Structural and immunochemical studies on the lipopolysaccharide of the 'T-antigen'-containing mutant Proteus mirabilis R14/1959.
In DOC-PAGE, lipopolysaccharide (LPS) of Proteus mirabilis R14/1959 (Rb-type) mutant showed a ladder-like migration pattern indicating the presence of a high molecular weight polysaccharide chain. The isolated polysaccharide, called T-antigen because of similarity with the T1 chain of Salmonella friedenau LPS, contained D-glucose, D-galacturonic acid (D-GalA), and D-GlcNAc in molar ratios 2:1:1 and was structurally different from the O-antigen of the parental S-strain P. mirabilis S1959 but identical to the O-antigen of another S-strain Proteus penneri 42. The importance of a D-GalA(L-Lys)-containing epitope, most likely present in the core region of LPS, and of GalA present in the T-antigen chain in manifesting the serological specificity of P. mirabilis R14/1959 were revealed using rabbit polyclonal homologous and heterologous R- and O-specific antisera and the appropriate antigens, including synthetic antigens which represent partial structures of various Proteus LPS.
Bartodziejska
B
B
Institute of Microbiology and Immunology, University of Lodz, Poland.
Radziejewska-Lebrecht
J
J
Lipinska
M
M
Knirel
Y A
YA
Kononov
L O
LO
Chernyak
A Y
AY
Mayer
H
H
Rozalski
A
A
D000818:Animals; D000942:Antigens, Bacterial / Q000737:chemistry* / Q000276:immunology*; D015153:Blotting, Western; D002240:Carbohydrate Sequence; D003429:Cross Reactions; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000276:immunology*; D008969:Molecular Sequence Data; D009154:Mutation / Q000276:immunology*; D019081:O Antigens / Q000737:chemistry / Q000276:immunology; D011513:Proteus mirabilis / Q000235:genetics* / Q000276:immunology*; D011817:Rabbits
D000942:Antigens, Bacterial; D008070:Lipopolysaccharides; D019081:O Antigens