title: [Preparation of 125I-F(ab')2-DNR-liposomes and radioimmunoassay of their specific conjugating ability].
abstract: The F(ab')2-DNR-liposomes were prepared using F(ab')2 obtained from digested monoclonal antibody against human white cells HI30 (CD45). Their affinity constant for peripheral blood mononuclear cells was determined by radioimmunoassay. Ka = 2.0 x 10(9). A good specific conjugation ability was demonstrated. In preparing the immunoliposomes, it was found that F(ab')2 coupled to the smaller liposomes with higher conjugation ratio.
authors.affiliation: Hematology Institute, CAMS and PUMC, Tianjin.
authors.forename: J
authors.initials: J
authors.lastname: Liu
authors.forename: Z
authors.initials: Z
authors.lastname: Chen
authors.forename: H
authors.initials: H
authors.lastname: Liu
authors.forename: Q
authors.initials: Q
authors.lastname: Fan
authors.forename: D
authors.initials: D
authors.lastname: Shen
mesh_terms: D000903:Antibiotics, Antineoplastic / Q000008:administration & dosage*; D000911:Antibodies, Monoclonal; D001667:Binding, Competitive; D003630:Daunorubicin / Q000008:administration & dosage*; D006801:Humans; D007140:Immunoglobulin Fab Fragments; D000922:Immunotoxins; D007457:Iodine Radioisotopes; D017493:Leukocyte Common Antigens / Q000276:immunology; D008081:Liposomes; D011863:Radioimmunoassay
chemical_list: D000903:Antibiotics, Antineoplastic; D000911:Antibodies, Monoclonal; D007140:Immunoglobulin Fab Fragments; D000922:Immunotoxins; D007457:Iodine Radioisotopes; D008081:Liposomes; D017493:Leukocyte Common Antigens; D003630:Daunorubicin