Oxidative damage to lens membranes induced by metal-catalyzed systems.
The oxidative effect of hydrogen peroxide, ascorbic acid and glucose in the presence of transition metals was studied. Incubation of bovine lens membranes with hydrogen peroxide/metal, ascorbic acid/metal and glucose/metal systems resulted in a significant augmentation of thiobarbituric acid-reactive substance content in the membranes. Presence of alpha-tocopherol decreased the extent of oxidative damage. This oxidation was found to be mediated by hydroxyl radicals. Dependence of hydroxyl radical generation upon the buffer used has been observed. In all experiments Cu(II) ions exhibited a higher efficiency compared to Fe(II) ions.
Yugay
M T
MT
Department of Ophthalmology and Visual Sciences, Coimbra University Hospitals, Portugal.
Pereira
P C
PC
Leiria
F
F
Mota
M C
MC
D000818:Animals; D001205:Ascorbic Acid / Q000737:chemistry; D002384:Catalysis; D002417:Cattle; D002462:Cell Membrane / Q000737:chemistry; D005947:Glucose / Q000737:chemistry; D006861:Hydrogen Peroxide / Q000737:chemistry; D017665:Hydroxyl Radical / Q000737:chemistry; D007908:Lens, Crystalline / Q000737:chemistry*; D008054:Lipid Peroxides / Q000737:chemistry*; D008670:Metals / Q000737:chemistry*; D010084:Oxidation-Reduction
D008054:Lipid Peroxides; D008670:Metals; D017665:Hydroxyl Radical; D006861:Hydrogen Peroxide; D005947:Glucose; D001205:Ascorbic Acid