[Expression of c-myc gene and biosynthesis of biological macromolecules in antisense transfectant HLR60-9].
The recombinant plasmid PGC was constructed for transcription unit of c-myc gene with bi-direction in vitro, to make RNA probes for detection of c-myc mRNA and antisense RNA expression of transfectant HLR60-9, which was obtained from HL60 cells transfected with inducible c-myc antisense RNA expression plasmid. The results from HLR60-9 cells induced by Cd2+ indicated that expression of c-myc antisense RNA increased with Cd2+ concentration and exposure time, while c-myc mRNA expression progressively reduced. Using immunohistochemical technique no c-myc P62 protein expression was detected. The incorporation of 3H-TdR,3H-UR,3H-Leu revealed significant suppression of DNA, RNA and protein biosyntheses. It is suggested that the reversion changes previously reported in malignant phenotypes of HLR60-9 cells and the inhibition of macromolecule biosynthesis mentioned above were associated with the blockade of c-myc gene expression by its antisense RNA.
Li
Y
Y
Department of Biochemistry and Molecular Biology, China-Japan Friendship Institute of Clinical Medical Sciences, Beijing.
Fan
M
M
Zhang
J
J
D002471:Cell Transformation, Neoplastic / Q000235:genetics; D004273:DNA, Neoplasm / Q000096:biosynthesis; D015972:Gene Expression Regulation, Neoplastic; D016259:Genes, myc; D018922:HL-60 Cells / Q000378:metabolism*; D006801:Humans; D009363:Neoplasm Proteins / Q000096:biosynthesis; D010957:Plasmids; D016372:RNA, Antisense / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis*; D012334:RNA, Neoplasm / Q000096:biosynthesis; D014162:Transfection
D004273:DNA, Neoplasm; D009363:Neoplasm Proteins; D016372:RNA, Antisense; D012333:RNA, Messenger; D012334:RNA, Neoplasm