Characterization of the 3' UTR sequence encoded by the AQP-1 gene in human retinal pigment epithelium.
The complete 3' UTR sequence encoded by the human aquaporin-1 gene is reported. The sequence encompassed by two cDNA clones showed, 33 nucleotides of 5' UTR sequence, a coding sequence of 807 nucleotides and 1886 nucleotides corresponding to the complete 3' UTR sequence. High similarity with 3' UTR sequences from rat and mouse counterparts was found. Northern blot analysis of several human tissues revealed a 2.8 kbp transcript. These data confirm the existence of water channels in the human retinal pigment epithelium.
Ruiz
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Department of Neurobiology, School of Medicine, University of California, Los Angeles 90024, USA.
Bok
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D
D000818:Animals; D051398:Aquaporin 1; D020346:Aquaporins; D001483:Base Sequence; D001789:Blood Group Antigens; D015152:Blotting, Northern; D001923:Brain Chemistry; D018076:DNA, Complementary / Q000737:chemistry; D005347:Fibroblasts / Q000737:chemistry; D006801:Humans; D007473:Ion Channels / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D010857:Pigment Epithelium of Eye / Q000737:chemistry*; D051381:Rats; D016415:Sequence Alignment
C496020:AQP1 protein, human; C496021:Aqp1 protein, mouse; C496022:Aqp1 protein, rat; D020346:Aquaporins; D001789:Blood Group Antigens; D018076:DNA, Complementary; D007473:Ion Channels; D051398:Aquaporin 1