title: Isolation and characterization of mumps virus strains in a mumps outbreak with a high incidence of aseptic meningitis.
abstract: In 1993, mumps with a high incidence of aseptic meningitis became prevalent in Akita prefecture, Japan. Three mumps virus isolates obtained from the nonvaccine-associated cases lacked the BamHI restriction cleavage site of the P gene, like the Urabe strain (Yamada, A. et al, Vaccine 8: 553-557). However, four additional nucleotide substitutions were found in the determined region of 157 bp. Fourteen of 19 cases from which mumps virus showing the Urabe-like RFLP profile was detected were complicated with symptomatic meningitis, whereas there were only four cases of meningitis among 23 individuals infected with the wild type showing no Urabe-like RFLP profile (non-"Urabe-like" wild-type). The incidence of meningitis was over 70% among patients infected with the "Urabe-like" wild-type virus. The "Urabe-like" wild-type. disappeared after February 1994 in the epidemic area and was replaced by the non-"Urabe-like" wild-type. Patients infected with the "Urabe-like" wild-type lived in a closed colony, in which there were two instances of transmission between siblings. Thus this outbreak was transient and narrowly localized.
authors.affiliation: Department of Microbiology, Akita Prefectural Institute of Public Health, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Saito
authors.forename: Y
authors.initials: Y
authors.lastname: Takahashi
authors.forename: S
authors.initials: S
authors.lastname: Harata
authors.forename: K
authors.initials: K
authors.lastname: Tanaka
authors.forename: T
authors.initials: T
authors.lastname: Sano
authors.forename: T
authors.initials: T
authors.lastname: Suto
authors.forename: A
authors.initials: A
authors.lastname: Yamada
authors.forename: S
authors.initials: S
authors.lastname: Yamazaki
authors.forename: M
authors.initials: M
authors.lastname: Morita
mesh_terms: D001483:Base Sequence; D004196:Disease Outbreaks; D006801:Humans; D007564:Japan; D008582:Meningitis, Aseptic / Q000453:epidemiology*; D008969:Molecular Sequence Data; D009107:Mumps / Q000453:epidemiology / Q000382:microbiology*; D009108:Mumps Vaccine / Q000276:immunology*; D009109:Mumps virus / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D018807:Polymorphism, Single-Stranded Conformational; D015995:Prevalence; D013997:Time Factors
chemical_list: D009108:Mumps Vaccine