title: Fungal ferritins: the ferritin from mycelia of Absidia spinosa is a bacterioferritin.
abstract: Two distinct ferritin like iron containing proteins have been identified and isolated from the fungus Absidia spinosa; one from the spores and another from the mycelia. The mycelial protein has been purified and consists of two subunits of approx. 20 kDa. The N-terminal sequences of both subunits have been determined. The holoprotein as isolated contains approx. 750 iron atoms/molecule and exhibits a heme-like UV-Vis spectrum. Based on the heme spectrum and the high degree of sequence homology found, it has been established that the mycelial protein is a bacterioferritin. This is the first example demonstrating the presence of a bacterioferritin in a eukaryotic organism.
authors.affiliation: University of Tübingen, Germany. cc05@swt.edu
authors.forename: C J
authors.initials: CJ
authors.lastname: Carrano
authors.forename: R
authors.initials: R
authors.lastname: Böhnke
authors.forename: B F
authors.initials: BF
authors.lastname: Matzanke
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D003573:Cytochrome b Group / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D005293:Ferritins / Q000737:chemistry* / Q000302:isolation & purification; D005656:Fungal Proteins / Q000737:chemistry; D006420:Hemeproteins / Q000737:chemistry; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009090:Mucorales / Q000737:chemistry*; D017386:Sequence Homology, Amino Acid; D015204:Spectroscopy, Mossbauer; D013172:Spores, Fungal / Q000737:chemistry
chemical_list: D001426:Bacterial Proteins; D003573:Cytochrome b Group; D005656:Fungal Proteins; D006420:Hemeproteins; D005293:Ferritins; C018032:bacterioferritin