Distribution of gamma delta T cells in the pig foetus.
The distribution of gamma delta T cells during different developmental stages of pig foetuses was studied using monoclonal antibodies directed against a ruminant WC1 antigen, a T-cell receptor (TCR) subset epitope, and pig Null T-cell antigen. Binding was revealed by the avidin/biotin/peroxidase technique on cryostat sections and flow cytometry analysis. The extrathymic origin of gamma delta T cells was confirmed: gamma delta T cells appeared in the liver, spleen and peripheral blood sooner than in the thymus. In the course of the second half of gestation, these T cells were found in the dermis and intestinal mucosal compartments which represented the predominant loci of gamma delta T cells after birth.
Trebichavský
I
I
Institute of Microbiology, Division of Immunology and Gnotobiology, Praha, Czech Republic.
Sinkora
J
J
Reháková
Z
Z
Splíchal
I
I
Whyte
A
A
Binns
R
R
Pospísil
R
R
Tucková
L
L
D000818:Animals; D000911:Antibodies, Monoclonal; D005260:Female; D005333:Fetus / Q000166:cytology* / Q000276:immunology; D005434:Flow Cytometry; D009928:Organ Specificity; D011247:Pregnancy; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000032:analysis / Q000276:immunology*; D013552:Swine; D013601:T-Lymphocytes / Q000166:cytology* / Q000276:immunology
D000911:Antibodies, Monoclonal; D016692:Receptors, Antigen, T-Cell, gamma-delta