title: Cytokine profiles of Porphyromonas gingivalis-reactive T lymphocyte line and clones derived from P. gingivalis-infected subjects.
abstract: Porphyromonas gingivalis is generally recognized as a major periodontopathogen such that a study of T cell responses to this organism may help to elucidate immune regulation in periodontal disease.
  
  
  OBJECTIVE
  The aim of this study was to examine interleukin (IL)-4, interferon (IFN)-gamma and IL-10 production by P. gingivalis-responsive T cell lines and clones derived from the peripheral blood of two P. gingivalis-infected subjects with different disease expression and from the gingival tissues of one of the P. gingivalis-infected subjects.
  
  
  METHODS
  FACS analysis was used to determine the percentage of T cells staining positive for cytoplasmic IL-4, IFN-gamma and IL-10 and reverse transcriptase polymerase chain reaction (RT-PCR) was performed to determine the presence of mRNA for IL-4 and IFN-gamma in the T cell lines and clones.
  
  
  RESULTS
  FACS analysis showed that virtually all the T cell lines and clones contained IL-4- and IFN-gamma-producing T cells. The RT-PCR results generally supported this trend. However, a higher percentage of cells in the clones derived from one subject produced IL-4 while a lower percentage produced IFN-gamma compared with the clones derived from the other subject. FACS analysis also demonstrated that the lines and clones derived from the two subjects showed differences in IL-10 production.
  
  
  CONCLUSIONS
  This study has demonstrated that there may be differences in IL-4 and IL-10 production by the P. gingivalis responsive lines and clones derived from P. gingivalis-infected subjects with different disease expression. Any relationship to disease however, remains to be determined.
authors.affiliation: Department of Dentistry, The University of Queensland, Brisbane, Australia.
authors.forename: E
authors.initials: E
authors.lastname: Gemmell
authors.forename: M
authors.initials: M
authors.lastname: Kjeldsen
authors.forename: K
authors.initials: K
authors.lastname: Yamazaki
authors.lastname: Nakajima
authors.forename: M J
authors.initials: MJ
authors.lastname: Aldred
authors.lastname: Seymour
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D000911:Antibodies, Monoclonal; D001483:Base Sequence; D002999:Clone Cells; D016207:Cytokines / Q000096:biosynthesis* / Q000235:genetics; D017931:DNA Primers; D003773:Dental Plaque / Q000382:microbiology; D018450:Disease Progression; D004285:Dogs; D005434:Flow Cytometry; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis / Q000235:genetics; D016753:Interleukin-10 / Q000096:biosynthesis / Q000235:genetics; D007376:Interleukin-2 / Q000096:biosynthesis / Q000235:genetics; D015847:Interleukin-4 / Q000096:biosynthesis / Q000235:genetics; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D010510:Periodontal Diseases / Q000276:immunology* / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods; D016966:Porphyromonas gingivalis / Q000276:immunology*; D012329:RNA, Bacterial / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D016207:Cytokines; D017931:DNA Primers; D007376:Interleukin-2; D012329:RNA, Bacterial; D012333:RNA, Messenger; D016753:Interleukin-10; D015847:Interleukin-4; D007371:Interferon-gamma