title: Accelerating the pace of luciferase reporter gene assays.
abstract: Luciferase reporter gene assays have gained more importance because of their easy readout, high sensitivity and lack of environmental waste disposal problems. However, several obstacles remain that have prohibited a wider use and the implementation of this type of assay in high-throughput screening programs: (i) Measurements need to be carried out within an active enzyme reaction, and the assessment of such reactions are time-dependent; (ii) the signal produced has a "flash" type characteristic and therefore requires specialized equipment for measurement; and (iii) side-reactions can occur that interact with the signal readout of the assay in a non-reproducible way. These hurdles make an otherwise convenient assay principle troublesome for larger-scale screening use. We have attempted to overcome these problems by different means, leading to the development of LucLite, a stable signal homogeneous reagent system. This system allows use in a higher throughput screening capacity and enables the use of standard scintillation/luminescence instruments.
authors.affiliation: Packard Instrument B. V., Groningen, The Netherlands.
authors.forename: C H
authors.initials: CH
authors.lastname: Roelant
authors.forename: D A
authors.initials: DA
authors.lastname: Burns
authors.forename: W
authors.initials: W
authors.lastname: Scheirer
mesh_terms: D001681:Biological Assay; D002460:Cell Line; D017930:Genes, Reporter / Q000235:genetics*; D005820:Genetic Testing / Q000379:methods*; D006801:Humans; D007202:Indicators and Reagents; D008156:Luciferases / Q000235:genetics*; D008163:Luminescent Measurements; D015398:Signal Transduction / Q000502:physiology; D013601:T-Lymphocytes / Q000166:cytology; D014162:Transfection
chemical_list: D007202:Indicators and Reagents; D008156:Luciferases