Butyrate-induced alteration in lipid composition of human colon cell line HT-29.
The effect of butyrate-induced differentiation on lipid composition of HT-29 cells were studied. It was observed that cell differentiation was associated with increased activity of alkaline phosphatase and changes in the lipid composition. Differentiated cells showed increased level of triacylglycerol, cholesteryl esters and decreased free cholesterol and phospholipids. Changes were also observed in individual phospholipid composition. It appears that butyrate induced differentiation is associated with increased esterification of neutral lipids.
Madesh
M
M
Wellcome Trust Research Laboratory, Department of Gastrointestinal Sciences, Christian Medical College Hospital, Vellore, India.
Benard
O
O
Balasubramaian
K A
KA
D000469:Alkaline Phosphatase / Q000378:metabolism; D002087:Butyrates / Q000494:pharmacology*; D020148:Butyric Acid; D002452:Cell Count / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D019073:HT29 Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D006801:Humans; D050356:Lipid Metabolism
D002087:Butyrates; D020148:Butyric Acid; D000469:Alkaline Phosphatase