title: Polymerase chain reaction-based site-directed mutagenesis using magnetic beads.
abstract: Thyroglobulin double mutants with various substitutions were obtained with a new polymerase chain reaction-based mutagenesis technique. Maximum length of megaprimer and efficiency of mutagenesis were improved by purification of single-stranded DNA, using the avidin-biotin interaction. This method might allow the construction of large libraries of DNA, mutated at different sites.
authors.affiliation: Laboratoire d'Ingénierie et Dynamique des Systèmes Membranaires, Institut de Biologie Structurale et de Microbiologie, CNRS, Marseille, France.
authors.forename: J
authors.initials: J
authors.lastname: Fieschi
authors.forename: P
authors.initials: P
authors.lastname: Niccoli
authors.forename: C
authors.initials: C
authors.lastname: Camilla
authors.forename: P
authors.initials: P
authors.lastname: Chames
authors.forename: M
authors.initials: M
authors.lastname: Chartier
authors.forename: D
authors.initials: D
authors.lastname: Baty
mesh_terms: D001360:Avidin; D001483:Base Sequence; D017931:DNA Primers; D015723:Gene Library; D008280:Magnetics; D008863:Microspheres; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D013954:Thyroglobulin / Q000235:genetics*
chemical_list: D017931:DNA Primers; D001360:Avidin; D013954:Thyroglobulin