title: Effect of interleukin-2 and interleukin-4 on lymphocytes from peribronchial lymphatic tissue.
abstract: Lymphocyte activation in spleen and peribronchial lymphatic tissue (PBLT) following stimulation with T-cell mitogens and lymphokines was investigated in the hamster. Optimal mitogen-induced cell proliferation was achieved after culturing for 6 days in vitro. Interleukin-2 (IL-2) and interleukin-4 (IL-4) did not induce DNA synthesis in resting T-cells from either spleen or PBLT.IL-2 or IL-4 in combination with concanavalin A promoted splenic T-cell proliferation. In contrast, in PBLT, IL-2-but not IL-4-enhanced cell proliferation (p < .001). The findings indicate that PBLT represents an independent compartment of the immune system. Furthermore, in the hamster PBLT cells consist predominantly of IL-2-responsive cells, i.e., are of the Th1 type. Immunological pathogenesis of lung injury can therefore be studied by functional analysis of PBLT lymphocytes.
authors.affiliation: Department of Bone Marrow Transplantation, Mallory Institute of Pathology, Boston, MA 02118, USA.
authors.forename: R
authors.initials: R
authors.lastname: Or
authors.forename: I S
authors.initials: IS
authors.lastname: Lossos
authors.forename: E
authors.initials: E
authors.lastname: Hirschfeld
authors.forename: R
authors.initials: R
authors.lastname: Breuer
mesh_terms: D000818:Animals; D001980:Bronchi / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D002455:Cell Division / Q000187:drug effects; D006224:Cricetinae; D004247:DNA / Q000096:biosynthesis* / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D007376:Interleukin-2 / Q000008:administration & dosage*; D015847:Interleukin-4 / Q000008:administration & dosage*; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008934:Mitogens / Q000008:administration & dosage*
chemical_list: D007376:Interleukin-2; D008934:Mitogens; D015847:Interleukin-4; D004247:DNA