Human herpesvirus 6 induces IL-8 gene expression in human hepatoma cell line, Hep G2.
The infectivity of human herpesvirus 6 (HHV-6) in a human hepatoma cell line, Hep G2 cells, and the effect of HHV-6 on production of inflammatory cytokines in these cells were examined to analyze pathogenesis of HHV-6 in the liver. We demonstrated that Hep G2 cells were susceptible to infection with HHV-6, and produced infectious virus. Moreover, infection of Hep G2 cells by HHV-6 induced the expression of IL-8 mRNA, but not IL-1 beta. The effect on induction of IL-8 gene expression was observed only in Hep G2 cells infected with infectious virus, whereas both heat-inactivated HHV-6 and UV-irradiated HHV-6 did not change the IL-8 mRNA level in these cells. These data suggest that HHV-6 may induce the cytokine-mediated inflammatory response by infecting liver cells, which could result in liver dysfunction in vivo.
Inagi
R
R
Department of Microbiology, Osaka University Medical School, Japan.
Guntapong
R
R
Nakao
M
M
Ishino
Y
Y
Kawanishi
K
K
Isegawa
Y
Y
Yamanishi
K
K
D000956:Antigens, Viral / Q000032:analysis; D001483:Base Sequence; D006528:Carcinoma, Hepatocellular; D017931:DNA Primers; D005786:Gene Expression Regulation; D006361:Heating; D015654:Herpesvirus 6, Human / Q000302:isolation & purification / Q000502:physiology*; D006801:Humans; D007375:Interleukin-1 / Q000235:genetics; D016209:Interleukin-8 / Q000235:genetics*; D008969:Molecular Sequence Data; D012333:RNA, Messenger; D014407:Tumor Cells, Cultured; D014466:Ultraviolet Rays; D015854:Up-Regulation; D014779:Virus Replication
D000956:Antigens, Viral; D017931:DNA Primers; D007375:Interleukin-1; D016209:Interleukin-8; D012333:RNA, Messenger