[Purification of serotype d-- and e--specific antigens from Actinobacillus actinomycetemcomitans and seroclassification of clinical isolates from periodontal patients].
Oral Actinobacillus actinomycetemcomitans strains have been classified into five serotypes. The aim of this study was to determine the compositions of A. actinomycetemcomitans serotype d- and e- specific antigens. The serodistribution of clinical isolates from the patients with periodontitis were also investigated. Serotype-specific polysaccharide antigens of A. actinomycetemcomitans IDH 781 (serotype d) and OMZ 534 (serotype e) were extracted from whole cells by autoclaving. The extracts were purified by chromatography on DEAE-Sepharose CL-6B and Sephacryl S-300HR columns. The serotype d antigen was composed of rhamnose (17.1%), mannose (45.5%), galactose (2.0%) and glucose (35.5%). On the other hand, the serotype e antigen was composed of rhamnose (23.9%), mannose (29.1%), galactose (11.0%), glucose (13.5%) and unidentified sugar (22.5%). Immunodiffusion tests revealed that the purified polysaccharide antigen form a single precipitin line with the corresponding rabbit antiserum. A total of 157 A. actinomycetemcomitans clinical isolates from diseased sites of 39 patients with periodontitis were serotyped by using serotype-specific rabbit antisera against A. actinomycetemcomitans serotype a, b, c, d and e strains. In the immunodiffusion assay, the autoclaved extracts of 42, 12, 34, 8 and 41 A. actinomycetemcomitans clinical isolates reacted with serotype a, b, c, d and e antisera, respectively. These findings indicate that the extraction of serotype antigens by autoclaving is useful and definite for the serotyping of A. actinomycetemcomitans clinical isolates.
Yamamoto
M
M
Department of Periodontology, Tokyo Medical and Dental University, Japan.
D000293:Adolescent; D000328:Adult; D016976:Aggregatibacter actinomycetemcomitans / Q000145:classification* / Q000276:immunology; D000942:Antigens, Bacterial / Q000302:isolation & purification*; D002648:Child; D000939:Epitopes / Q000302:isolation & purification*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010518:Periodontitis / Q000382:microbiology*; D011135:Polysaccharides, Bacterial / Q000302:isolation & purification*; D012703:Serotyping
D000942:Antigens, Bacterial; D000939:Epitopes; D011135:Polysaccharides, Bacterial