title: Geranyl 6-O-alpha-L-arabinopyranosyl-beta-D-glucopyranoside isolated as an aroma precursor from leaves of a green tea cultivar.
abstract: A new glycosidic aroma precursor was isolated from green tea leaves (Camellia sinensis var. sinensis cv. Yabukita) along with the known primeverosides of cis-linalool 3,6-oxide, linalool and geraniol. These glycosides were separated by chromatographic isolation on Amberlite XAD-2, ODS flash chromatography, and finally HPLC. The chemical structure of the new unknown glycoside was confirmed as geranyl 6-O-alpha-L-arabinopyranosyl-beta-D-glucopyranoside (geranyl beta-vicianoside) by spectrometric analyses and by an enzymatic hydrolysis with glycosidase followed by GC-MS and HPLC analyses. Moreover the vicianoside was hydrolyzed with acetone powder obtained from fresh tea leaves to generate the same compounds, suggesting this glycoside to be a tea aroma precursor.
authors.affiliation: Department of Nutrition and Food Science, Ochanomizu University, Tokyo, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Nishikitani
authors.forename: K
authors.initials: K
authors.lastname: Kubota
authors.forename: A
authors.initials: A
authors.lastname: Kobayashi
authors.forename: F
authors.initials: F
authors.lastname: Sugawara
mesh_terms: D000080462:Acyclic Monoterpenes; D002851:Chromatography, High Pressure Liquid; D005960:Glucosides / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D006868:Hydrolysis; D007306:Insecticides / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D039821:Monoterpenes; D010936:Plant Extracts / Q000737:chemistry* / Q000378:metabolism; D018515:Plant Leaves / Q000737:chemistry; D016339:Spectrometry, Mass, Fast Atom Bombardment; D013662:Tea / Q000737:chemistry*; D013729:Terpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D000080462:Acyclic Monoterpenes; D005960:Glucosides; D007306:Insecticides; D039821:Monoterpenes; D010936:Plant Extracts; D013662:Tea; D013729:Terpenes; C084878:kenposide A; C018584:linalool; C007836:geraniol