Age-related changes of oxygen free radical production in the rat brain slice after hypoxia: on-line measurement using enhanced chemiluminescence.
Superoxide radical production in brain slices of 12-, 24- and 60-day-old rats was measured online during and after 15 min of hypoxia with lucigenin enhanced chemiluminescence. We found a typical radical burst after reoxygenation which developed with aging and is almost nonexistent in the youngest and most prominent in the oldest group. This cannot be explained by a decreasing tissue superoxide dismutase (SOD) concentration in the brain with aging, since the concentration of the enzyme, determined in the same age groups, increased with age.
Schreiber
S J
SJ
Department of Neurology, Humboldt University, Berlin, Germany.
Megow
D
D
Raupach
A
A
Victorov
I V
IV
Dirnagl
U
U
D000375:Aging / Q000378:metabolism*; D000704:Analysis of Variance; D000818:Animals; D001921:Brain / Q000254:growth & development / Q000378:metabolism*; D016166:Free Radical Scavengers / Q000378:metabolism*; D005609:Free Radicals; D002534:Hypoxia, Brain / Q000378:metabolism*; D066298:In Vitro Techniques; D008163:Luminescent Measurements; D008297:Male; D009862:Online Systems; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013481:Superoxides / Q000378:metabolism
D016166:Free Radical Scavengers; D005609:Free Radicals; D017382:Reactive Oxygen Species; D013481:Superoxides