[Isolation of Y-specific probes in cattle applying genetically representational difference analysis].
Genetically directed representational difference analysis (GDRDA) allows to isolate DNA fragments that differ between complex genomes. This approach was applied in the present work to isolate Y-specific bovine sequences. Of eight isolated and cloned fragments, two proved to be Y-specific.
Wigger
G
G
Institut für Nutztierwissenschaften, ETH Zürich.
Fries
R
R
Stranzinger
G
G
D000818:Animals; D001483:Base Sequence; D002417:Cattle / Q000235:genetics*; D003001:Cloning, Molecular; D017931:DNA Primers; D015342:DNA Probes; D005260:Female; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary; D012727:Sex Characteristics; D014998:Y Chromosome
D017931:DNA Primers; D015342:DNA Probes
Die Isolierung Y-spezifischer Sonden beim Rind mittels genetischer Repräsentations-Differenz-Analyse.