title: Essential fatty acid uptake and metabolism in the developing rodent brain.
abstract: Studies were carried out to determine whether the brain takes up and metabolizes essential fatty acids during early postnatal development in rodents. Rats and mice were dosed with deuterium-labeled linoleic and linolenic acids either by intraperitoneal injection or by gavage. Animals were killed at different times thereafter, and organs were removed. Brains, livers, and blood were analyzed by gas chromatography--negative-ion-mass spectrometry for labeled fatty acids. To determine whether fatty acids were present in the brain apart from cerebral blood, a subset of animals was exsanguinated by perfusion with buffered saline, and the brain was then fractionated into subcellular components. Results demonstrated that the brain took up both labeled essential fatty acids within 8 h from the time of dosing. There was on average a greater uptake of linolenic acid into the cerebellum than into the cerebral cortex during the first 8 d of life in rats. The amount of linoleic acid taken into either region was similar, however. Docosahexaenoic acid intermediates, 20:5n-3 and 22:5n-3, were also found labeled in the brain. Time-course labeling experiments indicated that these intermediates may be converted to 22:6n-3 within the brain. A rise of labeled 22:6n-3 in the brain at 24 h appeared to be due to uptake of this fatty acid from the blood. The amount of labeled 22:6n-3 in the brain continued to increase beyond 24 h, and this did not appear to be correlated with its blood concentration. These results suggest that, during development in the rodent, different regions within the brain may vary in their capacity to synthesize 22:6n-3, and this may be correlated with regional growth rates.
authors.affiliation: Laboratory of Membrane Biochemistry and Biophysics, National Institute on Alcoholism and Alcohol Abuse, Rockville, MD 20852, USA.
authors.forename: R J
authors.initials: RJ
authors.lastname: Pawlosky
authors.forename: G
authors.initials: G
authors.lastname: Ward
authors.forename: N
authors.initials: N
authors.lastname: Salem
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000833:Animals, Suckling; D001921:Brain / Q000254:growth & development / Q000378:metabolism*; D003903:Deuterium; D005228:Fatty Acids, Essential / Q000097:blood / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000097:blood / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D051379:Mice; D051381:Rats; D017962:alpha-Linolenic Acid / Q000097:blood / Q000378:metabolism*
chemical_list: D005228:Fatty Acids, Essential; D008041:Linoleic Acids; D017962:alpha-Linolenic Acid; D019787:Linoleic Acid; D003903:Deuterium