Basic fibroblast growth factor within endothelial cells during vascularization of the anterior pituitary.
BACKGROUND
Basic fibroblast growth factor (FGF-2), a potent angiogenic peptide, is known to be present in gonadotropes of the anterior pituitary parenchyma of rats and mice, and has been isolated from endothelial cells of many organs. Its localization within endothelial cells has not been determined, nor the mechanisms by which it might be released from endothelial cells during normal organogenesis.


METHODS
Localization of FGF within endothelial cells of the anterior pituitary was accomplished by immunocytochemistry and studied by light- and electron microscopy. Capillaries within the anterior pituitary were studied in fetal rats from day 15 to term, and in adult rats.


RESULTS
At the onset stages of vascularization (15-18 days fetal), the cytoplasm of the endothelial cells of many of the invading, immature capillaries (thick-walled with few or no fenestrations) was intensely immunopositive for FGF. Immunoprecipitate-filled blebs and slender cytoplasmic processes projected from the endothelial cells into the presumptive pericapillary space and toward the parenchymal cells. As gestation progressed (19-20 day fetal), and an increasing number of capillaries acquired the features characteristic of capillaries in the anterior pituitary of adult animals, i.e., thin-walled and fenestrated, there were fewer capillaries demonstrating immunopositivity for FGF. Foci of released FGF, i.e., extracellular, were occasionally evident within the presumptive pericapillary spaces throughout gestation. By comparison, capillaries of the anterior pituitary of adult rats did not contain immunostainable FGF in their cytoplasm, nor were any blebs and/or processes filled with immunoprecipitate evident. However capillaries did reveal an immunopositive enhancement of their lumenal and ablumenal surfaces.


CONCLUSIONS
During vascularization of the anterior pituitary, FGF within the cytoplasm of endothelial cells is released from blebs and/or processes of endothelial cells, and after the capillary bed is stabilized postnatally, these characteristics of vascularization are absent.
Schechter
J
J
Department of Cell and Neurobiology, University of Southern California School of Medicine, Los Angeles, USA.
Pattison
A
A
Pattison
T
T
D000818:Animals; D002196:Capillaries / Q000378:metabolism / Q000648:ultrastructure; D003593:Cytoplasm / Q000648:ultrastructure; D004730:Endothelium, Vascular / Q000737:chemistry* / Q000196:embryology / Q000648:ultrastructure; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000032:analysis* / Q000096:biosynthesis; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D018919:Neovascularization, Physiologic / Q000502:physiology*; D010902:Pituitary Gland / Q000098:blood supply* / Q000737:chemistry / Q000196:embryology; D011247:Pregnancy; D051381:Rats; D011916:Rats, Inbred F344; D017207:Rats, Sprague-Dawley
D016222:Fibroblast Growth Factor 2