Allergy to olive pollen: T-cell response from olive allergic patients is restricted by DR7-DQ2 antigens.
BACKGROUND
We have recently described the association between the IgE antibody response to Ole e I (the major antigen from olive tree pollen) and the DR7-DQ2 haplotype in a Spanish population.


OBJECTIVE
Due to the linkage disequilibrium between DR7 and DQ2, and thus the difficult distinction between the role of these two antigens in the T-cell activation response, we decided to solve this question by two approaches: 1. The study of another ethnic group, individuals of Arabic origin, with a presumably distinct disequilibrium linkage between DR and DQ antigens. Genomic DNA typing was performed in 46 subjects (allergic and non-allergic) by Restriction Fragment Length Polymorphism (RFLP) and results showed that patients with specific IgE antibodies alpha-Ole e I, were DR7 and/or DQ2. These data show a similar restriction pattern to those previously described for Spanish patients. The phenotypic frequency of DR7 antigen is significantly greater than in the non-allergic population, with a corrected P (Pc) value of 0.03. 2. The analysis of the genetic requirements of Ole e I response, using T-cell lines specific for this antigen. This was first carried out by blocking the proliferative response of these T-cell lines with specific anti-human HLA class II antibodies and then testing the genetic restriction of this response using a panel of histocompatible and histoincompatible Antigen Presenting Cells (APCs). Both experiments corroborate the hypothesis that DR7 and DQ2 are implicated in the recognition of Ole e I.
Cárdaba
B
B
Department of Immunology, Fundación Jiménez Díaz, Madrid, Spain.
De Pablo
R
R
Vilches
C
C
Martín
E
E
Geller-Bernstein
C
C
De Andrés
B
B
Zaharan
Y
Y
Del Pozo
V
V
Gallardo
S
S
De Arruda Chaves
E
E
Waisel
Y
Y
Palomino
P
P
Kreisler
M
M
Lahoz
C
C
D000293:Adolescent; D000328:Adult; D018912:Arabs; D002478:Cells, Cultured; D002648:Child; D002675:Child, Preschool; D006683:HLA-DQ Antigens / Q000235:genetics / Q000276:immunology*; D015809:HLA-DR7 Antigen / Q000235:genetics / Q000276:immunology*; D006801:Humans; D006967:Hypersensitivity / Q000208:ethnology / Q000276:immunology*; D007073:Immunoglobulin E / Q000276:immunology; D007223:Infant; D007231:Infant, Newborn; D008875:Middle Aged; D011058:Pollen / Q000276:immunology; D012150:Polymorphism, Restriction Fragment Length; D013601:T-Lymphocytes / Q000276:immunology*
D006683:HLA-DQ Antigens; C053186:HLA-DQ2 antigen; D015809:HLA-DR7 Antigen; D007073:Immunoglobulin E