Indirect examination of exocrine pancreatic function in vivo by determination of chymotrypsin activity in animal intestine using a synthetic substrate.
Using the substrate N-acetyl-L-tyrosyl-p-aminobenzoic acid, we determined chymotrypsin activity in the small intestine of calf, pig, and poultry. Orally administered N-acetyl-L-tyrosyl-p-aminobenzoic acid is enzymatically cleaved in vivo, and the released p-aminobenzoic acid is determined by HPLC. We found that the p-aminobenzoic acid concentration in plasma and urine was significantly influenced by the feeding of soya flour. After soybean flour feeding, the p-aminobenzoic acid concentration significantly increased in the plasma of calves and hens, in contrast to pigs, where the p-aminobenzoic acid concentration significantly decreased. This shows that the oral administration of N-acetyl-L-tyrosyl-p-aminobenzoic acid with subsequent determination of p-aminobenzoic acid is suitable for the estimation of exocrine pancreatic function and for determination of changes in intestinal proteolytic activity caused by antinutritive substances.
Kastel
R
R
Department of Nutrition, Dietetics and Forage, University of Veterinary Medicine, Kosice, Slovak Republic.
Rosival
I
I
Bartik
M
M
Blahovec
J
J
D010129:4-Aminobenzoic Acid / Q000032:analysis* / Q000097:blood / Q000378:metabolism / Q000652:urine; D000821:Animal Feed; D000818:Animals; D002417:Cattle; D002645:Chickens; D002851:Chromatography, High Pressure Liquid; D002918:Chymotrypsin / Q000378:metabolism*; D005243:Feces / Q000737:chemistry; D005260:Female; D007422:Intestines / Q000201:enzymology*; D008297:Male; D010179:Pancreas / Q000201:enzymology*; D013552:Swine; D062366:para-Aminobenzoates
D062366:para-Aminobenzoates; C020187:4-((N-Acetyl-L-tyrosyl)amino)benzoic acid; D002918:Chymotrypsin; D010129:4-Aminobenzoic Acid