Nitric oxide synthase-containing neurons in the hippocampus are preserved in trimethyltin intoxication.
We studied the effects of trimethyltin (TMT) (9 mg/kg, p.o.) on the nitric oxide synthase (NOS)-containing neurons in the rat hippocampus by NADPH-diaphorase histochemistry and a biochemical assay of NOS activity. TMT exposure caused the typical behavioral changes and a loss of the CA3/4 pyramidal cells, which were NADPH diaphorase-negative. The scattered interneurons and the CA1 pyramidal cells, which were NADPH diaphorase-positive, were spared. Hippocampal NOS activity showed no reduction in the TMT-treated rats compared with the controls. These results provide evidence of the preservation of the NOS-containing neurons in TMT intoxication.
Ikeda
M
M
Department of Physiological Chemistry, Graduate School, Tokyo Medical and Dental University, Japan. ikeda.cell@dent.tmd.ac.jp
Kanai
H
H
Akaike
M
M
Tsutsumi
S
S
Sadamatsu
M
M
Masui
A
A
Kato
N
N
D000818:Animals; D006624:Hippocampus / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D006651:Histocytochemistry; D007395:Interneurons / Q000187:drug effects / Q000201:enzymology; D008297:Male; D009252:NADPH Dehydrogenase / Q000032:analysis*; D009474:Neurons / Q000187:drug effects* / Q000201:enzymology; D019001:Nitric Oxide Synthase / Q000032:analysis*; D017966:Pyramidal Cells / Q000187:drug effects / Q000201:enzymology; D051381:Rats; D017207:Rats, Sprague-Dawley; D014298:Trimethyltin Compounds / Q000506:poisoning*
D014298:Trimethyltin Compounds; C046488:trimethyltin; D019001:Nitric Oxide Synthase; D009252:NADPH Dehydrogenase