[Characterization of chick embryo fibroblast culture--overview of its effectiveness as a hybridoma cell].
The use of culture supernatant (added in a final concentration of 25% v/v) from chicken embryonal fibroblasts (CEF) for stimulation of established hybridoma cell lines has been shown. The proliferative effect on hybridoma cells from different origins was heatproof and was possible with a reduced serum part (FCS) in the culture medium. After a chromatografic separation the supernatant sections were tested and the positive effect was much lower in comparison to the total conditioned media.
Hlinak
A
A
Institut für Virologie, Freien Universität Berlin.
Jahn
S
S
Mehl
M
M
Grunow
R
R
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D002455:Cell Division; D002460:Cell Line; D002478:Cells, Cultured; D002642:Chick Embryo; D003470:Culture Media; D017077:Culture Media, Conditioned; D046508:Culture Techniques / Q000379:methods; D005347:Fibroblasts / Q000166:cytology / Q000276:immunology; D006825:Hybridomas / Q000166:cytology; D007700:Kinetics
D000911:Antibodies, Monoclonal; D003470:Culture Media; D017077:Culture Media, Conditioned
Charakterisierung von Hühnerembryofibroblastenkultur-Uberstand und seine Wirkung auf Hybridomzellen.