O-acetylated xyloglucan in extracellular polysaccharides from cell-suspension cultures of Mentha.
Extracellular polysaccharide produced by suspension-cultured Mentha cells consisted of 50% neutral sugars, 32% uronic acid and 10% of protein. The ammonium oxalate-soluble fraction of this polysaccharide contained 30% hemicellulose and 70% pectic substances. The purified hemicellulose contained xylose, glucose, arabinose, galactose, mannose and fucose residues in a molar ratio of 41.6:31.3:13.1:11.1:1.3:1.6. It was identified as a xyloglucan from its neutral sugar composition and by methylation analysis and cellulase treatment: the principal neutral sugar was arabinose. The presence of O-acetyl residues was confirmed with 1H NMR, 13C NMR and GC-mass spectrometry. The total acetyl content in the polysaccharide was 4%. The point of attachment of the O-acetyl residue was shown to be at position 6 of the galactosyl residue.
Maruyama
K
K
Oleochemical Research Laboratory, Japan.
Goto
C
C
Numata
M
M
Suzuki
T
T
Nakagawa
Y
Y
Hoshino
T
T
Uchiyama
T
T
D000107:Acetylation; D002236:Carbohydrate Conformation; D002241:Carbohydrates / Q000032:analysis; D002478:Cells, Cultured; D002480:Cellulase; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D004590:Electrophoresis, Paper; D008401:Gas Chromatography-Mass Spectrometry; D005936:Glucans; D006868:Hydrolysis; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D059828:Plant Cells; D010944:Plants / Q000378:metabolism*; D011134:Polysaccharides / Q000096:biosynthesis* / Q000737:chemistry* / Q000302:isolation & purification; D014990:Xylans
D002241:Carbohydrates; D005936:Glucans; D011134:Polysaccharides; D014990:Xylans; C029353:xyloglucan; D002480:Cellulase