Morphological features and kinetics of primary cultures of BPH tissue supported by TV1 medium.
222 human prostatic biopsies were used to prepare cell cultures by means of a medium--colony formation permissive--containing fetal calf serum, called TV1. After 7, 14 and 21 days, the cultures were examined by optical and scanning electron microscopy. TV1 medium induces the formation and growth of two types of colonies, one mainly composed of epithelioid cells and distinguished by early growth; the second one made up exclusively of fibroblastoid cells which appear later in the culture. Epithelioid colonies, comprising three different cell types, appear to be arranged as a growth halo concentric to the bioptic fragment with a large central area, formed by a monolayer, and a pluristratified edge. Fibroblastoid cells weakly adhere to the substrate and form "satellite growth halos" separated from the primitive bioptic fragment. All the epithelioid cells were positive to cytokeratin LP34 Mab and negative to anti-smooth muscle-actin and anti-proline-4-hydroxylase antibodies. Fibroblastoid cells were only anti-proline-4-hydroxylase positive. The cell kinetics of epithelioid cells were also studied, revealing an extension of the S phase, in contrast to what happened with WAJC 404, and consequently a reduction of the percentage of cells entering mitosis. For this reason, the addition of fetal serum to the culture medium does not allow the use of prostate primary cultures for more than 14 days.
De Angeli
S
S
Laboratorio Colture Cellulari del Centro Trasfusionale, Piazzale dell'Ospedale, Padova, Italy.
Valenti
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F
Buoro
S
S
Conconi
M T
MT
Parnigotto
P P
PP
D000199:Actins / Q000032:analysis; D000368:Aged; D000369:Aged, 80 and over; D000911:Antibodies, Monoclonal; D001706:Biopsy; D002453:Cell Cycle; D002455:Cell Division; D002478:Cells, Cultured; D003470:Culture Media; D046508:Culture Techniques / Q000379:methods; D006801:Humans; D007633:Keratins / Q000032:analysis; D007700:Kinetics; D008297:Male; D008855:Microscopy, Electron, Scanning; D008875:Middle Aged; D011393:Procollagen-Proline Dioxygenase / Q000032:analysis; D011467:Prostate / Q000473:pathology* / Q000648:ultrastructure; D011470:Prostatic Hyperplasia / Q000473:pathology*
D000199:Actins; D000911:Antibodies, Monoclonal; D003470:Culture Media; D007633:Keratins; D011393:Procollagen-Proline Dioxygenase