title: Growth kinetics of Saccharomyces cerevisiae in batch and fed-batch cultivation using sugarcane molasses and glucose syrup from cassava starch.
abstract: Growth kinetics of Saccharomyces cerevisiae in glucose syrup from cassava starch and sugarcane molasses were studied using batch and fed-batch cultivation. The optimum temperature and pH required for growth were 30 degrees C and pH 5.5, respectively. In batch culture the productivity and overall cell yield were 0.31 g L-1 h-1 and 0.23 g cells g-1 sugar, respectively, on glucose syrup and 0.22 g L-1 h-1 and 0.18 g cells g-1 sugar, respectively, on molasses. In fed-batch cultivation, a productivity of 3.12 g L-1 h-1 and an overall cell yield of 0.52 g cells g-1 sugar were achieved in glucose syrup cultivation and a productivity of 2.33 g L-1 h-1 and an overall cell yield of 0.46 g cells g-1 sugar were achieved in molasses cultivation by controlling the reducing sugar concentration at its optimum level obtained from the fermentation model. By using an on-line ethanol sensor combined with a porous Teflon tubing method in automating the feeding of substrate in the fed-batch culture, a productivity of 2.15 g L-1 h-1 with a yield of 0.47 g cells g-1 sugar was achieved using glucose syrup as substrate when ethanol concentration was kept at a constant level by automatic control.
authors.affiliation: School of Environment, Resources and Development, Asian Institute of Technology, Bangkok, Thailand.
authors.forename: S S
authors.initials: SS
authors.lastname: Win
authors.forename: A
authors.initials: A
authors.lastname: Impoolsup
authors.forename: A
authors.initials: A
authors.lastname: Noomhorm
mesh_terms: D000431:Ethanol / Q000032:analysis; D005285:Fermentation; D005947:Glucose; D007700:Kinetics; D002365:Manihot; D008965:Molasses; D010945:Plants, Edible; D012441:Saccharomyces cerevisiae / Q000254:growth & development*; D013213:Starch; D013997:Time Factors
chemical_list: D000431:Ethanol; D013213:Starch; D005947:Glucose