A new method of superoxide free radical determination in ischemia and reperfusion injury of rat liver.
The present study was designed to utilize the chemiluminescence (CL) method for O2- analysis in ischemia and reperfusion of the whole rat liver. The results indicated that the O2- level was lower after 30 and 60 min ischemia. After 40 min reperfusion, the level of O2- increased obviously, reaching about 8.3-fold (30 min ischemia) and 9.0-fold (60 min ischemia) (P < 0.001, P < 0.01) respectively. The results also show that Chinese Traditional Medicines ligustrazine and salvia compound can scavenge O2- effectively.
Wu
W G
WG
Department of Surgery, Tongji Hospital, Tongji Medical University, Wuhan.
Qiu
F Z
FZ
D000818:Animals; D005609:Free Radicals / Q000032:analysis; D007511:Ischemia / Q000378:metabolism*; D008099:Liver / Q000098:blood supply*; D008163:Luminescent Measurements; D008297:Male; D010100:Oxygen / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000378:metabolism*
D005609:Free Radicals; D010100:Oxygen