title: Ceftazidime kinetics of diffusion in inoculated agar plates.
abstract: The ceftazidime concentration in agar plates inoculated with Pseudomonas aeruginosa was determined by high-performance liquid chromatography at fixed points (3, 6, 9, 12, and 15 mm) from the disk center and at fixed times (2, 4, 6, 16, and 24 h) to study the antibiotic kinetics of diffusion. A statistical difference between the concentrations determined in the presence of microorganisms and in uninoculated plates after 16 and 24 h was evidenced and was probably ascribable to the drug hydrolysis carried out by the induced beta-lactamase.
authors.affiliation: Laboratory of Chromatography and Separative Techniques, H.S. Raffaele, Milan, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Arcelloni
authors.forename: R
authors.initials: R
authors.lastname: Paroni
authors.forename: M
authors.initials: M
authors.lastname: Basile
authors.forename: P A
authors.initials: PA
authors.lastname: Bonini
authors.forename: R
authors.initials: R
authors.lastname: Vaiani
mesh_terms: D000362:Agar; D002442:Ceftazidime / Q000032:analysis*; D004058:Diffusion; D007700:Kinetics; D008826:Microbial Sensitivity Tests; D011550:Pseudomonas aeruginosa / Q000378:metabolism*; D001618:beta-Lactamases / Q000378:metabolism
chemical_list: D000362:Agar; D002442:Ceftazidime; D001618:beta-Lactamases