Simultaneous quantitative determination of metronidazole and nalidixic acid in tablets by difference spectroscopy.
A difference spectrophotometric procedure has been developed for simultaneous determination of metronidazole (MDZ) and nalidixic acid (NA) in tablets. The method comprised the measurement of the absorbance of a solution of the tablet extract in 0.1 M NaOH relative to that of an equimolar solution in 0.1 M HCl at 292 nm for NA and 325 nm for MDZ. The presence of identical isosbestic points for pure drug solutions and tablet extracts indicated the non-interference of excipients in the absorption at these wavelengths. Compliance with Beer's law was observed in the concentration ranges 5-25 micrograms ml(-1) for MDZ and 15-35 micrograms ml(-1) for NA these wavelengths.
Parimoo
P
P
Department of Pharmacy, Birla Institute of Technology and Science, Rajasthan, India.
Prasad
C V
CV
Vineeth
R
R
D000890:Anti-Infective Agents / Q000032:analysis*; D000994:Antitrichomonal Agents / Q000032:analysis*; D004338:Drug Combinations; D008795:Metronidazole / Q000032:analysis*; D009268:Nalidixic Acid / Q000032:analysis*; D013053:Spectrophotometry; D013607:Tablets
D000890:Anti-Infective Agents; D000994:Antitrichomonal Agents; D004338:Drug Combinations; D013607:Tablets; D008795:Metronidazole; D009268:Nalidixic Acid