Cutting apart V(D)J recombination.
The past year has seen major advances in our understanding of the recombination mechanism by which antibody and T cell receptor genes are assembled during lymphoid development. The initial cleavage events can be carried out in vitro by purified RAG1 and RAG/ protein. In addition, a number of genes involved in later steps of the reaction have been cloned, opening the way for an in-depth biochemical analysis of this critical developmental process.
Oettinger
M A
MA
Department of Molecular Biology, Massachusetts General Hospital, Boston 02114, USA.
D000818:Animals; D004254:DNA Nucleotidyltransferases / Q000378:metabolism; D004268:DNA-Binding Proteins; D015322:Gene Rearrangement, B-Lymphocyte / Q000235:genetics*; D015329:Gene Rearrangement, T-Lymphocyte / Q000235:genetics*; D018398:Homeodomain Proteins; D008957:Models, Genetic; D011506:Proteins / Q000502:physiology; D011995:Recombination, Genetic / Q000235:genetics*; D045567:VDJ Recombinases
D004268:DNA-Binding Proteins; D018398:Homeodomain Proteins; D011506:Proteins; C064655:V(D)J recombination activating protein 2; C064658:RAG-1 protein; D004254:DNA Nucleotidyltransferases; D045567:VDJ Recombinases