High performance liquid chromatography enables fast determination of ratios between individual proteolytic enzymes in human gastric mucosa.
The human gastric mucosa contains five isozymogens of pepsinogen (pepsinogens PGA1-PGA5), two isozymogens of gastricsinogen (gastricsinogens PGC6-PGC7) and zymogens of cathepsins. Ratios between some individual pepsins or pepsinogens are very important from a diagnostic point of view. The ratio of pepsinogen 3 to pepsinogen 5 is significant marker of gastric cancer and gastric ulcer. High-performance ion-exchange chromatography is an easy and fast method for determination of ratios between individual proteolytic zymogens in human gastric mucosa and thus could serve for additional diagnosis of gastric diseases.
Hynek
R
R
Department of Biochemistry and Microbiology, 1st Faculty of Medicine, Charles University; Prague, Czech Republic.
Sajdok
J
J
Kucerová
Z
Z
Kás
J
J
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005753:Gastric Mucosa / Q000201:enzymology*; D006801:Humans; D010434:Pepsin A / Q000032:analysis*; D010435:Pepsinogens / Q000032:analysis*; D013276:Stomach Ulcer / Q000201:enzymology; D013552:Swine
D010435:Pepsinogens; D010434:Pepsin A