Changes in M-CSF-like activity during chicken embryonic development.
We have established a method for separation of chicken bone-marrow cells using Percoll density gradient centrifugation, and have developed a new method for determining chicken M-CSF-like activity employing a liquid culture. Using this method, we determined M-CSF-like activities in egg yolk, chorioallantoic fluid (CAF) and amniotic fluid (AmF), and studied the effects of M-CSF on development of chicken embryos. M-CSF-like activity in egg yolk was at a high level before the incubation of the egg; it began to decrease on the third day of incubation and rapidly decreased on the fourth day, and no significant activity was detected after the tenth day of incubation. M-CSF-like activity in CAF was very low, and it exhibited almost no change during development. No M-CSF-like activity was detected in AmF throughout the experimental period.
Shao
X
X
Department of Microbiology and Immunology, Nippon Medical School, Tokyo, Japan.
Kikuchi
K
K
Watari
E
E
Norose
Y
Y
Araki
T
T
Yokomuro
K
K
D000482:Allantois / Q000378:metabolism*; D000653:Amniotic Fluid / Q000378:metabolism*; D000818:Animals; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D002642:Chick Embryo; D002823:Chorion / Q000378:metabolism*; D003102:Colloids; D004530:Egg Yolk / Q000378:metabolism*; D016173:Macrophage Colony-Stimulating Factor / Q000097:blood / Q000378:metabolism*; D011205:Povidone; D012822:Silicon Dioxide
D003102:Colloids; C016039:Percoll; D012822:Silicon Dioxide; D016173:Macrophage Colony-Stimulating Factor; D011205:Povidone