Sequence and characterization of a novel chromosomal aminoglycoside phosphotransferase gene, aph (3')-IIb, in Pseudomonas aeruginosa.
A novel, probably chromosomally encoded, aminoglycoside phosphotransferase gene was cloned on a 2,996-bp PstI fragment from Pseudomonas aeruginosa and designated aph (3')-IIb. It coded for a protein of 268 amino acids that showed 51.7% amino acid identity with APH (3')-II [APH(3') is aminoglycoside-3' phosphotransferase] from Tn5. Two other open reading frames on the cloned fragment showed homology to a signal-transducing system in P. aeruginosa.
Hächler
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Institute of Medical Microbiology, University of Zürich, Switzerland. haechler@immv.unizh.ch
Santanam
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Kayser
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FH
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000032:analysis / Q000302:isolation & purification; D004352:Drug Resistance, Microbial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial / Q000235:genetics*; D019868:Kanamycin Kinase; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics*; D010957:Plasmids / Q000032:analysis; D011550:Pseudomonas aeruginosa / Q000201:enzymology / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D015398:Signal Transduction; D014161:Transduction, Genetic
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D017853:Phosphotransferases (Alcohol Group Acceptor); D019868:Kanamycin Kinase