An electrophysiological study of calcium entry during normal human T-lymphocyte activation.
Our aim was to observe whether normal human T-cells respond to mitogenic stimulation with large whole-cell inward currents (composed of identifiable single-channel contributions) when [Ca2+]i is not markedly lowered but instead kept normal or moderately low, as has been reported in human leukaemic Jurkat T-cell line and T-cell clones [Kuno et al. (1986) Nature 323, 269-73; Kuno and Gardner (1987) Nature 326, 301-304; Gardner (1990) Annu. Rev. Immunol. 8, 231-252]. Whole-cell patch recordings showed no such currents in cells otherwise normally responding to depolarisation with the macroscopic IK described in T-lymphocytes and thus deemed viable, in agreement with the notion that Ca2+ influx in normal T-cells enterily depends on depletion of internal stores [Putney (1986) Cell Calcium 7, 1-12; Putney (1990) Cell Calcium 11, 611-624].
Ricci
R
R
Istituto di Anatomia Patologica, Universita' Cattolica, Roma, Italy.
Buffelli
M
M
Riviera
A P
AP
Cangiano
A
A
D002118:Calcium / Q000378:metabolism*; D015220:Calcium Channels / Q000502:physiology*; D005071:Evoked Potentials; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D008213:Lymphocyte Activation; D008564:Membrane Potentials; D018408:Patch-Clamp Techniques; D010835:Phytohemagglutinins; D013601:T-Lymphocytes / Q000276:immunology / Q000502:physiology*; D013997:Time Factors; D014407:Tumor Cells, Cultured
D015220:Calcium Channels; D010835:Phytohemagglutinins; D002118:Calcium