title: Activation of the plasma coagulation system induced by some biomaterials.
abstract: The ability of some biomaterials to activate plasma coagulation system was examined in vitro. After contact of platelet-rich plasma with biomaterials, some markers of the thrombin formation, i.e., fragment 1 + 2 and fibrinopeptide A, and some inhibitors of the blood coagulation mechanism were tested. Fragment 1 + 2 and fibrinopeptide A were found to be increased by all of the materials, though to a different extent. In particular, fragment 1 + 2 and fibrinopeptide A were significantly increased upon contact with polybutylene terephthalate and with collagen coated polyethylene terephthalate, respectively. Also antithrombin III was shown to decrease following exposure to biomaterials, but statistical significance was found only for polyethylene terephthalate and polyvinylacetate. As a results of this wide range of variability in the parameters, it is advisable to explore the plasma coagulation system with a multiparametric approach in which thrombin formation and coagulation inhibitors are thoroughly investigated.
authors.affiliation: Laboratory for Biocompatibility Research on Implant Materials, Istituti Ortopedici Rizzoli, Bologna, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Cenni
authors.forename: G
authors.initials: G
authors.lastname: Ciapetti
authors.forename: M
authors.initials: M
authors.lastname: Cervellati
authors.forename: D
authors.initials: D
authors.lastname: Cavedagna
authors.forename: G
authors.initials: G
authors.lastname: Falsone
authors.forename: S
authors.initials: S
authors.lastname: Gamberini
authors.forename: A
authors.initials: A
authors.lastname: Pizzoferrato
mesh_terms: D000990:Antithrombin III / Q000378:metabolism; D001672:Biocompatible Materials / Q000494:pharmacology*; D001777:Blood Coagulation / Q000187:drug effects*; D001792:Blood Platelets / Q000187:drug effects / Q000502:physiology*; D003094:Collagen; D005344:Fibrinopeptide A / Q000032:analysis; D006801:Humans; D010446:Peptide Fragments / Q000378:metabolism; D010447:Peptide Hydrolases / Q000378:metabolism; D011091:Polyesters / Q000494:pharmacology; D011093:Polyethylene Terephthalates / Q000494:pharmacology; D011486:Protein C / Q000378:metabolism; D011516:Prothrombin / Q000378:metabolism; D013917:Thrombin / Q000096:biosynthesis
chemical_list: D001672:Biocompatible Materials; D010446:Peptide Fragments; D011091:Polyesters; D011093:Polyethylene Terephthalates; D011486:Protein C; C046193:antithrombin III-protease complex; C045879:prothrombin fragment 1.2; D005344:Fibrinopeptide A; C041733:poly(1,4-butylene terephthalate); D000990:Antithrombin III; D011516:Prothrombin; D003094:Collagen; D010447:Peptide Hydrolases; D013917:Thrombin