Expression of mRNA encoding neurotrophic factors and its regulation in a hybrid neuronal cell line.
In a cultured hybrid neuronal cell line (BIM) which was produced between human neuroblastoma cells (IMR32) and thymidine auxotrophs (B3T) of rat nerve-like cells (B103), the mRNAs encoding ciliary neurotrophic factor (CNTF) and neurotrophins were detected by the polymerase chain reaction method. The conditioned medium of BIM cells enhanced choline acetyltransferase (ChAT) activity in septal neurons and survival of ciliary ganglion neurons. The mRNA expression of CNTF and neurotrophins in BIM cells was differently regulated by the stimulation with cAMP, FGF and retinoic acid. These data suggest multiple regulation and collaboration of neurotrophic factors.
Nakanishi
T
T
Department of Biochemistry, Okayama University Dental School, Japan.
Ishii
K
K
Fukushima
N
N
Asanuma
M
M
Iwata
E
E
Ogawa
N
N
D000818:Animals; D001483:Base Sequence; D019208:Brain-Derived Neurotrophic Factor; D002460:Cell Line; D002642:Chick Embryo; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D020934:Ciliary Neurotrophic Factor; D000242:Cyclic AMP / Q000494:pharmacology; D005346:Fibroblast Growth Factors / Q000494:pharmacology; D006801:Humans; D006822:Hybrid Cells; D008969:Molecular Sequence Data; D009414:Nerve Growth Factors / Q000096:biosynthesis* / Q000235:genetics; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000235:genetics; D009447:Neuroblastoma; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D014212:Tretinoin / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D019208:Brain-Derived Neurotrophic Factor; D020934:Ciliary Neurotrophic Factor; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D014212:Tretinoin; D005346:Fibroblast Growth Factors; D000242:Cyclic AMP; D002795:Choline O-Acetyltransferase