Tranilast inhibits the effects of platelet-derived growth factor on cell proliferation and induction of nitric oxide.
The effects of tranilast on DNA synthesis and cell proliferation in cultured rat mesangial cells, treated with platelet-derived growth factor (PDGF), were investigated. Tranilast significantly inhibited PDGF-stimulated DNA synthesis and cell proliferation in a dose-dependent manner. In the absence of PDGF, it also enhanced cytokine-induced nitric oxide (NO) production, PDGF significantly inhibited cytokine-induced NO production, but tranilast completely abolished this inhibitory effect of PDGF. These results show that tranilast inhibits PDGF-induced proliferation of mesangial cells under both normal and inflammatory conditions.
Hishikawa
K
K
Department of Internal Medicine, Kcio University School of Medicine, Tokyo, Japan.
Nakaki
T
T
Hirahashi
J
J
Marumo
T
T
Saruta
T
T
D000818:Animals; D002455:Cell Division / Q000187:drug effects*; D002478:Cells, Cultured; D016207:Cytokines / Q000494:pharmacology; D005920:Glomerular Mesangium / Q000187:drug effects; D009569:Nitric Oxide / Q000378:metabolism*; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*; D010982:Platelet-Derived Growth Factor / Q000037:antagonists & inhibitors*; D051381:Rats; D062367:ortho-Aminobenzoates / Q000494:pharmacology*
D016207:Cytokines; D010975:Platelet Aggregation Inhibitors; D010982:Platelet-Derived Growth Factor; D062367:ortho-Aminobenzoates; D009569:Nitric Oxide; C012293:tranilast