Triiodothyronine regulates beta 3-adrenoceptor expression in 3T3-F442A differentiating adipocytes.
The effect of thyroid hormones on the beta 3-adrenoceptor expression was studied in the preadipose 3T3-F442A cell line. As assessed by molecular and pharmacological analyses, triiodothyronine addition to differentiating 3T3-F442A cells caused a 2.3-fold increase in beta 3-adrenoceptor mRNA levels, which was correlated with a parallel induction of beta 3-adrenoceptor number and of beta 3-adrenoceptor coupling to the adenylate cyclase system. Nuclear transcription experiments showed that triiodothyronine did not significantly alter the transcription rate of the beta 3-adrenoceptor gene. By contrast, the hormone increased by 36% the half-life of beta 3-adrenoceptor mRNA. Triiodothyronine exhibited a discrete effect on beta 3-adrenoceptor expression when added to mature 3T3-F442A adipocytes. This study indicates that thyroid hormones exert a differentiation-dependent and post-transcriptional regulation of beta 3-adrenoceptor expression in adipocytes.
el Hadri
K
K
Institut National de la Santé et de la Recherche Médicale U282, Hôpital Henri Mondor, Créteil, France.
Pairault
J
J
Fève
B
B
D016475:3T3 Cells; D000262:Adenylyl Cyclases / Q000378:metabolism; D017667:Adipocytes / Q000166:cytology / Q000378:metabolism*; D000319:Adrenergic beta-Antagonists / Q000494:pharmacology; D000818:Animals; D001483:Base Sequence; D002454:Cell Differentiation; D002467:Cell Nucleus / Q000378:metabolism; D005576:Colforsin / Q000494:pharmacology; D017931:DNA Primers; D003609:Dactinomycin / Q000494:pharmacology; D015870:Gene Expression / Q000187:drug effects*; D005993:Glycerolphosphate Dehydrogenase / Q000096:biosynthesis / Q000378:metabolism; D016244:Guanosine 5'-O-(3-Thiotriphosphate) / Q000494:pharmacology; D007545:Isoproterenol / Q000494:pharmacology; D007700:Kinetics; D051379:Mice; D008969:Molecular Sequence Data; D011412:Propanolamines / Q000494:pharmacology; D014176:Protein Biosynthesis; D012333:RNA, Messenger; D011943:Receptors, Adrenergic, beta / Q000096:biosynthesis* / Q000187:drug effects / Q000502:physiology; D022702:Receptors, Adrenergic, beta-3; D011994:Recombinant Proteins / Q000096:biosynthesis; D014158:Transcription, Genetic / Q000187:drug effects*; D014162:Transfection; D014284:Triiodothyronine / Q000494:pharmacology*
D000319:Adrenergic beta-Antagonists; D017931:DNA Primers; D011412:Propanolamines; D012333:RNA, Messenger; D011943:Receptors, Adrenergic, beta; D022702:Receptors, Adrenergic, beta-3; D011994:Recombinant Proteins; D014284:Triiodothyronine; D003609:Dactinomycin; D005576:Colforsin; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D005993:Glycerolphosphate Dehydrogenase; D000262:Adenylyl Cyclases; D007545:Isoproterenol; C033394:CGP 12177