Spectral analysis of blood pressure and heart rate, catecholamine and neuropeptide Y plasma levels in a new model of neurogenic orthostatic hypotension in dog.
The aim of the study was to compare changes in blood pressure (BP) and heart rate (HR) variability, catecholamine and neuropeptide Y (NPY) plasma levels induced by passive head-up tilt in normal and sino-aortic denervated (SAD) chloralose-anaesthetized dogs. In controls, 80 degrees head-up tilt test failed to change BP and increased HR. Plasma noradrenaline and NPY levels (but not adrenaline) significantly rose. In SAD dogs, head-up tilt test induced a marked and reproducible decrease in BP without any change in HR or noradrenaline and NPY plasma levels. In SAD dogs, spectral analysis in supine position was characterized by reduced variability in the high frequency (HF) band of the HR spectrum without changes in low frequency (LF) bands of both HR and systolic blood pressure (SBP). Head-up tilt test increased the LF component of SBP variability and decreased the HF component of HR variability in controls but failed to modify HR and BP variabilities in SAD dogs. In conclusion, sino-aortic denervation in dogs elicits a reproducible postural fall in BP with impaired adaptation of sympathetic nervous system activity. This model may be of value in evaluating the pharmacological effects of drugs for the management of orthostatic hypotension.
Verwaerde
P
P
Laboratoire de Pharmacologie Médicale et Clinique, INSERM U317, Faculty of Medicine, Toulouse, France.
Senard
J M
JM
Mazerolles
M
M
Tran
M A
MA
Damase-Michel
C
C
Montastruc
J L
JL
Montastruc
P
P
D000222:Adaptation, Physiological; D000319:Adrenergic beta-Antagonists / Q000494:pharmacology; D000818:Animals; D001794:Blood Pressure / Q000187:drug effects / Q000502:physiology*; D002395:Catecholamines / Q000097:blood*; D002698:Chloralose; D004285:Dogs; D006339:Heart Rate / Q000187:drug effects / Q000502:physiology*; D007024:Hypotension, Orthostatic / Q000097:blood / Q000503:physiopathology*; D008297:Male; D018727:Muscarinic Antagonists / Q000494:pharmacology; D009478:Neuropeptide Y / Q000097:blood*; D012016:Reference Values; D012121:Respiration, Artificial; D017550:Spectroscopy, Fourier Transform Infrared; D018667:Tilt-Table Test
D000319:Adrenergic beta-Antagonists; D002395:Catecholamines; D018727:Muscarinic Antagonists; D009478:Neuropeptide Y; D002698:Chloralose