title: SV40-mediated immortalization of human fibroblasts.
abstract: We have identified a multistep mechanism by which the DNA virus SV40 overcomes cellular senescence. Expression of SV40 T antigen is required for both transient extension of life span and unlimited life span or immortalization. These effects are mediated through inactivation of function of growth suppressors pRB and p53 via complex formation with T antigen. However, immortalization additionally requires inactivation of a novel growth suppressor gene, which has recently been identified to be on the distal portion of the long arm of chromosome 6, designated SEN6. We propose that SEN6 is responsible for cellular senescence in fibroblasts and other cells.
authors.affiliation: Department of Microbiology and Molecular Genetics, UMDNJ-New Jersey Medical School, Newark 07103, USA.
authors.forename: H L
authors.initials: HL
authors.lastname: Ozer
authors.forename: S S
authors.initials: SS
authors.lastname: Banga
authors.forename: T
authors.initials: T
authors.lastname: Dasgupta
authors.forename: J
authors.initials: J
authors.lastname: Houghton
authors.forename: K
authors.initials: K
authors.lastname: Hubbard
authors.forename: K K
authors.initials: KK
authors.lastname: Jha
authors.forename: S H
authors.initials: SH
authors.lastname: Kim
authors.forename: M
authors.initials: M
authors.lastname: Lenahan
authors.forename: Z
authors.initials: Z
authors.lastname: Pang
authors.forename: J R
authors.initials: JR
authors.lastname: Pardinas
authors.forename: P C
authors.initials: PC
authors.lastname: Patsalis
mesh_terms: D000952:Antigens, Polyomavirus Transforming / Q000502:physiology; D002472:Cell Transformation, Viral; D016922:Cellular Senescence; D005347:Fibroblasts; D016147:Genes, Tumor Suppressor; D006801:Humans; D013539:Simian virus 40 / Q000235:genetics*; D016159:Tumor Suppressor Protein p53 / Q000502:physiology
chemical_list: D000952:Antigens, Polyomavirus Transforming; D016159:Tumor Suppressor Protein p53