Cell surface polysaccharide and protein antigens of an encapsulated Porphyromonas gingivalis 16-1 strain.
We have extracted the cell surface polysaccharide antigen (PS) and protein antigen from an encapsulated and virulent strain of Porphyromonas gingivalis 16-1. The PS was separated from lipopolysaccharide (LPS) by Sephacryl S-300 gel chromatography. It was distinguished from LPS by immunodiffusion with homologous rabbit antiserum. The PS reacted specifically with serogroup b antiserum against P. gingivalis 16-1 and Su63 strains, but not with antisera against serogroup a (ATCC 33277) or serogroup c (W83). The outer membrane protein was extracted with the Zwittergent 3-16 and sarcosyl detergents in a Tris-EDTA buffer. SDS-Page analysis revealed that the extracted protein antigen of P. gingivalis 16-1 contained 2 immunodominant proteins with molecular weights of 41 KDa and 27 KDa.
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Department of Microbiology, Tokyo Dental College, Chiba, Japan.
Naito
Y
Y
Okuda
K
K
D000942:Antigens, Bacterial / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification; D006801:Humans; D005779:Immunodiffusion; D016056:Immunodominant Epitopes; D008070:Lipopolysaccharides / Q000737:chemistry; D008970:Molecular Weight; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000302:isolation & purification; D016966:Porphyromonas gingivalis / Q000737:chemistry / Q000276:immunology* / Q000472:pathogenicity; D014774:Virulence
D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D016056:Immunodominant Epitopes; D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial