title: The Caenorhabditis elegans sel-1 gene, a negative regulator of lin-12 and glp-1, encodes a predicted extracellular protein.
abstract: The Caenorhabditis elegans lin-12 and glp-1 genes encode members of the LIN-12/NOTCH family of receptors. The sel-1 gene was identified as an extragenic suppressor of a lin-12 hypomorphic mutant. We show in this report that the sel-1 null phenotype is wild type, except for an apparent elevation in lin-12 and glp-1 activity in sensitized genetic backgrounds, and that this genetic interaction seems to be lin-12 and glp-1 specific. We also find that sel-1 encodes a predicted extracellular protein, with a domain sharing sequence similarity to predicted proteins from humans and yeast. SEL-1 may interact with the LIN-12 and GLP-1 receptors and/or their respective ligands to down-regulate signaling.
authors.affiliation: Department of Molecular Biology, Princeton University, New Jersey 08544, USA.
authors.forename: B
authors.initials: B
authors.lastname: Grant
authors.forename: I
authors.initials: I
authors.lastname: Greenwald
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017173:Caenorhabditis elegans / Q000235:genetics* / Q000502:physiology; D029742:Caenorhabditis elegans Proteins; D003001:Cloning, Molecular; D017931:DNA Primers; D005786:Gene Expression Regulation; D017238:Genes, Helminth; D005809:Genes, Regulator; D005838:Genotype; D015801:Helminth Proteins / Q000096:biosynthesis* / Q000235:genetics*; D008562:Membrane Glycoproteins / Q000096:biosynthesis* / Q000235:genetics; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000235:genetics*; D008957:Models, Genetic; D008969:Molecular Sequence Data; D016296:Mutagenesis; D009876:Operon; D016133:Polymerase Chain Reaction; D051880:Receptors, Notch; D015183:Restriction Mapping
chemical_list: D029742:Caenorhabditis elegans Proteins; D017931:DNA Primers; C060620:Glp-1 protein, C elegans; D015801:Helminth Proteins; C082460:Lin-12 protein, C elegans; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D051880:Receptors, Notch; C101221:SEL-1 protein, C elegans