[The effect of GM-CSF/IL-3 on the grafting efficiency of CD34+ hemopoietic progenitor cells from umbilical cord blood].
CD34+ cells were isolated from human umbilical cord blood by using a high-gradient magnetic cell sorting system with anti-CD34 monoclonal antibody coated with microbeads, and then inoculated onto a pre-formed irradiated bone marrow stroma to evaluate the grafting efficiency of CD34+ cells with rhGM-CSF or IL-3, alone or in combination. The results showed that only 36% CD34+ cells in the control, and 68 - 89.6% in the groups with growth factors treatment seeded to the stroma. A short incubation of CD34+ cells with growth factors could rapidly reconstitute a long-term hemopoiesis in the long-term liquid culture system. This demonstrates that a brief treatment of CD34+ cells with GM-CSF or IL-3 can improve the grafting efficiency of transplanted umbilical cord blood cells.
Pei
X T
XT
Beijing Institute of Radiation Medicine.
Wu
Z Z
ZZ
Coutinho
L H
LH
Testa
N G
NG
Dexter
T M
TM
D000328:Adult; D000911:Antibodies, Monoclonal; D018952:Antigens, CD34 / Q000276:immunology*; D001853:Bone Marrow / Q000528:radiation effects; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D005312:Fetal Blood / Q000276:immunology*; D006085:Graft Survival / Q000187:drug effects; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology*; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D007377:Interleukin-3 / Q000494:pharmacology*
D000911:Antibodies, Monoclonal; D018952:Antigens, CD34; D007377:Interleukin-3; D016178:Granulocyte-Macrophage Colony-Stimulating Factor