Induction of PGH synthase and c-fos mRNA during early reperfusion of ischemic rat brain.
We examined the effect of reversible ischemia on the transcription of prostaglandin endoperoxide synthase (PGHS-1) and c-fos mRNA in rat cerebral cortex. The level of PGHS-1 mRNA climaxed after 30 min of ischemia whereas transcription of c-fos mRNA peaked after 60 min of postischemic reperfusion. We conclude that cerebral ischemia causes early transcription of PGHS-1, without modulation by the c-fos gene or its translated product.
Holtz
M L
ML
Stroke Program of the Sanders-Brown Center of Excellence in Aging, University of Kentucky, College of Medicine, Lexington 40536-0230, USA.
Kindy
M S
MS
Craddock
S
S
Moore
R W
RW
Pettigrew
L C
LC
D000818:Animals; D015152:Blotting, Northern; D001921:Brain / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic; D016762:Genes, fos; D002546:Ischemic Attack, Transient / Q000378:metabolism*; D008297:Male; D011451:Prostaglandin-Endoperoxide Synthases / Q000096:biosynthesis*; D016760:Proto-Oncogene Proteins c-fos / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar; D015424:Reperfusion; D013997:Time Factors; D014158:Transcription, Genetic
D016760:Proto-Oncogene Proteins c-fos; D012333:RNA, Messenger; D011451:Prostaglandin-Endoperoxide Synthases