title: Site-directed mutagenesis of double-stranded plasmids, domain substitution, and marker rescue by comutagenesis of restriction enzyme sites.
authors.affiliation: Department of Cancer Biology, Harvard University School of Public Health, Boston, MA, USA.
authors.forename: J A
authors.initials: JA
authors.lastname: Nickoloff
authors.forename: W P
authors.initials: WP
authors.lastname: Deng
authors.forename: E M
authors.initials: EM
authors.lastname: Miller
authors.forename: F A
authors.initials: FA
authors.lastname: Ray
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics / Q000378:metabolism; D004262:DNA Restriction Enzymes / Q000235:genetics*; D005819:Genetic Markers; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D010766:Phosphorylation; D010957:Plasmids / Q000235:genetics*; D016133:Polymerase Chain Reaction; D014162:Transfection / Q000379:methods
chemical_list: D017931:DNA Primers; D005819:Genetic Markers; D004247:DNA; D004262:DNA Restriction Enzymes