Effect of pre-S1 antigen on human lymphocyte proliferative responses.
The peripheral blood mononuclear cells (PBMCs) from patients acutely infected with HBV and recovered completely (n = 20), patients with chronic hepatitis B infection (CHB)- (n = 10) and HBsAg-positive carriers (n = 9) and healthy individuals (n = 8) were studied for their in vitro proliferative response to a synthetic pre-S1(20-49)x4 antigen. PBMCs from convalescents showed significant proliferative response in the presence of synthetic pre-S1 antigen. PBMCs from CHB- and HBsAg-positive exhibited reduced proliferative response not only to Pre-S1 antigen but also to nonspecific mitogens. This study suggests that the immune recognition of pre-S1 antigen and response of PBMCs to the pre-S1 antigen may be an important part of the normal human response to HBV infection. Failure to clear the HBV infection with development of the chronic carrier state may be caused by the lack of an efficient pre-S1 antigen-specific response.
Sułowska
Z
Z
Microbiology and Virology Center, Polish Academy of Sciences, Lódź, Poland.
Dworniak
D
D
Tchórzewski
H
H
Zeman
K
K
Sidorkiewicz
M
M
D000328:Adult; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D005260:Female; D005434:Flow Cytometry; D006514:Hepatitis B Surface Antigens / Q000494:pharmacology*; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects*; D007959:Lymphocyte Culture Test, Mixed; D008297:Male; D008875:Middle Aged; D010835:Phytohemagglutinins / Q000494:pharmacology; D011498:Protein Precursors / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology; D014759:Viral Envelope Proteins / Q000276:immunology / Q000494:pharmacology*
D000911:Antibodies, Monoclonal; D006514:Hepatitis B Surface Antigens; D010835:Phytohemagglutinins; D011498:Protein Precursors; D014759:Viral Envelope Proteins; C050806:presurface protein 1, hepatitis B surface antigen