[Ras gene analysis in mammary tumors of dogs by means of PCR-SSCP and direct genomic analysis].
The oncogenic capacities of RAS family genes (Ha-ras, Ki-ras, and N-ras) are usually activated by point mutations in the conserved regions (codons 12, 13, and 61), resulting in single amino acid substitution in the specific proteins (p21). In order to verify the involvement of RAS genes in dog mammary tumors we analyzed the genomic DNA from 20 mammary tumors of dog by means of the Polymerase Chain Reaction-Single Strand Conformation Polymorphism (PCR-SSCP) method and the direct genomic sequencing. The absence of point mutations in the "hot spots" of RAS genes suggests a lack or a low frequency of such a pattern of RAS genes activation in dog mammary tumors. The results are also in agreement to what reported in human mammary tumors. However, the presence of genetic alterations in other functional areas of the RAS genes or other mechanisms of activations cannot be ruled out.
Castagnaro
M
M
Dipartimento di Patologia Animale, Università degli Studi, Torino.
D000818:Animals; D001483:Base Sequence; D004273:DNA, Neoplasm / Q000235:genetics; D004285:Dogs; D005260:Female; D011905:Genes, ras / Q000235:genetics*; D008325:Mammary Neoplasms, Experimental / Q000235:genetics*; D008969:Molecular Sequence Data; D017354:Point Mutation; D016133:Polymerase Chain Reaction / Q000379:methods; D018807:Polymorphism, Single-Stranded Conformational
D004273:DNA, Neoplasm
Analisi dei geni RAS nelle neoplasie mammarie del cane mediante la tecnica PCR-SSCP e il sequenziamento genomico diretto.