title: Thrombolysis at a controlled pressure prolongs the survival of skin flaps treated with superoxide dismutase.
abstract: The role of thrombolysis in reestablishing patency in the microcirculation following ischemia, and thereby improving the efficacy of agents attenuating reperfusion injury, such as the oxygen free radical scavenger, superoxide dismutase (SOD), was investigated in a rat model. Abdominal skin flaps were subjected to normothermic ischemia induced by complete occlusion of the pedicle for periods of 12, 13, 14, 16, 18, 20, 22, and 24 hr. In Group 1 (n = 64), all animals received flap washout using 100,000U urokinase (manual injection) followed by 7,500 IU SOD given intra-arterially immediately prior to reperfusion. Animals in Group 2 received flap washout consisting of 100,000U urokinase given via a pressurized delivery system, followed by 7,500 IU SOD. Results demonstrated a statistically significant improvement in flap survival in Group 2. The authors concluded that thrombolytic therapy may be useful in improving the delivery of agents, such as SOD, which attenuate reperfusion injury in skin flaps.
authors.affiliation: Department of Surgery, Mt. Sinai Medical Center, New York, NY 10029, USA.
authors.forename: M B
authors.initials: MB
authors.lastname: Hirigoyen
authors.forename: A
authors.initials: A
authors.lastname: Prabhat
authors.forename: W X
authors.initials: WX
authors.lastname: Zhang
authors.forename: M L
authors.initials: ML
authors.lastname: Urken
authors.forename: H
authors.initials: H
authors.lastname: Weinberg
mesh_terms: D000818:Animals; D005260:Female; D005343:Fibrinolytic Agents / Q000008:administration & dosage*; D016166:Free Radical Scavengers / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000517:prevention & control*; D013482:Superoxide Dismutase / Q000627:therapeutic use*; D013524:Surgical Flaps / Q000502:physiology; D015912:Thrombolytic Therapy / Q000379:methods*; D013997:Time Factors; D014568:Urokinase-Type Plasminogen Activator / Q000008:administration & dosage*
chemical_list: D005343:Fibrinolytic Agents; D016166:Free Radical Scavengers; D013482:Superoxide Dismutase; D014568:Urokinase-Type Plasminogen Activator