Genetic probes of ribosomal RNA function.
We have used a genetic approach to uncover the functional roles of rRNA in protein synthesis. Mutations were constructed in a cloned rrn operon by site-directed mutagenesis or isolated by genetic selections following random mutagenesis. We have identified mutations that affect each step in the process of translation. The data are consistent with the results of biochemical and phylogenetic analyses but, in addition, have provided novel information on regions of rRNA not previously investigated.
O'Connor
M
M
Department of Molecular and Cellular Biology and Biochemistry, J. Wilson Laboratory, Brown University, Providence, RI 02912, USA.
Brunelli
C A
CA
Firpo
M A
MA
Gregory
S T
ST
Lieberman
K R
KR
Lodmell
J S
JS
Moine
H
H
Van Ryk
D I
DI
Dahlberg
A E
AE
D001483:Base Sequence; D008969:Molecular Sequence Data; D009154:Mutation; D009690:Nucleic Acid Conformation; D015347:RNA Probes; D012333:RNA, Messenger / Q000235:genetics; D012335:RNA, Ribosomal / Q000235:genetics*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012343:RNA, Transfer / Q000235:genetics
D015347:RNA Probes; D012333:RNA, Messenger; D012335:RNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D012343:RNA, Transfer