Natural human interferon-alpha enhances the expression of intracellular adhesion molecule-1, integrin alpha 2 and beta 1 by a mucosal epithelial cell line.
We have found that pretreatment of a human oral mucosal epithelial cell line KB with natural human interferon-alpha (IFN-alpha) augments the expression of intercellular adhesion molecule-1 (ICAM-1), and the complex of CD29 and CD49b, which is known as very late antigen (VLA)-2, in a dose-dependent manner, whereas the expression of other adhesion molecules such as CD44 leukocyte function associated antigen-3 (LFA-3), VLA-4 and endothelial leukocyte adhesion molecule-1 (ELAM-1) did not show any significant changes under the same conditions. The pretreatment of KB cells with IFN-alpha also induces the adhesion of these cells to the human leukemia T cell line MOLT-16 and to peripheral T lymphocytes in a dose-dependent manner. However, the adhesion of the above-mentioned T cells to KB cells was not inhibited by specific antibodies against ICAM-1, CD29, and CD49b. The results thus show that adhesion molecules other than ICAM-1, CD29, AND CD49b are responsible for the induced adhesion between T cells and IFN-alpha-pretreated KB cells.
Dao
T
T
Fujisaki Institute, Hayashibara Biochemical Laboratories, Inc., Okayama, Japan.
Takeuchi
M
M
Fukuda
S
S
Kurimoto
M
M
D015703:Antigens, CD / Q000096:biosynthesis*; D002448:Cell Adhesion; D002460:Cell Line; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism; D006801:Humans; D039421:Integrin alpha2; D019012:Integrin beta1 / Q000096:biosynthesis*; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis*; D016898:Interferon-alpha / Q000494:pharmacology*; D009061:Mouth Mucosa / Q000166:cytology / Q000378:metabolism*; D013601:T-Lymphocytes / Q000378:metabolism / Q000473:pathology
D015703:Antigens, CD; D039421:Integrin alpha2; D019012:Integrin beta1; D016898:Interferon-alpha; D018799:Intercellular Adhesion Molecule-1