[Modification of chorionic carcinoma cells by immunomodulators. Cytokines and chorionic carcinoma cells].
OBJECTIVE
The role of inflammatory cytokines on regulation of hCG biosynthesis was studied.


METHODS
JAR choriocarcinoma cells were cultured and treated with interleukin-1 (IL-1), IL-3. IL-4, stem cell factor (SCF), tumor necrosis factor (TNF), and granulocyte or granulocyte-macrophage-colony stimulating factor. hCG concentration was determined by immunoradiometric methods and cell number was enumerated by means of an electronic particle counter.


RESULTS
Il-1 and TNF increased biosynthesis of holo-hCG as well as the free alpha- and free beta-subunit and the beta-core protein. Proliferation was not affected by any cytokine tested.


CONCLUSIONS
Inflammatory cytokines are able to increase the biosynthesis of hCG.
Marth
C
C
Univ. Klinik für Frauenheilkunde, Innsbruck.
Berger
P
P
Zwierzina
H
H
Daxenbichler
G
G
D002455:Cell Division / Q000502:physiology*; D002460:Cell Line; D002822:Choriocarcinoma / Q000473:pathology*; D006063:Chorionic Gonadotropin / Q000096:biosynthesis*; D016207:Cytokines / Q000502:physiology*; D005260:Female; D006801:Humans; D011247:Pregnancy; D014407:Tumor Cells, Cultured / Q000473:pathology*; D014594:Uterine Neoplasms / Q000473:pathology*
D006063:Chorionic Gonadotropin; D016207:Cytokines
Beeinflussung von Chorionkarzinomzellen durch Immunmodulatoren. Zytokine und Chorionkarzinomzellen.