title: A competitive enzyme immunoassay for the pyrrolizidine alkaloids of the senecionine type.
abstract: We developed an immunoassay with antibodies against retrorsine to detect the closely related senecionine, the main alkaloid in various Asteraceae. Sensitivity is about 23 pg (68 fmol) for senecionine. Cross reactivity of monocrotalin, retrorsine N-oxide, senkirkine (< 0.1%), and seneciphylline (3.6-34.5%) was resolved. The alkaloid content in leaves of Petasites hybridus (Asteraceae) was determined to be 3.86 ppm, calculated as senecionine, which was far less than in rhizomes (104.8 ppm). Additionally, we proved specificity by taking immunograms from different parts of Petasites hybridus. The test is a suitable tool for assessing the toxic potential of medicinal plants containing senecionine.
authors.affiliation: Institut für Botanik und Lebensmittelkunde, Veterinärmedizinische Universität Wien, Austria.
authors.forename: T
authors.initials: T
authors.lastname: Langer
authors.forename: E
authors.initials: E
authors.lastname: Möstl
authors.forename: R
authors.initials: R
authors.lastname: Chizzola
authors.forename: R
authors.initials: R
authors.lastname: Gutleb
mesh_terms: D000818:Animals; D000906:Antibodies; D000972:Antineoplastic Agents, Phytogenic / Q000032:analysis / Q000737:chemistry; D003429:Cross Reactions; D007124:Immunoenzyme Techniques; D015394:Molecular Structure; D018515:Plant Leaves; D011763:Pyrrolizidine Alkaloids / Q000032:analysis* / Q000737:chemistry; D011817:Rabbits; D012680:Sensitivity and Specificity
chemical_list: D000906:Antibodies; D000972:Antineoplastic Agents, Phytogenic; D011763:Pyrrolizidine Alkaloids; C009237:senecionine; C003300:retrorsine