title: Detection of HHV-8/KSHV DNA sequences in AIDS-associated extranodal lymphoid malignancies.
abstract: Herpesvirus-like DNA sequences have been identified in a high proportion of both AIDS-associated and classical Kaposi's sarcoma, and in a small percentage of AIDS-associated malignant lymphomas. To determine the extent of involvement of this new agent designated HHV-8 (human herpesvirus type 8) or KSHV (Kaposi's sarcoma-associated herpesvirus) in human malignant lymphomas, we analyzed 24 AIDS-associated lymphoid malignancies and 100 non-AIDS-associated lymphomas by PCR and Southern blot analysis. Three of 24 lymphoid malignancies from patients with AIDS demonstrated HHV-8 sequences by Southern blot and PCR analyses. The fourth was positive by PCR only. None of the non-AIDS-associated lymphomas contained HHV-8 sequences. All three Southern blot positive samples were derived from extranodal regions, two from pleural effusions, and one from a soft tissue mass in the thigh. This latter patient initially presented with a pleural effusion. The fourth PCR positive but Southern blot negative tumor was from a gingival lymphoma in a patient with a history of Kaposi's sarcoma. All tumors positive for HHV-8 were also positive for EBV. These results confirm a recent report that this novel herpesvirus may play a role in AIDS-associated lymphomas especially in those with body cavity presentation.
authors.affiliation: Laboratory of Pathology, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.
authors.forename: T
authors.initials: T
authors.lastname: Otsuki
authors.forename: S
authors.initials: S
authors.lastname: Kumar
authors.forename: B
authors.initials: B
authors.lastname: Ensoli
authors.forename: D W
authors.initials: DW
authors.lastname: Kingma
authors.forename: T
authors.initials: T
authors.lastname: Yano
authors.forename: M
authors.initials: M
authors.lastname: Stetler-Stevenson
authors.forename: E S
authors.initials: ES
authors.lastname: Jaffe
authors.forename: M
authors.initials: M
authors.lastname: Raffeld
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000150:complications*; D000328:Adult; D001483:Base Sequence; D015139:Blotting, Southern; D017931:DNA Primers; D015342:DNA Probes; D004279:DNA, Viral / Q000032:analysis* / Q000235:genetics; D018023:HIV Seronegativity; D006564:Herpesviridae / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008223:Lymphoma / Q000401:mortality / Q000473:pathology / Q000821:virology*; D016483:Lymphoma, AIDS-Related / Q000401:mortality / Q000473:pathology / Q000821:virology*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012514:Sarcoma, Kaposi / Q000209:etiology / Q000821:virology*; D016019:Survival Analysis
chemical_list: D017931:DNA Primers; D015342:DNA Probes; D004279:DNA, Viral