title: [Cloning and sequencing of the gene PE40, coding for a portion of Pseudomonas exotoxin A].
abstract: Gene PE40 coding for a mutant form of Pseudomonas Exotoxin A was devoid of its native cell binding domain, and was amplified from the total DNA of P. aeruginosa PA103 by PCR. After being sequenced, it was cloned into expressional vector pBV220 to get a recombinant plasmid, which will be useful in the research of molecular targeted protein.
authors.affiliation: Institute of Medicinal Biotechnology, CAMS, Beijing.
authors.forename: M
authors.initials: M
authors.lastname: Zhang
authors.forename: X
authors.initials: X
authors.lastname: Zhao
authors.forename: H
authors.initials: H
authors.lastname: Li
authors.forename: S
authors.initials: S
authors.lastname: Lu
mesh_terms: D036002:ADP Ribose Transferases; D001427:Bacterial Toxins / Q000235:genetics* / Q000302:isolation & purification; D001483:Base Sequence; D003001:Cloning, Molecular; D005098:Exotoxins / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011550:Pseudomonas aeruginosa / Q000737:chemistry; D017422:Sequence Analysis, DNA; D037521:Virulence Factors; D000097668:Pseudomonas aeruginosa Exotoxin A
chemical_list: D036002:ADP Ribose Transferases; D001427:Bacterial Toxins; D005098:Exotoxins; D037521:Virulence Factors; D000097668:Pseudomonas aeruginosa Exotoxin A