title: Ultrastructural heterogeneity of acquired intradermal melanocytic nevus cells.
abstract: The present ultrastructural evaluation of 12 acquired intradermal melanocytic nevi revealed that in contrast to the nested epithelioid melanocytic nevus cells of the upper dermis, the spindle nevus cells of the deep dermis showed perineurial differentiation, exhibiting a spindly configuration characterized by a melanosome-free cytoplasm that showed extremely slender bipolar contour and contained abundant intermediate filaments, a decreased number of cytoplasmic organelles, and, significantly, a fair number of plasmalemmal pinocytotic vesicles. The nevic corpuscles were found to consist of laminated slender cytoplasm showing subcellular conformation similar to that of the spindle nevus cells. By immunohistochemistry, many spindle nevus cells and nevic corpuscles were immunoreactive for nerve growth factor receptor. All the nevus cells were immunoreactive for vimentin and S-100 protein, and negative for protein gene product 9.5, epithelial membrane antigen, Leu-7, and myelin basic protein. Characteristically, protein gene product 9.5 immunohistochemistry revealed numerous immunoreactive axons intermingled with the spindle nevus cells in the deep portion. All the PGP9.5-immunoreactive axons were observed by immunoelectron microscope to be unmyelinated and always ensheathed by a thin cytoplasmic process of Schwann cells but not nevus cells. These findings indicate that differentiation plasticity exists in the various nevus cells, with the epithelioid nevus cells and the spindle nevus cells displaying more ultrastructural and immunophenotypical characteristics of melanocyte and perineurial cells, respectively, suggesting that a pluripotential cell of neural crest origin accounts for the histogenesis of this lesion.
authors.affiliation: First Department of Pathology, University of Tokushima School of Medicine, Japan.
authors.forename: P
authors.initials: P
authors.lastname: Yang
authors.forename: T
authors.initials: T
authors.lastname: Hirose
authors.forename: T
authors.initials: T
authors.lastname: Hasegawa
authors.forename: K
authors.initials: K
authors.lastname: Seki
authors.forename: H
authors.initials: H
authors.lastname: Nakanishi
authors.forename: K
authors.initials: K
authors.lastname: Hizawa
mesh_terms: D006801:Humans; D007150:Immunohistochemistry; D016130:Immunophenotyping / Q000379:methods; D016253:Microscopy, Immunoelectron; D018330:Nevus, Intradermal / Q000145:classification* / Q000276:immunology / Q000473:pathology / Q000648:ultrastructure*; D012583:Schwann Cells / Q000648:ultrastructure*; D014407:Tumor Cells, Cultured