title: Defective calcium increase and inositol phosphate production in anti-CD3-stimulated lymphocytes of alcoholics without progressive liver disease.
abstract: Intracellular free calcium concentration, phosphoinositide turnover, and inositol phosphate production were analyzed in peripheral blood lymphocytes from seven well-nourished alcoholic patients without severe acute or chronic liver disease, before and after stimulation with anti-CD3 antibody. Seven comparable nondrinkers were studied as controls. A lower increase in intracellular free calcium concentration was detected in alcoholics, after anti-CD3 stimulation of lymphocytes, than in control subjects. Lymphocyte activation generated inositol phosphates in both controls and alcoholics, but inositol phosphate production was significantly lower in alcoholics. The agreement between these findings indicates that the reduction in inositol phosphates is one of the most important events in the early phases of lymphocyte activation in alcoholics.
authors.affiliation: Department of Biochemistry G. Moruzzi, University of Bologna, Italy.
authors.forename: G F
authors.initials: GF
authors.lastname: Stefanini
authors.forename: E
authors.initials: E
authors.lastname: Castelli
authors.forename: F G
authors.initials: FG
authors.lastname: Foschi
authors.forename: A
authors.initials: A
authors.lastname: Terzi
authors.forename: P L
authors.initials: PL
authors.lastname: Biagi
authors.forename: A
authors.initials: A
authors.lastname: Bordoni
authors.forename: M
authors.initials: M
authors.lastname: Celadon
authors.forename: S
authors.initials: S
authors.lastname: Hrelia
mesh_terms: D000328:Adult; D000437:Alcoholism / Q000276:immunology* / Q000534:rehabilitation; D017252:CD3 Complex / Q000276:immunology*; D002118:Calcium / Q000097:blood*; D005260:Female; D006801:Humans; D007295:Inositol Phosphates / Q000097:blood*; D008108:Liver Diseases, Alcoholic / Q000276:immunology; D008213:Lymphocyte Activation / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D011948:Receptors, Antigen, T-Cell / Q000276:immunology; D012016:Reference Values
chemical_list: D017252:CD3 Complex; D007295:Inositol Phosphates; D011948:Receptors, Antigen, T-Cell; D002118:Calcium