title: Aggregation of phospholipid vesicles induced by the ribosome inactivating protein saporin.
abstract: Saporin-S6(SO-6) is a single chain ribosome inactivating protein, which can inhibit protein synthesis by inactivating eukaryotic ribosomes. The interaction of SO-6 with phospholipid model systems was described. SO-6 can specifically interact with negatively-charged phospholipid vesicles and it induces the aggregation of the lipid vesicles. The kinetics of the vesicle aggregation induced by SO-6 was studied. The saturating protein/lipid molar ratio was determined to be 1:100 based on titration experiments. The aggregation is dependent on the temperature in a range that was many times higher than the phase transition temperature of the phospholipid. The effect of pH on the aggregation of the vesicles can not be explained by simple deprotonation of side chain amino groups of the protein, and may be related to conformational changes of the protein. The maintenance of physiological ionic strength was required for the aggregation of SO-6 with vesicles. Finally, the interaction was prompted by Ca2+ ions, and was totally inhibited by EDTA, which suggests that SO-6 may interact with phospholipid vesicles in a Ca(2+)-dependent manner.
authors.affiliation: Department of Molecular Biology, Jilin University, Changchun, China.
authors.forename: Q
authors.initials: Q
authors.lastname: Hao
authors.forename: L
authors.initials: L
authors.lastname: Yan
authors.forename: H
authors.initials: H
authors.lastname: Yang
authors.forename: Y
authors.initials: Y
authors.lastname: Zhang
authors.forename: G
authors.initials: G
authors.lastname: Gao
authors.forename: Q
authors.initials: Q
authors.lastname: Yao
authors.forename: Q
authors.initials: Q
authors.lastname: Li
mesh_terms: D002118:Calcium / Q000494:pharmacology; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004492:Edetic Acid / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D000922:Immunotoxins; D007700:Kinetics; D008081:Liposomes / Q000737:chemistry*; D009699:N-Glycosyl Hydrolases; D009994:Osmolar Concentration; D010715:Phosphatidylglycerols / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry*; D010940:Plant Proteins / Q000737:chemistry* / Q000494:pharmacology; D054789:Ribosome Inactivating Proteins, Type 1; D000076985:Saporins; D013053:Spectrophotometry; D013696:Temperature
chemical_list: D000922:Immunotoxins; D008081:Liposomes; D010715:Phosphatidylglycerols; D010743:Phospholipids; D010940:Plant Proteins; D054789:Ribosome Inactivating Proteins, Type 1; D004492:Edetic Acid; C002773:dimyristoylphosphatidylglycerol; D009699:N-Glycosyl Hydrolases; D000076985:Saporins; D002118:Calcium