title: The intratracheal administration of endotoxin: X. Dexamethasone downregulates neutrophil emigration and cytokine expression in vivo.
abstract: Intratracheal instillation of endotoxin (LPS) causes acute pulmonary inflammation characterized by the accumulation of plasma proteins and leukocytes within the pulmonary airways. The synthetic glucocorticoid dexamethasone 1) inhibits the LPS-initiated vascular leak of plasma proteins into the airspace, 2) inhibits the LPS-initiated emigration of neutrophils and lymphocytes into the airspace in a dose-dependent fashion, and 3) inhibits LPS-initiated mRNA and/or bronchoalveolar lavage protein expression of cytokines (TNF, IL-1 and IL-6) and chemokines (MIP-1 alpha, MIP-2 and MCP-1). In conclusion, dexamethasone inhibits both the vascular and cellular aspects of acute inflammation by downregulation of a broad spectrum of inflammatory cytokines and chemokines.
authors.affiliation: Department of Pathology University of California at San Diego School of Medicine 92103, USA.
authors.forename: E S
authors.initials: ES
authors.lastname: Yi
authors.forename: D G
authors.initials: DG
authors.lastname: Remick
authors.forename: Y
authors.initials: Y
authors.lastname: Lim
authors.forename: W
authors.initials: W
authors.lastname: Tang
authors.forename: C E
authors.initials: CE
authors.lastname: Nadzienko
authors.forename: A
authors.initials: A
authors.lastname: Bedoya
authors.forename: S
authors.initials: S
authors.lastname: Yin
authors.forename: T R
authors.initials: TR
authors.lastname: Ulich
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology* / Q000627:therapeutic use; D001427:Bacterial Toxins / Q000008:administration & dosage / Q000633:toxicity*; D001483:Base Sequence; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry / Q000166:cytology; D002478:Cells, Cultured; D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D016207:Cytokines / Q000096:biosynthesis* / Q000235:genetics; D003907:Dexamethasone / Q000494:pharmacology* / Q000627:therapeutic use; D004731:Endotoxins / Q000008:administration & dosage / Q000633:toxicity*; D005786:Gene Expression Regulation / Q000187:drug effects*; D007322:Instillation, Drug; D019272:Leukocyte Elastase / Q000032:analysis; D008070:Lipopolysaccharides / Q000008:administration & dosage / Q000633:toxicity*; D008297:Male; D008969:Molecular Sequence Data; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism; D016133:Polymerase Chain Reaction; D051381:Rats; D011917:Rats, Inbred Lew; D012128:Respiratory Distress Syndrome / Q000139:chemically induced* / Q000188:drug therapy / Q000473:pathology; D014132:Trachea
chemical_list: D000893:Anti-Inflammatory Agents; D001427:Bacterial Toxins; D016207:Cytokines; D004731:Endotoxins; D008070:Lipopolysaccharides; C027952:salmonella toxin; D003907:Dexamethasone; D019272:Leukocyte Elastase