Ultrastructural investigation of lead-induced intranuclear inclusion bodies in mice.
By means of optical and electron microscopy and by atomic absorption spectrophotometry in a graphite chamber, this study evaluated the effect of temperature (22-35 degrees C) on lesions in the kidney, liver, and brain, and on concentrations of lead caused by the administration of 2 and 5 mg/kg/IP of lead acetate to Swiss mice. The most pronounced effects were observed in the kidney and in groups of animals receiving the highest doses (5 mg/kg at 22 and 35 degrees C). These effects consisted of significantly higher (p < .05) lead concentrations in the tissues, a significant decrease (p < .05) in kidney weights, and progressive kidney atrophy and fibrosis with, at the ultrastructural level, constant intranuclear inclusions, which were also observed in the cytoplasm of renal and endothelial cells.
Vicente-Ortega
V
V
Department of Pathology, Faculty of Medicine, University of Murcia, Spain.
Martínez-García
A F
AF
Cremades-Campos
A
A
Rodríguez-Vicente
J
J
Calderón-Rubiales
F
F
Martínez-Díaz
F
F
D000821:Animal Feed / Q000633:toxicity; D000818:Animals; D001921:Brain / Q000187:drug effects / Q000473:pathology / Q000648:ultrastructure; D001923:Brain Chemistry / Q000187:drug effects; D002467:Cell Nucleus / Q000737:chemistry / Q000187:drug effects* / Q000648:ultrastructure*; D002479:Inclusion Bodies / Q000737:chemistry / Q000187:drug effects* / Q000648:ultrastructure*; D007668:Kidney / Q000187:drug effects / Q000473:pathology / Q000648:ultrastructure; D007854:Lead / Q000032:analysis / Q000633:toxicity; D008099:Liver / Q000187:drug effects / Q000473:pathology / Q000648:ultrastructure; D008297:Male; D051379:Mice; D009942:Organometallic Compounds / Q000008:administration & dosage / Q000633:toxicity*; D013696:Temperature
D009942:Organometallic Compounds; D007854:Lead; C008261:lead acetate