title: Purification of the bovine xanthine oxidoreductase from milk fat globule membranes and cloning of complementary deoxyribonucleic acid.
abstract: The amino acid sequence of the bovine xanthine oxidoreductase was determined by cloning and sequencing cDNA clones encoding the enzyme. Partial amino acid sequence corresponding to 54% of the total sequence were also determined from purified bovine milk xanthine oxidoreductase, showing identity with the translated cDNA sequence. The cDNA of 4719 nucleotides included a 5' untranslated region of 96 nucleotides, an open reading frame encoding a xanthine oxidoreductase of 1332 amino acid residues, and a 3' untranslated region of 624 nucleotides including two polyadenylation signals and a poly (A) tail of 74 nucleotides. The identity between the amino acid sequence of the bovine xanthine oxidoreductase and xanthine oxidoreductase from mammalian species was 86 to 90%.
authors.affiliation: Protein Chemistry Laboratory, University of Aarhus, Denmark.
authors.forename: L
authors.initials: L
authors.lastname: Berglund
authors.forename: J T
authors.initials: JT
authors.lastname: Rasmussen
authors.forename: M D
authors.initials: MD
authors.lastname: Andersen
authors.forename: M S
authors.initials: MS
authors.lastname: Rasmussen
authors.forename: T E
authors.initials: TE
authors.lastname: Petersen
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics*; D005260:Female; D008321:Mammary Glands, Animal / Q000201:enzymology; D008892:Milk / Q000737:chemistry*; D008969:Molecular Sequence Data; D017421:Sequence Analysis; D017385:Sequence Homology; D014968:Xanthine Dehydrogenase / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification*
chemical_list: D018076:DNA, Complementary; D014968:Xanthine Dehydrogenase