Cigarette smoke-modified low density lipoprotein impairs endothelium-dependent relaxation in isolated rabbit arteries.
1. We investigated the effects of cigarette smoke-modified low-density lipoprotein (CS-LDL) on endothelium-dependent relaxations in various isolated rabbit arteries, and compared them with those of oxidized LDL (Ox-LDL). 2. In aorta rings, acetylcholine-induced endothelium-dependent relaxations were attenuated by preincubation with CS-LDL and Ox-LDL. Endothelium-independent relaxations induced by sodium nitroprusside were not modified. Similar changes were observed in rings of coronary and basilar arteries. 3. These findings indicate that CS-LDL, as well as Ox-LDL, may decrease the release or activity of endothelium-derived relaxing factor in vascular endothelial cells.
Kagota
S
S
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Mukogawa Women's University, Nishinomiya, Japan.
Yamaguchi
Y
Y
Shinozuka
K
K
Kwon
Y M
YM
Kunitomo
M
M
D000109:Acetylcholine / Q000378:metabolism; D000818:Animals; D004587:Electrophoresis, Agar Gel; D004730:Endothelium, Vascular / Q000502:physiology*; D066298:In Vitro Techniques; D008077:Lipoproteins, LDL / Q000097:blood / Q000737:chemistry / Q000494:pharmacology*; D009126:Muscle Relaxation / Q000187:drug effects; D009131:Muscle, Smooth, Vascular / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D009569:Nitric Oxide / Q000378:metabolism; D009599:Nitroprusside / Q000494:pharmacology; D010084:Oxidation-Reduction; D011817:Rabbits; D012907:Smoking / Q000097:blood*; D017392:Thiobarbituric Acid Reactive Substances / Q000737:chemistry; D014665:Vasodilator Agents / Q000494:pharmacology
D008077:Lipoproteins, LDL; D017392:Thiobarbituric Acid Reactive Substances; D014665:Vasodilator Agents; D009599:Nitroprusside; D009569:Nitric Oxide; D000109:Acetylcholine