[Effect of HC-3 on electroacupuncture-induced immunoregulation].
Our previous study suggested that peripheral sympathetic nerve played an important role in the electroacupuncture (EA)-induced immunomodulation. The aim of this study is to explore the role of peripheral parasympathetic nerve in it. All mice are preimmunized with SRBC (sheep red blood cell) 4 days before experiment. The animals in different groups were injected i.p. with Hemicholine-3 (HC-3, an ACh synthesis blocker) or physiological saline 3 hr before EA. The main results are as follows: 1. In saline group, the LTT (lymphocyte transformation test), IL-2 (interleukin-2) of spleen and lymphocyte number of thymus are markedly increased, and the other immune parameters have no change after EA. 2. In HC-3 control group, the LTT, IL-2 are decreased significantly and the other immune parameters have not shown influence. 3. In the HC-3 with EA group, the magnitude of LTT, IL-2 did not significantly change as compared with HC-3 alone, it is especially noteworthy that the other unchanged parameters by HC-3 such as spleen weight, spleen index, thymus weight, thymus index, number of spleen cells, spleen LZM(lysozyme) and thymus LZM are clearly decreased after EA. The above results suggest that peripheral parasympathetic nerve may play some promoting effect in the EA-induced immunomodulation. Its effect may be mediated by ACh released from parasympathetic nerve endings.
Zhao
X
X
Shanxi Academy of Traditional Chinese Medicine, Xian.
D000276:Adjuvants, Immunologic / Q000494:pharmacology; D000818:Animals; D018680:Cholinergic Antagonists / Q000494:pharmacology*; D015671:Electroacupuncture; D005260:Female; D006426:Hemicholinium 3 / Q000494:pharmacology*; D007376:Interleukin-2 / Q000276:immunology; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D013601:T-Lymphocytes / Q000276:immunology*; D013950:Thymus Gland / Q000166:cytology
D000276:Adjuvants, Immunologic; D018680:Cholinergic Antagonists; D007376:Interleukin-2; D006426:Hemicholinium 3