Heat shock elements are involved in heat shock promoter activation during tobacco seed maturation.
The soybean Gmhsp 17.3-B heat shock promoter is developmentally regulated in transgenic tobacco, as indicated by the constitutive expression of a beta-glucuronidase reporter in seeds [16]. In this paper, we show that both the heat shock promoter-driven beta-glucuronidase activity and the mRNA of the endogenous Nthsp18P gene accumulate coincident with the onset of seed desiccation. Deletions of the soybean Gmhsp17.3-B promoter, encompassing the heat shock element (HSE)-containing regions, revealed a co-localization of sequences responsible for heat induction and developmental expression. Moreover, synthetic HSEs fused to a TATA box sequence had the potential to stimulate the developmental expression of a GUS reporter gene in seeds of transgenic plants.
Prändl
R
R
Universität Tübingen, Biologisches Institut, Lehrstuhl für Allgemeine Genetik, Germany.
Schöffl
F
F
D001483:Base Sequence; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D017930:Genes, Reporter; D005966:Glucuronidase / Q000235:genetics; D006360:Heat-Shock Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D030821:Plants, Genetically Modified; D010947:Plants, Toxic; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics; D012639:Seeds / Q000235:genetics*; D017384:Sequence Deletion; D016385:TATA Box; D014026:Nicotiana / Q000196:embryology / Q000235:genetics*
D006360:Heat-Shock Proteins; D012333:RNA, Messenger; D005966:Glucuronidase