title: Cytopathology and release of an RNA virus from a strain of Trichomonas vaginalis.
abstract: A strain of Trichomonas vaginalis infected with a double-stranded RNA virus showed pronounced cytopathology in the form of giant syncytia generated by the recruitment of single cells. The giant cells ultimately lysed, releasing virus into the culture medium. In the infected cells, clusters of electron-dense particles resembling viral structures were found in the cytoplasm. In addition, distinctive inclusions composed of similar particles were present in the nuclei of some cells. Double-stranded viral RNA of 5.5 kbp was demonstrated in both cytoplasmic and nuclear fractions from these cells. Viral particles collected from the cell-free culture supernatant were of the same shape and size as the RNA virus isolated from a strain of T. vaginalis described previously (Wang & Wang, Journal of Biological Chemistry, 260: 3697-3702, 1985; Wang & Wang, Proceedings of the National Academy of Sciences of the U.S.A. 83: 7956-7960, 1986) which does not show this cytopathology.
authors.affiliation: Department of Biochemistry, East Tennessee State University, Johnson City 37614, USA.
authors.forename: W S
authors.initials: WS
authors.lastname: Champney
authors.forename: S K
authors.initials: SK
authors.lastname: Curtis
authors.forename: R
authors.initials: R
authors.lastname: Samuels
mesh_terms: D000818:Animals; D003588:Cytopathogenic Effect, Viral; D007181:Inclusion Bodies, Viral / Q000648:ultrastructure; D008854:Microscopy, Electron; D012328:RNA Viruses / Q000302:isolation & purification* / Q000472:pathogenicity / Q000648:ultrastructure; D012367:RNA, Viral / Q000302:isolation & purification; D014246:Trichomonas vaginalis / Q000648:ultrastructure / Q000821:virology*
chemical_list: D012367:RNA, Viral