Glycosyl inositolphospholipid-anchored structures in Herpetomonas davidi.
Glycosyl inositolphospholipid (GPI)-anchored structures in the monogenetic parasite Herpetomanas davidi, were labeled with [3H]glucosamine, and characterized by enzymatic and chemical treatments that are typical for the identification of GPI anchors. [3H]Myristate incorporated into two different pools of GPI-linked structures that could be separated by chromatography on octyl-Sepharose. One pool consisted of three GPI-anchored proteins with apparent molecular masses of 21,31 and 45 kDa, and the GPI lipid moieties were identified as alkyl-lysoglycerols. The label in the other pool associated with lipopeptidophosphoglycan (LPPG)-like structures of approximately 12-kDa molecular mass, containing ceramide-type GPI lipid anchors. While protein GPI anchors could also be labeled using [3H]glucosamine as radiolabeled GPI anchor precursor, hardly any radioactivity was incorporated into the LPPG-like structures. H. davidi is one of the few organisms identified to date that synthesizes two structurally different lipid moieties for GPI anchoring of membrane components.
Bütikofer
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Institute of Biochemistry & Molecular Biology, University of Bern, Switzerland. buetikofer@mci.unibe.ch
Boschung
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D000818:Animals; D005227:Fatty Acids / Q000032:analysis; D005944:Glucosamine / Q000378:metabolism; D017261:Glycosylphosphatidylinositols / Q000737:chemistry / Q000378:metabolism*; D007303:Insect Vectors / Q000469:parasitology; D015394:Molecular Structure; D008970:Molecular Weight; D019814:Myristic Acid; D009227:Myristic Acids / Q000378:metabolism; D010457:Peptidoglycan / Q000737:chemistry / Q000378:metabolism; D010743:Phospholipids / Q000737:chemistry / Q000378:metabolism; D015800:Protozoan Proteins / Q000737:chemistry / Q000378:metabolism; D014351:Trypanosomatina / Q000378:metabolism*
D005227:Fatty Acids; D017261:Glycosylphosphatidylinositols; D009227:Myristic Acids; D010457:Peptidoglycan; D010743:Phospholipids; D015800:Protozoan Proteins; C014643:lipopeptidophosphoglycan; D019814:Myristic Acid; D005944:Glucosamine