title: Active transport activities of free B-6 vitamers in various yeast strains.
abstract: Active transport activities of free B-6 vitamers in 35 strains of 8 genera of yeast were measured by isocratic reverse-phase HPLC. Many but not all strains transported pyridoxamine and/or pyridoxine. The active transport activities in some yeast strains tested were completely inhibited by amiloride (0.5 mM). In contrast to cells so far studied, yeast cells showed a novel character in metabolism of accumulated B-6 vitamers: the phosphorylation of the free B-6 vitamers was regulated at a low level. There was no apparent correlation between the presence of the active transport activity in yeasts and the requirement of vitamin B-6 for their growth.
authors.affiliation: Department of Bioresources Science, Faculty of Agriculture, Kochi University, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Yagi
authors.forename: Y
authors.initials: Y
authors.lastname: Kim
authors.forename: Y
authors.initials: Y
authors.lastname: Hiraoka
authors.forename: A
authors.initials: A
authors.lastname: Tanouchi
authors.forename: T
authors.initials: T
authors.lastname: Yamamoto
authors.forename: S
authors.initials: S
authors.lastname: Yamamoto
mesh_terms: D000584:Amiloride / Q000494:pharmacology; D001693:Biological Transport, Active / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D004232:Diuretics / Q000494:pharmacology; D010766:Phosphorylation; D011730:Pyridoxal / Q000378:metabolism*; D011733:Pyridoxamine / Q000378:metabolism*; D011736:Pyridoxine / Q000378:metabolism*; D013045:Species Specificity; D015003:Yeasts / Q000187:drug effects / Q000378:metabolism*
chemical_list: D004232:Diuretics; D011730:Pyridoxal; D011733:Pyridoxamine; D000584:Amiloride; D011736:Pyridoxine