title: Two N-acetyl-D-galactosamine-specific lectins from Phaeolepiota aurea.
abstract: Two lectins, PAL-I and PAL-II, were isolated from the mushroom Phaeolepiota aurea by affinity chromatography on acid-treated Sepharose CL-4B followed by reverse-phase FPLC on ProRPC. Both of the lectins were tetramers of 16 kDa subunits. The lectins had little agglutination activity against native erythrocytes but Pronase treatment of erythrocytes increased the sensitivity to agglutination by the lectins. Both lectins exhibited slight preferences for type A compared with type B and O erythrocytes. In haemagglutination inhibition assays, N-acetylgalactosamine and both anomers of methyl N-acetylgalactosaminide were the best inhibitors.
authors.affiliation: Department of Applied Biological Chemistry, Shizuoka University, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Kawagishi
authors.forename: T
authors.initials: T
authors.lastname: Wasa
authors.forename: T
authors.initials: T
authors.lastname: Murata
authors.forename: T
authors.initials: T
authors.lastname: Usui
authors.forename: A
authors.initials: A
authors.lastname: Kimura
authors.forename: S
authors.initials: S
authors.lastname: Chiba
mesh_terms: D000116:Acetylgalactosamine / Q000378:metabolism*; D001487:Basidiomycota / Q000737:chemistry*; D002240:Carbohydrate Sequence; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D006386:Hemagglutination Tests; D006801:Humans; D037102:Lectins / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology; D008969:Molecular Sequence Data
chemical_list: D037102:Lectins; D000116:Acetylgalactosamine