title: Rapid and selective high-performance liquid chromatographic method for the determination of metronidazole and its active metabolite in human plasma, saliva and gastric juice.
abstract: A rapid and selective HPLC method has been developed for the separation and quantitation of metronidazole and its hydroxylated metabolite in human plasma, saliva and gastric juice. The assay requires a simple protein precipitation step prior to analysis and is selective, sensitive and reproducible. The limits of quantitation (0.5-ml sample) were at least 0.25 microgram/ml for metronidazole and 0.20 micrograms/ml for its hydroxy metabolite. A Hypersil ODS 5 micron (150 x 4.5 mm I.D.) column was used with a mobile phase of acetonitrile-aqueous 0.05 M potassium phosphate buffer (pH 7) containing 0.1 % triethylamine (10:90) delivered at a flow-rate of 1.0 ml/min.
authors.affiliation: Department of Pharmaceutical Sciences, University of Nottingham, University Park, UK.
authors.forename: M J
authors.initials: MJ
authors.lastname: Jessa
authors.forename: D A
authors.initials: DA
authors.lastname: Barrett
authors.forename: P N
authors.initials: PN
authors.lastname: Shaw
authors.forename: R C
authors.initials: RC
authors.lastname: Spiller
mesh_terms: D000890:Anti-Infective Agents / Q000097:blood / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005750:Gastric Juice / Q000378:metabolism*; D006801:Humans; D008795:Metronidazole / Q000031:analogs & derivatives* / Q000097:blood / Q000378:metabolism*; D012015:Reference Standards; D015203:Reproducibility of Results; D012463:Saliva / Q000378:metabolism*; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet; D014011:Tinidazole / Q000097:blood / Q000378:metabolism
chemical_list: D000890:Anti-Infective Agents; D014011:Tinidazole; D008795:Metronidazole; C020910:1-(2-hydroxyethyl)-2-hydroxymethyl-5-nitroimidazole