title: Mapping the human Y chromosome by fingerprinting cosmid clones.
abstract: We have used Y-specific cosmid clones in a random fingerprinting approach to build contigs on the human Y chromosome. Clones derived from two libraries have been analyzed. The construction of one library is described here, the second was the Y chromosome-specific library LLOYNCO3 "M" (Lawrence Livermore National Laboratory). To date, we have fingerprinted 4430 cosmids: 377 contigs have been constructed containing from 2 to 39 clones. Along with the singletons, we estimate that we have covered 72.5% of the euchomatic portion of the Y chromosome with fingerprinted clones. Sequence tagged sites are being used to anchor cosmids and contigs onto the YAC framework.
authors.affiliation: Galton Laboratory, University College London, UK. k.taylor@genetics.ucl.ac.uk
authors.forename: K
authors.initials: K
authors.lastname: Taylor
authors.forename: N
authors.initials: N
authors.lastname: Hornigold
authors.forename: D
authors.initials: D
authors.lastname: Conway
authors.forename: D
authors.initials: D
authors.lastname: Williams
authors.forename: Z
authors.initials: Z
authors.lastname: Ulinowski
authors.forename: M
authors.initials: M
authors.lastname: Agochiya
authors.forename: P
authors.initials: P
authors.lastname: Fattorini
authors.forename: P
authors.initials: P
authors.lastname: de Jong
authors.forename: P F
authors.initials: PF
authors.lastname: Little
authors.forename: J
authors.initials: J
authors.lastname: Wolfe
mesh_terms: D001483:Base Sequence; D002874:Chromosome Mapping / Q000379:methods*; D003001:Cloning, Molecular; D003360:Cosmids; D016172:DNA Fingerprinting; D017931:DNA Primers; D006801:Humans; D008969:Molecular Sequence Data; D014998:Y Chromosome
chemical_list: D017931:DNA Primers