title: A study of the bone morphogenetic protein derived from bovine demineralized dentin matrix.
abstract: We tried to extract and purify bone morphogenetic protein (BMP) from bovine demineralized dentin matrix (DDM). Crude dentin BMP (d-BMP) was extracted from DDM in 3 sequential steps. Purification of crude d-BMP was carried out by liquid chromatography. The molecular weight and the isoelectric point of the purified d-BMP were determined by sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE) and electrofocusing. All fractioned samples were bioassayed in the thigh muscle pouches of AKR strain mice to test their ability to induce new bone formation. A sequence of 3 extraction steps worked effectively to obtain crude d-BMP. The purified d-BMP was shown to be homogeneous on high performance liquid chromatography (HPLC) and SDS-PAGE. The molecular weight and the pI were 25 kDa and 6.5, respectively. The amino acid composition was different from that of known bone-derived BMP. The purified d-BMP induced new bone formation in the thigh muscle pouches. The molecular weight, pI and amino acid composition were different from those of bone-derived BMP.
authors.affiliation: Department of Oral Surgery, Nagoya University School of Medicine, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Mizutani
authors.forename: K
authors.initials: K
authors.lastname: Mera
authors.forename: M
authors.initials: M
authors.lastname: Ueda
authors.forename: H
authors.initials: H
authors.lastname: Iwata
mesh_terms: D000818:Animals; D019485:Bone Morphogenetic Proteins / Q000302:isolation & purification*; D002417:Cattle; D003804:Dentin / Q000737:chemistry*; D008903:Minerals / Q000032:analysis
chemical_list: D019485:Bone Morphogenetic Proteins; D008903:Minerals