Retrotransposon BARE-1 is a major, dispersed component of the barley (Hordeum vulgare L.) genome.
The barley BARE-1 is a transcribed, copia-like retroelement with well-conserved functional domains, an active promoter, and a copy number of at least 3 x 10(4). We examined its chromosomal localization by in situ hybridization. The long terminal repeat (LTR) probe displayed a uniform hybridization pattern over the whole of all chromosomes, excepting paracentromeric regions, telomeres, and nucleolar organizer (NOR) regions. The integrase probe showed a similar pattern. The 5'-untranslated leader (UTL) probe, expected to be the most rapidly evolving component, labeled chromosomes in a dispersed and non-uniform manner, concentrated in the distal regions, possibly indicating a targe site preference.
Suoniemi
A
A
Institute of Biotechnology, University of Helsinki, Finland.
Anamthawat-Jónsson
K
K
Arna
T
T
Schulman
A H
AH
D001483:Base Sequence; D002874:Chromosome Mapping; D017931:DNA Primers; D018745:Genome, Plant; D001467:Hordeum / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D012091:Repetitive Sequences, Nucleic Acid; D018626:Retroelements
D017931:DNA Primers; D018626:Retroelements