Expression of Na+/myo-inositol cotransporter mRNA in normal and hypertonic stress rat eyes.
We studied the localization of Na+/myo-inositol cotransporter (SMIT) mRNA in normal and hypertonic stress rat eyes by in situ hybridization histochemistry using cRNA probes. SMIT mRNA signals were observed in the iris-ciliary body, the lens epithelial cells, and the ganglion cell layer and the inner nuclear layer of the retina. There was a rapid increase on SMIT mRNA in the retina of hypertonic stress rats compared with control rats. These findings suggest that Na+/myo-inositol cotransporter gene expression is osmotically regulated in vivo to protect retinal neuronal function against hypertonic stress.
Morimura
H
H
Department of Ophthalmology, Osaka University Medical School, Japan.
Shimada
S
S
Otori
Y
Y
Yamauchi
A
A
Minami
Y
Y
Inoue
K
K
Miyai
A
A
Ishimoto
I
I
Tano
Y
Y
Tohyama
M
M
D000818:Animals; D002352:Carrier Proteins / Q000096:biosynthesis*; D002924:Ciliary Body / Q000378:metabolism; D005123:Eye / Q000378:metabolism*; D015870:Gene Expression; D006360:Heat-Shock Proteins / Q000096:biosynthesis*; D017403:In Situ Hybridization; D007294:Inositol / Q000378:metabolism; D007908:Lens, Crystalline / Q000378:metabolism; D008297:Male; D008565:Membrane Proteins; D015347:RNA Probes; D018075:RNA, Complementary; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar; D012016:Reference Values; D012160:Retina / Q000378:metabolism; D012165:Retinal Ganglion Cells / Q000378:metabolism; D012462:Saline Solution, Hypertonic; D012964:Sodium / Q000378:metabolism; D013312:Stress, Physiological; D027981:Symporters
D002352:Carrier Proteins; D006360:Heat-Shock Proteins; D008565:Membrane Proteins; D015347:RNA Probes; D018075:RNA, Complementary; D012333:RNA, Messenger; D012462:Saline Solution, Hypertonic; D027981:Symporters; C073884:SLC5A3 protein, human; D007294:Inositol; D012964:Sodium