Enhanced ornithine decarboxylase activity of chick muscle cells in culture by beta-adrenergic agonists.
The ability of beta-adrenergic agonists to stimulate ornithine decarboxylase activity (ODC) in chick muscle cell culture prepared from 11-day old embryos was evaluated. After 72 h of preincubation (myotube formation) the medium was supplemented for 4 h with noradrenaline, ritodrine, isoproterenol or clenbuterol, at concentrations of 10(-12), 10(-9) and 10(-6) mol/l. No significant response of ODC activity to noradrenaline was observed. The highest concentration (10(-6) mol/l) of the beta-adrenergic agonists ritodrine and isoproterenol elevated the activity of ODC. Clenbuterol was the most active beta-adrenergic agonist. The lowest concentration (10(-12) mol/l) had an apparent effect on ODC activity in muscle cell culture, and the substitution of media at levels of 10(-9) and 10(-6) mol/l had a similar effect in comparison to controls. The potency of beta-adrenergic agonists in increasing ODC activity was on the following order: noradrenaline, ritodrine, isoproterenol, clenbuterol. Results indicate that beta-adrenergic agonists may directly stimulate ODC activity followed by physiological processes in the muscle cells in the early stage of chick embryonic development.
Juráni
M
M
Institute of Animal Biochemistry and Genetics, Slovak Academy of Sciences, Slovakia.
Vaneková
M
M
Vyboh
P
P
Lamosová
D
D
D000318:Adrenergic beta-Agonists / Q000494:pharmacology*; D000704:Analysis of Variance; D000818:Animals; D018929:Cell Culture Techniques; D002642:Chick Embryo; D018482:Muscle, Skeletal / Q000166:cytology / Q000187:drug effects / Q000201:enzymology*; D009955:Ornithine Decarboxylase / Q000096:biosynthesis*
D000318:Adrenergic beta-Agonists; D009955:Ornithine Decarboxylase