[The bioenergetic, cytochemical, and staining properties of normal hepatocytes and under functional loads, adaptations to environmental changes and in nonspecific injuries].
Two groups of cells were found among mouse liver cells and among hepatocytes in culture after their fixation and staining: weakly stained cells ("light" cells-LC), and strongly stained cells ("dark" cells- DC), the latter making about 30% of population. DC and LC do not differ in their capacity for DNA, RNA and protein synthesis, in contents of proteins, carbohydrates, lipids etc., and also in their ultrastructure. DC differ from LC in lower acidity (pH 6.5) of live cell cytoplasm, and in higher level of mitochondrial fluorescence after staining with Rhodamin 123. The live culture hepatocytes were seen in two alternative states: DC are able to become LC, and vice versa. The amount of DC increases after a high functional cell activation (partial hepatectomy, phlebotomy and hormonal stimulation), and after cell adaptation to modification of cultural medium conditions (modification of pH, hypo- and hypertonia, hyperthermia). In these cases hepatocytes maintain the capacity to granule formation after vital staining with Neutral red. After nonspecific moderate alteration (6% ethanol) of cultured hepatocytes, the acidity of cytoplasm and a temporary elevation of mitochondrial fluorescence may occur, in addition to the increase in the total number of DC. It is suggested that such a cell activation, both in normal physiological conditions and in the case of cell adaptation to modified cultural media, represents a general stress reaction of the cell preceding the "preparanecrosis" state.
Chentsov
Iu S
IuS
Kosykh
M I
MI
D000222:Adaptation, Physiological; D000818:Animals; D002478:Cells, Cultured; D004734:Energy Metabolism / Q000502:physiology*; D006651:Histocytochemistry; D006863:Hydrogen-Ion Concentration; D008099:Liver / Q000166:cytology* / Q000293:injuries* / Q000378:metabolism* / Q000502:physiology; D008751:Methylene Blue; D051379:Mice; D013194:Staining and Labeling; D013997:Time Factors
D008751:Methylene Blue
Bioénergeticheskie, tsitokhimicheskie i tinktorial'nye svoĭstva gepatotsitov v norme, pri funktsional'nykh nagruzkakh, adaptatsiiakh k izmeneniiam sredy i pri nespetsificheskikh povrezhdeniiakh.