Indirect stimulatory effects of murine interleukin-12 on in vitro production of nitric oxide by mouse peritoneal cells.
The effect of murine interleukin-12 (IL-12) on L-arginine-dependent biosynthesis of nitric oxide (NO) by mouse peritoneal cells was evaluated. Interleukin-12 was found to trigger considerably enhanced production of NO in a dose-dependent manner. Antibody neutralization studies indicated that the effect of IL-12 was mediated by IFN-gamma without apparent participation of TNF-alpha. Synergistic effects of IL-12 plus lipopolysaccharide (LPS) were also observed. Our data thus provide evidence that IL-12 is a powerful but indirect modulator of NO formation. These findings may contribute to the better understanding of various biologic effects of IL-12.
Zídek
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Z
Institute of Pharmacology, Academy of Sciences of Czech Republic, Prague, Czech Republic.
Lotzová
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E
Franková
D
D
Savary
C A
CA
D000818:Animals; D002478:Cells, Cultured; D004357:Drug Synergism; D005069:Evaluation Studies as Topic; D005260:Female; D018664:Interleukin-12 / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D009569:Nitric Oxide / Q000096:biosynthesis*; D013268:Stimulation, Chemical
D008070:Lipopolysaccharides; D018664:Interleukin-12; D009569:Nitric Oxide