Reductase and oxygenase activities of semisynthetic flavocytochromes based on cytochrome P450 2B4.
A synthetic flavocytochrome with the reductase and oxygenase activities was obtained by covalent binding of riboflavin to cytochrome P450 2B4. The reactions catalyzed by the newly synthesized flavocytochromes were studied. Formation of carboxycomplex with the reduced form of hemoprotein led to 60-80% inhibition of oxygenase reactions indicating the leading role of reduced heme iron in generating active oxygen species by flavocytochromes.
Shumyantseva
V V
VV
Institute of Biomedical Chemistry, Moscow, Russia.
Uvarov
V Y
VY
Byakova
O E
OE
Archakov
A I
AI
D000814:Aniline Compounds / Q000378:metabolism; D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D003574:Cytochrome c Group / Q000378:metabolism; D005292:Ferricyanides / Q000378:metabolism; D006900:Hydroxylation; D008297:Male; D008745:Methylation; D009243:NAD / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D010088:Oxidoreductases / Q000378:metabolism; D010105:Oxygenases / Q000378:metabolism; D011817:Rabbits; D012256:Riboflavin / Q000378:metabolism*; D013053:Spectrophotometry; D013250:Steroid Hydroxylases / Q000378:metabolism*
D000814:Aniline Compounds; D003574:Cytochrome c Group; D005292:Ferricyanides; D009243:NAD; C007931:hexacyanoferrate III; D009249:NADP; C015157:N,N-dimethylaniline; D003577:Cytochrome P-450 Enzyme System; D010088:Oxidoreductases; D010105:Oxygenases; D013250:Steroid Hydroxylases; D001189:Aryl Hydrocarbon Hydroxylases; C050356:steroid 15-alpha-hydroxylase; C023650:aniline; D012256:Riboflavin