Occurrence of lamellar bodies in the rat brain organotypic cultures in normal and pathological conditions.
Lamellar bodies (LB) represent an intracytoplasmic organelles of the cells of different origin and various organs. Their exact function is not known. It has been recently suggested that these organelles may play a role in the protein synthesis and regulation of gene expression. Increased number of LB has been demonstrated in differentiating and growing cells. It suggests their functional relation to enhanced metabolism of the cells. Such conditions for cells establish a tissue culture. We have examined organotypic rat brain cultures maintained under standard conditions, and subsequently exposed to anoxia and neurotoxins: kainic and quinolinic acid. In normal conditions LB were occasionally found in neurons. Their number significantly increased in damaged neurons in the experimental conditions. They have also appeared in some damaged astrocytes. We postulate that LB in the brain are connected with cell degeneration. Their origin from rough endoplasmic reticulum is demonstrated.
Matyja
E
E
Department of Neuropathology, Polish Academy of Sciences, Warszawa.
Renkawek
K
K
D000818:Animals; D001253:Astrocytes / Q000187:drug effects; D001921:Brain / Q000187:drug effects / Q000503:physiopathology* / Q000648:ultrastructure*; D000860:Hypoxia / Q000503:physiopathology*; D066298:In Vitro Techniques; D007608:Kainic Acid / Q000494:pharmacology; D009410:Nerve Degeneration; D015388:Organelles / Q000187:drug effects / Q000648:ultrastructure*; D017378:Quinolinic Acid / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar
D017378:Quinolinic Acid; D007608:Kainic Acid