A model for detecting early metabolic changes in neonatal asphyxia by 1H-MRS.
In newborn rabbits, the early cerebral metabolic changes caused by hypoxic-ischemic (H-I) insult was examined by using volume localized 1H-MRS (STEAM). Partial ischemia was caused by unilateral carotid artery ligation, and hypoxia was induced by 10% oxygen inspiration for 150 minutes. Lactate immediately increased after hypoxia induction and almost disappeared 120 to 150 minutes after removal of hypoxia in both H-I and hypoxia-only experiments. Lactate production correlated well with decrease of the blood oxygen saturation. More lactate was produced on ischemic side 50 minutes post-hypoxia induction in H-I study. Ischemia alone did not cause any significant lactate production. Lactate caused by hypoxia can be dynamically monitored by localized 1H-MRS. Existence of regional ischemia can induce greater anaerobic glycolysis and may affect the pattern of brain injury under hypoxia. 1H-MRS is a sensitive tool to detect the acute metabolic change caused by H-I insult.
Nakai
T
T
Department of Radiology, Stanford University Medical Center, CA 94305-5488, USA.
Rhine
W D
WD
Enzmann
D R
DR
Stevenson
D K
DK
Spielman
D M
DM
D000818:Animals; D000831:Animals, Newborn; D001238:Asphyxia Neonatorum / Q000175:diagnosis / Q000503:physiopathology*; D001921:Brain / Q000098:blood supply; D001925:Brain Damage, Chronic / Q000175:diagnosis / Q000503:physiopathology*; D004195:Disease Models, Animal; D004292:Dominance, Cerebral / Q000502:physiology; D004734:Energy Metabolism / Q000502:physiology*; D006801:Humans; D002534:Hypoxia, Brain / Q000175:diagnosis / Q000503:physiopathology; D007091:Image Processing, Computer-Assisted; D007231:Infant, Newborn; D019344:Lactic Acid / Q000097:blood; D008279:Magnetic Resonance Imaging; D009682:Magnetic Resonance Spectroscopy; D011817:Rabbits
D019344:Lactic Acid