Modulation of IL-1 beta gene expression in the rat CNS during sleep deprivation.
We hypothesize that sleep homeostasis involves, at least in part, the immune system modulator interleukin-1 beta (IL-1 beta). Using the reverse transcription-polymerase chain reaction, IL-1 beta mRNA levels in the rat CNS were evaluated after a period of sleep deprivation. In addition, IL-1 beta gene expression was analyzed before the projected onset of activity and rest phase in free-running animals. No changes in IL-1 beta mRNA were observed in the circadian cycle, but 24 h of sleep deprivation resulted in a 2-fold increase in the level of IL-1 beta mRNA in the hypothalamus and in the brain stem compared with controls (p < 0.0002 and (p < 0.0001 respectively). The alteration in IL-1 beta mRNA levels following sleep deprivation supports the hypothesis that modulation of IL-1 beta gene expression is involved in the sleep homeostatic process.
Mackiewicz
M
M
Department of Psychiatry; Center for Sleep and Respiratory Neurobiology, Philadelphia, PA 19104-4283, USA.
Sollars
P J
PJ
Ogilvie
M D
MD
Pack
A I
AI
D000818:Animals; D001483:Base Sequence; D002490:Central Nervous System / Q000378:metabolism*; D004567:Electrodes, Implanted; D004569:Electroencephalography; D005786:Gene Expression Regulation / Q000502:physiology*; D007375:Interleukin-1 / Q000096:biosynthesis* / Q000134:cerebrospinal fluid / Q000235:genetics; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012313:RNA / Q000096:biosynthesis / Q000302:isolation & purification; D051381:Rats; D017207:Rats, Sprague-Dawley; D012890:Sleep / Q000502:physiology; D012892:Sleep Deprivation / Q000502:physiology*; D014851:Wakefulness / Q000502:physiology
D007375:Interleukin-1; D012313:RNA