Destabilization of simple repetitive DNA sequences by transcription in yeast.
Simple repetitive DNA sequences in the eukaryotic genome frequently alter in length. In wild-type strains, we find that transcription through a repetitive poly GT tract destabilizes the tract four- to ninefold. In mismatch repair-deficient yeast strains, simple repeats are very unstable. High levels of transcription in such strains destabilize repetitive tracts an additional two- to threefold.
Wierdl
M
M
Department of Biology, University of North Carolina, Chapel Hill 27599-3280, USA.
Greene
C N
CN
Datta
A
A
Jinks-Robertson
S
S
Petes
T D
TD
D004249:DNA Damage; D004260:DNA Repair; D004271:DNA, Fungal / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D017422:Sequence Analysis, DNA; D014158:Transcription, Genetic
D004271:DNA, Fungal