[Simplified method of cultured brain microcapillary endothelial cells].
Many kinds of methods cultured brain microcapillary endothelial cells have been published, but these methods were not established. We designed the simplified methods with improvement of enzymatic treatment and Percoll gradient centrifugation.
Suyama
T
T
Department of Neurosurgery, Kansai Medical University, Osaka, Japan.
Tsuchida
T
T
Matsumura
K
K
Sakai
N
N
Kawamoto
K
K
Kishimoto
T
T
Tada
M
M
D000818:Animals; D001921:Brain / Q000098:blood supply*; D002196:Capillaries / Q000166:cytology; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D003584:Cytological Techniques; D004730:Endothelium, Vascular / Q000166:cytology*; D013552:Swine