title: Histochemical study of muscle fiber types in Synbranchus marmoratus bloch, 1795.
abstract: The myotomal muscle of Synbranchus marmoratus was investigated using histochemical and immunohistochemical reactions. This musculature is composed of a superficial red compartment, uniformly distributed around the trunk circumferentially and also in the lateral line. The red compartment fibers are small in diameter and have an oxidative metabolism, a high rate of glycogen and a negative reaction to alkaline and acid myofibrillar ATPase (mATPase). The white muscle forms the bulk of the muscle mass. Its fibers are large in diameter and have a glycolytic metabolism, a negative reaction to glycogen, a strong reaction to alkaline mATPase and a negative reaction to acid mATPase. Between these two compartments there is an intermediate layer of fibers presenting a mosaic metabolism pattern with a high rate of glycogen. These fibers stained moderately for alkaline and acid m-ATPase. Several clusters of red muscles were observed inside the white muscle. Each cluster is composed of three fiber types, with a predominance of red and intermediate fibers. Reactivity to anti-MHC BA-D5 was positive only in the intermediate fibers. Reactivity to anti-MHC SC-71 was negative in all fiber types.
authors.affiliation: Departamento de Morfologia, Instituto de Biociências, UNESP, Botucatu, Brasil.
authors.forename: M
authors.initials: M
authors.lastname: Dal Pai-Silva
authors.forename: V
authors.initials: V
authors.lastname: Dal Pai
authors.forename: D L
authors.initials: DL
authors.lastname: da Mota
authors.forename: A
authors.initials: A
authors.lastname: de Castro Rodrigues
mesh_terms: D000251:Adenosine Triphosphatases / Q000032:analysis; D000818:Animals; D001835:Body Weight; D008058:Dihydrolipoamide Dehydrogenase / Q000032:analysis; D005399:Fishes / Q000033:anatomy & histology*; D006003:Glycogen / Q000032:analysis; D006019:Glycolysis; D006651:Histocytochemistry / Q000379:methods; D018656:Muscle Fibers, Fast-Twitch / Q000166:cytology / Q000378:metabolism; D018485:Muscle Fibers, Skeletal / Q000166:cytology* / Q000378:metabolism; D018482:Muscle, Skeletal / Q000166:cytology* / Q000378:metabolism; D009210:Myofibrils / Q000201:enzymology / Q000648:ultrastructure
chemical_list: D006003:Glycogen; D008058:Dihydrolipoamide Dehydrogenase; D000251:Adenosine Triphosphatases