[Bacteriolytic enzyme preparation lysoamidase. Purification and some properties of bacteriolytic peptidase L1].
The bacteriolytic peptidase L1 has been isolated from the enzyme preparation of lysoamidase capable to lyze cell walls of gram-positive bacteria using ion-exchange chromatography and gel filtration. Some physico-chemical properties of the enzyme have been established. The molecular mass of L1 is 21 kDa, the pH optimum for Staphylococcus aureus cell lysis is 7-11. The optimal concentration of the buffer is 50 mM; the temperature optimum is 70 degrees C; the half-inactivation temperature is 55 degrees C.
Stepanaia
O A
OA
Begunova
E A
EA
Tsfasman
I M
IM
Kulaev
I S
IS
D002473:Cell Wall / Q000378:metabolism; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D010447:Peptide Hydrolases / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D013211:Staphylococcus aureus / Q000378:metabolism; D013696:Temperature
C060332:lysoamidase; D010447:Peptide Hydrolases; C101476:peptidase L1
Bakterioliticheskiĭ fermentnyĭ preparat lizoamidaza. Ochistka i nekotorye svoĭstva bakteriolithceskoĭ peptidazy L1.