title: Influence of surface charges on cell adhesion: difference between static and dynamic conditions.
abstract: We tested the hypothesis that nonspecific repulsion, as a result of electrostatic forces and (or) steric stabilization effects, impaired adhesion more efficiently under dynamic than under static conditions. Cells from the human monocytic line THP1 were plated on a glass surface. Spherical particles bearing monoclonal antibodies specific for antigens expressed by THP1 cells (CD11b, CD18, CD35, CD64) were then added and adhesion was quantified. The effect of neuraminidase treatment of THP1 cells was also studied. Adhesion was then measured in a flow chamber under low shear flow (wall shear rate was 11 or 22 s-1), allowing a quantitative determination of cell adhesion frequency. The following conclusions were obtained: (i) under static conditions, neuraminidase treatment had little effect on adhesion (only CD18-mediated interaction was significantly increased at 4 degrees C after enzyme treatment); (ii) under dynamic conditions, neuraminidase treatment significantly increased binding; (iii) surprisingly, there was no clear relationship between the length of adhesion molecules involved in the interaction and binding efficiency; and (iv) such parameters as cell shape and topographical distribution of adhesion molecules may strongly influence adhesion under flow. It is concluded that a dynamic reorganization of the pericellular matrix following intercellular contact may play an important role in regulating adhesion.
authors.affiliation: Unité INSERM 387, Hôpital de Sainte-Marguerite, Marseille, France.
authors.forename: S
authors.initials: S
authors.lastname: Sabri
authors.forename: A
authors.initials: A
authors.lastname: Pierres
authors.forename: A M
authors.initials: AM
authors.lastname: Benoliel
authors.forename: P
authors.initials: P
authors.lastname: Bongrand
mesh_terms: D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology*; D002460:Cell Line; D048429:Cell Size; D004560:Electricity; D006801:Humans; D008954:Models, Biological; D009038:Motion; D009439:Neuraminidase / Q000494:pharmacology; D013499:Surface Properties
chemical_list: D009439:Neuraminidase