title: [Determination of free d-biotin--comparison of instrumental and non-instrumental methods of analysis].
abstract: Simple methods for determining free d-biotin were developed based on the latex agglutination reaction inhibition and competitive ELISA. Biotin-binding protein Str used as a biospecific marker was immobilized on the surface of colored polyacrolein latex particles or a nitrocellulose membrane. The sensitivity of both methods of the free d-biotin assay, which take 1.5 h, is 1.0-3.0 ng/ml.
authors.forename: I S
authors.initials: IS
authors.lastname: Pavlova
authors.forename: I A
authors.initials: IA
authors.lastname: Liubavina
authors.forename: Iu V
authors.initials: IuV
authors.lastname: Lukin
authors.forename: M I
authors.initials: MI
authors.lastname: Tsibul'skaia
authors.forename: P N
authors.initials: PN
authors.lastname: Korolev
mesh_terms: D000372:Agglutination Tests; D001426:Bacterial Proteins / Q000378:metabolism; D015415:Biomarkers; D001710:Biotin / Q000032:analysis* / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D012680:Sensitivity and Specificity; D019809:Streptavidin
chemical_list: D001426:Bacterial Proteins; D015415:Biomarkers; D001710:Biotin; D019809:Streptavidin
vernacular_title: Opredelenie svobodnogo d-biotina--sravnenie instumental'nogo i neinstrumental'nogo metodov analiza.