Regulation of glucocorticosteroid receptor expression in rat hippocampal cell cultures by nerve growth factor.
Dispersed hippocampal cells cultured in serum-free conditions were used to study the effects of nerve growth factor (NGF) on the expression of type I (mineralocorticosteroid or MR) and type II (glucocorticosteroid or GR) corticosteroid receptors. Cells, plated at a density of 1.2 x 10(6) cells/ml in 60 mm Petri dishes, were mainly identified as neurons (90-95%) and maintained for at least 2 weeks. A 7-day treatment with 10-50 ng NGF/ml induced a concentration-dependent decrease of GR binding (40% decrease) compared to untreated cells. In contrast, MR density was unaffected by a 7-day treatment with 50 ng NGF/ml. Data are discussed as possible direct and/or indirect effects of NGF at the level of both neuronal and glial cells.
Sarrieau
A
A
Douglas Hospital Research Center, Department of Psychiatry, McGill University, Montréal, Québec, Canada.
O'Donnell
D
D
Alonso
R
R
Meaney
M J
MJ
D000818:Animals; D002478:Cells, Cultured; D005260:Female; D006624:Hippocampus / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D009414:Nerve Growth Factors / Q000494:pharmacology*; D009474:Neurons / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D011965:Receptors, Glucocorticoid / Q000096:biosynthesis*; D018161:Receptors, Mineralocorticoid / Q000096:biosynthesis*
D009414:Nerve Growth Factors; D011965:Receptors, Glucocorticoid; D018161:Receptors, Mineralocorticoid