Construction and characterization of a deletion mutant of gpd2 that encodes an isozyme of NADH-dependent glycerol-3-phosphate dehydrogenase in fission yeast.
Schizosaccharomyces pombe has two genes each encoding an isozyme of NADH-dependent glycerol-3-phosphate dehydrogenases (gpd1+ and gpd2+). To gain an insight into the function of these genes, here we constructed a gpd2 deletion mutant, in addition to the previously constructed gpd1 deletion mutant. We showed that the gpd1+ and gpd2+ gene-products are both functional in terms of the de novo glycerol synthesis. Furthermore, the gpd1(+)-mediated glycerol production is primarily responsible for the osmoregulation, but the gpd2+ gene is not. Interestingly, however, the gpd2 deletion mutant had histidine- or lysine-auxotrophy for growth on a minimal medium.
Yamada
H
H
Laboratory of Molecular Microbiology, School of Agriculture, Nagoya University, Japan.
Ohmiya
R
R
Aiba
H
H
Mizuno
T
T
D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic / Q000235:genetics; D005990:Glycerol / Q000378:metabolism; D005993:Glycerolphosphate Dehydrogenase / Q000235:genetics*; D006639:Histidine / Q000737:chemistry; D007527:Isoenzymes / Q000235:genetics*; D008239:Lysine / Q000737:chemistry; D009154:Mutation / Q000235:genetics*; D009243:NAD / Q000378:metabolism*; D009994:Osmolar Concentration; D010641:Phenotype; D012568:Schizosaccharomyces / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development
D004271:DNA, Fungal; D007527:Isoenzymes; D009243:NAD; D006639:Histidine; D005993:Glycerolphosphate Dehydrogenase; D008239:Lysine; D005990:Glycerol