Mutations of the gyrA gene of clinical isolates of Salmonella typhimurium and three other Salmonella species leading to decreased susceptibilities to 4-quinolone drugs.
The region of the gyrA gene encoding nucleotides 72 to 557 in ten Salmonella typhimurium clinical isolates received from Vellore, India and six British clinical isolates of a number of Salmonella species has been sequenced. Those exhibiting a decreased susceptibility to 4-quinolone drugs (MICs of ciprofloxacin ranging from 0.128 mg/L to 1.0 mg/L) have been shown to possess a number of different mutations in this region of the gene (Asp-87 to Gly, Asp-87 to Asn and Ser-83 to Phe). The mutations Asp-87 to Gly and Ser-83 to Phe/Asp-87 to Asn have yet to be reported occurring in S. typhimurium.
Brown
J C
JC
Department of Medical Microbiology, Medical School, University of Edinburgh, UK.
Thomson
C J
CJ
Amyes
S G
SG
D000595:Amino Acid Sequence; D000890:Anti-Infective Agents / Q000494:pharmacology*; D001483:Base Sequence; D002939:Ciprofloxacin / Q000494:pharmacology*; D004352:Drug Resistance, Microbial; D005798:Genes, Bacterial; D007194:India; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D017354:Point Mutation; D012475:Salmonella / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D012486:Salmonella typhimurium / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D006113:United Kingdom
D000890:Anti-Infective Agents; D002939:Ciprofloxacin