A method for evaluating the cariogenicity of oral bacteria using radio-labelled synthetic hydroxyapatite.
Radioactive hydroxyapatite was synthesized using 45Ca to evaluate the cariogenicity of oral streptococci. Discs prepared from it were suspended in media containing sucrose, then inoculated with Streptococcus mutans or Streptococcus sanguis. The radioactivity in the supernatant fluid was measured at specified time intervals. Released 45Ca in the supernatant fluids markedly increased in both species during the experimental period, while Strep. mutans showed much higher decalcification than Strep. sanguis. The present method would be useful for semi-quantitative evaluation of bacterial decalcification ability.
Yamaguchi
R
R
Research Institute of Radioisotope, Asahi University School of Dentistry, Gifu, Japan.
Sato
M
M
Tsuchiya
H
H
Yamamoto
K
K
Doi
Y
Y
Iwaku
F
F
D002132:Calcium Radioisotopes; D003731:Dental Caries / Q000382:microbiology*; D017886:Durapatite / Q000138:chemical synthesis; D013295:Streptococcus mutans / Q000472:pathogenicity*; D013298:Streptococcus sanguis / Q000472:pathogenicity*
D002132:Calcium Radioisotopes; D017886:Durapatite