title: The S. cerevisiae outwardly-rectifying potassium channel (DUK1) identifies a new family of channels with duplicated pore domains.
abstract: Potassium channel subunits have six or two transmembrane segments in addition to a conserved pore-forming (P) domain; four subunits come together to form a channel. A gene was identified in S. cerevisiae (J0911) encoding a protein with eight probable membrane-spanning segments and two such P regions. This protein (Duk1p) is a potassium channel because Xenopus oocytes injected with the corresponding RNA express potassium currents activated by depolarization that are not seen in control oocytes. Similar potassium currents were recorded from wildtype S. cerevisiae spheroplasts, but not from those in which the DUK1 locus had been disrupted. Cells carrying the duk1 delta 1::HIS disruption in addition to a chimeric gene comprising DUK1 behind the GAL1 promoter showed outward currents when grown in galactose, but not when grown in glucose. Additional sequences with the duplicate pore motif were found in C. elegans, suggesting that these proteins represent a novel structural family of potassium channel proteins.
authors.affiliation: Glaxo Institute for Molecular Biology, Geneva, Switzerland.
authors.forename: J D
authors.initials: JD
authors.lastname: Reid
authors.forename: W
authors.initials: W
authors.lastname: Lukas
authors.forename: R
authors.initials: R
authors.lastname: Shafaatian
authors.forename: A
authors.initials: A
authors.lastname: Bertl
authors.forename: C
authors.initials: C
authors.lastname: Scheurmann-Kettner
authors.forename: H R
authors.initials: HR
authors.lastname: Guy
authors.forename: R A
authors.initials: RA
authors.lastname: North
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002462:Cell Membrane; D003001:Cloning, Molecular; D004553:Electric Conductivity; D005071:Evoked Potentials; D005260:Female; D005690:Galactose; D015870:Gene Expression; D005800:Genes, Fungal / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D009865:Oocytes; D015221:Potassium Channels / Q000235:genetics / Q000502:physiology*; D012331:RNA, Fungal; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins; D013104:Spheroplasts; D014982:Xenopus laevis
chemical_list: D015221:Potassium Channels; D012331:RNA, Fungal; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; C095220:TOK1 protein, S cerevisiae; D005690:Galactose