title: Limiting impairment of muscle function following ischemia and reperfusion in rabbits.
abstract: Reperfusion injury is a phenomenon complicating microvascular reconstruction. Extensive investigations of free-radical scavengers exist in the literature. The potential beneficial effects of verapamil, deferoxamine, and dimethylsulfoxide were studied in the rabbit forelimb subjected to 3 hr of tourniquet ischemia, followed by a period of reperfusion. Five minutes prior to tourniquet release, rabbits were given single intravenous infusions of normal saline, verapamil (0.2 mg/kg), deferoxamine (50 mg/kg), or dimethylsulfoxide (100 mg/kg). Following reperfusion, neither light microscopy nor laser Doppler flowmetry revealed significant differences between the sham and treated limbs. Muscle function was evaluated by measuring maximal twitch tensions in the flexor digitorum superficialis. Deterioration of muscle contractile function at 0.5, 3, and 24 hr after reperfusion was significantly improved by treatment with verapamil or deferoxamine. Conversely, dimethylsulfoxide worsened muscle function post ischemia. Dimethylsulfoxide does not appear to be beneficial, while verapamil and deferoxamine may be of benefit in mitigating reperfusion injury in microvascular transplantation.
authors.affiliation: Plastic Surgery Research Laboratories, Duke University Medical Center, Durham, North Carolina 27710, USA.
authors.forename: G
authors.initials: G
authors.lastname: Zavitsanos
authors.forename: L
authors.initials: L
authors.lastname: Huang
authors.forename: W
authors.initials: W
authors.lastname: Panza
authors.forename: D
authors.initials: D
authors.lastname: Serafin
authors.forename: B
authors.initials: B
authors.lastname: Klitzman
mesh_terms: D000818:Animals; D002121:Calcium Channel Blockers / Q000627:therapeutic use*; D003676:Deferoxamine / Q000627:therapeutic use*; D004121:Dimethyl Sulfoxide / Q000627:therapeutic use*; D005552:Forelimb; D016166:Free Radical Scavengers / Q000627:therapeutic use*; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects / Q000502:physiology; D018482:Muscle, Skeletal / Q000098:blood supply / Q000503:physiopathology*; D011817:Rabbits; D015427:Reperfusion Injury / Q000503:physiopathology / Q000517:prevention & control*; D013997:Time Factors; D014700:Verapamil / Q000627:therapeutic use*
chemical_list: D002121:Calcium Channel Blockers; D016166:Free Radical Scavengers; D014700:Verapamil; D003676:Deferoxamine; D004121:Dimethyl Sulfoxide