[Effect of lysostaphin on phagocyte function in burn mice].
The ability of lysostaphin to prevent immunosuppression of phagocyte was assessed in burn mice. A full thickness burn covering 20% of TBSA was created on back of mouse, Lysostaphin was given intraperitoneally (400 micrograms/kg/day) for 3 days in treatment group. The results showed that lysostaphin treatment could restore the depression of phagocytosis of chick RBC by peritoneal macrophages. In the group treated by lysostaphin, peritoneal macrophage chemiluminescence expressed much greater peak value than that in both burn and normal groups. The a-value, which is a criterion of carbon clearance, was 1.63 x 10(-2) in lysostaphin treated mice, while that in burn and normal mice were 8.13 x 10(-4) and 2.76 x 10(-3), respectively. Results of the study indicated that lysostaphin was effective in improving phagocytosis by phagocytes.
Huan
J
J
Burn Institute, Changhai Hospital.
Han
Y
Y
Chen
Y
Y
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D002056:Burns / Q000276:immunology*; D005260:Female; D008248:Lysostaphin / Q000494:pharmacology*; D017737:Macrophages, Peritoneal / Q000276:immunology*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D010587:Phagocytosis / Q000187:drug effects*
D000276:Adjuvants, Immunologic; D008248:Lysostaphin