Reactions of the Escherichia coli flavohaemoglobin (Hmp) with NADH and near-micromolar oxygen: oxygen affinity of NADH oxidase activity.
The soluble flavohaemoglobin (Hmp) of Escherichia coli, product of the hmp gene, contains haem B and FAD in a single polypeptide of molecular mass 44 kDa. The function of this protein (and of the similar proteins identified in several bacteria and yeast) is unknown, but the observation that the binding of oxygen to haem modulates the reduction level of FAD has suggested that Hmp could act as an oxygen sensor. Here, stopped-flow, rapid-scan spectroscopy has shown that the oxidized protein reacts rapidly with NADH to form an oxygenated species, even when efforts are made to reduce oxygen concentrations to sub-micromolar levels, suggesting a high affinity for this ligand. As is the case at high oxygen concentrations (130 microM), oxygenated species formation was kinetically and spectrally heterogeneous. Between 12 ms and 1 s after mixing, following transient formation of the deoxy form and its reaction with dioxygen, a steady-state level of the oxygenated species was attained. During the oxygenated steady state, the flavin remained largely oxidized, as observed previously at 130 microM oxygen. Hmp is an NADH oxidase; on exhaustion of oxygen by reduction (in < 10 s under these conditions), the oxygenated species disappeared to generate the deoxy Fe(II) haem, whereupon the flavin was reduced. The affinity for oxygen during NADH oxidation was measured by continuous dual-wavelength monitoring of the deoxygenation of oxymyoglobin. The Km for oxygen was 2.6 microM, much higher than the Km values determined, using the same method, for the membrane-bound terminal oxidases cytochromes bo' and bd. These results show that the oxidase activity of Hmp, but not necessarily oxygen binding, would be minimal at oxygen concentrations that limit terminal oxidase function.
Poole
Robert K
RK
Division of Life Sciences, King's College London, Campden Hill Road, London W8 7AH, UK.
Ioannidis
Nikolaos
N
Division of Life Sciences, King's College London, Campden Hill Road, London W8 7AH, UK.
Orii
Yutaka
Y
Department of Public Health, Graduate School of Medicine, Kyoto University, Kyoto 606, Japan.
D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism*; D004093:Dihydropteridine Reductase; D004926:Escherichia coli / Q000378:metabolism*; D029968:Escherichia coli Proteins; D006420:Hemeproteins / Q000737:chemistry / Q000378:metabolism*; D007700:Kinetics; D009097:Multienzyme Complexes / Q000378:metabolism*; D009243:NAD / Q000378:metabolism*; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism*; D010084:Oxidation-Reduction; D010100:Oxygen / Q000378:metabolism*; D011485:Protein Binding; D013045:Species Specificity
D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D006420:Hemeproteins; D009097:Multienzyme Complexes; C100771:Vgb protein, Bacteria; D009243:NAD; D004093:Dihydropteridine Reductase; C076538:hmp protein, E coli; C023107:NADH oxidase; D009247:NADH, NADPH Oxidoreductases; D010100:Oxygen