The Escherichia coli ribosomal protein S16 is an endonuclease.
The histone-like protein HU isolated from Escherichia coli exhibited, after several purification steps, a Mg(2+)-dependent nuclease activity. We show here that this activity can be dissociated from HU by a denaturation-renaturation step, and is due to a small fraction of ribosomal protein S16 co-purifying with HU. S16 is an essential component of the 30S ribosomal particles. We have cloned, overproduced, and purified a histidine-tagged S16 and shown that this protein is a DNA-binding protein carrying a Mg(2+)-Mn(2+)-dependent endonuclease activity. This is an unexpected property for a ribosomal protein.
Oberto
J
J
Institut de Biologie Physico-chimique, Laboratoire de Physiologie Bactérienne, Paris, France.
Bonnefoy
E
E
Mouray
E
E
Pellegrini
O
O
Wikström
P M
PM
Rouvière-Yaniv
J
J
D001426:Bacterial Proteins / Q000302:isolation & purification; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D004268:DNA-Binding Proteins / Q000302:isolation & purification; D004720:Endonucleases / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D008274:Magnesium / Q000378:metabolism; D008345:Manganese / Q000378:metabolism; D008969:Molecular Sequence Data; D012269:Ribosomal Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*
D001426:Bacterial Proteins; D017931:DNA Primers; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; D012269:Ribosomal Proteins; C040330:histone-like protein HU, bacteria; C023509:ribosomal protein S16; D008345:Manganese; D004720:Endonucleases; D008274:Magnesium