Molecular analysis of the gene cluster involved in cephalosporin biosynthesis from Lysobacter lactamgenus YK90.
Determination of the nucleotide sequence downstream from the Lysobacter lactamgenus pcbC gene encoding isopenicillin N synthase revealed that five open-reading frames (ORF) including the pcbC gene were tightly linked in the same orientation. Each ORF has the remarkable feature of the protein-coding frame in the DNA sequence with a high G+C content. Expression in Escherichia coli and a comparison of the deduced amino acid sequences with published sequences showed that the gene cluster contained a deacetoxycephalosporin C synthetase (DAOCS) gene (cefE), an ORF having homology with the Cephalosporium acremonium DAOCS/deacetylcephalosporin C synthetase gene (cefEF), an isopenicillin N epimerase gene(cefD), and a beta-lactamase gene. The gene order was pcbC-cefE-ORF3-cefD-beta-lactamase.
Kimura
H
H
Fermentation Center, Takeda Chemical Industries Ltd., Yodogawa-ku, Osaka, Japan.
Izawa
M
M
Sumino
Y
Y
D000595:Amino Acid Sequence; D001483:Base Sequence; D002511:Cephalosporins / Q000096:biosynthesis*; D005798:Genes, Bacterial; D006090:Gram-Negative Bacteria / Q000235:genetics*; D008969:Molecular Sequence Data; D005810:Multigene Family; D016366:Open Reading Frames; D010088:Oxidoreductases / Q000235:genetics*
D002511:Cephalosporins; D010088:Oxidoreductases; C038370:isopenicillin N synthetase