Evaluation of the fully automated Cobas Core enzyme immunoassay for the quantitation of antibodies against hepatitis B virus surface antigen.
The Roche Cobas Core automated enzyme immunoassay of antibodies against hepatitis B virus surface antigen (anti-HBs) was evaluated using a protocol complying with the Société Française de Biologie Clinique guidelines. Results showed good precision (CV below 6 and 9% for intra- and inter-assay precision) and accuracy (according to the Paul-Ehrlich-Institute standard); the detection limit of the test was 2 IU/l. The manufacturer's specifications were confirmed and a satisfactory correlation (r = 0.901) was found with an automated method (IMx, Abbott) used for comparison.
Doche
C
C
Laboratoire d'Immunologie et de Biologie de l'Inflammation, Centre Hospitalier Lyon-Sud, Pierre-Bénite, France.
Thomé
M
M
Dimet
I
I
Bienvenu
J
J
D005069:Evaluation Studies as Topic; D006509:Hepatitis B / Q000175:diagnosis / Q000517:prevention & control*; D006510:Hepatitis B Antibodies / Q000097:blood*; D006514:Hepatitis B Surface Antigens / Q000032:analysis / Q000276:immunology*; D006515:Hepatitis B virus / Q000276:immunology; D006801:Humans; D007124:Immunoenzyme Techniques / Q000592:standards; D015203:Reproducibility of Results
D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens