HLA-B27 determination by polymerase chain reaction.
A method for determining the presence or absence of HLA-B27 by selective amplification of a region in the third exon of the HLA-B27 gene common to B*2701 to B*2705 inclusive, was evaluated. This polymerase chain reaction/sequence specific primer (PCR-SSP) method gave perfect correlation with serological typing on 40 individuals of previously determined HLA type and on 50 further clinical samples evaluated blind. It was concluded that HLA-B27 determination by PCR-SSP is simple, reliable, cost-effective and convenient for laboratory staff.
Kilpatrick
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D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D003951:Diagnostic Errors; D005819:Genetic Markers; D005838:Genotype; D015796:HLA-B27 Antigen / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D011446:Prospective Studies; D012189:Retrospective Studies
D017931:DNA Primers; D005819:Genetic Markers; D015796:HLA-B27 Antigen