Single-step purification/solubilization of recombinant proteins: application to surfactant protein B.
Holzinger
A
A
University of Cincinnati College of Medicine, OH, USA.
Phillips
K S
KS
Weaver
T E
TE
D002021:Buffers; D015500:Chloramphenicol O-Acetyltransferase / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000302:isolation & purification*; D006639:Histidine / Q000737:chemistry; D018398:Homeodomain Proteins / Q000302:isolation & purification; D006801:Humans; D009687:Nuclear Proteins / Q000302:isolation & purification; D011510:Proteolipids / Q000302:isolation & purification*; D011663:Pulmonary Surfactants / Q000302:isolation & purification*; D011994:Recombinant Proteins / Q000302:isolation & purification*; D012995:Solubility; D000074482:Thyroid Nuclear Factor 1; D014157:Transcription Factors / Q000302:isolation & purification
D002021:Buffers; D006023:Glycoproteins; D018398:Homeodomain Proteins; D009687:Nuclear Proteins; D011510:Proteolipids; D011663:Pulmonary Surfactants; D011994:Recombinant Proteins; D000074482:Thyroid Nuclear Factor 1; D014157:Transcription Factors; D006639:Histidine; D015500:Chloramphenicol O-Acetyltransferase