Noxious effects of oxygen reactive species on energy-coupling processes in Ehrlich ascites tumor mitochondria and the protection by pantothenic acid.
Irradiation of Ehrlich ascites tumor cells with ultraviolet light or exposure to the Fenton reaction results in lesions in the mitochondrial energy-coupling system. Formation of the membrane potential and its utilization for ATP synthesis are more affected than the respiratory chain. Preincubation of the cells with pantothenic acid or its derivatives which can serve as precursors of CoA largely protects against the damage of mitochondrial energetics by oxygen reactive species formed by UV light or the Fenton reaction. Incubation of Ehrlich ascites tumor cells with pantothenic acid increases their content of glutathione (most of which is present in the reduced form) by 40%. It is concluded that the protective effect of precursors of CoA against lesions of the mitochondrial energy-coupling system by oxygen reactive species is mainly due to removal of free radicals and peroxides by glutathione peroxidase and phospholipid hydroperoxide glutathione peroxidase.
Slyshenkov
V S
VS
Nencki Institute of Experimental Biology, Warsaw, Poland.
Moiseenok
A G
AG
Wojtczak
L
L
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism*; D002463:Cell Membrane Permeability; D005260:Female; D005609:Free Radicals / Q000494:pharmacology; D005978:Glutathione / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007501:Iron / Q000494:pharmacology; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008315:Malondialdehyde / Q000378:metabolism; D008564:Membrane Potentials / Q000187:drug effects; D051379:Mice; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D010101:Oxygen Consumption / Q000187:drug effects; D010205:Pantothenic Acid / Q000494:pharmacology*; D010539:Permeability; D017382:Reactive Oxygen Species; D014407:Tumor Cells, Cultured; D014466:Ultraviolet Rays
C045076:Fenton's reagent; D005609:Free Radicals; D017382:Reactive Oxygen Species; D010205:Pantothenic Acid; D008315:Malondialdehyde; D006861:Hydrogen Peroxide; D007501:Iron; D007770:L-Lactate Dehydrogenase; D005978:Glutathione