title: A model phage display subtraction method with potential for analysis of differential gene expression.
abstract: In order to establish a subtractive procedure that makes it possible to enrich selectively phage displayed antibodies directed against proteins constituting a difference between two populations of cells, a competitive selection strategy utilising two solid phases was developed and tested. Antibodies recognising a defined difference between two otherwise identical protein mixtures were isolated and their specificity confirmed. To test further the efficacy of selection inhibition during the competitive selections, selections towards a total cell extract were performed with and without competition from the same extract. An analysis of the resulting phage antibodies confirmed the subtractive nature of the system described.
authors.affiliation: Institute of Molecular and Structural Biology, Biostructural Chemistry, University of Aarhus, Denmark. groen@biobase.dk
authors.forename: B
authors.initials: B
authors.lastname: Stausbøl-Grøn
authors.forename: T
authors.initials: T
authors.lastname: Wind
authors.forename: S
authors.initials: S
authors.lastname: Kjaer
authors.forename: L
authors.initials: L
authors.lastname: Kahns
authors.forename: N J
authors.initials: NJ
authors.lastname: Hansen
authors.forename: P
authors.initials: P
authors.lastname: Kristensen
authors.forename: B F
authors.initials: BF
authors.lastname: Clark
mesh_terms: D000818:Animals; D000906:Antibodies; D000918:Antibody Specificity; D000941:Antigens / Q000032:analysis*; D001435:Bacteriophages; D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay; D015870:Gene Expression; D006801:Humans; D007135:Immunoglobulin Variable Region; D007256:Information Systems; D008545:Melanoma; D014176:Protein Biosynthesis; D011506:Proteins / Q000032:analysis*; D014407:Tumor Cells, Cultured
chemical_list: D000906:Antibodies; D000941:Antigens; D007135:Immunoglobulin Variable Region; D011506:Proteins