Karyotyping by PFGE of clinical isolates of Sporothrix schenckii.
From October 1991 to December 1992 we had eight patients with sporotrichosis at Tsukuba University Hospital in Japan. With 8 strains isolated from these patients, PFGE (pulsed-field gel electrophoresis) analyses were carried out to examine whether the karyotype of S. schenckii is distinguished by our method and whether this molecular approach is a useful means of biotyping of S. schenckii strains. Chromosomes were separated by contour-clamped homogeneous electric field (CHEF) gel electrophoresis. The strains had six to eight chromosomes and a total genome size was approx. 28 Mbp. Although these karyotypes of all the isolates looked closely similar to each other, they were grouped into three types.
Tateishi
T
T
Department of Dermatology, University of Tsukuba, School of Medicine, Ibaraki, Japan. ault-ttis@asahi-net.or.jp
Murayama
S Y
SY
Otsuka
F
F
Yamaguchi
H
H
D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods; D006801:Humans; D007621:Karyotyping / Q000379:methods; D013175:Sporothrix / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D013174:Sporotrichosis / Q000235:genetics* / Q000382:microbiology*