The influence of 50-Hz magnetic fields on cytoplasmic Ca2+ oscillations in human leukemia T-cells.
We have studied the effects of 50-Hz 100-microT rms magnetic fields on intracellular Ca2+ concentration in the Jurkat T lymphocyte variant E6.1 using fluorescent probes Indo-1 and Fura-2. We found, however, that the pattern of intracellular Ca2+ fluctuations also depended on the agent used for cell attachment, in our case the polypeptide poly-L-lysine. In order to isolate possible effects of magnetic field exposure from those of poly-L-lysine, the action of polypeptide on cytosolic Ca2+ was studied as well. It was found that a 10(-7)% concentration of polypeptide triggered prolonged Ca2+ spiking. Higher (10(-4)%) concentrations induced rapid increases in intracellular Ca2+ followed by high, unstable Ca2+ levels. The response of these cells to the monoclonal antibody anti-CD3 was also inhomogeneous, similar to one caused by poly-L-lysine. The effect of magnetic field exposure was studied on cells initially exhibiting (1) non-oscillating, low Ca2+ concentration and (2) prolonged Ca2+ concentration oscillations. In case (1) the result was negative. In case (2), statistically significant changes were found: the oscillation amplitude was reduced on average by 30%, and the frequency composition was shifted towards higher frequencies.
Galvanovskis
J
J
Department of Medical Biophysics, University of Gothenburg, Sweden.
Sandblom
J
J
Bergqvist
B
B
Galt
S
S
Hamnerius
Y
Y
D002118:Calcium / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism*; D004574:Electromagnetic Fields; D006801:Humans; D007938:Leukemia / Q000378:metabolism / Q000473:pathology; D011107:Polylysine; D013601:T-Lymphocytes / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D011107:Polylysine; D002118:Calcium