title: The humoral immune response of turbot to recently isolated pathogenic Enterococcus strains. Cross-reactivity with other Gram-positive bacteria.
abstract: An Enterococcus sp. causing severe mortalities among farmed turbot (Scophthalmus maximus) has recently been detected in northwest Spain. We found that specific turbot serum antibodies raised against isolate RA-99.1 of the new pathogen by intraperitoneal immunization, did not cross react in enzyme-linked immunosorbent assay (ELISA) with other enterococcal or non-enterococcal Gram-positive bacteria. In immunoblotting, antibodies raised against strain RA-99.1 recognized the same components in the homologous total soluble antigen preparation (TSA) as in TSA of two other isolates of the pathogen obtained from different farms. Anti-RA-99.1 serum also recognized some components of the TSA of several other Gram-positive bacteria. Immunogold labelling indicated that the antigens which provoke the humoral immune response to this pathogen are located mainly on the bacterial surface.
authors.affiliation: Departamento de Microbiología y Parasitología, Universidad de Santiago de Compostela, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Leiro
authors.forename: A E
authors.initials: AE
authors.lastname: Toranzo
authors.forename: J
authors.initials: J
authors.lastname: Estevez
authors.forename: J
authors.initials: J
authors.lastname: Lamas
authors.forename: J L
authors.initials: JL
authors.lastname: Barja
authors.forename: F M
authors.initials: FM
authors.lastname: Ubeira
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D003429:Cross Reactions; D016983:Enterococcus / Q000276:immunology*; D005393:Fish Diseases / Q000276:immunology* / Q000382:microbiology; D005412:Flatfishes / Q000276:immunology* / Q000382:microbiology; D016908:Gram-Positive Bacterial Infections / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D008854:Microscopy, Electron / Q000662:veterinary
chemical_list: D000907:Antibodies, Bacterial