Functional analysis of the human guanylin gene promoter.
Guanylin (GCAP-I, guanylate cyclase activating peptide I) and uroguanylin (GCAP-II, guanylate cyclase activating peptide II) are regulatory peptides involved in the regulation of the intestinal chloride / water balance. They share significant structural homology to the E. coli enterotoxin STa, which binds to the particulate guanylyl cyclase C causing diarrhea in mammals. In this study we report the functional analysis of the guanylin / GCAP-I gene promoter region. By means of the luciferase reporter gene assay, we demonstrate a strong promoter activity in T84 cells. Especially the first 160 bp of the 5'-flanking region of the gene seem to be essential for gene induction. Our findings are the basis for further identification of important regulatory elements of the corresponding gene.
Pardigol
A
A
Lower Saxony Institute for Peptide Research, Hannover, Germany.
Mägert
H J
HJ
Hill
O
O
Forssmann
W G
WG
D001483:Base Sequence; D004247:DNA; D005768:Gastrointestinal Hormones; D005786:Gene Expression Regulation; D006801:Humans; D008969:Molecular Sequence Data; D045265:Natriuretic Peptides; D010455:Peptides / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012045:Regulatory Sequences, Nucleic Acid; D017384:Sequence Deletion; D015533:Transcriptional Activation; D014407:Tumor Cells, Cultured
D005768:Gastrointestinal Hormones; D045265:Natriuretic Peptides; D010455:Peptides; C072685:guanylin; D004247:DNA