title: The action of phenazine methosulphate in causing cellular damage in the isolated mouse soleus muscle preparation.
abstract: Exposure of the isolated mouse soleus preparation to 1.0 mM phenazine methosulphate (PMS) caused (i) a slow and modest release of creatine kinase (CK) that was exacerbated by removal of extracellular Ca2+, (ii) a specific type of ultrastructural damage, namely a characteristic spacing of the myofibrils, (iii) swelling of the mitochondria, indicating a modest rise in [Ca2+]i, and (iv) swelling of the sarcoplasmic reticulum (SR). It is suggested that PMS (i) activates a sarcolemma oxidoreductase which synergistically interacts with raised [Ca2+]i to cause modest CK efflux and (ii) activates an oxidoreductase on the SR, thereby generating electrons which directly modify the integrity and organisation of the contractile apparatus.
authors.affiliation: Department of Zoology, School of Life Sciences, University of Liverpool, UK.
authors.forename: K E
authors.initials: KE
authors.lastname: McCall
authors.forename: C J
authors.initials: CJ
authors.lastname: Duncan
mesh_terms: D000818:Animals; D003402:Creatine Kinase / Q000187:drug effects / Q000378:metabolism; D066298:In Vitro Techniques; D008773:Methylphenazonium Methosulfate / Q000633:toxicity*; D051379:Mice; D008807:Mice, Inbred BALB C; D018482:Muscle, Skeletal / Q000187:drug effects* / Q000201:enzymology / Q000648:ultrastructure; D009336:Necrosis
chemical_list: D008773:Methylphenazonium Methosulfate; D003402:Creatine Kinase