Modulation of WC1, a lineage-specific cell surface molecule of gamma/delta T cells augments cellular proliferation.
WC1, also known as T19, is the only unique gamma/delta T-cell differentiation antigen described to date other than the gamma/delta T-cell receptor. We present evidence that modulation of WC1 results in augmented proliferation of gamma/delta T cells. Immobilized IL-A29, a monoclonal antibody (mAb) specific for WC1, augmented proliferation of gamma/delta T cells in the autologous mixed leucocyte reaction (AMLR) as well as proliferation induced by either anti-CD3 or anti-CD5 mAb. In contrast, anti-CD5 mAb did not increase proliferation in the AMLR even though both CD5 and WC1 are members of the scavenger receptor cysteine-rich family of proteins and are expressed by bovine peripheral blood gamma/delta T cells. IL-A29 did not induce proliferation when assessed alone or in the presence of either phorbol myristate acetate (PMA) or interleukin-2. IL-A29 also did not induce detectable calcium mobilization when evaluated in the presence of monocytes, PMA, or following cross-linking of IL-A29 with anti-immunoglobulin antibody. We conclude that WC1 is a gamma/delta T-cell lineage-specific cell-surface differentiation antigen which is involved in activation of gamma/delta T cells using an as yet unidentified pathway.
Hanby-Flarida
M D
MD
Department of Microbiology and Comprehensive Cancer Center, Ohio State University, Columbus, USA.
Trask
O J
OJ
Yang
T J
TJ
Baldwin
C L
CL
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology*; D000954:Antigens, Surface / Q000276:immunology*; D002118:Calcium / Q000378:metabolism; D002417:Cattle; D002455:Cell Division / Q000276:immunology; D005260:Female; D005434:Flow Cytometry; D007376:Interleukin-2 / Q000276:immunology; D008562:Membrane Glycoproteins / Q000276:immunology*; D016692:Receptors, Antigen, T-Cell, gamma-delta; D015398:Signal Transduction / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism
D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface; C086500:DMBT1 protein, Bos taurus; D007376:Interleukin-2; D008562:Membrane Glycoproteins; D016692:Receptors, Antigen, T-Cell, gamma-delta; D002118:Calcium