[Gene therapy of human pancreatic carcinoma by recombinant retroviral vector expressing herpes simplex virus thymidine kinase gene].
A recombinant retroviral vector pNTK expressing the "suicide" gene herpes simplex virus thymidine kinase (HSV-TK) gene was constructed. The recombinant vector was packaged by PA317 cells and viral supernatant was used to infect the human pancreatic carcinoma cell line PC-2. After selection in G418, resistant colonies were identified and isolated. Cytotoxic effects of the non-toxic prodrug Acyclovir (ACV) or Ganciclovir (GCV) to the NTK transformant PC-2 cells was more than 90%, while that of the control PC-2 cells was less that 10%. Furthermore, the "bystander effect" of HSV-TK on PC-2 cells was also observed.
Li
H
H
Department of Pathology, PUMC hospital, CAMS, Beijing.
Liu
T
T
Wang
Z
Z
D000212:Acyclovir / Q000494:pharmacology; D000998:Antiviral Agents / Q000494:pharmacology; D004274:DNA, Recombinant / Q000235:genetics; D015774:Ganciclovir / Q000494:pharmacology; D006801:Humans; D010190:Pancreatic Neoplasms / Q000235:genetics* / Q000473:pathology; D010957:Plasmids; D012190:Retroviridae / Q000235:genetics*; D018139:Simplexvirus / Q000737:chemistry; D013937:Thymidine Kinase / Q000235:genetics* / Q000302:isolation & purification; D014162:Transfection; D014407:Tumor Cells, Cultured / Q000187:drug effects
D000998:Antiviral Agents; D004274:DNA, Recombinant; D013937:Thymidine Kinase; D015774:Ganciclovir; D000212:Acyclovir