Synergistic inhibitory effect of enviroxime and disoxaril on poliovirus type 1 replication.
The effects of enviroxime, disoxaril and ribavirin in pair combinations on poliovirus type 1 (Mahoney) replication in FL cells were tested. Beforehand, the fifty percent inhibitory concentration (IC50) was determined for each compound alone: enviroxime - 0.2 mumol/1, disoxaril - 0.3 mumol/1, ribavirin - 3 mumol/1. Combining enviroxime with disoxaril resulted in synergistic interaction, while combinations with ribavirin were markedly antagonistic. Enviroxime-and disoxaril-resistant poliovirus mutants appeared following 10 and 2 consecutive passages in FL cells, respectively. No cross-resistance was observed between these mutants towards disoxaril and enviroxime, respectively.
Nikolaeva
L
L
Department of Virology, Bulgarian Academy of Sciences, Sofia, Bulgaria.
Galabov
A S
AS
D000998:Antiviral Agents / Q000494:pharmacology*; D001562:Benzimidazoles / Q000494:pharmacology*; D002460:Cell Line; D004351:Drug Resistance; D004357:Drug Synergism; D006801:Humans; D007555:Isoxazoles / Q000494:pharmacology*; D010091:Oximes; D017955:Poliovirus / Q000187:drug effects* / Q000502:physiology; D012254:Ribavirin / Q000494:pharmacology*; D013449:Sulfonamides; D014779:Virus Replication / Q000187:drug effects
D000998:Antiviral Agents; D001562:Benzimidazoles; D007555:Isoxazoles; D010091:Oximes; D013449:Sulfonamides; D012254:Ribavirin; C045978:disoxaril; C021595:viroxime