[t(14:18) chromosomal translocation and over expression of bcl-2 protein in follicular type non-Hodgkin's lymphoma].
Polymerase chain reaction (PCR) and immunohistochemical staining methods were used to detect the bcl-2/IgH fusion gene [t(14:18)] and the overexpression of bcl-2 protein in 28 cases of follicular lymphomas and 18 cases of reactive follicular hyperplasia. The results showed that the bcl-2/IgH fusion gene was present in 57% of follicular lymphomas and that this translocation was also present in 33% of the reactive follicular hyperplasia cases. The over expression of bcl-2 protein was found in 78% of follicular lymphomas, but none in follicular hyperplasia, therefore bcl-2 protein staining may be a useful immunohistochemical method to differentiate follicular lymphoma and follicular hyperplasia.
Wang
J
J
Department of Pathology, West China University of Medical Sciences, Chengdu.
Li
G
G
Liu
Z
Z
D002883:Chromosomes, Human, Pair 14; D002887:Chromosomes, Human, Pair 18; D003001:Cloning, Molecular; D004273:DNA, Neoplasm / Q000235:genetics; D015870:Gene Expression; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D008224:Lymphoma, Follicular / Q000235:genetics* / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis* / Q000235:genetics; D014178:Translocation, Genetic
D004273:DNA, Neoplasm; D007143:Immunoglobulin Heavy Chains; D019253:Proto-Oncogene Proteins c-bcl-2