Monomeric chemically modified allergens: immunologic and physicochemical characterization.
Allergenic extracts (Der p, grass, and Parietaria) or single allergens such as Par j I (the major allergen of Parietaria) and ovalbumin (OA), a food allergen widely used in animal models, were chemically modified by reaction with potassium cyanate (KCNO), which transforms the epsilon-amino group of the lysine of proteinaceous allergens into ureido groups. KCNO-modified (carbamylated) allergens have low allergenic potency, as demonstrated in vitro (RAST inhibition) and in vivo (passive cutaneous anaphylaxis). When used to immunize rabbits, carbamylated allergens still induce IgG antibodies able to cross-react with native allergens (immunoblotting experiments). An interesting feature distinguishing carbamylated allergens from other chemically modified allergens is the preservation of the native monomeric dimension as demonstrated by SDS-PAGE analysis. Results are discussed from the perspective of clinical application of carbamylated allergens.
Mistrello
G
G
Department of Research, Laboratorio Famacautico Lofarma, Milan, Italy.
Brenna
O
O
Roncarolo
D
D
Zanoni
D
D
Gentili
M
M
Falagiani
P
P
D000485:Allergens / Q000737:chemistry* / Q000276:immunology*; D000818:Animals; D003485:Cyanates / Q000737:chemistry*; D015151:Immunoblotting; D007073:Immunoglobulin E / Q000096:biosynthesis; D051379:Mice; D008807:Mice, Inbred BALB C; D010323:Passive Cutaneous Anaphylaxis; D011817:Rabbits; D011852:Radioallergosorbent Test; D051381:Rats; D017207:Rats, Sprague-Dawley; D014614:Vaccines, Synthetic
D000485:Allergens; D003485:Cyanates; D014614:Vaccines, Synthetic; D007073:Immunoglobulin E; C024835:potassium cyanate