[The radiobiologic characteristics of DNA polymerase beta in hepatomas].
DNA polymerase beta activity, its content and gene transcription levels in SMMC-LTNM hepatoma were investigated, using 3H-TTP incorporation, immunocytochemistry and cytoplasmic dot hybridization, respectively. The relations between the biological properties of the enzyme and DNA repair synthesis induced by gamma-ray irradiation were also studied. It was found that DNA polymerase beta activity, its content and the amount of its mRNA were much higher in hepatoma than those in normal hepatocytes (P < 0.01). Following whole-body irradiation of the nude mouse bearing SMMC-LTNM with 2 Gy of gamma ray, the polymerase beta activity in hepatoma increased temporarily and the gene transcription of the enzyme seemed to be more active. DNA polymerase beta participated in DNA repair synthesis and this effect was different between hepatoma and hepatocyte because of the biological differences of DNA polymerase beta. The results presented here indicated that DNA polymerase beta could affect radiation damage and radiotherapy of cancer.
Cai
J
J
Department of Radiation Medicine, Second Military Medical University, Shanghai.
Zheng
X
X
Luo
C
C
D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000201:enzymology*; D004256:DNA Polymerase I / Q000235:genetics / Q000378:metabolism*; D004260:DNA Repair; D004273:DNA, Neoplasm / Q000528:radiation effects; D006801:Humans; D008113:Liver Neoplasms / Q000201:enzymology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D012333:RNA, Messenger / Q000096:biosynthesis*; D014916:Whole-Body Irradiation
D004273:DNA, Neoplasm; D012333:RNA, Messenger; D004256:DNA Polymerase I