title: Evaluation of different absorbance ratios from infrared spectroscopy for analyzing the degree of deacetylation in chitin.
abstract: Various acetylated chitosan derivatives and mixtures of chitin and chitosan, covering the range of the degree of deacetylation (DDA) from 0-100% were analyzed by 1H-NMR spectroscopy and infrared spectroscopy. The use of the 1070 cm-1 or 1030 cm-1 absorption band as an internal standard in the determination of DDA from the absorbance of the amide I bands at 1655 cm-1 and 1630 cm-1 or the amide II band at 1560 cm-1 was studied. There is a good correlation between the results from IR spectroscopy and those from 1H-NMR spectroscopy.
authors.affiliation: Department of Materials Science, Faculty of Engineering, Tottori University, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Shigemasa
authors.forename: H
authors.initials: H
authors.lastname: Matsuura
authors.forename: H
authors.initials: H
authors.lastname: Sashiwa
authors.forename: H
authors.initials: H
authors.lastname: Saimoto
mesh_terms: D000107:Acetylation; D000818:Animals; D003386:Brachyura / Q000737:chemistry; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002686:Chitin / Q000031:analogs & derivatives* / Q000737:chemistry*; D048271:Chitosan; D005069:Evaluation Studies as Topic; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D013055:Spectrophotometry, Infrared
chemical_list: D002686:Chitin; D048271:Chitosan