Temporal expression of retinoic acid receptors in hamster fetus during organogenesis and alteration by retinoic acid treatment.
Retinoic acid receptors alpha, beta and gamma (RAR alpha, beta, gamma) mRNAs from whole 8- to 15-day-old hamster fetuses were characterized and quantitated by Northern blots and solution hybridization using riboprobes from cloned hamster RAR cDNAs, derived from 12-day fetal hamster library. Two RAR alpha transcripts of approximately 3.1 and approximately 3.5 kb, one transcript of RAR beta approximately 2.8 kb and one transcript of RAR gamma approximately 3.1 kb were observed. The relative abundance levels of these transcripts were RAR gamma > beta > alpha. RAR beta and gamma levels peaked at day 11, increasing approximately 4-fold (beta) and approximately 2.5-fold (gamma) above their initial values at day 8. RAR alpha did not change appreciably and peaked on day 14 at 1.7 x of its lowest level at day 9. Regulation patterns of the three RARs diverged between days 8 and 9 and 13 and 14 postcoitum (p.c.) and coordinately increased between days 9 and 13 and decreased between days 14 and 15 p.c. In 12-day-old conceptuses exposed to all-trans-retinoic acid, RAR alpha did not increase significantly, but RAR beta increased 12-fold at 4 hr and RAR gamma 2-fold at 1 hr after the maternal treatments.
Colon-Teicher
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Department of Veterinary Science, Utah State University, Logan 84322-5600, USA.
Dugyala
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Sharma
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D000818:Animals; D015152:Blotting, Northern; D003001:Cloning, Molecular; D006224:Cricetinae; D018076:DNA, Complementary / Q000032:analysis; D005314:Embryonic and Fetal Development / Q000187:drug effects*; D005260:Female; D005333:Fetus / Q000737:chemistry*; D007641:Keratolytic Agents / Q000633:toxicity*; D011247:Pregnancy; D012333:RNA, Messenger / Q000032:analysis / Q000187:drug effects; D018168:Receptors, Retinoic Acid / Q000032:analysis / Q000187:drug effects* / Q000235:genetics; D014158:Transcription, Genetic; D014212:Tretinoin / Q000633:toxicity*
D018076:DNA, Complementary; D007641:Keratolytic Agents; D012333:RNA, Messenger; D018168:Receptors, Retinoic Acid; D014212:Tretinoin