title: An investigation of the interaction between alcohol and fibroblasts in wound healing.
abstract: This study investigated the effect of clinical concentrations of alcohol on fibroblast function (proliferation and ECM synthesis) in vitro. Basal and TGF-beta-induced collagen synthesis was assayed in confluent cultures in serum-free medium at 48 h with a commercial collagen assay system. At concentrations of alcohol > 5%, fibroblast proliferation was significantly inhibited. Although noninhibitory, subclinical concentrations of alcohol failed to inhibit basal collagen synthesis (P > 0.1), they significantly decreased TGF-beta-induced collagen synthesis (P < 0.03). These data support the notion that the local, as well as the systemic, effects of alcohol are important in mediating delayed healing in alcoholic patients.
authors.affiliation: Department of Oral Surgery, Medicine and Pathology, University of Wales College of Medicine, Cardiff, UK.
authors.forename: P
authors.initials: P
authors.lastname: Stephens
authors.forename: T
authors.initials: T
authors.lastname: al-Khateeb
authors.forename: K J
authors.initials: KJ
authors.lastname: Davies
authors.forename: J P
authors.initials: JP
authors.lastname: Shepherd
authors.forename: D W
authors.initials: DW
authors.lastname: Thomas
mesh_terms: D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis; D016895:Culture Media, Serum-Free; D000431:Ethanol / Q000633:toxicity*; D016326:Extracellular Matrix Proteins / Q000096:biosynthesis; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D006801:Humans; D016212:Transforming Growth Factor beta / Q000037:antagonists & inhibitors / Q000494:pharmacology; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D016895:Culture Media, Serum-Free; D016326:Extracellular Matrix Proteins; D016212:Transforming Growth Factor beta; D000431:Ethanol; D003094:Collagen