PAH content and mutagenicity of marine sediments from the Venice lagoon.
Sediments from the Venice lagoon, a polluted coastal environment in northeastern Italy, were assayed for mutagenicity and content of several toxic microcontaminants, which included selected polycyclic aromatic hydrocarbons (PAHs); the latter are specifically dealt with in this paper. Samples were collected at three lagoon sites with reasonably distinct environmental features--urban, industrial, or agricultural--and at two others considered to be under mixed pollution influences; a sixth sample was obtained from an open sea area to act as background control. The organic matter (EOM) associated with the mineral substrata was extracted; after cleanup, analyte determination was carried out by HRGC-LRMS(SIM) using isotopically labeled compounds as internal standards. Cumulative levels of the selected PAHs were found to be in the range of 0.065 to 0.46 micrograms/g of dry matrix at five sites; a much higher concentration (48 micrograms/g) was detected in the sample from the urban environment. The remarkable PAH level increase at this site was mostly accounted for by the concurrent, apparent increase of EOM contamination as PAH concentration was seen to reach 32 micrograms/mg of EOM from < 1 microgram/mg at the five remaining sites. Mutagenicity assays with Salmonella typhimurium strains TA98 and TA100 of marine sediment organic extracts also highlighted a distinct activity in the sample from the urban site. Further fractionation and analysis of this extract pointed to PAHs as the main mutagenic component present in the sediment matrix, possibly accounting for up to approximately 70-80% of the entire mutagenic potential detected.
La Rocca
C
C
Laboratory of Comparative Toxicology and Ecotoxicology, Instituto Superiore di Sanità, Rome, Italy.
Conti
L
L
Crebelli
R
R
Crochi
B
B
Iacovella
N
N
Rodriguez
F
F
Turrio-Baldassarri
L
L
di Domenico
A
A
D004781:Environmental Exposure; D019015:Geologic Sediments / Q000737:chemistry*; D007553:Isotope Labeling; D007558:Italy; D009152:Mutagenicity Tests; D009153:Mutagens / Q000378:metabolism / Q000633:toxicity*; D011084:Polycyclic Aromatic Hydrocarbons / Q000378:metabolism / Q000633:toxicity*; D012015:Reference Standards; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics; D012623:Seawater; D012989:Soil Pollutants / Q000378:metabolism / Q000633:toxicity*; D018629:Spectrometry, Mass, Secondary Ion; D014874:Water Pollutants, Chemical / Q000378:metabolism / Q000633:toxicity*
D009153:Mutagens; D011084:Polycyclic Aromatic Hydrocarbons; D012989:Soil Pollutants; D014874:Water Pollutants, Chemical