title: Morphological and hemostatic changes in rats with abdominal arterial prosthesis.
abstract: We evaluated the changes over time in hemostatic factors during ongoing arterial thrombosis in rats, as induced by a loop-shaped aortic prosthesis. Moreover, we investigated this condition by inspecting in parallel local thrombus growth, systemic vascular prostacyclin and t-PA production. One minute after loop insertion, activated platelets spread on the internal surface of the prosthesis and 24 hrs later numerous platelet aggregates supported by a fibrin network could be observed. However, no evidence for platelet activation could be concomitantly found in peripheral blood. A sustained increased in PGI2 formation was detected together with a progressive increase in plasma fibrinolytic activity during thrombus growth. The levels of fibrinogen as well as antithrombin III (ATIII) and heparin cofactor II (HCII) activities were steadily increased in loop-bearing animals. In conclusion, the dynamic phases of thrombus formation, in an aortic prosthesis, produce changes in vascular function and in hemostatic factors at the level of systemic blood.
authors.affiliation: Laboratory of Thrombosis Pharmacology, Istituto di Ricerche Farmacologiche Mario Negri, Consorzio Mario Negri Sud, Santa Maria Imbaro, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Chabielska
authors.forename: V
authors.initials: V
authors.lastname: Kolpakov
authors.forename: M C
authors.initials: MC
authors.lastname: D'Adamo
authors.forename: A
authors.initials: A
authors.lastname: De Curtis
authors.forename: W
authors.initials: W
authors.lastname: Buczko
authors.forename: L
authors.initials: L
authors.lastname: Iacoviello
authors.forename: M B
authors.initials: MB
authors.lastname: Donati
mesh_terms: D015121:6-Ketoprostaglandin F1 alpha / Q000097:blood; D000818:Animals; D001011:Aorta; D001779:Blood Coagulation Factors / Q000378:metabolism; D001807:Blood Vessel Prosthesis; D005337:Fibrin / Q000378:metabolism; D005342:Fibrinolysis; D006487:Hemostasis; D007958:Leukocyte Count; D008297:Male; D010974:Platelet Aggregation; D010976:Platelet Count; D019736:Prostheses and Implants; D051381:Rats; D013927:Thrombosis / Q000473:pathology*; D013997:Time Factors
chemical_list: D001779:Blood Coagulation Factors; D015121:6-Ketoprostaglandin F1 alpha; D005337:Fibrin