Capillary high-performance liquid chromatography-fast atom bombardment mass spectrometry of 24 cephem antibiotics.
Using capillary high-performance liquid chromatography (HPLC)-fast atom bombardment (FAB) mass spectrometry (MS), both positive and negative FAB mass spectra of 24 cephem antibiotics with diethanolamine (DEA) and glycerol (GLY) as matrices are presented. In the positive mode, an internal quasi-molecular peak together with relatively abundant fragment peaks were obtained from all 24 drugs with both matrices, though DEA provided more information on molecular mass of a compound than did GLY for some drugs. In the negative mode, the background was generally lower than that in the positive, but neither the quasi-molecular nor molecular peak was detected in several drugs with either matrix. The drugs were isolated from serum samples using an octadecyl reversed-phase cartridge; recoveries were generally over 60%, With this isolation and the capillary HPLC-FAB-MS in the positive mode, ceftriaxone and cefazolin, two of the most popular cephem antibiotics, were successfully identified in 0.5 ml of sera obtained from a clinical or an autopsy case.
Kobayashi
K
K
Department of Legal Medicine, Nagoya University School of Medicine, Japan.
Sato
K
K
Mizuno
Y
Y
Katsumata
Y
Y
D000328:Adult; D000368:Aged; D001344:Autopsy; D002437:Cefazolin / Q000097:blood* / Q000737:chemistry; D002443:Ceftriaxone / Q000097:blood* / Q000737:chemistry; D002511:Cephalosporins / Q000097:blood* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004983:Ethanolamines / Q000737:chemistry; D005260:Female; D005990:Glycerol / Q000737:chemistry; D006801:Humans; D008297:Male; D016339:Spectrometry, Mass, Fast Atom Bombardment / Q000379:methods*
D002511:Cephalosporins; D004983:Ethanolamines; D002443:Ceftriaxone; C020283:diethanolamine; D002437:Cefazolin; D005990:Glycerol