title: Tumor necrosis factor production by human T-cells stimulated with bacterial superantigens.
abstract: Tumor necrosis factor (TNF) production from T-cells stimulated with superantigenic exotoxins, staphylococcal enterotoxin B and streptococcal pyrogenic exotoxin A was investigated in the presence of cells bearing distinct isotypes of HLA class II molecules. The main T-cell subset for TNF production was investigated in parallel. Similarly high levels of TNF production were induced upon stimulation with the toxins in the presence of DR+ or DQ+ cells, but only marginal levels of TNF production were induced in the presence of DP+ cells. Although both CD4+ T-cells and CD8+ T-cells produced TNF-alpha and TNF-beta in response to toxin stimulation in the presence of HLA class II+ cells, the former T-cell subset was the major source of producers of TNF-alpha and TNF-beta.
authors.affiliation: Department of Microbiology and Immunology, Tokyo Women's Medical College, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Imanishi
authors.forename: K
authors.initials: K
authors.lastname: Inada
authors.forename: H
authors.initials: H
authors.lastname: Akatsuka
authors.forename: Y
authors.initials: Y
authors.lastname: Gu
authors.forename: H
authors.initials: H
authors.lastname: Igarashi
authors.forename: T
authors.initials: T
authors.lastname: Uchiyama
mesh_terms: D001426:Bacterial Proteins; D004768:Enterotoxins / Q000494:pharmacology*; D005098:Exotoxins / Q000494:pharmacology*; D006680:HLA Antigens / Q000276:immunology; D006801:Humans; D066298:In Vitro Techniques; D007376:Interleukin-2 / Q000096:biosynthesis; D008233:Lymphotoxin-alpha / Q000096:biosynthesis; D008565:Membrane Proteins; D013211:Staphylococcus aureus / Q000276:immunology*; D013297:Streptococcus pyogenes / Q000276:immunology*; D018089:Superantigens / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*
chemical_list: D001426:Bacterial Proteins; D004768:Enterotoxins; D005098:Exotoxins; D006680:HLA Antigens; D007376:Interleukin-2; D008233:Lymphotoxin-alpha; D008565:Membrane Proteins; C117980:SpeA protein, Streptococcus pyogenes; D018089:Superantigens; D014409:Tumor Necrosis Factor-alpha; C027951:erythrogenic toxin; C031818:enterotoxin B, staphylococcal