title: Induction and involvement of endogenous IGF-I in pancreas regeneration after partial pancreatectomy in the dog.
abstract: To elucidate whether and how IGF-I is involved in the regeneration of the pancreas after partial pancreatectomy, IGF-I mRNA expression, IGF-I protein synthesis, ornithine decarboxylase (ODC) activity and DNA replication in the remnant pancreas were determined in the dog. After pancreatectomy, IGF-I mRNA expression was remarkably enhanced in the remnant pancreas, showing the maximal value at post-operative day (POD) 1. Subsequently, IGF-I synthesis in the tissue was significantly stimulated at POD 2, and its maximal concentration was observed at POD 3. Following IGF-I synthesis, ODC activity was induced and its maximal activity was also obtained at POD 3. Finally, DNA replication was induced in the remnant pancreas, and its maximal level was observed at POD 5. These responses in the remnant pancreatic tissue to partial pancreatectomy were greatly enhanced as the resection rate was increased up to 95%. Positive correlations were observed between IGF-I concentrations in the remnant pancreas and the activities of ODC and DNA synthesis in the tissue after 95% pancreatectomy. These results suggest that the gene expression of IGF-I is rapidly induced in the remnant pancreas after partial pancreatectomy, and subsequently synthesized endogenous IGF-I peptides may stimulate ODC and other cell growth-related activities in the tissue in paracrine and/or autocrine manners eventually to induce DNA replication and tissue regeneration.
authors.affiliation: Department of Biochemistry, Mie University, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Hayakawa
authors.forename: Y
authors.initials: Y
authors.lastname: Kawarada
authors.forename: R
authors.initials: R
authors.lastname: Mizumoto
authors.forename: H
authors.initials: H
authors.lastname: Hibasami
authors.forename: M
authors.initials: M
authors.lastname: Tanaka
authors.forename: K
authors.initials: K
authors.lastname: Nakashima
mesh_terms: D000818:Animals; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D004261:DNA Replication; D004285:Dogs; D015870:Gene Expression; D007334:Insulin-Like Growth Factor I / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D008969:Molecular Sequence Data; D009955:Ornithine Decarboxylase / Q000378:metabolism; D010179:Pancreas / Q000378:metabolism / Q000502:physiology* / Q000601:surgery; D010180:Pancreatectomy; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis; D012038:Regeneration / Q000502:physiology*; D013997:Time Factors
chemical_list: D017931:DNA Primers; D012333:RNA, Messenger; D007334:Insulin-Like Growth Factor I; D009955:Ornithine Decarboxylase