title: Stability and oligomeric equilibria of refolded interleukin-1beta converting enzyme.
abstract: We report the preparation and characterization of interleukin-1beta converting enzyme (ICE) refolded from its p20 and p10 protein fragments. Refolded ICE heterodimer (p20p10) was catalytically active but unstable, and in size exclusion chromatography eluted at an apparent molecular mass of 30 kDa. The mechanisms of the observed instability were pH-dependent dissociation at low enzyme concentrations, and autolytic degradation of the p10 subunit at high concentrations. Binding and subsequent removal of a high affinity peptidic inhibitor increased the apparent molecular mass to 43 kDa (by size exclusion chromatography), and significantly increased its stability and specific activity. Chemical cross-linking and SDS-polyacrylamide gel electrophoresis analysis of the 43-kDa size exclusion chromatography conformer revealed a 60-kDa species, which was absent in the 30-kDa conformer, suggesting that inhibitor binding caused formation of a (p20p10)2 homodimer. The observation of a reversible equilibrium between ICE (p20p10) and (p20p10)2 suggests that analogous associations, possibly between ICE and ICE homologs, can occur in vivo, resulting in novel oligomeric protease species.
authors.affiliation: BASF Bioresearch Corporation, Worcester, Massachusetts 01605, USA.
authors.forename: R V
authors.initials: RV
authors.lastname: Talanian
authors.forename: L C
authors.initials: LC
authors.lastname: Dang
authors.forename: C R
authors.initials: CR
authors.lastname: Ferenz
authors.forename: M C
authors.initials: MC
authors.lastname: Hackett
authors.forename: J A
authors.initials: JA
authors.lastname: Mankovich
authors.forename: J P
authors.initials: JP
authors.lastname: Welch
authors.forename: W W
authors.initials: WW
authors.lastname: Wong
authors.forename: K D
authors.initials: KD
authors.lastname: Brady
mesh_terms: D001483:Base Sequence; D020170:Caspase 1; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D003546:Cysteine Endopeptidases / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D004795:Enzyme Stability; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016133:Polymerase Chain Reaction; D011487:Protein Conformation; D017434:Protein Structure, Tertiary
chemical_list: D003546:Cysteine Endopeptidases; D020170:Caspase 1