Liquid chromatographic assay for a butenolide endothelin antagonist (PD 156707) in plasma.
A sensitive and selective liquid chromatographic assay for determining the non-peptide endothelin A receptor antagonist PD 156707 (I) in rat plasma has been developed and validated. The analyte was isolated from matrix by solid-phase extraction. Liquid chromatographic separation was achieved isocratically on a 3.2 mm I.D., ODS column with a mobile phase of acetonitrile-ammonium phosphate (50 mM, pH 3.5) (44:56, v/v). Column effluent was monitored fluorometrically. Peak-height ratios (analyte/IS) were proportional to I concentrations in rat plasma from 25 to 1000 ng/ml. Assay precision and accuracy for I, based on quality controls, was 9.5% relative standard deviation, with relative error of +/- 6.5%. The quantitation limit was 25 ng/ml for a 200-microliters sample aliquot.
Rossi
D T
DT
Department of Pharmacokinetics and Drug Metabolism, Parke-Davis Pharmaceutical Research, Division of Warner Lambert Company, Ann Arbor, MI 48105, USA.
Hallak
H
H
Bradford
L
L
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004149:Dioxoles / Q000097:blood*; D065128:Endothelin Receptor Antagonists; D051381:Rats; D044022:Receptor, Endothelin A; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence
D004149:Dioxoles; D065128:Endothelin Receptor Antagonists; C093321:PD 156707; D044022:Receptor, Endothelin A