title: Serological and genomic characterization of porcine rotaviruses in Thailand: detection of a G10 porcine rotavirus.
abstract: A total of 557 fecal specimens collected from piglets with diarrhea in Thailand were examined for rotavirus RNA by polyacrylamide gel electrophoresis. Twenty-three, one, and two samples were positive for group A, group B, and group C rotaviruses, respectively. Two samples exhibited two segments found in picobirnavirus RNA. RNA electropherotyping of 23 group A rotaviruses showed that they were classified into five patterns. By serotyping by enzyme-linked immunosorbent assay and PCR, viruses in 3 and 14 specimens were found to be serotype G3 and serotype G10, respectively. For one specimen, containing a serotype G10 virus (strain P343), virus was isolated in MA-104 cells, and the nucleotide sequences of the VP7 and VP4 genes were determined. Comparative sequence analysis and cross-neutralization tests showed that strain P343 has B223-like G10 and UK-like P7 serotype (or VP4 genotype 5) specificities. Rotaviruses having such antigenic specificities have not been detected in piglets. Thus, the interspecies transmission of rotaviruses between cows and pigs was suggested.
authors.affiliation: Department of Medical Sciences, National Institute of Health, Nonthaburi, Thailand.
authors.forename: Y
authors.initials: Y
authors.lastname: Pongsuwanna
authors.forename: K
authors.initials: K
authors.lastname: Taniguchi
authors.forename: M
authors.initials: M
authors.lastname: Chiwakul
authors.forename: T
authors.initials: T
authors.lastname: Urasawa
authors.forename: F
authors.initials: F
authors.lastname: Wakasugi
authors.forename: C
authors.initials: C
authors.lastname: Jayavasu
authors.forename: S
authors.initials: S
authors.lastname: Urasawa
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000956:Antigens, Viral; D001483:Base Sequence; D002213:Capsid / Q000235:genetics; D036022:Capsid Proteins; D002417:Cattle; D004279:DNA, Viral / Q000235:genetics; D003967:Diarrhea / Q000662:veterinary / Q000821:virology; D005243:Feces / Q000821:virology; D005814:Genes, Viral; D016679:Genome, Viral; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification; D012401:Rotavirus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D012400:Rotavirus Infections / Q000635:transmission / Q000662:veterinary / Q000821:virology; D012703:Serotyping; D013045:Species Specificity; D013552:Swine / Q000821:virology*; D013553:Swine Diseases / Q000821:virology; D013785:Thailand
chemical_list: D000956:Antigens, Viral; D036022:Capsid Proteins; D004279:DNA, Viral; D012367:RNA, Viral; C060339:VP4 protein, Rotavirus; C050779:VP7 protein, Rotavirus