title: Characterization of an Arabidopsis thaliana cDNA encoding an S-adenosylmethionine-sensitive threonine synthase. Threonine synthase from higher plants.
abstract: An Arabidopsis thaliana cDNA encoding an S-adenosylmethionine-sensitive threonine synthase (EC 4.2.99.2) has been isolated by functional complementation of an Escherichia coli mutant devoid of threonine synthase activity. Threonine synthase from A. thaliana was shown to be synthesized with a transit peptide. The recombinant protein is activated by S-adenosylmethionine in the same range as the plant threonine synthase and evidence is presented for an involvement of the N-terminal part of the mature enzyme in the sensitivity to S-adenosylmethionine.
authors.affiliation: Laboratoire Mixte, Centre National de la Recherche Scientifique/Rhône-Poulenc UMR-41, Lyon, France.
authors.forename: G
authors.initials: G
authors.lastname: Curien
authors.forename: R
authors.initials: R
authors.lastname: Dumas
authors.forename: S
authors.initials: S
authors.lastname: Ravanel
authors.forename: R
authors.initials: R
authors.lastname: Douce
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics; D001483:Base Sequence; D019757:Carbon-Oxygen Lyases; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003001:Cloning, Molecular; D017124:Conserved Sequence; D017931:DNA Primers; D018076:DNA, Complementary; D004926:Escherichia coli; D007700:Kinetics; D008190:Lyases / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism*; D008239:Lysine; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism; D012436:S-Adenosylmethionine / Q000494:pharmacology*; D017386:Sequence Homology, Amino Acid
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D011994:Recombinant Proteins; D012436:S-Adenosylmethionine; D008190:Lyases; D019757:Carbon-Oxygen Lyases; C023160:threonine synthase; D008239:Lysine