Effects of sonodynamic and photodynamic treatment on cellular thiol levels.
Exposure of murine leukemia L1210 cells to ultrasound in vitro resulted in cell fragmentation; cells which survived this treatment were 100% viable and showed a transient decrease in intracellular thiol pools (expressed as glutathione equivalents). Depletion of thiol pools became progressively greater with increasing exposure of cells to ultrasound; this was not associated with leakage of thiols into the medium. Biochemically elevating glutathione levels or sonication in the presence of the anti-oxidant trolox offered only a minor degree of protection against sonotoxicity. Photodynamic therapy also resulted in depletion of intracellular thiol levels, and loss of cell viability. In the presence of trolox, both effects were reversed, although elevation of intracellular thiol levels did not protect cell from phototoxicity.
Kessel
D
D
Department of Pharmacology, Wayne State University School of Medicine, Detroit, MI 48201, USA.
Jeffers
R
R
Fowlkes
J B
JB
Cain
C
C
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D002839:Chromans / Q000494:pharmacology; D005456:Fluorescent Dyes; D005978:Glutathione / Q000378:metabolism* / Q000528:radiation effects; D007939:Leukemia L1210 / Q000378:metabolism*; D008027:Light; D008652:Mesoporphyrins / Q000494:pharmacology*; D051379:Mice; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000494:pharmacology*; D013438:Sulfhydryl Compounds / Q000378:metabolism* / Q000528:radiation effects; D014407:Tumor Cells, Cultured; D014410:Tumor Stem Cell Assay; D014465:Ultrasonics
D000975:Antioxidants; D002839:Chromans; D005456:Fluorescent Dyes; D008652:Mesoporphyrins; D017319:Photosensitizing Agents; D013438:Sulfhydryl Compounds; C031468:mesoporphyrin IX; D005978:Glutathione; C010643:6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid