An adenovirus encoding CuZnSOD protects cultured striatal neurones against glutamate toxicity.
Superoxide dismutase (SOD), a key enzyme in the detoxification of free radicals, catalyses the dismutation of superoxide O2.- to oxygen and hydrogen peroxide (H2O2). It is therefore a promising candidate for gene transfer therapy of neurological diseases in which free radicals are thought to be involved. We have constructed a recombinant adenoviral vector containing the human copper-zinc SOD cDNA. Using this vector we were able to drive the production of an active human copper-zinc SOD protein (hCuZnSOD) in various cell lines and primary cultures. Infection of striatal cells with a recombinant adenovirus expressing hCuZnSOD protected these cells from glutamate-induced cell death.
Barkats
M
M
Laboratoire mixte Rhône-Poulenc-RORER/CNRS C9923, CERVI, Hôpital de la Pitié Salpêtrière, Paris, France.
Bemelmans
A P
AP
Geoffroy
M C
MC
Robert
J J
JJ
Loquet
I
I
Horellou
P
P
Revah
F
F
Mallet
J
J
D000256:Adenoviridae / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D005822:Genetic Vectors / Q000235:genetics*; D018698:Glutamic Acid / Q000633:toxicity*; D006801:Humans; D007150:Immunohistochemistry; D051379:Mice; D017072:Neostriatum / Q000166:cytology*; D009474:Neurons / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism*
D018698:Glutamic Acid; D013482:Superoxide Dismutase