Some effects of a low sodium intake on the expression of P450 aldosterone synthase in the hamster adrenal cortex: immunoblotting, immunofluorescent and immuno-gold electron microscopic studies.
In the current work we studied the effects of a low sodium intake on P450 aldosterone synthase (P450aldo) in the adrenal cortex of male hamsters by Western blotting analysis. We also investigated the zonal distribution of P450aldo with a specific antibody using immunofluorescence and immuno-gold electron microscopy. Western blotting analysis revealed a progressive induction of P450aldo in the adrenals of hamsters kept on a low sodium diet, with two-, four- and eightfold increases after 2, 4 and 21 days on the diet. Immunofluorescence microscopy showed that P450aldo was confined to the zona glomerulosa (ZG) cells. Electron microscopy showed P450aldo to be located in the mitochondria of ZG cells. When hamsters were maintained on a low sodium intake for 2, 11 and 21 days, P450aldo was still found only in the ZG; the ZG appeared either unchanged or sometimes slightly enlarged. Moreover, at days 11 and 21, the intensity of the immunofluorescent signal was much stronger in the ZG of hamsters on the low sodium intake than in controls. Hence, immunocytochemistry using the colloidal-gold technique showed P450aldo to be more abundant in the mitochondria of the experimental animals than in controls. To conclude, P450aldo is present only in the ZG of hamster adrenals and sodium restriction appears to induce its expression by stimulating production within individual ZG cells rather than by stimulating a proliferation of the ZG cells.
LeHoux
J G
JG
Department of Biochemistry, Sherbrooke University, Quebec, Canada.
Lefebvre
A
A
Ducharme
L
L
Lehoux
J
J
Martel
D
D
Brière
N
N
D000302:Adrenal Cortex / Q000201:enzymology*; D000818:Animals; D015153:Blotting, Western; D006224:Cricetinae; D019405:Cytochrome P-450 CYP11B2; D003577:Cytochrome P-450 Enzyme System / Q000032:analysis / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D008297:Male; D008647:Mesocricetus; D016253:Microscopy, Immunoelectron; D008928:Mitochondria / Q000201:enzymology; D012964:Sodium / Q000008:administration & dosage*; D013252:Steroid 11-beta-Hydroxylase / Q000032:analysis / Q000378:metabolism*; D013997:Time Factors; D015384:Zona Glomerulosa / Q000201:enzymology
D003577:Cytochrome P-450 Enzyme System; D012964:Sodium; D019405:Cytochrome P-450 CYP11B2; D013252:Steroid 11-beta-Hydroxylase