Requirements for green fluorescent protein detection in transgenic zebrafish embryos.
We have generated transgenic (Tg) lines of zebrafish in which the green fluorescent protein (GFP)-encoding gfp cDNA is driven by the Xenopus laevis ef1 alpha enhancer/promoter; Tg embryos from most of these lines show detectable fluorescence throughout their body. We have investigated the copy number of the Tg genes in fluorescent and non-fluorescent lines, in order to determine how this affects the production of detectable levels of GFP in the zebrafish embryo. Additionally, we have injected purified recombinant GFP into embryos to determine the intracellular GFP concentration required for detection, both when all of the cells in the embryo contain GFP and when only a few do.
Amsterdam
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A
Department of Biology, Massachusetts Institute of Technology, Cambridge 02139, USA.
Lin
S
S
Moss
L G
LG
Hopkins
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N
D000818:Animals; D030801:Animals, Genetically Modified / Q000196:embryology; D018628:Gene Dosage; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000032:analysis* / Q000235:genetics; D008845:Microinjections; D011817:Rabbits; D037401:Scyphozoa; D019076:Transgenes; D014982:Xenopus laevis; D015027:Zebrafish
D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins