[Construction and analysis of transgenic plants of Nicotiana tabacum L. expressing a bacterial gene for beta-1,3-glucanase. II. Transgenic tobacco plants expressing the bacterial beta-glucanase gene from Clostridium thermocellum,--a model for studying the differential expression of stress response-related genes].
The modified hybrid beta-1,3-glucanase gene (glc) of Clostridium thermocellum was expressed in tobacco Nicotiana tabacum. The glc gene was cloned into two plasmids, pC27-glc and pC29-glc, in which its expression was controlled by the TR2' promoter of the 2' gene of T-DNA and the rbcS promoter of Arabidopsis, respectively. These constructions were used for transformation of agrobacteria followed by transfer into plants. In transformed plants, each plasmid caused a high level of activity of thermostable bacterial glucanase not observed in reference plants. The plants obtained were used to study activation of some defense-related genes induced by their interaction with either tobacco mosaic virus (TMV) or a pathogenic fungus.
Darbinian
N S
NS
Popov
Iu G
IuG
Mochul'skiĭ
A V
AV
Oming
D
D
Piruzian
E S
ES
Vasilevko
V T
VT
D003001:Cloning, Molecular; D003013:Clostridium / Q000235:genetics*; D005658:Fungi / Q000502:physiology; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D018506:Gene Expression Regulation, Plant / Q000502:physiology*; D043326:Glucan 1,3-beta-Glucosidase; D018869:Heat-Shock Response / Q000235:genetics; D008957:Models, Genetic; D030821:Plants, Genetically Modified; D010947:Plants, Toxic; D010957:Plasmids; D014026:Nicotiana / Q000235:genetics*; D014027:Tobacco Mosaic Virus / Q000502:physiology; D001617:beta-Glucosidase / Q000235:genetics*
D001617:beta-Glucosidase; D043326:Glucan 1,3-beta-Glucosidase
Konstruirovanie i analiz transgennykh rasteniĭ Nicotiana tabacum L. s ékspressiruiushchimsia bakterial'nym genom beta-1,3-gliukanazy. II. Transgennye rasteniia tabaka, ékspressiruiushchie bakterial'nye gen beta-gliukanazy iz Clostridium thermocellum,--model' dlia izucheniia differentsial'noĭ ékspressii genov stressovogo otveta.