title: [Detection of p53 mutation using PCR-SSCP silver staining method].
abstract: A simple and sensitive technique to detect point mutation of the p53 gene using silver staining method for single-strand conformation polymorphism analysis of polymerase chain reaction (PCR-SSCP) is reported. In this study, the aberrations of the p53 gene (exon 6-8) in clinical specimens of primary cervical carcinoma were examined. Of 27 tumor tissues 2 samples showed mutations of p53 exon 7. Absence of abnormal bands in the p53 exon 6-8 in all ten normal cervical tissues. It appears that inactivation of p53 gene by point mutation is infrequent in clinical samples of human cervical carcinomas.
authors.affiliation: Institute of Basic Medical Sciences, CAMS, Beijing.
authors.forename: W
authors.initials: W
authors.lastname: Zhao
authors.forename: J
authors.initials: J
authors.lastname: Si
authors.forename: K
authors.initials: K
authors.lastname: Li
authors.forename: X
authors.initials: X
authors.lastname: Zhang
authors.forename: G
authors.initials: G
authors.lastname: Song
mesh_terms: D001483:Base Sequence; D004273:DNA, Neoplasm / Q000235:genetics; D005091:Exons; D005260:Female; D016158:Genes, p53; D006801:Humans; D008969:Molecular Sequence Data; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D016622:Silver Staining; D002583:Uterine Cervical Neoplasms / Q000235:genetics*
chemical_list: D004273:DNA, Neoplasm