title: Expression of interleukin-1 beta, interleukin-6 and tumour necrosis factor alpha by macrophages in canine lymph nodes with mineral-associated lymphadenopathy, granulomatous lymphadenitis or reactive hyperplasia.
abstract: The distribution of CD3+ T lymphocytes and the expression of interleukin-1 beta (IL-1 beta), interleukin-6 (IL-6) and tumour necrosis factor alpha (TNF alpha) by macrophages in canine lymph nodes with mineral-associated lymph-adenopathy, granulomatous lymphadenitis or reactive hyperplasia was examined immunohistochemically. In areas of preserved lymph node architecture, CD3+ T lymphocytes were located in the paracortex, with scattered cells in the follicular mantle and germinal centre, and small aggregates in the medullary cords. T cells were prominent within areas of diffuse granulomatous inflammation and formed a peripheral rim around microgranulomas. TNF alpha expression was demonstrated in macrophages from seven of 11 dogs with mineral-associated lymphadenopathy, one of five dogs with granulomatous lymphadenitis and three of five with reactive hyperplasia. Positive macrophage staining for IL-1 beta and IL-6 was seen in two lymph nodes with reactive hyperplasia. Lymph nodes from control (clinically normal) dogs showed no evidence of cytokine expression. Neutrophils expressing IL-1 beta were observed within the medullary sinuses of two lymph nodes with mineral-associated lymphadenopathy and one with reactive hyperplasia, and in the inflammatory infiltrate in one lymph node with granulomatous lymph-adenitis. The sensitivity of immunohistochemical examination as a means of detecting cytokines in abnormal tissue is discussed.
authors.affiliation: Department of Pathology, University of Bristol, Langford, UK.
authors.forename: M J
authors.initials: MJ
authors.lastname: Day
mesh_terms: D000818:Animals; D004283:Dog Diseases / Q000139:chemically induced / Q000473:pathology*; D004285:Dogs; D005260:Female; D007375:Interleukin-1 / Q000096:biosynthesis*; D015850:Interleukin-6 / Q000096:biosynthesis*; D008198:Lymph Nodes / Q000187:drug effects / Q000276:immunology / Q000473:pathology*; D008199:Lymphadenitis / Q000139:chemically induced / Q000473:pathology* / Q000662:veterinary*; D008206:Lymphatic Diseases / Q000139:chemically induced / Q000473:pathology* / Q000662:veterinary*; D008264:Macrophages / Q000378:metabolism*; D008297:Male; D008903:Minerals / Q000009:adverse effects*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*
chemical_list: D007375:Interleukin-1; D015850:Interleukin-6; D008903:Minerals; D014409:Tumor Necrosis Factor-alpha