Simultaneous high-performance liquid chromatographic determination of caffeine and theophylline for routine drug monitoring in human plasma.
An HPLC method for the simultaneous determination of both caffeine and theophylline in human plasma is described, using a reversed-phase chromatography column, heated by a thermostatic oven at 35 degrees C, with UV detection and isocratic elution. The linearity and reproducibility of the method are verified. For the two drugs, the limit of detection is 0.1 microgram ml-1. This analytical method is rapid and reliable and allows routine controls of therapeutic levels of theophylline and caffeine, especially in premature infants where the volume of plasma samples is very small.
Schreiber-Deturmeny
E
E
Laboratoire de Pharmacologie Médicale et Clinique, Faculté de Médecine et CHU Timone, Marseille, France.
Bruguerolle
B
B
D002110:Caffeine / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet; D013806:Theophylline / Q000097:blood*
D002110:Caffeine; D013806:Theophylline