Evaluating efficiency of bone marrow harvest and its manipulation--role of CD 34 positivity.
We evaluated harvested marrow cells for total nucleated cells (27.49 x 10(9)), absolute 'lymphocyte' count (6.29 x 10(9)) and CD 34 positive cells (3.57 x 10(9)). The same parameters were studied after in vitro manipulation to remove RBCs and plasma. Reinfused WBCs contained 12.87 x 10(9) nucleated cells, 4.25 x 10(9) absolute 'lymphocyutes' and 3.34 x 10(9) CD 34 positive cells. The corresponding figures for loss during in vitro manipulation (tubing, RBCs and plasma) are 14.62 (53.18%), 2.04 (32.43%) and 0.23 x 10(9) (6.44%) cells respectively. Therefore CD 34 positivity may be a better indicator of total yield, loss during manipulation and reinfusion of hemopoietic progenitor cells in bone marrow transplantation.
Parikh
P M
PM
Department of Medical Oncology, Tata Memorial Hospital, Parel, Bombay.
Badrinath
Y
Y
Dhond
S
S
Kulkarni
S
S
Dhar
A K
AK
Raje
N
N
Saikia
T K
TK
Bharbhaya
S
S
Gopal
R
R
Kurkure
P A
PA
D018952:Antigens, CD34 / Q000032:analysis*; D001854:Bone Marrow Cells; D001856:Bone Marrow Examination; D016465:Bone Marrow Purging; D016026:Bone Marrow Transplantation; D016238:Cytapheresis; D004912:Erythrocytes; D005260:Female; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000276:immunology; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000628:therapy; D007958:Leukocyte Count; D018655:Lymphocyte Count; D010949:Plasma; D010956:Plasmapheresis
D018952:Antigens, CD34