Analysis of a short tandem repeat locus on chromosome 19 (D19S253).
A tetranucleotide tandem repeat locus on chromosome 19 (D19S253) was analysed. PCR products were detected by denaturing polyacrylamide gels with fluorescent-based technology. This study has confirmed a polymorphism with 9 alleles ranging from 209 to 241 bp with a simple repeat structure arranged from 7 to 15 repeats. Family studies confirmed mendelian inheritance of alleles. The efficiency on DNA extracted from bloodstains and cigarette butts has been evaluated. The protocol has shown sensitivity and reproducibility.
De Stefano
F
F
Istituto di Medicina Legale, Università degli Studi di Genova, Italy.
Casarino
L
L
Costa
M G
MG
Bruni
G
G
Mannucci
A
A
Unseld
M
M
Hiesel
R
R
Canale
M
M
D000483:Alleles; D001801:Blood Stains; D002874:Chromosome Mapping; D002888:Chromosomes, Human, Pair 19; D005838:Genotype; D006801:Humans; D018895:Microsatellite Repeats / Q000235:genetics; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*