Production of monoclonal antibodies to study corrosion products of CO-CR biomaterials.
Sensitivity to nickel, cobalt, and chromium is common among the general population. The identification of these sensitivities is generally by the detection of cell-mediated immunity. We have reported previously the use of an indirect enzyme-linked immunosorbent assay method to quantitate metal-specific antibodies in patients with total joint replacements. To study the haptenic potential of these metal ions, rabbit albumin-glutathione-metal complexes with chromium, cobalt, or nickel were injected into mice. The splenocytes from one mouse in each group which developed a strong antibody against GSH-metal complexes were isolated and fused with myeloma cells to produce monoclonal antibodies. Chromium, cobalt, and nickel antibodies had similar affinity and bound with the specific GSH-metal complex. There was very little cross-reactivity between these antibodies. An inhibition assay using these monoclonal antibodies was demonstrated to be a simple technique, suitable for quantitation of free metal in solution.
Yang
J
J
Department of Biomedical Engineering, Case Western Reserve University, Cleveland, Ohio 44106, USA.
Merritt
K
K
D000818:Animals; D000911:Antibodies, Monoclonal; D001672:Biocompatible Materials; D002857:Chromium / Q000009:adverse effects / Q000032:analysis*; D003035:Cobalt / Q000009:adverse effects / Q000032:analysis*; D004342:Drug Hypersensitivity; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D004868:Equipment Failure; D005978:Glutathione; D006241:Haptens; D006801:Humans; D007595:Joint Prosthesis; D051379:Mice; D008807:Mice, Inbred BALB C; D009532:Nickel / Q000009:adverse effects / Q000032:analysis*; D011817:Rabbits; D012709:Serum Albumin
D000911:Antibodies, Monoclonal; D001672:Biocompatible Materials; D006241:Haptens; D012709:Serum Albumin; D002857:Chromium; D003035:Cobalt; D009532:Nickel; D005978:Glutathione