title: Magnetic resonance imaging for the evaluation of rejection of a kidney allograft in the rat.
abstract: Orthotopic DA (RT1a) into Lewis (RT1l) rat kidney allografts and control Lewis-into-Lewis grafts were assessed by magnetic resonance imaging (MRI) and perfusion measurement after intravenous injection of a superparamagnetic contrast agent. MRI anatomical scores (range 1-6) and perfusion rates were compared with graft histology (rank of rejection score 1-6). Not only acute rejection, but also chronic events were monitored after acute rejection was prevented by daily cyclosporine (Sandimmune) treatment during the first 2 weeks after transplantation. In acute allograft rejection (n = 11), MRI scores reached the maximum value of 6 and perfusion rates were severely reduced within 5 days after transplantation; histology showed severe acute rejection (histologic score 5-6). In the chronic phase (100-130 days after transplantation), allografts (n = 5) manifested rejection (in histology cellular rejection and vessel changes), accompanied by MRI scores of around 2-3 and reduced perfusion rates. Both in the acute and chronic phases, the MRI anatomical score correlated significantly with the histological score (Spearman rank correlation coefficient rs 0.89, n = 30, P < 0.01), and perfusion rates correlated significantly with the MRI score or histological score (rs values between -0.60 and -0.87, n = 23, P < 0.01). It is concluded that MRI represents an interesting tool for assessing the anatomical and hemodynamical status of a kidney allograft in the acute and chronic phases after transplantation.
authors.affiliation: Sandoz Pharma Ltd., Basel, Switzerland.
authors.forename: N
authors.initials: N
authors.lastname: Beckmann
authors.forename: J
authors.initials: J
authors.lastname: Joergensen
authors.forename: K
authors.initials: K
authors.lastname: Bruttel
authors.forename: M
authors.initials: M
authors.lastname: Rudin
authors.forename: H J
authors.initials: HJ
authors.lastname: Schuurman
mesh_terms: D000208:Acute Disease; D000818:Animals; D002908:Chronic Disease; D003287:Contrast Media; D016572:Cyclosporine / Q000627:therapeutic use; D006084:Graft Rejection / Q000175:diagnosis* / Q000473:pathology; D007166:Immunosuppressive Agents / Q000627:therapeutic use; D007672:Kidney Cortex / Q000473:pathology; D016030:Kidney Transplantation / Q000276:immunology / Q000473:pathology*; D008279:Magnetic Resonance Imaging; D008297:Male; D010477:Perfusion; D051381:Rats; D011917:Rats, Inbred Lew; D011919:Rats, Inbred Strains; D013997:Time Factors; D014184:Transplantation, Homologous; D014185:Transplantation, Isogeneic
chemical_list: D003287:Contrast Media; D007166:Immunosuppressive Agents; D016572:Cyclosporine