Site-directed mutagenesis of double-stranded plasmids, domain substitution, and marker rescue by comutagenesis of restriction enzyme sites.
Nickoloff
J A
JA
Department of Cancer Biology, Harvard University School of Public Health, Boston, MA, USA.
Deng
W P
WP
Miller
E M
EM
Ray
F A
FA
D001483:Base Sequence; D004247:DNA / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics / Q000378:metabolism; D004262:DNA Restriction Enzymes / Q000235:genetics*; D005819:Genetic Markers; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D010766:Phosphorylation; D010957:Plasmids / Q000235:genetics*; D016133:Polymerase Chain Reaction; D014162:Transfection / Q000379:methods
D017931:DNA Primers; D005819:Genetic Markers; D004247:DNA; D004262:DNA Restriction Enzymes