fragments, including CpG islands and trapped exons. We believe that the simultaneous isolation of genes from both mouse and human and continued comparative mapping will prove helpful in the eventual identification of MTM1 and other genes in the region.
de Gouyon
B
B
Department of Molecular and Human Genetics, Baylor College of Medicine, One Baylor Plaza 821T, Houston, TX 77030, USA.
Chatterjee
A
A
Monaco
A
A
Quaderi
N
N
Brown
S D
SD
Herman
G E
GE
D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D018244:Chromosomes, Artificial, Yeast; D003001:Cloning, Molecular; D017124:Conserved Sequence; D017931:DNA Primers; D005819:Genetic Markers; D015698:Genomic Library; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009468:Neuromuscular Diseases / Q000235:genetics*; D016133:Polymerase Chain Reaction; D017027:Protein Tyrosine Phosphatases / Q000235:genetics*; D054558:Protein Tyrosine Phosphatases, Non-Receptor; D013552:Swine; D014960:X Chromosome
D017931:DNA Primers; D005819:Genetic Markers; D017027:Protein Tyrosine Phosphatases; D054558:Protein Tyrosine Phosphatases, Non-Receptor; C099588:myotubularin