title: Sequence of the highly conserved gene encoding the human 54kDa subunit of signal recognition particle.
abstract: The complete sequence of the human gene encoding the 54kDa subunit of the signal recognition particle has been isolated from a cDNA library. Degenerate oligonucleotides based on the 5' and 3' coding region of the canine gene and a mammalian codon usage table, were used to amplify the sequence using PCR. The nucleotide sequence of the human gene shows that the human sequence shares a 95.8% nucleotide sequence homology and 100% amino acid sequence homology to it's canine counterpart. The sequence has been given the accession number X86373 in the EMBL database.
authors.affiliation: Department of Surgery, St. George's Hospital Medical School, London, UK.
authors.forename: S
authors.initials: S
authors.lastname: Patel
authors.forename: B
authors.initials: B
authors.lastname: Austen
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D017124:Conserved Sequence; D018076:DNA, Complementary / Q000235:genetics; D004285:Dogs; D015723:Gene Library; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment; D018271:Signal Recognition Particle / Q000737:chemistry / Q000235:genetics*
chemical_list: D018076:DNA, Complementary; D018271:Signal Recognition Particle