[The variable number of tandem repeats in the renin gene of rats and possible significance in hypertension].
The variable number of tandem repeats (VNTR) in the first intron of renin gene for the spontaneously hypertensive rat (SHR), its controls Wistar-Kyoto (WKY), renal hypertensive rat, and Sprague-Dawley rat (SD) were compared by polymerase chain reaction (PCR) method. An analysis of VNTR from WKY, Wistar and SD showed that there are two different renin gene alleles and three genetypes 2.0kb/2.0kb, 2.0kb/1.8kb, 1.8kb/1.8kb. The genetype from renal hypertensive rats is same as those seen in the normal controls. However, compared with the WKY, Wistar and SD genes, a "deletion" of approximately 1.0kb was found in the first intron of the SHR renin gene. Our results strongly suggest that the cause and mechanism of elevated blood pressure is complex, and the molecular basis of the genetic-prone hypertension is existed.
Zhang
M
M
Institute of Basic Medical Sciences, CAMS, Beijing.
Fang
F
F
Chen
L
L
D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000235:genetics; D006973:Hypertension / Q000235:genetics*; D006977:Hypertension, Renal / Q000235:genetics; D018598:Minisatellite Repeats; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D012083:Renin / Q000235:genetics*
D004247:DNA; D012083:Renin