title: Sequencing and analysis of bacterial genomes.
abstract: The complete sequences of two small bacterial genomes have recently become available, and those of several more species should follow within the next two years. Sequence comparisons show that the most bacterial proteins are highly conserved in evolution, allowing predictions to be made about the functions of most products of an uncharacterized genome. Bacterial genomes differ vastly in their gene repertoires. Although genes for components of the translation and transcription machinery, and for molecular chaperones, are typically maintained, many regulatory and metabolic systems are absent in bacteria with small genomes. Mycoplasma genitalium, with the smallest known genome of any cellular life form, lacks virtually all known regulatory genes, and its gene expression may be regulated differently than in other bacteria. Genome organization is evolutionarily labile: extensive gene shuffling leaves only very few conserved gene arrays in distantly related bacteria.
authors.affiliation: National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, Maryland 20894, USA.
authors.forename: E V
authors.initials: EV
authors.lastname: Koonin
authors.forename: A R
authors.initials: AR
authors.lastname: Mushegian
authors.forename: K E
authors.initials: KE
authors.lastname: Rudd
mesh_terms: D000595:Amino Acid Sequence; D001330:Electronic Data Processing; D005057:Eukaryotic Cells; D016680:Genome, Bacterial; D008969:Molecular Sequence Data; D017421:Sequence Analysis