Immunocytochemical localization of actin in the nucleolus of rat oocytes.
In order to determine the localization of actin, growing and fully grown rat oocytes were immunocytochemically examined using a post-embedding ultrastructural protein-A gold technique. In quiescent oocytes, the nucleoplasm showed slightly lower levels of actin signal when compared to the surrounding cytoplasm. The highest levels of labeling were found on nucleoli showing a reticular type morphology. In oocytes at the diakinesis stage in which nucleolar compaction had occurred, the levels of labeling increased by 5-6 times those found in quiescent oocytes. Except for conspicuous accumulation of actin under the plasma membrane, compact nucleoli had significantly higher levels of labeling when compared with those found on the general cytoplasm, while the nucleoplasm with homogeneously dispersed chromatin showed significantly lower levels of associated actin signal than the general cytoplasm. In oocytes at metaphase I, the cytoplasmic region had comparable or lower levels of labeling than the cytoplasm of oocytes at diakinesis. The meiotic spindle embedded in material with medium electron density showed a similar level of labeling as the surrounding cytoplasm. On the other hand, significantly higher levels of associated actin were observed on the chromosomes of metaphase I. The actin signals were dispersed over the chromosomes and not concentrated on a specific region. These results suggest that nuclear actin may be involved in the process of chromosome construction and also the formation of the compacted structure of the nucleolus.
Funaki
K
K
Department of Anatomy, Tottori University, Yonago, Japan.
Katsumoto
T
T
Iino
A
A
D000199:Actins / Q000032:analysis*; D000818:Animals; D002466:Cell Nucleolus / Q000648:ultrastructure*; D002875:Chromosomes / Q000648:ultrastructure; D005260:Female; D005456:Fluorescent Dyes; D008540:Meiosis; D016253:Microscopy, Immunoelectron; D009865:Oocytes / Q000166:cytology / Q000502:physiology / Q000648:ultrastructure*; D051381:Rats; D017208:Rats, Wistar; D008941:Spindle Apparatus / Q000648:ultrastructure
D000199:Actins; D005456:Fluorescent Dyes