Induction and repair of DNA damage in UV-irradiated human lymphocytes. Spectral differences and repair kinetics.
The alkaline elution assay has been employed to study the induction and repair kinetics of DNA damage in human lymphocytes after irradiation with biologically relevant doses of UVB (297 and 302 nm) or UVA (365 nm) radiation. At 365 nm, when the predominant lesions are single-strand breaks, the rate of lesion induction was 1.5 x 10(-3) per 10(8) Da per kJ m-2. The number of breaks decayed with a half-life of about 50 min after a dose of 20 kJ m-2. In the UVB region, cyclobutyl pyrimidine dimers and 6-4 photoproducts are formed, both of which are repairable via the nucleotide excision repair pathway. By using repair inhibitors, the rate of induction of such lesions at 297 and 302 nm was found to be 0.07 per 10(8) Da per J m-2. Lesions were removed with a half-life of about 100 min. Mathematical modelling of the excision repair process revealed a time-dependent polymerization-ligation rate: after an initial lag phase the polymerization-ligation rate increased, reaching 50% of its maximum rate at 80-100 min after the start of repair incubation. This course of development might be due to a damage-associated regulation of DNA precursors synthesis.
Henriksen
E K
EK
Institute for Cancer Research, Norwegian Radium Hospital, Oslo, Norway.
Moan
J
J
Kaalhus
O
O
Brunborg
G
G
D002470:Cell Survival / Q000528:radiation effects; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis / Q000528:radiation effects; D004249:DNA Damage; D004260:DNA Repair; D004307:Dose-Response Relationship, Radiation; D006801:Humans; D007700:Kinetics; D008214:Lymphocytes / Q000166:cytology / Q000502:physiology / Q000528:radiation effects*; D013936:Thymidine / Q000378:metabolism; D013997:Time Factors; D014466:Ultraviolet Rays
D004247:DNA; D013936:Thymidine