Rapid and sensitive screening of antifungal activity in medicinal plants by a single-cell biosensing system.
A biosensing system based on the response of fungal cells was used for the evaluation of antifungal activity of medicinal plants against Aspergillus niger. This system measured the hyphal growth rate in real time in the presence or absence of Chinese herbal extracts. The sensitivity of this system was 100-fold higher than that of conventional methods, and is advisable for the screening of antifungal compounds.
Oh
K B
KB
Department of Biotechnology, Faculty of Technology, Tokyo University of Agriculture and Technology, Japan.
Iida
Y
Y
Matsuoka
H
H
Kurata
H
H
D000935:Antifungal Agents / Q000494:pharmacology*; D001234:Aspergillus niger / Q000166:cytology / Q000187:drug effects* / Q000254:growth & development; D015374:Biosensing Techniques; D002455:Cell Division / Q000187:drug effects; D003470:Culture Media; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D008825:Miconazole / Q000378:metabolism; D010946:Plants, Medicinal; D011107:Polylysine / Q000737:chemistry
D000935:Antifungal Agents; D003470:Culture Media; D004365:Drugs, Chinese Herbal; D011107:Polylysine; D008825:Miconazole