Proctolinlike immunoreactivity and identified neurosecretory cells as putative substrates for modulation of courtship display behavior in the blue crab, Callinectes sapidus.
Pheromonally stimulated courtship display (CD) behavior in male blue crabs (Callinectes sapidus) is characterized by rhythmic waving of the fifth legs. The waving of the fifth legs is modulated by proctolin in freely moving crabs and in reduced preparations. To begin to identify an anatomical substrate for CD behavior we have localized putative proctolinergic cells and described the morphology of neurosecretory neurons known to oscillate during pheromonal stimulation of reduced preparations. Proctolin-induced CD occurs with developmental and seasonal dependence. Male crabs altered hormonally by eyestalk ligation spontaneously produce CD behavior. We have localized proctolinlike immunoreactivity (PIR) in the central nervous system (CNS) and compared this immunoreactivity across sexes, developmental stage, eyestalk ligation, and seasonal conditions to determine whether or not expression of PIR is correlated with CD behavior. PIR was found in most areas of the CNS. Clusters of PIR-positive cells were found in the sinus gland and eyestalk ganglia, olfactory neuropil with associated cell bodies, and in a large cell cluster in the subesophageal region of the ventral nerve cord. Three pairs of cell bodies in different cell body groups in the brains of adult crabs stained positively for PIR but did not stain in the youngest juvenile animals. Comparison of PIR distribution with toluidine blue studies of the ventral nerve cord indicated a high likelihood that cells in the PIR-positive cluster of the subesophageal ganglia were also members of the cluster of neurons identified as oscillatory neurosecretory neurons.
Wood
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Department of Biology, Georgia State University, Atlanta 30302-4010, USA. dwood@neurbio.manduca.arizona.edu
Nishikawa
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Derby
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CD
D000818:Animals; D003386:Brachyura / Q000502:physiology*; D001921:Brain / Q000166:cytology*; D001923:Brain Chemistry / Q000502:physiology; D003035:Cobalt; D004396:Coloring Agents; D005260:Female; D017952:Ganglia, Invertebrate / Q000166:cytology / Q000378:metabolism; D007150:Immunohistochemistry; D008297:Male; D009412:Nerve Fibers / Q000502:physiology / Q000648:ultrastructure; D009479:Neuropeptides; D009490:Neurosecretory Systems / Q000166:cytology* / Q000378:metabolism*; D018377:Neurotransmitter Agents / Q000378:metabolism*; D009842:Oligopeptides / Q000378:metabolism*; D012621:Seasons; D012727:Sex Characteristics; D012726:Sexual Behavior, Animal / Q000502:physiology*; D014048:Tolonium Chloride
D004396:Coloring Agents; D009479:Neuropeptides; D018377:Neurotransmitter Agents; D009842:Oligopeptides; D014048:Tolonium Chloride; D003035:Cobalt; C011536:proctolin