Effect of the type of serum in the medium on sodium ascorbate-induced cytotoxicity.
Sodium ascorbate induced cytotoxicity against human glioblastoma T98G cells in RPMI1640 medium supplemented with fetal bovine serum or human serum samples was studied. Several human serum samples significantly reduced the cytotoxic activity of sodium ascorbate, regardless of sex, age or the disease of the serum donor with or without heat-inactivation of the serum. ESR spectroscopy revealed that this serum effect was not simply due to the alteration of the ascorbyl radical intensity, produced from sodium ascorbate. The present study suggests that the apoptosis-inducing activity of sodium ascorbate might be significantly affected by human serum.
Sakagami
H
H
First Department of Biochemistry, School of Medicine, Showa University, Tokyo, Japan.
Satoh
K
K
Sugaya
K
K
Iida
M
M
Hirota
N
N
Matsumoto
K
K
Kimura
S
S
Gomi
K
K
Taguchi
S
S
Kato
S
S
Takeda
M
M
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000690:Amyotrophic Lateral Sclerosis / Q000097:blood*; D000818:Animals; D001205:Ascorbic Acid / Q000633:toxicity*; D001932:Brain Neoplasms / Q000097:blood*; D002417:Cattle; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003470:Culture Media; D003683:Dehydroascorbic Acid / Q000031:analogs & derivatives / Q000032:analysis; D004578:Electron Spin Resonance Spectroscopy; D005260:Female; D005770:Gastrointestinal Neoplasms / Q000097:blood*; D005909:Glioblastoma; D006801:Humans; D008297:Male; D008875:Middle Aged; D012016:Reference Values; D014407:Tumor Cells, Cultured
D003470:Culture Media; C000820:semidehydroascorbic acid; D001205:Ascorbic Acid; D003683:Dehydroascorbic Acid