[Viability and antigenic changes in the skin after preservation under different temperatures].
Oxygen consumption and succinate dehydrogenase contents were determined to compare mean viabilities (%) of human cadaver and guinea pig skin stored under different temperature: 4 C, -20 C, -80 C, and -196 C. The results showed that the mean viability (%) of skin cryopreserved under -196 C was superior to those under 4 C, -20 C, and -80 C (70.8%, 61% respectively for -196 C, P < 0.05 or 0.01). Microimmunoelectrophoresis and rocket immunoelectrophoresis were performed for related antigens and antibodies. The results of microimmunoelectrophoresis showed that the antigenicities were different for four groups. Antigenicity of fresh skin was highest. The higher the storage temperature, the lower the antigenicity of skin was.
Ju
X
X
304th Hospital of People's Liberation Army, Beijing.
Zhu
Z
Z
Li
C
C
D000818:Animals; D015925:Cryopreservation; D000939:Epitopes; D006168:Guinea Pigs; D006801:Humans; D010101:Oxygen Consumption; D012867:Skin / Q000276:immunology; D013385:Succinate Dehydrogenase / Q000378:metabolism; D013696:Temperature; D014021:Tissue Preservation
D000939:Epitopes; D013385:Succinate Dehydrogenase