title: Cytocompatibility of Ti-6Al-4V and Ti-5Al-2.5Fe alloys according to three surface treatments, using human fibroblasts and osteoblasts.
abstract: Titanium alloys are well known for their superior mechanical properties as well as for their good biocompatibility, making them desirable as surgical implant materials. However, these alloys have been proven to behave poorly in friction since wear particles were often detected in tissues and organs associated with titanium implants. In this paper, three surface treatments were investigated in order to improve the wear resistance and the hardness of Ti-6Al-4V and Ti-5Al-2.5Fe: (a) glow discharge nitrogen implantation (10(17) atoms cm-2), (b) plasma nitriding by plasma diffusion treatment (PDT) and (c) deposition of TiN layer by plasma-assisted chemical vapour deposition (PACVD) additionally to PDT. Surface characterization after the different treatments showed considerable improvement in surface hardness, especially after the two nitriding processes. Moreover, the good corrosion resistance of untreated alloys was maintained. A cell culture model using human cells was chosen to study the effect of such treatments on the cytocompatibility of these materials. The results showed that Ti-5Al-2.5Fe alloy was as cytocompatible as the Ti-6Al-4V alloy and the same surface treatment led to identical biological consequences on both alloys. Nitrogen implantation did not modify at all the cellular behaviour observed on untreated samples. After the two nitriding treatments, cell proliferation and viability appeared to be significantly reduced and the scanning electron microscopy study revealed somewhat irregular surface states. However, osteoblast phenotype expression and protein synthesis capacity were not affected. PDT and PACVD may be interesting alternatives to the physical vapour deposition technique.
authors.affiliation: Laboratoire de Pharmacologie, URA CNRS 1288, Faculté de Médecine de Nancy, France.
authors.forename: K
authors.initials: K
authors.lastname: Bordji
authors.forename: J Y
authors.initials: JY
authors.lastname: Jouzeau
authors.forename: D
authors.initials: D
authors.lastname: Mainard
authors.forename: E
authors.initials: E
authors.lastname: Payan
authors.forename: P
authors.initials: P
authors.lastname: Netter
authors.forename: K T
authors.initials: KT
authors.lastname: Rie
authors.forename: T
authors.initials: T
authors.lastname: Stucky
authors.forename: M
authors.initials: M
authors.lastname: Hage-Ali
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000497:Alloys / Q000009:adverse effects; D000535:Aluminum / Q000009:adverse effects*; D001672:Biocompatible Materials / Q000009:adverse effects; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003094:Collagen / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D005353:Fibronectins / Q000096:biosynthesis; D006801:Humans; D007501:Iron / Q000009:adverse effects*; D007700:Kinetics; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism; D015675:Osteocalcin / Q000378:metabolism; D010641:Phenotype; D019736:Prostheses and Implants; D011506:Proteins / Q000378:metabolism; D013499:Surface Properties; D014025:Titanium / Q000009:adverse effects
chemical_list: D000497:Alloys; D001672:Biocompatible Materials; D005353:Fibronectins; D011506:Proteins; D015675:Osteocalcin; C031462:titanium alloy (TiAl6V4); D003094:Collagen; D000535:Aluminum; D014025:Titanium; D007501:Iron; D000469:Alkaline Phosphatase