[Comparative study of the kinetics of phospholipid liposome peroxidation, induced by hypochlorite and in the Fe2+ + ascorbate system].
The present study examined the kinetics of accumulation of lipid peroxidation products in phosphatidylcholine liposomes incubated with hypochlorite (HOCl/OCl-) or with FeSO4 in the presence of ascorbate. The incubation of liposomes with HOCl/OCl- led to the accumulation of primary (lipid hydroperoxides and conjugated dienes) and secondary (TBA-reactive substances) lipid peroxidation products identical to those generated in the presence of Fe2+/ascorbate. However the rate of the formation of lipid peroxidation products as well as the gain in their amount were sufficiently higher (4-150-fold and 3-6-fold, respectively) in the case of HOCl/OCl(-)-induced lipid peroxidation as compared to lipid peroxidation in the Fe2+/ascorbate system. The accumulation of lipid peroxidation products lasted until HOCl/OCl- was present in the reaction mixture. The data obtained support our hypothesis that hypochlorite produced by activated neutrophils and monocyte/macrophages may be reasonable for the initiation of lipid peroxidation in blood.
Panasenko
O M
OM
Institute of Medical Physics and Biophysics, University Leipzig, Germany.
Arnhold
J
J
Arnhold
K
K
Vladimirov
Iu A
IuA
Sergienko
V I
VI
D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology*; D002642:Chick Embryo; D005296:Ferrous Compounds / Q000494:pharmacology*; D006997:Hypochlorous Acid / Q000494:pharmacology*; D007700:Kinetics; D015227:Lipid Peroxidation / Q000187:drug effects*; D008081:Liposomes; D010713:Phosphatidylcholines / Q000378:metabolism*
D005296:Ferrous Compounds; D008081:Liposomes; D010713:Phosphatidylcholines; D006997:Hypochlorous Acid; D001205:Ascorbic Acid
Sravnitel'noe issledovanie kinetiki peroksidatsii fosfolipidnykh liposom, indutsirovannoĭ gipokhloritom i v sisteme Fe2+ + askorbat.