Lysis buffer composition dramatically affects extraction of phosphotyrosine-containing proteins.
Ignatoski
K M
KM
Howard Hughes Medical Institute, University of Michigan Medical School, Ann Arbor 48109-0650. kwi@umich. edu
Verderame
M F
MF
D000818:Animals; D002021:Buffers; D004591:Electrophoresis, Polyacrylamide Gel; D019000:Phosphotyrosine / Q000276:immunology*; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D011506:Proteins / Q000302:isolation & purification*; D051381:Rats; D014162:Transfection
D002021:Buffers; D011506:Proteins; D019000:Phosphotyrosine; D011505:Protein-Tyrosine Kinases