title: Striatal efferents preferentially innervate neurons in the ventral pallidum containing GABAA receptor alpha 1 subunit-like immunoreactivity.
abstract: The gamma-aminobutyric acid-A receptor consists of several subunits. In this immunohistochemical study we investigated the regional distribution of the alpha 1 and alpha 2 subunits with subunit-specific antibodies in the ventral pallidum, and compared the staining patterns to those of substance P (SP). alpha 1 subunit antigenic sites were found to be localized to pallidal neurons, varicosities, and varicose fibers. alpha 1 immunopositive fibers mainly appeared "tubulus-like" due to the intense staining of the membranes of the long pallidal dendrites. Double labelling of alpha 1 subunit and substance P revealed that alpha 1 subunit-like immunoreactive (IR) dendrites and somata of the pallidal neurons were often invested by SP-IR striatal efferents. Subcellularly, the dendritic and somatic membranes of pallidal neurons were strongly immunopositive for the alpha 1 subunit, whereas only a few axon terminals exhibited alpha 1-IR. alpha 2-IR was restricted to a low number of ventral pallidal neurons. The distributional patterns obtained for the alpha 1 and alpha 2 subunits suggest that striatal efferent neurons directly influence pallidal neurons displaying a distinct GABAA subunit composition, which may be of pharmacological importance since the alpha 1 beta x gamma 2-subunits containing receptors have mainly a benzodiazepine type I pharmacology.
authors.affiliation: Neurochemical Unit, Clinic of Psychiatry, Innsbruck, Austria.
authors.forename: J
authors.initials: J
authors.lastname: Marksteiner
authors.forename: W
authors.initials: W
authors.lastname: Kaufmann
authors.forename: K
authors.initials: K
authors.lastname: Pfaller
authors.forename: W
authors.initials: W
authors.lastname: Sieghart
authors.forename: A
authors.initials: A
authors.lastname: Saria
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D003342:Corpus Striatum / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D008854:Microscopy, Electron; D051381:Rats; D017207:Rats, Sprague-Dawley; D011963:Receptors, GABA-A / Q000378:metabolism*
chemical_list: D011963:Receptors, GABA-A