Relationship between interferon-gamma, interleukin-4 and IgE production of lymphocytes from hen's egg-sensitive patients.
We measured in vitro interferon-gamma, interleukin-4 and IgE production of peripheral blood mononuclear cells stimulated with ovalbumin. Interferon-gamma in culture supernatants of ovalbumin-stimulated peripheral blood mononuclear cells from hen's egg- sensitive patients with atopic dermatitis was significantly higher than that of healthy children or hen's egg-sensitive patients with immediate symptoms. Furthermore, in patients with atopic dermatitis who were sensitive to hen's egg and patients with immediate symptoms, there was an inverse relationship between interferon-gamma and IgE production (r = -0.535, p <0.05), and significant correlation was found between interleukin-4 and IgE production (r = 0.802, p <0.05). For the above reasons, IgE synthesis of ovalbumin-stimulated peripheral blood mononuclear cells may be suppressed by interferon-gamma in patients with atopic dermatitis. In contrast, in patients with immediate symptoms, interleukin-4 may play a major role in the pathogenesis of increased IgE production.
Kuwabara
N
N
Department of Pediatrics, Gifu University School of Medicine, Japan.
Kondo
N
N
Fukutomi
O
O
Agata
H
H
Orii
T
T
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000494:pharmacology; D002645:Chickens; D002648:Child; D002675:Child, Preschool; D004531:Eggs / Q000009:adverse effects*; D005512:Food Hypersensitivity / Q000276:immunology*; D006801:Humans; D007073:Immunoglobulin E / Q000096:biosynthesis* / Q000276:immunology; D007223:Infant; D007371:Interferon-gamma / Q000096:biosynthesis* / Q000494:pharmacology; D015847:Interleukin-4 / Q000494:pharmacology / Q000502:physiology*; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000378:metabolism*; D010047:Ovalbumin / Q000494:pharmacology; D011852:Radioallergosorbent Test
D000888:Antibodies, Anti-Idiotypic; C026408:anti-IgE antibodies; D015847:Interleukin-4; D007073:Immunoglobulin E; D007371:Interferon-gamma; D010047:Ovalbumin