title: A novel kinesin-like protein with a calmodulin-binding domain.
abstract: Calcium regulates diverse developmental processes in plants through the action of calmodulin. A cDNA expression library from developing anthers of tobacco was screened with 35S-labeled calmodulin to isolate cDNAs encoding calmodulin-binding proteins. Among several clones isolated, a kinesin-like gene (TCK1) that encodes a calmodulin-binding kinesin-like protein was obtained. The TCK1 cDNA encodes a protein with 1265 amino acid residues. Its structural features are very similar to those of known kinesin heavy chains and kinesin-like proteins from plants and animals, with one distinct exception. Unlike other known kinesin-like proteins, TCK1 contains a calmodulin-binding domain which distinguishes it from all other known kinesin genes. Escherichia coli-expressed TCK1 binds calmodulin in a Ca(2+)-dependent manner. In addition to the presence of a calmodulin-binding domain at the carboxyl terminal, it also has a leucine zipper motif in the stalk region. The amino acid sequence at the carboxyl terminal of TCK1 has striking homology with the mechanochemical motor domain of kinesins. The motor domain has ATPase activity that is stimulated by microtubules. Southern blot analysis revealed that TCK1 is coded by a single gene. Expression studies indicated that TCK1 is expressed in all of the tissues tested. Its expression is highest in the stigma and anther, especially during the early stages of anther development. Our results suggest that Ca2+/calmodulin may play an important role in the function of this microtubule-associated motor protein and may be involved in the regulation of microtubule-based intracellular transport.
authors.affiliation: Department of Horticulture, Washington State University, Pullman 99164-6414, USA.
authors.forename: W
authors.initials: W
authors.lastname: Wang
authors.forename: D
authors.initials: D
authors.lastname: Takezawa
authors.forename: S B
authors.initials: SB
authors.lastname: Narasimhulu
authors.forename: A S
authors.initials: AS
authors.lastname: Reddy
authors.forename: B W
authors.initials: BW
authors.lastname: Poovaiah
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002118:Calcium / Q000378:metabolism; D002147:Calmodulin / Q000378:metabolism*; D002148:Calmodulin-Binding Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D018076:DNA, Complementary; D004926:Escherichia coli / Q000235:genetics; D016547:Kinesins / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D010947:Plants, Toxic; D011485:Protein Binding; D017433:Protein Structure, Secondary; D017386:Sequence Homology, Amino Acid; D014026:Nicotiana / Q000235:genetics*
chemical_list: D002147:Calmodulin; D002148:Calmodulin-Binding Proteins; D018076:DNA, Complementary; D010940:Plant Proteins; C100819:TCK1 protein, Nicotiana tabacum; D000251:Adenosine Triphosphatases; D016547:Kinesins; D002118:Calcium