title: Estradiol influences p53 expression in a human endometrial adenocarcinoma heterotransplanted into nude mice.
abstract: The influence of different estradiol concentrations on the expression of the p53 suppressor gene and on cell kinetics was examined by semiquantitative analysis of protein and bromodeoxyuridine labelling in a human endometrial adenocarcinoma grown in nude mice. We found that increasing the circulating estradiol increases (p = 0.001), and decreasing the hormone value decreases (p = 0.001) the expression of p53 in this tumor. The number of cells in the G1/G0 phase of the cell cycle was significantly higher (p = 0.03), and the number of cells in the G2/M phase was significantly lower (p = 0.01) in tumors grown in estradiol-treated mice than in tumors obtained from the nontreated group. Changes in p53 expression may possibly be explained by either altered transcription activity of the gene or increased half-life of the protein. Our results suggest an important role of estradiol in the progression of estrogen receptor (ER) positive human endometrial adenocarcinomas.
authors.affiliation: Division of Gynaecological Oncology, Sahlgrenska University Hospital, Gothenburg, Sweden.
authors.forename: G
authors.initials: G
authors.lastname: Horvath
authors.forename: G
authors.initials: G
authors.lastname: Leser
authors.forename: U
authors.initials: U
authors.lastname: Delle
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism*; D000818:Animals; D002455:Cell Division; D016889:Endometrial Neoplasms / Q000378:metabolism*; D004958:Estradiol / Q000031:analogs & derivatives* / Q000494:pharmacology; D045166:Estradiol Congeners / Q000494:pharmacology*; D005260:Female; D006801:Humans; D007700:Kinetics; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis*
chemical_list: D045166:Estradiol Congeners; D016159:Tumor Suppressor Protein p53; D004958:Estradiol; C008958:polyestradiol phosphate