Survival and maturation of heterotopic fetal brain stem xenografts after treatment with 2-chlorodeoxyadenosine and cyclosporine A.
Brain stem halves from fetal rabbits were transplanted to the caudate nucleus area of adult rats. The animals were treated postoperatively with cyclosporine A (CsA) and 2-chlorodeoxyadenosine (CdA) for three days, and with CdA alone for the next 13 days. The treatment started at the day of implantation, and in some animals it was repeated starting at day 36 after grafting (at the time when signs of a light inflammatory reaction appeared in some grafts). Grafts survived and matured histologically, and no signs of acute rejection were observed up to the 90th day. In some grafts we recorded phasic neuronal activities similar to the respiratory-related neural activities characteristic for the adult brain stem. Immunosuppressive with CdA and CsA deserves further evaluation in fetal brain grafting.
Ryba
M
M
Department of Neurophysiology, Medical Research Centre of the Polish Academy of Sciences, Warsaw, Poland.
Grieb
P
P
Janczewski
W A
WA
Sawicki
J
J
Jagielski
J
J
Andrychowski
J
J
Mantuzyk
J
J
Janisz
M
M
D000818:Animals; D001933:Brain Stem / Q000201:enzymology / Q000254:growth & development / Q000637:transplantation*; D016380:Brain Tissue Transplantation / Q000502:physiology*; D017338:Cladribine / Q000494:pharmacology; D016572:Cyclosporine / Q000494:pharmacology; D005260:Female; D016332:Fetal Tissue Transplantation / Q000502:physiology*; D006085:Graft Survival / Q000187:drug effects*; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D004676:Myelin Basic Protein / Q000096:biosynthesis; D010751:Phosphopyruvate Hydratase / Q000096:biosynthesis; D011247:Pregnancy; D011817:Rabbits; D051381:Rats; D017208:Rats, Wistar; D014183:Transplantation, Heterologous / Q000502:physiology*
D007166:Immunosuppressive Agents; D004676:Myelin Basic Protein; D017338:Cladribine; D016572:Cyclosporine; D010751:Phosphopyruvate Hydratase