title: Structural and immunochemical studies on the lipopolysaccharide of the 'T-antigen'-containing mutant Proteus mirabilis R14/1959.
abstract: In DOC-PAGE, lipopolysaccharide (LPS) of Proteus mirabilis R14/1959 (Rb-type) mutant showed a ladder-like migration pattern indicating the presence of a high molecular weight polysaccharide chain. The isolated polysaccharide, called T-antigen because of similarity with the T1 chain of Salmonella friedenau LPS, contained D-glucose, D-galacturonic acid (D-GalA), and D-GlcNAc in molar ratios 2:1:1 and was structurally different from the O-antigen of the parental S-strain P. mirabilis S1959 but identical to the O-antigen of another S-strain Proteus penneri 42. The importance of a D-GalA(L-Lys)-containing epitope, most likely present in the core region of LPS, and of GalA present in the T-antigen chain in manifesting the serological specificity of P. mirabilis R14/1959 were revealed using rabbit polyclonal homologous and heterologous R- and O-specific antisera and the appropriate antigens, including synthetic antigens which represent partial structures of various Proteus LPS.
authors.affiliation: Institute of Microbiology and Immunology, University of Lodz, Poland.
authors.forename: B
authors.initials: B
authors.lastname: Bartodziejska
authors.forename: J
authors.initials: J
authors.lastname: Radziejewska-Lebrecht
authors.forename: M
authors.initials: M
authors.lastname: Lipinska
authors.forename: Y A
authors.initials: YA
authors.lastname: Knirel
authors.forename: L O
authors.initials: LO
authors.lastname: Kononov
authors.forename: A Y
authors.initials: AY
authors.lastname: Chernyak
authors.forename: H
authors.initials: H
authors.lastname: Mayer
authors.forename: A
authors.initials: A
authors.lastname: Rozalski
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000737:chemistry* / Q000276:immunology*; D015153:Blotting, Western; D002240:Carbohydrate Sequence; D003429:Cross Reactions; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000276:immunology*; D008969:Molecular Sequence Data; D009154:Mutation / Q000276:immunology*; D019081:O Antigens / Q000737:chemistry / Q000276:immunology; D011513:Proteus mirabilis / Q000235:genetics* / Q000276:immunology*; D011817:Rabbits
chemical_list: D000942:Antigens, Bacterial; D008070:Lipopolysaccharides; D019081:O Antigens