title: Antibodies to bovine serum albumin in Brazilian children and young adults with IDDM.
abstract: OBJECTIVE
  To evaluate the prevalence of IgG antibodies to bovine serum albumin (BSA) in a cohort of Brazilian children and young adults with IDDM.
  
  
  METHODS
  Sera from 81 subjects with < 1 year of IDDM (group 1), III subjects with > 1 year of IDDM (group 2), and 207 normoglycemic subjects were tested using an immunofluorimetric assay. A receiver-operating-characteristic curve was used to establish the threshold of anti-BSA antibody titers defining the positivity of the assay.
  
  
  RESULTS
  The distribution of the fluorimetric index (FI) of anti-BSA antibodies did not have a gaussian profile. Rank sum of FI was significantly higher in patients than in control subjects (P < 0.0001). Average logFI values of both IDDM groups were significantly higher than that of the control group (P < 0.005 for both groups). There was a trend toward higher FI levels in group 1 than in group 2 (P = 0.06). A FI cutoff of 0.7 optimized the ratio of true-positive to false-positive of the assay, with the best equilibrium between sensitivity and specificity. The prevalence of anti-BSA antibodies was 52% in group 1, 47% in group 2, and 28% in the control group (P = 0.0001). An independent association between anti-BSA antibodies and IDDM, with an odds ratio of 3.03 (P < 0.0001), was observed in a logistic regression analysis. However anti-BSA antibodies explained only 5% of the variability of IDDM versus NIDDM.
  
  
  CONCLUSIONS
  Our results confirm that the prevalence of anti-BSA antibodies is higher in IDDM subjects than in control subjects, even after 1 year of diabetes. However, a large overlap of antibody titers is observed in patients and control subjects, suggesting that anti-BSA antibodies are neither sensitive nor specific markers of IDDM.
authors.affiliation: Division of Endocrinology, Escola Paulista de Medicina, Sao Paulo, Brazil. vpardini@embratel.net.br
authors.forename: V C
authors.initials: VC
authors.lastname: Pardini
authors.forename: J G
authors.initials: JG
authors.lastname: Vieira
authors.forename: W
authors.initials: W
authors.lastname: Miranda
authors.forename: S R
authors.initials: SR
authors.lastname: Ferreira
authors.forename: G
authors.initials: G
authors.lastname: Velho
authors.forename: E M
authors.initials: EM
authors.lastname: Russo
mesh_terms: D000293:Adolescent; D000328:Adult; D000906:Antibodies / Q000097:blood*; D001938:Brazil; D002648:Child; D003922:Diabetes Mellitus, Type 1 / Q000097:blood / Q000276:immunology*; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000276:immunology; D005189:False Positive Reactions; D005260:Female; D006801:Humans; D008297:Male; D012016:Reference Values; D012044:Regression Analysis; D012680:Sensitivity and Specificity; D012710:Serum Albumin, Bovine / Q000276:immunology*; D018709:Statistics, Nonparametric
chemical_list: D000906:Antibodies; D012710:Serum Albumin, Bovine