title: Regulation of steady-state concentrations of messenger ribonucleic acid encoding prostaglandin F2 alpha receptor in ovine corpus luteum.
abstract: To investigate the regulation of ovine luteal receptors for prostaglandin F2 alpha (PGF2 alpha), reverse transcription-polymerase chain reaction was used to produce a 284-bp partial cDNA that was 98% identical to that reported for the bovine PGF2 alpha receptor (PGF2 alpha-R). In situ hybridization localized mRNA for PGF2 alpha-R specifically to large luteal cells. In experiment 1, pools of luteal tissue (n = 4/day) collected from ewes on Days 3, 6, 9, 12, and 15 of the estrous cycle were analyzed for mRNA encoding PGF2 alpha-R. There was no difference in mean steady-state concentrations of mRNA encoding PGF2 alpha-R among any of the days studied (range = 2.3 +/- 0.3 to 3.5 +/- 0.7 fmol PGF2 alpha-R mRNA/ microgram poly[A]+ RNA as assessed by slot-blot hybridization). In experiment 2, ewes on Day 11 or Day 12 of the estrous cycle were administered PGF2 alpha, and corpora lutea were collected 4, 12, or 24 h later (n = 4-5 per time point). Nontreated (n = 4) or saline-treated (n = 4) ewes served as controls. Luteal concentrations of mRNA encoding PGF2 alpha-R were decreased (p < 0.05) at 4, 12, and 24 h after injection of PGF2 alpha. In experiment 3, ewes (midluteal phase) were administered saline, PGF2 alpha, phorbol 12-myristate 13-acetate (PMA), or LH via ovarian arterial injection, and luteal tissue was collected 0, 4, 12, or 24 h later (n = 3-4 per treatment per time). Steady-state concentrations of mRNA encoding PGF2 alpha-R were decreased (p < 0.05) by PGF2 alpha and PMA treatment (4 and 12 h) but were increased (p < 0.05) at 24 h after LH treatment. In summary, 1) mRNA encoding PGF2 alpha-R was localized to large luteal cells; 2) concentrations of mRNA encoding PGF2 alpha-R did not vary during the estrous cycle; 3) treatment with PGF2 alpha or PMA to activate protein kinase C decreased concentrations of PGF2 alpha-R mRNA within 4 h of treatment; and 4) administration LH increased concentrations of mRNA encoding PGF2 alpha-R 24 h following injection.
authors.affiliation: Animal reproduction and biotechnology Laboratory, Colorado State University, Fort Collins 80523-1683, USA.
authors.forename: J L
authors.initials: JL
authors.lastname: Juengel
authors.forename: M C
authors.initials: MC
authors.lastname: Wiltbank
authors.forename: B M
authors.initials: BM
authors.lastname: Meberg
authors.forename: G D
authors.initials: GD
authors.lastname: Niswender
mesh_terms: D000818:Animals; D003338:Corpus Luteum / Q000378:metabolism*; D015237:Dinoprost / Q000494:pharmacology; D005260:Female; D005786:Gene Expression Regulation; D007986:Luteinizing Hormone / Q000494:pharmacology; D016133:Polymerase Chain Reaction; D011374:Progesterone / Q000097:blood; D012333:RNA, Messenger / Q000378:metabolism*; D012194:RNA-Directed DNA Polymerase; D011982:Receptors, Prostaglandin / Q000235:genetics*; D012756:Sheep; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: D012333:RNA, Messenger; D011982:Receptors, Prostaglandin; C034671:prostaglandin F2alpha receptor; D011374:Progesterone; D007986:Luteinizing Hormone; D015237:Dinoprost; D012194:RNA-Directed DNA Polymerase; D013755:Tetradecanoylphorbol Acetate