Merocyanine 540 mediated photoirradiation of leukemic cells. In vitro inference on cell survival.
In order to evaluate the selective killing of merocyanine 540 (MC 540) mediated photoirradiation in neoplastic cells, bone narrow cells from children with leukaemia or neuroblastoma and normal children as well as peripheral blood cells and Reh-6 and HL-60 cell lines were studied. Cell suspensions were incubated with MC 540 and exposed to various argon laser 514 nm doses. Cell survival was estimated with trypan blue supravital stain following a 24 h incubation and has been followed in continuous cell cultures of 4 weeks duration. Our results showed that the inhibition of survival of neoplastic haemopoietic cells by laser in the presence of MC 540 is proportional to the MC 540 and photoirradiation doses. A 99.9999% inhibition of Reh-6 and HL-60 was noted at irradiation doses where the corresponding mean survival of normal bone narrow cells was (33.6 +/- 15.5)% and (50.6 +/- 10.7)% respectively. Peripheral blood mononuclear cells were not sensitive to MC 540 mediated photoirradiation. The inhibition of survival of bone marrow metastatic neuroblastoma cells was (69.9 +/- 4.1)%. In conclusion, it seems that MC 540 mediated photoirradiation in neoplastic cells exerts selective cytotoxicity and can be used in ex vivo purging of malignant cells in the bone marrow.
Lydaki
E
E
Department of Pediatric Hematology-Oncology, University of Crete Medical School, Greece.
Dimitriou
H
H
Papazoglou
T
T
Bolonaki
E
E
Kalmanti
M
M
D001853:Bone Marrow / Q000473:pathology; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects* / Q000528:radiation effects; D002648:Child; D004307:Dose-Response Relationship, Radiation; D018922:HL-60 Cells; D006412:Hematopoietic Stem Cells / Q000187:drug effects* / Q000473:pathology / Q000528:radiation effects; D006801:Humans; D007700:Kinetics; D007938:Leukemia / Q000473:pathology; D008027:Light; D009447:Neuroblastoma; D017319:Photosensitizing Agents / Q000633:toxicity*; D011744:Pyrimidinones / Q000633:toxicity*; D014407:Tumor Cells, Cultured
D017319:Photosensitizing Agents; D011744:Pyrimidinones; C003954:merocyanine dye