Mosaic type of the nontoxic-nonhemaggulutinin component gene in Clostridium botulinum type A strain isolated from infant botulism in Japan.
The gene encoding the nontoxic-nonhemaggulutinin (NTNH) component was amplified by the PCR technique using two primer sets and the DNA template from Clostridium botulinum type A strain 7I03-H isolated from infant botulism in Japan. The nucleotide sequence revealed that the NTNH gene was composed of 1,193 amino acids with a molecular weight of 130868.08. Furthermore, the N-terminal half side and C-terminal half side of the NTNH component were similar to the NTNH component of type C and type A, respectively. These results indicate that the NTNH component gene codes the mosaic NTNH component composed of type A and type C. The hemaggulutinin gene, aha, and ORF-22 gene, orf-22a, were undetectable in the region upstream of the NTNH component gene, ant. Therefore, orf-22a is not thought to play a key role in the expression of botulinum type A progenitor toxin gene.
Kubota
T
T
Department of Microbiology, Sapporo Medical University School of Medicine, Hokkaido, Japan.
Shirakawa
S
S
Kozaki
S
S
Isogai
E
E
Isogai
H
H
Kimura
K
K
Fujii
N
N
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D001905:Botulinum Toxins / Q000633:toxicity*; D001906:Botulism / Q000382:microbiology*; D003001:Cloning, Molecular; D003014:Clostridium botulinum / Q000235:genetics* / Q000302:isolation & purification; D004269:DNA, Bacterial; D006801:Humans; D007231:Infant, Newborn; D008969:Molecular Sequence Data; D009030:Mosaicism; D009498:Neurotoxins / Q000633:toxicity*; D016366:Open Reading Frames; D017386:Sequence Homology, Amino Acid
D001426:Bacterial Proteins; D004269:DNA, Bacterial; C088300:NTNH protein, Clostridium botulinum; D009498:Neurotoxins; D001905:Botulinum Toxins