title: Physiological aspects of Trypanosoma cruzi gene regulation during heat-shock.
abstract: Investigations on the conditions of heat-shock response in Trypanosoma cruzi, the agent of Chagas disease, showed that at 37 degrees C, one of the heat-shock temperatures employed, the parasites from 48 h culture do not display a classical response to the heat treatment, since a general increase in RNA and protein synthesis was detected. The classical heat-shock response was detected only at 40 degrees C. The data also suggest that the heat shock proteins (HSP) mRNA population is sufficient to maintain protein synthesis at a high rate for at least 1 h and, to maintain the same rate of response for a longer period, transcription is necessary. The half life of HSP 70 mRNA is less than 3 h at 37 degrees C. The protein synthesized during the first hour of the heat shock at 37 degrees C is stable for at least 24 h. The parasite seems to be able to reuse the stock of HSP mRNAs stored during the first thermal shock to respond to a second heat treatment. These data are discussed bearing in mind other cell types.
authors.affiliation: Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Brazil.
authors.forename: E F
authors.initials: EF
authors.lastname: De Carvalho
authors.forename: F T
authors.initials: FT
authors.lastname: De Castro
authors.forename: E
authors.initials: E
authors.lastname: Rondinelli
authors.forename: J F
authors.initials: JF
authors.lastname: Carvalho
mesh_terms: D000818:Animals; D005786:Gene Expression Regulation; D006360:Heat-Shock Proteins / Q000096:biosynthesis*; D006358:Hot Temperature; D012333:RNA, Messenger / Q000096:biosynthesis; D014349:Trypanosoma cruzi / Q000235:genetics* / Q000502:physiology*
chemical_list: D006360:Heat-Shock Proteins; D012333:RNA, Messenger