Apolipoprotein H: a two-step isolation method.
A new method for the purification of apolipoprotein H by affinity chromatography followed by continuous-elution electrophoresis is described. It is both simpler and less complicated than the chromatographic and electrophoretic methods usually used. In addition, apolipoprotein H is isolated in a pure, structurally uncleaved form. This is of importance, as impairment has been detected in commercial preparations. The separation and purification of apolipoprotein H is a necessary prelude to its quantitative determination and phenotyping, and hence the clarification of its physiopathological mechanisms in lipid metabolism.
Gambino
R
R
Istituto di Medicina Interna, Universitá di Torino, Italy.
Ruiu
G
G
Cassader
M
M
Pagano
G
G
D000595:Amino Acid Sequence; D001053:Apolipoproteins / Q000097:blood / Q000235:genetics / Q000302:isolation & purification*; D002846:Chromatography, Affinity / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D006023:Glycoproteins / Q000097:blood / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D050356:Lipid Metabolism; D008969:Molecular Sequence Data; D053482:beta 2-Glycoprotein I
D001053:Apolipoproteins; D006023:Glycoproteins; D053482:beta 2-Glycoprotein I