Modulation of phagocytosis by P2-purinergic receptors in mouse peritoneal macrophages.
The effects of adenosine 5'-triphosphate (ATP) and other nucleotides on phagocytosis of peritoneal macrophages were examined by means of flow cytometry (FCM). ATP and uridine 5'-triphosphate (UTP) enhanced phagocytosis in a dose-dependent manner. Control phagocytosis was partially suppressed in Ca(2+)-free solution. Phagocytosis was suppressed by ATP in Ca(2+)-free solution but not by UTP. Agonist potency order revealed that ATP enhanced phagocytosis through P2u receptors in normal external solution (NES) and inhibited it through P2z receptors in Ca(2+)-free solution. The present study showed that ATP modulates the phagocytosis of macrophages, and that two types of receptors were identified by pharmacological experiments.
Ichinose
M
M
Department of Physiology, Shimane Medical University, Izumo, Japan.
D000255:Adenosine Triphosphate / Q000494:pharmacology*; D000818:Animals; D004305:Dose-Response Relationship, Drug; D005260:Female; D005434:Flow Cytometry; D008264:Macrophages / Q000187:drug effects*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010587:Phagocytosis / Q000187:drug effects*; D011983:Receptors, Purinergic / Q000187:drug effects*; D014544:Uridine Triphosphate / Q000494:pharmacology
D011983:Receptors, Purinergic; D000255:Adenosine Triphosphate; D014544:Uridine Triphosphate