Use of excretory/secretory antigens for the serodiagnosis of Anoplocephala perfoliata cestodosis.
Whole worm extract (WWE) and excretory/secretory (E/S) antigens of Anoplocephala perfoliata were characterised by SDS-PAGE and their use in the serodiagnosis of equine cestodosis was evaluated. An enzyme-linked immunosorbent assay (ELISA) was used to compare WWE and E/S antigen as the capture layer in an antibody capture ELISA. E/S antigen gave the best differentiation between sera from tapeworm-positive and tapeworm-negative horses. The E/S-ELISA was optimised and validated against sera from horses of known tapeworm status. This assay gave a diagnostic sensitivity of 68% (n = 38) and a specificity in helminth-naive horses of 95% (n = 20). Western blot analysis of tapeworm-positive and tapeworm-negative sera revealed the immunoreactive proteins of E/S antigen to be those of 12 and 13 kDa.
Proudman
C J
CJ
Liverpool School of Tropical Medicine/Faculty of Veterinary Science, University of Liverpool, UK.
Trees
A J
AJ
D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D000918:Antibody Specificity; D000947:Antigens, Helminth / Q000097:blood*; D002589:Cestoda / Q000276:immunology / Q000302:isolation & purification; D002590:Cestode Infections / Q000175:diagnosis / Q000662:veterinary*; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary; D006734:Horse Diseases; D006736:Horses; D015151:Immunoblotting; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000379:methods; D013045:Species Specificity
D000909:Antibodies, Helminth; D000947:Antigens, Helminth