Application of polymerase chain reaction with arbitrary primers to strain identification of Mycoplasma gallisepticum.
DNA heterogeneity among strains and isolates of Mycoplasma gallisepticum (MG) was demonstrated with the arbitrarily primed polymerase chain reaction (AP-PCR) method. This method involves three cycles of low-stringency amplification followed by PCR at higher stringency. Reproducible DNA fragments of 25 different MG strains or isolates were generated with three arbitrarily chosen primers. The MG strains or isolates were distinguished according to the banding patterns of their amplified DNA on agarose gels, and the differences were characteristic for specific isolates. This method is rapid, simple, and reproducible, and it can also be used to determine the similarity between isolates of MG from various sources.
Fan
H H
HH
Department of Avian Medicine, University of Georgia, Athens 30602-4875, USA.
Kleven
S H
SH
Jackwood
M W
MW
D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000382:microbiology; D002645:Chickens; D017931:DNA Primers; D004587:Electrophoresis, Agar Gel; D008969:Molecular Sequence Data; D009174:Mycoplasma / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D009175:Mycoplasma Infections / Q000175:diagnosis / Q000662:veterinary*; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D011110:Polymorphism, Genetic; D011201:Poultry Diseases; D015203:Reproducibility of Results; D014132:Trachea / Q000382:microbiology; D014422:Turkeys
D017931:DNA Primers