Antibodies to Tamm-Horsfall protein subunits prepared in vitro, in patients with acute pyelonephritis.
The aim of this study was to determine antibodies to Tamm-Horsfall protein subunits in patients with acute pyelonephritis. The protein subunits used in this determination were prepared by chemical treatment of Tamm-Horsfall protein isolated from the urine of healthy individuals. Values for IgG and IgA were significantly higher (p < 0.05 and p < 0.01 respectively) in patients than in healthy persons, while IgM class antibodies were significantly higher only in the test performed with subunits obtained with 8.3 mol/l acetic acid (THP-A) (p < 0.05). Values for all three classes determined in the test with THP-A were significantly higher in patients with vesicoureteral reflux than in patients with normal radiological findings (p < 0.05). Antibodies to Tamm-Horsfall protein subunits isolated from the urine of patients with acute pyelonephritis should also be determined.
Jelaković
B
B
Department of Medicine, University Hospital Center Zagreb, School of Medicine, University of Zagreb, Croatia.
Benković
J
J
Cikes
N
N
Kuzmanić
D
D
Roncević
T
T
Krznarić
Z
Z
D000208:Acute Disease; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007136:Immunoglobulins / Q000302:isolation & purification* / Q000652:urine; D008297:Male; D009088:Mucoproteins / Q000737:chemistry / Q000276:immunology* / Q000652:urine; D011704:Pyelonephritis / Q000175:diagnosis*; D058949:Uromodulin
D007136:Immunoglobulins; D009088:Mucoproteins; C548612:UMOD protein, human; D058949:Uromodulin