Characterization of a new peptide from Tityus serrulatus scorpion venom which is a ligand of the apamin-binding site.
A new ligand (Ts kappa) of the apamin binding site on rat brain synaptosomes (K0.5 = 300 pM) was purified and characterized from the venom of Tityus serrulatus. It is a polypeptide toxin of 35 amino acid residues, with three disulfide bridges. Its cDNA was amplified from a venom gland cDNA library and the nucleotide sequence determined. A model of Ts kappa was constructed by amino acid replacement using charybdotoxin structure as determined by 1H nuclear magnetic resonance as starting model.
Legros
C
C
Laboratoire de Biochimie, URA 1455 du Centre National de la Recherche Scientifique, Institut Fédératif Jean Roche, Faculté de Médecine Nord, Marseille, France.
Oughuideni
R
R
Darbon
H
H
Rochat
H
H
Bougis
P E
PE
Martin-Eauclaire
M F
MF
D000595:Amino Acid Sequence; D000818:Animals; D001030:Apamin / Q000378:metabolism; D001483:Base Sequence; D001921:Brain / Q000378:metabolism*; D017931:DNA Primers; D018076:DNA, Complementary; D008024:Ligands; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009498:Neurotoxins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D016133:Polymerase Chain Reaction; D015221:Potassium Channels / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology*; D011487:Protein Conformation; D051381:Rats; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D012604:Scorpion Venoms / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D012605:Scorpions; D017386:Sequence Homology, Amino Acid; D013574:Synaptosomes / Q000378:metabolism*
D017931:DNA Primers; D018076:DNA, Complementary; D008024:Ligands; D009498:Neurotoxins; D010455:Peptides; D015221:Potassium Channels; D011994:Recombinant Proteins; D012604:Scorpion Venoms; C033799:apamin receptor; D001030:Apamin; C003609:tityustoxin