IGF-I potentiates interleukin-2 production in human peripheral T cells.
IGF-I stimulates the proliferation and differentiation of many cell types. In the case of T cells, IGF-I has been described to potentiate mitogen-induced DNA synthesis. We have addressed the working mechanism of IGF-I on T cell proliferation by measuring the effects of IGF-I on various stages of T cell activation. We found that IGF-I augmented the phytohaemagglutinin- and anti-CD3-induced interleukin-2 (IL2) production of human peripheral T cells before they enter the S phase of the cell cycle. Furthermore, the addition of IGF-I did not influence DNA synthesis of IL2-dependent growing T cells.
Kooijman
R
R
Department of Immunology, University Hospital for Children, Utrecht, Netherlands.
Rijkers
G T
GT
Zegers
B J
BJ
D000328:Adult; D017252:CD3 Complex; D018929:Cell Culture Techniques; D002455:Cell Division / Q000187:drug effects; D004247:DNA / Q000096:biosynthesis; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D007376:Interleukin-2 / Q000096:biosynthesis* / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects*; D010835:Phytohemagglutinins / Q000494:pharmacology; D016192:Resting Phase, Cell Cycle; D016196:S Phase; D013601:T-Lymphocytes / Q000166:cytology / Q000378:metabolism*
D017252:CD3 Complex; D007376:Interleukin-2; D010835:Phytohemagglutinins; D007334:Insulin-Like Growth Factor I; D004247:DNA