title: Molecular characterization of an adenylate cyclase gene of the cyanobacterium Spirulina platensis.
abstract: A cyaA gene, encoding an adenylate cyclase, was isolated from a filamentous cyanobacterium, Spirulina platensis, by functional complementation of a cya mutant of Escherichia coli, defective in adenylate cyclase activity. The predicted gene product of cyaA contains a signal peptide-like domain, a putative sensor domain similar to the gene product of vsrA of Pseudomonas solanacearum, a putative membrane-spanning domain and an adenylate cyclase-like catalytic domain. Two other positive clones that complemented the E. coli mutant were isolated from the same cyanobacterium, suggesting that several cya genes are functioning in S. platensis.
authors.affiliation: Department of Biology, Graduate School of Arts and Sciences, University of Tokyo, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Yashiro
authors.forename: T
authors.initials: T
authors.lastname: Sakamoto
authors.forename: M
authors.initials: M
authors.lastname: Ohmori
mesh_terms: D037361:Adenylate Cyclase Toxin; D000262:Adenylyl Cyclases / Q000235:genetics*; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D000458:Cyanobacteria / Q000201:enzymology / Q000235:genetics*; D000242:Cyclic AMP / Q000378:metabolism; D004269:DNA, Bacterial; D006801:Humans; D008969:Molecular Sequence Data; D011498:Protein Precursors / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction
chemical_list: D037361:Adenylate Cyclase Toxin; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D011498:Protein Precursors; D000242:Cyclic AMP; D000262:Adenylyl Cyclases