title: A low-copy number plasmid mediating beta-lactamase production by Xanthomonas maltophilia.
abstract: To delineate the mechanisms contributing to the high level of antimicrobial resistance often demonstrated by Xanthomonas maltophilia, plasmid DNA was isolated from 5 clinical isolates and analyzed. Purified plasmid DNA from a single isolate contained a 6.5 kb plasmid (pXM222) and a 5.6 kb plasmid, (pTHB). Transformation of pTHB into E. coli HB101 resulted in the expression of resistance to all penicillins tested and cefazolin.
authors.affiliation: Cardeza Foundation for Hematological Research, Thomas Jefferson University, Philadelphia, PA, USA.
authors.forename: M D
authors.initials: MD
authors.lastname: Kelly
authors.forename: J E
authors.initials: JE
authors.lastname: Mortensen
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D004587:Electrophoresis, Agar Gel; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics; D008826:Microbial Sensitivity Tests; D010957:Plasmids / Q000302:isolation & purification; D015183:Restriction Mapping; D014169:Transformation, Bacterial; D014974:Xanthomonas / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D018440:beta-Lactam Resistance / Q000235:genetics*; D001618:beta-Lactamases / Q000096:biosynthesis*; D047090:beta-Lactams
chemical_list: D000900:Anti-Bacterial Agents; D047090:beta-Lactams; D001618:beta-Lactamases