Effect of vaccination of ponies with A4 anti-idiotypic antibody on serum idiotype (1C9) and antilipid A concentration.
OBJECTIVE
To evaluate the humoral response of horses to vaccination, using a murine monoclonal anti-idiotypic antibody (A4) that shares an epitope with lipid A.


METHODS
Serum concentrations of antilipid A antibody and 1C9 (epitope on murine monoclonal antilipid A antibody) were measured serially during the period of vaccination with A4.


METHODS
6 clinically normal adult ponies.


METHODS
Ponies were inoculated IM 3 times at monthly intervals with A4. Two weeks after each inoculation, serum was obtained and was assayed by ELISA for antilipid A and 1C9 concentrations. Additional vaccinations were given to 2 of the ponies after a several-month rest period.


RESULTS
There was significant increase in 1C9 concentration (P < 0.0001) during the period of vaccination and a trend toward increased antilipid A concentration. The latter effect was not significant (P = 0.055). Additional vaccinations produced further increase in serum 1C9 concentration; antilipid A concentration increased in one of these ponies but not the other. Maximal antilipid A concentration recorded in these ponies was approximately 6 times preimmune concentration and was comparable to that found in a commercial antiendotoxin antiserum. 1C9 also was detected in the commercial antiserum.


CONCLUSIONS
A4 anti-idiotype vaccination of horses is safe and may be effective in eliciting an antibody response against endotoxin. The finding of 1C9 in a commercial antiendotoxin antiserum indicates that this idiotype may be part of the normal polyclonal antibody response of horses to endotoxin.


CONCLUSIONS
It may be possible to use anti-idiotypic antibody vaccination in horses to induce protection against the effects of endotoxin.
BonenClark
G D
GD
Department of Large Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, Gainesville 32610, USA.
MacKay
R J
RJ
Ward
R E
RE
Sheerin
B
B
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000097:blood* / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000097:blood* / Q000276:immunology; D004731:Endotoxins / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D000939:Epitopes / Q000276:immunology; D006736:Horses / Q000097:blood / Q000276:immunology*; D007106:Immune Sera / Q000276:immunology; D008050:Lipid A / Q000276:immunology*; D008297:Male; D051379:Mice; D014612:Vaccines / Q000276:immunology / Q000494:pharmacology*
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D004731:Endotoxins; D000939:Epitopes; D007106:Immune Sera; D008050:Lipid A; D014612:Vaccines