A p65/p95 neural surface receptor is expressed at the S-G2 phase of the cell cycle and defines distinct populations.
A surface receptor complex of Mr approximately 65 000 (p65) and approximately 95 000 (p95) is expressed in cells of the central nervous system of mice. This receptor is recognized by monoclonal antibody 87.92.6 or by reovirus type 3 haemagglutinin as unnatural ligands. The p65/p95 receptor is expressed mostly in neural embryonic precursors undergoing proliferation, especially those in the S-G2 phase of the cell cycle. Receptor expression decreases progressively throughout embryogenesis to low but detectable levels in the adult brain. Biochemical characterization revealed that the neural p65/p95 receptor complex is indistinguishable from the p65/p95 receptor expressed in T cells, where receptor ligation leads to a mitogenic block. In neural and lymphoid tissues the p65/p95 receptor (or an associated protein) possesses a tyrosine kinase enzymatic activity. Receptor ligation in neural cells resulted in the rapid tyrosine phosphorylation of cellular proteins which are different from substrates phosphorylated in T cells. Differential substrate coupling to the receptor may account for differences in signal transduction and biology between neural cells and T cells. Further study of this receptor complex may help define important features of neural proliferation, differentiation and survival.
Rebai
N
N
Department of Pharmacology and Therapeutics, McGill University, Montreal, Canada.
Almazan
G
G
Wei
L
L
Greene
M I
MI
Saragovi
H U
HU
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001921:Brain / Q000166:cytology / Q000196:embryology / Q000254:growth & development; D002135:Calcium-Binding Proteins; D002478:Cells, Cultured; D005434:Flow Cytometry; D019084:Fluorescent Antibody Technique, Indirect; D016195:G2 Phase; D018507:Gene Expression Regulation, Developmental; D008562:Membrane Glycoproteins / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D051379:Mice; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D009474:Neurons / Q000166:cytology* / Q000378:metabolism; D020794:Receptor Protein-Tyrosine Kinases / Q000378:metabolism; D011991:Receptors, Virus / Q000145:classification; D016196:S Phase; D015398:Signal Transduction; D050863:Synaptotagmin I; D050857:Synaptotagmins
D000911:Antibodies, Monoclonal; D002135:Calcium-Binding Proteins; D008562:Membrane Glycoproteins; D009419:Nerve Tissue Proteins; D011991:Receptors, Virus; D050863:Synaptotagmin I; C046176:reovirus type 3 receptor; D050857:Synaptotagmins; D020794:Receptor Protein-Tyrosine Kinases