title: Evaluation of commercially available ELISA test kits for the detection of clenbuterol and other beta 2-agonists.
abstract: This article presents the results of a comparative study of nine commercially available ELISA test kits for clenbuterol and other beta 2-agonists currently being used in veterinary drug residue control of live and slaughtered animals in the European Union (EU). By determining measuring ranges, B/B0-50% values, inter- and intra-assay variations and cross-reactions as well as limits of detection (LOD) and recoveries for clenbuterol in bovine urine it could be demonstrated that a number of test kits showed considerable quality defects, reducing their applicability to residue control. LODs for urine assayed without a previous clean-up (as recommended by all kit manufacturers) varied between 1.2 and 11.1 ng clenbuterol per ml urine and did thus not meet the requirement of 1 ng/ml of the official residue control plans of the EU Member States.
authors.affiliation: EU Reference Laboratory for Residues of Veterinary Drugs, Federal Institute for Health Protection of Consumers and Veterinary Medicine, Berlin, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Hahnau
authors.forename: B
authors.initials: B
authors.lastname: Jülicher
mesh_terms: D000318:Adrenergic beta-Agonists / Q000652:urine*; D000818:Animals; D002417:Cattle; D002976:Clenbuterol / Q000652:urine*; D004350:Drug Residues / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay / Q000706:statistics & numerical data; D005260:Female; D012680:Sensitivity and Specificity; D014730:Veterinary Medicine
chemical_list: D000318:Adrenergic beta-Agonists; D002976:Clenbuterol