title: Increased expression of brain-derived neurotrophic factor but not neurotrophin-3 mRNA in rat brain after cortical impact injury.
abstract: Levels of brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NT3) mRNA expression were measured in a rodent model of traumatic brain injury (TBI) following unilateral injury to the cerebral cortex. To obtain reliable data on the co-expression of neurotrophin genes, adjacent coronal sections from the same rat brains were hybridized in situ with BDNF and NT3 cRNA probes. BDNF mRNA increased at 1,3, and 5 hr after unilateral cortical injury in the cortex ipsilateral to the injury site and bilaterally in the dorsal hippocampus. NT3 mRNA did not change significantly following injury. Our results suggest that TBI produces rapid increases in BDNF mRNA expression in rat brain without changes in NT3 mRNA expression, a finding which differs from studies of ischemia and seizures. It is possible that increased levels of BDNF mRNA rather than NT3 are important components of pathophysiological responses to TBI.
authors.affiliation: Department of Neurosurgery, University of Texas Health Sciences Center at Houston 77030, USA.
authors.forename: K
authors.initials: K
authors.lastname: Yang
authors.forename: J R
authors.initials: JR
authors.lastname: Perez-Polo
authors.forename: X S
authors.initials: XS
authors.lastname: Mu
authors.forename: H Q
authors.initials: HQ
authors.lastname: Yan
authors.forename: J J
authors.initials: JJ
authors.lastname: Xue
authors.forename: Y
authors.initials: Y
authors.lastname: Iwamoto
authors.forename: S J
authors.initials: SJ
authors.lastname: Liu
authors.forename: C E
authors.initials: CE
authors.lastname: Dixon
authors.forename: R L
authors.initials: RL
authors.lastname: Hayes
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001930:Brain Injuries / Q000378:metabolism* / Q000503:physiopathology; D019208:Brain-Derived Neurotrophic Factor; D002540:Cerebral Cortex / Q000293:injuries / Q000378:metabolism*; D018891:Dentate Gyrus / Q000378:metabolism*; D007839:Functional Laterality; D006624:Hippocampus / Q000378:metabolism*; D008297:Male; D009129:Muscle Tonus; D009414:Nerve Growth Factors / Q000096:biosynthesis*; D009419:Nerve Tissue Proteins / Q000096:biosynthesis*; D020933:Neurotrophin 3; D009928:Organ Specificity; D011187:Posture; D017966:Pyramidal Cells / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D012018:Reflex; D014158:Transcription, Genetic
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins; D020933:Neurotrophin 3; D012333:RNA, Messenger