Thimerosal modulates the agonist-specific cytosolic Ca2+ oscillatory patterns in single pancreatic acinar cells of mouse.
Modulation of the agonist-specific cytosolic Ca2+ oscillatory pattern by thimerosal has been investigated in single pancreatic acinar cells using patch-clamp perforated whole-cell recording to measure the calcium-dependent chloride current (I(C1)(Ca2+)). 1 microM thimerosal, which fails to evoke Ca2+ oscillation alone, clearly changed the pattern of Ca2+ oscillation from pulsatile spikes (evoked by low concentrations of activators) to sinusoidal or transient oscillations. The mimetic action of thimerosal was independent of extracellular Ca2+, was blocked by extracellular application of dithiothreitol or 10 mM caffeine, as well as by internal perfusion with heparin; but was unaffected by ruthenium red. We conclude that thimerosal modulates the agonist-specific cytosolic Ca2+ oscillatory patterns mediated by sensitizing the InsP3-induced Ca2+ release.
Wu
J
J
Department of Physiology 1, Hirosaki University School of Medicine, Japan. wujie@unm.edu
Takeo
T
T
Kamimura
N
N
Wada
J
J
Suga
S
S
Hoshina
Y
Y
Wakui
M
M
D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D002110:Caffeine / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D002712:Chlorides / Q000378:metabolism; D002766:Cholecystokinin / Q000494:pharmacology; D003600:Cytosol / Q000378:metabolism; D004229:Dithiothreitol / Q000494:pharmacology; D006493:Heparin / Q000494:pharmacology; D066298:In Vitro Techniques; D015544:Inositol 1,4,5-Trisphosphate / Q000378:metabolism; D017136:Ion Transport / Q000187:drug effects; D051379:Mice; D010179:Pancreas / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D012430:Ruthenium Red / Q000494:pharmacology; D013439:Sulfhydryl Reagents / Q000494:pharmacology*; D013849:Thimerosal / Q000494:pharmacology*
D002712:Chlorides; D013439:Sulfhydryl Reagents; D012430:Ruthenium Red; D013849:Thimerosal; D002110:Caffeine; D015544:Inositol 1,4,5-Trisphosphate; D006493:Heparin; D002766:Cholecystokinin; D000109:Acetylcholine; D002118:Calcium; D004229:Dithiothreitol