Glutamic acid decarboxylase (GAD 67) gene expression in the pancreas and brain of the nonobese diabetic mouse.
A quantitative PCR was developed to measure GAD 67 mRNA levels in pancreas from NOD mice. Two nonautoimmune mouse strains were used as controls. An internal standard, rat GAD 67 mRNA, was prepared to quantify the reaction. In NOD mice GAD 67 expression in the pancreas was in the order of 2 x 10(6) molecules/microgram of total RNA; this expression was five times less in the two control mouse strains, whatever the age. The overexpression of GAD 67 characterized in the NOD mouse could be restricted to the pancreas since similar analysis performed in mouse brain did not reveal a large difference between the three mouse strains studied.
Pleau
J M
JM
CNRS URA 1461, Université Paris V, Hôpital Necker, Paris, France.
Esling
A
A
Van Acker
C
C
Dardenne
M
M
D000375:Aging / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000201:enzymology*; D017931:DNA Primers; D003922:Diabetes Mellitus, Type 1 / Q000201:enzymology* / Q000235:genetics; D005260:Female; D015870:Gene Expression; D005968:Glutamate Decarboxylase / Q000235:genetics*; D007527:Isoenzymes / Q000235:genetics*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D016688:Mice, Inbred NOD; D008969:Molecular Sequence Data; D010179:Pancreas / Q000201:enzymology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats
D017931:DNA Primers; D007527:Isoenzymes; D012333:RNA, Messenger; D005968:Glutamate Decarboxylase