Bombardier beetle based biosensor.
A biosensor constructed by combining an oxygen electrode with the liquid ejected by bombardier beetles (Pheropsophus aequinoctialis) immobolized on a collagen membrane is developed for hydrogen peroxide. The sensor presents a linear range of 2.0 x 10(-4)-2.0 x 10(-3) M and a reproductibility of approximately 2%. It shows a better lifetime than similar ones employing purified catalase. The selectivity is good since other enzymes are not present in the natural source employed.
Nakatani
H S
HS
Instituto de Química, Universidade Estadual de Campinas, Brazil.
de Oliveira Neto
G
G
Godinho
O E
OE
Aleixo
L M
LM
Zinner
K
K
D000818:Animals; D015374:Biosensing Techniques; D002138:Calibration; D002374:Catalase; D001517:Coleoptera / Q000201:enzymology*; D006861:Hydrogen Peroxide / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D016014:Linear Models; D008567:Membranes, Artificial; D009195:Peroxidase; D015203:Reproducibility of Results; D013696:Temperature
D008567:Membranes, Artificial; D006861:Hydrogen Peroxide; D002374:Catalase; D009195:Peroxidase