Metabolism of illudin S, a toxic substance of Lampteromyces japonicus: urinary excretion of mercapturic acids in rat.
1. The urinary excretion of the mercapturic acids of illudin S after oral administration to rat has been studied. 2. From lc-ms/ms analysis of methanolic extracts of lyophilized rat urine, stereo-isomeric mercapturic acids were detected. 3. The mercapturic acids excreted 3 days following administration amounted to approximately 0.39-0.73% of the administered dose. 4. In vitro glutathione conjugation of illudin S by subcellular fractions was also examined. 5. No significant increases in the formation of glutathione adducts were observed in any subcellular fractions examined.
Tanaka
K
K
National Research Institute of Police Science, Tokyo, Japan.
Inoue
T
T
Tezuka
Y
Y
Kikuchi
T
T
D000111:Acetylcysteine / Q000378:metabolism / Q000652:urine*; D000363:Agaricales; D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000378:metabolism / Q000652:urine*; D005978:Glutathione / Q000378:metabolism; D008297:Male; D000081223:Polycyclic Sesquiterpenes; D051381:Rats; D017208:Rats, Wistar; D012717:Sesquiterpenes / Q000378:metabolism / Q000652:urine; D013347:Subcellular Fractions / Q000378:metabolism
D000903:Antibiotics, Antineoplastic; D000081223:Polycyclic Sesquiterpenes; D012717:Sesquiterpenes; C008105:illudin S; D005978:Glutathione; D000111:Acetylcysteine