Fluorescent vesicle angiography with sodium fluorescein and indocyanine green.
OBJECTIVE
The authors evaluated the feasibility of merging free-dye angiography and the fluorescent vesicle technique to achieve the best characteristics of both.


METHODS
Fluorescent vesicles encapsulated with either indocyanine green or carboxyfluorescein were mixed with free indocyanine green or free sodium fluorescein, respectively, and imaged with a scanning laser ophthalmoscope in both an in vitro model and primate and rabbit models.


RESULTS
In the in vitro model of the sodium fluorescein combination, optimal viewing of vesicleen capsulated dye and free dye was at a ratio of 150:1; for indocyanine green, the ratio was 50:1. In vivo, high-quality fluorescent vesicle angiograms were obtained that demonstrated leakage of free dye from choroidal laser spots.


CONCLUSIONS
Free dye and fluorescent vesicles can be combined to obtain an angiogram with all of the advantages of a traditional angiogram, while allowing the operator to assess the changes in retinal or choroidal circulation directly.
Peyman
G A
GA
LSU Eye Center, Louisiana State University Medical Center School of Medicine, New Orleans 70112-2234, USA.
Moshfeghi
D M
DM
Moshfeghi
A A
AA
Khoobehi
B
B
D000818:Animals; D002829:Choroid / Q000098:blood supply; D004396:Coloring Agents / Q000008:administration & dosage*; D004337:Drug Carriers; D005240:Feasibility Studies; D019793:Fluorescein; D005451:Fluorescein Angiography / Q000379:methods*; D005452:Fluoresceins / Q000008:administration & dosage*; D005456:Fluorescent Dyes / Q000008:administration & dosage; D007208:Indocyanine Green / Q000008:administration & dosage*; D008081:Liposomes; D008253:Macaca mulatta; D008297:Male; D011817:Rabbits; D012039:Regional Blood Flow; D012171:Retinal Vessels / Q000502:physiology
D004396:Coloring Agents; D004337:Drug Carriers; D005452:Fluoresceins; D005456:Fluorescent Dyes; D008081:Liposomes; C024098:6-carboxyfluorescein; D007208:Indocyanine Green; D019793:Fluorescein