Cytokeratin and vimentin expression in normal epithelium and benign lesions of the vocal cords.
The expression of cytokeratins and vimentin was studied immunohistochemically in normal epithelium and 12 benign lesions of the vocal cord with the use of a broad panel of monoclonal antibodies against cytokeratins and vimentin. Histology showed that the various lesions contained hyperkeratotic, hyperplastic and atrophic epithelium, irrespective of their clinical appearance. Especially the Ck profile of the (hyper)keratotic lesions was changed in comparison with the native epithelium. Increased expression of the keratinization marker Ck 10 was associated with decreased expression of the stratification markers Cks 4 and 13. Expression of the basal cell marker Ck 14 and hyperproliferation-associated Cks 16 and 17 was increased in all the benign lesions, except in atrophic epithelium. These expression patterns differ from those observed in malignant epithelial lesions. The latter show a marked expression of simple cell Cks and vimentin and more pronounced expression of hyperproliferation-associated markers than the benign lesions.
van der Velden
L A
LA
Department of Otorhinolaryngology, University Hospital Nijmegen, The Netherlands.
Schaafsma
H E
HE
Manni
J J
JJ
Link
M
M
Ruiter
D J
DJ
Ramaekers
F C
FC
Kuijpers
W
W
D000911:Antibodies, Monoclonal; D002294:Carcinoma, Squamous Cell / Q000648:ultrastructure; D004848:Epithelium / Q000648:ultrastructure; D006801:Humans; D007150:Immunohistochemistry; D007381:Intermediate Filament Proteins; D007633:Keratins / Q000032:analysis*; D007822:Laryngeal Neoplasms / Q000648:ultrastructure; D014746:Vimentin / Q000032:analysis*; D014827:Vocal Cords / Q000737:chemistry* / Q000648:ultrastructure
D000911:Antibodies, Monoclonal; D007381:Intermediate Filament Proteins; D014746:Vimentin; D007633:Keratins