Evidence for a link between translocation and processing during protein import into soybean mitochondria.
The effect of metal chelators on protein import was investigated using isolated soybean mitochondria and soybean precursor proteins. Adding 1,10-phenanthroline, a metal chelator that can cross both mitochondrial membranes abolished import of both the alternative oxidase, and the F(A)d subunit of the ATP synthase, a matrix located protein. Other metal chelators such as EDTA, 1,7-phenanthroline and 4,7-phenanthroline, which cannot cross the mitochondrial membranes, had no effect on import. When processing, a known metal-dependent step inside mitochondria, was inhibited using a mutagenesis approach (changing a -2 arginine to a -2 glycine in the pre-piece of the precursor), so was import. Thus it would appear that in soybean, at least, translocation of proteins across the mitochondrial membrane, as well as processing, relies on a metal dependent step. Taken together, the data suggest the two processes may be directly connected in these mitochondria.
Whelan
J
J
Department of Biochemistry, University of Western Australia, Nedlands, Perth, Australia.
Tanudji
M R
MR
Smith
M K
MK
Day
D A
DA
D000595:Amino Acid Sequence; D001483:Base Sequence; D001692:Biological Transport / Q000187:drug effects; D002614:Chelating Agents / Q000494:pharmacology; D008928:Mitochondria / Q000378:metabolism*; D024101:Mitochondrial Proteins; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009154:Mutation; D010088:Oxidoreductases / Q000378:metabolism; D010618:Phenanthrolines / Q000494:pharmacology; D010940:Plant Proteins / Q000378:metabolism*; D011498:Protein Precursors / Q000378:metabolism; D011499:Protein Processing, Post-Translational / Q000502:physiology*; D006180:Proton-Translocating ATPases / Q000737:chemistry / Q000378:metabolism; D013025:Glycine max / Q000378:metabolism; D000099188:Alternative Oxidase
D002614:Chelating Agents; D024101:Mitochondrial Proteins; D010088:Oxidoreductases; D010618:Phenanthrolines; D010940:Plant Proteins; D011498:Protein Precursors; D006180:Proton-Translocating ATPases; D000099188:Alternative Oxidase; C025205:1,10-phenanthroline