Evaluation of CMV Brite kit for detection of cytomegalovirus pp65 antigenemia in peripheral blood leukocytes by immunofluorescence.
The CMV Brite antigenemia kit was compared with culture and an established cytomegalovirus pp65 antigenemia assay (CMV AG). Of 300 clinical specimens tested, 92 were positive by CMV Brite, 83 were positive by CMV AG, and 34 were positive by culture. Discrepancies could be attributed to anticytomegalovirus therapy or low-level antigenemia.
Landry
M L
ML
Department of Laboratory Medicine, Yale University School of Medicine, New Haven, Connecticut 06510 USA.
Ferguson
D
D
Stevens-Ayers
T
T
de Jonge
M W
MW
Boeckh
M
M
D000956:Antigens, Viral / Q000097:blood*; D003587:Cytomegalovirus / Q000276:immunology*; D003586:Cytomegalovirus Infections / Q000175:diagnosis* / Q000821:virology; D003951:Diagnostic Errors; D005069:Evaluation Studies as Topic; D005455:Fluorescent Antibody Technique / Q000379:methods*; D006801:Humans; D010750:Phosphoproteins / Q000097:blood / Q000276:immunology; D014763:Viral Matrix Proteins / Q000097:blood / Q000276:immunology; D014766:Viremia / Q000175:diagnosis* / Q000821:virology; D014773:Virology / Q000379:methods*
D000956:Antigens, Viral; D010750:Phosphoproteins; D014763:Viral Matrix Proteins; C065418:cytomegalovirus matrix protein 65kDa