Are cytoskeletal components involved in the control of hepatic carnitine palmitoyltransferase I activity?
The present work was undertaken to test whether cytoskeletal components are involved in the control of rat-liver carnitine palmitoyltransferase I (CPT-I) activity by cellular effectors. The microtubule stabilizer taxol abolished the changes in CPT-I activity induced by the effectors tested. Taxol also prevented OA-induced shrinkage of hepatocytes as well as the enhanced release of lactate dehydrogenase from digitonin-permeabilized hepatocytes. On the basis of its relative sensitivity to tautomycin and OA, the modulation of CPT-I activity seemed to involve mostly protein phosphatase 1. These data suggest that the short-term control of hepatic CPT-I by cellular effectors may involve modulation of interactions between CPT-I and cytoskeletal components.
Velasco
G
G
Department of Biochemistry and Molecular Biology I, Faculty of Biology, Complutense University, Madrid, Spain.
Sánchez
C
C
Geelen
M J
MJ
Guzmán
M
M
D000818:Animals; D000935:Antifungal Agents / Q000494:pharmacology; D003994:Bucladesine / Q000494:pharmacology; D002334:Carnitine O-Palmitoyltransferase / Q000378:metabolism*; D002478:Cells, Cultured; D003599:Cytoskeleton / Q000187:drug effects / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004988:Ethers, Cyclic / Q000494:pharmacology; D005973:Glutamine / Q000494:pharmacology; D008099:Liver / Q000166:cytology / Q000201:enzymology*; D008297:Male; D008870:Microtubules / Q000187:drug effects; D019319:Okadaic Acid; D017239:Paclitaxel / Q000494:pharmacology; D010749:Phosphoprotein Phosphatases / Q000037:antagonists & inhibitors; D054645:Protein Phosphatase 1; D011714:Pyrans; D051381:Rats; D017208:Rats, Wistar; D013141:Spiro Compounds; D014638:Vanadates / Q000494:pharmacology
D000935:Antifungal Agents; D004791:Enzyme Inhibitors; D004988:Ethers, Cyclic; D011714:Pyrans; D013141:Spiro Compounds; D005973:Glutamine; C053079:tautomycin; D019319:Okadaic Acid; D014638:Vanadates; D003994:Bucladesine; D002334:Carnitine O-Palmitoyltransferase; D010749:Phosphoprotein Phosphatases; D054645:Protein Phosphatase 1; D017239:Paclitaxel