title: Abortive mitoses and nuclear DNA fragmentation in CD30+ large cells of Hodgkin's disease.
abstract: This study was undertaken to better comprehend the reasons for the scarcity of Hodgkin and Reed-Sternberg (H-RS) cells in Hodgkin's disease (HD) despite their expression of "proliferation-associated antigens". To this end, we assessed the relative frequency of mitotic phases and nuclear damage (detected by in situ end-labeling of DNA strand breaks) in CD30+ large cells of nodular sclerosis and mixed cellularity HD. Our results show that a) most CD30+ cells in HD exhibit abortive mitoses, with a highly significant arrest at the metaphase-ana/telophase transition, and b) many of these elements, i.e. mainly H-RS cells, show fragmentation of nuclear DNA, suggesting imminent or actual death. Percentages of CD30+ cells that entered mitosis and those with DNA strand breaks were of a similar order of magnitude and correlated significantly in a linear fashion. These findings are consistent with the concept that cell deletion is the major cause of the paucity of H-RS cells in HD.
authors.affiliation: Institute of Pathologic Anatomy and Histology, University of Siena, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Leoncini
authors.forename: D
authors.initials: D
authors.lastname: Spina
authors.forename: P
authors.initials: P
authors.lastname: Close
authors.forename: T
authors.initials: T
authors.lastname: Megha
authors.forename: L
authors.initials: L
authors.lastname: Pacenti
authors.forename: P
authors.initials: P
authors.lastname: Tosi
authors.forename: S
authors.initials: S
authors.lastname: Pileri
authors.forename: A
authors.initials: A
authors.lastname: De Vivo
authors.forename: R
authors.initials: R
authors.lastname: Kraft
authors.forename: J A
authors.initials: JA
authors.lastname: Laissue
authors.forename: H
authors.initials: H
authors.lastname: Cottier
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000951:Antigens, Neoplasm / Q000032:analysis*; D017209:Apoptosis; D002455:Cell Division; D053938:DNA Fragmentation; D004273:DNA, Neoplasm / Q000032:analysis*; D005260:Female; D006689:Hodgkin Disease / Q000235:genetics / Q000473:pathology*; D006801:Humans; D017730:Ki-1 Antigen / Q000032:analysis*; D008228:Lymphoma, Non-Hodgkin / Q000473:pathology; D008297:Male; D008875:Middle Aged; D008938:Mitosis; D016539:Reed-Sternberg Cells / Q000737:chemistry / Q000473:pathology*
chemical_list: D000951:Antigens, Neoplasm; D004273:DNA, Neoplasm; D017730:Ki-1 Antigen