title: Antigen association of total HEp-2 cell extract in the screening of autoimmune diseases by western blot.
abstract: Sera from patients with autoimmune diseases and healthy individuals were comparatively analyzed by Western blot, using total extract from HEp-2 cells as antigen. Bands between 14 and 20 kDa were present in 99% of patients with autoimmune diseases and symptomatic seronegative patients, in contrast to only 4% of healthy controls. No bands were detected in the remaining 96% of healthy controls. We propose these antigens as pathologically important for the diagnostic screening of patients with suspected autoimmune disease.
authors.affiliation: Laboratorio de Inmunología y Reumatología, Hospital de Especialidades Centro Médico La Raza, México.
authors.forename: N
authors.initials: N
authors.lastname: Capín
authors.forename: C
authors.initials: C
authors.lastname: Lavalle
authors.forename: L
authors.initials: L
authors.lastname: Ortiz-Ortiz
mesh_terms: D001323:Autoantibodies / Q000097:blood* / Q000276:immunology; D001327:Autoimmune Diseases / Q000175:diagnosis* / Q000276:immunology; D015153:Blotting, Western; D002457:Cell Extracts / Q000276:immunology; D003429:Cross Reactions; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000175:diagnosis / Q000276:immunology; D008970:Molecular Weight; D014407:Tumor Cells, Cultured
chemical_list: D001323:Autoantibodies; D002457:Cell Extracts