title: Immunohistologic study of the ruptured intervertebral disc of the lumbar spine.
abstract: METHODS
  This study analyzed immunohistological features of the extruded or sequestrated intervertebral disc of the lumbar spine. To clarify the pathogenesis of neovascularization, cells isolated from herniated disc were cultured and examined biologically.
  
  
  OBJECTIVE
  The objectives of this study were to characterize the histologic features of extruded or sequestrated discs and inflammatory cells that infiltrate along the margins of the disc tissue and to clarify the pathogenesis of neovascularization observed at the edge of the disc tissue.
  
  
  BACKGROUND
  When some of the contents of the disc extrudes into the epidural space and is considered "foreign," an autoimmune response develops, which can lead to a chronic inflammatory response. However, the pathogenesis of inflammatory cell infiltrations and neovascularization are not clearly defined.
  
  
  METHODS
  The herniated discs were obtained during surgery and were stained with anti-interleukin-1, intercellular adhesion molecule-1, lymphocyte function-associated antigen, and basic fibroblast growth factor antibodies by using an indirect immunoperoxidase method. Cells isolated from herniated disc were cocultured with human endothelial cells and basic fibroblast growth factor contained by cultured disc cells were measured by radioimmunoassay.
  
  
  RESULTS
  The ingrowth of granulation tissue with vascularization, occurring at the edge of fibrocartilage fragment, was present at 11 of 16 of extruded and 3 of 5 of sequestrated discs. Anti-interleukin-1, intracellular adhesion molecule-1, lymphocyte function-associated antigen, and basic fibroblast growth factor were expressed on most of mononuclear cells infiltrating into the extruded or sequestrated disc. Cells from the extruded or sequestrated disc demonstrated significantly greater levels of basic fibroblast growth factor than those from the protruded disc, and they enhanced the proliferation of endothelial cells.
  
  
  CONCLUSIONS
  This study showed that mononuclear cells infiltrating along the margins of extruded discs expressed inflammatory mediators and might induce neovascularization and persistence of inflammation.
authors.affiliation: Department of Orthopedic Surgery, Kobe University School of Medicine, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Doita
authors.forename: T
authors.initials: T
authors.lastname: Kanatani
authors.forename: T
authors.initials: T
authors.lastname: Harada
authors.forename: K
authors.initials: K
authors.lastname: Mizuno
mesh_terms: D000328:Adult; D000368:Aged; D002455:Cell Division; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism*; D004727:Endothelium / Q000166:cytology; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D007150:Immunohistochemistry; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D007375:Interleukin-1 / Q000378:metabolism; D007405:Intervertebral Disc Displacement / Q000276:immunology / Q000473:pathology*; D008159:Lumbar Vertebrae / Q000473:pathology*; D016169:Lymphocyte Function-Associated Antigen-1 / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009389:Neovascularization, Pathologic / Q000209:etiology
chemical_list: D016207:Cytokines; D007375:Interleukin-1; D016169:Lymphocyte Function-Associated Antigen-1; D016222:Fibroblast Growth Factor 2; D018799:Intercellular Adhesion Molecule-1