Molecular detection and differentiation of enteroviruses in endomyocardial biopsies and pericardial effusions from dilated cardiomyopathy and myocarditis.
Enteroviruses (EVs), especially group B coxsackieviruses, have been implicated in the pathogenesis of myocarditis and dilated cardiomyopathy (DCM). To determine whether a specific type of EV is present in DCM hearts, we examined the genotypes of EVs detected in endomyocardial biopsies and pericardial effusions by polymerase chain reaction-single-strand conformation polymorphism (PCR-SSCP) analysis. Positive PCR results were obtained from biopsies in 6 (19 percent) of 31 patients with DCM, 5 (18 percent) of 28 with myocarditis, 5 (22 percent) of 23 with other cardiac diseases, and from pericardial effusions in 4 (57 percent) of 7 patients with pericarditis. SSCP profiles of most of the clinical samples were different and were not identical to any of the standard group B coxsackie viruses. Our findings suggest that EV genomes are involved in the myocardium of patients with various cardiac conditions and that a particular type of EV is not present in DCM hearts.
Fujioka
S
S
Third Division, Department of Internal Medicine, Osaka Medical College, Japan.
Koide
H
H
Kitaura
Y
Y
Deguchi
H
H
Kawamura
K
K
Hirai
K
K
D000293:Adolescent; D000328:Adult; D000368:Aged; D001483:Base Sequence; D001706:Biopsy; D015139:Blotting, Southern; D002311:Cardiomyopathy, Dilated / Q000150:complications / Q000821:virology*; D002648:Child; D004699:Endocardium / Q000821:virology; D004770:Enterovirus / Q000235:genetics / Q000302:isolation & purification*; D005838:Genotype; D006321:Heart / Q000821:virology*; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009205:Myocarditis / Q000150:complications / Q000821:virology*; D010490:Pericardial Effusion / Q000209:etiology / Q000821:virology*; D016133:Polymerase Chain Reaction / Q000379:methods