[Bioantimutagenic effect of the culture fluid of Streptococcus faecalis on mutagenesis induced by 2-nitrofluorene in Salmonella typhimurium TA 1538 and TA 98].
Streptococcus faecalis growing in rich organic media or synthetic medium containing 0.1% trypton and 0.05% yeast extract was shown to form substances with a bioantimutagenic activity against the mutagenicity of 2-nitrofluorene and 4-nitroquinoline-1-oxide, which induce frameshift and base-pair substitution mutations, respectively. The maximum accumulation of antimutagens in the medium was observed within 16-24 h of growth. The modified Ames test showed that the culture liquid of Str. faecalis possessed a higher antimutagenic activity for Salmonella typhimurium strain TA 1538 than for strain TA 98 with an enhanced SOS response. Fractionation of the culture liquid revealed that the bioantimutagenic activity is localized in the fraction of peptides with molecular masses of less than 12 kDa.
Vorob'eva
L I
LI
Cherdyntseva
T A
TA
Abilev
S K
SK
D015112:4-Nitroquinoline-1-oxide; D016587:Antimutagenic Agents / Q000494:pharmacology*; D017077:Culture Media, Conditioned; D013293:Enterococcus faecalis / Q000378:metabolism*; D005449:Fluorenes / Q000037:antagonists & inhibitors*; D016368:Frameshift Mutation / Q000187:drug effects*; D008970:Molecular Weight; D009153:Mutagens; D010455:Peptides / Q000378:metabolism; D013014:SOS Response, Genetics / Q000187:drug effects; D012486:Salmonella typhimurium / Q000187:drug effects* / Q000235:genetics; D013045:Species Specificity
D016587:Antimutagenic Agents; D017077:Culture Media, Conditioned; D005449:Fluorenes; D009153:Mutagens; D010455:Peptides; C019499:2-nitrofluorene; D015112:4-Nitroquinoline-1-oxide
Bioantimutagennoe deĭstvie kul'tural'noĭ zhidkosti Streptococcus faecalis protiv mutageneza, indutsirovannogo 2-nitrofluorenom u Salmonella typhimurium TA 1538 i TA98.