Protective effect of an acidic glycoprotein obtained from culture of Chlorella vulgaris against myelosuppression by 5-fluorouracil.
An acidic glycoprotein prepared from a culture of Chlorella vulgaris (CVS) was examined for its protective effect on 5-fluorouracil(5FU)-induced myelosuppression and indigenous infection in mice. Subcutaneous administration of CVS greatly reduced the mortality of non-tumor-bearing mice given a high dose of 5FU, and could increase the LD50 value of 5FU for these mice. After 5FU treatment, indigenous infection developed probably as a result of the impairment of the host defense system. CVS reduced the incidence of indigenous infections and this effect was attributable to the acceleration of recovery from 5FU-induced myelosuppression. Early recovery of hematopoietic stem cells, or cells responding to interleukin-3 or granulocyte/macrophage-colony-stimulating factor, was especially observed in the bone marrow of CVS-treated mice on days 4-9 after the injection of 5FU. When tumor-bearing mice were given CVS during treatment with 5FU, CVS prolonged the survival of mice without affecting the antitumor activity of 5FU. In addition, CVS was itself shown to exert an antitumor effect. These results suggested that CVS may be beneficial for the alleviation of side-effects in cancer chemotherapy without affecting the antitumor activity of the chemotherapeutic agent.
Konishi
F
F
Research Laboratories, Chlorella Industries Co. Ltd., Fukuoka, Japan.
Mitsuyama
M
M
Okuda
M
M
Tanaka
K
K
Hasegawa
T
T
Nomoto
K
K
D000818:Animals; D000964:Antimetabolites, Antineoplastic / Q000633:toxicity*; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001424:Bacterial Infections / Q000517:prevention & control; D001853:Bone Marrow / Q000187:drug effects*; D002708:Chlorella / Q000737:chemistry*; D003115:Colony-Stimulating Factors / Q000097:blood; D005260:Female; D005472:Fluorouracil / Q000633:toxicity*; D006023:Glycoproteins / Q000494:pharmacology*; D006412:Hematopoietic Stem Cells / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D009374:Neoplasms, Experimental / Q000188:drug therapy
D000964:Antimetabolites, Antineoplastic; D000970:Antineoplastic Agents; D003115:Colony-Stimulating Factors; D006023:Glycoproteins; D005472:Fluorouracil