title: [Methods of saturation analysis in endocrinology].
abstract: The article is a brief review of the development of the method of saturation analysis (MSA) from its beginning to nowdays. Our clinic participated at some stages on this development. Srámková introduced the RIA of GH in 1968, one year later RIA for prolactin and in 1973 RIA for insulin. The extention of the RIA principle to MSA was after its foundation applicated by Justová et al. for the determination of metabolites of vitamin D. Calcitriol, hormon of vitamin D-metabolites was analysed by radioreceptorassay (RRA). The same principle of RRA was applicated at the determination of receptors for insulin by Hilgertová. The application of MSA at our clinic is summarized in table 1. and 2. The home made methods and the choice of commercial kits were verified not only from the point of view of the demands on the quantitative parameters of the method but also from the point of view of the cost of a kit. At our clinic we follow the development of MSA of specific binding proteins (BP), trying to introduce or modify the described techniques f. e. the analyses of BP of GH (GHBP), BP for IGF-I (IGFBP-3, IGFBP-1). The extention of the analysed compounds leads to the precision in the diagnosis and more effective monitoring in the process of treatment. Generally, the article describes the utilization of MSA in laboratories interested not only in the routine work, but in the research activity as well.
authors.affiliation: Laborator pro endokrinologii a metabolismus, 1. lékarské fakulty, Univerzity Karlovy, Praha, Czech Republic.
authors.forename: V
authors.initials: V
authors.lastname: Justová
authors.forename: Z
authors.initials: Z
authors.lastname: Lacinová
mesh_terms: D018153:Czech Republic; D004704:Endocrinology / Q000379:methods; D049673:History, 20th Century; D006801:Humans; D007118:Immunoassay / Q000266:history / Q000379:methods
vernacular_title: Metody saturacní analýzy v endokrinologii.