title: Characterization of an Escherichia coli rotA mutant, affected in periplasmic peptidyl-prolyl cis/trans isomerase.
abstract: The rotA gene of Escherichia coli encodes a peptidyl-prolyl cis/trans isomerase (PPIase), which is supposed to catalyse protein folding in the periplasm. To investigate the importance of the enzyme, the rotA gene was cloned and a chromosomal deletion mutant was created. The rotA mutant was normally viable. No residual PPIase activity could be detected in the periplasmic fraction of the mutant. Comparison of the patterns of periplasmic and outer membrane proteins by SDS-PAGE revealed no differences in protein composition between the rotA mutant and its parental strain. Similarly, the kinetics of periplasmic protein folding and outer membrane protein assembly appeared unaffected by the rotA mutation. Our results show that the periplasmic PPIase of E. coli is not essential and that the protein does not play an important role in protein folding.
authors.affiliation: Department of Molecular Cell Biology, Utrecht University, Netherlands.
authors.forename: M
authors.initials: M
authors.lastname: Kleerebezem
authors.forename: M
authors.initials: M
authors.lastname: Heutink
authors.forename: J
authors.initials: J
authors.lastname: Tommassen
mesh_terms: D000591:Amino Acid Isomerases / Q000737:chemistry / Q000235:genetics*; D015139:Blotting, Southern; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics*; D003001:Cloning, Molecular; D004790:Enzyme Induction / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D009154:Mutation; D019696:Peptidylprolyl Isomerase; D010641:Phenotype; D017510:Protein Folding
chemical_list: D002352:Carrier Proteins; D000591:Amino Acid Isomerases; D019696:Peptidylprolyl Isomerase