Na(+)-K(+)-ATPase activity of different regions of rat brain in response to immunization and bone marrow cytokine treatment.
The bone marrow cytokine (Bio-Immuno Modulator, BIM or BM-Fr1) has been suggested to correct immunoincompetence by modulating brain Na(+)-K(+)-ATPase. The brain region affected and mechanism of action of BM-Fr1 are unknown, however. Here we report that immunization of immunocompetent rats indirectly inhibited Na(+)-K(+)-ATPase activity (59%) in the left cerebral lobe (LC) and irrespective of BM-Fr1 treatment, stimulation of the enzyme was observed in the LC at the peak of immune response. BM-Fr1 treatment, which corrected immunoincompetence in malnourished rats, also modulated a different LC Na(+)-K(+)-ATPase profile to that seen in immunocompetent animals. Immunogen and BM-Fr1 seem to exert their influence in brain via a cytosolic inhibitor protein of Na(+)-K(+)-ATPase. Thus we suggest that (1) BM-Fr1 plays an important role in immune homeostatasis by modulating Na(+)-K(+)-ATPase activity of LC and (2) Na(+)-K(+)-ATPase is not the receptor for either immunogen or BM-Fr1.
Ghoshal
D
D
Department of Chemistry, Bose Institute, Calcutta, India.
Adhikary
G
G
Sen
P C
PC
Barua
A K
AK
D000276:Adjuvants, Immunologic / Q000494:pharmacology; D000818:Animals; D001921:Brain / Q000201:enzymology*; D016207:Cytokines / Q000494:pharmacology*; D005260:Female; D006706:Homeostasis / Q000276:immunology; D007114:Immunization; D007121:Immunocompetence; D007165:Immunosuppression Therapy; D008297:Male; D009752:Nutritional Status; D051381:Rats; D000254:Sodium-Potassium-Exchanging ATPase / Q000187:drug effects / Q000378:metabolism*
D000276:Adjuvants, Immunologic; D016207:Cytokines; D000254:Sodium-Potassium-Exchanging ATPase