[Effect of lipid peroxide on lymphocytes and its role in postburn immunosuppression].
OBJECTIVE
Mechanisms of postburn immunosuppression are complicated and remain unclear. In the present experiment the effect of linoleic acid hydroperoxide (LOOH) on lymphocytes was evaluated in vitro, and then the changes in postburn lipid peroxide (LPO) levels and lymphocyte functions were measured, in order to investigate the mechanism of immunosuppression following burn.


METHODS
Proliferation and IL-2 production and LPO of lymphocytes were assayed after the incubation with LOOH for in vitro study. On day 6 after induction of 11%-12% TBSA full-thickness burn, the animals were sacrificed, and proliferation and IL-2 production of splenic lymphocytes, LPO levels of the plasma, livers and spleens were measured.


RESULTS
LOOH inhibited proliferation and IL-2 production and induced lipid peroxidation of lymphocytes in vitro, and Vit E could attenuate the effects of LOOH. LPO levels increased and proliferation and IL-2 production decreased after burn, but those changes in Vit E or SOD group showed no statistical significance compared with normal control group.


CONCLUSIONS
Enhancement of lipid peroxidation and increased LPO after burn may be one of the mechanisms of postburn immunosuppression.
Zhang
Q
Q
Department of Surgery, First Teaching Hospital, Beijing Medical University.
Li
T
T
Huang
Y
Y
D000818:Animals; D002056:Burns / Q000276:immunology* / Q000378:metabolism; D002455:Cell Division; D005260:Female; D007108:Immune Tolerance; D007376:Interleukin-2 / Q000096:biosynthesis; D008041:Linoleic Acids / Q000494:pharmacology; D008054:Lipid Peroxides / Q000378:metabolism* / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D013601:T-Lymphocytes / Q000166:cytology / Q000378:metabolism
D007376:Interleukin-2; D008041:Linoleic Acids; D008054:Lipid Peroxides; C008285:linoleic acid hydroperoxide