title: Fructose 2,6-bisphosphate induces irreversible transitions in cell-free extracts of rat liver.
abstract: The effect of fructose 2,6-bisphosphate on the dynamics of the 6-phosphofructo-1-kinase/fructose-1,6-bisphosphatase cycle is investigated in a cell-free extract of rat liver under steady-state conditions. Bistability emerges on the basis of the reciprocal allosteric modulation of 6-phosphofructo-1-kinase and fructose-1,6-bisphosphatase. Under conditions of bistability fructose 2,6-bisphosphate may cause transitions between alternative steady states. However, in contrast to what is frequently observed in bistable systems, within a broad range of experimental conditions these transitions proceed irreversibly from states with high ATP to states characterized by low ATP.
authors.affiliation: Institute of Biochemistry, University of Leipzig, School of Medicine, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Frenzel
authors.forename: W
authors.initials: W
authors.lastname: Schellenberger
authors.forename: K
authors.initials: K
authors.lastname: Eschrich
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002474:Cell-Free System; D004789:Enzyme Activation / Q000187:drug effects; D004795:Enzyme Stability / Q000187:drug effects; D006597:Fructose-Bisphosphatase / Q000378:metabolism; D005635:Fructosediphosphates / Q000378:metabolism / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D008099:Liver / Q000187:drug effects* / Q000378:metabolism*; D008297:Male; D010732:Phosphofructokinase-1 / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
chemical_list: D005635:Fructosediphosphates; C027652:fructose 2,6-diphosphate; D000255:Adenosine Triphosphate; D010732:Phosphofructokinase-1; D006597:Fructose-Bisphosphatase