Molecular cloning and expression of the rat homologue of presenilin-1.
The rat homologue of the presenilin-1 (PS-1) gene, which is responsible for early-onset familial Alzheimer's disease linked to chromosome 14, was cloned and sequenced. The predicted amino acid sequence showed quite high homology among rat, mouse, and human PS-1. Especially, the amino acid sequences of the putative transmembrane domains were highly conserved among the three species. The expression ĺevel of the PS-1 gene increased during brain development and the number of transcripts of the PS-1 gene changed during brain development. We found one transcript of the PS-1 gene in embryonic day 12 (E12)-E15 rat brain and two transcripts in E18-adult rat brain. Therefore, PS-1 may play a role in neurogenesis.
Takahashi
H
H
Mitsubishi Kasei Institute of Life Sciences, Tokyo, Japan.
Murayama
M
M
Takashima
A
A
Mercken
M
M
Nakazato
Y
Y
Noguchi
K
K
Imahori
K
K
D000544:Alzheimer Disease / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D002883:Chromosomes, Human, Pair 14; D003001:Cloning, Molecular; D005786:Gene Expression Regulation / Q000502:physiology*; D008040:Genetic Linkage; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D051381:Rats; D017386:Sequence Homology, Amino Acid