Phosphatidylserine-mediated adhesion of T-cells to endothelial cells.
Phosphatidylserine was exposed on the surface of human umbilical endothelial cells (ECV304) a few minutes after adding thrombin in vitro, as monitored by prothrombinase assays with and without annexin V. Jurkat T cells adhered to the thrombin-treated cells. The adhesion was inhibited by annexin V, indicating that it was mediated by exposed phosphatidylserine on the endothelial cells.
Qu
J
J
Department of Cellular Physiology, Babraham Institute, Cambridge, UK.
Conroy
L A
LA
Walker
J H
JH
Wooding
F B
FB
Lucy
J A
JA
D017304:Annexin A5 / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004730:Endothelium, Vascular / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D010718:Phosphatidylserines / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000502:physiology*; D013917:Thrombin / Q000494:pharmacology*; D013925:Thromboplastin / Q000032:analysis
D017304:Annexin A5; D010718:Phosphatidylserines; D013925:Thromboplastin; D013917:Thrombin