Reevaluation of enzyme-linked immunosorbent assay with ultraviolet-treated polystyrene microtiter plates for measurement of antibodies to dsDNA.
We reevaluated the efficacy of enzyme-linked immunosorbent assay (ELISA) with ultraviolet (UV)-treated polystyrene microtiter plates (UV-ELISA) for the detection of human serum anti-dsDNA antibodies. The subjects consisted of 38 patients with systemic lupus erythematosus (SLE). The titers of IgG serum anti-dsDNA antibodies by UV-ELISA were significantly (p < 0.01) higher in sera from 26 active SLE patients than in those from 12 inactive SLE patients. ELISA with poly-L-lysine coated microtiter plates (PLL-ELISA) revealed the similar results. However, the background ratio was only 1.0 +/- 0.9% by UV-ELISA, which was significantly (p < 0.01) lower than that by PLL-ELISA (16.8 +/- 10.8%). These results demonstrated the efficacy of ELISA with UV-treated polystyrene microtiter plates for the measurement of human serum anti-dsDNA antibodies in patients with SLE.
Kaburaki
J
J
Department of Internal Medicine, Keio University School of Medicine.
Ogasawara
T
T
Hayakawa
M
M
Kuwana
M
M
Tojo
T
T
Ikeda
Y
Y
D000974:Antibodies, Antinuclear / Q000032:analysis*; D004247:DNA / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000276:immunology; D011137:Polystyrenes; D014466:Ultraviolet Rays
D000974:Antibodies, Antinuclear; D011137:Polystyrenes; D004247:DNA