Evaluation of a one-step test for rapid, in practice detection of rotavirus in farm animals.
An immunochromatographic test for the detection of group A rotavirus was evaluated against a reference group A rotavirus ELISA, by using a panel of 161 bovine, porcine and equine faecal samples submitted for routine examination. The sensitivity of the test was 89 per cent and the specificity 99 per cent compared with the ELISA. Its reproducibility was 100 per cent. The simplicity and rapidity of the test procedure make it suitable for use in practice.
de Verdier Klingenberg
K
K
Faculty of Veterinary Medicine, Department of Cattle and Sheep Diseases, Uppsala, Sweden.
Esfandiari
J
J
D000818:Animals; D002417:Cattle / Q000821:virology*; D002845:Chromatography; D004797:Enzyme-Linked Immunosorbent Assay; D005069:Evaluation Studies as Topic; D005243:Feces / Q000821:virology; D006736:Horses / Q000821:virology*; D007118:Immunoassay; D012015:Reference Standards; D015203:Reproducibility of Results; D012401:Rotavirus / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D013552:Swine / Q000821:virology*