[The reversing effect of HPV-16 E6E7 gene antisense plasmid on the HPV-16 positive human cervical carcinoma cells].
Antisense reconstruct, p16as E6E7Neo, harboring HPV-16E6E7 gene of which the expression is regulatable by dexamethasone was constructed. Calcium phosphate mediated transfection was employed to transduce this antisense reconstruct into HPV-16 positive CasKi cell line and HPV negative C-33A cell line (both derived from human cervical carcinoma) respectively. After dexamethasone was added into the culture medium to induce the expression of E6E7 antisense gene, the malignity of CasKi cells were reversed, while the growth characteristics and malignity of C-33A cells were unchanged. The results show that the reversing effect on CasKi cell is specific and mediated by inhibition of expression of E6E7 gene. It also demonstrated a more profound pathogenic role of HPV-16 E6E7 gene in the tumorogenesis of cervical carcinoma and provides a possibility for gene therapy of this tumor.
Zeng
P
P
Department of Biophysics, Institute of Basic Medical Sciences, CAMS, Beijing.
Si
J
J
Guo
R
R
D000818:Animals; D018931:Antineoplastic Agents, Hormonal / Q000494:pharmacology; D002471:Cell Transformation, Neoplastic / Q000187:drug effects*; D003907:Dexamethasone / Q000494:pharmacology; D005260:Female; D015967:Gene Expression Regulation, Viral / Q000187:drug effects; D005814:Genes, Viral; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D027383:Papillomaviridae / Q000235:genetics*; D016372:RNA, Antisense / Q000235:genetics / Q000494:pharmacology*; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000821:virology
D018931:Antineoplastic Agents, Hormonal; D016372:RNA, Antisense; D003907:Dexamethasone