Partial characterization of an immortalized human trophoblast cell-line, TCL-1, which possesses a CSF-1 autocrine loop.
Many previous studies in both mouse and human placenta have implicated a role for colony stimulating factor-1 (CSF-1) in the regulation of placental development. In this study we have examined CSF-1 production by an immortalized cell line (TCL-1) derived from the choriodecidua, transfected with a retrovirus gene coding for the large-T antigen. TCL-1 cells were uniformly positive by immunocytochemistry for the composite sub-units of human chorionic g gonadotrophin (hCG) but were negative for markers of other cell types localized at the fetal-maternal interface. Gelatinase enzymes were secreted by TCL-1 cells cultured on extracellular matrix in a manner indicative of extra-villous trophoblast. Dot-blot immunoassays and ELISA indicated that CSF-1 was secreted by TCL-1 cells, at levels comparable to primary trophoblast cells and BeWo choriocarcinoma (trophoblast tumour) cells. Reverse transcriptase-polymerase chain reaction analysis confirmed the presence in TCL-1 cells of CSF-1 receptor mRNA (c-fms gene product), indicating that the components of a potential autocrine loop were present in these cells. Proliferation of TCL-1 cells was not affected by the addition of exogenous CSF-1 but was elevated in response to treatment with a CSF-1 neutralizing antibody. The immortalized cell line, TCL-1, provides a potential model in which to investigate regulation of growth and differentiation of trophoblast cells in vitro.
Lewis
M P
MP
Institute of Obstetrics & Gynaecology, Royal Postgraduate Medical School, Hammersmith Hospital, London, UK.
Clements
M
M
Takeda
S
S
Kirby
P L
PL
Seki
H
H
Lonsdale
L B
LB
Sullivan
M H
MH
Elder
M G
MG
White
J O
JO
D001483:Base Sequence; D015415:Biomarkers; D002455:Cell Division; D002460:Cell Line; D002823:Chorion / Q000166:cytology / Q000378:metabolism*; D002999:Clone Cells; D017364:Collagenases / Q000378:metabolism; D017077:Culture Media, Conditioned; D003656:Decidua / Q000166:cytology / Q000378:metabolism*; D010450:Endopeptidases / Q000032:analysis; D005260:Female; D018093:Gelatinases / Q000378:metabolism; D006801:Humans; D016173:Macrophage Colony-Stimulating Factor / Q000032:analysis / Q000096:biosynthesis*; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9; D008666:Metalloendopeptidases / Q000378:metabolism; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis; D016186:Receptor, Macrophage Colony-Stimulating Factor / Q000235:genetics; D014162:Transfection; D014327:Trophoblasts / Q000166:cytology / Q000378:metabolism*
D015415:Biomarkers; D017077:Culture Media, Conditioned; D012333:RNA, Messenger; D016173:Macrophage Colony-Stimulating Factor; D016186:Receptor, Macrophage Colony-Stimulating Factor; D010450:Endopeptidases; D017364:Collagenases; D018093:Gelatinases; D008666:Metalloendopeptidases; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9