Three ATP1 genes are present on chromosome II in Saccharomyces cerevisiae.
Chromosome fragmentation, ATP1 disruption, and Southern blot analyses of total DNAs and prime clones of chromosome II showed that three identical ATP1s are present, directing from the telomere to the centromere on the 35-55 kb far from the left telomere sequence of chromosome II. That is, the coding and 5'-, 3'-non-coding regions of ATP1 are repeated 3 times at approximately 7 kb intervals. These three ATP1s are expressed, and one and two ATP1s-disrupted strains, respectively, showed ca.70 and 40% decreases in their ATPase activities and alpha subunit contents, compared to those of the wild type, DC-5 or W303-1A strain, but could grow on glycerol.
Takeda
M
M
Department of Applied Microbial Technology, Kumamoto Institute of Technology.
Okushiba
T
T
Satoh
T
T
Kuniyoshi
S
S
Morishita
C
C
Ichimura
Y
Y
D002874:Chromosome Mapping; D015825:Chromosomes, Fungal; D004271:DNA, Fungal / Q000032:analysis / Q000235:genetics*; D046911:Macromolecular Substances; D008928:Mitochondria / Q000648:ultrastructure; D006180:Proton-Translocating ATPases / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014170:Transformation, Genetic
D004271:DNA, Fungal; D046911:Macromolecular Substances; D006180:Proton-Translocating ATPases