title: [Expression of the extracellular domain (1-341) of rat ovarian lutropin receptor in insect cells and preliminary characterization of the expressed product].
abstract: Extracellular domain residues 1-341 (designed R 341) of luteinizing hormone/human chorionic gonadotropin receptor (LH/hCG receptor) has high binding affinity for ligand. This paper describes expression of cDNA coding for R 341 in insect cells and preliminary identification of the expressed protein. SDS-PAGE silver staining and immunoblotting analysis show expressed product appears in two bands, major band has molecular weight 38.5 Kd, and weak band, 40.0 Kd. Ligand binding immunoblotting and 125I-hCG-binding blotting analysis indicate that expressed product R 341 has specific binding affinity for ligand. Ligand binding assay and Scatchard analysis indicate that recombinant receptor R 341 has high binding affinity for hCG and Kd is 5.68 x 10(-10) mol/L.
authors.affiliation: Shanghai Institute of Cell Biology, Academia Sinica.
authors.forename: Q X
authors.initials: QX
authors.lastname: Shen
authors.forename: C L
authors.initials: CL
authors.lastname: Li
authors.forename: H
authors.initials: H
authors.lastname: Shen
authors.forename: H H
authors.initials: HH
authors.lastname: Liu
authors.forename: Y G
authors.initials: YG
authors.lastname: Huang
authors.forename: W Y
authors.initials: WY
authors.lastname: Chen
authors.forename: O P
authors.initials: OP
authors.lastname: Bahl
mesh_terms: D000818:Animals; D016367:Baculoviridae / Q000235:genetics*; D001483:Base Sequence; D002478:Cells, Cultured; D018076:DNA, Complementary; D005260:Female; D015870:Gene Expression; D007313:Insecta / Q000166:cytology / Q000378:metabolism*; D008969:Molecular Sequence Data; D010053:Ovary / Q000378:metabolism; D010446:Peptide Fragments / Q000096:biosynthesis*; D011869:Radioligand Assay; D051381:Rats; D011966:Receptors, LHRH / Q000096:biosynthesis* / Q000235:genetics; D014162:Transfection
chemical_list: D018076:DNA, Complementary; D010446:Peptide Fragments; D011966:Receptors, LHRH