title: Effect of radiation on red cell membrane and intracellular oxidative defense systems.
abstract: Ionizing radiation is currently used for prevention of transfusion associated graft versus host disease (TAGVHD). As radiation damage is associated with the production of activated oxygen species, the aim of this study was to observe the immediate effect of ionizing radiation on red cell membrane and intracellular oxidative defense systems. Neonatal and iron deficiency (IDA) cells, known for their increased sensitivity to oxidative stress, were chosen and compared with normal cells. Irradiation was performed in doses of 1500 cGy, 3000 cGy and 5000 cGy. GSH and methemoglobin levels and the activity of different antioxidant enzymes, measured under optimal in vitro conditions, were preserved in all cells after irradiation. Only radiation at the highest does of 5000 cGy, caused significant potassium leakage in neonatal cells and insignificant increase in IDA cells. Thus, cells with increased sensitivity to oxidative stress are more susceptible to damage by ionizing radiation than normal cells.
authors.affiliation: Dr. Joseph Kaufmann Hematology Laboratory, Corob Medical Research Center, Faculty of Health Sciences, Ben-Gurion, University of the Negev, Beer-Sheva, Israel.
authors.forename: D
authors.initials: D
authors.lastname: Katz
authors.forename: D
authors.initials: D
authors.lastname: Mazor
authors.forename: A
authors.initials: A
authors.lastname: Dvilansky
authors.forename: N
authors.initials: N
authors.lastname: Meyerstein
mesh_terms: D002374:Catalase / Q000097:blood; D004307:Dose-Response Relationship, Radiation; D004910:Erythrocyte Membrane / Q000378:metabolism / Q000528:radiation effects*; D004912:Erythrocytes / Q000378:metabolism / Q000528:radiation effects*; D005260:Female; D005312:Fetal Blood / Q000528:radiation effects*; D005978:Glutathione / Q000097:blood; D005979:Glutathione Peroxidase / Q000097:blood; D005980:Glutathione Reductase / Q000097:blood; D006086:Graft vs Host Disease / Q000517:prevention & control; D006400:Hematocrit; D006454:Hemoglobins / Q000378:metabolism / Q000528:radiation effects; D006801:Humans; D007231:Infant, Newborn; D000090463:Iron Deficiencies; D008706:Methemoglobin / Q000528:radiation effects; D018384:Oxidative Stress; D010920:Placenta; D011188:Potassium / Q000097:blood; D011247:Pregnancy; D011839:Radiation, Ionizing; D013482:Superoxide Dismutase / Q000097:blood
chemical_list: D006454:Hemoglobins; D008706:Methemoglobin; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase; D005978:Glutathione; D011188:Potassium