Differentiating agents do not induce a true hairy cell phenotype in B-CLL cells in vitro.
B-Chronic lymphocytic leukemia (B-CLL) and hairy cell leukemia (HCL) are both differentiated B-cell lymphoproliferative disorders. Prior studies have suggested that phorbol esters and the macrocyclic lactone Bryostatin-1, which are both protein kinase-C activators, can induce the differentiation of B-CLL cells into HCL cells in vitro, as evidenced by morphology, phenotype and TRAP activity. The differentiating effect of all-trans retinoic acid on B-CLL cells has been less extensively studied. We studied the effects of incubating adherence purified B-CLL cells with phorbol myristic acetate (PMA), all-trans retinoic acid (ATRA), and Bryostatin-1. None of these agents induced a true HCL phenotype (CD5-, CD11c/CD25 coexpression) under the conditions studied.
Gartenhaus
R
R
Department of Medicine, Long Island Jewish Medical Center, New Hyde Park, New York, USA.
Hoffman
M
M
Fuchs
A
A
Billet
H
H
Wang
P
P
Gentile
P
P
Rai
K
K
D000135:Acid Phosphatase / Q000032:analysis; D015703:Antigens, CD / Q000032:analysis; D000951:Antigens, Neoplasm / Q000032:analysis; D001402:B-Lymphocytes / Q000187:drug effects* / Q000473:pathology; D014408:Biomarkers, Tumor / Q000032:analysis; D054713:Bryostatins; D002454:Cell Differentiation / Q000187:drug effects; D004789:Enzyme Activation / Q000187:drug effects; D006801:Humans; D016130:Immunophenotyping; D007527:Isoenzymes / Q000032:analysis; D007783:Lactones / Q000494:pharmacology*; D007943:Leukemia, Hairy Cell / Q000473:pathology*; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000473:pathology*; D018942:Macrolides; D014411:Neoplastic Stem Cells / Q000187:drug effects* / Q000473:pathology; D011493:Protein Kinase C / Q000378:metabolism; D000071681:Tartrate-Resistant Acid Phosphatase; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D014212:Tretinoin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D015703:Antigens, CD; D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D054713:Bryostatins; D007527:Isoenzymes; D007783:Lactones; D018942:Macrolides; C046785:bryostatin 1; D014212:Tretinoin; D011493:Protein Kinase C; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase; D013755:Tetradecanoylphorbol Acetate