Cellular differentiation and development of pyloric mucosal metaplasia in the human gall-bladder.
The development of pyloric mucosal metaplasia (PMM) with regard to cellular differentiation in the human gallbladder was studied by mucin staining (paradoxical concanavalin A (Con A), galactose oxidase-Schiff (GOS) and alcian blue (pH 2.5) PAS (AB-PAS) and immunohistochemistry (pepsinogen II (PgII) and SH-9, and proliferating cell nuclear antigen (PCNA). PMM was divided into three stages of development by three-dimensional (3D) computer graphic reconstruction analysis. In the early stage, a transitional zone of PCNA positive cells was observed between areas of SH-9 and/or GOS reactive cells and class III and/or Pg II positive cells in flat monolayered epithelium. In the middle stage, shallow pits became apparent as areas enlarged, with these becoming deeper in the advanced stage, whereby SH-9 and/or GOS reactive cells and class III and/or Pg II positive cells were observed at the upper and lower portion of the pits, respectively, with PCNA-positive cells forming a narrow zone between the two cell populations. Consequently, the structure of PMM gradually resembles that of the normal gastric pyloric mucosa.
Shimizu
M
M
Laboratory of Pathology, Aichi Cancer Center Research Institute, Nagoya, Japan.
Kitoh
K
K
Fujimitsu
Y
Y
Inada
K
K
Ichinose
M
M
Miki
K
K
Yamao
K
K
Nakazawa
S
S
Shamoto
M
M
Tatematsu
M
M
D002454:Cell Differentiation / Q000502:physiology; D005260:Female; D005704:Gallbladder / Q000276:immunology / Q000473:pathology* / Q000601:surgery; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry; D008297:Male; D008679:Metaplasia / Q000276:immunology / Q000473:pathology*; D008875:Middle Aged; D008954:Models, Biological; D009092:Mucous Membrane / Q000276:immunology / Q000473:pathology; D011708:Pylorus / Q000276:immunology / Q000473:pathology* / Q000601:surgery; D013997:Time Factors