title: Expression and characterization of lacrimal gland water channels in Xenopus oocytes.
abstract: Lacrimal glands transport fluid for secretion as tears. To examine whether the expression of the aquaporin family of water channels is essential for water transport in lacrimal glands, we determined the water permeability in Xenopus oocytes injected with rat lacrimal gland poly(A)+ RNA. In oocytes injected with poly(A)+ RNA, osmotic water permeability was 4-fold higher than that observed in vehicle-injected controls. The enhanced water permeability was inhibited by 65% by coinjection of poly(A)+ RNA with antisense aquaporin-5 but not antisense aquaporin-1 oligonucleotide. To detect aquaporin mRNA in rat lacrimal glands, we performed reverse transcription-polymerase chain reaction analysis. PCR products were detected using specific aquaporin-5 primers. Our results strongly suggest that rat lacrimal glands express aquaporin-5 water channels for lacrimation.
authors.affiliation: Discovery Research Division, Santen Pharmaceutical Co., Ltd., Nara, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Ishida
authors.forename: J
authors.initials: J
authors.lastname: Maruo
authors.forename: S
authors.initials: S
authors.lastname: Mita
mesh_terms: D000818:Animals; D051398:Aquaporin 1; D051402:Aquaporin 5; D020346:Aquaporins; D001483:Base Sequence; D002463:Cell Membrane Permeability; D017931:DNA Primers; D005260:Female; D007473:Ion Channels / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D007700:Kinetics; D007765:Lacrimal Apparatus / Q000378:metabolism*; D008297:Male; D008565:Membrane Proteins; D008845:Microinjections; D008969:Molecular Sequence Data; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D009865:Oocytes; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D014981:Xenopus
chemical_list: C496022:Aqp1 protein, rat; C496040:Aqp5 protein, rat; D051402:Aquaporin 5; D020346:Aquaporins; D017931:DNA Primers; D007473:Ion Channels; D008565:Membrane Proteins; D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger; D051398:Aquaporin 1