Immunohistochemical localisation of the serotonin 5-HT2B receptor in mouse gut, cardiovascular system, and brain.
We recently reported the cloning of a new member of the serotonin 5-HT2 family, the 5-HT2B receptor. We now report the production and characterisation of a specific antiserum directed against the C-terminal portion of the mouse 5-HT2B receptor. After affinity purification, this polyclonal antibody recognises specifically the mouse 5-HT2B receptor. Immunohistochemical analysis of cryosections from various adult mouse tissues reveals a major 5-HT2B receptor expression in stomach, intestine and pulmonary smooth muscles as well as in myocardium. Furthermore, the antiserum recognises specific areas of the mouse brain, including cerebellar Purkinje cells and their projection areas.
Choi
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DS
Institut de Génétique et de Biologie Moléculaire et Cellulaire, Université L. Pasteur de Strasbourg, CNRS, INSERM, Illkirch, France.
Maroteaux
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L
D000818:Animals; D000906:Antibodies / Q000302:isolation & purification; D000918:Antibody Specificity; D001921:Brain / Q000166:cytology / Q000378:metabolism*; D002460:Cell Line; D002522:Chlorocebus aethiops; D002846:Chromatography, Affinity; D004064:Digestive System / Q000166:cytology / Q000378:metabolism*; D005753:Gastric Mucosa / Q000378:metabolism; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000166:cytology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009206:Myocardium / Q000166:cytology / Q000378:metabolism*; D009928:Organ Specificity; D011817:Rabbits; D044403:Receptor, Serotonin, 5-HT2B; D011985:Receptors, Serotonin / Q000032:analysis* / Q000096:biosynthesis; D011994:Recombinant Proteins / Q000096:biosynthesis; D013270:Stomach / Q000166:cytology; D014162:Transfection
D000906:Antibodies; D044403:Receptor, Serotonin, 5-HT2B; D011985:Receptors, Serotonin; D011994:Recombinant Proteins