title: Characterization of monoclonal antibodies against infections laryngotracheitis virus.
abstract: Nine monoclonal antibodies (MCAs) produced against two different strains of infectious laryngotracheitis virus (ILTV) were characterized and compared to previously characterized MCA 131-6, produced against a third ILTV strain. In western blotting experiments, MCAs C, E, and 11 resembled MCA 131-6, detecting proteins of 205, 160, 115, and 90 kD as well as several proteins less than 49 kD. The other six MCAs differed from previously described ILTV MCA. MCA D detected a 90-kD protein along with several less than 49 kD. MCAs 4 and 5 each detected proteins of 205, 160, 100, 90, and 70 kD. MCA 9 detected the same proteins detected by MCAs 4 and 5 except the 160-kD protein. MCA 10 detected proteins of 100, 90, and 70 kD and several proteins less than 49 kD. MCAs C, D, and E, like MCA 131-6, failed to react with any ILTV grown in the presence of tunicamycin, suggesting that those MCAs are specific for carbohydrate-based epitopes. MCA 6 reacted with only a 100-kD protein in the presence or absence of tunicamycin. The remaining MCA detected only a 70-kD protein in the presence of tunicamycin except MCA 5, which reacted with proteins of 70 and 90 kD. Only MCA 4 and 6 neutralized ILTV infectivity.
authors.affiliation: College of Veterinary Medicine, Oregon State University, Corvallis 97331, USA.
authors.forename: F
authors.initials: F
authors.lastname: Abbas
authors.forename: J R
authors.initials: JR
authors.lastname: Andreasen
authors.forename: R J
authors.initials: RJ
authors.lastname: Baker
authors.forename: D E
authors.initials: DE
authors.lastname: Mattson
authors.forename: J S
authors.initials: JS
authors.lastname: Guy
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000096:biosynthesis / Q000276:immunology*; D000956:Antigens, Viral / Q000032:analysis; D015153:Blotting, Western / Q000662:veterinary; D002645:Chickens / Q000276:immunology* / Q000821:virology; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D019084:Fluorescent Antibody Technique, Indirect / Q000662:veterinary; D006566:Herpesviridae Infections / Q000276:immunology / Q000662:veterinary* / Q000821:virology; D001352:Herpesvirus 1, Gallid / Q000276:immunology*; D051379:Mice; D011201:Poultry Diseases / Q000276:immunology* / Q000821:virology
chemical_list: D000911:Antibodies, Monoclonal; D000956:Antigens, Viral