Three different states of the chromatin structure of the mouse peripherin gene.
The chromatin structure of the mouse peripherin gene domain was analyzed in peripherin-positive and -negative cell lines. At least nine DNase I hypersensitive sites (HSS) are present within the 20-kb peripherin domain in the mouse neuroblastoma cell lines which express peripherin. Three of them are situated in intron I and intron III, the others being distributed within the 5' flanking region up to -5.5 kb. The presence of these sites was also investigated in the peripherin chromatin domain of peripherin-negative cell lines. Two other types of HSS distribution were observed along the peripherin gene according to the category of cell considered: constantly peripherin-negative cells, or negative cells arising from transiently peripherin-expressing precursors. From comparison of HSS patterns in these cell lines with those of neuroblastoma cells, it can be predicted that HSS located in the region -1500/+800 bp participate in cell-specific expression of the mouse peripherin gene.
Karpov
V
V
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia.
Fauquet
M
M
Lecomte
M J
MJ
Portier
M M
MM
D016475:3T3 Cells; D000818:Animals; D002460:Cell Line; D002843:Chromatin / Q000648:ultrastructure*; D004247:DNA / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D004273:DNA, Neoplasm / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D003850:Deoxyribonuclease I; D005136:Eye Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D007340:Insulinoma; D007381:Intermediate Filament Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D007438:Introns; D008546:Melanoma, Experimental; D008562:Membrane Glycoproteins; D051379:Mice; D009419:Nerve Tissue Proteins; D009447:Neuroblastoma; D009479:Neuropeptides / Q000096:biosynthesis / Q000235:genetics*; D010190:Pancreatic Neoplasms; D064531:Peripherins; D010911:Pituitary Neoplasms; D012045:Regulatory Sequences, Nucleic Acid; D012583:Schwann Cells; D014407:Tumor Cells, Cultured
D002843:Chromatin; D004273:DNA, Neoplasm; D005136:Eye Proteins; D007381:Intermediate Filament Proteins; D008562:Membrane Glycoproteins; D009419:Nerve Tissue Proteins; D009479:Neuropeptides; D064531:Peripherins; D004247:DNA; D003850:Deoxyribonuclease I