title: Preservation of alveolar epithelial fluid transport mechanisms in rewarmed human lung after severe hypothermia.
abstract: Although hypothermia abolishes alveolar fluid clearance in the in situ goat lung and in the ex vivo human lung, it is unknown whether alveolar fluid clearance resumes in lungs that are rewarmed after severe hypothermia. An isosmolar albumin solution was instilled into resected human lungs that were rewarmed to 37 degrees C after hypothermia (7 +/- 3 degrees C), and then alveolar fluid clearance was measured by the concentration of albumin in the alveolar fluid sample after 4 h. In control experiments in lungs that had not been cooled and rewarmed, alveolar fluid clearance was 11 +/- 2% over 4 h. In separate experiments, hypothermia completely abolished alveolar fluid clearance. However, alveolar fluid clearance resumed to a normal level of 12 +/- 1% over 4 h in the lungs that were rewarmed after hypothermia. Amiloride decreased alveolar fluid clearance by 47% in the rewarmed lungs. Terbutaline increased alveolar fluid clearance by nearly 300% in 2-h experiments in the rewarmed lungs (P < 0.05). The results of this study indicate that alveolar sodium-channel transport mechanisms are preserved in resected human lungs that are exposed to rewarming after hypothermia.
authors.affiliation: Department of Surgery, Sendai Kosei Hospital, Tohoku University, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Sakuma
authors.forename: S
authors.initials: S
authors.lastname: Suzuki
authors.forename: K
authors.initials: K
authors.lastname: Usuda
authors.forename: M
authors.initials: M
authors.lastname: Handa
authors.forename: G
authors.initials: G
authors.lastname: Okaniwa
authors.forename: T
authors.initials: T
authors.lastname: Nakada
authors.forename: S
authors.initials: S
authors.lastname: Fujimura
authors.forename: M A
authors.initials: MA
authors.lastname: Matthay
mesh_terms: D000368:Aged; D004848:Epithelium / Q000502:physiology; D006801:Humans; D007035:Hypothermia / Q000503:physiopathology*; D017136:Ion Transport / Q000502:physiology; D008168:Lung / Q000187:drug effects / Q000502:physiology*; D008875:Middle Aged; D011650:Pulmonary Alveoli / Q000378:metabolism* / Q000502:physiology; D013726:Terbutaline / Q000494:pharmacology
chemical_list: D013726:Terbutaline