Apoptosis in cell cultures induced by infectious bursal disease virus following in vitro infection.
Chicken embryo fibroblasts (CEFs) and Vero cells infected with infectious bursal disease virus (IBDV) exhibited the biochemical feature of apoptosis. Agarose gel electrophoresis of DNA extracted from IBDV-infected cells revealed the characteristic laddering pattern of DNA fragmentation, which was more intense in infected CEFs than in Vero cells. The appearance of apoptotic nucleosomal DNA fragments in IBDV-infected CEFs was independent of virus replication and occurred at an early stage following in vitro infection.
Tham
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KM
Virology Section, Central Animal Health Laboratory, Upper Hutt, New Zealand.
Moon
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CD
D000818:Animals; D017209:Apoptosis / Q000235:genetics / Q000502:physiology*; D001483:Base Sequence; D018175:Birnaviridae Infections / Q000503:physiopathology / Q000662:veterinary*; D002478:Cells, Cultured; D002642:Chick Embryo; D002522:Chlorocebus aethiops; D004249:DNA Damage; D005347:Fibroblasts; D007243:Infectious bursal disease virus / Q000502:physiology; D008969:Molecular Sequence Data; D014709:Vero Cells; D014779:Virus Replication