title: Regulation of immunoreactive activin A secretion from cultured rat anterior pituitary cells.
abstract: Immunoreactive activin A (ir-activin A) release from cultured rat anterior pituitary cells was examined by measuring ir-activin A in culture medium by a specific radioimmunoassay. Ir-activin A release into the medium increased over 1-18 days, and reached a maximal level at 12-15 days. The basal levels of ir-activin A in the culture media were 0.70 +/- 0.10 (mean +/- SD), 1.30 +/- 0.36 and 1.83 +/- 0.44 ng/10(6) cells, when cultured for 6 days with 0, 2 and 10% fetal calf serum, respectively. LHRH induced an approximate 1.4-fold increase in ir-activin A release in contrast to a 40-60% inhibition with FSH, but LH did not affect the activin A release. In the presence of 12-o-tetradecanoylphorbol acetate (TPA), iractivin A release was enhanced, but no significant effect was induced by forskolin. Activin A was distinctly immunostained in cultured rat anterior pituitary cells. These results suggested that activin A release from the pituitary is modified by FSH and LHRH, and that the activation of protein kinase C may be involved in the action of LHRH.
authors.affiliation: First Department of Internal Medicine, University of Tokushima, Japan.
authors.forename: Z H
authors.initials: ZH
authors.lastname: Liu
authors.forename: Y
authors.initials: Y
authors.lastname: Shintani
authors.forename: M
authors.initials: M
authors.lastname: Wakatsuki
authors.forename: Y
authors.initials: Y
authors.lastname: Sakamoto
authors.forename: K
authors.initials: K
authors.lastname: Harada
authors.forename: C Y
authors.initials: CY
authors.lastname: Zhang
authors.forename: S
authors.initials: S
authors.lastname: Saito
mesh_terms: D028341:Activins; D000818:Animals; D002478:Cells, Cultured; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D007987:Gonadotropin-Releasing Hormone / Q000494:pharmacology; D006801:Humans; D007150:Immunohistochemistry; D007265:Inhibins / Q000378:metabolism*; D007700:Kinetics; D007986:Luteinizing Hormone / Q000494:pharmacology; D010903:Pituitary Gland, Anterior / Q000378:metabolism*; D011493:Protein Kinase C / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: D028341:Activins; D007987:Gonadotropin-Releasing Hormone; D007265:Inhibins; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate