Naturally occurring anti-idiotypic antibodies to anti-phosphotyrosine in systemic lupus erythematosus interact with SRC-homology 2 domains.
We have recently identified in SLE sera naturally occurring anti-idiotypic antibodies against anti-phosphotyrosine antibodies. Analysis of immunochemical properties of these anti-idiotypic antibodies suggest that they are of beta/gamma type mimicking the antigen. The interaction between these anti-idiotypes and SH2 domains of various fusion proteins was analysed by immunoprecipitation and immunoblotting. Our data demonstrate that these anti-idiotypic antibodies specifically bind SH2 domains, with the highest affinity for SH2 domain of lck protein tyrosine kinase. The significance of this interaction is discussed.
Stefanescu
M
M
Department of Immunology, Cantacuzino Institute, Bucharest, Romania.
Onu
A
A
Matache
C
C
Ramos-Morales
F
F
Fischer
S
S
Szegli
G
G
D000888:Antibodies, Anti-Idiotypic / Q000502:physiology*; D019138:Antibodies, Blocking / Q000502:physiology; D000918:Antibody Specificity; D001323:Autoantibodies / Q000097:blood / Q000502:physiology*; D003429:Cross Reactions; D006801:Humans; D007113:Immunity, Innate; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*; D019860:Lymphocyte Specific Protein Tyrosine Kinase p56(lck); D019000:Phosphotyrosine / Q000276:immunology*; D018909:src Homology Domains / Q000276:immunology*; D019061:src-Family Kinases / Q000276:immunology
D000888:Antibodies, Anti-Idiotypic; D019138:Antibodies, Blocking; D001323:Autoantibodies; D019000:Phosphotyrosine; D019860:Lymphocyte Specific Protein Tyrosine Kinase p56(lck); D019061:src-Family Kinases