title: Increased urinary trypsin-inhibitory activity in mercuric chloride induced nephrotoxicity in Wistar rats.
abstract: The relationship between trypsin-inhibitory activity (TIA) and the nephrotoxic effects of mercuric chloride (HgCl2)--as illustrated by proteinuria and by a drop in the glomerular filtration rate (GFR) measured by creatinine clearance test (CCT)--was investigated in Wistar rats. HgCl2, 150 or 250 micrograms/100 g BW per day was injected intraperitoneally three times a week for 2 weeks. Both groups showed a significant degree of proteinuria and urinary TIA. Group B (250 micrograms HgCl2/100 g BW) displayed a greater drop in GFR than group A (150 micrograms HgCl2/100 g BW). The urinary TIA was significantly correlated with proteinuria (group A: r = 0.87, group B: r = 0.84), but it was also significantly inversely correlated with the CCT (A: r = -0.96; B: r = -0.88).
  
  
  CONCLUSIONS
  these results suggest that increased urinary TIA may be involved in and indicative of the pathogenesis of mercuric chloride induced nephrotoxicity.
authors.affiliation: Nephrology Institute, Wolfson Hospital, Holon, Israel.
authors.forename: S
authors.initials: S
authors.lastname: Smetana
authors.forename: S
authors.initials: S
authors.lastname: Khalef
authors.forename: L
authors.initials: L
authors.lastname: Zaidel
authors.forename: Y
authors.initials: Y
authors.lastname: Bar-Khayim
authors.forename: Y
authors.initials: Y
authors.lastname: Birk
mesh_terms: D000818:Animals; D003404:Creatinine / Q000032:analysis; D005260:Female; D007668:Kidney / Q000473:pathology; D007674:Kidney Diseases / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D008627:Mercuric Chloride / Q000506:poisoning*; D008630:Mercury Poisoning / Q000209:etiology / Q000378:metabolism* / Q000473:pathology; D011507:Proteinuria / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D014361:Trypsin Inhibitors / Q000652:urine*
chemical_list: D014361:Trypsin Inhibitors; D008627:Mercuric Chloride; D003404:Creatinine