title: Colonization of streptomycin-treated mice by Aeromonas species.
abstract: Streptomycin-treated adult mice were investigated as a possible model for studying the enteropathogenicity of Aeromonas species. C57BL mice pre-treated with streptomycin (5.0 g/L drinking water, 48 hours) received a single intragastric dose (10(10) bacteria /10.5 mL) of one of six well-characterized, toxin-producing, human diarrhoeal isolates of A. veronii biovar sobria (n = 3) or A. hydrophila (n = 3). Their faeces were examined for Aeromonas for 10 days post-challenge. All strains colonized the antibiotic-treated mice. Colonization did not occur in mice which did not receive streptomycin. Strains of A. hydrophila were recovered in greater numbers than strains of A. veronii biovar sobria, and colonized ( > or = 10(3) cfu/g of faeces) a greater proportion of mice at day 10. Strains of the latter species, however, were more adherent in cell line assays used as models of intestinal adhesion. A. hydrophila strains localized in the large intestine and appeared not to be cell associated. This study, therefore, points to species-related differences in intestinal colonization mechanisms. The streptomycin-treated adult mouse model may prove useful for further investigation of some of these mechanisms. Diarrhoeal symptoms were, however, not produced in this model.
authors.affiliation: Department of Pathology, University of Tasmania, Australia.
authors.forename: K
authors.initials: K
authors.lastname: Sanderson
authors.forename: F M
authors.initials: FM
authors.lastname: Ghazali
authors.forename: S M
authors.initials: SM
authors.lastname: Kirov
mesh_terms: D000333:Aeromonas / Q000254:growth & development / Q000472:pathogenicity*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000494:pharmacology*; D015169:Colony Count, Microbial; D004195:Disease Models, Animal; D016905:Gram-Negative Bacterial Infections; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D013307:Streptomycin / Q000008:administration & dosage / Q000494:pharmacology*; D014774:Virulence
chemical_list: D000900:Anti-Bacterial Agents; D013307:Streptomycin