Through-bond correlation of imino and aromatic resonances in 13C-, 15N-labeled RNA via heteronuclear TOCSY.
Novel HCCNH TOCSY NMR experiments are presented that provide unambiguous assignment of the exchangeable imino proton resonances by intranucleotide through-bond connectivities to the (assigned) nonexchangeable purine H8 and pyrimidine H6 protons in uniformly 15N-, 13C-labeled RNA oligonucleotides. The HCCNH TOCSY experiments can be arranged as a two-dimensional experiment, correlating solely GH8/UH6 and GH1/UH3 proton resonances (HCCNH), 51 as three-dimensional experiments, in which additional chemical shift labeling either by GN1/UN3 (HCCNH) or by GC8/UC6 (HCCNH) chemical shifts is introduced. The utility of these experiments for the assignment of relatively large RNA oligonucleotides is demonstrated for two different RNA molecules.
Sklenár
V
V
Department of Physical and Theoretical Chemistry, Masaryk University, Brno, Czech Republic.
Dieckmann
T
T
Butcher
S E
SE
Feigon
J
J
D001482:Base Composition; D001483:Base Sequence; D002247:Carbon Isotopes; D006860:Hydrogen Bonding; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008969:Molecular Sequence Data; D009587:Nitrogen Isotopes; D009690:Nucleic Acid Conformation; D009843:Oligoribonucleotides / Q000737:chemistry*; D011687:Purines; D011743:Pyrimidines; D012313:RNA / Q000737:chemistry*
D002247:Carbon Isotopes; D009587:Nitrogen Isotopes; D009843:Oligoribonucleotides; D011687:Purines; D011743:Pyrimidines; D012313:RNA