[Construction of genomic library of L. interrogans serovar lai and preliminary study of recombinant plasmid pDC38].
A genomic library, consisting of approximate 12 000 recombinants, has been constructed for the Leptospira interrogans serovar lai in E. coli. using pUC18 as the vector. Hybridization analysis with the DNA fragment containing OmpL1 gene was performed and 10 positive clones were screened from the genomic library. One of the positive clones, designated pDC38, showed hybridization signal with the DNA of 7 serovars 8 strains of pathogenic leptospires, but not with the DNA of nonpathogenic leptospires (L. biflexa), Leptonama illini, E. coli., and 2 strains of L. interrongans.
Chen
Z
Z
Department of Pathophysiology, Sichuan University.
Dai
B
B
Zhang
Y
Y
Li
S
S
D004274:DNA, Recombinant; D016680:Genome, Bacterial; D015698:Genomic Library; D007921:Leptospira interrogans / Q000235:genetics*; D010957:Plasmids
D004274:DNA, Recombinant