Toxicity and properties of the extract from Sarcocystis cruzi cysts.
The extract from Sarcocystis cruzi cysts in bovine muscle was subcutaneously injected to mice, guinea pigs, chickens, and rabbits to detect its toxicity. Only rabbits showed reactions after administration of the extract at a dose of 25 micrograms. The main clinical signs of the rabbits were depression, reduction in body temperature and intermittent diarrhea and the hematological findings observed were elevation in WBC, RBC, PCV, TP, BUN, AST, AUT and creatinine values and reduction in glucose, K+ and pH of blood. The extract, crude toxin, was a water soluble, acid-alkali stable and thermolabile protein and estimated to be a molecular mass of 15-16 kd.
Saito
M
M
Kumagaya Meat Inspection Center Saitama Prefecture, Japan.
Taguchi
K
K
Shibata
Y
Y
Kobayashi
T
T
Shimura
K
K
Itagaki
H
H
D000818:Animals; D001685:Biological Factors / Q000302:isolation & purification / Q000633:toxicity*; D001786:Blood Glucose / Q000378:metabolism; D001831:Body Temperature / Q000187:drug effects; D002417:Cattle; D002418:Cattle Diseases; D002645:Chickens; D003643:Death; D003967:Diarrhea; D004305:Dose-Response Relationship, Drug; D004906:Erythrocyte Count; D006168:Guinea Pigs; D007958:Leukocyte Count; D051379:Mice; D018482:Muscle, Skeletal / Q000469:parasitology*; D009928:Organ Specificity; D011817:Rabbits; D012522:Sarcocystis / Q000302:isolation & purification; D012523:Sarcocystosis / Q000473:pathology / Q000662:veterinary*
D001685:Biological Factors; D001786:Blood Glucose