The genetics of the repair of 5-azacytidine-mediated DNA damage in the fission yeast Schizosaccharomyces pombe.
We have recently demonstrated that Schizosaccharomyces pombe cells treated with the nucleoside analogue 5-azacytidine (5-azaC) require previously characterised G2 checkpoint mechanisms for survival. Here we present a survey of known DNA repair mutations which defines those genes required for survival in the presence of 5-azaC. Using a combination of single-mutant and epistasis analyses we find that the excision, mismatch and recombinational repair pathways are all required in some degree for the repair of 5-azaC-mediated DNA damage. There are distinct differences in the epistatic interactions of several of the repair mutations with respect to 5-azaC-mediated DNA damage relative to UV-mediated DNA damage.
Hegde
V
V
Department of Molecular Biology and Biotechnology, University of Sheffield, U.K.
McFarlane
R J
RJ
Taylor
E M
EM
Price
C
C
D000251:Adenosine Triphosphatases; D001374:Azacitidine / Q000633:toxicity*; D001426:Bacterial Proteins / Q000235:genetics; D004249:DNA Damage; D004260:DNA Repair; D004268:DNA-Binding Proteins / Q000187:drug effects / Q000235:genetics; D004352:Drug Resistance, Microbial / Q000235:genetics; D004706:Endodeoxyribonucleases; D029968:Escherichia coli Proteins; D005656:Fungal Proteins / Q000187:drug effects / Q000235:genetics; D051719:MutS DNA Mismatch-Binding Protein; D009154:Mutation; D009711:Nucleotides / Q000235:genetics; D051135:Rad51 Recombinase; D011836:Radiation Tolerance / Q000235:genetics; D011995:Recombination, Genetic; D029701:Saccharomyces cerevisiae Proteins; D012568:Schizosaccharomyces / Q000187:drug effects / Q000235:genetics*; D029702:Schizosaccharomyces pombe Proteins; D012689:Sequence Homology, Nucleic Acid; D014157:Transcription Factors
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; D005656:Fungal Proteins; D009711:Nucleotides; C063607:RAD18 protein, S cerevisiae; C084232:RHP51 protein, S pombe; C097298:Rad12 protein, Coprinus cinereus; C062214:SWI4 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D029702:Schizosaccharomyces pombe Proteins; D014157:Transcription Factors; C073998:RAD2 protein, S cerevisiae; D051135:Rad51 Recombinase; D004706:Endodeoxyribonucleases; D000251:Adenosine Triphosphatases; D051719:MutS DNA Mismatch-Binding Protein; C497175:MutS protein, E coli; D001374:Azacitidine