Hypoxic-ischemic injury acutely disrupts microtubule-associated protein 2 immunostaining in neonatal rat brain.
We evaluated the influence of an acute hypoxic-ischemic insult on the neuronal dendritic cytoskeletal protein microtubule-associated protein 2 (MAP2) in the immature rat brain. Studies were performed using a well-characterized perinatal rodent stroke model, in which unilateral ischemic forebrain injury was induced in 7-day-old rats by right carotid artery ligation, followed by 3 h exposure to 8% oxygen. Changes in the neuroanatomic distribution of MAP2 in the first 48 h after injury were evaluated by immunocytochemistry with a monoclonal mouse anti-MAP2 antibody. The distribution of MAP2 immunoreactivity was markedly disrupted in the lesioned hippocampus; prominent reductions in MAP2 immunostaining evolved concurrently in lesioned cortex, caudate and thalamus. These data demonstrate that at this developmental stage, MAP2 immunocytochemistry provides a sensitive indicator of the distribution and severity of hypoxic-ischemic neuronal injury.
Malinak
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Department of Pediatrics, University of Michigan, Ann Arbor 48109-0646, USA.
Silverstein
F S
FS
D000818:Animals; D000831:Animals, Newborn; D000911:Antibodies, Monoclonal; D001921:Brain / Q000378:metabolism*; D001923:Brain Chemistry; D002545:Brain Ischemia / Q000378:metabolism*; D002540:Cerebral Cortex / Q000737:chemistry; D006624:Hippocampus / Q000737:chemistry; D002534:Hypoxia, Brain / Q000378:metabolism*; D007150:Immunohistochemistry; D037102:Lectins / Q000378:metabolism; D051379:Mice; D008869:Microtubule-Associated Proteins / Q000032:analysis / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013788:Thalamus / Q000737:chemistry; D014018:Tissue Distribution
D000911:Antibodies, Monoclonal; D037102:Lectins; D008869:Microtubule-Associated Proteins