title: Application of reversed-phase liquid chromatography and prepacked C18 cartridges for the analysis of oxytetracycline and related compounds.
abstract: The reversed-phase (RP) chromatographic separation of oxytetracycline (OTC) 4-epioxytetracycline (4-epiOTC), alpha-apooxytetracycline (alpha-apoOTC), and beta-apooxytetracycline (beta-apoOTC) has been accomplished on an Inertsil C8 column at ambient temperature. Using the simplex method of solvent optimization, a 0.1 M ammonium acetate buffer (pH 3.0)-acetonitrile-tetrahydrofuran (72.5:12.5:15, v/v/v) mobile phase was found to give excellent separation of the compounds. OTC, 4-epiOTC, alpha-apoOTC and beta-apoOTC were resolved in 35 min with calculated detection limits of 40, 20, 50 and 140 ng/ml, respectively. Solid-phase extraction (using RP C18 cartridges) of OTC and OTC degradation compounds from distilled water and porcine muscle was tested at four concentration levels ranging from 200 to 2000 ng/ml (g); overall mean recovery of OTC from distilled water and porcine tissue was greater than 90% and 70%, respectively.
authors.affiliation: Department of Applied Microbiology and Food Science, University of Saskatchewan, Saskatoon, Canada.
authors.forename: R W
authors.initials: RW
authors.lastname: Fedeniuk
authors.forename: S
authors.initials: S
authors.lastname: Ramamurthi
authors.forename: A R
authors.initials: AR
authors.lastname: McCurdy
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods; D009132:Muscles / Q000737:chemistry; D010118:Oxytetracycline / Q000031:analogs & derivatives / Q000032:analysis*; D015203:Reproducibility of Results; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet; D013552:Swine
chemical_list: D000900:Anti-Bacterial Agents; D012997:Solvents; D010118:Oxytetracycline