title: Use of macromolecular assemblies as expression systems for peptides and synthetic vaccines.
abstract: The past decade has witnessed the development of numerous systems for the presentation of antigens on the surface of self-assembling macromolecules. Although the sites for insertion were initially chosen empirically, the determination of the three-dimensional structures of a number of carrier macromolecules has enabled structure-based insertional mutagenesis to be used increasingly. Furthermore, it is now feasible to determine the structure of an inserted sequence as presented in a heterologous environment, making it possible to correlate the detailed structure of a peptide with its immunological properties.
authors.affiliation: Department of Virus Research, John Innes Centre, Norwich, UK. lomonossoff@bbsrc.ac.uk
authors.forename: G P
authors.initials: GP
authors.lastname: Lomonossoff
authors.forename: J E
authors.initials: JE
authors.lastname: Johnson
mesh_terms: D000818:Animals; D000939:Epitopes / Q000737:chemistry*; D006801:Humans; D046911:Macromolecular Substances; D010455:Peptides / Q000737:chemistry*; D014614:Vaccines, Synthetic / Q000096:biosynthesis* / Q000737:chemistry; D014764:Viral Proteins / Q000737:chemistry
chemical_list: D000939:Epitopes; D046911:Macromolecular Substances; D010455:Peptides; D014614:Vaccines, Synthetic; D014764:Viral Proteins