AT1-receptors and cellular actions of angiotensin II in neuronal cultures of stroke prone-spontaneously hypertensive rat brain.
AT1-receptors, its mRNA and cellular actions of angiotensin II (Ang II) have been compared between neuronal cultures of Wistar Kyoto (WKY) and stroke-prone spontaneously hypertensive (SP-SH) rat brains. Bmax for AT1-receptor binding is 2-fold higher and is associated with a parallel increase in the levels of AT1-receptor mRNA in SP-SH rat brain neurons compared with WKY rat brain neurons. Ang II causes stimulation of both c-fos and norepinephrine transporter (NET) mRNAs in both strains of neurons and this stimulation is also 2-3-fold higher in SP-SH rat brain neurons compared with WKY rat brain neurons. In contrast, Ang II stimulation of PAI-1 mRNA in SP-SH neurons is only 50% that of in WKY rat brain neurons suggesting that SP-SH neurons express a decrease in AT1-receptor coupling with PAI-1 response. These observations demonstrate that SP-SH neurons express AT1-receptor-functions similar to those described for SHR neurons.
Raizada
M K
MK
Department of Physiology, University of Florida, Gainesville, USA.
Lu
D
D
Yang
H
H
Yu
K
K
D000804:Angiotensin II / Q000494:pharmacology*; D000818:Animals; D001921:Brain / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D002478:Cells, Cultured; D002561:Cerebrovascular Disorders; D006973:Hypertension; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D050484:Norepinephrine Plasma Membrane Transport Proteins; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics / Q000378:metabolism; D051381:Rats; D011921:Rats, Inbred WKY; D044140:Receptor, Angiotensin, Type 1; D011945:Receptors, Angiotensin / Q000378:metabolism*; D027981:Symporters
D002352:Carrier Proteins; D050484:Norepinephrine Plasma Membrane Transport Proteins; D016760:Proto-Oncogene Proteins c-fos; D044140:Receptor, Angiotensin, Type 1; D011945:Receptors, Angiotensin; C493226:Slc6a2 protein, rat; D027981:Symporters; D000804:Angiotensin II