[Effect of degree of aerosol OT reverse micelle hydration on the catalytic activity of peroxidase, its conjugate with cortisol and their immune complexes].
In Aerosol OT (AOT) reversed micelles in heptane, the oxidation kinetics of ortho-phenylenediamine (PDA) catalyzed by peroxidase (HRP), its conjugate with nine cortisol molecules and their immunocomplexes with monoclonal antibodies AT-2C specific to domain I of HRP has been studied with regard to the hydration degree of micelles W(0) = [H2O]/[A0T] and the concentration of solubilized biocatalysts. The profiles of dependencies of the initial PDA oxidation rate, v(0), on W(0) varied with an increase in the concentrations of HRP, HRP-cortisol and their immunocomplexes in micellar systems. The rise in HRP concentration from 0.5 up to 1.5 nM increases the v(0) values at a constant hydration degree, W(0) and is characterized by a limiting value of the initial rate at W(0) > 30. At HRP-cortisol concentrations of 1.2 and 1.5 nM, the dependences v(0)-W(0) have three maxima with gradually increasing values of the initial rate which may be accounted for by self-association of the hydrophobized conjugate, HRP-cortisol, in AOT micelles. The profiles of dependencies, v(0)-W(0) for HRP immunocomplexes (HRP-cortisol) with monoclonal antibodies, AT-2C, at various relationships of the components are characterized by three maxima with increasing values of v(0) which may by related to initial biocatalysts (HRP or HRP-cortisol) and their immunocomplexes with one or two molecules of AT-2C.
Karaseva
E I
EI
Cherednikova
T V
TV
Metelitsa
D I
DI
D000936:Antigen-Antibody Complex / Q000737:chemistry*; D002384:Catalysis; D004143:Dioctyl Sulfosuccinic Acid / Q000737:chemistry*; D006735:Horseradish Peroxidase / Q000737:chemistry* / Q000276:immunology; D006854:Hydrocortisone / Q000737:chemistry* / Q000276:immunology; D007124:Immunoenzyme Techniques; D008823:Micelles; D010655:Phenylenediamines / Q000737:chemistry; D014867:Water / Q000737:chemistry
D000936:Antigen-Antibody Complex; D008823:Micelles; D010655:Phenylenediamines; D014867:Water; D004143:Dioctyl Sulfosuccinic Acid; C034193:1,2-diaminobenzene; D006735:Horseradish Peroxidase; D006854:Hydrocortisone
Vliianie stepeni gidratatsii obrashchennykh mitsell aérozolia OT na kataliticheskuiu aktivnost' peroksidazy, ee kon''iugata s kortizolom i ikh immunnykh kompleksov.