title: Isolation and characterization of teichoic acid-lake substance as an adhesin of Staphylococcus aureus to HeLa cells.
abstract: A cell wall component that bound to HeLa cells (HeLa cell-binding CWC) was isolated from a clinical isolate of Staphylococcus aureus. The HeLa cell-binding CWC was resistant to heat (100 C, 1 hr) and proteases, did not stain with Coomassie Brilliant Blue R-250 on SDS-PAGE but stained as a broad band with antiserum against the strain on Western blots. These data suggest that the HeLa cell-binding CWC is not a protein, and may be teichoic acid. Purified teichoic acid bound to HeLa cells, whereas fractions without teichoic acid did not. In Western blots, HeLa cell-binding CWC appeared as a broad band of less than 35 kDa, similar to that of purified teichoic acid. These data suggest that the HeLa cell-binding CWC obtained in this study is teichoic acid. Teichoic acid inhibited S. aureus adherence to HeLa cells and bound to the cells time and dose dependently, in a saturable and reversible manner, and therefore appears to be an adhesin of S. aureus to HeLa cells.
authors.affiliation: Department of Microbiology, Hiroshima University School of Dentistry, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Matsuura
authors.forename: Y
authors.initials: Y
authors.lastname: Miyake
authors.forename: S
authors.initials: S
authors.lastname: Nakashima
authors.forename: H
authors.initials: H
authors.lastname: Komatsuzawa
authors.forename: Y
authors.initials: Y
authors.lastname: Akagawa
authors.forename: H
authors.initials: H
authors.lastname: Suginaka
mesh_terms: D018829:Adhesins, Bacterial / Q000302:isolation & purification*; D001422:Bacterial Adhesion / Q000187:drug effects; D006367:HeLa Cells / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D013211:Staphylococcus aureus / Q000737:chemistry; D013682:Teichoic Acids / Q000378:metabolism / Q000494:pharmacology
chemical_list: D018829:Adhesins, Bacterial; D013682:Teichoic Acids