[Comparison of different methods for determination of proliferative activity of breast carcinomas].
OBJECTIVE
Different methods for evaluation of nuclear atypia and proliferative activity were compared in fifty cases of breast cancer.


METHODS
Beneath histopathologic grading, ploidy of the stem cell line and S-phasefraction were measured by flow cytometry. Ki67-index was determined immunohistochemically and by image cytometry on smears grade of malignancy and 2cDI were measured.


RESULTS
Only Ki67-index was correlated with histopathological grading. The various proliferative indices (Ki67-index, S-phase-fraction, 2cDI) were not correlated. Between image- and flow-cytometric DNA-analysis significant differences were found.


CONCLUSIONS
With the immunohistochemical determination of Ki67-index in conjunction with image-cytometry a morphologic control of the material investigated is possible. So in comparison with flow-cytometry a more differentiated analysis can be performed.
Mink
D
D
Universitätsfrauenklinik und Poliklinik mit Hebammenlehranstalt Homburg/Saar.
Villena-Heinsen
C
C
Tossounidis
I
I
Schmidt
W
W
D014408:Biomarkers, Tumor / Q000032:analysis*; D001940:Breast / Q000473:pathology; D001943:Breast Neoplasms / Q000473:pathology*; D002455:Cell Division / Q000502:physiology*; D002467:Cell Nucleus / Q000473:pathology; D004273:DNA, Neoplasm / Q000032:analysis; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007091:Image Processing, Computer-Assisted; D019394:Ki-67 Antigen; D009363:Neoplasm Proteins / Q000032:analysis; D009687:Nuclear Proteins / Q000032:analysis; D011003:Ploidies; D011379:Prognosis; D016196:S Phase / Q000502:physiology
D014408:Biomarkers, Tumor; D004273:DNA, Neoplasm; D019394:Ki-67 Antigen; D009363:Neoplasm Proteins; D009687:Nuclear Proteins
Vergleich unterschiedlicher Methoden zur Bestimmung der proliferativen Aktivität von Mammakarzinomen.