A novel biological aspect of ovarian oxytocin: gene expression of oxytocin and oxytocin receptor in cumulus/luteal cells and the effect of oxytocin on embryogenesis in fertilized oocytes.
Recently, several authors have reported the existence of oxytocin (OT) in mammalian granulosa-luteal cells after ovulation. The purpose of this study was to examine the evidence for gene expression and localization of OT and OT receptor (OTR) in the cumulus cells surrounding the oocytes. Cumulus cells with mature oocytes were obtained from experimental and clinical in vitro fertilization-embryo transfer (IVF-ET) programs. OT and OTR gene expression was analyzed with reverse transcriptase-polymerase chain reaction (RT-PCR) and RT-PCR/single strand conformation polymorphism (SSCP). OT gene expression was detected in mouse and human cumulus cells. The results of RT-PCR/SSCP showed that the structure of OT mRNA in cumulus cells was similar to that in the hypothalamus. Furthermore, OTR gene expression was clearly demonstrated in human cumulus cells, and a weak positive signal was observed in human oocytes. Immunocytochemical staining of OTR was clearly detected in human cumulus cells. The rate of mouse blastocyst development was significantly higher in the group cultured with OT than that without OT. These results are the first observations of simultaneous OT and OTR gene expression in cumulus cells, suggesting that ovarian OT might have some physiological role in the early stage of embryo development.
Furuya
K
K
Department of Obstetrics and Gynecology, National Defense Medical College, Saitama, Japan.
Mizumoto
Y
Y
Makimura
N
N
Mitsui
C
C
Murakami
M
M
Tokuoka
S
S
Ishikawa
N
N
Nagata
I
I
Kimura
T
T
Ivell
R
R
D000818:Animals; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D005314:Embryonic and Fetal Development / Q000187:drug effects*; D005260:Female; D005307:Fertilization in Vitro; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D008184:Luteal Cells / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D009865:Oocytes / Q000378:metabolism; D010121:Oxytocin / Q000235:genetics* / Q000494:pharmacology*; D016133:Polymerase Chain Reaction; D018045:Receptors, Oxytocin / Q000235:genetics*
D017931:DNA Primers; D018045:Receptors, Oxytocin; D010121:Oxytocin