title: [Immunohistochemical study of 5-HT on gunshot wound of human skin].
abstract: Fourty antemortem and 30 postmortem gunshot wound samples of human skin were studied by immunohistochemical method. The 5-HT was seen mainly in wound edge, papillary layer, hypodermis and surrounding tissue of all antemortem gunshot wounds. The 5-HT was also discovered on the postmortem gunshot wounds which occurred within 8 minutes after death. The result showed that immunohistochemical staining of 5-HT be useful for diagnosing the antemortem gunshot wound. It also demonstrated that the mast cells of human do not contain 5-HT. We also studied the rate of mast cell degranulation of gunshot wounds of human skin by toluidine blue staining. The rate of mast cell degranulation of antemortem gunshot wounds increased (50%), and it was higher than that of postmortem gunshot wounds, suggesting that the rising of degranulation rate is a sign of antemortem injury.
authors.affiliation: Department of Forensic Pathology, Chongqing University of Medical Sciences.
authors.forename: M
authors.initials: M
authors.lastname: Liu
authors.forename: J
authors.initials: J
authors.lastname: Wu
authors.forename: Z
authors.initials: Z
authors.lastname: Zhang
authors.forename: J
authors.initials: J
authors.lastname: Li
mesh_terms: D015550:Cell Degranulation; D005554:Forensic Medicine; D006801:Humans; D007150:Immunohistochemistry; D008407:Mast Cells / Q000502:physiology; D011180:Postmortem Changes; D012701:Serotonin / Q000378:metabolism*; D012867:Skin / Q000293:injuries* / Q000378:metabolism*; D014948:Wounds, Gunshot / Q000378:metabolism*
chemical_list: D012701:Serotonin