Simple high-performance liquid chromatographic method for the quantitation of 5-fluorouracil in human plasma.
A simple, rapid, specific and sensitive high-performance liquid chromatography method has been developed for quantitation of 5-fluorouracil (5-FU) in human plasma. The method involves deproteinization of a small sample volume of plasma (150 microliters) followed by HPLC on a cation-exchange resin column, Aminex HPX-87H (300 x 7.8 mm I.D.), preceded by a similar guard cartridge with UV detection at 265 nm. This method allows a good separation of 5-FU with a retention time of 24 min and a detection limit at 25 ng/ml. The calibration curve was linear from 25 to 2000 ng/ml. The coefficient of variation was < or = 4.4% for within-day reproducibility and < or = 9.5% for day-to-day reproducibility.
Compagnon
P
P
Service de Pharmacologie, CHU de Rouen, Hôpital de Boisguillaume, France.
Thiberville
L
L
Moore
N
N
Thuillez
C
C
Lacroix
C
C
D000964:Antimetabolites, Antineoplastic / Q000097:blood*; D002411:Cation Exchange Resins; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002852:Chromatography, Ion Exchange / Q000379:methods; D005472:Fluorouracil / Q000097:blood*; D006801:Humans; D013056:Spectrophotometry, Ultraviolet
D000964:Antimetabolites, Antineoplastic; D002411:Cation Exchange Resins; D005472:Fluorouracil