Characterization of the cefF gene of Nocardia lactamdurans encoding a 3'-methylcephem hydroxylase different from the 7-cephem hydroxylase.
The cefF gene of Nocardia lactamdurans, encoding a functional 2-oxoglutarate-dependent 3'-methylcephem hydroxylase (deacetoxycephalosporin C hydroxylase) has been found to be closely linked to the pcbC gene in the cephamycin C gene cluster. The open-reading frame is 933 bp long and could encode a protein of M(r) 34,366. Introduction of cefF in the cephamycin-non-producer Streptomyces lividans conferred 3'-methylcephem-hydroxylating activity to the transformants but did not result in hydroxylation at carbon 7 of cephamycin. No 3'-methylcephem hydroxylase activity was observed when the cefF gene was introduced in S. lividans in pIJ699 (a vector containing two transcriptional terminators that prevent read-through expression), which suggests that this gene lacks an independent promoter.
Coque
J J
JJ
Faculty of Biology, University of León, Spain.
Enguita
F J
FJ
Cardoza
R E
RE
Martín
J F
JF
Liras
P
P
D000595:Amino Acid Sequence; D001483:Base Sequence; D002513:Cephamycins / Q000096:biosynthesis*; D003001:Cloning, Molecular; D005798:Genes, Bacterial; D008780:Methyltransferases / Q000235:genetics*; D006899:Mixed Function Oxygenases / Q000235:genetics*; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000235:genetics*; D009615:Nocardia / Q000235:genetics*; D010105:Oxygenases / Q000235:genetics*
D002513:Cephamycins; D009097:Multienzyme Complexes; C093122:7-alpha-cephem-methoxylase; D006899:Mixed Function Oxygenases; D010105:Oxygenases; C045288:deacetoxycephalosporin C hydroxylase; D008780:Methyltransferases