title: An improved method for preparing the chromosomes of pines and other gymnosperms.
abstract: A simple technic is described to produce well spread gymnosperm chromosomes. Root tip meristems are digested with a pectinase:cellulase mixture to produce a cell suspension which then is squashed to yield flat, well spread chromosome complements that can be stained or used for in situ hybridization.
authors.affiliation: School of Biological Sciences, University of Auckland, New Zealand.
authors.forename: B G
authors.initials: BG
authors.lastname: Murray
authors.forename: B J
authors.initials: BJ
authors.lastname: Davies
mesh_terms: D002875:Chromosomes; D005821:Genetic Techniques; D014197:Trees / Q000235:genetics*