A low-copy number plasmid mediating beta-lactamase production by Xanthomonas maltophilia.
To delineate the mechanisms contributing to the high level of antimicrobial resistance often demonstrated by Xanthomonas maltophilia, plasmid DNA was isolated from 5 clinical isolates and analyzed. Purified plasmid DNA from a single isolate contained a 6.5 kb plasmid (pXM222) and a 5.6 kb plasmid, (pTHB). Transformation of pTHB into E. coli HB101 resulted in the expression of resistance to all penicillins tested and cefazolin.
Kelly
M D
MD
Cardeza Foundation for Hematological Research, Thomas Jefferson University, Philadelphia, PA, USA.
Mortensen
J E
JE
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D004587:Electrophoresis, Agar Gel; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics; D008826:Microbial Sensitivity Tests; D010957:Plasmids / Q000302:isolation & purification; D015183:Restriction Mapping; D014169:Transformation, Bacterial; D014974:Xanthomonas / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D018440:beta-Lactam Resistance / Q000235:genetics*; D001618:beta-Lactamases / Q000096:biosynthesis*; D047090:beta-Lactams
D000900:Anti-Bacterial Agents; D047090:beta-Lactams; D001618:beta-Lactamases