Lens proteins changes induced by sugars and pyridoxal phosphate.
Glucose, galactose and pyridoxal phosphate (PLP) bind to lens protein amino groups causing changes in absorbance and fluorescence spectra and inducing aggregation. Sugars and PLP simultaneously cause an increase in fluorophore and chromophore formation, but a decreased aggregation, compared to PLP alone. PLP binds to lens protein amino groups decreasing the sugar binding, but in preventing glycation by PLP attention should be paid to the consequences of its own binding to proteins in diabetes.
Ganea
E
E
Institute of Biochemistry, Bucharest, Romania.
Harding
J J
JJ
D000818:Animals; D002241:Carbohydrates / Q000502:physiology*; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D005136:Eye Proteins / Q000737:chemistry*; D005690:Galactose / Q000737:chemistry; D005947:Glucose / Q000737:chemistry; D066298:In Vitro Techniques; D007908:Lens, Crystalline / Q000737:chemistry*; D046911:Macromolecular Substances; D011485:Protein Binding; D011732:Pyridoxal Phosphate / Q000737:chemistry*
D002241:Carbohydrates; D005136:Eye Proteins; D046911:Macromolecular Substances; D011732:Pyridoxal Phosphate; D005947:Glucose; D005690:Galactose