Random amplified polymorphic DNA typing versus pulsed-field gel electrophoresis for epidemiological typing of vancomycin-resistant enterococci.
Sixty vancomycin-resistant vanA mutant Enterococcus faecium (VRE) isolates, collected during a 40-month period from 48 patients hospitalized in a French Cancer Referral Center, were typed by using random amplified polymorphic DNA (RAPD), and the results were compared with those previously obtained by typing with SmaI pulsed-field gel electrophoresis (PFGE), which is currently recognized as the "gold standard." The discriminating power of RAPD typing, with seven primers and 11 combinations of primers, was tested on 18 strains, and only the most discriminating combination was further tested on the whole collection. We compared the epidemiological usefulness of RAPD typing of 60 clinical VRE isolates with that of SmaI PFGE typing. With primers AP4 and ERIC1R, RAPD generated 30 patterns versus the 36 patterns generated by SmaI PFGE. However, this did not hamper the epidemiologically correct clustering of 15 related strains and the detection of multiple colonization in nine patients. We conclude that this simple RAPD technique is well suited to the epidemiological typing of VRE and the monitoring of its nosocomial spread.
Barbier
N
N
Laboratoire de Bactériologie, CHU de Rouen, Hôpital Charles Nicolle, Rouen, France.
Saulnier
P
P
Chachaty
E
E
Dumontier
S
S
Andremont
A
A
D015373:Bacterial Typing Techniques; D001483:Base Sequence; D003428:Cross Infection / Q000453:epidemiology / Q000382:microbiology; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics*; D004196:Disease Outbreaks; D004352:Drug Resistance, Microbial / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D016983:Enterococcus / Q000145:classification* / Q000187:drug effects / Q000235:genetics*; D005069:Evaluation Studies as Topic; D005602:France / Q000453:epidemiology; D016908:Gram-Positive Bacterial Infections / Q000453:epidemiology / Q000382:microbiology; D006801:Humans; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D019105:Random Amplified Polymorphic DNA Technique; D014640:Vancomycin / Q000494:pharmacology
D017931:DNA Primers; D004269:DNA, Bacterial; D014640:Vancomycin