The effect of dietary supplements on gene expression in mice tissues.
Exposure of living organisms to various environmental stresses induces the synthesis of so-called shock/stress proteins; many of them can provide either immediate stress protection or participate in cellular repair processes. In the present study we focused our attention on the potential effect of dietary vitamins and microelements with antioxidant properties on stress protein gene expression. The analysis of gene expression in tissues of antioxidant-fed mice shows hsp-70 gene overexpression in liver and brain, but not in spleen and lung. Heat shock significantly induces gene expression that is less pronounced in antioxidant-fed animals in all analyzed tissues. Under conditions of oxidative stress, accumulation of lipid peroxidation products in liver homogenates is partially suppressed in mice subjected to heat shock, and significantly inhibited in antioxidant-fed mice and in antioxidant-fed mice subjected to heat shock. The glutathione content in liver homogenates of antioxidant-fed mice is higher than in the control group. Heat shock decreases the level of endogenous glutathione in both groups of animals, but it is still higher in the liver homogenate of antioxidant-fed mice. Thus, dietary supplements can modify gene expression induced by heat shock in vivo and protect rat tissues against oxidative stress by enhancing the level of endogenous antioxidants and inducing hsp-70 gene expression.
Ushakova
T
T
Institute of Theoretical and Experimental Biophysics, Pushchino, Russia.
Melkonyan
H
H
Nikonova
L
L
Mudrik
N
N
Gogvadze
V
V
Zhukova
A
A
Gaziev
A I
AI
Bradbury
R
R
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001483:Base Sequence; D017931:DNA Primers; D005527:Food, Fortified; D015870:Gene Expression / Q000187:drug effects; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis*; D006358:Hot Temperature; D008099:Liver / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D013154:Spleen / Q000378:metabolism; D013312:Stress, Physiological; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis; D014158:Transcription, Genetic
D000975:Antioxidants; D017931:DNA Primers; D018840:HSP70 Heat-Shock Proteins; D017392:Thiobarbituric Acid Reactive Substances