Production of metal dependent beta-lactamases by clinical strains of Bacteroides fragilis isolated before 1987.
Five imipenem resistant clinical isolates of Bacteroides fragilis isolated before 1987, were examined to determine if they produced metallo-beta-lactamases. The beta-lactamases produced by the clinical isolates all focused as doublet bands at pl 4.8/4.9, characteristic of the B. fragilis CfiA type metallo-beta-lactamase. Each enzyme had a similar substrate profile and were inhibited by EDTA and activated with zinc sulphate. The sequence of the metallo-beta-lactamase gene from B. fragilis ED262 was determined and confirmed to be a CfiA type beta-lactamase. Consequently, the five isolates examined probably produce a CfiA type beta-lactamase, suggesting that metallo-beta-lactamases were present before the widespread use of carbapenems.
Khushi
T
T
SmithKline Beecham Pharmaceuticals, Betchworth, Surrey, UK.
Payne
D J
DJ
Fosberry
A
A
Reading
C
C
D001441:Bacteroides fragilis / Q000302:isolation & purification / Q000378:metabolism*; D001483:Base Sequence; D015378:Imipenem / Q000494:pharmacology*; D008670:Metals / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D012189:Retrospective Studies; D013379:Substrate Specificity; D013845:Thienamycins / Q000494:pharmacology*; D006113:United Kingdom; D001618:beta-Lactamases / Q000096:biosynthesis*
D008670:Metals; D013845:Thienamycins; D015378:Imipenem; D001618:beta-Lactamases