Direct chiral resolution of 15-deoxyspergualin using a cellobiohydrolase liquid chromatographic column.
An LC method for the direct determination of the enantiomers of 15-deoxyspergualin was developed, optimized and validated. A commercially available LC column containing a cellulase enzyme (cellobiohydrolase I) stationary phase was used. The effect of various parameters on the separation and validation data are discussed.
Mayo
D J
DJ
Bristol-Myers Squibb Pharmaceutical Research Institute, New Brunswick, NJ 08903-0191, USA.
Tomasella
F P
FP
Noroski
J E
JE
D002480:Cellulase / Q000032:analysis; D043366:Cellulose 1,4-beta-Cellobiosidase; D002853:Chromatography, Liquid / Q000379:methods*; D006146:Guanidines / Q000032:analysis* / Q000737:chemistry; D007166:Immunosuppressive Agents / Q000032:analysis* / Q000737:chemistry; D008968:Molecular Conformation
D006146:Guanidines; D007166:Immunosuppressive Agents; D002480:Cellulase; D043366:Cellulose 1,4-beta-Cellobiosidase; C037258:gusperimus