Morphological and hemostatic changes in rats with abdominal arterial prosthesis.
We evaluated the changes over time in hemostatic factors during ongoing arterial thrombosis in rats, as induced by a loop-shaped aortic prosthesis. Moreover, we investigated this condition by inspecting in parallel local thrombus growth, systemic vascular prostacyclin and t-PA production. One minute after loop insertion, activated platelets spread on the internal surface of the prosthesis and 24 hrs later numerous platelet aggregates supported by a fibrin network could be observed. However, no evidence for platelet activation could be concomitantly found in peripheral blood. A sustained increased in PGI2 formation was detected together with a progressive increase in plasma fibrinolytic activity during thrombus growth. The levels of fibrinogen as well as antithrombin III (ATIII) and heparin cofactor II (HCII) activities were steadily increased in loop-bearing animals. In conclusion, the dynamic phases of thrombus formation, in an aortic prosthesis, produce changes in vascular function and in hemostatic factors at the level of systemic blood.
Chabielska
E
E
Laboratory of Thrombosis Pharmacology, Istituto di Ricerche Farmacologiche Mario Negri, Consorzio Mario Negri Sud, Santa Maria Imbaro, Italy.
Kolpakov
V
V
D'Adamo
M C
MC
De Curtis
A
A
Buczko
W
W
Iacoviello
L
L
Donati
M B
MB
D015121:6-Ketoprostaglandin F1 alpha / Q000097:blood; D000818:Animals; D001011:Aorta; D001779:Blood Coagulation Factors / Q000378:metabolism; D001807:Blood Vessel Prosthesis; D005337:Fibrin / Q000378:metabolism; D005342:Fibrinolysis; D006487:Hemostasis; D007958:Leukocyte Count; D008297:Male; D010974:Platelet Aggregation; D010976:Platelet Count; D019736:Prostheses and Implants; D051381:Rats; D013927:Thrombosis / Q000473:pathology*; D013997:Time Factors
D001779:Blood Coagulation Factors; D015121:6-Ketoprostaglandin F1 alpha; D005337:Fibrin