Primary structure of a novel ABC transporter with a chromosomal localization on the band encoding the multidrug resistance-associated protein.
Complementary DNA clones encoding a novel protein, ABC-C, with the typical structural features of the ABC transporter family were identified in a human medullary thyroid carcinoma cell line. The transporter consists of 1704 amino acid residues with two homologous repeats, each harboring six putative transmembrane helices and an ATP-binding cassette motif. The mRNA is expressed highest in normal lung, but also in varying amounts in other tissues and in C-cell carcinoma. The ABC-C gene is mapped on chromosome 16p13.3, in close physical proximity to another ABC transporter, the multidrug resistance-associated protein. This related protein is assumed to confer resistance to chemotherapeutic drugs in small cell lung carcinoma. The genomic clustering of both transporters, typical also for other members of the ABC family, supports the notion that ABC-C may be involved in development of resistance to xenobiotics.
Klugbauer
N
N
Institut für Pharmakologie und Toxikologie, Technischen Universität München, Germany.
Hofmann
F
F
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000235:genetics*; D018528:ATP-Binding Cassette Transporters / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics*; D000255:Adenosine Triphosphate / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D018276:Carcinoma, Medullary; D002874:Chromosome Mapping; D002885:Chromosomes, Human, Pair 16; D002897:Chromosomes, Human, Pair 7; D002899:Chromosomes, Human, Pair 9; D003001:Cloning, Molecular; D018432:Drug Resistance, Multiple / Q000235:genetics; D015870:Gene Expression; D015723:Gene Library; D006801:Humans; D008168:Lung / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D017433:Protein Structure, Secondary; D012333:RNA, Messenger / Q000096:biosynthesis; D017386:Sequence Homology, Amino Acid; D013964:Thyroid Neoplasms; D014407:Tumor Cells, Cultured
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D018528:ATP-Binding Cassette Transporters; D012333:RNA, Messenger; D000255:Adenosine Triphosphate