One-step purification of recombinant proteins with the 6xHis tag and Ni-NTA resin.
Crowe
J
J
QIAGEN GmbH, Hilden, Germany.
Masone
B S
BS
Ribbe
J
J
D001426:Bacterial Proteins / Q000302:isolation & purification; D002846:Chromatography, Affinity / Q000379:methods*; D004926:Escherichia coli / Q000235:genetics; D005820:Genetic Testing / Q000379:methods; D005822:Genetic Vectors; D006639:Histidine; D009532:Nickel; D009571:Nitrilotriacetic Acid / Q000031:analogs & derivatives*; D009942:Organometallic Compounds; D010957:Plasmids / Q000235:genetics*; D011994:Recombinant Proteins / Q000302:isolation & purification*; D012116:Resins, Plant; D012995:Solubility
D001426:Bacterial Proteins; D009942:Organometallic Compounds; D011994:Recombinant Proteins; D012116:Resins, Plant; C088321:nickel nitrilotriacetic acid; D006639:Histidine; D009532:Nickel; D009571:Nitrilotriacetic Acid