Determination of enamel protein synthesized by recombined mouse molar tooth germs in organ culture.
Epithelial-mesenchymal interaction is a prerequisite for tooth morphogenesis. To study this interaction, inner enamel epithelium and dental papilla mesenchyme of molar tooth germs from a 16.5-day mouse embryo were dissociated enzymatically and cultured alone or after recombination. Characteristic matrix protein synthesized and secreted by recombined tooth germ was determined quantitatively by enzyme-linked immunosorbent assay. The protein was detected in the culture of recombined tooth germ but not of dissociated enamel epithelium alone. The amount of enamel protein increased until 8 days in culture. Morphological differentiation of the recombined epithelial rudiment into ameloblasts and enamel protein production were confirmed.
Baba
T
T
Department of Oral Biochemistry, School of Dentistry, Nagasaki University, Japan.
Terashima
T
T
Oida
S
S
Sasaki
S
S
D000565:Ameloblasts; D053523:Amelogenin; D000818:Animals; D002454:Cell Differentiation; D003746:Dental Enamel Proteins / Q000032:analysis / Q000096:biosynthesis*; D004797:Enzyme-Linked Immunosorbent Assay; D004848:Epithelium / Q000502:physiology; D008648:Mesoderm / Q000502:physiology; D051379:Mice; D009805:Odontogenesis; D009924:Organ Culture Techniques; D014083:Tooth Germ / Q000166:cytology* / Q000196:embryology / Q000378:metabolism*
D053523:Amelogenin; C507200:Amelx protein, mouse; D003746:Dental Enamel Proteins