Differential expression of cGMP-inhibited cyclic nucleotide phosphodiesterases in human hepatoma cell lines.
PDE3 or cGMP-inhibited cyclic nucleotide phosphodiesterase (cGI PDE) activity was detected in homogenates of HepG2, Hep3B and HuH7, but not SK-Hep-1, human hepatoma cells. In HepG2 and Hep3B cells PDE3 activity was found predominantly in particulate fractions; in HuH7, in both particulate and supernatant fractions. cDNAs encoding two human PDE3s (an 'adipocyte' type, HcGIP1, and a 'cardiovascular' type, HcGIP2) have been cloned. HcGIP1 cDNA hybridized strongly with poly(A)+ RNA species from HepG2 and Hep3B. Both HcGIP1 and HcGIP2 mRNAs were expressed in Hep3B and HuH7 cells. The nucleotide sequence of an approximately 300-bp cDNA fragment, isolated after RT-PCR cloning from HepG2 RNA, was identical to a sequence within the conserved domain of HcGIP1 cDNA, consistent with the presence of HcGIP1 mRNA in HepG2 cells.
Murata
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Pulmonary/Critical Care Medicine Branch, NHLBI, NIH, Bethesda, MD 20892, USA.
Taira
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M
Manganiello
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VC
D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000096:biosynthesis / Q000378:metabolism*; D000230:Adenocarcinoma; D000273:Adipose Tissue / Q000201:enzymology; D001483:Base Sequence; D015152:Blotting, Northern; D006528:Carcinoma, Hepatocellular; D002460:Cell Line; D003001:Cloning, Molecular; D054684:Cyclic Nucleotide Phosphodiesterases, Type 3; D015342:DNA Probes; D018076:DNA, Complementary; D015870:Gene Expression; D006801:Humans; D007527:Isoenzymes / Q000096:biosynthesis / Q000378:metabolism*; D007700:Kinetics; D008099:Liver / Q000201:enzymology; D008113:Liver Neoplasms; D008969:Molecular Sequence Data; D009206:Myocardium / Q000201:enzymology; D010726:Phosphodiesterase Inhibitors / Q000494:pharmacology; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000096:biosynthesis; D013347:Subcellular Fractions / Q000201:enzymology; D014407:Tumor Cells, Cultured
D015342:DNA Probes; D018076:DNA, Complementary; D007527:Isoenzymes; D010726:Phosphodiesterase Inhibitors; D011994:Recombinant Proteins; D015105:3',5'-Cyclic-AMP Phosphodiesterases; D054684:Cyclic Nucleotide Phosphodiesterases, Type 3; C517287:PDE3A protein, human