[Preparation of 125I-F(ab')2-DNR-liposomes and radioimmunoassay of their specific conjugating ability].
The F(ab')2-DNR-liposomes were prepared using F(ab')2 obtained from digested monoclonal antibody against human white cells HI30 (CD45). Their affinity constant for peripheral blood mononuclear cells was determined by radioimmunoassay. Ka = 2.0 x 10(9). A good specific conjugation ability was demonstrated. In preparing the immunoliposomes, it was found that F(ab')2 coupled to the smaller liposomes with higher conjugation ratio.
Liu
J
J
Hematology Institute, CAMS and PUMC, Tianjin.
Chen
Z
Z
Liu
H
H
Fan
Q
Q
Shen
D
D
D000903:Antibiotics, Antineoplastic / Q000008:administration & dosage*; D000911:Antibodies, Monoclonal; D001667:Binding, Competitive; D003630:Daunorubicin / Q000008:administration & dosage*; D006801:Humans; D007140:Immunoglobulin Fab Fragments; D000922:Immunotoxins; D007457:Iodine Radioisotopes; D017493:Leukocyte Common Antigens / Q000276:immunology; D008081:Liposomes; D011863:Radioimmunoassay
D000903:Antibiotics, Antineoplastic; D000911:Antibodies, Monoclonal; D007140:Immunoglobulin Fab Fragments; D000922:Immunotoxins; D007457:Iodine Radioisotopes; D008081:Liposomes; D017493:Leukocyte Common Antigens; D003630:Daunorubicin