The role of gastric mucosal sulphydryls in the ulcer-protecting effects of cisapride.
The present study was designed to examine the role of endogenous sulphydryls (SHs) in the gastro-protection induced by cisapride (CIS) (10, 25 and 50 mg kg-1 i.p.), a potent benzamide stimulating gastrointestinal motility in mucosal injury induced by 50% v/v ethanol. Results were compared with those of 5-hydroxytryptamine (5-HT) (10mg kg-1). Ethanol mucosal damage was significantly reduced by treatment with CIS and 5-HT. On the contrary, administration of n-ethylmaleimide (NEM) (10 mg kg-1) an SH alkylator, markedly worsened lesion formation and counteracted the protective effect of CIS. Rats pretreated with CIS significantly increased the total sulphydryls as reflected in the non-protein and protein fractions however, 5-HT treatment showed a fall in the non-protein level. The present results suggest that 5-HT-ergic dependent mechanisms have no relation to the gastro-protection afforded by CIS in this experimental model. It is possible that mucosal SHs could be involved.
López
A
A
Departamento de Farmacia y Tecnología Farmacéutica, Laboratorio de Farmacología, Sevilla, Spain.
Motilva
V
V
Alarcón de la Lastra
C
C
Martín
M J
MJ
La Casa
C
C
D000818:Animals; D000897:Anti-Ulcer Agents / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D020117:Cisapride; D004195:Disease Models, Animal; D000431:Ethanol / Q000633:toxicity; D005033:Ethylmaleimide / Q000008:administration & dosage / Q000378:metabolism / Q000633:toxicity; D005753:Gastric Mucosa / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D007274:Injections, Intraperitoneal; D007279:Injections, Subcutaneous; D008297:Male; D010880:Piperidines / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D051381:Rats; D017208:Rats, Wistar; D012701:Serotonin / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use; D013276:Stomach Ulcer / Q000139:chemically induced / Q000188:drug therapy*; D013438:Sulfhydryl Compounds / Q000032:analysis / Q000502:physiology*; D013439:Sulfhydryl Reagents / Q000008:administration & dosage / Q000378:metabolism / Q000633:toxicity
D000897:Anti-Ulcer Agents; D010880:Piperidines; D013438:Sulfhydryl Compounds; D013439:Sulfhydryl Reagents; D012701:Serotonin; D000431:Ethanol; D005033:Ethylmaleimide; D020117:Cisapride