title: Toxin-coregulated pilus, but not mannose-sensitive hemagglutinin, is required for colonization by Vibrio cholerae O1 El Tor biotype and O139 strains.
abstract: The relative contributions of toxin-coregulated pilus (TCP) and cell-associated mannose-sensitive hemagglutinin (MSHA) to the colonization ability of Vibrio cholerae O1 El Tor biotype strains and O139 Bengal strains was determined by using isogenic parental and in-frame deletion mutant pairs in the infant mouse cholera model. Both the El Tor and O139 tcpA mutant strains showed a dramatic defect in colonization as indicated by their competitive indices, whereas deletion of mshA had a negligible effect on colonization in either background.
authors.affiliation: Department of Microbiology, Dartmouth Medical School, Hanover, New Hampshire 03755, USA.
authors.forename: K H
authors.initials: KH
authors.lastname: Thelin
authors.forename: R K
authors.initials: RK
authors.lastname: Taylor
mesh_terms: D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000502:physiology*; D015373:Bacterial Typing Techniques; D001483:Base Sequence; D002771:Cholera / Q000209:etiology; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D036781:Fimbriae Proteins; D010861:Fimbriae, Bacterial / Q000502:physiology; D006388:Hemagglutinins / Q000235:genetics / Q000502:physiology*; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D017384:Sequence Deletion; D013045:Species Specificity; D014734:Vibrio cholerae / Q000235:genetics / Q000472:pathogenicity* / Q000502:physiology; D014774:Virulence / Q000235:genetics / Q000502:physiology
chemical_list: D001425:Bacterial Outer Membrane Proteins; D017931:DNA Primers; D004269:DNA, Bacterial; D006388:Hemagglutinins; C085807:TcpA protein, Vibrio cholerae; D036781:Fimbriae Proteins