title: Effect of interleukin-1 receptor antagonist (IL-1RA) on histamine and serotonin release by rat basophilic leukemia cells (RBL-2H3) and peritoneal mast cells.
abstract: Recently, it has been appreciated that cultured mast cells are significant sources of cytokines. However, the role of interkeukin-1 (IL-1) on mast cells and/or basophil degranulation is still unclear. In this report we provide evidence that rat basophilic leukemia cells (RBLC) cultured with a natural inhibitor of IL-1, interleukin-1 receptor antagonist (IL-1RA) (500 ng/ml) for 48 h, strongly inhibited the spontaneous release of serotonin (5HT) and histamine (from 22.50 to 43.49%), compared to untreated cells (control). When IL-1RA-treated and untreated RBLC were stimulated with a secretagogue (anti-IgE), no difference was found in the percent of 5HT and histamine release. Moreover, in another set of experiments using rat peritoneal mast cells (RPMC) treated and untreated with IL-1RA, we found that IL-1RA did not affect the release of 5HT or histamine, even when the secretagogue anti-IgE or compound 48/80 (C48/80) were used. The present studies describe an additional biological activity of IL-1RA, inhibiting histamine and 5HT release from RBLC cultures.
authors.affiliation: Immunology Division, University of Chieti, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Conti
authors.forename: W
authors.initials: W
authors.lastname: Bouchet
authors.forename: C
authors.initials: C
authors.lastname: Feliciani
authors.forename: S
authors.initials: S
authors.lastname: Mammarella
authors.forename: L
authors.initials: L
authors.lastname: Kudchadker
authors.forename: R C
authors.initials: RC
authors.lastname: Barbacane
authors.forename: M
authors.initials: M
authors.lastname: Reale
authors.forename: I
authors.initials: I
authors.lastname: Haggag
authors.forename: G
authors.initials: G
authors.lastname: Bruneau
authors.forename: J
authors.initials: J
authors.lastname: Thibault
authors.forename: T C
authors.initials: TC
authors.lastname: Theoharides
mesh_terms: D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D001483:Base Sequence; D015152:Blotting, Northern; D002460:Cell Line; D002470:Cell Survival; D002478:Cells, Cultured; D017931:DNA Primers; D006636:Histamine Release / Q000187:drug effects*; D006640:Histidine Decarboxylase / Q000096:biosynthesis*; D007073:Immunoglobulin E / Q000276:immunology; D053590:Interleukin 1 Receptor Antagonist Protein; D007375:Interleukin-1; D007700:Kinetics; D015471:Leukemia, Basophilic, Acute; D008407:Mast Cells / Q000187:drug effects / Q000502:physiology*; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction; D051381:Rats; D012701:Serotonin / Q000378:metabolism*; D012795:Sialoglycoproteins / Q000494:pharmacology*; D014407:Tumor Cells, Cultured; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology
chemical_list: D000906:Antibodies; D017931:DNA Primers; D053590:Interleukin 1 Receptor Antagonist Protein; D007375:Interleukin-1; D015345:Oligonucleotide Probes; D012795:Sialoglycoproteins; D012701:Serotonin; D007073:Immunoglobulin E; D003189:p-Methoxy-N-methylphenethylamine; D006640:Histidine Decarboxylase