Acute and chronic effects of estrogenic compounds on glutamate-stimulated phosphatidylinositol metabolism in primary neuronal cultures.
Glutamate (Glu)-stimulated phosphatidylinositol (PI) metabolism in primary neuronal cultures was found to be modulated by acute and chronic treatment with two estrogenic compounds. 17 beta-Estradiol 3-benzoate (0.1 and 1 microM), when applied with Glu, significantly reduced Glu (40 microM)-stimulated PI metabolism by 20-36%, an effect not seen with 17 alpha-estradiol. The weak estrogen phenol red (20 microM), had no effect when added immediately before Glu stimulations. Two-week pretreatment with 17 beta-estradiol 3-benzoate (1 microM) resulted in a significant decrease in Glu-stimulated PI metabolism (10-100 microM). Chronic treatment with 20 microM phenol red, at a concentration commonly found in culture medium, resulted in parallel but not statistically significant effects to those observed with chronic estradiol treatment. Estrogenic compounds may modulate the excitatory responses of neurons by both genomic and non-genomic means.
Yourick
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DL
Department of Medical Neurosciences, Walter Reed Army Institute of Research, Washington, DC 20307-5100, USA.
DeCoster
M A
MA
D000818:Animals; D002478:Cells, Cultured; D004958:Estradiol / Q000031:analogs & derivatives* / Q000494:pharmacology*; D005069:Evaluation Studies as Topic; D018698:Glutamic Acid / Q000494:pharmacology*; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D010637:Phenolsulfonphthalein; D010716:Phosphatidylinositols / Q000378:metabolism*; D051381:Rats; D013268:Stimulation, Chemical; D013997:Time Factors
D010716:Phosphatidylinositols; C074283:estradiol 3-benzoate; D018698:Glutamic Acid; D004958:Estradiol; D010637:Phenolsulfonphthalein