[Cytotoxic action of monoclonal antibodies OC125 and SH-9 on ovarian cancer cell lines].
A large number monoclonal antibodies (Mo-Abs) to neoplasms have been utilized as diagnostic tools, but they have not yet been fully characterized from the biological point of view. Antibody-dependent cellular cytotoxicity, complement dependent cytotoxicity (CDC) and antibody-dependent cell-mediated cytotoxicity (ADCC) were investigated in Mo-Abs OC125 and SH-9 (IgG1) to ovarian tumor cell lines SHIN-3, MN-1, KEN-3 and EC by MTT assay in vitro. OC125, but not SH-9 showed prominent CDC activity against SHIN-3 and MN-1 cell lines. CDC activity was induced against OC125 and SH-9, when a guinea pig complement was used. Furthermore, ADCC, activity was significant against SHIN-3, MN-1, OC125 and SH-9, but not against KEN-3 or EC. In summary, OC125 had both CDC and ADCC activity, but SH-9 did not. We concluded that the epitope on CA125 antigen varied in OC125 and SH-9, and each of epitope in Mo-Abs may have a different biofunction.
Murakami
F
F
Department of Obstetrics and Gynecology, Kurume University School of Medicine, Japan.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000920:Antibody-Dependent Cell Cytotoxicity; D018394:CA-125 Antigen / Q000276:immunology; D003165:Complement System Proteins / Q000276:immunology; D003602:Cytotoxicity, Immunologic; D000939:Epitopes / Q000276:immunology; D005260:Female; D006168:Guinea Pigs; D006801:Humans; D010051:Ovarian Neoplasms / Q000276:immunology*; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D018394:CA-125 Antigen; D000939:Epitopes; D003165:Complement System Proteins