Inhibition of hemozoin formation in Plasmodium falciparum trophozoite extracts by heme analogs: possible implication in the resistance to malaria conferred by the beta-thalassemia trait.
BACKGROUND
Human falciparum malaria, caused by the intracellular protozoa Plasmodium falciparum, results in 1-2 million deaths per year. P. falciparum digests host erythrocyte hemoglobin within its food vacuole, resulting in the release of potentially toxic free heme. A parasite-specific heme polymerization activity detoxifies the free heme by cross-linking the heme monomers to form hemozoin or malaria pigment. This biochemical process is the target of the widely successful antimalarial drug chloroquine, which is rapidly losing its effectiveness due to the spread of chloroquine resistance. We have shown that chloroquine resistance is not due to changes in the overall catalytic activity of heme polymerization or its chloroquine sensitivity. Therefore, the heme polymerization activity remains a potential target for novel antimalarials. In this study, we investigated the ability of heme analogs to inhibit heme polymerization and parasite growth in erythrocytes.


METHODS
Incorporation of radioactive hemin substrate into an insoluble hemozoin pellet was used to determine heme polymerization. Incorporation of radioactive hypoxanthine into the nucleic acid of dividing parasites was used to determine the effects of heme analogs on parasite growth. Microscopic and biochemical measurements were made to determine the extent of heme analog entry into infected erythrocytes.


RESULTS
The heme analogs tin protoporphyrin IX (SnPP), zinc protoporphyrin IX (ZnPP), and zinc deuteroporphyrin IX, 2,4 bisglycol (ZnBG) inhibited polymerization at micromolar concentrations (ZnPP << SnPP < ZnBG). However, they did not inhibit parasite growth since they failed to gain access to the site of polymerization, the parasite's food vacuole. Finally, we observed high ZnPP levels in erythrocytes from two patients with beta-thalassemia trait, which may inhibit heme polymerization.


CONCLUSIONS
The heme analogs tested were able to inhibit hemozoin formation in Plasmodium falciparum trophozite extracts. The increased ZnPP levels found in thalassemic erythrocytes suggest that these may contribute, at least in part, to the observed antimalarial protection conferred by the beta-thalassemia trait. This finding may lead to the development of new forms of antimalarial therapy.
Martiney
J A
JA
Picower Institute for Medical Research, Manhasset, NY 11030, USA.
Cerami
A
A
Slater
A F
AF
D000818:Animals; D003905:Deuteroporphyrins / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D004912:Erythrocytes / Q000469:parasitology; D006418:Heme / Q000031:analogs & derivatives / Q000378:metabolism / Q000494:pharmacology*; D006419:Heme Oxygenase (Decyclizing) / Q000037:antagonists & inhibitors; D006420:Hemeproteins / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D006801:Humans; D007113:Immunity, Innate; D007700:Kinetics; D016778:Malaria, Falciparum / Q000097:blood / Q000235:genetics / Q000276:immunology*; D008665:Metalloporphyrins / Q000494:pharmacology; D010860:Pigments, Biological; D010963:Plasmodium falciparum / Q000187:drug effects / Q000254:growth & development / Q000502:physiology*; D011524:Protoporphyrins / Q000494:pharmacology; D017086:beta-Thalassemia / Q000097:blood / Q000235:genetics*
D003905:Deuteroporphyrins; D004791:Enzyme Inhibitors; D006420:Hemeproteins; D008665:Metalloporphyrins; D010860:Pigments, Biological; D011524:Protoporphyrins; C060750:zinc deuteroporphyrin IX 2,4-bis(glycol); C017803:zinc protoporphyrin; C020753:hemozoin; D006418:Heme; C032628:tin protoporphyrin IX; D006419:Heme Oxygenase (Decyclizing)