Isolation and characterization of teichoic acid-lake substance as an adhesin of Staphylococcus aureus to HeLa cells.
A cell wall component that bound to HeLa cells (HeLa cell-binding CWC) was isolated from a clinical isolate of Staphylococcus aureus. The HeLa cell-binding CWC was resistant to heat (100 C, 1 hr) and proteases, did not stain with Coomassie Brilliant Blue R-250 on SDS-PAGE but stained as a broad band with antiserum against the strain on Western blots. These data suggest that the HeLa cell-binding CWC is not a protein, and may be teichoic acid. Purified teichoic acid bound to HeLa cells, whereas fractions without teichoic acid did not. In Western blots, HeLa cell-binding CWC appeared as a broad band of less than 35 kDa, similar to that of purified teichoic acid. These data suggest that the HeLa cell-binding CWC obtained in this study is teichoic acid. Teichoic acid inhibited S. aureus adherence to HeLa cells and bound to the cells time and dose dependently, in a saturable and reversible manner, and therefore appears to be an adhesin of S. aureus to HeLa cells.
Matsuura
T
T
Department of Microbiology, Hiroshima University School of Dentistry, Japan.
Miyake
Y
Y
Nakashima
S
S
Komatsuzawa
H
H
Akagawa
Y
Y
Suginaka
H
H
D018829:Adhesins, Bacterial / Q000302:isolation & purification*; D001422:Bacterial Adhesion / Q000187:drug effects; D006367:HeLa Cells / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D013211:Staphylococcus aureus / Q000737:chemistry; D013682:Teichoic Acids / Q000378:metabolism / Q000494:pharmacology
D018829:Adhesins, Bacterial; D013682:Teichoic Acids