Esterase coupled with the H2O2/horseradish peroxidase system triggers chemiluminescence from 2-methyl-1-propenylbenzoate: a potential analytical tool for esterase analysis.
The hydrolysis of 2-methyl-1-propenylbenzoate catalyzed by esterase produces 2-methyl-1-propenol, which can be subsequently oxidized by the H2O2/horseradish peroxidase (HRP) system to yield electronically excited triplet acetone. The level of luminescence elicited by this species is proportional to total esterase used, making it possible to determine as little as 2 pmol of esterase. Yet, its intensity can be enhanced several orders of magnitude by fluorescent acceptors like sodium 9,10-dibromoanthracene-2-sulfonate. The system works as a chemiluminescent reaction triggered by esterase and can be used to elaborate analytical assays to determine its activity. This chemiluminescence is also promoted by HRP conjugates instead of free HRP and, hence, this simple reaction system can also be used to develop sensitive chemiluminescent immunoassays based upon peroxidase activity.
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Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, Brazil.
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D000447:Aldehydes; D000818:Animals; D001565:Benzoates / Q000737:chemistry*; D002384:Catalysis; D004285:Dogs; D004950:Esterases / Q000032:analysis* / Q000737:chemistry; D006735:Horseradish Peroxidase / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000737:chemistry*; D006868:Hydrolysis; D007074:Immunoglobulin G / Q000737:chemistry; D008099:Liver / Q000201:enzymology*; D008163:Luminescent Measurements
C101261:2-methyl-1-propenylbenzoate; D000447:Aldehydes; D001565:Benzoates; D007074:Immunoglobulin G; D006861:Hydrogen Peroxide; C017439:isobutyraldehyde; D006735:Horseradish Peroxidase; D004950:Esterases