The T cell leukemia oncoprotein SCL/tal-1 is essential for development of all hematopoietic lineages.
The T cell leukemia oncoprotein SCL/tal-1, a basic-helix-loop-helix transcription factor, is required for production of embryonic red blood cells in the mouse yolk sac. To define roles in other lineages, we studied the hematopoietic potential of homozygous mutant SCL/tal-1 -/- embryonic stem cells upon in vitro differentiation and in vivo in chimeric mice. Here we show that in the absence of SCL/tal-1, hematopoiesis, Including the generation of red cells, myeloid cells, megakaryocytes, mast cells, and both T and B lymphoid cells, is undetectable. These findings suggest that SCL/tal-1 functions very early in hematopoietic development, either in specification of ventral mesoderm to a blood cell fate, or in formation or maintenance of immature progenitors.
Porcher
C
C
Division of Hematology and Oncology, Childrens Hospital, Boston, Massachusetts, USA.
Swat
W
W
Rockwell
K
K
Fujiwara
Y
Y
Alt
F W
FW
Orkin
S H
SH
D000818:Animals; D001402:B-Lymphocytes / Q000502:physiology; D001483:Base Sequence; D051792:Basic Helix-Loop-Helix Proteins; D019070:Cell Lineage / Q000502:physiology; D002678:Chimera; D018076:DNA, Complementary / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D004912:Erythrocytes / Q000502:physiology; D015870:Gene Expression / Q000502:physiology; D006410:Hematopoiesis / Q000235:genetics*; D015458:Leukemia, T-Cell; D008264:Macrophages / Q000502:physiology; D008407:Mast Cells / Q000502:physiology; D008533:Megakaryocytes / Q000502:physiology; D051379:Mice; D008969:Molecular Sequence Data; D009154:Mutation / Q000502:physiology; D010641:Phenotype; D011518:Proto-Oncogene Proteins / Q000502:physiology; D012190:Retroviridae; D000073942:T-Cell Acute Lymphocytic Leukemia Protein 1; D013601:T-Lymphocytes / Q000502:physiology; D014157:Transcription Factors; D014162:Transfection
D051792:Basic Helix-Loop-Helix Proteins; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D011518:Proto-Oncogene Proteins; D000073942:T-Cell Acute Lymphocytic Leukemia Protein 1; D014157:Transcription Factors; C489366:Tal1 protein, mouse