title: Distribution of GABAA, GABAB, and glycine receptors in the central auditory system of the big brown bat, Eptesicus fuscus.
abstract: Quantitative autoradiographic techniques were used to compare the distribution of GABAA, GABAB, and glycine receptors in the subcortical auditory pathway of the big brown bat, Eptesicus fuscus. For GABAA receptors, the ligand used was 35S-t-butylbicyclophosphorothionate (TBPS) for GABAB receptors, 3H-GABA was used as a ligand in the presence of isoguvacine to block binding to GABAA sites; for glycine, the ligand used was 3H-strychnine. In the subcortical auditory nuclei there appears to be at least a partial complementarity in the distribution of GABAA receptors labeled with 35S-TBPS and glycine receptors labeled with 3H-strychnine, GABAA receptors were concentrated mainly in the inferior colliculus (IC) and medial geniculate nucleus, whereas glycine receptors were concentrated mainly in nuclei below the level of the IC. Within the IC, there was a graded spatial distribution of 35S-TBPS binding; the most dense labeling was in the dorsomedial region, but very sparse labeling was observed in the ventrolateral region. There was also a graded spatial distribution of 3H-strychnine binding. The most dense labeling was in the ventral and lateral regions and the weakest labeling was in the dorsomedial region. Thus, in the IC, the distribution of 35S-TBPS was complementary to that of 3H-strychnine. GABAB receptors were distributed at a low level throughout the subcortical auditory nuclei, but were most prominent in the dorsomedial part of the IC.
authors.affiliation: Department of Neurobiology, Duke University Medical Center, Durham, North Carolina 27710, USA.
authors.forename: B M
authors.initials: BM
authors.lastname: Fubara
authors.forename: J H
authors.initials: JH
authors.lastname: Casseday
authors.forename: E
authors.initials: E
authors.lastname: Covey
authors.forename: R D
authors.initials: RD
authors.lastname: Schwartz-Bloom
mesh_terms: D000818:Animals; D001306:Auditory Pathways / Q000737:chemistry*; D001933:Brain Stem / Q000737:chemistry*; D002685:Chiroptera / Q000378:metabolism*; D007245:Inferior Colliculi / Q000737:chemistry; D009433:Neural Inhibition / Q000502:physiology; D011963:Receptors, GABA-A / Q000032:analysis*; D018080:Receptors, GABA-B / Q000032:analysis*; D018009:Receptors, Glycine / Q000032:analysis*
chemical_list: D011963:Receptors, GABA-A; D018080:Receptors, GABA-B; D018009:Receptors, Glycine