An experimental study of the mechanism of andrographis paniculata nees (APN) in alleviating the Ca(2+)-overloading in the process of myocardial ischemic reperfusion.
The aim of this experiment is to study the mechanism of APN in alleviating the Ca(2+)-overloading in dog model during the process of ischemic reperfusion. In comparison with the sustained ischemic group, the parameters in the ischemic reperfusion group demonstrated: Ca(2+) of ischemic region of myocardial cell increased (P < 0.05), Na+ increased remarkably (P < 0.01), the activity of Ca(2+)-ATPase dropped remarkably (P < 0.01), and MDA increased significantly (P < 0.01). Whereas in the group pretreated with APN, the Ca(2+) in the relevant area reduced (P < 0.05), Na+ decreased significantly (P < 0.01), the activity of Ca(2+)-ATPase and Na+-K+ ATPase increased remarkably (P < 0.01), and MDA decreased significantly (P < 0.01). These findings indicate tha APN may improve the activity of sarcolemma ATPase in alleviating the Ca(2+) and Na+ -overloading by decreasing the harmful effect of oxygen free radicals.
Guo
Z L
ZL
Department of Medicine, Tongji Hospital, Tongji Medical University, Wuhan.
Zhao
H Y
HY
Zheng
X H
XH
D000818:Animals; D002121:Calcium Channel Blockers / Q000494:pharmacology*; D000252:Calcium-Transporting ATPases / Q000378:metabolism; D004285:Dogs; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D015428:Myocardial Reperfusion Injury / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism
D002121:Calcium Channel Blockers; D004365:Drugs, Chinese Herbal; D008315:Malondialdehyde; D000252:Calcium-Transporting ATPases; D000254:Sodium-Potassium-Exchanging ATPase