title: Low affinity purinergic receptor modulates the response of rat submandibular glands to carbachol and substance P.
abstract: The effect of extracellular ATP on the intracellular calcium concentration ([Ca2+]i) in rat submandibular glands was tested. The dose-response curve for ATP was biphasic with a first increase in the 1-30 microM concentration range and a further increase at concentrations higher than 100 microM. Among ATP analogs, only benzoyl-ATP stimulated the low affinity component. ATP tau S blocked this response. All the other analogs tested reproduced the high-affinity low capacity response. Magnesium and Coomassie blue selectively blocked the low affinity component. High concentrations of ATP blocked the increase of the intracellular calcium concentration [Ca2+]i in response to 100 microM carbachol. By itself, substance P (100 pM-1 microM) increased the [Ca2+]i. One mM ATP potentiated the response to concentrations of substance P higher than 10 nM. This potentiation was reversed by extracellular magnesium. Carbachol 100 microM and substance P (100 pM-1 microM) increased the release of inositol trisphosphate (IP3) from polyphosphoinositides (polyPI). Activation of the low affinity ATP receptors did not activate the polyPI-specific phospholipase C but inhibited its activation by 100 microM carbachol (-50%) and by 100 nM substance P (-60% at 1 nM substance P and -40% at 100 nM substance P). Substance P induced a strong homologous desensitization: a preincubation with 1 nM substance P nearly completely abolished the response to 1 microM substance P. When the cells were exposed to ATP before the second addition of substance P, the purinergic agonist partially restored the response to the tachykinin without totally reversing the desensitization. It is concluded that two types of purinergic receptors coexist in rat submandibular glands; a high-affinity, low capacity receptor which remains pharmacologically and functionally undefined and a low affinity site, high capacity receptor of the P2z type coupled to a non-selective cation channel. The occupancy of these low affinity sites blocks the increase of the [Ca2+]i in response to a muscarinic agonist and the activation of polyPI-specific phospholipase C by carbachol and substance P. It potentiates the effect of high concentrations of substance P on the [Ca2+]i.
authors.affiliation: Laboratoire de Biochimie générale et humaine, Université libre de Bruxelles, Belgium.
authors.forename: M
authors.initials: M
authors.lastname: Métioui
authors.forename: H
authors.initials: H
authors.lastname: Amsallem
authors.forename: E
authors.initials: E
authors.lastname: Alzola
authors.forename: N
authors.initials: N
authors.lastname: Chaib
authors.forename: A
authors.initials: A
authors.lastname: Elyamani
authors.forename: A
authors.initials: A
authors.lastname: Moran
authors.forename: A
authors.initials: A
authors.lastname: Marino
authors.forename: J P
authors.initials: JP
authors.lastname: Dehaye
mesh_terms: D000255:Adenosine Triphosphate / Q000031:analogs & derivatives / Q000494:pharmacology*; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002217:Carbachol / Q000494:pharmacology*; D005456:Fluorescent Dyes / Q000378:metabolism; D016257:Fura-2 / Q000378:metabolism; D066298:In Vitro Techniques; D007424:Intracellular Fluid / Q000737:chemistry / Q000378:metabolism; D008274:Magnesium / Q000378:metabolism / Q000494:pharmacology; D008297:Male; D058913:Purinergic Agonists; D051381:Rats; D017208:Rats, Wistar; D011983:Receptors, Purinergic / Q000378:metabolism*; D012394:Rosaniline Dyes / Q000494:pharmacology; D013363:Submandibular Gland / Q000187:drug effects* / Q000378:metabolism; D013373:Substance P / Q000494:pharmacology*; D013729:Terpenes / Q000494:pharmacology; D019284:Thapsigargin
chemical_list: D005456:Fluorescent Dyes; D058913:Purinergic Agonists; D011983:Receptors, Purinergic; D012394:Rosaniline Dyes; D013729:Terpenes; D013373:Substance P; D019284:Thapsigargin; C048139:Coomassie blue; D000255:Adenosine Triphosphate; D002217:Carbachol; D008274:Magnesium; D002118:Calcium; D016257:Fura-2