A metastatic neuroblastoma model in SCID mice.
Neuroblastoma (NB), a neural crest derived tumor in children, shows a characteristic pattern of dissemination that includes adrenal glands, local lymph nodes, bone, liver, skin, and bone marrow. We have reconstructed a similar metastatic pattern in SCID mice following tail vein injection of human NB cells. HTLA230, an NB cell line isolated from a patient with advanced disease, and its NGF receptor (trkA) expressing derivative (18-10) cells, consistently disseminated to the liver, the adrenal gland, and the bone marrow, but not the lungs. Metastases in the different organs showed a characteristic hemorrhagic histopathology, and tumors in the bone marrow presented as syncytia-like cell aggregates, typically seen in patients. Cell lines reestablished from 18-10 derived liver and bone marrow metastases maintained their cellular morphology, growth behavior, N-myc overexpression, trkA expression, and functionally responded to NGF treatment, leading to growth arrest and neurite outgrowth. Hence circulating human NB cells in SCID mice show a similar organ-specific metastatic potential as seen in patients, independent of trkA expression.
Bogenmann
E
E
Division of Hematology/Oncology, Childrens Hospital Los Angeles, CA 90027, USA. Ebogenmann%smtpgate@chlais.usc.edu
D000310:Adrenal Gland Neoplasms / Q000556:secondary; D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D008114:Liver Neoplasms, Experimental / Q000556:secondary; D051379:Mice; D016513:Mice, SCID; D009362:Neoplasm Metastasis; D009368:Neoplasm Transplantation; D009447:Neuroblastoma / Q000473:pathology* / Q000556:secondary* / Q000648:ultrastructure; D009928:Organ Specificity; D010051:Ovarian Neoplasms / Q000556:secondary; D017475:Receptors, Nerve Growth Factor / Q000096:biosynthesis / Q000235:genetics; D014162:Transfection
D017475:Receptors, Nerve Growth Factor