Purification of a product from Salmonella typhimurium with the ability to inhibit mitogen-induced proliferation of murine splenic T-lymphocytes.
We attempted to purify a substance that inhibits mitogen-induced proliferation of murine splenic T-lymphocytes from Salmonella typhimurium. The soluble fraction of a suspension of bacteria disrupted by sonication was chromatographed serially on Mono Q HR, Superdex 200 HR and HiLoad Superdex 75 p.g. columns. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis revealed that the purified active substance migrated as a single band corresponding to a molecular mass of 87 kDa. We designated the purified substance S. typhimurium-derived inhibitor of T-cell proliferation (STI), which, at 0.2 microgram/ml and above, inhibited proliferation and augmented CD25 expression of phytohemagglutinin-stimulated murine splenic lymphocytes. These findings suggested that the immunosuppression induced by Salmonella infection may be attributable to STI.
Matsui
K
K
Department of Microbiology, Meiji College of Pharmacy, Tokyo, Japan.
D000818:Animals; D001426:Bacterial Proteins / Q000302:isolation & purification* / Q000494:pharmacology*; D001667:Binding, Competitive / Q000276:immunology; D002478:Cells, Cultured; D007166:Immunosuppressive Agents / Q000302:isolation & purification* / Q000494:pharmacology*; D008213:Lymphocyte Activation / Q000187:drug effects*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010835:Phytohemagglutinins / Q000494:pharmacology*; D012486:Salmonella typhimurium / Q000276:immunology*; D013154:Spleen / Q000166:cytology / Q000276:immunology; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology*
D001426:Bacterial Proteins; D007166:Immunosuppressive Agents; D010835:Phytohemagglutinins