title: PTX-sensitive regulation of neurite outgrowth by the dopamine D3 receptor.
abstract: Neurotransmitter receptors are known to have direct roles in the modulation of neuronal morphogenesis. Previous work showed that clonal mesencephalic MN9D cells individually transfected with D2-like dopamine receptors show increased neurite outgrowth following long-term exposure to the D2-like receptor agonist quinpirole. In the current study, brief stimulation of D3 receptor-expressing cells also elicited increased neurite outgrowth, which could be mimicked by the Gi/G(o) protein activator mastoparan. Pretreatment with the Gi/G(o) protein inhibitor pertussis-toxin blocked the quinpirole- and mastoparan-mediated increases in outgrowth. These results suggest that dopamine D3 receptor stimulation has an immediate, G-protein-mediated role in neuronal morphogenesis.
authors.affiliation: Department of Psychiatry, Washington University School of Medicine, St. Louis, MO 63110, USA.
authors.forename: B C
authors.initials: BC
authors.lastname: Swarzenski
authors.forename: K L
authors.initials: KL
authors.lastname: O'Malley
authors.forename: R D
authors.initials: RD
authors.lastname: Todd
mesh_terms: D000818:Animals; D002460:Cell Line; D018491:Dopamine Agonists / Q000494:pharmacology; D019204:GTP-Binding Proteins / Q000378:metabolism; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins; D016501:Neurites / Q000187:drug effects / Q000648:ultrastructure*; D010455:Peptides; D037342:Pertussis Toxin; D019257:Quinpirole / Q000037:antagonists & inhibitors / Q000494:pharmacology; D051381:Rats; D017448:Receptors, Dopamine D2 / Q000187:drug effects*; D050637:Receptors, Dopamine D3; D015398:Signal Transduction / Q000187:drug effects; D010566:Virulence Factors, Bordetella / Q000494:pharmacology*; D014862:Wasp Venoms / Q000037:antagonists & inhibitors / Q000494:pharmacology
chemical_list: C493593:DRD3 protein, human; D018491:Dopamine Agonists; C493595:Drd3 protein, rat; D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides; D017448:Receptors, Dopamine D2; D050637:Receptors, Dopamine D3; D010566:Virulence Factors, Bordetella; D014862:Wasp Venoms; D019257:Quinpirole; C037500:mastoparan; D037342:Pertussis Toxin; D019204:GTP-Binding Proteins