Structural analysis of the O-antigenic polysaccharide from the enteropathogenic Escherichia coli O125.
The structure of the polysaccharide part of the lipopolysaccharide obtained from the enteropathogenic Escherichia coli O125 has been investigated. Methylation analysis, 1H-NMR spectroscopy and 13C-NMR spectroscopy revealed that the polysaccharide is composed of repeating hexasaccharide units. Smith degradation of the native O-polysaccharide resulted in a polysaccharide with four sugar residues in the repeating unit. Information on the sequence of the native O-polysaccharide and the Smith-degraded product was obtained by two-dimensional techniques, namely heteronuclear-multiple-bond-connectivity and NOESY experiments. The structure of the repeating unit of the O-polysaccharide of E. coli strain O125, which has two adjacent branch-point residues, is [sequence: see text].
Kjellberg
A
A
Department of Organic Chemistry, Arrhenius Laboratory, Stockholm University, Sweden.
Urbina
F
F
Weintraub
A
A
Widmalm
G
G
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000032:analysis; D002247:Carbon Isotopes; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000737:chemistry* / Q000276:immunology / Q000472:pathogenicity; D006859:Hydrogen; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D008969:Molecular Sequence Data; D019081:O Antigens / Q000737:chemistry* / Q000302:isolation & purification
D002241:Carbohydrates; D002247:Carbon Isotopes; D019081:O Antigens; D006859:Hydrogen