title: Human cytomegalovirus is not implicated in benign prostatic hyperplasia: a study using immunohistochemistry and the polymerase chain reaction.
abstract: OBJECTIVE
  To evaluate whether human cytomegalovirus (CMV) may play a role in the pathogenesis of benign prostatic hyperplasia.
  
  
  METHODS
  We used immunohistochemistry and the polymerase chain reaction (PCR) to evaluate the presence of CMV in normal prostatic tissue (n = 12) and BPH tissue (n = 21).
  
  
  RESULTS
  Immunostaining for CMV in prostatic tissue was negative; however, staining was evident in mononuclear cells seen in the tissues of both groups. With nested PCR, CMV genomic material was demonstrated in 1 tissue specimen from each group (p > 0.1).
  
  
  CONCLUSIONS
  These data suggest that CMV is unlikely to be a significant factor in the pathogenesis of BPH.
authors.affiliation: Matsunaga-Conte Prostate Cancer Research Center, Scott Department of Urology, Baylor College of Medicine, Houston, Texas 77030, USA.
authors.forename: A M
authors.initials: AM
authors.lastname: Stapleton
authors.forename: R H
authors.initials: RH
authors.lastname: Williams
authors.forename: T L
authors.initials: TL
authors.lastname: Timme
authors.forename: G
authors.initials: G
authors.lastname: Yang
authors.forename: L D
authors.initials: LD
authors.lastname: Truong
authors.forename: T C
authors.initials: TC
authors.lastname: Thompson
mesh_terms: D001483:Base Sequence; D003587:Cytomegalovirus / Q000235:genetics / Q000302:isolation & purification*; D004279:DNA, Viral / Q000032:analysis; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011470:Prostatic Hyperplasia / Q000821:virology*
chemical_list: D004279:DNA, Viral