An in vitro perfusion model for the determination of absorption properties of drugs in isolated rat small intestine.
A two-compartment model was developed to perform in vitro permeability studies of drugs. Preparations of surviving small intestine are continuously perfused under permanent oxygenation. The apparatus especially allows the usage of variable volumes of donor and adaptable length of intestinal segments ranging from 3 to 20 cm. Both the mucosal and the serosal side of the apparatus are open for sample collection and additional instrumentation. Due to the construction the use of high-surface activity compounds is possible. Data may be derived as absorption rates (micrograms.cm-1.min-1) and concentration increases vs. time profiles.
Norta
M
M
Institut für Pharmazie, Humboldt-Universität zu Berlin.
Schöpke
T
T
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D066298:In Vitro Techniques; D007408:Intestinal Absorption / Q000502:physiology*; D007421:Intestine, Small / Q000098:blood supply* / Q000378:metabolism*; D008297:Male; D008954:Models, Biological; D010477:Perfusion; D010599:Pharmacokinetics; D051381:Rats; D017208:Rats, Wistar; D012039:Regional Blood Flow