Evaluation of different absorbance ratios from infrared spectroscopy for analyzing the degree of deacetylation in chitin.
Various acetylated chitosan derivatives and mixtures of chitin and chitosan, covering the range of the degree of deacetylation (DDA) from 0-100% were analyzed by 1H-NMR spectroscopy and infrared spectroscopy. The use of the 1070 cm-1 or 1030 cm-1 absorption band as an internal standard in the determination of DDA from the absorbance of the amide I bands at 1655 cm-1 and 1630 cm-1 or the amide II band at 1560 cm-1 was studied. There is a good correlation between the results from IR spectroscopy and those from 1H-NMR spectroscopy.
Shigemasa
Y
Y
Department of Materials Science, Faculty of Engineering, Tottori University, Japan.
Matsuura
H
H
Sashiwa
H
H
Saimoto
H
H
D000107:Acetylation; D000818:Animals; D003386:Brachyura / Q000737:chemistry; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002686:Chitin / Q000031:analogs & derivatives* / Q000737:chemistry*; D048271:Chitosan; D005069:Evaluation Studies as Topic; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D013055:Spectrophotometry, Infrared
D002686:Chitin; D048271:Chitosan