Determining factors for the outcome of peripheral blood progenitor cells harvests.
This is a pilot retrospective study to investigate the factors that may affect the collection of peripheral blood progenitor cells (PBPC). Sixty-nine PBPC harvests in 18 cancer patients (median age 39.5; 8 males and 10 females) were performed during marrow recovery after chemotherapy and hematopoietic growth factors. Median number of nucleated cells (MNC) collected were 13.3 (range 2.3-44.5) x 10(9) per session. Median CFU-GM was 362 colonies (range 63-1,720) per 500,000 MNC. Neither sex, body weight, diagnosis, nor the number of days into leukapheresis was significantly associated with MNC and CFU-GM. Older patients tend to have higher CFU-GM in the PBPC harvests (P = .0437). Higher WBC on the day of harvest is significantly associated with higher yield of MNC after leukapheresis (P < .0001). Patients without any evidence of disease have significantly higher yield of MNC than those having local/distant metastases with or without marrow involvement (P = .0302 and .0446). For patients with metastatic disease, those with bone marrow involvement tend to have higher CFU-GM than those without bone marrow involvement although the difference is not statistically significant (P = .0559). Those patients who have received only one, or three and more chemotherapy regimens have a higher yield of MNC than those who have only two previous chemotherapy regimens (P = .036 and .0324). The mechanism of PBPC mobilization is also discussed. In view of the limited patient number in this study, the results should be confirmed by larger studies.
Keung
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YK
Division of Oncology/Hematology, Texas Tech University, Lubbock 79430, USA.
Cobos
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E
Dunn
D
D
Park
M
M
Dixon
S
S
Wu
K
K
Park
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CH
D000293:Adolescent; D000328:Adult; D002452:Cell Count; D003114:Colony-Forming Units Assay; D005260:Female; D006098:Granulocytes / Q000166:cytology; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D007937:Leukapheresis; D008264:Macrophages / Q000166:cytology; D008297:Male; D008875:Middle Aged; D009369:Neoplasms / Q000097:blood*; D010865:Pilot Projects; D012189:Retrospective Studies; D012307:Risk Factors