Filtration, centrifugation and mouse exposure for the detection of schistosome cercariae in water.
Three methods for detection of schistosome cercariae in water were studied under laboratory and simulated field conditions in Egypt. Water samples tested by filtration and centrifugation quantitatively reflected the density of cercariae in moderately turbid canal water (30 Nephelometry Turbidity Unites, NTU). When tested in waters of increasing turbidity, the centrifuge gave more consistent results than the filtration system. The centrifugation technique was more efficient in processing the water samples and took almost half the time of filtration. Mouse exposure was useful in detecting cercariae, but was not quantitative. However, there was a significant improvement in worm recovery when mice were more close together in the floatation device. Mouse exposure was also expensive and cumbersome to use. It appears from the present results that the technique of choice for further cercariometric work under Egyptian conditions may be centrifugation.
Yousif
F
F
Department of Medical Malacology, Theodor Bilharz Research Institute, Cairo, Egypt.
Roushdy
M
M
Ibrahim
A
A
El-Hommossany
K
K
Shiff
C
C
D000818:Animals; D001700:Biomphalaria / Q000469:parasitology; D002498:Centrifugation / Q000379:methods; D004534:Egypt; D005374:Filtration; D051379:Mice; D009391:Nephelometry and Turbidimetry / Q000379:methods; D012547:Schistosoma / Q000302:isolation & purification*; D012550:Schistosoma mansoni / Q000302:isolation & purification*; D012555:Schistosomiasis mansoni / Q000635:transmission*; D014867:Water / Q000469:parasitology*
D014867:Water