Use of a single slide trichrome-stained concentrate for the detection of intestinal parasites. Stained concentration procedure for ova and parasites.
The detection and identification of intestinal parasites was studied using a single slide of a trichrome-stained concentrate made from a cupric-polyvinyl alcohol (PVA)-preserved specimen. When saline was used in place of formalin in the concentration procedure, it was possible to adequately stain the concentrate with a modified-trichrome stain. This was compared to a more traditional two-slide ova and parasite (O&P) procedure. Of the 1,000 specimens evaluated, a total of 261 parasites were detected using the single slide, modified trichrome-stained concentrate compared to 249 from the formalin/Hemo-De wet mount and 226 from the unconcentrated trichrome stain. The detection rate of the single slide method was comparable to the combined detection rate of the two-slide method. The saline/Hemo-De concentration procedure has the advantage of using nontoxic saline in place of formaldehyde. This new method is more cost-effective because it requires the processing and evaluation of only one slide.
Hale
D C
DC
Department of Pathology, University of Utah Medical School, Salt Lake City, USA.
Carroll
K
K
Kucera
J R
JR
Aldeen
W E
WE
D000818:Animals; D002498:Centrifugation; D056890:Eukaryota / Q000469:parasitology*; D005069:Evaluation Studies as Topic; D005243:Feces / Q000469:parasitology; D006376:Helminths / Q000469:parasitology*; D006801:Humans; D007411:Intestinal Diseases, Parasitic / Q000175:diagnosis* / Q000469:parasitology; D010063:Ovum / Q000166:cytology; D013048:Specimen Handling / Q000379:methods*; D013194:Staining and Labeling / Q000379:methods*