title: Monocyte activation on titanium-sputtered polystyrene surfaces in vitro: the effect of culture conditions on interleukin-1 release.
abstract: The release of interleukin-1 alpha (IL-1 alpha) by human peripheral blood monocytes cultured for 24 and 48 h on polystyrene (PS) and titanium-sputtered polystyrene (Ti) was evaluated. Magnetron sputtering of the PS surfaces resulted in a formation of a 50-nm-thick coat, consisting of an outer layer of TiO2. Monocytes released IL-1 alpha without the addition of exogenous stimuli. A doubling of the culture time from 24 to 48 h did not have a major effect on the amount of IL-1 alpha released. The IL-1 alpha levels were increased by addition of lipopolysaccharide (LPS). High concentrations of PS particles (1 and 3 microns diameter) were equally effective stimuli for IL-1 alpha release as LPS. Preadsorption of fibronectin to culture plates augmented LPS-stimulated IL-1 alpha secretion, whereas preadsorbed fibrinogen had an inhibitory effect. Our observation indicate a direct activation of monocytes by PS and Ti, resulting in IL-1 alpha secretion, which is modified by protein adsorption and exogenous stimuli.
authors.affiliation: Institute of Anatomy and Cell Biology, Gothenburg University, Sweden.
authors.forename: C
authors.initials: C
authors.lastname: Gretzer
authors.forename: A S
authors.initials: AS
authors.lastname: Eriksson
authors.forename: B
authors.initials: B
authors.lastname: Alldén
authors.forename: L E
authors.initials: LE
authors.lastname: Ericson
authors.forename: P
authors.initials: P
authors.lastname: Thomsen
mesh_terms: D000327:Adsorption; D001672:Biocompatible Materials; D002478:Cells, Cultured; D005340:Fibrinogen; D005353:Fibronectins; D006801:Humans; D066298:In Vitro Techniques; D007375:Interleukin-1 / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008422:Materials Testing; D008854:Microscopy, Electron; D009000:Monocytes / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D011137:Polystyrenes; D019736:Prostheses and Implants; D013499:Surface Properties; D014025:Titanium; D014945:Wound Healing / Q000502:physiology
chemical_list: D001672:Biocompatible Materials; D005353:Fibronectins; D007375:Interleukin-1; D008070:Lipopolysaccharides; D011137:Polystyrenes; D005340:Fibrinogen; D014025:Titanium