[Effects of alpha 1-adrenergic receptor subtypes activation on proliferation of smooth muscle cells in rabbit aorta].
The effect of alpha 1-Adrenoceptor (alpha 1-AR) and its subtypes, alpha 1A and alpha 1B, activation on the proliferation of cultured vascular smooth muscle cells (VSMC) was studied by [3H]-thymidine (3H-TdR) incorporation and in situ hybridization. Norepinephrine (NE) promotes DNA synthesis of VSMC. The action was induced by activation of alpha 1A-AR subtype and increase of c-fos gene expression. However, the DNA synthesis was inhibited by activation of alpha 1B-AR subtype. There was a synergetic effect on VSMC DNA synthesis between NE and neuropeptide Y (NPY). But only additional effect was found between NE and Ang II. More over, it was found that alpha 1B-AR subtype showed significant constitutive inhibitory effect of VSMC DNA synthesis.
Li
Y
Y
Institute of Vascular Medicine, Third School of Clinical Medicine, Beijing Medical University.
Han
C D
CD
D000818:Animals; D001013:Aorta, Thoracic / Q000166:cytology; D002455:Cell Division; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis*; D005260:Female; D015870:Gene Expression; D016762:Genes, fos; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000166:cytology*; D011817:Rabbits; D018340:Receptors, Adrenergic, alpha-1 / Q000145:classification / Q000502:physiology*; D018342:Receptors, Adrenergic, beta-1 / Q000502:physiology*
D018340:Receptors, Adrenergic, alpha-1; D018342:Receptors, Adrenergic, beta-1; D004247:DNA