Isolation, characterization and immunolocalization of a globin-like antigen from Ostertagia ostertagi adults.
Western blot analysis using an anti-globin rabbit serum Rb94 revealed a major band of 17 kDa in extracts of Ostertagia ostertagi adults and 4th-stage larvae. The adult stage globin-like antigen (OoAdGlb) was purified from total worm extracts by liquid chromatography. The protein has an estimated molecular mass of 36 kDa under non-reducing conditions, suggesting a dimeric structure containing 2 non-covalently linked 17 kDa monomers. Tryptic peptides were sequenced and showed strong similarities with the globins of free-living and parasitic nematodes. Immunolocalization revealed the presence of this globin-like antigen in the body wall musculature and/or the cuticle of O. ostertagi adults. An enzyme-linked immunosorbent assay based on the purified OoAdGlb showed no differences in response between calves infected by O. ostertagi and/or Cooperia oncophora and the negative controls.
de Graaf
D C
DC
Department of Parasitology, University of Gent, Merelbeke, Belgium.
Berghen
P
P
Moens
L
L
de Marez
T M
TM
Raes
S
S
Blaxter
M L
ML
Vercruysse
J
J
D000595:Amino Acid Sequence; D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis; D000947:Antigens, Helminth / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D015153:Blotting, Western; D002417:Cattle; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004797:Enzyme-Linked Immunosorbent Assay; D019084:Fluorescent Antibody Technique, Indirect; D005914:Globins / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D007106:Immune Sera / Q000276:immunology; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009559:Nippostrongylus / Q000276:immunology; D010028:Ostertagia / Q000276:immunology*; D011817:Rabbits; D016415:Sequence Alignment; D014254:Trichostrongylus / Q000276:immunology
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D007106:Immune Sera; D005914:Globins