Preliminary crystallographic studies of an anti-HIV-1 protease antibody that inhibits enzyme activity.
F11.2.32, a monoclonal antibody directed against the HIV-1 protease, displays strong inhibitory effects toward the catalytic activity of the enzyme. The antibody cross-reacts with peptides 36-46 and 36-57 from the protease. Crystals of the Fab have been obtained both in the free state and as complexes formed with the protease peptide fragments, 36-46 and 36-57. Diffraction data have been collected for the free and complexed forms of Fab F11.2.32 and preliminary models for the crystal structures were obtained by molecular replacement.
Lescar
J
J
Unité d'Immunologie Structurale, Institut Pasteur, Paris, France.
Stouracova
R
R
Riottot
M M
MM
Chitarra
V
V
Brynda
J
J
Fabry
M
M
Horejsi
M
M
Sedlacek
J
J
Bentley
G A
GA
D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry* / Q000276:immunology / Q000494:pharmacology; D003429:Cross Reactions; D018360:Crystallography, X-Ray; D015483:HIV Antibodies / Q000737:chemistry* / Q000276:immunology / Q000494:pharmacology; D015488:HIV Antigens / Q000276:immunology*; D016333:HIV Protease / Q000276:immunology*; D017320:HIV Protease Inhibitors / Q000737:chemistry* / Q000276:immunology; D015497:HIV-1 / Q000201:enzymology* / Q000276:immunology; D007140:Immunoglobulin Fab Fragments / Q000737:chemistry / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D010446:Peptide Fragments / Q000276:immunology; D011993:Recombinant Fusion Proteins / Q000276:immunology
D000911:Antibodies, Monoclonal; D015483:HIV Antibodies; D015488:HIV Antigens; D017320:HIV Protease Inhibitors; D007140:Immunoglobulin Fab Fragments; D010446:Peptide Fragments; D011993:Recombinant Fusion Proteins; D016333:HIV Protease