Levodopa induces AP-1 and CREB DNA-binding activities in the rat striatum.
In order to elucidate the effect of levodopa and bromocriptine on the DNA-binding activities of transcription factors, AP-1 and CREB DNA-binding activities were investigated using gel-shift assay. Intraperitoneal administration of 100 mg/kg levodopa with 50 mg/kg benserazide in rats increased both AP-1 and CREB DNA-binding activities in the dorsolateral aspect of the striatum. The major proteins composing the increased AP-1 were JunB and JunD. Bromocriptine at doses of 2.5 and 5.0 mg/kg, however, did not increase these binding activities. Present results suggest that levodopa but not bromocriptine induces these transcription-regulating proteins in the striatum with normal dopaminergic functioning.
Kashihara
K
K
Department of Neurology, Okayama University Medical School, Japan.
Ishihara
T
T
Akiyama
K
K
Kuroda
S
S
Morimasa
T
T
Shomori
T
T
D000818:Animals; D000978:Antiparkinson Agents / Q000494:pharmacology*; D001971:Bromocriptine / Q000494:pharmacology; D050882:CREB-Binding Protein; D003342:Corpus Striatum / Q000187:drug effects* / Q000473:pathology; D004268:DNA-Binding Proteins / Q000235:genetics*; D004305:Dose-Response Relationship, Drug; D015870:Gene Expression / Q000187:drug effects; D007980:Levodopa / Q000494:pharmacology*; D008297:Male; D009687:Nuclear Proteins / Q000235:genetics*; D016755:Proto-Oncogene Proteins c-jun / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D015534:Trans-Activators; D018808:Transcription Factor AP-1 / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*
D000978:Antiparkinson Agents; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D016755:Proto-Oncogene Proteins c-jun; D015534:Trans-Activators; D018808:Transcription Factor AP-1; D014157:Transcription Factors; D001971:Bromocriptine; D007980:Levodopa; D050882:CREB-Binding Protein; C494365:Crebbp protein, rat