title: Effect of diacylglycerols on osteoclastic bone resorption.
abstract: We studied the effect of various synthetic diacylglycerols (DAGs) on bone resorption by rat and chick osteoclasts. 1-stearoyl-2-arachidonoyl-sn-glycerol (DAG IV), at a concentration of 100 microM, caused a significant reduction in resorption pit number in both species at 6 and 24 hours without any toxic effect. Over a 6-hour incubation period, a significant inhibition was seen at 10 and 100 microM in both species. 1,2-dioctanoyl-sn-glycerol (DAG I) and 1,2-dihexanoyl-sn-glycerol (DAG III) caused a marked inhibition of resorption by rat osteoclasts at 6 hours, but there was recovery of bone-resorptive ability over a 24-hour incubation period. DAGs with the -rac conformation failed to have any effect on bone resorption. In time-lapse video studies, osteoclast motility was not influenced by any of the DAGs at any of the concentrations used. Our results indicate that DAGs with the -sn conformation inhibit bone resorption, and DAGs with the -rac conformation do not. The finding that DAGs, the physiological activators of protein kinase C (PKC), inhibit bone resorption provides further evidence for an important role of the PKC pathway in the regulation of osteoclast activity.
authors.affiliation: Regional Bone Center, Helen Hayes Hospital, New York State Department of Health, Route 9W, West Haverstraw, New York 10993, USA.
authors.forename: B S
authors.initials: BS
authors.lastname: Moonga
authors.forename: L S
authors.initials: LS
authors.lastname: Stein
authors.forename: J M
authors.initials: JM
authors.lastname: Kilb
authors.forename: D W
authors.initials: DW
authors.lastname: Dempster
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001862:Bone Resorption / Q000188:drug therapy* / Q000201:enzymology; D002465:Cell Movement / Q000187:drug effects; D002470:Cell Survival; D002642:Chick Embryo; D002645:Chickens; D004075:Diglycerides / Q000494:pharmacology / Q000627:therapeutic use*; D004789:Enzyme Activation / Q000187:drug effects; D010010:Osteoclasts / Q000166:cytology / Q000187:drug effects*; D011493:Protein Kinase C / Q000378:metabolism*; D051381:Rats; D013237:Stereoisomerism
chemical_list: D004075:Diglycerides; D011493:Protein Kinase C