Transforming growth factor alpha and epidermal growth factor expression in experimental murine polycystic kidney disease.
Cystic change in polycystic kidney disease (PKD) is associated with epithelial hyperplasia, altered fluid and electrolyte transport, and de-differentiation of renal tubular epithelium. The role of polypeptide growth factors as potential modulators of cystic change remains an area of controversy. In this study, the expression of epidermal growth factor (EGF) and transforming growth factor-alpha (TGF alpha) were assessed by immunohistochemistry and image analysis in glucocorticoid-induced PKD in the newborn mouse. Newborn C3H mice received either 200 mg/kg methylprednisolone acetate (MPA) or 0.9% saline as a control. EGF expression was not detected in significant quantities in either MPA-treated or control animals. TGF alpha, however, was expressed in immature control kidney in a largely basolateral distribution. Expression increased significantly in association with cystic change in MPA-treated animals and was localized to the apical cell surface, implying altered polarity of secretion. There is no evidence that EGF is a mitogen in this early developmental model of PKD. TGF alpha, however, may be an important mediator of cystic change in immature or de-differentiated renal tubular epithelium.
Ogborn
M R
MR
Department of Pediatrics and Child Health, University of Manitoba, Winnipeg, Canada.
Sareen
S
S
D000818:Animals; D000831:Animals, Newborn; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D004195:Disease Models, Animal; D004815:Epidermal Growth Factor / Q000096:biosynthesis*; D007150:Immunohistochemistry; D008775:Methylprednisolone / Q000031:analogs & derivatives / Q000494:pharmacology; D000077555:Methylprednisolone Acetate; D051379:Mice; D008809:Mice, Inbred C3H; D007690:Polycystic Kidney Diseases / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology; D016211:Transforming Growth Factor alpha / Q000096:biosynthesis*
D000893:Anti-Inflammatory Agents; D016211:Transforming Growth Factor alpha; D000077555:Methylprednisolone Acetate; D004815:Epidermal Growth Factor; D008775:Methylprednisolone