Glutathione S-transferase a major allergen of the house dust mite, Dermatophagoides pteronyssinus.
Recently, we cloned a new Dermatophagoides pteronyssinus allergen homologous with glutathione S-transferase. IgE radioimmunoassays against the Escherichia coli lysate of the recombinant clone reveal that 40% of mite allergic subjects recognize the mite glutathione S-transferase allergen. Of these sera, greater that 50% have reactivity with the recombinant mite glutathione S-transferase that is greater than 10 times the result observed with a normal control. Immunoblotting studies with sera from patients that recognize the recombinant protein reveal IgE binding to a 26-kDa protein on immunoblots of reduced mite protein extracts. The 26-kDa IgE-binding band observed on immunoblots of reduced mite proteins, corresponding to the cloned protein, is a separate and unique allergen from the 25-kDa Der p I as the apparent electrophoretic molecular mass of Der p I shifts from 25 to 30 kDa under conditions of reduction.
O'Neill
G M
GM
Kolling Institute of Medical Research, Royal North Shore Hospital, St. Leonards, N.S.W., Australia.
Donovan
G R
GR
Baldo
B A
BA
D000485:Allergens / Q000032:analysis* / Q000737:chemistry; D000818:Animals; D039741:Antigens, Dermatophagoides; D001666:Binding Sites, Antibody; D015153:Blotting, Western; D004391:Dust / Q000009:adverse effects; D005982:Glutathione Transferase / Q000737:chemistry / Q000276:immunology*; D006023:Glycoproteins / Q000032:analysis* / Q000737:chemistry; D006801:Humans; D007073:Immunoglobulin E / Q000737:chemistry; D008925:Mites / Q000201:enzymology / Q000276:immunology*; D008970:Molecular Weight; D011994:Recombinant Proteins / Q000737:chemistry / Q000276:immunology
D000485:Allergens; D039741:Antigens, Dermatophagoides; D004391:Dust; D006023:Glycoproteins; D011994:Recombinant Proteins; D007073:Immunoglobulin E; D005982:Glutathione Transferase