Inhibition of tumor necrosis factor attenuates physiological sleep in rabbits.
The involvement of tumor necrosis factor (TNF) in sleep regulation was investigated by blocking TNF using a synthetic TNF receptor fragment (TNFRF) in rabbits. Intracerebroventricular injection of 50 micrograms TNFRF decreased spontaneous non-rapid-eye-movement (NREM) sleep across the 21 h recording period. After 6 h of sleep deprivation (SD), both duration of NREM sleep and electroencephalographic (EEG) slow wave activity during NREM sleep were enhanced. TNFRF suppressed SD-enhanced NREM sleep and EEG slow wave activity. SD per se did not affect rapid-eye-movement (REM) sleep, but TNFRF treatment increased REM sleep after SD. These results are consistent with the hypothesis that TNF is involved in the regulation of physiological NREM sleep.
Takahashi
S
S
Department of Physiology, University of Tennessee, Memphis 38163, USA.
Kapás
L
L
Seyer
J M
JM
Wang
Y
Y
Krueger
J M
JM
D000595:Amino Acid Sequence; D000818:Animals; D008297:Male; D008969:Molecular Sequence Data; D010446:Peptide Fragments; D017286:Polysomnography; D011817:Rabbits; D018124:Receptors, Tumor Necrosis Factor / Q000037:antagonists & inhibitors; D012890:Sleep / Q000187:drug effects*; D012892:Sleep Deprivation / Q000502:physiology; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors*
D010446:Peptide Fragments; D018124:Receptors, Tumor Necrosis Factor; D014409:Tumor Necrosis Factor-alpha; C101675:tumor necrosis factor receptor fragment (159-178)