Identification of tissue-specific, dehydration-responsive elements in the Trg-31 promoter.
Trg-31 is a pea gene which is rapidly induced in response to dehydration stress. Trg-31 belongs to a family of membrane intrinsic proteins that play a role in facilitating inter-membrane transport. In order to understand the mechanism of regulation of Trg-31 expression we defined the cis-acting elements of the Trg-31 promoter and the trans-acting factors that interact with them. Deletion analysis of the Trg-31 promoter in transgenic tobacco plants indicated the presence of two positive regulatory region: (1) between the -1412 to -1066 nucleotides and (2) between the -559 to -391 nucleotides, with respect to the transcription initiation site. A negative regulatory element was also identified between -1066 and -559 nucleotides. All three regulatory regions specifically regulated Trg-31 induction in leaf tissue and had little or no effect on Trg-31 induction in root tissue. Gel-mobility shift assays indicated the presence of leaf nuclear proteins that interacted with the -1412 to -1066 and -1066 to -559 regions and an 'ABRE-like' sequence at -74 in the 5' promoter region of Trg-31.
Chaudhary
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S
CIBA Agricultural Biotechnology Research Unit, Research Triangle Park, NC 27709, USA.
Crossland
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D020346:Aquaporins; D001483:Base Sequence; D002352:Carrier Proteins; D018744:DNA, Plant; D005966:Glucuronidase / Q000235:genetics; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics*; D030821:Plants, Genetically Modified; D010947:Plants, Toxic; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D011993:Recombinant Fusion Proteins / Q000235:genetics; D012045:Regulatory Sequences, Nucleic Acid; D017384:Sequence Deletion; D014026:Nicotiana / Q000235:genetics; D014170:Transformation, Genetic; D014867:Water / Q000378:metabolism*
D020346:Aquaporins; D002352:Carrier Proteins; D018744:DNA, Plant; D009687:Nuclear Proteins; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; C080617:Trg-31 protein, Pisum sativum; D014867:Water; D005966:Glucuronidase