title: [Simplified method of cultured brain microcapillary endothelial cells].
abstract: Many kinds of methods cultured brain microcapillary endothelial cells have been published, but these methods were not established. We designed the simplified methods with improvement of enzymatic treatment and Percoll gradient centrifugation.
authors.affiliation: Department of Neurosurgery, Kansai Medical University, Osaka, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Suyama
authors.forename: T
authors.initials: T
authors.lastname: Tsuchida
authors.forename: K
authors.initials: K
authors.lastname: Matsumura
authors.forename: N
authors.initials: N
authors.lastname: Sakai
authors.forename: K
authors.initials: K
authors.lastname: Kawamoto
authors.forename: T
authors.initials: T
authors.lastname: Kishimoto
authors.forename: M
authors.initials: M
authors.lastname: Tada
mesh_terms: D000818:Animals; D001921:Brain / Q000098:blood supply*; D002196:Capillaries / Q000166:cytology; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D003584:Cytological Techniques; D004730:Endothelium, Vascular / Q000166:cytology*; D013552:Swine