Axonal transport of protein products of the bacterial reporter gene in the brain of transgenic mice.
The aim of the present study was to examine the intracellular localization of ectopically expressed proteins of the bacterial reporter gene in neurons of transgenic mice. In the brain of transgenic mice that carry a chimeric gene composed of the human tyrosine hydroxylase gene promoter and the bacterial gene encoding chloramphenicol acetyltransferase (CAT), the expressed CAT protein was previously found in catecholaminergic neurons as well as in non-catecholaminergic neurons expressing CAT ectopically. In the gene-expressing catecholaminergic neurons, the CAT protein was formerly detected in the axon terminals. In the present study, we immunocytochemically showed that the ectopically expressed protein was localized in cell bodies and axon terminals of the granule cells of the dentate gyrus. This suggests that the ectopically expressed protein of the bacterial gene is transported in the axons to their terminals.
Arai
R
R
Department of Anatomy, Fujita Health University School of Medicine, Japan.
Karasawa
N
N
Nagatsu
I
I
D000818:Animals; D001370:Axonal Transport / Q000235:genetics*; D001426:Bacterial Proteins / Q000378:metabolism*; D001921:Brain / Q000378:metabolism*; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D018891:Dentate Gyrus / Q000378:metabolism; D005798:Genes, Bacterial; D017930:Genes, Reporter; D051379:Mice; D008822:Mice, Transgenic; D014446:Tyrosine 3-Monooxygenase / Q000235:genetics
D001426:Bacterial Proteins; D014446:Tyrosine 3-Monooxygenase; D015500:Chloramphenicol O-Acetyltransferase