Cloning and expression of the mouse pseudoautosomal steroid sulphatase gene (Sts).
Steroid sulphatase (STS) is an important enzyme in steroid metabolism. The human STS gene has been cloned and mapped to Xp22.3, proximal to the pseudoautosomal region (PAR). Using quantitative differences in STS activity among various mouse strains, a segregation pattern consistent with autosomal linkage was first reported, but more recent studies have linked Sts to the mouse PAR. Failed attempts to clone the mouse Sts gene using human reagants (STS cDNA and anti-STS antibodies) suggest a substantial divergence between these genes. However, partial amino-terminal sequence from purified rat liver Sts is very similar to its human counterpart, and several domains are conserved among all the sulphatases. We followed a degenerate-primer reverse transcriptase-PCR (RT-PCR) approach to amplify a conserved fragment of the rat Sts cDNA that was then used to clone the mouse Sts cDNA. This 2.3-kb cDNA revealed 75% similarity with rat Sts cDNA, while it was only 63% similar to human STS cDNA. Transfection of STS(-) A9 cells with the mouse Sts cDNA restored STS enzymatic activity. Sts was also mapped physically to the distal end of the mouse sex chromosomes, and our backcross studies placed Sts distal to the 'obligatory' cross-over in male meiosis.
Salido
E C
EC
Department of Pediatrics, UCSF School of Medicine, San Francisco, California 94143, USA.
Li
X M
XM
Yen
P H
PH
Martin
N
N
Mohandas
T K
TK
Shapiro
L J
LJ
D000595:Amino Acid Sequence; D000818:Animals; D001192:Arylsulfatases / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D006224:Cricetinae; D017931:DNA Primers; D005260:Female; D015870:Gene Expression; D008040:Genetic Linkage; D006801:Humans; D008297:Male; D051379:Mice / Q000235:genetics*; D008809:Mice, Inbred C3H; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D051381:Rats; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D043266:Steryl-Sulfatase; D014960:X Chromosome; D014998:Y Chromosome
D017931:DNA Primers; D011994:Recombinant Proteins; D001192:Arylsulfatases; D043266:Steryl-Sulfatase