[The effect of hemolysate and calcium ions on transport ATPase activity in guinea pig erythrocytes].
Influence of 0.1, 0.3 and 1.0 microM Ca++ on Na,K-ATPases in different membrane preparations of guinea pig erythrocytes was studied both in presence and in absence of haemolysate. The incubation with the haemolysate and 0.1 or 0.3 microM Ca++ increased significantly the activity of the Na,K-ATPase in all membrane preparations used. The increasing Ca++ concentrations (up to 1 microM) led to the loss of the activating effect of the haemolysate in membrane preparations with lack of a part of the cytoskeleton proteins, and also decreased sharply its activity in all the preparations in absence of haemolysate. The latter also increased the Ca-ATPase activity irrespectively of the integrity of cytoskeleton structures (may be because of calmodulin action). The results obtained suggest that the cytoskeleton structures mediate the intracellular Na,K-ATPase activator action.
Matskevich
Iu A
IuA
Kazennov
A M
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D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002118:Calcium / Q000494:pharmacology*; D000252:Calcium-Transporting ATPases / Q000097:blood / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004591:Electrophoresis, Polyacrylamide Gel; D004910:Erythrocyte Membrane / Q000187:drug effects* / Q000201:enzymology; D004912:Erythrocytes / Q000187:drug effects / Q000201:enzymology; D006168:Guinea Pigs; D006461:Hemolysis; D008297:Male; D000254:Sodium-Potassium-Exchanging ATPase / Q000097:blood / Q000187:drug effects*
D000252:Calcium-Transporting ATPases; D000254:Sodium-Potassium-Exchanging ATPase; D002118:Calcium
Vliianie gemolizata i ionov kal'tsiia na aktivnost' transportnykh ATFaz éritrotsitov morskoĭ svinki.