Effect of recombinant growth hormone and chromium picolinate on cytokine production and growth performance in swine.
The effect of dietary chromium picolinate (CrP) and recombinant porcine growth hormone, somatotropin (rPST) administration on growth performance and cytokine production in Landrace-Poland China gilts was determined using a 2 by 2 treatment array. Treatments were: (1) control (basal diet), (2) CrP-supplemented diet (basal diet + 300 micrograms Cr3+/kg diet as CrP), (3) rPST (100 pg/kg body weight/day), and (4) rPST+CrP. CrP-supplemented diets were fed beginning at 20 kg body weight through 90 kg. Administration of rPST was begun at 60 kg weight and continued through 90 kg. All rPST treated pigs demonstrated improvements in growth performance versus controls. Pigs given CrP-supplemented diets showed no differences in growth performance. At 90 kg, pigs were challenged with endotoxin (lipopolysaccharide, 0.2 microgram/kg i.v.). Blood samples were collected at 0, 1, and 3 h postchallenge. Plasma IL-6 levels increased from 23 U/ml at time 0 to 1,927 U/ml at 3 h for control swine. Swine from the CrP treatment group had IL-6 levels of 8,130 U/ml at 3 h post-LPS. There were no differences in plasma IL-6 from pigs in the rPST and rPST+CrP treatment groups compared to the controls. Endotoxin challenge had no effect on either blood glucose levels or induction of TNF-alpha in any treatment group. PBMC from CrP-treated animals produced more IL-2 than peripheral blood mononuclear cells from all other groups.
Myers
M J
MJ
Animal Biology Branch, US FDA, Beltsville, MD 20705, USA.
Farrell
D E
DE
Evock-Clover
C M
CM
Cope
C V
CV
Henderson
M
M
Steele
N C
NC
D000818:Animals; D001823:Body Composition / Q000187:drug effects; D001835:Body Weight / Q000187:drug effects; D016207:Cytokines / Q000096:biosynthesis* / Q000187:drug effects*; D004032:Diet; D013006:Growth Hormone / Q000008:administration & dosage / Q000494:pharmacology*; D007502:Iron Chelating Agents / Q000494:pharmacology*; D010848:Picolinic Acids / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000494:pharmacology*; D013552:Swine / Q000254:growth & development*
D016207:Cytokines; D007502:Iron Chelating Agents; D010848:Picolinic Acids; D011994:Recombinant Proteins; D013006:Growth Hormone; C030614:picolinic acid