title: Exposure of postnatal rats to glucocorticoids suppresses the development of choline acetyltransferase-immunoreactive neurons: role of adrenal steroids in the development of forebrain cholinergic neurons.
abstract: Rat forebrain cholinergic neurons undergo dynamic developmental changes, showing a continuous increase in choline acetyl-transferase (ChAT) activity, during the early postnatal period. In adult rats, increases in circulating glucocorticoids result in decreases in activity of forebrain neuronal ChAT, thus raising the possibility that postnatal development of forebrain cholinergic neurons results from low levels of these hormones. In the rat, the first 2 weeks postnatally are characterized by very low levels of adrenal steroids. To understand the role of endogenous glucocorticoids in the development of forebrain cholinergic neurons, we studied the changes in ChAT immunoreactivity in forebrain cholinergic neurons of postnatal rats which had received daily subcutaneous injection of the synthetic glucocorticoid dexamethasone for 8 days. Immunohistochemical analysis of the rat pup forebrain revealed nearly complete obliteration of ChAT-immunoreactive neurons in the caudate-putamen, especially in the dorsolateral region of the rostral part. At the same stage, treatment with dexamethasone induced significant decreases in both number and length of dendritic branches of ChAT-immunoreactive neurons in the substantia innominata and the diagonal band. Despite the marked alterations in the caudate-putamen and diagonal band, the ChAT-immunoreactive neurons in other forebrain structures such as globus pallidus and medial septal nucleus showed little change. In the caudate-putamen, Nissl staining and specific labeling for nuclear DNA fragmentation exhibited no increase in number of dying cells following dexamethasone treatment, therefore indicating that the loss of ChAT immunoreactivity is not due to glucocorticoid-induced cholinergic cell death. These observations demonstrated that the development of cholinergic neurons in rat pups was inhibited by prolonged glucocorticoid exposure, suggesting that low levels of adrenal steroids may promote the postnatal development of these neurons.
authors.affiliation: Department of Anatomy and Neurobiology, Kyoto Prefectural University of Medicine, Japan.
authors.forename: Z
authors.initials: Z
authors.lastname: Hu
authors.forename: K
authors.initials: K
authors.lastname: Yuri
authors.forename: T
authors.initials: T
authors.lastname: Ichikawa
authors.forename: M
authors.initials: M
authors.lastname: Kawata
mesh_terms: D000305:Adrenal Cortex Hormones / Q000502:physiology*; D000818:Animals; D000831:Animals, Newborn / Q000254:growth & development; D017209:Apoptosis / Q000187:drug effects; D002795:Choline O-Acetyltransferase / Q000187:drug effects / Q000378:metabolism*; D002799:Cholinergic Fibers / Q000187:drug effects / Q000502:physiology*; D004253:DNA Nucleotidylexotransferase / Q000378:metabolism; D004277:DNA, Single-Stranded / Q000378:metabolism; D003907:Dexamethasone / Q000494:pharmacology; D005260:Female; D005938:Glucocorticoids / Q000494:pharmacology; D007150:Immunohistochemistry; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D011247:Pregnancy; D016548:Prosencephalon / Q000033:anatomy & histology / Q000187:drug effects / Q000254:growth & development* / Q000276:immunology; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000305:Adrenal Cortex Hormones; D004277:DNA, Single-Stranded; D005938:Glucocorticoids; D003907:Dexamethasone; D002795:Choline O-Acetyltransferase; D004253:DNA Nucleotidylexotransferase