Plant virus movement protein dynamics probed with a GFP-protein fusion.
A genetic fusion between the gene encoding green fluorescent protein (GFP) from the jellyfish Aequorea victoria, with that of the Ob-tobamovirus movement protein (MP) resulted in the expression of a fluorescent fusion protein (MP::GFP) that was fully biologically active in mediating the cell-to-cell spread of the Ob-virus. The MP::GFP fusion was used to follow in planta the subcellular trafficking of MP. GFP-tagged MP was transiently expressed and found to be associated with several subcellular compartments and structures including trans-wall structures, presumably plasmodesmata, and filament structures. The MP::GFP fusion can be used to monitor MP association with host proteins and structures, and for the isolation of interacting host components.
Epel
B L
BL
Department of Cell Biology, Scripps Research Institute, La Jolla, CA 92037, USA.
Padgett
H S
HS
Heinlein
M
M
Beachy
R N
RN
D000818:Animals; D003001:Cloning, Molecular; D049452:Green Fluorescent Proteins; D007700:Kinetics; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism*; D018515:Plant Leaves / Q000821:virology; D053830:Plant Viral Movement Proteins; D010947:Plants, Toxic; D011523:Protoplasts / Q000821:virology; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D037401:Scyphozoa; D014026:Nicotiana / Q000821:virology; D017898:Tobamovirus / Q000235:genetics / Q000378:metabolism*; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
D008164:Luminescent Proteins; D053830:Plant Viral Movement Proteins; D011993:Recombinant Fusion Proteins; D014764:Viral Proteins; D049452:Green Fluorescent Proteins