Cell type specific regulation of COUP-TF II promoter activity.
COUP-TF family orphan receptors regulate activity of ligand-activated nuclear hormone receptors or function independently in the regulation of gene expression. COUP-TF II has a complex expression pattern suggesting that different mechanisms are involved in the regulation of its expression. We isolated the 5' regulatory region of the mouse COUP-TF II gene and demonstrated that the basal promoter is localized in a -200 bp region 5' from the transcription start site. All-trans retinoic acid and dibutyryl cyclic AMP have cell type specific effects on COUP-TF II promoter activity. The effect of cyclic AMP is mediated by the cyclic AMP response element that is localized 74 nucleotides upstream from the major transcriptional start. In vitro promoter analyses also demonstrated that the effect of all-trans RA is not directly mediated by the binding of RARs or RXRs to the promoter sequence.
Soosaar
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Department of Anatomy and Neurobiology, Colorado State University, Fort Collins 80523, USA.
Neuman
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Nornes
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Neuman
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D000818:Animals; D001483:Base Sequence; D003994:Bucladesine / Q000494:pharmacology; D051838:COUP Transcription Factor I; D051836:COUP Transcription Factors; D018236:Carcinoma, Embryonal; D015500:Chloramphenicol O-Acetyltransferase / Q000096:biosynthesis; D015342:DNA Probes; D018076:DNA, Complementary; D004268:DNA-Binding Proteins / Q000378:metabolism; D005786:Gene Expression Regulation; D015698:Genomic Library; D051379:Mice; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D011401:Promoter Regions, Genetic; D011956:Receptors, Cell Surface / Q000096:biosynthesis / Q000235:genetics; D011987:Receptors, Steroid; D011994:Recombinant Proteins / Q000096:biosynthesis; D012045:Regulatory Sequences, Nucleic Acid; D015183:Restriction Mapping; D014157:Transcription Factors / Q000096:biosynthesis* / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic / Q000187:drug effects; D014162:Transfection; D014212:Tretinoin / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D051838:COUP Transcription Factor I; D051836:COUP Transcription Factors; D015342:DNA Probes; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; C497590:Nr2f1 protein, mouse; D011956:Receptors, Cell Surface; D011987:Receptors, Steroid; D011994:Recombinant Proteins; D014157:Transcription Factors; D014212:Tretinoin; D003994:Bucladesine; D015500:Chloramphenicol O-Acetyltransferase