title: Apoptosis in cell cultures induced by infectious bursal disease virus following in vitro infection.
abstract: Chicken embryo fibroblasts (CEFs) and Vero cells infected with infectious bursal disease virus (IBDV) exhibited the biochemical feature of apoptosis. Agarose gel electrophoresis of DNA extracted from IBDV-infected cells revealed the characteristic laddering pattern of DNA fragmentation, which was more intense in infected CEFs than in Vero cells. The appearance of apoptotic nucleosomal DNA fragments in IBDV-infected CEFs was independent of virus replication and occurred at an early stage following in vitro infection.
authors.affiliation: Virology Section, Central Animal Health Laboratory, Upper Hutt, New Zealand.
authors.forename: K M
authors.initials: KM
authors.lastname: Tham
authors.forename: C D
authors.initials: CD
authors.lastname: Moon
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics / Q000502:physiology*; D001483:Base Sequence; D018175:Birnaviridae Infections / Q000503:physiopathology / Q000662:veterinary*; D002478:Cells, Cultured; D002642:Chick Embryo; D002522:Chlorocebus aethiops; D004249:DNA Damage; D005347:Fibroblasts; D007243:Infectious bursal disease virus / Q000502:physiology; D008969:Molecular Sequence Data; D014709:Vero Cells; D014779:Virus Replication