title: Mdm-2 is not induced by p53 in human keratinocytes in vivo.
abstract: Normal p53 protein protects the genome after DNA damage by delaying replication and allowing DNA repair. mdm-2 is a recently discovered protein that controls p53 activity by binding to and inactivating p53 protein. A negative feedback loop has been suggested in which p53 induces mdm-2 expression. We have shown that doses of ultraviolet B radiation that cause mild sunburn clinically, produce thymine dimers in keratinocytes detectable by immunocytochemistry. This causes an elevation of p53 protein without a concomitant p53-mediated induction of mdm-2.
authors.affiliation: University Department of Dermatology, Glasgow, UK.
authors.forename: A D
authors.initials: AD
authors.lastname: Burden
authors.forename: G I
authors.initials: GI
authors.lastname: Stables
authors.forename: I
authors.initials: I
authors.lastname: Campbell
authors.forename: W
authors.initials: W
authors.lastname: Thomson
authors.forename: R M
authors.initials: RM
authors.lastname: MacKie
mesh_terms: D004249:DNA Damage / Q000502:physiology*; D005246:Feedback; D006801:Humans; D015603:Keratinocytes / Q000378:metabolism*; D009687:Nuclear Proteins; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D051736:Proto-Oncogene Proteins c-mdm2; D012867:Skin / Q000528:radiation effects; D013997:Time Factors; D016159:Tumor Suppressor Protein p53 / Q000502:physiology*; D014466:Ultraviolet Rays
chemical_list: D009687:Nuclear Proteins; D011518:Proto-Oncogene Proteins; D016159:Tumor Suppressor Protein p53; C497206:MDM2 protein, human; D051736:Proto-Oncogene Proteins c-mdm2