Rapid assay of cytotoxicity using Tetramitus flagellates.
A simple test for measuring cytotoxic agents has been developed using the flagellate phenotype of Tetramitus rostratus. The test measures dose-dependent inhibition of cell division by individual agents such as 4-nitroquinoline-N-oxide and other mutagens. Dose-response data are given also for mixtures including coal tar pitch condensate, centrifuged particles obtained from tap water, and water concentrates prepared with XAD-2 resin. Because Tetramitus flagellates have a gullet and are particle-feeders, the assay allows for cytotoxic measurements of whole particles without prior extraction or solvent substitution procedures. The cytotoxic activities observed may reflect genotoxic activity, since all chemicals that produced a positive response are genotoxic in other test systems.
Jaffe
R L
RL
Environmental Toxicology Laboratory, Long Island City, New York 11101, USA.
D015112:4-Nitroquinoline-1-oxide / Q000633:toxicity; D000611:Aminobiphenyl Compounds / Q000633:toxicity; D000818:Animals; D002104:Cadmium / Q000633:toxicity; D004305:Dose-Response Relationship, Drug; D056890:Eukaryota / Q000187:drug effects* / Q000254:growth & development; D009153:Mutagens / Q000633:toxicity; D010655:Phenylenediamines / Q000633:toxicity; D011137:Polystyrenes / Q000633:toxicity; D018675:Toxicity Tests; D014867:Water
D000611:Aminobiphenyl Compounds; D009153:Mutagens; D010655:Phenylenediamines; D011137:Polystyrenes; D002104:Cadmium; D014867:Water; C006757:4-biphenylamine; D015112:4-Nitroquinoline-1-oxide; C003771:Amberlite XAD-2 resin; C029728:4-phenylenediamine