title: Influence of medium-chain triglyceride-based lipid emulsion on rat polymorphonuclear cell functions.
abstract: Intravenous lipid emulsions are used as energy and essential fatty acids sources. There are controversial reports postulating in vitro and in vivo inhibitory effects of long-chain triglycerides (LCT) upon the blood polymorphonuclear leukocytes (PMNL) functions. In the present study the in vivo and in vitro effects of LCT and a physical mixture of medium- and long-chain triglycerides (MCT/LCT) emulsions were investigated on select PMNL functions, i.e., chemotaxis, phagocytosis, and bacterial killing. Blood from 20 rats was incubated with LCT, MCT, MCT/LCT, and saline, respectively. MCT-containing emulsions exhibited an inhibitory effect on all PMNL functions investigated, whereas LCT exerted an effect on the phagocytic index only. The administration of a parenteral supply of LCT, MCT/LCT, and saline for 30 h followed by saline infusion for 14 h in discontinuous mode did not influence any of the investigated PMNL functions. Similarly, continuous infusion over 44 h at increasing infusion rates up to 1.5 mL/h did not affect the PMNL functions. The obvious difference between in vitro and in vivo response of the PMNL model emphasizes the necessity for continuous monitoring of in vivo conditions. Appropriate interpretation of the data requires continuous circumspection and consideration of trials in a clinical setting.
authors.affiliation: Department of Surgery, University of Sao Paulo Medical School, Brazil.
authors.forename: D L
authors.initials: DL
authors.lastname: Waitzberg
authors.forename: R
authors.initials: R
authors.lastname: Bellinati-Pires
authors.forename: N
authors.initials: N
authors.lastname: Yamaguchi
authors.forename: S
authors.initials: S
authors.lastname: Massili-Oku
authors.forename: M M
authors.initials: MM
authors.lastname: Salgado
authors.forename: I P
authors.initials: IP
authors.lastname: Hypolito
authors.forename: S C
authors.initials: SC
authors.lastname: Soares
authors.forename: E L
authors.initials: EL
authors.lastname: Goncalves
authors.forename: P
authors.initials: P
authors.lastname: Furst
mesh_terms: D000818:Animals; D001770:Blood Bactericidal Activity / Q000187:drug effects; D002633:Chemotaxis / Q000187:drug effects; D005217:Fat Emulsions, Intravenous / Q000008:administration & dosage / Q000494:pharmacology*; D008297:Male; D009504:Neutrophils / Q000187:drug effects* / Q000502:physiology*; D010587:Phagocytosis / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D014280:Triglycerides / Q000008:administration & dosage* / Q000494:pharmacology
chemical_list: D005217:Fat Emulsions, Intravenous; D014280:Triglycerides