Influence of hematocrit on erythrocyte aggregation kinetics for suspensions of red blood cells in autologous plasma.
The influence of hematocrit on red blood cell aggregation was investigated using a modified automated Myrenne Aggregometer System. Hematocrit values ranged from 20-70% at 10% increments, and were produced by resuspending washed red cells in autologous plasma. The Myrenne System produced seven kinetic parameters of red cell aggregation. The results indicate that hematocrit (Hct) had a strong and nonlinear effect on erythrocyte aggregability. At both low and high hematocrit, the aggregation intensity (AI) tends to decrease, reaching a plateau at 40% hematocrit. The disaggregation shear rate demonstrates an exponential relationship as In gamma TMIN = 3.29-0.0837 Hct. Time constants of the aggregation process such as T1/2, TSLOW and TFAST were rather inconsistent. The increase of hematocrit increased the rate of the fast phase aggregation and slightly slowed the secondary aggregation.
Deng
L H
LH
Bioengineering Unit, University of Strathclyde, Glasgow, UK.
Barbenel
J C
JC
Lowe
G D
GD
D001696:Biomechanical Phenomena; D004903:Erythrocyte Aggregation / Q000502:physiology*; D006400:Hematocrit; D018056:Hemorheology; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D008955:Models, Cardiovascular; D010949:Plasma / Q000502:physiology; D013314:Stress, Mechanical