Mapping of transregulatory domains of pseudorabies virus early protein 0 and identification of its dominant-negative mutant.
Pseudorabies virus (PRV) early protein 0 (EP0) is a transactivator containing the RING finger domain. Analysis of transactivating activity of truncated forms of the EP0 molecule consisting of 410 amino acids revealed that amino-terminal region containing the RING finger domain, amino acids 1 to 84, and the region between amino acids 114 to 242 containing acidic amino acid sequences were required for the transactivation. On the other hand, the mutant consisting of amino acids 1 to 113 exhibited a dominant-negative property.
Watanabe
S
S
Department of Disease Control, Hokkaido University Graduate School of Veterinary Medicine, Sapporo, Japan.
Ono
E
E
Shimizu
Y
Y
Kida
H
H
D000818:Animals; D002461:Cell Line, Transformed; D002522:Chlorocebus aethiops; D017781:Genes, Immediate-Early; D011558:Herpesvirus 1, Suid / Q000737:chemistry* / Q000235:genetics; D016296:Mutagenesis; D010449:Peptide Mapping; D015534:Trans-Activators / Q000737:chemistry* / Q000235:genetics; D014158:Transcription, Genetic; D015533:Transcriptional Activation; D014709:Vero Cells; D014764:Viral Proteins / Q000737:chemistry* / Q000235:genetics; D016335:Zinc Fingers
D015534:Trans-Activators; D014764:Viral Proteins; C070914:early protein O, Pseudorabies virus