Oxygen-dependent leishmanicidal activity of stimulated macrophages.
Peritoneal macrophages pretreated with different stimulants were analysed and compared with their respective controls for their ability to kill intracellular pathogenic L. donovani, (MHOM/IN/1983/AG83) an isolate from Indian subcontinent. Stimulation of macrophages by zymosan showed a higher microbicidal activity as compared to that by PMA. A correlation between microbicidal activity of the macrophages and the parameters related to respiratory burst activity such as liberation of O2-, production of H2O2 and consumption of O2 was sought. All the parameters showed a decrease in case of infected macrophages in comparison to those of the non-infected ones. Thus, it is possible that the impairment of macrophage activation by intracellular Leishmania contributes to their survival in the toxic environment of the host.
Chakraborty
R
R
Biomembrane Division, Indian Institute of Chemical Biology, Calcutta, India.
Mukherjee
S
S
Basu
M K
MK
D000818:Animals; D002478:Cells, Cultured; D006861:Hydrogen Peroxide / Q000378:metabolism; D007893:Leishmania donovani; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000469:parasitology* / Q000502:physiology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010101:Oxygen Consumption; D010587:Phagocytosis / Q000187:drug effects; D016897:Respiratory Burst; D013481:Superoxides / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D015054:Zymosan / Q000494:pharmacology
D013481:Superoxides; D015054:Zymosan; D006861:Hydrogen Peroxide; D013755:Tetradecanoylphorbol Acetate