title: In vitro biosynthesis of androgens in the Australian lungfish, Neoceratodus forsteri.
abstract: The serum concentration of testosterone was estimated from a population of wild lungfish over 6-7 years of sampling. Male lungfish were found to have high circulating levels of testosterone (approximately 50 ng/ml) which varied seasonally and could be correlated with spermatogenesis as judged by testis histology. Incubation of testis tissue slices with [3H]progesterone, [3H]17-hydroxyprogesterone, or [3H]testosterone confirmed that testosterone is the major androgen in Neoceratodus. Not even trace amounts of 11-keto- or 11 beta-hydroxytestosterone or 5 alpha-dihydrotestosterone could be identified by TLC separations. There was little or no conjugation of steroids by the testes, except during the spawning season, when glucuronides of androstenedione and testosterone were produced.
authors.affiliation: School of Biological Sciences, Macquarie University, Sydney, Australia.
authors.forename: J M
authors.initials: JM
authors.lastname: Joss
authors.forename: A
authors.initials: A
authors.lastname: Edwards
authors.forename: D E
authors.initials: DE
authors.lastname: Kime
mesh_terms: D000728:Androgens / Q000032:analysis / Q000096:biosynthesis*; D000818:Animals; D002855:Chromatography, Thin Layer; D005399:Fishes / Q000502:physiology*; D008297:Male; D011863:Radioimmunoassay; D013056:Spectrophotometry, Ultraviolet; D013091:Spermatogenesis / Q000187:drug effects; D013737:Testis / Q000033:anatomy & histology / Q000254:growth & development / Q000378:metabolism*; D013739:Testosterone / Q000097:blood
chemical_list: D000728:Androgens; D013739:Testosterone