Mass spectrometry, high performance liquid chromatography, and brain peptides.
This paper is a personal recollection of some of the events and research that surrounded the amino acid sequence determination of the hypothalamic releasing factor, TRF (now known as TRH), by mass spectrometry (MS), and the development of reverse phase high performance liquid chromatography (RP-HPLC) MS and tandem MS (MS/MS) methods for the qualitative and quantitative analysis of native opioid neuropeptides in human pituitary tissue extracts.
Desiderio
D M
DM
Department of Neurology, Charles B. Stout Neuroscience Mass Spectrometry Laboratory, University of Tennessee, Memphis 38163, USA.
D000595:Amino Acid Sequence; D001923:Brain Chemistry / Q000502:physiology; D002851:Chromatography, High Pressure Liquid; D004723:Endorphins / Q000032:analysis / Q000737:chemistry; D004745:Enkephalins / Q000032:analysis / Q000737:chemistry; D006801:Humans; D013058:Mass Spectrometry; D009479:Neuropeptides / Q000032:analysis / Q000737:chemistry*; D018847:Opioid Peptides / Q000032:analysis / Q000737:chemistry; D010902:Pituitary Gland / Q000737:chemistry*; D010906:Pituitary Hormone-Releasing Hormones / Q000032:analysis / Q000737:chemistry*; D016339:Spectrometry, Mass, Fast Atom Bombardment
D004723:Endorphins; D004745:Enkephalins; D009479:Neuropeptides; D018847:Opioid Peptides; D010906:Pituitary Hormone-Releasing Hormones