title: The structure of recombinant human annexin VI in crystals and membrane-bound.
abstract: The crystal structure of calcium-free recombinant human annexin VI was solved at a resolution of 3.2 A by using the annexin I model for Patterson search and refined to an R-factor of 19.0%. The molecule consists of two similar halves closely resembling annexin I connected by an alpha-helical segment and arranged perpendicular to each other. The calcium and membrane binding sites assigned by structural homology are therefore not located in the same plane. Analysis of the membrane-bound form of annexin VI by electron microscopy shows the two halves of the molecule coplanar with the membrane, but oriented differently to the crystal structure and suggesting a flexible arrangement. Ion channel activity has been found for annexin VI and the half molecules by electrophysiological experiments.
authors.affiliation: Max-Planck-Institut für Biochemie, Abt. Strukturforschung, Martinsried, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Benz
authors.forename: A
authors.initials: A
authors.lastname: Bergner
authors.forename: A
authors.initials: A
authors.lastname: Hofmann
authors.forename: P
authors.initials: P
authors.lastname: Demange
authors.forename: P
authors.initials: P
authors.lastname: Göttig
authors.forename: S
authors.initials: S
authors.lastname: Liemann
authors.forename: R
authors.initials: R
authors.lastname: Huber
authors.forename: D
authors.initials: D
authors.lastname: Voges
mesh_terms: D017317:Annexin A6 / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure; D001665:Binding Sites; D002118:Calcium / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D003460:Crystallization; D018360:Crystallography, X-Ray; D006801:Humans; D007473:Ion Channels / Q000378:metabolism; D008854:Microscopy, Electron; D008958:Models, Molecular; D018408:Patch-Clamp Techniques; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry
chemical_list: D017317:Annexin A6; D007473:Ion Channels; D011994:Recombinant Proteins; D002118:Calcium