[Glycyrrhitinic acid reference standard (Control 941) of the National Institute of Health Sciences].
The raw material of glycyrrhitinic acid was examined for preparation of the "Glycyrrhitinic Acid Reference Standard". The candidate material was evaluated physico-chemically by a collaborative study in which five laboratories participated. Analytical data obtained were as follows: UV spectrum, lambda max = 251 nm and specific absorbance E1%1cm (EtOH) at the lambda max = 145; IR spectrum, specific absorption numbers were at 1719, 1654, 1216, and 1170 cm-1; thin-layer chromatography, some laboratories detected a trace amount of one spot and others not detected; high-performance liquid chromatography, 2-6 impurities were detected and the amount of any impurities were estimated to be less than 0.2% and the total amount less than 0.5%. Based on the above results, the candidate material was authorized as the Glycyrrhitinic Acid Reference Standard of National Institute of Health Sciences.
Okada
S
S
Kitajima
A
A
Tanimoto
T
T
Suzuki
H
H
Satake
M
M
D002851:Chromatography, High Pressure Liquid; D006034:Glycyrrhetinic Acid / Q000032:analysis / Q000592:standards*; D006077:Government Agencies; D007564:Japan; D014867:Water / Q000737:chemistry
D014867:Water; D006034:Glycyrrhetinic Acid