Surface display on staphylococci: a comparative study.
Two different host-vector expression systems, designed for cell surface display of heterologous receptors on Staphylococcus xylosus and Staphylococcus carnosus, respectively, were compared for the surface display of four variants of a 101 amino acid region derived from the G glycoprotein of human respiratory syncytial virus (RSV). Surface localization of the different chimeric receptors was evaluated by a colorimetric assay and by fluorescence-activated cell sorting. It was concluded that the S. carnosus system was better both in the ability to translocate inefficiently secreted peptides and in the number of exposed hybrid receptors. The potential use of the described staphylococci as live bacterial vaccine vehicles or alternatives to filamentous phages for surface display of protein libraries is discussed.
Robert
A
A
Centre d'Immunologie Pierre Fabre, Saint-Julien en Genevois, France.
Samuelson
P
P
Andréoni
C
C
Bächi
T
T
Uhlén
M
M
Binz
H
H
Nguyen
T N
TN
Ståhl
S
S
D005434:Flow Cytometry; D005453:Fluorescence; D005822:Genetic Vectors; D015707:HN Protein; D006801:Humans; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D011993:Recombinant Fusion Proteins / Q000032:analysis; D012136:Respiratory Syncytial Viruses; D013210:Staphylococcus / Q000235:genetics*; D014170:Transformation, Genetic; D014759:Viral Envelope Proteins / Q000032:analysis / Q000235:genetics*; D014764:Viral Proteins / Q000032:analysis / Q000235:genetics*
D015707:HN Protein; D011993:Recombinant Fusion Proteins; D014759:Viral Envelope Proteins; D014764:Viral Proteins; C116204:attachment protein G