title: Protein kinase C inhibitor H-7 blocks effects of tumor necrosis factor on bone cells.
abstract: OBJECTIVE
  To study the effects of tumor necrosis factor (TNF) on the neonatal mouse osteoblast-enriched calvarial cells and effects of protein kinase C (PK C) inhibitor, 1-(5-isoquinolinesulfonyl)-2 -methylpiperazine (H-7) on the TNF actions.
  
  
  METHODS
  [3H]TdR uptake by the osteoblasts was used to measure cell proliferation. Cellular alkaline phosphatase (AIP) and tartrate resistant acid phosphatase (trAcP) activities were determined spectrophotometrically.
  
  
  RESULTS
  TNF (1-100 kU . L-1) inhibited both proliferation and expression of AIP activity, but stimulated trAcP activity. These TNF-induced actions were blocked by simultaneous addition of H-7 (5-20 mumol . L-1).
  
  
  CONCLUSIONS
  TNF has potent effects on the osteoblasts, and the blockade of TNF actions by H-7 suggests that TNF exert its effects through PK C.
authors.affiliation: Research Laboratory of Natural and Synthetic Drugs, School of Pharmacy, Second Military Medical University, Shanghai, China.
authors.forename: T M
authors.initials: TM
authors.lastname: Wu
authors.forename: J P
authors.initials: JP
authors.lastname: Zhang
authors.forename: Z L
authors.initials: ZL
authors.lastname: Hu
authors.forename: D H
authors.initials: DH
authors.lastname: Qian
mesh_terms: D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine / Q000494:pharmacology*; D000135:Acid Phosphatase / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D051379:Mice; D008813:Mice, Inbred ICR; D010006:Osteoblasts / Q000166:cytology* / Q000201:enzymology; D011493:Protein Kinase C / Q000037:antagonists & inhibitors*; D012886:Skull / Q000166:cytology; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors*
chemical_list: D004791:Enzyme Inhibitors; D014409:Tumor Necrosis Factor-alpha; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D011493:Protein Kinase C; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase