Use of PCR to isolate genes encoding sigma54-dependent activators from diverse bacteria.
Degenerate PCR probes were used to amplify gene fragments encoding the catalytic domain of sigma54-dependent transcription activators. The procedure should be widely applicable, as it recovered both known and novel gene fragments: 5 from Rhizobium meliloti, 13 from Myxococcus xanthus, and 3 from Bacillus subtilis. No fragments were obtained from Synechococcus sp. strain PCC 7002 or Saccharomyces cerevisiae.
Kaufman
R I
RI
Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, Pennsylvania 16802, USA.
Nixon
B T
BT
D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000235:genetics*; D001483:Base Sequence; D000458:Cyanobacteria / Q000235:genetics*; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D016941:Myxococcus xanthus / Q000235:genetics*; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods*; D051877:RNA Polymerase Sigma 54; D012441:Saccharomyces cerevisiae / Q000235:genetics; D012808:Sigma Factor / Q000235:genetics*; D016962:Sinorhizobium meliloti / Q000235:genetics*; D015534:Trans-Activators / Q000235:genetics*
D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; D012808:Sigma Factor; D015534:Trans-Activators; D012321:DNA-Directed RNA Polymerases; D051877:RNA Polymerase Sigma 54