title: Hormonal regulation of plasminogen activator in rat and mouse seminiferous epithelium.
abstract: To elucidate the possible role of plasminogen activator (PA) in spermatogenesis and spermiation in mammals, we studied the hormonal regulation of PA secretion in cultured rat and mouse seminiferous tubules during defined stages of spermatogenesis. Results indicated that: (1) under basal conditions, segments of rat seminiferous tubules released primarily urokinase-type PA (uPA) at all stages of the cycle. The highest level of PA secretion occurred at stages VIIab, VIIcd and VIII. FSH, 8-bromo cyclic AMP and forskolin (FK) stimulated PA secretion, predominantly tissue-type PA (tPA). (2) In contrast, mouse seminiferous tubules secreted only tPA under basal conditions. In the presence of 50 microM MIX, seminiferous tubules at stages VII and VIII secreted higher levels of both types of PA than at the other stages. Both tPA and uPA secretion was enhanced by addition of FSH and FK to the organ culture media. (3) Segments of both rat and mouse seminiferous tubules at stages IX-XII in which the sperm residual bodies are absorbed into the Sertoli cells were also very sensitive to the addition of FSH to the organ culture. These results suggest that tPA in rat and mouse testes may play an essential role in the process of spermatogenesis and spermiation as well as in sperm residual body absorption.
authors.affiliation: Institute of Zoology, Chinese Academy of Sciences, Beijing, People's Republic of China.
authors.forename: Y X
authors.initials: YX
authors.lastname: Liu
authors.forename: Q
authors.initials: Q
authors.lastname: Du
authors.forename: K
authors.initials: K
authors.lastname: Liu
authors.forename: G Q
authors.initials: GQ
authors.lastname: Fu
mesh_terms: D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000494:pharmacology; D000818:Animals; D005576:Colforsin / Q000494:pharmacology; D004848:Epithelium / Q000187:drug effects / Q000201:enzymology; D005640:Follicle Stimulating Hormone / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009924:Organ Culture Techniques; D051381:Rats; D017207:Rats, Sprague-Dawley; D012671:Seminiferous Tubules / Q000201:enzymology* / Q000502:physiology; D012708:Sertoli Cells / Q000187:drug effects / Q000201:enzymology; D010959:Tissue Plasminogen Activator / Q000378:metabolism*; D014568:Urokinase-Type Plasminogen Activator / Q000378:metabolism*
chemical_list: D005576:Colforsin; D015124:8-Bromo Cyclic Adenosine Monophosphate; D005640:Follicle Stimulating Hormone; D010959:Tissue Plasminogen Activator; D014568:Urokinase-Type Plasminogen Activator; D015056:1-Methyl-3-isobutylxanthine