Modulation of human bronchial epithelial cell IIICS fibronectin mRNA in vitro.
Fibronectin (Fn) is an extracellular matrix glycoprotein which is involved in wound repair, including repair of injured airway epithelium. Bronchial epithelial cells (BECs) are known to produce Fn which has enhanced chemotactic activity compared to serum Fn. Alternative splicing of the Fn gene is an important mechanism by which cells regulate the production of Fn. Human BECs produce Fn which contains the EIIIA region, but the expression of IIICS region variants has not previously been reported. Our purpose was to better define the molecular characteristics of human BEC Fn by determining the expression of alternative splice variants of the IIICS region of Fn of human BECs in vitro. Human bronchial epithelial cells obtained from bronchoscopy were cultured. To examine the presence of IIICS messenger ribonucleic acid (mRNA) variants, we synthesized oligonucleotide primers complementary to the published human fibronectin complementary deoxyribonucleic acid (cDNA) sequence of the IIICS domain for use in polymerase chain reactions (PCR) with total ribonucleic acid (RNA) extracted from cultured human BECs. To examine the modulation of IIICS mRNA expression, the 428 base pair (bp) DNA fragment generated in the PCR was oligo-labelled with 32[P]-deoxycytidine triphosphate (dCTP) for use as a probe for Northern blot analysis. Human BECs were cultured in the presence and absence of transforming growth factor-beta (TGF-beta) and agents which influence cyclic adenosine monophosphate (cAMP) including isoproterenol and dibutryl cAMP (db-cAMP). Total RNA from cultures was extracted, electrophoresis performed, and Northern blots obtained. Blots were hybridized with IIICS probe, total Fn cDNA, and tubulin cDNA. It was found that human BECs in culture expressed the five known human IIICS variants. TGF-beta enhanced the expression of IIICS mRNA in a concentration- and time-dependent fashion. Isoproterenol and db-cAMP both reduced the expression of IIICS mRNA and attenuated the TGF-beta induction. Changes in IIICS mRNA paralleled changes in total Fn mRNA, suggesting that these agents do not selectively modulate only the IIICS domain of Fn. We conclude that human airway epithelial cell Fn in vitro does contain mRNA for five IIICS variants, and that IIICS mRNA can be modulated by TGF-beta and agents which influence cAMP. It is unknown whether alterations in IIICS variants contribute to the functional differences previously observed between airway epithelial cell Fn and plasma-derived Fn.
Rosi
E
E
Clinica Medica III, Policlinico di Careggi, Firenze, Italy.
Beckmann
J D
JD
Pladsen
P
P
Rennard
S I
SI
Romberger
D J
DJ
D001483:Base Sequence; D001980:Bronchi / Q000166:cytology / Q000378:metabolism*; D002478:Cells, Cultured; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism; D005353:Fibronectins / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012326:RNA Splicing; D012333:RNA, Messenger / Q000235:genetics*; D016212:Transforming Growth Factor beta / Q000494:pharmacology
D005353:Fibronectins; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta