Predictive testing for respiratory sensitization in the mouse.
Attempts to develop predictive test methods for the identification of chemical respiratory allergens have to date focused almost exclusively on the guinea pig. In recent years there has, however, been a growing interest in the mouse as a model for examination of sensitization potential. In this article two alternative approaches to the toxicological investigation of respiratory sensitization are described. Both are based on an understanding of the nature of immune responses induced in mice by chemical allergens. The mouse IgE test seeks to identify chemicals capable of causing allergic sensitization of the respiratory tract as a function of induced increases in the serum concentration of IgE. The second approach, cytokine fingerprinting, makes use of the observation that chemical allergens of different types provoke in mice qualitatively divergent immune responses characterized by discrete cytokine secretion profiles.
Kimber
I
I
Zeneca Central Toxicology Laboratory, Macclesfield, Cheshire, UK.
Hilton
J
J
Basketter
D A
DA
Dearman
R J
RJ
D000485:Allergens / Q000633:toxicity*; D000818:Animals; D016207:Cytokines / Q000032:analysis; D004195:Disease Models, Animal; D008198:Lymph Nodes / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D012130:Respiratory Hypersensitivity / Q000139:chemically induced* / Q000276:immunology; D018675:Toxicity Tests / Q000379:methods*
D000485:Allergens; D016207:Cytokines