Characterization of clinical isolates of Streptococcus agalactiae by random amplified polymorphic DNA using degenerate oligonucleotides.
Epidemiological studies of Streptococcus agalactiae strains have been limited by the lack of sensitive and discriminatory methods for comparing clinical isolates. Serotyping, albeit a widely used methodology, has been shown to possess low capability to distinguish between epidemiologically related and unrelated isolates. We have employed here a random amplification of polymorphic DNA (RAPD) assay, using degenerate oligonucleotides as primers, to characterize S. agalactiae isolates from related or unrelated clinical samples. Epidemiologically-related isolates (mother-infant pairs) showed identical profiles by this methodology. On the contrary, 12 epidemiologically-unrelated isolates (classified into 5 different serotypes) resulted in 11 distinct RAPD patterns. This suggests that the proposed modified RAPD assay provides a highly discriminatory tool for the analysis of genomic diversity among isolates from pathogenic organisms.
Limansky
A S
AS
Departamento de Microbiología, Universidad Nacional de Rosario.
Guardati
M C
MC
Sutich
E G
EG
Toresani
I E
IE
Bogado
I E
IE
Joannas
G M
GM
Viale
A M
AM
D017931:DNA Primers; D005260:Female; D016680:Genome, Bacterial; D006801:Humans; D007231:Infant, Newborn; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D019105:Random Amplified Polymorphic DNA Technique; D012703:Serotyping; D013292:Streptococcus agalactiae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*
D017931:DNA Primers