A novel murine cathelin-like protein expressed in bone marrow.
A novel cDNA encoding a putative secreted protein was isolated from murine bone marrow. The encoded protein named MCLP (murine cathelin-like protein) was found to be highly homologous to the pig cathelin, and to four neutrophil antimicrobial polypeptides: CAP 18, indolicidin, Bac 5 and FALL-39. Secondary structure prediction studies identified a highly cationic region in the C-terminal part of prepro-MCLP with a tendency to adopt an amphipathic alpha-helical conformation, as observed in many antimicrobial peptides. However, no antibacterial activity was observed with the synthetic peptide corresponding to this region of MCLP.
Popsueva
A E
AE
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russian Federation. popsueva@imb.imb.ac.ru
Zinovjeva
M V
MV
Visser
J W
JW
Zijlmans
J M
JM
Fibbe
W E
WE
Belyavsky
A V
AV
D000595:Amino Acid Sequence; D000818:Animals; D023181:Antimicrobial Cationic Peptides; D001483:Base Sequence; D001853:Bone Marrow / Q000378:metabolism*; D054804:Cathelicidins; D003001:Cloning, Molecular; D018076:DNA, Complementary; D015870:Gene Expression; D051379:Mice; D008969:Molecular Sequence Data; D009928:Organ Specificity; D014176:Protein Biosynthesis; D011498:Protein Precursors; D017433:Protein Structure, Secondary; D011506:Proteins / Q000737:chemistry*; D017386:Sequence Homology, Amino Acid; D013552:Swine
D023181:Antimicrobial Cationic Peptides; D054804:Cathelicidins; D018076:DNA, Complementary; D011498:Protein Precursors; D011506:Proteins; C084515:cathelin