[Expression of the extracellular domain (1-341) of rat ovarian lutropin receptor in insect cells and preliminary characterization of the expressed product].
Extracellular domain residues 1-341 (designed R 341) of luteinizing hormone/human chorionic gonadotropin receptor (LH/hCG receptor) has high binding affinity for ligand. This paper describes expression of cDNA coding for R 341 in insect cells and preliminary identification of the expressed protein. SDS-PAGE silver staining and immunoblotting analysis show expressed product appears in two bands, major band has molecular weight 38.5 Kd, and weak band, 40.0 Kd. Ligand binding immunoblotting and 125I-hCG-binding blotting analysis indicate that expressed product R 341 has specific binding affinity for ligand. Ligand binding assay and Scatchard analysis indicate that recombinant receptor R 341 has high binding affinity for hCG and Kd is 5.68 x 10(-10) mol/L.
Shen
Q X
QX
Shanghai Institute of Cell Biology, Academia Sinica.
Li
C L
CL
Shen
H
H
Liu
H H
HH
Huang
Y G
YG
Chen
W Y
WY
Bahl
O P
OP
D000818:Animals; D016367:Baculoviridae / Q000235:genetics*; D001483:Base Sequence; D002478:Cells, Cultured; D018076:DNA, Complementary; D005260:Female; D015870:Gene Expression; D007313:Insecta / Q000166:cytology / Q000378:metabolism*; D008969:Molecular Sequence Data; D010053:Ovary / Q000378:metabolism; D010446:Peptide Fragments / Q000096:biosynthesis*; D011869:Radioligand Assay; D051381:Rats; D011966:Receptors, LHRH / Q000096:biosynthesis* / Q000235:genetics; D014162:Transfection
D018076:DNA, Complementary; D010446:Peptide Fragments; D011966:Receptors, LHRH