title: Direct chiral resolution of 15-deoxyspergualin using a cellobiohydrolase liquid chromatographic column.
abstract: An LC method for the direct determination of the enantiomers of 15-deoxyspergualin was developed, optimized and validated. A commercially available LC column containing a cellulase enzyme (cellobiohydrolase I) stationary phase was used. The effect of various parameters on the separation and validation data are discussed.
authors.affiliation: Bristol-Myers Squibb Pharmaceutical Research Institute, New Brunswick, NJ 08903-0191, USA.
authors.forename: D J
authors.initials: DJ
authors.lastname: Mayo
authors.forename: F P
authors.initials: FP
authors.lastname: Tomasella
authors.forename: J E
authors.initials: JE
authors.lastname: Noroski
mesh_terms: D002480:Cellulase / Q000032:analysis; D043366:Cellulose 1,4-beta-Cellobiosidase; D002853:Chromatography, Liquid / Q000379:methods*; D006146:Guanidines / Q000032:analysis* / Q000737:chemistry; D007166:Immunosuppressive Agents / Q000032:analysis* / Q000737:chemistry; D008968:Molecular Conformation
chemical_list: D006146:Guanidines; D007166:Immunosuppressive Agents; D002480:Cellulase; D043366:Cellulose 1,4-beta-Cellobiosidase; C037258:gusperimus