Inhibitory effects of selenium, vitamin A and butylated hydroxytoluene on in vitro growth of human tongue cancer cells.
The effects of vitamin A, selenium and butylated hydroxytoluene (BHT) on the growth of a human tongue cancer cell line were examined in monolayer cell cultures. A colony-forming assay showed a 50% reduction in the survival rate of the cell line at a concentration of 2.6 micrograms/ml selenium, 60 micrograms/ml vitamin A and/or 38 micrograms/ml BHT. Relatively low concentrations of selenium markedly inhibited glucose consumption. Flow cytometric analysis with both fluorescein-isothiocyanate-labelled bromodeoxyuridine monoclonal antibody and propidium iodide demonstrated an increase in S-phase fractions 1 day after the addition of selenium, increased G0/G1 phase fractions in the presence of vitamin A and increased G2-M phase fractions when BHT was added. These results suggest that these compounds inhibit DNA synthesis of in vitro human tongue cancer cells by different mechanisms.
Inoue
I
I
Department of Otolaryngology, Osaka Medical College, Japan.
Yamamoto
Y
Y
Takasaki
T
T
Takahashi
H
H
D002084:Butylated Hydroxytoluene / Q000494:pharmacology*; D018929:Cell Culture Techniques; D018890:Chemoprevention; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D006801:Humans; D012643:Selenium / Q000494:pharmacology*; D014059:Tongue / Q000473:pathology*; D014062:Tongue Neoplasms / Q000473:pathology*; D014801:Vitamin A / Q000494:pharmacology*
D014801:Vitamin A; D002084:Butylated Hydroxytoluene; D012643:Selenium