title: Nucleotide variation at the Gpdh locus in the genus Drosophila.
abstract: The Gpdh locus was sequenced in a broad range of Drosophila species. In contrast to the extreme evolutionary constraint seen at the amino acid level, the synonymous sites evolve at rates comparable to those of other genes. Gpdh nucleotide sequences were used to infer a phylogenetic tree, and the relationships among the species of the obscura group were examined in detail. A survey of nucleotide polymorphism within D. pseudoobscura revealed no amino acid variation in this species. Applying a modified McDonald-Kreitman test, the amino acid divergence between species in the obscura group does not appear to be excessive, implying that drift is adequate to explain the patterns of amino acid change at this locus. In addition, the level of polymorphism at the Gpdh locus in D. pseudoobscura is comparable to that found at other loci, as determined by a Hudson-Kreitman-Aguadé test. Thus, the pattern of nucleotide variation within and between species at the Gpdh locus is consistent with a neutral model.
authors.affiliation: Museum of Comparative Zoology, Harvard University, Cambridge, Massachusetts 02138, USA. wells@lotka.stanford.edu
authors.forename: R S
authors.initials: RS
authors.lastname: Wells
mesh_terms: D000465:Algorithms; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004330:Drosophila / Q000201:enzymology / Q000235:genetics*; D005091:Exons; D017344:Genes, Insect; D014644:Genetic Variation; D015698:Genomic Library; D005993:Glycerolphosphate Dehydrogenase / Q000235:genetics*; D008957:Models, Genetic; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D017384:Sequence Deletion; D013045:Species Specificity
chemical_list: D005993:Glycerolphosphate Dehydrogenase