[Relation between loss of heterozygosity at APC and MCC genetic loci and biological behaviour of gastric carcinoma].
In order to detect loss of heterozygosity (LOH) at APC and MCC genetic loci in gastric carcinoma, the authors established a micro-wax-mediated hot start PCR technique. This method allowed a specific gene amplification, and it was useful especially in the amplification of formalin-fixed or stained tissues. In 44 cases of gastric cancer, 29 cases were informative of the APC locus. LOH was found in 8 cases (27.6%): 2 cases in 2 moderately well-differentiated cancer, 2 cases in 13 differentiated cancer, and 4 cases in poorly-differentiated cancer. One of the 3 cases of gastric cancer at early stage also showed LOH. LOH at MCC locus was detected in only 2 of the 25 (8.0%) gastric cancer patients informative. These data suggest that abnormality of APC gene plays a role in the tumorigenesis of gastric cancer and the change may occur at the early stage of tumor development.
Liu
B
B
Center of Gastroenterology, Xijing Hospital, Fourth Military Medical University, Xi'an.
Zhang
X
X
Yu
W
W
D000230:Adenocarcinoma / Q000235:genetics*; D017353:Gene Deletion; D017491:Genes, APC / Q000235:genetics*; D017431:Genes, MCC / Q000235:genetics*; D006579:Heterozygote; D006801:Humans; D013274:Stomach Neoplasms / Q000235:genetics*