[Effect of tripchlorolide (T4) of Tripterygium wilfordii Hook on the production of endothelin-1 by endothelial cells of human umbilical vein].
Tripchlorolide (T4), a single active ingredient of Tripterygium wilfordii Hook was shown to possess both anti-inflammatory and immunosuppressive actions in experimental animals. The paper reports the effect of T4 on the production of endothelin-1 (ET-1) by endothelial cells of human umbilical vein. The cells were incubated with different doses of T4 (2.5, 5, 10 and 20 ng/ml) for 24 hours. By radioimmunoassay the results showed that T4 at concentration of 5 ng/ml significantly inhibited the production of ET-1 by endothelial cell in a dose dependent trend. The significance of T4 action was discussed.
Yao
Q
Q
PUMC Hospital, CAMS, Beijing.
Zeng
Z
Z
Zhang
N
N
D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D002478:Cells, Cultured; D004224:Diterpenes / Q000302:isolation & purification / Q000494:pharmacology*; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D016232:Endothelins / Q000096:biosynthesis*; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D010616:Phenanthrenes; D029757:Tripterygium; D014471:Umbilical Veins / Q000166:cytology*
D000894:Anti-Inflammatory Agents, Non-Steroidal; D004224:Diterpenes; D004365:Drugs, Chinese Herbal; D016232:Endothelins; D007166:Immunosuppressive Agents; D010616:Phenanthrenes; C068051:tripchlorolide