Dorsal root ganglion neuron development in chick and rat.
The aim of this study was to compare the morphological development of dorsal root ganglion neurons in embryonic and early postnatal chicks and rats. The three-dimensional architecture of neurons was observed in ganglia in situ and in dissociated neurons by scanning electron microscopy after removal of the capsule and connective tissue. The percentages of neurons at different developmental stages were determined. The general morphological changes in the chick resembled those in the rat but the timing was different. In both chick and rat, the majority of neurons were bipolar at early stages of development (embryonic day 6 in chick and day 14 in rats) and later underwent pseudo-unipolarization to become mostly unipolar neurons at hatching or birth. This maturation event started at an earlier stage in chick embryos than in rats, with 57% unipolar neurons in chick and only 7% in rat on embryonic day 14. However, just after hatching or birth, at day 22 of development, a larger proportion of immature unipolar neurons remained in chicks (13%) than in rats (3%). We conclude that these differences should be taken into consideration in designing experiments on dorsal root ganglion neurons grown in tissue culture.
Matsuda
S
S
Department of Anatomy, Ehime University School of Medicine, Japan, matsuda@m.ehime-u.ac.jp
Baluk
P
P
Shimizu
D
D
Fujiwara
T
T
D000818:Animals; D000831:Animals, Newborn / Q000254:growth & development*; D002452:Cell Count; D002642:Chick Embryo / Q000196:embryology*; D005260:Female; D005727:Ganglia, Spinal / Q000196:embryology* / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D009046:Motor Neurons / Q000648:ultrastructure*; D011247:Pregnancy; D051381:Rats / Q000196:embryology*; D013045:Species Specificity