title: Experimental study on choline acetyltransferase activity measurement for brachial plexus injury.
abstract: We studied choline acetyltransferase (CAT) activity in a rat model of brachial plexus injury. In experiment 1, we found that the CAT activity was remarkably high in the anterior roots and low in the posterior roots. In experiment 2, nerve root segments were extracted and examined for CAT activity in the root avulsion group (group A) and the plexus severance group (group B). CAT activity decreased day by day in group A, reaching about 1/20 of the normal 5 days postoperatively. By contrast, in group B activity decreased only slightly, and even at 90 days postoperatively was about 6,000 cpm (one-half normal level). As a result, CAT activity enabled us to distinguish postganglionic from preganglionic injury of the cervical roots. Furthermore, it was a useful adjunct for minimizing nerve loss in intercostal nerve transfer, distinguishing motor and sensory branches of the intercostal nerve, and evaluating the motor nerve activity.
authors.affiliation: Department of Orthopaedic Surgery, Nara Medical University, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Yajima
authors.forename: K
authors.initials: K
authors.lastname: Kawanishi
authors.forename: H
authors.initials: H
authors.lastname: Ohgushi
authors.forename: S
authors.initials: S
authors.lastname: Tamai
mesh_terms: D000818:Animals; D001917:Brachial Plexus / Q000293:injuries*; D002795:Choline O-Acetyltransferase / Q000378:metabolism*; D004796:Clinical Enzyme Tests; D051381:Rats; D011916:Rats, Inbred F344; D013997:Time Factors; D014947:Wounds and Injuries / Q000175:diagnosis
chemical_list: D002795:Choline O-Acetyltransferase