P-selectin and von Willebrand factor in bovine mesenteric lymphatics: an immunofluorescent study.
P-selectin (PADGEM, GMP-140, CD62) is an integral membrane protein specific to alpha granules of platelets and Weibel-Palade bodies of blood vascular endothelial cells. The presence in lymphatic endothelial cells of numerous Weibel-Palade bodies and their positivity to immunocytochemical reaction for von Willebrand factor have previously been characterized and described. Because von Willebrand factor and P-selectin codistribute in Weibel-Palade bodies of blood vascular endothelial cells we investigated the presence of both P-selectin and von Willebrand factor in lymphatic endothelium. Lymphatic vessels expressed positive reaction to immunocytochemical assay thereby demonstrating the presence of P-selectin in the endothelium. Distribution and intensity of the reaction were similar to those observed in bovine blood vascular endothelium.
Di Nucci
A
A
Istituto di Farmacologia Medica, Università degli Studi di Pavia, I.R.C.C.S. Policlinico S. Matteo, Italy.
Marchetti
C
C
Serafini
S
S
Piovella
F
F
D000818:Animals; D002417:Cattle; D004729:Endothelium, Lymphatic / Q000737:chemistry* / Q000648:ultrastructure; D004730:Endothelium, Vascular / Q000737:chemistry; D005455:Fluorescent Antibody Technique; D008854:Microscopy, Electron; D019007:P-Selectin / Q000032:analysis*; D014841:von Willebrand Factor / Q000032:analysis*
D019007:P-Selectin; D014841:von Willebrand Factor