An antibody specific to N-terminal amino acid sequence of human maternal serum cystine aminopeptidase and its applications.
An antibody directed against a synthetic peptide corresponding to the N-terminal sequence of human cystine aminopeptidase (CAP) present in maternal serum was prepared. Although the antibody did not immunoprecipitate the activity of CAP, it was useful for purification and immunoblot analysis of CAP protein. An antipeptide antibody-conjugated Sepharose 4B column was very effective in purifying a single CAP protein from partially purified enzyme preparation, and Western blotting confirmed the binding of the antibody to CAP protein.
Watanabe
Y
Y
Department of Clinical Biochemistry, Hokkaido Institute of Pharmaceutical Sciences, Japan.
Iwaki-Egawa
S
S
Yokosawa
J
J
Mizukoshi
H
H
Fujimoto
Y
Y
D000595:Amino Acid Sequence; D000906:Antibodies / Q000737:chemistry / Q000276:immunology; D000918:Antibody Specificity; D002846:Chromatography, Affinity; D010122:Cystinyl Aminopeptidase / Q000097:blood* / Q000276:immunology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000276:immunology; D011247:Pregnancy / Q000097:blood*
D000906:Antibodies; D010446:Peptide Fragments; D010122:Cystinyl Aminopeptidase