title: Mosaic type of the nontoxic-nonhemaggulutinin component gene in Clostridium botulinum type A strain isolated from infant botulism in Japan.
abstract: The gene encoding the nontoxic-nonhemaggulutinin (NTNH) component was amplified by the PCR technique using two primer sets and the DNA template from Clostridium botulinum type A strain 7I03-H isolated from infant botulism in Japan. The nucleotide sequence revealed that the NTNH gene was composed of 1,193 amino acids with a molecular weight of 130868.08. Furthermore, the N-terminal half side and C-terminal half side of the NTNH component were similar to the NTNH component of type C and type A, respectively. These results indicate that the NTNH component gene codes the mosaic NTNH component composed of type A and type C. The hemaggulutinin gene, aha, and ORF-22 gene, orf-22a, were undetectable in the region upstream of the NTNH component gene, ant. Therefore, orf-22a is not thought to play a key role in the expression of botulinum type A progenitor toxin gene.
authors.affiliation: Department of Microbiology, Sapporo Medical University School of Medicine, Hokkaido, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Kubota
authors.forename: S
authors.initials: S
authors.lastname: Shirakawa
authors.forename: S
authors.initials: S
authors.lastname: Kozaki
authors.forename: E
authors.initials: E
authors.lastname: Isogai
authors.forename: H
authors.initials: H
authors.lastname: Isogai
authors.forename: K
authors.initials: K
authors.lastname: Kimura
authors.forename: N
authors.initials: N
authors.lastname: Fujii
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D001905:Botulinum Toxins / Q000633:toxicity*; D001906:Botulism / Q000382:microbiology*; D003001:Cloning, Molecular; D003014:Clostridium botulinum / Q000235:genetics* / Q000302:isolation & purification; D004269:DNA, Bacterial; D006801:Humans; D007231:Infant, Newborn; D008969:Molecular Sequence Data; D009030:Mosaicism; D009498:Neurotoxins / Q000633:toxicity*; D016366:Open Reading Frames; D017386:Sequence Homology, Amino Acid
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; C088300:NTNH protein, Clostridium botulinum; D009498:Neurotoxins; D001905:Botulinum Toxins