title: Polymorphism in Brucella strains detected by studying distribution of two short repetitive DNA elements.
abstract: Thirty-four Brucella reference or field strains representing all the species and biovars were studied by repetitive element sequence-based PCR, a PCR using primers complementary to two enterobacterial short repetitive sequences: repetitive extragenic palindromic and enterobacterial repetitive intergenic consensus sequences. All the stains showed a positive amplification, suggesting that the Brucella genome contains such sequences. Repetitive extragenic palindromic PCR was less discriminating than enterobacterial repetitive intergenic consensus PCR in terms of distinguishing strains, but a combination of the two methods was able to distinguish all the isolates, except for some strains belonging to biovars 3 and 9 of Brucella abortus. Repetitive element sequence-based PCR appears to be a simple and useful method for the study of brucellosis epidemiology.
authors.affiliation: Institut National de la Santé et de la Recherche Médicale, Unité 431, Faculté de Médecine, Nîmes, France.
authors.forename: E
authors.initials: E
authors.lastname: Mercier
authors.forename: E
authors.initials: E
authors.lastname: Jumas-Bilak
authors.forename: A
authors.initials: A
authors.lastname: Allardet-Servent
authors.forename: D
authors.initials: D
authors.lastname: O'Callaghan
authors.forename: M
authors.initials: M
authors.lastname: Ramuz
mesh_terms: D001483:Base Sequence; D002002:Brucella / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D002006:Brucellosis / Q000453:epidemiology / Q000382:microbiology; D016172:DNA Fingerprinting; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012091:Repetitive Sequences, Nucleic Acid
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial