Cholinergic inhibition of muscle fibres isolated from Schistosoma mansoni (Trematoda:Digenea).
Cholinergic compounds inhibit FMRFamide-induced contractions in dispersed muscle fibres isolated from adult Schistosoma mansoni. Acetylcholine (ACh) was the most effective cholinergic agonist tested with an EC50 < 100 nM. Less effective were propionylcholine and arecoline with EC50 < 1 microM and butyrylcholine and carbachol with EC50 < 10 microM. Choline, muscarine, pilocarpine, nicotine, DMPP (1,1-dimethylphenylpiperazine) and levamisole were all ineffective. Amongst tested antagonists, d-tubocurarine (100 microM), mecamylamine (1 mM), scopolamine (1 mM) and quinuclidinyl benzilate (10 microM) were all ineffective. Bicuculline, picrotoxin and strychnine were also ineffective. However alpha-bungarotoxin, at 100 nM, was able to block the inhibitory ACh effect. From these data it appears that the cholinergic receptor on the schistosome muscle fibres may be of the nicotinic type, but that its pharmacology is different from that of nicotinic receptors of vertebrates as well as of nematodes or a variety of other invertebrates.
Day
T A
TA
Department of Pharmacology and Toxicology, Michigan State University, East Lansing 48824, USA.
Chen
G Z
GZ
Miller
C
C
Tian
M
M
Bennett
J L
JL
Pax
R A
RA
D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D018679:Cholinergic Agonists / Q000494:pharmacology*; D018680:Cholinergic Antagonists / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D019835:FMRFamide; D009119:Muscle Contraction / Q000187:drug effects; D018485:Muscle Fibers, Skeletal / Q000187:drug effects*; D009479:Neuropeptides / Q000494:pharmacology; D018377:Neurotransmitter Agents / Q000494:pharmacology; D011950:Receptors, Cholinergic / Q000187:drug effects / Q000378:metabolism; D012550:Schistosoma mansoni / Q000187:drug effects* / Q000502:physiology
D018679:Cholinergic Agonists; D018680:Cholinergic Antagonists; D009479:Neuropeptides; D018377:Neurotransmitter Agents; D011950:Receptors, Cholinergic; D019835:FMRFamide; D000109:Acetylcholine