Detection of Yersinia enterocolitica serogroup O:3 by a PCR method.
Yersinia enterocolitica is the etiologic agent of a range of clinical situations in humans, but only a small number of serotypes are involved. Among these, Y. enterocolitica O:3 is the most frequently implicated. A PCR method was developed to detect Y. enterocolitica O:3. For this purpose, two pairs of primers were designed to amplify two fragments of the rfb cluster of Y. enterocolitica O:3: a 253-bp fragment of the rfbB gene and a 405-bp fragment of the rfbC gene. A specific detection was obtained only with rfbC primers, which yielded a PCR product of the expected size exclusively with pathogenic Y. enterocolitica of serotype O:3. This pair of primers was combined with the ail, inv, and virF primers previously described (H. Nakajima, M. Inoue, T. Mori, K.-I. Itoh, E. Arakawa, and H. Watanabe, J. Clin. Microbiol. 30:2484-2486, 1992) to allow both the detection and the differentiation between Y. pseudotuberculosis, pathogenic Y. enterocolitica of serotype O:3 and other pathogenic Y. enterocolitica.
Weynants
V
V
Unité d'Immunologie-Microbiologie, Facultés Universitaires Notre-Dame de la Paix, Namur, Belgium.
Jadot
V
V
Denoel
P A
PA
Tibor
A
A
Letesson
J J
JJ
D001431:Bacteriological Techniques / Q000706:statistics & numerical data; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D005069:Evaluation Studies as Topic; D005243:Feces / Q000382:microbiology; D005798:Genes, Bacterial; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000706:statistics & numerical data; D012680:Sensitivity and Specificity; D012703:Serotyping; D013045:Species Specificity; D015009:Yersinia Infections / Q000175:diagnosis / Q000382:microbiology; D015008:Yersinia enterocolitica / Q000145:classification* / Q000235:genetics* / Q000472:pathogenicity; D015011:Yersinia pseudotuberculosis / Q000145:classification / Q000235:genetics
D017931:DNA Primers; D004269:DNA, Bacterial