title: Two-dimensional crystallization of rabbit C-reactive protein on lipid monolayers.
abstract: Two-dimensional (2D) crystals of rabbit C-reactive protein (CRP) have been obtained by protein binding on lipid monolayers at the air/water interface. Two different types of crystalline arrays of CRP were obtained, by specific binding and non-specific adsorption to the lipids. Electron crystallographic analysis of the negatively stained specimens showed that the unit cell parameters of the CRP 2D crystals formed by specific binding were a=81 angstroms, b=78 angstroms, gamma=118.35 degrees, and those formed by nonspecific adsorption were a=74 angstroms, b=67 angstroms, gamma=95.5 degrees, both with the layer group p1. Projection maps were obtained at a resolution of 26 angstroms and 22 angstroms respectively. They showed that only the monomers of the CRP were packed in the 2D arrays and the orientations of the monomers on the lipid monolayers were different in the two types of crystals. By comparing the two projection maps, a preliminary shape of the CRP monomer has been derived. A model of the pentameric structure of the oligomeric CRP has been proposed.
authors.affiliation: State Key Laboratory of Biomembrane and Membrane Biotechnology, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing, China. absssf@tsinghua.edu.ch
authors.forename: S F
authors.initials: SF
authors.lastname: Sui
authors.forename: Z
authors.initials: Z
authors.lastname: Liu
authors.forename: W
authors.initials: W
authors.lastname: Li
authors.forename: C
authors.initials: C
authors.lastname: Xiao
authors.forename: S
authors.initials: S
authors.lastname: Wang
authors.forename: Q
authors.initials: Q
authors.lastname: Gao
authors.forename: Q
authors.initials: Q
authors.lastname: Zhou
mesh_terms: D000327:Adsorption; D000818:Animals; D002097:C-Reactive Protein / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure; D018360:Crystallography, X-Ray; D050356:Lipid Metabolism; D011485:Protein Binding; D011487:Protein Conformation; D011817:Rabbits
chemical_list: D002097:C-Reactive Protein