Purification of acid phosphatase I from germinating seeds of Vigna sinensis.
Acid phosphatase I (AP-I) is the major isoform of Vigna acid phosphatase. It is constitutively expressed in seed cotyledons during germination. AP-I was separated from other isoforms and purified to homogeneity by three simple purification steps; (NH4)2SO4 precipitation, and phosphocellulose and DEAE-cellulose column chromatography. The activity of AP-I was not affected by 1 mM Mg2+, Mn2+, Ca2+, Co2+ or Pb2+, but severely inhibited by 1 mM Cu2+, Fe3+, Hg2+, Mo6+ or Zn2+. AP-I has both phosphatase and pyrophosphatase activities, and is highly stable even at 50 degrees.
Biswas
T K
TK
Department of Pathology, University of Chicago, IL 60637, USA.
Promo
M
M
Biswas
B
B
D000135:Acid Phosphatase / Q000302:isolation & purification* / Q000378:metabolism*; D000645:Ammonium Sulfate; D002412:Cations / Q000494:pharmacology; D002848:Chromatography, DEAE-Cellulose; D002852:Chromatography, Ion Exchange; D007887:Fabaceae / Q000201:enzymology*; D007527:Isoenzymes / Q000302:isolation & purification* / Q000378:metabolism*; D007700:Kinetics; D010946:Plants, Medicinal; D012639:Seeds
D002412:Cations; D007527:Isoenzymes; D000135:Acid Phosphatase; D000645:Ammonium Sulfate