Anti-actin antibodies in sera from patients with autoimmune liver diseases and patients with carcinomas by ELISA.
Smooth muscle antibodies (SMA) were initially detected in sera of patients with chronic active hepatitis (CAH). Subsequently, their presence was demonstrated in a wide variety of other diseases. SMA are a mixture of antibodies directed towards different cytoskeletal antigens. Sera with high titers of anti-actin antibodies (AAA), a subgroup of SMA, are most frequently found among patients with autoimmune chronic active hepatitis (CAH) and, to a lesser extent, among patients with primary biliary cirrhosis (PBC) and other diseases. It is therefore established that AAA are a reliable marker to autoimmune CAH. The purpose of this study was to determine the titer of AAA in sera of patients with liver, autoimmune diseases and carcinomas, using the enzyme-linked immunoabsorbent assay (ELISA) method. The results were expressed as the optical density (OD) of the examined sera divided by the OD of a sera from a healthy control (presented as percentages +/- standard deviation). Sera of 33 patients with cirrhosis, nine patients with autoimmune CAH, fifteen patients with non-autoimmune CAH, eight patients with PBC, 30 patients with Sjogren's syndrome, 60 patients with SLE, 142 patients with carcinomas of different kinds, and 34 healthy donors were examined for the presence of AAA by ELISA. Statistically significant (P < 0.003) higher titers of AAA were detected in patients with autoimmune CAH (57 +/- 23%) compared with the control group and to other groups of diseases. AAA titers in non-autoimmune CAH were not significantly higher compared to the control group. High titers of AAA were detected in 67% of the patients with autoimmune CAH, as compared with other diseases in which only up to 13% of the patients exhibited AAA positivity.


CONCLUSIONS
existence of higher levels of AAA noted in 67% of the patients with autoimmune CAH with regard to other groups of diseases, emphasizes the value of AAA as sensitive and specific markers, capable of characterizing the patients with autoimmune chronic active hepatitis.
Leibovitch
L
L
Department of Medicine B, Sheba Medical Center, Tel-Aviv University, Tel-Hashomer, Israel.
George
J
J
Levi
Y
Y
Bakimer
R
R
Shoenfeld
Y
Y
D000199:Actins / Q000737:chemistry / Q000276:immunology*; D001323:Autoantibodies / Q000097:blood* / Q000737:chemistry; D001327:Autoimmune Diseases / Q000276:immunology*; D001667:Binding, Competitive / Q000276:immunology; D002277:Carcinoma / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006521:Hepatitis, Chronic / Q000276:immunology; D006801:Humans; D008104:Liver Cirrhosis, Alcoholic / Q000276:immunology; D008105:Liver Cirrhosis, Biliary / Q000276:immunology; D008107:Liver Diseases / Q000276:immunology*; D008180:Lupus Erythematosus, Systemic / Q000276:immunology; D012859:Sjogren's Syndrome / Q000276:immunology
D000199:Actins; D001323:Autoantibodies