title: The e subunit gene of murine F1F0-ATP synthase. Genomic sequence, chromosomal mapping, and diet regulation.
abstract: Genomic sequences encoding murine Lfm1, whose predicted protein sequence is 96% and 98% similar to bovine and rat F1F0-ATP synthase e subunits (respectively), have been amplified from BALB/cByJ DNA, cloned, and sequenced. The 1.1-kilobase gene has 3 introns and 4 exons, and its coding sequence differs by two nucleotides compared to the previously published BALB/cHnn Lfm1 cDNA sequence. A PstI restriction site polymorphism in intron 2 between C57BL/6J and Mus spretus was used to map this gene to Chromosome 5 near D5Mit9. Related sequences were mapped on Chromosomes 8, 11, and 2 unlinked loci on Chromosome 2 using Southern blot analyses with the 1. 1-kilobase gene as probe. Previous studies from this laboratory indicated that the Lfm1/e subunit was regulated by the level of dietary fat and carbohydrate. Northern hybridization analyses demonstrated that e subunit mRNA abundance showed statistically significant differences (p < 0.025) between hearts of BALB/c mice fed 3% and those fed 20% corn oil for 2 weeks and in liver (p < 0. 05) from the same animals. Significant differences were also observed in hepatic and heart mRNA expression at different times after eating in animals subjected to a fast/refeed regimen. The implications of the high degree of sequence similarity to the e subunit for rat and bovine F1F0-ATP synthase and its regulation by diet are discussed.
authors.affiliation: Department of Internal Medicine, University of Illinois College of Medicine, University of Illinois, Urbana, 61801, USA.
authors.forename: D A
authors.initials: DA
authors.lastname: Swartz
authors.forename: E I
authors.initials: EI
authors.lastname: Park
authors.forename: W J
authors.initials: WJ
authors.lastname: Visek
authors.forename: J
authors.initials: J
authors.lastname: Kaput
mesh_terms: D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D004032:Diet; D004435:Eating; D015971:Gene Expression Regulation, Enzymologic; D005796:Genes; D008099:Liver / Q000201:enzymology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D009206:Myocardium / Q000201:enzymology; D012150:Polymorphism, Restriction Fragment Length; D006180:Proton-Translocating ATPases / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D013997:Time Factors
chemical_list: D012333:RNA, Messenger; D006180:Proton-Translocating ATPases