title: Anti-gamma delta T cell antibody blocks the induction and maintenance of oral tolerance to ovalbumin in mice.
abstract: The oral administration of antigens is one of the means of inducing tolerance in adult mammals. In this report, the role of gamma delta T cells in the induction and maintenance of orally-induced tolerance to ovalbumin was investigated. The injection of a monoclonal anti-gamma delta T cell monoclonal antibody blocked the induction of oral tolerance, because the secondary immune responses to ovalbumin in these animals were comparable to the corresponding responses in ovalbumin-immunized control mice. Furthermore, depletion of gamma delta T cells either in vivo or in vitro abolished already established oral-tolerance. The fact that the state of tolerance could be adoptively transferred to naive recipients by CD3+ alpha beta- gamma delta + spleen cells from tolerant mice. These results suggest that systemic oral tolerance is induced and actively maintained by mechanisms involving gamma delta T cells.
authors.affiliation: Department of Immunology, University of São Paulo, Brazil.
authors.forename: J
authors.initials: J
authors.lastname: Mengel
authors.forename: F
authors.initials: F
authors.lastname: Cardillo
authors.forename: L S
authors.initials: LS
authors.lastname: Aroeira
authors.forename: O
authors.initials: O
authors.lastname: Williams
authors.forename: M
authors.initials: M
authors.lastname: Russo
authors.forename: N M
authors.initials: NM
authors.lastname: Vaz
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage / Q000494:pharmacology*; D017252:CD3 Complex / Q000276:immunology; D002478:Cells, Cultured; D007108:Immune Tolerance; D016219:Immunotherapy, Adoptive; D008214:Lymphocytes / Q000276:immunology*; D051379:Mice; D008811:Mice, Inbred DBA; D010047:Ovalbumin / Q000008:administration & dosage* / Q000276:immunology*; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000276:immunology*; D013154:Spleen / Q000166:cytology / Q000637:transplantation
chemical_list: D000911:Antibodies, Monoclonal; D017252:CD3 Complex; D016692:Receptors, Antigen, T-Cell, gamma-delta; D010047:Ovalbumin