Crystallization and preliminary X-ray analysis of human brain hexokinase.
Human brain hexokinase type I, expressed in Escherichia coli, has been crystallized from polyethylene glycol 8000 in the presence of inorganic phosphate. The crystals are hexagonal needles of diameter 0.25 mm, diffracting to a resolution of 3.5 A on a rotating-anode/area-detector system. The crystals belong to the space group P3(1)21/P3(2)21 with cell dimensions a = b = 171.5 A and c = 99.4 A. The specific volume of the crystal is 4.2 A3/Da, suggesting an asymmetric unit with a single 100 kDa molecule and a solvent content of 71% by volume or two molecules of hexokinase with a solvent content of 41%. The complex of hexokinase with glucose crystallizes under similar conditions, giving crystals of the same morphology.
Aleshin
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AE
Department of Biochemistry and Biophysics, Iowa State University, Ames 50011, USA.
Zeng
C
C
Fromm
H J
HJ
Honzatko
R B
RB
D001921:Brain / Q000201:enzymology*; D003460:Crystallization; D018360:Crystallography, X-Ray / Q000379:methods; D004926:Escherichia coli; D006593:Hexokinase / Q000096:biosynthesis / Q000737:chemistry*; D006801:Humans; D007527:Isoenzymes / Q000096:biosynthesis / Q000737:chemistry; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry
D007527:Isoenzymes; D011994:Recombinant Proteins; D006593:Hexokinase