Detection of circulating Trichinella pseudospiralis antigen by enzyme linked immunosorbant assay (ELISA).
A modified double antibody sandwich ELISA was used as a sensitive technique for demonstrating the appearance of circulating antigen of T. pseudospiralis in the sera of infected mice. The sera were tested at 5 days, 2, 4, 6, 7, 8, 10 and 12 weeks P.I. The antigen was first detected on the 5th day P.I., its level increased to reach the 1st peak at 4 weeks, then declined at 6 weeks. A second peak was observed at 8th weeks followed by a sharp decline to give negative OD reading at 10th week and increased to a moderate level at 12 weeks P.I.
Hegazy
I H
IH
Department of Parasitology, University of Alexandria, Egypt.
El Mansoury
S T
ST
Boulos
L M
LM
D000818:Animals; D000947:Antigens, Helminth / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D008297:Male; D051379:Mice; D011817:Rabbits; D012680:Sensitivity and Specificity; D013997:Time Factors; D014234:Trichinella / Q000276:immunology*; D014235:Trichinellosis / Q000097:blood / Q000175:diagnosis*
D000947:Antigens, Helminth