title: Common occurrence of homologues of petunia glycine-rich protein-1 among plants.
abstract: The presence of specific glycine-rich proteins (GRP) related to petunia GRP1 (ptGRP1) was examined in three species of monocots (wheat, barley and maize) and five species of dicots (rape, turnip, soybean, crabapple and tomato). Protein blot analysis showed that anti-ptGRP1 antibody cross-reacted with a single different polypeptide in all species except maize. The molecular mass of these polypeptides ranged from 14 to 55 kDa. Tissue-print immunoblots of rape petioles and stems showed that the rape ptGRP1 homologue, like ptGRP1, is primarily located in the vascular tissue, and that its expression decreases with developmental age of the tissue. In barley, the ptGRP1 homologue is found in leaf vascular bundles, and may also be present in the surrounding bundle sheaths. Unlike the dicots examined, expression of the protein did not appear to decrease significantly with developmental age.
authors.affiliation: Department of Biochemistry/200, University of Nevada, Reno 89557, USA.
authors.forename: S H
authors.initials: SH
authors.lastname: Cheng
authors.forename: B
authors.initials: B
authors.lastname: Keller
authors.forename: C M
authors.initials: CM
authors.lastname: Condit
mesh_terms: D015153:Blotting, Western; D007106:Immune Sera; D010940:Plant Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D010944:Plants / Q000235:genetics / Q000378:metabolism; D013045:Species Specificity
chemical_list: D007106:Immune Sera; D010940:Plant Proteins; C070552:GRP protein, petunia