[The analysis of GPI in chimeric mice of ES cells (MESPU 13)].
To estimate the differential potentiality of ES (Embryonic Stem) cell line MESPU 13, the heart, liver, spleen, lung, kidney, pancreas, gonad, muscle and blood of 19 chimeric mice were analyzed for GPI (Glucose Phosphate Isomeras) marker, in these studies, type A band from the ES cells, appeared parallel to the coat color chimerism of the mice. When coat color chimerism is below 40%, type A band was not seen except in the kidney of a few mice. Type A band was detected in nearly all the organs and tissues, when coat color chimerism was over 85%, indicated the ES cell has a fairly high potential to differentiate into cells of endoderm, mesoderm and ectoderm. In addition, only type A band was observed in the muscles of 6 mice which coat color chimerism is over 85%, the results indicated that these muscles were differentiated only from ES cells.
Wu
B
B
College of Life Sciences, Peking University, Beijing.
Xian
M
M
Shang
K
K
Wu
H
H
D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D002678:Chimera; D004622:Embryo, Mammalian / Q000166:cytology*; D005956:Glucose-6-Phosphate Isomerase / Q000032:analysis*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D013234:Stem Cells / Q000201:enzymology*
D005956:Glucose-6-Phosphate Isomerase