p21WAF1/CIP1 interacts with protein kinase CK2.
p21WAF1/CIP1 which belongs to a class of regulatory proteins that interact with cyclin dependent kinases is a potent inhibitor of these kinases. The inhibition of the cyclin dependent kinases induces an arrest of cells in the G phase of the cell cycle. In addition p21WAF1/CIP1 associates with PCNA and inhibits DNA replication. Here, we show that p21WAF1/CIP1 binds to the regulatory beta-subunit of protein kinase CK2 but not to the catalytic alpha-subunit. Binding of p21WAF1/CIP1 down regulates the kinase activity of CK2 with respect to the phosphorylation of the beta-subunit of CK2, casein and the C-terminus of p53. This study demonstrates a new binding partner for the regulatory beta-subunit of protein kinase CK2 which regulates the activity of the holoenzyme.
Götz
C
C
Institute of Medical Biochemistry, University of the Saarland, Homburg, Germany.
Wagner
P
P
Issinger
O G
OG
Montenarh
M
M
D000595:Amino Acid Sequence; D001667:Binding, Competitive; D047390:Casein Kinase II; D050759:Cyclin-Dependent Kinase Inhibitor p21; D016213:Cyclins / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology*; D018076:DNA, Complementary / Q000235:genetics; D006801:Humans; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism; D010766:Phosphorylation; D017346:Protein Serine-Threonine Kinases / Q000037:antagonists & inhibitors* / Q000302:isolation & purification / Q000378:metabolism*
C493956:CDKN1A protein, human; D050759:Cyclin-Dependent Kinase Inhibitor p21; D016213:Cyclins; D018076:DNA, Complementary; D046911:Macromolecular Substances; D047390:Casein Kinase II; D017346:Protein Serine-Threonine Kinases; D010744:Phosphoric Monoester Hydrolases