Differential effects of progestins and estrogens on long control regions of human papillomavirus types 16 and 18.
We have studied effects of a series of progestins and estrogens on regulation of human papillomavirus (HPV) types 16 and 18 gene expression via long control region (LCR), using a transient expression assay which represented the episomal state of HPVs in precancerous lesions. Results showed that HPV gene expression induced by these steroid analogs were diverse. Gene expression via HPV16-LCR was upregulated 2-3 fold under treatment with 10(-7) M estriol, 17 beta-estradiol, and several progestins including pregnenolone, 17 alpha-hydroxy-progesterone, norethynodrel and cyproterone acetate. However, transcription via HPV18-LCR was not or weakly stimulated by these analogs. Our results can divide these analogs into HPV-enhancing and HPV-nonenhancing groups and accordingly impact the relative risk of individual oral contraceptive ingredient in cervical carcinogenesis.
Chen
Y H
YH
Department of Pharmacy, School of Medicine, National Taiwan University, Taipei, Taiwan.
Huang
L H
LH
Chen
T M
TM
D001483:Base Sequence; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D004967:Estrogens / Q000378:metabolism / Q000494:pharmacology*; D005260:Female; D015967:Gene Expression Regulation, Viral / Q000187:drug effects*; D017930:Genes, Reporter; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D009856:Oncogene Proteins, Viral / Q000235:genetics; D027383:Papillomaviridae / Q000235:genetics* / Q000302:isolation & purification; D010957:Plasmids; D011230:Precancerous Conditions / Q000821:virology; D011372:Progestins / Q000378:metabolism / Q000494:pharmacology*; D011485:Protein Binding; D012045:Regulatory Sequences, Nucleic Acid; D013329:Structure-Activity Relationship; D002583:Uterine Cervical Neoplasms / Q000821:virology
D004967:Estrogens; D009856:Oncogene Proteins, Viral; D011372:Progestins; D015500:Chloramphenicol O-Acetyltransferase