title: Amitraz effects on foot-and-mouth disease virus in mammalian cells in vitro.
abstract: The toxicity of the acaricide amitraz and its effect on foot-and-mouth disease virus multiplication were evaluated in IB-RS-2 cells in vitro. A reduction of cell growth rate that was dependent on the dose and the length of treatment was observed in cells exposed to amitraz concentrations ranging from 20 to 50 micrograms/ml. Foot-and-mouth disease virus infectivity remained essentially unchanged in cells exposed to amitraz (20 micrograms/ml) 24 hr prior to virus infection or after the adsorption period. Viral RNA synthesis evaluated through [3H]uridine incorporation in cells treated for 24 hr prior to infection was not affected by amitraz.
authors.affiliation: Instituto Biológico, Säo Paulo, Brazil.
authors.forename: M
authors.initials: M
authors.lastname: D'Angelo
authors.forename: M A
authors.initials: MA
authors.lastname: Rodrigues
mesh_terms: D000818:Animals; D005537:Aphthovirus / Q000187:drug effects*; D002452:Cell Count / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002869:Chromosome Aberrations / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D007306:Insecticides / Q000633:toxicity*; D007668:Kidney / Q000166:cytology / Q000187:drug effects* / Q000473:pathology; D008322:Mammals; D012367:RNA, Viral / Q000187:drug effects / Q000378:metabolism; D014052:Toluidines / Q000494:pharmacology / Q000633:toxicity*; D014529:Uridine / Q000378:metabolism; D014779:Virus Replication / Q000187:drug effects
chemical_list: D007306:Insecticides; D012367:RNA, Viral; D014052:Toluidines; C014983:amitraz; D014529:Uridine