title: Full-lenth cloning, expression and cellular localization of rat plasmolipin mRNA, a proteolipid of PNS and CNS.
abstract: We have isolated a 1.476 bp cDNA (NTII11) representing a transcript that is differntially expressed during sciatic nerve development and regeneration in the rat. Nucleotide sequence comparison indicates partial identity with a recently isolated plasmolipin cDNA. However, our clone extends the published sequence by 234 bp at the 5' end and predicts a protein that contains an additional 25 amino acids at th N-terminus. The open reading frame of th NTII11 transcript encodes a 19.4 kDa protein with four putative transmembrane domains. Northern blot analyses revealed a tissue-specific expression was confirmed by in situ hybridization, and cellular localization of plasmolipin mRNA was demonstrated in Schwann cells of the sciatic nerve and in glial cells of myelinated brain structures. The steady-state levels of plasmolipin mRNA were markedly altered (i) during development of sciatic nerve and brain. (ii) after sciatic nerve injury, and (ii) in cured Schwann cells maintained under different conditions of cell growth and arrest. Our data indicate a function of plasmolipin during myelination in the central as well as in the peripheral nervous system.
authors.affiliation: Department of Neurology, University of Düsseldorf, Moorenstrasse 5,D-40558 Düsseldorf, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Gillen
authors.forename: M
authors.initials: M
authors.lastname: Gleichmann
authors.forename: R
authors.initials: R
authors.lastname: Greiner-Petter
authors.forename: G
authors.initials: G
authors.lastname: Zoidl
authors.forename: S
authors.initials: S
authors.lastname: Kupfer
authors.forename: F
authors.initials: F
authors.lastname: Bosse
authors.forename: J
authors.initials: J
authors.lastname: Auer
authors.forename: H W
authors.initials: HW
authors.lastname: Müller
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001923:Brain Chemistry; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D015723:Gene Library; D017403:In Situ Hybridization; D008565:Membrane Proteins; D008958:Models, Molecular; D009186:Myelin Sheath / Q000378:metabolism; D062806:Myelin and Lymphocyte-Associated Proteolipid Proteins; D009409:Nerve Crush; D009416:Nerve Regeneration; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D009457:Neuroglia / Q000378:metabolism; D011487:Protein Conformation; D011510:Proteolipids / Q000032:analysis / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics*; D051381:Rats; D017208:Rats, Wistar; D012583:Schwann Cells / Q000378:metabolism; D012584:Sciatic Nerve / Q000737:chemistry* / Q000293:injuries / Q000502:physiology; D012689:Sequence Homology, Nucleic Acid
chemical_list: D018076:DNA, Complementary; D008565:Membrane Proteins; D062806:Myelin and Lymphocyte-Associated Proteolipid Proteins; D009419:Nerve Tissue Proteins; C489076:Pllp protein, rat; D011510:Proteolipids; D012333:RNA, Messenger