Establishment of a new extrahepatic bile duct carcinoma cell line, TFK-1.
A new human extrahepatic bile duct carcinoma cell line (TFK-1) was established from a surgically resected tumor specimen, which was histologically diagnosed as partly papillary adenocarcinoma and partly differentiated tubular adenocarcinoma. The tumor cells cultured in RPMI-1640 medium supplemented with 10% FBS grew as monolayers showing epithelial-like morphology with a population doubling time of 37 hr during exponential growth at passage 40. Chromosome number was distributed in the range of 72 to 76, with a modal number of 73. Tumor markers (CEA, CA19-9, ST-439, DUPAN-2) were negative in culture supernatant and plasma of SCID mice grafted with TFK-1 cells. Though no point mutation at 12 codon of K-ras was detected, expression of c-erb B-2 product and MUC1 antigen was positive. TFK-1 is the third cell line established from extrahepatic bile duct carcinomas in the world literature, and should provide useful information on various aspects of this type of neoplasm.
Saijyo
S
S
First Department of Surgery, Tohoku University School of Medicine, Sendai, Japan.
Kudo
T
T
Suzuki
M
M
Katayose
Y
Y
Shinoda
M
M
Muto
T
T
Fukuhara
K
K
Suzuki
T
T
Matsuno
S
S
D000230:Adenocarcinoma / Q000378:metabolism / Q000473:pathology* / Q000648:ultrastructure; D000818:Animals; D001483:Base Sequence; D001650:Bile Duct Neoplasms / Q000378:metabolism / Q000473:pathology* / Q000648:ultrastructure; D017734:Bile Ducts, Extrahepatic / Q000378:metabolism / Q000473:pathology* / Q000648:ultrastructure; D014408:Biomarkers, Tumor / Q000378:metabolism; D002455:Cell Division; D004252:DNA Mutational Analysis; D005260:Female; D005434:Flow Cytometry; D011905:Genes, ras; D006801:Humans; D007150:Immunohistochemistry; D007621:Karyotyping; D008297:Male; D051379:Mice; D016513:Mice, SCID; D008875:Middle Aged; D008969:Molecular Sequence Data; D018396:Mucin-1 / Q000096:biosynthesis; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured
D014408:Biomarkers, Tumor; D018396:Mucin-1