cDNA encoding a wheat (Triticum aestivum cv. Chinese spring) glycine-rich RNA-binding protein.
A wheat cDNA encoding a glycine-rich RNA-binding protein, whGRP-1, was isolated. WhGRP-1 contains two conserved domains, the RNA-binding motif (RNP motif) combined with a series of glycine-rich imperfect repeats, characteristic of a conserved family of plant RNA-binding proteins. Northern analysis revealed that whGRP-1 mRNA accumulates to high levels in roots and to lower levels in leaves of wheat seedlings, whGRP-1 mRNA accumulation is not enhanced by exogenous abscisic acid in seedlings and accumulates to very high levels during wheat embryo development, showing a pattern different from that of the ABA-inducible wheat Em gene.
Guiltinan
M J
MJ
Department of Horticulture, Pennsylvania State University, University Park 16802, USA.
Niu
X
X
D000040:Abscisic Acid / Q000494:pharmacology; D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D018076:DNA, Complementary; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D014908:Triticum / Q000196:embryology / Q000235:genetics* / Q000254:growth & development
D018076:DNA, Complementary; D010940:Plant Proteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; C100791:glycine-rich RNA-binding protein, plant; D000040:Abscisic Acid