title: Dendritic cell presentation of PPD and 19 kDa protein of Mycobacterium tuberculosis and emergent T helper cell phenotype.
abstract: Protection against infection with Mycobacterium tuberculosis is preferentially associated with the development of the T helper 1 subset, IFN-gamma production and a cell-mediated response, rather than with T helper 2 cells, 4 (IL-4) and antibody production. The type of APC interacting with T cells responsive to mycobacterial peptides may influence which of these responses predominates. This investigation focuses on the role of dendritic cells (DC) because they are the most potent APC in both primary and recall immune responses. Our results show that splenic DC-enriched suspensions prepared from C57BL/6 mice and pulsed with either purified protein derivative (PPD) or the immunodominant 19 kDa protein from M. tuberculosis, can activate antigen-primed T cells in vitro, whereas spleen cell suspensions depleted of DC cannot. DC pulsed with PPD or 19 kDa antigen are able to prime naive T cells in vivo. Supernatants collected from cultures containing T cells from mice injected with PPD-pulsed DC and then challenged in vitro with PPD-pulsed DC were found to contain more IL-2 and IFN-gamma than those from control mice which received either DC or PPD alone. No such antigen-specific IFN-gamma response occurred if DC pulsed with 19 kDa were used in place of PPD-pulsed DC. IL-4 was not detected in any of the culture supernatants. We conclude that DC can induce production of cytokines associated with a protective immune response when presenting peptides derived from heterogeneous mycobacterial antigens but not when exposed to the single 19 kDa immunodominant protein.
authors.affiliation: Department of Pathology, University of Otago, Dunedin, New Zeland.
authors.forename: M A
authors.initials: MA
authors.lastname: Baird
authors.forename: D N
authors.initials: DN
authors.lastname: Hart
authors.forename: N
authors.initials: N
authors.lastname: Abernethy
authors.forename: J D
authors.initials: JD
authors.lastname: Watson
mesh_terms: D000818:Animals; D017951:Antigen Presentation; D001426:Bacterial Proteins / Q000276:immunology*; D003713:Dendritic Cells / Q000276:immunology*; D007371:Interferon-gamma / Q000096:biosynthesis; D007376:Interleukin-2 / Q000096:biosynthesis; D008213:Lymphocyte Activation; D051379:Mice; D008810:Mice, Inbred C57BL; D009169:Mycobacterium tuberculosis / Q000737:chemistry; D013154:Spleen / Q000166:cytology*; D018418:Th2 Cells / Q000276:immunology*; D014373:Tuberculin / Q000276:immunology*
chemical_list: C087864:19 kDa antigen, Mycobacterium; D001426:Bacterial Proteins; D007376:Interleukin-2; D014373:Tuberculin; D007371:Interferon-gamma