title: Monensin concentrations measured in feeder cattle using enzyme immunoassay.
abstract: Thirty heifers were fed a ration containing 30 g monensin/ton. Fecal, urinary and seral samples were collected at varying intervals prior to and after initiating administration of the monensin-containing feed, and monensin concentrations were determined using a modified indirect enzyme immunoassay. Fecal samples contained measurable (micrograms/g; ppm) concentrations of monensin in most samples. The majority of sera and urine samples contained monensin at ng/ml (ppb) concentrations, which were above background levels prior to monensin feeding. Twelve head were fed monensin at 60 g/ton and 90 g/ton for 5 d with collection of similar samples. Higher concentrations of monensin were detected with increasing ration amounts in all 3 sample types. Enzyme immunoassay for monensin in these biological samples identified presence of the feed additive.
authors.affiliation: Department of Molecular Biosciences, University of California, Davis 95616, USA.
authors.forename: M E
authors.initials: ME
authors.lastname: Mount
authors.forename: J S
authors.initials: JS
authors.lastname: Cullor
authors.forename: P H
authors.initials: PH
authors.lastname: Kass
authors.forename: W
authors.initials: W
authors.lastname: Garret
mesh_terms: D000821:Animal Feed / Q000032:analysis; D000818:Animals; D002417:Cattle / Q000378:metabolism*; D005243:Feces / Q000737:chemistry*; D005260:Female; D007124:Immunoenzyme Techniques; D008985:Monensin / Q000032:analysis* / Q000097:blood / Q000652:urine; D015203:Reproducibility of Results
chemical_list: D008985:Monensin