Inhibition of mouse hepatitis virus multiplication by phosphorothioate analogues of oligodeoxynucleotides complementary to the leader RNA.
Phosphorothioate oligodeoxynucleotides (PS-oligo) complementary to a leader RNA of mouse hepatitis virus (MHV) were more effective inhibitors of MHV multiplication than natural oligodeoxynucleotides (PO-oligo). Sequence-dependent inhibition of viral multiplication was shown at low concentrations (0.001-0.1 microM) of antisense PS-oligo. Phosphorothioate oligodeoxycytidine, PS-(dC)20 and PS-oligo, which has no significant homology to the MHV sequence, showed inhibitory effects on MHV multiplication at concentrations higher than 0.5 microM. These results showed that PS-oligo was more potent than PO-oligo in inhibition of MHV multiplication and that PS-oligo may inhibit MHV multiplication by two different mechanisms, that is, in sequence-dependent and -independent manners.
Hayashi
M
M
Department of Veterinary Radiology, Rakuno Gakuen University, Ebetsu, Japan.
Maeda
A
A
Watanabe
T
T
Hanaki
K
K
Arai
S
S
Nozaki
S
S
D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D001483:Base Sequence; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D051379:Mice; D008969:Molecular Sequence Data; D006517:Murine hepatitis virus / Q000187:drug effects / Q000502:physiology*; D009838:Oligodeoxyribonucleotides / Q000494:pharmacology; D016376:Oligonucleotides, Antisense / Q000494:pharmacology*; D012333:RNA, Messenger; D013329:Structure-Activity Relationship; D013873:Thionucleotides; D014779:Virus Replication / Q000187:drug effects*
D000998:Antiviral Agents; D009838:Oligodeoxyribonucleotides; D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger; D013873:Thionucleotides