The level of pancreatic PLA2 receptor is closely associated with the proliferative state of rat uterine stromal cells.
Rat uterine stromal cells (U(III)) express pancreatic type PLA2 (PLA2-I) receptor and internalize the enzyme bound to receptors. Here, we investigate the proliferating effect and alterations in binding of PLA2-I. There is a dramatic decline in PLA2-I binding in U(III) cells as they progress from a non-confluent proliferating state (40,000 sites/cell) to a confluent state (1300 sites/cell). Intracellular concentration of PLA2-I changed with the alteration in binding, suggesting that regulation in the PLA2 binding capacity may have important implications in growth control mechanisms.
Tessier
C
C
Laboratoire de Physiologie-Pharmacodynamie, INSERM U.352, INSA 406, Villeurbanne, France.
Rossini
G P
GP
Pageaux
J F
JF
Cohen
H
H
Lagarde
M
M
Laugier
C
C
Fayard
J M
JM
D000818:Animals; D034341:Aristolochic Acids; D001665:Binding Sites; D002455:Cell Division; D002478:Cells, Cultured; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005260:Female; D010179:Pancreas / Q000378:metabolism; D010616:Phenanthrenes / Q000494:pharmacology; D010741:Phospholipases A / Q000037:antagonists & inhibitors / Q000378:metabolism* / Q000494:pharmacology; D054467:Phospholipases A2; D011485:Protein Binding; D051381:Rats; D011956:Receptors, Cell Surface / Q000378:metabolism*; D054507:Receptors, Phospholipase A2; D017154:Stromal Cells / Q000166:cytology / Q000378:metabolism; D013552:Swine; D014599:Uterus / Q000166:cytology* / Q000378:metabolism*
D034341:Aristolochic Acids; D004791:Enzyme Inhibitors; D010616:Phenanthrenes; D011956:Receptors, Cell Surface; D054507:Receptors, Phospholipase A2; C000228:aristolochic acid I; D010741:Phospholipases A; D054467:Phospholipases A2