title: In situ 19F MRS measurement of RIF-1 tumor blood volume: corroboration by radioisotope-labeled [125I]-albumin and correlation to tumor size.
abstract: Tumor blood volume (TBV) is an important factor in the metabolism of a tumor and in its response to therapy. Until recently, the only methods to determine TBV were highly invasive and many involved radioisotopes. In this study, a perfluorocarbon (PFC) emulsion, Oxypherol, was monitored by 19F magnetic resonance spectroscopy (MRS). TBVs as determined by 19F MRS of in situ and excised radiation-induced fibrosarcoma (RIF-1) tumors (n = 9), were strongly correlated with the TBV measured by a radioisotope labeled albumin method (slopes of 1.1 and 0.8 with R = 0.86 and 0.91, respectively, by linear regression). In general, the TBV as calculated from the in situ MRS measurements (n = 24) decreased from 28 to 5 ml/100 g tumor mass for tumors ranging in mass from 0.15 to 2 g. However, there was an indication of an initial increase of TBV in tumors smaller than 0.5 g.
authors.affiliation: Magnetic Resonance Research Center, Cleveland Clinic Foundation, OH 44195, USA.
authors.forename: N J
authors.initials: NJ
authors.lastname: Baldwin
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
authors.forename: T C
authors.initials: TC
authors.lastname: Ng
mesh_terms: D000418:Albumins; D000818:Animals; D001802:Blood Substitutes; D001810:Blood Volume; D002855:Chromatography, Thin Layer; D005354:Fibrosarcoma / Q000000981:diagnostic imaging / Q000473:pathology / Q000503:physiopathology*; D005461:Fluorine; D005466:Fluorocarbons; D007457:Iodine Radioisotopes; D016014:Linear Models; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008809:Mice, Inbred C3H; D008815:Mice, Inbred Strains; D009368:Neoplasm Transplantation; D009381:Neoplasms, Radiation-Induced / Q000000981:diagnostic imaging / Q000473:pathology / Q000503:physiopathology*; D011877:Radionuclide Imaging; D012878:Skin Neoplasms / Q000000981:diagnostic imaging / Q000473:pathology / Q000503:physiopathology
chemical_list: D000418:Albumins; D001802:Blood Substitutes; D005466:Fluorocarbons; D007457:Iodine Radioisotopes; D005461:Fluorine; C008807:perfluorotributylamine