title: Regulation by nerve growth factor of neuropeptide phenotypes in primary cultured sensory neurons prepared from aged as well as adult mice.
abstract: The present study investigated neuropeptide phenotypes of aged, as well as adult, mouse sensory neurons. Proportions of somatostatin (SOM), calcitonin gene related protein (CGRP) and neuropeptide Y (NPY) immunoreactive (ir)-neurons were lower in primary cultures from aged (2 years) mice than in those from adult (6 months) animals, but similar for substance P (SP) in the absence of exogenous nerve growth factor (NGF). Addition of NGF, significantly enhanced (P < 0.05) proportions of SP, NPY and CGRP ir-neurons in both adult and aged cultures, whereas SOM ir-neurons were not affected in either. Thus SP, CGRP, NPY and SOM phenotypes are retained in cultured aged DRG neurons and some phenotypes can remain sensitive to NGF regulation.
authors.affiliation: Laboratory of Human Anatomy, University of Glasgow, Scotland, UK.
authors.forename: Z G
authors.initials: ZG
authors.lastname: Jiang
authors.forename: R A
authors.initials: RA
authors.lastname: Smith
mesh_terms: D000375:Aging / Q000378:metabolism*; D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000032:analysis; D002478:Cells, Cultured; D007124:Immunoenzyme Techniques; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D009414:Nerve Growth Factors / Q000502:physiology*; D009475:Neurons, Afferent / Q000737:chemistry*; D009478:Neuropeptide Y / Q000032:analysis; D009479:Neuropeptides / Q000032:analysis*; D010641:Phenotype; D013004:Somatostatin / Q000032:analysis; D013373:Substance P / Q000032:analysis
chemical_list: D009414:Nerve Growth Factors; D009478:Neuropeptide Y; D009479:Neuropeptides; D013373:Substance P; D013004:Somatostatin; D015740:Calcitonin Gene-Related Peptide