[Whole cell recording of sodium, potassium and calcium currents of cultured neonatal rat sympathetic neurons].
Na, K and Ca currents and other electrophysiological characteristics of cultured neonatal rat superior cervical sympathetic neurons were studied using whole cell clamp technique. The mean passive and active membrane properties measured are as follows: resting membrane potential, -51 +/- 6 mV; input resistance, 1432 +/- 389 M omega; time constant, 130 +/- 32 ms; amplitude of action potential, 96 +/- 10 mV; overshoot, 42 +/- 6 mV. Na, K and Ca currents were isolated upon pharmacological manipulations. The predominant type of K current was a noninactivating delayed rectifier. Voltage-clamp studies also showed the presence of a high voltage-activated sustained inward Ca current, while low voltage could not elicit any transient Ca current.
He
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Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing.
Liu
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QS
Xie
Z P
ZP
Liu
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CG
D000818:Animals; D000831:Animals, Newborn; D015220:Calcium Channels / Q000502:physiology; D002478:Cells, Cultured; D008564:Membrane Potentials; D009474:Neurons / Q000166:cytology; D018408:Patch-Clamp Techniques; D015221:Potassium Channels / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D015222:Sodium Channels / Q000502:physiology; D017783:Superior Cervical Ganglion / Q000166:cytology*
D015220:Calcium Channels; D015221:Potassium Channels; D015222:Sodium Channels