title: Tetraphenylphosphonium chloride induced MR-visible lipid accumulation in a malignant human breast cell line.
abstract: The effect of the cationic lipophilic phosphonium salt tetraphenylphosphonium chloride (TPP) on a human malignant breast cell line, DU4475, was monitored with proton nuclear magnetic resonance (1H MRS). TPP caused a dose- and time- dependent increase in resonances arising from MR-visible lipid as measured by the CH2/CH3 ratio in the 1-dimensional 1H MR spectrum. Two-dimensional MRS identified increases in the glycerophosphocholine/lysine cross-peak ratio and corresponding decreases in the phosphocholine/lysine ratio in a dose- dependent fashion in TPP-treated cells. Lipid metabolic changes are discussed in the light of other MR experiments, and the data indicate that accumulation of MR-visible lipids may arise from the rearrangement of phospholipids accompanying mitochondrial destruction or from the catabolism of phospholipids associated with early events in the cytotoxic process.
authors.affiliation: Department of Cancer Medicne, University of Sydney, NSW, Australia.
authors.forename: E J
authors.initials: EJ
authors.lastname: Delikatny
authors.forename: S K
authors.initials: SK
authors.lastname: Roman
authors.forename: R
authors.initials: R
authors.lastname: Hancock
authors.forename: T M
authors.initials: TM
authors.lastname: Jeitner
authors.forename: C M
authors.initials: CM
authors.lastname: Lander
authors.forename: D C
authors.initials: DC
authors.lastname: Rideout
authors.forename: C E
authors.initials: CE
authors.lastname: Mountford
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D001943:Breast Neoplasms / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D050356:Lipid Metabolism; D009682:Magnetic Resonance Spectroscopy; D009861:Onium Compounds / Q000494:pharmacology*; D009943:Organophosphorus Compounds / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D009861:Onium Compounds; D009943:Organophosphorus Compounds; C013289:tetraphenylphosphonium