Isolation and characterization of mumps virus strains in a mumps outbreak with a high incidence of aseptic meningitis.
In 1993, mumps with a high incidence of aseptic meningitis became prevalent in Akita prefecture, Japan. Three mumps virus isolates obtained from the nonvaccine-associated cases lacked the BamHI restriction cleavage site of the P gene, like the Urabe strain (Yamada, A. et al, Vaccine 8: 553-557). However, four additional nucleotide substitutions were found in the determined region of 157 bp. Fourteen of 19 cases from which mumps virus showing the Urabe-like RFLP profile was detected were complicated with symptomatic meningitis, whereas there were only four cases of meningitis among 23 individuals infected with the wild type showing no Urabe-like RFLP profile (non-"Urabe-like" wild-type). The incidence of meningitis was over 70% among patients infected with the "Urabe-like" wild-type virus. The "Urabe-like" wild-type. disappeared after February 1994 in the epidemic area and was replaced by the non-"Urabe-like" wild-type. Patients infected with the "Urabe-like" wild-type lived in a closed colony, in which there were two instances of transmission between siblings. Thus this outbreak was transient and narrowly localized.
Saito
H
H
Department of Microbiology, Akita Prefectural Institute of Public Health, Japan.
Takahashi
Y
Y
Harata
S
S
Tanaka
K
K
Sano
T
T
Suto
T
T
Yamada
A
A
Yamazaki
S
S
Morita
M
M
D001483:Base Sequence; D004196:Disease Outbreaks; D006801:Humans; D007564:Japan; D008582:Meningitis, Aseptic / Q000453:epidemiology*; D008969:Molecular Sequence Data; D009107:Mumps / Q000453:epidemiology / Q000382:microbiology*; D009108:Mumps Vaccine / Q000276:immunology*; D009109:Mumps virus / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D018807:Polymorphism, Single-Stranded Conformational; D015995:Prevalence; D013997:Time Factors
D009108:Mumps Vaccine