1H and 15N nuclear magnetic resonance assignment and secondary structure of the cytotoxic ribonuclease alpha-Sarcin.
The ribosome-inactivating protein alpha-Sarcin (alpha S) is a 150-residue fungal ribonuclease that, after entering sensitive cells, selectively cleaves a single phosphodiester bond in an universally conserved sequence of the major rRNA to inactivate the ribosome and thus exert its cytotoxic action. As a first step toward establishing the structure-dynamics-function relationships in this system, we have carried out the assignment of the 1H and 15N NMR spectrum of alpha S on the basis of homonuclear (1H-1H) and heteronuclear (1H-15N) two-dimensional correlation spectra of a uniformly 15N-labeled sample, and two selectively 15N-labeled (Tyr and Phe) samples, as well as a single three-dimensional experiment. The secondary structure of alpha S, as derived from the characteristic patterns of dipolar connectivities between backbone protons, conformational chemical shifts, and the protection of backbone amide protons against exchange, consists of a long N-terminal beta-hairpin, a short alpha-helical segment, and a C-terminal beta-sheet of five short strands arranged in a + 1, + 1, + 1, + 1 topology, connected by long loops in which the 13 Pro residues are located.
Campos-Olivas
R
R
Instituto de Estructura de la Materia, Consejo Superior de Investigaciones Cientificas, Madrid, Spain.
Bruix
M
M
Santoro
J
J
Martínez del Pozo
A
A
Lacadena
J
J
Gavilanes
J G
JG
Rico
M
M
D000595:Amino Acid Sequence; D001230:Aspergillus / Q000201:enzymology*; D003603:Cytotoxins / Q000737:chemistry*; D004722:Endoribonucleases / Q000737:chemistry*; D005656:Fungal Proteins; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009587:Nitrogen Isotopes; D017433:Protein Structure, Secondary; D011522:Protons
D003603:Cytotoxins; D005656:Fungal Proteins; D009587:Nitrogen Isotopes; D011522:Protons; C014801:alpha-sarcin; D004722:Endoribonucleases