Role of tissue inhibitors of metalloproteinase in diabetic nephropathy.
We evaluated the role of tissue inhibitors of metalloproteinase-1 (TIMP-1) in patients with diabetic nephropathy by comparing the serum and urine TIMP-1 levels with those of renal biopsy specimens. A total of 35 diabetic patients were divided into four groups, D0, DI, DII and DIII-IV, according to the severity of diffuse glomerular lesions using Gellman's criteria. Using serum and 24-hour urine specimens, TIMP-1 was measured by a sandwich enzyme immunoassay. Serum and urinary TIMP-1 showed significant increases in association with the progress of glomerular diffuse lesions. There was no correlation between serum TIMP-1 and serum creatinine, creatinine clearance, serum and urinary beta 2-microglobulin, urinary NAG, HbA1c, or urinary TIMP-1. There was a significant correlation between urinary TIMP-1 and urinary albumin, and was a significant correlation between urinary TIMP-1 and urinary NAG. We conclude that TIMP-1 has a potential role in the regulation of glomerular matrix accumulation in diabetic nephropathy.
Kanauchi
M
M
First Department of Internal Medicine, Nara Medical University, Japan.
Nishioka
H
H
Nakashima
Y
Y
Hashimoto
T
T
Dohi
K
K
D000328:Adult; D000368:Aged; D003928:Diabetic Nephropathies / Q000209:etiology* / Q000378:metabolism; D005109:Extracellular Matrix / Q000378:metabolism; D005260:Female; D006023:Glycoproteins / Q000378:metabolism / Q000502:physiology*; D006801:Humans; D007678:Kidney Glomerulus / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D011480:Protease Inhibitors / Q000378:metabolism; D012720:Severity of Illness Index; D019714:Tissue Inhibitor of Metalloproteinases
D006023:Glycoproteins; D011480:Protease Inhibitors; D019714:Tissue Inhibitor of Metalloproteinases