title: [Molecular cloning of cellulase gene from the bacillus].
abstract: The CMCase gene from the Bacillus sp. strain E2 was cloned into E. coli DH5 alpha F' using plasmid pBR322 as a vector. Recombinant plasmid pBG3 was isolated from transformants producing CMCase, and the cloned CMCase gene was found to be in a 4.0kb HindIII fragment. The CMCase gene was subcloned in a functional state on a 2.4kb DNA fragment. The optimum pH and temperature for activity of CMCase encoded by pBG3 were pH 6.5 and 55 degrees C respectively. The CMCase could not hydrolyze any natural cellulose examined. Eighty-three percent of the CMCase was detected in the inner cellular fraction, 9% and 8% were in the periplasmic space and medium respectively. Result of Southern hybridization indicates that the cloned DNA fragment was derived from Bacillus sp. strain E2 chromosomal DNA.
authors.affiliation: Academia Sinica, Chengdu Institute of Biology.
authors.forename: J
authors.initials: J
authors.lastname: Guan
authors.forename: C
authors.initials: C
authors.lastname: Fan
authors.forename: Q
authors.initials: Q
authors.lastname: Wu
authors.forename: F
authors.initials: F
authors.lastname: Zhang
authors.forename: M
authors.initials: M
authors.lastname: Jiang
authors.forename: Y
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D001407:Bacillus / Q000235:genetics*; D015139:Blotting, Southern; D002480:Cellulase / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D005798:Genes, Bacterial
chemical_list: D002480:Cellulase