Identification of an epitope for T-cells correlated with antibody response to hepatitis B surface antigen in vaccinated humans.
Two antigenic T-cell epitopes of HBsAg, designated HBs 16-31 and HBs 81-99, were identified using synthetic peptides and HBsAg-specific T-cell lines. HBs 16-31 was recognized by five HBsAg-specific T-cell lines from vaccinees with both high and low antibody titers, whereas HBs 81-99 was recognized by two T-cell lines derived from vaccinees with high antibody titers. The antibody titer against HBsAg was correlated significantly with the proliferation of vaccinee's PBLs in response to HBs 81-99 (r = 0.47) but not to HBs 16-31, suggesting that HBs 81-99 plays a critical role in anti-HBs antibody production in humans vaccinated with HBsAg.
Min
W P
WP
Department of Genetics, Medical Institute of Bioregulation, Fukuoka, Japan.
Kamikawaji
N
N
Mineta
M
M
Tana
T
T
Kashiwagi
S
S
Sasazuki
T
T
D000595:Amino Acid Sequence; D018604:Epitope Mapping / Q000379:methods; D000939:Epitopes / Q000276:immunology*; D006680:HLA Antigens / Q000235:genetics; D006510:Hepatitis B Antibodies / Q000096:biosynthesis*; D006514:Hepatitis B Surface Antigens / Q000276:immunology*; D017325:Hepatitis B Vaccines / Q000276:immunology*; D006801:Humans; D008211:Lymphocyte Cooperation / Q000276:immunology; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
D000939:Epitopes; D006680:HLA Antigens; D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens; D017325:Hepatitis B Vaccines; D010455:Peptides