Effect of aqueous and nonaqueous fixatives on the quantitative estimation of collagen-proteoglycan interaction in tissue sections.
The present study was designed to assess the influence of aqueous and nonaqueous fixatives on the quantitative estimation of collagen-proteoglycan interaction in tissue sections. Tissues containing different collagen types and distinct sulfated proteoglycan classes were isolated from pig costal cartilage, human skin, and the inner muscular layer of dog small intestine and fixed using aqueous or nonaqueous methods. The results showed that the best fixation method was exposure to paraformaldehyde gas. When using aqueous fixatives, proteoglycans were lost to different degrees among the various tissues analyzed, reflecting differences in chemical properties of proteoglycan classes and/or in their interactions with other matrix components such as collagen.
Toledo
O M
OM
Department of Morphology, Escola Paulista de Medicina, São Paulo, Brazil.
Taniwaki
N N
NN
Saldiva
P H
PH
Montes
G S
GS
D000818:Animals; D003094:Collagen / Q000378:metabolism*; D004285:Dogs; D005404:Fixatives / Q000737:chemistry*; D005557:Formaldehyde / Q000737:chemistry*; D006801:Humans; D008867:Microtomy; D011108:Polymers / Q000737:chemistry*; D011509:Proteoglycans / Q000378:metabolism*; D013552:Swine
D005404:Fixatives; D011108:Polymers; D011509:Proteoglycans; D005557:Formaldehyde; D003094:Collagen; C003043:paraform