Antisense oligonucleotide to NOR-1, a novel orphan nuclear receptor, induces migration and neurite extension of cultured forebrain cells.
We previously identified a novel orphan nuclear receptor referred to as NOR-1 from rat forebrain cells. This study examined the role of NOR-1 in primary cultured forebrain cells by selectively inhibiting NOR-1 expression by addition of antisense oligonucleotide to the culture media. Treating cells with the antisense oligomer resulted in the following dramatic morphological changes: (i) cell migration, (ii) extension of processes, and (iii) formation of cellular aggregates. Immunocytochemistry for microtubule-associated protein 2 revealed that the processes were filled with neurites growing from neuronal cells. These findings suggest that NOR-1 may be involved in the molecular mechanisms regulating neural differentiation.
Ohkura
N
N
Institute of Biomedical Science, TERUMO R&D Center, Kanagawa, Japan.
Hijikuro
M
M
Miki
K
K
D000818:Animals; D001483:Base Sequence; D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D004268:DNA-Binding Proteins / Q000235:genetics*; D004622:Embryo, Mammalian; D005260:Female; D008869:Microtubule-Associated Proteins / Q000032:analysis; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics*; D016501:Neurites / Q000187:drug effects / Q000502:physiology*; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D016376:Oligonucleotides, Antisense / Q000494:pharmacology*; D011247:Pregnancy; D016548:Prosencephalon / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D011987:Receptors, Steroid / Q000235:genetics; D013873:Thionucleotides
D004268:DNA-Binding Proteins; D008869:Microtubule-Associated Proteins; D009419:Nerve Tissue Proteins; C091333:Nr4a3 protein, rat; D016376:Oligonucleotides, Antisense; D011987:Receptors, Steroid; D013873:Thionucleotides