title: Indirect stimulatory effects of murine interleukin-12 on in vitro production of nitric oxide by mouse peritoneal cells.
abstract: The effect of murine interleukin-12 (IL-12) on L-arginine-dependent biosynthesis of nitric oxide (NO) by mouse peritoneal cells was evaluated. Interleukin-12 was found to trigger considerably enhanced production of NO in a dose-dependent manner. Antibody neutralization studies indicated that the effect of IL-12 was mediated by IFN-gamma without apparent participation of TNF-alpha. Synergistic effects of IL-12 plus lipopolysaccharide (LPS) were also observed. Our data thus provide evidence that IL-12 is a powerful but indirect modulator of NO formation. These findings may contribute to the better understanding of various biologic effects of IL-12.
authors.affiliation: Institute of Pharmacology, Academy of Sciences of Czech Republic, Prague, Czech Republic.
authors.forename: Z
authors.initials: Z
authors.lastname: Zídek
authors.forename: E
authors.initials: E
authors.lastname: Lotzová
authors.forename: D
authors.initials: D
authors.lastname: Franková
authors.forename: C A
authors.initials: CA
authors.lastname: Savary
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004357:Drug Synergism; D005069:Evaluation Studies as Topic; D005260:Female; D018664:Interleukin-12 / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D009569:Nitric Oxide / Q000096:biosynthesis*; D013268:Stimulation, Chemical
chemical_list: D008070:Lipopolysaccharides; D018664:Interleukin-12; D009569:Nitric Oxide