title: Destabilization of simple repetitive DNA sequences by transcription in yeast.
abstract: Simple repetitive DNA sequences in the eukaryotic genome frequently alter in length. In wild-type strains, we find that transcription through a repetitive poly GT tract destabilizes the tract four- to ninefold. In mismatch repair-deficient yeast strains, simple repeats are very unstable. High levels of transcription in such strains destabilize repetitive tracts an additional two- to threefold.
authors.affiliation: Department of Biology, University of North Carolina, Chapel Hill 27599-3280, USA.
authors.forename: M
authors.initials: M
authors.lastname: Wierdl
authors.forename: C N
authors.initials: CN
authors.lastname: Greene
authors.forename: A
authors.initials: A
authors.lastname: Datta
authors.forename: S
authors.initials: S
authors.lastname: Jinks-Robertson
authors.forename: T D
authors.initials: TD
authors.lastname: Petes
mesh_terms: D004249:DNA Damage; D004260:DNA Repair; D004271:DNA, Fungal / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D017422:Sequence Analysis, DNA; D014158:Transcription, Genetic
chemical_list: D004271:DNA, Fungal