[Photodynamic effect of HPD on DNA functions].
After treatment of the DNA by HPD and light the number of transformants of the M13mp8 DNA obviously decreased during the period of transforming the treated DNA into E coli. Meanwhile some of the mutation sequences appeared in the DNA which recovered from the DNA/HPD/Light transformant. It showed that the wrong replication happened because of the mutated template. Also the in vitro run-off transcription of IL-2R alpha gene was affected by HPD and light. It means that the DNA functions are influenced by the photodynamic effects.
Qi
Z
Z
Institute of Basic Medical Sciences, CAMS and PUMC, Beijing.
Zhao
X
X
Wang
Y
Y
Yan
J
J
Shen
Y
Y
D017104:Bacteriophage M13 / Q000235:genetics; D001483:Base Sequence; D004261:DNA Replication / Q000187:drug effects; D004269:DNA, Bacterial / Q000187:drug effects; D004279:DNA, Viral / Q000187:drug effects; D004926:Escherichia coli / Q000235:genetics; D017324:Hematoporphyrin Derivative / Q000494:pharmacology*; D008027:Light; D008969:Molecular Sequence Data; D009154:Mutation; D017319:Photosensitizing Agents / Q000494:pharmacology*; D015375:Receptors, Interleukin-2 / Q000235:genetics*; D014158:Transcription, Genetic / Q000187:drug effects*
D004269:DNA, Bacterial; D004279:DNA, Viral; D017319:Photosensitizing Agents; D015375:Receptors, Interleukin-2; D017324:Hematoporphyrin Derivative