[A primary observation on the effect of cell fusion on metastatic potential of tumor cells].
Mouse spleen cells transfected with pSV2 neo by CaPO4 precipitation were fused with highly metastatic cell clone (PLA801-D95) from human large cell lung cancer cell line. Hybrid cell clone PMS-2 was obtained after G418(400/ml)selection. After injection of 7 x 10(6) PMS-2 cells into nude mice, there was a tumor nodule developed, but the metastatic foci could not be found while 3 x 10(6) PLA801-D95 cells would metastasize to lung and lymph nodes after they were injected into nude mice. It might indicate that sometime mouse spleen cells could not suppress tumor formation but the metastatic potential could be suppressed by the fusion of mouse spleen cells with the lung cancer cells. The results of growth curve, serum independence and incorporation rates of H-thymidine all showed that the growth rates of parental cells were higher than those of PMS-2. Our data suggest that suppression of tumorigenicity and metastatic potential could be controlled by different kinds of genes, and the cloning of metastasis suppressor gene by subtractive hybridization is ongoing in our laboratory.
Wang
R
R
Institute of Basic Medical Sciences, CAMS, Beijing.
Yan
Y
Y
Wu
H
H
Sheng
Q
Q
Liu
H
H
D000818:Animals; D002289:Carcinoma, Non-Small-Cell Lung / Q000473:pathology*; D002459:Cell Fusion; D016147:Genes, Tumor Suppressor; D006801:Humans; D006822:Hybrid Cells; D008175:Lung Neoplasms / Q000473:pathology*; D051379:Mice; D008819:Mice, Nude; D009355:Neomycin; D009362:Neoplasm Metastasis; D011815:R Factors; D013154:Spleen / Q000166:cytology; D014162:Transfection; D014407:Tumor Cells, Cultured
D009355:Neomycin