title: A hyperphosphorylated form of the large subunit of RNA polymerase II is associated with splicing complexes and the nuclear matrix.
abstract: A hyperphosphorylated form of the largest subunit of RNA polymerase II (pol IIo) is associated with the pre-mRNA splicing process. Pol IIo was detected in association with a subset of small nuclear ribonucleoprotein particle and Ser-Arg protein splicing factors and also with pre-mRNA splicing complexes assembled in vitro. A subpopulation of pol IIo was localized to nuclear "speckle" domains enriched in splicing factors, indicating that it may also be associated with RNA processing in vivo. Moreover, pol IIo was retained in a similar pattern following in situ extraction of cells and was quantitatively recovered in the nuclear matrix fraction. The results implicate nuclear matrix-associated hyperphosphorylated pol IIo as a possible link in the coordination of transcription and splicing processes.
authors.affiliation: Department of Biological Sciences, State University of New York at Buffalo 14260, USA.
authors.forename: M J
authors.initials: MJ
authors.lastname: Mortillaro
authors.forename: B J
authors.initials: BJ
authors.lastname: Blencowe
authors.forename: X
authors.initials: X
authors.lastname: Wei
authors.forename: H
authors.initials: H
authors.lastname: Nakayasu
authors.forename: L
authors.initials: L
authors.lastname: Du
authors.forename: S L
authors.initials: SL
authors.lastname: Warren
authors.forename: P A
authors.initials: PA
authors.lastname: Sharp
authors.forename: R
authors.initials: R
authors.lastname: Berezney
mesh_terms: D000818:Animals; D004173:Dipodomys; D018604:Epitope Mapping; D019084:Fluorescent Antibody Technique, Indirect; D006367:HeLa Cells; D006801:Humans; D046911:Macromolecular Substances; D008297:Male; D015530:Nuclear Matrix / Q000201:enzymology*; D010750:Phosphoproteins / Q000276:immunology; D010766:Phosphorylation; D012319:RNA Polymerase II / Q000276:immunology / Q000378:metabolism*; D012326:RNA Splicing; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D046911:Macromolecular Substances; D010750:Phosphoproteins; D012319:RNA Polymerase II