Interleukin-1 beta stimulates glucose uptake of human peritoneal mesothelial cells in vitro.
To investigate whether the glucose uptake (GU) of human peritoneal mesothelial cells (HPMC) is mediated by glucose transporters and whether this uptake is influenced by interleukin 1-beta (IL-1 beta), we measured 2-deoxy-(3H)-GU of HPMC in vitro, after exposing the cells for different times (two and 12 hours) to increasing concentrations (0.1, 1.0, and 2.0 ng/mL) of IL-1 beta. To exclude a noncarrier-mediated transport, GU was also tested in the presence of cytochalasin B. All experiments were performed in triplicate in the cells of two donors. Cytochalasin B inhibits GU of HPMC almost completely. GU of HPMC is not stimulated by insulin. GU is stimulated by IL-1 beta in a dose-dependent manner. These data indicate a GU of HPMC, which is mediated by a glucose transporter and stimulated by IL-1 beta. The increased uptake of glucose from the dialysate in patients with peritonitis may be mediated by a (cytokine-induced) increased activity of HPMC glucose transporters.
Kruse
M
M
Department of Nephrology, Hannover Medical School, Germany.
Mahiout
A
A
Kliem
V
V
Kurz
P
P
Koch
K M
KM
Brunkhorst
R
R
D001786:Blood Glucose / Q000378:metabolism*; D003571:Cytochalasin B / Q000494:pharmacology; D003847:Deoxyglucose / Q000378:metabolism; D015314:Dialysis Solutions / Q000493:pharmacokinetics; D004305:Dose-Response Relationship, Drug; D004847:Epithelial Cells; D006801:Humans; D066298:In Vitro Techniques; D007375:Interleukin-1 / Q000494:pharmacology*; D009004:Monosaccharide Transport Proteins / Q000502:physiology; D010537:Peritoneum / Q000166:cytology*; D010538:Peritonitis / Q000473:pathology
D001786:Blood Glucose; D015314:Dialysis Solutions; D007375:Interleukin-1; D009004:Monosaccharide Transport Proteins; D003571:Cytochalasin B; D003847:Deoxyglucose