title: Retrotransposon BARE-1 is a major, dispersed component of the barley (Hordeum vulgare L.) genome.
abstract: The barley BARE-1 is a transcribed, copia-like retroelement with well-conserved functional domains, an active promoter, and a copy number of at least 3 x 10(4). We examined its chromosomal localization by in situ hybridization. The long terminal repeat (LTR) probe displayed a uniform hybridization pattern over the whole of all chromosomes, excepting paracentromeric regions, telomeres, and nucleolar organizer (NOR) regions. The integrase probe showed a similar pattern. The 5'-untranslated leader (UTL) probe, expected to be the most rapidly evolving component, labeled chromosomes in a dispersed and non-uniform manner, concentrated in the distal regions, possibly indicating a targe site preference.
authors.affiliation: Institute of Biotechnology, University of Helsinki, Finland.
authors.forename: A
authors.initials: A
authors.lastname: Suoniemi
authors.forename: K
authors.initials: K
authors.lastname: Anamthawat-Jónsson
authors.forename: T
authors.initials: T
authors.lastname: Arna
authors.forename: A H
authors.initials: AH
authors.lastname: Schulman
mesh_terms: D001483:Base Sequence; D002874:Chromosome Mapping; D017931:DNA Primers; D018745:Genome, Plant; D001467:Hordeum / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D012091:Repetitive Sequences, Nucleic Acid; D018626:Retroelements
chemical_list: D017931:DNA Primers; D018626:Retroelements