The action of phenazine methosulphate in causing cellular damage in the isolated mouse soleus muscle preparation.
Exposure of the isolated mouse soleus preparation to 1.0 mM phenazine methosulphate (PMS) caused (i) a slow and modest release of creatine kinase (CK) that was exacerbated by removal of extracellular Ca2+, (ii) a specific type of ultrastructural damage, namely a characteristic spacing of the myofibrils, (iii) swelling of the mitochondria, indicating a modest rise in [Ca2+]i, and (iv) swelling of the sarcoplasmic reticulum (SR). It is suggested that PMS (i) activates a sarcolemma oxidoreductase which synergistically interacts with raised [Ca2+]i to cause modest CK efflux and (ii) activates an oxidoreductase on the SR, thereby generating electrons which directly modify the integrity and organisation of the contractile apparatus.
McCall
K E
KE
Department of Zoology, School of Life Sciences, University of Liverpool, UK.
Duncan
C J
CJ
D000818:Animals; D003402:Creatine Kinase / Q000187:drug effects / Q000378:metabolism; D066298:In Vitro Techniques; D008773:Methylphenazonium Methosulfate / Q000633:toxicity*; D051379:Mice; D008807:Mice, Inbred BALB C; D018482:Muscle, Skeletal / Q000187:drug effects* / Q000201:enzymology / Q000648:ultrastructure; D009336:Necrosis
D008773:Methylphenazonium Methosulfate; D003402:Creatine Kinase