The human anion transport protein, band 3, contains a CD36-like binding domain for Plasmodium falciparum-infected erythrocytes.
Epitope mapping of a murine monoclonal antibody (mAb), 5H12, prepared against live Plasmodium falciparum-infected red blood cells indicated that the epitope consisted of amino acid residues 474-487 of the human anion transport protein, band 3. mAb 5H12 enhanced cytoadherence, but inhibited the CD36-like mediated rosetting. A synthetic peptide based on the sequence of the epitope (FSFCETNGLE) blocked both rosetting and cytoadherence, suggesting that this amino acid sequence may form the CD36-like receptor. The CD36-like region of band 3 was antigenically distinct from platelet or endothelial CD36.
Crandall
I
I
Department of Biology, University of California, Riverside 92521, USA.
Sherman
I W
IW
D000595:Amino Acid Sequence; D000818:Animals; D001457:Anion Exchange Protein 1, Erythrocyte / Q000737:chemistry / Q000502:physiology*; D000911:Antibodies, Monoclonal; D001665:Binding Sites; D001792:Blood Platelets / Q000276:immunology; D018955:CD36 Antigens / Q000737:chemistry / Q000502:physiology*; D002448:Cell Adhesion; D004730:Endothelium, Vascular / Q000276:immunology; D000939:Epitopes / Q000032:analysis; D004910:Erythrocyte Membrane / Q000469:parasitology*; D006790:Host-Parasite Interactions; D006801:Humans; D007140:Immunoglobulin Fab Fragments; D051379:Mice / Q000276:immunology; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000494:pharmacology; D010963:Plasmodium falciparum / Q000502:physiology*; D012397:Rosette Formation
D001457:Anion Exchange Protein 1, Erythrocyte; D000911:Antibodies, Monoclonal; D018955:CD36 Antigens; D000939:Epitopes; D007140:Immunoglobulin Fab Fragments; D010446:Peptide Fragments