[Immunohistochemical study of hepatitis C virus (HCV) antigen in liver tissue of patients with HCV infection].
Hepatitis C virus antigen (HCV Ag) and HCV-NS3 protein in paraffin-embedded liver tissue of patients with HCV infection were detected with immunohistochemical method by using polyclonal anti-HCV and monoclonal anti-HCV-NS3 for studying the expression of HCV in liver tissue. The results showed that the detection rate of HCV Ag and HCV-NS3 were 38.3% and 30.0% respectively. All the specimens with HCV-NS3 positive tissue were also those with HCV Ag positive. The stained substance located in the cytoplasm of liver cell, and the stained cells distributed scattered, clustered or diffuse in liver lobular. No obvious infiltration or necrosis was observed around the positive cells. There was obvious difference of the detection rate between different models of serum HCV markers. These results provided some help in understanding the pathogenesis of HCV.
Zhao
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X
Clinical Immunology Research Unit, Tongji Hospital, Tongji Medical University, Wuhan.
Yang
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D
Li
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L
D000293:Adolescent; D000328:Adult; D000911:Antibodies, Monoclonal; D015415:Biomarkers / Q000032:analysis; D005260:Female; D016174:Hepacivirus / Q000276:immunology*; D006526:Hepatitis C / Q000276:immunology*; D018936:Hepatitis C Antigens / Q000032:analysis*; D006801:Humans; D007150:Immunohistochemistry; D008099:Liver / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D017361:Viral Nonstructural Proteins / Q000032:analysis; D012697:Serine Endopeptidases; D043583:Nucleoside-Triphosphatase; D053487:DEAD-box RNA Helicases; D000085242:Viral Proteases
D000911:Antibodies, Monoclonal; D015415:Biomarkers; D018936:Hepatitis C Antigens; D017361:Viral Nonstructural Proteins; C084422:NS3 protein, hepatitis C virus; D012697:Serine Endopeptidases; D043583:Nucleoside-Triphosphatase; D053487:DEAD-box RNA Helicases; D000085242:Viral Proteases