Application of capillary zone electrophoresis for analyzing biotin in pharmaceutical formulations--a comparative study.
The detection limit and reproducibility of capillary zone electrophoresis (CZE) measurement of biotin were compared to those of a spectrophotometric method and those of the determination of the sulphur content by combustion of the biotin sample followed by coulometric titration of the formed SO2. Drug analysis showed that all three methods gave consistent results and were suitable for the determination of biotin. CZE was found to be the best method for the determination of pharmaceutical formulations containing biotin because of its high separation efficiency, short analysis time, ease of instrumentation and sample preconditioning, and good precision.
Schiewe
J
J
Institute of Pharmaceutics and Biopharmaceutics, Martin-Luther-University, Halle/Saale, Germany.
Göbel
S
S
Schwarz
M
M
Neubert
R
R
D001710:Biotin / Q000032:analysis*; D002626:Chemistry, Pharmaceutical; D019075:Electrophoresis, Capillary; D015203:Reproducibility of Results
D001710:Biotin