Adsorption of bovine serum albumin on to titanium powder.
The adsorption of bovine serum albumin (BSA) on to titanium powder has been studied as a function of protein concentration and pH, and in the presence of calcium and phosphate ions. Isotherm data have shown that the adsorption process does not follow the Langmuir model (inflection points). The maximum adsorption (Admax) at pH 6.8 is 1.13 +/- 0.21 mg m-2. For the pH dependence of adsorption the amount adsorbed increases with decreasing pH (at pH 5.15 Admax = 1.31 +/- 0.2 mg m-2), indicating that hydration effects are important. Adsorption increases and decreases in the presence of calcium (at pH 6.8 for 0.002 M Admax = 1.73 +/- 0.23 mg m-2) and phosphate (at pH 6.8 for 0.002 M Admax = 0.64 +/- 0.14 mgm-2)ions, respectively, indicating that electrostatic effects are important. The time dependence, isotherm and desorption data provide indirect evidence of possible conformational changes in the BSA molecule.
Wassell
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DT
Department of Basic Dental Science, Cardiff Dental School, University of Wales College of Medicine, Cardiff, UK.
Embery
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D000327:Adsorption; D000818:Animals; D001672:Biocompatible Materials; D002118:Calcium; D002130:Calcium Phosphates; D002417:Cattle; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D008422:Materials Testing; D009994:Osmolar Concentration; D010710:Phosphates; D011208:Powders; D012710:Serum Albumin, Bovine; D019346:Sodium Acetate; D013499:Surface Properties; D013997:Time Factors; D014025:Titanium
D001672:Biocompatible Materials; D002130:Calcium Phosphates; D010710:Phosphates; D011208:Powders; C485828:alpha-tricalcium phosphate; C485830:tetracalcium phosphate; D012710:Serum Albumin, Bovine; D019346:Sodium Acetate; C485838:calcium phosphate, monobasic, anhydrous; C020243:calcium phosphate; D014025:Titanium; C485829:calcium phosphate, dibasic, anhydrous; C018279:sodium phosphate; D002118:Calcium