The construction and characterization of an effective transpositional system based on IS30.
We constructed an in vivo system to detect transpositional rearrangements induced by the insertion sequence IS30. The transposase protein expressed from the transposase producer plasmids catalyzed rearrangements on different target sequences presented in trans. High yields, up to 83%, of transpositional frequencies were observed. The frequency of rearrangements correlated with the amount of transposase protein produced and the attractivity of the target sequences. Alteration in the frequency of transposition was observed in the recA- E. coli strains JM109 and TG2. Remarkable structural and functional analogy was found with site-specific recombination systems.
Farkas
T
T
Institute for Molecular Genetics, Agricultural Biotechnology Center, Gödöllö, Hungary.
Kiss
J
J
Olasz
F
F
D004254:DNA Nucleotidyltransferases / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism*; D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D015321:Gene Rearrangement; D007544:Isopropyl Thiogalactoside / Q000494:pharmacology; D008957:Models, Genetic; D016366:Open Reading Frames; D010957:Plasmids; D011938:Rec A Recombinases / Q000235:genetics; D011995:Recombination, Genetic; D013045:Species Specificity; D014158:Transcription, Genetic; D019895:Transposases
D004251:DNA Transposable Elements; D007544:Isopropyl Thiogalactoside; D004254:DNA Nucleotidyltransferases; D011938:Rec A Recombinases; D019895:Transposases