Surfaces modified with covalently-immobilized adhesive peptides affect fibroblast population motility.
Cell population motility and adhesion of rat skin fibroblasts were evaluated on aminophase glass modified with covalently-immobilized biologically active peptides, specifically, either arginine glycine-aspartic acid-serine (RGDS) or tyrosine-isoleucine-glycine-serine-arginine-glycine (YIGSRG). Fibroblast population motility was decreased and adhesion was increased on substrates modified with covalently immobilized RGDS peptide compared to substrates with the covalently immobilized non-adhesive peptides arginine-glycine-glutamic acid-serine and arginine-aspartic acid-glycine-serine. Fibroblast motility was not significantly changed on substrates modified with covalently-immobilized YIGSRG peptide; however, fibroblast adhesion was decreased on that substrate.
Olbrich
K C
KC
Department of Biomedical Engineering, Rensselaer Polytechnic Institute, Troy, NY 12180-3590, USA.
Andersen
T T
TT
Blumenstock
F A
FA
Bizios
R
R
D000588:Amines / Q000737:chemistry; D000595:Amino Acid Sequence; D000704:Analysis of Variance; D000818:Animals; D001665:Binding Sites; D002448:Cell Adhesion / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D005347:Fibroblasts / Q000166:cytology* / Q000378:metabolism; D005898:Glass / Q000737:chemistry; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D019736:Prostheses and Implants; D051381:Rats; D012710:Serum Albumin, Bovine / Q000737:chemistry; D012867:Skin / Q000166:cytology; D012995:Solubility; D013499:Surface Properties
D000588:Amines; D010455:Peptides; D012710:Serum Albumin, Bovine