[In situ hybridization of cells infected by Chlamydia trachomatis].
Hybridization in situ of chlamydia trachomatis allows identification of the bacteria. Moreover exact chlamydia trachomatis localization at tissue level can be demonstrated. It seems to us very important to know for instance in conjunctiva were chlamydia trachomatis are located. We used DNA DIGOXIGENIN labelled plasmidic probe of 503 b.p. Detection of chlamydia trachomatis on paraffin embedded cells becomes possible. Specificity is 100% as plasmidic sequence is not found in other bacteria. Sensitivity seems to be good quality on experimental model. At present time, it is under clinical evaluation. This method could complete PCR for chlamydia detection in case of controversial diagnosis.
Mortemousque
B
B
Centre Jean Abadie, Hôpital Pollegrin, Université de Bordeaux II.
Verin
P
P
Bebear
C
C
de Barbeyrac
B
B
Gendre
P
P
D002690:Chlamydia Infections / Q000382:microbiology*; D002692:Chlamydia trachomatis / Q000235:genetics*; D015342:DNA Probes; D004269:DNA, Bacterial / Q000032:analysis*; D004076:Digoxigenin; D006801:Humans; D017403:In Situ Hybridization
D015342:DNA Probes; D004269:DNA, Bacterial; D004076:Digoxigenin
Hybridation in situ sur cellules infectées par chlamydia trachomatis.