The mRNA for an ETR1 homologue in tomato is constitutively expressed in vegetative and reproductive tissues.
Dominant mutations in the Arabidopsis ETR1 gene block the ethylene signal transduction pathway. The ETR1 gene has been cloned and sequenced. Using the ETR1 cDNA as a probe, we identified a cDNA homologue (eTAE1) from tomato. eTAE1 contains an open reading frame encoding a polypeptide of 754 amino acid residues. The nucleic acid sequence for the coding sequence in eTAE1 is 74% identical to that for ETR1, and the deduced amino acid sequence is 81% identical and 90% similar. Genomic Southern blot analysis indicates that three or more ETR1 homologues exist in tomato. RNA blots show that eTAE1 mRNA is constitutively expressed in all the tissues examined, and its accumulation in leaf abscission zones was unaffected by ethylene, silver ions (an inhibitor of ethylene action) or auxin.
Zhou
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Plant Molecular Biology Laboratory, USDA/ARS, Beltsville, MD 20705, USA.
Kalaitzís
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Mattoo
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Tucker
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D000040:Abscisic Acid / Q000502:physiology; D000595:Amino Acid Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D018076:DNA, Complementary; D018506:Gene Expression Regulation, Plant / Q000502:physiology; D018551:Solanum lycopersicum / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010940:Plant Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D011956:Receptors, Cell Surface; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D010940:Plant Proteins; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; C100793:TAE1 protein, Lycopersicon esculentum; C083750:ethylene receptors, plant; D000040:Abscisic Acid