Autoantibody against Na+/I- symporter in the sera of patients with autoimmune thyroid disease.
Using recombinant rat Na+/I- symporter (NaIS) protein, we have immunochemically searched for the autoantibody in the sera from patients with autoimmune thyroid disease. We found that 22 out of 26 sera (84%) from patients with Graves' disease and 3 out of 20 sera (15%) from patients with Hashimoto's thyroiditis recognized it. By Western blot, these IgGs reacted with 80 kDa protein in FRTL-5 cell membrane, which co-migrated with the band stained by rabbit antibody toward NaIS. These results indicate that autoantibody against NaIS, newly identified antibody, frequently exists in patients with autoimmune thyroid disease, especially in Graves' disease.
Endo
T
T
Third Department of Internal Medicine, Yamanashi Medical University, Japan.
Kogai
T
T
Nakazato
M
M
Saito
T
T
Kaneshige
M
M
Onaya
T
T
D000818:Animals; D000906:Antibodies; D001323:Autoantibodies / Q000097:blood*; D001483:Base Sequence; D015153:Blotting, Western; D002352:Carrier Proteins / Q000032:analysis / Q000276:immunology*; D017931:DNA Primers; D006111:Graves Disease / Q000097:blood / Q000276:immunology*; D006801:Humans; D008565:Membrane Proteins / Q000032:analysis / Q000276:immunology*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011817:Rabbits; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000032:analysis / Q000276:immunology; D012016:Reference Values; D012044:Regression Analysis; D027981:Symporters; D013967:Thyroiditis, Autoimmune / Q000097:blood / Q000276:immunology*; D000099209:Sodium-Iodide Symporters
D000906:Antibodies; D001323:Autoantibodies; D002352:Carrier Proteins; D017931:DNA Primers; D008565:Membrane Proteins; D011993:Recombinant Fusion Proteins; D027981:Symporters; D000099209:Sodium-Iodide Symporters