Sensitivity to dietary obesity linked to a locus on chromosome 15 in a CAST/Ei x C57BL/6J F2 intercross.
Details of a new model of diet-dependent polygenic obesity are presented. CAST/Ei (Mus m. castaneus) mice remain lean after 12 weeks on a high-fat (32 kcal% fat) diet, while C57BL/6J mice become obese. The genes responsible for the obesity segregate in an F2 population derived from an intercross between CAST/Ei and C57BL/6J mice. Quantitative trait analysis, with simple sequence length polymorphisms (SSLPs) at loci previously linked to rodent obesities, identified a quantitative trait locus (QTL) on Chromosome (Chr) 15, accounting for approximately 9% of the variance in adiposity and 14% of the variance in mesenteric depot size. This locus appears to be at the same location as the dietary obesity-3 (Do3) locus controlling body fat content, which was previously identified in an F2 population derived from an SWR/J x AKR/J cross. This is also at the same location as the multigenic obesity-4 (Mob4) locus found in BSB mice, which display spontaneous polygenic obesity. Suggestive linkage also was found at loci close to the single gene mutations Ay on Chr 2 and tub on Chr 7.
York
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Obesity, Diabetes and Metabolism Section, Pennington Biomedical Research Center, 6400 Perkins Road, Louisiana State University, Baton Rouge, Louisiana 70808, USA.
Lei
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West
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D000273:Adipose Tissue / Q000033:anatomy & histology; D000704:Analysis of Variance; D000818:Animals; D001835:Body Weight; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D004041:Dietary Fats; D005260:Female; D008040:Genetic Linkage; D005819:Genetic Markers; D005838:Genotype; D008126:Lod Score; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL / Q000235:genetics; D008815:Mice, Inbred Strains / Q000235:genetics*; D005810:Multigene Family; D009765:Obesity / Q000209:etiology / Q000235:genetics*; D011110:Polymorphism, Genetic; D011995:Recombination, Genetic
D004041:Dietary Fats; D005819:Genetic Markers