Autonomic and sensory neuron cultures.
This chapter has provided a rather detailed protocol for the dissection, dissociation, and culture of autonomic and sensory neurons from the chicken embryo. These protocols are by no means absolute. Many other laboratories that routinely culture these neurons may use techniques that differ significantly from the ones detailed in this chapter. All of the protocols described in this chapter can also be applied to quail embryos, which develop more rapidly but are of comparable size to chicken embryos until about E9. The list of suppliers for the various reagents described in these protocols is also limited. Many other vendors of cell culture products are probably equally reliable.
Nishi
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Department of Cell and Developmental Biology, Oregon Health Sciences University, Portland 97201, USA.
D000818:Animals; D001341:Autonomic Nervous System / Q000166:cytology / Q000196:embryology*; D002478:Cells, Cultured; D002642:Chick Embryo; D003470:Culture Media; D004210:Dissection / Q000295:instrumentation / Q000379:methods; D005724:Ganglia / Q000196:embryology; D009474:Neurons; D009475:Neurons, Afferent
D003470:Culture Media