Increased urinary trypsin-inhibitory activity in mercuric chloride induced nephrotoxicity in Wistar rats.
The relationship between trypsin-inhibitory activity (TIA) and the nephrotoxic effects of mercuric chloride (HgCl2)--as illustrated by proteinuria and by a drop in the glomerular filtration rate (GFR) measured by creatinine clearance test (CCT)--was investigated in Wistar rats. HgCl2, 150 or 250 micrograms/100 g BW per day was injected intraperitoneally three times a week for 2 weeks. Both groups showed a significant degree of proteinuria and urinary TIA. Group B (250 micrograms HgCl2/100 g BW) displayed a greater drop in GFR than group A (150 micrograms HgCl2/100 g BW). The urinary TIA was significantly correlated with proteinuria (group A: r = 0.87, group B: r = 0.84), but it was also significantly inversely correlated with the CCT (A: r = -0.96; B: r = -0.88).


CONCLUSIONS
these results suggest that increased urinary TIA may be involved in and indicative of the pathogenesis of mercuric chloride induced nephrotoxicity.
Smetana
S
S
Nephrology Institute, Wolfson Hospital, Holon, Israel.
Khalef
S
S
Zaidel
L
L
Bar-Khayim
Y
Y
Birk
Y
Y
D000818:Animals; D003404:Creatinine / Q000032:analysis; D005260:Female; D007668:Kidney / Q000473:pathology; D007674:Kidney Diseases / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D008627:Mercuric Chloride / Q000506:poisoning*; D008630:Mercury Poisoning / Q000209:etiology / Q000378:metabolism* / Q000473:pathology; D011507:Proteinuria / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D014361:Trypsin Inhibitors / Q000652:urine*
D014361:Trypsin Inhibitors; D008627:Mercuric Chloride; D003404:Creatinine