Platelet-activating factor and haematopoiesis. XI. Platelet-activating factor has no effect on the production of interleukin-6 and tumor necrosis factor-alpha by human bone marrow stromal cells.
PAF is a phospholipid mediator of inflammation with stimulates IL-6 production by murine skin fibroblasts. Although PAF is present in human bone marrow, its role in haematopoiesis is unknown. We have assessed whether PAF stimulates IL-6 and TNF-alpha production by human bone marrow stromal cells (mostly fibroblast-like cells). We report that PAF (1 nM to 10 microM) has no effect on the synthesis of IL-6 and TNF-alpha by human bone marrow stromal cells. This difference may be due to the widely accepted concept "tissue-specific fibroblasts". The role of PAF in the regulation of human haematopoiesis remains to be elucidated.
Denizot
Y
Y
Laboratoire d Hématologie Expérimentale, Faculté de Médecine, Limoges France.
Rougier
F
F
Cornu
E
E
Praloran
V
V
D001853:Bone Marrow / Q000187:drug effects*; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D006410:Hematopoiesis / Q000187:drug effects*; D006801:Humans; D015850:Interleukin-6 / Q000096:biosynthesis*; D010972:Platelet Activating Factor / Q000494:pharmacology*; D017154:Stromal Cells / Q000187:drug effects / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*
D015850:Interleukin-6; D010972:Platelet Activating Factor; D014409:Tumor Necrosis Factor-alpha