Effects of aluminum(III) on catechol-stimulated nerve growth factor biosynthesis by cultured mouse brain astroglial cells.
The effects of aluminum and other metal ions on the synthesis of nerve growth factor (NGF) by cultured mouse brain astroglial cells have been investigated. Natural NGF formation was dependent on the AlCl3 concentration of the cell culture medium; it was stimulated at low concentrations but was inhibited at higher concentrations. Catechol-stimulated NGF formation was also inhibited at AlCl3/catechol molar ratios > 0.3, whereas ZnCl2 and CaCl2 had no effect under similar conditions. Ethylenediamine-N,N,N',N'-tetraacetate (EDTA) and citrate blocked the inhibitory effect of Al(III). These observations were explained by the complex formation between Al(III) and catechols.
Kobayashi
D
D
Department of Chemistry, Faculty of Science, Nagoya University, Japan.
Murase
K
K
Odani
A
A
Hayashi
K
K
Yamauchi
O
O
D000535:Aluminum / Q000633:toxicity*; D000818:Animals; D001253:Astrocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D002396:Catechols / Q000494:pharmacology*; D002478:Cells, Cultured; D004347:Drug Interactions; D007124:Immunoenzyme Techniques; D051379:Mice; D009414:Nerve Growth Factors / Q000096:biosynthesis*; D012996:Solutions; D013268:Stimulation, Chemical
D002396:Catechols; D009414:Nerve Growth Factors; D012996:Solutions; D000535:Aluminum; C034221:catechol