Involvement of macrophages in the development of encephalomyocarditis (EMC) virus-induced diabetes in mice.
The role of macrophages in the development of diabetes following infection with encephalomyocarditis (EMC) virus was examined in 3 strains of mice (DBA/2 and BALB/c: susceptible, C57BL/6: resistant). After infection with 100 plaque forming units (PFU)/head of EMC-D (highly diabetogenic variant), the incidence of diabetes at 3 days post infection (DPI) (DBA/2: 7/8, BALB/c: 3/8, C57BL/6: 0/8) was well correlated with the severity of macrophage infiltration with beta cell damage in the pancreatic islets (DBA/2: sever, BALB/c: moderate, C57BL/6: slight). Silica-pretreatment depleted macrophage infiltration in the pancreatic islets and decreased the incidence of diabetes at 7 DPI from 100% to 40% in DBA/2 and from 80% to 0% in BALB/c mice, respectively. These results suggest that macrophages play a critical role in the process of pancreatic beta cell damage in EMC virus infection in mice.
Hirasawa
K
K
Department of Biomedical Science, Faculty of Agriculture, University of Tokyo, Japan.
Takeda
M
M
Itagaki
S
S
Doi
K
K
D000818:Animals; D018188:Cardiovirus Infections / Q000473:pathology / Q000503:physiopathology*; D003921:Diabetes Mellitus, Experimental / Q000473:pathology / Q000503:physiopathology*; D004686:Encephalomyocarditis virus; D007515:Islets of Langerhans / Q000187:drug effects / Q000473:pathology; D008264:Macrophages / Q000187:drug effects / Q000473:pathology / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D012822:Silicon Dioxide / Q000494:pharmacology
D012822:Silicon Dioxide