title: Cell cycle-dependent localization of tissue inhibitor of metalloproteinases-1 immunoreactivity in cultured human gingival fibroblasts.
abstract: Localization of the tissue inhibitor of metalloproteinases-1 (TIMP-1) was investigated in cultured human gingival fibroblasts by immunohistochemistry. TIMP-1 immunostaining was observed in the cytoplasm of a majority of cells, and in the nucleus of some cells. Depletion of fetal calf serum (FCS) from the culture medium reduced the density of immunoreactive TIMP-1 in the nucleus more remarkably than in the cytoplasm, and the following FCS-induced cell growth was accompanied by a recovery of immuno-reactive TIMP-1 in both the nucleus and the cytoplasm. The frequency of the nuclear TIMP-1-positive cells changed in line with that of PCNA-positive cells and was always much higher than that of S-phase cells, which were estimated by a pulse labelled 5-Bromo-2' deoxyuridine (BrdU). These results suggest a localization of TIMP-1 or a related substance in the nucleus of proliferating human fibroblasts, and its depletion from the nucleus due to an arrest of cell growth.
authors.affiliation: Department of Anatomy, Nagoya University School of Medicine, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Li
authors.forename: K
authors.initials: K
authors.lastname: Nishio
authors.forename: K
authors.initials: K
authors.lastname: Yamashita
authors.forename: T
authors.initials: T
authors.lastname: Hayakawa
authors.forename: T
authors.initials: T
authors.lastname: Hoshino
mesh_terms: D002453:Cell Cycle / Q000502:physiology*; D002460:Cell Line; D005347:Fibroblasts / Q000737:chemistry; D005881:Gingiva / Q000737:chemistry* / Q000166:cytology; D006023:Glycoproteins / Q000032:analysis*; D006801:Humans; D008666:Metalloendopeptidases / Q000037:antagonists & inhibitors*; D011480:Protease Inhibitors / Q000032:analysis*; D019714:Tissue Inhibitor of Metalloproteinases
chemical_list: D006023:Glycoproteins; D011480:Protease Inhibitors; D019714:Tissue Inhibitor of Metalloproteinases; D008666:Metalloendopeptidases