title: Phenotypic reversion of smooth muscle cells in hybrid vascular prostheses.
abstract: Our purpose was to evaluate whether or not and when phenotypic modulation of smooth muscle cells (SMCs) in hybrid vascular prostheses preincorporated with SMCs occurs upon implantation. Two types of hybrid vascular grafts incorporated with vascular cells derived from canine jugular veins were prepared: grafts containing a collagen gel layer covered with an endothelial monolayer at the luminal surface (Model I graft) and those containing an endothelial monolayer and SMC multilayer (Model II graft). They were bilaterally implanted into carotid arteries of the same dogs from which the cells had been harvested for 2 wk (n = 3) and 12 wk (n = 3). The time-dependent changes in populations of three SMC phenotypes (synthetic, intermediate, and contractile) in the neoarterial layers were quantified by morphometric evaluation using a transmission electron microscope in hybrid vascular grafts. Before implantation, all the SMCs were of the synthetic phenotype. In Model II grafts at 2 wk, synthetic and intermediate SMCs were dominant especially in the luminal layer. On the other hand, neoarterial layers at 12 wk were dominated by contractile SMCs, which were evenly distributed throughout the entire neoarterial tissues. A markedly delayed phenotypic reversion was noted for the Model I grafts at 12 wk. In the hybrid grafts, during about 3 mo of implantation, neoarterial SMCs transformed from the synthetic to the contractile phenotypes, which was promoted by SMC incorporation.
authors.affiliation: Department of Bioengineering, National Cardiovascular Center Research Institute, Osaka, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Kanda
authors.forename: H
authors.initials: H
authors.lastname: Miwa
authors.forename: T
authors.initials: T
authors.lastname: Matsuda
mesh_terms: D000818:Animals; D001158:Arteries / Q000502:physiology / Q000648:ultrastructure; D001807:Blood Vessel Prosthesis; D017690:Cell Transplantation; D002478:Cells, Cultured / Q000166:cytology / Q000502:physiology / Q000648:ultrastructure; D004285:Dogs; D004730:Endothelium, Vascular / Q000166:cytology / Q000502:physiology; D007601:Jugular Veins / Q000166:cytology; D008854:Microscopy, Electron; D009131:Muscle, Smooth, Vascular / Q000166:cytology* / Q000502:physiology; D010641:Phenotype; D012038:Regeneration / Q000502:physiology