[Competitive interaction of the fluorescent probe 4-(n-dimethylaminostyryl)-1-methylpyridinium n-toluolsulfonate and lysozyme with lysosomes].
Competitive binding of fluorescent probe 4-(n-dimethylaminostyryl)-1-methylpyiridinium n-toluenesulfonate and lysozyme to liposomes composed of phosphatidylcholine and diphosphatidylglycerol has been investigated. Association constants and binding stoichiometry for probe and protein have been estimated. Increase of the bilayer negative charge was found to be followed by the increase of binding constant and number of phospholipids interacting with lysozyme.
Gorbenko
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GP
D001667:Binding, Competitive; D005456:Fluorescent Dyes / Q000378:metabolism*; D008051:Lipid Bilayers; D008081:Liposomes; D009113:Muramidase / Q000378:metabolism*; D011726:Pyridinium Compounds / Q000378:metabolism*
C032315:4-(4-dimethylaminostyryl)-1-methylpyridinium; D005456:Fluorescent Dyes; D008051:Lipid Bilayers; D008081:Liposomes; D011726:Pyridinium Compounds; D009113:Muramidase
Konkurentnoe vzaimode istvie fluorestsentnogo zonda 4-(n-dimetilaminostiril)-1-metilpiridiniī n-toluolsul'fonata i lizotsima s lisosomami.