title: [Reactions of decarboxylated and side transamination during interaction of glutamate decarboxylase from Escherichia coli with substrate analogs, modified through C3 and C4 atoms].
abstract: The interaction of glutamate decarboxylase with the aspartate and glutamate analogues modified at C3 and C4 was studied. 3-Arsonoalanine, 3-phosphonoalanine, 2-amino-4-arsonobutyric acid, 2-amino-4-phosphonobutyric acid, a mixture of diastereoisomers of 4-(methylthio) glutamic acid and erythro-4-(methylthio) glutamic acid were shown to be poor substrates for the enzyme. Their decarboxylation was accompanied by transamination of the coenzyme (PLP) to pyridoxamine phosphate (PMP) which reversibly inactivated the enzyme. With arsonoalanine only part of PLP was converted into PMP and another part irreversibly formed a complex. 4-(Methylsulfonyl)-L-glutamic and 4-[(phenyl)(hydroxy)phosphoryl]-L-glutamic acids did not react with the glutamate decarboxylase.
authors.affiliation: Department of Biochemistry, University of Cambridge, UK.
authors.forename: R R
authors.initials: RR
authors.lastname: Khristoforov
authors.forename: B S
authors.initials: BS
authors.lastname: Sukhareva
authors.forename: H B
authors.initials: HB
authors.lastname: Dixon
authors.forename: M J
authors.initials: MJ
authors.lastname: Sparkes
authors.forename: V P
authors.initials: VP
authors.lastname: Krasnov
authors.forename: I M
authors.initials: IM
authors.lastname: Bukrina
authors.forename: A N
authors.initials: AN
authors.lastname: Grishakov
mesh_terms: D000586:Amination; D002244:Carbon / Q000737:chemistry*; D002264:Carboxylic Acids / Q000737:chemistry; D002942:Circular Dichroism; D004926:Escherichia coli / Q000201:enzymology*; D005968:Glutamate Decarboxylase / Q000737:chemistry* / Q000378:metabolism; D011732:Pyridoxal Phosphate / Q000737:chemistry; D011733:Pyridoxamine / Q000031:analogs & derivatives / Q000737:chemistry; D013237:Stereoisomerism; D013379:Substrate Specificity
chemical_list: D002264:Carboxylic Acids; D011732:Pyridoxal Phosphate; D011733:Pyridoxamine; D002244:Carbon; D005968:Glutamate Decarboxylase; C010627:pyridoxamine phosphate
vernacular_title: Reaktsii dekarboksilirovaniia i pobochnogo transaminirovaniia pri vzaimodeĭstvii glutamatdekarboksilazy iz Escherichia coli s analogami substrata, modifitsirovannymi po atomam C3 i C4.