Human cytochrome P450's are pro-oxidants in iron/ascorbate-initiated microsomal lipid peroxidation.
We have examined the effect of human cytochrome P450's (1A1,1A2,3A4,2A6,2B6,2D6,2E1) on ascorbate/iron-induced lipid peroxidation. Using microsomes prepared from human lymphoblastic cells enriched in recombinant cytochrome P450 isoenzymes, we have shown that the degree of peroxidation is a function of the amount of P450 present rather than the presence of any specific isoenzyme. Incorporated P450 increased the amount of peroxidation products by up to 2.1-fold compared to the control microsomes with no P450. It is therefore concluded that cytochrome P450's play a significant role in ascorbate/iron peroxidation.
Lambert
N
N
Department of Food Molecular Biochemistry, Norwich Research Park, Colney, UK.
Chambers
S J
SJ
Plumb
G W
GW
Williamson
G
G
D001205:Ascorbic Acid / Q000494:pharmacology*; D001402:B-Lymphocytes / Q000201:enzymology; D002248:Carbon Monoxide / Q000494:pharmacology; D019363:Cytochrome P-450 CYP1A1; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D003576:Electron Transport Complex IV / Q000378:metabolism*; D005296:Ferrous Compounds / Q000494:pharmacology*; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism; D015227:Lipid Peroxidation; D008861:Microsomes / Q000187:drug effects / Q000378:metabolism*; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism*; D016877:Oxidants; D010088:Oxidoreductases / Q000378:metabolism
D005296:Ferrous Compounds; D007527:Isoenzymes; D016877:Oxidants; C051140:iron(II)-ascorbic acid complex; D002248:Carbon Monoxide; D003577:Cytochrome P-450 Enzyme System; D010088:Oxidoreductases; D019363:Cytochrome P-450 CYP1A1; D003576:Electron Transport Complex IV; D001205:Ascorbic Acid