Involvement of the mutated M protein in altered budding polarity of a pantropic mutant, F1-R, of Sendai virus.
Wild-type Sendai virus buds at the apical plasma membrane domain of polarized epithelial MDCK cells, whereas a pantropic mutant, F1-R, buds at both the apical and basolateral domains. In F1-R-infected cells, polarized protein transport and the microtubule network are impaired. It has been suggested that the mutated F and/or M proteins in F1-R are responsible for these changes (M. Tashiro, J. T. Seto, H.-D. Klenk, and R. Rott, J. Virol. 67:5902-5910, 1993). To clarify which gene or mutation(s) was responsible for the microtubule disruption which leads to altered budding of F1-R, MDCK cell lines containing the M gene of either the wild type or F1-R were established. When wild-type M protein was expressed at a level corresponding to that synthesized in virus-infected cells, cellular polarity and the integrity of the microtubules were affected to some extent. On the other hand, expression of the mutated F1-R M protein resulted in the formation of giant cells about 40 times larger than normal MDCK cells. Under these conditions, the effects on the microtubule network were enhanced. The microtubules were disrupted and polarized protein transport was impaired as indicated by the nonpolarized secretion of gp80, a host cell glycoprotein normally secreted from the apical domain, and bipolar budding of wild-type and F1-R Sendai viruses. The mutated F glycoprotein of F1-R was transported bipolarly in cells expressing the F1-R M protein, whereas it was transported predominantly to the apical domain when expressed alone or in cells coexpressing the wild-type M protein. These findings indicate that the M protein of F1-R is involved in the disruption of the microtubular network, leading to impairment of cellular polarity, bipolar transport of the F glycoprotein, and bipolar budding of the virus.
Tashiro
M
M
Department of Virology 1, National Institute of Health, Tokyo, Japan. mtashiro@nih.go.jp
McQueen
N L
NL
Seto
J T
JT
Klenk
H D
HD
Rott
R
R
D000818:Animals; D000906:Antibodies; D000911:Antibodies, Monoclonal; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002462:Cell Membrane / Q000821:virology; D002642:Chick Embryo; D004285:Dogs; D004848:Epithelium; D051379:Mice; D008870:Microtubules / Q000502:physiology / Q000648:ultrastructure; D009154:Mutation; D015739:Nocodazole / Q000494:pharmacology; D010222:Parainfluenza Virus 1, Human / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D011817:Rabbits; D014763:Viral Matrix Proteins / Q000235:genetics / Q000378:metabolism*; D015032:Zinc / Q000494:pharmacology
D000906:Antibodies; D000911:Antibodies, Monoclonal; C097020:M protein, Sendai virus; D014763:Viral Matrix Proteins; D015032:Zinc; D015739:Nocodazole