title: The viability of cryopreserved onlay cranial bone allografts: a comparative experimental study versus fresh autografts.
abstract: It is well known that calvarial bone autografts are the bone grafts that are the least reabsorbent and have the best long-term evolution in craniofacial surgery. However, they do have certain limitations: (1) reabsorption results in repeated surgery and the need for new donor areas, (2) a limited amount of autogenous cranial bone is available (due to avoiding areas close to cranial sutures and venous sinuses, and because the temporal bone is very fragile and the cranium has not fully developed in children), and (3) graft extraction increases surgical time and morbidity. Because of this, we present an alternative to calvarial bone autografts: cryopreserved allografts. This paper is an experimental prospective study carried out on sheep with the following goals: (1) to assess the behavior of calvarial onlay bone grafts cryopreserved at -80 degrees C, using fresh autografts implanted under the same conditions as controls; (2) to compare reabsorption percentages statistically over time; and (3) to study qualitatively any histological variations. The results obtained are (1) more reabsorption of allografts when compared to autografts (at 90 days, 21.97% versus 20.21% of grafted volume), although this difference is not statistically significant; (2) a reduction in height in all onlay grafts as a consequence of the loss of the diplöe; (3) the absence of any type of inflammation caused by a reaction to cryopreserved allografts; and (4) bone substitution performed using frozen allografts is histologically similar to that using fresh autografts.
authors.affiliation: Department of Plastic, Reconstructive and Aesthetic Surgery, Clínica Universitaria de Navarra, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Sanz
authors.forename: J
authors.initials: J
authors.lastname: Elejabeitia
authors.forename: A
authors.initials: A
authors.lastname: Bazán
authors.forename: E
authors.initials: E
authors.lastname: García-Tutor
authors.forename: V
authors.initials: V
authors.lastname: Paloma
mesh_terms: D015925:Cryopreservation; D006801:Humans; D012886:Skull; D014021:Tissue Preservation