Regionally specific induction of ICE mRNA and enzyme activity in the rat brain and adrenal gland by LPS.
Pro interleukin-1 beta converting enzyme (ICE) activity in the pituitary was found to be significantly increased 4 h after intraperitoneal injection of E. coli lipopolysaccharides, when distribution and inducibility of the enzyme was studied in the adult rat brain and the adrenal gland, using an artificial fluorescence peptide substrate. The same lipopolysaccharide treatment induced ICE mRNA levels in the pituitary, adrenal gland and hypothalamus as studied by reverse transcript-polymerase chain reaction.
Tingsborg
S
S
Department of Neurochemistry and Neurotaxicology, Stockholm University, Sweden.
Zetterström
M
M
Alheim
K
K
Hasanvan
H
H
Schultzberg
M
M
Bartfai
T
T
D000311:Adrenal Glands / Q000187:drug effects* / Q000201:enzymology; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D020170:Caspase 1; D003546:Cysteine Endopeptidases / Q000235:genetics*; D004790:Enzyme Induction; D006624:Hippocampus / Q000187:drug effects* / Q000201:enzymology; D007031:Hypothalamus / Q000187:drug effects* / Q000201:enzymology; D007375:Interleukin-1 / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008297:Male; D008969:Molecular Sequence Data; D010902:Pituitary Gland / Q000187:drug effects / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012016:Reference Values
D007375:Interleukin-1; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D003546:Cysteine Endopeptidases; D020170:Caspase 1