CREB-1 and CREB-2 immunoreactivity in the rat brain.
This study is focused to learn about the cellular localization of transcription factors binding to the cAMP response element-CREBs-in the brain of normal rats and in animals subjected to excitotoxic cell damage. For this purpose, CREB-1 and CREB-2 immunoreactivity is examined in the developing and adult rat brain under physiological conditions, and following systemic kainic acid (KA) injection at convulsant doses in the adult, as a validated experimental model of excitotoxic injury. CREB-1 immunoreactivity is constitutively expressed in periventricular glia and Bergmann glia, and appears in reactive astrocytes following KA-induced excitotoxic cell damage. In contrast, CREB-2 is constitutively expressed in all neurons of the cerebrum, cerebellum and brain stem in the developing and adult brain. CREB-2 immunoreactivity is not increased following KA excitotoxic cell damage. These results demonstrate that CREB-1 and CREB-2 in the brain of the rat are localized in separate cellular compartments and that their expression is differentially regulated in pathologic states.
Ferrer
I
I
Unitat de Neuropatologia, Hospital Princeps d'Espanya, Universitat de Barcelona, Hospitalet de Liobregat, Spain.
Blanco
R
R
Rivera
R
R
Carmona
M
M
Ballabriga
J
J
Olivé
M
M
Planas
A M
AM
D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000254:growth & development / Q000473:pathology; D001923:Brain Chemistry / Q000502:physiology*; D017362:Cyclic AMP Response Element-Binding Protein / Q000032:analysis*; D005260:Female; D007150:Immunohistochemistry; D008297:Male; D009419:Nerve Tissue Proteins / Q000032:analysis*; D011257:Pregnancy Proteins / Q000032:analysis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results
D017362:Cyclic AMP Response Element-Binding Protein; D009419:Nerve Tissue Proteins; D011257:Pregnancy Proteins