Comparison of the genomic short regions of a vaccine strain (SA-2) and a virulent strain (CSW-1) of infectious laryngotracheitis virus (Gallid herpesvirus 1).
Restriction enzyme linkage maps were produced for the genomic short region of the virulent infectious laryngotracheitis virus (CSW-1 strain). After comparison with the equivalent restriction enzyme linkage maps for the infectious laryngotracheitis virus SA-2 strain (a vaccine strain), it was determined that the maps for the short regions of the two strains were identical, apart from a single section in each of the inverted terminal repeats. Each inverted terminal repeat of the SA-2 strain was discovered to contain 467 base pairs more DNA than the CSW-1 strain's inverted terminal repeats. This extra DNA was more precisely mapped entirely within the EcoRI fragments D and d of SA-2, which were found to form part of the SmaI fragments U and P of SA-2 and Q and b of SA-2 and to contain one SmaI restriction enzyme site.
Trist
H M
HM
CSIRO, Division of Animal Health, Animal Health Research Laboratory, Parkville, Australia.
Tyack
S G
SG
Johnson
M A
MA
Prideaux
C T
CT
Sheppard
M
M
D000818:Animals; D001483:Base Sequence; D002645:Chickens / Q000821:virology*; D004279:DNA, Viral / Q000235:genetics*; D006566:Herpesviridae Infections / Q000662:veterinary* / Q000821:virology; D001352:Herpesvirus 1, Gallid / Q000145:classification / Q000235:genetics*; D008969:Molecular Sequence Data; D011201:Poultry Diseases / Q000821:virology*; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D013045:Species Specificity
D004279:DNA, Viral