[Fingerprinting of potato species and cultivar using RAPD-PCR].
Polymerase chain reaction with random primers (RAPD-PCR) is used to fingerprint DNA polymorphism of six species and eight cultivars of potato. Three primers were developed experimentally for detection by DNA fingerprinting of cultivars and species. High significance and reproducibility of amplified DNA spectra for each species and cultivar was demonstrated. In these spectra, RAPD-PCR fragments, were revealed which may serve as cultivar-and species-specific markers for identification and differentiation of Solanum cultivars and species.
Oganisian
A S
AS
Kochieva
E Z
EZ
Ryskov
A P
AP
D001483:Base Sequence; D016172:DNA Fingerprinting / Q000379:methods*; D017931:DNA Primers; D018744:DNA, Plant / Q000235:genetics*; D005819:Genetic Markers; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D019105:Random Amplified Polymorphic DNA Technique; D015203:Reproducibility of Results; D011198:Solanum tuberosum / Q000235:genetics*; D013045:Species Specificity
D017931:DNA Primers; D018744:DNA, Plant; D005819:Genetic Markers
Markirovanie vidov i sortov kartofelia s pomoshch'iu metoda RAPD-PCR.