title: Determination of the 5' and 3' terminal noncoding sequences of the bi-segmented genome of the avibirnavirus infectious bursal disease virus.
abstract: Terminal sequences of the bi-segmented dsRNA genome of 3 different strains of infectious bursal disease virus (IBDV) were analyzed by the rapid amplification of cDNA 5' ends (5'RACE) procedure. Both segments are 85% homologous in a 32-nucleotide sequence comprising the 5' end, whereas the 3' end has a conserved pentamer. Comparison to published terminal sequences of other IBDV strains revealed high conservation between the two segments but more serotype-specific nucleotide changes (5 on segment A and 3 on segment B) in the 5' noncoding region compared to the 3' noncoding region (none on segment A and 1 on segment B).
authors.affiliation: Department of Pathology and Microbiology, Atlantic Veterinary College, University of Prince Edward Island, Charlottetown, Canada.
authors.forename: F S
authors.initials: FS
authors.lastname: Kibenge
authors.forename: M M
authors.initials: MM
authors.lastname: Nagarajan
authors.forename: B
authors.initials: B
authors.lastname: Qian
mesh_terms: D000818:Animals; D001483:Base Sequence; D018175:Birnaviridae Infections / Q000662:veterinary / Q000821:virology; D002645:Chickens; D004279:DNA, Viral; D016679:Genome, Viral; D007243:Infectious bursal disease virus / Q000235:genetics*; D007438:Introns; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D011201:Poultry Diseases / Q000821:virology; D012367:RNA, Viral; D014764:Viral Proteins / Q000235:genetics
chemical_list: D004279:DNA, Viral; D012367:RNA, Viral; D014764:Viral Proteins