Rapid method for visual identification of specific DNA sequences based on DNA-tagged liposomes.
We describe a rapid method for visually determining specific DNA sequences at femtomole concentrations. Liposomes, encapsulating a red dye and labeled with oligonucleotide, were used in a capillary migration-sandwich hybridization assay. Capture probe was immobilized on nitrocellulose strips, and liposomes, migrating along each strip, formed a visually discernible band in the presence of target DNA. One femtomole of synthetic target sequence could be detected in < 10 min. Sufficiently stringent hybridization conditions can be used to allow the discrimination of a 10% mismatch sequence from perfectly complementary DNA. A 366-base PCR product was detected at 200 fmol.
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Analytical Chemistry Laboratories, Cornell University, Geneva, NY 14456-0462, USA.
Montagna
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Durst
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D001483:Base Sequence; D004247:DNA / Q000032:analysis* / Q000737:chemistry*; D015342:DNA Probes; D004279:DNA, Viral / Q000032:analysis / Q000737:chemistry; D007242:Herpesvirus 1, Bovine / Q000235:genetics; D006801:Humans; D008081:Liposomes; D008301:Maleimides; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010714:Phosphatidylethanolamines; D016133:Polymerase Chain Reaction; D013696:Temperature
D015342:DNA Probes; D004279:DNA, Viral; D008081:Liposomes; D008301:Maleimides; D010714:Phosphatidylethanolamines; C043592:maleimide; C007510:1,2-dipalmitoyl-3-phosphatidylethanolamine; D004247:DNA