Expressions of the fibroblast growth factor family (FGF-1, -2 and -4) mRNA in endometrial cancers.
To investigate whether growth, invasion and metastasis of endometrial cancer cells is associated with neovascularization, the expressions of fibroblast growth factor-1 (acidic FGF), -2 (basic FGF) and -4 (hst-1) mRNAs and FGF-2 in endometrial cancers and normal endometria as controls were determined by reverse transcription-polymerase chain reaction-Southern blot and ELISA, respectively, and the relationships between their expressions and histological grades, grades of myometrial invasion or clinical stages of endometrial cancers were analyzed. The levels of FGF-1 mRNA and FGF-2 and its mRNA tended to increase with dedifferentiation (especially grade G3), myometrial invasion (especially grade C) and staging (especially stages III and IV) in endometrial cancers were significantly (p < 0.05) higher than those in normal endometria. However, the levels of FGF-4 mRNA expression were significantly (p < 0.05) lower than those of FGF-1 and -2 mRNAs in both endometrial cancers and normal endometria. Therefore, endometrial cancers might mainly secrete FGF-1 and -2, which leads to neovascularization to provide nutrition, resulting in accelerated growth, invasion and metastasis. Apparently, the increased secretion parallels the progressive malignancy of endometrial cancers.
Fujimoto
J
J
Department of Obstetrics and Gynecology, Gifu University School of Medicine, Japan.
Hori
M
M
Ichigo
S
S
Tamaya
T
T
D000328:Adult; D000368:Aged; D015139:Blotting, Southern; D016889:Endometrial Neoplasms / Q000737:chemistry* / Q000473:pathology / Q000503:physiopathology; D004717:Endometrium / Q000737:chemistry; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000235:genetics; D051519:Fibroblast Growth Factor 4; D005346:Fibroblast Growth Factors / Q000235:genetics*; D006801:Humans; D008875:Middle Aged; D008969:Molecular Sequence Data; D009361:Neoplasm Invasiveness / Q000503:physiopathology; D009362:Neoplasm Metastasis; D009367:Neoplasm Staging; D009389:Neovascularization, Pathologic / Q000503:physiopathology; D016133:Polymerase Chain Reaction; D011518:Proto-Oncogene Proteins / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis*; D012334:RNA, Neoplasm / Q000032:analysis*; D012194:RNA-Directed DNA Polymerase; D012016:Reference Values
C496445:FGF4 protein, human; D051519:Fibroblast Growth Factor 4; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D016222:Fibroblast Growth Factor 2; D005346:Fibroblast Growth Factors; D012194:RNA-Directed DNA Polymerase