Preparation of serotype specific antiserum against porcine adenoviruses in mice.
Serotyping of porcine adenoviruses needs type-specific antiserum. The injections of purified virions to SPF Balb/C mice, followed by an intraperitoneal injection of Ehrlich ascitic tumour cells, gave enough ascitic fluid containing practically applicable type-specific antibody.
Kadoi
K
K
Department of Veterinary Medicine, College of Agriculture and Veterinary Medicine, Nihon University, Kanagawa, Japan.
D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology / Q000302:isolation & purification*; D001202:Ascitic Fluid / Q000276:immunology; D002286:Carcinoma, Ehrlich Tumor; D002460:Cell Line; D002478:Cells, Cultured; D007106:Immune Sera / Q000276:immunology / Q000302:isolation & purification*; D007114:Immunization; D008297:Male; D017921:Mastadenovirus / Q000145:classification / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D012703:Serotyping; D013047:Specific Pathogen-Free Organisms; D013552:Swine / Q000276:immunology* / Q000821:virology; D014407:Tumor Cells, Cultured
D000914:Antibodies, Viral; D007106:Immune Sera