Activation of recombinant human protein C.
We have produced recombinant human Protein C (rHPC) in the milk of transgenic swine. After purification, we have analyzed the interaction of teh zymogen with Protac, thrombin/thrombomodulin and thrombin alone. The amidolytic and anticoagulant activities of rAPC after Protac activation were approximately 80% those of its human plasma counterpart. Upon the excision of the activation peptide by thrombin/thrombomodulin complex, both the natural and recombinant activation products had similar enzymatic and biological activities. This observation can be attributed to the difference in the mechanism of action between the two activators and structural differences between HPC and rHPC.
Lee
T K
TK
Holland Laboratory, American Red Cross, Rockville, MD 20855, USA.
Bangalore
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N
Velander
W
W
Drohan
W N
WN
Lubon
H
H
D000818:Animals; D030801:Animals, Genetically Modified; D000925:Anticoagulants / Q000819:agonists / Q000378:metabolism* / Q000494:pharmacology; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides / Q000494:pharmacology; D011486:Protein C / Q000819:agonists / Q000378:metabolism* / Q000494:pharmacology; D011994:Recombinant Proteins / Q000819:agonists / Q000378:metabolism / Q000494:pharmacology; D013552:Swine; D013917:Thrombin / Q000737:chemistry; D018180:Thrombomodulin / Q000737:chemistry
D000925:Anticoagulants; D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides; D011486:Protein C; D011994:Recombinant Proteins; D018180:Thrombomodulin; C049647:snake venom protein C activator; D013917:Thrombin