The effect of co-administered drugs on oxaprozin binding to human serum albumin.
The binding of the non-steroidal anti-inflammatory drug oxaprozin to human serum albumin was studied by bioaffinity high-performance liquid chromatography using a column based on immobilized human serum albumin. Displacement studies using marker compounds for the major drug binding sites showed that oxaprozin has a high affinity for the benzodiazepine/indole site and binds to the warfarin site but with a significantly lower affinity. Biochromatography and ultrafiltration techniques were used to screen for possible competition and allosteric interactions between oxaprozin and potential co-administered drugs including non-steroidal anti-inflammatory drugs, antipyretics, hypoglycaemics, inhibitors of angiotensin-converting enzyme, anaesthetics, metal ions and anticancer agents. Competition occurred mainly with drugs bound at the benzodiazepine site (benzodiazepines, various non-steroidal anti-inflammatories).
Aubry
A F
AF
Department of Oncology, McGill University, Montreal, Quebec, Canada.
Markoglou
N
N
Adams
M H
MH
Longstreth
J
J
Wainer
I W
IW
D000806:Angiotensin-Converting Enzyme Inhibitors / Q000378:metabolism; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000378:metabolism*; D001665:Binding Sites; D004347:Drug Interactions; D006801:Humans; D007004:Hypoglycemic Agents / Q000378:metabolism; D000077431:Oxaprozin; D011422:Propionates / Q000378:metabolism*; D011485:Protein Binding; D012709:Serum Albumin / Q000378:metabolism*; D013629:Tamoxifen / Q000378:metabolism; D014859:Warfarin / Q000378:metabolism
D000806:Angiotensin-Converting Enzyme Inhibitors; D000894:Anti-Inflammatory Agents, Non-Steroidal; D007004:Hypoglycemic Agents; D011422:Propionates; D012709:Serum Albumin; D013629:Tamoxifen; D014859:Warfarin; D000077431:Oxaprozin