Plasminogen activator inhibitor type-2 in the lesional epidermis of lupus erythematosus.
Under certain pathophysiological conditions epidermal keratinocytes produce urokinase-type plasminogen activator (uPA) or tissue-type PA (tPA). These PAs are subject to regulation by PA inhibitors (PAI), including PAI type-2 (PAI-2). In the normal epidermis, PAI-2 is present in the differentiating suprabasal layers, albeit in the apparent absence of PAs. It has, therefore, been suggested that PAI-2 plays a role in epidermal differentiation not linked to its ability to inhibit PAs. In line with this hypothesis, we have studied, by immunohistochemistry, the distribution of PAI-2, uPA and tPA in the normal and in the lesional epidermis of patients with lupus erythematosus (LE), a disease in which epidermal differentiation is disturbed. The PAI-2 antigen was detectable in the normal epidermis and in the lesional epidermis of LE. In the normal epidermis, the PAI-2 antigen was most pronounced in the granular layer. In the hyperkeratotic epidermal lesions of LE, the PAI-2 antigen was increased. In normal and lesional skin, PAI-2 was distributed along the cell periphery, indicating its association with the cornified envelope. Neither uPA nor tPA was detectable in normal or lesional epidermis. Our findings show that PAI-2 is a major type of PAI in normal epidermis and in the lesional epidermis of LE, and that increased epidermal PAI-2 is observed in a disease which is not associated with an increase in epidermal PAs. The data support the hypothesis that epidermal PAI-2 may have other functions than the regulation of PA activity.
Bechtel
M J
MJ
University Institute for Immunology, Laboratory for Immunopathology, Heidelberg, Germany.
Schaefer
B M
BM
Kramer
M D
MD
D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000201:enzymology; D017396:Plasminogen Activator Inhibitor 2 / Q000032:analysis*; D011498:Protein Precursors / Q000032:analysis; D012867:Skin / Q000737:chemistry / Q000166:cytology / Q000201:enzymology; D010959:Tissue Plasminogen Activator / Q000032:analysis; D011503:Transglutaminases / Q000032:analysis; D014568:Urokinase-Type Plasminogen Activator / Q000032:analysis
D017396:Plasminogen Activator Inhibitor 2; D011498:Protein Precursors; C032253:involucrin; D011503:Transglutaminases; D010959:Tissue Plasminogen Activator; D014568:Urokinase-Type Plasminogen Activator