Comparison of the canine and human acid beta-galactosidase gene.
Several canine cDNA libraries were screened with human beta-galactosidase cDNA as probe. Seven positive clones were isolated and sequenced yielding a partial (2060 bp) canine beta-galactosidase cDNA with 86% identity to the human beta-galactosidase cDNA. Preliminary analysis of a canine genomic library indicated conservation of exon number and size. Analysis by Northern blotting disclosed a single mRNA of 2.4 kb in fibroblasts and liver from normal dogs and dogs affected with GM1 gangliosidosis. Although incomplete, these results indicate canine GM1 gangliosidosis is a suitable animal model of the human disease and should further efforts to devise a gene therapy strategy for its treatment.
Ahern-Rindell
A J
AJ
Department of Zoology, Weber State University, Ogden, Utah 84408-2505, USA.
Kretz
K A
KA
O'Brien
J S
JS
D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D018076:DNA, Complementary; D004285:Dogs; D016537:Gangliosidosis, GM1 / Q000201:enzymology / Q000235:genetics*; D015698:Genomic Library; D006801:Humans; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D001616:beta-Galactosidase / Q000235:genetics* / Q000378:metabolism
D018076:DNA, Complementary; D001616:beta-Galactosidase