title: Effect of engineering Hsp70 copy number on Hsp70 expression and tolerance of ecologically relevant heat shock in larvae and pupae of Drosophila melanogaster.
abstract: To determine how the accumulation of the major Drosophila melanogaster heat-shock protein, Hsp70, affects inducible thermotolerance in larvae and pupae, we have compared two sister strains generated by site-specific homologus recombination. One strain carried 12 extra copies of the Hsp70 gene at a single insertion site (extra-copy strain) and the other carried remnants of the transgene construct but lacked the extra copies of Hsp70 (excision strain). Hsp70 levels in whole-body lysates of larvae and pupae were measured by ELISA with an Hsp70-specific antibody. In both extra-copy and excision strains, Hsp70 was undetectable prior to heat shock. Hsp70 concentrations were higher in the extra-copy strain than in the excision strain at most time points during and after heat shock. Pretreatment (i.e. exposure to 36 degrees C before heat shock) significantly improved thermotolerance, and this improvement was greater and more rapid in larvae and pupae of the extra-copy strain than in those of the excision strain. The experimental conditions resemble thermal regimes actually experienced by Drosophila in the field. Thus, these findings represent the best evidence to date that the amount of a heat-shock protein affects the fitness of a complex animal in the wild.
authors.affiliation: Department of Organismal Biology and Anatomy, University of Chicago, IL 60637, USA.
authors.forename: M E
authors.initials: ME
authors.lastname: Feder
authors.forename: N V
authors.initials: NV
authors.lastname: Cartaño
authors.forename: L
authors.initials: L
authors.lastname: Milos
authors.forename: R A
authors.initials: RA
authors.lastname: Krebs
authors.forename: S L
authors.initials: SL
authors.lastname: Lindquist
mesh_terms: D000818:Animals; D004331:Drosophila melanogaster / Q000235:genetics / Q000502:physiology*; D015870:Gene Expression; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics* / Q000502:physiology*; D006358:Hot Temperature; D007700:Kinetics; D007814:Larva / Q000502:physiology; D015202:Protein Engineering; D011679:Pupa / Q000502:physiology; D011995:Recombination, Genetic
chemical_list: D018840:HSP70 Heat-Shock Proteins