title: Detection of fluorescence in situ hybridization on human metaphase chromosomes by near-field scanning optical microscopy.
abstract: Fluorescence in situ hybridization signals on human metaphase chromosomes are detected by a near-field scanning optical microscope. This makes it possible to localize and identify several fluorescently labeled genomic DNA fragments on a single chromosome with a resolution superior to traditional fluorescence microscopy. Several nucleic acid probes have been used. The hybridization signals are well resolved in the near-field fluorescence images, and the exact location of the probes can be correlated to the topography as it is afforded by the shear-force feedback.
authors.affiliation: Department of Applied Physics, University of Twente, Enschede, The Netherlands.
authors.forename: M H
authors.initials: MH
authors.lastname: Moers
authors.forename: A G
authors.initials: AG
authors.lastname: Ruiter
authors.forename: A
authors.initials: A
authors.lastname: Jalocha
authors.forename: N F
authors.initials: NF
authors.lastname: van Hulst
mesh_terms: D002878:Chromosomes, Human, Pair 1 / Q000648:ultrastructure*; D015342:DNA Probes; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008297:Male; D018625:Microscopy, Atomic Force / Q000379:methods*
chemical_list: D015342:DNA Probes