title: Growth control of human prostatic cancer cells by the phorbol ester TPA: possible involvement of protein kinases.
abstract: To gain insights into the involvement of kinases in the growth control of prostate carcinoma cells by the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA), immunoblot analysis and in vitro phosphorylation assays were performed using kinase discriminating effectors and extracts prepared from the androgen-dependent LNCaP and the androgen-independent JCA-1 human prostate cells. The down-regulation of PKC-alpha and -beta in JCA-1 cells was correlated with the effects of TPA. In LNCaP cells, proliferation may involve DNA-PK, proposed to act possibly via phosphorylation of the androgen receptor.
authors.affiliation: Department of Urology, New York Medical College, Valhalla 10595, USA.
authors.forename: S
authors.initials: S
authors.lastname: Konno
authors.forename: T C
authors.initials: TC
authors.lastname: Hsieh
authors.forename: J M
authors.initials: JM
authors.lastname: Wu
authors.forename: Y
authors.initials: Y
authors.lastname: Chen
authors.forename: J W
authors.initials: JW
authors.lastname: Chiao
authors.forename: C
authors.initials: C
authors.lastname: Mallouh
mesh_terms: D002453:Cell Cycle / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects*; D002460:Cell Line; D051747:DNA-Activated Protein Kinase; D004268:DNA-Binding Proteins; D013196:Dihydrotestosterone / Q000494:pharmacology; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism; D007700:Kinetics; D008297:Male; D009687:Nuclear Proteins; D010750:Phosphoproteins / Q000302:isolation & purification / Q000378:metabolism; D010766:Phosphorylation; D011067:Poly dA-dT / Q000494:pharmacology; D011471:Prostatic Neoplasms; D011493:Protein Kinase C / Q000378:metabolism; D064546:Protein Kinase C beta; D051571:Protein Kinase C-alpha; D011494:Protein Kinases / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D011944:Receptors, Androgen / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
chemical_list: D004268:DNA-Binding Proteins; D007527:Isoenzymes; D009687:Nuclear Proteins; D010750:Phosphoproteins; D011944:Receptors, Androgen; D013196:Dihydrotestosterone; D011067:Poly dA-dT; D011494:Protein Kinases; D051747:DNA-Activated Protein Kinase; C497263:PRKDC protein, human; D017346:Protein Serine-Threonine Kinases; C496628:PRKCA protein, human; D011493:Protein Kinase C; D064546:Protein Kinase C beta; D051571:Protein Kinase C-alpha; D013755:Tetradecanoylphorbol Acetate