Mapping the human Y chromosome by fingerprinting cosmid clones.
We have used Y-specific cosmid clones in a random fingerprinting approach to build contigs on the human Y chromosome. Clones derived from two libraries have been analyzed. The construction of one library is described here, the second was the Y chromosome-specific library LLOYNCO3 "M" (Lawrence Livermore National Laboratory). To date, we have fingerprinted 4430 cosmids: 377 contigs have been constructed containing from 2 to 39 clones. Along with the singletons, we estimate that we have covered 72.5% of the euchomatic portion of the Y chromosome with fingerprinted clones. Sequence tagged sites are being used to anchor cosmids and contigs onto the YAC framework.
Taylor
K
K
Galton Laboratory, University College London, UK. k.taylor@genetics.ucl.ac.uk
Hornigold
N
N
Conway
D
D
Williams
D
D
Ulinowski
Z
Z
Agochiya
M
M
Fattorini
P
P
de Jong
P
P
Little
P F
PF
Wolfe
J
J
D001483:Base Sequence; D002874:Chromosome Mapping / Q000379:methods*; D003001:Cloning, Molecular; D003360:Cosmids; D016172:DNA Fingerprinting; D017931:DNA Primers; D006801:Humans; D008969:Molecular Sequence Data; D014998:Y Chromosome
D017931:DNA Primers