title: Evaluating efficiency of bone marrow harvest and its manipulation--role of CD 34 positivity.
abstract: We evaluated harvested marrow cells for total nucleated cells (27.49 x 10(9)), absolute 'lymphocyte' count (6.29 x 10(9)) and CD 34 positive cells (3.57 x 10(9)). The same parameters were studied after in vitro manipulation to remove RBCs and plasma. Reinfused WBCs contained 12.87 x 10(9) nucleated cells, 4.25 x 10(9) absolute 'lymphocyutes' and 3.34 x 10(9) CD 34 positive cells. The corresponding figures for loss during in vitro manipulation (tubing, RBCs and plasma) are 14.62 (53.18%), 2.04 (32.43%) and 0.23 x 10(9) (6.44%) cells respectively. Therefore CD 34 positivity may be a better indicator of total yield, loss during manipulation and reinfusion of hemopoietic progenitor cells in bone marrow transplantation.
authors.affiliation: Department of Medical Oncology, Tata Memorial Hospital, Parel, Bombay.
authors.forename: P M
authors.initials: PM
authors.lastname: Parikh
authors.forename: Y
authors.initials: Y
authors.lastname: Badrinath
authors.forename: S
authors.initials: S
authors.lastname: Dhond
authors.forename: S
authors.initials: S
authors.lastname: Kulkarni
authors.forename: A K
authors.initials: AK
authors.lastname: Dhar
authors.forename: N
authors.initials: N
authors.lastname: Raje
authors.forename: T K
authors.initials: TK
authors.lastname: Saikia
authors.forename: S
authors.initials: S
authors.lastname: Bharbhaya
authors.forename: R
authors.initials: R
authors.lastname: Gopal
authors.forename: P A
authors.initials: PA
authors.lastname: Kurkure
mesh_terms: D018952:Antigens, CD34 / Q000032:analysis*; D001854:Bone Marrow Cells; D001856:Bone Marrow Examination; D016465:Bone Marrow Purging; D016026:Bone Marrow Transplantation; D016238:Cytapheresis; D004912:Erythrocytes; D005260:Female; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000276:immunology; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000628:therapy; D007958:Leukocyte Count; D018655:Lymphocyte Count; D010949:Plasma; D010956:Plasmapheresis
chemical_list: D018952:Antigens, CD34