Altered release of endothelin-1,2 and thromboxane B2 from trophoblastic cells in pre-eclampsia.
The aim of the study was to investigate whether pre-eclampsia is associated with an altered release of vasoactive substances from trophoblastic cells in vitro. Trophoblastic cells from 15 uncomplicated control pregnancies and 18 pre-eclamptic pregnancies at preterm (weeks 31-36; n = 12) and term (weeks 37-40; n = 21) were cultured for 5 days. The concentrations of angiotensin II (AII), endothelin-1,2 (ET-1,2), thromboxane B2 (TXB2), 6-keto-prostaglandin F1 alpha (6-keto-PGF1 alpha) and leukotriene B4 (LTB4) were measured daily in culture media for 5 days by radioimmunoassay. In pre-eclampsia, concentrations of ET-1,2 were decreased (P < 0.01) at both preterm and term, TXB2 concentrations were increased (P < 0.05) only at preterm and the TXB2-6-keto-PGF1 alpha ratio was increased at both preterm and term (P < 0.01) as compared with the controls. Concentrations of AII, 6-keto-PGF1 alpha and LTB4 were similar to the controls. The data suggest that pre-eclampsia is associated with a decreased release of ET-1 and an increased release of TXB2 from trophoblastic cells in vitro.
Cervar
M
M
Department of Obstetrics and Gynecology, Karl-Franzens-University of Graz, Austria.
Kainer
F
F
Jones
C J
CJ
Desoye
G
G
D015121:6-Ketoprostaglandin F1 alpha / Q000378:metabolism; D000328:Adult; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured / Q000378:metabolism; D016232:Endothelins / Q000378:metabolism*; D011464:Epoprostenol / Q000378:metabolism; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007975:Leukotriene B4 / Q000378:metabolism; D011225:Pre-Eclampsia / Q000378:metabolism* / Q000473:pathology; D011247:Pregnancy; D011263:Pregnancy Trimester, Third / Q000378:metabolism; D011863:Radioimmunoassay; D013928:Thromboxane A2 / Q000378:metabolism; D013929:Thromboxane B2 / Q000378:metabolism*; D014327:Trophoblasts / Q000166:cytology / Q000378:metabolism*; D014665:Vasodilator Agents / Q000378:metabolism
D016232:Endothelins; D014665:Vasodilator Agents; D007975:Leukotriene B4; D013929:Thromboxane B2; D013928:Thromboxane A2; D015121:6-Ketoprostaglandin F1 alpha; D011464:Epoprostenol