JAK2 is constitutively associated with c-Kit and is phosphorylated in response to stem cell factor.
Stem cell factor (SCF) interacts with the receptor tyrosine kinase c-Kit and has potent effects on hematopoiesis. We have examined the role of JAK2 in the SCF signal transduction pathway. JAK2 and c-Kit were constitutively associated, and treatment with SCF resulted in rapid and transient tyrosine phosphorylation of JAK2. Incubation of cells with JAK2 antisense oligonucleotides resulted in significant decreases in SCF-induced proliferation. These data suggest that JAK2 plays a role in SCF-induced proliferation.
Linnekin
D
D
Laboratory of Leukocyte Biology, National Cancer Institute - Frederick Cancer Research and Development Center, Md 21702 (USA).
Weiler
S R
SR
Mou
S
S
DeBerry
C S
CS
Keller
J R
JR
Ruscetti
F W
FW
Ferris
D K
DK
Longo
D L
DL
D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D016373:DNA, Antisense; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D053614:Janus Kinase 2; D051379:Mice; D008969:Molecular Sequence Data; D010750:Phosphoproteins / Q000032:analysis; D010766:Phosphorylation / Q000187:drug effects; D019000:Phosphotyrosine / Q000378:metabolism; D011505:Protein-Tyrosine Kinases / Q000378:metabolism*; D011518:Proto-Oncogene Proteins; D019009:Proto-Oncogene Proteins c-kit / Q000378:metabolism*; D015398:Signal Transduction; D019089:Stem Cell Factor / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D016373:DNA, Antisense; D010750:Phosphoproteins; D011518:Proto-Oncogene Proteins; D019089:Stem Cell Factor; D019000:Phosphotyrosine; D011505:Protein-Tyrosine Kinases; D019009:Proto-Oncogene Proteins c-kit; C507924:JAK2 protein, human; C507926:Jak2 protein, mouse; D053614:Janus Kinase 2