An improved method for preparing the chromosomes of pines and other gymnosperms.
A simple technic is described to produce well spread gymnosperm chromosomes. Root tip meristems are digested with a pectinase:cellulase mixture to produce a cell suspension which then is squashed to yield flat, well spread chromosome complements that can be stained or used for in situ hybridization.
Murray
B G
BG
School of Biological Sciences, University of Auckland, New Zealand.
Davies
B J
BJ
D002875:Chromosomes; D005821:Genetic Techniques; D014197:Trees / Q000235:genetics*