title: Neuropeptide Y gene expression and receptor autoradiography in hypertensive and normotensive rat brain.
abstract: Neurones containing neuropeptide Y (NPY) may participate in central cardiovascular control by tonically influencing barosensitive neurones within the nucleus tractus solitarius. The present study has employed both in situ hybridisation histochemistry and receptor autoradiography, to visualise the expression of prepro-NPY mRNA in the forebrain and to determine the NPY receptor subtype(s) in the brainstem, respectively. Prepro-NPY gene expression was visualised in the hypothalamus, cortex, dentate gyrus and lateral reticular thalamus from age-matched spontaneously hypertensive rats (SHR) and normotensive Don Ryu rats (DRY) and Wistar Kyoto rats (WKY). Quantitative densitometry revealed an increase in the NPY transcript in the arcuate nucleus of SHR rats compared to their normotensive counterparts. Autoradiography using [125I]Bolton-Hunter-NPY (BH-NPY, 15 pM) demonstrated NPY binding sites in the area postrema, the commissural nucleus tractus solitarius (cNTS) and the inferior olivary complex. NPY (1 microM) and peptide YY (1 microM), but not [Leu31,Pro34]NPY (10-100 nM), fully inhibited the binding of [125I]BH-NPY. These results indicate that NPY receptors of the Y2 subtype predominate in the dorsal vagal complex. Unilateral nodose ganglionectomy resulted in a partial loss of NPY binding sites in the commissural NTS, but not the area postrema, suggesting that a proportion of binding sites (Y2 subtype) are present on central vagal terminals. While all three rat strains appear to have the same relative proportions of NPY receptor subtypes in the brainstem, the relevance of the differential NPY gene expression in the arcuate nucleus regarding central cardiovascular control mechanisms and/or the pathogenesis of hypertension remains to be elucidated.
authors.affiliation: Department of Pharmacology, Monash University, Clayton, Vic., Australia.
authors.forename: K J
authors.initials: KJ
authors.lastname: McLean
authors.forename: B
authors.initials: B
authors.lastname: Jarrott
authors.forename: A J
authors.initials: AJ
authors.lastname: Lawrence
mesh_terms: D000818:Animals; D001345:Autoradiography; D001483:Base Sequence; D001921:Brain / Q000378:metabolism*; D015870:Gene Expression; D006973:Hypertension / Q000378:metabolism*; D017403:In Situ Hybridization; D007457:Iodine Radioisotopes; D008297:Male; D008969:Molecular Sequence Data; D009474:Neurons / Q000378:metabolism*; D009478:Neuropeptide Y / Q000096:biosynthesis*; D009620:Nodose Ganglion / Q000502:physiology; D015345:Oligonucleotide Probes; D016376:Oligonucleotides, Antisense; D011498:Protein Precursors / Q000096:biosynthesis; D051381:Rats; D011918:Rats, Inbred SHR; D011919:Rats, Inbred Strains; D011921:Rats, Inbred WKY; D017476:Receptors, Neuropeptide Y / Q000096:biosynthesis*; D012016:Reference Values; D017552:Solitary Nucleus / Q000378:metabolism
chemical_list: D007457:Iodine Radioisotopes; D009478:Neuropeptide Y; D015345:Oligonucleotide Probes; D016376:Oligonucleotides, Antisense; D011498:Protein Precursors; D017476:Receptors, Neuropeptide Y; C042084:preproneuropeptide Y