title: Protective effect of the combined vaccine prepared from cell-free-antigen of Actinobacillus pleuropneumoniae serotypes 1, 2 and 5 in pigs.
abstract: Cell-free-antigens prepared from a concentrated culture supernatant of Actinobacillus pleuropneumoniae (A. pleuropneumoniae) serotypes 1, 2 and 5 were mixed and emulsified with oil adjuvant. The combined vaccine of these 3 serotypes of A. pleuropneumoniae was tested for its ability to confer protection. Pigs immunized with the combined vaccine survived and showed no clinical signs against an intratracheal challenge with A. pleuropneumoniae. In contrast, control pigs inoculated with concentrated culture media emulsified with oil adjuvant developed typical symptoms of pleuropneumonia after challenge inoculation.
authors.affiliation: Division of Veterinary Microbiology, Kyoto Biken Laboratories, Japan.
authors.forename: E
authors.initials: E
authors.lastname: Oishi
authors.forename: T
authors.initials: T
authors.lastname: Kitajima
authors.forename: Y
authors.initials: Y
authors.lastname: Koyama
authors.forename: T
authors.initials: T
authors.lastname: Ohgitani
authors.forename: S
authors.initials: S
authors.lastname: Katayama
authors.forename: T
authors.initials: T
authors.lastname: Okabe
mesh_terms: D000189:Actinobacillus Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D016977:Actinobacillus pleuropneumoniae / Q000145:classification / Q000276:immunology; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000942:Antigens, Bacterial; D001428:Bacterial Vaccines / Q000302:isolation & purification; D002474:Cell-Free System; D004797:Enzyme-Linked Immunosorbent Assay; D007114:Immunization; D008168:Lung / Q000382:microbiology / Q000473:pathology; D012703:Serotyping; D013552:Swine; D013553:Swine Diseases
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001428:Bacterial Vaccines