Molecular genetics of rust resistance in poplars (Melampsora larici-populina Kleb/Populus sp.) by bulked segregant analysis in a 2 x 2 factorial mating design.
With random amplified polymorphic DNA (RAPD) markers, we have tagged a genomic region in Populus sp. involved in qualitative resistance to Melampsora larici-populina. Our approach was based on three steps: use of RAPD markers that can be quickly and efficiently researched: application of "bulked segregant analysis" technique on individuals of one interspecific family P. trichocarpa x P. deltoides to search for RAPD markers linked to resistance; and validation of these markers in two other families linked with the first one in a 2 x 2 factorial mating design. Of five detected markers, only one marker M03/04_480 was polymorphic in the three segregating families, involving 89 individuals and four different parents. We have estimated the recombination value of 1 cM with 1 cM sampling error.
Villar
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M
Institut National de la Recherche Agronomique, Station d'Amélioration des Arbres Forestiers, Ardon, France.
Lefèvre
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F
Bradshaw
H D
HD
Teissier du Cros
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E
D001487:Basidiomycota / Q000472:pathogenicity*; D003433:Crosses, Genetic; D018744:DNA, Plant / Q000032:analysis; D008040:Genetic Linkage; D005819:Genetic Markers; D007113:Immunity, Innate; D011110:Polymorphism, Genetic; D019105:Random Amplified Polymorphic DNA Technique; D011995:Recombination, Genetic; D015203:Reproducibility of Results; D014197:Trees / Q000235:genetics* / Q000382:microbiology*
D018744:DNA, Plant; D005819:Genetic Markers