Quantitative assessment of apatite formation via a biomimetic method using quartz crystal microbalance.
Quantitative assessment of hydroxyapatite formation on a gold surface via the biomimetic method, composed of a nucleation step in a simulated body fluid (SBF) containing glass powders and a subsequent apatite growth step in glass powder-free SBF, was made using a quartz crystal microbalance (QCM) technique. The frequency change of the QCM linearly increased with increasing soaking time, and largely depended on the nucleation period. The growth rates, defined as daily increase in thickness, increased monotonicly with an increasing nucleation period of up to 96 h, thereafter being constant at 2.0 microns/day. The growth rate of the apatite layer increased with increasing temperature of the SBF: 0.9, 2.0, and 3.8 microns/day at 25, 37, and 50 degrees C, respectively. The Arrhenius-type activation energy for the growth of apatite was 47.3 kJ/mol. The QCM method was found to be a very powerful tool for quantitative, in situ measurement of precipitation and growth of apatite in real time.
Tanahashi
M
M
Department of Bioengineering, National Cardiovascular Center Research Institute, Osaka, Japan.
Kokubo
T
T
Matsuda
T
T
D001031:Apatites; D001826:Body Fluids; D003460:Crystallization; D005898:Glass; D006046:Gold; D006801:Humans; D007700:Kinetics; D008855:Microscopy, Electron, Scanning; D008954:Models, Biological; D011791:Quartz; D013997:Time Factors
D001031:Apatites; D011791:Quartz; D006046:Gold