Hyperimmune antirabies sera titration by standard mouse neutralization and counterimmunoelectrophoresis tests, comparing results of different laboratories.
To determine the rabies antibody level of twenty-four hyperimmune equine sera, Standard Mouse Neutralization (SMN) and Couterimmunoelectrophoresis (CIE) tests were carried out, both at the Instituto Butantan (IB) and Instituto Panamericano de Proteccíon de Alimentos y Zoonosis (INPPAZ). Statistical analysis has shown a correlation (r) of 0.9317 between the SMN and CIE performed at the IB, while at the INPPAZ it scored 0.974. Comparison of CIE data of both laboratories yielded a correlation of 0.845. The CIE technique has shown to be a sensitive and efficient as the SMN in titrating antirabies hyperimmune equine sera. Based on CIE results, a simple, rapid and inexpensive technique, titers of sera antibody can be rellably estimated in SMN test.
Díaz
A M
AM
Instituto Panamericano de Proteccíon de Alimentos y Zoonosis (INPPAZ), Buenos Aires, Argentina.
Valentini
E J
EJ
Albas
A
A
Fuches
R M
RM
Gallina
N M
NM
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D003378:Counterimmunoelectrophoresis; D006736:Horses; D007106:Immune Sera / Q000032:analysis*; D051379:Mice; D009500:Neutralization Tests; D011819:Rabies Vaccines / Q000276:immunology*; D011820:Rabies virus / Q000276:immunology*
D000914:Antibodies, Viral; D007106:Immune Sera; D011819:Rabies Vaccines