title: Effect of conditions in obtaining blood samples for ECP testing in children.
abstract: Eosinophil Cationic Protein (ECP) is a basic protein found in eosinophil granules. This cell and its mediators are currently considered to be potential indicators of the severity of inflammation in the organism. ECP concentration can be reliably tested using several RIA or ELISA methods. It is well known that the conditions of sample obtention can affect the ECP values in blood. The aim of this study is to establish which parameters affect ECP testing during regular blood sample collection and how they affect it. Blood samples taken for the routine study of five children attended in our department were analysed: four were asthmatic and one child had atopic dermatitis. In the results we observed that ECP was not detected in the blood samples taken with EDTA tripotassium. In both the plasma samples taken with heparin as well as with serum, more ECP was released at a higher temperature. In the release of ECP obtained by coagulation, samples at 37 degrees showed values of between 4 and 20 higher than those obtained for an hour at 0 degrees. There is a considerable variability in the testing of ECP depending on the blood test extraction conditions, the range is bigger in the samples with eosinophils. These results imply the need to define a stricter protocol for obtaining samples than that suggested at present.
authors.affiliation: Biochemical, Allergy Department, Voll d'Hebron Maternity-Children's University Hospital.
authors.forename: J M
authors.initials: JM
authors.lastname: Pena
authors.forename: N
authors.initials: N
authors.lastname: Rubira
authors.forename: J
authors.initials: J
authors.lastname: Botey
authors.forename: M J
authors.initials: MJ
authors.lastname: Rodrigo
authors.forename: R
authors.initials: R
authors.lastname: Alonso
authors.forename: J L
authors.initials: JL
authors.lastname: Eseverri
authors.forename: A
authors.initials: A
authors.lastname: Marín
authors.forename: R M
authors.initials: RM
authors.lastname: Ras
mesh_terms: D000293:Adolescent; D000925:Anticoagulants / Q000494:pharmacology; D001249:Asthma / Q000097:blood; D001798:Blood Proteins / Q000032:analysis*; D001800:Blood Specimen Collection / Q000379:methods; D002648:Child; D002675:Child, Preschool; D003876:Dermatitis, Atopic / Q000097:blood; D004492:Edetic Acid / Q000494:pharmacology; D047091:Eosinophil Granule Proteins; D004804:Eosinophils / Q000737:chemistry; D005188:False Negative Reactions; D005260:Female; D006493:Heparin / Q000494:pharmacology; D006801:Humans; D007958:Leukocyte Count; D008297:Male; D015203:Reproducibility of Results; D012260:Ribonucleases; D013696:Temperature; D013997:Time Factors
chemical_list: D000925:Anticoagulants; D001798:Blood Proteins; D047091:Eosinophil Granule Proteins; D006493:Heparin; D004492:Edetic Acid; D012260:Ribonucleases