Growth hormone did not enhance mucosal hyperplasia after small-bowel resection.
BACKGROUND
Insulin-like growth factor (IGF)-I enhances the mucosal hyperplasia that normally occurs after massive small-bowel resection. The present studies examined whether growth hormone (GH) infusion increases serum IGF-I levels and, thereby, enhances mucosal hyperplasia after small-bowel resection.


METHODS
Male Sprague-Dawley rats weighing approximately 140 g were subjected to 80% jejunoileal resection or sham operation (ileal transection). A mini-osmotic pump was then inserted under the skin immediately after surgery to deliver either vehicle or 3mg/kg/day GH. All animals were killed 7 days postoperatively, and the remaining intestine was removed and divided at the anastomotic site.


RESULTS
Food intake did not differ between control and GH-infused groups. Resected rats infused with GH grew faster than resected rats infused with vehicle. However, GH did not stimulate the weight gain of sham-operated rats. In both duodenojejunum and ileum, GH infusion did not stimulate hyperplasia beyond that which normally occurs after small-bowel resection, nor did it increase mucosal mass in sham-operated rats. GH infusion did not alter serum IGF-I or IGF-binding protein (IGFBP) levels in either resected or sham-operated rats. GH infusion resulted in increased sucrase and maltase activities in the ileal mucosa of resected rats.


CONCLUSIONS
The present results suggest that a pharmacologic dose of GH directly stimulates growth of resected animals and ileal sucrase and maltase activities without increasing serum IGF-I levels or increasing absorptive surface area in the small intestine.
Park
J H
JH
Dept. of Food and Nutrition, Hallym University, Chunchon, Korea.
Vanderhoof
J A
JA
D000818:Animals; D013006:Growth Hormone / Q000494:pharmacology*; D006965:Hyperplasia; D018969:Insulin-Like Growth Factor Binding Proteins / Q000032:analysis; D007334:Insulin-Like Growth Factor I / Q000032:analysis; D007413:Intestinal Mucosa / Q000187:drug effects* / Q000473:pathology*; D007421:Intestine, Small / Q000187:drug effects / Q000601:surgery*; D007583:Jejunum / Q000601:surgery; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
D018969:Insulin-Like Growth Factor Binding Proteins; D007334:Insulin-Like Growth Factor I; D013006:Growth Hormone