A study of aggregation of 9-(dicyano-vinyl)julolidine.
Di(cyano vinyl)julolidine (DCVJ) is a fluorescent probe which has been used to monitor the local mobility of its binding sites on proteins. It shows a concentration dependence of its emission spectrum in water. At higher DCVJ concentrations, a longer wavelength band appears. The latter increases relative to the shorter wavelength band as a function of increased DCVJ concentration. Absorption and excitation spectra indicate that the concentration dependent emission in the longer wavelength is a consequence of association in the ground state and subsequent excimer formation. DCVJ forms two types of complexes with gamma-cyclodextrin, one of which shows the longer wavelength emission band. Analysis of stoichiometry of association also suggests that longer wavelength emission band may be a consequence of association of two molecules of DCVJ in the gamma-cyclodextrin cavity. Possible uses of such excimer formation in biological systems have also been discussed.
Bhattacharyya
A
A
Department of Biophysics, Bose Institute, Calcutta.
Bhattacharya
K
K
Bhattacharyya
B
B
Roy
S
S
D005456:Fluorescent Dyes / Q000737:chemistry*; D009570:Nitriles / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D011807:Quinolizines / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
D005456:Fluorescent Dyes; D009570:Nitriles; D011506:Proteins; D011807:Quinolizines; C061441:9-(dicyanovinyl)julolidine