Abortive mitoses and nuclear DNA fragmentation in CD30+ large cells of Hodgkin's disease.
This study was undertaken to better comprehend the reasons for the scarcity of Hodgkin and Reed-Sternberg (H-RS) cells in Hodgkin's disease (HD) despite their expression of "proliferation-associated antigens". To this end, we assessed the relative frequency of mitotic phases and nuclear damage (detected by in situ end-labeling of DNA strand breaks) in CD30+ large cells of nodular sclerosis and mixed cellularity HD. Our results show that a) most CD30+ cells in HD exhibit abortive mitoses, with a highly significant arrest at the metaphase-ana/telophase transition, and b) many of these elements, i.e. mainly H-RS cells, show fragmentation of nuclear DNA, suggesting imminent or actual death. Percentages of CD30+ cells that entered mitosis and those with DNA strand breaks were of a similar order of magnitude and correlated significantly in a linear fashion. These findings are consistent with the concept that cell deletion is the major cause of the paucity of H-RS cells in HD.
Leoncini
L
L
Institute of Pathologic Anatomy and Histology, University of Siena, Italy.
Spina
D
D
Close
P
P
Megha
T
T
Pacenti
L
L
Tosi
P
P
Pileri
S
S
De Vivo
A
A
Kraft
R
R
Laissue
J A
JA
Cottier
H
H
D000293:Adolescent; D000328:Adult; D000368:Aged; D000951:Antigens, Neoplasm / Q000032:analysis*; D017209:Apoptosis; D002455:Cell Division; D053938:DNA Fragmentation; D004273:DNA, Neoplasm / Q000032:analysis*; D005260:Female; D006689:Hodgkin Disease / Q000235:genetics / Q000473:pathology*; D006801:Humans; D017730:Ki-1 Antigen / Q000032:analysis*; D008228:Lymphoma, Non-Hodgkin / Q000473:pathology; D008297:Male; D008875:Middle Aged; D008938:Mitosis; D016539:Reed-Sternberg Cells / Q000737:chemistry / Q000473:pathology*
D000951:Antigens, Neoplasm; D004273:DNA, Neoplasm; D017730:Ki-1 Antigen