An improved method for rapid analysis of the fatty acids of glycerolipids.
An improved rapid procedure to determine the fatty acid composition of glycerolipids is described. The procedure includes KOH-catalyzed transesterification and high-speed gas chromatography. Glycerolipids (20-40 mg) were mixed with 2 mL of hexane and 0.2 mL of 2 M methanolic KOH at room temperature for 1-2 min. The fatty acid methyl esters in the hexane layer were analyzed by gas chromatography on 10% SP-2340 at 240 degrees C. Methyl linolenate and docosahexaenoate eluted within 2 and 5 min, respectively. Analysis was thus completed within 5 min for common vegetable oils and 8 min for fish oils.
Ichihara
K
K
Department of Agricultural Chemistry, Kyoto Prefectural University, Japan.
Shibahara
A
A
Yamamoto
K
K
Nakayama
T
T
D002788:Cholesterol Esters / Q000032:analysis; D002849:Chromatography, Gas / Q000379:methods; D002855:Chromatography, Thin Layer / Q000379:methods; D004952:Esters; D005227:Fatty Acids / Q000032:analysis*; D038983:Galactolipids; D005989:Glycerides / Q000737:chemistry*; D006017:Glycolipids / Q000032:analysis; D006878:Hydroxides; D009829:Oleic Acids / Q000032:analysis; D017680:Potassium Compounds; D012680:Sensitivity and Specificity; D012997:Solvents; D014280:Triglycerides / Q000737:chemistry*
D002788:Cholesterol Esters; D004952:Esters; D005227:Fatty Acids; D038983:Galactolipids; D005989:Glycerides; D006017:Glycolipids; D006878:Hydroxides; D009829:Oleic Acids; D017680:Potassium Compounds; D012997:Solvents; D014280:Triglycerides; C007388:digalactosyldiacylglycerol; C014409:cholesteryl oleate; C066528:oleyl oleate; C029943:potassium hydroxide