[Expression of HIV-1 epitopes included in particles formed by human hepatitis B virus nucleocapsid protein].
Hybrids of the core protein of hepatitis B virus (HBcAg) have been designed which carry N-terminal insertions of B- and T-cell epitopes of HIV-1 an immunodominant B-epitope from gp41, a T-cell epitope from p34 pol, and a cluster of B- and T-cell epitopes from p17 gag. The hybrids have been synthesized using two expression systems-one based on the thermoinducible PR promoter of bacteriophage lambda and the other one based on phi 10 promoter of bacteriophage T7 with 3-5% and 7-14% yields, respectively. The hybrids have dual HBV and HIV-1 immunospecificity and are assembled into particles similar to those formed by the protein carrier HBcAg. Sandwich ELISA and immune electron microscopy revealed that HIV-1 epitopes are exposed on the surface of the particles.
Isaguliants
M G
MG
Swedish Institute for Infectious Disease Control, Stockholm, Sweden.
Kadoshnikov
Iu P
IuP
Kalinina
T I
TI
Khuliakov
Iu E
IuE
Semiletov
Iu A
IuA
Smirnov
V D
VD
Wahren
B
B
D000595:Amino Acid Sequence; D010582:Bacteriophage lambda / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular; D004274:DNA, Recombinant; D000939:Epitopes / Q000235:genetics*; D015497:HIV-1 / Q000276:immunology*; D006512:Hepatitis B Core Antigens / Q000235:genetics*; D006801:Humans; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000235:genetics* / Q000648:ultrastructure
D004274:DNA, Recombinant; D000939:Epitopes; D006512:Hepatitis B Core Antigens; D011993:Recombinant Fusion Proteins
Ekspressiia épitopov VICh-1 v sostave chastits, obrazuemykh belkom nukleokapsida virusa gepatita B cheloveka.