[Dose-dependent character of the effect of hydrocortisone on energy metabolism of rat thymocytes].
The influence of the hydrocortisone (GC) different doses on rat's thymocytes energetic metabolism was investigated. It has been demonstrated that as the prolonged action of GC in vivo (20 micrograms/kg of mass during 6 d. daily) as the preincubation of thymocytes with 10 microM GC in vitro are cytotoxic, leading to absolute uncoupling of oxidative phosphorylation (OP) and decreasing the rate of DNP-stimulated respiration speed. The injection of GC (20 micrograms/kg mass) at once before 72 hours to killing resulted the OP uncoupling almost on 2,5 time. Short-time action of GC at the same dose before 3 hours to drilling and so the thymocytes preincubation with 1 microM GC stimulated the transport processes on the level of substrate oxidation without the OP uncoupling. Short-time action of GC in vivo caused the inhibition of respiration, if we used glucose as the oxidative substrate, and stimulated the respiration in a presence of pyruvate Na in incubation media.
Gizatullina
Z Z
ZZ
Sukocheva
O A
OA
Gagel'gans
A I
AI
D000818:Animals; D001692:Biological Transport; D004305:Dose-Response Relationship, Drug; D004734:Energy Metabolism / Q000187:drug effects*; D006854:Hydrocortisone / Q000008:administration & dosage / Q000494:pharmacology*; D066298:In Vitro Techniques; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D010085:Oxidative Phosphorylation; D010100:Oxygen / Q000378:metabolism; D051381:Rats
D010100:Oxygen; D006854:Hydrocortisone
Dozozavisimyĭ kharakter vliianiia gidrokortizona na énergeticheskiĭ metabolizm timotsitov krys.