Human liposarcoma cell line, SW872, secretes cholesteryl ester transfer protein in response to cholesterol.
Cholesteryl ester transfer protein (CETP) mediates the exchange of phospholipids and neutral lipids between the plasma lipoproteins, and plays an important role in high density lipoprotein (HDL) metabolism. While there are reports of low-level CETP secretion from cultured cells, the lack of a good model cell line has hampered the detailed study of CETP regulation and secretion. In this study, we have found that the human liposarcoma cell line, SW872, secretes cholesteryl ester transfer protein at levels substantially higher than observed from other cell lines. The secretion of CETP from this adipose-derived cell was up-regulated by 25-OH cholesterol and by low density lipoprotein (LDL) cholesterol in a concentration-dependent manner. Analysis of both full length and exon 9-deleted CETP mRNA demonstrated increases in response to LDL and 25-OH cholesterol, providing evidence for regulation at the message level. Our results suggest that the CETP-producing SW872 cell line may provide a model in which to study the regulation of this important modulator of lipoprotein metabolism.
Richardson
M A
MA
Cardiovascular Research Division, Lilly Corporate Center, Indianapolis, IN 46285-0522, USA.
Berg
D T
DT
Johnston
P A
PA
McClure
D
D
Grinnell
B W
BW
D017667:Adipocytes / Q000378:metabolism*; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D002784:Cholesterol / Q000494:pharmacology*; D053480:Cholesterol Ester Transfer Proteins; D006023:Glycoproteins / Q000378:metabolism*; D006801:Humans; D006888:Hydroxycholesterols / Q000494:pharmacology; D008077:Lipoproteins, LDL / Q000494:pharmacology; D008080:Liposarcoma; D012333:RNA, Messenger / Q000096:biosynthesis
C506642:CETP protein, human; D002352:Carrier Proteins; D053480:Cholesterol Ester Transfer Proteins; D006023:Glycoproteins; D006888:Hydroxycholesterols; D008077:Lipoproteins, LDL; D012333:RNA, Messenger; C007997:25-hydroxycholesterol; D002784:Cholesterol