Chitin assay in aspergillus fumigatus.
Our study is an attempt to make the determination of chitin possible for large amount of samples with high sensitivity and reproducibility. These requirements are fulfilled if using acid hydrolysis to release glucosamine from chitin, evaporating the solution to remove hydrochloric acid, dissolving it in distilled water to obtain optimal concentration and assaying it fluorimetrically. The described method was used for determination of chitin in spores of Aspergillus fumigatus.
Kopecek
P
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Department of Biology, Palacký University, Olomouc, Czech Republic.
D001232:Aspergillus fumigatus / Q000737:chemistry* / Q000254:growth & development; D002686:Chitin / Q000032:analysis* / Q000737:chemistry; D005944:Glucosamine / Q000032:analysis; D006868:Hydrolysis; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods; D013172:Spores, Fungal / Q000737:chemistry
D002686:Chitin; D005944:Glucosamine