Giardia duodenalis: exposure to metronidazole inhibits competitive interactions between isolates of the parasite in vitro.
The competitive interactions of genetically distinct isolates of Giardia duodenalis with different growth rates were studied in vitro. Electrophoretic analysis of mixed cultures showed that competition between 2 cloned isolates occurs under normal in vitro culture conditions, with faster-growing isolates outcompeting those with slower growth rates. The addition of sublethal concentrations of metronidazole to clonal mixtures in vitro prevented the competitive exclusion, which was seen in normal culture. This apparently occurred because the drug reduced the growth rate of the faster-growing but not the slower-growing clone.
Thompson
R C
RC
World Health Organization Collaborating Centre for the Molecular Epidemiology of Parasitic Infections, School of Veterinary Studies, Murdoch University, Western Australia, Australia.
Lymbery
A J
AJ
Pearce
D A
DA
Finn
K C
KC
Reynoldson
J A
JA
Meloni
B P
BP
D000818:Animals; D000981:Antiprotozoal Agents / Q000494:pharmacology*; D004592:Electrophoresis, Starch Gel; D014644:Genetic Variation; D005872:Giardia / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics / Q000254:growth & development; D006593:Hexokinase / Q000032:analysis / Q000235:genetics; D006801:Humans; D008795:Metronidazole / Q000494:pharmacology*; D010733:Phosphoglucomutase / Q000032:analysis / Q000235:genetics
D000981:Antiprotozoal Agents; D008795:Metronidazole; D006593:Hexokinase; D010733:Phosphoglucomutase