title: Molecular scanning of candidate mitochondrial tRNA genes in type 2 (non-insulin dependent) diabetes mellitus.
abstract: Mitochondrial DNA (mtDNA) gene defects may play a role in the development of non-insulin dependent diabetes mellitus (NIDDM). In order to search for potentially diabetogenic mtDNA defects we have applied the technique of single stranded conformational polymorphism (SSCP) analysis to 124 patients with a history of NIDDM and 40 non-diabetic controls. No new heteroplasmic mutations were detected. However, a variety of homoplasmic variants were found in patients with NIDDM; some of these merit further investigation.
authors.affiliation: Department of Medicine, University Hospital of Wales, Heath Park, Cardiff, UK.
authors.forename: A W
authors.initials: AW
authors.lastname: Thomas
authors.forename: A
authors.initials: A
authors.lastname: Edwards
authors.forename: E J
authors.initials: EJ
authors.lastname: Sherratt
authors.forename: A
authors.initials: A
authors.lastname: Majid
authors.forename: J
authors.initials: J
authors.lastname: Gagg
authors.forename: J C
authors.initials: JC
authors.lastname: Alcolado
mesh_terms: D001483:Base Sequence; D004272:DNA, Mitochondrial / Q000235:genetics*; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics*; D006801:Humans; D008928:Mitochondria; D008969:Molecular Sequence Data; D017354:Point Mutation; D018807:Polymorphism, Single-Stranded Conformational; D012313:RNA / Q000235:genetics*; D000077278:RNA, Mitochondrial; D012343:RNA, Transfer / Q000235:genetics*; D012016:Reference Values; D012091:Repetitive Sequences, Nucleic Acid; D017384:Sequence Deletion
chemical_list: D004272:DNA, Mitochondrial; D000077278:RNA, Mitochondrial; D012313:RNA; D012343:RNA, Transfer