Isolation and genetic characterization of pGKL killer-insensitive mutants (iki) from Saccharomyces cerevisiae.
The linear double stranded DNA plasmid pGKL1 encodes the yeast killer toxin complex (Gunge et al., 1981) of which the killing mechanism is not understood. We isolated and characterized eight mutants in Saccharomyces cerevisiae that were insensitive to both the intracellularly expressed 28-kDa killer subunit and the native killer toxin complex. These mutations (iki1 through iki5) were all recessive, and classified into five complementation groups. The iki2 mutation was mapped to a position near the centromere on chromosome XIII. We developed a novel screening system to isolate the DNA fragments complementing the iki mutations from a Saccharomyces gene library, and isolated three DNA fragments that complement the iki1, iki3, and iki4 mutations, respectively.
Kishida
M
M
Mitsubishi Kasei Institute of Life Sciences, Tokyo, Japan.
Tokunaga
M
M
Katayose
Y
Y
Yajima
H
H
Kawamura-Watabe
A
A
Hishinuma
F
F
D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D004271:DNA, Fungal / Q000235:genetics* / Q000302:isolation & purification; D005656:Fungal Proteins / Q000235:genetics / Q000494:pharmacology*; D015723:Gene Library; D005816:Genetic Complementation Test; D055646:Killer Factors, Yeast; D008970:Molecular Weight; D009154:Mutation / Q000235:genetics*; D009183:Mycotoxins / Q000235:genetics / Q000494:pharmacology*; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D029701:Saccharomyces cerevisiae Proteins
D004271:DNA, Fungal; D005656:Fungal Proteins; D055646:Killer Factors, Yeast; D009183:Mycotoxins; D029701:Saccharomyces cerevisiae Proteins; D004247:DNA