Stability of twenty-five analytes in human serum at 22 degrees C, 4 degrees C, and -20 degrees C.
The stability of 25 analytes from serum of healthy donors was determined at room temperature, 4 degrees C, and -20 degrees C over 48 h, 14 days, and 4 months, respectively. Glucose, blood urea nitrogen, sodium, potassium, chloride, creatinine, calcium, phosphorus, uric acid, total protein, albumin, triglycerides, lipase, total creatine kinase, gamma-glutamyltransferase, iron, magnesium, and cholesterol were stable at all three temperatures for the specified times. Carbon dioxide, aspartate and alanine aminotransferases, lactate dehydrogenase, amylase, alkaline phosphatase, and high-density lipoprotein cholesterol demonstrated some loss over time. Proper storage temperatures and times must be considered for these analytes if measurement is not to take place immediately after specimen collection.
Donnelly
J G
JG
Institute for Biomolecular Studies, Cathologic University of America, Washington, DC, USA.
Soldin
S J
SJ
Nealon
D A
DA
Hicks
J M
JM
D000328:Adult; D001774:Blood Chemical Analysis / Q000379:methods*; D001800:Blood Specimen Collection; D015925:Cryopreservation; D006801:Humans; D008875:Middle Aged; D012016:Reference Values; D013696:Temperature; D014021:Tissue Preservation / Q000379:methods*