Effect of antituberculous drugs, isoniazid, pyrazinamide and rifampicin, on chemiluminescence of the human polymorphonuclear leukocytes.
Luminol-dependent chemiluminescence (CL) was used to assay the effect of three antituberculous drugs [isoniazid (INH), pyrazinamide (PZA), rifampicin (RM)] on the production of reactive oxygen species (ROS) by polymorphonuclear leukocytes (PMN) isolated from blood of 5 healthy donors. Drugs were added directly to the medium in concentrations; INH 5 micrograms/ml, PZA 40 micrograms/ml, RM 7 micrograms/ml. These concentrations correspond to peak serum concentrations after usual doses. RM was found to inhibit FMLP (N-formyl-methionyl-leucyl -phenylalanine)-induced CL response of PMN, PZA did not influence the metabolic activity of neutrophils, and INH stimulated PMN CL.
Demkow
U
U
National Research Institute of Tuberculosis and Lung Diseases, Warsaw, Poland.
Małkowska-Zwierz
W
W
Rogala
E
E
Skopińska-Rózewska
E
E
Lewandowski
Z
Z
D000995:Antitubercular Agents / Q000494:pharmacology*; D006801:Humans; D007538:Isoniazid / Q000494:pharmacology*; D008163:Luminescent Measurements; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism; D011718:Pyrazinamide / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D012293:Rifampin / Q000494:pharmacology*
D000995:Antitubercular Agents; D017382:Reactive Oxygen Species; D011718:Pyrazinamide; D007538:Isoniazid; D012293:Rifampin