title: Interleukin-1 beta stimulates glucose uptake of human peritoneal mesothelial cells in vitro.
abstract: To investigate whether the glucose uptake (GU) of human peritoneal mesothelial cells (HPMC) is mediated by glucose transporters and whether this uptake is influenced by interleukin 1-beta (IL-1 beta), we measured 2-deoxy-(3H)-GU of HPMC in vitro, after exposing the cells for different times (two and 12 hours) to increasing concentrations (0.1, 1.0, and 2.0 ng/mL) of IL-1 beta. To exclude a noncarrier-mediated transport, GU was also tested in the presence of cytochalasin B. All experiments were performed in triplicate in the cells of two donors. Cytochalasin B inhibits GU of HPMC almost completely. GU of HPMC is not stimulated by insulin. GU is stimulated by IL-1 beta in a dose-dependent manner. These data indicate a GU of HPMC, which is mediated by a glucose transporter and stimulated by IL-1 beta. The increased uptake of glucose from the dialysate in patients with peritonitis may be mediated by a (cytokine-induced) increased activity of HPMC glucose transporters.
authors.affiliation: Department of Nephrology, Hannover Medical School, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Kruse
authors.forename: A
authors.initials: A
authors.lastname: Mahiout
authors.forename: V
authors.initials: V
authors.lastname: Kliem
authors.forename: P
authors.initials: P
authors.lastname: Kurz
authors.forename: K M
authors.initials: KM
authors.lastname: Koch
authors.forename: R
authors.initials: R
authors.lastname: Brunkhorst
mesh_terms: D001786:Blood Glucose / Q000378:metabolism*; D003571:Cytochalasin B / Q000494:pharmacology; D003847:Deoxyglucose / Q000378:metabolism; D015314:Dialysis Solutions / Q000493:pharmacokinetics; D004305:Dose-Response Relationship, Drug; D004847:Epithelial Cells; D006801:Humans; D066298:In Vitro Techniques; D007375:Interleukin-1 / Q000494:pharmacology*; D009004:Monosaccharide Transport Proteins / Q000502:physiology; D010537:Peritoneum / Q000166:cytology*; D010538:Peritonitis / Q000473:pathology
chemical_list: D001786:Blood Glucose; D015314:Dialysis Solutions; D007375:Interleukin-1; D009004:Monosaccharide Transport Proteins; D003571:Cytochalasin B; D003847:Deoxyglucose