An investigation of the interaction between alcohol and fibroblasts in wound healing.
This study investigated the effect of clinical concentrations of alcohol on fibroblast function (proliferation and ECM synthesis) in vitro. Basal and TGF-beta-induced collagen synthesis was assayed in confluent cultures in serum-free medium at 48 h with a commercial collagen assay system. At concentrations of alcohol > 5%, fibroblast proliferation was significantly inhibited. Although noninhibitory, subclinical concentrations of alcohol failed to inhibit basal collagen synthesis (P > 0.1), they significantly decreased TGF-beta-induced collagen synthesis (P < 0.03). These data support the notion that the local, as well as the systemic, effects of alcohol are important in mediating delayed healing in alcoholic patients.
Stephens
P
P
Department of Oral Surgery, Medicine and Pathology, University of Wales College of Medicine, Cardiff, UK.
al-Khateeb
T
T
Davies
K J
KJ
Shepherd
J P
JP
Thomas
D W
DW
D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis; D016895:Culture Media, Serum-Free; D000431:Ethanol / Q000633:toxicity*; D016326:Extracellular Matrix Proteins / Q000096:biosynthesis; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D006801:Humans; D016212:Transforming Growth Factor beta / Q000037:antagonists & inhibitors / Q000494:pharmacology; D014945:Wound Healing / Q000187:drug effects*
D016895:Culture Media, Serum-Free; D016326:Extracellular Matrix Proteins; D016212:Transforming Growth Factor beta; D000431:Ethanol; D003094:Collagen