title: Immunogenicity and protective efficacy of a tuberculosis DNA vaccine.
abstract: Tuberculosis is the most widespread and lethal infectious disease affecting humans. Immunization of mice with plasmid DNA constructs encoding one of the secreted components of Mycobacterium tuberculosis, antigen 85 (Ag85), induced substantial humoral and cell-mediated immune responses and conferred significant protection against challenge with live M. tuberculosis and M. bovis bacille Calmette-Guérin (BCG). These results indicate that immunization with DNA encoding a mycobacterial antigen provides an efficient and simple method for generating protective immunity and that this technique may be useful for defining the protective antigens of M. tuberculosis, leading to the development of a more effective vaccine.
authors.affiliation: Department of Virology, Pasteur Institute, Brussels, Belgium.
authors.forename: K
authors.initials: K
authors.lastname: Huygen
authors.forename: J
authors.initials: J
authors.lastname: Content
authors.forename: O
authors.initials: O
authors.lastname: Denis
authors.forename: D L
authors.initials: DL
authors.lastname: Montgomery
authors.forename: A M
authors.initials: AM
authors.lastname: Yawman
authors.forename: R R
authors.initials: RR
authors.lastname: Deck
authors.forename: C M
authors.initials: CM
authors.lastname: DeWitt
authors.forename: I M
authors.initials: IM
authors.lastname: Orme
authors.forename: S
authors.initials: S
authors.lastname: Baldwin
authors.forename: C
authors.initials: C
authors.lastname: D'Souza
authors.forename: A
authors.initials: A
authors.lastname: Drowart
authors.forename: E
authors.initials: E
authors.lastname: Lozes
authors.forename: P
authors.initials: P
authors.lastname: Vandenbussche
authors.forename: J P
authors.initials: JP
authors.lastname: Van Vooren
authors.forename: M A
authors.initials: MA
authors.lastname: Liu
authors.forename: J B
authors.initials: JB
authors.lastname: Ulmer
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000917:Antibody Formation; D000942:Antigens, Bacterial / Q000235:genetics* / Q000276:immunology; D001500:BCG Vaccine / Q000008:administration & dosage / Q000276:immunology*; D016207:Cytokines / Q000276:immunology; D004269:DNA, Bacterial / Q000008:administration & dosage / Q000276:immunology*; D004195:Disease Models, Animal; D007111:Immunity, Cellular; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology; D014376:Tuberculosis / Q000517:prevention & control*; D014407:Tumor Cells, Cultured
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001500:BCG Vaccine; D016207:Cytokines; D004269:DNA, Bacterial; C065036:antigen 85, Mycobacterium bovis