Integrin alphavbeta3 is expressed in selected microvessels after focal cerebral ischemia.
The endothelial and smooth muscle integrin alphaVbeta3, a receptor for vitronectin and fibrinogen, participates in angiogenesis associated with wound healing and tumorigenicity. The microvascular expression of alphavbeta3 and fibrin during experimental middle cerebral artery occlusion and reperfusion in a nonhuman primate model was examined by computer-assisted video imaging microscopy. No microvascular expression of alphavbeta3 was seen in the control subjects (n = 3) or the non-ischemic basal ganglia of subjects undergoing 2-hour MCA:O (middle cerebral artery occlusion) or 3-hour occlusion with 1-hour (n = 3), 4-hour (n = 3), and 24-hour (n = 3) reperfusion. In the ischemic territory, alphavbeta3 appeared initially at 2 hours of middle cerebral artery occlusion. Up-regulation of alphavbeta3 was confined to the media of 30.0- to 50.0-micron-diameter arterioles in the ischemic core and correlated significantly with fibrin deposition in those vessels (P < 0.0005). Integrin alphavbeta3 and its ligand fibrinogen appear in a subpopulation of microvessels after focal cerebral ischemia.
Okada
Y
Y
Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California, USA.
Copeland
B R
BR
Hamann
G F
GF
Koziol
J A
JA
Cheresh
D A
DA
del Zoppo
G J
GJ
D000818:Animals; D001479:Basal Ganglia / Q000737:chemistry; D001923:Brain Chemistry; D004730:Endothelium, Vascular / Q000737:chemistry; D005337:Fibrin / Q000032:analysis; D007150:Immunohistochemistry; D002546:Ischemic Attack, Transient / Q000378:metabolism / Q000473:pathology*; D008297:Male; D008833:Microcirculation / Q000378:metabolism*; D010215:Papio; D019030:Receptors, Vitronectin / Q000032:analysis*; D015424:Reperfusion; D015854:Up-Regulation
D019030:Receptors, Vitronectin; D005337:Fibrin