title: Naturally occurring anti-idiotypic antibodies to anti-phosphotyrosine in systemic lupus erythematosus interact with SRC-homology 2 domains.
abstract: We have recently identified in SLE sera naturally occurring anti-idiotypic antibodies against anti-phosphotyrosine antibodies. Analysis of immunochemical properties of these anti-idiotypic antibodies suggest that they are of beta/gamma type mimicking the antigen. The interaction between these anti-idiotypes and SH2 domains of various fusion proteins was analysed by immunoprecipitation and immunoblotting. Our data demonstrate that these anti-idiotypic antibodies specifically bind SH2 domains, with the highest affinity for SH2 domain of lck protein tyrosine kinase. The significance of this interaction is discussed.
authors.affiliation: Department of Immunology, Cantacuzino Institute, Bucharest, Romania.
authors.forename: M
authors.initials: M
authors.lastname: Stefanescu
authors.forename: A
authors.initials: A
authors.lastname: Onu
authors.forename: C
authors.initials: C
authors.lastname: Matache
authors.forename: F
authors.initials: F
authors.lastname: Ramos-Morales
authors.forename: S
authors.initials: S
authors.lastname: Fischer
authors.forename: G
authors.initials: G
authors.lastname: Szegli
mesh_terms: D000888:Antibodies, Anti-Idiotypic / Q000502:physiology*; D019138:Antibodies, Blocking / Q000502:physiology; D000918:Antibody Specificity; D001323:Autoantibodies / Q000097:blood / Q000502:physiology*; D003429:Cross Reactions; D006801:Humans; D007113:Immunity, Innate; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*; D019860:Lymphocyte Specific Protein Tyrosine Kinase p56(lck); D019000:Phosphotyrosine / Q000276:immunology*; D018909:src Homology Domains / Q000276:immunology*; D019061:src-Family Kinases / Q000276:immunology
chemical_list: D000888:Antibodies, Anti-Idiotypic; D019138:Antibodies, Blocking; D001323:Autoantibodies; D019000:Phosphotyrosine; D019860:Lymphocyte Specific Protein Tyrosine Kinase p56(lck); D019061:src-Family Kinases