Sequence determinants of 3A-mediated resistance to enviroxime in rhinoviruses and enteroviruses.
Using site-directed mutagenesis of the 3A coding region of rhinovirus 14, we have expanded our analysis of resistance to enviroxime. We have observed that high and low levels of drug resistance involve two domains within 3A and that the amino acid at position 30 is critical in determining resistance.
Heinz
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BA
Infectious Disease Research, Lilly Research Laboratories, Indianapolis, Indiana 46285, USA.Heinz_Beverly_A@Lilly,com
Vance
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LM
D000595:Amino Acid Sequence; D000998:Antiviral Agents / Q000494:pharmacology*; D001562:Benzimidazoles / Q000494:pharmacology*; D001665:Binding Sites; D004352:Drug Resistance, Microbial / Q000235:genetics; D004770:Enterovirus / Q000187:drug effects* / Q000235:genetics; D029822:Enterovirus B, Human / Q000187:drug effects; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D010091:Oximes; D012229:Rhinovirus / Q000187:drug effects* / Q000235:genetics; D013449:Sulfonamides; D014764:Viral Proteins / Q000378:metabolism
D000998:Antiviral Agents; D001562:Benzimidazoles; D010091:Oximes; D013449:Sulfonamides; D014764:Viral Proteins; C021595:viroxime