Chronic effects of somatotropin treatment on response of subcutaneous adipose tissue lipolysis to acutely acting factors in vivo and in vitro.
The basis of the chronic lipolytic effect of somatotropin on adipose tissue was investigated in sheep. Lipolytic rate was assessed in subcutaneous adipose tissue both in vivo, by microdialysis, and in vitro. Somatotropin treatment resulted in a small increase in basal (unstimulated) lipolysis and also in the maximum lipolytic rate observed in the presence of catecholamines both in vivo and in vitro. There was a small increase in the number of beta-adrenergic receptors but no change in the amount of the two isoforms of the stimulatory GTP-binding protein, Gs. Treatment with somatotropin decreased the response to antilipolytic agents such as the adenosine analog N6-phenylisopropyladenosine and prostaglandin E1. There was, however, no change in the number of adenosine receptors or amounts of the inhibitory GTP-binding proteins (Gi-1 plus Gi-2). Somatotropin also decreased prostaglandin E2 production by subcutaneous adipose tissue in vivo. Somatotropin treatment thus alters lipolytic regulation in sheep and this is characterized by changes in a number of proteins involved in this process.
Doris
R A
RA
Hannah Research Institute, Ayr, Scotland, U.K.
Thompson
G E
GE
Finley
E
E
Kilgour
E
E
Houslay
M D
MD
Vernon
R G
RG
D017667:Adipocytes / Q000737:chemistry / Q000648:ultrastructure; D000273:Adipose Tissue / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D000818:Animals; D002395:Catecholamines / Q000494:pharmacology; D002462:Cell Membrane / Q000737:chemistry / Q000648:ultrastructure; D005260:Female; D019204:GTP-Binding Proteins / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D005990:Glycerol / Q000097:blood; D013006:Growth Hormone / Q000494:pharmacology*; D066298:In Vitro Techniques; D007536:Isomerism; D008066:Lipolysis / Q000187:drug effects* / Q000502:physiology; D011942:Receptors, Adrenergic, alpha / Q000032:analysis / Q000187:drug effects / Q000378:metabolism; D011943:Receptors, Adrenergic, beta / Q000032:analysis / Q000187:drug effects / Q000378:metabolism; D012756:Sheep / Q000378:metabolism* / Q000502:physiology
D002395:Catecholamines; D011942:Receptors, Adrenergic, alpha; D011943:Receptors, Adrenergic, beta; D013006:Growth Hormone; D019204:GTP-Binding Proteins; D005990:Glycerol