title: Evaluation of surfactant treatment strategies after prolonged graft storage in lung transplantation.
abstract: We have previously documented alterations in endogenous surfactant after lung transplantation and improved graft function in some dogs after instillation of bovine lipid extract surfactant (bLES) into the recipient. To determine the effect of bLES delivery method and timing of treatment on physiologic response and surfactant recovery, 21 canine left lung grafts were divided into four groups: (1) Treatment of the donor for 3 h with aerosolized bLES prior to graft storage (Donor Aerosol); (2) Treatment of the recipient with instilled bLES immediately after transplantation (Recipient Instilled); (3) No bLES treatment (Control); and (4) Aerosolized bLES in donors and instilled bLES in recipients (Combined Therapy). Aerosolized bLES was labeled with [3H]-dipalmitoylphosphatidylcholine (DPPC) and instilled bLES with [14C]-DPPC. Grafts were stored for 36 h, transplanted and reperfused for 6 h. The native right and transplanted left lungs were then lavaged and protein yield, surfactant aggregates, and bLES recovery were measured. After 6 h of reperfusion, PO2/FlO2 ratio was significantly better after Combined Therapy (372 +/- 52 mm Hg) than in the Recipient Instilled (117 +/- 47 mm Hg) and Control groups (87 +/- 26 mm Hg), with intermediate values in Donor Aerosol dogs (232 +/- 64 mm Hg). The recovery of donor aerosolized bLES from transplanted lungs was increased in dogs given Combined Therapy versus Donor Aerosol treatment alone (p = 0.03). Furthermore, with Combined Therapy there was an increased percentage of instilled bLES recovered from transplanted lungs compared with the Recipient Instilled group. We conclude that surfactant treatment strategies influence physiologic response and bLES recovery after prolonged lung preservation. Treatment of lung donors with exogenous surfactant prior to graft storage was associated with less severe lung injury. Combined donor and recipient bLES therapy resulted in a superior physiologic response during reperfusion in this model.
authors.affiliation: Transplantation-Immunobiology Group, Robarts Research Institute, University Hospital, London, Ontario, Canada.
authors.forename: R J
authors.initials: RJ
authors.lastname: Novick
authors.forename: J
authors.initials: J
authors.lastname: MacDonald
authors.forename: R A
authors.initials: RA
authors.lastname: Veldhuizen
authors.forename: F
authors.initials: F
authors.lastname: Wan
authors.forename: J
authors.initials: J
authors.lastname: Duplan
authors.forename: L
authors.initials: L
authors.lastname: Denning
authors.forename: F
authors.initials: F
authors.lastname: Possmayer
authors.forename: A A
authors.initials: AA
authors.lastname: Gilpin
authors.forename: L J
authors.initials: LJ
authors.lastname: Yao
authors.forename: D
authors.initials: D
authors.lastname: Bjarneson
authors.forename: J F
authors.initials: JF
authors.lastname: Lewis
mesh_terms: D000336:Aerosols; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D002245:Carbon Dioxide / Q000097:blood; D004285:Dogs; D007322:Instillation, Drug; D016040:Lung Transplantation; D009926:Organ Preservation; D010100:Oxygen / Q000097:blood; D011663:Pulmonary Surfactants / Q000008:administration & dosage* / Q000032:analysis; D013997:Time Factors; D014019:Tissue Donors
chemical_list: D000336:Aerosols; D011663:Pulmonary Surfactants; D002245:Carbon Dioxide; D010100:Oxygen