[Importance of the leukotriene C4 liberation test for the diagnosis of drug allergy (preliminary results)].
Pharmacological or immunological activation of human basophil induces the expression of membrane markers such as CD63 and the release of histamine and leukotriene C4 (LTC4). LTC4 release test has the advantages of a cellular test which is obviously closer to the physio-pathological reaction than the direct determination of circulating antibodies. Moreover, the determination of a newly formed mediator eliminates the spontaneous release of mediator by simple cellular damage. We present here, after having controlled the reliability of the method for aero-allergens, preliminary results of LTC4 release obtained for haptens (ampicillin, methylprednisolone and acetylsalicylic acid) which are in favour of the use of this method in the complex field of drug allergy diagnosis.
Sainte-Laudy
J
J
Unité d'Immuno-allergologie, laboratoire Cerba, Val d'Oise.
Vallon
C
C
Guérin
J C
JC
D000328:Adult; D000485:Allergens / Q000009:adverse effects* / Q000276:immunology / Q000494:pharmacology; D000667:Ampicillin / Q000009:adverse effects* / Q000276:immunology / Q000494:pharmacology; D000818:Animals; D001241:Aspirin / Q000009:adverse effects* / Q000276:immunology / Q000494:pharmacology; D015549:Basophil Degranulation Test; D001491:Basophils / Q000187:drug effects / Q000378:metabolism*; D002415:Cats; D003875:Drug Eruptions / Q000097:blood / Q000175:diagnosis / Q000209:etiology; D004342:Drug Hypersensitivity / Q000097:blood / Q000175:diagnosis* / Q000209:etiology; D005260:Female; D006241:Haptens / Q000009:adverse effects* / Q000276:immunology / Q000494:pharmacology; D006801:Humans; D017997:Leukotriene C4 / Q000097:blood* / Q000378:metabolism; D008297:Male; D008775:Methylprednisolone / Q000009:adverse effects* / Q000276:immunology / Q000494:pharmacology; D008875:Middle Aged; D012130:Respiratory Hypersensitivity / Q000175:diagnosis / Q000209:etiology; D012882:Skin Tests; D013447:Sulfites / Q000009:adverse effects; D014581:Urticaria / Q000097:blood / Q000139:chemically induced / Q000175:diagnosis
D000485:Allergens; D006241:Haptens; D013447:Sulfites; D017997:Leukotriene C4; D000667:Ampicillin; D001241:Aspirin; D008775:Methylprednisolone
Intérêt du test de libération du leucotriène C4 pour le diagnostic d'une allergie médicamenteuse (résultats préliminaires).