[Study on skin storage at -20 degrees C].
Small pieces of fresh guinea pig or cadaveric skin, 0.3mm in thickness, were soaked in cryoprotective solution for 30 min, then were put into plastic bags and kept in -20 degrees C refrigerator. Before and after 14, 30, 45, 60, 75 days storage, the skin were sent for succinae dehydrogenase assay (modified Hershey's method) and oxygen consumption determination (microelectrolyte method). The average viabilities measured by succinated dehydrogenase and oxygen consumption were nearly 50% after 60 days storage and 30% after 75 days storage in both guinea pig and cadaveric skin. 37 times of autologous skin stored at -20 degrees C for 1-26 days were grafted on the granulation wounds with good results. Skin can be preserved viably at -20 degrees C for 45 days but not longer than 60 days. This method may be helpful for hospital which has no -80 degrees C deep freezer or liquid nitrogen container.
Zhu
Z
Z
304th Hospital, Pelope's Liberation Army, Beijing.
Chai
J
J
Kong
Q
Q
D000818:Animals; D002056:Burns / Q000601:surgery; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents; D006168:Guinea Pigs; D006801:Humans; D010101:Oxygen Consumption; D012867:Skin / Q000378:metabolism; D016038:Skin Transplantation; D013385:Succinate Dehydrogenase / Q000378:metabolism
D003451:Cryoprotective Agents; D013385:Succinate Dehydrogenase