Immunogenicity and protective efficacy of a tuberculosis DNA vaccine.
Tuberculosis is the most widespread and lethal infectious disease affecting humans. Immunization of mice with plasmid DNA constructs encoding one of the secreted components of Mycobacterium tuberculosis, antigen 85 (Ag85), induced substantial humoral and cell-mediated immune responses and conferred significant protection against challenge with live M. tuberculosis and M. bovis bacille Calmette-Guérin (BCG). These results indicate that immunization with DNA encoding a mycobacterial antigen provides an efficient and simple method for generating protective immunity and that this technique may be useful for defining the protective antigens of M. tuberculosis, leading to the development of a more effective vaccine.
Huygen
K
K
Department of Virology, Pasteur Institute, Brussels, Belgium.
Content
J
J
Denis
O
O
Montgomery
D L
DL
Yawman
A M
AM
Deck
R R
RR
DeWitt
C M
CM
Orme
I M
IM
Baldwin
S
S
D'Souza
C
C
Drowart
A
A
Lozes
E
E
Vandenbussche
P
P
Van Vooren
J P
JP
Liu
M A
MA
Ulmer
J B
JB
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000917:Antibody Formation; D000942:Antigens, Bacterial / Q000235:genetics* / Q000276:immunology; D001500:BCG Vaccine / Q000008:administration & dosage / Q000276:immunology*; D016207:Cytokines / Q000276:immunology; D004269:DNA, Bacterial / Q000008:administration & dosage / Q000276:immunology*; D004195:Disease Models, Animal; D007111:Immunity, Cellular; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology; D014376:Tuberculosis / Q000517:prevention & control*; D014407:Tumor Cells, Cultured
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001500:BCG Vaccine; D016207:Cytokines; D004269:DNA, Bacterial; C065036:antigen 85, Mycobacterium bovis