title: Crystallization and preliminary X-ray analysis of Escherichia coli methionyl-tRNA(fMet) formyltransferase.
abstract: Methionyl-tRNA(fMet) formyltransferase from Escherichia coli, a monomer of 34kDa, was overexpressed from its cloned gene fmt (Guillon, J.M., Mechulam, Y., Schmitter, J.M., Blanquet, S., and Fayat, G., J. Bacteriol. 174:4294-4301, 1992) and crystallized using ammonium sulphate as precipitant. The crystals are trigonal and have unit cell parameters a = b = 151.0 A, c = 81.8 A. They belong to space group P3(2)21 and diffract to 2.0 A resolution. The structure is being solved by multiple isomorphous replacement.
authors.affiliation: Laboratorie de Biochimie, Unité de Recherche Associée no. 1970 du Centre National de la Recherche Scientifique, Ecole Polytechnique, Palaiseau, France.
authors.forename: E
authors.initials: E
authors.lastname: Schmitt
authors.forename: Y
authors.initials: Y
authors.lastname: Mechulam
authors.forename: M
authors.initials: M
authors.lastname: Ruff
authors.forename: A
authors.initials: A
authors.lastname: Mitschler
authors.forename: D
authors.initials: D
authors.lastname: Moras
authors.forename: S
authors.initials: S
authors.lastname: Blanquet
mesh_terms: D000217:Acyltransferases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D000645:Ammonium Sulfate; D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D019877:Hydroxymethyl and Formyl Transferases
chemical_list: D019877:Hydroxymethyl and Formyl Transferases; C021561:methionyl-tRNA formyltransferase; D000217:Acyltransferases; D000645:Ammonium Sulfate