title: Blood stem cell collection: factors influencing the recovery of granulocyte-macrophage colony forming cells.
abstract: We evaluated data from all blood cell (BC) collections performed in our institution between 1989 and 1995 to determine factors influencing the outcome of collection. One hundred and thirty-three collections were performed on 106 patients. Malignant diagnoses were: non-Hodgkins lymphoma (NHL) in 35%, multiple myeloma in 31%, breast cancer in 26%, and Hodgkin's disease in 8%. Collections were obtained routinely in myeloma and breast cancer and due to bone marrow involvement with malignancy or inaspirable bone marrow in lymphoma patients. Collections were obtained on a Cobe Spectra or Baxter-Fenwall CS3000+. Engraftment potential was determined by methylcellulose colony assay (CFU-GM), with a target of > 10 x 10(4) CFU-GM/kg. Apheresis nucleated cell count correlated significantly, albeit weakly (r = 0.26), with CFU-GM with a cell count of > 5 x 10(8)/kg resulting in an adequate number of CFU-GM in 78% of patients. In univariant analysis outcome of collection was significantly influenced by the patients age (p = 0.01), malignant diagnosis (p < 0.001), reason for collection (p = 0.002), and the mobilization regimen (p = 0.01). The nature of the apheresis device used did not influence outcome. Only malignant diagnosis was significant (p < 0.001) in multivariate analysis. We conclude that the outcome of BC is most strongly influenced by patient factors such as malignant diagnosis. These factors must be considered when comparing the outcome of different mobilization regimens and when planning collection strategies.
authors.affiliation: University of Toronto Autologous Bone Marrow Transplant Program, Toronto Hospital, (General Division), Ontario, Canada.
authors.forename: K
authors.initials: K
authors.lastname: Imrie
authors.forename: A K
authors.initials: AK
authors.lastname: Stewart
authors.forename: R M
authors.initials: RM
authors.lastname: Crump
authors.forename: H M
authors.initials: HM
authors.lastname: Prince
authors.forename: K
authors.initials: K
authors.lastname: Trip
authors.forename: A
authors.initials: A
authors.lastname: Keating
mesh_terms: D000293:Adolescent; D000328:Adult; D000704:Analysis of Variance; D001781:Blood Component Removal / Q000295:instrumentation / Q000379:methods*; D001853:Bone Marrow / Q000473:pathology; D001943:Breast Neoplasms / Q000097:blood / Q000628:therapy*; D003114:Colony-Forming Units Assay; D005260:Female; D006098:Granulocytes / Q000473:pathology*; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000473:pathology*; D006689:Hodgkin Disease / Q000097:blood / Q000628:therapy*; D006801:Humans; D008228:Lymphoma, Non-Hodgkin / Q000097:blood / Q000628:therapy*; D008264:Macrophages / Q000473:pathology*; D008875:Middle Aged; D009101:Multiple Myeloma / Q000097:blood / Q000628:therapy*; D015999:Multivariate Analysis; D012189:Retrospective Studies; D014182:Transplantation, Autologous; D016896:Treatment Outcome