title: Determination of analytical error function for beta-blockers as a possible weighting method for the estimation of the regression parameters.
abstract: Three analytical methods have been developed and validated for the quantification of beta-blockers (celiprolol, bisoprolol and oxprenolol) using high performance liquid chromatography (HPLC) with UV detection. The methods were determined to be linear, precise and accurate (RSDs were lower than 5%), which allowed the quantitation of beta-blockers assayed at concentrations in the range 25-0.78 micrograms ml-1. After validation of reversed-phase HPLC methods, their analytical error functions were established by a rapid, simple and economical procedure. The discrimination of the best function for each active principle was performed by an appropriate polynomial statistical analysis, yielding SD (microgram ml-1) = 0.0295 + 0.0124C - 3.88 x 10(-4)C2 for celiprolol, 0.0199 + 0.011C - 1.27 x 10(-5)C3 for bisoprolol; and 0.0183 + 0.0089C - 9.68 x 10(-6)C3 for oxprenolol. These analytical error functions are an alternative to the weighting methods used in parameter estimation of beta-blockers.
authors.affiliation: Department of Pharmacy, University of Barcelona, Spain.
authors.forename: P
authors.initials: P
authors.lastname: Modamio
authors.forename: C F
authors.initials: CF
authors.lastname: Lastra
authors.forename: E L
authors.initials: EL
authors.lastname: Mariño
mesh_terms: D000319:Adrenergic beta-Antagonists / Q000032:analysis*; D017298:Bisoprolol / Q000032:analysis*; D017272:Celiprolol / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D010096:Oxprenolol / Q000032:analysis*; D012044:Regression Analysis; D015203:Reproducibility of Results
chemical_list: D000319:Adrenergic beta-Antagonists; D010096:Oxprenolol; D017272:Celiprolol; D017298:Bisoprolol