Pseudosubstrate flexibility in chicken smooth muscle myosin light chain kinase.
Chicken smooth muscle myosin light chain kinase pseudosubstrate is contained within the C-terminal region of the sequence MLCK(774-807)amide. It forms a helical conformation which interacts with the connecting peptide contained within the N-terminal portion of the sequence. These regions are linked by a well-defined turn around Ser-787 which supports a model of pseudosubstrate regulation involving the hinging of the pseudosubstrate or autoregulatory recognition sequence away from the active site of the catalytic core following the binding of calmodulin to the kinase.
Barden
J A
JA
Institute for Biomedical Research, University of Sydney, New South Wales, Australia.
Kemp
B E
BE
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002645:Chickens; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009130:Muscle, Smooth / Q000201:enzymology*; D009219:Myosin-Light-Chain Kinase / Q000737:chemistry / Q000378:metabolism*; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D012996:Solutions; D013379:Substrate Specificity
D010446:Peptide Fragments; D012996:Solutions; C100743:myosin light chain kinase (774-807) amide; D009219:Myosin-Light-Chain Kinase