title: Docosahexaenoic acid modulates the interactions of the interphotoreceptor retinoid-binding protein with 11-cis-retinal.
abstract: Rapid transport of retinoids across the interphotoreceptor matrix is a critical part of the visual cycle, since it serves to replenish bleached rhodopsin with its chromophore 11-cis-retinal. The transport of retinoids in the interphotoreceptor matrix is believed to be mediated by the interphotoreceptor retinoid-binding protein (IRBP), a protein that, in addition to possessing two retinoid-binding sites, associates in vivo with long chain fatty acids. Here, the interrelationships between binding of the two types of ligands to IRBP were studied. The composition of fatty acids associated with IRBP in bovine retina was determined, and it was found that polyunsaturated fatty acids constitute a significant fraction of those. It was further found that docosahexaenoic acid, but not palmitic acid, induced a rapid and specific release of 11-cis-retinal from one of the protein's retinoid-binding sites. Based on these results and on the additional observation that a steep concentration gradient of docosahexaenoic acid exists between photoreceptor and pigment epithelium cells, a model for the mechanism by which IRBP may target 11-cis-retinal to photoreceptor cells is proposed.
authors.affiliation: Division of Nutritional Sciences, Savage Hall, Cornell University, Ithaca, New York 14853-6301, USA.
authors.forename: Y
authors.initials: Y
authors.lastname: Chen
authors.forename: L A
authors.initials: LA
authors.lastname: Houghton
authors.forename: J T
authors.initials: JT
authors.lastname: Brenna
authors.forename: N
authors.initials: N
authors.lastname: Noy
mesh_terms: D000494:Allosteric Regulation; D000818:Animals; D001692:Biological Transport; D002417:Cattle; D002451:Cell Compartmentation; D004281:Docosahexaenoic Acids / Q000378:metabolism*; D005136:Eye Proteins; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000378:metabolism; D010713:Phosphatidylcholines / Q000737:chemistry; D010857:Pigment Epithelium of Eye / Q000378:metabolism; D011485:Protein Binding; D012172:Retinaldehyde / Q000378:metabolism*; D012177:Retinol-Binding Proteins / Q000378:metabolism*; D012374:Rod Cell Outer Segment / Q000378:metabolism; D012995:Solubility; D013050:Spectrometry, Fluorescence; D013237:Stereoisomerism; D014801:Vitamin A / Q000737:chemistry / Q000378:metabolism; D000099214:Retinol-Binding Proteins, Interstitial
chemical_list: D004281:Docosahexaenoic Acids; D005136:Eye Proteins; D019308:Palmitic Acid; D010169:Palmitic Acids; D010713:Phosphatidylcholines; D012172:Retinaldehyde; D012177:Retinol-Binding Proteins; D014801:Vitamin A; D000099214:Retinol-Binding Proteins, Interstitial; C017251:1,2-oleoylphosphatidylcholine