title: Use of a single slide trichrome-stained concentrate for the detection of intestinal parasites. Stained concentration procedure for ova and parasites.
abstract: The detection and identification of intestinal parasites was studied using a single slide of a trichrome-stained concentrate made from a cupric-polyvinyl alcohol (PVA)-preserved specimen. When saline was used in place of formalin in the concentration procedure, it was possible to adequately stain the concentrate with a modified-trichrome stain. This was compared to a more traditional two-slide ova and parasite (O&P) procedure. Of the 1,000 specimens evaluated, a total of 261 parasites were detected using the single slide, modified trichrome-stained concentrate compared to 249 from the formalin/Hemo-De wet mount and 226 from the unconcentrated trichrome stain. The detection rate of the single slide method was comparable to the combined detection rate of the two-slide method. The saline/Hemo-De concentration procedure has the advantage of using nontoxic saline in place of formaldehyde. This new method is more cost-effective because it requires the processing and evaluation of only one slide.
authors.affiliation: Department of Pathology, University of Utah Medical School, Salt Lake City, USA.
authors.forename: D C
authors.initials: DC
authors.lastname: Hale
authors.forename: K
authors.initials: K
authors.lastname: Carroll
authors.forename: J R
authors.initials: JR
authors.lastname: Kucera
authors.forename: W E
authors.initials: WE
authors.lastname: Aldeen
mesh_terms: D000818:Animals; D002498:Centrifugation; D056890:Eukaryota / Q000469:parasitology*; D005069:Evaluation Studies as Topic; D005243:Feces / Q000469:parasitology; D006376:Helminths / Q000469:parasitology*; D006801:Humans; D007411:Intestinal Diseases, Parasitic / Q000175:diagnosis* / Q000469:parasitology; D010063:Ovum / Q000166:cytology; D013048:Specimen Handling / Q000379:methods*; D013194:Staining and Labeling / Q000379:methods*