Effects of fluid shear stress on bone cells.
Neonatal rat calvarial cells (osteoblast-enriched) were cultured in monolayer on glass slides and subjected to a step increase in shear stress ranging from 3 to 60 dyne/cm2 in a laminar flow chamber. The level of intracellular free calcium ion concentration was monitored using Fura-2 fluorescence during the application of stress. Application of a step increase in stress resulted in a transient increase in calcium starting about 10-20 s after turning on the pump, reaching a peak in about 35 s and declining thereafter, despite the continued shear stress, and reaching near baseline values in about 100 s. No change in cytoplasmic calcium was observed with a step increase in shear stress of 3 dyne/cm2. Between 6 dyne/cm2 and 60 dyne/cm2, the magnitude of the calcium response increased with the applied shear stress.
Williams
J L
JL
Department of Orthopaedic Surgery, University of pennsylvania, Philadelphia 19104, USA.
Iannotti
J P
JP
Ham
A
A
Bleuit
J
J
Chen
J H
JH
D000818:Animals; D000831:Animals, Newborn; D001842:Bone and Bones / Q000166:cytology* / Q000502:physiology*; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D005456:Fluorescent Dyes; D016257:Fura-2; D007424:Intracellular Fluid / Q000378:metabolism; D051381:Rats; D012212:Rheology; D012886:Skull / Q000166:cytology / Q000502:physiology; D013314:Stress, Mechanical
D005456:Fluorescent Dyes; D002118:Calcium; D016257:Fura-2