title: Disposition of valpromide, valproic acid, and valnoctamide in the brain, liver, plasma, and urine of rats.
abstract: Valpromide (VPD) and valnoctamide (VCD) are amide derivatives of valproic acid (VPA), one of the major antiepileptic drugs (AEDs). In rodent models, both VPD and VCD are more potent as anticonvulsants than VPA. However, in humans, VPD served as a prodrug to VPA, whereas VCD acts as a drug on its own, which is not biotransformed to its corresponding acid--valnoctic acid (VCA). The present study investigates the pharmacokinetics (PKs of VPD and VCD in rats by monitoring the levels of these two amide isomers in the brain, liver, plasma, and urine of rats. The disposition of VPD and VCD was analyzed in a comparative manner with that of VPA. The following PK parameters were obtained for VPD and VCD, respectively: clearance, 6.1 and 3 ml/min/kg; volume of distribution (Vss), 0.63 and 0.58 liter/kg; half-life (t1/2), 42 and 94 min; and mean residence time (MRT), 102 and 196 min. The clearance of VCD in rats was half of that of VPD, and their Vss was similar. Therefore, VCD, t1/2, and MRT were twice as long as those of VPD.PK analysis of VPD and VCD liver and brain levels gave similar major PK parameters to those obtained from the plasma data. VPD underwent hepatic biotransformation to VPA, which persisted in the liver and brain for a longer period than VPD. The fraction metabolized of VPD to VPA was 42%. The brain was not found to be a metabolic site of the VPD-VPA biotransformation. Unlike VPD, VCD did not undergo amid-acid biotransformation to its corresponding acid, but was eliminated by biotransformation to unidentified metabolites. In contrast to VPD and VCD that distributed about evenly between the plasma, liver, and brain, VPA showed different disposition patterns in the plasma, liver, and brain. VCD and VPD distribute better into the brain than VPA, a fact that may contribute to their better anticonvulsant activity.
authors.affiliation: Department of Pharmaceutics, Faculty of Medicine, Hebrew University of Jerusalem, Israel.
authors.forename: S
authors.initials: S
authors.lastname: Blotnik
authors.forename: F
authors.initials: F
authors.lastname: Bergman
authors.forename: M
authors.initials: M
authors.lastname: Bialer
mesh_terms: D000577:Amides / Q000097:blood / Q000493:pharmacokinetics* / Q000652:urine; D000818:Animals; D014151:Anti-Anxiety Agents / Q000493:pharmacokinetics; D000927:Anticonvulsants / Q000493:pharmacokinetics*; D001921:Brain / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008297:Male; D051381:Rats; D014018:Tissue Distribution; D014635:Valproic Acid / Q000031:analogs & derivatives* / Q000097:blood / Q000493:pharmacokinetics* / Q000652:urine
chemical_list: D000577:Amides; D014151:Anti-Anxiety Agents; D000927:Anticonvulsants; C045179:valnoctamide; D014635:Valproic Acid; C004651:dipropylacetamide