title: Production of influenza virus in cell cultures for vaccine preparation.
abstract: Influenza virus strains of different types for use as an inactivated vaccine have been successfully grown in different cell lines. Increasing titres were obtained with BHK-21/BRS, VERO and MDCK cells. Cultures in stationary flasks, in spinner cultures or in large bioreactor systems were tested and the optimal conditions were studied. MDCK cells grown in serum-free medium before and during the virus production phase were found to yield high titres in the presence of trypsin. Satisfactory results were obtained with egg-adapted strains of human and equine origin as well as with strains just isolated from human patients without any further passages in eggs or cell culture.
authors.affiliation: Laboratoire de Technologie Cellulaire, Institut Pasteur, Paris, France.
authors.forename: O W
authors.initials: OW
authors.lastname: Merten
authors.forename: C
authors.initials: C
authors.lastname: Hannoun
authors.forename: J C
authors.initials: JC
authors.lastname: Manuguerra
authors.forename: F
authors.initials: F
authors.lastname: Ventre
authors.forename: S
authors.initials: S
authors.lastname: Petres
mesh_terms: D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D006224:Cricetinae; D046508:Culture Techniques / Q000379:methods; D004285:Dogs; D006736:Horses; D006801:Humans; D009980:Influenza A virus / Q000254:growth & development / Q000276:immunology* / Q000302:isolation & purification; D007252:Influenza Vaccines; D014709:Vero Cells; D014773:Virology / Q000379:methods; D014779:Virus Replication
chemical_list: D007252:Influenza Vaccines