[Monoclonal antibodies to 8-oxo-2'-deoxyguanosine (8-hydroxyguanosine). Characteristics and use for determining DNA damage by active forms of oxygen].
It has presently been established that the guanine base is one of the most sensitive and biologically significant target for the damaging action of active oxygen species on DNA, 7.8-dihydroxy-8-oxoguanine (8-oxoguanine, 8-hydroxyguanine) being the major degradation product and the most essential biomarker for DNA damage by active oxygen species. Murine monoclonal antibodies (MAbs) specifically recognizing 8-oxoguanine have been raised. Using competitive solid phase immunoenzymatic assay. (IEA) with peroxidase-antiperoxidase (PAP-method), a quantitative assay of this degradation product and characterization of affinity and specificity of MAbs have been carried out. The affinity constant (Kaff) Mabs for 8-oxo-2'-deoxyguanosine is equal to 1.3-10(6), that for 8-oxo-guanosine-to 1.10(6), exceeding by more than three orders of magnitude the Ka values for natural guanyl nucleosides and other possible cross-structure analogs. IEA was used to determine the DNA degradation product in gamma-irradiated DNA. The radiation-chemical yield of 8-oxoguanine (G = 0.57 molecules per 100 eV) is consistent with those obtained by other methods.
Bruskov
V I
VI
bruskov@venus.iteb.serpukhov.su
Gaziev
A I
AI
Malakhova
L V
LV
Mantsygin
Iu A
IuA
Morenkov
O S
OS
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000302:isolation & purification; D000915:Antibody Affinity; D002417:Cattle; D004247:DNA / Q000528:radiation effects; D004249:DNA Damage; D006151:Guanosine / Q000031:analogs & derivatives* / Q000276:immunology; D007124:Immunoenzyme Techniques; D017382:Reactive Oxygen Species
D000911:Antibodies, Monoclonal; D017382:Reactive Oxygen Species; D006151:Guanosine; C046215:8-hydroxyguanosine; D004247:DNA
Monoklonal'nye antitela k 8-okso-2'-dezoksiguanozinu (8-gidroksiguanozinu). Kharakteristika i ispol'zovanie dlia opredeleniia povrezhdeniia DNK aktivnymi formami kisloroda.