title: Fibrinogen promotes adhesion of monocytic to human mesothelioma cells.
abstract: Adhesion between monocytic and mesothelioma or pleural mesothelial cells influences stromal remodeling in pleural neoplasia. We found that cultured monocytic cells (U937) adhere to either human pleural mesothelioma (MS-1) or mesothelial (MeT5A) cells in vitro. 125I-fibrinogen bound specifically and saturably to either cell line, and specific fibrinogen binding increased upon stimulation of these cells with proinflammatory agents such as phorbol myristate (PMA), lipopolysaccharide (LPS) or tumor necrosis factor (TNF-alpha). We purified the fibrinogen receptor protein from a membrane fraction of MS-1 cells and identified it by immunoprecipitation as intercellular adhesion molecule (ICAM-1). Anti-ICAM-1 antibody or antisense oligonucleotides inhibited fibrinogen-mediated cell adhesion and binding of 125I-fibrinogen to mesothelioma or mesothelial cells. Cultured monocytic cells adhere to either mesothelioma or mesothelial cells, and the interaction is promoted by fibrinogen binding ICAM-1 at the cell surface. ICAM-1 is expressed by mesothelioma cells and CD 11b by macrophages in the fibrinous mesothelioma tumor stroma. The data suggest a common mechanism by which monocytic cells could adhere to either malignant mesothelioma cells or the mesothelial surface in pleural neoplasia.
authors.affiliation: Department of Medicine, University of Texas Health Center of Tyler 75710, USA.
authors.forename: S
authors.initials: S
authors.lastname: Shetty
authors.forename: A
authors.initials: A
authors.lastname: Kumar
authors.forename: S
authors.initials: S
authors.lastname: Pueblitz
authors.forename: S
authors.initials: S
authors.lastname: Emri
authors.forename: Y
authors.initials: Y
authors.lastname: Gungen
authors.forename: A R
authors.initials: AR
authors.lastname: Johnson
authors.forename: S
authors.initials: S
authors.lastname: Idell
mesh_terms: D001665:Binding Sites; D002448:Cell Adhesion / Q000187:drug effects; D005340:Fibrinogen / Q000378:metabolism / Q000494:pharmacology*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D008654:Mesothelioma / Q000378:metabolism / Q000473:pathology*; D010997:Pleural Neoplasms / Q000378:metabolism / Q000473:pathology*; D011869:Radioligand Assay; D014407:Tumor Cells, Cultured
chemical_list: D018799:Intercellular Adhesion Molecule-1; D005340:Fibrinogen