[Effect of plasma lipoproteins and apolipoprotein A-I on lipopolysaccharide-induced production of reactive oxygen metabolites by Kupffer cells of the rat].
The effects of plasma lipoproteins on LPS-induced production of reactive oxygen intermediates (ROI) in Kupffer cells isolated from livers of zymosan-treated rats, have been studied. ROI production was low in normal Kupffer cells but increased 18-fold by LPS in Kupffer cells from zymosan-treated rats. However, preincubation of LPS with rat serum, low density lipoproteins, high density lipoproteins (HDL3) and apolipoprotein A-I (apoA-I) decreased the LPS-induced production of ROI by 1.2-, 1.3-, 2.8-, and 1.5-fold, respectively. In contrast, very low density lipoproteins and HDL2 had no significant effect on the LPS-induced production of ROI. These results provide evidence for LPS-HDL3 complex formation and suggest that HDL3 and apoA-I play an important role in host defense against endotoxemia and infection.
Usynin
I F
IF
Tsyrendorzhiev
D D
DD
Khar'khovskiĭ
A V
AV
Poliakov
L M
LM
Panin
L E
LE
D000818:Animals; D016632:Apolipoprotein A-I / Q000097:blood*; D007728:Kupffer Cells / Q000187:drug effects* / Q000378:metabolism; D008074:Lipoproteins / Q000097:blood* / Q000145:classification; D008163:Luminescent Measurements; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species; D015054:Zymosan / Q000494:pharmacology*
D016632:Apolipoprotein A-I; D008074:Lipoproteins; D017382:Reactive Oxygen Species; D015054:Zymosan
Vliianie plazmennykh lipoproteinov i apolipoproteina A-I na lipopolisakharid-indutsirovannuiu produktsiiu reaktivnykh metabolitov kisloroda kupferovskimi kletkami krys.