Detection of Trichinella pseudospiralis antigen in the parenteral phase in experimentally infected mice.
Antigen distribution of T. pseudospiralis in muscles was studied at 10 days, 2,3 and 5 weeks post infection. Immunocytolocalization of antigen in the muscle was performed by peroxidase antiperoxidase (PAP) and immunogold silver staining techniques. Weak positive reaction of the stichosome and the cuticle was observed at 10 days post-infection which increased to attain its highest level in the fully infective stage larvae 3 and 5 weeks post infection. The intensity of staining reaction in the cytoplasm and nucleoplasm of infected host cell and surrounding tissue was highest at 5 weeks p.i. The small nuclei of the host cells and the inflammatory cells were unstained.
Boulos
L M
LM
Department of Parasitology, University of Alexandria, Egypt.
El-Mansoury
S T
ST
Hegazy
I H
IH
El-Gohari
S H
SH
Ebrahim
E R
ER
D000818:Animals; D000947:Antigens, Helminth / Q000032:analysis*; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D016253:Microscopy, Immunoelectron; D018482:Muscle, Skeletal / Q000469:parasitology / Q000473:pathology*; D011817:Rabbits / Q000276:immunology; D014234:Trichinella / Q000302:isolation & purification*; D014235:Trichinellosis / Q000473:pathology*
D000947:Antigens, Helminth