Caprine and bovine B rotaviruses in western France: group identification by Northern hybridization.
In a survey of coronavirus and rotavirus-induced neonatal diarrhoea in 373 calves from 284 farms, nine (2.4%) of the animals were found to be infected with non-group A rotaviruses when their faeces were analysed in a commercial ELISA and by PAGE. Seven out of eight 1-4- day-old kids from a single farm were also infected with similar viruses. In a mini-PAGE, all the viruses displayed group B- and/or E-like 4223 electrophoretypes. In Northern hybridizations with cDNA chemiluminescent probes specific to rotavirus groups A (RF strain), B (adult diarrhoea rotavirus, ADRV) and C (Cowden strain) all the viruses belonged to group B.
Gueguen
C
C
Laboratoire vétérinaire départemental, Rennes, France.
Maga
A
A
McCrae
M A
MA
Bataillon
G
G
D000818:Animals; D000831:Animals, Newborn; D001483:Base Sequence; D015152:Blotting, Northern; D002417:Cattle; D002418:Cattle Diseases; D017931:DNA Primers; D015342:DNA Probes; D018076:DNA, Complementary; D003967:Diarrhea / Q000662:veterinary* / Q000821:virology; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005602:France; D015511:Goat Diseases; D006041:Goats; D008163:Luminescent Measurements; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary; D012367:RNA, Viral / Q000032:analysis; D012401:Rotavirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D012400:Rotavirus Infections / Q000662:veterinary* / Q000821:virology
D017931:DNA Primers; D015342:DNA Probes; D018076:DNA, Complementary; D015345:Oligonucleotide Probes; D012367:RNA, Viral