L-2-Oxothiazolidine-4-carboxylate and N-acetylcysteine as precursors of intracellular glutathione in human peritoneal mesothelial cells.
L-2-Oxothiazolidine-4-carboxylate and N-acetylcysteine as substrates for intracellular glutathione in human peritoneal mesothelial cells were tested. Both substances at concentrations of 0.01 mM and higher augmented the level of glutathione in mesothelial cells. L-2-Oxothiazolidine-4-carboxylate had a milder but more stable effect than N-acetylcysteine. Cells with increased concentrations of the intracellular glutathione were more resistant to injury by free radicals. When used at higher concentrations (> 1 mM), both substances became cytostatic to mesothelial cells as evidenced by growth inhibition.
Breborowicz
A
A
Department of Pathophysiology, Medical School, Poznan, Poland.
Patrikarea
A
A
Martis
L
L
Oreopoulos
D G
DG
D000111:Acetylcysteine / Q000494:pharmacology*; D002478:Cells, Cultured; D004847:Epithelial Cells; D004848:Epithelium / Q000187:drug effects / Q000378:metabolism; D016166:Free Radical Scavengers / Q000494:pharmacology*; D005609:Free Radicals; D005978:Glutathione / Q000378:metabolism*; D006801:Humans; D010537:Peritoneum / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D011498:Protein Precursors / Q000378:metabolism*; D011761:Pyrrolidonecarboxylic Acid; D013844:Thiazoles / Q000494:pharmacology*; D053778:Thiazolidines
D016166:Free Radical Scavengers; D005609:Free Radicals; D011498:Protein Precursors; D013844:Thiazoles; D053778:Thiazolidines; D005978:Glutathione; D011761:Pyrrolidonecarboxylic Acid; D000111:Acetylcysteine; C029536:2-oxothiazolidine-4-carboxylic acid