title: Correlation between poliovirus type 1 Mahoney replication in blood cells and neurovirulence.
abstract: Poliovirus (PV) is not often described as a monocyte- or macrophage-tropic virus; however, previous work indicated that neurovirulent PV type 1 Mahoney [PV(1)Mahoney] can productively infect primary human monocytes. To determine whether this replication has a functional role in pathogenesis, primary human mononuclear blood cells were infected with pairs of attenuated and neurovirulent strains of PV. Two neurovirulent strains of PV, PV(1)Mahoney and PV(2)MEF-1, replicated faster and to higher titers than attenuated counterparts PV(1)Sabin and PV(2)W-2, respectively, in primary human monocytes, suggesting that this replication may contribute to pathogenesis. PV(3)Leon grew weakly, while PV(3)Sabin, PV(2)Sabin, and PV(2) P712 did not replicate in these cells, perhaps because of their slow replication cycle. In U937 cells, a monocytelike cell line, PV(1)Mahoney replicated but PV(1)Sabin did not, while both grew well in HeLa cells. When molecular recombinants of PV(1)Mahoney and PV(1)Sabin were assessed, a correlation between neurovirulence and the ability to replicate in primary human mononuclear blood cells was found. Surprisingly, infectious centers assays with primary human mononuclear blood cells and U937 cells indicated that despite the lower overall viral yield, more cells are initially infected with the attenuated viruses. These results indicate that there are virulence-specific differences in the ability of PV(1)Mahoney to replicate in monocytes and suggest that there may be factors in monocytes that virulent strains of PV require.
authors.affiliation: Department of Microbiology, Immunology, and Parasitology, Louisiana State University Medical Center, New Orleans 70112, USA. mfreis@lsumc.edu
authors.forename: M S
authors.initials: MS
authors.lastname: Freistadt
authors.forename: K E
authors.initials: KE
authors.lastname: Eberle
mesh_terms: D002460:Cell Line; D006367:HeLa Cells; D006801:Humans; D007700:Kinetics; D007963:Leukocytes, Mononuclear / Q000821:virology*; D009000:Monocytes / Q000821:virology*; D017955:Poliovirus / Q000472:pathogenicity* / Q000502:physiology*; D013045:Species Specificity; D014774:Virulence; D014779:Virus Replication