title: Expression of Na+/myo-inositol cotransporter mRNA in normal and hypertonic stress rat eyes.
abstract: We studied the localization of Na+/myo-inositol cotransporter (SMIT) mRNA in normal and hypertonic stress rat eyes by in situ hybridization histochemistry using cRNA probes. SMIT mRNA signals were observed in the iris-ciliary body, the lens epithelial cells, and the ganglion cell layer and the inner nuclear layer of the retina. There was a rapid increase on SMIT mRNA in the retina of hypertonic stress rats compared with control rats. These findings suggest that Na+/myo-inositol cotransporter gene expression is osmotically regulated in vivo to protect retinal neuronal function against hypertonic stress.
authors.affiliation: Department of Ophthalmology, Osaka University Medical School, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Morimura
authors.forename: S
authors.initials: S
authors.lastname: Shimada
authors.forename: Y
authors.initials: Y
authors.lastname: Otori
authors.forename: A
authors.initials: A
authors.lastname: Yamauchi
authors.forename: Y
authors.initials: Y
authors.lastname: Minami
authors.forename: K
authors.initials: K
authors.lastname: Inoue
authors.forename: A
authors.initials: A
authors.lastname: Miyai
authors.forename: I
authors.initials: I
authors.lastname: Ishimoto
authors.forename: Y
authors.initials: Y
authors.lastname: Tano
authors.forename: M
authors.initials: M
authors.lastname: Tohyama
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000096:biosynthesis*; D002924:Ciliary Body / Q000378:metabolism; D005123:Eye / Q000378:metabolism*; D015870:Gene Expression; D006360:Heat-Shock Proteins / Q000096:biosynthesis*; D017403:In Situ Hybridization; D007294:Inositol / Q000378:metabolism; D007908:Lens, Crystalline / Q000378:metabolism; D008297:Male; D008565:Membrane Proteins; D015347:RNA Probes; D018075:RNA, Complementary; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar; D012016:Reference Values; D012160:Retina / Q000378:metabolism; D012165:Retinal Ganglion Cells / Q000378:metabolism; D012462:Saline Solution, Hypertonic; D012964:Sodium / Q000378:metabolism; D013312:Stress, Physiological; D027981:Symporters
chemical_list: D002352:Carrier Proteins; D006360:Heat-Shock Proteins; D008565:Membrane Proteins; D015347:RNA Probes; D018075:RNA, Complementary; D012333:RNA, Messenger; D012462:Saline Solution, Hypertonic; D027981:Symporters; C073884:SLC5A3 protein, human; D007294:Inositol; D012964:Sodium