Synthesis of leader RNA and editing of P mRNA during transcription by rinderpest virus.
Purified rinderpest virus was earlier shown to transcribe in vitro, all virus-specific mRNAs with the promoter-proximal N mRNA being the most abundant. Presently, this transcription system has been shown to synthesize full length monocistronic mRNAs comparable to those made in infected cells. Small quantities of bi- and tricistronic mRNAs are also synthesized. Rinderpest virus synthesizes in vitro, a leader RNA of approximately 55 nucleotides in length. Purified rinderpest virus also exhibits RNA editing activity during the synthesis of P mRNA as shown by primer extension analysis of the mRNA products.
Ghosh
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A
Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore, India.
Nayak
R
R
Shaila
M S
MS
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002522:Chlorocebus aethiops; D004279:DNA, Viral; D008969:Molecular Sequence Data; D010750:Phosphoproteins / Q000235:genetics*; D017393:RNA Editing; D012333:RNA, Messenger / Q000096:biosynthesis*; D012367:RNA, Viral; D012302:Rinderpest virus / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic; D014709:Vero Cells; D014764:Viral Proteins / Q000235:genetics*
D004279:DNA, Viral; D010750:Phosphoproteins; D012333:RNA, Messenger; D012367:RNA, Viral; D014764:Viral Proteins; C076637:P gene protein, Rinderpest virus