[Characterization of inbred strains of rats by microsatellite analysis].
Microsatellite analysis was applied to identify the inbred rat strains LEW, SHR and WKY. Of a set of seven commercially available primer pairs three pairs were used individually and two times two in duplex polymerase chain reactions (PCR). The products were analyzed in ethidium bromide stained agarose gels. The resulting banding patterns clearly demonstrated the homogeneity within strains and the differences between strains.
Dolf
G
G
Institut für Tierzucht, Universität Bern.
Colomb
B
B
Schläpfer
J
J
Leuenberger
J
J
Märki
U
U
D000818:Animals; D004276:DNA, Satellite / Q000032:analysis / Q000235:genetics*; D004587:Electrophoresis, Agar Gel; D016133:Polymerase Chain Reaction / Q000379:methods; D051381:Rats; D011917:Rats, Inbred Lew / Q000235:genetics; D011918:Rats, Inbred SHR / Q000235:genetics; D011919:Rats, Inbred Strains / Q000235:genetics*; D011921:Rats, Inbred WKY / Q000235:genetics
D004276:DNA, Satellite
Charakterisierung von Ratten-Inzuchtlinien mit Hilfe der Mikrosatellitenanalyse.