title: Bacterial and fungal flora of dust deposits in a pig building.
abstract: OBJECTIVE
  The purpose of the study was to investigate the bacterial and fungal flora of dust deposits in a newly built pig grower finisher building. Viable bacterial counts and microbial species found in a barn which had never housed pigs were compared with those in a barn housing 144 pigs.
  
  
  METHODS
  The quantitative streak plate method was used to measure viable bacterial counts on nutrient agar or sheep blood agar. Viable bacterial counts of the dust deposits were expressed as the number of colony forming units (CFUs)/mg of dust. Gram positive cocci and Gram negative bacilli were identified by an automated system. Identifications with a confidence interval > 90% were accepted at the species level. Fungi were identified to the genus level with slide culture preparations on cereal agar.
  
  
  RESULTS
  The lowest viable bacterial count (4.8 x 10(4)/mg of dust) was found in the barn with no pigs. In the barn with pigs the highest viable bacterial count (2.1 x 10(6)/mg of dust) was in dust from the top of a partition close to pig activity. Six species of bacteria or fungi were found in dust from the room with no pigs, whereas 22 different microorganisms were detected in dust from the room with pigs. With the exception of Enterobacter agglomerans no other species of the family Enterobacteriaceae was found in dust deposits in this new pig building. Twelve species of Gram positive bacteria were found in the room housing pigs.
  
  
  CONCLUSIONS
  The pig is not only a source but also a disperser of airborne bacteria in pig buildings. Speciation of the microbial flora in dust from the pig building suggests that many of the microorganisms were either of human or environmental origin. Nevertheless as some of these microorganisms are known opportunistic pathogens or allergens and because of the documented increased incidence of chronic respiratory symptoms in pig workers, precautions to reduce inhalation of microbial or dust particles by pig workers seem prudent.
authors.affiliation: Department of Microbiology, University of Saskatchewan, Saskatoon, Canada.
authors.forename: W T
authors.initials: WT
authors.lastname: Martin
authors.forename: Y
authors.initials: Y
authors.lastname: Zhang
authors.forename: P
authors.initials: P
authors.lastname: Willson
authors.forename: T P
authors.initials: TP
authors.lastname: Archer
authors.forename: C
authors.initials: C
authors.lastname: Kinahan
authors.forename: E M
authors.initials: EM
authors.lastname: Barber
mesh_terms: D000818:Animals; D001419:Bacteria / Q000254:growth & development*; D015169:Colony Count, Microbial; D004391:Dust; D004783:Environmental Microbiology; D005658:Fungi / Q000254:growth & development*; D006090:Gram-Negative Bacteria / Q000254:growth & development; D006094:Gram-Positive Bacteria / Q000254:growth & development; D006095:Gram-Positive Cocci / Q000254:growth & development; D006799:Housing, Animal; D013552:Swine
chemical_list: D004391:Dust