title: An improved method for in vitro susceptibility testing of Trichomonas vaginalis.
abstract: A protocol is presented for in vitro susceptibility testing of Trichomonas vaginalis. A 100 ml culture of the microorganisms is prepared for inoculation into antibiotic dilutions and controls by centrifugation, washing with 10 mM HEPES (pH 6.2) plus 1.5 x 10(-1) M NaCl, a second centrifugation and a resuspension in the HEPES-saline buffer. Inclusion of the gelling agent carrageenan in the culture medium permits an ease of harvesting the trichomonads and a reproducible initial cell density of 1-4 x 10(4) cells per ml. Following inoculation, tubes with antibiotic dilutions and controls are incubated anaerobically at 35 degrees C for 48 h, which corresponds to late exponential phase. Inclusion of a negative control helps determine Minimum Lethal Concentration (MLC) values.
authors.affiliation: Department of Microbiology and Immunology, Philadelphia College of Osteopathic Medicine, PA, USA.
authors.forename: B J
authors.initials: BJ
authors.lastname: Bromke
authors.forename: M C
authors.initials: MC
authors.lastname: Furiga
authors.forename: R C
authors.initials: RC
authors.lastname: Hendershot
authors.forename: M
authors.initials: M
authors.lastname: McGinn
mesh_terms: D000818:Animals; D000994:Antitrichomonal Agents / Q000633:toxicity*; D004351:Drug Resistance; D005260:Female; D006801:Humans; D008795:Metronidazole / Q000633:toxicity*; D010274:Parasitology / Q000379:methods*; D014247:Trichomonas Vaginitis / Q000469:parasitology; D014246:Trichomonas vaginalis / Q000187:drug effects* / Q000302:isolation & purification
chemical_list: D000994:Antitrichomonal Agents; D008795:Metronidazole