Thioesterification of platelet proteins with saturated and polyunsaturated fatty acids.
We have demonstrated that more than 20 platelet proteins can be acylated with fatty acids via thioester linkages. These include the glycoprotein IX beta chain of glycoprotein Ib, components of the von Willebrand factor receptor on the platelet surface, P-selectin, and alpha subunits of Gz, Gq, and Gi. Our studies have shown that platelet proteins can be posttranslationally acylated in thioester linkages not only with palmitate but with myristate and also with the eicosanoid precursor fatty acids arachidonate and eicosapentaenoate. Thioesterification of platelet proteins with fatty acids other than palmitate may have significant functional consequences for reversible binding of proteins to membranes.
Laposata
M
M
Massachusetts General Hospital/Harvard Medical School, Boston 02114, USA.
Muszbek
L
L
D016718:Arachidonic Acid / Q000097:blood; D001792:Blood Platelets / Q000378:metabolism*; D001798:Blood Proteins / Q000378:metabolism*; D015118:Eicosapentaenoic Acid / Q000097:blood; D004951:Esterification; D005227:Fatty Acids / Q000097:blood*; D005231:Fatty Acids, Unsaturated / Q000097:blood*; D006801:Humans; D019814:Myristic Acid; D009227:Myristic Acids / Q000097:blood; D010084:Oxidation-Reduction
D001798:Blood Proteins; D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated; D009227:Myristic Acids; D019814:Myristic Acid; D016718:Arachidonic Acid; D015118:Eicosapentaenoic Acid