title: Pyridine derivatives stimulate phosphatidylcholine secretion in primary cultures of rat type II pneumocytes.
abstract: We have examined the effects of pyridine derivatives on phosphatidylcholine secretion in primary cultures of rat type II pneumocytes. Of 12 pyridine derivatives, 4-aminopyridine, 4-dimethylaminopyridine and 4-pyrolidinopyridine had a stimulatory effect on phosphatidylcholine secretion, whereas other derivatives had little effect. The stimulatory effect of 4-aminopyridine was concentration- and time-dependent, and was inhibited by the acetoxymethyl ester of 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid (3 microM), an intracellular Ca2+ chelator. In addition, the stimulatory effect of 4-aminopyridine was suppressed by W-7(N-(6-aminohexyl)-5-chloro-1-napthalene-sulphonamide)(10 microM), a calmodulin inhibitor, and sphingosine (10 microM) and staurosporine (0-1 microM), protein kinase C inhibitors. These results indicate that several pyridine derivatives stimulate phosphatidylcholine secretion in type II pneumocytes.
authors.affiliation: Department of Pharmacological Sciences, Faculty of Pharmaceutical Sciences, Kumamoto University, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Kai
authors.forename: K
authors.initials: K
authors.lastname: Murahara
authors.forename: Y
authors.initials: Y
authors.lastname: Isohama
authors.forename: K
authors.initials: K
authors.lastname: Takahama
authors.forename: Y
authors.initials: Y
authors.lastname: Oda
authors.forename: I
authors.initials: I
authors.lastname: Hamamura
authors.forename: K
authors.initials: K
authors.lastname: Yoshitake
authors.forename: T
authors.initials: T
authors.lastname: Miyata
mesh_terms: D015761:4-Aminopyridine / Q000031:analogs & derivatives* / Q000378:metabolism / Q000633:toxicity*; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D002614:Chelating Agents / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004533:Egtazic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008168:Lung / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D008297:Male; D010713:Phosphatidylcholines / Q000378:metabolism*; D011725:Pyridines / Q000378:metabolism / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar; D013047:Specific Pathogen-Free Organisms; D013329:Structure-Activity Relationship; D013449:Sulfonamides / Q000494:pharmacology; D014665:Vasodilator Agents / Q000494:pharmacology
chemical_list: D002614:Chelating Agents; D010713:Phosphatidylcholines; D011725:Pyridines; D013449:Sulfonamides; D014665:Vasodilator Agents; C070379:1,2-bis(2-aminophenoxy)ethane N,N,N',N'-tetraacetic acid acetoxymethyl ester; C021249:4-pyrrolidinopyridine; D004533:Egtazic Acid; C017967:W 7; D015761:4-Aminopyridine; D007770:L-Lactate Dehydrogenase; C003885:4-dimethylaminopyridine; D002118:Calcium