title: A proposal for the conformation of loop E in Escherichia coli 5S rRNA.
abstract: A proposal is advanced for the conformation of the loop E region of prokaryotic 5S rRNAs based on spectroscopic data obtained from pAD3 RNA, a construct that includes helix IV, helix V, and loops D and E from Escherichia coli 5S rRNA. Even though loop E juxtaposes bases that cannot form Watson-Crick base pairs, it resembles an A-form double helix; its nucleotides relate to each other spectroscopically in a helix-like way and are in the anti conformation. The ends of loop E, which is palindromic, have the same conformation. Working in from either end towards the center of the loop, a closing GC is followed by a side-by-side GA and then by a reversed Hoogsteen AU, a pattern resembling that found at one end of eukaryotic loop E. The center of the loop consists of three nucleotide pairs, which appear to be an asymmetric GG pair, a Watson-Crick-like AG, and a GU stabilized by a single hydrogen bond.
authors.affiliation: Departments of Chemistry and Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520-8107, USA.
authors.forename: A
authors.initials: A
authors.lastname: Dallas
authors.forename: R
authors.initials: R
authors.lastname: Rycyna
authors.forename: P
authors.initials: P
authors.lastname: Moore
mesh_terms: D001483:Base Sequence; D004926:Escherichia coli / Q000235:genetics*; D006860:Hydrogen Bonding; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009690:Nucleic Acid Conformation; D011387:Prokaryotic Cells; D012329:RNA, Bacterial / Q000235:genetics*; D012341:RNA, Ribosomal, 5S / Q000235:genetics*
chemical_list: D012329:RNA, Bacterial; D012341:RNA, Ribosomal, 5S