A survey of the goat genome transcribed in the lactating mammary gland.
To fulfill its primary function, which is to synthesize milk during the course of lactation, the mammary gland requires efficient transcriptional, translational, and secretory machineries involving multiple genes among which promising candidates underlying the genetic variation of milk production have to be found. With the aim of providing a first transcriptional profile of lactating mammary tissue, a non-normalized cDNA library has been constructed from the udder of a lactating goat. After having discarded cDNA clones encoding the major milk proteins the rapid characterization of genes expressed in this tissue, by automated partial cDNA sequencing, was used to analyze a total of 435 cDNA clones. Examination of the Expressed Sequence Tags (ESTs) for similarities with sequence databases identified 234 cDNAs corresponding to 140 unique genes or proteins. Eighty-three clones, not similar to any current database entries, representing 77 novel sequences unrelated to previously described genes, were thus identified. Tissue specificity and relative abundance of 18 of these 77 unidentified clones were examined by dot blot and RT-PCR experiments. Sequence data were subsequently used to assign six genes of unknown localization in the bovine genome, to synteny groups by use of bovine-hamster cell hybrids and PCR.
Le Provost
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F
Laboratoire de Génétique Biochimique et de Cytogénétique, Institut National de la Recherche Agronomique, 78352 Jouy-en-Josas Cedex, France.
Lépingle
A
A
Martin
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P
D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D006224:Cricetinae; D017931:DNA Primers; D018076:DNA, Complementary; D004798:Enzymes / Q000235:genetics; D005260:Female; D015723:Gene Library; D006041:Goats / Q000235:genetics*; D006801:Humans; D006822:Hybrid Cells; D007774:Lactation; D008321:Mammary Glands, Animal / Q000378:metabolism*; D008894:Milk Proteins / Q000096:biosynthesis* / Q000235:genetics*; D008969:Molecular Sequence Data; D009928:Organ Specificity; D016133:Polymerase Chain Reaction / Q000379:methods; D011506:Proteins / Q000235:genetics; D014158:Transcription, Genetic
D017931:DNA Primers; D018076:DNA, Complementary; D004798:Enzymes; D008894:Milk Proteins; D011506:Proteins