Metabolic fluxes in chemostat cultures of Schizosaccharomyces pombe grown on mixtures of glucose and ethanol.
Simultaneous utilization of glucose and ethanol by the yeast Schizosaccharomyces pombe CBS 356 was studied in aerobic chemostat cultures. In glucose-limited cultures, respirofermentative metabolism occurred at growth rates above 0.16 h-1. Although Sch. pombe lacks a functional glyoxylate cycle and therefore cannot utilize ethanol as a sole carbon source, ethanol was co-consumed by glucose-limited chemostat cultures. As a result, biomass yields increased, but not up to the theoretical value [0.92 g biomass (g glucose)-1] expected if all of the acetyl-CoA produced from glucose was instead synthesized from ethanol. When ethanol accounted for more than 30% of the substrate carbon in the mixed feed, it was incompletely utilized. In mixed-substrate cultures with a saturating ethanol fraction in the feed, the increase of the biomass yield as a result of ethanol consumption was highest at low dilution rates. This was not due to an increased specific rate of ethanol consumption at low growth rates; rather, the longer residence times at low dilution rates allowed Sch. pombe to utilize a larger fraction of the available ethanol, part of which was oxidized to acetate. Activities of gluconeogenic and glyoxylate-cycle enzymes were not detected in cell-free extracts of any of the cultures. Activities of acetaldehyde dehydrogenase and acetyl-CoA synthetase were low and of the same order of magnitude as the in vivo rates of acetate activation to acetyl-CoA. The results show that ethanol is a poor substrate for Sch. pombe, even as an auxiliary energy source.
de Jong-Gubbels
Patricia
P
Department of Microbiology and Enzymology, Kluyver Laboratory of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC Delft, The Netherlands.
van Dijken
Johannes P
JP
Department of Microbiology and Enzymology, Kluyver Laboratory of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC Delft, The Netherlands.
Pronk
Jack T
JT
Department of Microbiology and Enzymology, Kluyver Laboratory of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC Delft, The Netherlands.
D000255:Adenosine Triphosphate / Q000378:metabolism; D000332:Aerobiosis; D018533:Biomass; D004734:Energy Metabolism; D004798:Enzymes / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism / Q000494:pharmacology*; D005285:Fermentation; D005656:Fungal Proteins / Q000378:metabolism; D005947:Glucose / Q000378:metabolism / Q000494:pharmacology*; D012568:Schizosaccharomyces / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*
D004798:Enzymes; D005656:Fungal Proteins; D000431:Ethanol; D000255:Adenosine Triphosphate; D005947:Glucose