[Immunodiagnosis of schistosomiasis mansoni with a low parasitic load].
Presently, the schistosomiasis mansoni with low worm burden is frequent, thus immunologic assays of interest for the field diagnosis of Schistosoma mansoni light infections were evaluated here. Assays not assessed before (group I) and those requiring better validation (group II) for the screening of light infections were included in this study. In the group I, the immunofluorescence assays for the detection of IgM antibodies to worm antigens (IgM IFAw) and IgG antibodies to egg antigens (IgG IFAe) gave high levels of sensitivity, specificity, efficiency and predictive value of positive. However, the immunoenzymatic assays for the detection of IgM antibodies to worm antigens (IgM ELISAw) and to egg antigens (IgM ELISAe) had lower levels than the former assays. The assays from the group II designed mostly for the detection of IgG antibodies to same parasite antigens showed good diagnostic performance. The data obtained here contributed to evidenciate at least three category of immunoassays, and we concluded that those from the category I are suitable for seroepidemiologic purposes by keeping their diagnostic features unchanged even varying significantly the intensity of S. mansoni infection.
Lima
D M
DM
Instituto de Medicina Tropical de São Paulo.
Abrantes-Lemos
C P
CP
Hoshino-Shimizu
S
S
Valli
L P
LP
Kanamura
H H
HH
da Silva
L C
LC
Vellosa
S A
SA
D000818:Animals; D000909:Antibodies, Helminth / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000469:parasitology*; D015200:Fluoroimmunoassay; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis*; D007075:Immunoglobulin M / Q000032:analysis*; D010270:Parasite Egg Count; D012550:Schistosoma mansoni / Q000276:immunology*; D012555:Schistosomiasis mansoni / Q000175:diagnosis* / Q000276:immunology / Q000469:parasitology
D000909:Antibodies, Helminth; D007074:Immunoglobulin G; D007075:Immunoglobulin M
Imunodiagnóstico da esquistossomose mansônica com baixa carga parasitária.