Herd evaluation by enzyme-linked immunosorbent assay for the determination of Fasciola hepatica infection in sheep and cattle from the Altiplano of Bolivia.
A study was designed to determine by ELISA the seroprevalence of fasciolosis both in sheep (29 herds totaling 184 sheep), in samples collected in 1988, and in cattle (41 herds totaling 299 animals, samples collected in 1988; 34 herds totaling 147 animals, samples collected in 1989) in the same area of Corapata in which a seroprevalence survey had been done in humans. The results show high seropositivity in sheep (89%) and lower seropositivity in cattle (58% in 1988, and 57% in 1989). The seroprevalence in cattle in 1988 was essentially identical to that detected in 1989. Faecal examinations were also done in the 1988 sheep and 1989 cattle. Results of the study showed that of the 184 sheep examined, 22 were positive for F. hepatica eggs, while 163 were positive by serology. All of the 22 sheep which were positive parasitologically were also positive serologically for a sensitivity of 100%. On the other hand, of 147 cattle tested, 38 were positive parasitologically while 84 were positive serologically. Of the 38 positives for F. hepatica eggs, 31 were positive by serology (sensitivity 82%).
Hillyer
G V
GV
Department of Pathology and Laboratory Medicine, University of Puerto Rico School of Medicine, San Juan.
Soler de Galanes
M
M
Buchón
P
P
Bjorland
J
J
D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D001838:Bolivia / Q000453:epidemiology; D002417:Cattle; D002418:Cattle Diseases; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary; D005210:Fasciola hepatica; D005211:Fascioliasis / Q000175:diagnosis / Q000453:epidemiology / Q000662:veterinary*; D005243:Feces / Q000469:parasitology; D006801:Humans; D015995:Prevalence; D015203:Reproducibility of Results; D012756:Sheep; D012757:Sheep Diseases; D013045:Species Specificity
D000909:Antibodies, Helminth