title: Elusive control.
abstract: The concept of a single rate-limiting step was proven to be too simplistic for understanding control and regulation of metabolism. Consequently, searches have identified relatively few steps with high control. Here we review a number of such searches and indicate what mechanisms may be responsible for this elusiveness of control. It turns out that this elusiveness of control has itself led to increased understanding of the roles played in metabolic control and regulation of such diverse factors as distributiveness of control, condition dependence, enzyme elasticity, homeostasis, control hierarchies, the input into a pathway, coenzyme sequestration, and redundancy and diversity of control function.
authors.affiliation: Department of Microphysiology, Free University, Amsterdam, Netherlands.
authors.forename: H V
authors.initials: HV
authors.lastname: Westerhoff
authors.forename: B N
authors.initials: BN
authors.lastname: Kholodenko
authors.forename: M
authors.initials: M
authors.lastname: Cascante
authors.forename: K
authors.initials: K
authors.lastname: Van Dam
mesh_terms: D000818:Animals; D004798:Enzymes / Q000378:metabolism; D006706:Homeostasis; D007700:Kinetics; D008433:Mathematics; D008660:Metabolism; D008954:Models, Biological
chemical_list: D004798:Enzymes