title: Immunodetection of apoptosis-regulating proteins in lymphomas from patients with and without human immunodeficiency virus infection.
abstract: The expression of the apoptosis-regulating genes Bcl-2, Bcl-x, Bax, Mcl-1, and p53 analyzed in 4 cases of human immunodeficiency virus (HIV)-associated Hodgkin's disease, in 36 cases of HIV-related non-Hodgkin's lymphomas (NHLs), and in 109 cases of non-HIV-related NHLs by using immunohistochemistry. HIV-associated Hodgkin's disease samples were positive for all markers. For the HIV-related NHL samples, 36, 66, 88, 100, and 94% of the cases were Bcl-2, Bcl-x, Bax, Mcl-1, and p53 were found to be expressed in 69, 65, 82, 83, and 42%, respectively. No significant differences were observed in Bax and Mcl-1 staining between HIV-unrelated NHLs of B cell and T cell types. In contrast, Bcl-2 was positive in 66/79 (83%) and 10/30 (33%) of B cell and T cell HIV-unrelated NHLs, respectively (P2 < 0.001). Peculiar patterns were observed for hairy cell leukemia (Bax+, Bcl-2+, Mcl-1-) and for anaplastic large cell lymphoma (Bax+, Mcl-1+, Bcl-2-) in HIV-unrelated NHLs. Of interest, all cases with a positive expression of Bax were also found to express either Mcl-1 and/or Bcl-2, suggesting that Mcl-1 and Bcl-2 may counteract the pro-apoptosis function of Bax in vivo by protein-protein interaction within the tumor cell, as demonstrated previously in vitro. These results suggest that apoptosis regulation may have a role in the pathogenesis of some HIV-related and HIV-unrelated NHLs.
authors.affiliation: Service d'Hématologie, Centre Hospitalier Universitaire, Hôpital de Purpan, Toulouse, France.
authors.forename: D
authors.initials: D
authors.lastname: Schlaifer
authors.forename: S
authors.initials: S
authors.lastname: Krajewski
authors.forename: S
authors.initials: S
authors.lastname: Galoin
authors.forename: F
authors.initials: F
authors.lastname: Rigal-Huguet
authors.forename: G
authors.initials: G
authors.lastname: Laurent
authors.forename: P
authors.initials: P
authors.lastname: Massip
authors.forename: J
authors.initials: J
authors.lastname: Pris
authors.forename: G
authors.initials: G
authors.lastname: Delsol
authors.forename: J C
authors.initials: JC
authors.lastname: Reed
authors.forename: P
authors.initials: P
authors.lastname: Brousset
mesh_terms: D017209:Apoptosis / Q000502:physiology*; D014408:Biomarkers, Tumor / Q000032:analysis*; D003593:Cytoplasm / Q000473:pathology; D018023:HIV Seronegativity / Q000502:physiology; D006689:Hodgkin Disease / Q000473:pathology* / Q000503:physiopathology; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D016483:Lymphoma, AIDS-Related / Q000473:pathology* / Q000503:physiopathology; D008228:Lymphoma, Non-Hodgkin / Q000473:pathology* / Q000503:physiopathology; D016399:Lymphoma, T-Cell / Q000473:pathology; D009687:Nuclear Proteins / Q000032:analysis*; D011518:Proto-Oncogene Proteins / Q000032:analysis; D016159:Tumor Suppressor Protein p53 / Q000032:analysis
chemical_list: D014408:Biomarkers, Tumor; D009687:Nuclear Proteins; D011518:Proto-Oncogene Proteins; D016159:Tumor Suppressor Protein p53