[The influence of local anesthetics on human leukocyte functions studied by micro whole blood collection and flowcytometry].
The influence of local anesthetics (pure bupivacaine and lidocaine with no preservative) on human leukocyte functions was examined. (a) The effect of bupivacaine on the phagocytosis of granulocyte was studied by bioassay. (b) The effect of lidocaine on the appearance of iC3b receptor (CR3) of granulocyte and monocyte (which is an important cell-adhesion-factor) was examined using flowcytometry. (c) The influence of lidocaine on phagocytosis of granulocyte and monocyte and on respiratory burst of granulocyte was examined using flowcytometry. (d) The influence of lidocaine on phagocytosis and that on respiratory burst were compared. These studies revealed that both phagocytosis and respiratory burst were inhibited by lidocaine, and the inhibition of respiratory burst was stronger than the inhibition of phagocytosis by local anesthetics' immunosuppressive effects. It was concluded that the balance of immunosuppressive action due to antimicrobial action and bactericidal ability of local anesthetics determined the occurrence of local bacterial infection.
Okuno
S
S
Department of Anesthesiology, Juntendo University, Tokyo, Japan.
Noda
H
H
Kugimiya
T
T
Saionji
K
K
D000779:Anesthetics, Local / Q000494:pharmacology*; D001770:Blood Bactericidal Activity; D001800:Blood Specimen Collection; D002045:Bupivacaine / Q000494:pharmacology*; D005434:Flow Cytometry; D006098:Granulocytes / Q000276:immunology; D006801:Humans; D066298:In Vitro Techniques; D007962:Leukocytes / Q000187:drug effects* / Q000502:physiology; D008012:Lidocaine / Q000494:pharmacology*; D009000:Monocytes / Q000276:immunology; D010587:Phagocytosis / Q000187:drug effects*; D016897:Respiratory Burst / Q000187:drug effects
D000779:Anesthetics, Local; D008012:Lidocaine; D002045:Bupivacaine