[Early diagnosis of HCMV infections in patients undergoing transplantation of the kidney].
HCMV infection is a major cause of morbidity and mortality following kidney transplantation. Clinical diagnosis is difficult, and rapid and sensitive diagnostic methods are needed since antiviral therapy is available. One hundred-forty-five consecutive kidney-transplanted patients were studied during a period of three months after transplantation. For laboratory diagnosis of HCMV infection, we looked for the presence of pp-65 antigen in polymorphonuclear leukocytes, HCMV-DNA and IgM. Demonstration of HCMV pp-65 antigen by immunofluorescence and HCMV DNA by PCR in leukocytes were efficient methods for early diagnosis of infection.
Cavallo
R
R
Istituto di Microbiologia, Università degli Studi di Torino.
Sinesi
F
F
Re
D
D
Piovesan
F
F
Fora
R
R
Lembo
D
D
Segoloni
G P
GP
Turello
E
E
Negro Ponzi
A
A
D000956:Antigens, Viral / Q000032:analysis*; D003586:Cytomegalovirus Infections / Q000175:diagnosis* / Q000276:immunology; D004279:DNA, Viral / Q000032:analysis; D006801:Humans; D016867:Immunocompromised Host; D007074:Immunoglobulin G / Q000032:analysis*; D007075:Immunoglobulin M / Q000032:analysis*; D016030:Kidney Transplantation
D000956:Antigens, Viral; D004279:DNA, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M
Diagnosi precoce di infezione da HCMV in pazienti sottoposti a trapianto di rene.