Effect of 8-sulfophenyl theophylline on endogenous noradrenaline release from sympathetic nerves of the rabbit ear artery.
1. The release of endogenous noradrenaline (NA) and adenyl purine (ATP, ADP, AMP and adenosine) from the rabbit ear artery, evoked by electrical stimulation (ES; 16 Hz), was examined. 2. ES evoked a significant release of NA and purine; the ratio of the amount of total purine released to NA released was approximately 180 on a molar base. 3. ES-evoked purine release was significantly reduced by the denudation of the endothelium and abolished by the alpha 1-adrenoceptor antagonist, prazosin (1 mumol/L). 4. ES-evoked NA release was significantly reduced by a P1-purinoceptor antagonist, 8-sulfophenyl theophylline (8SPT). Purine release was slightly reduced by 8SPT. 5. These results suggest that endogenous NA released by ES results in the release of a large amount of purine, which may, in turn, increase the release of NA by acting on prejunctional purinoceptors on sympathetic nerve terminals.
Ishii
R
R
Department of Pharmacology, Meiji College of Pharmacy, Tokyo, Japan.
Shinozuka
K
K
Kunitomo
M
M
Hashimoto
T
T
Takeuchi
K
K
D000818:Animals; D001158:Arteries / Q000294:innervation*; D004423:Ear / Q000098:blood supply*; D004558:Electric Stimulation / Q000379:methods; D009638:Norepinephrine / Q000037:antagonists & inhibitors / Q000378:metabolism*; D010525:Peripheral Nerves / Q000187:drug effects / Q000378:metabolism*; D011224:Prazosin / Q000494:pharmacology; D058915:Purinergic P1 Receptor Antagonists; D011817:Rabbits; D013564:Sympathetic Nervous System / Q000187:drug effects / Q000378:metabolism*; D013806:Theophylline / Q000031:analogs & derivatives* / Q000494:pharmacology
D058915:Purinergic P1 Receptor Antagonists; C031433:8-(4-sulfophenyl)theophylline; D013806:Theophylline; D009638:Norepinephrine; D011224:Prazosin