Collagen-binding activity of Prevotella intermedia measured by a microtitre plate adherence assay.
The ability of Prevotella intermedia to bind type I collagen was investigated. A simple method in which bacterial cells were allowed to attach to collagen-coated microtitre plate wells was used to characterize the activity. All strains of P. intermedia tested, as well as those of the closely related species Prevotella nigrescens, showed a capacity to attach to the collagen film. Exponential-phase cultures of P. intermedia demonstrated a greater binding capacity than older cells. Attachment to the collagen film was inhibited by the presence of EDTA, type I and IV collagen, denatured collagen (gelatin), fibrinogen or fibronectin. Pretreatment of bacterial cells with heat (60 degrees C, 30 min) or proteinase K also inhibited the binding. The collagen-binding activity could be solubilized from the bacterial cell surface by incubation with Zwittergent 3-14, a zwitterionic detergent. The collagen-binding capacity of P. intermedia demonstrated in the present study represents a mechanism of colonization allowing these bacteria to attach to a tissue matrix.
Grenier
Daniel
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Groupe de Recherche en Ecologie Buccale, FacultÃ© de MÃ©decine Dentaire, UniversitÃ© Laval, Sainte-Foy, QuÃ©bec, G1K7P4Canada.
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000032:analysis* / Q000302:isolation & purification / Q000378:metabolism; D001431:Bacteriological Techniques / Q000295:instrumentation; D003094:Collagen / Q000378:metabolism*; D003902:Detergents / Q000494:pharmacology; D005340:Fibrinogen / Q000378:metabolism; D005353:Fibronectins / Q000378:metabolism; D005780:Gelatin / Q000378:metabolism; D006358:Hot Temperature; D016023:Integrins / Q000032:analysis* / Q000302:isolation & purification / Q000378:metabolism; D008969:Molecular Sequence Data; D018720:Prevotella / Q000187:drug effects / Q000378:metabolism; D018713:Prevotella intermedia / Q000187:drug effects / Q000378:metabolism* / Q000472:pathogenicity; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology; D038981:Receptors, Collagen; D015203:Reproducibility of Results; D014774:Virulence
D001426:Bacterial Proteins; D003902:Detergents; D005353:Fibronectins; D016023:Integrins; D000644:Quaternary Ammonium Compounds; D038981:Receptors, Collagen; C048256:zwittergent 3-14; D005780:Gelatin; D005340:Fibrinogen; D003094:Collagen