Sequence analysis of a cDNA containing the gag and prot regions of the soybean retrovirus-like element, SIRE-1.
SIRE-1 is a family of several hundred dispersed copies of a very large DNA element from Glycine max that has features characteristic of retroviruses and retrotransposons. A 2.4 kb SIRE-1-specific fragment was recovered from a soybean cDNA library and sequenced. The sequence contains two ORFs. Theoretical translation of ORF1 produces a gag-prot-like polyprotein containing highly conserved motifs found in retroelement nucleocapsids (CX2CX4HX4C) and aspartic proteases (LDSG). The second ORF is foreshortened. The cDNA also contains nearly 200 bp of a putative 5' LTR just upstream of a tRNA primer-binding site.
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Department of Biology, Loyola University Chicago, IL 60626, USA.
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D000595:Amino Acid Sequence; D001483:Base Sequence; D018076:DNA, Complementary; D008969:Molecular Sequence Data; D018626:Retroelements; D012190:Retroviridae / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D013025:Glycine max / Q000235:genetics*
D018076:DNA, Complementary; D018626:Retroelements