Nitric oxide induces cultured cortical neuron apoptosis.
A series of experiments were designed to examine the potential induction of apoptosis by nitric oxide (NO) donors on cortical neuronal cell culture. A 24 h exposure of three different NO donors, 3-morpholinosydnonimine, S-nitroso-N-acetyl-penicillamine, and S-nitrosoglutathione, induced apoptosis as indicated by following histological (cellular and nuclear morphology) and biochemical markers (DNA oligonucleosomal fragmentation and protection by protein synthesis inhibitor).
Palluy
O
O
URA-CNRS 1485, Faculté de Médecine et de Pharmacie de Limoges, France.
Rigaud
M
M
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology* / Q000187:drug effects; D004247:DNA / Q000096:biosynthesis; D004587:Electrophoresis, Agar Gel; D005978:Glutathione / Q000031:analogs & derivatives / Q000494:pharmacology; D008981:Molsidomine / Q000031:analogs & derivatives / Q000494:pharmacology; D009419:Nerve Tissue Proteins / Q000096:biosynthesis; D009474:Neurons / Q000187:drug effects*; D009569:Nitric Oxide / Q000494:pharmacology*; D009603:Nitroso Compounds / Q000494:pharmacology; D010396:Penicillamine / Q000031:analogs & derivatives / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D026423:S-Nitroso-N-Acetylpenicillamine; D026422:S-Nitrosoglutathione
D009419:Nerve Tissue Proteins; D009603:Nitroso Compounds; D009569:Nitric Oxide; D026422:S-Nitrosoglutathione; C002385:linsidomine; D026423:S-Nitroso-N-Acetylpenicillamine; D004247:DNA; D008981:Molsidomine; D005978:Glutathione; D010396:Penicillamine