Glycan structure of a heptose-containing S-layer glycoprotein of Bacillus thermoaerophilus.
The characterization of the S-layer glycoprotein of Bacillus thermoaerophilus revealed unexpected novelties. The isolation and purification procedure had to be changed due to complete solubility in aqueous buffers of the constituting S-layer protomers. Upon degradation of the S-layer glycoprotein by pronase and purification of the products by gel filtration, ion-exchange chromatography, chromatofocusing and HPLC, one representative glycopeptide fraction was selected for further characterization. From the combined evidence of composition analysis, chemical degradation, NMR spectroscopy experiments and comparison with synthesized model substance, we propose the following repeating unit structure of the glycan chain: -->4)-alpha-L-Rhap-(1-->3)-beta-D-glycero-D-manno-Hepp-(1--> This is the first description of heptose residues occurring as a constituent of S-layer glycoproteins of gram-positive eubacteria.
Kosma
P
P
Zentrum fur Ultrastrukturforschung und Ludwig Boltzmann-Institut fur Molekulare Nanotechnologie, Wien, Austria.
Wugeditsch
T
T
Christian
R
R
Zayni
S
S
Messner
P
P
D001407:Bacillus / Q000737:chemistry*; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry*; D001426:Bacterial Proteins; D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D004187:Disaccharides / Q000737:chemistry; D006020:Glycopeptides / Q000737:chemistry; D006023:Glycoproteins / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008356:Mannoheptulose / Q000032:analysis*; D008562:Membrane Glycoproteins; D008969:Molecular Sequence Data; D017421:Sequence Analysis
D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D004187:Disaccharides; D006020:Glycopeptides; D006023:Glycoproteins; D008562:Membrane Glycoproteins; C055764:S-layer proteins; D008356:Mannoheptulose