Revertant analysis of J-K mutations in the encephalomyocarditis virus internal ribosomal entry site detects an altered leader protein.
The internal ribosomal entry site (IRES) of picornaviruses consists of various sequence and structural elements that collectively impart translational function to the genome. By engineering substitution and deletion mutations into the J-K elements of the encephalomyocarditis virus IRES, translationally defective viruses with small-plaque phenotypes were generated. From these, 60 larger-plaque revertant viruses were isolated and characterized, and their sequences were compared with a structural model of the IRES. The data provide confirming evidence for the existence of helix J3 within stem J but suggest that helix J1 is 3 bp longer than previously estimated. They also suggest that previously modeled stems L and M should be replaced by an alternative structure. One reversion mutation was mapped to the leader protein coding region. This change of leader amino acid 20 from Pro to Ser increased the viral plaque size dramatically but did not alter the cell-free translational activity of the mutated, parental IRES.
Hoffman
M A
MA
Institute for Molecular Virology, University of Wisconsin, Madison 53706, USA.
Palmenberg
A C
AC
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D017931:DNA Primers; D003673:Defective Viruses / Q000235:genetics / Q000502:physiology; D004686:Encephalomyocarditis virus / Q000235:genetics* / Q000502:physiology; D008961:Models, Structural; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009690:Nucleic Acid Conformation; D010641:Phenotype; D017354:Point Mutation; D014176:Protein Biosynthesis; D021382:Protein Sorting Signals / Q000235:genetics / Q000378:metabolism*; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012156:Reticulocytes / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism / Q000821:virology*; D014158:Transcription, Genetic; D014162:Transfection; D010948:Viral Plaque Assay
D017931:DNA Primers; D021382:Protein Sorting Signals; D012367:RNA, Viral