Characterization of tumor cell lines derived from murine gammaherpesvirus-68-infected mice.
Cell lines were derived from mice with murine gammaherpesvirus-68 (MHV-68)-associated lymphoproliferative disease. Four were of an ambiguous phenotype and were MHV-68 negative. One, S11, was a B lymphocyte that contained MHV-68 genomes in both linear and episomal forms and released virus. The line was clonable and grew into tumors in nude mice. This is the first naturally occurring MHV-68-positive B-cell line to be generated, and it will be an invaluable tool for the study of MHV-68 latency.
Usherwood
E J
EJ
Department of Veterinary Pathology, University of Edinburgh, Summerhall, United Kingdom. Edward.Usherwood@ed.ac.uk
Stewart
J P
JP
Nash
A A
AA
D000212:Acyclovir / Q000494:pharmacology; D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology; D046508:Culture Techniques / Q000379:methods; D018142:Gammaherpesvirinae / Q000187:drug effects / Q000502:physiology; D006566:Herpesviridae Infections; D008223:Lymphoma / Q000276:immunology / Q000473:pathology* / Q000821:virology; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D010641:Phenotype; D014407:Tumor Cells, Cultured; D014412:Tumor Virus Infections
D000998:Antiviral Agents; D000212:Acyclovir