Regulation of the C3 production by gamma interferon from peripheral blood T cells in patients with membranoproliferative glomerulonephritis and poststreptococcal acute glomerulonephritis.
This study assessed the regulatory role of peripheral blood T cells in the C3 production in patients with poststreptococcal glomerulonephritis and branoproliferative glomerulonephritis. Peripheral blood T cells from patients at various stages of disease were cultured and the supernatants tested for gamma interferon (IFN-gamma) content and the capacity to stimulate C3 production by HuH-7 cells. Supernatants from patients with membranoproliferative glomerulonephritis and from convalescent patients with poststreptococcal glomerulonephritis significantly stimulated the C3 production; the degree of stimulation correlated with the IFN-gamma content of the supernatants. Similar results were obtained using recombinant IFN-gamma. In both cases, the effect was blocked by the addition of anti-IFN-gamma monoclonal antibody to the cultures. Interleukin 2 and interleukin 6 levels in supernatants from T cell cultures of patients and controls were essentially the same. In summary, IFN-gamma plays a regulatory role in C3 production by human hepatoma cell lines.
Yan
K
K
Department of Pediatrics, Kyorin University School of Medicine, Tokyo, Japan.
Nakamura
Y
Y
Watanabe
N
N
Kawato
H
H
Fujimiya
Y
Y
Kohsaka
T
T
D000208:Acute Disease; D000293:Adolescent; D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D002648:Child; D003176:Complement C3 / Q000096:biosynthesis*; D003470:Culture Media; D016207:Cytokines / Q000096:biosynthesis; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005921:Glomerulonephritis / Q000209:etiology / Q000378:metabolism*; D015432:Glomerulonephritis, Membranoproliferative / Q000378:metabolism*; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008114:Liver Neoplasms, Experimental / Q000378:metabolism; D008297:Male; D013290:Streptococcal Infections / Q000150:complications*; D013601:T-Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D000998:Antiviral Agents; D003176:Complement C3; D003470:Culture Media; D016207:Cytokines; D007371:Interferon-gamma