Polylysine enhances cationic liposome-mediated transfection of the hepatoblastoma cell line Hep G2.
Plasmid DNA condensed by polylysine enhanced cationic-liposome-mediated transfection of Hep G2 cells. The luciferase expression plasmid pCMVL was complexed with the polycation poly-L-lysine and mixed with liposomes that contained a 1:1 molar ratio of the cationic lipid 1,2-dioleoyloxy-3-trimethyl-ammoniumpropane, with the neutral phospholipid 1,2-di-(cis-9-octadecenoyl)-sn-glycero-3-phosphoethanolamine. Polylysine enhanced cationic-liposome-mediated transfection of the hepatoblastoma cell line Hep G2 9-fold compared with pCMVL complexed alone with liposomes. The ratio of cationic to anionic charge of the polylysine-pCMVL complexes, and the quantity of cationic liposomes, are important determinants for optimal transfection of Hep G2 cells.
Mack
K D
KD
Department of Microbiology, University of California, Davis 95616, USA.
Walzem
R L
RL
Lehmann-Bruinsma
K
K
Powell
J S
JS
Zeldis
J B
JB
D002412:Cations; D004587:Electrophoresis, Agar Gel; D005229:Fatty Acids, Monounsaturated / Q000737:chemistry / Q000378:metabolism; D018197:Hepatoblastoma / Q000473:pathology*; D006801:Humans; D008081:Liposomes; D008113:Liver Neoplasms / Q000473:pathology*; D008156:Luciferases / Q000378:metabolism; D010316:Particle Size; D010714:Phosphatidylethanolamines / Q000737:chemistry / Q000378:metabolism; D010957:Plasmids / Q000378:metabolism; D011107:Polylysine / Q000378:metabolism / Q000494:pharmacology*; D000644:Quaternary Ammonium Compounds / Q000737:chemistry / Q000378:metabolism; D014162:Transfection; D014407:Tumor Cells, Cultured
D002412:Cations; D005229:Fatty Acids, Monounsaturated; D008081:Liposomes; D010714:Phosphatidylethanolamines; D000644:Quaternary Ammonium Compounds; D011107:Polylysine; C020888:1,2-dielaidoylphosphatidylethanolamine; D008156:Luciferases; C070046:1,2-dioleoyloxy-3-(trimethylammonium)propane