Binding of manganese and iron tetraphenylporphine sulfonates to albumin is relevant to their contrast properties.
The interaction of Fe(III) and Mn(III) complexes of TPPS4 with bovine serum albumin (BSA) was studied by T1 relaxation measurements of water protons and high resolution 1H NMR of the porphyrin moieties. At excess of BSA, both metalloporphyrins bind to BSA as the high spin monomers. The relaxivity of bound MnTPPS4 is significantly higher as compared to the free form in solution. When metalloporphyrins are in excess, they aggregate at the BSA surface, up to two MnTPPS4, and up to 10-15 FeTPPS4 units per BSA globule. Bound aggregates are unable to enhance magnetic relaxation of water protons due to the antiferromagnetic coupling between metal ions in the aggregates. Therefore, the dose-effect dependences for metalloporphyrins in the range of metalloporphyrin/BSA ratio of 0 to 25 at the constant BSA concentration at pH 7.4 are characterized by a local maximum at about 2 for MnTPPS4, and a global maximum at about 3 for FeTPPS4, MnTPPS4 complex is more effective than FeTPPS4 in the whole concentration range. It is suggested that the difference in binding and aggregation properties of metalloporphyrins may be relevant to their relaxation efficiency in vivo, blood transport, and biodistribution.
Yushmanov
V E
VE
Instituto de Química de São Carlos, Universidade de São Paulo, São Carlos, Brazil.
Tominaga
T T
TT
Borissevitch
I E
IE
Imasato
H
H
Tabak
M
M
D000818:Animals; D001769:Blood; D002417:Cattle; D003287:Contrast Media / Q000737:chemistry / Q000493:pharmacokinetics*; D004305:Dose-Response Relationship, Drug; D005290:Ferric Compounds / Q000737:chemistry / Q000493:pharmacokinetics*; D006859:Hydrogen; D007089:Image Enhancement; D009682:Magnetic Resonance Spectroscopy; D008345:Manganese / Q000737:chemistry / Q000493:pharmacokinetics*; D008665:Metalloporphyrins / Q000737:chemistry / Q000493:pharmacokinetics*; D011166:Porphyrins / Q000737:chemistry / Q000493:pharmacokinetics*; D011485:Protein Binding; D011522:Protons; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000378:metabolism*; D012996:Solutions; D014018:Tissue Distribution; D014867:Water
D003287:Contrast Media; D005290:Ferric Compounds; D008665:Metalloporphyrins; D011166:Porphyrins; D011522:Protons; D012996:Solutions; D014867:Water; D012710:Serum Albumin, Bovine; C016136:tetraphenylporphine sulfonate; D008345:Manganese; D006859:Hydrogen; C056661:manganese(III)tetraphenylporphine sulfonate