[Beta-heterochromatin in Drosophila: molecular organization and function. Molecular biological analysis of a MAR/SAR DNA sequence in centromere heterochromatin of Drosophila melanogaster].
A molecular biological characteristic of a DNA fragment delta 20p1.4, which is a moderately repetitive sequence of the Drosophila melanogaster genome, is presented. The fragment is present in about 120 copies per haploid genome. The main pool of the delta 20p1.4 homologous DNA can be isolated, along with the nuclear matrix DNA, and consists of Hind III-EcoR I monomers 1.4-1.6 kb in length. The monomers may occur in the genome as both single copies and tandem clusters forming chromosomes fragments up to 6-10 kb in length. The region of the delta 20p1.4 fragment between nucleotides 350 and 905 polymerizes with purified lamin from D. melanogaster. The sites of the fragment that had physical contact with lamin in vitro were determined using an Exo III protection. It was demonstrated that ATATTT, A, and T boxes located in four nonperfect tandem repeats were involved in the contact, both DNA strains reacting with lamin.
Bogachev
S S
SS
Department of Pharmacological Sciences, University Medical Center at Stony Brook, State University of New York at Stony Brook 11794-8651, USA.
Borisevich
I V
IV
Fisher
P A
PA
Shturman
N
N
Sharakhov
I V
IV
D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002467:Cell Nucleus / Q000235:genetics*; D002503:Centromere / Q000235:genetics*; D004247:DNA / Q000235:genetics*; D004331:Drosophila melanogaster / Q000235:genetics*; D016678:Genome; D006570:Heterochromatin / Q000737:chemistry* / Q000502:physiology; D008969:Molecular Sequence Data; D012689:Sequence Homology, Nucleic Acid; D013329:Structure-Activity Relationship
D006570:Heterochromatin; D004247:DNA
Beta-geterokhromatin Drosophila: molekuliarnaia organizatsiia i funktsionirovanie. Molekuliarno-biologicheskiĭ analiz MAR/SAR posledovatel'nosti DNK iz tsentromernogo geterokhromatina Drosophila melanogaster.