cis-regulatory elements responsible for quantitative regulation of the rice seed storage protein glutelin GluA-3 gene.
The 897 bp 5' flanking region of the rice storage protein glutelin gene (GluA-3) was analyzed to define cis-regulatory elements responsible for the seed specific expression by progressive 5' deletions and gain-of-function experiments using a truncated CaMV 35S promoter (-90/+9). These constructs were transcriptionally fused either to the firefly luciferase (LUX) or beta-glucuronidase (GUS) reporter gene and introduced into tobacco. It was shown that a 121 bp sequence between -437 and -317 from the transcriptional start site is important for the quantitative regulation of the GluA-3 gene expression. This region contained at least two functionally different cis-regulatory elements. One acts as a general positive element and the other is involved in a specific negative regulation in non-seed tissues.
Yoshihara
T
T
Department of Cell Biology, National Institute of Agrobiological Resources, Tsukuba, Japan.
Takaiwa
F
F
D000818:Animals; D001483:Base Sequence; D001517:Coleoptera; D004742:Enhancer Elements, Genetic; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D005966:Glucuronidase / Q000096:biosynthesis; D005983:Glutens / Q000096:biosynthesis* / Q000235:genetics; D008156:Luciferases / Q000096:biosynthesis; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified; D010947:Plants, Toxic; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D012045:Regulatory Sequences, Nucleic Acid; D017384:Sequence Deletion; D012689:Sequence Homology, Nucleic Acid; D014026:Nicotiana / Q000378:metabolism; D014158:Transcription, Genetic
D011993:Recombinant Fusion Proteins; D005983:Glutens; D008156:Luciferases; D005966:Glucuronidase