[An effective method of purifying recombinant human tumor necrosis factor alpha].
An efficient and productive isolation method for human recombinant tumor necrosis factor alpha from Escherichia coli cells was developed. The method includes a membrane filtration step, two steps of ion-exchange chromatography, and gel filtration on a Sephadex G-25 column. The target product was obtained with approximately 50% total yield and greater than 95% purity according to PAGE and HPLC.
Tikhonov
R V
RV
Iakimov
S A
SA
Korobko
V G
VG
Vul'fson
A N
AN
D000595:Amino Acid Sequence; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000302:isolation & purification*
D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha
Effektivnyĭ metod ochistki rekombinantnogo alpha-faktora nekroza opukholeĭ cheloveka.