[Detection of p53 mutation using PCR-SSCP silver staining method].
A simple and sensitive technique to detect point mutation of the p53 gene using silver staining method for single-strand conformation polymorphism analysis of polymerase chain reaction (PCR-SSCP) is reported. In this study, the aberrations of the p53 gene (exon 6-8) in clinical specimens of primary cervical carcinoma were examined. Of 27 tumor tissues 2 samples showed mutations of p53 exon 7. Absence of abnormal bands in the p53 exon 6-8 in all ten normal cervical tissues. It appears that inactivation of p53 gene by point mutation is infrequent in clinical samples of human cervical carcinomas.
Zhao
W
W
Institute of Basic Medical Sciences, CAMS, Beijing.
Si
J
J
Li
K
K
Zhang
X
X
Song
G
G
D001483:Base Sequence; D004273:DNA, Neoplasm / Q000235:genetics; D005091:Exons; D005260:Female; D016158:Genes, p53; D006801:Humans; D008969:Molecular Sequence Data; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D016622:Silver Staining; D002583:Uterine Cervical Neoplasms / Q000235:genetics*
D004273:DNA, Neoplasm