Allele imbalance at tumour suppressor loci during the indolent phase of follicle centre cell lymphoma.
We have examined 41 cases of follicle centre cell lymphoma with fluorescent PCR of microsatellite repeats closely linked to or within six tumour suppressor gene loci (APC, DCC, P53, RB1, WT1 and NM23). These probes are highly informative with heterozygousity rates in the range of 57%-90%. In addition we have used four loci from chromosome 6 (D6S260, TNFa, D6S281 and D6S262) as control loci which are unlikely to be involved in the pathogenesis of lymphoma. Of 369 informative PCR reactions allele imbalance was identified in 38 (10%) and this was seen in 23 of the 41 cases. Looking at individual loci allele imbalance was seen in APC(1) 11%, APC(2) 12%, P53(1) 5%, P53 (2) 7%, WT1 5%, RB1 13%, DCC 18% and NM23 0%. This frequency of change was no different from that seen at the control loci D6S260 16%, TNFa 20%, D6S281 4% and D6S262 9%. In the indolent phase of germinal centre cell lymphoma there is therefore quite a high rate of allele imbalance at all loci but this is no higher in those loci linked to tumour suppressor genes.
Randerson
J
J
Institute of Pathology, University of Leeds, West Yorkshire, England, UK.
Cawkwell
L
L
Jack
A S
AS
Child
A J
AJ
Shiach
C R
CR
Lewis
F
F
Johnson
P
P
Evans
P
P
Barrans
S
S
Morgan
G J
GJ
D000483:Alleles; D002883:Chromosomes, Human, Pair 14 / Q000648:ultrastructure; D002887:Chromosomes, Human, Pair 18 / Q000648:ultrastructure; D002896:Chromosomes, Human, Pair 6 / Q000235:genetics; D004273:DNA, Neoplasm / Q000235:genetics*; D018450:Disease Progression; D017353:Gene Deletion; D016147:Genes, Tumor Suppressor; D006801:Humans; D008224:Lymphoma, Follicular / Q000235:genetics* / Q000473:pathology; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction; D014178:Translocation, Genetic
D004273:DNA, Neoplasm