title: Effect of trimethylamine-carbomethoxyborane on growth traits and lipid metabolism in lines of mice selected for high and low fat content.
abstract: The purpose of this study was to determine the effects of the amine carboxyborane, trimethylamine-carbomethoxyborane (B), on growth, body composition and lipid metabolism in lines of mice differing in fat content as a result of directional selection: high fat content (HF); low fat content (LF); and random control (RC). Mice were injected i.p. daily with either 20 mg/kg of B dissolved in 1% (w/v) carbomethoxycellulose as the vehicle or just vehicle (C) from 26 to 42 days of age. Growth rate, feed intake, feed efficiency, epididymal fat pad weight/BW and percentage body water were not affected by B treatment, but liver weight/BW was increased (P < 0.001). HF mice had larger epididymal fat pad weight/BW and less percentage body water when compared to LF mice (P < 0.001). Treatment with B lowered (P < 0.01) serum triglyceride and cholesterol levels, and selection for divergence in fat content led to positive divergence (P < 0.01, (HF-LF) > 0) in serum triglyceride and serum cholesterol levels; divergence x treatment interactions (P < 0.01) were due to administration of B eliminating the line differences present when C was administered. Treatment with B elevated (P < 0.001) HDL cholesterol level and lowered (P < 0.001) chylomicron, LDL and VLDL cholesterol levels, while positive divergence was found for all four fractions. Fecal triglyceride and cholesterol levels were increased (P < 0.01) by administering B, but were not affected by selection. Hepatic enzyme activity of cholesterol-7 alpha-hydroxylase was elevated (P < 0.001) by treatment with B while activities of other hepatic enzymes were depressed, e.g., acyl-CoA cholesterol acyltransferase. Line differences in activity of several hepatic enzymes also were found. These results indicate that B acts as a hypolipidemic agent in both high-fat and low-fat mouse genotypes, lowering serum cholesterol and triglyceride levels. However, at the administered dose, B had no affect on growth rate, feed intake, feed efficiency or body fat content.
authors.affiliation: Department of Animal Science, North Carolina State University, Raleigh 27695-7621, USA.
authors.forename: E J
authors.initials: EJ
authors.lastname: Eisen
authors.forename: E E
authors.initials: EE
authors.lastname: Jones
authors.forename: K G
authors.initials: KG
authors.lastname: Rajendran
authors.forename: I H
authors.initials: IH
authors.lastname: Hall
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn; D001823:Body Composition / Q000187:drug effects*; D001880:Boranes / Q000494:pharmacology*; D002784:Cholesterol / Q000378:metabolism; D002790:Cholesterol 7-alpha-Hydroxylase / Q000378:metabolism; D006128:Growth / Q000187:drug effects* / Q000502:physiology; D050356:Lipid Metabolism; D008099:Liver / Q000378:metabolism; D008297:Male; D008744:Methylamines / Q000494:pharmacology*; D051379:Mice; D008806:Mice, Inbred AKR; D008807:Mice, Inbred BALB C; D008811:Mice, Inbred DBA; D009765:Obesity / Q000378:metabolism*; D011897:Random Allocation; D002785:Sterol O-Acyltransferase / Q000378:metabolism; D014280:Triglycerides / Q000378:metabolism
chemical_list: D001880:Boranes; D008744:Methylamines; D014280:Triglycerides; D002784:Cholesterol; D002790:Cholesterol 7-alpha-Hydroxylase; D002785:Sterol O-Acyltransferase; C023336:trimethylamine