title: The effect of fibrin sealant on spinal fusions using allograft in dogs.
abstract: METHODS
  This study investigated the use of Tisseel (immuno [Canada], Toronto, Ontario) as an adjunct to allograft spinal fusion. Thirteen mongrel, dogs were fused bilaterally with morcellized graft from a separate dog.
  
  
  OBJECTIVE
  To evaluate whether fibrin sealant had an effect on bone volume of fusion mass in allograft fusions of the spine.
  
  
  BACKGROUND
  Fibrin sealant has been promoted for use in many orthopedic applications. There is controversy about its effectiveness in augmenting bone graft healing. However, some surgeons make routine use of the sealant in augmentation of bone grafting procedures.
  
  
  METHODS
  To test the usefulness of this material in augmenting allograft fusions, the authors carried out bilateral posterolateral fusions in 13 mongrel dogs. At surgery, 15 cm3 of allograft was placed into a posterolateral position at the L5-L6 region on both sides of the spine. Fibrin sealant (Tisseel) was allocated randomly to one side only. Fusion mass was tested 6 months after the initial operation by computed tomographic scan imaging and mechanical testing.
  
  
  RESULTS
  A significantly smaller bone volume mass, as illustrated by computed tomographic measurement, was seen on the Tisseel side (P = 0.03). Biomechanical testing indicated that there was a trend for the Tisseel side to be stiffer than the untreated side, particularly at lower weights, but statistical significance was not achieved. Computed tomographic volumetric analysis showed that Tisseel-treated allograft led to a significantly smaller fusion volume.
  
  
  CONCLUSIONS
  This study refutes the belief that Tisseel is a good material for accomplishing or augmenting intervertebral arthrodesis. Fibrin sealant significantly retards allograft fusion mass formation in dogs.
authors.affiliation: Santa Cabrini Hospital, Montreal, Quebec, Canada.
authors.forename: P
authors.initials: P
authors.lastname: Jarzem
authors.forename: E J
authors.initials: EJ
authors.lastname: Harvey
authors.forename: R
authors.initials: R
authors.lastname: Shenker
authors.forename: A
authors.initials: A
authors.lastname: Hajipavlou
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena; D016025:Bone Transplantation; D004285:Dogs; D015718:Fibrin Tissue Adhesive / Q000494:pharmacology*; D013123:Spinal Fusion; D014057:Tomography, X-Ray Computed; D014184:Transplantation, Homologous
chemical_list: D015718:Fibrin Tissue Adhesive