[Effect of Sarcocystis gigantea extract (SGE) on the replication of human immunodeficiency virus (HIV)].
Basis of this study were the previous findings regarding isolation and characterization of a Sarcocystis gigantea lectin (SGL) especially the activation of human mononuclear cells (CD3+, CD4+, CD8+, Mø, B-lymphocytes). HIV-susceptible, immortaliced cell lines (H9-, MT-4) should be investigated to examine their reactivity against SGE which contains this strong mitogen. Using lymphocyte proliferation assay a strong stimulation of noninfected CD(4+)-positive H9-cell by SGE was observed. HIV-infected H(9+)-cells showed after SGE stimulation (20-160 micrograms) an exacerbation with an optimum at day 4. The virus replication in the H(9+)-hostcells was 13 times stronger. This result could also be detected indirectly because of the higher cytotoxicity in the MT-4 cell system. Cytopathogeny was measured by MTT cellvitality assay. In parallel, the high sensitive p24 core Profile-ELISA was used to directly examine the amount of produced HIV. In case of the H(9+)-cells the virus release per cell was 5 times higher after SGE stimulation compared with control.
Drössigk
V U
VU
Institut für Parasitologie und Tropenveterinärmedizin Freien Universität Berlin.
Tietz
H J
HJ
Pötzsch
F
F
Scholz
D
D
Gantenberg
R
R
Hiepe
T
T
D000818:Animals; D002460:Cell Line; D006678:HIV / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D037102:Lectins / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology; D008264:Macrophages / Q000187:drug effects / Q000276:immunology; D012522:Sarcocystis; D014020:Tissue Extracts / Q000494:pharmacology; D014779:Virus Replication / Q000187:drug effects
D037102:Lectins; D014020:Tissue Extracts
Einflüsse von Sarcocystis gigantea-Extrakt (SGE) auf die Replikation des Humanen Immundefiziens Virus (HIV).