[Regenerating hematopoietic tissue as a source for a low molecular weight factor that inhibits leukemic cell proliferation].
Using a successive set of chromatographic methods, a low molecular weight factor inhibiting leukemic cells proliferation has been isolated from an aqueous extract of regenerating hematopoietic tissue. This factor inhibits DNA synthesis in leukemic cells about 1000-fold more effectively than the original extract. The factor activity does not change after treatment by proteinase K; however, successive action of endo-alpha-N-acetylgalactose aminidase and proteinase K significantly decreases the efficiency of the factor action. This factor whose molecular mass is less than 3 kDa seems to be of glycopeptide origin.
Sheleg
S V
SV
Svirnovskiĭ
A I
AI
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001853:Bone Marrow / Q000378:metabolism* / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D019286:Endopeptidase K / Q000378:metabolism; D006131:Growth Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000502:physiology*; D015850:Interleukin-6; D053605:Leukemia Inhibitory Factor; D007942:Leukemia, Experimental / Q000473:pathology*; D008222:Lymphokines / Q000737:chemistry / Q000302:isolation & purification / Q000502:physiology*; D051379:Mice; D008970:Molecular Weight; D012038:Regeneration
D000596:Amino Acids; D006131:Growth Inhibitors; D015850:Interleukin-6; D053605:Leukemia Inhibitory Factor; C507838:Lif protein, mouse; D008222:Lymphokines; D019286:Endopeptidase K
Regeneriruiushchaia krovetvornaia tkan' kak istochnik polucheniia nizkomolekuliarnogo faktora, ingibiruiushchego proliferatsiiu leĭkoznykh kletok.