Copy-dependent and position-independent expression of rat aldolase A gene.
In order to understand the molecular mechanisms of the temporal and spatial differences of gene expression in higher organisms, rat aldolase A gene carrying two distinct promoters was introduced into fertilized eggs and the resulting transgenic mice were analyzed. The transgene expression is tissue-specific and is developmentally regulated. In addition, the expression is regulated in a copy-dependent manner irrespective of where the transgene is integrated, suggesting that a mechanism excluding the effect of the integration site exists within the transgene itself. To explore the conformational change of this gene in the genome, the DNase I hypersensitive sites of the gene were examined. Three sites (DHS-1,2, and 3) were identified upstream and downstream of the gene and these sites were retained in the transgene as well as in the gene observed endogenously.
Hashido
K
K
Department of Bioscience, National Cardiovascular Center Research Institute, Osaka.
Arai
Y
Y
Kajihara
S
S
Joh
K
K
Yatsuki
H
H
Hori
K
K
Miyazaki
J
J
Yamamura
K
K
Mukai
T
T
D000818:Animals; D001665:Binding Sites; D003850:Deoxyribonuclease I / Q000378:metabolism; D005634:Fructose-Bisphosphate Aldolase / Q000235:genetics*; D015971:Gene Expression Regulation, Enzymologic; D051379:Mice; D008822:Mice, Transgenic; D009690:Nucleic Acid Conformation; D051381:Rats; D012680:Sensitivity and Specificity
D003850:Deoxyribonuclease I; D005634:Fructose-Bisphosphate Aldolase