Effects of growth hormone injection to embryos on growth and myosin heavy chain isoforms in growing turkeys (Meleagris gallopavo).
Effects of embryonic imprinting with growth hormone (GH) on growth and myosin heavy chain (MyHC) isoforms in pectoralis muscle were determined by injecting turkey embryos with ovine growth hormone (oGH) at a dose of 10 micrograms three times a day. Injections were made on days 20 and 26 (Treatment 1), days 14 and 20 (Treatment 2) or days 14 and 26 (Treatment 3) of incubation. In Treatment 1 poults, plasma GH concentrations were elevated at 3 days posthatch and in Treatment 3 poults, plasma GH concentrations were elevated at 15 days posthatch, as compared to control poults. At 4 weeks of age, in males, body weights, shank length and weights of pectoralis, gastrocnemius and sartorius muscles were increased in Treatment 3, and in females, body weights, shank length and weights of gastrocnemius muscle of female turkeys were increased in Treatment 1. The growth rate of female turkeys from 4 weeks through 16 weeks was increased by Treatment 1. Treatment 1 resulted in a delay in the transition from the embryonic MyHC isoform to the neonatal MyHC isoform and to the adult MyHC isoform. Treatment 3 induced an earlier appearance of the adult MyHC isoform. No effects on body and muscle growth and MyHC isoforms were observed by Treatment 2.
Maruyama
K
K
Gene Evaluation and Mapping Laboratory, United States Department of Agriculture, Beltsville, MD 20705, USA.
Kanemaki
N
N
Proudman
J A
JA
D000818:Animals; D001835:Body Weight / Q000187:drug effects; D004625:Embryo, Nonmammalian / Q000502:physiology*; D004797:Enzyme-Linked Immunosorbent Assay; D005121:Extremities / Q000033:anatomy & histology / Q000254:growth & development; D005260:Female; D006128:Growth / Q000187:drug effects*; D013006:Growth Hormone / Q000097:blood / Q000494:pharmacology*; D007536:Isomerism; D008297:Male; D024510:Muscle Development; D018482:Muscle, Skeletal / Q000187:drug effects / Q000254:growth & development; D018995:Myosin Heavy Chains / Q000737:chemistry / Q000378:metabolism*; D012756:Sheep; D014422:Turkeys / Q000196:embryology / Q000254:growth & development*
D013006:Growth Hormone; D018995:Myosin Heavy Chains