Sodium dodecyl sulfate (SDS)-based whole-mount in situ hybridization of Xenopus laevis embryos.
We describe a streamlined whole-mount in situ hybridization protocol that utilizes high concentrations of the detergent sodium dodecyl sulfate (SDS). Our results suggest that SDS is an effective blocking agent in Xenopus laevis embryos which permeabilizes membranes without disrupting morphology. Consequently, riboprobes appeared to disperse uniformly within the embryo and several arduous and/or laborious steps of conventional procedures could be eliminated without compromising the technique.
Shain
D H
DH
Department of Biochemistry and Molecular Biology Colorado State University, Fort Collins 8052, USA. dshain@mendel.berkeley.edu
Zuber
M X
MX
D000818:Animals; D004625:Embryo, Nonmammalian / Q000166:cytology* / Q000378:metabolism; D017403:In Situ Hybridization / Q000379:methods*; D007202:Indicators and Reagents; D015347:RNA Probes; D012967:Sodium Dodecyl Sulfate; D014982:Xenopus laevis
D007202:Indicators and Reagents; D015347:RNA Probes; D012967:Sodium Dodecyl Sulfate