[Cosmid libraries containing DNA from human chromosome 13].
We characterized two cosmid libraries constructed from flow-sorted chromosome 13 at the Imperial Cancer Research Fund (ICRF), UK (13,000 clones) and Los Alamos National Laboratory (LANL), USA (17,000 clones). After storage for two years, clones showed high viability (95%) and structural stability. EcoR I and Hind III restriction patterns were studied in more than 500 ICRF and 200 LANL cosmids. The average size of inserts was shown to be 35-37 kb in both the libraries. Most cosmids (83% and 93% of ICRF and LANL libraries, respectively) exceed the lower size limit of DNA fragments that can be packaged and represent a good source for physical mapping of chromosome 13. Total length of inserts is four and five genome equivalents in the ICRF and LANL libraries, respectively. ICRF cosmids showed hybridization to 22 of 24 unique probes tested, which corresponds to a 90% probability of having any DNA fragment represented in the library. More than 1 Mb of chromosome 13 is overlapped by 90 cosmids of 22 groups revealed. A chromosomal region of more than 150 kb, containing the ATP1AL1 gene for alpha-1 peptide of Na+, K(+)-ATPase, is covered by 12 cosmids forming a contig. The results of restriction and hybridization analyses are stored in a CLONE database. These data and all the cosmids described are publicly available.
Kapanadze
B I
BI
Brodianskiĭ
V M
VM
Baranova
A V
AV
Sevat'ianov
S Iu
SIu
Fedorova
N D
ND
Kurskov
M M
MM
Kostina
M A
MA
Mironov
A A
AA
Sineokiĭ
S P
SP
Zakhar'ev
V M
VM
Grafodatskiĭ
A S
AS
Modianov
N N
NN
Iankovskiĭ
N K
NK
D002874:Chromosome Mapping; D002882:Chromosomes, Human, Pair 13; D003001:Cloning, Molecular; D003360:Cosmids / Q000235:genetics*; D004247:DNA / Q000235:genetics*; D015342:DNA Probes; D016208:Databases, Factual; D005434:Flow Cytometry; D015698:Genomic Library; D006801:Humans; D009693:Nucleic Acid Hybridization; D015183:Restriction Mapping
D015342:DNA Probes; D004247:DNA
Issledovanie kosmidnykh bibliotek, soderzhashchikh DNK khromosomy 13 cheloveka.