Polymorphism in Brucella strains detected by studying distribution of two short repetitive DNA elements.
Thirty-four Brucella reference or field strains representing all the species and biovars were studied by repetitive element sequence-based PCR, a PCR using primers complementary to two enterobacterial short repetitive sequences: repetitive extragenic palindromic and enterobacterial repetitive intergenic consensus sequences. All the stains showed a positive amplification, suggesting that the Brucella genome contains such sequences. Repetitive extragenic palindromic PCR was less discriminating than enterobacterial repetitive intergenic consensus PCR in terms of distinguishing strains, but a combination of the two methods was able to distinguish all the isolates, except for some strains belonging to biovars 3 and 9 of Brucella abortus. Repetitive element sequence-based PCR appears to be a simple and useful method for the study of brucellosis epidemiology.
Mercier
E
E
Institut National de la Santé et de la Recherche Médicale, Unité 431, Faculté de Médecine, Nîmes, France.
Jumas-Bilak
E
E
Allardet-Servent
A
A
O'Callaghan
D
D
Ramuz
M
M
D001483:Base Sequence; D002002:Brucella / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D002006:Brucellosis / Q000453:epidemiology / Q000382:microbiology; D016172:DNA Fingerprinting; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012091:Repetitive Sequences, Nucleic Acid
D017931:DNA Primers; D004269:DNA, Bacterial