title: The interference of gingival cell cultures with growth of selected bacteria.
abstract: The aim of the present study was to analyze the interference of oral tissue cells or cell lines (effector cells) with growth of reference bacteria, and furthermore to investigate whether cells derived from different individuals differ in such activity. The reference bacteria were Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus mitis, Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, and Fusobacterium nucleatum. The effector cells used were gingival fibroblasts (GF) from 21 periodontally involved persons, gingival epithelial cells (E) from 2 such persons, HeLa cells (HeLa), and an amnion cell line (Amnion). The cells were cultivated and their supernatants tested for antibacterial activity in a Bioscreen robot analyzer (Labsystems, Finland). Results suggest that the antibacterial activity of each tested primary cell line of tissue had its own profile depending on cell type and donor, and that the composition of oral microbiota was influenced by oral cells, which might, in turn, contribute to the variations in the pathogenesis of periodontal diseases.
authors.affiliation: Department of Periodontology, Umeå University, Sweden.
authors.forename: A
authors.initials: A
authors.lastname: Johansson
authors.forename: A
authors.initials: A
authors.lastname: Bergenholtz
authors.forename: S E
authors.initials: SE
authors.lastname: Holm
mesh_terms: D016976:Aggregatibacter actinomycetemcomitans / Q000254:growth & development; D001419:Bacteria / Q000254:growth & development*; D018929:Cell Culture Techniques; D002455:Cell Division; D015169:Colony Count, Microbial / Q000295:instrumentation / Q000379:methods; D003470:Culture Media / Q000494:pharmacology; D016967:Fusobacterium nucleatum / Q000254:growth & development; D005881:Gingiva / Q000166:cytology / Q000382:microbiology*; D006367:HeLa Cells; D006801:Humans; D016966:Porphyromonas gingivalis / Q000254:growth & development; D013211:Staphylococcus aureus / Q000254:growth & development; D013212:Staphylococcus epidermidis / Q000254:growth & development; D013291:Streptococcus / Q000254:growth & development
chemical_list: D003470:Culture Media