Human calcitonin has the same inhibitory effect on osteoclastic bone resorption by human giant cell tumor cells as salmon calcitonin.
Human calcitonin (hCT) has been reported to have a less hypocalcemizing effect on rats and to have a lower binding affinity for the receptor of mouse osteoclasts than salmon CT(sCT). In this study we comparatively examined the effect of hCT and sCT on osteoclastic bone-resorbing activity of unfractionated cells obtained from human giant cell tumor of bone and from rabbit and mouse long bones. We found that hCT had the same inhibitory effect as sCT on the bone-resorbing activity of human and rabbit osteoclastic cells, but a different one on that of mouse cells. These results indicate that the activity of drugs should be assayed using human cells if possible.
Iida
S
S
Department of Orthopedic Surgery, Saitama Medical School, Moroyama, Saitama 350-04.
Kakudo
S
S
Mori
Y
Y
Matsui
M
M
Magota
K
K
Kitajima
Y
Y
Nakamura
N
N
Mano
H
H
Hakeda
Y
Y
Azuma
H
H
Kurokawa
T
T
Kumegawa
M
M
D000818:Animals; D001859:Bone Neoplasms / Q000473:pathology; D001862:Bone Resorption / Q000188:drug therapy*; D002116:Calcitonin / Q000494:pharmacology / Q000627:therapeutic use*; D002458:Cell Fractionation; D015726:Giant Cells / Q000166:cytology / Q000473:pathology* / Q000648:ultrastructure; D006801:Humans; D051379:Mice; D008854:Microscopy, Electron; D010010:Osteoclasts / Q000166:cytology / Q000187:drug effects* / Q000648:ultrastructure; D011817:Rabbits; D013045:Species Specificity; D014407:Tumor Cells, Cultured
C028815:salmon calcitonin; D002116:Calcitonin