title: Identification of genetic factors for Alachlor tolerance in maize by molecular markers analysis.
abstract: Genetic factors controlling tolerance to the herbicide Alachlor in maize were localised by means of two different strategies. In the first approach, backcross (BC) plants, derived from pollen which had been subjected to selective pressure for resistance to the herbicide, were analysed for segregation distortion at 47 RFLP loci and compared to BC plants obtained from non-selected pollen. Preferential transmission of five chromosomal regions where putative QTLs (Quantitative Trait Loci) are localised was revealed in the BC plants from selected pollen. A second approach was based on a classical linkage analysis for segregation of the same set of RFLPs and factors controlling the trait, in a BC population of 210 individuals, by means of regression analysis. This study detected seven significant loci in four genomic regions. Overall, two loci revealed both segregation distortion and association with the expression of the trait, indicating linkage to genes expressed in both gametophytic and sporophytic phase. Three chromosomal regions appeared to carry factors involved in plant tolerance to Alachlor which are not expressed in pollen. Conversely, three loci were linked to factors selectable in pollen, but did not reveal significant association with tolerance in the plant in the segregating populations.
authors.affiliation: Department of Genetics and Microbiology, University of Milan, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Sari-Gorla
authors.forename: L
authors.initials: L
authors.lastname: Rampoldi
authors.forename: G
authors.initials: G
authors.lastname: Binelli
authors.forename: C
authors.initials: C
authors.lastname: Frova
authors.forename: M E
authors.initials: ME
authors.lastname: Pè
mesh_terms: D000081:Acetamides / Q000494:pharmacology*; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D005787:Gene Frequency; D017343:Genes, Plant / Q000235:genetics*; D008040:Genetic Linkage; D005819:Genetic Markers; D006540:Herbicides / Q000494:pharmacology*; D012150:Polymorphism, Restriction Fragment Length; D003313:Zea mays / Q000187:drug effects* / Q000235:genetics
chemical_list: D000081:Acetamides; D005819:Genetic Markers; D006540:Herbicides; C000188:alachlor