Effects of oil adjuvant on systemic response to Escherichia coli lipopolysaccharide in swine.
Intramuscular injection of 0.1 mg/kg of Escherichia coli lipopolysaccharide (LPS) mixed with Freund's complete adjuvant (LPS+FCA) in piglets mitigated the leukopenia and TNF-alpha and cortisol levels in the serum compared with that of LPS suspended in LPS-free saline. The endotoxin level in the serum of the LPS+FCA was remarkably reduced. These results suggest that the addition of oil adjuvant mitigate the systemic toxicity of LPS.
Norimatsu
M
M
National Veterinary Assay Laboratory, Kokubunji, Tokyo.
Ogikubo
Y
Y
Kojima
A
A
Takahashi
T
T
Watanabe
G
G
Taya
K
K
Sasamoto
S
S
Tamura
Y
Y
D000818:Animals; D001428:Bacterial Vaccines; D004926:Escherichia coli / Q000276:immunology*; D004927:Escherichia coli Infections / Q000276:immunology / Q000517:prevention & control*; D005620:Freund's Adjuvant; D006854:Hydrocortisone / Q000097:blood; D007273:Injections, Intramuscular; D007970:Leukopenia / Q000517:prevention & control*; D008070:Lipopolysaccharides / Q000008:administration & dosage* / Q000276:immunology / Q000633:toxicity; D009821:Oils; D012965:Sodium Chloride; D013552:Swine; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D001428:Bacterial Vaccines; D008070:Lipopolysaccharides; D009821:Oils; D014409:Tumor Necrosis Factor-alpha; D012965:Sodium Chloride; D005620:Freund's Adjuvant; D006854:Hydrocortisone